LOCUS       DAZFKY010000001        98120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFKY010000001 DAZFKY010000000
VERSION     DAZFKY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..326
                     /locus_tag="ACHKCL_000001"
     CDS             105..326
                     /locus_tag="ACHKCL_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5761056.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            323..1270
                     /locus_tag="ACHKCL_000002"
     CDS             323..1270
                     /locus_tag="ACHKCL_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5761057.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            1272..1448
                     /locus_tag="ACHKCL_000003"
     CDS             1272..1448
                     /locus_tag="ACHKCL_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761058.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            1782..2138
                     /locus_tag="ACHKCL_000004"
     CDS             1782..2138
                     /locus_tag="ACHKCL_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761059.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            2135..2497
                     /locus_tag="ACHKCL_000005"
     CDS             2135..2497
                     /locus_tag="ACHKCL_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5761060.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEP
                     ASKVTHWMPLPEPPQEVK"
     gene            2585..2827
                     /locus_tag="ACHKCL_000006"
     CDS             2585..2827
                     /locus_tag="ACHKCL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5761061.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2831..2965
                     /locus_tag="ACHKCL_000007"
     CDS             2831..2965
                     /locus_tag="ACHKCL_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761062.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            2984..3238
                     /gene="xisR"
                     /locus_tag="ACHKCL_000008"
     CDS             2984..3238
                     /gene="xisR"
                     /locus_tag="ACHKCL_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5761063.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3272..4558
                     /locus_tag="ACHKCL_000009"
     CDS             3272..4558
                     /locus_tag="ACHKCL_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5761064.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4579..5280
                     /gene="mlrA"
                     /locus_tag="ACHKCL_000010"
     CDS             4579..5280
                     /gene="mlrA"
                     /locus_tag="ACHKCL_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5761065.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            5340..5447
                     /gene="yohO"
                     /locus_tag="ACHKCL_000011"
     CDS             5340..5447
                     /gene="yohO"
                     /locus_tag="ACHKCL_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5761066.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(5428..6159)
                     /gene="yehW"
                     /locus_tag="ACHKCL_000012"
     CDS             complement(5428..6159)
                     /gene="yehW"
                     /locus_tag="ACHKCL_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5761067.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(6164..7090)
                     /gene="yehX"
                     /locus_tag="ACHKCL_000013"
     CDS             complement(6164..7090)
                     /gene="yehX"
                     /locus_tag="ACHKCL_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5761068.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(7083..8240)
                     /gene="yehY"
                     /locus_tag="ACHKCL_000014"
     CDS             complement(7083..8240)
                     /gene="yehY"
                     /locus_tag="ACHKCL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5761069.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(8247..9164)
                     /gene="osmF"
                     /locus_tag="ACHKCL_000015"
     CDS             complement(8247..9164)
                     /gene="osmF"
                     /locus_tag="ACHKCL_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5761070.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(9416..11713)
                     /gene="bglX"
                     /locus_tag="ACHKCL_000016"
     CDS             complement(9416..11713)
                     /gene="bglX"
                     /locus_tag="ACHKCL_000016"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5761071.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11909..13624
                     /gene="dld"
                     /locus_tag="ACHKCL_000017"
     CDS             11909..13624
                     /gene="dld"
                     /locus_tag="ACHKCL_000017"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5761072.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13662..14594)
                     /gene="pbpG"
                     /locus_tag="ACHKCL_000018"
     CDS             complement(13662..14594)
                     /gene="pbpG"
                     /locus_tag="ACHKCL_000018"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5761073.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14768..15355)
                     /gene="yohC"
                     /locus_tag="ACHKCL_000019"
     CDS             complement(14768..15355)
                     /gene="yohC"
                     /locus_tag="ACHKCL_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5761074.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            15525..16103
                     /gene="yohD"
                     /locus_tag="ACHKCL_000020"
     CDS             15525..16103
                     /gene="yohD"
                     /locus_tag="ACHKCL_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5761075.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(16233..16994)
                     /gene="yohF"
                     /locus_tag="ACHKCL_000021"
     CDS             complement(16233..16994)
                     /gene="yohF"
                     /locus_tag="ACHKCL_000021"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5761076.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(17047..18483)
                     /gene="mdtQ"
                     /locus_tag="ACHKCL_000022"
     CDS             complement(17047..18483)
                     /gene="mdtQ"
                     /locus_tag="ACHKCL_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5761077.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            18707..18790
                     /gene="yohP"
                     /locus_tag="ACHKCL_000023"
     CDS             18707..18790
                     /gene="yohP"
                     /locus_tag="ACHKCL_000023"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5761078.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(19178..20128)
                     /gene="dusC"
                     /locus_tag="ACHKCL_000024"
     CDS             complement(19178..20128)
                     /gene="dusC"
                     /locus_tag="ACHKCL_000024"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5761079.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(20288..21481)
                     /locus_tag="ACHKCL_000025"
     CDS             complement(20288..21481)
                     /locus_tag="ACHKCL_000025"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO5761080.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(21496..22140)
                     /gene="maiA"
                     /locus_tag="ACHKCL_000026"
     CDS             complement(21496..22140)
                     /gene="maiA"
                     /locus_tag="ACHKCL_000026"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO5761081.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(22149..22850)
                     /locus_tag="ACHKCL_000027"
     CDS             complement(22149..22850)
                     /locus_tag="ACHKCL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5761082.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(22865..23893)
                     /gene="gtdA"
                     /locus_tag="ACHKCL_000028"
     CDS             complement(22865..23893)
                     /gene="gtdA"
                     /locus_tag="ACHKCL_000028"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO5761083.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(23905..25263)
                     /locus_tag="ACHKCL_000029"
     CDS             complement(23905..25263)
                     /locus_tag="ACHKCL_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761084.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            25390..26298
                     /locus_tag="ACHKCL_000030"
     CDS             25390..26298
                     /locus_tag="ACHKCL_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5761085.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            26429..26827
                     /gene="yohJ"
                     /locus_tag="ACHKCL_000031"
     CDS             26429..26827
                     /gene="yohJ"
                     /locus_tag="ACHKCL_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5761086.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            26824..27519
                     /gene="yohK"
                     /locus_tag="ACHKCL_000032"
     CDS             26824..27519
                     /gene="yohK"
                     /locus_tag="ACHKCL_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5761087.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            27649..28533
                     /gene="cdd"
                     /locus_tag="ACHKCL_000033"
     CDS             27649..28533
                     /gene="cdd"
                     /locus_tag="ACHKCL_000033"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5761088.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            28683..29402
                     /gene="sanA"
                     /locus_tag="ACHKCL_000034"
     CDS             28683..29402
                     /gene="sanA"
                     /locus_tag="ACHKCL_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5761089.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            29405..29644
                     /gene="yeiS"
                     /locus_tag="ACHKCL_000035"
     CDS             29405..29644
                     /gene="yeiS"
                     /locus_tag="ACHKCL_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5761090.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            29838..31076
                     /gene="preT"
                     /locus_tag="ACHKCL_000036"
     CDS             29838..31076
                     /gene="preT"
                     /locus_tag="ACHKCL_000036"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5761091.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            31070..32305
                     /gene="preA"
                     /locus_tag="ACHKCL_000037"
     CDS             31070..32305
                     /gene="preA"
                     /locus_tag="ACHKCL_000037"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5761092.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(32548..33558)
                     /gene="mglC"
                     /locus_tag="ACHKCL_000038"
     CDS             complement(32548..33558)
                     /gene="mglC"
                     /locus_tag="ACHKCL_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5761093.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33574..35094)
                     /gene="mglA"
                     /locus_tag="ACHKCL_000039"
     CDS             complement(33574..35094)
                     /gene="mglA"
                     /locus_tag="ACHKCL_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5761094.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(35155..36153)
                     /gene="mglB"
                     /locus_tag="ACHKCL_000040"
     CDS             complement(35155..36153)
                     /gene="mglB"
                     /locus_tag="ACHKCL_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5761095.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(36432..37472)
                     /gene="galS"
                     /locus_tag="ACHKCL_000041"
     CDS             complement(36432..37472)
                     /gene="galS"
                     /locus_tag="ACHKCL_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5761096.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(37614..38771)
                     /gene="yeiB"
                     /locus_tag="ACHKCL_000042"
     CDS             complement(37614..38771)
                     /gene="yeiB"
                     /locus_tag="ACHKCL_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5761097.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(38788..39456)
                     /gene="folE"
                     /locus_tag="ACHKCL_000043"
     CDS             complement(38788..39456)
                     /gene="folE"
                     /locus_tag="ACHKCL_000043"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5761098.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            39714..40550
                     /gene="yeiG"
                     /locus_tag="ACHKCL_000044"
     CDS             39714..40550
                     /gene="yeiG"
                     /locus_tag="ACHKCL_000044"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5761099.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(40582..42561)
                     /gene="cirA"
                     /locus_tag="ACHKCL_000045"
     CDS             complement(40582..42561)
                     /gene="cirA"
                     /locus_tag="ACHKCL_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO5761100.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(42853..44322)
                     /gene="lysP"
                     /locus_tag="ACHKCL_000046"
     CDS             complement(42853..44322)
                     /gene="lysP"
                     /locus_tag="ACHKCL_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5761101.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(44527..45408)
                     /gene="yieE"
                     /locus_tag="ACHKCL_000047"
     CDS             complement(44527..45408)
                     /gene="yieE"
                     /locus_tag="ACHKCL_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5761102.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            45507..46556
                     /gene="yeiH"
                     /locus_tag="ACHKCL_000048"
     CDS             45507..46556
                     /gene="yeiH"
                     /locus_tag="ACHKCL_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5761103.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            46630..47487
                     /gene="nfo"
                     /locus_tag="ACHKCL_000049"
     CDS             46630..47487
                     /gene="nfo"
                     /locus_tag="ACHKCL_000049"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5761104.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            47490..48578
                     /gene="yeiI"
                     /locus_tag="ACHKCL_000050"
     CDS             47490..48578
                     /gene="yeiI"
                     /locus_tag="ACHKCL_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5761105.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(48634..49884)
                     /gene="nupX"
                     /locus_tag="ACHKCL_000051"
     CDS             complement(48634..49884)
                     /gene="nupX"
                     /locus_tag="ACHKCL_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5761106.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49984..50925)
                     /gene="rihB"
                     /locus_tag="ACHKCL_000052"
     CDS             complement(49984..50925)
                     /gene="rihB"
                     /locus_tag="ACHKCL_000052"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5761107.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            51055..51753
                     /gene="yeiL"
                     /locus_tag="ACHKCL_000053"
     CDS             51055..51753
                     /gene="yeiL"
                     /locus_tag="ACHKCL_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5761108.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(51824..53074)
                     /locus_tag="ACHKCL_000054"
     CDS             complement(51824..53074)
                     /locus_tag="ACHKCL_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5761109.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(53168..54106)
                     /gene="psuG"
                     /locus_tag="ACHKCL_000055"
     CDS             complement(53168..54106)
                     /gene="psuG"
                     /locus_tag="ACHKCL_000055"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5761110.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(54094..55035)
                     /gene="psuK"
                     /locus_tag="ACHKCL_000056"
     CDS             complement(54094..55035)
                     /gene="psuK"
                     /locus_tag="ACHKCL_000056"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5761111.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(55459..57150)
                     /gene="fruA"
                     /locus_tag="ACHKCL_000057"
     CDS             complement(55459..57150)
                     /gene="fruA"
                     /locus_tag="ACHKCL_000057"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5761112.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(57167..58105)
                     /gene="fruK"
                     /locus_tag="ACHKCL_000058"
     CDS             complement(57167..58105)
                     /gene="fruK"
                     /locus_tag="ACHKCL_000058"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5761113.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(58105..59235)
                     /gene="fruB"
                     /locus_tag="ACHKCL_000059"
     CDS             complement(58105..59235)
                     /gene="fruB"
                     /locus_tag="ACHKCL_000059"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5761114.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            59603..60784
                     /gene="setB"
                     /locus_tag="ACHKCL_000060"
     CDS             59603..60784
                     /gene="setB"
                     /locus_tag="ACHKCL_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5761115.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(60781..61035)
                     /gene="yeiW"
                     /locus_tag="ACHKCL_000061"
     CDS             complement(60781..61035)
                     /gene="yeiW"
                     /locus_tag="ACHKCL_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5761116.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            61190..61762
                     /gene="yeiP"
                     /locus_tag="ACHKCL_000062"
     CDS             61190..61762
                     /gene="yeiP"
                     /locus_tag="ACHKCL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5761117.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            61985..63451
                     /gene="yeiQ"
                     /locus_tag="ACHKCL_000063"
     CDS             61985..63451
                     /gene="yeiQ"
                     /locus_tag="ACHKCL_000063"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5761118.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            63569..64555
                     /gene="yeiR"
                     /locus_tag="ACHKCL_000064"
     CDS             63569..64555
                     /gene="yeiR"
                     /locus_tag="ACHKCL_000064"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5761119.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            64594..65307
                     /gene="lpxT"
                     /locus_tag="ACHKCL_000065"
     CDS             64594..65307
                     /gene="lpxT"
                     /locus_tag="ACHKCL_000065"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5761120.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            65720..66286
                     /gene="mepS"
                     /locus_tag="ACHKCL_000066"
     CDS             65720..66286
                     /gene="mepS"
                     /locus_tag="ACHKCL_000066"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5761121.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            66467..68023
                     /gene="pdeN"
                     /locus_tag="ACHKCL_000067"
     CDS             66467..68023
                     /gene="pdeN"
                     /locus_tag="ACHKCL_000067"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5761122.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            68105..69919
                     /gene="yejA"
                     /locus_tag="ACHKCL_000068"
     CDS             68105..69919
                     /gene="yejA"
                     /locus_tag="ACHKCL_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5761123.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            69920..71014
                     /gene="yejB"
                     /locus_tag="ACHKCL_000069"
     CDS             69920..71014
                     /gene="yejB"
                     /locus_tag="ACHKCL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5761124.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            71014..72039
                     /gene="yejE"
                     /locus_tag="ACHKCL_000070"
     CDS             71014..72039
                     /gene="yejE"
                     /locus_tag="ACHKCL_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5761125.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            72041..73630
                     /gene="yejF"
                     /locus_tag="ACHKCL_000071"
     CDS             72041..73630
                     /gene="yejF"
                     /locus_tag="ACHKCL_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5761126.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(73634..73978)
                     /gene="yejG"
                     /locus_tag="ACHKCL_000072"
     CDS             complement(73634..73978)
                     /gene="yejG"
                     /locus_tag="ACHKCL_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5761127.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(74311..75501)
                     /gene="bcr"
                     /locus_tag="ACHKCL_000073"
     CDS             complement(74311..75501)
                     /gene="bcr"
                     /locus_tag="ACHKCL_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5761128.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(75529..76224)
                     /gene="rsuA"
                     /locus_tag="ACHKCL_000074"
     CDS             complement(75529..76224)
                     /gene="rsuA"
                     /locus_tag="ACHKCL_000074"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5761129.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            76373..78133
                     /gene="radD"
                     /locus_tag="ACHKCL_000075"
     CDS             76373..78133
                     /gene="radD"
                     /locus_tag="ACHKCL_000075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5761130.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            78258..78542
                     /gene="rplY"
                     /locus_tag="ACHKCL_000076"
     CDS             78258..78542
                     /gene="rplY"
                     /locus_tag="ACHKCL_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5761131.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(78681..79688)
                     /gene="yejK"
                     /locus_tag="ACHKCL_000077"
     CDS             complement(78681..79688)
                     /gene="yejK"
                     /locus_tag="ACHKCL_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5761132.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            79870..80097
                     /gene="yejL"
                     /locus_tag="ACHKCL_000078"
     CDS             79870..80097
                     /gene="yejL"
                     /locus_tag="ACHKCL_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5761133.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            80117..81877
                     /gene="yejM"
                     /locus_tag="ACHKCL_000079"
     CDS             80117..81877
                     /gene="yejM"
                     /locus_tag="ACHKCL_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5761134.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            81952..82028
                     /locus_tag="ACHKCL_000080"
     tRNA            81952..82028
                     /locus_tag="ACHKCL_000080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81986..81988,aa:Pro,seq:ggg)
     gene            complement(82129..84720)
                     /gene="yejO"
                     /locus_tag="ACHKCL_000081"
     CDS             complement(82129..84720)
                     /gene="yejO"
                     /locus_tag="ACHKCL_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO5761135.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            85040..85687
                     /gene="narP"
                     /locus_tag="ACHKCL_000082"
     CDS             85040..85687
                     /gene="narP"
                     /locus_tag="ACHKCL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5761136.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(85722..86774)
                     /gene="ccmH"
                     /locus_tag="ACHKCL_000083"
     CDS             complement(85722..86774)
                     /gene="ccmH"
                     /locus_tag="ACHKCL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5761137.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(86771..87328)
                     /gene="dsbE"
                     /locus_tag="ACHKCL_000084"
     CDS             complement(86771..87328)
                     /gene="dsbE"
                     /locus_tag="ACHKCL_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5761138.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(87325..89268)
                     /gene="ccmF"
                     /locus_tag="ACHKCL_000085"
     CDS             complement(87325..89268)
                     /gene="ccmF"
                     /locus_tag="ACHKCL_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5761139.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(89265..89744)
                     /gene="ccmE"
                     /locus_tag="ACHKCL_000086"
     CDS             complement(89265..89744)
                     /gene="ccmE"
                     /locus_tag="ACHKCL_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5761140.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(89741..89950)
                     /gene="ccmD"
                     /locus_tag="ACHKCL_000087"
     CDS             complement(89741..89950)
                     /gene="ccmD"
                     /locus_tag="ACHKCL_000087"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5761141.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(89947..90684)
                     /gene="ccmC"
                     /locus_tag="ACHKCL_000088"
     CDS             complement(89947..90684)
                     /gene="ccmC"
                     /locus_tag="ACHKCL_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5761142.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(90726..91388)
                     /gene="ccmB"
                     /locus_tag="ACHKCL_000089"
     CDS             complement(90726..91388)
                     /gene="ccmB"
                     /locus_tag="ACHKCL_000089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5761143.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(91385..92008)
                     /gene="ccmA"
                     /locus_tag="ACHKCL_000090"
     CDS             complement(91385..92008)
                     /gene="ccmA"
                     /locus_tag="ACHKCL_000090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5761144.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(92021..92623)
                     /gene="napC"
                     /locus_tag="ACHKCL_000091"
     CDS             complement(92021..92623)
                     /gene="napC"
                     /locus_tag="ACHKCL_000091"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5761145.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(92633..93082)
                     /gene="napB"
                     /locus_tag="ACHKCL_000092"
     CDS             complement(92633..93082)
                     /gene="napB"
                     /locus_tag="ACHKCL_000092"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5761146.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(93079..93942)
                     /gene="napH"
                     /locus_tag="ACHKCL_000093"
     CDS             complement(93079..93942)
                     /gene="napH"
                     /locus_tag="ACHKCL_000093"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5761147.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(93929..94624)
                     /gene="napG"
                     /locus_tag="ACHKCL_000094"
     CDS             complement(93929..94624)
                     /gene="napG"
                     /locus_tag="ACHKCL_000094"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5761148.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(94631..97117)
                     /gene="napA"
                     /locus_tag="ACHKCL_000095"
     CDS             complement(94631..97117)
                     /gene="napA"
                     /locus_tag="ACHKCL_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO5761149.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(97114..97377)
                     /gene="napD"
                     /locus_tag="ACHKCL_000096"
     CDS             complement(97114..97377)
                     /gene="napD"
                     /locus_tag="ACHKCL_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5761150.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(97367..97861)
                     /gene="napF"
                     /locus_tag="ACHKCL_000097"
     CDS             complement(97367..97861)
                     /gene="napF"
                     /locus_tag="ACHKCL_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5761151.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            97970..>98120
                     /gene="yojO"
                     /locus_tag="ACHKCL_000098"
     CDS             97970..>98120
                     /gene="yojO"
                     /locus_tag="ACHKCL_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5761152.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCS"
BASE COUNT        24097 a        26286 c        24377 g        23360 t
ORIGIN      
        1 cgatgaacat gaaaagcaaa atcatcaggg agctacaggc tcctttttta ttgttcgcat
       61 tcaccctcaa gcgtattaac caacaattca gggattaatg gaagatggca gacatcattg
      121 attcagcatc agaaattgaa gaattacagc gcaatacagc aataaaaatg cgtcgcctga
      181 actaccagac tgtatccgca actcattgtt gtgagtgtgg cgatccgata gatgaacgaa
      241 gacgcctggc agttcagggt tgtcggactt gtgcaagttg ccaggaggat ctggagctta
      301 tcagtaaaca gagaggttcg aagtgagcga aattaattat caggcactgc gtgaaaaagc
      361 agagaaagca actaaaggaa gctacatcgt agggcataca tctgttaacc agcacggcaa
      421 tttaacagga gtttttgttt gccaaaaatg gaaaggagaa cccggtggtg tgattgcgga
      481 atgtcatgtt aactgcctgg ttgaaacaga tgttcaggct tatgcaaacg ctgaatttat
      541 tgctgccttt aatccaaatg ttgcgctggc gcttctggat gaacgggaaa gaaaccagca
      601 atacatcaaa cgccgcgacc aggagaacga ggagattgcg cttacggttg ggaagctgcg
      661 tgttgagctt gaagcagcaa aatcaaaact caacgagcag cgtgaatatt acgagggagt
      721 aatcgcggat ggaagtaagc gcatagcaga actggaaaaa caatgcgccg aatgggagcg
      781 aaaagcatta agcaactttg aagagtgtgc tgcgatggct gaacgtatcg aagagatgca
      841 gacaaaatct gcaccagatt cgtttggcat catcggtgaa aatattcgaa cacaggacaa
      901 tcgaataacg tcagatccca tgttttgtgt gtatcaaaag cgcgaaatcg ctgttgatgc
      961 tgattatgac catgaccgga ttgtctgggt tgacgaagat ggcaatgaag ccaataaacg
     1021 ccatagtcgt cgtctcgagc tacttcatga aaactttcga gagccaccag aaaaatggcg
     1081 gcgcgttgct gtgaaagata ttgatgaatt cgttacctgc tgtttcaccg aacagggttg
     1141 taaagactac ctggcagtca atggtcacaa tcttcgcttg ccatttatat atgtaaaaag
     1201 cggtttcagg aacgctgaat atatcggcat aagaaactgg cttgctggca ttcgcatcaa
     1261 aggagagtga tatggcgtta acacaccgcg aactctgtca gattgcgtac aagttcctta
     1321 agcgcaacgg tttcaaggtt tgttttcatg accgctttat agctgtaacc agtaccggag
     1381 aacagccaga tgctatggga ttcagaaatt cagcatcatg cctgattggc gaaatgttct
     1441 cgtgctgact tgttggcaga tagaaaaaag cgttttcgta aaaatccgtc tcttggaatg
     1501 ggcgactggc gattctttat tagtgagccg ggaattattt caattgagga tttaccacct
     1561 ggctggggat tacttcacgt tgttaacgga agagtacgga aagtacatgg gtggcccaag
     1621 ggtaattgct gttggggtaa tcctgacgat aagccattta ctggaaataa gcaggttgaa
     1681 tgcgattaca tgttgtctgc attaaggcgc atggagttga gagggcacct taatgaaata
     1741 tatgacggtg taattgttaa taagaaagaa ggaaacgcgg catgaccact attaccgaca
     1801 aagaactgat taaagaaatc aaagagcgca taggcagctt ggacgttcga gacaatattg
     1861 agcgccgtgc ttatgaaatt gcactggcat cgctggaagc agaaccgata gcgtgggaat
     1921 gcggtgaaaa cataatcctg tttaaccctg acacagttga agcatacgca aaacgtgcgg
     1981 aaatatcacc taaaccacta ttctccgcgc cgccagcgct ggtagtgcct gataagttgc
     2041 cgcgtgaata cagaaacggt tggcctcttg cgtatagtga ttatgctgaa ggctggaacg
     2101 actgccgcga agccatgctt cagggagata aatcatgatt aatcgtatca agctggagca
     2161 catcctcgaa tatgccaggc agcagaggca tattggtcag cattgtaaaa ttccaccagg
     2221 agatatggtt gaaatcatgg agattgccat gcgcaaggct ggcaactctc cggtaactcc
     2281 ggatggttgg ataagctgta gtgagcgaat gccgaacgat aaacagtatg tttggtgttg
     2341 ggggaagtct tacggctgga ctgagtgcga taccttcgaa gggtattacg attgttcgag
     2401 aaacaaatgg tgggcagtta ctgacaatgg ggaagaaccg gcatcgaaag taacccactg
     2461 gatgccgcta ccggagccgc cgcaggaggt gaaatgatga attggcctga agcattcacc
     2521 gcagttggag ttgcaatagc ggtggcattt attctgtatt cgcttttccg ctggggataa
     2581 aggaatgttc gctctgattc aacgtggtca gatatacacg gacagagccg gataccctgt
     2641 ggtgattact cgcagtactc agcactcagt gttctttcga cgcatggacg ggcgctccgg
     2701 acgggtacgc attggtgagt tcaacaacct gttcgaacat attgaccaac aggagtaccg
     2761 caaaattctg gcgggcactg agcaggaaat gcgcctgaaa aaattacgcg caatgcaacg
     2821 gaggtgatac atgcatacgg cttttgagtt ctgggttcgc aagacattcg gcaatcgcta
     2881 cgacctgacc cgtgatgtcg acggcttcta ctgccgtgaa gttgtgaaac gaatgtttga
     2941 cgtgtggtgc cactgccgtg gatgaaagtt ttatgaggtt ggcatgcaga caatcatcta
     3001 tcagataacc cccagcaaat ggtgtacgga gagagtcctt attgcatcaa cagggctaaa
     3061 gcccggcacc atcgagcggg ccagaagaaa gtcatggatg cagggaaaag aataccgcca
     3121 ttacgctgta gaaggtgatc ctgggcatta cagtgaatgc ctgtacaaca tcgaagaaat
     3181 tatgcgatgg atcgaaaacc agaaacaacc aggtgccaaa aatgcaagtt ccggttaacc
     3241 tgttaatgct cctggacgtc tgggaggttt aatgagtaac gcatcatacc cgacaggcgt
     3301 tgaaaaccat ggcggatcac tccgtatatg gtttcactat aatggcaaac gtgtcagaga
     3361 aaacctcggt gttcctgaca cagccaaaaa ccggaagatc gctggtgaac ttcgcacttc
     3421 cgtttgtttt gcaatcagaa tggggagttt cgactacgcc gcgcagttcc ctaattcccc
     3481 taacctgaaa cactttggtc tgggaaaaag agagataacc gttaaggcac tttcggaaaa
     3541 atggttggac cttaagaaaa ttgagatttg tgcgaatgca cttaaccgtt accagtcagt
     3601 aattaaaaac atgttaccaa tgttaggtga aaaaaaactg gtttcatcca taacaaaaga
     3661 ggatttactt ttcgtaagga gagatttgtt gaccggttac caaaagcttt ctaatggaaa
     3721 gacttcttcc ataaaagggc gctcagtggt cacggtaaac tactatatga caaccatagc
     3781 tggaatgttt caatttgcaa cagataatgg ttatacctca ggaaacccat ttaacggtct
     3841 ggctccctta aaaaagtcca aggtaaaacc agatcctctc acccgtgacg aatttattcg
     3901 ttttattgag gcttgccgtc atcaacaaac aaaaaacctg tggattctcg ctgtatacac
     3961 gggtattcgt cacggggagt tggtatcgct ggcatgggaa gatatagacc ttaaagcaag
     4021 gactataacc atccgtagaa attatacaaa acttggcgaa ttcactccac caaaaaccga
     4081 tgcaggcacc ggaaggacaa ttcatctggt tcaaccagct attgatgctc ttaaaagcca
     4141 ggcggaaatg accatgcttg gaaagcaaca ttctgtagag gtgaagcaga gggaatatgg
     4201 gagaactgct gtgcataaat gcacttttgt ttttagtcct caggtaacaa aacagcagca
     4261 gttgtccgga cctcactaca aggttgactc catcagggag tcatggacaa gtatcttaaa
     4321 acgcgcaggt ctgagacaca gaaaatcgta ccaatccagg catacttatg catgctggtc
     4381 acttgccgca ggagctaatc ctagttttat cgcaagccag atgggccaca caaacgcaca
     4441 aatggtattc aatgtttacg gagcatggat gaaagacaac aatcacgaac agatagaact
     4501 ccttaacaaa agactatctg aaagtgtccc atgtatgccc cataagaaag cagggtaaaa
     4561 taaaaacttg caaaatcaat tagtttaccc ttaatccctg tcacgttacg cgcgtggcag
     4621 aggcgttacg gattgctgaa accgcaacgg acagacggcg gtcatcggct gttcaacgat
     4681 gccgatattg accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc
     4741 aaagttaaaa tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag
     4801 caagaaacat tactgaccta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc
     4861 aaagagcgcg gtcaggatta ccccgcccag acgctcacca cacatctgtt tattcctctg
     4921 cgccgacggc ttcagtacca acaaccgact ctccaggcgc tgctggcgat cctcgacggc
     4981 gtactgatca actacatcgc catttgtctg gcttcggcac gtaaaaaaca gggtaaagat
     5041 gcgctggtgg ttggctggaa tattcaggat accacccgtc tgtggctgga gggctggatt
     5101 gccagtcaac aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct
     5161 gaactattcg aaggccgtac attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa
     5221 cagcagcaac tcaccagttg gcaagaacaa ggccatgata ttttcccact cggcatttaa
     5281 tgattcgtta acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta
     5341 tgcgcatagc taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg
     5401 gtggcgtcat gctcgcaggt tataccttta ttttgcgtgc tggctaagcg cctgcaccag
     5461 cctttcaaac aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ccccctggat
     5521 cacatacgcg gtattaaatc cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc
     5581 ccctaccgtt gaggcgatcg tcgccgtacc aatgttgata atcaccgagg ttcgcacgcc
     5641 cgccagaatc accggagccg ccagcggtag ctcgacctta cgcagtcgct gacctcgact
     5701 cattcccata cctttcgcaa cttctgtcac gctggcatca atcgctccca gcccggcaag
     5761 tgtcgcctgc aggacgggca gcacaccata aaggatcaag gcgataatcg ctggttgcag
     5821 accaaagcct atcaccggaa cagcaatcgc cagcactgcg acaggcggaa aagtctgccc
     5881 aacggcggca atagtttcca ccagtgggcg aaattccgcg ccccacgggc gagtgactgc
     5941 aattccggca ccagtgccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa
     6001 atgagccagt gccaaagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg
     6061 gaacaaggca gcaaacagcg gctggctgta aggcagccag aaaatcagtg ccacaaacaa
     6121 agcaatgagc cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tccaccagca
     6181 gatcctgaaa atgcagcgtg ccgctaggct ggccctgcgt gttcaccacc ggcagcacct
     6241 cgcatccccg cgcgacaaac agggagagcg catcgcgcag cgtcatctct tctgccagtg
     6301 cctcaccttc tgcccgttct tcgcgacgca cgtaatccgc cacactacgt aacgaaagca
     6361 ggcgcacacc cagttcacta cgtccaaaaa actggcggac aaaatcattc gccggacgag
     6421 tcagcatcgt cagcggattc ccctgctgca ccacttcacc gtgatccatc aataccagat
     6481 gttctgccag ccgtagcgcc tcatcaatat catgcgtgac cagcacaatg gtacgcccca
     6541 gcaaacggtg aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg
     6601 cgccaaaagg ttcatccatc agtaagactt gcggatcggc agccagtgcg cgcgctactc
     6661 ccacacgttg ctgctgacca ccggaaagct gatgtggata acgctcacgt aaatttgact
     6721 ccagccccag tagcgccatt aattcgtcga tacgatcgtc aatcctcgcc cgcgaccatt
     6781 tttgtaattg cggcacggtg gcaatgtttt gtgccacgct ccagtgggga aacaggccaa
     6841 tagattgaat ggcatagccc atacggcggc gcaactccag tactggcagc gagcgaattt
     6901 cttctccagc aaagcggagc tctccgctgt catgttccac caggcggtta atcattttca
     6961 gggtggtgga tttgccggag ccagatgtgc caatcagcac cgaaaaactc ccttcctgaa
     7021 aattgagatt gagatcgtta acggcttttt gtgcgccgaa cagtttgctg acatggctaa
     7081 attcaatcat tacgtttcac cttcaacagt gcgattagta aatcgaacag cgcatcgatc
     7141 agcaccgcca gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt
     7201 agcagcccct ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc
     7261 gccatgccta cagtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc
     7321 agcggtaact gaacatgcag gaatcgctgc gccccgctca tgcccatcgc tctggcgctc
     7381 tccagcacat cgcgcgggat ctggttcaag ccgactacca cgccgcgcac cagcggtagc
     7441 aaggcataga gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc tatgccaagc
     7501 ttccccagcc acggaaaggc cgtaaccagt ccggcaagcg gcgcaatcag caggccaaag
     7561 agcgccacgg aaggcacggt ctgaatgaca ttgagcagag aaaaaatcgc cccctgccgc
     7621 gcggtggaaa agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc
     7681 gcaccaaaca gcaaggtcag atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg
     7741 gcgtattctt tcattagtga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga
     7801 ataatggcaa tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg
     7861 atggcatcgc tacaggccag caatgccagc gccgaagcca accagaaacc actgccgagg
     7921 ctggtacgcg ccagcgcact gccattttgc gccagttggg tcgccgcctt tccagctccc
     7981 cacaccagca atacgaagac gaattgcgcc agaatgagtg caaaaatgct gcctttttta
     8041 gcgggaataa aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagatcgtt
     8101 tgcggccaca gttgccagag atgacgccct tcacccgaga ctaaacgatt aggcgcgtag
     8161 ctgataaacg gcagcgctgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga
     8221 ttaatacgta gataagtcac gggaaattac ttcgtccacc ctttttgttt caagtagtcg
     8281 gcagccactt ttttggcatc cagtccttcc actgcaatgc tggcattcag ttgctgcaat
     8341 gtttttgcat cgaggctggc gaagactggc tgtagccact gtgccatttg cggatactct
     8401 ttcagcaccg actcacgcac cactggtgca ggcgcgtaga taggttgcac gccttgcgga
     8461 tcgcttaagg tttgcagccc ctgcgccgcg accggaccgt cagtgccgta agccattgcg
     8521 gcattaacgc cagaagtttg ctgggcagcg gctttaatcg ttaccgccgt gtcgccgcca
     8581 gccagtgaca gtaactgatc ctgatcgagt ttaaagtcgt aggctttttc aaacgcgggt
     8641 aacgcatcgg cgcgttcgat aaactcagcc gaggctgcca gtttgaaggt gccgccctct
     8701 ttcagataac gcctcaggtc agcaagcgaa gtgagtttgt ttttctctgc cacatcctga
     8761 cgcacggcga tggtccaggt gttatttgca ggtgcgggcg tcagccagat taacttgttt
     8821 tgctctgcat cgagtttttt gactttctcg taaccttgct gcgcgttttt ccacgctgca
     8881 tcgttttcat ctttaaagaa aaacgcgcca ttgccggtat attccggata gatatccagt
     8941 tcaccggaag taatcgcccc gcgcaccaca ggagtcgttc caagttgcac tttattgacc
     9001 gttggtactc cgtggctttc gagtacttgc aaaatgatat tgccgagcag cgcgccttcg
     9061 gtatcgattt ttgaaccgac tttaacgggg gaagccgcgt gcagcggcag gctcacggct
     9121 gccaacaaaa ccagtgaacc tgcccagacc tttgagagtg gcatgatgct ttcctcattc
     9181 ttttactgtt gttttcagcg aattaagaga aaagcatagt tgataatggc gggattagcc
     9241 tgagcggacg gattcagttg cagaatcaga taaatactta agaggcatat tcggtccggc
     9301 gtttcctgcc ggatgcggcg cgagcgcctt aaccggccta caaagggcga aaattcagca
     9361 agttaagata tgaacatcag gcaaagggcg caaacgtcgc gcccttgcta aagcattaca
     9421 gcaactcaaa ctcgcctttc ttaacgcgtg cggaatcagt gccgataaag acattgaact
     9481 tgccaggctc ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcgatatcaa
     9541 tcgggaagct gacggtctgg gtttcgcccg gcttcagggt gattttctca aagcctttca
     9601 gctgcttcac tggacggctc atggaagccg tcacatcctg caagtacatc tgcactaccg
     9661 tggccccttc gcgtttaccg gtgttcgtca cctgcacgct ggccgtcact ttgccgtcac
     9721 gcttcatggt cggcgcagaa agtttcacat cagagacggt gaaagtggta tagctcagac
     9781 catagccgaa cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt
     9841 tcggcttgtc ggcattatac ggacgaccgg tgttcagatg gctgtagtac accgggatct
     9901 gcccgacaga acgcgggaag gacatcggca gcttaccgga cgggttgtaa tcgccaaaca
     9961 acacatcggc aattgcatta ccgccttcag tcccggcaaa ccaggtttcc agaatcgcat
    10021 cagcctgctg atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca
    10081 gcggtttgcc ggtggctttc agggcggcaa tcaaatcacg ctggctttgc agaatagtaa
    10141 tatcggtacg gctggaggcc tcgtgcgcca tcccctgtgc ttcaccgact acagccacca
    10201 ccacatcaga ttgtttcgcc gtctgcaccg cttcatcaat catctcttgc ggcgaacgcg
    10261 gatcgacttt gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg
    10321 taacgttcgc ccctttggca tacagcactt taccgttttc accgacggca tttttaatcc
    10381 cggtcagcac ggtcacggat tgatcggcaa cacctgccgc cgaccagctg cccatcacgt
    10441 cacgtttact gtccgccagt ggcccaacca ccgcaatggt ggctgatttt ttcagcggta
    10501 acgtttcgag acggtttttc agcaacacca ggctttcacg cgccacttca cgcgcttctt
    10561 tgcggtgcag gcggctttcg gcattggtat ccaccgggtc agactctttc ggaccgagat
    10621 ggctgtatgg gtcgttaaac aaccccatat catatttaac gttcagtaca tggcgggcag
    10681 catcgtccag ctctgccatc gtcactttac cggatttgat caacccaggc agatacttcg
    10741 agtagtactc gtcgctcatg ctcatgttga ttccggattt cagcgccacg cgcactgcat
    10801 cttccggatc tgccgccgtg ccatgtttaa tcagctcttt gattgcaccg tgatcggaaa
    10861 cggtgatgcc tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggagtcgg
    10921 aggtggctgg cgtgccgttc agcgagttca gcgccaccat caccgcgccg ctgcctgcgt
    10981 ccagccccgc tttgtacggc ggcatataat cattaaacag gcgctgcgga ctcatatcga
    11041 cggtgttgta ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg
    11101 tcatcaccga gtaacgatct gccgggcttt taccctgcat cgcttccacc atggttttac
    11161 ccattattga ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg
    11221 gatcgcgcga gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt
    11281 cataagcaga gacacgtccg accgttttta ctgcatcgag gttaaaagac gaggccagac
    11341 cgaggctaat cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag
    11401 gaattttcag gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg
    11461 ttacggtgtt gaaaatagcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt
    11521 tcgggttatc cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca
    11581 ttttcttaag cagttcggtg acgaacgcat cccgcgcttc aggcgttaat ggatggttac
    11641 cgaataaatc atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac
    11701 atagccattt catatggatt tttcttcctt tgttgcccga cgtggcagcg aaaatggtgc
    11761 aaaaaccgta gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc
    11821 tgaattttca aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat
    11881 gttttttcgc caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc
    11941 ctttttgaat gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa
    12001 aacggcccgc tatcgcaagg gcttccgttc tggtcatggc gacgcgctgg ctgtcgtttt
    12061 ccctggctca ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat
    12121 tattctgatg caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga
    12181 ctatgatcgc gatatcgtga tcatcagcac cctacgtctc gacaagctgc acgttctcgg
    12241 caagggcgaa caggtgctgg cctatccggg caccacgctt tattccctgg aaaaagccct
    12301 caaaccgctg ggacgcgaac cgcactcggt gattggttca tcgtgcattg gcgcatcggt
    12361 tatcggcggt atctgtaata actctggtgg ttcgctggtg cagcgtggcc cggcgtatac
    12421 cgaaatgtcg ttattcgcgc gtataaatga agatggcaaa ctgacgctgg tgaaccatct
    12481 ggggattgat ctgggcgaaa cgccggagca gatccttagc aagctggatg acgatcgcat
    12541 caaagatgac gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg
    12601 cgttcgtgat attgaagccg acacgcccgc acgttataac gccgatccgg atcggttatt
    12661 tgaatcttct ggttgcgccg ggaagctggc cgtctttgca gtacgtcttg ataccttcga
    12721 agcggaaaaa aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga
    12781 aatccgccgt catattctgg ctaacttcga aaatctgccg gttgccgggg aatatatgca
    12841 ccgggatatc tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa
    12901 gttaggcacc gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct
    12961 ggagaaagtg aaattcttcc gtccgcattt tactgaccgt gcgatgcaaa aattcggtca
    13021 cctgttcccc agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca
    13081 tctgctgtta aaaatggcgg gcgatggcgt gggcgaagcc aaatcgtggc tggtggatta
    13141 tttcaaacag gccgaaggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt
    13201 tttacaccgt ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga
    13261 agtcgaagac attctggcgc tggatatcgc tctgcggcgt aacgacaccg agtggtatga
    13321 gcatttaccg ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat
    13381 gtgctatgtc ttccatcagg attacatcgt taaaaaaggc gtggatgtgc atgcgttgaa
    13441 agaacagatg ctggagctgc tacagcagcg cggcgcgcaa taccctgccg aacataacgt
    13501 cggtcatttg tataaagcac cggagacatt gcagaagttt tatcgcgaga acgatccgac
    13561 caacagtatg aatccgggga tcggtaaaac cagtaagcgg aaaaactggc aggaagtgga
    13621 gtaaaaatta cggatggcag agtatcgcca tccgaattca cttaatcgtt ctgtgccgtc
    13681 tgccccgccg ccgccatttg ggcggctttt tgttttttat agcttaacgc tgctgccgga
    13741 acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg
    13801 gtgtatttgc caaacgcatc catcactacc agcgccaccg gtttattatt gataaccgta
    13861 cgcatcacca gacaatggcc cgccgcattg gtaaagccgg ttttggttag ctggatattc
    13921 cagttatcgc gatacaccag atgattagta ttgcggaacg gcagcgtata cgtcgggtta
    13981 gagaaggttg ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atattgtttg
    14041 ctggcaatga gcagtttggt taagtcacgg gcggttgaaa cgttatgcac cgacaatccg
    14101 gtaggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcattgcc
    14161 ttaataaagg ctttgtaacc accgggataa tggtgcgcaa ggcttgccgc cgcgcggttt
    14221 tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct attcagtcgt
    14281 acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt tagtttttca
    14341 tccagcggca gtcgtgcatc cagcacgacc atcgcagtca ttaatttgct gatagacgca
    14401 atcggacgca ccagatccgg gtggttcgaa tagatcactt tgttggtatt cagatcaaca
    14461 atcatcgcgc taccggaggc aatttccggt tgtgaagcgg tggtagcggc tgccgttttc
    14521 gcaacggcct gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact
    14581 cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacgggtt gcgcaccgcc
    14641 ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca
    14701 aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg caacgtcaaa
    14761 gaactttcta gaacaaacga taaccgtagc cccatagtat aacggtaagg gcgagcagca
    14821 cttccagtac cagcacgcca atcgccagcg tcgaactgga gaagctaagg ccttcctctt
    14881 tgttgatatt caggaagctc ggaataccaa ggtaaagtag gtaaccggtg taaaacagtg
    14941 ccaccgtgcc gaccagcgca cacaaccaga ccagcggata aagcgccacc agaccactta
    15001 aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct
    15061 gcggataatt acgcgccatc caccagatga cccgccccat caccgcgacc ccagcgagca
    15121 taacgccata aaacaggaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag
    15181 tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa
    15241 tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg ttttcgcgat
    15301 taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc
    15361 ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt
    15421 ttgttcgccc atgtaatttg caccgtctga tgtacgtcct ttttcagcgt gaatgattca
    15481 cagggtgtat gcttacaggc aaccaaaggg agacagactg gcctatggat ctcaatacac
    15541 tcatctcaca atatggttat gccgcgctgg ttatcggtag cctggcggaa ggtgaaaccg
    15601 tgactttgct gggaggcctt gcggcacatc aggggctatt aaagttcccg ctggtggtac
    15661 tttctgtagc gcttggcggc atgattggcg accaggtgct ctatctgtgc gggcggcggt
    15721 ttggcggtaa gctgttacgc cgtttctcta aacatcagga taaaattgag cgggcgcaga
    15781 aactgatcca acgccatccg tatctgtttg tcattggtac gcgctttatg tatggctttc
    15841 gggtgattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccgc
    15901 ttaatattct cggcgcattt gcctgggcgt tgatttttac cactatcggt tacgctggtg
    15961 gtcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac tgggtctggt
    16021 tgattctggt cgtggttctg gtggtaggcg tgcgctggtg gctgaaacga cgcgggaaga
    16081 aaaagccgga taatcaggcg taaaaccatt gccctggata aggcgtgcgc gccgcatccg
    16141 acatccggtt caccatgcct gatgcggcgc ttgtgcgtct tatcatgcct accaaaattt
    16201 tacccattga aatatccaca cacctcactg cgctattctg ggttgaactg cggattcgcc
    16261 aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc gccctccgaa
    16321 caaagccacg ccaccaggct ggcaatctca tgcgttgcgc caaaacgccg cagaggaatc
    16381 gaaggctccg cgtcgggctt cacgtcgcta tcatccatgc cgttcattgg cgtagcgatc
    16441 gccccaggtg cgactgcgtt caccaaaatc ttatgcctga ccaactccag cgccattgct
    16501 ttggttaacc cgccgagcgc atgtttagcg gctgtgtagg cgctggcatc cggcagcggc
    16561 gtatgttcat gtaccgacgt aatgttgatg atgcgaccac cctgtccttg tttcaccatc
    16621 tggcgggcag caatttgcga gcataaaaat gcaccatcga catcaacggt aaaaatcttg
    16681 cgccactcat cgaaagccat atcaagaaac ggcactttgg tcattgcgcc cgcattattc
    16741 accagcacat caatacgccc cagccgttga ataagtttct ccagcgcctg cgcaccttct
    16801 ggcagattgc cgagatccag ttgcacgatc tccgcacgta cgccgtggct aactacctca
    16861 cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat atcaaaccct
    16921 tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc
    16981 gctacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta tagaagtcac
    17041 atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg aggcatccag
    17101 ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc
    17161 gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgg gcctgcgcaa gacctaccac
    17221 acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt ttctccgcca gtgtctgaac
    17281 ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggc
    17341 gatagacaag ttgctttcgg cttttgcgat atcgagattg gcgttaagac gaccactatc
    17401 gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag
    17461 atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa
    17521 tgccgctttt gccgcatcaa tggtgcttag cgatgactca acgtaccagt gcgccgcctg
    17581 caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct ggcttgccac
    17641 tttcggcaac gccaccggtt taagcgtcaa tgacttagtc tggtgatttg tgagtgcaat
    17701 taaccgtgcc tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt
    17761 gctggcatta atatcggttt ccacaccttc aactgaagaa gtaatcccgt tctgatatag
    17821 ctggcgatcg gtcgcgataa tggtgttctg ctctttttcg atttgctgca agaccgtgtt
    17881 taacgccgcc tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg
    17941 gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc
    18001 cgcccgattc tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagttccgtt
    18061 ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc agagcaaacg gccccattag
    18121 cccttctgcc gacatttttt gccgctccat atccgcagaa aagtcgatct gcggaccatc
    18181 ctgagtagca actgccttcg cctgggcttc agctaactga atgcgctgtt cagccacctg
    18241 catatccggt gcgttctgta gtgcattgtt aattaaggaa gtgagttgat tatcgtgata
    18301 ctccagccac cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc
    18361 aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggcgcaca
    18421 cccggccagc atcagtaaca gcggaaaaca ggcgatggct ggataaaagg aatcacgatt
    18481 catgggggaa taatcaggta agaaaaggtg cgcggagatt accgtgtgtt gcgatatatt
    18541 ttttagtttc gcgtggcaat acatcagtag caataaaacg acatatccag aaaaatatac
    18601 actaagtgaa tgatatcttc cgatttatct taatcgttta tggataacgg caaagggctt
    18661 cgttttttcc tatacttatt cagcactcac aaataaagga acgccaatga aaattatact
    18721 ctgggctgta ttgattattt tcctgattgg gctactggtg gtgactggcg tatttaagat
    18781 gatattttaa aaatcagata tgacatataa gttgtcaggt caggaactag atattttgtg
    18841 tacttgattt gcaaacagct ccggtgttgg tggcgtcaaa atcgctgaag cgttccccga
    18901 aggccggggc agcccacatg gatgtgggct gagggcgcgt cttacaagga cgtttcctcg
    18961 cgcccgaccc ggtagccgaa ggggataagc tgaaggtacc gcgcagcggc gattttgttc
    19021 gccagagccc gggggtgcag ggggaggagg cgattgtcct ccccctgcgc gctccttgcg
    19081 ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa caagatgatg aagctgaata
    19141 agcgattcat tgtttctgat aaactgcgat tccactatta tcgcagctta ccgatatcaa
    19201 ttgcctgaat agcccttgca atatcagggg aattattcaa cacacgaaca tgctgaaata
    19261 attctgttgc ttcatcgtat tctttacgca aataactcaa ccactgttta atccgcgcta
    19321 cgtgatataa cccggtatcg ccctgctttt ccagacgggt atatttttgc agcaaagcaa
    19381 ccacctccgg ccacggcatt cgcggttcgt tatattttac tacccggctc aggttgggaa
    19441 tattgagcgc cccgcgacca atcatcactg agtcgcagcc gctgatcgcc atgcattctt
    19501 gcgcgctctg ccagtcccag atttcgccgt tggcaatcac cggaatattc agccgctggc
    19561 gaatctcgcc aatcgcctgc cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc
    19621 gtccatgcac caccagctcc gtagcaccag cctgttgaac cgcatcggcg atttcaaatt
    19681 tcttctcacc gctgtcccag cccagacgca ctttcacgct gacgggcaaa tgcgccggta
    19741 cagcttcacg catcgctttt gcaccctggt agatgagttc aggatctttg agtaacgtcg
    19801 ccccaccacc gctaccgtta accgttttcg acgggcagcc gcaattgaga tccacgcccc
    19861 aggaacctaa ttccaccgca cgggcggcgt tctctgccag ccattgtggg aactgaccca
    19921 acaactgcac gcgcaccagc gtaccggatg gtgtccggct ggcgttttgt agctcagggc
    19981 aaatgcgatg aaagactttc accggcagca gttgatccac cacgcggacg aactcggtga
    20041 tgcacagatc gtagtcgtta acttcggtca gcaattcacg caccagagag tcaagcactc
    20101 cctccatcgg agccagtaac acacgcatat catcacccgc aaaaaaatga ggcgctatgt
    20161 tagcgcctcc tgtgtttgca gacaaactga cttactgatt aattcgggca agatttttac
    20221 cctcacccta accctctccc gaaggggcga ggggaccgcc cgtgcgcctg attgggtgaa
    20281 gaagtgctta gcgtggttgc aaacagttat cttctttcca gccgtacaac cactccatgc
    20341 cgcgataaaa ttctgcctgc gatttccctt tccacagcag attacgtacc tggcgttcta
    20401 cccctgcggc gtggtaaacc cgccccattt cacgggtcga ccagacgatg cgcgcggtgc
    20461 gcggaattcg taccgactca tacagcgcaa aggcttgcgc cgcatcaccc tcacactgcg
    20521 ccagcgcctt acccagcgtt actgcatctt ccagcgccat acaggctccc tgcgccatat
    20581 attgcgccac cggatgggcc gaatcgccca caagcgttat gcgcttcgtt ccccacttcg
    20641 ccaccggttc acggtcagcg gttgaccagc gtcgccatga agtcggttta tccaacatct
    20701 ggcgcggacg ggggtgaatc ccttcaaagt aagagagcac ttcctcttta ctaccatcct
    20761 tcacgcccca ctcttcctgc tggcggctgt ggaatgttac caccaggtta tactgcttgc
    20821 cgccgcgcaa agggtaatgc accaagtgac aatgcggccc cgcccacagt accggtgcgt
    20881 taatgcgcaa atcttccggc atatcggcac gatcgataac cgcacggtag acaacatgtc
    20941 cggtgacacg cggcgcatcg cccaataagc tctggcgcac cactgacttc acgccgtcac
    21001 agccaatcag aatatcgccg gtccagctat tgccgcgatc gtcaaacacg gtgacatcgt
    21061 cttccgtctc gcgaatatcc acgacgttgg tggaggtgtg atattccacg tccggatgcg
    21121 tcagtaccgc ttcccacacc gaggcgtgaa tatcaacccg gtggatcacc gcgtaagggc
    21181 cgccgaaatg gtcacgaaag gcctgcccgg tttcaatgca caccacttct tcggcgttca
    21241 ccgcatccat catggtaatg tgatcggtaa acaccgcgcg ctggcgggca acttcgccga
    21301 cgccgaggct gtccagcgcc gaaaacgcat ttggccccaa ctggatgccc gcgccaatct
    21361 caccgatctc atgcgccttt tccagcagca ccactttgat ccccaggcgg gccagtgaca
    21421 gcgcagttgc cgcaccgccg atcccgcctc ccacaattac tgcgcgtgtc actttcgtca
    21481 ttatagttct cctggtcagg ctgaaatttt atcttgttga ttttctggcg ctgcggcaat
    21541 gaacgctggc agctgtgtgc aggcgtcgta caccgctttg cagcgtggat acccgctcaa
    21601 atcgcagttc atgcgtagcg cgttcgccca ctgcgggaca aggcagcaat ctgccagcgt
    21661 cggcgtctcg cccacacaaa attgccccga ctggttctgg cgcaacagtt gttccaccgc
    21721 gcttaaccct tgctggatcc agtgcgcata ccaacgtttt ttttcttctt cactgacgtt
    21781 cagttcttcg gtcaggtagc gcagcacgcg caagttattc aatggatgaa tatcacaagc
    21841 gatggcatag acaatttcca gcaccttgtt gcgggccggc tcttcctgcg gcaccagcgg
    21901 aacttgcggg tagtgcctct ccagccagtc aataatcgcc agcgattgtc cgagtgattc
    21961 gccctcgtca gtcagcagcg tcggcaccag cccgaccgga ttcatccgcc gataggccag
    22021 ttcattttgc tgaccaatac ggatattcac cccttcggtc tggtaattaa tgcctttcag
    22081 cgccagcgca atgcgtaccc ggtacgacgc tgaactatta aaaaaactgt acagcttcat
    22141 catgcacctc agacaatctt caccgcgata ggcgttaagc cctcgacgtt gcctgtgatc
    22201 acatcacctt tcactaccgg gccaacccct tccggcgtac cggtgaagat cagatcgccc
    22261 ggttgcagct caaagaagcc tgacagatag cagatggttt cattcaccga ccagatgaga
    22321 tggcgaatat cgctgcgctg atgatcttcg ccgttaacct gtaaccagat tggcgcatta
    22381 tcaataccgc cgatatcgcg ggctttgtgc agcggcgcga tgggcgctga gagatcaaac
    22441 gctttaccga tttcccacgg gcggcccatc tggcgcattt ccatttgacg gtcacgccga
    22501 gtcatatcaa gacccgtggc ataaccccag acatattcat gggcgtgttc cagcgggata
    22561 tcgctgcctt ttttaccaat cgccaccacc agttcaattt cgtaatggta gttatcggtc
    22621 tgtgccggat acggcagcgc cagcgtttca ccagcggcaa ccggaacaac ggcatccgcc
    22681 ggtttgcaga agaagaatgg cggttcacga tccggatcaa agcccatttc gcgggcgtga
    22741 gcggcatagt tacgtccgac gcagtaaacg cggcgcacgg ggaattgttc atcactgcca
    22801 acaaccggta cggtaacggg cgcttgcggt tcgaatacgt attgagtcat ttttctctcc
    22861 taaattaata acgcgcttcg cggaacagcc ccagggcttc ttgtaccggt ctgtccgaaa
    22921 aactaaataa aacggaatct tgcgtggtct ggaacgacac gccatgccag gtcggcacca
    22981 cgaagatatc ttttgcacta aagctgaagg tttcattgcc gatgatgacc tgcccgctac
    23041 cttccaccac atggtagatg gtgctgtcag tggtacgcgc aacgcgtgag gcaaaccctt
    23101 tcggcagcag ttgcaggaaa gcgcccatcg acggcatcgg atagccgccg gtgaccgggt
    23161 tgacgtagcg cattttgtag ccatcccact cgtcggcatc gcccagtcgg gttaaatcgt
    23221 gcagcacttc gcggctgcgg tcataacgat agttaaagat cggcgaggag ttccccgtct
    23281 gatgccgcag cggcaacata ttggcagcgt aacgcggcag ataatctccc tctttacgcg
    23341 ttaccggttg ttgctcttcc ggataatctt cggcaaaacc gcagcccaga atattcacta
    23401 atggcagatc cagcccatcg agccagataa ccggttcgtc gccagggtta ccgtgatcgt
    23461 gccagcgcca ctgcggggtc aggataaaat cgccttcatt cattggcgtg cgttcgccat
    23521 caacggcggt aaatgcccct ttgccttcaa caataaaacg cagcgccgac tgattatgac
    23581 gatgactcgg tgccacttcg cccggcatga tcagttgcaa accggcatat aaagtcgcgg
    23641 taatggaaga ctgcccgcgc agcgccgggt tttccagcac cagcacgcgg cgcacggcct
    23701 ctttcgcacc aatcaggccg ccgctctcca gtagcagcgg gcggatctcc tgataattcc
    23761 agtacgctgg cgcacagtta gcgttgggcg ttttcggcac cagatggtgc agtgactccc
    23821 acagcggggt caggttttgc ccggaaatat gctgataaaa ttgctcgcgg ctattttgat
    23881 tgttatcagt cattgagatg ctccttattc gttcacaacg cctgcgcgag gcagtgagtc
    23941 agggacagag gtcgactggc gaaccacggt aatcagcagg gttaacatca ccgcactgat
    24001 cgccgcaggc acagcgataa tgaaaaacag ggtgtcgaaa gagaaattca tcgccatcat
    24061 cacgccgcca gacagcgaac caacaatcgc gccgcagcgg ccgatagcat ttgaccagct
    24121 cacgcccgtg gctcgacttt gtgttggata aagtgttgcg gtcagcgcat tcagccccac
    24181 ctgcgatccg ctaatgccaa tgccagtgcc gaaaatcgcc agcgccatca accataatcc
    24241 atcctggctt aagccaatca tcacgatgca tatagcgcct atggcatagc tcagcgtcag
    24301 cacgcggaaa ggattgaact tatccatcag cacacccagc gccagcgcgc ctaacgtacc
    24361 accaatctgg aacgcggcgg tgacccagga cgcgtgttgc aggtcgatgc cgcgatggtt
    24421 cagtagcgtc ggcatccagc ttgagagcag gtaaataatc agcaggctca taaagaacac
    24481 tacccacagc atcaacgtga tgggtaactg acgtcccata aacagttgac gaatgctgcc
    24541 ttttgtaacg gaggctgatt caatgagata aaagtgggta tcgacataac gctcgcgggt
    24601 gatggcgctg acggttttcg caatcaccgc ctgcggtagc tggcggcgca cctgccagcg
    24661 tggcgattcc ggcagaacta ccagcagtgc gacaaacagc atcagcggta acacgccccc
    24721 caacaccaga atgccgtgcc agccaatcac cggcaccagc tgcgcactga caatcccgcc
    24781 aaacgccgaa cccagcgtaa agccgcaaaa catcagcgtc accagcgcgc cgcggcggcg
    24841 ggcaggcaga tactccgagg tcatggtgat ggtgttcggc atcgcgccgc ccagacccag
    24901 gccggtgaga aagcgcagga agaccagcgt ttgcagatcc ggtgagaaag ccgaggcgag
    24961 gcttaacgcg ccaaacaaaa agacgcacag ctcaatcacc cgcttgcgac caaagcgatc
    25021 ggacaacggg ccacaaagca gtgcgcctgc ggttaatcct agcagacccg cgccaaaaag
    25081 cggggcgaga tcgcctgcgg tgagttgcca gtgagtgcga atatcagggg cgataaatcc
    25141 gatagccgcg gtgtcaaaac catcgagcat taccaccaga aaacagcaga tgataacgcg
    25201 ccactgcatc ttgccgaccg gagcagcatc gataagtgct tgtaaatcac gtcgttgagt
    25261 catagtaaat gcctcagtgc aggtaaggta gatgtttctt ttgttttgta tatgttgcga
    25321 tattgtgatt aaagagatga gatgtacaat ggcatttcgg gcagagagat aaccttgagg
    25381 ttatggcaaa tggcgaactg ggcgcaaaaa ttaaaactac atcaccttga aatgctggtg
    25441 gcgctgggtg aacagggcaa tctaacccat gtcgcacgaa tgatgaatat cacccaacct
    25501 gcgctttcga aatggttgtc acaactcgaa gatgagattg gaatcacact ttttgagcgg
    25561 cacagcaaag gattacgtcc gactgaaggc ggcaaattgt tattgcaaca tgcccaacgg
    25621 ttaatcaatg acatggagcg atcgcaacat gaactggccc gttttaaaca aggtggactg
    25681 gtcggcagtc tgaaaattgg ctgttctccc gtcgccactg actgcgtatc ccaggcgatt
    25741 ttgcatttgc ttacgaagat gccaactcta cacctgaata tcgaagagaa ggtgatgacc
    25801 ccattgctgc aagatctatt ggccggttct gtcgatgtgg tcgttgggcg aattggcggt
    25861 cgcgcattgc agctgccact gaattatcag gtgctgtaca ccgagccagt atgctttgtc
    25921 gcccgccctg agcacccact ggcgaaatac gccaccctca gctggaatga tcttgccaac
    25981 tggcgctgga tcgtctggcc gacgggtacc cccattcgca ttagtattga taatgcgctg
    26041 gtagaccacg gcgtaatgct gccagaaaac acgattgagt cggcgtcgat gaacgtcagt
    26101 accaacctgt tacaaagcag cgatatgatc tctattttgt cattacgcct ggcgcaacgt
    26161 tatgccagcc agggacaact ggtgatactc aacctgccaa agatagaaca gaaaggtagc
    26221 gtcggtgtgt tctggcgcaa taacgaaacg ccttcttatg ctttaagtca gtttctgcac
    26281 tttttacagc agaattagtg tgctgaacgg ggcgctcgcc cgaggttacc tccctaaaat
    26341 tcaactatgc tgagaattct taatgtgatc ggtagcacgt tttacgaatt aattgtatga
    26401 tgaatccatc tcatctgggg tgttgattat gagtaagaca ctgaacatta tctggcaata
    26461 tttacgcgct ttcgtcctga tttatgcctg cctgtatgca ggcattttca ttgcttccct
    26521 gctaccggta accattccgg gcagcatcat cgggatgctg atcctgtttg tcctgctggc
    26581 cttgcaaatt cttccggcga aatgggtcaa tccggggtgc tacgtactga ttcgctatat
    26641 ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt tgctccgcgc
    26701 acagtttggc ccggtagtgg tttcctgtgc aatcagtacg ctggtggttt ttctggtggt
    26761 gagctggagt tcgcaactgg tacacggtga acgtaaagtc gtaggtcaga aaggatcaga
    26821 agaatgatgg cgaatatctg gtggtcatta ccgctgacgt tgattgtctt ttttgccgcc
    26881 cgcaaactgg cggcacggta taagtttccc ctgcttaacc cgttgctggt agcaatggtg
    26941 gtgatcattc cttttttaat gctgaccggc atctcttacg acagttactt taaaggtagc
    27001 gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc tttatatgag
    27061 cagctacacc agatccgcgc gcgctggaaa tcgatcatca ccatctgttt tatcggcagc
    27121 gtggttgcga tggtgacggg gacttccgtg gcattgttga tgggcgcttc accggaaatc
    27181 gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg caatggcggt tggcggcagt
    27241 attggcggta ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat cctcggcgcg
    27301 gtatttggcc atacattgct taatgcgatg cgtattcgta ccaaagctgc gcgcggtctg
    27361 gcaatgggga ctgcctcgca cgccctcggt acggcgcgct gcgccgagct ggattatcag
    27421 gaaggtgcat ttagttcgct agcgctggtg ttatgcggga taattacttc gctgatcgca
    27481 ccgatccttt tcccgattat tctggcagta atgggctaaa atttgcgatg cgtcgcgcat
    27541 ttttgatgta agtttcacgc gttgcataat taatgagatt cagatcacat ataaagccat
    27601 aacgggttcg taaactgtta tcccattaca tgattatgag gcaacgccat gcatccacgt
    27661 tttcaaaccg cttttgccca acttgcggat aacttgcaat ctgcactgga acctattctg
    27721 gcagacaagt acttccccgc tttgttgacc ggggagcaag tctcatcgct gaagagcgca
    27781 acggggctgg acgaagacgc gctggcattc gcactacttc cgctggcggc ggcctgtgcg
    27841 cgtacgccat tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag cggaacctgg
    27901 tattttggtg ccaatatgga atttattggt gcgacaatgc agcaaaccgt tcatgccgaa
    27961 caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc catcaccgtt
    28021 aactacacgc cttgtggtca ctgccgtcag tttatgaatg aactgaacag cggtctggat
    28081 ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc gtgactatct ggcggatgcc
    28141 tttgggccga aagatctgga gattaaaacg ctgctgatgg acgagcagga tcacggctat
    28201 gcgctgactg gtgatgcgct ttctcaggcg gcgattgcgg cggcaaaccg ttcgcacatg
    28261 ccttacagta agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg tattttcagc
    28321 ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc caccgttgca gggagcgtta
    28381 attctattga atctcaaggg ttatgattac ccggatatcc agcgcgcagt tctggcagag
    28441 aaagccgatg cgccgttgat tcagtgggat gccacctccg caacactgaa agctctcggc
    28501 tgtcacaata tcgaccgcgt gcttctcgct taagcctggt gccggatgcg gcgtgaacgc
    28561 cttgtccggc ttgccagccc tctcctggtg tcgaaaatcc cggcaaacag tttgtcgttt
    28621 cttgcgcaaa accagcgggt aaagtagcct gatggaaatt ttccttagat cgagtctcct
    28681 gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg ctgttgactg
    28741 tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc ttatatctac gacgaattgc
    28801 aggatctccc ctaccgccag gtcggtgtgg tgctcggaac agcaaaatat tatcgtactg
    28861 gcgtaattaa tcagtattat cgctaccgca ttcaaggagc gattaatgcc tataacagcg
    28921 gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt gcaaagttat aatgagccga
    28981 tgaccatgcg caaagattta atcgctgctg gtgtcgaccc atcagatatt gttctcgatt
    29041 acgcaggctt tcgtacgctg gattccatcg tgcgtacacg caaagttttc gatactaatg
    29101 atttcattat tatcacccaa cgtttccact gtgagcgggc attatttatt gcgctgcata
    29161 tggggattca ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg tcagtacgta
    29221 ttcgtgaatt tgccgcccgt ttcggtgcgc tggctgacct ttatattttt aaacgtgaac
    29281 cgcgtttttt agggccgctg gtccctattc cggctatgca ccaggtgccg gaagatgcgc
    29341 aggggtatcc cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa caaggaaagt
    29401 agttatggat gtacagcagt tttttgtcgt tgccgttttt ttccttatcc cgatattttg
    29461 tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt gataaacggg ttaaaaatgc
    29521 accggaaccg gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg cttatatggt
    29581 ggcatatatc ggcttcggaa ttttatctat cggcatgatt gtttatctta ttttctatcg
    29641 ttaatatttt ctcttttctg ttaattattg agaataatta ttacttcact caataagctg
    29701 cggatataac attctgaacc gcagctattt gtgaagcctt tcacagttta aatttcccca
    29761 cgcgcatagc cttaatatca gcacgcaata tttatttagc gttcgcctta attactcata
    29821 gcattaagga agatcacatg ccgcaacaaa attatctgga tgaactcact ccggctttta
    29881 cgccgttact ggcgattaaa gaagcctctc gctgtttatt atgtcacgac gctccctgta
    29941 gtcaggcttg cccggcacag accgatccgg ggaaatttat tcgctcaatc tactttcgta
    30001 attttaaagg cgctgccgag acaattcgcg aaaataatgc cctcggtgcc gtttgtgcca
    30061 gagtgtgccc gacggagaaa ttatgccaaa gcggttgtac ccgtgccggt gttgatgctc
    30121 ccattgatat cggccgctta cagcgttttg ttactgattt tgaacaacaa accgggatgg
    30181 aaatttatca gcccggtact aaaacgctcg gcaaagtcgc gattattggc gcaggtcctg
    30241 ccggattgca ggccagtgtg acgctaacaa accagggtta tgacgtcacg atttatgaga
    30301 aagaagcgca ccccggtggc tggttgcgta acggtattcc gcaatttcgg ttaccgcaat
    30361 cagtgctgga tgcagaaatc gcccgtattg aaaaaatggg cgtgaccatt aagtgcaata
    30421 acgaaatcgg taaaacactc accctggagc aactgaaagc ggaaaaccgc gcggtactgg
    30481 tcaccgtggg gttatcaagc ggttccgggc taccgctgtt tgagcatagc gacgttgaga
    30541 ttgccgtcga cttcctgcta cgtgcacgac aggcgcaagg cgatatcagc attccacaaa
    30601 gcgcattaat tatcggcggc ggtgatgtcg cgatggacgt agccagcacg ctgaaagttc
    30661 tcggctgtca ggcggtaact tgcgtagcgc gtgaagagtt agatgagttt ccggcaagcg
    30721 aaaaagagtt tgccagcgcc cgggagctgg gcgtttcgat catcgatgga ttcacgccag
    30781 tagccgtgga aggcaataaa gtcacgttta agcatgtacg gttatcgggc gaactgacga
    30841 tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc cagactggat gactttgcga
    30901 agttagagcc gcagcgtaac accatcaaaa cacaaaatta tcagacccgc gatccgcaag
    30961 tctttgctgc tggcgatatt gttgagggtg acaaaaccgt ggtctatgcc gtgaaaaccg
    31021 ggaaagaagc cgccgaggcg attcatcact atttagaggg agcttgctca tgttaacgaa
    31081 agatctttcg attacttttt gcggcgtgaa gtttcccaac ccgttctgcc tctcttcttc
    31141 gccggtaggc aactgctatg agatgtgtgc caaagcctac gacacaggtt ggggcggcgt
    31201 ggtgtttaaa acgatcggct tttttatcgc caacgaagtc tcgccgcgtt ttgatcatct
    31261 ggtgaaagaa gataccggtt ttatcggctt caaaaatatg gaacagattg ctgaacatcc
    31321 gttggaagag aatctggccg ccctgcgtcg gctgaaggaa gattacccgg acaaagtatt
    31381 gatcgcttcg atcatggggg aaaatgagca gcaatgggag gagctggcgc gcctggtgca
    31441 agaagctggc gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga cttctcatgc
    31501 gatgggtagc gatgtcgggc aaagcccgga gctggtagaa aaatattgtc gggcagtgaa
    31561 acggggttcc acgctgccaa tgctggcgaa gatgacgccg aatatcggtg atatgtgcga
    31621 agtggcgctg gcggcgaagc gcggcggcgc agatggcatt gcggcgatta acaccgttaa
    31681 atccatcacc aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta acggaaaatc
    31741 gagtatttcc ggatattccg gtaaagcggt aaaaccgatc gccctgcgct tcattcagca
    31801 aatgcgcacc catccagaac tgcgcgattt cccaatcagc ggtatcggcg gcattgaaac
    31861 ctgggaggat gcggcggagt ttttattgct cggcgcagca acgttacagg tcaccaccgg
    31921 cattatgcag tacggctatc gcatcgtaga agatatggcg agcgggttat cgcattatct
    31981 cgccgatcag ggatttgatt cgttgcagga gatggtaggt ctggcgaata acaatattgt
    32041 cccggcggaa gatttagacc gcagttatat tgtctatccc cgtatcaatc ttgataaatg
    32101 tgttggctgt ggacgctgtt atatttcctg ttacgacggc ggtcaccagg cgatggaatg
    32161 gagcgagaaa acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt gtctgctttg
    32221 tggtcacgtc tgcccggtgg gttgtattga gctcggggaa gtgaagttta agaaaggcga
    32281 gaaagaacac ccggtaacgt tgtaaaacca gagacgcatc cggcatttgg ttcctgtcgc
    32341 cggatgcggc ttgaacgcct tatccggcct acaaaaccat acaaatcagt atgttgagct
    32401 cattgcaggc cggataaaac gcgccagcgt tgcatccggc atttggctcc tgtcgccgga
    32461 tgcggcgtga acgccttatc cggcctacaa aaccacacag atcatgatgt tatgtgcgtt
    32521 tatgaccgaa tgcggaccac aatcatttca cttcttacgc gcgtacttca gtgaatccag
    32581 cgctacggcg aagataataa tcgccccttt gatgatgtac tgccagtatg ggtttacgcc
    32641 gatgtacgtc aggccatagt tgatgacggt aaaaataatt accccggtca ccacgccaat
    32701 caccgtcccc acaccgccgc tgaacgatac accgcctacc acgcacgccg cgatagcatc
    32761 cagttcatac ataaagccga ggttgttggt ggcagagccg atacgtccgg cttctaacat
    32821 cccgccaaag gcatagaaca cgccagacaa cgcgtagatc atcagcaggt tcaggccgac
    32881 gttgacacca gatacttttg ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc
    32941 gaagcgggtt ttgttccaca acacccagac gaacgccacc gcaatcaacg cgtagaaggt
    33001 gatgtaagag agacggaaac tccccagcgc gacaaagccc tgagcaaagg tagagaagcc
    33061 actgtcaaaa ccagaaattg gcgacgcccc gacaaagtcg taataaagcg agttgatgcc
    33121 atagacgatg atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga gataagcgat
    33181 aatcagaccg ttgatcaaac cgatcaccgc accaatggca cagacaatca gaataaccag
    33241 cgcaatcggc atcgtcgcca tttccgggaa cactttgttg gcgttatcca tggactgcaa
    33301 taatgtcgct gccaccactg ccgccagccc tacttgacgc cccgcagaaa ggtcggtccc
    33361 ctgggtgaca attaaccctg ccacaccgag cgcgataata atacgcaccg atgactgggt
    33421 gagaatatta cttaagttca acagacttaa aaatgttggg gcctggaaaa taataatcgc
    33481 aagcaaaact aaaagaacga cgtaaatacc gccctctttc aggtaagtaa gaaaactttt
    33541 cttatttaac gcactcatgg gaagccccta atcttaaagg tgcaaagacg caagacgtag
    33601 aatttcgttt tgcgttgttg tttttgtatc gacaattccg gaaacgaggc cattgctcat
    33661 aaccagaata cggtctgtta tccctaacaa ctctggcatt tcagaggaga taataataat
    33721 ccccttgcct ttcttcgcca gttcggcaat taactgataa atctcaaact tcgccccaac
    33781 atcaataccg cgcgtcggtt catcgagcat taatatttct ggttgcgtta acagccagcg
    33841 cccaataatc accttttgct gattaccacc tgagagcgaa ccaatttgcg tccgatgccc
    33901 cggtgttttt acccgcatcg aatcaatcac ccactgggta tcgcttttca tccgcgagtt
    33961 atccagtaaa ccaactttat ttttgtagtt gcgaatattg gaaattaacg agttaaaacc
    34021 aatatccaga taggcataaa ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc
    34081 gtggtttatg gcttcgttgg cattatggtt attgatcttt ttgccgtgca acgtaatggt
    34141 gccagccgat ttctcgcgaa taccaaataa cgtctcaaca atatcggtac gtttcgcccc
    34201 caccagaccg gcaataccga ggatctcccc tttatgcaga tcaaacgaga catcgcgaat
    34261 cgacggctgg cgcagtgacg tcaggttacg tacctcgagg atgacttcgc ccggcttgtt
    34321 ttctttgtca ggaaaacgct ggttaagaga acgcccaacc atcatggcga taatcttgtc
    34381 catcgtcagc cctgccagcg gttcggtggc gatccactga ccgtcgcgca atacggtaac
    34441 ttcatcacat aactggaaga tttcctccat tttatgcgag atataaacaa taccgcagcc
    34501 gcgctctttt aatttacgaa taatagtgaa cagatgattg acctcttttt cggttaacga
    34561 agaagtcggt tcatccataa tcacaatttt cgcgttatag gaaaacgctt tggcgatttc
    34621 gatcatctgc atttgcgaaa cagataatgt gccgacgcgc gcacgcggat cgatatcaat
    34681 atccagttca tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat cgacaaacat
    34741 gcctttggtg gggtatcgcc caagccacat gttgtccatc accgaacgtt gtaataccag
    34801 gtttaactcc tggtgtacca tcgaaatacc attttccagt gcttctttgg ccgaatggaa
    34861 atcgatctct ttaccctgga ataaaatggt gccggagtct tttttataaa taccaaacag
    34921 gcattttaat aatgttgatt ttcctgcgcc gttttccccc attaatgcat ggatagaatg
    34981 tggccggact tttaaattaa cgttatcaag tgccttaaca ccaggaaaag acttgttgat
    35041 accgctcatt tccaacaagt attccccgga agacggagtc gttgagctga ccatataatt
    35101 ttaccttgtt ggccatacaa taagggcgca gtaatagact gcgcccaatc agtcttattt
    35161 cttgctgaat tcagccaggt tgtctttatc tacgccaaca taaggtacgc ggaccacttt
    35221 gttgtcgatt ttccagttgg tgccatcagc cgcaccttta ccatcggcca ggtttttcgc
    35281 cagatcaaag gtcgctttcg cctggttgtt agcatcgttc agtacggtgc ccgccagtgc
    35341 accggatttc accagcgcca gcgcttctgg cagagcatcg acgccaaaca ccggaatgct
    35401 ggacttgttg tgtgctttca gtgcttctac cgcgcccatt gccatcgcat cgttgttggc
    35461 gataaccact tcgattttgt tggcgttcgg gccagacagc caggcgtcca tcttatcttt
    35521 cgcctgagcg gtatcccaca tagcggtatc taactgtaac tgttcagttt taatgccttt
    35581 gtcgttcagc tctttaatca cgtaagtggt acgtgcttct gcatccggat ggcccggttc
    35641 acctttcagc agtacgaatt gaatctgacc gtctttattc agatcccaac cctgattcgc
    35701 cgcccagtgt ttagcaatca aatcgccttg aataatgccg gactctttgg agtcagtgcc
    35761 aacgtagtag gctttgtcgt agctatccag cgccttacga gacggttctt tgttaaagaa
    35821 taccaccggc acattttgcc cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc
    35881 aaccaggttg attgccagtg ccttcacccc tttcgccagc aatacgtcga tctgatcgtt
    35941 ctgcttggac tggtcattct gtgaatcatt catcagcagc tgaacatctg gcgcggcttt
    36001 cgcatcttgc tcaatagcct tgcgcactac agacataaag ttatcgtcgt acttatagat
    36061 tgttacacca atgcgagtat cagcagcgtg tgcagtggca ccgaataaca tgctggccat
    36121 cacagcagac agggttaaca ccttcttatt catggtatct ccggtttttc ttatgcaggg
    36181 tagtgcttga gataaatgct cggcggggca gtagagttaa tgaagtgtta ctgaacgccg
    36241 aagctcactt tttaaaattc gttcttccat gcccggtaac gctccagaaa acggctttaa
    36301 ttgttgttat gacgcattac ctcggtgaaa gtgattattc actgttacat acgggttaca
    36361 acgttaaaac ggtgcaatca tagctatcac attgttaaga tactgtgaaa tcactcacag
    36421 attgaaagcg gttacatcgc ctgatttgtt gagttagtga tcgccgccgc attctggcgt
    36481 gttgcgacag aatggcgacg cactaacgtc ggcataaaac agtggctggc acgaggatca
    36541 atattgcctg ctgccccctg caaggccagt tcggtggcta atttcgccat tgaagcaatg
    36601 ggataacgca cggtcgttaa ttgcgggtcg gtgtaacggg cgatgggaat atcatcgaaa
    36661 ccgatgattg agagatgtaa cggaatcgca atgccattat cttttaatgc tgtcagtgcg
    36721 ccagcagcca tattgtcgtt ataagcaaat acagcggtaa gttgtagatt gcgccccagc
    36781 agttcaacca tcgccgcctc accgcccggc atgtccggcg taccagtgcc aatccagctt
    36841 tccggcggaa taatatcctg ctctttcaac gcactcatcc agcctgcttt acgcatggcg
    36901 tcatcttcaa tgccgtggct ggaagaaaga taaccaatac gttgatgacc gttattcagc
    36961 aacatgcgcg tcgccattcg ggcaccgctg agattatcca ggcaaacgca acgatgggtg
    37021 taccccggca caacgcggtt gattaacacc ataccgggaa tgttatccat aaattgcgcc
    37081 agttcatcgt cactcaatgc ttttgagtga acaatcaacg cattacaacg ctggcgaatt
    37141 aacacctcaa tggcgtgacg ctctttttcc gcttcatgat agctattgcc gattagcacg
    37201 tatttctgat gctgctgagc gaccagatcc accgctttta ccagcgcgcc gaaaaacgca
    37261 tcagaaacgt ccatcaccac cacgccaatg gtgtcgctaa cctgagttgc cagcgcctgg
    37321 gcattggcgt ttggccgata atccagctca ctcacggctt tcattactgc ttcacgcgtg
    37381 tcggcactga ccagcgtgct gttattgagc acccgggaaa ccgttgccac agagacgcca
    37441 gcctgacgcg ctacatcacg aatggtgatc atattcacta cctgttatga gattgcagta
    37501 aatgactgct tgctgccggc tattttgtca gcactgaata caggacttcg tgaatcgagt
    37561 cacagcaatg gaaacggtta cagccgtttt gttaacgaat gtgatccaga tcgttatctt
    37621 gatgttttag atattgccgg tccggcagca cgcaaagtta actgacgcca gagccattcc
    37681 accggcccct ggcggaagta acgcagccag ataacagaga agagaatatt cgccagccat
    37741 accggaataa caaacgccag caattccaga cggtcaaact gcataaacag accgaggtgg
    37801 taaaaaagcg tggtacaaat cagcgtttgc aatagatagt tggtcagcgc catccgtccg
    37861 acgcaggcga tcgccagcac cagcttaaag cggctcaatt gcggccagaa gccataaaac
    37921 agcgacgcat agccaatcgc ctgaaacggc gcactcagtt cccgcggcat ttgaagtaag
    37981 aaggcacacc agcgatacgc ccagtccagc tgccattgca gggcgatggc aggaaggtta
    38041 atgatcaccc caatcgccac tagcacaaaa ccagtacgac gatagtgacg taagctgaac
    38101 tgccctttca gccagccact gcgcatcaat gcggcaccaa tgagcatcat cccagccagt
    38161 tgccagccat actgtgcgcc cagtgccaat aaactgttcc caacaccatc ggcacgatta
    38221 ctgatcgctt caacgccgcc gtgaagcttc cagtattttt catataaaat agccgatgca
    38281 tccggcgtcc aggcgcggct agtctggcta tcggaaatca accccaataa cagcaaaacg
    38341 ccaagcccca ccagataaag catgacgccg gtattaaaaa ggctttttac cgatggcgca
    38401 tcgcgcacca gccgccagca gattaagccc accagcccgt aagccagcag aatatcgccg
    38461 tcccagaaca ataaaccgtg aataaagccc agcaagacta acagcgttaa ccgcgactgg
    38521 atccagcgtc tgccacgggg cagcaacatt tgcaggccag caccaaacag cagcgcaaaa
    38581 agcgtgagga atttcacctg gccgatgaga tcgagaaatg cccaggtcca tgcatcctgc
    38641 ggcgtaatag cgccgtacca ggcgggatta agataagccg cctttggtag cccaaaggcg
    38701 ctgatgttta atagcaggat ccccagaatg gcaacgccgc gaacaaagtc gagcgtgacg
    38761 ttgcgctcca tggttcctgc ctttaaatca gttgtgatga cgcacagcgc gcagaaactc
    38821 gtggcgcgta ttctgactgg atttgaacaa tccaccaaga gaggtcgttg tcgtggcact
    38881 ggttgcatcg cggatgccac gcgccttcac gcaataatgc accgcgtcga tagagacagc
    38941 cacgttattg gtgcccagca gcgtttgtag cgcaatgaga atttgctgcg tcagacgttc
    39001 ctgcacctgc ggacgctggg caaagaactg cacaatacgg ttaatttttg acagaccgat
    39061 caccgaatct ttcgggatat aagccaccgt cgctttgcca tcgatggtaa caaaatggtg
    39121 ttcacaggta ctggtcagag tgatatcgcg cacggtgacc atttcatcga ccttcatttt
    39181 gttttcaatg agggtgattt tcgggaagtt ggcgtaatcc agaccggaga aaatttcatc
    39241 gacatacatt ttagcgatgc gatgcggcgt ttccatcaaa ctgtcatcag ccaggtcgag
    39301 attcagcagc tgcatgattt cggtcatatg accagcaata aggcttttgc gcgtttcgtt
    39361 atccatttca tgcacgggcg ggcgcagcgg tgtttccagt cctcgcgcaa ctaacgcttc
    39421 atgaaccagg gccgcttctt tactgagtga tggcatttat gatttctcct gcaggtgtga
    39481 cgcctccgcc ctgcgtgggg gcaaagttat taagctgatt tacagcctga ttattgtgcg
    39541 tgaggcggcg cacataatcc agtattcaca gcgataatta ttgtaattgc cgctgccttt
    39601 catcagcaga tgttaaaaca tcgttatgca aatacggaag tgaaagttac tcacagcaca
    39661 ttgaataaac ggtatgatga agaaattgca aacaacacaa caaggagcca cgcatggaaa
    39721 tgctcgaaga gcaccgctgt tttgaaggct ggcagcaacg ctggcgacac gactccagta
    39781 ccttaaactg cccgatgacg ttcagtatct ttctcccacc ccctcgtgat cacaccccgc
    39841 caccagtgct gtactggctt tccggattaa cctgcaatga cgagaacttc accaccaagg
    39901 cgggtgccca gcgcgtggcg gcggaactgg ggattgtact ggttatgcca gacaccagcc
    39961 cgcgcggcga aaaggttgcc aacgacgatg gctacgattt aggccagggc gcaggctttt
    40021 atcttaatgc cacgcaaccg ccgtgggcga cgcattaccg gatgtatgat tatctgcgtg
    40081 atgaattacc ggcgctggtt cagtcgcaat ttaatgtcag cgaccgctgc gctattagcg
    40141 gtcattcaat gggtggtcac ggtgcgctga ttatggcgct gaaaaacccg ggcaaataca
    40201 ccagcgtttc ggcctttgcg ccaattgtga atccgtgcag cgtcccgtgg ggaatcaaag
    40261 cgtttagccg ctatttaggt gaggacaaaa atgcatggct ggaatgggac agttgcgcgc
    40321 tgatgtatgc cagtaacgcg caggatgcga tcccgacgct tatcgatcag ggtgataacg
    40381 atcagtttct tgctgaccaa ctgcaacctg cagtacttgc agaagccgcg cgccagaaag
    40441 catggccgat gacgctgcgt attcagccgg gatatgatca cagttactac ttcatcgcct
    40501 cttttataga ggatcacctg cgcttccatg cgcagtattt actgaagtga aagtccgccc
    40561 ggttcgccgg gcatcttctc atcagaagcg ataatccact gccataaagt aacgacgtcc
    40621 gtcttcgtta tagctgtagt cgtcacgact gagatccttg tcgccaaggt tcagcacgcc
    40681 tgcgcgcagt ttgacgtctt tagtcacctg ccaggccgcg ccggtattcc agatggtata
    40741 accgcccggt gttttagccg tcgcgctgtc ggctcgtttc tgcccggtat agtgaccaga
    40801 aacatagaat gaccagtctt ccagcgccag cggtttccag tccagcgtac cgttagcggt
    40861 atggaacggc agatcggata gcggtttgtt ttcgccgttg ctgacatcac gaccatcgtt
    40921 gtaggtgtag ttgagtgaca gtttccattc atcgttgaac ggaattttca gctcggtttc
    40981 cacgccctga atacgtgctt tgttaacgtt gtagtagcta aataccggca caccgttcgc
    41041 tgtaaaacca acaaagtttt ggtagcccgg tgcagcattg acgtcagacg tacggctgat
    41101 gctgatacga tctttcacat cgttacggaa aacggtaacg ctggattcaa ccccttccag
    41161 ccagccttct tcacccatgt agtaaagccc cagctcccaa ctttcgctgg tttctggttt
    41221 cagatccggg ctacccacaa tcttacatgc gccacggcag gaattgctcg tccagtcagg
    41281 gctaagttgc aacagagaag gtgctttaaa tgccgtcgcc cagccccctt tcaccgttac
    41341 ggtgtcggtg gcgttataaa ccaggtaggc acgcggactc cagtgttcac cgtaggtttc
    41401 gtgatcgtcc atacgcacgc cggtcgtcag cgccagcggc tcgaagatcc gccattcatc
    41461 ttccacaaac agcgcgtact ggctggcaga cgttttggag ctggttcccc cggtcaggtt
    41521 caccgcatcg ctaagtttgt cgtgacgcca ttcaccgcca accgtgagaa actgattaat
    41581 cgccgtcagc ggcaacgtgt atttgccgtc gaccgtattg ctttcggaag ttatcgggct
    41641 gctgttgcca gggtttttgt tctcgacttt ctcaccgtag tatttcagtt cgctggtgcc
    41701 gtaatcccaa cgcccattat ggctgacgga gtagttctgg cgttccaggc ggtttttgtc
    41761 cagcgagtcg gaatcacgat cctgacggtc gaaaccgtat ccggcagtaa aatcgtgatt
    41821 ttgattcggt gtccaggcaa attcgacatt gccgtcgcgg ctggagaatc cttcaatacg
    41881 cggcgtttct ccggtatcgg tggtcgttga gttttgcggg tcatcctttt cacgttttgc
    41941 caggctgccg taagctttca ttcccagcac accatcaatt aatggtccac tggtaaagaa
    42001 ctgaccgtta taggtatcac cgcgatcgcg atgttcctga atggtggtat cgacggtaac
    42061 ggtacccgac catttctgac cgattttttt ggtgatgata ttcactacac cgccgagcgc
    42121 atcggaaccg tacagcgacg acatcgggcc acggaccact tcaatacgtt cgatggaatc
    42181 gaccgggatc cagttcagat cgaaatcatt gtggcggaag acggcattgc gggagttaac
    42241 gcgtttaccg tcgacaagaa tcagggtgta gctgctgtcc agaccacgaa tactaacgcc
    42301 cttacggtta tccccttcgt tcgtcagttg tacgccaggc acttctttga ggacatcctt
    42361 cagattctgt accggttttc gctgcaggtc ttcctgggta atgacgctga tactggcggg
    42421 tgcatcttta aggttttgtt caacggaaga tgcagtgaca accatcgttt cgccatcgtc
    42481 atcgaccgct aacacaggcc atgcacaaga aatagcggac aaacacagcc cgacccgtac
    42541 gaaagggttc aacctaaaca ttccatatct ccatgaggta actacgaaaa taaaatgggt
    42601 tatcgctcac atcttcttca cgtccccttg cgtacggcag catcgcggtg gacttatttg
    42661 tatgcaggtg atcatcccag aaagccggac cagcctctga tttgtcagcc ttttttgatt
    42721 gcggcgtaac gataatgcaa acgataacaa ttatcaatcc aaatgttaaa ttttatatcc
    42781 gcatgatttg tcagggaaat ggaattgaaa aagccctctc ggttgagagg gcttagcaag
    42841 gaagggagaa acttatttct tatcgttctg cgggaacttc atttcgctgt agcgtacgaa
    42901 gtgggttcct ttaatcagct tgtagccgaa ccaaataatc aggaacagcg ggataccaat
    42961 atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt cgtagttctg
    43021 gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccaatg ggaagaaacc
    43081 tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtaaccgc gacggaagcg
    43141 atagtggcta atggcaatcc ccagccaggc gataaaaccg gtcatcccgg aggtattcag
    43201 cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca gaccggcaat
    43261 caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat
    43321 gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc
    43381 ggagttaccc gctgacagca ccgccgtcag aataactgcg ttcatcaccg ccgcagcaga
    43441 gagcagaccc gcgtgctgga acaccagggt gaacggacta acgctgatgt ctttaacatc
    43501 gttacgcagc aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa
    43561 cacatagaac aacaggattc gccagaacac ctgacgtacc gcgcgtggaa tgtttttccc
    43621 cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc
    43681 gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat
    43741 tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac
    43801 gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc
    43861 ttcaccaaag ccgcgaacag agatgtagtt cagcaggaag ataacgccga ggaacaacgc
    43921 actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct gagctgcaac
    43981 caggtcaacg gcaatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc
    44041 aaagccctct tcaacatagt tctgaccgta agtggcaaac gaaccggaaa ccggcatata
    44101 tgcagccagt tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcaacatata
    44161 cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag
    44221 acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct ttaattcacg
    44281 gcgtaagccc ggcgcttctg tggttttagt ttcggaaacc atacaaaaat gctatccatc
    44341 taaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt
    44401 tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg
    44461 cgaatgcatg ctgaaggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac
    44521 agcaacttaa cgcggcacat ttgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt
    44581 ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg
    44641 gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcacac gtcgcgacag
    44701 gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc
    44761 catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt
    44821 gccggaaccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta aggtgaccgg
    44881 accacgggcc aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg
    44941 ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat caacgcggaa
    45001 atccagcacc gcttgcatca cgtcctggct attcccaacg ctaagttcaa tcggcaactg
    45061 cggataatca tggcgataac gggcgataac tgcaggcaga atgtagttac cgatggtact
    45121 actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc
    45181 ctgttcaagc aatgccaacg cacgcggata gagcagccgc ccgtgttcat taacaaccag
    45241 tcttttcccc acgcgatcaa acagttgcac gccaagctgc ccttccaggt cggtcaaggc
    45301 tgcgctcact gctgattgcg acaacgccag catcaccgac gcttgggtgg ttgatccact
    45361 tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgcttacca
    45421 cttataaaga ttaattataa atatataatc aattttattt ttaaaccagt tagtcgtaac
    45481 cttatgcccg gttaaaggag agggttatga cgaatatcac cttacagaaa caacatcgta
    45541 cactgtggca ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt
    45601 ggggtggttc cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct
    45661 tgttggggat ggttttaggc aacaccatct atccgcacat ctggaaaagc tgtgacggtg
    45721 gtgtgctgtt tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc
    45781 tgacgttctc gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgctgacgc
    45841 tgtccagtac cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc
    45901 acaccagctg gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga
    45961 ctgagccggt agtgaaagcg gaagccagta aagtaaccgt ggctgttgca accgttgtta
    46021 tcttcgggac cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt
    46081 ttagtccgga aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg
    46141 tggcggcagg tcatgccatc agcccggatg cggaaaacgc agcagttatt tccaaaatgc
    46201 tgcgcgtgat gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt
    46261 ctggggcgaa cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atcttgttca
    46321 tcgtagttgc catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg
    46381 taacgctgga taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg
    46441 tcagcgcgct gaaaaaagct ggggcgaaac ctctgctgat ggcactggta ctgtttgcct
    46501 ggctgattgt tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca
    46561 ctacatcttg ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg
    46621 agtcctcgca tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc
    46681 gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt
    46741 cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa
    46801 aaatatcact acacatcggc gcaaattctt ccccacgaca gttatctgat taacctcgga
    46861 catccggtca ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt
    46921 tgcgaacagc tggggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt
    46981 tcagaagagg attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact
    47041 caaggtgtga cagcggtgat agaaaacacc gccggtcagg gcagtaactt agggtttaaa
    47101 ttcgaacatc tcgcggcgat tatcgacggc gtggaagata aatcccgcgt cggcgtctgc
    47161 attgatacct gccatgcttt cgctgccggg tatgatttgc gtactccagc cgaatgcgag
    47221 aaaacattcg cggattttgc ccgtactgtc ggctttaagt atctgcgcgg gatgcacctt
    47281 aacgatgcga aaagcacctt tggcagccgc gttgaccgcc atcatagcct cggtgaaggc
    47341 aatatcggtc atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg
    47401 ctgatcctcg aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg
    47461 caacaaactg aaaaagcggt agcctgaaga tgaataaccg ggaaaaggag atccttgcaa
    47521 ttttacggcg taacccgctg attcagcaga acgaaattgc ggacatgctg caaatcagcc
    47581 gttcgcgcgt tgcggcgcat attatggatt taatgcgcaa aggccggatt aaaggcaaag
    47641 gttacattct caccgagcag gaatactgcg tagtggtggg gacaatcaat atggatattc
    47701 gcgggatggc ggatatccgt tacccgcaat cggcttctca tcccggtaca attcattgct
    47761 cagcgggcgg cgtgggacgc aacatcgccc acaatctggc gctgttaggc cgtgacgtcc
    47821 atttgctttc agtgattggc gatgactttt atggcgaaat gctcctggaa gaaacgcgcc
    47881 gcgccggcgt gaatgtctcc ggctgcgttc gtttacatgg tcaaagcaca tcgacgtatc
    47941 tggcaattgc caatcgagac gatgaaaccg tgctggcgat taacgatacc catctgctgg
    48001 aacagttatc gccgcaatta ttgaacgggt cgcgcgattt acttcgtcat gcgggcgtgg
    48061 tactggctga ttgcaacctg acagccgagg cgctggaatg ggtctttacc ctcgctggtg
    48121 agatcccggt gtttgtcgat accgtttcag aattcaaagc gggcaaaatc aaacactggc
    48181 tggcgcatat tcacaccctg aaacccactt taccggagct ggaaatttta tggggacagg
    48241 cgatcaccag cgatgctgac cgtaatgctg cagtgaatgc gttgcatcag caaggtgttc
    48301 agcaactgtt tgtttatttg cccgatgagt cagtttattg cagcgaaaag gatggagaac
    48361 aatttttgct gaccgcacca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    48421 ttatggcggg cctggtatat agctttctgg aaggaaacaa tttccgtgac agcgcccgtt
    48481 ttgcgatggc ctgcgcggca atttcacgcg ccagcggcag cttaaacaac cctaccctgt
    48541 ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct
    48601 tgaccgcatc ttatccggct cataaatcgc acactacgct aaaccaataa agaacccggc
    48661 aatggtggca ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta aaccaagctg
    48721 ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaac gcccccacca ccaccccgat
    48781 agaaccaaag ttagcgaaac cgcacaacgc gaaggaaata atcgccacgg ttttagcatc
    48841 cagagtgcca gccgtttgca gatagggtga gaaattgaga taagcgacaa attcatttat
    48901 tgccagtttc tgtccaatca aactcccggc aagattcgca tcactccagt caacacccat
    48961 tacccacgcc agtggggcca gcagataacc tacaatggac tccagcgagg catgttcaaa
    49021 accaaaccag ccgccaacgc cgccgataat accgttaatt aacgcaatga tggcgacgaa
    49081 tgccatcact actgtcgcca cacctgcggc gattttcagc ccggtcattg cccctgtcgc
    49141 ggcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat taaaagaaac
    49201 ctgcgaagat tccgtagccg ggcttagcag gcgggcaaac aagatccccc cggggatcgc
    49261 cattaacgat gccgccagca aatattccac aggcacgccc agtgcggcat aaccaatcat
    49321 tgtcgaacca gcaatcgagg ccatgccgct acaaatcgct gtaaataatt cattgcgatt
    49381 cagccgatcg ataaagggct tcacaatcgc cggaatttcg ttttgcccga ggaaaatggt
    49441 agtgaccgcg acgaatgact caatcttgct gatatttaat gctttctgga atataccgcc
    49501 gagaatgcga attaaaatcc ccatcacacc gatgtagtag agaatactca ccagtgcagt
    49561 gacgaagata attgccggta ataccctgaa accaaagata aatcctgcgc catcaaataa
    49621 ggtgtccatt ttcggtccga ccagagaacc gaagataaat gcgctacccg cgtcgctgta
    49681 cgccatcact ttatgcacac caaaagcgac tttttcagcg acccaacgcc ctggcggtaa
    49741 ccaaagcata atgccgccaa tcacgacctg taacactaac gccgcgccaa cggtacgcag
    49801 gctgatcttc ttcttgttta ctgacagtaa aaacgcaatc gtcagcaata ccaccattcc
    49861 cagaacactt ctcatgacat ccatagtgat tatctcttca tgtcatattg ggcgacggct
    49921 ttctgcccat cagaaatagc caggcctgca tcttgcgata ccggcccggc ttgttatcaa
    49981 cacttaatgg gttttgatgt agccgcgcac gcactcttcg actaagcccc agaaccagtc
    50041 gctatcaata gtgatgccga ctttggtatt agcgggcttg ccaagaacgc ccagctcgtc
    50101 gcagacggta cgcccataac aagggccact gttgacgtcc acttcgacgt acatctcctg
    50161 ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcgt gcaccgggcc
    50221 gccagccagg ccgtagtttt cgaactgggt tttgagagtg aagttcatga tgtcgctgaa
    50281 cagctctccg gcagggccgc ctgccctttc cattcgtgca atcacgtccg gggtgcaaac
    50341 ggtctggttg gtgagatcga ggcccatcat cactaatgga acgccagagg tgaacaccac
    50401 gcgtgcggct tccgggtcag caaagatgtt gaactcggca gatggcgtga agttgcctgt
    50461 accgtaagcg ccgcccatta gcacaatttc acggattttg ggcaggatcg cgggttgcat
    50521 acgcattgcc accgcgatat ttgaaagcgg accaaccggc accagagtga tatcgccatc
    50581 gcttgccatc agggtatcaa tgatgtattt caccgcatga gtgctttctg cctggcgggt
    50641 cagcggctcg aataccgggc catccagtcc ggtatcaccg tgaatattat cggcaacgat
    50701 ttgttgccgc ataatgggtt gtggcatccc cgcataaacc ggaacattaa tctccagttt
    50761 ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac ccgctacaat
    50821 agtgatgcct aataaatcta ttgccggatg tttcgccgcc atcattatag caatagcatc
    50881 atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt atttcctctg
    50941 tttccagttg cgttattttt tctacagcaa agaaatttcg atcgggccga tgattgaatc
    51001 ttaacaacag cgtacgtatg ctaaatatga gaaatctcat agcggataaa catcgtgaaa
    51061 gaaatccaca ataatgatct taagcagcaa ttgatgagtg aatccgcgtt taaggattgc
    51121 tttttaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt
    51181 gtgcaggaag ggcaacagcc gtcctggctg ttttacctga cgcgaggccg cgccaggctt
    51241 tacgccacgc tagctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc cccctgtttt
    51301 attggtgaga ttgagttaat cgataaagac catgaaccgc gtgcggtaca ggctattgaa
    51361 gagtgttggt gccttgcgct ccctatgaaa cattaccgtc cgctgttatt aaacgacacc
    51421 ctatttttac gaaaactttg cgtcacctta agtcataaaa attatcgtaa tattgtttct
    51481 ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcatttat attactctca
    51541 caagaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt aggcgtttct
    51601 tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt aattaaaagc
    51661 aagaaaggat atctaattaa aaacagaaag cagttgtcag ggctggcact ggagatggac
    51721 ccggagaata aattctccgg gatgatgcag taaaaattat ttgcaatagc gcgattgccg
    51781 gatgcaacgc ttgatgcgtt ttatccggtc tacaaattga gcattacgcc agaccaataa
    51841 agaacccggc aatggtcgcg ctcatcaggt tggaaagcgt tgctgctgcc agcgcccgta
    51901 aaccaagctg agcgatttcc ggtgcgcgtt ttggcgaaat agccgaaaat gccccaacga
    51961 caacaccgat agaaccaaag ttagcaaaac cacaaagcgc aaaggagata atcgcaatgg
    52021 ttttcacttc cagcgtgccg cccgtttgca ggtatgggga gaaactcagg taagcgacga
    52081 attcgttaat cgccagtttc tgcccaatca ggctacccgc aaggttggca tcactccagt
    52141 ccacacccat gatccacgcc agcggtgcca gcacatagcc aaaaatactt tccagagaag
    52201 cattggcgaa gccaaaccag ccgccaattc cgccgataat gccgttgatc agcgcaataa
    52261 ttgccacaaa tgccattacc accgtcgcca caccagcggc gattttcagc ccggtcatcg
    52321 caccgctcgc cgctgcttcg ataaagcttt ttggcggcgt ttcgctgaac gacagatttt
    52381 caaacgtgac ctgcgaaagc tcggttgctg gactaagaat acgtgcaaac aaaataccgc
    52441 cggggatcgc catcagcgat gccgccaaca ggtagtcaat tggtacgccc attccggcat
    52501 aaccaatcat catcgaacca gcaatggacg ccatcccgct acaaatagcc gtaaacaact
    52561 cgtttcgatt catgcgatcg ataaacggtt taacgatcgc cgggatctca ttttgcccga
    52621 ggaaaatagt ggtgactgcg acaaaagatt cgattttgct gatgttgagg gctttctgga
    52681 aaatgctgcc aaggatgcga atcagcagcc ccatcacgcc aatgtagtac agcagactaa
    52741 tgagcgcagt gacgaagata atcgccggaa gtacgcgaaa ggcgaagata aaacccgcac
    52801 cgtcaaagag gacatccatt ttcggcccga ccagcgaacc aaaaatgaag gcgctaccag
    52861 catcactgta agacatcact ttatgaacgc ctaatgccgc ctgttctaca gcccactttc
    52921 ccggcggaaa atagagcatg atgccaccaa gagcaatttg cagcagtagt gcggctgcaa
    52981 ccgtacgcaa actgatgctc tttttattca ctgacaacag aaatgctatt gccagtaaca
    53041 ccaccatccc cacaacactt ctcattatgt ccataatgat tttcccttca tgccagtaaa
    53101 cccggcgtca gcgccaggtt tcggtatgtt tgatgagtac gggcgacggc tttctgcccg
    53161 tcagaaatta acccgcgaga cgctgatatt ctttggcaat ttcgctcgcc agaatggcgt
    53221 tgttgaacac aagctggatg ttggatttca ggctgtcacc gccggtcagc tcagcaacgc
    53281 gcgccagcag gaatggcgta ctttctttac caataacacc ctgcgcttca gcttcagcta
    53341 ccgcctgatc gatcaccgca ttgatagtgt gttctggcat cgcaaactgt tccgggatcg
    53401 ggttcgctac cacgaggcca ccgttcagcc cgctttgcca tttcaccgcc attgcacggg
    53461 caatttcact ggcgctgtcg agacgaatgc tgacgtcaaa cgggctggta cggcagaaaa
    53521 acgcaggcag cgctttagtc tgatagccaa ttaacggcac accgaaggtt tctaaatact
    53581 cagtggttaa tcctaaatcg agaatagatt tcgccccggc acaaacaacg gtgacattag
    53641 tatttgccag ttcttgcaaa tcggcagaaa tatcgaaggt atgttccgcc cctcgatgaa
    53701 caccaccaat tccgccggtg gcaaatactt taattccggc aagcgccgca ataatcatcg
    53761 ttgaagccac ggtggttgca ccattttttc ctgcggcaac gacaaaaggt aaatcgcgac
    53821 gactaacttt agtcacgtta tgcccttcac gacccagtaa ttcaatttct tctttgctta
    53881 aacccacttt catcacgccg ccaataatgg cgatagtggc aggtactgcg ccctgtttac
    53941 gaatagtttc ttctacttca attgcggtct gggcattttg tgggaacggc atcccgtgag
    54001 aaataatggt cgattccagc gccacaaccg gttttttgtt ttttaaagcg tcctgcactt
    54061 ccggggaaat ttgtaataat tcaggggaaa tttttaattc agacattctg cgttctccac
    54121 taacgatata acgttggcaa tcgataaatc ggggttattg gtgtattcac aggagagcgc
    54181 cattgacgaa catccctgtg cgaaacgaac agattcggca aacggcattc cgtctaccca
    54241 acacgaagca agtcccgcca tcatggcatc gcccgctccg gtaacattaa taacattggt
    54301 tttgatcggc gcagaccagc cactttcacc gctgatatcg ctgtaataaa cgccgtcgcc
    54361 gcccatgctc aataccagtc ggttcaggcc atgttgatgg aaccaggcag caacttttgc
    54421 cacatcttca cgccctgaca gcgcaatccc actcagggtt tccgcttcaa ggcggtttgg
    54481 cttgagagta tggatttgac ttaatcgctc gcgtactttg acgcatttcc acgcggaaac
    54541 cggatcgaca aatacgggta cgttggcagc attatccaga atccatgcca gtgcctcttc
    54601 actgatatta cagtccgcga caatgacctt tgccctctga ataaattcac ggtgctgtgc
    54661 gagatattca gctgtaatag cgttgctaat attcatgtca tttatagcaa ccagcatttc
    54721 accggtatta tcgagtaatg ataaatagct cgacgtattt tctcccggca caatcaggca
    54781 tttatcgaca taaacgccag attgattggt ttgcgttagc agcgattggc cataaaaatc
    54841 actgcctacg gcgctcagta gccaggcttt attacccagc aacgccaggt tttgtgcaat
    54901 attgcgccct accccaccag gcgtaaattt tattttacct ggatttgaat ccgcataatt
    54961 taatgattca tgtgaatatc cggcgacatc aatattcgcc gagcctataa ttacgacata
    55021 atccttttcg cgcatagcac gtcctctggt aaaaaattac caaccctgtc aatccacaag
    55081 gatatagata ctgcaaagac ggtttaaaca tgtgttcata tttaaacatg tgctcatagt
    55141 atgcatgttt tacgtaaagt aaattgccgc cagagaggca aagcgggcag atttgagcag
    55201 gctcacaaaa tgcgattaaa tgcctgaatt acaaaagcga ggaatttgtg acgggatgca
    55261 cagaacatga acactaaaaa agggcagaaa agaatctgcc cttgaggaaa taaccttagt
    55321 gtttgatgaa cgtttcaggt ttctactgaa agataaaacc atttaaattc ataaaattac
    55381 attcacaaac cggtcattct ctttgcgctc atccaatgat acggggcagt aaatttctgc
    55441 cctgtaacac accttttatt acgcggcttt cgctactgcg tccacttccg gacgtttcag
    55501 gaaggcatag gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccag
    55561 taccggcgta atagcgccag ggatcagcag aacaaacaga ccaccgtgcg gtgccatcag
    55621 tttcgcacca atcgccattg agattgcgcc agtcagcgcc ccaccaacga tacaacacgg
    55681 cagcacacgc atcggatcac gagcagcaaa aggaattgca ccttccgaaa taaagcacag
    55741 accaagaacc agagcggctt tgccaccttc ctgctgcgct ttgtcaaatt tgcgacgcgc
    55801 caccattgtt gccagaccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc
    55861 catcgggcca taggtttgag tactcagcag acccacaccg aatgcgtacg ctgctttatt
    55921 taccggaccg cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc
    55981 attcgcagtc cccatggtct gcagccagtg agtcaaccct tcgagaatgc cagcaaccgg
    56041 tttaccgatc aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat
    56101 caggatcggt ttcagcgcct ccatactctg tggcagtttc agttgcgtac tgattaactt
    56161 cgcaatgtaa ccagccagga agcccgcaat aataccgcca atgaagccag aaccggtgct
    56221 gaccgccagc ataccgccaa tcagacccgg cgtgagaccc ggacgatcgg caatggagaa
    56281 ggcaatataa cctgccagta ccggcaccat cagcgcaaag gctgaaccac caccaatctg
    56341 catcagcgcc gctgccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga
    56401 aagcgcgata cacagaccac ctgcaacgac catcggcagc atgtaagaga cgcccgtcag
    56461 caagtgacgg tatgcgcctg cactctcgtt cttaccttca gaggtcgccg tttgagcttt
    56521 gcccgccggt tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcggtttt
    56581 cttcagcgcc agaccggtag aggtgcgata catcggttta ccagcaaatt tcgccagatc
    56641 cacttcgata tctgccgcca caatcaccag atccgctgcg gctacttctt cgggagtgat
    56701 tgcattaccc gcgccaacag aaccacgggt ttcaactttc acccaccagc cacgtttttt
    56761 cgcttcggtt tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc
    56821 agtcaccgca actacgcgtt tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg
    56881 cgcagtgtaa ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac
    56941 tgcccgggaa atatcgccca gccagacatt tttaccgttc agcgcgctgt cattcgggat
    57001 ggaatcaccg agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga tttccagttt
    57061 tgcttttcgc gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag
    57121 attagcgtca ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt
    57181 gtaagtcgac gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc
    57241 tttgacttac cgccagggct gcaacagcag tcgccagacg cagtgtgtgt tcactggatt
    57301 cacgcattag caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc
    57361 ttacgacatc gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg
    57421 cgccttcggc acccagtgaa ataacgacat gcgcaatacc ttgttcacgc agcgcatgcg
    57481 cagcttcaat cacatctttc atttcaggca gtttacggcc tgcccagatt tccagctcgc
    57541 ggcggttagg tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc
    57601 tactatcaaa gataatgcaa gggcactgac tacgcaggcg agtcatccag tcggtgaacg
    57661 cttccgggct gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga
    57721 gccagctcag agaatcagtc acaaagcgtt cccagtcggc gggggtgact tcaaaacccg
    57781 agaagttgaa gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc
    57841 gcccctgtac aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac
    57901 catcctgatt gtctttaccg aggaagccgc caacggtgac atcaattccc aggtctttta
    57961 atactttggc cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt
    58021 tcacttcgcc gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg
    58081 tgatagtagc aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc
    58141 gccgattgct ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg
    58201 acctttctta acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc
    58261 ggtgccatca aggtttgtca cggtaatatc actgttaaat tgtttaatgg tgttgaccag
    58321 catggtacct ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa
    58381 cacgtcgtcg gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc
    58441 tttcagcaag cggtcagctt tattgtcgag caacaaatca gcgagacgct taagaaccgc
    58501 aatgggctga tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac
    58561 atcaaaagca tttgccgcac ggcttaccgc aattgcgcta cgcagattgc cttcggcgct
    58621 atcgctcagc cagataccct gtccgaggtt cagcggttgt tcattgatgg ctttggtgac
    58681 gaaagtggcg tcaactgccc ccgcctcttt cagacgcgca gcgttcagcg cctgaagagt
    58741 cagcagatcg ctggcgacga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc
    58801 actctgcttt tcgcccatca gcaatgcgcg aagttcttct gctgttgttg ctgacttcag
    58861 ttgttcagca acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccagatg
    58921 ctcatccgag ctggcagcaa taccgatcgc tacgtacgct acctgaccgt caccccaggt
    58981 gacgccttcc gggaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc
    59041 ggtagtgccg tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg
    59101 cgccagcatg ccattgacgt agccttctgc tacattaccg gcctgcacca gcgccgcagc
    59161 gacctggcga atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac
    59221 ggataactgg aacatagttc tcctctcttg ctgaattgaa acgattcagc ttctatgaga
    59281 aaaaaagcgc caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa
    59341 gcataatact tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat
    59401 gtgctgcaca attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa
    59461 tttcatttca gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg
    59521 tggcgctttt gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt
    59581 cctcttccag tgatcgacca gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt
    59641 tgacctgacc tcgacggcgt ttttaatcgt tgcctttctc accggtattg cgggcgctct
    59701 gcaaaccccg acactcagta tttttcttac cgatgaagta catgcccgtc cggcgatggt
    59761 gggattcttc tttaccggca gcgctgtcat tggggttctg gtgagtcagt ttctcgccgg
    59821 gcgctctgat aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt
    59881 gctggcctgc accctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt
    59941 ctttcttagc agctttggct cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca
    60001 tgccgacaaa accggacgtg aggcggtgat gttcagctct tttttacgcg ctcaggtttc
    60061 actggcatgg gtcattggcc caccgctggc ttatgcctta gcgatgggtt tcagctttac
    60121 ggtaatgtat ctgagcgcag cggtagcatt tattgtttgc ggcgtgatgg tgtggctgtt
    60181 tttaccgtcg atgcaaaaag agcttccgct ggcgaccggc acggttgaag cgccgcgccg
    60241 taaccgtcgc gatacgctgc tgctgtttgt catttgtaca ttgatgtggg gctcgaacag
    60301 cctgtacatc atcaacatgc cgctatttat tatcaacgaa ctccatcttc ccgagaaact
    60361 ggccggtgtg atgatgggga ccgccgccgg gctggaaatc ccgaccatgt tgattgccgg
    60421 atatttcgcc aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt
    60481 ctgtttttac gcaggaatgc tgatggcgca ttctcctgtc attctgttgg gcttgcagct
    60541 gctaaatgct atttttattg gcattctggg cggtatcggg atgctctatt ttcaggatct
    60601 gatgcccggt caggcaggtt cagccaccac gctctatacc aacacgtcgc gcgtgggctg
    60661 gatcatcgcg ggatcagtgg cgggcatcgt cgccgagatc tggaattatc acgctgtgtt
    60721 ctggtttgcg atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt
    60781 ttaaggcgcg gtcagcattt ccagatcgat aagccaggtc attgcctgct gacgcgagtt
    60841 accgcacatt tcagcgctgg cttgtaagcc tgcacagact ttcggccgca gtggcgaggt
    60901 gaaaatttta cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc
    60961 atctggcata ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc
    61021 cggacggcat tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca
    61081 gtaccacctt ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gctcgtcgcg
    61141 agtactttgt cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc
    61201 gaacgaaatt aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga
    61261 cattgatatt cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc
    61321 tgatgtccgt accgggctga aagtagaaga gcgtttcaaa ggagatgata tcgttgacac
    61381 cgtgacgctg acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt
    61441 tatggataaa gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt
    61501 gctgtttatg ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact
    61561 gctggcgctt gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat
    61621 caaaggggca tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat
    61681 tcaggtacca gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg
    61741 ttatatgggg cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc
    61801 ccttttttaa tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc
    61861 aattttcact cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt
    61921 tcaattgctg tcatataact ttacactgtc attgttaatt aatcgttact aagacatgac
    61981 tcctatgaat acaatcgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga
    62041 tcgccagcag ttgcaatcac gtatcgttca ttttggcttt ggagcctttc accgcgcaca
    62101 tcaggcgtta ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga
    62161 aatcagcttg ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata
    62221 taccgtgctg gagaaagggg cggacggcaa tcaggcgata attgtcggtg ccgttcacga
    62281 atgcctgaat gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca
    62341 ggtggcaatt gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg
    62401 tgcactcgac accagcaatc cgcggattat tcacgatcta caaacccctg aagaacctca
    62461 ctccgcaccg ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc
    62521 gtttaccgtg ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt
    62581 gctgggaatg gcagaaaaac gttcgccaga actcgccggg tggataaaag aacacgtcag
    62641 ttttccggga accatggtcg accgcattgt tccggctgca accgacgaat cactggcaga
    62701 aatcagccag catctggggg taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca
    62761 gtgggtggtg gaagataact tcgtcgctgg gcgtcctgcc tgggaagtcg caggtgtaca
    62821 aatggtgaat gatgtcctgc catgggaaga gatgaaactg cggatgctta atggcagcca
    62881 ctcttttctc gcttatctgg gttacctctc aggattcgcc catatcagtg attgcatgca
    62941 ggatcgcgca tttcgccatg ccgccagaac attaatgctg gatgagcaag cgccgacact
    63001 gcggattaaa gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa
    63061 tccggcgctg aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca
    63121 acgcatgctg gcaggtattc gcatacatct ggggcgcgaa acggactggt cgttgctggc
    63181 attaggcgtt gcaggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat
    63241 tgatgttcgc gatccgctta gcgataaaat tcgcgatctt gttgcgggca gcatcagtga
    63301 gcaacgcgtc accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa
    63361 cccgcatttt gtgcaggcca tcgaacaagc ccggcaacaa atcgcacaat tcggcgcgca
    63421 tcaggcgcta ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct
    63481 cgccttcgtc cgctctcccc ttctcttttc tgccgttttt agccaggatt aacgctcagt
    63541 taacttacca gagtattgcg gagctgttgt gaccaggacc aacctcatca ccggttttct
    63601 cggcagcggg aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa
    63661 atgggcggta ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga
    63721 tagcggcgca ctgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc
    63781 catgcaggta gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga
    63841 gccgactggg ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga
    63901 accgtggata gatctgcgcg ccacgttgtg cattctcgat ccacgcctgc tgctggacga
    63961 aaaaagcgcc agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc
    64021 caataaatcc gaccgtgcga cacctgaaag cgagcaagcg ctacagcgct ggtggcagca
    64081 aaatggtggc gatcggcaat taattcacag tgagcatggg aaagttgccg gtcatcttct
    64141 ggatttgccg cgtcacaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca
    64201 tgtcgtgaaa aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag
    64261 tctgaacagt ggacaaggac atcaggcctg cggctggata ttcgacgctg atacggtatt
    64321 cgacaccatt ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt
    64381 gctgcgtatt cccgaagggc tggtgcgaat caaccgtcag ggcgatgacc tgcacattga
    64441 aacgcaaaac gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga
    64501 ctggaatgcc ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg
    64561 cctgcgtttt tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg
    64621 ttgttgaata ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga
    64681 ttctggttgc cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt
    64741 aaggcctttt tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttcactg
    64801 ctggcaatgg gaatgttgat gctgagtttc tggctgaaag aaaacgcccc aggcagacga
    64861 cgtatcgtga ttattggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt
    64921 caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc
    64981 gtcagcgaac tgctctctgt ccccacgaaa gatgcctcac gagatagctt tcccggcgat
    65041 cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca
    65101 ggtcttatcg cccttattat ttttgtggtt tttgcgtttc ccagagtaat gattggcgca
    65161 cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg
    65221 gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga
    65281 aaaaacaaac atttccaaaa caaataactc acagtactta acatcatcag gtttattttt
    65341 atagtgagga taatcctgat gatgcgcacc gtgctttcat ccatcgaaag caaaaatcat
    65401 tctctaagta aatgaatgga ttgcatgcat ttcactcaat cgtactttaa ttgaccaatc
    65461 ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca
    65521 agaccgcacc aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca
    65581 gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc
    65641 tggtaattag tctcgtgtcg cttggcattt tttttataac gatatttgtc gttaaggact
    65701 tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg
    65761 gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa
    65821 gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca
    65881 ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta
    65941 tgctgactgg aaaggcgtac gttatcgtct gggcggcagc actaaaaaag gtatcgattg
    66001 ttctggtttc gtacagcgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac
    66061 ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt
    66121 agttctgttc cgtgccggtt caacgggacg ccatgtcggt atttatatcg gcaacaatca
    66181 gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccgtactg
    66241 gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat
    66301 gcaatccctt ggctatcctg acgagttaac tgaaagcact gtttaggcag tgcttttttg
    66361 ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat
    66421 tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc
    66481 tcccaattct ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtaa tgattgcgat
    66541 actggtgagc tgccttcagt ttttagtggc ctggcataag cacgaagtca aatacgacac
    66601 actgattacc gacgtacaaa agtatctcga tacctatttt gccgacctga aatccactac
    66661 tgaccggctc cagccgctga ccttagatac atgcaagcaa gctaaccccg aactgaccgc
    66721 ccgcgcggcg tttagcatga atgtccgaac gtttgtgctg gtgaaagata aaaaaacatt
    66781 ctgttcatct gcgaccggtg agatggacat tccactcaat gaattgattc ctgcgctcga
    66841 cattaataaa aatgtcgata tggcgatctt acccggcacg ccgatggtgc cgaacaaacc
    66901 cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggcgtct ttgccgctct
    66961 gaatctcaac ctgacgcctt ctctctttta tagctcacgg caggaagatt acgatggcgt
    67021 cgccctcatt attggcaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa
    67081 cgaattaacc gacatgccag tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg
    67141 gctttatgca gatgactgga catggaacga tgtgtggtac gcatttttac tgggcggcat
    67201 gagtggaact gtcgttggcc tgctctgcta ttacctgatg agcgtacgta tgcgccccgg
    67261 cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggcgtatc aaccggtggt
    67321 ggatacacaa gctttgcgag taacgggcct ggaagtactg ctacgctggc ggcatcctgt
    67381 cgcgggagaa attcccccgg atgccttcat taactttgcc gaatcgcaaa agatgattgt
    67441 gccgctgact cagcacctgt ttgagttaat tgcccgcgat gccgcagaat tagaaaaagt
    67501 gctgccggta ggcgtcaaat ttggcattaa cattgcgccg gaccatctgc acagcgaaag
    67561 ctttaaagca aatatccaga aactgctcac ttccctaccc gcacaccatt tccagattgt
    67621 gctggaaatt accgagcgcg atatgctgaa agagcaagaa gccacacaac tcttcgcctg
    67681 gctgcactcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc acagcgcgct
    67741 tatctatctt gagcgtttta cgctcgatta tctgaaaatt gaccgtggat ttatcaacgc
    67801 catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg
    67861 cctcaatatg ctgacggttg ctgagggggt cgaaacgccg gaacaggcgc gatggctaag
    67921 cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgcccgttac cgctggacga
    67981 ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct
    68041 tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccgtt
    68101 gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgcgcaggc
    68161 gcaggctatc aaggaaagct atgcctttgc cgtgctgggc gaaccccggt acgcgtttaa
    68221 tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga taacgttgtc
    68281 agccctcggt accttcgata atttcaaccg ctatgcactg cgcggcaacc cgggcgcacg
    68341 caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta
    68401 cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat
    68461 taatcctcgc gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac
    68521 ttttcaaaaa tttatgactg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac
    68581 cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta gctaaacccg gcaaagaaga
    68641 tatgttgagt ctgttttcgc tgccggtatt tccagaaaag tactggaagg atcacaaact
    68701 tagcgatccg ctcgccacgc ctccgcttgc cagtggtccg taccgaatta cgtcctggaa
    68761 aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt
    68821 aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt
    68881 cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg acgccaaaaa
    68941 ctgggccaca cgctatagcg gtaaaaattt cgataaaaaa tacatcatca aagatgagca
    69001 aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccggtatt
    69061 cagcgatcgc cgggtccggg aagcaatcac cctcgccttt gactttgaat ggatgaacaa
    69121 ggcgttgttt tacaatgcct ggagtcgcac gaacagttat tttcagaata ccgaatacgc
    69181 ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga aaaaagattt
    69241 accgccagaa gtcttcacac aaatctacca gccgccggta tcgaaaggcg atggctacga
    69301 tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa gcgggctggg tgctgaaagg
    69361 tcagcaacgc gttaatgcca ctacgggtca gccgctcagc tttgaattat tgcttcctgc
    69421 aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa
    69481 catggacatt cgcaaggtgg atcactcgca aatcaccaac cgcatgcgca gtcgcgacta
    69541 tgacatgatg ccgcgcctat ggcgggcgat gccgtggccc agttccgatt tacagatttc
    69601 ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa gcccggttat
    69661 cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt
    69721 aggacgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat
    69781 ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtgc gccctgttta
    69841 cagtctgggt atcgatacct ggtggtatga tgttaataaa gcggccaaac tgccgtcagc
    69901 cagacaacag ggagagtaga tgggcgctta tctgattcgc cgtctgttgc tggtgatccc
    69961 aacgttatgg gcgattatca ctatcaactt tttcatcgtg caaattgcgc ctggcggtcc
    70021 ggtcgaccag gccatcgccg ccattgagtt tggtaatgcc ggagtattac ccggcgcagg
    70081 cggtgaaggc gttcgcgcca gccatgcgca aacgggcgtc ggtaatatca gcgacagtaa
    70141 ttaccgtggc ggacgcggat tagatccaga agtgatcgcc gagatcactc atcgctacgg
    70201 tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca tccgctttga
    70261 ttttggcgat agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc
    70321 ggtttccatc accctcggat tgtggagcac gctgattatc tatctggtgt cgattccgtt
    70381 aggcattcgc aaagctgttt ataatgggag ccgctttgac gtctggagta gcgcatttat
    70441 catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc
    70501 aggcggcagc tatttcgacc tgttccctct acgcggcctg gtttccgcta actttgattc
    70561 gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctgc cggtgctggc
    70621 gacggtgatt ggtggctttg cggcgctgac tatgctgaca aaaaactcat tccttgatga
    70681 agtacgcaag caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaaa aaaatattct
    70741 ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt
    70801 tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac tcaatggtct
    70861 tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt
    70921 gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct
    70981 ggtcgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcaaccc cgtcaatcag
    71041 gcccgttggg cgcgttttcg ccataaccgt cgcggctact ggtcgttatg gattttcctc
    71101 gttttgtttg gtttgagttt gtgttctgaa cttatcgcca acgataaacc gttgctggtg
    71161 cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag cgattttggc
    71221 ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg gctggaaaat
    71281 aatggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc
    71341 gataagccct tcccttctcc cccctcccgg caaaactggc tgggaacgga tgccaacggc
    71401 ggcgatgtgc tggcacgtat tctctatggc acgcggatct cggttctgtt tggcctgatg
    71461 ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg ctattacggc
    71521 ggtaaggtcg atctctgggg gcagcgcttt attgaagtat ggtcggggat gccaacgctg
    71581 tttttgatta ttctgctttc cagcgttgtg caacctaact tctggtggct gctggcaatt
    71641 actgtcttat ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtacc
    71701 cgcaatttcg actacatccg cgcggcacag gcgcttggcg tcagcgatcg cagtattatt
    71761 ctgcgtcata tgttgccgaa tgcaatggtc gctaccctca cctttttacc gtttatttta
    71821 tgtagttcga tcaccaccct gacctcactc gatttcctcg gcttcggtct gccgctcggt
    71881 tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc tccgtggctt
    71941 gggatcaccg ccttcttgtc ggtggcgata ttattgtctt tgctgatctt tattggtgaa
    72001 gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc
    72061 gattgaagat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag tagtcaatga
    72121 tgtttcacta cagattgagg ctggcgaaac gctggcgctg gtgggtgagt caggttcagg
    72181 caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg ttgaatatct
    72241 ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgacc aaacgttgcg
    72301 cggtgtacgc ggtaataaga tcgccatgat ttttcaagag ccgatggtgt cgttaaatcc
    72361 attgcatacc ctggaaaaac agctttatga agtgctttca ctccaccgcg ggatgcgtcg
    72421 ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gttggtatcc gccaggcggc
    72481 aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat
    72541 tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccga ccaccgcact
    72601 ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagttgaa
    72661 tatgggcatg ctgtttatta ctcataacct cagcattgtc agaaaactgg cccaccgcgt
    72721 ggcggtaatg caaaacggtc gctgtgtcga gcaaaattac gccgctacgc tatttgcctc
    72781 acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg atccagtgcc
    72841 gttgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg ccttccccat
    72901 tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt
    72961 tacgctacga gcgggtgaaa cactgggttt agtgggcgag tccggttccg ggaaaagcac
    73021 gacgggactg gcgctgctgc gactgattaa ttctcagggc agcatcatct ttgacggtca
    73081 gccactgcaa aatttaaatc gccgccagct gttacctatt cgtcatcgca ttcaggtggt
    73141 atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga ttattgagga
    73201 aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac aagtgataga
    73261 cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc
    73321 tggtggtcag cgacaacgta ttgcgattgc cagggcatta attcttaagc cctcgctgat
    73381 catacttgat gaaccgacat catcactcga caaaacggta caggcgcaaa tattgacgct
    73441 attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca
    73501 cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca
    73561 aggaccgtgc gcgcgcgtgt ttgccacacc gcagcaggag tatacgcgtc agctactggc
    73621 gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa
    73681 aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg atttacaaac aggcacggct
    73741 caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcctaacgcc tggcttagtt
    73801 tatacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc
    73861 tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag
    73921 cagaccagcg atagttctgc ggcagtcgat ggacgattga gagttgtaat gaagtcactt
    73981 attttccccg gaagcacatt tacttcacaa tttgtttaca tcaattttaa cacatcatca
    74041 acaatccgtc tttatacaga tcgtaaagat tgatgcctcg cctgcgtggc ctctatggct
    74101 ctgatttaag taatatctgc gggcgtggtc acgtttgcgg ggctatatgt acccgtttct
    74161 gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa ttgattttac
    74221 ataaaataaa catatatcgg gggaatgata gatttgtggt tgacatgcat caacaaagga
    74281 agccttttag cttcctcgtt gtgcaataga tcaccgtttt ttcggccgac tggcgtacag
    74341 acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg gccatgcaga
    74401 gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa aacggaaggt
    74461 tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa tgaccgccat
    74521 cgcattggat gacaccatcg acacgcagcc cacaaacgcc gcaacgccaa ccaccagcga
    74581 ccaaaatccc agacccagca gcgcactgat gaccatccac gctgccataa taaattgtat
    74641 ccacaacccc gagcggaaca tatttaacgc gccaatacgg cggacgaagc ggctattaaa
    74701 gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa agttttccgg
    74761 cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg cttaagaacg agaacatccc
    74821 ggcaaagctg aaaccactgg ccagcatata actcaggaca cgcttatgac ggaacagcgc
    74881 cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg cgacgctccg gtggtaaggt
    74941 ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg cgaggatcca
    75001 gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag
    75061 cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg
    75121 gtagatatca cgcatcaaag cgttgataac cacgctggcc gcagccgccg ccagcccgtg
    75181 gaagaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc
    75241 ggcggcaaac accagcgtac cgccaagaac caccggctta cgcccgaagc tgtctgccat
    75301 cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga gggtcatctg
    75361 cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat
    75421 atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga
    75481 cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct
    75541 ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt
    75601 aatgccgcca atacgttcac gatgcagctc aaccacatgg ttacccacgg cggcgaacat
    75661 gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat
    75721 cacttcaagc accgctggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc
    75781 aaattgctct gccgtatcgt cagctacggg tgattccagc gtcaccagat aggtcttctc
    75841 gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatctg tcattagtac
    75901 cagaccggtg gtatcgatat ccagccgccc tgcggcgtgc agtttccacg ccactggttc
    75961 atctagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa catagccctg
    76021 aggcttattg agcatgaagt aacgtggacc gtgttgctgc gccagcgggt tgccatcgta
    76081 agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cggacgattt cgccatcgac
    76141 ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg
    76201 tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg aggtcgggca
    76261 ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac ttccctcaag
    76321 ggaaaaagaa ccgtggcata ctattagcag aataatctac ctacgcgaga ccatgatttt
    76381 tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca
    76441 taaaacccct gccgttatcg tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga
    76501 acttgcgcgg ctggctcgtg gtcgcgtgct ggtgctggca cacgttaaag aactggtggc
    76561 gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgccgggct
    76621 aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg tcgcccgtaa
    76681 tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgtc accgtattgg
    76741 tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgacaaaag tgaatcccca
    76801 cttacgcctg ctggggctga ctgccacgcc ttttcgactg ggcaaaggct ggatctacca
    76861 gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta
    76921 tgaactgccg ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga
    76981 tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctattcag
    77041 cgaagccgat ctcaaccgcg agctaaaaaa acaacaacgt attaccccgc acatcatcag
    77101 ccagattatg gagtttgctg caacgcgcaa aggggtgatg attttcgccg ccaccgttga
    77161 acacgcaaaa gagattgtgg gattactacc cgccgaagat gctgcactga ttactggcga
    77221 cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta
    77281 tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc
    77341 cattctgcgc cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg
    77401 tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc ctcacgatct
    77461 ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt
    77521 ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt
    77581 gattgaacac tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga
    77641 acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat
    77701 tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa
    77761 agcggcgcta cgactgaaag atgcgctggt attacgctgt agcggcatgt ctttgcagca
    77821 tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga
    77881 tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg agcagctttt
    77941 tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat
    78001 cctcgcccag caagccttat tgcgacaccc ggattttgtc gttgcccgca tgaaaggtca
    78061 gtactggcaa gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga
    78121 attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc
    78181 cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta
    78241 atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg
    78301 gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag
    78361 aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacattcaa gctaaagctg
    78421 aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc
    78481 aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt
    78541 aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta
    78601 tgcctgatta cgatattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg
    78661 cattcataac acaaaaccga ttaattgccg ccggacgtcc ggcgctgcaa ttgatcgcgc
    78721 aaattcggcg gcgtgccttt aatagtcagg gtatcggtcg ccggatccca gaaaatccgt
    78781 tcgcccaaca gcatcgcatc aaaattaata gtcaagccac cgccgctacc ggcaaacttc
    78841 gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt
    78901 tcagccgcaa actccgtgaa gctcacttcg ctgacgcctg ccagctcttt cgacagcgat
    78961 tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg
    79021 cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct
    79081 tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc
    79141 ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtc
    79201 gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg
    79261 atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat actgctcagg
    79321 ttgctcagga ctgccaccag cagatactcc accgccagat aacggtagtg gcaaaacagc
    79381 acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accggttgcc
    79441 gcgcggctaa atgccagaaa gtcctcttca ccctgacgct gtaagcgcag cgtctgcgcc
    79501 agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc
    79561 agttctgcta ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc
    79621 agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc
    79681 agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga
    79741 cgcaataaaa gatggcggtc attttacaga aggcggcgta acgagaatga tttaacgcat
    79801 aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga
    79861 tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca
    79921 gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta
    79981 ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt
    80041 gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg
    80101 aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat
    80161 ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag
    80221 ccgttacctg tttatcgccg actggccgac cacgcttgct ggtcgcattt attcctacgt
    80281 aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga ttctcttccc
    80341 gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac
    80401 ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct
    80461 taatcccatc gtctggcaac tggttattaa cccagacgaa aatgagatgg cgcgcgactg
    80521 gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg
    80581 gagctggcaa aagctgcgca gcctgacgcg tcgtcggcgc ttcgcgcgtc cgctggccgc
    80641 attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt
    80701 ctatcgccct atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg
    80761 acgttttctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc gtcttattga
    80821 gcaaggtaat ccagacgccg tttccgttca gtatccgtta agcgaactgc gctatcgcga
    80881 tatgggcacc gggcagaatg tcttgttgat tactgtcgat ggcctgaact actcacgctt
    80941 cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca
    81001 tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc
    81061 gagctatatg gacggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa
    81121 tcagcaaggc tatcagctgg ggttattctc atcagatggc tttaccagcc cgctgtatcg
    81181 ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc acccaatccg acgagcagac
    81241 cgccacgcag tggatcaact ggctgggacg ctacgcacaa gaagataacc gctggttctc
    81301 gtgggtctct ttcaatggta ctaacattga cgacagcaat cagcaggcat ttgcacggaa
    81361 atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgcg
    81421 tgattctggc aaactggaca atacggtggt gattatcact gccggtcggg gtattccact
    81481 gagcgaagag gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat
    81541 tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat
    81601 gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg
    81661 tcaggatttg ttcaaccctc aacgccgtca ttactgggtt accgcagcgg ataacgatac
    81721 gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac
    81781 ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca
    81841 agtactgaca gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa
    81901 atatcttact tgcaatcggt gtggaaaacg gtagtattaa caaccacgag tcggcacgta
    81961 gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt
    82021 gccgaccaaa aatcccaaaa aaaccaaccg taaaggttgg tttttttata tctgcattta
    82081 attcgataaa cagaccgcga cacatcaggg cctgtttatt gtctgttatc agaacgtcca
    82141 gaccacaccc gcctgggtat tccacggcga ttctacccct gcgccattgc catagctgac
    82201 tgacaaatga ccgcttaacg tcggggtaaa cgatgacctt atcccagcct gataaacgcc
    82261 acgggttcca gccacatcat tattaaattt gccatcgtca ttaactttca cctggttaga
    82321 atcggcgtat tcctgacgca cggcggcttt cagccagggt tccagcgtcg taccgttttg
    82381 caggtccatg tgatagctta ccgccgttcc cgcttcagcg cgtaatatcc gggtatttcc
    82441 cacatccgcg cgcatgccgt ttgataacgt gtagtcctga ccatctgtgg taaagccggt
    82501 aaaggccaga tagggtctaa cactccacaa tccgtcaacc caacggaacc cgctctcgac
    82561 atgagcaccc gcgccgttac tattgtaatc gccaaacgct gttgccccat tactcatctt
    82621 gccatggatg gtgttggcaa aacggtcaac tttcaccact ccatcaacat aggcaccgtt
    82681 ctgatgctcc caaccggcat aagcccccag ggtatagcta tcgacattgc ctttgccgcc
    82741 gcgatcaaaa ccaatatcag aatgagagta accaaagaac aagccgcgaa ttgtgctgct
    82801 ttcttcacgg gagaaacggc tatcgatacc gagcgtcagg cccgtcaatg tttgctcaaa
    82861 accagctccc gcatcagtgg tcacgttgtt gcgggtgtta attgccgaac tccacatcgc
    82921 cgtatcgtaa ctaacgcctt ttacgctacc aagacgctca cgcacggtgt ccagttctgc
    82981 atcaaatacc agcggttgtg cggccgccat attcagcaca tcagtggttg aaggggtaat
    83041 ttgcgcgcga ttctctgcca gactccagct atggttacca ttatccagca aggtatattc
    83101 atacgtaccg atatcaacaa cgcctccggc attgcccaac gtaaatgcag catcaccgcc
    83161 gcccgttgtt accagtgtaa ggctatctcc tgctgccggg ctggcaccgg tgtccgtcac
    83221 gaatattttg aaatcacctg ttgcctgacc ggtgacgttg agctgatcgc tctgatgatt
    83281 cgccatatcg gtacgcatat aaaaattgcc gtttcccgac agggtattgg tggtcaacgt
    83341 agtgaaattt tccgcacttg ccgtcacaga tgaacgagtc actgactcat ccatcataat
    83401 cacatttgcg ccagacatcg caaggttggc taagttaagg cgggtgtttt ggttcgtcgt
    83461 agtgatatcg gcaatgatgg tccatgcgct attttcgagc gaaacatccg ctttgctggt
    83521 atccacggca ccatgcgttc ttaaagaagc gccctcagaa accactacct tacccaccaa
    83581 cattgcgggt gcataattta tttgcggctt cataacctca agaatgccat catttcctct
    83641 gactgacacg ttaaccattg tgccagccca gacgttagcg cggccattgt tgataaccat
    83701 attttcagag atggctttag aggaatacgt tatacttcca ttctgatctg atcgaccgag
    83761 atcatatacc gcatttgcct gcactctcgt accagtatcc tttcccagcg attgcatagt
    83821 ggcatgctca tcgaggatag tgtcaatggc ctgcgtgtcc tccataacaa taagcccgga
    83881 accatcatcc agttcataat tttttgacac accatctttt atactaaatt ggcctttact
    83941 gtttgtacca ctcacttcca gcgcattcgt tgagacaatt aattttccgc ctgctttttt
    84001 atcaacgcca gttgcagtcc cgccatccat aacctccagt aagccgccac tatctacagt
    84061 agtattataa gcgaagtcat tttcttcaac tcgcaaactt ccgccgtttt ccaacagcat
    84121 attgttagca ataccattct tgatttcgaa ctgaccgaga cggtttgttc cgaatacctc
    84181 catgacccgc gtgcttgctt taatagcacc gcctgctttc tgatctaccg caatcgctaa
    84241 tcccccctct ttaacctcca gtaatcctcc cttttcgata gcggcaagga cggtaatccc
    84301 atctttttca acgattaagc gcccgccttt aatcgtggga tcattcgctg cgccataaac
    84361 ctgtagcaca ccattattga gcgtagtctg gtttgccgcg ccacccattt gaacaatttg
    84421 ataaccatca tttaatactg agtattctgc ggtgccattc atttcaatgt actgataccc
    84481 gccgttaatt tcagtattac tacttatacc aaattcttta atatgctgtt ctccgccagg
    84541 attgattttc gtattattgg cggtcccata aaccagctgg atatcttttt ccaggacaac
    84601 accatcaact gtctcaccat actcaactgt tgatgccaat gctatgggtg aatataacgc
    84661 tgtagctacc agaacagata ttgcggaacg acaaagagaa acagaaccag attgatgcat
    84721 tgagctttca tcctatgaaa ttaattgcag ttaaaagcat tgggtacaga aaatacccat
    84781 agctccatac ccggagtcag tttttaaaaa ctgtttaaag aaatgcacaa gtattgtgat
    84841 tgatttttta gttgtttttc ttgatgagaa gctgatgcaa aattccgtct ttataatgaa
    84901 aatgatgcca aagcgaacga caaggttgta gttttcgcta catgtccata cataaaatgg
    84961 ggtaacattc acgcgcctgg tagcgttacc aacgctacgc tcaaacataa tgattctaat
    85021 aaaacctcag gagactacta tgcctgaagc aacgcctttt caggtgatga ttgtggatga
    85081 tcatccactt atgcgacgcg gtgttcgtca gttactggag cttgatcctg gctttgaagt
    85141 ggtcgccgaa gcgggcgacg gcgcgagtgc tatcgatctg gcgaatagac tggatatcga
    85201 cgtgatcttg ctggatctca atatgaaagg tatgagtggc ctggataccc tcaatgcctt
    85261 gcgcagggat ggcgttaccg cgcaaattat tatcctgacc gtatcagatg cctccagcga
    85321 tgtctttgca ctgatagacg caggcgcaga cggttatctg ttgaaagaca gcgacccgga
    85381 agtgttgctg gaagcgattc gtgccggagc gaaaggcagc aaagtcttta gcgaacgcgt
    85441 caatcagtac ttacatgaac gtgaaatgtt tggcgcggaa gaagatccct tcagcgtgct
    85501 gacggagcgc gagctggatg ttctgcacga gctggcacag gggctgtcaa ataaacagat
    85561 tgcctcggtg ttgaatattt ccgagcagac agtaaaagta catattcgca atctactgcg
    85621 caaactcaat gtccgctcac gcgtggcagc caccattctg ttcctgcaac aacgcggggc
    85681 acaataaaaa tagcccgatg gcatcaccat cgggcctctt tttatttact ctcctgcggc
    85741 gacaaatgtt gcatcgcctg cgcgatacta cgttcaatca ccgcacggcg agtatcgttg
    85801 gcaggtaaga gtttcaacat catctcccac gcggcaacgg cttcgccaaa tcgctgctgc
    85861 tcaaaggcat taaacgcata catgcttaac acacgaatat tgctatggtc cgttctcacc
    85921 aactggcgca gcagttcgcc gccgaggcgg ttgtcgttgg gatcagatga acgtgtcaac
    85981 gcttcagcat aacccagtgt ggcatcgctg tttttcggat cgaggcgata cgcagtagcg
    86041 tatgcatcgg tggcgatact ggcgttaccc agcgccatgc caacgcgacc caacataatc
    86101 caaccttcta tatctcccgg atttttttgc agttgagtac gcatccccag cgcaaggcgc
    86161 gacatctctt cttcgttgag cggatcggct ttcggatcca gcgccctgtc cagcaacgcc
    86221 ggagcctgtg ccgtggcctg ctgccagatt ttcacctgct gataattgcc agtctggtag
    86281 tagctgacgc cagccacaat taacgccacc acaatacccg gcagataaac aacatatccg
    86341 gcacgcttac cttccggcac gctttgttca ggaaacgctt ccggtaccac gcgtacccga
    86401 cgccgcgaac gggcgtatat gacccaaccg ccaataccaa tagccactac cggaagcacc
    86461 cacagcagca cggtcagcgg cgttaacggc ggatcgtaag tgacgaagtt gccgtaacgc
    86521 gccaccatat aatcgacaat ctctttctta cttttgcctt cctgcatcag ttcatacact
    86581 ttctggcgca ggtcggtggc aatcatcgag ttggaatcgg caatgctgtt attctggcat
    86641 ttcgggcagc gcagttcttc agtgagctga cggaactgtt gttcctgtgc ttcatcttta
    86701 aactgcaaca cgtcgatggt cgccagcgct gagccggaga tcatcagcat cagcacgccc
    86761 aataaaaacc tcattgtgcg gcctccttac tgtatttctc ccacagcggc ttgatctctt
    86821 cttcccagac gcgaggattg agatcgcccg catggcgata gcgaatgatg ccgttgccgt
    86881 caataagaaa cgtttctggc gcgccataga caccgagatc cagccctaac atgccatcgc
    86941 catcaaaaag gcttagcgcg taaggattgc ccagctcttt cagccagctg attgccttct
    87001 ggcgatcgtc tttatagttc atgccgacca cgcggatgcc ctgcgcagaa agctgattca
    87061 gatattgatg ttccgcacgg caggtcggac accaggtcgc ccagacgtta agcagcactg
    87121 gtttgccctg agtcagcaca tccgcctgat aaaactgccc cggattgtcc agtgattcga
    87181 gacgaaactt cggcacaggc ttgccaatga gcgccgattc cagattggtc ggatcatccc
    87241 cttcggcatt acgcgccagc tgccacagca gcgccgcggc aatcgccagg aagataatca
    87301 acggaattaa caatactttg cgcttcatac agcctccggc gcagtttttt gcgggctcac
    87361 gcgcttacga tagcgaggat caaacagaca cagcagtccg cccaacgcca tcatcagccc
    87421 gcccgcccaa atccagcgaa caaatggttt gtagtaaaga cgcacagccc acgcgccgtt
    87481 ttccagctct tcaccgagcg cggcgtacag gtcacgcgtg atgccgccgt caattgccgc
    87541 ttcggtcatc atcgacccgg cagtgttgta ataacgtttt tccgcataca gcaccgtttc
    87601 cggtttaccc tctcgcgtta cgccgatagt cgccacaccg ccacgccagt tcgggccagt
    87661 cacctctttg acatcacgga aggtgaagcg atattcatga atatcgacgc tatcgccgga
    87721 cttcatgcgc acatcacgct caacgctata gttctggcta aaggcaatgc caacaattgt
    87781 cactgccagc cccaggtgag ccgccaccat cccccaataa ctgaaggtgg ttttcgtgcc
    87841 gcgtgaaata cgtagcgcag cttccgcaat tgccagcacc gcaatccagc aggccattgc
    87901 caggccgagc accgtcatcg ccacaacttt gctttcgaac agccacggca acagcagaga
    87961 cagcaccagc gtagagatga aggcgataat caataaattg cggattttac gtgggcgatc
    88021 gcgcccccag cgcaccagag gaccgacacc cagcagtagc gcaaacggca ccatcagcca
    88081 ggtaaacatg gtgttgaaga acggttcgcc aatcgaaata ctgcccagtc ccagttgctt
    88141 gtgcaccagc ggcagcagcg tccccagcaa caccaccagc atcgcggcga ccagcaaaac
    88201 gttgttcgct aacagcaaag attcccgcga ccacagcgca ttgtttacgc gtgagcgaac
    88261 tttgtgtcca cgcgcggcaa acagcagcag cgaaccgcca atcaccagca ccataaaggc
    88321 gaggataaac ataccgcgcg ccggatcaga cgcaaacgcg tgtaccgata ccagcacgcc
    88381 ggaacgcacg aggaaagtcc ccaacagaca caacgagaag gcactgattg ccagcagtaa
    88441 tgtccacgct ttgaagctgg cgcgttgttc agtgaccgcc agtgagtgca tcagcgcagt
    88501 ccccaccagc cacggcataa acgaggcgtt ttctaccgga tcccagaacc accagccacc
    88561 ccagccgagt tcgtaatagg cccatgcgga accgagcacg atgccgagcg tcaggaagat
    88621 ccacgctgcc agcgtccacg gacgagtaaa acgcgcataa gtgctgtcca gacgcccgct
    88681 cagcaaagaa gcaatggcaa aagcaaacgc caccgagaaa cccacgtacc ccatataaag
    88741 cagaggcgga tggaagatca gccccggatc ctgtaacagc gggttaagat cgcgcccttc
    88801 aatcgggaag ttcggcagcg tgcgtgagaa tgggttagag gtaaagagaa tgaacagcaa
    88861 aaagccgaca ctgaccatcc ccattatcgc cagtacgcgg gccacaatat ccagcggaat
    88921 acgctgacta aaaatcgcca ccgcaaaggt ccagccgctc atcagcagca cccacagcag
    88981 cagtgagcct tcatgtgcgc cccaggtagc cgccacgcga taccacaccg gaagctgggt
    89041 attggagttg ctggcaacat aggtgacggt aaagtcgttg accacgaaag cattgaccag
    89101 caccagaaat gcgccagcca cagacataaa cagcagccag gcaaacaagc gggaagacgc
    89161 catcatgcgc gcatctccgc gcgccacgcc ccatagcgga tacacggaca gcagcagcgc
    89221 aattcccagc gccaggcaca gcagcccgtt accaatttcc ggcatcatga tgctgggtcc
    89281 ttataaacac tcgccgggcg acggtggtta gcttccatcg ctttctcaac ttctggcggc
    89341 gtgtagtttt catcatgttt cgccagcact tcttttgcga ggatatgatt gcctttttcc
    89401 agctcgccct gcaccacaac gccctgccct tcacggaaca gatccggcaa aatgccttcg
    89461 taagagacat ccactgagcc ttcagcgtcg taaatggtga aggtcacttt cagcgaattg
    89521 ggatcgcgct gcacactacc cggcatcacc atcccgccaa cgcgcagacg ctgaccgact
    89581 tccggcatct gctgagtttc acgcttgccg tagagaattt cccccggcgt ataaaagaga
    89641 tcgatattcg agcgcagcgc atatagcacc agaccgatag tcagcgccag ccctgccaac
    89701 acggcacagg caatccacaa gcggttttta cggcgaatat tcatgcagcc tcctgctgtt
    89761 gcgcagcacg taaacgcgcc tcacgcgccc gctgttgcgc cacaccacgc agaattgcgc
    89821 gatgctgcat caccgagtgc acgaccaaaa ccaccagcgg aataacggtc atcaccaccg
    89881 ccagccagac aaaaaaggcg taaccgccca ttgcgaaaaa ttcattccag gaagcaaatg
    89941 caggggtcat ttacggcctc ttttcagtat cagttcactc acccacggac ggcgtttttc
    90001 catcagcaaa atcaaattac gcatccgcat cagcgtcagc gtggcagaga ggagcaggaa
    90061 gccaaaaatc gaccagcgca gcggcgaacg catcgccgga tcgatacttt gctgcatccg
    90121 cgttgatccc tgatgcaggg tgttccacca ctccacggag taatgaataa tcggcagatt
    90181 caccacgcca atcagcacca ggatacctgc cgcacggccc gccagacggc ggtcgtcgaa
    90241 ggcgtgccac agggcaatca cacccacata caaaaacagc agcaccagtt cagaagtcag
    90301 acgtgcatcc catacccacc aggtgcccca catcggtttt ccccatgcag agccggtaac
    90361 cagggcaata aaggtaaaca cggcaccaat gggggccatc gccgccaccg ccaggttggc
    90421 cattttcatc tgccagacaa ggccaataaa cgccgccacc gccattgatg cataaatgcc
    90481 catcgaccag atcgccgcag gcacatgcag gtagataatg cggtagctat ttccctgctg
    90541 ataatcagcc ggagcaaagc cgaatcccca gatccagccg acggtaagca cgactacact
    90601 ggcaattgcc agccacggta taaaccagcc acagatttga tacagccgtg gtgggatcgc
    90661 cagttgatgc agtgttttcc acatagtttc gataccagac tcgaacaaaa atcagtaatc
    90721 cagcgttatt gaatgctgat tcgtaacgct gccgccgtcg caaaaggact taatgtcgcg
    90781 gtgcctgcca gcaacgcgcc taaaattgcc agatacccgt caacgggcaa atgcatagaa
    90841 gccgcgtcca tcgcggcggt ggcaaagatg agtaatggga tagtcagcgg taacaccagt
    90901 atgctgagca gcacaccacc gcgcttaagt cccactgtca gcgccacgcc cggtgcaccg
    90961 agaaagccaa gcgtaggcgt tcccagcagt agcgttaacg ccatcacttg ccagccataa
    91021 acatccattc ccagtagcat tgctaccagt ggcgaaagga tgagtaacgg cagaccggtt
    91081 accatccagt gcgccatcac cttcgccagc acaacggcgg gcaagggtaa cggcaacaac
    91141 atcaattgtt caagactgcc gtcctgcaaa tcgtcacgga acagtcgttc cagcgccagc
    91201 aaggatgaaa gcagcgcggc aacccagata atgcccggtg caatacgtgc cagcagttgc
    91261 ggctccggac cgatactgag cggaaaaagg gtaattacaa tcaggaagaa ccacagcggg
    91321 ttggcgattt cagcgctatg gcgaaacgct acgcgcagct caagacggaa aatgcgccag
    91381 aacatcatac ggccctcgtt tgcgtcagtg aaatgcggcg aattttactt tcagcaacgt
    91441 tgagcggctg gtgggtagtg agaatcacaa tccccccctg ctctgtatgc tgcgccatac
    91501 gctgggtcag acgatcgacg ccgttaacgt caatcgcggt aaaaggctcg tcgaggatcc
    91561 ataacgtggc acgggtcagc cacagacgcg ctaaagcgac gcggcgttgt tgcccggcag
    91621 aaagttgatt taccggaata tcttcgaatc cggcaagccc ggcctgcgcc agggcttcca
    91681 gacattgtgc ggtatcgcca tcgcgatgat aaaagtgcag attttctaac gccgtcagcc
    91741 gggttttgat ccccggctga tggcctatcc ataacaggtt ttgatggtag ctgtcgcgta
    91801 cctgatgcaa gggctgccct tgccagagaa cctcgcctgc gtcagggcga gacaaccccg
    91861 tcagcaaacg gagaagcgtt gtcttccccg cgccgttgct accggtgatt tgtacccact
    91921 ctcctgcgtt cagcgtaaat gacaagccac taaataaggt tcgttcatcc cgctcacaaa
    91981 gtaactctct gacttcaagc atacccacgc aataaccctg ttaaaaacct ggctcgactt
    92041 cacgcatatc gggcagcttg tgcgctatcc ctttatggca atcaatacag gtttgcccat
    92101 ctttcaccgc ctggtcatgc atcttcgcgg caaccgattt ctgggcggtt gtatccatat
    92161 actcgaagtt gtgacagtta cggcactcct gcgagttatt gtccttcatg cgccgccact
    92221 cattctgtgc catcgtcaga cgatgagctt caaatttctg cggcgtgtca ataacgccaa
    92281 aaattttacc atacagctct ttacttgctt tgagcttgcg tatcatcttc ggcacaaact
    92341 cgtgcggaac gtgacaatcc ggacaggtcg cacggacgcc gctacggttg ttgtagtgca
    92401 cggaatccat gtattcctga tacaccgtgt tacgcatttc gtggcagcta atgcagaact
    92461 cttcggtatt ggctttttcc atcccagtgt taaagccgcc ccagaagacg atgccgccga
    92521 caaaaccgat caacagcagc gtccccagcg ccagacggct gggggtacgc caccatttcc
    92581 acaggcgctt aatcagacca ggcttacggt cagaatttcc cataataacc tcttatttcc
    92641 cgtaaccttt tgatggggta aaggtattcc ccacgattgg cgcggtatcg gcctgcggta
    92701 cgtgacattg cagacagaaa taacgacgcg gagccacttc cgcgcccact ttgccgtcgc
    92761 tgtccataaa gtgagtagga ctgatacgcg gcgcgccagt ggtgcgatag ctttcgacac
    92821 cgtggcactg caagcagcgg ttggtattgg tcgttacctg ataaccttca acgctatgcg
    92881 ggatcattgg cggctgattc acatagttca gcggcatccg atcctgctct tttggcatcc
    92941 gaatggcccc ttcctgcgtc ccggatactt ccggcgactg gctaaaatcg acgccattag
    93001 ccgcccaaac cgcgccgctt accaccaggg ccagcatcgc cgtccattga cacagcgctt
    93061 tcttcaggtc atggcttttc atgatttcgc tcccgaactc catcgtgtag ttattgtaaa
    93121 tacatcctca gaacagacat ccacgcagcg accgcacgtc atgcaatcgc ggctggtgac
    93181 ctgcaccggg ctttgttcat ccagcaccgg ggcacgtagc acatgcggtt ccgggcaaac
    93241 atgaaaacaa tccatacagc ggttacattt ctgccgatcg ctggccgcaa cggtaatcac
    93301 acctttgcta cccagcacgc catacagcgc gccaaccgga caaatgtgcc cgcaccagcc
    93361 gtgttcaacg accagtagat caaataaaaa cagcgcgaga ataagcagcg cgccgctgcc
    93421 gaagcccatt accaggctac gccccatcag ggaaacgggg ttgatccatt cccagatgag
    93481 cgtgccagtt aaggctgatc ccaccagaat gaccaccaac agcacgtagc gaatgtggcg
    93541 agggatcgtc gcagactgat tgaggtcaaa gcgcctgcgt aaccagttcg ctaagtcggt
    93601 aatcgggttc agcgggcaga cccagctgca aaataatcgc tttcctgcca gggcgtagag
    93661 cacggtgata atcaccgcac cggtcaacgc caccgtggca ggcagatgac cactggcaag
    93721 actttgcagc gtcatcagcg gatcggtcag cggaacggtg tcgaataaca ggctactgct
    93781 atagttgccg tgcaagatcc acacaccaaa ccacggaccg ctcaaaaaca tccccagcac
    93841 gaagaactga caaaggcgac gtaacaccag ccaacggtga ctgcgccacc agcctttttt
    93901 ctccagcgcc tcgcgcccgg cgtcacgttt acgatttgcc attgttcccc tccagccagc
    93961 cgaagcggta atggtgacct aactcccctt tcgccagtga cagcggtaac accttgattg
    94021 ccggttgttc cagcacgcat actttttcgc acttaccgca accggtacag gcgtcgctgt
    94081 gaaccgtcgg cagaaagcgg gcgtgcttac cggtacgcgt gttgcgctcc agctccaggg
    94141 tgatggcctc atcaattttc gggcattcgc gataacaaac atcgcagcgc agcccctgaa
    94201 agttgagaca gttttcctgg tccaccagta ccaccagccc catccgcgcg tcgtcgatcg
    94261 attcaatctc acgatccagc gcaccgctcg ggcacacttt ggcgcacgga atgtcctcac
    94321 acatttcgca aggaatatcc cgtgcgacaa aatatggcgt gcccgccgac aggccagagg
    94381 ccagcgtcgc cagttttaag gtgtcgtaag ggcaagcctg aacacactga ccgcaacgca
    94441 cacaggcact ggcaaaggcg ttctcgttta tggctccggg cgggcgcaac cgcacgccag
    94501 atgcgcgtgc ggtttgctgt tgtaacccca gcgccacacc cacggcagcc agcccgcctg
    94561 ctgtgcgaac gacatcgcgc agaaagcggc ggcgaccatt ttgaggtttc gctgaccggg
    94621 acataatggc ttacaccttc tccagtttga ccgcgcactt cttgaagtcc gtctctttcg
    94681 agagcggatc ggtcgcgtcc agcgttagtt tgttaaccag ctgtgcggcg tcgaagaacg
    94741 gcatgtacac caggccctgc ggcggacggt tacgaccgcg cgtttcgaca atcgagatca
    94801 cttcgccacg gcgagaaacc actttcactt tgtcgccacg gcgcagatcg cgcgctttcg
    94861 catccagcgg gtgaataaac aggaccgctt ccgggaaggc acggtgcagt tccggcacac
    94921 ggcgagtcat actgccggtg tgccagtgct ccaggacgcg tccggtagag agccacaggt
    94981 cgtactcttc atccggtgct tccgccgccg gttcgaacgg cagcgcgaag atcaccgctt
    95041 taccatccgg tttaccgtag aacttatagc cttcgcccgc tttcacgtac gggtcgttac
    95101 cttcgctgta acgccactgc gtttctttac cgttcaccac cggccagcgc agaccgcgcg
    95161 ctttgtggta gtcatcgaac ggcgcgagat cgtgaccgtg accgcgacca aaccatgcgt
    95221 actcttcgaa cagccctttt tgcagataga agcccagctc gcgggattca tcgttcagct
    95281 gatcttccgc cagttcggat accgggaatt tgctcacttc gggggtggca tacagaactt
    95341 cgtacagcgt tttgccacgc agttccggtt tcttcgccag cagctcttcc ggccatactt
    95401 cttcagtttt gaagcggcgg gagaactgga ctaactgcca gaggtccgat ttcgcttcgc
    95461 ctggcgcctg tacctgctga cgccagaact gagtacggcg ttcggcgtta ccgtaagccc
    95521 cctctttttc tacccacatt gcggtcggca ggatcaagtc ggcggccagc gcactgactg
    95581 tcggatacgg atcggagacg atgatgaagt tgcgcggatc gcgccagccc ggcatacgct
    95641 cttcattaat gttcggcccg gcctgcatgt tgttggtaca catggtccag taaacattca
    95701 gcttgccgtc tttcagcgca cggtcttgtg ctaccgcatg cagaccgatt ttcgccggaa
    95761 tggtgccgct cgggatattc cacttcttct cgcagatatc acgatgtttc tcgttagtta
    95821 ccaccatgtc cgcaggcaga cggtgagcaa aggtgcccac ttcacgcgca gtaccacacg
    95881 cggaaggctg cccggtcagg gagaacggac cgcaacccgg ctgggaaatt ttgccggtca
    95941 gcaggtgcag gttgtagacc aggttgttgg cccacacgcc acgagtatgc tggttgaagc
    96001 ccatcgtcca gtaggagatg actttcttgt tcggatcggc atacagctgc gccagttgtt
    96061 ccagctgatc ttttggtacg ccagtcattt cagcggtttt ttccagcgta tactcagcaa
    96121 cgaaggcttt gtaatcttca aagctcatcg gttcggaggc gtcagaaccc ggattcttcg
    96181 ctgctttttc cagcggatgg gtcggacgta aaccgtagcc gatgtccgtc gcccctttgc
    96241 gcaggttaac gtgcttgctg aagaagtcct gatttatcgc attgttttga atgatatagt
    96301 tggcgatgta gttcaggatc accaggtcag attgcggcgt aaagatgatg ccgttatccg
    96361 ccagctcgaa gctacgatgc tggtaggttg aaagcaccgc cacggtgacg tcctggttag
    96421 agagacgacg gttagtgatg cgtgaccaga ggatcgggtg catctccgcc atgtttgcgc
    96481 cccacagcac aaacgcgtca gcctgctcga tgtcgtcata gcagcccatc ggctcatcca
    96541 taccaaaggt tcgcataaag ccaactactg ccgacgccat acagtgacgc gcattcgggt
    96601 cgatgttgtt cgaacggaag cccgctttaa acagcttgga cgcggcataa ccttcccaga
    96661 tagtccactg accagaaccg aacataccga tcgattccgg ccctttttct ttcagggcgg
    96721 ttttgaattt ctcttccatc acatcgaagg cctgatccca ggtgattggg gtaaattcgc
    96781 cttctttgtc atatttaccg tttttcatac gcagcagcgg ctgcgtcaaa cggtctttac
    96841 cgtacatgat cttgggcagg aaatagccct taatgcagtt caggccacgg ttaaccggtg
    96901 cgtccgggtc gccctgacag gccaccacac gcccctgctg cgttccgacc agaacgccgc
    96961 aaccagtacc gcagaaacgg cacggcgctt tatcccattt aatggcttcc tgctgaccaa
    97021 caacggcgcg ggcaacgccc ggcacgctga gaccggcagc cgccgcagcg gccgcaacgg
    97081 cgttagcttt cataaagcta cgacgactga gtttcatggt gtttcctcac cttgctcttc
    97141 ctgctggtga taaaccagcg acaccgccag cacgccctct acgttgcgta ctgactcaat
    97201 ggtttggatc agcgtttcgc tgtcttctgc ttccaccacc acaatcaact gaccgctcgg
    97261 cgcgtcgctg acagcaactt cacagccggg aaaggcgttc agttgggtgc tgatgtctga
    97321 aattcgttcg cttttggcct gcacaaccag gctgcaaact tgccagttag tgtgcatgga
    97381 gatactccgc agttatggct gataccggac agctggctgc acatgctcca catccgttgc
    97441 aaagttggct attaagttgc ggctggtaga tcccggagag cgtcgggcga aagataattg
    97501 ccattggctc acagctatcc tgacagcggc ggcattcaac tgactgatac gccaggcacg
    97561 cgtccccaat ggtgaactgc aaatcccagg ccctggtgtg gcgcggagaa aatagcgatt
    97621 cggggcaagc ctgcgcgcag gcgtaacaga agctgcactc gttatttttg aaattaacgc
    97681 tcggatagcc gcccgcgccg cgttgcagaa tgttgttttc acaagcatta atacaggcgt
    97741 cgcagcgggt gcaatgggtc agaaaatgag attcatcacc ggaccagggc ggacggatac
    97801 cgttactggc tttgcgccag cgaccagtga gtatgccccg acgggatgca tcaatcttca
    97861 cattgacctt ccatcattaa cgcgctctga aaattgagag cgaccaaata aaccgcataa
    97921 ttaataagcc atttttatag ccgctaagat attaaaggat gtgtcaaaga tgcatacccc
    97981 gatcgaggta aaacctgtag caggatcaaa agagtggcgg gaagcgtggc aaaaacgggc
    98041 ttttgctcac atttcaaatg gttataaata tatttatata gcgattaatt caccagagat
    98101 atttctgctg gtttgctctc
//
LOCUS       DAZFKY010000002        69383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFKY010000002 DAZFKY010000000
VERSION     DAZFKY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..824
                     /gene="ynaI"
                     /locus_tag="ACHKCL_000099"
     CDS             <1..824
                     /gene="ynaI"
                     /locus_tag="ACHKCL_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5761153.1"
                     /translation="KFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITS
                     ARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIM
                     LYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPG
                     RMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNI
                     MVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(875..2488)
                     /gene="mppA"
                     /locus_tag="ACHKCL_000100"
     CDS             complement(875..2488)
                     /gene="mppA"
                     /locus_tag="ACHKCL_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5761154.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2824..3723)
                     /gene="pgrR"
                     /locus_tag="ACHKCL_000101"
     CDS             complement(2824..3723)
                     /gene="pgrR"
                     /locus_tag="ACHKCL_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5761155.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3861..4781
                     /locus_tag="ACHKCL_000102"
     CDS             3861..4781
                     /locus_tag="ACHKCL_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5761156.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4781..5711
                     /locus_tag="ACHKCL_000103"
                     /pseudo
     CDS             4781..5711
                     /locus_tag="ACHKCL_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            5732..6370
                     /locus_tag="ACHKCL_000104"
     CDS             5732..6370
                     /locus_tag="ACHKCL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5761157.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            6661..7389
                     /gene="mpaA"
                     /locus_tag="ACHKCL_000105"
     CDS             6661..7389
                     /gene="mpaA"
                     /locus_tag="ACHKCL_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5761158.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7364..8329)
                     /gene="ycjG"
                     /locus_tag="ACHKCL_000106"
     CDS             complement(7364..8329)
                     /gene="ycjG"
                     /locus_tag="ACHKCL_000106"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5761159.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            8448..8954
                     /gene="tpx"
                     /locus_tag="ACHKCL_000107"
     CDS             8448..8954
                     /gene="tpx"
                     /locus_tag="ACHKCL_000107"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5761160.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(8998..10539)
                     /gene="tyrR"
                     /locus_tag="ACHKCL_000108"
     CDS             complement(8998..10539)
                     /gene="tyrR"
                     /locus_tag="ACHKCL_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5761161.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10687..11748)
                     /gene="ycjF"
                     /locus_tag="ACHKCL_000109"
     CDS             complement(10687..11748)
                     /gene="ycjF"
                     /locus_tag="ACHKCL_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5761162.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11745..13142)
                     /gene="ycjX"
                     /locus_tag="ACHKCL_000110"
     CDS             complement(11745..13142)
                     /gene="ycjX"
                     /locus_tag="ACHKCL_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5761163.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13297..14295
                     /gene="ycjW"
                     /locus_tag="ACHKCL_000111"
     CDS             13297..14295
                     /gene="ycjW"
                     /locus_tag="ACHKCL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5761164.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(14406..15311)
                     /gene="ompG"
                     /locus_tag="ACHKCL_000112"
     CDS             complement(14406..15311)
                     /gene="ompG"
                     /locus_tag="ACHKCL_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5761165.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15356..16438)
                     /locus_tag="ACHKCL_000113"
     CDS             complement(15356..16438)
                     /locus_tag="ACHKCL_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5761166.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16452..17111)
                     /gene="pgmB"
                     /locus_tag="ACHKCL_000114"
     CDS             complement(16452..17111)
                     /gene="pgmB"
                     /locus_tag="ACHKCL_000114"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5761167.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(17108..19375)
                     /gene="ycjT"
                     /locus_tag="ACHKCL_000115"
     CDS             complement(17108..19375)
                     /gene="ycjT"
                     /locus_tag="ACHKCL_000115"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO5761168.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(19372..20427)
                     /gene="ycjS"
                     /locus_tag="ACHKCL_000116"
     CDS             complement(19372..20427)
                     /gene="ycjS"
                     /locus_tag="ACHKCL_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5761169.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20437..21225)
                     /gene="ycjR"
                     /locus_tag="ACHKCL_000117"
     CDS             complement(20437..21225)
                     /gene="ycjR"
                     /locus_tag="ACHKCL_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5761170.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(21243..22295)
                     /gene="ycjQ"
                     /locus_tag="ACHKCL_000118"
     CDS             complement(21243..22295)
                     /gene="ycjQ"
                     /locus_tag="ACHKCL_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5761171.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22326..23168)
                     /gene="ycjP"
                     /locus_tag="ACHKCL_000119"
     CDS             complement(22326..23168)
                     /gene="ycjP"
                     /locus_tag="ACHKCL_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5761172.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23155..24036)
                     /gene="ycjO"
                     /locus_tag="ACHKCL_000120"
     CDS             complement(23155..24036)
                     /gene="ycjO"
                     /locus_tag="ACHKCL_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5761173.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(24057..25349)
                     /gene="ycjN"
                     /locus_tag="ACHKCL_000121"
                     /pseudo
     CDS             complement(24057..25349)
                     /gene="ycjN"
                     /locus_tag="ACHKCL_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(25363..27041)
                     /gene="ycjM"
                     /locus_tag="ACHKCL_000122"
                     /pseudo
     CDS             complement(25363..27041)
                     /gene="ycjM"
                     /locus_tag="ACHKCL_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(27251..27565)
                     /gene="pspE"
                     /locus_tag="ACHKCL_000123"
     CDS             complement(27251..27565)
                     /gene="pspE"
                     /locus_tag="ACHKCL_000123"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5761174.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27640..27861)
                     /gene="pspD"
                     /locus_tag="ACHKCL_000124"
     CDS             complement(27640..27861)
                     /gene="pspD"
                     /locus_tag="ACHKCL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5761175.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27870..28229)
                     /gene="pspC"
                     /locus_tag="ACHKCL_000125"
     CDS             complement(27870..28229)
                     /gene="pspC"
                     /locus_tag="ACHKCL_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5761176.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28229..28453)
                     /gene="pspB"
                     /locus_tag="ACHKCL_000126"
     CDS             complement(28229..28453)
                     /gene="pspB"
                     /locus_tag="ACHKCL_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5761177.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28507..29175)
                     /gene="pspA"
                     /locus_tag="ACHKCL_000127"
     CDS             complement(28507..29175)
                     /gene="pspA"
                     /locus_tag="ACHKCL_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5761178.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29342..30319
                     /gene="pspF"
                     /locus_tag="ACHKCL_000128"
     CDS             29342..30319
                     /gene="pspF"
                     /locus_tag="ACHKCL_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5761179.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30450..31715)
                     /gene="puuE"
                     /locus_tag="ACHKCL_000129"
     CDS             complement(30450..31715)
                     /gene="puuE"
                     /locus_tag="ACHKCL_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO5761180.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(31753..33033)
                     /gene="puuB"
                     /locus_tag="ACHKCL_000130"
     CDS             complement(31753..33033)
                     /gene="puuB"
                     /locus_tag="ACHKCL_000130"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO5761181.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     GVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(33035..34522)
                     /gene="puuC"
                     /locus_tag="ACHKCL_000131"
     CDS             complement(33035..34522)
                     /gene="puuC"
                     /locus_tag="ACHKCL_000131"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO5761182.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34672..35229)
                     /gene="puuR"
                     /locus_tag="ACHKCL_000132"
     CDS             complement(34672..35229)
                     /gene="puuR"
                     /locus_tag="ACHKCL_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO5761183.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35256..36020)
                     /gene="puuD"
                     /locus_tag="ACHKCL_000133"
     CDS             complement(35256..36020)
                     /gene="puuD"
                     /locus_tag="ACHKCL_000133"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO5761184.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36232..37650
                     /gene="puuA"
                     /locus_tag="ACHKCL_000134"
     CDS             36232..37650
                     /gene="puuA"
                     /locus_tag="ACHKCL_000134"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO5761185.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37799..37963
                     /locus_tag="ACHKCL_000135"
     CDS             37799..37963
                     /locus_tag="ACHKCL_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761186.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            37953..39338
                     /gene="puuP"
                     /locus_tag="ACHKCL_000136"
     CDS             37953..39338
                     /gene="puuP"
                     /locus_tag="ACHKCL_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO5761187.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            39472..39717
                     /gene="ymjA"
                     /locus_tag="ACHKCL_000137"
     CDS             39472..39717
                     /gene="ymjA"
                     /locus_tag="ACHKCL_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO5761188.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            40030..41673
                     /gene="sapA"
                     /locus_tag="ACHKCL_000138"
     CDS             40030..41673
                     /gene="sapA"
                     /locus_tag="ACHKCL_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5761189.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLTHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41670..42635
                     /gene="sapB"
                     /locus_tag="ACHKCL_000139"
     CDS             41670..42635
                     /gene="sapB"
                     /locus_tag="ACHKCL_000139"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5761190.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42622..43512
                     /gene="sapC"
                     /locus_tag="ACHKCL_000140"
     CDS             42622..43512
                     /gene="sapC"
                     /locus_tag="ACHKCL_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5761191.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43512..44504
                     /gene="sapD"
                     /locus_tag="ACHKCL_000141"
     CDS             43512..44504
                     /gene="sapD"
                     /locus_tag="ACHKCL_000141"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5761192.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44506..45312
                     /gene="sapF"
                     /locus_tag="ACHKCL_000142"
     CDS             44506..45312
                     /gene="sapF"
                     /locus_tag="ACHKCL_000142"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5761193.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(45364..46527)
                     /locus_tag="ACHKCL_000143"
     CDS             complement(45364..46527)
                     /locus_tag="ACHKCL_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO5761194.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(46536..47909)
                     /locus_tag="ACHKCL_000144"
     CDS             complement(46536..47909)
                     /locus_tag="ACHKCL_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO5761195.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(47913..51015)
                     /locus_tag="ACHKCL_000145"
                     /pseudo
     CDS             complement(47913..51015)
                     /locus_tag="ACHKCL_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(51015..52136)
                     /locus_tag="ACHKCL_000146"
     CDS             complement(51015..52136)
                     /locus_tag="ACHKCL_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5761196.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(52285..52851)
                     /locus_tag="ACHKCL_000147"
     CDS             complement(52285..52851)
                     /locus_tag="ACHKCL_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5761197.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            53329..54117
                     /gene="fabI"
                     /locus_tag="ACHKCL_000148"
     CDS             53329..54117
                     /gene="fabI"
                     /locus_tag="ACHKCL_000148"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5761198.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            54261..55388
                     /gene="yciW"
                     /locus_tag="ACHKCL_000149"
     CDS             54261..55388
                     /gene="yciW"
                     /locus_tag="ACHKCL_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5761199.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            55456..57390
                     /gene="rnb"
                     /locus_tag="ACHKCL_000150"
     CDS             55456..57390
                     /gene="rnb"
                     /locus_tag="ACHKCL_000150"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5761200.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(57509..57571)
                     /locus_tag="ACHKCL_000151"
                     /pseudo
     CDS             complement(57509..57571)
                     /locus_tag="ACHKCL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            57625..59610
                     /gene="pdeR"
                     /locus_tag="ACHKCL_000152"
     CDS             57625..59610
                     /gene="pdeR"
                     /locus_tag="ACHKCL_000152"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5761201.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(59592..59720)
                     /gene="ymiD"
                     /locus_tag="ACHKCL_000153"
     CDS             complement(59592..59720)
                     /gene="ymiD"
                     /locus_tag="ACHKCL_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5761202.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            59758..59931
                     /gene="yciZ"
                     /locus_tag="ACHKCL_000154"
     CDS             59758..59931
                     /gene="yciZ"
                     /locus_tag="ACHKCL_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5761203.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            60021..60770
                     /gene="yciT"
                     /locus_tag="ACHKCL_000155"
     CDS             60021..60770
                     /gene="yciT"
                     /locus_tag="ACHKCL_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5761204.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            61039..61257
                     /gene="osmB"
                     /locus_tag="ACHKCL_000156"
     CDS             61039..61257
                     /gene="osmB"
                     /locus_tag="ACHKCL_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5761205.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(61383..61709)
                     /gene="yciH"
                     /locus_tag="ACHKCL_000157"
     CDS             complement(61383..61709)
                     /gene="yciH"
                     /locus_tag="ACHKCL_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5761206.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(61709..62446)
                     /gene="pyrF"
                     /locus_tag="ACHKCL_000158"
     CDS             complement(61709..62446)
                     /gene="pyrF"
                     /locus_tag="ACHKCL_000158"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5761207.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(62639..63808)
                     /gene="lapB"
                     /locus_tag="ACHKCL_000159"
     CDS             complement(62639..63808)
                     /gene="lapB"
                     /locus_tag="ACHKCL_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5761208.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(63815..64123)
                     /gene="lapA"
                     /locus_tag="ACHKCL_000160"
     CDS             complement(63815..64123)
                     /gene="lapA"
                     /locus_tag="ACHKCL_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5761209.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(64272..65036)
                     /gene="pgpB"
                     /locus_tag="ACHKCL_000161"
     CDS             complement(64272..65036)
                     /gene="pgpB"
                     /locus_tag="ACHKCL_000161"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5761210.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            65206..65796
                     /gene="ribA"
                     /locus_tag="ACHKCL_000162"
     CDS             65206..65796
                     /gene="ribA"
                     /locus_tag="ACHKCL_000162"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5761211.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(65860..68535)
                     /gene="acnA"
                     /locus_tag="ACHKCL_000163"
     CDS             complement(65860..68535)
                     /gene="acnA"
                     /locus_tag="ACHKCL_000163"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5761212.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(68699..68794)
                     /gene="ymiC"
                     /locus_tag="ACHKCL_000164"
     CDS             complement(68699..68794)
                     /gene="ymiC"
                     /locus_tag="ACHKCL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5761213.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(68908..69075)
                     /gene="yciX"
                     /locus_tag="ACHKCL_000165"
     CDS             complement(68908..69075)
                     /gene="yciX"
                     /locus_tag="ACHKCL_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5761214.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(69078..69218)
                     /gene="ymiA"
                     /locus_tag="ACHKCL_000166"
     CDS             complement(69078..69218)
                     /gene="ymiA"
                     /locus_tag="ACHKCL_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5761215.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        16901 a        18316 c        16983 g        17183 t
ORIGIN      
        1 tcaaatttat cactccaggc gttatcgatt ttatctgtac gtccctgatt gcggttattc
       61 tgacgattaa gctatttttg ctgattaatc agtttgaaaa acagcagatt aaaaagggtc
      121 gcgatatcac cagtgcgcgg attatgtcgc gcattatcaa aatcaccatt attgtggtgc
      181 ttgttctgct ttatggcgaa catttcggca tgagcctttc tggcttgctg acctttggtg
      241 gtattggtgg tctggctgtc ggtatggctg gcaaagatat tctgagtaat ttcttttccg
      301 ggatcatgct ctatttcgac cgtcctttca gtattggcga ctggatccgt tcaccggaca
      361 gaaatatcga aggtacagta gcggaaattg gctggcgaat taccaaaatt acgacctttg
      421 ataatcgtcc attgtacgta ccgaactcgc tgttttcatc gatcagcgta gaaaacccag
      481 gacgaatgac caaccgccgc attaccacga ccattggttt acgttatgag gatgcggcaa
      541 aagtgggcgt tatcgtcgaa gccgtacgtg agatgctgaa aaatcacccg gccatcgacc
      601 agcgacaaac cttactggtt tatttcaacc agtttgctga ctcgtcattg aatattatgg
      661 tttattgctt taccaaaacc acggtatggg ctgagtggct tgctgcacag caagacgttt
      721 atttgaagat tatcgatatt gtacagtcac acggcgcgga ctttgccttc cccagccaga
      781 cgctgtatat ggataacatt acaccgccgg aacagggtcg ctaaatcaga catcactggc
      841 gttatccaca agggtaacgc cagttcccat cacatcaatg cttcacaata tacatagtcc
      901 gactgtacgc cacatcctca gggttattaa tgggataacc tttcagccac ggtttgatta
      961 atcgtccatt ggtatattga taaattggtg caatcggtgc ttgctccatg aggatttttt
     1021 ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt ttccgtcgat gcctgggcca
     1081 gaactttgtc gtatgccgga ttgttaaagc gtgaaatatt tcctgaatgc gttgacgtta
     1141 ataatgtcag gaaagtggag ggttcattat aatcccccac ccatgaggcg cggataacat
     1201 caaaattgcc ggtgttacgg ctatcgatat aggttttcca ttcctgattt tgcaatttaa
     1261 catctacgcc aaggttcttt ttccacatcg atgctacagc aatcgcaatt ttttgatggt
     1321 tttctgaagt gttatacaaa agcgtcagct tcagcggttt ttgcggacca taaccagctg
     1381 cgctcaacaa agtttttgcc tgcgcattca gttcttcctg actcatttgt tcaaacggcg
     1441 aaggttccgg cgtaaatccg gcggtaacat ctggtgtaaa atgccacgct ggcttttcgc
     1501 ccgtccctaa tactttttcg gtcatcaggc ggcgatctat cgtcatactt aatgccagac
     1561 gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa cgcataataa taggtcccga
     1621 gctgcggcgg cgtataaacc tgccccggaa tatccttcaa cagcttctga tacatatttt
     1681 tcgggaagga ttcggtgata tcaatatccc ccgcgaggta acgcttagtg gctgcggatt
     1741 cctgattaat tggcaggaag gtcacttttt gcagtaccgt tttggcgtta tcccaataat
     1801 gggtattcgg taccacgacc agtttttcat tgactacgcg ctctttaaga acataagcgc
     1861 cattgccgat cagatttccg ggtttcgtcc actctttacc gctttctacg ttggcttttt
     1921 gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa ccacggcaac ggtttatcaa
     1981 gctgaatttt caaagtatgg acatcaactg cggtgacgcc aagctggtca ggtgtagctt
     2041 taccatcaat aatcgcctgt gcgttgttga ttcccgccag cgcggcaaac catgcaaatg
     2101 gcgatagcgt tttcgggtcc accagacgct gccagctgta gacaaaatct tgcgccgtta
     2161 ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt aaaagtccag atacggttgt
     2221 cattactttt ccactgagtc gcaacgccgg ggacaatctc ccctttctcg ttctgattca
     2281 ccagtccttc aaacagatcg cgaatgacct gaatctctgg caggcccacg gctttagcgg
     2341 gatccagcga cgcaggctca tctttaatgt ggcgcaccag ctcctgcttc tctgccagta
     2401 ctgtgccgct cggaacttct gcagcatacg aaagagaaat gctgctgacc aacagcgcac
     2461 aacacgtgac tgaaacagag tgcttcataa gtttccctct aaccgacatt aaaattgatg
     2521 cgtaattatt tgtttcgcct taaagaaatg caaacaactt ccatcagaaa gtgacacagg
     2581 aagcataaaa tgttttatgt atcaatatat taaatcagat ttagtgtaaa taatagacgc
     2641 caaaatgcgg caaagcacat agaacaccga acactacgtt cgggcttcac acgttcaaac
     2701 cagttttcgg tatcataatc agcatctgtc gccattgtac gaagaataaa aaataaccaa
     2761 aacgttgaaa ggaactgaga atacgttaag agaagaatta aattaatgta tcagcaagta
     2821 gtgctaaaca gcacctttat acttcagcct atctataaat aatgagaatg ccgagccagc
     2881 atgtcgacgg tgtggatagt acaggtgata accgggtaaa tctggtgtga atttatccaa
     2941 aacacgtatc agtttttttt ctttaatcgc ccgctcaacc tgatcataag gtagatacgc
     3001 caatccatgc ccatcaattg cagcatcaat gatcaggtct atcgtattca gtaaaagctg
     3061 accttccatg cgaacacgaa cttcacgtcc accgcgtatt aatctccagc gatttgctgt
     3121 acccgatgtg ggaagataca aattaattgc ctgatgatct attaattgtg acactgacgt
     3181 tggaacactt cggcgagaaa aataatccgg tgagccaaca atagccattg gtatatctgg
     3241 cccgattcga atggcgatca tatctttatc catctcccca cccagacgga cgcctgcatc
     3301 aaaacgttca gaaacgacat cagtcaaacc ataatcaatg ataagctgaa tatcaatttc
     3361 agggtgtgat ttcaggaatg tgcgcatggc tggtaacaat atcgtttttg ctgcatgttc
     3421 tacagtagta atacgtattg tcccggatgg gcggttctgc agatcgctca gggatgccag
     3481 ggcagaatct atgtcatgca gcatagggcc aagaacagac aaaagatgct cgcccgcttc
     3541 agtgggtaca acactgcgcg tggttcgcgt cagaagccgc aatcccaatc gttcttctat
     3601 acggcgcact atctggctta aagctgactg cgccatgctc aggcgggctg ctgcgcgagt
     3661 gaagctacgc tcctctgcaa cgacgacaaa cgccatcaga tcagcgatct cttcacgctt
     3721 cattcagtat ctccatacaa tctattaatc atttaaatga taggtctaat catgatttag
     3781 ggtctaatca acttaatgat caatatctat agtgatatca ccaaccgtta aaacgaacca
     3841 acaactccat gaggaacgga atgatgaata ataaagtcag cttcactaac agcaataatc
     3901 caaccatctc tttgtctgca gtgatctatt ttcctccaaa atttgatgaa acccgtcaat
     3961 atccggcaat tgtggtatct cacccaggtg ggggcgttaa agaacaaacg gccgggacct
     4021 atgccgaaaa actggcggaa aagggatttg tcactattgc ttatgacgca tcttatcagg
     4081 gtgaaagtgg cggcgagccg cgtcagttag aaaacccata cattcgtact gaagacgtca
     4141 gtgccgtgat tgattacctg acaacgcttt cttatgtcga caatacccga attggtgcga
     4201 tgggaatttg tgccggtgca ggatatacag ccaatgctgc aattcaggat cgccgcatca
     4261 aggccatcgg taccgttagt gcggtcaata ttggttcgat gttccgtaat ggttgggaaa
     4321 acaatgtgaa atctattgat gccctgccat acgttgaagc gggttcaaac gccagaacca
     4381 gcgatattag cagcggagaa tacgccatta tgccactggc accaatgaag gagtccgatg
     4441 cgccgaacga agaactgcgt caggcctggg agtattacca cacccctcgc gcgcagtatc
     4501 caacagcacc gggttacgct actttgcgca gccttaacca gattattacc tatgatgctt
     4561 accatatggc ggaagtgtac ctgactcagc cgacgcagat tgtggcgggc agccaggcag
     4621 ggagcaaatg gatgagtgac gacctgtacg atcgggcatc aagccaggat aaacgctatc
     4681 acattgttga aggcgcaaac catatggatc tttatgacgg caaagcctat gtggctgaag
     4741 caatttcagt attagctccg ttctttgagg aaactctgta atgaaacata ttcaaatccg
     4801 caatagtgat atggcctggc atattgctgc caatattcag tttccaccaa attttgatga
     4861 gtcaaaacag tatccggcga ttatcagcgt ccatcctttt ggcagttgta aggagcagac
     4921 gtctggcaat atctatggca aggcattggc tgaaaaaggc tatgtcgttc tcgcttatga
     4981 cgccagtttc cagggagaat ctggtgggga gcctcgttgg atagaagatc cgacacagcg
     5041 cgtagaggat attagccgtg ttatcgatta tgcagtaact ctgccatatg ttgatgctga
     5101 acgcattggc gtgctgggtg tgtgtggggg ggggtggcta tgccattaat gccgcgctga
     5161 cagaaaaacg aattaaagct gtagtgagta ttacgggtgt taatatcggg cgtctttttc
     5221 gggaaggttt cagcaattac gatccgattg gcgcattgaa tgcaatggcg tcgcaacgaa
     5281 ccaaagaggc tcgtggtgga gaattacaga tcaacgaatt acttcccgcc agcctggatg
     5341 cagcaaaagc ccatggactg accgaacgcg atgtttatga agcaactgac tactataaaa
     5401 caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt gttctcacat gcacagaaaa
     5461 cgctggcctg ggatgccttc gcatttactg aagtgttact gactcagccg gtgatggtag
     5521 tagttggtga aaaagtcggt gccttcggtg cttatcgtga tggactggaa gtctatggcc
     5581 gggcgatggt ttcgcaggat cgtcagttag tttcattacc cgatttctca cattatgaac
     5641 tgtatgacaa accggaagca gttcaggagg cgctggaaaa agttattcct tttttcaaca
     5701 cccatctgtg ataagtttca tgagaaatat tatgaaaaat gtactcattc ttggtgctgg
     5761 cggtcagata gcccgccatg tgattaatca actggcagat aaacaaacca ttaaacaaac
     5821 tttatttgct cgtcagccag caaaaataca caaaccctat ccaactaata gccagattat
     5881 tatgggggat gtactgaatc atgcagcact gaagcaggcc atgcagggac aggatattgt
     5941 gtatgccaac cttacggggg aagatttaga tattcaggca aattcagtga tcgctgcgat
     6001 gaaagcctgt gatgttaaac gtctgatctt tgttctttcc ctcggcattt atgatgaagt
     6061 tccaggaaaa tttggcgaat ggaataatgc agttattggt gagcccctaa aaccattccg
     6121 cagggctgcg gatgcaattg aggcttctgg actggaatat acaattcttc gccctgcctg
     6181 gctgacggat gaagatatta ttgattatga gcttacctca cgcaatgaac cattcaaagg
     6241 tacgatcgta tcacctaaga gtgtcgcagc attaattaca gatatcatcg ataaacctga
     6301 gaaacacatt ggtgagaata taggcatcaa tcaacccggg actgatggcg acaagccatt
     6361 cttcatgtaa atcaattata taaccaccat cgtactgtac tatgtgcagt acgatttatt
     6421 aaacttatta aggtgaactg atcaatcaga tgcgattaac atgtgtagaa ccagtaagaa
     6481 taaaatacgc catgtatata acattacgac aattctaata tctgcacatc aatgaaaata
     6541 caaacaactt ccggcaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat
     6601 ttgataatcc gcaaaatttg cacaacactg cctgtaatac ttcctgaacc agagacgatc
     6661 atgaccgtaa cccgcccacg cgccgaacgc ggcgcatttc cgcccggaac agaacattac
     6721 ggacgttcat tattgggcgc gccgttaatc tggtttccgg cccctgcagc cagtcgcgaa
     6781 agtggtttga ttctggctgg cactcacggt gatgaaaact cttcggtcgt cacgctctcc
     6841 tgtgctctgc ggactttgac accttctttg cgccgccatc atgtggtgct gtgtgtgaat
     6901 cctgacggct gccagctggg gttacgggcc aatgctaatg gtgtggattt aaacagaaac
     6961 tttccggcgg cgaactggaa ggaaggtgaa acggtttatc gctggaacag cgccgctgaa
     7021 gaacgtgatg tcgtattact gacaggtgac aaacctggct ccgaacctga aacccaggcg
     7081 ctgtgccagc ttatacaccg cattcaacct gcctgggtgg tctcattcca cgatccactg
     7141 gcctgtattg aagatcccag acacagcgaa ttaggcgaat ggctggctca ggcgtttgaa
     7201 ttgcctctgg taaccagcgt cggttatgaa acgcctggct ctttcggcag ttggtgtgca
     7261 gatctgaact tgcattgcat caccgcagag tttccgccca tctcctccga cgaagccagc
     7321 gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga tgcaattcgc
     7381 ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag ccaggtcggt ccgtcaagat
     7441 cggcgaaact gacctgcggc accagcggta aagcggcgct aatggcccga gaggtacaca
     7501 acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc ttcagtcgcc agcgccagcg
     7561 cttccgtcag acccccggtt ttatcgagct taatattaac catctcatag cgccctttca
     7621 gcgccttcaa attgctacga gtatgacaac tttcatcagc acaaatcggc aacggatgaa
     7681 taaaattctc cagcgccgca tcgtcctgcg caggaagcgg ttgttcaagc atcgcaacgc
     7741 ctaaatccgc caatagctgg caacgtgccg ccaacccttc tgcatgccag gattcatttg
     7801 catcaacgat cagcgtggta tcgggcacag ctgtgcgaat tgccaccatc cgctcactga
     7861 taagatggtt atccagcttc actttcagta atttcgcgcc ggcctgccag agtgttgatg
     7921 cactattggc catctgatca ggcgtaccga tgactaccgt ctgtgcagtg ataactgtct
     7981 cgggaagcgt tatgccgatc aaatcagcca gcgattgctg ctgttttcgc gcggccagat
     8041 cccacaatgc acaatccagc gcattacgtg ctgcgccggc agggagaatt ttttgcaact
     8101 cctcccgtgt cagccctttc tctaattgcg gcacgacgct cataatttgc gccattaccg
     8161 aggcatcact ttctccataa cgcggatacg gcgtgcattc gccggtgcct ttaataccct
     8221 cttcttccag ttcaaccacc accacgcgcg cttcactgcg acttccccgg gcaatcacaa
     8281 acggggtatg taagggccag gcttcctcaa ataccttaac ggttctcatc tttactcctt
     8341 ggtgtcgcgg tggtctgcgg attaagcgca aatagcgttt gctgtgttat tgacagttag
     8401 cataaactag gtgtgacgtt aactatatgt aaacaggaaa gataatcatg tcacaaaccg
     8461 ttcatttcca gggcaacccg gttacagtcg ccaattccat cccgcaggcg ggtagcaaag
     8521 cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt caccctcggt cagtttgcgg
     8581 gtaaacgcaa agtgctgaac attttcccga gtattgatac cggtgtttgc gccgcatcag
     8641 tacgtaagtt taaccaactg gcaaccgaga tcgacaacac cgttgtgctg tgtatctctg
     8701 ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga aggtctgaac aacgttatca
     8761 ccctctccac tttccgtaac gctgaatttc tgcaagccta cggtgtggca attgctgatg
     8821 gcccactgaa aggtctggca gcgcgtgccg ttgtggttat tgatgaaaat gacaatgtga
     8881 ttttcagcca gctggtggat gaaatcacca ccgagccgga ttacgaagca gctctggctg
     8941 tactgaaagc ataagttaat atgcctgatg gtgttgcacc atcaggcata ttcgcgctta
     9001 ctcttcgttc ttcttctggc ttaagccata ttcccgcaac ttattggcaa tcgcggtatg
     9061 tgaaacgccg agacgttttg ccagtttgcg cgtgctggga taattgcgat aaagctgggt
     9121 taataccgag cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca tcgcatcttc
     9181 gcccacggct accgttgcgg cgtcataatc cggcaacaaa atatcctgtg gacgcagctc
     9241 ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc tttaactgcc gcacatttcc
     9301 cggccacgca taacgcgtaa gtacagtatt caggtcagcg gccagtttcg gacgcggcac
     9361 gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt aacggcatga tgtcctgcgg
     9421 acagtcacgt agcggcggca ggttgagcgt caacacgttc agacgataat agagatcttc
     9481 acggaacatg cctttttgca ccagttcgac cagattcttc tgcgtagcgc aaatcacccg
     9541 cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat cattaaggaa
     9601 acgcagtaat ttcgcctgca tccgtggtga catctcacct atttcatcca acagcaccga
     9661 accaccgttc gcctgctcaa agaatccttt cttcccttcc ggagcatgac caaacagctc
     9721 actctcgacc gcatcttccg gtatagacgc acagttcagc gccaggtaag gtttgctcgc
     9781 tcttgggctt gcctgatggc aggcgtaggc aaaaagatct ttacctgtac ctgtgtcacc
     9841 cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc gcctgttcga caacatgctt
     9901 cattttcggg ctgacagcga caatttgact gaaggcgctg acgtcctggg cggcgacatt
     9961 ttgcaactga cggcccatac gaatcgttga tcgcaacatc accaccgcac ctgtcaggac
    10021 gtgttgatca ttttcatcct gaagataaac aggcgtaatc tccatcagga agttctgtcc
    10081 attaataaca acatgctcgt tatgggaatc ttgcggttcg ctttccagcc aacgtaaaaa
    10141 attaaagccg ttaatcaatt gtgcggcggt atggttgcgc agtcgatcca atttttgccc
    10201 aaaaagctga cagctcgccg ggttcgccat atccactttg cttttcatat cgacagagag
    10261 cacaggttca ggcaacgcct ccagtaacgc gctcaacgcc agatgctcac gttcggaagg
    10321 catccacggg acagtacgca catcggtaac acctgcaata cggcgtattt cggccatcag
    10381 actgctgaaa ctctcaaact ccagttcagc aaaattgagg tagattcgcc caatgggatc
    10441 aatctcaata ccgcgtaaat caatgcctct tagcacgagt agatcgagta attcgcgggt
    10501 cagaccgagt cggtcttcac aaaagacttc cagacgcatg ggaaccttca cctgaaaaaa
    10561 gaacaattaa tatgatgata tgacactatt tgaatagcag gaaggtttct gtcaacaatc
    10621 attgacacaa agacggaaaa acgggcactt cggcgtaaag atatccggtc agcacccgat
    10681 attgcattat ttttcgctgg gcgttttgcc tttttgcagc gtttctttca cctgaccgat
    10741 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
    10801 gcaaagctcc atagctttaa tccccagtcg tgccgtcaga agtcctgcac caatcccctg
    10861 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca ttcccacttc
    10921 gcgcaccaat tcgctggctc cggcgaaagc gatattcagc aataccagct tgaacaggcg
    10981 caaacggctg taatatccca gttcaatgcc atacagcgtg gcgatgcgat taatcaaacg
    11041 cagattgcgc caggcgataa atgccatatc caccagcgcc agcgggctga ccgcaatcat
    11101 caacgttgat tcagctgctg agcggctgat ttcgcgccgc gcctgggcat ctaaaaccgg
    11161 ctggaccaga tgagcataca ggctgaccac ttcacgatcg ttctgcgttt catggattga
    11221 ggcataccag cgttgcagcg ctggatgaga ctgatcaata cccgcctgct gcgccagttt
    11281 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctgtgc aacaaatcgc gcgcttcgtc
    11341 gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc cactctgtta ccacagaacc
    11401 tacgccagcg ccgataatca atgccccagc agcacatcca cccagcgcca cccagtcctg
    11461 agtttgccag gcattcattg tccactgtac accctgcccg acaacgcttg cgccaaacag
    11521 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcgtc
    11581 cattaccgct tcgacttgcc cctcttcttc ctgcgcttcg tcgagcgtgg ccggggcaaa
    11641 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgttcgac
    11701 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
    11761 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
    11821 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tatcccagaa cgcctgacca
    11881 ggcaaacgtg cgggaacttc gccaggataa acagtgagcg gtgcgccatc gctaagtcga
    11941 ttaccgcgca gcgccgggat tttctcaccg ttaacatcaa taatgccact ggtggtcgcc
    12001 tgaactgacg ccagccccag gcaatccatg ctgatccctt cgaacgccgc attttgccag
    12061 gcatcctgaa ttagttgttg cagcaatgaa accatattgg cgtgctgatc gatggtcaca
    12121 tggtccgctt tagtggcagc aaacaatagc ttatcgataa ccggcgaaaa caaacgcctg
    12181 aacagggtac gctgcccata gtggaaactt tgcatcagct gcgtcagcgc cagacgcata
    12241 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
    12301 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
    12361 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
    12421 tcgccccagg catcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
    12481 tcccccggca agacaaagcg cccaggctga ataaagtgca gcccctgctg tttacagtgg
    12541 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggcgct
    12601 agcgggtcca gcccttcgca catcattcgc catttcgccg accattcgcc gcgctgacca
    12661 ttgagtaagc ccgtcatctg gcgcgaccag cttaaatagt cctgcgccag catcggcagg
    12721 tcgagcaacc attcgccagg gtaatccaca atctccagat acagcgtgga ggtatcctta
    12781 aagtggcgca gcagcgaatc gttcgattta tagcgtagtg ccaggcgaat ttcgctgacg
    12841 ccgcgcgttg gcgtcggcca ggcgggagga tcgccataca gctgcgccaa cccttcgtcg
    12901 taggtaaaac gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcagacgc
    12961 tcttcacgca ccgcacttaa tagcggcaaa cgtgctccgg catgaatatt gagcaactga
    13021 ttgaccatcg cagtgataaa cgccgttttg ccgctgcggc taagtccggt tacggcgagg
    13081 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
    13141 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcataagga tatgaatgtg
    13201 ggggccaggc cattgattat caactacccg tgttgtcatc gacgggcgaa ctccataaga
    13261 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
    13321 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaaccaat
    13381 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcaacca
    13441 aacaagctgg cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
    13501 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
    13561 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacac cccggcagaa
    13621 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
    13681 agttcgccag cggatgagtc attttttgcc caacttgata aatacgatat tcctgtggtg
    13741 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
    13801 ggtgacagca ttgcgttgac cgatgcgtta attgaaagtg gacatcaaaa tattgcctgt
    13861 ctgcatgcac cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagt
    13921 ctggaagatc ataatattgc agtgcgtgat gaatggattg ttgatggcgg ttatacccat
    13981 gaaacagcac tgcaagcagc acggcaatta ttaagtcagt caccgttgcc tgaggcagtg
    14041 tttgccactg acagcctgaa attaatgagc atttatcgtg cggcggcaga gaaaaacatt
    14101 gctattccgc agcagttagc ggtggtgggt tatagcaatg aaacgctgtc atttatttta
    14161 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcgaca aagctgtgag
    14221 ttattattcc gcttaattgc cggaaaaccg tcaccacaaa atattaccgt tgccacgcat
    14281 atgacgctga aataaatacc agcgaccttg cctgaatgcc ggatgcgatg ctggcgcatc
    14341 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
    14401 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc gctgtcgcct
    14461 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa tggaaagacc attctggaaa
    14521 tcataaccgt aaaataaacc aacacggtta aaatcatggc cttcacgttc aatatcatcc
    14581 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
    14641 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttatta
    14701 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
    14761 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggtc
    14821 gtgttcaaat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
    14881 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
    14941 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
    15001 aaatcatcgc tttcgaggaa ctgataatgt acctccagct ccgggcgatc aaaccacgtt
    15061 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agaaattctc
    15121 cacggcccgt tggcggcatt aaaatagaca gaaggctccg ccagcccatc catatcttcg
    15181 ccataaccct cgacgttttc tatttcgtac atcgcgccga tattaaagtg ccagtcgttc
    15241 ttttcctcgg cctgtgcgca ggccattccc gcacacatca ccagtgcggt acagggtaat
    15301 aactttttca ttattgttcc ttgtattatc ctgggggaat gccttccccc tgtatttagc
    15361 gaattgctat ttccgtttct gcatcaaaga aatgacattt agtcatatca aaatgaatag
    15421 tgatattttc tcctgcatga taatcattta acgcaccagc acggaccact aactcgtgcc
    15481 ccccaaccgt ggtgtagagc ataaattccg caccggttaa ttctaccacg ctaattttgg
    15541 cggaaatatt attttcctct tgcgcgtccg gatgaatatc ttccgggcgc attcccatca
    15601 cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc taatttctct tcgggaatgg
    15661 ttaatttaag cgtttccgta acgaatttat cgccatcgat cgtgccgcga ataaaattca
    15721 tcgctggtga tccaataaat ccggcaacaa acatattcgc gggttggtta taaacggttt
    15781 tcggcgcacc cacttgctga acaatcccgt ctttcataat cacaatccgc gtcgccatgg
    15841 tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt ggtgttcagt ttctgatgca
    15901 gcttgctgat ctctgcgcgc atttgtacgc gcagcttggc atcaaggtta gagagcggtt
    15961 catccattaa aaacacgccc gcttcgcgca caatcgcccg cccgagcgcc acacgctggc
    16021 gttgcccgcc ggaaagcgcg cccggcttgc gcttcaggta ctcacgcagg ccgagaattt
    16081 gcgccgccca gttcacccgc tcatcaatca cctctttggc gattttttgc atcttcaggc
    16141 caaacgccat gttgtcgtaa accgtcatat gcggatacaa cgcgtagttc tggaacacca
    16201 ttgcgatatt gcgtgctttg gctggaacgt cattcattcg tttgccgtcg atcaacagat
    16261 cgccgccgct gatctcctca agcccggcaa tcatgcgcag ggtggtcgat ttaccgcagc
    16321 ccgacgggcc gacaaatacg atgaactctt tatcggcaat ttccaggttg aagtccttca
    16381 ccacatgcac ctggttatcg tagatttttt gaatatgttg taacgaaagc tgagccatgt
    16441 tgattccttt gctatacgtt ttgccagaag gccgataacc gcggccaggt cagggagtcc
    16501 gttgagggca acaataattg cgccccggtt aagcctgcgc cgatccccac cgagcgcata
    16561 ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt caatgccgat acatgcctgc
    16621 ggcggcacgc ccagcccggc acaggcagcg agaaagattt ccggatccgg tttcgagttt
    16681 ttaagttggg aagcatccgc gcagaaggtg aaaaactcgc gcagctccag cgccgctaaa
    16741 atcgtcggcg cattcaggga gacagaagcc agccctaccg ggatctgctg tgcgcggagc
    16801 tctgccagca aattgcgaat accgggcagc acagcattaa ccgtcagctc gcgcagtgag
    16861 tggacgtaaa gcagattttt gcgatacgcc agttgcgtcc gctcctgcgg gttaaagtcg
    16921 ccctctttgc ccccgtgttg cagaatgcgc cgcagagact catcgcggct gatccctttt
    16981 agggattcgt taaactgcgc atcaatgctg atgccaattt cagcggcaat ctgctgccac
    17041 gcctggaaat gcagatgcgc ggtatcggtg attacaccat ccagatcgaa aattacccct
    17101 tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc attgaagggc aaaataaaat
    17161 cacccaaaca gaaaacagat tcttcagcca cggatataag ctgcccgttc aaacgcagtg
    17221 aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc gagcgtgacc tgtaattcgc
    17281 agccctgcca gaacagaggg aaagacaact gttgccactg ctccggtaac gccggattga
    17341 gatgtaattc accgtcacgc acgctcaccc cggcaaaacc ctgaatcgcc cccagccaga
    17401 tagcgccagt tgcggcagcg tggataccat catcacaact atgcggatca gcaccaagat
    17461 cgatttcagt cccctcgcgc cagaactgat aactttgggt cagcaggcca cagcgtgcgg
    17521 caacaatgcc gtggattgct ttacttaatg acgagtcgtg aatagtgcgc ggttcataaa
    17581 attgcagatt ggcaagacac gataccgctg agaactgctc cggcagcatg taattgagca
    17641 tcaccacatc agcttgtttg aggatctgca tctcgttcac ttctgcgcgt gaataatcca
    17701 gcagaatggt ttgcttcccc gccgccgctt tgtatttagc cagattaatc gccggcttag
    17761 ccataaacga atcatcctgc ggcaaaacgc catcgggctg cgtttctggc atccatagct
    17821 ctttgaggaa catttcggcg cgatggataa acgcatcgtc gctacagccg aactggcggg
    17881 caatattcag cgcctgttga acgttgtagc gggccatata gctggtgaag gcgttattat
    17941 tgacatgttc ggtatattcg tctggcccaa taacatcatg aatttccaga cggtcgttaa
    18001 ccctcaccgc gcggctaatc cagaactttg cagtttccag aagtagcgcc atgccttcat
    18061 gagcaatgaa actttcatcc cccgtggtct gccagtattg aataaccgcc caggcgatat
    18121 cggccaccag atgatgttcc gcctgcgccg aggccacttt ttgccgcagc ccggtacgaa
    18181 tgttaatggc ggcaaattct ggcgtctctt cttcgccgct gcgcgcgctt tcccacggaa
    18241 atagcgcgcc ctgccagccg ctgcgccgtg ctttctcctg cgcgcctggc aagttgtgcc
    18301 agcgataacg cagtaaactt cgggcaaccg tcggatcgct aaacagatgg aacggcagca
    18361 aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc ttcgccggtt aagccttttg
    18421 ccgcaatact gctgcgctcg tcgtgagccg gcgtcatgat gcgcagatga taaagcgcat
    18481 aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac cgtgatacga cgtttctgcc
    18541 accattgacg ccagttttct gttgatgctg caagaagttg gtcgtaactc tgctgcgcgc
    18601 atatttcaag ctggcgaagc gacgcgctgc cccactcgtc taaaacggct tgcctgtcat
    18661 cccgccagtc gatccacacc agtttttgca acgtcactgt ctcgcctgca tgaagctgcg
    18721 cactggtatg ttgcagcaaa cggcgctctt tggcggtata gcattgctgc acatcaccgc
    18781 tcaccttaca gcaacagctg atggccacat cactgcgtcc atcctgggtg gtgtagatcc
    18841 cctgcatcag atgctgacca aacacccgca cctgggtttc gtcgagatgt tggcgaccgt
    18901 ggttggtttg cgtggcgtcg atacctgttg aaatcagcac tgacgcgtcg gcgtccagtg
    18961 gcgtaatagt gatttccagc gcaatgagcg gcagttggtc tgccgaaaca aagcgacggc
    19021 tggcgatggt gtaacctgcg ccgttgctgg tacgccagac aacgttgcgg cgtaattcgc
    19081 cactggcaaa gtcaagctca cgctgccagg cttcgtggga taacgagaaa acctcaccat
    19141 ttatggcaat ctccatcccc aagatatcag gcaggttcac cagttcgttg atttcacctt
    19201 ttcccgcccg atgatacagc cccgccagat acatccctcg cgtctggcgc gtgtaatctt
    19261 cttcatggct ggcgcgaagc ccaagatagc cgttcccctg cgccatcagc gatgcatact
    19321 tgttcagggt atgctggctg aaatggggtt ctgataacgt tactggcctg gtcataattc
    19381 cacacgcgtc cctgtttctg cggattgata taacgccgcc accagttgct ggatgatgta
    19441 cccctgctcg gcgtcggcta tcatcacggg cttgccctgt acgtgattga taaaggcttc
    19501 catgctgccc aaatggcggt tgtcgtctgc catttcccgt tgcatcagcg tcattaattc
    19561 accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc gcaccggctt tatcaccaca
    19621 aaagctgacg ttcataatcg actgttcgcg gatgttgagc gcaaatgacg tttccagcca
    19681 cagaatgccg ccgttatgaa attcaatggt gccaaacagc gaatcttcga cgctgtaagt
    19741 tgccggatcc cactcgccaa attgaccaca gctcttttgc gtgccgatct tttgaaagct
    19801 atgcgcattc acgcttttca ccgcaggata gcccagcaca tacatcgcag catccagcat
    19861 atgaatgccg atgtcgatca gcgggccacc accctgcagt tctttattgg taaagacacc
    19921 ccagccggga acgccgcagc gacgcagggc gcgggcggtg gtaacgtaaa tctctcccaa
    19981 aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg agcgcaaaac gatggtgaaa
    20041 gtcgtaggcc agcaccttgc cctgtttgcg cgcggtatcg cacatttccc gcgcctgttc
    20101 tggcgtcatg gcgggcggtt tttcgcacat cacatggcag cccgcttcca gcgccatcag
    20161 ggtatgttcg taatgaaaac ggttaggtga gcagacgcta accacatcag gtttcaccgc
    20221 cagcagcatg gcctgcggat cgtcccacac ggatgcattc ccgtattttt ctgccagcgc
    20281 ctgcgcctgg gaaaggcggc tgtcacagac agccaccagt tccagatcgt tgcgggtgca
    20341 gtagtacgaa gcatgaacct tatccgccac ctggcctgcg cctattatcg cgacccgcag
    20401 tggagagctt gtcattgcac ttttcacaaa gacctcttag caggtacgca accaggtcaa
    20461 cgaatcacgg tacgcctggg caggatcttc tgcccggata cgcccttcat acaccacata
    20521 accctgatag ttatcggcgc gcagctgttc aaacagcgcg tggaaatcga gggtgccgct
    20581 gcccggctgg taacgatggt tatcggcaat atgcacatga ccgagcaagt cgcggttgtc
    20641 atgcagcgcc tgcgccaggt tatcttcttc gatgttcatg tgatagaaat cgccgataat
    20701 ctgcacatgt ttaagattgt tttcgacgct gtaacgacga gcatcggcga gggtgttgat
    20761 catatgatcc tgatagcggt ttaacggttc gagatacacc acggttccgg tacgcgcggc
    20821 gacctgttcc agtacgcgca gggaatcact caccattttg cggtcgccgt ccaggctacg
    20881 cggcgaggtc atcggcggta agcggaaggt aaacatgccc cacgctgccg gaacgacgat
    20941 acctttaccc cccacttctg ccagcgcttc gagaatgcgt tcgatctgct ttaagccatt
    21001 aagacgacgc tcttcgataa agtcaccaat ccagccgtca tagccgccgc aggcggtggt
    21061 caccggtaaa ccggtttctt tgatcgccga tttgacttct tcgatgttgt tgaccagcag
    21121 tttgccgtca atctcaaaac catcgaagcc catctctttg atataacgaa atttctccag
    21181 aatattttcc gggaagaacg cctgattctg tgtgcctatt ttcatttttt cattcctttg
    21241 aattaaaacg tgacgcccat tttgatgctc tgttccggat gctgatcgac atactgcatg
    21301 tagctttccg ggctgttggc aaaggtcact accgggtcga ttaaatcttc gcaattgaga
    21361 taaccgttca tcagcagttt ccagcaggtt tcttcaatac gcttacggct ccagcgcgga
    21421 taatccgggt tcggttcact gcacgcgcga gagaagacaa ttttggcgtt attgaaatgc
    21481 gcttcgcgtc cgaggttaaa accttcagca aacggtttag caaacgcgac ataggagatg
    21541 gtgccaccgt aagccaggcc gcgcagcgcc gactgcagcg cgtcggcgta accgctggtt
    21601 tcgataatca catcggcacc ctgcttgccg gtcagcgttt tgatctcttt gcctacgtca
    21661 gtgccaatgg gattaaggca gaaatccgca ccgtgacgac gagcaatatc acaacgatgg
    21721 gcaataggat cgacgccaat taccaccgac gcgccagcgc gtttagccag ttggatggca
    21781 atttgaccga tcgcgccaag ccctaccacc actacaaaat cccctacgcg tacgttggca
    21841 tcgcgaacgc cactcatggc aaactgcgcc gggtcgtagc agacagcgtt tttccaggag
    21901 ctgccttccg gcattttgcg cagcttgtag ttattcactg cgttaatgat gatcgtctcg
    21961 gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt agtcggtaac gtcgctgccg
    22021 cactcgataa tgtcgccaac caccatgttg ccaagctgga atttgccaaa ctcaatgccg
    22081 cgcggcgcat ctgccggacg cggggtgaac atctgccatt cgccgttaaa gtcttcatcg
    22141 ataaacgggc tggcggcgcg gaagtcgacc acttccgttc cgtgtttcgg tgcaccgaaa
    22201 cgcacgcgga tcttcacttc attggctaaa atcgcccgat cttcatactc aaccagcgca
    22261 gcaacgcgcg gtgctgtggc tactaacttt ttcatgtaaa actccttgta aagaaactgg
    22321 ccgctttagc ccttcacgcc accggcggtc aaaccactct taataaaacg ttccgaaagc
    22381 gcatacataa tcaccaccgg caatgcggtc accagcgagg ccgccatcat ccgtccccag
    22441 atgtaatctg gcgtactgaa cagcgcgttc aggccgactg gtaaggtgaa attgctggcg
    22501 ctggagagga aaatcgacgc aaacagatag tcgttccatg ccaccataaa gcagtagacg
    22561 aatacggata tcagcccgga catcgccagg ggcacggtga tacggaagat aatttgcaga
    22621 cggttgaggc catccatcat cgccgcttct tcgatctcat cagggatggt gtcgaagtaa
    22681 cttttcagca taaacacggc ggtgggcaga gtttgcgtca ccatggtaat gatcagcgcc
    22741 atctcggtgt cgtaaatgcc aagtgcggtg ataattttga acagcggcac caccagcaaa
    22801 atgccggaga acatatacac cgtgtaaaag ctggcgttga tggtcatccg gcctttaaag
    22861 cgcaggcgag aaagcgcgta agctccgaga atgccgagaa ataccgccac cacggatgaa
    22921 accaccgaca ccaccatgct gttacggaag tagtcgacaa acgggaaaat caccgggtta
    22981 aaaatgtcga cataatgctc cagcgtccac tgttgcggca gcagcgtagg atgcagcgag
    23041 atcgcctctt ttgcgctctt gaacgaggtc atcagcatca caaaaaacgg aaacagcgtg
    23101 atgatgagaa acagcgccag cccgcggtaa aaaccgatac gactgagtgt gcgtttattt
    23161 gttgccattg aggttcaccc gtttcctggt cagcagaatg acagcgaaaa taatgatgaa
    23221 gagcaccacc gagatcgccg ccgctttccc taagtcatta aaggcaaagg cggttttgta
    23281 gagatagaca ccaagaatat cgaccttggt tgtcagcaga taaacatccg cgaacatgta
    23341 gaacatccag atggtgcgaa gggtcaccac cgtcgccagg accggcataa tcgcgggcag
    23401 cgtgacgata cgaaaccgtt gccaggcatt agcgccatcc atctctgccg cttcgtacag
    23461 cgatttgtcg atggtctgca aaatcgccag aaacgagata aaggcatacg ggaagtagcg
    23521 ccagatggcg aagatcacca ccagcacaaa actactgccc ggattatcga accacagcgg
    23581 tgcctgctca taaagatgca gaagatcgac gccgaggtag ttcacaatgc cgtagccgtt
    23641 gttgaacatg tatttccagg cgaacaccag cgaaatagac ggcgttacgt aggagaggat
    23701 caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg ttgaaaaaca tcgccactgc
    23761 cagcccgagc acggtgctgc ccgccaccac caacgcggta taccagaccg tcatccacag
    23821 cgagtgccag aagccgggat cggagagaat acgcacatag ttgcttaccc caacaaacgt
    23881 tgactcgatg ttgggattga gcggcagacg taaaaaacta atttcgatat tcgacaccat
    23941 tggccacgca accagaccgc ccagcagtaa taagctgggt gcgagaagca acagcgcaaa
    24001 gggcatatcg gaacgacctg aaaaaagcct gttcattgcg gattcctgtc ctgggtttag
    24061 tgctgttcga tcagttcatc cagctttttc tggctcgttt gcagcgtgcc agggagatcg
    24121 gctttaccta cggtgacgtt atgcaccatg gagctcacca ccccagaccc cgtcacatcg
    24181 cccatgcggg taaatttttt atcccctact gcgccaaaga cctgaatatt cggcagttca
    24241 ccgattaact gatttggcag ttcacccagc gccttaataa cgtcgttgtc tttccaggtg
    24301 gaagtagtga caaccgcttt gttcaccggc agcgccgcgc ctggcgacat catcacccag
    24361 tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt tttcagccgc ttccgtttct
    24421 tcagtctttt gtccggcggt aatggtcagc gaggtcaaca tgccgtagac ggcagagttt
    24481 ttctcggttg gcaccacgaa accgacgttt ttcggatcgc cttctttaat cacagccgga
    24541 aggatatagg tggagtaaat cgccatcggc gcggtgccgt tcataaaggc gtctttcact
    24601 tccatgatgt cgttagaacc cggcatggtg ttggcagcaa ggtcgcggta ataggtcagc
    24661 gcctgcatca tctctggtga atcaagggtg attttgcctt cggcgttaaa gacgttggcc
    24721 tagttggata acgcaaactg ggagaaggat tgttccgtca gcacgctttc tgccgtaggc
    24781 agcgcaatac cgtatttttt attcgccggg tcattcagtt tctgtgcaac gtccagcagc
    24841 tgttgccagt ttttcggctc ctccagcccc gcttttgcca gcacatcttt gcgataccag
    24901 atgccgccaa tccaggcgct gacaggaaca ccggtccatg cgctaccatc ttcggtacgc
    24961 acaatacgca gtacgccatc gtaaaacgcg ccttcaccaa cgttgctgat gaccgtggca
    25021 accgccttgc gatcgataag ctgctctttg tccataactt tggcgtagtc atgactggtt
    25081 tcaatcactt ccggcagcga gccgctacgt gaaagagtga tgactttggt gttataagcg
    25141 tcctcttcga cgggcacttg tttaacggtg atacctgggt tttctttttc aaaacgggca
    25201 atcaatttgc tgataactgc ctgacgctcc tgctccaccg aagaatgcat aaattcgatg
    25261 gatacatggg gtttattttc ttctttacag cctgaaatca gggcgcagga aaccagtgct
    25321 gataacagca cgatttttga tttaatcatg agatgttcct ttttatttaa tccacataac
    25381 ctgccagggg cgcagcgtta ttttattacc caatatatca acttcactaa tcagatcccg
    25441 accgtgcagg ccagtaatat ttacatgctg aatattttta ctgacattaa acaatccggt
    25501 cagacaatta ccctctgcgt tacttctttg aatacgcatt acggatgaat taaccgtatc
    25561 gatggtaaaa ttattatccg gatgaaactc gttatggctg cgacgaagtg taattaaacg
    25621 cgacaactca tgatataccg catgccttaa tgtagcttca tcgttcagtt ctcgggttat
    25681 ctcttcactg tgatattttt tacggttaat cgcacggtta tatccgagtt tttcgacacc
    25741 tgcgtaatca ttacgcgagc ccagaatact ttgaatatat atcgctggca caccggggaa
    25801 acttaacaaa atcgcatggg caaggataaa cctggcgcaa cgttcttcat cgctactctc
    25861 gcggcggctt aacgcatcca tataggtgac atttatttca tagggactgc gcgtaccgtc
    25921 gggattattt ttccagttta ctaatgcacc ttcctgctgt aatgcctcga ccagcgctaa
    25981 tatttcgctt tctggtaaca agccccgcag tgggttcagc ccgatgccat cgtgagaggc
    26041 gaggaagtta aaccaggtcg ttttaccgga gggcaagctc agactttgcg cccactggca
    26101 cagcgcctcg acgttctgtt tttgcaccgc atgtaacacc agcggcggca gcgagaactg
    26161 gtacaccata tgcgcttcgt catcgccttc gccaaagtaa gcaatgttat ctttatgcgg
    26221 aacattcgtc tcagtaataa tcaccgtacc tggcgcgatg tcatcaataa tcgaccgtaa
    26281 cagtttgata atcagatgtg ttttttccag atggatgcag cttgttcccg gctctttcca
    26341 cataaagcca acggcatcca gacggacata ttctgcgccc ttttccaggt aacaaagcaa
    26401 aacatccacc atcgccagca acacgtcagg gctacggtaa ttcaggtcaa tttgatcgtc
    26461 gctaaaggtg gtccataaat ggcgcgtgga attatcatcc atttgaaacg gcgttaatag
    26521 cggtaatgca cgcggacggg tgacagcgct gagatcggtt tgcggatcaa ccgcaataaa
    26581 aaaatcttca aaacctggct gctgttgtaa atagttttta aaccattcac tttttgccga
    26641 catatggttg cagacaaaat caaacattaa atgactgcat tcaccgagct gctgaatatc
    26701 ctgccactac ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa tccatcatca
    26761 gatgaccatg gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga
    26821 tggtaaaact gattaaatgt gggtagtggt tttaaatcat tactgtgaaa ttgatcggca
    26881 taggtaatta acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat
    26941 cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca
    27001 ccgtagattt cgtccaggta atccgtaatt ttctgtttca tgttgccttt cctggtagcg
    27061 gtcccatgaa atgagagact actccgcaga aaataaactg tcaattgagc acaagggttt
    27121 tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat
    27181 aatctatagg gatgtaaata aggtgtgaca gaaaaaaacg gcgcataagc gccgctcatg
    27241 gtgaattctt ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct
    27301 caacgtgggt atatcccatc tcgctaagga tctcttttgc ctgccctgat tggcgtccgg
    27361 cattgcaata cactttcacg gtgtcgtttt tatccggaac gacggtggca atgcgctctt
    27421 tcacttcctt caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg
    27481 gaacacgaac atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca
    27541 gagcaagtaa gcctttttta aacatgtaag cgtccttcta tggatgaata ttgctatcca
    27601 ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat aacgctgcgc
    27661 cagtttattg gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag
    27721 cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgctgggc catagcgcag
    27781 tgcggtaaga agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc
    27841 ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg
    27901 aatgtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt
    27961 tcacggtcga cttcatccag caattcactg ctggaaggta gctgctcacc aaaggccatg
    28021 ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc
    28081 gccagaccga agaaaatcga caacaccacc aggatacgca ccagttttac cggtacatca
    28141 aaatagttgg caatcccggc gcagacgcca cggaccatgc cctgctgtgg aatacgccat
    28201 aattttttat tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat
    28261 caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt
    28321 gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga ttgctgtaat
    28381 gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaatgga atagccagaa
    28441 atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg
    28501 atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gctcgctgat
    28561 tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct
    28621 gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt cgaaacgagc
    28681 cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc
    28741 cgcctgatgg cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc
    28801 cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg
    28861 ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgccg cacgcgccaa
    28921 atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc
    28981 cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccagcgcac gcgccgaagt
    29041 agaacgtact tcaaccaatg tatcttccat ctcctggatc atcagacgca ccagtttctg
    29101 tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg
    29161 agaaaaaata cccataatgt tgtcctcttg atttctgcat tcaggattgt cctgctgaac
    29221 tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag
    29281 aatcaacagc aacatgccag gatgaattag ctaattacac taacaagtgg cgaatttcat
    29341 catggcagaa tacaaagata atttactcgg tgaggcgaac agctttctcg aagtgctgga
    29401 acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac
    29461 cggtaaagag ctgatcgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt
    29521 tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca
    29581 cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga
    29641 tggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt
    29701 attgcgcgtg attgagtacg gtgaactgga gcgcgttggc ggcagccagc cgttgcaggt
    29761 gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggtac
    29821 ttttcgcgct gacctgctcg accggctggc tttcgatgta gtgcaactgc cgccactacg
    29881 cgaacgtgaa agcgacatta tgttaatggc ggaacacttt gccatccaga tgtgtcggga
    29941 aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta
    30001 tcgttggcca ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag tgtatcgcca
    30061 cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc
    30121 tgaagacgct atcgccgttt cagaaaccac ctcgcttcca acactgccgc tggatttacg
    30181 tgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac aagggaaata
    30241 taaccagaag cgcgcagctg aattgctggg cttaacctat catcagttcc gcgcgttgtt
    30301 gaaaaagcac cagatttagc gaagttgggc aatacaactt gccggatgcg gcgtgaacgc
    30361 cttatccggc atacataatc aatacgtcgt ttgcaggtct gataagcgca gcatatcagg
    30421 cattattgcg ttgcccctgc ttctgcgtat taatctctca gcgcatcctg caaaattttc
    30481 attgccgcat cgaattgcgc atccgggatg gtcagcggat acaggaaacg aatcacgttg
    30541 ccgtatgcgc cacaggtcag tagaagcagt ccctgcgcca gcgcgcgttg ctgaattttt
    30601 tgcgcaatgg cggctgacgg ctcgcccgtt tgcgggtcgt taaattctgc cgcaatcatc
    30661 gagcccagac cacgtaccgc cgcgatggcc ggaacgtttt ctttggcatc aatcagggtt
    30721 ttcgtcagac gctgtcccaa ctgcatggcg cgctcacaga gtgactcttt gtcgataatg
    30781 ttgagcaccg cgtgcgcggc agccaccgcc agcggattcc cggcgtaggt gccgccaagc
    30841 ccgcccggcg cgggcgcgtc cataatattc gcgttaccga ccacacccga aagcggcatc
    30901 ccgcccgcga ggcttttcgc catcgtcatt aagtccggct tatcggcgta atgatccatc
    30961 gcaaacagtt tgccggtacg cgcaaagccg ctttgcactt catcggcaat catcacaata
    31021 ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct cttttggcgc aacgttgaaa
    31081 ccgccctcgc cctgcaccgg ttcgaaaata atggccgcga cctgcttcgc ttcgatgtct
    31141 gatttaaaca agcgttcgat ggcgtcgagg gagtcctgtg ttgaaatgcc gtgtaaatct
    31201 gacggataag gtacgtgata caccgaacca gggaacgggc cgaagccgat tttgtacggc
    31261 gcgacctttc cagttaacgc catagtcata tacgtacgac cgtggaagcc gccgctaaac
    31321 gcaatcacgc cagggcgtcc ggtatgggcg cgggcgattt tcaccgcgtt ttccaccgct
    31381 tccgcaccgg tggtgaagaa cgcggttttg gcctgcccgc tcaccggggc aagggcgttg
    31441 attttctccg ccagggtgac gtagctttca tatggcacaa tctgatacgc ggtgtgggta
    31501 aactgttgca gctgctgctc caccgccgcg accagatcag ggtggcgatg tccggtattc
    31561 agcaccgcaa tgcctgcggc gaaatcgatg tactcgttgc cctcaacatc cttcagcgtg
    31621 gcgttttcag ccgactgggc gaagaagtta cacatcacgc caaccccgcg cggggtggca
    31681 gaaagacgac gctgatggaa ttcattgttg ctcatgtttc acaccttttg actcttcact
    31741 gttaatcgga aattaaacgc ccagacgatc gcgcaggctg taatacgccg cgcccatcgc
    31801 ggtaaacggt acgcgcagcg tgcgcccgcc ggggaacggg taatgcggca gattggcgaa
    31861 ggcatcgaaa cgttcggcgt cgccgcgcag cagttcggca atcaaacgtc cggcgagatg
    31921 agtacaggtg acgccgtggc cgctgtaacc ctgcatgtaa tagatgttgg tatcgaggcg
    31981 accgaactgc ggcatacgcg acagggtcag caggaagttg cccgtccagc gatagtcgat
    32041 tttcacgccc ttcaactgcg ggaaggtttt cagcagtttc ggcaccacaa gacgctcaac
    32101 gtcatccggg tcgcgcgcgc cgtacaccac gccgccgccg tacagcaaac ggttgtcggc
    32161 ggtaagacgg tagtaatcaa gcagataatt acagtcttcc acgccgtagt ttttcgggat
    32221 cagcgaacgg gctaaatcat cggaaagccg ctcggtggtg atcacctgcg taccgcacgg
    32281 catgctgcgt ttcgccagtt ccggctccac tttatcgccc aggtacgcat tcccggcgac
    32341 aatcacatac ttcgccgtca cctgacctgt ggctgtacgc actacggcgg gcgtggtgtg
    32401 ctggatctgc gtcactgcgg aaagctcata cacgcgcccg ccgttcaggc ggatagcgtc
    32461 cgcttcgcca atggcgaggt tcagcgggtg aatatgcccg ccgctgtgat ccagcagcgc
    32521 accagtgtag cgatcgctgg cgacctcgcg gcgaatggcg ttggcgtcca gcatctccag
    32581 ctgtttattg ccgtagcgtt cccagttctc tttctgctct tcaagtgtgg cgagctgttt
    32641 atcattcatc gccacaaaca gaccgccggg gcggtagtcg cagtcaatct gataacgttt
    32701 gatacgttcg cggatgatct caccaccttc gaacatcatg ctgccgagca tacgggcggt
    32761 gtccatgccg tagctttttt cgataacgtc gatgtcgcgg ctgtaggagt tcacaagctg
    32821 cccgccgttg cgcccgctgg cgccaaagcc gatgcgtgag gcttccagaa ccaccacgtc
    32881 aaagcccgct tccgccagat gcagtgcgga agacagcccg gtatagccgc cgccaaccac
    32941 gcaaacgtcg caggaaattg actcattcag cgtgtcgaat ggcgcgtatt tattcgcact
    33001 ggcggcgtag taactgctgg tatgttctgt catttcaggc ctccaggctt atccagatgg
    33061 ttttcagttc ggtgaatttt tcaagggcat gcagggattt gtcgcgaccg ttgccgctct
    33121 gcttatagcc gccaaacggc acggtcatat cgccgtcgtt gtagttattg acgaagacgg
    33181 aaccggcttt caggcgtcgg ctcatgcggt gcgcgcggga gaggtcgcgc gtccataccg
    33241 ccgcgccaag gccgtactgg ctgtcgttgg caagctgtag cgcctgttct tctgatgtga
    33301 aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg gcttaaggac gcattcgggt
    33361 ctacatccac aatgatggtc gggccgatgg cgacagccag cccggcgtta cggccatcca
    33421 acaacagttg ccctttgctt tcgccttcct gaataaagct atggaccgag tcggcgtggg
    33481 cgcagtcgat taaggtgccc atggtggttg cgggatcaag tggatggccc ggctgccagt
    33541 tttgcgcctg ctgttttaac agggctaaga attcatcggc tatgctctct tccagcaaca
    33601 ggcgcgttcc ggcgatgcac acctgcccct ggttgtagaa gatgcctgct gcggtggcgc
    33661 ttgccgcctg ttgcaaatcc gggcagtcag cgaaaacgat gttggcgctt ttgccgcccg
    33721 cttccagcca gacgcgtttc atgttgctgt cgcctgcatc tttcagcagc tgtttcccgg
    33781 tacgggtcga accggtaaag gcaatggcgt cgatatcgtt atgacgcgac agcgcctgcc
    33841 ccgcttcatg accaaaaccc gtcaccacgt tcaacacacc atccggcaag cctgcttctt
    33901 tcgccagccc cgcgagacga atcgcactga gcggtgattt ttcagacggt ttcaggacaa
    33961 cgctgttccc tgctgccagc gccgggccaa gcttccagca agtcagcagc agcgggaagt
    34021 tccacggcac gatggcggca atcacgccga ccggttcacg cacgatcatc gccagctcat
    34081 ggctactggt ggtcgccact tcgccataca ctttgtcgat cgcttcggcg taccagcgaa
    34141 tggcgcgcgc cgcgccgggt atatcatcac gcagactgtg acgaatcggt ttgccggtgt
    34201 cgagagtttc cagcagtgcc agctcttcgg cgttggcttc tattaaatcg gcgagtttat
    34261 tcagcaccgc tttacgtttt gccggagaag agagtgacca gtcgccgcgt tcaaatacgc
    34321 cgcgtgctgc gctcaccgca cggtcgatat cgacgctctt gccgcgcgca atattcgcca
    34381 gcggtgcctg ggtaaccgga tcaacggttt caaaggtttc attttccgcc gcagcagtat
    34441 attcaccgtt aataaataag cggttttcaa tggcgagact taacgcttta tcctgccagt
    34501 aagccagatg atgaaaattc attatgactc ctgtttcacg tctatcagat atatgcagtt
    34561 tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc gtttatttct ggcgacattc
    34621 tttatggatt gccgggcgca ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt
    34681 ggtgggcgta tgggcgctga taattcggca aatacctgcc gacgtattac tgaaactgtg
    34741 cgggatgccg gtattaatgg cataactttg ccccgcgacg aggtggtaat cctgaccatt
    34801 aatcgtcaga ataatttcac cttccagtac agtgcctatt tcctcaccct gatgcttaat
    34861 tctttcccca gtggttgtgc ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt
    34921 cgggttaccg ttatgaacca gcttcattga cacaccctga ctgcccatct caattaaatc
    34981 gtcctgatta atgacgacct gcggctcatc aggtttttcc ggctcggaaa agaattccga
    35041 gagtgacaga ccatacacct tcagcagctt ttgcagcgta ctgatggcag ggctgacttt
    35101 atcttgttct atcgtactga tagcactgtg agtcagcccg gagagttcgg cggcacgacg
    35161 ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt tttcctggcg ccagtccctc
    35221 atcactcata tttgcatttc cttaaactgt agtggtcaga gtcgctgttt ttcagcgata
    35281 tggtgctgac aagcggtgat aaagccctcg aacaatatac gcgaaagcgc gtactcgcta
    35341 ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag gatgattgat gacactaacc
    35401 gcctcaacta agccatccgg cgagcgagct tcaacacgca accgtgggct aacgaccttc
    35461 gccccttgtc catgcagaga gtttacccaa aagttgctac attcaggtaa caatgcagac
    35521 agcaatcccc cctcttcaac ctgaacttca tgcgacggtg catattgctg ttccaccggc
    35581 agttcgggat cttcccgatg ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac
    35641 gaaccaccgg ttgccaccac cagttcctgt aaaccccggc agatggcgaa aatggggatg
    35701 cgcctttcga gtgcggcatt aattaacgcc atgctcagaa gatcacgctc gggatcggcg
    35761 tcaggctcat cgccgttttc accatatagg tgcggctgca cattgctggg actaccagga
    35821 aggtaaatgc catcgagttt cggcaaaagt tgttcaagta acgacggttc cgccagcgca
    35881 tgtggcagcg caattggcaa gccgcctgcg tggatgatgg cattcaggta cttttcttgc
    35941 agagtctggg tcgcatgccc cttaagcctg ttcctgcaca ttacgacacc gataaccgga
    36001 ttgttcatta tattttccat tctcaatctc acaaagtgga ctaaattatc gccattactg
    36061 cggtttaaat gacctttatg ttcaatattt tttcaatcta gcagtgggtt ttcatttttg
    36121 caaactcaat ttaacatttg acaaacattt agtttgcata cagattcgaa tggtggtcat
    36181 tatattttac gctttgataa cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc
    36241 aatatcgttg aagtagagaa ctttgttcag cagtcagaag agaggcgggg tagcgccttt
    36301 acgcaggaag tgaaacgcta cctggagcgc tacccgaata cgcaatatgt tgatgtttta
    36361 cttaccgatt tgaatggttg cttccgtgga aaacgtattc ctgtttcaag cctgaagaag
    36421 ctcgagaaag ggtgttattt cccggcctcg gtatttgcaa tggatattct gggcaacgta
    36481 gtagaagaag caggtctggg tcaggaaatg ggcgagccgg atcgtacctg tgttcctgtt
    36541 ctcggttcct taaccccttc tgccgccgat ccagagttta tcggtcagat gctcctgacc
    36601 atggtcgatg aagatggcgc tccctttgac gttgagccgc ggaacgttct taaccgcctg
    36661 tggcagcagc tgcgccagcg cggattgttc ccggtcgtag cggtagagct ggagttctat
    36721 ttactggatc gccagcgcga cgctgaaggg tatctgcaac cgccctgcgc gcccggcact
    36781 gatgaccgca atacacaaag ccaggtttac tccgttgata acctcaacca cttcgccgac
    36841 gtgctcaatg atattgatga actggcgcag ttacagctga ttccggcaga tggcgcggtc
    36901 gctgaggcct cgccgggtca gtttgaaatc aacctttacc atactgataa cgtgctggaa
    36961 gcctgcgatg atgcgctggc actaaaacgc ctggtgcgtc tgatggcaga aaagcataag
    37021 atgcacgcca cttttatggc gaagccgtat gaagagcacg cgggcagcgg aatgcatatc
    37081 catatcagta tgcaaaataa tcgtggcgag aacgtgcttt ctgacacaga aggcgaagat
    37141 tcgccgctgc tgaaaaagat gctcgccggg atgattgacc tgatgccgtc atcaatggcg
    37201 ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc cggggatgta tgtaccgacg
    37261 caggcgtcgt ggggccataa taaccgtacc gtcgccctgc gtattccatg cggcgatcgt
    37321 cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg ccaacccata tctggtgatg
    37381 gcagcgattt ttgccggtat tttgcatggc cttgataacg agctgccgtt gcaggaagaa
    37441 gtcgaaggta acgggctgga acaggaaggc ttacccttcc cgattcgcca gagcgatgcc
    37501 ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt taggcgaacg gttttgccat
    37561 gtgtatcacg cctgtaaaaa tgatgagctg ttgcagtttg agcgcctcat cacagaaaca
    37621 gaaattgagt ggatgttgaa aaacgcatag taccccttgc ggggttgttt catcggcctg
    37681 agcggagcca ggcctgacat ttgctggtaa tccagacatt tcccggatgt cgcgttatgg
    37741 agcgcaggca gcaacgggct tccgttaacg attaagactg tgacgaggaa atgagatgat
    37801 gccgatgatt aaatccccgc atggcgcggg aggctgcgta tgcgcccctc ctgcgactga
    37861 ctggactccg ccccctttgc ttcccttgct aaacaggttt gatttcaggt cgacacgacc
    37921 gcaaacctta ttacgcaggg gaggcagcaa ttatggctat taattcacca ctgaatattg
    37981 ctgcgcaacc cggcaaaacc cgtctgcgaa aatcactgaa attgtggcag gttgtgatga
    38041 tgggtctggc ctatctcacg ccgatgactg tgttcgatac ctttggcatt gtatctggca
    38101 ttagcgacgg tcacgtccct gcgtcctatt tgctggcgct ggcgggcgtg ctgtttaccg
    38161 ctatcagcta cggcaaactg gttcgccagt ttccggaggc cggttcggcc tatacctacg
    38221 cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg ctggtcatca ctgctggatt
    38281 atctcttttt gccgatgatc aacgttttgt tggcgaaaat ctatctctcc gccctcttcc
    38341 cggaagtgcc gccgtgggtg tgggtggtaa ccttcgtcgc cattttaacc gccgcgaatc
    38401 tgaagagcgt caacctggtc gctaacttca ataccctgtt tgtactggtg caaatctcca
    38461 tcatggtggt gtttatcttc ctggtggttc agggactgca taaaggagaa ggcgttggca
    38521 ccgtctggtc acttcagccg tttatcagcg agaacgcgca cctgatcccg attattaccg
    38581 gggcgacgat tgtctgtttc tcgttcctcg gtttcgatgc ggtgaccaca ctttcggaag
    38641 agacaccaga cgccgcacgc gtgatcccga aagccatctt cctgacggcg gtctatggtg
    38701 gcgttatctt tatcgcggcg tcgttcttta tgcagctgtt cttccccgat atcagccgct
    38761 ttaaagaccc ggacgccgca ctgcctgaaa ttgcactcta cgtcggcggt aagctgttcc
    38821 agtcgatttt cctctgcacc acgtttgtga acacgttagc gtctggcctg gcctcacatg
    38881 ccagcgtgtc acgtctgctg tatgtgatgg ggcgcgacaa tgtgtttccg gagcgcgtgt
    38941 ttggctatgt gcacccaaaa tggcggactc cggcactgaa cgtcattatg gtcggaattg
    39001 tcgcgatgtc ggcattgttc tttgatttag tcaccgcgac agcattgatt aacttcggtg
    39061 ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa tcatttctgg cggcgtaaag
    39121 gaatgaataa aagctggaag gatcacttcc actatttgct gatgccgctg gttggcgcgc
    39181 tgacggtggg tgtgctgtgg gttaacctcg agtcaacgtc actgacactc ggtctggtat
    39241 gggcttcgtt aggcggcgca tatttgtggt atttgatccg ccgctatcgc aaagtgccgc
    39301 tgtacgaagg tgacagaacg ccagtgagcg aaacgtaata gtcggatgcg cgccgcatta
    39361 tcctgcctga gggtaatgcg ccgcgccatt aactataaaa agatgtatga cccggatcac
    39421 gagtgctatc atccgccctt atgcttagcg cattaacccc aggggcaaat aatgaaccac
    39481 gatatcccgc taaaatattt tgatattgcc gatgagtacg cgaccgaatg cgcagagcct
    39541 gtcgcagacg ctgaacgcac accgctggcc cactacttcc agttactgct cacccgtttg
    39601 atgaacaatg aagagatcag cgaagaagcc cagcatgaaa tggctgctga agcggggatt
    39661 aatcccgtac gcattgatga gatcgcagag tttttgaatc aatggggtaa tgagtaatta
    39721 tttaattgca gacgatcggt cagtatcgtt gtccggataa agcgttcacg ccgcatccgg
    39781 ctaacaatga cacgcactgc agcgtgcttt aatgggtttc tcacgatcgt accgctctgc
    39841 tcctcgctaa gttgttctgt gagttatagt tatagagaaa tcacgggtac gaatgttgaa
    39901 cagatggcga cgccatctgt tcaacgacca accacacggc cctcattacg acccctgaac
    39961 acaaacggta atggaaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    40021 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    40081 caggcaatcg ccgcgcctga atcccccccg catgctgata tccgcgacag cggttttgtc
    40141 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    40201 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    40261 cgcctgatgc cagaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    40321 ttccacctgc gtcgcgatgt cccgtttcaa aaaaccgcct ggtttactcc cactcgcaaa
    40381 atgaatgccg acgatgtggt gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg
    40441 cataacgtca acggcagcaa cttcccctac ttcgacagcc tgcaatttgc cgataacgtg
    40501 aaaagcgtcc gtaaactgga taatcatacc gttgagttcc gtctggctca gccggatgct
    40561 tcttttttgt ggcacctcgc aacccattat gcttcggtca tgtcggcaga atatgcccgg
    40621 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg cactggaccg
    40681 tatcagttgt cggaataccg cgccgggcaa tttattcgcc tacaacgtca tgatgacttc
    40741 tggcgcggta aaccgttaat gccgcaggtg gtggtggatt taggctccgg cggcaccgga
    40801 cgtctgtcga aactcctgac cggggaatgc gacgttctgg cctggcctgc tgccagccag
    40861 ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc tgcgtcctgg gatgaacgtc
    40921 gcctatctgg catttaacac cgccaaaccg ccgctaaata atcccgctgt ccgccatgcg
    40981 ctggcactgg cgattaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    41041 acggcggcct ctattttacc gcgcgcctcg tgggcctatg acaacgaggc taaaattact
    41101 gaatacaatc cggcgaaatc gcgcgaacag ttgaagttgt tggggctgga aaatttaacg
    41161 ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc ccagtccact gaaaactgcc
    41221 gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa
    41281 ggtcgctttc aggaggcgcg gttgatggat atgagccatg atctgacgtt atccggttgg
    41341 gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt tactgagctg cgcggcaatt
    41401 cattcacaga ccaacctcac ccactggtgc gatccgaaat tcgacagcgt gttgcgtaag
    41461 acgctctcct cgcagcagct ggcggcgcgt attgaagcct atgacgaagc gcagagtatt
    41521 ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    41581 tacgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    41641 cgcgagaaac aggatgaggt gaaaaaacca tgattatctt caccttacgc cgcattttgt
    41701 tattgattgt caccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcactc
    41761 cacacgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc
    41821 tgatccactg ggattttggt gtttccagca ttaacggtca accaattgcc gaacagttaa
    41881 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    41941 ttggcatccc cgtggggatg attgccggga ttacgcgcca taagtggcag gataacttga
    42001 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgttga
    42061 cactgttttg ttcactcacg ctaggttggt tgcccgtttc agggcgtttc gatctgctct
    42121 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccat
    42181 ggcgggatga aatgatcatg agcgcaatcc gccatatgat attgcccgtg atcaccctgt
    42241 cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat cagcactatc gaagtgtatg
    42301 accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc gcgctttact attttgcgtc
    42361 gccacgttct gcataacgcg ttaccgccgg ttattcctcg cctgggctta cagttttcca
    42421 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    42481 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    42541 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    42601 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    42661 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    42721 tacggctgcg cggggctggc tgtactgtgt atgtttggcg gctggtttgc gccttacggc
    42781 atcgaccagc aatttctcgg ttaccaatta ttgccgccat catggtcgcg ctatggcgaa
    42841 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    42901 ggagctgcgc caaccgtggg tggcgcattt gtggtgacac ttgccgcgac gatctgtggc
    42961 ctggtactgg gcaccttcgc cggggcgacg cacggcctcc gctcagcggt gcttaaccat
    43021 attctggata ccttactggc gatcccttcc ctgctgttgg caattatcgt tgtagcgttt
    43081 gccggaccga gtttgtccca cgctatgttt gctgtctggc tggcgctgct gccgcgtatg
    43141 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    43201 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc taacatcacc
    43261 gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg caattctcga tatcgccgcg
    43321 ctgggctttc tcgatcttgg cgcacaactc ccctcgcctg aatggggagc gatgctcggt
    43381 gatgcgctgg aactgattta tgtcgccccg tggactgtca tgctgccagg tgcggcaatt
    43441 atgattagcg tgctgttggt taacctcctt ggcgacggtg tccgtcgtgc aattattgcg
    43501 ggggtggaat aatgccatta ctcgatattc gtaacctgac tattgaattt aaaaccggtg
    43561 atgagtgggt taaagccgtt gaccgggtaa gtatgacctt aaccgaaggt gaaatccgcg
    43621 gtcttgttgg tgaatccggt tccggcaaaa gtttgattgc gaaagcgatt tgtggggtga
    43681 ataaagataa ctggcgtgtt actgcagacc gtatgcgttt tgatgatatc gatttgctgc
    43741 gtctctccgc acgcgaacgg cgcaaactgg ttggccacaa cgtgtcgatg attttccagg
    43801 aaccgcagtc gtgtctagat ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc
    43861 cagcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca
    43921 ttgaactgct gcaccgcgtg ggaattaaag atcataaaga tgcgatgcgc agttttccct
    43981 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc cattgcactg gcgaatcaac
    44041 cgcgtctgct gattgctgac gaaccgacaa actcaatgga gccaacaacc caggcgcaaa
    44101 tctttcgcct gctgacgcgt ctaaaccaaa acagcaatac cactattttg cttatcagcc
    44161 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    44221 cggtggaaac cgcgccgagt aaggagttag tgacgatgcc acatcatcct tatacccagg
    44281 cgctgatccg cgcgatacca gacttcggaa gcgcgatgcc gcataaaagt cgcctcaata
    44341 cgctacccgg cgctatccca ctgcttgaac agttaccgat tggttgtcgt ctggggccac
    44401 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    44461 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg
    44521 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggttggtt tcgtcgtcag
    44581 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    44641 attggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagaa
    44701 cctaccagcg gcgagttatt gattgacgat catccactgc attttggcga ttattccttc
    44761 cgcagtcagc gtattcgcat gatttttcag gatccttcga cctcgttgaa tccccgtcaa
    44821 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccaacctgga accggaacag
    44881 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    44941 tactatccgc atatgttggc ccccgggcaa aaacagcgtc tgggtctggc gcgcgcgctg
    45001 atattgcgcc caaaagtcat tattgccgat gaagccctcg cttcactgga tatgtcgatg
    45061 cgttcgcagt tgattaatct gatgctggaa ttacaggaaa aacagggcat ttcgtatatt
    45121 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    45181 cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc tggcatcgcc gctgcatgaa
    45241 ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat tgacggcgga tgcgtggcgt
    45301 aaggaccgtt agtgttctgc acctcatcct gacaggggaa cccccctcag gatgaggtat
    45361 tcattacttt tgattgtcga ttctggtgag agcaaccagg aatgccagtg tataacacac
    45421 cgtaattgcc agactcattt ttaacagtgt ttctccgcca aatcccgaca atggcgcggc
    45481 tataccgccg aagacaaaca tgctcatccc catcaacgca gacgccgttc cagattcctg
    45541 cgtccctact gcactcatag cctctgcccc gccaacagtg ccgatgccgc tacaaaatgc
    45601 aatagtgaaa aataatgcca ccagtgccgg aatgggcaat tgtgtccatg cgcataagac
    45661 cgtgagcaat gcacacaaaa ttgccgctat caggccacct cgcaacaatg tcatcgcatt
    45721 aatccgtcgc gccagacgcg aaaagatcca tgaagcaatg ataagtccga tgccgttaag
    45781 gccaaacacc aggctaaatt gcattggact aaagccaaat tccttctgca acacgaacga
    45841 cgaagagccg atatacgcaa acaagcctgc cagcataaaa ctttgaatca ggcaaaagcg
    45901 cataaaacgg cggttctgca ccacgcttcg tccggttaat agcaatgatg agcccctttt
    45961 attttctggc aaggtctcat taataaataa cacgcagccc agcaacaata cggtgctaat
    46021 ttcagccatt acccagaata aagtgcgcca gtcaaaagtg ctaacaatgt acccgcccag
    46081 cactggcgac aacaccggtg ccaggccatt caccgtcatt aacagcgcaa aaaactgggt
    46141 caacgttact ccctgatatt tgtcacgagc aatagaacga gagagcaccg aaccacccgc
    46201 ccccgcgatc ccttgaataa aacgccagac caccagccag taaatattgt tcgttgtcgc
    46261 gcacaaaatg gaagaaacaa taaacaatag caacgacaag attaacggac gtttacgccc
    46321 tattttgtcg ctcagagggc caaataacag ttgtccgacg ccaagaccga tcagtgatgc
    46381 agtcagcgtt aattgcgtta tggtcgtggt tgccgccagt tgctcagaca tctccggcaa
    46441 agccggcaaa taaagatcgg tacacatcgg gccgataccg gctaaaagac caagaatcaa
    46501 tgcccatgaa agagagactc tagccatatt attccttact tgagtgatga catgccgccg
    46561 cccagcgact gccagagcgt gattcggttt gtaaaatcag tcagttgcag tgccagcagc
    46621 tcctgctgtg cgctccacaa gctgcgctgc gccgtcaaca cggtcaggta atcaccaaca
    46681 cccgtctgat agcgacgcaa gccaacatcg accgtttgtt gctcagcttt aacatactgg
    46741 cgctgtgcat ccagttgctc ttcaagcgtg gtccgccgcg ccagtgcgtt agcgacatct
    46801 ttaaatgctc tctgaacggt tttttcatag gtggcaatga gtccacgctt ttgtgcttct
    46861 gcgtaacgaa gctgcgccag attacttccg ccagtgaaca gcggtaatgt gaccgatggc
    46921 gtaaatgacc agatctgcat tccgtggctg aacaacgacg acaatgcgtc actgccaaca
    46981 ccagcgctgg cagtcagcga aatcgtcggg aaaaagttag cccgcgccgc gccaatatcg
    47041 gcattggcgc tcttcaaatt atgttccgct tcctggatat ccgggcgacg cagcagaacg
    47101 tcagatgaca ctcccgcagg aaccagactt atcatctgtt ccggcaggct ttccagtgtt
    47161 cccggtagca aattttcctc aagcgttgta cctgccagta agttcagcgc atttttatcc
    47221 tgcatcactt gtgtctgata gctggcaacg ctggcgcgcg cttgctgata aacactcatc
    47281 gcctcactga catctgtcgc tgccgccgtg ccaacctgtt gctggcgctg gataattttc
    47341 aatgagtttt ctgcactggt catcgtttct tttgccaggg ccaggttgct gttatcggcc
    47401 gccagtgtca gccaggcggt gctgatttca gcaatcagcg ttaaacgggt gttctgggct
    47461 gtaaattcac tggcaagcca ggtttctcgc gccgcccgag ataaactctg attacgacca
    47521 aacagatcta acgtaaaact ggacaccgtg ccgtcagcct cggcagtggt ttccgtacca
    47581 ttcgccagcg aacgactgcg ggttgaactt aaggccgcat tcaccgtcgg gaataacgac
    47641 gcattagttt gcccatagag cgcccgtgct gagtcaatat cagcaatcgc tttttgcaca
    47701 tcgcggttac tgttcagtgc aatagtcacc acctgctgca atcggggatc gtggatcacc
    47761 tgctgccagt catggctgat tgcctttccc tggccctgtg ctcccggcaa ggtcgccggg
    47821 attggggatt caggcgtgct gtagtgggga tcgagcgaga cacatcccgc actcaacagc
    47881 accagaaaaa taagagaacg acgcaacata acttactcct gacggcgcga tttaccggca
    47941 aacaaacgct tcaccagtac aaaaaacaga ggaacaaaga aaatagccag caacgtagcg
    48001 gtcagcgtac cgccaataat gcccgtacca atggcgatgc ggctgttcgc ccctgagccg
    48061 gttgctatcg ccagcggcat taccccccgc aataaacgcc agcgaggtca tgatgattgg
    48121 gcgtaaacga gtctgcgccg cgcgtaatgc cgcacgactc aaggaataac cctctgcaac
    48181 cgccgcttca gcaaattcca caatcagaat ggcgtttttc gacgacaagc cgatagtggt
    48241 taatagcgcc acctggaagt aaacatcgtt gtttaaatca cgcatccagg ccgccagcgc
    48301 cgcgccaaga agccccagag ggatcaccag aatgaccgag aacgggactg accagctttc
    48361 gtacagtgct gcaaggcaga ggaaaaccac cagaatagaa actgcataca gactcaatgc
    48421 ctgaccgctg gccagtttct cctgcaatga caaaccactc caggcccagg ttgttcccgc
    48481 aggaagactg tttgccagtt cctccatttt tgtcattgca tcgccggaac taaagccagt
    48541 cgcattttcc ccctgaattt cataggctgc agaaccgttg tagcgcacca gtctttccgg
    48601 tccatacagc cagcgggtgg tcgcaaaagc agagaatggt gtcatggcgt tatcactacc
    48661 gcgcacaaac catttaccta agtctgacgg cgcggaacga aattcactgt cgccctgaat
    48721 gtagactttt ttcacacgac cacggtcaat aaagtcattc acataagtac cgccccacgc
    48781 actggacagg gtgtcggtga catcattcaa actcaaccct aatgacaccg ctttattact
    48841 atcaatatct acctgcaatt gcggcatttg tggcaaatca ttggcacgaa cagaatgtaa
    48901 ctccggactt tgattcgctt tttctatcaa ctgattacgc atttgcagta gtgtttcgcg
    48961 atcggttcca ccgttagcta acaattcaaa cgtaaaacca ttgctttgcc ccagcccatc
    49021 aacggctggc ggcgtcatcg caaataccgt ggcatcacga attgtgccca gctctttggt
    49081 tgcccgtagg gcgatagcct gggcggtgtt ttctgcccct ttacgttgag accagttttt
    49141 caacgaaaca aacgccatcc cggtgttctg tccgctgccg ctaaaactga aaccatcaac
    49201 ggtaaaaatg acatcggtat ttgctttctc gttaatcagg aaccagtcaa caatctggcg
    49261 attcacttct gctgtacggg cagcggtcgc cccggccggc agcgtgtact gcaccatgat
    49321 ttcgccctgg tcttcagtgg gtaagaaact gcccggcagt ttccacatca tcagggccat
    49381 tccaccaccc agtacgagat aaagccccat cgttcgagca gcacggcgca ggacataaat
    49441 tacgcctcgc tgatatttat cttcagtacg gcggtaaaag cggtcaaatg cgccgaaaaa
    49501 gcctttttta tgtggcggaa catgctggag gacggaaccg cacagggcgg gagtcaaggt
    49561 caatgccacc accacggaaa gcagcattgc ggagataatg gttatcgaaa actgacgata
    49621 aattaccccc gtagatcccc caaagaaggc cataggcagg aatacggctg acaacaccag
    49681 cgcaatggca accagtgcgc cagaaatctc gcccattgat ttttccgtgg cttcacgcgc
    49741 gggcaaccct ttgtcacgca taatgcgttc gacgttctcc actaccacaa tggcatcgtc
    49801 caccagcaag ccgatcgcca gcaccatagc aaacaacgtc agggtattaa tcgaatagcc
    49861 aaacaacgca agaacgccga atgtgcccag caaaacgacg gggacagcta acgccggaat
    49921 cagtgtggca cgcaggtttt gcaggaataa atacatcacg caaaccacca atacgatggc
    49981 ttcaaacagc gtctgaatta cgtcctctac agagatttta ataaattcag tgctgtcttt
    50041 cgggtaagcg atgtcgtatc cctgcggcat gttccgctgg aattcggcaa tcttatcctt
    50101 gaccagcgtc gccgtattca gcgcattcgc accgggcgaa agcattacag caaccccggc
    50161 agacgggtga ccgttaagtt tgccgatagc ggtataatct tcactgccca tctcaacgcg
    50221 agccacatct tttatacgaa caactgcgct gtctgactgg cttttcacga taatattttt
    50281 gaactgatcc accgtctgca agcgagactg cgcacgaacc gttgcggtca gttgctgagt
    50341 attcggtgaa ggcaatgccc ctattttccc ggcagtaacc tgcacgtttt gcgcttcaat
    50401 agcactttgc acgtctgaag gcattagcga gtaagacgcc agtttggccg ggtcaagcca
    50461 gatgcgcata gcgtattccg cgccaaagac ttgcagactc cccacacctt caacacgcgc
    50521 cagcgggtcc tgaacgttac taaccaacca gtcggcgata tccgaactgg aggctttgtc
    50581 agtggtgtca tacacggcag caatcagcaa aaagttgctt tgtgatttct ccaccgtaac
    50641 acccgtttgc tgcacttcgc tgggtaggcg cgactccgcc tgctgaattt tattctgcac
    50701 ctgtgcagta tctggatcgg taccttgttc aaaggtcaca ttaatactga ccgaaccatc
    50761 agagctgctg gttgagctga aatagagtaa attatcaagc ccagtgagtt gttgctcgat
    50821 gacctgagtc acgctgttct ccagcgtttc ggcagaagca ccagtataag tggctgaaat
    50881 tttaatggtc ggtggcgcaa cgtcaggata ttgcgcgacg ggcaatgtgc gaatagccag
    50941 aatccccgcc aacataatca aaatggcgat aacccaggca aagaccgggc ggcgaacaaa
    51001 aaagcgcgaa aacatcaggc gttgcctcca ttagcctgaa cttcaacagc cttgaccgtc
    51061 tgccctgaag tgactttggc agaaccttca acaatcagtc ggtcgccgct gtgcaggccg
    51121 tttagcacca gccatttatc accatatgtt tctcccgttt cgagcgttcg ctgctctact
    51181 ttattgtctt tattcaccac cagcgcagtt gcattgcctt tagcatcgcg cgtgacgccc
    51241 tgttgcggcg cgagaatagc gtcttccatc acgccttcat cgacacgagc gcgaacaaac
    51301 attcccggta ataattgctg ttgtggattg gggaaaatcg cccgtaatgt caccgaaccg
    51361 gtagactcat ctacagcgac ttcggtgagt tccagacgcc ctttttcgct gtaggttgtg
    51421 ccatcttcca gaataagtga gacgctcatg gtgtcactgt tggtcgccag tgactgttta
    51481 cgcagacgta ataaatcgac actggagcga gtgaggtcga catacattgt atccagacca
    51541 cgaatagtcg ttaacgctgt atcttgcgac gcggtcacca gtgcgccagg ggttaccgac
    51601 gaaatgccaa tgcgccctga aatcggtgcg gttaccgtgg tccagtcaag attaatgcgc
    51661 gcagtttcca gtgcggcttt tttcgccgct acactggctt tatcttgtgc acaggtagac
    51721 tgagcatcat cagcatcctg ttgtgaaaca ccgttctctt tcaccagtcg ggcataacgc
    51781 tgcgctttct ggcaatcggc ttttaccagt gcctgtgctt gttgtaatgc tgcccgagct
    51841 tcattccatg cagcctgata actggccgca tcaatctggt agagcggctg tccagccttg
    51901 accagatcac cttccttaaa taagcgtttc tggataattc ccccaacctg cggacgtact
    51961 tcggcactga gcgcagcact ggtgcgtccg gttaattcac tgactaccga gaccggttga
    52021 gatttgactg taacaacgcc gacttctgtt tctgacgggg atgaattgtt actttgcgta
    52081 ttatcacaac cagataagag cagcattgcc actacagatg ttgctatata cttcattatt
    52141 ttctccaggg tgagaaatac cctcctcatt aaaattaatg aggatatgtc agggatattc
    52201 agttataacg aacccgcatc gcgaaatgaa tttcgcaatg aaataagggg gattaatatc
    52261 ccccgctatt ggttaattct gttcttattt atttatcaac atactaacaa tatcctgata
    52321 tatttcctgc agatgttgtg gatcgctttt ttgcggtgtt aaacgacgat aaaccgtacc
    52381 ttccatcatg gtagcaaaca cttcaacgca gcaacgaatg tgctcctcgc taagatgggg
    52441 atgcattctt ttcatatgtg cacaaccgtt agcgaacatt ctggcgtcag cttcttcaag
    52501 cattgccata acccgcgggt tgcgtgtggc ttctgcagcc acttccagca tcagcgcgtc
    52561 gtcgtcctca tttaaggatt tacgcaaggc aaggacttcc gggaagtgat cggtacgcgc
    52621 gtcaatgtcc atttgggcaa tacgaaagtc gatgatacgg cgaaccatct cttcaataat
    52681 ggcgtctttg ttggcgaagt aacgatatat ctgacccacg ctaagctgag cttcgctggc
    52741 aatttgcgac atactggcgg catgaaaccc gcagcgacga aaacaccgac gggcggcgtt
    52801 aatgatttca tcctgacgct gtttgtggcg tatttccagc ttgctggtca tttctgtctc
    52861 ctggcctatc acttggtgag aatgatcgtt ctcatttttg ctgattatac gtttctccaa
    52921 agtaatggaa agtattttta tcttattcat ggtgcatatt ctgataatga agagattgtg
    52981 gagattctgt gaagatagtt gacaatatta agttggggtc aattcatcag aaaggctgct
    53041 acttgttgtc gccagttctg ttttcatcat caaacttatc ggagctaata cgaatagttt
    53101 catagtcaga ttctataaga tatttcccca acctacccct tgcaggcgtc aagagtagat
    53161 aacgcaaaat gctctgtacc gcagcttctc tccggtacag aaagcgcaac tatagccacc
    53221 cacagccagg ttgattataa taaccgttta tctgttcgta ctgtttacta aaacgacgaa
    53281 tcgcctgatt ttcaggcaca acaagcatca acaataagga ttaaagctat gggttttctt
    53341 tccggtaagc gcattctggt aaccggtgtt gccagcaaac tgtccatcgc ctacggtatc
    53401 gctcaggcga tgcaccgcga aggagctgaa ctggcattca cctaccagaa cgacaaactg
    53461 aaaggccgcg tagaagaatt tgccgctcaa ttgggttctg acatcgttct gcaatgcgat
    53521 gttgccgaag atgccagcat cgacaccatg ttcgctgaac tggggaaagt ttggccgaaa
    53581 tttgacggtt tcgtacactc tattggtttt gcacctggcg atcagctgga tggtgactat
    53641 gttaacgccg ttactcgtga aggcttcaaa attgcccacg acatcagctc ctacagcttc
    53701 gttgcaatgg caaaagcttg ccgctccatg ctgaatccgg gttctgccct gctgaccctt
    53761 tcctaccttg gcgctgagcg cgctatcccg aactacaacg ttatgggtct ggcaaaagcg
    53821 tctctggaag cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg tgtgcgtgtt
    53881 aacgccatct ctgctggtcc gatccgtact ctggcggcct ccggtatcaa agacttccgc
    53941 aaaatgctgg ctcattgcga agccgttacc ccgattcgcc gtaccgttac tattgaagat
    54001 gtgggtaact ctgcggcatt cctgtgctcc gatctctctg ccggtatctc cggtgaagtg
    54061 gtccacgttg acggcggttt cagcattgct gcaatgaacg aactcgaact gaaataatcg
    54121 ttctgttggt aaagatgggc ggcgttctgc cgcccgttat ctctgttata cctttctgat
    54181 atttgttatc gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat aacgatttcc
    54241 tttacgacca aggagcgccc atggaacaac gccacatcac cggcaaaagc cactggtatc
    54301 atgaaacgca atccagtact acggagtatg acgttctgcc tctggtcccg gaagccgcaa
    54361 aggtcagcga tccctttcta ctcgacgtga tccttgaaaa agaaacgctg gcccccttcc
    54421 tttcatggct ggaccctgcg cgtgttcttg cagtggagtt gttccctgac cagcttaccg
    54481 tgacccgttc acagaccttc accgcttatg aacgcttgtc gacggccctg acggttgctc
    54541 aggtttgcgg cgtccagcgg ttatgtaact actattcggc gcgacttacg ccgctccccg
    54601 ggcctgattc caccagggaa agtaatcatc ggttggcaca aatcacgcaa tatgcccgcc
    54661 aactggctag ctcgccttct attatcgaca accgatcgcg ccagcatctg aatgacgtcg
    54721 gtcttactgc ctgggactgt gtgatcatta accaaatcat tggttttatt ggctttcagg
    54781 cgcggacaat tgcgacattt caggcttatc tcgggcatcc ggtacgctgg ttacccgggc
    54841 tggagataca aaactacgcc gacgcgtcac tgtttgctga tgaatcatta cgctggcgaa
    54901 gcagctatga ggtggaaaaa ctacctgaag aacacacaaa aagttcaact gcagaacttt
    54961 gccaactggc cgaaatactc tctctccacc ctatttcact ttcccttctc gaaaggttgt
    55021 taaacagcac acgggttaat acacagccgg ataatcagct tgcggcgttg ttatgcgcgc
    55081 ggataaatgg cagtcctgct tgttttgccg cctgtatgga ttcatcaaat gaatataaaa
    55141 aaatcagccc ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct gaccgtcatt
    55201 ctgttgagcg cgctaccgtt caggcgatac aatggctgac ccgagcaccc gatcgcttta
    55261 gcgccgccca gttcagccct ttactcgaac acgaaaaatc atcaacgcag attattaatc
    55321 tgctggtatg gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg ttgggtgaga
    55381 catattaacc ttgccgcgtc agacagattc gcgtaaaact gtcagccgct ctaatggcca
    55441 ccaaaataga caattatgtt tcaggacaac ccgctgctag cgcagcttaa acagcaactg
    55501 cattcccaga cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg ttttggcttc
    55561 ctggaagtcg acgcgcaaaa aagttatttc attccgccgc cgcagatgaa aaaagtcatg
    55621 catggcgacc gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc cgcagagcca
    55681 gaagaactgg ttgaaccgtt tctgactcgt ttcgtgggta aagttcaggg caaaaatgac
    55741 cgtctggcca tcgttcctga tcatccactc ttaaaagatg ccattccttg ccgcgcagcc
    55801 cgtggtctga accacgagtt taaagaaggc gactgggcag ttgccgaaat gcgccgtcat
    55861 ccgctgaaag gcgatcgttc tttctatgct gaactgacac aatacatcac ttttggtgac
    55921 gaccactttg taccgtggtg ggttaccctt gcgcgccata atctggaaaa agaagcacca
    55981 gacggcgtcg ctaccgaaat gctcgatgaa ggtctggttc gtaaagatct gaccgcgctg
    56041 gattttgtca ccatcgacag tgccagcaca gaagatatgg atgacgccct tttcgctaag
    56101 gcgttgccgg atgacaaact tcagctgatt gtggcgattg ccgatccaac cgcgtggatt
    56161 gctgaaggca gcaagctgga caaagccgcg aaaattcgcg cattcaccaa ctatctgcct
    56221 ggcttcaaca tccctatgct gcctcgcgag ctttctgacg atctctgctc actgcgcgcc
    56281 aatgaagtcc gcccggtact ggcatgccgc atgacgctct ccgctgatgg caccattgaa
    56341 gataatatcg aattctttgc cgccaccatc gaatccaaag cgaagctggt gtatgaccag
    56401 gtttctgact ggctggagaa taccggtgac tggcagcctg aaagtgaagc aattgccgaa
    56461 caagtccgtt tgctagcgca aatttgccaa cgccgcggcg agtggcgtca taaccacgca
    56521 ctggtgttta aagatcgccc ggattaccgc tttattctcg gtgaaaaagg tgaagtgctg
    56581 gatatcgtcg ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc gatgattgcc
    56641 gctaacattt gtgcagctcg cgtactgcgc gataagctcg gttttggtat ctataacgtg
    56701 catatgggct ttgatccggc gaatgccgac gcgctggcag cgttgctgaa aacgcacggt
    56761 ctgcatgtcg atgccgaaga agtgctcacg ctggacggtt tctgcaaact gcgtcgtgaa
    56821 ctggatgcgc aaccaactgg tttcctcgac agccgcattc gtcgcttcca gtcatttgct
    56881 gaaattagca ctgaacccgg tcctcacttt ggcctcggtc tggaagcata cgccacctgg
    56941 acttcgccga tccgtaaata tggcgacatg atcaaccacc gtctgctgaa agcggttatc
    57001 aaaggcgaaa ctgcgacgcg tccacaggat gaaatcactg tccaaatggc cgagcgtcgc
    57061 cgtctcaatc ggatggcaga acgtgatgtt ggtgactggt tatacgcacg cttcctgaaa
    57121 gacaaagccg ggaccgacac ccgtttcgca gcggaaattg tcgatatcag ccgtggcggc
    57181 atgcgtgttc gtttggttga taacggcgct atcgccttta ttcctgcacc tttcttacac
    57241 gctgtgcgcg atgaactggt ttgcagccag gaaaacggca ccgtacaaat taaaggtgaa
    57301 acggtttata aagtaactga cgttattgac gtcaccattg ccgaagtccg catggaaacc
    57361 cgcagcatta ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc cttatgaatg
    57421 ggccgtttat ttccccgctc taccttcatc atatcccggc agttttttaa ctgactttcg
    57481 tttgaaaact ggcgtttttc caaaactaga ttacaaataa atatataaaa aaaacaatgt
    57541 gtttagccgc cgttaatttc atgtaagtca tgttgttcct tttatttaac cgcggactcc
    57601 gctgttaacc ggaggatatg catcatgaaa accgttaggg agtccacaac gttgtacaac
    57661 tttctcggat cgcacaatcc atactggcgg ttgacggaaa gcagcgatgt tttgcgcttt
    57721 tctaccaccg aaaccacaga acctgaacgt acgttgcagt tatctgccga acaggctgct
    57781 cgcatcaggg aaatgacggt catcacctcc agcctgatga tgagtctgac cgtcgatgag
    57841 agcgatcttt ctgtgcatct ggtaggacga aaaatcaata aacgggaatg ggctggcaac
    57901 gcgtctgcat ggcatgacac accagcagtt gctcgtgatt tatcacacgg actttccttt
    57961 gctgagcagg tagtttctga agcacattcc gcaatagtga ttctcgacag ccgggggaat
    58021 atccaacgct tcaatcggtt atgtgaagat tacacagggt tgaacgaaca cgacgtcatt
    58081 ggccaaagcg tgtttaaact gtttatgagc cgtcgtgaag ctgcggcatc caggcgcaat
    58141 aaccgtgtat tttttcgaag cggcaatgca tatgaagtcg aactgtggat accaacacgt
    58201 aaaggccagc ggctgtttct gtttcgcaat aaatttgtcc acagcggcag tggcaaaaac
    58261 gagatttttt taatctgttc cggcaccgac attaccgaag agcgccgcgc tcaggaacga
    58321 ctgcgtattc tggcaaatac cgacagtatc accggactgc cgaatcgtaa cgcaatgcag
    58381 gagttaatcg atcacgctat taatcaggca gataacaata aagttggggt cgtgtatctt
    58441 gatttggata attttaaaaa ggtcaacgac gcctatgggc atttatttgg tgaccagtta
    58501 ttacgcgacg tgtcattggc tattttaagt tgtctcgaac atgaccaggt gttggcgcgt
    58561 ccaggtggcg atgagtttct ggtactggca tccaacacct cacaaagcgc gctggaagca
    58621 atggcatcac gaattttgac ccgcttacgg ctcccctttc gcattggttt aattgaagtt
    58681 tataccagct gttcagtagg tattgcactc tctcccgaac atggttcgga cagcgcggct
    58741 attattcgtc acgccgacac agcaatgtac acagcaaagg aaggcggacg aggacaattt
    58801 tgcgttttta ccccagaaat gaatcaacgg gtatttgaat atctctggct ggataccaac
    58861 ttgcgtaaag cactggaaaa cgatcagttg gttattcact atcaacccaa aatcacctgg
    58921 cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc agtcacctga acgtgggttg
    58981 attccaccgt tggacttcat ttcctacgca gaagagtcag ggctaattgt gcctttaggc
    59041 cgttgggtga ttctcgatgt cgtacgccag gtggcgaagt ggcgggataa aggcattaac
    59101 ctgcgagtgg cggtaaacat ttctgcacgt cagctcgccg atcaaaccat tttcaccgcc
    59161 ctgaaacagg ttctccagga actcaatttt gaatactgcc ctattgatgt tgaattgaca
    59221 gagagttgtc tgattgagaa tgatgaactg gcactgtctg ttattcaaca atttagccaa
    59281 ctaggtgcgc aagtgcatct ggatgatttt ggtaccgcct actcttcact ttcgcaactg
    59341 gcgcgctttc cgatcgatgc catcaaactt gaccaggttt ttgttcgaga tattcacaaa
    59401 caacctgtct cgcagtcact ggttcgggca atcgtcgctg tggcccaggc attgaatctt
    59461 caggtgatcg ccgaaggtgt agagagtgca aaggaagatg cttttttaac caagaatggg
    59521 atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc ccgccgtcgc cttcgaacgc
    59581 tggtataaac gctatctgaa gcgcgcataa gtagatgaat atatatactt agcgctatat
    59641 tccgtcatta tatatcgtat tcccggatga taattagccg ttgctgttaa acacttctct
    59701 tttagcggca atttttgcat tattcatttt cccccttttg ctataaggat gtccttgatg
    59761 tctgaatttg atgcccagcg cgttgccgaa cgtattgata ttgtgttaga tatccttgtg
    59821 gcagacgatt accactctgc gatccataat ctcgagatac ttaaagcaga actcttgcgc
    59881 caggttgctg aatctacgcc agacatcccg aaggcaccgt gggagattta acaatcgtgc
    59941 cttcaggttt tcctatttga ggatgcccgg cgcgtcagta tttttttatt tagtattata
    60001 acgttataag aattacagcg atgaattccc gacaacaaac tattctacag atggtcattg
    60061 accagggtca ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca
    60121 ttcgccagga tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca catggctttg
    60181 cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc
    60241 gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc tttatcgaaa
    60301 atggcagcag caatgccctg ctcgcccgga ctctggggga gcagaagaaa aatgtcacta
    60361 tcatcacggt cagcagctac atcgcgcatt tgctgaaaga cgcgccttgt gaagttattt
    60421 tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt tggcccgttg acacgccagt
    60481 gcatccaaca ggtacatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa
    60541 ctggattcac cggtcgcgat atgatgcgta ccgatgtggt gaatgccgtg ctggaaaaag
    60601 agtgcgaagc gatagtcctg actgacagct cgaaatttgg tgctgtacat tcatactcca
    60661 tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc
    60721 tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa ccatttccct
    60781 tctctacgga tgatttgcag tttgacaaat catccgctct aagatgattc ctggttgata
    60841 attaagacta tttacctgtt attaacactc tcaagatata aaattattat cagcgatata
    60901 acaggaagtc attatcacct gcgtgatata accctgcgcg cgagcggatt tcacggaata
    60961 atttcaccag gcttattctt agctattata gttatagaga gcttacttcc gtgaatcata
    61021 aattcaggag agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc tgttctggca
    61081 attaccctgg caatgtctct gagtgcctgt tctaactggt ctaaacggga ccgcaacacc
    61141 gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg
    61201 ggtacattag gtggcgcagc tgtcggtggt gttattggtc accaggtcgg taaataatcg
    61261 taaataaatg tcgattatcg actcgtaatt ataatacgct tgattctgtt cagtacgaaa
    61321 taactgaata atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta
    61381 tgttaaccgc ctgcgagttt taccttcatc cctttcgctt caagtagtga ttttaataaa
    61441 tcacgcttat cgccctggat ttcaataact ccatctttaa ctgctccgcc gcagccgcat
    61501 tttttcttca gttccgctgc aagttttgtc agttcggcat catcgagatc gataccggta
    61561 atcaggcaaa cgcccttacc tttacgtcca ctggtctgac gctgaatacg caccacacca
    61621 tcgcctttag agcgtactgg ggccgctttg ggctcatcaa tacgcccggt ttctgttgag
    61681 tagaccagac ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg
    61741 ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat
    61801 caacaccagc cgccaacgcc tgttctggcg tcataatgcg gcgctggtca ccagcttcac
    61861 tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc ctgaccgaat acctgtttaa
    61921 agcgcacagc ttcctgagca gaacacacca caccatcaag gccacatttt tgcgtcagtg
    61981 ctgccagacg ttctgcataa tctgcaggtg acagtgtcac gccaagatcg gccaggtcgc
    62041 tggcttccat gctggtcaac actgtcacag caatcaaaag cggtgcatct ttgccaaacg
    62101 gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc accagaggca tgaacattca
    62161 ccatccacac gcctaagtca gctgcagcag cgacagcgtg cgctgcagtg ttggggatat
    62221 cgtggaattt caggtcaaga aagatatcaa aaccacgctg ttgaagttcg cgcacaaact
    62281 gtggcccaaa caatgtaaac atctctttgc cgaccttcag acgacaatcg cgtgggtcga
    62341 tcttgtcgac aaaggccagc gcggcatcac gattatgata atcaagggca acaaccacag
    62401 gagaattcgt aacagcgcgg gaagaagatg aagcagttaa cgtcatgacc agaccttctt
    62461 gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc gagcattcta cccgtggaaa
    62521 taaaaaatta acagttgcga tttctcccct ggcaggcttt ttctgcgccg ccgttgccgg
    62581 agcacaatgg gacataaata actagtatgt tgtaactaaa gtcggttttt ttaaaaaatt
    62641 acaggccatc aagaccgcga atcggtttaa tagttgacca ggcacgacaa gacggacaat
    62701 gccagtagag ggtgtatgcg gtaaaaccac atttctggca gcgataacga ggcttactac
    62761 gtaccttctc gccaaccatg tcacgcagca ccatcaggct ctctttggca cgcccttctt
    62821 ccgcttcatt taagtggtaa tccattaact tatggaacac acgcatggtc ggatgacgct
    62881 gaagctggcg cgtaatatag acctgtgcgg cctcactacc gtcgcgcgct tcgatgatat
    62941 cagcaagcat caattcagca tcggcaccgg tgttctcttc caccgcgcgc tgcaggaatt
    63001 ctgcccattc ggcagtttta cccaactgct gatagcaggt ttgcagcatt tccagcgttt
    63061 cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa actttcgacg gctttggcgt
    63121 attctccttt cgccataaac acgcgtccca tcatgatgga tacgcgggcg ctgtttttat
    63181 ctgccgccgc cccttttttt agcaaggtca tggcacgatc gagatcgtcg ctggccatat
    63241 gctgcagggc taactcacag tagaaatggg caatttcgac gcgctgttta tctttaccca
    63301 gcttcaccag gcgttcggca acatcaattg ctttctgcca ctcactggta gcctggtaga
    63361 tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt ttcatcggtc agctgattga
    63421 acatgtcttc cgcgcggtca tataacccgg cggccatgta atcacgcccc agttgttgaa
    63481 tcgccaacag acgctgttca taggtcagcg aggcgctttc cattagggtc tgatggatgc
    63541 gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt tccgagcgta aggtgggctt
    63601 caacggtgcc tgtatcctct ttaagcatat cgagaaacag gtctaccgct ttatcctgtt
    63661 gattactaag caggaagtta acccccgcta cgtaatcacg cgacaagcgg ttggcttcat
    63721 cttgcttgtt ttgttgcgca cttctgcggc ccatatacca gccataggca gcggctacag
    63781 gcaaaagcag aaacaacaac tccagcatag aaagttattc cttcgccgct gacgagtgct
    63841 gcactacagc cacgtcagtc gcgggtgaaa gctggttttc cagtcgcttt attttacgtt
    63901 cagcgcgcac cagggaaaca cgaactcgca gccagaacag gccacaaatc aaccaaccga
    63961 tagcaaaacc cgcagcaaac aataccgcca gcaatgtgga aatacggtac tccccttgcg
    64021 ctaacagata attaaacgtc acctgttgat cgttctgcgc acccaacgtg accgaaatca
    64081 cgaagatcgc taacaccagt aagaaaatga gtaaatattt cacattactt cccgttatgt
    64141 ggttagagcg aataaattat ggtcaactta ccgcaatagc cctttaaagt accattttcg
    64201 cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat ttaagggcta aattcggaaa
    64261 atcaaccagc gttaactttc ttgttctcgt tgcgctattt cgcgattttc ttccgcaggt
    64321 ggtgttaatg gcccgcaaat tcgttgcgca agccacgttg ccaccgccac cagtgcccac
    64381 gaaatcaacg tagctaccac cagatcgcgt ggccaatgca tcccgagcag caggcggctt
    64441 cccatgactc ccgttgccca gaccagcaag atagcaatgg ttaacgttcg ccgacgcggc
    64501 cacagcaaac caacggccag cagtgcccaa ctggcagcaa acatcgtgtg accggaagga
    64561 aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat attgtggaat atttttctct
    64621 tcagccaact gttctttcac tagatttccg cgttctgctc gctttaaagt gtagaactca
    64681 tcaaccggaa tatgatgtgt tttttccagc cagataacaa aaggtcgtgg ttcctggact
    64741 ttgtctttga tccaggattt aacgccttgt cccacaagga ttgcggccgc cagaatggca
    64801 aataatacaa aggcagcctt aatgcgaaaa cgcagacacc agagaaacca gccgaataaa
    64861 atcaaatgtg taatgacgcc ccagggctgg gtgacagttt cagtaaccca aaaagccgct
    64921 tttagtagcc aactttgttc tccaggttgc caacgccagc cagaaatcca tacggctact
    64981 ggcataacaa gcaatagtgc agctcccact gcggtacgtc ttgcaatcga acgcatggcc
    65041 tctccttttt gataagtccc acaatcataa ctgaaaatgc cagttccagg aaaaattgac
    65101 agatttgtgc cattccgtga acgatcgacg cgtcgtgatt aggtgaaccc cttctcgtta
    65161 tggcaaaata agccaataca gaaccagcat tatctggaga atttcatgca gcttaaacgt
    65221 gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc tgatggtggg atttgaagaa
    65281 ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg atatttccgg gcataccccg
    65341 gtacttgcgc gcgtccattc cgaatgtctg accggtgacg ccctgttcag cttgcgctgc
    65401 gattgtggct tccagctcga agcggcattg acgcaaattg ccgaggaagg ccgtggtatt
    65461 ttgctgtatc accgtcagga aggtcgtaac attggtctgc tgaataaaat ccgcgcttac
    65521 gcactgcagg atcaaggtta cgatactgta gaggctaacc accagttagg cttcgccgct
    65581 gatgagcgcg acttcactct ttgcgctgat atgttcaaac tccttggcgt caatgaagtc
    65641 cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga ccgaagcggg gattaatatt
    65701 gttgaacgcg taccattgat tgtaggtcgt aaccccaata acgaacatta tctcgatacc
    65761 aaagccgaga aaatgggcca tttgctgaac aaataaccct cttgcattgt gtaattaatt
    65821 tgcttgccgg aagcaaaata accggcaagc aaatacttgt tacttcaaca tattacgaat
    65881 gacataatgc aaaatgccgt cgttctggta gtaagtcaac tccgtcgcgg tgtcgatacg
    65941 acaacggcag ggtacgactt cctggctacc atccgcgcgc gtaagcgtca ccggaaccgt
    66001 cgcgccgggt tgtagatttt gcagatcgcc aatatcaatc ttctcttccc cggttagccc
    66061 taacgtttta cgcgttacgc cttgcggaaa ttccagcggc aggatgccca tgccaattaa
    66121 attcgaacgg tgaattcgtt caaacgattc ggcaatcacc acacgaatac caagcagacg
    66181 cggacctttt gccgcccagt cacggctgga gcctgatcca tactctttcc cggcaatcac
    66241 cgccagcggc gtttgctcct gcttatagcg catcgcagca tcataaatag agactacgtc
    66301 gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca ggcaccattt cattacggat
    66361 gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg ttaccacgcc gcgaaccgta
    66421 ggagttaaag tcttttcgct caacaccccg accttgtagg tatcgacccg ctgggctgtc
    66481 gggcttaata ctgcccgccg gagagatatg gtcagtggtg actgaatccc ccagcattgc
    66541 gaggatccgc gcaccgtgaa tatcttccac aggtgctggc gttgcctgca tttcatcaaa
    66601 gaaaggcgat aaacgaatat aggttgaatc ctcctgccat ccgtaggtat cggatcgtgc
    66661 gacgttaatt tctttccact ctgcagtgcc ttcaaagact tctgcgtact ctttgcggaa
    66721 catttctgtg gagacttgtt ctaccgcacg ggcaatttct tgtgccgatg gccagatatc
    66781 tttcagatat attggatcgc ctttacgatc atgaccaata ggctcagaag ccaggttgat
    66841 attcatattt cccgccagcg cataggcaac caccagcggc ggcgaggcca gccagttagt
    66901 tttaaccagc ggatggatac ggccttcaaa gttacggttg ccggacagca ctgcaccgac
    66961 ggttaaatcg ccttttttga ttgccgtttc gataggatcg ggcagcggcc cagagttacc
    67021 aatacaggtg gtacaaccgt atcccacaag gttaaacccc agttcgtcga gatacggtgt
    67081 cagtttcgct tttgccagat agtcagaaac gactttcgaa cccggtgcca gcgacgcttt
    67141 gacccacggt tgccgcttga ggccaagagt tacggctttt ttcgccagca agcctgcggc
    67201 catcagcaca cttgggttag aagtgttggt gcacgaggtt atcgcagcaa tgaccacagc
    67261 gccatcaggt aactgatact gatgtccgtt cataacataa tcgaccggct ggcgatcttt
    67321 atgcgtggca ttcacttcca gttcgttact ggcggcaaat gcttttggta catcgggcag
    67381 tgcaacgcga tcctgtgggc gtttaggccc tgccaggctc gcttcaacgt cattcatatc
    67441 cagatctaac gtactggtaa aaatcggttc atcgcccggg ttacgccaca tgccctgcgc
    67501 tttggcatat ttttcgacca actcgacctg atcttcgctg cgcccgctta aacgcatgta
    67561 atcgagggtt acagcatcga ttgggaagaa gccacaggtg gcaccatatt ctggcgacat
    67621 attggcaatg gtggcgcgat ccgccaacgg tagtgaatcc agaccatcac cataaaattc
    67681 gacgaatttc cccaccacgc catgtttgcg cagcatttgg gtaacagtga gaaccaggtc
    67741 tgtggcggta ataccttcac gtaattttcc ggtaagttta aagcccacta cgtccgggat
    67801 aagcatggaa accggctggc ctaacattgc ggcttctgct tcgatgccac ccacgcccca
    67861 ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc
    67921 cggataagca atccattcac cgtcctgcaa ttcgctccac actgctttgc cgagatattc
    67981 gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc
    68041 ttgctttccc catttcagga acacataacg ttcgtggttg cgctccattt ccaggcgtac
    68101 gttttcttca aatgcctcat catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac
    68161 caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg ccaccgaggc gtttaaccgc
    68221 ttcgcgcatt gccgccagat caacaacggc aggtacgccg gtaaagtcct gcatcagcac
    68281 ccttgccggg cggtaggcaa tttcacggtc agcatgggca tttttcagcc atcccgccag
    68341 cgcgtggata tcctcttcgg taaccgagtt accatcctgc cagcgcagca gattttcgag
    68401 caaaactttg agtgacttgg gtagacgggt gatatcgccc agtgatttgg cagcaagcgg
    68461 caggctgtag tagtggtaag ttttatcttt ggcctgcaac gcgtccttac tggcttctcg
    68521 tagggttgac gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag
    68581 ggtgaatatt aaagataaca caaacatcgc gtaacgattt gataacaacc caaattgata
    68641 aaagaggaga aaccttgtgg caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc
    68701 agtgagtaat gatccagagg agttcggccc agaagagcat cgacagagaa aatgcaccca
    68761 tccaggacca atatttcata tttgtgttga tcataggcta cacccttaac ggttattact
    68821 ttgtgttgag ataactcaac ctgttattgc tgcggtgtat taaaagccct ttaattcatt
    68881 aaaaatgggt aataataatg atgataatta ttgtttcggc tgatcatctt cagcaaacag
    68941 ttctataaag gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca
    69001 ttcccgtttc gtttcgggtt cattgtcact atgctggcat aatggtgacg actccagttg
    69061 ctcctgacca accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag
    69121 aaaagtgcag aaccaagaaa aaccgccagc caggctttac gttttaattc aggatcgcgg
    69181 cggggttcct gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc
    69241 aagacgttga acgattggtg taattaatca tacgatgaga caaatcctaa agaaacttta
    69301 gtcaaaaatc cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga
    69361 aatagatatt tgaaaactgg aat
//
LOCUS       DAZFKY010000003        66053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFKY010000003 DAZFKY010000000
VERSION     DAZFKY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..534
                     /gene="cmtB"
                     /locus_tag="ACHKCL_000167"
     CDS             91..534
                     /gene="cmtB"
                     /locus_tag="ACHKCL_000167"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5761216.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKCL_000168"
     CDS             562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKCL_000168"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5761217.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKCL_000169"
     CDS             1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKCL_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5761218.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKCL_000170"
     CDS             3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKCL_000170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5761219.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKCL_000171"
     CDS             4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKCL_000171"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5761220.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4732..5445
                     /locus_tag="ACHKCL_000172"
     CDS             4732..5445
                     /locus_tag="ACHKCL_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5761221.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5417..6094)
                     /locus_tag="ACHKCL_000173"
     CDS             complement(5417..6094)
                     /locus_tag="ACHKCL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5761222.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(6088..6765)
                     /locus_tag="ACHKCL_000174"
     CDS             complement(6088..6765)
                     /locus_tag="ACHKCL_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5761223.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6753..7460)
                     /locus_tag="ACHKCL_000175"
     CDS             complement(6753..7460)
                     /locus_tag="ACHKCL_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO5761224.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7461..8039)
                     /locus_tag="ACHKCL_000176"
     CDS             complement(7461..8039)
                     /locus_tag="ACHKCL_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5761225.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8062..8493)
                     /locus_tag="ACHKCL_000177"
     CDS             complement(8062..8493)
                     /locus_tag="ACHKCL_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5761226.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8865..9884
                     /gene="epd"
                     /locus_tag="ACHKCL_000178"
     CDS             8865..9884
                     /gene="epd"
                     /locus_tag="ACHKCL_000178"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5761227.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9934..11097
                     /gene="pgk"
                     /locus_tag="ACHKCL_000179"
     CDS             9934..11097
                     /gene="pgk"
                     /locus_tag="ACHKCL_000179"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5761228.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11312..12391
                     /gene="fbaA"
                     /locus_tag="ACHKCL_000180"
     CDS             11312..12391
                     /gene="fbaA"
                     /locus_tag="ACHKCL_000180"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5761229.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12455..12553)
                     /locus_tag="ACHKCL_000181"
                     /pseudo
     CDS             complement(12455..12553)
                     /locus_tag="ACHKCL_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12749..13609
                     /gene="mscS"
                     /locus_tag="ACHKCL_000182"
     CDS             12749..13609
                     /gene="mscS"
                     /locus_tag="ACHKCL_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5761230.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13748..14383
                     /gene="argO"
                     /locus_tag="ACHKCL_000183"
     CDS             13748..14383
                     /gene="argO"
                     /locus_tag="ACHKCL_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5761231.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14476..15216
                     /gene="yggE"
                     /locus_tag="ACHKCL_000184"
     CDS             14476..15216
                     /gene="yggE"
                     /locus_tag="ACHKCL_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5761232.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15383..16279
                     /gene="ygfI"
                     /locus_tag="ACHKCL_000185"
                     /pseudo
     CDS             15383..16279
                     /gene="ygfI"
                     /locus_tag="ACHKCL_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16276..17754)
                     /gene="scpC"
                     /locus_tag="ACHKCL_000186"
     CDS             complement(16276..17754)
                     /gene="scpC"
                     /locus_tag="ACHKCL_000186"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO5761233.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17778..18563)
                     /gene="scpB"
                     /locus_tag="ACHKCL_000187"
     CDS             complement(17778..18563)
                     /gene="scpB"
                     /locus_tag="ACHKCL_000187"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO5761234.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18574..19569)
                     /gene="meaB"
                     /locus_tag="ACHKCL_000188"
     CDS             complement(18574..19569)
                     /gene="meaB"
                     /locus_tag="ACHKCL_000188"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO5761235.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19562..21706)
                     /gene="scpA"
                     /locus_tag="ACHKCL_000189"
     CDS             complement(19562..21706)
                     /gene="scpA"
                     /locus_tag="ACHKCL_000189"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO5761236.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21910..22803)
                     /gene="argP"
                     /locus_tag="ACHKCL_000190"
     CDS             complement(21910..22803)
                     /gene="argP"
                     /locus_tag="ACHKCL_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5761237.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23186..23845
                     /gene="rpiA"
                     /locus_tag="ACHKCL_000191"
     CDS             23186..23845
                     /gene="rpiA"
                     /locus_tag="ACHKCL_000191"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5761238.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24100..25332
                     /gene="serA"
                     /locus_tag="ACHKCL_000192"
     CDS             24100..25332
                     /gene="serA"
                     /locus_tag="ACHKCL_000192"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5761239.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25561..25620
                     /gene="ibsC"
                     /locus_tag="ACHKCL_000193"
     CDS             25561..25620
                     /gene="ibsC"
                     /locus_tag="ACHKCL_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5761240.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25954..26013
                     /locus_tag="ACHKCL_000194"
     CDS             25954..26013
                     /locus_tag="ACHKCL_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5761241.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(26114..26716)
                     /gene="fau"
                     /locus_tag="ACHKCL_000195"
     CDS             complement(26114..26716)
                     /gene="fau"
                     /locus_tag="ACHKCL_000195"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5761242.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26737..26920)
                     /gene="ssrS"
                     /locus_tag="ACHKCL_000196"
     ncRNA           complement(26737..26920)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKCL_000196"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26962..27291)
                     /gene="zapA"
                     /locus_tag="ACHKCL_000197"
     CDS             complement(26962..27291)
                     /gene="zapA"
                     /locus_tag="ACHKCL_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5761243.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27459..28037
                     /gene="ygfB"
                     /locus_tag="ACHKCL_000198"
     CDS             27459..28037
                     /gene="ygfB"
                     /locus_tag="ACHKCL_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5761244.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28063..29388
                     /gene="pepP"
                     /locus_tag="ACHKCL_000199"
     CDS             28063..29388
                     /gene="pepP"
                     /locus_tag="ACHKCL_000199"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5761245.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29385..30563
                     /gene="ubiH"
                     /locus_tag="ACHKCL_000200"
                     /gene_synonym="visB"
     CDS             29385..30563
                     /gene="ubiH"
                     /locus_tag="ACHKCL_000200"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5761246.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30587..31789
                     /gene="ubiI"
                     /locus_tag="ACHKCL_000201"
                     /gene_synonym="visC"
     CDS             30587..31789
                     /gene="ubiI"
                     /locus_tag="ACHKCL_000201"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5761247.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31863..31946)
                     /locus_tag="ACHKCL_000202"
     CDS             complement(31863..31946)
                     /locus_tag="ACHKCL_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761248.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32237..33331
                     /gene="gcvT"
                     /locus_tag="ACHKCL_000203"
     CDS             32237..33331
                     /gene="gcvT"
                     /locus_tag="ACHKCL_000203"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5761249.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33355..33744
                     /gene="gcvH"
                     /locus_tag="ACHKCL_000204"
     CDS             33355..33744
                     /gene="gcvH"
                     /locus_tag="ACHKCL_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5761250.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33862..36735
                     /gene="gcvP"
                     /locus_tag="ACHKCL_000205"
     CDS             33862..36735
                     /gene="gcvP"
                     /locus_tag="ACHKCL_000205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5761251.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36901..38334)
                     /gene="bglA"
                     /locus_tag="ACHKCL_000206"
     CDS             complement(36901..38334)
                     /gene="bglA"
                     /locus_tag="ACHKCL_000206"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5761252.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            38379..38690
                     /gene="yqfB"
                     /locus_tag="ACHKCL_000207"
     CDS             38379..38690
                     /gene="yqfB"
                     /locus_tag="ACHKCL_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5761253.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38854..39513
                     /gene="yqfA"
                     /locus_tag="ACHKCL_000208"
     CDS             38854..39513
                     /gene="yqfA"
                     /locus_tag="ACHKCL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5761254.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39590..40570)
                     /gene="ygfZ"
                     /locus_tag="ACHKCL_000209"
     CDS             complement(39590..40570)
                     /gene="ygfZ"
                     /locus_tag="ACHKCL_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5761255.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            40813..41079
                     /gene="sdhE"
                     /locus_tag="ACHKCL_000210"
     CDS             40813..41079
                     /gene="sdhE"
                     /locus_tag="ACHKCL_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5761256.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            41060..41467
                     /gene="ygfX"
                     /locus_tag="ACHKCL_000211"
     CDS             41060..41467
                     /gene="ygfX"
                     /locus_tag="ACHKCL_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5761257.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41507..42028)
                     /gene="fldB"
                     /locus_tag="ACHKCL_000212"
     CDS             complement(41507..42028)
                     /gene="fldB"
                     /locus_tag="ACHKCL_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5761258.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42140..43036
                     /gene="xerD"
                     /locus_tag="ACHKCL_000213"
     CDS             42140..43036
                     /gene="xerD"
                     /locus_tag="ACHKCL_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5761259.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTLWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43061..43771
                     /gene="dsbC"
                     /locus_tag="ACHKCL_000214"
     CDS             43061..43771
                     /gene="dsbC"
                     /locus_tag="ACHKCL_000214"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5761260.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43777..45510
                     /gene="recJ"
                     /locus_tag="ACHKCL_000215"
     CDS             43777..45510
                     /gene="recJ"
                     /locus_tag="ACHKCL_000215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5761261.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45601..46699
                     /gene="prfB"
                     /locus_tag="ACHKCL_000216"
     CDS             join(45601..45675,45677..46699)
                     /gene="prfB"
                     /locus_tag="ACHKCL_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5761262.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46709..48226
                     /gene="lysS"
                     /locus_tag="ACHKCL_000217"
     CDS             46709..48226
                     /gene="lysS"
                     /locus_tag="ACHKCL_000217"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5761263.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48269..48817)
                     /gene="idi"
                     /locus_tag="ACHKCL_000218"
     CDS             complement(48269..48817)
                     /gene="idi"
                     /locus_tag="ACHKCL_000218"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5761264.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            48872..48943
                     /locus_tag="ACHKCL_000219"
     CDS             48872..48943
                     /locus_tag="ACHKCL_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761265.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(48940..49065)
                     /gene="yqfG"
                     /locus_tag="ACHKCL_000220"
     CDS             complement(48940..49065)
                     /gene="yqfG"
                     /locus_tag="ACHKCL_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5761266.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49067..50515)
                     /gene="uacT"
                     /locus_tag="ACHKCL_000221"
     CDS             complement(49067..50515)
                     /gene="uacT"
                     /locus_tag="ACHKCL_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5761267.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50951..52870
                     /gene="ygfT"
                     /locus_tag="ACHKCL_000222"
     CDS             50951..52870
                     /gene="ygfT"
                     /locus_tag="ACHKCL_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5761268.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52870..53358
                     /gene="ygfS"
                     /locus_tag="ACHKCL_000223"
                     /pseudo
     CDS             52870..53358
                     /gene="ygfS"
                     /locus_tag="ACHKCL_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(53394..54761)
                     /gene="ghxQ"
                     /locus_tag="ACHKCL_000224"
                     /gene_synonym="ygfQ"
     CDS             complement(53394..54761)
                     /gene="ghxQ"
                     /locus_tag="ACHKCL_000224"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5761269.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54797..56113)
                     /gene="guaD"
                     /locus_tag="ACHKCL_000225"
     CDS             complement(54797..56113)
                     /gene="guaD"
                     /locus_tag="ACHKCL_000225"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5761270.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(56131..57531)
                     /gene="xanQ"
                     /locus_tag="ACHKCL_000226"
     CDS             complement(56131..57531)
                     /gene="xanQ"
                     /locus_tag="ACHKCL_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5761271.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(57696..60566)
                     /gene="xdhD"
                     /locus_tag="ACHKCL_000227"
     CDS             complement(57696..60566)
                     /gene="xdhD"
                     /locus_tag="ACHKCL_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5761272.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(60563..61342)
                     /gene="ygfM"
                     /locus_tag="ACHKCL_000228"
     CDS             complement(60563..61342)
                     /gene="ygfM"
                     /locus_tag="ACHKCL_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5761273.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(61392..62720)
                     /gene="ssnA"
                     /locus_tag="ACHKCL_000229"
     CDS             complement(61392..62720)
                     /gene="ssnA"
                     /locus_tag="ACHKCL_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5761274.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(62723..65821)
                     /gene="ygfK"
                     /locus_tag="ACHKCL_000230"
     CDS             complement(62723..65821)
                     /gene="ygfK"
                     /locus_tag="ACHKCL_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5761275.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        16002 a        16564 c        17565 g        15922 t
ORIGIN      
        1 ggtttccctg ctcaattgct ttcattaaac cgcacgagta ttttattttt accgccggac
       61 ggtaccctaa aaataaaata aggataattt atgcggctta gtgactattt tccagaatca
      121 tcaatctcag ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta
      181 tcattgctgg ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc
      241 attaacaatg gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg
      301 gaatgtgggg cgcttaaaac cgggatgtct ttgacattac ttgaacaagg tgtttatttt
      361 ccggggaatg acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg
      421 cacattggcg ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa
      481 ctcttaacag catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc
      541 tctcctctac aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt
      601 gggggctttt tgactgcaat ggtcatcccc aatattggtg cttttattgc ctggggtttt
      661 attactgcgt tatttattcc caccggttgg ctgcctaacg aacatttcgc caaaattgtc
      721 ggcccgatga ttacctattt attgcccgtg atgattggtt ctaccggtgg tcatctggtc
      781 ggcggtaaac gcggcgcggt catgggcgga ataggtacta ttggtgtgat cgtcggcgca
      841 gagatcccga tgttccttgg ctcaatgatt atggggccgc tcggtggtct ggtcataaaa
      901 tatgttgata aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac
      961 ttctcattag gtatcgcggg gatgctcctt tgcctgctgg gttttgaagt gatcggcccg
     1021 gcggtgttaa ttgccaatac tttcgtcaaa gagtgtattg aggcgctggt acatgcgggt
     1081 tatctgcctc tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg
     1141 atcgatcagg gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc
     1201 atcttcttta tggtggcctc taacccaggt ccgggcctgg ggctgctgct ggcgtttacc
     1261 ttgtttggta aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc
     1321 ggtgggatcc acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc
     1381 atgattgcgg gcggtatgtc tggcacctgg atgtttaact tactggacgg tggtctggtg
     1441 gctggcccaa gtccggggtc tatctttgct tacctggcgc tgacgccgaa aggttcgttc
     1501 ctggcgacaa ttgccggtgt tacggtaggt accctggtgt cctttgctat cacttcgctg
     1561 atactgaaga tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc
     1621 aatgcggtta aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc
     1681 gcctttgttt gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa
     1741 cgcctggaaa aagcggggct ggcaattgaa gtaaaacatt acgccataga aaacgtgcct
     1801 gcggatgcgg atatcgtcgt tactcatgcc agtctggaag ggcgcgtgaa acgtgtgacg
     1861 gataaaccac tgatattgat taataactat attggcgatc caaaactcga cactttattt
     1921 aatcaattaa ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg
     1981 ctgctattta tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg
     2041 ataatgaatt actggtgagt gtgatttctg acagcgtctg tttatccacc tggaaagcgg
     2101 cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta
     2161 ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata
     2221 aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcgg
     2281 gctacagcta cgaatatttt ggcggcaatg ccacttatat gattattccc gaaatcgcca
     2341 ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag
     2401 agcctatgtg ctgcattatt ggcgcttatc atgccaatta tcacaccacg caatatgttt
     2461 atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcaggtc
     2521 cgatgggcat tggcgctatc gattacgcca ttaacggcgg catacaaccg tcgcgggtgg
     2581 tggtggtcga tatcgacgac aaacgtctgg cgcaggtaca gaagctgctg ccggtggaac
     2641 tggcggccag taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatcctg
     2701 tccagatgct gcgggcgctg acaggagatg ccgggttcga tgacattttt gtttatgcgg
     2761 cggtgtctgc tgtcgttgag atggctgatg aattactggc ggaagatggc tgtctgaact
     2821 tctttgccgg gccgacggat aaaaacttca aagtgccgtt taatttctac aacgttcatt
     2881 acaacagcac gcacgtcgtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga
     2941 ttgcccttag cgccacaggg cagttacagc cctcgtttat ggtgacccat atcggtggcc
     3001 tggatgcggt gccagaaacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca
     3061 tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa aaaggcaaaa
     3121 ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc atctggaatg
     3181 aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag gccgcgcaat
     3241 gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc
     3301 gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca
     3361 ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca
     3421 tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat
     3481 cgcggttgat cccattgaag gcacgcggat ggtggcgatg gggcagagca atgcgctggc
     3541 ggtcatggct ttcgcccctc gagatagcct gttacatgcg cccgatatgt atatgaaaaa
     3601 gctggttgtt aatcgactgg ctgccggggc gattgatctg tctttgcctc tggcggacaa
     3661 cttgcgtgac gtggcgcggg cgttaggtaa accgctggac aagctgcgca tggttacgct
     3721 ggataaaccg cgtctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt
     3781 tgccctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta
     3841 cgatgtgatg tacaccatcg gcggtgcgcc ggaaggcgtg atttctgcct gtgccgttaa
     3901 agcattaggc gggaatatgc aggccgagct gattgacttt tgtcaggcca aaggggatta
     3961 cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgcaaggcaa tgggtgttga
     4021 cgttaaccgt gtgtactcgc tcgatgtact ggtaaggggg aacgatatcc tctttagcgc
     4081 tacgggcgtc acgggcggcg agttggtgaa cggtatccaa cagacggcga atggagtgcg
     4141 gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgta
     4201 ttgatggtga tctatcggaa gaccaatggc gacgctgtca gaagatgatg tgcttgagca
     4261 actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca
     4321 ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga gcgtcgaata
     4381 tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcactgcg
     4441 tttgatttat gcgctgggga aaatggataa atggctgtac gccgatatca cgcatttttc
     4501 ccagtactgg cattacctga aagaacagga tgaaacgctc ggatttgccg atgacatgac
     4561 ttgggatttt atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt
     4621 gaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt
     4681 caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc cgtgaaaatt
     4741 gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga gatagaacgc
     4801 gtgcataaac cgttgctgcg tatgctggcg gccttacaga cggtaaatcc gcagcgccga
     4861 accgtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccctaac caccttctgg
     4921 gcatatctcg cgcaacaaga cccggagctg cccgctattc agacgctccc gatggatggt
     4981 tttcaccatt acaatagctg gctggatgcg catcaattgc gccccttcaa aggcgcacca
     5041 gagacatttg acgttgcgaa actggcggaa aatctgcgcc aggtcgtgga aggggattgt
     5101 acgtggccgc agtacgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc
     5161 gcaccaataa tcatcgtcga aggaaactgg ctattgctgg atgacgataa ctggcgccag
     5221 ctagcgcaat tttgtgattt ctcgatcttt atcaatgcgc cagctactgc gttgcgggaa
     5281 cgactggtgg ggcgcaagct ggcgggaggg ttatcgctgg cagacgcaga agctttttat
     5341 gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg
     5401 accttaatga tgaccgctac gggtgaatat cgtctggtgg attaatgttc ctgattagac
     5461 catcttccag tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga
     5521 ttgctacgac gctcgtgcct cgctggcggc acttctccag ccagctttca aacacgctca
     5581 accaattttc gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg
     5641 cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg
     5701 cgctggcagt gtctgctaac tgacaacatt gcagggcagc atgggtacgt tgagtgattt
     5761 catccgccgg gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg acggtgctgt
     5821 gaaaaagttg gttttccgct tcctgaaaca gtacgccaat ctttgccgcg cgctggcggt
     5881 ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt accggctgtg ggcgtaagaa
     5941 gacctgccat tactcgtaac agcgtcgatt tccctgcgcc attgtcgccg gtcaacgcca
     6001 accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag
     6061 gccagcgata agatatctga tttaacgtta acatagtggc tggagcgctc cgttttgcaa
     6121 ttgccacgct tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa aaagaataat
     6181 cagcgaacca cgttcaaacg cccagtgttg taagcgctgc aacagcattt cgctggcctg
     6241 cggcgtcagg cggctaaagg cttcatcaag aattaatagc tttggctgca tcgcgatggc
     6301 gcaggcgatg accacgcgct gtgtctctcc accggagagt gttgccgggt gacgatggcg
     6361 caacggctgg cactcggtca gagtcagggc ggcatcaatc cgcgccataa tctctgcttc
     6421 agcaagacac agattttctg gcccaaaggc aacttcttct tccacgctaa aggtacagcc
     6481 ggagagctgc aaatagggcg attgctggac gagctggatg gtggcggatt gttcattaag
     6541 cgggagatgc ccaatgggcg taccgaggag cgtccccgtg cctgttatct ctcctggcag
     6601 aaagtccgga taccagcccg ccatcagctg tgccagcgta cttttgccac tgccgttgtc
     6661 accaaagatg gcgaccatcc ccggtttgac atagtgaaag tcataacaga acggaggatg
     6721 cgttgagtgt gtcgggcaat aacggaactg ttctaacgta accatatcca cgctccgaat
     6781 tctgtcagca tcagcaagat catggtgtaa cgcgctacgc gttgtagggt gctatcggct
     6841 ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg cccgcatatc cagcgccgca
     6901 ccacggacag caagatcgtt tagcgcattg tgtgttagcg gaatagttaa cgcgggcatc
     6961 gcacgtaaac gctgatacca gccctcatcc agcggcacgc cgcgggcacg ctgcgcttca
     7021 tgaataatcg ctaattgccg ttttaactgc tcaacgacca ataacggccc ggcaaacaga
     7081 taagcgacgc ccggcggcag gcgagaagca aacagagcgc ggataaaaca ttgcacgggg
     7141 acgtactgca tccacaactg tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta
     7201 atagcatgcg tccaacgctc cggcgaacgc ggcgtgccgc ttagccattc tgttagccag
     7261 ccaccgtgta ccagccacag cccggctccc agcgagaaca tcagccagac gacatatttt
     7321 gcccgccgcc gcgtggcctt tagcgcaata aggcaaaaaa aggtcgccgc gctatagatc
     7381 ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg ccgccaatgc ccatagtgtt
     7441 aatgaggtaa atggatgcat ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg
     7501 cgaatcggca gctggcgcag taaaagccag acaatcaccg ctgtaagagt tttatcgacc
     7561 aggttcgcgc ctatcaccgt aatcgccacc gactccacca ggttttgccc catcgagtgc
     7621 atccaggcaa cgaacaaatc ggctccgcta ccggtgacac cgccgaacag cgcagtacgc
     7681 agcggtacag cgacgaccgt taccgcaagc gtaataatca cgccgctgac aacgactttt
     7741 ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg cgaccaggcc gatgaccatc
     7801 gcgacggggg caaaagcggc agcgatcggg tcggtcagta atccccagag taaattggtc
     7861 agtaagccgg tcagcatccc gatcaccgga ccaagcagta ccgcgctaat aagtgtgccg
     7921 atggagtcga gaaatatagg taatttgacc atgctggcca gttgcccgcc gatcatattg
     7981 atggcgatag atataacgat caacaccagt gcctgactgg aaaagtggct acgcgccata
     8041 agaaaacctc tctgaaagta gttatttaac gggggaaata tgcaactcgt cgtaaccaga
     8101 aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg ctaccgatca ccagttcaag
     8161 cgtggtccgc acggtacagc cgggcatcat atgaccgcca agcatcgtgc cgtgtggatc
     8221 ggaaacgcag agatgaagat gctcaccact ctgttccaac gtgccgttta gtgagatcac
     8281 ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca aggcgatgtc
     8341 agtcaggcta ccggtacagc ctgcaatcca ggcggcatga agttgctgct gctgtgcaaa
     8401 agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg cgcagtgcgt aaaagcgtgc
     8461 ggaggaagaa tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt
     8521 acggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca
     8581 caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg
     8641 ggcggggctg aaaacgtgac gtaagtcaga taattgtttt ctttgactgg acaatcattc
     8701 cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca
     8761 attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta
     8821 ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag
     8881 cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatccggac
     8941 gccgggcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc
     9001 atttgttgaa atacgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg
     9061 atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc
     9121 tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc
     9181 gcgagcatgg cgaagcgcac attgccgccg gggctaaaaa agtgctcttt tcacatcctg
     9241 gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg
     9301 agcaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat
     9361 tgttagatga tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc
     9421 acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc
     9481 agtcgatcat tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat
     9541 ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg
     9601 atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa
     9661 aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg
     9721 tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg
     9781 gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa
     9841 tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct
     9901 gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg
     9961 gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg
    10021 aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa
    10081 caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac
    10141 gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt
    10201 cgtctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg
    10261 gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaatcctgtc caaaaaatac
    10321 gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct
    10381 tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct
    10441 gaactggacg cgctgggtaa ggcactgaaa gaacctgctc gcccgatggt ggctatcgtt
    10501 ggtggttcta aagtatctac caaactgacc gttctggact ccctgtctaa aatcgctgac
    10561 cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg
    10621 ggtaaatccc tgtacgaagc tgacctggtt gacgaagcaa aacgtctgct gaccacctgc
    10681 aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac cgctccggct
    10741 accctgaaat ctgttaacga cgtgaaagct gacgagcaga tcctggatat cggtgatgct
    10801 tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg
    10861 gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc
    10921 gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg
    10981 ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc
    11041 gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa
    11101 atcacagggc agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca
    11161 tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    11221 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga
    11281 gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc
    11341 tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt
    11401 cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaactgc
    11461 tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc
    11521 tggtaaaggc gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg
    11581 tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga
    11641 ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca
    11701 cttcgcagct accggtaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc
    11761 tctgcaagag aacatcgaaa tctgctctaa atacctggag cgcatgtcca aaatcggcat
    11821 gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca
    11881 catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgaact
    11941 gagcaaaatc agcccgcgtt tcaccatcgc agcgtccttc ggtaacgtac acggtgttta
    12001 caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc
    12061 taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc
    12121 tactgctcag gaaatcaaag actccgtaag ctacggcgta gtgaaaatga acatcgatac
    12181 cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta
    12241 tctgcagggt cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga
    12301 tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt
    12361 ccaggaactg aacgcgatcg acgttctgta agtcattcct ttctgcttat ctcaaggccc
    12421 gctctgcggg tctttttttc gccaaaagca aaattgcctg atgcgctatg cttatcaggc
    12481 ctacacgtat cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca
    12541 cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga
    12601 tttttttgcc aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta
    12661 tgctttattg tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca
    12721 ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa
    12781 cggcgcggga agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat
    12841 cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc
    12901 ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc
    12961 attagtccgt tacggtatta tcgcctttac gctaatcgca gccctgggac gcgtgggtgt
    13021 acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt
    13081 gcaggggtca ctttctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg
    13141 tgccggagaa tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt
    13201 ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat
    13261 tgccggaaat attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg
    13321 cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc
    13381 tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc
    13441 gtcgattaat ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg
    13501 ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc
    13561 gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag
    13621 gccagataat actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat
    13681 caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat
    13741 atccaacgtg ttttcttatt actttcaagg tcttgctctt ggggcagcta tgattctgcc
    13801 gctcggccca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat
    13861 gattgcctta ctttgcgcta tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg
    13921 tagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagt
    13981 cttcttgctg tggtatggtt ttggcgcttt taaaacagcg atgagtagta atattgagtt
    14041 agctagtgcc gaagtcctga agcaaggcag atggaaaatt atcgccacca tgttggcagt
    14101 gacctggctg aatccgcatg tttacctaga tacttttgtt gtactgggca gccttggcgg
    14161 gcagcttgac gtcgaaccaa aacgttggtt tgcgctcggg acaattagcg cctctttcct
    14221 gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa
    14281 atcacagcgc attatcaatc tggtagtggg atgtgtcatg tggtttatag ccttgcagct
    14341 ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa
    14401 aataaaacag aggcgctaag cttgcctcca gagatcctga attttgggca agtcaattaa
    14461 cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc
    14521 gggatggcag cgcaggctaa cgaattgccg gatggaccgc atattgtcac ctccggtacg
    14581 gcaagtgtgg atgcggtgcc agacattgcc actcttgcga ttgaagttaa cgtggccgcg
    14641 aaggatgccg ctacagccaa gaaacaggca gatgagcgcg tcgcacaata catttccttc
    14701 cttgaactca atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca
    14761 gattatgatt atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg
    14821 gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg
    14881 ggtcttaacg aaattcgttc agtgtcgctg ggcgtggcgc agccggatgc ctataaagac
    14941 aaagcgcgta aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc
    15001 tttcatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc
    15061 agcccaatgg tgcggatgat gaaagccgat gccgcgccgg tgtctgccca ggaaacttac
    15121 gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg
    15181 gatcaacaac ccgctaaaac acctgcagca caataatact tgtagcccga cgtattcgtc
    15241 gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac
    15301 gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg
    15361 gacaaggatg cggggagggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat
    15421 tttattagcg caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc
    15481 gccatttggt aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg
    15541 caaagataat aatatcagcc tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc
    15601 ggtttatcaa cgactttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg
    15661 agaaattgtg attggcatag ataatactta tccgacaatt attttcgatc agttgattag
    15721 tctcggagat aagtatgaag gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt
    15781 tatcgataat ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc
    15841 acgagttcag gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgaactt
    15901 tctcgtttct cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgcccta
    15961 gctacaaatg cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg
    16021 accttgtggg atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag
    16081 cgccgtggag cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa
    16141 tccggcaacg ctgaaatatt ttgatgcgcc gcacaggccg atgtatatgc aggaatatct
    16201 ttattcaatc agaaatcatc gttataccgc cacgatgctt cagcatattg ctgaagatcg
    16261 tgacgggaca aaccattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc
    16321 gaagacgtgg ctaagatcgt ggtgaatatg tccgccgggc gcattttcca gatagcgatg
    16381 cagataatcc cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag
    16441 cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg
    16501 ttcgttgtga tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc
    16561 aatcgacgga gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc
    16621 gatgccgttc atcagatcga ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc
    16681 aaactccagg ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt
    16741 ggaaatctcc tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat
    16801 cttgcgcagg gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc
    16861 ggtttccagt aaatgcacca ccgattcctg tagcacttcc gaatacatca taaacggagg
    16921 aatttccggg ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact
    16981 ttgcagcggc agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt
    17041 gaccacgtta tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc
    17101 gtcgggcaag ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg
    17161 ggtaccgacg cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc
    17221 aggaatcaca atatccgcca gttctgcaac gcgcggatcg tgatagtgat tgagttcaat
    17281 gatcactttc ttcgctcgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca
    17341 gactcgacca tctggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa
    17401 gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc
    17461 gccctgattg atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac
    17521 agcatcggcg tcagaaagta catcgtcagc ggcggcgctg attgacgccc ccgtcagcag
    17581 gcgaatttga tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt
    17641 gggtagggct ttcggcgaac ccgctggggt aaagccgcta aatgccacca tgtcgttatg
    17701 ctggataatt tctgccgctt catcggcggt catccttgtc cactgagttt ccatttttac
    17761 tccttcgttc gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg
    17821 ttcatccctt cctggtaatc ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt
    17881 tcaaattcat cggagttcat ggtgtgtgct tcgcccagta cacgcagctc ttctttgata
    17941 acggcaatgg ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct
    18001 tccagttctt ccacttccac aacatggttg aggatgccga cagccagcgc gcgctgggcg
    18061 gtgatgggcg aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc
    18121 aggttgtgaa tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag
    18181 gttgaggtac tggcggcgat aatcagatcg gaactcatga tcatttcaaa tgcgccaccc
    18241 caaacactac cttccaccat cgaaataatc ggtttcggga atttttggat catgcgggtg
    18301 atttgacgca atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga
    18361 atatcgtgac ctgcggagaa gactttggat ccactcggcg cgcgcaaaat gatacagcga
    18421 atttccggcc ggttgagatc gcttaacgcc tgcataagat catcaataaa gactttactt
    18481 aaggcattaa gttttcggcc atagttaaac tcaatgaccg ccactttgtt gatagtgaca
    18541 acgttaacat actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa
    18601 ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag
    18661 cgtctggcgg taatagcgat cgaaatcttc attcgcgaac aggtgattca gtacttcttc
    18721 ttcggtctgc ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc
    18781 actggcagtt agcgcggttt tgaagtcgat gatggcgtgc cagatctcat cgattccacg
    18841 tttttccagt gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag
    18901 aatatgcagg gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc
    18961 atcgtctttg ttgataacga tcagatcagc cacttccatc agcccttttt taatgccctg
    19021 cagatcatcg ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac
    19081 ttctgtttcc gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgctgc
    19141 ttcgcataac agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga
    19201 tggtaccggg cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc
    19261 cccgagaatg ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt
    19321 taatccctct cgaatcaaca acatgccaaa ggcctcaaga aaggtacttt tccccgcgcc
    19381 gggggtgccg gtaacgccca gtcgcagggt gttaccgcag tacggcataa tggcatcaag
    19441 cagctgcgta cttagtgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg
    19501 ggcgagtgtg gcatgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc
    19561 attaatcatg atgctggctt atcagattca gtacgtcgcg cacactgtcg agcataggtg
    19621 tacctggacc ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggtg
    19681 gaatgacgcc accggcgact acgcagatat cttcgcgtcc ccattttttc agcgcttcga
    19741 ccagttccgg gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa
    19801 cgtcattttc tactgccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat
    19861 ctacgtcgaa accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgtc
    19921 catccaggcc cattttggcg atcagaatgc gcgggcgacg accattgtcg gcaaggaact
    19981 gctccgtttg cgcaacaatg gcatcgaact cggaggccga tttctcagat tgatgatagc
    20041 tttgcgcaat cacgccggta acacactggc ttggcaccag ataacggtcg aaagcggctt
    20101 ccagcgcatc ggaaatttca cccagggtgg cgcgaacgcg agcggcatta acagcggcag
    20161 ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg
    20221 cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca
    20281 tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg atccagtttg tacttgttga
    20341 caccaacgat gacacgcttg ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt
    20401 cgatcattcg ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga
    20461 tctgttggat aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat
    20521 aggatccggc cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct
    20581 gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg
    20641 cgttggtatg cagtgactga gtaccgccca gcgtcgcagc cagcgcttca atggtggtgc
    20701 ggataacgtt gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg
    20761 tacgcagcgc cagtgatttc gggtcctgtg cgccaaatcc actgaccgct tcgctccata
    20821 aataacgtgc cgcacgcaac atggcgacgt tcataaacag atccatgccg atgccgaaga
    20881 agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga
    20941 tgtactcaat cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt
    21001 cacccatgtg gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc
    21061 aggcgatgat gtcggcgata atgcgcattg acggttttgg cgggtaaata taggtgttgc
    21121 ggcagaggta ctctttgaga atatcgtttt gaatggtgcc ggtcagttta tcaggtgtaa
    21181 caccttgctc ttccgcggcg atgatataaa acgccagtac tggtagcact gcgccattca
    21241 tggtcatcga aaccgacatt ttatccagcg ggatctggtc gaacaggact ttcatatctt
    21301 ccacggtgtc gatagcgacg cccgttttgc cgacgtcacc cgccacgcgt gggttatcgg
    21361 agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg
    21421 ccaggttacg gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac
    21481 ggatggtcca cggttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca
    21541 aaccaggaag ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct
    21601 tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc
    21661 tcaattcctt gttggcaagc tgttgccact cctgctcgtt agacattatt tgctccgtaa
    21721 aacgcaatca aaatgagggt atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc
    21781 agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat
    21841 gtgattcaag gaatacaaaa agaagtgagg ataacgcctg atatgtgcta tcaggcatta
    21901 tttgatggat taatcctgac gaaggacttt gtgaccataa tcgagcaacg catcagtgac
    21961 tttacgcatc atgcggcttt caggagcaaa gcggtgccag tagagcatcc ggcgttgaaa
    22021 tagccctggc gttaagtcaa tcagttcacc gctggctagt tctttctcga tttgcaggtg
    22081 cgggatcata cagcaggtgg tgccctggcg agcaagttgc acgaacgctt ctgaagaatt
    22141 aacgatatgg cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg
    22201 gtgcatatcg tcaagatggt caaacgcgac cactggcgct ttcagtaatg ccgaacgcgt
    22261 tacgccgtta gggaaatatt tttcggcaaa gggttttgag ctgacgaaca gatagtcgag
    22321 cgcaccaagt ttatcgacaa gacaactcgg cagcgcctga tgttgaatac tcaccgcgcc
    22381 gaccacttcg ccgcgacgca gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt
    22441 gaggcggata ggcgaatcag ccaatacagg agccagtgcc ggaagcaacc acgtcgccag
    22501 actgtcggca ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc
    22561 cagccactct tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg
    22621 ttccgtcggg cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag
    22681 ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc
    22741 aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt
    22801 cattgctgtt gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc
    22861 aggttgcata taccgcagcc gtcggcgggt aacatttgta tcaatggtta aacgcgcagc
    22921 ttaaaagatc agggatttgc gctattgcat ttcgcttagc aacttcgcgc cctggttatg
    22981 ttgaatggcg tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg
    23041 tatgctttat ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag
    23101 ttaaccacgc ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa
    23161 atttcatacc acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg
    23221 ggcggcactt cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc
    23281 cgcacacttt attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag
    23341 ttcagatgct tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga
    23401 agtcgacagc cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat
    23461 gatcaaaggc ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa
    23521 atttatctgt attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgcc
    23581 agtagaagtt atcccgatgg cacgtagtgc agtggcgcgc cagctggtga aactgggcgg
    23641 tcgtccggaa taccgtcagg gcgtagtgac cgataacggc aacgtgatcc tcgacgtcca
    23701 cggcatggaa atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt
    23761 ggtgactgtt ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga
    23821 cggtgtcaaa accattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct
    23881 cttcattaaa tttggtgaca tgtgtcacgt ttttacctgg atattgtcga ttgctctaaa
    23941 taaatcctct aaaccagcat attcaaccaa gaaatacctt tgcgtgatat ttcctcaaca
    24001 tcgcgacgca aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggatg
    24061 caaatccgca cacaacattt caaagacagg attgggtaaa tggcaaaggt atcgctggag
    24121 aaagacaaga ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt
    24181 cgtgcagctg gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta
    24241 aaagaatcca tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa
    24301 gacgtgatca acgccgcaga aaagctggtc gctattggct gtttctgtat aggaacaaac
    24361 caggttgatc tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca
    24421 aatacgcgct ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg
    24481 ccggaagcca atgctaaagc gcaccgtggc gtgtggaaca aactggcggc gggttctttt
    24541 gaagcgcgcg gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc
    24601 attctggctg aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg
    24661 ctgggcaacg ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg
    24721 agtctgcatg taccagagaa tccgtccacc aaaaatatga tgggcgcgaa agagatttcg
    24781 ctaatgaagc ccggctcgct gctgattaat gcctcgcgcg gtactgtggt ggatattccg
    24841 gcgctgtgtg atgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg
    24901 acggaaccgg cgaccaatag cgatccattt acctctccac tgtgtgaatt cgacaacgtc
    24961 cttctgacgc cacacattgg cggttcgact caggaagcgc aggagaatat cggcctggaa
    25021 gttgcgggta aattgatcaa gtattctgac aatggctcaa cgctctctgc ggtgaacttc
    25081 ccggaagtct cgctgccact gcatggtggt cgtcgtctga tgcacatcca cgaaaaccgt
    25141 ccgggcgtgc taactgcgct gaacaaaatc ttcgccgagc agggcgtcaa catcgccgcg
    25201 caatatctac aaacttccgc ccagatgggt tatgtagtta ttgatattga agctgacgaa
    25261 gacgttgccg aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt
    25321 ctgctgtact aattcccctt ctctgaaaat taacgggcag gtcactgact tgcccgtttt
    25381 tttatccctt ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga
    25441 aaataaatca aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcggg
    25501 acagcatggg gctgtaacgg tgaagccctc accgaagcga gggcttaaag gagaagggtt
    25561 atgatgcgac ttgtcatcat actgattgta ctgttactga taagtttcgg agcttattaa
    25621 cagtcagtct caggggagga gtcatcctcc cttaccctta ctcactaaat taggtcaaag
    25681 aatcaacgat gtcaatcagg gcgatgcggg gtgcatcgcc ctgaaccgaa ctttttgata
    25741 gccacttcat cgcccacgtt tactgccgta attaatccct tattcctgca atgtatgtct
    25801 gccttacatg acgttttacg agtgcgctcg aagaaaagat ttaaaggtag atcaatgcgt
    25861 tgtggttatt taaagagagt ttacgatggc agggcagcat ggggctgtaa cggtaaagcc
    25921 ctcaccgaag cgagggcttg aaggagaagg gttatgatgc gacttgtcat catactgatt
    25981 gtactgttac tgataagttt gccagcttat taacagtcag tctcagggga ggagtcatcc
    26041 tcccttaccc ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc
    26101 aggtgtatcg cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga
    26161 tatcccactc ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg
    26221 gttgcgtttt atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc
    26281 ccaggcgctg accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg
    26341 aaagggggag cacgtcacgc acatccagtt ttggctcatg gattttcaac ctgttcatca
    26401 ccagttcgct ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca
    26461 aaactggaag atatacgcgc ttaccggcgc gccagagttg ttctataagc ggctgggtgt
    26521 cgagttcacc atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat
    26581 aagtcatcat ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg
    26641 cacgacgacg ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt
    26701 ctggtagttg cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag
    26761 gctgtaaccc ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc
    26821 ggacggaccg agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg
    26881 gctcagggga ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct
    26941 accacagtaa accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc
    27001 ttgttcaagt aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact
    27061 tgccgcgtag tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc
    27121 ggcaatgaag accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa
    27181 ccgttggttc agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt
    27241 cacacgcagt gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc
    27301 ctgctgattg actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta
    27361 tagctaccct gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag
    27421 catatcatga atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc
    27481 ctggttacaa cgaaatgaac cagtatctga accaacaagg gacgggtctg acccctgctg
    27541 agatgcatgg tttaatcagt gggatgatat gtggcggtaa cgatgacagc tcatggctac
    27601 cgctacttca cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcat
    27661 tgcgtaaaat gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc
    27721 tttatctgcc tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt
    27781 gggtcaatca cttcctgctt ggtcttggtg ttacgcaacc gaagctggat aaagtgaccg
    27841 gcgaaaccgg tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag
    27901 acgaagatca ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg
    27961 ccgcgctgtt atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa
    28021 aaccgactct acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg
    28081 caagagtttc agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg
    28141 ctgatttttg ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag
    28201 aacagtgact tctggtactt caccggcttt aacgaaccgg aagcggtgtt ggtgctgatt
    28261 aaaagcgatg acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg
    28321 gagatctggt ttggccgtcg cttaggccag gatgccgcgc cagagaaact gggcgttgac
    28381 cgcgcactgg cattcagcga aattaatcag caactttatc aactacttaa tggtctggat
    28441 gtggtttacc atgctcaggg cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg
    28501 gaaaaactgc gtaaaggttc gcggcaaaat ctcaccgcac cggcaacgat gatcgactgg
    28561 cgtcctgttg ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtactccgc
    28621 cgcgcgggag aaatcaccgc catggcacat acacgggcga tggaaaaatg ccgtccggga
    28681 atgttcgagt accatctgga aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgc
    28741 tatccgtcct ataacaccat tgtaggcagc ggtgaaaacg gctgcatttt acactacacc
    28801 gaaaacgagt gtgaaatgcg cgacggcgac ctggtgttga ttgacgcagg ctgcgaatac
    28861 aaaggttacg caggcgatat cacccgtacc ttcccggtca acggcaaatt cacccaggcc
    28921 cagcgcgaaa tctacgacat tgtgctggag tctcttgaaa ccagcctgcg cctgtatcgt
    28981 ccgggaacct ccattctgga agtcaccggt gaagtggtgc gcattatggt tagcggcctg
    29041 gtaaaactcg gcatcctgaa aggtgatatt gatgaactga tcgctcagaa cgcccatcgt
    29101 cctttcttta tgcatggcct tagccactgg ttaggactgg atgtccatga cgttggcgtt
    29161 tatggtcagg atcgctcgcg cattctggaa ccggggatgg tactgaccgt agagcctggt
    29221 ctgtatatcg cgccggatgc ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt
    29281 gaagacgaca ttgtgattac cgaaaccggc aacgaaaacc tcaccgccag cgtggtgaaa
    29341 aagccggaag aaatcgaagc gttgatggct gctgcgagaa agcaatgagc gtaatcatcg
    29401 tcggtggcgg catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacgggg
    29461 cgctgccggt acatttgatt gaagcgactg cgccagagtc acatgctcat ccgggctttg
    29521 atggacgagc gatagcgctg gcggcgggta cctgtcagca actggcgcgc atcggcgttt
    29581 ggcaatctct ggcggattgc gcaactgcca tcaccaccgt gcatgtcagc gatcgaggtc
    29641 acgctggatt tgtcaccctc gccgcagaag attaccaact ggcggcgctg ggacaggttg
    29701 tcgaattgca caatgtcggg caacggctgt ttgcattgct gcgtaaagca cctggcgtaa
    29761 cgctgcattg ccctgatcgc gtggctaacg ttgcccgtac tcagagtcac gttgaagtga
    29821 cgctggaggg tggcgagacg ctgacgggcc gcgtgctggt agccgctgat ggcacccatt
    29881 cagcgttagc cactgtgtgc ggcgttgact ggcagcagga gccttacgaa caactggctg
    29941 tgattgccaa cgttgcgact tccgttgcgc atgaagggcg cgcttttgaa cgctttacgc
    30001 aacatggccc gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc
    30061 atccactgga acggcgcgaa gaggtgctgt cgtggagcga cgagaagttt tgccgtgaac
    30121 tccagtcggc ctttggctgg cgacttggga aaattaccca cgctggtaaa cgcagtgctt
    30181 atccgctggc gttaacccac gccgccagat ctattaccca tcgtaccgtg ctggtgggca
    30241 atgcggcgca aaccctgcac ccgattgccg ggcaagggtt caacctcggt atgcgagatg
    30301 tcatgagtct tgcggaaacc ctgactcagg cgcaggagcg cggagaagac atgggtgatt
    30361 acggcgtatt gtgtcgttat cagcagcgtc gacagagcga tcgcgaagca accattggcg
    30421 tcacggacag ccttgtacat ctttttgcca accgttggac gccactggtt gtcgggcgca
    30481 acatcgggct gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca
    30541 ccctcggttg ggtggcgcgt tgaaggcggt tattaaggaa taaacaatgc aaagtgttga
    30601 tgtagccatt gttggcggcg gcatggtggg gctggcggtt gcctgcggct tacagggtag
    30661 cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg cggatgcacc
    30721 accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg
    30781 cgtctggcag gacattctct ctcgccgagc cagctgttat cacggtatgg aagtgtggga
    30841 caaagacagt tttggtcaca tctcgtttga cgatcaaagc atgggctata gccatcttgg
    30901 gcacatcgtt gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc
    30961 agatatcact cttttggccc ccgcagaatt acagcaggtc gcctggggag aaaatgaaac
    31021 cttcctgacg ctgaaagatg gcagtatgtt aactgcgcgt ctggtgattg gcgcggacgg
    31081 cgctaattcc tggttgcgca acaaagccga tattccgctg actttctggg attatcagca
    31141 tcacgcgctg gtagcgacca tacgcacgga agaaccgcat gatgcggtgg cgcggcaggt
    31201 tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat
    31261 tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg aagacgaatt
    31321 taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc
    31381 gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag tttgccgcgc accgtctggc
    31441 gctggtgggc gacgccgcgc ataccattca cccgctggcg gggcaggggg taaatcttgg
    31501 ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc agggtaaaga
    31561 catcgggcag tacatttatc tgcgtcgcta tgagcgtagc cgcaagcaca gtgcggcgct
    31621 gatgctggct ggtatgcagg gattccgcga tctgttttct ggtaccaatc cggtgaaaaa
    31681 actgctgcgt gatattggtc tgaaactggc cgacacgctt cctggcgtta aaccacaact
    31741 tatccgccag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctctt
    31801 ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt
    31861 cctcatttga aataaactaa tttcacctcc gttttcgcat tatattttct aatgtcacga
    31921 atttttgatt tagtgttttt tgacattttt tcagctctta atattgcctt attcaaatta
    31981 actttctcat cacatcatct ttatatagaa acttgtgtat tttttggttt tttattctgt
    32041 tgcgattttg gcgtttttta accataagct aatgtgatga tcaattttac cttatggtta
    32101 acagtctgtt tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt
    32161 ggctccgatg ctgggtttcg tggtgataat ttcaccatga aaaagttgtc agccccgctt
    32221 attcaatgag gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg
    32281 cgctcgcatg gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga
    32341 cgaacatcat gaggtacgta ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt
    32401 cgatcttcgc ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc
    32461 gaagctcacc aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt
    32521 gatagatgac ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc
    32581 cgccacccgc gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga
    32641 aattaccgtt cgtgatgacc tttccatgat cgccgtacaa gggccgaatg cgcaggcaaa
    32701 agctgccaca ctgtttaatg atgctcagcg tcaggcggtg gaagggatga aaccgttctt
    32761 tggcgtgcag gcgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta
    32821 tgaaattgcg ctgcccaatg aaaaagcggc cgatttctgg cgtgcgctgg tggaaggggg
    32881 cgttaagcca tgcggtctgg gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct
    32941 ttatggtcag gagatggacg aaaccatctc tccgttagcc gccaacatgg gctggactat
    33001 cgcctgggaa ccggcagatc gtgacttcat cggtcgtgaa gccctggaag tgcagcgtga
    33061 gcatggcaca gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga
    33121 actgccggta cgttttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg
    33181 gactttctcc ccgacgctgg gttacagcat tgcgctggca cgcgtgccga aaggtattgg
    33241 cgaaacggcg attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt
    33301 ttttgtgcgt aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc
    33361 aacgtaccag cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac
    33421 ggcacttaca ccgttggtat taccgaacat gctcaggagc tgttaggcga tatggtgttt
    33481 gttgacctgc cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa
    33541 tcagtaaaag cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac
    33601 gacgcactga gcgactcccc ggaactggtg aacagcgaac cgtatgcagg tggctggatc
    33661 tttaaaatca aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac
    33721 gaagcattgt tagaagacga gtaacggctt tattcctctt ctgcgggaga ggatcagggt
    33781 gaggaaattt acacctcacc ctcactctct tcgcaaggag agaggttcac aattcactgc
    33841 acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa tagcggcgct
    33901 tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt
    33961 ggcgcacaat cgttaaacgc gctgaccggc cagattgtac cgaaagatat tcagcttgcg
    34021 accccgccgc aggttggcgc accggcgacc gaatacgccg cgctggcgga actcaaggct
    34081 attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag
    34141 ttaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac cgcgtatact
    34201 ccgtatcaac ctgaagtctc gcagggccgc cttgaggcac tgctcaattt ccagcaggta
    34261 acgctggatt taaccgggct ggatatggcc tctgcttctc ttcttgacga agccaccgct
    34321 gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc
    34381 ttcgtggctt ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc
    34441 tttggttttg aagtgattgt cgatgatgcg caaaaagtgc tcgaccatca ggacatcttc
    34501 ggcgtgctgt tacagcaggt tggcactacc ggtgaaattc acgactacac tgcgcttatc
    34561 agcgaactga aatcacgcaa aattgtggtc agcgttgccg ctgatattat ggcgctggtg
    34621 ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttt
    34681 ggcgtaccga tgggttacgg tggcccacac gcggcattct ttgcggcgaa agacgaatac
    34741 aaacgctcaa tgccaggccg tattatcggt gtatcgaaag atgcagctgg caataccgcg
    34801 ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc gaactccaac
    34861 atttgtactt ctcaggtgct gctggcaaac atcgccagcc tgtatgccgt ttatcacggc
    34921 ccgattggcc tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat cctggcggca
    34981 ggcctgcaac aaaaaggtct gaagctgcgc catgcgcact atttcgacac tctgtgtgtg
    35041 gaagtggccg acaaggcggg cgtactggcg cgtgccgaag cggctgaaat caacctgcgt
    35101 agcgatatcc tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg cgaaaacgtg
    35161 atgcagcttt tcagcgtact actgggggat aaccacgggc tggacatcga cacgctggac
    35221 aaagacgtgg ctcacgacag ccgctctatc cagcctgcga tgctgcgcga cgacgaaatc
    35281 ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac
    35341 tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc
    35401 atgaaactga acgccgccgc cgagatgatc ccaatcacct ggccggaatt tgccgaactg
    35461 cacccgttct gcccgccgga gcaggccgaa ggttatcagc agatgatcgc gcagctggct
    35521 gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctctggcgca
    35581 cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg caacgaaggg
    35641 catcgcgata tctgcctgat cccggcttct gcgcacggca ctaaccccgc ttctgcacat
    35701 atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggaaatat cgatctgact
    35761 gatctgcgcg cgaaagccga acaggcaggt gataatctct cctgcatcat ggtgacctat
    35821 ccttccaccc acggtgtgta tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag
    35881 ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggttgg catcacctcg
    35941 ccgggcttta ttggcgcaga cgtttcacac cttaacctgc ataaaacttt ctgcattccg
    36001 cacggcggtg gtggtccggg tatgggaccg atcggcgtga aagcgcatct ggctccgttt
    36061 gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg cgcggtttct
    36121 gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct ggatgtacat ccgcatgatg
    36181 ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa ctatattgcc
    36241 agccgcctgc aggatgcctt cccggtgctg tataccggtc gtgacggtcg cgtggcgcac
    36301 gaatgtattc tcgatattcg tccgttgaaa gaagaaaccg gcatcagcga gctggatatt
    36361 gccaagcgcc tgatcgacta cggtttccac gcgccgacga tgtcgttccc ggtggcaggt
    36421 acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac
    36481 gcgatgctgg ctatccgcgc agaaattgac caggtgaaag ccggtgtctg gccgctggaa
    36541 gataacccgc tggtgaacgc gccgcacatt cagagtgaac tggtcgccga gtgggcgcat
    36601 ccgtacagcc gtgaagttgc ggtattcccg gcaggcgtgg cagacaaata ctggccgaca
    36661 gtgaaacgtt tggatgatgt ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt
    36721 agcgaatacc agtaattcac tgattcgact atcttctaaa ggcgcttcgg cgccttttta
    36781 gattgttaat aaagtgcgct ttgtgtgttt cggctgaggc gtaatataaa atattgcgag
    36841 ctcaatatat taaaagagtg ccaggcctga tgagcgtagc gcatcaggca catcggctaa
    36901 ttacagattc tcgccgttgc tggcaatcac ttctttgtac cagttaaagc tcttcttacg
    36961 tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg
    37021 tttgctgtac tgcccggtag tgaacgacac gcagtcgatg cagccccacg gtgtgtagcc
    37081 catcagatcc acgccatcgt aagttactgc ttttttcatc tcttcaatat gggcgcgcag
    37141 gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt cgtaagcgcc
    37201 aaaaccgttt tcgacaataa acagcggctt ctggtaacgc tcatacagtt cgcaaagtgc
    37261 gtaacgcaga cccaccggat caatctgcca gccccagtca gatgctttaa catacgggtt
    37321 tggtacgctg ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg ccttcactgc
    37381 gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcgcgca gtacatcgag
    37441 atcgctatct tccattttga tgttaaatcc gcgacgctcc cactcgttca acacatacga
    37501 cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca tcgactcctg
    37561 agcgaacatc acatcgtccg ggttacatga gtagggatag agcggcacca tcgccagcat
    37621 acagccgact ttcatctcca ggttaatgcg acgcgcggct ttcactgcca gggcgctggc
    37681 gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttttcat gctcggtata
    37741 caccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct ggttgttaat
    37801 ttcgttgaag gtcatccagt atttgacttt gtgcttatag cgttcaaata ccacttccgc
    37861 gaaacgtaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt gctgcaccag
    37921 atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt atttcagcag
    37981 ttcatcgaac agagagtcgt agaacttcag cccttcttcg tttggctgag cttcatcgcc
    38041 ttttggaaaa atgcgcgtcc aggcaatgga tgtacgaaaa catttgaagc ccatttcggc
    38101 aaatagcttg atgtcttcct tatagtgacc ataaaaatca acggcttcat ggtttggata
    38161 gtatttgcct ggcaagactt ctttggttat ttcgcgcggc acgccgtgtg cgccaccggt
    38221 cagaacgtca caaatgctcg gcccttttcc gcctttgttc cagccgcctt cgacctgatg
    38281 agcggcaact gcgccgcccc ataagaaatc tttcggtaag gtgagttttt tcactatcat
    38341 aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt
    38401 tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg
    38461 agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cggacgtttt gaagatgacg
    38521 gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat acgctgacgg
    38581 aaaaacatgc agagcaggaa aatatgaccc tgactgaact gaaaaaggtc attgccgaca
    38641 tctatcccgg ccagacacag ttttatgtga ttgaatttaa atgcctttaa attgaactca
    38701 cgcctgttag ttgaaatttc gcatttaatt tcatgatttt taggagatta gtctcaaatc
    38761 agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta
    38821 tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct cattaagcag
    38881 ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg gttggtgttt
    38941 ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca
    39001 gcgataacca gctacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg
    39061 ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc
    39121 gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg gctggattct
    39181 ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg gattctgttc
    39241 aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacttatct ggcgatgggc
    39301 tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc
    39361 ttactggcgg taggcggcgt ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc
    39421 attccataca accatgccat ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt
    39481 ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag gcctgataag
    39541 acgcgccagc gtcgcatcag gcgataaatg cctgatgcga cacagagatt tactcttcga
    39601 gcgaatacgg cagtggctcg atacacaatg tattcgcatc gtcgcgcacg cggaagatgc
    39661 tgtccggttc catatcgtta ttcatcacta cctgcaccac gacctgacca tcttccagtt
    39721 ttaccgcagc cagcaccgta ccagtacgac gccagttctc gcccattttc agctctaagt
    39781 cttcaccagc ttccggcagt cggctggcac tacctgtcag caaccagagc gcgcgtttgt
    39841 tggcaccacg gaattttgct cgcgccacca tctcttgtcc ggtatagcag cctttcttaa
    39901 agctgatacc gcccagcgcc tggaggttgg tcgcctgtgg gataaactgc ccgctgttcg
    39961 cggcatcaat caccgggaaa cccgcttcaa tgtttaatgc cagccactgt tggctattgt
    40021 tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt ggcagtagct tcatcggtta
    40081 cgatcaggaa acgttctgcc gggtgttcaa accatagcaa agtggtcgcg ccttctttaa
    40141 ctacctgttt ttctttcgaa ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct
    40201 gaaaaccggc aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc actttagaga
    40261 acaccgcata ttttttcagt tcagccagct gcggttcacg cacgctgcgc cgttcaatcc
    40321 atgcaaagcc atcgccgtcg cggaacagac gtaaattgct ccacatttta cctttggcgt
    40381 cgcaatgggc ggcgagcagg tgctgatcat ctgtcatctg gctgacatct gctgtcacct
    40441 ggccctgcaa atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa
    40501 gcgtcatcag cgtcagtggc aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg
    40561 taaaagccat aatagagtcc tgagttgctt aacggcgtat taataactaa tggtaaaaga
    40621 gctatgaggc aatgcaagtg ctttcactac gcgatctgtg ccttctgcga ggcgacttcc
    40681 caaaaaatga agcccttaaa ttgttgtgaa ttaatttcgc gatcgcgatt gtgtttactg
    40741 atattcccgt aaaagaagtc gaaaaacacg ttacaatagc cgggtactgg attttcgtga
    40801 gacaggaaga agatggacat taacaacaaa gcccgcattc attgggcatg ccgccgtggt
    40861 atgcgcgaac tcgatatttc aatcatgccg tttttcgaac atgagtatga cagcttaagc
    40921 gatgacgaaa aacgcatctt tattcgtctg ctggaatgtg acgatcccga tctgtttaac
    40981 tggctgatga atcacggtaa accggccgat gcagaactgg aaatgatggt ccgactcatc
    41041 cagacacgga accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc
    41101 acagtggctt tccttgctga ttcatgggct ggttgccgct gttattttac tcatgccctg
    41161 gccgctcagt tacaccccgt tatggatggt gttactttcg ctggtggtgt ttgattgcgt
    41221 tcgcagccag cggcgtatta atgctcgcca gggggaaatt cgcttgttga tggacgggcg
    41281 tttgcgttgg caagggcagg agtggagcat cgtcaaagcg ccgtggatga ttaagagcgg
    41341 catgatgctg cgtttacgtt ctgatagcgg taaacggcaa catttatggc tggcagccga
    41401 cagcatggac gaagccgaat ggcgggattt acggcggatt ttgttgcaac aagagacgca
    41461 aagataaaga tgacggcaaa gcatatgcgc taagccgtca gtgatatcag gcgtaatgct
    41521 ctgccatttc gttgaggatt tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat
    41581 actggttagt ttcgtccagc gccagcccca cgaacagttg cccgtcagca atcaccggtt
    41641 tcgggctggt aaattcatac ccttccgttg gccagtagcc gacgaacttc acgcctttgg
    41701 tcgagagttt gtcatgcagc ataccgagcg catcgaggaa ccactcgccg tatcccagtt
    41761 gatcgccaag cccatacagc gcaacaattt taccttcgag gttcaggtcg tcgagctgat
    41821 cccagacggc ttcccagtct tcctggattt cgccaaaatc ccaggtcggg atacccagaa
    41881 tgagcacatc gtactgctcc attaatttcg gggagtcgtc cttgaggtta tgtaaggtca
    41941 ccagttctgg gccgataata tcgcggattt tttctgccgc catttcggtg taacaggtgc
    42001 tggaaccgta aaaaagaccc atattcataa cgtaagcttc tcgattctgc tgtggctttt
    42061 gcggctagtg tatcagaatc gctttagtca cggcacaata aggcataatg gatcaaatga
    42121 gtgaccataa ggggcgcaag tgaaacagga tctggcacgc atcgagcagt ttcttgatgc
    42181 tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc
    42241 aatgatggtg gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga
    42301 tttacaggca ttactggcag aacggctgga gggcgggtat aaagccacca gctcagcgcg
    42361 tttgctgagt gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga
    42421 tgatcccagt gcgcatctcg cttcaccgaa attgccccag cgtttgccaa aagatttaag
    42481 cgaagcgcag gtcgaacgtt tattacaggc accattaatt gatcagccac tggagctacg
    42541 cgataaagct atgcttgaag tgttgtatgc taccgggctg cgtgtctctg aactggtcgg
    42601 gctgacaatg agtgatatca gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa
    42661 caaagagcgc ctggtgccgt taggtgaaga ggcggtttac tggctggaaa cctatctgga
    42721 acatgggcgt ccgtggctac tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg
    42781 tgcgcagcag atgacgcgac agaccttatg gcaccgcatt aaacattatg ccgtgctggc
    42841 gggtatcgac agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg ccactcactt
    42901 attaaatcat ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca gcgatctctc
    42961 caccacgcaa atttatacgc atgtcgctac cgagcgtctg cggcaacttc atcaacagca
    43021 tcacccgcgg gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag gttttatgtt
    43081 gtttactttg ttagcggcgt tttcaggctt cgctcaggct gatgacgcgg caattcaaca
    43141 aacattagcc aaaatgggca tcaaaagcag cgatattcag cccgcgcccg tagctggcat
    43201 gaagactgtg ctgactaaca gcggtgtgtt gtacatcacc gatgatggta aacatatcat
    43261 tcaggggcca atgtatgacg ttagcggcac ggctccggtc aatgtcacca ataagatgct
    43321 gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcac cgcaggaaaa
    43381 acacgtcatc accgtgttta ctgatattac ctgtggttac tgccacaaac tgcatgaaca
    43441 aatggcagac tataacgcgc tggggatcac cgtgcgttat cttgctttcc cacgccaggg
    43501 gctggacagc gatgctgaga aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa
    43561 agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg acgtggatat
    43621 tgccgaccat tacgcacttg gcgtccagct tggcgttagc ggtactccgg cagttgtgct
    43681 gagcaatggc acacttgttc cgggttacca gccgccgaaa gagatgaaag aattcctcga
    43741 cgaacaccaa aaaatgacca gcggtaaata attcgcgtga aacaacagat acaacttcgt
    43801 cgccgtgaag tcgatgaaac ggcagacttg cccgcggaat tgcctccctt gctgcgccgt
    43861 ttatacgcca gccggggagt acgcagtgcg caagaactgg aacgcagtgt taaaggtatg
    43921 ctgccctggc agcaactgag cggcgtcgaa aaggccgttg agatccttta caacgctttt
    43981 cgcgaaggaa cgcggattat tgtggtcggt gatttcgacg ccgacggtgc caccagcacg
    44041 gctctaagcg tgctggcgat gcgctcgctt ggttgcagca atatcgacta cctggtacca
    44101 aaccgtttcg aagacggtta cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt
    44161 ggcgcgcagt taattgtcac ggtggataac ggtatttcct cccatgcggg agttgagcac
    44221 gctcgctcgt tgggcatccc ggttattgtt accgatcacc atttgccggg cgacacatta
    44281 cccgcagcgg aagcgatcat taaccctaac ttgcgcgact gtaatttccc gtcgaaatca
    44341 ctggcaggcg tgggtgtggc gttttatctg atgctggcgc tgcgcacctt tttgcgcgat
    44401 cagggctggt ttgatgagcg cggcatcgca attcctaacc tggcagaact gctggatctg
    44461 gtcgcgctgg ggacagtggc ggacgtcgtg ccgctggacg ctaataatcg cattctgacc
    44521 tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc cagggattaa agcgctgctg
    44581 gaagtggcaa accgtgatgc acaaaaactc gccgccagcg atttaggttt tgcgctgggg
    44641 ccacgtctca atgctgccgg gcgactggac gatatgtccg tcggtgtggc gctgttgctg
    44701 tgcgacaaca tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct aaatcagacg
    44761 cgaaaagaga tcgaacaagg aatgcagatt gaagccttga ccctgtgcga gaaactggag
    44821 cgaagtcgcg acacgctacc cggcgggctg gcaatgtatc accccgaatg gcatcagggc
    44881 gttgtcggta ttctggcttc gcgcatcaaa gagcgttttc accgtccggt tatcgccttt
    44941 gcgccagcag gtgatggtac gctgaaaggt tccggtcgct ccattcaggg gctgcatatg
    45001 cgtgatgcgc tggagcgatt agacacactc taccccggta tgatgctgaa gtttggcggt
    45061 catgcgatgg cggcgggttt gtcgctggaa gaggataaat tcgaactctt tcaacaacgg
    45121 tttggcgagt tggttaccga gtggctggac ccttcgctat tgcaaggcga agtggtgtca
    45181 gacggcccgt taagcccggc cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct
    45241 ggcccgtggg ggcagatgtt cccggagccg ctgtttgatg gtcatttccg tctgctgcaa
    45301 cagcggctgg tgggcgaacg tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg
    45361 ctgctggatg gtattgcttt taatgtcgat accgccctct ggccggataa cggcgtgcgc
    45421 gaagtgcaac tggcttacaa gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa
    45481 attatcatcg acaatatctg gccaatttag cgtcatcttc tctataaaaa agagcgtgga
    45541 ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc
    45601 atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt
    45661 cttagggggt atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga
    45721 gctggaacag ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg
    45781 ttcctccctc gaagccgttg tcgacaccct cgaccaaatg aaacaggggc tggaagatgt
    45841 ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt
    45901 tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc gtatgttctc
    45961 tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga
    46021 agcacaggac tgggcgagca tgcttgagcg tatgtatttg cgctgggcag aatcgcgtgg
    46081 tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac
    46141 gatcaaaatc tccggcgatt acgcttacgg ctggctgcgt acagaaactg gcgttcaccg
    46201 cctggtgcgt aagagcccgt ttgactccgg cggtcgtcgc cacacgtcgt tcagctccgc
    46261 gtttgtttat ccggaagttg atgatgatat tgatatcgaa atcaacccgg cggatctgcg
    46321 cattgacgtt tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc
    46381 ggtgcgtatt acccacatcc cgaccgggat cgtgacccag tgccagaacg accgttccca
    46441 gcacaagaac aaagaccagg ccatgaagca gatgaaagcg aagctttatg aactggagat
    46501 gcagaagaaa aatgccgaga aacaggcgat ggaagataac aaatccgaca tcggctgggg
    46561 cagccagatt cgttcttatg tccttgatga ctcccgcatt aaagatctgc gcaccggggt
    46621 tgaaacccgc aatacgcagg ccgtgctgga cggcagcctg gatcaattta tcgaagcaag
    46681 tttgaaagca gggttatgag gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc
    46741 ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga acctgcgtga
    46801 gcagggaatt gccttcccga acgatttccg tcgcgatcat acctctgacc aattgcacgc
    46861 agaattcgac ggtaaagaga acgaagaact tgaagcgctg aacatcgaag tcgccgttgc
    46921 tggccgcatg atgacccgcc gtattatggg taaagcctct tttgttaccc tgcaggacgt
    46981 tggcggtcgt attcagctgt acgttgcccg tgacgatctt ccggaaggcg tttacaacga
    47041 gcagttcaaa aaatgggacc tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac
    47101 caaaaccggc gaactgtcta tccactgtac cgagttgcgt ctgctgacca aagcactgcg
    47161 tccgctgccg gataaattcc acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta
    47221 tctcgatctc atctccaacg atgaatcccg caacaccttt aaagtgcgct cgcagatcct
    47281 ctctggtatt cgccagttca tggtgaaccg cggctttatg gaagttgaaa cgccgatgat
    47341 gcaggtgatc cctggcggtg ccgctgcgcg tccgtttatc acccaccata acgcgctgga
    47401 tctcgacatg tacctgcgta tcgcgccgga actgtacctc aagcgtctgg tggttggtgg
    47461 cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca
    47521 taacccagag ttcaccatga tggaactcta tatggcttac gcagattaca aagatctgat
    47581 cgagctgacc gaatcgctgt tccgtactct ggcacaggat attctcggta agacggaagt
    47641 gacctacggc gacgtgacgc tggatttcgg taaaccgttc gaaaaactga ccatgcgtga
    47701 agcgattaag aaatatcgcc cggaaaccga catggcggat ctggacaact tcgactctgc
    47761 aaaagcgatt gttgaatcta tcggtatcca cgttgagaag agctggggtc tgggccgtat
    47821 cgttaccgag atcttcgaag aagtggcaga agcacatctg attcagccga ccttcattac
    47881 tgaatatccg gcagaagttt ctccgctggc gcgtcgtaac gacgttaacc cggaaatcac
    47941 agaccgcttt gagttcttca ttggtggtcg tgaaatcggt aacggcttta gcgagctgaa
    48001 tgatgcggaa gatcaggcgc aacgcttcct ggatcaggtt gccgcgaaag atgcaggtga
    48061 cgacgaagcg atgttctacg acgaagatta cgtcaccgca ctggaacatg gcttaccgcc
    48121 gacagcaggt ctggggattg gtatcgaccg tatggtaatg ctgttcacca acagccatac
    48181 catccgcgac gttattctgt tcccggcgat gcgtccggtg aaataagcat tacgttatgc
    48241 gcataacccc ggcagatgtc ggggtttttt aattgtgctg cgcgaaagca gacaacaatt
    48301 ttcttgcttc actattggct gcctgcatca ccatccacgg actgaacgcc cacggcgtgg
    48361 catcaatacc gtgtaataca tctgctaaat cacaccattg ataatccatc acttcatcat
    48421 cgttgatctg taacgcactg gtggtgcgtg cggcaaatac cggacacact tcattttcca
    48481 caatgccatt cggatcggtg gcgcgatagc gaaagtcagg atagatagat tcaggaggcg
    48541 taatttccac gccaagctca taacggcaac ggcggatcac cgcgtcttcg ttgctttctc
    48601 ccagttgtgg gtgcccacaa accgagttag tccacacgcc aggccatgct tttttgctca
    48661 gtgcgcggcg ggtaactaat aattgtcctt tggcattaaa cagccaactg gagaacgcga
    48721 gatgtaagcg ggtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc
    48781 cctgtgcatt caataaaatg acgtgttccg tttgcataat ttctcacatg taattctgat
    48841 cactcatcat tatagataat tgaagagtga aatgattaac caagtgagcg tttatcgaca
    48901 accgcccgtt ttgagcggat gccgacaggt aaaaaccatt taaaaaagta gcatcatata
    48961 tttgaagcga ctttcaatgg ccattgcaac acagtcagaa atgacaggaa tagagagagt
    49021 aaaaagcaac agcagactga atatagcgcg cattaaaaaa ttcatattat tctccatgct
    49081 catttttctt cagcttgcgc atcagaagcc acatacgtac tgtcctgact tttaaatctt
    49141 tatcagagac ggattccttc accaggtcag catgatgctg atagccatta aagaagacgt
    49201 tcagcacaac ggcgctaagg gttgcgagca taatgccgct atgtagcagc ggttgcagta
    49261 cggccggtaa cttagaaaag aaatcgtgag agagcgttgg agtcatgcca acgccgagac
    49321 tgatcgccac aatatagagg ttgtaacggt tggtggtgta gttacagcgc gacagaattc
    49381 gaatccctgt cgccagtacc atgccgaaca tcaccagacc agcgccgccc agcacaaatt
    49441 gcggaatgga ggctaccagc accgccattt ttggcaccat gccaaacagg attaatataa
    49501 ttcccgaaga aatacacacc cagcggctat gaacgcgcgt cacgctaacc aggccaacgt
    49561 tttgagaaaa tgacgtgtgg gggaagctgt taaacgtgcc accaatcatt gtccctacgc
    49621 catcgacacg cagcccgcga ataatatcgt gcgaagagag tttacgaccg actatttcac
    49681 ccagtgccag gaacatcccc atcgactcga taaacacgat gattaacacg gcagtcatgg
    49741 tcagaatgga aacgggatcg aaaatcggca taccaaacga catcggggtg acaatcgcaa
    49801 accacgaggc atcatgtagc ccggataaat tgacttcatt catcatccac gaaagtaaaa
    49861 agccaaatac aatccccagt aatacggcga cgttggacat aaatcctttc gcatagcgag
    49921 taatgagcaa gataaaaatt aagacggcaa aggagatacc taaataaacg ggattaccat
    49981 attgcggatt ccctttacct ccggcggccc agtcaatacc cacctgaatg atgctaagcc
    50041 cgatagaagt aataaccaca ccggtaacca gtggcgggaa taaaggcatc aagcgaccga
    50101 taagttgcgc taataatgtg gtgataaaac ccgcggcgat agtggcacca aatataccca
    50161 gcaggccgat atccgggttc atcccaatgg ctatcattgg tgttacagca gcaaaggtca
    50221 ccgacataat caccggcagg cggatcccca taaagcggcc gataccgata cattgcaata
    50281 atgtgacgat cccgcagcaa aagagatccg agctaatgag catcgcaata gcttcttttg
    50341 agaggcccag tcggtcacca atcataagag gaacagcgac tgcacctgcg tacatgacaa
    50401 ggacgtgttg cagaccgagt atgatcagct ttcctggtga taatatgcgg tcaacctcat
    50461 cagttgggcg gtcttgccca gaagatgagg gaagttggga atctatggcg ctcatcagtg
    50521 tttctccttt cgcccgccag agtaaaaagc aaccgtttac gttaatgaaa ctgtctaaac
    50581 acatgctcgt ggagtgccag tttttgcgag gaaggggagt tagagacagg aattgcggag
    50641 gcaatcacat aatcgtgctt aaactacgaa aatgacaaga gaacctgaca atggaattat
    50701 ctgtaatttt ctcatattga tatttcatta atcattctgg catgtcgcac tctcgcattt
    50761 aatcgttttt atctggatag cgctatttgg ggcggcaatg ttgaaataat gaaagcggca
    50821 tagattgagg ggcaataatt tgaactcagt cgcaaaatta taattaaaac ggtcatgcta
    50881 cctaatatat gcccgtttat tgcatcgcga aatttgagaa gacataactc attaaatgaa
    50941 agggatgcaa atgaataagt ttatcgctgc tgaagccgcg gaatgtatag gctgtcatgc
    51001 ttgtgaaatt gcctgtgcgg tggcacacaa tcaagaaaac tggccgctga gtcacagtga
    51061 ctttcgacca cgtatccacg ttgtagggaa aggcctggct gcgaatccgg tggcctgcca
    51121 tcactgcaac aatgcccctt gcgttacggc ttgtccggtt aatgctctga ctttccagtc
    51181 cgatagcgta caactggacg agcaaaaatg tattggttgt aaaagatgcg caatcgcttg
    51241 cccctttggc gtcgttgaga tggtcgatac gattgcgcag aaatgcgacc tttgtaacca
    51301 gcgcagttcc ggcacgcaag cctgtattga agtttgccca acacaggcgt tacgactgat
    51361 ggacgataaa gggttacagc agataaaggt ggcccgccag cgcaaaacgg cagcaggaaa
    51421 agcgtcatca gacgctcagc catctcgcag tgcagcgttg ctccccgtta actcgcgtaa
    51481 aggcgcagat aaaatttcag caagtgaacg gaaaaaccac tttggcgaaa tctattgcgg
    51541 gctggatcca caacaagcga cttatgagag tgaccgctgt gtttattgtg ccgaaaaagc
    51601 taactgcaac tggcattgcc cgctgcataa cgctattccg gattacatcc gtctggtaca
    51661 ggagggaaag attattgaag cggcagaact ttgccaccag accagttcct taccagaaat
    51721 ctgcggcagg gtatgtccac aggaccgtct ttgtgaaggc gcatgtactt tgaaagatca
    51781 ctctggcgca gtctctatcg gtaatctgga acgctacatc accgataccg cgctggcgat
    51841 gggctggcgt cccgatgtca gcaaagttgt tccccgtatc gagaaagtgg cggtgattgg
    51901 cgctgggcct gcagggttag ggtgtgctga tattctggcg cgcgcaggag ttcaggtcga
    51961 tgtctttgat cgccatccag aaattggcgg tatgctgact tttggcattc ctcctttcaa
    52021 actcgataaa acggtattaa gccagcggcg agagatattc accgcaatgg gaatcgactt
    52081 tcatcttaac tgtgaaattg gccgcgatat tacctttagc gatttaactt ctgaatatga
    52141 tgcagttttc atcggcgtgg ggacttacgg gatgatgcga gcagatctgc cgcatgaaga
    52201 tgcgcccggt gtcattcagg ctctaccgtt cctgactgcc catacccgcc agctcatggg
    52261 attgccggag tctgaagagt atccgctgac ggacgtggaa ggtaagcgag tcgtggtatt
    52321 gggcggtggc gatacgacaa tggattgttt gcggacttcc attcgcctca atgctgccag
    52381 cgtgacctgc gcgtatcgtc gtgatgaagt cagtatgccg ggctcgcgca aagaggtggt
    52441 caatgcgcgc gaggaaggtg tcgagtttca attcaatgtt cagccgcaat atatcgcttg
    52501 tgacgaagat gggcgtttaa ctgcggtggg cctgattcgt accgccatgg gtgagccggg
    52561 gccggatggt cgccgtcgtc ctcgtccggt tgcgggttca gagtttgaat tgcccgccga
    52621 tgttctcatt atggcctttg gtttccaggc gcatgccatg ccgtggttgc agggcagcgg
    52681 aattaaactc gataaatggg gcctgattca aaccggtgac gtcgggtatt tacctaccca
    52741 gacgcatctg aaaaaagtct ttgctggtgg tgatgcagtt catggcgcgg atctggttgt
    52801 cactgcaatg gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt ttgatacgaa
    52861 ggcatcgtga tgaaatcgtt aattatcgtt aatccggctg actgtattgg ctgccgtact
    52921 tgtgaagtgg cctgtgtggt cgctcaccct tcataacagg agttgaatgc cgatatcttt
    52981 ttaccccggc tgaaggtaca gcgactggac tgcatcagtg cgccggtgat gtgccatcag
    53041 tgtgaaaacg ccccttgtgt tggcgcttgc cccgtggggg cgctgacgat gggcgagcag
    53101 gtggtgcaga ccaattctgc ccgttgtatt ggctgtcaga gttgcgtcag tgcgtgcccg
    53161 tttggaatga tcaccattca gtcgttgccg ggggataccc ggcaacaaat cgtgaaatgc
    53221 gatctctgtg aacagcggga agaggggcct gcctgcgttg aatcttgccc aacacaggcg
    53281 ctgcagttgc ttactgaaag agaactcagg cgagtccgcc agcagcgtat tgttgccagc
    53341 ggtgagaatc ctctgtgaaa gaaatctccg cgctgaaagg cgcggagaat ctattagatt
    53401 gcccaaccac ccgcgtaaaa tgcgaccagt gcggcagtaa tgatcaccgt accaatattc
    53461 agcttttgcc attcgcgtgc aaagacgcgg cctacgacca gtgtcacaaa gcccagcata
    53521 ataccggtaa cgatattaca agtcagaacg atgaacacgg cacacaccag gccagccata
    53581 gcgtcaataa aatcattgaa atccagcttc gagacgttac ttaacatcag caaacctacg
    53641 tacatcagag cgggtgcagt ggcgtaacca gggatcaaaa atgacagcgg tgataagaac
    53701 agaatcagca ggaataacgc ccccactacg gttgcggtta accctgtttt acccccggcg
    53761 gcggttcctg ccgctgattc gatataaacc gctgcgggcg ctgcgcccac caggccggag
    53821 aatattgaac ttactgagtc actggtcagg gctttgccgc cgttgatgat ctggttgtct
    53881 ttatccaaca aattcgcctg accggcgacg gcacggatgg tgccagtagc gtcgaacact
    53941 gcggtcatca ccaatgccag tacactcgga agtacagttg gctggagtgc gcccataata
    54001 tcgaggctga aaatcagaga cttaccatct tcgccagtca ggcttggcat cgccaccaga
    54061 ccgtggtatt tcaccgctgg gtcaaagatt aagccgatga tcgaaattgc aataatcacc
    54121 aacaagatac cgccgggtac gcgacacttc tccagaccga agatgaccgc cagccccagc
    54181 aagctcatca tcaccgggaa ggaggtaaac gcaccgagcg ccactggcaa gccttcaatc
    54241 gggtttttga taaccatacc cacaccgtta gcagcaatca gcagcagaaa cagcccgata
    54301 ccgatacctg taccgtgagc gatacccatc ggcaaattac gtaagatcca ggtacgcaca
    54361 ccggttacgg aaatggcggt gaagatgacg cccatcagaa ataccgcgcc cagtgcgaca
    54421 ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg taaacgccgt caaggaaatc
    54481 gcgcaaccaa tcgccattgg caaattagcc cataacccca tcagcaacga gccgaagccc
    54541 gcgaccagac aggtggcaac aaacacagct gcgggaggaa aacctgcttt gcccagcatt
    54601 cccggaacga cgataacgga ataaaccatg gccagaaagg tcgttaagcc agccagtact
    54661 tcctgacgaa cggtactgcc acgagcggta attttaaaat aattatccag cgcgccctgt
    54721 ggctttggtg cgtccggtgt ttgtaggatg tctccagaca taatgatgtc ctcacgagga
    54781 tgtaaaagtt ttattattag ttgcgttcgt acaccagacg accatcaacg taggtgcggt
    54841 agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact aaagaaacag
    54901 agttgtcata gcgcagctgc tgtagcggag tggcggtggg ttccatcacc acgaaatcag
    54961 cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag gcccagagat ttcgctccgc
    55021 cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg atagccttgt aattgcaaca
    55081 ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt ggttccggca ccgatatccg
    55141 ttcccatgcc cactttaact ttcttctgcc atgctttttt caagttgaat aacccgctgc
    55201 cgaggtaaag gttggaggtc ggacagaaag caatgctgga tttggtttcg ctgagacgat
    55261 cccactcttt ttctttgaga tggacgcagt gagcaaagac acagttttta ccggtcaggc
    55321 cgtactgatg gtaaacatcc agataaccat catggtcagg ataaagcgat ttcacccagg
    55381 caatttcatc tttgttttca cagagatggg tatgtaccca cgtatccgga tattcctctt
    55441 tcaggcgttg cgccatcgcc atctgttcag gagaagaagt aggggcgaag cgtggcgtaa
    55501 tcgcatatag cagacgacca tttttgtgcc agcgttcaat cagttctttg ctttggtgat
    55561 agctgctttc ggcagtgtct agcagataat ccggtgcgtt gcggtccatc atcaccttac
    55621 cggcaatcat acgcatattg atatgactgg cggcttcaaa cagcgcatca acggattgcg
    55681 gatgaacagt gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc tgcttgatga
    55741 agaacgccga catttcgcgg gcgtactcta aatcctcata acgacgttca gtagggaagg
    55801 tgtgtttatt caaccactcc agcaattgtt ccccataggc ccccaccatt tcactttgcg
    55861 gataatggat atgtgtatcg acaaagcccg gtactatcag tttgccgcga tagtcgcgca
    55921 cgcgaatagt gtcaggaatt tgatgctttc cgtcttccca ttcgccaaac cattccactt
    55981 ttccctgttt aatgagtaat aaaccatcct caataaaccg cagcgcagag gcaatctctt
    56041 ccgggttatc gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct tttaacgtgt
    56101 gttctcctga catcattgac tcctttactg ttaatccatc tcttccgctg aggtaatacc
    56161 aggcagaacg ttttcctgtc ggtagccacc agggaggata atatttaata aaatcgcagt
    56221 taacccgcca gcacaaatag ggttttcaac taatacataa atagaggctg gcaatatttt
    56281 aaaaatttcg ggatcgtagg agacgccaag tcctaaacct aaagaagtgg cgacaataag
    56341 tgtttctcgg cgctttaaac cgttggtgat gatgatgcga atcccagcga tggcaatcat
    56401 ggaaaacatc aacgtcattg cgcctcccag aactgccgag ggaatggtcg tgaagaagcc
    56461 gccaatcatc ggaaacaaac cgaggataac cagcattacc gcgatggttc gcccgacata
    56521 acgtgaagcg acgccagtca tctgaataac cccattattt tgcgcaaacg tggttaaggg
    56581 taatgacccg acagcggagg cgataacaga aaccagaccg tctgccagca cgccgccttt
    56641 cagtcgggac tgatactctt ctccctgaat gggtcggcgg gaaaccattg ccgtggcggt
    56701 gatatcgccg acagcttcca gcacgctaag cagataaatc gtgccaacca ccaggaactg
    56761 atggaagcta aaactaaagc cgtatttgaa cggatgtggg atggtgatta acggcaaatt
    56821 gcgcatactg ctgaaatcca ccatgcccag gcataacgac gcgatataac cgacgcatag
    56881 cccaatggcg atccctccca tgcgtagcaa cggactgcga cagcagttaa agccgatcac
    56941 cacaatcaaa accaataaac caacgccgag atgttcgtaa ttgccgaagg taccgctgct
    57001 tttggcagca aatcctccac caaaatcgat aatgccgact ttaatcaggc ttaagccgat
    57061 catcagtacc acaataccgc tgacagtagg cgtaataacc cgacgtaaat agggcaagat
    57121 aaatgaagat ccgacaacca gaaatgcgcc aacgaaggag acgccgagga gcgacgacat
    57181 gattaactct tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa tcatgaccgt
    57241 aacaaatgaa aaattgactg actgaattga gagtaggcca gaaccgacga tgccatagcg
    57301 gtttacttgt aaccaggtac caataccaga ggcgatcatc gccattgaaa caagataggc
    57361 agttgtttca gcggaaagct gtaaggccgc ccccacgatt aacgcggggg ttaccatcgg
    57421 aacgaaaatt gccaacagat gggtaatggc accaacgaga gcctgatgaa agggagggcg
    57481 atcctccagt tcaaatataa ggtcagaacc tgcatggttt atatcagaca tcccttcact
    57541 ccttttaaag taaatttgat catctgaatt agtaaaataa attcccacga atttggggcg
    57601 caggacgcct tgagaacggc ttatctcatt ttcgcagtca ctatttagtc atcgtccaaa
    57661 cagagaaaca ggccgccgaa gcggccattt attatttata tcttttccag cgcggtgagt
    57721 attttctccg gtgtgaaatg ccattcgcgt aaccagatgc cgcatgcatc gtgaattgcg
    57781 gtagcaatcg ccggagctgc gccatttaca ccgatttccg agatcgattt cgccccgaac
    57841 gggccgactt tatcgtcgct cggcaccagc actgcgcgga aatcacgcgg aatgtcacca
    57901 attttcggtg cgccgtagct gcgtaaatca cgcgttaacg ggtgaccttc ggcgtcataa
    57961 atgatctctt cgctcatact gtggccgata gctcgcaggg tagcaccgta gatttgtccc
    58021 aacgctaact ctggattgac cggtgtaccg cagtccagca aggcgtagaa tttatccagg
    58081 cggatttcac ccgtacgcgt gttgacggca acttcagcga agtttgcgcc atacgggaag
    58141 gcgaaatcag gcgtgatata actgccagtt cccaccagtg aaccaaagcc ggtgccggtt
    58201 tcgcctttat gggcaatatc cccgaaacta acttcgcctt tcttgccgcg cacgacgccc
    58261 ggcgttgcta gttgaacatc tgccactggc tcacccaaca tttgcgcgcc gtggaacaga
    58321 attttctccc gcagattttc cgctgccaaa cgcgccgcgt tacccgagaa gcaagtaccg
    58381 gacgaggcat atgcgccttt atcaaacaac gcatgatcgg tatcaccgga gataacatgc
    58441 acgtcctgcg gtgggcagtg cagcacttct gctgccagtt tcgtcaccac ggtatccaga
    58501 ccagtaccaa tatccgcacc gccagaatga acgataaagg taccgtctga ttccagtttg
    58561 atcatgcagt tagcctgatc gatatccggg atccctgatt tctgcatgat aatggcgaca
    58621 ccacgaccga tgtgccagtc accattttgt ggttttggtg aagaccattg gatcatctcg
    58681 cgcccctgac gcaggatctc ttccagtgcg cagctggcgg cggaaggaac ggaggtcggc
    58741 gctttacctt caccgattgc accgagaatt ttcagctctt gcccttcgtg tacccggtta
    58801 cgttcgataa tttccagttg gtcgatctgt aactgttcag ccagttccgc taatgccatg
    58861 gtgatagcga agttaccttt cggtgcgcca taaccctgat aagcaccatt tgggcaaatg
    58921 ttgctgtagt aggtggtgac ctggaaatcg acgttatcgc acggatataa cggcagcgac
    58981 agcgccggtc cgttacacgg tacggtgagt gagtggttgc cgtaagggcc agtgttggcg
    59041 cggaaatcca tcttcactgc cgtcaggcga ccatcttttt tcgctcccag tttgacggtg
    59101 actttcgcga cgtgacgaga ggtgttagca ataaactctt cttcacgggt gtagcggaac
    59161 agtaccggac gcccggtcac gcaggttgcc caggcgcaca cttcttccag caggatgtcc
    59221 tgtttggaac caaaaccgcc gccaactcgc tctttaatga catgaacttt atgctgtttc
    59281 atgtccacga ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt ggaggcgtgg
    59341 ataaccagac gatcgccgtc catccgggta aagcagatat gtgtttcagt cgggcactgc
    59401 tgcgcctgcg ttgagttata ggttcgctca atgatcacat cggcatcggc aaagcctttg
    59461 tccatatcgc caatatgacc atgaatacta gcggcgatat ttttgcgagg gcgagaaccg
    59521 atcgggaagt tgatgatcat atgctcgccg cgctgggctg catggctgtt atcgtcttcc
    59581 agagtatctg gcgcaccagc aacatacacc accggttcat cgtgcacgac aggcgcatct
    59641 tccgccattg cttcgtcgat cgacattacc ggcttaagca cttcatattc aacctcgatg
    59701 agtttcaatg cttcgagcgc aatttcttca ctttcagcga ccaccgcagc aacgcgatcg
    59761 ccgacgtgac gcattttttt gccgaacata cggcggtcaa gcggtgacgg ttccggtgcg
    59821 ctctgacccc ccggggtata gtagatatca gggcaattca ggtgagtaat aacgtgaacg
    59881 acgcccggta aggcttcagc tttgctgaca tccagatgag taatcagtgc gtgagcgtgt
    59941 gggctacgta acattttaat gacgcaggcg tcagccgtta cgcggtcttc aacatagcag
    60001 ggtttcgcct gcaccatttt cgcggcatca gttttaggat aatgcttgcc aatgacttca
    60061 aggtcgtcac ggaaagtcgg agcgatatca atggtggcct gcggattatt tttacgtgca
    60121 accgccagtt caatgacctg atagtattgc tgccagccag catcgcggct gaacaaacca
    60181 gaaagcgcgt cgtcgatctc ttcgcgggta ggtgcggcga tgcgatcgag aagatcggtg
    60241 ataatcagag ctgcagctgg atcgttataa ccagactgca ccacgccaac atcaaccatt
    60301 gcctgttgaa ccagacttaa ctcgttccat ttgcccagag attctgcggt acgaatatct
    60361 gccttctcta actgtgcggc aataagcaag gacgcgttaa cgatattacc gttaaagatg
    60421 attgcgtcag aaccggcaaa cccgaaacca tcatcactgt tgcgtacaga gtgcattccc
    60481 atgttaaaca acagcttttg cacgttttcg cctggattaa cggttagctc ctgaggcgcg
    60541 ccatttaaag taaagtggat gatcatacgg cttcctcccc agcctgttgg cagtcggcat
    60601 acaggtcggc taccagaact cccgtgatat agcgtttata ggccacgctg ccccgcaaat
    60661 cttcctgcgg gaaaatggcg ttggcgacag cctgttcaag tgcattgcct tccagatttt
    60721 gtttttcgac atcatgcaga cgcagtgctt tactggcgac gccatccagc gcaattcgca
    60781 taccgtcgtg gtctgtcatt gcaacggctg ccgtcacgac ggttaaacct gcctgagaac
    60841 ggctaatttt gcgggtcgca caggtgcgat acggatcttt aatgataatt tcggttagca
    60901 ggcgatcgca tgggcaggcc aggtagtcct cgattgacag cgtttcgccg ttgccaaaaa
    60961 ccagttcagc atccagtgcc agcaggacgg gaagcagcac tgactcttcc tgccgggcgg
    61021 cgatttcacc accaatggtc gactgattac gaacatggcg tgagtaaaca aaaccgaggg
    61081 cttcacgcag tgctgcagga ataaatcgcg catcacgcag tggctgcaag cgagacattg
    61141 cgccaatccg cagtgcaccg ttatcccagt caacccagtc cagttccaga tcctgtaagg
    61201 aaatggcaat ctttttatcg gtacgggttg gtgtagcatt gagtttgctg cccccggcga
    61261 accagacggc ttcatcctgg tagcggcgct tcagttccag cgcctgttcg acagagtcgg
    61321 gcctgaaaaa ttgttcaatc atcttctttc cttatttaac cctcttcctg cgggaagagg
    61381 gcatctgtca tttatgccag cgcatccatc cgacgccaca tactggcagc ggcttttctg
    61441 gcttgtgcat aaatggaatc gcaatcgaag ttaaactgac ggtcttcata gaccatcaca
    61501 ccattgacca tcacgctgtg aacgctgcct gagcccatac cgaaagcgat atgcccggcg
    61561 atattgtctg ccagcagcgg cgtcggcgag ttgtaatcgc aaatggttaa atcagctttg
    61621 taaccggctt ccagaagccc aaatttcgcg ccaaagttgc ggctcatcag ttcgttaccg
    61681 ttagtcaggg ctttggcaaa actgtcaggc cacagcggac cacccgcatc gcgatgttta
    61741 aagaaggcaa atttcatctc ttcaaacatg tccgaaccaa tgccgtccgt tcccaacgcc
    61801 agattgcgga tgtcgctaag gtgatggttg tagccgacat ggttgttcat gtttgaacgg
    61861 gcgttatgca ccaggaacgc atcgcgctga ttgagtaggg cgatgtcatc tttcgacaag
    61921 tacagcccat gagcgaccag cgttttgcta tcgatgagat cgaattgcgc cagtcgtgcc
    61981 agcaggtctt tgccgtacca gtggtgactg taggaaacgt cgtaaaggtc ttccgcagcg
    62041 tgaatatgca aaccacggcc tgtggcgttc acggcttcac gtagcatctc cagaccggca
    62101 tctggcacgg taaacggcgc gtgagcaccg atatgtgctt ccaccagata cggctcggtt
    62161 gctgctttct tcgcctcatc aatctgacgg gcgaagcgga tgttttcttc tacaccttcc
    62221 tgcaactctt tgatgccgtt gttacggtca gtagtttcaa aacaggtcat cgcgcgcagg
    62281 ccaactttta aaaatgcgtc gcgcaatgtg gagagcgacc cgccgatata cgccggagag
    62341 gcatggtgat cgataaccga tgtacatccg ctcttaatcg cttccaggga acaaatcagc
    62401 ccgctgtaat agagcgactc ttcatcaagg gcgcgatcga gccgccacca gagatttttc
    62461 agcgttgaga tgaaatctgg gctgggggcg atgtttgcca taattccgcg ggaaagcccc
    62521 gagtaaaaat ggttgtgcga gcagacaatt cccggcatca caatccgacc atgcatctct
    62581 ttgtagctgg catcggggta gcgttgcgtc agggcatcgc cgatagcgac aatcacatca
    62641 ttttcgatag cgatatcaac gccttcctgc actttcgccg ggtgtaactg cactgcggtg
    62701 acattcttca gaatcaacat gattacacct ccacgcggcc cagcagataa tgatgatgct
    62761 ggtggacatg gctgatgatg cggcacatat cgttcagctc cggtggtacg ttgttgaact
    62821 gaccttcgct gtcgatgttt aacacccagc tttggttatt cagtcgtact cgtacccggc
    62881 aatcttccac aaggaagcct gggttgctgc tgttatcaaa gtcttgcgcc aggctgaaaa
    62941 cggtgatttt gtctttgtat ggtttaccgt tccacggaca gaactgagcg cagttgccgc
    63001 attcgttaca gtaagcgtcg aggtgcagcg tctggaatct attctggaag cctgggaccg
    63061 caatggagac gttggcgcgg ttcgggcaga catccacaca cttgctgcaa acgtagttac
    63121 attcaaggca gcgagcagct tcctgcgcga caaacgcatc acgatcgtca ctgttcacca
    63181 gcgtgataga gatatcgcct ttacgttgat agatttccgc cggattgacg ttgttccagt
    63241 atttatcgtt ctggtgggaa cggatatttt cccgactaag gatggcatcg gtcgcccgac
    63301 gcgcggttcc gacagcagcg acaatggagg atggtccgcg ctgtacgtcg ccgatcataa
    63361 agacgtcagt cagacgagtt tcgccattat ggtcgacgtc tggccagccg tttttgtcca
    63421 gcggcacgcc catcgcattc agggcttcag tatcctgctg ttcaccaatg gcggtgatca
    63481 ggctgtctac atgcagtgtt actgtttcgt tggtttcaac cggacgacga cgacctttct
    63541 catcgggttc gccaagcgac ataacgcgca aggttaaggt gccatcagca tcgaaacgtt
    63601 ccggattatt caggaaacgg aactccacgc cgtcgtgcaa cgcttcttca tactcttcgc
    63661 gccatgcggg catctcttgc agtgaacgac ggtaaacgac cgttgctttt tctacgcctg
    63721 gaacgcgtaa cgccgcacga gcgcagtcca ttgcggtgtt acccgccccg acaacgacca
    63781 catgtttgcc cagcttgagc gctgtaccct tgttgtattc acgcaggaag gggagtgatt
    63841 tcaagacatt ttggttgtcg cccgccagtt tcacaccgct atttttatct gtgccggtgg
    63901 caatcagaac atagtggaag tcctgatttt ttaactgctc aacggttaaa tcgggtgagc
    63961 agccatactc aaatttcacg ccgtgagcgg caacaaaatc gatatcgtgc tgaattaact
    64021 cagccggaat tcggaactga ggaatgatat ttttcaccac gccgcccgca ttggcttcgc
    64081 gttcaaacag cgtaaccgga tggcccgctc tggcaaggaa gtaacctgct gccagacccg
    64141 ccggacctgc accaatcacg gcaaccggat ggcgtgaacc agaaccggct ggtttgtgcc
    64201 agcgttgctt atattcatcc caaccttttt ccagcgcgac ttttttcagt tcgcggatat
    64261 tcagcgcact gtcgtaatcc aggcgggtac agttgtattg gcactggtga tcgcaaatat
    64321 gaccggtaat ggcgggcaga gcgttgcgtt ggtagatgag ttccagcgcg tcggcatagc
    64381 ggtgttcgcc aagcagacgg atgtattccg gaatatcttg cttgatagcg caggcagtaa
    64441 cacagggggc aacgtagcag tcggtcagcg gcaggtcttc tgccacttca atacgctctt
    64501 ctggcttcca gtgtttctgg gtgtattcca tggttaacgc atctgctgcc agtctgttca
    64561 gtcgttcgac gtcaacatgg tcaagtcccc aggcgtcgga gccttccagc tcgcgcatgc
    64621 aggcacttaa gcgcaaatag ccgccaggtt tcagcaggtc ggttgccata gtaatagggc
    64681 gaatacctgt atcaaaaata tcgcggatag tcagctgact ggcaccaccg gaataagaaa
    64741 tgggcagttt gccgtcaaag gcgcgagaga gaactgctgc aacattgatg gagagcggga
    64801 acagcgcacg gcctgacata tacatctctt caccaggcag tgcgccttta ttgttgatgg
    64861 tgccgagagt gttagtcagt tttacgccaa agccgagtga tttttctttt gccagtgcca
    64921 tcaggcgttc cagcatttcc agtgcttgcg tcagcttgag gtcgtgatca aatgactctt
    64981 cttttaagcc tatgtaaccg aaaccgcaga catcgaggat ctcacgaaca cgcgcgtacc
    65041 ccagtaaggt cgggttaagt ttcacaaagg tgttgagccc tttttcttcc agcatgtagc
    65101 ggcaaatggc ttcgatttca tgcggaggac agccgtgcat ggtggagagg gtgacgccat
    65161 gcaccatact ggtggggatg cgagcgggta aggcttgcaa gctttcgcgt ttttcctgca
    65221 atccgtggcg ggctaaaaat gcgtcatcct ggagtaattt attcagcgta tcgcgatatt
    65281 gcgcgaattt cggatggtca gatgcgtcca tcatattgtc gatgaactgt tgcattggcg
    65341 gttgcttaat accttcgagg ttgtaaccga cgctcatatt aaagatgaac gatttaccgg
    65401 aatcagaagg ttggaacatc gcttcgagaa ggtgcagggc aaaccaggct ttgaggtatt
    65461 catcccaggc tttaagcagg gtaaattcgg tagaccattc ggtgttaaag cactcgtctt
    65521 cggcatcaat acagggcttt tccagctcca ggcggtcaag aatttggacg gtttttagtt
    65581 cgatgaaacg tccgccagtc agccaggaag tgacaatatt ttgcgcgagc tgcgtgtgcg
    65641 ggccagcggc agggccgacg ggagtggcac aggtttcacc gaagacgcta acagttttac
    65701 cttttacagg tgagtaaaac tgttgctcgg gaataccaaa gattgagcgt tgttgttggt
    65761 attcatcaaa tatgcgcgtc aaaagttcct caaacggaat gggacgcata atatccccca
    65821 taaccctctc cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg
    65881 tttaggggga tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa
    65941 aatgtgaggc ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga
    66001 gaattaaaat cactcctgaa aattattatt gccatcccat atatcacttt ttc
//
LOCUS       DAZFKY010000004        65450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFKY010000004 DAZFKY010000000
VERSION     DAZFKY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(147..629)
                     /gene="folK"
                     /locus_tag="ACHKCL_000231"
     CDS             complement(147..629)
                     /gene="folK"
                     /locus_tag="ACHKCL_000231"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5761276.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            complement(626..2023)
                     /gene="pcnB"
                     /locus_tag="ACHKCL_000232"
     CDS             complement(626..2023)
                     /gene="pcnB"
                     /locus_tag="ACHKCL_000232"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5761277.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2083..2979)
                     /gene="gluQRS"
                     /locus_tag="ACHKCL_000233"
     CDS             complement(2083..2979)
                     /gene="gluQRS"
                     /locus_tag="ACHKCL_000233"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5761278.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3046..3501)
                     /gene="dksA"
                     /locus_tag="ACHKCL_000234"
     CDS             complement(3046..3501)
                     /gene="dksA"
                     /locus_tag="ACHKCL_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5761279.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3679..4383)
                     /gene="sfsA"
                     /locus_tag="ACHKCL_000235"
     CDS             complement(3679..4383)
                     /gene="sfsA"
                     /locus_tag="ACHKCL_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5761280.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4398..4928)
                     /gene="thpR"
                     /locus_tag="ACHKCL_000236"
     CDS             complement(4398..4928)
                     /gene="thpR"
                     /locus_tag="ACHKCL_000236"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5761281.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            5002..7431
                     /gene="hrpB"
                     /locus_tag="ACHKCL_000237"
     CDS             5002..7431
                     /gene="hrpB"
                     /locus_tag="ACHKCL_000237"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5761282.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSCVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7627..10161
                     /gene="mrcB"
                     /locus_tag="ACHKCL_000238"
     CDS             7627..10161
                     /gene="mrcB"
                     /locus_tag="ACHKCL_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5761283.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10381..12624
                     /gene="fhuA"
                     /locus_tag="ACHKCL_000239"
     CDS             10381..12624
                     /gene="fhuA"
                     /locus_tag="ACHKCL_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5761284.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVELKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12675..13472
                     /gene="fhuC"
                     /locus_tag="ACHKCL_000240"
     CDS             12675..13472
                     /gene="fhuC"
                     /locus_tag="ACHKCL_000240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5761285.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13472..14362
                     /gene="fhuD"
                     /locus_tag="ACHKCL_000241"
     CDS             13472..14362
                     /gene="fhuD"
                     /locus_tag="ACHKCL_000241"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5761286.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14359..16341
                     /gene="fhuB"
                     /locus_tag="ACHKCL_000242"
     CDS             14359..16341
                     /gene="fhuB"
                     /locus_tag="ACHKCL_000242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5761287.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16376..17656)
                     /gene="hemL"
                     /locus_tag="ACHKCL_000243"
     CDS             complement(16376..17656)
                     /gene="hemL"
                     /locus_tag="ACHKCL_000243"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5761288.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17822..17869
                     /gene="yadX"
                     /locus_tag="ACHKCL_000244"
     CDS             17822..17869
                     /gene="yadX"
                     /locus_tag="ACHKCL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5761289.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17881..19302
                     /gene="clcA"
                     /locus_tag="ACHKCL_000245"
     CDS             17881..19302
                     /gene="clcA"
                     /locus_tag="ACHKCL_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5761290.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19326..19391
                     /gene="yadW"
                     /locus_tag="ACHKCL_000246"
     CDS             19326..19391
                     /gene="yadW"
                     /locus_tag="ACHKCL_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5761291.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19384..19728
                     /gene="erpA"
                     /locus_tag="ACHKCL_000247"
     CDS             19384..19728
                     /gene="erpA"
                     /locus_tag="ACHKCL_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5761292.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19775..20398)
                     /gene="yadS"
                     /locus_tag="ACHKCL_000248"
     CDS             complement(19775..20398)
                     /gene="yadS"
                     /locus_tag="ACHKCL_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5761293.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20436..21236)
                     /gene="btuF"
                     /locus_tag="ACHKCL_000249"
     CDS             complement(20436..21236)
                     /gene="btuF"
                     /locus_tag="ACHKCL_000249"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5761294.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21229..21927)
                     /gene="mtnN"
                     /locus_tag="ACHKCL_000250"
     CDS             complement(21229..21927)
                     /gene="mtnN"
                     /locus_tag="ACHKCL_000250"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5761295.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22011..23528
                     /gene="dgt"
                     /locus_tag="ACHKCL_000251"
     CDS             22011..23528
                     /gene="dgt"
                     /locus_tag="ACHKCL_000251"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5761296.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23658..25082
                     /gene="degP"
                     /locus_tag="ACHKCL_000252"
     CDS             23658..25082
                     /gene="degP"
                     /locus_tag="ACHKCL_000252"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5761297.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25237..26394
                     /gene="cdaR"
                     /locus_tag="ACHKCL_000253"
     CDS             25237..26394
                     /gene="cdaR"
                     /locus_tag="ACHKCL_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO5761298.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26483..26869)
                     /gene="yaeH"
                     /locus_tag="ACHKCL_000254"
     CDS             complement(26483..26869)
                     /gene="yaeH"
                     /locus_tag="ACHKCL_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5761299.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27184..28008)
                     /gene="dapD"
                     /locus_tag="ACHKCL_000255"
     CDS             complement(27184..28008)
                     /gene="dapD"
                     /locus_tag="ACHKCL_000255"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5761300.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28039..30711)
                     /gene="glnD"
                     /locus_tag="ACHKCL_000256"
     CDS             complement(28039..30711)
                     /gene="glnD"
                     /locus_tag="ACHKCL_000256"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5761301.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30773..31567)
                     /gene="map"
                     /locus_tag="ACHKCL_000257"
     CDS             complement(30773..31567)
                     /gene="map"
                     /locus_tag="ACHKCL_000257"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5761302.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31935..32660
                     /gene="rpsB"
                     /locus_tag="ACHKCL_000258"
     CDS             31935..32660
                     /gene="rpsB"
                     /locus_tag="ACHKCL_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5761303.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32918..33769
                     /gene="tsf"
                     /locus_tag="ACHKCL_000259"
     CDS             32918..33769
                     /gene="tsf"
                     /locus_tag="ACHKCL_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5761304.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33916..34641
                     /gene="pyrH"
                     /locus_tag="ACHKCL_000260"
     CDS             33916..34641
                     /gene="pyrH"
                     /locus_tag="ACHKCL_000260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5761305.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34791..35348
                     /gene="frr"
                     /locus_tag="ACHKCL_000261"
     CDS             34791..35348
                     /gene="frr"
                     /locus_tag="ACHKCL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5761306.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35440..36636
                     /gene="ispC"
                     /locus_tag="ACHKCL_000262"
     CDS             35440..36636
                     /gene="ispC"
                     /locus_tag="ACHKCL_000262"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5761307.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36825..37583
                     /gene="ispU"
                     /locus_tag="ACHKCL_000263"
                     /gene_synonym="uppS"
     CDS             36825..37583
                     /gene="ispU"
                     /locus_tag="ACHKCL_000263"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5761308.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37596..38453
                     /gene="cdsA"
                     /locus_tag="ACHKCL_000264"
     CDS             37596..38453
                     /gene="cdsA"
                     /locus_tag="ACHKCL_000264"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5761309.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38465..39817
                     /gene="rseP"
                     /locus_tag="ACHKCL_000265"
     CDS             38465..39817
                     /gene="rseP"
                     /locus_tag="ACHKCL_000265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5761310.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39847..42279
                     /gene="bamA"
                     /locus_tag="ACHKCL_000266"
     CDS             39847..42279
                     /gene="bamA"
                     /locus_tag="ACHKCL_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5761311.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42400..42885
                     /gene="skp"
                     /locus_tag="ACHKCL_000267"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42400..42885
                     /gene="skp"
                     /locus_tag="ACHKCL_000267"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5761312.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42889..43914
                     /gene="lpxD"
                     /locus_tag="ACHKCL_000268"
     CDS             42889..43914
                     /gene="lpxD"
                     /locus_tag="ACHKCL_000268"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5761313.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44019..44474
                     /gene="fabZ"
                     /locus_tag="ACHKCL_000269"
     CDS             44019..44474
                     /gene="fabZ"
                     /locus_tag="ACHKCL_000269"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5761314.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44478..45266
                     /gene="lpxA"
                     /locus_tag="ACHKCL_000270"
     CDS             44478..45266
                     /gene="lpxA"
                     /locus_tag="ACHKCL_000270"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5761315.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45266..46414
                     /gene="lpxB"
                     /locus_tag="ACHKCL_000271"
     CDS             45266..46414
                     /gene="lpxB"
                     /locus_tag="ACHKCL_000271"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5761316.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46411..47007
                     /gene="rnhB"
                     /locus_tag="ACHKCL_000272"
     CDS             46411..47007
                     /gene="rnhB"
                     /locus_tag="ACHKCL_000272"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5761317.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47044..50526
                     /gene="dnaE"
                     /locus_tag="ACHKCL_000273"
     CDS             47044..50526
                     /gene="dnaE"
                     /locus_tag="ACHKCL_000273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5761318.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50539..51498
                     /gene="accA"
                     /locus_tag="ACHKCL_000274"
     CDS             50539..51498
                     /gene="accA"
                     /locus_tag="ACHKCL_000274"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5761319.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51597..53738
                     /gene="ldcC"
                     /locus_tag="ACHKCL_000275"
     CDS             51597..53738
                     /gene="ldcC"
                     /locus_tag="ACHKCL_000275"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5761320.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53795..54184
                     /gene="yaeR"
                     /locus_tag="ACHKCL_000276"
     CDS             53795..54184
                     /gene="yaeR"
                     /locus_tag="ACHKCL_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5761321.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54249..55544
                     /gene="tilS"
                     /locus_tag="ACHKCL_000277"
     CDS             54249..55544
                     /gene="tilS"
                     /locus_tag="ACHKCL_000277"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5761322.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55597..55857)
                     /gene="rof"
                     /locus_tag="ACHKCL_000278"
     CDS             complement(55597..55857)
                     /gene="rof"
                     /locus_tag="ACHKCL_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5761323.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55844..56044)
                     /gene="yaeP"
                     /locus_tag="ACHKCL_000279"
     CDS             complement(55844..56044)
                     /gene="yaeP"
                     /locus_tag="ACHKCL_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5761324.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56210..56755
                     /gene="yaeQ"
                     /locus_tag="ACHKCL_000280"
                     /pseudo
     CDS             56210..56755
                     /gene="yaeQ"
                     /locus_tag="ACHKCL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
     gene            56752..57162
                     /gene="arfB"
                     /locus_tag="ACHKCL_000281"
     CDS             56752..57162
                     /gene="arfB"
                     /locus_tag="ACHKCL_000281"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5761325.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57176..57886
                     /gene="nlpE"
                     /locus_tag="ACHKCL_000282"
                     /gene_synonym="cutF"
     CDS             57176..57886
                     /gene="nlpE"
                     /locus_tag="ACHKCL_000282"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5761326.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58086..58910)
                     /gene="yaeF"
                     /locus_tag="ACHKCL_000283"
     CDS             complement(58086..58910)
                     /gene="yaeF"
                     /locus_tag="ACHKCL_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO5761327.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58963..60681)
                     /gene="proS"
                     /locus_tag="ACHKCL_000284"
     CDS             complement(58963..60681)
                     /gene="proS"
                     /locus_tag="ACHKCL_000284"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5761328.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60792..61499)
                     /gene="tsaA"
                     /locus_tag="ACHKCL_000285"
     CDS             complement(60792..61499)
                     /gene="tsaA"
                     /locus_tag="ACHKCL_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5761329.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61496..61900)
                     /gene="rcsF"
                     /locus_tag="ACHKCL_000286"
     CDS             complement(61496..61900)
                     /gene="rcsF"
                     /locus_tag="ACHKCL_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5761330.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(62018..62833)
                     /gene="metQ"
                     /locus_tag="ACHKCL_000287"
     CDS             complement(62018..62833)
                     /gene="metQ"
                     /locus_tag="ACHKCL_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5761331.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62873..63526)
                     /gene="metI"
                     /locus_tag="ACHKCL_000288"
     CDS             complement(62873..63526)
                     /gene="metI"
                     /locus_tag="ACHKCL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5761332.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63519..64550)
                     /gene="metN"
                     /locus_tag="ACHKCL_000289"
     CDS             complement(63519..64550)
                     /gene="metN"
                     /locus_tag="ACHKCL_000289"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5761333.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64738..65313
                     /gene="gmhB"
                     /locus_tag="ACHKCL_000290"
     CDS             64738..65313
                     /gene="gmhB"
                     /locus_tag="ACHKCL_000290"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5761334.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLSQAIKKQQKPAQ"
BASE COUNT        15450 a        16255 c        18060 g        15685 t
ORIGIN      
        1 ctaggttttt taaacaaaaa atgcctaatc tatttgcatg aagtgctatt tcatgaatta
       61 attaaaaaaa actaaaatgt aaattaagta tatttaatct gtaagagggg aagtgaggag
      121 taagataaga aaataaaata atacagctaa taccagatgg caggttgtag tactgctagc
      181 gaagtgagta tttcacgtaa tgtctcccca tcaggaaaca cgagttccgg ggcaatctca
      241 aacagcggcc acagcataaa gccgcgattc ttcatatcgt aatgcgggac ggtgagtcgt
      301 tcggtattaa tggtttcatt accaaacaac atgatatcaa gatcgagtgt gcgcggtccc
      361 cagcgttctg ctttacgaac gcgaccttgc tgcaactcta tttgctgtgt gtgattaagt
      421 aattcttcag gcgcaagtgt tgtttccagc gccacggctg cgtttaagta atcgggttga
      481 tcttgcggcc ccagcggtgg ggtgcggtaa aacgaagaaa cggtaagaat gtggctttca
      541 gggatatcgc ctaatgcttt cagggcagca ttgacctgct ccagcggaga ggccagattg
      601 ctgcctatgg caatatacgc cactgtcatg cggtaccctc acgacgtggt gcgcgtttgc
      661 gtggacgacg agtacgacga cgcggtgacg gttcttcatc cagctcgttg agcatccctt
      721 tttggtctgg tggcgcggaa acctggaact caccccacca tttcaccaga cgctgcagtt
      781 cagcgttacg ctcaacttca gctcgcaagg ccaacaggtc ataagccgca cggaacttag
      841 gatgctccag caatttccat gcgcgtttac cctgacgtcg ggacatacgc aactgcaact
      901 gccagatatc gcgggttaac gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt
      961 cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct
     1021 gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca aacaggaacg
     1081 ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc acctgttcaa
     1141 tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca
     1201 gcggctggaa cagatgatat tcacacaaca gcttataggt ttcgtaaccg tagcccgctt
     1261 gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc agcagggtag
     1321 cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat ttggcggcaa
     1381 aacgtactgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc gggttaccaa
     1441 tcagacggat aacaccgtcc ttcagatcct tcataccgcc aacgtaatca cggacggtaa
     1501 aatccgctac gctgtaatac aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt
     1561 cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg
     1621 tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg
     1681 ggccaaacat cacatgagcc agacggaaac ggcgacccac caggcggcag ttacggaaca
     1741 gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc ggctttttgc
     1801 cgagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc gctttattga
     1861 gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata gcatgctgct
     1921 cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc
     1981 ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac ctcggtagtg
     2041 tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg acgcatttga
     2101 gaatgttgaa tttacaattg ccgactcagg cactgcggta agccgccagt ttttgacggc
     2161 tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct gctgccccag
     2221 aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg
     2281 attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat gaatgtaatc
     2341 tggcactttc cagccaaaaa gctggtacag cgagatttgc cgcactgtcg gttcgatcaa
     2401 atctgccccg cggacaattt cgctcacgcc ctggaagtga tcatccacca ccaccgccag
     2461 gttataggca aacaaaccat cacggcgatg aataataaaa tcttcccgtg ccagtttttc
     2521 gtcggcgtga ataatgccgc gcagctggtc agtaaattgc gtgaccggat gctgctggcg
     2581 gatacgcact gcggcgttgt ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat
     2641 accgccaatg ctttgaatac gcgcacgcgt gcaggtgcag taataactta gtccttgttc
     2701 atgtaaccag gcgagtgctt cacgataggc gtggtgacgt tgcgattgcc agagaacatc
     2761 gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg cggcaccggg
     2821 aacttcacga ggcgggtcga tatcttcaat gcgtaccagc cagcgaccgt gccgggcgcg
     2881 agcctgcaaa tagctgccaa gcgcagcaat cagagaacca aaatgaagct cgccggaagg
     2941 agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca aaaaataagg
     3001 cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc cagccatctg
     3061 tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc
     3121 ttccagacgg cgaataccaa tttcaacacc gcaggattca cagtagccga aatcttcgtc
     3181 ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt
     3241 acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt ccgggaaatt
     3301 ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga gttgattacg
     3361 ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat actcttcgcc
     3421 cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg acgatgtttt
     3481 acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat ccccatgttc
     3541 gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa ttatctaaac
     3601 gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt
     3661 aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag ccatgccttc
     3721 agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag cttctgacaa
     3781 tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg
     3841 cagcacggcg aaaaagataa ccgcacgctg gccttcagcc gctacgctca tcaactcccg
     3901 aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc
     3961 taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct gcaacataaa
     4021 gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt ttcagcgagc tatagcctga
     4081 cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag cccaaagcgt
     4141 gttgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt gtgggtattt
     4201 ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg cacaaccggt
     4261 catcgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg tgatcacatc
     4321 ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc
     4381 cattgcgaca atccttgtta ttgcgttagc acccagcgtt ttagcggcgt gtagcgtgtg
     4441 cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata tgaccagtta
     4501 aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga
     4561 tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc
     4621 gccagctgga ttaagccgcg tggcggctga cgcatcccta accacaccac acgcgaacgc
     4681 agccattggc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct
     4741 aaaagagaaa gcgccttctc tttctctgcg ctcacttcgc ctaaaaatgc cagagtcaga
     4801 tgcaaattat cggcggcgac cggacgtccc gcctcaagtg ggaagtgtgt ggcgcgccag
     4861 tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag acgttgcggg
     4921 tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat
     4981 gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtgtt acctgaatta
     5041 cttaccgctc tcgattatgc gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa
     5101 tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa aattatcctc
     5161 ctggagccac ggcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac
     5221 gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat
     5281 acccgcctgg aagtagttac cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa
     5341 ctgagctgtg ttggattggt gatccttgat gaatttcatg agcgtagctt gcaggcggat
     5401 ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct taaactgctg
     5461 attatgtcgg ctacgctgga caacgaccgc ttgcagcaaa tgctgccaga agcgcccgtc
     5521 gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccgct gcccacgcat
     5581 cagcgttttg acgatgccgt tgcggtagcc accgctgaaa tgctgcgtca ggaaagcgga
     5641 tcattactgt tatttttacc tggcgtcgga gaaattcagc gtgtgcagga acaactggct
     5701 tcgcgcatcg gcagtgatgt attgctctgc ccgctgtatg gcgcgttgtc gctgaacgat
     5761 cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat
     5821 attgctgaaa ccagtttaac cattgaaggt attcgtctgg tggtggattg tgcccaggaa
     5881 agggtggcgc gttttgatcc gcgaacaggg cttacgcggt tgattactca gcgaatcagt
     5941 caggcgtcga tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg tatctgcctg
     6001 catttaatcg ccaaagaaca agcagaacgc gctaccgcgc aaagtgaacc ggagatctta
     6061 caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag cgatccggcg
     6121 cagatgagct ggctggatca accgccaacg gtgaatctac tggccgcgaa acgtctgtta
     6181 caaatgctgg gggcgctgga cggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg
     6241 ctgggtaacg atccgcgttt agcggcaatg ctggttagcg caaagagcga cgacgaagct
     6301 gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg caatagtgac
     6361 ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca gcaactgtta
     6421 aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc
     6481 ggggcgtttg ccgatcgcat tgctcgccgc cgtgggcaag atggacgcta tcaactggca
     6541 aacggcatgg gggcgatgct tgatgccgac gacgcgctaa gtcgccacga atggttgatc
     6601 gcgccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact ggcgttaccg
     6661 gtcgatattg atgaattagt acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg
     6721 gaatgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctgcaaat cggtcagttg
     6781 acggtgaaag tgcagccgtt ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt
     6841 aacggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc ggaacagcta
     6901 cgcttgcgtt tgttatgcgc cggaaagtgg ttgccagaat atgactggcc agcggttgat
     6961 gatgaaagtt tgttggcgac gctggaaacg tggctgctgc cacatatggc cggtgtacat
     7021 tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt acttgattgg
     7081 ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc gacgggaagc
     7141 cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag aatgcaggag
     7201 atgtttggtg aggccactaa tccgacgatc gcccaggggc gcgtgccgct ggtgctggaa
     7261 ttgctttcac ccgcccaaag gccgctgcaa atcacgcgtg atttgggggc cttctggaaa
     7321 ggggcgtatc gtgaggtgca aaaggagatg aaagggcgtt atcccaaaca cgtctggccg
     7381 gacgacccgg caaacacggc accgacgcga cggacgaaaa agtattcgta aggttggttt
     7441 tctccctctc cctgtgggag agggccgggg tgagggcacc agcgcgcacg ttcaccctca
     7501 ccctaaccct ctcccttaag ggagagggga ccgatcgagc tcaaattttg agagatatct
     7561 tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat attgcggaga
     7621 aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc gacgcgtccg
     7681 gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga cgattatgat
     7741 gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag
     7801 cctcgtggca aacgcggctg gctatggctg ctgttaaaac tggctatcgt ttttgccgtg
     7861 ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat tgatggcaag
     7921 gtctggcaac tgcctgcggc agtttatggt cgaatggtca accttgagcc agacatgacc
     7981 atcagcaaga acgagatggt gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa
     8041 atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat tcgccgtccg
     8101 tttgatttcc cggacagtaa agaagggcag gtgcgcgcgc gtctgacctt tgatggcgat
     8161 catctggcga ccatcgtcaa tatggagaac aaccgtcagt tcggtttctt ccgtcttgat
     8221 ccgcgtctga tcaccatgat ctcctcacca aacggtgagc agcgtttgtt tgtgccgcgc
     8281 agtggtttcc cggatttgct ggtggatact ttgctggcga cagaagaccg ccatttctac
     8341 gagcatgatg gaatcagtct ctactccatc ggacgtgcgg tgctggcaaa cctgaccgca
     8401 ggtcgcacgg tgcagggggc gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc
     8461 tccagcgagc gctcttattg gcgtaaagcg aatgaagcct acatggcgct gatcatggac
     8521 gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta tctcggtcag
     8581 agcggcgaca acgaaatccg cggcttcccg ctggcgagct tgtattactt tggtcgcccg
     8641 gtagaagaat tgagcctcga tcagcaggcg ctgttagtcg gtatggtgaa aggagcgtcc
     8701 atctacaacc cgtggcgtaa cccgaaactg gcgctggagc gacgtaatct ggtactgcgt
     8761 ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag tgcgcgtccg
     8821 ctgggggttc agccgcgcgg tggggtgatc tctcctcagc cagcctttat gcaactggtg
     8881 cgtcaggagc ttcaggctaa attaggcgat aaggtaaaag atctctccgg cgtgaagatc
     8941 ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt ggaaggtatt
     9001 ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac
     9061 cgctttagtg gtgaagttcg tgcgatggtc ggaggttctg agccgcagtt tgcgggctac
     9121 aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc gacttatctg
     9181 acggccttaa gccagccgaa aatctatcgt ctgaatacgt ggattgcgga tgcgccaatt
     9241 gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga atgatgaccg tcgttatagc
     9301 gaaagcggca gagtgatgct ggtggatgcg ttgacccgtt cgatgaacgt gccgacggta
     9361 aatctgggga tggcgctggg gctgcctgcg gttacggata cctggattaa actgggcgta
     9421 ccgaaagatc agttgcatcc ggttccggca atgctgctgg gggcgttgaa cttaacgcca
     9481 atcgaagtgg cgcaggcatt ccagaccatc gccagcggtg gtaaccgtgc accgctttct
     9541 gcgctgcgtt cggtaatcgc ggaagatggc aaagtgctgt atcagagctt cccgcaggcg
     9601 gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca gcaggtggta
     9661 caacgcggta cgggtcgtca gcttggggcg aaatacccga acctgcatct ggcagggaaa
     9721 acagggacta ccaacaataa cgtagatacc tggtttgcgg gcattgacgg cagcacggtg
     9781 accatcacct gggtcggccg tgataacaac cagccgacca aactgtatgg agccagcggg
     9841 gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct gaatcttgtt
     9901 ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt ttgcagcggt
     9961 ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca gcagagtgag
    10021 atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca gcaacagccg
    10081 caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg ttggattaag
    10141 gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag ttgccatccg
    10201 gtaaaataac atcccaccca aagtattaac cctttctttt catctggttg tttattaacc
    10261 cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagagtttcg aatattatct
    10321 tatctttata ataatcattc tcgtttacgt tatcattcac tttacatcag agatatacca
    10381 atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc agttgtagta
    10441 gccacagcgg ttagcggcat gtctgtttat gcacaggcag cggttgaact gaaagaagac
    10501 actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc tgctgcaact
    10561 attgcggcgc gacagtcagc taccggcact aaaaccgata cgccgattca aaaagtgcca
    10621 cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa
    10681 gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc caacacctat
    10741 gaccacctga tcattcgcgg ttttgcggca gaaggccaaa gccagaataa ctatctgaat
    10801 ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgatccgta tatgctggaa
    10861 cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag tcctggcggc
    10921 ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa
    10981 gccggtactg acagcctgtt ccagactggt tttgacttta gcgatgcgct ggatgatgac
    11041 ggcgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg
    11101 tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga tgataaaacc
    11161 aatttcacct tcctttctta cttccagaac gagccggaaa ccggttatta cggctggttg
    11221 ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac agactttaac
    11281 gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtgggcta cagcttcgat
    11341 cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga aaacaaaacc
    11401 tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa
    11461 cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa atacgtcgtt
    11521 gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa gtttgccact
    11581 ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg taatgacatc
    11641 aacgcctggt ttggttacga cgactccgta ccgctgctcg atctgtacaa tccggtgaat
    11701 accgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg cattctgaat
    11761 aagcagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa agtgctggtc
    11821 accctgggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt tgccgggacg
    11881 accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta cctgtttgat
    11941 aatggcgtaa caccttactt tagctatagc gaatcgtttg aaccttcttc gcaagttggg
    12001 aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat
    12061 gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac taaaaccaac
    12121 aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga gatccgcgca
    12181 cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct
    12241 tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa tacgcctgca
    12301 caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga cggtccgctt
    12361 tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg tgatccggct
    12421 aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg
    12481 cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa
    12541 tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt
    12601 gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggatttccg tgcctgtttc
    12661 acaagttggc tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta
    12721 atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg
    12781 ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact ctgctcaaaa
    12841 tgcttggccg tcatcagccg ccgtcggaag gggagattct tcttgatgcc cagccgctgg
    12901 aaagctggag cagcaaagcg ttcgcccgca aagtggctta tttgccgcag cagcttcctc
    12961 cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg
    13021 cactagggcg ctttggtgcg gcagatcgcg aaaaagttga ggaagctatc tcgctggttg
    13081 gcttaaaacc gctggcgcat cggctggtag acagcctctc tggcggcgaa cgtcagcggg
    13141 cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgactt
    13201 cggcgttgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg
    13261 agcgtggcct gacggtcatt gccgtgctgc acgatatcaa tatggctgcg cgttactgtg
    13321 attatctggt cgccctgcgc ggcggtgaaa tgattgctca gggaacgcct gcggaaatta
    13381 tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattctg ccgcatccgg
    13441 cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg
    13501 tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc
    13561 ggcggctatt gacccaaatc gtattgtggc gctggagtgg ctgccggtgg aattactact
    13621 ggcgctcggc atcgtgcctt atggcgtggc agacaccatc aactatcgac tgtgggtcag
    13681 cgaaccacca ctacctgaat cggtgattga cgtcggtttg cgtacagaac ctaacctcga
    13741 actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcgggatatg gtccttcgcc
    13801 agaaatgctg gctcgtattg cgccggggcg tggatttaac ttcagcgacg gcaaacagcc
    13861 gctggcgatg gcgcgtaaat cgctgacgga aatggcggat ttacttaacc tgcaaagcgc
    13921 agcagaaacg catttagcgc aatatgaaga ctttatccgc agcatgaacc cccgctttgt
    13981 gaagcgtggt gcgcgtccgt tattgctgac gacgcttatc gatccgcgcc atatgctggt
    14041 cttcggtcca aacagcttgt tccaggaaat tcttgatgag tacggcatcc caaatgcctg
    14101 gcaaggggaa accaacttct ggggcagtac cgccgtcagc atcgatcgtc tggcagcgta
    14161 taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat
    14221 ggcaacgccg ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc agcgcgtacc
    14281 cgcagtctgg ttttatggtg caactctctc ggcaatgcac tttgtgcgcg ttctggataa
    14341 cgccattgga ggtaaagcgt gagtaaacgt attgcgcttt tcccggcatt attgctggcg
    14401 ttgctggtga ttgccgctac ggcgctgacc tggatgaact tctcgcaggc gctgccgcgt
    14461 agccagtggg cgcaggctgc ctggtcgccg gatattgacg tcatcgagca gatgattttt
    14521 cactacagct tgctgccgcg tctggcgatt tcgctgctgg tgggcgcagg cctggggctg
    14581 gtgggcgtgc tgtttcagca agtgctgcgt aatccgctgg cggaaccgac gacacttggc
    14641 gttgctacag gcgcgcaact ggggattacc gtcactacgc tctgggcgat cccaggggcg
    14701 atggcgagtc agtttgctgc gctggcgggc gcttgcgttg ttggcttaat cgtctttggc
    14761 gtcgcgtggg ggaaacggct ttcgccggta acgctgatcc tcgcggggct ggtagtgagc
    14821 ctttattgtg gggcaatcaa tcagttactg gttatcttcc accatgacca actgcaaagc
    14881 atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggtgt tgagcgttta
    14941 tggccgcagt tgctgggcgg tgtgatgctg acgttgctgc tacttcgtcc gttaacactg
    15001 atggggcttg atgatggcgt ggcgcgcaat ctcgggctag ccttgtcgct ggcgcgtctg
    15061 gcggcgctgt cgctggctat tgtcatcagt gcgctgctgg tgaacgcggt ggggattatc
    15121 ggctttatcg ggttgttcgc gccgttgctg gcaaaaatgc tgggggcgcg gcgtctgctg
    15181 ccgcgactga tgctggcgtc gttgattggt gcgctgatcc tctggctttc cgatcaaatc
    15241 atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc
    15301 ggtgcgccgc tgctactgtg gctgttgccg cgtttacgca gcattagcgc gccggatatg
    15361 aaggtcaacg atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc
    15421 ggcgtgctgc tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc
    15481 tggacgtggg cgagcggggc gttgctcgag gatttgatgc cctggcgctg gccgcgaatt
    15541 atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcggctg
    15601 accggaaacc cgatggcaag cccggaagtg ctggggatta gctctggcgc ggcgtttggc
    15661 gtggtactga tgctgtttct ggtaccgggt aatgcttttg gctggctgtt acccgcggga
    15721 agtatcggtg cggcggtgac gctgttgatt attatgatcg ccgccgggcg cggtggattt
    15781 tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcattcac catgctttta
    15841 atgatgttgc aggcgagtgg tgacccgcga atggcgcaag tgctgacctg gatctctggt
    15901 tcgacctaca acgccaccga tgcgcaggtc tggcgcaccg gaattgtgat ggtgattttg
    15961 ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt taccgctggg tggtgatacc
    16021 gcgcgggccg tgggggtggc gttaacgccg acgcgaattg cgctgctgct gttagcggct
    16081 tgcctgacgg cgaccgcgac gatgaccatt ggaccgttga gttttgttgg tttaatggca
    16141 ccgcatatcg cgcggatgat gggctttcga cggacgatgc cgcacatcgt gatttcggcg
    16201 ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt gctgtttccg
    16261 ttccagatcc cggcggggct gctgtcgacc tttatcggcg cgccatattt tatctatttg
    16321 ttgagaaagc agagccgtta ataaaatgcc tcgcccggtg ggcgaggcaa aatcatcaca
    16381 acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc atgctgtgcg
    16441 ccacagacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct tcatccagca
    16501 tcatatggaa gaaacgctta aaacgctcga cgtcacaggc catcacatcc tgatagcacg
    16561 tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg tttaccacca
    16621 gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt gttgtcagct
    16681 catccagcgt ttcgtgaacg cccggctgcg cgacttcatt cagacaggcg aaaccggctg
    16741 ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggaccc gtcggggcca
    16801 gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca ccgccgatga
    16861 ttttgcccag gcaagtgaga tccggttcca caccgtaata atcctgtgcg ccagctagcg
    16921 ctacgcggaa accggtcatt acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca
    16981 gcgcgcgcag acctggcagg aagtctggca gcggtggaac acagttcata ttgcctgcca
    17041 ccggctcgac gataatacag gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag
    17101 aagccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc ggaacgcccg
    17161 gcgagtttgg ctggcctaac gtgagtgcgc cagagccggc tttcaccagc aggcagtcag
    17221 cgtgaccgtg gtaacaacct tcaaatttaa taattttgtc gcgaccggta aaaccacggg
    17281 ccaggcggat ggcgctcatt gtcgcttcag tgccggagtt caccatgcgc accatatcca
    17341 tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg gttggtgcac
    17401 caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc ggatggttat
    17461 ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg ccatcaacat
    17521 cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc acgccagtaa
    17581 aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat
    17641 tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc tgctattcta
    17701 tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact ggtgcaatcc
    17761 tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc aaaggatttt
    17821 catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag tgatcattgg
    17881 atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa
    17941 ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt tatggcggca
    18001 gtcgtcggca cgcttgtcgg gctggcagcg gttgcttttg acaaaggtgt cgcctggttg
    18061 cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct actgttaacc
    18121 gttgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt gcgcaaatac
    18181 gcgcccgaag caggggggtc ggggatcccg gaaattgaag gggcgctgga agatcaacgt
    18241 cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg gacactcggc
    18301 ggaggcatgg tgttggggcg cgaagggcca accgtgcaga tcggcggtaa tatcgggcgg
    18361 atggtactcg atgttttccg cctgaaaggt gatgaagctc gccatacgct gttggcaacc
    18421 ggtgcggcgg cgggtctggc ggcggccttt aacgcgccgc tggcgggtat tttgtttatt
    18481 atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc ggtatttatt
    18541 ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc gttgattgac
    18601 gtcggtaaac tttctgacgc gccgcttaat acgctgtggc tttatctgat cctcggtatt
    18661 atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca ggatttgctg
    18721 caccgtgtgc acggtggcaa tattaccaaa tgggtactga tgggtggtgc gattggcggt
    18781 ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt taacctgatt
    18841 cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt cgtcgcgcgg
    18901 gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg
    18961 ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga gctgtttccg
    19021 caatatcacc ttgaggcggg gacgtttgct attgccggaa tgggggcgtt actggcggca
    19081 tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga taactaccag
    19141 ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca atttaccggc
    19201 gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga agctgagcaa
    19261 ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata ccagggtatt
    19321 agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg tttggagcaa
    19381 aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc caacaaagtt
    19441 aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta tatcaccggt
    19501 ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa cgaaggcgat
    19561 atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct gcaatatctg
    19621 gtcggcggtt ccgttgacta taccgaagga ctggaaggtt ctcgtttcat cgtgaccaac
    19681 ccgaacgcga aaagcacctg cggttgcggt tcttccttta gtatctaatc tgttgttcat
    19741 gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca tccagcgcaa
    19801 acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc gtcacgacca
    19861 tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc gtagcgtgga
    19921 caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc atggggattt
    19981 cgcgggccag aacatcacga atgatcccgc cgccaacgcc agtaatgacg cccatacaaa
    20041 ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg ccgacaaaca
    20101 ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagtgt cttggctggc
    20161 gcaccagcac gatggtcagc atgctggtta ccattgcaac gaccagatcg gtgggatctt
    20221 tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgtcccg ccgcctacag
    20281 cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt ccggctaaca
    20341 aaacgccgga gatggcaaat accgctgtgc cgactatatc cagccaatag acgagcattg
    20401 ttcaatcccc actgagcacc taaaaaggtc aggcgctaat ctacctgtga aagcgcatta
    20461 cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca gtcactcgtg
    20521 agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg
    20581 tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg ttcgcggcta
    20641 acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt
    20701 acctggtttt gaatcgactc ttttccactg gtaaatggcg gattaatgcc gaattgcaga
    20761 aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta ttgatccagc
    20821 agggattgcg cggcttgttc ggccttatcc ggttgcggac tccagggagc cagttgacgt
    20881 aacgcattgg caatttgttc aatgtttgtc gcatcgaccc acatcacttt tattcccagc
    20941 gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg
    21001 ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatc
    21061 ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg
    21121 gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc
    21181 cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt agccatgtgc
    21241 cagtttctgc actagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa
    21301 ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc gtacgacaac
    21361 aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc
    21421 aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt tgatgaaagc
    21481 gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa tgcaggcctc
    21541 agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc
    21601 gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac
    21661 gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt taataatcac
    21721 atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga ctttaccgat
    21781 gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc
    21841 gcccagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc
    21901 cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaggca
    21961 tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga
    22021 ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa
    22081 ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg
    22141 caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat gccgccgtgc
    22201 gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc gccaaagaaa
    22261 ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat gaactgaccg
    22321 gaccttttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc aatccgccgt
    22381 ttggtcattt tggcgaagcg gcgataaatg actggtttcg ccagcgtttg cacccggaag
    22441 atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggtacta cgtttacggg
    22501 acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta tgtcattttg
    22561 aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat ctcacctggg
    22621 cgcaggttgg cggtatttta aaatataccc gtccagcgtg gtggcgtggc gaaacgcctg
    22681 agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa gcctatattg
    22741 cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta
    22801 tggaagctgc cgacgacatc tcctattgtg tggcagatct tgaagatgcg gtagagaaaa
    22861 gaatatttac cgttgagcag ctttatcatc atttgcacga ggcgtggggc cagcatgaga
    22921 aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt
    22981 taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac
    23041 tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt
    23101 ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag ctatataaaa
    23161 atgtcgctgt aaaacatgtg tttagccatc cagatgtcga gcagcttgaa ttgcagggct
    23221 atcgggtcat tagcggatta ttagagattt atcgcccttt attaagcctg tcattatcag
    23281 actttactga attggtagaa aaagaacggg tgaaacgttc ccctattgaa tctcgcttat
    23341 tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt
    23401 cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt
    23461 atatcagcgg tatgaccgac ctctatgcct gggatgaata ccgacgtctg atggccgtag
    23521 aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta
    23581 gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt
    23641 aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt
    23701 taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag
    23761 cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca
    23821 ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt
    23881 tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct
    23941 gccagggtgg ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt
    24001 ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata
    24061 acgcgacggt cattaaagtt caactgagcg atggccgtaa gttcgacgcg aagatggttg
    24121 gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaatccgaaa aacctgaccg
    24181 caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcgattggta
    24241 acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg
    24301 gcctgaatgc cgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta
    24361 actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc
    24421 tagcaccgga cggcggtaac atcggtatcg gttttgctat cccgagtaac atggtgaaaa
    24481 acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg
    24541 ggactgagct gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt
    24601 tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg
    24661 tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt gctcaagtgg
    24721 gtactatgcc ggtgggcagc aaactgaccc tgggcttgct gcgcgacggt aagcaggtca
    24781 acgtgaatct ggaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca
    24841 acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca
    24901 acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg
    24961 gcgcgaacca gcaggctgtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac
    25021 cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt
    25081 aatctccctc aaccccttcc tgaaaacggg aagggggtct ccttacaatc tgtgaacttc
    25141 accacaactc catacatctt catcatcctt taggcatttg cacaatgccg tacgttacgt
    25201 acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc
    25261 aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa tatcaacgta
    25321 atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac
    25381 gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca
    25441 cgtcatctgc acggtgtgcg gcaagggatt aatctaccgt tacggctgga aggtgaaatt
    25501 gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc
    25561 tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt ggcgcaggat
    25621 agccgtttgc gggaggaact ggtgatgaac ctgattcagg cagaggagaa tactcccgca
    25681 cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt
    25741 gttgaggtcg acagcggtca gcttggtgtg gacagcgcaa tggcggagtt acaacaactg
    25801 caaaacgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa
    25861 atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgtaga agatcatcgt
    25921 aagcgagttg aacaactgat tacccgcatg aaagagtacg gccagctgcg ttttcgcgtt
    25981 tcactgggca actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa
    26041 acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt ttatcaggat
    26101 ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg
    26161 cgaccgctgg cgcggctgaa agcgatggac aataacggct tgctgcgacg aacgctggcg
    26221 gcgtggtttc gtcacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt tattcatcgt
    26281 aataccctgg agtatcggct taatcgtata tcggaactga ccgggcttga tttgggcaat
    26341 tttgatgaca ggttgctgct gtatgtggcg ttacaactgg atgaagagcg gtaggttatg
    26401 cgttaaggtt gaggcggcgc tggctcattc ccctcagccc gaaggggaga ggggaatgcc
    26461 cgagttaacc gtcaattcgg gattatttac gcgttagttt ttccagatcg gcttcaatct
    26521 cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg atcttacgct
    26581 taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca atgatatacc
    26641 gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca tcagcgacga
    26701 ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac tcgcctttgt
    26761 ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg ctgtaacgat
    26821 caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata gcgttacctc
    26881 aaaatgagtc agtaaaatgt gcttatttta gcatttggcc tggcccgccc ggctcgactg
    26941 tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc aaaaaattac
    27001 ctgctttatt gtggcgatat tccatgggat ggattagcac atttatcttc gttttgcgaa
    27061 ttacatgaca gtccgaaaca agatgaaaaa gtatttaaaa aaggggggag gaagaatgag
    27121 taggacgaat tattctgaac cagaaagcaa tcgggcagcg tcgtgctgcc cgtgtgcata
    27181 cttttagtcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc
    27241 aactttctta acgataaccg cacagtagag gctgtatttg ccatctttcg acggcagatt
    27301 acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatgtcgc cggtttcacg
    27361 gtcgtaaata cgggtgctct gaccaatgta tacgcccatg gaaatgacgg aaccttcttc
    27421 gacaatcacc ccttcaacta cttcagagcg cgcgccgatg aagcaattat cttcaatgat
    27481 ggttgggtta gcctgcagcg gttccagcac accgccgatg cccacgccac cggaaaggtg
    27541 gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcga ccatggtgcc
    27601 ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt tacgggcaat
    27661 aaacgcaccc tgacgtaccg ccgcaggtgg tacaacgcgg aagccttctt tctggaaacg
    27721 tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg aagtagcggc tttctgcccc
    27781 ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg
    27841 atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag
    27901 ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct gcattggctg gcgtgatctc
    27961 ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt
    28021 ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg
    28081 atgcacttcc tgctgcaact cgttattaag tgcacgccgg tcagcggtgg caattatgaa
    28141 taaatcttct actcgctcgc caatggttgt aattcgggca ccatgaagcg aaattcccag
    28201 atcggcaaaa attttcccga ctctcgccag cagtcctggc tggtcgagcg cgataagctc
    28261 aaggaacgat ttgcggtcgg tatgggttgg caaaaacgtc acttcggttt caacagtaaa
    28321 gtgacgtagt ttagccggtt gacgacgcgg ctgtggtggc tgccagctac tttgtgtcag
    28381 tacttgctcc agaccaaacc gaataacctc atgacgatct gcggatagcg ggctaccatc
    28441 gggttccagc acgataaagg tatccatcgc cataccgtcg cgagtggtga aaatttgtgc
    28501 gtcgtgaacg cttaagttgc gtctatctaa ctcggcacaa acagcagcaa acaagtaagg
    28561 gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg ggctaagcaa
    28621 taccagcggt ttgcttaaat catgctgtaa taaatggcgc gcatgccagg ccagttgatt
    28681 tgggctatga cggacaaaat agttcgcgcg acagcgtgac caaatttggt gcagcacctc
    28741 ttcgtcgatg ttatccatgc gcagtagtgc cagcgcctgg agttggtgat ggcgaacccg
    28801 ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcgaaata
    28861 gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg tggcgcaaat
    28921 gtcagccact gtcaggcata ccagatagcg cagacgattt tccgtttgaa cttcttcggc
    28981 aaactgcttg atgacttccg ggtcctgaat atcgcgacgc tgagctgtca ccgacatcag
    29041 caagtgctgg cgaaccagcc aggcgaccag ctgtgtttcg cgtgagttca gcccgtggag
    29101 ttcggcaaaa tgcactacat cctgagcacc gagaatagag tggtcgccgc cgcgtccttt
    29161 ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggta ggcgcggcca
    29221 gacgttcaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt
    29281 cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat caaactgcat
    29341 ctgcccgacg atatgtgacc attgcggcat atacgcgccg agtacgctat ggcgatgcat
    29401 tggcaatagc ccgcgccgca ccgctccggg gtgacgtaga atgctcaaaa acagttttcg
    29461 tgcttgcgga atattacaca gcggttgttg cagatggcga cgggcatggc gtaactggcg
    29521 cagtgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg
    29581 caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga ttagcgtacc
    29641 gcgtagctga aactcatcgt cgattggacg tggtttttcg tcggcgggaa gggcgaggat
    29701 ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc gtgtcacgcg
    29761 gaagtaatcc ttcatcatcc gctcgaccgg ttcgttacct tcgccactgt aattcagacg
    29821 ctgggcgacg ctaagctggc gatcgaataa caggcgatta tcgtaacggc tgacgaccag
    29881 atgcagggca aagcgaatac gccacaatat atgcagacat tcgtttaatt ccgcccgctc
    29941 cgctgaggtt aagaagccaa acccaaccat ttcatccagc gatgttgcgc caaaatgacg
    30001 gcgggccacc cattgcagag tgtggatatc gcgcaagccg ccagggctac ttttgatgtc
    30061 tggttcaaga ttgtagctgg tgccgtggta acgctgatgg cgctggttct gttcttcaac
    30121 tttcgccgcg tagaacttgt cggaaggcca gaatccttcg ctgaaaatat gtttttgcag
    30181 ttcaagaaat agcgcaacat cgccaattaa taagcgggat tcgatcaaat tggtggcgac
    30241 ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacac tatgaccgac
    30301 ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcgtc
    30361 cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat gcagctcgcc
    30421 acgaccgtag ccaccgacgg cgaccaatgc caggtcggca atctggctga atcccgcttc
    30481 aatccataat cgttgcagga gctggtcgat aaactcggtg cgcgcctcaa tcaactgttc
    30541 tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc
    30601 ttttatccca ccgacggtta attcatcacg gggccagacg catggatttt gcggttgacc
    30661 gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca
    30721 cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca ttttattcgt
    30781 cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt tcgcagccgt
    30841 tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta
    30901 ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcga
    30961 tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca tagtgcagca
    31021 cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca cgaacgacgg
    31081 agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc agattaatgc
    31141 ctggttttac catgcgtagc gccaggtaca ggctttcttg cgtgatacgg cacagacgtt
    31201 cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat
    31261 ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc ttagcatcgt
    31321 ccggaatacc gtggcacacc acttcattaa tagaaatgca aacggatttc ggataaccgt
    31381 gatagccgag gcaggctgaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc
    31441 gatccagctc gccggtgctg acgcccggtt taacatatgg ttcgatcatc tccagcactt
    31501 cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga
    31561 tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc
    31621 aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga
    31681 aggttttcta taatagaaaa ttcgacgtct gatgctgtac acagcgccaa caattattgg
    31741 tgtccacggc gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca
    31801 gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc
    31861 cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata
    31921 tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact
    31981 tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta
    32041 acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg
    32101 aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg
    32161 caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct
    32221 ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag
    32281 acttggaaac tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga
    32341 tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg
    32401 gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagaag
    32461 caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg
    32521 ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg
    32581 ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa
    32641 gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc gagttgcaga
    32701 aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg gatgagcgag
    32761 cgaagatgac gcatctgcgg cgcgaaatat gaagggggag agcccttata gaccaggtag
    32821 tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata tctgtgcggt
    32881 ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta ccgcatccct
    32941 ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac
    33001 tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa
    33061 agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg
    33121 caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg
    33181 tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt
    33241 tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga
    33301 aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca
    33361 tggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca
    33421 catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccggaag acgtatccgc
    33481 tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc
    33541 gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc
    33601 tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga
    33661 gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca tcgagaaagt
    33721 tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat tatcaaaaag
    33781 gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta acccttgtgg
    33841 ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag
    33901 tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat tctgcttaag
    33961 ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag catactggat
    34021 cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg tgtggtgatt
    34081 ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg
    34141 ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg tgatgcactg
    34201 caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg cgtgtgcgac
    34261 agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc
    34321 gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg tggtatcgaa
    34381 attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac cgctgatccg
    34441 gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt gctggaaaaa
    34501 gagctgaaag tcatggacct ggcggccttc acgctggctc gtgaccataa attaccgatt
    34561 cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa
    34621 gggactttaa tcacggaata attcccgtga tggataaata agggtaagat tccgcgtaag
    34681 tatcgcgggg gatcttaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34741 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34801 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34861 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    34921 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    34981 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35041 cgtccgatct tggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35101 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35161 cgcgtgttgc agtacgtaac gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35221 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35281 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35341 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35401 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35461 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35521 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35581 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35641 atgctacagc aacagggtag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35701 atggcagcgc ttgaggatgt tgatcaggtg atggcagcca ttgttggcgc tgctgggctg
    35761 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35821 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    35881 ccggtcgata gcgaacataa cgccattttt cagagtttac cgcaacctat ccagcataat
    35941 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36001 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcctgc
    36061 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36121 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36181 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36241 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36301 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36361 gccgcaccgg attatgatcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36421 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36481 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36541 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgag cgcgcgtgaa
    36601 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36661 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    36721 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36781 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36841 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    36901 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccggag
    36961 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37021 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37081 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37141 tgcgtattat tggcgatacc agtcgcttta actcgcgttt gcaagaacgt attcgtaaat
    37201 ctgaagcgct aacagccggg aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37261 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37321 tgcaaccaga tcagatagat gaagagatgc taaaccagca tgtctgtatg catgaactgg
    37381 cccctgtaga tttagtaatt aggactgggg gggagcatcg cattagtaac tttttgcttt
    37441 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37501 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37561 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37621 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37681 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37741 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37801 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37861 tttgggcttc gctgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    37921 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    37981 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38041 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38101 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38161 cctggcaagg ctttatcggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38221 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38281 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38341 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38401 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38461 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38521 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38581 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38641 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38701 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    38761 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38821 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    38881 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    38941 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39001 cattacagta gcgccatttg gcagcgacca acggcgggat gtaaagctcg atttacgtca
    39061 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39121 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39181 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39241 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39301 ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39361 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39421 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39481 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39541 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39601 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39661 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaaag gcggaccggt
    39721 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39781 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39841 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    39901 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    39961 gttggtgcgg ccctcctcag tatgccggtg cgcacaggcg acacggttaa tgatgaagat
    40021 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40081 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40141 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40201 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40261 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40321 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40381 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40441 ctgcgtgacg aagtgccgtg gtggaacgtg gtaggcgatc gtaaatacca gaaacagaaa
    40501 ctggcgggcg accttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40561 aacatagact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40621 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40681 gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct gtataacggc
    40741 accaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40801 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40861 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    40921 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggctggga
    40981 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41041 gataccgata cccaacgtgt tccgggtagc ccggaccagg tagatgtagt ctacaaggtg
    41101 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41161 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41221 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41281 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41341 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41401 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41461 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    41521 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41581 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41641 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41701 gtgccgatcg atgacgatca caaatgggtt gttctggggc gtacccgctg gggttatggt
    41761 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41821 accgtgcgtg gcttccagtc caatacaatt ggtccgaaag cagtttactt cccgcatcag
    41881 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    41941 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42001 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttccttcttc
    42061 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42121 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42181 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42241 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42301 agtggtagtg tagcgatgac tttaggcgat caatataaat cgccgggcca cgcaaagaac
    42361 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42421 gcaggtctcg gtttagcact ggcaacatct gctcaggcgg ctgacaaaat tgcaatcgtc
    42481 aacatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42541 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42601 atgaaaaagc tgcagtccat gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg
    42661 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42721 cgttccaatg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42781 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42841 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    42901 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    42961 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43021 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43081 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43141 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43201 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43261 gaatccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43321 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43381 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43441 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43501 gatcgcgtgg agatcggtgc ctgcactacc atcgatcgcg gcgcgctgga tgacactgtt
    43561 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43621 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43681 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43741 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43801 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43861 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    43921 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    43981 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44041 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44101 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44161 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44221 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44281 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44341 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44401 aaggggttgc tctggtcgac ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44461 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44521 aagagggcgc gtcgattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44581 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44641 aaattggccg cgataatgag atttatcagt tcgcctccat cggcgaagtt aaccaggatc
    44701 tgaaatatgc tggcgaaccg acccgcgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44761 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44821 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    44881 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    44941 gcatggccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45001 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gccacgccgt
    45061 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45121 tccgcaatgc gtataagctg atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45181 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45241 gctcaacgcg cggtctgatt cgttaatgac tgaacagcgt ccattaacga ttgccctggt
    45301 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45361 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45421 agcctggtac gaaatggaag agttggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45481 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggtgaac taaagccaga
    45541 tgtttttgtt ggcattgatg cgcctgactt caatattatc ctcgaaggta accttaaaaa
    45601 gcagggtatc aaaaccattc attatgtcag tccgtccgtc tgggcgtggc gacagaaacg
    45661 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45721 gttttatgac aaatacaacg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45781 gccattagat ccagataaaa atagtgcccg tgatgtgctg gggatccctt acgatgccca
    45841 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    45901 cctgaaaacg gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccact
    45961 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgcagtcg cgccagacct
    46021 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46081 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46141 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46201 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46261 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaatggga aaaccagcca
    46321 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46381 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtctatcc gcacacgcag
    46441 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46501 gcggtgattc tcgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46561 gaaaaacgtc gtctggcgct ctgtgaagag atcaaagaga aagcgttgag ctggagtctg
    46621 ggccgcgcgg aacctcacga aatcgacgaa ctgaatattt tacatgcgac catgctggcg
    46681 atgcagcgag ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46741 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg
    46801 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46861 gatattgttt tcccgcaata tggttttgcc caacacaaag ggtatccaac cgcttttcat
    46921 ctggaaaaac tggctgaaca cggcgcgacc gaacaccatc ggcgcagctt tgggcctgtc
    46981 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47041 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47101 ggcctggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47161 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47221 gcagggatta agcctatcgt cggggcagat tttaacgtcc agtgcgacct gctgggtgat
    47281 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctgacgttg
    47341 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47401 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47461 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47521 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47581 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47641 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47701 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47761 atgcgtagcg aagaggagat gtgcgagctg tttgccgaca tcccagaagc ccttgccaac
    47821 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    47881 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagagggc
    47941 ctggaagagc gtctggcctt tttattccct gatgaggaag aacgtgttaa gcgccgcccg
    48001 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48061 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48121 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48181 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48241 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtagcg
    48301 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48361 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgatcgtatc
    48421 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48481 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgttgatcga catggcgcgc
    48541 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48601 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48661 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48721 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48781 ggcgagccgc cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48841 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    48901 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    48961 cctggtccgt tgcaatcagg gatggtggat aactttatcg accgtaaaca tggtcgcgaa
    49021 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaaccggt actggagcca
    49081 acctacggca ttatcctgta tcaggaacag gtcatgcaga ttgcgcaggt gctttctggt
    49141 tataccctcg gtggcgcgga tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag
    49201 atggctaagc aacgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49261 ctggcgatga aaatcttcga cctggtggag aaattcgctg gttacggatt taacaaatcg
    49321 cactctgcgg cctatgcttt ggtgtcatat caaacattat ggttgaaagc gcactatccg
    49381 gcagagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49441 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49501 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgcgatcaaa
    49561 ggggtaggtg aaggtccgat tgaggcgatc atcgaagccc gtaataaagg cggctacttc
    49621 cgcgaactgt ttgatctctg cgcccgaacc gacaccaaaa agttgaaccg tcgcgtgctg
    49681 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacatcgcgc ggcgctgatg
    49741 aactcgctgg gcgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49801 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctatgcc
    49861 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    49921 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    49981 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50041 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50101 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50161 catttgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50221 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50281 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50341 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50401 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50461 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50521 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50581 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50641 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50701 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccaca gcgtccttat
    50761 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgcgcg
    50821 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    50881 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    50941 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51001 atgcctatca tcacctttat cgacaccccg ggggcttatc cgggcgtggg cgcagaagaa
    51061 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct cggcgtaccg
    51121 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51181 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51241 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51301 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51361 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51421 gccgatctcg acgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51481 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51541 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51601 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51661 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51721 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgaat
    51781 acagtctcga tttatgtagc gatatcaatc agcttaatga atatctcccg ctttatgcct
    51841 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctgtt
    51901 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacaccg
    51961 acgaatatct tgataacatt accccaccgt tcacgaaagc cttgtttacc tacgtcaaag
    52021 agcggaagta caccttttgt acgccgggtc atatgggcgg caccgcatat caaaaaagcc
    52081 cggttggctg tctgttttat gactttttcg gcgggaatac ccttaaggct gatgtctcta
    52141 tttcggtcac cgagcttggt tcgttgctcg accacaccgg gccacacctg gaagcggaag
    52201 agtacatcgc gcggactttc ggcgcggaac agagttatat cgttaccaac ggaacatcga
    52261 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgattgacc
    52321 gcaattgtca taaatcgctg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52381 tgaaaccgac gcgtaatgcg ttggggattc tgggtgggat cccgcgccgt gaatttactc
    52441 gcgacagcat cgaagagaaa gtcgctgcca ccacgcaggc acaatggccg gttcatgcgg
    52501 tgattaccaa ctcaacctat gatggcttgc tctacaacac cgactggatc aaacagacgc
    52561 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtacacccat tttcatccga
    52621 tctaccaggg gaaaagtggt atgagcggcg agcgtgttgc gggaaaagtg atcttcgaaa
    52681 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcgctgatc cacattaaag
    52741 gtgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52801 gttatcccat tgttgcttcg gttgagacgg cagcagcgat gctgcgtggt aatccgggca
    52861 aacggctgat taaccgttca gtagaacgag ctctgcattt tcgcaaagag gtccagcggc
    52921 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    52981 ccgaatgctg gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53041 atcatatgtt cctcgatccg gttaaagtca ctattctgac gccggggatg gacgagcagg
    53101 gcaatatgag cgaggagggg atcccggcgg cgctggtggc gaagttcctc gatgagcgtg
    53161 ggatcgtggt agagaaaacc ggcccatata acctgctgtt tctctttagt attggcatcg
    53221 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaaacgc tcttacgatc
    53281 tcaatctgcg aatcaaaaat atgcttccgg atctgtacgc agaagatccc gatttctacc
    53341 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53401 ttcccggttt gatgttgcgg gcattcgata ctttgccgga gatgatcatg acgccacatc
    53461 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53521 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgcctg
    53581 gagaaatgct gaccaaagag agccgcacag tactcgattt tctactgatg ctttgttccg
    53641 tagggcaaca ttaccccggt tttgaaacgg atattcacgg tgcgaaacag gacgaagacg
    53701 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53761 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53821 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    53881 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    53941 gcttaatggg caatatgtga ttgagctttt ctcatttccg ttcccgccgg agcgccccag
    54001 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54061 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54121 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54181 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54241 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54301 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54361 aaaatccggg gggcgctctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54421 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54481 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcgcgtt
    54541 atcaggcatt tgcccgcacc ttgttgcccg gtgaagtgct ggtcaccgcg caacatctcg
    54601 acgatcaatg tgaaactttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54661 cggctatggc ggaagtttcg gagtttgccg gaacacggct tatccgcccg ttgcttgccc
    54721 gcacgcgggg ggaactggcg cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54781 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54841 tgcagcagcg ttggccgcat tttgccgaaa caacggcccg cagcgccgca ctttgtgctg
    54901 aacaagagag cctgctggat gaactgctgg cagatgattt agcacactgt caatcgccgc
    54961 aggggacgct gcagattgtg ccaatgctgg cgatgagtga tgcccgccgc gcggcgatta
    55021 tccgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ttggtgagga
    55081 tctggcagga agtggcgctg gcgcgggaag atgcctcccc ctgtttacgt ttgggcgcgt
    55141 ttgaaatccg acgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55201 aaaacattgt gccgtggcag acatggcttc aaccgctgga attaccggcg gggcagggaa
    55261 gtgtacagct taatgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55321 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg taacggcgga cgtaagctaa
    55381 agaaaatctg gcaagagctg ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55441 tttatggcga aacgctgatt gcggcggcag ggggatttgt gacgcaagaa ggtgtggctg
    55501 aaggtgagaa tggcataagt tttgtctggc agcgaaacgc ttagtttagt gaaggccgga
    55561 tatgacgcat taagcgtcgc atccggcgaa agtcaatcag gactcgctca ccaccaccgt
    55621 accgatttcc gggtggctaa agctggtaat tttatccaga cgtagctcac gggtttcgcc
    55681 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcgc tggctttggc
    55741 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    55801 ctcaagatta tcgtaatcat cacaattgat tggttgatac gtatcattca ttgacataat
    55861 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    55921 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    55981 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56041 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56101 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    56161 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56221 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56281 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgcct
    56341 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    56401 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    56461 ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct
    56521 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgc
    56581 gcagtttgcc aatctttccg tctggtatct ggacgacgag cagctggcga aagtgagcgc
    56641 ctttgccgat cgtaccatga cgctgtaggc aacgattcag gatggcgtga tctggttatc
    56701 ggatgataag aataatctgg aagtgaactt aaccgcctgg caacaacctt catgattgtg
    56761 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    56821 ggcgcgggcg ggcagcatgt taataagacc tcaacggcca ttcatctgcg ttttgacatt
    56881 cgggcatcca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg
    56941 atcagcagtg atggcgtaat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57001 cgcgaagcgg cgctggcccg gctggtggca gtgattaaag atttaacaac agaacaaaaa
    57061 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57121 caaaaatcaa gcgtgaaggc gatgcgcagc ggtcgggaat aaaaagaagg aatggatggt
    57181 gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga tgggttgtaa
    57241 taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaattga aaccgatgcc
    57301 gcaaagttgg cgcggcgtgc tgccgtgtgc cgattgcgaa ggaatcgaaa cctctctgtt
    57361 cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc gtgaagaacc
    57421 ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg tattaaccga
    57481 cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga tgctcgatcg
    57541 tgaaggcaat ccgattgaat cgcagttcaa ctatacgctg gaaccggcac aatccagttt
    57601 acccatgacg ccgatgaccc tgcggggcat gtatttttat atggctgatg cggcgacctt
    57661 cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc tggagcgtgg
    57721 ctacctggct gcgcgcggcc acagtgaaaa accggtgtta ctgtcagtag aaggtcactt
    57781 tacgcttgag gctaatccgg ataccggtgc gccaaccaaa gtattagcac ccgatacggc
    57841 agggaaattt tacccaaacc aggattgcag tagtttgggg cagtaacccg tcttgagaca
    57901 gaaacaaacg caaaacaggc cagaaggata tatttcaaca ttttgaattt gcacgttttt
    57961 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatgg tgctcaacgc ctgatgcgac
    58021 gctggcgcgt cttatcatgc ctacaacccc cctcatacct taaggcggat aaggcattta
    58081 ctttatcacc gggtcaaacc aacaaagcga cccgccttga tataaatccc tggcttcagg
    58141 tgcccaacat actgtaactg cgtttctggc ttaaacgcag agacatcacc aatacgcata
    58201 tgcatcagat cggcagggct aatccagccc gattgcgcca gcgtaagcgg atgcccggct
    58261 ttggcaaatg catcagtgac aaattccgaa caaaaccacg actttttgtc tccttcgacc
    58321 acactgctta actgcgcttt cgccaggccg ctgacgcact gttggcgaaa atcctcggag
    58381 aacggattca gtgagcacat ctggcgagtc accataaagg gaataaattc gacaatgccg
    58441 cgatagttat aaccgctatc tttgatttta ttggcgaacg cggtgatttc tgtggcttgt
    58501 tgcggggtaa gatccgggac tcgtaagacg aaaagtttat cactatgctt catcgctttt
    58561 ttaagggaaa caatctggac gccagcgcct gtcgcttctg caacgttatt ttcacccaga
    58621 tagattgcaa cgtgactcac agaggaagtg ctgaaaacgc ggatcccaaa tgaggttacc
    58681 tccaggcttg aggagaacag caaatcgccg ggtttgagat caggtaccgt tatttctttt
    58741 attgattgtt cggtgaaaga gctttgatgc tggaatttga cagcccatgt tttcgcctcg
    58801 gcatctactg ctgtggctga tgagtctggc tggctgatat caacggtaca ggccgaaagt
    58861 aaaagaaagc aggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat
    58921 ccatgtaaaa aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt
    58981 caccagatat tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata
    59041 tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat
    59101 accgatcagt tccatatcgg caaacatcac gcccggacgt tcttttcggt catccagcag
    59161 cacttcgata ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg
    59221 gaaggatttg tgcatgttca tcggcagaat tgccacctgg aacggcgcga tagcgtcagg
    59281 ccatacgatg ccgcgctcgt cgtagttctg ctcaatcgcc gcagccacta cgcgcgttac
    59341 cccgataccg tagcaaccca tagtcaggat ttggttacgg ccatcttcac cctgtacgga
    59401 ggctttcagt gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc
    59461 acgtttgatc agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg
    59521 gatatccgca acttccgggg tagcgacatc gcgatcccag ttgatgccga agtagtgttt
    59581 accatcgatg ttagcaccag cagtgaaatc actcatcgcc gcaacggtac ggtcaatcac
    59641 caccggaatc ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacggcacg
    59701 aatttcttct tcggtcgcga aagtcagcgg gctggcaacc tgcggcagtt tttctgcttt
    59761 aacttcgttc agctcgtgat caccgcgcac cagcagcgca accagcggga agctactgcc
    59821 ttcaaccgct ttaaccagca gagtcttaac cgttttctta atcggcagat tgaactgttc
    59881 aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc
    59941 agcagcgcgc ggttctttcg gcgcgatagc ttctgccagc tcaatgttcg ctgcatagtc
    60001 agaggtgtcg gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg
    60061 agaggcgctg ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat
    60121 gcggctgaag attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc
    60181 ctgagaagta tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc
    60241 gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag
    60301 ctgtttgtaa gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg
    60361 gccgagtacg aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg
    60421 ttcccaacga ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc
    60481 gattgcaccg gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg
    60541 cacgccggtc ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg
    60601 cagcatcagc tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag
    60661 caggtattgg ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag
    60721 gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga
    60781 agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc
    60841 cagcgaacgt taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta
    60901 cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga
    60961 taacgcttct ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc
    61021 tctgcagctg gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag
    61081 gggagatacg gtttgatatc cactaccggc gtaccatcga ccagatccag gctgccgagc
    61141 ttcagaatca cgcagtcttt atggcaaaca acctctttca gctctaccag cgacatacca
    61201 atcgggttgg ggcggaaagt ggagcgcgta gcgaaaacac ccattctggc attaccgcca
    61261 agacgaggcg gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa
    61321 aggatccata aatggctgaa tgcttccagg ccgcgaacgg cgtcagcctg gttgtaggga
    61381 gcaatgagat gtagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg
    61441 aatttttctt tatagggcga gcgaataaca cctatttgct cgaactggaa actgctcatt
    61501 tcgccgtaat gttaagcgca gaaccgatac ataaagcctg acgatagcag cctggcgtac
    61561 cgctggtaac ttcgcagcta tgcagtaata ccgcattggc tttcattttc gaggcgttga
    61621 tttgcatccg cttacgcgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc
    61681 aagagtcgcc actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat
    61741 tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg
    61801 gctggggtgc agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc
    61861 ttagcatgag tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga
    61921 tcaaaacgtc aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc
    61981 ctgaatgata ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc
    62041 cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc tggacgaatt
    62101 tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtacgggg
    62161 agtctttatc ttcgacaaag ataccgtttt tcgctggagt caggccaatc tggctggcat
    62221 aggtggtgtt aataactgcc agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg
    62281 gtgcttccag ttcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg
    62341 gcagcaggcc aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg
    62401 aacgaccaag gttagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat
    62461 ccagtgattt gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg
    62521 cgaccagttt gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg
    62581 cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg
    62641 ttaccagctc aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcaa
    62701 cctgctgttc ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct
    62761 gaccgcagcc taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga
    62821 atttgaacgc cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc
    62881 gagtgacagc ccggacgatg cggtcgcctg cgaactgaat taaataaacc agaatgacca
    62941 gcaataccag taccgtattc atcaccgtcg cgttatagcc gatgtagcca tactgatagc
    63001 caatctgacc taaaccaccg gcaccgactg caccacccat cgcggaataa ccgaccaggg
    63061 taatcagggt gatagttgcc gcattcacca gacccggcaa cgcttccggt aacagcactt
    63121 tacggacgat ctgcattggc gtggcaccca ttgcgcggga agcttcaatt aacccggttg
    63181 ggatctccag cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta
    63241 acggaacaat tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa
    63301 tcatccatac cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa
    63361 cggtacgata cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagga
    63421 cgccaaccgg cagaccaacc acaaacccaa aaaatccgga tacgaaggtc attgccagcg
    63481 tttcccatac gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct
    63541 acttttacat ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg
    63601 tgcatttcag tcagcatgat gccgaactta acgccaccgg cgtaatccat ctgcgcgcta
    63661 ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc
    63721 gattgaccgg taaactccag acgcagcatc ggcacgcagt cagtaaatgg ctccgcttgc
    63781 agacgttcct ggtaatcttc cgggatatcc agatgcaggg ttgactgaat aaacttctgc
    63841 gccagcggcg ttttcggatg tgagaacact tcacttaccg tgtcctgctc gatcagttcg
    63901 ccattgctga tgaccgcaac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg
    63961 atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc gagaatagaa
    64021 cgtgtcgttg ccgggtctag cgcgctggtg gcttcatcac acagcaatac tttgggattg
    64081 ctggctaatg cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg
    64141 tagctatcat gcttatctcc aagaccaacc aatgacagca attccgtcac gcgacgtttg
    64201 atctcgtctt tcggtgtatt gtccagctcc agcggcagag ccacgttgcc aaaaacagta
    64261 cgcgaagaga gcaggttaaa atgctggaaa atcataccaa tctggcggcg agctttggtc
    64321 aactcggatt ctgacagcgt ggtcagttcc tgtccatcga ccagcacgct accctcggtt
    64381 gggcgctcca gcaggtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg
    64441 ataacgccat aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg
    64501 gtgcgggtgc cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta
    64561 ttatcgtcat taagttagtc gtggcatctc gaatgcctga aacgggcaac gccgtcaatg
    64621 aaatggatgt taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc
    64681 ttgcaggtcg aaacatgcga tactagcgtc acatgcctta ttaaggagct ataaaaggtg
    64741 gcgaagagcg tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc
    64801 tatgtccatg agatcgacaa ctttgaattt atcgacggtg ttattgacgc catgcgcgag
    64861 ctaaaaaaaa tgggctttgc gctggtggta gtcaccaacc agtctggcat tgctcgcggt
    64921 aaatttaccg aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac
    64981 cgagatgtcg atctggatgg tatctattat tgcccgcatc atccgcaggg tagtgttgaa
    65041 gagtttcgcc aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc
    65101 gattatttgc atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg
    65161 caggcagcgg ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt
    65221 acacctgaag cagaaaacgc ggcagattgg gtgttaaata gcctggcaga cctgtcgcaa
    65281 gcgataaaaa agcagcaaaa accggcgcaa tgattaaaag atgagcggtt gaaataaaaa
    65341 tgcatttttc cgcttgtctt cctgagccga ctccctataa tgcgcctcca tcgacacggc
    65401 gaatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaaat
//
LOCUS       DAZFKY010000005        54310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFKY010000005 DAZFKY010000000
VERSION     DAZFKY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..2301)
                     /gene="pbpC"
                     /locus_tag="ACHKCL_000291"
     CDS             complement(<1..2301)
                     /gene="pbpC"
                     /locus_tag="ACHKCL_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5761335.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHF"
     gene            complement(2302..7263)
                     /gene="yfhM"
                     /locus_tag="ACHKCL_000292"
     CDS             complement(2302..7263)
                     /gene="yfhM"
                     /locus_tag="ACHKCL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5761336.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7470..8315
                     /gene="sseA"
                     /locus_tag="ACHKCL_000293"
     CDS             7470..8315
                     /gene="sseA"
                     /locus_tag="ACHKCL_000293"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5761337.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8658..8783
                     /gene="timP"
                     /locus_tag="ACHKCL_000294"
     CDS             8658..8783
                     /gene="timP"
                     /locus_tag="ACHKCL_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5761338.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8815..9591)
                     /gene="sseB"
                     /locus_tag="ACHKCL_000295"
     CDS             complement(8815..9591)
                     /gene="sseB"
                     /locus_tag="ACHKCL_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5761339.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRALLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9734..11017)
                     /gene="pepB"
                     /locus_tag="ACHKCL_000296"
     CDS             complement(9734..11017)
                     /gene="pepB"
                     /locus_tag="ACHKCL_000296"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5761340.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11195..11395)
                     /gene="iscX"
                     /locus_tag="ACHKCL_000297"
     CDS             complement(11195..11395)
                     /gene="iscX"
                     /locus_tag="ACHKCL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5761341.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11407..11742)
                     /gene="fdx"
                     /locus_tag="ACHKCL_000298"
     CDS             complement(11407..11742)
                     /gene="fdx"
                     /locus_tag="ACHKCL_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5761342.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11744..13594)
                     /gene="hscA"
                     /locus_tag="ACHKCL_000299"
     CDS             complement(11744..13594)
                     /gene="hscA"
                     /locus_tag="ACHKCL_000299"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5761343.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13611..14126)
                     /gene="hscB"
                     /locus_tag="ACHKCL_000300"
     CDS             complement(13611..14126)
                     /gene="hscB"
                     /locus_tag="ACHKCL_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5761344.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14222..14545)
                     /gene="iscA"
                     /locus_tag="ACHKCL_000301"
     CDS             complement(14222..14545)
                     /gene="iscA"
                     /locus_tag="ACHKCL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5761345.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14562..14948)
                     /gene="iscU"
                     /locus_tag="ACHKCL_000302"
     CDS             complement(14562..14948)
                     /gene="iscU"
                     /locus_tag="ACHKCL_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5761346.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14976..16190)
                     /gene="iscS"
                     /locus_tag="ACHKCL_000303"
     CDS             complement(14976..16190)
                     /gene="iscS"
                     /locus_tag="ACHKCL_000303"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5761347.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16302..16790)
                     /gene="iscR"
                     /locus_tag="ACHKCL_000304"
     CDS             complement(16302..16790)
                     /gene="iscR"
                     /locus_tag="ACHKCL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5761348.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17091..17828)
                     /gene="trmJ"
                     /locus_tag="ACHKCL_000305"
     CDS             complement(17091..17828)
                     /gene="trmJ"
                     /locus_tag="ACHKCL_000305"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5761349.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17947..18750
                     /gene="suhB"
                     /locus_tag="ACHKCL_000306"
     CDS             17947..18750
                     /gene="suhB"
                     /locus_tag="ACHKCL_000306"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5761350.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18895..19749
                     /locus_tag="ACHKCL_000307"
     CDS             18895..19749
                     /locus_tag="ACHKCL_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5761351.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19940..21220
                     /gene="csiE"
                     /locus_tag="ACHKCL_000308"
     CDS             19940..21220
                     /gene="csiE"
                     /locus_tag="ACHKCL_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5761352.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21212..22351)
                     /locus_tag="ACHKCL_000309"
     CDS             complement(21212..22351)
                     /locus_tag="ACHKCL_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5761353.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22511..23401)
                     /gene="hcaR"
                     /locus_tag="ACHKCL_000310"
     CDS             complement(22511..23401)
                     /gene="hcaR"
                     /locus_tag="ACHKCL_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO5761354.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23537..24898
                     /gene="hcaE"
                     /locus_tag="ACHKCL_000311"
     CDS             23537..24898
                     /gene="hcaE"
                     /locus_tag="ACHKCL_000311"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO5761355.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24895..25413
                     /gene="hcaF"
                     /locus_tag="ACHKCL_000312"
     CDS             24895..25413
                     /gene="hcaF"
                     /locus_tag="ACHKCL_000312"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO5761356.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25413..25733
                     /locus_tag="ACHKCL_000313"
     CDS             25413..25733
                     /locus_tag="ACHKCL_000313"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO5761357.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25730..26542
                     /gene="hcaB"
                     /locus_tag="ACHKCL_000314"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25730..26542
                     /gene="hcaB"
                     /locus_tag="ACHKCL_000314"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO5761358.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26552..27754
                     /gene="hcaD"
                     /locus_tag="ACHKCL_000315"
     CDS             26552..27754
                     /gene="hcaD"
                     /locus_tag="ACHKCL_000315"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO5761359.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27851..28273
                     /locus_tag="ACHKCL_000316"
     CDS             27851..28273
                     /locus_tag="ACHKCL_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5761360.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28321..29193)
                     /locus_tag="ACHKCL_000317"
     CDS             complement(28321..29193)
                     /locus_tag="ACHKCL_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5761361.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29205..30299)
                     /locus_tag="ACHKCL_000318"
                     /pseudo
     CDS             complement(29205..30299)
                     /locus_tag="ACHKCL_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30332..31330)
                     /locus_tag="ACHKCL_000319"
     CDS             complement(30332..31330)
                     /locus_tag="ACHKCL_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5761362.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31355..32866)
                     /locus_tag="ACHKCL_000320"
     CDS             complement(31355..32866)
                     /locus_tag="ACHKCL_000320"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5761363.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32889..33872)
                     /locus_tag="ACHKCL_000321"
     CDS             complement(32889..33872)
                     /locus_tag="ACHKCL_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5761364.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33969..37250)
                     /locus_tag="ACHKCL_000322"
     CDS             complement(33969..37250)
                     /locus_tag="ACHKCL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5761365.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDIAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37368..38561
                     /locus_tag="ACHKCL_000323"
     CDS             37368..38561
                     /locus_tag="ACHKCL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5761366.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38624..39877)
                     /gene="glyA"
                     /locus_tag="ACHKCL_000324"
     CDS             complement(38624..39877)
                     /gene="glyA"
                     /locus_tag="ACHKCL_000324"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5761367.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40205..41395
                     /gene="hmpA"
                     /locus_tag="ACHKCL_000325"
     CDS             40205..41395
                     /gene="hmpA"
                     /locus_tag="ACHKCL_000325"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5761368.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41440..41778)
                     /gene="glnB"
                     /locus_tag="ACHKCL_000326"
     CDS             complement(41440..41778)
                     /gene="glnB"
                     /locus_tag="ACHKCL_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5761369.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41839..43173)
                     /gene="glrR"
                     /locus_tag="ACHKCL_000327"
     CDS             complement(41839..43173)
                     /gene="glrR"
                     /locus_tag="ACHKCL_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5761370.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43163..43876)
                     /gene="qseG"
                     /locus_tag="ACHKCL_000328"
     CDS             complement(43163..43876)
                     /gene="qseG"
                     /locus_tag="ACHKCL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5761371.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44041..45468)
                     /gene="qseE"
                     /locus_tag="ACHKCL_000329"
     CDS             complement(44041..45468)
                     /gene="qseE"
                     /locus_tag="ACHKCL_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5761372.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46044..49931)
                     /gene="purL"
                     /locus_tag="ACHKCL_000330"
                     /gene_synonym="purI"
     CDS             complement(46044..49931)
                     /gene="purL"
                     /locus_tag="ACHKCL_000330"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5761373.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50188..51744
                     /gene="mltF"
                     /locus_tag="ACHKCL_000331"
     CDS             50188..51744
                     /gene="mltF"
                     /locus_tag="ACHKCL_000331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5761374.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51741..52244)
                     /gene="tadA"
                     /locus_tag="ACHKCL_000332"
     CDS             complement(51741..52244)
                     /gene="tadA"
                     /locus_tag="ACHKCL_000332"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5761375.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52302..52937)
                     /gene="yfhb"
                     /locus_tag="ACHKCL_000333"
     CDS             complement(52302..52937)
                     /gene="yfhb"
                     /locus_tag="ACHKCL_000333"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5761376.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53146..53994
                     /locus_tag="ACHKCL_000334"
     CDS             53146..53994
                     /locus_tag="ACHKCL_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5761377.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54050..54310
                     /gene="yfhL"
                     /locus_tag="ACHKCL_000335"
     CDS             54050..54310
                     /gene="yfhL"
                     /locus_tag="ACHKCL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5761378.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        12803 a        15029 c        13744 g        12734 t
ORIGIN      
        1 aaaatgtact gacgcgattt gccctacgtc atccatcacc agcaattgat aatcaccttt
       61 atccgtcaaa tgcagggtca cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa
      121 ccaccagcgt tcacctgccc ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc
      181 cggtaaacgt ttaataatct cgccatcgcg aacgcctgtc agctgcagcg ggagctgggc
      241 atcgtgaccg tatggcgggc aaattgtcga ggccggtggt aagcgcacag cgcgacgttc
      301 tgatgcgggc aaccagggtt ccagcggtag cggccagaca tttatcattt cttgtcgcgc
      361 ttgcgggcaa tcagcggcaa cacgtttgcc attttcatcc agccagatgg ggaaacgaat
      421 gccattaatg ccttcctgct ccggcagtaa tagagttggc ggctgactcc cgtccagcag
      481 ccaggttgcc aggcggcggc gacagttacc gtcaccttcc ggcaaagact gtccgcccgg
      541 ccagcaaata acgccacgag tgactgagtt cgggcgcggg tcttccggca gattagcact
      601 gcgcgacagt aagatattat tgacctgatt caacaatggt acggcgctgg caaaaccaaa
      661 ctgaccaaca acgggcgtgc cgtccggtct gccagtccag atcccaatga catagcgagc
      721 gttaacccca atcgcccagg cgtcacgata gccatagctg gtgcccgttt tccatgccag
      781 tggggcgacg cgcggcaagg caccatccgg caagggtagc gcttcatcag ccataatccg
      841 gcgaatgatc cacgccgctc ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg
      901 ctgtaagcgc aatttgcctg ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat
      961 atcttccagt ttcgcaccag cgccgccgag aatgagcgaa agattcggcg cagcaccgtt
     1021 gggcaaatat aacggcaatc caacattgcg taactttgcc gcaaaccgtt tcggtccata
     1081 ggcttccagc acctgcacag caggtaaatt cagcgagcgc accagcgcct cgctcatgct
     1141 gatcgggcca tgaaaaccgc tgtcaaagtt gccaggtcgg taatcaccgg tacgccgggg
     1201 gacgtcttgc agcagtgatg ccgggtggat caatccttca tccagcgcca gaccgtaaac
     1261 aaacggtttg agcaccgatc ctggcgatcg gatcgcattg accatatcaa catgaccaaa
     1321 gcgtgaatca tcgttgagat caaccgaacc cacccagccg cgaacacgca tatcggtatg
     1381 atcaaccacg atcatcgcca gtgagctgcg cggtggtaat cgtcctttcc agttttgcgc
     1441 cagttcttcc agacgtcgtt gaagaccggc atccagcgta gtaacgattt tgtcgctttt
     1501 gcttttaccg agcatcatgc gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc
     1561 cagccagatg ggttcttccc ttgactcttt tacctgctca cgggaccaca caccttgcac
     1621 ggccatccgt tcgagcactt tattacgcgc gacttcggca cgctccggcc aacgatccgg
     1681 gcgaagacgg ctgggcgctt gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa
     1741 attcgcaggc gactttccga gataagccca acttgccgca ccgatcccct gcaacgtacc
     1801 gccaaacgga gcgcggttaa gatacaaggt cagaatttca cgcttagaca gatgccattc
     1861 cagttgcaac gcgcgccaga gctggcgaat tttgccgcca aatgttttgg ggtgaggatc
     1921 aagcagacga gcaacctgca tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt
     1981 gagatcttgc catgctgcgc gcgccaccga gaatggattc accccaggat gcttccagaa
     2041 ccagcgatct tcataattga tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt
     2101 tacaggataa cgccagatgc cgtcagcatc ggcgaagcgc cagagcggcg taccatcctg
     2161 cgccacgacc actcgtgcgg gattgacttc atgcagcggt agaggccaga gtttatctgc
     2221 tccccacgct gcaagaaaaa ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg
     2281 tttggttaac aagcgaggca tttacggtct gacaatcagc aaatcttcag ccgcgccggt
     2341 cgcccgccat tggggaacat acattgattc caccatcggt tgcggtacct gatacgttcc
     2401 cggcgtcacc gcccgcgcca gatacaccag cgttaccggt tggtattcat caacggcaac
     2461 cgccgccaca aagcgatcgt cacggaactc aatgtgctta atactcgcct gctgcatctg
     2521 gttcagtaag ttctgcactt cgccaccact ttgctccagg ctggcgctac cgttcgccag
     2581 attctggttt tccagttcca gacccgcagg cagcagatcc acgactaacg catccggcac
     2641 gctgttacta gcttttacct gcaaccagac cagcaccaga tcgccgctac gtaacgagtc
     2701 cagcgatttg ctcttaccat cagtaccaag aatatgacgc tcaatttgca gcacattgtt
     2761 cgccggtaaa ggtgcggatt gcggataacc gctggcatcc acacgcagcc ataacggctg
     2821 ctcgccactg ttggtcacct gcaaggtggc aagttgatcg ctattcagat tgctgttttg
     2881 cgctttctcg cctgtcagcg gctcagctga gaaagaggtt tgcgcctgcc atttacctgg
     2941 taaatcctga atcgtacggg cagccaggaa caacgcgtta ctttcctgcg tcgatagcca
     3001 gcgttcacca aacgcctgct gcgaaagtgt gttcagcagg gagtactgct catcgggtag
     3061 cagtttgttc tcttccagca aggagagcat caacgcgttg tcgcgcagtg gactaccgta
     3121 atcacccagc catatccgct catcactatt acgcggcgtt tttagcgcca gcgcaatcgc
     3181 ttcttcatca cgcgtcgcat cacccatggt tttcagcgca acgccaagtt gcagcagcgg
     3241 taaaccagaa gcggcatctg cacgatgctc ccagatttca cgcagcgcac ccagcggagc
     3301 cttttgctga cgggccaaca ccagcgcagc gtaagactgt acggcgaatt tactggcttt
     3361 gagattatcc gcgtacggga tcgacatcat gcccggatct tgtaaatagc gcagcagacg
     3421 ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac
     3481 caggaaatcc atcacgtaag ccgtcagcca gtactcttcg tcaccgtttt tatcccacag
     3541 cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg gaaatgccga tatcgaccga
     3601 tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt
     3661 ggtataaagt gacgggaaca ggccgctggc ggtttgctca agacagccgt acggatacgc
     3721 ttttaactct ttgatataac gtgcgatgtt cagcggtggt ttgccgctca acaacaattg
     3781 cccttccagc gtaacaggcg agaagttttg caatccatcc gccggaatcg cccatgtctc
     3841 accaggctgt aacgccgtac cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc
     3901 gattttccac tgcttatgct gatcggcaac ggtttcaccc ggtaacgcta acccgctaat
     3961 ggtggcctga atttctccat cgccataacc cggcaatgct cgcaccggga taaacagcgt
     4021 agtacgcaca cctggcgcta attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa
     4081 accactggcg gtcagggcaa cgttcagttt ttgcggttta tcggtaagat tagtgatatc
     4141 cagcgtcaga cgcgagttat cgccactcgc cataaagcgc ggcatgttca gttcagcaat
     4201 caccggtgcg gcaactatca ctttgctttc gttgctaccg aattcatctg ccgtccaggc
     4261 ttgcgccatg acgcgcaatt caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc
     4321 gccctgttcg ttgagcgtta ccggcagcgc ctgctgcgcg acaatattga cgtgattgac
     4381 cggcggttta ccaccacgtt tcagctcatc accatcgcca ccgaaacgca gagctgccag
     4441 acgcccctga ccttcaataa cctgaccgta aatatcgtaa atgtctgcgc catagcgttt
     4501 ctgaccaaag aacgcctgcc acggatctgg cgtgacgtag tcagtaatat tcagcacacc
     4561 actatcaacg gcagacacca gcacattcac ctgtttaggc ttctcgccat ttttagtgct
     4621 ggctttaatt ttcacggtta atggttgatt gggacgcatt tttgctggtg tttccagcgc
     4681 cagatcgagg cgacggtttt catcaccaag cggcagatgc aacacaccaa ccgcgcgttt
     4741 tggcgtcgcg gagcgagatt tatcgccagg acgcaccacc agcgtactca aataaagatc
     4801 atgacgattc caggttttat cgaccggaat cgtcagatcc agcccttgag ccggaacatc
     4861 aatctcttgc caccacagcg gcccttcact ggactcgacc atcgcataac ctttacccgc
     4921 cgttggcgcg gcgatatgca acttaatggt atcgccaggg cgataaccgg ctttatccag
     4981 tttcagcgtg acacggtcgg gtcgcactgc gccgctaccg tcgctattat cctgccagct
     5041 atagccagcc caaaaacgaa cactactgac cgcttcattc ggcgctttga cttccagacg
     5101 ataagcgccc cactctaccg ggaaactgac cttgccggtt tcgtccgctt tcagatccag
     5161 agtttgttca ttttcaatca gatctttttg atcaaactgt gattgccagc cttcatcttc
     5221 tgaccagttc cagtagtaat cgcggcgttc gcgaatcagg cgcacctgca agcccgacac
     5281 ggcttttttc acgccttgcg catcgctata aacgatgtca aaagcggcgt tactgccttc
     5341 atcaacaatg ggctgtttca ccgtgctgtc agtgcgataa tcgtaaaccg atttcgaggc
     5401 gaactgcgga cggatccccg gcaatgcatc ggcaggccag atagcctgct cagcgcggcg
     5461 cgtcaccggg cgaccgcccg attccagcaa actgccctgg aaaataacct gcaatgggga
     5521 atgcgtttcc ttccactggc tttctgtaga aacttcgccg cgccctttat catccagcgt
     5581 caactgaact tcatccagcg tgcgggaaag gttttcggca gctatatcgc cgaattcaaa
     5641 accaggtaag gctgacacag cctcacgcag tgggcgcagg aaaagttgcc cttgcaaagt
     5701 attaccatta gcaggcgcac cgtacaggta gtatcccacc acggagaatt tcacttcatc
     5761 attcggcgta agcggggttt tctcaccggt cagattcagc gccatacgct ctggcataaa
     5821 atcttcgacg tggaaatccc acatccgata ctggttatcg cccgtgttag cgcgaatatg
     5881 ccacataccg gttgccgcat tgctatcgag tggccaggta aagtggtaga ggccattttc
     5941 cggctgacta acgacgctcc tgagtacctg cccatcgggt ttaatcacgt ctaacttgat
     6001 gggttgattg ggcaacgctt taccgtctgc atcacgcagc aaaccattga ggattaccgt
     6061 ttcacccggg cgataaagat cgcgcgggcc aaacatgaaa aactgtttgc tatagcctgg
     6121 cgcgccagca atgttaaatt ctgctaagtc cagcgccgga agttttaaat cgagtagcgt
     6181 tgtctgaccg tctttacgtg ccagcaataa tgcggcgttt ttatcatttt ccagctgcac
     6241 atgcccctga gcgtcactgg ttgcctgagt cagagtctgc cctttctcat ttaataaaga
     6301 gacttcaatt ccttgctggg ccgcgccgtt ttccagactt tgggtaaaga tatccagacg
     6361 attgtgataa cggtgagctg aaacgccgat atcacttaac gtaaacagcg tcgcgggatt
     6421 actgtaatcg taacgtccag cctgattcat cacagccaga tacacgcccg cctgctgaag
     6481 cggtttgata tcgcccagcg gcagcaataa tttttcacgg gtgttacgcg caggattgag
     6541 atcaaaccgt ccggtgtaga ccagatccgc catctgcagc agtttgtctg actgccagtt
     6601 cgccagcgaa ttgcggtatt cccattggct aatgaatgct ggcagagatt ctggcttaac
     6661 gcggaagaag ttaacatcga cattattaac gttgagcgcc attaccggca gcccttcaac
     6721 gactttacca ggcagcagcg aaccacggct ggcaaaaccg acactgggtt ggatgtcgcg
     6781 ggtagttata gttttttcgt aatctttact gaaggttgcg ttgttgagtg ctttgacctc
     6841 cttgccaatt gtaacgatca aatcacgttt cggttcgagg tggcgtaaac gcagctcttt
     6901 aagattatct gatagctccc aggcaccatc cactttgccg ctttttttat cgacgacatg
     6961 aataacgcgt gagaaatcct gatccgggtc gagagggatg gagaacgtca gcaccagcgt
     7021 ggctgcacca tcaagttgga cttcagagag atccagcaat gtcagcgcct taccggcact
     7081 ctgttgggcc agtttttgtc gctctggcgc ggagagtttt gctgaactcg cgttttctga
     7141 agaggccgct ttagtaactt cagaaggcgc atcttttttc accgctgttg gcgcgttatc
     7201 gttgttgtcg caccctgcca gcgccagcat tagcatgcag gcggctacgc gtaacttttt
     7261 catttttcat ccctgccaca atggcccgtt agcaacgtcg aataattatt atgcgtgaga
     7321 atttacgtta tgtaattcag tatcacgccc atacaatccg aattttagtg agaattgtgg
     7381 ctcagacagc caaatcagcc tcttttgctt gtcgcttttc actaaacatc cgacaattta
     7441 gcctaacccc ggcaaaaatg gagatgccta tgtccacgac atggtttgta ggagccgact
     7501 ggctcgccga acatattgat gacccggaaa ttcagattat cgatgcccgc atggcgtcgc
     7561 ctggacagga agatcgtaac gttgctcagg agtatctgaa tggacatatt cccggcgcag
     7621 tgttttttga tatcgaagcg ctttctgatc acacttcccc gcttccgcac atgctgccgc
     7681 gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt taaccaggat aagcacctga
     7741 ttgtctatga cgaaggtaat cttttctcag ccccacgagc atggtggatg ctgcgcacct
     7801 ttggtgtaga gaaagtgtcg attctggggg gtggacttgc aggctggcag cgcgatgatc
     7861 tgttgttaga agaaggtgca gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc
     7921 ctgaagcagt ggtgaaagta accgatgtgt tattggcaag ccatgaaaat acggcgcaaa
     7981 ttattgatgc ccgcccggct gcacgtttta acgcagaagt tgatgaacct cgcccaggtt
     8041 tacgtcgcgg acatattccc ggtgcattga acgttccgtg gacggaactg gtgcgcgaag
     8101 gcgaactaaa aacgaccgat gaactggatg cgatattttt tggtcgcggc gtcagctatg
     8161 acaaaccaat tatcgtcagc tgcggctctg gtgtaacagc agccgtggtt ttgttggcac
     8221 tcgcgacgct ggatgtgcca aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg
     8281 ctcgggcaga tttaccggtt gagccattga aataagcttt ttacaggcaa taaaaaaccg
     8341 ccgaatttgg cggtttttta ttgctggtct ggttcgcggc ctttccagca ggttgacttg
     8401 tgttgccgga gcaacgcagg caccccacag tgttgaaata ctctctttta tgcctggctg
     8461 tgtcaagata ataaacagag taattattca aacatgagag tattattctt aatggccctt
     8521 ttcgccgtct cgcaaacggg cgctggcttt aggaaagaat gttccatgac cgtaaataca
     8581 ggcaccccac agtgcttgct atcgcggcaa tatcgccagt ggtgctgtcg tgatgcggtc
     8641 ttcgcatgga ccgcacaatg aagatacggt gcttctgtat cgtacttatt gtttctggtg
     8701 cgctgttaac cgaggtaaat aataaccgga gtctctccgg cgacaattta ctggtgatta
     8761 acaaccttca gagcagcagg taagcccgaa tgccgccctt tggggcggca tattttagat
     8821 tatccgattc tgtttaaagt cacgcaaaaa accaccccag cgacgttcat agaatggcgc
     8881 aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct gacaaatatc
     8941 gattggttca tcgccaggta atgtatcggt cgctacactt cccgccacct gaataatttc
     9001 ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct cttcgttctc
     9061 tttaattgaa caaataaaag cacgcttcac aggcttaatg gttttaaata aggtggtgag
     9121 tgaatcaatc atttgtgctg gcggctctgc gacttccgat aatatcagcg attcaccgcc
     9181 ttccaggatt tcctggctgc tcagcggatt tccctcttca ccaatcaaca aactgatttc
     9241 acgcggcata aattctttac cggtcggcag tttggcatta aggaagagcg tttcgccaag
     9301 ggtcatctca aacaatgtgc gaacgggcat tacgacaaat gcctgttcgt cttcaaccgc
     9361 ctgttgaagt gcttctaacg aggtaaaaaa aggaatgacg ctggtgccgt cttctttttc
     9421 ccagtgctgt aaatcaagcg cgctatcttc aaccacagcc tcgccctgcg ccgccgtacc
     9481 aggcacccag acggtggatt ccagtagagc acggaaaaag gccgggcggt gcgccggttc
     9541 ggttgctgct ttttccagca ggtcttcaag ttcatttttt gtttcggaca taagaaccac
     9601 aattcattca acgttcgggg cacaagtgcc tgatgcgcca tacttatcag gcctacaagg
     9661 gattcacaat ttgttgaatt tgcaatactt tgtaggccgg ataaggcgtt tacgccgcat
     9721 ccggcatatt agtttacgcc gttaacagat tagcaatcgt gcgcacacca agtcccgtag
     9781 cacccgcaga ccactgttca accggcgctt tacggtaagt cgccgagcag tcgatatgca
     9841 gccagccttg ctgatagttc tcaacaaagt gcgacaggaa gcccgctgcc gtgctcgcgc
     9901 ctgccggata cgccgcgctt ccggtattgt tcagttcggc aaagttagac ggcagctggc
     9961 tgcggtggaa ttccgccagc ggcagacgcc agaatggttc gttctcttgt gaggcactcg
    10021 ccagcaaacg accggcaagc gcatcgtcaa aactgaacag cgcgtgataa tcattaccca
    10081 gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg
    10141 cactggcatc aatcagacca tcggcaagca ccaggcgccc ttcggcatca gtgttcatca
    10201 cttcaacttt tttaccgttg cgataagtga tgatatcgcc cagcttgaac gcattgccgc
    10261 taatcaggtt atccgcacag cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa
    10321 tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt
    10381 ccataaacgc agtctgtttg atgctgtagc cgccggagtc aaaagtgata cctttaccta
    10441 ccaggcacgc gtacactggc gcttctttat cgccagttgg gttgtaatcc agcgccagca
    10501 ataccggaga acgttctgaa ccgcgtccga cggtgtgcag ccccatataa ccttgctcac
    10561 gcagatcttc gcctttggtg atccgataag tcacacgatc gctcgcgacg ttgctgatca
    10621 gatcaacagc acgctgtgcc agttgcgatg gtcccaattc ttctgccggt gcgttgatgg
    10681 tgtcacgcac ccagtcgatg atcatcaggc ggttatccag ttcctggcgc tgggcatcgt
    10741 ccagatccgg ccactccact ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg
    10801 cccagcatcg atccgcatcc cagccttcac cgcttaactg aacatgcttg atgcccagac
    10861 cgtcaatctt gcgcgccgca cgctggatca gccccagatc gtctgccccg ttcaaatgca
    10921 gggtaatgcc gtcattatta atgctgtaag ttgctttttc tccccagcgc gcgtcggcag
    10981 gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa
    11041 gggccgccag ttggcagccc caggattttt ccgctaaatg attcgcgttg caggaaggcg
    11101 gcaagtgagt gaagccccag gagcatagat aactatgtga ctggggtgaa cgagcgcagc
    11161 caacgcatct gcggcgtgaa gcatgacgcg gaaattattc ggcctcgtcc agccagacta
    11221 acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca
    11281 gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat
    11341 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt cccatactaa
    11401 cctctgttaa tgctcacgcg catggttgat cgtgtaacgc gggatttcga ccactaaatc
    11461 ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt
    11521 gtccagcatg tcgtcttcct gctctgagct ttctggaagc gagtcaaaac cttcacgaac
    11581 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcgatacc
    11641 gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc
    11701 agggcagaga tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
    11761 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
    11821 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca
    11881 cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg
    11941 cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt
    12001 gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag tctttgatca
    12061 tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa
    12121 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
    12181 tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc gcaaaacgcg
    12241 ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg
    12301 ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccactgga atagtggtat
    12361 tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga
    12421 tcacatcaag caacagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa
    12481 tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt ggacgaccga
    12541 aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
    12601 cttccagcac ttcatcagct tctacacccg cgtctttcag cgcgcgacga caagccaata
    12661 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
    12721 gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa
    12781 tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga atgcccgcct
    12841 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac
    12901 cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg
    12961 tcccgccacc gaggtcataa acggcgatca cgccttcctg accggaatcc agcccgtagg
    13021 cgatagccgc agcggtcggt tcgttaagta agcgcaggac gtgaaggccc gccagacgcg
    13081 ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta
    13141 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
    13201 ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt
    13261 tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
    13321 agcgtcccat caggcgttta acagaactaa ttgtgttggc ggtatcgagc gctgcattag
    13381 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca
    13441 ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca
    13501 gcgagttggt tgtgcccagg tcaataccgg ccgccagacg acgctgatgc ggcgcagcac
    13561 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
    13621 agcagttttt cttcgagttg ttcggcactg cttcgcagtt tatcgagaaa acgcagctta
    13681 cgcacggtat ccgccgccgc gtcccaggtc tcgttgtcta actgttcaac catcaactga
    13741 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
    13801 ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat caggaacgcg
    13861 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaagc cgtgcaaaga aagcaaatat
    13921 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct gattaatggt tgcagattgc
    13981 tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg atgatactga
    14041 cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata acgggcaggc
    14101 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
    14161 gcgaatcagg taaaacaacc cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
    14221 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg ttggtgaatt
    14281 taaacccttc gttcaggcct tcttttacga agtccagctg cgtaccgtcc agaaattgca
    14341 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
    14401 tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc
    14461 ccagacgtag gccaaaccct ttgccacggt tagccagaaa ggtatttact cgcgctgctg
    14521 cactgtcgct aagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
    14581 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
    14641 ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatagc ctgcgcttcg
    14701 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcagaaccg
    14761 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
    14821 aacttcatta cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg
    14881 ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
    14941 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
    15001 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
    15061 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
    15121 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
    15181 taggacggtt cgaggcttgc tgacgtacag gcggaaccgg aagaaaccgc gagatctttc
    15241 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
    15301 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
    15361 agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
    15421 cgataggctt cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg
    15481 cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt acgacgcaca
    15541 tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
    15601 actttcaact ggctcaggtc gataggcagt ttgcccacgc tctgggttgc atcaacgtga
    15661 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
    15721 atttcgttat ttacgtgcat gatagaaaca aggatggtgt cgtcacgcat cgctgcttcg
    15781 agttctttca ggtcgataat accgttacgc tgcggtgcga ggtaggtgac ttcgaaacct
    15841 tcgcgctcca gctgacggca ggtatccaat accgctttgt gttcggtttt gctggtgatt
    15901 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagac
    15961 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
    16021 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgggag
    16081 gccgggttac caaaggttcc gtccatcgtc ataaactgca tcattttctc ggcaacacgc
    16141 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
    16201 actccgtaca tcactcaatg caaggaacca ggctaccggc tggatgtacg accgtgttta
    16261 cggagcgcga tacgccccgg cctgattctg aatacttttt attaagcgcg taacttaacg
    16321 tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca
    16381 tccagcactt cctggttgtt aaccagttcg cctaaagtaa tgttattgag aaaaccggta
    16441 agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag
    16501 ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg
    16561 ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg
    16621 ctggaaacca gaccattttt acgcagacga gaaaacagtt gttccagata agaaagggaa
    16681 attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca
    16741 acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc
    16801 acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag
    16861 ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg
    16921 tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttatc cttccagacc
    16981 atgggcgggt tgagggcaat tattcgcatt agcgcaggcc tgttcccctc accctaaccc
    17041 tctccccgaa ggggcgaggg gaccgtccgt gcacttatct ggcttagccc tcaggcctta
    17101 ttacctttat tctgctgctc tatagaagcc agaatcccgc gcaggatatt caattcctgg
    17161 ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg
    17221 ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc
    17281 agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc attttcctga
    17341 gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat cgccaggttc
    17401 agcgagctgt attcagggtt agcggcaatc gcgacatgat aatgacactt ctgcaactct
    17461 tcattggtca ggccgacacg ttcgcgacca aacaccagcg ccaccggggt atttgccgct
    17521 tcagcaacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg cagcgtgcgg
    17581 gaacgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc gagcgtatcg
    17641 acgatgtgag cattaccaat cacatcgctg gcccccgccg ccagggcaat cgcctgggag
    17701 tcaggtttca ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca
    17761 cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt
    17821 tgcagcattg tctttcttcg tctaaagatt attcgcgcat cttatcataa aacgaagaca
    17881 gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag
    17941 agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaatttg
    18001 attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgac
    18061 ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct
    18121 tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat
    18181 gttcaatggg ttatcgatcc actggatggc actaccaact ttatcaaacg tctgccgcac
    18241 ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat
    18301 cctatgcgta acgaactgtt caccgccact cgcggtcagg gcgcacagct gaacggctac
    18361 cgtctgcgcg gcagcaccgc tcgcgatctc gacggtacta ttctggcgac cggcttcccg
    18421 ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaaact gttcaacgaa
    18481 tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt
    18541 cgtgttgacg gtttctttga aatcggtctg cgcccgtggg acttcgctgc aggcgagctg
    18601 cttgttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg
    18661 accggtaaca tcgttgctgg taacccgcgt gttgttaaag ccatgctggc gaacatgcgt
    18721 gacgagttaa gcgacgctct gaagcgttaa tgactcaggc gggtgatatc actcacccgc
    18781 cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc tttcgtcact
    18841 ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa ggttatggca
    18901 ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt tgccttctgc
    18961 gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac
    19021 ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg
    19081 caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc aaccatcatt
    19141 catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag ttggttaccc
    19201 gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg
    19261 ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc
    19321 agtgatgtaa acccacaacg gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt
    19381 cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cagtgatcct cgactccaca
    19441 tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta cttacttgat
    19501 gagagttaca gcggcgaaaa ttatatcgcc agcgtcagcc cgatcccgct tttactcatt
    19561 cacggtaaag ctgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca
    19621 aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat
    19681 cgtcacggcg acgtttatcg cgaacagatg gtgaacttta tccttagcgc gttgaatccg
    19741 cagaactaac ccatgatcgc tagcacgata atcattcaca aaatcacctt aagacatgct
    19801 aatccactgg tcaggacagt ttaagatgag aaaaattctg tgacgcttgc caacatttct
    19861 gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat
    19921 gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc
    19981 ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc
    20041 aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac
    20101 aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta
    20161 ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg
    20221 tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact
    20281 gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa
    20341 tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct
    20401 gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt ttaatccgct
    20461 ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca
    20521 ctggcagcgt cgggcgctcc agcctgtacc acctgatgag ccactgttta tggcactact
    20581 tttttccatg ttgcgggttc ccgatccatt gcgggatgcg catcagcggg acagacaatt
    20641 gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta
    20701 tgatgaacag gggttatgtg atcagcttta tacccacctc gcccaggcgt taaatcgcag
    20761 tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac tgtatccacg
    20821 cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct
    20881 ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa
    20941 cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc gagaagcgca
    21001 gattgagcaa cagttacgcg aactaacgtt actgccgctc aacattaaac atatgtcggt
    21061 aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg ctccttatac
    21121 catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacac tgacaacaca
    21181 tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact ttcgggcgca
    21241 aaaacattgc cggaagcgcc accagcgcca tcacccagaa cacgccgtgg cccagatatt
    21301 gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc gcgacggcag
    21361 agtaaaccgc ctgtaaacgg atgacttcgc taccctggcg agcagcaata tagcgcatgg
    21421 cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact atcaaccacg
    21481 gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc gctgagatca
    21541 acagcatatc gcgtgcacta caacggcgga aaagtttatt actcagtgca aagataatga
    21601 cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag tagccagctg
    21661 cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc aataaacaaa
    21721 cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgctgac caaccggtgc
    21781 tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga aagccgagca
    21841 gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca aacatagtga
    21901 ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac agtcgcactt
    21961 tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta
    22021 ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac gctacgtgcg
    22081 cccccgccca gaaggcgaca gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg
    22141 aaatcaggcg agaaggatcg ctgacgcggg gcgcgatgag caaactcccg agaaaacggg
    22201 caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca atccctttaa
    22261 gccagacgct ccagaaaggt agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga
    22321 gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct
    22381 ggagttaatc taatgttggt gcaagttgaa aaataagaat attcgagcct gcttcccgat
    22441 gtctgtaacg ccagttcaca agcactcaaa aactgcgatc cgtgacgcat gttgcaaaga
    22501 taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg atgaaatcgc
    22561 gtaatgccgg cttcatctcc cctttcttcc acgccatcag taaagcaatg gaaggcacat
    22621 ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata taaccgggta
    22681 tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata ttcgttgcca
    22741 cctggacgat atttggctgg ctgttttctt gtgcaaacca cgctttaacg atcggcgcaa
    22801 gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt tgcgcggcgg
    22861 tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact aatggttcgt
    22921 caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt aagccgacgt
    22981 cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc accagctcaa
    23041 tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcagt aaattcactt
    23101 ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc
    23161 gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga aaacattctc
    23221 ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca ccgacacagt
    23281 tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg
    23341 cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca
    23401 tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact atttcacata
    23461 ttagcaacca accagcaaca tccttatgac acaaaaatag aaggtcaata catcttatct
    23521 ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac tgattgacac
    23581 ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc aactggagct
    23641 tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga tcccaaaacc
    23701 cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc gccagaaaga
    23761 cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc
    23821 agattgcggc aacactcgcg cctttacctg cccgtatcac ggctggtctt atggcattaa
    23881 cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc
    23941 ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaagggctga tttttggcaa
    24001 ctgggatacc agcgcaccgg gcctgcgtga ttacctgggt gacattgcct ggtatctgga
    24061 tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt
    24121 gatcaactgc aactggaaat tcccggcaga gcagttcgcc agtgaccagt atcatgctct
    24181 gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca gcgataagcg
    24241 cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg ccctgcaatt
    24301 tggtcaggat ggtcacggca gcggcttctt ctttactgaa aaaccagatg ctaatgtctg
    24361 ggtcgatggc gcagtttcca gctattaccg cgaaacctat gccgaagctg aacaacgttt
    24421 aggtgaagtt cgcgccctgc gcctggcggg tcataacaat attttcccga cactttcctg
    24481 gctcaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt
    24541 gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa
    24601 cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact cggagaactg
    24661 gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga
    24721 aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta ctaactatat
    24781 cttttcagaa actgctgctc gtggaatgta ccaacgctgg gccgatcttc tgagtagcga
    24841 aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga tgaaatgagt
    24901 gcgcaagttt cactagagtt acatcaccgc attagccagt ttctctttca cgaagccagc
    24961 ttactggacg actggaaatt tcgtgactgg ctggcacagc tcgacgaaga gatttgttac
    25021 accatgcgca ccacggtgaa cgcgcaaacg cgcgaccgcc gcaaaggtgt tcagccgccg
    25081 acaacctgga tttttaatga caccaaagac cagctggagc ggcgaatcgc ccgtctggaa
    25141 acgggcatgg catgggcaga agagccgccg tcacgcaccc gccacttaat cagcaactgc
    25201 cagataagcg aaaccgacat cccaaacgta tttgctgttc gggtgaatta tctgctttat
    25261 cgggcccaaa aagagcgcga tgaaacgttc tatgtcggga ctcgttttga caaagttcgc
    25321 cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga tcaagcggta
    25381 atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg
    25441 cggatgtgcc ggagggtgag gctctccgga tcgatacctc gcccgtcatc gccttgttca
    25501 acgttggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat gcgtcgatgt
    25561 cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc gccagttttt
    25621 gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc acttatccag
    25681 tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca tgagcgatct
    25741 gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg cgctggtcga
    25801 gcgatttatc gaagaaggcg cgcaggttgc cacgctggaa ctgtcggcgg cgaaagtcgc
    25861 cagtctgcgt cagcgatttg gtgaacatat tctggcggtg gaaggcaacg tgacctgtta
    25921 tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc tggattgttt
    25981 tatcggcaat gcgggcatct gggatcacaa tgcctcactg gttaatactc ccgcagagac
    26041 gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc tgctgggtgc
    26101 aaaagcctgc gctccggcgt taatcgccgg tgaaggcagc atgattttca cactatcaaa
    26161 tgccgcctgg tatcccggcg gcggtggccc gctgtacacc gccagtaaac atgccgcaac
    26221 cgggcttatt cgccaactgg cttatgaact ggctccgaaa gtacgggtga atggcgtcgg
    26281 cccttgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc
    26341 aataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc aatttttccc
    26401 gcaaccggct gattttacgg gtccgtatgt gatgttggca tcgcggcgca ataatcgcgc
    26461 attaagcggt gtgatgatca acgctgatgc gggtttagca attcgcggca ttcgccacgt
    26521 agcggctggg ttggatcttt aaggaagtac gatgaaagaa aaaacgatca ttattgtcgg
    26581 gggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt tcaccggtga
    26641 gctgcatctg ttttccgatg agcaacatct tccttatgaa cgccctccgc tctcgaaatc
    26701 catgttgctg gaagattccc cacagttgca gtctgtgtta cccgctcact ggtggcagga
    26761 aaacaatgtt catctgcatt ccggtgtaac catcaaaaca ttgggccgcg acacacgaga
    26821 gttagtgtta gccaacggcg aaagctggca ctgggatcag ctttttatag caaccggcgc
    26881 ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta ccctgcgcca
    26941 tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt
    27001 cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca gatgtaaggt
    27061 gacagtgatt gaactggcgg caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg
    27121 ctatctttta cagcgccatc agcaggctgg tgtgcgcatt ctgctcaata atgccattga
    27181 acatgtggtc gatggtgaaa aagtagaact gacgctgcaa agtggcgaga cgcttcaggc
    27241 cgatgtggtg atttacggta ttggtatcag cgccaacgac caactggctc gcgaggccaa
    27301 ccttgatact accaatggca ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat
    27361 ctttgccggt ggcgatgtgg caatcactcg tcttgataat ggtgcactac accgctgcga
    27421 aagctgggaa aacgccaata accatgcgca aattgccgct gccgcaatgt tagggctacc
    27481 gcttccgcta ctgccgccgc cgtggttctg gagtgatcag tacagtgata acttacagtt
    27541 tattggcgat atgcgtggcg atgactggct ttgtcgtggc aacccggaaa cccagaaagc
    27601 gatttggttt aatctgcaaa acggcgtgct tatcggtgcg gtaacgctga atcagggacg
    27661 tgagattcgc tcaatccgca aatggatcca gagcggcaaa acgtttgatg cgaaacagct
    27721 gacagatgag aacatcgcgc ttaaatcact gtaaccagga taattagcga atatctcaac
    27781 gcctggggcg tggcgaggta ccagagtgtg tattacgttt aaatcacatt atcttgcaaa
    27841 gggattggtt atgaactcac tacgttattt tgattttggt gctgcccgcc ccgttttgtt
    27901 attaattgcc cgtatcgccg tggtcctaat tttcattatt tttggttttc ccaaaatgat
    27961 gggctttgac ggtacggtcc aatatatggc ctcgctgggc gcaccgatgc cgatgctggc
    28021 agcgattatt gcggtagtta tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt
    28081 cacccgtccg ctggcggtac tgtttatttt ctacacgctg ggtacggcgg tgattggtca
    28141 ccattactgg gatatgaccg gcgatgccgt cgggccaaat atgattaatt tctggaagaa
    28201 tgtcagtatc gctggcgcgt tcttgctatt ggcaattacc gggccggggg caatttctct
    28261 cgatcgccgt taggaaagat gccggatgcg gcgtgaacgc cttatccggc attttataaa
    28321 ttacagccac tctacccgca acgacatctc tgaggttgtt gattcccccg gcgcaagcgc
    28381 aatgaggtca cccccttccg ggcgatgatg gtcatccggc gcatggctca tcggctccag
    28441 acagaagaaa tcaaacgcat atcctttatc aaacgccggg tcggaaacaa agatgaaata
    28501 acacggtgca ggtggcgtcg tttctatgat gatggcataa ccctcctgcg gctgctcgat
    28561 gcgggcctga ccattccatc cggcaaaacc attgttcacc cactggcgcg gcaacggcgc
    28621 tagctggtta aaatccagtt cttgcggtag ctgctcgcaa aactcacccg ccagccactg
    28681 ctcccgctcc agccagtaac cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg
    28741 aaaataagga tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacaga
    28801 gagcgtcacc gtcagcgtat ccgccgttaa atgaaacgcc tgacttaccc gatagtgata
    28861 gacaccgctg cgatgttcat acaccagaca caaactatca tcgctgtgcg agacacattg
    28921 ccactcgccc agccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg
    28981 cagttgatac tcacgcccct gccagacaaa acggttgccg ctcacccggt ttgcaaatgg
    29041 caccagcgga aaacaagagg catcggttgc cacaccgctt tttttaccag gacgtaagag
    29101 cggcgtcgta tcgcgccaga agccttcgat tacgccgccc tggtcagaaa cgcccagctt
    29161 tagcgacccg tgggataagg tatagatggt catccgctaa ctccttaatc cgggaagtta
    29221 atcacaactt tcccgcattt gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc
    29281 agcgagaagc gatgggtaat ggcgttacgc ggccacagct tccagtcagt caaatcgtgg
    29341 gcgcattttt ccatatggaa cagactggtc acccaggagc caataatccg ccgttgatgg
    29401 tgcatcagat cggcgctgac ctcgaattcc acttttccgg tttcaccaat gtaaaccacc
    29461 cgcccccagt cagcggtgga ttgcagtgcc agtaagcgac ctgcggcatt accggaacaa
    29521 tcaagcgcaa catccgcgcc gccgtgagtg agttcggcga ttatctgcgg cagaccttca
    29581 gtggtcgcta aatagccgtg atccatcact cctaactgtt ttgccatcgc cagacgttcc
    29641 ggcagcatat caacgccgat aatccgtttt gcaccgcgac ctttcgccag catcatcccc
    29701 atcatgccga ctggccccag accgaccacc agcacgttat cactgccgga aacttcgccg
    29761 cgcaaaatcc cttcataggc cgtgccaacg ccgcaactga taaacgcacc atcttcgtag
    29821 ctcagcgcat ccggcaggag gatcagatct ttttcttccg ccagcaagta ttcggcatga
    29881 ccgccgtcac gctgccagcc gtaagccgct tttccttcgc cagtacaaga aataggaaaa
    29941 ccgcgacggc agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcggtcg
    30001 ccctctttaa aatggcggca gccttgcccc atcgccacaa tctgcccgca cggttcatga
    30061 ccgttgataa atccctagta caacggttta tcggatgccg ccgccgtagc gcggtgctgg
    30121 tggtagatat agtggacatc gcttccgcaa attccggagg atttcatttt gatcagtacc
    30181 tggttaatcc ccggcgtcgg cactgcaact tcccgcagat cgacggtcga atttcctggt
    30241 aaataagctg ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc
    30301 cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt cgctttactg ctacgctggg
    30361 tcagcaagat attcgccagc accgccacca cgatgatgac gccgcgcacc acctgctgga
    30421 aaaaggagtt aatcccgagc agcaccagac cgttaccgat tagcgtaatc accagtacgc
    30481 caagcagcgt accgaacaac gagccgcgac caccggaaag cgccgtaccg ccgaccacga
    30541 ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc
    30601 gcgccgccaa caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aagataagaa
    30661 tgcgtacccg acgaacgtta ataccgcaca actgcgccgc cgtggcatta ccgccaacgg
    30721 caaaaaccga gcgcccgaag gcggttttgc ggctaatgaa cacaaacagc gcaaacagca
    30781 ccatcatgat cagcgcggat accggcacac cgagaaattg tccgcccagc cagtccagca
    30841 cctcgttttc gtcaatcggc actggcagag cgttggtcat aaacaagccc attccgcgca
    30901 gggcgctcca cagccccagc gtggccacga aacttggcac gttaaacacg ccacgcagca
    30961 ccccggcgag cgttcccatc agcgcgccta acagcaacac cagcagacaa gcaatcgcca
    31021 acggaacttc aaattgcagc aaaaatgcca ggcataccga gacaaaagcc accatcggcc
    31081 caacgctgac atcaatttca ccggagataa taatcagcgt catcgcccag gcggcaatcc
    31141 caatggtggc ggcatcgcgc agcacgttca tctggttatt caatgagata aagccaggcg
    31201 cgttcaggga gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa
    31261 tatggtgact gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg
    31321 aagcagacat cttcaggttc ctcggtaaaa ttcatcagtg cacagacaga atggcggaca
    31381 tcagctcatc cacattgacc ggagcgtgaa actcctgcga gaacgtgccg tgctgtaata
    31441 acaggatgcg gtcacacacc aacggcaact cctccacttc actggagata aacaccacgc
    31501 tttttccttc ggcagccagc tcacggacaa tacggtaaat ctgctgtttg gcttcgatat
    31561 cgacgccgcg cgttggctcg tcgagcaaca aaatctggct ggcggcatag acccaacgac
    31621 cgatcactac tttttgctga ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg
    31681 cggccttgac cgtcatccgc tgcatcactt cttcggtcag gcggcggatg gtggaccatt
    31741 gcagcactcc gttggcgctg attttttgcc gattggtcag cactgtattt tcgtcaacgc
    31801 ccaaccaggg aatgatcccc gcttctttgc ggttttctgg cgtatagcca atgccgcgtt
    31861 tcagcatgtc gccgtaatcg gggcgcgtga ttttctcgcc gttgataaca atttcgccct
    31921 gttcatacgt ctccagccca acaatcgcct tcagcaattc actgcgccct gcccccagca
    31981 gaccagcaat gccgagcact tcgccacgac gtagcgtaaa gctgatatcc tccagcttgg
    32041 gcttatggcg taacgcacgg acttccagca cggcctgatc cacaatttcc tgaggtgcaa
    32101 ccggcgcaat atcaacgtga tcgcgcccta gcattagcga cacaatatga tgcgtggaag
    32161 tgttttcgag catcacatcg ccagccacct gcccgtcgcg cataacggtg gcactggagg
    32221 caatgcggcg aatttcttcc atccggtggc tgacataaat caccgccacg cccagtgctg
    32281 acatcttttt caccgctctg atcaccagtt caacttccgc actcgcaagc gaactggtag
    32341 gttcatcaag aatgaccacg cgcggctcgc ccttcatcac ccgcgcaatt tccaccagct
    32401 gcttatgcgc cgggcttagt gttgaaacaa gttgttcagg actaacgtca acgcccagcg
    32461 cctgtaagca acgttgggca tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc
    32521 ggggccactg accgaggcag aggttttccg ccaccgtcag cccttccacc agacttaact
    32581 cctgataaac cgcgcgtacc cccagttcag cggcacggcg agtcagcgta gcttcgtcac
    32641 cttccagtcg cgtctcgcca atccagattt caccgctatc cggacgttca ctgccggtaa
    32701 gcattcgaat gagggtcgat ttgcccgcgc cgtttttgcc taacagcgca cgaacctcgc
    32761 ctttattgag cgtgaagtta acgttatcca acgcaacgac gccgggataa cgcttatttc
    32821 ctgccaccac ttttgctacc gggactgcct ctgttgccgt gaacataggg acctctgcga
    32881 atcagcgatt agggcagacc atcaacgtgc gttgccagcc actgtttacc gtcttccgtt
    32941 ttggtataga gatcgatagg cacctgaatc actttttcac cgtcggcttg tttattgata
    33001 accttcaatg tttgcgcgaa aacagcattg cccattttct taccggaaat atccactacc
    33061 gctttcagca cctgattgtt ttccagctcc tgagcaattt cggttgtcat atccgaaccg
    33121 aaaacagcaa tttttccggc ctgattttga ttacgtaccg cttttaccgc gccgagtgtc
    33181 gcaccgcccg attcccccat aatggcgttg agatccggcg tggagataat cagtttttca
    33241 ccaacggaaa tcgctttatc taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg
    33301 ggaacgcggg attttaatac ttcttcaaat cctttacgtc gctgcacaca aacttcaaag
    33361 gcttcgcaat tgatgacggc aattttcggc tggtcaattt tattggcaat aaaataatcg
    33421 gcggcagcgt tacccagttt tttaccaaat tccagcgggt cacccaccag atacgccgag
    33481 acatatttat cgaccccctt ttgattaata caggtgttgt agcaaatcac cggaataccc
    33541 gcttcactgg cgcgacgaac ggtacggcta ctgccatttt cagacactgc cgataaaata
    33601 atagcatcga cattacgcgc cacgagggta tcaacaaagg tactttcttt cgaaatatca
    33661 ccctgggcgt tagtttcaat taactgtacc tgtactgaag aatcttttgc cgcatcctga
    33721 actccctgac gcactccagc gtaatatccc tgagtatcaa ggtatattgc gccaatggtc
    33781 atttctttat ccgcagctct ggcaaatagt gcgctaccta atagcgtagc cattaataat
    33841 agattacggg ttgttctcat ttttttaggc atagagcctc ctgtagggtt tttattaaca
    33901 acggcttatt ctaattattt tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt
    33961 aatagggttt agtgaacgta gttaaaaata aaaggcatca cggtggtgaa taatgccgct
    34021 ttcggccagg ccggattaat cgccgtcaat tcagcgcgag cagattcgta ttgcgcaatc
    34081 tcgcctaatc ccgcggccgc cagcatcgcc accatcaggc atttttcttt atgctgctgt
    34141 tgtaaatcgc catacagcga caacaagtca ggctgcgaga cggcaaagaa atccgcttcg
    34201 atactggttt tcgccatctc ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg
    34261 gtgtgttgtt cgcccagtaa tcgcagcgcc attccttgcc agaagaggta atcaaccggc
    34321 tgatcgttgt aatagctgtg gatgttaatg gtgcgatcgc cggtcgccgc cagatgtaaa
    34381 cagcgcgtcg cttcagtttc atcgccctgg gctttggcgc atatcgcctg ccagaaccag
    34441 atgtcgttat cagtttgccc cggtaaacgg ccttcgctta aattctccgg ataatgcagc
    34501 gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg catcaagatg ctgccaggcg
    34561 cgtaataact ggttgaggat aaactgactg gtgaccttcc cttccccgcc ttcccacggg
    34621 tggaatttgc gcgtggcgag aatgtccgcc gctttgtctg cctgccccgt gagatgccac
    34681 aaattgagca gttctgcggt catgtcgtcg cgtttcagcg caatttccag attattttcc
    34741 agacgcgcca gtcgtttctc cggtgtggtt ccacttagct tatcaagcaa atcccgttcg
    34801 aaaagcagac gtgcatcctg cggcgcaagc tgataagcat tatcaagata acgcgcggcc
    34861 agctcataat cgtgttgctt attccacgca tggatcgcta acccgcgcca gccgtcggca
    34921 aactccggcg acatctctac gcaacgttgc cataaggcaa tggcttcgtt gtagctacgt
    34981 ttgttgtagt agaagcaggc cagtaaatgg cgagcaaacc agcactcttc aatactctcc
    35041 agcgccgcca cttcttccag cgtattcggg aaacggacaa actgcgggaa gacatcaatg
    35101 gcttttgcga ccagttcgcc acgttcggct ttcggcagca agctggcttg caggtaaagc
    35161 ggcagcgtgc gctggcagtc cagtgcattc agcatctctg ccgcgagggt gggcattccc
    35221 cagttaatca gttgcccggc ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg
    35281 cgccactgcg cgagagctga ttcgctacga ccatcgaacc agttcaggca ccacagagtg
    35341 gcgttcagcg gataatcgcg cagcagtttc tcgcgctgca aacgcgcgtt gtcctgacga
    35401 ccacttaaca ccagcagcag attatgcagg caaagcactt cctgattgat tgggcaggcg
    35461 cgaagacttt gttggcaaaa atccagaccc gcgtcgaagt taccattacg cgccgccagt
    35521 cgtgccagac cataatagcc tccggcttta ctgttgccgc tccagaccgc acgccagaaa
    35581 tcctcttcgg cttgttgata ttgtccctga cgttcgtaag cactggcgcg aatcaaactc
    35641 gcctgtccgc actgcggatt tttgttcagc gcatgtgcgc gtttcagagc ctgagtggca
    35701 tacgccaccg cttgcgggaa atctgcgcgg ttatactcca gcatcgccag cgccagatta
    35761 cagcggtaat ccagcggatc cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg
    35821 ctggcgtgat gatattgctc cagatgctga ccgataaacc aggcttcatc tgtactggta
    35881 atgtcttgtg ctgacagtgg cgctttggcg atgtccggca acggcaactc ttgcggctga
    35941 tgttcctgat aactcagtac aatattgccg tcggcatcag agagctcaat ggtcagtctt
    36001 tcaggattga taccgtgtaa cacgccctgg atggcggtcg ctggtgtcag ggccacggca
    36061 tcatcgagca acgcgtcgca tttgccgatt tcgcggatcg ccaggcggta tccgttcaac
    36121 ggagagatgg catacagccc ccactcaatc ccccgctcac tacgctggag tttgattacc
    36181 gcatcgcggg aggcgttttg caccatgccc agagaatggt aaggcaggaa aaactgctca
    36241 aaacgcttct cttcgtaagc atcaagccgg gtaaaatcag gctggttatc ggcaaaaata
    36301 ccggtcatca gttcgatata cgggccgtta ttatcagtta ggctcttatc ccacgcctgg
    36361 ccaaattcac tgtgtcccca gctccactgc tttttacctg gcgcaatatg gtggttggca
    36421 acgtgtagca aaccgccatc ttcatcgtga caccacgcgc caacaaaatc gtactgtgat
    36481 ttttcagcca tatatgaggt tggaacgggc acatttttat agcgagaaat gtccactcca
    36541 gcggagtagt ccactttgta gtaagtgccg gtggcgatag ggaaagcgga gacggcccgt
    36601 ttgccgtgat caaacaccgc cgttacatcc ggcggaaaga cgctctgatg cccttcaccc
    36661 cctttcactg ccgggttggc ccaccacaag aaatgacgcg gcgtggcgtt gccgttatag
    36721 acgcggctgg cgatttccag cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc
    36781 tgtaaaccat gcatcggctc cgtttcgcct acccacaccg tctgtgcgcc gtcttcatgg
    36841 gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc gatggtgttg cggccagtta
    36901 aactcaatcc cgccggagat ccacggtccc agcagcccca ccagcgcagg tttaatgact
    36961 tcattgtgat agacaaaatc gcgctgtttc actttatccc atgcgcgatg cacccgaccg
    37021 cccaattccg gcaggatcat cactttgatg tagtcgtttt ccagccacac cgcctgccag
    37081 gatttcaggg ttttctgctc gctcagcgta tcggtcacgc cgtagggata aaccgcgccg
    37141 gacgatccct gataaacgcg attttccagg aacatgggat gtatatcctg cggcccggtt
    37201 tcataggtcg ggatctcaac gcgctcttgc cacactttta ctggagtcat ggtgccctca
    37261 atattaacaa gacatactga attaaaagat ttgtggcagt gtattgaaca atctggcaat
    37321 gttttcgcgg aataatcacg caattaacta aacaaggttt agtgaagatg agagcctgca
    37381 ttaacaatca acagattcgc caccataaca aatgcgtgat tctggaactg ctgtaccggc
    37441 aaaagcgcgc caataaatca acgctggccc ggctggcgca aatttcgatt ccggcagtca
    37501 gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt gaatatcgac gatgaaagcc
    37561 agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt
    37621 gtctgaacgt gacgcccacc agtattgagt gtcaggttgc taatgcttgt ttaagtccga
    37681 aaggtgaatt tgagtattta cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa
    37741 tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc aatctggcgc
    37801 tggcaatcca cggtcaggtt gatccagtga ctggagtatc gcaaaccatg ccgcaagcgc
    37861 cgtggacaac gccggttgag gtgaagtatc tgctggaaga gaagctgggc attcgggtga
    37921 tggtcgataa tgactgcgtg atgctggcgc tggcggaaaa atggcaaaat aattcgcagg
    37981 aacgggattt ctgcgtgatc aacgttgatt acggcattgg ctcgtcgttc gtgattaacg
    38041 agcaaattta tcgcggcagt ttgtatggta gcggacagat tggtcacacc atcgttaatc
    38101 cggatggcgt cgtctgcgac tgtggacgtt acggctgcct ggaaaccgtc gcctcgttaa
    38161 gtgcattaaa aaaacaggcg cgggtatggc taaaatcaca accggttaat actcagcttg
    38221 atcctgaaaa actgactaca gcgcagttaa tcgctgcctg gcaaagtgga gaaccgtgga
    38281 tcaccagctg ggttgatcgc tctgccaatg ccattggttt gagtctgtat aacttcctca
    38341 acatcctcaa tattaatcag atttggttgt acggtcgcag ttgtgccttt ggtgagcact
    38401 ggcttaatac tattattcgc cagacaggat ttaacccgtt cgaccgcgac gaaggaccga
    38461 gcgtgaaagc gacgcaaatt ggcttcgggc aattaagccg cgcacaacag gtgctgggaa
    38521 ttggctattt gtatgttgag acgcagttac gacagatttg atggcgcgat aacgtagaaa
    38581 ggcttcccga aggaagcctt tttcaacaga ttaaccgttt cgcttatgcg taaaccgggt
    38641 aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga
    38701 tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctact tctttgaagc
    38761 cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac gggctcttcg
    38821 gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg
    38881 cttctttacc ggtcaggttt ttatcgacca gatcaaccag gaacagatgg ttatcagtac
    38941 cgccggaaac cactttgtag ccgcgttcca ggaacacttc taccatcgct ttagcgtttt
    39001 tagcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg
    39061 ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt
    39121 tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcagaccg ccgcgcggac
    39181 ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt
    39241 agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg taagcgccga
    39301 tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat gcagagaagc
    39361 caccgataat cattttcggt ttgtgttctt tggcttgttt ttccagatcg gcgtagtcga
    39421 tatgaccggt agcatcaata ccgtaaggaa caatgttgta cagtttaccg gagaagttaa
    39481 ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac
    39541 ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga
    39601 cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga
    39661 tatcaacata ctcgcaacca ccgtagtagc gtttgcccgg ataaccttca gcatatttgt
    39721 tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga
    39781 tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg
    39841 catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac
    39901 aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa
    39961 tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac
    40021 gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga
    40081 tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc
    40141 agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaaggaaga
    40201 ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga
    40261 aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact
    40321 caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa
    40381 cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga
    40441 aaaaatcgcg cagaagcaca ccagtttcca gatcaaaccg gaacagtaca acatcgtcgg
    40501 tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc
    40561 gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta
    40621 taacgaaaac gccagcaaag ccggtggctg ggaaggtact cgcgatttcc gcattgtggc
    40681 taaaacacct cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc
    40741 agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc
    40801 gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat
    40861 tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt
    40921 tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg tcgcagatga
    40981 cacaccagtg acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg caatgctcga
    41041 cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg
    41101 cgatgttcac gcctttgccg atgaagttaa ggaactgggg cagtcactgc cgcgctttac
    41161 cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag
    41221 cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt
    41281 ctatctctgc ggcccggttg gcttcatgca gtttgccgcg aaacagttag tggatctggg
    41341 cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtgatttga
    41401 tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc
    41461 ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt taccgtcacc
    41521 gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc
    41581 cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg
    41641 gtacagctcg gtatggcctt tctggcggcc aaaacctttc acctcagtga ccgtcatgcc
    41701 ggtaatgccg acttcagcca gtgcttcgcg gacatcgtcc agcttgaagg gtttaataat
    41761 cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga tctgctaaac
    41821 gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag
    41881 cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc
    41941 tttggtaatt tgcagcagct tacgcaaata gttgagttca aactgattac gtgcctcaac
    42001 aaaggttggc agcgccgtat tttcaccctc cagcgcctgc tctactaatg catcgctaat
    42061 caccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt
    42121 acccggccag ctcgcggtca tcaggcgttt catcgcatcg gtggaaaacg cgcggacaaa
    42181 cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc
    42241 tgtgcgctcc gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc
    42301 ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg cagaaataat
    42361 ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa
    42421 cacgcgcaac agtttgacct gcaacggcgc aggcatatcg ccaatctcat cgagaaatag
    42481 cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc
    42541 gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcaggcaatg caccacagtt
    42601 aatagcaata aatggtttgc tattgcgcgg gctggcgttg tggatagcct gggcgaaaat
    42661 ctctttcccg gtgccgctct ggccattaat caaaacgctg acgtctgatt gcgccaccag
    42721 ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaatcg cctcgcgcca
    42781 gcgttcgtcg gtggctggcg cggattgttc cagcgcatca tcaattgcct gatatagcgc
    42841 gtctttatca acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc
    42901 atcgggaata gaaccatgcg cggtaagaat aattaccggc attcccggct gcactttctg
    42961 gatttcagca aacagctgca taccgtccat ttcatccatc cgcaggtcgc tgatgactaa
    43021 atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac
    43081 gctgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc
    43141 gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg
    43201 cgggcttttc actctcgtgt ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg
    43261 agagctggcg ttcaatatca gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct
    43321 gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga
    43381 gtttactgta gcgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc
    43441 agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg
    43501 cgaccagctg gcggcgctca tatggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt
    43561 taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag
    43621 gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg
    43681 atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat
    43741 aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc
    43801 aacctagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt
    43861 gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca
    43921 gacagtttgg acatggacag cgcggagaaa ccggagcgta catgtcgtac gtgaggattt
    43981 cgagcactgc ccggggccga actgacaaat aaaatagcct gatgggatag gctgttagca
    44041 ttatttcgtg tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc
    44101 gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc
    44161 cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa
    44221 gatcatggcg cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac
    44281 ccgcgcacca tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac
    44341 cgcattggag taaagattat ccagtacgct catcagcagc attggctccg ccaggcaagc
    44401 tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc
    44461 agaaactact gtctccacca gcggtgctaa ctcaacatta tctagttcca ccgcactgtc
    44521 cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcggctgct
    44581 gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc
    44641 agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa
    44701 atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag
    44761 aatacgttgc ccaaccgagc gtaactcgct cggtccactg aacgaaacgc tattgcccag
    44821 agaacgccct tcccccaacc gattgatcat ccgttcaata tttttcaccg gcccgataat
    44881 catccgcgtg aaaagcagta ccattaccag actcaccaga aatagcacca gcgattgcca
    44941 accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac
    45001 tactgtgcgc gtggcctgta ccatttcggt attggcactg gcaaaggctt ccagacgcgc
    45061 ggcagcagca gcatcgggac cactgttgtt gcactgaagt tgggccagat tgttcaagtc
    45121 ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat
    45181 ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt catccagcac
    45241 gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcattg cttcgctgcg
    45301 ccgggcatcg ataagcgtgg tgcggttgac cagcgccgcc tggtcgctaa gcgcattcag
    45361 gctttgccag gcttgccatg ccagcaccaa caggggcagc agaatcagca aaaatgccag
    45421 cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc
    45481 gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa
    45541 ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga
    45601 tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt
    45661 ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact gattactatt
    45721 atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt
    45781 gaggggttta tggcttcccc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga
    45841 gacaacttaa gaaaaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa
    45901 agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct cccgaagttg
    45961 ggagcttata atagcggttg tatttaatgc cggatgcggc gtgaatgcct catccgggct
    46021 gcaaaaccaa tgagtttgac ggcttacccc aactgcttac gtgcattgcg gaaaatgcgc
    46081 atccatgggc catcctcgcc ccagttttca ggatgccagg agttgctgac agtacggaaa
    46141 acacgttccg ggtgcggcat cataatggtg actcgaccgc tttcagtcgt gaccgccgta
    46201 ataccgttcg gagaaccgtt cgggttagcc gggtaggttt cagtgacttt gccgaagtta
    46261 tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg cgccgcatca
    46321 cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga gcccaccatc
    46381 ccctgcaaca gcagcgacgg gctttgggtc acttcaacca agctgaaacg cgcttcaaag
    46441 cgatcggagg tattgcgcac aaaacgtggc cacaactcac tacctgggat cagttcacgc
    46501 agattagaca tcatctggca accgttacat acccccagcg ccagcgtttg cggacggtgg
    46561 aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt cgcccaacct
    46621 tcaccggcac ccagcacatc accgtaggag aaaccaccgc acgcgaccag ggcgtggaaa
    46681 tcctccaggc ccgtgcgtcc agccagcagg tcgctcatat gcacgtcgat agcatcgaag
    46741 cctgcacggt ggaaagctgc cgccatttca acatgcgagt tcacgccctg ctcacgcagc
    46801 acagcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc ttcgttgata
    46861 tcaaacgaca gtttcacatt caggcccgga tcggcgtcgt ttgatttcgc ctgatgctcc
    46921 tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt ttctgcccac
    46981 cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc ggtaatcaca
    47041 aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatcagcaag cccatgctgt
    47101 gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatgac cgcacccagt
    47161 tcttcgttaa acaacgccgc caggcgatcg tcacccagag tggcgatatc cgcgtcaatg
    47221 ccgcaatgac cagcaaaggc catttccgcc agcgttacca gcagaccgcc atcagagcgg
    47281 tcgtgatacg ccagcaactt acgctgtgca accagcgcct gaatcgcgtc atagaagcct
    47341 ttcagttgcg cgacatcgcg tacatctgcc ggtttgtcgc caagctgacg ataaacctgc
    47401 gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag cagcagtgcg
    47461 ttatcttcgg tagaaagctg cggcgtgatg gtgtgacgta catcttccac gcgggcaaat
    47521 gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc ttcctgccag
    47581 cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag cgccggacaa
    47641 agctcttcgc ccacggcttt aacggcttca tacaggcccg catcttcacc agggtggcct
    47701 gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat ctgtgttgcg
    47761 gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa atccagcagc
    47821 gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc gaggctggcg
    47881 gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg atcgcgcgcc
    47941 accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc cgccacggtc
    48001 ggcaaatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg gaccagcgca
    48061 tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt accaagcagg
    48121 acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag agaaagatgc
    48181 agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt acacagttcg
    48241 tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg ggattcgtta
    48301 caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg cagttcaaat
    48361 ttaccgccgc gcccgccgtc gctcaccagt tccggcatcg cgttagaaag accgccagcg
    48421 ccgacgtcgt ggataaacag gattgggttg gcatcaccaa gctgccagca acggtcgatc
    48481 acttcctggc agcgacgctc catctccggg ttgtcgcgct gtacggaagc aaagtcgagg
    48541 tcggcatcag actgacctga cgccatggaa gacgccgctc cgccgccaag accgatgttc
    48601 attgccgggc cgccgagaac gaccagtttc gcaccgacgt tgatctcgcc tttttgtacg
    48661 tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg ataaccgcgc
    48721 agctcttcgc cgttgtggct gttcactttt tcttcataag tacggaagta gccattcagt
    48781 gccggacgac caaattcgtt gttaaacgcc gcgccgccca gcgggccttc ggtcatgatg
    48841 tccagcgcgg tgacaatgcg ctcaggctta ccgaaatctt cttcccacgg ctgttcgaag
    48901 ccaggaattc gcaggttgga aacggagaaa ccaaccaaac ccgctttcgg cttcgcgccg
    48961 cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc gcccggccac
    49021 ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcagaatatg cgccggttcc
    49081 tgatggaaat cgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac ttcagaacct
    49141 tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt ggtttcgaaa
    49201 gtatttttga tcatcttgaa cagcgatttc ggctgctgtt caccgtcgat aatccagtcg
    49261 gcattaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat atacagctcg
    49321 atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc aatttcatct
    49381 tccgccagag ccaggccaag acgcaggtta gcgtcgatca gcgcctgacg gccctgcccc
    49441 agcaaatcaa cgctggtaac cggagtcggt tgatggtggg caaacaactg ctctgcatca
    49501 tctaaagcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt aacctgctgc
    49561 cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg ctcaaggcgg
    49621 tttacctgtt gtagcccgca gttatgagca atatcggtcg ctttcgaaga ccagggagag
    49681 atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg gctggcgagt
    49741 gccgggccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt taacggcgca
    49801 ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag cctggcagcc
    49861 tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga accacgcaga
    49921 atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg ggaaacgggt
    49981 gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa aatcaccatc
    50041 gtgaattagc aacgcgtgcc gccaatggct gtaataagtt gccatctggc gcaggtttac
    50101 gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacattttt tcaagctggg
    50161 acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta tctgttcatc
    50221 ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg gtttggtaaa
    50281 gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag caccattcat
    50341 actcccctga cgtataacga aatcaacggg aaaccttttg gcctggatta cgaactggcg
    50401 aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc
    50461 cagctgtttg acgacctcga taatggtaac gccgacctgc tggcggcagg acttgtctat
    50521 aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt gtcacaacaa
    50581 ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa
    50641 ctcaccgttg caccgggtca tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa
    50701 ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt aatggaagat
    50761 gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag cctgtttcag
    50821 cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc ggtgacctgg
    50881 tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt cttcaacgaa
    50941 atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca tggcgatgat
    51001 tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact gccgcagtta
    51061 aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc cgctattgct
    51121 tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg
    51181 atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga tgccgaacag
    51241 agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg
    51301 ccggagaacg agcggatctg gtttgccctc gctgcgtaca atatgggcta tgcgcatatg
    51361 caggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta
    51421 aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac ttacggctac
    51481 gctcgtgggc atgaagccta cgcttatgtc gaaaatattc gtaagtatca gattagcctg
    51541 gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca actggcgcag
    51601 gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt tctctcgttt
    51661 ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt ttccaggaaa
    51721 gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg cgctttaatt
    51781 tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc cgccagtatt
    51841 ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc cattaaagat
    51901 cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg
    51961 atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt ggcgtcgatc
    52021 agacgataat tttgcatcac cagaccgccc tgcctcaggg ccatgatttc tgcatgtgcg
    52081 gtgggatcat gtcgaccaat cggacggttc cagccttcgc caataacacg attgttatgc
    52141 actaataccg cgccgaccgg tacttcccgc tcatcccagg cacgcttcgc cagcgtcatt
    52201 gcatgacgca tccagtattc gtggctaaat tcgacttcag acaaaaagaa aactccggtt
    52261 ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag ttgctggagt
    52321 tcaccgcgcg gggttactcg ccagcgatgc tggcaaaagt aaagcagcgg gttgtcctga
    52381 ttactgtcgc tatagccact gtacagccgc agcggcgtgc cgattttgcg ctccagttgc
    52441 gcgacctttt catgcccgag gcaacgcatc gtcaataccc aaccaccata gccacgctga
    52501 atttggctgg cgataagatt cacccgcggc agccagggtg tatcgaaata gaccgcttca
    52561 accagcggct gtggagaacc ggtaatcaac cagatatcag catcggaact taacaggtag
    52621 gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcacgaaa ccagcgtacg
    52681 aaatcggcct gcaacgcctg taaacgtgct tcgctgtgac caaaggtgca tccccacaga
    52741 agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat ggctataatc
    52801 ggcaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa atagcgcaga
    52861 aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa caccacacga
    52921 cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta attttcgcta
    52981 ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc atcatggcta
    53041 actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg
    53101 tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa cggcttactt
    53161 cgtatccgtc agcgttacca ggggcttgcc cagagtgata aaaaactggc ggattatctg
    53221 ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa cgaagccgga
    53281 gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg ttttccggcg
    53341 cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc agtgcccatt
    53401 cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact gattaaagaa
    53461 aatactgccg ctatgtacgc aacgctaaac gttaatagtg aagagaaact gcatgaatgc
    53521 gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc ttcgggtctg
    53581 gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc cgcagtgcgc
    53641 gatatgcatg cgctactcgc taccgtacag gcgtcgtccc ctgacgatct gttattagcc
    53701 atttcttaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat gctgcgagtg
    53761 ggcggaaaag tgctggcgat taccggcttt actcccaatg ccctgcaaca gcgtgcttct
    53821 cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt
    53881 cacgcgcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca ggatctggaa
    53941 ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag
    54001 aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta tggcgctgtt
    54061 aatcactaaa aaatgcatca attgtgatat gtgtgaaccc gaatgcccga atgaggcgat
    54121 ttcaatggga gatcatatct atgagattaa cagcgataag tgtaccgaat gcgtagggca
    54181 ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc aatactattg tgaaagatcc
    54241 gcaacatgtc gagacagaag aacagttgtg ggataaattt gtgctgatgc accacgcgga
    54301 taaaatttaa
//
LOCUS       DAZFKY010000006        50757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFKY010000006 DAZFKY010000000
VERSION     DAZFKY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..1330
                     /locus_tag="ACHKCL_000336"
     CDS             95..1330
                     /locus_tag="ACHKCL_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5761379.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREIARQWALAPLP
                     QSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGTPWSEVPDNFL
                     CPECSLGKDVFDELASEAK"
     gene            1327..2460
                     /gene="norW"
                     /locus_tag="ACHKCL_000337"
     CDS             1327..2460
                     /gene="norW"
                     /locus_tag="ACHKCL_000337"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5761380.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(2588..4840)
                     /gene="hypF"
                     /locus_tag="ACHKCL_000338"
     CDS             complement(2588..4840)
                     /gene="hypF"
                     /locus_tag="ACHKCL_000338"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5761381.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(4993..5520)
                     /gene="hydN"
                     /locus_tag="ACHKCL_000339"
     CDS             complement(4993..5520)
                     /gene="hydN"
                     /locus_tag="ACHKCL_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5761382.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5669..6679)
                     /gene="ascG"
                     /locus_tag="ACHKCL_000340"
     CDS             complement(5669..6679)
                     /gene="ascG"
                     /locus_tag="ACHKCL_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO5761383.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            6939..8396
                     /gene="ascF"
                     /locus_tag="ACHKCL_000341"
     CDS             6939..8396
                     /gene="ascF"
                     /locus_tag="ACHKCL_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO5761384.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            8405..9829
                     /gene="ascB"
                     /locus_tag="ACHKCL_000342"
     CDS             8405..9829
                     /gene="ascB"
                     /locus_tag="ACHKCL_000342"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO5761385.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(9953..10423)
                     /gene="hycI"
                     /locus_tag="ACHKCL_000343"
     CDS             complement(9953..10423)
                     /gene="hycI"
                     /locus_tag="ACHKCL_000343"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5761386.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(10416..10826)
                     /gene="hycH"
                     /locus_tag="ACHKCL_000344"
     CDS             complement(10416..10826)
                     /gene="hycH"
                     /locus_tag="ACHKCL_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5761387.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(10823..11590)
                     /gene="hycG"
                     /locus_tag="ACHKCL_000345"
     CDS             complement(10823..11590)
                     /gene="hycG"
                     /locus_tag="ACHKCL_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5761388.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(11590..12132)
                     /gene="hycF"
                     /locus_tag="ACHKCL_000346"
     CDS             complement(11590..12132)
                     /gene="hycF"
                     /locus_tag="ACHKCL_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5761389.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(12142..13851)
                     /gene="hycE"
                     /locus_tag="ACHKCL_000347"
     CDS             complement(12142..13851)
                     /gene="hycE"
                     /locus_tag="ACHKCL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5761390.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(13869..14792)
                     /gene="hycD"
                     /locus_tag="ACHKCL_000348"
     CDS             complement(13869..14792)
                     /gene="hycD"
                     /locus_tag="ACHKCL_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5761391.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(14795..16621)
                     /gene="hycC"
                     /locus_tag="ACHKCL_000349"
     CDS             complement(14795..16621)
                     /gene="hycC"
                     /locus_tag="ACHKCL_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5761392.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(16618..17229)
                     /gene="hycB"
                     /locus_tag="ACHKCL_000350"
     CDS             complement(16618..17229)
                     /gene="hycB"
                     /locus_tag="ACHKCL_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5761393.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(17354..17815)
                     /gene="hycA"
                     /locus_tag="ACHKCL_000351"
     CDS             complement(17354..17815)
                     /gene="hycA"
                     /locus_tag="ACHKCL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5761394.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18027..18377
                     /gene="hypA"
                     /locus_tag="ACHKCL_000352"
     CDS             18027..18377
                     /gene="hypA"
                     /locus_tag="ACHKCL_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5761395.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            18381..19253
                     /gene="hypB"
                     /locus_tag="ACHKCL_000353"
     CDS             18381..19253
                     /gene="hypB"
                     /locus_tag="ACHKCL_000353"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5761396.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            19244..19516
                     /gene="hypC"
                     /locus_tag="ACHKCL_000354"
     CDS             19244..19516
                     /gene="hypC"
                     /locus_tag="ACHKCL_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5761397.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            19516..20637
                     /gene="hypD"
                     /locus_tag="ACHKCL_000355"
     CDS             19516..20637
                     /gene="hypD"
                     /locus_tag="ACHKCL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5761398.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPAVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            20634..21644
                     /gene="hypE"
                     /locus_tag="ACHKCL_000356"
     CDS             20634..21644
                     /gene="hypE"
                     /locus_tag="ACHKCL_000356"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5761399.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            21718..23796
                     /gene="flhA"
                     /locus_tag="ACHKCL_000357"
     CDS             21718..23796
                     /gene="flhA"
                     /locus_tag="ACHKCL_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5761400.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(23833..24186)
                     /gene="ygbA"
                     /locus_tag="ACHKCL_000358"
     CDS             complement(23833..24186)
                     /gene="ygbA"
                     /locus_tag="ACHKCL_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5761401.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(24263..24397)
                     /locus_tag="ACHKCL_000359"
     CDS             complement(24263..24397)
                     /locus_tag="ACHKCL_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761402.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            24473..27034
                     /gene="mutS"
                     /locus_tag="ACHKCL_000360"
     CDS             24473..27034
                     /gene="mutS"
                     /locus_tag="ACHKCL_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5761403.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            27140..27796
                     /gene="pphB"
                     /locus_tag="ACHKCL_000361"
     CDS             27140..27796
                     /gene="pphB"
                     /locus_tag="ACHKCL_000361"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5761404.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(27837..28073)
                     /locus_tag="ACHKCL_000362"
     CDS             complement(27837..28073)
                     /locus_tag="ACHKCL_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5761405.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(28084..29511)
                     /locus_tag="ACHKCL_000363"
     CDS             complement(28084..29511)
                     /locus_tag="ACHKCL_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5761406.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(29511..30104)
                     /locus_tag="ACHKCL_000364"
     CDS             complement(29511..30104)
                     /locus_tag="ACHKCL_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5761407.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            30251..30658
                     /locus_tag="ACHKCL_000365"
     CDS             30251..30658
                     /locus_tag="ACHKCL_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5761408.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(30778..31770)
                     /gene="rpoS"
                     /locus_tag="ACHKCL_000366"
     CDS             complement(30778..31770)
                     /gene="rpoS"
                     /locus_tag="ACHKCL_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5761409.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(31833..32927)
                     /gene="nlpD"
                     /locus_tag="ACHKCL_000367"
     CDS             complement(31833..32927)
                     /gene="nlpD"
                     /locus_tag="ACHKCL_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5761410.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(33112..33738)
                     /gene="pcm"
                     /locus_tag="ACHKCL_000368"
     CDS             complement(33112..33738)
                     /gene="pcm"
                     /locus_tag="ACHKCL_000368"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5761411.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(33732..34493)
                     /gene="surE"
                     /locus_tag="ACHKCL_000369"
     CDS             complement(33732..34493)
                     /gene="surE"
                     /locus_tag="ACHKCL_000369"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5761412.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(34474..35523)
                     /gene="truD"
                     /locus_tag="ACHKCL_000370"
     CDS             complement(34474..35523)
                     /gene="truD"
                     /locus_tag="ACHKCL_000370"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5761413.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(35520..35999)
                     /gene="ispF"
                     /locus_tag="ACHKCL_000371"
     CDS             complement(35520..35999)
                     /gene="ispF"
                     /locus_tag="ACHKCL_000371"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5761414.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(35999..36709)
                     /gene="ispD"
                     /locus_tag="ACHKCL_000372"
     CDS             complement(35999..36709)
                     /gene="ispD"
                     /locus_tag="ACHKCL_000372"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5761415.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(36728..37039)
                     /gene="ftsB"
                     /locus_tag="ACHKCL_000373"
     CDS             complement(36728..37039)
                     /gene="ftsB"
                     /locus_tag="ACHKCL_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5761416.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(37233..37556)
                     /gene="ygbE"
                     /locus_tag="ACHKCL_000374"
     CDS             complement(37233..37556)
                     /gene="ygbE"
                     /locus_tag="ACHKCL_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5761417.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(37606..38211)
                     /gene="cysC"
                     /locus_tag="ACHKCL_000375"
     CDS             complement(37606..38211)
                     /gene="cysC"
                     /locus_tag="ACHKCL_000375"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5761418.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(38211..39638)
                     /gene="cysN"
                     /locus_tag="ACHKCL_000376"
     CDS             complement(38211..39638)
                     /gene="cysN"
                     /locus_tag="ACHKCL_000376"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5761419.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(39640..40548)
                     /gene="cysD"
                     /locus_tag="ACHKCL_000377"
     CDS             complement(39640..40548)
                     /gene="cysD"
                     /locus_tag="ACHKCL_000377"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5761420.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            40800..41837
                     /gene="iap"
                     /locus_tag="ACHKCL_000378"
     CDS             40800..41837
                     /gene="iap"
                     /locus_tag="ACHKCL_000378"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5761421.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   41919..42131
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=41980..42009
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(42227..42520)
                     /gene="cas2e"
                     /locus_tag="ACHKCL_000379"
     CDS             complement(42227..42520)
                     /gene="cas2e"
                     /locus_tag="ACHKCL_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5761422.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(42517..43440)
                     /gene="cas1e"
                     /locus_tag="ACHKCL_000380"
     CDS             complement(42517..43440)
                     /gene="cas1e"
                     /locus_tag="ACHKCL_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5761423.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(43437..44087)
                     /gene="cas6e"
                     /locus_tag="ACHKCL_000381"
     CDS             complement(43437..44087)
                     /gene="cas6e"
                     /locus_tag="ACHKCL_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5761424.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(44069..44815)
                     /gene="cas5e"
                     /locus_tag="ACHKCL_000382"
     CDS             complement(44069..44815)
                     /gene="cas5e"
                     /locus_tag="ACHKCL_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5761425.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(44826..45881)
                     /gene="cas7e"
                     /locus_tag="ACHKCL_000383"
     CDS             complement(44826..45881)
                     /gene="cas7e"
                     /locus_tag="ACHKCL_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5761426.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(45893..46429)
                     /gene="casB"
                     /locus_tag="ACHKCL_000384"
                     /gene_synonym="cse2"
     CDS             complement(45893..46429)
                     /gene="casB"
                     /locus_tag="ACHKCL_000384"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5761427.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(46426..47988)
                     /gene="casA"
                     /locus_tag="ACHKCL_000385"
                     /gene_synonym="cse1"
     CDS             complement(46426..47988)
                     /gene="casA"
                     /locus_tag="ACHKCL_000385"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5761428.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(48086..>50757)
                     /locus_tag="ACHKCL_000386"
     CDS             complement(48086..>50757)
                     /locus_tag="ACHKCL_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO5761429.1"
                     /translation="DKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVK
                     RNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRK
                     NYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRI
                     DELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLAD
                     WTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHP
                     TPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMA
                     TANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTF
                     SQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHAC
                     DAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDD
                     YPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNS
                     VDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVL
                     ICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPEL
                     LILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIES
                     VYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLA
                     EESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCI
                     EQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        12039 a        13963 c        13119 g        11636 t
ORIGIN      
        1 tttatagagt aaaaacaatc agataaaaaa ctggcacgca atctgcaatt agcaagacat
       61 ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga aaaataacat
      121 tcattgggtt ggtcaacgtg actgggaagt acgagatttt cacggcacgg aatataaaac
      181 gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg tgctgatcga
      241 caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct
      301 ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgccg gggcgctgac
      361 cgaactgatg gcacaaattc ccgatacgcc gatctactgt acagccaacg ctatcgactc
      421 gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa ctggcgacac
      481 gctggatatc ggcaacggca aacagctcat ttttgtcgaa acaccaatgc tgcactggcc
      541 ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg
      601 tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg agctttttga
      661 gcagtgccag cgttactacg ccaatatcct tacgccgttc agccgtctgg taacgccgaa
      721 aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct ctcacggcgt
      781 ggtatggcgc gataacccga cgcaaattgt cgagctgtac ctgaaatggg cggctgatta
      841 tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc gcatgatggc
      901 tgacgctatc gcccagggga ttgcggaaac cgacccacgc gtggcggtgg atcgtctttc
      961 cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcga aatggcgacc
     1021 agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
     1081 gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac
     1141 cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
     1201 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
     1261 taacttcctg tgcccggaat gctccctcgg caaagacgtc tttgacgaac tggcatcgga
     1321 ggcaaaatga gcaacggcat tgtgatcatt ggttcgggct tcgccgcccg ccaactggtg
     1381 aaaaatattc gcaaacagga cgccagtatt ccattaaccc tgattgccgc cgacagtatg
     1441 gatgagtaca acaaacctga cctcagccat gttatcagtc aggggcaacg tgccgacgac
     1501 cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgctcct gtttccgcaa
     1561 acctgggtga cggatatcga tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg
     1621 caatacgaca agctggtgct ggcaaccggt gccagcgcct ttgtcccgcc agtgcctggg
     1681 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
     1741 cgggatgccc gacgcgtgtt gattgttggc ggtggtttga ttggtagcga actggcgatg
     1801 gatttttgtc gtgcaggcaa aatggttacg ctaatcgaca acgctgccag tattctggcg
     1861 tcgttaatgc caccggaagt aagcagtcgc ttgcagcatc ggttgacgga gatgggcgtt
     1921 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
     1981 acgctggacc accagcgcag catcgaagtg gatgcggtaa ttgccgccac cggactgcgc
     2041 ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
     2101 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
     2161 ggtcaggtat tgccgttcct gcagccgatt caacttagcg cgatggtgct ggcaaaaaat
     2221 cttctcggca ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg
     2281 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat
     2341 accgaacgcc agggaatggt ggcgcgcggt gttgacgatg cagaccaact tcgcgccttt
     2401 gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacgtt gccggtgtag
     2461 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg
     2521 gacgcatgtg taggccggat aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca
     2581 acacgtttta tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct
     2641 gccccagaga caaaccgcca tcacccgccg gtaaactctg tggaaacagc aatgtgaaat
     2701 cagcgagata atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa
     2761 ataccagcgt agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct
     2821 gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggtgcctgc cagttcagcc
     2881 actgctgcca gaaagtggcg agatccagtt gattgtccac ccgcggcatc gtcaccggat
     2941 gcgtcactcc gtcgcatgag gctgctagcg cctccagagc acaagccgct tcaccttcat
     3001 aacttaacgt ggctggcgca cagcccagtg ccgccgccac agcatcgaac aaacgcccac
     3061 acgatgacgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagt acgctccagt
     3121 tttgctgttg cacacttgcc gtttcagggt aattctgcca ctccggcaca aagcgcaggc
     3181 actgcgccag caggttacgc cacggctgct tcgccgccaa atcgccaccc ggaagcgcca
     3241 ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc
     3301 cccacaaagc gccgttctcc cccataccga taccgtcgag cgtcaaagca atgacatcac
     3361 cgccatccag cggccactga tgctctgcca gacacgccgc tgcgtgggca tgatgatgca
     3421 gcaccgtttg cgtcggcaga ttcatttcgc tcgcccactg gcaggagaca tagcccggat
     3481 gtgcgtcatg cacaacgtat tgcggagtga aattgtagat gttttgcatc aggcgtaacg
     3541 cttcgcgcca ctgcgtctgg atgccatcgt cacttaaatc gcccagatgc tggctcaaca
     3601 ccacttgttc accgcgcacc aggcagaagg tatttttcag atccgcgccg agacacagta
     3661 caggcggaac atttttaaag cccggaggca aagccagcgc atccggcaca tacccccgcg
     3721 aacggcgcag catttcgccg ctttcgcgca ccaccgaatc atccatccgc tgcacaatgt
     3781 cgcggttgtg tattaagaat ccgtcggcaa tgccctgcaa atcctccagc gcctgttcgt
     3841 tgctgatagc cggtggttta ccgctcaggt tgccagaggt catcaccagc gggcattgta
     3901 gttcctgtaa cagcaaatgc tgaagcggat tcgcaggcaa cattaccccg acttcgttaa
     3961 ggccaggggc gatatcatca caaagctcag gaacgtattt tttatccacc agcacaatcg
     4021 gcgcggcggg cgtggtaagc aactggcgcg cagcgtctgg taaaccgtca gccactggca
     4081 acatgactgc cagcggtttc gccgggcgat gtttgcgcgc ccgaagtgtc gccaccgcgt
     4141 tactgttacg tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt
     4201 tgcccatttt taactgtgcg atagccgcct gtaatgccgc ctcttgttct gcatgttcac
     4261 catgacttac ccattcaaga tacgggccac actccgggca ggccaccggc tgggcgtgga
     4321 agcgacgatc gagcgggtcg cggtactctt tatcacaggc tggacacagc ggaaacgccg
     4381 ccatcacggt aaacgggcga tcgtaaggca tggcgcgaat aatggtgaag cgcgggccgc
     4441 agtgggtaca gttgataaat ggataacgat aacgccgttc gcctggggta ttcatttcgg
     4501 caaggcaagc agggcaagtg gcggcatcgg gaacaatttg cgtattcatg gcgccgcccg
     4561 cgctctggcg gatggtgaac tcagtgggca gttgtgacca gataaacggt tcacgctcaa
     4621 cgctatcaat acgcgccagc ggcgggcagt gctgatgcaa ttgaacaaga aacgtttccg
     4681 ggtcttccag cagccggact tctacgccat cgccgtcatt acagacatcg ccgtgaagat
     4741 ttaattgctg tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgcctc
     4801 gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat
     4861 gaattgcgta acccgtccag ccggacacga cagcgtcgca tccggcagtc acaggtcggc
     4921 gataccgccc gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt
     4981 tctgtaagat ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc
     5041 aagtttattg cgatcgacac aaattagcgc atgggtcggg caagccgcca tacacgccgg
     5101 gccgtcttca cgatggttgc acaggtcgca tttattggct tcggctttgt cagcccgtac
     5161 attcagccct gcgccgctgt tgcgaatcac cggacgtacc accacttcca tcgcgccata
     5221 cgggcaagcc acaacgcagg ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa
     5281 ccctttatca cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca
     5341 ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa
     5401 aaatgtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc
     5461 cacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat
     5521 caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg ccagttttta
     5581 atttgctgtt atttaaggaa attaatttct gtaatgcagg aaaaaagatg tcatcgacac
     5641 tagtgacgat gacatgtgat gacaatgttt atcgcgaaag agcaatgagt gagtcgcggc
     5701 ggatcagttt tccgctgaag gttttcggcg gtgagaaatc cccgccatcg agcataaaaa
     5761 tcagccgtcc aataatttcc tgaatcatct cagtcaccgg aatttttacg ctggagagcg
     5821 ccggaacgat atagggggca attgcgatat catcgaatcc gataactgac acctgctctg
     5881 gcaccgctac gccgcgcttg tgtaacgctt tcatcgcacc tatcgccata tcgtcgttac
     5941 tggcaactaa cgcgctaaat ttagccccac gttcgagcaa cgtttctacc ccttcggccc
     6001 cgctggcggg cgtccattta ccgttagcga taagtttttc attgagcgca ataccatgct
     6061 gcgccagcgc gtctttatat ccggcaagac gttcaacgct ggtgggggaa tccatcgagc
     6121 cggtaagaaa agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc
     6181 tggtctgttt atgatcgcac cagacgctat ggctgctatt tttgcgcagg cggcgattaa
     6241 gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc gatctcatcc acacttaaaa
     6301 aacgcgggta aatcataatg gcgtcgcagc gcaaatccag cagatactga atcgcctggc
     6361 gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa ctgccgccct ttctcttccg
     6421 ccattcgcgc ggcatgaaac agtaattcac taaaataaat gccgtggtaa agcgtgttag
     6481 tcactaccag ccccagcgtc tgggtacttt tcgccgacag attgcgcgcc agcaagtttg
     6541 gacggtaacc gctctcttct accgcctgaa acacgcgatc tttagtctcc tggctgacgt
     6601 agccattacc tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca
     6661 cttccagcat cgtcgtcatc attttcatcc ctttacacgc aatcaacgca gtgtactgca
     6721 ccgtttaccg attgtccttg cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca
     6781 aatataaaaa atgaacaatt aactctcttg cttatttggt tacaactatt catgatttta
     6841 tgaaaccggt ttcttaattc cgtttcagca tcggcatttt tccgtcacgt cgactgataa
     6901 caactacatc ttccctactg ataacaggat aaaatccgat ggccaaaaat tatgcggcgc
     6961 tggcacgctc ggtgatagcg gcactgggcg gcgttgataa catctcggcg gtcacgcact
     7021 gtatgacgcg tttgcgcttt gttatcaaag atgatgcgct tatcgacagc ccgacgttaa
     7081 aaaccatccc cggcgtgctc ggcgtggtac gtagtgacaa ccagtgtcag gtgattattg
     7141 gcaataccgt ttcacaagcc tttcaggaag tcgtcagcct gctgccggga gatatgcagc
     7201 ccgccctgcc cgtgggtaaa cccaaactca cgctgcgtcg cattggtgcg gggatcctcg
     7261 atgcgctgat cggcaccatg tcaccgctga tcccggcgat tatcggcgga tcgatggtca
     7321 aactgctggc gatgatcctc gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa
     7381 ccattctgaa tgtgattggt gacggtgctt tcttcttcct gccgctgatg gtcgcggctt
     7441 cggccgccat caaatttaaa accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg
     7501 tacatccgag ttttattgaa ctgatggcga aagcggccca gggtgaacat gtcgaatttg
     7561 ccctgattcc agtcaccgcg gtgaaataca cctacacagt gatcccggcg ctggtgatga
     7621 cctggtgcct gtcatatatc gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa
     7681 acttcctcaa gccaatgctg attgtgttga ttgccgcacc gctggcaatc ctgctgattg
     7741 gcccgattgg tatctggatc ggtagcgcca tttcggcgct ggtttacacc attcatggtt
     7801 atctgggctg gctttcagtc gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg
     7861 ggatgcaccg cgtctttacg ctaaccatca ttcagaccat tgccgaaacc ggcaaagaag
     7921 ggatggtcat gccgtcagag atcggcgcta acctgtcgct gggcggttca tcactggcgg
     7981 tggcgtggaa aacgaaaaac ccggaactgc gccagactgc gctggctgcg gcggcatcag
     8041 ccattatggc ggggatttcc gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc
     8101 cgcttatcgc cagtcttatc agcggtttta tttgcggcgc ggttgccggt atggcggggc
     8161 ttgccagcca ctcaatggca gcgccggggc tatttaccag cgtgcagttc ttcgatccgg
     8221 cgaatccaat gagcatcgtc tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt
     8281 ttatcctcac actgttgctc ggctttgagg atattcctgt tgaggaagcg gctgccgagg
     8341 cgcgaaagca tcagagcgca caaccgaccg tcgccaaaga agtaagtctt aattgaggat
     8401 gaaaatgtca gtatttccag aaggtttttt atggggcggc gcgcttgccg ccaaccagtc
     8461 tgaaggtgcg ttccgtgaag gtggcaaagg actgacaacg gtcgatatga tcccacacgg
     8521 cgagcatcga atggcggtga aactggggct ggaaaaacgt tttcagttgc gcgatgacga
     8581 gttttatccc agccatgagg cgacgggttt ttatcatcgt tataaagaag atatcgccct
     8641 gatggcagag atgggattca aagtgttccg tacctcaatt gcctggagcc gccccttccc
     8701 gcagggtgat gaactgacgc ccaaccagca gggcattgct ttttaccgtt ctgtctttga
     8761 agagtgtaaa aagtacggta tcgaaccgct ggtcacattg tgccacttcg atgtgccgat
     8821 gcagctggtc accgaatatg gctcctggcg taaccgcaag ctggtggagt ttttcagccg
     8881 ctacgccagg acctgctttg aagcatttga tggtctggtg aaatactggc taaccttcaa
     8941 tgaaatcaac attatgttgc atagcccgtt ctccggcgcg gggctggtgt ttgaagaagg
     9001 tgaaaatcag gatcaggtga aatatcaggc cgcgcatcac cagctggttg ccagtgcgct
     9061 agccaccaaa atcgcccatg aggttaaccc gcaaaatcag gtgggctgta tgctggcggg
     9121 cggtaacttc tacccttaca gttgcaagcc ggaagatgtc tgggcggcgc tggagaaaga
     9181 tcgggaaaac ctgtttttta tcgatgtgca ggcgcggggc gcgtatccgg cttactctgc
     9241 tcgcgtattc cgcgaaaaag gggtaaccat cgacaaagca ccgggcgatg atgagatcct
     9301 gaaaaacacc gtcgattttg tctctttcag ctattacgcc tcgcgctgcg cctcggcgga
     9361 gatgaacacc aacaatagca gtgcggcgaa cgtagtgaaa tcgctgcgta acccgtatct
     9421 acaggtgagc gactggggct ggggaattga tccactcggt ctgcgtatca ccatgaacat
     9481 gatgtacgac cgctatcaga agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga
     9541 tgaacttgct gccaatggcg agattaacga tgactatcgc atcagctatt tacgcgaaca
     9601 tatccgcgca atgggcgaag cgattgcaga tggcattccg ctgatgggct acaccacatg
     9661 gggctgtatt gatttagttt ccgcctctac gggtgaaatg agcaaacgct acggttttgt
     9721 ctttgttgac cgtgacgacg caggcaacgg cacgctgacg cgcacacgta agaaatcgtt
     9781 ctggtggtat aaaaaagtga ttgccagtaa tggggaagat ttagagtagg taacctactg
     9841 tcgcactaac caggaaggcc cgataagcgt tgcgcatcgg gcaatctggg gtttgctatc
     9901 agttctgatt ccctcatcaa gaacatccct gtcctgatta cttattgaaa aactactctt
     9961 cttccaccgc taactgcgcg aagccgccgt tcccttccca gccttccagt cgttgataaa
    10021 cggtttctac cgcatcttta atcggctggg tcatcgggta gtaaaagccg acgatatccg
    10081 gctggatgcc gaggaaaatc acttcgccaa tatcttcttt caactgatcg ataaggtagt
    10141 tcaacggcat gttatgggta gtcatcataa acatctcggc gatatcatcc gggtcgatga
    10201 tgcggatttc gccggggttt agccccatat ccgtggcgtc gacaatcagc agccgtgtcg
    10261 ggcgcagttc gcggatagcg acaatgtcgt tttccggtgc gctaccgccg tcaatcacca
    10321 cccagttacc tttcggcgcg gcggcgcact tttccgccag cagcggacct gcgccatcat
    10381 cacccatcat gctattgcca acacagagta aaacgtcagt cacgcagtct cctcaccatc
    10441 aaatagatgg cgttttcctg atgaatatca tgcagcatac ttaacagcat tgtactccat
    10501 tcctgctgct gcgaagtttg cgctgcccgg gctttatcga atgcattggc cagcatcggc
    10561 acatggttaa tgtcgatgac gatctcacca tatttcggca caccttccat tttgcggcgg
    10621 gcttcgctgc ccgcctccag agtggcaatc catgccagat attcatccca cgggcaggtg
    10681 agcgccgctt ccaggcaatc gataacccca aggtggtgac caatcgccag gctgtaatag
    10741 accacctgct gcgcctcggc gggcgtggca tcgttctcat caataaattt acggctcagt
    10801 tgactgaaca ccaccttttc actcatcgga tacgcgcctc ttcaacaaca tgattcagat
    10861 ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc cagccagcgt gcaacctgtt
    10921 cttcgccctg ccctaactgt gtaaggtaat catcggcaat ctgacgaccg taacgataac
    10981 ccgccagtcg acgtgcttcg cgatccactt tcacacgcag cggctgcacc atatcgccat
    11041 gcaggatctc cgccggttgt tcatcctgct cacccggccc acgggcgtga attttctgct
    11101 ccagcaggcc gagcgccatt gcaaagccgt acagcgtggc ggctggcgtt ggcgggcagc
    11161 cagggatata aacatccacc gggacgattt tatccgtacc gccccacacg cagtagagat
    11221 cgtggaagat cccgccgctg ttaccgcagg caccgtagga gatacaaatt ttcgggtccg
    11281 gcgcggactg ccacgcacgc agcgcagggg atcgcattgc acgggtgacc gcgccggtaa
    11341 acagtaaaat atccgcatga cgcggtgaag gaacgacttt aatgccgaag cgttctgcat
    11401 cgaacagcgg cgacaaagtg gcgaaaattt cgatttcgca gccgttgcag ccgccgcagt
    11461 ccacgcggta aacgtaggca gaacgtttga tttttttcag taacgacgcc ttcatgctgg
    11521 cgatggattc atccaccgtc atcgggaccg gaatgccgtt ggcgtcacgg gggcctaata
    11581 aattgctcat cagatggcct ctttcatatg gcgagtcagt tcaatacggt cggacggcac
    11641 caggcatttc tggcgcttac attccgggca agtctcaaag ctttcgcggt ggttttccgc
    11701 gcggctgtcg ccattgtgct taagcagcgc aatggcgtag tcgatctctt tctggacggc
    11761 gaaaggacga ttgcagacgc ggcagttgca cagcgcgaag cgggactgtt gcaggaagtc
    11821 ttctttcttc cacaccgcca gttcgtactc ttgcgacagt ttgattgccg ccgtcgggca
    11881 gacttcttcg cagcgtccgc agaagatgca gcgcccgaga ttgaactccc acgccagctc
    11941 accggtggcg aggtcagttt caaccgttaa ggcgtttgac gggcaggcat tgacgcaggc
    12001 cgcgcagccg atgcactgct gcgggttctg ctctggctta ccacggaagt ttttatcaac
    12061 cgcaatcggt tccagcggat aagacgaggt cgccgtgccg gttttgatga cttttttgat
    12121 aaaggtaaac atggcgattc cttatttcag cggcgagttt ttacgctcaa tgctgtaacg
    12181 ctcgagttct ttgtacggca ccactttgct ctttttctta cgcacatcga ccacggtcat
    12241 gcggtcggta caggagtagc aagggtccag actaccgata atcagcggcg catcagaaac
    12301 ggtgttgccg cgcagcatgt agcgcagggt cggccagttc gcgtaggtcg cggcacggca
    12361 gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc cagtggatat catcgccgcg
    12421 cggcgcttcg gcaaagccca gcgcaaaacg gtgcggaatg taggtaaagc cttccaccat
    12481 cagcgggccg cccggcaggt tatccagacc gtagtcgatc atgttcagcg cggtatagac
    12541 ttcgttaata cgcactttca gacgcgaaat aacgtcgcag ccctgctcgc tgtggacttc
    12601 cattggcagc aggccataac caacaaacgg gtgatcggcg cgggtatcgc gggcgtgacc
    12661 gctggcacgg accatcgggc cgacgttact gaagtcgcga gcgatttccg ggtccagacg
    12721 accaatgccg acagtgcgct gttccatgtt cggcgtactc agcagcacat ccaccagctc
    12781 ctgcacttca cgacgcatct gttgcgccag ttgacgggtc tggatcatgt catctttcag
    12841 cagatcgcga cgaatcccgc cgatcaggtt caggccgtag gttttacgcg caccggtaag
    12901 gatctctgcc attttcatgg aggtttcacg cacgcggaag aactgcataa agccggagtc
    12961 aaagccggta aagtgacagg cgaggccgag gttgagcaga tgcgagtgca gacgttctac
    13021 ctccagcaga atggcgcgga tcatctgcgc acgttctggc acctgaatac ccatcgcgtt
    13081 ttccaccgac gtggtgtagg cggtgctgtg agcaaaaccg cagatcccgc acacacggtc
    13141 agagaggaag gtcacttcgt tataacccat gcgggtttct gccagttttt ccatgccgcg
    13201 atggacatag aacaggcggt agtcggcgtc gataatgttt tcgccatcga cgaacagacg
    13261 gaagtggccc ggttcgtcag aagtgacgtg cagcggacca atcggcacga cgttgttttt
    13321 cttgtcgccc agttcgttga tgaactcgta ggtttcagca tcggtggtcg gtgccggacg
    13381 ctgacgataa tccatgctgt ctttacgcag cggataaagt tcatccggcc agtcatccgg
    13441 cagcaccaga cgacgttcat ccggcaaacc aaccggaatc aaaccgtaca tatcacgtac
    13501 ttcacgctcg ccccacaccg cagccggaac gcgcggcgtc acggaaggat actccggttt
    13561 gttggcatca acttcgacgc gaaccgttac ccagcactta gtccccttct ccatcgacag
    13621 cacgtagtaa acggcgtaat gaccattcag tttgcgttcg tcgttaccaa aaagcaccga
    13681 cagccagccc ccctgcttgt agtagagaaa ctccaccact tccggcagat agttcacctt
    13741 tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg tccagcacga cgcccggaaa
    13801 tgcctcattc agcgcggcga gataatgttg acctaatttt tcttcagaca tgctcaaact
    13861 ctctttaatc acgccgccag caaggagacg aacgctaaaa atgcaaagcc aaacccagcc
    13921 caggtaatgc gcggagtaat atcaagacgc agacgggcca tgctgttttc gaacagcgcg
    13981 ataaccagga cgccgaccac cagtttgacg atggcaatca ccaacgccag cagcagccca
    14041 ccgacggtga aggtttccat ttgtccccac ggaataaaca ccccgacgaa catctgcagc
    14101 accaccaact gtttcaggct gataccccat ttcatgacgc caaagccgct gccgctgtac
    14161 tcagagagcg gaccttcctg caactcctgc tcggcttccg ccaggtcgaa cggcagtttg
    14221 cccatttcga taaaagtggc gaacgcacag gcacaaagcg ccagcaccaa cgggatgctc
    14281 tggctcagcg gccagtgata aacggtgtcg gtgatgttgc tgatgttggt ggaactggca
    14341 acctgtgcgg caacccacag accaagcagc agcatcggtt cgaccagcac gccaagcatc
    14401 gcttcacggc tcgcgccgat agcggtaaac ggactaccgg tatccagacc agaaatggca
    14461 aagaagaaac gcgcgatggc aaagagatac agtaaggtga tcaaatcacc cagttgcggc
    14521 agcggagaac cgacggtcac caccggcagc gcagtagcga tagtcagcat gacgcccacc
    14581 atcacatacg gcgtcaggcg gaacacccag ccggaggcat ccgggccaac gctctggcga
    14641 cccagcagtt tgataatgtc gcgatactcc tgcaacacgc ccggcccgcg acggttatgc
    14701 aagcgggcgc gcgccacgcg ggtaataccg gagagcagcg gcgcaacggc aaatagcacc
    14761 agcgcctgaa ttaacggata taaaacactc attctcaggc tcctcgtgaa acaataatca
    14821 ccaccagcac cgccagttca accagcgcca tccggcggaa cagcaacgca ctgccttcgc
    14881 actgccagcc cggcaccagc gacaccggat tcagccattt gcgcagtttc agcaccggcg
    14941 caaacgcctg tttcaccggc atggcaaaac cgtgggcggt aattaccatt gatttttcat
    15001 ggtcgtagcc gcacacccag gccgcgccgc gggaacgcga tggcaaacga tcgcctttgc
    15061 aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat cagcagcaac gtgatcatcg
    15121 gttgagaaac ggtggtgtta gcaggttcca gcggcagagg tacagcagca gagagcatcg
    15181 gcagcagcca cggcgcagca acaccgccaa ttacgcagca aatcgccagt gccactacgc
    15241 ttacgctcat caggagcggt gcacaggtgg cgttttcggc ttctttggta cgcggcgcgc
    15301 cgaggaacgt gacgccatag actttcgcca tacacatcac cgccagcgca ccggtaattg
    15361 ccagacccac agcgagcagc ggccccagca gacgggcaac aaacgcgcca ctattgctca
    15421 gtttgaagaa ggattgatag ataacccatt ccccggcaaa accgttcagc ggcggcagtg
    15481 cagccattgc catcagcccg actaacatag cgatggagat aaccggcatt ttcttgccaa
    15541 taccaccgag tttttcgata tcgcgatgac cggtacggaa ccagacgctc cccgccccga
    15601 ggaacagtac gcttttgaac aggctatggt taagcagatg gtacagacca ccgaccaggc
    15661 caagagcaat cagcgccggt tgttcgagcg cgatacccgt tacgccagcg cccagcccca
    15721 gcaggataat gccgatattt tccagggtgt ggtaagccag tagacgctgg atattatgtt
    15781 ccatcaacgc atacagaccg ccgacaaacg cggtgatcat accgagcacc agcagcgcga
    15841 tcccccacca cagcggtgca ttaccgccca gcagtgacag ggttaaaatg cccagcaggc
    15901 caattttcat gactaccgta gaaaacaacg cggcagctgg cgcagaggcg ttcgcatgtg
    15961 cctgcggcac ccagccgtgc agcggaataa tcccggccag caggccaaag ccaatcacac
    16021 tgagcagcca gatatcggaa ccgagcggca gctgttgcat acgcatatcc agcaggcgca
    16081 gatccagcgt gccgtaacgc tgccacagca gccagcaagc aatcgccagc agcagagtgc
    16141 caagacgccc cagcgcaaac cacagtttgc cctctttgct gttgctggtg aggaacaccg
    16201 cacacaaggc catgacttcg gccattacca cgaacatgcc gaggttgctg gcaatgacgg
    16261 cgcagacggc ggcagccatc aacatattga tctgcaagcc gttgcacttc acctgcgcgt
    16321 ggcgatgcca gtcaatgttg tagaggctga caaacagacc gcacagaccg agcgtaatca
    16381 gccaaatcgc gttaagcgga gagatttgca catcgtagct taccagcgac agcgcaccgc
    16441 tcacgccaac cgcgccagtc agtacagtga agcccgcggc tgccgtatac agactaccaa
    16501 ccgcgccgcc aattccggct atccagccgc ttagcgcctt ttgaaaagaa aagagaaatg
    16561 ccagaacagc ggcggcgaca aaccacgcca cgccgctatt gatcagggaa attgcgctca
    16621 tttagcctct ccactttgag cctgctgaaa caaggtgaga tcgccaaagt ccgtgttaaa
    16681 ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg gtgttatcca ccagatgcag
    16741 ggctttagtc gggcacatcc gcacgcaggc cggaccttgt tcatcaaagc tacaaaggtc
    16801 acatttgacg gcgatcgcgc gaatacccgg cacccagtca agcagcgtac tgatacgcgc
    16861 cggagcaggc ggtgccggtg gcgctttcgg ggtattggca tttgcaggaa tatccagcgg
    16921 acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg atgccgcaca gcttgcagct
    16981 tacgcacagg ctttcattca actgcacggc cccatcaacg cgggtgatgg cgttaaccgg
    17041 gcagaccacc gcgcagggtg catcttcaca gtggtgacag agctgcggcg cagattcttt
    17101 ttcatttagc atcactctca ggcgcggcat tgattgcagg ccgtgctggc gatgcgtctc
    17161 tgaacaggcg gcctcacaag tgtggcaacc gatacagagc gtggagtcag caattacaaa
    17221 acgattcacc aggcattcct caggtgattg tcatttttga cgaaaacatg ccgatgaaat
    17281 gtcattttcg acactcatcg acacgcccat ccccaaacag gcgtaacgcc tgcaaaacgg
    17341 gcaaagcctc agctcatgct gccgggcttt gtccctttac cagttggctt aaattcaccg
    17401 gcacattgtt ttcaatagcg gtgtataagc tgtcgtaaac gccagcgatt ttttcgagat
    17461 agtgttccag aacctgttcg cgatcgcgga tgttgacctt gccatcaatc atgccgtcaa
    17521 tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc atcttttgtt tcgacgtaat
    17581 actcgatggt gtggctgtta aagccatccg ccagggtgac gtaaatacga aaatgttcaa
    17641 aaaggacgaa acagagttct ttgtccggcg cgcagctcat ggcgtgatgc aggcgcgctt
    17701 tcgataacgt gatcccctga accagcgaat tgcagtagat gtgccactgg tcctgtaggc
    17761 gacgatgccg ctgtgcgatg taatcggctt tctcgcttat ttcccaaata gtcattgtca
    17821 ggttacccgt ttaacagaga tgccagcttt aagcattttt tgtgccaact tttaatttat
    17881 tattattaaa gcgattttta agattttggt gaatttcgtc gccgtgtcga cgtgtcattt
    17941 cgacatcatc gacattattc actgcaggga taatcaacac tggcacaatt attgcttgta
    18001 gctggcaata gttaatggga ggcgatatgc acgaaataac cctctgccaa cgggcactgg
    18061 aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg cgtaactggg gtctggctca
    18121 aaattggcgc attttcttgt gtcgaaacca gctctcttgc cttttgtttt gatctggttt
    18181 gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga agaacaagag gccgaatgct
    18241 ggtgtgaaac atgccaacag tatgtgacgc tactgaccca gcgcgtccgc cgctgtccac
    18301 agtgtcatgg tgacatgcta cagattgtgg cagacgacgg tttacagatt cggcggatag
    18361 aaatagacca ggagtgagcg atgtgtacaa catgcggttg cggtgaaggc aacctgtata
    18421 tcgagggtga tgaacataac cctcattccg cgtttcgtag cgcgccattt gccccggcgg
    18481 cacgcccgaa gatgaaaatc accggcatta aagcgcctga atttaccccc agccagactg
    18541 aagaaggcga cctgcattac ggtcatggcg aagcgggcac tcacgcaccg ggcatgagcc
    18601 agcgtcggat gctggaagtc gaaattgacg tgctggacaa aaataaccgt ctggctgaac
    18661 gcaaccgcgc gcgctttgct gcctgcaagc aactggtgct caacctggtt tccagcccag
    18721 gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg cctgaaagac agcgttccgt
    18781 gcgcggttat tgaaggtgac cagcaaaccg tgaacgatgc cgcacgcatt cgcgctaccg
    18841 gcacaccagc gattcaggtg aacaccggta aaggctgcca tcttgacgca cagatgattg
    18901 ccgacgccgc accgcgtctg ccactggacg ataacggtat tctgtttatc gaaaacgttg
    18961 gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa acacaaagtg gcggtgcttt
    19021 ccgttaccga aggtgaagac aaaccgctga aatatccgca tatgtttgcc gccgcctcgc
    19081 tgatgctgct caacaaagtt gacctgttgc cgtatctcaa ctttgacgtt gagaagtgca
    19141 tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat ccttatttcc gccaccagcg
    19201 gcgaagggat ggaccagtgg ctgaactggc tggagacaca gcgatgtgca taggcgttcc
    19261 cggccagatc cgcaccattg acggtaacca ggcgaaagtc gacgtctgcg gcattcagcg
    19321 cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac ggtcagccgc gcgtgggcca
    19381 gtgggtactg gtacacgttg gctttgccat gagcgtaatt aatgaagccg aagcacgcga
    19441 cactctcgac gccttgcaaa acatgtttga cgttgagccg gatgtcggcg cgctgttgta
    19501 tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg cgccggaaca ggtgatgcag
    19561 ttaattgagc atctgcgcga acgtgcttca catctctctt acaccgccga acgccctctg
    19621 cggattatgg aagtgtgcgg tggtcatacc cacgccattt ttaaattcgg cctcgaccag
    19681 ttactgcctg aaaacgttga gtttatccac ggtccgggtt gcccggtgtg cgtactgccg
    19741 atgggcagaa tcgacacctg cgtggagatt gccagccatc cggcagtcat cttctgtacc
    19801 tttggcgacg ccatgcgcgt gccggggaaa cagggatcgc tgttgcaggc aaaagcacgc
    19861 ggtgccgatg tgcgcatcgt ctattcgccg atggatgcgt tgaaactggc gcaggagaat
    19921 ccaacccgca aagtggtgtt cttcggctta ggttttgaaa ccaccatgcc gaccaccgcc
    19981 atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt tttacttctt ctgccagcat
    20041 attacgctta tcccgacgct gcgcagtttg ctggaacagc cggataacgg tatcgacgcg
    20101 ttcctcgcgc cgggccacgt tagtatggtt atcggcactg atgcctataa ttttatcgcc
    20161 agcgattttc agcgtccgct ggtggtggct ggtttcgaac cccttgatct actgcaaggc
    20221 gtggtcatgc tggtggagca gaaaatagcg gcccacagca aggtagagaa tcagtatcgt
    20281 cgggtggtac cggatgccgg taacctgctg gcgcaacagg cgattgccga tgtgttctgt
    20341 gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg aatcttctgg cgtgcacctg
    20401 acgccggatt atcaacgatt cgatgccgaa gcacatttcc gcccggcacc gcagcaggtc
    20461 tgcgatgacc cgcgcgcgcg ttgtggcgaa gtcttgacgg gcaaatgtaa gccgcatcaa
    20521 tgcccgctgt ttggtaacac ctgtaatcct caaaccgcgt ttggtgcgct gatggtttcc
    20581 tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc aggagagtga agcgtgaata
    20641 atatccaact cgcccacggt agcggcggcc aggcgatgca gcaattaatc aacagcctgt
    20701 ttatggaagc ctttgccaac ccgtggctgg cagagcagga agatcaggca cgtcttgatc
    20761 tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc caccgacagt tacgttattg
    20821 acccgctgtt cttccctggc ggtaatatcg gcaagctggc gatttgcggc accgcgaatg
    20881 acgttgcggt cagtggcgct attccgcgct atctctcctg tggctttatc ctcgaagaag
    20941 gattgccgat ggagacactg aaagccgtag tgaccagcat ggcagaaacc gcccgcacgg
    21001 caggcattgc catcgttact ggcgatacta aagtggtgca gcgcggcgcg gcagataaac
    21061 tgtttatcaa caccgcgggc atgggcgcaa ttccgacgaa tattcactgg ggcgcacaga
    21121 cgctaaccgc aggcgatata ttgctggtta gcggtacact cggcgaccac ggggcgacta
    21181 tccttaacct gcgtgagcag ctggggctgg atggcgaact ggtcagcgac tgcgcggtgc
    21241 tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt gaaagcgctg cgtgatgcca
    21301 cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc agcctgcggt tgcggtattg
    21361 aaatttctga atcagcgctg ccggttaaac ctgccgtgcg cggcgtttgc gaattgctgg
    21421 gactggacgc cctgaacttt gccaacgaag gcaaactggt gatcgccgtt gaacgcaacg
    21481 cggcagagca agtgctggca gcgttacatt cccatccact ggggaaagac gcggcgctga
    21541 ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg tctgtatggc gtgaaacgaa
    21601 ccctcgattt accacacgcc gaaccgcttc cgcgtatatg ctaataaaat tctaaatctc
    21661 ctatagttag tcaatgacct tttgcaccgc tttgcggtgc tttcctggaa gaacaaaatg
    21721 tcatatacac cgatgagtga tctcggacag caagggttgt tcgacatcac tcggacacta
    21781 ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt cgcaactggt aaagcgttct
    21841 gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc agactcaacg tgcgtcttat
    21901 tacgcatcgc gtgaaaaaga cacccccatt aaatatgaag acgaaactgt tctggcacac
    21961 ggtccggtac gcagcatttt gtcgcgccct gatacgttgc attgcagtta cgaagaattt
    22021 tgtgaaacct ggccgcagct ggccgcaggt gggctatacc caaaatttgg tcactattgc
    22081 ctgatgccac tggcggcgga agggcatatt tttggtggct gtgaatttat tcgttatgac
    22141 gatcgcccct ggagcgaaaa agagttcaat cgtctgcaaa catttacgca gatcgtttct
    22201 gtcgtcaccg aacaaatcca gagtcgcgtc gttaacaatg tcgactatga gttgttatgc
    22261 cgggaacgcg ataacttccg catcctggtc gccatcacca acgcggtgct ttcccgcctg
    22321 gatatggacg aactggtcag cgaagtcgcc aaagaaatcc attactattt cgatattgac
    22381 gatatcagta tcgtcttacg cagccaccgt aaaaacaaac tcaacatcta ctccactcac
    22441 tatcttgata aacagcatcc cgcccacgaa cagagcgaag tcgatgaagc cggaaccctc
    22501 accgaacgcg tgttcaaaag taaagagatg ctgctgatta atctccacga gcgggatgat
    22561 ttagccccct atgaacgcat gttgttcgat acctggggca accagattca aaccttgtgc
    22621 ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc tgaaactggc gcaatgtgaa
    22681 gagaaagtgt ttaccactac caatctgaat ttactgcgcc agattgccga acgtgtggca
    22741 atcgctgtcg ataacgccct cgcctatcag gaaatccatc gtctgaaaga acggctggtt
    22801 gatgaaaacc tcgccctgac cgagcagctc aacaatgttg atagtgaatt tggcgagatt
    22861 attggccgca gcgaagccat gtacagcgtg cttaaacaag ttgaaatggt ggcgcaaagt
    22921 gacagtaccg tgctgatcct cggtgaaact ggcacgggta aagagctgat tgcccgtgct
    22981 atccataatc tcagtgggcg taataatcgc cgcatggtca aaatgaactg cgcggcgatg
    23041 cctgccggat tgctggagag cgatctgttt ggtcatgagc gtggggcttt taccggtgcc
    23101 agcgcccagc gtatcggtcg ttttgaactg gcggataaaa gctccctgtt cctcgacgaa
    23161 gtgggcgata tgccactgga gttacagccg aagttgctgc gtgtattgca ggaacaggag
    23221 tttgaacgcc tcggcagcaa caaaatcatt cagacggacg tgcgtctaat cgccgcgact
    23281 aaccgcgatc tgaaaaaaat ggtcgccgac cgtgagttcc gtagcgatct ctattaccgc
    23341 ctgaacgtat tcccgattca cctgccgcca ctacgcgagc gtccggaaga tattccgctg
    23401 ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg ggcgcaatat cgacagcatt
    23461 cctgccgaga cgttgcgcac cttgagcaat atggagtggc cgggtaacgt acgcgaactg
    23521 gaaaacgtca ttgagcgcgc ggtattgcta acacgcggca acgtgctgca gctgtcattg
    23581 ccagatattg ctttaccgga gcctgaaacg ccgcctgccg caacggttgt cgctcaggag
    23641 ggcgaagatg aatatcagtt gattgttcgc gtgctgaaag aaactaacgg cgtggttgcc
    23701 gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca cgaccctgct gtcacggatg
    23761 aagcgactgg gaattgataa atcggcattg atttaactgc aaattgccgg acagatctgc
    23821 ctgtccggca tactattcac gaggtttttt cggacgatat ttttccggca gttctggcac
    23881 cggacacttg tcatcgatga gatgacgcac ggttaagatc ggatgacgcc acagcattct
    23941 cggcccggcc caacgcataa tctgtttcat ctcttcacgc tttgcaggct ggtaacagtg
    24001 caccggacac tgcttacagg ctggtttctc ttcgccgaac acacatttat ccagccgctt
    24061 ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc gctgacgcct gcgggcattt
    24121 cgcttgataa agatcgatca tttttttaat cgtcagtttt tcacgagaga tacgcttgcc
    24181 ggacatactg cctccacctc attaagatgc atttatatta caacttaatc ttaaagggca
    24241 ctatgactct aaagaagaag ggttagccaa ccgatacaat tttgcgtact tgcttcataa
    24301 gcatcacgca aaagctgcaa aacagcatct ttcccggaac cagcatcaag aactcgccgt
    24361 tcgcttcttc ccctgaaatg attaactccg gtatcatgtg cgccttatgt gattacaacg
    24421 aaaataaaaa ccatcacacc ccatttaata tcagggaacc ggacataacc ccatgagtgc
    24481 aatagaaaat ttcgacgccc atacgcccat gatgcagcag tatctcaagc tgaaagccca
    24541 gcatcccgag atcctgctgt tttaccggat gggtgatttt tatgaactgt tttatgacga
    24601 cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc aaacgcggtg cttcggcggg
    24661 agagccgatc ccgatggcgg ggattcccta ccatgcggtg gaaaactacc tcgccaaact
    24721 ggtgaatcag ggcgagtccg ttgccatctg cgaacaaatt ggcgatccgg cgaccagcaa
    24781 aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca ggcaccatca gcgatgaagc
    24841 cctgttgcag gagcgtcagg acaacctgct ggcggctatc tggcaggaca gcaaaggttt
    24901 cggctacgcg acgctggata tcagttccgg tcgttttcgc ctgagcgaac cggctgaccg
    24961 ggaaacgatg gcggcagaac tgcaacgcac taatcctgcg gaactgctgt atgcagaaga
    25021 ttttgctgaa atgtcgttaa ttgaaggccg tcgcggcctg cgccgtcgcc cgctgtggga
    25081 gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa tttgggaccc gcgatctggt
    25141 cggttttggc gtcgagaacg cgccgcgcgg actttgtgct gccggttgtc tgttgcagta
    25201 tgcgaaagat acccaacgta cgactctgcc gcatattcgt tccatcacca tggaacgtga
    25261 gcaggacagc atcattatgg atgccgcgac gcgtcgtaat ctggaaatca cccagaacct
    25321 ggcgggtggt gcggaaaata cgctggcttc tgtgctcgac tgcaccgtca cgccgatggg
    25381 cagccgtatg ctgaaacgct ggctgcatat gccagtgcgc gatacccgcg tgttgcttga
    25441 gcgccagcaa actattggcg cattgcagga tttcaccgcc gagttgcagc cggtactacg
    25501 tcaggtcggc gacctggaac gtattctggc gcgtctggcg ttgcgtaccg ctcgcccacg
    25561 cgatctggcc cgtatgcgtc acgctttcca gcaactgccg gagctgcgtg cgcagttaga
    25621 aactgttgat agtgcaccag tacaggcgct acgtgagaag atgggcgagt ttgccgagct
    25681 gcgcgatctg ctggagcgag caatcatcga cacaccgccg gtgctggtac gcgacggtgg
    25741 tgttatcgca tcaggctata acgaagagct ggatgagtgg cgcgcgctgg ctgacggcgc
    25801 gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa cgtaccggcc tggacacgct
    25861 aaaagttggc tttaatgcgg tgcacggcta ctacattcaa atcagccgtg ggcaaagcca
    25921 tctggcacct atcaactata tgcgtcgcca gacgctgaaa aacgccgagc gctacatcat
    25981 tccagagcta aaagagtacg aagataaagt cctcacttca aaaggcaaag cactggctct
    26041 ggaaaaacag ctttatgaag agctgttcga cctgctgttg ccgcatctgg aagcgttgca
    26101 acagagcgcg agcgcgctgg cggaactcga cgtgctggtg aacctggcgg aacgggccta
    26161 taccctgaac tacacctgcc cgaccttcat tgataaaccg ggcattcgca ttaccgaagg
    26221 ccgccatccg gtggttgaac aggtgctgaa cgagccattt atcgccaacc cgctgaatct
    26281 gtcaccgcag cgccggatgt tgattattac cggtccgaac atgggcggta aaagtaccta
    26341 tatgcgccag accgcactga ttgcgctgat ggcctatatc ggcagctacg taccggcgca
    26401 aaaagtcgag attggcccga ttgaccgtat ctttacccgc gtaggggcag cggatgatct
    26461 ggcttccggg cgttcaacct ttatggtgga gatgaccgaa accgctaata ttctgcataa
    26521 cgccaccgag tacagtctgg tgctgatgga tgagattggg cgcggaacgt ccacttacga
    26581 tggtctgtcg ctggcgtggg cgtgcgcgga aaatctggcg aataagatta aggcgttgac
    26641 gctgtttgcc acccactatt tcgagctgac ccagttaccg gagaaaatgg aaggcgtcgc
    26701 caacgtgcat ctcgatgcac tggagcacgg cgacaccatt gcctttatgc atagcgtgca
    26761 ggatggcgcg gcgagcaaaa gctacggcct ggcggttgca gctctggccg gcgtgccaaa
    26821 agaggttatt aagcgcgcac ggcaaaaact gcgtgagctg gaaagcattt cgccgaacgc
    26881 cgccgctacg caagtggatg gtacgcaaat gtctttgctg tccgtaccgg aagaaacctc
    26941 gcctgcagtc gaggcactgg aaaacctcga tccggattca ctcaccccgc gtcaggcgct
    27001 ggaatggatt tatcgcctga agagtctggt gtaataataa ttcccgatag tcttttgcta
    27061 tcgggaatat taacgataac tgacgaatca aataaaaata ccctgtataa taggaaagct
    27121 tattttacag ggtaaaacca tgccatctac acgctatcaa aaaatcaata cccatcacta
    27181 tcgccatata tgggtcgttg gtgatattca tggtgaatat cagttattac aatcccgctt
    27241 acatcaactc tcttttttcc ccgaaaccga cttacttatt tctgtcggcg ataatattga
    27301 tcgtgggccg gagagtctta acgtcctgcg cctgctaaac cagccctggt ttatctccgt
    27361 taaaggcaac cacgaagcaa tggcgctgga tgcgttcgcg accggcgatg gcaatatgtg
    27421 gcttgccagc ggtggtgact ggtttttcga tttaaatgat tcagagcaaa aagaagctac
    27481 agatctgttg ctgaaattcc atcaccttcc acatattatt gaaatcacta acgacaacat
    27541 aaaatatgtc atcgcacatg cagattatcc ggggagtgaa tatctctttg gtaaagaaat
    27601 agcggagagc gaattactct ggcctgttga tcgtgtgcag aaatcgctta atggcgagtt
    27661 acaacaaata aacggcgctg attattttat atttggacat atgatgtttg ataacattca
    27721 gacgttcgct aaccagattt atattgatac cggatcgccg aaaagcgggc ggctgtcatt
    27781 ttataaaata aagtaatgag gccgggtaaa gcaaagccac tacccggcac tttttattag
    27841 cgcttacctt ccgccagcag cggcgggatg ctcggcacca ttggcgcgtc atcaatatct
    27901 ttctgcgtca tgcggaacgc ttcgggataa tgttcgcggc tggtacggcg cagcggttcg
    27961 gtatcgcgcc aggtataaag gcaatgctgg cactgatata ccgtccagac atctttcacc
    28021 ggcgatttcg ccatcacttc aatctgttca tcggcacaac gtggacaaat catcttgttc
    28081 tccttattta cgtgcggcca gcatagcggt cagtttttca gcccaggctt tggtttccgg
    28141 caaatccacc accggctggc tgtagtgacc acggttgtcc ggggcgacag gcgtggtggc
    28201 gtcgataatc agcttgtcgg tgatccccgc cgggcttgag ccagggtcga gttccagcac
    28261 ggacatattc ggcaactgca ccaaatcccc tgccggattt actttcgagg agagcgccca
    28321 catcacctgc ggcaggttga acgggtcaac gtcttcatcg accataatca ccatctttac
    28381 gtagcccaga ccgtgcggcg tggtcatcgc acgcaggccc accgcgcggg caaagccgcc
    28441 gtagcgtttt ttggtggaga taatcgccag caggccgtgg gtgtacatgg cgtttaccgc
    28501 ctgcacttcc gggaactcgg ctttcagttg ttgatacagc ggcacacagg tggctggccc
    28561 catcaggtag tcgatttcgg tccacggcat gccgaggtac agcgattcga aaatcggcct
    28621 ggtgcggtaa gagactttat cgatacgcac cacggtcatg ttacgcccgc cggagtagtg
    28681 cccggtaaac tcaccgaacg gcccttcgat ttcgcgtttg cggctttcga taaccccttc
    28741 gaggatcact tctgaacccc acggcacatc aaaaccagtc aatggcgcgg tggcgatcgg
    28801 gtacgggctt tcgcgcagcg cgcctgccat ttcatactca gactgatcgt atttcagcgg
    28861 cgtggcgccc ataagggtga tgatcggatc gttacccagg gtgatggcaa tcggcagatc
    28921 ttcaccgcgc tcttccgctt tatgcagatg cagggcgata tcgtgcatcg gcaccggttg
    28981 caggccgagc ttacgcttgc ccttcacttc catgcggtag ataccgacgt tctgcttgcc
    29041 gaagttatcc gggtcgagcg gatcgcggga aaccacgcac gctttgtcga gatagaaacc
    29101 gccatcaccg tcgtttaaac gaaacagcgg caggatatcg aacagattaa tctcgtcacc
    29161 atcgacggtg ttctgcgccc aggctggatt ggcgcggcgc tccggggcga tcgggaagtt
    29221 atcccagcgg cggataaact catcaatctg ttttttaacc ggggtgtttg gcggcaggcc
    29281 caaggaaatc gcgtggttct gccaggaacc gatggtattc atcgccacgc gggcatcggt
    29341 aaaaccgcga atattatcaa accacagcgc cggtgcacca tcgccgatac gcccggtggc
    29401 gttggcagcg gcagccagat ccggttccgc gttcacctct tcgctgattt tcagtaactg
    29461 gccgtggtca tcaagcgcct gtaaaaagct gcgtaaatca tcaaatgcca ttattcattc
    29521 tcctgagaaa aattccgggc ctgcggcaat ccttgccagc gcctggcgta gggatgttca
    29581 aggccaaatt gatccagcac gcgggctacc acgtggtgga caatgtcatc taccgtttcg
    29641 ggatggttat aaaaggcagg catcggcggc accatcgcca cgcccatgcg tgaaagtgcg
    29701 agcatatttt cgagatggat ggtgctaagc ggcatttcac gcggcaccag caccagtttg
    29761 cggccttctt taagcacgac gtccgccgcg cgccctacca ggccatcagc gtaaccagcg
    29821 cggataccgg cgagcgtttt catactgcac ggaataacga tcatgccgtc tgtacgaaag
    29881 gaacctgagg agatggtcgc cgcctgatcc gccgggttat ggctgaagtc agcgagggca
    29941 gcaacatcgc gggcgctgta aggcgtttcc agttcaatgg tggttttcgc ccacttcgac
    30001 atcaccagat gagtctcgac attcggcatc tcccgcagcg cttgcagtaa tgccacacca
    30061 agaggcgcac cggtagcccc tgtcatcccg acgatcagtt tcattgctta ctctcttaat
    30121 tgttcgtata cgaacattat tattaaaata cgcctctgtt ctcattgcgg caagcactga
    30181 aaagtaacaa catccccttc gtcactgaaa aaccgctatg atagcggcag atagtttgcc
    30241 cggagtttac atgacgttac gaaataaagc gttccatcag ttacgacagc tttttcagca
    30301 gcacacggct cgctggcagc acgagttacc tgacctgacc aaaccacagt atgcggtgat
    30361 gcgcgccatt gctgataagc ctggtatcga acaggtggca ctgatagaag cagcagtcag
    30421 caccaaagca acgttggcag aaatgttggc aagaatggag aatcgtggct tagtcagacg
    30481 tgaacatgat gccgctgaca agcggcgtcg ctttgtctgg ctgactgccg aaggagaaaa
    30541 gatacttgct gcggcgatac cgattggtga cagcgtggat gaggaatttt tggggcgttt
    30601 gagtgcagaa gagcaggagc tgtttgtgca gctggtgcgc aagatgatga acacatagga
    30661 tgcaaattgc cgggtaggac gctgacgtgt cttatccagg cgacaaaaac acaagcttcc
    30721 caaacaaaag aaaaaggcca gcctcgcttg agactggcct ttctgacaga tgcttactta
    30781 ctcgcggaac agcgcttcga tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg
    30841 caggccttca acctgaatct ggcgaacacg ttcacgggtg aggccaattt cacgacctac
    30901 atcttccagt gttgccgctt cgtaccccag caaaccgaat cgacgtgcca gtacttcacg
    30961 ctgtttggcg ttcagctcga acagccattt gacgatgctc tgcttcatat cgtcatcttg
    31021 cgtggtatct tccggaccgt tctctttttc atcggccagg atgtccagca acgctttttc
    31081 ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg
    31141 gctgacgtca tcaactggct tatccagttg ctctgcgatc tcttccgcac ttggttcatg
    31201 gtccagctta tgggacaact cacgtgctgt tcgcaggtaa acgttcagct cctttacgat
    31261 gtgaatcggc aaacgaatag tacgggtttg gttcataatc gcccgttcaa tcgtctggcg
    31321 aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa acttctctac
    31381 cgcacggatc agccccaggt tgccctcttc gataaggtcc agcaacgcca gaccacgatt
    31441 gccataacgg cgggcaattt ttaccaccag acgcaagtta ctctcgatca tccggcggcg
    31501 agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa
    31561 cagtggtgaa taaccaatct caccaaggta aagctgagtc gcgtccaaca cacgctgtgt
    31621 ggctccctgc gataacagtt cctcttcggc caaatcgtta tcactgggtt cctcttctac
    31681 taaggccttt tcgtcaaaaa cctcaactcc gttctcatca aattccgcat cttcatttaa
    31741 atcatgaact ttcagcgtat tctgactcat aaggtggctc ctacccgtga tcccttgacg
    31801 gaacattcaa gcaaaagcct ggttccgccg atttatcgct gcggcaaata acgcagcggg
    31861 tttacggatt tccccttgta acgaatttca aaatgcaagc gtgttgaact ggttccggtg
    31921 ctacccatgg ttgctatttt ttgccccgcc ttcacttctt gttgttcccg gaccagcatt
    31981 gtgtcgttat gggcgtaggc actcaggtaa tcatcattat gtttgatgat aatcagatta
    32041 ccgtagccgc gcagcgcgtt accggcataa acaacacggc catctgcggt cgcgataatt
    32101 gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc ccccctcaga agcgccaaag
    32161 gtttcgatca ctttgccctc agtcggccag cgccaggtgg agataggcgt actggttgat
    32221 gtactgctga cagtcggctc ggttgtgctt gctgttggta ccgttacagg cgctgtgacc
    32281 gtggtcgcag ttggcttgtt gttcggcaac attttgttag cactctgttc acccgaagac
    32341 tcagaatacg taattgtcgg ttgcgacgca acagcaacgg tggaattttg tgcaggcttg
    32401 atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc agtgattggc
    32461 gtaccggaag cattacccac ctgtaaggtc tgaccgacgt tcagcgcgta tggtgcctga
    32521 atattgttgc gctgagcaag gtcacggaaa tcgttgccag taatccaggc gatatagaaa
    32581 agcgtgtcgc cttttttcac ggtataggta ctgccgctat aactgccttt cggaatgttc
    32641 ccatactgac ggttatagac gatgcgtccg ttttccatct gtaccggctg ctgagctact
    32701 ggctgcactg gctggatttg cggttgttga gtagcctgaa tttgtggctg ctgcaccggc
    32761 tgaatttgcg gttgctgcgc tgtagacgtc gtccccattt tcggcggcgg cgtaatcaac
    32821 ataccagaat tggtatgtgc aggcgcattg ccattaacgg agctgaccgg tgccggtgga
    32881 tttgaagtgt cagaacagcc tgccagccat agcgaaacca gtgacaaagc cgcaatgcgg
    32941 cgaacggtga attttgggct tcccgcgctc atttatcccc caggaaaaat tggttaataa
    33001 ccagtgacat aattaccgtg caaggcacca tactgaacac tggaaaagat gttcacgata
    33061 cgctgacctg cggcaaaata accaggaaaa atccaggtat ttcctcacgt tttaagccag
    33121 ctcacccttc actaaaggga caaagcgcac ggcctccacg gtatcgataa taaattcgcc
    33181 tccccgacga cgcacccgtt tcaaatactg gtgctcctcc cctacgggta agacgagaat
    33241 cccgccttcg tccagctgcg tcattagcgc agttggaatt tccggcggtg ccgccgtaac
    33301 aatgatagcg tcaaacggcg cacgtgcctg ccaaccttgc catccatcgc catgacgggt
    33361 tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc gcctgccact gcaagccttt
    33421 aatccgttca accgagcaaa catgctggac aagatgcgcc aggattgccg tttgatatcc
    33481 cgaaccggtg ccaatttcca gcacccgcga ctgcggcgtc agctcgagta attcggtcat
    33541 tcgcgccacc atatacggct gcgaaatcgt ctgcccctga cctatcggca aggcgatatt
    33601 gtcccaggct ttttgttcaa acgcttcatc aacgaatttt tcacgcggca cggcggcaag
    33661 tgcattcagc acctgctcat cctgaatacc ttgcgcacgt aattgatcca gaagtgcttg
    33721 tacgcgtctg cttaccattg cgtgccaact cccacgctgt ttaaccagtc tgaaaccaca
    33781 tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg tgatggagac atagccctca
    33841 tctaccgcag caaaatcggt ccccggaccg gcatcacatt taccgcccgg cgggccaatc
    33901 cagtacagcg tattgccgcg cggatcttgc tgcgggatca cctgatctgc cggatgtcgt
    33961 gtaccgcagc gcgtcacgcg aatacctttg atttgatcca agggtaaatc cggaacatta
    34021 atattaagaa tacgcccggt gcgcagcggc tctttacaca gtgcgcgcaa aattgaacag
    34081 gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc aagcgccgga
    34141 aaacctaaat gacggccttc catcgcggcg gctaccgtac cggaataaat aacatcatcc
    34201 cccagattcg gcccggcgtt aattccggac acaacaatgt ccgggcgcgg acgcatcaga
    34261 gcattcacgc caagatagac gcaatcggtc ggggttccca tttgcacagc aatatcacca
    34321 ttttcaaagg taaacgtgcg cagggaggat tccagtgtca gagaatttga agcgccgctg
    34381 cggttacgat cgggggcgac cacctgaacg tcagcaaact cacgcaaggc tttcgccagc
    34441 gtttgtatac cgggtgcatg taccccgtca tcattactca gcaatatgcg cataatcacc
    34501 tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa ctacccgccg gaagccagaa
    34561 acggatttct acggtgacgt catcccacca attccagctt aattgttgcg gatacagcag
    34621 catcgctctg cgcgcggctt caactttttc gcgcaccagt aaagcttgta attcagtttc
    34681 tgcggcgaca gctgcctgtt cgaatgccag cgcttcacgc tgagttcccc attcaccact
    34741 gcccggcaat gcggcggtta tcatcagctc tttatcgttg acgcgacgct gtaattccac
    34801 cagttcttcg gtggttgcaa caaaccagct accacgtccg gctaattgta gcgcatcgcc
    34861 gtcaacaact tgattaacgt ctgctttttt gaggcgctca gcaacaatct gattaaacaa
    34921 cgcactgcgg gctgccgaca accaaaaact ccgtttattg cgatcgcgca ccggagtatt
    34981 ggtttgcgcc cagcgcagcg cgccctgcaa gttgctaccg ccaatcccaa aacgttgggc
    35041 accgaagtag ttcggtacac cttttacgca aatatcgatc agacgttgtt caacgtcatc
    35101 gcgattgctc acttcgcgca gaaccagggt aaaggcgtta cctttcagcg cccctaaacg
    35161 cagcttgcgc ttgtgccgcg catactccag cacctggcag ccttccaatt gaaaggcgct
    35221 cagatcgggc atttccttgc ccggcacgcg agcgcataac cactgttctg taacagcatg
    35281 tttgtctttt tgcccagcaa agctgacttc acgggcatga attttcagga atttcgccag
    35341 tgcatccgcc acaaaacggg tattgcagcc gtttttgagg attctaacca gaatatgctc
    35401 accttcacca tcaggctcaa agcccaaatc ttccaccacc acaaagtctt ccggattggc
    35461 tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa gtgagattat caaactcaat
    35521 cattttgttg ccttaatgag tagcgccacc gcttcacagg caatcccttc cccacgtccg
    35581 gtaaatccaa gtttttccgt agtagtggct ttcacgttaa catcatccat atggcagccg
    35641 agatcttcgg caataaacac gcgcatttgt ggaatgtgcg gcaacatctt cggtgcctga
    35701 gcgatgatag tgacatcgac gttgccaagg gtataaccct tcgcctgaat acgacgccag
    35761 gcttcgcgta gcagctcgcg gctatcggca cctttaaatg ccggatcggt atccgggaac
    35821 agtttgccga tatcccccag cgccgccgcg ccaagcaatg catcggtcaa cgcatggagc
    35881 gccacgtcgc catcagaatg cgccagcaat cctttttcgt aaggaatgcg tacgccacca
    35941 atgataattg ggccttcacc gccaaaggcg tgtacgtcaa aaccgtgtcc aattcgcatt
    36001 atgtattctc ctgatggatg gttcgggtga ggtaaaactc ggccagtgcc aaatcctccg
    36061 ggcgcgtgac tttaatgtta tccgcacggc cttcgaccaa ctgaggatgg aatccgcaat
    36121 attccagcgc cgaggcttcg tcggtaatag ccgcgccttc atttagagcg cgcgtcagac
    36181 agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat aagccgttgc
    36241 gatcaacggt atgagcaatg gcatttttgc ccggttcggc acgtttcata gtatcgcgca
    36301 ctggtgcggc taggatccct cccgtgcggc tggtttcgct caacgccaac aatcgcgcga
    36361 ggtcatcctg atgcagacaa ggacgagcgg cgtcatgcac caatacccac tgcgcgtcgc
    36421 cagcggcttt caaacctgcc agcacggaat cggcacgctc atcaccgcca tctacaacgg
    36481 tgatttgcgg atgattcgcc agaggaagtt gtgcaaaacg gctatcgcca ggacttatgg
    36541 caatgacgac acgtttcacc cggggatgcg ccagcagcgc atacaccgag tgttcaagaa
    36601 tggtttgatt accgattgag agatattgct taggacattc cgtttgcatt cgacggccaa
    36661 atccggccgc cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg ttaattcccg
    36721 ggctgattta tcgattgttt tgccccgcag actgtgcgcg cttcgacgcg tcaggcacca
    36781 gacgataaaa agtttcgccc ggcctggtca tgctgagttc attacgcgca cgctcttcga
    36841 gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa aagttgatcg tttcgcgctt
    36901 taagtttcgc gtttgtagct tgctgtgccg ccacatcatc attgacgcgg gtatagtcat
    36961 gtataccgtt cttaccgaac cacagcgaat actgtagcca gaccagaata gccagcaaca
    37021 gcagcgttag tttacccatc ctgccccctg aaaaacggca tcatcatccc atgcatccga
    37081 agacgactct acatcctctg ttggggatac cgcgacaacg cgggcaaatg taccacattt
    37141 gtccattgtt acgtataccc agggcgtgca gaacataatc tcattattag ttacggtttg
    37201 aattatgaac agaggagaca agaaagtaca aattagccca gtagccacat aaacagtgcg
    37261 ccaaacataa tgcctactgt catcagggtg aaaacaatac tgtagcgtag ctttccgtcc
    37321 atcaatgaat gcagcgcaat ccccaccact accgcaacgg gcatcagcgc cagaaagaaa
    37381 ggccaggtgt agataaagaa gaacagcgtg ttagagccat aaatcaacat cggcatcgcc
    37441 agcgcaaata accaggagat aaaaccgacc acggcaccag gcagtgacca tgtggtttct
    37501 tcatcctcag taaggctgtc gttatttgtt agtgtaatgt tatggctatt acgcatattt
    37561 gatcctgtta ctttgacgaa ccgggcatgg aaacccggtg gtgtctcagg atctgataat
    37621 atcgttctgt ctcaacagat ctaataattg ctgtaccaaa tttgttacta attgttcacc
    37681 attgagatga atttctgccg attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt
    37741 gcgcagttca ccggcacgcg ctttcttata taagcctttt ggatcgcggg cttcgcaaat
    37801 cgccagcggc gtatcgacaa acacttcgat aaagcgccct tctcctacgc gttcgcgaac
    37861 catctggcgt tcggcgcggt gtggcgagat aaatgcggtc agcaccacca gtccggcttc
    37921 aaccatcaaa ttcgccactt caccgacgcg acggatattc tctttacgat cggcatcgct
    37981 aaaaccgaga tcgctgcata atccgtggcg aacattgtcg ccatccagca gatacgtact
    38041 gacgccgagt ttatgtaacg cctcctccag cgccccggcg accgttgatt taccggaccc
    38101 ggagaggccg gtaaaccaca gcactacacc acgatgaccg tggtgtagct cgcgttgttg
    38161 cacagtgacc ggatggctat gccagacgac gttttcgtca tgcagcgcca ttatttctcc
    38221 cccagcaaat cgcgcgcgcc ccagtgtgga aagtggcggc gaaccagggc attcaattcc
    38281 agttcgaatg cactaaattc agatggcgca gcagttgcct ggctaactgg ctcatgcacc
    38341 ataccggcac ctacggtcac attgctcagg cgatcgataa aaatcagccc accggtaacc
    38401 gggttttgct gataacgatc taacaccagt ggctcgtcaa aagtgagatc caccaggccg
    38461 atgccgttca gcggcaggtt ttcaacttcg cgttgggtaa ggttattaat atcaacctga
    38521 taacgaatgc catcaacacg agaacgcgtc ttcttaccgg caattttgat gtcgtaactc
    38581 tggcccgggg aaagcggctg ttccgccatc cataccacat ccaccgacgc gctctgcaca
    38641 gctggtaacg cttcgtctgc cgccagcagc agatcgccac ggctgatgtc gatctcatcc
    38701 gtcagcacca gggtgatagc ttctccggca aaggcttctt cgcgatcacc atcaaaagtc
    38761 acgatccgcg cgacgtttga ttccacacca gagggcagca cttttacacg ttgcccgact
    38821 tccacgcgac cggatgccag cgttccggcg tagccacgaa aatcgagatt tgggcggtta
    38881 acgtactgca ccgggaagcg cattggctgg gcatccacca ctcgctgaat ctccacggtt
    38941 tccagcactt cgagcagtgt cggaccgctg taccacggca tactttcact ttgcgaagcc
    39001 acgttgtcac cttccagtgc ggagagcggc acaaagcgga tatccagatt acccggcagc
    39061 tgcccggcaa aggtcagata atcttcacga atacgggtga acgtctcttc actgtaatcc
    39121 accagatcca ttttgttgat cgccacgacc agatgtttga tccccaacag tgtggagata
    39181 aaactgtgac gacgggtttg atcgagcacg cctttacggg catcgatcag taagatcgcc
    39241 agttcacatg tcgatgcgcc agtcgccata ttgcgggtgt actgctcgtg ccctggagtg
    39301 tcggcgataa taaatttacg cttctcggta gagaaatagc ggtaggccac gtcaatggtg
    39361 atgccctgtt cacgctcagc ttgcaggccg tccaccagca gagccagatc cagcttttcg
    39421 ccctgggtgc cgtgacgctt actgtcatta tgcagcgatg agagctgatc ttcatagatt
    39481 tggcgggtat cgtgcaacag acgaccaatc agggtacttt tgccgtcatc gacgctacca
    39541 caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg
    39601 ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt
    39661 tttcagctcc atagaaccag cttggtcgcg gtcaatcacg cgcccctgac gttcactggt
    39721 ggtggaaacc agcatctctt cgatgatctc cggcagcgtt tgtgcatttg actccaccgc
    39781 accggtcagc ggccagcagc ccagcgtacg gaaacgcacc atccgttttt taatcacttc
    39841 gcccggttgc aggtcgatac ggttgtcatc aatcatcatc aacataccgt cgcgttccag
    39901 aaccggacgt tccgcagcga gatacagcgg cacaatgtcg atattttcca gccagatgta
    39961 ttgccagata tcctgctcgg tccagttaga gagcgggaaa acgcggatgc tttcgccttt
    40021 gttaatctgc ccgttatagt tgtgccacag ctccgggcgc tgatttttcg ggtcccagcg
    40081 atggaagcga tcacggaaag agtagatacg ctctttagca cgggatttct cttcgtcgcg
    40141 gcgcgcacca ccgaaggcgg catcaaaacc gtatttattc agcgcctgct tcaggccttc
    40201 ggtcttcata atatcggtat gtttcgcgct gccgtgcacg aatggattaa tccccatcgc
    40261 cacgccttcc gggttttgat gcaccagcag ctcgcagccg taggctttcg ccgtacgatc
    40321 gcggaactca tacatctcac ggaatttcca gccggtatcg acatgcagca acgggaaagg
    40381 cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg aatctttacc
    40441 gatagagtag agcatcaccg gatttgagaa ttctgccgcc acctcgcgaa taatgtggat
    40501 gctttccgcc tccagttgcc gcaggtgagt aagtcgtatt tgatccataa ccgttccttt
    40561 gcaataccgc tattttcttg ccatcagatg tttcgactat agggagcgta agagaacgaa
    40621 tgaaattacc aattagaatg agtagttcct taacggaata acgatttggc aaagctaata
    40681 tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga agatttgaaa ttgttttagc
    40741 gcagcggcag tttcatacta tggcggtaaa aaaatttgca tggtatttaa ggactcacta
    40801 tgttttccgc attgcgccac cgtaccgctg ccctggcgct cggcgtatgc tttattctcc
    40861 ccgtacacgc ctcgtcacct aaacctggcg attttgccaa tacacaggcg cgacatattg
    40921 ccactttctt tccgggacga atgaccggaa cacccgcaga aatgttatct gccgattata
    40981 ttcggcaaca gtttcagcaa atgggttacc gcagtgatat tcgtacgttt aatagccgat
    41041 atatttatac cgcccgcgat aaccgcaaaa actggcacaa cgtgacggga agtacggtga
    41101 ttgccgctca tgaaggcaaa gcgccgcagc agatcattat tatggcgcat ctggatacct
    41161 atgccccgca gagcgacgca gatgcagatg ccaatctcgg cgggctgacg ttacaaggaa
    41221 tggatgataa cgccgcaggt ttaggtgtca tgctggaact ggcagaacgc ctgaaaaata
    41281 cgcctaccga gtatggtatt cgatttgtgg cgaccagtgg agaagaggaa gggaaattag
    41341 gcgctgagaa tttactcaag cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga
    41401 ttaatctcga taacttaatt gttggcgata aattgtattt caacagcggt gtaaaaaccc
    41461 ctgaagcagt aaggaaatta acgcgcgaca gggcgctggc aattgcgcgt agtcatggaa
    41521 ttgccgcaac gaccaatccg ggtttgaata aaaattatcc gaaaggcact ggatgttgta
    41581 atgacgcaga aatattcgac aaagcgggca ttgctgtact ttcggtggaa gcgacaaact
    41641 ggaatcttgg gaataaagat ggttatcagc aacgcgcaaa aacagccgca ttccctgcgg
    41701 gaaatagctg gcatgacgta agactggata atcagcaaca tattgataaa gcacttcctg
    41761 gaagaataga acgtcgctgc cgtgacgtta tgcggataat gctaccgctg gtgaaggagt
    41821 tggcgaaggc gtcttgatgg gttggaaaat gggagctggg tgttctaccg caggggcggg
    41881 gaattctaag tgatatccat catcgcatcc agtgcgcccg gtttatcccc gctgatgcgg
    41941 ggaacacagc ggcacgctgg attgaacaaa tccctgggcc ggtttatccc cgctggcgcg
    42001 gggaacacaa accgaaacac acgatcaatc cgaatatgag cggtttatcc ccgctggcgc
    42061 ggggaacact ttggtgacag tttttgtcac tgttttggtg acggtttatc cccgctggcg
    42121 cggggaacac actaagcata catatctgtt tttaaacaaa tttattccac atcaacaatc
    42181 taccaactaa attcaaacat ttccttattt ttaaagaaca cataacctat tgattatcaa
    42241 caggaagaaa agaaaccaaa cgtaacccat ccaaatccac cggaatacgt ctgttttctc
    42301 cccaggtctg aaattcaaaa cccgactcgg tattggtcgc ccaggccatc accacatttc
    42361 cgcaaccagc cagttgggta atttgctgcc agatcatctc ccgaatacgt tttgatgtat
    42421 caccaacata cacaccggca cgcacttcca gtagccagat tgcgagccgt ccacgtaagc
    42481 gcggagggac attttctgta acaaccacga ccatgctcat ccgccgcgcc cccggtgacc
    42541 actatcaccc agcgtttcag gttcagggat ggcaggcggt aacatatccg gcgcgggttg
    42601 tggtggttca atttcacctg cagcaaggac tttctcaatt aacggtatta atttgcccgt
    42661 taacttagtg ctacggaaaa tatcgcgaca ggctaatctg acttctttat caggttctgc
    42721 gggttgcctc gctgctattt caaatgcctt tggcacaacc gaatcaaatt taatgatatc
    42781 ggctatgtca taaacaaatg aaagcggttt gccactatga ataaatccaa tagcgggcgc
    42841 atatcccgcg gctaatactg ccgcttcaga aataccgtac agacatgatg tggcagcact
    42901 gatgcagcga ttcacaacat cgcctttttc ccagtcttta ggatcgtatt tgcgaccatt
    42961 ccatttcaca ccatattgtt tcgccagtaa tgcataggtc tggcgaacgc gggatccctc
    43021 aattccccgt agctgatcca ctgaacggcg agctggcggt ggctcacgaa aacgtaattc
    43081 atacattttg cgcaccacct tcaggcgtag atcttccgtt aaagccagct ttgcctggta
    43141 gagtaattta tctgcccgcg cccctccggg ttgtccggaa gagtaaacgc gaacgcccgc
    43201 ttcaccgacc cagaccagca gtgttcccac cgtggcggcc agatgcaccg ccgcgtggga
    43261 aactctcgtt cccggttcga gcataatgca ggcgaccgat cccaccggaa tgtgcgtgcg
    43321 gatcccggtt ttgtcgatca gcacgaaagc gccgtccagt acgtcgattt gaccgtactg
    43381 gaggaagatc atagaggtgc gatcttttaa cgggatcgga ctcagtggta caaacgtcac
    43441 acctctgctc cgggtttgat cagcatcaga ccacagccga acgcgcggct tttgccatac
    43501 ccctgactga gacgttgcag aaataacccg ggatcggtga ccgtaagcat ccccgtataa
    43561 tccacgctac tgaactggat cagttgccgg gagttttccc gccgcagttg ctgttgtcgg
    43621 taggcatcaa ccgaggtatc cagcagtgta aaaccgcttc tctccccctg cgctgccagc
    43681 cagtccagcg ccgcctgttg ttgatgcaac cagacatcac ttccttccgc ctgccccctc
    43741 acctgccgtt tcgcctccat cagcagatcg tggcgcttgc ccgctttaca gattgttgga
    43801 ttcgcccgca ggttgaaaca aagttgttgt ccggtacgca gctcgggcac aaatgaccgg
    43861 cattcgatgg tgaacgtttc actttccgcc gggcgttcct gcgacagtac aaaaaagcga
    43921 aacgcgccct ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaag
    43981 agatcccaca gccactgatg catcacatat tccccgcgat ccaccaaatg cagcaactgc
    44041 gcaggcgaaa gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc
    44101 tgagccacgg cccctgattg atggtgcgtt ccccaaacag ccactgctga cgatttaaag
    44161 gaacatcccg gcgacgtaat attttgctcg cgaccaggcc gtcgtgttcc ccttcccacc
    44221 agcattcatc ctgaagtttc gggagtgaga ctttcagttc gcggaaacta tcctgatact
    44281 gttggtatgc gttacgtaag acatcagacg cgttgccttc gagcagtaac ggcgcaagtg
    44341 gtaacgccag cggatgactt tttcgcccca gataaagcgg aaaaaccgga tgacgtaaac
    44401 cgtcctgcaa ctgttcaagg ctgtaaggcg catcgggggt tgttgccacc gccaccatcc
    44461 accaggcatc ggtgtagtag tcgcgccggg agataatcgc gctcagaaga tcaggggcgc
    44521 tcaactcttc gcgacggctg aaataacgcg ctttacgcac ctcttttggc atctggaccg
    44581 tgtgataatc ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc
    44641 ggttaaacgc gtttaatcgt tcggtatcat cacgccgaat ccctaccccg gcagccagca
    44701 gccccagcaa tgctgagcgc gaaggcagtt catgggtatg acgcacttcg ccgggggcat
    44761 cgacgcccca ggatgccatt ggcccatgaa gctgaaaaat caaatattgg ctcattagcc
    44821 gccccttacg cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg
    44881 tcaaagcttg cgcattcggt cttctgttca tagaccgtat tcatattttc gcgaagcgtt
    44941 gtaatacgct gcaccgccac atctaactgc cgggtgccat taatgggttc atagaaagcc
    45001 gccgccagag aacgtggttg ttcggtgcct ttttctgcca gcgcccagga ggcgtaggca
    45061 cggctggcaa agctgttctg tttgccggtt ggggagactt taagtgcggc ttccgtaaag
    45121 gcgcgcaagg tctgattagc taacgcttcg tcaccgccga ggttttcgac cagcagatct
    45181 ttatcgatgc agatataggt gtagaacagc gcagaaccga atccggtttc accaagatgc
    45241 ccggcaccgg catcttcaga agcctggcgc aaatcatcaa cggcggtgaa aaaatcatct
    45301 tcgacaatcg tttcactgac accaaatgca tgcgcgacct ggcaggcggc ttcaacatta
    45361 aattcgggtt tattcgccag catacgacca aacatagcga tatctactgc catgcgatct
    45421 ttacgtaaca aagcgagatc ttcctctttt ggcgcgcgct tttcttcggc cagttgatgg
    45481 gccagcgctt ttacggcgtc aaattctgcc gggctgatat ggactaattg ttcagtttcg
    45541 gcgttagtga gcggatcttt tggttttttg tcgtttttag ctttcccaag ataatccgca
    45601 atttttgccg cccattcgat ggcttttttc tcttcgatgc ctttctcaat caggatagtt
    45661 gccgcctcac gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca
    45721 aaaagtgcag aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagtcgcgtt
    45781 gctccaccca ggaccacggt tttcggcgct ccggtgtcat cacggttaag gttggctgca
    45841 gggtaagcgg ttaacaaatg aagctgaata aatgtcgtca tgagatagtc ctttattgat
    45901 tttgttcgtt atcgacgtcg ccagcctggt aatattccag cgcccagcgg atacggataa
    45961 attcggttgg ccgctgatga cggcggtgat gattgagcag atcgtcgctc tcctggcacc
    46021 agcggaagac accctctgcc agagagtcaa gattaacgga accgtttaat aatctgacta
    46081 cgcggcgtaa ctggcgcagt aattcatccg gtgtttttac tgccgacagg cgagtaaaac
    46141 gcccctttga cattaccgcc gccagctgcg cagcaaaagg cagccgttca tcaatggctt
    46201 taacattagc gctaagtgcg gctatcagcg ccagtgcggt aatacgccat tcaggttcat
    46261 cctgccactt tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat
    46321 cattaaccgt cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct
    46381 catgccattt gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc
    46441 ctgctttttc tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt
    46501 cttgcgcgcg gtcatgacgc gttccaaatc aacgggctca taaggattgg tgaatacacg
    46561 ctcgtcaaaa tcctgacgtg cgaataacca aatttccttt tgccatttgc cgagtaattc
    46621 atccgcatcc tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt
    46681 gttccagaag tcgatatcca caaaactgaa atcacccctt gcaccttttg gatcggagaa
    46741 ccatgcttct ttcaatgcac tccgtaacag actcagaatc cgtgaagccg tttgcgcagc
    46801 cagccgcagc ttcggtatct ggccttcttt tttattgagc agcagcggga aatggtgttc
    46861 gtaccaacag cgcgctttca tgttgtcgaa atcataacca aatccccaca ggcccacttt
    46921 tgcctgtttc agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt
    46981 gttttctgac ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg
    47041 ttgtggttta acggagtaaa actcaccgcc ctctttaagt ggtacacggt aaggcgttaa
    47101 gggatgctgc cacatggcat aattcgcacc gtaatttttg gtagtcatca aactcagaag
    47161 cgcgtcactc tgctcaccgc aaatatcgca gttgccgact gtcgtggtat taaaatcaat
    47221 acgaatacgc cgcggcattc cccagtacgc ctggagttta ttgacctgat catcggttac
    47281 caccgcaccg gccagttcgc tggtacgcgt cgggccaagc caggggaaaa ccagatcgtc
    47341 aaattttttg ggtagcggta agtcggcttc atcctgcggc atcacgttga gccacagttt
    47401 gcgccacaag ggggcttgtt gattgccctg atactcctgc aattcaatca gagtcgtcat
    47461 cggcccaccg ccgcgtaaac cggtgcgata gcctttgcca cctgacggcg catttaactg
    47521 tagggagaac agagctaacg cagaacaatg agagcatacg tgttcagtca cgccacgctt
    47581 aataaagtgg tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga
    47641 agcgacctga actttatcgc ccatgagcac ctcgaaatcc tgcataaatg aaggtgaatc
    47701 tgggccaaac tggaaagggt gttctaatga cagcaatgct tcccgtagct tttcagcttc
    47761 cagcccgtct tcccagatat catcccaacg acgataatct tttggcgcga aactgctttg
    47821 tagtaacccc agcaaaaact gccatgccgc cccctggaga tctgcccgcg gcgcagcgat
    47881 atcgacaaca ttttcatccg ccagatcgac tggcgccagc ttgcctgttg ttccgtcttt
    47941 aaaacgaacg ggcaaccacg gggttgtcag aagtgaaaac gagttcatat gtatcctctc
    48001 gtaaaaaaga ccatccctgg tcggttgtcc atcattccat ccttttcggt gtatcaacta
    48061 ttcagagagt cctgtcgcag gtatctcaat caaccttgcc aatcaatccc tccctggccg
    48121 aatacccgca actttcgtca tcagtgacta aaatcacgtt tgccatttcc ggatcttgcc
    48181 gctgttcaat gcaccactgc ctgaacgctt ccccttccag taaagaaaac tcatcccgat
    48241 gttttttcca ccagcttcga cgcactctga caacgctcat ttcccatgcg tgagcaccgg
    48301 tggcataagg cttcaccaca ccggcaatac aggtagccag ccacagggaa acagattcct
    48361 cagccagacg tgtcgacagc ttttccggaa ggtaatcgtt gatattggcg gcatagccag
    48421 gcttgaagtt caggacaaac tttttagcca ttgcgcgatc gcagtaatat ttgcccactt
    48481 gctcctgctc gctgcgggca aatccttccg gcattaccac gtcctcaccg tagactgatt
    48541 caataagaag gcgggctgcg tgtggcattt gaatagcgcc ttgctcacgc agtacacgct
    48601 gcgtcagcca gattcgtcca tgatcgggat agacatatgc actgttacgc atggcactgc
    48661 cgaaccattc gtcaccagga gcgtcgtccc agacgggggc cagaatcagc aattcaggag
    48721 gggaacgctc gtcttttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct
    48781 gtaatcgccc cgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt
    48841 ccaggtcaca atcaacgctc tgctctaaga cctgagtaca aatgaggact tttccggcac
    48901 gctgtgaacc gtcttcttta ccaaagcgtg ccagcgtctc catttcaatt cgctggcgat
    48961 cgctaaaagc aaagcggcta tgaaagagtg aaaggctgga agcgggaatg acgccgcggg
    49021 caagcagctg acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc
    49081 attttccctg acttaccgcc gattcgatac gcgcaatact ctcttgttca ctatgaagcc
    49141 aacccacgct gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat
    49201 cggatttcgt gacatgcgtc agccaggggt aatcatcctt ttcaaggaac ggagcttctt
    49261 gctggccctc tgtgccacgc gcaaaggcgg cgacgagttt gtcgcgctgc tgttgggata
    49321 acgtagcaga aagcaaaatg acgctgtttc cgccacgcgc ctgccgctcg atcagccctt
    49381 caagaatgca cgacatgtaa gcatcacagg catggatctc atcagccagc aggattttgt
    49441 tactcaaccc cagaagccgc agattattat gtttaaacgg catcactgcc atcatcgcct
    49501 gatccagcgt gccgacgcca atttcagcca gtagcgcctt cttgttactg ttggcaaacc
    49561 aggccgcaca tccctgactg aatgtttgtt catccggttc ttctgacccg actaaatcac
    49621 cggaccagag tgattcattg aagcggtcca ttaatgtgcg ggcactgtgt gccagcacca
    49681 agctggggcg ggactctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca
    49741 tggcattggc cgttgccatt gttggcaggc caaaaaacaa accctgtgct ttcctcgcag
    49801 ccatcaacct gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacgtctt
    49861 ccagaataaa taactgtggc cctggctggc tgatatccag atccagtacc ttttgctgta
    49921 atggtgtcgg gtgctcaata aaaggaaaca gcgtattaat tccggtgaaa ggtgcggttt
    49981 ctgcttttgg aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt
    50041 aatctttaat atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc
    50101 agtctgcgag tacgactgtt gcagagatat accaggaaag ttgttttaaa agttcaacgc
    50161 cctcgtcatc atcccagaat gtgggaatct ctatgagcgg aaacagtgcc ttgatttcaa
    50221 ggagaaaatc tcgcgcggca gctttgtctt caggcagaaa attatccagc tcatcaatac
    50281 ggtcaggtgg tcgaccatga tgcccggtag ttatggacat ccacatctct attacacgtg
    50341 taagtttacg agaagagagt gaagatgaag gaagcaactc ctcacattca cttaaataat
    50401 aattccacag ccagtaaccc agcgttgaat gagagatctt ttcgtaattc tttctggaac
    50461 cttccggaat cttgagttca ggggccaggt aaagttgctg aaaagagcgg gcaaattttc
    50521 caatatcgtg ccagcacagc aaccaagcga aaaattgagc cgcctgttcc ttgtcagaaa
    50581 tccctaattg acgaaagtaa tcagccagcc cgaagcaatt tcttttaacc attaaatagc
    50641 ccattgcggc cacatccagc gaatgccagc aaagaaggtg atagccgtcg ccaccctctt
    50701 tctcgccacg tcgggttttt ccccagaaat caaagaaagt cacaatattt ttatcct
//
LOCUS       DAZFKY010000007        50019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFKY010000007 DAZFKY010000000
VERSION     DAZFKY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            159..2300
                     /locus_tag="ACHKCL_000387"
     CDS             159..2300
                     /locus_tag="ACHKCL_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO5761430.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHCVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKCL_000388"
     CDS             complement(2364..4256)
                     /gene="parE"
                     /locus_tag="ACHKCL_000388"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5761431.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKCL_000389"
     CDS             complement(4285..4866)
                     /gene="yqiA"
                     /locus_tag="ACHKCL_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5761432.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKCL_000390"
     CDS             complement(4866..5693)
                     /gene="cpdA"
                     /locus_tag="ACHKCL_000390"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5761433.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5718..6140)
                     /gene="yqiB"
                     /locus_tag="ACHKCL_000391"
     CDS             complement(5718..6140)
                     /gene="yqiB"
                     /locus_tag="ACHKCL_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5761434.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKCL_000392"
     CDS             complement(6141..6770)
                     /gene="nudF"
                     /locus_tag="ACHKCL_000392"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5761435.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKCL_000393"
     CDS             6975..8456
                     /gene="tolC"
                     /locus_tag="ACHKCL_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5761436.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8604..9275
                     /gene="ygiB"
                     /locus_tag="ACHKCL_000394"
     CDS             8604..9275
                     /gene="ygiB"
                     /locus_tag="ACHKCL_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5761437.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9281..10441
                     /gene="ygiC"
                     /locus_tag="ACHKCL_000395"
     CDS             9281..10441
                     /gene="ygiC"
                     /locus_tag="ACHKCL_000395"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5761438.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKCL_000396"
     CDS             complement(10479..11267)
                     /gene="ygiD"
                     /locus_tag="ACHKCL_000396"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5761439.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKCL_000397"
     CDS             11410..12183
                     /gene="zupT"
                     /locus_tag="ACHKCL_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5761440.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKCL_000398"
     CDS             complement(12241..12411)
                     /gene="yqiD"
                     /locus_tag="ACHKCL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5761441.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12673..13326)
                     /gene="ribB"
                     /locus_tag="ACHKCL_000399"
     CDS             complement(12673..13326)
                     /gene="ribB"
                     /locus_tag="ACHKCL_000399"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5761442.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13430..13578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13700..13999
                     /gene="ubiK"
                     /locus_tag="ACHKCL_000400"
     CDS             13700..13999
                     /gene="ubiK"
                     /locus_tag="ACHKCL_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5761443.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14034..14234)
                     /gene="glgS"
                     /locus_tag="ACHKCL_000401"
     CDS             complement(14034..14234)
                     /gene="glgS"
                     /locus_tag="ACHKCL_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5761444.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            14503..15117
                     /gene="yqiJ"
                     /locus_tag="ACHKCL_000402"
     CDS             14503..15117
                     /gene="yqiJ"
                     /locus_tag="ACHKCL_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5761445.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            15159..16820
                     /gene="yqiK"
                     /locus_tag="ACHKCL_000403"
     CDS             15159..16820
                     /gene="yqiK"
                     /locus_tag="ACHKCL_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5761446.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            17059..17118
                     /locus_tag="ACHKCL_000404"
     CDS             17059..17118
                     /locus_tag="ACHKCL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5761447.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            17435..17494
                     /gene="ibsE"
                     /locus_tag="ACHKCL_000405"
     CDS             17435..17494
                     /gene="ibsE"
                     /locus_tag="ACHKCL_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO5761448.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(17614..19047)
                     /gene="hldE"
                     /locus_tag="ACHKCL_000406"
     CDS             complement(17614..19047)
                     /gene="hldE"
                     /locus_tag="ACHKCL_000406"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5761449.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19095..21935)
                     /gene="glnE"
                     /locus_tag="ACHKCL_000407"
     CDS             complement(19095..21935)
                     /gene="glnE"
                     /locus_tag="ACHKCL_000407"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5761450.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(21958..23259)
                     /gene="ygiF"
                     /locus_tag="ACHKCL_000408"
     CDS             complement(21958..23259)
                     /gene="ygiF"
                     /locus_tag="ACHKCL_000408"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5761451.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            23501..24121
                     /gene="ygiM"
                     /locus_tag="ACHKCL_000409"
     CDS             23501..24121
                     /gene="ygiM"
                     /locus_tag="ACHKCL_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5761452.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            24185..25423
                     /gene="cca"
                     /locus_tag="ACHKCL_000410"
     CDS             24185..25423
                     /gene="cca"
                     /locus_tag="ACHKCL_000410"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5761453.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(25604..26425)
                     /gene="bacA"
                     /locus_tag="ACHKCL_000411"
     CDS             complement(25604..26425)
                     /gene="bacA"
                     /locus_tag="ACHKCL_000411"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5761454.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(26516..26884)
                     /gene="folB"
                     /locus_tag="ACHKCL_000412"
     CDS             complement(26516..26884)
                     /gene="folB"
                     /locus_tag="ACHKCL_000412"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5761455.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            26990..27607
                     /gene="plsY"
                     /locus_tag="ACHKCL_000413"
     CDS             26990..27607
                     /gene="plsY"
                     /locus_tag="ACHKCL_000413"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5761456.1"
                     /translation="MSAIAPGMILIAYLSGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(27620..28552)
                     /gene="ttdR"
                     /locus_tag="ACHKCL_000414"
                     /gene_synonym="dan"
     CDS             complement(27620..28552)
                     /gene="ttdR"
                     /locus_tag="ACHKCL_000414"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5761457.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            28759..29670
                     /gene="ttdA"
                     /locus_tag="ACHKCL_000415"
     CDS             28759..29670
                     /gene="ttdA"
                     /locus_tag="ACHKCL_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5761458.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            29667..30272
                     /gene="ttdB"
                     /locus_tag="ACHKCL_000416"
     CDS             29667..30272
                     /gene="ttdB"
                     /locus_tag="ACHKCL_000416"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5761459.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            30321..31784
                     /gene="ttdT"
                     /locus_tag="ACHKCL_000417"
     CDS             30321..31784
                     /gene="ttdT"
                     /locus_tag="ACHKCL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5761460.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(31827..32840)
                     /gene="tsaD"
                     /locus_tag="ACHKCL_000418"
     CDS             complement(31827..32840)
                     /gene="tsaD"
                     /locus_tag="ACHKCL_000418"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5761461.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDLTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            33078..33293
                     /gene="rpsU"
                     /locus_tag="ACHKCL_000419"
     CDS             33078..33293
                     /gene="rpsU"
                     /locus_tag="ACHKCL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5761462.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            33404..35149
                     /gene="dnaG"
                     /locus_tag="ACHKCL_000420"
     CDS             33404..35149
                     /gene="dnaG"
                     /locus_tag="ACHKCL_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5761463.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            35344..37185
                     /gene="rpoD"
                     /locus_tag="ACHKCL_000421"
     CDS             35344..37185
                     /gene="rpoD"
                     /locus_tag="ACHKCL_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5761464.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37264..37770)
                     /gene="mug"
                     /locus_tag="ACHKCL_000422"
     CDS             complement(37264..37770)
                     /gene="mug"
                     /locus_tag="ACHKCL_000422"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5761465.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            37895..37970
                     /locus_tag="ACHKCL_000423"
     tRNA            37895..37970
                     /locus_tag="ACHKCL_000423"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37928..37930,aa:Ile,seq:cat)
     gene            complement(38024..38788)
                     /gene="nfeF"
                     /locus_tag="ACHKCL_000424"
                     /gene_synonym="yqjH"
     CDS             complement(38024..38788)
                     /gene="nfeF"
                     /locus_tag="ACHKCL_000424"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5761466.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            39076..39699
                     /gene="nfeR"
                     /locus_tag="ACHKCL_000425"
                     /gene_synonym="yqjI"
     CDS             39076..39699
                     /gene="nfeR"
                     /locus_tag="ACHKCL_000425"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5761467.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39853..41373)
                     /gene="aer"
                     /locus_tag="ACHKCL_000426"
     CDS             complement(39853..41373)
                     /gene="aer"
                     /locus_tag="ACHKCL_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5761468.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            41791..43170
                     /gene="ygjG"
                     /locus_tag="ACHKCL_000427"
     CDS             41791..43170
                     /gene="ygjG"
                     /locus_tag="ACHKCL_000427"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5761469.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(43212..43544)
                     /gene="ygjH"
                     /locus_tag="ACHKCL_000428"
     CDS             complement(43212..43544)
                     /gene="ygjH"
                     /locus_tag="ACHKCL_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5761470.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            43763..44746
                     /gene="ebgR"
                     /locus_tag="ACHKCL_000429"
     CDS             43763..44746
                     /gene="ebgR"
                     /locus_tag="ACHKCL_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5761471.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44930..48022
                     /gene="ebgA"
                     /locus_tag="ACHKCL_000430"
     CDS             44930..48022
                     /gene="ebgA"
                     /locus_tag="ACHKCL_000430"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5761472.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            48019..48468
                     /gene="ebgC"
                     /locus_tag="ACHKCL_000431"
     CDS             48019..48468
                     /gene="ebgC"
                     /locus_tag="ACHKCL_000431"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5761473.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            48531..49964
                     /gene="ygjI"
                     /locus_tag="ACHKCL_000432"
     CDS             48531..49964
                     /gene="ygjI"
                     /locus_tag="ACHKCL_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5761474.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT        12272 a        13449 c        12554 g        11744 t
ORIGIN      
        1 attttttatt atttctcatt tactactaca ttaccgcatg attgaatgat tctcaattac
       61 atatccgatg gtaaaataac aaacggatag tgcttagtga gcaacaagaa acaattttca
      121 ctcacgacaa tccgtgagtt tttgggataa cagactaaat ggctaagttc acaccttcat
      181 tctcaggaat caaaggtcgg gcgctctttt cactgctctt tgcagcacca atgattcatg
      241 caaccgacac tgcaacgacc aaagatggcg aaacaatcac tgttacagcg gatgcaaata
      301 ccgcaactga ggcgaccgat ggttatcaac ctctgagcac ttccacggcg acattaaccg
      361 atatgccgat gctggatatc ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa
      421 atcagaatgc aacgacgctg gatgaagcgc tttataacgt cagtaacgtg gtacagacca
      481 atacattagg cggaactcag gacgcctttg tacgtcgtgg ttttggtgct aaccgggatg
      541 gctccatcat gaccaacggc ctgcgaactg tacttcctcg cagtttcaac gccgccacag
      601 aacgtgtgga agtgctaaaa ggtccggcct ccacgctgta tggcattctc gatcctggtg
      661 gattgattaa cgtcgtgacc aagcgcccgg aaaaaacatt ccatggttct gtctcagcca
      721 cgtcctccag ttttggcgga ggcactgggc aacttgatat cacaggtccc attgaaggca
      781 ctcagctggc ataccgcctg acgggggaag tgcaggatga agattactgg cgaaacttcg
      841 gtaaagagcg cagtacattt attgccccgt cactcacctg gtttggtgat aatgcaacag
      901 taaccatgct ctattcccat cgggactata aaactccgtt cgatcgtgga acgattttcg
      961 accttacgac gaaacagccc gtaaacgttg atcgaaaaat acgttttgac gaaccgttta
     1021 atattacaga tggtcagtcc gatctggcgc aactcaacgc agaatatcat ctcaatagcc
     1081 agtggacagc gcgctttgat tacagctaca gccaggataa atacagcgac aatcaggctc
     1141 gcgttaccgc gtatgatgca acgacaggaa cgctgacacg gcgtgttgat gcaactcagg
     1201 gatctaccca gcgtatgcat tctactcgtg cggatctgca agggaatgtt gatattgctg
     1261 ggttctataa tgagattctg ggtggggtgt catatgaata ttatgatctt ctgcgcacag
     1321 atatgattcg ctgtaaaaac gctaaagatt tcaatatcta caaccccgtt tatggcaata
     1381 ccagcaaatg cacaacggtt tcggcgtcgg acagtgatca gacgatcaaa caggagagct
     1441 actcagctta tgcacaggat gcgctctatc tgaccgataa ctggattgcc gtcgccggga
     1501 tccgctatca gtattacacg cagtatgcgg gtaaaggccg tccttttaat gtcaatactg
     1561 acagccgcga tgaacaatgg acgcccaaac tggggttagt ctacaaactg acgccatcgg
     1621 tatccttatt tgctaattat tcgcaaacat ttatgccgca atcgtcaatt gccagctaca
     1681 tcggagatct tccaccggaa tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt
     1741 tcgatggtat aaccgcagat attgcgctgt ttgatatcca taaacgtaac gtgttgtata
     1801 ccgaaagtat tggtgatgaa accatcgcca aaacggcagg ccgcgttcgt tcaagagggg
     1861 tagaagtcga ccttgcggga gcattaactg aaaacattaa tatcattgcc agctacggct
     1921 ataccgatgc taaggttctg gaagatcctg attatgcagg gaaaccattg ccgaatgttc
     1981 ctcgtcatac cggttcgcta ttcctgacct atgacattca taacatgcca ggcaataaca
     2041 cactgacgtt tggcggtggt ggacattgcg taagccgtcg ttcggcaacc aatggggctg
     2101 actattatct gccaggctat ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc
     2161 agtatccggt cacactgcaa ttaaacgtca aaaacctgtt tgataaaacg tattacacct
     2221 cttccatcgc cacaaataat ctggggaatc agattggcga tccgcgtgaa gtgcaattca
     2281 cggtgaaaat ggaattttga tcaaaaaagc ccggtataca cttgtatacc gggctatgtt
     2341 ttattcttct ggcgggagga ttatcagacc tcaatctccg ccatgtcgcc tttctcttgc
     2401 aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat catcgcgtca
     2461 gtacgctgat cgtcttcatc atcgatagtc agctgcacca gacggcgagt gttcggatca
     2521 agcgtggttt cgcgcaattg catcgggttc atttccccca gacctttaaa acgctggacg
     2581 ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc ttcttccgtc
     2641 agcgcgtaat aaacctcttt cccgagatca atacggtaga gcggtggcag tgcgacgtaa
     2701 acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga acaaagcgca gagcagtgta
     2761 gcaatgtgca aaccatcaga gtccgcatcc gcgaggatac agattttgcc ataacgaagc
     2821 tggctcagat cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc atgcacttcc
     2881 tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt taaggatctt acctttcagc
     2941 ggcatgatcg cctgatattc gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca
     3001 ccttccacga ggaacagttc ggtacggtta aggtcctgcg cggtacaatc agccagtttg
     3061 ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca cttttttagc cgcacgcata
     3121 cgacgctggg cgctggaaat cgccatctct gccagcaact ccgccgcctg aacgttctgg
     3181 ttcagccaca ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc acactgacgc
     3241 gaagagagac gctctttggt ctgcccggca aactgtggat cctgcatttt tactgacagc
     3301 acataggcgc agcgatccca gatatcttcc gccgacaact ttacaccgcg cggcaaaatg
     3361 ttgcggtatt cacagaactc acgcatcgcg tccagcaggc cctgacgcag gccattaaca
     3421 tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag cagttcaccg
     3481 ccttccggca gccacagtag cgcccagtcc accgcttcag tatcgccagc gaaattaccg
     3541 ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc
     3601 agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc
     3661 tcaacgccgg ggcacagtac tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa
     3721 aaacgcgggc tatcgaagaa ggtttcatcc ggccagaaat ggacgctggt gccggtattg
     3781 cgtttaccgc aattgccgac aacctgtaaa tcctgcacct tttcaccatt ttcaaaggcg
     3841 atgttataaa cctgaccatc gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta
     3901 accaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt gttagagaat
     3961 ttaccgcctg catgcagacg gcaaagaatc agttcaaccg ccggtacacc ctcttccggg
     4021 tgaatatcca ccggcatccc gcgcccatcg tcaataactt ctaacgactg gtcagcatgt
     4081 aaaataacgt ccacgcgttt tgcgtgaccc gccagtgctt catccacact gttatcaatg
     4141 acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg acggcggcga
     4201 accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg cgtcatggtt
     4261 taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg
     4321 gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga cagtctggcg
     4381 gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc acttcatctc ccgtctgttg
     4441 cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt taagatcgta
     4501 aatatggcgt gactctagca catattgctg cccggtgtag gggttctcgt tctgaccgag
     4561 atagtccgtc agcagttcaa acgggcgcac cgccgggttt accaccactg cgggcagcat
     4621 aaaacattgt gacaaccagg tggcgtaata tccccccagt gacgaaccga caatacccag
     4681 cgaatcgccg ccatgttcca ggacaatgga ttccagcagc tctgccgcgt cggaaggata
     4741 cggcggcaac tgcggaatga tcatctcaac gtcagggtga tgttccgcca gccagttttt
     4801 taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt
     4861 agacatcagt agccttctga agcggtatca ggttggaaac gtgtgtccgc caggcgatgc
     4921 acctcggtgg tcagcgtgcc atcagcatgt aactcgagag tacgccagcc gggcgcgata
     4981 gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga cggtgttgcc
     5041 agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc gcacagcaag
     5101 tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg
     5161 tgttgatcaa gccaactaca acccgcaggt agcggatgat gatgcagcag cagcaacgta
     5221 tggcgctctg gcgcatcagc cagtttacgt tccagccact caagctgaaa ctcgcttagc
     5281 tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca ttgctcacca
     5341 ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcgctgta catcgcgggc
     5401 tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc gatgccttca
     5461 gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt cgcgacaatc
     5521 aggtcgaatt cgtgctggtg tggacgaatc gcctccagca ccgcctggta gctttcccag
     5581 gtgtttaccc ctaagagggc ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt
     5641 aaaatcctga ctctggcctc accagccaga ggaagggtta acaggctttc caaatggtgt
     5701 ccttaggttt cacgacgcta ataaaccgga atcgccatcg ctccatgtgc taaacagtat
     5761 cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt
     5821 ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga acacacttca
     5881 gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca gtaactgatc
     5941 gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc
     6001 agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc gtcattgcgc
     6061 ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag
     6121 tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg atgcagctgc
     6181 agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc tacccactgg
     6241 tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc agccagaccg
     6301 tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga acgctcactg
     6361 gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac tatcagcccc
     6421 gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc ttcaatcatt
     6481 ccggcaacca tctccagtag ccaaggggtt tcgctagtgt cgtatgcggc aattcgaatc
     6541 tgctcaatca gcacaacttc atcacgcact gggtcaaagg gtagcaagac tgcggcgtga
     6601 ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa tagacgatga
     6661 cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt
     6721 tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc
     6781 ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt
     6841 catgtgttaa attgaggcac attaacgccc tatggcacgt aacgccaacc ttttgcggta
     6901 gcggcttctg ctagaatccg caataatttt acagtttgat cgcgctaaat actgcttcac
     6961 cacaaggaat gcaaatgaag aaattgctcc ccattcttat cggcctgagc ctttctgggt
     7021 tcagttcgtt gagccaggcc gagaacctga tgcaagttta tcagcaagca cgccttagta
     7081 acccggaatt gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag
     7141 cgcgcagtcc attactgcca cagctaggtt taggtgcaga ttacacctat agcaacggct
     7201 accgcgacgc gaacggcatc aactctaacg cgaccagtgc gtccctgcag ttaactcaat
     7261 ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca gcagggattc
     7321 aggacgtcac gtatcagacc gatcagcaaa ccttgatcct caacaccgcg accgcttatt
     7381 tcaacgtgtt gaatgctatt gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct
     7441 accgtcaatt agatcaaacc acccaacgtt ttaacgtggg cctggtagcg atcaccgacg
     7501 tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa cgaagtgacc gcacgtaata
     7561 accttgataa cgcggtagag cagctgcgcc agatcaccgg taactactat ccggaactgg
     7621 cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac gcgctgctga
     7681 aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag gacctggcgc
     7741 gcgagcaaat tcgccaggcg caggatggtc acttaccgac tctggattta acggcttcta
     7801 gcgggatttc tgacacctct tatagcggtt cgaaaacccg tggtgccgct ggtacccagt
     7861 atgacgatag caatatgggc cagaacaaag ttggcctgag cttctcgctg ccgatttatc
     7921 agggcggaat ggttaactcg caggtgaaac aggcacagta caactttgtt ggtgccagcg
     7981 agcaactgga aagcgcgcat cgtagcgtcg tgcagaccgt acgttcctct ttcaacaaca
     8041 ttaatgcttc tatcagtagt attaacgcct acaaacaagc cgtagtttcc gctcaaagct
     8101 cattagacgc gatggaagcg ggctactcgg tcggtacgcg taccattgtt gatgtgttgg
     8161 atgcgaccac cacgctgtac aacgccaagc aagagctggc gaatgcgcgt tataactacc
     8221 tgattaatca gctgaatatt aagtcagccc tgggtacgtt gaacgagcag gatctgctgg
     8281 cactgaacaa tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa
     8341 cgccggaaca gaatgctatt gctgatggtt atgcgcctga tagcccggca cccgtcgttc
     8401 agcaaacatc cgcacgcact accaccagta acggtcataa ccctttccgt aactgatgac
     8461 gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc
     8521 gcattcttcc cccttctcgc ttcaatttcg accagccatc ctctattctg atgggtattt
     8581 accacaggtc ccggaagaca aaaatgaaac ggacaaaatc catacgccac gcatcgttcc
     8641 gcaaaaactg gagcgcacgc catctgacac cagtcgctct cgcggttgcc actgttttta
     8701 tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat gctgacgact
     8761 gttcagctgc aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga
     8821 aagaagccga acgtactgcg ccgaaatacg ccacccgtga agactgtgtt gctgaatttg
     8881 gtgaaggtca gtgccagcag gcaccagccc aggctggcat ggcaccagaa aaccaggcgc
     8941 aggcccagca atccagcggg agtttctgga tgccgctgat ggccggttac atgatgggtc
     9001 gtctgatggg cggcggtgcg ggatttgcac agcagccgct gttctcctcg aaaaacccag
     9061 ccagcccggc ttacggtaaa tataccgacg cgacgggtaa aaactatggc gcagcccagc
     9121 caggccgcac catgaccgta ccgaagacag ccatggcacc aaaaccggcg accaccacta
     9181 ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg
     9241 caaccggtac ctcttctcgt tcaatgggtg gctgataccg atggaaagag tcagtattac
     9301 cgagcgcccg gactggcgtg agaaagccca cgaatacggt ttcaattttc acaccatgta
     9361 cggcgagccg tactggtgtg aagatgctta ctacaagttg accctcgccc aggttgaaaa
     9421 gctggaagaa gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat
     9481 cgccagcgat gagctgatga ccaaattccg cattccaaaa cacacctgga gttttgtgcg
     9541 ccagtcatgg ctgacgcacc agccatctct ttattcgcgt cttgatctgg cgtgggatgg
     9601 cactggcgaa cctaaacttc tggaaaataa cgccgatacg ccaacgtcac tatacgaggc
     9661 ggcgttcttt cagtggatct ggctggaaga tcagcttaac gccggtaact tgccggaggg
     9721 cagcgaccag tttaacagtc tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga
     9781 acagtatggc ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg
     9841 taccattcag tatttgcagg actgcgcgac ggaagctgaa atcgctaccg agttcctcta
     9901 cattgacgat atcgggttag gtgaaaaagg tcagttcacg gatttacagg atcaggtaat
     9961 ttccaacctg ttcaagctgt atccgtggga atttatgttg cgtgagatgt tttccaccaa
    10021 gctggaggat gcaggcgtac gctggctgga accggcgtgg aagagcatta tctccaacaa
    10081 ggcacttcta ccgctactgt gggagatgtt cccgaatcac ccgaacctgc tgcccgctta
    10141 ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg
    10201 tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc attgaagcag cggaaggtcc
    10261 gtatggcgaa gaagggatga ttgttcagca attccacccg ttaccgaaat tcggcgacag
    10321 ctatatgctg attggtagct ggctggtgaa cgatcaaccc gccggaattg gcattcgtga
    10381 agaccgtgca ttgatcaccc aggatatgtc tcggttttat ccacatattt ttgttgaata
    10441 atcaatgatg ccggatggca ttcgccatcc ggtaattgtt aacctatctg caccgacagc
    10501 atacttaaac tgcccatttc tataccatcg accggaatgg taattggctc ctgcccatcc
    10561 cacgcaccta agacatacag caacggcaga tagtgctctg gcgttgggtt cgataacgcg
    10621 ccaccttcat ggtcgagata attcaccaga ggatgctgct ctaccggccc ttgccacgtc
    10681 agattcgctt tcacatactc attaaacgac atcgcccaag gatacggtga actatcaccg
    10741 tgccacttca ctgtacgcag gttatgcacc acgttgccgc tggcgaccaa cattattcct
    10801 tcatcacgca gcgccgcgag ttttcgcccc atttcgaaat gccaggcggc aggtttgcta
    10861 ctgtcgatac tcaactgcac catcgggata tccgcgtcag gatacatctt aatcagcacg
    10921 ccccaggaac cgtggtcaaa gccccaggct tctttatcca gcgtcaccgg gatcggcgct
    10981 aacagctcaa ccagacgctg tgccagcgca ggcgaacccg gagcaggata atgcgtatcg
    11041 tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc
    11101 actcctgttc cacgggtaaa ccagtgagcc gaaactacca caatcgcttg cgggcgtggc
    11161 aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat tatcttccag cacgttcatc
    11221 ggactaccgt gacctaaaaa caatgctggc atacgtgttg aagacatgat gatatcctta
    11281 actaaaggtg tcattttgat atcctcacga tacgcttgtt cggcggagta agaacccgga
    11341 taacaatgat gatgatcatc agttattttg acgatctgcc tgaaggtgaa gatttataag
    11401 gagttgtcga tgtcagtacc tctcattctg accatactgg cgggggcagc cacgtttatt
    11461 ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta
    11521 ggatttgcgg cggggatcat gttgctcatc tcattaatgg aaatgcttcc tgccgcacta
    11581 gcagctgaag gaatgtcgcc tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc
    11641 tattttggcc tggaccgcat gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg
    11701 gtgcagccgt tgccaaaatc gatcaagcgc acagccattc tgctcactct cggcatcagt
    11761 ctgcataact tcccggaagg gattgccacc tttgtcacgg cgagcagcaa cctggagctg
    11821 ggatttggca tcgcactggc cgtcgcgttg cacaatattc ctgaaggtct ggcggttgca
    11881 ggcccggttt atgcagcaac gggttctaaa cgcacggcaa ttctgtgggc ggggatttct
    11941 ggactggcag aaattcttgg tggtgtgctg gcgtggttaa tcctcggtag catgatttcc
    12001 ccggtggtca tggcagcaat catggcggcg gtcgcaggaa ttatggtggc gctctcggtt
    12061 gatgaattaa tgccgctcgc caaagagatt gaccctaata ataaccccag ctatggcgta
    12121 ctatgtggaa tgtcagtgat gggattcagt ttggtgctgc tacaaacggc gggaattggt
    12181 taaaagcaac atcagaagtt gtccagcgca gggacaactt ctgctagttt cactaaagcc
    12241 ttattctacc ttttcggctg tctcttctct cgtactgttt aagtatttgt tccgcgcttc
    12301 aagcagtgcg tctctgtcct ggcgaaatgc ccgacaataa cgtttcaaat gcaggaaata
    12361 acccaccaca accagaacaa tcaatacaat ccagtaccag gcgataaaca tccttttacc
    12421 cttaatattt aacaatacta aacatttagc gcataaattt cacatatcct ttttcggata
    12481 tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa
    12541 tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagt
    12601 agggaaaaat aaaaaccgaa gcctgctaca acttcggttt cttgattaag gcagtaaatt
    12661 aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc
    12721 aatagtcacg agcgccatgt tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc
    12781 catcgtgcca tcgtcattag tcagctcaca cagcacacca gccggtttga agcctgccag
    12841 cgtcattaga tcaatagttg cttcagtatg accgccacgc gtcagtacac caccagcctg
    12901 agcgcggagt gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc
    12961 aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag tcacaccttc
    13021 agccgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat
    13081 cattggcaga tcgagttgtt tacggcgatc ttcagtaatg cacaggcaaa caataccact
    13141 accgtggcga atggtcagcg ccatctgctc aacagtcatg gtttctgccg ggaagatcat
    13201 atcaccttcg ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag
    13261 cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg
    13321 attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca gtcttaggag
    13381 tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc gttactctct
    13441 cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc ctttgagttc
    13501 gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg ggaatttcgc
    13561 cccgccctga gaataagcgg attcactata acgctaatga ttagcggcag caacgcatag
    13621 cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac tcaacaccta
    13681 tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt
    13741 tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga aaaaaatccg
    13801 ccaaacccta caagcgcagc tgacgcgtct cgatctggta agccgcgaag aattcgacgt
    13861 tcagacgcaa gtcctgttgc ggactcgtga aaaactggcg ttactggagc agcgcagcag
    13921 tgaactggaa gcgcgcaata attcggtagc agatcttcaa tctcctccgg ctatcccgcc
    13981 tattgataaa gcagaataaa gcaacacaaa aacgggccag acggcccgta atatcagtgt
    14041 tctaattcaa gttctctggt ttgcatcatc tgttggcaat accaggcata acgtgcgcaa
    14101 aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc gaccgggcga
    14161 ccttctgcgt gcattctggc aaaactacgc gctaggaaat cgaaattatt taaagaatta
    14221 agactatgat ccataagcac atcctccggt tatcgcatac cttttaaggc catatgcttt
    14281 ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg cgtaaatata
    14341 ttgtccccga tcacactttt tagtgaaaaa taaaaaacca tattaaaaga ctttctcttc
    14401 cagaacctct ctaacatatg ttattattaa caaagacaaa tgtaagcaag aaaactatca
    14461 caaagtaaat attgcgtcac taaatggaca ttggagtgac atatgatttt attcgccgac
    14521 tataacaccc cttatctttt cgctatttcc tttgtactgt tgatcggcct tttagaaatt
    14581 tttgcgctta tctgcggtca catgctctcc ggtgctcttg atgcacatct tgaccattat
    14641 gattccataa ccacaggtca tattagccag gcacttcatt atctcaatat cggaagattg
    14701 ccagctctcg tcgttctttg cttactggct ggtttttttg gtcttattgg catcctgttg
    14761 cagcatacct gcatcatggt ctggcaatca ccactctcga acctgtttgt ggttcctgtc
    14821 agtttactat ttacgattat tgcggtgcat tataccggta aaatagttgc gccctggatt
    14881 ccacgcgatc atagctcagc tattacagaa gaagaatata ttggtagcat ggcgttaatt
    14941 accggtcatc aagccacttc aggtaatccc tgcgaaggaa aactcaccga tcattttggc
    15001 caaatacatt atttactact ggagcctgaa gaaggaaaaa ttttcactaa aggagacaaa
    15061 gtgttaatta tttgccgact ttctgcaacg cgatatttgg cggaaaacaa tccctagccc
    15121 caaatattgt aatttattaa tcaaaaggaa atttaataat ggatgatatt gttaattccg
    15181 tgccctcctg gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta
    15241 ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc atttgtccgt accggcttag
    15301 gggggcaaaa agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa
    15361 tccccatcaa tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga
    15421 ttacgaaaga tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt
    15481 cagtagaagg gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag
    15541 acttacgtat gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa
    15601 tgaccatgca tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg
    15661 tagctgaaga tctgtcgaaa aacggcctgg aactggaaag cgtttcactt accaacttta
    15721 accagacctc gaaagaacat ttcaatccta acaatgcctt tgacgccgaa ggtttaacca
    15781 aactgactca ggagacggag cgccgtcgcc gcgagcgtaa cgaagttgaa caggatgtag
    15841 aagttgcggt gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac
    15901 aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga
    15961 atgcgaaaat tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc
    16021 tggctgaacg acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa
    16081 aagttgaagc tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa
    16141 tcgctaacca gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag
    16201 aagcgcgtgc taaccttgca ctcgcagaag cagtaagcgc ccaacaaaac gtagaaacca
    16261 ctcgccagac agccgaagcc gatcgtgcca agcaggtggc gctgattgcc gcagcacagg
    16321 atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag
    16381 agatgcaggc ggcggcgatc gttgagttag ccgaagctac gcgtaaaaaa ggcctggcgg
    16441 aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgac gaacaaacca
    16501 gccttaaatt caaactggcc ttgttgcagg cgctacctgc ggtaatagag aaatccgtgg
    16561 aaccaatgaa gtccattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggtg
    16621 gtactgcggg ggatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat
    16681 tatcagccgc actctcttac cgcacacagg caccactgat tgactccttg ctcaatgaaa
    16741 ttggcgtttc aggcggctca ctggctgcat tgacttcact cttaacccca acaactcccg
    16801 tcgccgaaaa cgtagaataa attttcagct atgaacatcc ctggctcaaa aagccgggga
    16861 ttttttatat ctgcgttccg ctaaaaggtg caaatgatca ggctgttgca gcgttttgcg
    16921 tgattgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
    16981 tcaacaatcg gggagtaagc aaagggctga aacgggaaag cccctcccga ggaaggggcc
    17041 ttacagaagg aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta
    17101 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc
    17161 ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg
    17221 cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac
    17281 gttgcagcgt tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg
    17341 cggggtccgg cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc
    17401 tcccgaggaa ggggccttat agaaggaaag ggttatgatg aagctcgtca tcatactggt
    17461 tgtgttgtta ctgttaagtt tcccgactta ctaacaactc atcagagggg ggagaaatcc
    17521 tcccttaccc ttgttccttt actctaggtt gaaaagacaa cagcgtcaat aggcctgcca
    17581 tgtacgaagc gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt
    17641 cttgataatg ttagtggtcg agcaaccgtc ttcaaagttg agcaccaaca cttcgccgcc
    17701 gttggcccag acttctttac tcccggcgat ctcttccggt ttgtagtcac cgcctttcac
    17761 cagcaggtct ggcaggatcc cggcgatcag acgctgcggc gtgtcttctt caaacgacac
    17821 tacccagtcg accgcttcca gtgcgcccag cacaatcata cgctgttcga gtgggtttac
    17881 cgggcgggaa tcccctttca gccgtttggt ggaggcatcg ctgttgacgg caacaatcaa
    17941 gcggtcaccc agcttgcggg catttgccag ataagagacg tgcccggcat gtaggatatc
    18001 gaagacaccg ttggtcatta ccactttttc accacgttta cgcgctgcca ctacagccag
    18061 cttcagttcc tcttcggtca tcacgccaaa gccggtatct gcgcgtccac gtacagcgtt
    18121 ttccagctcg atcggcgaaa ccgtggacgt ccccagtttg ccgaccacca caccagccgc
    18181 cgcattggca aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag
    18241 gacgccaatc accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgagttgg
    18301 catatgcagc ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac
    18361 taacagagcc gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc
    18421 ttcggtctta catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt
    18481 agcgccgcgg tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacacccgc
    18541 tttacgcgcc agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga
    18601 aagcaccagc gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg
    18661 atcaacacct tcgaaacctt cttcaaaatc cagacggatc agctgttggt tgcgggaaag
    18721 tacccgtaat ttggtaatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt
    18781 gacgtcggcc agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac
    18841 caggcgtgca ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg
    18901 acgttcttcg atggtattca ctttaaccac gggcaccggc gcttccggcg agatacgact
    18961 ggaggggccg taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg
    19021 ttcaaactct ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc
    19081 gataatacca tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca
    19141 cgctctgcgg tgaagcaatc ctccggcaca tggcccggca attcctgtaa tgccagatga
    19201 tgaagttcat cgcgaagcgt agtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc
    19261 ataatgtcgt tttgcgccag tagttccaga atgcgcacgt tgtctgacca gcgcgttaac
    19321 ttcggttttt catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta
    19381 attccccctt catcagcttt gatatcaaag cgatcgcgat gtttattgcc gagatgagcg
    19441 cgcattttct cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc
    19501 gtcataatct cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact
    19561 acacgcgcac gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatcg
    19621 gcaaatgctt ctgtggaggt aaccagcatt cccgccgccc cggacgggcg cagccgcgca
    19681 tccacttcgt acaaaatgcc ggaagaggtt cgcgtgctga acaggtgcat aatgcgttgc
    19741 gccagacgca gataaaactg tcgtccgtcg atttcccgct cgccgtcagt catcgcatcc
    19801 attgggcaat catggaggaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg
    19861 cccagcttgc cgtagccgac cactgcaaaa ccacgccctt cgcgttcgtt caggtgattc
    19921 ggcttaccgt agcgggcaac catttgaacc cacgcctgct gaacgacggc atctatcatg
    19981 gcttccgcca gccaggttaa gtgatcgctc actttcatca ccggtagcgt accggcgata
    20041 tccgctgcgg cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt
    20101 tgctcttcgt catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca
    20161 tcggtcgccg tcggctggta aagggtgttt ggatcgagca attcatccag caataatgga
    20221 taacgcgcca gctggctggc aatcatcggc gacgctgcac acagagaaat caaatgttta
    20281 agcgccgcgg ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccgcc
    20341 agcaaggcgg taatgcgcaa cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt
    20401 agcagatgcg gcatcagatg gtcgagcacc tgacgcccgc gtggcccgat tgtgcgctta
    20461 tccagctctt tgcggaaatc ggcaatcagc gtcagcacct gtttgcgatc gtcctcgcta
    20521 agatgcgcca gcactggtgt agtgtcatct tcctgcaacg catcctgcca cagctcgcgc
    20581 cactgttccg acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta
    20641 aacacccggc gcacattggt catatgtgcg gtcagcgccc cggtcaattg cggccagtca
    20701 gcaaagtcca tcgcccacgc cagccgcgca cggttaagct catcagaagg aagcgtctgg
    20761 gtttgttcgt cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatacgcc
    20821 actcgcaatt gttcagcatc gttttcagaa agcagatgca gcgcggcaat gacgctgagc
    20881 gttggcagta aagagcgcga ctgcagcgac ggttcgcgcc cgccgcgaat gagctggaac
    20941 acctgaacga taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc
    21001 aaaccacgtc gacgcacttc acgggcaatc attcctttca tgttgcgcag cgactgaatc
    21061 acgctgaaat cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta
    21121 gcatagacgc cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag
    21181 tcgcgcccct gctcctggta ataatcttcc aacgcggcaa agctcagcac cagcgggcca
    21241 ctttcgccaa acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt
    21301 tgatccagca ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt
    21361 tcccagcgtc caccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatca
    21421 gaggagaaat tcagttcccc gccgcccagc ttacccatgc ctaaaatcag cagcggttgc
    21481 gcttcgccct gcgcattgca cggcgttccc cactcgcggc agcaggcgtc atatagccag
    21541 tcacgcgccg caacaatcag cgtctccgcc agatagctga gctgctgcaa tatgctctct
    21601 tcagtaacca gtgccagcgt ttgcgcccag gcgatacgta ccatgatgcg ccgacggaac
    21661 agccgcagct cgcgcattaa cccggcttcg tcactcacat taatgagcgc ctcctgcaac
    21721 catgcaacgt aatgctgcca ttcgtcggcc tgcggcggtt ggctttccag ttccgtcagc
    21781 cactctggat gcgcacttat gctgtcctgc acaaaatcac tgaaagtaag tactgacttc
    21841 gcctgtgcac taagtgattc ctcggctaaa ggctctggca gccgctcaac aatggtctgc
    21901 cagtactgct gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta
    21961 acgttttccg ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc
    22021 aatttcgatg cgttgcccgg tcgcaatagc gtgatgcagc ccctgccagt tctccagcca
    22081 ggcttgcgcg acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg
    22141 cgtcaggcgt ggcaactggt cacggtagcg atcgcctaac ggctggcaga aaacgctttt
    22201 cagttcagcg gcatggcggg aaagatggat atcggcaaag cgtttgaagg agtcgctgat
    22261 tttgccctgc gctttggcat ctaaaaatgg ctgccatgct ttgcttacca accattcggt
    22321 caacgccagc ttcgccattg ccgtttcggt agagtagacc gccgtcacgg cagaaaccgc
    22381 agaagcaatg gtcgcttcgc attgggtcag caaatcacgt aagtgagtgc tcgctttacg
    22441 cggcacaata ccaccgaaca gcatcagcgt atgacggacc aagctaatgg ctgccagcac
    22501 ctgttctttc gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc
    22561 taacgccagc tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg
    22621 caaaatggtg gtcggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg
    22681 cgccgctttg ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag
    22741 tttcagcacc gcgcgcgtgt cgccgctaag cagctccagt tccagctcac agataggttc
    22801 agcaaattcc cccgctttca cctcaccctg atcgagagcg atttcaattc ggctgccatc
    22861 gaccgccacc agccattttt cgcgataaaa atcggtgctg aacagcggtt gtacgcggga
    22921 agcgagatcg gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc
    22981 gagcgtcggt tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac
    23041 tcttcctgca actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc
    23101 catatcgtgc ccacgcagcc agttatccgg cgtttcgtag taaatattca gtaactgcac
    23161 ggggtcatgg tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcgct
    23221 gtgattaaca ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg
    23281 tcacataagt gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt
    23341 ttgcgccaaa ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca
    23401 agacctgaca cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca
    23461 ctactatcgt tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat
    23521 cggattaact ttactcgcac ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta
    23581 tgtttctgac gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt
    23641 gggcacggtt aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta
    23701 tgcccaggtg aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac
    23761 tgagccaagc ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga
    23821 taaactcacc aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt
    23881 ggcgcagagc gacagcgtga tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga
    23941 gctgattgtc gcgcagaaaa aggtcgatgc cgccagcgtg cagctggatg acaaacagcg
    24001 caccatcatc atgcaatggt ttatgtatgg cggcggcgtg ctggggcttg gcttgctgct
    24061 cggtctggta ctgccgcacc tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta
    24121 aatcgccttc ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga
    24181 tggcgtgaag atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt
    24241 caaagacaga gattgggtag tggtcggcag tacgccacag gagatgctcg acgcgggcta
    24301 ccagcaggta ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc
    24361 gctggcacgt accgaacgga aatccggctc cggttacacc ggttttacct gctatgccgc
    24421 accggatgtc acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc
    24481 ccaggacgat aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg
    24541 tctgttgcgc catgtttccc ccgcttttgg cgaagatccg ttacgcgtac tgcgcgtggc
    24601 gcgttttgct gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct
    24661 gatgcgcgag atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa
    24721 agagacagaa aacgccctca ccacccgcaa tccacaggtg ttcttccagg tactgcgcga
    24781 ttgcggcgca ctgcgcgttt tattcccgga aattgacgcc ctgtttggtg ttccggcccc
    24841 tgccaggtgg catccggaaa tcgacacggg tattcatacc ttaatgacgc tctcaatggc
    24901 ggcgatgctg agtccgcagg tcgatgtccg tttcacaact ttatgccacg atctcggtaa
    24961 aggactgacg ccgccagaac tctggccgcg tcatcatggt catggcccgg cgggtgttaa
    25021 gttagtggaa caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag
    25081 actggtggct gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat
    25141 cgtcaaatta tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc
    25201 gctgaccagc gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca
    25261 aggccgctgg ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagccgtcgt
    25321 tgaagcggga tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc
    25381 ggtagccagc tggaaggaac aacgttgccc aaagcctgag tgagtattgg gcattttgcc
    25441 ggatgcggcg taaacgcctt atccggctac atgtcaacga cagttgtagg cctgataaga
    25501 cgcgcaagcg tcgcatcaag cgttgattgc cggacgcggc gtgaacgcct tattcggcct
    25561 acgcaatcgt ggacaacgcc aggcatccga cactattcct caattaaaag aacacgacat
    25621 acaccgcagc cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa
    25681 tcaattgcag gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa
    25741 cggcaaacat cgggatatcg ccgcttgtca ggaagcccca gcttttgtag agatcgagcg
    25801 ccgttgcgcc catcatcatc ggcaccgcca gcaggaacga aaactcggaa gcagcgtaac
    25861 ggctcacccc catcagcatc ccacctgaaa tggtcgcccc ggaacgggag aaacccggcc
    25921 acagcgccag acactggaaa caaccaatca taaacgcctg acgataggtc atatcatcaa
    25981 gacccggcgc acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc
    26041 caacgaccag cgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga
    26101 acaacagccc cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac
    26161 gacctttgct ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa
    26221 acagacgccg ccagaacatc actactaccg ccagaattga tcctaactgg atcacaactt
    26281 caaaggtttt cgccgtgtcg ccctcaaacc ccaacaagtg accgacaata atcatatggc
    26341 ccgtgctgga taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta
    26401 tcagcagcga gtgcatatcg ctcatcaata aacccctaaa ttataaaaat gtaacgcttg
    26461 tccgaactac tgcagtatga ccaggttata accgtttggt ttaacagctg taaaattaat
    26521 tattttcttt cagattattg ccacgctcaa tgattacgcc aacattcgcc gcccgcgcca
    26581 ctgcgcctgg cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca
    26641 gctctgccac ctcttccgcc acgcgctcca ccagcgcaaa acgtgccccc tcgacgtggc
    26701 tgaccaccgt ttctgcaatg tcagcgtagc tgaggcaatc cgccacatta tcgctttttg
    26761 ccgctttacg gttatcccac gccatttcga tatcgaacac taatttctgt tcgatggtct
    26821 gttcccagtc gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat
    26881 ccatcacgac ctgctcgctt tttggctaac ccggatacca cttccggcga aatgtgcgta
    26941 ttatccacag attcatcgtt taacacgaat tttcaaaacg gaacagctta tgagtgcaat
    27001 cgcgcctgga atgatcctca tcgcgtacct cagcggctcc atttccagtg ccattctggt
    27061 ttgccgcctg tgtgggctgc ccgatccgcg aaccagcggc tccggtaatc ctggcgcaac
    27121 taacgtgtta cgtataggtg gcaaaggagc agccgtagca gtactgattt tcgacgttct
    27181 gaaaggaatg ttgcccgtct ggggcgcgta tgaattaggt gtcagcccct tctggctagg
    27241 cttaattgcc atcgccgcct gtcttggaca catctggccc gttttcttcg gatttaaagg
    27301 aggaaaaggc gttgctaccg cttttggtgc catcgcaccc attggctggg atctcaccgg
    27361 agtaatggcg ggaacctggt tactgaccgt gctattgagc ggatactcgt cgctgggagc
    27421 gattgtcagt gcactgattg ctccgtttta tgtctggtgg tttaagccac aattcacctt
    27481 cccggtttcg atgctctctt gcctgatcct gctgcgtcat catgacaaca tccagcgtct
    27541 gtggcgtcgt caggagacaa aaatctggac gaaattcaaa agaaagcgcg aaaaggatcc
    27601 cgagtgattt ctggtggatc tacatgacct gatagccttc atcgggtttg cccagccgtt
    27661 gctggcacca tgccgccaga aattccacgc agacacgtaa tttcatgctg cgatagagcg
    27721 gctcccggta aacagcccag atattggcgc tttgtgcata ctccggcaat acttgcacca
    27781 gtttgccact ctccagaaac ggcaacacat cccactcgga acgcagcata atccctttgc
    27841 cctccagcgc ccattgcagc acaatctcgc cgctattgga ggaaagatgc ccgctaacct
    27901 tcaccgattt tttctcctga ccgttcccca actcccatat tccgtgggtc atatcgcgct
    27961 ctttggtcac cagacagtca tgacgactta attcctgtag ggattgtggc tgcggatatt
    28021 tttgcagata ctcaggtgct gcgcataata ttcttttatt tttcgttaac agatgcgcaa
    28081 tataataatc aggaatttcg tcattaatac gaatatccag atcaatatta tcctgcacta
    28141 aatcaatttg ccgatcgaac aattcaaaat acacctgtaa ctcaggataa ttgcgcataa
    28201 gttcggtaat ggctggcgca atatggctgc gcccaaaacc aaaactgcaa ccaatacgaa
    28261 tcatcccctc cgggcgcgtt ttgatttgcg tgacgtcatc gaccagtcgc tgatattggg
    28321 tgaggatttc cagcgcatgt tcgtaacaac gttggccact ttcagtcagc gccacgccgc
    28381 gagcggagcg gttcagcagc gttgttgcca gcgtattttc aaggatttgg atccgcttgg
    28441 tgacaaatgc aggcgtttgc ccaagcgtcg ccgctgcagc actaaaactc ccgctgtgga
    28501 cgatttctac cagtacctgc aagtctttgg ctaaaggcca gctattcagc atcgttaatt
    28561 atccgcagtt gtgataagcg cagtgtattt cgcaaaacat tgccattccc tgctctggcg
    28621 caatattcat tcttattttc gcgttgattc acgaactgtt aattacatat tcacggtggc
    28681 aaaaaatata aaaccacatt tttagtggta gtttgtggcg gtgaattttt ccagacaaat
    28741 acaaaaactg gagttgccat gatgagcgaa agtaataagc aacaggcagt gaataagttg
    28801 acggagattg tcgctaactt taccgccatg atttctaccc gaatgcctga tgacgtggtg
    28861 gataaactaa aacagctaaa ggatgccgaa acgtcgtcga tggggaaaat tatctaccat
    28921 acgatgttcg acaacatgca aaaagcgatt gacctgaatc gtcctgcctg tcaggacacc
    28981 ggggagatta tgttcttcgt taaagtcggt tcccgcttcc cactgcttgg cgagctgcaa
    29041 agcatactca aacaagccgt ggaagaggcg accatcaaag cgccgctgcg tcacaatgcg
    29101 gtagaaattt ttgacgaagt aaacaccggc aaaaataccg gtagcggcgt accgtgggtc
    29161 acctgggata tcgtccccga cggtgacgat gcggaaatcg aagtttacat ggcaggcggc
    29221 ggctgcacgc taccaggccg ctcgaaagtg ttaatgccgt cagaaggcta cgaaggcgtg
    29281 gtgaaattcg tcttcgaaaa tatctccacc ctcgcagtaa acgcctgtcc accggtactg
    29341 gtgggcgtgg gcatcgccac ctcggtggaa accgccgccg tactctcgcg taaagccatt
    29401 ttgcgcccga ttggcagccg ccaccccaat ccaaaagcgg cagagctgga gctacgcctg
    29461 gaagaaggac tcaaccgtct ggggattggt ccacaagggc tgactggcaa cagttcagtg
    29521 atgggcgtgc atatcgaatc tgccgcccgc catccgtcaa ccatcggcgt tgctgtttct
    29581 accggttgct gggcgcatcg tcgcggcaca ctgctggttc atgccgatct cacctttgaa
    29641 aatctgtctc acacccggag cgcgttatga aaaagatcct gacaaccccg atcaaagctg
    29701 aagatctgca agatattcac gtcggcgatg tgatctatct gaccggtacg ctggtgacct
    29761 gccgtgacgt ttgtcaccgc cgattgatcg aactgaaacg tccgctccct tacgatctca
    29821 acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac aaatgggaga
    29881 tggtctccgt cggtccgaca accagtatgc gtatggaaag ttttgaacgt gaatttatcg
    29941 agcagaccgg cgtgaaactg gtggtcggca aaggcggaat ggggccgctg accgaagaag
    30001 gctgtcagaa attcaaggcg ctacatgtaa ttttcccggc aggctgcgcg gtgctggcgg
    30061 caacccaggt ggaagagatt gaagaagtgc actggacaga actcgggatg ccggagtcac
    30121 tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat acccacggca
    30181 acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc atcgtggaag
    30241 agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctcg ccccctctcc
    30301 tttttcgcag gcataacacg atgaaacctt ccactgaatg gtggcgatac cttgcgccgc
    30361 tggcggtcat cgccattatt gctctaattc cggttcccgc agggctggag agtcatacct
    30421 ggctctactt tgccgttttt actggcgtaa tcgttggact gatcctcgaa cccgtgccgg
    30481 gtgccgtggt ggcgatggtg ggtatctcca tcatcgccat actctctccc tggctgctgt
    30541 tcagcccgga gcagctcgct cagccaggct ttaaattcac tgcaaaatcc ctctcgtggg
    30601 ccgtttccgg tttttctaat tcggttatct ggctgatttt cgccgccttt atgtttggca
    30661 caggctatga aaaaaccggg ctggggcgac gtatcgcgct gattctggtg aaaaagatgg
    30721 ggcatcgcac gctgtttctt ggctatgcgg tgatgttctc cgagctgatc ctggcacctg
    30781 tcacaccgtc caactcggcg cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc
    30841 cgccgctcta tcaatcacaa ccaaacgaca gcagttcgcg cagtattggc tcgtacatca
    30901 tgtggatggg gattgttgcc gactgcgtga ccagcgccat tttcctgacg gcgatggctc
    30961 ctaacttgct gttaattgga ctgatgaaaa gcgcatctca cgccacgctg agttggggcg
    31021 actggttcct cgggatgttg ccgctcagta ttttactggt cctgctggtt ccctggctgg
    31081 cttacgtgct gtacccgcct gtactgaagt caggcgatca ggtgccgcgc tgggcagaga
    31141 cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg
    31201 taggcgcgct ggtgctgtgg attttcggcg gtgattatat cgatgccgcg atggtcggtt
    31261 acagcgtggt ggcgctgatg ctgcttctgc gcattatcag ttgggacgat attgtcagta
    31321 ataaagcggc gtggaacgtt ttcttctggc tggcctcgct tatcaccctc gcgaccggac
    31381 tcaacaacac cggttttatt agctggtttg gcaaactgtt agcaggcagc ttaagcggtt
    31441 attcgccaac gatggtgatg gtggcgttga ttgtggtgtt ttatctactg cgctactttt
    31501 ttgccagcgc cacggcgtat acctccgctc tcgcaccgat gatgattgcc gctgcgctgg
    31561 cgatgccgga aatcccgctg ccggtgttct gcctgatggt tggtgcggca attggtctgg
    31621 ggagcattct tacaccatac gccaccggcc ccagtccgat ttactacggc agtggttatc
    31681 tgccaacggc ggattactgg cgactggggg cgatttttgg gctgatattc ctcgtattgc
    31741 tggtgattac cggcttactg tggatgcccg tggtgttgct ttaagtaagg acggtgccgg
    31801 aagtaactct ccggcacccg atagttttac gcagccggta actccgccag cggccagcgc
    31861 ggacgcacgc taacgccgag atccgccgtc gcgcctgctt taaaccgcac cattccggca
    31921 taggcgatca tcgcgccgtt atcagtacaa aattccggac gcgcgtagaa cacttcgccg
    31981 cggcgttttt tcatcatttc agccagcttc gcccgtaacg tgcggttagc actcacgccg
    32041 cccgccatga ccagtcgctt aaagcccgtc agatccagcg cacgcttaca cttaatcatc
    32101 aacgtatcga ccaccgcatc ttcaaaggcg cgggcgatgt cagcacgcgt ctggtcgtcg
    32161 gtgccgttgt cacgaatggt atttgccgcg aaggttttca ggccggagaa gctgaaatcc
    32221 agccccggac ggtcggtcat cggacgcggg aaaacaaagc gcccggcagt accctgcgcc
    32281 gccattttcg acagtaacgg tccgccagga taatccagcc ccagcagctt cgcggtttta
    32341 tcaaacgctt caccggcggc atcatcgata gactcgccga gcagctcgta ctgaccaatg
    32401 ccagtcacgc tgattaactg cgtatgaccg ccggaaacca gcagcgcaac aaacggaaat
    32461 tccggcgggt tatcttccag catcggcgct aacagatgcc cttccatatg gtgtacaggg
    32521 atcgccggaa cgttccaggc aaacgccaga gaacgcccca cggtcgcgcc aaccagcagt
    32581 gcgccgacta atccagggcc tgcggtatag gccacagcat caatgtcttt tgccgttaaa
    32641 ccagactcct ttagcgccgc ctggatcaac ggtacggttt tacgcacatg atcgcgggag
    32701 gccagttcag gcacaacgcc cccgtagtca gcgtgcaatt tcacctgact atacaattgg
    32761 ttggctaaca aacctttttc atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt
    32821 tcaataccca gtacacgcat gacttgtttt acctcgcttt attaccgcgc agtgtaggac
    32881 caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa agcctcgtgt atactcctca
    32941 cccttataaa agtccctttc aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca
    33001 gtaaaattcc gcaccatttt gaaataagct ggcgttgatg ccagcggcaa accgaattaa
    33061 tcaaaggtga gaggcacatg ccggtaatta aagtacgtga aaacgagccg ttcgacgtag
    33121 ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc
    33181 gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc taaagcttct gcagtgaaac
    33241 gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac tcgtctgtac taattccccg
    33301 agagcgttgc tctccgatca gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat
    33361 gcgcggctta ttttcgttta tgaattgcta aaaatcgggg cctatggctg gacgaatccc
    33421 acgcgtattc attaatgatc tgctggcacg cactgacatc gtcgatctga tcgatgcccg
    33481 tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa
    33541 aaccccgtcc ttcaccgtta acggtgagaa acagttttac cactgctttg gatgtggcgc
    33601 gcacggcaac gcgatcgact tcctgatgaa ctacgacaag cttgagttcg tcgaaacggt
    33661 cgaagagctg gcggcaatgc acaatcttga agtgccattt gaagcaggta gcggccccag
    33721 ccagatcgag cgccatcagc ggcaaacgct ttatcagttg atggacggtc tgaatacgtt
    33781 ttaccaacaa tctttacaac aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg
    33841 cggattaagc cacgaggtta tcgctcgctt tgcgattggt tttgcgcctc ccggctggga
    33901 caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg
    33961 gatgttggtc actaacgatc agggacgcag ttacgatcgt ttccgcgagc gggtgatgtt
    34021 ccccattcgc gataaacgcg gtcgggtgat tggttttggc gggcgtgtgc tgggcaacga
    34081 tacccccaaa tacctgaact cgccggaaac agacattttc cataaaggcc gccagcttta
    34141 cggtctttat gaagcgcagc aggataacgc tgaacccaat cgtctgcttg tggtcgaagg
    34201 ctatatggac gtggtggcgc tggcgcaata cggcattaat tacgccgttg cgtcgttagg
    34261 tacgtcaacc accgccgatc acatacaact gttgttccgc gcgaccaaca atgtcatttg
    34321 ctgttatgac ggcgaccgtg caggccgtga tgccgcatgg cgagcgctgg aaacggcgct
    34381 gccttacatg acagacggcc gtcagctacg ctttatgttt ttgcctgatg gcgaagaccc
    34441 tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat
    34501 gccgctttcc gcatttctgt ttaacagcct gatgccgcaa gttgatctga gtacccctga
    34561 cgggcgcgca cgtttgagta cgctggcact gccattgata tcgcaagtgc cgggcgaaac
    34621 gctgcgaata tatcttcgtc aggaattagg caacaaatta ggcatacttg atgacagcca
    34681 gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt tctcgccctg ttccgcagct
    34741 aaaacgcacg accatgcgta tacttatagg gttgctggtg caaaatccag aattagcgac
    34801 gttggtcccg ccgcttgaga atctggatga aaataagctc cctggacttg gcttattcag
    34861 agaactggtc aacacttgtc tctcccagcc aggtctgacc accgggcaac ttttagagca
    34921 ctatcgtggt acaaataatg ctgccaccct tgaaaaactg tcgatgtggg acgatatagc
    34981 agataagaat attgctgagc aaaccttcac cgactcactc aaccatatgt ttgattcgct
    35041 gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc acgcatggtt taagcaacga
    35101 agaacgcctg gagctctgga cattaaacca ggagctggcg aaaaagtgat ttaacggctt
    35161 aagtgccgaa gagcgatcgg gaagcccccg acagccgcac tgagaggcag cggcaaatat
    35221 ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc gttggatttc agcgttaacg
    35281 gctgaaggac atcgggtcaa tcgcccaaca ccaacctcat gaaataagtg tggataccgt
    35341 cttatggagc aaaacccgca gtcacagctg aaacttcttg tcacccgtgg taaggagcaa
    35401 ggctatctga cctatgccga ggtcaatgac catctgccgg aagatatcgt cgattcagat
    35461 cagatcgaag acatcatcca aatgatcaac gacatgggca ttcaggtgat ggaagaagca
    35521 ccggatgccg atgatctgat gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc
    35581 gccgcgcagg tgctttccag cgtggaatct gaaatcgggc gcacgactga cccggtacgc
    35641 atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc gcgaaggcga aattgacatc
    35701 gctaagcgta ttgaagacgg gatcaaccag gttcaatgct ccgttgctga atatccggaa
    35761 gcgatcacct atctgctgga acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc
    35821 gatctgatca ccggctttgt tgacccgaac gcagaagaag atctggcacc taccgccact
    35881 cacgtcggtt ctgagctttc ccaggaagat ctggacgatg acgaagatga agacgaagaa
    35941 gatggcgatg acgacagcgc cgatgatgac aacagcatcg acccggaact ggctcgcgaa
    36001 aaatttgcgg aactgcgcgc tcagtacgtt gtaacgcgtg acaccatcaa agcgaaaggt
    36061 cgcagtcacg ctgccgctca ggaagagatc ctgaaactgt ctgaagtatt caaacagttc
    36121 cgcctggtgc cgaagcagtt tgactacctg gtcaacagta tgcgcgtcat gatggaccgc
    36181 gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag
    36241 aaaaacttca ttactctgtt taccggcaac gaaaccagcg atacctggtt caacgcggca
    36301 attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc
    36361 gccctgcaga aactgcagca gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa
    36421 gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg
    36481 gttgaagcga acttacgtct ggttatttct atcgccaaga aatacaccaa ccgtggcttg
    36541 cagttccttg acctgattca ggaaggcaac atcggtctga tgaaagcggt tgataaattc
    36601 gaataccgcc gtggttacaa gttctccacc tacgcaacct ggtggatccg tcaggcgatc
    36661 acccgctcta tcgcggatca ggcgcgcacc atccgtattc cggtgcatat gattgagact
    36721 atcaacaaac tcaaccgtat ttctcgccag atgctgcaag agatgggccg tgaaccgacg
    36781 ccggaagaac tggctgaacg tatgctgatg ccggaagaca agatccgcaa agtgctgaag
    36841 atcgccaaag agccaatctc catggaaacg ccgatcggtg atgatgaaga ttcgcatctg
    36901 ggggatttca tcgaggatac caccctcgag ctgccgctgg attctgcgac caccgaaagc
    36961 ctgcgtgcgg caacacacga cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg
    37021 cgtatgcgtt tcggtatcga tatgaacacc gatcacacgc tggaagaagt gggtaaacag
    37081 ttcgacgtta cccgtgaacg tatccgtcag atcgaagcga aggcgctgcg caaactgcgt
    37141 cacccgagcc gttctgaagt gctgcgtagc ttcctggacg attaatcggt aggccggatc
    37201 aggcgttacg ccgcacccgg cacctgtccc tttgcacaaa cgccaccttt tcggtggcgt
    37261 tttttatcgc ccacgcacca ccagcgcctg gtccagctcg cgatacgctt caaccagttt
    37321 ctccagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct gcgtcgaacc
    37381 aatgctgagc gtttgtttcc cccactgtgc accacgctgg ctgaatccct gttcatatgc
    37441 ttgtttgccc agaatcgcca acgcttgcgg ctgataatct tcaatttttt caatcagctt
    37501 acgcccacct gcgtgtagct cttgctttga aatttcattg gcttgcaccg ttggcctgtc
    37561 taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct gcggcttcaa
    37621 ctgatggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg ccggatgagc
    37681 aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa acacgacccg
    37741 taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc accaggaaaa
    37801 gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcgcggta atggctggat
    37861 tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt ggttagagca
    37921 ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca gatatagcaa
    37981 aggctgacga gaaatcgtca gcctttttct ttttatatat cagttacttt gcgtgccagt
    38041 aggccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg cggcttaaat
    38101 tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca gggatttgca
    38161 tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg atattaaaac
    38221 catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc agcgcactaa
    38281 cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcaat gcgggcattc
    38341 cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc agcgaaccgc
    38401 gcggacctgc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag ccgctggcga
    38461 ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagcg
    38521 gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc tcttccgtta
    38581 ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa
    38641 agccgcgcga tgtaaaaccg tccagcgcct cgccgccaag gacaatgcgc tgaaaaccgg
    38701 cgctgatacg ctcaacgcgt agcacagtca attcacggaa gcgcagatca ttgcgaacgc
    38761 gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta atcagatata
    38821 tctaaataat tttcgcaaat gataatgatt gttaatcatg ataaatgcaa gcgatttgta
    38881 gaactgatat gtctatagtc tgacaagacg aaccgcctct tctcaggcat cattactcaa
    38941 cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc acatttaaaa
    39001 atcattttta ttcttgcaag agtgctcata tcagatataa attagatata tctaattaag
    39061 caaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga aggccagcca
    39121 cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg acacggtcac
    39181 cagcatgaac acggtcaatg ttgcggtggt cgccacggtc gcggcggtgg tcgtcggcaa
    39241 cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc gcgcgatgac
    39301 agccacggtt acgaattgat taaagcgatt gagaatctaa cccaagggaa ttacacccca
    39361 agcccgggcg tcatctaccc gacgctggat tttctgcagg agcagtcgct gattaccatc
    39421 cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc gcagtggctg
    39481 gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg ttgcgttggc
    39541 gctgcgctgc gccagaaccc gcaaatgaag cgggcgctag ataattttaa agcggtgctg
    39601 aatttacgcg tcaaccagag tgatatcact gatgcacaaa taaaaaagat cattgcggtg
    39661 atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc gccagtggcg
    39721 cggtgcaatt gccggatgcg acgcttgacg caccttatcc ggcctacacc cgctacacac
    39781 cccgtaggcc tgataagatg cgccagcatc gcatcaggca tcgtgtatca aataccggat
    39841 ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt
    39901 caccatcgcc gacacctgcg cactctcttc caccagctcg gcatttttct gggtgatgag
    39961 gttaagctca tccactgcac gggtcaggct ggaaagacca tcggcctgtt ccagcgttga
    40021 atggctaatc tgggcaatca actgggtgac gtttttcacc tgtgccacaa tatcttccat
    40081 cgtacgtccg gcggcgtgta cctgctgcga accggattgc accttatcag cactggcatc
    40141 aatcagcttg cgaatatcgt tggcagcatt ggcgctgcgg ctggctaaat gacgcacttc
    40201 ccccgccacc accgcaaaac ctttgccctg ttcgccggca cgcgccgctt ccaccgccgc
    40261 attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag taatggtgcc
    40321 aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg tcatcgcctc
    40381 cccgccctgc actgccgcat tactggcggt gattgacagt ttatcggcag ccgacgccgt
    40441 ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg tttgctgaac
    40501 gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc
    40561 actgccgttt ctgacactgg acacctggct tgagacatcg ttaattaacc aacggcacat
    40621 caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc gattcagatg
    40681 ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga cattttctat
    40741 cgggcggaca atctgccatt caaaacaggc gcttgccaac aataccacta acgcacagag
    40801 gatatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca agataaacat
    40861 cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt tacccagcca
    40921 gcctttacgc accaccaggc ctttatgaat acgcttaccg gtacgtccgg cgttcagcgc
    40981 tttgtacagc ggctccaccg ccgcgatctc ttcatccgtc gcccgggtac gaatcgacat
    41041 atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca cccaataatg
    41101 gtcaccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt ttttcagggt
    41161 gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca tgttgtgcgg
    41221 ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt cattagcatg
    41281 agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg ccagcggggt
    41341 attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctgtgcag gttatatggt
    41401 tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa ccgattaatt
    41461 tgatttagat cgcaatttgc gattaaagca caaatctaat tcgttgattt aaaatacttt
    41521 cactctgtta ctatacgaaa acgttaatta tcttgcccaa aaatcaggca gttaatgccc
    41581 tgaaagcgtg catttgcgca gcaattatca aatccatacc cgacaaaaac cgtgcaaaat
    41641 aacaacagat gttaacaaat agcattaaat attgcgcaaa tgataaccac atttgtgttt
    41701 atcccgattt tcgcgatcgc agccggagtg gcgcaatccc tgcaatactt aaatcggtat
    41761 catgtgatac gcgagcctcc ggagcatatt ttgaacaggt taccttcgag cgcatcggct
    41821 ttagcgtgca gcgcccacgc cctgaatctc attgaaaagc gaacgctgga tcatgaggag
    41881 atgaaagcac ttaaccgaga ggtgattgaa tacttcaaag agcatgtcaa tccggggttt
    41941 ttagagtatc gcaaatctgt taccgccggc ggggattacg gagccgtaga gtggcaagcg
    42001 ggaggtttaa atacgcttgt cgacacccag ggacaggagt ttatcgactg cctgggaggt
    42061 tttggaattt tcaacgtggg gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa
    42121 cttgcgaaac aaccactgca cagccaggag ctactcgatc cgttacgggc tatgctggcg
    42181 aaaacccttg ctgcgctaac gccaggtaaa ctgaaataca gcttcttctg taatagcggc
    42241 accgagtccg ttgaagcggc gctgaagctg gcgaaggctt atcagtcgcc gcgcggcaaa
    42301 tttactttta ttgccaccag cggcgcgttc cacggtaaat cacttggcgc gctgtcggcc
    42361 acggcgaaat cgaccttccg caaaccgttt atgccactgc tgccagggtt ccgtcatgtg
    42421 ccgtttggca atatcgaagc catgcgcacg gctcttaatg agtgcaaaaa aaccggtgat
    42481 gatgtggccg cggtgatcct cgaaccgatt cagggcgaag gtggcgtaat tctgccgccg
    42541 ccgggctatc tcaccgccgt gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc
    42601 gatgaagtac aaacgggcat ggggcgcacg ggcaagatgt tcgcctgcga acatgagaac
    42661 gtacagccgg acatcctgtg cctggcaaaa gccctcggcg gcggtgtgat gccgattggc
    42721 gcaaccatcg ccactgaaga ggtgttctca gttctgttcg acaacccgtt cctgcatacc
    42781 accacctttg gcggcaaccc gctggcctgt gcggcggcgc tggcgaccat caatgtgttg
    42841 ctggagcaga acttaccggc tcaggctgag caaaaaggcg atatgttgct ggacggtttc
    42901 cgtcaactgg cgcgggaata tcccgatctg gtacaggaag cgcgtggtaa agggatgtta
    42961 atggcgattg agtttgttga taacgaaatc ggctataact ttgccagcga gatgttccgc
    43021 cagcgcgtac tggtggccgg aacgctcaat aacgccaaaa cgatccgcat tgaaccgcca
    43081 ctgacactga ccattgaaca gtgtgaactc gtgatcaaag cggcgcgtaa ggcgctggcg
    43141 gccatgcgag taagtgtcga agaagcgtag tatcaaatcg gatggcaata cgacgtcgcc
    43201 atccgatctt tttatacgat acgtacgccc gcaggcatca tccgctccgg cgttaacagc
    43261 acgctttcac tgccgtcatc ggtttccgca cacaacagca tgcattccga cgtttcacca
    43321 cgcattttcg ctttttgcag attgcataag accaccaccg tttttcccat caactcttct
    43381 tcgctgtagt acggcaccag gctggtcacg gtttgcagtg ttttttcccc cacatcaacc
    43441 tgtacgatgt acagcttgtc ggcgttttca tggcgtttca cttccacaat ctttccgacg
    43501 cgcatttcca gacgtgcaaa atcagcgtaa gccacggttt ccattgcttc ctcccttcga
    43561 gtaaaatttt actaaactat agcaaaagtt tttctcaatc ctgtaggcta aaaatggaga
    43621 atgcaggcgt gatcacattc gtgagccgct gtgttaccgt tacagcgtca aagaaacgcg
    43681 ctttatttac tgaaaacagg tgacccgata agcacttcct ctacaatggg ggcgcacatc
    43741 agggaaagta aaaaaggtaa acatggcaac actaaaagac atcgcaatcg aagctggcgt
    43801 atccctggcg acagtatcca gggtcttaaa tgacgatccg acattgaatg tgaaagaaga
    43861 gacgaaacat cgcattctcg agatcgccga aaagctggag tacaagacca gtagcgcccg
    43921 taaactacag acaggcgcag tcaaccaaca ccatattctg gctatctaca gctaccagca
    43981 ggagctggag atcaacgatc cttactatct ggcgatccgc cacggcattg aaactcagtg
    44041 cgaaaagctg gcgatcgaac tgaccaactg ttatgaacac agcggattac cagacattaa
    44101 aaacgtcacc ggtattttaa ttgtcggcaa acccacgccc gccctgcgcg ccgctgccag
    44161 cgcgttgacc gacaatatct gttttatcga ctttcacgaa cccggcagcg gttacgatgc
    44221 ggtggatatc gatctggcac gcatcagtaa agaaatcatc gacttctata tcaaccaggg
    44281 cgttaatcgt attggtttta ttggcggtga agatgagcct ggcaaggcgg atattcgtga
    44341 ggtcgccttt gcggaatatg gccgactgaa acaagtggta cgcgaagagg atatctggcg
    44401 cggcggtttt tccagttcgt cgggttatga actggcaaaa caaatgctgg cgcgggaaga
    44461 ctatccgaag gcactgtttg ttgcttccga ttccattgct atcggcgtac tgcgggcgat
    44521 tcatgaacgt ggtctgaaca tcccacagga tatttcgctt atcagcgtta acgatatccc
    44581 caccgcgcga tttacctttc cgccgctctc caccgtgcgc atccattccg aaatgatggg
    44641 aagtcagggc gtaaacctgg tgtatgaaaa agcccgcgat ggtcgcgcgc tgccgctgtt
    44701 agtcttcgtt cccagcaaat taaaactgcg cggcacgacc cgttaaatcc ccttacacac
    44761 tgtccggcaa tcgtttttgc cgggcagtgc tgccgtttat tttcgtgatc cagttaaagt
    44821 aaatgcattt acccgctact ttttagtaaa aattttacta aactccccag caattacaca
    44881 aactaccatc accatgaatg gttccgattt ctctctaccg ggaggcccta tgaatcgctg
    44941 ggaaaacatt cagctcaccc acgaaaaccg acttgcgccg cgtgcgtact ttttttcata
    45001 tgattctgtt gcgcaagcgc gtacctttgc ccgcgaaacg agcagcctgt ttctgccctt
    45061 aagcggtcag tggaatttcc acttttttga ccatccgctg caagtgccag aagccttcac
    45121 ctctgagtta atggctgact gggggcatat taccgtcccc gccatgtggc aaatggaagg
    45181 tcacggcaaa ctgcaatata ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt
    45241 tcccagcgat aacccaaccg gtgcctatca acgtattttc accctcagtg acggctggca
    45301 gggtaaacag acgctgatta aatttgacgg cgtcgaaacc tattttgaag tctacgttaa
    45361 cggtcagtat gtgggtttca gcaagggcag tcgcctgacc gcagagtttg acatcagcgc
    45421 gatggttaaa accggcgaca acctgttgtg tgtgcgcgtg atgcagtggg cggactctac
    45481 ctacgtggaa gaccaggata tgtggtggtc agcggggatc ttccgcgatg tttatctggt
    45541 cggaaaacac ctaacgcata ttaacgattt cactgtgcgt accgactttg acgaagccta
    45601 ttgcgatgcc acgctttcct gcgaagtggt gctggaaaat ctcgccgcct cccctgtcgt
    45661 cacgacgctg gaatataccc tgtttgatgg cgaacgcgtg gtgcacagca gcgccattga
    45721 tcatttggca attgaaaaac tgaccagcgc cagctttgct tttactgtcg aacagccgca
    45781 gcaatggtca gcagaatccc cttatcttta ccatctggtc atgacgctga aagacgccaa
    45841 cggcaacgtt ctggaagtgg tgccacaacg cgttggcttc cgtgatatca aagtgcgcga
    45901 cggtctgttc tggatcaata accgttatgt gatgctgcac ggcgtcaacc gtcacgacaa
    45961 cgatcatcgc aaaggccgcg ccgttggaat ggatcgcgtc gagaaagatc tccagttgat
    46021 gaagcagcac aacatcaact ccgtgcgtac cgctcactac ccgaacgatc cgcgttttta
    46081 cgaactgtgt gatatctacg gcctgtttgt gatggcggaa accgacgtcg aatcgcacgg
    46141 ctttgctaat gtcggcgata tcagccgtat taccgacgat ccgcagtggg aaaaagtcta
    46201 cgtcgagcgc attgttcgcc atattcacgc gcagaaaaac catccgtcga tcatcatctg
    46261 gtcgctgggc aatgaatccg gctatggctg taacatccgc gcgatgtacc acgcagcgaa
    46321 ggcgctggat gacacgcgac tggtgcatta cgaagaagat cgcgatgctg aagtggtcga
    46381 tattatttcc accatgtaca cccgcgtgcc gctgatgaat gagtttggtg aatacccgca
    46441 tccgaagccg cgcatcatct gtgaatatgc tcatgcgatg gggaacggac cgggcgggct
    46501 gacggagtac cagaacgtct tctataagca cgattgtatt cagggacatt atgtttggga
    46561 gtggtgcgac cacgggatcc aggcgcagga tgacaacggc aatgtctggt ataaattcgg
    46621 cggcgactac ggcgactatc ccaacaacta taacttctgt cttgatggtt tgatctattc
    46681 cgatcagacg ccgggaccag gcctgaaaga gtacaaacag gttatcgcgc cggtaaaaat
    46741 ccacgcgctg gatctgactc gcggcgagct gaaagtcgaa aataaactgt ggtttaccac
    46801 gctggatgac tacaccctgc acgcagaggt gcgcgccgaa ggtgaaacgc tcgcgacgca
    46861 acagattaaa ctgcgcgacg ttgcgccgaa cagcgaagcc cccttgcaga tcacgctgcc
    46921 gcagctggac gcccgcgaag cgttcctcaa cattacggtg accaaagatt cccgcacccg
    46981 ctacagcgaa gccgggcatt ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc
    47041 gcagcctgta cctttcgcac cgaataacgc ccgtccgctg acgctggaag acgatcgttt
    47101 gagctgtact gttcgcggct acaactttgc gatcaccttc tcaaaaacga gtggtaaacc
    47161 gacatcctgg caggtaaatg gcgaatcgct gctgacccgc gagccaaaga tcaacttctt
    47221 caagccaatg atcgacaacc acaagcagga gtacgaaggg ttgtggcagc cgaatcattt
    47281 gcagatcatg caggaacatc tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt
    47341 gatcatcagc cgcacggtta tagcaccgcc ggtgtttgac ttcgggatgc gctgtaccta
    47401 catctggcgc atcgctgcag atggccaggt taacgtggcg ctttccggcg agcgttacgg
    47461 cgactatccg cacatcattc cgtgcatcgg tttcaccatg gggattaacg gcgaatacga
    47521 tcaggtggcg tattacggtc gtggaccggg cgaaaactac gccgacagcc agcaggctaa
    47581 catcatcgat atctggcgca gcaccgtcga tgccatattc gagaactatc ccttcccgca
    47641 gaacaacggc aaccgtcagc atgtccgctg gacggcactg actaaccgcc acggcaacgg
    47701 tctgctggtg gttccgcagc gcccaattaa cttcagcgcc tggcactata cccaggaaaa
    47761 catccacgct gcccagcact gtaacgagct gcagcgcagt gatgacatca ccctgaatct
    47821 cgaccaccag ctgcttggcc tcggctccaa ttcctggggc agcgaggtgc tggactcctg
    47881 gcgcgtctgg ttccgtgact tcagctacgg ctttacgttg ctgccggttt ctggcggaga
    47941 agctaccgcg caaagcctgg cgtcgtatga gttcggcgca gggttctttt ccacaaattt
    48001 gcacagcgag aataagcaat gaggatcatc gataacttag aacagttccg ccagatttac
    48061 gcctctggta agaagtggca acgctgcgtt gaagcgattg aaaatatcga caacattcag
    48121 cctggcgtcg cccactccat cggtgactca ttgacttacc gcgtggagac agactccgcg
    48181 accgatgcgc tatttaccgg gcatcgacgc tattttgaag tgcattacta cctgcaaggg
    48241 cagcaaaaaa ttgaatatgc gccgaaagag acattacagg tcgtggaata ttatcgtgat
    48301 gaaactgacc gtgaatattt aaaaggctgc ggagaaaccg ttgaggtcga cgaagggcaa
    48361 atcgttattt gcgatatcca tgaagcatat cggtttatct gcaataacgc ggtcaaaaaa
    48421 gtagttctca aagtcaccat cgaagatggt tatttccata acaaataaca actacggcgg
    48481 caaaaggagt ttgccgccac cgctacccta ctcattttcg gagatgtgtt atgtctgata
    48541 ccaaacgtaa tacaatcggc aaattcggct tgctctcgct gacttttgcc gccgtttaca
    48601 gctttaacaa cgttatcaat aataatattg agcttggact ggcctcggca ccgatgtttt
    48661 tcctcgcgac gattttttat tttattccct tctgtctgat catcgcagaa tttgtttcgt
    48721 taaataaaaa ctcagaagcc ggtgtctacg cgtgggtaaa aagttcgctg ggcggacgtt
    48781 gggcatttat tactgcctat acctactggt tcgtaaacct gttctttttc acctcgctgt
    48841 tgccgcgcgt tattgcttat gcttcgtatg ccttcctcgg ctatgaatat attatgacgc
    48901 cggttgccac caccattatc agtatggtgc tgttcgcctt ctccacctgg gtttccacca
    48961 acggggcgaa aatgttgggg ccaattacct ccgtcacttc aacgctgatg ctgctgttaa
    49021 cgctctccta cattttactg gcaggtacgg cgctggttgg cggcgtacag cctgctgacc
    49081 ccatcaccgt tgacgcgatg atcccgaact tcaactgggc gttcctcggc gttaccacct
    49141 ggatctttat ggccgcaggt ggcgcggagt ccgtcgctgt gtacgttaac gacgtcaaag
    49201 gcggttcgaa atcgttcgtt aaagtgatca tcctcgccgg gatttttatc ggtgtactat
    49261 attccgtctc ctcggtgctg attaacgtct tcgtcagcag caaagagttg aaatttactg
    49321 gcggatcggt gcaggtattc cacggcatgg cggcgtattt tggtctgccg gaagcgttga
    49381 tgaatcgctt tgtcggtctg gtgtccttta ccgcaatgtt cggttccctg ctgatgtgga
    49441 ccgcaacgcc ggtgaaaatt ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa
    49501 ccgtcgaact taacgaaaac ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg
    49561 tcatcccgct gatgattatc ccgatgctcg gttccaatac tgtgcaggat ctgatgaata
    49621 ctattattaa tatgaccgcc gcagcgtcca tgcttccgcc gttattcatc atgctggctt
    49681 acctgaattt acgcgccaaa ttagatcacc tgccacgcga tttccgtatg ggctcccgcc
    49741 gcaccggtat tatcgttgtt tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt
    49801 cgacattccc gactggcgcg aatattctga ccatcatttt ttataacgtc ggcggtattg
    49861 ttatattcct tggctttgcg tggtggaaat acagtaaata tataaaggga ttaacggctg
    49921 aagagcgcca tattgaagcg acgccagcca gcaatgttga ttaaacagac taataaaaaa
    49981 tgagcaggca ttttccctct tcctggatga ggagaggga
//
LOCUS       DAZFKY010000008        49888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFKY010000008 DAZFKY010000000
VERSION     DAZFKY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..817
                     /locus_tag="ACHKCL_000433"
     CDS             <1..817
                     /locus_tag="ACHKCL_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5761475.1"
                     /translation="IILESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLL
                     KRIMSINDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIM
                     ANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRA
                     ISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNA
                     RDSKHTGRMFSMHNCQINPSAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1007..1120
                     /gene="yhiY"
                     /locus_tag="ACHKCL_000434"
     CDS             1007..1120
                     /gene="yhiY"
                     /locus_tag="ACHKCL_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5761476.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1183..2250
                     /gene="yhiI"
                     /locus_tag="ACHKCL_000435"
     CDS             1183..2250
                     /gene="yhiI"
                     /locus_tag="ACHKCL_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5761477.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2247..4982
                     /gene="rbbA"
                     /locus_tag="ACHKCL_000436"
     CDS             2247..4982
                     /gene="rbbA"
                     /locus_tag="ACHKCL_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5761478.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4982..6106
                     /gene="yhhJ"
                     /locus_tag="ACHKCL_000437"
     CDS             4982..6106
                     /gene="yhhJ"
                     /locus_tag="ACHKCL_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5761479.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6179..6454
                     /locus_tag="ACHKCL_000438"
     CDS             6179..6454
                     /locus_tag="ACHKCL_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5761480.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6451..6810
                     /locus_tag="ACHKCL_000439"
     CDS             6451..6810
                     /locus_tag="ACHKCL_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5761481.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6860..7720)
                     /locus_tag="ACHKCL_000440"
     CDS             complement(6860..7720)
                     /locus_tag="ACHKCL_000440"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO5761482.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7752..8021)
                     /locus_tag="ACHKCL_000441"
     CDS             complement(7752..8021)
                     /locus_tag="ACHKCL_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5761483.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8011..9519)
                     /locus_tag="ACHKCL_000442"
     CDS             complement(8011..9519)
                     /locus_tag="ACHKCL_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5761484.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9512..10870)
                     /locus_tag="ACHKCL_000443"
     CDS             complement(9512..10870)
                     /locus_tag="ACHKCL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5761485.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10947..11228)
                     /locus_tag="ACHKCL_000444"
     CDS             complement(10947..11228)
                     /locus_tag="ACHKCL_000444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5761486.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(11225..11698)
                     /locus_tag="ACHKCL_000445"
     CDS             complement(11225..11698)
                     /locus_tag="ACHKCL_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5761487.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11723..12469)
                     /locus_tag="ACHKCL_000446"
     CDS             complement(11723..12469)
                     /locus_tag="ACHKCL_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5761488.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12668..13069)
                     /gene="nikR"
                     /locus_tag="ACHKCL_000447"
     CDS             complement(12668..13069)
                     /gene="nikR"
                     /locus_tag="ACHKCL_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5761489.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13075..13881)
                     /gene="nikE"
                     /locus_tag="ACHKCL_000448"
     CDS             complement(13075..13881)
                     /gene="nikE"
                     /locus_tag="ACHKCL_000448"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5761490.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(13878..14642)
                     /gene="nikD"
                     /locus_tag="ACHKCL_000449"
     CDS             complement(13878..14642)
                     /gene="nikD"
                     /locus_tag="ACHKCL_000449"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5761491.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14642..15475)
                     /gene="nikC"
                     /locus_tag="ACHKCL_000450"
     CDS             complement(14642..15475)
                     /gene="nikC"
                     /locus_tag="ACHKCL_000450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5761492.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15472..16416)
                     /gene="nikB"
                     /locus_tag="ACHKCL_000451"
     CDS             complement(15472..16416)
                     /gene="nikB"
                     /locus_tag="ACHKCL_000451"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5761493.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16416..17990)
                     /gene="nikA"
                     /locus_tag="ACHKCL_000452"
     CDS             complement(16416..17990)
                     /gene="nikA"
                     /locus_tag="ACHKCL_000452"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5761494.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18101..18688)
                     /gene="acpT"
                     /locus_tag="ACHKCL_000453"
     CDS             complement(18101..18688)
                     /gene="acpT"
                     /locus_tag="ACHKCL_000453"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5761495.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18690..19919)
                     /locus_tag="ACHKCL_000454"
     CDS             complement(18690..19919)
                     /locus_tag="ACHKCL_000454"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO5761496.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19916..20647)
                     /locus_tag="ACHKCL_000455"
     CDS             complement(19916..20647)
                     /locus_tag="ACHKCL_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO5761497.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20647..21111)
                     /locus_tag="ACHKCL_000456"
     CDS             complement(20647..21111)
                     /locus_tag="ACHKCL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO5761498.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21108..22277)
                     /locus_tag="ACHKCL_000457"
     CDS             complement(21108..22277)
                     /locus_tag="ACHKCL_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO5761499.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22279..22863)
                     /locus_tag="ACHKCL_000458"
     CDS             complement(22279..22863)
                     /locus_tag="ACHKCL_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO5761500.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22860..25178)
                     /locus_tag="ACHKCL_000459"
     CDS             complement(22860..25178)
                     /locus_tag="ACHKCL_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO5761501.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25147..25752)
                     /locus_tag="ACHKCL_000460"
     CDS             complement(25147..25752)
                     /locus_tag="ACHKCL_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO5761502.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25749..26171)
                     /locus_tag="ACHKCL_000461"
     CDS             complement(25749..26171)
                     /locus_tag="ACHKCL_000461"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO5761503.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26175..27851)
                     /locus_tag="ACHKCL_000462"
     CDS             complement(26175..27851)
                     /locus_tag="ACHKCL_000462"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5761504.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(27842..28195)
                     /locus_tag="ACHKCL_000463"
     CDS             complement(27842..28195)
                     /locus_tag="ACHKCL_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO5761505.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28182..29543)
                     /locus_tag="ACHKCL_000464"
     CDS             complement(28182..29543)
                     /locus_tag="ACHKCL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFO5761506.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29540..30121)
                     /locus_tag="ACHKCL_000465"
     CDS             complement(29540..30121)
                     /locus_tag="ACHKCL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761507.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(30126..30377)
                     /locus_tag="ACHKCL_000466"
     CDS             complement(30126..30377)
                     /locus_tag="ACHKCL_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5761508.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30389..30646)
                     /locus_tag="ACHKCL_000467"
     CDS             complement(30389..30646)
                     /locus_tag="ACHKCL_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO5761509.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30621..31442)
                     /locus_tag="ACHKCL_000468"
     CDS             complement(30621..31442)
                     /locus_tag="ACHKCL_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5761510.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(31439..32161)
                     /locus_tag="ACHKCL_000469"
     CDS             complement(31439..32161)
                     /locus_tag="ACHKCL_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO5761511.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(32202..33260)
                     /locus_tag="ACHKCL_000470"
     CDS             complement(32202..33260)
                     /locus_tag="ACHKCL_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5761512.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33329..33706)
                     /locus_tag="ACHKCL_000471"
     CDS             complement(33329..33706)
                     /locus_tag="ACHKCL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5761513.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(34098..35147)
                     /gene="yhhT"
                     /locus_tag="ACHKCL_000472"
     CDS             complement(34098..35147)
                     /gene="yhhT"
                     /locus_tag="ACHKCL_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5761514.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            35279..36496
                     /gene="yhhS"
                     /locus_tag="ACHKCL_000473"
     CDS             35279..36496
                     /gene="yhhS"
                     /locus_tag="ACHKCL_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761515.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNDIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(36500..37057)
                     /gene="dcrB"
                     /locus_tag="ACHKCL_000474"
     CDS             complement(36500..37057)
                     /gene="dcrB"
                     /locus_tag="ACHKCL_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5761516.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(37130..37795)
                     /gene="yhhQ"
                     /locus_tag="ACHKCL_000475"
     CDS             complement(37130..37795)
                     /gene="yhhQ"
                     /locus_tag="ACHKCL_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5761517.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            38016..38261
                     /gene="tusA"
                     /locus_tag="ACHKCL_000476"
     CDS             38016..38261
                     /gene="tusA"
                     /locus_tag="ACHKCL_000476"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5761518.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(38363..40561)
                     /gene="zntA"
                     /locus_tag="ACHKCL_000477"
     CDS             complement(38363..40561)
                     /gene="zntA"
                     /locus_tag="ACHKCL_000477"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5761519.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40635..41261)
                     /gene="yhhN"
                     /locus_tag="ACHKCL_000478"
     CDS             complement(40635..41261)
                     /gene="yhhN"
                     /locus_tag="ACHKCL_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5761520.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            41402..41761
                     /gene="yhhM"
                     /locus_tag="ACHKCL_000479"
     CDS             41402..41761
                     /gene="yhhM"
                     /locus_tag="ACHKCL_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5761521.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(41764..42033)
                     /gene="yhhL"
                     /locus_tag="ACHKCL_000480"
     CDS             complement(41764..42033)
                     /gene="yhhL"
                     /locus_tag="ACHKCL_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5761522.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(42023..42619)
                     /gene="rsmD"
                     /locus_tag="ACHKCL_000481"
     CDS             complement(42023..42619)
                     /gene="rsmD"
                     /locus_tag="ACHKCL_000481"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5761523.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            42769..44265
                     /gene="ftsY"
                     /locus_tag="ACHKCL_000482"
     CDS             42769..44265
                     /gene="ftsY"
                     /locus_tag="ACHKCL_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5761524.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            44268..44936
                     /gene="ftsE"
                     /locus_tag="ACHKCL_000483"
     CDS             44268..44936
                     /gene="ftsE"
                     /locus_tag="ACHKCL_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5761525.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            44929..45987
                     /gene="ftsX"
                     /locus_tag="ACHKCL_000484"
     CDS             44929..45987
                     /gene="ftsX"
                     /locus_tag="ACHKCL_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5761526.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGNGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            46232..47086
                     /gene="rpoH"
                     /locus_tag="ACHKCL_000485"
     CDS             46232..47086
                     /gene="rpoH"
                     /locus_tag="ACHKCL_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5761527.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            47357..48460
                     /gene="livJ"
                     /locus_tag="ACHKCL_000486"
     CDS             47357..48460
                     /gene="livJ"
                     /locus_tag="ACHKCL_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5761528.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            48610..48876
                     /gene="ataR"
                     /locus_tag="ACHKCL_000487"
     CDS             48610..48876
                     /gene="ataR"
                     /locus_tag="ACHKCL_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO5761529.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            48883..49410
                     /gene="ataT"
                     /locus_tag="ACHKCL_000488"
     CDS             48883..49410
                     /gene="ataT"
                     /locus_tag="ACHKCL_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO5761530.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(49407..49790)
                     /gene="panM"
                     /locus_tag="ACHKCL_000489"
     CDS             complement(49407..49790)
                     /gene="panM"
                     /locus_tag="ACHKCL_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5761531.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        11841 a        13093 c        13543 g        11411 t
ORIGIN      
        1 cattatttta gaaagtcttc agccttattt agttattaat aaagacgcag tgacgccatg
       61 tacgtttttg ggtgaccaaa ccggcgatcg atttagtaat atctgtggcg atcaatttat
      121 catcgatttg ttaaaacgca taatgagcat caatgataac gttcatgtat tagctggaaa
      181 tcatgaaacg aattgcaatg gtaactatat gcagaacttc acgcgtatga aaccacttga
      241 tgaggatacc tacgacggga taaaagatta tcctgtatgt ttctatgact ccaaatacaa
      301 aataatggca aatcatcatg ggataacttt tgatgatcag cgaaaacgtt acattatagg
      361 accgataacg gtctctattg atgaaatgac caatgctctc gatccggtcg aattagcggc
      421 aattattaat aaaaagcatc atactataat taatagtaaa aagtttaaaa ctagccgggc
      481 tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca gacctaaact
      541 tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg cacataacgg
      601 taatggtgga agagaatgca taggtgaaac ggggacagtt ttaggtctta acgcccgtga
      661 cagtaaacat acgggaagaa tgttcagcat gcataattgc caaattaacc ctagtgcggg
      721 gcctgagata accactccct ggaagtctta ccaacatgaa aaaaacagaa atggacttat
      781 gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca tgaatattaa
      841 tgttactatc tggttgagac tgttgataaa acgtaataag gaatgctctg ttcctgcaac
      901 tgaacaggcg tgaaacgcta tccaacagga tggataccgt tttgtcagcc agttttatag
      961 atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
     1021 actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg ctggtcgtat
     1081 ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg gcggcagttg
     1141 taacacgcgt cgtttgctta cagggattat ggcgggtcga ttatggataa gagtaagcgc
     1201 catctggcgt ggtggggtgt cgggttactg gcggtggctg ctatcgtagc gtggtggctg
     1261 ttgcgcccgg caggtgtgcc ggaaggcttt gccgtcagca atgggcgcat tgaagcgacg
     1321 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
     1381 cagtttgttc gcgaaggcga agtgctggcg aagatggata ctcgcgtgtt gcaggagcag
     1441 cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc cgcgcaggct
     1501 ttgctggagc aacgacaaag cgaaactcgc gccgcacagt cgctggttaa tcaacgccag
     1561 gccgaactgg actccgttgc caaacgtcat acgcgttccc gctcgctcgc ccaacgaggg
     1621 gctatttctg cgcaacagct ggatgacgat cgcgccgccg ctgagagcgc ccgagctgcg
     1681 ctggaatcag caaaagccca ggtttcggct tctaaagccg ctatagaagc ggcacgcacc
     1741 aatatcattc aggcgcaaac ccgcgtcgaa gccgcacaag ccactgaacg gcgcattgcc
     1801 gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
     1861 gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt cgatctcagc
     1921 gacgtctata tgaccttctt cctgccaacc gaacaggcgg gcacgctgaa acttggcggt
     1981 gaagcccggc tgatcctcga tgccgcgcca gatctgcgta ttcctgcaac catcagtttt
     2041 gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga acggctgaaa
     2101 ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
     2161 gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
     2221 gacgacctcg tggtgaggtt gccgcaatga cgcatctgga actggttccc gtcccgcctg
     2281 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg aacaatatca
     2341 cactcgatat tccggcccgc tgtatggtcg ggctgattgg tccggatggc gtcgggaagt
     2401 cgagtttatt gtcattgatt tccggtgctc gtgtcattga gcagggcaat gtgatcgtgc
     2461 tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc atcgcctgga
     2521 tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa aacgtcgatt
     2581 ttttcgctcg cctgttcggt cacgacaaag cggagcggga agtgcgaatc aatgagctgc
     2641 tgaccagcac cgggttagca ccgtttcgcg atcgtccggc agggaaactc tccggcggga
     2701 tgaagcaaaa actggggctg tgctgtgcgt taatccacga cccggaactg ttgattcttg
     2761 atgagcctac gacgggggtt gacccgctct cccgcgccca gttctgggat ctgatcgaca
     2821 gtattcgcca gcggcagagc aatatgagcg ttctggtcgc cacggcctat atggaagagg
     2881 cggaacgctt cgactggttg gtggcgatga atgccggaga agtgttggca actggcagcg
     2941 ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt ataaatctgt
     3001 taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccatat caacctgaaa
     3061 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
     3121 ttgatcacgt caatttccgc attccacgcg gggagatttt tggttttctt ggttcgaacg
     3181 gctgcggtaa atccaccacc atgaaaatgc ttaccggatt gctgcccgcc agcgaaggtg
     3241 aggcgtggct gttcgggcaa ccggttgatc caaaagatat cgatactcgc cgtcgggtgg
     3301 gctatatgtc gcaggcgttt tcgctctata acgagctcac cgtgcggcaa aaccttgagt
     3361 tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcaagagtg gctgaaatga
     3421 gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg ccgctcggca
     3481 ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggaaatg ttaatcctcg
     3541 atgaacctac ttctggcgtc gatccggtgg cgagggatat gttctggcag ttgatggtcg
     3601 atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg aacgaagcgg
     3661 aacgctgcga ccgtatctct ctgatgcacg ccggaaaagt gctcgccagc ggtacaccgc
     3721 aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc gcctatttgc
     3781 aggaagcggc aggacagagc aacgaagccg aagcgcctcc cgtgatacac gacaccacac
     3841 acgcgccgcg ccagggattt agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
     3901 tggaactgcg acgcgatccg gtacgttcga cgctggcgct gatgggaaca gtgatcctga
     3961 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgcttg
     4021 accgcgacca gaccgtcagt agccaggcgt ggacgctcaa tctctccggt tcccgttact
     4081 ttatcgaaca gccgctgctt accagttatg acgagcttga tcgtcggatg cgtgcgggcg
     4141 atatcacggt ggcaattgag atcccgccca atttcgggcg cgatatcgcg cgtggtacgc
     4201 ctgtggaact cggtgtctgg atcgacggtg cgatgccgag ccgcgccgaa actgtaaaag
     4261 gttacgtgca ggccatgcac cagagctggc tacaggatgt ggcgagccga caatccacac
     4321 cctccagcca aagcgggctg atgaatattg agacgcgtta tcgctataac ccggacgtaa
     4381 aaagcctgcc agcgattgtt ccggcggtga tcccgcttct gctgatgatg atcccgtcaa
     4441 tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc aacctttacg
     4501 tgacccccac cacccgtagc gaatttttgc ttggcaaaca gctgccgtac atcgcgctgg
     4561 ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc gtaccgcata
     4621 aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatc gccaccggaa
     4681 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc ggtacggcga
     4741 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
     4801 aagggccggg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
     4861 gcgggacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt atcccgttgc
     4921 tgatagccat accgctggtg atgggcttaa gcatcctgct gctgaaaaaa caggagggat
     4981 gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca gtctactcgg
     5041 tgataaagcg atgctgacat tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
     5101 gaccgtcacg ccaggatcgt tgaacctggc accgatcgcc attgccgata tggatcaatc
     5161 gcagttatcg aaccggatcg ttaacagctt ctatcgcccg tggtttttgc caccggagat
     5221 gatcaccgcc gatgagatgg atgccggact ggacgccggg cgttatacct ttgcgataaa
     5281 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccagata ttcaggtgaa
     5341 cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
     5401 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
     5461 gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg gcggggtgat
     5521 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
     5581 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
     5641 catgatggcg aagatctggt cgatggggtt ggtggtgctg gtggtatcgg gattatcact
     5701 ggtgctgatg gtaaaaggcg tgttgggcgt accgattgaa ggctcgatcc cgctgtttat
     5761 gctgggcgtg gcgcttagtc tgtttgccac cacgtcaatc ggcattttta tggggacaat
     5821 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
     5881 gctttccggt ggttccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
     5941 gatcatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc gtggtgccgg
     6001 attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg cgtttttcac
     6061 cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa gggaaacttg
     6121 acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag gacatgccat
     6181 gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc aaatatttaa
     6241 aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta aagcattagg
     6301 cggagaaatt aaggaaggaa gaggttcgcg ttgtaagctc atactaaata tgagcgttgc
     6361 gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag aaagcgtgcg
     6421 tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc aaactctatg
     6481 gagattgccg ggcaacctgc tgttatcact tacgtgccag aactgaatgc atttcgtggc
     6541 aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca ggggctgcaa
     6601 aaagaagggg aactttccct gcgagagtat cttgaggatt gtaaagctgc gggtattgag
     6661 ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga atcgctgagt
     6721 gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta tattattgaa
     6781 acactgaatg agcgtttgaa tcatttataa cttatagcgg cccaacaatt tctgttgcgc
     6841 cgctatattt ttgtggctct taagatgcct ttctaaccaa ccgatgcccc tcattcatca
     6901 tcaggatttt gctatacaca tcttcctcga cggcgttttt tgctgatgcc atgactcgtg
     6961 ccagattcgc ctcgttgtga ttattcacat acgctttgcc gacggcggtg ataaacgctt
     7021 tgcgcagatc gctggcgata tttactttag cgacgcgata attaacaaag ctgcgtaata
     7081 tctccggggc gataccggag ccgccgtgaa taaccaacgg caccggacag acttcggcaa
     7141 tgcgcttcag cagggggata tcgatacgcg gtatatcgtc caggccatga acgttgccaa
     7201 tagaaactgc cagcatgtcg caaccggtac gctcgacaaa ggtcttcacc ttttccggtt
     7261 ccgttttgca atcggcttcg ctgacgtgat catcttcttt gccgagaatc gcccccagct
     7321 ccgcttcaac cggtacgcca taggatttac agaaatcgac agcttcctgg gtgaaagcga
     7381 tattctcttc aaaagagaat gcggcaccgt caatcatgac cgaggtaaag cccgcgcgca
     7441 ccgcctgttt aacatcctcc tgggttttcc cgtgatccag atgaagactg accggaacat
     7501 ccattttatc ggcgtgacgt ttcacgattt cataaatgta atcgtatccc gagagttgtg
     7561 cattggttgg cgcaatctga atgaagttgg gtaatcccga acgctcaata gcgtttaaaa
     7621 ttgaaattgt cgtctccaga ttggtggtat taaatgcgcc cgctaatacc cgtttttcct
     7681 gaatgcggtc gagcagtatc cgtccattaa ctaatggcat attgaaaacc tcatctttta
     7741 atatcagtta attattcagc ttcaccgaag cggtcgcgaa ataagtcggt gagagtctgt
     7801 atggcctcgt tttcatcatc accgtcgcag cgtatttcca ccgtttttcc gccttttatt
     7861 ccggcgcgca taatattcat catgctggtg gcattaatat cattaccctc atagtgcagc
     7921 gtcactttac agctatattt tttggcggct ttcgccagac aggccgccgg acgtgcatgc
     7981 aggccagtac tgtttctgac ctccacggtt ttattcaaca tagtgttcct ctcgatgatg
     8041 taatggcatc acgccttctt gttgtaattt caactgatac cagcttttcc atttgtgctg
     8101 ataacgctgt aattgatggt atctcgcccg attcggtaac aaacgttggg ctatagcgac
     8161 tggcggcggt ggaatatccg gtgcggcggt taataatgct ccgacagaaa ccgcctcgtt
     8221 aaatgcaatt gcttcaaccg gacgctgtaa aatatccgcc tttaattgca gccaaagagg
     8281 attatgggtt gccggaccga tcacttttaa cgcgctggtg ccaggcacgt taaaacaggc
     8341 gaagcagttg gcaaattcca tggttaatcc cagtgatacg ctggcaatta acatgtcgat
     8401 acttaacgta tcgccaagcc cataaaataa tccacgggta tggcgatttt tatagggcga
     8461 acccgaaccg cgaagatgtg gaataaagac gggaatgtca tcgagcaacc aattccccgc
     8521 cagataatca gcatgtccgc gagtaagtga tgcggcgatc tcctcatcgg ttagccggaa
     8581 cgtgttgcgc agccactcca gcgcgaaacc gcccacgggc agcgacgcaa acagggtgaa
     8641 ctggttggca agtgagtaac agccgtttgc cagcttgtca cgtttggcct gttcatccag
     8701 cgtcggttgt gtatccagtt gcagaatgcc ttccgtggtc cccgtcgagt taaggatatc
     8761 gcctggcatc atctgcaacg ctcgcgcacc caccatatgg tcatgtccgg ccagcgtcac
     8821 gctgaccggt tgcgagaacc caagcgtctt gcaaagtgac tcgctgaccc atccggcgtg
     8881 ctcgcctggc tgcaccaatg gcgcaaatgc cgtacaggga acgtgtaaca acgccgccgc
     8941 tttcgctgac cattcgcctt tgcgcacgtc cagacacagc gtgcggctgg ctaaggtgat
     9001 atccgtgtgc tgttcgccag tcagccgcca gagcagcact tccggcgcat gtagccagcg
     9061 caggccgcgg cgattacgca gcgggtaatg ttccagcaac cagcgcattt tgaaggcaga
     9121 gtaattgctg tgtagtggta gaccgcagat gtcataaagt gccgcactgt ctgcctcgct
     9181 aagcgttgcc agatactctt caccgcgacg gtcataccat gccagcattg gcgtcaggat
     9241 ctcgccatgc tcgtcaagga acacgcctga ttcgccaaaa ctggcaatgc tgatccgtct
     9301 gactggcagc ggcgaagcgg cgttcagttg cgctatcgcc tggcggacct cctgccagag
     9361 ggcgtcgata tcgaaatcga catcgccctg tggggagatc tgctttgccg tcacgaattt
     9421 atgcgcgccc aacgtcgttg cgtccaggca ggaaaagcag gtgactttgc aattggtggt
     9481 gccaatatcg ataacgatag ctgcgctgtt atcaggcata aaccgtgctc cgcgtcattc
     9541 ggaaaatgcc atacagtacc agggcgataa ccggcaccag accaatggcg atgagattgc
     9601 cgttgaagat ttccagcagc cagaagcgga acgggttacc gccatcaaga aaactggaaa
     9661 cctggccgct ggagccttcg gcaaatttga agttaacgtc tttcgccatt ccggtaataa
     9721 acggcgcgat tttggtagca atccataggt cggcaataat caccgggatc gccgccagaa
     9781 cggtgcggaa aatattgcca cggctggcta aagcaatcat cgacgccatt accgccaggt
     9841 tggcaaggtc gccaagcggc aggacttcat tacccggaag gacaaacgcg ataaagaccg
     9901 agattggcgt tagcagcagg gcggtggaga taatcgccgg attacctacg gcaacggcga
     9961 tatccaggcc gatatagaga tcgtcacggt cagggtattt ggcattgaga tactttttaa
    10021 tagcttcaga cagcggtaat aaaccttcca tcaggatgcg taccatgcgt ggcaggatga
    10081 acatcacacc gccaatgctg atgccaagca gcaatacttt tttgaaatcg tatccggcaa
    10141 ttacgccgag cagaatgccc agaatagtgc caaccatcat cggttcgccg aagatgccaa
    10201 actttttctg tacggtttcc gggtcgatat gaatacgatt gaggccaggg atatggtcga
    10261 tgattttgtc gaccagcaga ccgaccggga agaacaccac cgaagagagc gtcggcaatg
    10321 agatcccttc cagtccaaag tatttttgta ccagtggcgc agaccagtcg gcgagtttaa
    10381 gtactaccac cgcagcaatc gccgccgcca gcaaaccaaa gaacaggctg ccggtgctgt
    10441 aataaaccaa cgtaccagca agggcaaaat gccagtagtt ccacatatct acgtcaacgg
    10501 tacgggtttt atttaacgcc agcatcgcca cgttaatcag aatggtcagg ggaataacaa
    10561 acggggcaat tggcgaaccc catgtaatag cggaaagcgg cggccagcct aaatctacca
    10621 ccggtaaatt aattccggtg cgttcaacca tcgcctgggc cgctggcccg acattgctgg
    10681 tcagaatatc gaaaatggca taaataccga caaaaccaat gccgactgtt aatgacgaac
    10741 gtaaggcttt agcgggtttt actttaaaac agagagcaag aataaataac accaacggca
    10801 acattaccgt cgggccaaat cccaatatat attgcacaat attatagagg gtatgcgcga
    10861 tatcgttcat aataaacctc tttttcaggc gagagcgcac ctcgcgttag cgcctgatgg
    10921 agtgggttga aattaattat tattaattac tttttaagga tagaaacaat ttgcgccagt
    10981 actttttctt cgccaatacc ggtaataatc ggcaagccgt tcaccaccgg gacgtcgagt
    11041 tcataaggca ctttagttgt tgaaactacc agtgctacgc cgtcagtatt tccaggtaaa
    11101 gaggcaacgt tggtttggga ataattgacg ttaagacctt gctctttgca atattccatg
    11161 actttttctg ttaccgccgt ggatgtggca atacccgtgg cgcagacaaa taaaatggtt
    11221 tgactcatat ttcacctcgt tctttcattt tgtttaagat tactgtgtgt atttctgttg
    11281 tttccatcgc aagcagggct tgcataaagg gcgtatcctg aatcatttcc ataatccagc
    11341 ccagggcatt taattgttta ttgctttcgc ttaaggcgag caaaaaaatc accctgacag
    11401 gtacggggtc aggatcgccg cccatatcgg caaactctac cggttccggc aggatgccga
    11461 cggcaattgc attgtgcctg acatgtttat ggtccgtgtg cggaatagcg acgcacggtg
    11521 gttcggtagg cagccccgtt gggtacgtcg cctcacgttt cagtacgtga tcggcaaaat
    11581 cagggacgac cagctcggcg gcaaccaacg gttgcgctaa ctggcggatg atctgttcaa
    11641 cgcttaaccc tgccggaagt cgttgaatat aattttcctg aaaatgtatg ccttgcattt
    11701 tattcctctt aaaaaagtgt acttaactca cttcccgccg ttgcagtacg gtgccaagat
    11761 aatatttatt tccttttaac cagacgttgc caaactcaat gggtaatcct cgggagaaaa
    11821 agactaactg ctccagatgc aaaacgggtg cgtcttcact gatatctaaa aaatgaccac
    11881 gttcattacc aattaatcgc gcggcatagc gactttccga gtaacgaatt tttctttgcg
    11941 acaaactttc tattgttgga aataaattgt gttgattaaa atcgatttca gcaataccgg
    12001 ggcatagctc aatattgata cggttctcta tcagcatcgc tttttcatca ccaattgagc
    12061 gtaagcgttc aaggtaaaga atatcctgac cgggcgttat tcttaatttc tctgccacat
    12121 aacgattagc cgattcaatc cgtgacgtaa taacttcagt ggtaaaatgt attttctggc
    12181 tttccagcga ttccgcgaat gacaataatc cttcacctaa cggatatgcc acgttctctt
    12241 ttttgacaaa tgttccctta ccctggacct gtattaacac gccttctttt accagttggg
    12301 cgaccgcttt tttaacggtt ccccgactca cgtccagcat atccataatc tggtattccg
    12361 aaggaatgcg atcgtcttcc accagatctc cggtataaat actttctctt atccactcca
    12421 ccacttgttg atagagcggt atttgtgacg acttacttaa tgatttcatg gcgcgcacct
    12481 gaacatttac agttgtctat ggacaatcat ggacaacttc aagcgtatag taggtgaacg
    12541 ggagaaaaag aggatcgaag cgtctcacat tttgagggac caaatcgtga tcaaaaaaat
    12601 gtgacgtgag tcgatatttg tcagaagttt actgcgccgt aaggaaataa cggcgcgttg
    12661 gtaggtgtta atcttccttc ggcaggcact gcaaatgccc gtgccgcacg ccgcgctggg
    12721 cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac acggcgattt
    12781 ccagacagtc gtcgtggttg atgtgcacat gtagcgtggc gacggagagg tcgtggtgat
    12841 gatgctgggt ggaaacaata cggctggcta agtcgcgttt ttcgtgttca tacacatacg
    12901 acagcaccgc gaaaccttgt gtgccgtgct gctgggtagc ttcctgcgcc agggcgctac
    12961 gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg ctcaggctgt
    13021 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac
    13081 cttttctgtg gcgcgacggc gcacggggaa tgcgggtaat atcgcgtttt gtagcacacg
    13141 tccggcgtca gaggaaaagg ttaatttctc tcccaccgcc tgggtttcga cgatttgtcc
    13201 gttgtccatc accatcactc gctggcaaaa gcgttccacc aggcgtaagt catgagtgat
    13261 gaacaggcag gcagtgccaa actgttgttg tagctttttc agcaggcgaa tgacgcccgc
    13321 ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagcaatt tcggttcgac
    13381 cgccagcgcg cgtgccaggc agacgcgctg gagctggccg ccgcttaact gcggtgggcg
    13441 cttgtcgagc acgctgtcgt cgagatcgac cgcgtgcagc atttcgctgg cacgcgccag
    13501 ttgttcggat tttttcagcg atagcaggtg gcgcatcggt tcccgcagga tctcgcgcac
    13561 ggttttgcgc ggattcacgg cgctgatgga gtcctgaaat accatctgaa tatcgcggcg
    13621 gaacgcttta cgctgggcgc gattgagctt tgccaacgat tcgccacgcc agctaatatt
    13681 tccctgtgag ggcgattcta agcctaccag tagccgcgag agagtgcttt ttccgcagcc
    13741 gctgcgtccc agcagggcga cggtttcacc gcttttcagg gtcagggaaa cgttattcag
    13801 caccgcctgg tgctgatgtt ttccgctaaa cccaccgtgg gcatagtgat gggaaaggtc
    13861 ggagacgtta agtaaagtca tgatgccagc tccataccgt agagggcgag atgagcggaa
    13921 accaggctgc gcgttaccgc atgtttgggg gcgttaaaca gcgtttcgac atcgccctgt
    13981 tcgacaatct taccgtcaga cattaccgct acgtcatccg ccagacgcgc caccacgccc
    14041 atatcatggg tgaccagcag catccccggc gcctgttttt gcataatgct ttccagcaga
    14101 tcgagaatgc gtgcctgtgc taccacgtcg aggtcggtgg ttggttcatc ggcgatgata
    14161 aacggtgatt cgcaaagcac cgccatcgca atcatcatgc gctgcaacat gccgccgctc
    14221 atctcgaacg ggtacagctt cagcacgcgt gcggcgtttt ccagccccac cgcttctatg
    14281 gcagcggtaa gcgtagcgtc atcggcgggt ttccccagcg ccaggcacgt ctcccgcgcg
    14341 tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat gatggtggca
    14401 attttgatgc cgcgcagggc gcagggagaa accggtttgc catcggctaa aatttccccc
    14461 gccgtctggc gaacgcctgc gggcagaatt cccagcgtcg cggcgcaggt taatgatttc
    14521 ccgctgccgc taccgccgac taacgccagc acgcgcccgc gtttcagggt taacgacacg
    14581 ccgtgcacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat ctgttgcggc
    14641 attagtgtgc gtgctccgtc accagatgag gatccagatg atcgcgcagt gcgtcaccca
    14701 ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac atttgcagcg
    14761 gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg
    14821 cggtcacgcc gaggccgagg aaggacatcc cggcaacgtg cagcatcata tggccgatat
    14881 ccagcgttgc cagcaccagc agcgaaggga tcaccgcacc tgccagatga tcaacaaaca
    14941 ctcgcacatg gcccgcgcca gaaagccgtg atgccagcac aaactcgcgc tggcgcagtg
    15001 aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg gcgatggcga
    15061 taattacgtt ggtcagccca gtgccgagca cgccaaccat aaagaatgac agaatcgagg
    15121 tcgggaaggt cataaacata tcggcgacgc gcatggtagc ctgatcaacg cgcccgccaa
    15181 tcagcccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc aggcaggcca
    15241 ttaccgaacc gagcgacaca cgggtcgctg ccatcagccg cgagaaaata tcgcgcccta
    15301 agtgatcggt gcccaaccag tgctgcgcat ccggcgaaag caggcgcgac ggcaaatcaa
    15361 tcgcctgggg gtcatacggc agccaccact ggctggtgag cgcaatcagc gccagcaggg
    15421 cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag ttcacgcgtg
    15481 cgctccttca tgacgacgaa tgcgtgggtc cagtgcggcg ttgagcaaat cgacaatcaa
    15541 attacagacc acaaaaacca ccaccatcat cagcgtaaag cactggatca ccggatagtc
    15601 acggttaaaa atcgccgaca ccgcatagcg cccgacgccg ggccaggtaa agatgttttc
    15661 gataatcatc gtcccgccaa tcagttcgcc aatgtgcatc cccacggcgg tgatcatcgg
    15721 cagcgaggca ttgcgcagaa tatgacgacg ctcggtctgt ttgtcattca ggccgcgtag
    15781 ccgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc gcagcagccg
    15841 tgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga tgtgctgcca
    15901 gccgccgtag cccatcgcgg gtagccattg cagatacacc gaaaacgcca tcaccagtaa
    15961 aaatgccagc cagaagttgg gcatcgacac gccgagaaac gcgatgaaac gtacggcgaa
    16021 atccggcaga cggtcacgat ggcgcgccgc ccagataccg agcggtacgg aagtgagcag
    16081 aattaatacc agcgccgcac ccgccagttc cagcgtggcg ggcaggaagt tcagcatatc
    16141 gtccagcacc gggcgctgcg tggcaaatga gatgccgaaa tcaagatgca gcgccttcca
    16201 caaccaggtg ccgtactgca catacagcgg ctgatccagt cccagcatgg tgcgggtaga
    16261 ggccagcatc tccggcgtcg gcggcaggtt agacagacgc aaataatcga gcgccgggtc
    16321 gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca ccatcgggat
    16381 cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg tttaatctgt
    16441 tcgaacggaa tttcggtggc gatcggcgcg taggggatgt tacccagctc cggttttgat
    16501 accaccatca ttgagatgta actgataggc agataaaccg cctcgtcatg cagacgggtc
    16561 agaatgtcgc gatacagtgc ctgacgttgt gcttcgtcat gggtcgccag cacttcgccg
    16621 atctctttat caatcagcgg cttgtcggct aatccttgct gtgcctggaa gtcagcgtgc
    16681 gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc ccaggtgcgg
    16741 tggaaaatca tgccaaaacg accgtcgcgc tgacgcgcat agatactgct ctcttcttcg
    16801 ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat ttccgccatc
    16861 gatttgctta acgcatcggt gccgatgaac gaaagttcaa tgtgcagcgg ctgaccattt
    16921 ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag caacgctttc
    16981 gctttctgcg gatcgtactg gcgcggtttc aggccgaggt tggcgtaggg cacagaaggg
    17041 gcaaacaggg tgtcggcgac ctgctgggtg ccatacaacg cgttatcaat cagcgatttt
    17101 ttgtttaccg cgtaattaag agcttcacgt actgccagct cattggtggg ggctttggcg
    17161 gtattaagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtatg gtaagctggg
    17221 ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc gtacagcagg
    17281 tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtctgggat gacgttaaaa
    17341 gtgatcttct taattgccgg cttttcgccc cagtagtttt cgttacggac gaagacatcg
    17401 tactgattca gtttcgattc ctgcaaaacc cacggtccgg tgccaatcgg cgctttaatc
    17461 ccgttcatgg tttcatggtt tttaaactgc gagggggcga taaagcggaa agggcgcggc
    17521 agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtgatttg cagctctgtt
    17581 ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc gtgacgttga
    17641 cggttatcga gcactgcgcg gaagttttct gccgccgcct ctgcatcaaa cggttcgccg
    17701 ttagagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc ttctgaatga
    17761 gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt caccaatggt
    17821 tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg gtttagtggc
    17881 ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg gacgataaaa
    17941 gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat ggtaacccca
    18001 atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt catactgatt
    18061 acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc
    18121 attgcacact gtcggcggtg agcgtaaagg gagtaggggt gcagatcgcc aggcttaaat
    18181 tttctagctg acaatggctg actgacagcg aggagtgata ggtgctgtct acgctgacga
    18241 tttgccaggc gctgccacca cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc
    18301 agaacatttc cagttgctga tcaggatgca cggcgtccat ctcagcatgt tccccgaggc
    18361 tgaacacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc
    18421 agccgacttc gccttcatca ctcaacagca gggcgatatc gtcaccgcta tggcttaagt
    18481 tgaaccatag cggcgtttcc ggcgcaaatg caggtttgcc ttgttcgcca tagatgatct
    18541 ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag cgttcgcgtc
    18601 gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg cttaaggtcg
    18661 aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgat gatggatgta
    18721 ttaatgccgc caaaggcaaa gttgttactc tgcaaaaact cacaatcaac cttgcgggct
    18781 tcatgcatga tgtaatccag cgcgccacaa ttcgggtctg gtttattcag gtttagcgta
    18841 ggcgcaaacc aaccttcgcg catcatttgc agactcatcc aggcttcgag cgcgccacag
    18901 gcaccaagcg tatgaccaaa ataacttttc agcgaggaga gcggcacatt atcaccgtaa
    18961 atggttgctg tcgccagact ttctgccata tcaccgcgat cggtcgccgt accatgcgct
    19021 gaaatatagc cgatatcttg tgcgcttaac ccggcgattt tcagcgattg ctccatacaa
    19081 tactgcatgg tttcgcgctg cggctgggta atatgtgccg cgtcgcagtt agtcgcaaag
    19141 ccgacgattt caccgtaaat ggtcgcgccg cgcgctttgg cgtgctccag ttcttcgaga
    19201 atgagcgtac ctgcgccttc accaatcacc agcccgtcgc ggttttcatc gaacggtgaa
    19261 ggtgtggttt tcggcgcatc attgtgctgg ctggtggcga aaagcgtatc aaataccgct
    19321 gcttctgaag gacataactc ttcggcacca ccagcaacca tcaccgtttg ataaccgtgg
    19381 cgaatggctt cccacgcata accgattgcc tggctgccgg aagtacaggc gctggaggtt
    19441 gggatcaccc gcccgcgcag gccgaaaaag aggccagtat taacggcggt ggtgtgcggc
    19501 atcatctgca cataggtggt gccagtgatg ttattggtgt gcttttccgt cagcatggtg
    19561 gcgaactcgc ttaccgggcc ggtactgccg gtcgatgaac cgtacgcaat gcccgtttcg
    19621 ccgctggtta atatcggatc gccaatcaac cctgcctgct ctaacgccag ctcgctggca
    19681 cgggtcgaca tttgcgacac gcggcccatc gcacggatac gcttgcgggt gtagtgctcc
    19741 ggtagcgtga aatcatcaac aggcgcgcca agcagggtgt gcagcccatc gtagacctgc
    19801 cactccggca ttttgcgcac cgcattttca tatgccagta agcgtgcaga aacgtcctgc
    19861 cagttttcac caaaggcagt cacgccgccc attcctgtaa tcactacgcg acgtgtcata
    19921 acatccctcc gttgatggaa ataacctggc gggtgacgta acccgcaata tcagacatta
    19981 aatagctggc aagcccggca acttcttccg cctggcccat gcgtttcatg gggatcatcg
    20041 acattgcctc tttcagggcg ctctcttcca tttcgatcat cccggtatca ataagccccg
    20101 gcgcgatgca gttgacggtg attttgcgct tcgcgagttc gatagccagc gcttttgttg
    20161 cgccgataat tccggctttg gcagcgctgt aattcacctg gccgcgattg cccattacgc
    20221 cggaaaccga ggatagcgtg atgatgcgcc caccctggcg tgcgccgatc atcggcataa
    20281 tgcacggctg aatgacgttg tagaaactgt cgaggttggt gtggatcacc gcatcccagt
    20341 cgtcatcgct taaagccgga aaagcggcat cgcgggcgat cccggcgtta ctgaccacgc
    20401 cgtaccaggc accgtgttgc gcgatttcgt gctccagcac ttcccgacac tgttcgcggt
    20461 ttgcgacgtc gaaactgagc aaacgcccgt tacccccatt ggcgacaatg gcattcagcg
    20521 tttcctgagc gcctgtggcg tcacggtgat agtgaacgcc aatgttaaag ccgtctgccg
    20581 ccagttggca ggcgatggcg cgaccaatgc ctttgctggc accagtaacc agaactgaac
    20641 gactcatcag gacgctcctt gttgaaacag ggtggtaagt tcttccgcgg tcggctggaa
    20701 ggtgttaatg cggcccgtcg cctcgccgtc gttgatgctg cactcaaaac tgccgaagcg
    20761 atcgtcttgc atcagcagtt tgacggtaat ggtcagtgtc tggcctgcgg gcagaatgcc
    20821 agtggtgcaa agcaattcac gcgcgcccag tatcatccct aactcaatac tgtttttgcc
    20881 ctgctggtgg cgatgccagc cagaccagac gccgacggtt tgcgccatca gttcaagggt
    20941 aaaccagccg gggagattgc cgtccgggtc gagaaacggt gccagtacgc cactgggcga
    21001 aaccgtcacg cggcacaccg cgctgtcatc gctgacgctc accacgtctt ccagcagtaa
    21061 catgggcgcg tcgtggggca gataagcgcc gggggataaa tagtggctca cgaaaccctc
    21121 ccgagcagaa tgctggcgtt attgccgcca aaggcgaagg aattggacag gatcaccgga
    21181 cgtgccagcg gctgcggttt ttcgataatg ccacacggtg gcagcgtggg atcgcgcggc
    21241 gacaggctga agtcctgagc gggcagcggt aaatcacgct gtaaaatcaa catactaatt
    21301 gccgcctcgg taatgcccgc tgtgcccagc gtgtgtccgg tcaaatgttt ggtggagctg
    21361 cacggtacgc gttcgccaaa cagggcgtta accaccattg attcaatctg atcgttgagt
    21421 tgtgtggcgg tgccgtgcag gttgatatag ccgacgtcat ctggcgtaag ctgcgcatcg
    21481 gtcagcgcct gattaatagc gcggatcgct ccttcgccct gcggatgcgg ggctgaaata
    21541 tggtaagcgt cgctcgattc accaacgccc agcaacgcga tgggctgcgg ttcccgggtc
    21601 agcagcatta gccccgcccc ttcaccaatg gtgatacctg cgcggtcacg accaaacggc
    21661 tgacataagg ttggcgacag cgattcgagg ctgtgaaaac cgttaatcgg catccggctc
    21721 agggtgtctg cgccacccac aatggcggca tcgaccagcc ccgcttcgat aagcctgcgc
    21781 ccgctgatta tcgctctggc gctggaagag caggcggttg aaagggtata ggccgggccg
    21841 tcgagcgcca gccagtggct gagaaaacgc gacggatcgc cgagttcctg ttgcgcgtag
    21901 tgccatgtgg tgctcggctg cccgtgggtg cgcaggttaa cgtgcgtgtc gccttcatgc
    21961 aggccggagg tgctggtacc aagcacaatc gcaatgcgct cgcggccata tttcgcaatg
    22021 gcgtcatcaa cctgcggctg gatctgcgcc agcgccgcca gcaggatctg attgttgcgc
    22081 gagcggtgcg cagcgaattt ttccgggata agcggaagct cgccatccac gccagccagg
    22141 accgcctgcg gatgcccctg caaccagcct gcgcgcgggc gcatgccggg agcaacaccg
    22201 cgagtcaggt tggttgcgat ttcgtcaagg ttatttccca gggcgttgat catgccgacg
    22261 gcggaaatat aaatcatatc agtcacctaa gtattgaatg gtgatgtggt atttaaagac
    22321 atgctgctca atgctgattg gcacgcgttt gcccttgcga ttcagatagg tgatttccgt
    22381 gaccagtttg ccgctggcgt tacgcagctc gcgtttgtca ccgttgtcgc gaagtgtcca
    22441 tcctgcggga agttgcggtt gccaggcgct aatcggccag tggctgagca tcacgtcagc
    22501 cagtacctga cttgcgggcg gtaactgtgg gacgacgatg gattgctcgg cgcgtagccc
    22561 ttgcgcatcg taggtcacca gaaacaggcg aatgccgacc gacgacagcc cggcaagggt
    22621 gattttctga tcgtcggcgt taagcatcac taacagcgac tgggttttgc cgttgaagct
    22681 gccggtcaac agttgctgcg aattgactgc gggtgaaatc cctggtgccg gcagcgtgat
    22741 gcgcgtgcca ggttgcagcc acgcctgcgg gcggccttcc tgttctggtt gcgagtggct
    22801 acagccggtg aggataagcg tagcggtcag ggcgaacgct cgccagaatg tggatttgat
    22861 catttttttg ttctcttttt atcgggcata gccagcggcg aaagcaggaa ggcggtgaaa
    22921 ataccgctca ccagcacaat gccaaaactg ctgatggcct gggtggcgct gaataccagc
    22981 atacccagcg tcagcaaggt ggtgagcatt gccagcgcga tcgccagtag cgaagttaac
    23041 ggtgtaccgc gcggattact gaaaaacagc gtgtagttga taccgatgcc aagcaccagc
    23101 accagcgcca gcagcgaaaa gagattcacc gcctgcccgc tcatcgccag caccgccaga
    23161 ccacagccca gcgaaagcac cgaaggcacc aggctgataa gccctttgcg ccagccgaga
    23221 cgggccactg cgccgcaggc aatcactgcc agcgccacca gcaacaagcc ggttaagacg
    23281 tagcggtaaa gtgcgaacaa ttcatcaaag gtgcttttgc gatcaaccca ggcaatgccg
    23341 caagggtaat atgtggcgat ttcctgcatc aacgcgctac ttttaacccc ttcaaccggc
    23401 accagtacgc cgctttcgcc gttttccagc gtcagccaca gcagacgcca gccttcactg
    23461 gcggggcttg ccagccattc atcaacgttc actggcatgg cgttgagatc cgggttcact
    23521 gccgtcagcc cggcattttg cagcgctttt gttactgtcg gggccgccgt tttcagcagt
    23581 tgtaaatctt cctcctgccg cgcaagggag ttcagcggaa tggtgcggta gttgctgata
    23641 agcccctctt ttttcgcata ctcaagtgag gcggtatatt tctccagtcg ccgcaatgtt
    23701 tgctgtggcg aatcgccgta aaccacaaac catttttgat cgacgctctg cccggtcagg
    23761 gcggtaatgg ctttttcctg cgccagaata tgctgcggta gcgcctgtaa ctgcgagata
    23821 tcgtcatcga cgcgtagcat tgacatcccc gccagcgaaa acagcgccag cgcgacgggc
    23881 agacccagcg acagtttttt attacgccgc cacgctgcca gccagcgtag catcagcgcc
    23941 atcgccggaa ccggacgcac cggcaggcca cggcacagcc acggatgcca gaacaggacg
    24001 gtcagacagg aggcgctcaa cccgacggcg gcaaaaatcg ccatctggcg aatgccgggg
    24061 aagggggcga gcatcataat cagatacgcc gccacggtgg tgagcagcgc cagtagcaag
    24121 gcattgcgta ctttcgccag gctttgccac ggcgaaacgt cgttgccgtg aaccatccgc
    24181 tcggtgagat aatagagcgt gtagtcagcg gaaatgccga taacgctcat gctcatcacc
    24241 agcgtcatca ggtgtaattc accgaaaatc aataaagtgg cgaccgttcc cgccagcgcg
    24301 ccgatgccga tggaaatcac gcaaagcagc aacgggcgta aagagcggaa caccgccaca
    24361 atcagcaata tcacccccag cagcgtagcc acgcccaggg tggagatatc ctgcttcgcc
    24421 tgttggctgg cgtaatcgct gtaaaacacc gtgccgcgcg agagcaactg tgcctgcggg
    24481 taacgcgttt tcagatcctt ttccagcgta ttcagggtcg tgatcagctg gtgggtttgc
    24541 tgcatatcaa acgacgatcc cgccagttcg ccgtgcagca gataccagta gttgccctgg
    24601 ggatcctgcg tcaccagcca accgtccatc agccgcaaac gctggccgtt tttcgccatt
    24661 gccagctgcg agccgcgcat taacatcagc ggatcgtttt gcagctcctt gccgcttacg
    24721 ccggagaatg cggaataaag ctgggagagg atccactgtg cctgcgcttc gccgccgttt
    24781 tgcaggcggg cgcgggtgtt ggggtcaatc aggccgttgc gatgctgcca gaaaaacgct
    24841 ccccacgcct gctggctggc ggcatccatt ggccctttaa cgtcgccgag cgcagcggat
    24901 ttttgcagca gcgtcagcca ctcctgagcg accaaaggat tagcctcttt accggggctg
    24961 accagccaca ccagttggcg gtcaagacgc tgcataaagc cgtcattcag cgccggagga
    25021 atatcgccca tcgtctgttt gggtaacata gccagcacgc tactgttcag ccgtgattgc
    25081 ggcagcagga tcagcaacgc caccgccatg accaggcaga ctagccccca taacagcgcg
    25141 gggcgtttac tgggcggcaa aacgttggcg ttcgtcatcg gtcagttgcg ctggcgtcag
    25201 ttgatgttgg gtaagagcaa tatcggtgcg atcgccctgt ttatcattaa gttgaatgct
    25261 ctccagatag gttttcccgg cgagatcgat ggtgttgaaa attttatcca gcggcgtggt
    25321 ggtcggcgtc aggcgcagcg tccagcggcc ttcgcctttg tcagcaaatt cgacgcggaa
    25381 gttttgttcc agcactttgc gatcggcctg gaacaacgcg cgcagcaggt ggttaaactg
    25441 gaacatctgc gggttgtttt ctgccgtgat gatttgcggc ggctgaccgt tgatcacctg
    25501 caccatgcgt ttatcgtcca gtaatagttg catggggaac ggtgaggttt gatcccacaa
    25561 cagcccctgg tcgcgggcga tcaacatctg gccctgagat cgcagcggct gcggcagatc
    25621 tttaatcgtc cgggtttgat cgaaatgggc gcggatcacc ggttgttcgg taaagcgttg
    25681 ctgaagatcg tccagggtca gggcgctcac aaacgggcta atcagcagcg ccagcagcgg
    25741 taaaaatttc atggtttgac tcccatgcgt tcaaacagaa tatcggggct gacaaagcac
    25801 agttcgcgac tttgctcgtc gaccgcgacc tgaatggtgt atccggtggt ggcgcgtttg
    25861 cccgtttcgg catcgaaaat ctgatagcca atccgcagac ggttttcgaa ctcttcgata
    25921 tgcgcacgca cgcggatgcg ttgctcaaag gtgagggcgt ggcgatattt cacccgcgca
    25981 tcgaccactg gccacaaata accggactct ttcatttgcc gatagccgta attgaactgg
    26041 ttgagcagcg cctcgcgagc cacttcaaag tagcgaaaat agttaccgtg ccaggccacg
    26101 cccatcatat cgacgtcgtg aaagggaatg gtcagctcga cttctgcggt aaatcgggga
    26161 tcgttcagca ccctttactc cttgtcctga atttccggca gttgccagaa atcgaaaaaa
    26221 ttaaaccagt cgagaggcga ctggagcgcg taatgttcca gacgcgcggc gtaatgatcg
    26281 atagcgtttt gcagcgcctg ttggcgttcg ccgcgcggca gctgcagtgg gtcggcaaag
    26341 ctttcgcagt gaatatgcag cttgccgtgc tggcgcaggg cgaaaatcag attcaccggg
    26401 cagcgcaaaa tagaggcgag aataaacggc ccctgtggga aaggcgcagg ctggcccata
    26461 aacgaactcc agcagacgcg ccagtcgccg ccgcgttgcg ggttgacggc gatgcggtca
    26521 ccgacaatcg ccacccattc gccgttatcc agcttctctt tcagcaggat ggcggtttct
    26581 gggccgatat ctgttaccgg catcaggtta atgcctgcct gaggagccat ctcctgcatt
    26641 atctgtttaa agcgttgggc gttttcgcta aacaccagcg cgttaatggt tttgtagccc
    26701 tgaatttttg ccagcgcccg gcacacttcc acatcgccaa gatgcgaggc cagcagcaat
    26761 ttgccgcgcg gatcgcggac gtcaagcgct gcttctgcgc ctggcgcaaa cagcacatca
    26821 cgccctggtt gtagctcgcc gcgccagctg gcgattttgt cgagcatggc attaccgaaa
    26881 cgtagaaagt gctgatagct gttgagtttt gccgcctgtg gttgatgctg tcgtacacgg
    26941 gcgagccagt cttgcgacgc tttgcgcgca cgagaagcag tgagccagta gacgcccacc
    27001 accgggtaaa gcagcgcgct aaacgccgtt cttcccagca gacgccagac cagcagcatc
    27061 aggcgcattc cccataatcc tttcacttca ctctgccgcg cccagtggca ggaagagcgg
    27121 cgcatcagca gtgaaggaat acgcggcaac atgccgaaaa acagacgcgt gtgcatgagc
    27181 gagatgcgga cgttatcttt cagggcatca aaatgcgaaa gcccgtccag tggataggtg
    27241 acgcgggtcg gcacgaaata gctggtattt ccctgccagt agaggcgcac catcacttcg
    27301 gtgtcgaaat ccatccgctt gccgatggtg gcgtgttttg ccagttgcag cgttggcgca
    27361 accggataaa cgcgaaaacc gcacatgctg tctttcagtt gcagggagag cgtttcgatc
    27421 catacccaga cgtgggtgac ccagcgcccg taaagccgtg agcgggggat ggagtcatcg
    27481 taaattggct ggccggagat cagtgccgca ggttgttgtt cagcgagagc cagcagttta
    27541 gggatatctt caatcgcgtg ctgaccgtca gcatccacct gcaccgcatg gctgaaccct
    27601 gcgtctgccg ctgcctgtaa gccacgcatc accgccgcgc ctttgcctgc gttttctgcc
    27661 aggcgaatta aggtcacgcc aggctgttcg gcaagcagac tgtccagttg ctgttgtgtg
    27721 gtggcatcgc tgccgtcatc caccacaata cagggcagat taaatggctt aagacgcgcc
    27781 agcacgcccg gcatcatcgc gccgtggttg tagcagggta tcaacacgca gggagaaaag
    27841 tttaccgaca taggcggatc ttcccgctac tggcggtgtg gcgggcgtca ccgtcgtgac
    27901 gctgatagct aaagctcaga atctggcgtt cttcctgcca gttaagcgcg agcgtgacgc
    27961 ggttttccgg cagcaacgga gactggaatt ttatgttctg aatggagtga aaacgccagc
    28021 caggcgcgag cagattgagc gcgtagctca tcgcccagtc gatttgcgca actcctggga
    28081 gtaatggttg cacggcaaaa tgaccgcgaa accagaacag atccgccctg agatgcagaa
    28141 caatttcaag gtgattcgtt tgtgcctgat ggcgctcgat ttcatggggc ttcatgaaat
    28201 aactcctgta attgcgcata gacacgcttg ttcatactgt ttactggcat ttcatcaata
    28261 acgcgccagt aacgcggaat agcaactggc tccagcgtcg ggcgcagtaa acgccgccag
    28321 gtcatttcct ggctatgccc gccgccgttc tgccattgca gatgagcttc gtcatccagc
    28381 accagcaaaa cgcctatgct ctgacgcccg ccgcgcgtaa cgggaactgc cgccgcctcg
    28441 cgaatgccgt ccagcgccag tagacgttgt tctacttcct ggagtgaaat acgtttttct
    28501 tcaatcttaa cgatgcgccc acgacgcccc atcagatgga actgaccatc ttcgctaaac
    28561 tgtaagatat cgtcgagtaa catgccgtta tcttccgcta tcagcggtga aaagaggcga
    28621 aaggcatcgc cttcggcctg gaactgcata cctgggaggg gtagccagcg gcgttgctct
    28681 tgttcgcggt agcgccaggc gatgacgcca gtttccgtgc tgccgtagat ttcatcaggc
    28741 cagacgcgca gccagctcgc ggtatgttgt acatcctgcc acggcagctc accgccagca
    28801 gagagaagca cctgaacggg tggcggagaa agctgggtat ccaggcgctt caaaaatgcc
    28861 gggctgctga tgaagatata gcgatgctga tgactcaacg cggcaaactg ttcgacatac
    28921 cagagcatgg cggcgtgcag cggtaatccc aacgccatgg gcaggaaaac gcgaaaagtc
    28981 aggccgtaca aatgctgcgg caatacggaa ccgacgacac gacaacccgc cagacgtgcg
    29041 cctaatcgtt cagccagcag ttctgcttca cgatccagca aatgaacagg tttaatcgcc
    29101 cgtttcggct gtcccgtgga accggaggta aacagctcga taaaagcctc tggcgctata
    29161 gcggcaaagg tgaaggattg tgtcgcgatt tgtggagaac ttgctaccaa cagcaatgaa
    29221 ccttgccagt taagttcgct gtcgctaagc acaccatcaa aaagttcacg ttgctcgtta
    29281 agctggataa cacggttatg ccccggaagc accggcgttt ttccggcgtg tagcgtggcg
    29341 agcagggcaa cgataaacag atagctgttc tcaaagcaca gcgcccagcg ttcacccggc
    29401 tgttgctgca agcggcagat aagttgcgcg acatcgtgac gcaaatcgcc cagcgtccag
    29461 gtactttcgt tcagccacgc aatgggtgtg tcatcagggc gtggcgcagt cagccagcgt
    29521 gcaagcggta aggtctgctt catgcgcgat ctcttttcat cacctgacgg cgcagcagcc
    29581 attcaccagc cattaacgtc cccatcaaca aataagcaat catgccgttc caggcggtcc
    29641 ataacgacat atcggcataa agcgcggtaa agagggcaat tccaccgtta atgatgaaaa
    29701 agccgcacca gatttgcgtg acgtggcggg tatagcgcac gcccttttcc gggaggtctg
    29761 gctcctgcaa ccgcgccagc cgctcaacta tcggcattgc cgaccacagc gaaccgccaa
    29821 aaaccgccag catgaccgcg ttaaccacta ccgggtaaaa cagcagtagc tggtgcgttt
    29881 tcaacataaa gctggcgaca cataatgcga tgcccacgac agcgacgagt tgcgtgacgg
    29941 cttgtaaagg ccccgcctgg cgacgggtct ggcgcagacg caacaaaagc agcaacgcca
    30001 ttagcggtaa cagccagtgc aggccgttat gcgctaagcc aaaccaaatc acaaacggcc
    30061 aggcgagcag caataatcca gtcagcagct taatcatcgg tagcgagcga atgcccgaca
    30121 tcagatcagg cttcttgtag caggcgttct acagcctcga cgacatcctg gactgtacgc
    30181 actgctttaa actcttccgg cttgattttc ttaccggttt tcttttgcag gtgcacaatc
    30241 atgtcaacgg cgtcgatgct gtccagttcc agatcttcgt acaggcgcgt ttcaggtttg
    30301 atgtcctgcg gatcgatttc aaacagctta accagcaggg ctgagacttc ctgatagacg
    30361 gtttgttgtt ctgtcatgat ggctttcctc aggcacgttg cgcagcaatg aaagaggcca
    30421 gagtggcgac ggagaaaaag tgctgacgca tctcttcgct ttccgcagag agcacgatgc
    30481 cgtactcatt ttttactgcc agtcccagtt ccagggcatc gatagagtcc agccctaaac
    30541 cgtcgccaaa cagcggcgcg tcggtatcga tatcatcagg cgtcaactcg tccagattga
    30601 gggtgctgat gatgaggttt ttaatttcca gataaagcgc ttgcattatt tattcctgag
    30661 agaggtaaac aaccttgttg taattgaagc aaaagatgcc ggtttaactg ccttgctgcc
    30721 agcgccggtt cttgtgaagt tgcatcgtaa aatttattga tttcaacccg accatggact
    30781 gcaaccgtaa aaaacggccg gtcaggtgga acgtcatacc atttactttg tttatccagc
    30841 agatgctcgc tacagccgat tgtcacgatg cgtaaatcgc tggcgcagcg cacggcaata
    30901 ttcgccgccc cgcgctgcag cgtcattttt tcaccgggct tcgttcgtgt gccttcgggg
    30961 aaaatcagga tagtgtcacc ctgtgccagt cgctgttggc agcggggtaa cagagtttca
    31021 gcttcgctgt tgattaaata atctgtcgcc cgcacgacgc cgccaagaaa aggatttttt
    31081 aacagcgcgc ttttcaccag gcagtcagtt tctggcatga ctgacgccag cagcacgtaa
    31141 tcgatcagcg tgggatgatt ggctacgacg aggcaaccgc gttcctgatg taagatttca
    31201 gcgccatcaa tacgataatc cagtacgcca agccctttcg cgacagttaa aaacaggcga
    31261 aaactggcgg caatgctgcg acgcgccaaa cgacggcggc gggaagtatc ccacaccaaa
    31321 actaacaata tgttaaacca cacgacagaa agcagcaggc cgccgagacc aaacagggca
    31381 aaacataacc cggtcatgac cagtcgccag gtccactcca gacgtgacat cagcttactc
    31441 atctgtggct ccagcgccat tgcacgcgct cgccaggcag tgaaaatgtg tctacatttt
    31501 gtaaatagtg ctgtaaaaac aacatgcttt gcggcagtgt tgtttcattg cccgcaatgg
    31561 ctgactgcgt ttcacattgc cagtcatcgc cagcttcaat caccagcgcg acggcgtacg
    31621 gccaggtcgg catttctgat ggcagttgtg ggtgataaaa ctcgggcagg aaaccgtcaa
    31681 aatcgaccat cagcacgcgc tggtatcccg cttgtagcag gctaagcact tcacacaaac
    31741 cttgctggaa actatcgcga ccagccgaaa gcgatgacga aacgctcggc tgtttggccg
    31801 caatggtcag gttacccacg gaagagttgt gtacggagag cgcgaagtcc gtcggagaaa
    31861 gcgcctgttc ggttgccagt gcatgaacaa tgcggtaatt gcgctccagt tcgccatgac
    31921 gactggtata caaaacggcg tctggttgat ggcggcgtag cattgccaga ccgcattcaa
    31981 cagccagttt gctcccggag ctgaggcggc gggcggtcat cattggcagt tcacttaatt
    32041 ttgccaacgg tgctgtcggg tcaattgcct gaggctgacg tgaccactcc tgccattgca
    32101 gcgtttcact aagtccaggc gctcttgcct gccagtcgag aatgtttaat gcaaatttca
    32161 tcaacgcgcg cccgcggtaa accaaaatta ttaagtgacg attatttctt ttggcatatc
    32221 aaaagtgtat gcccaacgcc taaattatcg tggcgctctg ctacctggaa tcctgcttta
    32281 ttcagatagt cgtaaaattt ctctgcacta tagaaacggc tgttaccgtt tgccatgcag
    32341 gtaaaataaa gtgaggacgc gttcaggcta aacgatgctg cttcaaaccg ctgcgcatcc
    32401 cagaacaact ccatgatgca aacttttgcg ccaggcttca ttacgctggc aattttgctg
    32461 agtatgctaa ttatttgttc gggtgaaaaa cagtctaaaa attggctcat ccaccagata
    32521 tcagcctcgc ccggtaatgg ggcatcgctt agcatatcca ccgcatggaa atctatgcga
    32581 tcagaaaaac ctgcattcgc gatattttct ttcgctagta cgatttgttg tggcagatct
    32641 aataacgtaa cggcgatgtt ttcattgtat ttgcagcaac gcaaagccca cttacccgta
    32701 ttgccgccta catcgtacaa tgttgtgggg ttgttggcga atacataagg cagtgcggcg
    32761 ttaaatgcgc catctgaata gtaatgatca aacgcaaacc agctttcacg agcggcgtct
    32821 ggtaattgcg aaagtgccgg atatatcgtc gaccagtcgc caaatacttt taacccggaa
    32881 ggttttcctt cattgagtga atcggcgagg aagaagagcc cctggtaaca gacatcctgg
    32941 gtgaaatcca tattcacgcg agtcatagtg tcatgcagga ggtaatgacc gattttcgcc
    33001 aggtaataaa ttccctcttt acaggtaata attcgcccgc ttaagcccat atctaacaga
    33061 acgctggtag catattcgtt gatatggcta tgttcggtga tatcagccag cgttgcgcca
    33121 tgtttgcctt gccgatccag atatgaaagt acacccgcat tacgtaggca gagtgctgtt
    33181 tgaaaaagca taggggcaaa ggcaatgcgt tgagcttcgg taatcgcatc cagtgcgcta
    33241 agagaatcct gttcgtacat gttgggcaca acctgttaag agggaattaa attattatcg
    33301 gaccgcaacg gcccgacttt aggaagaatt ataaatctgc gcccgctgac aaattcagtt
    33361 gaatatcttt gtcgagattg cggacccagc gattgtagtt tttatgaatt tgtccgcctg
    33421 acgcttgtag gttctggcta ttttcataat tgatatcgta gctggatgcc gtgtagttaa
    33481 tgcgtatttc cgctgaatga tcgcgtgact gttgacgtgc tttaattatg ccttgtccgg
    33541 cttcggacat aatccatttg cgttcaacgc cagctttcag aatcgctttt ttcacttgtt
    33601 cctgggtatg accagcgctg acggttgagt gaacctgatc aattggcgca gtacgcgcgc
    33661 aacctgctaa agcacccaca acagcaatag ccgcaaacca tttcatgtaa tgtctcatta
    33721 gaaccccatt aaagtattgt ttttacgagt aaagcaagtt ttaactgtaa ttgctggcat
    33781 cactattctt tatttagaga aaaaattact tgtgagataa gtcagtaagt gcacatcaga
    33841 gtaaaaatct ctgaaatgac taaattactg ccagcaggga ccaactataa gtgagtgaga
    33901 aaaatgaggc aataaaaata acacaatgtt atcagtgcat tctttgagaa ggatttaatc
    33961 gctattcggc gtttatatat atttgactta aggaatggtt tttttaaatt aattttctga
    34021 ataatgtttc atcatttcct caaatcaaat ggccactgtt agataaacgg tggccattgc
    34081 ctgctcagga ggtgctatta acccggtaac cgacttttcg gtctgcccgg ccccagtaaa
    34141 atcgccagtt tgctgccgcc tttcgtcgtt tccatccaga ttttacacac gctggttaat
    34201 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccaaattaa caatgaaaga
    34261 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggttc taaaatattg
    34321 ccgatgacca tatggaccac taaaaacaat gcgccgacca gaatacattc gtaaacacca
    34381 ttaaacagca gcacctgaat cattggcggt acggcggaaa ttaccgcgcc gatattgggc
    34441 acgtagttaa gcaaaaacgc cagtactgcc cacatcagcg caaactgtac ccccatcagc
    34501 tccagcccca gccagacgat gacacccgtc cataaactga gtagcgtctt taacgccaga
    34561 taatgcgaaa cgcctttaag tgcgcggtgt aatcccgcga tgtgaatctg tgggttattc
    34621 agcgcaaaac gcattttgta agggacgtgg cgcacttcaa acagcataaa aactacggtc
    34681 atcaccagca aaagcacgct cgccattgcc ccggaaagcc cggtcattag cgctgtggtg
    34741 aaggtaacca ctttttccga gtccatccgc tgcagcattc gctccggcga catatgcaaa
    34801 ttaagaaaag gcaacatctc ctgcaattta aaaagtttgc gcgtcagctc cttattaaac
    34861 ttcggcagca tagagataaa ttcgttaaac gatgccgcca gtacgccgac cagcgcggtt
    34921 agtgcgatca gcatcaccac cactacaatc gtaatggcaa cggggcgttg tactccccga
    34981 cgaataaacc aggtgacgag cgggttgagg acgatggcaa aaaacagcgc cagtaacagc
    35041 tgcacaatga tatctgccgc tgcgtgaatg cccgcgagga tcactaccag cgaggccagc
    35101 ttgagcagaa tgtgcatgcc cgttttatcg ggttgagggg tttccattgg ggcttccttg
    35161 tgactttttg tattaagtgt agcgggagtc acgacggcaa tattctgatt cttactgctg
    35221 attttcacgt cagcacgtgg taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat
    35281 gcccgaaccc gtagccgaac ccgcgctaaa cggattgcgc ctgaatttgc gcattctctc
    35341 cattgtcatg tttaacttcg ccagctacct caccatcggg ttgccgctcg ctgtattacc
    35401 gggctatgtc catgatgtga tgggctttag cgctttctgg gcaggattgg ttatcagcct
    35461 gcaatatttc gccaccttgc tgagccgtcc tcatgccgga cgttacgccg atttgctggg
    35521 ccccaaaaag attgtcgtct tcggtttatg cggctgcttt ttgagcggtc tgggatatct
    35581 gacggcagga ttaaccgcca gtctgcccgt catcagcctg ttattacttt gcctgggacg
    35641 cgtcatcctt gggattgggc aaagttttgc cggaacggga tcgaccctgt ggggtgttgg
    35701 cgtggttggc tcgctgcata tcgggcgggt gatttcgtgg aacgacattg tcacttacgg
    35761 ggcgatggcg atgggtgcgc cgttaggcgt cgtgttttat cactggggcg gcttgcaggc
    35821 gttagcgtta attattatgg gcgtggcgct ggtggccatt ttgttggcga tcccgcgtcc
    35881 gacggtaaaa gccagtaaag gcaaaccgct gccgtttcgc gcggtgcttg ggcgcgtctg
    35941 gctgtacggt atggcgctgg cactggcttc cgccggattt ggcgtcatcg ccacctttat
    36001 cacgctgttt tatgacgtta aaggttggga cggtgcggct ttcgcgctga cgctgtttag
    36061 ctgtgcgttt gtcggtacgc gtttgttatt ccctaacggc attaaccgta tcggcggctt
    36121 aaacgtagcg atgatttgct ttagcgttga gataatcggc ctgctactgg ttggcgtggc
    36181 gactatgccg tggatggcga aaatcggcgt cttactggcg ggggccgggt tttcgctggt
    36241 gttcccggca ttgggtgtag tggcggtaaa agcggttccg cagcaaaatc agggggcggc
    36301 gctggcaacc tacaccgtat ttatggattt atcgcttggc gtgaccggac cactggctgg
    36361 gctggtgatg agttgggcgg gcgtaccggt gatttatctg gcggcggcgg gactggtcgc
    36421 aatcgcgtta ttactgacgt ggcgattaaa aaaacggcct ccggaacacg tccctgaggc
    36481 cgcctcatca tcttaaaact tactgaataa ccagcgtatt aatgatgttt tctgcggtgg
    36541 tctgcgcttt ttgctgatcg tcagctggca gagtaatttg catggtcagc agttgattac
    36601 ccacgttacc cagaataacg gaagagtacg ccgtctggcc tttcgcggag ataatactgt
    36661 ctaactgctg cattttgtga cctttcagct caatggcttt attggtgacc acttgcagct
    36721 gtggatcgcg gctacgttgc tgatcttcca gacgcttcgc cagcaccgcc agatcttctt
    36781 tcggatcatc gcccatgatg acgatgactg ctttctgccc ggtggcgtcg gaccagacat
    36841 gcatgttatt ggcctgcgtt cccagcttac cgctctggtc ggtcatatcc gctggcagcg
    36901 agaaacttaa cttgccatca agcaggttga cgggattccc ggtagcgtta ctttccgcga
    36961 ccgaaccctg cgccgtagcg ttagtgtctt tatcatcaca ggccgcaagc cccataacca
    37021 gcaggccaat tccgacatat ttaaccagat tgcgcattga cttcttcctt tcgataaacg
    37081 gccataacgg ctcattcatc catcttatca caactctgat aacgaacctt taactcgcct
    37141 gcaaagcgtt gatttcggat ttatctgcca gtcttttcaa cagcatattg agtaatacgc
    37201 catacattgg caggaagaaa acgatactga ttaacacttt gaaacagtaa tcgaccagcg
    37261 cgatttccat ccagtgttca gccataaagg catccgggct acgccagaag gcaatgaaga
    37321 aaaaggccag cgtgtcgctg acgttaccga acagtgtgga cgctgtcggt gccagccacc
    37381 agcggcgact ctgacgcagg cggttaaaaa cgtgcacgtc gaggatctgt cccagcgcgt
    37441 aggccatgaa actggcggtg gcgatacggg cgacaaacag gttgaagtgg gcgagtgcgc
    37501 cgaatccctg ccatgaaccc atatagaata gcgacgagat gacgtaggag atcagcaatg
    37561 ccgggatcat caccgcaaag atgatacgac gggccagcgg tgcgccaaaa atacgtacgg
    37621 tcaggtcggt agcgagaaag ataaacggaa agctaaacgc gccccaggtg gtatggaaac
    37681 caaaaatgga gacgggaagc tgaaccagat agttactgga ggtgatcacc agcagatgaa
    37741 ataacgataa ccagaacaac gccttatagc gttgagtttg cgagaaaacg ttcatattgt
    37801 acctttttga ttaaccattg gggtgaggga acccaatacg tacgacacgt ctgttacctt
    37861 aacataataa cgtgcttacc cttttttcga gccgccgcat gatactgctt tacgttgaca
    37921 atgcaatggt taattttcac gcaatcgtta acctggtttg cttacggact cacagggcgt
    37981 aaactagcgc cgttttttta tgtgatgaga agaaaatgac cgatctcttt tccagccctg
    38041 accacacact cgacgcactt ggcctgcgct gcccggaacc agtcatgatg gtgcgcaaaa
    38101 cagtgcgcaa tatgcagcca ggtgaaacat tgctgattat tgctgacgat ccggcgacga
    38161 cccgcgatat ccctgggttt tgtaccttta tggaacacga actggttgcc aaagagacgg
    38221 atggactgcc ttatcgttat ttgattcgta aaggcggttg acgggggcag attggcttcg
    38281 atgccgcctt ttcccctcac cctaaccctc tccccagagg agcgagggga ccgatcgcgc
    38341 tcaatgttgc gatcggtttg ccttatctcc tgcgcaacaa tcttaacgca ttcgctgtca
    38401 ccagcaccgt cgcacccgta tctgccagca ctgccagcca tagtccggtc atccctaaca
    38461 gcgtggtgac gaggaagatc cctttcagcc ccagcgcaat agtgatgttc tggcggatat
    38521 tggcgtgagt ggcgcgtgcc agttcaatca tttgcaccag accgcgcagg tggttatggg
    38581 ttaatgctgc gtcggcggtt tccagcgcca cgtctgtgcc gctgcccatt gcaatcccga
    38641 tggcggcagc tttcatcgct ggcgcgtcgt taataccgtc accgaccatc gccagcggcg
    38701 catgttgatt cagctcggtc accgctttga ctttatcttc cggcaacagg cccgctttaa
    38761 actccagccc cagctccccg gcaattgccg ccgctgcgcg tggattatcg ccggtgagga
    38821 tcaccccttt gacgcccagc gcgttcagtt cactgatggc agttgcagca tcggcgcgca
    38881 gggtatcctg caatgcaatg atacccagca cgtcatcatt acgtactacc agcacaaccg
    38941 tttgcccggc gctttccagt tcattaatca aaccagcaaa tgcatcagcg ggatgtttcc
    39001 cggcagcgca tatcaacacg cgctcaccgt taacctgcgc ttcaatgcca gacccgacca
    39061 gcgcccgctg tgattcggcg gcgggaatgg cgagtgcagc aacctgtgct tcgcgcacga
    39121 tggcctgcgc cagtggatgc gtcgcgcctt gctcgaccgc cgccgccagt gtcagcagtt
    39181 cagattcact aatacccgtt gccggatgaa tcgcggtaac gcgcggttta ccgacggtca
    39241 gcgtaccggt tttatcaaac gccacctggg taacacgacc cagctgttcc agcgccgctc
    39301 cgcctttaat caacgcccca cgacgcgctg ccgccgccag cccggaggta atcgccgcag
    39361 gcgttgagat aactaacgca cacgggcagc caatcagcag cagcgtcagc cctttataaa
    39421 tccactcctg ccagctggtg gcaaacagca gcggtggcac cagcgtcacc agcagagcga
    39481 cggccataat cgcgggcgtg taaatacggc tgaaacggtc gataaaccgc tcaatgggtg
    39541 cgcgacgctc ttcggcttct tcgatcagtt tcagaatccg gtcaatggcg ctggcacccg
    39601 gttctgacag cacttccagc gttaccagac ggtctacgct ggtggctcct gcaggaactt
    39661 tatcgcccgt tgcgcgctcc accggaatag attcgccggt cagggcgctt tcatcaaaac
    39721 tggcaaacgg tgagagcagt ttaccgtcgg caggcaaacg tccacctgcg gcgacttcaa
    39781 tcacatcgcc agggcgcagg ctgttaatcg ccacctcttc ccgctcaccg ttacgcaggc
    39841 gcgtggcggt ttctggtttc agcgccatta acgcgctgac cccttgacgc gcgcggctgg
    39901 cggcccagcc ttccagtcgt tcaccaatca aaaacagcag caacaccatc gcagcttcag
    39961 ccgttgcgcc aataaacagt gcaccaatag cggctacgct cattaaggtt tcaatggcga
    40021 agtagctgcc ggatttgatc agccgtaatg cctgacgagc aatcgggtac agcccaacca
    40081 gcgtggtcgc gataaacgcc agttgcccga acggatgatt aaattgctcc aaaccccagc
    40141 tgattgccat catcacgata agcgtaatca gcggcagatt ctctttcagg cgcgatgctt
    40201 gtggctcgtc ggcggcctgc tcatcgcgca gggaatagcc cgctttttgc accgcagatt
    40261 caacttgtgc acggatgtca ttgtcggcat caaccaccag tttttcggtg gcgaacaaca
    40321 cctgtacctg attcacgcct gcaagctggc gcacggcatt ttctaccttg cgcgcacagg
    40381 cggcgcagtc catgccgctg actttccagc tatagcgggt gccggagacg ttttcagaga
    40441 gagttggcgt gctggaacat gcgccgtcgc agcaacagtc gttggcgttc tgtaccgtgg
    40501 ttagcggttt gaacgcagca aattgagggg ctttcttgcc gtgattgtca ggagtcgaca
    40561 tggcatcctc cggttaagtt ttttctcatt aaccgaagga tacactctgg agtcgactcc
    40621 agagtcaagt tttatcagag atacagcgag cggacgatga ggaagtgacc ggcaaagtag
    40681 caggccgcag caatcgcgtt gtccgcacgg aagcgtcggc gatagtgact acccagccag
    40741 acaaagttac tgataaacag caacgaagcg ccgacaaacg cagagagtgc cggagcggtc
    40801 ggacggaaga accacagttc acctgccagc cacaccatca ccagcgtcat gccgataaac
    40861 gtgcagatag gccaacgata ctcttccagc cgcgtccaga taatcgccag taacagcgca
    40921 cccagaacca gcaacaccag tggcagaggc cagaagaaag agagcgtcat ctgactggcg
    40981 aaatagatgg tgtacagcag gtgcgagagg aaaaacgcgc cgatggcgta catcagacgt
    41041 tgacgtggca acagggttag cgcatcgccc agcagtgagg cgcacagccc tgccagcacc
    41101 agatagctaa tggcgtcgaa catcggcgct tgccaggcca gtaacagcag gagaagaagg
    41161 gttaacggtt taaacaccca gcgttgccag gttggcccac gatacgatgc atccacagat
    41221 agccatgcgg aaagacagac agcgataaac gaccaaagca tcttagttcc ttgaattcgt
    41281 tattttttcg taagcggaat gcctacggtc ttgcttccag tttagtggcg agggtggacg
    41341 gatgacaaca ccataactgg cagggtatgc ttatttccgc attttccgat gagggataaa
    41401 gatgagcaaa ccacctcttt tctttattgt tatcattggc ttaatcatcg tcgccgcatc
    41461 gtttcgtttt atgcaacagc gacgggaaaa agctgataat gatatggcac cactccagca
    41521 aaagctggtg ttggtgagca acaagcggga aaaaccgatt aacgatcgcc gttcgcgcca
    41581 gcaggaagtg actccggcag gcaccagtat gcgctatgag gcaagcttca aaccgcaaag
    41641 cggaggaatg gagcagacgt ttcgcctcga cgcccagcag taccacgccc tgacagtggg
    41701 cgataaaggt acgctgagct ataaaggaac gcgctttgtt agctttgttg gcgaacaata
    41761 aggttatttt ttaactttaa atttcttctg ccagaccagc agttcaaaca cgccgaaaag
    41821 gaaaatccgc accttttcgg cggtggtcat ctgcgggcca tctttcggta aagtggattt
    41881 caatagcgcc agttgcatac catgcatcag cacggtaaaa atcagcgcca cgttaacgaa
    41941 aatattcagc gggcgcggga agggatgcac caggttgagg attaaaaatc cccaaacaca
    42001 gagcattaac aaacgaccaa tattaatcag catcactttc tccttgtgct tcgcgttgat
    42061 acagccgata agccacctga cccgccactt tttcccgatg taacgaccag tttgccggaa
    42121 cggtgggcag accgttttcg acttcgcttt cgacataaat cagggtttcg tcagccagcc
    42181 agccgttatc ttccagtaaa tttatcgtct cttctaacaa accacggcgg aacggtggat
    42241 cgacaaacac gatattatgc ggtgtgcctt tttgcgccag gaatgacatt gcgttgctgt
    42301 tcaccacgcg tgcattgcct gcttttagtg tcgccagatt cttaattaac tgctgagaaa
    42361 ctgcacgatc catctcaatt aacgtggccc ccgcagcgta gcgcgataac gcttccagcc
    42421 ccagcgcgcc gctcccggcg aagcaatcca gacattgggc gtcaacaatg accggagcca
    42481 gccagttaaa caacgtttcg cgtacgcggt cggtggtggg gcgcagacct gggctatcag
    42541 gaaccgggag tttacggcct cgccactgcc cgccaataat gcggatttgg ccgctgccgg
    42601 aatgattcgg ttttttcatg ataaattgct caatccgcca gataacacat acttgcaggc
    42661 ggtgatgtga attaagttaa gtgatagact atttcatcat ttttttagct gctatgtaca
    42721 tagcgttaac gctgtgccat gaagcaacag cgaggagtgt agtcgcaaat ggcgaaagaa
    42781 aaaaaacgtg gctttttttc ctggctgggc tttggtcaaa aagagcagac cccggaaaaa
    42841 gagacagaag ttcagaatga acaaacggtt gtagaagaaa tcgttcaggc gcaagagcct
    42901 gtgaaggctt ctgaacaagc cgtcgaagag cagccgcagg cgcatactga ggccgaggcg
    42961 gaaacttttg ctgccgacgt tgtggaagtc actgaacagg ttgctgaaaa tgaaaaagcg
    43021 cagcctgaag cggaagtcgt tgcacaaccg gaaccggtcg tagaagaaac gccggagcca
    43081 gtggctatcg aacgtgaaga gctgccgttg ccggaagacg tcaacgccga agaggtttcg
    43141 ccagaagagt ggcaggctga agcggaaacc gtagagattg tcgaagcggc ggaagaagaa
    43201 gcggctaaag aagaaatcac cgacgaagag ctggaagcac aggcgctggc tgccgaagca
    43261 gcagaagagg cggtgatggt ggttcctccg gcagaagaag agcagccggt ggaagaaatc
    43321 gctcaggagc aggaaaaacc gaccaaagaa ggttttttcg cgcgcctgaa acgcagcctg
    43381 ttaaaaacca aagaaaatct cggttccgga tttatcagcc tgttccgcgg taaaaaaatc
    43441 gacgatgatc tgtttgagga gctggaagag cagcttttga tcgccgatgt gggcgtggaa
    43501 accacacgta aaattatcac caatctgacg gaaggcgcat cccgcaagca gcttcgtgat
    43561 gccgaggcgc tctatggcct gctgaaagaa gagatgggtg agattctggc gaaagtcgat
    43621 gagccgctga atgttgaagg taaaacgccg ttcgtgatcc tgatggtggg cgtcaacggt
    43681 gtgggtaaaa ccacgacgat tggtaagctg gcacgtcagt ttgagcagca gggtaaatcg
    43741 gtgatgctgg cggcgggcga tactttccgt gcagcagcgg ttgaacagct tcaggtctgg
    43801 gggcagcgca acaatattcc ggtgattgcc cagcataccg gggcggattc cgcctctgtt
    43861 atcttcgacg ccattcaggc ggctaaagcg cgtaatatcg acgtcctgat tgccgatacc
    43921 gccggacgcc tgcagaacaa atcgcacctg atggaagagt tgaagaaaat cgtccgcgtg
    43981 atgaagaaac tcgacgttga agcgccgcat gaagttatgc tgactatcga tgccagcacc
    44041 gggcagaacg cggtaagcca ggccaaactg ttccatgaag ccgttggctt aaccggcatc
    44101 acgctaacga aactggacgg cacggcgaaa ggcggggtaa ttttctcggt ggctgaccag
    44161 tttggtatcc ctatccgcta cattggtgtc ggcgaacgta ttgaggattt gcgtccgttt
    44221 aaggcggacg actttataga ggcacttttt gcccgagagg attaacaatg attcgctttg
    44281 aacatgtcag caaggcttat ctcggtggga gacaggcgct gcagggcgtt acgttccata
    44341 tgcagccggg tgagatggcg tttctgaccg gtcattccgg cgcagggaaa agtaccctcc
    44401 tgaagctgat ctgtgggatt gagcggccca gcgccgggaa aatctggttt agcggccatg
    44461 acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg ccgccagatt ggcatgattt
    44521 tccaggatca ccatctactg atggaccgta ctgtctacga taacgtggcg atcccgctga
    44581 ttatcgccgg tgccagcggt gacgatattc gccgccgggt gtcggcggca ctggataaag
    44641 tcgggctact ggacaaagcg aagaacttcc ctattcagct ttctggcggt gaacaacagc
    44701 gtgttggcat tgcccgcgcg gtggtgaaca agcccgcggt actgctggcg gacgaaccga
    44761 ctggtaactt ggacgacgcg ttgtcggaag ggattctacg tctgtttgaa gagtttaacc
    44821 gcgtaggggt aaccgtattg atggcaacgc acgacatcaa cctgatttca cggcgttcct
    44881 atcgcatgct caccctgagc gatggtcact tgcatggagg cgtgggccat gaataagcgc
    44941 gatgcaatca atcatattcg gcagtttggc gggcgtcttg atcgcttccg taaatcggtc
    45001 ggcggctcag gcaacggcgg tcgtaacgca ccgaaacgcg cgaaatcctc gccaaaaccg
    45061 gtaaatcgca aaaccaacgt tttcaacgaa caggtgcgct atgccttcca cggcgcattg
    45121 caggatctga aaagcaaacc gttcgccaca tttttaacgg tgatggttat cgccatctcc
    45181 ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg ttaaccaggc ggcgacgcag
    45241 tattatccgt caccgcaaat cactgtttat ctgcaaaaaa cgctggacga tgacgccgct
    45301 gcgggcgtgg tggcacagtt gcaggccgag caaggcgtag agaaagtgaa ctatctttct
    45361 cgtgaagacg cactgggcga gttccgtaac tggtctggtt ttggtggcgc gctggatatg
    45421 ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc cgaagctcga ctttcagggg
    45481 acggagtctc tgaatacgct gcgtgatcgt atcacgcaga ttaacggcat tgacgaagtg
    45541 cggatggatg acagctggtt tgcccgtctg gcggcgttga ccgggctggt cgggcgcgtt
    45601 tcggcgatga tcggcgtgtt gatggtggcg gcagtgttcc tcgtcatcgg taacagtgta
    45661 cgcctgagta tctttgctcg tcgtgactcc attaacgtgc agaaactgat tggtgcaacg
    45721 gatggtttca tcctgcgccc gttcctgtac ggtggcgcat tgctggggtt ttctggcgca
    45781 ttgttgtcat taattttatc agaaattctg gtactgcgat tgtcatcggc ggttgcggaa
    45841 gtggcacagg ttttcggaac gaagtttgat atcaatggtt tatcgttcga tgaatgcctg
    45901 ctgttgctgc tggtatgctc gatgattggc tgggtggcag cgtggcttgc cacggtacaa
    45961 catttacgcc actttacgcc tgaataataa aagcgtgtta tactctttcc ctgcaatggg
    46021 ttccgtagca gggaaagaga ccccgttgtc tcttcccggt atttcatctc tatgtcacat
    46081 tttgtgcgta atttattcac aagcctgcat tgaacttgtg gataaaatca cggtctgata
    46141 aaacagtgaa tgataacctc gttgctctta agctctggca cagttgttgc taccactgaa
    46201 gcgccagaag atatcgattg agaggatttg aatgactgac aaaatgcaaa gtttagcttt
    46261 agccccagtt ggcaacctgg attcctacat ccgggcagct aacgcgtggc cgatgttgtc
    46321 ggctgacgag gagcgggcgc tggctgaaaa gctgcattac catggcgatc tggaagcagc
    46381 taaaacgctg atcctgtctc acctgcggtt tgttgttcat attgctcgta attatgcggg
    46441 ctatggcctg ccacaggcgg atttgattca ggaaggtaac atcggcctga tgaaagcagt
    46501 gcgccgtttt aacccggaag tgggtgtgcg cctggtctcc ttcgccgtcc actggatcaa
    46561 agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc gtcaaagttg cgaccaccaa
    46621 agcgcagcgc aaactgttct tcaacctgcg taaaaccaag cagcgtctgg gctggtttaa
    46681 ccaggatgaa gtcgaaatgg tggcccgtga actgggcgta accagcaaag acgtacgtga
    46741 gatggaatca cgtatggcgg cacaggacat gacctttgac ctgtcttccg acgacgattc
    46801 cgacagccaa ccgatggcac cggtgctcta tctgcaggat aaatcatcta actttgccga
    46861 cggcattgaa gatgataact gggaagagca ggcagcaaac cgtctgaccg acgcgatgca
    46921 gggtctggac gaacgcagcc aggacatcat ccgtgcgcgc tggctggacg aagacaacaa
    46981 gtccacgttg caggaactgg ctgaccgtta cggtgtttcc gctgagcgtg tgcgccagct
    47041 ggaaaagaac gcgatgaaaa aactgcgcgc tgctatagaa gcgtaatttc cgctattaag
    47101 cagagaaccc tggatgcgag tccggggttt ttgttttttg ggcctctaca ataatcaatt
    47161 tcccctccgg caaaacgcca atccccacgc agattgttaa taaactgtca aaatagctat
    47221 tccaatatca taaaaatcgg gtatgtttta gcagagtatg ctgctaaagc acgggtagtc
    47281 atgcataaaa cgaaataaag tgctgaacaa caacatcaca acacacgtaa taaccagaag
    47341 aatggggatt ctcaggatga acataaaggg taaagcgtta ctggcaggat gtatcgcgct
    47401 ggcattcagc aatatggctc tggcagaaga tattaaagtc gcggtcgtgg gcgcaatgtc
    47461 cggtccggtt gcgcagtacg gtgaccagga gtttaccggc gcagagcagg cggttgcgga
    47521 tatcaacgct aaaggcggca ttaaaggcaa caaactgcaa atcgtaaaat atgacgatgc
    47581 ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg gcattaaata
    47641 cgtgattggt cacctctgtt cttcatcaac gcagcctgcg tctgacatct acgaagacga
    47701 aggcatttta atgatcaccc cagcggcaac cgcgccggag ctgaccgccc gtggctatca
    47761 gctgatcctg cgcaccaccg gcctggactc cgaccagggg ccgacggcgg cgaaatatat
    47821 tcttgagaaa gtgaaaccgc agcgtattgc tatcgttcac gacaaacagc aatacggcga
    47881 aggtctggca cgagcggtgc aggacggcct gaagaaaggc aatgctaacg tggtgttctt
    47941 tgatggtatc accgccgggg aaaaagattt ctcaacgctg gtggcgcgtc tgaaaaaaga
    48001 gaatatcgac ttcgtttact acggcggtta tcacccggaa atggggcaaa tcctgcgtca
    48061 ggcacgcgcg gcagggttga aaactcagtt tatggggccg gaaggcgtgg ctaacgtctc
    48121 gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc cgaagaacta
    48181 cgatcaggtt ccggcgaaca aacccattgt tgatgcgatc aaagcgaaaa aacaggaccc
    48241 aagcggcgca ttcgtttgga ccacctacgc cgcgctgcaa tctttgcagg cgggcctgaa
    48301 tcagtctgac gatccggctg aaatcgccaa atacctgaaa gcgaactccg tggataccgt
    48361 aatgggaccg ctgacctggg atgagaaagg cgatctgaaa ggctttgagt tcggtgtgtt
    48421 tgactggcac gccaacggca cggcaaccga tgcgaagtaa cagatagata aatgaaaatg
    48481 cccgatgcgc tgaactgcca tcgggttttt ttgtgtttgt cgaaatggtt gtagaaagat
    48541 taaacaatcc aggatatgct taatgtacgg tgacataccg tacatattat acggagcagg
    48601 agagttcaca tgtctgctgt taaaaagcag cgtatcgatc tgcgtttaac tgacgatgac
    48661 aaaagtatga ttgaggaagc ggcagcgata tctaatcagt ccgtcagcca atttatgttg
    48721 aacagcgctt cgcagcgggc tgcggaagtg attgaacagc atcggcgggt gatcctcaat
    48781 gaggaatcct ggacgcgggt gatggatgcg ctgagtaatc caccgtcacc aggtgaaaag
    48841 ctaaaacgtg cggcaaaacg tcttcaggga atgtaataat ttatggatga tctgacgata
    48901 gagattctga ccgatgatgc agattatgat ctacagcgat tcgactgcgg cgaggaagcg
    48961 ttaaatctct ttctgacgac acatctcgtt cgtcaacatc gcaacaaaat tctgcgagcg
    49021 tatatccttt gtcgcaacac tccagaacgt caggtgctgg gatattacac attatgcggc
    49081 agttgttttg aacgagccgc attgccctcg aaatcgaaac agaaaaaaat tccctacaaa
    49141 aatattccca gcgttactct tgggcgtctg gcaattgatc gttcattaca ggggcaggga
    49201 tggggagcaa cactggttgc tcatgccatg aaagtcgtct ggtcagcctc tttagcggta
    49261 ggtattcacg gtctttttgt cgaggcgctg aatgaaaaag cccatacgtt ttataaatcg
    49321 ctgggcttta tccctttagt cggggaaaac gaaaatgcgt tatttttccc aaccaaatcc
    49381 attgaactgc tttttacaca gagcgattaa cgcttctccc agccgccctg ctgtgccgta
    49441 aatcccagtg cctgcataaa cgccgtcatc acaccgcgat cttccacgcc tgcatccgcc
    49501 atccaccagc atgaaacgcc aggattgtta cgcaaaacct cttccagcag atattgcccc
    49561 acaccgcgac gtcgagtgac ttcccgcacg cgcagggaat ccagtgcccc ctcggtgccg
    49621 cttaaggtta cccgcacggc agcaagcagg cgctcgttaa aacgcgcggc gtagatgcgg
    49681 tggttatcgt caacctgtaa agaggaaggg gaatactccg gccagatctt ttgcaggtca
    49741 atccggtcct ggtcgctaaa tttttctaat cgaatgatgg tcagcttcat gggtaacccg
    49801 tgtaaatcac aaaagtggaa ccagtgtagc gaaataattt aatcggaggc tttctctttt
    49861 ttatttcttt tggcaggtga ttaatttc
//
LOCUS       DAZFKY010000009        49644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFKY010000009 DAZFKY010000000
VERSION     DAZFKY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            53..685
                     /gene="comR"
                     /locus_tag="ACHKCL_000490"
     CDS             53..685
                     /gene="comR"
                     /locus_tag="ACHKCL_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5761532.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(747..1286)
                     /gene="ycfJ"
                     /locus_tag="ACHKCL_000491"
     CDS             complement(747..1286)
                     /gene="ycfJ"
                     /locus_tag="ACHKCL_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5761533.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="ACHKCL_000492"
     CDS             complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="ACHKCL_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5761534.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3217..3759)
                     /gene="ycfP"
                     /locus_tag="ACHKCL_000493"
     CDS             complement(3217..3759)
                     /gene="ycfP"
                     /locus_tag="ACHKCL_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5761535.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3782..4807)
                     /gene="nagZ"
                     /locus_tag="ACHKCL_000494"
     CDS             complement(3782..4807)
                     /gene="nagZ"
                     /locus_tag="ACHKCL_000494"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5761536.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4818..5642)
                     /gene="thiK"
                     /locus_tag="ACHKCL_000495"
     CDS             complement(4818..5642)
                     /gene="thiK"
                     /locus_tag="ACHKCL_000495"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5761537.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5623..6264)
                     /gene="lpoB"
                     /locus_tag="ACHKCL_000496"
     CDS             complement(5623..6264)
                     /gene="lpoB"
                     /locus_tag="ACHKCL_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5761538.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6278..6655)
                     /gene="ycfL"
                     /locus_tag="ACHKCL_000497"
     CDS             complement(6278..6655)
                     /gene="ycfL"
                     /locus_tag="ACHKCL_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5761539.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="ACHKCL_000498"
     CDS             complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="ACHKCL_000498"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5761540.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7357..9546
                     /gene="fhuE"
                     /locus_tag="ACHKCL_000499"
     CDS             7357..9546
                     /gene="fhuE"
                     /locus_tag="ACHKCL_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5761541.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9606..11039)
                     /gene="ptsG"
                     /locus_tag="ACHKCL_000500"
     CDS             complement(9606..11039)
                     /gene="ptsG"
                     /locus_tag="ACHKCL_000500"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5761542.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11334..12131)
                     /gene="ycfH"
                     /locus_tag="ACHKCL_000501"
     CDS             complement(11334..12131)
                     /gene="ycfH"
                     /locus_tag="ACHKCL_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5761543.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12142..13146)
                     /gene="holB"
                     /locus_tag="ACHKCL_000502"
     CDS             complement(12142..13146)
                     /gene="holB"
                     /locus_tag="ACHKCL_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5761544.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13143..13784)
                     /gene="tmk"
                     /locus_tag="ACHKCL_000503"
     CDS             complement(13143..13784)
                     /gene="tmk"
                     /locus_tag="ACHKCL_000503"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5761545.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13774..14796)
                     /gene="yceG"
                     /locus_tag="ACHKCL_000504"
     CDS             complement(13774..14796)
                     /gene="yceG"
                     /locus_tag="ACHKCL_000504"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5761546.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14799..15608)
                     /gene="pabC"
                     /locus_tag="ACHKCL_000505"
     CDS             complement(14799..15608)
                     /gene="pabC"
                     /locus_tag="ACHKCL_000505"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5761547.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15728..16969)
                     /gene="fabF"
                     /locus_tag="ACHKCL_000506"
     CDS             complement(15728..16969)
                     /gene="fabF"
                     /locus_tag="ACHKCL_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5761548.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17057..17293)
                     /gene="acpP"
                     /locus_tag="ACHKCL_000507"
     CDS             complement(17057..17293)
                     /gene="acpP"
                     /locus_tag="ACHKCL_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5761549.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17504..18238)
                     /gene="fabG"
                     /locus_tag="ACHKCL_000508"
     CDS             complement(17504..18238)
                     /gene="fabG"
                     /locus_tag="ACHKCL_000508"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5761550.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18251..19180)
                     /gene="fabD"
                     /locus_tag="ACHKCL_000509"
     CDS             complement(18251..19180)
                     /gene="fabD"
                     /locus_tag="ACHKCL_000509"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5761551.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19196..20149)
                     /gene="fabH"
                     /locus_tag="ACHKCL_000510"
     CDS             complement(19196..20149)
                     /gene="fabH"
                     /locus_tag="ACHKCL_000510"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5761552.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20217..21287)
                     /gene="plsX"
                     /locus_tag="ACHKCL_000511"
     CDS             complement(20217..21287)
                     /gene="plsX"
                     /locus_tag="ACHKCL_000511"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5761553.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21368..21541)
                     /gene="rpmF"
                     /locus_tag="ACHKCL_000512"
     CDS             complement(21368..21541)
                     /gene="rpmF"
                     /locus_tag="ACHKCL_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5761554.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21593..22114)
                     /gene="yceD"
                     /locus_tag="ACHKCL_000513"
     CDS             complement(21593..22114)
                     /gene="yceD"
                     /locus_tag="ACHKCL_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5761555.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22313..22897
                     /gene="yceF"
                     /locus_tag="ACHKCL_000514"
     CDS             22313..22897
                     /gene="yceF"
                     /locus_tag="ACHKCL_000514"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5761556.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22998..23957)
                     /gene="rluC"
                     /locus_tag="ACHKCL_000515"
     CDS             complement(22998..23957)
                     /gene="rluC"
                     /locus_tag="ACHKCL_000515"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5761557.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24530..27715
                     /gene="rne"
                     /locus_tag="ACHKCL_000516"
     CDS             24530..27715
                     /gene="rne"
                     /locus_tag="ACHKCL_000516"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5761558.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27910..28863)
                     /gene="flgL"
                     /locus_tag="ACHKCL_000517"
     CDS             complement(27910..28863)
                     /gene="flgL"
                     /locus_tag="ACHKCL_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5761559.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLENSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28875..30518)
                     /gene="flgK"
                     /locus_tag="ACHKCL_000518"
     CDS             complement(28875..30518)
                     /gene="flgK"
                     /locus_tag="ACHKCL_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5761560.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30584..31525)
                     /gene="flgJ"
                     /locus_tag="ACHKCL_000519"
     CDS             complement(30584..31525)
                     /gene="flgJ"
                     /locus_tag="ACHKCL_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5761561.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31525..32622)
                     /gene="flgI"
                     /locus_tag="ACHKCL_000520"
     CDS             complement(31525..32622)
                     /gene="flgI"
                     /locus_tag="ACHKCL_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5761562.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32634..33332)
                     /gene="flgH"
                     /locus_tag="ACHKCL_000521"
     CDS             complement(32634..33332)
                     /gene="flgH"
                     /locus_tag="ACHKCL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5761563.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33385..34167)
                     /gene="flgG"
                     /locus_tag="ACHKCL_000522"
     CDS             complement(33385..34167)
                     /gene="flgG"
                     /locus_tag="ACHKCL_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5761564.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34305..35060)
                     /gene="flgF"
                     /locus_tag="ACHKCL_000523"
     CDS             complement(34305..35060)
                     /gene="flgF"
                     /locus_tag="ACHKCL_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5761565.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35080..36285)
                     /gene="flgE"
                     /locus_tag="ACHKCL_000524"
     CDS             complement(35080..36285)
                     /gene="flgE"
                     /locus_tag="ACHKCL_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5761566.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36310..37005)
                     /gene="flgD"
                     /locus_tag="ACHKCL_000525"
     CDS             complement(36310..37005)
                     /gene="flgD"
                     /locus_tag="ACHKCL_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5761567.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37017..37421)
                     /gene="flgC"
                     /locus_tag="ACHKCL_000526"
     CDS             complement(37017..37421)
                     /gene="flgC"
                     /locus_tag="ACHKCL_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5761568.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37425..37841)
                     /gene="flgB"
                     /locus_tag="ACHKCL_000527"
     CDS             complement(37425..37841)
                     /gene="flgB"
                     /locus_tag="ACHKCL_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5761569.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37996..38655
                     /gene="flgA"
                     /locus_tag="ACHKCL_000528"
     CDS             37996..38655
                     /gene="flgA"
                     /locus_tag="ACHKCL_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5761570.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38731..39024
                     /gene="flgM"
                     /locus_tag="ACHKCL_000529"
     CDS             38731..39024
                     /gene="flgM"
                     /locus_tag="ACHKCL_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5761571.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39029..39445
                     /gene="flgN"
                     /locus_tag="ACHKCL_000530"
     CDS             39029..39445
                     /gene="flgN"
                     /locus_tag="ACHKCL_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5761572.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39485..41020)
                     /gene="murJ"
                     /locus_tag="ACHKCL_000531"
     CDS             complement(39485..41020)
                     /gene="murJ"
                     /locus_tag="ACHKCL_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5761573.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41130..42053)
                     /gene="yceM"
                     /locus_tag="ACHKCL_000532"
     CDS             complement(41130..42053)
                     /gene="yceM"
                     /locus_tag="ACHKCL_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5761574.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42055..42702)
                     /gene="yceH"
                     /locus_tag="ACHKCL_000533"
     CDS             complement(42055..42702)
                     /gene="yceH"
                     /locus_tag="ACHKCL_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5761575.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42713..43297)
                     /gene="rimJ"
                     /locus_tag="ACHKCL_000534"
     CDS             complement(42713..43297)
                     /gene="rimJ"
                     /locus_tag="ACHKCL_000534"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5761576.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43533..44741
                     /gene="mdtH"
                     /locus_tag="ACHKCL_000535"
     CDS             43533..44741
                     /gene="mdtH"
                     /locus_tag="ACHKCL_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5761577.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44805..45452
                     /gene="grxB"
                     /locus_tag="ACHKCL_000536"
     CDS             44805..45452
                     /gene="grxB"
                     /locus_tag="ACHKCL_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5761578.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45586..46146
                     /gene="yceB"
                     /locus_tag="ACHKCL_000537"
     CDS             45586..46146
                     /gene="yceB"
                     /locus_tag="ACHKCL_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5761579.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46252..47298
                     /gene="pyrC"
                     /locus_tag="ACHKCL_000538"
     CDS             46252..47298
                     /gene="pyrC"
                     /locus_tag="ACHKCL_000538"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5761580.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47372..47617
                     /gene="dinI"
                     /locus_tag="ACHKCL_000539"
     CDS             47372..47617
                     /gene="dinI"
                     /locus_tag="ACHKCL_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5761581.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47907..48161
                     /gene="bssS"
                     /locus_tag="ACHKCL_000540"
     CDS             47907..48161
                     /gene="bssS"
                     /locus_tag="ACHKCL_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5761582.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48276..49394
                     /gene="solA"
                     /locus_tag="ACHKCL_000541"
     CDS             48276..49394
                     /gene="solA"
                     /locus_tag="ACHKCL_000541"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5761583.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            49442..49555
                     /locus_tag="ACHKCL_000542"
     CDS             49442..49555
                     /locus_tag="ACHKCL_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5761584.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12013 a        13357 c        12332 g        11942 t
ORIGIN      
        1 tgattatttt tataataacc tttcattcac agtcaccagg tacaacggca tcatggcaac
       61 tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc
      121 cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc
      181 ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa
      241 agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga
      301 agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc
      361 catcgccaac tgctttaccg gcaaagacac cccggcaggc tgcttcatga tcaacaactg
      421 caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat
      481 gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac
      541 ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat
      601 cagcgcacgc gaaggtgcat cgttggaaaa actgatgcag attgccggaa cgactttgcg
      661 tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag
      721 gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccgtta
      781 ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg
      841 ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta
      901 caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta
      961 ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc acgaccacca
     1021 ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga
     1081 tgttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt
     1141 gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccacgt
     1201 tcaaacacgt tcagactggc cactgccgct acgccaagcg cagctgcgac accaatcccg
     1261 atacccgcca acattgattt attcacggga atacctcctt ctttgcagtt atgccaatca
     1321 tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga
     1381 aatacttaag gattcggagg agtctgaagg aatgcctgat gcgacgctta acgcgtctta
     1441 tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc
     1501 agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac
     1561 cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag
     1621 cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt
     1681 acccatcagg ctaccaacgg tggagaagtt cgacagcgat accagcgaac catgatcttt
     1741 atactgataa tttttcagca gcttaccgtt catctgcgcc agaatgttgt tcattgcgca
     1801 agtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg
     1861 gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcag
     1921 ttccaccacc agctggttga tacggttagt ttcaagacca ccgatatctt tcaggaagtc
     1981 tggcgctttg atcccggctg cccataccat cagatcagcc tcaatatatt cgccatcttt
     2041 agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagaa cgcgaacgcc
     2101 aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg caggcaaaat
     2161 acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc
     2221 gtagctgtgc agttgcttga ccgcgttgtg caattcagcg gagagttcta cacccgtcgc
     2281 gccgccgccg acaatcgcaa tgttcacttt gccatttgcg cccaggttgg cggagtattt
     2341 caggaagaga ttcagcatct cctggtgaaa gcgacgcgcc tggtgcgggt tatcgaggaa
     2401 aatgcagttc tctttgacac ctggtgtatt gaaatcgtta gaggtgctac ccagcgccat
     2461 taccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt tctcatcgcg
     2521 cagttctgca atagtgattg ttttcgcgtc acgatcaata tcaatgacgg aacccagctg
     2581 gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc
     2641 aagcgagcca gtcgctactt cgtgcagcag cggtttccac aggtggctgt ggttacgatc
     2701 gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat
     2761 ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg tagtcaacgt
     2821 gaccccctta aatttattaa ccaattgtta attaatggtt attaacatag ctcttaatta
     2881 acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt
     2941 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
     3001 ggtgagatca aaaaacagag tttattgttt attatttgag cgaatttgtg agagtgatat
     3061 agcgtgaggg gtgtatgtag ggcgttcggt aggtctgata agaggcgcat caggcattga
     3121 tgctcgactg tcggatgcgg cgtgaacgcc ttatccgact tacagaattc ggagcgcatc
     3181 aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt
     3241 aatgcgctgc aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac
     3301 aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagcg cttcatcatt
     3361 acgcgacaaa atcaccaggc aacgatcgcg attcttctcg cggaagttgg tcacacactt
     3421 agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg
     3481 gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc
     3541 ccagtatccg cccagaccaa cgccgcaaat caggggacgc tcgtcgacgt tcagttgcaa
     3601 cattttgtcc acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta
     3661 gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc
     3721 cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg
     3781 gttagtgacc tgctttctct tcctgccagc gttcatgtaa ctgattcaga cgggcgctga
     3841 tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca
     3901 aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt
     3961 tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatacc tgcccgcgtt
     4021 cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc
     4081 cgtcaaaacc cagttcctga cgcaaaacgg ttttcagcca gtaggaagaa ccgctcgccg
     4141 gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt
     4201 cgcggattaa cgtgctgaaa accgacatat ctttggcacg aatttccgct tgcgggcgtg
     4261 ggtcgcacgg cgtctctttg tgtgaatctg cagttactgc accgtgtcct gggaagtgtt
     4321 tcccggtcgt tttcattccg gcttcatgca tcccatcaat aaaccgactg gcgattgcca
     4381 gggctttttc tgggtcggca tgataagaac gctcgccaat cgccgcgctg atatgtccta
     4441 catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca
     4501 gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc gcagcaaagg
     4561 attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctggacgcgt ccaccttcct
     4621 gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc accagttcac
     4681 gcaactgggc aggatcatga tagtttcgcg taaagagaat cagccctccc accagcggat
     4741 gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg
     4801 gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc
     4861 ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc
     4921 atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc
     4981 gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacacc
     5041 gccgccagtt ccagcgcgat atcaccatct cctgcatact cccagtcgat gagttttaac
     5101 cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt
     5161 aaaggccgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc
     5221 cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc
     5281 cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc
     5341 ggcaaatacg tttttacctc gccgggcaga tagtcgacta ccatccagtc acggagatat
     5401 aaatgcggct tcggtgcaat gcatgcgggt agttgtgata aagcccgata ctggcgcaag
     5461 aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga
     5521 tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac gggatgaaac
     5581 tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct gcgaaacggc
     5641 acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt
     5701 cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg
     5761 ggcaatgcct atggctttac tacgggtgcc taaactatcc tgcggcgaca aacctaactg
     5821 ttgcttagcc atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag
     5881 cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt
     5941 aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact
     6001 gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc
     6061 agtttgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac
     6121 tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac
     6181 cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa
     6241 ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga
     6301 ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa
     6361 tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt
     6421 aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagagtt gaggatgctg
     6481 aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata
     6541 aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac
     6601 agcccaccag cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta
     6661 cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat
     6721 gtggtaaacc tcttgtccgc catggcggtt ggtgttcatg atcagacgat agccatcttc
     6781 ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg
     6841 ctcatgctca gctgagacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat
     6901 atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac
     6961 gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac
     7021 attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta
     7081 tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa
     7141 aatcgttttg ctgccgtata tatctccatt attcccattt ctgcctgcga atgcgctgaa
     7201 aagacattct ctgtttggtt tacacgacaa atatgaatgc gtatatttct catttgcatt
     7261 tgcatttgca agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc
     7321 atgagcctga cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg
     7381 gataatcaat atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca
     7441 ctattacctt ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt
     7501 tcagccacag ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt
     7561 accaaaatgc agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag
     7621 cggatggaag atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc
     7681 agcaaaagtc aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat
     7741 aactatatgg ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca
     7801 ctttctgata tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg
     7861 accgggacgg gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt
     7921 gaatttaaag gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg
     7981 gatttacaaa gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac
     8041 cagaataacg actcatggct ggaccgctac aatagtgaaa agaccttctt ctccggcatt
     8101 gtcgatgctg atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt
     8161 gatgttaata gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac
     8221 agttacgatc gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac
     8281 aaggtcttta tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc
     8341 acccactctg aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa
     8401 gcggatggta tgctggttgg gccatacagt aattatggac ctggctttga ttatgtcggc
     8461 ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt
     8521 agttatgaat tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa
     8581 aacaatcgtt acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac
     8641 aactttaatg gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat
     8701 accacacata tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
     8761 ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag
     8821 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
     8881 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
     8941 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
     9001 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
     9061 ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc
     9121 agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt
     9181 ggcgcaacgc gctatattgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
     9241 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc
     9301 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc
     9361 ttccgcgccg agcaaggtag ctacgcgttg gtggatctct tcacccgcta ccaggtgacg
     9421 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
     9481 gtggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
     9541 ttctgaataa cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt
     9601 acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta
     9661 ccgaaaatcg cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
     9721 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
     9781 gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt
     9841 tcgctggtac ctgtcgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga
     9901 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg
     9961 acgatcggga acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga
    10021 ccgtgcgaaa acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg
    10081 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg
    10141 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    10201 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    10261 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    10321 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc
    10381 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg
    10441 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    10501 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    10561 gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc
    10621 agcttaatac ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga gataatccct
    10681 ccgagtacgc cagtatccgc caggtgttta gaggcgattt cttcagcagg taaatgcagt
    10741 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcagcc
    10801 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt
    10861 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    10921 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg cagtacggat
    10981 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    11041 attgagagtg ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga
    11101 gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat
    11161 tcagtgccaa tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca
    11221 cattttgaca aaacatatgt cacaatattt caaacgtggt tattcaccgc ccaattttcg
    11281 gcggtgaact ttactcgttt tagtcattaa ttacgagctt taaaaaaact cattcaacgg
    11341 atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc
    11401 tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga
    11461 accatcgcag gttgattctc ttttcctcga tgcggcaccg gcgcaaggta aggtgagtca
    11521 gtttccacca gtaaccgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca
    11581 ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttgccc
    11641 gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct
    11701 tcgcgcagaa ttgccagcgt atcggcgcgg gcgtcacggg tatggacgat aaccggcttg
    11761 ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt
    11821 tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc
    11881 gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca
    11941 caagaaaata cgacgttgtc acgttcgcct accagatccc gcatgtgtaa ataacccggt
    12001 aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg
    12061 tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg
    12121 actaaaaaca tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag
    12181 gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac
    12241 agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg
    12301 agaaagatgg tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc
    12361 agcgccatga tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc
    12421 cggagcttgt tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct
    12481 atatgccaac gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc
    12541 cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga
    12601 cattgtcact tcgcgtgaaa gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta
    12661 atgtaaccga caacgactac gtaacgttgc cagtaaacgt tcaggctcgc gggtagccag
    12721 gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc
    12781 gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta agcgtgcgtg
    12841 ctcattcagc ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt
    12901 ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg
    12961 acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc
    13021 gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc
    13081 tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca
    13141 tctcatgcgt ccaactcctt cacccagcgg gtcacggtag tgcggattgc atccatcacg
    13201 gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc
    13261 agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc
    13321 tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt
    13381 aagtcggggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca
    13441 ataccacgtc ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaataacc
    13501 caggtgccgt tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca
    13561 taaaacatca gaacttcggc tttatcggta atgatttcat cgcctaccga tttgatatcc
    13621 agcaccaggc ttcttaactt ttcggcaagt tgcgtaccac caggttcccg agtgaaaacc
    13681 atgtcgcgga tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg
    13741 cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac
    13801 tttcagataa tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc
    13861 acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt
    13921 cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt
    13981 gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc
    14041 catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat
    14101 aaataccgag gcaacctgat cgcgttcact ggcaatggcg gtttcttttt cgataattga
    14161 tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca
    14221 ggcgctatcg accgctttca ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt
    14281 ggtattggcg gtatacatcc aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt
    14341 ttccagttca agtgcctgcg ctacggtgat gtacttatca tcgctcagcg tgtgcttgat
    14401 atacggggcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg
    14461 cagagggaac tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat
    14521 ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag
    14581 caaccattga aaaacccgtg ggcgattgat gatcttatcg gcataaagct gttcaccaag
    14641 cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt
    14701 gctgtcggca agatagcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac
    14761 cagcaataac aagattatca ataacacttt tttcatgact aattcgggcg ctcacaaagt
    14821 ggggctaaat attcatataa cgttgctgac gaaaaggaga catcgccaca ggcacgtacg
    14881 ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcttcc
    14941 agagaggctt gcacttcgac aagctgataa gaggattgtg ccagcaaacg gatacagaat
    15001 tgtcgcataa tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacgttgccc
    15061 ttccgccaga acaaattagc tgcacagcat tccgtaaccc ccccttcgct gtcaaggacc
    15121 agcgcctcat cagcgtttgt ctgctcaaga tgagagcgga tcaatacttg ctcaagccga
    15181 ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc
    15241 aacgtgatcc cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga
    15301 atccgcgttg ccggtccact gttcaatgtg ctgtaccctc gcccgccact accgcgactg
    15361 atcacgactt tcagtacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca
    15421 agctgaggcc agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg
    15481 atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca
    15541 tcaccaaact gcgttgcccg atcgcttact gacagcgatt cctgcttata accgttaatt
    15601 aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac
    15661 aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa
    15721 tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt
    15781 tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat
    15841 cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg
    15901 ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag
    15961 atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct
    16021 gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct
    16081 gactcgcttc aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc
    16141 cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt
    16201 cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac
    16261 cggcaccatc gcccagtacg aaaccatcac gctctttatc ccacgggcgg ctcgccgctt
    16321 gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca acacccagcg
    16381 gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa
    16441 tacgcgcagc atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc
    16501 ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg
    16561 acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt
    16621 cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt
    16681 tctcttccgt tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat
    16741 attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa
    16801 aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta
    16861 ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga
    16921 caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc
    16981 agggagggaa aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc
    17041 gcctggagat gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg
    17101 tggtgatttt ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca
    17161 ttaccagctc aacggtgtca agagaatccg cgcctaggtc ttcaacgaaa gaagcattgt
    17221 tggtaacttc ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt
    17281 cgatagtgct catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag
    17341 tgtataaaat gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt
    17401 tattttgagg ccttaccccc caataacgca aatcattttg cactaattat tactcataac
    17461 cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc
    17521 cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg
    17581 caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcaccagga
    17641 tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag
    17701 caacaacgtt tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc
    17761 cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa
    17821 ccacagaacc gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg
    17881 cttttgacag acggaaaaca gatgaaaggt tggtttcgat aatatcgttc cactcttcat
    17941 ctttcattcg cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca
    18001 cttcaccaaa ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca
    18061 cattcaacat cagacctttg ccgttagcac ctaaataatc actgatcgcc tgagcgccat
    18121 tttcactggt cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg
    18181 cgcggccaat tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga
    18241 tgttcctctt ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg
    18301 cggtcagggt gtcgacaatg cgtttcgtca ggccagtaag cactttgccc gggccgactt
    18361 catagagatg ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg
    18421 ggttatacaa ctgacgtacc agtgcgtcac gaatggcatc accattggtt tcgcatttca
    18481 catcaacgtt attcacaaca ggaactgttg gtgcgttaaa ggtgattttc gctaattcta
    18541 ctgccagttt gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta
    18601 acggcagagc gcgttttgcg cccgcagctt tacaggcagc gccagcacgc tcaaccgctt
    18661 ctttatgacc ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct
    18721 gaccttctgc agcttcttca cacgcttttc caatagacgc atcatccaga ccgatgattg
    18781 ccgccatagc gcccgttcct tccggtacgg cttcttgcat gaacttgccg cgcatctcaa
    18841 ccagacgcac cgcatcagcg aaatcaatca caccagcgca aaccagcgcg gagtattccc
    18901 ccaggctgtg accggccatc attgccggtg ctttaccgcc atgctgctgc catacgcggt
    18961 acagcgcaac agaggcagtc aacagcgctg gctgggtttg ccaggtttta ttcagttctt
    19021 cagctggccc ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag
    19081 caaacgtttc ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt
    19141 gagaaccctg tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga
    19201 aacgaaccag cgcggagccc caggtgaatc caccgccaaa ggcttcaagc agaaccaact
    19261 gccccggctt gatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg
    19321 cagaggtatt accgtggcga tccagcgtca ccacgacatt gtccatagac ataccgagtt
    19381 ttttcgccgt tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt
    19441 gagaacggtc aagattattc gccgccagcg tctcatcaac gatgtgcgcc agttccgtta
    19501 ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca
    19561 cgcggtcggc gtttggcagc gtcagcaatt caccataact accgtcggca tgcagatggg
    19621 tggaaatgat tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa
    19681 aaataataat agtcccacga tctgttggat cgcaggtgcg cgccagtaca tcggaaccga
    19741 cgaccagagc atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat
    19801 aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca
    19861 tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa
    19921 cgatcaggcc aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt
    19981 caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg
    20041 tacgagtgac aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc
    20101 gcacttgttc gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt
    20161 cacttttcgg ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac
    20221 cgcagagttc cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc
    20281 aggcgagcgg caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc
    20341 gcgaccgcaa aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat
    20401 cctaacagac aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg
    20461 ctcttttgta gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc
    20521 tgagatttca gcagagaaag gaacatcctg acaacacctt ccatcgtctt taatgtgaca
    20581 tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct
    20641 tcaagatagc cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga
    20701 atactatcga gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg
    20761 ggaatttcca ccacctcttc agccaggact gagcccataa tggcaaattg caccaacatt
    20821 gtgctgtcac aatcgacgtt ggcccctaag tcaaggacca ccgttttgcc cttttgctga
    20881 tgtggtaata ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat
    20941 tttgccagcc ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct
    21001 tctttcacca gctccagggc cacgcgcatt gaactcccac gactggcgcg gatagcttgc
    21061 gaaggccggg catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt
    21121 tgttcaaagt cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga
    21181 agagtgagtt gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg
    21241 ccaaaatccc ctcccatgac atctaacgcc agggttagac gtgtcaaggt atcgtcgccg
    21301 cccggtttgg tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga
    21361 cgcgtgatta cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt
    21421 gacgcaggtg tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg
    21481 cgtcatggga acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca
    21541 tggaccttac tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac
    21601 gctttaagct ggctaatacg gcaaatgggt ttggcttttg cgcttcttca ggcagttcac
    21661 caaagaccat gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg
    21721 gcaaggcgag gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt
    21781 taacctcaat cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag
    21841 gactaaaaca atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact
    21901 cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag
    21961 cgaacgacat ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct
    22021 caacctgatc aggagtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg
    22081 gatcgagagt caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg
    22141 tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt
    22201 gcgggttact gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag
    22261 cgcatagttt aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa
    22321 acttatttta gcctccacct cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc
    22381 ttttgaatgt gcagcacccg aggtcgacga aactccacac agcgacgaat caccgcgaca
    22441 gttggtgctt cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca
    22501 tttaattatt ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt
    22561 aacggaagaa aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcactttcta
    22621 tactggactg gcgttgttta attcggcgaa tgggcatctg caaacagaag tggagccttt
    22681 tgacgtccat ttccgtcacc tgagcgaggc ggagattgat aattacgtgc gtaaagagca
    22741 tcccctgcac tgcgcgggta gctttaagag tgaaggattt ggcattactc tgtttgagcg
    22801 tttagagggg cgtgatccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat
    22861 gttacgtcgg gaagggaaaa acccgctgat gggatagttt tttgcgccgg atgcggttac
    22921 gtcgcatccg gcaatcggtg ctggcggcct gatgcggcgt aaacgcctta tcccattatc
    22981 atgcttatat gctcgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacca
    23041 tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac
    23101 gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca
    23161 aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacctgcata ctgtgtatgc
    23221 acacggatct gatgagtgcg ccctgttacc ggactacaac gcaccagggt ggcaaatgca
    23281 tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact cacacgcacg
    23341 atacgttcgc cgctttgcag aatatttttc aataacggcg cctgaacgct cttcacatgc
    23401 gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt ttcacgtaat
    23461 tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaggtg
    23521 tcccggtcaa gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa
    23581 ccttcaataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta
    23641 ttcagcacca ggatgtgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt
    23701 tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tggaatacgc
    23761 acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact
    23821 tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc
    23881 aaaaagttgt cgatacgttg ccccgcttcg tcggcggtga tagcaacaat ttttacggat
    23941 ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt
    24001 tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat
    24061 ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg
    24121 gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc
    24181 atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg
    24241 cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc
    24301 ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc
    24361 gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata
    24421 accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt
    24481 actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat
    24541 gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct
    24601 gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg
    24661 taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg
    24721 tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc
    24781 tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat
    24841 cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg
    24901 tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc gccgtatcga
    24961 aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat
    25021 ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc aatgggattt
    25081 aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggcccc
    25141 gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca
    25201 ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac gtcagcatat
    25261 cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc
    25321 gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg
    25381 tctgccatcc ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat
    25441 caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta acactaacct
    25501 cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt
    25561 tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg
    25621 tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct
    25681 gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagtc atcacgtttg
    25741 tccgcgttgt tctggtactg gcaccgtgcg tgacaacgaa tcgctgtcgc tctctattct
    25801 gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc
    25861 tgtgccaatc gcttcttacc tgctgaatga aaaacgttct gcggtaaatg ccattgaaac
    25921 tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa ccccgcacta
    25981 ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca tgctgccgaa
    26041 gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc
    26101 ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcgc caacgccagc
    26161 tgaacctgcc gcgcctgttg tagccccagc accgaaagct gcaccggcaa caccagcaac
    26221 tcctgcacaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg
    26281 tggtgaagaa accaaaccga ccgagcaacc agcaccgaaa gcagaagcga aaccggaacg
    26341 tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga
    26401 cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg
    26461 ccaagcacag cagcagactg ccgagacgcg tgagggccgt cagcaggctg aggtaacgga
    26521 aaaagcgcgt accgccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa
    26581 cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt
    26641 tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca
    26701 gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaaacggtag tcgcaccggt
    26761 agctgaagaa actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc cacgcacaga
    26821 actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa
    26881 caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca
    26941 cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccaatc
    27001 gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat
    27061 ccgctatccg attgtacgtc cacaagatgt acaggttgaa gagcagcgcg aacaggaaga
    27121 agtgcaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aaccggttgt
    27181 tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga
    27241 accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga
    27301 acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg cagaacacgc
    27361 agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc
    27421 tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga
    27481 agtcgcagca gaagttgaaa cggtaaccgc ggttaaacca gagatcaccg ttgagcataa
    27541 ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg
    27601 tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca
    27661 tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    27721 agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcatct ttgaatcaac
    27781 atattaaaat tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat
    27841 gcccgccccg gcgatccctg agcgggcaga caaaacatac ccagtttcat gatatgtttt
    27901 aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt
    27961 atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc
    28021 cacatcaacc agatcgctca tctgctgcgt ttgccctaat gcgcgatcgc tacctaatga
    28081 atccagcgac tccagttcgt tcagctgcgt gcctaattcc gcgcgaacgg tcaggacatt
    28141 gttcagcgag ttttccagtc cgcggttggt tttatctaac gccgcagcgg cggtttcttt
    28201 atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc
    28261 aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact
    28321 gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg
    28381 cttaatactt tctgttccgc cgacataatc accgttaacc tcactaaacg gcgcagtctc
    28441 tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg ccagattcag
    28501 caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat tgtcactcaa
    28561 ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcgg tggtgacctg
    28621 gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta
    28681 ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc
    28741 gtctgaaggg ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc
    28801 tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg
    28861 cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc
    28921 agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca
    28981 tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct
    29041 tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag
    29101 gcataagcgt cgttaaagga tttcgctccg cccaccgttt tactgttgct ttgcagatcc
    29161 agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgct
    29221 attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc
    29281 agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat
    29341 gccactttac cgttggcatc tggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg
    29401 acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac cgcggaggca
    29461 tcagttaccg tggcaccgat cgcaacgtta ccgttgtttt tagtgttttg cagaaccgcg
    29521 ggcttaccga tagcaaagaa atcttcaccc tcatcgccgt tggcatcaaa tccggctttg
    29581 tgttggctgt tgaaagcctc agcaaatgcc agcgccagtt gtccaagcgt attacgcgtc
    29641 tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat
    29701 ttctccggga tctcaatatt gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa
    29761 gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag
    29821 taaccattgg ccatcgtgat gttataagtg ccgccatcct gaacgctgac ttctacacca
    29881 acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac
    29941 gcccctgccc ccacgcctgt caggcgggag atttgatcgt tcaggctggc gatttgttta
    30001 gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg
    30061 tcgcgaagat actgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc
    30121 agcgcctggc gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag
    30181 aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc
    30241 gacatctgct catagcgggc agtcaggccg ctactttgcg tctgcgccgc acgtaactgg
    30301 ttggtaataa acgcatcata ctcacgctgc acaccagaaa cgtagacacc attaccaacc
    30361 cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat
    30421 ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg
    30481 ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca
    30541 attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa
    30601 tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tgaatcatgt
    30661 tggtgagttt acgggcatag tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg
    30721 ccccctgctc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca
    30781 gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta
    30841 ctttcttcgc ttcgccattt tcatattcag tcgtggtgat ttcagtgact ggccctttcc
    30901 agttgccaga ggctttgaca ccaaacaggt tatagctcgg ctcgccgttt tcgcggcgga
    30961 tttgccgttg cccccagcca gattccagcg ccgcctgagc gaggatcaaa tgatgtggca
    31021 caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctttac
    31081 tgtcacctgg cagcgaatca tcgtagttac gtggcacggc cttttgcacc agctgcgaaa
    31141 gcgcctgatt ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg
    31201 tggactcctc tggcaatggt tgttctggcg tcatctgctt aaccatcatc tctgcaagcc
    31261 ccagaccttt gcccgtcgtc atctgttggg caatctgctg gtcatacata ctggtataca
    31321 ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa cgcgtcgcgc atgcttttca
    31381 acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg
    31441 gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg
    31501 ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc
    31561 actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag
    31621 cgcgcgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct
    31681 ctggcgtaag tcgatctgcg tttgtggcgt taccacggtc tgtccgccac caaacggtgt
    31741 atctggttgg ctgacattgg cctgacgatt gactgttact gagagatttc cctgcgctat
    31801 cgcgcagctg tcgagagtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat
    31861 cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa
    31921 acggacctgg gaactgttgc cactcggtac gcgcacctga atagtccgcg catccaacgc
    31981 ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct
    32041 gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggtaa
    32101 ttcacgttca ataactgcac cattggtgat ccgtccaccg ttcagttggt tcacctgaac
    32161 actgctaccg ccagcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc
    32221 atacacctga ctgtcaacgc ccttaagcgg agtcatcaac aatgtgccgc cacgcaggct
    32281 tttggcattt cccatggaag aaaccaccac gtcaatggtt tgcccctgac gtccaaacgg
    32341 tggaagtgac gccgtcacca ttaccgcagc aacgtttttt agctgcatat tggtgcccgt
    32401 cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt
    32461 ctgggttgtc tggtcaccgg tgccatccag ccccaccacc agaccatagc caatcagtga
    32521 gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt
    32581 cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt
    32641 ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag
    32701 ccattgccta cgtattcaat gcgcgcatcc gccacctgag tagacggtac ggtattgctg
    32761 ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa taaattcggt accctgatta
    32821 atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc
    32881 gtcaccgtca acgtgccgct aaaggtatta ctggcattgg ccccgccttt tccgttgaac
    32941 gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag
    33001 cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag
    33061 cttttgctgg cgctaacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt
    33121 ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa
    33181 atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc
    33241 accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa
    33301 atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcaataaacc
    33361 ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt
    33421 ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag
    33481 ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc
    33541 gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt
    33601 ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg gtgagattga gctgcccaac
    33661 ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt
    33721 gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt
    33781 caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc
    33841 atctggcaac atcacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttgtt
    33901 ggtctgcgac aggttgcctt ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt
    33961 gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg
    34021 aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact
    34081 gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt
    34141 tttggcgatc cataatgaac tgatcataaa atgtcctgta gtggattgct gacaagcaga
    34201 gcagaagcac gcggcggtgc gaatttcccc tcaccctaac cctctcccca aaggggcgag
    34261 ggaacagtcc gctcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc
    34321 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    34381 aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    34441 tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg tccacgcgtg
    34501 gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc
    34561 gtggctttca ccagtttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga
    34621 ttcagcgccg agattgttcc atcggcagct atcgtgattt ccgccccttc cggcacagca
    34681 attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca
    34741 acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccaac
    34801 cagccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc
    34861 gttatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct
    34921 tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg
    34981 gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgcg
    35041 gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt
    35101 gttgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc
    35161 aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca acgcaccgtt
    35221 ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg
    35281 cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc
    35341 cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc
    35401 gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct gggtggttgc
    35461 cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt
    35521 ggcgggttct gcgccgttaa ttgcgccggt ggtaatattc tctgttggat ttgaggtcag
    35581 aacgccattg gcattaaaca ccagcttcat tgcaggctcg gctgtacctg ttggatcact
    35641 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat
    35701 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    35761 cgcattgctg gcatcaaatg cgttaacaga gggaagcgga tcgctggaat tcaggttgat
    35821 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatgtt
    35881 agtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag
    35941 ctgtaaacct tgcatattca ccaggttacg attttcatcc agcttaaatt gtccgttacg
    36001 gctgtagaac accgaaccgt tgctatctac cagacggaaa aaaccgttct ggctgatagc
    36061 aacgtccaga ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    36121 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    36181 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    36241 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    36301 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    36361 atccagcgta ttaccgttgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    36421 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacgttgt aagaaccgtt
    36481 cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt
    36541 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    36601 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    36661 ttcttcactg ccggttcccg caagaacagt ggtgccgggg atcatcacgc cgtgaccgat
    36721 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    36781 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    36841 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag
    36901 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    36961 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    37021 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    37081 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ctccgacaac atcaacgttc
    37141 ggcattttta cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg
    37201 tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggt
    37261 gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc aggaccggtc
    37321 acgctatcag cattcgccag attactggcc gccacgttca ggcgctggga ctgggcagtt
    37381 aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt
    37441 aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc
    37501 aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacggtatt accgtcaagc
    37561 gaaggctggt ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc
    37621 ggaatgtgtt gcgttgaggt catcgtcaga gcaaccacac tggttgcatt ccgtccacgt
    37681 tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta
    37741 tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc
    37801 tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca
    37861 aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa
    37921 tgggtcgcta tttatgccgt tgatggtcat tgtggacagg tacaattcac gttgtagaaa
    37981 tggctggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc gatactgttc
    38041 agtccgttaa gtgcggcgag caatctcaca tcgcaattgc aaaccttttt tagctcccag
    38101 ctcgcaggga taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg
    38161 ccgtgcgagc agccattgct ttcgatgagc aataattccc gcttgtgggg caatgtgaat
    38221 gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga
    38281 aattatgtgg ttgccgcgat gcccattgtg cgaggaggaa agctgggagc tggcaatgtc
    38341 aaactgaaac gcggacggct ggataccttg ccaccgcgta cggtgctgga tatcaatcaa
    38401 cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag
    38461 tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagcggtgat
    38521 gggtttagcg ccaacgcaga aggtcaggcg ctaaacaatg cagccgtcgc acagaatgcg
    38581 cgggtgcgca tggtatctgg acaggtagtc agcggcgttg ttgatgcaga tgggaatatt
    38641 cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat
    38701 gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag
    38761 cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa cacccgagcg
    38821 gcaaaaacaa cagcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg
    38881 atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt
    38941 cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagcg
    39001 cagcaagact tgcagagtaa ttaatcgtat gacgcgtctt gcagagatcc tcgaccagat
    39061 gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat
    39121 ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc
    39181 aacgttggat tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga
    39241 tattagtcaa cgctggcagg aaattgctgt gaaaacgcag caactacgcc aaatgaatca
    39301 acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt
    39361 gaaaccgcat caggaaccga cgctatatgg ggcgaacggt cagacctcaa caacccatcg
    39421 cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatgc
    39481 attgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtacc gccagtgcag
    39541 cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagcaaa cgccagggca
    39601 tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca
    39661 ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccaggttgc ggggtaaaga
    39721 ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa
    39781 tagaaagtga cagcccggca tgtttcaacg gaccgataaa cgccaggttc atcaattgcg
    39841 ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa
    39901 agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta
    39961 acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa
    40021 cggttaacgg accggaaaga atgcccaacg caaccgcact cggcaacgcc aacaggaaac
    40081 aaagacgcag tccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac
    40141 ttttcgacag cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa
    40201 actccattaa gcggtcggcg taatacatcc atgacaccga accggaagca agaaacgagg
    40261 caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc
    40321 ccatctgttt caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca
    40381 gcatgccgat cttcttcaga tgcggtagct gatacaccag ctgcaggacg ccgccgaccg
    40441 tcacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca aacagcgcga
    40501 aaccaatcat gctgatgtta agcagtgttg gagcaaacgc cggaatcgag aagcggttcc
    40561 acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg
    40621 taatctttag tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg
    40681 cggtcaccat gatcacccac ggtgcggcga gcatgccagc gaccgtcaca accgccagcg
    40741 caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct
    40801 tacttttata ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga
    40861 tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg
    40921 cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg
    40981 tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc
    41041 acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg
    41101 tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg
    41161 tcaacgatac gttgcgccag cactgcctgt tcgccggcgg tttgcggaac tgtctgattt
    41221 tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagcgtgctc
    41281 tgccaaccag gaatcggttt attcactaca ccctgcccac gctcctcacg ccattcgcgc
    41341 atttccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc acgctgactt
    41401 ccggcacggc gatgcataca agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg
    41461 gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag agaggctttg
    41521 ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa
    41581 taaagatcgt gtggcccgac gctattgcta cgatgtttat ccattcttag cgaggctgcg
    41641 gtggcgagtt gcgtttttaa ctcacagtag agtggtgcga aacgacggtt aaaaccgacc
    41701 atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt
    41761 tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg
    41821 tcaaagtgac tggcggtgct ggaatgtaca aaaaccgcat cgcaactggc ggcaaggctg
    41881 gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc tttcgcgcgc
    41941 gtaggcgacc aggctccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac
    42001 gctttttgcg caatgccgcc taatcccact acgccgatac gtaatttttt cactttaatc
    42061 tcccaggtgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag
    42121 ggcttcgacg cgggcctgta aatcaccgtc aaccgcgttc gacatatccg ttaccgccgg
    42181 ctgatcctct acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc
    42241 gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcaa
    42301 ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc
    42361 cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag
    42421 tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc
    42481 aaaaccgctc actgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac
    42541 ttcagattca ctcagattca tgaccggttc acggttcgtt ttctgattac aggccgtgac
    42601 tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt ccagcaagca
    42661 accaatcaca cgagcttcca gggcagtaag ttgatatttc atgagattct ccttagcggc
    42721 cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa
    42781 tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac
    42841 cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct
    42901 gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc tgccattttt
    42961 gcccaatcga ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat
    43021 tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac
    43081 cttgtttatg aaattcgtta atcatcccca gcctggcctg ccagcctgat ggataacagt
    43141 ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat
    43201 ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta
    43261 agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa
    43321 cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca
    43381 aaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt
    43441 tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga
    43501 aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc
    43561 tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct
    43621 tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta
    43681 ttgccctcgg cctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg
    43741 ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt gccgccggat
    43801 tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg
    43861 gactcggtgg cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc
    43921 cacaacagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca
    43981 ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag
    44041 gggccgtttt gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac
    44101 tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt
    44161 ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga
    44221 tgctgccaat tatggtcaac gatgtggctg gcgcgccctc tgccgttaaa tggatgtatg
    44281 ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc
    44341 attttcgtct ggaacaccgg ttgatggctg ggctgttgat aatgtcatta agcatgatgc
    44401 cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg
    44461 ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag
    44521 ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc gctattggtt
    44581 atatcggtgg cggctggctg tttgacctgg gcaaatcggt gcaccagcca gagcttccgt
    44641 ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc
    44701 agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac
    44761 tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt
    44821 acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg
    44881 tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa
    44941 aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc atggatatcg
    45001 ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca
    45061 ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg
    45121 ccaaatcggc atttgatgag ttttctactc ccgccgcgcg caaatatttc gtcgacaaga
    45181 aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga
    45241 atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg
    45301 gcgaactttc ggaagatgat attcagctat tcccgctact gcgtaatctg acgctggtag
    45361 ccggaattaa ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac
    45421 aaatcaattt gttatcatca atggcgattt aatcctgact cgccgcggca gcctgtattc
    45481 gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt gacacatgct
    45541 gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta
    45601 tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac
    45661 accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat
    45721 attggtttac ccggtgtggc tgacgcccat attgttctga caaacctgac cagccaaatt
    45781 ggtcgcgaag agccgaataa ggttgcccta accggagacg ccaatctgga catgaactcc
    45841 ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat
    45901 aaagaaaaag gcgcgatctt cctgaaagag atggaggtgg tcgatgcgac ggtacaaccg
    45961 gaaaaaatgc aaacggtgat gcaaacgttg cttccctatc tgaaccaggc attacgcaat
    46021 tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg
    46081 gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccatttact
    46141 gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc
    46201 ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca
    46261 ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc
    46321 gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg
    46381 cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg ccaacgtatt
    46441 cttcacgccg tacctgccgg gcacgatttc accccattga tgacctgtta tttaacagat
    46501 tcgctggatc ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa
    46561 ctttacccgg caaacgcaac cactaactcc agccacggcg tgacgtcagt tgacgcaatc
    46621 atgccggtat tagagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg
    46681 acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa
    46741 cctctgcgcc agcgcctgac cgcgctgaaa gtcgtttttg agcacatcac caccaaagac
    46801 gcagccgact atgtccgtga cggaaatgaa cggctggctg ctaccatcac tccgcaacat
    46861 ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta
    46921 cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactagtcgc cagcggtttt
    46981 aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa agagagcagc
    47041 tgcggctgcg cgggctgctt caacgcccca accgcgctgg gaagttacgc taccgtcttt
    47101 gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc
    47161 tatgggttac cggtcaacga cacattcatc gaactggtac gtgaagagca tcaggttgct
    47221 gaaagcatcg cactgactga tgacacgctg gtgccattcc tcgccgggga aacggtacgc
    47281 tggtctgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt ttacctgtat
    47341 aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac
    47401 ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca
    47461 gtatgcgttt cctgataatg aaggccacgt atcagtacgt tatgccgcag cgaataattt
    47521 atcggttatt ggcgcgacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac
    47581 gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt ttgtttttgc
    47641 cgggtcgccc cggctttttt attttacttg cgaattatga gtacaaaatt tctacacttc
    47701 ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt tattgttcat
    47761 ataaatctta aaagcagaaa tttatctgtt ccccccttta aaaattcggt gattaccctt
    47821 atttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga ataacgttgt
    47881 ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt cagactcatc
    47941 cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg ttgcgtttgc
    48001 actaccagac cccaaataag tccgagcagg aagggactga agttggtcag acgctctggt
    48061 taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc gccaaaattg
    48121 aatccggtga tttcccggta aacgagtatc ggcgtcatta acatgacatg accatccacc
    48181 caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca ttcagttaat
    48241 tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc atcattattg
    48301 gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc
    48361 ttatgaccga cgcccatatg ccaccacatc aacacggcag ccaccacggc gatacgcgat
    48421 taattcgaca tgcttatggt gaaggcgaaa agtatgtccc gctggtcctc cgcgcacaaa
    48481 cgctgtggga tgacctctcc cgccacaacg aagatgatcc catttttgta cgctctggtg
    48541 tcattaacct tggcccggct gactccgcat ttctcgccaa cgtcgcccac agcgccgaac
    48601 agtggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc tggccagaaa
    48661 tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggtttt ttgcgcagcg
    48721 aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg caactgttca
    48781 actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa acggctgacg
    48841 gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc
    48901 cggagctgcc tgtccagccc gtacgtaaag tatttgcctg gtatcaggcc gatggccgct
    48961 atagcgtgaa gaataaattc ccggcgttta ccggtgaact gcccaatggc gatcaatatt
    49021 atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga ggccaggtta
    49081 tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt tcggaagcct
    49141 tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc gctgcctgca
    49201 cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc cacgataata
    49261 cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa
    49321 tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca ttcagccttt
    49381 cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac aggagtattt
    49441 aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga tgctatatct
    49501 ttttctctgg ggattgttgg caattatgga tctgatctat gtgttttatt tttagttttc
    49561 cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga aaaattgaat
    49621 gagattattc ctgcttctac agca
//
LOCUS       DAZFKY010000010        49192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFKY010000010 DAZFKY010000000
VERSION     DAZFKY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            92..688
                     /locus_tag="ACHKCL_000543"
     CDS             92..688
                     /locus_tag="ACHKCL_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761585.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            775..1500
                     /locus_tag="ACHKCL_000544"
     CDS             775..1500
                     /locus_tag="ACHKCL_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5761586.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1542..4142
                     /locus_tag="ACHKCL_000545"
     CDS             1542..4142
                     /locus_tag="ACHKCL_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO5761587.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4156..4704
                     /locus_tag="ACHKCL_000546"
     CDS             4156..4704
                     /locus_tag="ACHKCL_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761588.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4719..5324
                     /locus_tag="ACHKCL_000547"
     CDS             4719..5324
                     /locus_tag="ACHKCL_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761589.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5345..5935
                     /locus_tag="ACHKCL_000548"
     CDS             5345..5935
                     /locus_tag="ACHKCL_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5761590.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5947..7056
                     /locus_tag="ACHKCL_000549"
     CDS             5947..7056
                     /locus_tag="ACHKCL_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761591.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7191..7985
                     /gene="panB"
                     /locus_tag="ACHKCL_000550"
     CDS             7191..7985
                     /gene="panB"
                     /locus_tag="ACHKCL_000550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5761592.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7997..8848
                     /gene="panC"
                     /locus_tag="ACHKCL_000551"
     CDS             7997..8848
                     /gene="panC"
                     /locus_tag="ACHKCL_000551"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5761593.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9147..10043)
                     /gene="rpnC"
                     /locus_tag="ACHKCL_000552"
     CDS             complement(9147..10043)
                     /gene="rpnC"
                     /locus_tag="ACHKCL_000552"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO5761594.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10317..10697
                     /gene="panD"
                     /locus_tag="ACHKCL_000553"
     CDS             10317..10697
                     /gene="panD"
                     /locus_tag="ACHKCL_000553"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5761595.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10701..11930)
                     /gene="yadE"
                     /locus_tag="ACHKCL_000554"
     CDS             complement(10701..11930)
                     /gene="yadE"
                     /locus_tag="ACHKCL_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5761596.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11994..12434)
                     /gene="yadI"
                     /locus_tag="ACHKCL_000555"
     CDS             complement(11994..12434)
                     /gene="yadI"
                     /locus_tag="ACHKCL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5761597.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12539..13309)
                     /gene="yadH"
                     /locus_tag="ACHKCL_000556"
     CDS             complement(12539..13309)
                     /gene="yadH"
                     /locus_tag="ACHKCL_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5761598.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13306..14232)
                     /gene="yadG"
                     /locus_tag="ACHKCL_000557"
     CDS             complement(13306..14232)
                     /gene="yadG"
                     /locus_tag="ACHKCL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5761599.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14341..15003
                     /gene="can"
                     /locus_tag="ACHKCL_000558"
     CDS             14341..15003
                     /gene="can"
                     /locus_tag="ACHKCL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5761600.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15044..15580)
                     /gene="hpt"
                     /locus_tag="ACHKCL_000559"
     CDS             complement(15044..15580)
                     /gene="hpt"
                     /locus_tag="ACHKCL_000559"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5761601.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15786..18176
                     /gene="gcd"
                     /locus_tag="ACHKCL_000560"
     CDS             15786..18176
                     /gene="gcd"
                     /locus_tag="ACHKCL_000560"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5761602.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18378..19928)
                     /gene="cueO"
                     /locus_tag="ACHKCL_000561"
     CDS             complement(18378..19928)
                     /gene="cueO"
                     /locus_tag="ACHKCL_000561"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5761603.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMVGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20094..20441
                     /gene="yacC"
                     /locus_tag="ACHKCL_000562"
     CDS             20094..20441
                     /gene="yacC"
                     /locus_tag="ACHKCL_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5761604.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20547..21413
                     /gene="speE"
                     /locus_tag="ACHKCL_000563"
     CDS             20547..21413
                     /gene="speE"
                     /locus_tag="ACHKCL_000563"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5761605.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21429..22223
                     /gene="speD"
                     /locus_tag="ACHKCL_000564"
     CDS             21429..22223
                     /gene="speD"
                     /locus_tag="ACHKCL_000564"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5761606.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22261..22623)
                     /gene="yacL"
                     /locus_tag="ACHKCL_000565"
     CDS             complement(22261..22623)
                     /gene="yacL"
                     /locus_tag="ACHKCL_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5761607.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22799..25396)
                     /gene="acnB"
                     /locus_tag="ACHKCL_000566"
     CDS             complement(22799..25396)
                     /gene="acnB"
                     /locus_tag="ACHKCL_000566"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5761608.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25751..27604
                     /gene="yacH"
                     /locus_tag="ACHKCL_000567"
     CDS             25751..27604
                     /gene="yacH"
                     /locus_tag="ACHKCL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5761609.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27675..29099)
                     /gene="lpdA"
                     /locus_tag="ACHKCL_000568"
     CDS             complement(27675..29099)
                     /gene="lpdA"
                     /locus_tag="ACHKCL_000568"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5761610.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29307..31199)
                     /gene="aceF"
                     /locus_tag="ACHKCL_000569"
     CDS             complement(29307..31199)
                     /gene="aceF"
                     /locus_tag="ACHKCL_000569"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5761611.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31214..33877)
                     /gene="aceE"
                     /locus_tag="ACHKCL_000570"
     CDS             complement(31214..33877)
                     /gene="aceE"
                     /locus_tag="ACHKCL_000570"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5761612.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34038..34802)
                     /gene="pdhR"
                     /locus_tag="ACHKCL_000571"
     CDS             complement(34038..34802)
                     /gene="pdhR"
                     /locus_tag="ACHKCL_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5761613.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35343..36716
                     /gene="aroP"
                     /locus_tag="ACHKCL_000572"
     CDS             35343..36716
                     /gene="aroP"
                     /locus_tag="ACHKCL_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5761614.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36759..37613)
                     /gene="ampE"
                     /locus_tag="ACHKCL_000573"
     CDS             complement(36759..37613)
                     /gene="ampE"
                     /locus_tag="ACHKCL_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5761615.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37610..38161)
                     /gene="ampD"
                     /locus_tag="ACHKCL_000574"
     CDS             complement(37610..38161)
                     /gene="ampD"
                     /locus_tag="ACHKCL_000574"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5761616.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            38249..39142
                     /gene="nadC"
                     /locus_tag="ACHKCL_000575"
     CDS             38249..39142
                     /gene="nadC"
                     /locus_tag="ACHKCL_000575"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5761617.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39345..39785
                     /gene="ppdD"
                     /locus_tag="ACHKCL_000576"
     CDS             39345..39785
                     /gene="ppdD"
                     /locus_tag="ACHKCL_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5761618.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            39795..41180
                     /gene="gspE"
                     /locus_tag="ACHKCL_000577"
     CDS             39795..41180
                     /gene="gspE"
                     /locus_tag="ACHKCL_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5761619.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41170..42372
                     /gene="hofC"
                     /locus_tag="ACHKCL_000578"
     CDS             41170..42372
                     /gene="hofC"
                     /locus_tag="ACHKCL_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5761620.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42407..43450)
                     /gene="guaC"
                     /locus_tag="ACHKCL_000579"
     CDS             complement(42407..43450)
                     /gene="guaC"
                     /locus_tag="ACHKCL_000579"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5761621.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43606..43650)
                     /locus_tag="ACHKCL_000580"
     CDS             complement(43606..43650)
                     /locus_tag="ACHKCL_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761622.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43675..44295
                     /gene="coaE"
                     /locus_tag="ACHKCL_000581"
     CDS             43675..44295
                     /gene="coaE"
                     /locus_tag="ACHKCL_000581"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5761623.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44295..45038
                     /gene="zapD"
                     /locus_tag="ACHKCL_000582"
     CDS             44295..45038
                     /gene="zapD"
                     /locus_tag="ACHKCL_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5761624.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45048..45245
                     /gene="yacG"
                     /locus_tag="ACHKCL_000583"
     CDS             45048..45245
                     /gene="yacG"
                     /locus_tag="ACHKCL_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5761625.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45336..45734)
                     /gene="mutT"
                     /locus_tag="ACHKCL_000584"
     CDS             complement(45336..45734)
                     /gene="mutT"
                     /locus_tag="ACHKCL_000584"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5761626.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(45794..48499)
                     /gene="secA"
                     /locus_tag="ACHKCL_000585"
     CDS             complement(45794..48499)
                     /gene="secA"
                     /locus_tag="ACHKCL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5761627.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48561..49073)
                     /gene="secM"
                     /locus_tag="ACHKCL_000586"
     CDS             complement(48561..49073)
                     /gene="secM"
                     /locus_tag="ACHKCL_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5761628.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
BASE COUNT        12061 a        12974 c        12166 g        11991 t
ORIGIN      
        1 tgataattca cccctattac atcctccaaa aactttgagt aatatccgct cgcaatatta
       61 atttcatata atttttgtaa ggatgcatgt aatgaaaaaa gcacttctcg cagccgctct
      121 ggttatggct tctggttccg ccctggctgt agatggtggt catatcgact ttaacggtat
      181 ggtacagtcc ggtacctgta aagtgggtgt ggtagatact ggtatgcata gcgttaccac
      241 tgatggcgtg gttaccctgg atactgcgaa tgttactgat acttttgctg aagttagcgc
      301 aactgctgtc ggtttactgc cgaaagagtt catgatttct gttgagtgtg atccaggtgc
      361 tccgaagaat gctgagttaa ctatgggttc tgcaagttac gcgaacacca gcggtaccct
      421 gaataacaat atgaacatca ctgttaacgg tattgcaccg gctcagaacg taaacattgc
      481 agttcataac atgaaaaaca aagctggcgc tgctgaaatt aagcaggtcc atatgaacaa
      541 ctcttctgaa gttcaggaac tgacattaga cgcagaaggt aaaggccagt acgtatttaa
      601 cgcatcttac gttaaagcac cgaacagccc ggctgtaact gctggtcatg taaccactaa
      661 cgcgctgtac accgttgctt ataagtaatt gatggtgtgt tgagcataag gtttgccagc
      721 aggcaaacct tattttcgcg aatttaatct ttctacagac agcaaggagt gcttatgttt
      781 cccttggtta aaaaaacggt tagtgcatta tttgtgagta ctctgttagc cagtgcacca
      841 gcattcgctg acattattat ttccggaaca cgtatcattt ataacgctga taaaaaagat
      901 gttaacgttc gtctggaaaa taaagggaat cgccccttac tgattcagaa ctggttggat
      961 acgggcgatg acaatgccga tcccagccag atcaaagtgc cgtttaccgc gaccccacca
     1021 gtttcacgcg tggagccaaa acgcggtcaa accgtaaaag tgatgtatac cggtgcaaca
     1081 gccctgccga aagaccgtga aagcgtctac tggtttaacg tgctggaagt gccgcctaag
     1141 ccgaaagatg ctgaggctga caaaaacttg ttacagctgg ctttccgcac acgcatcaaa
     1201 ctcttttatc gcccgagtgg gttacaggga gaaccggctg aagcaccggc gaaaatcacc
     1261 tggaaattaa acaacgctca gttacaggca aataacccaa cgccatatta cgtgtccttt
     1321 aatgaggtca agctggaaag cggcggcaaa acctacaacg tcaattccag catggtcacg
     1381 ccgttcagcc aggcaagctt tggcgtaacc agtctgccag gaagcgtgtc atccggaaaa
     1441 gtggtcttta aagcgatcaa cgactttggc ggcaacattg atggttccgc aacattctaa
     1501 aaaataaacg gattcttttt aacatcgcca ggaatgcaac cttgtaccag tttactcatc
     1561 aaaaaagccg tatcccgaaa aaaacgctac ttgcggcctg ttgtgccctg ttttatagca
     1621 gcaacggtgc tgcggcggac accgtggaat atgacagttc ctttttaatg ggaactggcg
     1681 catcaacgat tgatgttaaa cgttatgctc aaggcaaccc gacaccgccg ggtctctata
     1741 atgtccgcgt atttgtaaac ggtcaggcga cttccagctt agaaattccg tttgtggata
     1801 ttggcgaaaa cagtgcggcg gcctgtctta cccataaaaa cctggcgcaa cttcacatta
     1861 agcaacctga acagcctgtc actttactcg ccagagaagg tgaagaagag gattgtctgg
     1921 atctggcaaa gtcatacgaa aaggcggatg tgtgctttga cggtagtgac cagtttctcg
     1981 atctgacgat ccctcaggcc tatgttctga aaagctatgg cggctacgtt gacccttctt
     2041 tatgggaatc gggaattaac gctgccacac tggcatatac cctgaacgcg tatcacacaa
     2101 gttcagataa cgacaatagt gacagcgtct atggcgcgtt caactcaggt atcaatttag
     2161 gagcctggca ctttcgtgcg cgcggtaact ataactggac aacagataac ggcagcgatt
     2221 tcgatttcca ggatcgttac ttacagcgtg acattccggc aatccgttcc cagataatta
     2281 tgggtgatgc ctataccacc ggtgaaacgt ttgactctgt caacgtccgt ggtgttcgcc
     2341 tgtacagcga cagccgtatg ctgccttcgg cgctggccag ttacgctccg accatccgcg
     2401 gtgtagcaaa ctccaacgcc aaagtcaccg tgacgcaaag cggatataaa atttatgaaa
     2461 ccaccgttcc gcccggtgaa tttgttatag acgacattag cccttccggc tttggtagcg
     2521 aactggtcgt gaccattgaa gaagcggatg gttccaaacg cacctttacg caacccttct
     2581 cgtcggttgt acaaatgcaa cgtcctggtg tgggccgttg ggatttcagc gcgggtaaag
     2641 tcattgatga cagtctgcga tccgaaccca atatggggca agcctcttat tactatggtc
     2701 tgaataacct cttcacgggt tataccggca ttcagttcac cgataataac tatcttgccg
     2761 ggctgttagg tgtgggtatc aacaccagca tcggcgcctt tgcggtagac gttacccatt
     2821 cccgtgctga aattccggat gataaaacct accaggggca aagttatcgc gtgacctgga
     2881 acaaactttt ccaggatacc gggacatcat ttaacctcgc ggcgtaccgc tattccaccc
     2941 aggattacct gggcctgcat gatgcgttag tcctcattga cgacgccaag catttgtctg
     3001 ccgatgaaga caaaaacacc atgcagacgt actcacgtat gaaaaaccag tttaccgtca
     3061 gcattaacca gccattgaat atcgcctatg aagattacgg ttcgctgttt atttccggta
     3121 gctggacgta ttactgggcg gcgaacaata gccgcactga atataatgtt ggttacagta
     3181 aaagcgtttc gtggggcagt ttcagcgtca acctacaacg tagctggaat gaagacggcg
     3241 agaaagatga cgcgatgtac gtcagcgtta gcgtacctat tgagaatatt ttaggtggca
     3301 aacgtaagtc ttctggtttc cgcaatttaa atactcagct caataccgat ttcgatggtt
     3361 cacatcagtt gaatgttaac agttccggta acactgaaaa caatctggtg aactacagtg
     3421 tcaacgcagg ttatagcctc gataaaaacg ccggcgattt agcctctgtt ggtggttatc
     3481 tcaactatga atctgggtta ggcggtattt ccgcttcggc ctcggccact tctgataaca
     3541 gccaacagta ctccatctca accgatggcg gctttgtatt acacagtggt ggtttaacgt
     3601 tcactaacaa cagtttcagc agtaacgaca cgctggtgtt aatcaacgcc ctaggtgcta
     3661 aaggcgcacg aatcaataac agtaataacg aaatcgatcg ctggggatat gccgtgacgt
     3721 cctctgtcag cccatatcgt gaaaaccggg taggtctgaa cattgaaaca ctggaaaacg
     3781 atgttgaact gaaaagtacc agcgccacca ccgtaccacg tagcggctcc gttgttttga
     3841 cccgtttcga aactgacgag gggcgttctg ccgtgctgaa tattactgcc gccaatggca
     3901 aatccattcc gtttgctgcg gaggtttacc agggtgaggt gatgatcggc agcatgggcc
     3961 agggtggtca ggcatttgta cgcggtatta acgacagcgg ggaattaatc gtgcgctggt
     4021 atgaaaacaa ccaaaccatt gactgtaagt tgcactacca gttcccggcg cagccacaaa
     4081 cgcagggaag caccaacacc ttattactta acaatcttac ctgtcaggta gcaaatcact
     4141 aatatggaaa attctatgaa aaggatacta ctaacatccg cgttaatagg cctgggttta
     4201 ccggcggttg gctctgcaac agatcttaat gtcgatttta cggccaccgt attagccacc
     4261 acctgcacga taactatagt ggaagatggt ggtccagcag tgaccggtag taacgatgaa
     4321 tacagcctga caattcctga tgttggtctg gataaagtcg ctaccgctgc gcccgaagct
     4381 caggccaact ttaaattaaa ggcctccgat tgcagcaatg gttactcaaa gatttttacc
     4441 acacttacag gaacaacggt ttccggtaag ttaattgtta atgaagcaac ctctggcgct
     4501 gctggggtgg ggatggggat taagcgtaga gataccgcag actcgacttt cttcacaccc
     4561 aataatactg acaaatttga atggagcgcc gatgagaaag ccagcggtgt gcctctgacc
     4621 gtagcactgc gcgaaacaac ggcgggagca ggacgcaccg gtgcgtttca ggcaaaagcc
     4681 acctttaact tcacctacga ataacaggga cagccaccat gaaattaaaa gtcatcgcta
     4741 cactgattgc tactgttgcc gtgggtgtaa gctttaacag caattttgct tctgcgagta
     4801 caacgtccgc ttctttaacc gtaaacagta acctgactat gggtacctgc agtgctcaga
     4861 taatggataa tagtaataaa gtgatcaatg aagtggtctt tggcaatgtt tatatttctg
     4921 aactcggtgc aaaaagcaaa gtgcaacagt ttaaaattcg ctttagcaat tgctctggcc
     4981 ttccccaaaa cagcgcccaa atagtgctgg cacctaatgg tatatcctgt gctggttctc
     5041 aatcgtcatc ggcgggtttt tctaacaagt ttactgacgc tagcgcagca accagaacgg
     5101 ctgtggaagt atggactaca gatacaccgg aaagcaatgg cagtacgcaa ttccattgtg
     5161 ctcaaaagat accagtgcct gtgacgcttc ccgccgacac cacaactcag ccttacgatt
     5221 acccgttaag tgcacggatg accgttgcgg aaggtagatt ggtaaccgat gtaagaccgg
     5281 gtaatttccg ctctcccacg actttcacga tcacttatca gtaatagcgg ataaggatat
     5341 tagaatgcta tgcaggcatc ataaaatcgt acacttttta gggctggcaa cagcccttat
     5401 cacgcctttt gcctattccg gccaggatgt ggatctcacc gcaaagatcg tgcccagcac
     5461 ctgtcaggtt gaagtcagta ataatggcgt tgtcgatctc ggcacagtga cgctggacta
     5521 ttttgccgac aatgtaaccc cgacaacgga ttatgcgggt ggcaaaacgt ttaatgtgaa
     5581 tgtggtctct tgcgataaca ttcaaacgac acagtcgcaa atgaaattag attttcagcc
     5641 gcaggcggga tcgcttgcac aggcgaataa tcaaattttt agtaatgaat atgaacagca
     5701 ggctaccggg gcgaaaaacg tcggcattgt gattttttcg gcccaaccga atcagcagac
     5761 atttaacgtg cgaggaacgg atggttcctc aacagcaatt tattcggtag cacctggcaa
     5821 tgctgttccc tcaacctgga ccttttattc ccgtatgcag cgggtgaata atgcccttcc
     5881 tcctgaatcg ggaatggtga gaagccaggt tattgttaac gtatcttacg aataataagg
     5941 ataattatgc aaaggaaagg caataaactg ttgattcagt tatgcagtgt gatactgcta
     6001 ttttttacca catcctggta tgcattggcg aatgaatgtt atatagagag aaatgctgaa
     6061 ggggattatc acatgaagat aagctctact cagcttagtc tggcgtcaca aatggtcgag
     6121 gttccgacag aaatagccga agctacatgg gatgtaaata ttcaactaag aggcgatgcc
     6181 atagggtgta aatctcttgg ggatagtaag gcagttcact ttcttaatac agctgaccca
     6241 agtttaatat ccacgtacac cacaacgaat ggcgcagcgt tattaaaaac aactgttcca
     6301 ggcattgtgt attctgtcga gttattatgc cttagttgtg gtgccgcaga tgaacttgat
     6361 ttatggctac ctgcacaaag tggcgcagat aacttcatac caagcaccca gacgaaatgg
     6421 gcctatgagt acagtgatca aagttggtat ttacgttttc gcttattcat aactcctgaa
     6481 tttaaaccca agaatggtgt ttccagcgga acaacgatag caggaaagat tgcgtcatgg
     6541 tatataggta ccaatgacca gccgtggatc aacttttaca ttgacaatga ctctttaaag
     6601 tttttcgtcg atgaaccgac ctgtgcaaca gttgccctgg cacaagatca gggcaacgtc
     6661 agtggcaatc aggtaacgct tgggaacagc tatgtttcgg aagtgaaaaa tgggcttacg
     6721 cgggaaatcc ctttttctat ccgtgctgaa tactgttatg ccagtaaaat tacggttaag
     6781 ttgaaagcgg caaataaacc cagcgatgcc acactggtgg gtaaaacgac tggctcggct
     6841 tcaggcgtgg ctgtaaaagt aaattcaact tatgacaata gcaaagtatt gttaaaagca
     6901 gatggtagca acacggttga ctacaacttc gccgcctggt caaacaacct gctgttttta
     6961 ccttttacgg cgcagctggt accggatggt agcggtaatg ctgtcggtgt tggaacattt
     7021 tcaggtaacg cgaccttctc ctttacctac gaataagtaa aacgtcattc cctcctcatc
     7081 gcaccagagt tctgctctgg tgcgaacatt tccccacact aataaccatt gcctccctgt
     7141 tttcggctgt tagaatgcga tcaatctgac tttagccatc aggatacgtt atgaaaccga
     7201 ccaccattgc ttcattgcag aaatgtaagc aggaaaaaaa acgcttcgcc accatcaccg
     7261 cctacgacta cagcttcgca aaactgtttg ccgaggaagg gctgaacgtg atgctggtgg
     7321 gcgactcgct ggggatgacc gttcaggggc atgaatctac ccttcccgtt accgttgaag
     7381 atatcgccta ccacactacc gcagtacgtc gtggcgcgcc aaactgcctg ctgttggctg
     7441 acctgccgtt tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta
     7501 tgcgtgccgg tgctaacatg gtcaaaattg aaggcggtga gtggctggtc gaaaccgtaa
     7561 aaatgctgac cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag
     7621 tgaatatttt cggtggctac aaagttcagg ggcgcggcga tgaagcgagc gatcgactgc
     7681 ttagcgatgc attagcctta gaagccgctg gtgcacagtt gctggtgctg gaatgcgtgc
     7741 cggttgaact ggcaaaacgt attaccgaag cgctggcgat cccggttatc ggtattggcg
     7801 caggcaacgt taccgatggg cagatcctcg tgatgcacga tgcgttcggc attaccggcg
     7861 gtcatatccc taaatttgcc aaaaatttcc tcgctgaaac gggcgacatc cgcgctgcgg
     7921 tgcggcagta tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc
     7981 attaaggagt gacgttgtgt taattatcga aaccctgcca ctgctgcgcc agcaaattcg
     8041 ccgcctgcgt atggaaggca agcgcgtggc gctggtacca accatgggca acctgcatga
     8101 tggccacatg aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat
     8161 tttcgttaac ccgatgcagt tcgaccgccc ggaggacctg gctcggtacc cacgcacctt
     8221 gcaggaagac tgcgaaaagc tgaacaagcg caaagtggat ctggttttcg cgccctcggt
     8281 aaaagagatc tacccgaacg gtactgaaac ccatacttac gttgacgttc cagggctttc
     8341 gaccatgctg gaaggcgcca gccggccggg gcattttcgc ggcgtgtcga ctatcgtcag
     8401 caagctgttt aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca
     8461 actggcgctg atccgcaaaa tggttgctga tatgggcttt gatattgaga ttgtcggtgt
     8521 gccaattatg cgcgccaaag acggtctggc actgagttcc cgtaacggtt atctgacggc
     8581 agaacaacgc aaaattgcgc ccggtctgta caaagtttta agttcgattg ccgacaaatt
     8641 gcaggctggc gaacgggatc tcgatgaaat tattgctatt gccgggcaag aactgaatga
     8701 aaaaggcttc cgcgccgatg atattcagat tcgcgatgcc gacacattgc tggaagtctc
     8761 tgaaaccagc aaacgggcag taattctggt agccgcctgg cttggcgatg ctcgcctgat
     8821 cgacaacaaa atggtcgagc tggcgtaata cttatactct aaataattca agttgcaagt
     8881 tgcaggaagg cggcaagcga gtgacaaatt cgtcaggaac gaatttgccc agccgcaggc
     8941 tggccttcgg tgagagacat ggatgtctct cattaatccc caggagctta cataagtaag
     9001 tgactggggt tagcgaacgc agacgcagca catgcaattt gaagtatgac gagtataact
     9061 ggcgctacgg ctgatggcgc caattattaa tttgccccac gtcatggcga tggcgtctgt
     9121 aagcaattat tcataacgca gacaacttag ttaatcagcc tatcaatctc agcaagagat
     9181 aaatttgtca tctctgcaac gtcttcccga gacatccctt tactcagaaa acgctgggcg
     9241 aattcttgcc tctcctcctg tcttccctgc tgaatcccct tctcaatccc tttctcttca
     9301 aaccactgtg ccagcgtcat catagacttc cctcccgttt ccctgtctct cagcacacca
     9361 taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg catggcaact
     9421 aactgacttt cgctagtgta cccttcgtca atcagcgtga ccagttgctc cagcaataac
     9481 attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat ccgccgatgt
     9541 tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg actgttatag
     9601 acgcgtcgcg ccagctccgg cgagtaaaac atatcaaacc agcacattga gagcggataa
     9661 ggtgtggcct cgccctgata aaagagaatc ggcactacca gcggtagttt gtcgtgatcc
     9721 gcctccagat gccgatgcat ggcggctata gaataacgca tcatgcgaaa ggccattttc
     9781 ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt gccctgcatt
     9841 tgcacggaat agagcacgtc cgtgctgtgt cctttaaggc tctcttcaat gaaactcccc
     9901 gactctaaat gaagcgtgtt gaggtcacaa agttcgcgta attccactgg caaatgtatc
     9961 tccagaaagt cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac cgcgtcatgc
    10021 ggtgtggtac tcggtgcatc catcttccct gtcttccgtg ttgtcttgcg tgaaggcagt
    10081 atccagtcaa ggcgtgctat cgtcagccac ctctttattg tttaccgagc agcgttcaga
    10141 gaaatatcaa ttcgttacag gcgatacatg gcacgcttcg gcgcggtacg caaactgctg
    10201 atgcaaatgc cgtcagatga gtagacacta aacaaaaatc gggcaatact gcgtgagaat
    10261 ttcctcaaag caggcaccgc ctgcttcgtt aacgacaggg tagaaaggta gaagttatga
    10321 ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg gacctgcact
    10381 atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg
    10441 aagccattga tatctggaat gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg
    10501 cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc gccagtgtcg
    10561 gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct cgcacctggc
    10621 gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc
    10681 cggtacaggt tgcttgattg ttatccctgc ggctggttac tcaccagccg cgacatcgtc
    10741 tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt atcccccggt
    10801 tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc
    10861 acggctttgt cattaaagcc gccaaacgga tacgaaagat accagacatg cggattaaat
    10921 tgcgccagag cgcgacgtga acgtgcaaaa tcaaacagaa tattgtgctc actacggctc
    10981 agtaatatgg gtcggcgata accatctacc cgatgcaaaa aatgggtatg tgactggaaa
    11041 tcaaatacat cgcgaatttc gttaagctca gaaacgctca taaattgcag cgattttggt
    11101 tcccacttct gcgggtgacg tttgatgcgc gaggtaacaa taaacgccgt cgccttcatg
    11161 ccatattgtt tcaacacagg atacgcatag cggctcactg acttgaggcc atcatcaaag
    11221 gtaatcacca ccgctcgcgc aggaagattg atcttattct tcacgtaacc ttccagctgc
    11281 accatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatctggtt attgaaagcg
    11341 cgtaccgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc gcgcagaata
    11401 tggtgatagg ttagcaccgg aaggccatta tcgggttggg catccagcgc gctgatatag
    11461 gccagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc tttcagttta
    11521 tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact gcccacactc
    11581 ggcgcgttat agaccggcgt atctttccag gtaactaagt tctgattact cagcggcttg
    11641 ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc aagatgacct
    11701 ttatcgataa accctttatc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc
    11761 tccacggcaa tgatttgtcc ggcccgaata ttccccacgg tcaccatctt gtcaccaatt
    11821 tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcactg
    11881 acacttgcgg taaacagcat cagcaggaga ataacagctt gtttgtacat gaaaaaatca
    11941 ccgagggtga aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc gggttaatac
    12001 tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgctggtg ccaaatacta
    12061 ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa actctgaact ggtcatggtt
    12121 tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat cacttcgcaa
    12181 cgggagtgtt tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc ggctatatcg
    12241 gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca catcatgcgg
    12301 ctgagcatat tagaacttaa tccgcgccag aaattcacgg cccggcactg aagaagcgcc
    12361 ccatgttttt tctccagcac atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt
    12421 acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga ccattgttga
    12481 taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg aaatagcctt
    12541 agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa aacgccacaa
    12601 taaagaccac cagtacgcca aaggtagtga ccagcggaac atcattgata ccgaggaagc
    12661 cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc ccttgccaga
    12721 acggcggcaa caaagtcagt gagtaaaaga ccccacccaa atacgtgagt ggcgttaaca
    12781 caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacaccg ttcagcaaac
    12841 ccgcaaggga gaacaacacc gccgtgagca ccagggttaa ggcaacgaat acccacgaat
    12901 gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca acaaacagac
    12961 cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga accggcgcta
    13021 ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac gcgacgttgg
    13081 cgtaggcgtt ggtgatcacc gacatcatga tcagccccgg tacgatgaac tgcatgtagc
    13141 tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaaaata ataaagtaaa
    13201 gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata aagcgatgga
    13261 tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg cgcgatctcc
    13321 ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt tgttacgcat
    13381 acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga tcccctgctc
    13441 acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat agccatcgag
    13501 cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt tcagcttcgc
    13561 cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa tgccgatatt
    13621 gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg tggtgccttt
    13681 gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat ccacgcctgc
    13741 agtcggctcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg caatcattaa
    13801 acggcgcttc atcccgccag ataacatgcg cgcacgttcg ttgcgttttc cccataaatc
    13861 gagctgctta agatattttt cgctgcggat gtacgcctct ttgcgctcca caccgtagta
    13921 ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt taaattcctg
    13981 cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat cgtaaccaaa
    14041 tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga tagtggtcga
    14101 tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc
    14161 tattccacga agcgcctgaa cgccgcctgg atagtttttt ttaagctgtt gaagttccag
    14221 tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt ggtttaaatc
    14281 gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc
    14341 atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa
    14401 gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt
    14461 ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc
    14521 tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg cctttccgtg
    14581 gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg ccactacggt
    14641 tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa caactggctg
    14701 ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcgacgaaat gccgcaagag
    14761 cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa cctgggccac
    14821 tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggct
    14881 tatggcattc acgacggctt gctgcgtgat ctggatgtta ctgccaccaa ccgcgaaacc
    14941 cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc caaccacaaa
    15001 taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg tccagcagaa
    15061 tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca
    15121 ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc acttcacgac
    15181 gggacggttt atccagcagc gtacaaatcg ccaacgactt cggttcgcgc aggcttaaga
    15241 tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca atcagcacat
    15301 ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg gtggtggaca
    15361 tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc tgaacttcac
    15421 ggcacaggtc cgccataaac ataaatgagc cacgcagcag acccaccagc accatatcgc
    15481 tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata cgcgctttaa
    15541 tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc atatgattta
    15601 aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa gacacgcagt
    15661 ataccagcaa aacgattcat tcgcggaatc tgttaataaa gctcaaattg tgatgacgat
    15721 cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg aaatggtgtc
    15781 tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta acagcccttt
    15841 ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg
    15901 gctcctggta ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg atgctgtggc
    15961 gcagtaaacg tgccgcgctt tggctatacg ccgccctgct gctcggcacc atgatttggg
    16021 gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgat attctggtct
    16081 tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg
    16141 gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tagtatcctg acctgggccg
    16201 gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca cctgcggaag
    16261 ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag gaaggtcaac
    16321 gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa gaagcctggg
    16381 tgttccgtac tggcgatgtg aagcagccga acgatccggg cgaaatcacc aatgaagtga
    16441 cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc
    16501 tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa actaacgagt
    16561 ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt
    16621 cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat gatggtcgcc
    16681 tgattgcgat taacgctgaa aacggcaagc tgtgcgaaac cttcgccaat aaaggcgtgc
    16741 tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg acttcgccac
    16801 cgattatcac cgataaaacc atcgtgatgg caggttcagt taccgataac ttctcaaccc
    16861 gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt
    16921 ttgatccggg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc tttaccttta
    16981 actcgccaaa ctcgtgggca ccagcggcct atgacgcgaa gctggatctg gtgtatctgc
    17041 cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt
    17101 atgccagctc gattctggcg ctgaatgcca caaccgggaa attggcgtgg agctaccaga
    17161 ccgttcacca cgacctgtgg gatatggatc ttccggcaca gccgacgctg gcggacatca
    17221 ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc aacatttttg
    17281 tgctcgatcg tcgtaatggc gaactggtgg ttccggcacc ggaaaaaccg gttccccaag
    17341 gtgctgccaa aggcgattac gttaccccta ctcaaccatt ctcggaactg agcttccgtc
    17401 cgacgaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt
    17461 gccgcgtgat gttccaccag atgcgctatg aaggcatttt cacgccgcca tctgaacagg
    17521 gcacgctggt cttcccgggt aacctgggaa tgtttgaatg gggcgggatt tccgttgatc
    17581 caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg aaactgatcc
    17641 cacgcggtcc gggtaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat
    17701 ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac
    17761 catttggtct gccatgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa
    17821 ctaatgaagt ggtgtggaag aaacgtattg gtacgccgca ggacagtatg ccgttcccga
    17881 tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg
    17941 cgggtaacgt gctgtttatc gccgctacgg cagataacta cctgcgcgct tacaacatga
    18001 gcaacggtga aaaactgtgg cagggtcgtt taccagcggg tggtcaggcg acgccaatga
    18061 cctatgaagt gaatggtaag cagtatgtgg tgatctccgc aggcggtcac ggttcatttg
    18121 gtacgaagat gggtgactat attgtggctt atgcgctgcc ggatgatgtg aagtaagact
    18181 tgctcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
    18241 cagcctacaa aattgtgcaa aatcaatgga ttgcacaggt aacgtaggcc tgataagcgt
    18301 agcgcatcag gcagatttgc gtttgtcatc agtttaatgc ccggagagat ccgggcatat
    18361 tcccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc ttcatgctcc
    18421 agcagatggc agtgcgccat ataagcacgt tctttcggtg cgtcgtgatt aaacttcacc
    18481 agcacttcac tgacattgcc ttcgacctta acggtatctt tccagcccgc gcgatgagcc
    18541 gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat atggaacgga
    18601 tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc tttcgccgcc
    18661 gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc atggtggaaa
    18721 tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg gcccatcatc
    18781 tggctgtgat ccattccgac catcgcctga tcgccatatt tctccattag catctgcatc
    18841 cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt cagcccttcc
    18901 agcgaaggta acgcaggcag gctacttaat gtgtctggca aagcaccgga agcactaata
    18961 gcaatcggct gaatccgcat taccggatga ggcttatcaa acggcgcaat cgccatcccc
    19021 atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac ctccaccagc
    19081 acttcaaaac gctcgcccat cagcaccggc agctcgttca ccttcaccgg ttcaggtagc
    19141 agaccaccgt cgctggcaat cacataaagc gggcgattgt cgctggtggc gaaattgagc
    19201 gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg ggcagcgtgt
    19261 tgcgggtaga ttgcaccgtt ggtcagcaac gtatcgccaa accagcccac ggcggcggtc
    19321 atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc ctgaacgatc
    19381 accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat ctcgtcatct
    19441 tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt gccatgttga
    19501 tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac cgagcgcttg
    19561 ccacctggcg gaataattcc ctgtgggccg ccgtcgactt cacccggtac ttccagcccg
    19621 tgccagtgca acgtcgtctc ttccgtcagt tggttataga tatcaaccgt taccgctttg
    19681 ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca ggtagttgca
    19741 gttttctcgc caaaggtaga ctgacctgcg ccaatagtta actgaatgcg attacgggca
    19801 tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa gactgcacgg
    19861 ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa gaaatcacga
    19921 cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg ttgagcatag
    19981 tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat aatggtcgtc
    20041 gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa gcaatgaaga
    20101 cgtttttcag aacagtgtta ttcggcagcc tgatggccgt ttgcgcaaac agttacgcgc
    20161 tcagtgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc tttctgaaga
    20221 acgattgtgg ttaccagaac ttacctaacg ggcaaattcg tcgcgcactg gtctttttcg
    20281 cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa gctctcggtg
    20341 aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaag tgcaaggccc
    20401 tggctcgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata cctttttgta
    20461 gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc tttttttacg
    20521 ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat gaaacgctac
    20581 acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa aagaccgatc
    20641 accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg ctggatggcg
    20701 tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc catgttccgc
    20761 tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac ggtgccatgc
    20821 tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa atcgatgcgg
    20881 gtgtcgtgtc gttctgccgt cagtatctac ccaaccataa cgccggtagc tacgacgatc
    20941 cgcgctttaa gctggtgatc gacgatggcg tcaatttcgt taatcaaacc agccagacct
    21001 ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc cttttcactt
    21061 cggcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc gtcgcacaaa
    21121 acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa ctcagccatt
    21181 acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc ggtatcatga
    21241 cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa attattcagg
    21301 cgcgttttct cgcctctggc ctgaaatgcc gttattacaa tccggcagtc catacggcag
    21361 cttttgcctt accccagtat ctgcaagacg cactggcttc acagccgtcc taaggagaag
    21421 ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa agtctgagtt
    21481 tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt tatattgctt
    21541 atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa acctgttcca
    21601 ttatcggggc taatattctt aacatcgccc gccaggatta cgaaccacag ggtgccagcg
    21661 tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa acagaacacc
    21721 ccggcccact gccagaaacg gtcgttgccc atctcgataa aagtcatatt tgcgtacata
    21781 cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat attgaagtct
    21841 ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac cagcttgagt
    21901 ccgatatcgt aaccattgat tatcgcgtgc gcggttttac ccgcgacatt aacggtatga
    21961 agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac gatatgaagg
    22021 cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat accaagatgt
    22081 tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa gatttaaccg
    22141 acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag atttattacg
    22201 ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg ccagatttat
    22261 ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat cctcaacgcc
    22321 gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt catcgccagc
    22381 gaattccagt tgattggcgc gaaccatcac ctcttcgccg tccatccata gcgtatactc
    22441 atgccctgcc cgctgccagg aacgttcgct acctttcagt gcgtgcgctg cttgttccac
    22501 ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc cgaccacttc
    22561 atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa attcgtaatc
    22621 catagtgtgt tcctctgctc ccggcaatgc accgaaatcg cattgcgcca ttgatgtaat
    22681 tatcgcagcc ttaccggaga aaaacagcgg aacggcgatg gtttagaaat aaaaaaatgc
    22741 ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg ctgacttttt
    22801 aaaccgcagt ctggaaaatc accccatcgg ctttctcggt gtactgagaa agctggttga
    22861 agttcagata acggtaagta tcaacggctg ttttatctac ctgcgccacg taggtctggt
    22921 actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agcagccagt tccgcagaag
    22981 ccaggaagac attcgcgcca gtacccagac ggttcgggaa gttacgggta gaggtggaaa
    23041 ccaccgttgc accgtccgcc acacgcgcct ggttacccat acacagggaa cagccaggga
    23101 tctcgatacg cgcaccactc ttaccgaaga cgctgtagta gccttcttcg gtcaactgtg
    23161 cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga cctttgtgcg
    23221 catccagcag tttacctgcg gcacggaagt gaccgatgtt agtcatgcaa gaaccgataa
    23281 acacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtcg tccgggtcgt
    23341 tcggagcaca gaggattggc tctttaatat ccgccagatc gatgtcgatc actgccgcgt
    23401 attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt tccatgccct
    23461 gaatacgacg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc cacttcagca
    23521 ggacgatgtt ggagttcagg tattcgatga ttggttcttt gttcagcttg atggtacaac
    23581 cggcagcaga acgctcggcg gacgcatcgg tcagctcaaa ggcctgctca actttcagat
    23641 ccggcagacc ttcaatttct aggatgcggc cagagaagat gtttttcttg cctttcttct
    23701 caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc agatcgcgca
    23761 gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc ggcatatcca
    23821 gcggcattac gcctgttgcg gctgcaaatg ccaccagacc agaacccgcc gggaaagaga
    23881 taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc ggcagcagca
    23941 tacggttcag ccaggagtgg attacgccgt cacccggacg cagcgacaca ccgccacggt
    24001 tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc ggatacgccg
    24061 cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac gccaggtctt
    24121 tcagttcatc acgggtcatc gggcctgtgg tgtcctgaga gcctacagaa gtcattttcg
    24181 gttcacagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct accattttct
    24241 gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg aacacatcac
    24301 tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgaccg ataatcagcg
    24361 gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt tcgaaggtcg
    24421 ccagcagttc gccggtttcg tggttacgta cttcaccttt gtacgggtaa acgtcaatta
    24481 cgtcgcccat gttcaggtta gagacatcca cttcgattgg cagcgcaccc gcatcttcca
    24541 tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg ccgcgtttgt
    24601 tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg gcggatttac
    24661 gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct ttctgttgca
    24721 gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca ataccttcac
    24781 gggcgttttt cagcatcgcc agcgcgtgca atgggatatc cgggcgtgac cacgcatccg
    24841 gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttgaagacg gtaacggtca
    24901 gtttttcagc cagcgccggg cgattcagga accattcggc atcagcccag gactgcataa
    24961 cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag aagttatcga
    25021 acatcagtag tgtgtgagaa agtgctttgg cagcgatcgg tgccagtttg gcatcatcca
    25081 gcgcatcgat cagcggatga atgttgtaac caccctgcat agtacccagc agttcgatgg
    25141 ctttttccgg agtcagcagt ggggatttgg cttcgccttt ggcgacagca gccaggaagc
    25201 ctgctttaac ataggcggct tcatcgacgc caggaggaac acggttggtt aacagatcta
    25261 acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc gcggccattt
    25321 ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct acgtgcttac
    25381 ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg caggcgattc
    25441 tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg caacgggcag
    25501 aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata aaaaagtggc
    25561 tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt taattcgcat
    25621 ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac taccgcctct
    25681 ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg atttccttga
    25741 gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc taatttgcag
    25801 tgccgggctt tgtgccgcct ctgccgggct atatataaaa agccgcacag tggaagcgcc
    25861 tgtggaaacg caatcgacac aactggctgt gtctgacgct gccgcagtta cgcttcctgc
    25921 aacggtttcc gcacctcccg taacacccgc cgtcgtcaaa tccgcattca gcactgcaca
    25981 aatagatcaa tgggtcgcgc ccgtcgcgct gtatcccgac gccctacttt cgcaggtgct
    26041 gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc acgataatcc
    26101 acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg acgccagcgt
    26161 taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc cgcaatgggt
    26221 gcaaaacctg ggcgatgctt ttctggccca gccgcaggac gtgatggact cggtacaacg
    26281 attgcggcaa ctggcacaac aaaccggctc gctgaagtca tcaaccgaac agaaagttat
    26341 taccacaacg aagaaaactg taccggtaac acagacagtc acggctcccg tcataccatc
    26401 caataccgtt tcaactgcca accctgtcat tacagagcct gcaacaaccg tcatttccat
    26461 tgagcccggc aatcctgatg tggtctatat tcccaactac aacccaaccg tggtttacgg
    26521 gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg gagaaccgtt
    26581 tgttgacagc tttgtacgcg gattcggcta tagcatgggc gttgctacca cgtacgcact
    26641 attcagcagc atcgactggg atgacgacga tcatgaccat catcatcatg acgatgataa
    26701 ttatcatcac cacgatggcg gtcatcgtga cggtaatggc tggcaacata acggcgacaa
    26761 catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta ctgataagaa
    26821 tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg atcaggatat
    26881 ggcaaagcgg tttcatcaaa ctgatgtcaa cggcggaatg agtgccacgc agctacctgc
    26941 tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac gaacacacgc
    27001 cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt ttaatgaagc
    27061 tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac cactgaccca
    27121 gcaacaaaag gacgccgctc gtcagcgtta tcagtcagct tctcctgagc agcgccaggc
    27181 agttcacgag aaaatgcaga ctaacccgca gaaccagcag cgaagagagg cagcgcgtga
    27241 gcgcattcag cccgcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa tgcagactaa
    27301 cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt attcagtcag cctcgcctga
    27361 gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac agcaacgtga
    27421 taacgcccgc cagcgtgttc aatcagcatc acctgaacaa cgtcaggttt ttcgggagaa
    27481 agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgcca ggctgaataa
    27541 cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc gactggaaag
    27601 gtaaattaca ggcgtaaaaa aagcggcgtg gttagccgct tttttaattg ccggatgttc
    27661 cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcagt aatgctacct
    27721 tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc gtggatggtc
    27781 agtgcgatgt cttcagcatc acaacccatt tcaattgcca ggccgatttc acccagcagc
    27841 tcgccgccgt tagtaccgac aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa
    27901 atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga agcagcccac
    27961 gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc agtcagacct
    28021 acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtag
    28081 tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc
    28141 agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt ggtacgcagc
    28201 tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt gcctgcgtcg
    28261 aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt
    28321 tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt cgcttcaacg
    28381 gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt ggtgaagact
    28441 ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac cacgtcaatc
    28501 tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc gccacccatt
    28561 accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtagagtc ccagatacgc
    28621 ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc aatgatcgcg
    28681 ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt gttagccccg
    28741 gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga
    28801 ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc gatatcggtt
    28861 ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt
    28921 gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg
    28981 gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg
    29041 gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc
    29101 atgacctcta tatatttatc tccggcgggt ctgacgtttt ttttatgacg cccattatcc
    29161 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    29221 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    29281 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    29341 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    29401 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    29461 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    29521 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    29581 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagttcg
    29641 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    29701 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    29761 gaactgttga agcgaggcat ctgctcaagc gctgcagcaa cggctttcat gatgaagaca
    29821 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    29881 aacgcttcca gctcggtgat atcggttttg tcgaagtgag taacatgcgg gatcatcacc
    29941 cagttacggc tcaggttcgc accagaaatt ttctggatac ggcccagttc cacttcttcg
    30001 atttcaccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    30061 ccagtcgctg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    30121 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgcaag gttaacgcca
    30181 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc attttcagca
    30241 aattcagatt tgccttccgc tttcgcagcc ggtgctgctg ccggggcttc agcttttgct
    30301 gccggagccg gcgctgctgc ttcctgtttc gcaggagctg ccgcaggcgc tgcgccttca
    30361 acttcgaaga tcataatcag cgagccagtt ttcactttat cgccaacgtt gactttcagt
    30421 tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg
    30481 gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact
    30541 tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc cgctggtgca
    30601 ggggccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc
    30661 gccacttcga agaccataat cagcgagccg gtagacactt tgtcgccggt gttcactttg
    30721 atttctttca cggtgcctgc aaacggagcc ggaacttcca tagaagcttt atcgccttct
    30781 acggtgatca gcgactgctc agcttcaact ttgtcgccaa ctttcaccag gatttcggtc
    30841 acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc ggcagcctcg
    30901 gggactgctg ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct
    30961 gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgagtttt atcgccaaca
    31021 gagactttga tctctttaac gatacccgcc tgcggagacg gaacttccat agaagctttg
    31081 tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg
    31141 atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt
    31201 attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg
    31261 cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg
    31321 ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt
    31381 cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt acgtaagtac
    31441 ggacctgctc agcgaacagt ttcatatagt cggtagatgc cacagccgga gcgtcgttca
    31501 tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg ttccagcgtt
    31561 cacaatcctg accatcacgc gccagttcgg tgaaggaggt cacgctataa acgtcagaac
    31621 ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatagaac
    31681 cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac
    31741 ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca
    31801 gcgtggtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt
    31861 catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggaa
    31921 tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg
    31981 tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg ccagccgccc
    32041 agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag aacggaatca
    32101 tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacaa cctgcgccca
    32161 gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct
    32221 gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga
    32281 acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt
    32341 tgatcgactt gttcttcagc atcacgttca gggcacgaac gaaagcgata gtggtagaga
    32401 tctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggcttggc agctcaagct
    32461 tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga cgctgtgcgt
    32521 gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat
    32581 ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacaccg tccatgttca
    32641 ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt
    32701 taatggtatg agcaaggatt accgtcgctt tgcctttggt ttcctgcgct ttcttgaatg
    32761 cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag
    32821 tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt
    32881 acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct
    32941 ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca
    33001 ctttgatcac gttccagcca gcaccttcga agataccttc cagttcgttg atgatcttgc
    33061 cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg aagaccaggt
    33121 tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt
    33181 caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt
    33241 ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga cccatagata
    33301 cggtcgggaa ctgccagaat tccggcatca atttcgggtg cggataggaa gagaggccat
    33361 tgccgtgaac ttcctgacgg aagttatcca gctgctcttg agtcagacga ccttccagga
    33421 aagcacgagc gtacacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat
    33481 cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag
    33541 actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagaaccg
    33601 tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg
    33661 gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata ccggtgcctg
    33721 cggctacgtt gacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag
    33781 cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt
    33841 cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat
    33901 aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc aataagacag
    33961 gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct tctggataat
    34021 ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg
    34081 cggctttctt cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc
    34141 gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg
    34201 tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg
    34261 acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg
    34321 gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg
    34381 ccagactgct gcgccagttc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca
    34441 tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg
    34501 agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag
    34561 ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc
    34621 ttcgcttcga gacgttgaat cgcctcacgc aatgagggac gggagacgtc aaactgtttt
    34681 gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa
    34741 aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc
    34801 atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt
    34861 gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa
    34921 actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa
    34981 tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa aatccgaatt
    35041 gaaccgattc acttaccaat tttgtgattt ttaattcaat taaaacgaat ttaaattcat
    35101 tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca
    35161 tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac
    35221 ggaagtcttt ttgtaactat tcaaacttct ttgatgtaaa caaattaata caacaaacgg
    35281 aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt
    35341 tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata
    35401 ttcagcttat cgcgctgggt ggcgcgatag ggacagggtt attcctgggt agcgcctccg
    35461 taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct
    35521 ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta
    35581 gccactttgc ttataaatac tggggcagct ttgctggctt cgcttctggc tggaactact
    35641 gggtactgta cgttttagtt gccatggcag agctgactgc tgtgggtaaa tacattcagt
    35701 tctggtatcc ggaaatccca acctgggttt ctgccgccgt gttctttgtg gtgattaacg
    35761 ccatcaacct gaccaacgta acagtgtttg gtgagatgga gttctggttt gccattatca
    35821 aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggtaacg
    35881 gcggtccgca ggcaagcgtt agcaacctgt gggatcaggg cggtttcctg ccgcacggct
    35941 tcaccgggct ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg
    36001 tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc
    36061 aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga
    36121 tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag
    36181 gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt
    36241 acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa cagggtaacg
    36301 cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt
    36361 ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg
    36421 gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg
    36481 cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc
    36541 tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggtggta ctggtgatta
    36601 tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctggtggtgt
    36661 taggtatcgg ctatctgttt aaagagaaaa ccgcaaaagc cgtaaaagca cattaatctc
    36721 tctacgccct cacccgtaca gggtgagggc aataatcttt acaccaacgc cccgtaaatc
    36781 gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct
    36841 gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga
    36901 cgcgttaata cctgatattg cgaagtacgg aaatcaccca gcgaagcaaa ccaggccggt
    36961 aacgctttct caccatgacc gatcaaggca tataccacac ctgcaagacg aaccggcacc
    37021 caatccagta catgaagcac tgcatcaatg ccggactgta aacgatgatg cggcgtctgg
    37081 tatcgcgcca gccagtattg ccatgcacgc aaaaacgcat accccatcag cgtaacgggt
    37141 ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc
    37201 cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg
    37261 ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa
    37321 gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa
    37381 ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt
    37441 aacagtaaaa aagtcacgcc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa
    37501 aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc
    37561 aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct
    37621 ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc
    37681 gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatccgga tagcaatcaa
    37741 tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagcgtat
    37801 cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcgcgc ccctgatact
    37861 gagagactcc cgcatgccat gcacgtttat cgaaaggaac atactggact atttcaccat
    37921 cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat
    37981 gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc
    38041 ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccgggcggc
    38101 aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca
    38161 tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta
    38221 tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctataacc ctgacacccg
    38281 acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct
    38341 gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc
    38401 ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgcggcaa
    38461 acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca
    38521 tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc
    38581 ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt
    38641 tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga
    38701 tacgcgtaaa accttacccg gtctgcgttc agcgctgaaa tacgcggtac tttgcggcgg
    38761 cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat
    38821 tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcacc cggatgcgcc
    38881 agtagaagtc gaagtagaga atctggaaga acttgatgaa gccctgaaag caggagccga
    38941 tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa
    39001 cggcaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc
    39061 cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aagcacgtac aggcactcga
    39121 cctttcaatg cgttttcgct agttgctgac agatttatcc gctccatagt gggcggataa
    39181 tctccccttc cccaaccttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg
    39241 aaataaaaaa cctcggaagc agctttcccg tttcgttttt tgcccctcgc ctgtgctgat
    39301 ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg
    39361 gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg
    39421 gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct
    39481 ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct
    39541 gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga
    39601 gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg
    39661 tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata
    39721 ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca
    39781 actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag
    39841 tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg
    39901 agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc
    39961 gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg
    40021 agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac
    40081 gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg
    40141 acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca ttaaccgcca
    40201 gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat
    40261 tcactgtcga actggcagga aacgccgtct catttcgtat tgcgacctta ccatgtcggg
    40321 gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca
    40381 cgctgggaat gcagccgtta caactggcgg gctttgctca tgccttgcaa caaccacagg
    40441 gactggtgct ggtaactggc cctaccggca gcggcaaaac ggtcacgctt tatagtgccc
    40501 tgcaaaagct gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc
    40561 ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cggactcacc tttcagggcg
    40621 ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg
    40681 gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc
    40741 tacacactaa ttccacctgc gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct
    40801 ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgc aaactttgcc
    40861 cacattgtcg ccggcagcaa ggggagccca tccacattcc agtcaatgta tggccgtcgc
    40921 cgctgcccca ctggcaagca cccggttgtg tacattgcta ccacggtttt tatggtcgca
    40981 ctgccttatt tgaagttctg cccataacac cggtcatacg tcagcttatt tccgctaata
    41041 ccgacgttga atcgctggaa acgcacgccc gacaggcggg tatgcgaacg ctttttgaaa
    41101 acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtattgg
    41161 ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat
    41221 gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa
    41281 cagatggtta ccccactaag cctgaagcga attgctatca attctgcgca gtggcgagga
    41341 gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt
    41401 tctgaagggc tggcactgct ggcggaacag catcccagta agcaatggca agcgttgctg
    41461 caatctctgg cacacgatct cgaacagggc attgcctttt ccaatgcctt attaccctgg
    41521 tcagaggtat ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag
    41581 ctggatgaat gctgctttga actggcgcgt cagcaaagag cccagcgtca gttgaccgac
    41641 aaagtaaaat cagcgttacg ttatcccatc atcattttag cgatggcaat catggtggtt
    41701 gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc
    41761 ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc
    41821 tggctgctgg tgttgttcgg cattatgctg acgatagcca ataagttgct gatgcgccgc
    41881 ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat ggggtcactg
    41941 atgcggggac aaaaactcac gcagatcttt acgattctgg cgctgactca aagtgcaggc
    42001 attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg
    42061 caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgtta
    42121 aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacggg agaggcctcc
    42181 ggctcgctgg atctcatgtt agacaacatc gcccatcatc atcgggataa cacaatggcg
    42241 ctggcggata acctagcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt
    42301 atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga tgcgatgagt
    42361 gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac aggttgttga
    42421 agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc agttctttca
    42481 ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg
    42541 tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct gcgcgatatt
    42601 cagcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca
    42661 taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca
    42721 tgccgccaag catgacgaaa tcggcaccgc caccaaaggc tttcgccaca tcgcccggcg
    42781 tggtgcagcc accatcgctg atgatcattc cgcccagacc gtgcgcagca tcggcacatt
    42841 cgattaccgc agaaagttgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag
    42901 aacctgggcc aatgccaact ttaacgatat cggcacctga gaggataagc tcctcacaca
    42961 tttcaccagt cactacgtta ccagcacaaa tggttttggt cggccacgct tcacgcgctt
    43021 tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacgtcaata caaacgaagt
    43081 ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtac
    43141 cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc
    43201 actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag
    43261 aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc
    43321 agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt
    43381 taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt
    43441 caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt
    43501 gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc
    43561 tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg
    43621 tgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgagg
    43681 tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc caatgcgttt
    43741 gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca
    43801 ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaatatgat tgctgctgat
    43861 ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac
    43921 tggcttaacg ccctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa
    43981 gctacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa
    44041 aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg
    44101 cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc
    44161 gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc
    44221 gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca
    44281 caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc
    44341 gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg
    44401 accacgctgg cgcgctgcat ttcttccgta atgttagtga attactggat gttttcgagc
    44461 gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt aaactccaga
    44521 cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa
    44581 aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc
    44641 gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt
    44701 tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgactgc caggtagaaa
    44761 cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc
    44821 gtcagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg
    44881 gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt
    44941 ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg
    45001 taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta
    45061 ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc
    45121 ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa
    45181 aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc
    45241 agtgacattt gtctgatgcc gcacgtaggc ctgataagac gcggacagcg tcgcatcagg
    45301 catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacatacac acgcttaagc
    45361 ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc gactagcgac
    45421 atccacttac cgggttgccc ttctttaccc cacggctccc cttcccagct ttcgaccagc
    45481 cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa
    45541 aaatgttgtg gggtaatccc gacttcttcc tgaagttcac gcaccaccgc ctgttccggc
    45601 gtttcaccca tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct
    45661 gctgcgcgac gcgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt
    45721 tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt
    45781 caacagttag cttttattgc aggcggccat ggcactgctt gtatttttta ccagaaccgc
    45841 acgggcaagg atcgttacgt cctactttgc gctcaccggt ttgcgccgcc agtgcagctg
    45901 cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgcgctaaa cgctcggctt
    45961 ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc
    46021 tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg
    46081 attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct
    46141 gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca
    46201 tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat
    46261 acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt
    46321 ctttatccag ccactcagca attggtaaat cgaggtcgaa atcgttcttc agacgttcct
    46381 gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg
    46441 tcgctttgaa cacatcttca cgaatgctgt taatggtttc gctcacatcg ctgacatcca
    46501 gcaattcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt
    46561 ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa
    46621 tcgctttagt cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca
    46681 tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt
    46741 agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga
    46801 tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct gccagtaccg
    46861 catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt
    46921 ccagcgcggc aacttctgcc tgccagctac caccgagcac aatatctgta ccacgacccg
    46981 ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt
    47041 cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt
    47101 ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg
    47161 cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga
    47221 ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca
    47281 gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca
    47341 gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt
    47401 tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgac
    47461 ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga
    47521 tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca
    47581 tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg ccctctttca
    47641 ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt
    47701 cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga
    47761 tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc
    47821 cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca
    47881 gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccata ttgtcgcgca
    47941 ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc
    48001 gctttgccgg tgctggcatg cccggcaggt tgataccgac agtcaggcca aggaattcaa
    48061 acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacat
    48121 gcacgccttt accggttagt gcgttcagat aagcaggcag cgttgcggtc agggttttac
    48181 cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact
    48241 gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgtacc acggcgaaag
    48301 cttccgggat cagattttcc agcacttcgc ctttttccag acgtgcacga aactctgcgg
    48361 ttttcccttt cagttcttcg tcggagagtt tttccatctc cggttccatg gcattgatga
    48421 tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag
    48481 ttaataattt gattagcata ataaaatctc aaacgccccg cgttgcggag ttaataaaat
    48541 gaagtaaagg tttattgttg ttagctgagg cgttgagggc cagcacggat gccttgcgcc
    48601 tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg
    48661 cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt
    48721 aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag cgtttgcggt
    48781 gccattgcga aggaaagatg acggattacc gtgcgaatgg catgttgatg ccagtaatct
    48841 acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag
    48901 ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg
    48961 gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa ccatccctaa taagagatgc
    49021 ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg
    49081 ccatcccgat cgtattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc
    49141 cacagcacga atgaagataa gttgtccgta aaagtgctta agaaaacagc gt
//
LOCUS       DAZFKY010000011        48544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFKY010000011 DAZFKY010000000
VERSION     DAZFKY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            186..1403
                     /gene="alaA"
                     /locus_tag="ACHKCL_000587"
     CDS             186..1403
                     /gene="alaA"
                     /locus_tag="ACHKCL_000587"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5761629.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1487..2086
                     /gene="yfbR"
                     /locus_tag="ACHKCL_000588"
     CDS             1487..2086
                     /gene="yfbR"
                     /locus_tag="ACHKCL_000588"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5761630.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2145..3977)
                     /locus_tag="ACHKCL_000589"
     CDS             complement(2145..3977)
                     /locus_tag="ACHKCL_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5761631.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4064..4714)
                     /gene="hxpA"
                     /locus_tag="ACHKCL_000590"
     CDS             complement(4064..4714)
                     /gene="hxpA"
                     /locus_tag="ACHKCL_000590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5761632.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4725..5219)
                     /locus_tag="ACHKCL_000591"
     CDS             complement(4725..5219)
                     /locus_tag="ACHKCL_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5761633.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5302..5757)
                     /gene="yfbV"
                     /locus_tag="ACHKCL_000592"
     CDS             complement(5302..5757)
                     /gene="yfbV"
                     /locus_tag="ACHKCL_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5761634.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6095..7297
                     /gene="ackA"
                     /locus_tag="ACHKCL_000593"
     CDS             6095..7297
                     /gene="ackA"
                     /locus_tag="ACHKCL_000593"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5761635.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7372..9516
                     /gene="pta"
                     /locus_tag="ACHKCL_000594"
     CDS             7372..9516
                     /gene="pta"
                     /locus_tag="ACHKCL_000594"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5761636.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9706..11226
                     /gene="yfcC"
                     /locus_tag="ACHKCL_000595"
     CDS             9706..11226
                     /gene="yfcC"
                     /locus_tag="ACHKCL_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5761637.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11259..11801)
                     /gene="yfcD"
                     /locus_tag="ACHKCL_000596"
     CDS             complement(11259..11801)
                     /gene="yfcD"
                     /locus_tag="ACHKCL_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5761638.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11859..12410)
                     /gene="yfcE"
                     /locus_tag="ACHKCL_000597"
     CDS             complement(11859..12410)
                     /gene="yfcE"
                     /locus_tag="ACHKCL_000597"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5761639.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12466..13110)
                     /gene="yfcF"
                     /locus_tag="ACHKCL_000598"
     CDS             complement(12466..13110)
                     /gene="yfcF"
                     /locus_tag="ACHKCL_000598"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5761640.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13246..13893
                     /gene="yfcG"
                     /locus_tag="ACHKCL_000599"
     CDS             13246..13893
                     /gene="yfcG"
                     /locus_tag="ACHKCL_000599"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5761641.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13950..14312
                     /gene="folX"
                     /locus_tag="ACHKCL_000600"
     CDS             13950..14312
                     /gene="folX"
                     /locus_tag="ACHKCL_000600"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5761642.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14333..15226
                     /locus_tag="ACHKCL_000601"
     CDS             14333..15226
                     /locus_tag="ACHKCL_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5761643.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15274..16164)
                     /gene="rpnB"
                     /locus_tag="ACHKCL_000602"
     CDS             complement(15274..16164)
                     /gene="rpnB"
                     /locus_tag="ACHKCL_000602"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5761644.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16361..17134)
                     /gene="hisP"
                     /locus_tag="ACHKCL_000603"
     CDS             complement(16361..17134)
                     /gene="hisP"
                     /locus_tag="ACHKCL_000603"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5761645.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17142..17858)
                     /locus_tag="ACHKCL_000604"
     CDS             complement(17142..17858)
                     /locus_tag="ACHKCL_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5761646.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17855..18541)
                     /gene="hisQ"
                     /locus_tag="ACHKCL_000605"
     CDS             complement(17855..18541)
                     /gene="hisQ"
                     /locus_tag="ACHKCL_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5761647.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18631..19413)
                     /gene="hisJ"
                     /locus_tag="ACHKCL_000606"
     CDS             complement(18631..19413)
                     /gene="hisJ"
                     /locus_tag="ACHKCL_000606"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5761648.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19634..20416)
                     /gene="argT"
                     /locus_tag="ACHKCL_000607"
     CDS             complement(19634..20416)
                     /gene="argT"
                     /locus_tag="ACHKCL_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5761649.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20682..21251)
                     /gene="ubiX"
                     /locus_tag="ACHKCL_000608"
     CDS             complement(20682..21251)
                     /gene="ubiX"
                     /locus_tag="ACHKCL_000608"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5761650.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21346..22863)
                     /gene="purF"
                     /locus_tag="ACHKCL_000609"
     CDS             complement(21346..22863)
                     /gene="purF"
                     /locus_tag="ACHKCL_000609"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5761651.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22900..23388)
                     /gene="cvpA"
                     /locus_tag="ACHKCL_000610"
     CDS             complement(22900..23388)
                     /gene="cvpA"
                     /locus_tag="ACHKCL_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5761652.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23829..24491)
                     /gene="dedD"
                     /locus_tag="ACHKCL_000611"
     CDS             complement(23829..24491)
                     /gene="dedD"
                     /locus_tag="ACHKCL_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5761653.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24481..25749)
                     /gene="folC"
                     /locus_tag="ACHKCL_000612"
     CDS             complement(24481..25749)
                     /gene="folC"
                     /locus_tag="ACHKCL_000612"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5761654.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25819..26733)
                     /gene="accD"
                     /locus_tag="ACHKCL_000613"
     CDS             complement(25819..26733)
                     /gene="accD"
                     /locus_tag="ACHKCL_000613"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5761655.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26889..27548)
                     /locus_tag="ACHKCL_000614"
     CDS             complement(26889..27548)
                     /locus_tag="ACHKCL_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5761656.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27631..28443)
                     /gene="truA"
                     /locus_tag="ACHKCL_000615"
     CDS             complement(27631..28443)
                     /gene="truA"
                     /locus_tag="ACHKCL_000615"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5761657.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28443..29456)
                     /locus_tag="ACHKCL_000616"
     CDS             complement(28443..29456)
                     /locus_tag="ACHKCL_000616"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5761658.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29522..30658)
                     /gene="pdxB"
                     /locus_tag="ACHKCL_000617"
     CDS             complement(29522..30658)
                     /gene="pdxB"
                     /locus_tag="ACHKCL_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5761659.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30757..31752
                     /gene="flk"
                     /locus_tag="ACHKCL_000618"
     CDS             30757..31752
                     /gene="flk"
                     /locus_tag="ACHKCL_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5761660.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31749..32927)
                     /locus_tag="ACHKCL_000619"
     CDS             complement(31749..32927)
                     /locus_tag="ACHKCL_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761661.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33202..34422)
                     /gene="fabB"
                     /locus_tag="ACHKCL_000620"
     CDS             complement(33202..34422)
                     /gene="fabB"
                     /locus_tag="ACHKCL_000620"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5761662.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34581..36587
                     /gene="mnmC"
                     /locus_tag="ACHKCL_000621"
     CDS             34581..36587
                     /gene="mnmC"
                     /locus_tag="ACHKCL_000621"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5761663.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36708..36986)
                     /locus_tag="ACHKCL_000622"
     CDS             complement(36708..36986)
                     /locus_tag="ACHKCL_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5761664.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37020..37568)
                     /gene="epmC"
                     /locus_tag="ACHKCL_000623"
     CDS             complement(37020..37568)
                     /gene="epmC"
                     /locus_tag="ACHKCL_000623"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5761665.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37568..38377)
                     /locus_tag="ACHKCL_000624"
     CDS             complement(37568..38377)
                     /locus_tag="ACHKCL_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5761666.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38377..39201)
                     /gene="mepA"
                     /locus_tag="ACHKCL_000625"
     CDS             complement(38377..39201)
                     /gene="mepA"
                     /locus_tag="ACHKCL_000625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5761667.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39205..40290)
                     /gene="aroC"
                     /locus_tag="ACHKCL_000626"
     CDS             complement(39205..40290)
                     /gene="aroC"
                     /locus_tag="ACHKCL_000626"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5761668.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40325..41257)
                     /gene="prmB"
                     /locus_tag="ACHKCL_000627"
     CDS             complement(40325..41257)
                     /gene="prmB"
                     /locus_tag="ACHKCL_000627"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5761669.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41423..41974
                     /gene="smrB"
                     /locus_tag="ACHKCL_000628"
     CDS             41423..41974
                     /gene="smrB"
                     /locus_tag="ACHKCL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5761670.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42145..42987)
                     /locus_tag="ACHKCL_000629"
     CDS             complement(42145..42987)
                     /locus_tag="ACHKCL_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5761671.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(42989..43510)
                     /locus_tag="ACHKCL_000630"
     CDS             complement(42989..43510)
                     /locus_tag="ACHKCL_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761672.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(43507..43977)
                     /locus_tag="ACHKCL_000631"
     CDS             complement(43507..43977)
                     /locus_tag="ACHKCL_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761673.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(43974..44474)
                     /locus_tag="ACHKCL_000632"
     CDS             complement(43974..44474)
                     /locus_tag="ACHKCL_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761674.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(44485..45243)
                     /locus_tag="ACHKCL_000633"
     CDS             complement(44485..45243)
                     /locus_tag="ACHKCL_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5761675.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(45266..47905)
                     /locus_tag="ACHKCL_000634"
     CDS             complement(45266..47905)
                     /locus_tag="ACHKCL_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5761676.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQGTLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(47987..>48544)
                     /locus_tag="ACHKCL_000635"
     CDS             complement(47987..>48544)
                     /locus_tag="ACHKCL_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5761677.1"
                     /translation="KFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCSIV
                     PDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTNSG
                     NYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDFKA
                     WLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11903 a        13091 c        12132 g        11418 t
ORIGIN      
        1 ttgcatgtaa agcatatgca cctcattatt ttgtcattat taagttatta acagcacaat
       61 cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
      121 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
      181 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
      241 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga
      301 acatcggcaa cccagccccg ttcggttttg acgcgccaga tgaaatcctc gttgacgtga
      361 tacgcaacct gcctaccgct caagggtatt gcgattccaa aggtctttac tccgcgcgta
      421 aagccatcat gcagcactat caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
      481 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
      541 gggacgaaat gttggttcct gcaccagatt acccactatg gaccgcggcg gtttcgcttt
      601 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
      661 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
      721 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
      781 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgacgctg
      841 agcatcactc aattgcgccg ctggcacctg acctgctgac cattaccttt aatggactgt
      901 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
      961 aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
     1021 acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat
     1081 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ttgatcaacg
     1141 atattccggg cgtttcctgc gtgaaacctt gtggtgcgct gtacatgttc ccgaaaatcg
     1201 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
     1261 aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
     1321 ttgtcacgct accgcgtgtc gatgatatcg agctgtcgtt gagcaagttc gcgcgtttcc
     1381 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagtgt
     1441 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
     1501 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctca tgcgcaacgt
     1561 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
     1621 agctatcaaa aatcggaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
     1681 gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt
     1741 caattcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt
     1801 cgatatggtt ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata
     1861 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
     1921 tctggaagaa ctcgcggcgg gaaataatga attcttgctg gcaaaaacgc gactggaagc
     1981 gacgcttgaa gcgcgccgca gccaggagat ggactacttc atggaagtat ttgttcccag
     2041 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ctggagtctg
     2101 cgtcgcatca ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc
     2161 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccagcttc
     2221 acaaagtcac tgaagctgta atttcccgga cctaaaacca atgtgttaac aggtgaagaa
     2281 accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc
     2341 gacaccccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
     2401 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaacc cagcatcata
     2461 tgtggcccgt aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
     2521 gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag
     2581 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
     2641 gcgatgatag cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc
     2701 accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
     2761 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
     2821 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
     2881 tccagcgcca cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa
     2941 ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc
     3001 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
     3061 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
     3121 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
     3181 cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc
     3241 agccgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
     3301 agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg
     3361 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
     3421 agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc
     3481 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgcta
     3541 atcagcccgg caaaacttaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg
     3601 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
     3661 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca
     3721 actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca
     3781 ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccaga aaatacttct
     3841 gggactgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
     3901 atacgcactc tgcccgtcgc aaacaagacg atggcaacgg ccagtaatga aagaacccaa
     3961 atcaattcac cgttcacaac ttatccttgt taattgaggg ggatgacttg attctgccat
     4021 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
     4081 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct
     4141 cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatggcaa cccgccgcca
     4201 ggccagaaag cacgccagcg ggagcatctt ccaccaccac acactcctgc ggcgcaagcc
     4261 ccagcagctg cgcgcctaac agatacgcat caggttctgg ttttccgcgc ttcactcgct
     4321 cagcggttac aaacacctct ggtgcgggaa gccccgctat tttatggcgc gctcgcgcta
     4381 ccggcatgga gccagaagtc acaatggccc acggaatacc tgctttattc aaatgattga
     4441 gtaaggcgat tgcccccgga agcgcggtaa taccttcggt ttccgtggcc tcgatttgct
     4501 ccaaacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata aaatggcgca
     4561 gagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc gctaacccat
     4621 gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc accagcgttc
     4681 catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc gttgataatt
     4741 tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca cacattaagc
     4801 atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccgtgagt tccggcgtca
     4861 aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag gtaacgtgct
     4921 tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga ttgctggtcc
     4981 tgcaaattag accaggaaac atgcaacgca tgatacatct ccataatgtc gatgatagtg
     5041 cggcaggttt cttctttcag ctcgccaaac tcgcgatcca gttcacgcat ctgtaatcca
     5101 taaccccgct caatgattgt ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc
     5161 atagtcatca tcttgtactg gttagacaaa ataagacgtt gcgcgttggt catttccatt
     5221 gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat gccttctttt
     5281 atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca gttgtttgaa
     5341 ggcacgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa
     5401 cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttga ggattgcagg
     5461 gggtaatggc gtgacagaac gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa
     5521 ggcgaacagg gcagtggcaa ctgccggccc aagctgaccg cccagggcaa tctgccagca
     5581 gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca tcttgataac
     5641 gcgattttcg acaaagaccg gggcaaggcg tttttccagc ggccacgtct ttgagtaatg
     5701 ctgtccccgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg ttgacatgct
     5761 tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac tcagcgggac
     5821 aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc agcctgaagg
     5881 cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca ttggctgaaa
     5941 attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt
     6001 gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg
     6061 tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag tactggttct
     6121 gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg gtgaagagta
     6181 cctttctggt ttagccgaat gtttccacct gcccgaagca cgtatcaaat ggaaaatgga
     6241 cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag cgctcaactt
     6301 tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga ctgctatcgg
     6361 tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg atgagtctgt
     6421 tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg ctcacctgat
     6481 cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg ttgctgtatt
     6541 tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc tgccgtacaa
     6601 cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt
     6661 aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca tcatcacctg
     6721 ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc
     6781 tatgggcctg accccgctgg aaggtctggt catgggtacc cgttctggtg atatcgatcc
     6841 ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa tcaacaaact
     6901 gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact gccgctatgt
     6961 tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt actgccaccg
     7021 cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg acgctgttgt
     7081 attcaccggt ggtatcggtg aaaatgccgc gatggtccgt gaactgtctc tgggcaaact
     7141 gggcgtgctg ggctttgaag ttgatcatga acgcaatctg gctgcacgtt tcggcaaatc
     7201 tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca acgaagaact
     7261 ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca gctcagctgg
     7321 cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt
     7381 attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg
     7441 atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg
     7501 cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa ctcttccacc
     7561 acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc cagcaatcag
     7621 aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa agacgctgaa
     7681 gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc aatttgccca gtctctgaac
     7741 tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca gggcactgac
     7801 accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg cggtgccaaa
     7861 aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga tgaacagggt
     7921 cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa agtaaacaat
     7981 gttgatccgg cgaagctgca agaatccagc ccgctgccgg ttctcggcgc tgtgccgtgg
     8041 agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc
     8101 atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg cgcacgcagc
     8161 attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt
     8221 cctgacgtgc tggttgccgc ttgcctggct gccatgaacg gcgtagaaat cggtgccctg
     8281 ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga acgtgctttc
     8341 gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacttc tctgagcctg
     8401 cagagcttca acctggaagt tccagttgac gatcacgagc gtatcgagaa agttcaggaa
     8461 tacgttgcta actacatcaa cgctgactgg atcgattctc tgactgccac ttctgagcgc
     8521 agccgtcgtc tgtctccgcc agcgttccgt tatcagctga ctgaacttgc gcgcaaagcg
     8581 ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc
     8641 tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga gatcaaccgt
     8701 gttgcagcgt ctcagggtgt agaactgggt gcaggcattg aaatcgttga tccagaagtg
     8761 gttcgcgaaa gctatgttgg tcgtctggtc gagctgcgta agaacaaagg catgaccgaa
     8821 accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat gctggaacag
     8881 gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac catccgtccg
     8941 ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt gttcttcatg
     9001 ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga tccgaccgca
     9061 gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcagcctt cggtatcgaa
     9121 ccgcgcgttg ctatgctctc ctactctacc ggtacttctg gtgcaggtag cgacgtagaa
     9181 aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt
     9241 ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc gccgaactct
     9301 ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc tgaacaccgg taacaccacc
     9361 tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct gcagggtatg
     9421 cgcaagccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt ctacaccatc
     9481 gcgctgactg cgattcagtc tgcacagcag cagtaacctc gtcatcatcc gcagctttgc
     9541 gctgcggata tctgaaccgg aaatagtcac tatttccggt tttttattct cttaatttgc
     9601 attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc gccatctcac
     9661 tttgcatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact
     9721 gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat cttttttgtt
     9781 gctattttaa ccagccttgc cacctgggta gttccggtgg ggatgtttga cagtcaggaa
     9841 gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc acactcattt
     9901 cgccttctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct gttcacgacg
     9961 ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag ggttaacctc aggatcgaaa
    10021 tacgggacag ccgttggcat catcatgttt atgctggtga ttggcggcgc gtttggcatt
    10081 gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca tacccgtggg
    10141 aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg cgcggtattt
    10201 ggtatggggg aagaggccgt cgcctttgcc attatcatcg caccgctaat ggtccggctg
    10261 ggctatgaca gtattaccac cgtcctggtg acctatattg ccacgcaaat cggttttgcc
    10321 agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg ggattgccgg cgttccggtg
    10381 ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactctgat tggcctgatc
    10441 tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag
    10501 tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt tacctttggt
    10561 gactggctgg tattgattgt cctgaccgcc gtaatggtct gggtgatttg gggcgtgatc
    10621 gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg tctggtgatt
    10681 ggcatcatcg gcgttgtttt ccgccttaac ggtatgacgg ttaataccat ggcttcatcc
    10741 ttcaccgaag gggcgcgaat gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg
    10801 attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca gcgtgttaaa tactatcctc
    10861 aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg gtttatgttg
    10921 ctcttccagg cggtatttaa tttcttcgtg acgtccggtt ctggtcaggc ggcgttaacc
    10981 atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt taccgtgctg
    11041 gctttccagt ttggtgatgg cttcagtcac atcatttacc cgacctcagc ttcgttaatg
    11101 gcgacgctcg gtgtttgccg ggtggacttc cgtaactggc tgaaggtggg cgcgaccctg
    11161 cttggactgc tgtttattat gtccagcgtc gtggtgatcg gcgctcagtt gatgggctac
    11221 cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt ttcagtctct
    11281 acggcttcat ttttggcatt gcgcttcatc cacaacgcta gcgctttcag cgagtctgga
    11341 gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac ttcactgact
    11401 tcatcttcct gtagggcgaa gggaccgtga gagacgcagc tgaacaatgc gccccagaca
    11461 cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac accggcaatg
    11521 cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc ggcctggact
    11581 acaccgccag cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc
    11641 tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg atgacgcaga
    11701 cactgtgccc gcatttgctc ccggctggct tgtgcaatga cttcgttctc ttcattgaca
    11761 atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa accttctttt
    11821 tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat cgcgacctgc
    11881 gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc cagcatgcca
    11941 taactcgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa gtggaaaatc
    12001 tccccccgct gttctgctac tggcagatgg gtatgaccgt aaaccagcac atcgttctgg
    12061 tttaaagcag gcagattttc cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt
    12121 ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcag catttgatcc
    12181 acttcgctgt cgcagttgcc gcgcacagcg ataaccttat gtgccacttc attaagccgt
    12241 tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac
    12301 acgtcgccaa ggatcaccag ccactgggca ccgctttggg caaacaactc cagaacacgt
    12361 tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat cactcactcc
    12421 tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc cagattgctt
    12481 cgctgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg ttgcataatc
    12541 caccaggcat tctggcacct catcgccatg tagtaccagg cggttaatca ttagcgccag
    12601 atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagta ctaacaaatg
    12661 ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta gtggcgcttt
    12721 tttcgccccc gcaaagacaa catctgtcgg acgctcttcg cggatgggca tcagatcgct
    12781 gcgcaaccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat
    12841 acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg cagaagattc
    12901 actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg
    12961 ccacgtcggt tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat gaaacaacag
    13021 gcctttttcc tgcaacgcca cccaagcgga taacacataa ggggagaaaa aatgggcatc
    13081 tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg gggctggcta
    13141 tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa gtcgccgcgc
    13201 tttctataat tgtggtgttc agatgattag acaaccggag ctccaatgat cgatctctat
    13261 ttcgccccga cacccaatgg ccacaaaatt acgctgtttc tcgaagaagc agggctggat
    13321 tatcgcttga taaaggtaga cctggggaaa ggcggtcagt ttcgcccgga atttttgctc
    13381 atttcgccta acaacaaaat tccggcaatt gttgatcatt ctccagccga tggcggcgaa
    13441 ccgctaagcc tctttgaatc tggtgccatt ttgttgtatc tggctgagaa aacggggctc
    13501 tttttgagtc atgaaacgcg tgaacgcgcc gccacattac agtggttatt ctggcaggta
    13561 ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc cccccaaacc
    13621 attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctgtacca tgtactgaac
    13681 aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc ggatattgcc
    13741 tgctggccgt gggttaatgc ctggactcgc cagcgaatag acctcgctat gtatccggca
    13801 gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc actgctaaaa
    13861 gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt gtattatttc
    13921 atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt attcgtataa
    13981 agaaccttcg tttacgtaca tttatcggaa ttaaggaaga agaaattaac aaccgtcagg
    14041 atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact agcgaagata
    14101 tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat gtagagaata
    14161 accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca cgtgaacatc
    14221 actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc tacgccgatt
    14281 cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct gcatgaacat
    14341 agtgatcacc ggagggacgg gattaattgg tcgccatttg attccacgtt tgctggagct
    14401 gggccatcaa atcacggtaa tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc
    14461 tcgggtgaca ctatggcaag ggcttgccga tcaaagcaac ctcaacggca ttgatgcggt
    14521 aatcaacctg gccggagaac cgattgctga taaacgttgg actcacgagc aaaaagagcg
    14581 tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta atgccagcga
    14641 cacgccaccg tcggtactca tttccggctc ggcaacgggc tattatggcg acttaggtga
    14701 agtggtggtt accgaagagg aaccgccgca taacaaattt acccataaac tctgcgcccg
    14761 ctgggaagaa attgcctgtc gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgtac
    14821 cggcgtagtg ctggcaccgg atggcggtat tctcggtaaa atgctgccgc cgtttcgtct
    14881 tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc atatcgatga
    14941 tatggtcaac ggcattctct ggctgctgga taacgagctg cgcgggccat ttaatatggt
    15001 ttcgccctac ccgatacgca atgaacaatt tgcccatgcg ctcggtcatg cgctgcatcg
    15061 cccggctatt ttgcgcgtcc ccgcaaccgc cattcggctg ttaatgggcg aatcttcagt
    15121 actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt
    15181 tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg gtttactgca
    15241 aacatccgcc agttaactcc cggtgttaca gggttagtgg ctttgcgcga taagatcgtc
    15301 tggtgaaagt cgggtcagcg tcaggatgac ttcgtggtca aatccttttt ccagcatctc
    15361 ctgagcaata cgcagagctt cttcgtgttt gccctgcatt gcgccttctt cacgtaatct
    15421 gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta tctcaccaat
    15481 aaatgcacgg aaacgctggg catctcccgt ttgtaataca taattaaaca gggcttttag
    15541 ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga ctaatcccaa
    15601 cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt tacgatgttg
    15661 cataatctcg tcatccggca ccacggtaat atccaccaac ggaaaagccg atgaatatat
    15721 tttacgggca atcgcaggct cggcaaattc atcaagccag cagagtgaat aaggataagg
    15781 acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt tatagcccac
    15841 atcaagatgg ttttgcattg ccgcaatgga ataacgcatc atgcgaaaag ccattaattc
    15901 ttccggctta ctttggtgct ctatcactac ataaagataa cccactccct cctgcgtttt
    15961 cacagaccac aagaggtcgg aataatattg ccgcaggtct tcatcaataa aactgtttgg
    16021 ttccagttta agcgtcgtta aatcacacag tttgcgcagc ggcgcgggaa gatgaatatc
    16081 aataaaatcc cgcgcggtgt ctggatggcg taaaaaagat ttaaataccg catcatgcgg
    16141 cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc tcaaatttaa gatgacgcga
    16201 caataaccac gattaacacc caatgcagca ggcagttttt atctttggac agcgaattca
    16261 gaagtatttt tagcggactg caacttttac atttcagcgg aagctgactc gcaaattccc
    16321 cttgaaaata ctccggcagc gttagccacc ggagaggcaa ttatttcagc gaacctttaa
    16381 ggaactgttg cagacgaggg ctttgcgggt tgccaaataa ctgctccggc gcgccttctt
    16441 cctctatttt cccctgatgg aggaaaatga cgtgagtaga tacatggcga gcaaagccca
    16501 tttcgtgggt caccaccacc atcgtttttc cctcttccgc cagctgctgc ataatgcgca
    16561 gcacttcacc caccagctct gggtcaaggg ccgatgtcgg ttcatcaaac aacagaactt
    16621 ccggttccat tgccagcgcc cgcgctatgg aaacacgctg ttgctgacca ccggacaggt
    16681 gcaccgggta tttcccctgc gcacgttcgt ctatcccgac ttttgccaga tacttcaccg
    16741 cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg aatcggcgct tccatgacgt
    16801 tttccagcac cgtcatatgg ctccagagat tgaagtgctg gaataccatc gtcaggcgtg
    16861 tgcgcagtaa gcgcagttga tttttatcgg cgactttgag ttgaccgtct ttgtcgcgca
    16921 ccagattgat cgtctggcca ttgaccacga tcgacccttc actcggtttt tcgaggaagt
    16981 taatgcagcg cagaaaggta cttttccccg atcccgacga tccgatgatg cttattacat
    17041 ctccggcatt cgcttgcagt gacacccctt tcagcacttc gtgttcgcca tagcgtttgt
    17101 gcaaatcgat aacgtttaat ttattctcgg acatcgtatt ctcagtgcgt tgaagaaggt
    17161 ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga ggctgatcag gacataagag
    17221 atgattaaat agagcaccgc ggcaatgccg aaggcggtaa aaggttgata cgtggcggcg
    17281 ttaatatcgc gggctatttt cagcagatcc ggcaccgtgg cagtaaatgc caacgcagta
    17341 gagtgcagca tcaggatcac ttcgttgctg tatgccggta acgcaatacg cagcgcagaa
    17401 ggcaaaataa tgcagcgata cattttaaaa gtcgagaagc cataggctct ggcggcttca
    17461 atttccccat gcggcaccga acggattgcc ccagcaaaaa tctcggtagt gtaagcgcag
    17521 gtgttaagcg tcagcgccag cacggtacag ttcaggccac tgcggaaaaa ggcgttaaga
    17581 aactcggtcc ccttaacaat ctccagcgta tacatgccgg aatagaacac cagcaactga
    17641 acatacagcg gcgtaccgcg aaaaatatag gtaaataacc agattggaaa ctggatgtat
    17701 ttattactgg agacacgacc aatcgccaga aacagcgcca ggactccgcc tatcactacc
    17761 gacaaaataa gcagccacag agtgatcgcc acaccagtaa agcgataacc gtcggtccac
    17821 agcagcggtt tccagtattc atgtaagatt tcgatcacag gtcagccctc ttcacaccca
    17881 cagagtagcg gcgctcaagg aacagcagca caccattgga aacggtggtg aaaaccaggt
    17941 aaatcacgcc acagacgata gcgaaataga acggttccca ggtacttttg cctgccagct
    18001 gcgtggcttt gaccacatct tccaggccga gtaacgaaac cagtgcggta gatttgagga
    18061 tcacctgcca gttgttgcca atgcctggca gggcgtaacg catcatcgcc ggaaacatga
    18121 tccgccgaaa cacttgccca cgagtaaaac cgaacgccct cgccgcctct atatgtcctt
    18181 tcggcactgc cataaaagca ccacggaacg tttcggtaaa ataagcaccg tagataaaac
    18241 cgagagtgat aataccagcg accatcggat cgatatcaat ctgcccgacg cccatcgcct
    18301 ccgtcaccgt gtttagcgca atctgcaaac cgtagaaaat cagcagcatc aacactaagt
    18361 ccggcacgcc acgaatcagc gtggtgtacc cttcgaaaat cagccccgaa agccgatttt
    18421 gcgagagctt accgccagcg ccaattaaac cgatgattac agcgagcact acagagctga
    18481 tagccagctc cagcgtgacg agcgcaccct gtaaaataac acctgaaaac ccatacaaca
    18541 tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt gtctccctcc ggttacccga
    18601 agggagcggg ctttctttca ccgatggcga ttaaccacca taaacatcaa aatcgaagta
    18661 ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt tcggcaaagg ctttgttcag
    18721 tgcctcgcgc agttcgttat cttctttacg caggcccatg ccggtcccta cgccaaacag
    18781 tttttcatct ttaacagacg ggccaccgaa tttgtaatct ttaccgacag gttgtttgag
    18841 gaaaccttcg ctggcagcga cctcatcctg gaacgcggca tcaatacgtc cggcagtcag
    18901 gtcagaataa atgttgtcct gcccctgata cgagacgatt tcaatgcctt ttggtgccca
    18961 atgttcatta ccgaacgtct cctgggtagt gccctgcaat acgcctaccc gtttgccttt
    19021 cagcgactcg actgtcggct gaatgtcaga atttttcgcc accaccagac gagaatcggc
    19081 agcgtacagt ttgtcggtga aggctatttc ttgctgacgt ttttccgtaa tggaaagcga
    19141 tgacatgatg gcgtcaatct tcttcgcttt taaggacggg attaacgcat ccagcggatt
    19201 ttcgacaaac gtacattgcg tattgatgcg tttgcataat tcctttgcca gatcgatatc
    19261 gaagccaacc agttcgcctt gtgaattttt tgattcaaat ggcgcatagg tcgggtcggt
    19321 accgatgcgg atgttttgcg gaatcgcagc aaatgccgca gttgcgctgg agaaggccag
    19381 aaccagagag agcgatagca ccagtttttt cataatgtcc tcaactgaca gtctttttat
    19441 agggaatatt tacaggtttg ttacacttat cgtgccataa aattgaccat caaggcaaaa
    19501 tatcccgccc cgttagacgt attatttttc attaaaaatg cttaagtatg catttcaatg
    19561 caccacgata gtgcaacaaa cctggcgtgc accatgatga tcattccatc aggtacagct
    19621 tccctgcgac gtatcagtca ccgtagacat taaagtcaaa atactttttc gccatcttgt
    19681 cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt gaaggcagcc gtcagttcag
    19741 catcatcttt acgtagccct acaccggtgc catcaccgaa gtattttttg tcttttactg
    19801 atgagccagc aaaggcgaaa tctttaccag caggttgctt gaggaatcct tcgctggcag
    19861 caacttcatc ctgcagtgca gcatccagac gtcctgcagc cagatcggaa tagaccaaat
    19921 cctggttggc ataggccacc acatccacac ctttgctacg ccaggtctcg ttagcgtaag
    19981 cttcctgggt tgatccctgc agcacaccaa catgtttacc tttcagtgag tccagcgttg
    20041 gctgaatcgg cgaacctttg gcggcaatca gacgggaatc tgcggcgtac agcttgtcgg
    20101 agaaggcaat ctcctgctga cgtttatcgg taatggaaag cgacgaaata atggcgtcga
    20161 tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc actggcaacc caggtacatt
    20221 tcacctgcat ccgtttgcac atctcgttac cgagatcgat atcaaagccg acaaagtcac
    20281 ctttggcatc tttcgacgag aacggtgcat aggtggtatc ggttccgata cgtaccgtct
    20341 ccggcaatgc cgcatagctg gaagccgctg cggaaatacc gactaacaaa gacagagcga
    20401 gaatcgactt cttcatacat aaccctcaag tgaaaatggc ttttttatgt tgtgtattgt
    20461 gttgtgtgtt tgcaggcttg ttcatgcagg tcttatgcca tcttgacgac aacagtaaca
    20521 ttcaacgtta aatatgttaa taagacgttg cattattgtc ctaaagttga agatagcagg
    20581 tatagcggtt gaatcgcagc gtttcgattg aatggcagaa acaaaatgtc gaggatttga
    20641 tcgcggttgc aaaattgccc tgaaacaggg caacagcgga gttatgcgcc ctgccagcgg
    20701 gcaaagagat cttcaggaag ggttatcgca aactggtcaa gaacacgatt aaccgtctga
    20761 tttatcacat catcaaggga ttgcgggcga tgataaaacg ccggaacggg aggcataatc
    20821 accgcaccga tttctgctgc ctgcgtcatt aaacgcagat ggcctaagtg caatggtgtt
    20881 tcacgcacgc agagcaccag tgggcgacgc tctttcagca ccacatctgc cgcacgggtc
    20941 agtaagccat cagtatagct atggacaatg ccggacaggg ttttgattga acagggtaaa
    21001 atcaccatcc ccagtgtctg gaaagaaccg gaagagatgc tggcggcaat atcgcgcgca
    21061 tcatgcgtga catcagctaa tgcctgcact tcgcgcaggg aaaaatccgt ttcgagggat
    21121 aaggtctggc gcgccgcctg gctcattacc agatgcgttt cgatatctgt gacatcgcgc
    21181 agaacctgta ataagcgcac accataaatc gcgccgctgg caccgctgat gcccacaata
    21241 agtcgtttca taaatagttg ccctgtcaga cttgcaggca gactttgcag gatttcggcg
    21301 ggagttgcaa gtcagggtgc cagaccggca ccctgagcga aggcatcatc cttcgttatg
    21361 catttcgaga ttttccactt cgttctgacg ttgcactgct ttggcgtcgt cattacgtaa
    21421 agtatcgagg aaatcgaggt agccctgatc aacatctttg gtgacgtaga cgccgttgaa
    21481 caccgagcat tcaaactgct ggatatccgg gttttcagcg cgaacggctt cgatcagatc
    21541 gttcagatcc tggaaaatca acccgtcagc accgatgatc tggcgaattt catctacttc
    21601 gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca ataccataaa cgttcgggaa
    21661 gcgaatttcc ggtgccgcag aagcgaggta cactttcttc gctccggctt cgcgtgccat
    21721 ctcgataatc tgctcagaag tggtgccacg gacgatagag tcgtcgacca gcaggacgtt
    21781 tttatcgcgg aactcggcgc ggttggcgtt cagtttacgg cgcacggact tacgacgcag
    21841 ctgctgaccc ggcatgatga aggtgcggcc aacatagcgg tttttaacga agccctggcg
    21901 gtacggtttg cccagaatac gagcaatttc cagcgcgata tcacacgagg tttccgggat
    21961 cgggatcacc acgtcgatat ccagatcttc ccattcgcgg gcaattttct cgcccagctt
    22021 cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg tcgatgaacg agtccgggcg
    22081 agcaaagtat acatactcaa acaggcacgg attgctgacc ggattgtcag cacattgacg
    22141 ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg ccaggcgcga cgtcacgcag
    22201 gaaatcaaag cccagtgtat cgagcgcaac gctttcggaa gcgaccatat attctgtgcg
    22261 gttctcgtca atatcacgtt ttcccagaac cagcggacga ataccgtttg gatcgcggaa
    22321 agcaaccata ccgtggccga taatcatcgc cacacaggca tacgcgccgc ggattaagcg
    22381 gtttgtagca gcgatggcgg cgaaaatgtt gtcggcttca agcgggtagt ggcggaagtt
    22441 gtccagctcg ctggcgaaga tattaagcag aatttccgag tcggaagtgg tgttgatgtg
    22501 gcggcgtttt tcttcaaaca gttttttacg caactcgtga gcgttggtca gattgccgtt
    22561 gtgggcaagc gtaatgccat acggagagtt aacgtaaaac ggctgcgctt cagaggcgct
    22621 ggagctgcca gccgtagggt aacgcacatg accaatgccc atattgccct gcaaacgctg
    22681 catatggcga gcttcaaata catcgctcac cagcccgttc gctttacgca aacggaagca
    22741 gttattggca tctatggtga tgatgccggc ggcatcctga ccgcgatgct gaagcaccgt
    22801 taaggcatca taaatcgact ggttaaccgg cataacaccg gcgataccga caataccgca
    22861 catacgtctt tttcctcgtt aagccacatc tcagagcact tacgctctgg gcaagaaact
    22921 tgacgagctt tgcagataat caaaaaacca tctgatgata aaactgaact gcggaatcag
    22981 ttgtgattta ctccagtctt cgcttttcga caccccggta aaggagtcga gaaagaagag
    23041 aatagcagca acaatcaaca caccgcgcaa tgcaccgaaa cagacaccca gcacccgatc
    23101 ggtgcctgac aaccccgttt tctccaccag caggcctatc acgaagttca cgatagcgcc
    23161 aacgatcagg gtggcgataa acaggaccgc gatggcaatc ccatttcgaa ccagttcgtc
    23221 ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa tgactggcaa caaagaaagc
    23281 acaaccccat gtcaccagcg ataacgcttc acgaacaaag ccgcggatca ggctaaccag
    23341 agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc cagaccatat gtgtcccacg
    23401 attttacgcc ctgtcatcct gttcggggcg cattctaaca gaaaaagaaa acgtttgcgt
    23461 agggatttcc ttcccgcgca tcaataaaaa tggcgctgaa aaaatattca acgccatcga
    23521 ctttttatgc ctttgcggca tcggacaatg cgtgtcggat gcggcgtaaa cgtcttatcc
    23581 gacctacggt tctacccctg cgtaggcctg ataagacgca ccagcgtcgc atcaggcaac
    23641 gcctgtcgga tgcggcgtga atgccttatc cgacctacgg ttctacccct gcgtaggcct
    23701 gataagacgc gccagcgtcg catcaggcaa cggctgtcgg atgcggcgta aacgccttat
    23761 ccgacctacg gttctacccc tgcgtaggcc tgataagacg cgccagcgtc gcatcaggca
    23821 agaccgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    23881 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    23941 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    24001 agcttaccga caatctcatt cactttatcg gcatttttca gcgcgcccag ttgcacaaca
    24061 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    24121 gcaggtggtg cttcaacctt cggcttaggc ggctccaccg gtttcggctt cggtgggacg
    24181 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    24241 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggttgcgtc
    24301 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttggc
    24361 accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    24421 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    24481 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    24541 cgaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    24601 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    24661 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    24721 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    24781 gtttttcggt agcgttttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    24841 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    24901 gctggcaatc ccgtcgcgaa tggcattttc actgacctcc agcccgctgg cacgcagtgc
    24961 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcaggt tttccagcgt
    25021 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    25081 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    25141 cggctcaccg acaattgccg gtttttcgct gcgaaagatg cctgctttct cgcgaccaat
    25201 actttcgcga tctggcccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    25261 gacatcggcg tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    25321 caccacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    25381 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    25441 cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacaca ccagatgagg
    25501 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    25561 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac
    25621 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    25681 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    25741 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    25801 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    25861 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    25921 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    25981 caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct gttcgataac
    26041 acgcggaccg gcaaagccga ttaacgcttt cggttcagcg atgttgagat cgcccagcat
    26101 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    26161 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    26221 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    26281 ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    26341 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    26401 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    26461 atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccagctc
    26521 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    26581 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    26641 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    26701 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    26761 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    26821 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    26881 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    26941 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    27001 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    27061 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    27121 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    27181 ttctcataaa actgatgggt tttatcgaga taactgcgac ggaaaatttt cgaattggga
    27241 ttactgaata acttttcacc gaacaagcgc ccgatagtgt aattgaccgc gtcacccaca
    27301 atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt
    27361 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cgttaccacc
    27421 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    27481 gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    27541 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    27601 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    27661 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    27721 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    27781 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    27841 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    27901 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    27961 gaaatcattc tcgcccagca agcactgcgc agcccgatgc atccgttcag cgtccagcgg
    28021 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    28081 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    28141 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca acgtccacgc
    28201 agcgtctttg cgctgcgcgg tagtttcgaa atgcacaacc tgcccggtac cgtgtacccc
    28261 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    28321 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    28381 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt gctggtcgga
    28441 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    28501 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    28561 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcacctac ctgagttggg
    28621 aattcgttct cttcagagag cacaatatct tccccttgaa caaacgcatc acgcgcttct
    28681 tctgctgcca gtggacgcag agcttcaaag ttgaccatct gggcatgacc gtagaatacc
    28741 ggtgcctgga cgacgcttgc cgaaatcatc agtccttcgt cctgcaggat tttgcgtact
    28801 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcag taacggcagc
    28861 atgttgaacg cgagctgacg cccgaagaaa tcttcttcgt caatcggaat gccgttgagc
    28921 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    28981 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    29041 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    29101 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc aagagcaaac
    29161 aatccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggctgcg
    29221 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagca
    29281 gcatcctgca cggtgattgt cttaccacca aagcgcaatt gttcgcctgc gctttcgtta
    29341 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    29401 gcttcgccca cagcgccagt tgtgcccagg acggcaatgt tccagccttc agacatggtg
    29461 gtttactcca gaaatagcaa agctccctgc caatgttata gcagagagca tgaagaagag
    29521 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    29581 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    29641 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    29701 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggtccatgca
    29761 gagtaatgcg accaaactct ggcgcaggca gtaatgtatc cagcgcaacg tgctgttcat
    29821 gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca
    29881 gggtatagcc tgcaatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattta
    29941 gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc
    30001 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    30061 cgggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg tacggcccgt
    30121 ctttgaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    30181 agcggaaatc accctcatcc ccacagtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    30241 tccctaacgc ttccagtcgt gcctgtaaac gccgaccaac gttaccaacg cctacgatcc
    30301 ccacggtacg ctcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    30361 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    30421 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt
    30481 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    30541 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    30601 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    30661 ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag
    30721 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    30781 cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    30841 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    30901 caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    30961 tcaccgctgc tttcgcgtca ctgccctgcc gctgagcata atctggcgca acgtctgctg
    31021 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    31081 ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    31141 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    31201 atggttattc cgcaaccgca gcagcgcgag gcgaccgatc gtcctctatt accggcggag
    31261 cacaatgcgc tcaaacagct ggtgaccaaa cttgcggcgg caacggggga gcccagcaaa
    31321 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    31381 aaactgttta accatctggt gacctggctg caggcgcgtc agacgctaag ccagcaaaat
    31441 acgccgacgc tggaatcact acaaatgacg ctaaaacaac ctttagatgc cagtgaactg
    31501 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    31561 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    31621 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    31681 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    31741 ctggttcgtt aaccccgacg aaacgacagt atcgtgacaa taattcccag caccgcagag
    31801 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgcaagc
    31861 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    31921 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    31981 catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcccctgg ggcttgccag
    32041 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    32101 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    32161 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    32221 ccgataaccg caaaaccaac gccttgtagt gccagaccta accctggttt ccagataagt
    32281 ccgacaacgc tccacagaga tggacgttct cccgccaggg ctggtacttt gcgcactgtg
    32341 ccgttacagg cccacgccag taagggtaat gccattgtgg tgagcgccag tgcggcaaaa
    32401 ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc accgtaaatc
    32461 gccattccat tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    32521 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    32581 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    32641 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    32701 tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg aatgccgacg
    32761 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacaa cgggataacc
    32821 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc
    32881 gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    32941 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacagtgcg
    33001 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg ggagtttgcc
    33061 tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    33121 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    33181 cctactccgg cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt
    33241 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    33301 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    33361 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgtacgccag cagcgcccag
    33421 agagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    33481 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtgccgt gggagttcag
    33541 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    33601 gccagaggga gcaaccatgt ctgcgccatc agacgttgcg ccgtagccaa cgatttcagc
    33661 atagatgtga gcaccacgcg ccagcgcgtg ttccagctct tcaaccacca ccataccgcc
    33721 gccgccagcg ataacgaaac cgtcacggtg agcatcgtaa gtacgggagg ctttttccgg
    33781 ggtgtcgttg tatttagtag acagcgcacc cattgcgtcg aattcgcaag ccatttccca
    33841 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    33901 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    33961 gccatgaatt ttaaacggcg tggcgaggca ggcagaaacg ccggatgcca tcgctttggt
    34021 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    34081 cacctggaaa cgcggggaac cgccgccaga acctgcaatc aggccaacac gcggattatt
    34141 ctggtaagct tccggagaga ggcccgcatc tgcgattgcc tgctccatag aaaggaatgc
    34201 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    34261 cagttttacg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    34321 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    34381 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    34441 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    34501 cagccaatta atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagctaccgc
    34561 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    34621 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    34681 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggca cgctttcctg
    34741 aacatccaca tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc
    34801 tgacgctatg gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    34861 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    34921 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    34981 ccttgcccgg ttgccatcgt ttattacttg atgaaggccg cgtgacgctg gatttatggt
    35041 ttggcgatat taatgaactg atcagccaac tggacgattc tctaaatcaa aaagtagatg
    35101 cctggtttct ggacggcttt gcgccagcga aaaacccgga tatgtggacg caaaatctgt
    35161 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    35221 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    35281 gacgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    35341 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    35401 ttgccagcgc attgttgtcg ctggcgctat tgcgtcgcgg ctggcaagta acgctttatt
    35461 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ttgtatccgt
    35521 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgggttt acttttgctc
    35581 gtcgacttta tgattcatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    35641 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    35701 aagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttacgacaa
    35761 attgcagcgg tattacttat ccgcaaggtg gctggctgtg tccggcagaa ctgacccgta
    35821 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacagg
    35881 atttatcccg taaggatgac tgttggttgt tgacgtttgc aggagatcag caagcgacac
    35941 acagtgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acatcgtctc
    36001 tgccagtgta ttcggttgcc ggtcaggtca gccatattcc gacgacaccc gaactggcaa
    36061 aactgaaaca ggtgctgtgc tatgacggtt atctcacgcc gcaaaatccg gcgaatcagc
    36121 accattgtat tggtgccagt tatcatcgcg gcagcgaaga gacggcgtac agtgaagaca
    36181 atcagcagca gaatcgccag cgattgattg attgtttccc acacgcacaa tgggcgaaga
    36241 cggttgatgt cagtaaaaaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    36301 tgccaatggt aggtaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgctgg
    36361 cggagcagaa agataaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgctg
    36421 ctttaggttc gcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    36481 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaat ccgaatcggt
    36541 tatgggtgcg gaaattgttg aaaggtaaag cggttaaggc cgggtaatct gctctcgcag
    36601 tgtttgccgg atgcggcgtg aacgccttat ccgacctacg tgtgacgtgt aggcctgata
    36661 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta ctgctgtgac
    36721 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    36781 tcaccagcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    36841 gagtggtcat caccactttc cagttctgcg atggctaatg taaggtggcc acgcaaatac
    36901 ccactggcaa acaactcatc atcactggca tggtcaacca taccgtcgat taatgccaga
    36961 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    37021 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    37081 ttttgtagtg ctttaatgaa acgtgccggg cgttccggaa tgccatttgt tagataatcc
    37141 atcacctgcg catgtactcg ccgttgaaac accacgcggt ccggttcgaa gtcgccttcc
    37201 agattgtcgc aactgacgtt aaacgggtat cccgccgcca cgcagaacaa ccagtcgagc
    37261 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tttgggcatc gcgaccgtcc
    37321 gggcaatacc agtagccgaa atcaacctgc tcgcggcgcg ctttcccggc aatacaccag
    37381 tgcgaaatct catgaattgc gctggcataa aagccgtggg caaagacgat tcggttatac
    37441 ggtacttccg catcagcggg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    37501 ttaaaatcat cggcaaagca gctattaaaa atttcaatca actgctcata gtggtgtgta
    37561 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcataaagta
    37621 atttggcact catcaccgcc gagacgataa cgatcatcgg gcggatcagt ttttgtcctt
    37681 tgctcaacac cagtcttgaa cccatgcgcg cgccaaggaa ctgccccacc agcatcacaa
    37741 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagtaaaccg ccgatgtttg
    37801 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagcg
    37861 taacaaaggc cagcgcgtaa aacgatccgg ctgccggacc aaagaatccg tcataaaaac
    37921 cgacgcagcc accggcaatt aacgcgaagg gcaaaccgta catccggcgc tggcgatctt
    37981 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    38041 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    38101 cgacaaaggt catggcaata ttaagtttct gatcgcttaa actaaccact ttgcggcgaa
    38161 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagag
    38221 cattagcggg agacatcccc gctgccatca atgccggaat ggtgagtaac ccaccgccac
    38281 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    38341 gcggggaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    38401 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    38461 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg gtggcggtaa
    38521 aggttgatct tcacactcca gactatcggc aggacaacgt aatcgtacat gcatatgcgc
    38581 acgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    38641 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    38701 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    38761 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccggcgtgt
    38821 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg cgtgaccgcc
    38881 gttgaaacgc ccaccagcag gcattcccat atcgccaatc aacaccgtac ccatgcccag
    38941 attgctcacc tgactactca ggcgctggat aaacatcacc aggtccgggt gaccgaaata
    39001 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    39061 gccgacgata caaccattgg aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    39121 ggttattttt tgccacggcg tcgctgccag gctgacgcta ctggcaagca gggccagcag
    39181 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    39241 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttctgctat
    39301 cggcaccgcg cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    39361 ttcgccaaag cggttaatgg tgcgccccgg cacggtaata ctggaggtcg gtttcagtgc
    39421 catatgagca atgatttgct gcccgctgct aattccgccg agaatgccgc ccgcatggtt
    39481 actctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccacgca gcgccaccac
    39541 gtcaaaacca tcaccaattt ccacgccttt caccgcgtta atgctcatta gcgcatgggc
    39601 gatgtcggca tccaggcgat caaagaccgg ctcaccaagc ccggcaggaa cgccactggc
    39661 aacaacggtg actttcgcgc caatagagtc gccctctttt ttcagcgcgc gcatcagttc
    39721 atctaacgcg tcgattttgt ccgggtccgg gcagaagaat ggattttgct cgacctgcga
    39781 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc gaatttcgat
    39841 gccaaatttc tcagcgagat attttttggc aatcgcccct gctgcaacgc gcatggcggt
    39901 ttcacgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    39961 ggtgtaatcg gcatggccag gacggaaaac gtccttaatc gcactataat cctgagaacg
    40021 ctgatcggta ttttcgatca acaaaccaat gctggtgccg gtagtaacac cttcaaaaac
    40081 accggagaga attttgacct gatctggctc acgacgctgg gtggtatagc gcgatgtccc
    40141 agggcgacga cggtcgaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    40201 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    40261 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    40321 ttacttaatc tttataaatc gcgaaatgtt cacgggccgc cagcagctgc gctttggtga
    40381 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    40441 attgttccat aagatgtacc atgctgttgc cgacttcaca aatcagtacg ccatcatcag
    40501 caagataatc tgccgcgttt ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    40561 atgccaggcc cagttccggc tcgtggcggt attcatttgg caggtcggac atatcttccg
    40621 catcgacata cggcgggtta gtgacaatca ggtcgtactg cactttcggc aaatcacgga
    40681 acagatcgga acgaatcggt atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    40741 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcatc gacttccgct tccgggaagg
    40801 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    40861 gttgcttgct gataagtccg gcaaatttat tgttgatcaa ttcaccaatc ggcgagcgcg
    40921 gcaccagcac gcgttcatcg acgtaaaatt catggccgca gaaccacgct ttgttggtca
    40981 ggtaagctac cggaatgcgt tcgttgacgc ggcggatcac gcgctcaaca atacgatgtt
    41041 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg aatatccagc ggcaggtaga
    41101 gcgacggcaa taccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    41161 agatatttgc cgcgctgaag cggctcaccg accagcgcaa catgtcctga atggtttgca
    41221 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    41281 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    41341 agtgaggaaa aatacgttta aaacgatcga ttgcgctacg agtcgggtaa actgtaggaa
    41401 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    41461 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacca ttgtccatcg
    41521 gccacagcgt aaaaaagtca gcgaagtgcc cgtcaaacgg ttgattcagg agcaggttga
    41581 cgccagtcac tatttctctg acgaatttca accgttatta aatgctgatg gcccggtgaa
    41641 gtatgtacgc cctggtgtag accattttga ggcgaaaaag ctgcgccgcg gcgattattc
    41701 gccggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    41761 cgcgttgatc gccgcctgtc gccgcgaaca tgtgttttgc gcctgcgtga tgcatggtca
    41821 cgggaaacat atattgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtcat
    41881 ggcgtttcac caggcaccaa aagagtatgg tggtgatgcc gcattgctgg tgttgattga
    41941 agtggaagag tggctaccgc cggagttgcc atgaatatta gggggcggat gcgttttgcc
    42001 atttcgccct gaatgctggt atcgaatgcc ggatgcggcg taaacgcctg atccggctta
    42061 tcaaaatgtg caaattcaat acgttgtgat tcagttgtag gcatgataag acgcgtcagc
    42121 gtcgcatcag gcatcaacgc acaactacag ttcattcagt acatactgga aacggcatgt
    42181 agaatcaatt ggctcacggc tactggtatt cacctgtacc acaatgtcgt gtagctttct
    42241 atccgtcaat ggttgacgtg agggggtgac tgtcaccttt gcattatcac aaccatacga
    42301 ggtaaaggtt aataagtcag aaacagcacg acaatatgcc cctctggaat caaaaatact
    42361 gccatcgcag agtttgccgc tttccatcag gtaggtcgaa agcgtagtcg tgattgtcga
    42421 accatcggat tgcaatcgca gcgctttatt atggctaaca taattatcac tccaggatat
    42481 atcaaacgaa gcgacgggaa ttgtcgggca aatagaatca tgaactgttg ggcttgtata
    42541 cgtaccaaat ccaaatgcct cagatactga ataaaatgag gcaatccacg gacaaccata
    42601 attatcagat tctacagtga cgttaaaagt catatatgca cagacaaaga cacgagtatt
    42661 gataccaggg caatttgagg tgtccgtacg tctataatca gtcaaaacca gtgtcggtgc
    42721 cgggccagat ccgctccagg atattaactt gagggtgtcg ccttttcttg cattggagtt
    42781 gtattgtccg tagcttactt ctcccggcgt cactaccgtg acatagaaat ataaactgcc
    42841 ggaagcagta acgtttttca tcgcataata aattttagta ctggaaaagc ttgcactcat
    42901 gagtttatca ccggcatagc actttggtgt ttctaaactg agagcaagta atatcagact
    42961 tgtcaaaaat atcgtccatt ttttcatttt aaaaatactc aaaagttacc gtcattgtgg
    43021 cggtgaaatc acccgatgta acatttcgtc catttacggt ctgtaaccag gcattaaaat
    43081 tgacactgtt atttccctga tttaatggaa atgaggcacc tgtggtactg ttaataggta
    43141 cagccgcccc tccggcagtt tcaatgccaa tcccaacacc cgttgcagaa gatgacgaat
    43201 caatcgacag aaatcctggt aagtcggtat cttctgttcc catcaaggtc accttgacat
    43261 taaacgccgt ggtgcttttg caatctttca actgaaaaac taacggtacg cgatctgact
    43321 gaccacgttg cattaaatcg ctggcagcaa ttgtggggaa atgaatttct gcaagtaagt
    43381 tgccattgat tacaggagta caggatttac caagcaaatt accggtaaaa tgcaggttat
    43441 cggcagctgg ggccgttccg ctgaacaata tcagaccact gataagcgcc cacgcacggt
    43501 tattcttcat tggtaatcca cctgtaacgt agcccatgct tcaaagtctg actcgggtaa
    43561 gtcgacaccg ctctttttta cgggtactgc cgttagtacc ggaagggatg tttcattcac
    43621 cactaagggc gtatgcagct taaaatcgct cccggcttgt ttgaggtgta ttcctaatcc
    43681 attgagatcc gtggctacgg ccgaatcgtc aaagtcactc tgcgtaccac tccaggtcaa
    43741 cgtcatttcc atttgagggt cacggacagc ggtatcgcag gtaatggtat acggcacgac
    43801 ctgttcgtaa ttgctaccgt taatgtcatc aatattcacg tcccgaaacg tcaactctat
    43861 attttgccca ttactaatag agcaattggg tggctgcact aaataaccat gaaaagagac
    43921 attcgagagc gccataaagc taaagctcca caacattaag agagagatcc gtttcattga
    43981 taatccacca caattgtggc actggcgcta aattccgccg ccgttagcga tacaccgctc
    44041 tgcacgacag gaaccgcttc cagagccagt gcgccagatg aaaagttaaa cggtaaccat
    44101 gcgccaggcg ttaaatccaa aatggagtga tcgcttactt tctgaatacg gataccaaaa
    44161 ccggcaatac ccgtatcgat taccgtttct ccgttaatta cggttgtggt tgcctgcata
    44221 tttaagcgca ggtcgtccat tgccagattg ttgcacacaa gcgagtagtc aatggggcgt
    44281 ttgtaactta ccccatcgat cttggaaatg aagacattac caaactccac gctcgcaccc
    44341 gttaccgtac agggcggcgg ggcatcaacg agcacacgca agaccatcgc aaccgattta
    44401 ctccccgcca ttacaggcgt gctcacaatg ctacacagca ataaccacgc ccaggccgaa
    44461 taagctcctt tcatgctatt gctcctatcc ctggcctttg ctgttttgct cttcgtccac
    44521 ctgacaggta tttcccgtac agcggaagaa cagcggcaaa cgcgcaccat aatcattcac
    44581 ataggtcagc accgggacgc tatccatttt gacttttaac ggcacagagg tttttggcga
    44641 tatcaccatg ggggaaaatc ccgctgccgg attgccattt ttctgcgcac tggcgttact
    44701 gataaccacg aagtacggcg tcgggttatt gacggtaaac tcctgaccac tgagggttaa
    44761 ggtcacctta tgctgccacg ggtttttcat gctgacgttt tccagcgcct ttggtcgcca
    44821 gaacaactta atgcgcgtct gcagcgctat ctgtagcgtg ttaggtttgt tcgatttcgg
    44881 cgggatctca cgcacgttga aatagaacac gctctcgcgg tcggcaggca gcttgttgat
    44941 atccggcatg ccctgtacct tcacctgacc gttcatcatg gaatcgatgc gctgcactgg
    45001 cggcaggacc gtcagtggcg agctaatcct gttaccctgt ccatcttcca tccagctttg
    45061 tgccagatac ggcattttcg ggtcgttatt gcgcagcgtg acgctgattg atttatcact
    45121 ttcgttaaaa atcaggcgtg tacggtccgg cgtgacagag gcctgtactg acaggctggc
    45181 ggcagttatc agtaacgcca gcgtcgttgc ggccgttctg gcagagcaaa atagattcgt
    45241 catggatcat tccttaatca cgttattaat tggctgatac cgatactggc gcttccgttg
    45301 gcatcacctg ctgggtctgc tcctggatca ccggcttaat ttcatgaggc atcgaaggag
    45361 atatcgcccc tgtttgctgg catggtaata acaggccatt gaacagatcg gctggcagcg
    45421 ggtcaggcag atgaatatcg cagtgggcca caccgcccca tgaaacgatc atatgctcgc
    45481 caggttttac gcccgcgagg tagacgttgc cgtcatcgtc aaccagaccg acgttctgcg
    45541 cgctgtcgtt tttcacttcc gcgccaaacg gcggataact gccatcttgc agacgcagca
    45601 ccgccatcgc tttttgcccg ctgatcaccg agaacttacg gtagccaatg gccccttcgg
    45661 taagcgtgcc ctgcaccacg gactgggtgg cttcggcgtt ttccggcagg ttgtttaggt
    45721 cgatatacgc ctggttgcgg tagtagttgt tgacgtctgc caccactgcc ttaccgaaca
    45781 tattggtgta aaccgccgcg ccatttcctt ccaccggaac accagccaca ccgtcggcat
    45841 caatcagcag acgcgtaccg cccatattct gggtacggtg gagcgcgcca ccttgtgcgg
    45901 tgagcgtcgc gccgccctgt aaggaaatac ccgccgaggt gtactgccct tcatggtagt
    45961 tagcgctgag atcgacctgc gccagcgatc cgtcgtggct gtaataaccg tcaacgctgg
    46021 agtgattgtc gctggtgcct acgttcaact ggtaatgggt tgcgtcatcg acacggctga
    46081 aataccccac ctggctgctg tccgaaccgc tgccgtagtt gccgttatag ctgatggtgc
    46141 tgctgtcacc ccacggcata ctgagcgata tgtacacacc tttatcggcc tggttgtcat
    46201 actcgtagcg gtatccggtc atggaaatgc tcatgttgcg gatactgccc aggttgaagt
    46261 agtgcgagag catgacgttg tagttggtct gttcgtcgcg atcccagtag gtatggcggg
    46321 tgtagttgag ataaacagac acaccggcat ccctgaagtt ctggttatag gtggcggtgt
    46381 acatctcttt gtcgttgccg gtgcgcacca tttcgctgtc gctggcatcg agatactcgc
    46441 tcatggtcat gaagttctct tccgagaagc ggtatccggc aaaagtgacg cggctgttca
    46501 gttcatcgaa gtctttggaa tagctcaggc gaaacgagtt gccgtccagt gaacctttcc
    46561 cgtaggcggt ctctttatcc agacgggtat gcgagtgggt gatatcaaag gccaccgcac
    46621 caaacacaga caggtcgcga ccgacgccaa gcgccgccga ctgatagtgt tcatctgcca
    46681 gcgccccgcc gtatagcgac cagccgttgg caatccccca ggaagcttcg ccgccggaga
    46741 agaaaccgcc ttccacgtgg tgcccccact cctgcgggcg gcccatcatc agtttatagc
    46801 gcacctggcc ggggcgagtc aggaacggca tcgaggcggt gttgatgtca tattcctgca
    46861 cctgaccgtt ctgttcttca atgcggatat gcagcgtacc ggagaccgaa tcgccaagat
    46921 cctggatgcg gaacggcccc gccgggacct gggtttcgta gatgacgcgg cccaactggc
    46981 tgacggtcac tttcgcggtg gtgtgcgcca cgccggagat atccggcgca tagccgcgca
    47041 gattcggcgg caacatttga tcgtcggtgc tgatactgcc acccacgtag ttaaagccgt
    47101 cgaaaatatc agaattcagg tagtcttcgc caaggccaag tttggctttt agcgacggta
    47161 aggctcgcca ggcgtagtag cggctccact cccagttttt ttgcgtgtca tcaccgttga
    47221 taacatcgtc atcattgctt ttactatgca aataatcagt ctgccagtcg gcacgaagac
    47281 gccatgcgcc gaggttcacc ccaaccgtcc cgttaccgct aatttcattg ctgtcatccc
    47341 cgccattttc ttcatgtcgt gtctgggcgg taatactgta gtcagcaatt aaaccagata
    47401 taccgtcatc ccagcgtgaa ggcggatccc agttgatgtc ggtatattca aggtaagcct
    47461 ggggtaatga aatgactaac gctgactgac tcaggtcagc tttaatatca atgccttcca
    47521 gttgaccggg tttcaggcat ttgccgtcgt ggatccattg caggtttttt gccacatcct
    47581 cttttaagcc aaactgcgcg accagttcag gcgtcaggca ggcataggtt ttactggcat
    47641 cgttctcaga ggcgtaccag taaatatcgt attcttccgt cagcggctgt ttatttagtt
    47701 gaacctgtaa attgtatttc ccaggttcga cataaccctg gctggaaaat cgcttcagat
    47761 cgatttttgt gtcgcctttt aactccagaa aacgggaatc aaactgaatt tcattttcag
    47821 cccaggcatt gacataactc ccggacattg ccaatgcaac gcagcaggga agtacccgca
    47881 gtcggaaaag agaatggtca ggcatacacg tatccatccg taattattat aatttgcgcg
    47941 aattccattt aaaaacccca tagcccgaaa gctatggggg tatcacttag agataagtaa
    48001 tctggaaggt cgtgttggct tcaaaattac ctaaatctgg cgcatcagca gcgcccacca
    48061 gccaggcttt aaagtccagc gtctgcttcg ctttgccttt gttggtcgcc gtatcgttta
    48121 cgattttctg ttcgataccc gcgccgcttt tataagaggt tccctgagcg tcaccaatgg
    48181 ccaggctgac attgttaaat gccgcaccag tatcggtatt gtaaatggtg tagtaattgc
    48241 cgctgttggt agaagacgcg ttaccggtga aggtagtggt catcgttgtc tgggtgtcaa
    48301 acacacagtc ttgcagatgg atctggaaag ctttcggtgt agaagtcccg ttatttttca
    48361 ggatccctga accaatgtca cccatatcaa cttccagttt gtgatcatca ggaacgatag
    48421 agcacgggga atcttcaatg gtgccgtaga aacgtgctgt accattgttg ccagcggcga
    48481 ttgttgaagt agaagcgaac gcacccatta ccattgttgc agcaatagct gtttttacaa
    48541 actt
//
LOCUS       DAZFKY010000012        46681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFKY010000012 DAZFKY010000000
VERSION     DAZFKY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            40..2466
                     /gene="ynfE"
                     /locus_tag="ACHKCL_000636"
     CDS             40..2466
                     /gene="ynfE"
                     /locus_tag="ACHKCL_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO5761678.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2527..4950
                     /gene="ynfF"
                     /locus_tag="ACHKCL_000637"
     CDS             2527..4950
                     /gene="ynfF"
                     /locus_tag="ACHKCL_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5761679.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4961..5578
                     /gene="ynfG"
                     /locus_tag="ACHKCL_000638"
     CDS             4961..5578
                     /gene="ynfG"
                     /locus_tag="ACHKCL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5761680.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5580..6434
                     /gene="ynfH"
                     /locus_tag="ACHKCL_000639"
     CDS             5580..6434
                     /gene="ynfH"
                     /locus_tag="ACHKCL_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5761681.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVSGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6477..7091
                     /gene="dmsD"
                     /locus_tag="ACHKCL_000640"
     CDS             6477..7091
                     /gene="dmsD"
                     /locus_tag="ACHKCL_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5761682.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7249..8541
                     /gene="clcB"
                     /locus_tag="ACHKCL_000641"
     CDS             7249..8541
                     /gene="clcB"
                     /locus_tag="ACHKCL_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5761683.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8494..9189)
                     /gene="bioD"
                     /locus_tag="ACHKCL_000642"
     CDS             complement(8494..9189)
                     /gene="bioD"
                     /locus_tag="ACHKCL_000642"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5761684.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9314..10534)
                     /gene="mlc"
                     /locus_tag="ACHKCL_000643"
                     /gene_synonym="dgsA"
     CDS             complement(9314..10534)
                     /gene="mlc"
                     /locus_tag="ACHKCL_000643"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5761685.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10669..11562)
                     /gene="ynfL"
                     /locus_tag="ACHKCL_000644"
     CDS             complement(10669..11562)
                     /gene="ynfL"
                     /locus_tag="ACHKCL_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5761686.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11669..12922
                     /gene="ynfM"
                     /locus_tag="ACHKCL_000645"
     CDS             11669..12922
                     /gene="ynfM"
                     /locus_tag="ACHKCL_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761687.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(12971..13090)
                     /locus_tag="ACHKCL_000646"
     CDS             complement(12971..13090)
                     /locus_tag="ACHKCL_000646"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5761688.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13346..13654
                     /gene="asr"
                     /locus_tag="ACHKCL_000647"
     CDS             13346..13654
                     /gene="asr"
                     /locus_tag="ACHKCL_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5761689.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            13747..13830
                     /locus_tag="ACHKCL_000648"
     CDS             13747..13830
                     /locus_tag="ACHKCL_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761690.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13930..14751
                     /gene="ydgD"
                     /locus_tag="ACHKCL_000649"
     CDS             13930..14751
                     /gene="ydgD"
                     /locus_tag="ACHKCL_000649"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5761691.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14790..15119)
                     /gene="mdtI"
                     /locus_tag="ACHKCL_000650"
     CDS             complement(14790..15119)
                     /gene="mdtI"
                     /locus_tag="ACHKCL_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5761692.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15106..15471)
                     /gene="mdtJ"
                     /locus_tag="ACHKCL_000651"
     CDS             complement(15106..15471)
                     /gene="mdtJ"
                     /locus_tag="ACHKCL_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5761693.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15883..16917
                     /gene="tqsA"
                     /locus_tag="ACHKCL_000652"
     CDS             15883..16917
                     /gene="tqsA"
                     /locus_tag="ACHKCL_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5761694.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16942..18330)
                     /gene="pntB"
                     /locus_tag="ACHKCL_000653"
     CDS             complement(16942..18330)
                     /gene="pntB"
                     /locus_tag="ACHKCL_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5761695.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18341..19873)
                     /gene="pntA"
                     /locus_tag="ACHKCL_000654"
     CDS             complement(18341..19873)
                     /gene="pntA"
                     /locus_tag="ACHKCL_000654"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5761696.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20397..21341
                     /gene="ydgH"
                     /locus_tag="ACHKCL_000655"
     CDS             20397..21341
                     /gene="ydgH"
                     /locus_tag="ACHKCL_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5761697.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21527..22909
                     /gene="ydgI"
                     /locus_tag="ACHKCL_000656"
     CDS             21527..22909
                     /gene="ydgI"
                     /locus_tag="ACHKCL_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5761698.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22946..23668
                     /gene="folM"
                     /locus_tag="ACHKCL_000657"
     CDS             22946..23668
                     /gene="folM"
                     /locus_tag="ACHKCL_000657"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5761699.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23665..24000)
                     /gene="ydgC"
                     /locus_tag="ACHKCL_000658"
     CDS             complement(23665..24000)
                     /gene="ydgC"
                     /locus_tag="ACHKCL_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5761700.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24129..24848
                     /gene="rstA"
                     /locus_tag="ACHKCL_000659"
     CDS             24129..24848
                     /gene="rstA"
                     /locus_tag="ACHKCL_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5761701.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24852..26153
                     /gene="rstB"
                     /locus_tag="ACHKCL_000660"
     CDS             24852..26153
                     /gene="rstB"
                     /locus_tag="ACHKCL_000660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5761702.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26229..27158
                     /gene="tus"
                     /locus_tag="ACHKCL_000661"
     CDS             26229..27158
                     /gene="tus"
                     /locus_tag="ACHKCL_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5761703.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27155..28558)
                     /gene="fumC"
                     /locus_tag="ACHKCL_000662"
     CDS             complement(27155..28558)
                     /gene="fumC"
                     /locus_tag="ACHKCL_000662"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5761704.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(28701..30347)
                     /gene="fumB"
                     /locus_tag="ACHKCL_000663"
     CDS             complement(28701..30347)
                     /gene="fumB"
                     /locus_tag="ACHKCL_000663"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5761705.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30546..31721
                     /gene="manA"
                     /locus_tag="ACHKCL_000664"
     CDS             30546..31721
                     /gene="manA"
                     /locus_tag="ACHKCL_000664"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5761706.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            31822..33330
                     /gene="ydgA"
                     /locus_tag="ACHKCL_000665"
     CDS             31822..33330
                     /gene="ydgA"
                     /locus_tag="ACHKCL_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5761707.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(33375..34639)
                     /gene="uidC"
                     /locus_tag="ACHKCL_000666"
                     /pseudo
     CDS             complement(33375..34639)
                     /gene="uidC"
                     /locus_tag="ACHKCL_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(34678..36051)
                     /gene="uidB"
                     /locus_tag="ACHKCL_000667"
     CDS             complement(34678..36051)
                     /gene="uidB"
                     /locus_tag="ACHKCL_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5761708.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(36048..37861)
                     /gene="uidA"
                     /locus_tag="ACHKCL_000668"
                     /pseudo
     CDS             complement(36048..37861)
                     /gene="uidA"
                     /locus_tag="ACHKCL_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(38252..38842)
                     /gene="uidR"
                     /locus_tag="ACHKCL_000669"
     CDS             complement(38252..38842)
                     /gene="uidR"
                     /locus_tag="ACHKCL_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5761709.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(39071..39838)
                     /gene="hdhA"
                     /locus_tag="ACHKCL_000670"
     CDS             complement(39071..39838)
                     /gene="hdhA"
                     /locus_tag="ACHKCL_000670"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5761710.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(39950..40978)
                     /gene="malI"
                     /locus_tag="ACHKCL_000671"
     CDS             complement(39950..40978)
                     /gene="malI"
                     /locus_tag="ACHKCL_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5761711.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            41153..42745
                     /gene="malX"
                     /locus_tag="ACHKCL_000672"
     CDS             41153..42745
                     /gene="malX"
                     /locus_tag="ACHKCL_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5761712.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            42755..43927
                     /gene="malY"
                     /locus_tag="ACHKCL_000673"
     CDS             42755..43927
                     /gene="malY"
                     /locus_tag="ACHKCL_000673"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5761713.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            44031..45032
                     /gene="add"
                     /locus_tag="ACHKCL_000674"
     CDS             44031..45032
                     /gene="add"
                     /locus_tag="ACHKCL_000674"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5761714.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(45068..46108)
                     /gene="ydgJ"
                     /locus_tag="ACHKCL_000675"
     CDS             complement(45068..46108)
                     /gene="ydgJ"
                     /locus_tag="ACHKCL_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5761715.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            46351..46476
                     /gene="blr"
                     /locus_tag="ACHKCL_000676"
     CDS             46351..46476
                     /gene="blr"
                     /locus_tag="ACHKCL_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5761716.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTMVQHK"
BASE COUNT        11440 a        12081 c        11472 g        11688 t
ORIGIN      
        1 ataaactttt atataacgat aaagaacagg gagtgagtta tgtccaaaaa tgaacgaatg
       61 gtgggcatca gccgcagaac actcgttaaa tctaccgcga taggttctct ggcgctggct
      121 gcaggcggtt tttctttgcc gttcaccctg cgcagtgcag cggcagcagt acaacaggcc
      181 agcgaacaag tggtctgggg tgcctgttcc gtcaactgtg gtagccgctg tgcacttcgt
      241 ctacatgtta aagataatga agtggcctgg gtggaaactg acaacaccgg cagcgatgag
      301 tatggcaacc atcaggtacg cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat
      361 catcccgatc gcttgaatta cccaatgaaa cgcgtgggta aacgcggtga aggcaaattc
      421 gaacggatta gctgggatga agccctggat actatcgcca gtagcctgaa gaaaaccgtc
      481 gaacaatatg gcaatgaggc tgtatatatt cagtactctt cggggatcgt tggcggcaat
      541 ataacccgct cttcgccatc agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt
      601 tcactcaacc agtatggctc ctacagcact gcgcaaattt cctgtgccat gccctacacc
      661 tacggcagta atgatggcaa cagcaccacg gatattgaaa acagcaagct ggtcgtgatg
      721 tttggtaaca acccggcaga aacccgcatg agcggcggtg gcatcactta tcttcttgaa
      781 aaatcccgcg aaaaatcgaa cgcaaaaatg attgttatcg atccgcgata taccgatacg
      841 gctgctggtc gtgaagatga atggcttcct attcgcccgg gcaccgatgc cgcgctggtt
      901 gcgggtattg cctgggtatt gattaacgaa aatctcgttg atcaaccttt tctcgataaa
      961 tactgcgtcg gctatgacga aaaaacctta cccgcagatg cacccaaaaa tggtcactat
     1021 aaagcctata ttcttggtga aggtgacgat aaaacagcga aaacgccgca gtgggcttcg
     1081 caaattaccg gtatcccggt ggaccgtatc atcaaactgg cgcgtgaaat tggcacagca
     1141 aaacccgctt atatctgcca gggctggggg ccgcaacgcc aggcaaacgg cgagctgacc
     1201 gcacgcgcta ttgctatgtt gcctattctg acgggcaatg tcggcatcag cggcggaaat
     1261 agtggcgcgc gtgaatcgac ctataccatt accatagagc gcctgccggt gctggataat
     1321 ccggtcaaaa cgtcaatttc ctgcttcagc tggacagatg ctatcgatca tggcccacaa
     1381 atgacggcaa tccgcgacgg tgtccggggc aaagataaac tggatgtgcc cattaagttc
     1441 atctggaact acgcgggaaa taccctggtt aatcagcatt ctgacatcaa caaaacgcat
     1501 gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc
     1561 tcatcggcaa aatatgccga cattctgctg ccagacctga tgaccgttga gcaggaagat
     1621 attattccta acgactacgc cggtaacatg ggatatctca ttttcctcca gcctgtcacc
     1681 agcgaaaaat tcgaacgcaa accgatttac tggatcctga gtgaagtcgc gaaacgtctt
     1741 ggacctgacg tctatcaaaa gttcactgaa ggtcgcacgc aggaacaatg gttacaacat
     1801 ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt cttacgacga actgaaaaaa
     1861 atgggtatct ataagcgtaa agatcccaat ggtcattttg tcgcctacaa agcatttcgt
     1921 gacgaccccg aggcaaatcc acttaaaaca ccttccggta agattgaaat ttattccagc
     1981 aagctggcgg aaattgcccg tacctgggaa ctggaaaaag atgaagtgat aagcccactg
     2041 ccagtttatg cctcaacctt tgaaggctgg gactcccctg aacgtagtac ctttccactg
     2101 caactgtttg gtttccatta caaatcccgt actcactcga cctacggcaa cattgatgtc
     2161 ctgaaggctg cctgccgtca ggaggtgtgg atcaacccta tagacgcaca gaaacgtggg
     2221 attgccaacg gcgatatggt gcgggtgtat aaccatcgtg gcgaagttcg gctaccagcc
     2281 aaagtgacac cacgcattct ccctggggtt agcgctatgg gccagggagc ctggcacgag
     2341 gccaatatgt ctggcgataa aatcgaccat ggcggatgtg tgaatacgct aaccactctg
     2401 cgcccttcac cactggcgaa gggaaacccg cagcacacga atctggtcga gatcgaaaaa
     2461 atataaccca ctacaaccat aaattctggc atgacatttt gtttgaaaag caataagtga
     2521 gtaatgatga aaatccatac cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt
     2581 ctgatgaaaa cctccgcact tggcagtctt gcgctggcaa gcagtgcttt cactctgcca
     2641 ttttcccaaa tggtccgagc ggcagaggca ccggtagaag agaaagcggt ctggagttcc
     2701 tgcaccgtta actgcgggag ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg
     2761 tactgggtgg agtctgatac gacaggtgac gacgtctacg gtaatcatca ggttcgtgcg
     2821 tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc ctgataggtt gaaatatccc
     2881 atgaagcgcg tcggcaagcg cggtgaaggt aaatttgaac ggataagttg ggacgaagcc
     2941 ctggatacca tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta
     3001 catgtcctgt acggaacagg cgtagatggc ggaaacatca ccaactcaaa cgtcccgtac
     3061 cgtctgatga actcttgcgg tggttttctc agtcgctatg gcagctacag taccgcacag
     3121 atcagtgccg caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc
     3181 gccaatacga aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggt
     3241 ggtggtgtca cttactacgt cgagcaagcc cgcgaacgtt caaacgcacg catgatcgtc
     3301 atcgatccac gttataacga cacagctgcc gggcgtgaag atgaatggct gcccattcgc
     3361 cctggcaccg atggcgcact ggcctgtgcg attgcctggg tactgattag tgaaaacatg
     3421 gtcgatcagc catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc
     3481 aacgcaccac gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata
     3541 gctaaaacgc cggaatgggc agcaaaaatc accagcatcc cggcagaaaa aattatccag
     3601 ttggcacgag agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa
     3661 cgacattcca acggcgagca aacatcccgc gctattgcca tgctttccgt tctcaccggt
     3721 aacgtcggca taaacggcgg caactcaggt gtacgcgaag gtagctggga tctgggggta
     3781 gaatggttcc cgatgctcga gaatcctgtt aaaacgcaga tttccgtctt tacatggaca
     3841 gatgccatcg accatggtac ggaaatgacc gcgacccgtg atggtgttcg tggaaaagaa
     3901 aaactggatg tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa
     3961 catggcgaca tcaatcacac caatgaggtg cttcaggatg acagcaagtg cgagatgatt
     4021 gttggcattg accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcccgac
     4081 ctgatgccaa cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat
     4141 gtgatcctcg cccaacccgc aacctcagca aaatttgaac gcaaacccat ctactggatg
     4201 ctgagtgaag tcgccaaacg cttaggaccg gacgtttatc aaacctttac tgaaggtcgc
     4261 agtcagcatg aatggatcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg
     4321 cccgactacg aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac
     4381 tacgtcgctt tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg
     4441 gggaaaatcg aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa
     4501 aaagatgaaa ttattcatcc ccttcctgcg tataccccag gttttgatgg ctgggacgat
     4561 cccctgcgga aaacctatcc actgcagtta acgggcttcc actacaaagc gcgtacccac
     4621 tccagctacg gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac
     4681 cccattgatg ctcaggctcg cggtatccgt catggcgata ccgtgcgggt atttaacaat
     4741 aatggagaaa tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc
     4801 atcgggcaag gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc
     4861 agtatcaata ttctgacctc tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac
     4921 agcaatcttg tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt
     4981 ttttatcgat tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt
     5041 taaagatctt ggcccggaag tcagtttccg ccgtatttat gaatatgctg gcggcgactg
     5101 gcaggaagat aatggcgtct ggcaccagaa cgtgttcgct tattatctct ccatctcctg
     5161 taaccattgc gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg
     5221 tgaagatggc tttgtggtgg ttgatgaaga tgtttgtatc ggctgccgct actgccacat
     5281 ggcttgtccg tacggcgcgc cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga
     5341 tggttgttat tcgcgcgtcg ccgaggggaa acaacccata tgtgtcgaat cctgcccgct
     5401 gcgtgcactt gagttcggtc caattgaaga gctgcgccag aagcacggca ctcttgctgc
     5461 cgtcgcgcca ctgccgcgcg cgcatttcac aaaacccaat atcgttatca aacctaacgc
     5521 caacagccgc ccgaccggcg ataccactgg ttatctggct aatccggagg aagtgtaaaa
     5581 tgggaaatgg atggcatgaa tggccactgg ttatctttac cgtattgggt caatgcgtag
     5641 taggcgcgct gattgttagc ggaattggct ggttcgccgc caaaactgac gcagaccgtc
     5701 agcacattgt tcgtggcatg tttttcctct ggctgttgat gggcatcggg tttattgcct
     5761 ctgtcatgca cctgggatcg ccactgcggg cgtttaactc gctgaatcga atcggagctt
     5821 ctggtctgag taacgagatt gctgccggtt ccatattttt tgccgttagc ggactatggt
     5881 ggctggtggc ggtcatcggt aaaatgccgc aagcgttggg gaaactctgg ttacttgtca
     5941 gcatggcgct cggcgtcatt ttcgtctgga tgatgacctg cgtgtatcaa atcgacactg
     6001 tgccaacctg gcataacggt tacactacgc tggctttctt cgtgacggta ttgttgagtg
     6061 gtccaatact tgctgccgca attctgcggg cagcccgcgt tacatttaat accacaccat
     6121 ttgccatcat tagcgttctg gcgctgatcg cttgcgccgg ggtgattgtg ctgcaaggtt
     6181 tgtctctgtc gtctattcac tcatccgtgc aacaagccag cgcactggta ccagattacg
     6241 cctccttaca ggtctggcgt gtggtattgt tatgcgcagg tctaggatgc tggctttgtc
     6301 cgctcatacg tcgccgtgaa cctcacgttg ccggacttat tctggggtta atcctcattc
     6361 ttggcggtga gatgatcggg cgtgtgctct tttatggcct gcacatgacg gtaggaatgg
     6421 ctattgcagg ttaacaaatt ggtgcggggc aacccgcaca tttcaggatg ttaggaatga
     6481 cccatttttc acagcaagat aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt
     6541 attacgctcc agagagtgct gaagccgcac ctctggttgc ggtactcacc agtgacggct
     6601 gggaaactca gtggcctcta ccagaagcgt cattagcgcc gctggtgacg gcttttcaga
     6661 cacagtgtga agagactcac gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac
     6721 tgccgtctcc gccgtggggg tcggtctggc tggatcgcga atctgtgctg tttggcgatt
     6781 caacattggc acttcgtcag tggatgcgcg agaaaggcat tcagtttgaa atgaagcaaa
     6841 acgaaccgga agatcatttt ggttcactgc tgttgatggc tgcgtggttg gcggagaatg
     6901 gtcgccagac agaatgcgaa gaattgctgg catggcacct ttttccctgg tcaacacgtt
     6961 ttcttgacgt ttttatcgaa aaagcagaac accctttcta ccgtgcactg ggtgaactgg
     7021 ctcgcttaac gctggcgcaa tggcaatcac aactgttaat tcctgtcgcg gttaaaccac
     7081 tctttcgata ggatcaccgt aatattgccg gatggtgacg attacgcgcg attcggcaac
     7141 aatacgttac agtgtcacgt tttttgtctc ttaaagcacg cactgctttt gcagctggcc
     7201 tcttttgccg caaaatagtc gcccgtgttt cattgcccat ttctgctcat gcatcatcta
     7261 cacacctatc cggatctgcg cactatgttc cgccgtctgc ttatcgcaac agtcgtcggt
     7321 attctcgcgg cctttgccgt tgccgggttt cgtcatgcga tgctgctact ggagtggttg
     7381 ttcctcaata atgactccgg cagtctggtc aatgcagcga caaacctttc cccctggcga
     7441 cggctgctaa ctccggcgct cggcggactg gcggcgggtt tgttgttgat gggctggcag
     7501 aaatttaccc agcaacgccc tcatgcgccg accgattaca tggaagcgtt gcaaaccgat
     7561 ggtcagtttg attacgcagc aagcctggtt aaatcgcttg cctctctgct ggtagtaacc
     7621 agcggcagtg caattggtcg cgaaggtgcg atgattcttt tagctgccct tgccgcctcc
     7681 tgttttgccc aacgttttac gccacgccag gagtggaaat tatggatcgc ctgtggggcc
     7741 gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg gcagtttatt tatagccgaa
     7801 gtgctgtttg gcactatgat gttggcctct ctcggcccgg tgattatttc cgccgtcgtg
     7861 gcattgctgg ttagcaatct gattaatcat agcgacgcgt tactctacag cgtacaactc
     7921 tcagtgacgg ttcaggctcg tgactatgcg ctgattatca gtacaggtgt gctggcaggt
     7981 ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc atcgtggatt tgtgagtctc
     8041 aaacttgcgc cgccctggca actggcacta ggcgggttga tcgtgggtct gctttccctg
     8101 ttcacacctg cagtgtgggg caacggctat agcaccgtac aatccttttt aaccgccccg
     8161 ccactgttaa tgatcattgc cgggatcttc ctttgtaaac tgtgtgccgt gctggcgagt
     8221 agcggttccg gcgcacccgg tggtgtcttt acaccgacgc tatttatcgg tcttgccatt
     8281 ggcatgttgt atggtcgtag tctgggatta tggttccctg atggcgaaga aattacgctt
     8341 ttactcggat tgaccgggat ggcgacactg ttggcggcaa ccacgcacgc gccgattatg
     8401 tcgacgttga tgatatgtga aatgaccggg gagtatcagc tactccccgg tttattgatt
     8461 gcctgcgtaa ttgcgtcggt aatctcgcgg acgttacacc gtgactctat ctaccgccag
     8521 cacactgcgc agcatagcta agcggatgta ttgccccagt tcacgctgtt cggcgcgcgg
     8581 cagataaggc agttcaccaa tgagcggtgc cggaagtttt ttaccgagca catcaatgat
     8641 ttccgcataa tgcgccagtc ctgggttgat tcggttagcc acccagccaa tgagcggcag
     8701 cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg ttaatgcaac cttcctgaat
     8761 accgacaacc atcaacaccg gcagttgttc ctgcaccacc cattcagaga gtggacgcaa
     8821 atcattcatc agactacgcc agccgccagt cccttctacc acgacatgat cgactttttc
     8881 ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg attgggcaac tgtgcgccac
     8941 gctactttct tcttcgctta acgcgatagg attaactgct tcataaggca gttcgatggt
     9001 tgaaacactc tgcaacacca gggcatcttt attacgcagc ccttcgggtg tctctttgct
     9061 ccccttcgct acgggtttat atcccgcaac cgtttttccc tgggaggcta acgcttgtag
     9121 caatgcgcgg gaaaccaccg ttttccctac agaagtgtct gtaccggtaa taaagaaacg
     9181 cttcagcatc actaactcca ccgttatgct tcacaaatat aaaccaggaa aataattaac
     9241 cttgaaagtc taagttatgc tttcctggcc caaattgaga tagcgcaaat tttggtagaa
     9301 cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa agaaccgtta tacatcgcgt
     9361 cttttaccag cgcagcccct gccatcgtgc cctggttaga aaactgagta ctctcaacgc
     9421 taatgtgctg actatacgca ggaagggcct gctgacggat gctatctgag atgaccggga
     9481 agaggatatc tgccgcttta cttaacggtg agccaatcag tattttttgt gggttaaata
     9541 aattcaccat gatggcaaga atgcgcccta catgcgcgcc caccccggta atgatgtctt
     9601 ttgccagcag atcgccgcgc aatgccgcct gacacaatga gtccacggtt aacggttgcc
     9661 catgtaacat cgagctcatg gattgattga gacgcagctg tgccagctca agaatactgt
     9721 ccacactggc gatggtttcg aggcagccgt ggttcccgca ataacagcgt ttaccatatg
     9781 ggtctacctg tgtgtgacct atttccacga ggctactgct gcctgcgtgt agcagatgac
     9841 catcggtaat gacgcccgcc cccacgttgt gatcgataac cacctgaatc acatcgcgcg
     9901 ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca tgcgctgata tcatgctgaa
     9961 tataaaccgg aacgccggta tgctgctcca gagcctcgcc gagcggcatc tcttttacat
    10021 cctcgtagaa cggcatgcga tgtacaatgc cattttccgt atcaataatt cccggcaggg
    10081 ttatggcaat cgaagttaga cgctcaagtt ttttctggtg gcggataaaa aactgatcga
    10141 tatgggaaat gatacgatcc agcaatggcg agtcatcttt taacgccagt tcctgcgact
    10201 cttccaccac cagtttgctg ctcagatcgc gcagagcaag gaaaatctcc ccgcgactaa
    10261 tgcgcagaga aagatagtgc caggcttcag tttcaaccac cagccccacc gccggacggc
    10321 cacggttccc cgcttctttg atttccagct cttgcaccag gtgtgcttcg agcatctcac
    10381 ggacaatttt agtgatactg gcaggagcca gctgcgccag acgggaaaga tcgatacgcg
    10441 agactggacc aagctgatca atcaggcgat aaaccgcgcc cgcgttggtc tgctttattt
    10501 gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata ctccctatat tttcgcgctc
    10561 cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt gtcgtcaaat ttttacttag
    10621 gcatgtgatt aacagcacat ttttcgggct ttttcgctga aatttccctc acctgagagc
    10681 attcagcaga tgaatacgaa agttacgcgc agctggactt tgttcatgat gtttcggcca
    10741 gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc ggcacccagc gcatttcgtt
    10801 gagctgaaca cgtttaaatg acgcaggcaa aattgaaaca cccagaccgg cggaaaccag
    10861 accgatgatg gtcattgcct cgcccacctc ctgagtgatg acgggcgtca aatggtaacg
    10921 tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg acgtgcggat caaaaaagac
    10981 aaagggttct ttcgccagtt cagccagcgt tacattcggg ttatttgcca ggggatgatc
    11041 gtgcgggatc atcgccataa gcggttcatg gacgatgact gcgtgttcaa gcgtctccgg
    11101 taacgctgtg ttacgcagca atcccatatc cagcgttcct tcaatgagcg gagcgatttg
    11161 ctcgcgagtg ttcatttcgc gggtttgtaa atggacatcg ggataatctc ggcgaaacag
    11221 cgataacgta tcggacaccg cccgaataaa aggagccgac gaagtaaaac caatgcgcaa
    11281 ctcccccgct tcaccctgat gcagtctttc agcgcgagcg gcagcgtcat ccaccataga
    11341 caggatttgc cgactatctg caagaaactg ttttcctgct gccgtcagca atacactgcg
    11401 attggttcgt gccagcagtc gggcaccaat ttgttgctcc agcgcctgaa tctgctgact
    11461 tagcggcggt tgcgaaatat tcaggcgggc agcggcgcgc ccgaaatgca gctcttccgc
    11521 aacagcaaca aagtaacgca gatgacgaag ttcaatattc atatttaaaa catcttattt
    11581 gagattatta atatattaga cagaacaatt cgattttcct aacctatgta taagcctgat
    11641 ccacaggcat atttagcaag gatttcaagt gagccgtact acaactgttg atggcgctcc
    11701 ggcaagcgac actgacaagc aaagcatttc tcagccaaat caatttatta aacgcggtac
    11761 gccgcaattt atgcgcgtca ccctggcgct gttctctgcc ggactggcaa catttgcact
    11821 tctctattgt gtgcaaccta tccttccggt gctttcgcag gagtttggct taacgcccgc
    11881 gaacagtagt atttcactgt ccatttccac ggcgatgttg gctattggtt tgctgtttac
    11941 tggcccccta tccgatgcca ttggtcgcaa accagtgatg gtaacggcgc tactattggc
    12001 ctccatttgt acgttacttt cgacaatgat gaccagctgg cacggcattt tgattatgcg
    12061 cgccttgatt gggctttcgt taagtggcgt ggcagctgtt ggcatgactt atcttagcga
    12121 ggaaatccac cccagtttcg tggccttttc gatggggttg tatatcagcg gcaactcaat
    12181 tggcggcatg agcggacgct taattagtgg tgtcttcacg gactttttca actggcgaat
    12241 tgctctggcg gctatcggtt gtttcgcgct agcctcggcg ttgatgttct ggaaaatcct
    12301 cccggaatca cgccattttc gcccgacttc gctgcgacct aagtcgttgt ttatcaactt
    12361 tcgtctgcac tggcgtgacc ggggattacc gttattgttc gcagaaggct ttttgctgat
    12421 ggggtcgttc gtcacgctgt ttaattacat cggctatcgg ttgatgctct ccccctggca
    12481 tgtcagtcag gccgtggttg gcttattatc gctggcttat ttgaccggta catggagctc
    12541 acccaaagcc ggaaccatga ccacccgcta tgggcgtggt ccagtgatgt tgttttcgac
    12601 gggggttatg ctgtttggtt tactgatgac cttattcagc tcgctgtggc tgatctttgc
    12661 cggaatgtta ctcttctcag caggattctt cgcagcccac tcagttgcca gcagctggat
    12721 cggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg ctgtatctgt tcagttacta
    12781 tctggggtcg agtattgccg ggacgctggg tggtgttttc tggcataact atggctggaa
    12841 cggcgtcggc gcatttattg ctctgatgct ggtcattgct ctgctggtcg ggacgcgttt
    12901 gcatcgtcgt ctgcacgcct gaaaaataag tccggactgc ggtaaatacc cgtccggact
    12961 tattgcctgc tcaaaccaac gttaatagcc atcctaaaat agacgaagcg ccagccaatt
    13021 cccgcagcgc gtctagcgtc atcaggatta taagtaccca aataaacgga ttcattttgc
    13081 tgtgtgtcat ttattactga tgcgcagtta ttctaccgct ttgtaagtag taaaatagtt
    13141 aacccgatca agactactat tattggtagc taaattttcc ttaagtcaca atacgttatt
    13201 atcaacgctg taatttattc agcgtttgta catatcgtta cacgctgaaa ccaaccactc
    13261 acggaagtct gccattccca gggatatagt tctttcaacg gccccgcagt ggggttaaat
    13321 gaaaaaacaa attgagggta tgacaatgaa aaaagtatta gctctggttg ttgccgctgc
    13381 tatgggtctg tcttctgccg cctttgctgc agagactgcg accacacctg ctccgactgc
    13441 gacgaccacc aaagcagcgc cggcgaaaac tacacatcat aaaaaacagc ataaagcagc
    13501 acctgcccag aaagcgcagg cggctaaaaa gcatcataaa aatacgaaag ctgaacagaa
    13561 agccccagaa caaaaagcgc aggcagcgaa gaaacacgcc aagaaacaca gccatcagca
    13621 accggcaaaa cctgctgcac aacccgcagc gtaagttttc aacagtaatg ctggcgcgcc
    13681 ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt tagcaccgcc tttagcggag
    13741 ggtagtatgg tgggccgtta tcgctttgag ttcattctta tcatccttat tttatgcgca
    13801 ctgattgccg cccgttttta tctttcctga tcgtagttat ctgattttac tcccactttc
    13861 atcccgtccc gtctatagta tttatgaggg tttgctttta ataatcataa ttacccacca
    13921 gagtgtgata tgcgtacaac cattgctgta gtgttgggtg caattagttt gacgtctgct
    13981 tttgtgtttg cagataaacc agacgttgcc aaatcggcaa acgatgaggt cagcaccctg
    14041 ttttttggtc atgatgatcg tgtgccagtg aatgacacga cccaatcacc gtgggatgcg
    14101 gttgggcaac tggaaacggc cagcggcaat ttatgtacgg cgacgctgat tgcacccaat
    14161 ctggcattaa cggcaggaca ctgtttattg acgccaccaa agggtaaagc cgataaagca
    14221 gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct atgacatcca cgacatagaa
    14281 ggccgcgttg atccgacact gggaaagcgg ttaaaagcag atggggatgg ttggattgta
    14341 cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc tacgtaatcc cccttctggc
    14401 attacgccgt tgccgttatt tgagggagat aaagccgcgc ttaccgccgc attaaaagcg
    14461 gcaggtcgta aagtgactca ggcaggatac cctgaagatc atctcgatac gttgtacagt
    14521 catcaaaact gtgaagtgac tggctgggcg caaacgtcgg tgatgtcaca tcagtgcgat
    14581 accttgccgg gtgacagcgg ttcgcctctg atgttgcata ccgatgacgg ctggcaatta
    14641 attggggtgc aaagttcggc tcctgccgcg aaagatcgct ggcgcgccga taaccgggcc
    14701 atttctgtta ccggttttcg cgacaagctg gatcaactgt cgcaaaaata atgttcaaac
    14761 gctgcccgac agcgcgggca gcgtcttcat caggcaagtt tcaccatgat cattccagcc
    14821 aacagcaaga ccaggccaat ccagccttta cgatttaacc gttgaccaaa caagatccaa
    14881 cctgcggcta acgtggcggc aataccaaac ccgccccaca atgcataagc gacagacaag
    14941 tcgatccctt taacggcctg agaaagcgca ctaaaggcag ccagcaccgc cgccagggag
    15001 agcaagccaa atattttgcg acgaaagccg tcagaaaatt tcaaaaagac gttagcaacg
    15061 atttccagca cgattgccaa tgccagccag gcggcgtgaa cccattcaaa ctgcgccatg
    15121 gttcacctcc agttcaggtt tacgtgcttt acgggtacct gatttgatca acactatccc
    15181 ggcgaccagg gtggttaacc cggcaatttt catcagcgat aaactttcgt cgaataacaa
    15241 aacgctaaac aaggtaataa ataaaatacc gataccttcc cacagcgcat aagccacacc
    15301 taaggctatt tttttaacag cgaaagagag aaatatatac gacagagaaa tcatcaccag
    15361 cattaaaata aagccgccat ttccctcact gacgctcgcc catttcatag acagcgtacc
    15421 ggtaatttct gtagcaatag ccagacctaa taaaatccaa taaatataca ttgtccttct
    15481 cctgcaagag aattatttta attttcgctt aattcagcga aaccaaagta aagtggtcaa
    15541 gccccgcgta tagggcaagc ttaggcagaa gaaaggacta aagcgcgttg cgccagtgct
    15601 gctcacctac gagcaagata gtggatgagg tacgaagatg ggtaaatgta gaaaataacg
    15661 tcctgaacaa attgtccata atattacaat tatccgcagt gttgcttctc gtcatcgcgg
    15721 atgataattg tcctcggtag ttgaacacgc ctgatttgta tcatagctta agaattaact
    15781 caaaatattt tcacttcttt acctgagcgg tttgattttc gttatgatga cggagcgaaa
    15841 aagacattat tattagcaaa ggaagaaaaa acggggacaa gcatggcaaa gccgctcatc
    15901 acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc tggtcattat tctctgcggt
    15961 atccgttttg ccgccgagat catcgtgccg tttattctcg cattatttat tgctgttatt
    16021 cttaacccgc tggtgcaaca catggtccgc tggcgtgtgc cgcgtgtact ggcagtgtcg
    16081 attttgatga ccatcatcgt gatggctatg gtgttgctgt tagcttatct gggttccacg
    16141 ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact ctattatgac gccgctgcaa
    16201 gctcttgagc cgttgttgca acgcgtaggg attgacgtct cagttgacca actggcgcat
    16261 tacattgatc cgaacgcggc gatgacgttg ctcaccaact tattgacgca gttatctaat
    16321 gccatgtcat caatattttt attgctgctg acggtgctgt ttatgctgct cgaagtgcca
    16381 caattgcccg gaaaatttaa gcaaatgatg gcgcgtccgg ttgaagggat ggcggcgatt
    16441 cagcgtgcga ttgacagtgt gtctcattat ctggtgctga aaacagccat cagcatcatc
    16501 accggcctgg tcgcatgggc gatgctcgcc gccctcgatg ttcgcttcgc ttttgtctgg
    16561 ggattgctgg cctttgcgct taattacatc cctaatattg gttcagttct cgcggcaatc
    16621 ccccctattg ctcaggtact ggtgtttaat ggcttctacg aagcgttgtt ggtgctggcg
    16681 ggatatctgc tgattaatct ggtcttcggc aatattctgg agccacgcat catggggcgt
    16741 gggctgggac tttccacatt ggtggtattt ttgtcgttga ttttttgggg atggttgtta
    16801 ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta ttgtcaaaat tgcgcttgaa
    16861 caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg atctcaataa agagtgacgg
    16921 cctccgcaga ggccgtcagg gttacagagc tttcaggatt gcatccacgc tggctttggc
    16981 gtcaccaaac agcatgtggg tgttttcctt gaagaacagc gggttttgca caccagcata
    17041 gccagtgttc atcgaacgtt taaagacaat cacgttctgc gctttccaca cttccagcac
    17101 aggcatacca gcaatcggac tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt
    17161 agcaccaatc accagtacgg tatcggtatc agcaaagtca tcgttgatct cgtccatttc
    17221 cagcacgatg tcatacggta cttttgcttc agccagcaat acgttcatat gtccaggcaa
    17281 acgccccgca accgggtgga taccgaaacg cacgttgata ccacgagcgc gcagtttctc
    17341 ggtaatttca gcgacaggat attgcgcctg cgcgactgcc atgccgtacc ccggagtaat
    17401 gatcactgaa tgggagtttt tcaacagttc cgctgtctct tctgcggtga tttcgcggtg
    17461 ctcacccact tcctgatcat cgccagtaga agagccgtcg gtgccgaaac cacccgcaat
    17521 aacgctgata aaggaacggt tcatcgcctt acacataatg taagaaagga tagcccccga
    17581 ggaaccgacc agcgcaccgg tcacaatcag caggtcgttg ctgagcataa agcccgcagc
    17641 cgcagccgcc cagccggagt acgagttcag catcgacacc accaccggca tatctgcacc
    17701 accgatggag gcgactaaat gccagccaaa taccagcgca atggcggtca ttattagcaa
    17761 tgccagcact tgcaggccga cgctatccgt gcgaacaaat acaatcagca gcaggaagga
    17821 aacgaccaga gctgccaggt tcattttgtg acggtttggc agcatcaacg gtttagacga
    17881 aattttgcca cacagtttgc cgaacgccac caccgaaccc gtgaacgtta ccgccccgat
    17941 gaagataccg agaaacactt ccgtcaggtg aatattgacc agaatcggtg ccattcccgc
    18001 gtcatgatgc agatagctgt taaagccaac cagcactgcc gccagaccca cgaagctatg
    18061 caggatcgcc accagttctg gcatttcggt catttcaact ttcttcgcca gacggatacc
    18121 aattgcccca ccaatgacca tcgccagcaa gatccagcca acgttacccg tatccggtcc
    18181 aaaaatggtg gcgattagcg caatcgccat cccggcgata ccgaagttgt taccctggcg
    18241 agacgtttca tgtttcgaaa gcccggccag actgaagata aacaggatcg cggcaacaat
    18301 gtatgcagct gtaactaatc ctccagacat atgttacccc ttaatttttg cggaacattt
    18361 tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt aatgctggct ataagcaccg
    18421 cgataaaact aaggaagcta acccagccgc cctggccaat ctgcaacagt gctccgacaa
    18481 caataatccc tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc gcgtgcgata
    18541 cgttccacac cacgtaataa ccgacaacgc aggccagcgc gaaaacggtg aagtgcccaa
    18601 gaaactcttt cggcgcaacg cttgccagcc agccgaaaag gatgattgcc agcgccatca
    18661 acgcgtattt acgccacggt gaacaggcac atttttcctc agttttcact tccggtgccg
    18721 ctttttgtgc cacctgcggc tgagctgata cctgaatcgg cggtgccggc caggtaattt
    18781 cgcccgcacg gatcacagtc acgccgcgaa tcaccacatc atcaaaatca acagtgatgt
    18841 taccgttttt ctctttgcac aacagtttca gcagattaac gaggttagtg ccgtaaagct
    18901 gtgaggattg cgtcggcaga cggcccggaa gatcggtata gccaatcact ttgacaccat
    18961 tttccgtagt gaagatttca cccggcacgg tgtattcaca gttgccgccg ttttgggctg
    19021 ccaggtcgac aatcacactg cccgccttca tggagtcaac catttcacgg gtaattagct
    19081 tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat gatatcgacc tcttttgcct
    19141 gggcggcaaa gagttccatt tccgctttga tgaacgcgtc cgacatcact ttggcatagc
    19201 catcgccgct gcccgcttcc tctttaaaat ccagctcgag gaattccgcg cccatacttt
    19261 gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg cacaatcgcg ccgagactgt
    19321 ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc aatcaccatc acttttgccg
    19381 gtggcacttt cccggccgca gtaatttgcc cggtaaagaa gcacccaaat tcatgtgccg
    19441 cttcaacaat ggcgcgataa ccggcgatat tcgccatcga gcttagtgcg tccagcgatt
    19501 gtgcgcgtga gatacgcggc acagagtcca tcgccatcac ggtcacgtta cgttccgcaa
    19561 gtttttgcat taattccgga ttctgcgcag gccagataaa actcaccagc gttgtcccag
    19621 gattcagtaa cgcaatttca tcatctaacg gcgcattgac cttcagaatg atctctgact
    19681 gccagacgct attcccttct acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa
    19741 aacttgccag ttgacccgcg ccgctctcta ccgcgacggt aaaccccagt ttcagcagct
    19801 gttccactgt ttttggcgtt gctgcaacac ggggttcatt ggttaaccgt tctcttggta
    19861 tgccaattcg catgatattc ccttccatcg gttttattga tgatggtttg cctgtgtcag
    19921 gagccacaca agccgctcat gtacgagcta aatgttactc cgttaaaata aattagtaac
    19981 aaacgcccta taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc
    20041 ggcgtgaact cgcaaaatat taacgattca gccgtgatag tgggataaac accttagaac
    20101 gccggataaa gactgataat tgtcttcgac ggtcgggtaa aacgagacat cgccccggca
    20161 cgaatcacta cttaacatta aattaactta tacaattcag ttgcttcagt agtaatgatg
    20221 ctgatacggc tgttttttaa gcatagacgg tcatttgagc aggattaaaa ttggcttaag
    20281 gaatgtgata tgaaaaatga cgcagacagt tacaccgttt aaatgcaata atcagccacg
    20341 tttctcgtta ataacaatac cagtacctgg tttgcgcaag gcgaaggatt atttttatga
    20401 agcttaagaa caccctcctg gcgtcggcac tgctttctgc tacggcattc tccgttaacg
    20461 cagcaacaga actgacaccg gagcaagcgg cagcggttaa accttttgac cgtgtagtgg
    20521 ttaccggtcg ttttaatgct attggcgaag cggtgaaagc cgtttctcgt cgcgcagata
    20581 aagaaggtgc cgcctctttt tatgttgtcg acacttctga ttttgggaac agcggtaact
    20641 ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa agcagaagaa acaagtaatc
    20701 gcgtaattaa cggtgttgtc gaactgccga aagatcaggc tgttctgatt gaaccgtttg
    20761 acacggtcac cgtccagggc ttctatcgta gccagccaga agtcaatgat gccatcacca
    20821 aagcggcaaa agcgaaaggt gcctactctt tctacatcgt tcgtcaaatc gatgccaacc
    20881 agggcggcaa ccagcgtatt actgcattca tctataaaaa agatgctaag aaacgtatcg
    20941 tccagagccc ggatgtgatc ccggcagatt ccgaagcagg acgtgcagct ctggctgccg
    21001 gtggcgaagc cgcgaagaaa gttgagatcc cgggtgttgc gactaccgca tcaccaagtt
    21061 ctgaagtcgg tcgcttcttt gaaacccagt catcaaaagg cgggcgttac actgtcacgc
    21121 ttccggatgg cactaaagtc gaagaactga acaaagcgac agcagcgatg atggtcccgt
    21181 tcgacagcat caaattctct ggcaactacg gcaacatgac cgaagtctcc tatcaggttg
    21241 cgaaacgagc cgcgaagaaa ggtgctaaat attaccacat cacccgccag tggcaggaac
    21301 gtggtaataa cctgaccgtc agtgcagatc tgtataaata actattcagt tactcttata
    21361 aggcggcttg atgccgcctt tcgcattttt agtcacaaat ttccccgcga ccattgcatg
    21421 cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa actccatttt tatgcgtttt
    21481 gccctaatat ttattcttct tatcacgttt taatcactgg atatcgatgg aaaagaaact
    21541 gggactgagc gcactcaccg cgctggtatt aagctcaatg ctgggcgcgg gtgttttcag
    21601 tctgccgcaa aatatggcgg cagttgccag cccggcagca ctgctcatcg gctggggtat
    21661 tactggcgct ggcattttat tgctggcctt tgccatgctg atcctcacgc gcattcgtcc
    21721 cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg tttggcgagc taatcggctt
    21781 ttgttccgca tggggatact ggctgtgcgc agtcatcgcc aacgtctcct atctggtgat
    21841 cgttttttcc gcgttaagct tttttacgga cacgccggaa ttgcgcctgt ttggcgatgg
    21901 taatacctgg caatcgatag tcggtgcatc ggctttattg tggattgttc actttttgat
    21961 cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg gcgacactgg caaaattgtt
    22021 gccgctgggt ctgtttgttg tgctggcgat gatgatgttc aaactggata tcttcaagct
    22081 cgacttcacc ggacttgcac ttggcgtacc tgtttgggaa caggtgaaaa acaccatgct
    22141 gatcaccctg tgggttttca ttggtgtgga aggtgccgtg gttgtttctg cgcgtgcgcg
    22201 taataaacgt gatgttggca aagcgacact gctggcggtt ctctccgctc tgggcgttta
    22261 cttgctggta acgctgcttt cgctgggagt ggtggcacgt cctgaactgg ctgaaattcg
    22321 taacccgtca atggccggtc tgatggtgga aatgatggga ccgtggggcg aaatcatcat
    22381 cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta agctggacca tcatggcggc
    22441 agaagttccg ttcctggctg ccactcataa agcattccca cgcatttttg cgcgccagaa
    22501 cgcacaagct gcgccatccg cctcactgtg gttgacaaac atctgcgtgc aaatttgtct
    22561 ggtactcatc tggctgaccg gttccgatta caacacgttg ctgaccatcg cctcagaaat
    22621 gattctggtg ccctatttcc tggtcggcgc gttcttgctg aaaatcgcaa cccgtccact
    22681 gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta tggttattgt acgcctctgg
    22741 tccaatgcac ttgctgttat ccgttgttct gtatgcaccg gggctgctgg ttttcctgta
    22801 tgcgcgcaaa acgcatacac atgataatgt actgaaccgt caggaaatgg tgctgattgg
    22861 catgctgtta attgcctcag ttccggcgac ctggatgctg gtgggataac gttcatccca
    22921 tcgttggata gcaaaggaga taacgatggg taaaacccag cccttgccaa tattaattac
    22981 tggcggaggt cgtcgcatcg gcctcgccct cgcatggcat ttcattaatc aaaagcaacc
    23041 ggtgattgtc agctatcgga cacactatcc agccattgat ggactgatta atgcaggtgc
    23101 gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg atggcgtttg ccgatgaagt
    23161 actaaaaagc acccatggtc tgcgtgctat tttgcataac gccagtgcgt ggatggcgga
    23221 aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg atgcagatcc acgttaatac
    23281 cccatacctg ctcaaccatg cgctggaaag attactgcgt gggcacggac acgccgccag
    23341 cgatatcatt cactttaccg attatgtggt ggagcgcggt agcgacaaac atattgcgta
    23401 tgctgcaagc aaagcggcac tggataatat gacccgctcg tttgcccgca agctggcacc
    23461 ggaagtgaaa gtgaattcta ttgcgccatc gctgatcctg tttaatgaac atgatgatgc
    23521 cgaatatcga caacaggcgc tgaataaatc attgatgaaa accgcgcctg gcgagaaaga
    23581 agtgatcgac ctggtcgatt acttacttac cagttgcttt gtcaccggac gcagtttccc
    23641 acttgatggc ggtcgtcatc tgcgttaatg cagctttatc cagcatataa tcaacaccca
    23701 tgcgcttatc ccccaacacg caacagaacc aacaaaagcg gcgggcaagc gcatcattcc
    23761 ggtgaaatac cacagcgaca ccaggtaaac aaaataggga ataatcgacc acatactaaa
    23821 aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg gcaacaatat aatgcgcgat
    23881 aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata taataatttt tcgtttttgc
    23941 taaaacacca atcaacagca ctaccagcgc accgagcgcg gctttgatta ccagccccat
    24001 ctttttacct taacacttcc ataacaagtc atcagtagaa tacctgatga aaacttgttt
    24061 agaaacgatt gatagtaagt aaaaacagcg cggtgtattg tgacgttttt atatctaccg
    24121 tgaatgttat gaacactatc gtatttgtgg aagatgatgc ggaagtcggt tcactgattg
    24181 ccgcgtacct ggcaaaacat gatatacagg ttaccgtaga gccgcgcggc gaccaggccg
    24241 aagaaaccat tttgcgagaa aatccggatt tggtgttact cgacatcatg ctaccaggca
    24301 aggacggcat gaccatttgt cgtgatttac gcgcaaagtg gtccggaccg attgttcttc
    24361 taacctctct cgatagcgat atgaaccaca tcctggcact ggaaatgggt gcctgcgact
    24421 atattctcaa aacgacgccc cctgctgttt tgttagcgcg tttacgtttg catttgcgtc
    24481 agaatgagca agccacgctg accaaaggtc ttcaggaaac gtcactgact ccctacaaag
    24541 ccctgcattt cggcacgttg accatcgatc ctatcaaccg cgtagtcacc ctggctaaca
    24601 ctgaaatctc gctctcgaca gctgatttcg aattattgtg ggaattagct acccatgccg
    24661 ggcaaatcat ggaccgcgat gcattgctga aaaatttacg cggcgtcagt tatgacggcc
    24721 tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa aaaactgctc gataacgccg
    24781 ctgaacctta tcgcattaaa actgtgcgta acaaaggcta tctttttgcg cctcatgcat
    24841 gggaataagc gatgaaaaaa ctgtttatcc agttttacct gttattgttt gtctgcttcc
    24901 ttgtgatgtc tctgctggtt gggttggtgt acaaatttac cgccgaacgc gcgggcaaac
    24961 agtcgctgga tgatttgatg aacagttcgc tgtatctgat gcgcagcgaa ttgcgtgaga
    25021 tccccccaca tgactggggt aagacgctga aagagatgga tttaaatctc tctttcgatc
    25081 tgcgagtcga gccactgagt aaataccatc ttgatgatat ttccatgcac cggctgcgtg
    25141 gcggcgaaat tgtcgccctg gacgatcagt acactttttt gcagcgtatc ccgcgcagcc
    25201 actacgtgct ggcagttggt cctgttcctt atctttatta tctccatcag atgcgattgc
    25261 tggatatcgc cctgatcgct tttattgcta tttccctcgc ctttccggtg tttatctgga
    25321 tgcgtccgca ctggcaggat atgttaaaac tggaagcagc ggcgcaacga tttggcgatg
    25381 ggcatctcaa tgaacgtatc cactttgatg agggttcgag ctttgaacga cttggtatcg
    25441 catttaacca gatggcggac aatatcaacg ccttgattgc cagcaaaaaa cagcttattg
    25501 acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga ctggagatga
    25561 gcgataacct gagcgccgcc gaatcccagg cgttgaatcg tgatatcagt caacttgaag
    25621 ctttaattga agagctgctg acttatgccc gactcgatcg cccacaaaac gagcttcatc
    25681 ttagcgaacc agacctgccg ttgtggctgt caacgcatct ggcagatatt caggcagtaa
    25741 cgcccgataa aacggtacgg ataaaaacgc tcatgcaagg ccattatgcg gcgttggata
    25801 tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa tgccctgcgc tactgccatt
    25861 caacggttga aaccagcctg ctactgtcgg ggaatagagc gacattaatt gttgaggatg
    25921 atggcccagg gattgcccca gaaaaccgcg aacatatctt tgaacctttt gttcgcctcg
    25981 atcccagccg ggatcgctca accggcggct gcgggctggg gctggcaatt gtccactcta
    26041 tagcactggc aatgggcggt acggttaatt gtgacaccag cgaactgggt ggtgcccgct
    26101 tctcgtttag ctggccgtta tggcataaca tcccgcaatt tacctctgct tgacactacg
    26161 cgcacggtgg tcaagtcacc accactgtgc tataaattaa ggatgttgta actaaagtgg
    26221 ttaatattat ggcgcgttac gatctcgtag accgactcaa cactactttt cgccagatgg
    26281 aacaagagct ggctgcattt gccgctcatc ttgagcaaca caagctattg gttgcccgcg
    26341 tgttctcttt gccggaggta aaaaaagagg atgagcataa tccgcttaat cgtattgagg
    26401 tgaaacaaca tctcggcaac gacgcgcagt cgcaggcgtt gcgtcatttc cgtcatttat
    26461 ttattcaaca acagtccgaa aatcgcagca gcaaagcagc tgtccgtctg cctggcgtgt
    26521 tgtgttacca ggtcgataac ctttcgcaag cagcgttggt cagtcatatt cagcacatca
    26581 ataaactcaa gaccacgttc gagcatatcg tcacggttga atcagaactc cccaccgcgg
    26641 cacgttttga atgggtgcat cgtcatttgc cggggctgat cacccttaat gcttaccgca
    26701 cgctcaccgt tctgcacgat cccgccactt tacgctttgg ttgggctaat aaacatatca
    26761 ttaagaattt gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg aaatcaccac
    26821 gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa actggagcga gagtatcagg
    26881 atatcgctgc cctgccacag aatgcgaagt taaaaatcaa acgtccggtg aaggtgcagc
    26941 cgattgcccg cgtctggtac aaaggagatc aaaaacaagt ccaacacgcc tgccctacac
    27001 cgctgattgc actgattaat cgggataatg gtgcgggcgt gccggacgtt ggtgagttgt
    27061 taaattacga tgccgacaat gtgcagcacc gttataaacc tcaggcgcag ccgcttcgtt
    27121 tgatcattcc acggctgcac ctgtatgttg cagattaacg cccggctttc atactgccga
    27181 ccatctgttc tggccgtacc cagctgtcaa actcggcttc gctaagatac cccagcgcaa
    27241 gggccgcagc ttttaaggtc agcccttctt tatgcgcttt tttggcgatc tccgcggctt
    27301 tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat cagcgattca ttgagtaatt
    27361 gattgattcg ctcacgattc ggttcaatac ccactgcgca gtgtttgtta aaactttcca
    27421 tgccatctgc cagcaagcgc accgattgca ggaaattgtg gatcaccatt ggacggaaga
    27481 cgttcagttc aaagttaccg gaagcgcccc ccatgttgat cgccacgtcg ttccccatca
    27541 cctgacagca gagcatggtt aatgcctcac actgtgttgg gttcactttc cccggcatga
    27601 ttgagctgcc cggctcattt tccgggattg agatttcacc aattccgcag cgcgggccag
    27661 aggccagcca gcggacatca ttggcgattt tcatcagtga cgcagccaac cctttcaacg
    27721 cgccgtgcgc ctgaaccagg gcatcacagg tcgccagcgc ttcaaatttg ttcggcgcgg
    27781 taacaaacgg tgcacaggta atgactgcca gttcatctgc tacgcgacgc gcatactccg
    27841 gatgggtatt tagtccagta cccaccgctg taccgccaag agccagttcc gctacgtgag
    27901 gcaggctgta ttcgatatgt ttgagattat gctcgagcat cgctacccag ccggaaatct
    27961 cctgccccag tgttagcggg gtggcgtcct gcaagtgggt tcgaccgatt tttacgatat
    28021 cggcaaatgc acgcgatttt tcactcagcg tctgtgtcag ggttttaagc tgcggaatga
    28081 gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc cgtcggaaag acatcgttgg
    28141 aactttggct tttgttcacg tcgtcgttag ggtgaacttt acgttccatc ccgcgcacgc
    28201 cgccgagtaa ttcactggcc cggttagcca gcacttcatt catgttcatg ttactttgcg
    28261 tgccggagcc ggtctgccag atagccagcg ggaattcgtc gtcatgctgt cctgccagta
    28321 cttcatccgc cgcctgccga atggcgctcg ctttctcttc agacaacaag cctaaatctt
    28381 cattaacttt tgccgctgcg cgcttggtta gcgccagcgc atgaatcagt gaggtgggca
    28441 ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg ccccacagct
    28501 tatctgccgg gacatcaatc gcccccatcg aatctttttc gctgcgtact gtattcatgg
    28561 cctactcctc acctgattaa ttttttcttt ctgttttgct ttcgttaagt aacttttagc
    28621 tcactcatta tttactattt gataacaaat gtttggtctt tcgtgccatg taaaaaaacc
    28681 gccccgaagg gcggctctgt ttatttcaca cagcgggtgc attgtgtgag ttgtatctgc
    28741 tggaagaagt catttccttt atcatccaca aggataaacg ccgggaaatc ttccacttca
    28801 attttccaga tggcttccat tcccagttcc ggatattcaa cacattccag gctcttaata
    28861 cttccctgcg ccaatacagc ggccggacca ccgatactgc caaggtagaa gccaccgtgt
    28921 tttttacagg catccgtcac ctgctggctg cggttgcctt tcgccagcat gatcatactt
    28981 ccgcccagcg cttgcagttg atcgacataa gaatccatcc gtccggcggt cgttgggcca
    29041 agagaaccgg aggcataacc ttccggcgtt ttggccggac ccgcgtagta aatcggatga
    29101 tctttgatgt actgcggcag cccttcaccg ttatccatcc gctctttcag tttggcgtga
    29161 gcaatatcac gaccgacgat aatcgtgccg ttaagcgata agcgtgtaga aacgggatac
    29221 tgcgacaact gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg caccgcttcg
    29281 ccttctcccg ctttgcgcag ctcttccggg atatatttgc ctggattatg ttccagtttt
    29341 tcgatccaga tcccctgacg gttgatcttc gctttgatat tacggtcagc agaacaggag
    29401 acgcccatac cgaccgggca ggatgcgccg tgacgtggca ggcgaatcac gcggatgtcg
    29461 tgagcgaagt atttaccgcc aaactgcgca cccagaccaa gattttgcgc ttcgatcagc
    29521 aattcttttt ccaattccac atcgcggaac gcctgaccgt gctcattccc ttccgttggc
    29581 agttcatcat agtatttcgc ggaagccagt ttcaccgttt taaggttcgt ttctgcagaa
    29641 gttccaccaa taacgaacgc aatatgatac ggaggacagg ccgccgtacc cagcgtgcgc
    29701 atcttctcaa ccaggtaatt tttcagtttc cccggcgtca gtaacgcttt ggtttcctga
    29761 tagagatacg tcttgtttgc cgaaccacca cctttggcga tacagaggaa tttgtattcg
    29821 tcgccatcaa cggcataaag atcgatctgc gctggcagat tggtgccggt attcacttct
    29881 ttatacatat ccagcggcgc gttttgcgag tagcgcagat tatcttcgat ataagtgtta
    29941 tagacaccgc gcgccagcgc cgcttcatca ccaccaccgg tccatacacg ctgccctttt
    30001 ttaccaacaa taatcgcggt gccggtatcc tgacaggttg gcagaacgcc tttcgccgcg
    30061 atgtcggagt tacgcaggaa ttgcagcgcc acatatttat cattttcgct ggcctccggg
    30121 tcacgcagaa tgtcggccac ctgttgttgg tgcgccggac gcagcatgaa cgacgcatca
    30181 tgaaacgcct ggcgcgccaa cagagttaac gcttcgggtg cgactttcaa aatctcctgc
    30241 ccttcaaatt cagataagct aacgtgttcg ctggttagca ggtaatactc agtatcatct
    30301 tttttgagtg gaaaaggagc ctgataatga aagggtttgt ttgacattgt tttctcactt
    30361 actgcctggt ttggttatgc tctgggcggg tgttccgttg ccctgttaaa agcgagtaac
    30421 aatatcctac gcactttttt aacaaaaact gagactagta cgactttttg cggctccagg
    30481 ttacttcccg taggattctt gctttaatag cgggattaat ttccacatta aaacagggat
    30541 tgatcatgca aaaactcatt aactcagtgc aaaactatgc ctggggcagc aaaacggcgt
    30601 tgactgaact ttatggtatg gaaaatccgt ccagccagcc gatggccgag ctgtggatgg
    30661 gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc cggagatatc gtttcactgc
    30721 gtgatgtgat tgagagtgat aaatcgactc tgctcggaga ggccgttgcc aaacgctttg
    30781 gcgaactgcc tttcctgttc aaagtattat gcgcagcaca gccactctcc attcaggttc
    30841 atccaaacaa acacaagtct gaaatcggtt ttgccaaaga aaatgccgca ggtatcccga
    30901 tggatgccgc cgagcgtaac tataaagatc ctaaccacaa gccggagctg gtttttgcgc
    30961 tgacgccttt ccttgcgatg aacgcgtttc gtgaattttc cgagattgtc tccctactcc
    31021 agccggtcgc aggtgcacat ccggcgattg ctcacttttt acaacagcct gatgccgaac
    31081 gtttaagcga actgttcgcc agcctgttga atatgcaggg tgaagaaaaa tcccgcgcgc
    31141 tggcgatttt aaaatcggcg ctcgacagtc agcagggtga accgtggcaa acgattcgtt
    31201 taatttctga attttacccg gatgacagcg gcctgttctc tccgctgttg ctgaatgtgg
    31261 tgaaattaaa tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac gcttacctgc
    31321 aaggcgtggc gctggaagtg atggcaaact ccgataacgt gctgcgtgcg ggtctgacgc
    31381 ctaaatacat tgatattccg gaactggttg ccaatgtgaa gttcgaagcc aaaccggcta
    31441 accagttgtt gacccagccg gtgaaacaag gtgcagaact ggacttcccg attcctgttg
    31501 atgattttgc cttctcgctg catgacctta gtgataaaga aaccaccatt agccagcaga
    31561 gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt gtggaaaggt tctcagcagt
    31621 tacagcttaa accgggtgaa tcagcgttta ttgccgccaa cgaatcaccg gtgactgtca
    31681 aaggccacgg ccgtttagca cgggtttaca acaagctgta agagcttact gaaaaaatta
    31741 acatctcttg ctaagcttat taaaggctta taacaccttc aggcggccag tccgcctgat
    31801 ttcattttat ggataatcat tatgaataaa tcgctggtag cggtaggcgt cattgttgcg
    31861 ctaggcgtag tctggacagg cggcgcatgg tatacaggca agaagattga aacccatctc
    31921 gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga ccgctcctga atccaacctg
    31981 gaagtgagtt atcaaaacta tcatcgcggc gtattcagca gtcagctgca actgttggtg
    32041 aaacccattg ccggaaaaga aaatccgtgg attaaaagcg gtcagagcgt catcttcaac
    32101 gaatcggttg atcatggtcc cttccccctt gcccagctta aaaaactgaa cctgatcccg
    32161 tcgatggcat caattcaaac cacgctggtt aataacgaag taagcaaacc actgtttgat
    32221 atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca ttggttacag cggtgattcc
    32281 agttccgata tttcgctcaa gccactaaat tacgagcaaa aggatgaaaa agtcgccttt
    32341 agcggcggcg agttccagtt aaatgcggac agagacggca aagctatctc cctttccggg
    32401 gaggcgcaaa gtggtcggat agacgcggtt aacgaataca accagaaagt acagttgacc
    32461 tttaataatc tgaaaaccga cggttccagc acgctggcaa gttttggtga gcgcgtagga
    32521 aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg aaggcaaaga actggcactg
    32581 ctggaaggca tggagatcag cggtaaatcg gatctggtca atgacggtaa aacgatcaat
    32641 agccaactgg attactcgct aaacagcctg aaggtacaga atcaggatct gggcagcggc
    32701 aagctgactt taaaagtcgg ccaaattgat ggcgaagcct ggcatcagtt tagccagcaa
    32761 tataacgcgc aaactcaggc gctgctggca cagccagaaa ttgccaacaa tcccgaactt
    32821 tatcaggaga aagtgacgga agccttcttt agcgccctgc cgctgatgtt gaaaggcgat
    32881 ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc agggtgaaag tgcgctgaat
    32941 ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag cgccgcaaac gctggcgcag
    33001 gaagtagatc gttcggttaa atctctggat gcgaaactga ccattccggt ggatatggca
    33061 actgagttga tgactcaggt agcgaagctg gaaggttatc aggaagatca agcgaaaaaa
    33121 ctggcgaaac agcaagttga aggtgcatca gcaatggggc agatgttccg tctgaccacc
    33181 ttgcaggaca ataccatcac caccagcctg caatatacta acggtcagat aacgttaaac
    33241 gggcagaaaa tgccactgga agatttcgtt ggtatgtttg caatgccggc attaaatgtt
    33301 ccggtcgtac ccgctattcc gcagcagtaa ttcacagcca aaacgccgga gatttttctc
    33361 tccggcgtta ttttttactt cagcataaag tcatactgaa tactcatttt cacatcccag
    33421 taattcaggc gatcgcccag cgtactgcca tcgcgctttt cccgcccttt atcggcagca
    33481 agaaagaaat tcatcccttg taacggccct tcgctgaccc gatagttcat accacctaag
    33541 atacccgcgc cttcaatccg cacatctcgt ttactggatt catagccgcg agtcacaatc
    33601 ggtgcaaatg tcagtgtaaa ttgggagtta aacgatagtt gacgcccaat tgccaggact
    33661 gcattccttc acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca
    33721 ccacggcacc attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg
    33781 gcgcattcca ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac
    33841 gacttaaacc ttcgagttgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg
    33901 ggtcgagcgg tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta
    33961 ccgatgcccc cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac
    34021 gagcgcgatg tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac
    34081 cggttgttga atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat
    34141 ttggcgttaa agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg
    34201 aactgccagc cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga
    34261 agcgacctaa tttaagcagg ctattttcac tgggttttat ttttactgcc ccgagggcaa
    34321 agccaaaact tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta
    34381 atttataaac ataatacgct ccgccttcaa cgccaataat attggaataa taaccactat
    34441 tgaaatcgag caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg
    34501 gcttatcagc cctgcgcaat tcgtttttca gtctgatgcg tagtcccgct tcatcgtcag
    34561 ccagttgcgc cgcgtaagca gaggtaaggc cagaggcagc cgtcaggcaa ataacggcca
    34621 tggccactat ttttctcata gttgcacctt tgatgttctg attattttta ttgaatatta
    34681 attagtgata tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac
    34741 gatttctttg aattttttat ccgtgagcgg ataaaaccag ataataacga atgccagtag
    34801 cataaatccg caaggtacta aggcaattga tgtgcggatg cccataatta cttccggcgt
    34861 ttgcacctga ttggcgatat atccgcttaa tcccaaaata aaggcaggaa ttgaaccacc
    34921 gattgcctga ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg
    34981 cacgccggtc aggtattcac cgtattctac ggtatcagct tccagcgccc acatcacggt
    35041 catggtaacg ccctgaccaa ttgaggcaat ggctaacgca accaacgcca ccggcagcga
    35101 ccagacggaa acccagaaga acagcagata accgcaggtt cccagcaaag cgccaatcag
    35161 gaaggtattc tttttaccga tcctcgcgac catccccggc accaacggtg ccgatgccac
    35221 agtaccaacc aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac
    35281 atagcgcacg tagaacaacg acgaggcgct gaccgcgaag gtcgaaatca gcacacacag
    35341 cgcaccgatg cacaacataa acagcgggcg attccgtttc agggtttgca gactgatctt
    35401 caatgacggc tgcgccacga tacgtaccac attctcacgc gtcgatttga agcagatgaa
    35461 gtaaagcacc attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat
    35521 ctcttccggg ctggagttct taatgctcgg ccctatcaga aatgccaggc agacaaaggt
    35581 caatgaagcg gcaatcccac gagccgcgcc cagacgggcg cgggattgtg gttgttgggt
    35641 catcgcggta gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc
    35701 catgtaggtc aaatatgcat acaccacttt gctaccatgg ctccagtcgg tcggcaccca
    35761 gaataccagc acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa
    35821 ttttccccag cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc
    35881 atcgaatacc cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac
    35941 gtcggtgtag taactcaaca ggaagagcgc ccccattgcg aaggcgaagt tattggcgat
    36001 gtcaccaagg ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct
    36061 ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc
    36121 gccgacttcg gtttgcggtc gcgggtgaag atccccttct tgttaccgcc aacgcgcaat
    36181 atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg
    36241 acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac
    36301 atgtcggtgt acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata
    36361 atcggctgat gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc
    36421 gtttccaaat cgccgctttg gacataccaa ccgtaataac ggttcaggca cagcacatca
    36481 aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc
    36541 ggacgcgtcg gatcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca
    36601 ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt
    36661 ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cctgctgagt ttccccgttg
    36721 actgcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa
    36781 gagaggttaa agccgacagc tgcagtttca tcaatcacca cgatgccatg ttcatctgcc
    36841 cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc
    36901 cagtccatta atgcgtgatc gtgcaccatc agcacgttat cgaatccttt gccgcgcaaa
    36961 tccgcatctt catgacggcc aaagccagta aagtagaacg gtttgtggtt gatcaggaac
    37021 tgttcgccct tcactgccac tgaccggatg ccgacgcgca gcgggtagat atcacactct
    37081 gtccggcttt tggctgtgac gtacagttca tagagataac cttcacccgg ttgccagagg
    37141 tgcggattca ccacttgcaa agtcccgctg gtgccccttg tccagttgca accacctgtt
    37201 gatccgcatc acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag
    37261 acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt
    37321 tcggcgtggt gtagagcatt acgctgcgat ggatcccggc gtagttaaag aaatcatgga
    37381 agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct
    37441 gccagttcag ttcgttgttc acacaaactg tgatacgtac acttttcccg gcaataacat
    37501 acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat
    37561 tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc
    37621 ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc acataattac
    37681 gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt
    37741 cttgtaacgc gctttcccac caacgctgct caattccaca gttttcgcga tccagactga
    37801 atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca
    37861 taagggactc ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa
    37921 ttctgtgttg cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga
    37981 actgacaccg cgatcaaaaa accaaaaact gcaataccca cagcgtgata tagatcgcat
    38041 taatctttaa aacggcgttg acagaagcca taaaataagc aaccgaacgt tcgttcgagc
    38101 atgttgggaa cgataaactc tcatgacata tggctacggt gaatatttta gggaggaatt
    38161 ccccgctccg gcgagattgt attttgtaca gggaaattaa agtatgaaac gcgggctcag
    38221 ttgtcaccag cccgcggaaa gagagagaga atcaggatgc ggttaagata ccgccaatca
    38281 tcgcgcgtaa tccctgagca aacgacgctt cacgcgccag gtcttccgct tcgatatcgg
    38341 ccagtacacc gtaggtcaga tccagcagta aacgtgaagt cattgccgtg ttgatgtctg
    38401 ggcttatctc gcctttttgc tgggcatcac gcatccgctg agcaacaaat tcagtgatcg
    38461 tcatatgctt atttttaagc atggcggcga cctgtgggtt acgcatccct tccgccataa
    38521 tttcgaccac cagcgcccgt tgcccaaagg cttcatgggt gagagagaca atagactcga
    38581 ccatatagtc aacaaaatga atcccttcaa tcggttcccg gaaacgggcc agcgccctct
    38641 cctggtcctg taagataatg gcctgaatca aggcttcttt ggagatgaaa tggtgataga
    38701 gcgtcccggg actaatggcg caggatttac agatggcttt catcgaggca ctgtgaaatc
    38761 cattttctga aaaaatctct ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt
    38821 cagtctgcat gttatccatc attaaaccat tatagttagc gtgaataact agtttaccgc
    38881 gttgtcaggt gagcgcctat cattgctgtt gaatagtgcg aaggcacact ctattcgtat
    38941 gcttttcatc cagctattgg ttaaccaatg gccgtgattt ggctgccagt tgtttagcag
    39001 agacgttgat gaattgcaca cgctatcccg gtaaacaaca catacggctg tttaccggtt
    39061 cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc
    39121 ttacccagct ggctgcaggc gagcaaagga acagcgctgc gttagcaata tcttgcggtt
    39181 ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa
    39241 cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccattcacc cgaatatttt
    39301 tttcacccag gtcaaatgcc atatttctga ccagatgact ggccgcagct ttagatgatg
    39361 cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa
    39421 cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa
    39481 acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac ggtttaggtc
    39541 caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg atagcaaagt
    39601 ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgac
    39661 cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga
    39721 ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac
    39781 ccgcacctgt gatgatggcg cattttccgt cgagtctcag gttgtcagaa ttaaacacat
    39841 aaacctcctg tgaacttcag taaaaataaa aggcaccgcc tgtgtgccac attttttatc
    39901 attaaacttg aggaaaggac actaccaggt atagcagata aagagaggat tatttcgccg
    39961 caatgagccg ggcgggaata ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt
    40021 gcatcatgcg atccgcaagt gtggtaccaa gttcccgcgc aggcgtgctg gcccaggtaa
    40081 cgggtatatc atcaagtgtg gttggtgtcg catcggtaaa tgccgccagc gaaacctgtt
    40141 gctcaaagta acgatcgact ccgctttccc cgctttgccg ccctgctttc agcaaaccaa
    40201 accatgcccc catcgcaata gtttcgttat agcaaaccac ggcactgatg gtcgggttat
    40261 gacgtaaaag cgccgtgata gcttccgcgg cttgcttctg gctggaagtg cactccaaca
    40321 cccaatcgct gtgaaacggc aggccaaatt ttagtagagt tgcacaatag ccccccaccc
    40381 gttctgcacg ggttaatgag gaactttgcc ctcccagcca ggcgatccgc tgatgcccat
    40441 tgcgaatgag atgctccgtc aacaactgtg cagcctgcat gttgtccggg cgaaccgtat
    40501 caacatcatc aagataactg gcacgggaag cgaaaatcac cgggatagct ttttcttctg
    40561 ccatccgtcg caggtcatcg ctgcttcctg cagccccggc aattaccaca ccatcgacac
    40621 cctgattcag taacagtgaa aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa
    40681 gcaaaaaaac catccgtccc tgcgcttcca gagcttccgt caatccggcc gtcaattcgg
    40741 cgtaaaacgg cgcagataaa tcacggacga tcaaaccaat gacgccgctt tgcccaccgc
    40801 gcagcgccga cgcctggcga ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc
    40861 gttctcctgt ggcggtagag attcgccctt tgccactaag caccagcgaa acggtactta
    40921 ccgacacgcc cgcagccagc gcaacatcat gaatggttat ttttttggcg gtagccatgc
    40981 agatgaccta ctccctgatt ttgtgacaga taaaacgttt taccttttat ttatcttata
    41041 cccgctatta tcgttgcgta atgtgattta tgcctcacta aaatttgata aaacgtttta
    41101 tcttctcgcg caatttactg aatccagatt gttctctacg aggagtcgtt ttatgacggc
    41161 gaaaacagca ccgaaagtca cgctgtggga gttcttccag cagttaggca aaaccttcat
    41221 gttacccgtg gcattattgt cgttctgcgg cattatgctc ggcattggta gttcccttag
    41281 tagccacgat gtcatcaccc tgctcccggt cctgggcaac cccgtgttgc aggctatctt
    41341 tacctggatg agtaagattg gctcgtttgc ttttagtttc ctgccagtca tgttctgtat
    41401 cgccatcccg ctgggtctgg cacgagaaaa caaaggcgta gcggcattcg ctggcttcgt
    41461 cggttatgcg gtaatgaacc tcgcggtaaa cttctggttg accaataaag gcattctgcc
    41521 aaccacggat gccgccgttc tgaaagccaa taacatccag agcattcttg ggatccagtc
    41581 gatcgacacc gggatcctcg gtgcggtgat cgccggtatt atcgtctgga tgctgcatga
    41641 gcgattccat aatatccgcc tgccggatgc gctggcattc ttcggcggta cgcgcttcgt
    41701 accaatcatc tcctcgctgg tgatgggcct tgtcggcctg gtgattccat tagtctggcc
    41761 gattttcgcc atgggtatta gcggcttagg ccatatgatc aatagcgcgg gtgatttcgg
    41821 accgatgctg tttggtaccg gtgaacgtct gctgttgccg tttggtctgc atcacattct
    41881 ggtggcatta attcgcttta ccgacgcagg tggcacgcag gaagtctgcg gtcaaaccgt
    41941 cagcggcgca ttgaccatct tccaggcgca attgagttgc ccgaccactc acggtttttc
    42001 tgaaagcgcc acgcgtttcc tttcgcaagg caaaatgcct gcgtttctcg gcggtctgcc
    42061 aggtgcagcg ttagcgatgt atcactgcgc gcgcccggaa aatcgccata aaattaaagg
    42121 gctgctgatt tctggcctga tcgcctgtgt cgttggcggc acgaccgaac cgctggaatt
    42181 cctgttcctg ttcgtagcgc cagttctgta tgtcatccac gcgctgttaa ccggcctcgg
    42241 cttcaccgtc atgtctgtgc tcggcgtcac catcggtaat accgacggca atatcatcga
    42301 cttcgtggtg ttcggtattt tgcatggtct gtcaaccaag tggtacatgg tgccagtggt
    42361 ggtggcaatc tggtttgtcg tttactacgt catcttccgt ttcgctatca cccgcttcaa
    42421 tctgaaaacc ccggggcgcg atagcgaagt ggccagttca atcgaaaaag ccgttgccgg
    42481 tgcgccgggt aaatcaggtt acaacgttcc ggcaatcctc gaagccttag gcggtgccga
    42541 caatattgtt agcctcgata actgcattac ccgtctgcgt ttgtctgtga aagatatgtc
    42601 gcttgttaat gtgcaggcac tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca
    42661 acataacctg caggttgtta tcgggccaca agttcagtca gtaaaagatg aaatggccgg
    42721 tctgatgcat actgtccagg cataaggata agatatgttc gatttttcaa aggtcgtgga
    42781 tcgtcatggc acatggtgta cgcagtggga ttatgtcgct gaccgtttcg gcactgctga
    42841 cctgttacct ttcacgattt cagacatgga ttttgccact gccccctgca ttatcgaggc
    42901 gctgaatcag cgcctgatgc acggcgtgtt tggctacagc cgctggaaga acgatgagtt
    42961 tctcgcggct attgcccact ggttttccac ccagcattac accgccatcg atcctcagtc
    43021 ggtggtgtat ggcccttctg ttatctatat ggtctcagaa ctgattcgtc agtggtctga
    43081 aacaggtgaa ggcgtggtga tccacacacc cgcctatgac gcattttaca aggccattga
    43141 aggtaatcag cgcacagtaa tgcccgttgc tttagagaag caggctgacg gctggttttg
    43201 cgatatgggc aagctggaag ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg
    43261 tagcccacag aatcctaccg gaaaagtgtg gacgtgcgat gagctggaga tcatggctga
    43321 cctgtgcgag cgtcatggtg tgcgggttat ttccgatgaa atccatatgg atatggtttg
    43381 gggcgagcag ccgcatattc cctggagtaa tgtggcgcgg ggagactggg cgttgctgac
    43441 gtcgggatcg aaaagtttca atattcccgc gctgaccggt gcttacggga ttatagaaaa
    43501 tagcagcagc cgtgatgcct atttatcggc actgaaagga cgtgatgggc tttcttcccc
    43561 ttcggtactg gcgttaactg cccatatcgc cgcctatcag caaggtgcac cgtggctgga
    43621 tgccttacgc gtctatctga aagataacct gacgtatatc gcagataaaa tgaacgccgc
    43681 gtttcctgaa ctcaactggc agatcccgca atccacttat ctggcctggc ttgatttacg
    43741 tccgttgaat attgacgaca acgcgttgca aaaagcactt atcgaacaag aaaaagtcgc
    43801 gatcatgccg gggtatacct acggtgaaga aggtcgtggt tttgtccgac tcaatgccgg
    43861 ctgcccacgt tcgaaactgg aaaaaggtgt ggctggatta attaacgcca tccgcgctgt
    43921 tcgctaaccc caattgcgca acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc
    43981 tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc atgattgata
    44041 ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt ccccagacca
    44101 ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctg gaaacactga
    44161 ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg accaaacttg
    44221 actggggcgt taaagttctc gcctctcttg atgcttgtcg ccgcgtggca tttgaaaaca
    44281 ttgaagatgc agcccgtaac ggcctgcact atgtcgagct gcgtttttca ccaggctaca
    44341 tggcaatggc acatcagctg cctgttgcag gtgttgttga agcggtgatc gatggcgtac
    44401 gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg agccgaacct
    44461 tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt gaccagatta
    44521 ccgcacttga tttagccggt gatgaacttg gtttcccggg aagtctgttt ctttctcact
    44581 tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc
    44641 cggaaagcat ctggcaggcg attcgtgaac tgggggcaga gcgcattgga catggcgtaa
    44701 aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt ggtattgaat
    44761 cctgtctgac ctccaatatt cagaccagca ccgtggcaga gctggctgca catccgctga
    44821 aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc ggcgtacagg
    44881 gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta tcccgcgagc
    44941 aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcaacgca gaggaaaaac
    45001 gcgcactgcg agaaaaagtc gcagcgaagt aacaaaaatg gatggtgcaa attgcaccat
    45061 ccatttttca tgcaaggcac aaagtcgcgc gatgtttggc ggattcgatg cccagctcaa
    45121 tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca tcgccattta
    45181 acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc aacagcgttt
    45241 cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata tcgtagcccc
    45301 agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga tcgaggccat
    45361 atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca gcggctgcca
    45421 gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg tggaaataat
    45481 cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg accggtaaac
    45541 caaatagcgt aatggcctga tcaagaagat gtggtgctaa atcgtaccag ataccgctgc
    45601 ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg tcaaaatgag
    45661 actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt agcgtcaaga
    45721 aatcgctatc ccaacgacgg ttatggaata cagacagaac acgccccagg ctttttgcca
    45781 gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca acgaccacat
    45841 gtttacccgc ctcaagcgct gctttggcta acgggaaatg ggtatcgttg ggtgtaggaa
    45901 tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag acaaccgtaa
    45961 ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc gccagttcca
    46021 gcccgggtgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa ccatacccaa
    46081 tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta tgatggctat
    46141 ttgacactat cctttaccct cgctcaacag tttaataacc tgccagcaat aagggatgtt
    46201 gtttaactta agtcaaaaaa atagcgattt ttccaacgac aaaagctaaa tatcgcaaaa
    46261 acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc cctgaagtta
    46321 atggatcaat caacagagat gtgagttgta atgaatcgtc ttattgaatt aacaggttgg
    46381 atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga caactatcag
    46441 ccacctgaac agagtactat ggtacaacac aagtaagccc tgcgcttgta gagcgacatg
    46501 ctgcccgtca gggtgcatgt tttcacttgt cggatattaa accaggaatt tattatcttg
    46561 ttcggtgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc atagcagttt
    46621 ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac aagcgttcac
    46681 t
//
LOCUS       DAZFKY010000013        46275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFKY010000013 DAZFKY010000000
VERSION     DAZFKY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..1688
                     /gene="yegH"
                     /locus_tag="ACHKCL_000677"
     CDS             105..1688
                     /gene="yegH"
                     /locus_tag="ACHKCL_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5761717.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1763..2101
                     /locus_tag="ACHKCL_000678"
     CDS             1763..2101
                     /locus_tag="ACHKCL_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5761718.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2091..2381
                     /locus_tag="ACHKCL_000679"
     CDS             2091..2381
                     /locus_tag="ACHKCL_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5761719.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2434..4287)
                     /gene="asmA"
                     /locus_tag="ACHKCL_000680"
     CDS             complement(2434..4287)
                     /gene="asmA"
                     /locus_tag="ACHKCL_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5761720.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4309..4890)
                     /gene="dcd"
                     /locus_tag="ACHKCL_000681"
     CDS             complement(4309..4890)
                     /gene="dcd"
                     /locus_tag="ACHKCL_000681"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5761721.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4982..5623)
                     /gene="udk"
                     /locus_tag="ACHKCL_000682"
     CDS             complement(4982..5623)
                     /gene="udk"
                     /locus_tag="ACHKCL_000682"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5761722.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5941..9259
                     /gene="dgcE"
                     /locus_tag="ACHKCL_000683"
                     /pseudo
     CDS             5941..9259
                     /gene="dgcE"
                     /locus_tag="ACHKCL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9360..10208)
                     /gene="alkA"
                     /locus_tag="ACHKCL_000684"
     CDS             complement(9360..10208)
                     /gene="alkA"
                     /locus_tag="ACHKCL_000684"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5761723.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10342..11694
                     /gene="yegD"
                     /locus_tag="ACHKCL_000685"
     CDS             10342..11694
                     /gene="yegD"
                     /locus_tag="ACHKCL_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5761724.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11707..13647)
                     /gene="yegI"
                     /locus_tag="ACHKCL_000686"
     CDS             complement(11707..13647)
                     /gene="yegI"
                     /locus_tag="ACHKCL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5761725.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13644..14405)
                     /gene="pphC"
                     /locus_tag="ACHKCL_000687"
     CDS             complement(13644..14405)
                     /gene="pphC"
                     /locus_tag="ACHKCL_000687"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO5761726.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14402..15061)
                     /gene="yegL"
                     /locus_tag="ACHKCL_000688"
     CDS             complement(14402..15061)
                     /gene="yegL"
                     /locus_tag="ACHKCL_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5761727.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15282..15341)
                     /gene="ibsA"
                     /locus_tag="ACHKCL_000689"
     CDS             complement(15282..15341)
                     /gene="ibsA"
                     /locus_tag="ACHKCL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5761728.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15613..15669)
                     /locus_tag="ACHKCL_000690"
     CDS             complement(15613..15669)
                     /locus_tag="ACHKCL_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5761729.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15941..15997)
                     /locus_tag="ACHKCL_000691"
     CDS             complement(15941..15997)
                     /locus_tag="ACHKCL_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5761730.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16277..17524
                     /gene="mdtA"
                     /locus_tag="ACHKCL_000692"
     CDS             16277..17524
                     /gene="mdtA"
                     /locus_tag="ACHKCL_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5761731.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17524..20646
                     /gene="mdtB"
                     /locus_tag="ACHKCL_000693"
     CDS             17524..20646
                     /gene="mdtB"
                     /locus_tag="ACHKCL_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5761732.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20647..23724
                     /gene="mdtC"
                     /locus_tag="ACHKCL_000694"
     CDS             20647..23724
                     /gene="mdtC"
                     /locus_tag="ACHKCL_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5761733.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23725..25140
                     /gene="mdtD"
                     /locus_tag="ACHKCL_000695"
     CDS             23725..25140
                     /gene="mdtD"
                     /locus_tag="ACHKCL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761734.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25137..26540
                     /gene="baeS"
                     /locus_tag="ACHKCL_000696"
     CDS             25137..26540
                     /gene="baeS"
                     /locus_tag="ACHKCL_000696"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5761735.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26537..27259
                     /gene="baeR"
                     /locus_tag="ACHKCL_000697"
     CDS             26537..27259
                     /gene="baeR"
                     /locus_tag="ACHKCL_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5761736.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27450..27782
                     /gene="yegP"
                     /locus_tag="ACHKCL_000698"
     CDS             27450..27782
                     /gene="yegP"
                     /locus_tag="ACHKCL_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5761737.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27930..29291
                     /gene="yegQ"
                     /locus_tag="ACHKCL_000699"
     CDS             27930..29291
                     /gene="yegQ"
                     /locus_tag="ACHKCL_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5761738.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29621..29938)
                     /gene="yegR"
                     /locus_tag="ACHKCL_000700"
     CDS             complement(29621..29938)
                     /gene="yegR"
                     /locus_tag="ACHKCL_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO5761739.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            30344..31243
                     /gene="yegS"
                     /locus_tag="ACHKCL_000701"
     CDS             30344..31243
                     /gene="yegS"
                     /locus_tag="ACHKCL_000701"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5761740.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(31325..32098)
                     /locus_tag="ACHKCL_000702"
     CDS             complement(31325..32098)
                     /locus_tag="ACHKCL_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5761741.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(32204..33244)
                     /gene="gatD"
                     /locus_tag="ACHKCL_000703"
     CDS             complement(32204..33244)
                     /gene="gatD"
                     /locus_tag="ACHKCL_000703"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5761742.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(33292..34647)
                     /locus_tag="ACHKCL_000704"
     CDS             complement(33292..34647)
                     /locus_tag="ACHKCL_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5761743.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(34651..34935)
                     /gene="gatB"
                     /locus_tag="ACHKCL_000705"
     CDS             complement(34651..34935)
                     /gene="gatB"
                     /locus_tag="ACHKCL_000705"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5761744.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(34966..35418)
                     /gene="gatA"
                     /locus_tag="ACHKCL_000706"
     CDS             complement(34966..35418)
                     /gene="gatA"
                     /locus_tag="ACHKCL_000706"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5761745.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(35428..36690)
                     /gene="gatZ"
                     /locus_tag="ACHKCL_000707"
     CDS             complement(35428..36690)
                     /gene="gatZ"
                     /locus_tag="ACHKCL_000707"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5761746.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(36719..37573)
                     /gene="gatY"
                     /locus_tag="ACHKCL_000708"
     CDS             complement(36719..37573)
                     /gene="gatY"
                     /locus_tag="ACHKCL_000708"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5761747.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(37872..38924)
                     /gene="fbaB"
                     /locus_tag="ACHKCL_000709"
     CDS             complement(37872..38924)
                     /gene="fbaB"
                     /locus_tag="ACHKCL_000709"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5761748.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            39181..40458
                     /gene="yegT"
                     /locus_tag="ACHKCL_000710"
     CDS             39181..40458
                     /gene="yegT"
                     /locus_tag="ACHKCL_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5761749.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            40455..41459
                     /gene="yegU"
                     /locus_tag="ACHKCL_000711"
     CDS             40455..41459
                     /gene="yegU"
                     /locus_tag="ACHKCL_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5761750.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            41456..42421
                     /gene="yegV"
                     /locus_tag="ACHKCL_000712"
     CDS             41456..42421
                     /gene="yegV"
                     /locus_tag="ACHKCL_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5761751.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(42395..43141)
                     /gene="yegW"
                     /locus_tag="ACHKCL_000713"
     CDS             complement(42395..43141)
                     /gene="yegW"
                     /locus_tag="ACHKCL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5761752.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(43193..44011)
                     /gene="yegX"
                     /locus_tag="ACHKCL_000714"
     CDS             complement(43193..44011)
                     /gene="yegX"
                     /locus_tag="ACHKCL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5761753.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(44076..44876)
                     /gene="thiD"
                     /locus_tag="ACHKCL_000715"
     CDS             complement(44076..44876)
                     /gene="thiD"
                     /locus_tag="ACHKCL_000715"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5761754.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(44873..45661)
                     /gene="thiM"
                     /locus_tag="ACHKCL_000716"
     CDS             complement(44873..45661)
                     /gene="thiM"
                     /locus_tag="ACHKCL_000716"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5761755.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(45696..45792)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45995..46234
                     /locus_tag="ACHKCL_000717"
     CDS             45995..46234
                     /locus_tag="ACHKCL_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO5761756.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT        10997 a        12225 c        11830 g        11223 t
ORIGIN      
        1 aaataatatg agataaatag tgctgcaaca ttgcattttt gccccgattt atccatgatc
       61 gaattgtgac atttgtcata caacgaatag gttttgtact tactatggaa tggattgccg
      121 atccgtctat ctgggccggg ttaatcacgc tgattgtgat cgaactggtc ctcggcattg
      181 ataacctggt ctttattgcc atcctcgccg aaaaactacc gccgaagcag cgcgaccgcg
      241 cacgggttac cgggctgttg ctggcgatgt taatgcgcct gttactgctg gcgtcaatct
      301 cctggctggt caccctgact caaccgctgt tcagcttccg ctcgtttacc tttagcgccc
      361 gcgacttaat catgctgttt ggtggtttct tcctgttgtt caaagccacg atggagctga
      421 acgaacggct ggaagggaaa gacagcaata atcccacaca acgcaaaggt gcgaagttct
      481 ggggggtggt gacacaaatt gtggtactgg acgccatctt ctcactcgac tcagtgatta
      541 ccgccgtcgg gatggtcgac catttactgg tcatgatggc cgccgtggtt atcgctatca
      601 gcctgatgtt gatggccagc aagccgttaa cgcaatttgt taacagtcac ccgaccatcg
      661 ttattctctg cttaagcttc ctgttgatga ttggctttag cctggtggca gaaggtttcg
      721 gcttcgtcat tccgaaaggc tacctgtacg ctgccattgg tttctcggtg atgatcgagg
      781 cgctcaatca gttggctatc tttaaccggc gacgttttct ttccgctaac cagacactgc
      841 gccagcggac taccgaagcg gtaatgcgcc tgcttagcgg gcaaaaagag gatgcggaac
      901 tggacgccga aaccgcgtcc atgctggtgg atcatggtaa ccagcagatc tttaatccgc
      961 aggaacggcg gatgattgag cgggtactta atcttaacca gcgtaccgtc agcagcatta
     1021 tgacgtcgcg ccacgatatt gagcatatcg atctcaacgc gccggaagac gagatccgcc
     1081 agttgctgga gcgaaatcag catacgcggc tggtggttac tgatggcgat gacgcggaag
     1141 atttgctcgg tgttgttcac gttatcgacc tgttacaaca atcactgcgc ggcgaaccgc
     1201 tcaacctgcg ggtgttaatt cgccagccgc tggtgttccc ggaaaccttg ccgttgttac
     1261 ctgccctgga gcagttccgt aatgcccgca cacactttgc ttttgtggtg gatgagtttg
     1321 gctcggtgga agggattgtg acattaagtg acgtcactga aaccattgcc ggtaacttac
     1381 cgaacgaagt ggaagagatc gacgcccgcc atgatattca gaagaatgcg gacggttcct
     1441 ggacggcgaa tggtcatatg ccgctggaag atctggtgca atatgtgccg ctgccgctgg
     1501 atgaaaaacg tgaatatcac accattgccg ggctattgat ggaatatttg cagcgtattc
     1561 caaaacctgg cgaagaagtt caggtggggg attatttgct taaaacgttg caggtggaaa
     1621 gccatcgcgt gcagaaggtg cagattatac cgctgcgtaa ggatggcgag atggagtacg
     1681 aggtgtgata aaacatcaaa tgcgcaggtt caaactgcgc atttgattta tacaaaattt
     1741 agtataatct aaggatggag taatgagaaa aaagctggct tttcttgata ccagtcttga
     1801 cgatttacgc gcatttccag aatcgtctcg acaggaaatt ggctatcaac tcgacaggat
     1861 ccaacaaggg ttaaacccat acgactggaa acctttttca actatcggcc ctggtgtgcg
     1921 agagattcgt actcgcgatg ctgatgggat ctatcgcgtc atgtatgtcg caaaatttga
     1981 agaagcggtt tacgtattgc attgttttca aaagaaaaca cagactacca gtcaatcgga
     2041 tattgattta gccaaacgac gctataaaga acttgttcag gagcggaaaa atgaaaattg
     2101 aaacctttga tagtgtgtgg gatgcggtga gtgacacccc tgaacaagcg gaaaatatgc
     2161 ggatccgtgc cgagcttgta acgatcatta ataactggat tgaacaacaa gggtttagcc
     2221 aggctcaggc agcttctgct ttaggggtca ctcaacctcg catctctgaa cttgccagag
     2281 gtaaaatcca gatctttagt atcgataaat tgatcaccat gatggctcat gcaggactac
     2341 atattcaacg tattgaagtt caatatccac atgctgctta gtgctataaa tacagatggc
     2401 gacaaattga tgccgccatc tatataactt gtcttacatc ttctccagca acttcttcac
     2461 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     2521 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc agactgtaat tgagttgctg
     2581 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
     2641 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
     2701 cagattcaac atgccttccc ctgtaagcgc cagcacaggc gattgacctt gcatgtcgtt
     2761 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gacgcgttac
     2821 gttatcgaag ttttcagcgg ccttcacatc accaccatta cgttctaccg cttgctgaat
     2881 catctgctgg aagttcatcc cttccatgcg cgtatcggcc atttcgacat gtgcctgtcc
     2941 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccaa
     3001 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg tgccaatctc
     3061 aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
     3121 gcccggcagt gaaacctgtc caccgttaag cttgccctgt agttgagtaa tttccagcaa
     3181 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
     3241 gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc
     3301 atcaatacgc gaagaaataa ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
     3361 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
     3421 cgggaattgc agcctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc
     3481 gctcagcgta ctatcattgg cggtcaaact aatttggtta aatgaaaggc gtttacgtga
     3541 ctcctgccac tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
     3601 accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg gataatcaga
     3661 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
     3721 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
     3781 gattgtcacc tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag
     3841 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
     3901 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
     3961 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
     4021 cacgtccagg cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag
     4081 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
     4141 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
     4201 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
     4261 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
     4321 gattcggctg gctaccgcgc cctgctggtt gcgatacttc gcatcttcac ggcggttgta
     4381 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
     4441 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
     4501 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccagtgagga
     4561 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
     4621 cagcgccagc tctcctgggt gaagatagaa cgcctcgctt tcatcgagaa cgatctcatc
     4681 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
     4741 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
     4801 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
     4861 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct
     4921 taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag
     4981 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
     5041 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
     5101 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
     5161 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
     5221 agaagttaag ttcgtcacgc aaacgtgcat ccgtcagcaa caaaatgcct tcgagaatga
     5281 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
     5341 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcagcaggc
     5401 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
     5461 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
     5521 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
     5581 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
     5641 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
     5701 tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacgaagaa
     5761 ttcccggtgg caaattacgt tgatcagttt tatacaaggt aaaaattgtt atacgcagtt
     5821 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
     5881 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
     5941 atgagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
     6001 agtttaggtt tagtctcgtt tatcttcacc cttttctcgc ttgagctttc gcagtttggc
     6061 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
     6121 gccgggcgca tgtggccagg gattgcgctg agctgctcgc tgggaaatat cgccgcatcc
     6181 atcctgcttt tttccaccag ctcgctgaac atgacctgca cgaccatcaa tattgttgaa
     6241 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
     6301 ctggctgact ggctgcgtct ggcactcggc agcgccattg ttccacctct gttggggggt
     6361 gttctggttg tcctgctgac gcccggagac gatcctcatc agggcatttt tgatatgggt
     6421 actgtcagaa tccatcggcg ctctggcact ggtgccgctg ggattgttat ttaaaccaca
     6481 ctatctgctg cgccatcgca acccacggtt gctttttgag tcgctgctca cgttagccat
     6541 cacactgacg ttaagctggc tttcgatgct gtatctgccg tggcctttta ctttcattat
     6601 tgtgctgttg atgtggagcg ccgtgcgcct gccacgaatg gaagcctttt tgatcttcct
     6661 taccacggtg atgatggtgt cgctgatgat ggccgcggat ccctccctgc ttgctacgcc
     6721 gcgtacgtac ctgatgagcc atatgccgtg gctaccgttt ttgctgatcc tgctgcccgc
     6781 caacatcatg acgatggtga tgtatgcctt tcgtgcggaa cgcaaacaca tttccgaaag
     6841 cgaaacccgt tttcggaacg ccatggaata ttccgccatc ggcatggcat tagtgggcac
     6901 cgagggacaa tggctgcaat ccaacaaagc actctgccag tttctcggtt acagtcagga
     6961 agagctgcgc ggactcacct ttcagcaact gacctggccg gaggatctca ataaagatct
     7021 ccaacaggtt gaaaagctga taagcggtga aataaacacc tattcaatgg aaaaacgtta
     7081 ctacaaccgc aatggcgatg ttgtctgggc gttgcttgcc gtctctctgg tgcgccacac
     7141 ggatggcacg ccgctctatt ttatcgctca gattgaagac attaacgagc taaaacgcac
     7201 cgaacaggtg aatcagcaac tgatggaacg catcacgctg gctaacgaag cgggcgggat
     7261 tggcatctgg gagtgggaac tgaagccgaa tatttttagc tgggataagc ggatgttcga
     7321 gctgtatgaa attcctccgc atatcaaacc gaactggcag gtgtggtacg agtgcgtgct
     7381 gccggaagat cgccaacacg ccgaaaaagt gattcgtgat tcgttgcaat cacgctcgcc
     7441 ctttaaactg gaatttcgca ttgccgtgaa agatggcatc cgccatatcc gcgccctcgc
     7501 caaccgggta ctgaataaag aaggcgaagt cgaacgcctg ctcggcatta atatggatat
     7561 gactgaggtt aaacagctta acgaggcatt gtttcaggaa aaagagcgcc tgcacattac
     7621 gctggactcc atcggcgaag ccgtggtctg tattgatatg gcgatgaaaa ttacctttat
     7681 gaatccagtg gcggagaaga tgagcggctg gacgcaggaa gaagcgttag gtgttccgct
     7741 cctgacggtg ttgcatatta cttttggcga caacggacca ttaatggaga acatttacag
     7801 tgccgacacc tcacgttccg cgatcgaaca agatgtggtg ttgcactgtc ggagcggcgg
     7861 cagctacgac gtgcattaca gtattacgcc gttaagtact ctggacggca gcaatatagg
     7921 ctcggtactg gtgattcagg acgttaccga atcacgcaaa atgctgcgcc agctgagcta
     7981 cagcgcctcc catgatgcac tgacgcatct cgccaaccgc gccagttttg agaaacaact
     8041 gcgtatcctg ctgcaaacgg taaacagtac gcatcagcga catgcactgg tgtttatcga
     8101 tcttgatcgc tttaaagcgg tgaatgacag cgccgggcat gcggcgggcg acgctttgct
     8161 gcgcgaactg gcgtcgttga tgctgagtat gctgcgctcc agtgacgtgc tggcgcggct
     8221 cggcggtgat gaatttggtc tgctgctgcc agactgcaat gttgaaagcg ctcgttttat
     8281 cgctacacgt attatcagtg ctgtgaatga ctatcacttt atatgggaag gccgggtaca
     8341 tcgggtaggt gccagcgccg ggattacctt gatcgatgac aacaatcatc aggcggcaga
     8401 agtgatgtcg caggctgata tcgcctgtta tgcctccaaa aatggtggcc ggggccgggt
     8461 gacggtttac gaaccgcagc aagctgccgc acatagcgaa cgggcggcga tgtcgcttga
     8521 tgaacagtgg cggatgatta aagagaatca gttgatgatg ctcgcccacg gtgtcgcttc
     8581 gccacggatc ccggaagcgc gtaatttgtg gctgatttca cttaagctct ggagttgcga
     8641 aggcgagatt attgatgaac aaacatttcg tcgtagcttc agcgatccgg cgcttagcca
     8701 tgctcttgac cgccgggtat tccacgaatt tctccagcag gccgcaaaag cggttgccag
     8761 taaaggcata agcatcgccc tccccctttc cgttgccggt ttgagtagcg ccacgctggt
     8821 gaatgatctg cttgagcagc tggaaaatag ccctctacca ccacggttat tacatctgat
     8881 tattccggca gaagcgattt tcgatcacgc agaaagcgtg caaaaactgc ggctgggggg
     8941 atgccggatc gtattcagtc aggtgggccg cgatctgcaa atcttcaact cgctgaaagc
     9001 gaatatggca gattacctgc tacttgatgg tgagttatgc gccaacgtgc agggtaattt
     9061 gatggatgag atgctgatta cgattattca ggggcacgct cagcgactcg ggatgaaaac
     9121 catcgccggg ccagtcgttt tacccttagt gatggatacg ctttctggca tcggcgtcga
     9181 tctgatttat ggtgaggtga ttgccgatgc ccaaccgctg gatttgctga tgaatagtag
     9241 ttatttcgcg attaactgag ggagcgtttg cccattgcct gatgcgacgc taacgcgtct
     9301 tatcaggcct acaaatcgct cattccgcaa ggcgctcgca ccgcatccgg cgaccaacgt
     9361 catgcttcgt ctggttgcca gccttccgta taccagatat gcaacagcgc ataagaacgc
     9421 cagggcttcc agcgctcggc ataacggcgg atttgcgccg gtgtcattcc cggaaatcgc
     9481 tgtttaatca gataatcatc cggcaggaaa acatctttcg cctgccagcc acgcaaagca
     9541 aaataattcg ccgtccagcg cccgataccc ggaaaagttt gcagcgtttt catcgcctgc
     9601 tccacatcgc ccggtattgt cattggtaag gtgccctcca gcgccgcatt tgccagatga
     9661 atcagcgcct ctgcccgttt caacggcata cctaacgctt ttaatgcctg cgggtctgct
     9721 gctgccagcc gctgaggcgt ggggaagcag atatactccg gaaaatcatc cagccgttcg
     9781 ccataaagct gtaccacttt ggcagtcaat tttgccgcca tcgccacgct caccagttgg
     9841 cctaaaatcg cccgcacgcc ttgctcaaaa gcatcaacac tgccgggtaa acgcaatccg
     9901 ggccgcgccg cgcctaactt gcccaacgca ccgttaacaa tctgcgggtt acattgcaga
     9961 tcaagcaggc ggctcatttt cgccagacac tcagcggcaa cgggttctaa atctgcactt
    10021 aaatttatgt gcagagtatg gcgggctata tccggaatag cagtcaccac gccgcgatat
    10081 tcgcccaccg ccagactacg ggcataataa tcgtcagcga ccgtttccac accactcact
    10141 gcacgggcgg cgagaaatcc caacatccac gaccagtcat acggcggctg ccagttcagg
    10201 gtatacatcg catctcctta ttcatccgct ttcagcataa acgttattca gacgctgcgc
    10261 tttgctttca tattccggtt gtcgcgacgg caacatttcg ctaaagtcac gccccttctt
    10321 caccggcatg gggattattt cgtgtttatt ggttttgatt acggtacagc aaactgttca
    10381 gtagcggtca tgcgtgacgg taaaccgcac ttgctaaaaa tggaaaacga cagcacgctg
    10441 ctgccttcaa tgctttgcgc gccaacgcgt gaagcggtaa gcgaatggct gtaccgccat
    10501 catgatgttc cggcagacga cgatgaaacg caggcactgt tgcgtcgggc gattcgttat
    10561 aatcgcgaag aagatatcga tgttacggcg aaaagcgtgc agttcggtct ttcctcactg
    10621 gcacagtaca ttgatgatcc agaagaagtg tggtttgtga aatcaccaaa atcgttcctc
    10681 ggtgccagcg gcttaaaacc gcagcaggta gcgctgtttg aggatctggt ctgcgcaatg
    10741 atgttgcaca ttcgccagca ggcgcaagcg cagctgccag aagcgattac tcaggcggtg
    10801 attggtcgcc cgatcaactt ccaggggctg ggcggcgatg aagcaaacgc ccaggcgcaa
    10861 gggattctgg aacgcgcggc gaagcgtgcc ggattcaggg acgtggtatt ccagtacgag
    10921 ccggtagcgg ctgggctgga ttacgaagcc accttacagg aagaaaaacg ggtactggtg
    10981 gtggatatcg gcggtggtac gactgactgt tcattgctgc tgatggggcc gcagtggcgt
    11041 tcgcgtctcg atcgtgaagc cagcctgctg ggtcacagtg gttgccgtat tggcggtaac
    11101 gatctggata tcgcactggc gtttaaaaac ctgatgccac tgctgggtat gggtggcgaa
    11161 accgaaaaag gcatcgccct gccgattctg ccgtggtgga atgcggttgc catcaacgac
    11221 gtacctgcgc agagtgattt ctacagtagt gccaacggtc gtctgcttaa cgatctggta
    11281 cgcgatgccc gcgaaccgga aaaagtggcc ctgctacaga aagtctggcg tcagcgttta
    11341 agctatcgcc tggtgcgtag cgcagaagag tgcaaaattg cgctttcaag cgtagcggaa
    11401 acccgcgcct cactgccgtt tatcagcgat gaactggcta cgctgattag ccagcaaggg
    11461 ctggaaagcg ccctcagcca gccactggcg cggattcagg aacaggtgca actggcgctg
    11521 gataacgccc aggaaaaacc ggacgttatc tacctgaccg gcggaagcgc ccgttctccg
    11581 ctgattaaaa aagcgctggc agaacagttg ccgggcattc cgattgcagg cggcgatgac
    11641 tttggctccg tcaccgccgg gctggcacgc tgggcggaag tggtgtttcg ttaattgaaa
    11701 aatggctcag cagcggctta aatctgcctg cgcctgagcc agtttttctg ccgcctgacg
    11761 taacggttcc atcaacggca tggtccgtgc tggagcatga aggcggaatc gctgcaactc
    11821 tgtcgcgccg acagtcaacg ccgattccag ccgatgtcgt ttggcggcca ttttcgccat
    11881 taccgcttgt ctttctgcag gcgttaccgc ttcgttcggc ctgaacacaa aacggcgttc
    11941 acagctcgct ttccagtcga taaccgcctg ggtcagatga tcaccaaacc ctttaacttg
    12001 cttaacccca cgacgggtaa catccgcggc tgtttcaata ccaaaagacc gtaacgccgc
    12061 tttacgcgca gggccaacgc cgggaataga ggcagcatca ataaaaaatc cctccagaaa
    12121 cttctgcttc tgccgttccc ttgcagtgtc atgaagtgct gccagagcgc gtttctcctc
    12181 ttccggtaac ccgagaattt cgtccttcat tttttcgagc atcgcccgtt tggcgataaa
    12241 accttccagc ccgcccaact gttggatctg gctgactaaa tggtcatagt ccattttcgc
    12301 gcgattaaat gcctcacggc gttgctggat ttctgcttta taaactttgc ttgtcagact
    12361 gccaataatc cagatagccg ccagcacagg aaccaaaata atataacgcg gttctgcctg
    12421 aaggccgcaa agcaacagcg ataacgctga cagtgcgatc tcaatcagaa tgatgtattc
    12481 gcgccgtaac aggcctaaag gaagcggcct gccagtcggt tggaaatgat cgggtaatgg
    12541 caattgcaat gctggcggtg ctactgacgc catcaccatc gcccagactt tcgccagcac
    12601 aaaatcacca ctggtggtaa tgacctcttc gccgagatca ataaaataga taacgccttg
    12661 attatccagc gcacaccacg ggcagtcagt caaatgaccg gggtaaacat gcattgccga
    12721 aacggtacat ttctttaact gttggcgtag taaatccagt gccgctaccc acgcttttgc
    12781 cgtcggacgc gcggttgcca cgccactttc cgtgaatgcc tgctgaaaca tggcttcaac
    12841 atcgcccggt aacatcgaca gcggaatcga tcgtggcggc ggttttaaac cacgtcgctg
    12901 gttgtctgac gcgtaggcat aacggaaatg ggcaatatcc gtctccagcg cattacccgc
    12961 atcagagata agcggcacac cagaataagg atgccgccca ccaaacaaga cgtgaaaaat
    13021 cagcaacgca aggccaaaat tatcgtgatt cgcggtacgt tcaaaaccga caaatgatgg
    13081 caaggtttgt agctctggcg gcgtaaaatg cgacacgccg acttcgcata aatgcagtgt
    13141 gccattggcg ttaatctgaa aggagtcact atcgatcaac accactttgc tgtcgcgacc
    13201 taccataaag ctgttctggt ttacgtcccc cacgacgtgc ccgtgctcgt gaaccgtagc
    13261 aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa tcccacgcac aatgaggata
    13321 actctggcga cgatgtgccg ggctatagat catatgaatc ggttctttac cagaaacttt
    13381 tggcatcata aaaccgataa cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca
    13441 ggcgacataa ttcaacaact gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg
    13501 ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg ctatcgacaa actcattgat
    13561 atcataaacc gcgccttcgc cacctttgcc cagttcacgg cctggcgtaa cgcactcacc
    13621 agtagcagta tataaagtgg gtttcatggt aaccacagcg ccagtgtcaa cgttttatca
    13681 tcatcggtac gctcgtttac cgctgggctc gacaaaaact gctttaacaa ttcaggtaat
    13741 aaatcgagtt gttcctgcgt tgctgccgcc agtccattga aaaacggggt aaagaaaggc
    13801 acatgcggag aattatccag catatttaac gccagtcgct gaataccatc cgtaaatgct
    13861 gcaactttat gcgcacgctc agtgctggtg aaagtttcca gtctggaaac gacatcttca
    13921 tcggtaatga aatgcgtcat attggcatat tcacccacca tcggcgtaag cggtaattgc
    13981 agaccgtgac caaaatccac caccacgccg ccatcgccaa tctgcataat caaggtgccg
    14041 ttagccgatg aaatcaatcc cagaaacgtg caggcaaaat cgcgaactgc taattcttta
    14101 gcttccgcct cggcaaaaag tcgctgacga acggtcagca caatatccgt ggcgagaata
    14161 tcattgagtc caaattcacc gtgctgcact ttttgcgaca cataagccat cgcttcattg
    14221 atggcaagca tcgcgccttc gccaccctgc gagacactgc ctgcgccatc agcaaggaac
    14281 atcaccagta atggctgctg gtcatttagc caggcaacct gcatctggca ggcatcctga
    14341 caaggtaaat cagcgctaat gtgcgacgtt ccaacagcgg atgcataaac cagacgccag
    14401 ctcacactga cgtccagcct tttggcgctt ccagcacaac ttccgttccc ggcgtggagc
    14461 gggaaaccga acgcagcgaa ctggataacc agctaaacag ttcacggaat tgtaatccct
    14521 gcagtggcaa aggctgacga acgctgattt gtgccagagt cttaatatcc gccccctgaa
    14581 caccgatgga aaagaaggca aacttcttat cttcttcccc ctgaaacact ttgttggcgg
    14641 cagcctgcca ctcatcggtt ggtgcaccat cggtgatcag gaaaatccac gggcgataat
    14701 acgaaatacc atttgcacga tactcacgtt tccgctcctc taccatatcc agggctttgg
    14761 taatggcggc acccattggt gtatccccct gggcaaacag gatgggcggg aaaaaattag
    14821 cggcgctggt aaagggttgt tccacatgca ccgggccgaa cgtcacaata ccaagttcca
    14881 ctctttttaa ggccagcggg tcggcaagca gctcatcgcg aaaggtaacc aatccggcgt
    14941 taagttcatt aatgggtcta ccgttcatcg aaccagaaac atccagcagc aaaatgcagg
    15001 ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca
    15061 taagaaatcc tttcttagag aattattgag gtccggtatc ataccgattg caataatgaa
    15121 ataagagcaa acgaagaaac tttcagaatt aggcccggcg gcgcagaaaa ttttgctatt
    15181 cgcctcgcaa gaacggagag ccgtcgattg acactaaggg cggagtgaca taatttcagg
    15241 agtgagggtt agggaaaggt ttccccctcc ccctggtgtt cttagtaagc ctggaagcta
    15301 atcactaaga gtatcaccag tatgatgacg tgcttcatca taaccctttt cttatttaag
    15361 gccccttcct cgggaggggc tttcccgttt cagcatcccg ctgaaatcgt cggcttacct
    15421 cctttcgcca tgcatgcagt ctatcgctga cttgcggata aaatagtttc cagtgtgatt
    15481 attgcgtaac gtctcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcag
    15541 attgacaatc ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc
    15601 gccctggtag tcttagtaag ccgggaagct tatgactaag agcaccacga tgatgatcca
    15661 cttcatcatg accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca
    15721 gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg aattcagtct atcgctgact
    15781 tgcggataaa atagtttcct ctgtgattat tgcgtaacgt ctcgcaaaga atatttgctg
    15841 gcaggatcgc agactgcaaa gcctgcggat tgacattctg aacgtgaagg catactttca
    15901 ggagtgaggg tagagcgggg tttcccccgc cctggtagtc ttagtatgct gggtagctaa
    15961 tcactaagag caccacgatg atgagtatct tcatcatgac cctttcctta tttatggccc
    16021 cttcctcggg agggggcttt cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct
    16081 ttcgccatgc aagcagtcta tcgctaacgc gtagataaaa tagtttcctg tgttattact
    16141 ggatgcgtgc tcgcaaatgt gcccgccatt cagacgattc ccgatagtgt ttcataattc
    16201 ctccattttt ctcccttatt ggctggctac actagtatca ttccgcgaaa cgtttcagga
    16261 agagaaactc ttaacgatga aaggcagtta taaatcccgt tgggtaatcg taatcgtggt
    16321 agttatcgcc gccatcgccg cattctggtt ctggcaaggc cgcaatgact cccagagtgc
    16381 agccccaggg gcgacgaaac aagcgcagca atcgccagcg ggtggtcgcc gtggtatgcg
    16441 ttccggccca ttagccccgg ttcaggcggc gaccgccgta gaacaggcag ttccgcgtta
    16501 cctcaccggg cttggcacca ttaccgctgc taatactgtt acggtgcgta gccgcgtgga
    16561 cggtcaactg atggcgttac atttccagga aggccagcag gtcaaagctg gcgatttact
    16621 ggcagaaatt gaccccagcc agttcaaagt tgcattagca caagcccagg gccaactggc
    16681 aaaagataaa gccacgctta ccaacgcccg ccgtgatctg gcgcgttatc agcaactggc
    16741 aaaaaccaat ctcgtctccc gccaggagct ggatgcccaa caggcgctgg tcagtgaaac
    16801 cgaaggcacc attaaggctg atgaagcaag cgtcgccagc gcgcagctgc aactcgactg
    16861 gagccgtatt accgcaccag tcgatggtcg cgttggtctc aagcaggttg atgttggtaa
    16921 ccaaatctcc agtggtgata ccaccgggat cgtggtgatc acccagacgc atcctatcga
    16981 tttgctcttt accctgccgg aaagcgatat cgctaccgtc gtgcaggcgc aaaaagccgg
    17041 aaaaccgctg gtggtagaag cctgggatcg caccaactcg aagaaattaa gtgaaggcac
    17101 gctgttaagt ctcgataacc aaatcgatgc cactaccggt acgattaaag tgaaagcacg
    17161 ctttaataat caggatgatg cgctgtttcc caatcagttt gttaacgcgc gcatgttagt
    17221 cgacaccgaa caaaacgccg tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg
    17281 ccattttgtc tgggtgctga atagcgaaaa caaggtcagc aaacatctgg tgacgccggg
    17341 cattcaggac agtcagaaag tggtgatccg cgcaggtatt tctgcgggcg atcgcgtggt
    17401 gacagacggc attgatcgcc tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag
    17461 cgccaccact ccggaagaga aagccaccag ccgcgaatac gcgaaaaaag gagctcgctc
    17521 ctgatgcaag tgttaccccc gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt
    17581 cctgtggcca ccacgctgct gatggtggcg atcttactcg ccgggattat cggttatcgc
    17641 gccctgcccg tttcggcgct gccggaagtg gactatccga ccattcaggt ggtcacgctc
    17701 tacccaggtg cgagcccgga tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag
    17761 ttcgggcaga tgtctggcct gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt
    17821 atcactttac agttccagct aacattaccg ctggatgtcg ccgagcagga agtgcaggcc
    17881 gcgattaacg ctgcgaccaa cttgttgccg agcgatctgc ctaacccgcc ggtttacagc
    17941 aaagtgaacc cggcagatcc gccgatcatg acgctcgccg tcacctcaac agccatgccg
    18001 atgacgcaag tggaagatat ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc
    18061 ggcgtcggcc tggtgacgct ttccggcggc cagcgtccgg ctgttcgcgt caaacttaac
    18121 gctcaggcga ttgccgccct cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc
    18181 gctaacgtta actcggctaa aggtagcctt gacggccctt cccgtgcggt tacgctttcc
    18241 gctaacgacc agatgcaatc cgccgaagag tatcgccagc taatcatcgc ctaccagaac
    18301 ggcgcgccga ttcgtctggg cgatgtcgca actgtagagc aaggtgcaga aaacagctgg
    18361 ctcggcgcat gggcgaataa agaacaggcc attgtgatga atgttcagcg ccagcccggt
    18421 gctaacatta tctccaccgc cgacagcatc cggcagatgc tgccacagct caccgagagt
    18481 ctgccgaaat cggtgaaggt gacggtactt tctgaccgta ccaccaatat ccgcgcatcc
    18541 gtcgatgata ctcagtttga attgatgatg gctatcgcgc tggtagtcat gattatctac
    18601 ctgtttttgc gcaatattcc ggcgaccatc attcccggcg ttgcagtgcc gctgtcgtta
    18661 atcggcacct tcgcggtcat ggtgtttctc gatttttcaa tcaataacct gacgctgatg
    18721 gcgttaacta tcgccaccgg gttcgtggtc gatgacgcca tcgtggtgat cgaaaatatt
    18781 tcccgctata tcgaaaaagg cgaaaaaccg ttggcggcgg cgctcaaagg cgcaggtgaa
    18841 atcggcttta ccattatctc gctgaccttc tcgctgattg cggtgttgat cccactgctg
    18901 tttatgggcg atatcgtcgg gcgactgttc cgcgaatttg ctattaccct ggcggtagcg
    18961 attttaatct cagccgtggt gtcgctaacc ctgacaccga tgatgtgcgc acggatgctc
    19021 agccaggagt cgttgcgtaa acagaaccgc ttctcccgtg cctcggaaaa aatgttcgac
    19081 aggataatcg ccgcctatgg tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc
    19141 ttaagcgtgg cgctcagcac gctgctgctt agcgttctat tgtgggtgtt cattccgaaa
    19201 ggtttcttcc cggtacagga taacggcatt attcagggca ctttgcaggc accgcaatcc
    19261 agctcctttg ccaatatggc ccagcggcaa cgccaggtcg cggacgtgat tttgcaggat
    19321 ccggcagtgc aaagtctgac ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaac
    19381 agtgcgcgtt tgcagatcaa cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa
    19441 gtcatcgccc gtctgcaaac ggcggtggat aaagtgccgg gcgtcgatct cttcctgcaa
    19501 ccaacgcagg acctgaccat cgatactcag gtcagccgca cccagtacca gtttaccttg
    19561 caggccacgt cactggatgc gctcagtacc tgggtgccac agttgatgga aaaactccag
    19621 cagctgccac agatttctga tgtctccagc gactggcagg acaaagggct ggtggcgtat
    19681 gtcaatgttg atcgcgacag cgccagccgt ctggggatca gcatggcgga tgtcgataac
    19741 gccctgtaca acgcgtttgg tcagcgactg atttccacta tttatactca ggctaaccag
    19801 tatcgtgtag tgctggagca caataccgaa aataccccag gcctcgcggc gctggatacc
    19861 attcgcctga ccagcagcga cggcggcgtg gtgccgctaa gctcaattgc caaaattgag
    19921 cagcgttttg cgccgctttc catcaaccat ctggatcagt tcccggtaac gaccatctcc
    19981 tttaacgtgc cggataacta ttcgctgggc gatgcggtgc aggcaattat ggacaccgag
    20041 aagacgctga atctgccggt ggatatcacc acgcaattcc agggcagcac cctcgccttc
    20101 cagtcggcgc tgggcagcac tgtctggctg attgtcgcgg cggtggtggc gatgtatatc
    20161 gtgctcggca ttctgtacga gagctttatt cacccgatca ccattctctc gacgctaccc
    20221 acggcaggtg ttggcgccct gctggcgtta atgattgccg gtagcgaact ggatgtgatt
    20281 gcgattatcg gcattatttt gctgatcggt atcgtgaaga agaacgccat catgatgatc
    20341 gacttcgcgc tggctgctga gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag
    20401 gcttgtctgt tgcgttttcg tccgatcctg atgaccaccc tggcggctct gcttggcgcg
    20461 ctgccgctga tgttgagtac cggggtcggc gcggaactac gtcgtccgtt aggtatcggc
    20521 atggtcggcg gcctgattgt cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat
    20581 ctgctgtttg accgcctggc attgtggacc aaaagtcgct ttgctcgcca tgaagaggag
    20641 gcgtaagtga agttttttgc cctcttcatt taccgcccgg tggcgacgat tttactgtcg
    20701 gtcgccatta ccctgtgcgg catactgggt ttccgtatgc tgccggtcgc cccgctgccg
    20761 caggtcgatt ttccggtgat tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca
    20821 atggcgtctt ccgttgccac gccgctggag cgctcacttg ggcgcattgc cggggtcagt
    20881 gaaatgacct ccagcagttc gctcggcagc acgcgtatta ttttgcagtt tgattttgac
    20941 cgggatatca acggtgcggc gcgtgatgtg caggcggcga tcaacgccgc acaaagtctg
    21001 ctgcccagtg ggatgcccag ccgcccgact tatcgcaaag cgaacccgtc ggatgcgcca
    21061 attatgatcc tcacgctaac ctccgatact tattcgcagg gtgaactgta cgatttcgcc
    21121 tcgacgcagc tggctccgac gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggc
    21181 ggcagctcgc tgcccgccgt gcgcgtcggg ctgaacccgc aggcgctgtt taatcagggc
    21241 gtgtcgctgg acgacgtacg cactgccatc agcaatgcca acgtgcgtaa accgcagggg
    21301 gcgctggaag atggcactca ccgctggcag atccagacca acgatgaact gaaaaccgcc
    21361 gctgaatatc agccgctgat tattcactac aacaacggcg gcgcggttcg tctgggcgat
    21421 gtggcgacgg tgaccgactc agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa
    21481 ccggctattt tactgatgat ccgcaaattg ccggaagcca atattatcca gacggtagac
    21541 agcatccggg caaaattacc ggagttgcag gaaaccattc cggcggcgat tgatctgcaa
    21601 attgcccagg atcgctcccc caccattcgc gcctcgctgg aagaagtcga gcaaacgctg
    21661 attatctcgg tggcgctggt gattctggtg gtctttttat tcctgcgctc gggtcgcgcc
    21721 actattattc ccgccgttgc ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac
    21781 ctgtgcggat tcagtctcaa taacctttcg ttaatggcgc tcaccatcgc tactggtttc
    21841 gtggtggatg acgccatcgt ggtgctggaa aacattgcac gtcatctgga agcgggaatg
    21901 aaaccgttgc aagccgcact gcaaggtact cgcgaagtcg gttttacggt gctgtcgatg
    21961 agtctgtcgc tggtggcggt gttcctgccg ctgctgttga tgggcggatt gccgggccga
    22021 ctgttacgcg agtttgccgt gacgctttct gtcgccattg gtatttcgtt gctggtttct
    22081 ctgacattaa cgccaatgat gtgtggctgg atgctgaaag ccagcaagcc gcgcgagcaa
    22141 aagcgactgc gtggttttgg tcgcatgttg gtagccctgc aacaaggcta tggtaagtca
    22201 ctgaaatggg tgctcaatca tacccgtctg gtgggcatgg tgctgcttgg caccattgct
    22261 ttgaatatct ggctatatat ctcgattccg aaaaccttct tcccggagca ggacactggc
    22321 gtgttgatgg gcgggataca ggcggatcag agtatttcgt ttcaggcgat gcgcggtaag
    22381 ttgcaggatt tcatgaaaat tatccgtgac gatccggcag tggataatgt caccggcttt
    22441 accggcggtt cgcgagtgaa cagcgggatg atgtttatca ccctcaagcc acgcgacgaa
    22501 cgcagcgaaa cagcgcagca aattattgac cgtctacgcg tgaagctggc gaaagaaccg
    22561 ggggcgaatc tgttcctgat ggcggtacag gatattcgcg ttggcggacg tcagtcgaac
    22621 gccagctacc agtacacgtt gttatccgac gacctggcgg cactgcgcga atgggagccg
    22681 aaaatccgca aaaaactggc gacattgccg gaactggcgg acgtgaactc cgatcagcag
    22741 gataacggcg cggagatgaa tctggtttac gatcgcgaca ccatggcacg gctgggaatc
    22801 gacgtacagg ccgccaacag cctgttaaat aacgccttcg gtcagcggca aatctcgacc
    22861 atttaccagc cgatgaacca gtataaagtg gtgatggaag tggatccgcg ctatacccag
    22921 gacatcagcg cgctggaaaa aatgttcgta atcaataacg aaggaaaagc gatcccgctg
    22981 tcgtatttcg ctaaatggca accggcgaat gccccactat cggtgaatca tcagggatta
    23041 tcggcagcct cgaccatctc gttcaacctg ccgaccggaa aatcgctctc ggacgccagt
    23101 gcggcgatcg atcgcgcaat gacccagctt ggtgtgcctt cgacggtgcg cggcagtttt
    23161 gccggcacgg cgcaggtgtt ccaggagacg atgaactcac aggtgatcct gattatcgcc
    23221 gccatcgcca cggtgtatat cgtgctgggt atcctttacg agagttacgt acatccgctg
    23281 acgattctct ccaccctgcc ctcggcgggc gttggagcgc tgctggcgct ggagctgttc
    23341 aatgccccgt tcagcctaat cgccctgata gggatcatgc tattaatcgg cattgtgaag
    23401 aaaaacgcca ttatgatggt cgattttgcg cttgaagccc aacggcacgg taacctgacg
    23461 ccgcaggaag ctattttcca ggcctgtctg ctgcgttttc gcccgattat gatgactacc
    23521 ctggcggcgc tgtttggcgc gctgccgctg gtgttgtcgg gcggtgacgg ctcggagctg
    23581 cggcaaccct tggggatcac cattgtcggc ggtctggtga tgagtcagct cctgacgctg
    23641 tataccacgc cggtggtata tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa
    23701 tctaaacaaa cggtaaccga gtaaatgaca gatcttcccg acagcacccg ctggcaattg
    23761 tggattgtgg ctttcggctt ctttatgcag tcgctggaca ccaccatcgt aaacaccgcc
    23821 cttccctcaa tggcgcaaag cctcggggaa agtccgttgc atatgcacat ggtcattgtc
    23881 tcttatgtgc tgaccgtggc ggtgatgctg cccgccagcg gctggctggc ggacaaagtc
    23941 ggcgtgcgca atattttctt taccgctatc gtgctgttta ctctcggttc actgttttgc
    24001 gcgctttccg gcacgctgaa cgaactgttg ctggcacgcg cgttacaggg cgttggcggt
    24061 gcgatgatgg tgccggtcgg caggttgacg gtgatgaaaa tcgtaccgcg cgagcaatat
    24121 atggcggcga tgacctttgt cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg
    24181 ctcggcggtc tgctggtgga gtacgcgtcg tggcactgga tctttttgat caacattccg
    24241 gtggggatta tcggtgcgat cgccacattg atgttaatgc cgaactacac catgcagacg
    24301 cggcgctttg atctttccgg ctttttattg ctggcggttg gcatggcggt attaaccctg
    24361 gcgctggacg gcagtaaagg tacaggttta tcgccgctgg cgattactgg cctggtcgca
    24421 gttggtgtgg tggcactggt gctttatctg ctgcacgcca gaaataacaa ccgcgctctt
    24481 ttcagtctga aactgttccg cactcgtacc ttttcgctgg gcctggcggg gagctttgcc
    24541 ggacgtatcg gtagcggcat gttgcccttt atgacgccgg ttttcctgca aattggcctt
    24601 ggcttctcac cgtttcatgc cggactgatg atgatcccga tggtgcttgg cagcatgggg
    24661 atgaagcgga ttgttgtaca ggtggtgaat cgctttggtt atcgtcgggt actggtagcg
    24721 accacgctgg gtctgtcgct ggtcaccctg ttgtttatga ctaccgctct gttgggctgg
    24781 tactacgttt tgccgttcgt cctgttttta caagggatgg tcaactcgac ccgtttctcc
    24841 tccatgaaca ccctgacgct gaaagatctc ccggacaatc tggcgagcag cggcaacagt
    24901 ctgctgtcga tgattatgca attgtcgatg agtatcggcg tcaccatcgc cgggctgttg
    24961 ctgggacttt ttggttcgca gcatgtcagc gtcgacagca gcaccacaca aaccgtcttt
    25021 atgtacacct ggcttagcat ggcgtttatc atcgcccttc cggcgttcat ctttgccaga
    25081 gtgccgaacg atacgcatca aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga
    25141 agttctggcg acctggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg
    25201 tcttgctgat cagtatgcac tgggcggtgc gtatcagttt tgagcgtggc tttattgatt
    25261 acatcaagca tggtaatgaa cagcgattac aactgttaag tgatgcgctt ggcgagcagt
    25321 atgcgcagca tggcaactgg cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc
    25381 tgcgttcatt tgaacacgat aattcggaag ataaacccgg cccgggtatg ccaccacacg
    25441 gctggcgtac tcagttctgg gtggttgatc aaaacaacaa ggtgctggtt ggtccgcgag
    25501 cgccgattcc acctgacggt acacggcgac ccattctggt caacggtgcg gaagtgggcg
    25561 cggtgattgc ctcccctgtt gagcggctga cccgcaatac tgatatcaat ttcgataaac
    25621 aacagcggca aaccagctgg ctgattgtcg ccctggcaac gttactcgcg gcactcgcta
    25681 cttttctgct ggcgcgcggt ttgctggcac cggtaaaacg acttgtcgat ggcacgcaca
    25741 aactggcggc gggcgatttc actacccgcg taacgcccac cagtgaagat gaactgggca
    25801 aactggcgca agacttcaac cagctcgcca gcacgctgga gaaaaaccag caaatgcggc
    25861 gcgattttat ggccgatatt tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg
    25921 aactggaagc cattcaggat ggcgtgcgca aattcacgcc ggagacggtg gcgtctttac
    25981 aggcggaggt cggtacactg accaaactgg ttgacgatct tcatcagttg tcgatgtctg
    26041 atgaaggcgc tctcgcctat caaaaagcac cggtagattt gatcccactg ctggaagtgg
    26101 caggcggcgc atttcgcgaa cgatttgcca gccgtggcct gaaactgcaa ttttccctgc
    26161 cagacagtat caccgtattt ggcgatcgcg accgtttaat gcagttattc aataacttac
    26221 tggaaaacag cctgcgctac actgacagcg gcggcagcct gaaaatctct gccgagcagc
    26281 acgacaaaac ggtgcgcctg acctttgccg acagtgcgcc aggtgtcagt gacgatcagc
    26341 tacaaaaatt gtttgaacgt ttttatcgca ccgaaggttc ccgcaaccgc gccagcggcg
    26401 gttccgggct ggggctggcg atttgcctga acattgttga agcacataat ggccgcatta
    26461 ttgccgccca ttcgcctttt ggcggggtaa gcattacagt agagttaccg ctggaacggg
    26521 atttacagag agaagtatga ccgagttacc aatcgacgaa aacacaccgc gtattttgat
    26581 cgtggaagat gaaccgaagc tgggacagtt gctcattgat tatctgcgtg ctgcgagcta
    26641 tgcgccgacg cttatcagcc acggcgatca ggtactgtcg tacgtgcgcc agacgccgcc
    26701 ggatctgatc ctgttggatc tgatgctccc tggcaccgat ggcctgacgc tgtgccggga
    26761 aattcgtcgt ttttctgaca ttcctatcgt gatggtgaca gcaaaaatcg aagagatcga
    26821 ccgcctgcta gggctggaga ttggcgcaga tgactacatt tgcaagccgt acagcccacg
    26881 ggaagtggta gcgcgtgtca aaaccatttt gcgccgttgc aaaccgcagc gtgagttgca
    26941 gcaacaggat gctgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcatggcg
    27001 cggtaaaatg cttgacctga cgcctgcgga atttcgtctg ctgaaaacgc tctctcatga
    27061 accaggaaaa gtgttctccc gcgagcaatt gctcaatcat ctttatgacg actaccgcgt
    27121 agtaaccgac cgtaccatcg acagccacat taaaaacctg cgccgcaagc tggaatctct
    27181 cgacgccgaa cagtcattta tccgcgccgt ttacggcgtc ggttaccgct gggaagccga
    27241 cgcctgccgc atcgtttagt tttagcgaca ttactttgtt agccggagat ggcattcaga
    27301 ccagatccgg caacattatc ccacgcatgg tcagcaaact gacattatct ctccgccctg
    27361 gcaggcatca gaaaatcgct catactttaa tcggtaaaca gcacctttag atgctgtttt
    27421 cgatacgcaa tttcaatcaa ggagtcatta tggctggttg gtttgaactc agtaagagca
    27481 gtgatagtca gttccggttt gtgctaaaag cgggcaatgg tgagacgatc ctcaccagcg
    27541 agctgtatac ctcaaaagcc tctgcggaaa agggcatcgc gtcggtgcgt agcaacagcc
    27601 cgcaagaaga acgctatgag aaaaaaacgg caagcaacgg caaattctat ttcaatctga
    27661 aagccgccaa tcatcaaatt atcggctcca gccagatgta tgccaccgcg caatctcgtg
    27721 aaaccggaat tgcctccgtt aaagccaatg gcacaagcca gacggtgaaa gacaatacgt
    27781 aattacgatg ccgggcgtgg gttgttggcg tccggcgctt taccagatcc cccctacagc
    27841 gctacaatgc ccgcccttaa agtgggggca ctcccctaac cgcttcatca ggtgaagcgg
    27901 atctgacctg tcatcagaac gagagaatta tgtttaaacc ggaactcctt tccccggcgg
    27961 gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc agatgctgtt tatgcgggcc
    28021 agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa ccacgaaaac cttcagctcg
    28081 gcatcaatga agcccacgcg ctggggaaaa agttttatgt cgtggtcaac attgcaccgc
    28141 acaacgccaa gctgaaaacc tttatccgtg acctgaaacc ggtggtggaa atggggccgg
    28201 atgcgctgat tatgtccgat ccagggctga ttatgctggt gcgtgagcac ttccctgaaa
    28261 tgccgatcca tctctcggta caggctaacg ccgtgaactg ggcgacggtg aaattctggc
    28321 agcaaatggg actgacccgc gtgatcctct ctcgcgagct gtcgctggaa gagattgaag
    28381 agatccgcaa tcaggtgccg gatatggaga tcgagatctt cgttcacggc gcactgtgca
    28441 tggcctactc cggtcgctgc ctgctctctg gctatatcaa caagcgcgat ccgaaccagg
    28501 gtacctgcac caacgcctgc cgctgggagt acaacgtcca ggaagggaaa gaagatgacg
    28561 ttggcaacat cgtacacaag tacgagccga ttccggtgca aaatgttgag ccgacgctgg
    28621 gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga agcccagcgt ccgggcgagt
    28681 atatgaccgc gtttgaagat gagcacggca cttacatcat gaactcgaaa gatctgcgcg
    28741 ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca ttcgctgaaa atcgaaggcc
    28801 gtactaaatc tttctactat tgcgcacgca ccgcacaggt ttatcgtaaa gctatcgatg
    28861 atgccgctgc gggcaaaccg ttcgatacca gcctgctgga aactctggaa ggtctggcgc
    28921 atcgtggcta taccgaaggt ttcctgcgtc gtcatactca cgacgattat cagaactacg
    28981 aatacggtta ttcagtttct gaccgccagc agtttgttgg tgagtttacc ggtgagcgca
    29041 agggggacct cgcggcggta gcggtgaaaa ataaattctc cgttggcgac agccttgagc
    29101 tgatgacgcc gcaaggcaac attaacttta cccttgagca catggaaaac gccaaaggcg
    29161 aagctatgcc ggtagcacca ggcgatggtt atactgtgtg gctcccggtg ccgcaggatc
    29221 ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg ggaaactacg cgtaaccccc
    29281 acggtaagtg attaatttcg attatttttc ccggatggaa aattcttaga aaccgatcac
    29341 atacagctgc atttattaag gttatcatcc gtttcgctga aaaacataac ccataaaatg
    29401 ctagctgtac caggaaccac ctccttagcc tgtgtaatct cccttacacg ggcttatttt
    29461 ttatgcataa gccctatccc tgataaccgt cttccattga ccacatcgat agaatctccc
    29521 ttcatagcac gatgcctttc acgtaacggc atcgtgctcg cacaggttcc ggctaagcac
    29581 aaccagaacg cgcatgtttg acgcttacca aaaaatattc tcactctcca catttgaatg
    29641 tcagacgagc gacgccatgt aatcctgcac cttctgtctt caggtcaact atctgcattt
    29701 ttttgccctg agtaacacag aaatgggctg catcattttt tactatattt tctgcaccag
    29761 atattctgcc cctggctaaa gaagcttcgg cttcggtgta gtattggtta tcgagtttac
    29821 gctgaatatt acttttatat gcaagaccaa atttaccgat acttgtctca tcattatgca
    29881 cagcacaacc agacataaga aaaacactaa ttaatgatat agcagctatc tttttcatct
    29941 caccttcccc cattaaatac caacgacact ctctagtgtt taaatataat aatggcatga
    30001 ttattataat tgaataggat tataataaat gttctgtaaa acatttccta cataagtagg
    30061 aattacggac atggaggccc ttccgggtaa ctccatggag gctttaatat attatattga
    30121 agaagccact gtttagttga atattaggta tatgctcttt tttgaaattt atcggtggct
    30181 gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg taaccactga atcctccata
    30241 cttgaactga ctatttatcc tccgactttc atcctgttct gactccacct tttcttttct
    30301 gctctacact atctatagac caatcataaa ggcacatacg atcatggcag aatttcccgc
    30361 cagcttactg attcttaatg gcaaaagtac tgacaatcta cccttgcgcg aagcaattat
    30421 gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc acctgggaga aaggcgatgc
    30481 cgcacgatat gtagaggagg cccggaagtt gggcgtcgca acggtgattg ccggtggtgg
    30541 cgatggcacc attaatgaag tttctacggc gttgattcag tgtgaggggg atgacatacc
    30601 cgcgctggga attttgccat taggaaccgc caatgatttt gccaccagtg tagggattcc
    30661 tgaggcactg gataaggcgc tgaaactggc aattgccggt aacgccattg cgatagatat
    30721 ggcgcaggtc aacaaacaaa cctgttttat taatatggcg acaggcggat ttgggacgcg
    30781 tattaccaca gaaacgccgg aaaaattaaa agccgcgctg ggtggcgtct cttacatcat
    30841 tcatggctta atgcgcatgg atactctgca accggaccgt tgtgaaatcc gcggtgaaaa
    30901 ctttcactgg caaggtgacg ccctggtcat tggtattggt aacgggcgtc aggccggtgg
    30961 cggtcaacaa ttgtgcccga acgcgttaat taacgatggc ttgctgcaac tgcgcatttt
    31021 taccggcgat gaaattcttc cggctctcgt atcaacctta aaatctgacg aagataaccc
    31081 gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa gccccacacg aaatcacttt
    31141 taatcttgat ggcgaaccgt tgagtgggca aaacttccat attgaaatac ttccggcggc
    31201 gttgcgttgt cgattaccac cagattgtcc attattgcgt taatcaaact attctctctt
    31261 gaaccaggga gaatccgtaa tgccggatgc ggtgtaaaca ccttatccgg catacagaac
    31321 aatactacac cgtaattaat tttacccgtg tcttctgcaa tgccagttta tccacatctg
    31381 acacggcatc atcggtaata ataatgtcaa ttttttcgat tggcagtacc tgattaaaac
    31441 cgcgacgatt aaatttcgat gaatcgagaa cggcaatcac tttatttgcg gcggtgacca
    31501 tcgccccgct aatcgaataa ccttcattaa aggtcgtaat accattaacc gcatcaatac
    31561 catcagcacc gacaaacatt aaatcggcat taatatcctg caatgaacgc tcggcaatgg
    31621 aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc acagacgacc agagtaatgt
    31681 ctttattttc ggaaagggca aacgctgccg ggagactgtt ggtaatcacc gtgatatttt
    31741 tggcggtcat taatccttcg gcgataagca ttgtcgtact tccgctgtcg agaataacgg
    31801 tcatcccttc gtggatcatt ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg
    31861 caagctgaaa tcgctcttta aacccgactt cctgggtctc ggtttcacta ttaccagaca
    31921 ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca
    31981 aatcggcacg gattgtcgct tccgaggcag caaatactcc cgccagatcc tgaacaagca
    32041 cggttccctg ttcattcact aattggatga tcttattcct tcgctcgaat gaattcatcg
    32101 tcatttaccc atattcaatt gtggctagtg taaacgaagg gggaccattg gtgaatgatc
    32161 tgttttgcac aataccgggc cagcatcacg ctggcccgcg gtttcaggga atgagcaaca
    32221 ctttgcccgg catagcatta cgagcgatgt cacgcaccgc ctgggcgaag ctttcaaagc
    32281 ttccacggtg agcgattaat ggctccaggc ttaacttacg ttctgtcagc aaccggctcg
    32341 ccgtttccca ctcctgcccc ggccaagggc tggagtagtt catccaactg ccgataaccg
    32401 tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca
    32461 acgtgcccac cagtgccagt tgggcatgag gaccggcaat ctctaccgcc agttcgacag
    32521 tttgcggtac gccagccgtc tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc
    32581 tctgcatttg cggcgcgctc atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt
    32641 ttgccagtgc cagtttttct gagctaatgt cgatagccgt cacactcttt gctcccagcg
    32701 cgacagcgca ctgaatagcc agcaggccaa tggttccggc accaataata ataacgtttt
    32761 tattctcaca accttgcgct aaatgaaaag catgcaggcc aacggtaatc ggctcaataa
    32821 aagccccatc ctcaataggc atatccgtgg gtagagcaaa gacatttttt cgcttaacga
    32881 caatatattc ggcaaatcca ccatcacgcc gtgagccaat aaaatcatat tttgcgcact
    32941 gggaataaaa ccctttcaaa cactctggac aagtaaaaca gggtaataac ggcacacagg
    33001 caaccgcatc gccagggtgt aaatcatcaa caccggatcc taccgcatca atatagccgc
    33061 taaattcatg gcctaacgtt attggataat aatgtgcacc atttttaaat atcctgggta
    33121 aatcggaacc acataagccc gagctggcaa tttttacccg cacctcatcc tgatgtttaa
    33181 tttcaggaat gacgctttct gcaacgcgca cgataccatc agtatcattc accactgatt
    33241 tcataaaaac tcctaattgt taagggggat aacctccccc tcaggtaaaa attattctgc
    33301 gagaacgact ttctcttgtt taataaagcc acgcgctcta cgccaggtca tgaaaatacc
    33361 ggtaagataa attgcgccga taataatgaa accgggaata ttttgcgggg agaaaacctg
    33421 aatcagtaac caggtaatgg gagaaccgcc ctgatccatt gaagccacca tacccccggc
    33481 ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa ccaatagttt gcgtcgcgat
    33541 ccacagggtg atgctcataa tgatgacacc cgagattaag gtgcggaaca gatttccacg
    33601 atgcaccgcg accgccatcg ccacgaagaa gccgatagtg gcaagatcgc caaacggcag
    33661 tacctgatta ccaggaacac aaacagcaat taaaatggtg agcggaataa aaatcaggct
    33721 tgccgatacc accgccgtat gccccagcag taatgcggga tccaggccaa tcaggaactc
    33781 ctgaccgccg aacttcgcct gtaaacgact acgggcctgc ttagcgatgg gcgttaaacc
    33841 atccatgatg ggtttaatca cccgtggcat tagcagcatc accgccgccg tttttaccgc
    33901 cagctgcaat acacctttga catcgtaacc cgcgaggatg ccgataatca gccccatcac
    33961 aaaacccacg gtgacaggct cgccaaacgg gccaaatttg cgctgaatat cgtcggcgct
    34021 aaatttaata cggttaacgc ctgggatttt ctcgatgata gcatcgacca gcaccgcaat
    34081 cggccccata tacgccgacg taccgtgcgg aatagcaatg ccttccagct caaagaaatt
    34141 tcgggtatcg cgggcaaacc agtcgccgag cttataaaca aacgccgcgt gaattaccac
    34201 acctgccatc cctatcatcc atgaaccggt tgccagatgc agcaacgcgc cggtgaaggt
    34261 catatgccag atattccaga tatcaacatt taccacccgc gtcatacggg tcagtaacat
    34321 cgccacgtta accagaatcg caatcggaat cgccaccagc gcaatttgcg acgcccaggt
    34381 cattggtgaa gagcccggcc agccgacatc gaccacatgc agattcaggt cgaaattttc
    34441 cgccatcgct ttcgccgccg ggccaatgga atccagcatt aagccaatca caaggccaat
    34501 gccaacaaag ccaatcccga tatgcagacc cgctttaaag caatcgcctg ccttcatgcc
    34561 taaaatttta gaaaaaataa tgatgacaat cggcaacatc accgtagggc cgagatcgag
    34621 aatataacgc atgacttctg aaaacatagg tcacccctgt aagatagtca gaattttatt
    34681 ttgtaatgct tcgataccga caccagaaac aaaaggcatg ccgtgaacta acggaatatc
    34741 gccaaaacta cgatccaccc tggcagtggt gcatatcaaa tgcacgccat ccatataggt
    34801 ttctatttca ttaacccgac actggattaa ttcaacagga atattatgac tctgacacaa
    34861 ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc gcgcctccgc aagcgacaat
    34921 aatcttgcgt ttcataaata gtccttttta ttgttacaga gggatttatt cgtctgaatc
    34981 taaaacatac tttgtgaagt attcctttaa ctgagtttca ggaagagtga ttagtgtctc
    35041 aacgatatcg ggctgttgta acttgccaaa taaacagcgt aaaagtttca attgctgctg
    35101 cggattttcc acaatcaacg caataaccaa cgataccgcc acgtcattat catcatccgc
    35161 ttgctgaaaa tgaactttat ttgttggcct taacagataa atggctgacg acttagcatg
    35221 aatcgcctca caatgcggta ttgcaatggc gtgctgctca agcattatcc cggtagggaa
    35281 ttctgcttct ctggcaatta atgcctgtgg ccaggtatca tgaaccacac ctttggcgag
    35341 catctcatta ccgatatggg ttaaaacttc gctacgatcg acaaaagaaa ttccgctacg
    35401 aacaaacagg ttagtcatat accgtcctta ttccgcacag ccgtagcgat aggcgcgcaa
    35461 aacatcataa attttatcca taatcagctg atgcggtatt gctgataatt ccccggactg
    35521 aatgcgttca aattgtttgg gaagatactg actaatcatg cccagtggga tgtccacgcc
    35581 ttgcagattc accatcatcg tttcaacgct atttttaatc cgactatgcg gccagtaata
    35641 acgaatacga tccgacaggc tgtaacgaat atccagtaat gaatcgttaa aacccgtgcg
    35701 ataatatttt ttccagtatt gcggttcgtc gagcatcact tcttcaatta ccgccaggca
    35761 accgctgcga ttttcggggg cgataagttc ctgctcaatt tgcgccagcg caaatatcgc
    35821 ctcacgtaaa gcaaaggtta atgcgggacc gactttcaat attgcaaagt gatcgcggac
    35881 taattcccaa taagccgtcc gggtctggta atcggtagaa tgtgcttcat aaaccattcg
    35941 ggtattttct atccattgcg ccagcgcctg cgcttcctgc ggctgataat ggataatatt
    36001 gctgtgatca aattccacac ccggctgcac cacgatggca atcacgcgtg ttaatgcctc
    36061 tgtcagccca cgggcaataa aggccttttg atgcgtacgt aaagtattgg cggcatcttc
    36121 aacatgggtg atgtgtactg actgaatggc gctggcctca ccgcccggaa ccggaacttc
    36181 ggtgccaatg acatagctca gttgctcacg ctggcaatct gtcgccacac tttccgcagc
    36241 aaagcaaagc acagcagctc gttccgcaac cgtttctggt gctaacggta tgggatcccc
    36301 cgcgcaggac attgacgcat caagatgaat tttactgaag ccagcacgaa catatgcctt
    36361 taccagctcg acggattttt ccatcgccgc atccacattt tcttgctgcc agcagtttgg
    36421 ccccagatgg tcaccgccga gaataatacg ctcgcgtgca aatccgactt tatcggcaat
    36481 cgcaaaaaca aattcgcgaa agtctgccgg tgtcattccg gtataaccgc caaattgatt
    36541 gacctggttt gacgttgctt caatcagcac tttgcgcgtg ctgttgcgat caaatgccag
    36601 cgccgcttcg ataaccaacg gatgggcaga acagactgaa catatgccga tatgttcacc
    36661 agctttatgc cgggcaatta acgttttcat gttttttcct tgttaaatgg caggtgcgtt
    36721 atgccctgcc ctcgcagcca caatcggcaa tcactttgct caccacatcg cgcattgcgg
    36781 atttagccga ctgcaaataa tcccggggat cggtcgcttc ggggtgctcg gtcaggtaat
    36841 tttttaacgc ctgcgagaag gcatttttca gctccgttgc aacgttgatt ttgcatatcc
    36901 ccagtttgat ggtttgctga atatccttag tcgataaccc tgacgcgcca tgcagcacca
    36961 gcggtaagtt cacccactgg cgaatgttct ccagtcgaga aaaatcaagc gccggtgcgc
    37021 tggcatacat accatgagcc gtgccgatgg cgaccgccag ggaatcaatt ccggttgcct
    37081 cggcaaattc acgcgcctga gcggggttgg tataaaaagc atcggcttca ttgacttgca
    37141 catcatcttc ctggccgcca agttgcccca gctccgcttc gacgctgaca tcaaagcgat
    37201 ggcaaaaatc caccacctct ttgacccgtg aaatattttg cgcaaaaggc aaatgcgagg
    37261 cgtcaatcat gactgagcgc acgccagaac gaaccttctg agcgatatcg tcaaatttcg
    37321 tgtgatggtc gagatgaatc gccagtggat ggtgataatg cttcgccatc gcgctgacca
    37381 gcgccaacag attttctgta ccagcatgag taaatgtgcc aggcgttccg gcgatgatga
    37441 ccggcgcatg caggttggca gcggtttcta ccaccacttg catcgtttcg agattgtgaa
    37501 tattgaatgc cggaaccgcg taaccgccgc gctgcgcgtt gttcagcatc tgctttgtcg
    37561 ataccacgta cattttcaca tcctgtcgat tgctttcgat ttcaaaacat aatgaaatca
    37621 tttgttttta aatgtcgaga taacgatcac aaaaacgaca atatgaaaat taatagagga
    37681 atgaaaggca aaaaaaacgg cctcccggag ggaagccgca acaaagtgcg cgtggtttta
    37741 tgcaggatgc ggcgtaaaca tatgtggcag cgctgcgtag gcatgataag acgcttcagc
    37801 gtcgcatcag gcataggttg ccggatgcgg cgtaaacgcc ttatccgtcc tacggggtag
    37861 tgccgttcgt atcaggcgat agtaattttg ctatcgagat aaacgtcctg cacggcgtta
    37921 atcagtttca cgccgtcagc catcgatttc ttgaacgcct tacgtccaag aatcagcccc
    37981 attccgccag cgcgtttgtt gataaccgca gtacgcactg catcactgag gtcagtttca
    38041 ccgcctgcag caccaccgga gtttatcaac ccggcccggc ccatatagca gttagctaac
    38101 tgataacgca ccagatcaat cgggttttcg ctggttaact tgctgtacac gcgatcgtcg
    38161 gtataaccgt aattaattgc tttatagccg ccgttatttt ccgccatttt ttgtttgacg
    38221 atatctgcac ctatggttgc cgccagatgg ttcgcctgac cagtcaggtc ggcggagaca
    38281 tggtaatcaa cgccatcttt cttaaaggcg gagttacgca aataggccca cagcactgtc
    38341 accatgccca gctcgtgcgc acgttcaaaa gccgcagaaa tttcttcaat ctggcgacgt
    38401 gactcttccg agccaaaata gatagtcgca ccaaccgcca ccgcgcccat gttgaaggcc
    38461 tgctccacgc tggcatacag cgtttgatcg taggtattcg ggtaacttag cgtctcgttg
    38521 tgattaagtt ttacgagaaa tggaatgcga tgcgcatagc gccgcgatac cgacgccaac
    38581 acgccgtaag ttgatgccac acagttacag cccgcttcga tcgccagttc aacaatgttt
    38641 ttcgggtcaa agtagagcgg gttagcagca aatgaagctc cggcagagtg ctcaacgccc
    38701 tggtcaaccg gcagaataga aagatatcct gtgccagcca gacgcccggt gttgtacaac
    38761 gtctgcatat tacgtaacac cgctggcggg cgattattgt caatcatcac gcggtctacg
    38821 tagtcatgtc cggggagata aagctggtca gaaggaatag tcatacaacg gtgctgtaaa
    38881 aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg tcattactat gctcccgtaa
    38941 attccgattg gatatcggct atggattgtc ctggcccgcc ttttgcgggc aatcataatc
    39001 ctggtcgtta cgggcgagtt tttccatctt ttggatgttt tttcagcgtt ttctgttggc
    39061 tcgattcatc agaaaaaatg ttagcgcggt caagttttca ccgcaaaggt atttaaaagg
    39121 tattattaag tggtattgtc atcgcgtacc ttacattacc tgtcatgaag gaattaaaag
    39181 atgaaaacaa caacaaagct gtcgttcatg atgtttgttg aatggtttat ctggggcgcg
    39241 tggtttgtgc cattgtggct gtggttaagt aaaagcggtt ttagtgccgg agaaattggc
    39301 tggtcgtatg cctgcacagc cattgcggcg atcctgtcgc caattctggt tggctccatc
    39361 actgaccgct ttttctcggc gcagaaagtg ctggcggtat tgatgttcac tggtgcggcg
    39421 ctgatgtatt tcgctgcgca acagaccact tttgccgggt tcttcccgtt actgctggcc
    39481 tactcgctaa cctatatgcc gaccattgcg ctgactaaca gcatcgcttt tgccaacgtg
    39541 ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg ctggattgcc
    39601 tctggtctgg catgtggttt cttgccgcaa atgctggggt atgccgatat ctcaccgact
    39661 aacatcccgc tgctgattac cgccggaagt tctgctctgc tcggtgtgtt tgcgtttttc
    39721 ctgcccgaca cgccgccaaa aagcaccggc aaaatggaca ttaaagtcat gctcggcctg
    39781 gatgcgctga tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg ttcattcctg
    39841 tttgcgatgc cactggcgtt ctattacatc tttgccaacg gttatctgac cgaagttggc
    39901 atgaaaaacg ccaccggctg gatgacgctc ggccagttct ctgaaatatt ctttatgctg
    39961 gcattgccgt ttttcaccaa acgctttggt atcaaaaagg tattattgct tggtctggtc
    40021 accgctgcga tccgctatgg cttctttatt tacggtagtg cggatgaata tttcacctac
    40081 gcgttactgt tcctcggcat tttgctgcac ggcgtaagtt acgattttta ctacgttacc
    40141 gcttacatct atgtcgataa aaaagccccc gtgcatatgc gtaccgctgc gcagggactg
    40201 atcacgctct gctgccaggg cttcggcagt ttgctcggct atcgtcttgg cggtgtgatg
    40261 atggaaaaga tgttcgctta tcaggaaccg gtaaacggac tgactttcaa ctggtccggg
    40321 atgtggactt ttggcgcggt gatgattgcc attatcgccg tgctgttcat gatctttttc
    40381 cgcgaatccg acaacgaaat tacggctatc aaggtcgatg atcgcgatat tgcgttgaca
    40441 caaggggaag ttaaatgaaa acagaacgta ttctcggtgc tctttatggg caggcgttag
    40501 gggatgcgat ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa gcacactttg
    40561 gctggattga ccgttttctg cctggaccaa aggagaacaa cgcggcctgt tattttaacc
    40621 gcgccgaatt caccgacgat acctcgatgg cgctgtgtct ggcggatgcg ttactggaac
    40681 gtgaaggcaa gatcgacccg aatctgattg ggcgtaatat tctcgactgg gcgctgcgtt
    40741 tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa gattgcgctt aacgccattc
    40801 gcgacggtaa acccgttgct gaactggaaa acaacggcgt gaccaacggc gcagcgatgc
    40861 gcgtctcgcc attaggttgt ttgcttccgg cgcgtgatgt tgattccttt attaatgatg
    40921 tggcgctggc gtcgagcccg acccataaat ccgatctggc ggttgcgggc gcggtagtca
    40981 tcgcatgggc gatttctcgt gctattgacg gagaaagctg gtcagcgatt gttgattcat
    41041 tgccttcaat tgcgcgacat gcacagcaaa aacgcaccac cacgttcagc gcctcactgg
    41101 cagcgcggct ggagattgcg ctgaaaattg tgcgcaatgc cgacggcact gaatccgcca
    41161 gcgaacagct ttaccaggtc gttggcgcag gtaccagcac tattgagtcc gttccgtgcg
    41221 ccattgcgct ggttgaactg gcacaaaccg acccgaatcg ctgcgccgtc ctgtgcgcta
    41281 accttggcgg cgatacagac accatcggtg ctatggcgac ggcaatctgc ggcgcgttgc
    41341 atggcattaa cgctatcgat cctgcgttaa aggcggagtt ggatgcggta aatcagcttg
    41401 atttcaaccg ctatgccaca gcgctggcga aatatcgtca acaacgggag gcggcatgag
    41461 cggcgctcga ttacacacgc tgctgcctga attaaccacg cgtcagccgg ttatggtgat
    41521 tggcgcggca gtcattgatg tgatcgccga cgcttatgcc ctcccctggc gtgggtgcga
    41581 tatcgaactg aaacagcaga gcgttaacgt tggcggctgc gcactgaata ttgccgtggc
    41641 gttaaagcgc ctcggcatcg aagcgggtaa cgccttgccg ctcggtcagg gcgtgtgggc
    41701 ggagattatt cgcaaccgga tggcaaaaga gggcttaatc agcctgatcg ataacgccga
    41761 aggtgataac ggctggtgtc tggcgctggt tgagccggat ggcgaacgca cttttatgtc
    41821 attcagcggt gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa ccgttgcgcc
    41881 tggcagcctg ctctattttt ccggttatca actggcctcg ccctgcggcg aattattagt
    41941 ggaatggctg gaaaagctgc aagacgtgac gccgtttatc gattttggcc cacgtattgg
    42001 cgatatcccg gaggcattac tggcgcggat catggcctgt cgacctttag tgtcgctcaa
    42061 tcgtcaggag gctgagattg ccgccgaacg ttttgcttta tccgcagaga taacaacact
    42121 tggcaagcaa tggcaggaga aatttgccac gccgttgatc attcgtctcg ataaagaggg
    42181 cgcatggtat ttcagcaacg acgcttctgg ctgcattccg gcatttccca cgcaagttgt
    42241 agacaccatt ggggcgggcg acagtcatgc aggtggcgta cttgccgggt tggcctctgg
    42301 tctgccactg gcggatgccg tattactggg caatgcagtg gcgtcgtggg ttgtcgggca
    42361 tcggggcggt gattgtgcgc caacgcgcga ggaactactc ctcgcacaca aaaacgtata
    42421 gatcgctgcg acagtggcta atgctgtact caataggccg ttgctgttgg tcaagcgcca
    42481 cttgcttgat caccagcacc ggtattttgc tatctaactg aatatgtgac tgaaactcgg
    42541 catctggcat ccgggcgcta acgcgggaac gtgtacgttg tgggtaaatg tgctggctgc
    42601 ggaaatagtc ataaagagaa attccgatgg catcaacatc atgaattaaa tgcgccggaa
    42661 cccacgattc ctcaatcgat accgcttctt catcgacata gcgaatacgc ttaagcaaaa
    42721 agacatcact tcccgcttcg accgccagct gctgcgcgac ttcttcggga catttcacga
    42781 cacgcttgtt aacccatagc gtatcgggct ttttcccgcg caataccacc tgctgagaaa
    42841 aacctcgcgc ttctttcaac gagtattcga agatgttgtt gatctgcgtg ccgtaaccgc
    42901 gcgaacgcgt caccacaccc tcttcttcca gcgcctgcat cgccttgcgc accgtaatgc
    42961 gtgaaacgcc ggttaactga cttaaatcac gctcaccggg caaaatattg ccatgctcca
    43021 gcaccccgct gcgcacggca ttttttaccg tttcggcaaa cttaatataa agcggcgtgt
    43081 tatccgccgc taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc gcttgttcca
    43141 tttgtctttt tcctgccagg gagtctggtg ggagtatatc actgcttctt atttaaggcg
    43201 tttctttaat cccatcaacg aatgcctgca actcctcctc tgtgccatta aacacattaa
    43261 aatccaccga accattaatg ccatctacct gcccacggtc ggaatgctgc cagaaacgcc
    43321 aggccatgcc gtcattgtcc gggcgacgtt gatagtagtg agccacccac cacggatatt
    43381 cattgaaata gcccgccaga ttggtgtgat aaaaaacggc tcctgagtaa ataaccggct
    43441 tttttcccgt acttttttcg accattttta gccactgact tacccgcttg cgtaattctt
    43501 ttgccgataa ttttccccgt tcttctacgt ccagcacggc agggaaatcg ccttgtgaga
    43561 aatccaccgt ttgcagaaat aatcgcgcct gaacaggcgc tgctaccgac ggggagaaat
    43621 aatgatacgc cccgcgcagc aggccatttt cgcggcttag ttgccagtta cgcgaaaaat
    43681 aggggtccac cagcttttcg ccttccgtcg ccttgataaa ggcaaattgt aagcggatac
    43741 cgttatcgcg catttttgcc acccgctgcc agtcgatccg ctcctgccag cgcgaaacat
    43801 ctattccgtg aatggtgtaa ctggcaggaa tatgaatagc gaaagattta accgggcgat
    43861 aaccgtagaa gtggatgtag tctgtaatct gaactgctgt cgaatagaaa aaattcaccg
    43921 tctgacgcgg ataaattgca acgatggaaa ttagcccaag agcgcataat agagcggtga
    43981 gtttttttcg actggtaatt cttagctgca ttggtttcat ccctgaatgt cagtgccaaa
    44041 ggctgacaat aaccaaagca gctatagtac ggtgctcacc accaggcgtg gaagtggtga
    44101 accggaccaa taccgtgacc aacttccagc gtgtcggcct gggctaacgc cgatgaaagc
    44161 cagctttttg cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg tagtgcagcc
    44221 aacgccgcag agagtgtaca accagtgccg tgggtgtttt tggtcataat gcgcggtgcg
    44281 gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg ggctttgctc atcatccaga
    44341 tggccacctt tcattagcac tgcgccacag cccatcgcca acagcgctcg cccttgttcc
    44401 agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca aggcagcagc ttcgggcaag
    44461 tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc gtagcgtggc aactgccgaa
    44521 ggtgaaagca gtgggtcgcc actttttgcc agcataacgg tgtcgagtac cacgttttgg
    44581 atctgataac gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc cgccaacata
    44641 ccgattttag tggtatcgat tcgcacatcg ctgaacaccg aatcgagctg ggcggcgaca
    44701 aaatcaggct caatgcgata caccgactgt acgccacggg tattttgcgc caccagtgca
    44761 gtaataactg agcaaccata agcgccaagt gccgagaagg ttttaagatc ggcctgaatc
    44821 cccgcaccac cactcggatc agtaccggca atcgtcagag cgttaattcg tttcatgcct
    44881 gcacctcctg cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa ctgcctggcc
    44941 cctcgcttct ggcgactgcg cgttctccgg cttgtttcat ccagtgacag gcagatgcga
    45001 cattttccag catatcgcct ggtaacgcac aacaggcagc gacaaccgcc gataatgcac
    45061 agccagttcc taccactttg gtcattaacg gatcgccacc gtgaatgcca acggcgcgat
    45121 ggccatcggt aacataatcc acctcgccag tgaccacgac gattgcgcca gtttcccgtg
    45181 ccagtgtttg tgcagcgggt atcgcgttaa ctgcggcgtc agtggtatcc actccccgtc
    45241 caccattagc aacgccagct aatgccatga tttccgaagc attaccacgt atcgctgccg
    45301 gtttaaaaga taaaagttca tgacaaaaat ggcggcgata atcgagcgca ccaaccgcta
    45361 ctggatcaag cgtccagggt gtttgagagc tttttgcttg ctcaacggca gcacgcatcg
    45421 cctgtgcgcg tggctgcgtc agtgtgccaa cgttaatcaa caaggcactg gcgatagccg
    45481 caaactgact ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg agcgccagca
    45541 aggtattggc agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca agaggggaat
    45601 gttggtgaaa aaggtgtaac gtgtgcgcag attgcgcgga actcagcagg tcgacttgca
    45661 tagtttgctc ctgccataac gtgaagaagc aatgacctgg tggtccgtga cttccctacg
    45721 ctggcattat ccagatcagg tgatacgggt atttctcagc cttcacgcag aagggcaccc
    45781 cgagtcgttt ggttgaatct ctatattttg tgccagctta ggggaagagg cttgcagatg
    45841 caagctcgcc tccattcatt acgtcttcac tgtgtacccc atatcagtaa tgaaaaagaa
    45901 acaattcaca cccatcattc aggtggtaaa aactgtagac tatcttaatt agctaattta
    45961 tattatttat ctaaatttga taggtgagat aaaaatgaca gaaacccaga tctacgagaa
    46021 cattaagcaa gccattagca gcgcaccacg aaatagccaa acgatggaaa tgcacctcca
    46081 gatgatcaaa tacgctgacc atctgaaaaa cgtcacggca aaagagtttt gcgaaggtgt
    46141 aggacttaaa gccagctttg ctacagagtt cagcaaaatg agaaatctta ccgagcgact
    46201 caaagccgcc ggacttgata ccacgaagtt ataacaaacg aaatcatcaa tatattactt
    46261 tcgttttcca gggtt
//
LOCUS       DAZFKY010000014        46139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFKY010000014 DAZFKY010000000
VERSION     DAZFKY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(141..317)
                     /locus_tag="ACHKCL_000718"
     CDS             complement(141..317)
                     /locus_tag="ACHKCL_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761757.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            620..1246
                     /locus_tag="ACHKCL_000719"
     CDS             620..1246
                     /locus_tag="ACHKCL_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5761758.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1597..1764
                     /locus_tag="ACHKCL_000720"
     CDS             1597..1764
                     /locus_tag="ACHKCL_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5761759.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1844..3091)
                     /locus_tag="ACHKCL_000721"
     CDS             complement(1844..3091)
                     /locus_tag="ACHKCL_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO5761760.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3163..4077)
                     /locus_tag="ACHKCL_000722"
     CDS             complement(3163..4077)
                     /locus_tag="ACHKCL_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5761761.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4293..5726
                     /gene="cscA"
                     /locus_tag="ACHKCL_000723"
     CDS             4293..5726
                     /gene="cscA"
                     /locus_tag="ACHKCL_000723"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5761762.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5734..6729)
                     /locus_tag="ACHKCL_000724"
     CDS             complement(5734..6729)
                     /locus_tag="ACHKCL_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5761763.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6972..7190
                     /locus_tag="ACHKCL_000725"
                     /pseudo
     CDS             6972..7190
                     /locus_tag="ACHKCL_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7208..8536
                     /gene="dsdA"
                     /locus_tag="ACHKCL_000726"
     CDS             7208..8536
                     /gene="dsdA"
                     /locus_tag="ACHKCL_000726"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5761764.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8644..10182)
                     /gene="emrY"
                     /locus_tag="ACHKCL_000727"
     CDS             complement(8644..10182)
                     /gene="emrY"
                     /locus_tag="ACHKCL_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5761765.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10182..11345)
                     /gene="emrK"
                     /locus_tag="ACHKCL_000728"
     CDS             complement(10182..11345)
                     /gene="emrK"
                     /locus_tag="ACHKCL_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5761766.1"
                     /translation="MEQINSNKKHSNRRKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            11761..12375
                     /gene="evgA"
                     /locus_tag="ACHKCL_000729"
     CDS             11761..12375
                     /gene="evgA"
                     /locus_tag="ACHKCL_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5761767.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            12380..15973
                     /gene="evgS"
                     /locus_tag="ACHKCL_000730"
     CDS             12380..15973
                     /gene="evgS"
                     /locus_tag="ACHKCL_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5761768.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(16029..17170)
                     /gene="yfdE"
                     /locus_tag="ACHKCL_000731"
                     /pseudo
     CDS             complement(16029..17170)
                     /gene="yfdE"
                     /locus_tag="ACHKCL_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(17244..18188)
                     /gene="yfdV"
                     /locus_tag="ACHKCL_000732"
     CDS             complement(17244..18188)
                     /gene="yfdV"
                     /locus_tag="ACHKCL_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5761769.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(18258..19952)
                     /gene="oxc"
                     /locus_tag="ACHKCL_000733"
     CDS             complement(18258..19952)
                     /gene="oxc"
                     /locus_tag="ACHKCL_000733"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5761770.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(20006..21256)
                     /gene="frc"
                     /locus_tag="ACHKCL_000734"
     CDS             complement(20006..21256)
                     /gene="frc"
                     /locus_tag="ACHKCL_000734"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5761771.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(21769..22404)
                     /gene="yfdX"
                     /locus_tag="ACHKCL_000735"
     CDS             complement(21769..22404)
                     /gene="yfdX"
                     /locus_tag="ACHKCL_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5761772.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            22700..22975
                     /gene="ypdI"
                     /locus_tag="ACHKCL_000736"
     CDS             22700..22975
                     /gene="ypdI"
                     /locus_tag="ACHKCL_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO5761773.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(23052..23294)
                     /gene="yfdY"
                     /locus_tag="ACHKCL_000737"
     CDS             complement(23052..23294)
                     /gene="yfdY"
                     /locus_tag="ACHKCL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5761774.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            23647..24567
                     /gene="lpxP"
                     /locus_tag="ACHKCL_000738"
     CDS             23647..24567
                     /gene="lpxP"
                     /locus_tag="ACHKCL_000738"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5761775.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            24923..24994
                     /gene="ypdK"
                     /locus_tag="ACHKCL_000739"
     CDS             24923..24994
                     /gene="ypdK"
                     /locus_tag="ACHKCL_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5761776.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(25059..26297)
                     /gene="alaC"
                     /locus_tag="ACHKCL_000740"
     CDS             complement(25059..26297)
                     /gene="alaC"
                     /locus_tag="ACHKCL_000740"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5761777.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            26674..28371
                     /gene="ypdA"
                     /locus_tag="ACHKCL_000741"
     CDS             26674..28371
                     /gene="ypdA"
                     /locus_tag="ACHKCL_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5761778.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            28386..29120
                     /gene="ypdB"
                     /locus_tag="ACHKCL_000742"
     CDS             28386..29120
                     /gene="ypdB"
                     /locus_tag="ACHKCL_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5761779.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            29133..29990
                     /gene="ypdC"
                     /locus_tag="ACHKCL_000743"
     CDS             29133..29990
                     /gene="ypdC"
                     /locus_tag="ACHKCL_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5761780.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(29993..32488)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_000744"
     CDS             complement(29993..32488)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_000744"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5761781.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(32513..33550)
                     /gene="ypdE"
                     /locus_tag="ACHKCL_000745"
     CDS             complement(32513..33550)
                     /gene="ypdE"
                     /locus_tag="ACHKCL_000745"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5761782.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(33550..34635)
                     /gene="ypdF"
                     /locus_tag="ACHKCL_000746"
     CDS             complement(33550..34635)
                     /gene="ypdF"
                     /locus_tag="ACHKCL_000746"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5761783.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(34650..35897)
                     /gene="fryC"
                     /locus_tag="ACHKCL_000747"
     CDS             complement(34650..35897)
                     /gene="fryC"
                     /locus_tag="ACHKCL_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5761784.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(35919..36245)
                     /gene="fryB"
                     /locus_tag="ACHKCL_000748"
     CDS             complement(35919..36245)
                     /gene="fryB"
                     /locus_tag="ACHKCL_000748"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5761785.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(36464..37429)
                     /gene="glk"
                     /locus_tag="ACHKCL_000749"
     CDS             complement(36464..37429)
                     /gene="glk"
                     /locus_tag="ACHKCL_000749"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5761786.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            37633..38889
                     /gene="yfeO"
                     /locus_tag="ACHKCL_000750"
     CDS             37633..38889
                     /gene="yfeO"
                     /locus_tag="ACHKCL_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5761787.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            39004..39330
                     /gene="ypeC"
                     /locus_tag="ACHKCL_000751"
     CDS             39004..39330
                     /gene="ypeC"
                     /locus_tag="ACHKCL_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5761788.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(39470..40708)
                     /gene="mntH"
                     /locus_tag="ACHKCL_000752"
     CDS             complement(39470..40708)
                     /gene="mntH"
                     /locus_tag="ACHKCL_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5761789.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            41044..42246
                     /gene="nupC"
                     /locus_tag="ACHKCL_000753"
     CDS             41044..42246
                     /gene="nupC"
                     /locus_tag="ACHKCL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5761790.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(42296..44485)
                     /gene="pdeA"
                     /locus_tag="ACHKCL_000754"
     CDS             complement(42296..44485)
                     /gene="pdeA"
                     /locus_tag="ACHKCL_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5761791.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(44693..44768)
                     /locus_tag="ACHKCL_000755"
     tRNA            complement(44693..44768)
                     /locus_tag="ACHKCL_000755"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44733..44735),aa:Ala,seq:ggc)
     gene            complement(44808..44883)
                     /locus_tag="ACHKCL_000756"
     tRNA            complement(44808..44883)
                     /locus_tag="ACHKCL_000756"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44848..44850),aa:Ala,seq:ggc)
     gene            45104..45463
                     /gene="yfeC"
                     /locus_tag="ACHKCL_000757"
     CDS             45104..45463
                     /gene="yfeC"
                     /locus_tag="ACHKCL_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5761792.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45465..45857
                     /gene="yfeD"
                     /locus_tag="ACHKCL_000758"
     CDS             45465..45857
                     /gene="yfeD"
                     /locus_tag="ACHKCL_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5761793.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(45897..>46139)
                     /locus_tag="ACHKCL_000759"
     CDS             complement(45897..>46139)
                     /locus_tag="ACHKCL_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5761794.1"
                     /translation="LAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT        12406 a        11459 c        10679 g        11595 t
ORIGIN      
        1 aggtttaaaa attgtgatac ttatgacaga tttgagtgac attttatcaa tctactgatt
       61 ttgatggtga aatttattag aaggtatgcg tagtgctacg ggagaaatga gaaacgcgcc
      121 cagcaatggc gcatcctttc ttaagcaagt tggctagtat ctatagcatc catccaatca
      181 ggagcaattc ccaaacgttc cgcgaactct tcattggtca cgtcttccat tgagagacta
      241 gccagacaat cgttaatttc agaatagacg ggggaaagta ataggtattc ccgcaactct
      301 ttcaggtctt tttccatctt aacactcctt gttaatagac cgtcgaacaa tggtacagat
      361 ttgtaccatt ccaagtactg gcaagcaacc ccaccagcac aacaaattat ctcatacggt
      421 aaaaacggtg tcgggctgtt tctttcggct agctcaatat atcaccttaa acaccagccg
      481 ttatcattgt ctcaattggg tataccgtaa tgacaaggtg atacaaagtc tcaatagggt
      541 tgtataggaa cattttgact acacaaactg cattatgtat catttgcact tatattcaca
      601 acccttttga gacaatgcaa tgaacgttag aatttactgc cgagcatcaa ctgaggggca
      661 acacgctgat cgagcactta ccagcctacg tgaattttca aaatcaaaag gctggcagat
      721 tgcaggagag tatatagaaa atgctagcgg ggcgaagctt gagcgcgtag agcttatgcg
      781 gctattgtct gaagcccagt ccggcgatct gttgctagtg gaagctatag atcgtctgag
      841 tcgcctagaa catagtgcat gggtcgaact gaaagatacg ctcaaccgaa aagggttaat
      901 catcgtatca atggatttac caacgagctg gcagatggtc gagatggcag gcaatgatct
      961 aaccagcggc atcctgagag ccgtaaatgc catgttgatt gatatccttg ccacaatggc
     1021 acggcaggat tacgaaacac gccgtaaacg ccaacagcaa ggcattgaac gagctaaaag
     1081 cgaaggcata tacattggga gggcgaaaaa tcaggaagcc cgagagatcg ttagagagat
     1141 gttggagcaa ggagtaaaac ctgaactcat catgaaggca gcaggaatca gccgagcgac
     1201 ttactaccgg ataaaaaacg agctattgat cgtaaaatca gaataaaatt tgaaaggtcg
     1261 tcaggcttgt ttcatcaggt aaaaaccagc atttctgatc acaacgcttg ggggcaccta
     1321 ttggggcact caccaaaatc gaaagagaaa taaactacaa ttcaaattgt tagctaattt
     1381 ttcgattcct gcagggacac cagataccct tcaaaccaaa tctaccttca tcccgtaaac
     1441 gatgggtttg gcagcacact tgccctatat ctactcattt ttactgcaac aggttgaaat
     1501 ctcagcactg tcagaaagcg ctgatgacta aacagcccag ggccgggcga tgtaaccatc
     1561 acacagaatc ctgatagcga aatacggcgt gactcgatac ttcactccgc aatgcattcc
     1621 ttgatgaatt cgcaggcccg tgatacacgg gacaggtcgc agaatgacga caatgtcctg
     1681 gaaatcagcg aaccgtgtat ccggagtaca tttgagcgac tgtaccagaa catgaatgag
     1741 gtgtttggat taggcgatta ttagcagggc taagcatttt agtattatta ttttccggtt
     1801 gaaggatatg gagatatcga caacaaccgg aaaaagttta cgtctatatt gctgaaggta
     1861 caggcgtttc cataactatt tgctcgcgtt ttttactcag gaagaaaatg ccaaatagca
     1921 acatcaggca gacaataccc gaaattgcga agaaaactgt ctggtagcct gcgtggtcaa
     1981 agagtatccc agtcggcgtt gaaagcagca caatcccaag cgaactggca atttgaaaac
     2041 caatcagaaa gatcgtcgac gacaggcgct tatcaaagtt tgccacgctg tatttgaaga
     2101 cggatatgac acaaagtgga acctcaatgg catgtaacag cttcactaat gaaataatcc
     2161 aggggttaac gaacagcgcg caggaaagga tacgcaacgc cataatcaca acaccgataa
     2221 gtaatgcatt ttttggccct acccgattca caaagaacgg aataatcgcc atgcacagcg
     2281 cttcgagtac cacctggaat gagttgagat aaccatacag gcgcgttcct acatcgtgtg
     2341 attcgaataa acctgcataa aagacaggaa aaagttgttg atcaaaaatg ttatagaaag
     2401 accacgtccc aacaataaat atgacgaaaa cccagaagtt tcgatccttg aaaactgcga
     2461 taaaatcctc tttttttacc cctcccgcat ccgccgctac gcactggtga tccttatctt
     2521 taaaacacat gttgatcatc ataaatacag cgccaaatag cgagaccaac cagaagttga
     2581 tatggggact gatactaaaa aatatgccgg caaagaacgc gccaatagca tagccaaaag
     2641 atccccaggc gcgcgctgtt ccatattcga aatgaaaatt tcgcgccatt ttttcggtga
     2701 agctgtcaag caaaccgcat cccgccagat accccaggcc aaaaaagagc gcccccagaa
     2761 ttagacctac agaaaaattg ctttgcagta acggttcata aacgtaaatc ataaacggtc
     2821 cggtcaagac caggatgaaa ctcatacacc agatgagcgg tttcttcaga ccgagtttat
     2881 cctgaacgat gccgtagaac atcataaata gaatgctggt aaactggttg accgaataaa
     2941 gtgtacctaa ttccgtccct gtcaacccta gatgtccttt cagccaaata gcgtataacg
     3001 accaccacag cgaccaggaa ataaaaaaga gaaatgagta actggatgca aaacgatagt
     3061 acgcatttct gaatggaata ttcagtgcca taattacctg cctgtcgtta aaaaattcac
     3121 gtcctattta gagataagag cgacttcgcc gtttacttct cactattcca gttcttgtcg
     3181 acatggcagc gctgtcattg cccctttcgc cgttactgca agcgctccgc aacgttgagc
     3241 gagatcgata attcgtcgca tttctctctc atctgtagat aatcccgtag aggacagacc
     3301 tgtgagtaac ccggcaacga acgcatctcc cgcccccgtg ctatcgacac aatccacaga
     3361 cattccagca aaatggtgaa cttgtcctcg ataacagacc accacccctt ctgcaccttt
     3421 agtcaccaac agcatggcga tctcatactc ttttgccagg gcgcatatat cccgatcgtt
     3481 ctgtgttttt ccactgataa gtcgccattc ttcttccgag agcttgacga catccgccag
     3541 ttgtagcgcc tgccgcaaac acaagcggag caaatgctcg tcttgccata gatcttcacg
     3601 aatattggga tcgaagctga caaaacctcc ggcatgccgg atctccgtca tcgcagtaaa
     3661 tgcgctggta cgcgaaggct cggcagacaa cgcaattgaa cagagatgta accattcgcc
     3721 atgtcgccag cagggcaagt ctgtcgtctc taaaaaaaga tcggcactgg ggcggaccat
     3781 aaacgtaaat gaacgttccc cttgatcgtt cagatcgaca agcaccgtgg atgtccggtg
     3841 ccattcatct tgcttcagat acgtgatatc gactccctca gttagcagcg ttctttgcat
     3901 taatgcacca aaaggatcat cccccacccg acctataaac ccacttgttc cgcctaatct
     3961 ggcgattccc accgcaacgt tagctggcgc gccgccagga caaggcagta gccgcccgtc
     4021 tgattctggc aagagatcta cgaccgcatc ccctaaaacc catactttgg ctgacatttt
     4081 tttcccttaa attcatctga cttacgcata gtgataaacc tctttttcgc aaaatcgtca
     4141 tggatttact aaaacatgca tattcgatca caaaatgtca tagttaacgt taacatttgt
     4201 gatattcatc gcatttatga aagtaaggga ctttattttt ataaaagtta acgttaacaa
     4261 ttcaccaaat ttgcttaacc aggatgataa aaatgacgca atctcgattg catgcggcgc
     4321 aaaacgcact agcaaaactt cacgagcgcc gaggtaacac tttctatccc cattttcacc
     4381 tcgcgcctcc tgccgggtgg atgaacgatc caaacggcct gatctggttt aacgatcgtt
     4441 atcacgcgtt ttatcaacat cacccaatga gtgaacactg ggggccaatg cactggggac
     4501 atgccaccag cgacgatatg atccactggc agcatgagcc tattgcgcta gcgccaggag
     4561 acgagaatga caaagatggg tgtttttcag gtagtgctgt cgatgacaat ggtgtcctct
     4621 cacttatcta caccggacac gtctggctcg atggtgcagg taatgacgat gcaattcgcg
     4681 aagtacaatg tctggctacc agtcgggatg gtattcattt cgagaaacag ggtgtgatcc
     4741 tcactccacc agaaggaatc atgcacttcc gcgatcctaa agtgtggcgt gaagccgaca
     4801 catggtggat ggtagtcggg gcgaaagacc caggcaacac tgggcagatc ctgctttatc
     4861 gcggcagttc attacgtgaa tggactttcg atcgcgtact ggcccacgct gatgcgggtg
     4921 aaagctatat gtgggaatgt ccggactttt tcagccttgg cgatcagcat tatctgatgt
     4981 tttccccgca gggaatgaat gccgagggat acagttaccg aaatcgcttt caaagtggcg
     5041 taatacccgg aatgtggtcg ccaggacgac tttttgcaca atccgggcat tttactgaac
     5101 ttgataacgg gcatgacttt tatgcaccac aaagctttgt agcgaaggat ggtcggcgta
     5161 ttgttatcgg ctggatggat atgtgggaat cgccaatgcc ctcaaaacgt gaaggctggg
     5221 caggctgcat gacgctggcg cgcgagctat cagagagcaa tggcaaactt ctacaacgcc
     5281 cggtacacga agctgagtcg ttacgccagc agcatcaatc tatctctccc cgcacaatca
     5341 gcaataaata tgttttgcag gaaaacgcgc aagcagttga gattcagttg cagtgggcgc
     5401 tgaagaacag tgatgccgaa cattacggat tacagctcgg cactggaatg cggctgtata
     5461 ttgataacca atctgagcga cttgttttgt ggcggtatta cccacacgag aatttagacg
     5521 gctaccgtag tattcccctc ccgcagcgtg acacgctcgc cctaaggata tttatcgata
     5581 catcatccgt ggaagtattt attaacgacg gggaagcggt gatgagtagt cgaatctatc
     5641 cgcagccaga agaacgggaa ctgtcgcttt atgcctccca cggagtggct gtggtgcaac
     5701 atggagcact ctggcaactg ggttaacata atatcaggtg gaacaacgga tcaacagcgg
     5761 gcaagggatc cgcgtcacac ttcccccttc acgaccttca ataatatgca atgcagcttc
     5821 ccgcccgata atgtcatgtg gaagctgaat tgtggtcagc ggcggtaaaa acagatgccc
     5881 gacgccaacc agattatcaa agcccattac ggcgacatcc tgcgggatac gtaccccctt
     5941 agccaggaga acctgataag ccacaaaggc tgcgcgatcg ttaccacata tcagaacatc
     6001 aaaatctggt ttgcccgatt tgaagtggtc attgagtaaa cttgcgagat cggtgtagtg
     6061 atcatcacct gttgccatgt gaaattgttt cacctcagcc agatctcgtc cagcatcacg
     6121 ccaggcctgc tcaaatccct gccgacgata ccctgttgcc aacgcacttt ccggtagcca
     6181 gaagcataac ggttgacgat agcccgccgc gagcaaatgc tgtgttgatt catattgtgc
     6241 agtgtaatca tcagggatat aactgggtaa cgctgggtca tccgccacac agttcgccaa
     6301 tacaatattt tcaccataca gagactcagg tagcgtgata tgtcgcagcc ccattgtagt
     6361 atagataatg ccatccggac ggtgggcaag cagctgacgt gccgcgcggg cagcgtcatc
     6421 ttcagaaaaa atattgatta aaaaactatt ccagccgaac tcgctggcgg tttgctcaat
     6481 ggcaagcaga atatcaacag agaaaggagt ggtagcagtg tcctgcgcca gcacggcgag
     6541 agtcgacggc ttacgtcctt gagcgcgcat cttacgggcg gaaagatcag gaacataatt
     6601 cagggtctgg attgcctgca atacgcggtc acgcgttgca ggacgcacag attctgcatt
     6661 atgcatcacc cgggagactg tcatcatcga cactcccgcc aggcgtgcga catcctttaa
     6721 tgaagccata cccaagccgt ttgccgtaaa acgggcactg tagcagaaac agacgtcact
     6781 ggcgagatcc aacgaaattc tcgacgccct atcacccgac acggcaatac aataaaaaat
     6841 aacaataatt gccggataat tgtacccaat tccgcctctg ttctcgccaa cgagtgacca
     6901 tataggcaag taagaaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa
     6961 tatggatcat tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta
     7021 tcggttcagg cgcgattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc
     7081 aatattgcgg cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg
     7141 cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag
     7201 acgtactatg gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga
     7261 tctggttgct cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg
     7321 tttaccttat gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg
     7381 ttttgcaccc tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga
     7441 atcagaactg gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc
     7501 gatcagcggg caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa
     7561 agcacgcggc gggatttatg aagtcctggc acatgcagaa aaactggctc tggaagcggg
     7621 gttgctgacg cttgaagatg actacagcaa actgctttct ccggagttta aacagttctt
     7681 tagccagtac agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat
     7741 gagcgcccgc attggcttta aggtgacagt gcatatgtct gctgatgccc gggcatggaa
     7801 aaaagcgaaa ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt
     7861 tgccgtcgag gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga
     7921 cgagaattcc cgcacgttgt tccttgggta ttccgtcgca ggccagcgtc ttaaagcgca
     7981 atttgccgag caaggtcgta ttgtcgatgc tgataaccct ctgtttgtct atctgccgtg
     8041 tggtgttggc ggtggtcctg gtggcgtcgc attcggactt aagctggcgt ttggcgatca
     8101 tgttcactgc ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac
     8161 aggattacac gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga
     8221 tggccttgca gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga
     8281 tggcttctat acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga
     8341 agaaggtatt cgtcttgaac cttcggcact ggcgggtatg gccggacctc agcgcgtgtg
     8401 tgcatcagta agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaatgccac
     8461 tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa gaagagatgg agcaatatct
     8521 ggcaaaaggc cgttaataac gtttcaacgc agcatcgcaa tcctttccct gggtgagcga
     8581 tgctgccgat ggcgcagact taagatcccc agtcttaccc gctataaccc cctgctttta
     8641 ccatcaccca acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag
     8701 taagataaaa gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg
     8761 ctgggttatt tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc
     8821 cataatttgt gacgatgaat taaacacggg gttaaattga tcgatggttt ctgtcaactg
     8881 actatggtgt aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga
     8941 tcctgacaag gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg
     9001 caagcctgaa aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg
     9061 aaaaaactgc ggcaaaatga tacctgtaaa atcaatcgtt ggcataaatg tcacagaacg
     9121 ccagtaatag caaaccgcat acatcaaaaa actgaatgtc accaacaccc gcatgtctat
     9181 tttgttgcca taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc
     9241 ataagcaagt ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat
     9301 aaggacgatc gctccagagt aaaataaata cgcgcatgtg atactcacaa taccaatggt
     9361 gaagttacgg gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca
     9421 aatgactaaa gagatcagaa aaataactga tactactgtt aatattatta ttgtactcga
     9481 gttgaaccaa tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag
     9541 cactaacagg gtcagtcctg gtagattcat tttgaccggt gaagtctcag tttctcttcc
     9601 tttaagtaag gttaagcata atgtcaggac gataatcccc atagggacat tgattaaaaa
     9661 tatccaaccc cagctaaagt tatcacaaat ataaccgccc aatatcggcc cacatatcgg
     9721 agcgataatc acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata
     9781 attccttaat aataaactct gtgacagtgg aattaacggc cctgccatta acccctgaac
     9841 gactctaaaa aatatcagca catcaagatt gatcgataaa ctacacatta aggaagacag
     9901 cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct
     9961 gccggtaaca gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc
    10021 ttcgtctgtt gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt
    10081 ggaatccaac atttgcataa acgtcgctaa tgacaatgca atagtgacgc accataacgt
    10141 cccaccggtt aatggtgccg gagttgattt agtgattgcc attaaagttg tccattatgc
    10201 gaaataatgt tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg
    10261 gtataagccg gcatggacgt cacggttgaa gccagctcag gcatctcagc aatgtcttcg
    10321 ttcttcgtat caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt
    10381 tcttttggat caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct
    10441 gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc
    10501 acccggccat gaaacacaac attctcacca taaagatcgc tgataatatt gaccgattga
    10561 ccaatccgta catccgtgag ttgtgtttct ttaaagttag cattaaccca catttgacgt
    10621 gccggtacga cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactt
    10681 ctctgggcaa tatagccggt aaccggactc ttaatatccg tacgtttaag cgccaaccag
    10741 gcttctttgg ttgcatccgc agcttcaacg acttgtggct gacggtttaa tggtgtgttc
    10801 attactaaag ctttattcgc tttataagcc tggatagcgg cattcaatgc cgctttgcta
    10861 cttattaacg tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct
    10921 aaaggcacac gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg
    10981 acttcggcac tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt
    11041 gccagattat ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact
    11101 aaaatgtcac cctgtcgaac gtagttcgta tctttatgat taacgacagt gacactacct
    11161 gagacttgtg cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc
    11221 atgtcttcta attccattga ccaataggca taagcacctg aaaacgcaat aaataaaact
    11281 atcaccaata aagaaaagta ttttcttctg ttagaatgtt ttttatttga attaatctgt
    11341 tccactatta tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtattc
    11401 attcttcata tcaaacacag tgcatgtgtt gcacattatc cctggaacgt tggaggccta
    11461 atttaaatga ggtgcaaaaa aaacaacaga ggactaaacc gtggcttttg caatacaatt
    11521 cttacgcctg taggattagt gagaagactt atagtgctaa cttgaaacta taaatcatcg
    11581 gtataatccc tgattttatt gttgacattt catatatgcc gactatttat atggtatact
    11641 tgtcgaatta tcttaaagga agctcaaatt ttcttatttt tattgagaaa atgagatgat
    11701 gccttatgtc tgtattacta cagggagaag ggagatgctt cattgcaaag ggaataatct
    11761 atgaacgcaa taattattga tgaccatcct cttgctatcg cagcaattcg taatttattg
    11821 atcaaaaacg atattgaaat cttagcagag ttgactgaag gcggaagtgc cgttcagcgg
    11881 gtggaaacac ttaagcctga tatcgtcatc attgatgtcg atatccccgg agttaacggt
    11941 atccaggtgt tagaaacgct gaggaagcgc caatatagcg gaattattat tatcgtctcc
    12001 gctaaaaatg accattttta cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg
    12061 agtaaaaaag aaggcatgaa caatatcatt gcggctattg aagctgcaaa aaatggctac
    12121 tgctatttcc ccttctctct caaccggttt gttggaagtt taacgtccga ccagcaaaaa
    12181 ctcgactcct tatcgaaaca agaaattagt gtcatgcggt atattcttga tggcaaggat
    12241 aataatgaca ttgctgaaaa aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt
    12301 cgcctgatgg aaaaattaga atgtaaatca ctgatggatc tttacacatt cgcacaacgt
    12361 aacaaaatcg gctaaccaca tgaagttttt accctatatt tttcttctct gttgtggtct
    12421 ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa
    12481 caaccgtgtc acacttgatc cactacgtct gagcaacaag gaattacgtt ggttagcgag
    12541 caaaaaaaat cttgtgattg cagtacacaa gtcccaaacg gctacgttgt tgcataccga
    12601 ttcgcagcaa cgggttcgtg gtattaatgc tgattattta aatcttttaa aaagagcgtt
    12661 aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg acgcgctgga
    12721 agaaggtgaa gtcgatatag tgttatcaca tttagttgct tcgccgcctc ttaatgatga
    12781 cattgctgca accaaaccac tgataattac ctttccggcg ctggtcacca cccttcacga
    12841 ttcaatgcga ccgcttacct catcaaaacc agtaaatatt gctcgagtag caaattatcc
    12901 ccctgacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt ttacaaattt
    12961 atatcaggca ttagcatccg tctcagccgg acagaatgat tactttattg gtagtaacat
    13021 cattaccagc agtatgattt cccgctattt cactcactcc ttaaatgtag tgaaatatta
    13081 taactcaccg cgtcaatata acttcttctt aaccagaaaa gaatctgtca ttcttaatga
    13141 agtactcaat cgatttgttg atgctttaac aaatgaagtt cgctatgaag tatcacaaaa
    13201 ttggcttgat acaggaaacc tggcctttct gaacaagcca ttagaactca ctgaacatga
    13261 aaaacagtgg attaagcagc atcccgattt aaaggtgctg gaaaatcctt actcgccccc
    13321 ctattctatg acggatgaaa atggctcggt tcggggcgtt atgggggaca ttcttaatat
    13381 tattaccttg caaacaggtt taaatttttc tccgatcacc gtttcacaca atatccatgc
    13441 tggaacacag ctgaatcccg gcggatggga tatattaccc ggtgctattt atagtgaaga
    13501 tagagaaaat aatgttttat ttgctgaagc cttcataaca acgccttacg tttttgtcat
    13561 gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg ccattccata
    13621 ttattatgag cttcattcgc aattaaaaga gatgtatccg gaggtagagt ggataaaagt
    13681 cgataacgcc agcgctgcat ttcacaaggt caaggaaggc gaacttgatg ctctggtcgc
    13741 gacacagtta aattcacgtt acatgatcga ccattactat cctaatgaac tgtatcattt
    13801 tcttattccc ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga
    13861 acttaaggat attattaata aagcactgaa tgcaatcccc ccaagcgaag ttctgcgcct
    13921 gaccgaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc tctatagcga
    13981 gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc ttttatgggg
    14041 attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca
    14101 aatatcattc cggaaagcgc tctcggactc cttaccgaat ccaacttatg ttgtaaactg
    14161 gcaaggtaat gtcattagtc acaatagtgc atttgaacat tatttcactg ctgattacta
    14221 caaaaatgca atgttgccat tagaaaacag tgaatcaccc tttaaagatg ttttttctaa
    14281 tacgcatgaa gtcacagcag agacgaaaga aaaccgaaca atatacacac aggtatttga
    14341 aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat gtaatctgcc
    14401 agccagcgaa catgctgttt atatttgtgg ttggcaagat attaccgaga cgcgtgattt
    14461 aattcatgca ctcgaagtag agagaaataa agcgatcaat gcaactgtcg caaaaagtca
    14521 gtttctggca acaatgagtc acgaaataag aacaccaata agctccatta tgggcttcct
    14581 ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga tttcactagc
    14641 ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg tcgacaaaat
    14701 tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt tagtccagaa
    14761 cacttgtcac tctttcggtg cgattgctgc aagcaaatcg atcgcattaa gttgcagcag
    14821 tacatttcct gatcattacc tggttaagat cgaccctcag gcgtttaagc aggtcttatc
    14881 aaatttactg agtaatgctc tcaaatttac caccgagggg gcagtaaaaa ttacgacctc
    14941 cctggttcac attgatgaca accacgctgt aatcaaaatg acgattatgg attctggaag
    15001 tggattatca caggaagaac aacaacaact gtttaaacgt tatagccaaa caagtgcagg
    15061 tcgtcagcaa acaggttctg gtttaggctt aatgatctgc aaagaattaa ttaaaaacat
    15121 gcagggtgat ttgtcattag aaagtcatcc aggcatagga acaacattta cgatcacaat
    15181 cccggtagaa attatccaac aagtggcggc tgtcgaggca aaagcagaac aacccatcac
    15241 actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca ggctattact
    15301 caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg gtgtgcaagc
    15361 gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta atatgccgaa
    15421 tgtggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcgtcct tacccatctg
    15481 ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaaatt gcggcatgaa
    15541 cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa gtcagttaca
    15601 ccaggttgcg catattgtac ctcagtatcg ccaccttgat atcgaggccc tgaaaaataa
    15661 tacggcgaac gatctacaac tgatgcagga gattctcatg actttccagc atgaaacaca
    15721 taaagattta cccgctgcgt ttcatgcact agaagctggc gataatagaa ctttccatca
    15781 gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt tgattaatat
    15841 tagccatcag ttagaaataa cacctgtttc agatgacagt aagcctgaaa ttcttcagtt
    15901 gctaaactct gtaaaagaac acattgcaga gctagaccag gaaattgctg ttttctgtca
    15961 acaaaataac taaatagtgg ctcccacaat gttcaaatgt gggagctatt taccagcaca
    16021 tcttacattt atgatgagaa ctcctggcga atttgctccc catgctggtc gagcgttgcc
    16081 gctcccggca taacatgcgg gtccgcgcag ccactgattt ttatcggatt acccggcatc
    16141 attattcccc cggcttcaat caacatattt ctcgcctgag tttgtggcaa attaatggcc
    16201 tcagccacac ttaataacgg cgcgacgggc acaccaactt catgtattct ggctaaccaa
    16261 acttcagctg cctgtgtttt taacgtccgc tcaatatatt gtttaagaat agtctggttt
    16321 tgtacgcgta aaatattgct gctaaatctg ggatcattaa ccagttccgt aagctccagt
    16381 gcctggcata acgcagagaa aagcttgtca ttaccacaac aaatcgtaat cggcttatcc
    16441 tgagtatcga aaacatcaaa aggtgccatg taggggtggc gatttcccag gcgttgtggt
    16501 gacttccctg tcgcaatata tgccatcaga ccatgctcca gaaaactcag cgtggcatca
    16561 aacatcgcta tatcgacatg cgcccctctt tggctctttt cgcggccata aagtgcactc
    16621 actattccgc tgaataaata aacaccgccg catagatccg caagagaggt accaacacgc
    16681 actggcggag catcagggta ccctgtttcc atcattatcc cgctcattgc ctgaatgatg
    16741 gtatcgtagg caggagcatc ttttagcgga ccggtatgtc cgaaacctga cgatgaagca
    16801 tatatgaggc gcgggttgat ttcttgtaac ctttcccatg aaagccccag tttttccatt
    16861 gtacctgggc gaaaattctc agctaataca tcagcttgtt taagcatatt tataaatata
    16921 cttttatcgt gatcattctt taaatcaaga accacactct ctttaccatg attaataaaa
    16981 ctgtaataga gtgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacccg
    17041 gcggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc
    17101 cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat
    17161 tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta
    17221 tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga
    17281 gccgtgacga caaaacccag tacgctcaca gccaatgacg ctgtaccagt gcgggtgtag
    17341 acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt
    17401 accatcatct gcagatgttc gctgttcaaa tgacatgcca taccaacaag aagcagcgcc
    17461 agtggcatca gaatcagctt caggaaggtg ttataagcaa tttcggcact gaactcgaat
    17521 ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca
    17581 atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccaa caccaggatc
    17641 gttgccagaa caggtgccca tacaactggc tcctttgccg cagaaattaa tgcgctcaga
    17701 ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga
    17761 atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa
    17821 tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacctgca
    17881 attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac
    17941 caggagaaaa agaaacatcc gacaataacc acgagtgata caagggtcag acgagtgtcc
    18001 gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagggctgc aggaagcgcg
    18061 tagtttaata ccagtttatt aaatgcccgg gcttgatctt ctgaaaaagt ttctcgtctg
    18121 ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat
    18181 gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga
    18241 ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg
    18301 ccactttcag ttcctgctgc agggtcgatg accacattaa taatggtcgg tttgcgcgac
    18361 tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg
    18421 ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc tgttggtgat
    18481 ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa
    18541 ataacgatcg ttaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa
    18601 ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaggtcac gctagcaccg
    18661 atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt
    18721 ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact
    18781 aaataaatat cctggttctc acgcaataca tcgcgcacag cacttaatgc attaaagtaa
    18841 tttaatggtt gggtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg
    18901 tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagctct
    18961 gccagcatac cttgcatact ggatgcaata tcgccaacga ctggcacagc aatggggcgg
    19021 ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat
    19081 ccttttttac cgtgtgccag taaccaattc agtcgtgcac caacaagcat gacaacgtca
    19141 gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcgagg
    19201 atccctttcg ccatagacat tggcaggaat ggaatctggg cactttcaat aaattcacga
    19261 agctgttcgt cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca
    19321 gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc
    19381 gacggatttt caacttttac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc
    19441 aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac tgagacccgg
    19501 atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg
    19561 gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg
    19621 acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg
    19681 ttggccaatg cggtcaaacc attgaggaat cctggcgcag acactgtcag gcagatccct
    19741 ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga
    19801 aaaccaatat aacgaatgcc ttccgcctgg gcatggcgcg ccatatccgt cacaggaata
    19861 cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc
    19921 ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat
    19981 tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt
    20041 catcgtcgct ataacccagc tcctgcaata ccgcagcggt atgttcacct aatagaggcg
    20101 cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaact gtcagatatt
    20161 ttccacgcaa tggttgttcc acttcgacaa cactgccgct ttggcgtaaa gagggatcaa
    20221 gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaataag
    20281 ccactgcttc atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga
    20341 aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc caatctggtt
    20401 tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg
    20461 cgttaggatc agtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac
    20521 ccgcattacc tccgcgggga actgcatcac caaatgtgcc attcggatac tgcgggtatt
    20581 cttccagata acccaattta tcgagacgct gctggtcgcg taatttcacg cggcaaaggt
    20641 tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat
    20701 gcagcaaagc agcaagtaaa ccgatcagta aatgcattcc ggtgttgctg tcacccaacg
    20761 ctgcagcgct taccagcggc ggaccgtccc aaaaacccgt agtggatgcc gcgccacccg
    20821 ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc
    20881 ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc
    20941 ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca
    21001 gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt
    21061 tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc agctggtgac
    21121 gcgttacgtc accaacgccg gggcgttcaa ttttaatgac gtcagcgcca aaccaggcca
    21181 gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt
    21241 gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa tttagcgttt
    21301 atttaaggca taacgccctc tataaataat ccgcaaaata tcgtcatagc ggcactcgat
    21361 atttttaaat tcctgtatca agtgctgaaa atcatttcat cgctacttcc aattcctttc
    21421 tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattggttc gtggtaatca
    21481 gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatttac
    21541 aagtaagggt atgattacgc tttttagagc gtaccataaa atataacata ttaaatgatt
    21601 tgtgatgaca cgggattatt gcggtttact ttgaggaata taaaatgcga gtttatagaa
    21661 cagcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt
    21721 cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt agttcacaaa
    21781 cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc
    21841 gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt
    21901 aagcggcatc agatattggt tttccattac accaacaccg gccaggcgaa gctcttccat
    21961 tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg cggcttcttt
    22021 ttcgggtgtc gctacataac tctcagaaat tccgacagat gcattaatga caatatattg
    22081 gtcatcattg acattggtct ttttacctgg cttagcgaac tttgcccatt cggtactgtc
    22141 atctgacaac aaagctgaag cttcattggt cagttctttt gccttttccg gatcgccatg
    22201 aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat
    22261 acgctctgct gcagctgccg ttttctgggt ttgctgaact tgctgagttt gctgttgtgc
    22321 cgctaccgga gcattatcag cagcccatac agttgaagat gccagaatag ctgtgaccat
    22381 cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg
    22441 ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt
    22501 tatcttacag ttgtagttat tatcgcaaga agtattttag tcttacaatt gtaggaatat
    22561 gcttcctgtc acagagaact attcttatgt accacaaaga gatgtcattt attatatcga
    22621 tgtaaatatt gattaattat atgtaaatac agccataaca tcgtacattc tcagtgaacc
    22681 attttcagga taactttcta tgaaagtaaa cttaatactt tttagcttat ttttattggt
    22741 ctctattatg gcatgcaatg tttttgcatt ttctaattct ggtggtggta gtgagacgag
    22801 ctataaagag ccagaaaaaa cgtcagcgat gacgacaact cactctacaa aatttcagcc
    22861 atcacaggcg attttgttta agatgagaga agatgcacca ccattaaacc tcacagaaga
    22921 aaccacgccc ccttttccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct
    22981 gtcacctggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgaacc
    23041 atcctgaccc actatctcct gccatacccc accataaagc gaatcatcaa gccacaagcc
    23101 ggggcaagaa acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg
    23161 aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc
    23221 acgttcagta cctgaccgat gtatccgctt acgcagacaa tacacagggc gagaaacatc
    23281 cataaataaa acatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt
    23341 taatagtaag acaatcctta acctctggca atttttgcta agcgtcaatg aaaacagcat
    23401 gttaaatgca atactattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta
    23461 gctgaaagca gtcagccagt gataaagtac agataccgca ttcatgctcc tcgtggttat
    23521 gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa
    23581 ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag
    23641 gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc
    23701 acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc
    23761 tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacggcg tgaatctatc
    23821 gcccgtaaaa acctggaact ttgcttcccg cagcattctg cggaagaacg cgagaagatg
    23881 attgccgaaa actttcgttc actcggcatg gctctggtag aaaccggcat ggcgtggttc
    23941 tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggctggataa ccttaaacgc
    24001 gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg
    24061 ggcggccgcg tgatgggact ttgtcagcca atgatggcta cctatcgtcc acataataat
    24121 cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc
    24181 agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct
    24241 cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat
    24301 gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc
    24361 gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg
    24421 gaaggctacc cgacagatga aaatcaagcc gctgcctata tgaacaagat tatcgagaaa
    24481 gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg
    24541 gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg
    24601 gccataataa cctcatggtt tttatggctt ttttcttaag attttttcct tcgctgaata
    24661 tctaattatt accgccagaa attaaactgt cgccgatatg agacacctgt aagttactaa
    24721 aagtaaataa gaaaatgcct cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct
    24781 cgagcagttt agctcagaat tctggggtga tgcccacggt aaaatacagg acttttaagg
    24841 tcgttttgcc cggtgtcaac tcactgtatc aggtgtgatg aagtcattca ggcgtaaccg
    24901 taattacgcg gagagatgta aagtgaaata tttctttatg ggcatttctt ttatggtcat
    24961 cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg
    25021 agccatcagg ctcctgtttg catttctgac gacggtgttt attccgcgtt ttcgtgaata
    25081 tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca tcgctttaat cccacgaatc
    25141 gcctgacgaa tacggtcgcg gttttcaatc agtgcaaagc gaacatgcgt gtcgccgtag
    25201 tcaccaaagc caatccctgg agagacacag accttcgctt cgttaaggag cttcttggca
    25261 aattccagcg atcccatggc cgcatatggt tccgggattt tcgcccagac atacatcgaa
    25321 gccttcggca tttcgaccat ccagcccgct tcatgcagcc ctttaaccaa tacatcgcgg
    25381 cggcgtttgt actgttcggc aatgtcgcgc acgcactgtt gatcgccctc cagcgccgca
    25441 atcgccgcca cctgcaacgg cgtaaaagtg ccgtaatcgt gatagctttt gattcgcgcc
    25501 agggcactga ccagcgtttt gttgccgacc ataaagccga tacgccagcc cgccatgttg
    25561 tagcttttcg acagcgtaaa gaactcgact gccacatcgc gtgcacccgg tacctgcatg
    25621 attgacggcg ctttccagcc atcgtagacg atatcggcat aggccaggtc atggaccacc
    25681 agcacatcgt agcgtttcgc cagcgccact actttttcga agaactccag ttccacgcat
    25741 tgcgcggttg ggtttgacgg gaagccgagg atcatcatct tcggtttcgg ataactttca
    25801 agaatggcgc gctccagttc gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt
    25861 acctgcgccc cggcaatcac cgcgccataa atatggatcg ggtagcttgg attcggcacc
    25921 agcaccgtgt caccatgatc cagcgtcgcc agcatcaggt gtgccagccc ttctttcgaa
    25981 ccaatggtga cgatggcttc tgattccgga tcgatttcaa cgtcgtagcg atcctgatac
    26041 cagcgggaaa tggcgcgacg taaccgcgga atgccgcgtg aagtggagta accatgcgtg
    26101 tccggacgct gggccacagt acataatttt tcgacgatat gcggcggagt cgcaccgtcc
    26161 gggttaccca tgctgaaatc gataatatct tcgccgcgcc gacgcgcagc cattttcagt
    26221 tcagcggtga tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca
    26281 gggcgagtgt cagccataat ttcctcagat taacgttagc gcccggaccg tccgagcgac
    26341 gctgccacga tggtggctgt tttgaaaata gcctgattaa tttctgtctg tccagcgttt
    26401 tagaacattt ttattaatga cgttaaatga ggaactctgc tttaaaaaca ggaaatttat
    26461 ctgcgctaac aagaaaatta ccactcattt tattaacata ataatttcaa taaatttact
    26521 aaccagaagt gcattatcaa gagatgcatt gctaatacac catttatcta tcaatcccct
    26581 tttaaaattg tggtttttcc tcaaaaacgg cctgctggtc atcagccttc aggttaccta
    26641 tcatagaggt ttaatcctta ttcagagtca cccgtgcacg aaatattcaa catgctgctg
    26701 gcggtcttcg atcgggcagc gttaatgctt atctgcctgt tctttctcat ccgtatccgc
    26761 ctgtttcgcg aactgttgca caagtcggca cactcaccga aagagttgct cgccgttacc
    26821 gccattttct cgctgttcgc cctcttcagc acctggtccg gcgttcccgt agaaggctcg
    26881 ctggtgaacg tacgtattat cgcggtgatg tccggcggga ttctgtttgg cccgtgggtg
    26941 ggcatcatta ccggcgtgat tgcgggtatt catcgttatt taattgatat cggtggcgtg
    27001 acggcgatcc cctgctttat caccagcatt ctggctggtt gtatatcggg ctggatcaac
    27061 ctgaaaatcc ccaaagcaca gcgctggcgc gtcggtattc tcggcggcat gttgtgtgaa
    27121 acgttgacca tgattctggt gattgtctgg gcaccaacta ccgcgttggg gatcgatatc
    27181 gtctctaaaa tcgggattcc aatgatcctc ggcagcgtct gtatcggctt tattgtgctt
    27241 ctagtgcaaa gcgttgaggg cgaaaaagag gccagcgccg cgcggcaggc caagttggcg
    27301 ctggatatcg ccaacaaaac gctacctctg tttcgccatg tcaatagcga gtcattacgc
    27361 aaagtttgtg aaattattcg cgatgacatt cacgccgatg cggtggcgat taccaatacc
    27421 gatcatgtgc tggcctatgt tggtgtgggt gaacataact atcagaatgg cgatgacttc
    27481 attagcccga ctacccgtca ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat
    27541 gaagcccacc gcacaccaga gattcattcc atgctggtga tcccattgtg ggagaaaggc
    27601 gttgtgaccg gaacgctgaa aatttactac tgccacgcgc atcagataac ctcgtcatta
    27661 caggaaatgg cggttggtct gtcgcaaatc atctccacgc aactggaggt ttcacgcgcc
    27721 gagcagctac gtgaaatggc aaataaggca gagcttcgcg ccctgcaaag caaaattaat
    27781 ccccatttcc tgtttaacgc tttgaacgct atttcatcgt cgatccgtct gaatccggat
    27841 accgctcgcc agctgatctt taatctgtcg cgttatctgc gctataacat tgaattaaaa
    27901 gacgatgagc aaatcgatat caaaaaagag ctgtatcaaa ttaaagacta tattgccatt
    27961 gagcaggccc gctttggcga caagctgacg gttatctatg atattgatga agaggtgaat
    28021 tgctgcattc ccagcctgct gatccagccg ttggtggaga atgccattgt ccacggtatc
    28081 cagccttgca aaggtaaagg cgttgtcacc atcagcgttg cagagtgcgg caatcgggta
    28141 cgcattgcgg tgcgggatac cgggcacggc atcgatccaa aggtgattga gcgggtcgaa
    28201 gcgaatgaaa tgccgggcaa taaaattggc ctgctgaatg tccatcatcg cgtgaagtta
    28261 ttgtatggcg aggggctgca tatccgccgc ctggaaccag gaacggaaat tgcgttttac
    28321 attcctaacc aacgcacccc agtcgcctca caggctacgt tattgctttg agccggagtg
    28381 atattgtgaa agtcatcatt gttgaagacg aattcctggc acaacaggaa ctgagctggc
    28441 taattaaaga gcacagccag atggagattg tcggcacctt tgacgacggt ctggacgtgt
    28501 tgaagttttt gcagcataac cgcgtcgacg ccatttttct ggatatcaat attccgtcgc
    28561 tggatggtgt gttgctggca caaaacatca gccagttcgc ccataaaccg tttattgtgt
    28621 tcatcaccgc gtggaaagaa catgcggtag aagcgtttga actggaggcg tttgactaca
    28681 ttctaaaacc gtatcaggag tcacgcatta ccgggatgct gcaaaaactg gaagcagcct
    28741 ggcaacaaca gcagaccagc agcacgcctg ccgcgacggt aacgcgtgag aatgacacca
    28801 ttaatctggt gaaagatgag cgaataatcg tcacgccaat taacgatatc tattacgccg
    28861 aagcgcacga gaaaatgacc tttgtttata cgcggcgtga atcctacgta atgccgatga
    28921 acattaccga attttgcagc aaactgccgc cgtcgcattt tttccgctgc catcgctcat
    28981 tttgtgtcaa tctgaacaaa atccgcgaaa tcgaaccgtg gtttaataac acctatattc
    29041 tgcggctgaa agatctggat tttgaagtgc cggtcagccg cagcaaagtt aaagaattcc
    29101 gccagttaat gcatctttaa tcgggaactt tcatgaaagc accaggcttg cccgccgatc
    29161 aacaattttt cgccgatctg ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg
    29221 tctggtttgc cagccagcct gcctcgttgc cggtgggcag tttatgtatt gattttcccc
    29281 gtctggatat cgtgctgcgc ggcgaatacg gcaatctgct ggaagcaaag cagcaacgtt
    29341 tggtggaagg agaaatgctg tttattccgg cgcgcgcggc taatttaccg gtcaacaaca
    29401 aaccggtgat gctgttaagc ctggtgttcg ctccgacctg gcttgggtta tcgttttacg
    29461 atagccgcac cacgtcattg ttgcatcctg ctcgccagat ccagcttccc agcctgcaac
    29521 gcggtgaagg tgaagcgatg cttaccgccc tcacccatct tagccgttcg ccgctggagc
    29581 aaaatatcat tcagccgctg gtgttaagtt tgctgcatct ttgccgtaac gtggtgaata
    29641 tgccgccggg caattcgcag ccgcgcggcg attttctcta tcacagcatt tgtaactggg
    29701 ttcaggataa ttatgcccag ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca
    29761 cgcccaatca tctgtcaaaa ctgtttgctc agcatggaac gatgcgtttt atcgagtatg
    29821 tgcgttgggt gcgaatggcg aaggcgagga tgattttgca gaaatatcat ttgtcaattc
    29881 atgaagtggc acagcgttgc ggttttccgg atagcgacta tttttgtcgc gttttccggc
    29941 gtcagtttgg tctgacgccg ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg
    30001 ttaattctgt ttccagcagc gtcaggatac tttgtgcatc ttgcgcggca aacaacgact
    30061 ggcggaagtt tttattcacc agtttacgcg ccagctgcga gaagactttc acatgattca
    30121 tcccttcgtt agcacccagc gtcagcatga tcaccagttc gacttcgccc atttctgact
    30181 gccagtcaac cggtttcgcc agccgggcaa tgctgatact ggaatgacgg atccactgag
    30241 atttggtgtg cgggatcgcc acgccaaaac caacgccagt ggtgacaatc tcttcccgct
    30301 gccagacatc ctcttccagc tcgaacggat gttcagtgcg cccgttaacg ccgaggttac
    30361 cgcacaggaa ctggatcgcc tgctctttat tgctaaaggc ctgatcaacg aagatattct
    30421 ccagcgccag cagcgggcga acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa
    30481 tttcctgcgc gctgcgacat tcacacgcct gacgtgccag ttcccgacac gcctcgctat
    30541 ccagttgacg aagctggctt ttcaccgccg gaatgcgcgg gctactcata ctcagctcat
    30601 ccaggcccag cccaagcagt agcggcagat aacggctttc accgcccagt tcaccgcaaa
    30661 tgcctaccca tttgccccgc tgatgcgcag tggtaactat ttgctgcaac atgcgcagga
    30721 acgatggcgt aatcgggtta tatagcggcg atacgcgcgg gttattacga tcgaccgcat
    30781 acagatactg ggtcatatcg ttggagccga tactgaagaa atcgacctca tcgcagaagt
    30841 gatcgatgat gtagcacacc gacggcactt ccaccatgat acccagcgta atcgtctctg
    30901 catgacgcag gccatcgcgc ttaagctcaa cgatcgcttt ttggatctcg cccttcaccc
    30961 ataagatctg atcgaggccg tggaccatcg ggatcatcaa ctgggcgttg ccgaaactgg
    31021 cggcgcgcaa aatggcccgc agttgagtgc ggaacaggcc agcaaattcc gggtaaatac
    31081 gtaccgcgcg atagccgagg aacgggttct cttcctgggg aatattcaga taaggaatgc
    31141 ttttatcgcc tccgatgtcc attgtgcgga agataatcgg cttgtcgccc gctgccagta
    31201 gcacctgctg gtaggcttca aattgctcct gctcgtccgg ctcgctgtcg cgatccatat
    31261 agagcatttc ggtacggaac agcccgacgc cttccgcgcc gttggcaaac gcgccaggcg
    31321 cttccagagc ggtgccaata ttcgccgcga tatcaatacg cttgttatca cgggaataag
    31381 ccagctgcgc ggcagcctgc gcctgttgtt tttggcgttt atccgccagc gtctgcgcga
    31441 cctgataata accgctcacc gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt
    31501 caagcactgc aggttgcccg gcataacgcg cgatcgcatc cagcggcagg ccgctcagta
    31561 ccgggatcgc cgaggcgcgg gccagaatca gggtatgcga ggtgcgcccg gttttctcca
    31621 gaatcatgcc cgcaagattt tttaaatcca ggcttaaaaa ctggcttggg gttaaatcct
    31681 cggccaccag aatagtcggt ttttccagca ccagattgtt gcgcggcttc agttccggcc
    31741 aggtaatatg cagcaactgt tcgctaatat cgcgaatgtc gctaacacgt tcgcgcagat
    31801 aatcgctggc agaagcagaa agtttggcgc aaacctgctc catatttctg atgatcgccg
    31861 cccccagccc ctgatgctgt tctgtcatca ggcgacggat attgcctgca aattcatcat
    31921 cctgaatcag cgacaaatgg gcgctgagga tagttttgct ttcgccgtca cgctcacgca
    31981 attgctggtt cagttgctcg gcaagcgttg ccaggctgtg ctccagccgg gtggaatctt
    32041 gcgcactggc ggggattgcc cgataactgt cgaggctgtc gctttgtaac agggtcagcg
    32101 tacccacgcc gacgccgctt gccagcacat tgccgtacag taaatccggg ttaaggcggc
    32161 ttaatgaacg cggcagcgga tgcgccgtca gttctgcctg cgtaggctga acgctgtcgc
    32221 tgtcgataaa gcgcacctgg atgtactctt ccagcacgcg ccgcgcctgc tcttcatcgc
    32281 tgccgctaat gttcaggctg caactgtcat taaatagggt gccggtgcca atcagcgcca
    32341 gcgagctttt ggcatcggcc tttgcgttct ggcgatggtt aataaaagtg atttcgcttt
    32401 gccactggct gcactgttct ttaagttccc acgccggacg agcatgtaga ccgttaggca
    32461 aaggacagag aaattgaatc gttaacataa ccgctccggg ttaatcagga gatcatctga
    32521 aatccgtcag ttgaacaacc gtctcacgcg taagacgttg aataagggca gataaaagtt
    32581 gctccatctg caaaatgtca cggcaatcgg caatcgacgc ggcgcaatgt ccgtggcggg
    32641 tggcaggccc catcaccact gtgggtacgc cagtaccggt taagtgcacc gctccaccgt
    32701 ccgtgccgcc gttactgaac atatccgcct gtaacggcac gccaatttct gccgccacgg
    32761 tttcgatcca ggcggtgagt tttggcggcg caatcagtga cttgtcgctt aacaccagca
    32821 tcgggccgtt accaatctgg cgatggttag ccgcgccata attaaagttt ttcgcccagc
    32881 aggcggtatc aaggacaatg gcgacgtccg gcgacaccgc gcgggtggca gtttgcccgc
    32941 cgcgtaaccc cacctcttcg ctggaactgg ccaccagcca cacttccgca ggcagttcag
    33001 cgctgtgtag ttcgcgcagt aacgtcacca gcaggtagca accgaggcgg tcatcaaagg
    33061 ctttccccat cacccgctgg tgggggagaa cctgaaaagt ggtatcgaac gtgacgcgat
    33121 cgcctggacg aattcccgcc tgcatcactt cgtcatacga gcgcgcgcca atatccacgc
    33181 gcatggcgct gacatcattc ccctgccggt cgccgtcaag caggcctgga attttgcact
    33241 cttcacgggt ggtgatgcgc accggctgca gctggcgggc agccatgcgt acattgccaa
    33301 ccggcagcac atcaatcgcc ccttcgcggg agatgctgcg caccataaat cccacttcgt
    33361 ccatatgcgc acagatcatc accttcggac ctgtcgattc attgaggcgg atcagcaccg
    33421 atcccagccc atcaaatcgc acttcttttt gcaggcgatc cgcttcttcc agcaggatct
    33481 gccgcacttc ctgttccgag gaggcgatcg catctgcctc gctcaacgct ttcaatagcg
    33541 ataaatccat tatgcctctc ccgtgagcaa cactgttttc ggcatggcgt agagcacttc
    33601 tgcgccttgc ggggtgacca gcacaacatc ctcgatgcgc acgccccctt gccctggcaa
    33661 ataaatcccc ggctccacgg tcagtaacat gcctggctgt agcgtcgtgg tgtcccgcgg
    33721 tgaaaaacgc ggatcttcat gaacttcaat gccgatagca tgagcggtgt tatgaccgaa
    33781 atagtggcta aaacctgcct cggtaatcac ccgacgcgcg gcttcgtcaa cctgctggca
    33841 gcgcacgccg gggcgaattg cagagattgc tgcgagctgt gcctgcaaaa caatctgata
    33901 gacgttaaac agcgggtgag attcggcgct caccccttcg ccattcacca gcaaggtgcg
    33961 cgtcatatca gagcagtagc cctgatagag cgcaccgaaa tcgagagtga caaactcgcc
    34021 cgctgcaaca atcttgtcgc tggctttgcc gtgcggcagc gccccacgcc agccactggc
    34081 gacaatggtg tcaaaagagg ttttttctgc gccctgctgg cgcataaacc actccagttc
    34141 agcggctatc tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat gctctgcacc
    34201 gcgatcggca atcccacagg caaggcggat tttctccacc tcctctggcg ttttgatttg
    34261 ccgcagcaca tccggcgtgg cgctaaccag tttcgcattg agttcagact gccagcgatg
    34321 cgcggtttcc caactcacct gctggccttc aaaaccgagc gtttgcaact gctcatcggc
    34381 aatgatttgc ctggcgatgg ttgtaagcgt gtgcgtcgcg tcaagcaaat gcagctggta
    34441 accttgcgtg cgggcttcta catccgcgta atagcgcgag tccaccagaa tgtgcgcact
    34501 ttcacggcta atcagcacat agcctgatcc ggtggagatc cccagatgcg gctgtttgtt
    34561 ctgccgtgag gagagaagca ccgcatccag ttgttgcgcc ttaagccagt cgcgcagcga
    34621 agcgagtaat gtcatctgga ttttccttct tacaggctat cgattaacaa tttgccttta
    34681 cggaacatca tcagacgcag gaacaccacc atcagcgcag ttatgactgc ccccagcgcg
    34741 atccccgcca tatagacgcc caggttagtg accagcggcc atgcccagat agcggattcc
    34801 gggaaccatt gcactgcgcc cagccagaca gcggcggttg agccgacaat cgcgccgacc
    34861 atatacgacg gaatggcggt gatggggctt tccagcgcaa acggaatcgc accctcactg
    34921 atccccataa aggcaaggaa catcgcggtc ttaccctgcg gataaagttg tgcgttgaac
    34981 aggcgtttgc cggttaaacg acggtcaata atggtcgcca gacccagacc aatcggcgga
    35041 ataacgatag cgatagaacg agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag
    35101 gcaacaaaac ctgcggcttt gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg
    35161 atccccattg cgtacatcag cgcgcccttc tcacctgcgg cagtcagcac agtacggata
    35221 ccgccgttga tccagccccc aaagggggtg atgacgtagt acatcgccag catgacaaaa
    35281 atagcggaaa gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa atccggtaac
    35341 tggattttct ggttcatcca tttcacaagg taaccggcaa cgatagaaat aatcagcgca
    35401 ccaatgaagg tggacggcac cggcgaagag gtcgcccatt gcatagtgct gggatcgaag
    35461 ttcagcagtt gcgtcggctg ggtagacatc aagccgccga taaaaccagc cggaaacgcc
    35521 agtttgccac cgatagagtt cgccacaaaa gcagcaaaca tcggaatggc aaaaccaaac
    35581 aacacgccgc cgaaggactg cgacaaccag gcaaatttca gtaaggagag gtcgaaaccg
    35641 gagaatttcc cgctattaag agcatccatg atgccgatct cagcaggaat tttaagccag
    35701 ctataagcaa tcaactggct gaaagcgagg atcacaccgc ccataattaa ggtcggcacc
    35761 atgcgtgaaa tccctgacat cacatgctgc gggagttcac cccagaagct gcttttagag
    35821 gcctgcggat tcttaactgc cgctgccgca ccggatgcgc caggcacaac ggttgcactg
    35881 cgttttttaa tggccataac aattatccct gtcgaatatt actgttgttc agaggcaatc
    35941 atctcttcga tttctttgat gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt
    36001 tcataaacgt cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc cacggagtgg
    36061 atgatgatgg tcgcttcggc gatatcctgc gccgtcaggc gattctggat accgtccgcg
    36121 ccctgggttt caattttcac ttcataaccg gcttctaccg ccgcttcttc cagcgcctga
    36181 gcggccataa aagtgtgagc caggcccatt gggcaggcac ataaggcaat cagtttctta
    36241 ctcatcgtta attcctcgtt cagtgatagt tgacgagtga aattatgaac gtgcaaaaca
    36301 aatcgccgtt accagacaaa taatgcattt actggagaat taatcccgct gtacggaagt
    36361 gtgacgcagg tcggccttgt gaggggacac aaagaaaagt tgaattaact gtatgattta
    36421 aaagattatc gggagagtta cctcccgata taacaggaag gatttacaga atgtgaccta
    36481 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    36541 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    36601 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgcccgca ataaacacgc
    36661 cgccaaatgt cccgagattg agcgccagat tgccgccaaa acggcccata atgacgcaaa
    36721 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    36781 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    36841 tcaccagtcc tgggccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    36901 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    36961 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccccg
    37021 ttccggcacc gtaaaccgca ataggcttac cttctaccgg ttctgcgcca ccaaactgaa
    37081 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    37141 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    37201 ggttggtcat tgccacccag tctccggtaa tcgggcaagc gatggcaata cagccgtctt
    37261 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    37321 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    37381 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    37441 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    37501 gccggaaagg caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc
    37561 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    37621 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    37681 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    37741 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcaccc
    37801 ctctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    37861 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accggttccg
    37921 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    37981 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgcggttgc cattggcgct
    38041 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    38101 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    38161 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    38221 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    38281 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    38341 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat
    38401 ccggtgctcg tgctgggtat tggcggattt attctcggta ttctgggggt tattggtgga
    38461 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    38521 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    38581 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    38641 ttgatgctgc atgagcacgt tcccgccgta ccagcggcaa taaccgtttc ctgcgctatt
    38701 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    38761 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    38821 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    38881 aacgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    38941 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    39001 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    39061 aacgcaggcg aaatcaccct actgccatca atcaaattac aaattggcga tcgcgatcat
    39121 tacggtaatt actgggacgg tggtcactgg cgcgaccgtg actactggca tcgcaattat
    39181 gaatggcgta aaaaccgctg gtggcgtcat gataatggct accatcgtgg ctgggataag
    39241 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    39301 aaaggccgcg gacatggaca ccgccattaa acacattcgc caatggagca caaggcctga
    39361 tgcgacgcta gtgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    39421 aggcgtttac gccgcatcgc caatagtgcc agatgcgacg ctcattcaac tacaatccca
    39481 gcgccgtccc caccaataac cagatattca gcgccacgac cagcactaca atcacccagc
    39541 ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga
    39601 aaatcagcag tggaaccagc gccagggcga taccaaaact taacagcacc tgactcataa
    39661 ccagaatccg tgtcggatct aatcccatca gaatgacaat aaatgacggc agcatggtga
    39721 ctgtacgacg cacccacagc gggatatgga agcgaatgaa cccctgcatc accacctgcc
    39781 ctgccagtgt ccccaccacc gttgaggaca gtccggcagc aaccagactt aacccaaaga
    39841 ccgttgccgc agcatggctt aacagcggtt gcagcgtcaa ataagcctca tcaagatcgg
    39901 caacaccagt atgaccagaa aagtggaacg ccgccgcagc tgtagccatc atcgccagat
    39961 tgacaaaacc ggcaatagtc atggcgatag ccacatccca tttggtggcg gaataacgtt
    40021 gttgacgcga accgccatgt aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg
    40081 gcataatcgt cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac
    40141 tcgggatcac cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga
    40201 caatgtaagc cgcggcaaca aacaacagta acccgccaat cactttctcc agcggttttt
    40261 gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct
    40321 gcaacaacga aacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca
    40381 ggtcggttgc catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg
    40441 gatagtgatc gcgaatctgc tccgccagat ttttaccggt ggcaatccct agtttggcag
    40501 agaggatctg aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat
    40561 agccgaagct ggcacccgcc tgaatattgg tcgcaaagtt accgggatcg atataaccaa
    40621 tcgccgcaat gaacgcaggt cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc
    40681 tgctactctc aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt
    40741 ttcatgctat gccaaacgag aatgattatc aaattcattt aaatgggttg tggtgatttc
    40801 tctgatagac caggattatg actacgaaaa gattgatgac ttgaatgtta tggttatgtt
    40861 taatgttagc acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga
    40921 cagctcacta tttttgaagc ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt
    40981 ttccttaacc atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca
    41041 aatatggacc gcgtccttca ttttgtactg gcacttgccg ttgttgcgat cctcgcactg
    41101 ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc
    41161 gaagtgttac tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc
    41221 ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg aagggactaa cttcgtcttt
    41281 ggtagcatga atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc
    41341 tttatctctg cactgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc
    41401 gcaattggtt tcctgctctc caaagtcaac ggcatgggca aactggaatc ctttaacgcc
    41461 gtcagctccc tgattctggg tcagtctgaa aactttattg cctataaaga tatccttggc
    41521 aaaatctccc gtaaccgtat gtacaccatg gcagcaacgg cgatgtccac cgtttctatg
    41581 tccatcgttg gtgcatacat gaccatgctg gaaccaaaat acgtcgttgc agcgctggta
    41641 ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca acccttaccg tgttgatgcc
    41701 tgtgaagaaa acattcagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg
    41761 ggtgaataca ttctggcagg tttcaaagtt gccattatcg ttgccgcaat gctgattggc
    41821 tttatcgccc tgatcgccgc gctgaacgca ctgtttgcta ccgtgactgg ctggattggc
    41881 tacagcatct ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg
    41941 ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc
    42001 aacgagttcg ttgcgatgat ggatcttcag aaaattgcct ctacactctc tccgcgtgct
    42061 gaaggcatca tctctgtgtt cctggtttcc ttcgctaact tctcttcaat cgggattatc
    42121 gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg
    42181 aagctggttt acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg
    42241 ctgtaagacc atacataaaa aagccgggga taattccccg gcttttttac gcctgttaat
    42301 cagctaatgg ctgcgggcga ccaatcaaat acccttgcag atattgcacc ccgagcttat
    42361 gcaatagcgc ctgttgctgt tgcgtctcga caaactccgc gaccacactc aatgacttcg
    42421 ctttcgccag atcggtaatt gatctcacaa tcatcgcatc cagcgtattc gtgacaatat
    42481 ctttcacaaa gacgccatca attttgatga tatcagcctg caaacgcttt aaccgttcgt
    42541 agttggcata gccggtgcca aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct
    42601 gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa
    42661 gaatgaccgc ctgcggggag atgtgataac gcttaaatag acgaataatc cgcccggcaa
    42721 tattcttttg cagcagcgtg agcggcatta aattaactga aaagcgcggg ccttttttgt
    42781 cgcaagggtg tgttgccagc cacttcaaca aggattccag cacttgcaaa tcaaaacgcg
    42841 cgctaaggtt aaactgagca ataaggggca gaaacttatc cggggtcata atgccgccgt
    42901 catatttcag tcgcgcgagg acctcatcat aaccttcacc ctctttgttg cgaattggct
    42961 gggcgtagag cagcaaatca ccctgatcta acgccgtgcg aatggtattc agcaatagca
    43021 cctgtttagt ggtctgcccg gaaaccatct cctctcggct atccagcgcc agcacatgat
    43081 gatgtgcgca ggattgctcc gccagccagc ttaactgccc caacaagggt tgcagggttt
    43141 cctgatttcc atcaaaacgc ccccaggcag caccatagcc catatccagc ccggtattgt
    43201 tccagtgaat ttgccgacta ttcaggatgt taaccatatg ctggagtcgc ccttccgttt
    43261 caggcccgct cagcactaac agcagttcac tacccggcaa ttgatacaac ttttcgtttt
    43321 cctgcatcaa tggcagcagc gtacggtaaa ttgatcggat acagtgaaca cgcatcatca
    43381 acccgtaatg acgactcata aactcaagat tatcaatgcg caggcagcaa aaactcttgc
    43441 ccgcctcttg ctccggcgct tgttccaacg cacgaaagtt gggcaggagc gttaacggat
    43501 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    43561 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat
    43621 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagcaggcaa agcgttgaaa
    43681 cagcccaagt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    43741 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    43801 gaagtagcag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    43861 ccaacgacgg agcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    43921 tgcgagtgag atagtagaaa agcatgttgt aaatcagcac agcggtgaaa aggcttagca
    43981 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    44041 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    44101 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    44161 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    44221 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    44281 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    44341 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    44401 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    44461 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    44521 acacaaataa taagtttatt cgcattgcga agcaaacagg ggccatcctg ttgccgattt
    44581 ttcatccaat gatgctttca ccaggcggca ctaacccgga cgtagactgt attacaaaag
    44641 cggcaaaaag cagagacaaa aaacccccgc tttgcagcga gggttggaaa tttggtggag
    44701 ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct
    44761 atagccccac gatgcgttta cgtaccaaat ttgctgggtg caaaatttgg tggagctaag
    44821 cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc
    44881 cccgtcacgt aaagcttgtc gagttgacgg gcggcatcat atgaattccg cccgaatgtg
    44941 tcaacggcaa attgcaacgt gtagtttcaa tcgctgaaaa atcaggcaaa tgaacaattt
    45001 tggaaaaccg ctcgcattca gtagttattc atgtcacggt ttcctgtaaa gtggtgctat
    45061 aaaatgaact actaatagac ccacatacat tcagggaatt gttatgttca aggagaggat
    45121 gacaccagat gaacttgcca gactgaccgg ctatagccgc cagaccatta ataaatgggt
    45181 acgcaaggaa ggctggacaa cgtcaccaaa acctggcgtc cagggtggca aagcccgact
    45241 ggttcacgtc aatgaacagg ttcgtgaata tattcgtaat gctgaacgtc cagaaggtca
    45301 gggagaagcg cctgcccttt ctggtgatgc gccgcttgaa gtattgctgg tgacactggc
    45361 gaaagagatg acgccagttg aacaaaaaca gtttacatcc ttgcttctgc gggaagggat
    45421 tatcggattg ttacaacgct tagggattcg cgatagcaaa taatatgaaa agattacgca
    45481 ataaaatgac caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa actgttaacc
    45541 gttggatcag agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa ggcggtcgtg
    45601 ccagacttat tctggtcgat acgcaagttt gcgagtttat tcagaacacg ccagccttcc
    45661 ataacacacc gatgcttctg gaagccgaag aaccccttgc cgaatacgca ccggggattc
    45721 gcactcctgc ttatcggcaa atcatcagtg caatagacaa catgactcac agtgagcagg
    45781 aaaaagttgc gcaatttttg tcacgcgaag gaattcgtaa tttccttgct cgtctcgaca
    45841 tagatgaatc agcttgacat cctccacgcc ctgaatgacg aggatccctg ctacgttcag
    45901 gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc
    45961 tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc gtttacatcg
    46021 gcgttcgctg tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttttct
    46081 ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg aacagccag
//
LOCUS       DAZFKY010000015        45528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFKY010000015 DAZFKY010000000
VERSION     DAZFKY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..735)
                     /locus_tag="ACHKCL_000760"
     CDS             complement(<1..735)
                     /locus_tag="ACHKCL_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5761795.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANI"
     gene            743..1168
                     /gene="tnpA"
                     /locus_tag="ACHKCL_000761"
                     /pseudo
     CDS             743..1168
                     /gene="tnpA"
                     /locus_tag="ACHKCL_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1286..2701)
                     /gene="gltX"
                     /locus_tag="ACHKCL_000762"
     CDS             complement(1286..2701)
                     /gene="gltX"
                     /locus_tag="ACHKCL_000762"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5761796.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            2960..3035
                     /locus_tag="ACHKCL_000763"
     tRNA            2960..3035
                     /locus_tag="ACHKCL_000763"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2993..2995,aa:Val,seq:tac)
     gene            3080..3155
                     /locus_tag="ACHKCL_000764"
     tRNA            3080..3155
                     /locus_tag="ACHKCL_000764"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3113..3115,aa:Val,seq:tac)
     gene            3202..3277
                     /locus_tag="ACHKCL_000765"
     tRNA            3202..3277
                     /locus_tag="ACHKCL_000765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3235..3237,aa:Val,seq:tac)
     gene            3282..3357
                     /locus_tag="ACHKCL_000766"
     tRNA            3282..3357
                     /locus_tag="ACHKCL_000766"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3315..3317,aa:Lys,seq:ttt)
     gene            3477..3818
                     /locus_tag="ACHKCL_000767"
     CDS             3477..3818
                     /locus_tag="ACHKCL_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO5761797.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(3809..4735)
                     /gene="yfeR"
                     /locus_tag="ACHKCL_000768"
                     /pseudo
     CDS             complement(3809..4735)
                     /gene="yfeR"
                     /locus_tag="ACHKCL_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4825..5823
                     /gene="yfeH"
                     /locus_tag="ACHKCL_000769"
     CDS             4825..5823
                     /gene="yfeH"
                     /locus_tag="ACHKCL_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5761798.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5820..6038)
                     /gene="ypeB"
                     /locus_tag="ACHKCL_000770"
     CDS             complement(5820..6038)
                     /gene="ypeB"
                     /locus_tag="ACHKCL_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5761799.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6040..8055)
                     /gene="ligA"
                     /locus_tag="ACHKCL_000771"
     CDS             complement(6040..8055)
                     /gene="ligA"
                     /locus_tag="ACHKCL_000771"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5761800.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8126..9124)
                     /gene="zipA"
                     /locus_tag="ACHKCL_000772"
     CDS             complement(8126..9124)
                     /gene="zipA"
                     /locus_tag="ACHKCL_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5761801.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9354..10115
                     /gene="cysZ"
                     /locus_tag="ACHKCL_000773"
     CDS             9354..10115
                     /gene="cysZ"
                     /locus_tag="ACHKCL_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5761802.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10300..11271
                     /gene="cysK"
                     /locus_tag="ACHKCL_000774"
     CDS             10300..11271
                     /gene="cysK"
                     /locus_tag="ACHKCL_000774"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5761803.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11655..11912
                     /gene="ptsH"
                     /locus_tag="ACHKCL_000775"
     CDS             11655..11912
                     /gene="ptsH"
                     /locus_tag="ACHKCL_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5761804.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11957..13684
                     /gene="ptsI"
                     /locus_tag="ACHKCL_000776"
     CDS             11957..13684
                     /gene="ptsI"
                     /locus_tag="ACHKCL_000776"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5761805.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13725..14234
                     /gene="crr"
                     /locus_tag="ACHKCL_000777"
     CDS             13725..14234
                     /gene="crr"
                     /locus_tag="ACHKCL_000777"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5761806.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPQLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14277..15128)
                     /gene="pdxK"
                     /locus_tag="ACHKCL_000778"
                     /gene_synonym="thiJ"
     CDS             complement(14277..15128)
                     /gene="pdxK"
                     /locus_tag="ACHKCL_000778"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5761807.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15233..15600
                     /gene="yfeK"
                     /locus_tag="ACHKCL_000779"
                     /pseudo
     CDS             15233..15600
                     /gene="yfeK"
                     /locus_tag="ACHKCL_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(15603..16514)
                     /gene="cysM"
                     /locus_tag="ACHKCL_000780"
     CDS             complement(15603..16514)
                     /gene="cysM"
                     /locus_tag="ACHKCL_000780"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5761808.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16648..17745)
                     /gene="cysA"
                     /locus_tag="ACHKCL_000781"
     CDS             complement(16648..17745)
                     /gene="cysA"
                     /locus_tag="ACHKCL_000781"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5761809.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(17735..18610)
                     /gene="cysW"
                     /locus_tag="ACHKCL_000782"
     CDS             complement(17735..18610)
                     /gene="cysW"
                     /locus_tag="ACHKCL_000782"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5761810.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(18610..19443)
                     /gene="cysT"
                     /locus_tag="ACHKCL_000783"
     CDS             complement(18610..19443)
                     /gene="cysT"
                     /locus_tag="ACHKCL_000783"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5761811.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(19443..20459)
                     /gene="cysP"
                     /locus_tag="ACHKCL_000784"
     CDS             complement(19443..20459)
                     /gene="cysP"
                     /locus_tag="ACHKCL_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5761812.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(20617..21408)
                     /gene="ucpA"
                     /locus_tag="ACHKCL_000785"
     CDS             complement(20617..21408)
                     /gene="ucpA"
                     /locus_tag="ACHKCL_000785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5761813.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21537..22394)
                     /gene="murR"
                     /locus_tag="ACHKCL_000786"
     CDS             complement(21537..22394)
                     /gene="murR"
                     /locus_tag="ACHKCL_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO5761814.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            22558..23454
                     /gene="murQ"
                     /locus_tag="ACHKCL_000787"
     CDS             22558..23454
                     /gene="murQ"
                     /locus_tag="ACHKCL_000787"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5761815.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23458..24882
                     /gene="murP"
                     /locus_tag="ACHKCL_000788"
     CDS             23458..24882
                     /gene="murP"
                     /locus_tag="ACHKCL_000788"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5761816.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24887..26191
                     /gene="pbp4b"
                     /locus_tag="ACHKCL_000789"
     CDS             24887..26191
                     /gene="pbp4b"
                     /locus_tag="ACHKCL_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO5761817.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(26249..27148)
                     /gene="yfeX"
                     /locus_tag="ACHKCL_000790"
     CDS             complement(26249..27148)
                     /gene="yfeX"
                     /locus_tag="ACHKCL_000790"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5761818.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(27244..27819)
                     /gene="yfeY"
                     /locus_tag="ACHKCL_000791"
     CDS             complement(27244..27819)
                     /gene="yfeY"
                     /locus_tag="ACHKCL_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5761819.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27880..28329)
                     /gene="yfeZ"
                     /locus_tag="ACHKCL_000792"
     CDS             complement(27880..28329)
                     /gene="yfeZ"
                     /locus_tag="ACHKCL_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5761820.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(28316..28741)
                     /gene="ypeA"
                     /locus_tag="ACHKCL_000793"
     CDS             complement(28316..28741)
                     /gene="ypeA"
                     /locus_tag="ACHKCL_000793"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5761821.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28955..29824
                     /gene="amiA"
                     /locus_tag="ACHKCL_000794"
     CDS             28955..29824
                     /gene="amiA"
                     /locus_tag="ACHKCL_000794"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5761822.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29828..30727
                     /gene="hemF"
                     /locus_tag="ACHKCL_000795"
     CDS             29828..30727
                     /gene="hemF"
                     /locus_tag="ACHKCL_000795"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5761823.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30733..31785)
                     /gene="eutR"
                     /locus_tag="ACHKCL_000796"
     CDS             complement(30733..31785)
                     /gene="eutR"
                     /locus_tag="ACHKCL_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5761824.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31831..32331)
                     /gene="eutK"
                     /locus_tag="ACHKCL_000797"
     CDS             complement(31831..32331)
                     /gene="eutK"
                     /locus_tag="ACHKCL_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5761825.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32344..33003)
                     /gene="eutL"
                     /locus_tag="ACHKCL_000798"
     CDS             complement(32344..33003)
                     /gene="eutL"
                     /locus_tag="ACHKCL_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5761826.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(33013..33900)
                     /gene="eutC"
                     /locus_tag="ACHKCL_000799"
     CDS             complement(33013..33900)
                     /gene="eutC"
                     /locus_tag="ACHKCL_000799"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5761827.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33921..35282)
                     /gene="eutB"
                     /locus_tag="ACHKCL_000800"
     CDS             complement(33921..35282)
                     /gene="eutB"
                     /locus_tag="ACHKCL_000800"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5761828.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(35294..36697)
                     /gene="eutA"
                     /locus_tag="ACHKCL_000801"
     CDS             complement(35294..36697)
                     /gene="eutA"
                     /locus_tag="ACHKCL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5761829.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36694..37920)
                     /gene="eutH"
                     /locus_tag="ACHKCL_000802"
     CDS             complement(36694..37920)
                     /gene="eutH"
                     /locus_tag="ACHKCL_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5761830.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(38020..39207)
                     /gene="eutG"
                     /locus_tag="ACHKCL_000803"
     CDS             complement(38020..39207)
                     /gene="eutG"
                     /locus_tag="ACHKCL_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5761831.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(39197..40033)
                     /gene="eutJ"
                     /locus_tag="ACHKCL_000804"
     CDS             complement(39197..40033)
                     /gene="eutJ"
                     /locus_tag="ACHKCL_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5761832.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(40044..41447)
                     /gene="eutE"
                     /locus_tag="ACHKCL_000805"
     CDS             complement(40044..41447)
                     /gene="eutE"
                     /locus_tag="ACHKCL_000805"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5761833.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41459..41746)
                     /gene="eutN"
                     /locus_tag="ACHKCL_000806"
     CDS             complement(41459..41746)
                     /gene="eutN"
                     /locus_tag="ACHKCL_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5761834.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41853..42146)
                     /gene="eutM"
                     /locus_tag="ACHKCL_000807"
     CDS             complement(41853..42146)
                     /gene="eutM"
                     /locus_tag="ACHKCL_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5761835.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42185..43201)
                     /gene="pta"
                     /locus_tag="ACHKCL_000808"
     CDS             complement(42185..43201)
                     /gene="pta"
                     /locus_tag="ACHKCL_000808"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5761836.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(43198..43929)
                     /gene="eutT"
                     /locus_tag="ACHKCL_000809"
     CDS             complement(43198..43929)
                     /gene="eutT"
                     /locus_tag="ACHKCL_000809"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5761837.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(43926..44627)
                     /gene="eutQ"
                     /locus_tag="ACHKCL_000810"
     CDS             complement(43926..44627)
                     /gene="eutQ"
                     /locus_tag="ACHKCL_000810"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5761838.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44602..45081)
                     /gene="eutP"
                     /locus_tag="ACHKCL_000811"
     CDS             complement(44602..45081)
                     /gene="eutP"
                     /locus_tag="ACHKCL_000811"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5761839.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45094..45429)
                     /gene="eutS"
                     /locus_tag="ACHKCL_000812"
     CDS             complement(45094..45429)
                     /gene="eutS"
                     /locus_tag="ACHKCL_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5761840.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        10513 a        12715 c        11602 g        10698 t
ORIGIN      
        1 gatatttgcg gtacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttatt
       61 gctgaatttg accttgcggc ttaactgtcg ctgaagtctc gccaacttct tctggttttt
      121 ctgaaaactg tttacaggct caaagactgt gccatctgac agcgtggcga gtttagccac
      181 gccagcatcc agtccgacca ttgatgctga agggtgaacc ggagttgata cttcactttc
      241 tgtctgaata ctgatgtacc acttaccgca ggactggctg acagtgacat ttttcacaac
      301 acccgtgacc tgacggctgt tcaggtagcg catccagcca agtttcggca gaaaaatacg
      361 gctgttttcc tgatcgagct taacgccctg cgggtagcgg aatgcatcat tctgtccccg
      421 ctttttgaat cggggaaaag cagcccgctt ccggaagaag tttttgtaag cccgctcaag
      481 ctctttcagt gactgttgca atggctgtga cggggcatct ttaagccatt gcgtttcagt
      541 ggcgtttttc cactcaacca gccaggaagc cattttcccg taagggatgt atttattacc
      601 ggcctcatga ttctcattct gaagcgccag agcacgattg aaaacgaaac gacatgcgcc
      661 tgcgaagcgc ctcatttcac gctcctgttg accacctggt cttaactgga atttaaatgc
      721 ctgtagtcgt ttcacaggcc aaatgatact ttggtctatg aataaagaaa ccgatatccg
      781 acggggaagg cattgtgttt tcctgatgca tgttcatctg ttctttgtca ccagataccg
      841 acgtcagatt tttgactatg acgcgacaga aaaactacgc acttactttt caaatgtatg
      901 tgctgatttt gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatttgtt
      961 aactaactat cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc
     1021 gggtaggtta ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt
     1081 tttgtggagt cctggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
     1141 ccaatacatt gaacaacagc aaacacctgg tcaggtggaa aaccgcgcct tatatccccg
     1201 ccctgaagga cggggtttta cggcgcaccg gataaataaa aaacggcagg ggtatctctc
     1261 ctgccgttta tcttttatgc gctaattact gctgattttc gcgttccgca ataaaagcca
     1321 gcgctttgtt gatacgctcg atactgcggg tcttaccgat cgcgtgaacg gtaacgtcca
     1381 gcgccggaga ctgccccgca ccggttacgg cgacacgcag cggcatacca actttaccca
     1441 tacccacttc cagctcatcc gccgtcgcct gaatagcgtg atgaacgttt tcagctgtcc
     1501 agtcagtaat cgcggccagt ttgtcacgaa ccacttccag cggctgacgc gctaccggac
     1561 gcagatgttt tttcgcggcg tcggcatcga actcagcaaa atcttcgtag aaataacggc
     1621 agctctgtgc catctctttc agcgtcttgc agcgttcgcc cagcagtttc accagatcag
     1681 ccagctgcgg gccgttacgg gtatcgatat tttcctgctc aatgtgccac tgtaagtgag
     1741 tagcaacata ctccggcggc agcgcgttaa tgtagtgatg gttcagccac agcagcttgt
     1801 cggtgttgaa cgcactggca gatttgctga cggcattcaa agtgaagtat ttgatcatct
     1861 cttcacgagt gaagatttcc tgatcgccgt gggaccagcc cagacgcacc agatagttca
     1921 gcagtgcttc tggcaaataa ccgtcatcac gatactgcat tacgctgact gccccgtgac
     1981 gtttggacag ttttttgccg tcatcgccgt tgatcataga aacgtgcgcg taaaccggta
     2041 ccggcgcttt cagggcttta agaatgttga tctggcgtgg cgtgttgttg atatggtctt
     2101 cgccacggat aacgtgggtg atttccatat cccagtcatc gacaaccaca cagaagttat
     2161 aggttgggga accatcggta cggcggataa taagatcgtc cagttcctgg ttgctgaact
     2221 cgatcggacc acggatctga tcgtcaaaaa caacagaacc ttcctgcggg ttagcaaaac
     2281 gtacgacaca cggctcatca tcagcgtgat gctcatgact gtggcggcag cgaccgtcat
     2341 aacgcggctt ctcacctttc gccatttgct cttcgcgcag cgcttccagg cgctctttag
     2401 agcaatagca tttataagct gtgccctctt ccagcatctg atcgatcacc gcgttgtagc
     2461 gatcaaaacg tttggtctgg aagtacggac cttcatccca ctccaggctc agccagttca
     2521 tgccatccat aatggcttcg atagcttccg gcgtggaacg ctcaagatcg gtgtcttcaa
     2581 tacgcagcac gaactcaccg ccgtgattac gtgcaaaaag ccaggagtaa agagcagtac
     2641 gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc gaagcgagtt ttgattttca
     2701 tgaaatggcc ttacgtttag aaagatgccg acaaccggca aatcctggaa aaattgagtg
     2761 ggcgatattc tatcactccc gcctgattcc tcaatgtagt tcgggttgca accttacgca
     2821 ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt tgcgatcatc ttgcttaaat
     2881 ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact cattttgaac tctccctata
     2941 atgcgactcc acacagcggg ggtgattagc tcagctggga gagcacctcc cttacaagga
     3001 gggggtcggc ggttcgatcc cgtcatcacc caccaactac tttatgtagt ctccgccgtg
     3061 tagcaagaaa ttgagaagtg ggtgattagc tcagctggga gagcacctcc cttacaagga
     3121 gggggtcggc ggttcgatcc cgtcatcacc caccactttc tcaccagcta aatttcttgt
     3181 aaaaatgtga agtaccgaag tgggtgatta gctcagctgg gagagcacct cccttacaag
     3241 gagggggtcg gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg
     3301 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
     3361 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat ttgtaagatt gatttttctt
     3421 ctcaagttcc ttgttcttct gccgatatta cctcgtacag tgacgaggac tgaacaatgg
     3481 ccactatctc ttcaacttca attccttcca tacaaacaca gagtagtaac agggcatcac
     3541 aaggtagcga tgttgcttca caaatcgcgc ggataagtca gcaaatcatc aaactcactc
     3601 aacacattaa agagattgta gacactagcg gcagtgctga agacaagcaa aaacaggccg
     3661 aattgattca acagcaaatc accctactgg aaacacaact ggcgcagttg cagagacagc
     3721 aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct gaatgtatcc cttctaaatc
     3781 ctgttgaaaa tacgactcat atcggtatct atatctgata caacggatcc ccttcccgcc
     3841 cagccattag cgtattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg
     3901 aaagtgaacg gtttttccta cgcaccagca tcaactggcg ttccaccacc ggcgtaatgc
     3961 gctttaccac cagcgggcta ccttcaggca atggtagcgc cagtgcggga agaatactga
     4021 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa
     4081 tattcgcctg aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
     4141 cataatcctg caacaccaat ttcgctccct gtaatgcctg ctagggtaca taatcttccg
     4201 cggccaaagc gctatcccga tggcaaagca gaaagaaagg ctcggaaaga atcacttcac
     4261 attgcagatc gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc
     4321 gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga atatctggat
     4381 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
     4441 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
     4501 tgttaagttc atccagcagt cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa
     4561 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagctcct
     4621 tcacactgtg acttactgcc gactggctca ggccgatacg ctctcctgca cgactaaaac
     4681 ttttctcctg cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt
     4741 ttcttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
     4801 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
     4861 accctgatca cggtggtgtt gctggcctct ttctttcctg ccagaggcga tttcgtcccc
     4921 ttctttgaaa atctgaccac cgcagctatt gccctgctgt tctttatgca cggcgcgaag
     4981 ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatg
     5041 tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta
     5101 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg
     5161 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct
     5221 gcgtcagcat ccagtctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
     5281 aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct gcaactgctg
     5341 ctgccgtttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat
     5401 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc
     5461 agcgaagccg tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc
     5521 gtggtggtca gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
     5581 cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
     5641 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
     5701 gtgctgcccc tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt
     5761 cgatacaaac gccagaccga acagttacag gcgcagcagg aaagcagcgc cgataaagct
     5821 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc
     5881 gtgatttgca ttagctcacc gagcttccct gccgggaact catctttgcg ggcaaaccac
     5941 agcagatact cctccggcag gtcgattaag cgacgccctt tgtatttgcc aaacggcatt
     6001 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
     6061 catttccgct tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc
     6121 tgcagcttca cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
     6181 gcccagttcg accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt
     6241 aagcaccacg gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg
     6301 cgcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc
     6361 ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag
     6421 ctcttcaatc gaagcggctt ccagcgcttc cagcgttccg aaatatgccg ccagacctgc
     6481 tgcggtggcc tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt
     6541 ttctttcgct ttttccagcg cgttaaccac gttttgtgcc gattttggcc ccatacgctc
     6601 cagtccggtc agtttgcctg cggtgagttt gaacagatcc gccggagtgt ggacatattc
     6661 tttttcaacc agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
     6721 ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg caaatcagac caccagtaca
     6781 gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatacgt
     6841 cgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg
     6901 cgggatcacg tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
     6961 ttcgatttca tccgcattgt gtaaggttgc gttactcacc agcacgcctg caacatggac
     7021 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
     7081 acgcacaaag gtcatctgtt cctgcgccgg gaatttaaac gctaccgccc agcgcggggc
     7141 tcgcgcgaca aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc
     7201 gtcgatatca aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac
     7261 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcaaccccc actttttaaa
     7321 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc
     7381 atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga
     7441 accagctgcc gcattacgtg ggttagcaaa cactttcccg cccgtgcgtc gcgcatcttc
     7501 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
     7561 cgggatattc tctccgtgca acttcagtgg aatggcgcga atagtacgca cattagacgt
     7621 gatatcttcc ccggtggtgc catcgccacg agtcgcggca ctgactaaaa cgccattttc
     7681 atacagaata ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc
     7741 gttgcttttc agacggtcct gcacacgttt gttgaaggca agaaagcttt cttcatcaaa
     7801 aacgttatcc agcgacagca ttggcacttc atggcgaatc tggctgaaag ccgccagcgg
     7861 cgcagcacct acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
     7921 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
     7981 catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg tcagttgttg
     8041 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
     8101 gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttaacttcg cggatgatgt
     8161 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac
     8221 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
     8281 cgtcaccgta agacggtacc tgcataaaga tagtgacacc cggagtagtg aaatccttca
     8341 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctaaataac gccgggccgc
     8401 tgccatccgg gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt
     8461 gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt
     8521 tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta
     8581 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt
     8641 gcggtgctga atgcactggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca
     8701 acggctgttc aggctgcggc tgataggcag gctgctgcac gggttgctgc accggctgcg
     8761 ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac
     8821 gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga cgagcagcct
     8881 catgctcctg ggcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc
     8941 cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt
     9001 ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta
     9061 ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca
     9121 tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga
     9181 gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat
     9241 gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc
     9301 ggtaaaatac gcacaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt
     9361 catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg
     9421 tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga
     9481 tggggggcgc attctggtgg ctctttacgc aactcgatgt ctggatcccg actctcatga
     9541 gttatgttcc ggactggtta caatggctga gctatctgtt gtggcctctg gctgtcatct
     9601 ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt gccgctccgt
     9661 ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca ccgccagata
     9721 ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat
     9781 ttgcctggta tctgccacgc gcaattgtat tgctaattct ttacttcatc cctggtattg
     9841 ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt
     9901 attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc
     9961 gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagtctgttt acgatgatcc
    10021 cgctgcttaa tctgttcatt atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg
    10081 attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat tttgtaaacc
    10141 gtttgtgtga aacagggggg gcttatgccg ccccttattc catcttgcat gtcattattt
    10201 cccttctgca tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct
    10261 acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat
    10321 aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc
    10381 attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc
    10441 aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa
    10501 ccaaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa
    10561 ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta
    10621 ggtgcaaacc tggtgctgac cgaaggtgct aaaggcatga aaggcgcaat ccaaaaagca
    10681 gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg
    10741 gcaaaccctg aaattcacga aaagaccacc ggtccggaga tctgggaaga taccgacggt
    10801 caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg cgtcagccgc
    10861 tacattaaag gcaccaaagg taagaccgat cttatctctg tcgccgttga gccaaccgat
    10921 tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggccc gcataaaatt
    10981 cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt cgataaagtc
    11041 attggcatca ccaatgaaga agcgatttct accgcgcgtc gtctgatgga agaagaaggt
    11101 attcttgcag gtatctcttc tggagcagct gttgctgcgg cgttgaaact acaagaagat
    11161 gaaagcttta ccaacaagaa tattgtggtt attctaccgt catcgggtga gcgttattta
    11221 agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta atgccagctt
    11281 gttaaaaatg cgtaaaaaag caccttttca ggtgcttttt tgtggcctgc ttcaaacttt
    11341 cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt
    11401 gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt
    11461 caattcttcc tttagcggca taatgtttaa tgacgtacga aacgtcagcg gtcaacaccc
    11521 gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac
    11581 aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa
    11641 gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca
    11701 cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga
    11761 cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga
    11821 ctcaaggtac agttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac
    11881 atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca
    11941 caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa
    12001 agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca
    12061 ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagctgga
    12121 aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg
    12181 gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga
    12241 taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct
    12301 ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa
    12361 gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga
    12421 agtcattctg gttgccgctg acctgacacc gtccgaaacc gcacagctga acctgaagaa
    12481 ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg
    12541 ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga
    12601 cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt
    12661 tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa
    12721 actgaaagat ctgccagcta ttacgctgga cggtcaccaa gtagaagtat gcgctaacat
    12781 tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta
    12841 tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc
    12901 tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga
    12961 catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct
    13021 cggctggcgc gctatccgta tcgcaatgga tcgtaaagag atcctgcgcg atcagctccg
    13081 cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga tgatcatctc
    13141 tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga
    13201 cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc
    13261 cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga
    13321 tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca
    13381 gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga
    13441 aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt
    13501 gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa
    13561 gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca
    13621 accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg
    13681 ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata
    13741 aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag atcattgctc
    13801 cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa
    13861 tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg
    13921 gcaccattgg taaaatcttt gaaaccaacc acgcattctc tatcgaatct gatagcggcg
    13981 ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc
    14041 gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc
    14101 cgcagctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc aacatggacg
    14161 aaatcaaaga actgatcaaa ctgtccggta gcgtgactgt gggtgaaacc ccggttatcc
    14221 gcatcaagaa gtaattcttg ctgcagtgaa aaatggcgcc catcggcgcc atttttttat
    14281 gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc
    14341 acttccagta cgcgcaaccc cgcgcggtgc accgcatcgt ttaatgcctt ccctttcagc
    14401 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc
    14461 cttgaatggg aaatgacatt cacgctgtcg gcagtgacca ccacaaccag catctcctga
    14521 ttttcttcat taccggaggc gctggtaatc accacccatt ttagtgtgtc tgaaagcaga
    14581 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc
    14641 aactcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgct
    14701 tcgggaaggt caggtttgac ataaattccg ctgtcaatat cgccaatcac cggatcgacc
    14761 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    14821 atttgcgatg ccgttcccat atagcccgtg gttacagcac gaagttggcg cagcgcatca
    14881 cgctcctgaa gtgcacgcaa atagccgcta aaccattcat ccggaatcgc accaccgtag
    14941 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa gacattcagg
    15001 ccgttctgtt tgatagcagg cacggcaatg ctgttgccca cgctgccgta aaccacctgc
    15061 gactgcacgg cgacgatatc cgcctgcagc gccctgctct tatcgttaaa caacaacaaa
    15121 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta
    15181 cctgattctg gcagtcaaat cggctattac aaaacaagga taaggtaatt cagtgaagaa
    15241 aatcatttgt ctggtcatta cactactaat gacactcccc gcttacgcga agttaactgc
    15301 ccatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    15361 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttctcatc tgcgtctgaa
    15421 gctgggcaat acccgtaacc gcattgacac tgccgagcag tttattgata aggttgcttc
    15481 gtcgtcatcg attaccggga agccgtatat tgtgaagatc cccggtaaga gcgatgagaa
    15541 tgcacagcct ttttacatgc gttaattgcg cagacggata aaacggtgcc tgcgcaataa
    15601 tcttaaatcc ccgccccctg actaaaatgc tcttccccaa acaccccggt ggaaaggtag
    15661 cgatcgccac gatcgcagat gatcgctacc accaccgcac cagggttagc ttttgccacc
    15721 cgcagtgctc cggcaaccgc gccgccggag ctgacgccac agaatattcc ttcccgcacc
    15781 gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa tatccagcac ctcatctacc
    15841 agagaagcgt tgaaaatccc cggcagatat tccgcaggcc agcggcgaat gccgggaatg
    15901 ctactgccct cttccggctg caggccaaca atggtcaccg gtttggattg ttcgcgcata
    15961 aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc tggagacaaa ataggtaatg
    16021 cgcccgccag tctgctgcca gatttccggc ccggtagtgg tgtaatgggc gtaagggtta
    16081 tcgggattat tgaactgatc gagcagcttt ccttcaccac gatccgccat ctccagcgcc
    16141 agatcgcgcg caccttccat gccctgctct ttggtgacca gaatcagttc cgcaccataa
    16201 gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa tttcatgcga
    16261 tagcctttaa gcgcggcaat cattgccagc gcaatgccgg tgttaccact ggtggcttcg
    16321 attaacacat cacccggttt aatttccccg cgcttttccg cctcgacgat catcgaaagc
    16381 gccgcacgat cttttaccga acctgccggg ttattgcctt ccagttttaa ccacacttca
    16441 ctgccgttat tcggccccat tcgctgcaac ttcaccagag gcgtattgcc tattgtttgt
    16501 tctaatgtac tcacgatctc tatccatacg tggtgttgcc tgatgcgacg cttgcgcgtc
    16561 ttatcaggcc tacaggttac aaaccttgcc attaaaaagc ccggatcgcg ggaagcgcct
    16621 ccgggcgttt aacattcact caacctatca ggcgctttgt gcgagagcaa gttcctcatc
    16681 tcggggttcg atacgctcgt cgccgttata cagccgcgca tgttgcagac caacgaataa
    16741 acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg accgtcagcg gttcgttgta
    16801 ccaccccagc ggctgcacca ctaattgggt gtagtgacct ttcgggctgg cttccagtac
    16861 ctgtaccggc agcggcgaat cgaggctggt acggcggctg atatccactt cccacgggcg
    16921 caggaagaga tccaccggcc cctgatacgc aggtgtgtag cccagcggcc agcgatgtgc
    16981 gccaacatgg aactgcccgc cgcgaatggt tccctgcagg cggttcactt cgcccataaa
    17041 ttcgagcaca aaacgggtcg ccggttcgcg ccatacctga tccggcgcgt cagcctgttc
    17101 aatattgccc tggctcatca caactacacg atcagctact tcggtcgctt cttcctgatc
    17161 gtgggtcaca aaaacgctgg tgaattttag ttcttcatgg agttgacgca gccatcgacg
    17221 cagctcttta cgcacctgcg catccagcgc gccaaacggt tcatcaagca gcagaatttg
    17281 cggttctaca gcaagcgcac gcgccagcgc tacacgctgt ttctggccgc cggaaagctg
    17341 cgccggataa cgatccgcca gatgggcaag ctggaccatt tccagcaatt ttgtcacttt
    17401 cgctttgatg gctgcggcat tcgggcgctc gcgacgcggc agcaccgtca ggccaaaagc
    17461 gatattgtcg aacaccgtca tatggcggaa cagcgcgtaa tgctggaaca cgaaaccgac
    17521 tttacgatca cgtgcgtgca ggcggctcac gtcggtgccg tggaagcgaa tatgcccgct
    17581 ggtttgatgc tccagcccag cgataatgcg cagcagcgtg gttttcccgg aaccggacgg
    17641 ccccagcaac gcgaccatct gacctgaagg aatatccagt gagatatcgt tcagcacctg
    17701 ggtgcgacca aacgacttct taatattggc aatctcaatg ctcatgattt tcctcctgct
    17761 gcgcgcgttt ttcctgattc tccaggcgcc actgcaacat actctttaaa aacagggtga
    17821 taatcgccat cagcgtcaac agcgcggcgg cggtaaagga gccgactgtg ttgtagtcct
    17881 gctccagcaa ttcaatctgt aacggcagcg aaagtgtttc gccacgaatc gagccggaaa
    17941 ccaccgacac cgcgccaaac tcgccaatcg cgcgggcgtt ggtcagcact acgccataaa
    18001 gcagcgccca gcggatgttc ggcaatgtga cacggcggaa catctgccag ccggatgcgc
    18061 caagcaaaat cgccgcttcg tcttcctgac tgccctggct taacatcacc ggcaccagtt
    18121 cgcgcaccac aaacggacac gtcacgaaga tcgtgaccag caccattccg ggccaggaga
    18181 acataatttg cagattatgt tcatccagcc agccgccgag cgggccgttg gagccgtaga
    18241 acagcagata caccagaccg gcaaccaccg gtgacacggc aaaaggaatg tccagtagcg
    18301 tcagcagtaa ctggcgtccg gggaagttaa agcgcgtcac cagccaggcc agcagaatac
    18361 cgaacaccag gtttaccggt acggcaatca gcgcgatcat caccgtcagc cagatggcgt
    18421 gcagcatatc cggatcggcc agattctgta aaaccggcat cagccctttg ctgaacgcct
    18481 gtacgaagat ataaatcatc ggcaccagca ggatgaacgc cgataccagc atcccgatgc
    18541 caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc aattgtgtca
    18601 cttccgccat taatgaccta ccacacgccg accaaagcga ctttgcagag tgttaattga
    18661 gaacagcagc agcagagatg ccgcgaggat caccgaagca atcgcgctcg ccgccgggta
    18721 atcaaactcc tgtaagcgaa cgaaaatcat cagcgacgtc acttccgtct tccaagcgat
    18781 gttcccggcg ataaatatca ccgcgccaaa ctcaccaaga ctacgggtaa acgacaacgc
    18841 cacgcccgcc accagcgccg gagaaagttc cggcagcacc actttgcaga aactctgcca
    18901 gcgcgttgcg ccaagcgttt ctgccgcttc ttcatattcc gggcctaact cttccagcac
    18961 cggctgcacg gtacgtacca caaacggaat gctggtaaag gccattgcca ccgcaatccc
    19021 cagccaggta taggtgactt tgatatcaaa cttcgccagc cattcaccgt aaaaaccgtt
    19081 tacagaaaag agcgaggcca gcgttaaacc ggcgactgcc gttggcagcg caaagggtaa
    19141 atccatcagc gcatcaagca gcgtgcggcc cggaaagcga taacgggtta aaatccacgc
    19201 catcagcagg ccgaaaacgc cgttaaaaat tgatgccaca aacgccgaca gcagcgtcac
    19261 tttgtaggcc gcgaccacct gcgggttggt gatcacttcc cagtactgcg cccagctcat
    19321 ctcagaaagc tgcatcacca gcgcggagag tggcagcagc aatatcaggc acacaaacag
    19381 cagactggtg ccgaggctta aggtaaagcc cggcaggacg cgtcttgatg aaactgcaaa
    19441 cattaattac gccccgccgc taacagcttg tctaactcgc cgccgctggt aaagtgggtt
    19501 ttcatcactt ccggccagga gccaaatttg tcttccacgc ggaacagttc ggtctgcggg
    19561 aatttatctt tcagtttgtc catgacttcc gggttattca cgcggtagta atagtcggtg
    19621 atgatggttt gcgcctgcgg gctgtaaagc cagttcagat aggcttttgc tgctttttcg
    19681 gtaccgttgg cttgcacgtt tttatcgacc cacgccaccg ggaattccgc cagaatgttg
    19741 gttttcggaa tcaccacttc aaagccctgc gcttcgtact gtttgcggat gttgttcact
    19801 tccgattcaa agctaatcag tacatcgccc aggccgcgct cggcaaaggt ggtggtcgcg
    19861 ccacgaccgc cggtatcgaa cacttcaacg tttttcagga actgagtcat aaactgttcg
    19921 gttttggctt tgtcgccacc gtcagcttta tccgctgcgc cccatgccgc cagataggta
    19981 taacgcgcgt tacccgacgt tttcgggttc gggaaaatca gcttcacgtc ggagcgcacc
    20041 aggtcgttcc agtcgtggat attcttcggg ttgcccttac ggaccaggaa gcccatggtg
    20101 gagtagaacg gcgagctgtt attcggcagg cgcgactgcc agtccgccgg gataagcttg
    20161 cctttatcgt gcagaatttg tacgtcggtc acctggttat aagtgacaac gtcggctttt
    20221 aagccctgca aaattgccag cgcctgtttt gatgacccgg catgagattg ttttatcgtc
    20281 agtttgtcgc cgccgttatc tttggcccat tgctgttcaa acggcgggtt cagggcggca
    20341 aacagctcgc gggagacgtc ataagaactg ttcagcagtt ccgttgcctg tacatggccc
    20401 gccagcagca gagaagcgac cagcgcgagt gagttctttt tcagtaagtt aacggccatt
    20461 gcgcaccctt ataaatttaa tgactttcta atagccatca tatttataac ggacgtgaaa
    20521 ggagtaacgg ttttatatac cgtttggtga tttggaagtt gaaaagggaa taagacgaaa
    20581 tcctccccac aaatgcaggg aggaaacaga ggtgaatcag ataccgacgc taaccgtctc
    20641 cggcagtgtg ctgccgccat caatcacatt ctgtgtaccg gttaaatagc tggattcatc
    20701 cgatgcgagg aaggccgcca gttcgccgac ttccagcgga tcggcgaggc gacgcagcgg
    20761 gattgctttt gccatttcag tcagcaccga ctctggatct tccgggttcg actggcgggc
    20821 aatgctttcc gccattggtg tgcgaacgta tccagggcat atggcgttaa cgcgaatacc
    20881 agactgcgca tactccaccg ccagcgattt tgtcaggcca acaatcgccg ctttcgttaa
    20941 ggcgtacgcc gtttcacccg gatcggccac catatcacca gtgactgaag acatcatcac
    21001 aatgcgacca tctttgcggg caatcatctc cggcagcacc gccttcgtga cgttccatac
    21061 gcctttaata ttgatgtcaa tatggaaatc gcgatcgtca tcgctcatat cgaggaaact
    21121 gcccagacga caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt
    21181 cgcgcgtttg atagctgcgg ctaccgacgc cgggtcacgc acatcggcga caaccgccgt
    21241 acagcgatga ccacgaccac acagttcgtc cgccagcttt tcgatctcag gggagatatc
    21301 cagcaagatt aggtttgcgc catgacgcgc aaaagttctg gcaattcctt cgccaattcc
    21361 ctgcaatgcg cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc
    21421 cttgttaata cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt
    21481 actgcggatc agctcactaa ttcaggaaaa tctcaatcca gtcgctgctt tatgcatcat
    21541 ttcaggcgtt gtgttagctc actgctgcgc tgaatcattt tcaatgactc cacatcattg
    21601 agttgcacaa gaccaacaaa cagtaaatcg gtaacagaat tttgcgccgt gcgggtagac
    21661 atcgacgagc tacgccattc ggtttcaccg gaaacggtat cgagcgtgaa atgcgccagc
    21721 cgccgcagcg gagagtcagc cagagaagta atggcaatca ccgttgcccc ctgctttcgt
    21781 gccgcttctg cgcacaagac aatctctttt ttactgccgc tgtaagagat agcgatttgt
    21841 acatcgcctt ttttcaacgc ctgggaaacg gtggcttgta cgtgcgtgtc ggcttcgcag
    21901 gccacgcgat atccaatttt catcagtttg aacgacagat cgcaccccac cagcgccgat
    21961 ccgcccaggc ccgttatctg gataaacggt gctttactga tgacctcaat gattttttgc
    22021 agccgcgcgt agtcgaacaa cgcacaggtt tgctccagcg ccagctcttt ttcgcgattc
    22081 aatttgcggg caatcacttc cagcgaatca tcactggtaa tcgaactgtg caggtgctgc
    22141 gccgtggcat tcgttttctc ccggctggca ctgtattcgc ctattaacgc catacgcaat
    22201 tcagtaaacc cttgtgcgcc gagcttctgg gcaaacttca caatactcga ctggctgatg
    22261 ccgagctgtt tcgccatctt gcgcgaagag acggatttca gctcactcac gttggcccgc
    22321 agaaagtcag caattttttg ctcgttctcg gtaaattcac tttccgcatt gcgtattttc
    22381 gtcaggtaca acatcttcca ctctcctggg ctggcagcaa aaattccgct taagttctta
    22441 tgacgctctt cacactctgc gagtgtagac tcaatgattc ctttaagtct gtcaattcgg
    22501 gatatatgat tccatacatc tgcaattatt tgatcgtaaa tagtaaggtc accaccgatg
    22561 caacttgaaa agatgattac cgaaggctcg aacgccgcct cggctgaaat tgaccgcgta
    22621 tcaacgctgg aaatgtgccg gattatcaac gatgaagata aaaccgtacc gcttgccgtt
    22681 gagcgcgtac tgccggatat cgccgcggca atcgatgtta tccacgccca ggtaagcggc
    22741 ggcggtcgtc tgatttatct cggtgcggga acatccggtc gtctggggat tctggatgcc
    22801 agcgaatgtc cgcccaccta cggcgtgaaa ccgggtctgg tagttggatt aattgctggc
    22861 ggcgaatatg ccattcagca cgcggtggaa ggcgctgaag atagccggga aggcggtgtt
    22921 aatgatttga aaaacatcgg attaaccgcg caggatgtgg tggttggcat tgctgccagc
    22981 ggtcgcacgc cgtatgtgat tgccggactg gaatatgcac gccagctcgg ctgccgtaca
    23041 gtgggaattt cctgtaatcc ggggagcgcc gtttccacca ccgctgagtt tgctattacg
    23101 ccggtggttg gggctgaagt ggtcaccggt tcttcgcgga tgaaagcagg cacggcgcag
    23161 aaactggtgc tcaatatgct ttccaccggg ctgatgatta aatccggcaa agtgttcggc
    23221 aacctgatgg tcgatgtggt cgccaccaac gaaaaactgc atgtgcgcca ggtcaatatc
    23281 gttaaaaacg ccaccggatg taatgccgaa caagcagaag cagcgttaat tgcctgcgag
    23341 cgcaactgta aaacggccat tgtgatggtg ctgaaaaatc tcgatgccgc agaagctaaa
    23401 aaacgcctgg atcaacacgg cggctttatt cgtcaggttt tagacaagga ataacccatg
    23461 gccaaagaga tcagcagtga acttctgaac accattctta cccgtgtcgg cggaccggga
    23521 aatatcgcca gttgtggtaa ctgtatgacg cgcctgcgtc tgggtgtaca tgacagttca
    23581 ctggttgatc ccgatatcaa aacgctggaa ggcgtgaagg gcgtcatttt gaccagtgac
    23641 caggtgcagg tcgtttttgg gcctggtaaa gcgcatcgtg ccgcaaaagc gatgagcgag
    23701 ctgctgggag aagcaccggt acaggatgcc gcagaaatcg ccgcccagaa caaacgtcag
    23761 ttaaaagcca aacaaacctc cggcgtgcaa caatttctcg ccaaattcgc caccatcttc
    23821 acgccgctta tccccggttt tattgccgcc ggtctgctgc tggggatagc gacgttaatt
    23881 gccacggtga tgcacgttcc ggcagatgct cagggcacgc tacccgacgc gctgaatttt
    23941 atgaaggtgt tcagcaaagg tttgttcacc ttcctggtga ttctggtggg ctataacgcc
    24001 gcccaggcat ttggcggcac gggcgtcaat ggcgcaatta ttgccgcact ctttttgctc
    24061 ggttataacc ccgctgcaac caccggttac tacgccggtt ttcacgattt ctttggtctg
    24121 cccatcgatc cgcgcggcaa tattatcggc gtgttgattg ccgcgtgggc ctgcgcccgc
    24181 atcgaaggca tggtgcgccg ctttatgccc gacgatctcg acatgctgct gacctcgtta
    24241 atcaccctac tgatcaccgc tacgctggcc tatctgatca ttatgccgct gggcggctgg
    24301 ttattcgaag gtatgtcgtg gctgtttatg cacctgaaca gtaatccgct cggttgtgcg
    24361 gttttagccg ggctgttcct gatcgccgtg gtatttggcg tgcatcaggg ctttattcct
    24421 gtttacctcg cgttaatgga cagccaagga ttcaacagct tatttcccat cctttcgatg
    24481 gcaggcgcgg gccaggtggg cgcggcgctg gcactctact ggcgggcgca accgcacagt
    24541 gcattacgca gtcaggtacg cggggcgatt attcccggcc tgctgggcgt tggcgaaccg
    24601 ttgatttatg gcgtcaccct gccccgcatg aaaccgttta ttaccgcctg tttaggcggc
    24661 gcggcgggcg gtttgtttat cggattgata gcctggtggg gtctgccgat gggcttaaac
    24721 agcgcctttg ggccgtctgg tctggtggcg ctgccgctga tgacttcagc acaaggcatt
    24781 cttccggcga tggcagtcta cgctggcggg attctggtgg catgggtttg cgggtttatt
    24841 ttcaccacgc tttttggctg ccgtaacgtc aatctggact gatatcatga aacggacaat
    24901 gctctatctt tctctgctgg ctgttagttg tagcgtcagc gccgcaaaat accctgttct
    24961 gacagaaagt tcgccagaga aagcagggtt caacgtcgaa cggcttaacc agatggatcg
    25021 ctggattagc cagcaagtgg acgccggtta tcccggcgta aacctgctga ttataaaaga
    25081 taatcagatt gtgtatcgca aagcctgggg agcggcgaaa aagtacgatg gcagcgtatt
    25141 gatggcgcag cctgtaaaag ctaccaccgg gacgctgtat gacctggcct caaacaccaa
    25201 aatgtatgcc actaacttcg ctctgcaaaa gctaatgagc gaaggcaaac tgcatcctga
    25261 cgatctgatt gcgaaatata tttcggggtt tgccgatagt ccaaatgaca ccatcaaagg
    25321 caaaaacacg ctgcggattt ctgacctgct gcatcacagc ggcggtttcc cggcggaccc
    25381 acaatatccg aataaagcgg tcgcgggcgc gttatattcc caggataaag gccagacgct
    25441 ggagatgatc aagcgcacgc cgctagagta tcagcccggc agcaagcata tttacagtga
    25501 tgtcgattat atgctgcttg gatttatcgt cgagtcggtc accggtcagc cgcttgaccg
    25561 ctatgttgaa gagtcgattt atcgcccgct cggcctgaca catacggtat ttaacccgct
    25621 gctgaaaggc tttaaaccgc aacaaattgc cgccacggaa ttgaacggca atacccgcga
    25681 tggagtgatc cacttcccga atatccgcac ctccactcac tggggtcagg tgcacgatga
    25741 aaaagccttt tattcgatgg gcggcgtttc cgggcacgca ggtttgtttt ccaataccgg
    25801 cgatattgcg gtgttaatgc aaacgatgct gaacggcggg ggctatggcg atgtgcagct
    25861 gttcagtgcg gaaacggtga agatgttcac caccagctct aaggaagatg ccacttttgg
    25921 cctcggctgg cgcgtgaatg gtaatgccac catgacgccg acgtttggca cgctggcaag
    25981 cccgcaaacc tacggtcaca ctggctggac cggaacggtg accgttatcg atccggtgaa
    26041 tcatatggcg attgtgatgt taagcaacaa gccacattcg ccggttgccg atccgcaaaa
    26101 gaatcccaat atgttcgaaa gcggtcagct gccgattgca acgtatggtt gggtagttga
    26161 tcaggtgtat gcggcgttaa agcaaaagta ataaaaaggc cagtcagtaa tgttactgcc
    26221 tggcctttaa tcaatgaatc agaaacgctt acagcgccat caacttgtcc agcgacggtg
    26281 cgaaataata gccgccggtt accggtttgg tgaaacgcaa catcgcatca cgcttaccat
    26341 cggtatcgcc aaacatgctc agcagttgct gctcaatgtt atgcagacgc gcgcagtagg
    26401 cgcagaagta cagaccgtga gtgccactgg cggtgccgta cggcaggctc tggcgaacaa
    26461 tcttcagccc tttgccatct tctttcagat caacgcgggt caggtgagag gtttccggac
    26521 gttcgtcgcc gtcaatctct tcgttggcct ctttggtgcg cccgatcatc atctcctgat
    26581 cgtgaacgct catccggttg agctgcttca ggttgtgctc ccaacgctgc acaaacacat
    26641 agctgccgcc ggcatccacg ccgtctttga taaccgccac ttcgcgacgc gtctcttcac
    26701 ccgccgggtt ttctgtgccg tcaacaaagc cgctcaggtc acgctcttcc acccaacgga
    26761 agccgtggat ctcttctttc acttcaatgc agtcaccaaa ggcttccatc gccgcctggg
    26821 cgacagagaa gtttacgtcg tgacgcagag aaagaatgtg gatcaacaca tcgaactggg
    26881 tggtcggcgc aaggccttta ccgtagcccg gaaaatcttt cagctcttct gccccaacgc
    26941 cgccgctcag agcgcgccag atgttgttac caaaggcaac caccgcacca agatgcgcgt
    27001 ccgggaattt cgcttcaaaa gttgccagtt tgtcggcaaa tgttttactg gccgcacgca
    27061 gggcgtcaac ttcccctttc acgttggctt cgatccaaat cgccgcgcgg caatgttctg
    27121 gcaaaatgcc actctgaacc tgagacattg ttcctcctga aaataataat gccacgcaat
    27181 ccgtggcatt gttgagcgct attttacgtg ttttttcgcg gcggcggttt gttcagacgc
    27241 aaattaacgc cgccagataa ttttactgac tttccagttt ttcagggtat cgtccgaagg
    27301 cattaaccct tcaggaccac tccattcccc gctgaactgg tagctaatat gttgactgcc
    27361 ttcggcttta cattcgacgg cacgattatc atcaccatca gctttttggc aattgccaaa
    27421 tgctttgctg taaaggtcgc taaacggtgt accgatttta acaccggtgt cagcaggaat
    27481 atcgctatcc agcacgtcaa tgcgactgat cgtgccttga tcgccgttaa tcaccatcgc
    27541 cacgttgtcg cccttcatca cttcaaaaaa gcgcaccacg ttgccgttcg cggttttcat
    27601 tccgctgcgc aggcgataat cgccatcaac cgcatcggca atggcttgtt cttgcagtgg
    27661 tgtggacgcc gttaattcac caacgccctg ctcgctcact ttggtggacg acccaaacca
    27721 gttccacggg ttagcggcag accagttaac tgacgacatc gtggaacagc cggtcagcat
    27781 caatggcata gcgcataaca ttaaacgcag cgatttcatg tcacttcctt tggttattca
    27841 ataacgttgc ttggagtgca aattcaccaa aaagtgccgt tattctttta cttcattaaa
    27901 acaagcgcgt aaacgtcgat tggtcagcag ccagattaac gccacaatat ccgccactac
    27961 cagcgccaga ccgataccgc taacggattc accgttcagc cacagccacg gttgccagca
    28021 aagtaaaacc acctgcgcca acagcaacag aaaatagagt acacgccagg tgcgaggaaa
    28081 cgtagcccgc cgaccgctca acaaaaacgc cagcaccgcc ggaatgccag gaatcagccc
    28141 cagccagaaa ttatcgtgat cgggataaaa cagatttagc agcgcagtac cctgctcgcg
    28201 cgacgcaccg gcaatgacaa acagcaccca ggttcgcgcc tgaagcaata gcacaagcca
    28261 gaagagcaag ggtaaacgca ggcgaccgtg cgcatcataa tggacaggat gaaactcagt
    28321 actcttcatc ttcaatcaaa cgcttaccca gactcagcac gtcggcgtgt tcatatccca
    28381 ggcgttcata cattccgagc accatgtcgt tatcttccgg cacgttgatc tgaattttcg
    28441 ggcagccgcg agcaatcagc tttttctcca gccggttaag caacgcattg gcaatcccac
    28501 gcccacgaaa ctctggatgc acgccaagat aataagcaga cccgcgatgc ccgtcataac
    28561 cgcccatcac cgttccgacc acttcaccgt tcacctcagc gaccagaaac aaactgacgt
    28621 catggttcat cttacgctcg atgtccattt ccggatcgtt ccacggacgc agcaagtcgc
    28681 aacgctccca aagggtgatg acctcttcga aatcttcctg gcgaaatacg cgtatctcca
    28741 tggtattcgt tacctttttg cgggttaaaa ggctgattat ggcgtgaacg gtcgaattag
    28801 ccaatatctg acgaaaatcg gttgaaaaag tggcataatg gggagttgtc aactattgaa
    28861 atgaaaagta aaacaattct caacagcaaa cggtcgtaac ggattacgcg atacgatata
    28921 acatctggaa ctttattatt acaactcagg ccgtatgagc acttttaaac cactaaaaac
    28981 actcacttcg cgccgccagg tgctgaaagc cggtttggct gccctgacgt tgtcaggaat
    29041 gtcgcaagcc atcgccaaag acgaaccttt aaaaaccagc aacggacaca gcaagccgaa
    29101 agctaaaaaa tctggcggca aacgtgtcgt tgttctcgat ccaggccacg gcgggattga
    29161 taccggagcg atcgggcgca acggttcgaa agaaaaacat gtggtgctgg cgattgctaa
    29221 aaacgtccgt tccattttgc gtaatcatgg gattgatgcg cgtttaacgc gttctggcga
    29281 tacgtttatc ccactttacg atcgcgttga aatcgcccat aaacatggcg cagatctgtt
    29341 tatgtcaatt catgccgatg gctttaccaa cccgaaagct gccggtgctt cggtatttgc
    29401 cctctctaac cgtggggcaa gtagcgcaat ggcgaaatac ctgtctgaac gcgaaaaccg
    29461 cgccgatgaa gttgccggta aaaaggcgac tgacaaggat catctattgc agcaagtgct
    29521 gtttgatctg gtgcaaaccg atactattaa aaacagcctg acgctcggct cgcatattct
    29581 gaaaaagatt aagccggtgc ataaactgca cagccgcaac accgaacaag ccgcgtttgt
    29641 ggtgttgaaa tcgccgtcgg ttccttcggt gctggtggaa acctcgttta tcaccaaccc
    29701 ggaagaagag cgactgttag gcacggcggc gtttcgtcag aaaatcgcca cagcgattgc
    29761 tgaaggcgtg atcagttatt tccactggtt cgacaaccag aaagcacatt cgaaaaagcg
    29821 ataagttatg aaacccgacg cacaccaggt taaacagttt ctgctcaacc ttcaggatac
    29881 gatttgtcag cagctgaccg ccgtcgatgg cgcagaattt gtcgaagata gctggcagcg
    29941 cgaaggtggc ggcggcgggc gtagtcgggt gttgcgtaat ggtggtgttt tcgaacaggc
    30001 aggcgtcaac ttttctcacg tccacggcga ggcgatgcct gcttccgcca ccgctcatcg
    30061 cccggaactt gccgggcgca gtttcgaggc gatgggcgtt tctctggtag tgcatccgca
    30121 taatccgtat gttcccacca gccacgcgaa tgtgcgcttt tttattgccg aaaaaccggg
    30181 tgcagagcca gtctggtggt ttggcggcgg cttcgattta acccctttct atggttttga
    30241 agaagacgcc attcactggc accgcaccgc ccgtgacctg tgcctgccat ttggtaaaga
    30301 cgtttatccc cgttacaaaa agtggtgcga cgattacttc tacctcaaac atcgcaacga
    30361 acagcgcggt attggcgggc tgttctttga tgatctaaac acgccagatt tcgaccactg
    30421 ttttgccttt atgcaggcgg taggcaaagg ctacaccgac gcttatttac caattgtaga
    30481 gcgacgtaaa gcgatggcct acggcgagcg cgagcgcaat tttcagctct accgtcgcgg
    30541 tcgttatgtc gagttcaatc tggtctggga tcgcggcact ctgtttggcc tgcaaactgg
    30601 cgggcgcacc gagtcgatcc tgatgtcaat gccgccactg gtacgctggg agtatgatta
    30661 tcagccaaaa gatggcagcc cagaagcggc gttaagtgag tttattaagg tcagggattg
    30721 ggtgtaactc cctcaccccc attcccgcat ccgctgatgc aacgtcaacg acggcttctc
    30781 ggcaaacagc tgctggtaat ccgtggcaaa ttgccccaga tgccagaatc cccactgcat
    30841 ggcggcgtct tttaccgttg tgctttgcga ccacgggctt atcagttcgc ggcgtacggc
    30901 gttcaggcga atgcgtttca gccacgcgtt cgggccaatt cctaaaatag cgtgaaacgc
    30961 gttttgtagc gtgcggcggc tgacatgcag ttgattacac aagtccagca ccgtcaccgg
    31021 ttcggacatg ttttccagca catattcacg ggcgcgggaa agcaaccgac ggtaactctg
    31081 atgactgatg ctttccgccg tcaccattgg ctgcgcttct tccagcatcg cccccatcgc
    31141 cattagcaaa ttatccccca gcacttttcg cactgcaggc tgatggagat tttccgggtt
    31201 ctcgcaaaac gtcgccagcg cctgttggac aaagccccac agcgcggctt tatgctgctc
    31261 tttcacttcc agcgccgact ggctacgcaa catatgtaat acccgatccg gattatgcaa
    31321 aaagttagcc tgacgggtga tgacatcttc agaaagcact acgcccagga tcgtgtaatc
    31381 gtccggcgta cttaattcaa actcggttcc accagggcgc gtcgctattt ctgcgcttcc
    31441 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
    31501 cgagttcggc cagaccagac acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
    31561 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
    31621 atcgtaaatc tgctgccagg cggtaatcgt taaagcatgt tcatagacat ccgttgtctg
    31681 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
    31741 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
    31801 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
    31861 gcgtttacgt aacgtcccag cagaaaagag ctgttcgagc gcatttctgg ctttttccag
    31921 cggccagcca aagtgggcag ccacttctcc tgccgtcact ccctgacgta ctgatgttaa
    31981 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
    32041 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
    32101 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
    32161 cagcgcagca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gacctgggtc
    32221 aagcacttcg tgactgagca gacgaacgtt agctgctttg agcatggcat ccgctgcatc
    32281 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca ttggcaacct
    32341 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
    32401 acaggccgct ttacacgctg cctggctacc ggttaaaaat gctgccgagt agttagtttc
    32461 agacggtggc gggacatagg tcaccagctg tacgtcggca gatttcaacg ctgcatcaat
    32521 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
    32581 cccggcggtt gatgagagat aagaaccggt acgcgaaact acatgtgcca ggaatgccgt
    32641 atcttgcgca tcattagccc actggaaagc agcgccattt tcaatatgcg caaccatcgc
    32701 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacctcacc
    32761 ggcagtcggt gacgggccgt gtgccgcgcc cgcatacagc gagcgaccat ataccacttc
    32821 taccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
    32881 agaaatcaga ccgaggctac gaatatgtgg cggcaatttc agttcacgcg caaaatcggc
    32941 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
    33001 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
    33061 cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc gtgccgccct ggtgaatgtt
    33121 agagatacag gtgcgatcgg cctcaacggt ggtcgccata cgcggcgagt aaacggcgta
    33181 gcaggagagg ctttctgact gacccaaccc cggacgttcg cccaccagca ggatcactac
    33241 tttcgcgccg aggatctcac cgatctgatc ttcaatcttc acgcgaccat aacgaacaaa
    33301 gaatggcgtg ccgactttca gcccggcctg tttcaggccc gccatcagcg gcggcaggat
    33361 ctcttcgtag ttcacagtga tggcatcagt tgacaggcca tcagaaataa cgacctgtac
    33421 gtccggatta gcaacacact gtgctttcag cgcttcaaca gcttctgcac acaggcggcg
    33481 gcccatatcc gggcgagtca ggtagagatt tttgtcgcta atttcggagc gtacttccag
    33541 caagccctgc gctttcaccc actcttccgg cacttctttc agtacggtat ctttcgaacg
    33601 agagtgatcg gccaggaaac gcagcagcgc cagcgtacgc ggacgcggac cggcacgacc
    33661 ggtacaaacg cgggccacgg tgctgcgacg cagttctgtt aacacatccg cacgatgtgg
    33721 gttttcaaca ccaatccaca cttttgcttc cgcagaacct aaatccagcg cgcagctttc
    33781 cgaggtcacc ggtgccgcac aggtggtagt ggcgcacttt gcttctgacg gcgcgggggc
    33841 cgtttgtccc attgacgcca tcacgctgcg tacaatttct tcaatctgtt tttgatccat
    33901 gatgtgttat ctccgcgtca tcagaagaac agtgacggat cgcccgcccg tttggtcagg
    33961 cgaccgtttg ccataatgcc catgctttcc agccagcgtt caaactccgg tgacgggcgc
    34021 aggttgagta actgacgcac agtggcggta tcgtggaatg cggtggtctg gtagttgagc
    34081 atgatgtcat cacccagcgg catccccatg atgtagttgc agcctgcggt ggcgagcagg
    34141 atcatcaggt tttcgttgag gttctggtca gcgtcagcgt ggttggtgta acagcagtca
    34201 cagcccatag agatgccgct cagcttgccc ataaagtgat cttctaagcc cgcacggata
    34261 atctggcggt cgttgtagag atactccggc ccaataaagc cgaccacggt gttgacgata
    34321 aacggatcgt aatgacgcgc cagcccatag ttacgtgctt ccatcgtcac ctggtctgcg
    34381 ccgaagttag cgccagcgga tagcgctgaa ccttgtccgg tttcgaagta gaggcagttt
    34441 tccccggcga tacggttgaa ctctgcaccc actgcgcgcg cttcgtcgag catcgccagt
    34501 tccacgccaa actctttcag ccctttttcg ctgccacaga tactctggaa aatcagtccg
    34561 ccaggcgcgc cgcgacgaat cgcttcgatc tgggtggtga cgtgcgccag tacgcagccc
    34621 tgagttggga tgttgaattt gtcgatcacg ccatagatgg tgtccagcac gcggcttaag
    34681 ttttccacgt cgtcagtcac cgggttaacg ccgatcaccg catcgcccac cccgaaggaa
    34741 agcccttcgt agatttgcgc ggcgatactt tgcacgtcgt cacgggtgtc atttggctgc
    34801 aaacgggcgc tgaaggtgcc cggaataccg atggtggtat tggccttttt gattaccggc
    34861 attttcttcg cgccgtagat caggtccgcg ttggagcaaa tcttcgctac cgccgcgacc
    34921 acttccgagg tcagcccttt gcgggtaaag gcaatgtcgt ccacgctggt ttcatcgctc
    34981 agcacatact cacgcagttc gctgatgctc cagtttttaa tctggttgta ggccgtttcg
    35041 ttaacatcgt cctgaatcag ccgcgtcacg cagtcatctt cataggcaat caccggatta
    35101 ttgcggatgt ccgctacggt catttccgac aacacctgct ttgccgccac gcgctcctgt
    35161 gagcttgccg ctgcgacgcc cgccagcaca tcccccgaac gcagttcgtt ggctttagcc
    35221 agcacctctt ttacatcctt aaactgatat acattgccga acaatgtggt ctttagtttc
    35281 ataagtcgtt ccctcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc
    35341 cgccaaaaag aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa
    35401 tgactgccaa cgggagttgt tgtagctgtg ggcgcaacag catgcccaga gctttaccaa
    35461 agtcctgccc ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga
    35521 ccaacgcgtt gatgaccgtc agtaccgcgg cgtaacgcac aggcagcgag gcgggaagcg
    35581 ccagcacgta cgcgtcagtt ttgggatcaa gatccagctg aatcagcgcc tgttgccagg
    35641 cactcaccag atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt
    35701 gtacgccctc cagccagatt gtgctgccag agagcgaaag ggtatgtgcg cccgcgccaa
    35761 tcaccgtggc gcgcacggtt tgcgccggaa actgcacgtt catctcacgc aggcgcggat
    35821 ggtcatgtag cgccgtcgcc agtagcgggc caatatcggc aaaacagaac gggtcagcgg
    35881 gctggtggcg ataacattcg cccacgccgc cagaaagcgt aatgatttcg ggcgtaacac
    35941 ctgcgggcag caaaccggtt tgcattaatg cctgcgcgag cggcgaaagc gttccgtcaa
    36001 tcacttcgac aatcagctct gccatccgtc gggtaacctg caccagctgc gcgccggtca
    36061 gcgaacggac gtcagtgcct gcaccgaaac actcatccac aatcatctgc cccggtttat
    36121 gagcgtaaac cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgaggc
    36181 aggcagtgcc gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga
    36241 tgtcgatatt cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggcaccgt
    36301 gaccggcgat cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg
    36361 actgagagag cgccatcacc gccgggcgag cattgcgggt tttcgcgctt tcaccggtga
    36421 tgatgatggc accagaatca acgctttccg gcgcaatgcc cgcagcctga tattgctcta
    36481 aaatcagcga tttcagttcc gcttctttta aaccaccctg tttatcgaca ggggtaaaga
    36541 acaccgggct ttgccagcta atttcgcgtt tgatgaattc gtagcgcggc acctgcgaca
    36601 ccgccgcacg gttaaccagt tccaggtggg agaaaatcac ctgggtggtg gtggtgccga
    36661 tatcgatacc gacgctcagt agctggcgag tgttcacgat tgtgcctccg cttcggtttt
    36721 agccgcggtc gcgtcgtctt ttggcaccag catcatcgcc acgccaatcg ccgttacgcc
    36781 gccgatcaac ttgccgacaa tcatcgggaa gatcatggca ttcatgttgg cagcggcgaa
    36841 gcctaagtgg tcgcccaggg cgaaagcagc ggaaacggcg aaggcgcagt tgatgacttt
    36901 gccgcgggta tccatctgct tcatcatgcc gaacatcggg atgttgttgg caagcgttgc
    36961 caccatgccg gctgccgcga tgttgttcat attcagcact ttaccgacgc tcatcagcgg
    37021 tttttcaaac cagcgggtca gcagcagcac catcggatac gcccctaaca gaacgcagga
    37081 gatagaaccg ataacttcaa tggcgcgcat cacctcaccg ggtttatcgc caggggccat
    37141 aaagatagga tccagaccgg ggatcagttc ccagccaagc aggaatttca ctaccgcagc
    37201 ggcaagaccg agggtgatca atgcaacgag gaatttggcg aagatctgga agccgttgat
    37261 cattttttct gggatgaatt tcagccccag cgccaccaga atcgcaacaa tgatcaccgg
    37321 gatcatgttc atcaggatca gggcgaaagt gaattccacc ggctggccgt tgatctgaac
    37381 accggagtac atagcaacca gaccaccagc gatacaacca atcggaatgg tcacaatgcc
    37441 cgccagcacg ccgagcgcca gataacgacg gtcagaaggt tcgataatgc cgagcgccac
    37501 cggaatggaa aacacaatcg ttggccccat catcgacccg agaattaacc cagagtatag
    37561 ccacgcggct acgtcgccgc ccgccagctc tttggcgagg aagaagccgc ccatatcgca
    37621 cgccagcagt gttccggcga acatcgatgg gttagcgccg agcatttcgt aaaccggaat
    37681 aattaccggc ccgagtacgt gagccagtac cggtgccagc gcggtcatac cgaccatcgc
    37741 caggcccagt gcgcccattg ccataaagcc ttcttcgaac tgaccgccgg atccttcgat
    37801 acttttaccg aacttaccga ggaaacgagc agaaccgccg aactgtgaca ggatcctgtc
    37861 aacggcagct atcagcataa agaacatcat gatgtacatg atgatttcgt taattcccat
    37921 cgcctttact ccctgttagt tgttatttat tagcggatgc tgcgtgaagc cttatccgca
    37981 ctacatgtgc aatcccacag accggataag acgcggcatt tattgcgccg ctgcgtacag
    38041 gccgacaagc tgttccaggc tggcggtacg cgggttgctg cgcagacaaa tatcttccag
    38101 cgcggcctgc gcccatgcac cgtaatgcgc agatgtcgcg ccaacatcgc ccagtcgttt
    38161 accaatccca acttccgcaa tcagctcact taccgcgtta atagcgtcac gatcgtcgga
    38221 ttttttagtt cgcagtgccc gaccaatctg actaaagcgt tcgcgacaaa ccatccggtt
    38281 aaactccatc accgttggca gcaacatagc gttcgcgaga ccgtgcggaa tatgcagcgc
    38341 cgcgcccggc tgatgcgcca tcgcgtggca caacccaaga cccgcactgg aaaacgccat
    38401 tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcgt gaccgtagcc
    38461 caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca gactgtcggt
    38521 aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg catcaatgcc
    38581 ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaat gcggcgtcga ggatcgccac
    38641 atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg cgtcgataat
    38701 caccgttaca ttggtggttt cagagccggt tccggcggta gttggaatgg caatcagcgg
    38761 caagcgcggt tgcagaacgc tggtttctga catctctgcc agcgtgctat ccgggttcgt
    38821 caccagcaac gccacggctt tcgccgcatc cagcaccgag ccgccgccaa atgcgatcac
    38881 cccgtcacaa cctgactcac gcaactgcgc cacggctgca cacacgtcgg taatgcacgg
    38941 ttcgcccacc ggacatggcc agagcgtcat ggcgatacct ttaacggcca ggctacgcgt
    39001 cagcccggcg gtcatccctg cctgatgcaa aaagctgtct gccatcacga acagatgttt
    39061 cagcccacgc gtttgcgctt gctgtccgca actgctcacc gagcccggac cgcaaagcgt
    39121 caccggtgga acgctaaatg tttttacccg ttgcagattc agggtatcga acgcctgaaa
    39181 gagcgcggtc tgcaattcat tttgcatata gcccctccgc tttctctcgc ccactactgg
    39241 cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt aacgccggga
    39301 attgtttgcg aaacagttct gccacgcccg gttgcataca ggagccgccc gccagccata
    39361 aatcagtaat gccctgccct tcaatatgcc gggcgacaat gtccgccatt ttttcgtaca
    39421 ccggtttcac cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc tccgcctctt
    39481 ccagcgaaat acggcgattt ccggcgaaag tcagagagat gtgatgtccg ccggtggctt
    39541 catccgccga gtacgtcact ttgccctttt tcacgatggc aatgccggta gtgccaccgc
    39601 cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcgactgcg gttggctcat
    39661 ccagcacatg gctcacttcc agcccggcag actccagcac gttgatggaa atacgcgggt
    39721 ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg acgaccgaat tgctgctcga
    39781 gcgtgtcgag atggcgacga acaatggtga ccgcgccgaa gaaatcccag acgatgccgt
    39841 cgcgaaccac atcggcccag tcgaggcata ccgccactgg ctgaccgtcg cggtcgacaa
    39901 ccatcgacac cacatcgcag gtgcccagat ccactcccag ccacagcgga gattccgtcg
    39961 cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg agccattgtt
    40021 cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa tacacagcga
    40081 cgcagacgga caaacgtacg cgcgctggtc accccttcac cggttggcgt ggtgatggtc
    40141 atggtggtcc agccttcccc gcccagcccc agcccggcaa tgcacggtcc gttcttaacg
    40201 aaaatgctgg tatcaatagc attcgccatc tggttcatgt tttcgatgtt gcgcgagtgc
    40261 attgccgccg tgtggtggca accgccttcc agtttcaccg ctagcgcaat ggcatccgcc
    40321 acgttggcga cgcgcacgac gggcaacacc ggcatcatca gttcagtcac ggcaaacgga
    40381 tgttctgcgg tggtttccac aaacagcagg cgcgtttctt gcggaacttt aaggccgatt
    40441 gccgccgcga ttttgcctgc gtcgcgacca acccagtcac ggctgacggt gccttttccg
    40501 cgctcgtcga tatttttcag caacaccggt tgcagctgct gcgcctgttc tgcggtcagt
    40561 ttcaccgcgt gctggccttc catcagacgc atcagttcat cggctacgct atcaacaaca
    40621 atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagacgcgcc tttgacgatg
    40681 gactgagcag cacgggcgag gtcagcggtt tcatccacca ctaccggcgg gttgccagcg
    40741 cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg cttctactac cgcttcgccg
    40801 ccggtcacca ccagcaggcc gatacccgga aacttgaaca agcgttgcgc ggtttcgata
    40861 tccggatttg ccacagtaac cagtaagttt tccggcccgc ctgcggcaac aatcgcctgg
    40921 ttgagcagcg tgatcgcccg ctgggagact tttttcgccg ccggatgcgg ggcaaaaatg
    40981 acgctgttgc ccgcggcaat caggctgatg gcgttattaa ttacggttgc cgccgggtta
    41041 gtggacggcg tcaccgaagc caccacgccc cagggcgcgt tttcaattaa agtcaggccg
    41101 ttgtcgcccg tcagcacttg cggagagaga cactcaacgc ctggcgtgcc gcgtgcctga
    41161 gcgacgtttt tggcaaattt atcttcaacg cgacccatgc cggtttcact gacggcaagt
    41221 tccgctaaat ctctggcgtg tttttcgcct gcttcacgaa tggcagcaat ggctaactgg
    41281 cgcattgcca cgctctttaa cccttgctgg gcaattttgg ctgccgcaac agcgtcatcc
    41341 agggacgcga aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact gctttgcatt
    41401 ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat gttctgcctt
    41461 atttgtggaa aattacctga ccgccagaca ccacctcatc gacgatgcca atcacgcaca
    41521 gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca ctcaccagca
    41581 acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcacat tgcccgtcag
    41641 gattaccatg tggatcaatc atttccacca tcagcaattt gtcatgcgcc agtccgtgat
    41701 ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc gcctccgtgg
    41761 cgtatttcag gtaaaagctc cccctaccct ccgcggaagg taaaatgaaa aatgagagag
    41821 cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc gcctttcagg
    41881 ccgatcggga acacttcttc caggtcgccg tgcggacgcg ggataacgtg tacagatacc
    41941 agctcgccga tacgctgtgc cgctgcggca ccggcatcgg tcgccgcttt acaggctgca
    42001 acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac accaaccagc
    42061 ttgacgcgcg cagctttcac catcgcgtca gaagcctcaa tcagtgcaac caggccccgg
    42121 gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc cagaacggga
    42181 ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc tgacgcggca
    42241 cagctgccac cagcgccagc tcgataattt cctgcacact acaaccacga gagagatcgt
    42301 gcatcggcgc ggcaagtcct tgtatcaatg gcccgacggc acgatatccg ccgagtcgtt
    42361 gtgcgatttt gtagccaata tttccggctt ccagcgacgg aaaaaccatc acattggcct
    42421 tgccctgtag cgggctggca ggcgcttttt gcgccgccac ttccggcacg aaggcggcgt
    42481 caaactgcaa ctcgccatcg actaccagct ttggtgcgcg ctcacggacg atttctgtcg
    42541 cctgctggac attggcaacg caggggtgac gggcgctacc gttactggaa aacgacagca
    42601 tcgccacgcg cggctcttct ccggtgatgg cgcgccaggt ttcggcgctg gcaagcgcga
    42661 tatccgccag ctgcgccgcc gtcggctgtg gtaccacgct gcaatcggca aagcccaacg
    42721 ccggaccgct gtactgtggc agcatcagga aaatggatga gagcgtttta cagcctggct
    42781 gcaagccgat aatgcgtaat ccggcacgca gcacattcgc cgtggaagag aggttgcccg
    42841 cgatacagac atccgcttta ccggcgctga ccattgcggc ggcgaacatc agcggatcgg
    42901 taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc cagcgatgag
    42961 caaattcttc ccgcattgcg aggttgccat gcggatctat cacctgtagc ccgtccatcg
    43021 ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attggcgacc agaatgggcg
    43081 ttgccagacc ttgttgatgt aaatattgcg cagctttcag cacacgttga tctaacgcat
    43141 ccggaaaaac cactctggcg ggcgctcgca acgccagttc acaacaacgt tcaataatca
    43201 ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcggctgct tcgttacgct
    43261 caggatcatc atcacgtaga ccgtgctgga aaggcggttc agcgcctgca agatgtccgg
    43321 gcgtaatact tcaaaactgc gggtgataaa cacctgcgcc gctacggttt cggcttcccg
    43381 cactttggtg cgtagcagat tcagcaacgc cgcatcgcgc ccgtggcttg cttccggcac
    43441 cagatgatcg tgaccaagat aacgcaacgg ctgatgagaa agccggtgca gatcttcgtc
    43501 agaaagcccg acaatcgcct ggcagcccag cggttccgcc agttcaattt gcagccacac
    43561 tgccagcgca atggtgctgt cgagaactgc gcgaaaaccc agacgcggat cgcttttggc
    43621 gaccattttt tccgccgaca ggtgagtcag cgtatccggc ttttttgcca ccggctggcg
    43681 gcacagttcg cagcacgcct gcggatgttc atcgctactg gttagcccat gaacgggctg
    43741 cggctgctgc tgttcatcgt caacaaacag gcggccctgc tcgtcaataa acttgatgcg
    43801 cagatggcgg ctttccagca actcccgggc agagggcgtc aggcgactgt ccgcagggag
    43861 atggatctct gcgccttcgc tgagcgtatg gttcgctctt agccatgctt cggtgatgaa
    43921 atctttcata gggattgcca gttagccggc caggcgacat acaggaattt cacgctggat
    43981 gtcgtaccaa attcgatgct ggagcctttc gggataaaca tcacgtcgcc tgctttagcg
    44041 atcatggttt cgccttcgtg gcgaacatgg agctcccctt ccagcaccat gtcgatttcg
    44101 tcgtagttca gggtccacgg gaagaatgcg ttctcccact gcataaaacc ggcggccatg
    44161 ctgctgccgt cgtctccggt cacaagatca gtcaagccca cgcaatgcgg ttgtgcgcca
    44221 tcaaagcgac caaacttgac gctgctacca tcaatcactt tgatgccgcc tttgccggtc
    44281 accgatttga agctcggctg cattgcaccc tgctccagcg actgtttttc cttcatcact
    44341 ttttccatca gctgcgcgac caggctttcg gtaaactggc cttccggtag ctgggcgata
    44401 atggtttcgc ggatgcgctg gctttcggtt ttatcggctg gcacgctggc gggcacggat
    44461 gccgttaccg ggatcaattc atcacattcg gtgatggtaa aacccagtag atccgccact
    44521 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    44581 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    44641 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    44701 tagctcgaaa atcggctcct cgaagcccgt ctcaagcaat aactttcgcg ttgcggcgac
    44761 gtcagcatcc ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    44821 atccagcaac ccggcaggca accgactttc cgggtcattc gcgccgtgga catagatcag
    44881 catgtccaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    44941 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggcctgtg tttttctggc
    45001 gagggtataa ttcccctgca acgcattaaa taacgttgtt ttccctgccc cgacggtacc
    45061 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    45121 ataatttaat aagcgaccta aaccactgac ggtttgtgat aacgcttctt ctactgcgcc
    45181 caccgagcca taaatcacca atgcaccact gaagcggtcg agaaatccga tatgcacatc
    45241 ggcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
    45301 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct cgccgggatg
    45361 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    45421 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    45481 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcac
//
LOCUS       DAZFKY010000016        44167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFKY010000016 DAZFKY010000000
VERSION     DAZFKY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..146)
                     /locus_tag="ACHKCL_000813"
     CDS             complement(<1..146)
                     /locus_tag="ACHKCL_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5761841.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSD"
     gene            complement(377..1351)
                     /gene="yajO"
                     /locus_tag="ACHKCL_000814"
     CDS             complement(377..1351)
                     /gene="yajO"
                     /locus_tag="ACHKCL_000814"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5761842.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1406..3268)
                     /gene="dxs"
                     /locus_tag="ACHKCL_000815"
     CDS             complement(1406..3268)
                     /gene="dxs"
                     /locus_tag="ACHKCL_000815"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5761843.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3293..4192)
                     /gene="ispA"
                     /locus_tag="ACHKCL_000816"
     CDS             complement(3293..4192)
                     /gene="ispA"
                     /locus_tag="ACHKCL_000816"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5761844.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(4192..4434)
                     /gene="xseB"
                     /locus_tag="ACHKCL_000817"
     CDS             complement(4192..4434)
                     /gene="xseB"
                     /locus_tag="ACHKCL_000817"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5761845.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4640..6088
                     /gene="thiI"
                     /locus_tag="ACHKCL_000818"
     CDS             4640..6088
                     /gene="thiI"
                     /locus_tag="ACHKCL_000818"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5761846.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(6142..6732)
                     /gene="yajL"
                     /locus_tag="ACHKCL_000819"
     CDS             complement(6142..6732)
                     /gene="yajL"
                     /locus_tag="ACHKCL_000819"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5761847.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6695..7606)
                     /gene="panE"
                     /locus_tag="ACHKCL_000820"
     CDS             complement(6695..7606)
                     /gene="panE"
                     /locus_tag="ACHKCL_000820"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5761848.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7774..8265
                     /gene="yajQ"
                     /locus_tag="ACHKCL_000821"
     CDS             7774..8265
                     /gene="yajQ"
                     /locus_tag="ACHKCL_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5761849.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8393..9757)
                     /gene="yajR"
                     /locus_tag="ACHKCL_000822"
     CDS             complement(8393..9757)
                     /gene="yajR"
                     /locus_tag="ACHKCL_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761850.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(9906..10796)
                     /gene="cyoE"
                     /locus_tag="ACHKCL_000823"
     CDS             complement(9906..10796)
                     /gene="cyoE"
                     /locus_tag="ACHKCL_000823"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5761851.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(10808..11137)
                     /gene="cyoD"
                     /locus_tag="ACHKCL_000824"
     CDS             complement(10808..11137)
                     /gene="cyoD"
                     /locus_tag="ACHKCL_000824"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5761852.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11137..11751)
                     /gene="cyoC"
                     /locus_tag="ACHKCL_000825"
     CDS             complement(11137..11751)
                     /gene="cyoC"
                     /locus_tag="ACHKCL_000825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5761853.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11741..13732)
                     /gene="cyoB"
                     /locus_tag="ACHKCL_000826"
     CDS             complement(11741..13732)
                     /gene="cyoB"
                     /locus_tag="ACHKCL_000826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5761854.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13754..14701)
                     /gene="cyoA"
                     /locus_tag="ACHKCL_000827"
     CDS             complement(13754..14701)
                     /gene="cyoA"
                     /locus_tag="ACHKCL_000827"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5761855.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(15161..16636)
                     /gene="ampG"
                     /locus_tag="ACHKCL_000828"
     CDS             complement(15161..16636)
                     /gene="ampG"
                     /locus_tag="ACHKCL_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5761856.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(16680..17258)
                     /gene="yajG"
                     /locus_tag="ACHKCL_000829"
     CDS             complement(16680..17258)
                     /gene="yajG"
                     /locus_tag="ACHKCL_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5761857.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            17563..17880
                     /gene="bolA"
                     /locus_tag="ACHKCL_000830"
     CDS             17563..17880
                     /gene="bolA"
                     /locus_tag="ACHKCL_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5761858.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            18224..19522
                     /gene="tig"
                     /locus_tag="ACHKCL_000831"
     CDS             18224..19522
                     /gene="tig"
                     /locus_tag="ACHKCL_000831"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5761859.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            19768..20391
                     /gene="clpP"
                     /locus_tag="ACHKCL_000832"
     CDS             19768..20391
                     /gene="clpP"
                     /locus_tag="ACHKCL_000832"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5761860.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            20517..21791
                     /gene="clpX"
                     /locus_tag="ACHKCL_000833"
     CDS             20517..21791
                     /gene="clpX"
                     /locus_tag="ACHKCL_000833"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5761861.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            21979..24333
                     /gene="lon"
                     /locus_tag="ACHKCL_000834"
     CDS             21979..24333
                     /gene="lon"
                     /locus_tag="ACHKCL_000834"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5761862.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            24524..24814
                     /gene="hupB"
                     /locus_tag="ACHKCL_000835"
     CDS             24524..24814
                     /gene="hupB"
                     /locus_tag="ACHKCL_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5761863.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25006..26877
                     /gene="ppiD"
                     /locus_tag="ACHKCL_000836"
     CDS             25006..26877
                     /gene="ppiD"
                     /locus_tag="ACHKCL_000836"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5761864.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27028..27399
                     /gene="ybaV"
                     /locus_tag="ACHKCL_000837"
     CDS             27028..27399
                     /gene="ybaV"
                     /locus_tag="ACHKCL_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5761865.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            27505..27903
                     /gene="fadM"
                     /locus_tag="ACHKCL_000838"
     CDS             27505..27903
                     /gene="fadM"
                     /locus_tag="ACHKCL_000838"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5761866.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(27955..28650)
                     /gene="queC"
                     /locus_tag="ACHKCL_000839"
     CDS             complement(27955..28650)
                     /gene="queC"
                     /locus_tag="ACHKCL_000839"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5761867.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(28715..30415)
                     /gene="ybaE"
                     /locus_tag="ACHKCL_000840"
     CDS             complement(28715..30415)
                     /gene="ybaE"
                     /locus_tag="ACHKCL_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5761868.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            30515..31333
                     /gene="cof"
                     /locus_tag="ACHKCL_000841"
     CDS             30515..31333
                     /gene="cof"
                     /locus_tag="ACHKCL_000841"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5761869.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            31485..31943
                     /gene="decR"
                     /locus_tag="ACHKCL_000842"
     CDS             31485..31943
                     /gene="decR"
                     /locus_tag="ACHKCL_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5761870.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            31973..33745
                     /gene="mdlA"
                     /locus_tag="ACHKCL_000843"
     CDS             31973..33745
                     /gene="mdlA"
                     /locus_tag="ACHKCL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5761871.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            33738..35519
                     /gene="mdlB"
                     /locus_tag="ACHKCL_000844"
     CDS             33738..35519
                     /gene="mdlB"
                     /locus_tag="ACHKCL_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5761872.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            35700..36038
                     /gene="glnK"
                     /locus_tag="ACHKCL_000845"
     CDS             35700..36038
                     /gene="glnK"
                     /locus_tag="ACHKCL_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5761873.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            36068..37354
                     /gene="amtB"
                     /locus_tag="ACHKCL_000846"
     CDS             36068..37354
                     /gene="amtB"
                     /locus_tag="ACHKCL_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5761874.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(37403..38263)
                     /gene="tesB"
                     /locus_tag="ACHKCL_000847"
     CDS             complement(37403..38263)
                     /gene="tesB"
                     /locus_tag="ACHKCL_000847"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5761875.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            38481..39053
                     /gene="ybaY"
                     /locus_tag="ACHKCL_000848"
     CDS             38481..39053
                     /gene="ybaY"
                     /locus_tag="ACHKCL_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5761876.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(39086..39397)
                     /locus_tag="ACHKCL_000849"
     CDS             complement(39086..39397)
                     /locus_tag="ACHKCL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5761877.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            39560..39656
                     /gene="ffs"
                     /locus_tag="ACHKCL_000850"
     ncRNA           39560..39656
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKCL_000850"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            39776..40129
                     /gene="ybaA"
                     /locus_tag="ACHKCL_000851"
     CDS             39776..40129
                     /gene="ybaA"
                     /locus_tag="ACHKCL_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5761878.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(40171..41721)
                     /gene="pdeB"
                     /locus_tag="ACHKCL_000852"
     CDS             complement(40171..41721)
                     /gene="pdeB"
                     /locus_tag="ACHKCL_000852"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5761879.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(41885..42355)
                     /gene="ylaC"
                     /locus_tag="ACHKCL_000853"
     CDS             complement(41885..42355)
                     /gene="ylaC"
                     /locus_tag="ACHKCL_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5761880.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(42471..43022)
                     /gene="maa"
                     /locus_tag="ACHKCL_000854"
     CDS             complement(42471..43022)
                     /gene="maa"
                     /locus_tag="ACHKCL_000854"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5761881.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(43194..43412)
                     /gene="hha"
                     /locus_tag="ACHKCL_000855"
     CDS             complement(43194..43412)
                     /gene="hha"
                     /locus_tag="ACHKCL_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5761882.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(43438..43812)
                     /gene="tomB"
                     /locus_tag="ACHKCL_000856"
     CDS             complement(43438..43812)
                     /gene="tomB"
                     /locus_tag="ACHKCL_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5761883.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11100 a        10928 c        11688 g        10451 t
ORIGIN      
        1 ttatcactgt ccagaaaaaa atcacgaatt ttgttccaca gcctttcaat aagattttga
       61 tttaaatctt gcttcattga gtttcttaaa tctgcaaccg gtacatcagt aaacaggatc
      121 ggttgtttgg tgatggtttt catcatgatt gtaatcctct tacgttgact aaataagaca
      181 gttttagggg atattacctg gagaaactat caaaaatcgc tcaaagggat gaagacgatg
      241 aatagctgca aactcattca cgaagaattt ctcttgcaga ggtggattat ggttacagaa
      301 ggtgatggga ggtacgttgt ccccatcatc gcagacgatg gggaattcag cagataaaat
      361 gggtgaaaag cgaaatttac ttaaatccta cgaccggatg cggtttatac ggtgtttcca
      421 gttcagcaat ctgttccggc ttcaaagtga tatccaccgc gttcagcagc tcatcaagct
      481 gttcttcccg cgaagttccg ataatcggtg cggcaatgcc cggtttactc aacaaccagg
      541 ccagcgcaac ttgtgctcgt gttgcaccga gttcttcact gacgcccgtt aaccgctctg
      601 cgatctgcgc gtcattttca tcgctttctt tatagagatt tttccccacc tcatcagaca
      661 ccagtcgtgc ggtagtttct ccccacggac gcgtcagtcg cccccgcgcc agcggactcc
      721 acggaatgac cgccacgcct tcctgataac acagcggcag catctcgcgc tcttcttcgc
      781 gataaatcag attgtagtga tcctgcatac tgacaaactg cgcccagccg tgctgttttt
      841 gtagttccag tgcctgagca aactgcgaag cgtgcatgga tgacgcgccg atataacgcg
      901 ctttcccggc ttttaccacg tcgttgaggg cttccagcgt ctcttcgatc ggcgtgttgt
      961 aatcccagcg atgaatttgc aggatatcga cataatccat gccgagacgt cgcaggctgt
     1021 cgtcgataga gcgcaaaatt tgtgcacggg ataatccttc cggtaaatca ccaacgcgat
     1081 ggaacacttt ggtggcaacg accacgtctt cacgacggga gaaatcccgc agtgcgcgac
     1141 cgacgatctc ttcgctgctg ccatcggaat agctattggc ggtatcaaag aaatttatgc
     1201 cgccttccag tgcgcgttta atgatgggac ggctgctctc ttccggcagt gtccatgcgt
     1261 gattaccacg atctggctcg ccaaaggtca tacagccgag gcaaagtcgg gaaacgcgaa
     1321 ggtcggtttt tcctaagggg ttgtattgca tgctgccact cctgccatga ataatttctt
     1381 aagcatagca ggagtggagt agggattatg ccagccaggc cttgattttg gcttccatac
     1441 cggcggcatc gaggccgagt tcggcgcgca tttcttcctg agttccttgc ggaataaaga
     1501 agtccggcag gccaatgttc agcacgggta ctggtttacg atgggccatc agcacttcgt
     1561 tcactccgct gcctgcaccg cccataatgg cgttttcttc tacggtaacc agtgcttcat
     1621 gactggcggc catttccaga attaacgttt catcaagcgg tttcacaaaa cgcatatcga
     1681 ccagcgtggc gttcagcgat tcggcgactt tcgccgcttc tggcatcagc gtaccaaagt
     1741 taaggatcgc cagtttctcg ccacgacgct tcacaatgcc tttgccaatt ggtagttttt
     1801 ccagcggcgt cagttccacg ccgaccgcgt tgccacgcgg gtagcgcacc gctgacgggc
     1861 catcgttata gtgatagccg gtatagagca tctggcgaca ttcgttttca tcgctcgggg
     1921 tcataatgac catttccggt atgcagcgca ggtaagagag atcaaaagcg ccctggtggg
     1981 tttgaccgtc agcaccaaca atgcccgcgc ggtcgatggc gaacaggacc gggagttttt
     2041 gaattgccac gtcatgcagc acctgatcat aggcgcgttg caggaaggtg gagtaaattg
     2101 ccacgatcgg tttgtagcca ccaatcgcca gaccggcggc aaaggtcacc gcgtgttgct
     2161 cggcaatcgc tacatcaaaa tagcgatccg ggaatttacg tgaaaactcg accatgccgg
     2221 aaccttcacg catcgccgga gtaatcgcca tcagtttgtt atctttcgct gccgtttcgc
     2281 acaaccagtc gccaaagatt tttgaatagc ttggtaaacc accgctactt ttcggcaaac
     2341 aaccgctgga gggatcaaat ttaggcacgg cgtggaaggt gattgggtct ttttctgccg
     2401 gttcataacc acgacctttt ttggtcatga tatgcaggaa ctgcgggcct ttcaggtcgc
     2461 gcatgttctt cagcgtggtg ataagcccca gcacatcgtg accgtcaacc gggccgatgt
     2521 agttaaagcc cagctcttca aacaacgtgc caggcaccac catgccttta atatgttctt
     2581 cggtacgttt gagcagctct ttaattggcg gaacgccaga gaaaactttt ttcccgcctt
     2641 cgcgcagtga agagtaaagc ttaccggaaa gcagctgcgc cagatggttg ttaagcgcgc
     2701 caacattttc ggaaatcgac atttcattgt cgttgaggac caccagcata tcaggacgaa
     2761 tatcgcccgc gtgattcatc gcttcaaacg ccatgcctgc ggtaatcgcg ccgtcgccaa
     2821 tcacacagac ggtgcggcga tttttgcctt ctttctcggc agcaaccgca ataccaattc
     2881 cggcactgat ggaggttgat gaatgcccga cgcttaatac gtcatattcg ctttcgccgc
     2941 gccacgggaa cgggtgtaga ccgcctttct gacggatggt gccgattttg tcgcggcgtc
     3001 cggtcaaaat tttatgcgga taagcctgat gccccacatc ccaaatcaat tggtcaaacg
     3061 gggtgttata gacatagtgc agcgccacgg tcagttcgac cgtgcccagc ccggaggcga
     3121 agtgcccgct ggaacggctc acgctgtcga gtaaatagcg gcgcagttcg tcgcagagtt
     3181 tcggtaaact ctcttttggc aacagtcgta gctcctgggt ggagtcgacc agtgccaggg
     3241 tcgggtattt ggcaatatca aaactcatca ggggcctatt aacacttatt gtttatttat
     3301 tacgctggat gatgtagtcc gctagcgctt ccagtgccga agtatcgatc gactgttcag
     3361 ccagttgttt cagcgactgg cgggcatcgt cgatcagatc ctgggctttc ttccgggctt
     3421 gctcaagacc caggagtgca gggtaggtac ttttgccaag ctgctggtcg gcaccctgac
     3481 gttttcccaa cgttgcagta tctcccacca catccaggat gtcatcctga acctggaagg
     3541 caaggccgat gctctctgcg tacttgtcga gtactggtag agcacgacgt cctttatctc
     3601 cggcgcttaa tgcaccaagg cgaacggcgg cgcgaattaa tgcgcctgtt ttatgacgat
     3661 gaatacgctc aagcgcgtcc agaggtacgt gtttgccttc cgcgtctaaa tccaacgcct
     3721 gaccaccaca cattccggca ataccgctgg cgctcgccag ttcagaaatc atcgaaattc
     3781 tgtcgcgatc cgacacttct ggcatatcag catcgcttaa aatcgagaac gccagcgttt
     3841 gtaaagcgtc gccagcgaga atcgcgttcg cttcgccaaa cttcacatgg caggtcggca
     3901 agccgcgacg cagatcgtca tcatccatcg ccggtaaatc atcatgaatt aatgagtaag
     3961 cgtggataca ctctacggcg gcagcgggtg cgtccagcgt gtttgtgcta acgccgaaca
     4021 tatgaccggt ggcataaacc aggaaaggtc gcaggcgctt accacctaat aatgcgccat
     4081 actgcatggt ttcgaccacg ggagtgttct gaaagggcag tggggcgata aaacggctca
     4141 gcgcctggtt ggcctgctta acgcaggctt cgagttgctg cggaaagtcc attactcatt
     4201 gtccggtgta aaaggggtta gagaggcgtc ttcgttgtca gacagcagaa tttgtacgcg
     4261 ctgttcggct tgttgtaatt tggcctgccc ctgacgtgcc agctgcacgc cgcgttcgaa
     4321 ctcgttcagc gcctcttcca gcggcaggtc gccactttcc agacgggtta caatctgttc
     4381 cagctcgctc agcgcctttt caaagctggc gggcgcctca tttttcttcg gcataatgaa
     4441 tgtctgactc tcaatatttt tcgccccgtc atggtaacgg actcagggca aatagcaaat
     4501 aacgcgcaat ggtaaggtga tgtgcgcagc aaagcgatgt tagtggtata cttccgcgcc
     4561 tggatgcagc cgcaggtgtg ggctgctgta tttttcccta tacaagtcgc ttaaggcttg
     4621 ccaacgaacc attgccgcca tgaagtttat cattaaattg ttcccggaaa tcaccatcaa
     4681 aagccaatct gtgcgcttgc gctttataaa aatccttacc gggaacattc gtaacgtttt
     4741 aaagcactat gatgagacgc tcgctgtcgt ccgccactgg gataacatcg aagttcgcgc
     4801 gaaagatgaa aaccagcgtc tgactattcg cgacgccctg acccgtattc ctggtatcca
     4861 ccatattctc gaagtcgaag acgtgccgtt taccgacatg cacgatattt tcgagaaagc
     4921 gttggttcag tatcgcgatc agctggatgg caaaaccttc tgcgtacgcg tgaagcgccg
     4981 tggcaaacat gattttagct cgattgatgt ggaacgttac gtcggcggcg gtttaaatca
     5041 gcatattgaa tccgcgcgcg tgaagctgac caatccggat gtgactgtcc atctggaagt
     5101 ggaagacgat cgtctcctgc tgattaaagg ccgctacgaa ggtattggcg gtttcccgat
     5161 cggcacccag gaagatgtgc tgtcgctcat ttccggtggt ttcgactccg gtgtttccag
     5221 ttatatgttg atgcgtcgcg gctgccgcgt gcattactgc ttctttaacc tcggcggcgc
     5281 agcgcatgaa attggcgtgc gtcaggtggc acattatctg tggaaccgct ttggcagctc
     5341 ccaccgcgtg cgttttgtcg ctattaattt cgagccggta gtcggggaaa ttctcgagaa
     5401 aatcgacgac ggtcagatgg gcgttatcct caaacgtatg atggtgcgtg ccgcgtctaa
     5461 agtggctgaa cgttacggcg tacaggcgct ggtcaccggc gaagcgctcg gccaggtgtc
     5521 cagtcagacg ctgaccaacc tgcgcctgat tgataacgtc tctgatacgc tgatcctgcg
     5581 tccgctgatc tcttacgaca aagagcacat catcaacctg gctcgccaga ttggcaccga
     5641 agactttgcc cgtactatgc cggaatactg cggtgtgatc tccaaaagcc cgacggtgaa
     5701 agcggttaag tcgaagattg aagcggaaga agagaaattc gacttcagca ttctcgataa
     5761 agtggttgaa gaagcgaata acgttgatat ccgcgaaatc gcccagcaga ccgggcagga
     5821 agtggtggaa gtggaaaccg tcaatgactt cggcccgaac gacgtgattc tcgatatccg
     5881 ttctgtcgat gaacaggaag ataagccact gaaagtcgaa gggattgatg tggtttcact
     5941 gccgttctac aagttgagca ccaaatttgg cgatctcgac cagaacagaa cctggctact
     6001 gtggtgtgag cgcggggtaa tgagccgcct gcaggcgctc tatctacgcg aacagggctt
     6061 taagaatgtg aaggtgtatc gtccgtaatt tgtggttttt acgtcgcatc tggtcagatg
     6121 cgacgtttgc cgcatccgac actactcgta ataattataa atccctgccg ccatcaccag
     6181 ttgtgatgcc acttcatggg ctttctcgcg cccaaccagc aggtcgataa ttttcagacc
     6241 aaagtcgata gctgtacccg gcccctggct ggtcagcaat tttacccgtg catcccagac
     6301 gacgcgcttg tcctgccatt gttcagcggg aattttgtct ttcagcgtcg ggaagccggt
     6361 catattgcca atcggaaaga tatcgtgcgg caccagcacg gtggctggcg cggcgcaaat
     6421 agccgcgacg atacgcccgg aacggtggaa ctgtttaacg gtttcaacca gcagggtact
     6481 atcgcgaaaa cactccgcgc ctttaatgcc accaggcagc acgatcacgt catattcgcc
     6541 atcagccact tcgaccagcg gcgcatccgc cagcagcttc acgccgcgcg agcaggtaat
     6601 cgccaggttg ccatcgctgg ctacgctggc agtggtgact ttgataccgc cgcgaatcaa
     6661 gaggtcgatc gtggtgacgg cttcagtctc ttcactacca ggggcgaggc aaaccagtgc
     6721 cgatgcgctc atattcactc tcctttcttt ttaccatttc aaacaggcgg gtgttttccg
     6781 gtacggcaat cccatgcgcg cgggcgcggc gtaagagaaa accattgata tagtcgattt
     6841 cggtgtggcg cagcgtgcgg atatcctgca acatcgacga gatattttcc gctgtggcat
     6901 caatcacctg catcacgtaa tcacgtaaat cttccgctga agtatgatgc ccttcgcgtt
     6961 cgatcactgc cgcgacttct tcgcatattt gcataatttc ttgcggatga tgacgtaatt
     7021 caccgttcgg gcaattccag atggcagtca gtggattaat cacacagttg actgccagct
     7081 tgcgccacag ctcggcgcga atattgttat gccaggcaac gtcaggcaac acggtttgca
     7141 atatatccgc cagataactg taatcgccgt cctgttgccg tgctggacca atatgcgtga
     7201 taccgtttgc cacatgaata atgacattgc cgtcgcggca ggcggcatgg gtggtggtgc
     7261 ccatcagtaa tggctgctga atgttttgca attcttcgat ggtgcccatg ccgttgtgaa
     7321 ttaacagtat tggcgtagtt acaggcagtg tggacgcgag gcttttgacg gcatcggaaa
     7381 cctgccatgc tttcagcgtc accaggagca gatcgctggt ggcaagaaaa tcgggatcgt
     7441 tggcggtcag cgattcgtta aatatcgaac catcagtctc aaccagattc acactacaat
     7501 aaggttgcgg tacgcgcagc cagccctgaa cttcgtgacc ctgtttacaa agtgctgtaa
     7561 gccataattg ccctaaggca ccgcatccca atacggtaat tttcattgtt cctcctcacc
     7621 cgcaaccact ccgggtgttc aataaggcta tcccttaatt gtgcatgctg ttgcgactat
     7681 gcacaattaa gggatacgtc ctggtgcagg actgttggtt atttaacttt gcaggtatta
     7741 tgcttcgcat caaaaatgaa gggagaggaa aagatgccat ctttcgatat tgtctctgaa
     7801 gttgatcttc aggaagcacg taacgcggtc gataacgcga gccgcgaagt ggagtcccgt
     7861 tttgacttcc gtaacgttga agcctcattt gagctgaacg acgccagcaa aaccatcaaa
     7921 gtgttgagcg agtccgactt ccaggtcaat cagttgctgg atattctgcg tgccaagctg
     7981 ctgaagcgcg gcattgaagg cagttcactg gatgtaccgg aaaatatcgt tcatagcggt
     8041 aaaacctggt ttgtggaagc gaaactgaaa cagggcattg agagcgccac tcagaaaaaa
     8101 atcgtcaaga tgatcaaaga cagcaaactg aaagtgcagg cgcaaattca gggcgatgag
     8161 atccgtgtga cgggcaaatc tcgtgatgat ttacaggctg tcatggcgat ggtgcgcggt
     8221 ggcgatctcg gtcagccgtt ccagttcaaa aacttccgcg attaatcgcg acaacctgcg
     8281 ctttgttcat gtcagatgcg gcatgaacgc ctggttcggc ctacaaagtt ttgtaaatca
     8341 ggaacttagt aggcctgata agcgtagcgc agcaggcaat ttcgcctcgc ttttatgcct
     8401 ggcgaattgc ctgttcaaca tcaaagcgat tcgtcacttt gctgtcgatt ttcacataag
     8461 ctgaatgttc ttcttctgca atcaacactt ctttgatgcc ttcagtttcc agcaaacgca
     8521 cttttaacgc ctcgtttgcg gcaatgttcg ccgggatttc aatgcgcaaa ctgctgacat
     8581 acggcggttc tttcatggta ctggcgactg ccagccacac tgcggccagc attgcgccag
     8641 cgagaaatac cccctgaccg tcaaacatgc cgtcaatcca gccgcccagc gaaccgccaa
     8701 tcgccacgcc aagaaactgg ctggtggagt aaacacccat cgccgtacct ttgtaacctg
     8761 ctggcgactc tttactgata agtgagggca ggagggcttc catcaaatta aacgccacaa
     8821 agaaaagctg cacgccgacc accagttgcc agaactgcgt ttgcgcgttc ctcaacacaa
     8881 tttccgcaac cacgatcaac ccgacgcaga agacaaagac ttgcttcatt ttgcgcttaa
     8941 cttcagcgta gataatgaaa ggcacgaccg agccaaaggc gattagcatt gtcgccagat
     9001 agaccttcca gtgttcagcc gccgggaacc ccgcatcagc cagttgtccg ggcagggcaa
     9061 caaacgtcga catcagcaaa atatgcagac acataatgcc aaagttgagt ttcagcagcc
     9121 gcggttccgc cagcacctta ctgaaactgc ctttcaccat tccggactca cgattaagta
     9181 cgtgagtgct actgttgggc acaacccaaa tggtcaacgc aatgccggtc gttgccagaa
     9241 tagcgatcat ccagaacagc gcgtgcagcc caagtttgtg agtgatgatc gggccaagca
     9301 ccatcgcaat ggcaaaggta atgccaaagc tcacgccgat aaacgccatt gctttggtgc
     9361 ggttttgttc gcgcgtgaga tcggaaagca gcgccataac ggcagcggca atcgcaccgg
     9421 agccttgtag cgcccgaccc agaataattc cccagatgga gtcagagagc gcagcgataa
     9481 cgctaccggc ggcaaacact gccagcccac cgacaattaa tggtttgcga ccaatgcggt
     9541 cggaaagcag gccaaacgga atctgaaaaa cggcctgagt cagaccataa ataccaatgg
     9601 caataccgat taatgcttcg ctggcacctt gcagagccat gccgtacgtg gtcagaaccg
     9661 gcagaaccat gaacatgccc agcatgcgca acgagaatac ggtccctaaa ccccaggtcg
     9721 cgcgccgctc acctggcgtc attttataat cgttcattac cacctctgtt ttaaattcgc
     9781 gactagtgta aagcggcaaa cgaatggggt aaacatgcgg attttttagt aaatatttcg
     9841 agagggatat gtttctaatg ctaagaaaaa aggtgccgta gcaccttttt aatagagagg
     9901 ttgtgttacc acacagcagc cagcagcgta tgcgagtccg gcaccataaa atcaacggac
     9961 atcatcaccg agagggcagt gatggcgatg atagagaagc cgaacagctt gcgcgcccag
    10021 attctgtcat cagcaacttt ataaccgcgc agagccatac ctaaccacca gacgctaacc
    10081 gccgcggcga ccaccagata tttatatcca gcgtaaccgc caagagagag catcagcgtg
    10141 gcaacggcaa aggcgatgat atacagcgtg atgtgattct tcgccaccga aatgcctttt
    10201 accactggca ataccggaat gtttgccgcc tggtaatcct taaagcggaa aatggcgatg
    10261 gcataggagt gaggcatctg ccacaggctg aagatagcca gcaggatcgc tgcgccgcta
    10321 tcgaactcgc cggttactgc acagtagcca atcaccggcg gcgcagcgcc ggagagcgaa
    10381 ccaatcaatg tgccgtagac ggagtggcgt ttcatgtaca ggctataaac gccgacataa
    10441 accacaaagc ccatcacccc cagccagcag gccagcggat tcgcgccaaa ccacagcagc
    10501 ataaagccag caatacccag caaggtggcg tacaccagcg agacagcagg agagatcagg
    10561 cctttaacca gcacccgatt cttcgtcctt tccatctttc tgtcgatatc cctgtcgatg
    10621 tagttgttaa acacacaacc cgacgccaca accagtgaca ccccaaccag cgtgtagata
    10681 aacaggggat aatcaatgct gccctttgag gccagcagga atcccccaat caccgagatc
    10741 aggttgccaa agatgatgcc tggtttcgtt acttgcaggt attgcttaaa catcataacc
    10801 gccgctctta gtgcatcatc atgttgtagt tgaggttcca cataatccag atggagccta
    10861 caactaggat agcgatgatt agcacggtga agacaaacgc cgtcatgttc cagccttcat
    10921 ctgatttggt attcatgtgc aggaagcaca ccagatgcac cagaacctgt accactgcca
    10981 ttgccaggat tgttcccaga attacggccg gagaggcagc ccctgtcatc accatccaga
    11041 acggaatcac cgtcagaatg atcgacagga taaagcctgt catgtaggtt tttacgctgc
    11101 catgggacgc gccgctgtga tcggtagaat gactcattac atcgccccca tcagataaac
    11161 aacagtgaac acacagatcc aaaccacatc caggaagtgc cagaacaggc tcaggcacat
    11221 gatgcgggta cggttagtgc tggtcaggcc gcgacgagcg atttgtacca tcagcaccgc
    11281 catccagata agaccggaag tgacgtgcag accgtgcgtg ccgaccagcg caaagaacgc
    11341 tgacaggaag ccgctgcgat ccggacccat gccgttaaca atcaggtgat ggaattcgta
    11401 gatttccatc ccgataaatc cggcgccaaa caaccaggtc aacgccagcc aggagataac
    11461 ctggcttttg ttgtttttgt acatggcgat agccgccatg ccgtaggtga tggagctgaa
    11521 caacagcaag aaagtttcaa ccagaacgaa cggcagttcg aaaatgtcct tacctgtcgg
    11581 gccgcctgcg gtgccgttca ccagaacggc ataggtagca aacaagatag agaacagaat
    11641 gcagtcgctc atcaggtaga tccaaaatcc gaagattttg gttccgcctg catcgtggtg
    11701 cccgtgttcg tgcgcgtgga cagtcgcgtg cgtcaaagta tcagttgcca tttttcagcc
    11761 ctgccttagt aatctcatcg aaatgctggt tttccagttt ttcgatttct gccaccggca
    11821 cgtagtaatc cacgtcctcg tcgaagcttt tcacgatcca ggtgatgatc atgcctgcga
    11881 agccaacaat cgccagccac cagatatgcc agatcatggc gaaaccgaag atggtggaga
    11941 aagccgcaat gacgatacct gcaccgctgt ttttcggcat atgaatttct tcatagtggt
    12001 caggcttttt atacgcttcg cctttctctt tcatttccca gaatgcatca cgctcgtgaa
    12061 cgtgcggcac aacggcaaag ttatagaacg gaggcgggga agaggttgcc cactccagcg
    12121 tacggccacc ccacgggtcg ccagtcaggt cacggttctg gtcgcggtca cgaatagaaa
    12181 cgtacatctg aataacgagg cagagaatac ctagcgcaat cagtactgca ccgctggctg
    12241 caatcatcag catggtgtgg aactgcgggt caatctgctg gctcaaacga cgggtcatgc
    12301 ccatgaagcc cagcgcgtac agtggcataa aggcaacgaa gaagccgatg atccagaacc
    12361 aaaacgcgcg tttaccccag gtttcgttca gtttgaaacc gaacgcttta ggccaccagt
    12421 aggtcatccc tgcgaagcag ccgaagacca cgccgccgat gatcacgtta tggaagtgcg
    12481 caatcaggaa caggctgtta tgtagaacga agtccgcgcc cggtactgcc agcagcacgc
    12541 cagtcatacc gcccaccgag aaggtgacga taaaaccgat agtccacagc atcgcagaat
    12601 ggaacacgat gcggccctga tacatggtga acagccagtt gaagatcttc accccggtcg
    12661 ggatggcgat aatcattgtg gtgataccaa agaaggcgtt tacgttcgcg cccgcaccca
    12721 tcgtaaagaa gtggtgcagc caaacgatga acgacagcac ggtgatacag acggttgccc
    12781 ataccagcga ggtataacca aacagacgtt tacgcgagaa ggttgccgca atttcggaga
    12841 acacaccgaa aacaggcagg atcaggatgt aaacttccgg gtggccccag gcccaaatca
    12901 ggttgatgta catcatcatg ttgccaccca tatcgttggt aaagaaatgg gtgcccagat
    12961 agcgatccag ggtcaacaac gcgacggtaa ccgtcagaat tgggaaggaa gcaataatca
    13021 gtacgttcgc gcacagtgat gcccaggtaa atactggcat cttgaacatg gtcatgcccg
    13081 gtgcgcgcat cttcagaatg gtaacgaaga agttgatacc ggtaagcgtc gtacctatac
    13141 cggatagctg gagactccat atccagtaat cgacaccgac tcccggactg tactctattc
    13201 ccgatagcgg tggataggcc agccagccgg tctgcgcaaa ttcgcccacg ccgagagaaa
    13261 cgttaaccag aatcacacca acaacggtaa accagaagct taagttgttg aggaacggga
    13321 acgcaacgtc acgcgcgccg atctgcagcg gaaccaccaa gttcatcaga ccgataacga
    13381 aaggcatcgc cacgaagaag atcataatca cgccgtgcgc ggtgaagatc tgatcgtagt
    13441 ggtgaggtgg caggaagccc gcttcgcccg ccgaggcaag agcctgctgg ctacgcatca
    13501 taatggcgtc agcaaaacca cgcagcaaca tcacaatcgc cacgatgata tacatgatac
    13561 cgaggcgttt atggtcgacg gaggtcagcc actctttcca caggtaggtc cacttaccga
    13621 agtaagtgat caggccaacg agcgccagac ctcccaaaat aatgccagcg atcgtaacca
    13681 tgacgatagg ttcatggaac gggactgcat caagtgataa ttttccgaac atctttattc
    13741 ttcctcaacc cctttaatgg gcggattccg cgtggctcat gtccatgcct tccatacctt
    13801 cgtgtgcgct gtgctcacct tctggctggg tcatgtccat gctcttaccg tgagccataa
    13861 acttgttaat tacatcggca aacaagtttg gtttcacgtt ggagaaatat tccacctggt
    13921 tgtattcgct aggcgcggcc agtttttcga acgctgccat gtcagacatg gagttcggcg
    13981 actgtttcgc ttttgcgacc cactggtcga atgcggcgcg atccggtgtt gcaatagctt
    14041 tgaacttcat gcctgagaag cccgggccgc tgtagctggc ggagataccg tcataagtgc
    14101 cgggttcgtt ggcgatcaga tgcaggcgag tctgcatacc ggccatggca taaatctggc
    14161 tacccagacg cggaatgaag aaggagttca tcacggagtt ggaggtcact ttgaagtaca
    14221 ccggagtgtt cgccgggaaa gcgatttcat tcacggtagc aaagccctgt tccgggtaga
    14281 tgaagaacca tttccagtcc atggaaacca cttcgatggt aatgggcttc tcgtcgtgtg
    14341 ccagcggctt gctaggctca agagcgtgag tggttttcca ggtcagtacc gcaaggaaga
    14401 tgatgattaa gataggtacc gtccagacca cagcttccac tttattggag tgtgaccagt
    14461 tcgggctgta cttagcatct ttattgctcg cacggtactt ccaggcgaaa ccaacagcca
    14521 tcaagattgc gggaataacg acaatcaaca tcaggccaaa tgccgtcagt atcagtgaac
    14581 gttgctccag accaatctgt cctttgggat ctaacagcgc agaattacag ccactgagca
    14641 atacagtgcc tgcaaataat gacaaccatc ccaaactttt attgtatttc ctgagtctca
    14701 tttaacgacc tcaattccac gggatctggt ggcgtttaaa atgtgtgggc attttacggg
    14761 aaggttacat tactgtaaac atgattaaat tcctgttact tggatttggc tgtcaggtca
    14821 catatgttgc aaaacatatc aggttgattt taagaagctg ttgcaaaagg gcgttataac
    14881 aaaaggggag tgcagaagaa gtctggacgg gcaattggta taatcaatgc gaaataaaac
    14941 aattatttaa caaataatta tcattggagt gacaacttgg tggtgagaaa ataaagactc
    15001 attagctgaa tcgtgtaaga aaaatagaat ttataaatgg cgccaataat ttccaaacgc
    15061 taagccgcac aaaagaacaa atattaataa gcgatgctga tgtttattta gccgtaataa
    15121 ttacggcgag tgattactac agctaaataa tatttacaga ttacgtcaga tgcgtttttc
    15181 gtagcgccag ataatccagc aaaccaccaa gcacgacgcc agaaagggcg actaaaaccc
    15241 cgacttccag cagagcaggc aggaaagaga agtgcgtcaa atccagcgcg tccatcgtca
    15301 atagcagtaa ccacacggcc aacaggctga cgcccgccgc cagtgtccac atggcaaagg
    15361 cataacctgc cggatatgcg gtacgggaga taaagttgtc atttactcgt gtatattcaa
    15421 gcgtctggcg gcaaaccagc agcaaaataa gccctggtac ggcagcggcg acggagaata
    15481 gatagaatgt cgaccagccg tgtgcttcaa caaaccaacc cgccacgggg ccgacataaa
    15541 ctcgccctac agcagaaagc gctgagagca gggcaaattg agtagcggaa aatgacttat
    15601 tacatagcgt cattaacagc gcgacaaagg ctgatgtgcc catcccgcca cagaggtttt
    15661 cgaaaaagac ggctgcgccc atgctgtaga gatgcttatc agtaatcgac agcagccagt
    15721 aaccagcgtt agacgcacct tgtaaaatgc cgaaaatcag cagtgcccgg aacagtgaca
    15781 ggcgctgcat caaaatccca ccgtacaatg cgccaacaat ggtcgctagt aagccaagcg
    15841 ttttgtttac cacgcctact tcacccgcat caaacccgac gccgcgaatc aaaaacgtgg
    15901 ttgtcaggct catggcgaat gcgtcgccca gcttatacag cacgataaga agcaaaataa
    15961 gccaggcatt attgcgacca aagaaatctc gcagaggtgc aacaaccgct tgttccagcg
    16021 ttttgggcac aggaatggtg tcggttggtt ctggtgcaag caacgtcgcg ataatacagg
    16081 ggatcaacag cgccgccatc aaccagtaca tgccctgcca gcccagccat ttatctgcca
    16141 gccacagggc taggccgccg gaaaccagca tccctaaacg gtaacccaga acgctgattg
    16201 ccgcgcccgc accacgttct tctgccggga gcacatcggt tttccacgca tcgaagacaa
    16261 tatcctggga ggcagagcaa aaagcgatca ccaccgccag cgccgccatc cagcggagtt
    16321 gggtgcctgg ttcgagaaaa cccatcgccg caatggcgac taataacagg atttgcgtgg
    16381 cgagcagcca accgcgccgc cgcccaaaaa atgggggcgt gtagcggtcc atcagcggtg
    16441 accagaggaa tttaaaaacg taagcctggc ctaccagaga gaagaaacca atggttttga
    16501 gatcgatatt ctcgaccgtc atccaggcct gtaaggtgcc ggatgtcagg gcgagcggta
    16561 gcccggaagc aaaacccagg atcagcaata tggctgaacg cggctgttga aaaatacgta
    16621 aatattgact ggacatgggc gtctacaggc ccggcttgca ccgggccaga gggcagaaat
    16681 taacgcgcgt tctgcttgat gaattcgtga atgctggtgt cctgagacat atcagcgatg
    16741 gtatcggtca gcacactgtt aaccgcatcg gcgatatttt tgttggaggc ctggaacgca
    16801 ccttcaacgt tgtagctggc acgatagttt ttggtcattt tgttgccatt ctgcgcggta
    16861 gcgatgatgg cgatatccgc tttggtagcg atgttgtagc gcacgttgcc ctgggacacg
    16921 tcagcataca gctggctaac gatgatttgc agattaaccg ggccattcgg accaaccatg
    16981 taaccacgcg cggtcatctg tttttccagc acttcttgca gcaggaaacg cagatcgcgg
    17041 gaggcggtca gggtaacgat ttgattatcg cgggtgactt ttgccagcgc ctgatcggta
    17101 cgctgatcgg caccattaat gcttacggtg acgcccatca ggcttggatc ctgctgtggc
    17161 agtgtaatcg tcggggaaac ttcaatagtt gttggcggtc ttgcgcatcc tgcaagcata
    17221 aacagagcaa ctaacgggaa gaggattttt ttgaacatgt tcgggctctc agagactctt
    17281 aagcgtgttt ggtaaaaatt cccgccatca taacattgcc aacggcgagg ggaagtaggt
    17341 aaggcatgta aattcatcat gttgacgaaa taatcgcccc tggtaaaaga aacgctgatg
    17401 cgaggcctgt gtttcaatct ttaaatcagt aaacttcata cgcttgacgg aaaaaccagg
    17461 acgaaaccta aatatttgtt gttaagctgc aatggaaacg gtaaaagcgg ctagtattta
    17521 aagggatgga tgacatctca gcgttgtcgg aggagatatt tcatgatgat acgtgagcgg
    17581 atagaagaaa aattaagggc ggcgttccaa cccgtattcc tcgaagtagt ggatgaaagc
    17641 tatcgtcaca atgtcccagc cggctctgaa agccatttta aagttgtgct ggtcagcgat
    17701 cgttttacgg gtgaacgttt tctgaatcgt catcgaatga tttacagtac tttagcggag
    17761 gaactctcta ctaccgttca tgcgctggct ctgcatactt acactattaa ggagtgggaa
    17821 gggttgcagg acaccgtctt tgcctctcct ccctgtcgtg gagcaggaag catcgcgtaa
    17881 aaacgcattt gcaactgtcg gcgcttttcc agtatgttgc taaagatttt atgaaaaacg
    17941 gcctgcgggc cgttttgttt tgtctggatt ttgcgctttt tgcccagcat tcagacgaaa
    18001 attgcccggg aattgtgaaa aaatacgcga cagcgcgcaa taaccgttct cgactcataa
    18061 aagtgatgcc gctataatgc cgcgtcctat ttgaatgctt tcgggatgat tctggtaaca
    18121 gggaatgtga ttgattataa gaacatcccg gttccgcgaa gccaacaacc tgtgcttgcg
    18181 gggtaagagt tgaccgagca ctgtgatttt ttgaggtaac aagatgcaag tttcagttga
    18241 aaccactcaa ggccttggcc gccgtgtaac gattactatc gctgctgaca gcatcgagac
    18301 cgctgttaaa agcgagctgg tcaacgttgc gaaaaaagta cgtattgacg gcttccgcaa
    18361 aggcaaagtg ccaatgaata tcgttgctca gcgttatggc gcgtctgtac gccaggacgt
    18421 tctgggtgac ctgatgagcc gtaacttcat tgacgccatc attaaagaaa aaatcaatcc
    18481 ggctggcgca ccgacttatg ttccgggcga atacaagctg ggtgaagact tcacctactc
    18541 tgtagagttt gaagtttatc cggaagttga actgcagggt ctggaagcga tcgaagttga
    18601 aaaaccgatc gttgaagtga ccgacgctga cgttgacggc atgctggata ctctgcgtaa
    18661 acagcaggcg acctggaaag aaaaagacgg cgctgttgaa gcagaagacc gcgtaaccat
    18721 cgacttcacc ggttctgtag acggcgaaga gttcgaaggc ggtaaagcgt ctgatttcgt
    18781 actggcgatg ggccagggtc gtatgatccc gggctttgaa gacggtatca aaggccacaa
    18841 agctggcgaa gagttcacca tcgacgtgac cttcccggaa gaataccacg cagaaaacct
    18901 gaaaggtaaa gcagcgaaat tcgctatcaa cctgaagaaa gttgaagagc gtgaactgcc
    18961 ggaactgact gcagaattca tcaaacgttt cggcgttgaa gatggttccg tagaaggtct
    19021 gcgcgctgaa gtgcgtaaaa acatggagcg cgagctgaag agcgccatcc gtaaccgcgt
    19081 taagtctcag gcgatcgaag gtctggtaaa agctaacgac atcgatgtac cggctgcgct
    19141 tattgacagc gaaatcgacg ttctgcgtcg ccaggctgca cagcgtttcg gtggcaacga
    19201 aaaacaagct ctggaactgc cgcgcgaact gttcgaagaa caggctaaac gccgcgtagt
    19261 tgttggcctg ctgctgggcg aagttatccg caccaacgag ctgaaagctg acgaagagcg
    19321 cgtgaaaggc ctgatcgaag agatggcttc agcgtacgaa gatccgaaag aagttatcga
    19381 gttctacagc aaaaacaaag aactgatgga caacatgcgc aatgttgctc tggaagaaca
    19441 ggctgttgaa gctgtactgg cgaaagcgaa agtgactgaa aaagaaacca ctttcaacga
    19501 gctgatgaac cagcaggcgt aatttacgca gcataacgcg ctaaattcgc acaaaggccc
    19561 gtcaccgcca ggtggtgggc ttttttttgt catgaatttt gcatggaacc gtgcgaaaag
    19621 cctctttcgg tgttagcgta acaacaaaag attgttatgc ttgaaatatg gtgatgccgt
    19681 acccataaca cagggactag ctgataatcc gtccataagg ttacaatcgg tacagcaggt
    19741 tttttcaatt ttatccagga gacggaaatg tcatacagcg gcgaacgaga taactttgca
    19801 ccccatatgg cgctggtgcc gatggtcatt gaacagacct cacgtggtga gcgctctttt
    19861 gatatctatt ctcgtctact taaggaacgc gtcatttttc tgactggcca ggttgaagac
    19921 catatggcta acctgattgt ggctcagatg ctgttcctgg aagcagaaaa cccagaaaaa
    19981 gatatctatc tgtacattaa ctccccaggc ggggtgatca ctgccgggat gtctatctat
    20041 gacaccatgc agtttatcaa gcctgatgtc agcaccatct gtatgggcca ggcggcctcg
    20101 atgggcgctt tcttgctgac cgcaggggca aaaggtaaac gtttttgcct gccgaattcg
    20161 cgcgtgatga ttcaccaacc gttgggcggc taccagggcc aggcgaccga tatcgaaatt
    20221 catgcccgtg aaattctgaa agttaaaggg cgtatgaatg aacttatggc gcttcatacg
    20281 ggtcaatcat tagaacagat tgaacgtgat accgagcgcg atcgcttcct ttccgcccct
    20341 gaagcggtgg aatacggtct ggtcgattcg attctgaccc atcgtaattg atgccagagg
    20401 cgcaactgtg ccgctatact tatccagggc ggcacaacgc tgtaagcggc ttgcgcctga
    20461 gaatggcatt tgcgtcgtcg tgtgcggcac aaagaacaaa gaagaggttt tgacccatga
    20521 cagataaacg caaagatggc tcaggcaaat tgctgtattg ctctttttgc ggcaaaagcc
    20581 agcatgaagt gcgcaagctg attgccggtc catccgtgta tatctgcgac gaatgtgttg
    20641 atttatgtaa cgacatcatt cgcgaagaga ttaaagaagt tgcaccgcat cgtgaacgca
    20701 gtgcgctacc gacgccgcat gaaattcgta accacctgga cgattacgtt atcggccagg
    20761 aacaggcgaa aaaagtgctg gcggtcgcgg tatacaacca ctacaaacgt ctgcgcaacg
    20821 gcgataccag caatggcgtc gagttgggca aaagtaacat tctgctgatc ggtccgaccg
    20881 gttccggtaa aacgctgctg gctgaaacgc tggcgcgcct gctggacgtc ccgttcacca
    20941 tggccgacgc aaccacgctg accgaagccg gttatgtggg cgaagacgtt gaaaacatca
    21001 ttcagaagct gttgcaaaag tgcgattacg acgtacagaa agcgcagcgc gggattgtct
    21061 acatcgatga aatcgacaag atttctcgta agtcagacaa cccgtctatt acccgtgacg
    21121 tttccggtga aggcgtacag caggcactgt tgaaactgat cgaaggtacg gtagctgctg
    21181 ttccaccgca aggtggacgt aaacatccgc agcaggaatt cttgcaggtt gatacctcta
    21241 agatcctgtt tatttgtggc ggtgcgtttg ccggtctgga taaagtgatt tcccatcgtg
    21301 tagaaaccgg ctccggcatt ggttttggcg cgacggtaaa agcgaagtcc gacaaagcaa
    21361 gcgaaggcga gctgctggcg caggttgaac cggaagatct gatcaagttt ggtcttatcc
    21421 ctgagtttat tggtcgtctg ccggttgtcg caacgttgaa tgaactgagc gaagaagctc
    21481 tgattcagat cctcaaagag ccgaaaaacg ccctgaccaa gcagtatcag gcgctgttta
    21541 atctggaagg cgtggatctg gaattccgtg acgaggcgct ggatgctatc gctaagaaag
    21601 cgatggcgcg taaaaccggt gcccgtggcc tgcgttccat cgtagaagcc gcactgctcg
    21661 ataccatgta cgatctgccg tccatggaag atgtcgaaaa agtggttatc gacgagtcgg
    21721 taattgatgg tcaaagcaaa ccgttgctga tttatggcaa gccggaagcg caacaggcat
    21781 ctggtgaata attaaccatt cccatacaat tagttaacca aaaagggggg attttatctc
    21841 ccctttaatt tttcctctat tctcggcgtt gaatgtgggg gaaacatccc catatactga
    21901 cgtacatgtt aatagatggc gtgaagcaca gtcgtgtcat ctgattacct ggcggaaatt
    21961 aaactaagag agagctctat gaatcctgag cgttctgaac gcattgaaat ccccgtattg
    22021 ccgctgcgcg atgtggtggt ttatccgcac atggtcatcc ccttatttgt cgggcgggaa
    22081 aaatctatcc gttgtctgga agcggcgatg gaccatgata aaaaaattat gctggtcgcg
    22141 cagaaagaag cttcaacgga tgagccgggt gtaaacgatc ttttcaccgt cgggaccgtg
    22201 gcctctatat tgcagatgct gaaactgcct gacggcaccg tcaaagtgct ggtcgagggg
    22261 ttacagcgcg cgcgtatttc tgcgctctct gacaatggcg aacacttttc tgcgaaggcg
    22321 gagtatctgg agtcgccgac cattgatgag cgagaacagg aagtgctggt gcgtactgca
    22381 atcagccagt tcgaaggcta catcaagctg aacaaaaaaa tcccaccaga agtgctgacg
    22441 tcgttgaata gcatcgacga tccggcgcgt ctggcggata ccattgctgc acatatgccg
    22501 ctgaaactgg ctgacaaaca gtccgttctg gagatgtccg acgttaacga acgtctggaa
    22561 tatctgatgg caatgatgga atcggaaatc gatctgctgc aggttgagaa acgcattcgc
    22621 aaccgcgtta aaaagcagat ggagaaatcc cagcgtgagt actatctgaa cgagcaaatg
    22681 aaagctattc agaaagaact cggtgagatg gacgacgcgc cggacgaaaa cgaagccctg
    22741 aagcgcaaaa tcgacgcggc gaagatgccg aaagaggcaa aagagaaagc ggaagcagag
    22801 ttgcagaagc tgaaaatgat gtctccgatg tcggcagaag cgaccgtagt gcgtggttat
    22861 atcgactgga tggtacaggt accgtggaat gcgcgcagca aggtcaaaaa agacctgcgt
    22921 caggcgcagg aaatccttaa taccgaccat tatggtctgg agcgcgtgaa agatcgcatc
    22981 cttgagtatc ttgcggttca aagccgtgtc aacaaaatca agggaccgat cctttgcctg
    23041 gtagggccgc cgggggtagg taaaacctcc ctgggtcagt ccattgccaa agccaccggg
    23101 cgtaaatatg tccgtatggc gctgggcggc gtgcgtgatg aagcggaaat ccgtggtcac
    23161 cgccgtactt acatcggttc tatgccgggt aaattgatcc agaaaatggc gaaagtgggc
    23221 gttaaaaacc cgctgttcct gctcgatgag atcgacaaaa tgtcttctga catgcgtggc
    23281 gatccggctt ccgcactgct tgaagtgctg gatccagagc agaacgtggc cttcagcgat
    23341 cactacctgg aagtggatta cgatctcagc gacgtgatgt ttgtcgcgac gtcgaactcc
    23401 atgaacattc cggcaccact gctggatcgt atggaagtga ttcgcctctc cggttatacc
    23461 gaagatgaaa aactgaacat cgccaaacgt cacctgctgc cgaagcagat tgaacgtaat
    23521 gcactgaaaa aaggtgagct gaccgtcgac gatagcgcca ttatcggcat tattcgttac
    23581 tacacccgtg aggcgggcgt gcgtggtctg gagcgtgaaa tctccaagct gtgccgtaaa
    23641 gcggttaagc agttactgct cgataagtca ttaaaacata tcgaaattaa cggcgacaac
    23701 ctgcatgact accttggtgt tcagcgtttc gactatggtc gcgctgataa cgaaaaccgt
    23761 gtcggtcagg taaccggtct ggcgtggacg gaagtgggcg gtgacttgct gaccattgaa
    23821 accgcatgtg ttccgggtaa aggcaaactg acctataccg gttcgctcgg cgaagtgatg
    23881 caggagtcta ttcaggctgc gttaacggtg gttcgcgcgc gtgcggaaaa actggggatc
    23941 aaccctgatt tttacgaaaa acgtgacatc cacgtccacg taccggaagg tgcgacgccg
    24001 aaagatggtc cgagtgccgg tattgctatg tgcaccgcgc tggtttcttg cctgaccggt
    24061 aacccggttc gtgccgatgt ggcaatgacc ggtgagatca ctctgcgtgg tcaggtactg
    24121 ccgatcggtg gtttgaaaga aaaactactg gcagcgcatc gcggcgggat taaaacagtg
    24181 ttaattccgt tcgaaaataa acgcgatctg gaagagattc ctgacaacgt aattgccgat
    24241 ctggacattc atcctgtgaa gcgcattgag gaagttctga ctctggcgct gcaaaatgaa
    24301 ccgtctggca tgcaggttgt gactgcaaaa tagtgacctc gcgcaaaatg cactaataaa
    24361 aacagggctg gcaggctaat tcgggcttgc cagccttttt ttgtctcgct aagttagatg
    24421 gcggatcggg cttgccctta ttaaggggtg ttgtaagggg atggctggcc tgatataact
    24481 gctgcgcgtt cgtaccttga aggattcaag tgcgatataa attataaaga ggaagagaag
    24541 agtgaataaa tctcaattga tcgacaagat tgctgcaggg gctgatatct ctaaagctgc
    24601 ggctggccgt gcgttagatg ctattattgc ttccgtaact gaatctctga aagaagggga
    24661 tgatgtagca ctggtaggtt ttggtacttt tgccgttaaa gagcgtgctg cccgtactgg
    24721 ccgcaacccg cagaccggta aagagatcac catcgctgcc gctaaagtac cgagcttccg
    24781 tgcaggtaaa gcactgaaag acgcggtaaa ctaagcgttg tccccagtgg ggatgtgacg
    24841 aagttcaagg gcgcatctac tgatgtgcct tttttatttg tattcggtga ctttctgcgt
    24901 cttgtgggct gacaattgcc cccgtttctt gtcacaatag gcctttgcgc gcatcgatac
    24961 gttgcgtgag gtacacagtc atctacagcg gagtgttgtt acaccatgat ggacagctta
    25021 cgcacggctg caaacagtct cgtgctcaag attattttcg gtatcattat cgtgtcgttc
    25081 atattgaccg gcgtgagtgg ttacctgatt ggcggaggca ataactacgc cgcaaaagtg
    25141 aatgaccagg aaatcagccg tgggcaattc gagaatgcct tcaacagcga gcgtaatcgc
    25201 atgcagcaac agctgggcga tcaatactcc gagctggcag cgaacgaagg ctatatgaaa
    25261 accctgcgtc aacaggtgct gaatcgtctg atcgacgagg cgctgctgga tcagtacgca
    25321 cgtgagctga aactgggtat cagcgatgag caggttaaac aggcgatttt cgcgacccca
    25381 gccttccagg ttgatggcaa atttgataac agccgctata acggtatcct caaccagatg
    25441 gggatgaccg ccgatcagta cgcccaggcg ctgcgtaacc agctcactac ccaacagctg
    25501 attaacggcg ttgctggtac cgattttatg ctgaaaggtg aaaccgacga gctggcggca
    25561 ctggtcgctc aacaacgcgt ggtgcgtgaa gcgactatcg atgttaacgc gctggcggcg
    25621 aagcagcctg tgaccgaaca ggaaattgcc agctactacg aacaaaacaa aaacaatttc
    25681 atgacgccgg aacaattccg cgtgagttac atcaagctgg atgccgcaac gatgcagcaa
    25741 ccggttagcg atgcggatct ccagagctac tacgatcagc atcaggatca attcacccag
    25801 ccgcagcgta cccgttacag catcatccag accaaaactg aagatgaagc gaaagcggta
    25861 cttgatgagc tgaataaagg cggtgatttt gctgcattag ccaaagaaaa atctgccgat
    25921 attatctctg ctcgtaacgg cggcgatatg ggttggttag aagatgccac tatcccggat
    25981 gaactgaaaa atgctggtct gaaagaaaaa ggccaactgt ctggtgtcat caaatcttcg
    26041 gtcggtttcc tgattgtacg tctggacgac attcagccag cgaaagtgaa atcgttagac
    26101 gaagtacgtg acgatgtcgc ggcgaaagtg aaacacgaaa aagccctcga tgcgtactac
    26161 gcgctgcagc agaaagtgag cgatgcggca agcaacgaca ccgagtctct ggccggtgca
    26221 gagcaagctg ccggcgttaa agccactcag acgggttggt tcagcaaaga gaacctgccg
    26281 gaagagttga acttcaagcc ggttgctgac gctatcttta acggcggtct ggtaggtgaa
    26341 aacggcgcgc cgggcatcaa ctctgacatc atcaccgtag acggcgaccg cgcattcgtg
    26401 ctgcgcatca gcgagcacaa accggaagcg gtgaaaccgt tggcagatgt tcaggaacaa
    26461 gttaaggcac tggttcagca caacaaagct gaacaacagg cgaaagtgga tgctgagaaa
    26521 ctgctggttg atttgaaagc cggcaaaggt gcggaagcta tgcaggctgc cggtctgaaa
    26581 tttggcgagc cgaaaacctt aagccgttcc ggtcgtgacc cgattagcca ggcggcgttt
    26641 gcactgccac tgccagcgaa agacaaaccg agctacggta tggcgaccga tatgcaaggt
    26701 aatgtggttc tgctggcgct ggatgaagtg aaacaaggtt caatgccgga agatcagaaa
    26761 aaagcgatgg tgcagggtat cacccagaac aacgcacaaa tcgtctttga agctctgatg
    26821 agtaacctgc gtaaagaggc gaaaatcaaa attggcgatg cgttggaaca gcaataatcc
    26881 tgaagccgcc tcgcaaaaaa atgctttgca gctgtaacaa gaaaaggtcg ctttcgcggc
    26941 cttttccatt tctgaacatt gccatttgtt tactgttttc actgccgtta aggtgattcc
    27001 actgttaaca aacaaggaga aaacagtatg aaacacggaa ttaaagcact gctcattacc
    27061 ctgtccctgg cctgtgccgg aatgtctcat agcgcgctgg cggcagcttc tgtggcgaaa
    27121 ccgacggcgg tagaaaccaa agcggaagct cctgcagcac aaaataaagc agcagtaccg
    27181 gcgaaagcca gtgacgaaga aggctcccgg gtcagcatta ataatgccag cgcggaagag
    27241 ctagcccgcg cgatgaatgg cgttggcctg aagaaagcgc aagcgattgt cagttatcgc
    27301 gaagagtacg gtccgtttaa aacggtggag gatctaaagc aggtgccggg gatgggcaat
    27361 tcgctggtgg aacgtaatct ggcggtatta accctgtaat taatttgcat agtggcaatt
    27421 ttttgccaga ctgaagaggt cataccagtt atgacctctg tacttataac agtttttata
    27481 aaaacaacgt aaggttattg cgctatgcaa acacaaatca aagttcgtgg atatcatctc
    27541 gacgtttacc agcacgtaaa caacgcccgc taccttgaat ttctcgaaga agcccgctgg
    27601 gatgggttgg aaaatagcga cagttttcac tggatgacgg cccataacat cgctttcgtg
    27661 gtggtgaata tcaatattaa ctatcgtcgt ccggcggtat taagcgactt gttaaccatt
    27721 acgagccagt tacagcaatt aaacggtaaa agcggcatct taagccaggt cattacactg
    27781 gagccggaag ggcaggtggt agcggatgcg cttattacgt ttgtttgtat tgatcttaaa
    27841 acgcagaaag cattagctct ggaaggggaa ttgcgcgaaa agctggagca gatggttaag
    27901 taaacgtttt gtggtgccgg atgctcaagc cgcatccggc gacacccgga ataattactt
    27961 caacccggtt ttctgcttca tcgctgccat caccgtcggt ttatcggcca gataatgatt
    28021 caaaccgttg gcgcgtaaat tacatgccgc acaatgaccg caaccgtcgc ctttaatgcc
    28081 gttatagcag gtcaacgttt cgttacggac taaatccagt ttgccgtaat aatctgccag
    28141 cgcccaggtt tccgctttat caatccacat cagcggcgtt tcaaaacgaa tatctttcgc
    28201 catgcccaaa ctgacggcat ggtttagtac tttcacaaac tcatcgcggc aatccgggta
    28261 gccggagaaa tccgtttcgc agacgccggt aattacggct tctgctttta cctgatacgc
    28321 atatattgcc gccagcgtca ggaacaaaat attacgccct gggacaaacg tattcgggat
    28381 gccatcggct tcaggttcat aatcaggcac cggaatgctg tcacgcgtca ggctgctgac
    28441 cgccagctcg ttgagcagcg ttacatcaag aaccttatgc gcgcgtgccc ccagtttcag
    28501 cgccagttcg cgtgccacgt cgatttctgc gcgatggcgc tgaccgtaat cgaacgtcac
    28561 gcaatggact tcatcatatt gttgtaatgc ctgcaccaga caggtggtgg aatcctggcc
    28621 tccactgaac acaacgacag cacgtttcat agataatcct gcttgaacaa taaaagcgtt
    28681 atggtaacgc ctgcgattaa cccggaccag ctattcattg cgacggcgcg ggaagccagg
    28741 cttcggtaaa ttcaaaccag ccgcgcggtg tcagtcgcac accgttcacg ccgggagggg
    28801 cactaatgcg atagtgatag ttgaacagcg gcgtcagcgt cgcatctgtc attaactggc
    28861 taaaaaccgc tttcagggca ttaaatcggt tttcctcatc aggcattact tgcaccgcgt
    28921 ccagtgtcga ttgtagatgt gcgtatgctg gagcgtcgaa aacatgtggc cacagcggat
    28981 cgcagcgcag ccattgctcc agagtatatt ccggtgcttc gccaattaat ctgtcgccca
    29041 tcatgaggtc tgcgtgtgcc agtagggtcg tgtcgtccca gttttttgcg ttatgaaaaa
    29101 taattgtgag ttcacagcct tccgctgcca gtgtcgcctg taggcgttct gccatggtat
    29161 gaagttctat cgggaggtga taaaccagcg tcaaggtttt cggtagtttg acttcatccg
    29221 gtacttgcca atgcggaata gtccagcctg gcagtaatgc atgactggcg gtgatcaggt
    29281 tttctccgac ttctaacgtt tgtaataaac cggattgatg aataatggag atcacttttc
    29341 gcgcctgcca gagggagagt cggggacttt tgcgcaacgt taaatagcaa aaacctaaac
    29401 tgatgccact actgacctgg ctgacccgtt gcagctcctc cggtttgccg atggtgattt
    29461 gcacgggatg ccgacaactg gttcccaaat ctttttcgaa aagcggcgga gttatccagt
    29521 actcaaccgc tttaagcagc ggatgacgta aatggtaata atcatggctt tccaggcgca
    29581 ccagttcagc tgtgaattgt gttaagcgaa aaggacccgt gccaatcagt gggaattgcg
    29641 gatgcgccag atggctgcaa tagctcgcca gccggtgcgc aagccagtaa tcggggcgat
    29701 gtaaaaagaa ggtcagacac tgcggatggg tgacctcaat acgcttcacg ctaataaata
    29761 attgatccag tgctggcagt tgtaacagca tcaataatcg ctggtgtaag tgtgaggctt
    29821 ttactgcatc gccgttatgc cagtgtaggg ttgaacgaag ataaaagtcc cagcgtaacc
    29881 cgtcagcaga ggtttcccag tgatgcgcta aatcgccaat cgggcgctgg gtattattat
    29941 cgaagcgggt caggccggaa aatatctgtc cggcgagatg ctgctcggca cggccgggca
    30001 aaaagcccgg ttgtagcggt tcgagcgggc gatagtaggg aatacgcaac gtgggtgtat
    30061 cgttttgcca ttgtccgccc ataaacggct gtaacagagt gcgcagctca cctggggcca
    30121 gttgcgccag ctccagcaca tcttgctgct ttccggtttc cagtgccttt tccatcatcg
    30181 cattgcgtag cgattctggc gtgaccagaa agcgtaattg tccgcgcttt ccgcgtcctg
    30241 actgcgcctg ccactccagc catcccgctt cctgtgcctg acgcaacagc gtacgaacat
    30301 ggcgttcgct gcaaaaacag cgttcggcca gttcgctgac ggtgacggtt tgcggctttc
    30361 cggcggaagg ttgccacaga cgttgatact ggttaaggcg gttgagcagt cgcataaaaa
    30421 cccggaacaa tattatttaa ctattcacta ttacttccgt atatatcagg tgatactcaa
    30481 tcaccattaa ccgtgtcaca gagtggagaa agaaatggct cgtctggcag catttgatat
    30541 ggatggcact ttattgatgc ctgaccatca tttaggtgag aaaaccctct ctactttggc
    30601 gcgactgcgt gaacgcgaca ttaccctcac ttttgccacg gggcgtcatg cgctggagat
    30661 gcagcatatt ctcggggcga tatcgctgga tgcgtatttg attaccggca acggaacgcg
    30721 cgtgcattct ctggaaggtg aacttttaca tcgtgatgat ttacctgcgg atgtcgcgga
    30781 gctggtgctg tatcagcaat gggatacccg agccagcatg catattttca atgacgacgg
    30841 ttggtttacc gggaaagaga tccctgcgtt gttgcaggca tttgtctata gcggttttcg
    30901 ttatcaaata atcgatgtca aaaaaatgcc actcggcagc gtcaccaaga tctgcttctg
    30961 tggcgatcac gacgatctta cacgtttgca gatccagcta cacgaagcat taggcgagcg
    31021 tgcacatttg tgtttttccg ccacggattg ccttgaagtg ctgccggtgg gctgcaataa
    31081 aggcgctgca ctgacggtgc tgacccaaca tttaggttta tcgttgcgcg attgcatggc
    31141 ctttggtgat gcgatgaacg atcgcgaaat gttaggcagc gtcggtagcg gatttattat
    31201 gggcaatgcg atgccgcaac tgcgcgcgga gctcccgcat ttaccggtga ttggacattg
    31261 ccgaaatcag gctgtctctc actatttgac gcactggctg gactatccac atctacctta
    31321 ttcccccgaa taacgagatc ccttccagca ccgggcaatt gcccggtttt tttgcgttga
    31381 atttgtcatt ttgtgccgtg gtgtttaaac cgcacagaat aaattgtcgc gatttcacct
    31441 ttaaaataga attaaaagag aaaaaaattc tccgtggaag ggctatgtta gataaaattg
    31501 accgtaagct gctggcctta ctacagcagg attgcaccct ctctttgcag gcactggctg
    31561 aagccgttaa tctgacaacc accccctgct ggaagcgcct gaaacggctg gaggacgacg
    31621 gtatccttat cggcaaagtc gccctgctgg atccggaaaa aataggcctc ggcctgaccg
    31681 cctttgtgct gataaaaacg caacatcaca gcagcgaatg gtattgccgc tttgtcacgg
    31741 tggttaccga aatgccagaa gtgctggggt tctggcgcat ggctggtgaa tacgattatc
    31801 tgatgcgcgt ccaggttgcc gacatgaaac gctacgacga gttttataag cgtctggtaa
    31861 acagcgtgcc ggggctgtcg gacgtcactt ccagcttcgc gatggaacag attaaataca
    31921 ccacttcttt acccatcgaa taaatatcca gaatcaggtc aggacacaac gcgtgcgatt
    31981 atttgctcaa ttaagctggt atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc
    32041 cttgcttgtc attatcgcga tgctgcaact ggttccacca aaagtggttg gtattgttgt
    32101 cgatggcgtg acagaacaac actttactac cgggcagatc ctgatgtgga tcgccaccat
    32161 ggtgctgatt gccgttgtgg tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg
    32221 tgcgtcttat caactggctg ttgaactgcg tgaagattat taccgtcagc taagccggca
    32281 gcatcctgag ttttacctgc gtcatcgcac gggtgacctc atggctcgtg cgactaatga
    32341 cgtcgatcgc gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg attcactggt
    32401 aatgggctgc gctgtgttga ttatgatgtc tacgcaaatt agctggcagt tgaccttatt
    32461 tgccctgtta ccgatgccag tgatggcgat catgattaag cgcaacggcg atgctttgca
    32521 tgaacgcttt aagctggcac aggcggcgtt ttccagtctt aatgaccgca cccaggaaag
    32581 cctcaccagt atccgcatga tcaaagcctt tggtctggaa gatcgccagt cggcgttatt
    32641 tgccgcggat gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg
    32701 tttcgacccg accatctata tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg
    32761 tagttggatg gtggtgcagg gcagtttaac gctgggccag ctcaccagtt ttatgatgta
    32821 tttaggtctg atgatttggc caatgctggc gctggcatgg atgtttaaca ttgtggaacg
    32881 tggtagtgct gcgtacagcc gtattcgcgc gatgctggcg gaagcgccgg tggtgaacga
    32941 tggtagcgaa cccgtgccgg aagggcgtgg cgaactggat gtaaatattc accagttcac
    33001 gtatccgcag actgaccatc ctgcgctgga aaacgtcaat ttcgccctga aacccggtca
    33061 gatgctgggt atctgcgggc cgactggttc cggcaaaagt accctgttgt cgctcattca
    33121 gcgtcatttc gacgtcagcg agggggatat tcgctttcat gatattcctc tgacgaagtt
    33181 acaactcgat agctggcgta gccgcctggc ggtagttagc cagacgccat tccttttttc
    33241 tgacaccgtg gcgaataaca tcgcgctggg ttgcccgaat gccacccagc aagagattga
    33301 gcatgtcgcg cggttagcca gcgtacatga cgatattttg cgtctaccgc aaggttacga
    33361 tacagaggtg ggcgagcgcg gtgtgatgct ttccggcggg caaaaacagc gtatctccat
    33421 tgctcgtgcg ttattagtca acgcggaaat cctcatcctt gatgatgcgc tttcggcggt
    33481 ggacggacgc actgagcacc agatcctgca taacctgcgt cagtgggggc agggaagaac
    33541 ggtaatcatc agtgcccatc gcctttctgc actgacggaa gccagtgaaa ttattgtgat
    33601 gcagcacgga catatcgccc agcgtggcaa tcatgatgtg ctggcacaac aaagcggctg
    33661 gtatcgcgat atgtatcgct atcaacaact ggaggcggcg ctcgacgacg ctccggaaaa
    33721 tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg gccgactctc aagcgcctgt
    33781 tagcgtacgg ttcaccgtgg cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg
    33841 cggcggcggc agaagtcagt gggccgctgc ttatcagcta ttttatcgac aatatggtag
    33901 cgaaaaataa cctgccgttg aaagtggttg cagggctggc tgcggcgtat gttgggctgc
    33961 aactgtttgc cgccgggcta cattacgcgc agtcgctgct gtttaatcgg gcggcagtag
    34021 gcgtagtgca acagttgcgt accgacgtga tggatgctgc gttacgccag ccattaagcg
    34081 agtttgatac ccaacccgtc gggcaggtga tttcccgcgt cactaatgac actgaagtga
    34141 tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg cagtgccgca ctggtgggcg
    34201 cgatgctggt ggcgatgttc agcctcgact ggcgaatggc gctggtggcg ataatgattt
    34261 tcccggtggt gctggtggta atggtgatat accagcgtta cagcacgcca attgtccgtc
    34321 gtgtgcgcgc ctatctggcg gatatcaacg acggctttaa cgaaatcatc aatggcatga
    34381 gcgttatcca gcagtttcgt cagcaggcgc gatttggcga acgtatgggt gaggccagtc
    34441 gttcacacta tatggcgagg atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc
    34501 tgctgagtct gttttcatcg ctcattctct gtggcttgtt gatgctgttt ggcttctccg
    34561 ccagcggcac cattgaagtg ggcgtgttgt atgcgtttat cagctatctt gggcgactta
    34621 acgaaccctt aatcgaactg accacgcagc aggcgatgct gcaacaggct gttgttgctg
    34681 gtgagcgcgt gtttgagctg atggacggac cgcgccagca atatggcaat gatgatcgcc
    34741 cgttacagag tggcaccatc gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc
    34801 tggtgctaaa gaacattaat ctctctgtgc cttcgcgtaa ctttgtggcg ctggtcgggc
    34861 ataccggcag tggtaaaagt accctcgcca gtttattgat gggctattac ccgctaacgg
    34921 aaggtgagat tcgccttgat ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc
    34981 agggcgtggc aatggtgcag caagatccgg tggtgctggc ggataccttc ctcgccaacg
    35041 tgacgctggg gcgggatatc tccgaagaac gcgtctggca ggcgctggaa accgtgcaac
    35101 tggcggagct ggcacgcagt atgagcgacg ggatttatac gccgctgggc gagcagggga
    35161 ataatctctc tgtcgggcaa aagcaactgc tggcactggc gcgcgtgctg gtcgagacgc
    35221 cgcaaatcct gatccttgat gaggcaaccg ccagcattga ctccggtact gaacaggcga
    35281 ttcaacatgc tctggcggcg gtgcgtgaac ataccacgct ggtagtgatt gctcaccgct
    35341 tatcgactat tgttgatgcc gacactattc tggtgcttca tcgtgggcaa gccgtggagc
    35401 agggcactca ccagcaactg ctggcggccc agggacgcta ctggcagatg tatcaactgc
    35461 aacttgcggg cgaagagctt gcagccagcg tgcgtgaaga agaatcattg agcgcctgaa
    35521 tagcgcaata tctcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt
    35581 tttcattttc aatcttctta acttcctgct ctctttctcg tttttcattt ctggcacacc
    35641 gcttgcaata ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg aggggatcta
    35701 tgaagctggt gaccgtgata atcaaaccat tcaagctgga agacgttcgt gaagcgttat
    35761 cttccattgg tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag
    35821 ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa
    35881 ttgatgtggc gattgctgat gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg
    35941 cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc
    36001 gtattcgtac cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac agcaacagga
    36061 acgaaaaatg aagatagcga cgataaaaac tgggcttgct tcactggcga tgctgccggg
    36121 actggtaatg gctgcacctg cggtggccga taaagccgac aatgcgttta tgatgatttg
    36181 tactgcactg gtgctgttta tgactattcc ggggattgcc ctgttttacg gtgggttgat
    36241 tcgcggcaaa aacgtgctgt cgatgctgac gcaggtgacg gtgacgtttg cactggtctg
    36301 tattctctgg gtggtttacg gttactcgct ggcgtttggt gagggcaaca acttcttcgg
    36361 caacattaac tggttgatgc tgaaaaacat cgaactgacg gcggtgatgg gcagcattta
    36421 tcagtatatc cacgtagcgt ttcagggatc gtttgcctgc attaccgtag gcttgatagt
    36481 tggggcgctg gcggaacgaa tccgcttctc agctgtgttg attttcgtgg tggtatggct
    36541 gacgctctct tacattccta ttgcgcatat ggtgtggggc ggtggtttgc tggcttctca
    36601 cggtgcgctg gatttcgcgg gtggcacggt ggtgcatatt aacgccgcaa tcgccggtct
    36661 ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca
    36721 caacctgccg atggtcttca ccgggactgc cattctctat atcggttggt ttggctttaa
    36781 cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga atactgtggt
    36841 cgcaacggcg gcggcaattc ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc
    36901 ttcactgctg ggagcgtgtt ctggcgcgat tgccggtctg gtcggcgtga cgccagcgtg
    36961 tggctacatt ggtgttggcg gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt
    37021 gtggggcgtt accatgctca aacgcttgct gcgggtggat gatccctgcg atgtcttcgg
    37081 tgtgcacggc gtttgtggca ttgtcggctg tatcatgact gggatttttg ccgccagctc
    37141 gctgggcggc gtgggcttcg ctgaaggtgt gacgatgggc catcagttgc tggtacagtt
    37201 ggaaagcatt gccattacga ttgtgtggtc tggtgtggtg gcatttatcg gctacaaact
    37261 ggcggatctg acggttggtc tgcgtgtgcc ggaagaacaa gagcgagaag ggctggacgt
    37321 caacagtcac ggcgagaatg cctataacgc ataacaagca ctgcaaaaaa cagccggacg
    37381 gttttcacct ccggctattt ttttaattgt gattacgcat caccccttcc tgaacggtcg
    37441 aggcaaccag tacgccgtcc tgggtataaa actcaccgcg cacaaagcca cgtgcgctgg
    37501 acgccgaggt gctctccacg ctatacagca gccattcatt caaattaaac gggcgatgga
    37561 accacatgga atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa ccgataccgt
    37621 gcggctgtag agctaccggc aggaagttaa gatcagaagc gtaaccgagc agatactgat
    37681 gaacgcgcag gtcatccggc acgctaccat ttgcgcggat ccacacctga cgatgtggtt
    37741 ctgcgacgtg acctttcagt gggttatgaa actccaccgg acggacttcc agcggacgat
    37801 cgcagatgaa tttatcttta agcactggcg gcagcaggtg cgccagcgat tgggcgattt
    37861 gcgtttccga aggcaggcca tcaggcgctg gcgcggacgg cattgttttt tgatgttcga
    37921 aacccgcttc tggtgcctgg aaagaggccg tcatataaaa aatcggtttg ccgttttgaa
    37981 tagcagcaac ccggcgggcg ctgaagctgt taccgtcacg cagcgtttcg acatcataaa
    38041 taatcggctt cttactatcg ccagggcgaa gaaagtagct gtgaaacgaa tgtaccagcc
    38101 gctcttcagg gacggtctct tttgcagcat acaaggcctg acccacgacc tggccgccaa
    38161 atacctggcg taaacctaaa tcttcactct ggccgcgaaa gagtccttcc tcaatttttt
    38221 ccagatttaa caatgtcagt aaatttttta gcgcctgact catataactc tccagtaaca
    38281 aagctgccgc agcaagccaa agtgagttga gtataacgca aatttgctac tggtccgatg
    38341 ggtgcaatgg tctgaattac gggctaatta caggcagaaa tgcgtgatgt gtgccacaat
    38401 tgttgacgtt actattttgt taaccactct tccggcgagg aaagttagcc cgctggtgca
    38461 ttgataataa ggagaaatga atgaaactcg tgcacatggc cagtggttta gcggttgcga
    38521 ttgcgttggc ggcttgcgca gataaaagcg cggatattca gacgccagcc ccggctgcaa
    38581 atacgtctat ttcagcaaca caacaacctg ctatccagca accgaatgtc tccggtaccg
    38641 tctggatccg tcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg acactttctg
    38701 acgcgtcgtt agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag
    38761 gtaaacagtc accattcagc tttgttctgc catttaaccc ggcagatgtt cagccgaacg
    38821 cgcgtattct gttgagtgcg gcgattaccg tgaatgacaa actggtattt attaccgata
    38881 ccgttcagcc ggtgatcaac caggggggaa ccaaagccga cctgacattg gttccggttc
    38941 agcaaaccgc cgtgcctgtc caggccagtg gtggcgcaac aactaccgta ccatcgactt
    39001 caccgaccca ggtgaatcca tcttcggcag ttcctgcccc tacgcaatac taagcgttac
    39061 taaccccttt ccatctggag aggggttagt agttccagcg atagcgctgt aagtcgattt
    39121 gcccgcttcc cgataccatc acaccttctg ccagtaatgc ctgtcgttga cgctgtaaat
    39181 ccggtccggt tagcgaaatg gtgccgtggc gattaaccac ccggtgccag ggcaaggtgc
    39241 tgccttcggg gagacgcttt aacacaccgc caacctggcg cgcggcgcgg ggcgatcccg
    39301 ccagtttcgc cacatcaccg taagtggtga catacccttc gggaatagcg gctacgattt
    39361 gccagacgcg ttggggaaat gaatcttctt tttccatctt ttcttcctga gataattttt
    39421 cagcataatc tggaaaaacg cccgagtgaa gccgcattgc gcaagaaacc agcatctggc
    39481 acgcaatggg ttgcaattag ccggggcagc agtgataatg cgcctgcgcg ttggttctca
    39541 acgctctcaa tgggggctct gttggttctc ccgcaacgct actctgttta ccaggtcagg
    39601 tccggaagga agcagccaag gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc
    39661 cacccatttc tgcctcccac cgtttcgtca aaaaatccca acatgactaa actttaacca
    39721 caactgacgt cgcaagaatt gtctggctgc gcagtacgct tcggaggtat gtctgatgaa
    39781 gtatgttgat ggttttgtgg ttgccgttcc tgccgataaa aaggatgcct atcgggaaat
    39841 ggccgctaag gccgcgccat tgtttaaaga gtttggcgcg cttcgtattg tcgaatgctg
    39901 ggccagcgat gtaccggatg gcaaagtgac cgattttcgt atggcggtga aagcggaaga
    39961 gaatgaagag gttgtcttta gctggattga atacccttca aaagaggtcc gcgacgcagc
    40021 taatcaaaag atgatgtcgg acccacggat gaaagagttc ggcgagtcca tgccgtttga
    40081 cggcaagcga atgatctatg gcggattcga gtcaatcatc gacgaatagg tagcgtgacg
    40141 ggctgtgcgg cgtgccgtca gcccaaaaat tcacaaatgt tgctcggccc agcgtaagaa
    40201 atcttctttc ggtaatgcct tgctgtagag ccagccctga ccgtagtgca cgccatgctg
    40261 gcgtagccac tcttcttgtt tactggtttc gattccctcc gctaccattt tcagtttcag
    40321 tgtttttgcc atttcgatga tatgcggcgt gacattttta tattccagcg catcaacgaa
    40381 agatttatcg atcttcagaa tgtcgacatc cagatcctgt aaataactta aacttgaata
    40441 ccccgtacca aaatcatcaa gataaatttc atggcccgcc tcccggtagc gagaaattat
    40501 cggggcgctg gttttcggat cggcaaactc gcgttcagtg agttcaagcg cgatctgtct
    40561 gggattaacc tgatagtgat tgatcatttc acgcagcaat tgcgggattt tttccgaggt
    40621 gagcacggtg gattcaagat tgatcgaaat atgctgctgt ggatgctgac gcagccagtc
    40681 gcccatatct tcaaagacgc ttcttataat cagtagcgtc aatggctcag aaaggcccgt
    40741 ttgctgtgcc agcggaataa aactatctgg tgacaaccaa ctaccgtctg tctgcggcca
    40801 gcgcgccagt gcctcagcac cgacaatttt gccattggct aaggagacaa tcggctgata
    40861 gtgcacgcaa atatcacgat tttcgatagc atcctgcagc cgatgatgcg gtgactggat
    40921 gcggcgcaaa atgcgcagca caaacatcgc tgccagcagg ccaatcacca acccgagcgg
    40981 taaccaaata aagacttgcc gatgccagcc tttctgcaac attttcgatg aagcccaggt
    41041 gataattgaa atattcatct ccgggaaggg caggatatcg taaatgattc cattattttc
    41101 gatatgctca ccgggtgttt tttgtagcct ggtaataatt ccctgagcaa tttcatcgct
    41161 gctggttatg acaacgttat gggcattgcc aataatggcg gcatcaattt gccatgagct
    41221 atagggaatg acatcaataa aggaagcggg gtcgatcatg acgacataat gtgccgttcc
    41281 catggcgacc atgtaacgga taatgcctaa atcgttatgt gatgttaacc agacacgata
    41341 accatctttc gaaattttac ctggctcggg gaaggtatcg ggcggacttt catgctccag
    41401 agacgaacac tggggaacgt tgttatcgat ataaaccact tcctgaatat agcgataact
    41461 gtaagatacc cgacgcattt ccatgagatg ggcttcacta cagtcagcgc cttgccatct
    41521 ttccagctct tgcagcgcat ctttcccttg tgtcgccacc ttattggccc gaatagcgac
    41581 gcgggaggaa taggtatcca gctcttcaat aaacgatgtt tctacctgct gatgggccag
    41641 ccagatgctt aagccgacag gcagcaatac tgaaagaatc agtactcccg aaataaggcc
    41701 gaccagatgt cgtgttctca ccagcatatc cttattaaca cagtgtaggg aaagtatatc
    41761 tgattgtgga aaaaggctgg caggagataa ggaggtagag ctggtatttc atgaagtgat
    41821 gggtggaaac gatcgttgtc atttgaccag gcgcagagct ttacgcctgg tatgtaatcc
    41881 taacttaaga tgttgattcg gcgcgagcga gggtaaaaat atcgtaaaaa gacaattcac
    41941 ctttacggac gatcattttt tgcagttgtt ccttatgaac atccgcgaca cgcggagagt
    42001 tcaagatggc ttcaaccagc gcggcaggta caaagcgcgt gttgtaccat tcgccgttat
    42061 agcaaacgcg gaaatccaac acgtcgatat cttcatagcg gtagcgtggg tagacatcga
    42121 aaaagaagaa accattaagc aggataaata atacaaccag tagccagaca gagcctgaaa
    42181 gcgtttcgga ctgcaacatc accgccaggg tggcaaaaaa agcaacgtac ataccgataa
    42241 acagccccgg atgtttacgg ataaaactga tactaaagcg gggtttgttg tcgcgttttt
    42301 cgcgggtatt cagagactca atcgtttcgg tcagcaggcg ttgtatttcg gtcatcgttt
    42361 gccttcgtgg tatctgcaac tttccaggga acaccctatt ttaacgtggg aatagtagag
    42421 ggaaagtaac agatctgcca caattttgca taacagttgc gaaaaaccga ttacaatttt
    42481 ttaattattc tggctggatt accgcccacg acaacgttgt ccgggacatc ttttgtgaca
    42541 actgcacctg aggctaccac gacgttatca ccaatggtca caccagggtt aatgaccgcg
    42601 cgtccgccaa tccagacgtt attaccgatg gtgacgggtt tccccagttc agcaccgcta
    42661 ttacgtgcta cagggtcgat gggatgtgtt gccgtgtaga tatgaacgcc tagtgccaac
    42721 atacagttat caccgatgcg aatagggcag acatcaagca tcacgcaatc gaagttggcg
    42781 aaaaaattat taccgagaaa aatgttatag ccatagtcac agcgaaacgt tggctcaata
    42841 taagcctctg tcacctgacc gaatagatca gcgagaattt gctggcgtaa tgtgtgctct
    42901 tccgccaggg aatgattgta tcggtgaata agctgacgag cgcgcaggcg atcgcgagat
    42961 aacgtctcat ctgccgagcg atacaactca ccagcaatca tcttttcttt ttctgtgctc
    43021 attactgacc tccggttgct gaaaatgcgg caacacagta agacgaaaag tcagtgaaga
    43081 gaacgttccc attacatctg tgtgataaag atcacatagg gtatgtcttc atggcgaaaa
    43141 agtataaaat tcttaataaa cagccggtta tagctccgaa agcgaattat ttattagcga
    43201 ataaatttcc atactgagga agggatcttg tcgtacagtt tattcatggt caattcggcg
    43261 aggcggtgat ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta
    43321 tttttctcga taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa
    43381 taatcggttt tcgttaaagg tttttcggac atacttctac ccatggttgt aataattcta
    43441 acaagataaa ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt
    43501 acctgacttc cgccatttct gaagatcctg catattaata ccataactac tgaacaacat
    43561 aaaggtgtca tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt
    43621 aattttgtaa ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt
    43681 gatcgccgag gttgggtcgt taacccagcc atgattgctt tcttcaaggt ttgcaaggca
    43741 gtcatgatac agagtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga
    43801 gtattcatcc ataacgcgtc cccttcttag cggttgaact aacggacacc tttcgggatg
    43861 gaaaaaactt actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct
    43921 ttgcaggttt tcatcgcatt attacgaaaa attacaatta ggaaggaagc gggacagggc
    43981 tcactttttc atactagcac caacccgcag caggtaaaag cagtttgctc gaactgttca
    44041 taacatttca ggattatcgc cagatgaaat tggtgacact aataccagga ttgctctgaa
    44101 tatgacgtaa taaccgagga atgaataaag aattaccgca aataattaag aatagcctct
    44161 aaatgat
//
LOCUS       DAZFKY010000017        42258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFKY010000017 DAZFKY010000000
VERSION     DAZFKY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..798
                     /gene="ydhY"
                     /locus_tag="ACHKCL_000857"
     CDS             172..798
                     /gene="ydhY"
                     /locus_tag="ACHKCL_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5761884.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            819..2921
                     /gene="ydhV"
                     /locus_tag="ACHKCL_000858"
     CDS             819..2921
                     /gene="ydhV"
                     /locus_tag="ACHKCL_000858"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5761885.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2934..3572
                     /gene="ydhW"
                     /locus_tag="ACHKCL_000859"
     CDS             2934..3572
                     /gene="ydhW"
                     /locus_tag="ACHKCL_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5761886.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3636..4304
                     /gene="ydhX"
                     /locus_tag="ACHKCL_000860"
     CDS             3636..4304
                     /gene="ydhX"
                     /locus_tag="ACHKCL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5761887.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4301..5086
                     /gene="phsC"
                     /locus_tag="ACHKCL_000861"
     CDS             4301..5086
                     /gene="phsC"
                     /locus_tag="ACHKCL_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5761888.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHKCL_000862"
     CDS             5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHKCL_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5761889.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5914..7518)
                     /gene="ydhS"
                     /locus_tag="ACHKCL_000863"
     CDS             complement(5914..7518)
                     /gene="ydhS"
                     /locus_tag="ACHKCL_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5761890.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7644..7949)
                     /gene="ydhR"
                     /locus_tag="ACHKCL_000864"
     CDS             complement(7644..7949)
                     /gene="ydhR"
                     /locus_tag="ACHKCL_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5761891.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8057..8133)
                     /locus_tag="ACHKCL_000865"
     tRNA            complement(8057..8133)
                     /locus_tag="ACHKCL_000865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8097..8099),aa:Val,seq:gac)
     gene            complement(8138..8214)
                     /locus_tag="ACHKCL_000866"
     tRNA            complement(8138..8214)
                     /locus_tag="ACHKCL_000866"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8178..8180),aa:Val,seq:gac)
     gene            8522..9778
                     /gene="ydhQ"
                     /locus_tag="ACHKCL_000867"
     CDS             8522..9778
                     /gene="ydhQ"
                     /locus_tag="ACHKCL_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5761892.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9819..11192)
                     /gene="mdtK"
                     /locus_tag="ACHKCL_000868"
     CDS             complement(9819..11192)
                     /gene="mdtK"
                     /locus_tag="ACHKCL_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5761893.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            11407..12048
                     /gene="ribE"
                     /locus_tag="ACHKCL_000869"
     CDS             11407..12048
                     /gene="ribE"
                     /locus_tag="ACHKCL_000869"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5761894.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(12088..13236)
                     /gene="cfa"
                     /locus_tag="ACHKCL_000870"
     CDS             complement(12088..13236)
                     /gene="cfa"
                     /locus_tag="ACHKCL_000870"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5761895.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13527..14738)
                     /gene="punC"
                     /locus_tag="ACHKCL_000871"
     CDS             complement(13527..14738)
                     /gene="punC"
                     /locus_tag="ACHKCL_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5761896.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14851..15783
                     /gene="punR"
                     /locus_tag="ACHKCL_000872"
     CDS             14851..15783
                     /gene="punR"
                     /locus_tag="ACHKCL_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5761897.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15780..16805)
                     /gene="purR"
                     /locus_tag="ACHKCL_000873"
     CDS             complement(15780..16805)
                     /gene="purR"
                     /locus_tag="ACHKCL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5761898.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17104..17193
                     /gene="cydH"
                     /locus_tag="ACHKCL_000874"
                     /gene_synonym="ynhF"
     CDS             17104..17193
                     /gene="cydH"
                     /locus_tag="ACHKCL_000874"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5761899.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17359..18528
                     /gene="ydhP"
                     /locus_tag="ACHKCL_000875"
     CDS             17359..18528
                     /gene="ydhP"
                     /locus_tag="ACHKCL_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761900.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18674..19255)
                     /gene="sodB"
                     /locus_tag="ACHKCL_000876"
     CDS             complement(18674..19255)
                     /gene="sodB"
                     /locus_tag="ACHKCL_000876"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5761901.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19383..20198)
                     /gene="mepH"
                     /locus_tag="ACHKCL_000877"
     CDS             complement(19383..20198)
                     /gene="mepH"
                     /locus_tag="ACHKCL_000877"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5761902.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SLTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20533..20880
                     /gene="grxD"
                     /locus_tag="ACHKCL_000878"
     CDS             20533..20880
                     /gene="grxD"
                     /locus_tag="ACHKCL_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5761903.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20931..25547)
                     /gene="lhr"
                     /locus_tag="ACHKCL_000879"
                     /pseudo
     CDS             complement(20931..25547)
                     /gene="lhr"
                     /locus_tag="ACHKCL_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(25640..26287)
                     /gene="rnt"
                     /locus_tag="ACHKCL_000880"
     CDS             complement(25640..26287)
                     /gene="rnt"
                     /locus_tag="ACHKCL_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5761904.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26390..26797)
                     /gene="gloA"
                     /locus_tag="ACHKCL_000881"
     CDS             complement(26390..26797)
                     /gene="gloA"
                     /locus_tag="ACHKCL_000881"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5761905.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26878..27975)
                     /gene="nemA"
                     /locus_tag="ACHKCL_000882"
     CDS             complement(26878..27975)
                     /gene="nemA"
                     /locus_tag="ACHKCL_000882"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5761906.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28012..28611)
                     /gene="nemR"
                     /locus_tag="ACHKCL_000883"
     CDS             complement(28012..28611)
                     /gene="nemR"
                     /locus_tag="ACHKCL_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5761907.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28714..28953
                     /gene="ydhL"
                     /locus_tag="ACHKCL_000884"
     CDS             28714..28953
                     /gene="ydhL"
                     /locus_tag="ACHKCL_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5761908.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29002..29898
                     /gene="ydhF"
                     /locus_tag="ACHKCL_000885"
                     /pseudo
     CDS             29002..29898
                     /gene="ydhF"
                     /locus_tag="ACHKCL_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            29979..30500
                     /gene="sodC"
                     /locus_tag="ACHKCL_000886"
     CDS             29979..30500
                     /gene="sodC"
                     /locus_tag="ACHKCL_000886"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5761909.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30501..32513)
                     /gene="ydhK"
                     /locus_tag="ACHKCL_000887"
     CDS             complement(30501..32513)
                     /gene="ydhK"
                     /locus_tag="ACHKCL_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5761910.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32513..33370)
                     /gene="ydhJ"
                     /locus_tag="ACHKCL_000888"
     CDS             complement(32513..33370)
                     /gene="ydhJ"
                     /locus_tag="ACHKCL_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5761911.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33373..33609)
                     /gene="ydhI"
                     /locus_tag="ACHKCL_000889"
     CDS             complement(33373..33609)
                     /gene="ydhI"
                     /locus_tag="ACHKCL_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5761912.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33810..34244
                     /gene="slyA"
                     /locus_tag="ACHKCL_000890"
     CDS             33810..34244
                     /gene="slyA"
                     /locus_tag="ACHKCL_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5761913.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34291..34758)
                     /gene="slyB"
                     /locus_tag="ACHKCL_000891"
     CDS             complement(34291..34758)
                     /gene="slyB"
                     /locus_tag="ACHKCL_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5761914.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35031..36140
                     /gene="anmK"
                     /locus_tag="ACHKCL_000892"
     CDS             35031..36140
                     /gene="anmK"
                     /locus_tag="ACHKCL_000892"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5761915.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            36238..36567
                     /gene="mliC"
                     /locus_tag="ACHKCL_000893"
     CDS             36238..36567
                     /gene="mliC"
                     /locus_tag="ACHKCL_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5761916.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36626..37282
                     /gene="pdxH"
                     /locus_tag="ACHKCL_000894"
     CDS             36626..37282
                     /gene="pdxH"
                     /locus_tag="ACHKCL_000894"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5761917.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37411..38685
                     /gene="tyrS"
                     /locus_tag="ACHKCL_000895"
     CDS             37411..38685
                     /gene="tyrS"
                     /locus_tag="ACHKCL_000895"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5761918.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38747..39607
                     /gene="pdxY"
                     /locus_tag="ACHKCL_000896"
     CDS             38747..39607
                     /gene="pdxY"
                     /locus_tag="ACHKCL_000896"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO5761919.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39651..40256)
                     /gene="gstA"
                     /locus_tag="ACHKCL_000897"
     CDS             complement(39651..40256)
                     /gene="gstA"
                     /locus_tag="ACHKCL_000897"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5761920.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40362..41864)
                     /gene="dtpA"
                     /locus_tag="ACHKCL_000898"
     CDS             complement(40362..41864)
                     /gene="dtpA"
                     /locus_tag="ACHKCL_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5761921.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10643 a        10405 c        11040 g        10170 t
ORIGIN      
        1 tttctcctaa ccataaagga ttagtttatc ggcgttaaat attttctcat acactatcgg
       61 gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg aatgcttaat
      121 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      181 gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg tatatcagga
      241 aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      301 gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt gctggtgacc
      361 cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      421 ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt ctttggcgac
      481 aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      541 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      601 cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag cgcctgtact
      661 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      721 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      781 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat gactaacggt tggacaggta
      841 atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta
      901 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
      961 gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat
     1021 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1081 atttagtcgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggat
     1141 acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag attaaagatg
     1201 acaaagttag cctggaaaaa gcagatttct tatggggaaa agggacgcgc gcaacgacgg
     1261 aagaaatttg tcgcttgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1321 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc ggtgcgggaa
     1381 ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1441 tcaacattgc cgatcgtcag gagatgaagc gtctcaatga ttacatgatg actgaactta
     1501 tcggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag
     1561 atcccaagtc acgctggaca gcacgtaaag ggctgttttg gggcgcggct gaaggcggtc
     1621 caattgaaac gggtgaaatt ccgccaggca atcagaatac ggtcggcttt cgtacctata
     1681 aatccgtttt tgacttaggt ccggcagcag agaaatacac agtaaaaatg agcggctgcc
     1741 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1801 tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc atcttcccga
     1861 acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac ctggttggtc
     1921 tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat cgcgacttta
     1981 cttattgtta cagcaaaggt gtgttcaagc gtgttctgcc tgctgaagag tatgcagaaa
     2041 ttcgctggga tcaactggaa gcgggagacg ttaacttcat taaagatttt tactaccgtc
     2101 tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaat
     2161 gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg tcgccgtttg
     2221 gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt gttaactgta
     2281 tgttcaaccg tgactgcatg acgcataccc atatcaactt tattggttcc ggcttgccat
     2341 tgaaactgca acgtgaagtg gcgaaagaac tttttggttc tgaagatgct tacgatgaaa
     2401 ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg tcgctgttgc
     2461 gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc
     2521 cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc ttcaaagcga
     2581 tcactggcga agagatgact caggaaaaat tagatttagc tgcagagcgt atctttacat
     2641 tgcatcgtgc ctacacggtg aaactgatgc aaaccaaaga tatgcgtaat gaacacgatc
     2701 ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtactg
     2761 acaaaatgga tcgtgatgat atgcatgcct cgctcacgat gttctacaaa gaaatgggct
     2821 gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg ctggaagata
     2881 ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt aagatgaacc
     2941 atcaggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag cggcaatggt
     3001 tgcaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct gaaatcctgg
     3061 cagagttcat tcgccagcat tctgtggctg ggcagctggt ggcgcgcgcc gtgtttctct
     3121 cgccacccta ttcagttgca gaagaggaac tgtcagtttt gctggaaaac atcaagcaga
     3181 acggagacta cgcggatatc gcttgtatga cgggaagtca ggatgattac tattactcaa
     3241 cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt
     3301 gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt ccttacaaag
     3361 tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa gccgcgatcg
     3421 ccgcaatgga tgttgcacct gaatatgccg atatacggca ggtagagtca tcgacggcgg
     3481 tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc ttatgcgaat
     3541 ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt
     3601 ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg
     3661 ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca
     3721 cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct
     3781 gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgc ccccgctcag ggaagccgtt
     3841 tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc
     3901 gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg acaggggcgt
     3961 cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc ggctgcagtt
     4021 attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact aaagtggcag
     4081 ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca
     4141 gcgcttgccc ggaacacgca ttgatctttg gtcgcgaaga tagccctgaa attcaggcgt
     4201 ggttgcagga taataaatac tatcaatatc agctacctgg tgccgggaaa ccgcatctgt
     4261 atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc
     4321 tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg aattttggcc
     4381 ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc tggggctgca
     4441 cgccttgctt cgtgctcgtg gcgtgaagaa atcagccacc gatcatggtg agaggattta
     4501 tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt
     4561 gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg ttaaaagtct
     4621 ggttgcggtg catgaagttt gcggattttt gttactggca tgctggctcg gctttgttct
     4681 gatcaatggc gttgggggta atggccacca ctatcgcatt cgtcgtcagg gttggctgga
     4741 gcgggcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg aagaacatcc
     4801 tttcccggca acaacccagt ctaaatttaa tcccttacag caggttgcct atgttggtgt
     4861 catgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct atccgcaagc
     4921 cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt ttgctctggc
     4981 atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc
     5041 acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgatcattac
     5101 acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt tcctgcgcca
     5161 tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac
     5221 ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt
     5281 cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag agagagcatt
     5341 ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataacgc
     5401 cagaatcgca accgctgggt atgctgcaca aattgccagt atgggttata gcgtgcggat
     5461 tggaagtgtc ggctttaaca gtcacattgg cagctccggt gagcgcgcac gggttgctgt
     5521 aaccggcaat tcttcgcgga taagcagcgc tggagatagc agtcggattg cgaataccgg
     5581 aatgcgagta cgcgtttgta cattgggtaa acggtgtcat gtcgccagta atggtgacct
     5641 ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca
     5701 catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg actcaattat
     5761 tctcgggcca gggggcagcg ccgcactggc atatcatgac ggcgaaagag ttcgcttcgc
     5821 cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgtc ttaatgaaca
     5881 gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac gccgacgagc
     5941 acgggatgca ggctttacga ccgcccgagc catcgcctca ccaatttctg cacatgccgt
     6001 aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga acgctacgcg
     6061 cccacgaata tcttcgggtt gctgtaacgt ataatcttca ccaacatcag ggatttcatc
     6121 ccctgttttc tgtaattgtt cgcgtagtcc agggaaagga atatctttca ctttaagcgg
     6181 acgctgcccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt ccgttttcag
     6241 gacggtgcgc gactcgttaa tttccatttt gtagtcttca ccgagagcga gaatatgaat
     6301 gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga ttgccgcata
     6361 attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt gttcattcag
     6421 atgtggaaca atttcctgta cggcttcatg caggcgcaga atgacataac gccagggaac
     6481 agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg cccagtcaaa
     6541 ttggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga cattcaggct
     6601 ctctaaggct atgcgttgac tccagtctgg atcagcaaac ttgatttcct ctactatcaa
     6661 tctaaatacc cgatccaaaa ggccatattc gcctttttga atctcagcat ttaatgcctg
     6721 atcagtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg cttcgggtaa
     6781 aatacccgtg cgcgacatta acgtaatatt tagcttttca cttgcgttat cgcggtgaaa
     6841 aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca ctgccattgc
     6901 cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat cgacctttgc
     6961 ttccatcaag cctgaccacg ggcttggaaa atacgttcgg gttgcttctt cttcatcagg
     7021 ccagacgtga cccgtggcga tcaccgctaa atcaaacgtc tcgctgggta aatcctgatt
     7081 tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc atgattcata
     7141 aacagccact gcaaattttt gctttcgtgc ctggtctact aatcgtaaaa attgatcgcg
     7201 gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg tttctttttt
     7261 aacgccataa cgctggagat gactggcttc ttgcttttgt agccattcga gatacgtaca
     7321 ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg agttttcctc
     7381 atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag aaatagaaag
     7441 tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc cggcacccac
     7501 aattgcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt agtgttatca
     7561 gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg aaaagggcgc
     7621 taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg actcagtggc
     7681 tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagtttttt cagttgggca
     7741 gtgtgttttt ccagataggc aagggcgctt ttttcatcag tgaaaaggta gatcccaccg
     7801 gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg ttcctgatta
     7861 atcgactctg caagtggttt aagctgctca gccattgcgt cgccaaatgg gccattaaaa
     7921 gcaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac gcatacgaag
     7981 acagtgtaaa tcttttaacg tattggatac agagaagaag cagagggaaa aaggagaaga
     8041 ccagattaac aaaatctggt gcgtccgagt ggactcgaac caccgacccc caccatgtca
     8101 aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat tggactcgaa
     8161 ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga acgcaacgtt
     8221 gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag aggcaaaacg
     8281 taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc tatattttgt
     8341 tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa cccaataatt
     8401 cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg tggataaatc
     8461 ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa aggaataaat
     8521 catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg ttaagaccgg
     8581 cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag ctggcggaag
     8641 agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg gtgggatcgc
     8701 caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg ccattgccac
     8761 agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta cggttaatgg
     8821 ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt tgttgctgga
     8881 aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca ttctcgatca
     8941 agagggcggc ctgttggtta atgggacaac ctcagcggtc gtggtagatg aaggtggtga
     9001 attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg gcggcgtttt
     9061 tatgctggcg gggaaagcca atgatacgtt gcttgctgat ggcaccatga ataatctcgg
     9121 tggtgaagac tctgacacta ttgttgagaa tggagccatc tatcgtctgg ggacggatgg
     9181 tcttcagctc tacagttccg gtaagacgca aaacctgtcc gttaatgtgg gtggtcgggc
     9241 tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag tgatcctgtt
     9301 gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac cggttgaact
     9361 gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg gcgcagatct
     9421 gcaacaatca acgattactg tacagcaggg cggtgtattg attctcgacg gcagtacggt
     9481 aaaaggtgac agtgtcactt tcagtattgg taacatcaat ctgaatggcg gaaaactgtg
     9541 gctgatcact ggtgcggcaa cgcatgtgca gctgaaagtg aaacgcctgc gcggagaggg
     9601 agcgatttgc ctgcaaacca gtgcgaaaga aatttcacct gacttcatca atgtgaaagg
     9661 ggaagttaac ggggatatac gcgttcagat aacagatgcc agtcggcaaa ctctgtgtaa
     9721 cgcactgaaa ctacagccag acgaagacgg gattggcgca acgctccagc ctgcgtaaaa
     9781 acacaggcgc accccgtgaa ggttgcgcct tgtctttatt agcgggatgc tcgttgcaga
     9841 atgatggctg acggcagacg ttgcaggtac cgcatacgca gcatcatcat aatggctgcc
     9901 gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg ttcaacgacc
     9961 agatcggtca gagccagaat atagccgctt ggcaagccca gcacccagta agccgtaaag
    10021 gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact gccaatcacc
    10081 tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc cgccagcgtt
    10141 acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga aaccgtgaat
    10201 atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc agcggtttgc
    10261 gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac cgctgccgcc
    10321 agcgacattg gaagcacgaa cattagtgaa ctaaagttca gggcaatctg gtgtcctgcg
    10381 acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag tgtcacttca
    10441 aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat aaccgcagga
    10501 tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tcgagcgggc gcgtttaatg
    10561 taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac gccacaacca
    10621 acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt caccggaatg
    10681 ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc cagaccttca
    10741 cattggttac gggcaacctg gaagaaaaga tagcccggcg cgccccacag caacgcacgc
    10801 agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga gcggataatg
    10861 taacctgcat tccacagcac cagcataatg agaacggaaa caaaacccgc cagccagaag
    10921 ccctgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa ttgcgcgata
    10981 accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag ccagatagaa
    11041 gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat cacggtatcg
    11101 acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc taatgccaat
    11161 aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg tagttatatg
    11221 agagactaaa aagccgcctg aataggcagc aaaaagaaga agcaggggaa attcagtcta
    11281 ttgtagcgag gatttattaa ttctccagtg aaaaaatagc cattcttacc ccttcactgg
    11341 taagggcgtt ttccacctgc tattgtgctg aacagaatgt cttaactgat ttcaggagtt
    11401 gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat tgacgagaaa
    11461 ccaaattttc gcacacatgt ggtggagtta cccgaccaca tgctggacgg tctggaaacc
    11521 ggtgcctccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa cggcaaccat
    11581 gtcagttttg acctgatgaa agaaacgttg cgcattacca atcttggcga tttaaaagtg
    11641 ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg cggacactta
    11701 atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc ggaaaataat
    11761 cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct gtacaaagga
    11821 tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac gcgtttttgc
    11881 gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa acttggcgca
    11941 cgcgtcaaca ttgaaatcga tccacaaact caggcagtgg tagatacggt agaacgtgtg
    12001 ctggcggcac gagaaaatgc catgaatcaa tcaggtacag aagcctgatc tgaaatcgcc
    12061 cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt tttcaacacc
    12121 gcgtgagaac accacctgcc agagctgaat atcacgggcg cggaaagcac ctgcgcaggc
    12181 attcagataa taggtaaaca tccgtttaaa gcgttcactg tagttatccg caatttctgg
    12241 ccatgcggcg acgaatcgtt cataccacgc catcaacgta gtatcgtaat cagcaccgaa
    12301 gttatgccag tcttccatca caaagtgggg ttcgctggac tgagcaatct ggcgtacaga
    12361 gggcaggcaa ccgttcggga aaatatattt attaatccag ggatcaacat tcagatcggt
    12421 ttttttcgaa ccgatagtat ggagcaggaa tatgccttcc ggtttcaaat tacgatccac
    12481 caccgtaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc ccacagaaac
    12541 aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg tgacatccag
    12601 gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga cgcccaccac
    12661 gcttacgtca taattagatg ccatgtagtg tgccagtccg ccccagccgc agccaatatc
    12721 cagtacgcgc atcccaggtt ttaactgcaa tttttcacaa atcattttga gcttcgcctg
    12781 ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt gcatgaaggg
    12841 atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc cgactatcca
    12901 ggcacgtttt ttactctgca gattgaagag acgggcgccg gcaatacgca gcgtgtcttt
    12961 gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa aaaacatatc
    13021 cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca aagagccttc
    13081 ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg gggcagaacc
    13141 gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc agttgtcatc
    13201 cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac tggtcggtga
    13261 tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc gcagaaaaaa
    13321 ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc cgaaatgacc
    13381 atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta tcgtagcttt
    13441 aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt ttgttagcag
    13501 cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac ttcggcattg
    13561 ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag cgcgaccagc
    13621 actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat actgatcagc
    13681 caggaaacta ccagacttgc gaggaagcac agacccagtt gcagagtgtt ctgcaacgct
    13741 gcggcgcgac cagttgcgtg tgggaaggga cgcagagcct gggcgacaac aatagggtag
    13801 atcgcgccat tagcaatcgc catcacacag aatgggatca ggatttcgac cagcgacaca
    13861 tggctaatga agcccgcagc ccaggtcgca atgacgctga cagcaaacag caccagcaac
    13921 cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc ataaccacca
    13981 atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg gctgtagccc
    14041 atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc tgaacaggcc
    14101 gcgtatatca gcacattgcc gcgataggtt ttagaacgta gcaggtcggt aaaggtcaga
    14161 ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat aggcagaatc
    14221 agcaccacgg taatggcaaa aagggtggcg aaaatcgcct gccaggaaaa atggaccagc
    14281 agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg catgatggtc
    14341 gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc ttgccagata
    14401 accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag catagcggcg
    14461 ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc gattaataat
    14521 accggtttac gaccataacg atcggagagc ggcccccaca gaagctgggc tgcggcaaaa
    14581 ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg caggtcggcc
    14641 tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag tacgctcaaa
    14701 cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta aatctctcaa
    14761 ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt aatatttgat
    14821 gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact cgaagttgtt
    14881 gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca tcgcgttcct
    14941 tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc gctgtttgaa
    15001 cgacggcacc gtgatgtgga gctgaccgct gccggagcgt ggtttctcaa agaagggcgc
    15061 tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc gaacggctgg
    15121 cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac acggcagatg
    15181 atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca ggaagtgttc
    15241 aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg cgcaacacgc
    15301 gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag ctggagttgc
    15361 gttgttgcca gccatcaccc gctggcgttg atggatggcc cgttcagcga tgatacgttg
    15421 cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa gcgtattacc
    15481 tggttgctgg ataaccaaaa aagagttgtc gtgcctgact gggaatcatc ggcaacctgt
    15541 atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc gtggcttaat
    15601 gaggggaagt gggtagcatt agaactggaa aacccctttc cggattcggc atgttgtctg
    15661 acatggcagc aaaatgatat gtcgcctgcg ttaacctggt tgcttgagta tctcggcgat
    15721 agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc aacgggtgat
    15781 taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat caagcgcgga
    15841 tgcacttcaa tggactgcgg ttcttcacgt ttgttgacta tacgatccaa caacatgttg
    15901 aacgctgttt cacccaacga atcttttggc tgatggattg ttgtcagcgc cggcgtaaaa
    15961 tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg gacgcgcagc
    16021 cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc acagaagacg
    16081 gcagtagggc gatgcggctg cgacaggatt tgctgcatgg cgcgataacc ggattcaggt
    16141 tcaaagtcac cctgcacaat ccagctttcc ggcaccttga tcattgcttc ttccatcgcc
    16201 ttcataaaac cggcaaggcg gcctgcgcca gtgttacgtt ccagcgggcc ggggataacg
    16261 ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc gccttcgaac
    16321 gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc catcaccacc
    16381 attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta ctcagaacac
    16441 atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc ccgctgtttc
    16501 tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa gcaatttttt
    16561 tcaactgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc tagcaaaccg
    16621 atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga gtagtgtaat
    16681 tctttgatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg tgttttgttg
    16741 atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc ttttattgtt
    16801 gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa ggtaaacgtt
    16861 tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa acgacacgga
    16921 gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat tttggccgat
    16981 cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg agattttttc
    17041 atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa aggagaaaac
    17101 ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct cggtttgatc
    17161 gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc gcgttctccc
    17221 tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt ttgttatttt
    17281 catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg taatgacgcg
    17341 aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc gattggcgcg
    17401 tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat tgcgcgcggt
    17461 gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt tggcgtaatg
    17521 gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag tgcgttgatt
    17581 ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc ggattatatg
    17641 accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt tggtttgggt
    17701 tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt tgccactatg
    17761 tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg gttgggtgaa
    17821 accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat ttcaatggta
    17881 agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt gaaaaaagag
    17941 ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt actgggagct
    18001 ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat tacccacgca
    18061 acaccggtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc tatcggtaac
    18121 tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg ctttttgttg
    18181 ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaataagtt cggcgcagct
    18241 attagcatgg cggtgtgggg cgctgcaacc tttgcggtcg taccgccgtt acagatgcgc
    18301 gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat tggtgccttt
    18361 aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc tgggctggga
    18421 tacagctttg tgccggtgat gggggcgatt gtcgcgggac tggcattatt gctggtgttt
    18481 atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt aaacaaaaac
    18541 gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc ttgccgaatg
    18601 cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc aatactgcat
    18661 ttgccatcag ttattatgca gcgagatttt tcgctacgaa ttcccagttc accagcgccc
    18721 agaagtgctc aagatagcca ggacgtgcat tgcgatagtc gatgtaatat gcgtgttccc
    18781 agacatcaac ggttagcagc ggagtcgcat cggtggtcag cggagtaccc gcgttagagg
    18841 ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag ccagaaccaa
    18901 agtttttgat cgctgcatca gtaaactgag ctttgaaatc ggcaaagctg ccaaaagatg
    18961 cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt gccaggcagt
    19021 tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca ccttcagagc
    19081 tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttaatc aggttgttca
    19141 ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatagtt tccgcagaaa
    19201 tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat gacattgcta
    19261 ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat ggatagggta
    19321 gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa cagggtaaag
    19381 ttttagcgaa gtgtttttgg ggtcattacc cggcgagcgc caacatagtg gcgctgccag
    19441 tagtcttcac tgagagaagt gatttgaatt tcctgacctg tgcgcggtga ctgaataaat
    19501 ttgccgttgc cgacatacac gccgacatga tcggctgtac cgcgtccctg agtacggaaa
    19561 aagaccaggt cgccgttttt cagttcacta cgttcgattg gcgctgcatc gcgcaggtga
    19621 tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata agcgtaataa
    19681 accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg atatggctta
    19741 ccaatttgct gcatcagttt attcattgcc acttttgtgg ctttttgcac cttcgctttg
    19801 tgtgcgtcgg caagggttcc tgacgctgca tttttgactt tcacacaatg cgatttatag
    19861 cccttacgtt tggtacactt ttcggtaagg ctggctgagg ccgttttatt gacggtatgt
    19921 ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac ggaagaagcg
    19981 gttttgcttt tggtcgtact tttactggcg gcttttttcg ctgttttctg ggtctttttg
    20041 gtggttgccg tgcttttctt tttatctgct tttttggtga tatgagtggt agcagaactc
    20101 tccctggctt gctttgaagc atgggccata ggcgttaaag gcagggtggt aaaaagtaaa
    20161 gcacagagcg tgatcgaaat acggtttatc cgcgccactg agcgtccctc tgttaaggca
    20221 ggtctatcat gcctgcgtct gatttacaaa acttgatgat tctaatgcat agaagaagag
    20281 aaagttaata gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag
    20341 cagtgaaagc taaaaatggt cataaatacg ccagtttgcc gtgcatcagg gaagaaacga
    20401 atatttgaaa tgcaacaaat agagcaaagg gataaaatag caaaagcgtg atgaaaatgt
    20461 tattttccga tgccgcactg acccgctaca atgtcagatt actgccgtaa agaagtttga
    20521 ggaagcaaga caatgagcac cactatcgaa aaaatccaac gccagattgc tgaaaacccg
    20581 atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca
    20641 gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg ttgatattct gcagaatccg
    20701 gacattcgtg cggagctgcc gaaatatgct aactggccga ccttcccgca actgtgggtt
    20761 gacggcgagc tggtaggcgg ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg
    20821 cagcagctga tcaaagaaac tgccgctaaa tacaagtctg aagagccgga cgcggaataa
    20881 tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc agtcctttct ctatccccaa
    20941 tccagccctt gtggcgaact tgaaaatccc gcttcacgca gcagcgtaaa catcggcgtt
    21001 tgccagaccg gtagatcatt cacttctgtt agcgtaaagc gcagccgtgg ttcgcgacgc
    21061 agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta attcctcttt ttcctgccag
    21121 accagcattt ttttgccacc ttgcgccaga tagagtaaca atttgccgcc agaaacgacc
    21181 accagcgcac ccgcccgacg cgttggtacc agcgttgccg gatgtgcagg ccagggcaga
    21241 agatttcccc acacattagc cggatcgttg gcggagagcg ccactggtgt atagtggcgc
    21301 gtttgcgcgg cttgtgtcgc caggtcgcgc aagcggtcaa tagtcagacg ttcagcgaat
    21361 tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga atcttccata
    21421 cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga tattttcggc tatcaccgcc
    21481 tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgccag catcctttcg
    21541 gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt tggtgtgttg
    21601 taagataccc gcggcgagac gggttgtgca tagacaggac gcccacgacg agcccggtga
    21661 ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg taacggtgcc
    21721 caaatatcgc tggtgatgac gccttgccag accagcgccc ataacgcctc ttgcagtgcc
    21781 gagggatcaa ccgattcgcc gattttgtcg cgtatccgtt ggctgatttg ttgtgcaaac
    21841 caggctcctc cgtcagccag aacagcgact atcgcttgtt gcagcgccga acgattcgcc
    21901 tgatccgctt cggcgggagt gaacgattct gcagcatatt cctgtagatg cagtgccacc
    21961 aggccgtcat cttcacccag ttttttttgc cccgaccaga taaccgcacc ggttgccagt
    22021 aattcatcga gcacttctgg cgaatagtcg tgtacgcggg caggcaagat ctggctttcc
    22081 cagagtgagg cgggtaagcc gactccggca agctgttcga tcacccgcat cacgccatcc
    22141 accccctcat aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc atcggtggcg
    22201 ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc aacgggacgc
    22261 gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc
    22321 acccagatgt cttgttgcag attcatcacc agaccctgtt cgcgcagttg ctgaagctgc
    22381 tcttcgacaa tggcaatacc gagactaaac tcatgagcca gttgttcagc cgtgaccaga
    22441 gcatgagccc ggagatagcg tagaaagagg tcgcgaagcg ggtaactgac tctatgcaaa
    22501 taaatctctg gcagtgactc tggtaatcgt acgccgaggg catcgcgcag cctggcggca
    22561 tcatccatac aggcaagacg ctcctgtcca ctaatcatcg tcgggaagat gcgttttact
    22621 gtaagaagat tttccagata cgaggcaatc tcttcactgc tgcctgtatg ccgttgcgcc
    22681 aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct
    22741 ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctagcg aatgacctgc
    22801 gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc
    22861 agcgacagta cggatgcccg gcgttctgcc agcggggcgt cgctctggta cataaattcc
    22921 gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtagt cgttacatcg
    22981 gatatttgaa tttcgcctcc gttcaggcga cgcatcaaac gttccagtgc gggaagatca
    23041 taaacatctt gcagacattc gcgtagggtt tcgagaatgg ccggaaaatc cggatatccc
    23101 tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg ggtgcgatgg
    23161 cccggcgtgc ggcccggcat taataatgcc cgcgcggcgc attcgcgaaa acgggcggcg
    23221 aaaagtgccg aactgcctac cgcctcgcgg acaatttgca gcaacttttc tggttcaaac
    23281 aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc aacaatgccg
    23341 tcatcactgg cgaccacaga cgcgtcagcg ccccaaagcg catgtattcg cccggcaatc
    23401 gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat aatacgccaa
    23461 tcgccaattt catcacggca ccgttcgagg accagatgac gactccccgg aacaataccc
    23521 gtcgcgttgc gctgctcgtc aatcaacccc tgaatattgg cgatcgtatt ttcttcagcc
    23581 agccacgggg gaatagtccc ggaaaagaac gctccatccg ccagcaaatg aagaaaatcg
    23641 ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca gaaggggagc
    23701 cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat ttgctggatc
    23761 cgccatgagg ttgcgccgag agtgataata tcgttcaccc gcgactcata taccatctcc
    23821 tcatccagtt cacccacccg tcgcgaaccg gcctgttctt ccccttcggg taataacacg
    23881 ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacag ctgagcgcca
    23941 ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg aaaagcagaa
    24001 aaatcgccag agggatagcg tccggaaagc atatccagcg tggcgtcaaa aacacgtctt
    24061 ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc tacctgtaat
    24121 gcatccatcg ccgcggcggc aacggtgtgc tgcgcaagga cgtcgagtgg attatgcgga
    24181 ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc ggaatcgact
    24241 aagtcacgcc tggttcgggg gaaaaaaagc cctttggaga tacctcctat ctggtgtccg
    24301 gcacgtccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac ctgaatcacc
    24361 agctccacag cgcccatatc aatccccagt tcgagactgg aagttgcgac cacgcagcgt
    24421 aactcacccg atttcagcgc ctgctcggtg attgctcgtt gttctttaga gacggagccg
    24481 tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac ggttagaggt tgcgccagag
    24541 gtcgactcga aatgggccgc atcagcggcg atagacgggg aacgctgtaa gcgtgcggcg
    24601 taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cccgcgaatt agtaaagaca
    24661 atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata tggccagatg
    24721 gagccttccc ggccggcatg gctgtcttcg ccagtgccgc tggcgaccga tgagacatca
    24781 tccatattgg cgactggtac gacaatccgt atctgcggat gacgcattgc gggcgggttg
    24841 actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc tgagcgcaca
    24901 gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga
    24961 cttaacgcca gatgcgcacc acgtttactt cccgctaccg cgtggacttc atcaataatt
    25021 accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat
    25081 tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgcg ttcctgtgca
    25141 ggcgtatcgc cagtacggat ccccacgcga agattgactt ccgtttcgcc gcgccgccgc
    25201 cgttcatcgg caataccctt caacgggatc tgcaagttgc gctgaacgtc ggtgcccagg
    25261 gcttttatcg gtgaaatata gaggatgcgt gaggttttac gtttatgcgc ctcgcgggta
    25321 tcttcgccgc cttcgcggaa gagtcgatcg agggcgtaga gaaatgctgc cagcgttttc
    25381 ccggagccag tcggtgcaat caccagcgca tgttcgcctc gcgccgccac atgccaggtt
    25441 tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt
    25501 gaaaacacgt ccggcagggg cgatgaaggg tttggattat ctgccattaa cgctattccc
    25561 cgtgaatatc atgcatgatg cgtggaatag cctcactata gatgcctgaa tcattgcatg
    25621 tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag cctcccagac
    25681 gtttccagcg gttgacgatt tcacaaaaca gcaccgcagt gcgttcggtg tcgtacagcg
    25741 cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc tttgacaata
    25801 cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga
    25861 acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg tgatcgaaat
    25921 tggcgttgtg cgccaccata atggcgcggt tacagccgct cgctttaata cctttacgta
    25981 caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt
    26041 tcgggtcaat gccgttgaaa gcaagggctt ctggttgcaa atttgcgccg acaaatggtt
    26101 cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc attttcaggg
    26161 tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccagct gtttcaacat
    26221 cgatcacaac aggataaaag ccacgaaaac ggtcgcacag accggtaagt tgagcgttat
    26281 cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca aatattactt
    26341 gtcgatgcag taaagatgcg ggcgcgatga gttcgcgccc ggcaggagat tagttgccca
    26401 gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc ggatcttcca
    26461 caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc acgttacccc
    26521 cgttttgacg gattttttcg cacgcttcag cggcattatc tacgctaagc gcgatgtgac
    26581 cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt tcaatcaccg
    26641 cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat tccgggtttt
    26701 cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg gagcgttgca
    26761 gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca aaatggctaa
    26821 cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa tgttggatta
    26881 caacgtcggg taatcagtgt agccttccgc accgccacca tagaaacttt cagcacgctg
    26941 tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg caatccagtc
    27001 acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag ctttttctac
    27061 tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa
    27121 cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca gataagcaat
    27181 gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat tcgggccgtt
    27241 atccgtgttc tggaaagtac cgattggcga aacgcgaatg ccaatgcgat cggcacccca
    27301 ttcttcaatc ccggcatcga ccacttccag taccaaacgt gcgcgatttt ccacgctgcc
    27361 gccgtactga tcggtacgat ggtttgaaga aggagaaagg aactgatgca gcaaataacc
    27421 atgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag caatagcctg
    27481 acggaaatca ttgacgatac ttggaatctc ttccagttca agcgcacgcg gcatggatgt
    27541 ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag
    27601 tgctgaaggc gctactggtg cctggccgcc aggttgcagg ctggcgtgag aaatgcgtcc
    27661 ggtgtgccac agctgtacgg ccatatgccc attttcagca tgaacgccag cggtaatttt
    27721 tttccatgcg gcaatttgtt ccggactatg gatgccaggt gcaccagcat acccttttgc
    27781 ctgggcagaa atttgcgtgg cttcgctaat aatcaaaccg gcactggcac gttggcgata
    27841 gtattccgcc atcaacgggg tagggatgtc acccggttca atactgcgca gacgcgtcag
    27901 cggtgccata aaaatacggt ttgccgcagt tattgcgccc actttcagtg gggaatacag
    27961 tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct gctaaacggc
    28021 aggcgtcgca ataatggttt ttacatgtgc cagcgcgttt tccagtggct cggaattgcg
    28081 cgaaattttg gcctgcagat tcgcacccag ccatagtgcg taaagcactt gtgcctgttg
    28141 cagcggttcg ccacaaaagg ttaaacaatg gctatcacgg ccattttcca gagcctgcga
    28201 gagcagggcg atcacgccgc gagcgccttt atccatcgcg ctacgcatat cttctgacag
    28261 atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc catgttggca
    28321 aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc
    28381 ggattgcagc aactcagtca gtcgttggtg atatgtggcg taatggcgct caagcatggc
    28441 aacgccaaac gcttctttag agcgaaagta gtgatagaag gaccctttcg gcacttcagc
    28501 ggtttttagt aattcgctta gccccatccc ggtgaatcca cgttgcaggc aaagttgctc
    28561 gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca tgcgttggag
    28621 tgtagtagac cagtcggtct agtgcaagca aagttgctac tttgggcatt aacagcttca
    28681 ataatggtaa taatcttatt tgaggagggc gaagtggcgg agcaattaga gttctttccc
    28741 gtccagagcc cgtgccgggg aatttgccag tctgacgaac gcgggttttg tcgcggctgt
    28801 tttcgaagcc gggatgagcg ttttaactgg aataaaatga gcgatggcga aaaacaagag
    28861 gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac gtgcaaataa gccagcgtca
    28921 tcagacgaac cggagcaacc atcactcttt tgagcctaat cttgcgtata ctcctctcat
    28981 atctgataag aggaagcgat tatggttcag cgtattacta ttgcgccgca aggcccggag
    29041 ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaacatgtc cgcccgccag
    29101 ctggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga ccatgctgat
    29161 atttatggtg gctatcagtg cgaagcggcg tttggcgagg cgctgaaact ggcacctcac
    29221 ctgcgtgagc ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc gcgtgaagaa
    29281 aacgtcattg gtcattacat cactgaccgc gatcacatca ttaagagcgc cgaacagtcg
    29341 ctaattaatc ttgcgaccga tcatctggat ctgctgttaa tccaccgccc ggacccgtta
    29401 atggatgccg atgaagtggc ggacgcgttc aaacatctgc atcagagcgg caaagtgcgt
    29461 cattttggcg tatccaactt cacgcctgcg caatttgccc tgttgcaatc acgtctgccg
    29521 tttacccttg ccactaatca ggtggaaata tccccggtac atcagccgtt actgctggat
    29581 ggcacgctcg accaactgca acaactgcgt gttcgtccga tggcgtggtc ctgccttggt
    29641 ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact ggctgtggtg
    29701 gcggaggagt taaacgcggg ctcgattgaa caggtggttt acgcctgggt attacgttta
    29761 ccgtcgcagc cgctgccaat tatcggctga ggtaaaattg agcgcgtacg ggcagctgtc
    29821 gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa agcggcactg
    29881 gggtacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg tgtcactggt
    29941 ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt agtctggcca
    30001 ttctggcgct ggttgttgcg accggcgcac aagctgccag tgaaaaagtc gagatgaacc
    30061 tcgtcacgtc gcaaggggtt gggcagtcaa ttggtagcgt caccattact gaaaccgata
    30121 aaggtctgga gttttcgccc gatctgaaag cattacctcc cggtgaacat ggcttccata
    30181 ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc gccgcggaat
    30241 ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg
    30301 ggcatttagg cgatctgcct gcactggtcg tcaataatga cggcaaagct accgatgccg
    30361 tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg atggtccacg
    30421 ttggcggcga taatatgtcc gatcaaccta aaccgctggg cggtggcggt gaacgctatg
    30481 cctgtggtgt aattaagtaa ttaagaggcc agcgtacctt gcggtggtgc ttgctcaagt
    30541 tgcgaaagcg agcagtacag ccgccagaca attgccgcca gctcacgggc ggcaggttga
    30601 tgatggcggg caaggctgtc gcaaatccgc tgtaattctt caagtgtggc cgccagtgat
    30661 ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca gttatcccgt
    30721 actcgcgata acggatcgga acgcgattcc cagtcgcgta attgccagac aacatgagaa
    30781 cagttcagca gcactatacc ccagcgtaat aaccaacggc gagccagcgc atcctggctg
    30841 ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc actcagtgtt
    30901 ggatggcggc ttagttgatc gacaaaatcc cggcgcagcg cgcgaatatg gcggcggctt
    30961 ttgcgagcat ccgatcctgg acgcagaatg gcgaacgcta accacgccaa cgcgacgcca
    31021 acgatttttg ccagattatc gttaagaaaa tcagcaaaat tatacaccgg gggattagtg
    31081 acggcgataa aagaacccat aaaaacaatc agttgccccc acaatgcggc aaatttgggc
    31141 atctgcaatt taagaagctg cattgtcgcc agcagtggaa agagaaacag taaaaattgc
    31201 cacagatcgc taatctggac catcagacca aatttgacca caaagctgaa tagcgaaagt
    31261 aacaccagcg tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac ggcggagtag
    31321 agtacgcagc taatcgctgc cagcgttaat gcattggcac cggcatccca ttgcgaagca
    31381 atactccatg cgccaatcat catcaacgta caaaatgtac gcagtccgct ccacatagct
    31441 tcggcgttat cggtatgacg agccaggccg gatgtccgtg ggagtctggc atgatcatct
    31501 acatcgcttt gcagacgatg taattcctga ctactttgca gataaaggcg gcaaaaataa
    31561 cgtagtcgct gccagaaggc gacgtgccga tagtcggcga cgttggtcgg gcgtagcggg
    31621 gcgataatac gtgcgacggt gtaaacatct gtttgcgaac tggcgagcgc cgtcagcaac
    31681 tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa catacgtcgc
    31741 aggctggaga tgacactggt catacgtaat tgctggtgga gcagcgcatt aaggcgcgcg
    31801 ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa attcatggtc
    31861 agtatctgcc caatcacccc ttcatgtgct gcacgaatgg catcggttgt ttcaggctgc
    31921 cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc ggttaaaagg
    31981 gctgtagcat cggaactgct aggcaggatc atcatcatca tgccgccgca caaaataccg
    32041 acaatcacct cgcaaacgcg cgcctgagcg atatcccaca gctggctggc ctcagtaata
    32101 ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa tgcatacgcg
    32161 acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc cgacatgctc
    32221 aatagaaaaa accacggctc attgagcgta tgcccggcaa gaagcagtgc cgcaatggct
    32281 cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc aacggtggga
    32341 aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt caaataatag
    32401 gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg ccattgcgcc
    32461 agcgtggccc tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt cattattgac
    32521 ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga actttatcca
    32581 gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca ggggacgtta ggtttcacat
    32641 ccggaataag gcttgagtca ctttcaacgc tctgatcata aatcgcccga ccgatactgg
    32701 aaacgtgacc ctgtaacgtt ttgttatcac tatagagggt aatttgtgct ggtgcacctt
    32761 cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac gagtggctgt
    32821 cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg gtagtgagat
    32881 tagtcaccca tccactcacc ggggcgcgaa tttccgtttg cgccagttgc cattgtgtct
    32941 gtttcagcgt cgcctgtgcg gcatcaacac tggcctgcat cgctttaaca ttgaggttag
    33001 cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga cgattcgctt
    33061 cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt gcattcagct
    33121 cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg ttaaccagct
    33181 ggttgtcttt tatattcagc tcaacaatac gtcccgacac ctgtggggtg atagaaacct
    33241 gttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa ttccacagcc
    33301 accatccggc aagaaccgca actaccgcta caatgattgt tgagaaatac ttaattgttt
    33361 taattgacat agttaccacg caatcagtat tgccagagca aggcaaacgc aaatcgcaaa
    33421 cagcgataaa tccattaaca agggatgcca gatgtcaccg gcgtagatcc agccacgaag
    33481 caggcggtgt acgacaagcc agatgacgaa tccaaacgcg aaggctttga aaaacggagg
    33541 aaagtagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg cattgagcat
    33601 aaacttcaca aagtagattc ctttacgacc gaatatattg cgttagatta ataaatattc
    33661 tttaactgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc
    33721 agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc cgcataatat
    33781 cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac
    33841 ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac
    33901 aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag tcgcaaattc
    33961 aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg
    34021 aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta
    34081 aactgacgga aaaggcagag ccgctgatca gcgaaatgga agctgttatt aacaaaaccc
    34141 gcgcggaaat tttacatggc atctccgcag aggaactgga acaactgatt acgctcatcg
    34201 caaaactaga gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt
    34261 ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc
    34321 attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac
    34381 caccatgatg gtattaccat cgtctttacg aatttccagc tcgacacctt gcgttttgtt
    34441 cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc
    34501 cagagaacgc ccggttccgc caccaacagt atttcccagg aaaccaccaa gaacagcacc
    34561 gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt
    34621 aacgatggtt ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc
    34681 ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac
    34741 caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc
    34801 ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag
    34861 caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga
    34921 tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga
    34981 aaagtttatg tttgagtgtg cgcaaacgtc agattgaaaa aggatgaact atgaaatcgg
    35041 gccgctttat tggcgttatg tcaggcacca gccttgatgg tgttgatgtt gtgttggcga
    35101 caattgatga acaccgggtc gcacagctgg caagtttgag ctggccgatc ccggtgtctc
    35161 tgaaacaggc tgtactggat atttgccagg gccagcagct tacactttcg cagtttggac
    35221 agcttgatac tcaactcggg cgactttttg ctgatgcggt caaggccttg cttaaggaac
    35281 aaaacctgca ggcgagagat atagttgcga tcggctgtca cggtcaaacc gtctggcatg
    35341 aaccgacggg cgtggcacca cacactttac agattggcga taacaatcaa attgtggcac
    35401 gcaccggaat tacggttgtc ggtgattttc gccgtcgcga tattgccttg ggaggacaag
    35461 gcgcaccgct ggtacctgcg ttccatcatg ccttgctggc tcacccaacc gagcgacgaa
    35521 tggtgctcaa tattggcggc atcgccaatc tgtcactgct cattcctggg cagccggttg
    35581 gcggctacga taccggtcct ggtaacatgc tgatggatgc ctggatctgg cgtcaggccg
    35641 gtaaacctta cgataaagat gccgagtggg cacgggcggg taaagttatt ctcccactgc
    35701 tgcaaaatat gctcagcgac ccatatttct cgcaacctgc accgaaaagc accggacgcg
    35761 aatactttaa ctacggctgg ctggagcgcc atttacgcca ttttccgggt gttgatcccc
    35821 gtgatgttca ggccacactg gcagaactca ccgccgtgac catttctgaa caagttttgt
    35881 tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat ccgctactca
    35941 tggcgcgtct ggcggcatta ctgccaggca cagaagtcac caccaccgat gccgttggca
    36001 ttagtggcga tgacatggaa gcattggctt tcgcttggct tgcctggcgg acgctggcgg
    36061 gattaccagg aaatctgcct tccgtcactg gcgcaagcca ggagacggtt ctgggggcta
    36121 ttttccccgc caactcgtga tatcagaatt aactgaattt tttcttatcg tgtctgccgt
    36181 tacacttgga agattaagga ggatatttgc cctccggacc aggaaagtct tcgggatatg
    36241 actatgaaaa aactgttaat tatcattttg ccggtccttc tctccggctg tagcgccttt
    36301 aatcaactgg ttgagcgaat gcaaaccgat acgctggaat accagtgtga tgaaaaaccg
    36361 ttgacggtca aactgaataa tccgcgccag gaagtcagtt ttgtttacga taatcaacta
    36421 ctgcatctca aacagggcct ttcagcctct ggcgcgcgtt actccgacgg gatctatgtt
    36481 ttctggtcga aaggtgagga agcgactgtc tataaacgcg accgcatcgt cttgaataac
    36541 tgtcagttac aaaatccaca gcgttgagat ttttccaggg gcggcgcaca atagcgtcac
    36601 ccacagacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc aaatcgcgca
    36661 tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatcttcccg ccgatccatt
    36721 aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg accctaccgc
    36781 gatggtggtc gctaccgtgg atgaacatgg tcagccttat cagcgcatcg ttttactcaa
    36841 acattacgac gaaaaaggca tggtgtttta caccaacctc ggtagccgta aagcacatca
    36901 aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg aacgccaggt
    36961 gatggtgatc ggtaaagcag aacgactttc gactctcgaa gtgatgaaat attttcatag
    37021 ccgcccgcgt gatagccaga ttggttcatg ggtttcgaag cagtccagtc gcatttctgc
    37081 ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac aaggcgaagt
    37141 gccattgccg agcttttggg gcggttttcg cgtcagcctt gaacagattg agttctggca
    37201 gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg atgcgtggaa
    37261 gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc tggtactcct
    37321 gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa
    37381 ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat taaacaattg
    37441 caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga gcgactggcg
    37501 caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt gcatttgggg
    37561 catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg
    37621 ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag
    37681 ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa tccgcaagca ggttgccccg
    37741 ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta tgactggttc
    37801 ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc cgttaaccag
    37861 atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg gatttcgttc
    37921 actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa caaacagtac
    37981 ggtgtggtgc tgcaaattgg tggttctgat cagtggggta acatcacttc tggtatcgac
    38041 ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcaccaaa
    38101 gcagatggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa
    38161 accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc
    38221 ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctgga agaagaagat
    38281 aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gactcgtctg
    38341 gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct gttcagcggt
    38401 tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg
    38461 gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga actgcaacct
    38521 tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa
    38581 cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta
    38641 ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat taagtggaaa
    38701 gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga aaaatattct
    38761 cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag agtttccgat
    38821 gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta atcacaccca
    38881 atacggcaaa tggactggct gcgtgatgcc gcccagccat ttaaccgaaa ttgtgcaagg
    38941 cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc
    39001 ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag cgaatccgca
    39061 ggcgaaatat ttttgcgatc cggtaatggg tcatccagaa aaaggctgta tcgttgcacc
    39121 gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca ttgcgccgaa
    39181 tcttgttgag ctggaaatac tctgtgagca tccggtaaat aacgtcgaag aagcggttct
    39241 ggcagcgcgc gaactcattg cgcaagggcc acaaattgta ttggttaaac acctggcgcg
    39301 agctggctac agccgtgacc gttttgaaat gttgctggtc accgccgatg aagcctggca
    39361 tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac
    39421 gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca
    39481 tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagtt
    39541 gcaagtggtt gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa
    39601 gctctgaaat atctcatttc ggcccacaac gaactgtggg ccgacggcta ttactttaag
    39661 ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc
    39721 gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagtaccgta
    39781 aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt
    39841 gcctcgttca catattgcag tttcttctcc agctgcgcgc gaactgtcgg tttgtactct
    39901 tccggtgtat cagggcgaaa cagaggtgtg aaacctttat gcagctcggt ggcgatgtaa
    39961 ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg
    40021 tcggggacgc tgtcggcaag atactgcata atcgctacac cttccgtcag caaagtaccg
    40081 tcatccagca gcaatgcagg tacctgtccc ttagggttaa cggcaaagta atcgtcaccg
    40141 ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca
    40201 cgcagggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt
    40261 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaagccgc
    40321 taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac ggctgctttc
    40381 gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttcggcgc ggtcagcagc
    40441 atcagtactg caataacggc agtagcgaca ccaatctgca agaatacgcg accatagact
    40501 tccagtgaca tcagcggatc ggtaacgtta tccggcacag ccatcatacc cgcaacataa
    40561 ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat gaagcccatc
    40621 agacgctgcg gaacgagttg tgcaaccatt gccagaccca gaccagagat catcagttcc
    40681 ccgatactct gcaggccata gctcgcgacc agccagctta cagacacgat acctgcgtca
    40741 gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat caccataccg
    40801 attgcaaact tggttggcat cggcagggta tcgcccatct tgttatagat agcggccaga
    40861 atcgggctac caatgatgat ccagaacggg ttcagtgcct gatactgctc aggttctacg
    40921 gccaggccca gaatggtatg ttcaacgtta cgaatcgcaa agaagttcag tgacgttggc
    40981 atctggctgt acagcacgaa gaagataatg gcttcgagca tcaggatgaa ggcaacgatc
    41041 attttacgac gcgcagcacc tttcatcgcg aaggcttctt taccgaagat aaccacgata
    41101 ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag cagccaggtg
    41161 gcgatagcga tcagtgccac aacaccaata atggtcagca gcaggttacg gtagttgata
    41221 ggctcgaagt ctggttttga accgtactgt ttaacccagc gttggcagaa ggcgaagtta
    41281 acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca gccgtatttc
    41341 gcggccagcc acggggtggc gatcatagag aagaaagagc cgatgttgac ggacatgtag
    41401 tacatggtga atgcaccgtc cagacgcggg tcgtttttct catagcatgt agaaagcaga
    41461 gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat acccatataa
    41521 acgataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc cagcacaata
    41581 gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca gccgccgata
    41641 gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga atccgcttca
    41701 gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc gtaataacca
    41761 aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg tttgaaagcg
    41821 ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac ctcttttttt
    41881 acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg
    41941 ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct
    42001 ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat
    42061 ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga
    42121 tgatggcttg acggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt
    42181 ttcttaaaaa tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta
    42241 aatgatttta acctgatt
//
LOCUS       DAZFKY010000018        41931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFKY010000018 DAZFKY010000000
VERSION     DAZFKY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            294..380
                     /gene="leuL"
                     /locus_tag="ACHKCL_000899"
     CDS             294..380
                     /gene="leuL"
                     /locus_tag="ACHKCL_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5761922.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            473..2044
                     /gene="leuA"
                     /locus_tag="ACHKCL_000900"
     CDS             473..2044
                     /gene="leuA"
                     /locus_tag="ACHKCL_000900"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5761923.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2044..3135
                     /gene="leuB"
                     /locus_tag="ACHKCL_000901"
     CDS             2044..3135
                     /gene="leuB"
                     /locus_tag="ACHKCL_000901"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5761924.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3138..4538
                     /gene="leuC"
                     /locus_tag="ACHKCL_000902"
     CDS             3138..4538
                     /gene="leuC"
                     /locus_tag="ACHKCL_000902"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5761925.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4549..5154
                     /gene="leuD"
                     /locus_tag="ACHKCL_000903"
     CDS             4549..5154
                     /gene="leuD"
                     /locus_tag="ACHKCL_000903"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5761926.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5262..5393)
                     /gene="sgrT"
                     /locus_tag="ACHKCL_000904"
     CDS             complement(5262..5393)
                     /gene="sgrT"
                     /locus_tag="ACHKCL_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5761927.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5482..7140
                     /gene="sgrR"
                     /locus_tag="ACHKCL_000905"
     CDS             5482..7140
                     /gene="sgrR"
                     /locus_tag="ACHKCL_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5761928.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7369..7938)
                     /gene="espY2"
                     /locus_tag="ACHKCL_000906"
     CDS             complement(7369..7938)
                     /gene="espY2"
                     /locus_tag="ACHKCL_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO5761929.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8180..8279
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8310..9293
                     /gene="thiB"
                     /locus_tag="ACHKCL_000907"
     CDS             8310..9293
                     /gene="thiB"
                     /locus_tag="ACHKCL_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5761930.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9269..10879
                     /gene="thiP"
                     /locus_tag="ACHKCL_000908"
     CDS             9269..10879
                     /gene="thiP"
                     /locus_tag="ACHKCL_000908"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5761931.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10863..11561
                     /gene="thiQ"
                     /locus_tag="ACHKCL_000909"
     CDS             10863..11561
                     /gene="thiQ"
                     /locus_tag="ACHKCL_000909"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5761932.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11648..12412)
                     /gene="yabI"
                     /locus_tag="ACHKCL_000910"
     CDS             complement(11648..12412)
                     /gene="yabI"
                     /locus_tag="ACHKCL_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5761933.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12498..13376)
                     /gene="araC"
                     /locus_tag="ACHKCL_000911"
     CDS             complement(12498..13376)
                     /gene="araC"
                     /locus_tag="ACHKCL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5761934.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13715..15415
                     /gene="araB"
                     /locus_tag="ACHKCL_000912"
     CDS             13715..15415
                     /gene="araB"
                     /locus_tag="ACHKCL_000912"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5761935.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15426..16928
                     /gene="araA"
                     /locus_tag="ACHKCL_000913"
     CDS             15426..16928
                     /gene="araA"
                     /locus_tag="ACHKCL_000913"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5761936.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17128..17823
                     /gene="araD"
                     /locus_tag="ACHKCL_000914"
     CDS             17128..17823
                     /gene="araD"
                     /locus_tag="ACHKCL_000914"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5761937.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17898..20249
                     /gene="polB"
                     /locus_tag="ACHKCL_000915"
     CDS             17898..20249
                     /gene="polB"
                     /locus_tag="ACHKCL_000915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5761938.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20413..23319
                     /gene="rapA"
                     /locus_tag="ACHKCL_000916"
     CDS             20413..23319
                     /gene="rapA"
                     /locus_tag="ACHKCL_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5761939.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23331..23990
                     /gene="rluA"
                     /locus_tag="ACHKCL_000917"
     CDS             23331..23990
                     /gene="rluA"
                     /locus_tag="ACHKCL_000917"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5761940.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24203..24381
                     /locus_tag="ACHKCL_000918"
                     /pseudo
     CDS             24203..24381
                     /locus_tag="ACHKCL_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24408..25160)
                     /gene="espY1"
                     /locus_tag="ACHKCL_000919"
     CDS             complement(24408..25160)
                     /gene="espY1"
                     /locus_tag="ACHKCL_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO5761941.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATVDGATVDGATHLYDEVI
                     IINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(25455..26270)
                     /gene="djlA"
                     /locus_tag="ACHKCL_000920"
     CDS             complement(25455..26270)
                     /gene="djlA"
                     /locus_tag="ACHKCL_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5761942.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26525..28879
                     /gene="lptD"
                     /locus_tag="ACHKCL_000921"
     CDS             26525..28879
                     /gene="lptD"
                     /locus_tag="ACHKCL_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5761943.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28932..30218
                     /gene="surA"
                     /locus_tag="ACHKCL_000922"
     CDS             28932..30218
                     /gene="surA"
                     /locus_tag="ACHKCL_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5761944.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30218..31207
                     /gene="pdxA"
                     /locus_tag="ACHKCL_000923"
     CDS             30218..31207
                     /gene="pdxA"
                     /locus_tag="ACHKCL_000923"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5761945.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31204..32025
                     /gene="rsmA"
                     /locus_tag="ACHKCL_000924"
     CDS             31204..32025
                     /gene="rsmA"
                     /locus_tag="ACHKCL_000924"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5761946.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            32028..32405
                     /gene="apaG"
                     /locus_tag="ACHKCL_000925"
     CDS             32028..32405
                     /gene="apaG"
                     /locus_tag="ACHKCL_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5761947.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32412..33260
                     /gene="apaH"
                     /locus_tag="ACHKCL_000926"
     CDS             32412..33260
                     /gene="apaH"
                     /locus_tag="ACHKCL_000926"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5761948.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33257..33571)
                     /locus_tag="ACHKCL_000927"
     CDS             complement(33257..33571)
                     /locus_tag="ACHKCL_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO5761949.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33574..33807)
                     /locus_tag="ACHKCL_000928"
     CDS             complement(33574..33807)
                     /locus_tag="ACHKCL_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5761950.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33893..34372)
                     /gene="folA"
                     /locus_tag="ACHKCL_000929"
     CDS             complement(33893..34372)
                     /gene="folA"
                     /locus_tag="ACHKCL_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5761951.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34564..36426)
                     /gene="kefC"
                     /locus_tag="ACHKCL_000930"
     CDS             complement(34564..36426)
                     /gene="kefC"
                     /locus_tag="ACHKCL_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5761952.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36419..36949)
                     /gene="kefF"
                     /locus_tag="ACHKCL_000931"
     CDS             complement(36419..36949)
                     /gene="kefF"
                     /locus_tag="ACHKCL_000931"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5761953.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37057..38388)
                     /gene="yaaU"
                     /locus_tag="ACHKCL_000932"
     CDS             complement(37057..38388)
                     /gene="yaaU"
                     /locus_tag="ACHKCL_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5761954.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38446..38733)
                     /gene="fixX"
                     /locus_tag="ACHKCL_000933"
     CDS             complement(38446..38733)
                     /gene="fixX"
                     /locus_tag="ACHKCL_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5761955.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38730..40016)
                     /gene="fixC"
                     /locus_tag="ACHKCL_000934"
     CDS             complement(38730..40016)
                     /gene="fixC"
                     /locus_tag="ACHKCL_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5761956.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40067..41008)
                     /gene="fixB"
                     /locus_tag="ACHKCL_000935"
     CDS             complement(40067..41008)
                     /gene="fixB"
                     /locus_tag="ACHKCL_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5761957.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41023..41793)
                     /gene="fixA"
                     /locus_tag="ACHKCL_000936"
     CDS             complement(41023..41793)
                     /gene="fixA"
                     /locus_tag="ACHKCL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5761958.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10040 a        11437 c        10923 g         9531 t
ORIGIN      
        1 aaaacatata attcaataca aatcatcagg ataggttttg caacgcgtgc attttgtccc
       61 ctttttcctc gtggattaga tgcaaaaatt tattctgaaa tatgtcaacc gatgaaaagc
      121 atcggtagtt aagcagaaat taatatcgct tgttttaacc acagcagcac aattagctaa
      181 tcttacggat gcagaactca tgctggcggg acgtttttat tgcgtcaggg ttgacatccg
      241 tttttgtatc cagtaactct aaaagcatat cgcattcatc tggagctgat ttaatgactc
      301 acatcgttcg ctttatcggt ctactactac taaacgcatc ttctttgcgc ggtagacgag
      361 tgagcggcat ccagcattaa gccagcacgc agtcaaacaa aaaacccgcg ccattgcgcg
      421 ggttttttta tgcccgaagc gaggcgctct aaaagagaca aggacccaaa ccatgagcca
      481 gcaagtcatt attttcgata ccacattgcg cgacggtgaa caggcgttac aggcaagctt
      541 gagtgtgaaa gaaaaactgc aaattgcgct ggcccttgag cgtatgggtg ttgacgtgat
      601 ggaagtcggg ttccccgtct cttcgccggg tgattttgaa tcagtgcaaa ccatcgctcg
      661 ccaggttaaa aacagccgtg tatgcgcgtt agctcgctgc gtggagaaag atatcgacgt
      721 ggcggccgaa tccctgaaag tcgccgaagc cttccgtatt cataccttta ttgccacttc
      781 gccaatgcac atcgccacca agctgcgcag cacgctggac gaggtgatcg aacgcgctat
      841 ctatatggtg aaacgcgccc gtaattacac cgatgatgtt gaattttctt gcgaagatgc
      901 cgggcgtaca cccattgccg atctggcgcg agtggtcgaa gcggcgatta atgccggtgc
      961 caccaccatc aacattccgg acaccgtggg ctacaccatg ccgtttgagt tcgccggaat
     1021 cattagcggc ctgtatgaac gcgtgcctaa catcgacaaa gccattattt ccgtacatac
     1081 ccacgacgat ttgggcctgg cggtcggcaa ctcactggcg gcggtacatg ccggtgcacg
     1141 ccaggtggaa ggtgcaatga acgggatcgg cgagcgtgcc ggtaactgtt ccctggaaga
     1201 agtcatcatg gcgattaaag ttcgtaagga tattctcaac gtccacaccg ccattaatca
     1261 ccaggagata tggcgcacca gccagttagt tagccagatt tgtaatatgc cgatcccggc
     1321 aaacaaagcc attgttggca gcggcgcatt cgcacactcc tccggtatcc accaggatgg
     1381 cgtgctgaaa aaccgcgaaa actacgaaat catgacacca gaatctattg gtctgaacca
     1441 aatccagctg aatctgacct ctcgttcggg gcgtgcggcg gtgaaacatc gcatggatga
     1501 gatggggtat aaagaaagtg aatataattt agacaatttg tacgacgcct tcctcaagct
     1561 ggcggacaaa aaaggtcagg tgtttgatta cgatctggag gcgctggcct tcatcggtaa
     1621 gcagcaagaa gagccggagc atttccgtct ggattacttc agcgtgcagt ctggctctaa
     1681 cgatatcgcc accgccgccg tcaaactggc ctgcggcgaa gaagtcaaag cagaagccgc
     1741 caacggtaac ggtccggtcg atgccgtcta tcaggcgatt aaccgcatca ctgactataa
     1801 cgtcgaactg gtgaaataca gcctgaccgc caaaggtcac ggtaaagatg cgctgggtca
     1861 ggtggatatt gtcgccaact acaacggtcg ccgcttccac ggcgtcggcc tggccaccga
     1921 tattgtcgag tcctccgcca aagccatggt gcacgtactt aacaatatct ggcgtgccac
     1981 agaagtcgaa aaagagttgc aacgcaaagc tcaacacaac gaaaacaaca aggaaaccgt
     2041 gtgatgtcga agaattacca tattgccgta ttgccggggg atggcattgg tccggaagtg
     2101 atgacccagg cgctgaaagt gctggatgcc gtgcgcaacc gctttgcgat gcgcatcact
     2161 accagccatt acgatgtagg cggcgcagcc attgataacc acgggcaacc actgccgcct
     2221 gcgacggttg aaggttgtga gcaagccgat gccgtgctgt ttggctcggt aggcggcccg
     2281 aaatgggaac atttaccacc ggaccagcaa ccagaacgcg gcgcgctgtt gcctttgcgt
     2341 aagcacttca aattattcag caacctgcgt ccggcaaaac tgtatcaggg gctggaagca
     2401 ttctgtccgc tgcgtgcaga cattgccgca aacggcttcg acatcctgtg cgtgcgcgaa
     2461 ctgaccggcg gcatctattt cggtcagcca aaaggccgcg aaggtagcgg acaatatgaa
     2521 aaagcgtttg ataccgaggt gtatcaccgt tttgagatcg agcgtattgc ccgcatcgcg
     2581 tttgaatctg ctcgtaaacg tcgccataaa gtgacgtcga tcgataaagc caacgtgctg
     2641 caatcctcta ttttatggcg tgaaatcgtc aacgagatcg ccacggaata cccggatatc
     2701 gaactggcgc atatgtacat cgacaacgcc accatgcagc tgattaaaga tccatcacag
     2761 tttgacgttc tgctgtgctc caacctgttt ggcgacattc tgtctgacga gtgcgcaatg
     2821 atcactggct cgatggggat gttgccttcc gccagcctga acgagcaagg ttttggactg
     2881 tatgaaccgg cgggcggctc ggcaccagat atcgcaggta aaaacatcgc caacccgatt
     2941 gcgcaaattc tgtcgctggc actgctgctg cgttacagcc tggatgccga tgatgcggct
     3001 tccgccattg aacgcgccat taaccgcgca ttagaagaag gcattcgcac cggggattta
     3061 gcccgtggcg ctgccgccgt tagtaccgat gaaatgggcg atatcattgc ccgctatgtg
     3121 gcagaagggg tgtaatcatg gctaagacgt tatacgaaaa attattcgac gctcacgtag
     3181 tgtacgaagc cgaaaacgaa accccgctgt tatatatcga ccgccacctg gtgcatgaag
     3241 tgacctcacc gcaggcgttc gatggtctgc gcgcccacgg tcgcccggta cgtcagccgg
     3301 gcaaaacctt cgccaccatg gatcacaacg tctctaccca gactaaagac attaatgcct
     3361 gcggtgaaat ggcgcgcatc cagatgcagg agctgatcaa aaactgcaaa gaatttggcg
     3421 tcgaactgta tgacctgaat cacccctatc aagggatcgt ccacgtaatg gggccggaac
     3481 agggcgtgac cttgccgggg atgaccattg tctgcggcga ctcgcatacc gccacccacg
     3541 gcgcgtttgg cgcactggcc tttggtatcg gcacttccga agttgaacac gtactggcaa
     3601 cgcaaaccct gaaacagggc cgtgcgaaga ccatgaaaat tgaagtccag ggcaaagccg
     3661 cgccgggcat taccgcaaaa gatatcgtgt tggcaattat cggtaaaacc ggtagcgcag
     3721 gcggcaccgg gcatgtggtg gagttttgcg gcgaagcaat ccgtgattta agcatggaag
     3781 gtcggatgac cctgtgcaat atggcaatcg aaatgggcgc gaaagccggt ctggttgcac
     3841 ctgacgaaac cacctttaac tatgtcaaag gccgtctgca tgcgccgaaa ggcaaagatt
     3901 tcgacgacgc cgttgcctac tggaaaaccc tgcaaaccga cgaaggcgca actttcgata
     3961 ccgttgtcac tttgcaggca gaagagattt caccgcaggt cacctgggga actaacccag
     4021 gccaggtgat ttccgtgaac gacaatattc ccgatccggc ttcgtttgcc gatccggttg
     4081 aacgcgcgtc ggcagaaaaa gcgttggcct atatggggct gaaaccgggt attctgctga
     4141 ccgaagtggc tatcgacaaa gtgtttatcg gttcctgcac caactcacgt attgaagatt
     4201 tacgcgcggc ggcggaaatc gccaaagggc ggaaagtcgc gccaggcgta caggcgctgg
     4261 tggttcccgg ctctggtccg gtaaaagcgc aggcggaagc agaaggtctg gataaaatct
     4321 ttattgaagc cggttttgaa tggcgcttgc ctggctgctc aatgtgtctg gcgatgaaca
     4381 acgaccgtct gaatccgggc gaacgttgtg cctccaccag caaccgtaac tttgaaggcc
     4441 gtcaggggcg cggcgggcgc acgcatctgg tcagcccggc aatggctgcc gccgcggctg
     4501 tgaccggcca tttcgccgac attcgcaaca ttaaataagg agcacaccat ggcagagaaa
     4561 tttatcaaac acacaggcct ggtggttccg ctggatgccg ccaatgtcga taccgatgca
     4621 atcatcccga aacagttttt gcagaaagtg acccgtactg gttttggcgc gcatctgttt
     4681 aacgactggc gttttctgga tgaaaaaggc caacagccaa acccggactt cgtgctgaac
     4741 ttctcgcagt atcagggcgc ttccattttg ctggcacgag aaaacttcgg ctgtggctcc
     4801 tcgcgtgagc acgcgccctg ggcattgacc gactacggtt ttaaggtggt gattgcgccg
     4861 agttttgctg acatcttcta cggcaatagc tttaacaacc agctgctgcc ggtgaaatta
     4921 agcgacgcag aagtggatga gctgtttgcg ctggtgaaag ccaatccggg gattcatttt
     4981 gatgtagacc tggaagcgca agaggtgaaa gcgggtgaga aaacctatcg ctttaccatt
     5041 gatgccttcc gccgccactg catgatgaac ggtctggaca gcattgggct taccttgcag
     5101 cacgacgacg ccattgcggc ttatgaagcg aagcaacccg cgtttatgcg ttagtttgta
     5161 caccgtccga tactcaatcg ccaaaaagaa aaaagccagc agataatcac tgctggcgtg
     5221 gcgattaaca ccaatactca gtcacacatg atgtccgcaa atcagtagtc agaatgactc
     5281 ccctcccact gcatcagttc ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc
     5341 aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg aatggggcac
     5401 ctccttgctt catcgttagg aattattgac ttaatatagg gaaaataaaa ttgctgtctt
     5461 ttgcgcagga gttccccttt tatgccatct gctcgtctgc aacaacagtt catccgcctg
     5521 tggcaatgct gcgagggtaa atcgcaggac acaacgctca acgaactggc agcgttattg
     5581 agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg cggctggctg
     5641 acgtgggaag cggaagtcgg gcgtggtaaa cgctcgcgtc tgacattcct ctataccggg
     5701 ctggcgcttc agcaacagcg ggcggaagac ctgctggagc aggaccgtat cgaccaacta
     5761 gtgcagttag ttggcgacaa agcgactgtg aggcaaatgc tggtttctca tctgggccgc
     5821 agcttccgcc aggggcggca catcctgcgc gtgctctact atcgtccgtt gcgtaatctg
     5881 ctacctggca gcgcattgcg ccgttccgaa acccatatcg cccggcaaat cttcagttcg
     5941 ctaacgcgca taaatgagga aaatggggaa ctggaagcag acatcgccca ccactggcag
     6001 caaatatcac cgcttcactg gcgtttcttt ttgcgtccag gagtccattt tcatcatggt
     6061 cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc
     6121 tattcgcata ttgctgacat tgtgtcgccg acgccctgga cgctggatat ccacctcacg
     6181 cagccggatc gctggttgcc gttactgctg ggacaagttc cggcgatgat cctgccgcgc
     6241 gaatgggaaa ccctcagtaa ctttgccagc catcccatcg gcaccggtcc gtatgcggtg
     6301 attcgcaaca gcaccaatca actgaaaatt caggcattcg atgacttctt cggttaccgg
     6361 gcattaatcg atgaagttaa cgtctgggtt ctgccggaaa ttgccgacga gccagccgga
     6421 gggctgatgc taaaaggtcc acagggcgag gaaaaagaga ttgaaagccg cctggaggaa
     6481 ggttgctact atttactgtt cgatagccgc acccatcgcg gggcgaatca gcaagtcagg
     6541 gactgggtaa gctatgtgct ttctccaact aatctggtct atttcgctga ggaacagtac
     6601 cagcaactgt ggttcccggc ttatggactg ctcccccgtt ggcatcatgc ccgcaccata
     6661 acgagcgaaa aaccggctgg tctggaaagc ctcaccctga ccttttatca ggatcacagt
     6721 gagcatcggg tgattgccgg gatcatgcag cagattctgg caagccacca ggtcacactg
     6781 gaaatcaaag agatcagcta cgatcagtgg catgaaggag agatcgagag cgatatctgg
     6841 cttaacagcg ccaactttac gctgccgctg gatttttcgc tgttcgcgca cctgtgcgag
     6901 gtaccgctgc tccaacactg tattccaatc gactggcaag tcgacgccgc ccgctggcgc
     6961 aatggcgaaa tgaacctggc gaactggtgc caacaactgg tcgccagcaa agcaatggtg
     7021 ccacttatcc accactggct gatcattcag ggacaacgca gtatgcgcgg cctgcgcatg
     7081 aacaccctcg gctggtttga ttttaaatca gcgtggtttg cgccgccgga tccagagtag
     7141 cttgcgcctt ttggcggtca tgagcgcatt ttttatattc agacggaatg ataaaataca
     7201 ttacttttat attttctctg ttttatgcat cgtctgatta gcgtcgcaaa taagattaaa
     7261 gaaggggcat tccagcccct ctttcatatt ctgagttaat caataacggc gcagtctagc
     7321 cttgttctgg ccggaaaggt cagctttttc aggttaacct cgcgcaggtc agtcatacca
     7381 acggctattg ttcgtttcag cattgctcag gtcaacctcg agacagtcaa taccacaaat
     7441 gacaaccccg ttaaagtaag cattactcag atcaatcccc ccggtcctgg tgctcttttt
     7501 cagctgcaac tccagagtaa atatcgcctc atcgccaata aaaaaactga cgcataacgt
     7561 accttctggc gagagtgagg ccttcgcggc catattttcc ggatcttttg cgtattcttt
     7621 gagttcatta aattttatca gccagtgagt taacttatca cgcgccttcc cgtcgacagc
     7681 cccggtaaag tattttgagt agtcatcact attctggaaa taaaatgcat ccttcagaat
     7741 ttgccttgcc gcagtctggg aatgtttatc tgactggaag agattgcgta acgtctccca
     7801 tagctcttca aagatattca tattctcggt ctttttgacc aagttagcca tcctgataat
     7861 ctgagaatta gtaaggcctg gatctttagt ggtattacta gcagggatac taatctgttc
     7921 tgggtttctt actttcatgc tgatagctcc actctgaata aaaaactcag aataagcgtc
     7981 ctgaataaga atactgtcaa aaatcgctca ttcacgcaat aaacggttac gttacctatt
     8041 ttgactgtag cgaagggatt ctttcggatc aacgctctcc agctatgcaa atacgctctt
     8101 gatagctgaa cgaatattgg cttttttaat acgccttctc tatggattgc tggttgataa
     8161 caaaatcact acactaacgc cgttctcaac ggggtgccac gcggacgcgt gcgctgagaa
     8221 aatacccgtc gaacctgatc cggataacgc cggcgaaggg atttgaggct ccttctcaag
     8281 tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa atgtcttccc ctgctgttgc
     8341 tgtgcacagc gcccgttttc gctaaacccg ttctgactgt ttatacctac gattccttcg
     8401 ccgccgactg ggggcctggt ccggtggtta aaaaagcctt tgaagccgac tgtaattgcg
     8461 aactgaaact ggtggcgctg gaagatggcg tttcgcttct caaccgtcta cggatggaag
     8521 gcaaaaacag taaagccgat gtggtgctgg ggctggataa caatctgtta gacgccgcca
     8581 gtaaaaccgg actgtttgcc aaaagcggtg tggcagcgga tgccgttaac gttcccggcg
     8641 gctggaataa tgacattttc gtaccgtttg attatggcta cttcgccttc gtttatgaca
     8701 agaacaaact gaaaaacccg ccacaaagcc tgaaagaact ggttgagagc gatcaaaact
     8761 ggcgggtgat ttatcaggat ccgcgcacca gtacaccggg gctgggtctg ttgctatgga
     8821 tgcaaaaagt ctatggcgat gacgccccac aagcctggca gaaactggcg aagaaaacgg
     8881 tcacggtcac caaaggctgg agcgaagcct acggcctgtt tttaaaaggt gaaagcgatc
     8941 tggtactgag ttacaccacc tctccggctt atcacattct cgaagagaag aaagataact
     9001 acgccgccgc gaacttcagc gaaggtcact atctgcaagt ggaagtcgcc gctcgcaccg
     9061 ctgccagcaa gcagccggag ctggcgcaaa aattcctcca gtttatggtt tctccggctt
     9121 tccagaatgc gatcccaacc ggcaactgga tgtatccggt ggcaaacgtc acgctgcctg
     9181 ccggttttga aaaattgacc aaacccgcaa ccacgctgga gttcacgcca gccgaagtgg
     9241 cggcacaacg tcaggcatgg attagcgaat ggcaacgcgc cgtcagccgt taattcccgg
     9301 ctggttaatt ccaggtgtaa gcgccgccac gctggtggta gcggttgcgc tggcggcgtt
     9361 tctcgccctg tggtggaacg cgccgcaggg tgactggtcg gcagtctggc aggacagcta
     9421 cctgtggcat gtggtgcgct tctccttctg gcaggcgttt ctctcggcgc tactctctgt
     9481 cgtacccgcg atattcctcg cccgcgcgct ctatcgcagg cgctttccgg gtcggctggc
     9541 gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg ctggttgctg ttttcggcat
     9601 tcttagcgtc tatggtcgcc agggctggct ggcatcgctc tgccaatcgc tcggtctgga
     9661 gtggaccttt tcgccctacg gcctgcaagg cattttgctg gcccatgtgt tttttaatct
     9721 gccgatggcg agccgcttat tactccaggc actggaaaac attcccggcg aacaacgtca
     9781 gcttgccgcc cagcttggga tgcgcggctg gcattttttc cgcttcgtcg aatggccgtg
     9841 gttacggcga caaatcccgc cggttaccgc gcttatcttt atgctctgtt tcgccagctt
     9901 cgccaccgtg ttatcgctgg gcggcggtcc gcaggcaacc actatcgagc tggcaatcta
     9961 tcaggcgctg agttacgact acgatcctgc ccgcgcggcg atgctggcgc tgatccagat
    10021 ggtgtgttgc cttgggctgg tgctgttgag tcagcgactg agtaaggcca ttgcgcccgg
    10081 caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt ctgcatagcc gcatttgcga
    10141 cacgatgtta attgtgctgg cgctgctgct gttgctgcca ccgttgctgg cggtgatcgt
    10201 cgatggggta aatcgccagt tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct
    10261 gtggacctcg ttgcgtattg cgctggcggc aggtgtattg tgcgtagtgc tgaccatgat
    10321 gctgctatgg agcagtcgcg aactgcgggc gcggcaaaaa atgctggcgg gccaggcgct
    10381 ggagatgagc ggcatgttga tcctcgctat gccggggatt gtgctggcaa cgggcttctt
    10441 tttactgctc aacaacacta tcggcctgcc acaatctgct gacggcattg tgattttcac
    10501 caatgcgtta atggcgatcc cctacgccct gaaagtgctg gaaaacccga tgcgcgatat
    10561 caccgcccgc tacagcatgt tatgtcagtc gctggggatt gaaggctggt cacgcttaaa
    10621 agtagtggag ctgcgcgccc tgaaacgccc gctggcgcag gcgctggcct ttgcctgcgt
    10681 gctgtcgatt ggtgattttg gcgtggtggc gttgttcggt aacgatgatt tccgcaccct
    10741 gccgttttat ctctaccagc aaattggctc ctatcgcagc caggacggcg cggtcaccgc
    10801 gttaattctg ctgctgctct gttttctgct gtttagcgtg attgaaaaaa taccggggcg
    10861 aaatgttaaa actgactgat atcacctggc tttatcacca tttgccgatg cgttttagct
    10921 taacggtaga acgcggcgag caggtggcga tcctcgggcc aagcggcgcg ggtaaaagta
    10981 ccctgctgaa cttgatcgcc ggttttctga caccagccag cggtgcgctg actatcgacg
    11041 gcgttgatca caccacgacg ccgccgtcac gccgtccggt gtcgatgctg tttcaggaga
    11101 acaacctgtt cagccacctg acggtcgcgc agaacatcgg gctggggctt aatccaggat
    11161 tgaagctgaa cgcggtacag caggagaaaa tgcacgcgat tgcccgtcag atggggattg
    11221 ataatttaat ggcgcggtta ccgggcgagc tttccggcgg tcagcgacag cgcgtggcgt
    11281 tagcgcgttg tctggtacgc gaacagccga ttttattgct cgatgaaccg ttctctgcgc
    11341 tcgatccggc gctacgtcag gagatgttga cgctggtgag cactagctgc cagcagcaaa
    11401 aaatgacgct attgatggtg tcgcacagtg tggaagatgc ggcgcggatt gccacgcgct
    11461 cggtagtagt cgccgacggg cgaatcgcct ggcagggtat gaccaatgag ttgttgagcg
    11521 gtaaggcgag tgcttcggcg ctgttgggaa ttacaggtta atatgcactg tcggatgcgg
    11581 cgtgaacgcc ttatccgacc tacgggtgcg ttttgtccgc atcaagcgcc ccacacaaga
    11641 ctatgcccta aactccaacc actttacgca aaatatcgat atacaccggc attaacggat
    11701 ggcgaactaa caccaccagc gccaccacgc cgatggcaga aatcaacggc gtcagccaca
    11761 acaaacgacc gcgggacaaa taatgactca aacgatcggt tgctttgccg ctgcgccata
    11821 accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt
    11881 taaactcacc gctctgcatt ccggctggaa tatcgatcgc cgcacccgcc agaatccccg
    11941 gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgtg ataaattttg
    12001 ccaccgggag atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa
    12061 aacgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga
    12121 gtaatgcttt gttcttcttc agaaatgacc aacgatgcaa tggcttttta aaacgccagc
    12181 ccagccagaa ggagatccag tcgcccagca gacaaccgac gatccctgcc agccaggcgt
    12241 gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac
    12301 cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca
    12361 tcaatgaata cacggtggat tgggtaataa agtgttccag caatgcttgc ataatgtgcc
    12421 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
    12481 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
    12541 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg cgagaaatag
    12601 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtagtgctc
    12661 aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac gctaatccct
    12721 aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg ctgtgcgacg
    12781 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
    12841 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
    12901 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
    12961 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
    13021 aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
    13081 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
    13141 atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga
    13201 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
    13261 ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa acccgccacc
    13321 agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
    13381 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag atattgccgt
    13441 cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct tattaaaagc
    13501 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
    13561 cacggcagaa aagtccacat tgataatttg cacggcgtca cactttgcta tgccatagca
    13621 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
    13681 tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt
    13741 tggcagtgat tctgtgcgag ctttggcggt ggactgcacc accggtgaag aaatcgccac
    13801 cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca
    13861 gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct
    13921 tgcagaactt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg
    13981 ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc
    14041 cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga
    14101 agagattacc cgtttgtgcc acgcgccggg caatgttgac tactcccgct atattggcgg
    14161 tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc
    14221 cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc
    14281 cggtaccacg cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataagtcgct
    14341 atggcacgaa agctggggcg gcctgccgcc agccagtttc tttgatgagc tggatccgat
    14401 cctcaatcgc catttgcctt ccccgctgtt cactgatacc tggactgccg atattccggt
    14461 gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc
    14521 cggcggcgcg tttgactgcc atatgggcgc agttggtgca ggcgcacagc ctaacgcact
    14581 ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg
    14641 cgagcgggca gtgaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
    14701 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggttcg gtcgcgtact
    14761 cggctggccg ctggaacagc ttgccgccca gcatccggaa ctaaaagagc aaatcgacgc
    14821 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
    14881 tcacctgccg gtggtgctcg actggtttaa cggccgccgc acaccgaacg ctaaccaacg
    14941 cctgaaaggg gtgattaccg atcttaacct cgctaccgac gctccgctgc tgttcggcgg
    15001 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
    15061 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
    15121 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accaatgctg
    15181 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc
    15241 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc
    15301 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
    15361 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttccgactc tataaggaca
    15421 cgataatgac gatttttgat aattatgaag tgtggtttgt aattggcagc cagcatctgt
    15481 atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtg aaagcgctga
    15541 atacggaagc gaaactgccc tgcaaactgg tactgaaacc gctgggcacc acgccggatg
    15601 aaatcaccgc tatttgccgt gatgctaatt acgacgatcg ttgcgctggt ctggtggtgt
    15661 ggctacacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
    15721 cgttgctgca attccacacc cagttcaacg cggcgctgcc gtgggacagc atcgatatgg
    15781 actttatgaa cctgaaccag accgcgcacg gcggtcgcga gttcggcttc attggcgcgc
    15841 gtatgcgtca gcaacatgcc gtggttaccg gtcactggca ggataaacaa gcccatgagc
    15901 gtatcggctc ctggatgcgt caggcggtat ctaaacagga tacccgtcat ctgaaagtct
    15961 gccgatttgg cgataacatg cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac
    16021 agatcaagtt cggtttctcc gtcaatacct gggcggttgg cgatctggtg caggtggtga
    16081 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
    16141 tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
    16201 ttgagctggg gatgaaacgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
    16261 ttgaagattt gcacggtctg aaacagcttc ctggtctggc cgtacagcgt ctgatgcagc
    16321 agggttacgg ctttgcgggc gaaggcgact ggaaaactgc cgccctgctt cgcatcatga
    16381 aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact
    16441 tcgagaaagg caatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgatcg
    16501 ccgcagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
    16561 ctgcccgcct gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ttgattgatc
    16621 tcggcgatcg ttaccgtcta ctggttaact gcatcgacac ggtgaaaaca ccgcactccc
    16681 tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca accggatctg ccaactgctt
    16741 ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
    16801 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
    16861 acacccgcct gccagcgttt aaagacgcgc tgcgctggaa cgaagtgtat tacgggtttc
    16921 gccgctaagt agccgcatcc ggtatgtaac gcctgatgcg acgctggcgc gtcttatcag
    16981 gcctacacgc tgcaattttg taggccggat aagcaacgcg catccggcat tcagcgcctg
    17041 atgcgacgct gacgcgtctt atctggccta cacgctgcga ttttgtaggc cggataagca
    17101 acgcgcatcc ggcacgaagg agtcaacatg ttagaagatc tcaaacgtca ggtattagaa
    17161 gccaacctgg cgctgccaaa acacaacctg gtcacgctca catggggcaa cgtcagcgcc
    17221 gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta cagcatcatg
    17281 accgctgacg atatggtcgt ggtcagcatc gaaaccggtg aagtggttga aggtgcgaaa
    17341 aagccctcct ccgatacgcc aactcaccga ctactctatc aggcattccc gtccattggc
    17401 ggcattgtgc acacgcactc gcgccacgcc accatctggg cgcaggcggg tcagtcgatt
    17461 ccagcaaccg gcaccaccca cgccgactat ttctacggca ccattccctg cacccgcaaa
    17521 atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa
    17581 accttcgaaa aacagggtat cgatgcagcg caaatgcccg gcgttctggt ccattctcac
    17641 ggcccatttg catggggcaa aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa
    17701 gaggtcgctt atatggggat attctgccgt cagttagcgc cgcagttacc ggatatgcag
    17761 caaacgctgc tggataaaca ctatctgcgt aagcatggcg cgaaggcata ttacgggcag
    17821 taatgactgt ataaaaccac agccaatcaa atgaaaccag gctataatca agcctggttt
    17881 tttgatggat tttcagcgtg gcgcaggcag gttttatctt aacccgacac tggcgggata
    17941 ccccgcaagg aacagaggtc tccttctggc tggcgacgga caacgggccg ttgcaggtta
    18001 cgcttgcacc gcaagagtcc gtggcgttta ttcccgccga tcaggttccc cgcgctcagc
    18061 atattttgcg gggtgaacaa ggctttcgcc tgacgccgct ggcgttaaag gattttcacc
    18121 gccagccggt gtatggcctt tactgtcgcg cccatcgcca gttgatgaat tacgaaaagc
    18181 gcctgcgtga aggtggcgtt accgtctacg aggccgatgt gcgcccgcca gaacgctatc
    18241 tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac aatggcgcta
    18301 tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag tgggtttcta
    18361 ttgatattga aaccacccgc cacggagagc tgtactgcat cggcctggag ggctgcgggc
    18421 agcgcatcgt ttatatgctg ggaccagaga atggcgacgc ctccgcgctc gattttgaac
    18481 tggagtacgt cgccagccgc ccgcagctac tggaaaaact caacgcctgg tttgcaaact
    18541 acgatcctga tgtgatcatc ggttggaacg tggtgcagtt tgatctgcga atgctgcaaa
    18601 aacatgccga gcgttatcgt attccgctgc ggctggggcg cgataacagt gagctggagt
    18661 ggcgcgagca cggctttaaa aacggcgtct tttttgccca ggctaaaggt cggctaatta
    18721 tcgacggtat cgaggcgctg aaatccgcgt tctggaattt ctcttcattc tcgctggaaa
    18781 ctgtcgctca ggagctatta ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg
    18841 acgaaattga ccgccgtttc gccgaagata aacctgcgct ggcaacttat aacctgaaag
    18901 attgcgagct ggtgacgcag atcttccata aaactgaaat catgccgttt ttgctcgaac
    18961 gagcaacggt gaacggcctg ccggtggatc gacacggcgg gtcggtggca gcgtttggtc
    19021 atctctattt tccccgtatg catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc
    19081 cgccgcacgc cagccctggc ggctacgtga tggattcgcg acctgggctt tatgattcgg
    19141 tgctggtgct ggactacaaa agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc
    19201 ccgtcgggct ggtggaaggc atggcgcagc ctgatccaga gcacagcacc gaaggctttc
    19261 tcgatgcctg gttctcgcga gaaaaacatt gcctgccgga gattgtgact aacatctggc
    19321 acggacgcga tgaagccaaa cgccagggta acaaaccgct gtcgcaggcg ctgaaaatca
    19381 tcatgaatgc cttttatggc gtgctcggca ccaccgcctg ccgcttcttc gatccgcggc
    19441 tggtatcgtc gatcaccatg cgtggtcatc agatcatgcg gcaaaccaaa gcgttgattg
    19501 aagcgcaggg ctacgacgtg atctacggcg ataccgactc aacgtttgtc tggctgaaag
    19561 gcgcacattc ggaagaagaa gcgacgaaaa tcggtcgtgc actggtgcag cacgttaacg
    19621 tctggtgggc agaaacgctg caaaaacaac agctgaccag cgcactggaa ctggagtatg
    19681 aaacccattt ctgccgtttt ctgatgccaa ccattcgcgg agccgatacc ggcagtaaaa
    19741 agcgttatgc cgggctgatt caggagggcg acaaacagcg gatggtgttt aaagggctgg
    19801 aaaccgtgcg tacggactgg acgccgctgg cccagcagtt tcagcaggag ctttacctgc
    19861 gtatcttccg caacgagcca tatcaggagt acttccgcga aaccatcgac aaactgatgg
    19921 cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat
    19981 atcagcgtaa tgttccgcct catgtacgcg ccgctcgtct tgccgatgaa gaaaaccaaa
    20041 agcgcggtcg ccccttgcaa tatcagaacc gcggcaccat taagtacgta tggaccacca
    20101 acggcccgga gccgctggac taccaacgtt caccactgga ttacgaacac tatctgaccc
    20161 gccagctaca acccgtggcg gagggaatac tcccttttat tgaggataat tttgctacac
    20221 ttatgaccgg gcaacttggg ctattttgag caaaaaaaga gttcgccaga taccattttg
    20281 atgcgtgacg aatgctttgc catccagtac catagcgccc tttccattcc tggacctgaa
    20341 taacaccact acctcataag cacggtagcg ggtggttatt gcctgcaatt aaagatatag
    20401 agccgaacac atatgccttt tacacttggt caacgctgga tcagcgatac agaaagcgaa
    20461 ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg tcactttact tttcccatct
    20521 actggtgaaa accgtctgta cgcacgcagt gattcccccg tgacccgcgt gatgttcaac
    20581 cctggtgata ccattaccag ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa
    20641 gaaaatggct tgctgaccta tatcggtact cgcctggata ctgaagagtc cggcgtagcc
    20701 ctgcgtgaag ttttccttga tagcaaactg gtgttcagca aaccgcagga ccgcctgttt
    20761 gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc
    20821 agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg
    20881 caccagctca acatcgctca tgatgttggc cgccgccacg cgccgcgcgt cctgctggct
    20941 gacgaagtgg gtttagggaa aaccattgaa gccgggatga tccttcatca gcaactgctc
    21001 tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa ccttacagca tcagtggctg
    21061 gtagaaatgc tgcgccgttt caacctgcgc tttgcgctgt ttgatgatga gcgttatgcc
    21121 gaagctcagc acgatgctta caacccgttc gacaccgaac agctggtgat ttgctcgctg
    21181 gattttgccc gccgtagcaa acagcgtctg gaacatctct gtgaagccga gtgggacctg
    21241 ctggtggtcg atgaagcgca tcacctggtg tggagcgaag acgcgccgag ccgcgaatat
    21301 caggctattg aacaactggc agagcacgtg ccgggcgttc tgctgctgac cgcaaccccg
    21361 gaacagctgg gaatggaaag ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc
    21421 cacgatttcg cgcaattcgt tgaagagcag aaaaattatc gtccagtagc ggacgccgtt
    21481 gccatgctgc tggcaggtaa caaactgagc aatgacgaac tgaacatgct tggcgagatg
    21541 atcggcgagc aggatatcga gccgctgttg caagcagcaa acagcgacag cgaagatgcc
    21601 cagagcgccc gtcaggagct ggtttcgatg ctgatggatc gccacggcac cagccgcgtg
    21661 ctgttccgta acacccgtaa cggtgtgaaa ggcttcccga aacgcgagct gcacaccatt
    21721 aagctgccgc taccgacgca gtatcagacg gctattaaag tctccggcat tatgggcgca
    21781 cgtaaaagtg cggaagaccg cgcccgcgat atgctctacc cggagcgtat ttatcaggaa
    21841 tttgaaggtg ataacgccac ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc
    21901 tacctgacca gccatcgctc tcagaaagtg ctggtgatct gcgctaaagc tgccactgcg
    21961 ctgcaactgg agcaagtact gcgcgaacgt gaaggtattc gcgctgcggt gttccacgaa
    22021 ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga agacaccggc
    22081 gcacaggtac tgctgtgctc agaaatcggt tctgaaggac gtaacttcca gttcgccagc
    22141 cacatggtga tgtttgacct gccattcaac ccggatctac tggagcagcg tattggtcgt
    22201 ctggatcgta tcggccaagc gcacgatatt cagatccatg tgccttatct ggagaaaacc
    22261 gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg atgcatttga gcacacctgc
    22321 ccgaccggac gcactattta cgatagcgta tacaacgatc tgattaacta tctggcttca
    22381 ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact gccgcgagca acatgaagcg
    22441 ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg aaatccactc caacggtggc
    22501 gaaaaagccc aggcactggc agaaagcatt gaagagcagg atgacgatac caacctgatc
    22561 gccttcgcca tgaacctgtt cgatattatc ggtatcaatc aggacgatcg cggcgacaac
    22621 atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg acttccctgg cctgtcggaa
    22681 gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg cgcgtgaaga tgcgcagttt
    22741 attacctggg aacatccgct gatccgcaac ggtctggatc tgattctttc tggcgatacc
    22801 ggtagcagca cgatttcact gttaaaaaac aaagcgttgc cggtaggtac gctgttggtg
    22861 gaactgattt atgtggttga agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg
    22921 ccaccgacgc cggtacgtat gctgctggat aaaaacggca acaacctggc ggcgcaggta
    22981 gagtttgaaa cctttaaccg ccagcttaac gcggttaacc gtcacaccgg cagcaaactg
    23041 gttaacgccg tgcagcagga tgttcacgct atccttcaac tgggtgaagc gcagatcgag
    23101 aaatctgccc gtgcattgat tgatgcagca cgtaacgaag ccgacgaaaa actgtctgcc
    23161 gagctgtctc gtctggaagc tctgcgtgca gtgaacccga acattcgtga cgacgaactg
    23221 accgccattg agagcaaccg tcagcaggta atggaaagcc tggatcaggc aggttggcgt
    23281 ctggatgccc tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa atggggatgg
    23341 aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat gaccatatta
    23401 tggtggtcaa caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag
    23461 acagcgtgat gacgcgcatt cagcgagatt atccgcaggc agaatcggtg catcgtctgg
    23521 atatggctac cagcggcgtg attgtagtgg cgctgaccaa agccgcggag cgggagttaa
    23581 aacgccagtt ccgcgagcgc gtgccgaaaa agcagtatgt gacccgcgtc tgggggcatc
    23641 catcccccgc agaaggtctg gtggatctgc cgctgatttg cgactggcca aaccgcccga
    23701 aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa gtggtggagt
    23761 atgcggcgga taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcaat
    23821 tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg cgatcgtttt tatgcatcac
    23881 cagaagcgag agcgatggca ccgcgtttgt tgctacatgc agagatgttg acgattaccc
    23941 atccggcgta tggcaatagt atgacgttta aagcgccagc ggatttttaa cctttcctgt
    24001 acgtttatac cctcatccta agggctgcct cttgtacata aatccccgag tccggggatt
    24061 ttttgtttct gccaaactgc tttcagttgt tgcgtgtggc tacgctttcc attcggtaat
    24121 tcccttcctg ctccacagcg aatttttgaa ctttttcttc acccgttgac cgtctcttat
    24181 atttcatttt atcgggtctg ttatggtcct ttctgattgt tattcatggg ctaattagca
    24241 gtctggttgg cgatgcccat ctcggtgatc cgcgtcgtac acgacgcctt gcgtcactag
    24301 cctcttctct agctcatcat gcaggtcttt ctaattgtga aatcatccca cgctactgct
    24361 aaggttgaag gggcgttcca gccccttcaa ccttagctta ttctggctca ttcaataatt
    24421 gcgttgtcag taatttgttc agcagtactt tgtttagtag caacttcttc agtatcaatt
    24481 tttttggggg tgattttatt aataataatt acctcatcat ataggtgggt agcaccgtcc
    24541 acggtagcac cgtccacggt agcaccgtcc agggtagcac cgtccaggtt agcattccac
    24601 ccaggttcca agtaaaggac tttattgtga cctttcattg ttttgagacc aaggtaacaa
    24661 tccctcatgt caacgccggg aaggtaagcc tctctaagat caacgcccaa gttacctggg
    24721 cctttatgca gctgcaacgt ctgagtacat atctccttat tttcaataaa aaaagtgaca
    24781 cttaactcga tatcaccgaa ggtctgtgcg acccagctca tattgccctt tgcgaccatc
    24841 tcatccggat tttttgcgca tgttttgagt tcattgaata attccagact atggataaac
    24901 ttttctttct cattctcgtt gacagcccca gtaaagtatt ttgaggcgtt gatgttgcca
    24961 gcctttttat tcacgaaatt gaacagaagt ttataagcct cagtctgttt atctgaccag
    25021 aacagatgac gtaacgtctc ccacaagcgt tgaaagatat tcatcttcat gtccatcttg
    25081 tccccgctca ccatcttata aatatcgtta ttgctcatat ttaaaagtgt agccggcatg
    25141 cctggaactg atactttcat ggtaatagcc tcgcattgag ttaaaaactc atgatgcgcg
    25201 ctctgaataa atatactttc aaaaatcgct cattcagata tgtgtttttt atttttaaga
    25261 cgtaaaaata taattccgct cgccgtaaag ctctcaacct taagcagggt caacgcgata
    25321 aaaccaacag aaaccctccc gtaaaccgta tattgagcct gtccaccatt ctgagtaaac
    25381 gccttttctc aaagaagtga ccgccagggc ggccaccttt tatcgtgggc agttactcag
    25441 catcatttac agggtcattt aaaccctttc tgctgcttaa taagctcata ggcctgctga
    25501 atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
    25561 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
    25621 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc
    25681 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
    25741 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
    25801 atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
    25861 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
    25921 tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcattttgc
    25981 gccgcagtac gggaagcgcc atgaagattc attcggtcca tcaactggct ggcgatatga
    26041 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
    26101 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
    26161 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
    26221 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
    26281 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
    26341 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
    26401 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
    26461 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
    26521 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
    26581 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
    26641 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctaaccacgc
    26701 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
    26761 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga
    26821 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
    26881 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggaaggtga
    26941 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
    27001 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
    27061 ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
    27121 gaacgcccgc tttaaggtgg gtccggtatc gatcttttat agcccctatt tgcagttgcc
    27181 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
    27241 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
    27301 cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat tccgctacct
    27361 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
    27421 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
    27481 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
    27541 cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
    27601 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
    27661 tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg ttaattacta
    27721 ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc actttgttaa
    27781 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
    27841 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
    27901 gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg aatccgttaa
    27961 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
    28021 gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt acgtgccgta
    28081 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
    28141 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
    28201 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
    28261 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
    28321 tgacgacaaa acgggttcac tggtgtgggc aggcgatact tactggcgta tctccgagcg
    28381 ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag cgaccagtaa
    28441 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
    28501 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
    28561 gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc gttggtccat
    28621 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
    28681 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
    28741 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
    28801 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
    28861 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
    28921 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
    28981 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
    29041 tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct gctcaggcaa
    29101 ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
    29161 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    29221 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    29281 gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    29341 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    29401 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    29461 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa
    29521 gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    29581 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    29641 aggagttgcc cgggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    29701 gcccgattcg ttccggcgtt ggcttccata tactgaaagt taacgacctg cgcggcgaaa
    29761 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    29821 tcatgactga cgaacaggct cgtgtgaaac tggaacagat tgctgctgat atcaagagtg
    29881 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    29941 agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc cgtgacgccc
    30001 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    30061 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    30121 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    30181 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    30241 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    30301 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    30361 gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg cacaaccgca
    30421 aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    30481 gttagcgatt gaaaatgggc attacgtggt ggagacgctg gcgcgagcgt gtgatggctg
    30541 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    30601 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    30661 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    30721 gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga ttgctatttt
    30781 gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    30841 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    30901 tgtgctcgac gagctgcggg cgcaggggat gaaactcaac gggccgctgc ctgccgatac
    30961 cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt accacgatca
    31021 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    31081 gccctttatt cgcacatcag tggaccacgg caccgcactt gaactggcgg gacgtggcga
    31141 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    31201 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    31261 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca aaagggccag
    31321 gcaatggtcg aaatcggccc cggtctggcg gcattgaccg agccggtcgg cgaacgtctg
    31381 gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    31441 ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa ctttggcgaa
    31501 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    31561 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    31621 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc gtatggtcga
    31681 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt tccgccgtca
    31741 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    31801 atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga agcctttaac
    31861 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    31921 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    31981 atggcgaact atctggcgga gaacgcgcct tttcaggaga gttaacgatg atcaattcgc
    32041 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    32101 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    32161 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    32221 aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag tacaccagcg
    32281 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    32341 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    32401 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    32461 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg
    32521 atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    32581 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcagta tttgccggga
    32641 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    32701 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    32761 tgatggccca cgcagggatc acgccgcagt gggatctgca gaccgccaaa gagtgcgcgc
    32821 gcgatgtaga agcggtgctg tcgagtgact cctatccctt ctttctcgac gctatgtacg
    32881 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cggtttatca
    32941 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    33001 aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt cctggacctg
    33061 tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    33121 cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta acctgcctgc
    33181 gctgggaaga taaacagtat tttgtccagc cgtcgaaccg gcataaggat ttgggtgagg
    33241 gagaggcggt cgcgtcttaa atcccgtcga gcataaaatc taatgctcct ttaatcaacg
    33301 ttcggtgcgc gctggcatcg caaaatttcg ttcccagcgc gttaactgga acagttgcag
    33361 tctcatgcgt catgagcaca tattctttgc cgtctaccag taaaaggatt gggttaagcc
    33421 gttctggtgg gcgaatacgg ctaaatcgtt caataggtgt taatggaata acaatacggc
    33481 gattaatcac accaataatg tcgctggtaa catcgataac aaagggaaac gcggcgttcc
    33541 tgctgcgact gcggtatacc gtaaattgca tcgctagaag ctccggtact catcagagaa
    33601 acatccggtt tcatcagcca attgatttaa cgcagcgata gcttcagcgt tctcttcacg
    33661 ccaacgtgtt gctgcatgtt ttttaagttc agcattgagc gcaaccgtaa gggtggcgct
    33721 catatttaag cctgcatcac gagcgcgatt gactaactca cggtcgacgg tgacggtcac
    33781 actttgtgtg gtacgttttg cagtcatgat atcgtgctcc tgtacaattt atacgtctag
    33841 tatacacgga tatctgcacg tatcagtgat gccggaattc taatatacaa aattaccgcc
    33901 gctccagaat ctcaaaacaa tagctgtgag agttctgcgc atcagcgtcg tggaattcgc
    33961 tgaataccga ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt
    34021 ctgcgtcgat atgcgtcagg tacagttttt gcgctttcgg caggaactgc tcataaacgc
    34081 gaccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcatcca
    34141 ccgacttcac ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt
    34201 tgcgtcctgg caacggacga ccgatggatt ccctggtatg gcggcccata attacgggtt
    34261 tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt
    34321 tttccatgcc gataacgcga tctaccgcta acgccgcaat cagactgatc attgagattt
    34381 cccgataaaa aaaattgtcg ccactatacg taaagcgtaa accgtcgtcg actggtgcga
    34441 ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc cacgctctct ccctggactc
    34501 gccgcattac aatgaaacaa aaacaaacag ttagctgtaa agtgtgattt acgtcactct
    34561 ttattaggat gagggtttcg tttccggttc atccgccatg ttgccggtat gtttaccttc
    34621 ttctgttccc tgccagccat gtcgttgaat taatgacaga tgttcgcggt cctcggtgat
    34681 gatctcactt aacatcgcgc tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt
    34741 ctcaaccatt gccatctctt ccaccatctg aatattaaag cggcggaaca catcggcacg
    34801 ttctcgcgct tcatacggcc ccagacctaa actttccagc gccagacgcc cggttttcag
    34861 cgcaccttca aaggtttcac gctctggctt ttcgacgcct gcctgacgca aacaaatgta
    34921 gtgatcgaca tcgcgggcgc gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat
    34981 ctccgtcagt tgcaggttgg tttgcggatc gtcaatggcg ttaatcagca cttccgcttt
    35041 cgccgctccc gcagattcca gtaaatccat ccgcgtggca tcgccataaa acactttcat
    35101 accaaattta cgcagcgttt cgatatggtc cggatcgtga tcgagcacca ccattttcac
    35161 cccgctggag agcagtaaac gtccggtgat ctgcccgaaa cgaccgaatc cggcaatgat
    35221 tacccgcggc tgttcttcgt cgatctcatc ggcttcacgc gcctcttcag tagaagattg
    35281 ctcaaggcga ttgaggatca ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc
    35341 cagggtcagc gatttcgccc actccggctc cagcacattc gccatctgcg ccgcgccaaa
    35401 taccacaaag gcaaactcac tgccctgccc taacaacacc gcaaaccagc gacgctgttt
    35461 atttggcacc tgcaacggtc gggcaatcag ccacagcatg gcgattttga tgatgaggaa
    35521 accgagcagc aaaatgacaa tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga
    35581 catgccaaca ccgatgaaaa acagccccaa cagcaaacct ttaaagggtt cgatatcgct
    35641 ttccagcgca tgacggtatt ccgagcttgc cagcagtacg cccgccagaa acgcgcccat
    35701 cgccatcgac aagccgacct cttccagcag caaaccaaag ccaaacacaa ggaataacgc
    35761 cacggcacta aacacttccc gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt
    35821 gacatagcgc cccagcagta ccaccagcac cagcgcgccc gccactttta acgccgagag
    35881 agcaaatgcg cccatcgtcg tcgaggcact gctcgccgcc agtagcggga tcatcgccac
    35941 cagcgggatt gccgcgatat cctggaacag cagcaccgca aaggcactgc ggcccatttg
    36001 cgtcaccatc agattgcgtt cattcatcgc ctgcatggca atcgccgtag aggagagcgc
    36061 cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag
    36121 ccccagcagg ccgccgcaaa tcaccatctg caatgcgcca ccgccaaaca ctgccgcacg
    36181 cagcttccac agcctttgtg gatcgagttc gaggccgata ataaacagca tcagcaccac
    36241 gccaatctcg gcaaagtgca gaatagattc ggcatcggtc accagtcgca gcccccacgg
    36301 gccaataatg cagccggcga tcaggtagcc aagtaccgat cccagaccaa gacgtactgc
    36361 aatgggtaca atcagcgccg ccgaaccgag ataaatcagc gcctgaacca gcgtatggct
    36421 atccatgatg ggcctcctgc cattccagca gacgttgctt atagtgacgc gcctgccctt
    36481 cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc aaacggtggc agccagttca
    36541 gcccgcagta gattgccgtc gcctgtagcg gctgcgacag cacatcaaag cccggatgcg
    36601 caccaatttc aaaatggctt tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc
    36661 catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg cgagaagact ttatcgatcc
    36721 aaagtttaag gagcggagga atgctgtacc actgcatcgg gtgctgccag acgatcagat
    36781 cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat attgaagtca ggatagagtt
    36841 gataaagaga gcgaatttcg acgccttcca gcgtccttgc ctgttcaaac atccgtttat
    36901 tcgcatggga atgatgcgga tacggatgcg cataaattat aagaatcata ggtagcccgc
    36961 gtgtttacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa
    37021 taatattgat tcagaaaaga gataaatctg aacaaattac cccattctct gcccgcggat
    37081 cgtcatattg ctggtctgcg ccagtgacat ccctcgagtc tccggggcaa acgctacgga
    37141 aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac tgataccgta
    37201 attattgata aagatcggta gtgcccaggt cgaaacaatg gtgccaatac gacttaagga
    37261 cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat taggatagag
    37321 ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg ccatcactac
    37381 cagccagatc cccatatccg ggatcagccc caaaaccgcc agcgccagcg tcatcatggc
    37441 aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca tcggcggaat
    37501 acagccgagc ataaagaaca ggctaatcac cacgttcccc agtgccgcgt ttttgccaac
    37561 acccaatccc aacaaaccaa cgatttgcgg gccaaaggtg taaatggcga acattgggat
    37621 cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa
    37681 cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa
    37741 cagtttgatc atcatctctt cgcactcttt tactcgccct ttgcgtaata accagcgggg
    37801 agattcaggc agttcgaatc gaccaatcaa aatcaacaaa caggggatcg ccgcgctacc
    37861 cagcatccag cgccagccgc cttccacatc ataaagccag tagccgacca gatcggcaca
    37921 ggtcgcgccg acataccaca tggcggcgat aaagctgatg gaaaacgccc gctgacgggt
    37981 actggagaac tcagtgatca tcgaagtggc gatgggatag tctgcaccga tgacaatgcc
    38041 gataagtacc cgcatcacca acagttcgac gggggatgaa acaaacatcg tcgccaccga
    38101 tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga ctttatcgga
    38161 aatataacca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa
    38221 gccaatccag tcagcgtcca gttttagcgc cggcgtcagt tgctccagcg ccacgccaat
    38281 cattaccagt atataaccat ccagaaacgg accaccgctt ccccacagca aaatgcggcg
    38341 gtgaatagag gaaaatttaa gatcgtcaaa gtttctggac ggttgcatgg gttattcctg
    38401 ttttttgcaa tgagagtcag tgcggcgtta cgtatcaaac caacatcagc cgtaacggaa
    38461 ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc ccagccccag
    38521 aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc
    38581 ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagcg cctgtttatc
    38641 ggcatcggct ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt tattgacgcc
    38701 cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt catgccatcc
    38761 ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt gcgcatcagt
    38821 tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcctaccgc cagttccggg
    38881 tagccgctaa acatgcgtgg gttatcaagg aaggccggta gtttctggta catacgcata
    38941 tcgcgcagcg ggccactctc aagatgctga cgatattccg cgagtttttg cttactgaaa
    39001 tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc ggcaatcgcc
    39061 agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc atcaccggca
    39121 atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc cggcactacg
    39181 tgagcggaat attccaccag cttgccaccc gcgatcagcg gtgcaacggc cggatgttgt
    39241 ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg atgcagacca
    39301 caaaccagcc ccagcgacag ggtgttttca ttggtataaa gaaaaccgcc gcccatcagg
    39361 ccatcggtgg gtgatcccgc aaataggcaa gccgccccct gattaccctg caactgaaaa
    39421 cggtcttcaa taaccgactt cggtaactcg atcagttcct tcacgccaac cgccacattc
    39481 gtcggtttaa cgcgttttgc catccccagc ttttcggcaa ggatggagtt cactccatca
    39541 gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg cttctacacc gacgactttg
    39601 ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct
    39661 tcggcctgct ccatcagcca ggcatcaaat ttactgcgca aaacggagta agaacgctgg
    39721 gatggcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt ttccgtcata
    39781 aacgcgagtt tttcatgggt gatcaggcgt tctaccgggg cggattcggc gaaaccagga
    39841 ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg
    39901 gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac cagtgcggca
    39961 accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc ggacataaga
    40021 gctccctgtc agcggcagga ccaaaatgca gccctgccag agtggatcaa cgcgctaaag
    40081 ctgcggtcag cgccggaagg atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact
    40141 ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac gcgttagcgc
    40201 caaccatgtg ctggatctgc ccggagatcc ccacagccag gtacagttca ggtttcagca
    40261 tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt tctgccaccg
    40321 gacgagaaca ggccaattcc gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt
    40381 tctctttgct gccaataccg cgaccgacgc tgaccaccag gcgggctttg tcgagatcga
    40441 cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc
    40501 actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc
    40561 cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca atcgccagac
    40621 caccgtaaac catatgtttc actgttgctt taccgtcctg tacgctgacg gtgctggcat
    40681 cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat ttgccgcgac
    40741 gggtgtttgg caacagcacc aggccgtctg caccgtgctg gcgaatagtg tcagccatga
    40801 cactggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc cagacatgat
    40861 tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag gcattgattt
    40921 gattagctaa agcctgcgca ccgttcatca gttccggcag acgagaaggg gtatcgctga
    40981 atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga ctttgcgaag
    41041 attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg
    41101 ctcgcgctgt ttcggcgcgg caacctgttg ttctgaccat gctgcctctg cgttaaaacc
    41161 gatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa tggctttcat
    41221 cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag gcagtggaat
    41281 gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat ctgccgtcag
    41341 ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc ctaccagcaa
    41401 accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca aatcaaagcc
    41461 tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa
    41521 ctggtcatca atcaccacaa tcagttcatc cggaccgcgc gatagcacat ctttacgccc
    41581 tttggcgttg gtcagggctt taccgcccac acttagggcc gtcacctgcg cctctgctgc
    41641 ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc ttattttggc
    41701 atcggcttta ctgaagtcta acgaaccatc agcattattg accgcaatat cctgttcatc
    41761 aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca tgaaggaaac
    41821 gtcgttcata tcggtggtgt taattacaga tatcatgccc tttaatttgg aaataaaact
    41881 ttaatcacca atattgaaaa tgtcacacgc agataaatcc aactttcaat a
//
LOCUS       DAZFKY010000019        40057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFKY010000019 DAZFKY010000000
VERSION     DAZFKY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHKCL_000937"
     CDS             complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHKCL_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5761959.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2090..2956
                     /gene="yghU"
                     /locus_tag="ACHKCL_000938"
     CDS             2090..2956
                     /gene="yghU"
                     /locus_tag="ACHKCL_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5761960.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="ACHKCL_000939"
     CDS             complement(3079..3327)
                     /gene="hybG"
                     /locus_tag="ACHKCL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5761961.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3340..3681)
                     /gene="hypA"
                     /locus_tag="ACHKCL_000940"
     CDS             complement(3340..3681)
                     /gene="hypA"
                     /locus_tag="ACHKCL_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5761962.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3674..4162)
                     /gene="hybE"
                     /locus_tag="ACHKCL_000941"
     CDS             complement(3674..4162)
                     /gene="hybE"
                     /locus_tag="ACHKCL_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5761963.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4155..4649)
                     /gene="hybD"
                     /locus_tag="ACHKCL_000942"
     CDS             complement(4155..4649)
                     /gene="hybD"
                     /locus_tag="ACHKCL_000942"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5761964.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4649..6352)
                     /gene="hybC"
                     /locus_tag="ACHKCL_000943"
     CDS             complement(4649..6352)
                     /gene="hybC"
                     /locus_tag="ACHKCL_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5761965.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6349..7527)
                     /gene="hybB"
                     /locus_tag="ACHKCL_000944"
     CDS             complement(6349..7527)
                     /gene="hybB"
                     /locus_tag="ACHKCL_000944"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5761966.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQHLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7517..8503)
                     /gene="hybA"
                     /locus_tag="ACHKCL_000945"
     CDS             complement(7517..8503)
                     /gene="hybA"
                     /locus_tag="ACHKCL_000945"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5761967.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8506..9624)
                     /gene="hybO"
                     /locus_tag="ACHKCL_000946"
     CDS             complement(8506..9624)
                     /gene="hybO"
                     /locus_tag="ACHKCL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5761968.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9813..10100)
                     /gene="yghW"
                     /locus_tag="ACHKCL_000947"
     CDS             complement(9813..10100)
                     /gene="yghW"
                     /locus_tag="ACHKCL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5761969.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10219..11106)
                     /gene="yghX"
                     /locus_tag="ACHKCL_000948"
     CDS             complement(10219..11106)
                     /gene="yghX"
                     /locus_tag="ACHKCL_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5761970.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11263..12303
                     /gene="gpr"
                     /locus_tag="ACHKCL_000949"
     CDS             11263..12303
                     /gene="gpr"
                     /locus_tag="ACHKCL_000949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5761971.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12343..12837)
                     /gene="yqhA"
                     /locus_tag="ACHKCL_000950"
     CDS             complement(12343..12837)
                     /gene="yqhA"
                     /locus_tag="ACHKCL_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5761972.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13028..13912
                     /gene="yghA"
                     /locus_tag="ACHKCL_000951"
     CDS             13028..13912
                     /gene="yghA"
                     /locus_tag="ACHKCL_000951"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5761973.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14184..14609)
                     /gene="exbD"
                     /locus_tag="ACHKCL_000952"
     CDS             complement(14184..14609)
                     /gene="exbD"
                     /locus_tag="ACHKCL_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5761974.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14616..15350)
                     /gene="exbB"
                     /locus_tag="ACHKCL_000953"
     CDS             complement(14616..15350)
                     /gene="exbB"
                     /locus_tag="ACHKCL_000953"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5761975.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15602..16789
                     /gene="metC"
                     /locus_tag="ACHKCL_000954"
     CDS             15602..16789
                     /gene="metC"
                     /locus_tag="ACHKCL_000954"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5761976.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16929..17588
                     /gene="yghB"
                     /locus_tag="ACHKCL_000955"
     CDS             16929..17588
                     /gene="yghB"
                     /locus_tag="ACHKCL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5761977.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17628..18527)
                     /gene="yqhC"
                     /locus_tag="ACHKCL_000956"
     CDS             complement(17628..18527)
                     /gene="yqhC"
                     /locus_tag="ACHKCL_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5761978.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18721..19884
                     /gene="yqhD"
                     /locus_tag="ACHKCL_000957"
     CDS             18721..19884
                     /gene="yqhD"
                     /locus_tag="ACHKCL_000957"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5761979.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19989..20816
                     /gene="dkgA"
                     /locus_tag="ACHKCL_000958"
     CDS             19989..20816
                     /gene="dkgA"
                     /locus_tag="ACHKCL_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5761980.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21016..21938
                     /gene="yqhG"
                     /locus_tag="ACHKCL_000959"
                     /pseudo
     CDS             21016..21938
                     /gene="yqhG"
                     /locus_tag="ACHKCL_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            21987..22244
                     /gene="yqhH"
                     /locus_tag="ACHKCL_000960"
     CDS             21987..22244
                     /gene="yqhH"
                     /locus_tag="ACHKCL_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5761981.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22287..24506)
                     /gene="ygiQ"
                     /locus_tag="ACHKCL_000961"
     CDS             complement(22287..24506)
                     /gene="ygiQ"
                     /locus_tag="ACHKCL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5761982.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24617..26029)
                     /gene="ftsP"
                     /locus_tag="ACHKCL_000962"
     CDS             complement(24617..26029)
                     /gene="ftsP"
                     /locus_tag="ACHKCL_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5761983.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26104..26841)
                     /gene="plsC"
                     /locus_tag="ACHKCL_000963"
                     /gene_synonym="parF"
     CDS             complement(26104..26841)
                     /gene="plsC"
                     /locus_tag="ACHKCL_000963"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5761984.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27075..29333)
                     /gene="parC"
                     /locus_tag="ACHKCL_000964"
     CDS             complement(27075..29333)
                     /gene="parC"
                     /locus_tag="ACHKCL_000964"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5761985.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29471..31078)
                     /gene="ygiS"
                     /locus_tag="ACHKCL_000965"
     CDS             complement(29471..31078)
                     /gene="ygiS"
                     /locus_tag="ACHKCL_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5761986.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31187..31669)
                     /gene="ygiV"
                     /locus_tag="ACHKCL_000966"
     CDS             complement(31187..31669)
                     /gene="ygiV"
                     /locus_tag="ACHKCL_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5761987.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31722..32114)
                     /gene="ygiW"
                     /locus_tag="ACHKCL_000967"
     CDS             complement(31722..32114)
                     /gene="ygiW"
                     /locus_tag="ACHKCL_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5761988.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32266..32925
                     /gene="qseB"
                     /locus_tag="ACHKCL_000968"
     CDS             32266..32925
                     /gene="qseB"
                     /locus_tag="ACHKCL_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5761989.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32922..34271
                     /gene="qseC"
                     /locus_tag="ACHKCL_000969"
     CDS             32922..34271
                     /gene="qseC"
                     /locus_tag="ACHKCL_000969"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5761990.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34381..34962
                     /gene="mdaB"
                     /locus_tag="ACHKCL_000970"
     CDS             34381..34962
                     /gene="mdaB"
                     /locus_tag="ACHKCL_000970"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5761991.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34993..35307
                     /gene="ygiN"
                     /locus_tag="ACHKCL_000971"
     CDS             34993..35307
                     /gene="ygiN"
                     /locus_tag="ACHKCL_000971"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5761992.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35352..36239)
                     /locus_tag="ACHKCL_000972"
     CDS             complement(35352..36239)
                     /locus_tag="ACHKCL_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5761993.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36236..37183)
                     /locus_tag="ACHKCL_000973"
     CDS             complement(36236..37183)
                     /locus_tag="ACHKCL_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5761994.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37194..38231)
                     /locus_tag="ACHKCL_000974"
     CDS             complement(37194..38231)
                     /locus_tag="ACHKCL_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5761995.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38240..39223)
                     /locus_tag="ACHKCL_000975"
     CDS             complement(38240..39223)
                     /locus_tag="ACHKCL_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5761996.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39220..40029)
                     /locus_tag="ACHKCL_000976"
     CDS             complement(39220..40029)
                     /locus_tag="ACHKCL_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5761997.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT         9970 a        10777 c        10057 g         9253 t
ORIGIN      
        1 cagtacggga ttgctacaac atgtattact ctttcaccac aattaacggt tcaatatcac
       61 tctctttttt aataaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
      121 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
      181 gctgatagat gtttttctgc tcggcaaatt taccactggt tttgtccagc acctcttcat
      241 gatggctgac gaggtcgatg ttgctgccac agcgaccggc gatcggtttc actgcgtaac
      301 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
      361 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
      421 gctcaaagac cagcacttcc gggcgcagca atacgtcgat aagacgcact tcattttgcg
      481 gatgaccggt acggatcggc accgcagcaa actcacggtc gctaacttca cgaatctgat
      541 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
      601 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
      661 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
      721 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
      781 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
      841 aacgttcgag gatcaagccc gcttcggtat gacaggaggc ggagtcggcg ttgtactcgt
      901 aaaccttcag accacgctca tccatgcaga aatccatacg accggtgatc atatggtgac
      961 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcggaatg tcgaacagcg
     1021 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc aggtgcagct
     1081 cgttggtggc tttaatcagc tcctgctcgg cgctctcggt aatggtgtag taatgatacg
     1141 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgcagc ggatcttttt
     1201 catccagcca tttaccgtca aactggcctt tgttttccag acgcgctccg ctgattttca
     1261 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
     1321 tccagcccag aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga
     1381 ccaccatctc cagttcgcgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
     1441 tctgttccgc aatacggact ttgttgccat gtaattgggt aatgatggcg acatggccag
     1501 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
     1561 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tcacgcagga
     1621 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtagttca
     1681 gaaagagaaa acggcgagca aattcaacgc attgccattt gtggcccata tattcgtcgt
     1741 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
     1801 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
     1861 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
     1921 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
     1981 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
     2041 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
     2101 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
     2161 tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg ctgccggtgg gcaaacatcc
     2221 attacaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
     2281 gctgctcgcg ctgggcgtta ctggcgcaga gtacgatgcc tggctgatac gcataggcga
     2341 tggtgatcaa ttctccagcg gctttgtcga agtgaaccca aattcgaaga tcccggcgct
     2401 gcgcgatcat acccataatc cgccgatccg cgtgtttgag tcaggatcga ttctgcttta
     2461 tctggcggag aaatttggct acttcctgcc gcaggatctg gcaaaacgga ctgaaacgat
     2521 gaactggctg ttctggttac agggcgcggc accgttcctc ggcggtggtt ttggtcactt
     2581 ttaccattac gcgccggtga aaattgagta cgccatcaat cgctttacca tggaagccaa
     2641 gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttgttg cgggcgatga
     2701 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aacgtggtgc tcggcggcgt
     2761 gtatgatgcc gcagagtttc tggatgcggg aagttataaa catgtgcaac gctgggcgaa
     2821 agaagtgggc gaacgtccgg cagtgaagcg tgggcgtatt gttaaccgca ccaacgggcc
     2881 gctgaacgag cagttgcatg agcgccatga cgccagtgat tttgagacga atacggaaga
     2941 taagcgtcag gggtaaggat tggtgttcgt cgcagcaagc catccaggcc ggataaggcg
     3001 ttcacgccgc atccggcaat cgagcacaat gcctgatgcg acgctgccgc gtcttatcag
     3061 acctacgaaa gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagtgcgtc
     3121 taatgtggct ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag
     3181 cacccactgg cccagcagat cggcagggtt accttcacaa atcagggcga tattcacgtc
     3241 gcgcttgata ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag
     3301 cacctggcct ggaacgccaa tacacattgt taactccggt tattcaactt caatactttt
     3361 gacgatcagc gaatcgccgg tatcgacacg caaccgctcg ccgtgacaga gcggacactg
     3421 cgcatcgtgc tgatgaacct ccaccacctg gctgcaatcc cagcaccagg cctgggcggg
     3481 tttatagacg atatgtaaat cgcacccttg cgccaccgtt ccgtggcaga caatttcaaa
     3541 actaaaacgg acggcgctct cctcaacgca ggagagcgcg ccaatttcca gccacacggc
     3601 ggtgacgcgc ttaacatcgt gctgctccgc ctgccgctgg ataatttcaa cggcgctctg
     3661 gcaaagcgac aactcatgca ttttcgccac tcctgcgacc aaacagcagg gcgcgacgcc
     3721 ctgcgtgtgg tacatccgga ttcgtgactg gcagcgaaag gatcattcgt gcgcagtcat
     3781 ccgtcaggcg ttggccctct tcaatcgaca tactgtgcga aagcggtgac atcagcgaac
     3841 aggagaggta ttgcgaaaca ccgtccagtt cgccaacggt aaaggtcatg gtgccatacg
     3901 gcagttgcag accaattttt tcactcactt tgcgcagcgg ccagagttga tccgggccgg
     3961 ggaagataac tgcactcagc atccacgggg tgatcacaca ccccgtccac tgaccttcga
     4021 acagcgtaaa atcagaaaca tacaccggca ttgaaggatg cagaaaagag agagcgtgca
     4081 tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg cgccttcggg gaggtctgga
     4141 aacctgctat ctcttcagtc atgaatcgcc tcccgtggga tggcttccac gccagattca
     4201 cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca ttgcttcaac cgtcggcgtt
     4261 aagccgatgt gtggctccag cgattccgga attacgccga ccagggtcag ctttttcgga
     4321 aactcgccgg tgaagcgcag ggccgacaag acgtcggcca ggccaagctg atgcggagag
     4381 attttgttgg taaacaacgc cggaacttct tcatcccgca ggatcatcat cgttcccggc
     4441 gcgctcttct tcgacacaat ggcatccgca ataatcagat gatcgcgatt tgccatgtcg
     4501 ccaagcagct ccattcccgc cgtgccgcca tcgaggatct caacataatc cggcagaatg
     4561 tatcgttgct ctaacgcttc gacaatccgc acaccgatgg cttcatcggt cagcaaaata
     4621 ttgccgaccc ctaagactaa aatacgcatt acagaacctt cactgaaacc acttcgttgc
     4681 cgtcagcatc cactacgtgt accgcacagg ccatgcacgg gtcgaaggag tgaatggtac
     4741 gcaccacttc cagcggttta ttcggatcag caaccggtgt acccaccagc gactgctcgt
     4801 aaggaccgac gtcatcattg aagttacgcg ggccagagtt ccaggttgat ggaacaactg
     4861 cctggtagtt gctgatgata ccgtctttga tcaccatcca gtgagagagc ataccgcgcg
     4921 gcgcttcgag gaagccaaca cctttgaact cacccgttgc cggaatgttc ggcttcacaa
     4981 aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc actgtattgg ttttgcagga
     5041 tatcctgcaa ttcacagcag tgaacggtac gaccaataat acggcccagc gtggagtgca
     5101 gctgtgccac ttccagcgtg ttgccagtca gtttctgata aatcgcaacg atttcattca
     5161 gtttgttctg ggtagattcg cgacctgccg ccagtttcac cagcatatta gccagcggac
     5221 ccacttctac cgttttgccg tagaaagtcg gtgatttcac ccaggaatat ttcccgtcgt
     5281 cagaccaacc atcataagcc ggaatggtgg taccttccca cggtgcctgc ggcgcttcgt
     5341 ctttatacca ggagtgcttc gcgctttcct gaatgccttt gatcaggtat tcatcggaat
     5401 gagaagtgat cggacgatac gaggacagat ccgcattctc aatgtagccg cccgggaaca
     5461 ggaagctgcc gtttttactg tcggtcggga attccggcac gctcaggtag ttcaccgcac
     5521 ctttaccgcg cgtcagccat tccgggtaga acgcagcgat aaccgcagta tcgaccttgt
     5581 aaacctgctc aacaaagtca ctcagcttgt cgatgaaaga cttgatgtac atcaggcgct
     5641 caaggttcag cacgcccaga ccgtcgaggt tgattgggtt agcgacccca cctaccgcca
     5701 ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac gacgcggtta gcgtcacgct
     5761 ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag gttcacttcc ggcggcagtt
     5821 tcatcgccgg gtgaccccag tagccattgg cgaaaatacc caactgaccg ctggcaacca
     5881 gatctttgat cttgttctga actttggtga actcttccgg actgttcagg tgccaggtcg
     5941 aaacaccttt cagcatttcg gaggctttgg ttgggtcagc ttgcagtgca gaagtgatgt
     6001 ccacccagtc cagcgccgaa agctgataga aatgaacgat atggtcatgc gtggtgtgcg
     6061 cagccagaat gatgttacgg atgtattgcg cgttaaccgg aacgtcgata ttcagcgcac
     6121 tttctgccgc acgaacggaa gacagcgcgt gagtggtagt acatacgcca cagatacgtt
     6181 gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac gatctcttcc atgccgcgcc
     6241 acatggtacc ggaagcccat gctttcgaaa cgacgccatt ttcgatttcg caatcgatgc
     6301 gtaaatgccc ctcaatacgg gttaccggat caatagtaat tctctggctc atgctttgct
     6361 cgcctcatga cgattatgat cgttttgttt taaaggagga agtatcggca gtagacgaat
     6421 gagtacgatg taagcgcaaa tctcaatagc cacaaaacca atagaaatca acagttcttc
     6481 ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat gccaccagcg aataggtcag
     6541 acgccaggtt gcacaaccta acagcgcgct cagtgctgac aggaacagca tgcgggaatc
     6601 attacgcagc ttcgccacac gcagaacgac cagcgggaag agcatcagca ggacttcaat
     6661 ccagaacatc acggagtaga agtcaccggc aaacgctaac gacagcttgt cgcgatagat
     6721 aagctcgcca aagcgcagca cgatgaaaat cgccagcaac acactgatgg tgttggtcag
     6781 cttgacgaat aggctctttt catccggacc gttgccacgc agacccgcct gcaccagtga
     6841 accttcaaag atgacaatcg agaagcccat gatgaacgcc gtcagcagcg agaacagcgg
     6901 caacatttca tagctctgcc acagcggatg caccttgtag cccgccgaga tcatcagcga
     6961 ccccattgaa gactggtgca tggtcggcag cagcgcaccg agcgcgatga tgaagaacat
     7021 caccttgttc agatgctgta gcgacacctt ccagcccaga cgttcaaaca gtgccggagc
     7081 aaactccaga gccatcacgc cgatgtagat ggtcatacag accgccgtct cgaacagtac
     7141 cgagttcacg ttgaagtgac ccggaatgta gaagtacggc aggttccagt agcgacccac
     7201 gtcgatagtg atcgacaagc cacccagtga gtaaccaaac agactcgcca acagcgccgg
     7261 acgcaccagc ggatggtatt gcccacggtt aaagacgtat accgcccacg caagcgccca
     7321 gccgccacag gcaaagccgg tgccaatcag caggtcaaac gcgatccaca cgccccacgg
     7381 gaagccgccg ttcaggtcag agacagagcc cagaccgaac accagacgct tcacaatcag
     7441 gagcatacag atgacgatta acggtccaaa aatcatgacc ggtttactga tgattttgcc
     7501 gcccagcggt tgtggatcat gactcatgat cgtctcctcc gtcgtgatgg tcgtttttgg
     7561 tgttgcgacg aaccagcacg gtcaagcccg ccagcacagc cagtggtagc atcatgcctt
     7621 tatacagggt gtgttgaata ttttcggaac gcgcaccggt agaaagatcg tccagtttcg
     7681 gcaggtcgag attttcataa ggcacacccg tcagtaccag aacctgagta ccgccgccct
     7741 ctttctcgcc gtacagatgc ggataatatt tcggcaccgt atgcaggtaa gtgtcgccag
     7801 atttcagcgt ctgacgcgga tagtggtatt cgctgccagg cttcagcgcc agacgttttt
     7861 tcgcctccgc catcagctct tcacgcgtac caaaaatcac cgcgcccgcc gggcacactt
     7921 ctacgcagcc aggcagaccg cctttatcga gacgttccac acctttctgg ttgcacagct
     7981 cgcacttatg cagcgcacca aacgggttgt tgtagtcgta cttcggcacg ttgtacggac
     8041 aggcgaccat gcagtaacgg caaccggtgc acacgtcttt gtcgtaatgg acaatgccgg
     8101 ttttcggatc ttttttcagt gcagagaccg ggcacacaga gacacagttc ggatcgacgc
     8161 agtgcataca ctgtttctta atgtacgcgt agccgttctc ctcctggtct ttgttgaccc
     8221 ctgtgccgct ggtccacact tgaatgatgt tattggtata cggcgacagt ttgtcgttgt
     8281 tcgaccaggt ctgttcccct tgcgggttac gttcagggaa gttgatatcc tgacacttgg
     8341 tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta caacatcccc agcgatcccg
     8401 gaattggcgg gcggttttca gcagccgcat gactgacaga cggcagcgcg cccgtcagca
     8461 atgccccgca ggaggctgct ttaataaaat tacgtctgtt cacggttatt ctccccgtga
     8521 gtcagcgtta tctttctttt gctgacgacc cagttcacgc accgccatca cgctgacacc
     8581 ggcaaccagc ccaaccacac cgccgagcaa accaatagcg cctgcagaga cgttgccgcc
     8641 ctctttagcg ttaacatccg gtttctgtga acgcggagtc tgattttcga cgttggcaag
     8701 ctgatggatg cctttatgga agccgatacc ttcttcgtta cagccatagc acgggtgacc
     8761 aatcgccacc ggccacacac cgccaacatc gcagaattgc agcgttgagc agttgccgta
     8821 agtttctggc cctttacagc cgaggtggta caggcaccag ccttcgcggt ggccttcatc
     8881 accgaactct ttggcaaaac gaccagcatc gaagtgcggg cgacgttcgc agtgttcgtg
     8941 aatcagacgg ccataggcga aggtcggacg gtttttgtca tccagtttcg gcggtttgcc
     9001 gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc gggttcggcg ggcagcccgg
     9061 aatattgata acggttttgc ctggcagaac ttcttgcaag ctgactgcgc cagttgggtt
     9121 aactccagct gcggcaacac cgccccacgc agagcaggaa ccgatagcga taatggctgc
     9181 tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc tcaccggcaa ccatgcaata
     9241 aataccgtta tcttttaatg ggatggaacc atccaccact aacacatact gccctttgta
     9301 cttctcgaga gcgttatgtt tgttctcttc gacctgatga ccgaaggcgg cggaaagcac
     9361 ttcgtgatac tccagagaga tagtctccag tacgaggttt tctaccgttg gatgcgttgc
     9421 acgaagcaga gattccgtac aaccggtgca ctcctgcgcg ccaatccaga taactggcgg
     9481 acgctgcggg ttagtaaccg attcggccat ctctgcagcg gctttgctac ttaaccccat
     9541 ggtggcggct aatgctgcac aaagcttcat gaaatcacga cggttaatgc cgtgagaatg
     9601 gatgagggtg ttatctccag tcatttatag ttattccgtt gcgaagacct ggcatatatt
     9661 ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca tttaccacac atttattatg
     9721 gttatcgata tcatgatact gcggcggggc gctaaaacga agggaagaag cattagtgta
     9781 attaattaaa gcaagataat acgtatgttt gatcaatttt cgaggcgata accggccata
     9841 aaaaagcgca acgtagaaca ggaattattc tcacgaaaga aatccattac catctcttta
     9901 tccagtccat agcggcgcgt cagaataccc gcttcccagg cgctaagctg gcgatctccg
     9961 gtcttttcgt acatccggtg tgagtagcct aatacaaaac cgcgtttata atcggcacag
    10021 aattttgtca cattaaccgc actgtcggca tgcgttgctt ttaatcccgc cattaagcct
    10081 ttaccaaaat ggttattcat cgttcacctc attcgctata tatcgtaata tatagcgata
    10141 ttttaggcaa agaacgactt ttgcgcacgc tattttttca tgcttgttta agacgtaatc
    10201 ccgtaagaga taaacctatc aggagagata tttatcgaac catttcagtg tcctttgcca
    10261 ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa tcattatgga atccgtgatt
    10321 aacccccgga tagatatacg cctcataaac cttattattg gctttcaacg ccgcctcgta
    10381 agcaggccag ccctcgttga ttcgggtgtc cagttccgcg aagtggagta gtaaaggcgc
    10441 ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca taaaacggca ctgcgcaggc
    10501 cagttccggg taagcgaccg ctgccgcgtt cgatacgcca ccgccatagc aaaatccggt
    10561 aatacccact ttgcctgtcg cttgcggata gcgttgcata aactcaattg cggcaaagaa
    10621 atcattcatc agtttggttg gatcaacctg ctgttgcagc tcacgacctt tatcatcatt
    10681 tcccggataa cctccaacgg aatttaagcc gtcaggtgcc agggcgatat accccgcctt
    10741 cgccactcgc cgtgccacat cttcgatata cggattcagt ccacgattct catgcaccac
    10801 caccacggct ggcgttttgc cgctcatttt tgcgggttta accagataac cccgtacctc
    10861 gccgtgacca tttggcgaag gatacgtgat gtactcagca aaaatctccg ggtcggtaaa
    10921 ctctacctga gtcgccagcg cataatttgg cttgagcaaa tcaaacaacg ctaatgccgt
    10981 catcccgcct accgcatact tcgccgcaag gttgaggaac tcacgtttcg agattttccc
    11041 gtgagcgtaa tagtcgtagt aatccagcaa ctcttgtggg aaatctttgg cggttaaacg
    11101 cggcatcgtt gcactcctca gttggtgttt ttttaagcaa agcataagca cgtgtttttg
    11161 cccagttttt cgtcactctg tgagccagac tacgggatac gcgctggcga atcgctaaac
    11221 tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg tcatggtctg gttagcgaat
    11281 cccgaacgtt acgggcagat gcaataccgc tattgcggaa aaagcggttt acgcctgccc
    11341 gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta acgcgctgga atcacagcgt
    11401 gcgatcctgc gtaaagcgtt tgatttaggc attacgcact ttgatttagc caacaattac
    11461 gggccgcctc caggaagcgc agaagagaac tttggtcgcc tgctgcggga ggattttgcc
    11521 gcttatcgcg atgaactgat tatctctacc aaagctggct acgatatgtg gcccggccct
    11581 tacggctctg gcggttcacg taaatacctg ctcgccagcc tcgaccaaag cctgaagcgt
    11641 atggggcttg agtatgtcga tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg
    11701 gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg
    11761 atctcctctt actcaccaga gcggacgcaa aaaatggtcg agttgctgca cgagtggaaa
    11821 attccgctgt taattcatca accttcgtac aatttactaa accgctgggt ggataaaagc
    11881 ggcctgctgg ataccctgca aaataacggc gtgggctgca ttgcctttac tcctctggct
    11941 cagggattgc tgaccggaaa atatctcaac ggcattcctg aagattcacg gatgcatcgt
    12001 gaagggaata aagttcgtgg tctgacgccg aaaatgctca ccgaagccaa cctcaacagc
    12061 ctacgcttat tgaatgaaat ggcacagcag cgtggacaat caatggcgca aatggcgtta
    12121 agctggttgc tgaaagatga gcgagtgacg tcggtattgg ttggtgccag ccgcgcggag
    12181 caacttgagg agaacgtgca ggcgctgaat aatctgacat ttagcaccga ggagctggcg
    12241 cagatcgatc agcatatcgc cgatggcgag ctgaatctgt ggcaggcgtc ttccgataaa
    12301 tgacctgtta ataacgggcg cgagaaccgc gcccgaatca gatcagtgat tatgacgagt
    12361 cagtcggtca agatagccca ttacaaatgc agagagcaca aacgtcagat ggataatgac
    12421 gtaccacatc agtttgttat cagggacatt tttcgcatcc ataaagacgc gcagtaagtg
    12481 aatggaagaa attgccacaa tcgacgctgc tactttgttt ttcagcgacg ttgcatccat
    12541 tttccccagc cagttcagct tctctttgtt ctcggagata tcgagttgtg agacgaaatt
    12601 ctcataaccg gaaaacatca ccatcaccag caaaccgcca accagcgtca tatccaccag
    12661 cgacagcaac acgaggatca aatctgattc cgccatcgag aagatattcg gcagtacgtg
    12721 aataatctcc tggaagaact tcagcgccag ggcaactaac gccagcgaaa ggccaaagta
    12781 cacgggggca agcagccagc gagaagcata cattgcattt tcaagaaaac gttccataca
    12841 ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa ttgagtaaac agcgcgcaac
    12901 agcaaatcaa catatccgtt aaatctttgt cgcataattc tgagggttag ggatcttcct
    12961 ggttccctta tcaacgcatc gagagcgtct acccttaaag gacgttgaac ctgaagggag
    13021 aaaaacgatg tctcatttaa aagacccgac cacgcagtat tacactggtg aatatcccaa
    13081 acagaaacaa ccgacgccag gcatccaggc gaagatgaca ccggtaccgg attgcggcga
    13141 gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa gccctggtga cagggggcga
    13201 ttccggaatc ggtcgcgctg ccgccatcgc ttacgcgcgt gaaggggctg acgtggcgat
    13261 cagttatctt cccgtggaag aagaagacgc tcaggatgtg aaaaagatca ttgaagaatg
    13321 cggacgcaaa gccgttctgc tgccaggcga tttaagcgat gagaaatttg cccgttcgct
    13381 ggttcacgaa gcgcataagg ccttaggtgg gctggatatt atggcgctgg tcgccgggaa
    13441 acaggttgcc attcctgata ttgcagacct caccagcgaa cagtttcaaa agacctttgc
    13501 cattaacgtt ttcgcgctgt tctggctaac ccaggaagcg atccccctgc taccgaaagg
    13561 tgcaagtatt atcaccactt cgtcaatcca ggcataccag ccaagcccgc atttactgga
    13621 ctatgcagct acgaaggcgg cgattctgaa ctacagccga ggcctggcaa aacaggtcgc
    13681 ggagaaaggt attcgggtga atattgtcgc gccaggcccg atctggacag cactgcaaat
    13741 ttccggcgga caaacgcagg ataagatccc gcagtttggt cagcaaacgc cgatgaaacg
    13801 tgcggggcaa ccggcggaac tggcccctgt atatgtttat ctggcaagtc aggagtcgag
    13861 ctacgtcacc gcagaagtgc acggcgtgtg cggcggcgag catttaggtt aaaaaaatgc
    13921 ccggttgtga aaagcaaccg ggcatcattg tgaattcact cttacctgat gtggcgaaaa
    13981 cgccttatcc gcctacaaat gcatgaagat tcaacatgtt gcaatagatt ttgtaggcct
    14041 gataagcgta gcgcatcagg cattgagcac ctaatgccgg atgcggcgtg aacgccttat
    14101 ccggcctaca aacgcacgaa gattcaacat gttgcaatag attttgtagg cctgatgaac
    14161 gtagcgcatc aggcaattct actttacttc gctttggcgg tttcttcgcc gaccagacct
    14221 atcttcaggt aacccgcctg atgcagcgtg tccattacct tcatcaacgt ctcgtaatcg
    14281 acagttttat ccgctcggaa gaagatggtg gtgtctttct tgccttcggt taacgcattc
    14341 aacgccgtaa tcattgtttc atcggtgacc ggatcgttac cgataaacat cgagttgtct
    14401 gccttcaccg acagataaac cggtttttcc gggcgcggct gcggcgtgct ggtagaagca
    14461 ggcaagttaa ccttcacatc taccgtcgct aacggtgccg ccaccataaa gataatcagc
    14521 aggaccaaca tcacgtcgat aaacggcgtc acgttgatat catgcatttc gccgttatcg
    14581 tcgaggtttt cgttaagatg cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg
    14641 acacgaaccg gatgcgcagc ggcgctggct tccagatcca ggtcacggct ttgcagcaac
    14701 aatacctgcg ctgcgacatc acccagcatc gctttaaagc cgccaatctg acgcgcgaat
    14761 acgttataga taacgaccgc cggaatcgct gccaccagac cgattgccgt tgctaacaga
    14821 gcttctgcga tacccggcgc aacgactgcc aggttagtgg tctgcgtttg cgcgatacca
    14881 ataaagctgt tcatgatgcc ccagaccgta ccaaacagcc caacgaacgg cgaaatcgcg
    14941 ccgatggttg ccagatagcc gttaccgcgg cccatttgac gacctactgc ggcgacccga
    15001 cgctccagac ggaagctggt acgttcttta ataccttcgt tatcgtcgct gccttctgac
    15061 agttccagct cattctgcgc ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa
    15121 tcagcggcga tatcgttggc ctggtttaag gaacgcgctt cagccagcag ttgctgctcg
    15181 cgcttaaggc gacgcttctg attaaagaac tctacgctct tactgaagaa gattgcccag
    15241 gtgactacgg aggccaaaat aagcccaatc atcacgcact taacgacaat atcggcgtgc
    15301 tgatacatac cccagacgga aaggtccgtc tgcattaaat tattacccac tgtgtatctc
    15361 caggacgcaa gtcacaaaat ctgcgcataa taatatcaaa acgacgtcga attgatagtc
    15421 gttctcatta ctatttgcat actgccgtac ctttgctttc ttttccttgc gtttacgcag
    15481 taaaaaagtc accagcacgc catttgcgaa aattttctgc tttatgccaa ttcttcagga
    15541 tgcgcccgcg aatattcatg ctagtttaga catccagacg tataaaaaca ggaatcccga
    15601 catggcggac aaaaagcttg atactcaact ggtgaatgca ggacgcagca aaaaatacac
    15661 tctcggcgcg gtaaatagcg tgattcagcg cgcttcttcg ctggtatttg agagtatgga
    15721 agccaaaaag cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg
    15781 aacgttaacc catttttcct tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg
    15841 cgcgctattt ccctgcgggg cggcagcggt tgctaattcg attcttgctt ttgtcgaaca
    15901 gggcgatcat gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa
    15961 aatcctcagc aaactgggcg taacgacatc gtggtttgat ccgctgattg gtgccgatat
    16021 cgttaagcat ctgcagccaa acactaaaat cgtgtttctg gaatcgccgg gttccatcac
    16081 tatggaagtc cacgacgttc cggcgattgt tgccgccgtg cgcagtgtgg tgccggatgc
    16141 catcattatg atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc tggattttgg
    16201 catcgatgtt tctattcaag ccgccaccaa atatctggtt gggcattcag atgcgatgat
    16261 tggcactgcc gtgtgcaatg cccgttgctg ggagcagcta cgggaaaatg cctatctgat
    16321 gggccagatg gtcgatgccg ataccgccta tataaccagc cgtggcctgc gcacattagg
    16381 tgtgcgtttg cgtcaacatc atgaaagcag tctgaaagtg gccgaatggc tggcagaaca
    16441 tccgcaagtt gcgcgagtta accaccctgc cctgcctggc agtaaaggac acgaattctg
    16501 gaaacgagac tttacaggca gcagcgggct attttccttt gtgcttaaga aaaaactcaa
    16561 tgatgaagag ctggcgaact atctggataa cttcagttta ttcagcatgg cctactcgtg
    16621 gggcgggtat gaatcgttga tcctggcaaa tcaaccagaa catatcgcag ccattcgccc
    16681 acaaggcgag atcgatttta gcgggacctt gattcgcctg catattggtc tggaagatgt
    16741 cgacgatctg attgccgatc tggacgccgg ttttgcgcgg attgtataac attgccactt
    16801 ttggacaatt ttgcagacat tttattgtga aaagtcttaa attgttgcgt ccgggatcaa
    16861 ggcgtcccgg acgattcagg agtacaatag gcagataaag gcttaaacgc tgttccacag
    16921 gaaagtccat ggctgttatt caagatatca tcgctgcgct ctggcaacac gactttgccg
    16981 cgctggcgga tcctcatatt gttagcgttg tttactttgt catgtttgcc acgctgtttt
    17041 tagaaaacgg cctgctgccc gcctcatttt tgccaggcga cagcttgttg atattggctg
    17101 gcgcattgat tgcccagggg gttatggatt ttctgcctac gattgcgatt ctgaccgccg
    17161 cagcaagtct gggctgctgg ctaagttata ttcaggggcg ctggttaggg aataccaaaa
    17221 cggtgaaagg ctggctggca cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt
    17281 ttgaccgcca cggtctgctg gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc
    17341 tgctgccaac catggcggga atttccggtc tgccaaaccg ccgtttccag tttttcaact
    17401 ggttaagcgg attgctgtgg gtcagcgtgg taaccagttt cggctatgcc ttaagtatga
    17461 ttccgttcgt taaacgccat gaagatcagg taatgacgtt cctgatgatc ctgccaattg
    17521 ccttgttaac cgctggcttg ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta
    17581 acgcctgacg attttccctg ttcccggttg ctgtaccggg aacgtattta attcccctgc
    17641 atcgcccgca ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg tttaaactca
    17701 cggctaaatt gcgatgcgct ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg
    17761 ccgtcatgga tgatcatcat ccgcgcctta tgcagacggt aattcttcaa atactgcaac
    17821 ggcgaggtgc tggtgacaga cttaaaatta tggtggaacg ccgatacgct catattagct
    17881 tctgccgcca gttgctcgac gctcaggttt tcggtgtatt tattctcaat ccgtttcagc
    17941 acgcggctaa tcagactgaa gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg
    18001 caaggtccgg tcagcacgta gtacagaatt tcgcggatga tctgtttgcc gagaatacgc
    18061 gcatccagtg gtcgcgccat cacgtcgagt aaccgctccg ccgcgcataa aatctcttct
    18121 gataacgtgg cggagttaat cccgctggct gccatcgacg gctggaaatg ctcatcttcg
    18181 ccaatatcca tcaacagttc ctgtaactgc aaaatatcga cattgagacg caaccctgcc
    18241 agcggcacct ctgacgtggc ataggtttcg cactcaaacg gcaacggcac cgtcagcagc
    18301 aggtattcat tggcatcata acgaaacacg cgttcattga tataaccgat tttatgtccg
    18361 gaaaagagaa ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa aggcgtctcg
    18421 ccatacaaca aacgcacatc gggcaacagt tctgacaaac tattttcttt atttttcagt
    18481 ttattaactt tatccgccag caagcggcaa atctcttcac gtttcatatc gcgtaatttc
    18541 ttaggaataa tgcggcaatt tgattgtgcg caattttgta gcatttctcc agcactctgg
    18601 agaaataggc aagacattgg cagaaatgag cattgagagc cagggcgctg gcgatcacaa
    18661 tgaaaaacat caggcagatc gttctctgcc ctcatattgg cccagcaaag ggagcaagta
    18721 atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct
    18781 ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg tggcggcagc
    18841 gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg
    18901 gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg
    18961 gttcgcgaac agaaagtgac tttcctgctg ggggttggcg gcggttctgt actggacggc
    19021 accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg
    19081 caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca
    19141 gcaaccggtt cagaatccaa cgccggtgcg gtgatctccc gtaaaaccac aggcgataag
    19201 caggcgttcc attcagccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc
    19261 tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg
    19321 gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt
    19381 ttgctgacgc tgatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg
    19441 cgcgccaacg tcatgtgggc ggcgacgcag gcgctgaacg gtttgattgg cgctggcgta
    19501 ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat
    19561 cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag
    19621 cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgacgat
    19681 gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgcct
    19741 acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg
    19801 gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgctgga tgtcagccgc
    19861 cgtatatacg aagccgcccg ctaagctttt tacgcctcaa actttcgttt tcgggcattt
    19921 cgtccagact taagttcaca acacctcacc ggagcctgct ccggtgagtt catataaagg
    19981 aggaacgtat ggctaatcca accgttatta agctacagga tggcaatgtc atgccccagt
    20041 tgggactggg cgtctggcaa gcaagtaatg aggaagtaat caccgccatt caaaaagcgt
    20101 tagaagtggg ttatcgctcg tttgataccg ccgcggccta taagaatgaa gaaggtgtcg
    20161 gcaaagccct gaaaaatgcc tcagtcaaca gagaagaact gttcatcacc actaagctgt
    20221 ggaacgacga ccacaatcgc ccccgcgaag ccctgctcga cagcctgaaa aaactccagc
    20281 ttgattatat cgacctctac ttaatgcact ggcccgttcc cgctatcgac cattatgtcg
    20341 aagcatggaa aggcatgatc gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt
    20401 gcaacttcca gatccatcac ctgcaacgcc tgattgatga aactggcgtg acgcctgtga
    20461 taaaccagat cgaacttcat ccgctgatgc aacaacgcca gctacacgcc tggaacgcga
    20521 cacacaaaat ccagaccgaa tcctggagcc cattagcgca aggagggaaa ggcgttttcg
    20581 atcagaaagt cattcgcgat ctggcagata aatacggcaa aaccccggcg cagattgtta
    20641 tccgctggca tctggatagc ggcctggtgg tgatcccgaa atcggtcaca ccttcacgta
    20701 ttgccgaaaa ctttgatgtc tgggatttcc gtctcgacaa agacgaactc ggcgaaattg
    20761 caaaactcga tcagggcaag cgtctcggtc ccgatcctga ccagttcggc ggctaacgtg
    20821 caaattctcc cggtggcggt aatgttccgc taccggactt ttcagaaatc atttattccc
    20881 ctcgcgtccc gcccgttgtt actcttcctt gttcaggaat gccaaatata aggacatcat
    20941 catgcagagc cggaagctct taaaagaaca actcatctat atccgggata aacgcaacgg
    21001 agaggtgaaa aacagatgaa aataatactt ctgtttttag cagccctggc aagttttacc
    21061 gtgcacgcac agcccccctc acagaccgta gaacaaacag tccggcatat ttatcagaac
    21121 tataaatcag atgccactgc cccttatttt ggtgaaaccg gagagcgtgc gataacttct
    21181 gtgcgtattc aacaggcgct taccctgaac gacaatctta cgctgccggg taatattggc
    21241 tggattatga tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgtag
    21301 cgataaccga aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag
    21361 atgataaaga aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca
    21421 ttgacgatat tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa
    21481 aaacgctggc cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac
    21541 tctttgaaca tattgccgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc
    21601 gccaggcggt caatgccttc tataaaacca ccttcaagac ggccaacaat cccgatgaag
    21661 atatgcaaat agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc
    21721 tattttcacg cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc
    21781 atattcgttt tacgctgacc aatggcaaca acgaagagca agaactggtt ttacaacggc
    21841 gcgaaggtaa gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc
    21901 agattgaggc aaaaaccgcc gccagattaa agcaatgagc tgaattaaat agcaattagc
    21961 cggaacaata aaaaagggaa cactatatga aaacgatttt caccgtggga gctgttgttc
    22021 tggcaacctg cgtgctcagt ggctgcgtca atgagcaaaa ggtcaatcag ctggcgagca
    22081 atgtgcaaac attaaatgcc aaaatcgccc ggcttgagca ggatatgaaa gcactacgcc
    22141 cacaaatcta tgctgccaaa tccgaagcta acagagccaa tacgcgtctt gatgcacagg
    22201 actattttga ttgcctacgc tgcttgcgta tgtacgcaga atgataaaaa aatccccggc
    22261 agcatgtcag ttgccgggga ttttttttaa cgtccaaccg ccgctttagg gcgtttcttc
    22321 gcaccagcat tcaccggacg agattgcgta gacgacgctt tttttgccgt agcaggcgtc
    22381 tgacactggg tcgccatcgg cgtatgtttc gtcaacgccg gacgggtatt gcggttctgg
    22441 cgacgagcct cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg
    22501 ccaatcagat gctttttgcc catcgcttcc agcgcctggc ggattaacgg ccagtttgcc
    22561 ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct gtttgtcgcc cttcggtacg
    22621 aagacgtctt cactcttata accaatcttc gccagcgggt ttttcccggt gtaatacatg
    22681 gtggtcgagt tagccagtgg cgatgggtag aagttctgta cctggtcgag acggaaacga
    22741 tgctttttca gccacagcgc caaattcacc atatcttcat cacgcgtacc ggggtgcgcg
    22801 gagatgaaat aggggatcag atactgctct ttacctgcct gtttcgagta ggtatcgaac
    22861 agctctttaa agcggtcata actgcccatg cccggcttca tcatcttcga caacggccct
    22921 tcttcggtat gttccggagc aatcttcaga taaccgccga cgtgatgggt cgccagttct
    22981 ttgatatagc gcggatcttc cacggcgatg tcataacgta cgcctgaggc aatcagaatc
    23041 tttttaatgc ctttcagatc gcgagcgcgg cgatagaggt tgatcgtcgg ttcgtggtta
    23101 gtgtccatat gcggacaaat atccgggtaa acgcaggaca aacgacggca ggtttgttca
    23161 gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc gagatcggaa
    23221 attacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa
    23281 tcttcggaac ggctctgaat aatgcgccct tcgtgctcgg tgatagaaca gaaagagcag
    23341 ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga tcatttcgta agccggaata
    23401 cgggcattgc cataggccgg atgtggcacg cgcttgtacg gcagcgcaaa aacgctgtcc
    23461 atctcttcgg tagaaagcgg gatagcaggc gggttgatcc acacatagcg gtcaccgtgt
    23521 ttttgcatca atgcgcgggc gcaacctggg ttagtttcgt ggtgcagaat acgcgaagca
    23581 tgggcgtaca gcactttatc gcccttcact ttctcgaaag aaggcagcaa cacgtaggtt
    23641 ttttcccacg gtttcgggcg cggtggctgc acggttacgg ctttggcttc ctgctttttc
    23701 ggtgccaccg gtttgttatc cgcgcacggc aaatcttcac catacggatg cgggattggg
    23761 tcgatttttc caggggtatc aagacgggtg gaatccacgc cgctccagcc aggcagcgcc
    23821 tctttcacga taatcgcggt attacgcaca tcgcggattt cactaatcgg ctcgcccatc
    23881 gccagacggt gcgccacctc caccagcgga cgctcaccgt taccaaacat cagcatgtcg
    23941 gctttcgaat ccaccagcac ggaacggcgc acggtatcgg accagtaatc ataatgcgcg
    24001 gtacggcgca gactagcctc aataccaccg aggatcaccg gtacatcttt ccacgcctct
    24061 ttacaacgct gggtataaac cagtgtggcg cgatccgggc gcttacccgc gacgttatcc
    24121 ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg tataacggtt gatcatcgaa
    24181 tccatgttgc cagcagtaac accgaaaaac agattcggtt tacccagacg catgaaatcg
    24241 tctttgctgc tccagtctgg ctgggcgatg atcccgacgc gaaagccctg tgcttccagc
    24301 atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc gccagtaacc
    24361 aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga catcggcaaa
    24421 aacggtgccg gtccaaaaca ggcggcccag tactgcggcc aggagaacag gtcgcgatcc
    24481 ggttggatca gggagataga gctcattttg cttccagaaa tgataaaaaa ataatcaaag
    24541 gccggggatt ataagccgga acgaaagaga aatcgaaaag tattccatac tcgccctcct
    24601 cgggcgagta tgaagattac ggtaccggat tgactaacag ttgcccaatc gagccacggt
    24661 ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac
    24721 cgaaataaac aagcagctcc acttgtccgt caacccaaac ggtatctttc cagcctctgt
    24781 cttccgggaa cggcatcgca ccattcacgt tacggatctg gaacatcacg ccttcaatat
    24841 ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg
    24901 tgacatcaat acggttgacg tcccacagtt gtccattaat acccgggtca tcacccagac
    24961 tgatatcgcg actgcgaatc ggcgaaccgg ccatgatttc agttggcagc aagcgcatcg
    25021 gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc accagggtag
    25081 aaaccagaat gctggatggc tcaaagaagc cacgaatacg atcaacaatg ctcgccgctt
    25141 cgccacaggt gatcgacact tcatcaccgt tgctcatatc caccagaatc tcgcggcgct
    25201 cgcccggtgc caacgaaagt tgcttcaccg atacaggagc aggcaggaat ccctgatcgc
    25261 cagaaatcac atgtaacggg cgaccatcgc tcatctgtag ttgatagcga cgagagttcg
    25321 acgcgttcag cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac gggctttgta
    25381 caccgttaac cagcagcgta tcaccaacaa agccgccgct tcccggttcg ttgtattctg
    25441 gcgtaccaaa gttatccagc cgtttatcct ggataatgac cggaaaatca tccacaccat
    25501 aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac attccggcaa
    25561 ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac cacagagtag
    25621 ctgcgttctg gcgaataggc agtacgggtg cccagtcagc gtttggcgac atcatccgtg
    25681 ccggaccgcc catcagcggg cctggtacct gtagcccggc caccgtcatt gagacatttt
    25741 ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg cggatagtcg
    25801 gccccaggta acgaccattg attccccaga ccgacgcgcg tgtccctggc gtaaatgacc
    25861 agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca agcagcggcg
    25921 gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca gcgcctgcac
    25981 aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata tttgctccat
    26041 gtaaaactgg ctaaagtaat accgtgcagg aaagtgtttc ccgcaaaaat acagattgcc
    26101 gacttaaact tttccggcgg cttcgcgctc tgcgacttct ttatcgagct cggcgatttt
    26161 ttgttccatt atcgaacgac aatgggcagc cagctcacga acctgatctt tgccatactg
    26221 actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgca gtcgattaag
    26281 attaatctta ttcgaagttg tggagacgca cacgggaata atcgggacgc ccgccgcaat
    26341 tgccgcgtga aatgccccag tcttgaacgg tagcaggccg cgaccacggc tgcgggtacc
    26401 ttccgggaac atccagatgg aaatacggcg ttttttgaaa tgattcacta cttccgcaat
    26461 agtgccgtga gctttagtgc gattgtttct gtcgatcaat aagttgccgg ttaaccagta
    26521 caactgcccg aagaagggga tccacagcaa gctcttttta cctaccgtca ccgtcggcgg
    26581 ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag cgatatagat
    26641 agcattgccg tagctttcag catctgcagg tttgcggcac tcaactttca ggccgaacag
    26701 cggcgcaaga cgaccaaaca tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct
    26761 gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca cggtaataat
    26821 aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc attgtacgtc
    26881 acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc
    26941 gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg ggttgaatac
    27001 gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacagcg
    27061 ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc
    27121 tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca
    27181 gtgactttct gtagctcttc cggacgcagt ttaattttgc gtttcccaac atgaatggtc
    27241 agcgtgcttt gcggcggcag aacgtacagt tgcgccagac catcctcgcc acgcgcggct
    27301 tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatca
    27361 cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca
    27421 tcttcaatta ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcctta
    27481 cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg
    27541 gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgcccca
    27601 ggcggcaacg ttaatttgcc ggtgagcggt tcgccctgac cacgcgccga cggcagcgta
    27661 atcggatcaa tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc
    27721 ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc cggcgcgtcg
    27781 atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt
    27841 tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc
    27901 gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta
    27961 ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagctca
    28021 ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg
    28081 aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt
    28141 tcatcttcat tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc caccagcaaa
    28201 ccttcgagga tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc
    28261 acggtatcgc ggcggaacac cagccattcg gagagaattt ccagcaggtt tttcaccgcc
    28321 ggacgaccat ccagaccgat catattaagg ttaatgcgat agctcttttc cagatcggtg
    28381 gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc
    28441 accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc
    28501 tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc
    28561 ggcaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact
    28621 gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat aatttccgct
    28681 tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc
    28741 tggtcgatta atgcgattgc cgcctgagcc acttcacgca ggttatgcgg tggaatatcg
    28801 gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgggca
    28861 ggtagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc
    28921 tgccccaact cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc
    28981 attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc
    29041 ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca ggcgctatcg
    29101 ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg
    29161 gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg aacaggtttc
    29221 agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag
    29281 taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct
    29341 gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat
    29401 ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc
    29461 gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacataa tcctggggat
    29521 tatgcagcgg aaaaccgcca acgtagggtt taagcagttt gattaacggc tgatagtaga
    29581 taggaatcag cggtgcctgc tggttgacga tcacttctgc ctgctgatac aacgcattac
    29641 gctttgtcgc atcagtgatc tgcatggcct ggtttagtaa ggcgtcatac tgcgcatttt
    29701 tccagtgacc gacgttttct tcactatcgc ttttgagcgt gttcaggaag ctggaagcat
    29761 cattgtacgt cgcatcccac gactgccgag acagcatgaa atcaccggct cgtcgggcat
    29821 caagataggt tttccactcc attgtgcgca gcgtcacctg tgcacccagc cattttttcc
    29881 attcggaaga caacgctatc gcggtctttt catgcagatc gtacttgttg tagaacagct
    29941 caaagcgtag cggatgagag gcgtcgtatc ccgcctgttt cagcaaggct tttgccatcg
    30001 cgacgcgctc actcattggc ttttgcagtt catcgaacgt cgtcgcgcta aagcctttta
    30061 cctctggcgg cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt tgcgcaataa
    30121 gctgtcgatc aaccgtaaga tatagcgccc gacgcactcg cacatcgtta aatggcggtt
    30181 tctcaaggtt gaagttgtaa tattcgctgt tcagacgcgg aataattcgt atctcgccag
    30241 gcagtgattt ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga tcgacctctc
    30301 ccgcgcgata gcggttatag ccggtgaccg aattatccag cgccagatac tcaacctgtt
    30361 gcaatactgt atgttgcgca tcgcggtact ttggattttt gcgtgcagta atcttttcgt
    30421 taactaccca ctgatcaagc acaaaggcac cgttgtaaac catgttctct ggcttactcc
    30481 agctatcgcc atgtttagcg atgacatgat gaggaaccgg gaacagcgtc ggccaggcga
    30541 gcatcgtcgt gaaccaagga accggctgct caagcgtaac ttcaagagta cgatcatccg
    30601 tcgctttgac acccagcgat gtaacatctg ctttacccgc aacaatagcc gcggcattgt
    30661 taatgtgcgc ctgtgccaga tagccagcaa aagggcttgc cgttttcggg tcaaccgcgc
    30721 gctgccagcc gaggacaaaa tcctctgccg tcagaggctg accgtcggac cactgcaaac
    30781 cgctacgcag atggaaaata tagcgcctgc cgccgtccag tatctcccag cgttcagcct
    30841 gagcgggctg cacctggcct tcaccgtcca tccataccag accttcaaac agatccagca
    30901 caatctgcgc ggcagtattc tcctccacct tttgcgggtc gagcgtacct gggtcgctat
    30961 gattgttgta acgaaagact tgttgcgggg cgagctgtgt gttggtggga acgtcagcag
    31021 cgtagagagg agccgcactt accaggctga ccagccataa taaatttcgc gtatacatgg
    31081 tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat tgttcgctca
    31141 aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac gctaacggca
    31201 cataaacatt cgtttccagt cgctgctccg tcaccccttc ggcaagattg gtgtagtgaa
    31261 acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaataatgc
    31321 cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgagccacg gcataacgtc
    31381 caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc
    31441 tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg tcccaggcga
    31501 cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa
    31561 atttcgccac gctgtagttg agcaactcag gcctacagcg gtgcggcaac atcgccacag
    31621 ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatg ttgcctccct
    31681 gcgaaattat tcccgggaac gcatcccggg agcggtaaca attacggatt tactttgcgg
    31741 atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca
    31801 accgtatctt tcggcgtcac cgtcacgccg ttccagcgtt tgtggtcgat atcaacattg
    31861 atagtaccgc tggcatcttt gaacacgtag agatcgtcag agatgcgttc aacgatattg
    31921 ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg
    31981 ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg gccagaaaaa
    32041 ccgccctgct ctgctgccat caccggtgcg ctgcacaggg ccattactgc gattactgct
    32101 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
    32161 gtccttaaca acttcttaag ggaaaaaaat aaaatttagt gctgtacaga gcgcgttaca
    32221 acacggttta ctagcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
    32281 atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa aatgggtttt
    32341 agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat
    32401 gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa
    32461 tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga tgcgctggag
    32521 gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
    32581 atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca ggccagcaac
    32641 gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc
    32701 gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
    32761 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
    32821 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt
    32881 attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
    32941 ttagtctgcg tgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc
    33001 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc
    33061 aactgatgct gtttgccaag cggttaagta cgctcgatct caacgaaatc aacgcggcgg
    33121 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga
    33181 cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat aacggagaag
    33241 atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtg ggcgataaag
    33301 atcaatggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggtc
    33361 aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt
    33421 ggttggtcgc actgccggtt atgttaatca tcatgatggt actactgggt cgtgaactcg
    33481 cgccgctgaa caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac
    33541 taaacgcgac tggcgtaccc agcgaagtgc gaccactggt tgagtcgcta aatcaactgt
    33601 tcgcccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac gcagctcacg
    33661 aacttcgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg
    33721 atgatccgca ggcgcggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta
    33781 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    33841 acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat atttaccaca
    33901 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgtccagggc atcaaacgca
    33961 ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac gccgtgcgtt
    34021 acagtccaca gggcagcgtg gtggacgtca cgctgaatgc cgataatttc atcgtaaggg
    34081 ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    34141 cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca
    34201 agctgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaagg
    34261 taagctggta agggtaagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
    34321 ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa
    34381 atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
    34441 aacgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgttcgc
    34501 atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct
    34561 gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa
    34621 tacattgatg atgtattcac cgaaggtcac gggactctgt atgccagcga tggtcgtacc
    34681 cgcaaagatc cgtcgaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg
    34741 ctttctctga cctggaacgc gccaatggaa gccttcaccg aaaaagatca gttcttccac
    34801 ggcgttggcg ttgacggtgt gtatctgccg ttccataaag caaaccaatt cctcggtatg
    34861 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
    34921 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag
    34981 aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
    35041 cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
    35101 tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca
    35161 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg
    35221 caaaccccgc acatgaaggc gtatagcgaa gccgtaaaag gtgacgtgct ggagatgaat
    35281 attcgtattc tgcagccagg gatttaacat tcctgaaaag aggccggtgt tgctaccggc
    35341 ctcaggtcag attagccatt ctttcttccc gttttcagcc cccgatggaa atcgttaatt
    35401 cgcttaatgg ttttaaccgc acgctgcggc accgcggaag ccaccgacca gacaatcatt
    35461 tccaggcaca acaataccgt accatgtagt ggcgttttcc ccttttcatc cccgcgcgga
    35521 acatgaatca cgatactggc gtctttgcta aaacgtgaat ccagcgcgtt tgtcagcaaa
    35581 ataacaggga tccccagccg tctcgcttca cgcagcgttg tcagcccttc ccggtgcgca
    35641 gatttttgcg ccatcatgat cagaacatcg ccacgttgaa gcgcaatcag ttgctcggca
    35701 aggccaatac ccgtacggtt tagtgccgtg gcaggcaaac ctatgcggtt gaatagtcgt
    35761 gcggtatagt ccgccagaat acccgaagcg ccaatgccaa aaattgcgac ttgccgtgcc
    35821 tgtaccaaca acgctaccgc ttgcgcaagc gcgtggcggt tttcaggctc tgacaggaca
    35881 ttacaggtat acaaatgccc ttcaagaaca aagtcgattg cggtgttaac atcgctcgtc
    35941 aagttactca ccgtcgtaga catcttttcg ctggaactaa gggctggacc aagccactgt
    36001 tccagagtgc gtttcagatc acgcagcccg gcaaacccca gggcctgtat agccctgata
    36061 actgtggcat cggaagtgtt caacgtggcc gcaatttcca tcgccgtttg atcgagtacc
    36121 acttcacgat gctcattaat atagctggcg acagctctta acccaggcgt taactgactc
    36181 gcgcgagtgc gaaaacgctc accataaata tcaacccgcg ttttgtcttt ctgaatcatt
    36241 tggcctccgg taaaggtcgc cccgcaattt gtgattgtat ctgcgcagcc atcagcccca
    36301 gagatgcaaa ggagttagaa attgcctctt cacgagaaat cagcacataa cgcccggaac
    36361 gacaggcagt cagaaactga caccatcccg gcatgacttt ttccattgct gccagctcat
    36421 cctgaggttt accaccggtg tcaccgcgcc aggttgcaaa aacaaaatcg gcatccagtt
    36481 caggtaaacg ctccgcgctc acatccattc gcccaccttc aggaatgctc tcaatcagcg
    36541 gcggaaagcg aaaaccagca tcgcgtaata cccgccccag cgaatgatag ctatgcatca
    36601 cattaatttt tccctgattt gcctgaatca ccgataccgt tattttttgg ctgtccagcg
    36661 tcgccttcag cgcattgatt tgtgcctgat agcggcgttc aagtatcgcc agctgcgact
    36721 gcgttcctgt cagttctgcc agcttgcgat agatttctgg cgcgccccct ttgagatgat
    36781 caatgctcac cgtgggcgca attttttcca gtcgttcaat gggtgtatta cgggtcggtt
    36841 ctgtgatgat caggtcaggc tttgccgcca caatcgcttc aatatcaata tcagcggtgc
    36901 caataaaagc gatcgatgaa ttatcaaagt cgacacctgt caataacgcg cctgatcgaa
    36961 tgaaatggct accgtcaggc cgagttcgcc cgtggctggc gacaggaggt acaccgagct
    37021 caatcagagg aatagtgata tcaagatcgt gtaaagagac aattctcttt ggatgagcag
    37081 gaacagtgac tttgcgccct aaatcatcag taaagacctg aacaggttct gtcgcgcggg
    37141 caaagaatga caacagtatc agcagagaaa aaaataaacg catataactc ccattaaaaa
    37201 ctgttcctgc gctgccagag cagtagcaaa aagaatggtc caccaatcaa tgaaataatg
    37261 attccggcag gcagttgcaa aggcaaaaaa gccaaacgac caatgctatc cgccaggatg
    37321 actaacaacg cccccgatac ggcgctcccc agcagtaatg tcgtctgccc gccacgtaga
    37381 ataaaacgtg atatatgggg tgctatcaat ccgacaaaac cgatgttacc aacacacgaa
    37441 acgcagacgg ctgtcaggat tattggtgcg gcgacacgta acaacgccag acgagaacta
    37501 ttcaccccta acccggttgc aacctgatga ccaagcagag cgatatccgc cgtcctggcg
    37561 gtgaacagca ataaaagagc ggctggcaac atccaacagg ctgaaatacc gactaacatc
    37621 cagttagcag catgcaggct tcccgataac cacatcaatg cagtttgtac atctcgaaca
    37681 tctgcagtgg tcataaatac accgataccg gcggcgaaaa accaggaaac accaatacca
    37741 atcagaacaa atcgcgggcg ggaaatttcg cgtgcacaaa aaatcacgat taaagccacc
    37801 aacaagccac cagcaagacc agccactgga cgccagaaca ttcccagcat tggaaactgg
    37861 aaaatcagcc acagcaccgc cacgctacat ccttctttga cgccgattaa accaggatcg
    37921 gcaaggccgt tacgcgcaat ggattgcatc gcggcccccg ccatacctaa catggctccg
    37981 catagcaatg ccatgatgac tctcggcaag cgaatatcct gcacaatata acgtgcgtct
    38041 gtgcttacgt tttctggagc aaacaacgct cgtcctactt ccgatgccgg gacaaaaaaa
    38101 gaaccatgca tcagaccgaa tatcagtatt ccaaccgcaa acagaaacag cccaccaagc
    38161 aacaccagat ttttagggcg taccagtgtc gaaaacttac caacacgtag tggacgtagc
    38221 ccgacgttca ttatccgact catttgaaca ttctcgtcgc cataatgaca aaaacaggcg
    38281 caccaactaa cgccgtcatg acaccggtcg ctaactcatg gggcgtgaaa agcgtccgag
    38341 caataatatc tgccagtaat aacaaaagcg cgccgacaca agctgaaaga gggagcagaa
    38401 cgcgcaaatc cgctgatacc agacgcctga caatctgcgg tactagcaga ccgacaaaac
    38461 caatggggcc agcaatcgat acggctgcgc cacagagcaa cgcgatcgca agcagagtga
    38521 atgttcgcgt gcggagtacc gagacgccca gcccctgcgc catgcgatca cccagtgcca
    38581 acatgttaag agagggcgca aggtaaatgg ctaacacaac cccccccaga gaaaaccagg
    38641 cggacgtacc cagcgtcgcc caatcctgcc ccgctaaatc acctgctagc catgtccgca
    38701 tcgccagcag tgtttgttcg tcaagaatca ggattgcagc cgttattgag gatacgaacg
    38761 ccgatagcgc cacgccgcat aaagtaactt tcattggcgt tagccctgaa cgccctgcgg
    38821 aggaaagcag caatatcaac aggaacaata acgcgccgcc cgtagatgcc agcagtgggc
    38881 gtccaaccgg aaaggccagc ccaagcgcac tggcagcaac gacagcaaga gcggctccgg
    38941 catttaagcc taaaatatgt ggctccccca atgggttacg aatgacagcc tgcaacaaag
    39001 caccagcaac tccaagagaa gcaccggtaa gcaatgctgc cgctaaacgc ggcaaacgga
    39061 gtcttacaat tacgttgtga tcgaagtttt gcggttcaaa atgaagaaat gcctgcaaca
    39121 ccgtttgtgg tgcaataaat cgggcaccaa tccctggata taccacgcat cccacaagca
    39181 gtaaggcgag gaaaattaca gtaaccagcc ctgtgcgcat catacttttt ctacaccacg
    39241 aaacggcatg aagaacggct ttcccgtcag agtatttatt gatgcgtgaa catcaacgtc
    39301 aaagacagct tttaccagct caggcgtgca atgctccccc tcattcaaca cacgcaccac
    39361 tttcccctgg cgtaaaaata tcaacgtatc accataattg acggcaaaat tcagatcatg
    39421 caacaccacg acgactgtac gcccgtgatg acgcgttaaa tcatgtaata actcaagaat
    39481 ttcaacctga taacgtaaat caagccaggt tgtgggttca tccagtagga tataaggcgt
    39541 cttttgcgcc aacaccatcg caatccagca acgctggcgc tgcccaccag agagtttttc
    39601 aacaggtaaa tgagcaaact cctgcgtacc ggttaatttc agggcctctt ctaccgcctg
    39661 ctcgtcggcg tcgctccatt gacgtatgaa attttgccag ggaaaacgcc cacgcgacac
    39721 cagttcataa acggttaagc cctccggcaa taatggcgac tgcggcaaga tcccaagtct
    39781 acgggataac gctttcgttg gttgctcgtg aatcgcttta ccatccagca atattgagcc
    39841 gcccatgggt tgcaagacac gtgcaatggt gctgagcaac gtcgactttc cacagccgtt
    39901 tgccccaacc agaacggtca ttttttctgt gggaacggag aaagaaacac catcaacaat
    39961 gattttcttg tgatacccgg cagaaacatt gtcgaggatc agcccctgcc cttttgcgtt
    40021 atgtgccacg tgtacgccaa cttaatcaaa gaaaaac
//
LOCUS       DAZFKY010000020        39967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFKY010000020 DAZFKY010000000
VERSION     DAZFKY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(139..516)
                     /locus_tag="ACHKCL_000977"
     CDS             complement(139..516)
                     /locus_tag="ACHKCL_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFO5761998.1"
                     /translation="MNLDEEFYELQKLFAQKDLLAEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(638..970)
                     /locus_tag="ACHKCL_000978"
     CDS             complement(638..970)
                     /locus_tag="ACHKCL_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFO5761999.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            complement(1974..2441)
                     /locus_tag="ACHKCL_000979"
     CDS             complement(1974..2441)
                     /locus_tag="ACHKCL_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFO5762000.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            2634..3203
                     /locus_tag="ACHKCL_000980"
     CDS             2634..3203
                     /locus_tag="ACHKCL_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5762001.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            complement(3402..4718)
                     /locus_tag="ACHKCL_000981"
     CDS             complement(3402..4718)
                     /locus_tag="ACHKCL_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762002.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4870..5643)
                     /locus_tag="ACHKCL_000982"
     CDS             complement(4870..5643)
                     /locus_tag="ACHKCL_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762003.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5766..6224
                     /locus_tag="ACHKCL_000983"
     CDS             5766..6224
                     /locus_tag="ACHKCL_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5762004.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            6940..7722
                     /locus_tag="ACHKCL_000984"
                     /pseudo
     CDS             6940..7722
                     /locus_tag="ACHKCL_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            7842..8029
                     /locus_tag="ACHKCL_000985"
                     /pseudo
     CDS             7842..8029
                     /locus_tag="ACHKCL_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8227..9078)
                     /locus_tag="ACHKCL_000986"
     CDS             complement(8227..9078)
                     /locus_tag="ACHKCL_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO5762005.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(9152..9394)
                     /locus_tag="ACHKCL_000987"
                     /pseudo
     CDS             complement(9152..9394)
                     /locus_tag="ACHKCL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(9619..10722)
                     /gene="zorE"
                     /locus_tag="ACHKCL_000988"
     CDS             complement(9619..10722)
                     /gene="zorE"
                     /locus_tag="ACHKCL_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFO5762006.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(10726..11433)
                     /gene="zorB2"
                     /locus_tag="ACHKCL_000989"
     CDS             complement(10726..11433)
                     /gene="zorB2"
                     /locus_tag="ACHKCL_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFO5762007.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(11440..13092)
                     /gene="zorA2"
                     /locus_tag="ACHKCL_000990"
     CDS             complement(11440..13092)
                     /gene="zorA2"
                     /locus_tag="ACHKCL_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFO5762008.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
     gene            13304..16663
                     /locus_tag="ACHKCL_000991"
                     /pseudo
     CDS             13304..16663
                     /locus_tag="ACHKCL_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
     gene            16663..22977
                     /locus_tag="ACHKCL_000992"
     CDS             16663..22977
                     /locus_tag="ACHKCL_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5762009.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            23201..26059
                     /locus_tag="ACHKCL_000993"
     CDS             23201..26059
                     /locus_tag="ACHKCL_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5762010.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            26062..30996
                     /locus_tag="ACHKCL_000994"
     CDS             26062..30996
                     /locus_tag="ACHKCL_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762011.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            30996..37337
                     /locus_tag="ACHKCL_000995"
     CDS             30996..37337
                     /locus_tag="ACHKCL_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5762012.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQISSVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            37334..39448
                     /locus_tag="ACHKCL_000996"
     CDS             37334..39448
                     /locus_tag="ACHKCL_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO5762013.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT        10444 a         9020 c         9813 g        10690 t
ORIGIN      
        1 gaattgatct gacggttttc tctgacagca tacaatcaag tctaacagtc cgccttgagc
       61 gaggagcgga cactcatgat tcttacttat atatattttc ctacatcgaa tttttttagg
      121 ggaaaattat ttagttagtc acactccgcc agtcaattct gcgattagtg gttcataagg
      181 tgcgccagct tgtagtgcat tccagatttc taaaatttta tcacgatttc tccccaacca
      241 actcgaaaca tcactatcat atttcttagg taaatctccc tctatgcggt ctcctgattg
      301 tatggcataa ctggcagcat gcgcttgttt tccatagtca atatgaatat ggggaagttg
      361 atgtcctttt tcttgatata tttttatctt catgttctgt ctcttcacca agagaatttc
      421 catgaatccg ccgccactag agcgtctttt ctcagccagt agatccttct gagcaaagag
      481 tttttgtaat tcgtaaaatt cttcatctaa attcatatat cacccattaa atttcagtta
      541 gtagttatta aactcaactc tttatcgcta ggcttttcct agtccacttt tggcacaaag
      601 ctgtttaggt caaatctaag ctaatacact tgattactta cgattgcatc cgcagccctt
      661 tcacgctttc tgatggtagg ctaatcactg ttttaatgca gttctctatg ctgccaatga
      721 tgtcttcgcg tgggccgctg cgccagtctg cgcttgcgta aaaagcatcg agaaccgcag
      781 tcatgctttt agcgctgtca aaggcgcgaa tcaagtagta gcaatccagg tcatcaagcg
      841 agttgccaaa cgaaacaaca tcgatcccgt ggctttggtg taggggaaca ctgatttcct
      901 gcattatggc gtgaaaagcg gcaccgctgc ctttgcgtaa ggtgtattgc agtatttcga
      961 cggttctcaa gcgttatctc cgtgttgccc ttgggcggct cctagttgat agcaataaag
     1021 taaaccggac tgggccatga agctactgct aatcagacag tcatccgtaa gcagaagctg
     1081 cgatatgcgg ttcaaacgcg ctgtaaggct ctctggcgag tgttttaaag aaggtagtcg
     1141 aaatcatggt gcagaattcg cggcgctctc atagagtgac tggtgaagcc agttgaaaca
     1201 agcgtagcgg agttcgagca atcaatgaga aatctgggcg agctttagtt ttaccgcgtc
     1261 gcttaacttt ggaggctgat tgcctctata aggactgtct caccgccacg gaaattatcg
     1321 gatgcaatgc ttaagagggg cgcttccaaa cctaggacat ccactttttc gctcccaact
     1381 tatccggctg catttacacc gcgaacacgt tgaacgttag cagcttctgg ttcgtgtttg
     1441 gttgttacgc atgtgaggta aaaacggcag ctttactttt gacgggaacg ccataagaca
     1501 ccaaacagca gagaagtgta attgttaaaa atgttaaaac ataacttgtt ctttaagatg
     1561 taaacccttc ctcggggggg aagccggaca tatcaaccct aaggccatct gagaagatag
     1621 cctgtaggtt tgctcggttc cccttgccag atgaagatca agtcttcctc gtggtgtgac
     1681 gacctttctt gaaagatgca ttatgcgcaa aagaagctac ttaagaatcc gccgatagct
     1741 actttcaccc tctgcttggc tgcatcaaac gcttgcgcgc cctacctacc acaatcaacg
     1801 ttacgcagag ttcccatcct gacgtcttcc gctaaacagc ggtgatctcc acctacccgg
     1861 accggtttac cgcatgaggt aggtgtcagg agagaagcta gcacaccatc attttaaaga
     1921 aaagtcatat atatcatatg gctgcgttca tattctgaac gcagttatcg atgttaacct
     1981 aaaggtatga gaacactgtc gtttttcata tacaaagggt gcttcggctc acctgtagcg
     2041 tttaagacga agcacattgt gctctctggc aatagctgtc ggacggtcgt tgagcgtttg
     2101 aggtgtcttc cttcatttcc ccatgcggcg acaaccagtc cggcaccgct aacaagacgt
     2161 atcaagtggc tatcgttatc tttgccaatg ggatcagacg ctttcatcat ttcatgtcgc
     2221 tgggtgtgaa cgaatgcgaa caggttggcc atgtatacgc caccgtagcc ccagcttttg
     2281 gcaaacgata tgcagcggct caaggtgcga tcatccttct cggcatcggc gtatgaaggg
     2341 tttagcccta tgaaaagggt atacggtttt gattcgtccc agactcgtga taacgagtag
     2401 cgatacttac cgcacggtga aaaaatcgcg tctttaatca tttcggttgt ccttatgaag
     2461 tgatggtttc agcctaacat gtcttcaccc gttttaagcg ccaacaaacg accgttttcc
     2521 ggcatcctcc aaccgcacgc tttcacactg ttggaaaatt agccttggct tttccggcag
     2581 gccatcctat tttcatcacc gttttctggc aggggccgga tgaggagatg actatggcta
     2641 tttttggcta cgggcgtgtt tcgacatccc agcaggatac tgagaaccag cgcatggaac
     2701 ttgaacaagc tgggtggacg ttcgactttt ggttttctga tgtagtcagc ggaaaggttc
     2761 ctgcggtgca acgcaaagcg ttttttgaaa tgctcagcaa aatccgcgat ggggaaacgt
     2821 tggtagttgc aaagctggac cgcctcgggc gtgatgcaat tgacgttctg caaactgtca
     2881 ggacgttggc tgaccgaaac atcaaggtta ttgtccatca gcttggcacc actgatctca
     2941 cctctgctgc cggtaagctc ctgttgtcga tgctagcagc ggttgcggag atggagcgtg
     3001 acctgctgat tgaacggact aatgcggggc tgttacgggc aaaagctgaa ggcaggaaat
     3061 tgggcaggcc tgctaaaatc gcaccggaag ctcgtggagc gattttagac aaaagggccg
     3121 ctggggttag tgttagcgca ttggcgcgtg aatacggtgt ttcaagagcg acgcttgctg
     3181 cattactgaa taagggttac tgatatactt aaaacatgct ctaacacttt gcagatacga
     3241 gcaatacttg gtaaaacagc acactttccg ccagaatcgg atttttgagg tcctggcaac
     3301 agctaattgc tgagaacatt tcagtaaact gtttaactcg ctatcagccg aacactgatt
     3361 tgtatagaaa tgctaccctc gttcatacta tcaaaaataa attaattttg atgccagcca
     3421 ggttggtcat tctcaaatac ctcagcctcg gggaatattt tagtcaatgg gataacatat
     3481 ttgaccagat catgggtaaa aaagtcatcc gcagtttcat ctaccatcca cgcagagttc
     3541 tcatctatgc gtggcacgag ataatgaaca ttcaggcgga ctattttaga tttttgctca
     3601 aaatttacta tggcagcata gtttttttcc cttcgtttat cggcaacatc cttttgaata
     3661 tctacatcca aagctatatg ccctacgcct agaccataat ccaaaagctg gttatctatc
     3721 tctgctaatg tttttgttac atgtcgcgaa cgtgcatcga aagatttttc attgatacaa
     3781 cgccatgtta tcaaagaagc aagtttaact ttggttatga aacgtgagtc gcgctcatct
     3841 ggtcgtccca tagcaacaac ttggtaattt tcatcttcca gaggggagcc tttgataaga
     3901 cgagcaagtt tagtgttgac tagtaatgag ccgttttcag tgatatcttc cctgataaga
     3961 tttaagttgg ccggtctaat cgttcctttt ccataatcat cattccattc ataaaataca
     4021 cctttaaagt tttttaagtg gctaatgata tagttttcag gcgcgtcttt aacttcacat
     4081 aaatatgtga catcagtcca tacgttcatc tttttggatt tgatatgaag ttcggcaaga
     4141 tgtgagcgta tatgatgctg taatatttcc tgctttacgt aaggaccttt ctgaagcctt
     4201 ttgcattcga caaagaaata atcgccatct ccaaggcggc aacgaaactc cggggttttt
     4261 gctatccctt tttgttctgg aatgaactca acttcataac cttctgatgc atagtttcca
     4321 gcaagaacca actctaataa ggctgtgtct ggtaatactg ttgtattctt aagcattctg
     4381 attgcgcgat ctctagcgcc agtaattcta tcgagcgatt tagcacacac tcctaattgt
     4441 ttaacccatg gtattatatt tgaagcatta gtaacttcgt aagaccttct attatcgata
     4501 agcgattttg cttgtgcgaa ataaccagca accacgtcgg aaccatacca ttctgggtca
     4561 aactgtttcc cgaaattggc agctttttgg gttgtagcta tgtaaaattg ctgtgctttt
     4621 gctaagcgct ggtaaaagtg ttgtttgttc tcttgagttg atgctaacca ctcaagacca
     4681 gcaataacgt cagaatctag cataacaccg tcattcatct ttattcctta gttcagccct
     4741 gaaaggcact agatcaactg agacagatta tagttacgga aagataacgc gatgaatata
     4801 cgatggtaag cctccgcttt ccactctatg ctggcagctg agttctatca tgttcaggca
     4861 cctacgtttc tagaagttgt tcatacagtt agtataaata tacgtccctg aaatctttgc
     4921 gtcttgcttt ggatgttcat atacgtagtc aacaaaagtc ttatagagcg ttatcatctg
     4981 ctgggtgacg tcggccttac cgttcatgtt atcaagtcgc tgataatatt ctcttttcgt
     5041 catcccactg ttacggcctt cggttacgtt tgcggtaaat tgagccatac ccatgcacat
     5101 ttgatcttta ttgttattca tttgatcttt attgttattc gttttatccg ggtggagagc
     5161 gccttccaac actttatcac cgctgatggc atgttgcgcc cacgctgaat tgttagcgat
     5221 aaactttttg gagccgatgg caataccgta ttcaacgcga attccattgt cgtgataact
     5281 gccatctgcg tcttttgtcg ctagctttaa agatatacgg caaccgggaa gcgttttgtg
     5341 ttcttgcacc gagataataa cggtgtccgt atcgcaatca gcaaagctgt attcggggtt
     5401 aacgtaagtg ttagttttga ttaacagccc ggtaagaccg tttttatagc cttttgccgg
     5461 cagcgttttg aaggcgctgc gttcaccctt tttgatcgct tcccggaagt gatccatacc
     5521 gatgcgcgtc gccagttgat cgatgctttc tgctgcataa gatgcagatg acgcagcgat
     5581 taacataatg gtcagcacag acttggctga aaaaccgggc ttactaagcc gtgtttttga
     5641 catgttgtta tccctcagaa tccaccccag cggtgtcagt tatcgcatca tagaccattg
     5701 attcaaaatg tcactattgg agcgtggctc tttagtggac atctgcgcaa attcaggtca
     5761 tgaaaatgac cctgagaaaa ctgtttggac aacgagtaaa agagttgcgc attgccaccg
     5821 gaatgagcca agaagccttt gccgatcggt gtggttttgc tcgtagctat atgagccgca
     5881 ttgaacgcgg tggctctaat gcgtctttgg atgcgattga ggtgctggct aatgctctga
     5941 gtgttgagcc gtggcgacta ttggtgtctg attcatctga agatagtgac ccggaattac
     6001 tggttcctta tgcagctgat ggctcgtgtt ttcatcccgg gctggcaagc actcgcgatg
     6061 gctcgtttgg cgtaggtgat aaaacggctc agaagcggtt tggcacgttt tctgaagctc
     6121 ttgagtatct tcgtagcatg gagacagcaa aatggcgtag gcctaatcct agcggcaact
     6181 ggggtattgt atctgcggtt cggtgggaca agctgagaaa gtagctcaag tgtaagttta
     6241 tgaattttca tgctgtatgg atgaacagtg ttcattagat gctgatagcc tcacgtttca
     6301 tcagctcttt gtttctataa gagtgaataa aacagcaaaa tgcgggtgcg ttacgtaggc
     6361 gttacggtgg cgttttactt tgacgctgaa tggcttaatt catataaata acattgagtt
     6421 aaatagttat tctgaacaag gtctgacggg gtttaactgg tttcaaaatc cgaacgcagt
     6481 ggcgtttact taacatccac atagctgaca aatgttcttt cctcccgacc cactagacct
     6541 ccataaacat caaaaggcag gtatggcacg ttaatgattt gtgccgccac gaacactcgg
     6601 tttgattccg tccagcggat caaacttaag atgtagcccc tagaacaaaa aggtcctacg
     6661 attgttcttt taaaggctaa taggaacgtg attacagcag gttatggaga cttaggttcg
     6721 agtccggcct tcggcaccaa aagtatgtaa atagacctca actgaggtct ttttttatgc
     6781 ctgaaatccg cgccacgcaa ggcttttcca gcttttctcc tcaactgaag tcaacctgaa
     6841 ccaacccaca tcaacaggct tgtgagtata ctaatgagta tatttgccga ttcgatattg
     6901 cttgtatact cacgagatac caatggaagg aagaccatta tggccctaac ggatactaaa
     6961 gttcgctctg cgaaacctga ggagaaagag tattcacttg ttgacggcga cggcatgtcc
     7021 ttacttgtaa aacctggcgg ttcaaagtat tggcgatttc gtttccgttt tggcggtaaa
     7081 caacatttga tggcgttcgg cgtttacccg gatgtttcac tggcggatgc gcggaagaaa
     7141 agggaagagg ccagaaagct ggtagctgct ggtatcgatc ctcgtgagca taagcgtgct
     7201 gtgaaagaag agcaggcgaa agagattatt actttcgaga aggttgccag agagtggctc
     7261 gtaaccaacc aaaaatggtc ggaagatcat gctaatcgtg taaaaaagag cctggaggac
     7321 aatatcttcc cgacaattgg tactcgcaac attgctgaac taggcacccg tgatctgtta
     7381 attcctatta aagccgtgga gaaatccggg cgtcttgaag tggcttcccg ccttcaacag
     7441 cgcaccacgg ccatcatgcg ttatgcagtg caaagcgggt taattgatta cgatccggcg
     7501 caagagatgt cgggggcggt tgcttccagt aatcgacagc atcgtccggc gctggagtta
     7561 aagcgtatcc ctgagttgct tgacaaaata gacagctaca ccggcagacc gctaacccac
     7621 tgtacgacag aactcactct gcttatcttt attcggtcca gtaagctgca ttttgctcgc
     7681 tggtcagaaa tcgattttga aacgtcaatg tggaccatcc ctcttgattg gtattgcagt
     7741 aagcagaggg ttggactggt acagtaaata tcccgcataa tcgtgccatt cacatttaga
     7801 gatcctccgg cataatcaat ctgccaatga aggagatcgc tattcgtaaa gcccctttta
     7861 ctgagcatca gattatcgcc gtgattaaat cgcttgaata cgggcgaact gttaaagatg
     7921 tggcttatct gaagccactt actacaactg caagtccaga tacggcggta tggaattttc
     7981 tgatattaaa ataatcaagg atcttgagga tgagaaccaa cgtttcaaat agatgtttgc
     8041 tgatctcatt atttggtcgg gatttttaaa gatgtatgga actaaaacgg gtctattttc
     8101 agagggattt aactgaagca gaaattaata aatcataatt tataggtggc tggtatttaa
     8161 tattcctgca tcatatctct gatgcaggag cagagaatgg ctataaatta ttagtaaaca
     8221 gaatgtctat ctctcaccca caaacccctc ataagggtaa tttttacgca cctgcggcaa
     8281 cgcaatagtt ttcccgtcaa aatcccagaa taatctctca acgatccctc cgccgtttac
     8341 cgcatccgag accagtaccc gcatattttc atccaggcca atcgatcctt tatcaaaggc
     8401 tttatggtgt attgcgcaaa gcgccagacc attggggatt tcacaagggc cgccgtgctg
     8461 tttccatttt atatgcgccg cttccagcgc gacggtggtg tcatcgtggc gcatattaaa
     8521 gccacatatt gcgcactggt agttataagc gcgtaataca tttttgcgaa ataacgggtc
     8581 gcgctgttta cgggtttgct ggagatcaaa acccagctcg tcagcgattt cttcctgaat
     8641 actctcggtg aagtgcgctt cgagtatctg ttgcgccaga gtattgatga gttttttatt
     8701 gccggttacc agggcgtagt gctgttcatc gaaaccgcct gcaacgtggt attcgttcag
     8761 ttccttcact ggcggttgtc ggctgctccc tgctgttgag caacgctcgg cattgtgtaa
     8821 ttgccagatt ccgtcgcctt gtaatcgcca gaacggcata tcaggtcggt actgtgaacg
     8881 ctgtggccca aaacgttcca gtaaactgtg cagaggttcg tagatttccg tgccataatc
     8941 gaaaaggcgc ggatgtccat tcaggtatcc cgctaatacg tatagcagta acaatggttt
     9001 atgcggcgcg cgctgttcac ccttgtgcca gatttttatg ttggcaattg cctgctgtag
     9061 cgatttactg gaagccattg atatcccagg cagtgaaata attgcaatca tgctcgcaaa
     9121 ttttctgctg ttcaagcctg caagtgaaga tccattgctg tgttgaggga tcaaaagaca
     9181 acaaaaactt attgaagaaa taatcttctg cttttttgtc gaaataatgg gcattgatct
     9241 tgtctatagc tgctgaatct tttccagcct cgttgagatc ttcaaaatag ccattgaaga
     9301 tgttctggtt aacaccgcca gtaacataag ccttgccgtt gtgtacaaaa gtcacattac
     9361 ccgccatgcc catcggcgcg tgcgacatca atttattgct ttattcattg tgactgtctt
     9421 ctgtctacca agctttaacc ttcacccata tgagggaagt tttaagtatg aggcgaacca
     9481 tagcatatgc ttttatacct gttacaccat aaaaatcaga caagttatgc cataaactac
     9541 ttttgtaatt tgagccacaa atcagggaaa ttttatttat cttccgtgaa agtcttaact
     9601 ttcacggagt tttagaattt acaattttgc tggcgtaaag gctcctgttt gtatttcttt
     9661 aaccagctca cccacggtat atattctcca tccttcgact tcggcgggag taaggttaac
     9721 tgcaatttta aggcctggcc atgcagcttc tacggttgca ataatttcat gttctgagtt
     9781 tggaagatct aacccaggaa caggaggttg aaagccttta tgttgcatca tattaatatc
     9841 accatgcact gatggatcag tcaggtcata tatttcattc caggattctg cttttaataa
     9901 cccttgttgg gaacgtaaac gttgaatgat agccatctct gcttgggtaa cgaatatacc
     9961 ttcgtggagg ggctgttttc gatggcaatc cttgcacaga acaagaaggt tttcatggtg
    10021 attatcatat ttgatgccat ttttatgatg gacatggcac aagtttttgg cggttgataa
    10081 attcacgcca caatcattac aaacataatt tgccttttct cgtatttctt tagatatttc
    10141 cttccagttt tcgacatacc cactatttgt tttgtcatag atacccttag gcatatgacg
    10201 aaaacaagta ctataatctg aaaaaaactc atttaatgag aatgatttaa aaatctctct
    10261 ttttcgttgt ttatcaatac ttcctttata attaagtcgt tcgaggcaat tcatacatac
    10321 ccgtaaatca acatcctggc tccgacctga accatcatca atttcgaata aaccatctat
    10381 gcggttagtt gcatgatacc tttcaaatcg atttttatgg cgcatttcat caagagttct
    10441 gcaccaggca atatgaaaac gttttccttt ttcgccatct acgatagccg catcataacg
    10501 ccctgaatgg tcccgaatat ataaaagaac ctggcggcca tgataagaga taagcccgct
    10561 accagggtca agatcttcga tactaacttc tttacccgtg gataactcaa tatctatagg
    10621 atcaaagtca ataggggatt catccagaaa aaaatcgact ccttctggta acattttctt
    10681 ccctaattga ataagttctg aaatgtcgat agataatttc atctcttact cctgaataat
    10741 ctttctaatc tgcaactcgg catttgttac aactttgaag gtgacacgac gagagcgatt
    10801 aggatcctcc ttgccggttt tatcaagaat gggatgtgca gatgaataac ctactgcggc
    10861 aaatttactt ttaacccatt gttggtgtgt cgcgatattt tttatgtcat aaacgtattg
    10921 taatactgca cgtgtacgac cttgcgatag tgccatatta ttaaaataag caatatcagg
    10981 attcgttgtt cctgtccagt cagtacttgt gtgaccttca atgcggactt cagtaatatg
    11041 ttcctgataa ttatctagaa cttttaggta gcgaggaaag aagtcgtcaa gaatgagttt
    11101 aaactttggt tttagctctg tgcttcctaa gccaaataaa acatccggtg atttaaatcg
    11161 aacctccaga gtctgtttat cgatctctgc atcccagtct tgtaaatcct ttgcaaactc
    11221 aatatccaga gcattataaa gctgtaactg agcattctcg taggctacgg caacttcttt
    11281 aattttttct ttttcaatac gtacgtagtg catataagca atagatatga acataaaaac
    11341 catcatcagc ccagccatta ggtccgacat ggatacccaa tgttcattat cttgatcttt
    11401 tttaggtaat tgtttcccta taatcttatc catgatcgat tacccgcgat gtttattagc
    11461 agaaattaat gcttgtaact gccccataac tatttggtaa tctttaataa gtttttcata
    11521 gttaccgaca aaacctttgc tgattgaaag cagagcatta cctaatgatt gcatttcacg
    11581 gttcatttca cgttcagttg cttgctcaaa agcatgtaac tgctcgttga tggatagagc
    11641 aaatttttca attgattctc tgacttgaga agtcagccct gcacttgcct tatcaaatga
    11701 tgtctggatt gaatcaaaca tattatcggc attactggat agttgatggt taatcttttc
    11761 accagtctta ctgaacatat caatcacatt cgtcgtattt tgttccatat ttctatgcaa
    11821 ttctttcgag tgcgactcta gtgatttcag gaattcttca ccactttgtt tcatttcagt
    11881 aattgtttca cctaacgtgc tggttagggt ttcggaggaa ttgctgacat catgtgccat
    11941 cgaggcaaat gcttgttgtg tttgggtaac cgattgtctg aatccttcgg taccttcatc
    12001 cagggcaacg cgcattgaat ctgcattaag aagtatttgc tggctggttt gctcaagaga
    12061 tgcactaaca tttgcagctc cggatttcag cagttcaccc acttcagcca ttttgttctg
    12121 tatttcaggt aatacggttg tagctttatc gcggatggcg acaaaggttt ctaaatggcg
    12181 ggagagttcg ctgatttgat gttggttcac ctgaagcact tcacgcagtt cagacatagc
    12241 cagaggaatt tctttacatt cttcccaaat ccctgcaacc gcagtttttg tttcaaccag
    12301 ggactcgaca ctttgttgat attgttctga catctgctca atttgcgttt tataatttcc
    12361 ctgccactca acaagttttt ttacagaggc atcaagagct ttaaagtttt caccaaactg
    12421 ttcagttaaa ttttgattaa aatcaataat gacttgtcgc aaagcatcaa taatttgttc
    12481 tgtagcacct tttgccatta gatctgcaaa ttgttcaagt tgttcccaaa gcttattact
    12541 gaaatgagtg tgattagcta attgtgcctg cgaggaatcg ctaatctcag tacgtagtaa
    12601 ttttatttga gcaataagag aaccttcact atcaccgtta atacccgaga ctaatttagt
    12661 cagagtctgg ttctgctctt ttaattcatg atagatatgt tcaggtgtaa cagattcagg
    12721 gttattttct gctaacgcac ttcgtttatt ggcaaaaaag aaagcatcca ttccattaaa
    12781 taaaatggca aaaaacatac ctacaatgct tgtaatgaat gctgttttta ggccacctaa
    12841 taatacgggg atgctggtat caatactttc ggtattaaaa ttgagtaaac caataataat
    12901 accaatgaaa gtacccagaa tacctaatgc ccccattaag gttggagcat attctctaaa
    12961 cttactatgt atattttttt tgtcacacca ccacgctgat aaaaatacga atataacagc
    13021 ccagatgaaa atattggtga tcagtgtaga gtctatcgat tgaaacaact gctcaaaaag
    13081 ctgcgctaac attttgatac ctgtgtaaat aatggatagt tgtaatcgtt taaaaaataa
    13141 acaaaacaca cataatttat atggttaagt tcggtgtagc gctcatgatt gtaacaagtt
    13201 ttttcgagat ataaatgatt tgtctcattt ttagcgagtt catcgggttg agctttttta
    13261 tgatgatagt aacctgttac gaattgtatc aaaggaaggc aggttgcttg tgaaaactcc
    13321 agttaaccca cttttgcaat ggcttaacat gttttttagt cgccgttcat tatccggagc
    13381 tgatggacgg gcgttatatg cttaccgttg tactgataca gagtacgaaa gtttggcaga
    13441 actactgcgt acatatgccc cccgtagtta tccaagaacg atattcattt cctatagcga
    13501 tgttctattt agcatatatg ccgcagagtt tatccgccgg actcatacgg tcggacaccc
    13561 taaatgggac acaattttag attctattaa ctggaaagtt ccgtatgttc atcgacagaa
    13621 gctggttaat gatggcattc gctactggaa aagaaagata agaaacctgg gacaagcttc
    13681 gggttatctg catacactgg cttgtgaagg tggtttgcct atccgcatga ttgaaaatga
    13741 gagcggttat ttgattacct atttcagaag gatatatcag gcgcttcgtg ggcaatcatc
    13801 tcaatatcct gccgctaaaa ttgcacaaga attaggcgat acgattcctg tcacaatgca
    13861 aaacgaactg gtctatgaaa tagccggtga attctgcgag actctctgcc gattacttag
    13921 tgagcatcct cctcatagca gtgatcccgt ttctgcatta cgtaagctct ctccagactg
    13981 gcatcttcaa cttccactcg ttctccccga agcgaatgct gcagagattg taaggcgatt
    14041 actttctcaa tcttctgaaa tacgcagtgc aagtagtctt caggttgaaa gaatctgggt
    14101 cgatgttgat gacagttggt attgcgatgc ccggttccga ttcccggcca caatgcgtac
    14161 agaacagtta acctctttgt ttgaatgcca tattcagccg gagcagaccc gactcatcat
    14221 ttcaggaaaa tggaaaaatg gcggggcaag gttggcaatg ctaagccgtt atgagcagca
    14281 agattggcgg gtcgagttat tacctattgc gatgcaaaaa ctctctggtg cagatgcaat
    14341 ggccgaaatc tcattgtcgc ttcatgaagg tccaattctg ttaggtcaca caattccaaa
    14401 aggcggttat gaacttactg aagaacttcc ctgggttttt gaagcgatga atgagagtga
    14461 atcgcagcta aaacttgttg gtatggggtc tgtgagttcc aggctaaatg ctctgtttat
    14521 ttcgctaccg aaaaatagcc atttagatat tagtggagaa ggtgagtttg atatcccaag
    14581 gttgctgaaa aacagtgaac gcagcttaac taaaataagt ggagtattta gcgttgtttt
    14641 acatgatggt gctgtttgta caattcgcac ccagcaactt tatgattctg cgattgaata
    14701 ttatattaaa tcgacagaag ttgaactggt taaatcggat tatcctgtcc atcgagcctg
    14761 gccgaagatc ggttggaaaa aggatctgca atatggcatt gtaccggaga aagaactttt
    14821 ttggcgttcc attcgttcag gtaataatgc ctggtattct gtcgcatcag aaatgccaaa
    14881 aggacagata gaagtccggc gaatagttaa tgatgaggtt ttatttagcg gtaaagttgt
    14941 agttttacca gcagacttcg atattaatat tatacctgag agtgctcagt aaggcattat
    15001 tatgctttct ggtataacgg atactagaat tgataaatat tcaaacaatg aaaaagtaac
    15061 acttaagtcc gattattcac aaaacgagtg tgctatttat tataattcat cgctgatgct
    15121 ggaaaatacc gttgatttac gggtctcctg gaaagatggt tctaatctta aactattatt
    15181 acctaagccg gttagcggtg gccgatttgt aactaatgat ggttctgttc attttgatgg
    15241 tgtggcatct atagcacatt tgcatggaat agatgctgag ttattaacca tatcatgtgc
    15301 tggaagagga tatcttaata tcgagttatt ggatgaaaat ccagtagctg aaaaattccg
    15361 ctatttacat gccgaccttc ctcttttatc aggacgcaat gacaaattac aacaaatttc
    15421 actttatgag aactataatt tgctaaatgc tatgctggca tgtgcatgga acagcaatag
    15481 tacattatgt gttgattttt actctgacag atttggaaag gataaagcaa cactcaatat
    15541 taaacgctac gatggtagtt ttattgaaca cgatcaaggg ttactggttg atataaaaaa
    15601 ttctgttgtt tttcctgcaa atagaataga cgaactggtt gttgatgcta tttctcttaa
    15661 gaatcctggc ttacacatat cattgttaaa aaaagatgag tttgcttatg atctttcagc
    15721 tctgaatgtt caggatagtc catggttaat tgtgggaaaa cttgatggta cagctcgcat
    15781 tgcaccagta attaaatgga tgctacctgt attgcagaca aatgatttat tactaaatgc
    15841 tctatgcgaa gcagacccag aacagcgcaa aaaaaatttt aatgagctaa tttttgaaat
    15901 agataacaac ccattgcaaa attattgctg tttattaaca gagtatatta agaaatacaa
    15961 aatgaataat ggcttatcct tgctggatct ggacttgttc agaggtattt cgagtaatta
    16021 ccgcgtggtt gttcaattgt taatatcatc atgtctttct ggtgatagcg atacgattta
    16081 tgatatacag gaagaattac ccttttcatg gggatggatt cccgtttcaa tctggaaaga
    16141 tgttttccaa aaatgttgga cttatctgga gaaacagatt aacgataaaa cattagcatt
    16201 acatatattg caacccttta ttgcttttat gaaccatcgt gcacatatcg atcgtcggct
    16261 ggctccgatt gcgaatatgt tacttacata tagtgagagc ctaccaaccg gttgtgatgt
    16321 attgccaact gttagtcgtg agcagtttaa tgaagctaaa cagatgctat taaggaaccc
    16381 cgacagcttt gggcgtatca gtatcttccc taaagaactt tggtctagtg ctattactcc
    16441 agagttaaaa tctgttttta ataagctttg gattgaagat aaatatcact cacggcttga
    16501 aaaacgtttt aatttgatgt tagtcgcagc gctgttaacc caaaaagata ataacctgat
    16561 acatcaactg tctgcgcttt ttgaatttca ctatcagcaa gccccacagc aattaggggt
    16621 aatctatcaa tattattttg aacaagcagg agtatgtcat tgatggacgt tcagcaactg
    16681 cattatgcgc cacttctaaa tcagcttcat cagcgtgcgg tgaggtcggt ggtaagtcag
    16741 ttggcattgc ggagcaagtc gttgactcat tatctggaat cacaatattt tcagcctccc
    16801 ggtcaggagg ggtcactgct tgcagacccg gtattcgaaa gtacattcgg ctggatgcct
    16861 gcggatgaaa caatggagga tttgcgggac aagctgatct ccagcccgct ggttgatgca
    16921 ttgagcaaag cgccagtgcc atttcgtcat caacttactg cgtggcgaac gattctggaa
    16981 gacaaaaaat ccttgttagt gagcagcggt acgggatccg ggaaaacaga atgctttatg
    17041 gtaccggttc tggaagatct tattcgtcag caaaaatcga cccgccagtc actaacaggg
    17101 acacaagcta ttttcttgta tccgttaaat gcattaatta acagccaaca ggagcggctt
    17161 cgcgaatgga cgcgcggatt taaggggaaa atacgcttcg cgctatataa tggcgaaacc
    17221 cggcatacca aatacgaaat tcaggaagat cagttgaaag tacctgaaca agctttgagt
    17281 cgggaagcca tttatgaagc accaccttcc atcatggtga cgaataccac catgctggaa
    17341 tatatgctga ttcgtcgtaa agatgcccca atcatcgaaa agtctcaggg gatgttaaag
    17401 tatatcgtcc tggatgaagc ccatagttat attggttctc aggctgctga gttagcgctg
    17461 cttttacgtc gtgttatgca ggctttcaat gttggacccg gcacagataa acctgtgcag
    17521 attatcgcga cctctgccac tattggtgaa gactctcctg aaggtaataa ggagcttgct
    17581 aaatttgtcg ctgatctcgc gggcgttaca gaacgtgatg tcaaagtggt tcgcggttat
    17641 cgacaaatac cgcgggtatc ggagtcgctg attcgtcatg aataccccac atctttaact
    17701 tctcttaaat ccctgagccc tcaggatctt taccaacagc tttgtcatta ccgtgtggcc
    17761 cagcaattgc gtcaggcgtt aactcatccc ggacggcagg cagtacgttt gagtgagttg
    17821 ctcaatgttg ccagaagaac ctggccagat attaatcacc gtgagctact gcaactcctc
    17881 gatctaatgg caagatcgcg agaaggtgaa ctggctttta cgccattacg tatgcatggg
    17941 tttatcagaa cactggcggg tctatgggca tgttcaaaca aacaatgctc acataaagca
    18001 catgaattaa atcagagtga ctggccattt ggtcaggtat ggtttgagca gcgccaatat
    18061 tgtgactgtg gagcgccggt atttgaagtt ttacgttgct ccgggtgtgg ttcagcttat
    18121 ttgtctgcca aagaggagat gcgcggtgat ggaacgaact ggttggtagc gcaaccagcc
    18181 gctgccgaag tggatgagtt tgctcttgat gtagatgttt attctgaaga tgaagacaac
    18241 gacgaattag acaataactc tcaatttgat cgtctgattg cgagcgatgg tgaaaaatat
    18301 atcatttcac tggaagaaac gacggcgggg aaaattgata gtgaagcgca aaagcactat
    18361 gaaataaacc tgttaagacc ggaatctcga ggtgagggac gcaataactc ctttgcttgt
    18421 gtttgctgtg gtgacactca acggaaaaat aatcctttat tccgtccgct tcggctcggc
    18481 gcgccctttt ttttaaatga aattattcct actctgctgg agttttctcc attaccgcaa
    18541 acaagagaag agcagttagg accgtttaat ggtcgacgct tgctcacctt tacggattcg
    18601 cgtcagggta ccgctcgcat ttcagcccgc ttacagcagg atgccgataa agcgacgttg
    18661 cgagctcttt gttaccagga gctggcaaat attacagagc cttcagcaaa actcagcccg
    18721 gcacactgtg cactgttaga gaaagttatt agccagtttt ctgtcctccc tttaaaaaat
    18781 tccatcgacg cgctgaaaaa agcgatggct gcgaatgagg agcttagccc gacggacatt
    18841 caggcactta aaattattga gccgatggtg agttccttaa gcacggagca tgctgaagcg
    18901 atgcaaatac ttcaggctaa ctctggcaat cagcagaaga aaaccatgtc atggcaaacg
    18961 ctgtctgaac agctggttgg cagtgtggac cttgccgata gaatgagagg cagttttaaa
    19021 gaattgtctg ggcttgatct tactaaacaa cagtttgcag atttttgttt atacagcgag
    19081 tttggtcgcc gccctaaaaa tgcctggacc ctcgaaagtc tgggtctggt agccattcac
    19141 tatccattca ttgataaagt tacaacctgc ccgcaggact ggaagacatt attacccgat
    19201 cccgataagc aattagcgga atggcgtaat ttattgaaga ttactcttga tttctttatc
    19261 cgtgaaaaca gtgccgtttt ttacgaaaac gagcaatacc ctcgctggat gggggcacgt
    19321 ttcccggtaa aaatgctcca ggggccagat aaaaaaaaca aagggcagaa acgtgatcaa
    19381 ctttggccac aaatacgcga tcgccattat aaccgggtca ttaagctatt aatcgcagtc
    19441 tttccggcaa tacaacctga gcaggctcac tggaaatcgt tagttaacca cttaatgatt
    19501 gaggtctggg atgcaattcg accctgctta cgtcagtttg agtcgggtta tcagctggat
    19561 attaaacaac aagctgaatt ttacagtcca aagcaagtgt ggcgttgccc ctatactcga
    19621 cgtgcgttag acgtcacgtt gctgggatac tctccgtatt tgccgggtag caaagaaatt
    19681 gcacctgaaa aagcagtgtt gattgaaatg cctgagcttc ctgtacgtca ctggcgtttg
    19741 tctggtggtg gagaaatcgc ccgagaagaa cgtctggagt ggctggaaag taatgcgctg
    19801 attcaacatg cccgcgagga aggattatgg tctactcgca gcgaccgact ggccctgaaa
    19861 gatagctggt atcgtctgga ggaacactct gcgcagagaa cgcctgagca gaaccagttt
    19921 aatgaaaaac aatttaagtc aggcaaagtt aatgtgctga actgttccac gaccatggag
    19981 atgggggtgg atattggtgg tatgagtttg gtggcaatga acaacgttcc tccagcgccg
    20041 gcaaattatt tgcaacgtgc aggtcgcgcg gggcgtcgtg gagagagtgc atctgctgcc
    20101 attacgttat gtaaaaacac ggcgcatgga atggaggtat tcaaagaccc cctctgggcg
    20161 ttcaacacta ctgccagtgc gccacgggtt cgttttggga gcagtagtat tgttcaacga
    20221 catgttaatg ccctggtttt aggtctgttt ttacgagctg aagttcctga tgcgacaaaa
    20281 ctgagttgca aatggttctt tgaaggtgat gagagccagt gtcttcgttt tctccattgg
    20341 ttaaatcacc aggccgatca gctggcggat aaacttaaac gccttactca gggaaccgta
    20401 ctgatgtcat tgacggcaac ccagctcttg acccgcacac aagcaatgat gcagcaggtt
    20461 gatatccgct ggagaagcca acttgcgata ctgttggaaa atatcgaagc gttgaaggcg
    20521 gataacagcg cgtgggaaga aacaccagca ggtaaagcca tcgcttatca gcttcgtgat
    20581 tatcgcggtg catatctgtt ttctaaactc attagtgaag cgttcctgcc ggggcatggt
    20641 ttcccggttg gcgtagtcaa tttcaattat ctgacggcag atgagctgga aaaacgtcgc
    20701 gcaataaaag ccactcaggc tgaccctaat gaagggggag aaagcttttc tcgtcgtatt
    20761 gaaaaattgc ccagccgtga tttgcctaca gcattacgcg agtatgcgcc tggtgcagat
    20821 gtggtattgg gcggaaaagt atatcgttcc tcgggcatta tgcttggcaa ggtgcttgcc
    20881 agcgggcagg agctgtcagg cgatcatcat attccgtggt tctggcattg ccgtaaatgt
    20941 ggtgcggggg caaccagtac tacccatcct gtcgaatgta gtcactgcaa agcggatata
    21001 cagcaacttg atgtgaagcg ttatttacag cctgttggtt ttgcgacaga tattcgttat
    21061 caggcacata atgatgtatc catgcctgca caacttccct ggaaagatcc acgtgttctt
    21121 gtaccgtctt cggtgtgggt ttctttaccc gatgctgggc taggacgtta ccgttttagc
    21181 catagtgggg aattgttcca cttcagtgaa ggggaatttg ggcacggcta cgcaatttgc
    21241 ctctcatgtg gtcgggcaga atctcaaact caaccccagc gtacccctga aaatctaaag
    21301 aacccagaga gggaaaatac gcattacctg ttgcgagggg gcagtaacga tcgtcagggg
    21361 agcaataaat tgtgccacgg tcatgtgcat aaagatctgt ggttgggata tagcagtcgt
    21421 acagatatgg ttgagctgca actcaatgat gataatggcc tgttgatccg tgatgaggtt
    21481 gccgcgcgtt cccttgcggt cgctttgcgt gaagggttag cgcataaatt gggtatcgaa
    21541 aatactgagc tgggcgtgac gacgcagcaa gcgcgcgata tcaatggcta taccggatat
    21601 tccatcttca tttacgacaa taatgcgggt ggtgcaggtt atgcagtgca attgatcgac
    21661 cattgggctg atgtctttaa ttacgccaga aaattgctcg attgcagttg cgataaattc
    21721 tgtcaccact gcttgctcag ctacgacagt cagcattatg tcaacagatt agatcgtcac
    21781 catgcattaa ccctgcttac caatgtacgg ttgcagcgac ttaacctcgc gccagaatat
    21841 cagtacttcg gcgatggtag ccgggttgaa actaaccctc tgagtttacg tattgcgcaa
    21901 tgcctgaaca gcgagattta tgactcctgc tcgctggttc ttgcggggcc gcaggaacag
    21961 tgggactttg cccaatggcc attatttaaa gagttgctcc agtttgcctc ctccggtggg
    22021 aatgttgagt tgctggtggc cacgccgctg gcaaacttaa ctgatagctc cagacatcag
    22081 ctttccgcgt tggcggcgat gccgggaggt cgcttacaag tgaaatccat tgccactgcg
    22141 cagctcatgc aaggaaaggg gcgctggctt gcgcaggtga ctcgcgaagg gcaaagccaa
    22201 cagtgggccg cagacgacag cgctaccgtt gcacctggtg aattatgggg acaaagtgcg
    22261 tcttcaccag tagtgacact taaaggaacg tccggaaaaa ccttctcggg tcagacctta
    22321 agtgcagaag atttactgcc cgccttaccc accggggcgg tgcgcatcaa tctctgtgaa
    22381 caactggacg ggccgctgga aggttttggt tcgcgtttct ggtcgttagt cacgcaacag
    22441 cacgctggct ggaagcaggc atttacccgc cacaaagaga taacgcatgt tgagtacagc
    22501 gatcgttatc ttaatagccc attcacggcg cgtttgttag gtgaaatctt aaccgagctg
    22561 gttgaacagg gaatggcgga acgcgccagc ctcactgttt gcgtcaaaaa attggattac
    22621 aactctcgcc aacacgacgc tctctataac gcgtggctta atgaggaaga tcgtcagcag
    22681 gttgtgacca ctttacttga agaaggatat ctggggcctg cgtggccggg agcaattagc
    22741 tggttaacgg gggataacca gagtacggag catggacgag aactgacagt caccttcagc
    22801 gatggcagtc agcattatgt cctgctcgac atggggctta gctactggcg ctgtattgaa
    22861 gataccttct ttgattttgc cctgcgagta ccgcagcaag ttgaacggct ggcaaacacg
    22921 cgagcgcgag ctgttgcccc gggtaatgac cttaggagtt atattatcgc tggataaata
    22981 attgctcccc tggggatatc ttccagggga gttgacccag ctcacttttt aataattttt
    23041 gtttgaaaat catgcgcaat ttatgcgcat tccctcccat tttgtcactt tgccgggggc
    23101 cttcatgata tctttaaggc cctctttttt atcgcagtgt gccgcgccac atttttcagg
    23161 atgacgttac cgccactgcc ccaaatagac agaaaaaccg atgaataagc aaaattatgc
    23221 acctggtatg cgggtggtta ttcgtgatgc cgaatggcgt attcgccggg cggatgacag
    23281 tggtgatggt gggtatttgc tgacctgcga tggtatttca gagcttgtgc gcggtaaaga
    23341 agggttattt ctgaccaaac tggaacaaaa agtagagatc ctggatcccg caaaaacgca
    23401 tttggtggaa gatgaatccg ccaattatca agcggcacag ttgtatattg aaagtcagtt
    23461 acgccaacgc gtaccgacag acagtaaagt ccattttggt catctggcgg cgatggattc
    23521 catgccgttt cagctcgatc ctacgcggat ggcgctggca caaccgcgtc agcggatact
    23581 cattgccgac gctgttggtc tgggtaaaac gctggaagcc ggtattctgg tttcggaact
    23641 gatccgtcgt gggcgcggaa aacgtattct ggttctggcg gttaaatcga tgctgacaca
    23701 attccagaag gagttctgga gccgttttgc gatcccattg acccgcctgg attctgctgg
    23761 tttacaaaag gtgcgtaacc gcatcccaac caaccacaac ccgttccact attttgacaa
    23821 gaccattatc tccatcgata cgctgaaaca agacattgaa tatcgtcacc atctggaaaa
    23881 tgcctggtgg gatattattg tcattgatga ggcccataac gtcgcagaac gtggaaccag
    23941 ttcgttacgc agtaaactgg caaaactgct cgccgggcgc agcgatacgc tgattatgct
    24001 ttcagcaaca ccgcatgatg gtaaggctga aagttttgcc agcctgatga acatgcttga
    24061 cccgacggcg atcgccaacc cgaaagagta tgaatacgcc gattttgccg ataaaaatct
    24121 ggttgtccgt cgcttcaaaa aagacgtgaa agatcagatg tccggcgagt tccccgagcg
    24181 caatattgtc aaattgactc gcctggcatc cggtgccgaa gaagaagcat accgtcgtct
    24241 ggttgagagc cagttccgtg atgatgacga tgaacaggcc cagtctaaca aaggtcgtct
    24301 gttcaagata accctcgaaa aagcgctatt ttccagcccg atggcgtgtg ccagcgtcgt
    24361 ggcgaatcgt ctgaagcgtc ttgagagccg taaagatcat aatagccaga gccagatcaa
    24421 cgaactggag tcattgctgt tggcgctgaa taacattgat gccagccagt ttagcaaata
    24481 ccagttgctg ctcgacacca tccgaaaaga cctcgcctgg aaagccaata acacggaaga
    24541 tcgcctggtg atcttcaccg aaagtattaa aacgctggag ttcctcgaac agcaactgcg
    24601 agcggatctg aaattgaaag atgaccagat cgccacgctg cgcggcgatc agggcgatac
    24661 ggtattgatg gaaaccgtag aagcctttgg taaaacgcag tcgccgttgc gtcttctggt
    24721 ttgttcggat gtggcatctg aagggatcaa cctgcatcac cttagccaca aaatgattca
    24781 ctttgatatt ccgtggtcgt tgatggtatt ccagcaacgt aacgggcgta ttgaccgcta
    24841 tgggcaaaaa catcagcccc aaatccgcta tctgcttacc gaggccagcg aaccacagat
    24901 caatggcgat atgcgcgtac tggaagtgtt gatcaacaaa gacgaacagg cgcagaagaa
    24961 cattggggac agttcggagt ttaccggcaa atttacccag gaagaggaag aagagcaggt
    25021 tgcagagttc atgatgcagg atgatggtgc cagcctgttt gatcaactgc tgaacagcaa
    25081 cgtctcggaa agcgccgaac acgatctgtt tggcgaaata tgcagtgcgg tttccagcga
    25141 tgcctcaatg gtcaccgaaa cagacaccag cttgtttgcc agcgaacagg cgtactgcga
    25201 aagagcattg ggttacctga aagccagtgg tcaaactatc cagtatgaaa ccttgcctga
    25261 taataccttg tcgctggtgg caccggagga gttacgccgc cgcttcaacc agctaccgcc
    25321 tgaaattgcc ccggagaact ggcagctcta tttaagtcag gataaaaccg tcatcaccga
    25381 cgcgattgcg cgcgctcgcg gtgagcaaca tgcctggccc gatgtgcagt atctctggca
    25441 aatcaacccg gtagtgcagt ggctggacga taaaatctct tcggcttttg gtcgtcatca
    25501 ggctccggtt atccggctgc catacctgct tgaacccgat gaagatcact tcattctttc
    25561 cgggttattc ccgaaccgta aatcacatcc gatggtgaac ccgtggatag tggtgagctt
    25621 taaccgtgaa tcgctaatcg gcagtcagcc tttcgctgag tttttacaac gtcatccgca
    25681 gttgagtaac aagctgacta acagcggcgg taaagatcgt aatcaccaac gccagcagga
    25741 tttactggaa gcggctattg ctcatgcccg ggaggtattc atccatgatc ggaatgcgtt
    25801 tgaaacgcac attaaccagc aactgaatga gcatctgcaa aagttggacg ttttgcgtgg
    25861 gcggcagttg agccaacttg agctggattt tgccgataac aaacagcaat tgtcagtcaa
    25921 gcagagccgt aaagagcaga gacaacgcga aatcgaacac aattttgaca gctatatcga
    25981 atggattgag gacaccatga cgactgaaaa agaaccctac attcaggtaa ttgctgttat
    26041 caccggagcg gagggttaat catggcgctg gtcggtatta ataacgaaaa cgaattttac
    26101 tctaaccact atttgggtga ggttttcacc agtgatatcc gcgatgtgct ggaaccctgg
    26161 atagcccagg aaaatgcagc gcgtgaagcg gagcgtgccg ctcgtgaaca gggcaaagac
    26221 gtggagccgg gataccgcgc tccgtggaac cagtttaaca gtctggcgac tgagtttttc
    26281 cgcaaacttg ccgagcacga aaaacagcgt cagatcccgc agcgtctggc cgatcaacgt
    26341 aatcgctggc agccattgtt aaaggcgctg ggctacgaaa ttacgccaca gatccaaatg
    26401 ctggatgacg atacaccgct gccggtactg gcgcgttaca acagcactga cggtagcccg
    26461 tggctgtgga ttgttgaagc acacgatcag gaagaaggaa cgctggatcc gctggcgctc
    26521 tccttactga ccgcgcaatt cccggcggat accgacaaac ataagcacga cagcctgcgc
    26581 aaaaaagcca acggtgaata tcgcagctgg caggatttgc tctctacggc ggtcttcacc
    26641 caaaatgaac cgccgcgttt tgtgctgctg ctcggtaacc gtcagctatt gttgttggac
    26701 cgtactaagt gggcgcaaaa ccgtctgcta cgttttgatt ttgaagagat tttaagccgt
    26761 cgtgaaacgg atacgctgaa agcgactgcg gtgttgttac ataaagattc tctgctgccg
    26821 ggcagtgggg caccttatct tgactcgctg gatgacaatt cgcacaaaca tgcgtttggt
    26881 gtttcggaag atctgaaata tgccctgcgc gaaagcattg agttgctggg caacgaagcg
    26941 atgcattatc tgatcgaccg tggcctggca aactatacag gtaatcgtgc ggtggacccg
    27001 gatgaactga gccgcgaatg tctgcgttac atgtaccgcc tgctgttcct gttctacatt
    27061 gaagcgcgcc cggagctggg ttatgcgcca atgaccgcca aaacctatct gcaaggttac
    27121 agcctggaaa cgttgcgcga tctggagatg atcccgctga ccagcgaaga agatcgcaac
    27181 gggcgctact tccacgacag cctgaatatg ctgtttaaac tggtgcgcga aggctacaac
    27241 ggcggcgtga aaatgcagag tgacctggag agcggcgacc ggatcaccat ccatagtcat
    27301 cagttcagcg tcccgcgtct ggaaagtcat ctgtttgatg ccaacaacac gcgcattctt
    27361 aaccgcgtgg tattccgtaa cgaaaccctg caacagatta tccaggcgat gtcgttaagc
    27421 cgcccggcca aagggcgctt taaccgccgc ggacgtattt cttatcgcca gttgggtatc
    27481 aaccagttgg gtgcggtgta tgaggcgctg ctctcctatc gcggattctt cgccagcgaa
    27541 gatctctacg aggtgaagaa agccggggaa gagtttaacg agctggagac gggttacttc
    27601 gtcagtaagg atgagattag caaataccac gaagacgaga aggtctacga gaaagacggc
    27661 agtctgcgca ttcaccgcaa aggcagcttt atctaccgta tggccgggcg cgaccgtgag
    27721 aaatctgcct cttattacac cccggaagtg ctgacccgct cactggttaa atatgccctg
    27781 aaagaactgt ttaaggagca gattgatccc attagcgatc cgcacgccaa agctgatgcc
    27841 atcttaaacc tcaccgtgtg cgaaccggcg atgggcagcg cggcgttcct taacgaagcc
    27901 atcaaccagc tggcggaagc gtatctgttc cacaagcagc aggcggaagg tcgccgtatt
    27961 ccgcaggatc gttacaccca ggagttacag cgggtgaaaa tgtacattgc cgacaacaac
    28021 gttttcggcg tggacttaaa cccggtggcg gtggaactgg cggaagtgtc gctgtggctg
    28081 aacgccatta gtggcgatgc gtttgtaccg tggtttggtt accagctgca ctgcggtaac
    28141 tcgctggtgg gcgcgcgccg tcaggtgttc aacaagagcg aactgaccta caaaaaagcc
    28201 aaagatccga gctggcttaa cagcgagccg gtcgaactgg cgatgaacac gccgcgtgaa
    28261 gagacgcaga ttttccactt cctgctgccc gacggcggta tggctaacta cagcgataaa
    28321 actgttaagc agcgttatcc ggatgacttc aaagcgctgg acagctggcg caaagagttt
    28381 attaaaagct ttgccgggca tgagattgct gatgtgcagc gtatcagcga aaaggtggaa
    28441 gcactgtgga acacctatcg ccagcaactt aaagcagaac gtctgaaaac cgccgacagc
    28501 tacccggtgt ggccggcaga aaacagcgag cagacgcgtt cttcgctgag cagtaaagat
    28561 gaaaccttta gcggtcgtct tgaagataac agcgcctacc agaagctgcg ttgggtaatg
    28621 gactactggt gcgcgctgtg gttctggccg atcgacaaag ccgatgagct accggatcgc
    28681 ggcacctggt tgtttgagat tgaaaccctg cttgacggga ttgtaatcac ggaaaaagtc
    28741 actgaagttg cggagcacac caccggcgat ctgtttgccg aagaaggcct gctgcgagaa
    28801 gagtcttcgc tgttttctgt tgctggtcgt ctgaaaaccg aggtgttgtt ccgtcatttg
    28861 ccgcgtctgg cgattgtcga tgccctgaga aagcagcacc gtttcttcca ctgggatctg
    28921 gagttctgcg acctgtttgc cgagcgcggc ggttttgacc tgatgctcgg aaacccgccg
    28981 tggctgaaag tggaatggca ggaagcgggc gtgctgggtg attacgagcc ggaatttgtg
    29041 ctgcgtaagc tgagcgcctc gaagctggca acgttgcgta ttgatacctt taaccagatc
    29101 ccggcgctgg aagcggcctg gcgcagcgag tatgaaggct gtgaagggat gcaaaacttc
    29161 ctgaatgcgc agcagaacta cccggtactg cgcggggtgc agaccaactt gtataaatgc
    29221 tttctgccgc aggcctggcg attaggggcg cagaaaggcg tggcaggttt cctgcacccg
    29281 gaagggattt atgatgaccc gaaaggcggg caattacgtg cggcggtata tccgaggctg
    29341 agggcgcatt ttcagtttca gaatgagtta aatttgtttg ttgaagttga tcaccatgcg
    29401 aagtttagca gcaatattta ttctgctagc cctagcacag tgggatttga acatatatct
    29461 aatttgtatg ctccgcaaac tattgatgca tgttttgaac attctggcag tggggacatt
    29521 cccggtctca aagacgagat tgagagcgag ggaaaattaa aagttgtatg gaacacatct
    29581 ggccaccgtt ctcgattaat aagtatcgcc actcatgagc tagaattatt tgctcgtcta
    29641 tatgacagcg aaggaacgcc agcctggcag gcacgtttgc cagccttaca tgctaaacaa
    29701 cttgttgctg tactggaaaa gtttgctaat cagccgaata gattaggtga tttgcagggg
    29761 cagtattttt caacggttat gttcgatgaa acatatgctc agagggatgg gacaatttta
    29821 cggcagactc aattccctca agattcatca caatgggtac tgtctggccc tcatttcttt
    29881 gttgggacgc cgttctacaa gactccgcgc gaaaactgta cgcttaacag cgattatgac
    29941 tgcctggact tgctaactct gcctgacgac tatctgccgc gcactaacta cattccggca
    30001 tgtgatgcac aggagtatgc aaaacgtact ccatgcgtta catggactga actggctgaa
    30061 gatgaaccga agaaggtaac agattattat cgcttagcta tcagagccat gttggctcaa
    30121 tcgggggaac gtacactaat tagtgctatt tatccgccag aaataagtca catgaacgca
    30181 gtacgttctt actgctatag ctcacagaat ctgttactcg aacattcagg tatgtgtttt
    30241 tctttacctt ttgattttat ttgtaaatct actggcaagg caaacttaca tcagatgctt
    30301 gatggtttct catacgtatt attcaatccg agacaaaagg cattattata ctgcttagta
    30361 ttatcattaa attctgtaaa tgatgtatat gctggccttt ggcaatcctg ctacacccca
    30421 gacttcaaca cccagcgttg gagccgcgat ctcccgcagc tcccccagga tttcttcgcc
    30481 aaactgaccc cagagtggca gcgtaactgc gctttacgct ctgactacag tcgtcgtcag
    30541 gcgctggtgg aaatcgacgt attggtggcg caggcgctgg ggttaactct cgaagagctg
    30601 cttaccattt atcgcgttca gttcccggtg atgcgccagt acgaagcgga tacctggtac
    30661 gatcaaaacg gtcgcattat ctttacccca agcaaagggc tggtgggcgt tggcttgcct
    30721 cgcaccgcgc gtaaagctga cctgaaaaac ggctttgtct ttaacgtcga cagcccggag
    30781 tggaccggcg gtgactgcac cgatcaagct atcggttggg atgatgtcaa acatcttaaa
    30841 accggtaccg tcagcgtcac ctttgatgat tatacccgca gcgacgaagg tgagcgccgt
    30901 accgtcacct ggcaggctcc gtttatcaag ccagatcgcg aagatgacta caaagtggcc
    30961 tgggcgttct ttgcacaaga taaggagagc gcctgatgtt gccgtccgtt gtcagtcggc
    31021 aggtcgccga cagcgttgcc gcctttttac gggcggcgtt cccgctaaac agcccgctgt
    31081 ttaacggtga gaataacgat aatgtctcaa tgctggagca atttctggcc cagccggaaa
    31141 cgctgctgaa aggaccatac ctttccgccc agttgccgtt tcgtaaaagt gacttaccgc
    31201 tgaatttttt ccctaatctg acgctgccgt tcccgccgca cgcgcatcag gcccgggcat
    31261 ttcagcgtct gggaagcgac gcgccacagc cgacgctggt cgcaaccggt accggctccg
    31321 gtaaaacaga atgttttatg ttcccgctgc tcaaccactg cgcgggcgcg tcacaagccg
    31381 gggttaaagc gattatcatc tacccgatga acgccctggc aaccgatcag gccagccgtt
    31441 tcgccaaaac catcgccagc gatccacaac tgcatggcaa ggtgaccgtc ggcctgtttg
    31501 tcggcgacag cgaaatcgaa cccagcaaaa aaatgtcggc agaaaaggtc attacctgta
    31561 agcacacgct gcgcgaaagc ccgccggaca tcctgttgac caactacaaa atgctcgact
    31621 acctgctgat gcgccctggc gatcagccgc tctggcgtta caaccagccc ggttcattgc
    31681 gttacctggt cgtggatgaa ttgcatacgt ttgatggcgc gcaagggtcg gatctggcct
    31741 gtctggtgcg acgtttgaag catcacatag gtgtggataa ccaacgattc gcctgcgtcg
    31801 ggacatcagc taccgtcggc gacgaactgg ggcaactgct cgattacgca aaaacaatct
    31861 ttgaccagcc gtttaccgat gacgccgtta ttcgcgaaga tcgctacacg gcagcggagt
    31921 acttgcagaa ttacgccatt gcgtacagtc agtatccggg gccggaggtc gcttcggccc
    31981 tggacccgca gagttacgct accccggtag cgtatcttaa cgggcaaatc cctttgtggt
    32041 tccccgacag cgacttgcaa ctcccggacg atctggagag cgacgacggg cgtgaaaagc
    32101 gtattgcgct ggggtcactg ttacgccgcc acagcgtgat gcatgtctta ctccatgatc
    32161 tgcaaggcgg gatcctctct gaacgagagt gtctggaaaa tctccaggtc atgctggcag
    32221 aaagcgccga ccatgcccga cgcgtgctgc aaagcattct ggcactgatc gcgcaggcac
    32281 gtctcgaagt accggaaacg gaacacgatc ggcaaaaacg tctccaggcg aagaaagcgc
    32341 gtccggtgct gccgtttgtg caactgcgct cccagttgtg gcttcgtgag atgcgtcgtc
    32401 tggtcgccag cgtggcgaaa acgccccgac tggtgtttgc cgatgatgtg gcggtggaag
    32461 atcgcgaaca ctatttgccg gttatccact gccgtgactg ccacgcaact ggctgggcca
    32521 gcctgcgtca tgggcaaagc gttcagttgg aaacagacct cgacggcatt tatcgccagt
    32581 tctttgaaaa agggcgctcc atcgtccttg ctttcccgga taacaacgaa aaagcggtca
    32641 gtggcgcaca ccagaaactg tgtcctacct gtctgaaact gaaccgacag tccagcgtcc
    32701 agtgtggaca ttgcggtcat acggaactgt tgcaggtact gatcccggat atgctcaaag
    32761 agcgtaagga cgagctggag tttgctaatg aatgcccgta ctgcaacagc aaacagggga
    32821 tttcgattct ggggtcacgg gctgccagtc tgtcggcggt gatgattcac cagctttatg
    32881 gcagccagtt caacgaacat aaaaagctga ttacgttttc tgactcggta caagacgccg
    32941 cgcatcgtgc cagcttcttt actgcccgta cctggccgct gatgattcgt gggcaaatct
    33001 ccagtgttct tagcgaaaaa gagacattac cgctggatgc gttcgcggcg cgtgtttgcc
    33061 aacaaacgcg tgaacgttca ccggacgatg ctcattttgc agcaaacgtc atcgcaccaa
    33121 atatgcagtg gctgaatgat tatcagacgc tgaaaaatga aggcaaactg gcggagaact
    33181 ccgatctgcc ggagctggtt tccctgcgcc tcgactggga ggtgtacagc gaatttaccc
    33241 tgcgcgcgcg tatcggacgt acgctggaag ccaccggtta tgccgttgcc ggagtggata
    33301 aagatcgctt caatccggca attaccgccc tgcatcaaca gctttgtgaa gagctgggtg
    33361 atgagatgtc tggtgtgagc gtcgagcagg tagcgcacct ggcgctgggg gttatctggt
    33421 atcaacgcca ggccggggcc gttatgcatc cggcgttcga aagttatcgc cgtgatggca
    33481 cgacctttat gatgacgcaa aaagagcgta cctcgcgcta tatgccttct atcggtccga
    33541 aaacgcgtaa accggcttat gcgtcgtttg aaaaaattaa tggtttccat cgcctgattg
    33601 gcgcgaagtc taaccccagt tggtatcagc attggatcaa tcgcacgctg agtaacggca
    33661 ataatctgtt tatcagtagc gttgcggaaa cagtgttacg acgcctgttt acggcgttga
    33721 aaatagccgg agtagtaaaa gattttgaca ccaaagggcg tgaagcctgg ggactggtgc
    33781 cttcggcgct ggtggtgagc cgtgacgtcg ttcagcttaa ttgctcatgt tgccgtgagg
    33841 ttgtccgggc tccggccgat caaggctggc agtggcgtaa tgcgccttgt ctctctttac
    33901 gttgtgaagg tcgttaccag gaagtcgaca atacggcgac ctggcgttgg gagaacatgg
    33961 acatcgcgcg cgtacagggc gcagaacaca cggggctgct tagccgtgaa gatcgcgaag
    34021 tcaccgaaaa atcgttctac cgcggcaacc aaccgtggaa tatcaacctg ttgtcggcaa
    34081 caccgacgct ggagatgggt attgacgttg gggatctttc caccgtttta ctctgttctg
    34141 tcccacctgc tcaggccaac tacttgcagc gtattggtcg cgccggtcgt aaagatggta
    34201 acgcgttaaa cattaccgtg gcagaaggga acccgcacga tcagtttttc tttgaagaac
    34261 cactggagat gatgcagggc caggtgcagg ctcctggcgt cttccttaat gcaacggcta
    34321 ttctcgaacg ccagctggca gctttttgta tggataactg ggtgaaaacc ggtgtaccag
    34381 aatccgctat ctgtaaaaac gtcaaacaga tgctggatga gctggagttc gggcgtaaat
    34441 cgggttttcc gtataactta ctgcgatata tcgaacagca tcatgcggag attgctgcgc
    34501 aatttacggc aatcttcccc gatcttgccg cagaaactcg ccaacagctg ttgtcttatc
    34561 ttcagggcgc gccaggacaa cgctcgctgg tgcagcgtat cgaagaagcg ttaaaactgc
    34621 tggtggaaga tcgcaaatct ttgcgctcac gcatcgataa actcaagcgc agtatcgata
    34681 aactggagaa cgcgccgcgc gatcaaaact ttgatagcga tatgcgcgag ttaacttcgg
    34741 aacggcaggc gctgatggcg ctggtcaatc aaatcaacaa taagcaaacg ctgaatttcc
    34801 tcacggatga aggtctgctg cctaactacg ccttcccgga agccggtatt acgttacgtt
    34861 ctgtgctctg gcggcgtaaa gaaggcgggg aagcacgtga atatcaaaac accactttcg
    34921 aatatgaacg cccggcatca acggcgctgg ctgagctggc tccgctgaat aatttctatg
    34981 ccggtgggca taaagttgaa attgagcaga tagacctgaa ggtctctgag ccggagaact
    35041 ggcgtatctg tagccactgt aactacagcg aaaatatcga ccagacgggc gaccagcata
    35101 aatattgccc gaaatgtggc acaccggggt gggccgatgc cggacaaaaa accacgctgc
    35161 tgaaacttcg ccaggtttat gcccgcgcca gcgcacgtga tagccagatc agcgacgaga
    35221 gcgacagccg tgaacctgct ttcttccagc gtcagctatt ggttagtttc gaaaaagaag
    35281 atgtatcggc ggcatacgcg attgaggaag gcgaagttcc gtttggtttt gaatttttaa
    35341 gcaaagtcac cctgcgggat atcaacttcg gcaaaatggc ggatgatgcc aatgagttga
    35401 tgattgccgg agaagcgaag aagcgaaccg ggtttaaagt ttgcctcggg tgcggcatgg
    35461 tgcagcgtcc tcgcgatcat gaaccacgtc atgatttgag ctgcaaatat cgcacggaac
    35521 cggaaaaggc gaagttcgaa gattatctct acctctatcg ccagctggag tcagaagcgt
    35581 tgcgtattct gctcccggta agcagttaca gtaatgaccg tgtcgtggag gcttctcttg
    35641 gggcggcgat ccagctcggt cttaaacatt acttcaaggg gaatgtcgat cacctgaaag
    35701 gcgttgtgta ccgcgagccg gaaaatgaag gcgaatcctg gcggcagtat ctggtgattt
    35761 acgataccgt cccggggggg actggttctc ttaaagaact gatgcgcact cccgacaatt
    35821 tgctgaaatt acttgaactg gcctatcagg caatagtgga atgcagttgc aacaatgata
    35881 cgcataaaga tggttgttat cgttgcgttt acgcttaccg tgaccgtggg cgcatgaagt
    35941 atgtctcccg cgatcaggcg cgtctcttgc tggcgaaaat tttgcaggca agagattcga
    36001 tacgggtaat tgattccatc aaaaacattt ccctcgaagc catgatgggc agtgagctgg
    36061 aaaaacggtt tattagctgc ctgcaagaaa ataaaaacct gattgtcagt cgcagttacg
    36121 cgcatcaggg cgctgggtgg attattaaca cccgcagcga accgatgatg agctggcatc
    36181 ttaaagctca ggtggattta ggcgctaaag aggcggtggg gatcccttgt cgcccggatt
    36241 acgtgctata tccgcttatg cagtctgatg ccattaaacc ggtggctatt ttccttgatg
    36301 gctttgcgtt ccacaaaaat agcgtcgcag aggatgtgca aaaacgccag gcgattcggg
    36361 atagcggtaa tttctgggtg tggacagtca cgtgggccga tctcaaagag ccagggctta
    36421 aacatgttca ggatgtcctg ggacttgggc ataacccgga tatgaaacag cctaagtttt
    36481 ataacctgtt tcatgatacc aactttgcgg cccttgaagc gtcgttccgg gaacgcaaca
    36541 gttttgcact gctgctggat tatctcgccg atccgggcgg aagaacgcag ttatggcaga
    36601 gaatggctgc tgcacatgcc tgggtgtggt tggatgtgaa gaagtcacag gacgcgggaa
    36661 cgaaacaaaa gtatgcctat gaaatgcagg aaaacgcgcc agcttggcgg ctagcagagc
    36721 ttttgccaga cgaaccgttt gtctttggtg gtttactcga tagctgtaac tcatcgcagc
    36781 aatttattga gctggcttcg gtgttaccac aacaagccat taaaccgacg accagcgtcg
    36841 cacaaatgcg cagctggctg cgtttgcata tctgttttga cgatcgctat acgcaggatg
    36901 acggttatga agcgggactc aatggcttct ggcgactggt aaacctgtta cagttcctgc
    36961 cggatatgac ctttaccagc cgtaaagccg tgcatttacc gcaggaaagc gttgacacta
    37021 aagtttcggc aacgccagag gcagtaccag agactgatga gagttgggct gagattattg
    37081 agtttggctt attaagcgct gaagagatcg cgttgcttca gtctcattct ctctctgcgc
    37141 cgactctcgg ctacgaattg caaaatgatg aaggtgaaat tattgcggaa gccgatctcg
    37201 cctggccatc acaaaaatgg gcattgatta ttgataatca agaatttatt ccattattca
    37261 caaccagggg ttggcacgtt gcattcgggc caattgacga aaatacattg caacatctgt
    37321 ccggaggtga caaatgacgg tgcaaacgcc caaggtggca ctttctgatg gttttctggg
    37381 cgcattcgcg cggatcccaa aggcacagca aaaaaaggtg caggaattca tctccaaatt
    37441 ccgtcaggac ccgaccagta atggacttaa ctatgaaaag atccacgatg cgcgcagtaa
    37501 aaatgtccat tcggtgcgaa tcgatcaaac ttatcgcggc attgttctga aacctgaaca
    37561 gggtgccctg tatatgctga tgtgggtgga taaacacgac gaagcatatg actgggcgcg
    37621 gcggcatgat tgttcaattc acccggttac cggcgcgatt caggtcattg atattagcta
    37681 tatcaaaccg gcagcggaaa cagtggttga taaaccgaaa ctcttcgctg cttacagcgc
    37741 ggaacagatt ctggctctgg gtgtgccgcc tgtctttatt gatcaggtga tggcgcttac
    37801 cgatgaagcc gggcttaatc aacttgaaag cataatgcca gcagaagcct gggaacctct
    37861 gcactggctg gcagaagggc tggattacca ggaagtgctg gaagagttta acgatcatcg
    37921 tgatgagcct gttgatacca acgactttat ggaggcgatt gaacgaagta aacgtcgctt
    37981 ccatgtcgtt gaaaatgaac aggagctgtt gcagatgctt aatgcgcctt tagaaaaatg
    38041 gcgtgtattt ctgcatccga gccagcgaaa attggtggag tcgcccgcta atggtccggt
    38101 acgagtactg ggcggtgcag gtacagggaa aacggttgtt gcgatgcacc gtgcgcggtg
    38161 gttatctcag cgtctggcag ataaacccgg taagaaagta cttttcacga cctttacgcg
    38221 taacctggcg gcagatattc gcgctaatct ccagcggttg tgtacgcgag aagagatggc
    38281 gcgtattgac gtcgttaaca ttgatgcctg gatgagcgac caactaaaac gccacggtta
    38341 tgatttccgc gtggtatacg acagcgatga agggcgacgc aaatgctgga actatgcctt
    38401 gcagcaggca cctgccgaac tggggctacc tgataatttc tatgctgaag agtggcaacg
    38461 cgttgtccag ccaaatgcgg tttacacccg ggaggaatac ctcaaagtca gccgggttgg
    38521 acgtggtaca gcgttatccc gtattcaacg ggcgaaaatc tggccagtct ttgaagaatt
    38581 cagagcacaa atggcgcgtg ccaaactgcg cgaaatgggc gatgccatgc atgaggctat
    38641 tgttctgttt aaagaaaagc aggtccaact accttatagc agtattgtgg tcgatgaagc
    38701 tcaagatatc ggtgccccgg catttactct tattcgcagc ctggttcccg agtcgcccaa
    38761 cgatctgttt attgtcgggg atgggcatca gcgtatttat cgcaataagg tcgttcttgg
    38821 tcagtgtggt attaacgtcc gtggtcgtcg cagtaaaaaa ttgaagatta actatcggac
    38881 cacagaagag acgcgtcaat ttgctgttgg gctgttaact ggtgtgaagg ttgataacct
    38941 cgatggcgaa gcggatacca gtaatgatta tttgtcatta ctgcacgggg aaaagcccat
    39001 gattacgcat gcggcagatt tcaaagaaga ggctgcaacg ctcgtcaagc aaattcaggc
    39061 attgctggcg aatcaggttc gttctcagga tatctgcatt actgcgcgta ctaaacatgc
    39121 ttgtgaccgc tacgcatcag cactgaatga cgccggaatt gagacattta atttaggcaa
    39181 tgatagcggt gacagcgatg cacgccctgg cgtgcgggtt gcaacgatgc accgtattaa
    39241 agggcttgag ttccagtatg tgttcctggt ggggattaac gaaggcgttg tgccggaaat
    39301 aaaagcatta gcatccgatg atcccgttga acagcgtgat gcattattta atgagcgcgc
    39361 gttactgcat gtggctgcaa cccgtgccgt aaaagggctg tttgtttcat catctggtgt
    39421 ccctagtccg ctattagtgg ctgactgata atatgtccaa aacaggccag gagccgaaac
    39481 gctcctggtc tatataaccg ttctggcaac tggcgatatt tatattgctt agctcccaac
    39541 agccagacaa tatcaactta gaaattatat tcgcttatgc ggtttgcata ttgcacagta
    39601 caacctgttt tctctatatc taacgtaacg tcaggatgtg gatgattttt ggatggggtg
    39661 acacactgga caaatatagc tggcacgaaa aggctggcat ttccagctga ccaatagtct
    39721 ctggaaccat gatgatgata aaataaatat cttaagtttt aaacgtatga tgccaggatt
    39781 accagaatta taaacgaata tattagttct tttctatttg ttcaggaggg gtataaaaat
    39841 tgcaattgtt aatagtatcg tcaggacgat aaaaagataa tcatgacaaa ggcaattgaa
    39901 ctaaaatgat tgacatttta caggtggttt gtcagtgatt tcttgttatt cataatttat
    39961 tttccgt
//
LOCUS       DAZFKY010000021        38108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFKY010000021 DAZFKY010000000
VERSION     DAZFKY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            108..719
                     /gene="ruvA"
                     /locus_tag="ACHKCL_000997"
     CDS             108..719
                     /gene="ruvA"
                     /locus_tag="ACHKCL_000997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5762014.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            728..1738
                     /gene="ruvB"
                     /locus_tag="ACHKCL_000998"
     CDS             728..1738
                     /gene="ruvB"
                     /locus_tag="ACHKCL_000998"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5762015.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ACHKCL_000999"
     CDS             complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ACHKCL_000999"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5762016.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ACHKCL_001000"
     CDS             complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ACHKCL_001000"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5762017.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3600..4532
                     /gene="znuA"
                     /locus_tag="ACHKCL_001001"
     CDS             3600..4532
                     /gene="znuA"
                     /locus_tag="ACHKCL_001001"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5762018.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4548..5870
                     /gene="mepM"
                     /locus_tag="ACHKCL_001002"
     CDS             4548..5870
                     /gene="mepM"
                     /locus_tag="ACHKCL_001002"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5762019.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5990..6961
                     /gene="lpxM"
                     /locus_tag="ACHKCL_001003"
                     /gene_synonym="msbB"
     CDS             5990..6961
                     /gene="lpxM"
                     /locus_tag="ACHKCL_001003"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5762020.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ACHKCL_001004"
     CDS             complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ACHKCL_001004"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5762021.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ACHKCL_001005"
                     /gene_synonym="yebK"
     CDS             complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ACHKCL_001005"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5762022.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9869..11344
                     /gene="zwf"
                     /locus_tag="ACHKCL_001006"
     CDS             9869..11344
                     /gene="zwf"
                     /locus_tag="ACHKCL_001006"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5762023.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11579..13390
                     /gene="edd"
                     /locus_tag="ACHKCL_001007"
     CDS             11579..13390
                     /gene="edd"
                     /locus_tag="ACHKCL_001007"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5762024.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13427..14068
                     /gene="kdgA"
                     /locus_tag="ACHKCL_001008"
                     /gene_synonym="eda"
     CDS             13427..14068
                     /gene="kdgA"
                     /locus_tag="ACHKCL_001008"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5762025.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ACHKCL_001009"
     CDS             complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ACHKCL_001009"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5762026.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15436..15726
                     /gene="yebG"
                     /locus_tag="ACHKCL_001010"
     CDS             15436..15726
                     /gene="yebG"
                     /locus_tag="ACHKCL_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5762027.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15793..16149
                     /gene="yebF"
                     /locus_tag="ACHKCL_001011"
     CDS             15793..16149
                     /gene="yebF"
                     /locus_tag="ACHKCL_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5762028.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16476..17135
                     /gene="yebE"
                     /locus_tag="ACHKCL_001012"
     CDS             16476..17135
                     /gene="yebE"
                     /locus_tag="ACHKCL_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFO5762029.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17344..19404
                     /gene="ptrB"
                     /locus_tag="ACHKCL_001013"
     CDS             17344..19404
                     /gene="ptrB"
                     /locus_tag="ACHKCL_001013"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5762030.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ACHKCL_001014"
     CDS             complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ACHKCL_001014"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5762031.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ACHKCL_001015"
     CDS             complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ACHKCL_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5762032.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ACHKCL_001016"
     CDS             complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ACHKCL_001016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5762033.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21214..21588
                     /gene="yobA"
                     /locus_tag="ACHKCL_001017"
     CDS             21214..21588
                     /gene="yobA"
                     /locus_tag="ACHKCL_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5762034.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21592..22464
                     /gene="copD"
                     /locus_tag="ACHKCL_001018"
     CDS             21592..22464
                     /gene="copD"
                     /locus_tag="ACHKCL_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5762035.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22477..22818
                     /gene="yebY"
                     /locus_tag="ACHKCL_001019"
     CDS             22477..22818
                     /gene="yebY"
                     /locus_tag="ACHKCL_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5762036.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23214..23870
                     /gene="pphA"
                     /locus_tag="ACHKCL_001020"
     CDS             23214..23870
                     /gene="pphA"
                     /locus_tag="ACHKCL_001020"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5762037.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23871..24062)
                     /gene="yebW"
                     /locus_tag="ACHKCL_001021"
     CDS             complement(23871..24062)
                     /gene="yebW"
                     /locus_tag="ACHKCL_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5762038.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24167..24403)
                     /gene="yebV"
                     /locus_tag="ACHKCL_001022"
     CDS             complement(24167..24403)
                     /gene="yebV"
                     /locus_tag="ACHKCL_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5762039.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24521..25960)
                     /gene="rsmF"
                     /locus_tag="ACHKCL_001023"
     CDS             complement(24521..25960)
                     /gene="rsmF"
                     /locus_tag="ACHKCL_001023"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5762040.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26041..28674)
                     /gene="yebT"
                     /locus_tag="ACHKCL_001024"
     CDS             complement(26041..28674)
                     /gene="yebT"
                     /locus_tag="ACHKCL_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5762041.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28643..29926)
                     /gene="yebS"
                     /locus_tag="ACHKCL_001025"
     CDS             complement(28643..29926)
                     /gene="yebS"
                     /locus_tag="ACHKCL_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5762042.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30056..30553
                     /gene="msrC"
                     /locus_tag="ACHKCL_001026"
     CDS             30056..30553
                     /gene="msrC"
                     /locus_tag="ACHKCL_001026"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5762043.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30650..31348
                     /gene="proQ"
                     /locus_tag="ACHKCL_001027"
     CDS             30650..31348
                     /gene="proQ"
                     /locus_tag="ACHKCL_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5762044.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31368..33416
                     /gene="prc"
                     /locus_tag="ACHKCL_001028"
     CDS             31368..33416
                     /gene="prc"
                     /locus_tag="ACHKCL_001028"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5762045.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33608..34489
                     /gene="htpX"
                     /locus_tag="ACHKCL_001029"
     CDS             33608..34489
                     /gene="htpX"
                     /locus_tag="ACHKCL_001029"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5762046.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34535..35908)
                     /gene="yebQ"
                     /locus_tag="ACHKCL_001030"
     CDS             complement(34535..35908)
                     /gene="yebQ"
                     /locus_tag="ACHKCL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762047.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36085..36876
                     /gene="kdgR"
                     /locus_tag="ACHKCL_001031"
     CDS             36085..36876
                     /gene="kdgR"
                     /locus_tag="ACHKCL_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5762048.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37020..37259)
                     /gene="yobH"
                     /locus_tag="ACHKCL_001032"
     CDS             complement(37020..37259)
                     /gene="yobH"
                     /locus_tag="ACHKCL_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5762049.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37419..37562
                     /gene="mgrB"
                     /locus_tag="ACHKCL_001033"
     CDS             37419..37562
                     /gene="mgrB"
                     /locus_tag="ACHKCL_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5762050.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37637..37924
                     /gene="yebO"
                     /locus_tag="ACHKCL_001034"
     CDS             37637..37924
                     /gene="yebO"
                     /locus_tag="ACHKCL_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5762051.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9762 a         9381 c         9826 g         9139 t
ORIGIN      
        1 cagttcatcg agacacctcg caagtttttt tcatccttcg ctggatatct atccagcatt
       61 tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac
      121 tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag
      181 gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag
      241 cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca
      301 ataaacaaga gcgcacgttg ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt
      361 tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg
      421 aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga
      481 ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg ccagccgccg
      541 acctggtact cacgtctcct gccagcccgg cgaccgacga tgctgaacaa gaagcggttg
      601 ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg
      661 ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag
      721 gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt
      781 agcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg
      841 ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt
      901 gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga
      961 aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc
     1021 tgcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgttt
     1081 atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat
     1141 gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat
     1201 tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt
     1261 gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagcgcacg
     1321 ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg
     1381 tacgccgcgc attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca
     1441 cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga atgtcgatgc
     1501 tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg
     1561 tgggccggta ggtctggata accttgcggc agccattggc gaagaacgtg aaaccattga
     1621 ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgtgggcg
     1681 tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt
     1741 cggattggtc gctcgcaccg catccgacat tggcagtgtg cctgatgcga cgctattcgc
     1801 gtcttatcag gcatatacca gagatagcga accgtaggtc ggaaaaggcg ctcgcgccgc
     1861 atccgacatt ggcagtgtgc ctgatgcgac gctgttcgcg tcttaccagg cctaccagag
     1921 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     1981 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     2041 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgt cagttactgc
     2101 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     2161 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     2221 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactc gctgtaattt
     2281 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     2341 ccagaacaaa atagccacca cgatgaccac gccaattgca atagagatga gatcttctgg
     2401 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     2461 cagactaacg accaccaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     2521 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     2581 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     2641 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     2701 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     2761 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     2821 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     2881 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtgatgattc
     2941 aggcacagca cttcatcggt ttttgccatc accagatgaa gatcgtgaga aaccattaaa
     3001 acgccacaat ccagttcacg acgcagttgg tcaataaggt catataacgc cacctggcca
     3061 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggcg atttaacaat
     3121 gctcgcgcta acagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     3181 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     3241 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     3301 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     3361 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     3421 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     3481 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     3541 tagtcatgaa atgttataat atcacacttc tcatgttcat tacgatgatt ggtcgcatta
     3601 tgttacataa aaaaacgctt cttttcgcag cattattcgc cgctctctgg ggcggtgcaa
     3661 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     3721 ccattgctga tggggtaaca gaaacggagg ttttacttcc tgacggcgct tcagaacatg
     3781 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     3841 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     3901 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attaacggcg
     3961 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     4021 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     4081 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     4141 aggcacaatt agcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     4201 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     4261 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     4321 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     4381 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatccct
     4441 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     4501 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     4561 cccgctctgt cgccctggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     4621 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgcgatg
     4681 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     4741 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     4801 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     4861 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caactggctg
     4921 cggccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     4981 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     5041 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     5101 acaatctgct gaaaggtacc gtcgggggga gctttgttgc cagcgccaga aacgccggtt
     5161 tgaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
     5221 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
     5281 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcta
     5341 tccgcgctga agatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
     5401 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgtacta
     5461 atccggtgac cggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccgcagggta
     5521 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
     5581 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
     5641 agattctggt gaaaccgggg cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
     5701 ataccggacg ttcaaccggg ccgcatctgc actatgaagt atggataaac cagcaggccg
     5761 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
     5821 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
     5881 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgcgcc
     5941 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
     6001 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
     6061 gggagcatgg ctgggcgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
     6121 tgatcccatt ctggcacggc tgggacgtat tgccggacga ctgggaaaaa gctcacgccg
     6181 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
     6241 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
     6301 aatacgcggg ccggagaaaa ttcagccgcg cgttgactgg caagggctgg agatcatcga
     6361 agagatgcgg cgtaataacg agaaagttat ctttctagtg ccgcacggtt gggccgtcga
     6421 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
     6481 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
     6541 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
     6601 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
     6661 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtgtgcc gtgcgcgcgt
     6721 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
     6781 accgatggat gatctgttag aggcggatga tcatacgatt gcgcggcgga tgaatgaaga
     6841 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
     6901 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
     6961 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
     7021 attcgaaatc tgttgaacta tcattgaact gtaggccgga tgtggcgttt tcgccgcatc
     7081 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
     7141 atcacgtcgc cctgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
     7201 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
     7261 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
     7321 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
     7381 atggtaatga tcgccgtaac gcctttcagg tggttagctg cgtacattgc tgacatggca
     7441 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
     7501 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
     7561 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
     7621 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
     7681 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
     7741 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
     7801 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
     7861 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
     7921 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
     7981 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
     8041 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
     8101 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
     8161 tcaccaggca cgacgtcagc aggcaggcct ttgtagttga taccgacttt ttctttgtcg
     8221 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
     8281 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
     8341 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
     8401 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
     8461 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
     8521 agccttctgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
     8581 atccgaatga aataacgccg cgatggcgat aaaataatct cattcggatg tcgtataaga
     8641 ttagaacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
     8701 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
     8761 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
     8821 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
     8881 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atagcgtcgt tttcgcgtgc
     8941 cagctgcgcc agctcgacca gattttttgt tcttccggtg tgagaaatca gcaccaccac
     9001 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acaatatcat cggagtacac
     9061 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggctgaaga
     9121 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
     9181 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
     9241 tttccctgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
     9301 gccattcgcc agactctgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
     9361 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagtg ccagagcagc
     9421 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
     9481 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
     9541 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
     9601 attacgcctg tgtgctgtgt taatgacaaa agcagataaa aaagttgtta ttttttttca
     9661 taacatgatc agtgtcagat ttttacccaa tggaaaacga tgattttttt atcagttttg
     9721 ccgcactttg cgcgctttta ccgtaatcgc acgggtggat aagcgtttac agttttcgca
     9781 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
     9841 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggtctgtga
     9901 cttggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
     9961 tcaactggaa aaagccggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    10021 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    10081 agaaaccatt gatgaaggtt tatgggacac cctgagcgca cgtctggatt tttgtaatct
    10141 cgatgtcaat gacactgctg cattcagccg tctcggcgcg atgctggatc aaaaaaatcg
    10201 tatcaccatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    10261 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    10321 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    10381 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    10441 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    10501 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    10561 gatgcgcgac atgatccaga accacctgct gcaaattctt tgcatgattg cgatgtctcc
    10621 gccgtctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtctct
    10681 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    10741 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaagagcag
    10801 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    10861 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    10921 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    10981 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    11041 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    11101 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    11161 tattcaggca ctgtttgtac gtcgcgatga agtggaagaa gcctggaaat gggtagactc
    11221 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    11281 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    11341 gtaatatctg cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    11401 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    11461 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    11521 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    11581 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    11641 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    11701 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag cctctttgaa
    11761 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgctca
    11821 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    11881 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    11941 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggctg tcgggctgtc
    12001 ccataacatg tttgatggtg ctctgttcct cggggtgtgc gacaagattg tcccgggtct
    12061 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    12121 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    12181 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    12241 tactttctac ggtactgcca acaccaacca gatggtggtg gagtttatgg ggatgcagtt
    12301 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    12361 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    12421 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    12481 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    12541 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    12601 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    12661 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    12721 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    12781 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    12841 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    12901 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    12961 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    13021 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    13081 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    13141 agctatccac gtaacaccag aagcttacga tggcgggctg ctggcaaaag tgcgcgacgg
    13201 ggacatcatt cgtgtgaatg gacagacagg cgaactgacg ctgctggtag acgaagcgga
    13261 attggctgct cgcgaaccac acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    13321 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    13381 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    13441 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    13501 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    13561 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    13621 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    13681 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    13741 cgaagggact attccgctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    13801 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    13861 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    13921 tccggctaac taccgtgact acctggcgct gaaaagcgtg ctgtgcatcg gtggttcctg
    13981 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    14041 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatcgagg atcgggcatt
    14101 tttgtagcgt ttatttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    14161 gcgcgttcaa tggcgtcaac aacactctct gcagtagcca gtgccacccc cagacgacgg
    14221 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    14281 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    14341 gcaggaccat actgacggat cccgccaacc ggaagtccga ggaaggcacg tacatgcagg
    14401 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    14461 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    14521 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    14581 gggctcattt gctgtggttg ccaggattca cagtagtcgc catcttcctg gcgatgacct
    14641 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    14701 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    14761 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    14821 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    14881 aaaaggcttt cgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    14941 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    15001 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    15061 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    15121 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    15181 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    15241 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    15301 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    15361 tttattgctg gtgttgatct ctcaaccgaa aagaaatata ctgtatagaa tcacagttat
    15421 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    15481 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    15541 gatctgcttg acacctggct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    15601 gccctttcgc tatggctggc agaacaaaaa gatgtgctga gcaccattct gaaaaccggc
    15661 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    15721 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    15781 atggagaaaa acatgaaaaa aagaggggcg tttttagggc tgttgttggt ttctgcctgc
    15841 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    15901 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    15961 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc atgggtcagt
    16021 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt acgtggtaaa
    16081 agtgccgata ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag
    16141 cggcgttaat gggttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    16201 tccgccccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    16261 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    16321 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    16381 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atcctctatg
    16441 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    16501 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    16561 aacttttagt gccgggcgca ttaggcggac tggctggact gctggtcgca aataaatcag
    16621 cacgtaaact tcttacgaaa tatggcacca acgcgttgct gattggcggc ggagcggtag
    16681 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    16741 cgcagtttgg ctcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    16801 tagtctttgc cgctaaaagt gatggtcata ttgatgccaa agaacgtgcg gcaatcgacc
    16861 agcaattgcg tgaagccggc gtggaagagc aggggcgggt actcattgag caggcaatcg
    16921 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    16981 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    17041 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    17101 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    17161 tgactatttc gggctatcca gaacagcaca gccatttgaa caggctgatt ttctctgtct
    17221 gcacaacatt cgatattcgc gccattacct gtcatcatct aagcaatgac tgccctgttt
    17281 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttcaaccag aaagaacaat
    17341 aacatgctac caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc
    17401 atcgataatt actactggct gcgggacgat acgcgttctc agccggaagt cctggactac
    17461 ctgcaacaag aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat
    17521 cgcatcttaa aggaaatcat cgaccgcatt ccgcaacgag aagtttctgc gccctacatc
    17581 aaaaatggct accgctaccg gcatatttat gaaccaggct gtgaatatgc tatctaccag
    17641 cgtcaatcgg cgttcagtga agagtgggac gagtgggaaa tattgctcga tgccaacaag
    17701 cgcgcggctc atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc
    17761 attatggcgc tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat
    17821 ctggaaacag gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg
    17881 gcaaatgact cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat
    17941 caggtctggc gtcacgccat cggtacgcca gcatcgcaag ataaactgat ttacgaagaa
    18001 aaagacgata cctattacgt cagcctgcat aaaacgacct caaagcacta tgtagtcatt
    18061 catttggcca gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggcagatgcc
    18121 gagccgtttg tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag
    18181 catcgttttt atctgcgttc caaccgccac ggcaaaaact ttggcttata ccgtacccgt
    18241 atgcgtgatg agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa
    18301 gggtttacgc tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt
    18361 ttgcgccaaa ttaaccgcaa gacccgggaa gtcattggta ttgcctttga tgatccagcc
    18421 tatgtgacct ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat
    18481 tcttccatga ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt
    18541 gtattaaaac aaacagaagt tcctggtttt gatgcggcga attaccgcag tgaacacctg
    18601 tggatagtcg cccgtgatgg tgtcgaagtt ccggtttcgt tggtctacca tcgcaatcat
    18661 tttcgcaaag gacacaaccc gctgctggtg tatggctacg gttcttacgg cgcaagtatt
    18721 gatgccgatt tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt
    18781 gtccatgttc gcggcggcgg tgagctgggg caacaatggt acgaagacgg taaatttctg
    18841 aagaagaaaa atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc
    18901 tatggctctc cttcgctttg ttatgcgatg ggcgggagtg cggggggcat gttgatgggc
    18961 gttgcaatta atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt
    19021 gatgttgtaa caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag
    19081 tgggggaatc cgcaggatct gcaatattac gagtatatga aaagctacag cccgtatgac
    19141 aacgtcaccg cacaggctta tccacattta ctggtaacga ccggtttaca cgattctcag
    19201 gtgcaatatt gggaaccggc aaaatgggtc gctaagttgc gcgagctgaa aaccgataac
    19261 catcttttat tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggtcgcttt
    19321 aaatcgtacg aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca
    19381 ttacccgcta ggcctgcgga ttaggtattt tccagataat gtttcagtgt taaacgcagc
    19441 tccgggctca tgctgtccag gttattaaac aaccagcgca gatagcccgg atcgcgttcg
    19501 gcaacgtcgg aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac
    19561 ggacgtccgg ttatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg
    19621 ataagcaacg cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc
    19681 ggcgtctgta cattgagctt ccgtgtttta tataacgcca tattgctgta cttgatccct
    19741 ggccacaaac gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt
    19801 acgcggcggt caaagctggc gttatgcgcg acataccatt cactaccgta atagtgtggg
    19861 atcacatctt caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc
    19921 atcgcttgtg gactaatagg acgatcgggg tgcaccaggt ggctcatggg gttgacgatt
    19981 ttcccgtcaa tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt
    20041 tctgtatcga taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa
    20101 tgatatcgcc ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat
    20161 cggcgcgaac gatgagacga ccatgttcat cccatagtgc actgttacca cgggcatttg
    20221 ccatcagcac agcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgatgcca
    20281 gcaggtcggg ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat
    20341 ccataccttg cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg
    20401 caccatgact ctgatggtag atcccaggcg tttttcgcca cggtgcgaac accgcaattc
    20461 cacgaataaa gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgccagg
    20521 tcgttgcggc ataacaaagc gggtctaaca gtgagagatc gggtggggca ggaagggcac
    20581 gtcgcgaata atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt
    20641 gcctggcggc ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt
    20701 tacgaggctc atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa
    20761 aatgactgca aattatgggt gcggtttaga aaatactcat aatttagcag gcgttatgta
    20821 agaaagtgta actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag
    20881 attgaccgaa gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc
    20941 aggctgttca cgttcaaccg cttcagcgat cactggcata ttgtagcgtt ctttaaatgc
    21001 caccccggcc gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc
    21061 cagattcttc agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag
    21121 cctggatggc aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct
    21181 gtaaaaaagt tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc
    21241 tacgcgctgg caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat
    21301 ttaacgcatc agtatcctgc ggcaaacgcg caagtgacag ctgcaccgca ggcaatcact
    21361 ttaaacttct cggaaggtgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa
    21421 aacgaaaata ttaaaacatt accggcgaag cgaaatgagc aggaccaaaa gcgactgatt
    21481 gttccactgg ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg
    21541 gtggacgggc ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg
    21601 tttacctgga tcgcgctgcg atttatccat ttcacctcgc tgatgctggt ctttggcttc
    21661 gcaatgtacg gtgcctggct ggcaccctta acgattcgtc gcttgctgac gaagcgtttt
    21721 ctgcgtttac aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc
    21781 gttcagggcg ggttgatggg aacgggatgg aaagatgtat tttcgccaaa catttggcag
    21841 gctgttttgc aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta
    21901 acgttgatcg tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact
    21961 accgcgcaat ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaacg
    22021 gcgaaaatcc atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg
    22081 ggattactgc cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acaccaggct
    22141 attcaggcgc tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca
    22201 agcggcgtgc ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg
    22261 ggccaactct tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct
    22321 aatcgttatg ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt
    22381 gtgtggatga ctaaactcga atgggcgata ggtgcggtag tgctgctgat catcagcctg
    22441 cttgcgactc tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg
    22501 tttctgttac tcaccagttc tgcagcggcg ctggctgcac cgcaggtgat taccgtcagc
    22561 cgttttgaag tgggtaaaga caaatgggcg tttaatcgcg aagaggtgat gctgacttgc
    22621 cgaccgggta atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac
    22681 gatgtcgcac agaaggaagt tgccagtggg aagactaaag cccaacccat ttcggtgatt
    22741 cagattgatg atcccaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga
    22801 gctgaaaaac tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct
    22861 cagttctttc accagaacgg gcggttttta acatttaagc tgatgaccac cgcgcttttt
    22921 attgaccatt ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa
    22981 ggcaactaag cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt
    23041 ccctggaccg aatacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca
    23101 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
    23161 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
    23221 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
    23281 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
    23341 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
    23401 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
    23461 tggcgatgga tgcgctggca tcccagcaga tgtctttgtg gttgatgaat ggcggcgact
    23521 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
    23581 agcatttgcc ctttattctt gaagtacaca gccgcaccgg caaacatgtt attgctcatg
    23641 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtcttgt
    23701 ggagccgctc gcgattaagt gaacgccaaa aagggcaggg aattacaggt gctgatcatt
    23761 tctggtttgg tcatacaccg ttgcgccatc gcgtggatat tggcaacctg cattatattg
    23821 ataccggcgc tgtctttggg ggcgaactga ctttcgtgca attgcaataa ttaaaaatca
    23881 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
    23941 ccatgacgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
    24001 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
    24061 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
    24121 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
    24181 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
    24241 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
    24301 ggtctgattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
    24361 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
    24421 agctgccggt tgtggtacga ccagtccgat cgcaccattt ttcagcctag ttgaggccgc
    24481 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
    24541 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
    24601 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
    24661 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcttgcggt gtcagttcaa
    24721 aagcgttctc gttgtcgggg gaggcaaggg caataaccgc ttcatgctgc cagcgataac
    24781 ctttgttgtg cgtttcggca agtttaatcc ccaggcgaga aaatcggact ttaccgatca
    24841 gggcttcaat gcctaccgga aacaaccaca cttctttgtc gcgctgccag agtcgcaggt
    24901 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtgcg gcttcgcgat
    24961 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggtgggt aaggcgggaa
    25021 tggcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
    25081 aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc accagggaag agatcgccaa
    25141 gtggtaaaaa ctctactgcg tcggggtaag tctctttaag ccacaggcaa acggattcgt
    25201 tttcttcccg gtttaaggta caggtcgagt aaaccagtgt accgccagag cgtaatgcat
    25261 gaaaggcact gtcgatcagt tcccgttggg tagctgcgat ttcctgattg ctttctggtg
    25321 accaattttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
    25381 ccagcaaaat ggcatcgaac atttctggca atgccgcacc aaacacgcgg ccatcaaaat
    25441 gtgtgagcgc aacattactg ataccacagc ggctgatatt ggcatgtaac acttttaccc
    25501 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
    25561 gcgtcgtttt ggagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
    25621 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
    25681 ttaaatgctc ggcggtacta cccaatggca atgcatcttc atcgtcgcgt tcaatccaga
    25741 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaatcgca
    25801 ggaaatcagc aacggaggtt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
    25861 gacaggcggc aagaaaatca tcaaaagaga gcgtcgaagg cattgcttcg cgcatttgcg
    25921 tcagaaaggc gtccgggaaa taaacggtgt gttgggccac gagcatatac cactggcaaa
    25981 aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga gcagtgggca
    26041 ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt cctgcaacag
    26101 gaagtgcttg ccttcctggg ctttcggtgc cagtggcgta cccggaggcg tggcgaaggc
    26161 gatgccgcca cggataaact ggttaaaggt gccggttttc actacgccgc ccgtcagacc
    26221 aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca ccaggtgttg
    26281 atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat
    26341 tccggtaacc gtaccgactt ccagaccacg gaacagcaca ggcgtaccga tgcctaacga
    26401 acaggcttcc ggcgcttcaa caataatgct taaaccatcc aggtaacgcg aatcagtaat
    26461 ggtggcctct tgtaattcaa agtcgcgacg aggattgcca cggcctggtt cgacgttgat
    26521 atacggctgg aggatagtat caagatgctc aacgccagct gccgaaattt gcggtgtgac
    26581 cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat agagcaccgc
    26641 ctttgcctgt acttcattgc gcgcggtaat cagatccagc gtctggattt gcccgatatc
    26701 aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga aagcatgaag
    26761 cgtaatctgc ccgccaaccg cacgggccgc tgtttcggaa gcatacagaa tacgcttatc
    26821 gcctttacgc tgactggcgc tggcaccgct gaggttatcg aagctaatgg ctccctttaa
    26881 tgctctggag agtggggatg cctgtacggt caggccacta ccattcagct gaactttcgc
    26941 cccgccttct gcccagaaca cgctattgtt ggtcagaagg ttgcgatact ccggcttaat
    27001 atgcagatcg atatcaaacg cgttagctcg cggacgcacg gtaataactt caccaacttc
    27061 aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa
    27121 actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt ccagattggc
    27181 atacagtgga tagctggctt tcatctcacc tttatcgccc ggcagaatac gtatcccgcc
    27241 attgatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc ccaccttcac
    27301 atcgacacgg ctgttgacga caaatttgct atcgcctttt accagttcgc gatgctgagg
    27361 ctcaatggcg acggtaaagg taacgccttt gctggtgagt ttacgatcaa taacctggcc
    27421 tacctgcacg ccgtgaagaa tgagcggctg accagcatca ataccgtaac tttccggtgc
    27481 tgtcagggtc agtgtcagaa catcaggttc atgcagcagt gctttttcgc ctggcacaac
    27541 aacgaactct ttgcgtggct cgccatcgcc aggcaccaac tcaaaggttt ttccggtcag
    27601 cagggcgctg agattggcat cgctaaggga taatttcggg ttgcgtaatt cgatgcgggt
    27661 attttcccgt aacagcgtaa cgacgctggg atcaacggtc atttctccgg tgactttacc
    27721 accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat acattaacgg
    27781 cgtcgagtcg gcggttaatc cggccccact cggcagttcc agttttatta ttacgccacg
    27841 ctggctgtgg gccagatctt catacagacc aaaggtatct tccgcctcgg caggtttcga
    27901 ctcttctggt gaatcgaagg caatcgcacc gttaaccagt gccgccagac tttccagttt
    27961 caccttcgcg ccactgatac tgacgttggc atcaacgcct gaaacgttcc agaaacggct
    28021 accttttttc accaggtcgg taaaacgccg ctcgatcagg acatcaatca ccacgccttg
    28081 tttattggga ttgatggcat agtcgtagac ttttcccacc gggatcttgc ggaaatagac
    28141 caatgaaccg ctgttcagcg aaccgagatc gggagcttgc aggtggatca tcagatcgcc
    28201 gttatccagc cgatacttcg gttgggtatc gagcgcgaca aagtgatcct gctctttacc
    28261 tttacctggc atcatgccga tatagttacc accgacgagg gcgtccagcc cggagacacc
    28321 tgccaacgat gcttttggcg tcaccagcca gaactgagtc tcttcgcgca gcgcatcttt
    28381 catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc tgaggctgat
    28441 atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc ccgggacaat
    28501 accatccgcc gacataaagt cgatggtgac ggtattaccc cggtcctgat aactgtccca
    28561 aatcagccaa ccggcaatca ttagcgcgat gaacggcagc agccagaaag gtgagatacg
    28621 gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac tcatgtgcat
    28681 cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt accgctgcgc
    28741 cgaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga ttaatcagcg
    28801 acatggttaa agatatgaca aacaggtcga gcatcgacca gcgaccaatc caggtcacca
    28861 tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga atgctcaaca
    28921 gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg ataaacacga
    28981 ttcctgcaac cgcaatgttg ctacttgcca gcgacataat tccggaaaga attgtatctt
    29041 cctgccgtcc tccgttcaga taaatgatag aaataggcaa caggttggca ggtaacaata
    29101 aaacgattga cgctaacagc gccgcccagc atttttgcag actatgacgg cgacgcaggc
    29161 gtagcggaat atgacaacgc gggcagcgac cacgctgatc aggatagccg gtaaaatggc
    29221 acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc ggataaaatc
    29281 gctcccacag ctcctcgaca ttaagatgtg acaacgtcac cgtcgttaaa atcaccaacg
    29341 ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt acctttatag
    29401 aagcaacgcc aatgccgacc aggtagatat cgagcattac ccactcttta agtcgctcaa
    29461 gcatcagcag taccgggcgt agattcattc ccagtcggtt accaaaccat aaataagcta
    29521 tggaggtcac cagaatgagc ggggcaccaa taacgcagaa aaagaccatc gaccccgtta
    29581 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat aacgttggcg tcaatacgaa
    29641 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcatc agcaacagca
    29701 tggtgaaggc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtca cgaatttttg
    29761 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
    29821 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
    29881 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
    29941 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
    30001 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt atataatgaa
    30061 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
    30121 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tatgagcgtc tcactgacgt
    30181 aaactgggca ggtttttatt tgcttgagga cgatacactg gtgctcggac catttcaggg
    30241 caaaattgcc tgtgtccgga tacccgtcgg gcgtggcgtg tgcggcactg cggttgcccg
    30301 caatcaagtg cagcgtatcg aggatgttca tgcgtttgac gggcatattg cctgtgatgc
    30361 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
    30421 cgacatcgat agtaccgtct tcggtcgctt tacagacgag gacgagcaag gcttacgtca
    30481 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
    30541 cgtcgcagga taatcaacgg ataacgtagc atttactgat agcgtcatta taatgacgcc
    30601 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
    30661 acctaagttg aatagcagta aagaagtaat cgcgtttctg gccgaacgtt ttccccactg
    30721 tttcagtgcg gaaggtgaag cgcgtccgct gaaaatcggt atttttcagg atttggtcga
    30781 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
    30841 cacttcgagc tggcgttacc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
    30901 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
    30961 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
    31021 tgccgcagct gctggtgaga aagaagacgc accgcgtcgc gaacgcaagc cacgtccgac
    31081 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
    31141 agcgccaaaa acagcaaaag cacctcgcga agaacagcac accccagttt ctgacatttc
    31201 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
    31261 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
    31321 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg aacatgtttt
    31381 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
    31441 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtga
    31501 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
    31561 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaacgtgc
    31621 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
    31681 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgtcgttttg
    31741 aacgttacca atacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
    31801 cttataacct tgaccgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
    31861 tgtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctggcagga aaaacggata
    31921 aagaaattcg tgaaaccctg actcgccgct acaaatttgc cattcgtcgt ctggcgcaaa
    31981 ccaacagcga agatgttttc tcgctggcaa tgacggcgtt tgcgcgtgaa atcgacccgc
    32041 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcgc
    32101 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
    32161 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
    32221 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
    32281 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
    32341 ggaccaagac ccgcactgta acattgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
    32401 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attcctggct
    32461 tctatgtggg tttgacagac gatgtcaaag tgcaactgca gaaactggaa aaacagaatg
    32521 tcagcagtgt gatcatcgat ctgcgtagca atggtggtgg ggcgctgacc gaagcggtat
    32581 cgctctcagg tctgtttatt cctgcgggtc ctattgttca ggtccgtgat aacaacggta
    32641 aagttcgtga agacagcgat accgacggac aggttttcta taaaggcccg ctggtggtac
    32701 tggttgaccg cttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
    32761 gtcgtgcact ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
    32821 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
    32881 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
    32941 cgccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
    33001 ataacgcgct gccgtgggat agcattgatg ccgcgactta tgtgaaatca ggcgatttaa
    33061 cggcctttga accggagcta ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
    33121 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
    33181 tttctctgaa ttacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtttgg
    33241 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaaa ctggatgatc
    33301 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
    33361 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgcacccgtc aagtaatatc
    33421 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
    33481 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
    33541 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
    33601 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
    33661 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    33721 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    33781 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    33841 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    33901 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    33961 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    34021 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    34081 ggtgaacacc ttcgttatct ttatttcccg tatcctggcg cagcttgccg cgggttttat
    34141 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    34201 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    34261 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    34321 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    34381 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgtttatga ctcacccgcc
    34441 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    34501 tactaaaaag cgcgtcctgc tcgacgcgct ttttttatgc cctggatctt ggctgagtga
    34561 tacgtaaccc actgatacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    34621 cgtgtgtacc attatctcca aactgattta gcatcagcgc caccagcgcc gcgccgctac
    34681 tctgacccag tagacgagcc gttcctaaca tgccactggc tccaccgcta cgttcgcgag
    34741 gcgcggaggt aataatggtg tggttattgg gtgactggaa caagccaaat ccagcgccac
    34801 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    34861 aaaaaagccc cgcagccatg atgaataatc ctaaagcacc cagcaatcct gcatgtacgc
    34921 gttcaatcaa atagcctgcc aacggagcca tcaccatcgt tgctaacggc cacggtgtca
    34981 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    35041 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    35101 acagcgggat acgcagtaaa tccaccggta gcagcggtac tggaagagaa agctggcggc
    35161 gaataaagaa aataccaacg acaaccatta caaccagttc cgcagcaatt aacgtcagcg
    35221 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    35281 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    35341 aacgcatcgc cagaagcagg gcgataatac ccaacggtac gttgattaaa aataaccatt
    35401 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    35461 caacaataaa cgagtttatg cccatccctc tacccagaaa acgttgtgga tagatcaggc
    35521 ggataagggc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    35581 caagggtgag tatttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    35641 cgaccagacc gcatttataa atacgtcgat agccaaacat atcacccaga aacgaaaacg
    35701 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    35761 gcgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    35821 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    35881 gcaggccgtc ggcctgaact tttggcatag gaattttaaa tctttggtga ataatcaaca
    35941 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    36001 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    36061 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    36121 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    36181 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    36241 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    36301 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    36361 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    36421 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    36481 tattcacgga ttgggcgtcg taatccactg tacagcaccg cgattggtaa ggtactgctg
    36541 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    36601 accgagcgga ccatcaccag tacagaagcg ttattaccgg ttctggacca ggtgcgcgag
    36661 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggttccg
    36721 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    36781 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    36841 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    36901 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg gcgttagatt
    36961 aaacgcagtt gatataagaa tattttactc atgagtgaga atctggttat ttattacact
    37021 taaccattat cgaccacgat attgcttttg cgtaacagcg ggcaatctgt tatccccaaa
    37081 aaaccacttt tagtgtgcaa gtattgtacc gtgctggtgc ctctggcagt cagataggta
    37141 cattgcaaac ctaattctgc ggcattctct ttgcttccaa tcaaaacgcc atatccgctg
    37201 agtaataatc ctatccatac cagtgctatc agcataactg tgcgaatgat gaatcgcatt
    37261 acaacctctt ctctttttta tgttcgctta atcgtagcgg caatatgcgc tgaagcaagc
    37321 gactcattcc gaaaaagcac gaatatcgac atagttagac gctgtttaac taacgcatgg
    37381 tagtttaatg acataaggta ggtgaaacgg agattggagt gaaaaagttt cgatgggtcg
    37441 ttttggttgt cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc aacatgatgt
    37501 gcgatcagga tgtacaattt ttcagcggaa tttgtgccat taaccagttt atcccgtggt
    37561 gatatcattt tttcagcact agattttctt cctctccagc agtggtagaa tagcgcctct
    37621 actcagaggt ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca ttgcgtcttt
    37681 ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg tggtttttta ttaatcgtgc
    37741 cagttctcgg actaacgaac agattgaact gcttgaggcg ttgctggatc agcaaaaacg
    37801 tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa ccagagaaag cagataaaaa
    37861 gactattgag agtcaaaaat cggttgaaga cgaagatatt attcgcctgg tcgccgaacg
    37921 ataatccttc ctcaatcttg agtaaccccg tcgttgaagg gttactcaat tcatcctgat
    37981 tatacgcact gtcatggtgc aactgcttca aattaaaact ctggtctgac ttcaaataat
    38041 tatcatatta tttatttgcg taacgggata tcagaaaaag atatttaccc cattaattta
    38101 ttaggatg
//
LOCUS       DAZFKY010000022        37834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFKY010000022 DAZFKY010000000
VERSION     DAZFKY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(95..790)
                     /gene="aqpZ"
                     /locus_tag="ACHKCL_001035"
     CDS             complement(95..790)
                     /gene="aqpZ"
                     /locus_tag="ACHKCL_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5762052.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1216..2874
                     /gene="ybjD"
                     /locus_tag="ACHKCL_001036"
     CDS             1216..2874
                     /gene="ybjD"
                     /locus_tag="ACHKCL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5762053.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2871..3827)
                     /gene="ybjX"
                     /locus_tag="ACHKCL_001037"
     CDS             complement(2871..3827)
                     /gene="ybjX"
                     /locus_tag="ACHKCL_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5762054.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3978..5093
                     /gene="macA"
                     /locus_tag="ACHKCL_001038"
     CDS             3978..5093
                     /gene="macA"
                     /locus_tag="ACHKCL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5762055.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5090..7036
                     /gene="macB"
                     /locus_tag="ACHKCL_001039"
     CDS             5090..7036
                     /gene="macB"
                     /locus_tag="ACHKCL_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5762056.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7109..7333)
                     /gene="cspD"
                     /locus_tag="ACHKCL_001040"
     CDS             complement(7109..7333)
                     /gene="cspD"
                     /locus_tag="ACHKCL_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5762057.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7483..7557
                     /gene="yljB"
                     /locus_tag="ACHKCL_001041"
     CDS             7483..7557
                     /gene="yljB"
                     /locus_tag="ACHKCL_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5762058.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7656..7976
                     /gene="clpS"
                     /locus_tag="ACHKCL_001042"
     CDS             7656..7976
                     /gene="clpS"
                     /locus_tag="ACHKCL_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5762059.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8007..10283
                     /gene="clpA"
                     /locus_tag="ACHKCL_001043"
     CDS             8007..10283
                     /gene="clpA"
                     /locus_tag="ACHKCL_001043"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5762060.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10629..10716)
                     /locus_tag="ACHKCL_001044"
     tRNA            complement(10629..10716)
                     /locus_tag="ACHKCL_001044"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10680..10682),aa:Ser,seq:gga)
     gene            complement(10970..11188)
                     /gene="infA"
                     /locus_tag="ACHKCL_001045"
     CDS             complement(10970..11188)
                     /gene="infA"
                     /locus_tag="ACHKCL_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5762061.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11473..12177)
                     /gene="aat"
                     /locus_tag="ACHKCL_001046"
     CDS             complement(11473..12177)
                     /gene="aat"
                     /locus_tag="ACHKCL_001046"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5762062.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12219..13940)
                     /gene="cydC"
                     /locus_tag="ACHKCL_001047"
     CDS             complement(12219..13940)
                     /gene="cydC"
                     /locus_tag="ACHKCL_001047"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5762063.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     PLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13941..15707)
                     /gene="cydD"
                     /locus_tag="ACHKCL_001048"
     CDS             complement(13941..15707)
                     /gene="cydD"
                     /locus_tag="ACHKCL_001048"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5762064.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15830..16795)
                     /gene="trxB"
                     /locus_tag="ACHKCL_001049"
     CDS             complement(15830..16795)
                     /gene="trxB"
                     /locus_tag="ACHKCL_001049"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5762065.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17340..17834
                     /gene="lrp"
                     /locus_tag="ACHKCL_001050"
     CDS             17340..17834
                     /gene="lrp"
                     /locus_tag="ACHKCL_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5762066.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17969..21997
                     /gene="ftsK"
                     /locus_tag="ACHKCL_001051"
     CDS             17969..21997
                     /gene="ftsK"
                     /locus_tag="ACHKCL_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5762067.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            22152..22763
                     /gene="lolA"
                     /locus_tag="ACHKCL_001052"
     CDS             22152..22763
                     /gene="lolA"
                     /locus_tag="ACHKCL_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5762068.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22774..24117
                     /gene="rarA"
                     /locus_tag="ACHKCL_001053"
                     /gene_synonym="mgsA"
     CDS             22774..24117
                     /gene="rarA"
                     /locus_tag="ACHKCL_001053"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5762069.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24208..25500
                     /gene="serS"
                     /locus_tag="ACHKCL_001054"
     CDS             24208..25500
                     /gene="serS"
                     /locus_tag="ACHKCL_001054"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5762070.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25739..28183
                     /gene="dmsA"
                     /locus_tag="ACHKCL_001055"
     CDS             25739..28183
                     /gene="dmsA"
                     /locus_tag="ACHKCL_001055"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5762071.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28194..28811
                     /gene="dmsB"
                     /locus_tag="ACHKCL_001056"
     CDS             28194..28811
                     /gene="dmsB"
                     /locus_tag="ACHKCL_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5762072.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28813..29676
                     /gene="dmsC"
                     /locus_tag="ACHKCL_001057"
     CDS             28813..29676
                     /gene="dmsC"
                     /locus_tag="ACHKCL_001057"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5762073.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29712..30338)
                     /gene="ycaC"
                     /locus_tag="ACHKCL_001058"
     CDS             complement(29712..30338)
                     /gene="ycaC"
                     /locus_tag="ACHKCL_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5762074.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30653..31801
                     /gene="ycaD"
                     /locus_tag="ACHKCL_001059"
     CDS             30653..31801
                     /gene="ycaD"
                     /locus_tag="ACHKCL_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762075.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32011..33441
                     /gene="ycaM"
                     /locus_tag="ACHKCL_001060"
     CDS             32011..33441
                     /gene="ycaM"
                     /locus_tag="ACHKCL_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5762076.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33442..33552)
                     /locus_tag="ACHKCL_001061"
                     /pseudo
     CDS             complement(33442..33552)
                     /locus_tag="ACHKCL_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33651..34391)
                     /gene="pflA"
                     /locus_tag="ACHKCL_001062"
     CDS             complement(33651..34391)
                     /gene="pflA"
                     /locus_tag="ACHKCL_001062"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5762077.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34583..36865)
                     /gene="pflB"
                     /locus_tag="ACHKCL_001063"
     CDS             complement(34583..36865)
                     /gene="pflB"
                     /locus_tag="ACHKCL_001063"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5762078.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36920..37777)
                     /gene="focA"
                     /locus_tag="ACHKCL_001064"
     CDS             complement(36920..37777)
                     /gene="focA"
                     /locus_tag="ACHKCL_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5762079.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9355 a         9273 c        10045 g         9161 t
ORIGIN      
        1 ggggaacgct tcagcgattt tgacgccacc aatacccgtg cggtttgtaa tgtcgaagat
       61 aaaacaacca gggcagataa aatgccaatg ctaattaatt acgcttttcc agcagagtcc
      121 ggtaaatcag accaccgata atgccaccga caattggcac cacccagaag aaccacagtt
      181 gttctaatgc ccagccgccc tggaagatgg caaccgcggt gctgcgcgcc gggttaacag
      241 aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga ccaatagcga
      301 tcggcgcaaa acctgccgga gcgaatttgt cggttgcacc gtggatcact aacaggaagc
      361 ccgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg cctggtgaat
      421 gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac
      481 tggcaattaa atacagcagc gctgccgcaa caataccgcc gacaacctgg gcaattacgt
      541 agccaacgac ttcttttgcc ggaaaacgtc cgccagccca taaaccaata gtgaccgccg
      601 ggttaaaatg accaccagaa atatgaccaa cagcaaaggc catcgtcaga acggtcagac
      661 cgaacgccaa cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct gcggccagta
      721 cagcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca gctgctaatt
      781 ttctgaacat atccacctca attaaaaatt gaccctgtga aaaatatggt cgttttatag
      841 ggtcgtcgta aaaaagtgac gacggaaata atgcgcggct attttaaaaa cgaaggcgag
      901 tcattcacca gataaataaa tccagtaaat ttgatttagg gcaacagcgg gttgccccat
      961 atagtcattt gtctgattga cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa
     1021 taaaaaataa tttatcatgc taattatttg attttgttgt ttttgcagac ttatcagcaa
     1081 gagggagtat aacgcgatta ttcgctcatt tttcagacat ttgccatgct taaatgtgat
     1141 gtcatcacgt attagcaagg cctttcccgt tatactgcca gcgtaaagga taagtcacat
     1201 atttctggag gggatatgat tcttgagcgc gttgaaattg tgggttttcg cggtatcaac
     1261 cgtttgtcgt tgatgctgga acaaaacaac gtcctgattg gggagaacgc gtggggtaaa
     1321 tccagcttgc tggacgcctt aaccctgctg ctatcgccag aatcagatct ctaccatttt
     1381 gagcgcgacg atttctggtt cccgccggga gatatcaacg ggcgagaaca tcatctgcat
     1441 attattttga ccttccgcga atcgctgcca ggtcgacatc gggttcgccg ttatcggccg
     1501 ctggaagcgt gctggacgcc atgcaccgat ggctatcacc gtatttttta tcgtctggaa
     1561 ggggagagtg cggaagacgg cagcgtgatg acactgcgca gttttctcga taaagacgga
     1621 catccgattg atgttgagga tattaacgat caggcacacc atctggtgcg tttaatgccg
     1681 gtgctgcgct tgcgtgatgc ccgttttatg cgccgtattc gtaacggcac ggtgccaaat
     1741 gtccccaatg tggaagtcac cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca
     1801 catccgcaaa atctctctga tggacagatt cgtcagggac tttccgcaat ggtgcagttg
     1861 cttgagcatt atttctctga gcagggggct ggacaggcgc gatatcgttt aatgcggcgg
     1921 cgggccagca atgagcaacg aagctggcgc tatctggaca tcatcaaccg gatgattgac
     1981 cgacctggag ggcgctcgta tcgggttatt ttgctcggcc tgtttgctac tttattgcag
     2041 gcaaaaggca cattgcgact ggataaagac gcccgtccat tgttgctgat cgaagatcca
     2101 gaaacgcgtt tacaccccat tatgctttca gttgcctggc atctgttgaa tcttttgcct
     2161 ttacaacgta ttgctaccac caactctggc gagctgcttt cgttaactcc agtggaacat
     2221 gtttgccgcc tggtgcgtga atcttctcgt gttgccgcct ggcgtctggg gccaagtggc
     2281 ttgagcaccg aagacagccg ccgcatctct tttcacattc gttttaatcg tccgtcatcg
     2341 ctgtttgctc gctgctggtt gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa
     2401 ctggcgcgtc agtgcggaca tcattttgat gccgaaggga tcaaggtcat tgagtttgcc
     2461 cagtccgggc taaagccact ggttaaattt gcccgccgaa tggggattga atggcatgta
     2521 ctggtcgatg gcgatgaagc agggaagaaa tatgccgcta cggtacgcag cctgttgaat
     2581 aatgatcggg aagccgaacg agaacattta acggcgttac cggcgctgga tatggaacat
     2641 tttatgtatc gccagggatt ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat
     2701 gtcccgatga atctgcgcaa aattatctcg aaagcgatcc atcgctcttc caaacccgat
     2761 cttgccattg aagtggcaat ggaggccgga cgtcgtggtg tggactccgt accgacgctg
     2821 ctgaaaaaaa tgttctcacg cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga
     2881 aacatcgtgg ccatttgtgg ctgaatagcg tcgagcatct catagcgccg acggtattca
     2941 gcccgttttt tactggcgat ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg
     3001 taatggcgtt cagcatcaca tacgccgcca accgattccc agaaagcgtt gtaatcggca
     3061 tggatcttgc cttctttatc gcgataacgc aggctgcggt aaatatgcgt ttcattgctg
     3121 acggcaataa tctgctctac ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg
     3181 aggcgtttgg gaaatagccc gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga
     3241 atttcccatt ttgcgccttg cagtccacca ataaacatcg ttcttttccc ctgatattca
     3301 cacagggtaa acgtgatttc tgccagagga ataccttcgc tgttgcggaa caggattgtg
     3361 ctgtcacctt ctttatccat tgagatcatc atggtcagct caagcgtgaa ctgctcgccg
     3421 tttttgcctt ccagcttcgc cagttgcagc ccgggggtat tcaaatataa gctgaattct
     3481 tccgccgaca tacatccacg gagtaacgca taatggtaac gtaacgcctc cagcaattgc
     3541 ttacggctaa gattcgccgc aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg
     3601 cgcgttaaca atacattgag attaggccag tgggaaagtt cgttcatcca ctcaacgctt
     3661 aaacgcggca taatcaacga gcgcagcaaa aatttctggc gaaaactacg gcgatgccag
     3721 aatttacccg gccgacactg tccacgtgcc agactaagaa aaagtgacag gctgctgaga
     3781 gattcagatg acgtaaaggt ccgttcggtt agctgcgaca tattcatgaa atcaatggtt
     3841 gtacatgacg tcgatttcac cattgcgtat cttaaccaaa catcaatagt gtgattacta
     3901 acgtaaattt tagggttttg ttgatatttc gttgaagtta atgacccgga ttggcatatg
     3961 gagtattcag aatatttatg aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc
     4021 tggtgatagt catcgccgga ctgattacgt tatggagaat tcttaacgca cccgtgccga
     4081 cttatcagac actgattgtg cgccccggtg atttacagca aagcgtgctg gcgaccggaa
     4141 agctggacgc gctgcgtaag gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaacgc
     4201 tgtcggtggc gattggcgat aaagtaaaaa aagaccagct tttaggggtt attgatcctg
     4261 aacaggctga aaaccagatc aaggaggtcg aagcaacgct gatggagcta cgcgcgcagc
     4321 ggcagcaggc ggaagcggag ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc
     4381 tggcacaaac gcaggcggtt tcactgcagg atctcgacac cgccgcgacg gagatggctg
     4441 tgaaacaggc gcaaattggc accattgacg cgcaaatcaa gcgcaatcag gcttctctcg
     4501 atacggctaa aaccaatctc gattacactc gcatcgttgc cccgatggcc ggggaagtca
     4561 cgcaaatcac cactctgcaa ggccagacgg tgattgccgc acaacaagca ccgaacattc
     4621 tgacgctggc agatatgagc accatgctgg taaaagcgca ggtttctgaa gcggatgtaa
     4681 tccacctgaa gccggggcaa aaagcctggt ttacggtact tggcgatcca ctgacgcgct
     4741 acgaggggca aatcaaggat gtactaccga cgccggaaaa ggttaacgac gctattttct
     4801 attacgcccg ttttgaagtc cccaacccca atggtttgct gcggctggat atgactgcgc
     4861 aagtgcatat tcagctcacc gatgtgaaaa atgtgctgac gatccctctg tcggcgttag
     4921 gcgatccggt tggcgataat cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg
     4981 agcgtgaagt gacgattggc gcacgtaacg ataccgatgt tgagattgtc aaagggctgg
     5041 aagcgggcga tgaagtggtg attggtgagg ccaaaccagg agctgcacaa tgacgccttt
     5101 gctcgaatta aaggatattc gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct
     5161 gaagggcatc accctcgata tttatgccgg tgagatggta gcgattgttg gcgcttcggg
     5221 ttccggtaaa tcgaccctga tgaatattct cggctgtctg gataaggcca ccagcggcac
     5281 ctatcgcgtc gccggtcagg atgttgccac gctggacgcc gatgcgctgg cgcaactgcg
     5341 ccgcgagcat ttcggcttta ttttccagcg ttaccatttg ctttcgcatt taaccgccga
     5401 gcagaacgtt gaagtaccag ccgtctatgc tggtcttgag cggaaacagc ggctgcttcg
     5461 cgcccaggag ttgctgcaac gactggggct ggaagaccgt acagagtatt atccggcaca
     5521 gctttcgggt ggtcagcaac agcgcgttag catcgcgcgg gcattgatga acggcggtca
     5581 ggtaattctt gccgatgaac cgaccggcgc actggacagc cattcaggcg aagaggtgat
     5641 ggcgatcctg catcagctgc gcgatcgcgg gcatacggtg attatcgtca ctcacgatcc
     5701 gcaggtcgct gctcaggccg agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa
     5761 tcctcccgcc attgaaaaag tgaatgttgc tggcgggacg gaacctgttg tcaacacggc
     5821 gtctggctgg cggcagtttg tcagcggttt taatgaggcg ctgacgatgg catggcgggc
     5881 gctggcagcg aataaaatgc gtactttact gaccatgctg gggattataa tcggtattgc
     5941 gtcggtggtt tccattgtcg tggtgggcga cgccgccaaa caaatggtgc tggcggatat
     6001 tcgttctatt ggtacgaata ctattgatgt ctatcccggg aaagattttg gcgatgacga
     6061 tccgcaatat caacaggcgc tgaagtacga tgacttaatc gccatccaaa aacaaccgtg
     6121 ggtcgcttca gccacacctg ccgtctcgca aaacctgcgc ctgcgttata acaatgttga
     6181 tgttgctgcc agtgccaatg gcgtgagcgg cgattatttt aatgtctatg gcatgacctt
     6241 cagtgaagga aacaccttta accaggaaca gctgaacggt cgtgcgcagg tcgtggttct
     6301 cgacagtaat actcgccgcc agcttttccc ccataaagca gatgtggttg gcgaggtgat
     6361 tctggtcggc aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt
     6421 tggtagcagt aaagtgctgc gtgtctggct accttacagc acgatgtccg ggcgagttat
     6481 gggccagtcg tggctgaact ccattactgt cagggtgaaa gaaggatttg acagcgccga
     6541 ggcggaacag caactcacgc gtttactttc actgcgccac ggtaagaagg atttctttac
     6601 ctggaacatg gacggcgtct tgaaaaccgt tgaaaagacc acacgtactt tacaactgtt
     6661 tatgacgctg gtggcggtga tttcgctggt agtgggcggt attggtgtaa tgaatattat
     6721 gctggtgtca gtgaccgagc ggacgcggga aattggcatt cgcatggctg taggtgcgcg
     6781 agcaagcgat gttttgcaac agttcctgat cgaagccgta ctggtttgcc tggtcggtgg
     6841 cgcgttggga ataacactgt cactgttaat tgctttcacc ttgcagcttt tcttacccgg
     6901 ctgggagatt ggtttttcac cgttggcact gctgctggcg tttctctgct cgacggtcac
     6961 cgggatttta tttggctggt tacccgcacg aaatgcggca cgactggatc cagtagatgc
     7021 tctggcacga gagtaatttt tgagataaaa atgccagccg atcgggctgg cattttgcct
     7081 ttaggatgta cacaatgaga cagaagagct atgcgactgc cgcttctact tcgacgggca
     7141 caataacact ggcgtgattg ccttttggcc cctggtggac atcaaactga acggattgtc
     7201 cagcttttag cgttctgtaa ccatccatct gaatggtgga ataatgagcg aaaatatctt
     7261 cgccgccgcc ttcagggcag atgaaaccaa accctttggc attgttgaac cacttaacag
     7321 tacccttttc catgcttcga catccttcgc aaatcttata caagtaagat ggaataaacc
     7381 ggggtcagag agggggctgt tcaaaacctc gccaactcta gaaatacaat ttagagaatt
     7441 agggcgagcc gtcaagcatt tgacagggga cagggggcag gtatgaatca aaaatttgaa
     7501 gcagttaacg ctattgacag gaatgtgaca gatgtcgctg atgccaacga tagatgatag
     7561 ttatctatca tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggcgtt
     7621 ctgccgataa ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg
     7681 actttgatca actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata
     7741 aagtgatatt agtcaatgat gattacactc cgatggagtt tgttattgac gtgttacaaa
     7801 aattcttttc ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg
     7861 ggaaggccat ttgcggagtc tttaccgccg aggttgcaga aaccaaagtg gcgatggtga
     7921 acaagtacgc gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc
     7981 aggcataaaa attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata
     8041 tggctttcgc cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac
     8101 tggcgctgct cagtaaccca tctgcccggg aggcgctgga agcgtgttct gtggatttgg
     8161 ttgcgctccg tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca
     8221 gtgaagagga gcgcgacaca cagccgacgc tgagttttca gcgtgtactg caacgtgcgg
     8281 tcttccatgt ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta
     8341 tctttagcga acaggagtcg caggcggcat atctgttgcg taaacatgaa gtcagccgtc
     8401 tcgatgtggt gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg
     8461 atcctggcag ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atggagaatt
     8521 tcacgacgaa cctgaatcag cttgcgcgcg tgggcggaat cgacccactg attggtcgtg
     8581 agaaggagct ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc
     8641 tagtggggga atctggtgtc ggtaaaaccg cgattgcaga aggtcttgcc tggcgaattg
     8701 ttcagggcga tgtgccggaa gtgatggctg actgtacgat ttactctctc gatatcggtt
     8761 ctctgttagc gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa gcgttgctca
     8821 agcagctgga gcaggacact aacagcatcc tgtttattga tgagatccac accattatcg
     8881 gtgcgggtgc agcgtctggt ggtcaggttg atgcggctaa cctaatcaaa ccgttgctct
     8941 ccagcggtaa aattcgcgta attggttcga caacctatca ggagttcagc aacattttcg
     9001 agaaagaccg tgctctggcg cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg
     9061 aagaaactgt tcaaatcatc aatggcctga aaccgaagta tgaagcacac cacgacgtgc
     9121 gttataccgc aaaagcggtg cgtgcggcgg tagagctggc ggtgaaatac attaacgatc
     9181 gtcatctgcc ggataaagcc attgatgtta tcgacgaagc gggcgctcgc gcacgtctga
     9241 tgccggtaag caaacgcaag aaaaccgtta atgtggcgga tattgagtcc gtggtggccc
     9301 gtattgcacg cattccagag aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc
     9361 tcggcgatcg cctgaaaatg ctggtcttcg gtcaggataa agccattgag gcgctgactg
     9421 aagccattaa gatggcgcgt gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc
     9481 tgtttgccgg ccctaccggg gtcgggaaaa cagaggtgac ggtacagctt tcgaaagctt
     9541 tgggcattga gcttctgcgc tttgatatgt ccgagtatat ggaacgccat accgtcagcc
     9601 gtcttattgg tgcgcctccg ggatacgttg gttttgatca gggcggtttg ctgactgatg
     9661 cggtcatcaa gcatccacat gcggtgctgc tgctggacga aatcgagaaa gcgcacccgg
     9721 acgtgttcaa tattctgttg caggtgatgg acaacggtac gctgaccgat aacaacggac
     9781 gcaaagcgga cttccgtaac gtggtactgg tgatgaccac taacgccggg gtacgggaaa
     9841 ctgagcgcaa atccattggt cttatccacc aggataacag caccgatgcg atggaagaga
     9901 tcaagaagat ctttacaccg gaattccgta accgtctcga caacattatc tggttcgatc
     9961 atctgtcaac tgacgtgatc catcaggtgg tggataaatt catcgtcgag ttgcaggttc
    10021 agctggatca gaaaggtgtt tctctggaag tgagccagga agcgcgtaac tggctggccg
    10081 agaaaggtta cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc caggacaacc
    10141 tgaaaaaacc gctcgccaac gaactgctgt ttggttcgct ggtggacggc ggtcaggtca
    10201 ccgtcgcgct ggataaagag aaaaatgagc tgacttacgg attccagagt gcacaaaagc
    10261 acaaggcgga agcagcgcat taatctgatt gtcaggtagg ttggtgaagt ccgtaatctc
    10321 gaaagaggtt acggactttt tatttatggg gtggaacggt gaaaacccta tttttggagg
    10381 tgaaggtaat ttgttgataa ttagtgctgc tggaaaaggt aaggataaaa aagggtgaca
    10441 gcaggaaaat gagatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt
    10501 cactgccgta caggcagctt agaaattcac tggtaacata ctccacccgc ccaccatgtt
    10561 cactgccgta caggcagata aaatgcgaaa aaaaagcccg tactttcgta cgagctcttc
    10621 tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt
    10681 ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgctg ccaaacctca
    10741 tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa
    10801 tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac atcgagacaa
    10861 gttatggact tatacttcca aagtacttca tgcatatcac aaaataaaaa ggccggttaa
    10921 accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac
    10981 ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc
    11041 ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt
    11101 gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt
    11161 gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg
    11221 aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa
    11281 caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg
    11341 tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc
    11401 agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg
    11461 tgccgaaaac attcattctt gtggtgaaaa caagcatcgt ggtacccaga aattattcgg
    11521 caatcgtccg aggcgcattt gattgagata attaaggtaa ttccggcggg gaatttcgca
    11581 ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg
    11641 accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct
    11701 gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag
    11761 ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa ggcgatggta
    11821 ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg cacagccttc
    11881 aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg
    11941 cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga
    12001 ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct ggtaagccat
    12061 taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa
    12121 tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca ggcgcatgac
    12181 cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg
    12241 taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat aatttgcccg
    12301 ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt
    12361 aacaccgttt tctcacgcat catttctgca agcaattcaa ggatctggct ttcggttgtg
    12421 gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgca
    12481 cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac
    12541 caactgttga gacctgcatc ctcgagctgc ttctccagac caacgcgacg taagatcacc
    12601 gccagagcct catcactact gccaggcgag gcgagtaaca gattatcacg cagcgtggcg
    12661 ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc
    12721 aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg
    12781 gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat cgctatatgt
    12841 tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa
    12901 gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca
    12961 gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc agaggcaatg
    13021 acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac
    13081 gcgcaaaaga caaacagggc aattaacgcg ccgggttgag cattgccgcc aacgccgcca
    13141 gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc
    13201 gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc
    13261 tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc
    13321 agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg
    13381 ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataatgccg
    13441 cccagcgtaa aggcgagggg ggaatcaagg aaacttaacc cgattgtcac caccataatc
    13501 accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc gagcgtatca
    13561 acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag tccggcaggg
    13621 gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc
    13681 gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgctgcacca
    13741 cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc
    13801 gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtc
    13861 acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata cagtgccaga
    13921 tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat
    13981 gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc
    14041 ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt
    14101 gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatca
    14161 aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc
    14221 accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggcgta
    14281 tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct
    14341 tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg taatgttgct
    14401 gccggtaatt gtgggttttg accaacccag gagagatgtt tacgccatga ttctggtgat
    14461 aaatcgcgta attctatccc gttgattcgt aatgatccct gatatgagag aaaaccagaa
    14521 agcgcgttca gcagtgagct tttacctgaa ccgctgcgac caaccaacac tgcacgttgg
    14581 cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata
    14641 aacagatcct cggcctcaat ggtcagcgga tcggttgatg ctaattccgt ctcaccgcgc
    14701 tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc
    14761 tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca
    14821 aggatcaggg ccagaaaacc cgcagccagc gtcacgccag tatcgtagtg accaaaatcc
    14881 agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta
    14941 aaaaattcga gaatgccgga ggataaaaac gccagccgta gcacttccat tgtccgttgg
    15001 cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata
    15061 cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg
    15121 agaaagttac gtcggttagc atcggcagcc cccatcccaa ccagcgccat aaacaacgga
    15181 attaacggtg cagtgcccag cagaatgagc gccgcagccc agttagaggg gaagatcgcc
    15241 accacaatca gcaacggcac cgacactgcc agcgccattt gcggcaggta gcgtgcatag
    15301 taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc cgcaggttta
    15361 ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg gcggatggca
    15421 aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg
    15481 cgcagtacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata
    15541 ttctccataa tcatatgttg cagaattcgc gccatgaacc atgcctgggc aatgatcaat
    15601 atgccgctca caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt
    15661 tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc acgcttagca
    15721 ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc
    15781 catagtcgca tggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt
    15841 cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcct gtaccagcag
    15901 atgtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa
    15961 tgctggtctg ggtggcatta ccatgaatac ccgactgcac tttgatgtag ccgttttcca
    16021 gttccagctg cccttcgaaa atggcagtat tcgggctgtg accgatagca acaaacagac
    16081 cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag
    16141 tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt
    16201 tgccgttctc cactttatcc atcagacgct taatgaggat tttttccgcg cggaaaccgt
    16261 cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa
    16321 ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc
    16381 aggttgcaca agcagaaact ccacggcctt taaaggcttc ttcagagggc aggccgagat
    16441 agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc
    16501 cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct
    16561 cagtttcaaa cttggtggca tgttcgtgca tgcgctccat taataacgga ccggtcagat
    16621 cgtttggatc gccaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt
    16681 ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcatagaca gcagcggtgt
    16741 atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat
    16801 ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac gtaaaaaaag
    16861 agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc
    16921 tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga
    16981 ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg
    17041 acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta ttcttatatg
    17101 cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac gtatggcgtg
    17161 gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct caccgtagac
    17221 ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg tagcgactct
    17281 gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag acaataataa
    17341 tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt aacattctta
    17401 atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt gtgggacttt
    17461 ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt attcagggct
    17521 atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc gttgagatta
    17581 ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta caaaaacttg
    17641 aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg aaaacacgcg
    17701 tgccggatat gtcagcctac cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg
    17761 tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat cgtctggtta
    17821 ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac actcctgtta
    17881 atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag catcgggcag
    17941 gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag acaaagaagt
    18001 cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga tccttattgt
    18061 cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg accccagctg
    18121 gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg gtgcgtggtt
    18181 ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg tcattattgt
    18241 cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaaaacattg attattttgc
    18301 cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg gtctggcggc
    18361 aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca gcttactaag
    18421 cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc tctgcgtttg
    18481 ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg aaaaactcgg
    18541 cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg
    18601 ggtcgatgaa gatgagtatg aagacgacga agagtatgaa gatgaaaacc acggcaaaca
    18661 gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc
    18721 ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct ccggtaagcg
    18781 gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg acccggacga
    18841 cgtcctattt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg atccattatt
    18901 aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca cggcgacaca
    18961 aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct ctgttgatgt
    19021 tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa cgggagagcc
    19081 ggttattgct cctgcaccgg aaggttaccc acagcagtca caatatgcgc agcctgcagt
    19141 gcaatataat gagccgctgc aacaaccagt acagccgcag cagccgtatt atgcacctgc
    19201 agctgaacaa cctgcgcaac agccgtatta tgcccctgcg ccagaacaac cggtggcagg
    19261 taacgcctgg caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac
    19321 tgagcaaact tatcagcagc cagccgctca ggagccgttg taccaacagc cgcaacccgt
    19381 tgaacagcag cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc
    19441 gccgctttac tactttgaag aagttgaaga gaagcgagcc cgtgaacgtg aacaacttgc
    19501 ggcctggtat caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct
    19561 gaaagcacct tctgttgcag cagtacctcc agtagaagcc gctgccgctg tttctccgct
    19621 ggcatctggc gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc
    19681 agtcttcagt ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc
    19741 gcagttgcca cgaccgaaac gtatccgcgt gccaactcgt cgtgaattgg cgtcttacgg
    19801 tattaagctg ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca
    19861 gtacgattct ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact
    19921 ggcacgtcag ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt
    19981 gcccgtaaat gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc
    20041 gcaaactcaa caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct
    20101 ggatgatttt gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgct
    20161 gtttacgcca attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca
    20221 gtatcagcag ccacaacagc cggttgcgcc acagccgcag tatcagcagc cacaacagca
    20281 ggttgcgccg cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata
    20341 tcagcagccg caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt
    20401 tgcaccgcag ccgcagtatc agcagccaca acagccagtt gcgccacagc cgcaggatac
    20461 tctgcttcat ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac
    20521 gccgctgcct tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac
    20581 ctttgcgctt gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa
    20641 agccgatgtc gtcaattact ctccggggcc ggttatcact cgctttgaac ttaacctggc
    20701 accgggcgta aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc
    20761 gacggtggcg gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt
    20821 accgaataaa aaacgacaaa ccgtttatct gcgcgaagta ttggataacg ccaaattccg
    20881 cgataatcca tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt
    20941 tgccgatctg gcgaaaatgc cgcacttgtt agttgcgggg actaccggtt ccggtaaatc
    21001 tgtcggtgtg aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg
    21061 tttcatcatg atcgacccga aaatgctgga gctttcggtg tatgaaggca ttccgcacct
    21121 gttaacggaa gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa
    21181 cgagatggag cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta
    21241 taacgaaaaa attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa
    21301 gccgggtgac agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt
    21361 gttggtggac gaatttgccg acctgatgat gacagtaggt aaaaaagtgg aagagctgat
    21421 agcacgtctg gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg
    21481 tccatcggtt gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt
    21541 taccgtatcc agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact
    21601 gctgggtatg ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca
    21661 tggtgctttt gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg
    21721 tcgcccacag tatgttgatg gcatcacctc cgacagcgaa agcgaaggtg gtgcgggtgg
    21781 tttcgatggc gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga
    21841 aaaacgcaaa gcgtcaattt ctggcgtaca acgtcagttc cgcattggtt ataaccgtgc
    21901 agcgcgtatt atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg
    21961 taatcgtgaa gtgctgtccc caccgccgtt tgactaatta atgcattgcc ggataaggcg
    22021 cggtagcgtc gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa
    22081 gctgattatt agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt
    22141 gaggaataat gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa
    22201 gcagcgtttg ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct
    22261 tccacgccag cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc
    22321 agggcgatct gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg
    22381 aaagcattct ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag
    22441 ctacggcaac ctggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca
    22501 accagtccag cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga
    22561 cgccgaaagc cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca
    22621 caatccatca gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat
    22681 cccagcaaaa tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca
    22741 cggtagatga tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg
    22801 gataatactt ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc
    22861 ggtcagcaac atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat
    22921 ttacattcta tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg
    22981 attgcccgct atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggtgtgaaa
    23041 gagattcgcg aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt
    23101 ctttttgttg acgaagttca ccgtttcaac aaaagccagc aggatgcatt tctgccacat
    23161 attgaagacg gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt
    23221 aattcggcac tgctttcccg tgcccgtgtt tatctgttga aatccctgag tacagaggat
    23281 attgagcaag tactaactca ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat
    23341 attgttctgc cagatgaaac acgacgtgcc attgcagaac tggtgaatgg cgacgcgcgc
    23401 cgggcgttaa atacgctgga aatgatggcg gatatggccg aagtcgatga tagcggtaag
    23461 cgggtcctga agcctgaatt actgaccgaa atcgccggtg aacgtagcgc ccgctttgat
    23521 aacaaaggcg atcgctttta cgatctgatt tccgcactgc ataagtcggt acgtggtagc
    23581 gcacccgatg cggcgctgta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta
    23641 tatgtcgcgc gtcgctgtct ggcgattgcg tctgaagacg tcggtaatgc cgatccacgg
    23701 gcgatgcagg tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt
    23761 gaacgcgcca ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc
    23821 tacactgcgt ttaaagccgc gctggccgat gctcgcgaac gcccggatta tgacgtgccg
    23881 gttcatttgc gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat
    23941 cgttacgctc atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa
    24001 atagcacaaa cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa
    24061 aagctcgcct ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt
    24121 tatcgttgcg gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc
    24181 atttaattcg ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca
    24241 gacgcagtcg ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc
    24301 gctcttgaag agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt
    24361 aactcccgat cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta
    24421 cgtctggaag tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct
    24481 ttacaggctg aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg
    24541 ccggtaggta aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag
    24601 tttgactttg aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt
    24661 gcagctgcag ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc
    24721 atgcaccgcg cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt
    24781 gagaactatg ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg
    24841 aaatttgctg gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac
    24901 tatgcgctga tcccaactgc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc
    24961 gatgaagatg atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc
    25021 ggttcatatg gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa
    25081 atggtgcaga tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat
    25141 gcagaaaaag tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc
    25201 gacatgggct ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac
    25261 acctaccgtg agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag
    25321 gcacgttgcc gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct
    25381 ggtctggctg ttggtcgtac gctggttgca gtaatggaaa actatcagca agctgatggt
    25441 cgtattgaag taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa
    25501 tacccaattt ttctgaatct aaaaagcgcc tacgggcgct ttttttgtct ccctttgata
    25561 ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat
    25621 ctctttgatt ttcaaattat tcgatgtata caagcctata tagcgatctg ctatagaaat
    25681 aattacacaa tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat
    25741 gaaaacgaaa atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa
    25801 aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg
    25861 gattgcgcac gctgtcgata gcgccattcc aacaaaatca ggcgaaaagg ttatctggag
    25921 cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga
    25981 aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt
    26041 tcgcgcctgc ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa
    26101 atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga
    26161 agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga
    26221 atccatctat ctgaactatg gcaccggtac gctgggtggc accatgaccc gctcctggcc
    26281 gccgggaaat accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta
    26341 cggcgactac tcctccgcgc aaattgcgga aggtctgaac tatacctacg gcggctgggc
    26401 agatggcaac agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa
    26461 ccctggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca
    26521 gaaatctaat gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg
    26581 cgaagatgag tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc
    26641 gtacgtcatg atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg
    26701 ctacgatgag aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat
    26761 tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc aaatcaccgg
    26821 tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt
    26881 tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat
    26941 ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg
    27001 tgaaggttca tacagtttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac
    27061 cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct
    27121 acgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta
    27181 tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg aaatccttca
    27241 ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa
    27301 atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcactgga
    27361 tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt
    27421 tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca
    27481 acagtttact gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg
    27541 ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa
    27601 gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa
    27661 tccactgacc acgccatcgg gtaaaattga gatttattcg caggcgctgg ctgacattgc
    27721 cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg
    27781 ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta caggtttcca
    27841 ctataaatct cgcgttcact caacttacgg caacgttgat gtgctgaaag cggcttgccg
    27901 tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca acggcgataa
    27961 agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga cgccacgaat
    28021 gatgccgggt gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt
    28081 cgataagggt ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg
    28141 gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa
    28201 cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg
    28261 cttgtaaaga ctacaaagat ttgatgccag aagtcagctt ccgccgcatt tatgaatatg
    28321 ctggtggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc
    28381 tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg
    28441 cgatgcataa acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc
    28501 gctactgcca tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata
    28561 tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg
    28621 aatcttgtcc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg
    28681 gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga
    28741 tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga
    28801 aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc
    28861 gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgcttaa aggcgacctg
    28921 cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc
    28981 attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt taactcgctc
    29041 aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgct
    29101 gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcacctgc attgcgtacg
    29161 ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg
    29221 tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc
    29281 ctgacgatgt tcatgggcgg cccgttactg ggttacctgt tgctgagtct ggctggcgtc
    29341 gatggctggg cgatgcgtct gctgccagcc atttctgtac tggcactggt ggtgagtggc
    29401 gtgatgtcag tgatgcaggg cacagagctg gcaactattc atagttctgt gcagcaggcc
    29461 gcagcgctgg tgccggacta tggtgcgctg atgtcctggc gaatcgtgct tttggccgtt
    29521 gccctgtgct tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta
    29581 tccgtctcat tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt
    29641 ttgcatatga ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc
    29701 ccggttctct tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa
    29761 tcaggaatgt ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg
    29821 tgcagctcgc aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga
    29881 tcccatgccg aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaag
    29941 acatcaaacc cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc
    30001 acaccggcaa taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg
    30061 ttatcccagg cgttaatatt gccagggcga gcaatgtaag gtgcatcggg gaattgtgct
    30121 ttcagctctg gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt
    30181 ggtaagttga aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg
    30241 ggttcgatat cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacggca
    30301 gcatcatttt tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca
    30361 gtggtacgtc tgaaatcggt cagacgcgat gcattgctct gaaagcatag acgggaaata
    30421 tgagtttgct gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga
    30481 tcaataaaaa caatcagatg agattaatca tctcttcagc attagcgtta tgaatgagta
    30541 atataaatcg ataataccgc cgctgcgata tgcgcgtatt ttcacccatt gacaatgttt
    30601 tttgcggtgg catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac
    30661 gtatacccgg cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc
    30721 ggtgttaaat acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt
    30781 aggcgttgtc agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta
    30841 tgtcattaaa cgcattggct ttaaccgcag ctattatctg gcctccttca tttttgccgc
    30901 tggctgtgca ggccttggcc tgatgattgg tttctggagc tggttggcgt ggcgttttgt
    30961 cgcgggcgtc ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg
    31021 gacgtcacgt aaccgtgggc gtttgcttgc tgcgtatatg atggtttatt acgtgggaac
    31081 atttttaggc cagttactgg tcagcaaagt ctcaaccgag ctgatgagcg tattgccgtg
    31141 ggttacaggt ttgacgttgg cagggatctt accgctgttg tttacgcgtg tcctgaatca
    31201 gcaggctgaa aaccatgatt cgacgtcaat tacgtcaatg ctaaaactcc gtcaggcgcg
    31261 gcttggcgtg aatggctgca ttatctcagg aatcgtgctg ggatctctat atggcctgat
    31321 gccgctgtac ctcaatcaca aaggggtgag caatgccagc attggtttct ggatggcggt
    31381 actggtcagt gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg
    31441 tcgactgctg gtgttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct
    31501 tagccaggcg gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata
    31561 tccggtggcg atggcctggg cttgtgagaa agttgaacat catcaactgg tggcgatgaa
    31621 ccaggcctta ctgttgagct ataccgtggg aagtctgctt ggcccgtcat ttaccgctat
    31681 gctaatgcag aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat
    31741 ctatttgctg atgctgcttc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta
    31801 aatgaattca agcaaagtgt gaacttactg tttcacactc tgcttttttg tttcttctat
    31861 ctgacttgct tttttccaaa ttttattcgt ttaaaaataa aatgtgcagc aggttataat
    31921 tttgcatttc gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat
    31981 gcccctttaa ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg
    32041 cgatggtaca acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt
    32101 aataactatg ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc
    32161 tatttcacac cttatgcact aattgttggt cagttaggct cgaccttcaa agatgggaag
    32221 ggcggggtca gtacctggat taaacacacg atgggacccg gattggctta cctcgccgcg
    32281 tggacctact gggtggtgca tattccctat ctggcacaaa aaccccaggc aatactgatt
    32341 gcgctcggtt gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg
    32401 ttacaggggt taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg
    32461 aaatcgctga aaatcgtcgg ttctgtggca gggattgcga tgtttgttat gtcgctcctg
    32521 tatgtggcga tggcggtaac cgcgcctgcg attactgaag tgcatattgc gaccacaaac
    32581 attacctggg aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg
    32641 ctggttttcg cggttggcgg agctgagaag atttctcctt acgttaatca aacgcgcaac
    32701 ccaggaaaag aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc
    32761 attctgggct cactggcgat ggggatgatg tttgactcgc gtaatatccc ggatgactta
    32821 atgactaacg gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat
    32881 actttaatgg tgatttacgc cattgcgaat accctgggac aagtagctgc gctggtattc
    32941 tcgattgatg ccccgcttaa agtgctatta ggcgatgctg acagcaaata tattccagcc
    33001 agtttatgtc gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggtg
    33061 ctggtggcga tcctgattat gctgccgact ctcggcatcg gtgatatgaa caatctctat
    33121 aaatggctgt tgaatcttaa ctcggtagtg atgccgctgc gttatttatg ggtatttgtt
    33181 gcatttattg cagtcgttcg cctggcgcag aaatatacac cagagtatgt ctttattcgt
    33241 aataagcctc tggcaatgac cgtcgggatt tggtgttttg cctttactgc ctttgcctgt
    33301 ttgacgggga tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg
    33361 ctgaatgttg caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg
    33421 cgtaaagcga atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc
    33481 cgtttttaca taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta
    33541 atacatttgg aagctgtgct gtaagggcgt tagaaataga gctctgaatt atttatgcag
    33601 cgcggaataa aaaagcggca actcaataaa gttgccgctt tacggggaaa ttagaacatt
    33661 accttatgac cgtactgctc aagaatacct ttcacgcgtt ccatggtttc tttcttcggt
    33721 ggtttaacac catcgagttt gtattcttca cccatcgcca cccatttgtg tttgcccagt
    33781 tcgtggtagg ggagaagttc gattttctca acgttaccca tatcacgggt aaattcaccg
    33841 aggcgatgcg ctgaatcgtc atcgtcagac cagcctggga caacaacgta gcggatccac
    33901 accttcacat ttttattcgc cagatatttg gcgaactcca gcgtgcggtg gttggaaact
    33961 ccaaccagat tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg
    34021 gttacttcca gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtgtcc
    34081 agacaggtat gaatgccttc ttttttgcag gcgcggaacc agtcacgaac aaactcagct
    34141 tgcaggattg cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg
    34201 cgataggtca ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgtg
    34261 tcccaggtgt cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg
    34321 ataaagcgaa tacctgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca
    34381 ataactgaca ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag
    34441 ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa
    34501 aaggctcacg tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact
    34561 gtacgatttc agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct
    34621 gctgctgttc tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca
    34681 gctgcggata tttttccgga ttttccatcg cgtcgagcag catttcacgg ttcatcacgt
    34741 taacgttcag gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca
    34801 gaccagccag gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag
    34861 agaaggtgta ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca
    34921 gagacgctac agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg
    34981 gcgcgccagc acgacgaccg tctggggtgt tacccgtttt cttaccatac acaacgttag
    35041 aagtgatggt cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa
    35101 ttttcttcat gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcgttgt
    35161 taccaaactg cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac
    35221 gaatcggttt aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac
    35281 cagcgatacc acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg
    35341 cttcgtagct gtacttgtcg tgcatgtagt gaatgatgtt cagtgcagtg atgtactgtt
    35401 tagccagcca gtccatgaag tgatccatgc gctccatcac ttcatcgtag ttcaggagat
    35461 cgcctttgat cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc
    35521 cgttgattgc gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt
    35581 gtttaccaac gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt
    35641 ccggacgcat caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag
    35701 cggcgaattt cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt
    35761 ccggagacgg acccatggtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca
    35821 gggtacgacc gtcgaggccc ataccaccga tagattcggt tgcccagatc gggtcgccag
    35881 agaacagttc atcgtactcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca
    35941 ggtggtcaac catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt
    36001 cgatgtacac atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag
    36061 acttaacagc agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag
    36121 ccggaccaga gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc
    36181 ggtgctgttc agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt
    36241 ccagatcagc ctgcagagag gtgaactgag cgtatttgtc tttcatcagg tagtcgatac
    36301 cgtacagcgc aacgcgacgg tagtcaccga tgatacggcc acggccataa gcatctggca
    36361 gaccggtcag aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca
    36421 cgccctggtt gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt
    36481 cgcggttgta cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag
    36541 cacgtttcag cggagcttca gtctgcagac caacaacttt ttccaacgct ttgttgatgt
    36601 agccagcgtc gtgagaggtg atggtggaag caacagcggt gtcgaagtca actggcgcgt
    36661 gagtgcggtt ttccagttta acgccttcca ttactttgtc ccacagggtg gtggtcgctt
    36721 cagtagcgcc agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga
    36781 agtcacggac gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg
    36841 ctaacttttc attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta
    36901 ctgcgtactt cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg
    36961 tcaacccaac caacaaacca ccgccgataa tattaccgat cgtaaccgga atcaggttat
    37021 cagtgatgaa gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg
    37081 tccagaattc cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt
    37141 ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accatgataa
    37201 acgctttgtc catcaggctg cggccagaat aactcatcca tactgccaga cataccatca
    37261 ggtttgccag gataccaagg cagacggcct caataaaagt atggtgcact ttgtggtcgg
    37321 cggtttgtag gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc
    37381 acattaaaag tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt
    37441 ttttagccaa ctgaccccag gtgatgcgcc cactcgcctt agcaacaaca atcaacactg
    37501 tggaagtaaa gagatcggct ccgcagacaa cacaaagaat cagtcccaga gagaagcaaa
    37561 tgccgccaac cagttttgcc atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga
    37621 tatagaagac gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct
    37681 taagcggatg tttcgttgct ttatagacac ccgcctcttc ggccactttg gccattgcag
    37741 caggaagtaa aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc
    37801 ggcgacgaga tactaacaaa gcattataga tgag
//
LOCUS       DAZFKY010000023        37815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFKY010000023 DAZFKY010000000
VERSION     DAZFKY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            116..2359
                     /gene="pdeF"
                     /locus_tag="ACHKCL_001065"
     CDS             116..2359
                     /gene="pdeF"
                     /locus_tag="ACHKCL_001065"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5762080.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2398..3939)
                     /gene="ppx"
                     /locus_tag="ACHKCL_001066"
     CDS             complement(2398..3939)
                     /gene="ppx"
                     /locus_tag="ACHKCL_001066"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5762081.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3944..6010)
                     /gene="ppk1"
                     /locus_tag="ACHKCL_001067"
     CDS             complement(3944..6010)
                     /gene="ppk1"
                     /locus_tag="ACHKCL_001067"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5762082.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6181..6819)
                     /gene="purN"
                     /locus_tag="ACHKCL_001068"
     CDS             complement(6181..6819)
                     /gene="purN"
                     /locus_tag="ACHKCL_001068"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5762083.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6819..7856)
                     /gene="purM"
                     /locus_tag="ACHKCL_001069"
     CDS             complement(6819..7856)
                     /gene="purM"
                     /locus_tag="ACHKCL_001069"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5762084.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8181..8807
                     /gene="upp"
                     /locus_tag="ACHKCL_001070"
     CDS             8181..8807
                     /gene="upp"
                     /locus_tag="ACHKCL_001070"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5762085.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8893..10182
                     /gene="uraA"
                     /locus_tag="ACHKCL_001071"
     CDS             8893..10182
                     /gene="uraA"
                     /locus_tag="ACHKCL_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5762086.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10277..10978
                     /gene="hda"
                     /locus_tag="ACHKCL_001072"
     CDS             10277..10978
                     /gene="hda"
                     /locus_tag="ACHKCL_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5762087.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11116..11475)
                     /gene="arsC"
                     /locus_tag="ACHKCL_001073"
     CDS             complement(11116..11475)
                     /gene="arsC"
                     /locus_tag="ACHKCL_001073"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5762088.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11496..12959)
                     /gene="bepA"
                     /locus_tag="ACHKCL_001074"
     CDS             complement(11496..12959)
                     /gene="bepA"
                     /locus_tag="ACHKCL_001074"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5762089.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13172..14233
                     /gene="yfgO"
                     /locus_tag="ACHKCL_001075"
     CDS             13172..14233
                     /gene="yfgO"
                     /locus_tag="ACHKCL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5762090.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14271..15119)
                     /gene="focB"
                     /locus_tag="ACHKCL_001076"
     CDS             complement(14271..15119)
                     /gene="focB"
                     /locus_tag="ACHKCL_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO5762091.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15141..17153)
                     /gene="hyfR"
                     /locus_tag="ACHKCL_001077"
     CDS             complement(15141..17153)
                     /gene="hyfR"
                     /locus_tag="ACHKCL_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO5762092.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17183..17596)
                     /gene="hyfJ"
                     /locus_tag="ACHKCL_001078"
     CDS             complement(17183..17596)
                     /gene="hyfJ"
                     /locus_tag="ACHKCL_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO5762093.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17589..18347)
                     /gene="hyfI"
                     /locus_tag="ACHKCL_001079"
     CDS             complement(17589..18347)
                     /gene="hyfI"
                     /locus_tag="ACHKCL_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO5762094.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18344..18889)
                     /gene="hyfH"
                     /locus_tag="ACHKCL_001080"
     CDS             complement(18344..18889)
                     /gene="hyfH"
                     /locus_tag="ACHKCL_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO5762095.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18899..20614)
                     /gene="hyfG"
                     /locus_tag="ACHKCL_001081"
     CDS             complement(18899..20614)
                     /gene="hyfG"
                     /locus_tag="ACHKCL_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO5762096.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20604..22184)
                     /gene="hyfF"
                     /locus_tag="ACHKCL_001082"
     CDS             complement(20604..22184)
                     /gene="hyfF"
                     /locus_tag="ACHKCL_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO5762097.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22189..22839)
                     /gene="hyfE"
                     /locus_tag="ACHKCL_001083"
     CDS             complement(22189..22839)
                     /gene="hyfE"
                     /locus_tag="ACHKCL_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO5762098.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22851..24290)
                     /gene="hyfD"
                     /locus_tag="ACHKCL_001084"
     CDS             complement(22851..24290)
                     /gene="hyfD"
                     /locus_tag="ACHKCL_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO5762099.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24307..25254)
                     /gene="hyfC"
                     /locus_tag="ACHKCL_001085"
     CDS             complement(24307..25254)
                     /gene="hyfC"
                     /locus_tag="ACHKCL_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO5762100.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25265..27283)
                     /gene="hyfB"
                     /locus_tag="ACHKCL_001086"
     CDS             complement(25265..27283)
                     /gene="hyfB"
                     /locus_tag="ACHKCL_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO5762101.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27283..27900)
                     /gene="hyfA"
                     /locus_tag="ACHKCL_001087"
     CDS             complement(27283..27900)
                     /gene="hyfA"
                     /locus_tag="ACHKCL_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO5762102.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28153..28623)
                     /gene="bcp"
                     /locus_tag="ACHKCL_001088"
     CDS             complement(28153..28623)
                     /gene="bcp"
                     /locus_tag="ACHKCL_001088"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5762103.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28623..29195)
                     /gene="gcvR"
                     /locus_tag="ACHKCL_001089"
     CDS             complement(28623..29195)
                     /gene="gcvR"
                     /locus_tag="ACHKCL_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5762104.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29341..30219
                     /gene="dapA"
                     /locus_tag="ACHKCL_001090"
     CDS             29341..30219
                     /gene="dapA"
                     /locus_tag="ACHKCL_001090"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5762105.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30236..31270
                     /gene="bamC"
                     /locus_tag="ACHKCL_001091"
     CDS             30236..31270
                     /gene="bamC"
                     /locus_tag="ACHKCL_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5762106.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31483..32196
                     /gene="purC"
                     /locus_tag="ACHKCL_001092"
     CDS             31483..32196
                     /gene="purC"
                     /locus_tag="ACHKCL_001092"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5762107.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32364..33227
                     /gene="ypfJ"
                     /locus_tag="ACHKCL_001093"
     CDS             32364..33227
                     /gene="ypfJ"
                     /locus_tag="ACHKCL_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5762108.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33242..35257
                     /gene="tmcA"
                     /locus_tag="ACHKCL_001094"
     CDS             33242..35257
                     /gene="tmcA"
                     /locus_tag="ACHKCL_001094"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5762109.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35331..36029
                     /gene="ypfH"
                     /locus_tag="ACHKCL_001095"
     CDS             35331..36029
                     /gene="ypfH"
                     /locus_tag="ACHKCL_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5762110.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36110..36310)
                     /gene="ypfN"
                     /locus_tag="ACHKCL_001096"
     CDS             complement(36110..36310)
                     /gene="ypfN"
                     /locus_tag="ACHKCL_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5762111.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36338..37465)
                     /gene="dapE"
                     /locus_tag="ACHKCL_001097"
     CDS             complement(36338..37465)
                     /gene="dapE"
                     /locus_tag="ACHKCL_001097"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5762112.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37469..>37815)
                     /gene="yffB"
                     /locus_tag="ACHKCL_001098"
     CDS             complement(37469..>37815)
                     /gene="yffB"
                     /locus_tag="ACHKCL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5762113.1"
                     /translation="YGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNGFINE
                     LGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPMLLGF
                     SDSSYQKFFHEV"
BASE COUNT         9179 a         9818 c         9973 g         8845 t
ORIGIN      
        1 tatgacactt agaatcaatg ctctctatag tgatgaataa tcatcattcg aagtcaggtg
       61 ggatgcctgt ctgaatacac ctccttcagg atgtggggga ttctgctgag catctatgaa
      121 actgaatgca acttatataa aaatacgtga taaatggtgg gggcttccgc tgttcctgcc
      181 ttctttaatc ttgcccattt tcgcccacat taatactttc gcgcatattt cttccggtga
      241 ggtttttctc ttttatctgc ctctggcact gatgatcagc atgatgatgt ttttcagctg
      301 ggcggcattg ccagggatcg ccttagggat ttttgtccgc aaatatgcag agctgggttt
      361 ttacgaaacg ctctcattaa cggctaattt tattatcatt atcattctct gttggggcgg
      421 ttacagggtc tttactcccc ggcgtaacaa cgtttcacat ggtgataccc gtttaatttc
      481 ccagcgtata ttctggcaga ttgtgtttcc tgcaacgctg tttctgatac ttttccagtt
      541 tgctgcattt gtaggattac tggcgagcag agaaaatctg gttggcgtca tgccttttaa
      601 tctcgggacc ttaatcaatt atcaggcctt actggtgggc aatctgattg gtgtcccgct
      661 gtgctacttc atcattcggg tagtgcgaaa tccgttttat ttacgtagtt attattcgca
      721 attaaaacag caggttgatg tcaaagtcac caaaaaagag ttcgcactct ggctgctggc
      781 attaggtgct ttactgttgc tgttatgcat gccgttaaat gaaaaaagca caatttttag
      841 caccaattat accttgtcat tattgctgcc cctgatgatg tggggagcga tgcgctatgg
      901 ttataagctg atttcgctgc tctgggcggt cgtgttgatg atcagcatcc acagctatca
      961 aaattacatt cccatttatc ctggctatac cacgcagctg accataacct cctccagtta
     1021 tctggtattc tcttttattg tcaattatat ggctgtactg gcaacccgtc agagaacggt
     1081 agtcagacgc attcagcgcc ttgcgtatgt ggacccggtg gttcatctgc caaatgttcg
     1141 cgccctgaat cgcgcgttgc gtgatgcccc ctggtctgcg ctttgttatt tacgcatccc
     1201 tggcatggaa atgctggtta aaaactatgg catcatgctg cggattcaat acaagcaaaa
     1261 actttctcac tggctgtcac ccttgctgga accaggtgaa gatgtttatc agctttcggg
     1321 taacgatctc gcgctgcgac tgaatacaga atcgcaccag gagcgcatta ccgcactgga
     1381 tagccatctc aagcaatttc gtttcttttg ggatggcatg ccgatgcaac cgcagattgg
     1441 cgtcagttac tgctatgtgc gctcgccagt gaatcatatc tacctgctgc tgggagagct
     1501 aaatacggtc gccgaacttt ccatcgtgac caacgccccg gaaaatatgc agcgtcgcgg
     1561 ggcaatgtat ttgcaacgcg aattgaaaga taaagtcgcg atgatgaatc gactacagca
     1621 ggcgctggaa cacaaccatt ttttcctgat ggcccagccg attaccggta tgcgtggtga
     1681 tgtttaccat gaaattcttc tgcgcatgaa aggtgagaat gatgaactaa tcggccccga
     1741 tagcttcttg ccggtcgcgc acgaatttgg tttatcgtcg agtatcgaca tgtgggtcat
     1801 tgagcatacg ctgcaattta tggctgaaaa cagagcgaag atgcccgctc accgttttgc
     1861 tattaatctg tctccaacct cggtatgtca ggctcgtttt cctgttgaag tcagtcagtt
     1921 gctggataaa tatcagattg aagcgtggca acttattttt gaagtcaccg aaagtaatgc
     1981 tctgaccaat gttaagcagg cgcaaatcac cttgcagcat cttcaggaat taggctgcca
     2041 gattgcgatt gatgatttcg gcaccggcta cgccagctat gcgcggctta aaaatgtgaa
     2101 tgccgatctg cttaaaattg acggcagttt tatccgcaat attgtgtcaa atagtctgga
     2161 ttatcagata gtggcatcga tttgccacct ggcgcgaatg aagaaaatgc gggtagtggc
     2221 agagtacgtt gaaaacgaag agatccgcga ggcggtgctc tctttgggga tcgattatat
     2281 gcagggttat cttattggta agccgcaacc gttaattgat acgctgaatg aaatcgaacc
     2341 cattcgcgaa agtgcctgaa taatgcgggc cgacatttct cgtcggcccg caaagcatta
     2401 agctgcgatt tcaggtgtac tttcttcttc aattttcaac cgccagccag ccacgccttc
     2461 ccagtattct tgctcctttt ccagatcaag cagtaccagc gcattctgac taaaccagtc
     2521 atgcgggaaa cgcagtgtcc agtgactgtc atcagtaatc agcgtcaatg ttggcggtgt
     2581 ggtggttgcc tgacgctgat tgttgagtaa tacgccaagg cgcaatagct gaatcagtgg
     2641 caggaactgt ttcttcttaa acaaggtaaa gcgcggcaga tcgtcgagtt taatcgcttt
     2701 acggtgatag cgcaccagtg tcgccatcat cagctgctgt tcctgattaa aacccggcaa
     2761 gtcgctgttt tgtagaatat aagcggagtg acgatgcaaa ccgctgtggt tgatattcaa
     2821 cccgacctca tgcagcatgg cggcccatcg cagtagcgcc tccagttgcg gatgcgccag
     2881 cttcggttgc tgttcccgcc actgttcgta catttgcata gtggtatcca gcacccgtcg
     2941 ggcctgttcg ctgtcgatgt gatactggtt ggcgaggctg ctggcggtgc gactacgcac
     3001 atcctgatga cggaaacgtc cttccatttc atacagtacg ccttcgcgta acgccccgtc
     3061 agaaaggcgc agttcacgga tggctaaagc atcaaacaca ccgcataaaa tcgctagtcc
     3121 aggaacgaag actgttttcc gctcttcgga aagacctggt aaactcagcg atgcgaaatt
     3181 acggtgccgt aaaacttctt ttaccagttt ttccagacgt tccggggtaa ttatcccgtc
     3241 tttctcgccc atttccatta acacttcatg ggcggctttt atggtaccgg aagcgcccat
     3301 tgccacgttc cagccctgaa tacgaaattg ccaggttaaa gtttccagtt tttgtgctgc
     3361 cgccatgcga gcgcgctgaa aattctcttt attgatgacc ccgccaggga aataaagctg
     3421 ggcaaagctg acacacccca tccggcggct ttcaacgaga atcggttcga aattttcacc
     3481 aatcaccagt tctgtagaac cgccgccaat atcaataacc agtttgcgac ctttttccgg
     3541 ttgggtatgt tccacgccca taaaaatcag acgggcttct tcattaccgg aaataatttc
     3601 aatcgggtag ggaatgacct tttccgcacg tttcagaaag tcagtggcgt tcagcgcctg
     3661 acgcagcgta tgggtaccaa ctatacagac gctggcagga gaaaaccctt gtagccgttc
     3721 ggcaaacagc gacagacagt tcaaaccgcg cgtcattgcc tcttcactca acatattatc
     3781 tggccccagg ccgtccgcca gatgcacccg ctgtttcagg cggccaataa tctgcatggc
     3841 accatctacc acacgggcta tgaccatgtg aaaactgttt gaaccaagat cgaccgcagc
     3901 aaactcctgc ggacgagggg atttatcgtg tattggcata gggttattca ggttgttcga
     3961 gtgatttgat gtagtcataa atcgccaact gcgcccgtac tttgcggcga ttgccgcggg
     4021 gaacgtagcg attactgagt tctttatcga tataacgtgc tttgaccgta tcgctgaaca
     4081 atatgtcgat gatgtccagc acccgctgct tcaggcgcgg atcgagcagc ggcgtcgcca
     4141 cttcaatacg ataatcaata ttgcgcgtca tccagtcggc ggaagaaagg tagacctttt
     4201 tatcgccgcc attttcaaaa atataaaccc ggtcatgttc aaggtaacgg tcaacaatac
     4261 tgatggcacg aatgttgtcg ctaatgcctt ccagattggg gatcagcgaa cacattccgc
     4321 gaaccagcag attaaccggt acgccggagc tggaggccgc atacagacga tcaaccaggc
     4381 ctttatcgac aaggttattt agcttcaggg tgataccact gggcagccct tgctgcgcgt
     4441 tggcgatctc gcggtccacc atttcataca acaggcggcg ggagttttgc ggcgacacca
     4501 ttaaataatc aaatgtcacc ggacggtatg ggttttcaat aaagttaaat acccgccgta
     4561 cttcgttggt gatgcgcgca tcggcggtca gcaacgaata gtcagtataa agacgcgcgg
     4621 ttttttcgtt aaagttcccg gtcccgatgt gtgcataacg caccacttca ccgttttctt
     4681 tacgtgaaat cagaaacagt ttggcgtgaa ttttcagccc cggcgcagag aagataacgt
     4741 gcacgcctgc ttcggtcagg cgcttcgccc agtgaatgtt ggcttcttcg tcaaaacgtg
     4801 cctgtagctc aaccaccacg gtgactttct taccgttgtg tgcggcgtgg atcatcgagt
     4861 cgatgatgcg tgaatctttt gccacacggt aaatgttgat tttaatcgcc agcacgctcg
     4921 gatcgaacga ggcctgacgc agcagttcca gcacatgctc aaaggtgtga taaggataat
     4981 agagcaacac atcgcgttcg cgaatggcat caaaaccatt gcggaactgg gctttatcaa
     5041 accaaatatg gcgtaaacgc ggcagtggtt tgttcaccag attggctttg ccgacattgg
     5101 ggaaattaat aaagtcttta aaattatgat aacgaccgcc ggggacgatg gagtcgtagc
     5161 gggaaatagt cagtttttcg cgtaacactt caaccagcgc attgggcata tcgcgctgat
     5221 aaacaaaacg caccggctca gcagttaaac gctgcttgag actggaagac atcaactcca
     5281 tcaggctggc ttccatctca tgcactaaat cgtattcggc atcgcgggtc atcttcattg
     5341 aataggcatt cagcgcgtca taatcaaaga agcctttgaa aatatcatca aggcagtaac
     5401 gcagaatgtt atccagaaga atcattggct tgcgtcgacg cggcgcttct ggcggtaaat
     5461 tcacaaagcg cggcacttta tctgatggga tctccagaag cgcgtaacgg atggtatcgc
     5521 cacggataat ttccaccgcc agataggtgt aatcatcttt caggaactgc actaagtcag
     5581 tgtcaggatt gattaaaatc ggcgtaatgt gctgacgcag atactgctta aaataatgac
     5641 gcagccagtt ttgttgattg acggagagct ggcgttcatt aatcaggaag atctggttgc
     5701 gcgccatctc cagcaatagc tcgttgtaga ggccgtcgaa ttcctgatcg gctttcagca
     5761 cccgggactg aattttgccc agtaaatggc gggaatgaga gttggagcct tgttcttcgc
     5821 taataatgat gcgtcgcttc agttcagcga agcggacttt atagaactca tcaaggttat
     5881 tggaatagat ccccaggaaa cgcatccttt caatcagcgg gttagatttg tccgccgctt
     5941 cctgaagcac gcgttcattg aacgataacc aactgagctc tttttcgatg tatagctttt
     6001 cctgacccat taccactttt actccgtttt attcacggga cactgcctgg atattatggc
     6061 gagcaatatt ggcagatgtc caactatgcc agaaaagggg gaaaaaaact gctggagctt
     6121 gcgatttaca ggtgaattga aaaacgccag cggcagagct ggcgctttaa ctacggggca
     6181 ttactcgtcg gcagcgtagc cctgcggcgg cagacgttga ccatccagcc acgcggcgtt
     6241 ttcgtgcatt ttcagacgac catcggcaaa ccagctaatc accagtggat aaatggcgtg
     6301 ttcctgggtt tgcacgcggg cggtgacgtc atcctccgta tcaccagcaa ataccgggac
     6361 tttcgcctgt aaaataaccg ggccaccgtc cagttcatcg gtgacgaaat gcaccgatgt
     6421 accgtgctct tcatcgccat tttccagcgc ctgacgatgg ggatgtaatc cgggatattt
     6481 cggcagcaga gaagggtgaa tgttcagcaa acgcccggca tagtgggaga caaatgccgg
     6541 gctgagaatg cgcataaaac cagccagcac gaccacatcg ggtgcgtaca tgtcgatttc
     6601 atgaatcaac tcccggtcat aggcttcacg actgtcaaac gcgctggcga tgagcgtatg
     6661 cgttgcaata cccgcctggc gggcgcgttc aaggccgaac gcgtcggcct tattgctgaa
     6721 aactgcccgt acggtgcctt taattttgtt ggttttacag gcgtcaataa ttgcctgcaa
     6781 attacttccg ttgccggaaa taagcaccac aatattcatt attcgataac cacgcgttgt
     6841 tcggaatcag aggctttgat gataccgatt ttccacgcgt tttcaccgtt ggcattgagc
     6901 agggcgaggg ctttgtccac ttccggagca ggcagggcaa taatcatccc gacgccgcag
     6961 ttgaaggtgc gatacatttc atggcgctca acgttaccag ccgtttgcag ccagttgaac
     7021 acttccggcc actgccagga ggattcatca atcactgcct gggtattatc tggcaatacg
     7081 cgcggaatgt tttcccaaaa gccgccgccg gtcaggtgcg caatggcatt aacatcgacc
     7141 ttttcaatca actccagcac tgacttcacg taaatgcggg tcggtgccag cagatgatcg
     7201 gctaatggct taccatcaag ttcggtggtt tgcggatcac aaccgctgac ttcaagaatt
     7261 ttgcgcacca gcgaatagcc gttcgagtgc ggaccgctgg aaccgagtgc aatcagcaca
     7321 tcgccgtcgc tgactttaga gccgtcgatg atttctgatt tttctaccac gcccacgcag
     7381 aaacccgcga catcgtaatc ctcaccgtga tacatccccg gcatttctgc cgtttcgcca
     7441 cccaccagtg aacagcccga ttgcagacaa ccttccgcaa tgccgctgat caccgctgaa
     7501 gcggtatcaa catccagttt tccggttgcg taatagtcga ggaaaaacag cggttctgca
     7561 ccttgcacca ccaggtcatt aacgcacatg gcgaccagat caataccaat ggtgtcgtga
     7621 cgttttaagt ccattgccag acgcagcttg gtacctacgc cgtcagtgcc ggaaaccagc
     7681 acgggttcac gatatttttg cggcaatgca cacagcgcac cgaagccgcc cagaccgccc
     7741 atcacttccg gacgacgcgt tttcttcact acgcctttga ttcttccaac cagagcatta
     7801 cccgcgtcaa tatcaacacc ggcatctttg tagctaagag aggttttatc ggtcactgct
     7861 tgggtcccca cgcgttactt gcggtagaaa aataaaattc ggcgcaattc taacagggaa
     7921 agcaaacgtt tgcgagactg ctttacacaa cctttttgca cgtcttttcc ccaggcgcgc
     7981 ggcgaaagaa gacttgtgcc agggcaaagg ttagttttcg gatggaataa tcttctttca
     8041 taaccatttg aatataaaat aactttatct caaaccgtta tcattttgac taaagtcaac
     8101 gaaaataata ttgccgcctt gaagaaagga ggtataatcc gtcgattttt tttgtggctg
     8161 cccctcaaag gagaaagagt atgaagatcg tggaagtcaa acacccactc gtcaaacaca
     8221 agctgggact gatgcgtgag caagatatca gcaccaagcg ctttcgcgaa ctcgcttccg
     8281 aagtgggtag cctgctgact tacgaagcga ccgccgacct cgaaacggaa aaagtaacta
     8341 tcgaaggctg gaacggcccg gtagaaatcg accagatcaa aggtaagaaa attaccgttg
     8401 tgccaattct gcgtgcgggt ctggggatga tggacggtgt gctggaaaac gttccgagcg
     8461 cgcgcatcag cgttgtcggt atgtaccgta atgaagaaac gctggagccg gtaccgtact
     8521 tccagaaact ggtttctaac atcgatgagc gtatggcgct gatcgttgac ccaatgctgg
     8581 caaccggtgg ttccgttatt gcgaccatcg acctgctgaa aaaagcgggc tgcagcagca
     8641 tcaaagttct ggtgctggta gctgcgccag aaggtatcgc tgcgctggaa aaagcgcacc
     8701 cggacgtcga actgtatacc gcatcgattg atcagggact gaacgagcac ggatacatta
     8761 ttccgggcct cggcgatgcc ggtgacaaaa tctttggtac gaaataaaga ataaaaataa
     8821 ttaaagccga ctttaagagt cggctttttt ttgagtaaag cgcctataac acataataca
     8881 gaggataata ctatgacgcg ccgtgctatc ggggtgagtg aaagaccgcc acttttacag
     8941 acaatcccgc ttagtttgca acatttgttc gccatgtttg gtgcaaccgt cctggtgccc
     9001 gtcttatttc atattaaccc ggcgactgta ctgttattta acggtattgg aacgctgctg
     9061 tatctcttca tctgtaaagg gaaaattccg gcttatcttg gttccagctt tgcctttatt
     9121 tcaccggtat tgttactgtt gccgttaggg tatgaagtcg cgctgggcgg ctttattatg
     9181 tgcggcgtgc tgttctgcct ggtttctttt atcgtgaaga aagcggggac cggctggctg
     9241 gacgtgctgt ttccacctgc ggcaatgggc gcaatcgttg ccgtcatcgg tctggagctg
     9301 gcgggcgtag ctgccggtat ggcgggttta ctcccggctg aagggcaaac gccagactcc
     9361 aaaaccatca tcatctctat taccaccctg gcggtcacgg ttttaggttc cgtgctgttc
     9421 cgtggtttcc tggcaattat cccgatttta attggcgtgc tggtggggta cgcgctctct
     9481 ttcgcaatgg gaattgtcga taccacgccg attattaatg ctcactggtt tgcgctgcca
     9541 accctctata cgccgcgctt cgagtggttt gccattctga ctattctgcc agcggcgtta
     9601 gtggttattg ccgaacacgt agggcacctg gtagtaacgg ctaatatcgt caaaaaagat
     9661 ctgctgcgcg atccaggtct gcaccgttcg atgtttgcta acggcttgtc gaccgtgatc
     9721 tccggcttct ttggctccac gccaaacacc acctacgggg aaaacattgg cgtgatggcg
     9781 atcacccgtg tttacagtac ctgggttatc ggcggggcgg cgattttcgc tatcctgctt
     9841 tcctgcgtcg gtaaactggc tgccgctatc cagatgatcc cattgccggt gatgggcggc
     9901 gtttcgctgc tgctttatgg tgtcatcggt gcttccggta ttcgtgtttt gatcgaatcg
     9961 aaagtggatt acaacaaagc acagaacctg atcctgactt ccgtgatttt gatcatcggc
    10021 gtcagtggcg cgaaggtaaa cattggcgcg gcggagctga aaggtatggc gctggcgacc
    10081 atcgtcggta tcggcttaag tttgattttc aaactgatta gtgtgctgcg ccctgaagaa
    10141 gtggtgctgg acgcagaaga cgcggatata acagacaaat aatgtaccga ccgggcagtg
    10201 ttgctgcccg gttcaaacat catgggattc tgtggtaaac ttctcgcgat tttgtgaaat
    10261 cctggttgag gtatctctga acacaccggc acagctctct ttgccacttt atcttcctga
    10321 cgacgaaacc tttgcaagtt tctggccggg ggataactcc tctttactgg ccgcgctgca
    10381 aaacgtgctg cgtcaggaac atagcggtta catctatctc tgggcacgcg aaggcgcggg
    10441 gcgcagccat ctgctgcacg cggcttgcgc ggaattgtcg cagcgtggcg atgcggtggg
    10501 ctatgtcccg ctggataaac gcacctggtt tgttccggaa gtgctcgacg gtatggagca
    10561 tttgtcgctg gtctgtatcg acaacattga gtgtattgca ggcgatgagt tgtgggagat
    10621 ggcgattttc gatctctaca atcgaattct ggaatcgggc aaaacacggt tgttgatcac
    10681 cggcgatcgt ccaccgcggc agttgaatct gggattaccg gatctcgcgt cgcgactcga
    10741 ctgggggcag atctacaaat tgcagccact ttctgatgaa gataagttgc aggcgctaca
    10801 gttacgcgcg cgtttgcgtg gttttgaact gccggaagat gtggggcgtt tcttgctgaa
    10861 gcggctcgac agagaaatgc gcacgctatt tatgacgttg gatcagttgg atcgtgcgtc
    10921 gattaccgcg caacgtaagc tgaccattcc gtttgtgaaa gaaattctga agttgtagat
    10981 aaggtgttta ttgtcggatg cggcgcgaac gccttatccg aactacgcga agttaatcca
    11041 tagaattcgt aggccggata agacgtggca agcgtcgcat ccggcaatgt gcttcaacta
    11101 gcgatggagc acatcttaac ccacgatttc cagtacctgc tccggtggac gaccaatccg
    11161 cgctttgcca ttcgcgacca caatcgggcg ctccatcagc ttcggattat ccaccattgc
    11221 ctgaatcagc gcctcttcgc taagcgaact gtcagccagg ttcagttctt tataaagatc
    11281 ctctttctgg cgcatcaatt cacgggcgct attcatcccc agcattttca gcaaatcgcg
    11341 cagcgttgcc gcatcggcgg gtgtctcaag gtagagcacc acttctggct ccacgccgtt
    11401 ttctttcagc aggttcagcg tttcccggct ctttgaacag cgtgggttat ggtagatttt
    11461 tacctgtttg gtcattgttc ttcctttaat gcgaattaca tcttggtata aggcttaaag
    11521 cgttcctgca gctggcgcaa ctggtcgatg cgcgcatcgt aacgcgcttg ttgcaggctg
    11581 cctaatttca cctgcgaact ggcgctactc aacagcgaaa tggcctgatc gagtcgtccg
    11641 gcgagcgcat aaccttctgc tcgcgcagcc agctcctgat cgcggttatt tagcgcggct
    11701 tccgcctgtg ccagcaaatc ccagccgttg ctgtcatctt tattattaaa ggtgtagcga
    11761 ttaagaatat tggccgcttc ttgtggttga ccgccttgta gataagcgtt cgccaggttg
    11821 agctgcaaca caggattggt gcgcaaatcg cgggcatttt tcagacgatt gatcgcctca
    11881 ttggctttgt tttgcccaag atcgatatca gtagccagat cgagatacca tgcgttgcca
    11941 ggttctgccg ccagtaacgg ttgcagcgtt tttcgcgcct cgtcgtattt attggcttcc
    12001 atcgcctgta aagcacgacc atattgcgcc gctcgttgct gacgaacgtt tcctttcgcc
    12061 cattcatcca gcaaatcact ggtgagctgg ttacgtccgg aattatacat ccccagtgtg
    12121 cgcgctttcg ccagatagaa atcttccgac gactgcacca ccatcgggcg catctgatta
    12181 gcacggttgc gggcatctgc cagacgactt tccggcaacg ggtgagtcag taaaatttcc
    12241 ggcgggcgcg aggagtaacg cgcctgatcg agtaattttt cgaggaaggt tggcatcgcc
    12301 tgcggatcga atcccgagcg ttgcagcacc tgaataccaa tgcggtccgc ttcctgttca
    12361 ttttgctggg tgaaactgat catcccctga cgcgttcccg ccagtgtacc ggtcagcgcc
    12421 gccatccccg cctgcggact ggccatcgcc agtaaaatag aacctaacgc gccgacccag
    12481 gtcagcggcg cgttgcgctg ctgatcttcc atcgctcgcg ccaggtgacg ttgggtgacg
    12541 tgggagattt cgtgcgccat aactgaagcc agttgacttt cgttatcgga ataacggaac
    12601 agggcagagt gcagcaccac gttgccgcca aagaaagcaa aggcgttaat ttcgtcgttg
    12661 ttgatcagaa agaaatgaaa cggtgtctta accgaattgg catgcgaaac cagacgcatc
    12721 cccagcgaat taatatattg cgttaacagc gggtcattaa ttaacggcgc gctgccgcgt
    12781 agctggcgga cataatagtc gcccatctgc atttcctgac caatggaaag cgtgcttcct
    12841 gcggaggttc ccatatccgg caaggtgtct gcgctgtcgg caaatgccgg ggctacctga
    12901 ccaatggtca tagcagcaat gagggttgca accaggtttt ttttcaactg cctgaacata
    12961 acctctatcc tgtatttctg aagatagtca tttgaccgat actgtgaaat atcgttccca
    13021 ccgcgccgcc tttgagtgta acggcgaaga ttagcccact caaatgactt ttcagtttcc
    13081 tgaatccgtc ataaaaagcg aagtcttttt tgtgacattt cgatacaatt gcggatcgca
    13141 atcccgctct tgaggttcag ggaaggtctt tatgctcgaa atgttgatgc aatggtatcg
    13201 ccgccgtttt agcgacccgg aagcgattgc cttgctggtt attttagttg ccggatttgg
    13261 cattatcttt ttctttagtg gcctgcttgc tccgttgctg gtggctattg tgctggccta
    13321 tttgctggaa tggccaaccg tgcgcctgca atctattggc tgctcccgcc gctgggcgac
    13381 gtcgattgta ttggtggttt tcgtcggtat attgctactg atggcgttcg tggtactgcc
    13441 tatcgcctgg cagcagggca tctacttaat ccgcgatatg ccggggatgc tcaataagct
    13501 ttctgacttt gccgccacgt tgccgcgccg ctatccggcg ttaatggatg cgggcattat
    13561 tgatgcaatg gccgaaaata tgcgcagtcg gatgctgacc atgggcgatt cggtggtgaa
    13621 aatttccctc gcctcgctgg tcggtttgct gaccattgcc gtctatctgg tgctggtacc
    13681 attgatggtc ttcttcctgc tgaaagacaa agagcagatg ctgaatgccg ttcgccgggt
    13741 gctgccgcgt aaccgtggac tggcaggaca ggtgtggaag gagatgaatc aacaaatcac
    13801 caactatatc cgcggcaaag tgctggagat gatcgtagta gggatcgcca cctggctggg
    13861 attcttactc ttcgggctga actattcgct gctgctggcg gtgctggtcg gcttctcggt
    13921 acttattccg tacattggcg catttgtggt gaccattccg gtggttggcg tggcgctatt
    13981 ccagtttggt gcaggcactg aattctggag ctgtttcgcg gtgtatctga tcattcaggc
    14041 gcttgacggc aacctgttag taccggtgtt gttctccgaa gcggttaacc tgcatccgct
    14101 ggtgattatt ttatcggtgg tgatcttcgg tggtttgtgg ggattctggg gcgtattctt
    14161 cgccattcca ttggcgacgc tgatcaaagc cgtgatccac gcctggcccg atgggcaaat
    14221 agcgcaagaa taatcttcag cccgacgaac tgtttcgccg ggctgatttt tcaggattcc
    14281 tgacgtaaat aaatagcccg ataacaaata ctcaccagca ctgcaccgcc gataatatta
    14341 cccagcatca ccgggagcag attggcggta ataaaatggc tgaccgtcag tgcaggaaaa
    14401 ttgtctgcgc tactgtgcgc cagttgccag aaggaggtag gggcgaaatg gcgaatggca
    14461 atggcgaacg gaatcacaaa caaattggcg atacagtgct caaagccact ggcgacaaac
    14521 agggtgatgg gcaaaatcat ggcgacgatt ttgtcgcata acgaacgccc gcaataactc
    14581 atccacagcg ccaggcaaac catcagattg cacataatgc cgaggctgac agattcagta
    14641 aatgtatgat gcattttgcc ctcggcgcag tgtaaaaccg cgactcccca catcgcgttt
    14701 tcactcatca ccagcccgga aaaccagatt aacaaactga aacaggcaat acctgccaga
    14761 ttaccgcagg ccaccagaag tgcgttaatc agccaagttc gccaactaat aacgccccga
    14821 ctttttgcca tcaccgtcat taccgacgag gtgaacaggc tggtgccgca aaccgccagc
    14881 aaaataaagc cgagtgtaaa gcaaaggccg cccaccagat gggttaatgc ctgtgaagac
    14941 ggggcatcgg cgataacgga aaggtaaaaa gtaaatccga tcgccataaa cacgccagcg
    15001 gacatcgcca ttaaaaagac cgacacttta ctgcgggcaa ttttatgggc ggcaatccgt
    15061 tcagcgattg ccgcttttct ggcgctcaac tgcaagtcga aagagagttt gtttctcatg
    15121 catactcctg caaaagcaga ttacaacacc tcgcgaaccg agatccccag acgctgcatt
    15181 cgtgacagca gcgtggtgcg cttcatccct aatcgtgtcg ccgcgccacg gggcccggca
    15241 acaatgccat tggtttctcg caatacctga acaatgcgct gacgttcttc ttcgtcattc
    15301 tctggcgtcg tcgggtgcag caactgcgcc attgaactgc gaagcgctga atcttcattt
    15361 agcgtcggca gtaaacggct ttgtcggaca ttaagatgta aattcagact gttaccgcga
    15421 gtcagcagta ccgctcgctc aatcacgttt tccagctcgc gcacgttgcc cggccaatcc
    15481 cacgacatca actggcgtag tgcctcggtc gggatggcgt caattgcgcg attcatatgg
    15541 cgcgccattt tttgcgtgaa atgttttgct aaaagaggga tatcttccgg acggtctcgc
    15601 agcggcggca attccagtgg gaagacattc aggcgataaa agagatcgct gcgaaactgg
    15661 cgatcttcaa ccatttgcca caaatcacgg ttggtggcgg caatgactct gacattcacc
    15721 gggatcgttc tactcccgcc gagacgctca atttcccgct cctgcaatac gcgcagcaat
    15781 ttaggctgaa gttctaacgg cagatcgcca atttcatcga gaaacaacgt accgccatcg
    15841 gcaatttcaa aacgaccacg atgggtatta atcgcaccag taaaggcccc tttgtcatga
    15901 ccgaataact cactttccag aagactggcg gggatggcag cgcagttgat tttgaccagc
    15961 ggcttgtcgc gtcgcgggct aagttgatgg atcgctctgg cgatcacctc tttgccggtt
    16021 ccggtttcac cacaaatcaa caccgtactg tcgctcttcg ccacaatatc tacctgctgg
    16081 agcaggtctt ccattgcctg gctttgatag ataatgtcac cgacgcccag attcgaaagg
    16141 agctgctcgc taagctggtg gttttcttgc tgcaaacttt cctgcaaatc ggtcatgcta
    16201 cgccaggcat cggcattgcc aacggcaata gcgatgcgat cggctatgtg ttgtagtagc
    16261 tggcagtttt cctcactaaa gagagtggta gaggtatgcg ccagcaacaa tgcacccggt
    16321 gtatggttgc caaaggtaag cggtatcagg agcgaagatt cgcagccgtt agatgcaagt
    16381 aagtgtaata acgggtcgcg ttgccagaga aacagatcgt ctgcacggtg cgtcaaggtc
    16441 ggttgtcggg tttgtagcgt ttgtgtcagc aatacactgt cgccaggcat attgcgtggc
    16501 agacacgcac aatgtgaggc agaaagatcg ctgcaccaca ggctgaactt ctcgttcttt
    16561 cgatgatcgc ccagtaccat actgactgaa gccagaccga aaaaatgatg gatctcacga
    16621 gcgacgtcag cgatcagatc gtcgagatca agatgtgaca gcactgcatt agtgatatcc
    16681 accagtaccc gatgatgatc gcgatctttc tccagcaaac gcagcgccac ctgttgttct
    16741 tcatcattga gccatgattg cagaaccggg gtgataatgc gtgccagcca gttaaacagt
    16801 gtgcaatcct ggggagtaaa acgactgccg tgggtgcgaa taaagcgtag ctccgccatt
    16861 ccactactgc cgagaaggac ggcgtgacgc gaataatcga ccatctcgcc ctgatactct
    16921 gttgttgccg tcacatcgct gccatcatca tggtagcggt aatagaagtt gcttttgaga
    16981 acatgcagtt caataagttg taccgggctg aacggcggcg gcagcgggat gaaggcgcgt
    17041 aataaagacg ccttgccgtg tttctgcgct aaccgctctg cgagcattcc gtttaccgct
    17101 tcaattgtta ttccttgcgg cggggcaaac atcgcctcgt ctgacatagc catcaacagt
    17161 ccttattaat gagaaatcct tctcacgcaa tttttctggc catcagatag atagccggtt
    17221 ccagcacaat ttcatccaac agatgaatga attgaatgct acgcgcttgc cacacagacg
    17281 tcgcgtcatc tgccagtggc gcgaatgccc gggtcaacag ggcggtgtgg ctggcgtcaa
    17341 taacaatctc accaaaagtc atcaccccca gcatcttacg tcgggcttgc tcctcttcga
    17401 ccagtgcaag ccaatcttcg tattccgtca gtgggcagcg gaaggtgaca ttcagacaat
    17461 caatcacgcc aacgtgatga ccgatagcca gcgagtaata cattacctgg gagctgtgtt
    17521 ccggcatctc gtcgctactg gcgacaaact tttttcgcag cgtccagaaa acaatttccc
    17581 cgcactcttc agtcatgtaa cccccggagt acgcgaaaga gttgctgaac gatactattg
    17641 agacgtggat cgtcggcatc gcgcaaccag gtgttaaccc gatttcctgt aggatcgttg
    17701 cttaaatggc gcaggagccg atcgcaaatt tcttgcccct gacgatagcc cgccagccgc
    17761 cgcgcttctc gctcaatggc gatacgctgt gatggcggga tctgcggcca caacggttgc
    17821 atagtcaccc cggtgggatc gcgataatcc accgcgtgaa tcttctgttg cagcaaaccg
    17881 agcgccacgg cgaaaccgtg aatggtggcg gccggtgttg gcgggcagcc ggggatccaa
    17941 acatcaatgg ggacaatggt gtcgctgccg ccccagacgc tgtagagatc gtggaaaata
    18001 ccgccgccga caccgcacgc gccgtaggaa acacagattt tatgatcggg ggcagactca
    18061 tacgcccgaa gtgcaggcat acgcatcgcc cgggtgactg cgccagtaaa taacaaaata
    18121 tcggcgtgac gcggtgatga aacaacctta atgccaaaac gttctgcgtc gaatacgggt
    18181 gtgatggcgg caaaaatttc gatttcacag gcgttgcagc cgccgcaatc gacgcgataa
    18241 acgtaggctg agcgacggat atcctgtagc aaatgccgct tcatcttttg cgtttgctcg
    18301 tccagcgtga tgggctggct gacatgttgt gtaagcactg gactcatagc tgctccttag
    18361 ccaccagcgg tacatctgta tcgtcgttga tcagcgtcgc gcgttgtttg cactccgggc
    18421 agacgctcgc ttgcgcccgc aacatttcgc ggttttgtgg cgcattttgt tgctgtgcca
    18481 acaattcagc agccagtgcg acggtttttt gcggggcaaa cgggcgttcg caacggctgc
    18541 aacgttgtag atggaacgtc gcgcgggtat agagatcggc tttattggtg acggtcagtt
    18601 caaagttatt ggtaagctgg atggctctgg tcgggcacac ttcttcacaa cgtccgcagt
    18661 aaatacaacg ccccagatag agctgccagg tgcgcgaatt ttgctggtcg tcggtctgga
    18721 tagtcagcgc atttgtcgga caagcacagg cgcaggcacc acaggcaata cattggctgg
    18781 gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg aagggatatt
    18841 tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc ttctgtcctt
    18901 acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta tacggcacgg
    18961 tttttgactg gcgcttgcgc acatcaacca gcgtcacacg gtcggtacag gagtagcagg
    19021 gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc agcatgtaac
    19081 gcaacaccgg ccagttggcg taggtggcgg cacggcagcg ccagcggaac aatttttggt
    19141 tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca aagccgagcg
    19201 cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc ggcatgttgt
    19261 cgagagcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg accatcacgc
    19321 gggagaaaac atcgccgccg gtgaaggtaa acagtgtttt tggaatattg ccgtagtcgg
    19381 cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc agcggcccta
    19441 cagggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt
    19501 ccatattcgg cgtggcaagc agcatttcta ccagctccga cacgtcggcg cgcatctcgc
    19561 gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga acaccaccaa
    19621 tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatt gtcatcgact
    19681 tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa tccaacaaaa tggcaggaga
    19741 ggccaaggtt gagcaaatga ctgtgtagcc gttcgacttc cagcagaatc gagcgaatgg
    19801 tatgcgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg gtataggcca
    19861 cactgtgggc aaaaccgcaa atcccacaca cgcggtccga taagaaggtc acttcgttgt
    19921 agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac agacggtaat
    19981 cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccggt tcatcggagg
    20041 tgatatgcag cgggccgaca gggatcaccc gcgcaccgct gttgccctca ttgataaacg
    20101 gatacgtttc ggaatcagtc gtcggttcag ggcgcagtcg ataatccatc gcatctttgc
    20161 gcagcggatg catatcttcc ggccagtcat cgggcaacac caggcgacgc tgatccggca
    20221 ggccaaccgg aatcagcccg tacatatcac gaatttctcg ctcgccccag accgcggcag
    20281 ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagc gccttcacca
    20341 caatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg taatgaccgt
    20401 taagtgtccg ctcgtcgttg ccaaacagga ccggaagcca gccatcatgt tgataataaa
    20461 gatactgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc tgttcaggcg
    20521 tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg
    20581 cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac agatgcggtg
    20641 cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag
    20701 aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat atgcgttccc
    20761 atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca gccgagatca
    20821 ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgcagccat ccgtaccagg
    20881 cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag gtgattacgt
    20941 gccagtccgg cggtaacggt cataaattcg ctaagaaaaa tgttgaaggg cggcatccct
    21001 gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa atggcatgat tttgagcatc
    21061 ccacagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc ggaaccgcag
    21121 aacagcagcg tttttgccag actgtggttt aaggtgtgca gcagcgcggc aaaaattccc
    21181 agcggcccgc caatgcctag cgccaccgcg accagtccca tgttctccac gctggagtac
    21241 gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac ggcaaccgac
    21301 aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc ctggcaaata
    21361 atgatgtaat agcgaatcag caccaacagc gcgcagttca gcaatacggc ggagagtagg
    21421 gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg catgggaaat
    21481 agcccggttt tggtaccaaa gccaattagc acaaacacaa aggccaacag cattaatgtt
    21541 ggatcaagca aggacgattg cttaagaacc tcgctccaga atatcgccat ttctgcctgc
    21601 ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc aaaagcgaca
    21661 ccaacagtac aaataatgat gtacttccat gctgcttcca gcgatgaacg ctgaccgtaa
    21721 atgcctacca gaaacgccga gcttaaggtg gtggcttcga tcgccgccca catcacaatc
    21781 aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa gaaaccgtag
    21841 taatcgcaca gcgtaacggg tgaaagctcg ccgtgctcaa cttcatgacg catgtagcca
    21901 atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa caaaccgccc
    21961 agaccatcaa tatgaagcca cagtcccgca gcgaatattt ctcctgcatc agcggcagtt
    22021 tggaccaccc agagtgccag aatcagcagc agtgtgatcc ctaagctatg taatactgtc
    22081 accgtgcgag tcgcagagtg ccctcgtttc cggcaggcaa aacagagcag cgaaaaaagc
    22141 agcggagtga gcaggagtaa agcgaacatc acagaataac tcatctcatt atcccttcag
    22201 cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc catatttttc ttgccagtaa
    22261 caccatcaca atgacggcga agatggcgtc ggtagcgata ccaatttcca ccagttccgg
    22321 tgcacgccag gctagaagcg ccagcaccag atgggagccg ttttccatca ggcagtaacc
    22381 aaaaatttgc cgcaggatat tgcgctggct aacaatgcac agcaggccaa gcagaaaatg
    22441 acctaacgcc accgccagcg ctggtttcag cccggtagcc atcggtagct tcacgggctg
    22501 aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgccatca ttgccggacc
    22561 gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag tcatgattaa
    22621 cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca gttgctctgc
    22681 ggcgaacagg cacgagaggg tggcgaaaat agacaccagc accagcgact ggcaggcgta
    22741 aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg tcagcatcat
    22801 cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc ttactgcaac
    22861 caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat cagcaccagg
    22921 cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt gccggggacg
    22981 acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat catcagcaga
    23041 atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccggc agcaaacagc
    23101 gggaatttac tgaagaagcc gttgaacggc ggtacgccgg taatcgccag cgctgccacg
    23161 cagaaaccca cgcctggcaa cggcagggtg tgcaatacgc cacgcagacg cggcaacaag
    23221 cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct tttagcgaac
    23281 gcgtggttga cgatgtaggc gatgctgccc tccagcgcca gccgcgagcc gaagatggag
    23341 agcgacaagc cgaagaacat ccagccaagt tgagtgatgg tcgaccaggc cagcaaccgc
    23401 ttcatatcct gctgtggcaa atacatcaga aatccataaa gaatggtgac cagggccatg
    23461 accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat ggcgcgggca
    23521 aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga gataggcgct gatcggtgtt
    23581 ggcgcttcca ttgcgtccgg tagccacgct tgcatcggta gctgggccga tttcccccac
    23641 gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccgtgtaa ctcgctcatc
    23701 gcgctaagcg caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag atacaacccc
    23761 aacgaaccga tatgggtgat aagcaacgct tttagtgctg aacgctgcgc tttatcgctc
    23821 tgataataac tgatcaacac ccaggagcag ccgcccgtaa tttcaaaaaa caacaactga
    23881 ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac cagcaaaaat
    23941 gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc
    24001 gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtacac
    24061 acgcgatcaa tgaccaggcc aaacaatgcc acatccccca cgttgaccaa cgtcaacgtg
    24121 acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca cagcgtggtc
    24181 agcgcggcga acaaaacccc ccattcggcg gcccgacgtt gtggcgaaaa cgaaacgacc
    24241 agtgcgccga taaaaggcag caataacgtc gtcagagcaa gattttccat attccgtcag
    24301 tgctccttac agaccggtta accagaagac ccatgcaagc gcagcaaggc tgaagccaag
    24361 ccaggtcaca tggtgaatga gtaaaaaacg cccgcgtgcc agcgtgtttt ctgcgattga
    24421 ggccagtaca aaaatcagca aaaccttgag cagcgtaacg acaagtgaag tcagcaggca
    24481 ggcgagagaa agttcttgcg cgcacccaaa gggcagaaac agggccacaa acagtgctgc
    24541 catcacgacc tgtttcagcc ccagccccca tttcgccagc gccagcccgg caccggaata
    24601 ttctgtcagc gggccttcct gtaattcctg ttctgcttca gcaacatcaa agggaatttt
    24661 tcccatctca atgaagcagg caaaaccaca ggccagtaac gccagtacgg tggttagcgg
    24721 cgagttccag cccgttgcca gcgtattgct gatcatctcg atatgcgtgg aacctgctat
    24781 cagcgccaat accagcagtg agagaatgag cattggttcg accagaatgc cgagcgtcaa
    24841 ctcgcgactg gcaccgactc ccgcaaacgg gcttccggta tccagcccgg aaagagcaaa
    24901 gaaaaaacga aacagggcaa gaagatagat aagggtgatc agatcgccgc cgcccgcaaa
    24961 aggggaaacg gtaataaata gtggtagggc catcgccagc accagcatgc tgctgattaa
    25021 tacccacggc atcaggcgga acatcagacc tgaagatata ggcgcaactt cctggcgttt
    25081 aaacagtttg tggatatcgc gataatcctg ccagatcccc ggcccgcggc gggagtgcat
    25141 acgcgcgcgt atctgccggg aaatacccgt aaaaagcggg gttagcatca gcagaatcac
    25201 ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa agagtttgtc tcatggtgat
    25261 ttccttagac ggcaatagcg attagcagca caaccagtgc ggcgaccaca tacaggcaat
    25321 agagacgaaa gtcgccgctt tgcagatgct gtatttcttt ggccagccgt tgggtggcgc
    25381 tcacgatggg gcggatcacc cgctcatccc agaagggttc tgtgctctga gccctggcgg
    25441 tgacgtgcgc aagaccttta tttagcctca gcgtagggtc gagctgttgt cgtagacgaa
    25501 atagcgcaga gaagaccaca cgcagcggct gcatcacgcc attgcctgac ggggccatcg
    25561 cattttccca gccgtagccg catgcccagg catctcctgt gcgacgaaac gctgcgcggc
    25621 gcgaacgaca ccacagccag taaaggccag gcagcaaagg catcgccagt agtaacagca
    25681 acagtaatga gggggtgacc tgtgtatgaa acgtgccggg tacaagtgct attccgctgg
    25741 cgacagtgac gggaggggta ttggtaaacg catgggcaat atgcattatc ttcggtgcca
    25801 gccaactggc actaagcgca attaatacgc agagtgccgc gagcagtagc atggcgacga
    25861 tcatcgtatt tggcacttcc tgtgcctctt cagcgtgtgt actgcgcggc gcaccacaga
    25921 aagtaatacc gtacattttg acgaagcaca ttactgccag cccaccagtg actgccagca
    25981 tcaccatagc aataggaccc gcaagttgta gcgctacggc ttccacacga cttagtgaga
    26041 acagcgactg ccaggtgtac cattcgctga taaaaccatt cagcggagga agggctgata
    26101 tcgcgaggca accaatcagg catgctgcgg ctgtccacgg catccgtttc gccagtgccc
    26161 ccattttttc catgtcgtgg gtatgcaaac gcgaaataat cgcgcccgcg ccgagaaata
    26221 gcagcccttt gaacagcgca tggttgagca gatgaaacag tgcgccgagc agtccaacca
    26281 cggtgagcag cgggtcgtgt agtgacagac cgaccatcgc cacaccgact gccagcaaaa
    26341 taatgccgac gttttcgacg gtactccagg ccagcagccg tttgatatcc tgttccgcca
    26401 gcgcatacag cacgcccagg agcgcggaga ttgcgccgat cgccatcacc agaatgcccc
    26461 accacagcgg caaacccgtt tgcgccagca gatccatcgc cactttcagg atgccgaaaa
    26521 taccgatttt gaccattacg ccagacatca acgccgaagc gtgcgatggt gcggcagggt
    26581 gagcgcgcgg caaccagctg tgcaacggca tcatcccggc tttcgcgcca aaaccgaaaa
    26641 aggccagcag gaacaccgcc gacgccagcc ccggagaaag tgaaagcgtg cggaaactgg
    26701 caaaatctag gctgccgctt tcgcgcccca tcagcaagaa ggcgatcata atcagcaccg
    26761 atccggcgtg ggcgataaaa aagtagagca tgccagcgtt gatcgacgtt ttatcctgcc
    26821 tggcaatgac cagaaaccag gaagacagcg acatcatttc aaacagcacg atgaaccaaa
    26881 aagcgttgtc catcaccagc agggcaacca tcgatgcgat gaaaatattc ataaagaagc
    26941 ccatcgccgc cgcgcctttg ccctcgtatt cgcgcatata agtcaatgaa tagagcgaac
    27001 aaaccaccac cagcaaggag ataaccagca ccataaatgc cgagaggcta tccattcgca
    27061 gggttaaatc ggcaaagtca aaaggggtgg caaaacgggc aactaaagtt acgccttcat
    27121 gtaattgcgt aattccgctg atgatgccaa tcacgccgcc caccagcgat gtgatgccgg
    27181 aaagcttaat agccagtctg tccagaccga gtaaaaacag cgaagccaga ctggcaaaca
    27241 gatagaggat cagcgaccag gttaataatt gcagggcatc cattagcgtt gctcctgagt
    27301 gagggaaagg ggatcctccc tgccattggc aaaccagctt gcggcaaggc gctgtttttc
    27361 tttcatctga cgttgcaggc tatcgtcggt gatcagccgt aacgcctgat tcgggcaagc
    27421 gcgaacgcag gcaggacctt ctggcaagaa atcacacagg tcgcatttca ccgcgacggt
    27481 ctggacaccc ggttcccagc gcagcaaagc atgttgtgtt ggcgcgtttt cttcgccgtc
    27541 tttgagtgag ccttcagcct gaaaaacata ttgcgcaggg gcattcaccg gacggcttcc
    27601 tgaagcgctg attgcgccaa atgggcagac cacggcgcaa agcttgcagc caatacagag
    27661 gctttcgttg agttggatcg catcatccct ctgggagatg gcattgaccg ggcagacctg
    27721 cacgcaaggg gcttcctcac agtgatgaca cacgacaggg gcagtgagtg ttgacgtctt
    27781 cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc
    27841 agcgagacag gtattacatc ctgtacacca cagtggttcg gccaccacaa agcggttcat
    27901 gtcggctcct tgaaaatgat atgaaacatt gcatttcata atgcataaag gatgtcagta
    27961 tgattaattt aatatattca ataagttatg aattaactga attctgaacg gtacaaaaat
    28021 gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc gtgtcgcagg ggaaagtgag
    28081 aatgtgatct tgatcacgat aagtgaggtg tgctgaccgc aagcgcagcc agcacggaat
    28141 ggagcaaagt aatcaggcgt gttctttcag ccagttcagc acaacgtcgt ggtgattgct
    28201 ggttttgaaa tcgtcaaaga catgttcgat tttgccatca gcgtcaatca ggaagctgat
    28261 gcgatgaata ccatcgtagg ttttgcccat gaaggacttt tccccccaga cgccgaattg
    28321 ctcgcacacc tggtggtcct catcagacag gagcgtaaag ttaagcagct ctttttccgc
    28381 aaaacgggag agtttttcgg gtttatcggt gctgataccc agcacatcaa cgcccgcttt
    28441 tttcaactca tccatgttat cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat
    28501 ggctttcggg tagaaataaa ccagaacacg ctgtccctgg aagtcggtca aattaacttg
    28561 ttctccgtct tgatccggca agctaaattt cggtgcgata tcaccggctt tcagtggatt
    28621 cattacttaa ctccatcctg ttcatcatgt tgggaataat tgacgacgtt aatactgcct
    28681 tgtgcattga gttctgtaca tagggctttg aacgcttgct caatatttgc tgcgtccgca
    28741 gatgcggggc tgtgggcagt aatctgaata tgcaactgcg cagcccgttc attttcagca
    28801 ggttgtgtgc gcgacaccag ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg
    28861 aagcgttcaa ttaaatgcgg ggagtctgcc acatcgacct gaacccagac agatgctggc
    28921 attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga ttaaaagatc cagttcggca
    28981 cctttcaacg gtaacgttga ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc
    29041 atgataaacg tgaactcttc tcccagcatc gccaggcgac tgtcttcaat attacagccg
    29101 caactactga catgacgggt gatggtgttc acaattccag ggcgatcggc acccaacgca
    29161 gtgatcacca gataatgttg cgatgacagt gtcaaactgg ttattccttt aaggggtgag
    29221 ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc
    29281 ttgcttttaa tgccatacca aacgtaccat tgagacactt gtttgcacag aggatggccc
    29341 atgttcacgg gaagtattgt cgcgattgtt actccgatgg atgaaaaagg taatgtctgt
    29401 cgggctagct tgaaaaaact gattgattat catgtcgcca gcggtacttc ggcgatcgtt
    29461 tctgttggca ccactggcga gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg
    29521 atgatgacgc tggagctggc tgacgggcgc attccggtga ttgccgggac cggtgctaac
    29581 gctactgcgg aagccattag cctgacgcag cgcttcaatg acagtggtat cgtcggctgc
    29641 ctgacggtaa ccccttacta caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa
    29701 gccatcgctg agcatactga cctgccgcaa attctgtata atgtgccgtc ccgtactggc
    29761 tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc
    29821 aaagaggcaa cagggaactt aacgcgtgta aaccagatca aagagctggt ttcagatgat
    29881 tttgttctgc tgagcggcga tgatgcgagc gcgctggact tcatgcaatt aggcggtcat
    29941 ggggttattt ccgttacggc taacgtcgca gcgcgtgata tggcccagat gtgcaaactg
    30001 gcagcagaag ggcattttgc cgaggcacgc gttattaatc agcgtctgat gccattacac
    30061 aacaaactat ttgtcgaacc caatccaatc ccggtgaaat gggcatgtaa ggaactgggt
    30121 cttgtggcga ccgatacgct gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag
    30181 acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa gtttagggag atttgatggc
    30241 ttactctgtt cataagtcgc gcctggcaac ggttgcgggt gtttcgcttg ttttattact
    30301 cgctgcctgt agttctgact cacgctataa gcgtcaggtc agtggtgatg aagcctacct
    30361 ggaagcggca ccgcttgcgg agcttcatgc cccggctgga atgattttgc cggtgacctc
    30421 cggtgattat gcaatcccgg tgaccaacgg tagtggtgct gtcggtaagg cgctggacat
    30481 tcgtccacca gcccagccgc tggcactggt ttctggcgcg cgtacccagt tcacgggcga
    30541 taccgcttca ttgctggtgg aaaatggtcg tggcaatact ctgtggccgc aggtggttag
    30601 cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat gatgctggtc agacactgac
    30661 caccgattgg gtacaatgga accgtctgga cgaagacgag cagtatcgtg gtcgttatca
    30721 aatctctgtt aagccgcagg gttatcagca ggcggttacg gttaaactgc tgaacctgga
    30781 acaggcgggc aaaccggttg cagacgcggc ttccatgcag cgttacagca cggagatgat
    30841 gaacgttatt tccgctggtc tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa
    30901 tcgtgcctcc accactatgg acgtacaaag tgcagctgat gacaccggtt taccaatgct
    30961 ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt
    31021 gggcatgaaa gtgaccgaca gcacccgttc gcagggcaac atggccgtaa cttataagcc
    31081 gctgtctgac agcgactggc aggaactggg cgcgagcgat ccaggcctgg catctggtga
    31141 ctataaactg caggttggcg atttagataa ccgcagcagc ctacagttca tcgatccgaa
    31201 aggtcatact ctgactcaga gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt
    31261 tagcaagtaa aaatacaggg ctggaatcat ccggcccttt tttctgatat gatacgcaaa
    31321 cgtgtgcgtc ggcaggaaaa cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg
    31381 acgtagttcg gataaggcgt tcacgccgca tccgacaaaa catccggcac accagacagc
    31441 aaaagatttt aaaacgttaa ttcacaccca ggagtgataa agatgcaaaa gcaagctgag
    31501 ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa acccggacct gttggtgctc
    31561 gaattccgca atgatacgtc agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa
    31621 ggtatggtga acaacaagtt caactacttc attatgagca aactggctga agcgggtatc
    31681 ccgactcaaa tggagcgtct gctctccgat accgaatgtc tggtgaaaaa gctggatatg
    31741 gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga
    31801 atcgaagaag gtattgagct gaacccgccg ctgttcgatc tgttcctgaa aaacgacgcc
    31861 atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct ttggctgggt aagcaaagag
    31921 aacctggcac gcatgaaaga gctgacctac aaagcgaacg acgtgctgaa aaaactgttc
    31981 gatgatgctg gtctgattct ggtcgacttc aagctggagt ttggtctgta caaaggtgaa
    32041 gtggtactgg gcgatgagtt ctccccggac ggtagccgcc tgtgggacaa agaaacgctg
    32101 gagaaaatgg acaaagaccg tttccgccag agcctcggtg gcctgatcga ggcctatgaa
    32161 gccgtcgccc gccgcctggg tgtacagctg gactgatttt tctgttcatc atcttgccgt
    32221 gctgctggca cggcaagaca accattctcg taagatgtgc attgaacgct cattcctccg
    32281 ccatttcatc ccgttttcct gtggtaatct tcctctgaac cacctacgat catttctata
    32341 atgaatatat tgttgaggta tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg
    32401 aagacaggcg caatagctcc ggcggtccgt caatgggcgg tcccggtttc cgtctgccga
    32461 gcggtaaagg cgggctgatt ttactgatag tcgtgctggt tgcaggctac tatggcgttg
    32521 atttaaccgg gttgatgacc gggcagccgg tttctcaaca gcaatcaacg cggtcaatta
    32581 gcccaaatga agacgaagcc gcaaaattca cctcggtgat tctggcaacc acggaagaca
    32641 cctggggaca acagttcgag aagatgggta agacctatca gcaaccgaag ctggtcatgt
    32701 accgtggaat gacgcgtacc ggatgcgggg cgggccagtc cataatgggg ccgttctatt
    32761 gcccggcgga tggtacggtt tatatcgatc tctccttcta tgatgacatg aaagacaaac
    32821 ttggcgcgga tggcgatttt gcccaggggt acgttatcgc ccatgaagtc ggtcatcatg
    32881 tgcagaaact gttaggcatc gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg
    32941 cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc cgactgtttt gccggtgtct
    33001 gggggcatag catgcagcag caaggcgttc tggaaaccgg cgatctggaa gaggcgctga
    33061 acgcggcgca ggccatcggc gatgaccgtt tacaacagca aagtcagggg cgagtagtac
    33121 cagacagttt cactcatggc acttctcagc aacgctacag ctggtttaaa cgtggtttcg
    33181 acagcggcga tccggcacaa tgcaatactt ttggtaaaag catttaactt ccggggcagg
    33241 gatggctgaa ctgactgcgc ttcacacatt aacagcgcaa atgaaacgtg aagggatccg
    33301 ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt gatcatgcgc ttaagttacg
    33361 tgatgcctta cctggcgact ggctgtggat ttcgccgcag ccagatgctg aaaaccactg
    33421 ttctccctcg gcactacaaa ctttacttgg gcgcgagttc cggcatgcgg tattcgacgc
    33481 ccgccacggc tttgatgccg ctgcctttgc cgcacttagc ggaacgttga aagcgggaag
    33541 ctggctggtt ttgttactcc ctgtatggga agagtgggaa aaccaacctg atgccgactc
    33601 gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg cattttgtcc agcatttcaa
    33661 acgcgtactt acggcggata acgacgctat cttctggcgg caaaaccagc cgttctcgtt
    33721 ggcgcatttt actccccgta ctgactggca ccccgcgacc ggcgcaccac aaccagaaca
    33781 acagcaaatc ttacagcagc tactgaccat gccgtcgggc gtggcagcgg taactgctgc
    33841 gcgtgggcgc ggtaaatcgg cgctggcagg gcaactcatt tctcgtattg cgggtagtgc
    33901 gattatcact gcgcccgcaa aagcggcaac ggatgtactg gcacaatttg cgggcgagaa
    33961 gtttcgcttt attgcgcctg atgccttgtt agccagcgat gagcaagccg actggctggt
    34021 ggtcgatgaa gccgcagcca tacctgcgcc gttgttgcat caactggtat cgcgttttcc
    34081 tcgaacgttg ttaaccacta cggtgcaggg ctacgaaggt accggacgtg gttttttgct
    34141 gaaattttgc gctcgctttc cgcatttaca ccgttttgaa ctgcaacagc cgatccgctg
    34201 ggcacaggga tgcccgctgg aaaaaatggt tagtgaggca ctggttttta acgatgaaaa
    34261 cttcacccat acaccacaag gcaatatcgt catttccgca tttgaacaaa cgttatggcg
    34321 aagcgagcca gaaacgccgt taaaggttta tcagttattg tctggtgcgc actaccggac
    34381 ttcgccgctg gatttacgcc gcatgatgga tgcaccaggg caacattttt tacaggcggc
    34441 tggcgaaaac gagattgccg gagcgctgtg gctggtggat gaggggggat tatctcaaga
    34501 actcagtcag gcggtatggg caggttttcg tcgcccgcgg ggtaatctgg tggcccagtc
    34561 gctggcggcg cacggcagca atccactggc ggcgacattg cgtggacggc gggtcagccg
    34621 gatagcagtt catccggcgc gtcagcgcga aggcgttggg caacagctca ttgccagcgc
    34681 tttgcaatat aggcctggcc tcgactatct ttcggtgagt tttggttaca ccggggagtt
    34741 atggcgtttc tggcaacgct gcggttttgt gctggtgcga atgggtaatc atcgtgaagc
    34801 cagcagcggt tgctatacgg cgatggcgct gttaccgatg agtgatgcgg gtaaacagct
    34861 ggctgaacgt gagcattacc gtttacgtcg cgatgcgcaa gctctcgcgc agtggaatgg
    34921 cgaaacactc cctgttgatc cactaaacaa tgccgtcctt tctgacgacg actggcttga
    34981 actggccggt tttgctttcg ctcatcgtcc gctattaaca tcgttaggtt gcttattgcg
    35041 tctgctacaa accagtgaac tggcattacc ggcgctgcgt gggcgtttac agaaaaacgt
    35101 cagcgacgcg cagttatgta ccacacttaa actttcaggc cgcaagatgt tactggtccg
    35161 tcagcgggaa gaggccgcac aggcgctgtt cgcacttaat gatgttcgca ctgagcgtct
    35221 gcgcgatcgc ataacgcaat ggcaattttt tcactgactc cttcaatttt ctcccatggt
    35281 cattgtgagt cagcggcgta aaattaatga catcagttaa ggagttcgcc atgaaacatg
    35341 accattttgt tgttcaaagc ccggataaac ctgcgcaaca gttgttactg cttttccacg
    35401 gtgtcgggga taacccggtg gcaatggggg aaataggtag ctggtttgca ccgttgttcc
    35461 cggatgcgct ggtggtgagt gtcggtggcg cggagccgag cggtaatccg gcggggcgtc
    35521 agtggttttc ggtgcagggt attacggaag ataatcgcca ggcgcgtgtg gatgccatca
    35581 tgccgacgtt tattgaaacg gtgcgctact ggcagaaaca gagcggagta ggggctaacg
    35641 ccacggcgct aatcggtttc tctcaaggcg cgattatggc gctggagagc attaaagccg
    35701 aaccgggcct tgcttcacgc gtcattgctt ttaacggacg ctatgcgagc ctgccggaaa
    35761 ccgcgtctac agccacgaca attcacttga ttcatggcgg tgaagatccg gttatcgatc
    35821 tggcgcatgc agttgcggcg caggaagcgt taatcagtgc cggtggtgac gtgacgctgg
    35881 atatcgtgga ggatttaggc catgcgatcg acaatcgcag tatgcaattt gccctggatc
    35941 acttgcgtta taccattccg aaacattatt tcgacgaagc gctaagcggc ggtaagcctg
    36001 gcgatgatga tgtgattgaa atgatgtaat aatagccggg taggcctgat aagaccgtat
    36061 caggcatcat ggccggatgc ggcgttaacg ccttatccgg ccacttagct tacttcttcg
    36121 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
    36181 ggggaagctc cggcttgttg gcgagaaatt tcttatggtc gacgcgcttg agatctttaa
    36241 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
    36301 gccagtccat gcttatttcc tcttgcagaa ccactcatca cgcgacgagc tgttccatga
    36361 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
    36421 tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg cgggcaataa
    36481 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
    36541 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
    36601 aaagccacca atccaccgta tagcgcagtt gatgtttttc aagcagggca agcacctgcg
    36661 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
    36721 cacccggaat aacgttgtta ctgcccgttc ccgcctgaat attggcaatc tgcatactgg
    36781 tcgccgggaa gaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
    36841 gtgctgcgcg atgtaccgga ttgtcagcca gatgtgggta ggcaacatgc ccctgaacgc
    36901 catgaatggt taggttgcag gttaacgatc cacgacgacc atttttcacc acatcaccta
    36961 ccacttcgat actcgacggt tcgccgacca ggcagtaatc gagacgctca ttacgtgcca
    37021 ttaacgcttc gacggctttt accgtaccat tgtgggcact ggcttcttca tcagaggtga
    37081 tcagaaatgc cagtcgccct gtatggttgg gatgttgtgc gacaaaacgt tctgcagcta
    37141 ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccgt
    37201 cacgaatggt tggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
    37261 cgtcggtatg cccggcaaag gccaatgttt caccttgccc acgccatgcc caaaaattct
    37321 gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca
    37381 acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
    37441 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaatttc tgataactgg
    37501 aatcactgaa acccagcagc ataggcttac caggcgcgca gagcaatgga cgtttgataa
    37561 ttgcaggcat ttcagtcatt aatgccgccg cagaggccgc atcggtgatt atattgcggg
    37621 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt gagtaacgct tcccagccta
    37681 attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    37741 aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg gtgtcacaat
    37801 ttttgatgcc gtaaa
//
LOCUS       DAZFKY010000024        37384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFKY010000024 DAZFKY010000000
VERSION     DAZFKY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..282
                     /gene="ytfI"
                     /locus_tag="ACHKCL_001099"
     CDS             <1..282
                     /gene="ytfI"
                     /locus_tag="ACHKCL_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO5762114.1"
                     /translation="ALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQ
                     VDAIVSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(345..899)
                     /gene="ytfJ"
                     /locus_tag="ACHKCL_001100"
     CDS             complement(345..899)
                     /gene="ytfJ"
                     /locus_tag="ACHKCL_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5762115.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1224..1430
                     /gene="ytfK"
                     /locus_tag="ACHKCL_001101"
     CDS             1224..1430
                     /gene="ytfK"
                     /locus_tag="ACHKCL_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5762116.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1526..2869)
                     /gene="paeA"
                     /locus_tag="ACHKCL_001102"
     CDS             complement(1526..2869)
                     /gene="paeA"
                     /locus_tag="ACHKCL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5762117.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3192..3830)
                     /gene="msrA"
                     /locus_tag="ACHKCL_001103"
     CDS             complement(3192..3830)
                     /gene="msrA"
                     /locus_tag="ACHKCL_001103"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5762118.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4036..5769
                     /gene="tamA"
                     /locus_tag="ACHKCL_001104"
     CDS             4036..5769
                     /gene="tamA"
                     /locus_tag="ACHKCL_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5762119.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5766..9545
                     /gene="tamB"
                     /locus_tag="ACHKCL_001105"
     CDS             5766..9545
                     /gene="tamB"
                     /locus_tag="ACHKCL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5762120.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9548..9889
                     /gene="ytfP"
                     /locus_tag="ACHKCL_001106"
     CDS             9548..9889
                     /gene="ytfP"
                     /locus_tag="ACHKCL_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5762121.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10101..10352
                     /gene="chpS"
                     /locus_tag="ACHKCL_001107"
     CDS             10101..10352
                     /gene="chpS"
                     /locus_tag="ACHKCL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO5762122.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10346..10696
                     /gene="chpB"
                     /locus_tag="ACHKCL_001108"
     CDS             10346..10696
                     /gene="chpB"
                     /locus_tag="ACHKCL_001108"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO5762123.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10776..11306)
                     /gene="ppa"
                     /locus_tag="ACHKCL_001109"
     CDS             complement(10776..11306)
                     /gene="ppa"
                     /locus_tag="ACHKCL_001109"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5762124.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11616..12572
                     /gene="ytfQ"
                     /locus_tag="ACHKCL_001110"
     CDS             11616..12572
                     /gene="ytfQ"
                     /locus_tag="ACHKCL_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5762125.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12712..14214
                     /gene="ytfR"
                     /locus_tag="ACHKCL_001111"
     CDS             12712..14214
                     /gene="ytfR"
                     /locus_tag="ACHKCL_001111"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5762126.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQARRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVLIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14228..15250
                     /gene="ytfT"
                     /locus_tag="ACHKCL_001112"
     CDS             14228..15250
                     /gene="ytfT"
                     /locus_tag="ACHKCL_001112"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5762127.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15237..16232
                     /gene="yjfF"
                     /locus_tag="ACHKCL_001113"
     CDS             15237..16232
                     /gene="yjfF"
                     /locus_tag="ACHKCL_001113"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5762128.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16265..17263)
                     /gene="fbp"
                     /locus_tag="ACHKCL_001114"
     CDS             complement(16265..17263)
                     /gene="fbp"
                     /locus_tag="ACHKCL_001114"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5762129.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17439..18812
                     /gene="mpl"
                     /locus_tag="ACHKCL_001115"
     CDS             17439..18812
                     /gene="mpl"
                     /locus_tag="ACHKCL_001115"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5762130.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(18968..19519)
                     /gene="yjgA"
                     /locus_tag="ACHKCL_001116"
     CDS             complement(18968..19519)
                     /gene="yjgA"
                     /locus_tag="ACHKCL_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5762131.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19613..20965
                     /gene="pmbA"
                     /locus_tag="ACHKCL_001117"
     CDS             19613..20965
                     /gene="pmbA"
                     /locus_tag="ACHKCL_001117"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5762132.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21149..21535
                     /gene="cybC"
                     /locus_tag="ACHKCL_001118"
     CDS             21149..21535
                     /gene="cybC"
                     /locus_tag="ACHKCL_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5762133.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(21580..22044)
                     /gene="nrdG"
                     /locus_tag="ACHKCL_001119"
     CDS             complement(21580..22044)
                     /gene="nrdG"
                     /locus_tag="ACHKCL_001119"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5762134.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22234..24372)
                     /gene="nrdD"
                     /locus_tag="ACHKCL_001120"
     CDS             complement(22234..24372)
                     /gene="nrdD"
                     /locus_tag="ACHKCL_001120"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5762135.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24766..26421)
                     /gene="treC"
                     /locus_tag="ACHKCL_001121"
     CDS             complement(24766..26421)
                     /gene="treC"
                     /locus_tag="ACHKCL_001121"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5762136.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26471..27892)
                     /gene="treB"
                     /locus_tag="ACHKCL_001122"
     CDS             complement(26471..27892)
                     /gene="treB"
                     /locus_tag="ACHKCL_001122"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5762137.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28011..28958)
                     /gene="treR"
                     /locus_tag="ACHKCL_001123"
     CDS             complement(28011..28958)
                     /gene="treR"
                     /locus_tag="ACHKCL_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5762138.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTERSEPQQIIIPANLS"
     gene            29143..29196
                     /gene="mgtL"
                     /locus_tag="ACHKCL_001124"
     CDS             29143..29196
                     /gene="mgtL"
                     /locus_tag="ACHKCL_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5762139.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29337..32033
                     /gene="mgtA"
                     /locus_tag="ACHKCL_001125"
     CDS             29337..32033
                     /gene="mgtA"
                     /locus_tag="ACHKCL_001125"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5762140.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32239..32625)
                     /gene="ridA"
                     /locus_tag="ACHKCL_001126"
     CDS             complement(32239..32625)
                     /gene="ridA"
                     /locus_tag="ACHKCL_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5762141.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32698..33159)
                     /gene="pyrI"
                     /locus_tag="ACHKCL_001127"
     CDS             complement(32698..33159)
                     /gene="pyrI"
                     /locus_tag="ACHKCL_001127"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5762142.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33172..34107)
                     /gene="pyrB"
                     /locus_tag="ACHKCL_001128"
     CDS             complement(33172..34107)
                     /gene="pyrB"
                     /locus_tag="ACHKCL_001128"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5762143.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34111..34245)
                     /gene="pyrL"
                     /locus_tag="ACHKCL_001129"
     CDS             complement(34111..34245)
                     /gene="pyrL"
                     /locus_tag="ACHKCL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5762144.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34526..34921)
                     /gene="yjgH"
                     /locus_tag="ACHKCL_001130"
     CDS             complement(34526..34921)
                     /gene="yjgH"
                     /locus_tag="ACHKCL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5762145.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35052..35765)
                     /gene="bdcA"
                     /locus_tag="ACHKCL_001131"
     CDS             complement(35052..35765)
                     /gene="bdcA"
                     /locus_tag="ACHKCL_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5762146.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            35836..36429
                     /gene="bdcR"
                     /locus_tag="ACHKCL_001132"
     CDS             35836..36429
                     /gene="bdcR"
                     /locus_tag="ACHKCL_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5762147.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            36574..37026
                     /gene="tabA"
                     /locus_tag="ACHKCL_001133"
     CDS             36574..37026
                     /gene="tabA"
                     /locus_tag="ACHKCL_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5762148.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37149..>37384
                     /locus_tag="ACHKCL_001134"
     CDS             37149..>37384
                     /locus_tag="ACHKCL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762149.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFLEFLVKIFTLGYVDFSKRSNEAG"
BASE COUNT         8988 a         9311 c        10055 g         9030 t
ORIGIN      
        1 gcgcttaaaa tctatggagg ggatgaacgt tttcgtaatg gttcctctgt tgtcttggag
       61 gatgaagatt actctcttat ttataatttt ctggttaatg cgggttgcca acaagtcgaa
      121 ttagccgaac aagtggatgc gatagtaagt gcgaatctgg ccgcagatag tgatattacc
      181 aaagaaaaga tttgcgagaa atataaatcg accattgagg cgtttaaaaa agagcaacta
      241 gcgttaccag tattagttcg gcgtaagaac tcagagactt aattccccta tcccatagat
      301 aacgataggg gaaaattatt tttgcagaat ttatgttatt gccattattt gagcaacttt
      361 tgcagcaggt ccatcacttg ctgcacctct tcctgagtga gcgccccgtc tttggcccat
      421 tgcacgcgac cgtctttatc cagcaccact acagcggaac tttcttcatc cagctgccag
      481 gcaccgcgcg cgacgccatt gctatcgaca ataaactgcg accagggata aagcttttta
      541 ttactctcca gactgctgcg cacaaacatg ccggaacccg gaatcgcgtc gtcggtgtta
      601 acaatggtgg tggtctggta acgatcgtgc ggtaacttcg ctgatttaat cgcttcaatc
      661 agcgtcgcgt ttttctcttt tgcagaggtg cgaccagcaa tatgttgcag tactcgcact
      721 tttcccacta actgcgcgct gttccaggtt ttgtagctaa actgatcttt atcaagcacc
      781 aactcgcccc gatcggtaat gccaatcggc ggcactcgct gaccggtttc gaactgatgt
      841 gcggaagcca tcatcggcaa cagcaggcag gtgagtgcca gaatcttgcg tagggtcatg
      901 gtgtttcctt cttatgatat gcaggtgatc cgaccacttg ggtctgagtt ttaatcatat
      961 gtgctattta tcgaatttcc cgcaagtgtg atgccagttt gcggtcaagc gcacaaatca
     1021 tatgaaaaat gaatgcttat actgaagacc gcgcttcggt aaaaagataa ttctgaataa
     1081 ttgtaacctt taggtaaaaa aagttatacg cggtggaaac attgcccgga tagtctatag
     1141 tcactaagca ttaaaatttg cgcctcataa tagttgggcc gattgtggca ccgcacaggc
     1201 gtaatactca gcaggagata acaatgaaaa ttttccaacg gtacaaccca cttcaggtgg
     1261 cgaagtacgt aaagatcctg ttccgtggac gattatacat caaggacgtt ggcgcttttg
     1321 aattcgataa gggtaagatt cttatcccaa aagtgaagga taagctgcat ttgtcagtga
     1381 tgtccgaagt taaccgtcag gttatgcgtc tgcaaacaga gatggcttaa ccaaagtgct
     1441 atgcagtaaa aagtgctatg cagtaaaaag tgctatgcag taataagacg gctcctgatt
     1501 caggagccgt tgatgtttat gggggttacg cgacgctttc ttctttatct ttagcgtcag
     1561 gcagttttgg cgaaagggct gtggccttgc tgtcgatccg ggtcaccagc agctggtcga
     1621 tgcggtagtt atcgatatcc accacttcaa atttgtagcc ggcgaatttc accgaatcgg
     1681 tgcgtttcgg gatcttacgc agcataaaca tcataaagcc gccgatggtt tcgtagttgc
     1741 ccgactgcgg gaactcgtca atatccagca cgcgcatgac gtcgtcaatc ggagtgccgc
     1801 cgtcaatcag ccatgagttc tcatcacggg cgacaatctg ctcttccagt ccctgaccga
     1861 ccagatcgcc catcagcgtg gtcatcacgt cgttgagggt gatgatcccc actaccagcg
     1921 cgtactcgtt catgatcacc gcgaagtctt ctcctgcggt tttaaaactt tccaacgcct
     1981 ctgaaagggt taacgtatcc ggcacaatca gcgtgttgcg aatttgtacg ccgctgttca
     2041 gtgccaggct ttggttagcc agcacgcggt tcagcaggtc tttagaatcg acatagccga
     2101 tgatgtggtc aatatcttca ttacagacga ggaacttaga gtgcggatgt tccgccacct
     2161 tatttttcag actttgctca tcttcgtgga gatcaaacca aatcacgttt tcacgcggtg
     2221 tcattgaaga cggaacggta cgggattcca gctcaaagac gttttcaatc agctcgtgtt
     2281 cctgtttacg taacacgccc gccagcgcac cggcttccac taccgcgtag atgtcatcag
     2341 aagtgatgtc atctttacgt accattggca gtttgaaaat acggaagatt atgttcgcca
     2401 ggccgttgaa gaaccacacc agcggggtgc aaacgtacag gcagaagcgc atcgggttga
     2461 tgatacgcaa agccacagct tctggcgcaa tcataccgat gcgtttcggg gttaaatccg
     2521 caaacaggat aaacatgcca gtcactaacg agaaagagag aataaagctc agttgctcag
     2581 agagctctgc cgacatatag cgggagaaca ggctgtgaaa agctggagaa aatgccgcat
     2641 caccgacgat accgccgaga atcgccactg cgttcagacc gatttggacc acggtaaaga
     2701 acatgccggg attttcctgc atattcagaa cgcgttgggc atttatattg ccttcatcag
     2761 ccagcagttt aagtttgatt ttgcgtgagg cggcaagtga gatctcagac atcgagaaga
     2821 acgcacttac agcgatcaag cagagtatga ctaaaatact gtttaacata tcttatccga
     2881 cctttcaggc cagatcctcg gaaggggaag ttgattaatt tgtgtgaaat acacattgaa
     2941 agccgattcg aagaaagtga accggcattt tcagtggcta gtatagcgta aggtactgta
     3001 aagccgccag agggttaaaa ttcagacagc tgaaaaatgc aaaactgcct gatgcgctat
     3061 gccgatcagg cctacgcagt ccttgcaata tattgaattt ggacgatttt gtaggcctgg
     3121 caaggcgttt acgccgcatc cggcataaac aaaacgcact ttatcaacaa tctacatttg
     3181 tacccgtaac gctatgcttc cggtggcaga cagacgccaa ttccgccaat tccacagtaa
     3241 ccgtacgggt ttttatgcag atattgctgg tggtcatctt cggcataata aaacggtgtg
     3301 gcgttagcga tttccgtggt gatgtgacga tcgtcatcgg cggcaagcat cgccgcctga
     3361 aaacgttcca gactggcgcg agctgcggca tcctgttctg gggtcagcgg ataaatcgct
     3421 gaacgatact gcgtgccgtg gtcattgccc tgacgcattc cctgggcggg atcgtgattc
     3481 tcccaaaata cctgtagcaa ctgctcatag ctgatgacgg aaggatcgta aacaatgcgt
     3541 accgcttcgg catgaccagt atcaccggag cacacttccc gataagtcgg atttggcgta
     3601 tagccgccgg tataacctgc ggcggtgctg taaacgccgt gtaactgcca gaacagacgc
     3661 tccacacccc agaaacaacc catcgcaaaa atggcaatct ccattccgtc aggtacattg
     3721 gtcattgagt gaccgttaac cgcatgcagc gtggctacgg gcatcggggt gttacgtcca
     3781 ggcagggcat cggcggggga aaccagatgc tttttatcaa ataaactcat ggtgtcgctc
     3841 tcccgaaaat cggtcattgg ggttaaggtt gtaacaagag gcgtatttgc acacaataac
     3901 caccgtgaat aggtctaaag taaaacataa gaaatatttg gggtttagtc tgctttttaa
     3961 tccatattac tggatttttg ttaagccgtt taacggcgtt ccaggggcag gaaaaaagga
     4021 tattcaggag aaaatgtgcg ctatatccga cagttatgct gtgtaagctt actctgctta
     4081 agcggatctg ccgtcgccgc gaacgtccgt ctacaggtcg aggggttatc gggacagctg
     4141 gaaaagaacg ttcgtgcgca gctttctacg attgaaagtg atgaagtgac gccagaccgt
     4201 cgctttcgcg cacgcgtcga tgatgctatc cgcgaaggtc tgaaagcgct gggttattac
     4261 cagccgacca ttgaatttga tctccgtcca ccgccaaaga aagggcggca ggtattgatc
     4321 gccaaagtca cgccaggcgt gccggtgtta attggcggca ccgatgtggt attgcgcggg
     4381 ggcgcgcgga ccgataaaga ctatttgaaa ttgctcgata ctcgcccggc tattggcacg
     4441 gtactgaacc agggcgatta tgaaaatttc aaaaagtcct taaccagcat tgcgttgcgt
     4501 aaaggttatt tcgatagcga atttaccaaa gcgcagctgg gcattgcgct cggcctgcat
     4561 aaagccttct gggatattga ttataacagt ggcgaacgtt accgctttgg gcatgtgacc
     4621 tttgaaggat cacaaatccg cgatgaatac ctgcaaaatc tggtgccgtt taaagagggc
     4681 gatgagtacg aatcgaaaga tctggcagaa ctgaaccgcc gactttctgc taccggctgg
     4741 tttaactcgg tggtggtggc tccacaattt gataaagcgc gcgaaacgaa agtattacca
     4801 ttgacgggcg tggtttcgcc gcgaacagaa aacactatcg aaaccggggt cggttactct
     4861 acggacgtgg gaccgcgcgt gaaagcgacg tggaaaaaac cgtggatgaa ctcatacggt
     4921 cacagtctga ccaccagtac cagtatttcc gcgccggaac agaccctcga cttcagctat
     4981 aaaatgccgc tgctgaagaa tccactggaa caatattatt tggtgcaggg cggttttaag
     5041 cgcactgacc tgaacgatac cgagtctgac tccactacgc tggtggcttc tcgctactgg
     5101 gatctctcca gcggctggca gcgtgccatt aacctgcgct ggagtctcga ccactttact
     5161 cagggtgaaa ttaccaacac cacgatgctg ttttatcctg gggtgatgat tagccgcacg
     5221 cgttctcgtg gtggcctgat gccaacctgg ggcgactcgc aacgctactc tatcgactac
     5281 tccaacacgg cctggggttc agatgtcgat ttctccgttt tccaggcgca gaacgtctgg
     5341 atccgcacac tgtacgatcg ccatcgtttt gtgacacgcg gcacgctggg ctggattgaa
     5401 accggtgatt tcgacaaagt accgccggat ctgcgtttct tcgccggggg cgatcgcagt
     5461 attcgcggct acaaatacaa atctatcgcc ccgaaatacg ctaacggtga cctgaaaggg
     5521 gcctcgaagt tgataaccgg atcgctggaa taccagtaca acgtgaccgg aaaatggtgg
     5581 ggcgcggtgt ttgtcgatag tggcgaagcg gtaagcgata ttcgccgcag cgactttaaa
     5641 accggtaccg gggtcggcgt acgctgggaa tcgccggtcg ggccaatcaa actcgatttt
     5701 gccgtaccgg tcgcggataa agacgaacac gggttacagt tttacatcgg tctggggcca
     5761 gaattatgag tttatggaaa aaaatcagcc tcggcgtggt tatcgttatc ttactgttgc
     5821 tgggatcggt ggcgtttctg gtgggcacca ccagcggcct gcatctggta tttaaagcgg
     5881 cggatcgctg ggtgccagga ctggatattg gcaaggtcac cggcggctgg cgcgatctca
     5941 ccttgtctga cgttcgttat gagcagccag gcgtggcggt aaaagcgggt aatctgcatc
     6001 tggcggtcgg tcttgagtgc ctgtggaaca gcagtgtttg tattaatgac ttggcgctga
     6061 aagacattca ggtcaacatc gacagtaaaa aaatgcctcc ttctgaacag gttgaagaag
     6121 aggaagatag cggtccgctg gatctctcca cgccgtatcc catcaccctg acacgggtgg
     6181 cactggacaa cgtcaacatc aagattgatg acaccacggt atcggtgatg gacttcacct
     6241 ccggcctgaa ctggcaggag aaaaccctga ccctgaaacc gacgtcgctg aaaggcctgc
     6301 tgattgctct gccgaaagtg gcggaagtgg cgcaggaaga agtggtcgaa ccgaaaattg
     6361 aaaatccgca gccggaggaa aagcctctcg gcgaaacact caaagatctc ttttctcgcc
     6421 cggtattgcc ggaaatgacc gacgtgcatc tgccactcaa cctgaacatt gaagagttta
     6481 agggagagca actgcgcgtg acgggcgata cggacattac cgtgcgcacc atgctgctga
     6541 aagtgagcag cattgacggc aatactaaac tggacgccct ggatatcgat tccaatcaag
     6601 ggatcctcaa cgccagcggc acggcgcagc tgtcagacaa ctggccggtg gatatcaccc
     6661 tcaacagcac actgaacgtg gagccgttga aaggtgaaaa ggtgaagctg aaagtgggcg
     6721 gcgcgctgcg cgaacagctg gagattggcg ttaatctttc cggtccggtg gatatggatt
     6781 tacgcgccca gacgcgactg gcggaagccg gattgccgct caacgtggaa gtgaacagca
     6841 aacagattta ctggccgttc actggtgaga agcagtatca ggcggatgat ctgaaactga
     6901 aacttaccgg caaaatgacc gattacacgc tctctatgcg tacggcagtg aagggactgg
     6961 agatcccgcc agccaccatt acccttgatg ccaaaggtaa tgaacagcag gtcaatctcg
     7021 acaaactcac cgtcgcggcg ctggaaggga aaactgaact caaggcgttg cttgactggc
     7081 agcaggccat tagttggcgc ggtgagctaa cgcttaacgg cattaacacc gccaaagaga
     7141 tcccagagtg gccgtcgaaa ctcaatggct tgattaaaac ccgcggtagc ctgtacggcg
     7201 gcacctggca gatggaggtg ccagaactga agcttaccgg taacgtcaaa cagaacaaag
     7261 tgaacgttga cggcacgctg aaaggcaaca gttatatgca gtggatgatc ccagggcttc
     7321 atctggaact ggggccaaac agtgccgaag tgaaaggcga gctgggggta aaagatctca
     7381 atcttgatgc caccatcaac gcgccggggc tggataacgc gctgccgggg cttggcggta
     7441 cagcgaaagg gctggtgaaa gttcgcggca cggtggaagc gccacaacta ctggcggaca
     7501 tcaccgcgcg cggcctgcgc tggcaggagc taaccgtggc gcaggttcgc gtggaaggcg
     7561 atatcaaatc caccgatcag attgcgggta aactcgacgt gcgcgttgag caaatttcac
     7621 agccggatgt aaatatcaac ctcgtcaccc tgaatgccaa aggcagcgaa aaacagcacg
     7681 agttacagtt gcggattcag ggcgagccgg tttccgggca gcttaatctg gcaggaagtt
     7741 ttgatcgcaa agaagaacgc tggaagggaa ctcttagcaa tacccgcttc cagacgctgg
     7801 tcggcccgtg gtcgctgacc cgcgatattg cgctggatta ccgcaataag gagcaaaaaa
     7861 tcagcatcgg gccacactgc tggcttaacc cgaatgcgga actgtgcgtg ccgcaaacta
     7921 tcgatgcggg ggccgaaggg cgtgcggtgg tgaatctcaa ccgcttcgac cttgccatgc
     7981 tgaaaccgtt tatgccagaa accactcagg ccagcggtat cttcacgggt aaagcggacg
     8041 ttgcctggga caccaccgaa gaggggctgc cgcagggcag tatcaccctt tcggggcgta
     8101 acgtgcaggt aacgcaaacc gtcaacgatg cggcgctgcc cgtggcgttt cagacactga
     8161 atctgacggc ggaattgcgt aacaaccgtg ccgaattggg ctggaccatc cgcctgacca
     8221 ataacggcca gtttgatgga caggtgcagg tgaccgatcc gcaaggccgc cgtaatcttg
     8281 gtggcaacgt caatatccgt aacttcaacc ttgcgatgat aaaccccatc tttacccgtg
     8341 gggaaaaagc agcggggatg gtgagtgcca acttgcgtct gggtggtgat gtgcaaagcc
     8401 cgcagttgtt tggtcagctt caggttacgg gtgtggatat cgatggcaac tttatgccgt
     8461 ttgatatgca accgagccag cttgcggtca actttaacgg tatgcgctcg acgcttgccg
     8521 gtacagtacg aacccagcag ggtgaaatct acctgaacgg tgatgccgac tggagccaaa
     8581 ttgaaaactg gcgggcgcgg gtaacggcga aaggcagtaa agtgcggatc accgtgccgc
     8641 cgatggtacg aatggatgta tcgccagatg ttgtattcga ggctacacca aacctgttta
     8701 ccctcgatgg tcgcgtggat gtcccttggg cgcgcatcgt ggtgcacgat ctgccggaaa
     8761 gcgcagtagg cgtctccagc gatgtggtga tgcttaacga taacctgcaa ccggaagagc
     8821 cgaaaacggc gtcgattccg attaacagta acctgattgt ccacgttggc aacaatgtgc
     8881 gcattgacgc ctttggcctg aaagcgcggc tgacgggcga tcttaacgtc gttcaggaca
     8941 aacaagggct gggcctgaac gggcagatca acatccctga agggcgcttc catgcctatg
     9001 gtcaggatct gattgtgcgt aaaggtgagt tactgttctc tggcccgccg gatcaaccgt
     9061 atcttaatat tgaagctatt cgtaacccgg atgctacaga agacgacgta atcgccggag
     9121 ttcgcgtcac tggtctggcg gacgaaccga aagcggagat cttctctgac ccggcgatgt
     9181 cgcaacaagc tgccttgtct tatttgctac gtggacaagg gctggagagc gatcagagcg
     9241 acagtgcggc aatgacctcg atgctgattg gtctgggggt tgcgcaaagt ggccagattg
     9301 taggtaaaat cggcgagacg tttggcgtaa gcaatttagc gctcgacacc cagggagtag
     9361 gcgactcctc ccaggtagtg gtcagcggct atgtattgcc aggtctgcaa gtgaaatacg
     9421 gcgtgggtat atttgactct atagcaacac tcacgttacg ttatcgcttg atgcctaagc
     9481 tatatctgga agccgtgtct ggtgtagacc aggcactgga tttgctctat cagttcgagt
     9541 tttagcaatg cgaatatttg tctacggcag tttacgccac aaacaaggca acagtcactg
     9601 gatgaccaat gcccagttac tgggcgattt cagtatcgat aactaccagt tgtatagcct
     9661 gggccactat ccaggcgcag ttccggggaa cggaacggta cacggtgaag tttatcgtat
     9721 tgacaacgcc acgctggccg aacttgatgc cttgcgcacc aggggcggtg aatacgcgcg
     9781 ccagttgatt cagacgccgt acgggagtgc atggatgtac gtttatcaac gacccgtcga
     9841 tggattaaag ctaattgaaa gcggcgactg gttagacagg gataagtaac cctatgcata
     9901 cgccaccttc gggtggcgtt gttttttgcg agacgactcg cattctgttt tgtaattccc
     9961 tcaccttttg cttttctctc cgagccgctt tccatatcta ttaacgcata aaaaactctg
    10021 ctggcattca caaatgcgca ggggtaaaac gtttcctgta gcaccgtgag ttatactttg
    10081 tataacttaa ggaggtgcag atgcgtatta ccataaaaag atgggggaac agtgcaggta
    10141 tggtcattcc caatatcgta atgaaagaac ttaacttaca gccggggcag agcgtggaag
    10201 tgcaggtgag caacaaccaa ctgattctga cacccatctc caggcgctac tcgcttgatg
    10261 aactgctggc acagtgtgac atgaacgccg cggaacttag cgagcaggat gtctggggta
    10321 aatccacccc tgcgggtgac gaaatatggt aaagaaaagt gaatttgaac ggggagacat
    10381 tgtgctggtt ggctttgatc cagcaagcgg ccatgaacag caaggtgctg gtcgacctgc
    10441 gcttgtgctc tccgttcaag cctttaatca actgggaatg acgctggtgg cccccattac
    10501 gcagggtgga aattttgccc gttatgccgg atttagcgtt cctttacatt gcgaagaagg
    10561 cgatgtgcac ggcgtggtgc tggttaatca ggtgcggatg atggatctac gcgcccggct
    10621 ggcaaagcgt attggtctgg ctgcggatga ggtggtggaa gaggcgttat tacgcttgca
    10681 ggcggtggtg gaataaggtg tgtttattta tcgcgggcat aaaaaaaccc ttactaaccg
    10741 aagcccggcg ttcagggtta ttacgccaga agaacttatt tattctttgc gcgctcgaag
    10801 gaggcaacga tttcagcttt agcggcttct gcgttttccc aaccttcaac tttcacccac
    10861 ttgccttttt cgaggtcttt gtagtgctcg aagaagtgag cgatttgcgc tttcagcagt
    10921 tcaggcagat cgttaacgtc tttaatgtga tcgtattctt tgctcagctt gctgtgcgga
    10981 accgcaacca gtttcgcatc ttcaccggct tcgtcggtca ttttcagaac gccaaccgga
    11041 cggcaacgga tcacagaacc cggctgcagc gggtacggag ttgggaccag tacgtcaact
    11101 gggtcaccgt ccagagacag ggtgtggttg atgtaaccgt agttgcacgg atagaacatc
    11161 gcggtggaca tgaagcggtc aacgaacagt gcgccgctct ctttgtcgat ttcgtatttg
    11221 atcggatctg cgttagccgg gatctcaata acaacgtaga tgtcttccgg cagatcttta
    11281 cccgcaggga cgttgagtaa gctcatgtct gtttccttta aaaatatgtg gcaaacaagt
    11341 gccgagtatt atagccaact cgcgccgaat gtcttcgctt gttttcgtct tcattttcct
    11401 ttttcgccag ttttaagacg gtatgcagag caggaaaatc cataaccgta gccgcatttt
    11461 tcatagtgag atgaaagcga ttacaaactt gtgattaacg ttttatttac ttttttgaag
    11521 tgtgatgtaa cgcaatccgt tacataacga attgtctata gttttttcgc gaacatcttt
    11581 tcaccaataa taattactcc gacgaggaca accctatgtg gaaacgctta cttgtagtct
    11641 ctgcagtctc ggcagccatg tcgtctatgg cgttggccgc tccattaacc gtaggatttt
    11701 cgcaggtcgg atcggaatcc ggctggcgcg ccgcagaaac caatgtggcg aaaagtgagg
    11761 ccgaaaaacg cggaattacg ctgaaaattg ccgatggtca gcaaaagcag gaaaaccaga
    11821 ttaaagcggt acgttccttc gttgcacaag gggtggatgc gatctttatc gctccggtgg
    11881 tcgcgacagg ttgggaaccg gtattaaaag aggcgaaaga tgccgaaatc ccggtcttct
    11941 tgctcgaccg ttccatcgat gtgaaagaca aatctctcta tatgaccacc gttactgccg
    12001 acaacatcct cgaaggtaag ttgatcggtg actggctggt aaaagaagtg aatggcaaac
    12061 catgcaacgt ggtggagctg cagggcaccg ttggggccag cgtcgccatt gaccgtaaga
    12121 aaggctttgc cgaagccatt aagaatgcgc caaatatcaa aatcatccgc tcgcagtcag
    12181 gtgacttcac ccgcagtaaa ggcaaagaag tcatggagag ctttatcaaa gcggaaaaca
    12241 acggcaaaaa catctgcatg gtttacgccc ataacgacga catggtgatt ggtgcaattc
    12301 aggcaattaa agaagcgggc ctgaaaccgg gcaaagatat cctcacgggt tccattgacg
    12361 gtgtaccgga catctacaaa gcgatgatgg atggcgaagc gaacgccagt gttgaactga
    12421 cgccgaacat ggcgggaccc gctttcgatg cgctggagaa atacaaaaaa gacggcacca
    12481 tgcctgaaaa gctgacgtta accaaatcca ccctttacct gcctgatacc gcaaaagaag
    12541 agttagagaa gaagaaaaat atggggtatt gagggttgct atgcctgatg ccgattcgta
    12601 ggccggataa ggcgctcgcg ccgcatccgg cgatggtgca ctgaagcctg atgcgacgct
    12661 taccgcgtct tatcatgcct actgggagca cgctttacac cgggggaaac catgacgacc
    12721 gaccaacacc aggagatcct ccgcaccgaa ggattaagta aatttttccc cggcgtcaaa
    12781 gcgttagaca acgttgattt cagcctgcgc cgtggcgaaa tcatggcgct gctcggtgaa
    12841 aacggggcgg gaaaatcaac gctaatcaaa gcattaactg gtgtatacca cgctgatcgc
    12901 ggcaccatct ggctggaagg ccaggctatc tcaccgaaaa ataccgccca cgcgcaacaa
    12961 ctcggcattg gcaccgtcta tcaggaagtc aacctgctac ccaatatgtc ggtcgctgat
    13021 aatctattta taggccgcga acccaaacgc ttcggccttc tacgccgcaa agagatggaa
    13081 aagcgcgcca ccgaactgat ggcatcttac ggtttctccc tcgacgtgcg cgaaccgctc
    13141 aaccgctttt cagtcgcgat gcagcaaatc gtcgctattt gccgggctat cgatctctct
    13201 gccaaagtgc tgatcctcga tgaacccacc gccagtctcg acacccagga agtggagtta
    13261 ctgtttgacc tgatgcgtca gttgcgcgat cgcggcgtca gcctgatctt tgtcactcac
    13321 tttctcgatc aggtctatca ggtcagcgat cggatcaccg tcttacgcaa cggcagtttc
    13381 gtaggctgtc gggaaacgtg cgagctaccg cagatcgaac tggtaaaaat gatgctgggg
    13441 cgcgagctgg atacccacgc gctacagcgt gccgggcgaa cattgttgag cgacaaaccc
    13501 gttgccgcgt tcaaaaatta cggcaaaaaa ggaacgatcg caccgtttga tctcgaagta
    13561 cgccccggcg agatcgtcgg tctggctgga ttgctgggat caggacgtac cgaaaccgcc
    13621 gaagtgatct tcggtatcaa acctgctgac agcggcacgg cgttgatcaa aggcaaaccg
    13681 caaaacctgc gatcgccaca tcaggcttcg gtacttggca ttggcttctg cccggaagac
    13741 aggaaaaccg atggcatcat cgctgccgcc tcggtgcggg aaaatatcat cctcgctctc
    13801 caggcccggc gcggctggct acgtcccatt tcccgcaaag aacagcaaga gattgccgaa
    13861 cgctttatcc gccagcttgg cattcgcaca ccttcaactg aacaaccgat tgaatttctc
    13921 tccggcggca atcagcaaaa agtgttgctt tcacgttggc tactgacccg accgcaattt
    13981 ctgatcctcg atgagccaac ccgcggcatt gatgttggtg cccacgccga gatcatccgc
    14041 ctgattgaaa cgctatgcgc cgatggtctg gcgctgctgg tgatctcctc cgaactggaa
    14101 gaactggtgg gctatgccga ccgggtgctc atcatgcgcg atcgcaaaca ggtggcggag
    14161 atcccgctgg cagagctttc cgttccggcg atcatgaatg ccattgcggc gtaaggagaa
    14221 cagtgtgatg cctcaatctc tcccggacac tacgccgcca aaaaggcgct ttcgctggcc
    14281 aacgggaatg ccgcagctgg cggcactgtt gctggtgctg ctggtcgata gcctggtggc
    14341 cccgcatttc tggcaggtgg tgcttcagga tgggcgtttg ttcggtagcc ctatcgacat
    14401 tcttaatcgc gccgcccccg ttgcgctact ggcgattggt atgacgctgg tgatcgcaac
    14461 aggtgggatt gatctctccg taggggcggt gatggctatc gccggagcca caacggctgc
    14521 gatgacggtc gcgggattca gcctgccgat tgttttgtta agcgccctgg gcaccggcat
    14581 cctggcggga ttgtggaacg gcatactggt agcgatcctc aaaattcagc cgtttgtcgc
    14641 cactctgatc ctgatggtcg ccgggcgcgg cgtggcgcaa ctgatcacct ccggacagat
    14701 catcacgttt aactcgccgg atctctcatg gttcggcagt ggatcgctgt tgttcctgcc
    14761 aacgccggtc attatcgcgg tgctgacgct tatcctgttc tggctgttga cccgcaaaac
    14821 ggcgctgggg atgtttatcg aagccgttgg tatcaacatt cgggcggcaa aaaatgccgg
    14881 ggtaaacacg cggatcatcg tcatgcttac ctacgtgttg agcgggctgt gtgcggcgat
    14941 tgcgggcatt atcgtggcgg cggatattcg cggtgccgat gccaacaacg ccggattatg
    15001 gctggagctg gacgccattc tcgcggtggt gattggcggt ggttcgctga tgggcggacg
    15061 ttttaaccta ctgctttcgg tggtgggggc gctgattatt caggggatga acaccggaat
    15121 tttgctttcg ggctttccgc cagagatgaa ccaggtggtg aaagcggtgg tggtgctttg
    15181 cgtgctgatt gtccagtcgc aacgctttat cagtctgatt aaaggagtgc gtagccgtga
    15241 taaaacgtaa tttgccactg atgatcacca tcggcgtctt tgtgctgggt tatctctact
    15301 gcctgaccca gtttcccggt tttgcttcca caagagtgat ctgcaatatc ctgaccgata
    15361 atgcctttct tgggataatc gccgttggca tgacctttgt gatcctctcc ggtgggatcg
    15421 atctctccgt tggttcggtg atcgccttta ccggcgtgtt tctggcaaaa gtgattggcg
    15481 atttcggcct ctcgccgttg ctggcatttc cgctggtgct ggtaatgggc tgtgcctttg
    15541 gcgcatttat ggggcttttg atcgacgccc tgaagatccc ggcatttatc attactttgg
    15601 caggaatgtt ctttttgcgc ggcgtcagct acctcgtttc ggaagagtcg atcccgataa
    15661 accatcctgt ttatgacacg ctctcaagcc ttgcatggaa aattcctggc ggcggtcgct
    15721 taagtgcgat ggggctgctg atgctggcgg tggtggttat cggcatattc ctcgcgcatc
    15781 gtacccgctt tggtaatcag gtgtacgcca ttggcggcaa tgcgacgtcg gcaaacctga
    15841 tggggatttc cactcgcagc acaactattc gcatttatat gctctctacc ggactggcga
    15901 cactggcggg gattgtcttc tcgatttata cccaggccgg atacgcgctg gcgggcgtag
    15961 gtgtggaact ggacgctatc gcctcagtgg tgattggcgg tacgcttttg agcggtggcg
    16021 ttggaacggt attagggacg ctttttggcg tggcgattca gggactgatt cagacttaca
    16081 taaactttga tggtacgctg agctcatggt ggacgaaaat cgccatcggt attttgttgt
    16141 ttatttttat agcattacag cgtggattaa cggtgctgtg ggagaatcgt cagagttcgc
    16201 ctgtgacaag agtcaacatt gcgcagcaat aaaatcgcct ctccgtgtgg agaggcgcag
    16261 gagattacgc gtccgggaac tcacagataa agcgttcgac atcttcaacc atatggtcgt
    16321 tgccgacaaa gaatgaacgg cgctggtgca gggtttccgg gataatatcc agaatacgct
    16381 ctttgccatc gctcgcttta ccgcccgctt gttccgccag gaatgccatc gggttgcact
    16441 catacagcaa acgcagtttg ccgtccgggt ggctggcggt gcttgggtag agataaatac
    16501 cgcctttcag taggttacgg tggaaatccg caaccagtga gccgatataa cgtgaggtat
    16561 acgggcggtt ggtggattta tcttcttcct ggcagaattt aatgtacttc ttcaccccgt
    16621 tcggaaactt aatgtagttt ccttcgttga tggagtaggt tttgcctttc tccgggaagc
    16681 gcatccgttc ctggcacagg cagaaaacgc cgagcgaagg atcgtaagta aaagcgtgaa
    16741 caccgcatcc ggtactgtaa accagcatgg tagaggagcc gtataccacg taacctgccg
    16801 caacctgttt gttaccaggc tggaggaaat cttcttccgt taccggcgtg ccgacgggcg
    16861 taactcggcg gtagatggag aaaatggtac cgacagagac gttaacatcg atgttggacg
    16921 agccatccag tgggtccatc agcaccacgt attttgcatg ttcacagcct tcaaagacaa
    16981 caatctcatc ttcttcttca gaggcaatgc ctgcaacgat atcgcgtgct ttcagtgcgg
    17041 ctttcagttt ttcattagcg aacaagtcga gtttttgctg aacctcgccc tgcacgttct
    17101 cagcaccgct ggcacccagg atatcaacca gtcctgcttt gttgatatcg cgatggataa
    17161 tcttggcgcc cagttttatt gccgacagca aagcagtgag ctcaccggta gcatgagaaa
    17221 actcgtgctg cttttcgaca ataaattcac ctaacgtttt cataaaactt tccctgcaat
    17281 gtttatggag taaagcgacc gcaacaatct taacaaataa tctcaatgtt gcgctcaggt
    17341 gaatcgcgcc agcaaattac ggattatcct gaaatgcgtt tctcacttgc ccgacatatg
    17401 cgtaaaatga gcggcagatt aaaaaaggat agtgacgcat gcgcattcat attttaggaa
    17461 tttgtggcac gtttatgggc ggtctggcga tgctggcgcg ccagttaggc catgaagtaa
    17521 cgggttcgga cgccaatgtg tatccgccga tgagcacctt acttgagaag caaggcattg
    17581 agctgattca gggttacgat gccagccagc tcgacccgca gccggatctg gtgattattg
    17641 gcaacgccat gacccgtgga aatccgtgtg tggaagcggt actggaaaaa aacatccctt
    17701 atatgtcagg tccacagtgg ctgcacgatt ttgtgctgcg cgaccgctgg gtgctggccg
    17761 ttgccggtac acacggcaaa accaccaccg cgggaatggc gacttggatt ctggaacagt
    17821 gtggttacaa accgggcttt gtaatcggcg gtgtgccggg gaactttgag gtttcggcgc
    17881 gtctgggcga aagcgacttc tttgttatcg aagcggatga gtacgactgc gccttcttcg
    17941 acaaacgctc taaatttgtc cattactgcc cgcgtacgct gatcctcaac aaccttgagt
    18001 tcgatcacgc cgatatcttt gacgacctga aagcgatcca gaaacagttc caccatctgg
    18061 tgcgtatcgt tccggggcag ggtcgtatta tctggccgga aaatgacatc aacctgaaac
    18121 agaccatggc gatgggctgc tggagcgagc aggagctggt gggtgagcag ggtcactggc
    18181 aggcgaaaaa gctgaccacc gatgcttccg aatgggaagt tttgctggat ggcgaaaaag
    18241 tgggcgaagt gaaatggtcg ctggtaggcg aacataatat gcacaatggc ctgatggcga
    18301 ttgcagcggc tcgccatgtt ggtgtagcgc cggcagatgc cgctaacgcg ctgggttcgt
    18361 ttattaacgc tcgccgccgt ctggagttgc gtggtgaagc gaatggcgtc acggtatatg
    18421 acgattttgc ccatcatccg acggcgattc tggcaacgct ggcggcgctg cgtggcaaag
    18481 ttggtggtac ggcgcgcatt attgctgtgc tggagccgcg ctcgaatacc atgaaaatgg
    18541 ggatctgcaa agacgatctg gcaccttcat taggtcgtgc cgatgaagtc ttcctgctgc
    18601 aaccggcgca tattccgtgg caggtggcag aagtggcaga agcctgcgtt cagcctgcac
    18661 actggagtgg cgatgtggat acgctggcag atatggtggt gaaaaccgct cagcctggcg
    18721 accatattct ggtgatgagc aacggcggtt ttggtgggat ccatcagaaa ctactggatg
    18781 gtctggcgaa gaaggcggaa gctgcgcagt aattcggcct cagcctgaga tagtgtgcgt
    18841 aggccggata aggcgttcac gccgcatccg gcatttgagc atagtgcctg atgcgacgct
    18901 tgatgcgtct tatcaggcct acaggtgcac cgcatccggc aaaccatact cacatcaaca
    18961 acgaaaatta cccttcattc tccgccagtt cgcgcagata ctggaaaatc tggcgtgcgg
    19021 atttcggcgg cttattccct tctttctctt tcttcgcgtt gcggatcaga gtacgcagtt
    19081 gctgacgatc ggcatcgggc cacagattta atacctcagc gatggcgtca tcaccttgat
    19141 cgatcagacg atcgcgcagg ttttccagtt tatggaacag caccacctgc tggttgtgac
    19201 ggtttttcag cttatccagc gcctgacgga tcggttctac atcgcgctgg cgcagcatct
    19261 taccgatgag ctgcaactgg cggcggcgac cttccatctt aatacgctgg gcgagctcaa
    19321 tagccgcgcg cagatccgca tctaacggga tcttatccag cgcgtttttc cccagatcaa
    19381 caatttccgc gccaaggcgt tttagctcct cggcatcacg tttaatttca cttttactga
    19441 cccagataat ttcatcgtct tcgtcttcga tgtcatcacc gggaacgtcg tcgagccagt
    19501 cttcgggctg cttagtcatc tcaggctcct taaaaaaaga ggctaatgtt accagttaag
    19561 atgcgcactg aaaaacggtt ctctgttaga cttcagagaa actctctaca ttatggcact
    19621 tgcaatgaaa gtaatctctc aagttgaagc gcagcgcaag attctggaag aagcagtttc
    19681 cactgcgctg gagttggcct caggcaaatc ggacggtgcg gaagttgccg tcagcaagac
    19741 caccggcatt agcgtaagca cgcgttatgg tgaagtggag aatgttgaat tcaatagcga
    19801 tggcgcgctg gggatcactg tttatcacca gaaccgcaaa ggtagcgcat catccaccga
    19861 tttaagcccg caggccattg cccgtactgt acaggcggcg ctggatattg cccgttatac
    19921 ctcgccagat ccctgtgccg gcgtggcaga caaagagctg ctggcctttg acgcaccgga
    19981 tctcgacttg ttccaccctg cggaagtttc cccggatgaa gccattgaac tggcggcccg
    20041 cgcagaacag gcggcattgc aggcggacaa acgcatcacc aataccgaag gtggcagctt
    20101 taacagccac tacggtgtca aagtttttgg caacagccac ggcatgttgc agggttactg
    20161 ctcaacgcgt cattcgctct ccagctgtgt aattgccgaa gaaaatggcg atatggagcg
    20221 tgattacgcc tacaccattg gccgcgcgat gagcgatttg cagacgccag agtgggtcgg
    20281 ggccgattgc gctcgccgta ctttatcgcg tctgtcaccg cgtaaactct ccaccatgaa
    20341 agcgccggtc atttttgcca atgaagtggc aaccgggctt tttgggcatc tggtgggggc
    20401 gatagcgggt ggatcggttt accgtaaatc taccttcctg ctggattcgc tgggtaaaca
    20461 aattctgccg gactggctga ccattgaaga gcatccgcat ctgctgaaag ggctggcatc
    20521 gacgccattt gacagcgaag gtgtgcgcac cgagcgtcgc gatattatta aagatggcat
    20581 cctgactcag tggctgctga ccagctactc ggcgcggaaa ctggggctga aaagcaccgg
    20641 acatgcgggc ggtattcaca actggcggat tgccggacaa ggtctaagct tcgagcagat
    20701 gctcaaagag atgggcaccg ggctggtggt gacggaattg atgggccagg gcgtgagtgc
    20761 aattaccggt gattattccc gtggtgcggc gggcttctgg gtagaaaacg gcgaaattca
    20821 gtatccggtt agcgaaatca ccatcgcagg taatttaaaa gatatgtggc gcaatattgt
    20881 caccgtcggt aacgatattg aaacacgcag taatatacag tgtggttctg tgctgttgcc
    20941 ggagatgaaa atcgccggac agtaaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
    21001 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
    21061 tactcgcagt acgtgacccg gtcgaatgag cgtagtcgtc gatgaggcag tgtgaaggat
    21121 gaagtgtaaa taaaaaagga agtgagcaat gcgaaaaagc ctgttagcta ttcttgcagt
    21181 ctcctcgttg gtattcagtt cggcgtcgtt tgctgctgat cttgaagaca atatggaaac
    21241 cctcaacgac aatttaaaag tggtcgaaaa agccgataac gcggcgcaag tcaaagacgc
    21301 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
    21361 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
    21421 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
    21481 tgctgcagag caactgaaaa cgacccgcaa ttcatatcac aagaagtatc gttaattcct
    21541 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
    21601 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
    21661 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
    21721 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttgtac
    21781 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
    21841 accctggcgt ttgatacgag tgtcattcag atcgttaatg atctggtctt ccattgcttt
    21901 agtaaatggc tgaccggaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
    21961 atgaacacac ccggagacaa acagggtgca gcgagtgccg ggaccgttga cgatgtcgac
    22021 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgt
    22081 atcaggcaat caacgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
    22141 aaatgtaggc cggataaggc gtttacgccg catccggcaa tcaacgcggg atgcgacgct
    22201 ttcacatcac atccggcatt tgcttagtgc agatcaacct atctgcccat ttcccaaatg
    22261 tttaacgcgg cgcttaactt cttcctgctt gccggcgtta aacggacgtg catccgggct
    22321 acctaaatat ccgcacacgc ggcgagttac cgacacacgg gaggcgtcat ggttaccaca
    22381 tttcgggcag gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc cacactcgta
    22441 gcactcatca atcggtgtat tggtgccgta atacggtaca tgctgatagc tgtaatccca
    22501 gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa
    22561 accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac
    22621 cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg
    22681 caccacgcca aactcagcag tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg
    22741 cgtgctgtag agactgaaac cataacccgt ttcttctttc cactgatcca ctgcctgacg
    22801 cagacgttca acaatggcga tacctttcgc gcggagctgc tcgttgtcgt aaacatgttc
    22861 gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg aaatagacgc
    22921 acgaccgttt ttgaagattt cagaaatatc atcgtcagca ttgagacgca cgccacaagc
    22981 accttccata tagaggatcg gggccacgcg cgctttcacg ccttcgagac gagcgatacg
    23041 ggtcatcaga gccttacgtg ccagcaccag acgttcatcc agcagcttcc agaaggtggc
    23101 ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca cgccgaggtt
    23161 gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga ggaagctgcg
    23221 gcagcccatc ggggttttaa acgaaccggt gactttcact acctgatcgt agttcaggat
    23281 atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg
    23341 atcgcctttt ttatggttca gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc
    23401 agttttacgg tttttaccga ggcctgcgat acggttacgc aggatagatt cctgaatcag
    23461 gcgcgactcc cagctggtgc ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt
    23521 ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt agcactcttt
    23581 aatggtacga gagttagcgt agccttcggc gtccgggatg ttccactctt ctgcggtctt
    23641 gcgatgtttg ttgtagctgg cggtgacaaa cggtgccagc acttcatcga tacggttaat
    23701 ggtggtgccg ccataaatat ggctggcaac ctgagcaata atctgcgcag ttaccgcggt
    23761 tgccgtagag atcgacttcg gcggttcaat ctcggcgttc cccatcttaa agccctgggt
    23821 cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaaaaacg gtgagtaatc
    23881 gagatcgtga tagtgaatat cgccacgctc atgtgcctgc accacgtcac gcggcagcag
    23941 gtgctgacgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct gggttggaat
    24001 caccttgctg tctttgttgg cgttttcgtt gagtaacgag gcgttggtct gctcgaccag
    24061 accacggatc tcctgattca ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg
    24121 gtactcgatg taagcacgag ccagttgttt gtatggacct gacatcagct ggttttcaac
    24181 agcggtctgg atctcattga tatccacctg gttgcggccc tgcatctgct cgctgacaac
    24241 tgcagcaaca gtggcgcaat aatcggcatc atcgacttcc gctgctttag ctgcacgcag
    24301 aatcgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc gtttcatcac
    24361 atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa attgggaaag
    24421 gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc
    24481 cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga ttgatacaac
    24541 atattgggtt gggacgcatt ttaaagtcta tatatagtgc tttgcatcaa ggatgtttga
    24601 actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg cgctacagcg
    24661 ttatgtaaat tttttaatga atttgctggt tgaaaaatca acaaaaacaa catgctgaca
    24721 gacaaaaccc cggaatgacc ggcattaccg gggcttaggg aagatttact tctgtaacca
    24781 ccagacagcc tcaaaaggcc gtaaagtcat ggcacagggt tgtggtgagg cttcttcgta
    24841 gttatgcatc acaagctgcc agttgccgcg catttgccct ggctgccagg gttggatctc
    24901 acggctaagg ttggcaatga ccagcaaggt ttgccccttc cattcacggc gatagcacca
    24961 caatacaggg ctgtttggca gcagatcctg gtaattgccc catgtcagga tggcttcctg
    25021 cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggactcat cggccagcgc
    25081 ggcttctacg ttgatttgtt gatagttatc gcccaggcca atccacggtt cgccagccgt
    25141 aaacccggca ttatcgccgt tgctccattg catgggcgtg cggctgttgt cacgggattt
    25201 actggcgagg attgccaata actcgtcggc atcacgccca tcgttgcgca gctcggcaaa
    25261 catattgagg ctctctacgt cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat
    25321 gccaatctct tcgccctggt agatatacgg cgttccctgc atgccatgca gcaccatcgc
    25381 cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag aaacaatgcg
    25441 cggctgatcg tggttacacc agaacaaggc attcaatgct acgttgtgca ttccttgttg
    25501 ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc
    25561 accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc
    25621 tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt
    25681 cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg
    25741 tggcccgtcg gtgtagaagc gacgcccgtc gccatccagg tcatcaggga aacgcgggtc
    25801 tttggagatc agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa
    25861 ctcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg
    25921 ttctggtgca aagagatgca aatagtactg ttcgctttcc gcatgccagc gccacgcact
    25981 accgccaaat tttgaacgcc agttgttcgg tggcgtttct ggttcgccat cgcgccagat
    26041 ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt
    26101 agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt
    26161 caccagttcg tcaaaatcgt ccagcgtgcc gtaggtggga tcaatcgccg tatagttcgc
    26221 tacatcgtaa ccgttatcga cctgcggaga gacataaaag ggggttagcc agatggcatc
    26281 aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc
    26341 gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca
    26401 ccagtgggga agattagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc
    26461 ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta tttccgctga tagataaacg
    26521 aggtgagtac aatcgggatg atgatggcga tagccattgc cagcgcaaac acctgccagt
    26581 agctcggttg aatcgagaga attcccggca ggccgcctac gccgatgcca ttcgccataa
    26641 cgccgttcag gccgcatagc aatcctgcca gaccagaacc aatcatcgcg cacagcatcg
    26701 ggaagcgata tttcaggttg atgccgtaca ttgcaggctc agtgacccca agccaggcgg
    26761 agatcgccgc aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatgc
    26821 ctatcacggc ggagccctga gcgatattcg acagcgcaat cagcggccac actggcgtac
    26881 cacccatgct ttgaatcatc tgcaaatcaa tagcaagcgt ggtctggtgt acaccggtga
    26941 tcaccagcgg ggcgtacagg aagccaaaca atgccgcgcc aatcggagca aagctgccgg
    27001 tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa
    27061 tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat
    27121 agaggtaatc cggcacgatg cgtttaaggc gagtttcaat aacgcccagc gccagcccgg
    27181 ctaacagtgc cgggatcacc tgcgcctgat agcccacttt ggcgatgctg aacatgccaa
    27241 agtcccacac ttccggcagc tgctgcccga gcagataagc gttcatcagt tggggggaaa
    27301 ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc gcccattttt ttcaccgctg
    27361 accagcaaat cccgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat
    27421 cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ttgaccgttg ctcatgggca
    27481 aatcgccgat cacattgcga aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca
    27541 acgggaagaa gatctctgcg aaatgagaga tcaactgctc atgccatttc atattctgcc
    27601 gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac gctatcagtg
    27661 cttgatagta atcacccacg ttggtgccaa tcaccacctg aaattgcccg gcattggtga
    27721 aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt
    27781 tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc
    27841 cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc
    27901 atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg
    27961 gaacgttccc gaaacgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg
    28021 ttggcgggga tgatgatttg ttgcggttcg ctgcgttcgg ttacctgcgc gatcaactgg
    28081 caagccgcct ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat ctccggatga
    28141 aggaatttca ttaacggcgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc
    28201 tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta
    28261 gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc aagccctggc
    28321 agggcggcaa cgggatgcag tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt
    28381 cgcttaccgg ttgtcacgtc actgtgcggc acgccgagat aactgatatt acgatgcccc
    28441 tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa
    28501 gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt
    28561 tcttctgtta tgccagtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc
    28621 acccccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat gattgggtcg
    28681 taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa
    28741 tccagacggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca
    28801 gaacgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc gcgggtgcgc
    28861 tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt
    28921 aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg taacgtgttg
    28981 tttaattatt tgagcctaac tttacccgtg cattcagcaa tgggtaaagt ctggtttatc
    29041 gttggtttag ttgccagcag gtattatatc gccatagatg ctacgaatat tattggattc
    29101 tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg
    29161 cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg
    29221 gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc
    29281 ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt
    29341 ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt catcgtgatc
    29401 cattgcctgg cgcgcagcag acagtgaata cggtggtccc gccgtcctta agtgcgcatt
    29461 gcctgaaaat ggcggtgatg cccgaagaag aattgtggaa aacgttcgac acccatccgg
    29521 aagggttaaa tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac
    29581 ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta
    29641 atattttact caccattctc ggcgctattt cttacgccac ggaagattta tttgccgcgg
    29701 gcgttatcgc gctaatggtc gctatttcaa cgttgctgaa ctttattcag gaagcacgtt
    29761 ccactaaagc ggcagatgcc ctgaaagcga tggtcagcaa taccgcgacg gtgctgcgcg
    29821 taattaacga caaaggcgaa aatggctggc tggagatccc gatcgaccag ctggtgcccg
    29881 gcgatattat aaaactggcg gcgggagata tgatcccggc agatttacgt atcttgcagg
    29941 cgcgggatct gttcgtcgct caggcgtcgt taaccggtga gtctctgccc gtggaaaaag
    30001 ccgctaccac tcgccagccg gagcacagca atccgctgga gtgcgacacg ctgtgtttta
    30061 tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgctaca ggtgccaata
    30121 cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc
    30181 agcaagggat cagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggcaccgg
    30241 tggtgctgtt aatcaatggc tacaccaaag gcgactggtg ggaagcggcg ctgtttgcgc
    30301 tttcggtcgc ggtaggccta acgccggaaa tgttgccgat gattgttacc tcgacgctgg
    30361 cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt caaacatctg gatgctattc
    30421 agaactttgg cgcaatggat attctgtgca ctgataaaac cggcaccctg acacaggata
    30481 aaattgtgct ggagaatcat accgatatct ccggtaaaac cagcgaacgc gtgctgcata
    30541 gcgcgtggtt gaatagtcat tatcagaccg gacttaaaaa cctgctcgat acagcggtgc
    30601 tcgaaggtac ggatgaagag tcagcgcgct cgctggccag tcgttggcaa aaaatcgatg
    30661 agattccgtt tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa aataccgagc
    30721 accatcagct ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc
    30781 gtcacaatgg cgagattgtg ccgctcgatg acatcatgct gcgtaagatt aagcgggtta
    30841 ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag
    30901 cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg
    30961 ctttccttga tccgccaaaa gagacaaccg ctccggcact gaaggcatta aaagcgagtg
    31021 gaattaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg
    31081 aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg
    31141 acgaactggc aaatcttgca cagcgcacta cgctgtttgc ccgcctgacg ccgatgcata
    31201 aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg
    31261 gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg
    31321 tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg
    31381 aagagggggt tattgaggga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga
    31441 cggcgagttc taacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct
    31501 tcctgccgat gttgccgtta cacttgctta ttcagaacct gctgtacgat gtgtcacagg
    31561 tggcgatccc gtttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc
    31621 cggcggatct ggggcgcttt atgatcttct tcggaccgat cagctcgatc ttcgatattc
    31681 tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt
    31741 tccagtcggg atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc
    31801 gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg
    31861 tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcaat
    31921 tacaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca gggtatatga
    31981 cattaaccca gttggtgaaa gggttctata gccgtcgtta cggctggcaa taaagaataa
    32041 actgggcacg atagccccga ttcagattgc agacaaagta agctttgttc atgccggatg
    32101 cggcgtaaat gccttatccg gccaacaaat tcaataaatt gcaggaatca cgtaggcctg
    32161 ataagcgtag cgcatcaggc gattttgcgt ttgtcatcag acttaatccg ggcatgatag
    32221 cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa tcttcacgtc
    32281 tttcggcaga cgggcaactt caacgcaaga acgtgccggg aaggtggcgt tgtgttcggt
    32341 gaagaaggct tcgtaagtgg cgtttacggt tgcgaagtcg ttcagatctt ttacaaacac
    32401 ggtagtttta acgatgtcgc ccactttcag accagcggct tcgacgatcg cttttacgtt
    32461 atccagcgac tgacgtgcct gtgcagcgac gtctgccggt acttcgcccg ttttcggatt
    32521 taccgggatc tgaccggaag tgatgatcat attgcccaga tcaacgccct gtacgtaagg
    32581 accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt ctcctttatt
    32641 acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca accgcaatta
    32701 attggccagc accacattat gggaaaactc tttttcacag tatttgcatt tgagcgcgat
    32761 atcattggcg cgttttcgca cggcaaagct ggaagaaacc ggttcggcat ggctgataca
    32821 gttgctgttc gggcagacca gcacattgtc gatgcgctcc ggcagacttg ggcgcgattt
    32881 acccaccact tcatagttgt cgatacggtt aaccgtggct tgcggcgcat acaatgccag
    32941 ttgatctacc tggtcttcac tcaaaaaggt attttcgatt ttgatcagat ctttgcggcc
    33001 catctcgcca gaaggcaggt tcagaccgat ggtgatgcgc tgatccgttt cggtcagctt
    33061 gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta ccgtgccgcg
    33121 tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc cttacagtac
    33181 cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc cgttgcctgc
    33241 ctgctggaag taccaggcgt gtggcgtttt atcaacatcc gtcgcaatct catcaacacg
    33301 cggcagcgga tgaagtactt tcatattggc tttggcgttg tggagatcgc tggcgcgaag
    33361 aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt tttgcacgcg
    33421 ggtcatgtac aggatgtcaa cttccgccat cacttcttca atagagctgt gcagactcca
    33481 tgcgatccct ttttcatcaa gcatatccag aatgtattgc ggcattgcca gcgcgtccgg
    33541 cgcgatgaag taaaaacggt tgccgtcgaa cttcgctaac gcctgagtca gggagtgaac
    33601 ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca gacgcccctg
    33661 ggtttcctga atggtgaata agtccagcaa ggtttgcgtc ggatgttggt tggagccatc
    33721 accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg ccgcaccttc
    33781 ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa tggtatcggc
    33841 cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc ccaccacgct
    33901 ggcacccagg cggtgcatgg aagtttcgaa agagaggcgg gtacgggtag aggcttcgaa
    33961 gaaacagctg gcaatgactt tgtgcttcaa cagctctggt tgcgggtttg ctttcagttt
    34021 cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta tggaaatgat
    34081 atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc aaaaaaaagc
    34141 ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca ggccagcgtc
    34201 ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc ctcccggcaa
    34261 attgtccggc attatactca tcgtcagaag cggtgcaagc atttgatgca atattttgtc
    34321 agcgcaaacg gtttatttga attataagtc aaaatatatg tatttttatt tactgtgatt
    34381 ctggagggga actgtcgttc ggtatcaata attgcagaca tttcctgctt ttttcttttt
    34441 cactttcacg caatcagata tgcattttat tccattcatc cgacttatag ggcggagtgt
    34501 gaaaacgaac ggctaacact attgcttaca gctcagggat gcgcgctatc actttaattt
    34561 caaaatcaaa gcctgccagc catgtaacgc ccaccgccgt ccaggttgga taaggtggcg
    34621 cgctaaatat ttcatttttc accgtcatga tgtcttcaaa ttgtttttct ggatcggtat
    34681 gaaagctcgt aacatcaatg atatcgtcaa aagtgcatcc cgcagctgcc agggtcgcat
    34741 gcaaattatc aaatgccagt ctgacttgtt gctgaaaatc gggttctggt gttccgtcct
    34801 ctcgacttcc tacttgcccg gaaacaaaca gcaaatcgcc ggaacgaata gccgcagaat
    34861 aacgatgttc tgcatacagt gaatgtcggc cagcagggaa aacagcggtt ctttctacca
    34921 tttggttatc ctcaagattt acgacatgaa cagaagattt atatttacca ggagccgctt
    34981 ttagcggacg acgtgagtaa acaaaaccca gacatcatgg gaaatggctg ggcttaattg
    35041 agcgtagtcg gttatgcgcc aaacgcgcca tcaatggtat gcatcgcgcc ggtaacaaaa
    35101 ctggcttctg gccctgctaa ccatgcgacc ataccagcga cctcttccgg ttgcccatgt
    35161 cttttgatag ccataaaact atgcaacata tcgcgcattg gcccgttggc gggattagcg
    35221 tcggtatcaa ttggccctgg ctggacgacg ttgatggtga tcccacgcgg tccaaaatca
    35281 cgggccagcc cgcgcgccat gccttgtagg gcagatttac tggcggcata agcagccatg
    35341 cctgcaacag gcatacgatc gccattcacg gagccgatga ttaagatgcg cccaccttcg
    35401 ggcatctgcc gggcggcttc aacagaggca tggtaaggag catgaatatt gattttgaaa
    35461 aggcgatcaa tatcgtcggc atttaattcc agggcatcgc caaagacgcc aatacctgca
    35521 tttaccacca ggatatccaa tgcgccgctc ttacgaacga catcaatgac agcgtctctg
    35581 tcagcactat ctgtaaatac tgctgtcgct cccgtctctt gtgccaggcg ttcagcggca
    35641 tctttcgacc ccgcataggt gaatcgtaca ttggccccat cggtgacgaa acgacgtacg
    35701 atagcggcac cgattccacg actgccaccg aggatgagaa ctgtcttacc tgtaaaagcg
    35761 cccataagga ctccttgatt tattatgtaa catgcattac aaaactgttt taactttctg
    35821 tcaacaagtt ttgtaatggt cactaaaaaa caatctcgcg ttccaggtcg tcccagacgt
    35881 ttcgctcctg agcaggcagt ctctgcggca aaagtgcttt ttcaccaaaa aggtttcgat
    35941 gctgtcagtg ttgctgaagt tactgattat cttggtatta accccccgag cctctacgcg
    36001 gcttttggca gtaaggctgg gttatttagc cgtgtactca atgaatatgt cggtacggaa
    36061 gctattccgc ttgtcgatat tcttcgtgat gatcgtccgg taggggagtg cctggctgag
    36121 gtattaaaag aagcggcgcg cagatatagc caaaacggcg gctgcgctgg ctgtatggtt
    36181 cttgaaggca ttcatagtca tgatccacaa gcgcgtgata ttgccgttca atattatcac
    36241 gccgcagaaa cggccattta tgactatatc gccaggctgc atccacaaag cgcacaatgt
    36301 gtgactgatt ttatgagtac cgtgatgtca gggctttctg cgaaggcacg agaggggcac
    36361 tccctcgaac aactctgtgc aactgctgca ctggcggggg aagcgataaa aactattctc
    36421 aaggagtgat gctggccttg atccgaaagg cgggaacgcg cctgccgata agttgtgata
    36481 agacaataat tcacgcgtta aggctggctg aattgatcat cttttcgtat aacggtggaa
    36541 atgaaacatt gttttaatta aggagtggaa aagatgatca tcggaaatat tcataatctt
    36601 cagccgtggc taccccagga gttacgccag gcgattgagc atatcaaagc acacgttacg
    36661 gcagaaacgc caaagggcaa gcacgatatc gaaggtaatc gtctgtttta tcttatctcg
    36721 gaagatatga ccgagccgta cgaagctcgc cgtgcggagt accatgcccg ctatctcgac
    36781 attcagattg tgttaaaagg tcaggaaggc atgaccttca gcacacaacc ggcaggtacg
    36841 ccggataccg actggttagc tgataaagac atcgcatttt tgccggaagg cgttgatgag
    36901 aaaacagtta tccttaatga aggtgatttt gttgtgtttt atccggggga agtgcataaa
    36961 ccgctgtgcg cagtgggtgc accagcccag gttcgcaaag cagtagtgaa gatgctgatg
    37021 gcgtgatgac ttttcgccgt aaataactcc aggtttacgg cgagtttgtg aaaagagcgt
    37081 tttttgatat ttttttgtga gtaaaatttg taatgcttag acgttcttat taactcaagg
    37141 agttcgtcat gagcaaaata tcagatttga attattctca acacattaca ttagccgaca
    37201 attttaaaca aaaaagtgaa gttttaaata cctggcgtgt tggaatgaat aattttgccc
    37261 gtaatgccgg ggggcaggat aacacaagaa atatccttaa tcctaagaca tttttggagt
    37321 ttttggtaaa aatatttacc ctgggttatg tggattttag caaacgctcc aacgaagcgg
    37381 gaag
//
LOCUS       DAZFKY010000025        35450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFKY010000025 DAZFKY010000000
VERSION     DAZFKY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            121..660
                     /gene="elfA"
                     /locus_tag="ACHKCL_001135"
     CDS             121..660
                     /gene="elfA"
                     /locus_tag="ACHKCL_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5762150.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            743..1444
                     /gene="elfD"
                     /locus_tag="ACHKCL_001136"
     CDS             743..1444
                     /gene="elfD"
                     /locus_tag="ACHKCL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5762151.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1469..4068
                     /gene="elfC"
                     /locus_tag="ACHKCL_001137"
                     /pseudo
     CDS             1469..4068
                     /gene="elfC"
                     /locus_tag="ACHKCL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4059..5129
                     /gene="elfG"
                     /locus_tag="ACHKCL_001138"
                     /pseudo
     CDS             4059..5129
                     /gene="elfG"
                     /locus_tag="ACHKCL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5157..5684
                     /gene="ycbU"
                     /locus_tag="ACHKCL_001139"
     CDS             5157..5684
                     /gene="ycbU"
                     /locus_tag="ACHKCL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5762152.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5692..6207
                     /gene="ycbV"
                     /locus_tag="ACHKCL_001140"
     CDS             5692..6207
                     /gene="ycbV"
                     /locus_tag="ACHKCL_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5762153.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6173..6909
                     /gene="ycbF"
                     /locus_tag="ACHKCL_001141"
                     /pseudo
     CDS             6173..6909
                     /gene="ycbF"
                     /locus_tag="ACHKCL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            7020..8030
                     /gene="pyrD"
                     /locus_tag="ACHKCL_001142"
     CDS             7020..8030
                     /gene="pyrD"
                     /locus_tag="ACHKCL_001142"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5762154.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8204..8746
                     /gene="zapC"
                     /locus_tag="ACHKCL_001143"
     CDS             8204..8746
                     /gene="zapC"
                     /locus_tag="ACHKCL_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5762155.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8743..9852)
                     /gene="ycbX"
                     /locus_tag="ACHKCL_001144"
     CDS             complement(8743..9852)
                     /gene="ycbX"
                     /locus_tag="ACHKCL_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5762156.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10096..12204
                     /gene="rlmKL"
                     /locus_tag="ACHKCL_001145"
     CDS             10096..12204
                     /gene="rlmKL"
                     /locus_tag="ACHKCL_001145"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5762157.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12216..14123
                     /gene="uup"
                     /locus_tag="ACHKCL_001146"
     CDS             12216..14123
                     /gene="uup"
                     /locus_tag="ACHKCL_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5762158.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14253..15506
                     /gene="pqiA"
                     /locus_tag="ACHKCL_001147"
     CDS             14253..15506
                     /gene="pqiA"
                     /locus_tag="ACHKCL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5762159.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15511..17151
                     /gene="pqiB"
                     /locus_tag="ACHKCL_001148"
     CDS             15511..17151
                     /gene="pqiB"
                     /locus_tag="ACHKCL_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5762160.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17148..17711
                     /gene="pqiC"
                     /locus_tag="ACHKCL_001149"
     CDS             17148..17711
                     /gene="pqiC"
                     /locus_tag="ACHKCL_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5762161.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17967..18134
                     /gene="rmf"
                     /locus_tag="ACHKCL_001150"
     CDS             17967..18134
                     /gene="rmf"
                     /locus_tag="ACHKCL_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5762162.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18204..18722)
                     /gene="fabA"
                     /locus_tag="ACHKCL_001151"
     CDS             complement(18204..18722)
                     /gene="fabA"
                     /locus_tag="ACHKCL_001151"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5762163.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18791..20551)
                     /gene="ycbZ"
                     /locus_tag="ACHKCL_001152"
     CDS             complement(18791..20551)
                     /gene="ycbZ"
                     /locus_tag="ACHKCL_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5762164.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20737..21189
                     /gene="matP"
                     /locus_tag="ACHKCL_001153"
     CDS             20737..21189
                     /gene="matP"
                     /locus_tag="ACHKCL_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5762165.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21265..22305)
                     /gene="ompA"
                     /locus_tag="ACHKCL_001154"
     CDS             complement(21265..22305)
                     /gene="ompA"
                     /locus_tag="ACHKCL_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5762166.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22662..23171)
                     /gene="sulA"
                     /locus_tag="ACHKCL_001155"
     CDS             complement(22662..23171)
                     /gene="sulA"
                     /locus_tag="ACHKCL_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5762167.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23390..24019
                     /gene="sxy"
                     /locus_tag="ACHKCL_001156"
     CDS             23390..24019
                     /gene="sxy"
                     /locus_tag="ACHKCL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5762168.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23982..26144)
                     /gene="yccS"
                     /locus_tag="ACHKCL_001157"
     CDS             complement(23982..26144)
                     /gene="yccS"
                     /locus_tag="ACHKCL_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5762169.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26154..26600)
                     /gene="yccF"
                     /locus_tag="ACHKCL_001158"
     CDS             complement(26154..26600)
                     /gene="yccF"
                     /locus_tag="ACHKCL_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5762170.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26723..28777
                     /gene="helD"
                     /locus_tag="ACHKCL_001159"
     CDS             26723..28777
                     /gene="helD"
                     /locus_tag="ACHKCL_001159"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5762171.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28809..29267)
                     /gene="mgsA"
                     /locus_tag="ACHKCL_001160"
     CDS             complement(28809..29267)
                     /gene="mgsA"
                     /locus_tag="ACHKCL_001160"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5762172.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29363..30025)
                     /gene="yccT"
                     /locus_tag="ACHKCL_001161"
     CDS             complement(29363..30025)
                     /gene="yccT"
                     /locus_tag="ACHKCL_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5762173.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30198..30611
                     /gene="yccU"
                     /locus_tag="ACHKCL_001162"
     CDS             30198..30611
                     /gene="yccU"
                     /locus_tag="ACHKCL_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5762174.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLDLAK"
     gene            complement(30656..30973)
                     /gene="hspQ"
                     /locus_tag="ACHKCL_001163"
     CDS             complement(30656..30973)
                     /gene="hspQ"
                     /locus_tag="ACHKCL_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5762175.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31031..32221)
                     /gene="rlmI"
                     /locus_tag="ACHKCL_001164"
     CDS             complement(31031..32221)
                     /gene="rlmI"
                     /locus_tag="ACHKCL_001164"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5762176.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32316..32594
                     /gene="yccX"
                     /locus_tag="ACHKCL_001165"
     CDS             32316..32594
                     /gene="yccX"
                     /locus_tag="ACHKCL_001165"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5762177.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32591..32920)
                     /gene="tusE"
                     /locus_tag="ACHKCL_001166"
     CDS             complement(32591..32920)
                     /gene="tusE"
                     /locus_tag="ACHKCL_001166"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5762178.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33011..33670)
                     /gene="yccA"
                     /locus_tag="ACHKCL_001167"
     CDS             complement(33011..33670)
                     /gene="yccA"
                     /locus_tag="ACHKCL_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5762179.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34078..35097)
                     /locus_tag="ACHKCL_001168"
     CDS             complement(34078..35097)
                     /locus_tag="ACHKCL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5762180.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35075..35317)
                     /locus_tag="ACHKCL_001169"
     CDS             complement(35075..35317)
                     /locus_tag="ACHKCL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5762181.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         9137 a         8419 c         9176 g         8718 t
ORIGIN      
        1 agatttgtca cttattggat ttagtatgct tgatttaaat ggcggacaat tattgttatt
       61 attaagtcta atgtcattta aaggcattat catatttacg aagagatcag gatgataacg
      121 atgaaaaaaa gtgtattgac ggcgtttata actgtggtat gtgcaacgtc cagcgttatg
      181 gctgctgatg ataatgctat cacggatggc tcagtaacat ttaatggtaa agttattgct
      241 ccagcttgta ccctggtagc tgcgacgaaa gattccgtgg tgactttgcc agatgttagt
      301 gccacgaagt tgcaaaccaa tggtcaggtt tctggcgtgc aaactgatgt gccaattgaa
      361 ttaaaagatt gtgatactac cgtaacaaaa aatgcaacgt tcacctttaa tggcactgcg
      421 gatactactc agattacagc gtttgctaac caggcctcat ctgatgctgc tacaaacgtg
      481 gccctgcaaa tgtatatgaa tgatggtaca acggccatca agccagacac agaaaccggg
      541 aacattttgt tgcaagatgg agatcagacg ttgactttta aagttgatta tatcgctacg
      601 gggaaagcga cttctggtaa tgtgaatgcg gtaacaaatt tccatattaa ctattattaa
      661 tagaactcat taattgtttt attaattagt acccctccag tgttatggag gggatattca
      721 tattttttaa gagtgactat ttatgaaaac ttgcataaca aagggaattg tgaccgtaag
      781 tttaacggca atattactca gctgttcgtc cacatgggcg gcaggtaaag gcggggttgg
      841 acttgcagca acacgtcttg tttattcgga aggtgaagag caaatttcac tgggtgtacg
      901 taacaccagt ccggatgttc cttatctgat tcagtcatgg gtgatgaccc cagataataa
      961 aaaatcagcg gactttatta ttacaccacc gctatttgtg ctgaatccgg caaacgagaa
     1021 tctgttacgc attatgtaca ttggcgcgcc gttggcgaaa gacagagaaa ctcttttctt
     1081 cactagcgta cgggcagtcc cttcaacaac gaagcgggaa gaaggcaata ccctgaagat
     1141 tgccacacaa agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt atcccttagg
     1201 cgaggctccg gcgaaactgc gttgcacttc gtcagctgac atggttacgg tcagtaaccc
     1261 aacaccttat ttcattaccc tgacagacct gaaaataggt ggaaagttag ttaaaaatca
     1321 aatgatttcc ccctttgata aataccaatt ctctctgcca aagggtgcca aaaatagcag
     1381 cgtaacgtat cgaaccatca atgactacgg ggcggaaacg ccgcaactca actgtaaatc
     1441 gtaattcgtc ttcagttaag agagcgagat gtatagaact caccgacaac acagcctgtt
     1501 aagctctggt ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt
     1561 caatgcacaa gctgaaacct ggttcgatcc agcctttttc aaagatgatc cctcaatggt
     1621 ggccgatttg tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga
     1681 tattgttctg aatcagacaa ttgtagatac gcgcaacgtc aattttgttg agataacgcc
     1741 agagaagggg attgccgcct gtttgacgac tgaaagcctg gatgcaatgg gcgtgaatac
     1801 tgatgcattt ccggctttta aacaactgga caaacaagcg tgtgcgccat tggcggagat
     1861 tattccggat gccagcgtaa cttttaatgt gaataaactc cgtctggaaa tttcagtacc
     1921 gcaaattgcc atcaaaagta atgctcgtgg ttatgtcccc ctgaacgttg ggatgaaggg
     1981 atcaacgcgc tattactggg atattcattt agcggggcta acagtattca tagcagcgca
     2041 gacagtgatt ctggcgacag ttattttctg aatttaaaca gtggcgttaa tttaggccca
     2101 tggagattgc gcaacaattc aacatggagt cgtagtagtg gccaaaccgc agaatggaag
     2161 aatctcagca gctatttgca acgggcggtt attcctctga aaggcgaact gaccgtaggt
     2221 gatgattata ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg
     2281 tcagatgaca acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc
     2341 gccaaaagca atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat
     2401 gtatcgcctg gtgcttttga aattagtgat atctattcca cgtcgtcgag cggtgatttg
     2461 ttagttgaaa tcaaagaagc ggacggcagc gtcaatagct acagcgtacc cttttccagc
     2521 gtgccattac tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga
     2581 accaatagta atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga
     2641 ccgtggggaa cgacctggta tggtggtgga caatatgctg aatattaccg tgccgccatg
     2701 tttggtctgg gatttaacct cggcgatttc ggagcaattt cgttcgatgt gacccaggcg
     2761 aagagtacgc tggcagacca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc
     2821 aaaacgctca accaattggg tactaacttt caattgatgg gctatcgcta ttcgacgtcg
     2881 ggtttctaca ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac
     2941 ggtgatgatg aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt
     3001 ggcaaactgc aggtcaatat ctcccagcaa ttaagcgagt acggttcgtt ttatttaagt
     3061 ggtagccagc aaacttactg gcataccgat caacaggatc ggctattaca gtttggctat
     3121 aacacgcaaa ttaaagatct ctcgctgggg atctcctgga actacagtaa gtcccgtggc
     3181 caacctgatg ctgatcaggt gtttgcactt aatttttccc tgccactcaa tctgttgctc
     3241 tccagaagta atgatagcta taccagcaaa aaaaattacg cctggatgac ctctaacacc
     3301 agtatcgata acgaagggca cactacacaa aacctgggtt taacggagac actactcgat
     3361 gacggtaacc tgagctacag cgtgcaacag ggatataaca gcgaggggaa aacggctaat
     3421 ggcagcgcca gcatggacta caaaggggtg tttgcagatg cccgagtggg ctacaactac
     3481 agcgataacg gcagtcaaca acaactgaac tacgctcttt caggcagttt agttgcccat
     3541 tcacagggca ttaccctggg gcaatcactg ggggaaacta acgttctgat tgcagcacca
     3601 ggcgcagaga atactcgtgt ggcgaacagc accgggctga aaactgactg gcgcggatat
     3661 accgttgttc cttatgccac ttcttatcgt gaaaatcgaa tcgcacttga tgcggcgtcg
     3721 ttaaaacgta acgtggatct tgaaaatgca gtagtcaacg tggttcccac caaaggggcg
     3781 ttggttctgg cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag
     3841 cagggtatat cgctgcgttt tggcgcgata gcgacgctgg acggcgtaca gactaatagc
     3901 ggcataattg atgatgatgg ctcgctctat atggcgggtt taccggcgaa gggaacaata
     3961 accgtgcgct ggggcgaagc tcccgatcaa atttgtcata tcagttacga gcttaccgaa
     4021 caacaaatta actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa
     4081 aatgcgtgtc attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa
     4141 ccgctgctta tagcgggcag tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt
     4201 ttggcgttaa aaccctggag gaagaagaaa atacgactgg ggtagtaaaa gacaaatttt
     4261 atcagtggaa cgaatcgaat gattattatg tttcctgtga ttgcgataaa gacaatgtca
     4321 gaagtggccg atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt
     4381 acaaaattaa tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta
     4441 cagcggttcc tttcgaaaac gtggggactg gggcagatac ccggtggcat atttgtgacc
     4501 ccggcggtca acgtttaggc ggccagggag ctagcggtaa tagcggtagc ttttccctga
     4561 aaatattgca gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg gcgcgattat
     4621 ttgaatgcta caacataccc gcaggtgatt cctgcacgac tacaggcaca ccggttttag
     4681 tgtattacct gtctggtact atcaattcac ttggctcatg ttccgtcaat gccggagaaa
     4741 caatcgaggt cgatctgggc gacgtatttg cggctaactt tcgtgttgta gggcataagc
     4801 ctcttggggc cagaacggca gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg
     4861 ggttagttaa cgtcaacctg agtctgacgg caaccacaga ccccagctat ccccaggcga
     4921 ttaagacgtc acgtcctggc gtgggcgtgg tggtgaccga tagccagaac aacattattt
     4981 cccctgctgg tggaacatta ccgctctcta ttcctgatga tgcagacagt atcgcgtgaa
     5041 tgaatgtcta tccagtcagc acgacaggtg taccaccaga aaccgggcga tttgaagcca
     5101 cggcaacggt gagaataaat tttgattaac gcgtgaacgt atgaagaaaa aaaacgatat
     5161 ttcagtgcgt tatcttgttc tttagcattc ttaacatcca tgtcgggatg gctgggcctg
     5221 aacaagttag tatgcatatt tatgggaatg tggtcgatca gggctgtgat gtcgccacca
     5281 aaagtgcatt acaaaatatt catattggtg attttaatat cagtgatttt caggccgcga
     5341 ataccgtaag cactgctgct gatttgaata tcgatatcac cggttgtgcc gctggtatta
     5401 ctggcgcgga cgtccttttt agcggcgagg ctgacaccct tgcgccgaca ctgctcaaac
     5461 taactgacac aggcggaagc ggtggtatgg caacggggat tgccgtgcaa attcttgatg
     5521 cgcaaagtca gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg
     5581 gggataacac actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg
     5641 gtaatgcgac ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa
     5701 cggataatct ggattctgtt cttactggga ttaacggggg ggtgtgagct atttgcccat
     5761 gatggcacgg tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat
     5821 agcaagcaaa tcaatgtgca gttaggcgat gtctcactga cacgtttttc tcatgggaat
     5881 tatggcccgg agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg
     5941 gttgacgtca ccttttcagg aacccccgac ggagtgcaga gcgaaatgtt gtccattgaa
     6001 agtggtacgg atgcagccag cgggctggca attgcgattc tggatgatgc gaagatattg
     6061 attccgctta accaggcgag taaagactac agcctacata gcggtaaggt gccattaact
     6121 ttttatgcgc aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc
     6181 gcgacatttg tacttcatta tgactaatac gtggaacaga ttggcgctct tgattttcgc
     6241 cgttttatcg ttgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg
     6301 atttatcttt ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga
     6361 atcctggctt attaaccgta aaatcaaccg cccaacgcgt tgggccgggg gtgaagcgtc
     6421 gacagtgcca gcaccattac tggccgctcc gccacttatt ctcctgaagc ccggtacgac
     6481 aggcacgttg cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct
     6541 atttgagtta agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt
     6601 ggcgatgcgc tcggtattta aactgttctg gcgacccgaa ggttgccggg cgacccgctg
     6661 gaagcttatc aacaattacg ctggacacgg aagtcgcagg gtgtacaact cactaaccca
     6721 acgccttatt acattaacct gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgtg
     6781 ggagttgtgc cgcctaaaag ccagcgtcag acaagctggt gtcaggcaat agcaccgtgt
     6841 catgttgcct ggcgggcgat aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat
     6901 ctgccctgaa acaggttcgg aaaacgtttg cgtttttttt gccgcaggtc aattcccttt
     6961 tggtccgaac tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca
     7021 tgtactaccc cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt
     7081 ttacttttca gcaattacgc cgcattaccg gaacaccgtt tgaagcactg gtgcggcaga
     7141 aagtgcctgc aaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg
     7201 cagccggtct tgacaaagac ggggagtgca ttgatgcgtt aggcgcgatg ggatttggat
     7261 cgatagagat cggtaccgtc acgccacgtc cacagccagg taatgacaag ccgcgtctct
     7321 ttcgtctggt agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg
     7381 ataacctcgt agagaacgta aaaaaagccc attatgacgg cgtcctgggt attaacatcg
     7441 gcaaaaataa agatacgcca gtagagcagg gcaaagatga ctatctgatt tgtatggaaa
     7501 aaatctatgc ctatgcggga tatatcgcca tcaatatttc atcgccaaat accccaggat
     7561 tacgcacact gcaatatggt gaagcgctgg atgatctctt aactgcgatt aaaaataaac
     7621 aaaatgattt gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg
     7681 atctttctga agaagaattg atccaggttg ccgatagttt agttcgccat aatattgatg
     7741 gcgttattgc aaccaatacc acactcgatc gttctctggt tcagggaatg aaaaattgcg
     7801 atcaaaccgg tggcttaagt ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc
     7861 gcttgtcact ggaattaaac ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg
     7921 ttatcgctgc gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt
     7981 ttatttttaa aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc
     8041 gaattcctct ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat
     8101 tgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca
     8161 atggattgtg ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga
     8221 cgataactgg cgttggtatt acgatgaaga gcacgatcgt atgatgctcg atttagccaa
     8281 tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg ccttttctcc
     8341 cgcaggcttt tgcgttgacg acgccgcgct ctatttctct tttgaagaaa agtgccgcga
     8401 ttttaattta tctaaagaac aaaaagccga actggtgctg aatgcactgg tagcaattcg
     8461 ctatctcaaa ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt
     8521 gcccatgccg ggcgatgcgg cctgcgtttg gttgagcgac actaacgagc aagtgaattt
     8581 actggtcgtt gaatctggcg agaatgccgc gctatgcctg ctggcacaac cctgcgttgt
     8641 tattgcgggt cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa
     8701 accgcaggtt aatgttgaca gcttcagcct cgaacaggca gtctaacgcg ccaacttaag
     8761 tgcagtcttc ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt
     8821 tttcagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc
     8881 gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca acacctgttg
     8941 gttatttcca cgaaatgcct gtccctgcca gtcaatatct acattggcgt ccggttgttg
     9001 cgtgatgttg gcagtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgt
     9061 cagaatttcc acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg
     9121 accaaaatcg acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg
     9181 ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct
     9241 acaaggttta accacatcaa acaccacatc accaatgcga atcactttcc aactatcttc
     9301 ttcccaggct gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact
     9361 ggccggacaa cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata
     9421 gccatcagca aatgacaggg gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg
     9481 ccccacccag cgtaattgca cttcgcggga gaaaaatcca cttaaccatt tgttgatcgc
     9541 ttctggcgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt
     9601 ggcgaaatca gcaaaacgaa catatgcgct actgctatct ggtgcggtga gatgcaagcc
     9661 atcatgcacg ggtgaaggag taaatcgtac catctgggga aactggcgag cggtgataaa
     9721 cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatctgc
     9781 cagagcatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt
     9841 taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc
     9901 gcttattagc tataatgcgc aacaattttc ttagcgcctg tcccattagg ctattttatt
     9961 tgtcagtttg gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc
    10021 tccgcgctgt ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag
    10081 agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg
    10141 ttaaaaactg aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc
    10201 catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg
    10261 cgtattatgt tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt
    10321 gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc
    10381 agtggtttga atgacactat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg
    10441 atcgtcgatg ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg
    10501 gatatccgcg ttaacgtctg gctgcataaa gaaaccgcca gtatcgccct tgatctcagt
    10561 ggtgatggtt tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa
    10621 accctggcag ccgctattgt gatgcgatcc ggctggcagc caggaacacc gttgctcgat
    10681 ccgatgtgtg gttccggtac gttgctgatt gaagcggcga tgctggcgac cgatcgcgca
    10741 ccaggcttgc accgtgggcg ttggggcttt agcggctggg cgcagcatga tgaagctatc
    10801 tggcaggaag tgaaagcgga agcgcaaact cgcgcccgta aaggcctggc tgagtatagc
    10861 tctcattttt acggttcgga cagcgacgct cgggtgattc aacgtgcacg cactaacgcc
    10921 cgtcttgcgg ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc
    10981 aatccgctgc cgaaagagcc gtacggcaca gtgttaagca acccgccata cggggaacgt
    11041 ctggacagcg aaccggcgct gattgcgctg catagcctgc tgggtcggat catgaaaaac
    11101 cagtttggtg gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcttg
    11161 cagctgcgtg cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa
    11221 aattaccacg ttgccgaaag cactccagac agcaaaccgg cgatggtagc ggaagactac
    11281 gccaaccgcc tgcgtaagaa cctcaaaaaa ttcgagaagt gggctcgtca ggaagggatt
    11341 gaatgttacc gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat
    11401 gccgactggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg
    11461 cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac
    11521 aaactggtgc tgaaaacccg tgaacgtcag aagggcaaaa atcagtacca gaaactgggc
    11581 gagaagggcg agtttctgga agttaccgaa tataacgctc acttgtgggt gaacctgacg
    11641 gattatctcg atactggtct gttcctcgat caccgtatcg cccgtcgtat gctcggccag
    11701 atgagcaaag gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcat
    11761 gcgggattag gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggag
    11821 tgggcagaac gcaacctgcg tctgaatggc ctaaccgggc gtgcgcatcg cctgattcag
    11881 gccgattgcc tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat
    11941 ccgccaacct tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat
    12001 ctggcgctga tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg
    12061 aacaacaaac gtggcttccg tatggatctc gacggcctgg caaaactggg actgaaagca
    12121 caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac
    12181 tgctggctga ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg
    12241 catggctgtc gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata
    12301 acgaacgtgt ttgtctggtg ggccgcaacg gcgcaggcaa atcgacgtta atgaaaatcc
    12361 tcaaccgtga acaagggctg gatgacggtc gcattattta cgagcaggat ttgattgtag
    12421 cgcgtctgca acaggatccg ccgcgtaacg ttgagggtag tgtttatgat ttcgttgccg
    12481 aaggcattga agaacaagcg gaatacctga aacgctatca cgatatttcg cgcctggtga
    12541 tgaacgaccc gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc
    12601 accacaacct gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag
    12661 atcctaacgt tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac
    12721 gcgcgctggt gagcaatccg cgcgtattgc tgctggacga accgactaac cacctggata
    12781 tcgaaaccat cgactggctg gaagggttcc tcaaaacctt caatgggacg attattttca
    12841 tctcccacga ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg
    12901 gcaagctggt gacctatccg ggcaattacg atcagtacct gctggaaaaa gaagaagccc
    12961 tgcgcgtgga agaactacaa aatgccgagt ttgatcgcaa actggcgcag gaagaagtgt
    13021 ggattcgcca ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga
    13081 aagcgatgcg tcgcgagcgt ggcgaacgtc gcgaagtgat gggtaccgca aagatgcagg
    13141 tggaagaggc cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg
    13201 ttgacggaaa gcaactggtg aaagattttt ctgcccaggt tctacgtggc gacaaaattg
    13261 ccctgattgg gccgaatggc tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc
    13321 ttcaagcgga cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc
    13381 agcaccgcgc ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc
    13441 aagaggtgat ggttaacggc aagccacgcc acgttttggg ctatttgcag gacttcctgt
    13501 tccatccgaa acgggcgatg acgccagtac gtgcgctttc tggcggtgag cggaaccgct
    13561 tgctgctggc gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca
    13621 acgatcttga tgtcgaaacg ctggaactgc tggaagaact gatcgacagc taccagggca
    13681 cggtattgct ggtaagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga
    13741 tcttcgaagg cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc
    13801 agcaagagca gtatgtggcg ctcaaacagc ctgcggtgaa aaaaaacgaa gaacccgccg
    13861 cgccaaaagc agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg
    13921 aactggagca gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga
    13981 cgcaggtggc ggatgcttcc ttcttcagtc agccgcatga gcagacacaa aaagtgctcg
    14041 ccgatatggc tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg
    14101 aagcgttaaa aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa
    14161 cggtaaaatt gcctgatgaa ctgggtttat caggcatgaa aacaaacact ttgttagtca
    14221 tctgtaaaat agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc
    14281 acatcctgtg ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga
    14341 aagcggcatg tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc ccccggcagc
    14401 gtccgaccgc ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt
    14461 ttgtgaatat gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg
    14521 tgcttttttc tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg
    14581 ttcccgcgtt ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc
    14641 gtttaaaaga gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga
    14701 ttttcctcgc gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattggcg
    14761 taggcagcag ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt
    14821 gcgttgatcg tcgctggtta tgggacgata tcgccccgat gccagaactg cgccagccgc
    14881 taaaaccagg cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa
    14941 tccttcctgc tgatgaaccc gtgtgcccgc gctgtggtac taaagggtac gttcgacgta
    15001 gaaacagcct gcaatggaca ctcgcgctgc ttgttacgtc catcatgctg tatcttccgg
    15061 ctaatatttt gcccatcatg gtgacggatt tattaggctc gaagatgcca tcgacgattc
    15121 tcgctggggt catcctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc
    15181 tggccagtat tatggtgcca acgttaaaga tgatcgccat cgcgtggctg tgttgggatg
    15241 ccaaagggca tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt
    15301 ttgtaggccg ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc
    15361 gtatgggagg tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg
    15421 tcataatgac aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac
    15481 ctgaatcaga gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc
    15541 cagaaagtga agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg
    15601 gcctgggttc ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg
    15661 aatgcggaag gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc
    15721 gtggttgaaa gcgccacact ggctgatgat ttgacacacg ttgaaatcaa agcgcggctg
    15781 aattccggta tggaaaaatt gctgcataaa gataccgtct tttgggtggt gaaaccgcag
    15841 attggtcgag aagggattag cggtctggga accctgctgt ctggggttta tatcgaactg
    15901 cagccaggcg cgaaaggcag caaaatggat aaatacgatt tgctggactc gccaccgttg
    15961 gccccgcctg atgcgaaagg tatccgtgtg gttctcgata gcaaaaaagc cgggcagctc
    16021 tcgccaggag atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc
    16081 ttcgatacgc aaaaacgcaa tatcagttat caactgttca tcaatgcacc ttatgaccga
    16141 ctggtgacca gcaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca
    16201 gcgggaatgc gtgtggagat gggctcattg acaacgctgc tgagtggcgg tgtcagcttt
    16261 gatgtgccgg aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg
    16321 tatgatgatc agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg
    16381 ttctttaaag attcggtacg cggtctgcaa ccgggagctc cggtagaatt ccggggtatt
    16441 cgcctcggta ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac
    16501 gatgattacc gtattccggt gctgattcgt atcgagccag agcggctgaa aatgcagctt
    16561 ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga
    16621 tcgctgaaaa ccggaaacct ggtcactggc gcactgtatg ttgatctcga tttctatcca
    16681 aatacgcctg caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtt
    16741 agcggcggcc tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa
    16801 ctgccattga atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca
    16861 atgaaaaacc tgcaaacgac gctggatagc atgaacaaga tcctcgccag ccagtcgatg
    16921 cagcagttgc cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc
    16981 ttccagcccg gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag
    17041 gtgttgcgag aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta
    17101 tttgaagcga aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc
    17161 tagtgacgat tgcagcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact
    17221 attaccagtt acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgcc
    17281 tgttatgggt agagcaggtc gctgtacctg actatctggc ggggaatggt gtggtttatc
    17341 aaaccagtga tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc
    17401 aacagttgcg caacaccctg gtagccaacc tgagcacgca actgcccggc tgggtggttg
    17461 cctcccagcc tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg
    17521 gtcgctatga tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac
    17581 tgatcaaacg tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg
    17641 ttaaagtgct ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc
    17701 gtctacctta attataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg
    17761 tttttttgtt tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac
    17821 atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat
    17881 gattcgcggt attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc
    17941 agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg
    18001 cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc
    18061 agacgctgaa tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg
    18121 tagtaatggc ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct
    18181 tttgatactc tgtctgatta taatcagaag gcagacgtat cctggaacag accgactttc
    18241 aggtcgctgg cggtatagat cagacgacca tcaaccagca cttcgccatc cgccaggccc
    18301 ataatcagac gacggttaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct
    18361 gtcggcagta cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct
    18421 tcgccgccca gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg
    18481 catcccggca taaccggatc gccaataaag tggcatccga agaaccacag atccggattg
    18541 atatccagtt ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg
    18601 accacacggt ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac
    18661 agttcaccgc gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc
    18721 atgttctctg taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga
    18781 acaagtccga tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt
    18841 gacgtccttc ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg
    18901 tttggccttc gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt
    18961 ctactgccca gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac
    19021 ttaaatggcg aacgttagct gtggggataa tgacaccttg tttcccggtt aactcacgtt
    19081 gctggcaaat agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctgggcgc
    19141 gaccgaactg atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga
    19201 gggcgcttat cagggcgcag agttcagcca tcgaggcact atctccatca acttcactgt
    19261 atgactgctc aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg
    19321 acatcaggaa cgcttgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg
    19381 ctttgcgttc gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag
    19441 aaggttcgcc aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt
    19501 gcccgatgcg ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca
    19561 tacgttcagc gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct
    19621 cgccggagaa ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc
    19681 actgcgggct aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa
    19741 gtaccggcca ggcatccgct cccggggcag gtaagtgatt atgtctggcg gtaaatgtca
    19801 cccagcgaca ccactgggtt actgactccg catcgacaat ctgcagagta tcttcaaatt
    19861 cgctataaat agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt
    19921 cgcgttcgcc taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga
    19981 gagggcgcga ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca
    20041 gccgcatcca cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc
    20101 catttgcctg atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc
    20161 agccaaagag ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa
    20221 aattatcatc tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt
    20281 gaccgcccac cagttggcct gcatcgcttt gtagcgtacg cgcggcattg gcaataagat
    20341 tcagatactc agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat
    20401 gcagcaactg ctccagcgca aattgcagcc gcggttgagt atcactgaat aaaggatcgt
    20461 tttcgtcaat caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca
    20521 ggtcacgcca tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag
    20581 ccgcgattat accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg
    20641 catgggcgtt aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata
    20701 ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat
    20761 cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc
    20821 acccgttaca tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa
    20881 aatgaacccg tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat
    20941 cgcatgaagc aaaccattcg ggcaagacga aagcgccatt ttaatgcgga acaccagcat
    21001 acgcgcaaaa aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgcg
    21061 cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac
    21121 aaagagaaat acgcgaataa aatgtcttct ctgaagcagg atctgcaggc attgctgggt
    21181 aaggaataac tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc
    21241 ggggtttttc taccagacga gaacttaagc ttgcggctga gttacaacgt ctttgatacc
    21301 tttaacttcg atctctacgc gacgatccgg agccaggcag tcgatcagtg cagcacgctg
    21361 tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga
    21421 gatcttgtct gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg
    21481 ctcggacaga ccctggttgt aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac
    21541 tacggaaccg tctttcggat ccaggttgct cagctggctg tacagctgat ccagagcagc
    21601 ctgaccttcc ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt
    21661 gaagtgcttg gtctgtactt ccggtgccgg agctggagcc ggagcaacta ctggagctgc
    21721 ttcgccctga ccgaaacggt aggaaacacc caggctcagc atgccgttgt ccggacgagt
    21781 gccgatggtg tgtgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat
    21841 ttcaggagtg atcgcgtact caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg
    21901 gtttttacca taaacgttgg atttagtgtc tgcacgccat accataccac ccagacgagt
    21961 gtagatgtcc aggtcgtcag tgattgggta acccagttta gcggtcagtt gaacgccctg
    22021 agctttgtat gcaccgtttt caacgctgcc tttgtacggc atacgaccta accagtcgta
    22081 acccatttca aagccaacat acgggttaac ctggtaacca ccaaaagcac cagcgcccag
    22141 ttggttttca tgggtcgggc cattgttgtt gatgaaacca gtgtcatggt actgggacca
    22201 gcccagttta gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc
    22261 gaaaccagcc agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt
    22321 atcatccaaa atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg
    22381 gggatctgct caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga
    22441 tgtaaagtct acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa
    22501 aaaagtcttg tataaggtat gtttaatctt ttttgtcagc gacaatttac agaagagaat
    22561 cgcggaaacc gcttcagaca agcctccgca aggaaaatta gtcacgactg aaagcattgg
    22621 ctgggcgaca aaaaaagttc caggattaat cctaaattta cttaatgata caaattagag
    22681 tgaattttta gcccggaaag ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata
    22741 ataaacccca tagcgttacc ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca
    22801 gtcaaatcat ctgccaacca accgatcacc acactgtaat tgcccgtgcg taaagcgcga
    22861 accattgact ccacagtgtg gcaaggggag agctggctaa tctgcattac tttcgttaag
    22921 ggtagcccag atgcctgaac ccattcccga ctcagttttt gttgcggtgt taaccagagt
    22981 tgccagcgcg attgctgacc gagttgctgt aacaatggca acagtagaag ttgcgtcatc
    23041 atgggctgat cttcgcgata gacaacttca ctgataagcc cggctgtagt gttttccgta
    23101 gagacacgcg caattttact tgctgcggat gagaacgacg aagaacgatg tgcatagcct
    23161 gaagtgtaca taatcaatcc agcccctgtg agttactgta tggatgtaca gtacatccag
    23221 tgacaacaaa gatcaaccct attttcggaa agagcctcgc aaattttgtc gttggtgacg
    23281 ggaaaacata aattaatctt gccgcttaag aataagttgc ctattttcgt agttaacgga
    23341 tccgttaatg tgaatcattc ttttatgtta tgattttaaa aggaatttta tgaaaagcct
    23401 ctcctataag cggatctata aatcacaaga atacctggca acgttgggca caattgaata
    23461 ccgatcattg tttggcagtt acagcctgac cgttgacgac acggtgtttg cgatggtttc
    23521 tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca cagtactgtg taaaatatcc
    23581 gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt accctcaatt actatcgggt
    23641 tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt ctgtcgaaat attctctcga
    23701 tgcagcgctg aaagagaaaa gcacgcgcaa tacccgggaa agactgaaag atttgcccaa
    23761 tatgtctttt catctggaag cgattctcgg ggaggtgggg attaaggatg tacgggcgtt
    23821 acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac
    23881 agaaaagatt ctgtttatgc ttgaaggtgc cattatcggc attcatgaag ctgcgctccc
    23941 ggtggcacgc cgccaggagc ttgcagaatg ggctgactct cttacgccga aacaggagtt
    24001 tcctgcggaa cttgagtaat ctggcgttgc agacgaccaa tctcaggcag taatgcaatc
    24061 aataatccaa cttgttgtac gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc
    24121 tgcatccgct gtttcaggct agctaatgcc tcattgacgc gttcttcatc agcaggttga
    24181 tgatgtaacg cgtcatcaac atagcaaact gcgtcatcaa gaaacgccag aatttcagga
    24241 ttagttaact gctcccggtg agcaccgagg gctgagatat agctggtaaa cgtatggtta
    24301 aggcacagca accgaaacgc ggcttcgcga atttgcgggg taacgttcgg ctcgctggac
    24361 atatttgata ccaccgacgc cagctcagca tcacggttgt gtgcatcgcg gcgggcaata
    24421 cgatacgcca gacggttatc acgcccctga tggtattgct ccagtatggc atcgagatac
    24481 cgacagttgg cctctgtggc gcgttcgagc atgcgcggca gattgcgaaa ctgccagtca
    24541 ggccagatgt agctcactgc cgcccacgca atggcacaac caatcagcgt atcgattacg
    24601 cgaggtaacg ctacttcaaa accttcaccc agtaagttaa aacacagtag caccaaaagt
    24661 gtgatgaaca tcgttgcatg agcgtattgc acgttacgga aggcaaaaaa gagcacgccg
    24721 gtaataacca gcagcaccag ctgcccttcc agtgatggca caaaccacag cacaggaatg
    24781 ccaatggcga tacctaccag cgtaccaata atccttaact tcaggcggtg gcgcgtggcg
    24841 ttatagtttg gctggcagac aaacaaactt gtcagcaaga tccaataccc gtgatgcatt
    24901 ccggttatct gaatgatggc gtagccgaag cacaacacca gcgacattct taccgcatga
    24961 cggaagaggg cggattccgg cgtgaagtga cggctaagac gcagccagat atcactcaac
    25021 ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat tatggggtag tgcctgggcc
    25081 tgttctgatt caattgttgc cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat
    25141 cccagtgttt tgagtaaatc ggcgggtgcg ccgttatcgc gcatccgctc cagcgcagca
    25201 tcaatatgcg taaaagcgcg ctcaaaatgc ggatcatgtt gataaggctg acgcaacaaa
    25261 atacagcgtg acagttgctg gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg
    25321 aacagcacgt cgctgtggcg aaaatgttca cgcaatgttt gatactgaat atgagaagag
    25381 ctggcacgct cgtgaatatc ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc
    25441 cgttgaccac gatcgccacg taagcgggtc agcagcgaga gtttcgtctg attcaatgtc
    25501 gccatcagca gaccgttggc gagagccaaa tcgtacagcg gtgcctggct ttcatcttca
    25561 atatcaggat caaacatgcg cgacttgagc tcaagataac gcgccagttg ttcatagcaa
    25621 cgcgccaggt tgtcctgcag cgggcggacc gggaacagca gatgaccaat aagtgtcagg
    25681 acgttgtacc agacggcacc ggccagcaga tacatcggct gctgatacca gtgctcatac
    25741 agtgatgttc ccaacatagt gtaaatggcg atcagcaatg caccgaaggc aattgttgca
    25801 tagcgttgac ccagaccgcc gagcaaaatg aagccgctgg tagagagcgt taagccaatc
    25861 gcaaatagcc agggccaggg aaacagcaat tctactgagg ccgaggcgat aaaaaagcag
    25921 aacagcgtaa tgatgaggtt acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc
    25981 gccgctgcca ccatccccag cgttagcgga atcgtcagtt ttacatcacc cagccaccac
    26041 ggaaacgctg tggttccaca aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg
    26101 ttccaggtat agcgtttgag caaaggactt agcataaagg ccatattagt ggattattca
    26161 aaacgacgac gtgcattggc ttcacgcgca gcttgcgctg tttctaccga taccacgcga
    26221 cgaccaaccg gccatagtgc aatagcggca attttaaagt tcgcaatgcc gacaggaatg
    26281 ccaatgattg aaatacattg tgcgatgccc gttgcaatgt gcatcaggca taaccaccag
    26341 ccaaagaaaa tcagccagaa aatattcaat accgtaccgc cagtattcag cagcacattt
    26401 ttgccagccg ggttcagttc atcgacatga atagcttcat tgccataagg caccagagac
    26461 agtttagtga tctcccagca ggatcgtgtc agcggtaagg taaaaatcag cgcaatactg
    26521 accagagtcg ccaacagcca gcccagagtg gtggcaaatc cgccaagcac aaagttcaga
    26581 atgttcaaaa cggtacgcat aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa
    26641 ttgtaacgtt tttttgggct ggagcacgtt ttctctgacg gttacactga taagaaataa
    26701 tttcgtgtgg atctacagag tcatggaact gaaagcgaca acgcttggaa aacgtctggc
    26761 acagcaccct tacgatcggg cggtgatcct caatgccggg attaaagtct ccggcgatcg
    26821 ccacgaatac cttattcctt tcaatcaatt actggcgatt cactgtaagc gcggtctggt
    26881 atggggcgag ctggaatttg tactgccgga cgaaaaagtg gtgcgtctgc acggtaccga
    26941 atggggcgag acgcagcgtt tttaccatca tcttgatgct cactggcggc ggtggagtgg
    27001 cgagatgagc gaaattgcgt ctggtgtttt acgccagcaa ctggatttga ttgccacgcg
    27061 cactggagaa aataaatggc tgacgcgtga gcaaacctct ggcgttcagc aacaaatccg
    27121 ccaggctttg tcggcgttgc cgttgccggt taaccgactg gaagaattcg ataactgccg
    27181 tgaggcgtgg cgtaaatgtc aggcctggtt gaaagatatt gaaagcgctc ggttgcagca
    27241 taaccaggcg tataccgaag ccatgcttac cgagtatgcg gattttttcc gccaggtcga
    27301 gtcttcaccg ctgaatccgg cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt
    27361 agtgctggca ggtgcaggaa gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt
    27421 gctggcgcgt ggtgaagcgt cccctgagca aattttattg ctggcgtttg gtcgcaaagc
    27481 cgctgaagag atggacgagc gtattcgcga acggctacat accgaagaca ttaccgcacg
    27541 cacgtttcat gcgctggcgc tgcatattat tcagcagggc agcaaaaaag ttccgatagt
    27601 cagcaaactg gaaaatgata ccgctgcccg tcatgaactc tttattgctg agtggcgcaa
    27661 gcaatgcagc gaaaagaaag cgcaggcgaa gggctggcgg caatggctga cggaagaaat
    27721 gcagtggtca gtgccagaag gtaacttctg ggatgatgaa aaattacagc gtcgtcttgc
    27781 ctcacgcctc gatcgttggg taagtctgat gcggatgcac ggtggtgcac aggcagaaat
    27841 gattgccagt gcacccgaag agattcgcga tctgttcagt aaacgtatca agttgatggc
    27901 cccgttatta aaagcctgga aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg
    27961 ccttattcat caggcgattg tgattctgga gaaaggtcgc tttatcagcc cgtggaagca
    28021 tattctggtt gatgaatttc aggatatctc gccgcagcgg gcagcgttgt tagcggcatt
    28081 acgcaagcaa aacagtcaga cgacgttgtt cgccgttggt gatgactggc aggcgattta
    28141 ccgattcagc ggtgcgcaaa tgtcgctcac caccgctttc catgaaaact ttggtgaagg
    28201 cgaacgctgt gatttagaca cgacttaccg ttttaacagt cgtatcggtg aggtggcaaa
    28261 ccggtttatt cagcagaacc caggccagct gaaaaagccg ctaaacagct taaccaatgg
    28321 agacaaaaaa gccgtcacgt tattggatga gagtcaactt gacgctttgc tggataagct
    28381 ctctggttat gccaaaccgg aagagcgcat tctgatcctg gcgcgttacc atcacatgag
    28441 gcctgccagc ctggaaaaag cggcaacacg ctggccgaag ttgcaaatcg actttatgac
    28501 cattcatgcc agcaaagggc aacaggcgga ttacgtcctc atcgttggct tgcaggaggg
    28561 aagtgatggt tttccggctg cggcgcggga gtcgattatg gaagaggcgc tactgccacc
    28621 ggttgaggat ttcccggacg ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc
    28681 acgtcatcgg gtatgggcac tgtttaacaa agagaatccc tctccctttg tggaaatact
    28741 gaaaaatctg gatgtgccgg tggcgagaaa accgtaagaa acaggtggcg tttgccacct
    28801 gtgcaatatt acttcagacg gtccgcgaga taacgctgat aatcggggat cagaatatcg
    28861 accgcgtcgt tgaaatgcgg cgactggata atgaagtctg ccgttgccac gttggtggcg
    28921 accggaatgt tccataccgt cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc
    28981 acggcattta gtggatccca gaagaaaatc aatacatcaa ttttcccttc tgagatcaat
    29041 gcgccaacct gctggtcacc ccccattggg ccactcaaca tcgcgttgac gttcatgccg
    29101 gtcgcgcggg aaattaagtt accggtagtg cctgttgcat acagtacgtg ttgttccagt
    29161 aacggttgat gccgttccac ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc
    29221 agcgcaatat gtttccgcgc aggtaaagtg cgagtcgtca gttccataat gtacatccgt
    29281 agttaacttt cctacagatt actgtaagca cttatcgctg caagataaag accgaaaaag
    29341 cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt ttccgcccat tgcaggaaag
    29401 tatttcgcgt ttgtgggtca gccagtttaa accaatactt cagccgttgt tctgtgagca
    29461 cctgagactg cggtggaata gcatccagct cggaaacacc cgaaagcagc gtactgtcat
    29521 ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt
    29581 caacctcgac ctcataatca atcgtttttg ccgttgaggt aatggcgaga atatccagct
    29641 ttaccggaat cggcgtcgca tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat
    29701 ggttagcttc ccgctcattc tccaggcgag gcaggcgaaa attgacctgg ttgatgagct
    29761 gggtattaaa actgaccacc agcggtgggg agatatacag ccgttcttca ctattggaaa
    29821 gatgaatcgt cttctcaacg cgaaacacta actgatgcgg tccattatcc agttcaatgc
    29881 tgtccgcacc gcgcagcaga gagctggaaa ccttcttgcc gtcgagtacc agaagatcga
    29941 catcggttga aagccgcagc gtggtggcaa aaacggatac cggcaaacat aatgcaatca
    30001 aggtggtcac gatgccggtt ttcataggta gctcctgtca aaagaccgcc cgaataataa
    30061 tgtgtcaaaa tttttcttta atcatactta ctaacatata gacatatttc ccgcttttgc
    30121 tctcattcat tcgtattagc tgcatggttg gcatgtcggc tttgtcgtac actttgtgaa
    30181 acagtctgga ggaaataatg aaagaaaccg atattgcagg cattttaacg tccactcaca
    30241 ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc cagctatcgc gtgatgaaat
    30301 atctactcga ccaggggtat cacgtcattc cggtttcgcc aaaagttgcc ggcaaaacgc
    30361 tgctgggcca gcaggggtac ggcacgctgg cagacgttcc ggaaaaagtc gatatggtcg
    30421 atgttttccg taattcagaa gcggcgtggg gcgtagcgca ggaagctatc gccattggcg
    30481 cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca agcggcagta ctggcacggg
    30541 atgccgggtt gaatgtggtg atggatcgct gcccggctat tgagatccct cgcctggacc
    30601 tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg ggatctgttc agggattagt
    30661 tacgcagacg cggggcctgg agttgtttgc ggatggtctg cgccagctca tccatggatg
    30721 gctgttcagg atgttcatcc tgcaattcgc tacttagctg ggcttcagcc aggtaggtat
    30781 gaaccggtag gccgttatcg tcctccatca ccacgtgata ccacggagcg gcgcgaagct
    30841 cgtcattcac cgccagctca tcaggcgacg gttcagagag cgaataaacc gggtcgatat
    30901 ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg ataccgaatt
    30961 tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg gtaaaattcc
    31021 acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct tccggatagg
    31081 tagcgatcac cggatgatcg gccgcctgac ggaactgctc tataaattgt acatcacgac
    31141 cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc atcagaccgg
    31201 aacaggagaa agtcaggaga ataccgcctt cattcagcag ctgaatcgcc agcatgttga
    31261 tatctttata gccacgacac gcgcccatca actggctttt attctcaaca aacttcggcg
    31321 ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataggtacgc agcaatttaa
    31381 agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagc tcaacgttct
    31441 gccgtgcaat atccagtgct tcctgggagg tatcaacgct gacaacctgg ctgcaaccgc
    31501 ccatcagtgc cgatacggcg aaaccaccgg tataggagaa acagttcagt acgcgtttat
    31561 tttcaacgta gcggcgggta gccaggcggc tgtctcgctg gtccaggtag tagcccgttt
    31621 tgtgtccgtg ctgtatgtcc accagcagct tcatgccatg ttcttcaatc ggcagcaggg
    31681 caggcggcaa ctcaccggtg acgaggccct gggtcagctc catcccttct tttttacgta
    31741 ccgcaacatc gctgcgatcg taaatcgcac attccgggta cagcgtttgc agggcactaa
    31801 ttaatgccgc gcgctgatat tctgcgccag cactcagcag ttgcagcacc agaaaattac
    31861 cgaaacgatc gatagtaata cccggcaggc catcagattc tccggcgatt aaacgatagc
    31921 tgtcgaggcc atctttttgc gccagccagt cacgccattt ttgtgcttgt tgcaaacggc
    31981 gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa cgtccagacg cgcgcccgga
    32041 tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga tcaacaatat
    32101 cgatggtttc accgaggctg gctttacctt ccatacgggc aacggccccg gaaaagaccc
    32161 acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa cgtacactca
    32221 taatttactt ttctgtcgat gcaaagaaat gggcatcatt gtccggagtt ttccatggat
    32281 ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc ataattgcct
    32341 gggtttacgg gcgagttcag ggcgtaggat ttcgctacac cacacagtac gaagcgaaaa
    32401 gactgggctt aaccgggtac gccaaaaatc ttgacgatgg cagcgtggaa gtggttgcct
    32461 gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt aaagagtggc ggcccgcgtt
    32521 ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggggaa ttaaccgatt
    32581 ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc agcaattttg gtggcttgct
    32641 tagccggacc tttcggaaac agtcggtata aatagcggct gttacctttt tcttcgccaa
    32701 atttattcgc catcgctttt accagcatac gaatcgccgg agaagtattg aattccagat
    32761 agaaatcacg cacaaaacgc accacttccc agtgttctgg cgacagcgaa atcccttcgt
    32821 tctctgcaat caccactgcc agcggctcac tccactggct gctttctttg agatagcctt
    32881 cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact accttaatca
    32941 gactgccggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat gaaagtgtta
    33001 gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg acgaagatgt
    33061 tgtacagcga aacatacagg ctaaccgtgg cacgaatata gttcgtttca ccgccatgaa
    33121 tgatgttgct ggtttcaaac aaaatagcgc cagaggagat caaaatgaag accgcgctga
    33181 tcgccagatg cagagcaggc agctgcagga agatattagc aaccatacca atcagcacca
    33241 ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg gtggtcagca
    33301 catatgcaga gcagcagaag aacactaacg ccgttccgcc cagtgccata gcgattacgt
    33361 cacccattcc ggcagacaga taggtattca gaattggtcc gaggatataa cccagaaaac
    33421 cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccatttta taggtcagga
    33481 acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc agcatcagca
    33541 ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc agaaaatagg
    33601 tattacgcag cactttatgg gtgctaagca gtgatgtacg gtcatgtgaa gaactaacaa
    33661 tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca taatgataat
    33721 gcgtaagggc acccagtagt tttacccatc tttacgcatt tgatctggaa caggtttaac
    33781 agcggattat caggtcatta agcaaatata acgccctgag aatttcggca ggcaaaagaa
    33841 aaaggggtta gcatttagct aaccccttat cttatctggc ggaagcgcag agattcgaac
    33901 tctggaaccc tttcgggtcg ccggttttca agaccgaaga aaaacaaata aaaatcagtg
    33961 taatacgtga aattgctgaa aagttaatca aatttaacac caagaaaaat caaggtatta
    34021 ggaagttgct catagctagt ataccaagtc tttttgccag aaatttttct ggattgtcta
    34081 cttgctaacc acgagcggca aatccagtgt tggtgtgatt tttgttttac gatcgtaaat
    34141 caacacctga ttttctgttt tatgcccgct gaaaatttgt ttgtcgcgac tgctgccttc
    34201 gtaatctgag atccccttgg cttttatgtc gtgaaaattg cacccgaacg gaacgccaac
    34261 tttttgctcg gctgcgcgtt tagcctgatt ccaccagtta ttcagcgttt tagctatgac
    34321 tttcccgcct ttggttgtat tgatcacata ttcgcatgta caggaagata catttcgggc
    34381 taactggatc gctgtacgta atcgcggtga ccattccttg atttgtttgg tgccggtttt
    34441 gttttgctca atgtagatcc ctttatccat aatatcctgc catttcaact caagcacatc
    34501 accgagtctt gccgcacaga gataggaaat ctccattgca atgcgtaact gtggaattgc
    34561 ttccgcatat atcgccgcat actgttcatc ggtgatgtaa acagtgcggg ctttaagaga
    34621 gaattttctg actcctttgc atgggttatt cttcacatac ccacgctcat atccccaccc
    34681 gtatactcga ctcaggcttg ccagttcatg atttgcctgg gtcttgctct caagccctcg
    34741 tttatccatg aaaattctta cctgctcagt tttgacatta tcagcaagca cttttccgaa
    34801 taccatcagc aacgccttct gatgttgtcg ataatctttt tgggttcggg gggccagttc
    34861 tgtaaatgca ggggagtcca taaacatgtg ccacaattta gctacggtca ttatgttgtg
    34921 gagttttgct ttttccagtt cataattttg ccagacttta gctacgctgg ttttccgcac
    34981 tcttcctagc cctatacttc ttgtacttcc ttcgggtttc cacacgtaac tgtaaccatt
    35041 cgatctaacc cgcggtggca gtacattatc ttttttattt tttcttggtc ttcccattgt
    35101 tcagcgcctc aaaatcgggt tcagcagaaa ccagttcaga tgtttttggc atcgttgtca
    35161 gaccgtgtgg aatatccctg cggagaacta tgggttcgtt tttagggccg attacaaatg
    35221 ggatgccgtg cagtcttaac tggtgttgct gttttgtgta tcgctcgtat ttcgtgatct
    35281 ctttaatctc tgctggagat agagttaatt cgtacatgtg gtcacgtgcc tttacagcat
    35341 gaccgcggcc actatagctg gtgggcggcg atatgggttg aacatcaaaa atcagcctga
    35401 ctcgggagca gtttttgcca gatggctgaa acgtatttcg cctggtaacg
//
LOCUS       DAZFKY010000026        35205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFKY010000026 DAZFKY010000000
VERSION     DAZFKY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(54..704)
                     /locus_tag="ACHKCL_001170"
                     /pseudo
     CDS             complement(54..704)
                     /locus_tag="ACHKCL_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(967..1257)
                     /gene="ilvN"
                     /locus_tag="ACHKCL_001171"
     CDS             complement(967..1257)
                     /gene="ilvN"
                     /locus_tag="ACHKCL_001171"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5762182.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1261..2949)
                     /gene="ilvB"
                     /locus_tag="ACHKCL_001172"
     CDS             complement(1261..2949)
                     /gene="ilvB"
                     /locus_tag="ACHKCL_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5762183.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3055..3153)
                     /gene="ivbL"
                     /locus_tag="ACHKCL_001173"
     CDS             complement(3055..3153)
                     /gene="ivbL"
                     /locus_tag="ACHKCL_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5762184.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3718..3807
                     /gene="tisB"
                     /locus_tag="ACHKCL_001174"
     CDS             3718..3807
                     /gene="tisB"
                     /locus_tag="ACHKCL_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO5762185.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(3931..4005)
                     /gene="ysdE"
                     /locus_tag="ACHKCL_001175"
     CDS             complement(3931..4005)
                     /gene="ysdE"
                     /locus_tag="ACHKCL_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5762186.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            4087..5271
                     /gene="emrD"
                     /locus_tag="ACHKCL_001176"
     CDS             4087..5271
                     /gene="emrD"
                     /locus_tag="ACHKCL_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5762187.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(5279..5776)
                     /gene="yidF"
                     /locus_tag="ACHKCL_001177"
     CDS             complement(5279..5776)
                     /gene="yidF"
                     /locus_tag="ACHKCL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5762188.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5773..6135)
                     /gene="yidG"
                     /locus_tag="ACHKCL_001178"
     CDS             complement(5773..6135)
                     /gene="yidG"
                     /locus_tag="ACHKCL_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5762189.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(6125..6472)
                     /gene="yidH"
                     /locus_tag="ACHKCL_001179"
     CDS             complement(6125..6472)
                     /gene="yidH"
                     /locus_tag="ACHKCL_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5762190.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            6581..7030
                     /gene="yidI"
                     /locus_tag="ACHKCL_001180"
     CDS             6581..7030
                     /gene="yidI"
                     /locus_tag="ACHKCL_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO5762191.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(7077..8570)
                     /gene="yidJ"
                     /locus_tag="ACHKCL_001181"
     CDS             complement(7077..8570)
                     /gene="yidJ"
                     /locus_tag="ACHKCL_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5762192.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(8567..10282)
                     /gene="yidK"
                     /locus_tag="ACHKCL_001182"
     CDS             complement(8567..10282)
                     /gene="yidK"
                     /locus_tag="ACHKCL_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5762193.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            10449..11342
                     /gene="yidL"
                     /locus_tag="ACHKCL_001183"
     CDS             10449..11342
                     /gene="yidL"
                     /locus_tag="ACHKCL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5762194.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(11339..12661)
                     /locus_tag="ACHKCL_001184"
     CDS             complement(11339..12661)
                     /locus_tag="ACHKCL_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO5762195.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(12661..14277)
                     /locus_tag="ACHKCL_001185"
     CDS             complement(12661..14277)
                     /locus_tag="ACHKCL_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5762196.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            14573..15289
                     /gene="yidP"
                     /locus_tag="ACHKCL_001186"
     CDS             14573..15289
                     /gene="yidP"
                     /locus_tag="ACHKCL_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5762197.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(15286..16947)
                     /gene="yidE"
                     /locus_tag="ACHKCL_001187"
     CDS             complement(15286..16947)
                     /gene="yidE"
                     /locus_tag="ACHKCL_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5762198.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17143..17571)
                     /gene="ibpB"
                     /locus_tag="ACHKCL_001188"
     CDS             complement(17143..17571)
                     /gene="ibpB"
                     /locus_tag="ACHKCL_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5762199.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(17683..18096)
                     /gene="ibpA"
                     /locus_tag="ACHKCL_001189"
     CDS             complement(17683..18096)
                     /gene="ibpA"
                     /locus_tag="ACHKCL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5762200.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18090..18162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            18402..18734
                     /gene="yidQ"
                     /locus_tag="ACHKCL_001190"
     CDS             18402..18734
                     /gene="yidQ"
                     /locus_tag="ACHKCL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5762201.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(18736..19950)
                     /gene="yidR"
                     /locus_tag="ACHKCL_001191"
     CDS             complement(18736..19950)
                     /gene="yidR"
                     /locus_tag="ACHKCL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5762202.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWPVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            20051..21115
                     /gene="cbrA"
                     /locus_tag="ACHKCL_001192"
     CDS             20051..21115
                     /gene="cbrA"
                     /locus_tag="ACHKCL_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5762203.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(21112..21225)
                     /locus_tag="ACHKCL_001193"
                     /pseudo
     CDS             complement(21112..21225)
                     /locus_tag="ACHKCL_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21361..22017
                     /gene="yidX"
                     /locus_tag="ACHKCL_001194"
     CDS             21361..22017
                     /gene="yidX"
                     /locus_tag="ACHKCL_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO5762204.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(22063..22875)
                     /gene="yidA"
                     /locus_tag="ACHKCL_001195"
     CDS             complement(22063..22875)
                     /gene="yidA"
                     /locus_tag="ACHKCL_001195"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5762205.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(22990..23388)
                     /gene="yidB"
                     /locus_tag="ACHKCL_001196"
     CDS             complement(22990..23388)
                     /gene="yidB"
                     /locus_tag="ACHKCL_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5762206.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            23506..23604
                     /locus_tag="ACHKCL_001197"
                     /pseudo
     CDS             23506..23604
                     /locus_tag="ACHKCL_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23628..26042)
                     /gene="gyrB"
                     /locus_tag="ACHKCL_001198"
     CDS             complement(23628..26042)
                     /gene="gyrB"
                     /locus_tag="ACHKCL_001198"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5762207.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(26071..27144)
                     /gene="recF"
                     /locus_tag="ACHKCL_001199"
     CDS             complement(26071..27144)
                     /gene="recF"
                     /locus_tag="ACHKCL_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5762208.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27144..28244)
                     /gene="dnaN"
                     /locus_tag="ACHKCL_001200"
     CDS             complement(27144..28244)
                     /gene="dnaN"
                     /locus_tag="ACHKCL_001200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5762209.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28249..29652)
                     /gene="dnaA"
                     /locus_tag="ACHKCL_001201"
     CDS             complement(28249..29652)
                     /gene="dnaA"
                     /locus_tag="ACHKCL_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5762210.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            29949..30029
                     /locus_tag="ACHKCL_001202"
     CDS             29949..30029
                     /locus_tag="ACHKCL_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762211.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            30259..30399
                     /gene="rpmH"
                     /locus_tag="ACHKCL_001203"
     CDS             30259..30399
                     /gene="rpmH"
                     /locus_tag="ACHKCL_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5762212.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            30416..30775
                     /gene="rnpA"
                     /locus_tag="ACHKCL_001204"
     CDS             30416..30775
                     /gene="rnpA"
                     /locus_tag="ACHKCL_001204"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5762213.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            30739..30996
                     /gene="yidD"
                     /locus_tag="ACHKCL_001205"
     CDS             30739..30996
                     /gene="yidD"
                     /locus_tag="ACHKCL_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5762214.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            30999..32645
                     /gene="yidC"
                     /locus_tag="ACHKCL_001206"
     CDS             30999..32645
                     /gene="yidC"
                     /locus_tag="ACHKCL_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5762215.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            32751..34115
                     /gene="mnmE"
                     /locus_tag="ACHKCL_001207"
     CDS             32751..34115
                     /gene="mnmE"
                     /locus_tag="ACHKCL_001207"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5762216.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(34265..35205)
                     /locus_tag="ACHKCL_001208"
                     /pseudo
     CDS             complement(34265..35205)
                     /locus_tag="ACHKCL_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
BASE COUNT         8535 a         9259 c         8812 g         8599 t
ORIGIN      
        1 cagcaagtta ttttagtagc acccttttgt tgactttgtt cttttatagt tcaatgagca
       61 tccataatca tggaatcaaa aaaaacatca gttcttttaa aactttcctg aacaggtaca
      121 tacccttcat cttttttaat tcgcagtttt aataaatcat atatattact taaaattcct
      181 gcaggaattt cgtaattaaa atccgtattc ttcataacaa tactattaac taatttcttc
      241 ctcaaaaccg tgtcagcgga atgtgtttct aacgcagaag ttttttcaga caacgattta
      301 ataatagtcg ctacagccac tcctgaatat ggaatttctt ttcgtgaagg tttatctctg
      361 ccaaccagtg taatattaaa agacttatca cctgtttgtt tgatttcgat atattcatcc
      421 tctccagttg ccgccaccca ggtacgatta ccattatttt ccgtgcctgg catagggtgg
      481 aaatagtcaa gaatatcggt gttaatactc cgagaaccgg acatgatgtt tagtattaag
      541 cgctttattt cctgcaacat ccattttgcg aaattactgc caggtgaata ttttttctcg
      601 ttatttttaa tatcaagctc agcagcgaca tttctatttt tatccaaaaa ggtggaaggg
      661 cagagatttg ccatcattat tccgctgttt ataggacttg ccataatata cctcacgatg
      721 aaccattaat taatagaatg ctggtcatga ccactggtat agacagcaat cagaatttaa
      781 caccatcgct gaaaatttta gccctaactg tcccccccag gttaccggtg ctgcgactta
      841 aattaacgtc ctgggctaat aaattgtgcc tgagatggtc tctggcacaa tttcacccta
      901 tatatctgca cggatctatc aaaaatcgct catcagtaac tcccccgagc ggtttaatgt
      961 cgctccttac tgaaaaaaca ctgcaatctt gttaaacatc gtcggatcgg actgattacg
     1021 ctgcactttc accacatctt ccagcttatc gatttggctt atcatctgct ccagacgctg
     1081 gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg gcagacaaag
     1141 aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg tcattacgcc
     1201 cggatggttg cgaacggtga gttccagaat cacgttgtca tgagttgtgt tttgcatggc
     1261 ttattccccc accatttctg tattcgccgc acctggcggc accatcgggt acactttttc
     1321 ttcggcatca atgcgcacat ggatcagcgc cggaccaggg cgattgatga tttcctgcaa
     1381 tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga atccggcggc
     1441 aatctgcata aagttgattt ttcccggata ggtagcggca aaaacgccct gcttgtagaa
     1501 cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa tgattttaac
     1561 atccagctgg ttttcgctgg cggtcgccat ctcctgaata ttcatcatca ggctgccatc
     1621 gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa tcgccgcagg
     1681 caggccaaaa cccatcgtgc ccagcccgcc ggaggtcagc cactggcgtg ggcgattgag
     1741 cggataagcc tgcgccgtcc acatctgatg ctgacccaca tcggtggtga taatcgcatt
     1801 gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaacg gatcgcacgc
     1861 tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt gccactctgc
     1921 acgcggttgc gcttccacct gcgggatcag ctgcgccagc acgtcatcaa catccgcctg
     1981 aatcgctacg tgcggctgct tgattttacc cagctctgcg cggtcgatat cgacatgaat
     2041 aattttggca ttcgggcaga actgctcggt tttgccaatc gcccggtcat caaaacgcgc
     2101 accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtgctgc gcacaccgtg
     2161 catccccaac atacccagcg acaacggatg cgcttttggc aacatgccca gcgccattaa
     2221 agtcattgtg gtaggcagct gcgctttctc cgccagttca cgcactctcg caggcgcatt
     2281 gatcacacca ccgcccagat aaagcaccgg gcgtttggca gcattaatca ttgccgctgc
     2341 gtcacgaatg ctttcttcgc taaaggcggg cgcgacggct ttttctgcca ccgcgggctg
     2401 tgcttcaatc tcaaaaacgg ccgtttgcac atccttagga atgtctatcc acaccgggcc
     2461 agggcggcct gattgcgcaa tacggaaggc atcgctcatg acctgcggga gttcttcgat
     2521 atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg tgtcgacttc
     2581 ctggaaggcg tcagtgccga tcattgaggc gggaacctga ccagtgatgc aaatcagcgg
     2641 gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgccc ccggtccgct
     2701 acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag cgataaaccc
     2761 cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc ttaaggcatc
     2821 gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc cctgctgttc
     2881 caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg atgtgcccga
     2941 acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc ttaaacgaaa
     3001 aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca gtccctacgg
     3061 cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg gcgcagttgg
     3121 tagtagtttt gcgttgagca tggaagtagc cattggggac cttgttggtt ttgtgtttaa
     3181 caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc agcaagggaa
     3241 aattgagggg ttgatcacgt tttgtactgt actgcaggta acaaaaaacc ccgccggagc
     3301 gaggtttcgt cagccgcccg cggctggtaa ccgcaaagca cactgtatta tgtcaacaca
     3361 gaaagtatac gtgttccgcg caaaacgcgc aatttcggca cgaattttga cgtatttagt
     3421 gcatagttga gtatcgatca cagtttgcgt tttgtccaaa tattactgtt tatttataca
     3481 gcaagcttct ataatatcac tgtacgcaat gtgttatgcg ggggccgcat cgttacccgg
     3541 cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg cgtgagcaca
     3601 ctgtgttatg tcaacgagca caacgtttct ccttgagata ccgcgtgcac aacagctggc
     3661 aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga gacgcgtatg
     3721 agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact gcaactgctt
     3781 gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcactaaag gggagcgggt
     3841 aaccgctccc attttatatt tagcgtgtgg gttggtgtcg gatgcgatgc tgacgcatct
     3901 tatccgacct accatccctc ccggcaacat ttattgccgc ttttgtttac atattctgcc
     3961 gctaaacaat tccccattcc tggcgtatat ctggctaaca tttatcaata tgatagattc
     4021 ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat aaaagcatcc
     4081 gtgataatga aaaggcatag aaacatcaat ttgttattga tgttggtatt actcgtggcc
     4141 gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc gcgcgatctc
     4201 aacgttcgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac ttacggtgtc
     4261 tcacagctgt tttatggccc gatttccgac cgtgtgggtc gccgaccggt gatcctcgtc
     4321 ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag tttgacagta
     4381 ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt
     4441 actttgccgc gtgatttata tgaacggaca cagttgcgcc acgctaacag cctgttaaac
     4501 atgggaattc ttgtcagtcc gttgctcgca ccgctaatcg gcggtctgct ggatacgatg
     4561 tggaactggc gcgcctgtta tctctttttg ttggtacttt gtgccggtgt gaccttcagt
     4621 atggcccgct ggatgccgga aacgcgtccg gtcgacgcac cgcgcacgcg cctgcttacc
     4681 agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat gctgattggc
     4741 ggtctggccg ggattgccgc ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta
     4801 gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc ggcatttttt
     4861 ggggcatggt ttgccggacg tcctaataaa cgcttctcaa cgttgatgtg gcagtcggtt
     4921 atctgctgcc tgctggctgg cttactgatg tggatccccg actggtttgg cgtgatgaat
     4981 gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat gctgtttccg
     5041 ctggcgacca gcggcgcgat ggagccgttc cctttcctgg cgggcacggc tggcgcgctg
     5101 gtcggcggtc tacaaaacat tggttccggc gtgctggcgt cgctctctgc gatgttgccg
     5161 caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt gatcgtgctg
     5221 tgctggctac cgctggcgac gcggatgtcg catcaggggc agcccgttta agctcacgtc
     5281 accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga tcacgcatca
     5341 cccgcatatg cggcgcagag taagaataaa acgcctgata gcccgcacaa agcacgctgt
     5401 ctggcgtctc ctcccgagcg tgcggacaca gcagatgcca gtcgcacgcc tggcagccac
     5461 ggcgcggttg cgggttttcg ctgccacacc atttttgtaa tgtggcgtca aaaagctcaa
     5521 tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt aaaaatttcc
     5581 cccacagctc tgctgtcacc gaagcggccg tcatatcgcc ccggtcatca aattcaagat
     5641 ggggaataag ctgtacggag cggatgcccc gttgtttgaa attgtgataa aaatcggcag
     5701 gttgcagagc atccttgtac tctacaacca gtttatgcct gtcctcttca gcatcaatga
     5761 cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta tatgcgttac
     5821 agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt cacggactac
     5881 gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac gagtgtagtg
     5941 ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata acatgcccgc
     6001 ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca gcatggtacg
     6061 aaaccacgcc agtgatgtac gctccggctg cagccctggg tcggcaatgc gtctggcttt
     6121 gcggctatcc ggcatacaac accagtccca taacgatcac tgctacgacc atcaatatta
     6181 agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt
     6241 cattgcgtaa ccagcgcaag tagccataca tcgctaaccc gccggaaaac agacacaaca
     6301 acaacgccag cagttcgcga atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc
     6361 caacccccgc cgccagaaat cctagtgcag tacggatcca cgccagaaaa gtacgctcat
     6421 tagccagcga gaagcggtaa tcaggcgctt ctccgaggcg ggaaattttc attgttgctc
     6481 ctttgcaatt tcactaacgc tctaccttta gcggtttttt ttaagaattt cgtccgcccg
     6541 tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca tagcgcaaaa
     6601 taaaatcagt tcattgggca tgctattcgg tgccatcgct ttaatgatgg ggattatcca
     6661 cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg tggcggacaa
     6721 aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa ttaccaccgt
     6781 ggagagaaaa gaggacgtgg atgtcgataa aatcctcgat caaagcggta tcgcactggc
     6841 cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc gctggggcat
     6901 tcgcggcgcg cgggtttttg gcggcgggac gctggctttc cataccttgc tttttggtat
     6961 cggcatcgtg tgcagcattt tgctgatatt ccttatcttt tccttcctga ctggcggttc
     7021 actggtttaa gcagcctgaa taatgcccgc cgggtgttca tcagcgggca ttatcgtcag
     7081 aacttctgtt ttttctcttc caccttcacc ccttgtgtcg gcaggccggt gtcatagtcg
     7141 cgtaccactg gcgaataacc atcttgtggg cgtggacgaa atgcccccat ccagcgcggc
     7201 tgtgcatctt tacgccacgg acgcaggctc cattggtaac tgcggaacgg atcgcgaatt
     7261 ttgtccatat aatccaataa ggcatcatgc attttgctgc gaacgtcggc aaaatgggca
     7321 tcatcgatca ggttatgcat ttcatttgga tcattacggc gatcgtaaag ttcatcactg
     7381 gtgaagaggt tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa
     7441 ccgccaaagc tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc
     7501 tctttcactg caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc
     7561 gccatcattg tcggcagtaa atcgatatga ctgactggcg tatcgacctg tcgccgctcc
     7621 ccttgcggcg aacggatgat cagcggaatg cgggtgatgt cgtcatacat cgccgcccct
     7681 ttactgatca gcttatgtgc gcccatcatt tcgccgtgat cggaggtata aataacccac
     7741 gtattttcac gttgctctgg ctttaaggca ttgatgaccc gtccgatttg gtcatcaaca
     7801 aagtcattac aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat
     7861 ggcatcgcct gcgcccataa gcggtgatgt tctggtttgt tcgccaggtc atcctgtgct
     7921 ttctcgccca gctcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg
     7981 aacgggtgat gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcgcg
     8041 ggctgttgca gaaaatccac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg
     8101 tcaatatggt tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctaatc
     8161 tctttttccg tcagttcgct aagatagttc gccccatcga accagtaatc agcgtcccac
     8221 tccggcggac actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg
     8281 taacaggtgt gatagccggc atccttaaag tagcgcccca tagtggagat gtttttaccc
     8341 ggcgcgacgt tgttggtcca cggcccggac tggttagcgt agataccggt aaacagcccg
     8401 gcgcgcgccg gtgtacaaac cggtgaacag gtgtaggcgg aattaaagcg aataccttcc
     8461 gccgccagac tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata
     8521 ttgggggcct gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct
     8581 tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg ccgccagctg tcaaaaatca
     8641 ggtagacaat cactacggcg gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt
     8701 agccgccgaa ttcagccagc ccggcataga cgccaatcat cgcgaacagg atgccaattg
     8761 acgcgatctt gacgtttttc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga
     8821 acggcgtggc gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga
     8881 acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga
     8941 tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccggg atgcgcagga
     9001 agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt
     9061 tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgctacc agcaccgaaa
     9121 cgatagcgat aaagaaacca aatttgcgcc cgacggtgac cagctgctgc ggctcggcat
     9181 tctggttaat gatgcgacgg taaataccca tactgaataa cgtactggcg ctattcagaa
     9241 agccgttgaa ggtactgatc actgcaccaa ataacaccgc gccgaagaaa cccaccagtg
     9301 gcactggcag aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtagatcct
     9361 gatacagatg aaacgcaatc aaccctggca gcaccagtac cagcgggtcg agcattttca
     9421 gcaccgccgt taacagcgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc
     9481 gttgcacgat gccctgattt gtacaccagt aaaaggtgtt caccagaatt aaaccggtaa
     9541 atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac tgagtttaat ttctcggcgt
     9601 gaacggtggt aatttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaagc
     9661 caaataccgg caccatcaac ccgccgataa ccagcccaat accgttgatg gagtccgcca
     9721 ctgccattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa
     9781 ttaccagtag ccagatagcc gcaccgtgag aaatctgtag cgattccccg acgtgaaaca
     9841 ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag acgccggtgg
     9901 caatgaggaa gcagaagtcg atgatcatac gcgtcgtttt atcataacgt tcctccagaa
     9961 aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga
    10021 agatcagcgt caccgctgaa gtcacttccc agcccatcac cgacatgccg cttttgtagg
    10081 cctgcccgga aagaccaacc agttgttccg tggaaaggtt ggttaacatt aacgaagcgg
    10141 caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat
    10201 ctgttttgcg aaccttccac caggtgatga tcgccaccag cagcgtaaaa ccgacaaaac
    10261 tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg
    10321 aatataggga gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc
    10381 gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg
    10441 atattaaaat gaatggaaaa ttgcaaactt cagatgtaaa aaacgaaact ccatacaata
    10501 ttcctttatt gattaacgaa aatgtgatct ccagcggaat ttctctgatc tcgctctggc
    10561 atacctacgc cgacgagcat taccgggtga tctggccacg ggacaagaag aaaccgctta
    10621 tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaagatt cttttaaaga
    10681 atggcgaaca aataacactg catggcaact gtattatatt tttaaagcca atggatattc
    10741 actcttatca ctgtgaaggt ttagtctggg aacaatactg gatggaattt acccccacca
    10801 gtatgatgga tattcccatc ggtcagcaaa gcgttattta caatggcgaa atttataatc
    10861 aggaactcac cgaagttgct gagttaataa cttcaccaga agcaataaaa aataatctgg
    10921 cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgtac gcagacggta
    10981 aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctgc
    11041 aacaacgctg gagcgtagcc gatatggctg ccacgatccc ctgtagcgaa gcctggttgc
    11101 gtcgtctgtt tttacgctat accggaaaga cgccgaaaga atattacctc gatgcgcgtc
    11161 tggatctggc gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa gtcgctgata
    11221 cgctcaactt cttcgactcc tttcatttca gcaaagcctt taaacataaa tttggttatg
    11281 cgccttcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt ccacacaatt
    11341 aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga atagctttgg
    11401 cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc ctgccacagg tgctgatatg
    11461 aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga ctcattaacc
    11521 ctttctgaaa ctgcgggata tcgccgacca ccagtggttc tggtccgttg tgcccgacca
    11581 gacacgggat ctcgaccata gcttcatcat caaagttatg gattgccccg ttgttaggca
    11641 caatcagcaa catccgctcc tgagtgttga aggcaattgc cgccgccaga tcgacgatgt
    11701 atgacgcatg ttcgtcaatt tccagcttgc ccgccgctga atttcctgcc gccgtaatgg
    11761 cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat tacctcattc gcgcgagtat
    11821 gttcagggtt ggaatgctga acgacataat ccgggaagag ataatatttc agataggtgt
    11881 tcggcaatgt atccgggtcc agtgcctgga catcccgcgc ttttgcatag gtgtcgttcc
    11941 agctcgcttc agtatgtgta tcttgctgcg gtggaacata accgtgctta gagacatatt
    12001 gccgcagctg gtgcatcagg tcgttgccct gcaaatcttc aattgatgtc caccagccaa
    12061 agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc ccaacaattt
    12121 gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt ttcgcattcg
    12181 ggcgcagacg acgcgtggct tctgcaacaa ttgctgccgg gttggagtag ttgagcatcc
    12241 aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg ccaatggaac
    12301 gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaacg ccgtggcgca
    12361 gcgggatttt ttcatccagt tcgcgcatcg ggtatttgcc gacgcggatg tgcgccatga
    12421 caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac gcaatatccg
    12481 gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc cgcgaaccat
    12541 cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc aggagcatca
    12601 acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag aatttggtca
    12661 ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa ttaagctatc
    12721 gagctgttca cgcagctggg atacatgcag accaatgatc acctgaatgg catcgccact
    12781 acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg tttgtgacat
    12841 gtcatgcagt gcaatgcgta gacgcgtcgc acaattgtta atgctggaga tattgccgac
    12901 cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg gctctgccgc
    12961 ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca cttccgcatc
    13021 ttcacgtccc ggcgtgcaca tgttgaattg cagaatcagg gtgcggaaaa ccacgaagta
    13081 cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg cgtggttgct
    13141 gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc ccatgttgcc
    13201 caccacacca aagagataca tcacggtcga cattgaggcc gcaagcacag cgtgtaccgc
    13261 aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa ttcccaccag
    13321 catggcggtt aaggttgcgg gaatcagcaa acccgccact tttacccgat tttccggtgc
    13381 ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg agttaccgtg
    13441 cagggcaaaa cctccttccg ggaacaacga tttcagcggc tcggcgctca aactgaactc
    13501 ttgcagatgc tgcgcccagt acatctgaat tccgccttca acagctgccg gaccaaagat
    13561 aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt cgaggaaggt
    13621 gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag attcaatccc
    13681 catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca tcaccaggaa
    13741 ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg gcagtttttt
    13801 atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg cgccgataat
    13861 gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg ccaccgcgtc
    13921 ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat tgatgaaata
    13981 gttccaggtc aggaaactca ccatcaccgc cagacaagca cgcccctgcg cttgcttagc
    14041 aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata ccgtccaacc
    14101 gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg tcagtgattc
    14161 cccgacaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc cggcgaaggg
    14221 aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac tgagcatttc
    14281 cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg gcaggcctgg
    14341 agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt cgtgatcgct
    14401 ttcatgcttt tcgttgggcg gtagagatct actttttcgc gaaaaagtag atctctgtga
    14461 gcactggtac acagcatcga acacatcttt aaaaaaaaga tgttttttca attgattaag
    14521 cagaacttgt gggcgcatta cccgggcttg caggcaaaaa agagatctag agatgatcta
    14581 caaaagcatt gcggagcggt taagaattcg acttaattcc gcggatttca cgctaaacag
    14641 ccttcttccc ggtgaaaaaa aactggcgga agagtttgcg gtatcgcgga tgaccatccg
    14701 taaagcgatt gacctgctgg tagcgtgggg gctggtggtc cgccgccacg gcagcggcac
    14761 ttacctggtg cgcaaagatg tgctgcatca aaccgccagc ctgaccgggc tggtggaggt
    14821 gttaaaacgg cagggaaaaa cagtcaccag ccaggtgctg atttttgaaa tcatgcctgc
    14881 gcctccggca attgccagcc agttgcggat tcaaatcaac gagcagatct acttctcccg
    14941 tcgcgttcgt tttgtggaag ggaaaccgct gatgctggaa gacagctata tgccggtaaa
    15001 actgttccgt aacctttcgc tgcaacatct ggaagggtcg aagtttgaat atattgaaca
    15061 agagtgcggg attttgattg gcggtaatta tgaaagcctg acgccggtgc tcgccgatag
    15121 actgctggcg cggcaaatga aggtagcgga acacacgcca ctgctgcgga tcacctcgtt
    15181 gtcatatagc gagagcgggg agtttttgaa ttattcagtg atgttcagaa atgccagcga
    15241 ataccaggtg gagtaccatt tacggcgact ccacccggac aagagttaac cgatactcca
    15301 gaagagcacc gccagtaatt ggggggtgat aatgcgcagg aacatcacta acggatagac
    15361 agtggcgtaa gagagcgccg ccgcaccgct ggttggatga agattattag caaacgccag
    15421 cgccggagga tcggtcatgg aacctgccag catcccgcac atggtcaggt aattcatttt
    15481 ggctaacatc cgcgccagaa tgccaacagt aatcagcgga acggcggtga tcagggcacc
    15541 ataaccaatc cagcttagcc cttcgccatt gaccagggta ttcacaaaat ccccaccaga
    15601 tttcagacca acgaccgaga ggaacagcac gatccccagt tcccgcagcg cgaggttggc
    15661 acttggcggc ataaaccagt acagcttgcc gatactgccg atacgcccga ggatcaacgc
    15721 cataatcagc ggaccgcccg ccagccccag tttcaacgcg gccgggaatc ctggcacaaa
    15781 gacgggaata gaacctaaca atacgccaag cccgatgcca ataaacactg gcagcatctg
    15841 aacctgttgc agtttttgct gcgcattccc cagcacattg gcaacggcat caattgcgga
    15901 cggacgcccc accaggttga ggatatcgcc gaactgcagg ctgatatcgc cactggcgac
    15961 cagttcgacc ccggcacggt tcaggcgcga gataacaacg tcatagcgtt ctttaaaatg
    16021 caggtcgcga atacgcttac caagcacgtt ttcattggtg accaccacac gctcgacgcg
    16081 caaatcagtg cctttcgtgg acagcgaagt atcgacctcc tgaccaatca ccagttgcgc
    16141 attatgtaaa tccgctggct gacccaccag atgcagcaaa tcgcccagtt ggataatggt
    16201 atctggcgaa ggaactttta gggtttcttc gcgtttcaga cgcgagcaga taattttgtc
    16261 gccgttgaga atcggtacat ctttaatggc taaatcatgc aggttagggt tctcaacgcg
    16321 aatattgata gtcttgatca gcgcgccgcc attggtgcgt gaagactcgt gctgctgggc
    16381 ttctgtctcg acattgacgc ggaaaataac ccgcaacatc cacatggtga acaatatccc
    16441 gcaaatgccg aatggatacg ccatcgcgta actcatcccc atctgatcga ccatttccat
    16501 tggtgtaccc aggtcgcgca aaatctgctg ccctgccccc agcgctggcg tattggttac
    16561 cgcaccggag aaaatcccca gcactaccgg cagtggaata tcaaacagtt tatgcaggat
    16621 ggcggtaacc agaccaccga tgatgacgat cagaacagca aacaggttga ggcgtaatcc
    16681 ggagacgcgc aatgaggcaa agaagcccgg ccctacctga atcccgatgg tataaacgaa
    16741 caggatcagg ccaaattcct gaataacatg cagcatatcg ctacttaatg tcatccccgc
    16801 ctgagaaaca aaatggccga cgatgatccc accaaacagc acgccgccaa tacctaatcc
    16861 tatgccgcga aatttgacgt tgccgataaa caaaccgacg actgccacca aagccaaaat
    16921 actgaccgtt aatgctatat cactcatcgt ccatttctct tgagataaca ttttaattgc
    16981 tacggattat ggctgaatcg gtgaagcaga tttgccttat agcgcacatt atcacgggaa
    17041 agaacgtgcc gaaatatctt aaacagtcga aatttaacgc attgttgaag aagaaagaca
    17101 aaaaaagccc caccagaatg gcggggcaaa gagaatagct agttagctat ttaacgcggg
    17161 acgttcgctg atagcgatac gctgcgctgc gatgggttca ggctcattac gaattaaatc
    17221 aatatgcagt aaaccgttta cgaaggttgc gccagagact tccatatttt cagccagcgt
    17281 aaagctcagg ctaaatggct gattcataag cccttgatgc agccattttt tctcttcttt
    17341 tggctgctcc ggcgtgcctt ttacgctcag gcgcgtacct tccagttgaa tctctaaatc
    17401 ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag tggttatcgt cgcttttctc
    17461 aatgttgtac ggcgggaagc tctgactttc accggcgttt tgcagtgcgt tggccagttt
    17521 gtcaaaaccg atccattgac gcatcagtgg ggataaatcg aagttacgca tagtcatttc
    17581 tccttctaag aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc tccctgacgg
    17641 cgagcatgga gatgtcaggc cgcgcaaggc ggccttagga aattagttga tttcgatacg
    17701 gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg atatacagca aaccatttac
    17761 taggttagca ccacgaacat gaatgttctc agctaactgg aatttgcgtt caaagttgcg
    17821 ttcagcgatg ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt
    17881 caccaccagc agattatcct gggcggtaat ttccagttcg ctctcagcaa aaccagccac
    17941 agcgatagca atgcggtaat ggttttcgtc taccagttca acgttatacg gagggtagcc
    18001 gccattactc tggctctggt tgttttctaa gtggttaaac aaacggtcaa atccaatagc
    18061 agaacggtaa agcggggata aatcaaagtt acgcataatc aatagctcct gaaatcagcg
    18121 agaatgtaag accttccaca atggacaggt caggtagcca gaacacccat caggcgcgtc
    18181 ctcatcggct acgatgtaaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga
    18241 cttttttttg cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag
    18301 tgcgatagcc gccacagagc gacgtaatga tgatgttatt ttcagcaagg gatcgctata
    18361 actcatcagg ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt
    18421 tagcgttcat gatatgcagc ggaatggcat tactcggagg atgctccagc gtgatgtccc
    18481 ataccggagg gaaagaagga acgtatcccg gcacccgcgc cagcgccacc atgattggcg
    18541 atgatgaaac caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg
    18601 tgatggacac gcttttatta ccgtgggatg tgttccgcaa agacagctcg gtgcgctcac
    18661 gcgttgaaaa aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa
    18721 tgcctgataa ctgatttagc gatcagtttc ggtgatccac agctggccgc cttccatcca
    18781 cgccagccac tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg
    18841 gttttcggtt aaatactcaa cctcgccgct ttgcgcatgg gcacaagcaa ttcgattacc
    18901 cagcacaaag cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt
    18961 tttgttatgg gttaactggc gcggctcgcc gccctgtggc gagataagcc acagttgcac
    19021 aatgccgtta tcatctcgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac
    19081 ccagtggcgc gggacgttga ctaaccccgg ataagcccga tggtgggtaa aggttaaacg
    19141 tcgctgcacg atgccacgcg gtggcgcggg cagggttgtt tccgttccac ttaacggcgc
    19201 atcacctgct gctttccagc cagcttcatc ttgcggtaac tcaacgataa acagctccgg
    19261 cactttctcg ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac
    19321 ccatccttct tcataagcac gattgatttc atcgctgcca ggctgcggcg tgggcgtggt
    19381 tttactcacc agcacgcacc agtggctacc gctgtattca cgcggatgct gcttttgtac
    19441 gttgaccggg ccaaacggtg cagcaacccc gacgtttcgc aaatccagcg ccgaatcgaa
    19501 ctgatgcatt acatggtcgt tataggtaaa actcaccatt tcaccgttcg ggctaaagac
    19561 atgcacatgg ctgccgccac gcagcgcccc tgatgtgtac ggaacggtaa tatccattgc
    19621 atcgagattg ttcatcttgc cgccttcaac aatcacgccg cgacggtgat ggaaatcgta
    19681 atgccatgtt tcatgaggat tttcaggtcc gtggataaag acatatttct ctgactttgg
    19741 atgaacggtc accacgccga cgtgcgcgcc ctgtgacacg cgatagataa cctcgacctc
    19801 gccggtatgg atattcacac gctcaatggt ctcgccggta aacgacgcgc cagaaggacg
    19861 cacgtcaaat accggccact ggctgtcggg cgtccaggta ttggtattgg tgagcaggtg
    19921 attacgggga gcaaaggtta tctgtttcat ggcgcggtcc tgatacaaaa cggttcccgc
    19981 catcatactt cacaaggact tcactttcag acaatagtct tttgccatcg tgatgaatgt
    20041 gagtgaaaag atggaacatt tcgacgtggc gattattggc ctcggcccgg cagggtcggc
    20101 gttggcacga aagttagccg gcaaaatgca ggtgatcgcg ctggataaaa agcaccagca
    20161 tggtactgaa ggtttcagca agccttgtgg cggtctgctg gcaccggacg cgcagcgatc
    20221 ttttattcgc gatggactga cgcttcctgt cgatgtgatc gccaatccac agattttcag
    20281 cgtcaaaact gtcgacgtcg ccgcatcgct cacgcgtaac taccagcgaa gctatatcaa
    20341 tattaatcgc catgctttcg acttgtggat gaaatcgctg atccccgcca gcgttgaggt
    20401 ttatcacgat agcctgtgcc ggaaaatctg gcgtgaggat gataaatggc atgtcatttt
    20461 tcgtgcagac ggttgggagc agcatattac tgcccgctat ctggtcggtg cagatggtgc
    20521 caactcgatg gtgcggcgat atctctaccc ggaccatcag attcgtaaat atgtcgctat
    20581 ccagcagtgg ttcgcagaga aacatccggt gccgttctac tcatgcatct ttgataatgc
    20641 gataactgac tgttactcat ggagtatcag caaagacggt tattttatct ttggcggtgc
    20701 ctatccaatg aaagacggtc agacgcgttt cacgacgctg aaagagaaaa tgagcgcctt
    20761 tcaattccag tttggtaagg cggtaaaaag cgaaaaatgc acggtgctat ttccctcacg
    20821 ctggcaggat tttgtctgcg gtaaggacaa cgcctttctg attggtgaag cggcgggatt
    20881 tatcagcgcc agctcgctgg aggggataag ctatgcgctg gatagcgcag agattctgcg
    20941 ttcggtgtta ctgaagcagc cagagaagat caacgcagcc tactggcacg ccacccgcaa
    21001 actgcgttta aaactcttcg gcaagatagt aaaaagccga tgcctgaccg caccggcttt
    21061 aagaaagtgg attatgcgca gtggtatggc gcatattcca cagttgaaag attagccaac
    21121 gcgcgtcaca tcgcccacca gcaggatgta agagagcgcg ccaatcaacg cgacggcgga
    21181 gatataaacc agtgcaggtg cgaacccata gccttgcaac agatattagt ccacaaaaaa
    21241 acacctcata agaaaaggtc ttatatcggg cagaaatctg aaaagggaga tcaggcgcta
    21301 aacaggaggg aaagaagagt atgctttcaa cggcttagct actcgtttaa aggattaatc
    21361 atgaagttga attttaaggg attttttaag gctgccagtt tattcccact ggcgctgatg
    21421 ctttcaggct gtatctcgta tgctctggtt tcccataccg caaagggtag ttcaggaaag
    21481 tatcaatcgc agtcagacac catcactggg ctatcgcagg caaaagatag taatggaaca
    21541 aaaggctatg tttttgtagg ggaatcgctg gattacctta tcactgatgg tgccgatgac
    21601 atcgttaaga tgctcaatga tccagcactt aaccggcaca atattcaggt tgccaatgac
    21661 gcaagatttg ttttaaatgc ggggaaaaag aaatttaccg gcacaatatc gctttactac
    21721 tactggaata acgaagaaga aaaggcactg gcaacgcatt atggttttgc ctgtggtgtt
    21781 caacactgta ccaggtcact ggaaaaccta aaaggcacaa tccatgagaa aaataaaaac
    21841 atggattact caaaggtgat ggcgttctac catccattta aagtgcgatt ttatgaatac
    21901 tattcaccca gaggcattcc ggatggtgtt tccgcagcat tactgccagt gactgttacg
    21961 ctggacatca ttactgcgcc gctgcaattt ctggttgtat atgcagtaaa ccaataatca
    22021 gtaagcgggc aaacgcgttt atgctgtttg cccgcccaca gattaattca gcacatactt
    22081 ctcaatagca aacgccacgc catcttcaag gttggatttg gtgacaaagt tcgccacttc
    22141 tttcaccgaa ggaatagcat tatccattgc cacaccgacg cctgcatatt caatcattgc
    22201 gatgtcgttt tcctgatcgc caatcgccat gatttcttcc ggtttaatac ctaacacgtc
    22261 ggccagtgat ttcacacccg tacctttgtt aacgcgttta tcaaggattt cgaggaagta
    22321 cggcgcactt ttcagcacgg tatatttctc tttcacttcc tgcggaatac gcgcgatagc
    22381 ctggtcgagg atggcgggtt catcaatcat catcactttc aggaactggg tattggggtc
    22441 cattttctcc gcttcgcaga acaccagcgg aatggtggca acgaaggatt catgcaccgt
    22501 gtagtagctg atatcacggt tggcggtgta cagcgtggtg cggtccaggg cgtggaaatg
    22561 agaaccgact tcgcgagaga gtttttccag gaaacgatag tcgtcatagc tgagagcagt
    22621 ttgcgccacg gtgttaccat cagcggcctt ctgtaccagc gcgccgttat aagtaatgca
    22681 gtagtcgccc ggctgttcca tatgcagctc tttcaggtaa ttgtgaacac ctgcataagg
    22741 gcgacccgtc gttagcacga cattcacgcc acgggcgcga gctgcggcaa tcgcattttt
    22801 aacggcaggt gaaatggtgt gatcgggcag cagaagggtg ccatccatat cgatagcaat
    22861 gagtttgata gccatgagtt ccccaggtag attggttcct gacccatgct aacgcgattc
    22921 cgctcaaaaa tcagtacaac acccgaggga aaagggggat gcaacgcggg ttgcatgctc
    22981 cccatttgct tagcggaaga gtttcccttt cagcagttcc atgcctgcgg aaagcagatc
    23041 gttattggct tgtggtgaca cttcaccttg cggtgagagc gcatcaataa tcttcggcaa
    23101 ttgttctgcc agtaaacagg aagccgtact ggtatccacg ccaagttttt gtccaagatc
    23161 ggataccgcg tttgtgccga gtgctgattc cagttgctcg ccgctaactg gttgattgcc
    23221 ctgttgatta ctcagccagg ttgagagaat ggcccctaag ccgccacttt gcagtttttc
    23281 cagcagcacc tgaatgccac cctgctcctc aacccaactt aaaatcgcct gatatttccc
    23341 cgcatcgcct ttcagaaagg cgccgacaac ttcatcaaaa agccccatga taatcacctg
    23401 taaagcgtta cgtgttgacc caaaaagtat agaactgcgg gatgataatt gcggattgca
    23461 gaaataaaaa gggcggagat gatctccgcc cttttcttat agcttcttgc cggatgcggc
    23521 gtgaacgcct tatccggcct acaaaaacat gcaaattcaa tatattgcag gagtctcgta
    23581 ggcctgataa gcgtagcgca tcaggcacgc tcgcattgtt aacgccatta aatatcaata
    23641 ttcgccgctt tcagggcgtt ctcttcaata aacgcacggc gcggttcaac ggcgtcgccc
    23701 atcagcgtgg tgaacaactg gtcagcggca atcgcatcct taacggtaac gcgcagcata
    23761 cgacggcttt ccgggtccat ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga
    23821 cctttataac gctggatgga gaggccgcga cgggactctt tcaccagcca gtccagcgcc
    23881 tgctcgaagc tggctaccgg ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc
    23941 agcaagccac gcagtttctc acccagcgtg cagatacgac gatattcgcc accagtgata
    24001 aactcgtgat ccagcggata gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc
    24061 tcgaacaggt tttgttcggc attggtgtgg acatcaaact tccactggct gccgtgctgt
    24121 tctttgtcgt tcagttcgct gaccagcgcg ttcacccacc gggtaacggt ctgctcatca
    24181 gagaggtcgg cttccgtcag cgtcggctga tagataagct ctttcagcat tgctttcgga
    24241 taacgacgct ccatgcggtt gatcattttc tgcgtcgcgt tgtactcaga caccagtttc
    24301 tctaacgctt cgccagccaa tgccggtgcg ctggcgttgg tgtgcagcgt tgcgccgtcc
    24361 agcgcgatag agatctggta ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc
    24421 ttgcctttct tcactttgta cagcggcggc tgagcgatgt agacgtgacc gcgttcaaca
    24481 atttccggca tctgacgata gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg
    24541 acgtccgcat cggtcatgat gatgatgctg tgataacgca gtttgtccgg gttgtactcg
    24601 tcacgaccga taccacaacc aagcgcggtg ataagcgtcg ccacttcctg agaagagagc
    24661 atcttatcga agcgcgcttt ctcgacgttg aggattttac ccttcagcgg cagaatcgcc
    24721 tggttcttgc ggttacgccc ctgcttcgca gagccgcccg cggagtcccc ttccacaagg
    24781 tacagttcgg aaagcgccgg atcgcgttcc tggcagtctg ccagtttgcc cggcaggccc
    24841 gctaagtcga gcgcaccttt acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg
    24901 gcacgggcag catcgataat tttgccaacc acgattttcg cgtcagttgg gttttccagc
    24961 aggtattccg ccaacagttc gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa
    25021 accagtttgt ctttggtctg ggaggagaat ttcgggtccg gcacttttac ggaaacgacc
    25081 gcaatcaggc cttcacgcgc atcgtcaccg gtggcgctga ctttggcttt tttgctgtag
    25141 ccttctttgt ccatgtaggc attcagggta cgggtcatcg ccgcacggaa gcctgccagg
    25201 tgagtaccgc cgtcacgctg cggaatgttg ttggtaaagc agtagatgtt ttcctggaag
    25261 ccatcgttcc actgcagcgc cacttcaacg ccgataccgt ctttttcggt ggagaagtag
    25321 aagatatttg ggtggatcgg cgttttgttc ttgttcagat attcaacgaa cgctttgatg
    25381 ccgccttcat agtggaagtg gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa
    25441 acgccggagt tgaggaacga caactcacgc agacgtttcg ccagaatttc atattcgaac
    25501 tcggtcacat tggtgaaggt ttcgaggctg ggccagaaac gcaccatggt gccggttttt
    25561 tcagtctcgc cggtaaccgc cagcggtgcc tgcggtacac cgtgttcgta gatctgacgg
    25621 tgaattttac cctcgcgctg aataaccagc tccagttttt gcgacagggc gtttactacc
    25681 gaaacaccaa cgccgtgcag accgccggac actttatagg agttatcgtc aaatttaccg
    25741 cctgcgtgca gaacggtcat gatcacttcc gccgccgata cgccctcttc cgggtgaata
    25801 ccggtcggaa tgccgcgccc gtcatcctgt acagagacag agttatcggc gtgaatggtg
    25861 acgataattt ctttacagtg acccgcgagc gcttcgtcga tagcgttatc taccacctcg
    25921 aataccatgt ggtgcagacc ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc
    25981 ttacgcaccg catccagccc tttcaggact ttgatactgg aggagtcata agaattcgac
    26041 atcaacgttt ctcgctcatt tatacttggg ttaatccgtt attttaccct tttccacggt
    26101 aaacatcttc gaattttcgt ccgacatgtc tataacgtgt tcagcactga tcgcgctgac
    26161 aaagacctgc gattgcgtag cttttaagcg gctggcaagc agcccgcgac gctcatcatc
    26221 aagctcagag gcaaaatcat ctatcaggta gagacaccgc cgcccgcttt cacgggtgag
    26281 gaactctcct tgcgccagac gtaaggcgca catcaacagc ttaagctgcc cacgcgataa
    26341 ggtatcttcc accggcgcac cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg
    26401 cgcggtgtag gttagctggc gatcgcgttc aaaattacgt tccagcacct cagcatattc
    26461 tgtctctttc tcccagccgc gctggaaaga gaaagtcaga gaaaactcag ggagaaattg
    26521 cttacaggta tcagccatat cggccgcgat accggcgcta tactccgcgc gccaggtact
    26581 gatttgctcc gccagcggga tcagttcttt atcccacggg cgtagctgtt cgtaacgtgt
    26641 cacctggcgc agcgccgcat tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt
    26701 gaaaaatccg ggttcgttgt gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg
    26761 gccgccgttg agtaaagtaa acccttctgg cgttatcagc tgcattggca tcaggtgcgc
    26821 cagttccgcg actttatgcc cgtcagtacc gtcgatgcgg actttgctgt cgccctgttt
    26881 gtctttggtt aagccaatcg ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac
    26941 aaacgcctcc tgctcgtggc gaatgacgcg accaatctgc aaactgcgaa acgcccgacc
    27001 atggccgagc gtatagatgg cttccagcac gctggttttg ccactgccgt tggcacctac
    27061 cagaaagtta aagccgggag ataaagcgag atccgcggtt tcaatgttgc ggaaatcgcg
    27121 gatcaacaag cgggtgaggg acattacagt ctcattggca tgacaacata agccgcgctc
    27181 tggctggctg catcttcaat ctgcacgctg gaaaccgaat cggtcagcat catgcggacg
    27241 ttttcgcatt tcagcgcgtt cagaacatcc agcacatagc tgacgttgaa gccgatttcc
    27301 atctccgcac cgctataggt aacgtcgagg atctcttccg cttcttcctg ttccgggttg
    27361 ttggcggtga ttttcagctg gttttcgctg acatacaggc gcacaccgcg gaatttctcg
    27421 ttagagagaa ttgccgcgcg agcaaacgcc tgcttgagga gatcgcagcc tgcttccaga
    27481 tgtttgtccg gattcttcgg cagaacgcgg cggtaatccg ggaagcgacc atccaccagt
    27541 ttagaggtga agataaagtc gccaacgtgg gcgcgaatat tgttgctgcc aatctgcaca
    27601 cgcagcggat tatcgccgcc gtcgagcata cgcatcagtt caatcacgcc tttacgcggc
    27661 acgatcaccg aatggcttgg caaagattga ccaattggca ttgaacagac cgccagacgg
    27721 tggccgtcgg tcgctacggt gcgcagttct tcaccttcgg tttcaaacag cataccgttt
    27781 aagtaatagc gaacgtcctg atgcgccata gaaaactggg tcgcttcaat cagacgcttc
    27841 atcgtcgcct gcggcagggt aaattcgact tcactctgcc agtcatcgag gttcgggaaa
    27901 tccgccgctg gcagggtaga cagcgaaaaa cggctacgcc cggagcgcac cagcatccgc
    27961 tcaccttcca gctgcacggc aatttccgcg ccttcaggca gaccacggca gatatcaaag
    28021 aatttgcgcg ccggaacggt cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt
    28081 gccaccattt ccatctcgag atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt
    28141 aacagcagat taccgagaat cggtagcgta ggacgaccac ctaacggacc gctcacctgt
    28201 tgtagcggtt ttaataaatg ctcacgttct acggtaaatt tcataggttt acgatgacaa
    28261 tgttctgatt aaatttgaaa aatcttcttt gatatcgtgg ctctcttcac gcagctgctc
    28321 gatcttccgg caggcatgaa gcaccgtagt gtggtcacga ccaccaaacg catcgccaat
    28381 ctctggcaga ctgtggttag tcagctcttt cgccagcgcc atcgccatct gacgcggacg
    28441 agccaccgag cgggatcgac gcttggaaag gagatccgcg actttgatct tgtagtactc
    28501 cgccaccgtc ttctgaatat tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc
    28561 gcgcagcgcc tcacgcacga agtcgatagt gatcgcccgt ccggtaaagt tggcgttggc
    28621 aatgacgcgg ttcagcgcgc cttccagctc acgtacgtta gatcgtagac gcttggcgat
    28681 aaagaacgcc acttcgcctg gcaaacgaat gtcattttcg tcggcctttt tcatcaggat
    28741 cgccacgcgg gtttctaact ctggcggttc gatcgccaca gtcagccccc agccgaagcg
    28801 ggatttcaaa cgatcctcaa cgccgttgat ctctttcgga tagcgatccg aggtgagaat
    28861 gatctgttga ttaccttcca gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg
    28921 ttctttatta gcaaaaaact gaatatcgtc gatcagcagt gcatctacgg aacggtagta
    28981 gcgtttaaac tcttcgatcg cgttgttttg cagggcttta accatgtcct gaacaaagcg
    29041 ctcggagtgc atataaacca ctttggcatt cggcttgcgc gccataatgc cgttacccac
    29101 cgcatgcagc aggtgagttt tacccagacc cgtgccgcca taaaggaaca acgggttata
    29161 ggcaccgcca gggttatctg ccacctggcg agccgccgcg cgcgccagtt ggttagattt
    29221 accttcaacg aagttatcaa acgtgtgttt gacgtttacg ttagaacgat aggtcggttc
    29281 tgccggggcc gggacgttat cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg
    29341 cgtttgcgcc acctgtgcag gggccgcgac gttgctcgtc actgccgctt gtggcgtttg
    29401 cgtcaccgat ttggtgccga cttcaaaacg cagctgtggg gcatccgctc cgcagaaact
    29461 ggttagcagt ccattgatat tattaaggta cttgtcccgt acccaatcga ggacaaaacg
    29521 gtttggcgcg tacagggcca gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca
    29581 catactgaat tctgtggctg gtaactcatc ctgcaatcgg gcaagacact gctgccaaag
    29641 cgaaagtgac acggcggact ccactcgaac aaaagtcgat aatgactaag gctgaaacat
    29701 tcatgattgt tgacgtacgt cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc
    29761 tgcggttata tgcccgatca agatcctgca aaacgatcgg gaccgcggat catagcctaa
    29821 actgcgcaag agatcttctg tttctcacag attttcccga tttatccaca ggatgatcca
    29881 gaactcgcta agtgtaaacg atcctgccgc gaggcgggca cgatttacgc cgcatattgg
    29941 aaaatttaat gaccatagac aaaaattggc ttaatcgatc taataaagat ccaggacgat
    30001 ccttgcgctt tacccatcag cccgtataat cctccacccg gcgcgccatg ctggttttca
    30061 ctggtgtgag gccgtacgtt ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga
    30121 aagagaattg actccggagt gtacaattat tacaatccgg cctctttaat cacccatggc
    30181 ttcggtgtcc atcgtttcat ttttcggcgg atatccaata aagccattga atttattcaa
    30241 gtttaggtag aaatcgccat gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt
    30301 tctcacggct tccgtgctcg tatggctact aaaaatggtc gtcaggttct ggcacgtcgt
    30361 cgtgctaaag gccgcgctcg tctgaccgtt tctaagtaat aaagctaacc cctgagtggt
    30421 taagctcgca tttcccaggg agttacgctt gttaactccc agtcaattca cattcgtctt
    30481 ccagcagcca caacgggctg gcacgccgca aattaccatt ctcggccgcc tgaattcgct
    30541 ggggcatccc cgtatcggtc ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg
    30601 caatcggatt aaacgtctga cgcgtgaaag cttccgtctg cgccaacatg aactcccggc
    30661 tatggatttc gtggtggtgg cgaaaaaagg ggttgccaac ctcgataacc gtgctctctc
    30721 ggaagcgttg gaaaaattat ggcgccgcca ctgtcgcctg gctcgcgggt cctgatagcc
    30781 ctcattcggg tctatcaacg cctgattagt ccgctactcg ggccgcattg tcgtttcact
    30841 ccaacctgtt caagctacgg aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt
    30901 tggttgacgg tgaaacgcgt attaaaatgc caccctttac accctggtgg tgacgatccc
    30961 gtcccgcccg gaccatttaa taccagagaa cactaacgat ggattcgcaa cgcaatcttt
    31021 tagtcatcgc tttgctgttc gtgtctttca tgatctggca agcctgggag caggataaaa
    31081 acccgcaacc tcaggcccaa cagaccacgc agacaacgac caccgcagcg ggtagcgccg
    31141 ccgaccaggg cgtaccggcc agtggccagg ggaaactgat ctcggttaag accgacgtgc
    31201 ttgatctgac catcaacacc cgtggtggtg atgttgagca agctctgctg cctgcttacc
    31261 cgaaagagct gaactctacc cagccgttcc agctgctgga aacttcaccg cagtttattt
    31321 atcaggcaca gagcggtctg accggtcgtg atggcccaga taacccggct aacggcccgc
    31381 gtccgctgta taacgttgaa aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc
    31441 aggtgccgat gacgtatacc gacgcggcag gcaacacgtt taccaaaacg tttgtcctga
    31501 aacggggtga ttacgctgtc aacgtcaact acaacgtgca gaacgctggc gagaaaccgc
    31561 tggaaatctc cacctttggt cagttgaagc aatccatcac tctgccaccg catctcgata
    31621 ccggaagcag caacttcgca ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg
    31681 agaagtacga gaaatacaag ttcgatacca ttgccgataa cgaaaacctg aacatctctt
    31741 cgaaaggtgg ttgggtggca atgctgcaac agtatttcgc gacggcgtgg atcccgcata
    31801 acgacggtac caacaacttc tatactgcta atctgggtaa cggcatcgcc gctatcggct
    31861 ataaatctca gccggtactg gttcagcctg gtcagactgg cgcgatgaac agcaccctgt
    31921 gggttggccc ggaaatccag gacaaaatgg cagctgttgc tccgcacctg gatctgaccg
    31981 ttgattacgg ttggttgtgg ttcatctctc agccgctgtt caaactgctg aaatggatcc
    32041 atagctttgt gggtaactgg ggcttctcca ttatcatcat cacctttatc gttcgtggca
    32101 tcatgtaccc gctgaccaaa gcgcagtaca cctccatggc gaagatgcgt atgctgcagc
    32161 cgaagattca ggcaatgcgt gagcgtctgg gcgatgacaa acagcgtatc agccaggaaa
    32221 tgatggcgct gtacaaagct gagaaggtta acccgctggg cggctgcttc ccgctgctga
    32281 tccagatgcc aatcttcctg gcgttgtact acatgctgat gggttccgtt gaactgcgtc
    32341 aggcaccgtt tgcactgtgg atccacgacc tgtctgcaca ggacccgtac tacatcctgc
    32401 cgatcctgat gggcgtaacg atgttcttca ttcagaagat gtcgccgacc actgtgaccg
    32461 acccgatgca gcagaagatc atgaccttta tgccggtcat cttcaccgtg ttcttcctgt
    32521 ggttcccgtc aggtctggtg ctgtactata tcgtcagcaa cctggtaacc attattcagc
    32581 agcagctgat ttaccgtggt ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat
    32641 cctgattcgg tgagttttcg ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc
    32701 gtagggcgga taagcaccgc gtatccgcca cacaaagcaa caggaacatc atgagcgata
    32761 atgacactat cgtagcccag gccacgcctc cgggacgtgg cggcgttggc atcctgcgca
    32821 tctccggctt caaagcccgt gaagttgccg aaaccgtgct gggtaaactg cctaagccgc
    32881 gctacgccga ttatcttccg tttaaagacg ccgatggcag cgtgctcgat caggggattg
    32941 cgctatggtt ccctggcccg aactcgttca ccggcgaaga tgtgctggaa ctgcagggtc
    33001 atggcggtcc ggtgattctc gacctgctgt taaaacgcat tctgaccatt cccggcctgc
    33061 ggattgctcg ccctggtgag ttttccgaac gcgcgtttct taacgataaa ctcgacttag
    33121 cccaggccga ggcgattgcc gatcttatcg atgccagttc ggaacaggca gcccgttcgg
    33181 cacttaactc gctgcaaggc gcattctccg cacgggtcaa ccatctggtg gaagccctca
    33241 cccacttgcg catttacgtc gaagcggcaa ttgatttccc cgatgaagag atcgatttcc
    33301 tctccgacgg aaaaattgaa gcccagctta atgacgtgat tgccgatctc gatgcagtgc
    33361 gtgctgaagc acgtcagggt agtttgttgc gcgaagggat gaaggtggtg attgccggac
    33421 gtcctaatgc cggtaaatcg agcctgttaa acgcgctggc tggccgtgaa gcagcaatcg
    33481 taaccgatat cgccggaacc acgcgtgacg tgttgcgtga gcatatccac attgacggaa
    33541 tgccgctgca tatcatcgac accgccgggc tacgtgaagc cagcgatgaa gtggaacgta
    33601 ttggtatcga gcgcgcgtgg caagaaattg aacaagccga ccgcgtgctg tttatggtcg
    33661 atggcaccac gacagacgcc gttgatccgg cggagatctg gccggaattt atcgcccgtc
    33721 tgcccgcgaa actgccgatc accgtggtac gcaataaagc cgatatcact ggcgaaacgc
    33781 tgggaatgag tgaagtgaac ggtcatgcgt tgattcgtct ctcggcaaag actggcgaag
    33841 gcgtggacgt gctgcgtaac catctcaaac agagcatggg ctttgacacc aacatggaag
    33901 gcggcttcct ggcgcgtcgt cgccaccttc aggcgctgga acaggcagcg gagcatttgc
    33961 agcaaggtaa agcgcaactg ttgggtgcct gggcgggcga actgctggcg gaagagttgc
    34021 gcctggcaca gcagaactta agcgaaatca ccggggaatt tacctctgat gatttgctgg
    34081 ggcggatttt ctccagcttc tgtattggta agtaagtcgt ctaaattgat ataaagggca
    34141 ctgttccgtt ctgggggaaa ctaactatca gaacggaaca gcgcttgttt ttttatgagc
    34201 atcctgcacc aaaactgcct ttactactgg ctggagcgac tttcaacatc ttgcagtcct
    34261 gtctttacag agaacgatat tgctttagcc gattaagctc ctcttgcgga aagttaaatt
    34321 cgtctattgc tttccagaat agttctttaa atacaggatt gccatttgcc ttgcttatta
    34381 catatttgcc ttcattattc ttcgcttcca gttgcatcat cacatcttta agataatttt
    34441 cattttcacc aaccacaatt ttgaatattt tcagccagag acccatatat atcatccgct
    34501 ttctctaccg caagagagaa tgacttccca tcaaagtcac gggtaaactg ctccaaaatc
    34561 tcatcgaaag tgaacccgtg ttgcaacgcc aggcgaatcg ccgcattcag atatacatca
    34621 atgacctgct tactcattgt cttatagctg gcaacataca aagagcaaac tgcattattt
    34681 ctgctcagcg acaggagttc tctcactaca tctttatcat tttcataatg gctggctatc
    34741 acttcagctg tctccatcca ggcagcaaca atcttcgtat gccctttcac tatcgcagaa
    34801 tataagcctg atgccttcgt ccatttgtct gatgctcccg ccactttttc tcttgctgcc
    34861 atcagtttca atatatcttc tttatttaat tcatttttaa acattttggc ttttatattg
    34921 gctagcaata tattaacaac attataatca ttggttctaa tagcgctatt taaatcatca
    34981 attgagtcta tttcctttcc tatacgaaga agtatttcat ttttatttac cccaaattct
    35041 ttctcaaagt catctatcgc caacaaccat gctttgacaa ttttattttg attatttttc
    35101 aaagccctgg gaatccagct cactccgaga ttatctttcg caaggagtag agtttccagg
    35161 tcctcttggc taaaattata ttttctggtc aactctttta aattt
//
LOCUS       DAZFKY010000027        34868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFKY010000027 DAZFKY010000000
VERSION     DAZFKY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHKCL_001209"
     CDS             complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHKCL_001209"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5762217.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1008..3449)
                     /gene="rnr"
                     /locus_tag="ACHKCL_001210"
     CDS             complement(1008..3449)
                     /gene="rnr"
                     /locus_tag="ACHKCL_001210"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5762218.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3488..3913)
                     /gene="nsrR"
                     /locus_tag="ACHKCL_001211"
     CDS             complement(3488..3913)
                     /gene="nsrR"
                     /locus_tag="ACHKCL_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5762219.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4118..5416)
                     /gene="purA"
                     /locus_tag="ACHKCL_001212"
     CDS             complement(4118..5416)
                     /gene="purA"
                     /locus_tag="ACHKCL_001212"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5762220.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5520..5717)
                     /gene="yjeT"
                     /locus_tag="ACHKCL_001213"
     CDS             complement(5520..5717)
                     /gene="yjeT"
                     /locus_tag="ACHKCL_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5762221.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5799..6803)
                     /gene="hflC"
                     /locus_tag="ACHKCL_001214"
     CDS             complement(5799..6803)
                     /gene="hflC"
                     /locus_tag="ACHKCL_001214"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5762222.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6806..8065)
                     /gene="hflK"
                     /locus_tag="ACHKCL_001215"
     CDS             complement(6806..8065)
                     /gene="hflK"
                     /locus_tag="ACHKCL_001215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5762223.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8151..9431)
                     /gene="hflX"
                     /locus_tag="ACHKCL_001216"
     CDS             complement(8151..9431)
                     /gene="hflX"
                     /locus_tag="ACHKCL_001216"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5762224.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9508..9816)
                     /gene="hfq"
                     /locus_tag="ACHKCL_001217"
     CDS             complement(9508..9816)
                     /gene="hfq"
                     /locus_tag="ACHKCL_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5762225.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9902..10852)
                     /gene="miaA"
                     /locus_tag="ACHKCL_001218"
     CDS             complement(9902..10852)
                     /gene="miaA"
                     /locus_tag="ACHKCL_001218"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5762226.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10845..12692)
                     /gene="mutL"
                     /locus_tag="ACHKCL_001219"
     CDS             complement(10845..12692)
                     /gene="mutL"
                     /locus_tag="ACHKCL_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5762227.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SENAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12702..14039)
                     /gene="amiB"
                     /locus_tag="ACHKCL_001220"
     CDS             complement(12702..14039)
                     /gene="amiB"
                     /locus_tag="ACHKCL_001220"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5762228.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14058..14519)
                     /gene="tsaE"
                     /locus_tag="ACHKCL_001221"
     CDS             complement(14058..14519)
                     /gene="tsaE"
                     /locus_tag="ACHKCL_001221"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5762229.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14491..16038)
                     /gene="nnr"
                     /locus_tag="ACHKCL_001222"
     CDS             complement(14491..16038)
                     /gene="nnr"
                     /locus_tag="ACHKCL_001222"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5762230.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16037..17176
                     /gene="queG"
                     /locus_tag="ACHKCL_001223"
     CDS             16037..17176
                     /gene="queG"
                     /locus_tag="ACHKCL_001223"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5762231.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17159..17212)
                     /locus_tag="ACHKCL_001224"
     CDS             complement(17159..17212)
                     /locus_tag="ACHKCL_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762232.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17446..17521)
                     /locus_tag="ACHKCL_001225"
     tRNA            complement(17446..17521)
                     /locus_tag="ACHKCL_001225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17486..17488),aa:Gly,seq:gcc)
     gene            complement(17557..17632)
                     /locus_tag="ACHKCL_001226"
     tRNA            complement(17557..17632)
                     /locus_tag="ACHKCL_001226"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17597..17599),aa:Gly,seq:gcc)
     gene            complement(17843..18388)
                     /gene="orn"
                     /locus_tag="ACHKCL_001227"
     CDS             complement(17843..18388)
                     /gene="orn"
                     /locus_tag="ACHKCL_001227"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5762233.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18483..19535
                     /gene="rsgA"
                     /locus_tag="ACHKCL_001228"
     CDS             18483..19535
                     /gene="rsgA"
                     /locus_tag="ACHKCL_001228"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5762234.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19632..20600
                     /gene="asd"
                     /locus_tag="ACHKCL_001229"
     CDS             19632..20600
                     /gene="asd"
                     /locus_tag="ACHKCL_001229"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5762235.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20622..23945
                     /gene="mscM"
                     /locus_tag="ACHKCL_001230"
     CDS             20622..23945
                     /gene="mscM"
                     /locus_tag="ACHKCL_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5762236.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24096..25598)
                     /gene="yjeM"
                     /locus_tag="ACHKCL_001231"
     CDS             complement(24096..25598)
                     /gene="yjeM"
                     /locus_tag="ACHKCL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5762237.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25817..26794)
                     /gene="epmA"
                     /locus_tag="ACHKCL_001232"
     CDS             complement(25817..26794)
                     /gene="epmA"
                     /locus_tag="ACHKCL_001232"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5762238.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27119..28927
                     /gene="frdA"
                     /locus_tag="ACHKCL_001233"
     CDS             27119..28927
                     /gene="frdA"
                     /locus_tag="ACHKCL_001233"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5762239.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            28920..29654
                     /gene="frdB"
                     /locus_tag="ACHKCL_001234"
     CDS             28920..29654
                     /gene="frdB"
                     /locus_tag="ACHKCL_001234"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5762240.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPTQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29665..30060
                     /gene="frdC"
                     /locus_tag="ACHKCL_001235"
     CDS             29665..30060
                     /gene="frdC"
                     /locus_tag="ACHKCL_001235"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5762241.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30071..30430
                     /gene="frdD"
                     /locus_tag="ACHKCL_001236"
     CDS             30071..30430
                     /gene="frdD"
                     /locus_tag="ACHKCL_001236"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5762242.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30493..31626
                     /gene="ampC"
                     /locus_tag="ACHKCL_001237"
     CDS             30493..31626
                     /gene="ampC"
                     /locus_tag="ACHKCL_001237"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5762243.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31714..32247
                     /gene="blc"
                     /locus_tag="ACHKCL_001238"
     CDS             31714..32247
                     /gene="blc"
                     /locus_tag="ACHKCL_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5762244.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32244..32561)
                     /gene="sugE"
                     /locus_tag="ACHKCL_001239"
     CDS             complement(32244..32561)
                     /gene="sugE"
                     /locus_tag="ACHKCL_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5762245.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32737..32883)
                     /gene="ecnB"
                     /locus_tag="ACHKCL_001240"
     CDS             complement(32737..32883)
                     /gene="ecnB"
                     /locus_tag="ACHKCL_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5762246.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(32994..33119)
                     /gene="ecnA"
                     /locus_tag="ACHKCL_001241"
     CDS             complement(32994..33119)
                     /gene="ecnA"
                     /locus_tag="ACHKCL_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5762247.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33171..33737)
                     /gene="efp"
                     /locus_tag="ACHKCL_001242"
     CDS             complement(33171..33737)
                     /gene="efp"
                     /locus_tag="ACHKCL_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5762248.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33779..34807
                     /gene="epmB"
                     /locus_tag="ACHKCL_001243"
     CDS             33779..34807
                     /gene="epmB"
                     /locus_tag="ACHKCL_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5762249.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8280 a         9566 c         8885 g         8137 t
ORIGIN      
        1 taataaaatg aatatttgca atacgtaatt atcttaccag ctatagacaa aaaaaaacca
       61 tccaaatctg gatggctttt cataattcag aggaactagc tgcgctgacg aaccgcttca
      121 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
      181 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
      241 cccatcacca gcgccaggcg tccggtcatt ttgctttgat agagcgtatg atccgcctca
      301 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
      361 ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgtg
      421 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc
      481 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
      541 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctggta ctgacgtcct
      601 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
      661 tggcggtttg ccaactggat aaccacgccc tgggactcaa gggcgtggat cagcggtaac
      721 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
      781 tccagcaggg cctgcactgc atggatgccg taaatcattt cgctcattaa tgtactcgtt
      841 gtttacgttt tgcctgatgc actacgttta tccggtctac atgatccctg caatatattg
      901 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
      961 gcattttgtc agcaatctaa ccctcttctg tttgaagagg gtaatgatca ctctgccact
     1021 tttttcttcg ccgcacgctt cgctttggtc gctgcagcta ttttctgcgt tttcgccgat
     1081 ggctttttcg cttttctcgc gtctttcttc gctgctttcg gcttcgtttt tttctcaccg
     1141 cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct
     1201 tttttacctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt
     1261 gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg
     1321 acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc acccatcagg
     1381 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccagc
     1441 ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt
     1501 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagac
     1561 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccaattgc
     1621 agcatctctt ccatcgaata atggtagcca ccggtttcag tggtgttgcc ctgatgcccc
     1681 tgctctttcg ccagcagata cttaatggcg cggtgcagca tcaggtcagg ataacgacga
     1741 atcggcgaag taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttcc
     1801 ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca
     1861 ggacggtcgg caaccgactc cagcagctcc gcgtagtcgc gcggttccgg cttgttaccg
     1921 cccggcagtt ccagccccag ctctgccagc actgaacgga aagaggtaat cgcttcggtg
     1981 ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc
     2041 gccgagatat tcgccagaat catgcactct tcaattaact tgtgtgcgtc gttacgctga
     2101 gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc
     2161 tcaaatgaga tcccgccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc
     2221 aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc
     2281 agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
     2341 aatttgtagc cagtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
     2401 cggtctacct gcggattgag cgagcacaga ccgttagaga gcacttccgg cagcatcggg
     2461 ataacctgcg aagggaagta caccgacgta ccacggttac gcgcttctct gtccagcggc
     2521 gttggcggac gcacatagta gctgacgtca gcaatcgcga cccataaacg ccagccgccg
     2581 ccgcgttttt tctcgcagta aaccgcatcg tcaaagtcac gggcgtcttc gccatcaatg
     2641 gtgaccagcg gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc cggcacttct
     2701 tctttcagcc cagcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
     2761 gtacgcagag cgatatcaac cgccatgccc gtgcccatat tgtcgcccag cacttcgacg
     2821 attttaccca ccgctttggt acggcgagtc gggcgttggg tcagttcaac aaccaccaca
     2881 aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg
     2941 ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg
     3001 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagca
     3061 cccagcggct gagccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
     3121 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
     3181 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagta
     3241 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
     3301 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
     3361 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
     3421 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
     3481 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
     3541 caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt
     3601 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
     3661 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaggcgaa
     3721 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
     3781 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
     3841 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
     3901 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
     3961 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
     4021 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
     4081 agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac gggtcgcgca
     4141 gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggt acaccggtca
     4201 gctcttcaat gcgcttgatg tagttcagcg ccgcctgcgg caggccgcta cgatctttca
     4261 cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
     4321 agtcgtcagc tgccagcgga gtggtagtca cttcacgacc atccggcata cggtaagcca
     4381 cacagagttt cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
     4441 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
     4501 gacgacgacc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
     4561 catcaaacag ttcagtcggg aacggacctg cacccacacg agtggagtaa gctttgagga
     4621 tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
     4681 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
     4741 cctgcgcacc ttcgaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
     4801 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
     4861 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
     4921 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
     4981 cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
     5041 ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacatgataa tcaaggatca
     5101 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
     5161 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctag
     5221 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
     5281 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
     5341 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
     5401 cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct
     5461 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
     5521 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
     5581 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
     5641 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
     5701 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg
     5761 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
     5821 gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
     5881 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
     5941 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccgcg catgatgcgg
     6001 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
     6061 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
     6121 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttaattcgc
     6181 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
     6241 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgccgccggg
     6301 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
     6361 gtcagacgac cacgggaatc ggtgacgatg tctttcacgt ccaggcgacc aatttcagaa
     6421 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgcccccc
     6481 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg
     6541 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
     6601 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
     6661 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
     6721 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
     6781 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
     6841 cattggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
     6901 cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggca ccgttatcgc
     6961 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
     7021 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
     7081 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
     7141 caaaacgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
     7201 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
     7261 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
     7321 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca
     7381 tgtcatacgg acgaatcgtc tcttccagtt cgcgctgagt atcgctacga atcacggtac
     7441 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aactccacgc agggcgctgt
     7501 cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat
     7561 tggtgacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
     7621 tcacaccaga agctgccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga
     7681 taaacgtcgg tttccagttc agacctggct caaccagatg gctgaattta ccaaagcgtg
     7741 ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
     7801 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
     7861 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc
     7921 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
     7981 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
     8041 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
     8101 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
     8161 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
     8221 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
     8281 taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc
     8341 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
     8401 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
     8461 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
     8521 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca
     8581 atcacatgca gcagcaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc
     8641 accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg
     8701 acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
     8761 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
     8821 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
     8881 tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga
     8941 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac caatcccgcc tttctgtctt
     9001 tcaaggtggg tccagccacg caccaggcgt gtagccagat ggcgcagctg cgccagctca
     9061 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
     9121 gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
     9181 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
     9241 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
     9301 acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc
     9361 atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
     9421 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
     9481 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
     9541 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
     9601 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
     9661 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
     9721 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
     9781 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
     9841 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
     9901 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
     9961 cactgtcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
    10021 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgcctccaa
    10081 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    10141 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    10201 aacgctgctc aattcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    10261 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    10321 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    10381 ctgccgcaac cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    10441 ccgcctgttg ctcaattctg gcccgtactt ccgggtctgc cgacggtagc ggcgacaacc
    10501 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    10561 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    10621 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    10681 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    10741 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    10801 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    10861 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    10921 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    10981 tgcgcatttt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    11041 acggactgct tcgctaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    11101 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    11161 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    11221 tttagccgca acggaatcag caacggctgg gcgcaaacgg gcgcttcacc cggcgtcagt
    11281 tgtacctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg
    11341 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    11401 ttcgccgcaa gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat cggcgcgggc
    11461 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtaacctgg ctgcgcattc
    11521 ggccaggggg cagccggacg actacctgat gctggcgcag gagtgtagcg cggagctact
    11581 ggctcacgaa ctgccggttc agcaaagtga ttgcgccccg ccgccacgcg gttttccgga
    11641 atgggacgcg gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt
    11701 tgtagcacgc tcagcacgcc ctgatagata aagtcatgca ccagacgcga ctgatgaaaa
    11761 cgcacttcgt gtttggcggg gtgcacgttg acgtccacct gatgcgggtc gatctccaga
    11821 tacaacacaa atgcaggttg ctgatcggcc cccagtttgt cttcacaggc ctggcggatc
    11881 gcgtgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc
    11941 gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgcagcgt gagatcgccg
    12001 tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccacaaat cgcgcctaag
    12061 cgccgttctt tttgcccgcc ttccggcacc gcgcggtact ggcgtacgat tttgccgtta
    12121 tgcgacaggt tgatcgtgac gtcgaaacgc gccagtgcga tgcggcggat gatttcatcg
    12181 atatggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    12241 aacaggtcca gtacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg
    12301 tccatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    12361 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    12421 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc
    12481 gccagcgcca gctcgtcttt tttgatacca cagccgttat cacgaatgcg gataagtttc
    12541 gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc
    12601 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    12661 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga
    12721 tctggcgtcg tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc
    12781 ggatgcgcaa ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg
    12841 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    12901 agtactgatg ggatatccgg cgagcgcaga acgccaaggc tggcgtgttc tggtcgacgt
    12961 ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    13021 acccgctggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac
    13081 tgactgttcg ccagcacatc acccgccccg cccagcagct ccgactgttt ctcgtgctgc
    13141 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    13201 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    13261 tgcttacgtg ccacatcgct gcgccccatc accgagataa agtaatcccc gtcacgggtt
    13321 aaaacgcctt taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    13381 atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg
    13441 tgtccggcat caatagcgat gataatttta tcgccattgt tagccgtcgc gcgcgctgcc
    13501 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    13561 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg taggcgtttc aacgcgtttc
    13621 acaaccacag gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt aaagacgaca
    13681 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg
    13741 accaccagcc gtagcgtttg tgcatcttta ggcgttccgg agcgaatcgc cttcaccaga
    13801 ttattaccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    13861 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    13921 gttatccgcg cctgttggtt gccgttagaa acctgaatat cagagagcgt cgcggcaccc
    13981 acctgcgcgc acagcagcag caacgtcgct accaaccaat ttctgatgcg atacatcatc
    14041 ccaccacctt tcaaaggtta accggctaaa cgtgccagca acaattcacc cgcagaggaa
    14101 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    14161 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    14221 gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    14281 tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg
    14341 cttttgacat taccctgatg gcccaaagcc tgtaaaaagc cccggctaaa ggtggtttta
    14401 cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc
    14461 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
    14521 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    14581 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac
    14641 acagcctgca caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    14701 accagagagc acatcgccca tgccgccgct tgccatgcct gcatttccgg catcaataat
    14761 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    14821 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    14881 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    14941 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    15001 aaaattctca actttttgca gggctttttt cccccactct tgctggccca gaccgggacc
    15061 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg
    15121 caccatcaat tctggtcgtg cagtcagcag cggcgcaatg ttctcactgc gggtcagtac
    15181 tcggactaaa ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc
    15241 cgtgccgtga tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg
    15301 gcgacgcggt ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
    15361 ctgacctgcc agccagctat ccagccccag tgagtcaaaa tgcagttgtc ctgtaacatc
    15421 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
    15481 gttgatcact gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac
    15541 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    15601 gggtgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagagcta ccgactcggg
    15661 ccagacaata ttcgaagcat ggatctcgcc tcccgcgttt aaccatgcct cgcgtgccag
    15721 cgcggcctct tccggcaacg gtttatcgct ctcctgggcc aacaacgtga cctcaatgcc
    15781 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    15841 caacaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    15901 gccagcgcga agcatcagct catagagtgt gagccccagc gcatctgccg cctcgcgttc
    15961 tccgcggcgg atatcgtcgg cgtgccagac ggtgtgtggt atacttacgg ggtttttctt
    16021 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    16081 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    16141 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    16201 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt
    16261 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    16321 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    16381 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    16441 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    16501 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    16561 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa
    16621 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    16681 tgctcgccgc tgtatctctt atctcaccat cgaactggaa ggggcgatcc cggaagagtt
    16741 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    16801 gaatcgctat tcacaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc
    16861 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    16921 ttcggcgatt cgccgtatag gtcacctgcg ctggctgcgt aatatcgccg tggcattagg
    16981 caatgcgccc tgggatgaaa cgattctagc ggcgctcgag agtcgcaaag gtgagcaccc
    17041 acttcttgat gagcacatag cgtgggcgat ggcgcagcaa atcgagaggc gaaatgcgtg
    17101 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtaattg aaaaagggtt
    17161 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    17221 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg aaatcttaat ttttaaatta
    17281 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    17341 cggcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc
    17401 aataacgcat cgcgtcgctg tggatatttt attgagagaa gaatttggag cgggaaacga
    17461 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
    17521 cttgggtggt ctgtgcctta cagcactttc aaattttgga gcgggaaacg agactcgaac
    17581 tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc gcgtactact
    17641 taattttgct ttcgtaattt tcgcatttct gcgtcgttac gggaggcgca ttatacgaga
    17701 aatctttccc tctgcaaccc ccctaaaaat ttttttttga gtttttatct caagtgctga
    17761 aattatcagc aagacgttca atttagcgtc aaaatcacgc cgccgcaagt ggattccggc
    17821 gcttacgtga ccagcaaaaa ttttacagct tgataaaatg ctcgcggtag taagccagct
    17881 ccgccaccga ttcacggata tcatccatcg cctgatgcgt cccctgtttg gtaaaaccat
    17941 caagaatttc cggcttccag cggcgcgcca gctctttcag ggtgctgaca tcgagataac
    18001 ggtagtggaa gtaggcttcc agctccggca tgtatttaaa caggaagcga cgatcctgac
    18061 cgatgctgtt accgcaaatc ggcgatttac ccgcaggcac ccactgtttt aaaaattcga
    18121 gcgttgccag ttcagcttca cgatcgccca tcgtgctcgc tttcacgcgc tccaccagcc
    18181 cgctggcggt atgggtgcgc acgttccagc catccatcag cgccagctgt tcatcggact
    18241 gatgtactgc aatggtcggc ccttctgcca gaatattcag attggcatcg gtcaccagcg
    18301 tggcaatctc aataatgcga tcgcgctcgg gatccagacc ggtcatctca agatcgatcc
    18361 aaatcaggtt gttttcattg gcactcatgc tattttccac ccattcacag tgactatatt
    18421 gatccacaat tgcgtgtatc atagatgttt tgcccatcag gggcgaccag gagtcagtac
    18481 gattgagtaa aaataaactc tccaaaggcc agcagcgccg cgtgaacgcc aatcaccagc
    18541 gtcgtcttaa aacgtctaag gagaagcccg actacgacga caatctgttt ggcgagcctg
    18601 atgaaggtat cgtcatcagc cgctttggta tgcacgctga tgtggaatcc gccgatggcg
    18661 acgttcaccg ctgcaatatt cgccgtacca tccgttcgct ggtgaccggc gaccgcgtag
    18721 tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt caaagggatc gtggaagcgg
    18781 tgcatgagcg cacctcagtg ttgacgcgtc cggatttcta cgacggcgtg aaacctattg
    18841 ccgccaacat cgaccagatt gtcattgtct ccgccatttt gccggagctg tcgctcaata
    18901 ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat tgagccgatt attgtgctca
    18961 acaagataga cctgctggac gacgaaggca tggcgttcgt caacgaacag atggatatct
    19021 accgcaatat cggttatcgc gtattgatgg tttccagcca tactcaggat gggctaaaac
    19081 cgctggaaga ggcgttgacc gggcgcatca gcatttttgc cgggcagtct ggcgtcggca
    19141 aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga gatcctgacc aacgatgtct
    19201 ctgacaactc ggggcttggt cagcacacca cgaccgccgc acgcctgtat cacttcccgc
    19261 acggcggtga tgtgattgat tcccctggtg tgcgtgagtt cggcctctgg cacctggagc
    19321 cggaacaaat cactcagggc tttgtcgaat tccatgacta tttaggtctg tgtaaatatc
    19381 gcgattgcaa acacgatacc gatccgggct gcgctatccg ggaagcggtt gaggaaggaa
    19441 aaatcgcgga aacccgtttc gaaaactatc accgtattct ggaaagcatg gcgcaggtaa
    19501 aaacgcgtaa aaacttttct gatacggatg actgacaatt aagctaaccg tcgttagaat
    19561 cgtccccttt ttttcaggat ccggcattta ggccggatca ggaacgacaa aacaatggcc
    19621 tggaggctac cttgttaaat tcatttaaac tttcgctaca gtacattctg ccgaaactat
    19681 ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc aggatggctg acaaaactgg
    19741 ttatcgatct gttcgttaaa tactacaagg tcgacatgaa agaggcgcaa aagccggaca
    19801 ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc gctgcgtgac gaagtacgcc
    19861 caatcgatac cgatccgaat gtcctggtca tgcctgccga tggcgttatc agccagttgg
    19921 gtaaaatcga agaagataaa atcctgcaag ccaaaggcca caactacagc ctcgaagccc
    19981 tgctggcagg caactatctg atggcggacc tgttccgcaa cggtacgttt gtgaccactt
    20041 acctctcccc gcgtgactac caccgcgtac acatgccgtg caacggtatt ctgcgtgaga
    20101 tgatctacgt gccgggcgat ctcttctccg ttaaccatct cacggctcag aacgtgccga
    20161 atctgtttgc ccgtaacgaa cgcgtgatct gcctgttcga taccgaattt ggcccaatgg
    20221 cgcagattct ggtcggggcg acgattgtag gcagcattga gacggtctgg gcgggcacca
    20281 ttacgccgcc gcgcgaaggc atcatcaagc gctggacctg gcctgccggg gaaaacgacg
    20341 gttctgtggc gctgctgaaa ggccaggaaa tgggtcgctt taaacttggc tctaccgtta
    20401 tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca actggaaagc ctgtctgtca
    20461 cgaaaattgg ccagccgctg gcagtatcta ccgaaacctt tgttacgcca gacgctgaac
    20521 ccgccccgct tcctgctgaa gagatcgaag cagaacacga cgccagccca ttggttgacg
    20581 ataaaaaaga ccaggtctaa tccatcaaag gaaacgctga cgtgcgcctg attatcactt
    20641 ttctgatggc ctggtgcctc agttgggggg cgtacgccgc gacggccccc gatagcaaac
    20701 aaatcaccca ggaactggag caggcaaaag cggcgaaacc cgcgcagcca gaagtcgtag
    20761 aggcactcca gtctgcctta aatgcgcttg aggaacgaaa aggttccctt gagcgcatca
    20821 agcaatatca agaagtcatt gataattatc cgaaactctc cgctactctg cgcgcacaat
    20881 taaacaacat gcgtgacgag ccgcgcagcg tgtcgccggg gatgtctacc gacgcgctga
    20941 atcaggaaat tctccaggtc agcagtcagt tgctggataa aagccgtcag gcccagcaag
    21001 agcaggagcg cgcccgcgag attgccgatt cgctgaatca actgccgcaa cagcaaaccg
    21061 acgcccgccg tcagttaaat gagatcgagc gccgcctggg gacgcttacc ggcaatactc
    21121 cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc tgcacgtctt aaggcgctcg
    21181 ttgatgaact ggagctggcg cagctgtctg ccaataaccg ccaggaatta gcgcgcttgc
    21241 gctctgagct ggctgaaaaa gagagccagc aactggatgc gtatttgcag gccttgcgta
    21301 atcaattgaa cagccaacgc cagcttgagg cagagcgggc gctggaaagt accgaattac
    21361 tagcagaaaa cagtgccgat ttgccgaaag atatcgtcgc gcaattcaaa attaaccgcg
    21421 aactatcggc ggcgctgaat caacaggcgc agcggatgga tctcgttgcc tcgcaacagc
    21481 gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt gaatacgctg cgtgaacagt
    21541 cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt gcgggcgcag gtggcacggc
    21601 tgccggaaat gccgaaacca caacagcttg ataccgaaat ggcgcagttg cgtgtgcaac
    21661 ggttacgtta tgaggatctg cttaataaac agccgctgct acggcaaatt catcaggccg
    21721 acggtcagcc gctgaccgct gagcaaaacc gtattctgga agcacagcta cgcactcagc
    21781 gtgagttgct gaactcattg ttgcagggtg gcgatacgct actgctggaa ctgaccaagc
    21841 tgaaagtctc caacgggcaa ctggaagatg cgctgaaaga agtgaacgaa gcgacccacc
    21901 gctatctgtt ctggacctct gacgtgcgcc cgatgaccat cgcctggccg ctggaaatcg
    21961 cccaggatct gcgtcgtctc atttcgctgg acaccttcag tcagttgggc aaagccagtg
    22021 tgatgatgct gaccagcaaa gagacgattt tgccgctgtt tggcgcgttg attctggtcg
    22081 gttgcagtat ttactcgcgc cgctatttca cccgttttct tgaacgttcg gcggcgaaag
    22141 tcggcaaagt gactcaggat cacttctggc tgacgttgcg cactcttttc tggtcgattc
    22201 tcgtcgcgtc accgctgccg gtgctgtgga tgacgctggg ttacggcttg cgcgaggcgt
    22261 ggccttatcc gctggcggtc gcgattggtg atggcgtcac agccaccgtg ccgctgctgt
    22321 gggtagtgat gatttgcgct acctttgccc gcccgaacgg cttgtttatc gctcattttg
    22381 gctggccgcg cgaacgtgtt tctcgtggga tgcgctacta cctgatgagc atcgggctta
    22441 ttgtgccact gattatggcg ctgatgatgt tcgataacct cgacgaccgc gaattttccg
    22501 gttcgctggg acggctttgc tttatcctca tttgcggtgc gctggcggtg gtcaccctca
    22561 gcctgaaaaa ggccgggatc ccgctgtatc tcaacaaaga aggcagcggc gacaatatta
    22621 ccaaccatat gttgtggaac atgatgattg gcgcgccact ggttgccatt ctggcgtcgg
    22681 cggtgggtta tctggcaacg gcacaggcgc tgttagcgag gcttgaaacc tcggttgcca
    22741 tctggttcct gctactggtg gtttatcacg ttatccgccg ctggatgctg atccaacgac
    22801 gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga gatgttagcg caacgtgcgc
    22861 gtggcgaaga ggaagcacat catcacagta gcccggaagg ggcaattgaa gtcgatgaaa
    22921 gcgaagtcga tctcgatgcc atcagtgcgc agtccttgcg gctagtgcgc tcaattttga
    22981 tgttgatcgc cctgctttcg gtcattgtgc tgtggtcaga aatccattcc gctttcggct
    23041 tcctcgaaaa tatttcgctg tgggatgtca cctccacggt acagggcgta gaaagtctgg
    23101 agccgattac cctcggtgcg gtgctgattg ccattctggt gtttatcatc accacgcagc
    23161 tggtgcgcaa tctgcccgcg ctgctggaac tggcgatttt gcagcacctg gatttaacgc
    23221 cgggtaccgg ctacgccatc accaccatca ccaaatatct gctgatgctg attggcgggc
    23281 tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt gcagtggctg gtcgccgcgc
    23341 tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc caactttatc tctggcctga
    23401 tcatcctgtt cgaaaaaccg attcgcattg gcgatacggt gacaattcgc gatcttactg
    23461 gtagcgtgac gaaaattaat acccgcgcaa ccaccatcag cgactgggac cgtaaagaga
    23521 taatcgtgcc gaacaaggcg tttattaccg agcagtttat caactggtcg ctctctgact
    23581 cggtcacgcg cgtggtgttg acgattccgg cccctgccga tgccaacagt gaagaagtaa
    23641 cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt gatcgacaac ccggcaccgg
    23701 aagtcttcct ggtggatctg caacagggga ttcagatttt cgagctgcgt atttacgccg
    23761 ctgagatggg tcaccgtatg ccgctacgcc atgagatcca ccagctgatt ctggctggct
    23821 tccatgccca cggtatcgat atgccattcc cgcccttcca gatgcgtctg gaaagcctca
    23881 acggtaaaca aacggggaga acgctgacgt ctgcgggcaa aggtcgtcag gcgggaagtt
    23941 tgtaatgcat gaagctgcca aatgatgtca acgtggctca tttagcagct taacatcatc
    24001 ttcagttatc accggccctg gagagtatca tccatacgca ttacttcctg acaactcatc
    24061 gccggagaga cgatgagttg caggatttca tatgatcagt cgagagctaa ctcaggttta
    24121 ttcgccatgc gactgcaata gttctggtaa atcgccatcg ctaacagcga gaagaagaca
    24181 gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg cttcaaccac aggctgaata
    24241 atggtgaaga cgttcgcaaa tgtcaccacc agaacgacaa ccaccgtcgc aatcattgcc
    24301 gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat cctgacgtgc tttaaagaat
    24361 gggaaagcca gcgcgaggaa caggtaagga agcgtcatag acacgttcgc catcagcgtc
    24421 agcttgttaa agaacgccga tgcggtaccg ccaccaaacg aaaccagcag gatgaagaca
    24481 gtaaccaacc cgcactgcat ccacatagcg atagacggca tccccatcgc attcaggcgc
    24541 gtcatcggtt ccggccacaa cgctttcggc gtcccctgga tgatggcttt cagcggtgaa
    24601 tagcaaagcg taaagaacgc accggtataa gcaaggaaca tcgaaagtcc ggtaatacgc
    24661 gcaaaccata cgcccagcga caatgacgct tcaggtgaca aatgcagtgc gttacccagc
    24721 gtcaccccga ggctcttcat cagcacatag gtaatattgc cgaggttaac ggaaccatta
    24781 cttaatacct gctgccagtt tgtgctgacg ccccataaaa atattgccag cgaataaccg
    24841 attgaaataa caatagcggc aaaaacaata cctttggcaa agttcttttc tggattttcc
    24901 gttttatcga ccagaccacc aaccgcttca attccgccat aggcaaaaat agcaaatacc
    24961 acaaacgata gcattgccag accggactga taacctgggt tcggtgatgc aaggaaatta
    25021 atatcctgcg cgaaatgccc accatttaat aacaaaatag taatgcttac taacagcaat
    25081 actaaattca gacacattac tgcaataccg cccaccgcag taatgcgggc aattttatta
    25141 atcccctttg aagcaacgac ggtgaccaga atcatccatg ccactgccag cagaccaacc
    25201 acctgcgtag gctccagtcc ggcaatacgc cagtgctggg tcatgtcgct accatagagg
    25261 aatgttgaga acggtaccca aactttcgcg gaggtgctca ccatccagat gatataagag
    25321 gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc cgacactatt attcatccag
    25381 gaatagatac cgccttcttc tttgcgataa gcagctccca tttcagccat cattaaggcg
    25441 aatggaataa agaataacaa tgcagaaaat atataaaagg gaatcgcact ataacccatt
    25501 aagtaataag ccgatgggct attggcaaat ccaaatacgg aagtaaagat catcaatatg
    25561 agtcctatca gactcatctt ttttatcgtg tgaggcatga aaccatcctt catttcagcg
    25621 cgtgtataaa acgcgcccac tgaactgcac tgcccaacat tgagcgggca gcgtaaaaac
    25681 caatacagaa ttgatacctg gatgatatca aatatccagt cttaaattgt ggcctttagc
    25741 ggaaaaatga aaacatatgc tacagaatgg cgcttatcac gccattcttc gctgttaatt
    25801 cagtaatttt tcagaattat gcccggtcaa cgctaaaggc gatgacttca gccagtgtct
    25861 ccgcgcccag cgccaacatc accagacgat caacacctaa tgccacgccg gaacagtcag
    25921 gcataccgac tttcaaggct tcaatcagat tctgatcaat ggggtgctgc ggcaaaccgc
    25981 gcgccgcgcg cttacggtta tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca
    26041 attcatggaa accattcgcc agctcaatac ctttataata aacctcaaag cgttcagcga
    26101 cccgatgatc ttcggtactg atttgcgcca gtgatgcctg gctggctgga aagtggtaca
    26161 caaaggtcgg tttttcttta ccaatatttg gttctacgcc aaaggtaaac agcaattgta
    26221 gcaacgtgtc gcggtcttct tcggtatcag caacattgct caaatccagt ttcgctgcca
    26281 cttcccgcag ttgcgttttg tcggcagaga gcgggtcaat ttccagataa cgcaagaaag
    26341 cttgttgata agaaaggctt tctgctgccg ggcagtccag cacctgttgt aagagatcgt
    26401 ccacctcgtt catcaaccgg tacatatcat agtgcggtcg ataccactca agcatagtga
    26461 actcagggtt gtgataacgc cccatctctt cattacggaa actgcggcac agctggaata
    26521 ccggcccgca accggcaacc agcaggcgtt tcatatggta ttccgggctg gtcattaacc
    26581 agagattcat cccctgcgaa tgcccggggc caacgaaacg tgtctcaaac gggaccaaat
    26641 gaatatcggt taccgtcgcc tggctcatac aaggcgtctc cacctccagc actccacgat
    26701 cggcaaagaa acgacggatc tccgccataa tcgccgcgcg ttttaataag ttaggaatgg
    26761 atgcgctcgg ctgccaggat gccgtttcgc tcatagttaa atctccagtt tttgacaagg
    26821 gcacgaagtc tactcgcaac gcgacggcga gacaaatttt acgcaggaat aaaacaacgg
    26881 tgggcagtga ctaaaaaaag cacgatctga tggtttagta attaaattaa tcatcttcag
    26941 tgataattta gccctcttgc gcactaaaaa aatcgatctc gtcaaatttc agacttatcc
    27001 atcagactat actgttgtac ctataaagga gcagtggaat agcgttcgca gaccgtaact
    27061 ttcaggcact taccctgaag tacggggctg tgggataaaa acaatctgga ggaatgtcgt
    27121 gcaaaccttt caagccgatc ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc
    27181 aattgctgcc gcgcaggcaa atccaaatgc aaaaatcgca ctaatctcaa aagtataccc
    27241 gatgcgtagc cataccgttg ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga
    27301 cagcttcgaa tatcactttc acgatacagt agcgggtggc gactggttgt gtgagcagga
    27361 tgtcgtggat tatttcgtcc accactgccc aaccgaaatg acccaactgg aactgtgggg
    27421 gtgcccatgg agccgtcgcc cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa
    27481 aatcgagcgt acctggttcg ccgccgataa gaccggcttc catatgctgc acacgctgtt
    27541 ccagacctct ctgcaattcc cgcagatcca gcgttttgac gaacatttcg tgctggatat
    27601 tctggttgat gatggtcatg ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct
    27661 ggtgcagatc cgtgctaacg cggtcgttat ggctactggc ggtgcgggtc gcgtttatcg
    27721 ttacaacacc aacggcggca tcgttaccgg tgacggtatg ggtatggcgc taagccacgg
    27781 cgttccgctg cgtgacatgg aattcgttca gtatcatcca accggtctgc caggttccgg
    27841 tatcctgatg accgaaggtt gccgcggtga aggcggtatt ctggtcaaca aaaatggcta
    27901 ccgttatctg caagattacg gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa
    27961 atatatggaa ctgggtccac gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa
    28021 aggcaacacc atctccacgc cgcgtggtga tgtggtttat ctcgacctgc gtcacctcgg
    28081 cgagaaaaaa ctgcatgaac gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg
    28141 cgtcgatccg gttaaagaac cgattccggt acgtccgacc gcacactaca ccatgggcgg
    28201 tatcgaaacc gatcagaact gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg
    28261 ttcctctgtt ggtctgcacg gtgcaaaccg tctgggctcc aactccctgg cggaactggt
    28321 ggtcttcggc cgtctggccg gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg
    28381 caacgaagcg gcaattgaag cgcaggcagc tggcgttgaa caacgtctga aagatctggt
    28441 taaccaggat ggcggcgaaa actgggcgaa gatccgcgac gaaatgggcc tggctatgga
    28501 agaaggttgc ggtatctacc gtacgccgga actgatgcag aaaaccatcg acaagctggc
    28561 agagctgcag gaacgcttca agcgcgtgcg catcaccgac acttccagcg tgttcaacac
    28621 cgacctgctc tacaccattg aactgggcca cggtctgaac gttgctgaat gtatggcgca
    28681 ctccgcaatg gcacgtaaag agtcccgcgg cgcacaccag cgtctggacg aaggttgcac
    28741 caagcgtgac gacgtcaact tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac
    28801 gactcgcctg gagtacagcg acgtgaagat tactacgcta ccgccagcta aacgcgttta
    28861 cggtggcgaa gcggatgcag ccgataaggc ggaagcagcc aataagaagg agaaggcgaa
    28921 tggctgagat gaaaaacctg aaaattgagg tggtgcgcta taacccggaa gtcgataccg
    28981 caccgcatag cgcattctat gaagtgcctt atgacgcaac tacctcatta ctggatgcgc
    29041 tgggctacat caaagacaac ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg
    29101 cgatttgtgg ttcctgcggc atgatggtta acaacgtgcc aaaactggca tgtaaaacct
    29161 tcctgcgtga ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac
    29221 gcgatctggt ggtcgatatg acccacttca tcgaaagtct ggaagcgatc aaaccgtaca
    29281 tcatcggcaa ctcccgcacc gcggatcagg gtactaacat ccagaccccg acgcagatgg
    29341 cgaagtatca ccagttctcc ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc
    29401 agtttggcct gaacccagag ttcatcggtc cggctgccat tacgctggcg catcgttata
    29461 acgaagatag ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg
    29521 gcgtatggag ctgtactttc gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc
    29581 cggctgcggc cattcaacag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc
    29641 tgaaaccacg ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg
    29701 acgtccacct ggtggaaaaa attgccgttt tatcgctttt acatgctgcg cgaaggcacg
    29761 gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat
    29821 ggcccggaag cctgggcggg attcgtcgac tttttacaaa acccggttat tgtgatcatt
    29881 aacctgatca ctctggcggc agctctgctg cacaccaaaa cctggtttga actggcaccg
    29941 aaagcggcca atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt
    30001 ctctgggcgg taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa
    30061 ggagcctgag atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct
    30121 cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg
    30181 tattctgctg ccgctggggc tgtttccggg tgatgcactg agctacgagc gcgttctggc
    30241 gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg
    30301 gtgtggttta caccgtatgc accacgcgat gcacgatctg aaaatccacg tacctgcggg
    30361 caaatgggtt ttctacggtc tggctgctat cctgacagtt gtcacgctga ttggtgtcgt
    30421 tacaatctaa cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg
    30481 aaaccagacc ctatgttcaa aacgacgctc tgcgccttat taattaccgc ctcttgctcc
    30541 acatttgctg cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata
    30601 gagcaacaaa agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac
    30661 tttacctggg ggtatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt
    30721 gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga
    30781 ggggaaatca agttaagcga tcccgcaaca aaatactggc ctgaacttac cgctaaacag
    30841 tggaatggga tcacactatt acatctcgca acctacactg ctggcggcct gccattgcag
    30901 gtgccggatg aagtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct
    30961 gcatgggctc caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca
    31021 ctggctgtga agccgtctgg tttgagtttt gagcaggcga tgcaaactcg tgtcttccag
    31081 ccactcaaac tcaaccatac gtggattaat gtaccgcccg cagaagaaaa gaattacgcc
    31141 tggggatatc gcgaaggcaa ggcagttcat gtttcgccag gggcgttaga tgctgaaact
    31201 tatggtgtga agtcgaccat tgaagatatg gcctgctggg tacgaagcaa tatgaatccc
    31261 cgtgatatca acgacaaaac acttcagcaa gggatacaac tggcacaatc tcgctactgg
    31321 caaaccggcg atatgtatca gggtctgggc tgggaaatgc tggactggcc ggtaaatcct
    31381 gacatcatcg ttaacggcag tgacaataaa attgcactgg cagcacgccc cgtaaaagcg
    31441 attacgcctc caactcctgc agtacgagca tcatgggtgc ataaaacggg ggcgaccggc
    31501 ggatttggta gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct
    31561 aacaaaaact atcccaatcc agcgagagtc gccgccgcct ggcagattct caacgctcta
    31621 cagtaaaatt tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat
    31681 ctacacttag aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca
    31741 gcgacagctg catttctggt cgttgcctgc agttctccta cgccgccgcg tggcgtgacc
    31801 gtagtgaata atttcgacgc taaacgctat ctcggtacct ggtatgagat tgcccgtttt
    31861 gatcaccgct ttgaacgtgg actggaaaaa gtcaccgcaa catacagcct gcgtgatgac
    31921 ggcggcctga atgtcattaa taaaggctat aaccctgaca gaggaatgtg gcagcagagt
    31981 gaagggaaag cgtactttac cggcgcacca actcgcgctg cgctgaaagt gtcattcttt
    32041 ggtcctttct atggcggtta taacgttatt gcactcgatc gggaataccg ccatgcgctg
    32101 gtttgcggac cggaccgcga ctacctgtgg atactctccc gcacgccaac catttctgac
    32161 gaagtgaaac aggagatgct ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt
    32221 atttgggtac agcagcccgg tagttagtga gtgctgagtt tcagaccaat aatccccaat
    32281 acgattaacg ccagactcgc cagacgcatc gggttagcgg actcaccgag cagcacaatg
    32341 ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcataagc cgtccctact
    32401 ggtaacgatt tcatcgccca ggcaagtagc gccatactga caatcatcgc cgtcacggta
    32461 ataacactcg gcgtcaaacg actaaagccg tgggtatatt tcaggcctac ggcccatacc
    32521 acttccagca gaccagcaat aactaagata atccaggaca tatcaggctc cggagaattg
    32581 gggccgtccc cggtgaaaag acgcgtttgc aggtcgtcct gcaaagctga tggttgaacg
    32641 gatattttgc caggtggcgg gggaatttca actattacct ttgtgactat cgcagaaaaa
    32701 acaaacggca cgacacagct gtcgtaccgt tattgcttat tgctgcgctt tcgttgctgc
    32761 accagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg tggtgttgca
    32821 ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg tcttcttcac
    32881 cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat taataataga
    32941 caacatcgcc aaaaatgatg gaaatctgat gatttttcgg aagagaagaa aaattagctg
    33001 gcagcgcggg agatggagtt gccgagatgt ttgatgtctt cgccgaaacc gcgagcggtg
    33061 ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg tttcatcatc
    33121 tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca ttacttcacg
    33181 cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat ttgtacgaac
    33241 agcggaactt taaccacagc gccagtagac agggtagccg gtttgccgcc agtacctgcg
    33301 gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa gttcggcgga
    33361 gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg atccagcagc
    33421 catttagcgt tgtcgccaat tgcttttgcg tcagcagaca gctgctcgaa agtttcgttg
    33481 ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat atcgacaaca
    33541 tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt accggtcagc
    33601 agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac gaattcactc
    33661 gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg aaaatcgttg
    33721 ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa gccacaaaat
    33781 ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc aacttgccga
    33841 tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg acgaaaaact
    33901 gttggccgga cgcaacgcca aaaagctgtt tgccctgcgt gtgccccgct catttatcga
    33961 tcgcatggag aaaggcaatc cggacgatcc gcttttgcgt caggtactta cctcgcaaga
    34021 tgagtttgtc gtcgcgcccg gattctccac cgacccgctg gaagaacagc acagcgtagt
    34081 gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag gcggctgcgc
    34141 ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc agggcaacaa
    34201 gcgtaactgg caaactgcac ttgagtatgt tgctgcgcat ccggaactgg acgagatgat
    34261 tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt tgctcacaca
    34321 actggaagcc atcccgcata tcaaacgtct gcggattcac agtcgtctgc cgattgtgat
    34381 cccggcacgc atcacagatg cgctggttga acgcttttca cattctacgc tgcaaatctt
    34441 gctggtgaat cacatcaacc atgccaatga gatagatgaa acattccgtc aggcgatggc
    34501 taagttgcgc cgggcgggcg ttactttgct gaaccagagc gttctgttac gtggtgtgaa
    34561 cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgcag gcgtgatgcc
    34621 gtattacctg catgtgctcg ataaagtaca gggcgcggcg cattttatgg tgagtgatga
    34681 cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggatatc tggtgccgaa
    34741 actggcgcga gaaattggcg gcgaacccag caaaacgccg ctggatctcc agctacgcca
    34801 gcagtaactg attaaacagg gaagtttaat ctttgcccgt tacataacct ttaattattc
    34861 atatacat
//
LOCUS       DAZFKY010000028        34660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFKY010000028 DAZFKY010000000
VERSION     DAZFKY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(98..1351)
                     /gene="amiC"
                     /locus_tag="ACHKCL_001244"
     CDS             complement(98..1351)
                     /gene="amiC"
                     /locus_tag="ACHKCL_001244"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5762250.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1583..2914
                     /gene="argA"
                     /locus_tag="ACHKCL_001245"
     CDS             1583..2914
                     /gene="argA"
                     /locus_tag="ACHKCL_001245"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5762251.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2976..4802)
                     /gene="recD"
                     /locus_tag="ACHKCL_001246"
     CDS             complement(2976..4802)
                     /gene="recD"
                     /locus_tag="ACHKCL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5762252.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4802..8344)
                     /gene="recB"
                     /locus_tag="ACHKCL_001247"
     CDS             complement(4802..8344)
                     /gene="recB"
                     /locus_tag="ACHKCL_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5762253.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8337..11225)
                     /gene="ptrA"
                     /locus_tag="ACHKCL_001248"
     CDS             complement(8337..11225)
                     /gene="ptrA"
                     /locus_tag="ACHKCL_001248"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5762254.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11401..14769)
                     /gene="recC"
                     /locus_tag="ACHKCL_001249"
     CDS             complement(11401..14769)
                     /gene="recC"
                     /locus_tag="ACHKCL_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5762255.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDHELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14782..15105)
                     /locus_tag="ACHKCL_001250"
     CDS             complement(14782..15105)
                     /locus_tag="ACHKCL_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5762256.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(15090..15497)
                     /locus_tag="ACHKCL_001251"
     CDS             complement(15090..15497)
                     /locus_tag="ACHKCL_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5762257.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15494..16057)
                     /locus_tag="ACHKCL_001252"
     CDS             complement(15494..16057)
                     /locus_tag="ACHKCL_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5762258.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16048..16518)
                     /locus_tag="ACHKCL_001253"
     CDS             complement(16048..16518)
                     /locus_tag="ACHKCL_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5762259.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16702..17496)
                     /gene="thyA"
                     /locus_tag="ACHKCL_001254"
     CDS             complement(16702..17496)
                     /gene="thyA"
                     /locus_tag="ACHKCL_001254"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5762260.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17503..18378)
                     /gene="lgt"
                     /locus_tag="ACHKCL_001255"
     CDS             complement(17503..18378)
                     /gene="lgt"
                     /locus_tag="ACHKCL_001255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5762261.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18529..20775)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_001256"
     CDS             complement(18529..20775)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_001256"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5762262.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20788..21318)
                     /gene="rppH"
                     /locus_tag="ACHKCL_001257"
     CDS             complement(20788..21318)
                     /gene="rppH"
                     /locus_tag="ACHKCL_001257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5762263.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21671..21817)
                     /gene="ygdT"
                     /locus_tag="ACHKCL_001258"
     CDS             complement(21671..21817)
                     /gene="ygdT"
                     /locus_tag="ACHKCL_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5762264.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22003..22692
                     /gene="mutH"
                     /locus_tag="ACHKCL_001259"
     CDS             22003..22692
                     /gene="mutH"
                     /locus_tag="ACHKCL_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5762265.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22761..23474
                     /locus_tag="ACHKCL_001260"
     CDS             22761..23474
                     /locus_tag="ACHKCL_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5762266.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23612..23830
                     /gene="ygdR"
                     /locus_tag="ACHKCL_001261"
     CDS             23612..23830
                     /gene="ygdR"
                     /locus_tag="ACHKCL_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5762267.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23938..24978
                     /locus_tag="ACHKCL_001262"
     CDS             23938..24978
                     /locus_tag="ACHKCL_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5762268.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25010..26203)
                     /gene="lplT"
                     /locus_tag="ACHKCL_001263"
     CDS             complement(25010..26203)
                     /gene="lplT"
                     /locus_tag="ACHKCL_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5762269.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26196..28355)
                     /gene="aas"
                     /locus_tag="ACHKCL_001264"
     CDS             complement(26196..28355)
                     /gene="aas"
                     /locus_tag="ACHKCL_001264"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5762270.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            28941..29972
                     /gene="galR"
                     /locus_tag="ACHKCL_001265"
     CDS             28941..29972
                     /gene="galR"
                     /locus_tag="ACHKCL_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5762271.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29979..31241)
                     /gene="lysA"
                     /locus_tag="ACHKCL_001266"
     CDS             complement(29979..31241)
                     /gene="lysA"
                     /locus_tag="ACHKCL_001266"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5762272.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31363..32298
                     /gene="lysR"
                     /locus_tag="ACHKCL_001267"
     CDS             31363..32298
                     /gene="lysR"
                     /locus_tag="ACHKCL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5762273.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32285..32977)
                     /gene="ygeA"
                     /locus_tag="ACHKCL_001268"
     CDS             complement(32285..32977)
                     /gene="ygeA"
                     /locus_tag="ACHKCL_001268"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5762274.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33106..34524)
                     /gene="araE"
                     /locus_tag="ACHKCL_001269"
     CDS             complement(33106..34524)
                     /gene="araE"
                     /locus_tag="ACHKCL_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5762275.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8490 a         9530 c         8560 g         8080 t
ORIGIN      
        1 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
       61 ggtctcctat aaaaaagcgc cattcagcgc ctttttatca tccccttctc gccagggtcg
      121 ccccatcggc aaaatacgct ttaattcccg caagaataga ctccgcaact tcctgctgga
      181 aggtcgccgt tttcagttta cgctcttcct caacgttact gataaacgcc gtttcgacca
      241 gaatggaggg aatatctggt gcctttagta cggcaaaccc ggcctgttca acttgatttt
      301 tatgcagctt gttgatttta ccgagcttat tcagcaccgc tttaccaaac ttaaggctgt
      361 cagcaatggt cagcgactgc accatatcga acatggtgtg gtcgacatag cggtcaccgc
      421 ttttgctcac gccaccaatc aagtcggagg cgttctgggt ttgtgccaga tattttgccg
      481 cagtactggt tgcacctttg gttgagagcg caaacaccga ggaaccgctc ggctggcgac
      541 tggtaaaggc gtcggcatgg atagagacaa acaggtcagc acgctgtttc tgagcttttg
      601 ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac accttcatat
      661 tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat ttgcaatacc acatcttttt
      721 cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc accgtggcca gggtcaagca
      781 taatgacaat cggacgatcg cgccctgctt tacctggttg tggaccactt tgcgctggcg
      841 gcacctgctt ttcgaggtcg cctttgttgt aatcctccag cagcgccagc agcgggtcct
      901 gcatatcctg tgcattggcc ggatagaggt ccatcaccag acgctcttta aacccggcga
      961 ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt taattcaaaa accatccgta
     1021 cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa
     1081 tttgtgccgc catccccttg agtaccgagt tcaggtttac atcttcgata tccaccacca
     1141 cgcgttccgg gttactcaac gcgaactgct tatatttcag ctgacgattt gattctaccg
     1201 tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc cacgacctgg ctgaccgcag
     1261 ccagactgac ctgacttacg ctcaatagcc acatggcacc cgcgccttgc agtaaacgac
     1321 gacggctgat tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga aatctaagct
     1381 gtgtaacaag taaacgacta atttgaccgg aaactttagc gaaagacgca taatctgtca
     1441 tctaataaac ggtaaacatt ctttttatat tcacgccatt actgataaaa aagtcgctct
     1501 cgcataaaat ttacacttgc accctgcgaa aaaacagaat aaaaatacaa taaattcgaa
     1561 taataatgca aagaggtgtg ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt
     1621 ccgccattcg gttccctata tcaataccca ccggggaaaa acgtttgtca tcatgctcgg
     1681 cggtgaagcc attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca
     1741 cagcctcggc atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct
     1801 ggctgcgcat caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac
     1861 actggaactg gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc
     1921 gatgagtctc aataacacgc cgctacaggg cgcgcatatc aacgtcgtca gtggcaattt
     1981 tattattgcc cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat
     2041 ccggcggatt gatgaagacg cgatccatcg tcaactggac agcggtgcaa tagtgctaat
     2101 ggggccggtc gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc
     2161 cactcaactg gccatcaaac tgaaagctga gaagatgatt ggtttttgct cttcccaggg
     2221 cgtcactaat gacgacggtg atattgtctc cgaacttttc cctaacgaag cgcaagcgcg
     2281 ggtagaagcc caggaagaga aaggcgatta caactccggt acggtgcgct ttttgcgtgg
     2341 cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga
     2401 tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga
     2461 aagcgccgag cagattcgtc gcgcaacaat caacgatatc ggcggtattc tggagttgat
     2521 tcgcccgctg gagcagcaag gtattctggt acgccgttct cgcgagcagc tggagatgga
     2581 aatcgacaaa ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta
     2641 tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg
     2701 cagctcatca cggggcgagg ttctgctgga acgcattgcc gctcaggcga agcagagcgg
     2761 cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac tggttccagg aacgtggatt
     2821 taccccggtg gatattgatt tattgcccga aagcaaaaag cagttgtaca actaccagcg
     2881 taaatccaaa gtgttgatgg cggatttagg gtaagcgatg aaaatcgtcg gatgcgacat
     2941 gcgtaacact cgtacgtcgc atccggcaat tacgtttatt cccgtgaact aaacaacgcc
     3001 gccagaccac tgcgccgctc agtacgagtg gcgattgccg cacttaatat gcgctcatcg
     3061 gcatacagcg acagacggcg acgcgcgcgg gtcaccgcgg tataaaccag ctctcgcgtt
     3121 actaccggcg tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc
     3181 gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca
     3241 gacttaatat tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc gcgatccagc
     3301 gcaataccga tatcgccatt aaacaaccca agcgcgctgt cattacgggc aatcatcacc
     3361 ggtcggcctt cgtaccaacg agagtgcgga ttacgatgaa ttttgcgctt ctgttgcata
     3421 aactgctcaa ttcgctcatt cagtccagcc acgccaaatg gcccttcccg cagggcgcac
     3481 aaaagttggt actcattgaa cgcctgaatg attaaatccg gctcggcacg cgcttgcagc
     3541 agatccagat aacgtccata acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg
     3601 ccgctctgta aaagccgttt ttcaatatca gtaaaatcct gctgaaaaac ggttttcact
     3661 gccgttttat caccacggtt gatcgccgca gctaactgac caatgccaga atcgctgccg
     3721 aaacgatagc ttttttgcag caggcagaga ctgtcgcgca aagatgccgc ttctgtgcca
     3781 gttcctgccg gaacgtgagt tcccgtcagg cggcttagct gcccggcacg ctcggcggta
     3841 aagccggcat tggcataagc gcagatatcg cccagcacag ccccagcctc aaccgaggcc
     3901 agttgatcac gatcgccgag aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt
     3961 ctcgacatca taggcagatc gatcattgac gcttcatcta ccaccagcac atcaagatgc
     4021 agcgggttac cggcatgatg acgtaaacgc tggctacccg gctgcgcacc cagcaatcgg
     4081 tgcaaagtac tggcatcttc cggaatacgt ttcttttgtt catcggtcag cggtaactgt
     4141 cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc
     4201 agacggatac ggcagcgttc gccgtcggcc atttgaatta acgctgccag caacttcgct
     4261 acggtggtcg ttttaccggt gccagggccg ccggaaatca ccgagatccg ccgcgtcagc
     4321 gccactgctg ccgccacttt ttgccagtta atttcatcac cagtcggaaa gagtttatcc
     4381 agggtttgcg ccaacaaggc ttcatccacc tcgatggcat gattcacctc attgaagaag
     4441 cgcgctaccg tccgctcgtt acaccacatg cgattcaaat aaagacgatc gccacagagg
     4501 atcatcggcg tgggttcatc tccccggctg accgcttgtg aagccagcaa gcattcttcc
     4561 caattttgta gctcaccgat ttcactgaca caggtcgcca acagcggatg cgactcctca
     4621 ttattttcca gtcgtgaaag cggcaaacaa acgtgcccct ctccggcatc atgacttaac
     4681 agtgccgccg cgagggtgac ggcaggatgt tcatctcccg ccacggtcag ggcaaactgc
     4741 acgtccagcg ggcgtagctg tttgtgctcc acagcttcca gtaattgctt ttgcaatttc
     4801 attacgcctc ctccagagtc ataccggcaa acatctcatc catcaggtca atcaacccgg
     4861 cgttggggcg ggttgtgtaa atcccttgtt gcggatgttc tttatcaaca ccacgcagga
     4921 acagataaat aacgccgcca aagtggcgct catagtcgta atcagcaatg cgatggcgca
     4981 gataacggtg cagcgccagg gtataaagct gatattgcag atcatagcgg tgtgcctgca
     5041 ttgccgctgc catagcctgt tgggtgtaag ccgaactatc ttcacccaac cagttggatt
     5101 tatagtcgag caggtaataa cgcccttcgt ggcggaacac caggtcgata aagcctttta
     5161 acatgccacg tgcctgcatg aactccagcg gcgggcagcc agcggatagc gggtcaaact
     5221 ggcgaattag tgcatcaagc tggctggcga tgagcggttc gctaatcggc aggtaaaact
     5281 ccatctccac ctgtttatcg cggtcggaaa gctgactcag gctaacaccc gtttcattga
     5341 gaggtgcctg gaggacagcc gtgatccact cggtcaacac cggttcccac tgcgattcaa
     5401 agccgccgag ttccagtttt tcctgtaccc agttcgggtc aaccggctgg gtaaaattga
     5461 ggtcttcaaa caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg
     5521 gtgttaacgt cggttcttca acgacgctga ccacgcctgc ggcatcaaca tccagccgtg
     5581 gcatcagatc ctgagcgata ccgtgaccac gctgctgcaa gccggagtag ctggtgacac
     5641 gccagttatc gccgggcaat cgttgtagcg tcctcgcatt cagttctgca gttaaagcat
     5701 cattaacctg ccagggctgg ttatcaccag tttgtgccgt ttgccaggca atatcatcat
     5761 cgcataacgc ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca
     5821 gcaaacgccc gagggcactt tggtggacgt cggtgtcacc ttttttatcg ccacgacggc
     5881 gcaccagcgg tgcaacgccg agactgcaat gccaaaccga acgcgtcagc gccacgtaaa
     5941 gcaaacgcag atcttccgcc agacgttcga cctccgcaag atcgacgctt tctggcgcag
     6001 cgttaagatc cagaacagcc tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga
     6061 cgcggaaatt ggtaataaat ggcaaccaga ccagcggata ttccagccct tttgatttat
     6121 ggatcgtgac aatctgtacc agatgtttat cactttcgag gcgcatttgt tggctggagg
     6181 cattactgtc tggctcgagg atatgttgcg ataaccagcg taccagcgca tgttcacttt
     6241 ccagctgcgt tccggcttct tgtagcagtt cgctgatatg caagatatcg gtaagacgcc
     6301 gctcaccgcc tgccgttgcc agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc
     6361 gcatcatcgg cataacgcca cgtttgcgcc agatttgccg ataaccatcg aactcttcga
     6421 ccaccgcgtc ccacgcatgt tcgtcattat tcagcgtttc gatatccagc gcgttcagcc
     6481 ccatcattga cgttgccagc gcactacgca gggtgttctc acgttcgggc gtcatcaccg
     6541 cctgcaacaa ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg tcgcggttcg
     6601 aaaggtaaac ggaagggatt tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct
     6661 ggcggctgcg caccagcaca ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt
     6721 tcatcagcaa cgcttcgccc cgctgtccgg cttgtagcca gtcgcgaatt tgcgcagcac
     6781 ttacctgcgc catggtactt tgatagtcgc caacgccgca actttcgcct tccatcagcc
     6841 acattttcat cgcaggctgc gtttcacctt taaagacaaa acgtaacgcc tgatttttcc
     6901 cggctgattt cactggaata aacggtattt cgcgaaacat gaacgcgtca tcagtctggc
     6961 tgaaaagctt gttcacactg ttcaccattc caggtgcgga gcgccagttg gtgtctaaag
     7021 tgtagtgggc gtgaacttca ctacgcgcct tcatataagt gaagatatcc gcaccccgga
     7081 atgcatatat ggcctgcttc gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt
     7141 gccagatacg gcgaaaaatt cggtactgct gggggtcggt atcctgaaat tcatcgatca
     7201 ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac ctcaccgctt tcgctacgca
     7261 gcgcggaatc gagccgactt aacatgtcat caaaacccaa ttcgccacgg cggcgttttt
     7321 cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt gatcaccaga tcgcggatcg
     7381 acaatggttc tgcaagcagt tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc
     7441 ccccggcctt cgtgcgatct tctaagaaac gctgggagaa tttttccagc gactccggca
     7501 actgataact gtttctctct tcttctgccc aggcgctgat cttgtcgatc catttagcct
     7561 gattgctacg gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt
     7621 cacccactgc gtcgcgccac tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt
     7681 gacgggaagc cagcgtttca tcatcgggcg gcggtgcttt gataaccggc gcttcacctt
     7741 gcagataacg attaatatcg cgcagcaacg cctgcggccc tttccaggtt tcaaagacga
     7801 cctgggctat ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct
     7861 ggtaacgtag cagagactca tcttcaatca gctgctgctc aaacagcatg ccggattcaa
     7921 aggcattcag gttgagcatg cgctggcaaa aaccgtgaat agtaaagact gccgcttcat
     7981 ccatctgccg ttcggctaac aacaaccact tcgcggcttg cgctttatcg tcgatctctt
     8041 ccagcagacg ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg atgcgcaact
     8101 catggatatt gctacggata cgaccacgca attctgccgt ggcagcctcg gtaaaggtga
     8161 ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa
     8221 gtaacaggcg caaatagagc gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt
     8281 caatcaggcg ctcgccctga aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac
     8341 tcattctttt cactcatcag gggcattgtt tgctgcaacg cgctgacgtt ctcccacact
     8401 ttccagcctt caggatgcac atattcggct ttcccgttct ggctgccgga aatctgcgac
     8461 agaatagcca tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc aagtttttgc
     8521 ggcgtcagca gttttatctg ggccacgatt ttatcacgcg aatcgaagcg catattgccg
     8581 cgatcgaaat ctttacttaa cttcaatgct tcttcgccga gcgtttgcgg tgcctgcagc
     8641 atctgggtaa ttaccgcctg ctggatttgc gcaaactcat ctggcttcat cgctcgcaat
     8701 tttgcctctg cggttgggaa aaacgccttg taacgctccc acaagaatga aggctgttta
     8761 tcattgcttt gcaaaaggaa gcccatgccc cactgacgcc ccacgctcat tggaaacgca
     8821 aacacggcat agcccaattg ttcttcggta cgcaactgat tgtagaacca cggctgtacg
     8881 atctgcccca acagagagct ataggctgag cttgtgtatt catcgtagcc agtcggtaca
     8941 aataccgctg ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat gacggattgt
     9001 tttttatcga ccactacatc tttgtttcga caccactctg aaccatcagc gcccaactgt
     9061 ttttgcacat cgcgtgccag cgttgttgcc tgggcctcgg tcatgttgcc gataaccata
     9121 aactctggtc gagcccctga ttttaaggcg tcacgataag ccagcacctc tttcaacgta
     9181 atggagggca aaattttacg ccgttcatct cgcgagaagt acggcacttg cgagagcatc
     9241 tgcgcgggca taatcgcctg ctcaaacgct ttaccctttt ctgcggaatc catcatctgg
     9301 ttataccagg acttcgcctg ctcaagctga tcttccgtag cggtatagct aaagtatccc
     9361 tcgagcaatg cctggaacag ctgcggcaga cgctgggtgt aaccattagc attaaccata
     9421 aggccgttgt tagcgttggt ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac
     9481 tgatcaagcg ccagccctgc gagataatca ttgagcgcaa acatcacctg attgcgggcg
     9541 ctgtccatgg ctttcggatt acgcaaaatc aggctgacat cagctttggg ttcgctggaa
     9601 aaataacggc ttggcgcata caccacgcgc agattcgact catcaacaat cagctctgga
     9661 tggtcatatt tcttctctga cttaatcagc gagaaatcgt caggaatata ggggttaagc
     9721 tccggtaagg agagcgcaat attggcagct ttttgctgcc agtcagcgaa agtttgttcg
     9781 ctgattttat cgacctgata cggcgcatcg acaaaataag ccgttttatt gtgcggctct
     9841 ttcgggctga tataccagat acgcgcattc tgcggcgtca tcatcgccag acgttctttt
     9901 actgctttag catcgtaccg atcggcaata ttgactgcat ccagcgtatg ctcaacagga
     9961 acgcgaatca tggtatctgc cagccattcg acgtaatcca tatcacgggt gattgacgga
    10021 taacggaagt cgatatccag cacattcgcc agttcatcga agtattgttt atcgatccct
    10081 ttttcacgta acaaattgag gtagctaaaa atagccgcca caacctgatc gcgattcgcc
    10141 agacctttat cggttaaaga cgcagagatc gctaatacgc cgctgttgcc gttgacgata
    10201 ggatcggagt tggcgctaat gccctcaact aacccctgct tttgcagcca gtcagacagt
    10261 gtacctgggc tgcgattacc aatcagatag gtaatcaatt catccgtttt actgcggaac
    10321 ttcgctgagt tgttatcgat gcgaaactca acgcgcagaa ctttacgcgg cagcgctggg
    10381 acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta ccggcacggt gatttccggt
    10441 tttttgctct ctttgttcgg cacgcgacca aaagtgtccg ccgccatttt tgccaactcc
    10501 ggcagcggtt tattactgta aataaccgcc ttcatcaaat tggcggaata gtacttctcg
    10561 tggaaatctt tcagcgcctg ctgcaccgga ttaccaggtt tgtcgcttaa agtttcgagg
    10621 ttaccaccag aaaactttga accggggtgt gccgggttaa tggtttctgc gctgacctgt
    10681 gccatgcgca tcccgtcacg tgtacgcgcc atggttaatt cagcgttcac tgcattacgc
    10741 tcacgttcgg cgtatttctt gtcgagcaag ggttctgcaa tagcatcggc caggcggtct
    10801 accgcaccgg gcaaggcgtc gttctcaact tccagataga aagccgtgcg atacggcgca
    10861 gtgctggcat tgtgactacc gccgtgcatt ttgagatatt cggccagact gtcagcctgc
    10921 gggtactttt tcgaccccat cagactcata tgttcaaggt aatgtgccag cccctggtac
    10981 gcctcgggat cttccagcga cccaacgggc accaccagcg ccgatagcga tttaactgcc
    11041 tgcggatcag aaaccagcaa gaccaccata ccgttatcca gacgtatagc ctgatactgg
    11101 cggttatctt tatcactttt acggatggtt tcctgaatcg gctgccatcc cgtttctgcc
    11161 tgacttaagg gtgcccaaag ggcaactaac aacaataatg ctttgaacca ggtgctgcgg
    11221 ggcattcacg gacctcataa tcaactttat tttctggcca gattcaacag gttaacgcaa
    11281 atcatctgcc agaatttaat cttgtgctgc acgagtcagc ctatgtttat ataaccatca
    11341 gtccgtgact ggtgcgcatc ataaagtaag cggatagatt gcgcaatttt tatacagcgc
    11401 tcatgactga ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt caacgattgc
    11461 ctccattgtc tctggtgtta attgccgcca gagcctttga taccagatat catcaccttc
    11521 gccactcacc atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa
    11581 cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc
    11641 gccgccactt tcaggtaaca ccagcaatgg cgcggacatc ccttcacgat acccctcaat
    11701 cagttgtgag aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc
    11761 gtctttgcgt agaaaaaggc gactttcacc attaccaccg ctggcacagt agacaaggtg
    11821 ttccagccaa agttgcattc cctgcgccac acttaataaa gagggacgcc agcgcaacaa
    11881 gccatccggc tgcacctgcg gcaaccagcc agttatctgc acaccgttgc aagcgagatc
    11941 aatttccata ctctgccccg gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat
    12001 ctcctggcac tgtgtttccc agaaaatttc accaaaagcg ccatacggta aatcccctgc
    12061 cgcccggaag cggcggaaca agcgttcggc atcatcctgc tcaaccagtg cattcaataa
    12121 ctgctgattg atttgataac ggctaagtcc ttccagaata aatggctcgg tgtcggggat
    12181 ttcgctgtct tcagtacgga agttcacctg caaacgcatc tggaagaacg cccgcaccgg
    12241 atgtgcccag aatcgttgta gcgtttccag cggcacggtt tccggtaagg taaacggcag
    12301 cggctgaaca aattcagaat gtgctttacc agcctggctg gccgcaggta gccattcacg
    12361 agcatagctt tgtcgttcgc ctggctggta gttttgcgga tcaaacggca tccgggtatg
    12421 gaggcaagta agatgcgctt ttacccttgc ctcgctttca tcacagttga gcgcttcatc
    12481 gcccggtaga taatgacttt gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg
    12541 gaaacgttca ctgttatcct gaatggaacg cccgatatag ctgatataga gtttttgctg
    12601 cgcggaaatt aacgcttcca ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc
    12661 acgcttcggt ttctggctca tcaggtcaaa gcccaatggc gcaagctgac gtggataaac
    12721 gccgtcgttc attcccagca ggcaaaccac tttgaacgga attgaacgca ttggcatcag
    12781 agtacaaatg ttaaccggcc cggcgagaaa acgctggctg atacgttctt gatccaggcg
    12841 ctgtgccagt tcatcacgca atagtgacag cggcaccgca tcgccatact gcgcacctaa
    12901 accttcggcg ataatcgcct gccattgttg ttcgatcagc gtcatcgccg cttcggtttc
    12961 cgcatccggc aggaagaggg cgttgagcat atcgcgacaa accggcaacc actcttccag
    13021 cggacgctcc tgtgccagcc cgcgacgcca gatgtttagc tgcattagca gtgaagccag
    13081 atgccccacc agttctgcaa ttaagccgct cgattcatca taaggtagaa ccgattgcca
    13141 ctcgccctgc gcgctctcca tcgcgtagcc caacaacata cgcgtcaggc caaatcgcca
    13201 ggtgtgttgt ccggtggcgg ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca
    13261 acgaatgccg gattcgttga cccactgacg taaataacgc agcccttctt cggtgatgtc
    13321 aaaccgcgcc gccagcaccg gcacatccag taatgccagc acgtcttccg acacaaagcg
    13381 gctgtctggc aacgataaca ggctgataaa cgcttcaagt acaggatgcg actgccgcgc
    13441 ccgacggtcg gaaatggcgt aaggcaggta acgatccgca ggtgcgctac caaacacagc
    13501 ctgaataaac ggactgtagc tgtcgatatc agccaccatc acgatgatgt cgcgcggagt
    13561 aagtgtcggg tcttcttcca gcatcgccag caggcgatcg tgtaaaactt caacttcacg
    13621 ctgcgggcta tggcaaacgt ggaaggtgat actgctatcc agtggatcaa gcggacgttt
    13681 gttatcgcta cgggaaaact cttcgatgtt cacaccagca acggcgcggt tttccagttc
    13741 cagaatgtca gactgaatgt tatgcagcag gttatctggc gtcacatcga caaaggcgtc
    13801 cagctcctgg ctgctctcca ggtcagaaag gagataaatg tagtcgcgcc caagcttgcc
    13861 ccatgaggcc agcagcgggt tgccgacatc ctgttcacca tcgctgttaa agagctgccc
    13921 ggcattttcg ctgtcgcgaa atagcggtaa ttcgtgatct tcaaaactgt gtcggcgctg
    13981 gcgagtcagt agtttcgcca gataagcagg atctttaatg tcgccccagt aataacggca
    14041 ggggttggta aacaggagat ggatttcaat atgtttaccc agcgcctgga gcgcctggag
    14101 ataaacaggc ggtaacgcgg aaataccgca tataaagacg cgcgaaggta accccggcgg
    14161 gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga tagagattgg cgcggtgcca
    14221 gcgcggttgt ccgagttcat gggtatattc caccagcgcc ttccacaacg gcgcttgcca
    14281 ggcctgtgcc tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc
    14341 cggacgatag accagatact ggtcaaacag gtccgccgct tttgaggaaa gctggaacag
    14401 ctttcgcttg tcgctatcgt cagtcagata atgccgcaac agggtaaagt cttcgcgctc
    14461 cagcaattgc ggcagcagag tcatcagttt ccagctcatg ctctgtttgt taaaggcgct
    14521 ctctttgggg atctccggta acacccggac gaacatatcc cagataaagc tcgcaggcag
    14581 cggaaaatca atgtttgccg caataccaaa cttttgcgac agggtcattt gcagccactg
    14641 tgccataccg gtactttgca ccagaatcat ctctggttcg aaaggatcgt ccagccgttc
    14701 gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc agacgattgg aatggtagac
    14761 ccttaacata gcggctcctg actactgacg attcggacaa tgcaggcggg tcatctcgcc
    14821 tgctctgccc ccgggtgaaa ctagcgtaac gctgatgctg acacatcccg cctgcgatgt
    14881 ctgcattcgg ttgacctgcc agttggcagg tggcgaaatc gcgcgcaatt gcgtttgctg
    14941 ccaaccatgc cgccagagct gttggtactg gtttctgctg gtaagactgt tcattaatgt
    15001 tcgctggaaa cccgataacg cagtgacaat catcaccatc aacaccatcg ccaacattac
    15061 ctccggcagg ctaaagcctt gttgattctt cagggaagct gacataacgc cccctctttc
    15121 agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc aatgacttcg
    15181 cctgttcgcc acaacgaaac gccttcataa ccggcaatta ataaggcttc attatcttcc
    15241 agtaaacgca aacaaacccg ggcaccagtg gcagcgtaca gtaagcactg aactgcgggc
    15301 tgcgtctgcc aggagtgcat tttcccccac gccaacgccg actgaacgat ggcctggcgg
    15361 cgcagtgact gactttccat gctcacgcga gaggcaaaac tgcgatcctg ctggctcatt
    15421 ccttgtaata gcaagctacc caaaaccagc agcatcagga ccagagccag tgacgaaaca
    15481 cccttttcgc ggttcacagg ttgaatcctg tcacgctata gctggcatcc accacggttt
    15541 gcggttcaga cttactggca gcacgcatat taaccgtcaa caccggcgag aagccgctga
    15601 catcctgacg tacgacctga aaagtgtcga taatgatggc atccggatta gtgactttat
    15661 cccagccctt accttcacag gatgtcgcac cgcgtagcgt ttccaacact tgctccttca
    15721 gacgaaatcc aatctggtcg gactctttta ccggttcgcg atcccagata ccgttactgt
    15781 tcgcatccca ctgcacaatg atacagtcac cctgtccgac aatttccagc ccttcgccgg
    15841 tacagatgcc atgacaataa cccgccctct ggagatgctt cgcgacggta aatacccgca
    15901 gccagatttc atcttccagt gccagcttac gggtgctcgt taaactttca cgctgtaacg
    15961 caggcagaaa gcgtgcagcc cccaacaaca atacgctact gatcgccata gcaatcaaca
    16021 cttccagcag agaaaaacct tgctctttta caggcatcct tctgtttctc cttgctgaca
    16081 aagccggagt cttccccacg gcgaaaccac cagccaccac tcgcccgttg agtttttgaa
    16141 gccaatatgc ccggcccatg cggtattgcg caggccaaag aaagcaagcg aaggtgtcag
    16201 gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac tgccatgaca
    16261 ggtattcgcc ccagcaacgg aactcacaag gcaccataac gtcccctccc tgataaaact
    16321 gatactgtgg tcgcggttat gccagttggc atcttcacgt aaatagagca aatagtcccg
    16381 cgcctggctg gcggtttgcc aaagccgttg cgactgctgc cagtattgcc agccatagag
    16441 tccacttgcg cttagcatga ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata
    16501 accacgttgt gttttcatgc cggcagtatg gagcgaggag aaaaaaagac gagggccagt
    16561 ttctatttct tcggcgcatc ttccggacta tttacgccgt tgcaggacgt tgcaaaattt
    16621 cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca ctggcgtcgg
    16681 ctctggcagg atgtttcgta attagatagc caccggcgct ttaatgcccg gatgtggatc
    16741 gtagccttca atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt
    16801 gataatcaac ttcggcagcg gacgcggttc acggcttaat tgcagatgag tttgatccat
    16861 atgattactg tacaggtgcg tatcgccacc ggtccagaca aaatcaccca cttccagatc
    16921 gcactgctgc gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc
    16981 gaggaagacg tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata
    17041 gaactggaag aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca
    17101 cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc agctggttca gtaccgtagt
    17161 gatctggtca atatgacgac catctggcgt tggccaggcg cgccactgtt taccatacac
    17221 tggtccgagg tcgccgtttt catcggccca ttcgtcccag atggtgacat tgttttcgtg
    17281 tagataagca atgttagtgt cgccctgcag aaaccacagc agttcatgga tgatggaacg
    17341 caggtggcaa cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg
    17401 atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc
    17461 gagcactttt tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg
    17521 tgggctgcga cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga
    17581 aagaatttgc cccatgctga tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg
    17641 gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca agccagacac
    17701 ggctcccatc gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc
    17761 ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa
    17821 aatggattgc cactgcgggt tggtttgcag cagcaaaata tcttctgtac gggagcctgg
    17881 gaacagcatg gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa
    17941 gttgcccaaa cgcccggcac caagaccaaa cggaatgagt ggtgcgataa aatcagaaac
    18001 ctggaagaag gaacgtttag tacggcgggc aaagataatc atcaccacga taacgccaat
    18061 caggccgccg tggaaagaca tgccgccgtc ccagacacgg aacagataca gcggatcggc
    18121 cataaactgc gggaaattgt agaacagaac ataaccaata cgtcccccga ggaagacgcc
    18181 gaggaagccc gcatagagta agttttcaac ttcatttttg gtccagccgc tgcccggacg
    18241 attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa cccaccagat acatcaggcc
    18301 gtaccagtga agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg
    18361 cagatagcta ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc
    18421 gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg
    18481 acctggctgg ttgccggata ccgttaaaag atatgtatat gatccgcgct ataaccctcc
    18541 gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct gcaacctgat ggcgaacttc
    18601 ggtcgccagt tgcgcttcca gactacgctg cgcaagattt tctgcttcgg cataatcaat
    18661 gcgccgcagc aggtatttta cccgcgctac agaacgtccg ttcatagaca gatggcgata
    18721 cccaagccca atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg
    18781 gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc
    18841 aggatgaaga ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa cggccagaat
    18901 gtattgagtc agatcgttgg tgccaacaga gatgaaatcg acccgctttg ccagatgcgg
    18961 cagcataaat accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc
    19021 gtaaccgatc atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc
    19081 aacttcatcg aggcttgtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt
    19141 agcacgcagc atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg
    19201 aatcccacgc caacccaggc atggattctc ttcgctaatc ggcatgtaag gcagctgctt
    19261 atctgctccg acatccagcg tacgcaaggt gacgggttta tcattgaaca tttgcagcat
    19321 cccctgatac tgcgccacct gttcttcttc cgacggaaaa ccactttgca gcatgaatgg
    19381 gatttcagtg cgataaagac cgatgccatc aatacggctg cccagttttt cttcatgttc
    19441 cgggcttaaa ccagcattga gcatgacttt tatacgctca ccgcttttta actgcgcggg
    19501 taaattgacg tcatcttccg ccagacggct aagctcaatc tcttcactaa ttagccgctg
    19561 atattcttgc agcagtaccg gctccggatc gaccagcaat tcaccgcgat agccatcgac
    19621 gatcagcgtc cgacgatgca gcaccgaagg ctgaatatcc gcgcccatca cggtagggat
    19681 ccccagcgca cgtaccatga tcgcagcatg ggagttggct gcgccatctc gcacgacaac
    19741 accgactaag cgatcctggg gcagctcagc aagagttgtc gctgacagtt catctgccac
    19801 cagaatgaaa cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag
    19861 caatcgctga cccagcgcac gtaaatcgcc agcccgctct ttgagatagt tatcgcttag
    19921 cgcggcaaac tgttcggcaa atttttcaat gaccgttttt accgcccatt ctgccaccga
    19981 gcctttatca acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga
    20041 gtaaagatcg aaaatagccg ccgtttcttt ttgtgcaccg gcggcaaagc gtttgctgta
    20101 gcggcgaaac tcgtttgccg cttcttccag cgccccggtc agtcgttcgc gttccagagc
    20161 cggatccagc gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca
    20221 gccttcggca atcgctacac caggtgctgc cggtaatgcg cggattcgcg tctggcgata
    20281 ttgcccaaac aaggcagtca actgcgactg agaaagaatc gctgccatct gggtggcaag
    20341 cgtcaccagg aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac
    20401 cagtacacca agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg
    20461 ttcttctttt acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt
    20521 tatcggttcc gccagcctgc caaccaggcc gacgatccct tcatcaaagg cgagcgttac
    20581 agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc
    20641 atgatcggcc aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac
    20701 cagaatattt aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg
    20761 caggcgagtg agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt
    20821 ttgtggtttt ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac
    20881 cctacggtag acatcacgtt taaatgacac cacctgtctg accggatacc agtaacttac
    20941 ccatcgccag ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc
    21001 gctcaccagc tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc
    21061 ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat
    21121 tcgaacgtct ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc
    21181 tgcggattct ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg
    21241 ccgggcccac attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta
    21301 gccatcgtca tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt
    21361 catactccag gaagacggta aaccactctc tgcagggcat tacacactaa caacaattga
    21421 ataactcaca gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt
    21481 caccttttct gtggatagag ttgtgaagaa ctacggaatt actacgggaa aacccggagc
    21541 attctgaata agcccgatat agacatttta aaatattcat tattagtcaa tgcattacat
    21601 gtaatttaag taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt
    21661 tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg
    21721 aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag
    21781 gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc
    21841 agtgaccaga tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa
    21901 aagcaatata aatcagataa ttgaacaacg catgtggaaa aagttacact gcgaatattc
    21961 ggcacataat tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca
    22021 ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt
    22081 tatacattgg gagaactggc ggcacttgcc gggctggtta cgccagagaa tttaaaacgc
    22141 gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa
    22201 cctgaacaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt
    22261 ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggagtg
    22321 acctgggaaa ccagccacgt gcgccacaaa ctcaaacgcg tactgtggat accggttgaa
    22381 ggcgagcgca gcatcccgct ggcgcagcgt cgcgttggat caccgttgct gtggagcccg
    22441 aatgaagagg aagaccggca gctacgcgaa gactgggaag aattaatgga tatgattgtt
    22501 ctcggtcagg ttgagcggat taccgctcgt cacggggagt atttacagat acgaccgaaa
    22561 gcagcgaatg cgaaagcgct taccgaagcc attggtgcac ggggcgaacg gattctgacg
    22621 ctgccgcgcg gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt
    22681 ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt
    22741 tttttggcag ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg
    22801 cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt
    22861 ctctggtggt ggcaaagctc cccacagcac aacgtgctca tgcgcgccgt ctggggttgg
    22921 cgggagccat ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga
    22981 cgaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgacttg attctgcttc
    23041 tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg
    23101 aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc
    23161 tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc
    23221 tgtttattat gatggccgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct
    23281 cgattggtga ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc tctttcctga
    23341 ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca
    23401 tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa
    23461 agaacccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac ttctgttacc
    23521 ctcacagttt aagattttcc tgctttcaag atatataacg tcagtttata aacagactat
    23581 tatcataggt gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc
    23641 agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga
    23701 tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag
    23761 ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaataat
    23821 tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt
    23881 cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg
    23941 caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg
    24001 acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct
    24061 cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg
    24121 caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag
    24181 ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg
    24241 gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgtctg
    24301 cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccaac caactgcttc
    24361 ggcaaactcg gttatagctg gacagattct gcgcctgcgg tttcgctgct ggatacgctg
    24421 gacgcactgg cagagtacca acgagcggga aaaattcgtt atatcggcgt gtcgaacgaa
    24481 actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt
    24541 gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa
    24601 gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg
    24661 accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg
    24721 ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc
    24781 agacgtcatg gcctggcccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt
    24841 gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt
    24901 ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat
    24961 acttatccgg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
    25021 gctgccagat ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa
    25081 tgggcacgac cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca
    25141 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
    25201 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc aacacgccaa
    25261 tcagcatcaa caaggcatag gctggcagct gctcgtgttg cagggagaaa atcagtacca
    25321 ccacgccaat caaaattccg gctggcatac agcgtgacac ggtttccagc gtaaccagct
    25381 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg
    25441 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
    25501 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
    25561 tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
    25621 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
    25681 cgtaggccag tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agcacaccac
    25741 cggctacgga accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt
    25801 ttactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
    25861 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
    25921 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
    25981 tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt
    26041 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
    26101 acagtagggc attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
    26161 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccacttcg
    26221 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
    26281 ttcaaatagc gaatatcacg cggtacagca agctccggca cgccgtgctc acgggcatac
    26341 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
    26401 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
    26461 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
    26521 cggccctgaa tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac
    26581 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc
    26641 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
    26701 tcttcgatac cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc
    26761 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca
    26821 ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
    26881 tgtagttttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
    26941 aaacgcgcgt agtgaccgag gaaagtcgaa gtgccgaaca acacggtgca actgcggtca
    27001 tacaccaact ccggcacaat acggtaatgc agcgggcttg gataaagaaa cacttctgca
    27061 cctgtgagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac
    27121 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc
    27181 gccagaatgc ttttatggct atggacgacg ccttttggat ggccttcaga accggaggta
    27241 aaaaggatca gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc
    27301 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
    27361 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
    27421 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct
    27481 tttaccccag cagtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa
    27541 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
    27601 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
    27661 tctggggtaa aattgacgtc ttcgacacat ttcttccctg ctccgaagcg gtacattgca
    27721 ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt
    27781 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
    27841 atctccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
    27901 accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata
    27961 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
    28021 cctgtggtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
    28081 cgtaccagat gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
    28141 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
    28201 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
    28261 attagaacgc gctcgccctt cagtgcctgg gggtcacccg taacgcgaac gcgatacaaa
    28321 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
    28381 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
    28441 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
    28501 cgaataatct caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt
    28561 cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt
    28621 tactgttaca gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa
    28681 ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag
    28741 caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca
    28801 aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg
    28861 aaaacacgcc gccccttgaa ccaacgggcg ttttccgtaa cactgaaaca atgtaagcgt
    28921 ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt
    28981 cagtcgccac cgtttcccgc gtcattaata attcacccaa agccagcgaa gcttcccggc
    29041 tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc
    29101 tggcgcagca gaccactgaa acggtcggtc tggtcgttgg tgatgtttcc gatccgtttt
    29161 tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga
    29221 ttggcaatgg ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc
    29281 atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat
    29341 tattgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc
    29401 gttgtattgc tctggacgat cgttacggtg cctggctggc tacgcgtcat ttaattcagc
    29461 aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc
    29521 gtctgcaagg gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg
    29581 tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac
    29641 gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg
    29701 gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg
    29761 atgtgctggt gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga
    29821 cgatggcgac ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg
    29881 aaatcactaa tgtctttagt ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc
    29941 tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt
    30001 aattcttcga tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact
    30061 tctggtaaca gcggacggct attgtagttg gatgacattg atgcgccata tgcccctgta
    30121 tcatgcagta ccagataatc acctgccttc acttccggca aggcgcgggg ttcaacattt
    30181 cccccttcct gctgggtaaa gacatcgccc gattcacata acggtccggc gacgacggtt
    30241 tccaccgttg gtgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg
    30301 taactaccgt acattgccgg gcgcatcaga tcgttaaacc cggcatcaac cagcacaaag
    30361 tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaacacgcc agactgcgct
    30421 accaggaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt
    30481 tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc
    30541 tgttgataag gaatagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat
    30601 tcgatgacct gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca
    30661 gaaccaatgt gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt
    30721 gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt
    30781 ttttggctat gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc
    30841 gaaacctggc ccagttggtc gaacatatca acagaacccg cattcaccgg aatttgcaat
    30901 tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg
    30961 gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg
    31021 gaatccactt tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct
    31081 ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt
    31141 tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg
    31201 gcggtgagat cggtatcggt gctgaacagt gaatgtggca taacaaactc cagatgagtg
    31261 cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct
    31321 atgcaaaaat gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta
    31381 cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac
    31441 ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc
    31501 gggctgaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt
    31561 ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa
    31621 agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa
    31681 tcttttttac cgcagctcct gcaacccttt ctggtacgtt atcccgatgt cagcttaaat
    31741 atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta
    31801 ggactcactg agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta
    31861 gatgaattgt gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg
    31921 gatgattttc agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg
    31981 ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc
    32041 gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc
    32101 accgcactgg attatgcggc aagcggttta gtggtgcggc ggttcagtat tgcggttccg
    32161 ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt
    32221 agtgagcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg
    32281 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca
    32341 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
    32401 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
    32461 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
    32521 catccgcttc aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa
    32581 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
    32641 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
    32701 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
    32761 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
    32821 gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
    32881 gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt
    32941 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac
    33001 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta
    33061 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt
    33121 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt
    33181 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
    33241 gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat
    33301 attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg
    33361 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
    33421 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
    33481 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
    33541 tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa
    33601 cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc
    33661 cgccattttg aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg
    33721 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
    33781 cagtgcccaa ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
    33841 cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc
    33901 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
    33961 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt
    34021 atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat
    34081 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc
    34141 gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc
    34201 aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt
    34261 acgccccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
    34321 catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa
    34381 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
    34441 agctaccgaa acaaacatat tcatacgccg cgtatcacgc aaagaacgtg gcgttaaagc
    34501 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
    34561 aatacgtgaa caattcacac aggtgtcagg tcggaaacag cataaatatg gattaaattg
    34621 ctgcgacatg ccgttatgtg atggatattc caattttaaa
//
LOCUS       DAZFKY010000029        34204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFKY010000029 DAZFKY010000000
VERSION     DAZFKY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(80..755)
                     /locus_tag="ACHKCL_001270"
                     /pseudo
     CDS             complement(80..755)
                     /locus_tag="ACHKCL_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(900..1508)
                     /gene="yiiX"
                     /locus_tag="ACHKCL_001271"
     CDS             complement(900..1508)
                     /gene="yiiX"
                     /locus_tag="ACHKCL_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5762276.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1568..1885)
                     /gene="metJ"
                     /locus_tag="ACHKCL_001272"
     CDS             complement(1568..1885)
                     /gene="metJ"
                     /locus_tag="ACHKCL_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5762277.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2162..3322
                     /gene="metB"
                     /locus_tag="ACHKCL_001273"
     CDS             2162..3322
                     /gene="metB"
                     /locus_tag="ACHKCL_001273"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5762278.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3325..5757
                     /gene="metL"
                     /locus_tag="ACHKCL_001274"
     CDS             3325..5757
                     /gene="metL"
                     /locus_tag="ACHKCL_001274"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5762279.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5896..6030)
                     /locus_tag="ACHKCL_001275"
     CDS             complement(5896..6030)
                     /locus_tag="ACHKCL_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762280.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6106..6996
                     /gene="metF"
                     /locus_tag="ACHKCL_001276"
     CDS             6106..6996
                     /gene="metF"
                     /locus_tag="ACHKCL_001276"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5762281.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7325..9505
                     /gene="katG"
                     /locus_tag="ACHKCL_001277"
     CDS             7325..9505
                     /gene="katG"
                     /locus_tag="ACHKCL_001277"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5762282.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9599..10504
                     /gene="yijE"
                     /locus_tag="ACHKCL_001278"
     CDS             9599..10504
                     /gene="yijE"
                     /locus_tag="ACHKCL_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5762283.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10531..11148)
                     /gene="yijF"
                     /locus_tag="ACHKCL_001279"
     CDS             complement(10531..11148)
                     /gene="yijF"
                     /locus_tag="ACHKCL_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5762284.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11422..12525)
                     /gene="gldA"
                     /locus_tag="ACHKCL_001280"
     CDS             complement(11422..12525)
                     /gene="gldA"
                     /locus_tag="ACHKCL_001280"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5762285.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12536..13198)
                     /gene="fsa"
                     /locus_tag="ACHKCL_001281"
     CDS             complement(12536..13198)
                     /gene="fsa"
                     /locus_tag="ACHKCL_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5762286.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13210..15711)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_001282"
     CDS             complement(13210..15711)
                     /gene="ptsP"
                     /locus_tag="ACHKCL_001282"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5762287.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16020..17099
                     /gene="frwC"
                     /locus_tag="ACHKCL_001283"
                     /pseudo
     CDS             16020..17099
                     /gene="frwC"
                     /locus_tag="ACHKCL_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
     gene            17114..17434
                     /gene="frwB"
                     /locus_tag="ACHKCL_001284"
     CDS             17114..17434
                     /gene="frwB"
                     /locus_tag="ACHKCL_001284"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5762288.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17485..19782
                     /gene="pflD"
                     /locus_tag="ACHKCL_001285"
     CDS             17485..19782
                     /gene="pflD"
                     /locus_tag="ACHKCL_001285"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5762289.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19748..20626
                     /gene="pflC"
                     /locus_tag="ACHKCL_001286"
     CDS             19748..20626
                     /gene="pflC"
                     /locus_tag="ACHKCL_001286"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5762290.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20628..20969
                     /gene="frwD"
                     /locus_tag="ACHKCL_001287"
     CDS             20628..20969
                     /gene="frwD"
                     /locus_tag="ACHKCL_001287"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5762291.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20956..21807)
                     /gene="yijO"
                     /locus_tag="ACHKCL_001288"
     CDS             complement(20956..21807)
                     /gene="yijO"
                     /locus_tag="ACHKCL_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5762292.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22022..23755)
                     /gene="eptC"
                     /locus_tag="ACHKCL_001289"
     CDS             complement(22022..23755)
                     /gene="eptC"
                     /locus_tag="ACHKCL_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5762293.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23938..26589)
                     /gene="ppc"
                     /locus_tag="ACHKCL_001290"
     CDS             complement(23938..26589)
                     /gene="ppc"
                     /locus_tag="ACHKCL_001290"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5762294.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26941..28092)
                     /gene="argE"
                     /locus_tag="ACHKCL_001291"
     CDS             complement(26941..28092)
                     /gene="argE"
                     /locus_tag="ACHKCL_001291"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5762295.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28246..29250
                     /gene="argC"
                     /locus_tag="ACHKCL_001292"
     CDS             28246..29250
                     /gene="argC"
                     /locus_tag="ACHKCL_001292"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5762296.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29258..30034
                     /gene="argB"
                     /locus_tag="ACHKCL_001293"
     CDS             29258..30034
                     /gene="argB"
                     /locus_tag="ACHKCL_001293"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5762297.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30095..31468
                     /gene="argH"
                     /locus_tag="ACHKCL_001294"
     CDS             30095..31468
                     /gene="argH"
                     /locus_tag="ACHKCL_001294"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5762298.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVARAIA
                     FARARLG"
     gene            31735..32652
                     /gene="oxyR"
                     /locus_tag="ACHKCL_001295"
     CDS             31735..32652
                     /gene="oxyR"
                     /locus_tag="ACHKCL_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5762299.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32635..34035)
                     /gene="sthA"
                     /locus_tag="ACHKCL_001296"
     CDS             complement(32635..34035)
                     /gene="sthA"
                     /locus_tag="ACHKCL_001296"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5762300.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQLLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7724 a         8644 c         9550 g         8286 t
ORIGIN      
        1 tggctgtgta ttcagtctgg taatcatcca aggaaagctc atcagaaggg tgatgactcc
       61 aattagtaga acatatattt tcgataaaag caccgatgac tttttcatga agttctacag
      121 aactcgagaa acagatggtt ctgcgggcca gacgtttgat tcgggttctc agggtcagat
      181 tgttacgctc aatgtgctga gtaaaaatct taccagtcag atgcttctcc tttggtaact
      241 ctctagcgta actaccccat tcgtcagtgg tcaacatgcc gatggtaaaa gggtaagcag
      301 cgccagcaat tcacgacagg tttcatcagt ccgggggcca aaagtgtagg ccagaacacc
      361 accagttttc gtgttgtaca cgtaccagag ccagtgctgt cgcgctttac tgccgacaaa
      421 gctccattgc tgactaagtt tgcagatgag tgccacatca gcatgagcga ccggggatga
      481 cgttattctt cgtggcgaga gttttttaaa gttcggatga ctgtattaat gccgattttc
      541 agcgtacggg cagtatagcg aaccccggca ccgttgaaag ccatttcagc gatttggtct
      601 ttaactcccg gtttacgggg ctcgtaacag taactgagct gaaatacccg gtgacagtca
      661 cggccgcgga atcgatcatg tcttttgggg ttctgcccgc gacggtaaac ctgaacggac
      721 tggcaacggg gacaatgaag gttaactgtg accatgaaac atccttaaaa gcgagtatta
      781 tatccttaat tcaactaatt ggaggcatca ccgtttaccg gagcgacagc ttgcggaaag
      841 gtcgtgcaac tacaaagcaa caaacagact acggcgggaa ttattttttt catcatccat
      901 taccacgaaa acagcggcca ttctttggca accgtggtga gttgtggcgc atcgaaaacg
      961 gcttgtggtg agaccaccgt ttcctcaagc ggaatatttt tgccgtagcg ttctttgagc
     1021 ttcgcctgga ccagcgggtt gctgagatca aactctttca acttctgctg ttcgcccaca
     1081 cgcattccca gcgcattctg ataaaccttc cacaccactt ccgaacagta ctggcgatcg
     1141 tcgctccatg agaagctgaa atcgtacggt ttgccgagat aacgtttcgc cgtttgcgcc
     1201 agtttttgct gttgttcaac actcagtccg ccttcaacgc ggcgaacaac gtatttgccc
     1261 ttttcaccat gggcaatcca ctgcttgagc ggggtgtatt tcaccgggcc gaccgcttca
     1321 aaaacgtagg gctttttgtt acgcatcacc agcataccgg tgtggctgta atcggaatgg
     1381 gtcgccagtt ggatcgcttt actttgcgat gagcgagaga tttgaaagat gatgtcgcca
     1441 gtttgcggct gccaggcaaa ggcaggcaca gaaaccagca ggctgaggat cagtagcctg
     1501 attttcatcg ttaaacgtcc ttgtatgaaa aaagcggctg gttaactccg cgccgctctt
     1561 ttccgcttta gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg
     1621 ccgcttccgg gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt
     1681 gcccggtaaa ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca
     1741 ggttgttcac ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa
     1801 tggaaaccgt aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga
     1861 tatattcgcc gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac
     1921 cagaccacac aatttatgtg ggtactgacc gtaaacccgc atagtttacc gtacaggcgt
     1981 taccgtgaca tcgtgtaatg cacctgtcgg cgtgataatg catataattt taacggctat
     2041 ttgggatttg ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat
     2101 taacacagtg tttactctgg tgcctgacat ttcaccgacc aagcccaggg aacttcatca
     2161 catgacgcgt aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta
     2221 tggttgcgtt gtccctccca tccatctttc cagcacctat aactttaccg gatttaatga
     2281 accgcgcgcg catgattact cgcgtcgcgg caacccaacg cgcgatgtgg ttcagcgtgc
     2341 gctggcagaa ctggaaggcg gtgctggtgc agtacttact aataccggca tgtccgcgat
     2401 tcatctggta acgaccgtct ttttgaaacc tggcgatctg ctggttgcgc cgcacgactg
     2461 ctacggcggt agctatcgcc tgttcgacag cctggcgaaa cgcggttgct atcgcgtgtt
     2521 gtttgttgat caaggcgatg aacaggcatt acgggcagcg ctggcagaaa accccaaact
     2581 ggtactggta gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg
     2641 ccatctggca agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc
     2701 attacaaaat ccgctggcat taggtgccga tctggtgttg cattcatgta cgaaatatct
     2761 gaacggtcac tctgacgtgg tggctggcgt ggtgattgct aaagacccgg acgttgtcac
     2821 tgaacttgcc tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct
     2881 gctgctacgc gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc gcaacgcgaa
     2941 ggcgattgtg aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct
     3001 gccggaaaat caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt
     3061 gagttttgaa ctggatggcg atgagcagac gctgcgccgt ttcctgggcg ggctgtcgtt
     3121 atttacgctg gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat
     3181 gacacatgca ggcatggcac cagaagcgcg tgctgccgcc gggatctccg agacgctgct
     3241 gcgtatctcc accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt
     3301 ccgggctgca aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt
     3361 cagctgcata aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg
     3421 ggcattatgg cggagtactc tcagcctgac gatatgatgg tggtttccgc cgctggtagc
     3481 accactaacc agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat
     3541 caggttcaac aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgcc
     3601 gaagaagccg atagcctcat tagcgctttt gtcagcgatc ttgagcgcct ggcggcgctg
     3661 ctcgacagcg gtattaacga cgcagtgtat gcggaagtgg tgggccacgg cgaagtgtgg
     3721 tcggcgcgcc tgatgtctgc ggtgcttaat caacaagggc tgccagcggc ctggcttgat
     3781 gcccgcgagt ttttacgcgc tgaacgcgct gcgcaaccgc aggttgatga agggctttct
     3841 tacccgttgc tgcaacagct actggtacaa catccgggta aacgtctggt ggtgaccggg
     3901 tttatcagcc gtaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat
     3961 tccgcgacac aaatcggtgc gctggcgggt gtttctcgtg taaccatctg gagcgacgtc
     4021 gccggggtat acagtgccga tccgcgtaaa gtgaaagatg cctgtctgct gccattgctg
     4081 cgtctggatg aagccagcga actggcgcgt ctggcggctc ccgttcttca cgcccgtact
     4141 ttacagccag tatccggtag cgaaatcgac ctgcagctgc gctgtagcta cacgccggat
     4201 caaggctcca cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc
     4261 agccacgatg atgtctgctt gattgagttt caggtgccca ccagccagga tttcaaactg
     4321 gcgcataaag agatcgacca gatcctgaaa cgtgctcagg tgcgcccact ggcggttggc
     4381 gtgcataacg atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg
     4441 ctgaaaatcc tcgacgaagc gggattacct ggcgaactgc gcctgcgtca ggggctggcg
     4501 ctggtggcga tggtcggggc gggtgtcacc cgtaacccgc tgcattgcca ccgcttctgg
     4561 cagcaactga aaggccagcc ggtcgagttt acctggcagt ccgatgacgg catcagcctg
     4621 gtggcagtac tgcgcaccgg cccgaccgaa agcctgattc agggcctgca tcagtccgtc
     4681 ttccgcgcag aaaaacgcat cggcctggta ttgttcggca aaggcaatat cggttcccgc
     4741 tggctggaac tgttcgcccg cgagcagagc acgctttcgg cgcgtaccgg ctttgaattt
     4801 gtgctggcag gcgtggtgga cagccgccgc agtctgttga gttatgacgg gctggacgcc
     4861 agccgcgcgt tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc
     4921 ctgtggatgc gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag
     4981 cagctggctg accagtatct tgatttcgcc agccacggtt tccacgtcat cagtgccaac
     5041 aaactggcgg gagcaagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa
     5101 accgggcgtc actggctata caacgccacc gtcggtgcgg gcttgccgat caaccatacc
     5161 gtgcgcgatc tgatcgacag cggcgatact attttgtcga tcagtgggat cttctccggc
     5221 acgctctcct ggctgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat
     5281 caggcgtggc agcagggctt aaccgagcct gatccgcgtg acgacctctc cggcaaagac
     5341 gtaatgcgca agctggtgat tctggcgcgt gaagcgggtt acaacattga accggaccag
     5401 gtacgtgtgg aatcgctggt gcctgctcat tgcgaaggcg gcagcatcga ccatttcttt
     5461 gaaaatggcg atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg
     5521 gggctggtgc tgcgctacgt ggcgcgtttc gatgccaacg gcaaagcgcg tgtgggcgtg
     5581 gaggcggtgc gtgaagacca tccgctggca tcactgctac cgtgcgataa cgtctttgcc
     5641 atcgaaagcc gctggtatcg cgacaacccg ctggtgatcc gtgggcctgg cgcggggcgc
     5701 gacgtcaccg ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt
     5761 cagaaattta ataatgcccg gtactcatgt tttcgggttt atggtttcta atgaaatata
     5821 ttgaattatc ataggattag gccggatgaa gcgtttacga cgaatccggc aagaagcaat
     5881 aagtacatgg ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt
     5941 cttaccatcc tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt
     6001 tcggttgacg cccttcggct tttccttcat ctttacatct ggacgtctaa acggatggat
     6061 gtgcacaaca caacatataa ctacaagcga ttgatgaggt aaggtatgaa cttttttcac
     6121 gccagccagc gggatgccct gaatcagagc ctggcagaag tccaggggca gattaacgtt
     6181 tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc
     6241 gatcgcctta gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc
     6301 gagcgcgacc gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg
     6361 gcaccgcatc ttacttgcat tgatgcgacg cccgacgagt tgcgcaccat tgcacgcgac
     6421 tactggaata acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt
     6481 ggtaagccag aaatgtatgc ttctgacctg gtgacgctat taaaagaagt ggcagatttc
     6541 gatatctccg tggcggcgta tccggaagtt cacccggaag caaaaagcgc ccaggcggat
     6601 ttgcttaacc tgaaacgcaa agtcgatgca ggagccaacc gcgcgattac tcagttcttc
     6661 tttgatgtcg aaagctacct gcgttttcgt gaccgctgtg tgtcggcggg cattgatgtg
     6721 gaaatcattc cgggcatatt gccggtatct aactttaaac aggcgaagaa atttgccgac
     6781 atgaccaacg tgcgtattcc ggcgtggatg gcgcaaatgt ttgacgggct ggatgacgat
     6841 gccgaaaccc gcaagctggt gggcgcgaat attgccatgg atatggtgaa gattttaagc
     6901 cgtgaaggag tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg
     6961 atttgccata cgctgggggt tcgacctggt ttataaatag tgtggctttt atgaaaatca
     7021 cacagtgatc acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgggaaa
     7081 ataaggttat cagccttgtt ttctccctca ttacttgaag gatatgaagc taaaaccctt
     7141 ttttataaag catttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt
     7201 gatctacatc tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat
     7261 tcaattataa cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac
     7321 attgatgagc acgtcagacg atatccataa cacgacagcc actggcaaat gcccgttcca
     7381 tcagggcggt cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa
     7441 tcaactccgt gttgacctgt taaaccaaca ttctaatcgt tctaacccgc tgggtgagga
     7501 ctttgactac cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa
     7561 agccctgctg acagaatctc agccgtggtg gccagccgac tggggcagct acgccggtct
     7621 gtttattcgt atggcctggc acggcgcggg gacttaccga tcaatcgatg gacgcggtgg
     7681 cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc tggccggata acgtaagcct
     7741 cgataaagcg cgtcgcttgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg
     7801 ggccgacctg tttatcctcg cgggtaacgt ggcgctggaa aactccggct tccgtacctt
     7861 cggttttggt gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga
     7921 tgaaaaagcc tggctgactc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc
     7981 aaccgagatg ggtctgattt acgttaaccc ggaaggcccg gatcacagcg gcgaaccgct
     8041 ttctgcggca gcagctatcc gcgcgacctt cggcaacatg ggtatgaacg acgaagaaac
     8101 cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc cacggtgctg gtccgacatc
     8161 aaatgtaggt cctgatccag aagctgcgcc gattgaagaa caaggtttag gttgggcgag
     8221 cacttacggc agcggcgttg gcgcagatgc cattacctct ggtctggaag tggtctggac
     8281 ccagacgccg acccagtgga gcaactattt cttcgagaac ctgttcaagt atgagtgggt
     8341 acagacccgc agtccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat
     8401 cccggatccg ttcgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac
     8461 gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc
     8521 gttcaacgaa gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa
     8581 atctcgttac atcgggccgg aagtaccgaa agaagatctg atctggcaag atccgctgcc
     8641 gcagccgatc tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga
     8701 ttctggtctg tctgttagtg agctggtatc ggtggcctgg gcttctgctt ctaccttccg
     8761 tggtggcgac aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga
     8821 ctgggatgtg aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa ttcagaaaga
     8881 gtctggtaaa gcctcgctgg cggatatcat cgtgctggca ggtgtggttg gcgttgagaa
     8941 agccgcaagc gccgctggtt tgagcattca tgtaccgttt gcgccgggtc gcgttgatgc
     9001 gcgtcaggat cagactgaca ttgagatgtt cgaactgctg gagccaattg ctgacggttt
     9061 ccgtaactat cgcgctcgtc tggatgtttc caccaccgag tcactgctga ttgataaagc
     9121 ccagcaactg accctgaccg cgccggaaat gaccgcgctg gtgggcggga tgcgtgtact
     9181 gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag
     9241 caatgacttc ttcgtgaact tgctggatat gcgttacgag tggaaagcga ccgacgaatc
     9301 gaaagagctg ttcgaaggcc gtgaccgcga aaccggcgaa gtgaaataca ctgccagccg
     9361 tgcggatctg gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag tttacgccag
     9421 cagcgatgct cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa
     9481 cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag cgactgcctt tcaggcagtc
     9541 gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat
     9601 gtctgccgca ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat
     9661 ctggagttat agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt
     9721 taccgcctta cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg
     9781 tcgcggaatg cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg
     9841 cgggatggtt ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat
     9901 cctgagctat accatgccgt tctgggtggt gattttcgcc gcggtgtttc tcggtgaacg
     9961 cctgcgacgt gggcaatatt tcgcgattct gattgccgct ttcggcttat ttttggtgtt
    10021 gcagccgtgg caactcgatt tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg
    10081 tgttagttgg ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt
    10141 ggatttattg tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt
    10201 cgctttactg gtgccgcaac gtgaaattga ctggcagccc actgtgttct gggctctggc
    10261 ctacagtgcg attctggcga cggcgttggc gtggagctta tggctgtttg tgctgaaaaa
    10321 cttgcctgcc agtattgcca gcttaagcac actggccgtt cccgtttgcg gcgtactctt
    10381 ttcctggtgg ctgctcggtg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat
    10441 tgtgctggca ctggcgctgg tgagccgtaa gaaaaaagaa gccgttagtg taaaaaggat
    10501 ctgaattttt tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac
    10561 catgcgccag ctaaacagca catcttcttc ctgcgcacct gcgccaatgt tatgtatcac
    10621 cagcggcgta ccgtcgcggg cgaagccatc tgaaaccacc ccaatatgtg ccagcccgtt
    10681 atctaatcgc caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg
    10741 gcgtgtttta tcgtggcggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat
    10801 gttgctgtcc gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat
    10861 atcttcgtgc accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac
    10921 atcggaacat accccgcgtt cttgcgggac atcaccgcca ggataggtaa gctgcacata
    10981 cgccggatcg taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag
    11041 gttggtattg gcctggattt gtaccacggt tggtggaatg gcgggagatt ttaaggagta
    11101 gctggtaaat gccgttagca ggctgaccag cgccagtgaa gctttcatct gtttcgtccg
    11161 tgaaaataag agtcacagat tcgaccttcc gggctaacga ttgttctgta gagaaaatgt
    11221 ccagaagcgg ataaaaaatt cctgaacaac gtgggagttt cagattactg gcacagagtg
    11281 ccggatgcgg cgtgaacgtc ttatctggcc taaaaagcac gcaaattcaa tatattgcag
    11341 agattgcgta ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac
    11401 aagccgggag tttggagtag gttattccca ctcttgcagg aaacgctgac cgtactggtc
    11461 ggctaccagc agagcggcgt aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat
    11521 ggtttcacct tctgcacatg ccgcttctgc cacaattcgc attttcgccg ggacatcttc
    11581 tttaatatcc agttgagcga gagttattgg caaacctacc gcatggctaa gcgcagctac
    11641 ggtttcgatt tcctcaaccg gcgagttttc cagtaccagc tgcgtcagtg taccaaatgc
    11701 cactttttca ccgtgataat agtgatgggc atcaggaatg gcagtcaggc cattatgcac
    11761 cgcgtgcgct gccgccagac caccgctttc aaagccaaca ccgcttaaat aagtgttcgc
    11821 ttcaatcacg cgttccagtg ccggagtcac tacatgctgt tcggcggcga gcatcgcttt
    11881 ttcgccttct tccagcaagg tgttgtagca cagttcagcc agcgccagcg ccgcctgagt
    11941 gcacttgccg cccgccatgg tggtcgcacc gctacgagag caggcacgcg cttcaaacca
    12001 ggtcgccagc gcatcgccga tacccgccgc taacagacgc gcaggtgcgc ccgcgacgat
    12061 tttagtatcg acaatcacca tattcgggtt atttggcagc agcagatagc ggtcaaattc
    12121 accttcgtca gtgtagataa cggaaagagc gctacaaggt gcgtcggttg aagcaatggt
    12181 cggggcaatg gcgaccggaa cacccatgaa atgtgccagt gctttggctg tatcgagggt
    12241 ttttccgcca ccgataccga gaattgcgcc acactgcgca gtctccgcga tgccacgcag
    12301 acggtcgatc tcattttgcg aacattcacc gccaaacggc gcaatctcta ctaccagtcc
    12361 agcatcttta aagcttttct cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc
    12421 caccactaac cagcgttctg ccagcggctt caggtattcg cccagacgat taatcacatc
    12481 agcgccctgg atgtatttac ccggtgattg aataatgcgg tccataattg ctcctttaga
    12541 gatgagtagt gccaaatgcg gcattccagt cgtgttcgaa cttctctata gctgactcta
    12601 ccgcaggggt gttgagcatt tgttgcgcta catctaaggg cagggtgatg gattcacatc
    12661 ctgccagtaa acagtccagc gcctgacgcg gcgttttaaa gctggccgcc agcaccatgc
    12721 tttctggtgc gtgcatttct aacagcgctt gcagctcctg aaccgtacga atgccgtccc
    12781 cgccctgggc atccacgcgg ttaacatacg gagcaacgta ttttgcccct gccagtgcgg
    12841 ctaataaccc ttgtgcggcg ctatatacag cagtgccaag tgtagtaata ccctcttttt
    12901 tcagtatttt aattgctgcc agaccttcgg aagtcaccgg gattttcacc acaatgcccg
    12961 gaatggcatc gcgcagacgc ttcgcttctt ccaccatccc ctgcgcgtcg cggctcatgg
    13021 tctgagcaaa cagaatgccc tcatcaccaa tcgctttttg cagacgcggc agcacttccc
    13081 atatggactc cttgctggca gcgataatgc tcgggttagt tgtcaccccg gcaatcggga
    13141 atatgtgtgc cagacgttcg acttctgcga cgttagcggt gtccagatac agttccatga
    13201 tgtttccttt tacagttcca gttcatgttg cagcaggctg gcgatagcgt cggcagaggc
    13261 ggcgttaacc agcgcgttac ggaattcttc gtgcatgatg cggcgagcga ggcgcgagaa
    13321 aatgcgcata tgctgatcgc ccgcagcgtg tttgttcagg gttaacataa tgatgaattg
    13381 cgcttcatca tcgccccagc gcaccggcgc ttgcagacgc gctacgctga tggtggattg
    13441 ctcaatgtgt tctgatttgc tgtggggaat ggcaaaacta aagcccagac cggtagagaa
    13501 aacggcctcg cgcgcccaca agtcggcttc cagtttacgc ggatagcggc agcggcccgc
    13561 cagcagcagg ttatcggtca tgcctttgag cacttcttct ttgctgcgcc agtcgctttc
    13621 cagtgtgatg cactcggcgg tgaccagcgg tgcgtcttgt tgggtcatgc ggaattgcgc
    13681 cagcaggtgt tccacttcca gcgaagtacg gcaggccatt gcctggttga gcaacttgcg
    13741 gcactcacgg ctatcaagtt gcgccatccg cgctttcgcc gccggaattg atggtgcgct
    13801 catgctgagt tcatccagcc ctaagccgac cagcaacggc agcacggaac ctttcgctcc
    13861 cagctcaccg cacagaccaa tccatttgcc ctggcgatgc accgcttgca cggcgtaatc
    13921 gagcgcccgc aagaatgccg gattcaggct gttgtagtga cgagtaacct tagcgttatc
    13981 gcgatccact gccagcagat attgcgtcag gtcattacta ccaatactaa agaaatcaat
    14041 ctcttcgcag cattgatcga tgatgaacat caccgacggc acttccagca tgatgccgag
    14101 ctggatcttc tcatcaaacg gaatgtgttc gttacgcagt tgctgtttgg cttccgccag
    14161 tttttctttc acccataaga tctcttccat tgaggagatc atcgggatca tgattttcag
    14221 gctgccgtga gcagaggcac ggaggatcga ccgtagttgt gtggtgaaca gcgaggcgta
    14281 ttcttcataa atacgcaccg cgcgataacc gaggaacggg ttagcttctg ccggaatgtt
    14341 cagataatca acgggtttgt caccgccaat atccatagtg cgcacaataa tgctgcgtcc
    14401 gttggcggac tccagcgctt gacaaaaaat gttgtacaac tcgctttcgc ccggtgcgct
    14461 ggtgcgatcc atatagagca tttcagtgcg gaacaaaccg acgccttccg caccattgcc
    14521 gaatgcggcc tgcgcttcca cggagtgagc gatgttagcg gcaatttcaa tgcggatacc
    14581 gtcagcggta cgggcttgtt gggtcagcca gacacgctgt tgctcacgca gggcgtcctg
    14641 tacgcgggct tcttgctgat aataacgagc tacggcttcc cctggctcaa ccacaatcgc
    14701 cccggcgttg ccgtcgatat aaatcgtttg atgctgccac ggagtaaggg catcaatatc
    14761 tacaccaacc agtgtcggaa tgttgaacga acgggcaaga attacggtat gtgaggtggt
    14821 gccgccactt ttcagtaaca gacctttgag gtgattttta tccagttcga ggaactggct
    14881 gggggttagt tcatcagcca tacaaatggc gggctgcgtc agtttgcccg gtgccgggaa
    14941 gcgttgctca ccgtagattt gctgaagcag ctggaagcag acatcgcgta cgtccagggc
    15001 acgttcttgc aggtagctgc tgctggaacg ggaaaactct tcacaaaagt gattcgcact
    15061 ggcgacaatc gcttcggcgc agcttaatcc ggcgctgacg cctgccagta aatgttcgtg
    15121 cagggaagta tcgccagcca gggatcggtg cgcttccaga atcgcgctgg tggcaccgtc
    15181 gctatccagc agacgaaact caatgttttt cagtaccagc gtcaggccgt tttccagtgc
    15241 ggattgctcg gcgtcaacgt ctttggctgc gggaagatta cccagcgcat tgagatctaa
    15301 agaagagatc ggcgtcagaa tgccgcctgc gctaccgctg cacacggtgc gggcgcggat
    15361 aatttgcgga ttcagattgg tcagtgaaac cggcagtggt tccagttcgt cagatttaac
    15421 ttccgccagc ggcgcatcgc agtgggggaa ttcatcgcgc agccattggc ttaaacgctg
    15481 gtgcgcttcc tgttcgtcgg ccccggaaat cagtaactgg cagttatcgc ccgccagcgt
    15541 gtcggtgcca atcagcgcaa gggcgctttt ggcgttgccc ttgcggtcag tgcgcaggtt
    15601 atgccactca atttgtgatg aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg
    15661 tgcatgtacg ccgttaggta gctcacaaat aaattccaca atcagggcca ttgcctctct
    15721 cccggtacgg ttttctgttt acaggataag agcgcacggc aacggcctgc catgtgacaa
    15781 atctgccaaa agctggacaa atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac
    15841 gccgttttgc ggcatgcttc cggattatcg caagttatga ggcggatcgc atttttgtac
    15901 tgatattaca aaagtccagt aaatggcctt tttatccact gtttgctccg cctgcgattg
    15961 cctattgttt cctccatcaa aaatatggac atgggctttc gcaagcccgg gagcagctta
    16021 tgaatgagtt ggtgcagatc ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac
    16081 acatgattcc cttcgtggta tcgggcggta ttttgctggc ggtttccgtc atgttgtatg
    16141 gcaaaggcgc agtgccggat gccgtagccg atccgaatct gaaaaaactg tttgatatcg
    16201 gcgttgcagg tttgacgctg atggtgcctt tcctcgccgc ttacatcggt tactccattg
    16261 cagagcgttc tgcgctggct ccgtgcgcta tcggtgcctg ggttggtaac agctttggtg
    16321 cgggcttctt tggtgcactg atcgccggga ttatcggcgg catcgtggtg cattacctga
    16381 agaaaattcc ggtgcataaa gttctgcgct cggtgatgcc catcttcatc attccgatcg
    16441 tcggcacact gattaccgca ggcatcatga tgtggggtct gggcgagcct gtaggggcgt
    16501 tgaccaacag cctgactcag tggcttcagg ggatgcagca gggcagcatt gttatgctgg
    16561 cggtgatcat gggtctgatg ctggcgttcg atatgggcgg tccggttaac aaagtggcct
    16621 atgccttcat gctgatttgc gttgctcagg gtgtttatac cgtggtggcc attgctgccg
    16681 tgggtatttg tatcccgccg ctggggatgg ggctggcgac gctgattggt cgtaaaaatt
    16741 tctccgcaga agagcgctaa accggtaaag cggcgctggt gatggggtgc gttggggtta
    16801 ctgaaggggc gattcctttc gccgcagccg atccgttacg tgtaattcct tccatcatgg
    16861 ttggttcagt ttgtggtgca gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct
    16921 ggggtggtct gattgtgctg ccggtggttg aaggtaagtt gggttatatc gcagcagtgg
    16981 ctgtcggggc ggttgtgacg gcagtctgcg tgaacgtgct gaaaagtctg gcgcgtaaaa
    17041 atggatcttc tactgatgaa aaagaagacg acctggattt ggattttgaa attaattaat
    17101 tgaggaacga gccatgacga aaattattgc agtaaccgcg tgtccatctg gtgttgccca
    17161 tacttatatg gctgcagagg cgctggaaag tgccgcgaaa gcgaaaggct gggaagtgaa
    17221 agtagaaacc cagggctcga ttggtctgga aaacgaactg actgcggaag acgtggcgag
    17281 cgccgatatg gtgattctga ctaaagatat cggcatcaag tttgaagagc gttttgctgg
    17341 caagaccatc gtgcgcgtca acatcagcga tgcggtaaaa cgtgctgatg ccattatgag
    17401 caaaattgaa gctcacctgg cgcaaaccgc ttaagttctt ctcccgctcg caagggcggg
    17461 ttcgctttcc cacaggagtt cctcatgacg aatcgtatct ctcgcctcaa aactgcactg
    17521 tttgccaata cccgtgaaat ctcgctggag cgtgcgctgc tttataccgc cagccatcgg
    17581 caaaccgaag gcgaaccggt gatattgcgc cgggcgaaag caacagcgta tatccttgaa
    17641 catgttgaaa tttcgattcg tgatgaagaa ctgattgccg gtaaccgcac cgtaaaaccg
    17701 cgcgccggga ttatgtcgcc ggaaatggac ccttactggc tgctgaaaga gctgggtcaa
    17761 ttcccgacgc gtccgcagga ccgttttgct atcagcgaag aagataaacg tatctaccgc
    17821 gaagagttgt tcccgtactg ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg
    17881 acggatgaag taaaagccgc gactagcacg cagattttca gcatcaacca gacggataaa
    17941 ggccaggggc acattattat tgattacccg cgcctgctga atcacgggct gggggagctg
    18001 gtggcacaga tgcagcaaca ttgtcagcaa cagccggaga atcactttta tcaggcagcg
    18061 ctgttactgc tcgaagcctc gcagaaacac attttgcgtt acgccgaact ggcggaaacg
    18121 atggcggcaa gctgcactga tgcccagcgt cgcgaagaac tgttgactat tgcggagatc
    18181 tcccgccata acgccgaaca taaaccgcag acgttctggc aggcgtgcca gttattctgg
    18241 tacatgaaca tcattctgca atacgaatcc aacgccagtt cgctgtcttt agggcgcttc
    18301 gaccagtata tgttgccgtt ctatcaggca tctttaaccc agggcgaaga tccggcgttc
    18361 ctgaaagaac tgctcgaatc tttatgggtg aaatgcaacg acattgtgct gttgcgctcc
    18421 accagcagcg cgcgttattt cgcaggtttc ccgactggct ataccgcact gctcggcggg
    18481 ttaaccgaga acggacgtag cgcggtgaac gtgctttcgt tcctctgcct cgatgcctat
    18541 caaagcgtac aattaccgca accgaaccta ggggtgcgca ctaacgcctt gatcgacacg
    18601 ccgttcctga tgaaaaccgc agaaaccatt cgcctcggca ccggtattcc gcaaatcttt
    18661 aacgatgaag tggtggtgcc agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc
    18721 gactattccg tagtgggctg tgtggaatta tctattcccg gcagaaccta cggcttgcat
    18781 gacatcgcga tgtttaatct gctgaaagtg atggaaatct gcctgcatga aaatgaaggc
    18841 aatgctgcgc tgacttatga aggtttactg gagcagatcc gcgccaagat cagccactac
    18901 atcaccctga tggttgaggg cagcaatatt tgtgatatcg gccatcgcga ctgggcacct
    18961 gtaccgctgc tctcgtcttt tatcagcgat tgtctggaaa aaggtcgcga tattaccgat
    19021 ggcggcgcgc gttataactt ctccggcgta cagggaattg gtatcgccaa cctgagcgat
    19081 tctctccatg cgttgaaagg gatggttttt gatcaacagc gtttaagttt tgacgaattg
    19141 ctgtcggtat taaaagccaa ctttgcaacg ccagaaggcg aaaaagtccg cgctcgctta
    19201 attaaccgct ttgagaaata cggtaacgat atcgacgagg tgggtaacat tagcgccgaa
    19261 ctgttgcgcc actactgcaa agaagtggaa aaataccaga acccgcgcgg cggttacttc
    19321 acgccgggat cgtataccgt ttctgctcac gtcccgttgg gatcggtggt tggcgcgacg
    19381 ccagacggtc gttttgccgg agaacagttg gcagacggcg gcttgtcacc catgctgggc
    19441 caggacgcac aagggccaac agcggtactg aagtcagtca gtaagctcga taacacgctg
    19501 ctgtctaacg gtacgttgct gaacgtgaaa ttcactccgg cgaccctgga aggtgaagcg
    19561 ggattacgca aactggccga cttcttacgg gcgtttaccc agcttaagtt gcagcatatt
    19621 cagtttaacg tggtgaacgc cgacacgttg cgggaagcgc aacagcgccc acaagattat
    19681 gccgggctgg tggtgcgcgt tgccggatac agcgccttct ttgtcgaact gtcgaaggag
    19741 atccaggatg acatcatccg ccggacagcg catcagctgt aacgttgtgg aaacgcgccg
    19801 caatgatgtg gcgcgcattt tcaacattca gcgttattca ctgaatgacg gcgagggcat
    19861 tcgtacggtg gtctttttta aaggctgtcc gcatctttgc ccgtggtgtg ctaatccgga
    19921 gtcgatctcc ggcaaaatcc agacggtacg cagagaggcg aaatgtctgc actgtgcgaa
    19981 atgtttgcgt gatgcggatg aatgcccctc cggggcgttt gaacggattg gtcgcgatat
    20041 cagccttgac gcgctggaac gggaagtgat gaaagatgac attttctttc gcacgtccgg
    20101 cggcggcgtc acgctttctg gcggcgaagt gttaatgcag gcggagtttg ctacccgttt
    20161 tttacagcgg ctgcggttgt ggggcgtgtc atgcgccatt gaaactgccg gagacgcgcc
    20221 agccagcaag ctgttaccgc tggcgaaatt gtgcgatgaa gtgttgttcg atttaaaaat
    20281 tatggacgcg actcaggcgc gggatgtggt gaagatgaac ctgccacgcg tgctggagaa
    20341 tctgcgtttg ctggtgagtg agggcgtcaa cgtgatcccg cgtttaccgc tgatccctgg
    20401 tttcacgctc agccgggaga atatgcagca ggcgctggat gtactgatcc cgctgaaaat
    20461 caggcagatc catctgttac cgtttcatca gtacggcgaa ccgaaatacc gcctgctggg
    20521 gaaaacatgg tcgatgaaag aggtgcctgc gccgtcgtca gccgatgtga caacgatgcg
    20581 cgaaatggca gaacgggccg gatttcaggt taccgtggga ggttaaaatg gcatacctgg
    20641 tggcagtaac cgcctgcgta agcggcgtgg cgcatacata tatggcggcg gaacggctgg
    20701 aaaagttgtg cctgttagag aagtggggag tcagcattga aactcagggc gcgctgggaa
    20761 cggagaatcg tttagcggac gaggatatcc gtcgggcgga tgttgctctg ttgattacgg
    20821 atatcgagct tgccggtgcc gagcgatttg aacattgccg ctatgtgcaa tgcagcatct
    20881 acgcattcct gcgtgagccg cagcaggtaa tgagcgcggt gcgcaaagtg ctttctgcgc
    20941 cgcagcaaac ccatcttatt ctggagtagt cggtttttct gtcagttggc tgtggtactg
    21001 ccggcgatat tccgacggcg agcgttcggt gtttttacga aacagacggc agaagtagtt
    21061 gctgtcgaca aagccgcagg cgtgcgccac ttcttttacc ttcaggtcat agcctttcaa
    21121 taacgtctta gcgtgctcca gtcgcgtgtg attcaggtat tcgttaaaac caatggcccc
    21181 cgttttttga aacaggtgcg agagatagtt tggtgaaata tagaatgcct gtgcgacaga
    21241 ttcgcgggta agcgcggagg catagcgttc gtcgatataa tcgcgaatag cttcgaatag
    21301 tgcctggctg cgtgaggcgg tctggatttg gctgccaagc aaatcgcggc agtggctaag
    21361 caggctggcg acgataagcc ttgccgtttg ctgctcctgc ggctgcattt gcatttcatt
    21421 gagcgtttgt agcagaaaag aacctatgcg tgggccgcgt cgggcgacgt gttgcttcgc
    21481 cagattttga tattgtttgc catcccattg cacgacgctg aagccgagtt gctgtttgcc
    21541 aaacagcacg ctaaaggtag tagcaggggc ttgccattgt ggaaaattcc agccaccagc
    21601 ggcaacgtac agcacatcgc cgggaactaa cgttgctcca gcgccggtat caacaaactc
    21661 accttccagc acaatttcca gccgtggaaa atcgacctga tacgcaagat cgggcaccgg
    21721 gccgttactg ctggcaaagt aaatctggcg cagggacagc gggccgttga tcaggcggga
    21781 gagcagatag ctgacgtcgt gatacatgtt attcccttag gtatggagac gggaaaacag
    21841 taactggcat tattaaaaaa ggccacctga aaagtgtggc ctgaggggag ttcgatgtaa
    21901 acattgcggc agttgtattg ccggatgtgg cgtaaacgcc ttatccagtc taccgccgcg
    21961 atctgtaggc cggataagac gcgtcaagcg tcgcatccgg caaatagcgc ctggctgata
    22021 attactgatt acctacctgg tcgccatacg gcagcgtgtc gtaatcgatc agcgcgtttt
    22081 tcttgtacgg gttaccaatc cagcgggtat tttctttgaa ctgcggattc accactgaac
    22141 gggttggatc gtaaccgtcg taagataagc ccgctaaatc tgaccaggtg tggatcagtt
    22201 ccgcaaggct gtatttacga tcaacatcct gcgagaaatc acgcggatga gtcgcttgcc
    22261 atttttccga cgtccacagc aggaacggaa tggtgtacat atgacgcgtc gggttgtcct
    22321 cattacgccc ctgggtttta tgaggcggcg tgtcgtaaac ctcttcaccg tggtcagaga
    22381 agtaaaccag gaaaccgttc gggtctgctg ctttaaagtc tttaatcagg ctggcaacca
    22441 catgatcgtt atacaggtta gcgttgtcat aatcgttata tgactccagc tcttccgcgt
    22501 ttaatcccgg cggaacatga tcggtattgc catcaaactt gccctgattt tccgggtagc
    22561 ggtatttgta tttgatatgc gtacccagca gatgaacgat gatcagtttc ttcggcgcag
    22621 ggtccttcag cacttcctgg aacggcttca gcacgttagt gtcgtattca cgcgcactct
    22681 gcgtacgttg ctggttcatg tagtactgct tgtcggtctg gcgcgaaaat accgtcagca
    22741 tggtattgcg ggcggtcatc gtctgctggt tggtgatcca gaaggtttta taacccgcct
    22801 gtttcatcat gttcatcagc gacggctgcg tcagatacag atccgggttc ttttcattgg
    22861 caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg acgagacgta actacgttat
    22921 taaacacggt cagattcgga tcggttttat gcagcgcatt cagctccggc gtggtttcac
    22981 gcggataacc gtacagactc atgcgtccac gctgggttga ctcgccaatc accagcacta
    23041 aggtgcgcgg ttcgttaccc gattcatctt tgaaattagc cagcggcggc aaggcattat
    23101 tttcattcag tagctttgtt agcgagttta gttgctgacg atactgataa tagccggtca
    23161 ggaattgcca cggtgcggca ggctccatac gcgaggccag gttatccaac gttttctcaa
    23221 acggcttgtt tttgataaac atattcatgg cgatcggatg cagaatcaag ccataaagca
    23281 gggcaaaaga gacaacataa cgccacggct ttggaatata gaccgggcgc aggcgtgtcc
    23341 acagcagaac tgccaccgcc gtataggcca gcgcgataag cacaattttc aggctgaaat
    23401 actggcttaa atactcgctg gcttcgttgg tgttggtttc gaacatcaca aacagaacgc
    23461 tctgcgagaa ctcctgaccg tagatgacgt agtagcacag cgccgccaga gaggccgccc
    23521 atagcaccac gccgatcact gcggcaataa ttttgatccg cttcggaaag aggaataccg
    23581 gaattaacca cagcgagctg aataacagcg agtcgcgaat gccgttggtg ccactataac
    23641 cactgatgta aataatggcc tgtagcagag tagagaaaaa ccaaaagtag agcaatgccc
    23701 aacccagggc tttccagcta aaaagaggtt tagcctggac ttctgtggaa tgcatagtaa
    23761 gaacctgtct tgaaaaaaat atcgccgaat ttaccgacaa ttccttaagg atatctgaag
    23821 ggatatccag aatttgaata aaatgcagac agaaatatat tgaaaacgag ggtgttagaa
    23881 cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag aggaagatta
    23941 gccggtatta cgcatacctg ccgcaatccc ggcaatagtg accattaacg cctgctcgac
    24001 gcgaggatcc ggttcctggc cttctttttc tgcctggcgg gagcggtgca gcaactcggc
    24061 ttgcaatacg ttcagcgggt cggtgtaaat attccgtagc tgaatagact ctgcaatcca
    24121 cggcagatcg gccatcagat gggaatcgtt ggcaatcgcc agcaccactt tgatgtcttc
    24181 ttcttgcaga ttgcgtaact ctttacctaa cggccacagt gttttgtcta ccaggcgttg
    24241 gtcatagtat tccgccagcc acaggtctgc tttggcgaag accatctcca gcatgccgag
    24301 acgcgtcgag aagaatggcc aatcgcggca catagcctcc agctcgctct gtttgccatc
    24361 ttcgaccact ttttgcagcg ccgtacctgc acccagccag gcggggagca tcagacggtt
    24421 ttgcgtccag gcgaagatcc acggaatggc gcgtagtgac tcgacgccgc cggttgggcg
    24481 acgtttcgcc ggacgtgaac ccaacggcag tttgcccagt tcctgttccg gcgtagcgga
    24541 gcggaagtaa ggcacaaaat ctttgttttc acgtacgtag ccacggtaga gatcgcagga
    24601 gatgactgat agttcatcca taatgcgacg ccagctctct ttcggctcag gtggtggcag
    24661 caggttggct tccagtatcg ccccggtgta aagcgacagg ctgctgacgg tgatttctgg
    24721 cagaccatat ttaaagcgga tcatctcgcc ctgttcagtc acgcgcaggc cacctttcag
    24781 gcttcctggc ggttgtgaca gcagcgccgc atgagcaggt gcgccgccgc gaccaatgga
    24841 accgccgcga ccgtggaaca acgtcagctc aatacccgct ttttcgcagg ttttgattaa
    24901 tgcatcctgt gcctgatatt gcgcccagga cgctgccatc acgcccgcat cttttgctga
    24961 gtcggaatag ccaatcatca ccatctgttt gccctgaatc aggccgcgat accagtcgat
    25021 attcagcaac tgagtcatga catcgttggc gttgttcagg tcatcgagag tttcaaacag
    25081 cggagcaacc ggcatcgcaa acccgatacc cgcttctttc agcagcaggt ggacagccag
    25141 cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc cttgcggtgc
    25201 ttcggcaatc acctggcagg tatcgagcac ttcgcgcgtt tcggcgcttg gttgccagtt
    25261 gcgcggcaga agcggacgtt tggagttcag ttcgcggatc aggaacgcct gtttgtcggc
    25321 ctctgaccag ctttcgtagt cgccgatacc gaggtagcgg gtcagctcgc ccagcgcttc
    25381 ggtatgacgc gtgctctcct gacggatatc aatacggacc agcggtacgc cgaaacattt
    25441 cacgcggcgc agggtgtcga gcagatcgcc gttggcgata atacccatgc cacacgcctg
    25501 aagtgactgg tagcaagcgt agagcggttc ccacagttct tcgttttgtg tcagcaggcc
    25561 ttctggtttt ggcagttctt cgcctttcag gcgcgcttcc agccatgcct gtgtcgccat
    25621 caggcgagaa cgcaggtttt tcatcagata gcgatacggt tctgcggcac cttcttcgcc
    25681 aaccagcgcc agcagttcag gggtcgcttc aaccatcgac agttcagaaa ccagaacctg
    25741 aatatctttc aggaacagat cggtggcttt ccagcggctg agcagcagga cgtggcgggt
    25801 gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg cccatccacg aggtaaaacg
    25861 taccggaaca aattcgacgg gcagtttgta gccgaggttc tcttccagtt gttcgttcag
    25921 ttcgcgcagg taattcggta cgccttgcca caggctgttt tctactacgg caaagcccca
    25981 tttggcttca tctaccgggc ttggacgcag cttacggatt tcatcggtat gccatgactg
    26041 ggcgatcaac tggcgcaggc gacgcatcag ctggttgtgt tcgtagtcag cgatatcttt
    26101 gttatcgagc tgttttaagc aggcgttcac ttccaccatt ttgtggatca gtgtacgacg
    26161 ggtaatttcg gttgggtgag ccgtgaggac cagttccagc gacagcgatt ccactgcttt
    26221 tttgatggtg tcttcgctca gttccggctg gtttttcagt ttacgcaggg tgcgggcgat
    26281 cacttccggg ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt gctcggcggt
    26341 gttggccagg ttcaggaact gactaaacgc acgcgcaacg ggcagcagct cgtcgttcga
    26401 caaattttgt aaggtggtga gtaactcctg gcggttagca tcattgccag cgcgtgaaga
    26461 tttggacaac ttacggatag tttctacgcg ttcaagaatg tgttctccca acgcatcctt
    26521 gatggtttct cccagcactt tgccgagcat actgacatta ctacgcaatg cggaatattg
    26581 ttcgttcata ttaccccaga caccccatct tatcgtttga tagccctgta tccttcacgt
    26641 cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt cttttataaa gccacgtaaa
    26701 agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc aggaatttac
    26761 gtcattaaat tcacgacgct ttaaataagc gtaacttatg ggaatgttaa aaaatcgcct
    26821 caagtaacac caaaggtgta ggtcggataa gatgtgcaag catcgcatcc gacattattg
    26881 cggcactgga gtttggcaac agtgccggat gcggcgcaag cgccttattc ggcctacgtt
    26941 ttaatgccag caaaaatggt gaatgacctg ggttatcagt tcgcgggtgg gcttgataaa
    27001 ccgtgtttcc agatattcat caggctgatg agcctgatta attgagccag gccccagaac
    27061 cagcgtcggg cataacgttt gaataaacgg cgcttcggta cagtaattca ccacttcggt
    27121 ttttgctccg agcaatttct caaccacttc aaccagttga tgattcggtg ggcattcata
    27181 gccagggatc ggcggatgca gctcgtcgac cgtcagacga cccggccagc gttcgctcac
    27241 cggagccaat gcatcgttga gcaaaccatt aagttcattg agtgtcatgc caggcagcgg
    27301 acgaatatcc atatgcaact cacaccaagc gcaaatacgg ttagaagcgt cgccaccgtg
    27361 aatatgcccg aggttgagcg tagggtatgg cacggtaaac gcttcgtagt gataacgttc
    27421 tttcaggtta tcgcgcaatt gcaaaatatg cccgatggcg tcgtgcatta gttctatagc
    27481 gttaactccg cgtgctggat cgctggagtg ccccgactgg ccctgaatac ggatggcgtt
    27541 agagatatga cctttatgtg cgcgtaccgg ttgtagtgac gtcggttcgc caatgatggc
    27601 gcaatccggg cgcagggcgg tagtttcggc aaaataacgc gctccggcca tactggtttc
    27661 ttcatcagca gtcgccagaa tgtagagcgg ttttttcagt ttcgtgacgt cgacatcgcg
    27721 tagcgcatca aggataaacg caaaaaagcc tttcatgtcg gcggtgccta agccgtaaag
    27781 cttgccgtca tgctccgtca gtgtaaacgg atcgcgcgtc cagcgaccgt catcaaatgg
    27841 caccgtatcg gtatgccccg ccagcaacaa gccgccagcc ccctgtccgg tacttgccag
    27901 catattaaat ttgttgcgag ttcctggtac aggctgcact tccacattga agcctaaatc
    27961 tttaaaccag tccgccagca gagtgattaa atctgcattg ctttgatcga gtgcctcttc
    28021 cgtggcgctt attgaaggtg tggcaatcag agcacggtaa atctcgataa atggcggtaa
    28081 tttgtttttc attgttgaca cacctctggt catgatagta tcaatattca tgcagtattt
    28141 atgaataaaa atacactaac gttgagcgta ataaaaccca ccagccgtaa ggtgaatgtt
    28201 ttacgtttaa cctggcaacc agacataaga aggtgaatag ccccgatgtt gaatacgctg
    28261 attgtgggtg ccagcggcta cgctggcgca gagctagtga cctgtgtaaa tcgccatccg
    28321 catatgaaca taaccgcttt gactgtttca gcgcaaagca atgatgcggg aaagttaatc
    28381 tccgatttgc atccgcagct aaaaggcatc gttgatctgc cgttgcagcc gatgtcggat
    28441 atcagcgagt ttagccctgg ggtggacgtg gtgtttctcg ccaccgccca tgaagttagc
    28501 cacgatttag cgccgcagtt tcttgacgcg ggctgcgtgg tgttcgacct ttccggcgcg
    28561 tttcgtgtta acgacgccac cttctatgaa aaatattacg gctttaccca tcaatacccg
    28621 gaactgctgg aacaggcagc ctacggtctg gcggagtggt gcggtaataa attaaaagaa
    28681 gcgaatttga ttgcggtgcc aggctgttat ccgacggcag cacagttggc gctgaaaccg
    28741 ttgattgatg ccgatcttct tgacctcaat cagtggccgg tgatcaacgc caccagcggc
    28801 gtgagcggtg caggacgtaa ggcagcgatt tcaaatagtt tttgtgaagt tagcctgcaa
    28861 ccgtatggcg tctttaccca tcgccatcaa ccagagatcg ccacacacct cggtgctgac
    28921 gttatcttca ccccgcatct gggcaatttc ccgcgcggca ttctcgaaac cattacctgc
    28981 cgcctgaaac cgggtgtgac ccaggcgcaa gtggcgcaag cgttacagca ggcgtatgcc
    29041 cataaaccgc tggtgcggct gtatgacaaa ggcgttccgg cgctgaaaaa tgttgttggg
    29101 ctaccatttt gcgatatcgg gtttgccgtt cagggcgaac atctgattat tgtggcgacc
    29161 gaagacaact tactgaaagg cgcggcggca caagcggtac agtgcgccaa tattcgtttc
    29221 ggctatgcgg aaacgcagtc tcttatttaa gggtgcaatg atgaatccat taattatcaa
    29281 actgggtggc gtactgctgg atagtgaaga ggcgctggaa cgtctgttta gcgcactggt
    29341 gaattatcgt gagtcacatc agcgtccgct ggtgattgtg cacggcggcg gttgcgtggt
    29401 ggatgagctg atgaaagggc tgaatctgcc ggtgaaaaag aaaaatggcc tgcgggtgac
    29461 gcctgctgat cagattgaca ttatcaccgg agcactggcg ggaacggcaa ataaaaccct
    29521 gttggcatgg gcgaagaaac atcagattgc ggccgtaggt ttgtttctcg gtgacggcga
    29581 cagcgtcaaa gtgacccagc ttgatgaaga gttaggtcat gttggactgg cgcagccagg
    29641 ttcgcctaag cttatcaact ccttgctgga gaacggttat ctgccggtgg tcagctccat
    29701 tggcgtaaca gacgaagggc aactgatgaa cgtcaatgcc gaccaggcgg caacggcgct
    29761 ggcggcaacg ctgggcgcgg atctgatttt gctctccgac gtcagtggca ttctcgacgg
    29821 caaagggcaa cgcattgccg aaatgaccgc cgcgaaagca gaacaactga ttgagcaggg
    29881 cattattact gacggcatga tagtgaaagt gaacgcggcg ctggatgctg cgcgcacgtt
    29941 gggccgtccg gtagatatcg cctcctggcg tcatgcggag cagcttccgg cactgtttaa
    30001 cggtatgccg atgggtacgc ggattttagc ttaagttttg ttggccggag gcgcagcttt
    30061 ccggcattga atttcaaaat aaggaaacag agttatggca ctttggggcg ggcgttttac
    30121 ccaggcagca gatcaacggt tcaaacaatt caacgactca ctgcgctttg attaccgtct
    30181 ggcggagcag gatattgttg gctctgtggc ctggtccaaa gccctggtca cggtcggcgt
    30241 gttaaccgca gaagagcagg cgcaactgga agaggcgctg aacgtgctgc tggaagatgt
    30301 tcgcgccagg ccacaacaaa tccttgaaag cgacgccgaa gatatccata gctgggtgga
    30361 aggcaaactg atcgacaaag tgggccagtt aggcaaaaag ctgcataccg ggcgtagtcg
    30421 taatgatcag gtagcgactg acctgaaact gtggtgcaaa gataccgtta gtgagttact
    30481 gacggctaac cggcagctgc aatcggcgct ggtggaaacc gcacaaaaca atcaggacgc
    30541 ggtcatgcca ggttacactc acctgcaacg cgcccagccg gtgacgttcg cgcactggtg
    30601 cctggcctat gttgagatgc tggcgcgtga tgaaagccgt ttgcaggatg cgcttaagcg
    30661 tctggatgtc agcccgttag gttgtggcgc gctggcggga acggcttatg aaatcgaccg
    30721 tgaacagtta gcgggctggc tgggatttgc ttcagcaacc cgtaacagtc tcgacagcgt
    30781 ttctgaccgt gaccacgtgc tggaactgct ttctgctgcc gctatcggca tggtgcatct
    30841 gtcgcgtttt gctgaagatc tgattttctt taacaccggc gaagcggggt ttgtggagct
    30901 ttctgaccgc gtgacttccg gttcatcatt aatgccgcag aagaaaaacc cggatgcgct
    30961 ggagctgatt cgcggtaaat gcggtcgggt gcagggcgcg ttaaccggca tgatgatgac
    31021 gctgaaaggt ttgccgctgg cttacaacaa agatatgcag gaagacaaag aaggtctgtt
    31081 cgacgcgctc gatacctggt tggactgcct gcatatggcg gcgctggtgc tggacggcat
    31141 tcaggtgaaa cgcccgcgtt gccaggaagc ggcgcaacag ggttacgcga acgccactga
    31201 actggcggat tatctggtgg cgaaaggtgt gccgttccgc gaggcacacc atattgtggg
    31261 tgaagctgtg gtggaagcca ttcgtcaggg caaaccgctg gaggagctgc cgctcaccga
    31321 gttgcagaaa tttagtccgg tgattggtga agatgtctat ccgattctgt cgctgcaatc
    31381 gtgcctcgac aagcgtgcgg caaaaggcgg cgtctcacca cagcaggtgg cgcgggcgat
    31441 tgcttttgca agggcgcggt tggggtaaga acatttatat gtacaaattt gagcctggct
    31501 tatcgccggg cttttttatg gcaaaaaaaa gcggatcctg gagatccgca aaagttcacg
    31561 ttggctttag ttattcgagt tgagaaactc tcgaaacggg cagtgacttc aagggttaaa
    31621 agaggtgccg ctccgtttct gtgagcaatt atcagtcaga atgcttgata gggataatcg
    31681 ttcattgcta ttctacctat cgccatgaac tatcgtggcg atggaggatg gataatgaat
    31741 attcgtgatc ttgagtacct ggtggcattg gctgaacacc gccattttcg gcgtgcggca
    31801 gattcctgcc acgttagcca gccgacgctt agcgggcaaa ttcgtaagct ggaagatgag
    31861 ctgggcgtga tgttgctgga gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg
    31921 ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga aagtccttaa agagatggca
    31981 agccagcagg gcgagacgat gtccggaccg ctgcatattg gtttgattcc cacggttgga
    32041 ccgtacctgc taccgcatat tatcccgatg ctgcaccaga cctttccaaa gctggaaatg
    32101 tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat
    32161 tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat
    32221 gagccaatgt tgctggctat ctatgaagac cacccgtggg cgaaccgcga atgcgtaccg
    32281 atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat
    32341 caggcaatgg gtttttgctt tgaagccggg gcggatgaag atacacactt ccgcgcgacc
    32401 agcctggaga cgctgcgcaa catggtggcg gcaggtagcg ggatcacttt actgccagcg
    32461 ctggctgtgc cgccggagcg caaacgcgat ggggttgttt atctgccgtg cattaagccg
    32521 gaaccacgcc gcactattgg cctggtttat cgtcctggct caccgctgcg cagccgctat
    32581 gagcagctgg cagaggccat ccgcgcaaga atggatggcc atttcgataa agtgttaaaa
    32641 caggcggttt aaaccgttta acgcagctac ccgataggct tccgccatcg tcgggtagtt
    32701 aaaggtggtg ttgacgaagt actcaatagt gttgccgcca cctttctgtt ccataatcgc
    32761 ctgaccgata tgaataattt cggcagcgcg ctcgccaaag cagtgaatgc ccagaatctc
    32821 ttttgtttcc cgatggaaca aaattttcag cgtgcccacg ttcatgccga cgatttgtgc
    32881 acgtgccaga tgtttgaact gggcgcggcc cacttcatat ggcactttca tcgcggtcag
    32941 cagctgttcg gttttgccca cagagctgat ttccgggatg gtgtaaatgc cggtagggat
    33001 atcttcaatc agatgcgcgg tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc
    33061 ctggtcatag gccgctgatg ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac
    33121 gtgcggctgt gcggtctgat acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc
    33181 cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg gtacgaccgt tggcatagag
    33241 caggcagtca gctttcagtt ttttacccga cttcagatgc atgatcacac cgtcgtcaca
    33301 gccttcgatc ttctcgtact cttcgttgtg acgaatcact acgccactgt tccagaagtg
    33361 ataggagaga gaatctgaca tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt
    33421 gatcagatcc acttttacat ccataccgcg gaagatcgac gcatattcac agccgatcac
    33481 tccagcacca tagataagta catggcgcgg ttcgtggtgc atgctgagaa ttgagtcgct
    33541 gtcgtaaatg cgtggatggg tgaaatcaac atctgttgga tgatatggac gagagccgca
    33601 ggcaataaca aatttttcag cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc
    33661 caacgtatgc tcgtcaacaa agcgagcgtt cccctgcaat atttcacagt gattacgttc
    33721 gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg ttatcggcat ggttaaggat
    33781 atcggcaaaa gaagagcgga gcagtcggga atggtcgctg tatagtgggt tttgattgaa
    33841 ttctataatg cggctgacgg cgtgacggag agctttcgac gggatggtgc cccagtgggt
    33901 gcaaccgccg ccaacatttt gataacgctc gataactgcg acgcgcgcac cttgcttaac
    33961 caggcccatt gcagcgcctt cgccgccggg gccggaacct attactatgg catcgtaatc
    34021 gtaggaatgt ggcatggtag ggcttacctg ttcttataca taaaagcaac agaatggtaa
    34081 cattttatcg cgggtaagcc aattgatccc cgtcattaat ctggctatag cgtgagcggc
    34141 ctttgctttg tctgcctcct tcttaaccca tttattcact attaatcgct ttttattatg
    34201 gctg
//
LOCUS       DAZFKY010000030        33311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFKY010000030 DAZFKY010000000
VERSION     DAZFKY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(76..1341)
                     /locus_tag="ACHKCL_001297"
     CDS             complement(76..1341)
                     /locus_tag="ACHKCL_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5762301.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHKCL_001298"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHKCL_001298"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKCL_001299"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHKCL_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5762302.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHKCL_001300"
     CDS             2580..2663
                     /locus_tag="ACHKCL_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762303.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHKCL_001301"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHKCL_001301"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5762304.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKCL_001302"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHKCL_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5762305.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKCL_001303"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHKCL_001303"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5762306.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKCL_001304"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHKCL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5762307.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKCL_001305"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHKCL_001305"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5762308.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKCL_001306"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHKCL_001306"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5762309.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKCL_001307"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHKCL_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5762310.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKCL_001308"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHKCL_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5762311.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKCL_001309"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHKCL_001309"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5762312.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKCL_001310"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHKCL_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5762313.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQATASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13574..14710)
                     /gene="hemW"
                     /locus_tag="ACHKCL_001311"
     CDS             complement(13574..14710)
                     /gene="hemW"
                     /locus_tag="ACHKCL_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5762314.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14703..15296)
                     /gene="rdgB"
                     /locus_tag="ACHKCL_001312"
     CDS             complement(14703..15296)
                     /gene="rdgB"
                     /locus_tag="ACHKCL_001312"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5762315.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15304..15594)
                     /gene="yggU"
                     /locus_tag="ACHKCL_001313"
     CDS             complement(15304..15594)
                     /gene="yggU"
                     /locus_tag="ACHKCL_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5762316.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15591..16157)
                     /gene="yggT"
                     /locus_tag="ACHKCL_001314"
     CDS             complement(15591..16157)
                     /gene="yggT"
                     /locus_tag="ACHKCL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5762317.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16175..16879)
                     /gene="yggS"
                     /locus_tag="ACHKCL_001315"
     CDS             complement(16175..16879)
                     /gene="yggS"
                     /locus_tag="ACHKCL_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5762318.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16897..17877
                     /gene="yggR"
                     /locus_tag="ACHKCL_001316"
     CDS             16897..17877
                     /gene="yggR"
                     /locus_tag="ACHKCL_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5762319.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18068..18484)
                     /gene="ruvX"
                     /locus_tag="ACHKCL_001317"
     CDS             complement(18068..18484)
                     /gene="ruvX"
                     /locus_tag="ACHKCL_001317"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5762320.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18484..19047)
                     /gene="yqgE"
                     /locus_tag="ACHKCL_001318"
     CDS             complement(18484..19047)
                     /gene="yqgE"
                     /locus_tag="ACHKCL_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5762321.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19159..20106)
                     /gene="gshB"
                     /locus_tag="ACHKCL_001319"
     CDS             complement(19159..20106)
                     /gene="gshB"
                     /locus_tag="ACHKCL_001319"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5762322.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20119..20850)
                     /gene="rsmE"
                     /locus_tag="ACHKCL_001320"
     CDS             complement(20119..20850)
                     /gene="rsmE"
                     /locus_tag="ACHKCL_001320"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5762323.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20930..21637)
                     /gene="endA"
                     /locus_tag="ACHKCL_001321"
     CDS             complement(20930..21637)
                     /gene="endA"
                     /locus_tag="ACHKCL_001321"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5762324.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21732..22229)
                     /gene="yggI"
                     /locus_tag="ACHKCL_001322"
     CDS             complement(21732..22229)
                     /gene="yggI"
                     /locus_tag="ACHKCL_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5762325.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22306..23700)
                     /gene="galP"
                     /locus_tag="ACHKCL_001323"
     CDS             complement(22306..23700)
                     /gene="galP"
                     /locus_tag="ACHKCL_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5762326.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWIVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24137..25291)
                     /gene="metK"
                     /locus_tag="ACHKCL_001324"
     CDS             complement(24137..25291)
                     /gene="metK"
                     /locus_tag="ACHKCL_001324"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5762327.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25347..25598
                     /gene="yqgD"
                     /locus_tag="ACHKCL_001325"
     CDS             25347..25598
                     /gene="yqgD"
                     /locus_tag="ACHKCL_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5762328.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25709..25852
                     /gene="yqgG"
                     /locus_tag="ACHKCL_001326"
     CDS             25709..25852
                     /gene="yqgG"
                     /locus_tag="ACHKCL_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5762329.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            25946..26077
                     /gene="yqgB"
                     /locus_tag="ACHKCL_001327"
     CDS             25946..26077
                     /gene="yqgB"
                     /locus_tag="ACHKCL_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5762330.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26086..28062
                     /gene="speA"
                     /locus_tag="ACHKCL_001328"
     CDS             26086..28062
                     /gene="speA"
                     /locus_tag="ACHKCL_001328"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5762331.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            28208..28939
                     /locus_tag="ACHKCL_001329"
     CDS             28208..28939
                     /locus_tag="ACHKCL_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5762332.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            29075..29995
                     /gene="speB"
                     /locus_tag="ACHKCL_001330"
     CDS             29075..29995
                     /gene="speB"
                     /locus_tag="ACHKCL_001330"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5762333.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(30201..30959)
                     /gene="loiP"
                     /locus_tag="ACHKCL_001331"
                     /gene_synonym="yggG"
     CDS             complement(30201..30959)
                     /gene="loiP"
                     /locus_tag="ACHKCL_001331"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5762334.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            31237..33228
                     /gene="tkt"
                     /locus_tag="ACHKCL_001332"
     CDS             31237..33228
                     /gene="tkt"
                     /locus_tag="ACHKCL_001332"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5762335.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         8072 a         8376 c         8783 g         8080 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggtga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 acagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaataca agtttactca gccagaaaaa gctccagcag cgaatttaaa aacagcttcc
    13621 catgttccgt aatctgccag taatccgcac attcggtgag ataaccctgg gcaatcgcct
    13681 cgtctaactg tgggcgaatc acatcttcgc aaagcccggt atacgcacta aactccacgc
    13741 gcggcgcagg ttccagcaac cggaagcgat tcataaagaa ctcaaacggc ttatctgcgg
    13801 cttcgacatc acgctggctt tccagatacc ttccttgcat aaaaccacgc ggatgacgcg
    13861 ttttggtggt acgcagaatg cgcccatccg ggaaggtcac tttgccgtgc gcgccgcagc
    13921 caataccgat gtagtcacca aagcgccagt agttgagatt gtgctggcac tgataaccgg
    13981 gtttggcgta agcggaagtt tcatattgct gataacccgc tgcggttaat aactgatgcc
    14041 cctgttcgaa aatatcccac agcgcgtcgt cgtccggcag taccggcggg cgtgaaccaa
    14101 acagcgtatt aggttcgatg gtcagttgat accaggaaag atgcggcgga ttcagttcaa
    14161 tagcctggcg cagatcgcca agcgcctctt ccagcgattg atccggtagc ccatgcatca
    14221 aatcaaggtt aaagctacgt aaccctaaac cgctcgccag ctttgccgct cgtttcgctt
    14281 cttgcgggcc atgaatacgt cccagccgtt tcagcttttc ttcgctaaaa ctctgtaccc
    14341 caatagagat acgatttaca ccggcacgct gataatcaac aaagcgatcg gcttctaccg
    14401 taccggggtt cgcttccatg gtaatttctg catccgctgc cagcggcaaa cgcgcacgca
    14461 cgccgtccag cagcgtttgc atcgccgagc cggaaagcag gctcggcgta ccaccgccaa
    14521 taaagattgt ctttacttca cggccctgag cgtaagccac atcgttgtcc agatcgttaa
    14581 gcagatgctg aacgtaatcg tcgtgcggca cttctccttt caacgcgtga gagttgaaat
    14641 cgcagtacgg gcatttctgc acgcaccacg ggatgtgaat gtagagactc agcggcggta
    14701 atttaaccat tacgtaaagc gtccagcagc agtttcaatg cctgaccacg gtgggaaatg
    14761 gcgctctttt cttcgcgggt cagttcggca gcggttttcc cttcggaagg tacgaagaag
    14821 attggatcat aaccaaagcc accagtgccc gccggttcgc gagtaatcac acccggccag
    14881 ctgccatggc acaccagcgg ggtcggatct tctgcgtgac gcagatacac cagcacgcag
    14941 tggaaacgcg cctgacgttg gtcgtccggt acgtctttca gtgtttccag cagtttttgc
    15001 agattctttt gatcggtcgc gtcttcaccg gaataacgtg cggagtaaat acccggcgcg
    15061 ccgccaagca catctaccgc caggccagag tcgtcggcaa ttgccggtaa accggtcact
    15121 tttgccgcgt ggcgcgcttt cagaatcgcg ttttcgataa aggtcaggcc ggtttcttca
    15181 gcggaatcaa cgccgagttc tgtttgggcc acgatatcaa gaccgaagtc gctaagcagc
    15241 gacgccagct cacgcacttt accggcattg ccggttgcga ggacaacttt ttgcatagga
    15301 tacctaatta attaacgccg cgacttctgg cgggatttgt tgcggattaa tgattttaat
    15361 ttgtttgtgg cggccaagtt cgcctttttc aatcaccacc tggcttttgg caacccggaa
    15421 ttgcttaccg agaaacttca ccagatggct gttggcctga ccgtcaaccg gcggcgcggt
    15481 aatggcgact tttacctcgt cgccatgtaa accgacaata gaatcacggc tggctttcgg
    15541 ctgaatatag agccgtaaaa ccagaccgtc atcgttaact gttacggcac tcataacgcc
    15601 atccacagcc ccggcagcag catatttccg gtcgcctgta atacttctgc gacacccata
    15661 ttgatgacat acagcagcag aacgaggatc atcggcgaga aatcaatccc ccccattgcc
    15721 ggtagcaagc ggcgaatcgg gcgcagcagc ggatcggcca gctgaatcag cacgtattca
    15781 atcgggctac ggccctggct tacccagctc ataatcgcca tcaccagcag gacccagaaa
    15841 atcagcaggc cgatggtttt cagcagaatc agtaaaccgg caatccagat gattggcagg
    15901 aaggtcacca ctttaaacag cacgatggct ttgataaaac tgagaatata ggcaaccagc
    15961 agcgaggcgc tatcaatggg tcccattgcc ggaataacgc ggcgcagtgg cccgataatt
    16021 ggctgcgtta ccttcactac aaactgtgag aaggggttgt aaaaatcaca atgagcccac
    16081 tgcatccaga tgcgtaataa cagcaccatg gtatacagct caatgaccgt tgaaagcagg
    16141 aaagtcaacg tattcatggc gttcctttaa ttccttattt tttagagtaa tcacgcgcac
    16201 caaaaattgc agtgccgata cgaaccatcg tgctacctgc cgcaatggcg gcttccatat
    16261 cgtccgacat tcccagagag agcgtgtcga tatgcgggta gcgcgttttc agtccggcaa
    16321 atgctacagc catttggcgt gcaacttcaa actgccttac atattctgac tcaggcgcag
    16381 ggattgccat cagcccgcgc agacgtaaac gcggtagttc agcgaccgca gctgccagct
    16441 cgtccagttc tgccagttga atcccggact tactgttttc atcactaata ttaatttgaa
    16501 tcagtacgtt aagaggggga agttctgccg ggcgctgatc gttgaggcgg gtagcaatgc
    16561 gcaaacggtc gatggtatga caccagtcga aatgctctgc caccaggcgg cttttattag
    16621 actgcaacgg gccaataaaa tgccattcta atcctgttac gcccagttcc tgaaagtggc
    16681 gaattttatc taccccttcc tgaacgtagt tttcaccaaa ttgacgctgc ccggcatcaa
    16741 tggcttctgc gatggcgctc gcaggttttg ttttactgac tgcaagcagc gtaatttctt
    16801 ctggagaacg gccgcaacgc gttgcagccg ctgagatttt gtcccggacc tgtgccaggt
    16861 tatgcgcaat atcgttcatt ttccgaggat cttagtatga atatggaaga aattgtggcc
    16921 cttagtgtaa agcataacgt ctcggatcta cacctgtgca gcgcctggcc cgcacgatgg
    16981 cgcattcgcg ggctaatgga agctgcgccg tttgatgcgc cggacgtcga agagctactg
    17041 cgggagtggc tggatgacga tcagcgggca atattgctgg agaatggtca gctggatttt
    17101 gccgtgtcgc tgacggaaaa ccagcgattg cgtggcagcg cattcgcgca acggcatggc
    17161 atttcgctgg cgttacggct gttaccttcg cactgcccgc agctcgaaca gcttggcaca
    17221 ccaccgatat tgccggagtt actcaagagc gagaatggct tgattctggt gacgggggcg
    17281 acggggagtg gcaaatctac cacgctggcg gcgatggttg gttatctcaa tcaacatgct
    17341 gatgcgcata ttctgacgct ggaagatcct gtggaatatc tctatgccag ccagcgatgt
    17401 ttgatccagc agcgggaaat cggtttgcat tgtatgacgt tcgcatcggg attgcgggcc
    17461 gcattgcggg aagatcctga tgtgattttg ctcggagagc tgcgtgacag cgagacaatc
    17521 cgtctggcgc tgacggcggc agaaaccggg catctggtgc tggcaacctt acatacacgt
    17581 ggtgccgcgc aggcagttga gcgacttgtg gattcatttc cggcgcagga aaaagacccc
    17641 gtgcgtaatc aactggcagg tagtttacgg gcagtgctgt cacaaaagct ggaagtggat
    17701 aaacaggaag gacgcgtggc gctatttgaa ttgctgatta acacacccgc ggtggggaat
    17761 ttgattcgcg aagggaaaac ccaccagtta ccgcatgtta ttcaaactgg gcaacaggtg
    17821 gggatgataa cgtttcagca gagttatcag cagcgggtgg gggaagggcg tttgtgacgt
    17881 tgccatgccc gatgcgcaag cttatcgggc ctacaaaggc aataaacgtc tgatgcgacg
    17941 cttgcgcgtc ttatcagacc tacaaccgct gccacatgta ggccggataa ggcgttcacg
    18001 ccgcatccgg caataaggcg taaggcgcga tgatattatt tacactccag caggcgatta
    18061 aatctcctta atatccctgc tcgaaatagc tttcgagaat aataaccgca gaggcagagt
    18121 cgattttgcc tttgttgagc gcccgatagc cgccctgttc aaacagaccg gaacgtgcct
    18181 ccacggtgct aagacgctcg tcatggagct ttacttcaac accgaaacga ccatgaatac
    18241 ggttagcaaa tttacgcgct ctggcggtta acggctgttc ggtgccgtcc atattcagcg
    18301 gtaaaccgac gatgatttcg tctggctgcc actctttcag taaacgttcg atgaggttcc
    18361 agtccggcgt accgtcctgt gctttaattg caggcaaagg gcgagcggtg ccggtaatgc
    18421 gttggccgac cgctacgcca atgcttttgg tgccgaagtc gaaggcgagt aaggttccac
    18481 tcatcaggcg tgtcctgcca caccaggcat ggtgagaata tccacaccaa tcagttttgc
    18541 cgcctcgcgc cagcggtcgg caattggcgt tttgaacaga atattcagat ctgccggggc
    18601 ggttagccac gcgttatcga gaatttcttg ttccagttga cctttttccc aggaggcata
    18661 gcccagagcc accaatacgt cagacggttg tttatcggtg ccgagcgttt ccagcacatc
    18721 gcgggaagtg gtcatcactg tgttgtctga aatgcgaatg ctggaagcaa aattggaggg
    18781 cggagtatgc aaaataaacc cgcgatcttc agccagtggg ccgccgagca taaccggttt
    18841 atccagacgg attgattcat cacgcggctc cggcgtgatc ttcagctttt ccagaatccc
    18901 ttcaattttg agattttcca gcggcttgtt gacgatgatc cccattgcac cattggtatt
    18961 atgttcgcaa atgtagacca cggaacgacg gaaaatcgga tcctggagag caggcatggc
    19021 aataagaaag tgatgctgta aattcattgt cagaggttct gtttcctggt tcaaaaaagc
    19081 aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact cagagtctca acgagatcct
    19141 tctcgctaag gtgggttact actgctgtaa acgtgcttcg atggcatcca ttaacattcc
    19201 ggtgatcgac accggaaact ctgcttcaat ctcacgaata caggttgggc tggtgacgtt
    19261 aatttcagtc agacggtcgc cgatgatatc cagaccaaca aaaatcagcc ctttttcttt
    19321 cagcgtcggc ccgatctgac gggcgatttt ccagtcactt tccgtcagcg gacgaggttc
    19381 accgcgacca ccggcagcca gattgccacg ggtttcgccc ccctgcggaa tacgcgccag
    19441 gcagtacggt actggttcgc cgtccaccac cagcacgcgt ttgtcgccat ctttaatggc
    19501 tggcagataa ttttgtgcca tgcagtagcg agtgccgtgt tcggtcaagg tttcggcaat
    19561 gacgccgagg tttggatcgc cttctttcac gcggaaaatc gacgcgccgc ccataccgtc
    19621 cagcggctta agaatgatgt cgctgtgttt ctcccagaac gcttttagct gcgctttatt
    19681 gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag gcggtaaaca gtttctcgtt
    19741 acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc cctttctctt cggcacgttc
    19801 cagaatatag gtcgcgtaga taaactcggt atcaaacggc gggtctttac gcatcaggat
    19861 cacatcgaga tcggccagcg gcagatcctg ttcaccgacg aatgaaaacc actcttcgta
    19921 gttctgtttc acgttcagcg tgcgggtatg ggcgcgggct tcgccattga tcagatacag
    19981 atcgcccatc tccatatagt gaagttcgta accacgacgc tgtgcttcca gcaacatagc
    20041 gaaactggaa tctttcttga tgttgatgtt tgcgatgggg tccatcacga tgccgagctt
    20101 gatcattatt cttctccgtt agcccaaatc gccaaatcgt acttgtagcg cggtaatggc
    20161 ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc aacaggatat cagtaaattg
    20221 atagcgggca gtcatggcaa tttcatctgc cgataaaccg ccttccgggc caatcagcag
    20281 gcggacgcgt tcaaccggta aaggcaacgt attgatgctg ttactggcgc gcgggtgaag
    20341 attcagtttc agtccttcat cctgctctgc acaccaggct tccagatcca tcgctggacg
    20401 gatttccggc acccggttac gaccacactg ctcacaggca gcaattgcaa tcttctgcca
    20461 ctgctggagc ttcttgttca gacgttcact atccagttta acgccgcagc gctcagaaaa
    20521 aagtggcgta atgaggctta caccgagttc gatcgatttc tggatcgtaa attccatttt
    20581 ttcaccacgc gacatcacct gaccgaggtg aatatgcagc ggagattcgc gatcgtcgat
    20641 ctggccttcc agcaccttca cttccacgct ttttttgctg gcgctggtaa tttcggcgtc
    20701 aaagacctgg ttgctgccgt caaacaattg caacgcctgt cccggcccca tacgcagtac
    20761 gcgcccaata tggttggcgg catcttcgca aagcgcgatg tgagaatggc tggtcagtgg
    20821 ttcaggatga taaatgcggg ggatacgcat agttaaaaat ccgcgtcgtc tccccacgcg
    20881 gttgtacgcg tggggtaggg gttaacaaaa agaatcccgc tagtgtaggt tagctctttc
    20941 gcgcctggca agcgcgttgc acatacgggt tatgattgcc ctgtaccttc gcgatgcgtt
    21001 catcgcgctc gcactcccag tcggtaaccg gatacatctt gtcccatgcg ttgaacagct
    21061 gcgtttgctg gcgagagagt gtcaggttgt aatggtcacg catatagaag taggtgcgcg
    21121 caatggcacc gcgtgcacgc gctggtggtt cggcagcttt ttctttgaaa tcgaccttca
    21181 tggcgcattg accgtactgg ccttcaccgc cattccactg gctgtacata aagttgccgc
    21241 gatcgccatt cacctcgccg actgacggtt gcaggttatg catatcgctt tccatcttgc
    21301 gatagaccgg atctttggcg caatttttac gtccaccgtc ctgccagcac tggcgctggt
    21361 gaccgaattg ccaggcggga acgacgtgtt cccactctac gcggctggcg cggttttcat
    21421 ttttgcgcac ctgatagccg cacgattgca gatcgacaac gccttttttg ccctgccagt
    21481 taattttaca tccgcaataa aacgtaccgg gcgcgtcagc gtggactttt accgccgcgg
    21541 ctttcgcctg agaaaaacta ttgatacctt cggccaacgc cgggccggaa aatgctgcgc
    21601 tcagtaccac cgcagcaata gacaaataac ggtacatcac ttactccgtg ttaaaacgag
    21661 ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc agatcaggaa agttcctgat
    21721 agttcagatg gttattttgc gaccagttgt tcgccgcagt gaacacagcg atagacggct
    21781 tcgccacgaa ctacgcgatt atgacggcgt acggtaagct gatgttcctg acacttacag
    21841 ctgtagggga aggtgttgcg gcgcacggat tgcagttcga actgatgcgt acggcgggct
    21901 ggaacgccca gcacactttc catcatccac ttccactctt tgccatgtgg tgctacgcgg
    21961 ccaaaatgtt tccataccag caaatgtgcc agttcgtgcg gtaccacttc ttcaataaag
    22021 gcttcactgt tttccaacag caaaacggga ttaaggcgaa tttcatagct ttccagccag
    22081 gctgttccgg cagaggttcc gcgctgggtg taagagagtt ttggctccgg gtagttacgc
    22141 cccaatttca ggttggcctg ggcgagtttt tcccgcaggc gacgcataac ggcctgctgg
    22201 atggcgatag ggagacggga tgttttcata gcggcagagg atagagcgaa gaaaagatga
    22261 ctgcaagagg tggcttcctc cacgacggga ggaagcctgg ggagattaat cgtgagcgcc
    22321 gatttcgcgc agtttacgac ctttcatcag attacgttca atatgttcca gcgaaacgtg
    22381 tttggtttct ggcaccagcc acagtgtcag caggataaac aggacgttca gacccgcgta
    22441 aacccagaag gtattagcgt tgcccagcgt gttgagcatg gtcaggaacg ttgcgccaac
    22501 gatcatgttg gcaatccagt tggtggcggt ggagcaggtg atgccaaaat cgcggccttt
    22561 cagcggctga atttcggagc acagtaccca aatcagcgga ccggcactca tggcaaaacc
    22621 gacaataaac atcagcagca tggcgatggc gaaatactgc gccgacggag agtggatacc
    22681 gatatgcatc attgtaccga gtacgcccat accagcagcc atcaccagga agcccagcgt
    22741 tagcgttggt ttacgtcccc agcggtcaac aaggccgatt gcgataaagg tggcaagtac
    22801 gttggtcagg ccgacaatca ctgtccccca catttgctcg gtggtgttgg tataacccgc
    22861 cagttcgaag attttcggcg cgtaatacat gatgacgttc atcccggtga attgctgcat
    22921 tacctgtaac agtacgccaa ggaacaccgc gcggcggaag ttgctattct ctttaaacag
    22981 cgcccagcca ctctgtttaa cctgcaaact ttcacggatt tcatccagtt cgcgtttcgc
    23041 ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg gcatcaacaa aacggcgttt
    23101 ggcggcaaac caacgtgggc tgtctggcag gaagaagaca ccaatcagca gcaaaattgc
    23161 cgggatgata atcacaccca gcatccagcg ccatgcaccg gtgtagctga aggcggtatc
    23221 agaaagataa gcaccgagga tcccgattgt gatcattaac tgatacatcg agatcatact
    23281 gccgcgaatt ttttccggcg caatttcaga gaggtacagt ggtgcggtat aagaggccac
    23341 acctaccgcc agccccagca gaacgcggga aagaatcagt acttcaacgt ttggcgcagc
    23401 cgcagagaac agcgaaccgg caacaaacaa aattgcgccg atcatcaggc tctttttgcg
    23461 cccgagttta aaggagagcc agccgctgcc caccgcaccg actgccgcac cgaacatcat
    23521 ggagcttacg atccattctt gcgtgtgcga agtaatctgg aattcatctg caataaacgg
    23581 cagtgcgcca gcaattacac cgatatccag gccaaagagt aatcccgcca gagcggcaag
    23641 gaagcagacg aaaaacgtca ttgccttgtt tgactgcccc tgttttttag cgtcaggcat
    23701 gatgccctcc aatatggtta ttttttattg tgaattaaga taggtgagta cgacgtaaaa
    23761 agatgtgaag caaatcacat cagtgtaatc gcttacacta atatttatct tagttatgat
    23821 ttaagtgcat gaaaataatt gagttatatc atcatagatt aaacgctgtt atctgcaatt
    23881 aagactttac tgaaaagaaa tgtaacaact gtgaaaaccg aataataata tggaaatatt
    23941 acaaatcttt gtctgttgtg gttggtgtaa tcggtttcag ttgtgatgat aatttgcggt
    24001 gaaacgtgat caggaagaat gatgttattg tgcaggcggt ttttttgccg gatgcggcgt
    24061 gaacgcctat ccaccgcggt gaggtaaaag aaaaaggcca gcgcgaggct ggcctttgaa
    24121 agcaggtgaa gaaagattac ttcagaccgg cagcatcgcg cagcagctgc gctttgtcgg
    24181 ttttttccca cgggaaatgt tcacgaccaa agtgaccgta tgctgcggtt tctttgtaga
    24241 tcgggtgcag cagatccagc atctgaatca gaccgtatgg gcgcaggtcg aagaactcac
    24301 gtaccagcag agtcagttgt tcagaaggca ctttctcagt accgaaagtt tctaccatga
    24361 tggaagtcgg ttcagccacg ccgattgcgt aggaaacctg aatttcacaa cgatcggcca
    24421 ggccagcagc aacgatgttt ttcgcgacat aacgtgctgc gtaggctgcg gaacggtcca
    24481 cttttgatgg atctttacca gagaatgcac cgccaccgtg acgcgccatg ccgccgtagg
    24541 tatcaacgat aattttacga ccagtcagac cgcagtcacc catcgggcca ccgataacaa
    24601 aacgaccggt cgggttgatg aagaatttgg tggcagaagt cagccattca gcgggcagaa
    24661 tcggcttgat gatctcttcc attaccgctt cttgcagcga tttctggtcg atctcttcag
    24721 agtgctgagt ggaaagcacg acagcatcga taccaacgat tttgccgtcg tcatactgga
    24781 aagtcacctg gcttttcgcg tccgggcgca gccacggcag agtgccgttt ttacgcactt
    24841 cagcctgacg ctgtaccaga cggtgtgcat aggtgatagg tgctggcatc agcacgtcgg
    24901 tttcattagt tgcgtagcca aacatcagac cctggtcacc cgcgccctgt tccagcggat
    24961 cggcacggtc aacgccctgg ttgatgtcag gagactgttt gccgatagcg ctcagaactg
    25021 cacaggagtt agcgtcaaag cccatgtcgg aatgcacata gccaatttcg cgaacggtgt
    25081 tacgggtgat ctcttcgatg tctacccagg cgctggtggt gatttcgccg ccaactaaaa
    25141 ccatgccggt ttttacgtag gtttcgcaag caacgcgtgc tttcggatcc tgttcgagga
    25201 tcgcgtctaa aacggcatca gagatttggt cagcaatttt gtcaggatgc ccttcagaga
    25261 cggactcgga cgtaaaaagg tgttttgcca tatttaatat cacctaaaga gaatttggtt
    25321 agctcaaact gttgtgtgga ttttctgtgg tagcggatcc taccacgact ctgcaggtta
    25381 aaaacactgg cagtctgagt gttaatcggt atggatggat taacatctgg atggctattt
    25441 taggtcaatt cttcacccga tttccacttt tttttgaatc gtgtctcatt ctgttaaaaa
    25501 cgtggctgga aatttttcct gacaatgccg gcattctgcg tatttatctt ttgcaatttt
    25561 ctgccattgt ggggtataaa acgcggcgcg cggcttaaat aaaaagcaca cgacgtttct
    25621 ttcgtgttgc cacttccagc cgggttgaaa tcagagtttt ggcttgtggg ttcgtcttaa
    25681 caggcggccg tggaggtgat acgaaataat gaaccgttgt ctgctgctta acctgtctca
    25741 ccgttctggt gaagattcgt tccccgcact ctgcatctct gctttgcata cctgccgatg
    25801 ttatacccat ctcggcgctt ctcaggattc aagagctggt tacagttact gaggactgaa
    25861 caagggcgct cttgtaaaaa caagagtttt ctcgtggttt cgccgaactg tcacacttac
    25921 gttcggttat gtgcttaata atgttatgaa aaagaaaccg gttgcgcagt tggagcgtca
    25981 gcattcactg ctggaaaatc catgtgctta tgggttgtta tcgcagttcc aggctgcgat
    26041 agtcgttaac tgttttacac ttaataaaat aatttgaggt tcgctatgtc tgacgacatg
    26101 tctatgggtt tgccttcgtc agcgggcgaa cacggtgtac tacgctccat gcaggaggtt
    26161 gcaatgagct cccaggaagc cagcaagatg ctgcgtactt acaatattgc ctggtggggc
    26221 aataactact atgacgttaa cgagctgggc cacatcagcg tgtgcccgga cccggacgtc
    26281 ccggaagctc gcgtcgatct cgcgcagtta gtgaaaactc gtgaagcaca gggtcagcgt
    26341 ctgcctgcac tgttctgttt cccacagatc ctgcagcacc gtttgcgttc cattaacgcc
    26401 gcgttcaaac gtgcgcggga atcctacggc tataacggcg attacttcct tgtttatccg
    26461 atcaaagtta accagcaccg tcgcgtgatt gagtccctga ttcattcggg cgaaccgctg
    26521 ggtctggaag ccggttccaa agccgagttg atggcagtgc tggcacatgc tggcatgacc
    26581 cgtagcgtca tcgtctgcaa tggttataaa gaccgcgaat atatccgcct ggcattaatt
    26641 ggcgagaaga tggggcacaa ggtctatctg gtcattgaga agatgtcaga aatcgccatt
    26701 gtgctggatg aagcagaacg tctgaatgtc gttcctcgtc tgggcgtgcg tgcacgtctg
    26761 gcttcgcagg gctccggtaa atggcagtcc tccggcgggg aaaaatcgaa gttcggcctg
    26821 gcggcgactc aggtactgca actggttgaa accctacgtg aagccgggcg tctcgacagc
    26881 ctgcaactac tgcacttcca cctcggttcg cagatggcga atattcgcga tatcgcgaca
    26941 ggcgttcgtg aatccgcgcg tttctatgtt gagctgcaca agctgggcgt caatattcag
    27001 tgcttcgacg tcggcggcgg tctgggcgtg gattatgaag gtactcgttc gcagtccgac
    27061 tgttcggtga actacggcct caatgaatat gccaacaaca tcatctgggc gattggtgat
    27121 gcgtgtgaag aaaacggtct gccgcatccg acggtaatca ccgaatcggg tcgtgcagtg
    27181 actgcgcatc acaccgtgct ggtgtctaat atcatcggcg tggaacgtaa cgaatacacg
    27241 gtgccgaccg cgcctgcaga agatgcgccg cgcgcgctgc aaagcatgtg ggaaacctgg
    27301 caggagatgc acgaaccggg aactcgccgt tctctgcgtg aatggttaca cgacagtcag
    27361 atggatctgc acgacattca tatcggctac tcttccggca cctttagcct gcaagaacgt
    27421 gcatgggctg agcagcttta tttgagcatg tgccatgaag tgcaaaagca gctggatccg
    27481 caaaaccgtg ctcatcgtcc gattatcgac gagctgcagg aacgtatggc ggacaaaatg
    27541 tacgtcaact tctcgctgtt ccagtcgatg ccggatgcgt gggggatcga ccagttgttc
    27601 ccggttctgc cgctggaagg gctggatcaa gtgccggaac gccgcgctgt gctgctggat
    27661 attacctgtg actctgacgg tgctatcgac cactatatcg atggtgatgg tattgccacg
    27721 acaatgccta tgccggagta cgatccagag aatccgccga tgctcggttt ctttatggtc
    27781 ggcgcatatc aggagatcct cggcaacatg cacaacctgt tcggtgatac cgaagcggtt
    27841 gacgtgttcg tcttccctga cggtagtgta gaagtggaac tgtctgacga aggcgatacc
    27901 gtggcggaca tgctgcaata tgtacagctc gatccgaaaa cgctgttaac tcagttccgt
    27961 gatcaagtga agaaaaccga tcttgatgct gaactgcaac aacagttcct tgaagagttc
    28021 gaggcaggtt tgtacggtta tacctatctt gaagatgaat aattaagatt attctgctgg
    28081 cgggtcgatt tatttacgta aatcggtccg ctttcttttt atatattctt aaaaataatt
    28141 tagctgcttt tcttattaaa taatctccct acaatcgctg cattaattaa atgcagaggg
    28201 aacggttatg aaaaaatgga aagtacgcag cgcgttggtg gcattaatcg tgctgctggc
    28261 cggttgttcc agtaatgcgc agtataatag cagtgcatcg ggcaatgtcg gcaccgcctg
    28321 gggtggggat gtgcactcaa cggttcaggg cgtatctgct gagcgagcct ggcgcgatcc
    28381 ggcggagatg atcgttatta gttattcgac taatgttcct tccggctacg atcgcgttta
    28441 tagcattcgt attaacgaac tggaatatgc tattcgcgat ggtaatttta attctctacc
    28501 tattacgcgt gtttatgatt catctaataa tgaaccacgc tatattgtcc atgccagagt
    28561 cggaatgaat tatcaattat atgtccgtaa ttacagtcgt aacaccaact acgagatcgt
    28621 tgctacggtt gatggcctgg atgtgctgaa cggcaagcaa ggttcgctga ataacaacgg
    28681 ctatattgtg aatgccggag attcgttagc gattaaagga ttccgcaaag ataaacatac
    28741 cgaagcggct tttcagttcg ccaacgttgc tgattcctac gcagccaatt cggcgcaagg
    28801 cgatgtgcgt aataccggtg taatcggctt tgccgccttt gaattacaag gccaggcgca
    28861 aaacgcgctg ccaccgtgct ctggacaagc ctttccggca gataacaatg gctatgcgcc
    28921 gccaccgtgt cggaaatagc aacctcgctt gatgcctcgc ccatttaagg cgataattag
    28981 cacaaacttg tgtttcgcaa atccaaccct tcctcgtcgg gcctaacgac gcggaagggt
    29041 ttttttatat cgactttgta ataggagtcc atccatgagc accttaggtc atcaatacga
    29101 taactcactg gtttccaacg cctttggttt tttgcgcctg ccgatgaact tccagccgta
    29161 tgacagcgat gctgactggg tgattactgg tgtgccgttc gatatggcca cttctggtcg
    29221 tgcgggggga cgccacggtc cggcagctat ccgtcaggtt tcgacgaatc tggcctggga
    29281 acacaaccgc ttcccgtgga atttcgacat gcgtgagcgt ctgaacgtcg tggactgtgg
    29341 tgatctggta tatgctttcg gcgatgcccg tgagatgagc gagaagctgc aggcgcacgc
    29401 cgagaagctg ctggctgccg gtaagcgtat gctctccttc ggtggtgacc actttgttac
    29461 gctgccgctg ctgcgtgctc atgcgaagca tttcggcaaa atggcgctgg tacactttga
    29521 cgcccacacc gatacctacg cgaacggttg tgaatttgac cacggcacca tgttctatac
    29581 cgcgccgaaa gaaggtctga tcgacccgaa tcattccgtg cagattggta ttcgtaccga
    29641 atttgataaa gacaacggct ttaccgtgct ggacgcctgc caggtgaacg atcgcagcgt
    29701 ggatgacgtt atcgcccagg tgaaacaaat tgtgggtgat atgccggttt acctgacctt
    29761 tgatatcgac tgcctggatc ctgcatttgc gccgggaacc ggtacgccgg taattggcgg
    29821 tctgacctcc gatcgcgcta ttaaactggt acgcggcctg aaagatctca acatcgttgg
    29881 gatggacgta gtggaagtgg ctccggcata cgatcagtcg gaaatcaccg ctctggcagc
    29941 ggcaacgctg gcgctggaaa tgctgtatat tcaggcggcg aaaaagggcg agtaagcacc
    30001 agatgcgatg cgcacgggta aaacgtgcca ttaatgtcgg atgcggcgtg aacgccttat
    30061 ccgacctacg ttcggcaccc gtaggccgga taagatgcgc cagcatcgca tccggcaatg
    30121 cgcacaaggt aacaaatgtg ccattcatgt cagatgcggc gtgaacgcct tatctgacct
    30181 acgttcgaca ccaccaggct ttacttaatc ccatccgcgc tcatgcgatc gcgaatatgc
    30241 tgggcgcgtt cggcggatgc aggatggtcg tcaaacattg agctttggcg accttcttcc
    30301 agttttgcca gtttttcaaa gctggtggca agacctgccg ggctgatgcc gcgttggcgc
    30361 agaagatcgt aagaataatc atcggcttct gcttcctggc gctgggagaa ttgcgaatta
    30421 actaatttct cgcccagatc accgagttgt gattgagata aactgccgac aatcccgccc
    30481 gccgaggccg cagctactcg tacggcattt gtaccaagtg ccacctgcat tccttttttc
    30541 acatggccta atgccacgtg ccccatttcg tgaccgatca ccgcttcgac ttcgttatcc
    30601 gtcatcatat ccatcagccc gctatagaca cggatacagc cgttagccat tgcaaaggcg
    30661 ttcacatcct tcgccatata cactttgtaa tttaccggct gaccgttgat attgttgcct
    30721 agcgcattgg caatagttgt cagacgttta gcgtattcgc tattggctgg cgcaatcgtc
    30781 gccttgctgt ccatctcctg acatgcctga tcgctcaggg ttttcacctg cgcatcactc
    30841 aaactgtaag cctgaaaagc ttccgctcct gatgagagca gtccgttgga gtccatattc
    30901 tggcaaccgg tcagtaccgt tgccacgctc attgctacca ataaggcgcg aattttcatg
    30961 ttttttcttc cgcacaaggt caggattatt ctaaaaatgc gccgtttgca ggtgaaccga
    31021 cgctcagtct cagtataagg aaaagcgcag cggacgggcg agtagattgc gcaacatgcg
    31081 agcatgatcc agagatttct gaagcggcaa aaggatgttc catgtacatg acgcgcggct
    31141 tgcggtaaat tgttggcaaa ttttccggcg tagcccaaaa cgcgctgtcg tcaagtcgtt
    31201 aagggcgtgc ccttcatcat ccgatctgga gtcaaaatgt cctcacgtaa agagcttgcc
    31261 aatgctattc gtgcgctgag catggacgca gtacagaaag ccaaatccgg tcacccgggt
    31321 gcccctatgg gtatggctga cattgccgaa gtcctgtggc gtgatttcct gaaacataac
    31381 ccgcagaatc cgtcctgggc tgaccgtgac cgcttcgtgc tgtccaacgg ccacggctcc
    31441 atgctgatct acagcctgct gcacctcacc ggttacgatc tgccgatgga agaactgaaa
    31501 aacttccgtc agctgcactc taaaactccg ggccacccgg aagtgggtta caccgctggt
    31561 gtggaaacca ccaccggtcc gctggggcag ggtattgcca acgcagtagg tatggcgatt
    31621 gcagaaaaaa cgctggcggc gcagtttaac cgtccgggcc acgacattgt cgaccactac
    31681 acctacgcct tcatgggcga cggctgcatg atggaaggca tctcccacga agtttgctct
    31741 ctggcgggta cgctgaagct gggtaaactg attgcgttct acgatgacaa cggtatttct
    31801 atcgatggtc acgttgaagg ctggttcacc gacgacaccg caatgcgttt cgaagcttac
    31861 ggctggcacg ttattcgcga catcgacggt catgacgcag catccatcaa acgcgcagta
    31921 gaagaagcgc gcgcagtgac tgacaaaccg tccctgctga tgtgcaaaac catcatcggt
    31981 ttcggttccc cgaacaaagc cggtactcac gactcccacg gtgcgccgct gggtgatgca
    32041 gaaatcgctc tgacccgcga acagctgggc tggaaatacg caccgttcga aatcccgtct
    32101 gaaatctatg cgcagtggga tgcgaaagaa gcaggccagg cgaaagaatc tgcatggaat
    32161 gagaagtttg cggcttacgc gaaagcttat ccgcaggaag cggctgaatt tacccgccgt
    32221 atgaaaggcg aaatgccgtc tgacttcgac gccaaagcga aagagtttat cgctaaactg
    32281 caggctaatc cggcgaaaat cgccagccgt aaagcgtctc agaatgctat cgaagcgttc
    32341 ggcccgctgt tgccggaatt cctcggcggc tccgctgacc tggcaccgtc taacctgacc
    32401 ctgtggtctg gttctaaagc aatcaacgaa gatgctgcgg gtaactacat ccactacggt
    32461 gttcgcgagt tcggtatgac cgcgattgct aacggtatct ccctgcacgg tggcttcctg
    32521 ccgtacacct ccaccttcct gatgtttgtt gaatacgcac gtaacgccgt acgtatggct
    32581 gcgctgatga aacagcgtca ggttatggtt tacacccacg actccatcgg tctgggcgaa
    32641 gatggcccga ctcaccagcc ggttgagcag gttgcttctc tgcgcgtgac cccgaacatg
    32701 tctacatggc gtccgtgtga ccaggttgaa tccgcggtcg cgtggaaata cggtgttgag
    32761 cgtcaggacg gtccgaccgc gcttatcctc tcccgtcaga acctggcgca gcaggaacgt
    32821 actgaagagc aactggcaaa catcgcgcgc ggtggttatg tgctgaaaga ctgtgctggt
    32881 cagccggaac tgatcttcat cgcgaccggt tcggaagttg aactggctgt tgccgcctac
    32941 gaaaaactga ctgccgaagg cgtgaaggcg cgcgtggttt ccatgccgtc taccgatgca
    33001 ttcgacaagc aggatgcggc ttaccgtgaa tccgtactgc cgaaagcggt tactgcacgc
    33061 gttgcggtag aagcgggtat tgctgactac tggtacaagt atgttggcct gaatggcgct
    33121 atcatcggta tgaccacctt cggtgagtca gctccggcag agttgctgtt cgaagagttc
    33181 ggcttcaccg tcgacaacgt cgttgcgaaa gcaaaagaac tgctgtaact agcatttcgg
    33241 gtaaaaaggt cgcttcggcg acctttttta ttaccttgat atgtccgttt gcggacaagc
    33301 aatagataaa g
//
LOCUS       DAZFKY010000031        33307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFKY010000031 DAZFKY010000000
VERSION     DAZFKY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(62..1243)
                     /locus_tag="ACHKCL_001333"
     CDS             complement(62..1243)
                     /locus_tag="ACHKCL_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5762336.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            complement(1439..1533)
                     /locus_tag="ACHKCL_001334"
     tRNA            complement(1439..1533)
                     /locus_tag="ACHKCL_001334"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Sec,seq:tca)
     gene            1826..3208
                     /locus_tag="ACHKCL_001335"
     CDS             1826..3208
                     /locus_tag="ACHKCL_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5762337.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3218..5536
                     /gene="yicI"
                     /locus_tag="ACHKCL_001336"
     CDS             3218..5536
                     /gene="yicI"
                     /locus_tag="ACHKCL_001336"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5762338.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5589..7298)
                     /locus_tag="ACHKCL_001337"
     CDS             complement(5589..7298)
                     /locus_tag="ACHKCL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5762339.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHKCL_001338"
     CDS             complement(7419..8810)
                     /gene="xanP"
                     /locus_tag="ACHKCL_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5762340.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9090..10295
                     /gene="gltS"
                     /locus_tag="ACHKCL_001339"
     CDS             9090..10295
                     /gene="gltS"
                     /locus_tag="ACHKCL_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5762341.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10298..11164
                     /locus_tag="ACHKCL_001340"
     CDS             10298..11164
                     /locus_tag="ACHKCL_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762342.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHKCL_001341"
     CDS             complement(11149..13230)
                     /gene="recG"
                     /locus_tag="ACHKCL_001341"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5762343.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHKCL_001342"
     CDS             complement(13236..13925)
                     /gene="trmH"
                     /locus_tag="ACHKCL_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5762344.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHKCL_001343"
     CDS             complement(13932..16040)
                     /gene="spoT"
                     /locus_tag="ACHKCL_001343"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5762345.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHKCL_001344"
     CDS             complement(16059..16334)
                     /gene="rpoZ"
                     /locus_tag="ACHKCL_001344"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5762346.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHKCL_001345"
     CDS             complement(16389..17012)
                     /gene="gmk"
                     /locus_tag="ACHKCL_001345"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5762347.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17270..18952
                     /gene="ligB"
                     /locus_tag="ACHKCL_001346"
     CDS             17270..18952
                     /gene="ligB"
                     /locus_tag="ACHKCL_001346"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5762348.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18949..19566)
                     /locus_tag="ACHKCL_001347"
     CDS             complement(18949..19566)
                     /locus_tag="ACHKCL_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5762349.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHKCL_001348"
     CDS             complement(19858..20682)
                     /gene="dinD"
                     /locus_tag="ACHKCL_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO5762350.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20903..21766)
                     /locus_tag="ACHKCL_001349"
     CDS             complement(20903..21766)
                     /locus_tag="ACHKCL_001349"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5762351.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21893..22609
                     /gene="rph"
                     /locus_tag="ACHKCL_001350"
     CDS             21893..22609
                     /gene="rph"
                     /locus_tag="ACHKCL_001350"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5762352.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22675..23316
                     /gene="pyrE"
                     /locus_tag="ACHKCL_001351"
     CDS             22675..23316
                     /gene="pyrE"
                     /locus_tag="ACHKCL_001351"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5762353.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="ACHKCL_001352"
     CDS             complement(23353..23949)
                     /gene="slmA"
                     /locus_tag="ACHKCL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5762354.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24056..24514)
                     /gene="dut"
                     /locus_tag="ACHKCL_001353"
     CDS             complement(24056..24514)
                     /gene="dut"
                     /locus_tag="ACHKCL_001353"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5762355.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="ACHKCL_001354"
     CDS             complement(24492..25712)
                     /gene="coaBC"
                     /locus_tag="ACHKCL_001354"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5762356.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25884..26552
                     /gene="radC"
                     /locus_tag="ACHKCL_001355"
     CDS             25884..26552
                     /gene="radC"
                     /locus_tag="ACHKCL_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5762357.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26769..27005
                     /gene="rpmB"
                     /locus_tag="ACHKCL_001356"
     CDS             26769..27005
                     /gene="rpmB"
                     /locus_tag="ACHKCL_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5762358.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27026..27193
                     /gene="rpmG"
                     /locus_tag="ACHKCL_001357"
     CDS             27026..27193
                     /gene="rpmG"
                     /locus_tag="ACHKCL_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5762359.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27291..28100
                     /gene="mutM"
                     /locus_tag="ACHKCL_001358"
     CDS             27291..28100
                     /gene="mutM"
                     /locus_tag="ACHKCL_001358"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5762360.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="ACHKCL_001359"
     CDS             complement(28139..28618)
                     /gene="coaD"
                     /locus_tag="ACHKCL_001359"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5762361.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="ACHKCL_001360"
     CDS             complement(28626..29903)
                     /gene="waaA"
                     /locus_tag="ACHKCL_001360"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5762362.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30316..31374
                     /gene="rfaQ"
                     /locus_tag="ACHKCL_001361"
     CDS             30316..31374
                     /gene="rfaQ"
                     /locus_tag="ACHKCL_001361"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5762363.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31371..32495
                     /locus_tag="ACHKCL_001362"
     CDS             31371..32495
                     /locus_tag="ACHKCL_001362"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5762364.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32488..33285
                     /gene="rfaP"
                     /locus_tag="ACHKCL_001363"
     CDS             32488..33285
                     /gene="rfaP"
                     /locus_tag="ACHKCL_001363"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5762365.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7857 a         8285 c         8884 g         8281 t
ORIGIN      
        1 ccatccgcct gttggggctg gatggttatt cattattttt gtgtcaccga actttcattc
       61 atcaggccag ctcagaaagt gaatcaatgt agtcagcgta ccattgcatc atttcacggc
      121 gaccatcgag atactgggcg tggttatagg tacccctgat ggagttttta tccacatgag
      181 ccaactgcat ttcaatccag gcagaattaa agccttgttc gtgcagaatt gtgctcatgg
      241 tatgcctgaa accgtgccct gtgagtcttc ccttataccc cagcaattca atgacctggt
      301 taatgctctc cttactaatt ggcttgcgag gatcgttcct accgataaag accagaggat
      361 gatgttttga gattggttca agttgcttaa atagcgcaat aacctgctca gagggcggca
      421 caatatgagg ccgtttcatc ttcattactt ctgccgggat ctcccataat tttgcctcaa
      481 aatcaatatc ctcccaatgc gcgaaacgca actcttgcgt tcgtacaccg gttaacataa
      541 tgatctgagt agctgtctta gtgatgatac ttccggtata acccgccaga tcagtaagga
      601 agtggggaag ttcattggca gtaagaaagg gaaaatgtac ttttttcggt gtagctaaag
      661 cactggcaag atcgggagca gggttatagt cggctcgacc agtaacaatt gcatagcgaa
      721 acacctcacc acaacgttgg cggaccttgc gcattttctc cagtgctcca cgtttctcca
      781 tcttacgtag tgcttccaat agctccatcg gcttgatttc ggcaataggt cgcttgccga
      841 tgtaggggaa gatatctttc tcaaaggtgt cgattatttc atcgcggtag cgcagtgacc
      901 agcgatcggc tttcgtctga tgccactcgc gagcaacggc ctcgaaagtg tttttcaaac
      961 tcatttgcag cgctattttt tcttcttttt ttgcctcacc cggatcgcca ccagccgcaa
     1021 gtattttcct ggcttcactg cgtttctcac gtgcgtcggc aagcgttaca tcggggtata
     1081 cgccaataga cagcatcttt tctttaccgg cgaagcggta cttcatacgc cagtatttgg
     1141 aaccattagc gttaatcagc agatacatac ctccgccatc agccagctta tagggccttt
     1201 cttttggctt cgcggttttc actttaacat cggttagcgc catgacatcc tccagctttt
     1261 gatgaatcca tcctcctggg ggaatttccg ccagtaacca acctgatacc cccaatgcac
     1321 ccccacaaac aacttgatat gggttaaagt cgtttgacct caggatacaa agaaaacctg
     1381 atatataagg gattataagg ggctttgttg atgtcgggag agttcaggag aggcaaactg
     1441 gcggaagatc acaggagtcg aacctgcccg ggaccgctgg cggccccaac tggatttgaa
     1501 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
     1561 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
     1621 acacgcagcg tattatcacg aaagcattat aattcatata aaactcatat tttacaatga
     1681 tatatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
     1741 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
     1801 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
     1861 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
     1921 gatgttcttt tataccgata attttggcat acctgccgga tttgtcggaa ccatgttttt
     1981 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
     2041 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgtgt ggcgcactgc catttgggat
     2101 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     2161 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     2221 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     2281 tgtgctggcg accgcgggag gcatgctctc tactgttctg atgatgccac tggttaattt
     2341 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     2401 ggcattcatg atgctggcat tctgtttctt caccactaaa gaacgcgttg aagcaccacc
     2461 tacaacgacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     2521 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtccgcggtg gagcgatgat
     2581 gtattacgtc acatggattt tgggtacgcc ggaagtgttt gtcgcttttc tcaccactta
     2641 ttgcgtgggt aacctgattg gttccgcact ggcaaaaccg ctgaccgact ggaaatgtaa
     2701 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     2761 tgttcctatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtattgca
     2821 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     2881 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     2941 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     3001 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     3061 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     3121 gcacaatctg aaaaccgtta tggaacagct ggcccagggc aaacgccgtt gccagcaaca
     3181 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg acggaaactg
     3241 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     3301 gggtaatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgaa cctggcagct
     3361 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtacg
     3421 gattgagcat tttcaggggg cgctgaataa cggtcctcat tatccgctca atattttgca
     3481 agacgtgaag gtcacaatcg aaaacacaga acgttacgct gagtttaaaa gtggcaactt
     3541 aagcgcgcgt gtcagcaaag gtgagttctg gtcactggat tttctgcgca acggcgaacg
     3601 tattaccggt agtcaggtga aaaataatgg ctacgtgcag gacacgaata atcaacgaaa
     3661 ttatatgttt gagcggctgg atcttggcgt tggcgaaaca gtttacggtc tgggagagcg
     3721 ctttactgcc ctggtgcgca atggccagac ggtagagacc tggaaccggg acggcggcac
     3781 aagtactgaa caggcgtata aaaatatccc gttctatatg actaaccgtg gctatggggt
     3841 actggtcaat catcctcaat gcgtctcttt tgaagtggga tcggagaaag tctccaaagt
     3901 gcagttcagc gttgagagtg aatatctcga atactttgtt attgacggcc cgacgccgaa
     3961 agcggtactt gatcgttata cccgttttac tggtcgtccg gcgctgccgc ccgcgtggtc
     4021 cttcggcctg tggctaacca cttcatttac caccaactac gacgaagcga cggtaaacag
     4081 ctttatcgat ggtatggcgg gacgcaatct gccactgcat gtttttcact ttgactgttt
     4141 ctggatgaaa gccttccagt ggtgcgattt tgagtgggac ccgctgactt tcccggaccc
     4201 ggaagggatg atccgccgcc tgaaagcgaa agggctaaaa atctgcgtct ggattaaccc
     4261 ctatatcggg caaaaatccc ctgtctttaa agagttacaa gagaaaggct atttactcaa
     4321 acgcccggac ggttcgttat ggcagtggga taaatggcag ccaggtctgg cgatttatga
     4381 ctttaccaat ccggatgcct gcaaatggta cgccgacaaa ctgaaaggtc tggtcgcgat
     4441 gggcgttgat tgctttaaga ccgactttgg cgaacgtatt ccaatcgatg ttcagtggtt
     4501 tgacggttcc gatccgcaga aaatgcataa ccattatgcg tacatctaca acgaactggt
     4561 gtggaacgtg ctcaaggaca ccgttggcga ggaagaagcc gtcttgtttg ctcgctcggc
     4621 ctccgttggt gcgcagaaat tcccggtaca ctggggtggt gactgttacg ctaactacga
     4681 atcaatggcg gaaagcctgc gcggtggttt gtctattggc ctttcaggtt ttggtttctg
     4741 gagccacgat atcggcggct ttgagaatac cgctctggcg cacgtttaca aacgctggtg
     4801 cgcgtttggt ttgctctcca gccatagccg tttacatggc agcaaatctt atcgtgtgcc
     4861 gtgggcctac gatgatgagt cctgtgatgt ggtgcgcttc ttcacgcaac tgaaatgccg
     4921 catgatgccg tatctgtatc gtgaagctgc tcgtgcgaac gcgcggggta cgccgatgat
     4981 gcgggccatg atgatggagt tcccggacga tccggcttgt gattaccttg accgtcaata
     5041 catgttaggc gacaacgtga tggttgctcc ggtgttcagt gaagcgggcg atgtgcagtt
     5101 ctacttgccg gaaggtcgct ggacacatct gtggcacaac gatgaactcg atggtagtcg
     5161 ctggcataaa cagcagcaca gcttcctgag tctgcccgtt tatgtgcgtg ataacaccct
     5221 actggcgctg ggcaacaacg agcaacgtcc cgattacgcg tggcacgaag gcacggcatt
     5281 ccagctcttt aatctacaag acgggcatga agccatctgt gaagtgcccg ctgctgacgg
     5341 ttccgttctt ttcaccctga aagcggcgcg tactggcaac acgattactg tgactggtgc
     5401 gggcgaggcg aagaactgga cactgtgctt gcgcaatatt gtgaaagtaa atggtctgca
     5461 aggtggttcg caggctgaaa gtgagcaggg gctggtggtg acgcctcaag ggaatgcgct
     5521 gacaattacg ttgtaatcta ttgatggaat gccgggtacg tctgagtcgt acccggcgag
     5581 attgattttt actgcaccgg ctctgtcgat gtaacttcgc tactggaaac gacgcccgca
     5641 ttcatggttt gagtcacttg ctgctttgcg gcgttcacgg catggagttg cccgctaacc
     5701 gtaggtttca gcgggacatt ggcctgaata tcgccactgg cggtaagctg aatattgccg
     5761 tcgccagtca acggtaattc aggccatccc cattgttgca aaatattcac cggcacaccg
     5821 cgtccattca ggctgatatg ggtctgacgt tgtggcgttt gtgaaacact ggcagtggct
     5881 tccagaatgc ctttttcagt aaatgcactc agttcgctga tattcaccgt gctgctgttg
     5941 gcggtcagcg ccagcgacgg gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca
     6001 ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag cgtcaggttg
     6061 gcaccgtaac catcgagcgt ggtgagctgc cacgggaagt cagggtcgat atcaatgatc
     6121 agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt
     6181 tccatccaca actgttgcca gtttttcggc aaggtatatt ccagcccggc aattgccgca
     6241 tcatcaagga tcaacgtttt cccgtcacgc agccagttcc ctgacgttct gaccataccc
     6301 ccttcccagc ggctggtgaa ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt
     6361 ataatcgggt caaataaatg cagcgaacca taaatgaact cgctagcgtt catcgacagt
     6421 ttgccttctt gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg
     6481 aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatcacttc caggcgacca
     6541 atctgcaacg acgggacaga gcgtaatggc gcaaagaagt cggttagcga tttttcgctt
     6601 tgtagacgga tatccgccat gcgcagattt tccacttgcc agctgccgtc ggcgttacgc
     6661 tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg
     6721 cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc attgagcgaa
     6781 agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc ggcttctggt
     6841 gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc gctattcggg
     6901 ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag cggcgcggtc
     6961 tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg gcgtggttcg
     7021 gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt cgcgggctga
     7081 ccatcacgac caaacgtgac gttctccagc acgatatgag atggcgcgga aaaacggtga
     7141 tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc gctgatatgt
     7201 tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac taacagagcg
     7261 atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc cgtgaaatgc
     7321 acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt
     7381 gagactgcgg cggtaattat cttaccgccg ccagtgaatt actgtttttc tggtgggaaa
     7441 atcagattca gcacgatggc agtaataccg cccgcggcga tcccggagga gagcaggttt
     7501 ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc cagaccaacc
     7561 gccagcgaca gcgcgataat cagaatcgcc cgacggttca gcggctcacg agaaacgata
     7621 cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt
     7681 tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac gatcaacatc
     7741 agcgcgacga caaaaccgac atagcggctg gcaacaccag tcaactggat cactccgttg
     7801 ttttgcccga agcaggagtt cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg
     7861 ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga cactggctgt
     7921 tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt gatcataaag
     7981 accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata gagcggcgtt
     8041 ggcaccataa tcagttcttg cgtcatcggt tcgttgcttt ctggcaacat gcccataaac
     8101 cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg taagtaaggg
     8161 ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag cagcagattt
     8221 ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat ggacgttaac
     8281 ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaac cagcggcgta
     8341 ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtgca acttgccagc
     8401 atcaacgtgc cgaacaaagc cgccatcatg gtaggaacat cagcaccacc ggttttcagc
     8461 gcggtaccgc ccataatcag cggggcaaca aagttgaagc tggtgccctg aatagacaac
     8521 agcccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac accggaggca
     8581 aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag
     8641 attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcagatgctg acaggcggca
     8701 aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc gctgttttga
     8761 gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat cgaaaacaat
     8821 cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac gtttgccaca
     8881 tcctcatcta aatatttaaa tgagataaat gcactttttt atataacttt tggtattttt
     8941 ctgcttaaaa tccatgccat ttgttcatta acagctgaga atttgaccca ggaaagcata
     9001 actcgatacc cgtcatagtt catgaagttg ccactgcgcg atgaagtatg acgagtatga
     9061 aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta gcaacgcttg
     9121 ttgccgcaac gctgacgttg ttgctcgggc gtaagttggt ccattccgtc tcctttttga
     9181 agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg gcgctactag
     9241 tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc gatccgttaa
     9301 tgctggcttt cttcgccacc attggcctga acgccaacat tgccagtttg cgtgccggtg
     9361 ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg caaaatgcca
     9421 ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg ttggccggtt
     9481 ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg ttcattgaac
     9541 gttatggctt caccaatgcg acggaagtgg cgatggcctg tgcaacgttc ggtctggtgc
     9601 tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc accacgccga
     9661 acggtattcc ggatgaccag gaagtcccga cggcgtttga aaagccggat gtgggccgca
     9721 tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc ctgacggtgg
     9781 ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc ttcgtctgtg
     9841 tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt taccgcgtct
     9901 ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg gcgatggcgc
     9961 tgatggggct gaaactgtgg gagctggctt cgctggcgct gccgatgctg gcgattctgg
    10021 tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc atgatgggca
    10081 aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc ggtgcaacgc
    10141 caacggcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg cacatggcgt
    10201 ttttggtggt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg ctggtgatta
    10261 agctgtattt aatgttgccg atttttgccg gttaaaaatg aaaaagcgtc ggaaggaacc
    10321 ggaaacgcta cgtgaacact gtcgccatat ttttggtgat gagccgccag tgctctgtgt
    10381 atgggaaacg gaatttgatt acgcagatgc cgaactcaag gcgctggcgg ctaaagagtg
    10441 gcagcagatc agcgaatggg atttgagtgc ttactatgta ctcaatctcg tttataacga
    10501 accgatgcaa atagagttgt ttcgctatct gtttccgctc tgcctggcgc aatggcatga
    10561 aaccgtttta gctggcggtt atggtgatca cttcgaagaa tccttaatga aggcattgtg
    10621 tcggccctat ctctggcagg agatgatgaa tgcttcccaa cgccagcagg tacggcaatt
    10681 tttactggac accgcgttgc agcgtatgga taacgagcgt ggtttcaata acgttctttg
    10741 ctggctggcg gtgttcaata ctctgggtgg ggccgcgccg ttaattcgtt ctctctggtc
    10801 gcgctggtgg gcgctggata cgccaggaaa agcggtgtgt gctattcagt atgccgcgca
    10861 tctaatttat cccatagaag ccaatccgtt atggtcgcaa gaatggatag gctgggggca
    10921 tccattaggc cataaagatg gctggtcctc tgataatcgc gcttttcttc gccaaatgtt
    10981 aacacctgaa atgattgtgg ctggcgtaca ggcagcggct gaaattctgc gtggtgaacc
    11041 ggaaggcgca atggcggcgc gaattgcaca ggatgcgtat gaggcaatgg atatcctcac
    11101 tatccagatt gaagatttac tgcgtgactt gtcttgtgat gagtctggtc atgcgttgga
    11161 gtaacgttcc gtctccggca tccagcgttc tatcagggct tttgcttgtt gtgggtaacg
    11221 ttcgtgaata tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa
    11281 atccgccact ttaaactcag cattacccgt ctgacgcgta cctaacagtt cacccggtcc
    11341 gcgaatctct aagtctttct gtgcaatcac aaaaccgtcg ttgctgtcgc gcagcacttg
    11401 cagccgaatt tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaagc
    11461 caccgcacca cgacctacac gcccgcgaag ctggtgtaac tgcgccagac ccagacgctc
    11521 agggttttcg ataatcatca ggctggcgtt aggcacatca acaccgactt caataacggt
    11581 agtggcaact agcagatgta gctcaccttg tttaaacgac gccatcaccg cctgtttctc
    11641 ggctggtttc atccgcccgt gtaccaggcc aacattcaat tctggtagcg ccagtttcaa
    11701 ctcttcccag gtagcttccg ccgcctgcgc ttccagtaac tctgactctt caatcaacgt
    11761 acaaacccag tacgcctgac gaccttcagt tatgcaggcg tggcgcacgc ggtcaatgat
    11821 gtcggtacgg cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggcggcag
    11881 ctcatctatc accgaggtat cgagatcggc atacgccgtc attgccagcg tgcgggggat
    11941 cggcgtggcg gtcatgatca actgatgcgg atggaagcct tgctgctggc ctttctccca
    12001 taacgccaga cgctgatgca cgccaaagcg atgttgttcg tcgataatca ccagcgccag
    12061 accgttaaac tgcacctgtt cctggaagat ggcgtgcgta ccaacaatca tctgcacctg
    12121 accgctggcg atggcttcct gctgagacag tcgcgctttg cctttctgct taccggcgag
    12181 ccagcccact tcgataccga gcggttcaaa ccagttgcgg aagttattgg cgtgctgttc
    12241 ggcgagtaat tcggttggtg ccatcaatgc cacctgtttg ccgtgggcaa tcgcacgcag
    12301 cgcggcgagg gcggcgacca gcgttttacc ggaacctaca tcgccctgta ccaggcgcat
    12361 catcggcaca tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc
    12421 cgttggcttg aacggtaagg ctgcgaggag tttatttttc agcgcatcat tggcgctcag
    12481 cggctgggca tgaaaacgct gtgctccggc acgtaaggct aacatgctga ggttgtgcgc
    12541 cagcagttct tccagaatca gacgacgttg cgctggatgc tgcccggttt ccagatcgct
    12601 aagctgtagc gtcggtggcg ggcggtgcaa agtgcgcaac gcttccggta gcgtcatcat
    12661 tccttgtgac agttccggcg gcaggagttc ttcaatggcg caggtgtcga gcagatccag
    12721 cgcctggtcg gttaatttac gcagcgtggc ctgctttacg ccttccgttg ttggataaac
    12781 cggcgtgagc gtttcctgta attctggcgt gctgagatcg ccctgcacgc ggtattccgg
    12841 gtggatcatc tccgcaccat atttaccgcg ctttgcttcg ccataagcca gtacacggcg
    12901 gcccgtcgcc aggctatttt tcattgccgc gctgaaattg aaaaagcgca tggtgaggat
    12961 gccggaaccg tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca
    13021 gttcagcact tcgccttcca ccgtggcata aacgcccggc agtagttctc cgatggggta
    13081 gagatgggtg cgatcttcgt agcgcagggg aaggtgtaag agtagatcct gcacggtatg
    13141 caggttgatt ttcgccagtt tgttactaag tgctgcgcca acgcccgtta gggaactgag
    13201 tgggacagca tctaacaggc gacctgtcat ggcacttacc ctgcagcctg catagtagcc
    13261 caccagtcgg catcagcttc gatctcgcct tgctgattga cgtggggata aggcaggcct
    13321 ttgcgttttg cgactttcgc cagcaccgga tagccgcctt caaacaacag gcgttgttgc
    13381 tctgcttccg gcaacatgct gttttcacgc aggtacatgc ctgcattttg ccgctgacgc
    13441 tgggcttcgt aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg
    13501 atcatcggaa tgatgatgtc ctgatccgcc agggccaatg cttcctgcgt gatgcccgtt
    13561 ttctcctgtc ccatcaaaat gcaggtcggg cgagtgtaat caatttcgcg gaaatcgaca
    13621 gcgttatcag aaagatgggt tgccagaatc tgcatgccct ggcctttgag atgagcgacg
    13681 gcatcgccaa tggtgcggtg tgttttcacc tgtacccagc tgttactacc cgccgctgcc
    13741 gaagccatgg tgcgcatgcg gctaccaggc cagacggcgt gaacttcatg tacgccaacg
    13801 gcatctgcgg tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag
    13861 acggtcagat caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg
    13921 ttcataaaac attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt
    13981 ttgcgcatga tattcgccag atgcacacgg tcacgagcgg tcagacgaat aaaggcgctg
    14041 tagacgcgac catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta
    14101 attgccgccg tcaggtttgc cagcgcaccc tgatgattga acatctccac cttgatttcg
    14161 gtgatgaact cctgcgccgt ctctttatcc cattccacag ccataaactt ctctggctct
    14221 ttctggtagc cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg
    14281 ctgacgtggg cgataatcgg gtcgccagga atggggcggc agcatttcgc aaaggtgatc
    14341 agcacgccat cagcgccttt gatcggcaga tgcccgtggc tttgagtcgc cggtggaatg
    14401 gaggcgtccc catgttgtag atttttcgcg accaccacgc tcattgcgtt accaaggccg
    14461 atttctgcca gcagatcgtc aagcgttgcc agcttcatgc gatccagctc gcgctgaata
    14521 ttttcctgcg ggatttcatt gagcttacgg ctaccaccca aagcatggtt gagcagacga
    14581 cggcccaggc ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg
    14641 cgcgctttcg agctaacgac aaagttcagc caagcggcat tcgggcgagc gcccggagcg
    14701 gtaatgattt caacggtctg accgctagta agcggctgcg acagcgggta aggctggcgg
    14761 tcgacgcgtg cgcccacgca ggcatggccg atatcggtat gcactgcata agcgaagtcg
    14821 acgggcgttg caccggcagg cagctcgaca atgcgccctt ccggtgtgaa aacgtaaatc
    14881 tcatccggga agagatcgga tttaacgctc tcgataaatt caaacgaact accggcgctc
    14941 tgttgcagct ccagcaggct ttgcatccag cgctgggcgc ggatttgtgc ggtagtactg
    15001 gtttcgccgt gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc
    15061 atatcttcgg tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg
    15121 tgcaacgact gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga
    15181 cgcggcttgt acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg
    15241 acgatcacgc ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc
    15301 attttacagt aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc
    15361 gcttcctgca aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg
    15421 ccgcgcgcgg ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca
    15481 aaacccagct cttcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcggg
    15541 ctgtaaattt cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc
    15601 agcgtgcgca tgttgtgggt acggtcggca agtttgatga ggatgacgcg gatatcctgc
    15661 accatcgcca taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac
    15721 ttgagtttat caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc
    15781 tgttccatat cctggtaggt ggcgggagta tcttcaatca cgtcatgcag cagcgccgcc
    15841 atcagcgttt catagtcgag tttcatctcg gccagaatgc aggcaaccgc taccgggtgc
    15901 gtgatatagg gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga
    15961 tacgcctgcc gcagacgctt gatttggtct tccggcaggt aggtttgaat cagttgattc
    16021 aggctttcaa acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata
    16081 gcggtaacgg cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg
    16141 tcgaggatct ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt
    16201 ttatcgtttt cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga
    16261 cgcgcggcga ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca
    16321 gttacgcgtg ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga
    16381 aatcaggttc agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg
    16441 ctcatgcgca gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag
    16501 tcatcattca caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc
    16561 gccatacgct ttgcaatgac ctcttcgctg tcctgatcgc gaccgcgtag acggcggtcc
    16621 agttcaattt tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga
    16681 atttgctgcg cgccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact
    16741 tgctcaatgg cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg
    16801 aacgcatctc tgctaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca
    16861 ccgtggactt caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca
    16921 tacaacggtt gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg
    16981 gcagaaacaa tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa
    17041 taaagcctac atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac
    17101 actttttgcg caaagttgtt gcgttttgcg tagctgtttc cgcatttgcc tgtgatttac
    17161 tgcaataaag acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt
    17221 gccttcacca cgttgtatat ctgccagcct gactacagcg gaggtgaaga tgaaagtatg
    17281 gatggcgata ttaataagta tcttgtgctg gcaatcatct gtgtgggcgg tctgtccggc
    17341 ctggtcgcca gccagagcac aggaagaaat tttccgcctg caacagcaaa taaaacagtg
    17401 ggacgatgac tactggaagg aaggaaaaag tgaggtggaa gacggtgttt acgatcagtt
    17461 aagcgcccgt cttacgcagt ggcaacgctg ctttgtgagc gagccccgcg acgtgatgat
    17521 gccgccttta aatggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc
    17581 ggataaaaac gcattaagtc tgtggatgcg agaacgtagc gatctttggg tgcagccaaa
    17641 agttgatggc gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag
    17701 tcgcggtaat ggcctgaaag gcgaggactg gacgcagaaa gttagcttaa tttccgctgt
    17761 gccgcaaacc gttagcgggc ctttagccaa cagtacgctt cagggggaaa tatttctcca
    17821 gcgcgagggg catatccaac aacaaatggg gggaataaat gcccgcgcaa aagttgctgg
    17881 cctgatgatg cgccaggacg atagcgacac gctgaattct ctgggcgttt ttgtctgggc
    17941 atggccggat ggaccgcagt taatgaccga tcgtttaaag gagctggcta ccgcaggttt
    18001 tactctaacg cagaggtata cccgtgcggt taaaaatgct gatgaggttg cgcgcgtacg
    18061 caatgagtgg tggaaagcga aattaccctt cgtcaccgat ggcgtggttg tgcgaggggc
    18121 gaaagaaccc gaatcccgcc attggctacc aggccaggca gaatggctgg tggcctggaa
    18181 atatcaacct gtagctcagg ttgccaaagt gaaggcgatt cagtttgcgg tgggtaagag
    18241 cggtaaaata tcggtggttg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca
    18301 gcgggtgaat attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca
    18361 gatcctcgtc agccttgccg gtcagggtat tcctcgcatt gatgatgtgg tctggcgcgg
    18421 tgcagaacgt acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt
    18481 tgcttctgat gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca
    18541 ggttctgggg ctggatggca ttggtgaggc cggctggcgc gcgctgcatc agactcatcg
    18601 ctttgagcat atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg
    18661 gatcgcgaaa agtaaaagtg cgcagctatg gcatcggttt aatctggctc gtaagcagcc
    18721 ttttactcgc tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag
    18781 tgatgaacgg tcctggtcgc aacttttatt tagcacggag cagttctggc agcagctgcc
    18841 ggggactgga tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa
    18901 gaagctgggc agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgtccttc
    18961 gtggctgtag taaaagaccg gtaatcccag cttaaggcgt agcgccagta agcgggcaaa
    19021 aaagccgaat accagggtgg agataattac cacatcatgg ttagaaacat agtgttgcag
    19081 cgcgatgtac agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac
    19141 cagtgggata cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac
    19201 cgccgcgaca acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat
    19261 gatagaaaag accaccagtc caagcgcatc gagcaccaga aacactttgc gcaggtaagg
    19321 cattacagga gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc
    19381 cgggtgtttg acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc
    19441 gccaattgcg gtggcggtcg caataataat tacgccaaat gtatccatgc ggcgtcgccc
    19501 ggccgccaga gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa
    19561 cagcatggtt ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac
    19621 gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt
    19681 ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata
    19741 ggttaaaatt gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc
    19801 tggactttgg cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta
    19861 tttcttctct gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg
    19921 caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgctacccac
    19981 atcaaagtgt gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc
    20041 ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag
    20101 gatcttctga ctctttttca gccccttcag ttggtggatg gctttctgat ctaatccacc
    20161 atacagcccc tggtaaccat gattctgaaa tatggcgaaa tcagtagctg tcgctaccgc
    20221 agcttgctgt gctgcctcaa ccaattgttt gttatgttct ttcaactcat ttctcagaaa
    20281 cagacgcttt tcgtcttcac gaagttgctt aaatgcttca tcgtcagcaa gctcctgccg
    20341 tcgggtctgg atagcaaaat aagtctgccc tgccgcaatg accggtttcg cagggtcgcc
    20401 gttttgtact accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg
    20461 agcccctgaa cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg
    20521 attgctggct tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc
    20581 ccgataatcc agtaacggtg ccaattctct agctgaccaa aattcttgcc cttcagtgcc
    20641 ataatgccgt atttcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata
    20701 tcgattgcaa agaaagtaaa tctgtaactg tatttatata cagttgtgag tatctcacgt
    20761 tctcagtgtg ctgggcaaaa aaacatcaca aaatctggag caggcacgca aaacagaatt
    20821 gaagttgcgg ggaagaaaaa tgccgctctg tttgagtggg cggcatttgt gaaggctgaa
    20881 aagtaagaat tggcgttacg agttattcaa tgttctgaat ctgctcgcgc atctgctcaa
    20941 tcaacacttt cagctcaatc gcggagtttg tcacttcggc attgatagac ttcgacgcaa
    21001 gagtgttcga ctcgcggttg aattcctgca tcataaagtc cagacgacga ccaacggctt
    21061 cttttttctt cagaatgttg taggtctctt tgacatgcgc ttccaggcgg tccagttctt
    21121 cggcaacgtc aattcgttgt gccagcagaa ccagctcctg ctccagacgg ttgttttcca
    21181 actgaacctg agcatcttcc agcttcgcga ccagacgctc acgctgccat tgcaggattt
    21241 ccggcatatg ggcgcggact ttgaccactt cggcggtgac gccttccaga cgctgctcga
    21301 tcaatgcttt cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac
    21361 catccagcgc cgcgagaatt tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca
    21421 tcacgcccgg ccagcgtaga atatcaaccg ggttgatttc cccttcgtca ctctgcattt
    21481 ttacccagtt cgcggcagtt accagctgtt tagccagttt ttcgttgagg atcagctcac
    21541 cttgcgcgct aacatctggc tcatagcgca gggtacattc cactttaccg cgcgtcaggc
    21601 gagaacgaat acgctcgcga acgacaggtt caaggctacg gaactgctcc ggcagacgaa
    21661 agtaagtttc cagataacgc tggtttaccg agcgcatttc ccaggttgcg ctcccccatt
    21721 cacccttgat ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac gttcctgttt
    21781 ataaaaggag aggtggaagg attatagcca tcgatgcctt gtcaggatag gaataaccgc
    21841 cggaagtccg tataatgcgc agccacattt gtttcaagcc ggagatttca atatgcgtcc
    21901 agcaggccgt agcaataatc aggtgcgtcc cgttaccctg actcgtaact atacaaaaca
    21961 tgcagaaggc tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat
    22021 tgaagaaggc gtgccgcgct tcctgaaagg tcagggccag ggctggatca ccgcagagta
    22081 cggcatgctg ccacgttcta cccacacccg taacgctcgt gaagcggcga aaggtaagca
    22141 gggtggacgc acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga
    22201 tttgaaagcg ctgggtgagt tcaccattac gctggactgc gacgtgcttc aggctgatgg
    22261 tggcacgcgt accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa
    22321 gctggtggaa aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc
    22381 tgtcggaatt gtgaacggcg aagcggtttg cgatctggaa tacgttgaag actctgccgc
    22441 agagaccgac atgaacgtag tgatgaccga agacgggcgc atcattgaag tgcaggggac
    22501 ggcagaaggc gagccgttca cccatgaaga gctactcacc ttgttggctc tggcccgagg
    22561 gggaatcgaa tccattgtag cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg
    22621 actgatgagt cgcctttttt ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa
    22681 ccatatcagc gccagtttat tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag
    22741 tttacgttga agtccgggcg caaaagcccc tatttcttca acgccgggct gtttaatacc
    22801 gggcgcgatc tggcactgtt aggccgtttt tacgctgaag cgttggtgga ttccggcctt
    22861 gagttcgatc tgctgtttgg ccctgcttac aaagggatcc cgattgccac cactaccgcc
    22921 gtggcgctgg cggagcatca tgaccttgac ctgccgtact gctttaaccg caaagaggca
    22981 aaagaccacg gtgaaggcgg caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg
    23041 gtagatgatg tgatcaccgc cggaacggcg attcgcgaat cgatggagat tattcaggct
    23101 aatggcgcga cgcttgctgg cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc
    23161 gagatttcgg cgattcagga agttgagcgt gattacaact gcaaagtgat ctctatcatc
    23221 accctgaaag acctgattgc ttacctggaa gagaagccgg aaatggcgga acatctggcg
    23281 gcggttaagg cctatcgcga agagtttggc gtttaaagaa actcgccgga tgaaaagtca
    23341 tccggcgtca tattactgca actgtgccgc aattagcggc cagcgggcgt caaaatcatc
    23401 cgtcgggcgg tatttaaatt cgctgcggac aaaacgtgac agcatacctt cacagaaggc
    23461 taggatctgg cttgccagca gggtttcatc ggtggtgtaa ccttcaccct cacgcattct
    23521 cttttcacgc aatacctgac gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg
    23581 cccttgcagg cgatcctgtt caaacattag cgcatgacca gtgaggatgc gggtcaggcc
    23641 aggattacgc tcaccaaaac cgagaagcag caacacaatc agacgcaggc gcgctgtggt
    23701 gtctttctca tctttcagaa tcagattgat gcgagtaatc aggctatctt cgataaactc
    23761 aatcaggcta tcgaacatgc gggtcttact ggggaagtgg cgatacagtg ccgcttcgga
    23821 aacgccgaca gaggcggcca gttttgccgt cgtgatacgt tggcttccat cgctggattc
    23881 cagcatcagc gccagagact gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt
    23941 ttctgccatg ttacaaaata cccctgaaaa taagcacttg ccaggcggca cccacgctat
    24001 gaccgcaaac gaaatgttta cggctatgtt atgacgttat tcggatgcgt atgtgttact
    24061 gacgaccaga gtgaccaaag ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca
    24121 gattaaattc agcctgtact accggaacaa aaatcatctg ggcgatacgt tcgccaggtt
    24181 gaatggtgaa gctgtcctga ccacggttcc acacggaaat catcaactgg ccctgatagt
    24241 cggaatcgat caatcctacc aggttaccaa gcacgatacc gtgcttatgt cccaatccgg
    24301 agcgcggcag catcattgcc gccagtgaag gatcggcaat atgaatcgcc agcccggtcg
    24361 gaaccagcgt agtgtcaccc ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt
    24421 caagtccggc agagccagag gtggcataag tcgggagcgg aaattccttc ccaacgcgcg
    24481 ggtccagaat cttaacgtcg atttttttca tcataacggg tcacgatctc gtcgagtaat
    24541 aattggccaa ggagctcttt gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa
    24601 aggtgtaatg cgttgttgtc gctgttaaat ccttgagttg gctgggaaac atcgttcgcg
    24661 cagatcagat caaggttttt acggatacgt ttttgccggg cgtattcttc cacattattt
    24721 gtttcggcgg caaatccaac gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg
    24781 acgatatcgg ggtttttaac catttttatt gttaattcat caccctgcgt ggcctgcttt
    24841 ttgattttct ctggggccac ggtagctgcg cgataatccg ccacggcggc gcagccgata
    24901 aaaatatttt gctgctgtac agaagcattc acggcggctt ccatttccag cgcggtcatc
    24961 acatcaacac gtttaacaaa cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg
    25021 acgttcgcgc cacgacgggc agcggcggcg gcgatagcaa aacccatctt gccggagctg
    25081 tgattagaga tataacgcac gggatcgagc ggttcacgcg tcgggccggc ggtaatcata
    25141 atgttcagat gtttcaggtc gttgacgggc gaaaaatgcg ctaccgccat atccacaatg
    25201 gttaacggat cgagcattcg cccaggaccg atatcaccac aagcctgact gccactgtct
    25261 ggcccccaga tgagcaaacc acgggaagca agcacctcta aattatgctg cgtggcagcg
    25321 gcacggtaca tctgctggtt catggcgggg agcacggcta caggcgcagg tgtagccaga
    25381 caaatcgtcg ataccaggtc attcgccatt ccggcagcaa cacgggcaat caaatctgcc
    25441 gtggcagggg cgagaatcac taaatcagcc catttaccca gctcaatatg gcccatagcg
    25501 gcttcggctg ccgggtccag caaactgtcg gaaacgggat aaccagaaac cgcctgcaag
    25561 ctaagtgggg tgataaaggc ttttgccgct tcggtcatgg ctacgcggac gtcggccccg
    25621 cgatcgcgca aacgacgcac cagttcaggg gttttatagg cagcaatacc gccgctaacg
    25681 ccgagaacga tttttttacc ggccaggctc atgatgattt ttcctgttgg gtgacaccag
    25741 aagttggcga ttttatcaca atacttttgt tgtcgtgcct tcacctgaga ttcactttgc
    25801 gaggcgcttt ccaggattga aaactggccg tcgatttaac ggaacggcta tgacaggatg
    25861 cgagcaccac aaaggaggtg aaggtgaaaa acaatgcaca gctgttgatg ccgcgcgaaa
    25921 aaatgctgaa gtttggtatt agcgccttaa ctgatgtcga gctgctggcg ctatttctgc
    25981 gtaccggaac gcgcggtaaa gatgtattaa ccctggcaaa agagatgctg gagaatttcg
    26041 gctctcttta tggcttgtta acctctgaat atgaacaatt tagtggtgtt catggaattg
    26101 gcgtagcgaa atttgcccag ttaaagggga ttgctgaact ggcgcgacgt tactacaacg
    26161 tgcggatgcg tgaagaaagc cctttactca gcccggagat gacgcgggaa tttttacaaa
    26221 gccagctcac gggtgaggag cgggagatct ttatggtgat ctttctcgac tcccaacacc
    26281 gggttataac gcatagccgt cttttttccg gcacgctaaa ccatgttgaa gtccatcctc
    26341 gggaaattat ccgcgaagcg ataaaaataa acgcctcggc gctgatcctt gcacataatc
    26401 acccttcggg ttgtgctgaa cccagtaaag cggataaact cattactgaa cggataataa
    26461 agagttgtca gttcatggat ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt
    26521 atgtttcttt tgccgaacgc ggctggattt aacccgctat gcgcgatcct tcgggatctt
    26581 tgtctgttcg ggacttgagc acatcgctga gtcagcgtat actacgccac ctttgagaat
    26641 ctcgggtttg gcatttgggc ctggcaatcg agagttcaca tagaactgcg atgaccgggc
    26701 tgtaaagcct gacgaggcgc caatacccca tacgaagctc gagctaattt gatttttgga
    26761 gaatagacat gtcccgagtc tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc
    26821 gttcccacgc actgaacgcg actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt
    26881 tctgggttga gagcgagaag cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg
    26941 taatcgataa aaaaggcatc gatacagttc tggctgaact gcgtgcccgt ggcgaaaagt
    27001 actaagtact tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt
    27061 ttcttctgct ggtactggtc acttctatac cactacgaag aacaaacgta ctaagccgga
    27121 aaaactggaa ctgaaaaaat tcgatccagt tgttcgccag cacgtgatct acaaagaagc
    27181 gaaaatcaaa taattctcgc tttgatgtaa caaaaaacct cgctccggcg gggttttttg
    27241 ttatctgctt gcccccatat tgactgcatc tgttcattcc tggagatgct atgcctgaat
    27301 tacccgaagt tgaaaccagc cgccgcggca tagaaccgca tctcgttggt gcaaccattc
    27361 ttcatgcagt ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag atctaccgtt
    27421 taagcgacca accagtgctt agcgtgcagc ggcgggctaa atatctgctg ctggagctgc
    27481 ctgagggctg gattatcatt catttaggga tgtctggcag cctgcgcatc cttccagaag
    27541 aacttccccc tgaaaagcat gaccatgtgg atttggtgat gagcaacggc aaagtgctgc
    27601 gctacaccga tccgcgccgc tttggtgcct ggctgtggac caaagagctg gaagggcata
    27661 atgtgctggc ccatcttgga ccggagccgc ttagcgacga tttcaatggt gagtatctgc
    27721 atcagaagtg cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat aacaagctgg
    27781 tggtaggggt agggaatatc tatgccagcg aatcactgtt tgcggcgggg atccatccgg
    27841 atcggctggc gtcatcactg tcgctggcag agtgtgaatt gttagctcgg gtgattaaag
    27901 cggtgttgct gcgttcgatt gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg
    27961 atggtaaacc gggctatttc gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt
    28021 gtcgggtgtg cggtacgccg attgtggcga ctaaacatgc gcagcgggca acgttttatt
    28081 gtcggcagtg ccagaagtaa ttcatgcgcg ccggatggca taccatccgg cataaacgct
    28141 acgctaactt cgccatcagc gcctgatgga cattctccgg caggaaatgg gtgacatcgc
    28201 cctgatggcg cgccacctct ttcaccaacg atgaagagat aaacgaccac tctttcgacg
    28261 gcatcagaaa cacactttcc agttccggca ttaagtggcg attcatatgc gccagctgca
    28321 tttcatattc aaaatctgcc accgcacgca ggccacgaat cagcaccgta gcgtgttgat
    28381 tacgggcgaa gttcgccatc aaatcactaa acccgaccac ttccacgttc cccagatgcg
    28441 cggttgcctg ctgtgccagt gccacacgct cttccagggt aaacatcggt tttttactgg
    28501 ggctggcggc aatcgccaga ataacgtgat cgaacatctg cgtggcgcgc gtcacgatat
    28561 cgatatgacc attggtaatg ggatcgaaag tacccggata aatcgcccgt ttttgcataa
    28621 caacctcaat gcgttttcgg tggcaggtaa ggttccagca gttgaagcag acgctgtagc
    28681 gcgccctggt tttgatacag tacttcaacg gcatgacggc catagaaact acggtaatcg
    28741 gcgtcggtga gtaaagagga aacctctttt gcaagcgtag tggcatcggt aacggtaatc
    28801 agcccgcttg cctgctccag ccgcgcgcaa atgtctttaa agttaaaagt atgcggcccc
    28861 atcaataccg gaattgcgtg tgcggcagct tccagcggat tatgcccacc acgttcaacc
    28921 agtgaaccgc caacaaaggc gagatcggca atgccgtaca gtaacatcaa ctcgcccatc
    28981 gtatcgccaa ccacaacctg cgtgctggtg gaggggactt cccctgaaga gcgtgtgata
    29041 tagcttagtc cagcctggcg gacaaggtta atcgcatccg ggaagcgttc tggatgacgg
    29101 ggtaccagga tgagcaataa attcgggaat tgctgtaata aagcctgatg tgcggcaatg
    29161 accacgctct cttcaccttc gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc
    29221 cactggcggc gcagcgtcac ggctttagca gccaactgcg gcgttacaga aatatcgaat
    29281 ttcaggctac cggtaacggt tacctggtta tttttcgcgc ccagcgccac aaaacgtgca
    29341 ccatcttctt cattttgggc ggcaatcagc gtaatacgac gcagcaagcg acggacgaat
    29401 ttacccagtt tggcataacc tgcggccgag cgggcagaga gtcgcgcgtt agcgatcacc
    29461 agcggaattt tacgtttatg tagcgccgca atcaggttag gccatagttc ggtttccata
    29521 atcaacacca gtttagggtc gactttattc aggaaacggt tgagtgcatc gggcagatca
    29581 tacggcagat aaacgtgctg aacatccttc ccgaaagccg attgtacgcg ctccgaaccg
    29641 gttggcgtca tggttgttac ggtaataggt aaatcaggat aacgatgacg cagcgcgcgc
    29701 accaacggga tcgccgccag agtttcaccg acggagacgg agtgcagcat aatgccgcct
    29761 ggttttagcg gatggcggta aaaaccgtaa cgttcacccc agcgttttcg ataggccgga
    29821 gccttacgtc cgcgcaccca gagccgtatc cagatcagcg gctgaataag gtagagaagg
    29881 gcggtgtaaa gcaattcgag catagtaaat agctgactta tggatgtgct ggggattcta
    29941 tgtatttagc tgtggcttta ccattacttt tcccgttttt gacttaaata gcaatttacc
    30001 cgccaattct tgctgaacat ctatactttt tttgtatttt tatgcgattc attgatttct
    30061 gctgtacgtg caggaaggaa tcgcagagca acagtgaaag aatatggttg ctaatgattt
    30121 tatttgattg atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat
    30181 tgccgcgaaa gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta
    30241 gctgttgagc ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa
    30301 caaaaagagt tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt
    30361 catggggata tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac
    30421 gcaaaaatcg atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag
    30481 attaacgcgc tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac
    30541 ttttttcatc tcatcaaggt attacgtgcc aataagtatg accttatcgt caatcttacc
    30601 gatcaatgga tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag
    30661 gattatcatc atcggcagtc tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg
    30721 cagggtggaa atgtggtgga aagtaactta tccgtgctga caccattggg acttgattcg
    30781 ttggtgaagc agacaaccat gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa
    30841 cttgatcacg ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc
    30901 ttcaaatgct gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt
    30961 tatgaagtcg ttctgacgtc cggcccagat aaagacgatc tggcctgcgt caatgaaatt
    31021 gcgcaaggat gccagacgcc accagtaacg gcgctggctg gaaaggtgac cttcccggaa
    31081 cttggtgcgt taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat
    31141 attgccgctg cagttaatac gccgctgata tcgctgtttg gtgcgacaga ccatattttc
    31201 tggcgtcctt ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca
    31261 acgcgcgatc agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc
    31321 attgctgctg tcgataaatt actgccctcc tccacgacag gtacgtcgtt atgatcgttg
    31381 ctttttgttt atataaatat tttccctttg gcggtttgca gcgcgatttt atgcgtattg
    31441 ctcagacagt cgccgcccga ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg
    31501 aatgccctga tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc
    31561 gcaatgcgga gtattttgcc tgggtgcaaa aacatttacg cgaacatccc gtcgataaag
    31621 tcgttggatt caacaaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg
    31681 ccgagaaagt cgcgcaggaa aaaggctttt tctatcacct gacgtcacgt tatcgccatt
    31741 atgccgcctt tgagcgggca accttcgaac agggcaagcc gacacagctg ctgatgctga
    31801 cagataagca aatcgccgat ttccagaaac attatcagac tgaagcggag cgttttcata
    31861 ttctaccacc ggggatttat cctgatcgta aatatagcca gcagccagct aatagccgtg
    31921 aaatcttccg taagaagaat ggaataaccg aacaacaata tttattgttg caggtcggtt
    31981 cagacttcac gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt
    32041 cgctgcgcca caacacattg ctatatgttg ttggacagga taaaccgcga aaatttgagg
    32101 cgctggcaga aaaacgcggc gtgcgcagta atgttcactt cttctcgggg cgcaacgatg
    32161 tctcggaatt aatggcggcg gcggatttat tactgcatcc tgcctaccag gaagcagcgg
    32221 gaattgtgct actggaagcg attactgcag gattaccggt actaacaaca gccgtttgtg
    32281 gctatgcgca ttatattgtc gacgccaatt gcggcgaggc tattgctgag ccattccgcc
    32341 aggaaacatt gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct
    32401 gggcggaaaa tgcgcgacat tatgctgata cacaagattt atacagtctg ccagagaaag
    32461 cggcggatat cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta
    32521 tggcgcggta aagatccttt tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa
    32581 ctggaaactc gccgcactct gcgatttgaa atggcgggca aaagctattt tctcaaatgg
    32641 catcgcggca cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta
    32701 ttaggcgcag accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact
    32761 atgtatgggg tggcatttgg cgaaaaaggc atgaatccgc tgaccagaac ttcatttatt
    32821 attaccgaag atctgacacc aaccataagt cttgaagatt actgtgctga ctgggcgact
    32881 aaccctccag atgttcgcgt aaagcgtatg cttattaagc gtgtcgcgac gatggtgcac
    32941 gatatgcatg ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac
    33001 ttgccttttt ccggtaagga agaggagtta aaaatttcgg taattgacct gcaccgggcg
    33061 cagcttcgca cgcgcgttcc acgtcgttgg cgggataaag atcttattgg gctttatttt
    33121 tcttcgatga atatcggcct gactcagcgg gatatctggc ggtttatgaa agtgtatttt
    33181 gccgccccgc ttaaagacat tctcaagcag gaacaaggac tgctgtcgca agcagaagca
    33241 aaagccacaa aaatcaggga aagaacgatt cgaaaatcgt tgtaacccgt aacttatttt
    33301 tgccaaa
//
LOCUS       DAZFKY010000032        32734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFKY010000032 DAZFKY010000000
VERSION     DAZFKY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..528)
                     /gene="lacA"
                     /locus_tag="ACHKCL_001364"
     CDS             complement(<1..528)
                     /gene="lacA"
                     /locus_tag="ACHKCL_001364"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO5762366.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAG"
     gene            complement(594..1847)
                     /gene="lacY"
                     /locus_tag="ACHKCL_001365"
     CDS             complement(594..1847)
                     /gene="lacY"
                     /locus_tag="ACHKCL_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5762367.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(1899..4973)
                     /gene="lacZ"
                     /locus_tag="ACHKCL_001366"
     CDS             complement(1899..4973)
                     /gene="lacZ"
                     /locus_tag="ACHKCL_001366"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5762368.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(5096..6178)
                     /gene="lacI"
                     /locus_tag="ACHKCL_001367"
     CDS             complement(5096..6178)
                     /gene="lacI"
                     /locus_tag="ACHKCL_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5762369.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(6215..7168)
                     /locus_tag="ACHKCL_001368"
     CDS             complement(6215..7168)
                     /locus_tag="ACHKCL_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO5762370.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(7188..7964)
                     /locus_tag="ACHKCL_001369"
     CDS             complement(7188..7964)
                     /locus_tag="ACHKCL_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO5762371.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(8079..8912)
                     /gene="mhpR"
                     /locus_tag="ACHKCL_001370"
     CDS             complement(8079..8912)
                     /gene="mhpR"
                     /locus_tag="ACHKCL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO5762372.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            9103..10767
                     /gene="mhpA"
                     /locus_tag="ACHKCL_001371"
     CDS             9103..10767
                     /gene="mhpA"
                     /locus_tag="ACHKCL_001371"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO5762373.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            10769..11713
                     /gene="mhpB"
                     /locus_tag="ACHKCL_001372"
     CDS             10769..11713
                     /gene="mhpB"
                     /locus_tag="ACHKCL_001372"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO5762374.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            11731..12597
                     /gene="mhpC"
                     /locus_tag="ACHKCL_001373"
     CDS             11731..12597
                     /gene="mhpC"
                     /locus_tag="ACHKCL_001373"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO5762375.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            12607..13416
                     /gene="mhpD"
                     /locus_tag="ACHKCL_001374"
     CDS             12607..13416
                     /gene="mhpD"
                     /locus_tag="ACHKCL_001374"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO5762376.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            13413..14363
                     /gene="mhpF"
                     /locus_tag="ACHKCL_001375"
     CDS             13413..14363
                     /gene="mhpF"
                     /locus_tag="ACHKCL_001375"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO5762377.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            14360..15360
                     /gene="mhpE"
                     /locus_tag="ACHKCL_001376"
                     /pseudo
     CDS             14360..15360
                     /gene="mhpE"
                     /locus_tag="ACHKCL_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
     gene            15536..16747
                     /gene="mhpT"
                     /locus_tag="ACHKCL_001377"
     CDS             15536..16747
                     /gene="mhpT"
                     /locus_tag="ACHKCL_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5762378.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            16849..17388
                     /gene="yaiL"
                     /locus_tag="ACHKCL_001378"
     CDS             16849..17388
                     /gene="yaiL"
                     /locus_tag="ACHKCL_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5762379.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDNPYADFKVPDDLMW"
     gene            complement(17513..18346)
                     /gene="fghA"
                     /locus_tag="ACHKCL_001379"
     CDS             complement(17513..18346)
                     /gene="fghA"
                     /locus_tag="ACHKCL_001379"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5762380.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(18439..19548)
                     /gene="frmA"
                     /locus_tag="ACHKCL_001380"
     CDS             complement(18439..19548)
                     /gene="frmA"
                     /locus_tag="ACHKCL_001380"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5762381.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(19583..19858)
                     /gene="frmR"
                     /locus_tag="ACHKCL_001381"
     CDS             complement(19583..19858)
                     /gene="frmR"
                     /locus_tag="ACHKCL_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5762382.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(20018..21064)
                     /gene="fbpC"
                     /locus_tag="ACHKCL_001382"
     CDS             complement(20018..21064)
                     /gene="fbpC"
                     /locus_tag="ACHKCL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO5762383.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(21076..23154)
                     /locus_tag="ACHKCL_001383"
     CDS             complement(21076..23154)
                     /locus_tag="ACHKCL_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5762384.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(23223..24254)
                     /locus_tag="ACHKCL_001384"
     CDS             complement(23223..24254)
                     /locus_tag="ACHKCL_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5762385.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(24251..25567)
                     /gene="uhpC"
                     /locus_tag="ACHKCL_001385"
     CDS             complement(24251..25567)
                     /gene="uhpC"
                     /locus_tag="ACHKCL_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5762386.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(25640..27181)
                     /locus_tag="ACHKCL_001386"
     CDS             complement(25640..27181)
                     /locus_tag="ACHKCL_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5762387.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(27181..27810)
                     /locus_tag="ACHKCL_001387"
     CDS             complement(27181..27810)
                     /locus_tag="ACHKCL_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO5762388.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            28113..29075
                     /gene="tauA"
                     /locus_tag="ACHKCL_001388"
     CDS             28113..29075
                     /gene="tauA"
                     /locus_tag="ACHKCL_001388"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5762389.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFSKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            29088..29855
                     /gene="tauB"
                     /locus_tag="ACHKCL_001389"
     CDS             29088..29855
                     /gene="tauB"
                     /locus_tag="ACHKCL_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5762390.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            29852..30679
                     /gene="tauC"
                     /locus_tag="ACHKCL_001390"
     CDS             29852..30679
                     /gene="tauC"
                     /locus_tag="ACHKCL_001390"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5762391.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            30676..31527
                     /gene="tauD"
                     /locus_tag="ACHKCL_001391"
     CDS             30676..31527
                     /gene="tauD"
                     /locus_tag="ACHKCL_001391"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5762392.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(31634..32608)
                     /gene="hemB"
                     /locus_tag="ACHKCL_001392"
     CDS             complement(31634..32608)
                     /gene="hemB"
                     /locus_tag="ACHKCL_001392"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5762393.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         7678 a         8633 c         8970 g         7453 t
ORIGIN      
        1 gccagccgcc acgacgtttg gtggaatgtc ttttgtgacg atactacccg cgccaataac
       61 agaattatcc ccaatggtga cgcctggatt aataaccaca tgacttccga tccagacgtt
      121 attgccaatc gttatcggaa aagagtacat ctcaccgttt tttctcaatt catggtgtac
      181 agggtgtccc gtaacggaaa gagtaacgtt gggtgcaatc aatacgttat caccgattgt
      241 taccgtgtag tcatcgacaa tggttaaatt gaaatttgca taaaaattgc ggcctatatg
      301 gatgttggaa ccgtaagaga aatagacggg gggttctacc caggcgtttt cccctaccgt
      361 ggcaaacatt tctttaatca ggctttctct tttatcaact tctgatggat gcgagtgatt
      421 aaactcatac attaacgttt ttctacgaag tcttttttca ggtaagcctt cgcacatatc
      481 ggtaaatagc ttgcctgctt ttattctttc tgtcatcgac atgttcattg cgatcattcc
      541 gttatgatat gttggtcgga taaggcgctc gcgccgcatc cgacattgat tgcttaagcg
      601 acttcattca cctgacgacg cagtagagaa agcgggccgg ggccgctaag cgtgaacacg
      661 gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc ctggaaaccg
      721 atgctttcat acatattacc cgctagtaca gacataaaaa tcatcgccag ttgcttaaag
      781 aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg gctggtaata
      841 tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt tttcagaata
      901 accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga cataatagtg
      961 ccagccagca gcagggcatt tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac
     1021 ataattgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc
     1081 tgttcaccgg tggcaaagaa agaagtaaag aaattagcaa actgttggtc aaaaacatcg
     1141 taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg ctgtctgaac
     1201 agttccagcg ccagtttaag gctaaatgcc gaatggttgg cacctaccgc attggcaacc
     1261 gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaatggcgag gatgagtgca
     1321 cagccagaac ccagccagaa aacgaactga ttattgatgg tgaacatgat gccgacaatc
     1381 gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta
     1441 ctgcgacggc tgactttctc gataaatgcc tctactgcgg gcgcaccggc gttaaaacaa
     1501 aagccaagat aaataccacc aacaatcgat cctactaaaa tgttgtattg taacagtggc
     1561 ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat aatccacagc
     1621 aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg gaataatagc
     1681 gagaacagag aaatagcagc aaaaataata cccgtatcac ttttgctgat atggttgatg
     1741 tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa gtaaaagaaa
     1801 aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat ggatttcctt
     1861 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     1921 tggtaatggt agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag
     1981 tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgttcc ttcttccgcg
     2041 tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg gctgatgttg
     2101 aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc
     2161 agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag
     2221 cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc
     2281 cagtttaccc gctctgctac ctgcgccagc tggcaggtca ggccaatccg tgccggatgc
     2341 ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc
     2401 cggtaggttt tccggctaat aaataaggtt tttccctgat gctgccatgc gtggacagtg
     2461 gtaatcagca ccgcgtcggc aagtgtatct gccgtgcact gcaacaacgc cgcttctgcc
     2521 tggtaatggc ccgcagcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct
     2581 tcacttacgc caatgtcgtt atccagcggt gcgcgggtga actgatcgcg cagcggcgtc
     2641 agcagttgtt ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat
     2701 tgccaacgct tattatccag ctcgatgcaa aaatccgttt cgctggtggt cagttgcggg
     2761 atggcgtggg gcgcggaggg gagtgtcacg ctgaggtttt ccgccagacg ccactgctgc
     2821 caggcgctga tgtgtccggc ttctgaccat gcggtcgcgt tcggttgcac tacgtgtacc
     2881 gttagccaga gttgcccggt gctctccagt cgcggtagtt caggcaattc aattacctgt
     2941 ttaccttgcg gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc
     3001 gccaccgtcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact
     3061 tcgatggttc gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc
     3121 agcgccggat gcggcgtgcg gtcggcaaag accagaccgt tcatgcagaa ctggcgatca
     3181 ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg gattgccatt ttcatcatat
     3241 ttaatcagcg actgatccac caagtcccag acgaagccgc cctgtaaacg ggggtactga
     3301 cgaaacgcct gccagtattt agcgaagccg ccaagactgt tacccatcgc gtgggcgtat
     3361 tcgcaaagga tcagtgggcg catttctcca ggcagcgaaa gccatttttt gatggaccat
     3421 ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata
     3481 tcggttgcgg aggtgtcggc tccgccgcct tcatactgca ccgggcggga tggatcgaca
     3541 gatttaatcc agcgatagag tgcgtcgtga ttagcgccgt ggcctgactc attccccagc
     3601 gaccagatga tcacactcgg gtgattacga tcgcgctgta ccattcgtgt tactcgttcg
     3661 ctcatggccg gtaaccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg
     3721 gtttcaatat tggcttcatc caccacatac aggccgtagc ggtcgcacag ggtgtaccac
     3781 agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcattagc
     3841 aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg
     3901 tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag accattttca
     3961 atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg
     4021 gtgtgcagtt caaccaccgc acgatagata ttcgggattt cggcgctcca cagtttcggg
     4081 ttttcgacgt tcagacctag tgtgacgcga tcggcataac cgccacgctc atcgataatt
     4141 tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca caaagaaacc
     4201 gtcacccgca gctcatcgcg cagctcgccg tacatctgaa cttctgcctc cagtaccgcg
     4261 cggctaaaat catcattaaa gagagtggca acatggaaat cgctgatttg tgtggtcggt
     4321 ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc
     4381 agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt
     4441 aaaaatgcgc tcaggtcaaa ttcagacagc aaacgactgt cctggccgta accgacccag
     4501 cgcccgttgc accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg
     4561 ccttcctgta gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc
     4621 tccgtgggaa caaatggcgg attgaccgta atgggatatg tcacgttggt gtagatgggc
     4681 gcgtcgtaac cgtgcatctg ccagtttgag gggacgacga cagtatcggc gtcaggaaga
     4741 tcgcactcca gccagctttc cggaaccgct tctggtgccg gaaaccagac aaattgccac
     4801 tcaccattca ggctgcgcaa ctgctgggaa gggcgattgg tgcgggcctc ttcgctatta
     4861 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4921 tcccagtcac gacgttgtaa tacgacggcc agtgaatctg taatcatagt catagctgta
     4981 tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg gacgcataga
     5041 gtgtaaagcg tggggtgcct aatgagtgag ctgactcaca ttaattgcgt tgcgctcact
     5101 gcccgctttc cagtcgggaa acttgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5161 ggggagaagc ggtttgcgtc ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
     5221 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
     5281 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacacg
     5341 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcaacccgg
     5401 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
     5461 tgggaacgat gccctcattt agcatttgca tggtttgttg aaaaccggac atggcactcc
     5521 agtcgccctc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
     5581 ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
     5641 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
     5701 taatactgtt gatgggagtc tggtcagaga catcaagaaa taacgccgga acattagcgc
     5761 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
     5821 tgacgcgttg cgcgagaaga ttgtgcactg ccgctttaca ggcttcgacg ccgcttcgtt
     5881 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
     5941 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
     6001 gtttccccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
     6061 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctgattcacc acgcgggaaa
     6121 cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact ggtttcacat
     6181 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
     6241 gccattcggc ggggctgacg ccgaagtgag atttaaaatg ctgacgccag gtcacaggtg
     6301 agagaaaacc cacctcagcc gctatccgct caacgggcaa ctgactggat cccaacagtt
     6361 cctggctacg gcgtaagcgt tcagtaatga gccattcggc gatactcgaa ccggtcgctt
     6421 tcataaaatg gcgagttaat gtgcggcgac tcatcattac tcgctgcgcc agcgagtcga
     6481 gatcatgctg ttgcgcaatg ttttgccgca ggtaatccag caggaggttt atgcgttcat
     6541 catgggtatt tttcggcacc ggttgctcaa taaactgcgc ctgaccgccg gtgcgatatg
     6601 gcggtacgac catccgtcgg gcaatatggt tagcatagtc gctgccaaaa tgttgccgaa
     6661 caatatacaa acagcaatcc agcgccgcgg cagtaccggc agaggtaata atgccgtcat
     6721 cgtctacgta caacgcgtta atatccagat gtgtttgtgg aaaacgtgcc tgaaattcac
     6781 gctcgaactc ccagtgcgtg gcggcacgct tatttttcag taaacctgca tagccgagca
     6841 caaacgtgcc caggcaaagc ccgacaatct gtgcgccgtt atcccgtgcg gtcgttaagg
     6901 cttccagcag tttttgtggc ggtttttgtg taatggttcc ccagtaagga attatcacaa
     6961 tatctgcctg gatgactgcg gtatagtcat gggtggcggt tacggaaaac ccatcggcag
     7021 agtccacgtt tcccggcttt tcagcgcaaa taatcacgtg aaagcgtttt ttctccgcca
     7081 tcttttcact aaacacgata aacggtacgg aaaggtgaaa aggactaaaa ccggcaacgg
     7141 cgataatggc aacagttagc gggcgcataa cactctttat ctccgaatta gaatgtcaac
     7201 gtttcaccat cttctggtac gttcacaaat tcgataattt gattatctct ggcgtattcc
     7261 ttgagcgcat cacgcgtcag caaacagtgg tttacggcct ccatatgtgt cgcgataatt
     7321 tgcgcttctg ggagggtaaa atgtgttttg agaatatctt ctgctcccat aattatcgcg
     7381 ccatgcccca gaacatgcgc aaatccggca ttcaacacaa ccacttctgg ttggaaagtt
     7441 agcattgtag cctcgacgtc gtcacgccag agggtatcgc cagccagata gagcgttttc
     7501 tctgtcgggt gctggaaaac aacgccgcaa gctgcgccta aacgcttcgc catcgccggg
     7561 tcggcatagg tgcgatcgga gccgtgctgc cctgtcgttt tatgcagagt aatatcgcca
     7621 aatttagtct cgtcggtaag tacggtaaga tgcgtaaatc cttgtttatt cagtgattca
     7681 gcatcttgct cattttgcac ataaatcagt ttatctttag ggacgacgcg ggaggctgtt
     7741 tcgtcccagt gatcttcatg caaatgggtc acgattaggg cgtcaacgtt gagtaacgtt
     7801 gttaaatcgc acggtaattc taccgttgga ttgcggattt ctgcgcgtgc cgttccggca
     7861 aaacccggat aacttccttt aggcgcgagt aacgggtcga taagaaatgt tttgccagcg
     7921 tattggataa tttgggtggc attacgaatt tgcgtaattt tcatgatttg ctccacatca
     7981 cagtattgat gtcattagcg tagcgtgcag cccgtactgt gcatgtgggc aaatgggcga
     8041 ataacgatga gaatgggcca aattacacat tggcgtaact agcgtaaatg cacgccgctt
     8101 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
     8161 cgttgtagtg ccggaagatg cttttccgct gcctgttcga tggtcattgc gctcgccata
     8221 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
     8281 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
     8341 cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc atcaccgggg
     8401 cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa ggccagccag
     8461 gtcaggcccg aggcggtttt cagaagcggt aaacgccgcc cgaccattgc ccggtgaaag
     8521 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
     8581 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
     8641 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga
     8701 cgaaaactat catcggaggg actacgccgg acatatccct cttcctgcag cgtctccagc
     8761 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
     8821 ccgccgtcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
     8881 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
     8941 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa caccctttct
     9001 atactgagcg cacaataaaa aatcatttac atatttttaa caaaataagt tgcgctgtac
     9061 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
     9121 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgctggtgc cggtccggtc
     9181 gggctgatga tggcgaacta tctcggccag atgggcattg acgtgctggt ggtggagaaa
     9241 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
     9301 atgcagtcgg tcggcctggt cgataatgtt ctgccgcata ctacgccgtg gcacgcgatg
     9361 cgttttctca ccccgaaggg ccgctgtttt gctgatattc agccaatgac cgatgaattt
     9421 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
     9481 gtgtcgcgtt ttccgaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagtcag
     9541 caagatgacg aagtgacctt gcacctgaaa acggcagaag ggctgcggga aatagtcaaa
     9601 gcccagtggc tggtggcctg tgatggtggg gcaagttttg tccgtcgcac cctgaatgtg
     9661 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
     9721 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
     9781 ctacctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagaacag
     9841 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
     9901 gttgaattga ttcgccagcg tgtctacacc cacaacgcgc gactggcgca acgtttccgt
     9961 attgatcgcg tactgctggc gggcgatgcc gcgcacatca tgccggtatg gcaggggcag
    10021 ggctataaca gtggtatgcg cgacgccttt aacctcgcat ggaaactggc gttggttatc
    10081 caggggaaag cccgcgatgc gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    10141 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    10201 cagggtacgt tacgtgacgg cgtttcctgg ctgctgaatt atctgccgcc agtaaaacgc
    10261 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtgcgt
    10321 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    10381 gaaaacggcg acgtgacgct gctcgataac gcgatcggcg cgaacttcgc ggtaattggc
    10441 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    10501 agcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    10561 gacggcgtac tacgcgtggg ggatacgcaa ggtcgcctgc gtagctggtt cgcgcaacac
    10621 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    10681 acactgggca agaccctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    10741 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcattgtc tttcccactc
    10801 gccgctggtg gggtatgtcg acccggcaca agaggtactc gatgaggtca atggcgtgat
    10861 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    10921 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    10981 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    11041 ggaggcctgt gcgcatgccg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    11101 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    11161 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    11221 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    11281 cctaggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    11341 ccatatgcgc gaccgcctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    11401 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    11461 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    11521 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    11581 aatcaaaacc tgggtcgccg cttttgccgc tatttctgca tttggcaact ggcgtagcga
    11641 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    11701 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    11761 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    11821 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    11881 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtgatcctg
    11941 ctggattgtc cgggttgggg caagagcgat tcgatcgtta atagtggttc gcgatcggat
    12001 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    12061 ctgggcaact cgatgggtgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    12121 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    12181 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    12241 aagctgatga tggatatctt cgtttttgat accagcgatt tgaccgacgc cctgtttgaa
    12301 gcgcgcctga ataatatgct gtcgcgccgc gatcacctgg aaaacttcgt taagagcctg
    12361 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    12421 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    12481 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtgggca ttgggcgcag
    12541 tgggaacatg ccgacgcttt caatcaactg gtgctgaatt tcctcgcacg tgcttaagga
    12601 acggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    12661 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    12721 tacgccattc agcacataaa tgtgcaatat gacgttgcgc aggggcgtcg cgtggtgggg
    12781 cgtaaagtgg gcctgacaca tccgaaagtg cagcaacaac tgggcgttga tcaaccggat
    12841 ttcgggacat tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    12901 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    12961 actgatatca ccttcgacga attgtataac gccattgaat gggtacttcc ggcgctggag
    13021 gtggtgggta gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    13081 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    13141 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    13201 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    13261 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggtcc gatggtggca
    13321 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcggca
    13381 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    13441 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacgat cagcatctgg
    13501 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgcgt gccagacgta
    13561 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    13621 atatcgacat tgtatttgat gcgaccagcg ccggtgctca tgtgaaaaac gatgcggctt
    13681 tgcgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    13741 actgcgtgcc ggtggttaac ctcgaggaga acgtcgatca actgaacgtc aacatggtca
    13801 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gtgcgtgttc
    13861 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    13921 atatcgatga atttacggaa accacttcgc gagccattga agtggtgggc ggcgcggcaa
    13981 aagggaaggc gattatcgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    14041 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    14101 ctgaggcggt gcaggcttac gtaccgggat atcgcctgaa acagcgcgtg cagtttgaag
    14161 ttatcccgca ggataaaccg gttaatttgc cgggcgtagg gcagttctcc gggctgaaaa
    14221 cggcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    14281 tcgacattat gacttccagt gcgctggcga cagcagaaaa aatggcccag tcactggcgc
    14341 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    14401 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    14461 agcactggac gatgcccgcg tggattcgat tgaagtggcc cacggcgacg gtttgcaggg
    14521 gtccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    14581 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    14641 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggcta cgcactgtac
    14701 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    14761 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    14821 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatatccgt
    14881 gaccgtttcc gcgccctgaa agcagtgctg aaaccagaaa cgcaaaccgg catgcacgct
    14941 caccataacc tgagtcttgg cgtggcgaac tctatcgcgg cggtggaaga gggctgcgac
    15001 cgaatcgacg ccagcctcgc gggaatggga gcgggcgcag gtaacgcgcc gctggaagta
    15061 tttattgccg ccgtggataa actgggttgg cagcatgggg ccgatctcta tgcgttaatg
    15121 gatgctgctg acgacctggt gcgtccgttg caggatcgac cggtacgagt cgatcgcgaa
    15181 acgctggcgc tgggatacgc tggtgtttac tccagcttcc tgcgtcactg tgaaacggcg
    15241 gcggcgcgtt atggcttaag cgcggtggat attctcgttg agctgggcaa acgccggatg
    15301 gttggcggcc aggaggatat gatcgtcgac gtggcgctgg atctgcgcaa caacaaataa
    15361 tattgactgc cgagaacgtg cattttgtat gcccatccca gcacgactgc cggatgcggc
    15421 gtgaacgcct tatccggtct acatttcgcc cgtaggcagt cattaaatag ttctgattac
    15481 gcggctggca atgtatcagt cgcgattcac ctgcacacgc aatgaggttc atcttatgtc
    15541 gactcgtacc ccttcatcat cttcatcccg cctgatgctg accatcgggc tttgtttttt
    15601 ggtcgctctg atggaagggc tggatcttca ggcggctggc attgcggcgg gtggcatcgc
    15661 ccaggctttc gcactcgata aaatgcaaat gggctggata ttcagcgccg gaatactcgg
    15721 tttactaccc ggcgcgttgg ttggcggaat gctggcggac cgttatggtc gcaagcgcat
    15781 tttgattggc tcagttgcgc tgtttggttt gttctcgctg gcaacggcga ttgcctggga
    15841 tttcccctca ctggtctttg cgcggctgat gaccggtgtc gggctggggg cggcgttgcc
    15901 gaatcttatc gccctgacgt ctgaagccgc gggtccacgt tttcgtggga cggcagtgag
    15961 cctgatgtat tgcggtgttc ccattggcgc ggcgctggcg gcgacactgg gtttcgcggg
    16021 ggcaaactta gcatggcaaa cggtgttttg ggtaggtggt gtggtgccgt tgattctggt
    16081 gccgctatta atgcgctggc tgccggagtc ggcggttttc gctggcgaaa aacaggctgc
    16141 gccaccactg cgtgcgttat ttgcgccaga aacggcaacc gcgacgctgc tgctgtggct
    16201 gtgttatttc ttcactctgc tggtggttta catgttgatc aactggctac cgctgctttt
    16261 ggtggagcaa ggattccagc catcgcaggc ggcaggggtg atgtttgcac tgcaaatggg
    16321 tgcggcgagc gggacgttaa tgttgggcgc attgatggat aagctgcgtc cagtaaccat
    16381 gtcgctactg atttatagcg gcatgttagc ttcgctgctg gcgcttggaa cggtgtcgtc
    16441 atttaacggt atgttgctgg cgggatttgt cgcggggttg tttgcgacag gtgggcaaag
    16501 cgttttgtat gccctggcac cgttgtttta cagttcgcag atccgcgcaa caggtgtggg
    16561 aacagccgtg gcggtagggc gtctgggggc tatgagcggt ccgttactgg ccgggaaaat
    16621 gctggcatta ggcactggca cggtcggcgt aatggccgct tctgcaccgg gtattcttgt
    16681 tgctgggttg gcggtgttta ttttgatgag tcggagatta agaatgcagc cgtgtgccga
    16741 tgcctgacgc gccttattaa gcaggggcga aagggacttg cccccaatac acccacggcg
    16801 tttacaggta tactcgctaa aaattattca gcgggtttgg aaacaaagat ggcaaaactt
    16861 accttacaag agcagttgct caaagcagga ttagtcacca gcaaaaaagc ggcgaaggtc
    16921 gagagaacgg cgaaaaaatc gcgcgttcag gcgcgtgaag ctcgggcggc ggtagaagaa
    16981 aataaaaagg cacagcttga gcgtgataaa cagcttagcg aacagcaaaa acaagcggcg
    17041 ttggcgaaag aatataaagc tcaggtgaag cagcttattg aaatgaaccg gatcaccatt
    17101 gccaatggcg atattggttt taacttcact gatggcaatc tgattaagaa gatttttgtc
    17161 gataagctca cacaggcgca gttgattaat ggtcgtctgg cgattgcccg cttgttggtc
    17221 gataacaata gcgaaggtga atacgccatt attcccgcca gcgttgccga taaaattgct
    17281 cagcgtgatg ccagcagtat tgtgttgcac agcgcgctca gtgccgaaga gcaggatgaa
    17341 gacaacccgt atgccgactt caaagtgcct gatgatttga tgtggtaaac atagataagt
    17401 cgtgcgcaga tgcctgatgc gacgctatgc gcgtcttatc atgcctacgg cctgcgggcg
    17461 aaatgtaggc ggataaggcg ttcacgccgc atccggcagt cgtgcactat tatcaacgca
    17521 tattcagttt attggcgtgg taggcaatat gctcgccaat aaaactggag acaaaataat
    17581 agctgtggtc gtaaccctcg tgataacgga ttaacgtttt gatattcatc tcctggcaga
    17641 tcttttcaag attttgagtc cgcagctgtt ccgcgtaaaa atcatcactc aacccctgat
    17701 caaccatgat ttccgcaacg cgttgacctt gtgaaataag actcaccgga tggtaatcca
    17761 accaggcctc tttattttca ccaagatatg cggcaaaggc ttgctgtccc cacggtactt
    17821 gcgatgggga gacaatgggc gaaaacgccg agacgctgac atattcatct gggttacgta
    17881 acgccagcac cagcgcgccc agtccgccca tagaatgacc agagatagac tttttggccg
    17941 ttgccggaaa atgattcatc actaaatccg gcagctcgtt gcggatatag tcatacattt
    18001 tgtaatgttc attccacggc gcttgcgtcg cgttcaggta aaacccggca ccttgcccga
    18061 gatcgtaacg gtcggcatct gcgacatgac tgcctcgcgg actggtgtcc ggcgcaacaa
    18121 caataatgtt gtgctcagcc gcgtaacgct gcacccccga tttagtaatg aaattctgct
    18181 cattgcaggt caggcccgaa agccagtaca gcaccggtaa tttttcacct gcagcttttg
    18241 gtgggagata gacgccgaca ttcatttcac atttcagtga tgcggaataa tgccgataca
    18301 cattttgcca gccgccaaag ctggcatgtt tttcaatgag ttccatcagt cattatctca
    18361 cggttaatcc cccggacggc tgcccggaga gagtattaag aaccccgtct gaagtggacg
    18421 gggtaaacca gcgggaaatc agtaacgaat tacggttcga atggatttgc cttcatgcat
    18481 caggtcgaag gcgtcattaa tttcatccag gctcatggta tgcgtgacaa acggttccag
    18541 atcgatatca cctttcatcg catcttcaac catacccggt aactgggaac gacctttcac
    18601 gccgccaaac gcggaacctt tccatacgcg accagtgacc aactggaatg gacgggtgga
    18661 gatttcctga ccggaacccg cgaccccgat gatcaccgac tgaccccagc cgcggtgcgc
    18721 actttccagc gccgcacgca tcacgttgac attaccgatg cattcaaagg tatggtcgat
    18781 accccatttg ttgatatcca gcaggacatc tttaatcggt ttgtcgtagt cattcgggtt
    18841 aatgcagtcg gtggcaccga agcgacgcgc cagatcgaat ttcttcgggt tggtatcgat
    18901 agcgataatc cgtcccgctt tcgcctgacg cgcgccctga accactgcca gaccaatcgc
    18961 gccaagacca aacacagcaa cagaatcccc tggctggact ttagctgtgt tgtgtaccgc
    19021 gccaataccg gtggtcacgc cacagcccag caggcagacg tgttcatggt ttgcttctgg
    19081 attaattttt gccagagaca cttccgcgac tacggtgtat tcactgaatg tagaacaccc
    19141 catgtagtga taaagcggct gcccattgta agaaaaacgg gtggtgccgt ctggcatcag
    19201 gcctttaccc tgggtttcgc gaaccgcaac acagaggtta gttttgccag aacgacagaa
    19261 ctcacactcg ccgcactctg cggtgtaaag cgggatcaca tggtcgcctg gtttgacgct
    19321 ggttacgcct tcaccgactt caaccacaac gccagcccct tcgtgaccga gaaccaccgg
    19381 gaatacacct tccgggtcat cgccggagag ggtaaatgcg tcggtatggc aaacgccggt
    19441 atgggtgact ttaatcagca cttcaccttt tttcggtggt gcaacgtcaa tttcaacgat
    19501 ttccagcggt ttaccgggag caaatgcaac ggcagcacgt gatttcatct ctcgctcttc
    19561 ctcaatatgg taatagattc agctatttaa gataggcacg aaccagttca atagtgtcgt
    19621 caacggattg gctgacttcg cggctgtagc agtcatttcg gtcaaacgtt tcccggatat
    19681 ggctttcaag cacttctgcc atcagcccat tagccgcgcc ccgaacggca gcgatctgtt
    19741 ggagtatggc acggcattcg gcatcaccct ccagcgaccg ttccagagca tcaatctgcc
    19801 ctcgaatacg acgaactcga gtaaggacct ttttcttctc ttccggagta ctgggcattt
    19861 cgcacctcat catctgcacg caatatacta taggggggta tgatatatgt caatgcatac
    19921 ccccctatag tatataacaa tggagaataa ttgttgtccg aagcctaacc aggtgaacgg
    19981 tccctcaaga cagatgggga ccgtttatgt gctattttta tgccgcatcc gctaaaacaa
    20041 acatcccgta aggatggatt tcgaggtaat agtgctcacc aatatcgggc tgtaaacggg
    20101 tggcgtttac ttgtaataaa atctcctgtc catgccactc tacggtcact tcgtactgcg
    20161 gccccatgta ggcgacatgg cgaataacac aacgctgact ctcttcgccg tgattgctta
    20221 aggtgatggc ttccgggcga acgccgacgg tgccgctacc ttgcaccggg aaatgcgccg
    20281 cgcgcggcag gcgataaccg tagatatcga catactcttc gctaaagttc gccgggaaca
    20341 ggttggcgtc gcccataaaa cttgccataa agcgggaggc tggctgacga tagagatcct
    20401 gcggtgagcc aatctgcatg atgtgacctt tattcatcac cagcacagtg tcggaaaccg
    20461 cgaaggcctc actttgatcg tgagtcacgt agagcgacgt gatattaaac tgcttttgca
    20521 gctcgcggat cttatcgcgc atgctgcggc gcaggttggc atcgaggtta cttaacggct
    20581 catcaaacag cagcaccttt ggtttgagga tcaacgcgcg ggccagcgcc acgcgctgtt
    20641 gctgaccacc ggaaatctgg tcgacatagc ggtcctcgaa cccttccaga tccaccattg
    20701 ccagcgcctc tttcacccgc tgctttactt cgctacgcga cacgccgagc atctttaacc
    20761 cgtagccgac gttttcgccc agcgacatat gcgggaacag agcgtatgac tgaaacacca
    20821 tgcagatatc gcgctgctga atggaacgat gcgtgacatc ttcgccatca ataaagattt
    20881 gcccttcact cggtttttcc agcccggcaa ccagacgcaa aatggtggtt ttgccgcagc
    20941 cggaagggcc aagcagcgtc accatttgcc cttgtgggat ggtgagattg atattgtcga
    21001 taaccgtatt actgccgaat cgtttagtga cgttgcgcag ttccacaaaa tttttctgac
    21061 tcatagtgcg ctccattacg cctggttttt ggctttagaa cgggagatac gtgcttctcc
    21121 gatcagccag tcaaagagga aaataatcgc cagcatcacc acgatcagaa tcgaaccata
    21181 ggcgatggct acgccatatt cgccgtcttc aacgcggtta agaatgtagg cggttgccac
    21241 gcgggtgtcc ggcgtgacga gaaagacgat ggcgctgacc gtcgtgatgg cccgcacaaa
    21301 gctgtagatc agggccgaga gaatggccgg acgcagcagc ggcagtaaaa tgtgggttat
    21361 ggttcgcaag gaacccgcgc gcaggctgag tgaggcttca tccagcgatt tgtcgatctg
    21421 ccccaggcct gcaatcccgg cgcgaatacc caccggaacg ttacgcatca ccatcgagat
    21481 aatcacaatt gccgccgttc cggtgagata aaccggagcg ctgttaaagg caagaatata
    21541 cgaaacgccc gccaccgtac ccggtacggc aaagcacagc atggtggtga actcgatggt
    21601 ctttttgcct ttgaattgtt gacgcaccac cacccaggcg atgagcaggc caaagatagc
    21661 ggtaatggga gcggcaatcc cggcgtagag cagggtatcc agcagtgaag gccacgcgcc
    21721 gtcgctcatt ccctgaccaa atagcttgat aaagttatcc agcgtcaggg tgtagtccac
    21781 gccccagttc accgtgaagc tgccgtagaa aatgctgccg tagagcaggg cgttaaaggc
    21841 aatccatatc gcaagtatgg cgaccacgct ccacaccagg gtgaccggaa gcggctggac
    21901 gtcgccacgg taggatttgc cggaaaccgt gacgtaagag cgtttaccaa tccacatata
    21961 ctgtatgcag aacaccagca gcgagaacag cagcaggaaa gcgccaaggg tgctggcggc
    22021 ctgataatcc agctgcgagc cggtaatata gaagtaaatt tgcgtcgcca gtacgtcgaa
    22081 gttaccgcca agtaccagcg ggttactgaa atcagccagt gactgtacca cgacgattaa
    22141 aaaggcattg gcgagcgcgg gcttcaacaa cggcacaaac acgccgttaa aggtttgcca
    22201 gcggctggcg cgcagggtgt aggaggcctc ttccagcgag ggatgaatgg ttttaatggc
    22261 cccgtcgagg atcataaatg ccattggcgt gaatgccaga acctgtgcca gccagatccc
    22321 ggtaaaaccg tacagccagt tggtgttggt cagcccaaac cactccacca taaattccgt
    22381 aacatagccg gaacgcccca tcatgagcgt cacgcctaag ccgaccacaa acggtggcgt
    22441 aacgatgggc aaaatggaaa agatgcgacc aataatggcg ctgcgtttag cgatgcgcgt
    22501 ggtataaatc gccagcacca ggccgaagaa agcacagcct atccccaccg ctatcgacaa
    22561 gacgatagag ttgacgatca cctgaacaat atgtgcctgc gagagcacgg tcataaacgc
    22621 cagcggggcg agctctcccg cgtcgttggt gaacatcggg ataaaaatgg cgatgctcgg
    22681 ccagacgata aaaatgccaa tcagcgccac aatcgcaatc agtgagccga tcacgaagcg
    22741 gtcgccgccc agccattcga ggcgggttaa ggccagcgtc ataatcgcgc ctagttcgac
    22801 aaacagcacg atggcggcgt agcctaaacc gcgtccagcc acggtggcgc tgatgacgat
    22861 aaacgccata cagagcaatg cccagccggc atcgaaataa tgacgactac gctgttcgcg
    22921 cgtggcctga cgaaacgggc ggaccagcaa caggctcggg agtaaatacc acagccagct
    22981 gatattaaaa tgcgaccagc cgtacgcccc gaggatctca tcactcgtgg attccagcag
    23041 gccataatcg aggctccagc tcggcaacag gacaaatgcc agccagccca gcaaaaccca
    23101 aaggaaaatc gcatcccgct ttttcacggg atggagtgca agtgtctgtg acatagcagt
    23161 tccggtatgg ttggccgggg caggcgtctg cgctgcaccc ggcaaatgcg cctaatgcgg
    23221 gtttatttac ccatcttcac ttcgctgacc catttattaa tcaatgcctt acgaacatcc
    23281 gtggaaccat atttatccat gtcgtagttg attaatttca ggtcgtcgag cttcagcgaa
    23341 tttggcgacg tttcggcggt ggtattagtc aggatctgat aagacttccc tttcttccac
    23401 gccagttcct gagcttcttt tgataatacc cagtccacaa acagtttggc gttgtcgagg
    23461 ttgcgcgcgc ctttcagaat gctgacgccg ccaatttcgt agcccgtacc ttcacacggg
    23521 gaaatcaact ccagcggcgc gccctgttct ttttccagcg agtagtcatg caggaagcca
    23581 ataccaatcg ccgtttcgcc acgggcggcg ttacgtgccg gggctatgcc tgatttggtg
    23641 tactgagaga cgttggcgtt cagttgtttt agataatcaa aggcctgatc ttccccccac
    23701 agctgcgcaa aagtagccag ggcggtgtag gcagtaccgg aactttgcgg atcggcaatt
    23761 tgaatttcgc ctttatattc cggtttggtg agatctttcc agcacttcgg caccggcagg
    23821 tttttctctt tcagacgatc ggtgtttacg ccaaaaccga ggatccccac ataaacggca
    23881 gaggacagat tccctttcac ttttgccgga tcgcggaatt tctccatgat ctgctcaagg
    23941 ttaggggatt tatacgcctg tagcaggccc atctcgccag cctgcgattg cgggtctaaa
    24001 gtgccgccgt accagacgtc agcctggggg tttttctttt cagcatcgac ttttgccagg
    24061 gtgcttccgg aaccgttgcg gataaacgag gtttttacat catatttctc gccaaacgct
    24121 ttggtttcgg cctcacacat ttcattggtg gcgctgcaat agaccaccag ccggcctttt
    24181 gcctgcgcgg cacccgttaa agtcgccagc gcaacggcag aagcaatcag agtagaggta
    24241 aaggtcattt tcattgtttt atccttcgag cgtgagtggt ggtgatgccc gccatcagta
    24301 acggcatcag cagtaaaccc ataagtactg cggcgaccga gagtaaactg aacattcccg
    24361 gccagccgta gcgttcgatg accagcgaca gcggccagcc tgccagcgcc gcgccaaggt
    24421 aagcgaacag gccgagaaat ccggtgattg aacccgccgc agctttgtgc ccacactcca
    24481 ccgccgcaag tccaatcagc atttgtgggc caaagacgaa aaagcccacg gtaaaaaagc
    24541 acactgccag cagagcgtag tgatgaaccg gtgccagcca cagtgcggcg actgacacca
    24601 tcaaccccaa cgtgaacagc aaaatcattg gcgctcgttg cccgctaaac agcaaatccg
    24661 atccccatcc ggcaaataac gcgccgagta agccgcccac ttcaaacagc attaccgtgg
    24721 cgttggcgct gagcaggttg acaccgtggc tttccgtcag ccagatattg ccccagtcgt
    24781 taagcgcaat ccggatcaca taaaccagta catatgagac gcccagtagc cagatcagcg
    24841 gattttgcag catggtggtg cgcaacattt gccacagccc catcggcgga ctttgttgtt
    24901 cctgacgtaa ctccagcgga tcgtgacgcc agtgaccaac tgtcggtaat ccctcttcct
    24961 ggggcgtgcc tttgagttgt agcgttaacc agatccctaa tgccatgctg attatccccg
    25021 gtgtcagcat cgcggcctgc cagccccacc agtgggcggc aaaggcgcta atcagcggaa
    25081 taatcgctcc gccaatgttg atcgacatat tccagcagcc ccaccagaaa ccgcgctcat
    25141 tgcgtgagta ccagtgggtc agcaagcggg cacacggtgg ccatccccag ccctgaaaaa
    25201 aaccgttcag tgtccagacc acaagcagaa gcgtaagtga ttcgccaaag gcaaacacca
    25261 cgttcaataa cccggtggcg aacaagccga cgcccataaa accgcgttgt ccgtggctgt
    25321 catgccacag tccggcggta aatttcgaca atccgtagct gagataaaac agcgaaccca
    25381 gcaggccaat gtcgccttta tccagtccga ggtcggtttg tagcgccgga aggacgtaat
    25441 tcacgctttt tcgcgtcaga taaaatgcgg cgtagccaat aatcatgcac atcagcaatc
    25501 gcggacgtaa tgtccggtag cattgattaa tttctctcgc tgaacgcgcg tgcatggttg
    25561 tctccatgtt gctgactata tgaaaatgaa agtgaaaggg gatgagagaa aatccgggat
    25621 acctaagact ttttcctggt tatccggcga tttgttgcaa aaatgtgggc aagttaacaa
    25681 ttacccgcgt gccgtgctgt ttttcgagcg ttaagtcgcc gcccagggcg ctgacgcgct
    25741 cacgcatccc ctgaatgcca aatccagcca ttttcccgct ggcgatcccc acaccgttgt
    25801 cgctgacttc cagatgcaat acctctccct gctggcgcag gataatcgtc acttcgctgg
    25861 cttcggcgtg tttacagatg ttattgagca actcctgcaa taaccgatac aaggtaaagc
    25921 gcaccgtttc gttttctggt gtggagttca gttgataggc aaactggcaa tgaatgccgc
    25981 gttcactaaa ggcaaattca ttaatcagat gcagcaacgc ttcgcgaaat gtcagttcat
    26041 caagcgcggg aggccgcaac tggcgcagca attggcgggt ggagagatga atgcggcggg
    26101 cgagctcatt gatttggctg gctgcggatt gaatttgcgc cggatcacgc gcccgcttca
    26161 ccagttgcga ctggatctga atggcggtga tgttctgacc gatttcgtcg tgcagctctc
    26221 gcgccaggtt ttttcgcgtg tcctcctcgg tgtggatcag cttttccgcc aatgcccgcc
    26281 gcgctgccag ctcggcctcc agccgttgtc ggtaatggtg caggtttagc gccagatgtt
    26341 gctggcgact gatggcaatg cccaggccaa ttcccagcaa ggcctgggtc gcgaggaaaa
    26401 tctccagctc caccaggtta ctaaagccca cgccaatctg gcgggcgatg gtgatcatca
    26461 tgctgcccaa aagcccggag agtacgccac cttgccagcc aaatttccac gccatcacca
    26521 cattgggcaa aaagaccacg atcaacagca gacgttcaat ttcaggtgac agcaccatct
    26581 gagtgccgat accaataata aaaaacaggc tacaccagat gattaaagag gtacgcaagg
    26641 ggggattatg cgtatcgagt cccaggagct ggtagcgatg ctgctggcgc agaaactcaa
    26701 agataagata gacaaacggc gtcagtaata cgccgccggt aaatgaggtc aaacccggta
    26761 gcattattgg gcctgacata aaaggggaga gcagggcggt attcaacaat gaagctgccg
    26821 tgacagtggc taacagcaac gtcagacgct gccagtaaag tggaaaacgg tgccagaagt
    26881 tttgcgcaag gagggcagga aacaggctaa taaccggcgt tgccagcatg agataaccgt
    26941 tcaatagctg ttcactatgg agccagaaca tcatcagcaa aggggggagc accaacgcag
    27001 gccagtagcg gcgagagagc aaaataacaa gggcgagata aactccctgc gggagaaaca
    27061 acaccgcctg ttgaccgtta ttggtcagat aaaagctgag cgtccacagc atcagccagc
    27121 cggttcccca ggctagcaca ataaacagca aaatcaccca atgacgaaga ttgcgcgaca
    27181 ttaatgcccc gccagcagat ggtggtcgag cgcaaaatgt accagttcaa tggtgctatg
    27241 gcagttcagt tttcccagca cattggcgcg atgcacatgt accgttttat gactgagttc
    27301 gagtttaaag gcgatctctt taacgctgtc tcctttgacc agtaactcaa acacttcacg
    27361 ttctcgtggg gtgagaattt ccagcgcctg ggcgggttgc ccgccgccgc gtaaagcccg
    27421 aatcgcatcg gcacagagat aatgcccgcc cagcccgacc gagcgcaccg cctgtactaa
    27481 ctcttccggc ccacagcgtt tggttaaata cccgctggca ccggcatcca gcgcgctttg
    27541 cacaaaggtt ggcgcgtcat aaatgctaag aataatggcg cgaaattgcg gtttttgtgc
    27601 acgcaggcgc ttaagcaaac tgaggccgtt ttcgtccggc atggcaatat caatgacggc
    27661 aacattgacg tcatcacgga ttaatgccga ccatgcctgc gcggcactac tgtactgacc
    27721 aatgacctcc agatcgtctt ccagagagag taattgtgcg aagccagagc gcaccacaac
    27781 atggtcatcc accagcacta cccgaatcat acttttcttc tcttattatc taattctcct
    27841 atcatgcatc acggcgcggc gctggcaatc aaccagcgac agatatggaa tggatgtaac
    27901 aaaatggcaa taatttgccg gagcgcgcac atcttatctg gcttgtgaac cggcgggata
    27961 cgttcgccaa aaccgcaaaa tgcgcaaact cgctaaccat tgccgatttt tttctaaaaa
    28021 cgctttgttc ttaacaagac cttttaattc ctttatcaaa ctgacccgca acgaaatact
    28081 gctcccataa caagacaggg gagcagacaa tcatggcaat ttcatcgcgt aacactcttc
    28141 ttgccgcact ggcattcatc gcttttcagg cgcaggcggt gaacgtcacc gtggcgtatc
    28201 aaacctcagc cgaaccggcg aaagtggctc aggccgataa cacctttgct aaagaaagtg
    28261 gagcaactgt ggactggcgc aagtttgaca gcggagccag catcgtgcgg gcgctggctt
    28321 ctggcgacgt gcaaatcggc aaccttggtt ccagcccgtt agcggttgca gccagccaac
    28381 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
    28441 taaagaaaac tatcagcaaa ccggaagatc tgattggcaa acgcatcgcc gtaccgttta
    28501 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctggggcatt aaacccgggc
    28561 aagtggagat tgtgaacctg cagccgcccg cgattatcgc tgcctggcag cggggagata
    28621 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgt
    28681 tgaccgattc tgaacaggtc gggcagtggg gcgcgccaac gctggacgtt tgggtggtgc
    28741 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttctctaaa agcgccatcg
    28801 atgctcagca accgtacatt gctaacccag acgtgtggct gaaacagccg gaaaacatca
    28861 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggga
    28921 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcga
    28981 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
    29041 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
    29101 ctcatcttta cgccgattat ggcggcaaac cggtactgga agatatcaac ctgacgctgg
    29161 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
    29221 atttgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
    29281 ttgaggggcc gggagcagag cgtggcgtag tttttcagaa tgaagggcta ctaccgtggc
    29341 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
    29401 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
    29461 acatctggca gctttccggt ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
    29521 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
    29581 accagatgca aaccctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
    29641 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcacccg
    29701 gccctggccg cgtgctggag cggctgccgc tcaactttgc tcgccgcttt gttgcgggag
    29761 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
    29821 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
    29881 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
    29941 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
    30001 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
    30061 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
    30121 gctactcgcg gcggtgttga tcggcattcc ggtcgggatc gccatggggc taagcccgac
    30181 ggtgcgcggc attctggatc cgataatcga gctttatcgt ccggtgccgc cgctggctta
    30241 tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct tactgatcta
    30301 tttagcgatt tttgcaccgg tggcgatgtc ggcgctggcg ggggtgaaaa gcgcgcagca
    30361 ggttcgcatt cgtgctgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
    30421 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgtattggtc tgggtgtggg
    30481 ctggtctacg ctggtggcgg cggagctgat tgccgcgacg cgcggtttag gatttatggt
    30541 tcagtcagcg ggtgaatttc tcgcgactga cgtggtgctg gcggggatcg cggtgattgc
    30601 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgcc tgacgccctg
    30661 gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc gtatatcgga
    30721 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacaactt
    30781 taccatgcgg tactgcgcca tcaggtggtg tttctgcgcg atcaagccat tacgccgcag
    30841 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
    30901 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccagataac
    30961 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc gattctggca
    31021 gcgaaagagt taccttcgac cggcggcgat acgctctgga ccagcggtat tgcggcctat
    31081 gaggcgctct ctgttccctt ccgccagctg ctgagcgggc tgcgtgcgga gcatgatttt
    31141 cgtaaatcgt tcccggaata caaataccgc aaaaccgaag aggaacatca acgctggcgc
    31201 gaggcggttg cgaaaaatcc gccgttgcta catccggtgg tgcgaacgca tccggtgagc
    31261 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgttga tgtcagcgag
    31321 aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc ggagtttcag
    31381 gtgcgctggc gctggcaacc gaatgatatt gcgatttggg ataaccgtgt gactcagcac
    31441 tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac gattcttggg
    31501 gataaaccgt tttatcgggc ggggtaatac gagagtggac ggtcctctcg cccccttggg
    31561 gagagggtta gggtgagggg gcgttcaccg tgctttcaac aggttaactc cccctttctg
    31621 agaggaaaca aaattaacgc agaatcttct tctcagccaa atccagcgca aagtagctga
    31681 aaatcagatc cgcacccgca cgcttaatcg aacctaagct ttcgagcacg actttctctt
    31741 catctatagc acccgccagc gcggcgaact taatcatcgc atactcaccg ctcacctgat
    31801 acgcgccaat cggcaattca gtacgttcac gcagctcacg cacgatgtcg aggtacgctc
    31861 cagcaggttt aaccatcagg cagtctgcgc cctgggcttc atccagcagt gactcacgaa
    31921 tcgcctcacg acggttcatt gggttcatct gatagctttt gcggtcgcct tttaatgcgc
    31981 ttccggcagc ttcacggaac ggaccataaa aggaggaggc gaacttggtc gaatacgaca
    32041 taatcgccgt atctttaaag ccagcagcgt ccagcgcctg gcgaatcgcc tgtacctggc
    32101 catccatcgc ggcggaaggg gcgatgaagt ctgcacctgc agcagctgca accacggctt
    32161 gcttgcctaa attttccaga gtcgcgtcgt tgtcgacgcc atgctcgcac agcacaccgc
    32221 agtgaccgtg agaagtgtat tcacagaagc aggtgtctga catgacaatc atttctggca
    32281 ccgtctgctt gcagatgcgc gacatacgcg ctaccagtcc atcttcccgc caggcatcgc
    32341 tgccggtttc atcggtatgg tgagagatgc cgaaagtcat cacggaacga ataccggcgt
    32401 tggcgatgcg ttcaatttcg cgtgccagat gtttctctgg aatgcgcatc acgccaggca
    32461 tggcttcaac ggctttgtag tcgtcaattt cttcttcaac aaagatcggc aacaccaggt
    32521 cgttaaggct aagtgttgtc tcttcaaaca tagcgcgcag cgcaggagat ttgcgcaggc
    32581 gacgagggcg ttggattaag tctgtcatgg tctgcctgat gtttgtggaa ttaaggtgca
    32641 cagtatacct gaagcggggc tggagtgttt tacgaaagtt gtcacgatgt tgacaagaag
    32701 agaatgaaag agagaccagg gcatctttgt aata
//
LOCUS       DAZFKY010000033        32672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFKY010000033 DAZFKY010000000
VERSION     DAZFKY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..890
                     /gene="nanR"
                     /locus_tag="ACHKCL_001393"
     CDS             99..890
                     /gene="nanR"
                     /locus_tag="ACHKCL_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5762394.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1012..1905
                     /gene="nanA"
                     /locus_tag="ACHKCL_001394"
     CDS             1012..1905
                     /gene="nanA"
                     /locus_tag="ACHKCL_001394"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5762395.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2014..3504
                     /gene="nanT"
                     /locus_tag="ACHKCL_001395"
     CDS             2014..3504
                     /gene="nanT"
                     /locus_tag="ACHKCL_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5762396.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3552..4241
                     /gene="nanE"
                     /locus_tag="ACHKCL_001396"
     CDS             3552..4241
                     /gene="nanE"
                     /locus_tag="ACHKCL_001396"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5762397.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4238..5113
                     /gene="nanK"
                     /locus_tag="ACHKCL_001397"
     CDS             4238..5113
                     /gene="nanK"
                     /locus_tag="ACHKCL_001397"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5762398.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5110..5574
                     /gene="nanQ"
                     /locus_tag="ACHKCL_001398"
     CDS             5110..5574
                     /gene="nanQ"
                     /locus_tag="ACHKCL_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5762399.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5634..6683)
                     /gene="yhcG"
                     /locus_tag="ACHKCL_001399"
     CDS             complement(5634..6683)
                     /gene="yhcG"
                     /locus_tag="ACHKCL_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO5762400.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(6867..8285)
                     /gene="gltD"
                     /locus_tag="ACHKCL_001400"
     CDS             complement(6867..8285)
                     /gene="gltD"
                     /locus_tag="ACHKCL_001400"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5762401.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(8298..12758)
                     /gene="gltB"
                     /locus_tag="ACHKCL_001401"
     CDS             complement(8298..12758)
                     /gene="gltB"
                     /locus_tag="ACHKCL_001401"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5762402.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            13432..14361
                     /gene="yhcC"
                     /locus_tag="ACHKCL_001402"
     CDS             13432..14361
                     /gene="yhcC"
                     /locus_tag="ACHKCL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5762403.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            14457..16793
                     /gene="arcB"
                     /locus_tag="ACHKCL_001403"
     CDS             14457..16793
                     /gene="arcB"
                     /locus_tag="ACHKCL_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5762404.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            17023..17676
                     /gene="elbB"
                     /locus_tag="ACHKCL_001404"
     CDS             17023..17676
                     /gene="elbB"
                     /locus_tag="ACHKCL_001404"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5762405.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            17673..18401
                     /gene="mtgA"
                     /locus_tag="ACHKCL_001405"
     CDS             17673..18401
                     /gene="mtgA"
                     /locus_tag="ACHKCL_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5762406.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(18398..19030)
                     /gene="yrbL"
                     /locus_tag="ACHKCL_001406"
     CDS             complement(18398..19030)
                     /gene="yrbL"
                     /locus_tag="ACHKCL_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5762407.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(19244..19516)
                     /gene="npr"
                     /locus_tag="ACHKCL_001407"
     CDS             complement(19244..19516)
                     /gene="npr"
                     /locus_tag="ACHKCL_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5762408.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(19513..20367)
                     /gene="rapZ"
                     /locus_tag="ACHKCL_001408"
     CDS             complement(19513..20367)
                     /gene="rapZ"
                     /locus_tag="ACHKCL_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5762409.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(20413..20904)
                     /gene="ptsN"
                     /locus_tag="ACHKCL_001409"
     CDS             complement(20413..20904)
                     /gene="ptsN"
                     /locus_tag="ACHKCL_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5762410.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            complement(21022..21309)
                     /gene="hpf"
                     /locus_tag="ACHKCL_001410"
     CDS             complement(21022..21309)
                     /gene="hpf"
                     /locus_tag="ACHKCL_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5762411.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(21332..22765)
                     /gene="rpoN"
                     /locus_tag="ACHKCL_001411"
     CDS             complement(21332..22765)
                     /gene="rpoN"
                     /locus_tag="ACHKCL_001411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5762412.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(22813..23538)
                     /gene="lptB"
                     /locus_tag="ACHKCL_001412"
     CDS             complement(22813..23538)
                     /gene="lptB"
                     /locus_tag="ACHKCL_001412"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5762413.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(23545..24102)
                     /gene="lptA"
                     /locus_tag="ACHKCL_001413"
     CDS             complement(23545..24102)
                     /gene="lptA"
                     /locus_tag="ACHKCL_001413"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5762414.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(24071..24646)
                     /gene="lptC"
                     /locus_tag="ACHKCL_001414"
     CDS             complement(24071..24646)
                     /gene="lptC"
                     /locus_tag="ACHKCL_001414"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5762415.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(24643..25209)
                     /gene="kdsC"
                     /locus_tag="ACHKCL_001415"
     CDS             complement(24643..25209)
                     /gene="kdsC"
                     /locus_tag="ACHKCL_001415"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5762416.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(25230..26216)
                     /gene="kdsD"
                     /locus_tag="ACHKCL_001416"
     CDS             complement(25230..26216)
                     /gene="kdsD"
                     /locus_tag="ACHKCL_001416"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5762417.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(26230..27207)
                     /gene="yrbG"
                     /locus_tag="ACHKCL_001417"
     CDS             complement(26230..27207)
                     /gene="yrbG"
                     /locus_tag="ACHKCL_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5762418.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            27417..28226
                     /gene="mlaF"
                     /locus_tag="ACHKCL_001418"
     CDS             27417..28226
                     /gene="mlaF"
                     /locus_tag="ACHKCL_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5762419.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            28234..29016
                     /gene="mlaE"
                     /locus_tag="ACHKCL_001419"
     CDS             28234..29016
                     /gene="mlaE"
                     /locus_tag="ACHKCL_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5762420.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            29021..29572
                     /gene="mlaD"
                     /locus_tag="ACHKCL_001420"
     CDS             29021..29572
                     /gene="mlaD"
                     /locus_tag="ACHKCL_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5762421.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            29591..30226
                     /gene="mlaC"
                     /locus_tag="ACHKCL_001421"
     CDS             29591..30226
                     /gene="mlaC"
                     /locus_tag="ACHKCL_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5762422.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            30226..30519
                     /gene="mlaB"
                     /locus_tag="ACHKCL_001422"
     CDS             30226..30519
                     /gene="mlaB"
                     /locus_tag="ACHKCL_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5762423.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            30679..30933
                     /gene="ibaG"
                     /locus_tag="ACHKCL_001423"
     CDS             30679..30933
                     /gene="ibaG"
                     /locus_tag="ACHKCL_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5762424.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            30988..32247
                     /gene="murA"
                     /locus_tag="ACHKCL_001424"
     CDS             30988..32247
                     /gene="murA"
                     /locus_tag="ACHKCL_001424"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5762425.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(32295..32573)
                     /gene="sfsB"
                     /locus_tag="ACHKCL_001425"
     CDS             complement(32295..32573)
                     /gene="sfsB"
                     /locus_tag="ACHKCL_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5762426.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         7506 a         8336 c         8829 g         8001 t
ORIGIN      
        1 taatgatgag gcacaaagag aaaaccctgc cattttcccc tactttcaat cctgtgatag
       61 gatgtcactg atgatgttaa tcacactgac cttacagaat gggccttatg aacgcatttg
      121 attcgcaaac cgaagattct tcacctgcaa ttggtcgcaa cttgcgtagc cgcccgctgg
      181 cgcgtaaaaa actctccgaa atggtggaag aagagctgga acagatgatc cgccgtcgtg
      241 aatttggcga aggtgaacaa ttaccgtctg aacgcgaact gatggcgttc tttaacgtcg
      301 ggcgtccttc ggtgcgtgaa gcgctggcag cgttaaaacg caaaggtctg gtgcaaataa
      361 acaacggcga acgtgcccgc gtctcacgtc cttctgcgga caccatcatc ggcgagcttt
      421 ccggcatggc gaaagatttc ctttctcatc ctggtgggat tgcccatttc gaacaattac
      481 gtctgttctt tgaatccagc ctggtgcgct atgcggctga acacgccacc gatgagcaaa
      541 tcgatttgct ggcaaaagca ctggaaatca acagtcagtc gctggataac aacgcggcat
      601 tcattcgctc agacgttgat ttccaccgcg tgctggcgga gatccccggt aacccaatct
      661 tcatggcgat ccacgttgcc ctgctcgact ggctgatcgc cgcacgtcca accgtggctg
      721 atcaggctct gcacgagcat aacaacgtta gctatcaaca gcatattgcg atcgttgacg
      781 cgatccgccg ccacgatcct gacgaagccg atcgtgcgtt gcaatcgcat ctcaacagcg
      841 tctctgctac ctggcacgcc ttcggtcaga ccaccaacaa aaagaaataa tgccacttta
      901 gtgaagcaga tcgcattata agctttctgt atggggtgtt gcttaattga tctggtataa
      961 caggtataaa ggtatatcgt ttatcagaca agcatcactt cagaggtatt tatggcaacg
     1021 aatttacgtg gcgtaatggc tgcactcctg actccttttg atcaacaaca agcactggat
     1081 aaagcgagtc tgcgccgcct ggttcagttc aatattcagc agggcattga cggtttatac
     1141 gtgggtggtt cgaccggcga agcctttgta caaagccttt ccgagcgtga acaggtactg
     1201 gaaatcgtcg ccgaagaggc gaaaggtaag attaaactca tcgcccacgt cggttgcgtc
     1261 agcaccgccg aaagccaaca acttgcggca tcggctaaac gttatggctt cgatgccgtc
     1321 tccgccgtca cgccgttcta ctatcctttc agctttgaag aacactgcga tcactatcgg
     1381 gcaattattg attcggcgga tggtttgccg atggtggtgt acaacattcc agccctgagt
     1441 ggggtaaaac tgaccctgga tcagatcaac acacttgtta cattgcctgg cgtaggtgcg
     1501 ctgaaacaga cctctggcga tctctatcag atggagcaga tccgtcgtga acatcctgat
     1561 ctggtgctct ataacggtta cgacgaaatc ttcgcctctg gcctgctggc gggcgctgat
     1621 ggtggtatcg gtagtaccta caacatcatg ggctggcgct atcaggggat cgttaaggcg
     1681 ctgaaagaag gcgatatcca gaccgcgcag aaactgcaaa ctgaatgcaa caaagtcatt
     1741 gatttactga tcaaaacggg tgtattccgc ggcctgaaaa ctgttctgca ttatatggat
     1801 gtcgtttctg tgccgctgtg ccgcaaaccg tttggtccgg tagatgaaaa atatctgcca
     1861 gaactgaagg cgctggccca gcagttgatg caagagcgcg ggtgagttgt ttcccctcgc
     1921 tcgcccctac cgggtgaggg gaaataaacg catctgtacc ctacaatttt cataccaaag
     1981 cgtgtgggca tcgcccaccg cgggagactc acaatgagta ctacaaccca gaatatcccg
     2041 tggtatcgcc atctcaatcg tgcacaatgg cgggcatttt ccgctgcctg gttgggatat
     2101 ctgcttgacg gttttgattt cgttttaatc gccctggtac tcaccgaagt acaaggtgaa
     2161 ttcgggctga cgacggtgca ggcggcaagt ctgatctccg cagcctttat ctctcgctgg
     2221 ttcggcggcc tgatgctcgg cgctatgggt gaccgctacg ggcgtcgtct ggcaatggtc
     2281 accagcatcg ttctcttctc ggccgggacg ctggcatgcg gctttgcgcc aggctacatc
     2341 accatgttta tcgctcgtct ggtcatcggc atggggatgg cgggtgaata cggttccagc
     2401 gccacctatg tcattgaaag ctggccaaaa catctgcgta acaaagccag tggctttctg
     2461 atttcaggct tctctgtagg ggccgtcgtt gccgctcagg tctacagcct ggtggttccg
     2521 gtctggggct ggcgtgcgct gttctttatc ggcattttgc caatcatctt tgctctctgg
     2581 ctgcgtaaaa acatcccgga agcagaagac tggaaagaga aacacgcagg taaagcacca
     2641 gtacgcacaa tggtggatat tctctaccgt ggtgaacatc gcattgccaa tatcgtaatg
     2701 acactggcgg cggctactgc gctgtggttc tgcttcgccg gtaacctgca aaatgccgcg
     2761 atcgtcgctg ttcttgggct gttatgcacc gcaatcttta tcagctttat ggtgcagagt
     2821 acgggcaaac gctggccaac tggcgtaatg ctgatggtgg tcgtgttgtt tgctttcctc
     2881 tactcatggc cgattcaggc gctgctgcca acgtatctga aaaccgatct ggcttataac
     2941 cctcatactg tcgccaatgt gctgttcttt agtggctttg gcgcggcggt ggggtgctgc
     3001 gtcggtggct tcctcggtga ctggctggga acccgcaaag cgtacgtttg tagtctgttg
     3061 gcctcgcagc tgctgattat tccggtattt gctattggcg gtgcaaacgt ctgggtgctc
     3121 ggtctgttac tgttcttcca gcaaatgctt ggacaaggga tcgccgggat cttaccgaaa
     3181 ctgattggcg gttatttcga taccgaccag cgtgcagcgg gcctgggctt tacctataac
     3241 gttggcgcat tgggtggcgc actggcccca atcatcggcg cgttgatcgc tcaacgtctg
     3301 gatctgggta ctgcactggc atcgctctcg ttcagtctga catttgtggt gatcctgctg
     3361 attggtctgg atatgccttc tcgtgttcag cgttggctgc gcccggaagc gttgcgtact
     3421 catgacgcta tcgacggcaa accatttagc ggtgccgtgc cgtttggcag cgccaaaaac
     3481 gatttagtca aaaccaaaag ttaatcctgt tgcccggtct ctgtatcggg cctttcgcta
     3541 agggaagatg tatgtcgtta cttgcacaac tggatcaaaa aatcgctgct aacggtggcc
     3601 tgattgtctc ctgccagccg gttccggaca gcccgctcga taaacccgaa atcgtcgcag
     3661 ccatggcatt agcggcagag caggcgggcg cggttgccat tcgcatcgaa ggtgtggcaa
     3721 atctgcaagc cacgcgtgcg gtggtgagcg tgccgattat tggaattgtg aaacgcgatc
     3781 tggaggattc tccggtacgc atcacggcct atattgaaga tgtcgatgcg ctggcgcagg
     3841 cgggcgcgga cattatcgcc attgacggca ccgaccgccc gcgtccggtg cctgttgaaa
     3901 cgctgctggc acgtattcat catcacggtt tactggcgat gaccgactgc tcaacgccgg
     3961 aagacggcct ggcatgccaa aagctgggag ccgaaattat tggcactacg ctttctggct
     4021 ataccacgcc tgaaacgcca gaagagccgg atctgacgct ggtgaaaagg ttgagcgacg
     4081 ccggatgtcg ggtgattgcc gaagggcgtt acaacacgcc tgctcaggcg gctgatgcga
     4141 tgcgccacgg cgcgtgggcg gtgacggtcg gttctgccat tacgcgtctt gaacacattt
     4201 gtcagtggta caacacagcg atgaaaaagg cggtgctatg accacactgg cgattgatat
     4261 cggcggtact aaacttgccg ccgcgctgat tggcgctgac gggcagatcc gcgatcgtcg
     4321 tgaacttcct acgccagcca gccagacacc agaagccttg cgtgatgcct tatccgcatt
     4381 agtctctccg ttgcaagctc atgcgcagcg ggttgccatc gcttcaaccg gaattattcg
     4441 cgacggcagt ttgctggcgc tgaatccgca taatcttggt ggattgctac actttccgtt
     4501 agtcaaaacg ctggggcaac ttaccgattt gccgaccatt gccattaacg acgcccaggc
     4561 cgcagcatgg gcagagtatc aggcgctgga aggcgatata accgatatgg tgtttatcac
     4621 cgtttccact ggcgtcggcg gcggtgtagt gagcggcggc aaactgctta ccggccctgg
     4681 cggtctggcg gggcacatcg ggcatacgct tgccgatcca cacggcccag tctgcggctg
     4741 tggacgcaca ggttgcgtgg aagcgattgc ctctggtcgc ggtattgcag cggcagcgca
     4801 gggggagttg gctggcgcgg atgcgaaaac tattttcatg cgcgccgggc agggcgacga
     4861 gcaggcgcag cagctgattc accgctccgc acatgtgctg gcaaggctga tcgctgatat
     4921 taaagccaca actgattgtc agtgcgtggt ggtcggtggc agcgttggtc tggcagaagg
     4981 gtatctggcg ctggtggaaa cgtatctggc gcaggagcca gcggcatttc atgttgattt
     5041 actggcggcg cactaccgcc atgatgcagg tttacttggg gctgcgctgt tggcccaggg
     5101 agaaaaatta tgatgatggg tgaagtacag tcattaccgt ctgctgggtt acatcctgcg
     5161 ttacaggacg cgttaacgct ggcattagct gccagaccgc aagaaaaagc gccgggtcgt
     5221 tacgaattac agggcgacaa tatttttatg aatgtcatga cgtttaacac tcaatcgccc
     5281 gtcgagaaaa aagcggaatt gcacgagcaa tacattgata tccagctgtt attaaaaggt
     5341 gaggaacgga ttctgtttgg cacggcaggc actgcgcgcc agtgtgaaga gttccaccat
     5401 gaagatgatt accagctttg cagcgccatt gaaaacgagc aaaccatcaa cttaaaaccg
     5461 ggaatgttcg ccgtgtttat gccgggagaa ccgcataaac caggatgcgt tgtcggcgaa
     5521 cctggtgaga ttaaaaaggt ggtggtgaag gttaaggctg atttaatggc ttaaaactta
     5581 gttcgtttta aagattctgc agacagtgtc tgcagaattc gaaaccgctg aaattaacgg
     5641 agtctatagc cttcttctag caccgcctgt gttcgaacga gttcatccgc gagtcgtttt
     5701 tcatcaggta gttgcatctt atattcactc gccagaacgg tgttaggcaa accagccagt
     5761 gcataatgcg cttctccgac tcctttctct gcacagagaa ccagaccgat gggcggattt
     5821 tcatccggta gcgtccagtg ctctttggcg tagttgagat acatattcat ctgtccggca
     5881 tcgctatagc tgaatttgcc cacttttaga tcgacgatta gcaggcagcg taaacggcgg
     5941 tggaaaaaca gcagatcgac acgaaaccag ttgtcatcaa tgcgtaacct tcgctgccga
     6001 ccaacaaagg caaaatcatc cccaagttcc agcatgaaat ccatcaggtg gttgatcagc
     6061 gcctcctcaa aatcggattc tgagtattca tctttcaatt ccagaaattc gagcacaaag
     6121 ggatcgcgta tcgcctgttg cggaagaata tgcgtctcgg caggagcgtg ttgttgcagc
     6181 atggctgatt tgtcatgtga cagcagtgtt cgctcataaa attgggtcgc aatttgccgt
     6241 tctagctggc gaacagacca gccacagcgg agcgtctctt tttcataaaa gctgcgggtg
     6301 tcagcgtttt taacggaaag taaacggacg taagtggacc agggcaatgg tgttaattcg
     6361 ccagacactg tctggcgaat ttcaacatgt tgaaaaaaga ggtaaaaaag cctcatttgg
     6421 cgtaagtttt ttgcagagaa cccacgctta taccttagac ttaaatcctt tgatagtcgc
     6481 ttgattagct gcgcaccata cgcagccctg gcctcgccac cttgttcaaa ttcgacaatt
     6541 cgtcggccaa tttcccagta tgtcgcggtc atcagcgcgt taacgctacg taccgtttcc
     6601 gtccgggcgc tatcgaccag gtgaataata ccgtcgtgga tttgctggta gcctgttgag
     6661 gttctctctg agagcgattc cattatcttt ccctttatat ggcgcagtgt tgaataatgg
     6721 aatcagctat acgccgggca tctgccggta acaatccaga tgtgcgtatt acattggttt
     6781 tcttcagatt atgcgaggcg ctttccagaa cgaaaggaga atttcagcgc cgactctttt
     6841 ctgaggaggc ggcgctggga cagagcttaa acttccagcc agttcataat gccgtctgcc
     6901 gccttacgac cttcggcaat agcggtaacg accagatcgg aaccacggac gatatcgccg
     6961 ccagcaaaga ttttcgggtt gctggtctgg aaagcgttgt cgctgccttc cggggcgatg
     7021 atgcggcctt gcgaatccag ctcgacgctg tgttttgcca gccattccat gctgtgtgga
     7081 cggaaaccaa acgccatgat caccgcatct gctggaacga tatgttcgga acctgccacg
     7141 atctccgcgc ggcgacggcc tttggcatcc ggttcgccca tttcagtacg caccattttt
     7201 acgccgctga ctttgccgtt accgttcact tcaattccca gtggctggac attgaatttg
     7261 aactctacac cttcttcccg cgcgtttttc acttcgcggc gggaacccgg catgttctct
     7321 tcatcacgac gataggcaca ggtaacgtgc tttgccccct ggcgtacaga cgtgcgcacg
     7381 cagtccattg cggtatcgcc accgccaagt actaccacgc gtttgccttc catgctgacg
     7441 aacggttctt cacgagtttc gccaaagccc attaactgtt tggtgttggc gataaggaac
     7501 ggcagcgctg cgtacacgcc atcggcgtct tcgttttcca gcccgccgcg cattgactga
     7561 taagtcccga cgccaaggaa cacggcatcg taatcactca gcagatcgtc cagctgtacg
     7621 tcgcggccca cttcggtatt gagtttgaat tcaataccca tgccggtgaa gatttcacgg
     7681 cggcgcgtca ttacctcttt ttccagtttg aaagccggaa taccgaaggt cagcaagccg
     7741 ccaatttccg ggtgacggtc gaagacgacc gcttgtacgc cgttgcgcgt caggacatct
     7801 gcacacgcca gacctgccgg acctgcgccg ataatcgcca cttttttacc ggtctgtttc
     7861 acgccggaca tatccggacg ccagcccatt tcgaacgctt tatcattgat ataacgctca
     7921 atgttgccga tggtcaccgc gccaaactca tcgttcaggg tgcaggaacc ttcgcacaga
     7981 cggtcttgcg ggcagacacg tccgcatact tccggcagag tgttggtctg gtgcgacagt
     8041 tctgccgctt caaaaatacg cccctcgttg gcgagcttca gccagttcgg gatgtagttg
     8101 tgtaccgggc atttccactc gcagtatggg ttgccgcacg acaggcagcg gtcagcctgc
     8161 gctttggcct ggccttcgga aaacggctcg taaatttcaa caaactcaat tttgcggatc
     8221 ttcagcggtt tctttggcgg atcaacgcgc tgcaggtcga taaattgata aacattctga
     8281 ctcattgttg ctacccctta ctgcgcctgc acgcgcaact ctgctgcgct acgactacga
     8341 tgacccagca gtgctttgac atcactggac ttcggtttaa ccagcgcaaa tttagtggcg
     8401 aaggttgacc agttcgccag aatctcttca ccacgctggg aaccggtatg ctgcacatgc
     8461 tcggtgataa gaccacgcag atgctcttca tggatcgcca aatcgtcaac gcttaatact
     8521 tccaccagtt ccgggttaac gcgtttgcgg aaatcgccgc tttcatcgag aacgtaagcg
     8581 aagccgccgg tcatacccgc accgaagtta acgccggttt tacccagaat acagacgata
     8641 ccgcccgtca tatattcaca gccgttgtcg ccaatgcctt ctaccacggt gattgcaccg
     8701 gagttacgca cgccgaaacg ttcacccgcg cggcctgcgg catacagacg accacccgtc
     8761 gctccataca ggcaggtgtt gccgataata cttgcttcat ggctgcggaa ggcggaacca
     8821 accggaggac gaatggcgat taagccgccc gccatgcctt taccgacata gtcgttggca
     8881 tcaccggtca ggtacagttc cacgccgccc gcgttccaca cgccgaagct ctggcctgcg
     8941 gtgccgttga agtacgcttt aatcggatcg gctgccagcc cctgatcgcc gtgcgtctgg
     9001 gcgatatagc ctgaaagcga cgcgccgaca gagcggtcgg tgttgcggat atcgaaccag
     9061 aaggttttgc tctggcgctc atcgacaaac ggtttcgcct gttgcagcaa ctgcgcgttc
     9121 agcaggccgt tatcaaacgg cgggttggtt tcggtgcagt agagtgcctt acctggatgc
     9181 ggttcggcgg tctccagcag cttcgacagc gccagtttct gctgtttggc ggtgaaaccg
     9241 tccagctctt tcagcaggtc ggtgcgacca atcagatcca ccagacgtgt tacgccaagc
     9301 tgtgccatca gctcgcgggt ttcacgggcg ataaactcaa agtaattcgt caccttgaac
     9361 ggcaggccgt gatagtgatt cttacgcagt ttgtcatcct gagttgccac acccgttgcg
     9421 cagttgttca ggtggcaaat acgcagatat ttacagccga gcgcgaccat tgggccagtg
     9481 ccgaagccga agctttctgc gccgagaatc gccgctttga tgatatcgac acccgttttc
     9541 aggccgccat cgacctgcaa acggatttta tgacgcaagc cgttggcaac cagcgcctgt
     9601 tgtgtttcaa caagtcccag ctcccacgga cagcctgcgt atttcaccga tgaaagcgga
     9661 cttgcgccgg tgccgccgtc atagcctgcg atggtgatta aatcggcata agctttcgcc
     9721 acaccggtgg cgatggtgcc aacgcccggt tcggaaacca gcttcacgga gatcatcgct
     9781 ttcgggttaa cctgcttgag gtcgaaaatg agctgcgcta agtcttcgat agagtagata
     9841 tcgtggtgcg gcggcgggga gatcagcgtc acgcccggca ccgaatagcg cagtttggca
     9901 atgtaaggag tgactttgtc gcccggcaac tgaccgcctt cgcccggctt cgcgccctgg
     9961 gcgactttaa tctgaatgac gtcggcattg accagatacg ccggagtaac cccaaagcga
    10021 ccggaagcca cctgcttgat gcgcgacact ttgttggtgc cgtagcgcgc cgggtcttca
    10081 ccaccttcac cggagttcga gtttccgccg atgctgttca tcgcttccgc cagcgcctcg
    10141 tgggcttccg ggcttaacgc gccgatagac atcgccgcgg tatcgaagcg tttaaacagt
    10201 tcgcttgccg gttcaacatc ggcaatgttg accgcgtttt cacccggcgt aattgccagc
    10261 agatcgcgca gcgtggttgc cggacgctca ttaaccagct tcgcgtattc ctgatagtcg
    10321 ctatactcgc cgctctgtac cgcctgttgc agcgtgcgca ccacgtccgg gttgtaggcg
    10381 tggtattcgc cgccgtggac gtatttcagc aggccgccct ggctgatggg cttgcgcgcc
    10441 agccaggctc gtttcgagag attcagcaga tcctgctgga agtcttcaaa gctcgccccc
    10501 ccaatgcggc tgaccgcccc ctggaagcac aggcccacta catcattgtg cagaccgacc
    10561 gcttcaaaca gtttcgagca gcggtaagag gcgatggtgg agatgcccat tttggacatg
    10621 attttgtaca agcctttgtt gatgccgtta cggtagttga gcatcacggt acgataatct
    10681 ttggcaatcg cgtgggtgtc taccaggcgg cccagcgttt cataggcaag gtacggataa
    10741 atagccgtcg cgccgaaacc cagcaacacg gcgaagtggt gcggatcgcg ggcgctggcg
    10801 gtttcgacga tgatgttggc atcgcaacgc aggctttgat cgaccagacg ggtctggatc
    10861 gcgccaaccg ccattggagc cggaaccggc aggcgatctt tagcgatatt ccggtcggag
    10921 agcaccagca gcacggtgcc gctacgtacc attttttcgg ctttgtcgca cagctctttg
    10981 accgtcgctt cgagcgtggt tttggtgacg tcaaaggtga tatccagcgt atctgcgcgg
    11041 tagtgctcct ctttcatcgt cgtgagctgt ttgaaatcgg agtagagcag aatcggcgat
    11101 ttaaagctta aacggtgcgc ctggccctct gcttcgcaaa agacgttcat ttcacgaccg
    11161 atactggtgg cgagcgacat aacatgcgct tcacgcagcg ggtcgattgg cgggttagtc
    11221 acctgggcaa actgctggcg gaagtagtcg taaataatgc gcggctgact ggagagcacg
    11281 gcgaatgggg tatcatcgcc catcgaaccg accgcttcct gaccgttttc gcccagtacg
    11341 cgaattacgg agtccagctc ttccgcgctg tagttaaact gtttctggta gctggcaagc
    11401 gtgtcgtcgt ccagttcgcg gctacccact tcttcatcgg gcagatcttc aaacggtacc
    11461 agtcggcgga cgtttttctc catccactct ttataagggt ggcggctttt cagatcgtca
    11521 tcggtttctg ccgagtgcag aatacgccca ctgcgagtgt cgattaccat cagttcgcct
    11581 ggcccgacgc ggcctttttc gaccacttcg tcaggctggt aatcccagat accgacttca
    11641 gaggcgcagg tgatgagctt atctttggtg atgacgtagc gcgccggacg cagaccgtta
    11701 cggtcgaggt tacaggcagc aaaacgaccg tcagacatca cgatgcccgc cgggccatcc
    11761 cacggctcca tatgcatgga gttaaagtca aagaaggcac gcagttccgg gtccatatcc
    11821 gggttgttct gccaggcggg tggtactaat agacgcatgg cgcggatgat atccatcccg
    11881 cctgccagca gcagttccag catgttatcc atcgaactgg agtcagagcc gatttcgttg
    11941 acgaacggtg cggcgtcgtg caggtcaggg ataagcggtg tctggaattt ataagtacgc
    12001 gcacgcgccc attggcggtt accggtgatg gtgttgattt caccgttatg cgccaggtag
    12061 cggaacggtt gcgccagcgg ccagcgcggt acggtgttag tggagaagcg ctggtggaac
    12121 aggcaaatgg ccgattccag acgcaggtcc gcaagatcta gataaaagcg cggcagatcc
    12181 gccggcatac acagaccttt atagatgttc accagattcg acaggctaca gacgtagaag
    12241 tctttgtcgg cttcgagacg cttttcaatg cggcggcggg cgataaacag acggcgctcc
    12301 atatcgcgcg gacgccagcc tgccggggcg ttcacaaaaa tttgctcaat gcgtggcaga
    12361 gaggagaggg cgatttcacc cagcacgcct tcgttagtgg ggacatcacg ccagcccaca
    12421 atcgacaagg tttcgcgttg cagctcttct tcaacgatgc ggcgtgcggc agcggcgagt
    12481 tcaggatctt tattcaggaa gagcatcccg acggcgtagt ttttcgctaa acgccagccg
    12541 cgctcctgcg caacgatgcg aaaaaagcga tccggttttt gtaacagcaa gccgcaaccg
    12601 tcaccggttt taccatcggc gagaatcgcg ccacggtgct gcatgcgggc cagtgcgtgt
    12661 attgcagtac gcactacctt gtggctaggt tcgccttcta tgtgggcgat caggccgaaa
    12721 ccacagttat ccctctcaag ggatttatcg tacaacatat cgtgaacctc ccccaggctc
    12781 tgcgggaacc ccacttccga accgctgcgc acgggcacag aaagagcgtg gcgacggggt
    12841 ttgcgtgtca tacgcgcgcc tcgcattcgc ccccttgttg tccttttcgc atcggttaat
    12901 acggtgaaga ggggatctca attactgcat aaatatgatg agcaggctgc tcatccagaa
    12961 agcttccagc ggatttccaa cttatcggga aagctgatac aggtcaaatg gcaagcttat
    13021 tggtacagaa atgtgcttta gcgcaaaatt ctttattaat tgactgaaat taaaatggat
    13081 tttctaatta aggctaaaag gggcgtcgtt agagaaacag ccaattcctg actgtcataa
    13141 aggcgtgtta acccttatcc tgccttgaac tgacgagaaa accagcattt tatactgcct
    13201 taattggtaa tgacgtttca tgctactgtt ttgcctaaaa tccataagga gataaaaaaa
    13261 tctgacacaa gataagcaaa agtgatttat gtgattatgc atgaaatttc ggtaattttg
    13321 attggttatg cagttgataa aagcaggccg ggacgattga tccaagtcat cgccgaattg
    13381 agctaaaaat ggcaggcttt ggcctctttt caaggcgcgg tctatcagat tatgcagtta
    13441 cagaaattag tcaatatgtt tggtggtgat ctcacccgtc gttatgggca aaaggtgcat
    13501 aagctgacgc tgcacggcgg ttttagctgc cctaaccgtg acggtaccat cgggcgtggc
    13561 ggctgcacat tctgtaatgt tgcctcattt gccgatgaag cgcagcaaca tcgttccatt
    13621 gccgagcaac tggcgcacca ggcgaattta gttaaccgcg ctaaacgcta tctggcctac
    13681 tttcaggcgt ataccagcac ctttgcggaa gttcaggtgc tgcgttctat gtatcagcag
    13741 gcggtgagcc aggccaatat tgtcggtttg tgtgttggta ctcgcccgga ctgcgtgccg
    13801 gatgcggtgc tggatctgct ttgcgaatat aaggaccagg gctacgaagt gtggctggag
    13861 ctggggctac aaaccgccca cgacaaaacc ctgcatcgca ttaatcgcgg ccatgatttt
    13921 gcctgttatc agcgtacaac ccagctggca cgtcagcgcg ggctgaaggt gtgttctcac
    13981 ttaattgtcg gcctgcctgg cgaagggcag gctgaatgtt tgcaaacgct ggagcgagtg
    14041 gttgaaaccg gcgtggacgg cataaagctg catccgctgc atattgtgaa aggcagcatt
    14101 atggcgaaag cctgggaagc ggggcgtttg aacggtattg aactggagga ttacacgctc
    14161 actgccggag aaatgattcg ccatacgcca ccggaagtga tctaccaccg catttccgcc
    14221 agcgcccgtc gcccgacgct gcttgctcct ctgtggtgcg aaaatcgatg gacgggaatg
    14281 gtcgagctgg atcgctatct gaatgaacat ggcgtacagg gatcggcgct gggacgtccg
    14341 tggctacctc caacggagta ggtcgttgag gggaattccg catttctcac acaatttata
    14401 acgtaactgt cagaattggg tattattggg gcaggttgtc gtgaaggaat tccctaatga
    14461 agcaaattcg tctgctggcg cagtattatg ttgacctgat gatgaagtta ggtctggtgc
    14521 gcttctcaat gttgctggcg ctggccctcg tcgttcttgc cattgtggta caaatggcgg
    14581 taaccatggt gctgcatggt caggtcgaaa gcattgatgt tattcgttct atcttctttg
    14641 gtttgctgat tacgccgtgg gcggtctact ttctatcggt ggtcgtcgag caactggagg
    14701 agtcacgaca acgtctgtca cggctggtgc aaaaactgga ggagatgcgc gagcgcgatt
    14761 tgagcctcaa cgttcagtta aaagataata ttgcccagct aaatcaggaa attgccgttc
    14821 gtgaaaaagc ggaagcagaa ctgcaggaaa ccttcggcca actgaaaatt gaaatcaaag
    14881 agcgcgaaga gacacaaatt cagctcgagc agcaatcctc atttttacgt tccttccttg
    14941 atgcttcacc cgacctggtt ttttatcgta acgaagataa agagttttcc ggctgtaacc
    15001 gcgcgatgga gctgctgacc ggaaaaagcg aaaaacaact ggttcacctg aaacctgctg
    15061 atgtttactc accggaagcc gctgcgaaag tcattgaaac cgatgaaaaa gtgttccgtc
    15121 ataatgtgtc actgacctat gagcagtggc tggattatcc cgacggacgc aaagcctgct
    15181 ttgaaattcg taaagtgccg tactacgacc gcgtgggtaa acgtcacggt ttgatgggct
    15241 ttggtcgcga cattaccgag cgtaagcggt atcaggatgc gcttgaacgc gccagccgcg
    15301 acaaaacgac gtttatttcc accatcagcc acgaattgcg tacaccgctg aacggtatcg
    15361 tcggcctgag ccgcattctg ctggataccg aactcaccgc cgagcaggaa aaatatctca
    15421 agaccatcca tgtttcggcc gtcacgctgg ggaatatctt taacgatatt atcgacatgg
    15481 ataagatgga acggcgcaag gtccagcttg ataatcaacc ggttgatttc accagcttcc
    15541 ttgccgatct ggaaaatctc tccgcattgc aggcgcaaca aaaaggattg cgctttaacc
    15601 tggagccgac gctgccatta ccgcatcagg tcattaccga cgggacgcgt ttacggcaga
    15661 tcctgtggaa cctcatcagt aacgccgtca aattcaccca gcaaggccag gttaccgtgc
    15721 gcgtgcgcta cgatgaaggc gatatgctgc attttgaagt ggaagattct ggtatcggca
    15781 ttccgcagga tgagctggat aaaatcttcg ccatgtatta ccaggtgaaa gacagtcatg
    15841 gcggtaaacc tgccaccggc accggtattg gtctggccgt ttctcgtcgt ctggcgaaaa
    15901 atatgggcgg cgatattacg gttaccagcg aacagggtaa aggttcaacc tttacgttga
    15961 cgatccacgc accgtcggta gcagaagagg tcgatgatgc gtttgatgaa gacgatatgc
    16021 ctttaccggc gctgaatgtg ctgctggtgg aagacattga actgaacgtg attgtcgcgc
    16081 gttctgtgct ggaaaaatta ggtaacagcg ttgatgtcgc catgaccggc aaggcggcgc
    16141 tggagatgtt taaaccgggc gaatacgacc tggtgttgct ggatattcag ttgccagata
    16201 tgaccgggct ggatatctct cgtgaactga cgaagcgtta tccgcgagag gatttaccgc
    16261 cgctggtggc cttaaccgct aacgtgctga aagacaaaca agagtacctc aatgctggaa
    16321 tggatgatgt gctgagtaag ccgctttctg ttccggcgct aaccgcgatg atcaagaaat
    16381 tctgggatac ccaggatgat gaggagagta cggtgacgac agaagagaac agtaaatcag
    16441 aagcattgct cgatattccc atgctggaac agtatctcga acttgtagga ccgaagctga
    16501 tcaccgacgg gttagcggtg tttgagaaga tgatgccagg ctatgtcaac gtgctggagt
    16561 cgaatctgac ggcgcaggat aaaaaaggca ttgttgagga aggacataaa attaaaggtg
    16621 cggcggggtc agtggggttg cgccatctgc aacagctggg tcagcaaatt cagtctcctg
    16681 accttcccgc ctgggaagat aacgtcggtg aatggattga agagatgaaa gaagagtggc
    16741 gtcacgacgt agaagtgctg aaagcgtggg tggcaaaagc cactaaaaaa tgaccccggc
    16801 tagaccgggg tgcgcgaata ctgcgccaac accagggaaa tcttggctgc gccgtaaatt
    16861 attatgatga gttacaaggg cactgcactg tttttcaggc cgcacgcagt taagatagca
    16921 aaacttaaat gatttgttac atgaatcagt taaatgtgtg atgcgtacca tttttattaa
    16981 aaaaattaat gtgtttcagt caattgcgaa aggatgatca taatgaagaa aattggcgta
    17041 attctgagcg gatgcggcgt ctatgacggt tctgaaattc atgaagcggt gttgacgttg
    17101 ttagctattt cacgcagcgg tgcgcaggcg gtctgctttg caccggataa gcagcaggtt
    17161 gatgttatca atcatttaac tggcgaagcg atgacggaaa cgcgcaatgt gctgattgaa
    17221 gcggcacgaa taacgcgcgg tgaaatccgt cctctggccc aggccgatgc cgctgaactg
    17281 gatgcgttga ttgtgccggg ggggtttggc gcggcaaaga atttaagcaa ttttgccagt
    17341 cttggtcgcg aatgcaccgt tgaccgtgaa ttaaaggcgc tggcacaagc gatgcatcag
    17401 gccggaaaac cgcttggttt tatgtgtatt gccccggcga tgctgccgaa aattttcgat
    17461 ttcccgctgc gtttgaccat cggtactgat atcgataccg cagaagtgct ggaagagatg
    17521 ggcgcggagc atgtgccgtg tcctgtcgat gatatcgtgg ttgatgaaga caataagatt
    17581 gtcaccaccc cggcatatat gctggcgcag aacattgcag aagcggcgag cggcattgat
    17641 aagctggttt cccgcgtgct ggttctggct gaatgagtaa aagccgctta acggtgttta
    17701 gtttcgttcg ccgttttctt ctgcggttaa tggttgtcct cgccgttttc tggggcgggg
    17761 gcatcgcgtt gtttagcgtt gcacctgttc ccttctctgc ggtaatggtc gagcgacagg
    17821 tcagcgcctg gctgcatggt aattttcgtt acgtggcaca ttctgacagg gtcagtatgg
    17881 atcaaatctc gccgtggatg ggactggcgg tgattgccgc agaagatcag acatttcctg
    17941 agcactgggg ctttgatgtc gcttccattg agaaagccct ggcgcacaat gagcgcaatg
    18001 aaaaccgtat tcgcggtgct tcaacgattt ctcaacagac agccaaaaat ctctttttat
    18061 gggatgggcg tagctgggtt cgaaaagggc tggaagccgg attaacgctg gggatagaaa
    18121 ctgtctggag caaaaagcgt atcctgacgg tttacctgaa tatcgccgaa tttggcgacg
    18181 gtgtgtttgg cgtcgaagct gcggcacaac gttatttcca caaacccgcg agcaaactta
    18241 cccgatcgga agctgcctta ctggcagctg tattacctaa tccacttcgt ttcaaagtct
    18301 ccgcgccatc gggctacgtg cgcagccgtc aggcgtggat tttacggcag atgtatcagt
    18361 taggcggtga gccgtttatg caacaacacc agctggatta agcttcgcgc gaagcgaggg
    18421 ccagcgtttt gctctcacga ggttgcagat ctttctgcag ggcaatagcc agcgccggtt
    18481 gggcgataaa tcgtttccac aaccgctctt gcttacgcag acaacaccac tttgaccagg
    18541 tcgccagtgg aatcaatgtg ctttcaccaa tgttgtcgca gacgacgggg ataacctctg
    18601 actcactaat gcggtgacag aggatattct gcggcttcag cgacatcgtc acgatacggt
    18661 tatcctgcaa ataacgcttc agctgtttca gtaattggcg cagttgtgca acgtcttctt
    18721 cgtaacggca ttgttcagca aattcggtca gcgtaatgga gggcttgccg tcaaaatcag
    18781 caatcacatc ataaacataa ccggttccgc aatccgtctc gacggtaccg tgatagcgcg
    18841 gtattccact ccagtctttt aaccggcgac caagatgcgc gtagtacttt aactcacgac
    18901 ggatctcttt atcgccacca tcgccacgat ggtagacaat cttgatacag cgttgggcat
    18961 cttccggatg cgcataacat ttccgatgtc ttccggtgcc caggggactt tgttcagata
    19021 agcgaatcat gccatctcct ggaaatattt tctgacgatc tccagtatgg tcgattaact
    19081 taaacgaaac ctaaacaatg cttcttaagg ttttcttaag gttcttctga aagtaaaaag
    19141 cgcacgaaat gtacaattga cgttgattga gaacgatttc agtacgtgat tttcttattg
    19201 tcggggggag tttgaaggga gttgtatgtc aaagtgatga agattaatct tcatcaaaac
    19261 cagaattaaa gagggcgata acggcggcca gtgcttcctc ttcctgtgga ccggtcgctt
    19321 caacttcaat ctgccgtcct ttggcagaat ccaacatcag cagcgcaata acgctgttgg
    19381 cttcagcctc ggtgccttcg tcattacgta acagcacttc agcgtcaaaa ccctgcatta
    19441 attcaaacag cttcattgca ggccgggcat gcatgcccag cttgtttgtg atttcaacag
    19501 tttgcttgac agtcatggtt tacgtttttc cagcgtacga tggcgtgact ggacgttttt
    19561 accgcgcgag cggaagtagt ctgccagttg ctctgcaata tacaccgaac ggtgcttccc
    19621 gccggtacaa ccaatggcga ccgtcaagta gctacggttg ttggtttcca gcataggtaa
    19681 ccatagctca agatagcttc gcgtctggta gataaaattg tgtacttctg tgtggcggtc
    19741 gaggaacgcg gcgacaggct tatcaagacc tgtcattgga cgcagtttcg gatcccagtg
    19801 cgggttcggc aagaagcgca cgtcaaagac gtaatctgca tcgataggga taccgtgttt
    19861 gaagccgaaa gactcaaaga ccattgtcag ttcgcgttca cgtttaccca gcagacgggt
    19921 acgcagcatt tctgccagct cgtgaacgga catttctgag gtatcgacaa tcagatccgc
    19981 tcgcgaacgc agaggctcca gcagatcgct ttctttgtcg atagcacttt ccagcgacag
    20041 gtttttgctg gaaagcggat gcagtcggcg cgtgtcactg taacgacgaa ttaaggtatt
    20101 acggtcggca tccaggaaca gtagttgcgg tgagaaagcg tcaggcaggt tactcatcgc
    20161 ctgttcgaat atttctggtg actccggcat attacgaaca tcaatgctga cggcggcaga
    20221 aatctcgcga tcggccaggg ttcgagccag atcgggtaac aacactacgg gaaggttatc
    20281 cacgcagtaa aaacccatat cttccagcgc acgcagggcg acagatttac ctgaacctga
    20341 acgtccgctg acgatcatca gtaccatgta ctgtttctcc tcacaacgtc taaaagagac
    20401 attaccgaat aactacgctt catccggagt acctttggta tccgtaatga tttgatacag
    20461 ctcttcatcg ctctgggctg cgcgtaaacg gcggcagatg gttttgtccg ccagacgttt
    20521 cgccaccagc gacagagtat gcaggtgcgt tttagtttgg tctgccggca ccagcagggc
    20581 aaaaagtaag tccaccggtt ggttgtcgat ggcatcgaaa gctataggcg tttcgagctg
    20641 aacgaaaacg ccaacggcac gcagagtatc ttcttccagt ttgccatgcg gaatggcaat
    20701 accattgccg ataccggtac tgcccatttt ttcccgcgtc aggatagctt caaaaaccac
    20761 ctgaggcggt aggctaagtt gtttcgccgc cagctcgctg atgatttcca gggcgcgttt
    20821 tttgctctga cagtggacgc ggcttcgcgt acattccctg ttaagaacac tgctaagctg
    20881 tagagttgta tcattatttg tcataatttc acctaagaac ctgcccactc aaacagtgga
    20941 acaggctcgg agcattaacc gtacaaatgg cccgttgtgc cgcacaacag gccgccatgc
    21001 acatgctaat tgcccggaca attagtgttg tttcagttta tctttatgtt tggtcaactg
    21061 gcgtgccagc ttatcaatta agccatcaat ggcagcgtac atgtcctgac cttccgcgct
    21121 ggcatgaatt tcaccaccgt ttacatgcag tgttgcatct gaggtgtggg tgactttctc
    21181 cactttcaga acaacataga cctggttgat tcggtcaaaa tattgctcaa gtttggcaaa
    21241 tttggctgta acaaattcgc gcagtgcctc ggtgatctcg acgttatttc cggtaatgtt
    21301 gagctgcata gtgtcttcct tatcggttgg gtcaaacgag ctgtttacgc tggtttgacg
    21361 gcggaatgga taaagactct cggtacttcg caacagtgcg gcgtgccacc atgatacctt
    21421 gttccgacag caaagaggtt aacttgctgt cgctcaacgg tttcgctggg ttttccgccg
    21481 cgattaattt cttcaccagc gcacgaatcg ccgtggagga agcttcgccg ccgccctcgg
    21541 tattgacgtg actggaaaag aaatacttca gttcaaaaat gcctcgtgga ctatgcaggt
    21601 atttttgcgt ggtcacgcga gatatcgtcg attcatgcat ttcgacagcc tgggcgatat
    21661 cggccagtac catcggtttc atatattctt cgccttgctc aaagaaggct tgctgctgtt
    21721 caacgataca gcgactcacg cgcagtagcg tatcgttacg gctttccaga ctcttgatca
    21781 accatttggc atcttgcaga ttgctgcgga taaactggct gtcaccatca ttgcgcgcgt
    21841 tattgcacat cgaggcgtag tgctggttga tttgcagacg aggaatgctg tcactgttga
    21901 gttctaccgt ccagtgaccg ttatgcttac gcaccagcac atctggaatg acatactcag
    21961 gttcgccagt ctggatcgat tgcccggggc gcggatcgag cgactggatc agattgacgg
    22021 cttctttcag cacatcttct ttcagacgcg tgacgcgcat taaagtgcgg aagtcgtgat
    22081 tggctaacag atcgagatga tcgctaatga tcagtctggc ctcttccagc cacggcgtgg
    22141 ttttatcgaa ttgggagagt tggatcagca ggcagtcacg cagatctttt gccgccacac
    22201 cgaccggatc aaaccgttgg atccgcttaa ggacggcttc aacttcgtcg atgtcgatct
    22261 cttcatcgcc catactttcg agaatatctt ccagcgggac agtcagataa ccggtgtcat
    22321 caacggcatc gacgatagag gtagcaatag cgcggtcagt gtcggaaaac ggtgtcagct
    22381 cgacctgcca catcaggtaa tcctgcaagg tctgcgtcgt ttcgccctga tagaccggca
    22441 gctcgtcgtc aatgtagtca ccgctggtgc cggatggtgt accagcggtg taaatggtgt
    22501 cccaactggc atcgagcggc agctcttccg gcatctcttt ttgttcgagc gcgtcggcgg
    22561 tgtccagcgt ttcactgtct tgcgtttcgc gggtgtcgat ttcttcatga gtgtcgattt
    22621 gctcaagcag cggattactc tccagcgcct gctgtagctc ctgctgaagt tccagcgtcg
    22681 acaactgcaa cagacgaatt gcctgttgga gctgtggcgt catcgccagt tgttggctaa
    22741 gcctgagttg caaaccttgc ttcatgttca gaatcgtact ctcctgctaa aacgtcgcaa
    22801 acttctaccc tatcagagtc tgaagtcttc cccaaggtat acacgcttaa cgtgttcgtc
    22861 ttgtaagatt tctgtaggcg tgccgtgggc gatcaaatgc ccctgactga cgatataagc
    22921 gcgttcacaa accgccagtg tttcacgcac gttgtggtca gtgatcagca cgcccaagcc
    22981 gctgtcgcgc aggtgctcaa tgatgcgttt aatgtcgata actgagatcg ggtcaacccc
    23041 ggcaaacggt tcgtcgagca gaataaattt cggattcgca gccagtgcgc gggcaatttc
    23101 tacacggcga cgttcacccc cggagagtga ctgccccatg ctgtcacgca ggtgctcaat
    23161 gtgaaactct tccatcagct cgttcgcgcg gtcttcacgc tgttcagcag acaagtcgtc
    23221 acgaatttgc agtaccgcca tcaggttatc gtaaacgctg aggcgacgga aaatggaggc
    23281 ttcctgtggc agatagccga taccgcggcg cgcgcgtgca tgcagaggta gcagactgat
    23341 atcgtcatca tcaataatga tgttgcccgc atcgcgcggc acaatgccta caaccatgta
    23401 gaaagtggtg gtcttaccgg caccgtttgg ccccagcaga ccgacaattt ccccggagtt
    23461 gacggtcagg ctgacgtctt ctaccacgcg acggccttta taggcttttg caaggttctt
    23521 tgcagttaat gttgccataa cgaattaatt acccttcttc tgtgccgggg tctggccttt
    23581 gttgtttttg tcctgcagct gcgacggcac cagaacggtg gttacacgct tgcctttgtc
    23641 gctgaaagcc tgcattttct gctctttcac caggtaagtg atcttatcgc ccttaatgtt
    23701 gctatcgacc tgctgcagat aagcattacc cgtcagaacg acaaaatctt ttgccagttc
    23761 gtagtgcatc tgggaagcgt gaccttcaac gggtttaccg ttgtcctgca tctggtagaa
    23821 cgttgccggt ttaccgtagc cgtcaatcac ttctttacct tgttcgccac ccggacgggt
    23881 aacgaccact ttgtcggcat taatttttat ggtgccctgg gtgacgatga cattaccggt
    23941 aaaggtaacc acgttgcctt gcatatcaag agattgctgg tccgattcaa tgtgtatcgg
    24001 ctgatcagtg tctccggtta cggcaaatgc cggaatgctg gcggccagaa gtgagctggc
    24061 aagcacaaga ttaaggctga gtttgtttgt tttgaatttc ataggatgtt ctaacctttt
    24121 caatcagctc ggcgttcttg ctgcgtaagt tgccgcgcat tttcagaccg ctggagttaa
    24181 atgttgttcc gtataacgtg acgaggtctt cagaggtaac atcctgcgtc accagattga
    24241 tctgcgcgtt atccgtcgtg attctgcgaa gttgagagtc cggcacaagt gcgttgactt
    24301 caacgtgtcc atataaatag agcatccggt cattggtcag cttggcttta tctgctttta
    24361 cggaccatgt cgggatttta tccttatcaa acgtggtaag taccggctgc gtaaaccacg
    24421 aaacggcctg atcggaataa tattcaacgt gttgagcaat caatcgatag cttagcgccc
    24481 cttctgggtt atagacgagc gtgtccgtat gctcgctttt ataggtggga tcattgttgt
    24541 tgacgaccac ctgggcggta tcgtcttttt cggccatatt aataccgatc atcaccagaa
    24601 ccgccagtga tagcacaatg ataacccaac gtctggcttt actcatatcg attgcccttt
    24661 ggcttcatcc agtttgccct gcgccaggag taataagtcg caaacttctc gcactgcgcc
    24721 acgaccgcca gcaatgcgcg tcacgtaatc ggcgcgcggg atcaacagtg gatgcgcatc
    24781 ggccacggcg acgcttaaac ccactttttc cattaccggc caatcgatga gatcatcgcc
    24841 gacataagcc acattttccg gggcaatcgc caatttttcc agcagatcgc taaaggcgat
    24901 cagtttgttt gactgcccct gatacaagtg agtgatcccc aatgtggcac aacgatcttc
    24961 taccagttta gcctttcgcc cggtaatgat agcgacttca atatcagagg tgagcgcaca
    25021 acgaatgcca taaccgtcac gaacattgaa cgctttcagc tcttcgccgt tattgcccat
    25081 ataaatcagg ccatctgaca gtacgccatc aacgtcgagg attagcagac gaatgttctc
    25141 tgcttttgct ataacgtcgg cgctgacagg gccgtaacag gtcgcaagcg acgcacctgc
    25201 tttgctcatt gttgtttatc cttgaatctt tacactacgc ctgcacgcag taaatcatgc
    25261 atatgtaaca caccgagtaa atggtcgcca tcggcaacca tcacggaggt gatatggcgg
    25321 gactgcatta agttcagtgc ctcaacggcc agaatgccag ggcgcacgcg tattcccccc
    25381 ggcgtcatca catcggcaat acttaactga cgaacatcca cgcccatatc gaagacacgg
    25441 cgtaaatcac cgtcggtaaa gatgccttca atcatcatat tgtcatcgca aatgacagtc
    25501 ataccaagat ttttgcgggt aacttccagc aatgcgtcac gcagactggc cgttttctta
    25561 acatgcggga tctcatcgcc cgtatgcata atatcgttta cgcgcagcag aagtttacga
    25621 cccagtgcgc cgcctgggtg tgagagcgca aaatcttcag cagtaaagcc gcgtgctttt
    25681 aacagcgcga cagcgagggc atcgcccata accagcgtgg cggtggtgct gctggtcggt
    25741 gccagcccta acggacaggc ttctttcgct actttaacac acagatgcac atctgcggcg
    25801 cgcgccatgc tgctctccgg gcgaccggtg atgcagatta acggtatgtg aagacgctta
    25861 agcactggaa ttaaggcagt gatttcgctg gattcacctg agttagagat agcaatcacc
    25921 acatcctgtg gggtgaccat gcctaaatca ccatgcgcgg cttcaccagg atggacgaaa
    25981 aatgaaggtg taccggtgct ggcaaacgtt gcggccattt tgcgcccgat gtgccccgat
    26041 ttccccatcc ccatgacgac aactttccct ttacaccaga acatcttttc acaggcaagc
    26101 gtgaaattct gattgatgta ttgatcaagc tccgccaggc attcacgttc aatcgccagg
    26161 acttctttac ctgcttgctg aaagtcaaaa cccggttgta actctacgtg cgacataatg
    26221 cgtttccagt tattcaacga gtattggcga taaccagtac aacatcgcca gccatacgat
    26281 aaatccgcca gttaataata cccctacacc acggcccggt tgcggggagc gccgccagca
    26341 cagcaacgca aaaataatgc tcaccagcaa catcacgctg tagtcacgac tgtacgccag
    26401 tggatcaatc tctcctggcg ttatcagcgc gggtaaaccc aacacgatga caatattaaa
    26461 aatgtttgcg ccaatgatat ttccgacagc aatgtcgttt tcacctttgc gaacgcccgc
    26521 tattgcggtt gccagttccg gcaggctggt tccgatagca attgccgtca gacccatcgt
    26581 caactcgctg atggcaaagt aattcgccag caccgtggcg ttatcaacca ccatccgcgt
    26641 ggccactggc atgatgataa gcgcaatgcc gagccataaa aacgcgacgg gcaatccgcc
    26701 gtcacgcggc agctctgcaa gctgctctct ggtcaggctg tcagtcccct gacgttcagc
    26761 ctgacgtgca agtttaacaa tgaacagcag ccatagcaca gccagaaaga ggagaaagat
    26821 accatcgctg cgactaagtt gtccgtcata gagtacggaa ccggccacca cgctgaccaa
    26881 caacattaag ggtaattcac ggcgtagaac atcggaatgg acggtaaaag gacgaaccag
    26941 cgcagccaga ccgaggatca gcaatatatt gataatgttt gagccgaggg ctgtaccaac
    27001 ggctaaatcg cgttgttcgt gcagagacgc ggcaagcgag acgatgattt ctggtaacga
    27061 tgtaccaata ctgaccaccg tcatgccgat gatcagcggc gggatgccaa aggttcggca
    27121 aagaatagac gcggcaaaaa ccaggcggtc ggcactgtaa acgaccaaaa gtaaaccaac
    27181 aattaacagt gccgtagcta aaagcatcta acgtcctttc ttcaggtata ctcgccggtc
    27241 cgctgaagat tttcagaaag ccgtaacgga ttcttaattt tgactttatg cggctaaaaa
    27301 gtaaaacaaa tgccagcttt cgctaaccac ggcgggtaat attctgtaaa tatgttgggt
    27361 tcaaggttaa attgagcgcc atgcttagaa aatcaacgca agacgaaggg tgaattatgg
    27421 agcagtctgt ggcgaattta gtcgatatgc gcgatgtcag ttttacgcgt ggcaatcgct
    27481 gcatcttcga taatatttcc ctgaccgtgc cgcgagggaa gatcacggcg atcatggggc
    27541 catcgggcat cggtaaaacg acgctactcc gtctgattgg cgggcaaatc gcaccagatc
    27601 atggtgagat ccttttcgat ggtgagaata ttccggcgat gtctcgttcg cgcctgtata
    27661 cagtgcgcaa acggatgagc atgttatttc agtccggggc gttgttcact gatatgaacg
    27721 tatttgacaa cgtcgcctat ccactgcgcg aacataccca acttcccgcg ccattgttgc
    27781 atagtacggt gatgatgaag ctggaggccg tggggctgcg tggagcggct aaactaatgc
    27841 cttctgaact ttccggtggg atggcgcggc gtgcagcgct ggcgcgtgcg attgcgctgg
    27901 agccggatct catcatgttt gatgaacctt ttgttgggca agatcccatc accatgggcg
    27961 tactggtgaa gctgatttct gagctgaaca gcgcgctggg cgtgacttgt gtggtggttt
    28021 ctcacgatgt gccggaagtg ttaagtattg cggatcacgc ctggatcctg gcggacaaaa
    28081 aaattgtcgc tcatggcagt gcccaggcgt tgcaggcgaa tcctgatccg cgcgtacgtc
    28141 agtttctgga cgggatagct gacgggccgg ttccgttccg ctatcctgcc ggcgattatc
    28201 acgctgatct tttaccaggg agttaagcca ctcatgctgt taaatgcgct ggcgtcgctc
    28261 ggacataaag ggattaaaac cctgagaacg tttgggcggg ccgggttaat gttattcaat
    28321 gcgctggtcg gcaaaccgga atttcgcaaa catgcgccgc tgctggtgcg ccagctctat
    28381 aatgtcggcg tcctgtcgat gctgattatt gtagtttctg gcgtgttcat cggaatggtg
    28441 ttggggctgc aaggttatct ggttctgacc acttatagtg cggaaaccag tctgggtatg
    28501 ctggtggcgt tatcgctact gcgtgaactg gggccggtgg ttgccgcgtt gttgtttgcc
    28561 gggcgtgctg gttcggcgct aaccgcagaa atcggcctga tgcgcgctac agagcaactc
    28621 tccagtatgg agatgatggc ggtggatccg ctgcgtcggg ttatttctcc ccgtttctgg
    28681 gctggggtta tttcattacc actgttgacg gttatcttcg tcgccgtggg gatctggggc
    28741 ggatcgctgg tcggcgtcag ttggaaaggc attgatagcg ggttcttctg gtcggcaatg
    28801 caaaatgccg tcgactggcg tatggatctg gtcaactgtc tgattaagag cgtggtgttc
    28861 gccatcacgg tgacgtggat ttcgttgttt aacggctacg acgccatccc gacgtctgcc
    28921 gggattagcc gggcaaccac tcgcaccgtt gtccactcgt ctctggctgt tctggggctg
    28981 gattttgtgc tgaccgcatt gatgtttggg aattgagttc atgcaaacga aaaaaaatga
    29041 aatttgggtg ggtatctttt tattagcagc actgctggcg gcgctgtttg tttgcctgaa
    29101 ggcggcgaac gtgacgtcaa tacgtactga accgacctac acgctttatg cgacgttcga
    29161 taacattggc ggcctgaaag cgcgctctcc ggtcagtatt ggtggcgttg ttgtgggccg
    29221 ggtggcggat attacgctgg acccgaaaac ctatctgccg cgcgtaacgc tggaaattga
    29281 acaacgttat aaccatattc ctgataccag ttcgctgagc attcgtactt ccggcctgct
    29341 gggggaacaa tatctggcat taaacgtcgg ttttgaagac ccggaactgg ggactgctat
    29401 cctgaaggat ggcgatacaa ttcaggacac caagtctgcg atggtgctgg aagatctcat
    29461 tggtcagttc ctttacggta gtaaaggcga tgacaataag aatagtggcg atgcgccagc
    29521 tgctgcgcca ggtaataatg aaaccactga acctgtgggt acaacgaaat aatttcagga
    29581 gaaccgacgc atgtttaaac gtttaatgat ggtcgctttg ctggtgattg caccgctgag
    29641 tgcggcaacc gcggcagacc agaccaatcc gtataagctg atggacgagg cggcgcagaa
    29701 aacgttcgat cgcctgaaga atgagcaacc gcaaattcgg gccaacccgg attatctgcg
    29761 taccattgtt gatcaggaac tgctgccata cgtacaggtg aaatacgccg gtgcgctggt
    29821 gctgggccag tattacaaga gtgcgacccc agctcaacgt gatgcttact ttgccgcttt
    29881 ccgtgagtac ctgaagcagg cttacggtca ggcgctggcg atgtatcacg gtcaaaccta
    29941 tcagattgcg ccagaacagc cgctgggtga taaaaccatt gtgcctattc gcgttaccat
    30001 tattgacccg aatggccgcc cgccggtgcg tctggacttc cagtggcgta aaaactccca
    30061 gacgggcaac tggcaggctt acgacatgat tgctgaaggc gtcagtatga tcaccaccaa
    30121 acaaaacgag tggggaacgc tgctacgtac caaaggtatc gacggcctga ctgcgcaact
    30181 gaaatcgatt tctcaacaga aaatcactct ggaagagaaa aaataatgag cgagtcactg
    30241 agctggatgc agacgggtga cacactggcg ttatccggag agctggatca ggacgttctg
    30301 ctaccgcttt gggaaatgcg tgaggaagcg gtgaagggga ttacctgcat cgatcttagc
    30361 cgtgtgtccc gcgtggatac gggtggactg gcactgctgc tccatcttat cgatctggcg
    30421 aaaaagcagg gcaacaacgt gacgcttcag ggggtaaacg acaaagtgta taccctggcg
    30481 aaattgtata atttgcctgc tgatgttctg cctcgttaaa ttttttcagg ccggatatgg
    30541 cgttaaccgc ctgtccggca aacctgcctg caagtcgagc cttgtctgat ttatcagcga
    30601 ggctttttgc ttatttatgc cagcgcaact ttgctctaag atgtttcgct ggtttattaa
    30661 ctgatgattg aagatcccat ggaaaataat gaaattcaga gcgtgttgat gaacgctctc
    30721 tccctccagg aagtccacgt ttccggcgat ggcagccact ttcaggttat tgccgtgggt
    30781 gagttgtttg acggcatgag tcgggttaaa aaacagcaga cggtctatgg tccgctgatg
    30841 gaatatattg cggataaccg cattcatgct gtgtcgatca aagcgtatac ccctgcggag
    30901 tgggcgcgcg atcgcaaact gaacggcttt tgagctatgg gcgattcgcc ggtagcggat
    30961 atgaattgtt aactgagaac aaactaaatg gataaatttc gtgttcaggg gccaacgaag
    31021 ctccagggcg aagtcacaat ttccggcgct aaaaatgctg ctctgcctat cctttttgcc
    31081 gctctactgg cggaagaacc ggtagagatc cagaacgtcc cgaaactgaa agacgtcgat
    31141 acatcaatga agctgctaag ccagctgggt gcgaaagtag aacgtaatgg ttctgtgcat
    31201 attgatgccc gcgacgttaa tgtattctgc gcaccttacg atctggttaa aaccatgcgt
    31261 gcttctatct gggcactggg gccgctggta gcgcgctttg gtcaggggca agtttcacta
    31321 cctggcggtt gtacgatcgg tgcgcgtccg gttgatctac acatttctgg cctcgaacaa
    31381 ttaggcgcga ccatcaaact ggaagaaggt tacgttaaag cttccgtcga tggtcgtctg
    31441 aaaggcgcac atatcgtgat ggataaagtc agcgttggcg caacggtgac catcatgtgt
    31501 gctgcaaccc tggcggaagg caccacgatt attgaaaacg cagcgcgtga accggaaatc
    31561 gtcgataccg cgaacttcct gattacgctg ggtgcgaaaa ttagcggtca gggcaccgat
    31621 cgtatcgtca tcgaaggtgt ggaacgttta ggcggcggtg tctatcgcgt gctaccggat
    31681 cgtatcgaaa ccggtacttt cctggtggcg gcggcgattt ctcgcggcaa aattatctgc
    31741 cgtaacacgc agccagatac tctcgacgcc gtgctggcga aactgcgtga cgctggagcg
    31801 gacatcgagg tcggcgaaga ctggattagc ctggatatgc atggcaaacg tccgaaggct
    31861 gttaacgtac gtaccgcgcc gcatccggca ttcccgaccg atatgcaggc ccagttcacg
    31921 ctgttgaacc tggtggcaga agggactgga ttcatcaccg aaacggtctt tgaaaaccgc
    31981 tttatgcatg tgccagagct gagccgtatg ggcgcgcacg ccgaaatcga aagcaatacc
    32041 gttatttgtc acggtgttga aaaactttct ggcgcacagg ttatggcaac cgatctgcgt
    32101 gcatcagcaa gcctggtgct ggctggctgt attgcggaag ggacgacggt ggttgatcgt
    32161 atttatcaca tcgatcgtgg atacgaacgc attgaagaca aactgcgcgc tttaggtgca
    32221 aatattgagc gtgtgaaagg cgaataatcg tctgagagct ggcagccccg ttcgcggggc
    32281 taccgccaga ccactcattt tttcggttta gtgtagctac gcatcaactg cgttctgtcg
    32341 ataaactcat gggtctgcgg atcatggtag cgtgatggcc agataaccca ggggtcagtt
    32401 cccagggctt tcgcaataat catctctcct ttcggccatg ggcgcgataa tgcattcgcc
    32461 agcgttgagg aactcaaacc atttctgcga gactccgccg ccattgatgt tcccttcttg
    32521 cgcaaacccg caatgatgtc agcgggatgc cagtcaatga aattactttc catctttcct
    32581 cactccttaa gttagtcgct ttcattcaga acacttcttc tgtcgagcat cactatactc
    32641 ctgatggccg attgctctaa aaagttccta ta
//
LOCUS       DAZFKY010000034        32436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFKY010000034 DAZFKY010000000
VERSION     DAZFKY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..3251)
                     /gene="acrB"
                     /locus_tag="ACHKCL_001426"
     CDS             complement(102..3251)
                     /gene="acrB"
                     /locus_tag="ACHKCL_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5762427.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3274..4467)
                     /gene="acrA"
                     /locus_tag="ACHKCL_001427"
     CDS             complement(3274..4467)
                     /gene="acrA"
                     /locus_tag="ACHKCL_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5762428.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4609..5256
                     /gene="acrR"
                     /locus_tag="ACHKCL_001428"
     CDS             4609..5256
                     /gene="acrR"
                     /locus_tag="ACHKCL_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5762429.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPSDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5384..8746
                     /gene="mscK"
                     /locus_tag="ACHKCL_001429"
     CDS             5384..8746
                     /gene="mscK"
                     /locus_tag="ACHKCL_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5762430.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8785..8949)
                     /gene="rsmS"
                     /locus_tag="ACHKCL_001430"
     CDS             complement(8785..8949)
                     /gene="rsmS"
                     /locus_tag="ACHKCL_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5762431.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8963..9490)
                     /gene="priC"
                     /locus_tag="ACHKCL_001431"
     CDS             complement(8963..9490)
                     /gene="priC"
                     /locus_tag="ACHKCL_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5762432.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9560..9937
                     /gene="ybaN"
                     /locus_tag="ACHKCL_001432"
     CDS             9560..9937
                     /gene="ybaN"
                     /locus_tag="ACHKCL_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5762433.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10090..10641
                     /gene="apt"
                     /locus_tag="ACHKCL_001433"
     CDS             10090..10641
                     /gene="apt"
                     /locus_tag="ACHKCL_001433"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5762434.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10770..12701
                     /gene="dnaX"
                     /locus_tag="ACHKCL_001434"
     CDS             10770..12701
                     /gene="dnaX"
                     /locus_tag="ACHKCL_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5762435.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12034..12098
                     /gene="dnaX"
                     /locus_tag="ACHKCL_001434"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12754..13083
                     /gene="ybaB"
                     /locus_tag="ACHKCL_001435"
     CDS             12754..13083
                     /gene="ybaB"
                     /locus_tag="ACHKCL_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5762436.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13083..13688
                     /gene="recR"
                     /locus_tag="ACHKCL_001436"
     CDS             13083..13688
                     /gene="recR"
                     /locus_tag="ACHKCL_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5762437.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13798..15672
                     /gene="htpG"
                     /locus_tag="ACHKCL_001437"
     CDS             13798..15672
                     /gene="htpG"
                     /locus_tag="ACHKCL_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5762438.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15853..16497
                     /gene="adk"
                     /locus_tag="ACHKCL_001438"
     CDS             15853..16497
                     /gene="adk"
                     /locus_tag="ACHKCL_001438"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5762439.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16629..17591
                     /gene="hemH"
                     /locus_tag="ACHKCL_001439"
     CDS             16629..17591
                     /gene="hemH"
                     /locus_tag="ACHKCL_001439"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5762440.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17588..18547)
                     /gene="aes"
                     /locus_tag="ACHKCL_001440"
     CDS             complement(17588..18547)
                     /gene="aes"
                     /locus_tag="ACHKCL_001440"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5762441.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18699..20003
                     /gene="gsk"
                     /locus_tag="ACHKCL_001441"
     CDS             18699..20003
                     /gene="gsk"
                     /locus_tag="ACHKCL_001441"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5762442.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20136..21812)
                     /gene="ybaL"
                     /locus_tag="ACHKCL_001442"
     CDS             complement(20136..21812)
                     /gene="ybaL"
                     /locus_tag="ACHKCL_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5762443.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22049..23269)
                     /gene="fsr"
                     /locus_tag="ACHKCL_001443"
     CDS             complement(22049..23269)
                     /gene="fsr"
                     /locus_tag="ACHKCL_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5762444.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23487..25139
                     /gene="ushA"
                     /locus_tag="ACHKCL_001444"
     CDS             23487..25139
                     /gene="ushA"
                     /locus_tag="ACHKCL_001444"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5762445.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25176..25655)
                     /gene="ybaK"
                     /locus_tag="ACHKCL_001445"
     CDS             complement(25176..25655)
                     /gene="ybaK"
                     /locus_tag="ACHKCL_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5762446.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25859..26653)
                     /gene="ybaP"
                     /locus_tag="ACHKCL_001446"
     CDS             complement(25859..26653)
                     /gene="ybaP"
                     /locus_tag="ACHKCL_001446"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5762447.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26791..27132
                     /gene="ybaQ"
                     /locus_tag="ACHKCL_001447"
     CDS             26791..27132
                     /gene="ybaQ"
                     /locus_tag="ACHKCL_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5762448.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27347..29851)
                     /gene="copA"
                     /locus_tag="ACHKCL_001448"
     CDS             complement(27347..29851)
                     /gene="copA"
                     /locus_tag="ACHKCL_001448"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5762449.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30113..31045
                     /gene="glsA"
                     /locus_tag="ACHKCL_001449"
     CDS             30113..31045
                     /gene="glsA"
                     /locus_tag="ACHKCL_001449"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5762450.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31048..32340
                     /gene="ybaT"
                     /locus_tag="ACHKCL_001450"
     CDS             31048..32340
                     /gene="ybaT"
                     /locus_tag="ACHKCL_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5762451.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKRMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7856 a         8572 c         8527 g         7481 t
ORIGIN      
        1 tttcgtatga gatcctgagt tggtggttca attactcctt aatgttcgta ggttatgcat
       61 aaaaaaggcc gcttacgcgg ccttagtgat tacacgttgt atcaatgatg atcgacagta
      121 tggttgtgct cgatatcttc attcttgcgg ctaaagcggc ggcgaaccac cacaaagaat
      181 accggaacga agaagattgc cagtaccgtt gcggtcacca tcccgcccat tacaccggta
      241 cctactgcgt tctgcgcgcc ggaaccagca ccagtactga taaccagcgg cataacgccg
      301 aggataaacg ccagcgaggt catcaggatc ggacgtaaac gcatccgcac cgcatcaagc
      361 gtcgcttcaa tcagaccttt accttcttta tccatcaagt ctttggcgaa ttcgacgata
      421 agtatcgcgt tcttcgccga caacccaatg gttgtgagca ggcctacctg gaagtaaacg
      481 tcattggtca ggccacggaa ggtggcagcc agcaacgcac cgataacccc cagcggaacg
      541 accagcataa cggagaacgg aatcgaccag ctctcgtaca gcgccgccag acacaggaac
      601 acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt tgccggagag acgttcctga
      661 taggacatcc ccgtccagtc atagccaaca ccggtaggca gtttgctcgc cagttgttcc
      721 atcagctcca ttgcttcacc ggtactttta cccggtgccg cctggcctaa gatttccatg
      781 gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt actcccaacg agaagaggag
      841 aacgccgaga atggcaccat ctgaccatca gcagcacgaa cataccagtc gccgatatca
      901 tccggcagca tacggtattt cgcttctgac atgacgtaaa ctttcttcac acgaccgcgg
      961 tcgataaagt cgttcacata gctgccgccc catgcagcgc ccagagtggt gttaatgtcg
     1021 ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga tatcaatctt aaactgcggg
     1081 gtatcttcca gaccgtttgg acgtacgctg gtcaacatat cagggtgctt cgctgcttct
     1141 gcaagcaact ggttacgcgc ctgagtcagt ttttcgtgac caaggccagc ctggtcaatc
     1201 agctcaaagt caaagccggt ggcggtaccc agttccacga ttgcgggcag gttaaaggcg
     1261 aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg cacgcatggt aatcgcttca
     1321 actttgtttt cttcacccgg acgatcggcc cagtccttca aggaaacgaa cgcaataccg
     1381 gtattctgac cacgtcccgc aaagccgaag ccgttaacgg cgaacaccga ctcaacgttg
     1441 ttcttttctt tggtcagata gtaatgcgtt acctcattga gcactttctg cgtacgttcc
     1501 tgcgttgcac ctgctggcag ctgaaccatg gtcataaata cgccctggtc ctcatctggc
     1561 aagaaggagc ttggcagacg cacaaacaga taggccatgc cgaccacgat gatcagatac
     1621 agcaccagat aacgccccgt actgcgcaga ataccgccta cgctgtcggt gtagtggtgc
     1681 gtgctcttct cgaacatgcg gttaaaccag ccgaagaagc cttttttacc ttccccgtga
     1741 tcgcctttgg caatcggttt cagcatggtg gcacaaagag ctggagtcag gatcaacgcc
     1801 accagtaccg acagcgccat tgctgaaaca atggtaatag agaactgacg ataaattgcc
     1861 ccagtagaac cgccaaagaa ggccatcggt acgaataccg ccgacagtac catcgcgata
     1921 ccgaccagag cgccctgaat ctgccccatc gacttacggg tggcttcttt tggcggcaaa
     1981 ccttcttccg ccatcacacg ctcaacgttt tctaccacaa cgatagcgtc atccaccaac
     2041 aggccgatgg cgagcaccat cccgaacatt gttagcgtgt ttatcgagaa gccaaaggcg
     2101 gcaaggacgg caaaggtccc gagcaatacc accggtacgg caatggtcgg aatcaacgtc
     2161 gcgcggaagt tctgcaggaa cagatacata accaggaaca cgaggatgat cgcttcgacc
     2221 agcgttttaa ccacttcgtg aatagagatt ttcacgaacg gcgtggtgtc gtacgggtaa
     2281 acaattttca gacccgacgg gaagaacggt tccatcttcg ccagttcagc acggattgcc
     2341 gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga tccccagacc ggaagccggt
     2401 tggccgttaa actctgcgat gatgtcgtag ttctcaccac ccagctcaat cttcgccaca
     2461 tcacgcagca gtacgcggga accatcctga ttcactttca gcaggatttt gccgaactct
     2521 tcagtagagg tcagacgcgt ctgagcaata atagaggcgt taagctgttg gcctttcacc
     2581 ggcggtgtac caccgagctg gcccgccgca acctgggcgt tctgcgcttt gatggcggta
     2641 ataacatcaa ccggcgttag ctggaatttg ttcagttcat tcgggttcat ccagatacgc
     2701 atcgcgtact gtgaaccgaa caactgaacg tcacccacgc cagacgtacg gctgatggca
     2761 tctttcatat tcgccgccac gtagtcggag atatcctcct gcgtcatggt gccatcggtg
     2821 ttgataacgc cgacaaccat caggaagctg ctggatgatt tctcaatgct caccccttgc
     2881 tgctgaactt cttgcggcag caacggcatc gccagctgca gtttgttctg cacctgaacc
     2941 tgcgcgatat ccgcatcagt accagactca aaggtcaggg tgatctgtac ggtacccgtg
     3001 gagtcactgt tagaggacat gtacatcagg ttatcgatac cgttcatatt ctgttcgata
     3061 acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc cggggtagga ggcggagatc
     3121 gttactgccg gcggtgcaat cgtaggatat tgcgccaccg gcagtttgag gatcgccagc
     3181 ccccctgcca acatgatgat aatggcgatc acccacgcaa aaatcgggcg atcgataaag
     3241 aaattaggca tgtcttaacg gctcctgttt aagttaagac ttggactgtt caggctgagc
     3301 accgcttgcg gcttgctggt tattatcagc ggtaacttct tgtgctttta cctggacacc
     3361 aggacgcact ttctgcagcc cacttattac tacgcgatcg cctgctttca gaccttctgt
     3421 caccagccac ttatcgccaa tagcctggct tgcaacgatc ggacgggttt ccactttgtc
     3481 atccgcgcca accaccagta cggtggcatc gccacgcggc gtacgggtta cgccctgttg
     3541 cgggactaaa atagcgtttg gattaagccc ttcttccaga cgtgcgcgca cgaacatacc
     3601 cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt agggtgatag acccagtggt
     3661 ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc tgcgggaact taatgccgtc
     3721 actggtgatc agcgacactt tggctttgcc gttctcttgt ttcagcgtgc cattcgccag
     3781 ttcctgtttc aggcgcagga agtcgttgct ggactgggtc acatcaacgt agatcggatc
     3841 aagttgctgc acggttgcca gcgcagtcgc ctgaccgttc tgtaccaatg cgccttccgt
     3901 cacgttcgac ttaccaatgc gaccgctaat cggagaggtg actttggtgt aagccagatt
     3961 gatccgcgca gtttcaacgg cagctttcgc cgcagttacc gcagcattcg cctgttgcgc
     4021 atcagccaga gcctgatcgt actcttgctt actgatgtac tgagtaccga gcagtttctg
     4081 ataacgattc accgtcaatt gcgcgatatt ggctgcagcc tgggctttcg ccagatcacc
     4141 tttcgcactg tcgtatgtcg cctgataggt cgcaggatca atctgataga gagagacacc
     4201 tgcttcgatg tcgctacctt ctttgaaatt acgcttcagg ataatcccgc taacttgagg
     4261 acgaacttct gcgatccggt aggcactggt gcgacccgga agctcggttg tgatctgcag
     4321 aggttcagtt ttgactgtta ctacgccaac ggcgggcatc tgctggccac cttgttgggc
     4381 ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag agcatcagaa cgaccgccag
     4441 aggcgtaaac cctctgtttt tgttcatatg taaacctcga gtgtccgatt tcaaattggt
     4501 caatggtcaa aagttaataa acccattgct gcgtttatat tatcgtcgtg ctatggtaca
     4561 tacattcaca aatgtatgta aatctaacgc ctgtaaattc acgaacatat ggcacgaaaa
     4621 accaaacaag aagcgcaaga aacgcgccaa cacatcctcg atgtggctct acgtcttttc
     4681 tcacagcagg gggtatcatc cacctcgctg ggtgagattg caaaagcagc tggcgttacg
     4741 cgcggtgcaa tctactggca ttttaaagac aagtcggatt tgttcagtga gatctgggaa
     4801 ctatcagaat ccaatattgg tgaactagag cttgagtatc aggcaaaatt ccctagcgat
     4861 ccactctcag tattaagaga gatattaatt catgttcttg aatccacggt gacagaagaa
     4921 cggcgtcgat tattgatgga gattatattc cacaaatgcg aatttgtcgg agaaatggct
     4981 gttgtgcaac aggcacaacg taatctctgt ttggaaagtt atgaccgtat agaacaaacg
     5041 ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt taatgacgcg tcgggcagca
     5101 attattatgc gcggctatat ttccggcctg atggaaaact ggctctttgc cccgcaatct
     5161 tttgatctta aaaaagaagc ccgcgattac gtcgccatct tactggagat gtatctcctg
     5221 tgccccacgc ttcgtaatcc tgccactaac gaataaccct gaatctgact ccaggatttt
     5281 tcctggacat tttcgtcgtt gctattctgg ttcactgcgt cgtgatattc ttgcggtttg
     5341 actttttcag gtcgttcttc aggttcagaa accttcattc atcatgacta tgttccagta
     5401 ttacaaacga tcacggcatt ttgttttttc agcatttatt gcttttgttt ttgtcttgtt
     5461 atgccagaac acggcgtttg cgcgggcgtc atcgaatggt gatctgccga caaaagcgga
     5521 cctgcaggcg caacttgact cactaaataa acaaaaagat ctttctgctc aggacaaact
     5581 ggtgcagcag gatctgacag atacattagc caccctcgat aaaatcgatc gcgtaaaaga
     5641 agagaccgtt cagctacggc aaaaagtcgc tgaagcgccg gaaaaaatgc gccaggcgac
     5701 cgcggcgtta acagcactta gcgatgtcga taacgacgaa gaaacgcgca aaattctgag
     5761 cacgctttcg ttgcgccagc tggaaactcg cgttgcccag gcgctggacg atttgcaaaa
     5821 cgcacaaaac gatctggcgt cttataacag ccagctggtt tcgttacaga cgcagcccga
     5881 acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg caacaaattc gcagtcgtct
     5941 ggatgggact gatgtcggcg agacggcctt acgtcccagc cagaaagtgt taatgcaggc
     6001 tcagcaggcg ttgctgaatg cggagattga ccagcagcgt aaaagcctgg aagggaatac
     6061 cgtcttgcag gataccttgc aaaagcaacg tgattacgtg acggcgaaca gcgcccgtct
     6121 ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc aagcgcctga ctttaaccga
     6181 aaaaacggcg caggaagccg tctccccgga tgaagccgcg cgtattcagg ctaatccgct
     6241 ggtgaagcag gaactggaaa ttaaccagca gttaagtcag cgtctgatta ccgcgactga
     6301 aaacggtaat cagttgatgc agcaaaacat taaagtcaaa aactggctgg agcgggcgct
     6361 gcaatcggaa cgcaatatta aagagcagat tgccgtcctg aagggcagcc tgctgttgtc
     6421 tcgtatcctt taccagcaac aacaaacgct gccctcggcg gatgaactgg aaaacatgac
     6481 caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt aaccagcagc gtgatgcact
     6541 cttccagagc gatgcgttcg tcaacaaact ggaagaaggt cacaccaacg aagtcaacag
     6601 cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt cgcgaattgc tggatcaact
     6661 caacaaacag ttgggtaacc agctgatgat ggccattaac ctgcaaatca accagcagca
     6721 gttaatgagt gtgtcgaaaa acctgaaatc catcctgact cagcaaatct tttgggtgaa
     6781 cagtaaccgt ccaatggact gggactggat caaagcgttc ccgcaaagcc tgaaagatga
     6841 atttaagtcg atgaaaatta cggttaactg ggaaaaagcc tggcccgccg tttttatcgc
     6901 tttcctcgct ggtttgccgc tgctgttgat tgccgggctg atccactggc gtctggggtg
     6961 gctgaaagcg tatcaacaaa aactggcttc cgctgtgggt tccctgcgta acgacagcca
     7021 gctcaacaca ccaaaagcga tccttatcga cctgatccgt gcactgccgg tgtgcctgat
     7081 tattctcgcg gttggcctga ttctgttgac catgcagctc aacatcagcg aactgctatg
     7141 gtcgttcagc aaaaaactgg cgattttctg gctggtgttt ggcctgtgct ggaaagtgct
     7201 ggagaagaac ggcgttgccg tgcgtcactt cggcatgccg gaacagcaga ccagccactg
     7261 gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct atccatttct ggtctgtggt
     7321 ggcagaactt tccccgctgc atctgatgga tgatgtgctg gggcaggcga tgattttctt
     7381 caacctgctg ctgattgcct tcctggtatg gccaatgtgc cgcgaaagct ggcgtgataa
     7441 agagtcgcac accatgcgac tggtcaccat taccgtgctg tcgattatcc cgattgcatt
     7501 gatggtgctg actgccacgg gttatttcta caccacgctg cgcctctccg ggcgttggat
     7561 tgaaaccgtt tatctggtga tcatctggaa cttgctctac cagacggtat tgcgcggctt
     7621 aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg gcgcgtcggc agaatctggt
     7681 gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc accattgcac tggaacaggt
     7741 taaccagcag acgctgcgta ttaccatgtt gctgatgttt gcgctgttcg gtgtcatgtt
     7801 ctgggcaatt tggtccgatt tgatcaccgt gttcagctat ctcgacagca ttacgctgtg
     7861 gcattacaac ggtactgaag ctggtgctgc ggtggtgaaa aacgtcacca tgggtagtct
     7921 gttgtttgcg attatcgcct caatggtggc ctgggcgttg attcgcaacc tgcctggttt
     7981 gctggaagtg ctggtgctct cgcgactgaa tatgcgccag ggcgcgtcgt atgccattac
     8041 taccatcctt aactacatca ttattgctgt tggtgcgatg acggtgttcg gatcgctggg
     8101 cgtctcttgg gataaactcc agtggctggc cgcagcatta tccgtaggtc ttggttttgg
     8161 tttacaagaa attttcggta acttcgtctc cggtttgatc attctgttcg aacgtccggt
     8221 gcgtattggc gatacggtaa ccattggtag cttctcgggg acggtaagta aaatccgtat
     8281 tcgtgcgaca acgattaccg atttcgatcg caaagaagtg atcatcccga acaaagcgtt
     8341 tgttaccgag cgtctgatca actggtcgtt gactgacact actacgcgtc tggtgatccg
     8401 tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt aaagtgttac tgaaggcggc
     8461 gactgagcac ccaagggtga tgcacgaacc aatgccggaa gtcttcttta cggcatttgg
     8521 tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt gaactgcgtg accgtagtcg
     8581 tactgtcgat gagctgaacc gtactatcga tcagctgtgc cgtgaaaacg acatcaacat
     8641 tgcctttaac cagcttgaag tgcatctgca caacgagaag ggcgatgagg tgacggaagt
     8701 aaaacgcgac tacaaaggcg acgatccgac gccagcggta gggtaaggac aataaagggc
     8761 gacagtgatg ccgcccttta ttttttactc gctttgtgaa gcctcatcgc tctgtaactt
     8821 cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtgcggg ctgggatctg
     8881 attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag gggcattttc
     8941 cagtgacata ctctctccat tgctagcggg ttaaacgcgc taacctgttt tcgatttttt
     9001 ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca cgatgcagcg
     9061 tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctacgttcg gcaaccatct
     9121 cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag cgggcaattt
     9181 ggggtggtgc gctatcccac tcgcgtagcg accaggctgt ggcttcacgc gctatagcct
     9241 ccagttgcgc cgccagatgt tctgccagcc aggcgacttg cggcagttgc tgttgctcaa
     9301 ctgcatgacg aagcgcagcc agattatcgc ccgccttgtc gagacaagcc tgtagtgttg
     9361 tcgcacgagt ctgaaaaaga tgcctgttga aacgagcact tagcgtggcg aactgtgaca
     9421 ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc agcagcaggg
     9481 cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt agccactctg
     9541 taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct ggctggcggt
     9601 agtgctgggc acgctgggcg tggtattacc ggtattaccg acgacgccgt ttatcctgct
     9661 ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc tgtaccgctc
     9721 atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc gcggcgtcaa
     9781 accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt tcgtccagat
     9841 gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct atatgtggcg
     9901 aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg cagttgcaat
     9961 tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg gtcaaaggtt
    10021 aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt aacaagcagg
    10081 catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag catcaaaagc
    10141 attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt actggaagac
    10201 ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa tgcgggcatt
    10261 aacaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt agctctgggt
    10321 ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga aaccatcagt
    10381 gaaacttacg atctggaata cggcaccgat cagctggaga tccacgttga tgccatcaaa
    10441 ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac tatcgaagcg
    10501 accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt cattatcaac
    10561 ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag ctacagcctt
    10621 gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta cggacagcac
    10681 aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc ccttcgagaa
    10741 tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc cgaaaatggc
    10801 gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg
    10861 gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt ggcgtcggaa
    10921 aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc attaccgcga
    10981 cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga
    11041 ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg ctggataacg
    11101 tccagtacgc tccggcgcgt ggtcgtttca aagtttatct catcgacgaa gtgcatatgc
    11161 tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg gagcacgtta
    11221 agtttctgct ggcgacgact gatccacaga aattgccggt gacgattttg tcgcgctgtt
    11281 tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg tcatcaactt gagcacatcc
    11341 tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgctggcg cgcgccgctg
    11401 aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc ggtgacggcc
    11461 aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat caggcgctgt
    11521 cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg attaatgaag
    11581 ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc ctgttgcatc
    11641 gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc gccatcgagc
    11701 tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt tactatcaga
    11761 cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg ggcgttgaga
    11821 tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag ccagaagtgc
    11881 cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc caggtgccgc
    11941 cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc
    12001 aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga
    12061 gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg
    12121 cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgccag
    12181 ctaaaaaaga agcgtatcgc tggaaggcga ccactccagt gatgcagcaa aaagaagtgg
    12241 tcgccacacc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg
    12301 cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggctcaggtg agtcaacttt
    12361 cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg
    12421 cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa
    12481 aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact atagttgaag
    12541 atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac
    12601 ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggttct
    12661 tcgatgcgga gctggatgaa gacagtatcc gccccatttg atcgtaagca cagcttacgt
    12721 tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac
    12781 ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg
    12841 cagctggaag ttaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca
    12901 cataactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg
    12961 gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa
    13021 gaaaaaatgg cttctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc
    13081 tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg
    13141 ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg
    13201 ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt
    13261 gccgcacctt caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa
    13321 acggtcaaat ctgcgtggtg gagagtccgg cggacattta cgccattgag cagacggggc
    13381 agttttcagg tcgttatttt gtgttgatgg gacatctgtc accgctggac ggcatcggtc
    13441 cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag
    13501 tgatcctcgc caccaaccct acggttgaag gtgaagctac cgctaactac attgccgagc
    13561 tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg
    13621 aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc
    13681 gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc
    13741 ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg
    13801 aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc
    13861 cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat
    13921 gcggcggaca agttgcgttt ccgtgcgctc tctaacccgg acctgtacga aggtgatggc
    13981 gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctccgataac
    14041 ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt
    14101 accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt
    14161 cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact
    14221 cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa
    14281 tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt
    14341 gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc
    14401 gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt
    14461 atctcctggg agaaaattaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc
    14521 accgatgaag agtacaaaga gttctacaaa cacatcgccc acgattttaa cgatccgctg
    14581 acctggagcc acaaccgtgt tgaaggtaag caggagtaca ccagcctgct gtacattccg
    14641 tcccaggctc cgtgggatat gtggaaccgc gatcataaac acggcctgaa actgtatgtt
    14701 cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg
    14761 cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac
    14821 agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa
    14881 aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta
    14941 ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa attgctgcgt
    15001 tttgcttcta cccataccga ttcttctgcg cagaccgtgt ctctggaaga ctacgtttcc
    15061 cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta tgcggcagcg
    15121 aagagcagcc cgcacttgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc
    15181 gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag
    15241 tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg
    15301 aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag
    15361 cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc
    15421 gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg
    15481 ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca
    15541 gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcactg
    15601 ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg
    15661 ctggtttcct gatgtaatgc cggatgacct tcgtgtcatc cggcattttt cttttcatta
    15721 tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcccctggtg cctttcttga
    15781 ggcaatcgcc tgatggtggt atcgtttatc gctttttcaa aaaattcgac acattttaag
    15841 gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag
    15901 gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt
    15961 gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc
    16021 aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac
    16081 tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg
    16141 aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatc
    16201 gttgaccgta tcgtcggtcg ccgcgttcac gcgccgtctg gtcgtgttta tcacgttaaa
    16261 ttcaatccgc cgaaagtaga aggtaaagac gacgttaccg gtgaagaact gactacccgt
    16321 aaagacgatc aggaagaaac cgtacgtaaa cgtctggttg aataccatca gatgacagca
    16381 ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata cgcgaaagtt
    16441 gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca
    16501 agccgcagca gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac
    16561 agcaattagt tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag
    16621 aggcggtaat gcgtcagact aaaaccggta tcctgctggc aaacctgggt acgcccgatg
    16681 cccccacacc tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg
    16741 ttgatacctc acggttgtta tggtggccat tgctgcgcgg cgtgattttg ccgctgcgct
    16801 cgccgcgtgt ggcgaagctg tatgcctctg tctggatgga aggtggctcg ccgctgatgg
    16861 tttacagccg ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc
    16921 tgggaatgag ctacggctcg ccatcactgg aaagcgccgt agatgaactc ctggcagagc
    16981 atgtagatca tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg
    17041 cggtatggga tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt
    17101 ttatacgtga ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg
    17161 cttcttttgc caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc
    17221 agcgttatgc agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgtgaac
    17281 tggcttccgc attagggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg
    17341 gtcgggaacc ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag
    17401 gcgtaggtca tattcaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg
    17461 aagagattgc cgagcaaaac cgtgaggtct tcctcggtgc cggcgggaaa aaatatgaat
    17521 atattccggc gcttaatgcc acaccggaac atatcgaaat gatggctaat cttgttgccg
    17581 cgtatcgcta aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg
    17641 ttttcatcat ccgtgaataa tgcaaaaagg cgtgcagcgt gcctgggtag agtttgaact
    17701 cacagggctg ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg
    17761 gatcgaactc cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa
    17821 acagacagta atacggcgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca
    17881 tctgcaaatc ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg
    17941 aatcccgtaa tccgtaaagc ccataccaca gcaaaacgcc cgcaacttta ccgcaatcga
    18001 tctgtttatc acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac
    18061 cggcaaagcc aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac
    18121 aacaagcagc cacaatttcc tctatcactt gcggaaaacg cgcttcaggt gaaagggtgt
    18181 aatcaatacc aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt
    18241 gggtatcgag attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gttgccgggc
    18301 tatctggctg cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt
    18361 aagctttggt tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat
    18421 actgtcgttg ctcagcaatc gttcccgttg cgggccaggg cggtaaatcc ggctgaagag
    18481 tattcacaac ggtcttcatt tcagcagaaa taaggtccag aacaggtaat ttgttttccg
    18541 gcttcataca aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg
    18601 cgatcccgcc tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt
    18661 aacattcgcc gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta
    18721 aatccaaaca ttacttcccc gtaaatgcac gcgatccgct gcttcagcaa ttccagccag
    18781 aaaacgaaac cagcgccgcc tgggtagtgg gtatcgatca aacgctggtc gatattgaag
    18841 cgaaagtgga tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga
    18901 ttgaggatga tgtagccgaa gcgctttatc aggaactaaa acagaaaaac ctgattaccc
    18961 atcagtttgc gggtggcacc attggtaaca ccatgcataa ctactcggtg ctcgcggacg
    19021 accgttcggt gctgctgggc gtcatgtgca gcaatattga aattggcagc tatgcctatc
    19081 gttacctgtg taacacctcc agccgtaccg atcttaacta tctacaaggc gtggatggcc
    19141 cgattggtcg ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagcc
    19201 ctggccacat gaaccagctg cgggctgaaa gtattccgga agatgtgatt gccggagcct
    19261 cggctctggt tctcacctct tatctggtgc gttgcaagcc gggtgaaccc atgccggaag
    19321 caaccatgaa agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg
    19381 gaactaagtt tgtcattgcc gagaatccac agtggtggca gcaattcctc aaagaccacg
    19441 tctctatcct tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt
    19501 tgttggcatc tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa
    19561 tcggcttgta tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgt
    19621 tgctgccggg cgctatagcc gaattcaacc agtatgagtt tagccgcgcc atgcgccaca
    19681 aggattgtca gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg
    19741 aaaaaatcat gaacaccaac ggagcagggg atggcgcact ggcagcgttg ctgcatgaca
    19801 ttaccgccaa cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct
    19861 ggttaactta ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg
    19921 tactgaacca gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg
    19981 aagagtctta ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg
    20041 ccgcatctgg cattttgctc tcaatgcctg atgcgacgct ggcgcgtctt atcatgccta
    20101 catatttttc atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc
    20161 ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgctcgcc catcactact
    20221 tgattcgcac cacgttcggt gatatacgcc acttcatcgt cataatgggc gcgggcaata
    20281 atctcaatat ccggattttt cgcgcgggca gatgccacaa tctcacccgc ttcataaccg
    20341 ttggggatcg tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct
    20401 tcgttcgccg cattgcccaa tactgcgcgg accccgcgct ctcgcagctc atcaacacgg
    20461 gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc
    20521 aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc
    20581 tgcttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc
    20641 agatatttct ccagtagtgc gaacagtact gggttgagca taatcgacag gatcgcccct
    20701 gccagtacca ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc
    20761 aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatagttaa tgccgtacgt
    20821 tgggagtgac caaacagtcg caccaggaaa aacgcggcta acgacttacc aaacagaata
    20881 atcgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac
    20941 atcccgacgg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg
    21001 gcggcacggt gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcaccgagt
    21061 gcaaaggaga catcaaacag ctctaccgca ccaaaggcaa tccctaacgc cagcgccagc
    21121 accgacaggg taaacagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc
    21181 accagacggc gacctaccag catcataatg gcgataaacg cgatcacttt gccgatggtg
    21241 atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc
    21301 actgcgggca gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg
    21361 atggcgattt gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac
    21421 accacggtac tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag
    21481 cccagcacgg cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca
    21541 ccgggaatgg cgatggcctt taccgccatc aaatccttga gcgaaaagtg cagacccacg
    21601 ccaaacatca acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca
    21661 acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga
    21721 gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca
    21781 atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt
    21841 ccatttttgg ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga
    21901 tgttcatgaa taattattga attttgcaga aaaatggaat tagctgcaaa aaagcacgga
    21961 tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag
    22021 ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata
    22081 tggtcaacat ccccaatagt ggcaggaaag cacatatttt atagactaac tcgatgctgg
    22141 tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatgccg aaagcaaaac
    22201 cgaaaaagag tccagaaacc ataccgatac gtcctggaag cagctcctga gcgtagacca
    22261 gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa acccccgtcc
    22321 agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg atagagcccc
    22381 aaatcacata tttccgccca attttatccc ctacaggccc gccgatcacc gtacctgccg
    22441 caacggcaaa caggaaggca aacagatgaa gctgagcatt ctggatagat aatccgaatt
    22501 tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa tatttcgaga
    22561 aaatgaggat taacagaatg ctgaccgcca gtaccacttt gttgcgcggc agtggattga
    22621 taatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac caacggctga
    22681 tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca acgttgcctt
    22741 tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg ccaaagttgc
    22801 cgccgacctg aaagatagat tgtgccaggc catgccgccc gccggaagcc atacgggcca
    22861 cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc gccgccagca
    22921 gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag accacttaag gtaaagcaca
    22981 tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg accactggtt
    23041 gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc ataaatgtca
    23101 gagaaaattc tgactgaagc agaggataaa tcgccagaat cagcgattgg atcatgtcgt
    23161 tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg gccttggtcg
    23221 ttgacgcagc cgcgcccgcc acaggctggg tttgttcact cattgccata ggaaagtcac
    23281 tttttcaggg ttgcgatgtt aagaatgatc ttatttgtga ttattacccg actaacatac
    23341 ctgtatgcgc cgcctgaagg aagtctcaac gctgaataca gaatttctaa tctggatgca
    23401 gatttatctt caccggacgc agacttgtct atgatgtcgc gtcatactat ttttcaacac
    23461 gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg gcgttagcac
    23521 tgttaaccac atttacactg gcgagtgaag ctgctctggc gtatgagcag gataaaacct
    23581 acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc aatgaatatg
    23641 gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg
    23701 ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc gtgcccgagt
    23761 ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc tatgacgcga
    23821 tggcgatcgg taatcatgaa tttgataacc cgctcaccgt attacgccag caggaaaagt
    23881 gggcaaagtt cccgttactt tctgccaata tctaccagaa aagtaccggc gagcgcctgt
    23941 ttaaaccatg ggcgctgttt aagcgtcagg atctgaaaat tgccgttatt ggtctgacga
    24001 cggatgacac agcgaagtta ggcaatccag aaaacttcac cgatattgag ttccgtaagc
    24061 ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag ccagacatta
    24121 ttatcgcggc gacacatatg gggcattacg ataacggcga tcacggttct aacgcaccgg
    24181 gcgatgtgga gatggcacgc gcgttgcctg ccggatcgct ggcgatgatt gtcggcggtc
    24241 actcgcaaga tccagtctgt atggcagcgg aaaataaaaa gcaggtagat tacgttccag
    24301 gtacgccatg caaaccggat caacaaaacg gcatctggat tgtacaggcg catgagtggg
    24361 gcaagtacgt tggtcgggcc gactttgagt ttcgcaacgg cgaaatgaaa atggttaact
    24421 accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc
    24481 gcgtgctgta cacccctgaa atcgctgaaa accagcaaat gatctcgctg ttatcgccgt
    24541 tccagaacaa aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat ggtcgtctgg
    24601 aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg attctggcag
    24661 cacaaatgga tcgcactggt gccgactttg cggtgatgag cggaggcgga attcgtgatt
    24721 ctatcgaagc aggcgatatc agctataaaa acgtgctgaa agtgcagcca ttcggcaatg
    24781 tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc gccgtcgcgc
    24841 agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt gtggcgaaag
    24901 acggcaaact gaacgacctt aaaatcaaag gcgaaccggt cgatccagcg aaaacttacc
    24961 gtatggcgac attaaacttc aatgccaccg gcggtgatgg atatccgcgc cttgataaca
    25021 aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg tatatccaga
    25081 aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat
    25141 ccgaaagtgc cggatgttcg catccggcac aatgcttaat cgcggcgggc gatatcagca
    25201 aatttggcat cgaggatctt cgccagatcg cctgccgcca gttcgatatc cagtccgcgc
    25261 ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc
    25321 agacgttttt tctgccccag tgggctaatc cccccaacca ggtatcccgt cgaacgctgc
    25381 gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc tactttttta
    25441 aggtccagtt gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc
    25501 actgcgacca gcagcgtttt gtagacctga tccggattca aacctaattt tttgacgact
    25561 tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg aaacgaaatc
    25621 ttgttttttt cgagtgattt aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa
    25681 aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt
    25741 attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa agtgacggca
    25801 taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg ccattttttt
    25861 agcgcaacat ttgcggtaaa ttcccttctc catacaggtg tagtgcaccg accgcgacca
    25921 catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca
    25981 tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatttca ttattttgcg
    26041 gcggcgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt
    26101 gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc aacatggcaa
    26161 tctggttttc cgccccttcc agttcaatca cgggtttatg ttgttgcttc gccgcctgca
    26221 atagctgata atcgataccg tattctgccc gcagccccag tttttgtgcc tgcgtcgcct
    26281 gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa gagtgaggga gaaatgccca
    26341 tctcctggct aatgtgctgc agtttttgga gttgctcctc gctaatgcgc tcttccagcg
    26401 cctcgcaggc aggcaaatta gcaaaaggcg tatcgctggt ggaaacatcc gcctcgacga
    26461 tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggtagggga gccatatcgt
    26521 ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccggga agagagatat
    26581 caatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt
    26641 acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg ggagggatgg
    26701 cgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatgt tcttgtatcg
    26761 ctatttacag ggaagcaaca gttaaaaact atgaaacagg caacaagaaa accgacgaca
    26821 cccggagata ttcttctcta tgaatatctg gaaccgctag atttgaaaat caatgagtta
    26881 gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa
    26941 ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt cgatttttgg
    27001 ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg cacccaggaa
    27061 gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa
    27121 aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc gttcacgccg
    27181 catccggcat ccgagcacca ttgcctgatg cgacgcttac gcgtcttatc aggcctacaa
    27241 acctgtgcct gaaccgtagg ccggataagg cgtttacgcc gcatccggca gtcatgcgtc
    27301 gatgccaaat gcgccaccct aaagcagcgc atccgcaatg acgtacttat tccttcggtt
    27361 taaaccgcag caaccggttg gcgttactca ctacggtaat cgacgagagc gccattgccg
    27421 ctccggcaac taccgggtta agcagtgttc cagtaaacgg ccacaaaata ccggcggcga
    27481 ccggaatacc gatactgttg tagataaacg cgccgagcag gttctgcttc atgttgcgca
    27541 gcgttgcgcg ggaaatagcg agcgcatccg caacgcccat caggctatgg cgcatcaggg
    27601 taatcgccgc ggtttcaatg gcaacatcac tgccgccgcc cattgcaatg ccgacatccg
    27661 cctgggccag cgctggcgca tcgttaatgc cgtcgcccac cattgccacc tgacgtcctt
    27721 cactttgcag gcgtttaatc gcttcggctt taccgtccgg cagcaccccg gcgatcacct
    27781 catcaatccc tgcttctttg gcgatcgcat tggcggtggt tgggttatcc ccggtcaaca
    27841 tcaccagacg atatcccgct ttatgcagac gttgcagcgc cgccacgcta tcactacgca
    27901 acggatcgcg tacagccagc agggctaccg ctttcccgtc aaccgccagc agcacaggcg
    27961 ttgccccttg cgatgcctga gctgaaatat ctgcttcaat cgctttagta tcaacttgct
    28021 ggtcatttaa cagcgcctga ttgcccagca ataacgcatg accttcagct tcaccgctca
    28081 cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata tcgcttgctt
    28141 tatcgaggat cgcccgcgcc agcggatggc tggaaccttg ttccagtgcc gccgccagac
    28201 gcagcgcctg cgcttcgtca aaatcagcaa atgttttcac tgcgacaacc tgcggcttcc
    28261 cttcggtcag cgtcccggtt ttatcgaaca ctacggtatc gagtgtacta gcgcgttgca
    28321 gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc ccgcccgacg ccggaaataa
    28381 tcgacatcgg cgtcgccagc cccagcgcac acggacaggc aataatcagc accgtggtgg
    28441 caatcaccag ggtatagaca atctgcggtg ccggaccaaa gaaataccag attgccgcac
    28501 tgacaagcgc aataaccact actaccggca caaatacggc tgagatttta tccgccagct
    28561 gaccgatttc tggcttgctg ctctgggcct ggcgcaccat acgaataatc cttgatagcg
    28621 tggtatggct gccaaccgca ctggcgcgaa acagaacgct accgtcctgt accactgtcc
    28681 cggcatggac gctatcgcct tcgccttttt gctgcgggat aggttcgccc gtcagcatcg
    28741 cttcatccag ccatgcttcg ccctgggtaa tctcgccatc tactggcacg cggtcgcccg
    28801 tcgtcaggcg cagtagcatg cctggctgaa cttccgccag cggcacgctt ttttcacctt
    28861 cgtcagtaac caggcgcgcc gtcggcggag ttaaatcgag taatttctcc agcgcctttg
    28921 aagaacgctg acgtgcgcgc gcttccagca tatgaccgag attaatcaga ccgataatca
    28981 tcgcactggc ttcatagtaa agatgccgcg cttccatcgg gaaccactgc ggccacaggt
    29041 tgacgctcat cgaatagagc cacgccacac ccgtacccag cgccaccagc gtatccatcg
    29101 tcgccgcacc gttcagcagg cttttccatg cactgcggta aaaatggccg ccggcgaaaa
    29161 ccatcactgc cagggttatc aggccgataa ccaaccacag gctgcggttg tcagcggtga
    29221 ccatcatgtt atcgccgatc atcccccaga ccattaccgg gatacccaca gccagtgcaa
    29281 caattgcctg ccagcggaag cgcttcatcg tagcgacggc ggtttcttgc tggcgctcgc
    29341 ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc cgctttttcc accgcctgca
    29401 ctaaatcttg tggggaggca ctgcccatca ccagcgcagt acgctccgcc aggtttaccc
    29461 gtgcctgagt gacgcccggt acgctttgca gcgcattttg tacgcgggtg acacagctgg
    29521 cgcagctcat gccgctcagc agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg
    29581 gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat tgatgactcc gccagcggtt
    29641 tagcctttgg gtggcttaca gatgcgtcat aacccgcttg tttgatggtt tcgatcagct
    29701 gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat agacacatcc gcttgttcca
    29761 catccgggcg ctgttcgaga ctttctttca cgcgtttaac gcagtgaccg caggacaggc
    29821 cgtccagggt caggtcgata gtttgtgaca taaaacactc ctttaagaca gttttgactg
    29881 gctgtgataa aggttaaacc ttccagcaag gggaaggtca agaaattaat aacccaggcg
    29941 agtaaaagtc cgtaaagatt aaaaaatcgg ctcgatttgc atcaggatta gacatttatc
    30001 tctttgtttt cctgtagtta agttgcgggt gctaagttaa atcaggatgc ctgaaaatcg
    30061 gcaccggggt gaggaattac ctcccgcatc tataaaaagg agttaacaaa agatgttaga
    30121 tgcaaacaaa ttacagcagg cagtggatca ggcttacacc caatttcact cacttaacgg
    30181 cggacaaaat gccgattaca ttccctttct ggcgaatgtg cctggtcaac tggcagcagt
    30241 ggctatcgtg accagcgatg gcaacgtcta tagtgcgggt gacagtgatt accgctttgc
    30301 gctggaatcc atctcgaaag tctgtacgtt agcccttgcg ttagaagatg tcggcccgca
    30361 ggcggtacag gacaaaattg gcgctgaccc gaccggattg ccctttaact cagttatcgc
    30421 cttagagttg catggcggca aaccgctgtc gccactggta aatgctggcg ctattgccac
    30481 caccagcctg attaacgctg aaaatgccga acaacgctgg cagcgaattt tacatatcca
    30541 acagcaactg gctggtgagc tggtagcgct ctctgacgaa gtcaaccagt cggaacaaac
    30601 aaccaacttc cataaccggg ccattgcctg gctgctgtac tccgccggat atctctattg
    30661 tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc tccacgctca tcaatactgt
    30721 tgaactggca acgcttggcg cgacgctggc ggcgggtggt gtgaatccgt tgacgcataa
    30781 acgcgttctt caggccgaca acgtgccgta cattctggcc gaaatgatga tggaagggct
    30841 gtatggtcgc tccggtgact gggcgtatcg tgttggttta ccgggcaaaa gcggtgtagg
    30901 tggcggtatt ctggcggtcg tccctggagt gatgggaatt gccgcgttct caccaccgct
    30961 ggacgaagaa ggcaacagtg ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact
    31021 cggctataac gtgtttaagg gctgatcatg atgaacacgg aaggtaataa cggtaacaaa
    31081 cccctcggtc tatggaacgt cgtttccatc ggcattgggg caatggtggg ggcggggatc
    31141 ttcgcgctgc tggggcaggc tgcgttgcta atggaagcct cgacctgggt cgcctttgct
    31201 tttggcggta ttgtggcgat gttttccggt tatgcctatg cgcgtctggg ggcgagctat
    31261 cccagcaatg gcggcattat cgacttcttt cgtcgcggat taggcaacgg cgtcttttcg
    31321 ctggcgctct cgttactgta cctgttgacg ctggcggtga gcatcgccat ggtcgcccgt
    31381 gcttttggcg cttatgccgt gcagtttttg catgaaggca gccaggagga gcaccttatt
    31441 ttgctctacg cgttggggat cattgcggtg atgacgcttt tcaactcctt aagcaaccat
    31501 gcggtagggc ggctggaggt gatcctcgtc ggcattaaaa tgatgatcct gttattgctg
    31561 attattgccg gtgtctggtc gctgcaaccg gcgcatattt ccgtctctgc gccccccagc
    31621 tccggtgcgt tcttctcctg tatcgggata accttccttg cctatgcggg ctttggcatg
    31681 atggcgaacg cggcggataa agtgaaagat ccgcaggtca ttatgccacg ggcgtttctg
    31741 gtggcaattg gcgttaccac gttgctttat atctcgctgg cgctggtttt gcttagcgat
    31801 gtatcggcat tagagttaga aaaatatgcc gataccgccg tagcgcaggc tgcttctccg
    31861 ctgctcgggc atgtgggtta tgtgatcgtc gtcatcggcg ctttactggc gacggcttca
    31921 gccatcaacg cgaacctgtt cgccgtgttt aacatcatgg acaacatggg cagcgaacgc
    31981 gaactgccga agcgaatgaa taaatccctg tggcggcaga gtacctgggg caacatcatt
    32041 gtcgtggtgt tgattatgct gatgacggcg gcactaaatt taggctcact cgccagcgtt
    32101 gccagcgcca cctttttgat ttgctatctg gcggtgtttg tggtggcgat ccgcctgcgt
    32161 catgatattc acgcctcgtt gccgattctt atcgtaggta cgttggtgat gttgttggtg
    32221 atcgttggct ttatctacag cctgtggtcc cagggcagcc gtgcgttgat atggattatt
    32281 ggtgcaatct tactcagcct tattgtggca atggtcatga agcgcaataa aaccgtataa
    32341 cacctctctg cgcgcagtac tgcctgtact gcgcctttgt ctttaattca aattaccttt
    32401 cttattcctt taataaataa gtcacaaaaa aatgat
//
LOCUS       DAZFKY010000035        32382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFKY010000035 DAZFKY010000000
VERSION     DAZFKY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(313..2073)
                     /gene="ycaO"
                     /locus_tag="ACHKCL_001451"
     CDS             complement(313..2073)
                     /gene="ycaO"
                     /locus_tag="ACHKCL_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5762452.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2203..2895
                     /locus_tag="ACHKCL_001452"
     CDS             2203..2895
                     /locus_tag="ACHKCL_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5762453.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3094..4182
                     /gene="serC"
                     /locus_tag="ACHKCL_001453"
     CDS             3094..4182
                     /gene="serC"
                     /locus_tag="ACHKCL_001453"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5762454.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4253..5536
                     /gene="aroA"
                     /locus_tag="ACHKCL_001454"
     CDS             4253..5536
                     /gene="aroA"
                     /locus_tag="ACHKCL_001454"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5762455.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5705..6469
                     /gene="ycaL"
                     /locus_tag="ACHKCL_001455"
     CDS             5705..6469
                     /gene="ycaL"
                     /locus_tag="ACHKCL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5762456.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6642..7325
                     /gene="cmk"
                     /locus_tag="ACHKCL_001456"
     CDS             6642..7325
                     /gene="cmk"
                     /locus_tag="ACHKCL_001456"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5762457.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7436..9109
                     /gene="rpsA"
                     /locus_tag="ACHKCL_001457"
     CDS             7436..9109
                     /gene="rpsA"
                     /locus_tag="ACHKCL_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5762458.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9269..9553
                     /gene="ihfB"
                     /locus_tag="ACHKCL_001458"
     CDS             9269..9553
                     /gene="ihfB"
                     /locus_tag="ACHKCL_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5762459.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9760..12024
                     /locus_tag="ACHKCL_001459"
     CDS             9760..12024
                     /locus_tag="ACHKCL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5762460.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            12061..13809
                     /gene="msbA"
                     /locus_tag="ACHKCL_001460"
     CDS             12061..13809
                     /gene="msbA"
                     /locus_tag="ACHKCL_001460"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5762461.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13806..14792
                     /gene="lpxK"
                     /locus_tag="ACHKCL_001461"
     CDS             13806..14792
                     /gene="lpxK"
                     /locus_tag="ACHKCL_001461"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5762462.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14829..16061
                     /gene="ycaQ"
                     /locus_tag="ACHKCL_001462"
     CDS             14829..16061
                     /gene="ycaQ"
                     /locus_tag="ACHKCL_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5762463.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            16113..16295
                     /gene="ycaR"
                     /locus_tag="ACHKCL_001463"
     CDS             16113..16295
                     /gene="ycaR"
                     /locus_tag="ACHKCL_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5762464.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16292..17038
                     /gene="kdsB"
                     /locus_tag="ACHKCL_001464"
     CDS             16292..17038
                     /gene="kdsB"
                     /locus_tag="ACHKCL_001464"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5762465.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            17192..18085
                     /locus_tag="ACHKCL_001465"
     CDS             17192..18085
                     /locus_tag="ACHKCL_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5762466.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(18062..18841)
                     /gene="elyC"
                     /locus_tag="ACHKCL_001466"
     CDS             complement(18062..18841)
                     /gene="elyC"
                     /locus_tag="ACHKCL_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5762467.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18977..19762
                     /gene="cmoM"
                     /locus_tag="ACHKCL_001467"
     CDS             18977..19762
                     /gene="cmoM"
                     /locus_tag="ACHKCL_001467"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5762468.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19759..21081
                     /gene="mukF"
                     /locus_tag="ACHKCL_001468"
     CDS             19759..21081
                     /gene="mukF"
                     /locus_tag="ACHKCL_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5762469.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            21062..21766
                     /gene="mukE"
                     /locus_tag="ACHKCL_001469"
     CDS             21062..21766
                     /gene="mukE"
                     /locus_tag="ACHKCL_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5762470.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21766..26226
                     /gene="mukB"
                     /locus_tag="ACHKCL_001470"
     CDS             21766..26226
                     /gene="mukB"
                     /locus_tag="ACHKCL_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5762471.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26487..28334
                     /gene="ldtD"
                     /locus_tag="ACHKCL_001471"
     CDS             26487..28334
                     /gene="ldtD"
                     /locus_tag="ACHKCL_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5762472.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28515..29063
                     /locus_tag="ACHKCL_001472"
     CDS             28515..29063
                     /locus_tag="ACHKCL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5762473.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            29090..29737
                     /gene="gloC"
                     /locus_tag="ACHKCL_001473"
     CDS             29090..29737
                     /gene="gloC"
                     /locus_tag="ACHKCL_001473"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5762474.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29788..30978)
                     /gene="aspC"
                     /locus_tag="ACHKCL_001474"
     CDS             complement(29788..30978)
                     /gene="aspC"
                     /locus_tag="ACHKCL_001474"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5762475.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31163..32251)
                     /gene="ompF"
                     /locus_tag="ACHKCL_001475"
     CDS             complement(31163..32251)
                     /gene="ompF"
                     /locus_tag="ACHKCL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5762476.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7913 a         7867 c         8673 g         7929 t
ORIGIN      
        1 cccgtaactc gcatggtttt tatgcaaata cggagtaaat atttgattat ccaaataaaa
       61 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
      121 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
      181 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
      241 gggcattaaa aaaacgcccc gtaatataac tcagacgaat cattaagcct acattacgta
      301 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
      361 cttcaacagc gactgatgtg cagcaaacgc gtgcagatcg ctatctactg gttgcaagcc
      421 ataaaacgcc gcttcgccgc tcattgccgc actggcggct tccacggcat ctgcgccgta
      481 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gcgccagtaa
      541 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc gggctgaata ccgatgagtt
      601 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
      661 cagcatggct tttaattcac caatacgcag ggtgtaccaa ccgttatccg acccggtcgc
      721 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
      781 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
      841 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
      901 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
      961 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     1021 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     1081 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     1141 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     1201 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     1261 aaagcaggtg ccgttagcag gattaaacag taccacgcaa atcaccggat actggccgcc
     1321 aagggaacca tcatatgcga agatcgggaa accttctgct tccagtgttt cgatcgcttc
     1381 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     1441 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     1501 ttcgttacgg gtattgccag cagacatacc gttagaaacg tacaggttac caatgatatt
     1561 catcggaata taaacggtct gattatcaga ctgacgtgta aacggcagac cgcaaatacc
     1621 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     1681 atcgtaaaat gcgcgcagac gttcatcgag cagcccttct ggcacatcgt cattttcggt
     1741 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     1801 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     1861 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     1921 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     1981 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagtgc
     2041 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     2101 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     2161 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     2221 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     2281 ctttatacct ttgtactggt ctttttgttc ctcaaaatga ccggaagacg cggtgtgcgg
     2341 cagatgtcgc tgtttgaagt tttaatcatt ctgacgctgg gatcagcggc gggagatgtg
     2401 gcgttttatg atgatgtgcc gatggtcccg gtacttatcg tctttattac tctggcgttg
     2461 ttataccgcc tggtaatgtg gttgatggcg cacagtgaaa aactggaaga tcttctggaa
     2521 ggcaagccag ttgtcattat tgaagatggc gagctggcct ggtcgaaact caataactcc
     2581 aacatgacgg aatttgagtt ctttatggag ctacgattgc gtggcgtgga gcagctgggg
     2641 caggtacgtc tggcgattct cgaaaccaac gggcaaatca gtgtctattt ctttgaagat
     2701 gacaaggtga aatcgggttt acttatttta cccagtgatt gtacgcagcg ttacaaagtg
     2761 gtgccggagt cggcggacta tgcctgtatt cgttgtagtg aaatcattca tatgaaagcg
     2821 ggggaaaaac aattatgtcc gcgctgtgca aatccagaat ggacgaaggc aagtcgggca
     2881 aaacgggtga cctgacagta aaaacatcgg ctttttgcta ataatccgag agattctttt
     2941 gtgtgatgca agccacattt ttgccctcaa cggttttact cattgcgatg tgtgtcactg
     3001 aatgataaaa ccgatagcca caggaataat gtattacctg tggtcgcaat cgattgaccg
     3061 cgggttaata gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa ttttagttct
     3121 ggtccggcaa tgctaccggt agaggtgctt aaacaggctc aacaggaact gcgcgactgg
     3181 aacggtcttg gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt
     3241 gcagaggaag ccgagaagga ttttcgcgat cttcttaatg tcccctccaa ctacaaggta
     3301 ttattctgcc atggcggtgg tcgcggtcag tttgctgcgg taccgctgaa tattctcggt
     3361 gataaaacca ccgcagatta tgttgatgcc ggttactggg cggcaagtgc cattaaagaa
     3421 gcgaaaaaat actgcacgcc taatgtcttt gacgccaaag tgactgttga tggtctgcgc
     3481 gcggttaagc caatgcgtga atggcaactc tctgataatg ctgcttatat gcattactgc
     3541 ccgaatgaaa ccatcgacgg tatcgccatc gacgaaacgc cagacttcgg caaagatgtg
     3601 gtggtcgccg ccgacttctc ttcaaccatt ctttcccgtc cgattgacgt cagccgttat
     3661 ggtgtaattt acgctggcgc gcagaaaaat atcggcccgg caggcctgac aatcgtcatc
     3721 gtccgtgaag atttgctggg caaagcgaat gtcgcgtgtc cgtcgattct ggattattcc
     3781 atcctcaacg ataacggctc catgtttaac acgccgccga catttgcctg gtatctatct
     3841 ggtctggtct ttaaatggct gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat
     3901 cagcaaaaag cagaactgct atatggggtg attgataaca gcgatttcta ccgcaatgac
     3961 gtggcgaaag ctaaccgttc gcggatgaac gtgccgttcc agttggcgga cagtgcgctt
     4021 gacaaattgt tccttgaaga gtcttttgct gcaggccttc atgcactgaa aggtcaccgt
     4081 gtggtcggcg gaatgcgcgc gtctatttat aatgccatgc cgctggaagg cgttaaagcg
     4141 ctgacagact tcatggttga gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa
     4201 tccccacagc cagcctgtgg ggtttttatt tctgttgtag agagttgagt tcatggaatc
     4261 cctgacgtta caacccatcg ctcgtgtcga tggcactatt aatctgcccg gttccaagag
     4321 cgtttctaac cgcgctttat tgctggcggc attagcacac ggcaaaacag tattaaccaa
     4381 tctgctggat agcgatgacg tgcgccatat gctgaatgca ttaacagcgt taggggtaag
     4441 ctatacgctt tcagccgatc gtacacgttg cgaaattatc ggtaacggcg gtccattaca
     4501 cgcagaaggt gccctggagt tgttcctcgg taacgccgga acggcaatgc gtccgctggc
     4561 ggcagctctt tgtctgggta gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga
     4621 gcgcccgatt ggtcatctgg tggatgcgct gcgcctgggc ggggcgaaga tcacttacct
     4681 ggaacaagaa aattatccgc cgttgcgttt acagggcggc tttactggcg gcaacgttga
     4741 cgttgatggc tccgtttcca gccaattcct caccgcactg ttaatgactg cgcctcttgc
     4801 gccggaagat acggtgattc gtattaaagg cgatctggtt tctaaacctt atatcgacat
     4861 cacactcaat ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact atcaacaatt
     4921 tgtcgtaaaa ggcgggcagt cttatcagtc tccgggtact tatttggtcg aaggcgatgc
     4981 atcttcggct tcttactttc tggcagcagc agcaatcaaa ggcggcactg taaaagtgac
     5041 cggtattgga cgtaacagta tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat
     5101 gggcgcgacc atttgctggg gcgatgatta tatttcctgc acgcgtggtg aactgaacgc
     5161 tattgatatg gatatgaacc atattcctga tgcggcgatg accattgcta cggcggcgtt
     5221 atttgcaaaa ggcaccacca cgctgcgcaa tatctataac tggcgtgtta aagaaaccga
     5281 tcgcctgttt gcgatggcaa cagaactgcg taaagtcggt gcggaagtag aagaggggca
     5341 cgattacatt cgtatcactc caccggaaaa actgaatttt gccgagatcg cgacatacaa
     5401 tgatcaccgg atggcgatgt gtttctcgct ggtggcgttg tcagatacac cagtgacgat
     5461 tcttgatccc aaatgtacgg ccaaaacatt tccggattat ttcgagcagc tggcgcggat
     5521 tagccaggca gcctgaatga ataacgtcca ataaatagcc aaaactttct ttatcaaaac
     5581 gtcggcacat tgccggcgtt ttttttcgga ccttgtgagt cattttgatt aatggtagcg
     5641 tcgcttgtca atgtaagttg ttgatacata atgtttatat atgattaatc aacggatgat
     5701 tcacatgaag aatactaaat tactgctggc gattgtgacc tctgcagcat tactgacggg
     5761 gtgtcaaaat acccacggta ttgataccaa tatggctatc agctccggtt taaatgccta
     5821 taaagcagca acgttaagcg atgccgatgc aaaagcgatt gccaatcagg gctgtgccga
     5881 aatggacagc ggcaatcaag tcgcaagtaa atccagcaag tacggtaaac gtctggcaaa
     5941 aatcgccaaa gcattgggta acaatattaa tggcacgccg gtcaactata aggtttatat
     6001 gaccagcgac gtcaacgcat gggcgatggc gaacggctgt gttcgtgttt acagtggcct
     6061 gatggacatg atgaacgata acgaaattga aggcgttctg ggccatgaac tgggccacgt
     6121 cgcgttgggt cactcgctgg ctgaaatgaa agcttcttat gcgatcgttg ccgcacgcga
     6181 tgccatttca gctaccagcg gtgtggcttc ccagctttcc cgctcacaat tgggtgatat
     6241 cgcagaaggc gctatcaatg cgaaatactc ccgcgataaa gagtccgaag cagatgattt
     6301 ctcctttgat ctgttgaaga aacgtggcat cagcacccag gggctggttg gcagctttga
     6361 aaaactggct agcctggatg gcggtcgcac ccagtccatg tttgactctc acccaccatc
     6421 aacagagcgt gcgcaacaca tccgtgatcg tatcgcctct ggtaagtaaa tcattgtcat
     6481 ctttcgggct ggtcttctgc cagcccgcta taattgcgca ataaatcccc atctgaatac
     6541 agacaaaact ggtttttgca cacaacgtta acgatttgtg gcgtcggcgc gtataatgcg
     6601 cgcggttatg ttaacggtac gcctgtttta aggagataaa gatgacggca attgccccgg
     6661 ttattaccat tgatggccca agcggtgcag ggaaaggcac cttgtgtaag gctatggcgg
     6721 aagcgttgca atggcatctg ctggattcgg gtgcaattta tcgcgtactg gcactagcgg
     6781 cattacatca ccatgttgat gttgcatcgg aagatgcgct ggtgccgctg gcatcccatc
     6841 tggatgtacg ttttgtgtca accaatggca atctggaagt gatcctcgaa ggggaagatg
     6901 tcagcggcga aattcgtact caggaagtgg cgaatgcagc ttcacaagtc gcggcattcc
     6961 cacgcgttcg tgaagcatta ttgcgtcgcc aacgcgcgtt tcgcgaatta ccaggcctga
     7021 ttgccgatgg ccgcgacatg ggaacggtgg tattccctga tgcgccagtg aaaattttcc
     7081 ttgacgcctc ctcggaagaa cgtgcgcatc gccgcatgct acagttgcag gagaagggct
     7141 ttagtgttaa ctttgagcgc cttttggccg agatcaaaga acgcgacgac cgcgatcgta
     7201 accgagcggt agcgccactg gttccggcag ccgatgcttt agtgttggat tccaccacct
     7261 taagcattga gcaagtgatt gaaaaagcgc tacaatacgc gcgccagaaa ttggctctcg
     7321 cataagcgac cgaatttgca gtacccccgt tgcaatggaa tgacagcggg tatgttaaac
     7381 aaccccatcc ggcatggagc caggtggacg ttaaatataa acctgaagat taaacatgac
     7441 tgaatctttt gctcaactct ttgaagagtc cttaaaagaa atcgaaaccc gcccgggttc
     7501 tatcgttcgt ggcgttgttg ttgctatcga caaagacgta gtactggttg acgctggtct
     7561 gaaatctgag tccgccatcc cggctgagca gttcaaaaac gcccagggcg agctggaaat
     7621 ccaggtaggt gacgaagttg acgttgctct ggacgcagta gaagacggct tcggtgaaac
     7681 tctgctgtcc cgtgagaaag ctaaacgtca cgaagcctgg atcacgctgg aaaaagctta
     7741 cgaagatgct gaaactgtta ccggtgttat caacggcaaa gttaagggcg gcttcactgt
     7801 tgagctgaac ggtattcgtg cgttcctgcc aggttctctg gtagacgttc gtccggtgcg
     7861 tgacactctg cacctggaag gcaaagagct tgaattcaaa gtaatcaagc tggatcagaa
     7921 gcgcaacaac gttgttgttt ctcgtcgtgc cgttatcgaa tccgaaaaca gcgcagagcg
     7981 cgatcagctg ctggaaaacc tgcaggaagg catggaagtt aaaggtatcg ttaagaacct
     8041 cactgactac ggtgcattcg ttgatctggg cggcgttgac ggcctgctgc acatcactga
     8101 catggcctgg aaacgcgtta agcatccgag cgaaatcgtc aacgtgggcg acgaaatcac
     8161 tgttaaagtg ctgaagttcg accgcgaacg tacccgtgta tccctgggcc tgaaacagct
     8221 gggcgaagat ccgtgggtag ctatcgctaa acgttatccg gaaggtacca aactgactgg
     8281 tcgcgtgacc aacctgaccg actacggctg cttcgttgaa atcgaagaag gcgttgaagg
     8341 cctggtacac gtttccgaaa tggattggac caacaaaaac atccacccgt ccaaagttgt
     8401 taacgttggc gatgtagtgg aagttatggt tctggatatc gacgaagaac gtcgtcgtat
     8461 ctccctgggt ctgaaacagt gcaaagctaa cccgtggcag cagttcgcgg aaacccacaa
     8521 caagggcgac cgtgttgaag gtaaaatcaa gtctatcact gacttcggta tcttcatcgg
     8581 cctggacggc ggcatcgacg gcctggttca cctgtctgac atctcctgga acgttgcagg
     8641 cgaagaagca gttcgtgaat acaaaaaagg cgacgaaatc gctgcagttg ttctgcaggt
     8701 tgacgcagaa cgtgaacgta tctccctggg cgttaaacag ctcgcagaag atccgttcaa
     8761 caactgggtt gctctgaaca agaaaggcgc tatcgtaacc ggtaaagtaa ctgcagttga
     8821 cgctaaaggc gcaaccgtag aactggctga cggcgttgaa ggttacctgc gtgcttctga
     8881 agcatcccgt gaccgcgttg aagacgctac cctggttctg agcgttggcg acgaagttga
     8941 agctaaattc accggcgttg atcgtaaaaa ccgtgcaatc agcctgtctg ttcgtgcgaa
     9001 agacgaagct gacgagaaag atgcaatcgc aactgttaac aaacaggaag atgcaaactt
     9061 ctccaacaac gcaatggctg aagctttcaa agcagctaaa ggcgagtaat tctctgactc
     9121 ttcgggattt ttattctgaa gtttgttgag tttacttgac agattgcagg tttcgtcctg
     9181 taatcaagca ctaagggcgg ctacggccgc ccttaatcaa tgcagcaaca gcagccgctt
     9241 aatttgcctt taaggaaccg gaggaatcat gaccaagtca gaattgatag aaagacttgc
     9301 cacccagcaa tcgcacattc ccgccaagac ggttgaagat gcagtaaaag agatgctgga
     9361 gcatatggcc tcgactcttg cgcagggcga gcgtattgaa atccgcggtt tcggcagttt
     9421 ctctttgcac taccgcgcac cacgtaccgg acgtaatccg aagactggcg ataaagtaga
     9481 actggaagga aaatacgttc ctcactttaa acctggtaaa gaactgcgcg atcgcgccaa
     9541 tatttacggt taagttttta ctcaaacttg aacgagagaa aagcacctgt cgggtgcttt
     9601 tttcatttat ctaatctgga actggaagct gcctcgcaga gttttgagca gttttcaccc
     9661 tttcgttaaa ttcttctgaa tgtgcctcgg ggaacgcaaa attcccacca gacaaccgct
     9721 caacaaagtt gcacactttc cataaacagg gaggggtgca tgaaaataac gacagtcggc
     9781 gtatgcataa tttgcggaat ttttccgttg ctgattttgc cccaattgcc tgggacatta
     9841 acccttgcgt ttctgactct cttcgcctgc gtactggcat ttatccctgt taaaaccgtc
     9901 cgttatatcg cgctgacgtt gctgtttttc gtttggggca tattatcagc aaagcaaatt
     9961 ttgttggcag gagaaacctt aactggcgcg acgcaggatg caattgttga gatcactgct
    10021 actgacggca tgaccactca ttacggtcaa attacttatc tacaaggtca acgtatattc
    10081 cctgcgccag gccttgtgct gtatggcgaa tatcttccgc aagcggtttg tgccggacaa
    10141 ctgtggtcaa tgaaactcaa agttcgtgcc gttcatggtc aacttaatga tggcggcttt
    10201 gatagccagc gttatgccat tgcccagcat cagccgctca ccggccgctt tctgcaggca
    10261 agtgttattg aaccgaattg tagcctgcgt gcacagtatc tggcgtcact acaaacaacg
    10321 ctgcaaccct atccgtggaa tgcggttatt cttggtttag gtatggggga acggttatcc
    10381 gtccctaaag aaatcaaaaa tatcatgcgt gatactggaa cggcgcattt aatggcgata
    10441 tcgggattgc acatcgcttt tgcggcgttg ctggctgccg gactcattcg cagtggacaa
    10501 atttttctgc ctgggcgctg gatccactgg caaatgccat taattggcgg aatctgctgt
    10561 gctgcttttt atgcctggct gacgggaatg caacctcctg cattgcgtac cgttgtggcg
    10621 cttgctacgt ggggtatgct taagttaagt gggcgacagt ggagtggctg ggatgtatgg
    10681 atatgttgtc tggcggcaat tttgctgatg gatcctgttg tcattctctc gcaaagttta
    10741 tggctctctg ccgctgcggt cgcggcattg atattttggt atcagtggtt tccctgtcct
    10801 gagtggcaac tgccgccggt attgcgtgca gttgtttccc tcatccatct gcaactggga
    10861 atcacacttc tgcttatgcc cgtgcaaatc gtcatttttc atggcattag tctgacctcg
    10921 tttattgcaa atctattagc aattcccttg gtgacattta tcacggttcc gttgatcctc
    10981 gccgcgatgg ttgtgcattt aagcgggccg ttaatcctgg atcaagggtt atggtttctt
    11041 gccgaccggt ctttggcttt acttttcttg gggttaaaga gtttgccaga agggtggatc
    11101 aacattgctg aacgttggca atggctatca ttttccccat ggttcttact ggtggtatgg
    11161 cgattaaacg cctggcgaac gttgccagca atgtgtgtgg ctgtaggctt gctgatgtgc
    11221 tggccgctgt ggcaaaaacc tcgacctgac gagtggcaag tgtacatgct tgatgtcggg
    11281 caagggctgg caatggtgat agccagaaac ggcaaagcga ttctctatga cacgggactg
    11341 gcctggcctg aaggggatag tgggcaacaa ctgattatcc cctggctcca ctggcataat
    11401 cttgaaccgg aaggcgttat cctgagtcat gaacatctgg atcaccgggg agggctggac
    11461 tcaatattgc atacatggcc gatgttatgg atcagaagtc cgttaaactg ggaacatcat
    11521 cagccctgtg tgcgtggcga agcgtggcaa tggcaaggat tgcgtttcag cgcgcactgg
    11581 cctttacaag gtcgcaacga taaaggaaat aaccattcct gtgtggttaa ggttgatgac
    11641 gggacgaata gcattcttct aaccggtgat attgaagccc cagctgaaca aaagatgcta
    11701 agccgttact ggcagcaagt gcaggcaaca ttgcttcagg tacctcacca tggcagtaat
    11761 acctcatcat cattgccatt aattcagcga gtgaatggaa aagtggcact cgcatcggca
    11821 tcgcgctata acgcatggcg actgccctct aacaaagtta agcatcgcta tcaacaacaa
    11881 ggttatacgt ggcttgatac tcctcatcag gggcaagtaa cggtcgattt ttcagcgcaa
    11941 ggctggcgga ttagcagcct cagggagcaa attttacctc gttggtatca tcagtggttt
    12001 ggcgtgccag tggataacgg gtagaatatg cggctatttc aacaaatgct ggttttttga
    12061 atgcataacg acaaagatct ctctacgtgg cagacattcc gccgactgtg gccaaccatt
    12121 gcgcctttta aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc
    12181 gataccttca tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat
    12241 cgctccgtgc tggtgtggat gccgctggtg gtgatcgggc tgatgatttt gcgtggtatc
    12301 accagctatg tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgaccatg
    12361 cgtcgccgcc tgtttggtca catgatggga atgccggttt cattctttga caaacagtca
    12421 acgggtacgc tgttgtcacg tatcacctac gattccgaac aggttgcttc ttcctcttcc
    12481 ggcgcactga ttactgttgt gcgtgaaggt gcgtcgatca tcggcctgtt catcatgatg
    12541 ttctattaca gttggcaatt gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt
    12601 gcgattcgcg tagtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg
    12661 gggcaggtga ccaccagcgc tgaacaaatg ctgaaaggcc ataaagaagt attgattttc
    12721 ggtggtcagg aagtggaaac gaaacgcttc gataaagtca gcaaccgaat gcgtcttcag
    12781 gggatgaaaa tggtttcagc ctcttccatc tctgatccga tcattcagct gatcgcctct
    12841 ttggcgctgg cgtttgttct gtatgcggcg agcttcccaa gtgtcatgga tagcctgact
    12901 gccggtacga ttaccgttgt tttctcttca atgattgcac tgatgcgtcc gctgaaatcg
    12961 ctgactaacg ttaacgccca gttccagcgc ggtatggcgg cttgtcagac actgtttacc
    13021 attctggaca gtgagcagga gaaagacgaa ggtaagcgcg tgatcgagcg tgcgactggc
    13081 gacgtggaat tccgcaacgt cacctttact tatccgggac gtgacgtacc cgcattgcgt
    13141 aacatcaacc tgaaaattcc ggcagggaaa acggtcgctc tggttggacg ctctggttca
    13201 ggtaaatcaa ccatcgccag cctgatcacg cgtttttacg atattgatga aggcgaaatc
    13261 ctgatggatg gtcacgatct gcgcgagtat accctggcgt cgttgcgtaa ccaggttgct
    13321 ctggtgtcgc agaatgtcca tctgtttaac gatacggttg ctaacaacat tgcttacgca
    13381 cgaactgaac agtacagccg tgagcaaatt gaagaagcgg cgcgtatggc ctacgccatg
    13441 gacttcatca ataagatgga taacggtctc gatacagtga ttggtgaaaa cggcgtactg
    13501 ctctctggcg gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg
    13561 attctgattc tggatgaagc tacctcggct ctggataccg aatccgaacg tgcgattcag
    13621 gcggcactgg atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct
    13681 accattgaaa aggcagacga aatcgtggtc gtcgaggatg gtgtcattgt ggaacgcggt
    13741 acgcataacg atttgcttga gcaccgcggc gtttacgcgc aacttcacaa aatgcagttt
    13801 ggccaatgat cgaaaaaatc tggtctggtg aatccccttt gtggcggcta ttgctgccac
    13861 tctcctggtt gtatggcctg gtgagtggcg cgatccgtct ttgctataaa ctaaaactga
    13921 agcgcgcctg gcgtgccccc gtaccggttg tcgtggttgg taatctcacc gcaggcggca
    13981 acggaaaaac cccggtcgtt gtctggctgg tggaacagtt gcaacagcgc ggtattcgcg
    14041 tgggggtcgt atcgcgggga tatggtggta aggctgaatc ttatccgctg ttattgtcgg
    14101 cagataccac cacagcacag gcgggtgatg aacctgtgtt gatttatcaa cgcactgatg
    14161 cgcctgttgc ggtttctccc gttcgttctg atgcggtaaa agccattctg gcgcaacacc
    14221 ctgatgtgca gatcatcgta accgacgacg gtttacagca ttaccgtctg gcgcgtgatg
    14281 tggaaattgt cgttattgat ggtgtgcgtc gctttggcaa tggctggtgg ttgccggcgg
    14341 ggccaatgcg tgagcgagcg gggcgcttaa agtcagttga tgcggtaatc gtcaacggcg
    14401 gtgtcccccg cagcggtgaa atccccatgc atctgctgcc gggtcaggcg gtgaatttac
    14461 gtaccggtac gcgttgtgac gttgctcagc ttgaacatgt ggtggcgatg gcagggattg
    14521 ggcatccgcc gcgctttttt gccacgctga agatgtgcgg cgtacaaccg gaaaaatgtg
    14581 taccgctggc cgatcatcag tctttgaacc atgcggatgt cagcgcgttg gtaagcaccg
    14641 ggcaaacgct ggtaatgact gaaaaagatg cggtgaaatg ccgggccttt gcagaagaaa
    14701 attggtggta tttgcccgtt gacgcacagc tttcaggtga tgaaccagcg aaactgcttg
    14761 cgcaactaac ctcgctggct tctggcaact agttacgccg cggcagcgtt cgattgatgg
    14821 agtcatgaat gtcgctgccg cacctctccc ttgctgatgc gcgtaatctt caccttgccg
    14881 cgcaaggcct gttaaacaaa ccccgccgtc gagcgtcgtt ggaggatatt ccggcaacga
    14941 tctcccgcat gtccttgctg caaatcgata ccatcaatat tgttgcccgt agcccatatc
    15001 tggtgctttt cagtcgtctg ggaaattatc ctgcccagtg gctggatgag tctctggcgc
    15061 gtggcgaatt aatggaatac tgggcgcatg aagcctgctt tatgccacgc agtgactttc
    15121 gtcttattcg ccaccgcatg ctggcacctg aaaaaatggg ctggaaatac aaagacgcct
    15181 ggatgcagga acatgaggcg gaaattgcac agttaattca gcatattcat gataaggggc
    15241 cggtacgttc agccgatttt gagtatcctc gtaaaggtgc aagcggctgg tgggaatgga
    15301 agccgcataa acggcatctg gaaggtttat ttactgccgg aaaggtgatg gtgattgaac
    15361 ggcgcaactt ccagcgcgtt tatgatttaa cccaccgtgt catgcctgac tgggatgatg
    15421 agcgcgatct cgtttcgcaa acagaagcag aaatcatcat gctggataac agtgcgcgta
    15481 gccagggaat attccgcgaa cagtggctgg cagattacta tcggctgaaa cgtccggcac
    15541 tggcagcgtg gcgcgaagcg agggctgaac agcggcaaat cattgctgtg catgttgaaa
    15601 aattgggcaa tctttggctg catgctgatt tgctgccgct actcgagcga gcgctggccg
    15661 gaaagctcac tgcaacgcac agcgcggtac tttcgccttt tgatcctgtt gtctgggatc
    15721 gcaaacgcgc agagcagctt tttgatttta gctaccggct ggagtgctat accccagcgc
    15781 cgaaacgcca gtatggctat tttgttctgc cgttattaca tcgtgggcaa ttagttgggc
    15841 gaatggatgc caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac
    15901 aggaaggtat taaaccaacg acaacgctgc aaaaagggtt acgtcaggcg attactgatt
    15961 tcgctaactg gcagcaggca acgcgggtga cattaggaca ctgcccgcaa ggtctcttta
    16021 cggattgccg caccggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta
    16081 tgcattcata acccgatcca tctggaggaa ctatggatca tcgtctgctt gaaatcattg
    16141 cctgcccggt ttgcaacgga aaactttggt ataaccagga aaaacaagaa cttatttgca
    16201 aactggacaa cctggctttc ccgctgcgcg atggcattcc agtgttgctg gaaacggaag
    16261 cccgcgtgct gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg
    16321 ctacgcgtcc acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat
    16381 tgttcatgtt cttgaacgcg cgcgcgaatc aggtgccgag cgcatcatcg tggcaaccga
    16441 tcatgaggat gtcgcccgcg ccgttgaagc cgctggcggt gaagtatgta tgacgcgcgc
    16501 cgatcaccag tcagggacag aacgcttggc ggaagttgtc gaaaaatgcg cgttcagcga
    16561 cgacacggtg atcgttaatg tgcagggtga tgaaccgatg atccctgcga caatcattcg
    16621 tcaggttgct gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat
    16681 ccacaatgcg gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgctgaagg
    16741 gtatgcactg tacttctcac gcgctaccat tccgtgggat cgtgatcgtt ttgcaaaagg
    16801 ccttgaaacc gttggcgata acttcctgcg tcatcttggt atttatggct accgtgcagg
    16861 ctttatccgt cgttacgtca cctggcagcc aagcccgtta gaacacatcg aaatgttaga
    16921 gcagcttcgt gttttgtggt acggcgaaaa aatccatgtt gctgtcgcgc atgaagttcc
    16981 tggcacaggt gtggataccc ctgaagatct cgagcgcgtt cgcgctgaaa tgcgctaatt
    17041 tcacttcacg acacttcagc caattttggg aggagtgtcg taccgttacg attttcctca
    17101 atttttcttt tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat
    17161 gaaagcagta gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca
    17221 tttactgggc gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt
    17281 gtgggctttg tatgacagcc agggaaaccc aatgccgcta atggcaagaa gctttagtac
    17341 gcccggaaaa gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt
    17401 ccgtatgccg actatttatg gtgtgatgac gcatgaagaa caccccggcc ctgatgtcct
    17461 gctactggag cggatgcgtg gtgtttcggt agaggcacca gcccgaacac cagaacgttg
    17521 ggaacagctc aaagaccaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg
    17581 cggttgcgtc ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca
    17641 acatgttgaa gtgctatgga ccacgctcaa tcagttcaat aacaccggtc tgacgatgca
    17701 ggataagcgg atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggttttaa
    17761 cgacaattgt gtgctgattc acggtaactt ctgtttacgc agcatgttga aagattcgcg
    17821 cagcgatcag ttactggcga tggtcgggcc gggactaatg ctttgggcac cgcgagaata
    17881 cgaactgttc cgactaatgg ataattctct ggcggaagat ttgctctgga gttacctgca
    17941 acgcgcgcca gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga
    18001 agttgcgcaa ctggttaata ccggacgatt tagtcggcgc aacttcgatc tggcatcaaa
    18061 atcactcttg ccgtggctcg cctgacgaac cttttagcca ctgccagatg cggccaagcg
    18121 tttcatagcc aacacgatcg ctatgcatca accagaccgg agaagggatt gcccgttccc
    18181 acgggttaag cggcgagtcg atggccaact gattcgccgg ggctggtagt ggatttaacc
    18241 cttcctgctg aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga
    18301 aaggggcgtc accaatcgcc tgtttcactg ccgcagcttc ttcttcggta tcttttggca
    18361 aatccagggt gataatttgc tcgcgcggta cgcccagcga ttgcgcaact ctggcaccta
    18421 cttccgctgt actcaccgta ttggtttttg ctacgcctcc cgtgaagatc agttttgaac
    18481 ccggattttc gcgccataaa cggataccct cattcaggcg tggcagactg ttattgatta
    18541 aattagagct cggtgcccac tgcgggttcc aggtataacc acctccgagc accacgatat
    18601 agtccacttt ttgcgaatta ttccatgtcg gataggtgct ttcgatggga cgcagcaggc
    18661 ggtcggctac cggttgcaaa ctcaataaca agagtgccag ccacccaatg ctaataaaaa
    18721 tcttcccggt tttttgaaaa cggctaaacc acagtagcgc caggccagcg ccaataatta
    18781 acaacatcag cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca
    18841 tcctttctgg ttcctttttt aaccatatag caagggatcg ccagcgatat tacaccagac
    18901 gggttcattc tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca
    18961 accggtggag aaagcaatgc aggatcgcaa ttttgatgat attgcggaaa agttttcccg
    19021 taacatttac ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga
    19081 tcgcgtgctg gcggaaatgg gtccgcaaaa actgcgtgtg ctggatgcgg gcggtggaga
    19141 agggcagacc gcaatcaaaa tggccgagcg cgggcatcag gtcattttat gcgatctttc
    19201 tgcgcagatg atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat
    19261 gcaatttata cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct
    19321 gatattgttc catgcggtgc tcgagtgggt ggctgatccc cgcagcgtat tgcagaccct
    19381 gtggtcagtg ttgcgtccag gcggcgtgtt gtcgttaatg ttctacaatg cgcatggttt
    19441 gttgatgcat aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa
    19501 gaaaaaacgg acgctttcgc cagattatcc acgcgacccg acgcaggttt atctgtggct
    19561 ggaagaagct ggttggcaaa ttatgggtaa gacaggcgtt cgcgtgtttc atgattatct
    19621 gcgcgagaag caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta
    19681 ttgccgtcag gaaccgtata ttaccctggg gcgttatatt catgtcaccg cgcgcaaacc
    19741 gcagagcaag gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg
    19801 gccagaaaaa atgacttctc catttcgctg ccggtagacc gactctcttt tctgctggca
    19861 gttgccacgc tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat
    19921 gcattccgcc atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc
    19981 aacaacgcga tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag
    20041 gcagaaggga acgcaattta ccgtctgacg ccgctcggca tcggcattac tgactattac
    20101 atccgtcagc gcgagttttc tacgctgcgc ctttctatgc agttgtcgat tgtggcgggt
    20161 gagctcaaac gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt
    20221 aatgtctatg cgccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg
    20281 caacgtctga tggacgaaca gcagcagcag gtgaaggacg atatcgccca gttgctgaac
    20341 aaagactggc gggcggcgat ttccagctgt gaattgttgc tttcggaaac ttccggaacg
    20401 ctgcgtgaat tgcaggatac actggaagcg gcaggcgaca aattgcaggc taatctgttg
    20461 cgcattcagg atgcgacgat gactcatgac gatctgcatt tcgtcgatcg tctggtgttc
    20521 gatctgcaga gcaaactcga ccgtattatc agttggggcc agcaatccat cgatttgtgg
    20581 attggctacg accgccacgt acataaattt attcgtaccg cgatcgatat ggataaaaac
    20641 cgcgtctttg ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg
    20701 ctaagttatg ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggcactgcgc
    20761 gatgaagaag tgactgggga acttccgcca gatttggaat acgaagagtt taacgagatc
    20821 cgcgaacagc tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg
    20881 ccgctggatc ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac
    20941 tttgacgttg cgcgcattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat
    21001 ttcaccggac tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg
    21061 catgtcatcg acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa
    21121 tccgttattt ccggcgctgg acagcgcctt acgttcaggc cgccatattg gcctcgacga
    21181 actggataat catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg
    21241 ttataacgtt gagcttattc gtgcaccgga agggttcttc tatttacgtc cacgttccac
    21301 cacgctgatc cctcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg
    21361 ttatctctat ctcagcccgg aacggctggc gaatgagggg attttcaccc agcaggaact
    21421 gtacgacgaa ctgctcaccc tggccgatga agcgaaactg ctgaaactgg tgaacaaccg
    21481 ttcaaccggt tcagacattg accgtcagaa gttgcaggaa aaagtacgtt cttcgctcaa
    21541 ccgtctgcgt cgtttaggta tggtgtggtt tatgggccac gacagcagca agttccgcat
    21601 taccgaatcg gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc
    21661 acagcgtcgc ctgattcgtg atggcgaagc aatgccgatt gaaaatcatc tgcaactcaa
    21721 cgatgaaacc gaagagaatc agccagatag cggagaggaa gaataatgat tgaacgcggt
    21781 aaatttcgct cactgacgct gattaactgg aacggcttct ttgcccgaac ttttgacctt
    21841 gacgagctgg tcacgacgct ttctggcggt aacggggcgg gtaaatccac caccatggcg
    21901 gcgttcgtta cggcgctgat ccccgacctg accctgctgc atttccgtaa cactacggaa
    21961 gccggggcca ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc
    22021 tgttattcga tgctcgacac cattaactcg cgccaccagc gcgtggtggt cggtgtgcgt
    22081 ctgcaacagg ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcaggga
    22141 ctgccgatgt cggtgcagcc gacacagctg gtgaccgaaa ctctgaacga acgccaggcg
    22201 cgcgtgctgc cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt
    22261 aaacagttca actccattac cgattaccac tcgctgatgt tcgatctggg catcattgcg
    22321 cgtcgtctgc gctccgcatc tgaccgtagc aaattctatc gtctgatcga agcttcgctg
    22381 tatggcggga tctccagtgc cattacccgt tctctgcgcg actacctgtt gccggaaaac
    22441 agcggcgtgc gtaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg
    22501 ctggaagcga ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa
    22561 gctaccaact acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgtccatctc
    22621 gataaggccc tggagtttcg tcgcgaacta catacttcgc gtcagcaact ggcggcggag
    22681 cagtacaaac acgtcgatat ggcgcgtgag ctggcagagc acaacggtgc cgaaggcgat
    22741 ctggaagcgg attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcactgcgt
    22801 cagcaggaga agatcgaacg ctacgaagcg gatctcgatg agctgcagat ccgtctggaa
    22861 gagcaaaacg aagtggtggc agaagccatc gaacgccagg aagagaacga ggctcgcgcg
    22921 gaagctgccg aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg
    22981 ctggacgtcc agcaaacgcg cgcgatccag tacaaccagg cgattgctgc gcttaatcgt
    23041 gccaaagaac tgtgccatct gccggactta accgccgaca gcgccgccga atggctggaa
    23101 accttccagg cgaaagagct ggaagcgact gaaaaaatgc tctctcttga gcagaaaatg
    23161 agcatggcgc aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc
    23221 aacggcccgc tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg
    23281 gtcgatcagc gtcacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg
    23341 gaacagcgtc tgcgcgaaca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt
    23401 cagggcaaga attttgacat tgacgagctg gaagccctgc atcaggagct ggaagcacgc
    23461 attgcctctc tttccgatag cgtgtctaac gcccgtgaag agcggatggc actgcgccag
    23521 gagcaggaac agctgcagtc tcgcattcag agtttgatgc agcgtgcacc ggtttggctg
    23581 gcagcgcaaa acagtctcaa ccagttgagc gaacagtgcg gcgaagagtt cacctccagc
    23641 caggacgtca ctgaatatct gcaacagttg ctggagcgtg agcgcgaggc cattgttgaa
    23701 cgcgatgaag taggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag
    23761 cctggcggct ctgaagatca gcgtctgaac gcgctagcgg agcgttttgg tggtgtgctg
    23821 ctgtcagaaa tttatgacga cgttagcctg gaagatgcgc cgtacttctc agcgctgtat
    23881 ggcccgtcac gccacgccat cgtggtgcca gatctgtcac aggtaaccga acacctggaa
    23941 ggtttgaccg attgcccgga agatctctat ttgatcgaag gggatccgca gtcattcgat
    24001 gacagcgtgt tcagcgttga tgagctggaa aaagcggtag tggtgaaaat cgccgatcgt
    24061 cagtggcgtt attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc
    24121 cgtattgaaa gcctccatgc cgagcgtgaa gtgctttccg aacgcttcgc cacgctctcc
    24181 tttgatgtac agaaaaccca gcgtctgcat caggcgttca gccgctttat cggcagtcat
    24241 ctggcggtgg cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcgc
    24301 gtcgaactgg agcgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag
    24361 tttgagcagg cgaaagaagg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg
    24421 ttggctgatg acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc
    24481 caggaagccg cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc
    24541 gtttcggtat tgcagagcga cccggaacag ttcgaacagt taaaagaaga ttacgcgtac
    24601 tctcagcaga tgcagcgcga tgcccgtcag caggcgtttg ccctgacgga agtggtgcag
    24661 cgtcgtgcgc actttagcta ctctgactcg gcagaaatgc ttagcggtaa cagcgatctc
    24721 aacgaaaaac tgcgtgaacg tctggaacag gcggaagcgg agcgtacccg cgctcgcgaa
    24781 gcgttgcgcg gtcacgcagc gcagttgagt cagtacaacc aggtgctggc ttcgctgaaa
    24841 agttcttacg acaccaaaaa agagctactc aacgatctgc aacgtgaatt gcaggatatc
    24901 ggcgtgcgtg ctgatagcgg ggcagaagag cgggcacgta ttcgccgtga cgagctgcat
    24961 gcacaactga gcaataaccg ttcgcgccgc aatcaactgg aaaaagcgct caccttctgc
    25021 gaagcggaga tggataacct gacccgcaaa ctgcgcaagc tggagcgtga ttactttgag
    25081 atgcgcgagc aggtagtgac cgcgaaagcg ggctggtgtg cagtcatgcg catggtgaaa
    25141 gataacggcg ttgagcgccg cttacaccgt cgtgagctgg cttatctctc cgctgatgat
    25201 ttgcgttcca tgtcggataa ggcgttaggt gcgctgcgtc tggcggtggc ggataacgaa
    25261 catctgcgcg acgtgctgcg catgtcggaa gatcccaaac gtccggagcg taaaattcag
    25321 ttcttcgtgg cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc
    25381 gatgatccgg tggaagctat cgaacagatg gagatcgaac ttagccgtct gaccgaagag
    25441 ttaacctccc gtgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc
    25501 aaaaccattc agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta
    25561 tcgtttggtc aggtgaacag cgtgcgcctc aacgtgaacg tgcgtgaaac gcacgccatg
    25621 ctgctggatg tgctctctga acagcatgag cagcatcagg atctgtttaa cagcaaccgt
    25681 ctgaccttct cggaagcgct ggcgaaattg tatcagcgtc ttaatccgca gattgatatg
    25741 gggcagcgca cgccgcagac cattggcgaa gaactgctgg attaccgcaa ctatctggaa
    25801 atggaagttg aggttaaccg tggttccgat ggctggttgc gcgcagagtc tggtgcattg
    25861 tcgaccggtg aggcgattgg taccggtatg tcgattctgg tgatggtggt acaaagctgg
    25921 gaagatgaat ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc
    25981 gatgaagcag cgcgactgga tgctcgttct atcgccacgc tgtttgaatt gtgtgagcgt
    26041 ttgcaaatgc agctcatcat cgcagcgccg gaaaatatca gcccggagaa aggcaccacc
    26101 tataaactgg tgcgtaaagt cttccagaat accgaacatg ttcatgtcgt cggcctgcga
    26161 ggatttgcgc cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttctcaggcg
    26221 agttaaaatt aaggcggcag caatgccgcc ttttcttttt ccgaaaactc cgtttctgca
    26281 ctaaaaaagt ggcacattac ggcgcgcttt tctttaaact tctttacatt aggttatgta
    26341 aaaacgtatc ggcgtttata tactgaagat aagcctgatg agtaacaggc ttgctcgtca
    26401 tactttcgtg agtattggcg ttgtacaggc aagtcgtaaa ataacagcct ggctattcag
    26461 agtatgataa aaacaggggg caagggatgt tgcttaatat gatgtgtggt cgtcggctgt
    26521 cggcaatcag tttgtgcctg gccgtaacat tcgctccact gttcaatgcg caggccgatg
    26581 agcctgaagt aatccctggc gacagcccgg tggctgtcag tgaacagggc gaggcactgc
    26641 cgcaggcgca agccacggca ataatggcgg ggatcctgcc attgcctgaa ggtgcggcag
    26701 aaaaagcccg cacgcaaatc gaatctcaat tacccgcagg ttacaagccg gtttatctta
    26761 accagcttca actgttgtat gccgcacgcg atatgcaacc catgtgggaa aaccgtgatg
    26821 ctgttaaagc cttccagcaa cagctggcag aggtggcgat tgccggtttc cagccgcagt
    26881 ttaataaatg ggtagagtta ctgaccgatc ctggtgttaa cgggatggca cgcgacgtgg
    26941 tgctctctga tgcgatgatg ggctatctcc atttcattgc aaatattccg gtcaaaggca
    27001 ctcgctggct atatagcagt aaaccttatg cgcttgcaac gccgccgctt tcggtgatta
    27061 accaatggca gcaggcgctg gataaaggtc aattgcctac gtttgttgca ggactggcac
    27121 cgcagcatcc gcaatatgcg gtgatgcatg aatcgttact ggccttactc agtgacacca
    27181 aaccgtggcc ccaactgacc ggcaaagcaa cgttgcgccc agggcagtgg agtaacgacg
    27241 taccggcgtt gcgtgaaata ttgcaacgca caggcatgtt ggacgggggg ccgaaaatta
    27301 ctctacctgg cgatgacacg ccaactgacg cggtagtcag cccatccgct gttactgttg
    27361 aaacagcaga aactaagccg atggataagc aaacgacgtc tcgtagtaaa cctgcgcctg
    27421 ccgttcgcgc cgcctacgat aatgaactgg tggaagccgt taaacgtttt caggcatggc
    27481 aaggattggg ggcagatggt gctattggcc cggcaacgcg tgactggtta aacgtaacgc
    27541 ccgcccagcg tgctggcgtg ttggctctca acatccagcg attgcgcttg ctgccaacag
    27601 agctttctac cgggatcatg gttaacattc cggcctattc gctggtctac tatcagaacg
    27661 gcaatcaggt gctggattcg cgagtcattg tcggtcgccc cgatcgcaaa acgccgatga
    27721 tgagcagtgc ccttaacaat gtagtggtaa acccgccgtg gaacgtacct ccaactctgg
    27781 cacgcaaaga tattctgccg aaagtgcgca acgatccggg atatctcgaa agccatggct
    27841 atacggtgat gcgcggctgg aacagcagag aagcgattga cccatggcag gttgactggt
    27901 ctacaatcac ggcctcgaat ttaccgtttc gcttccaaca ggctccaggc ccacggaact
    27961 cgctggggcg ctataaattc aatatgccga gttcagaggc catttatttg catgacacgc
    28021 cgaaccacaa tctgttcaag cgtgatacac gcgcattgag ctcaggctgt gtacgagtga
    28081 ataaagcttc cgatctggcg aatatgctgt tgcaggatgc aggctggaat gacaaacgta
    28141 tttctgatgc gctgaagcag ggtgatacac gttacgtcaa tattcggcag tcgattccgg
    28201 tgaatctcta ctacctgacg gcctttgttg gtgcagatgg tcgtacccag tatcgtacag
    28261 atatttacaa ttatgatctg cctgcgcgat ccagctcgca aatcgtatcg aaagcggaac
    28321 aattaatcag gtaaatgaag tagttcgggg aaatcagttg tcgtaataat tagcatgatt
    28381 gggggcaatt ctctgcagcc cccgtcactg ctggggttga gtcatcttga cgtctgcttt
    28441 acgggcggtt aaggtgcctc ttgtgcgcca gaagtgcata taaacgataa cattgacctg
    28501 tagacttgat tatcatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg
    28561 gcgttgcact cggtgccgcc atcctgccga cccctgcgtt tgcaacactc tctaccccac
    28621 gcccgcgcat tttgacactc aataatcttc ataccggaga gtcaatcaaa gcggagtttt
    28681 tcgatggcag aggctatatt caggaagaat tggcaaaact taaccatttt ttccgcgatt
    28741 accgcgcgaa caaaataaag tccatcgacc caggattatt cgaccagttg tatcgcctgc
    28801 aagggttatt aggcacccgc aaaccggtgc aactcatttc cggttatcgt tccattgata
    28861 ccaacaatga actacgcgcc cgcagccgtg gagtagcgaa gaaaagctat cacactaaag
    28921 gccaggcgat ggatttccat attgaaggta tcgcgttaag caatattcgc aaagccgcgt
    28981 tatctatgcg cgcaggtggt gtaggatatt acccacgtag taactttgtg catattgata
    29041 ccgggccagc acggcactgg tagtaattgc ttaacgaaac aggggcagta tgaactatcg
    29101 tattattccg gtcaccgcat tctcccagaa ctgttcattg atctggtgtg aacaaacccg
    29161 cctggccgca ctggtcgatc ctggcggcga tgcggaaaaa atcaaacagg aagttgacgc
    29221 cagcggcctg acactgatgc agatcctgct gacgcatggt catctggacc acgttggcgc
    29281 agcggcggaa ctggcgcaac attacggcgt gccggttttt ggcccggaaa aagaagatga
    29341 gttctggctg caaggcttgc ctgcgcaaag tcgtatgttt ggtctggaag agtgccagcc
    29401 gctgacgcca gatcgttggc tgaacgaagg cgataccatc agcataggga atgtgacttt
    29461 acaggtgtta cattgccctg ggcatacgcc gggtcatgtc gtgttttttg atgatcgggc
    29521 aaagctgctg atttctggcg atgttatttt caaaggcgga gtagggcgca gtgacttccc
    29581 gcgtggcgat cataatcaac tgatttcttc aatcaaagat aaattgctgc cactgggtga
    29641 tgacgtgaca tttattccgg gtcacggacc attatccaca cttggttatg aacgcctgca
    29701 taatcccttc ctgcaagacg aaatgcccgt ctggtaaggc acacataaaa aagcccgctt
    29761 ttcagcgggc ttcattgttt ttaacgctta cagcactgcc acaatcgctt cgcacagcgg
    29821 agccatgtta tctggtgtca tcccggccac gtttacgcga ccagaagcaa cagcatacac
    29881 gccaaactct tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat
    29941 gccgttctgt ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt
    30001 gacgaacaac tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca
    30061 aatcgcacgt aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg
    30121 tgggttagag tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt
    30181 ttcactgtcg gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt
    30241 tttagagtag gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc
    30301 ttcagcatct tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa
    30361 ccagcctttc tcaacggaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat
    30421 accggttggg ttgtggcagc agccatggaa cagcactacg tcgccagcct gagcttcgtt
    30481 caggctgtta atcagtgcat cgaagtcaag ggtgtggttt tccgcatcat aataagcgta
    30541 ttcacgaact tccagatctg cagagttaaa gacgctctta tggttcggcc agcttgggtt
    30601 gctcacccac actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctatgcgtag
    30661 tgcgccagtg ccacccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct
    30721 acctttacca aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc
    30781 gaggtaattt ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt
    30841 cagtaccggg gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc
    30901 gggacgttca tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc
    30961 ggtaatgttc tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca
    31021 gggtaacggg agatttacaa aattccaact attactgatg aaaacgcagg ctgtttttgc
    31081 aagacgtgag attgctctgg aaggcataaa aaaaacagga ccaaagtcct gttttttcgg
    31141 catttaacaa agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt
    31201 ctgaacctac gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg
    31261 tggacatgtt tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa
    31321 catcaccgat accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca
    31381 gaccgaaatc gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc
    31441 cgctgatgtt tgtaaattta ttagtgatcg gcgtagcgtt acgcgtttca ccgtagttcg
    31501 ctgccaggta gatgttgttc gcatcatact tcagaccagt agcccactgt tcagcttttt
    31561 taccgttgcc aagaagttga gcttcttgca ggttggtacg gtcagctgca ccataagcac
    31621 caacgatacc aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag
    31681 agcggcgtgc agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat
    31741 caaccagacc aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga
    31801 agaagtcatc gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca
    31861 gtgcatcata aaccacaccg tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt
    31921 taagacccgc gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt
    31981 tgttaccctg gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgag
    32041 tttccccttt aaaaccaaga cgggcatagg tcatgtcgcc attgccaccg taactgtttt
    32101 caccgttacc cttggagaaa taatgcagac cgacagcttt accgtacaga tctactttgt
    32161 tgccatcttt gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga
    32221 cgatcactgc cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg
    32281 acacctgcca ctgccgtcaa taatttctgt caataaaatt ttacggaact attgatgaga
    32341 gtttggtgtc tttatgtgtc tgcaggcatc tttccattca aa
//
LOCUS       DAZFKY010000036        32332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFKY010000036 DAZFKY010000000
VERSION     DAZFKY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(2..77)
                     /locus_tag="ACHKCL_001476"
     tRNA            complement(2..77)
                     /locus_tag="ACHKCL_001476"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:His,seq:gtg)
     gene            complement(136..212)
                     /locus_tag="ACHKCL_001477"
     tRNA            complement(136..212)
                     /locus_tag="ACHKCL_001477"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Arg,seq:ccg)
     gene            complement(315..1700)
                     /gene="yifK"
                     /locus_tag="ACHKCL_001478"
     CDS             complement(315..1700)
                     /gene="yifK"
                     /locus_tag="ACHKCL_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5762477.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1891..2631)
                     /gene="wecG"
                     /locus_tag="ACHKCL_001479"
     CDS             complement(1891..2631)
                     /gene="wecG"
                     /locus_tag="ACHKCL_001479"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5762478.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2634..3986)
                     /gene="wzyE"
                     /locus_tag="ACHKCL_001480"
     CDS             complement(2634..3986)
                     /gene="wzyE"
                     /locus_tag="ACHKCL_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5762479.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3983..5062)
                     /gene="rffT"
                     /locus_tag="ACHKCL_001481"
                     /gene_synonym="wecF"
     CDS             complement(3983..5062)
                     /gene="rffT"
                     /locus_tag="ACHKCL_001481"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5762480.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCALGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5059..6309)
                     /gene="wzxE"
                     /locus_tag="ACHKCL_001482"
     CDS             complement(5059..6309)
                     /gene="wzxE"
                     /locus_tag="ACHKCL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5762481.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6311..7441)
                     /gene="rffA"
                     /locus_tag="ACHKCL_001483"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6311..7441)
                     /gene="rffA"
                     /locus_tag="ACHKCL_001483"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5762482.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7446..8120)
                     /gene="rffC"
                     /locus_tag="ACHKCL_001484"
                     /gene_synonym="wecD"
     CDS             complement(7446..8120)
                     /gene="rffC"
                     /locus_tag="ACHKCL_001484"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5762483.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8098..8979)
                     /gene="rfbA"
                     /locus_tag="ACHKCL_001485"
     CDS             complement(8098..8979)
                     /gene="rfbA"
                     /locus_tag="ACHKCL_001485"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5762484.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(8998..10065)
                     /gene="rffG"
                     /locus_tag="ACHKCL_001486"
     CDS             complement(8998..10065)
                     /gene="rffG"
                     /locus_tag="ACHKCL_001486"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5762485.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(10065..11327)
                     /gene="wecC"
                     /locus_tag="ACHKCL_001487"
     CDS             complement(10065..11327)
                     /gene="wecC"
                     /locus_tag="ACHKCL_001487"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5762486.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11324..12454)
                     /gene="wecB"
                     /locus_tag="ACHKCL_001488"
     CDS             complement(11324..12454)
                     /gene="wecB"
                     /locus_tag="ACHKCL_001488"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5762487.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12510..13556)
                     /gene="wzzE"
                     /locus_tag="ACHKCL_001489"
     CDS             complement(12510..13556)
                     /gene="wzzE"
                     /locus_tag="ACHKCL_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5762488.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13568..14671)
                     /gene="wecA"
                     /locus_tag="ACHKCL_001490"
     CDS             complement(13568..14671)
                     /gene="wecA"
                     /locus_tag="ACHKCL_001490"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5762489.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14911..16170)
                     /gene="rho"
                     /locus_tag="ACHKCL_001491"
     CDS             complement(14911..16170)
                     /gene="rho"
                     /locus_tag="ACHKCL_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5762490.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16255..16356)
                     /gene="rhoL"
                     /locus_tag="ACHKCL_001492"
     CDS             complement(16255..16356)
                     /gene="rhoL"
                     /locus_tag="ACHKCL_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5762491.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16497..16826)
                     /gene="trxA"
                     /locus_tag="ACHKCL_001493"
     CDS             complement(16497..16826)
                     /gene="trxA"
                     /locus_tag="ACHKCL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5762492.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16957..18222
                     /gene="rhlB"
                     /locus_tag="ACHKCL_001494"
     CDS             16957..18222
                     /gene="rhlB"
                     /locus_tag="ACHKCL_001494"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5762493.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGANGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18358..19842
                     /gene="gppA"
                     /locus_tag="ACHKCL_001495"
     CDS             18358..19842
                     /gene="gppA"
                     /locus_tag="ACHKCL_001495"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5762494.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19889..21910)
                     /gene="rep"
                     /locus_tag="ACHKCL_001496"
     CDS             complement(19889..21910)
                     /gene="rep"
                     /locus_tag="ACHKCL_001496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5762495.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22127..22576
                     /locus_tag="ACHKCL_001497"
     CDS             22127..22576
                     /locus_tag="ACHKCL_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO5762496.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22775..23056
                     /gene="ppiC"
                     /locus_tag="ACHKCL_001498"
     CDS             22775..23056
                     /gene="ppiC"
                     /locus_tag="ACHKCL_001498"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5762497.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23102..24577)
                     /gene="ilvC"
                     /locus_tag="ACHKCL_001499"
     CDS             complement(23102..24577)
                     /gene="ilvC"
                     /locus_tag="ACHKCL_001499"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5762498.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24727..25620
                     /gene="ilvY"
                     /locus_tag="ACHKCL_001500"
     CDS             24727..25620
                     /gene="ilvY"
                     /locus_tag="ACHKCL_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5762499.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25672..27216)
                     /gene="ilvA"
                     /locus_tag="ACHKCL_001501"
     CDS             complement(25672..27216)
                     /gene="ilvA"
                     /locus_tag="ACHKCL_001501"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5762500.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27219..29069)
                     /gene="ilvD"
                     /locus_tag="ACHKCL_001502"
     CDS             complement(27219..29069)
                     /gene="ilvD"
                     /locus_tag="ACHKCL_001502"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5762501.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29134..30063)
                     /gene="ilvE"
                     /locus_tag="ACHKCL_001503"
     CDS             complement(29134..30063)
                     /gene="ilvE"
                     /locus_tag="ACHKCL_001503"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5762502.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30083..30346)
                     /gene="ilvM"
                     /locus_tag="ACHKCL_001504"
     CDS             complement(30083..30346)
                     /gene="ilvM"
                     /locus_tag="ACHKCL_001504"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5762503.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30343..31989)
                     /gene="ilvG"
                     /locus_tag="ACHKCL_001505"
     CDS             complement(30343..31989)
                     /gene="ilvG"
                     /locus_tag="ACHKCL_001505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5762504.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31992..32042)
                     /gene="ilvX"
                     /locus_tag="ACHKCL_001506"
     CDS             complement(31992..32042)
                     /gene="ilvX"
                     /locus_tag="ACHKCL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5762505.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(32129..32227)
                     /gene="ilvL"
                     /locus_tag="ACHKCL_001507"
     CDS             complement(32129..32227)
                     /gene="ilvL"
                     /locus_tag="ACHKCL_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5762506.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         7804 a         8927 c         8195 g         7406 t
ORIGIN      
        1 atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc
       61 aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca
      121 agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag
      181 ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac
      241 ggacttcccc cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt
      301 gtgcgttttg ctgcttattc ctccagttta tgcgctttcc cgtggcgatt aaggccaaaa
      361 actttataaa tcgccgtcac cgccagcata aagatgatgc caacaaacag cgacatacgc
      421 gtatcttcat taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag
      481 ttggcccacg ggaacaggat tgagcggaac ggatggctgg caatcgccgc tttatgtgca
      541 cgccgaaaac gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg
      601 ctggcactgt agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat
      661 gagccaatta gcagaatagc aatagatact gccacacccg caaccggtac gccgtgacgg
      721 gaaactttcg ccatcgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt
      781 ccgcaactgt acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg
      841 ataatgcccg ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg
      901 ttgctgccta tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gccaacgtag
      961 aaaatcagga tccgccacag caccttgcct acggcactgc gcagcgtcac ctgcggattc
     1021 ttcgcttcac cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata
     1081 cacagcgcgg tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga
     1141 ttgctaaaac caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca
     1201 atgacaatca tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg
     1261 ccgtacaacc gcaccgctgc cagattcgcc aacgccacca gcgccactgc gatcaatgcg
     1321 ggtatccact gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt
     1381 tcagagatcc ccaccgccat ccacataaac cagtaagacc aggcggtgag atagccaaag
     1441 aacgggctca tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac
     1501 aacatttcgc ccattgaacg catgatgaaa aagacgaaca gcccggcgat gatataggcc
     1561 aacaatacgg atggcccggc ccatttcagg gtactggcgg cccccataaa caggccgacg
     1621 ccaatggtgc cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc
     1681 tctggtttgt tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg
     1741 gttatcgtta tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa
     1801 ataaagcatt aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacggagtga
     1861 ccactccgtc gctttacaaa gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa
     1921 ataacgcagc aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag
     1981 ccactccagc cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac
     2041 atcgtaagtc ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg
     2101 catgaagatc tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc
     2161 atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc
     2221 aacgatattc acattccact ggttgcgcag tttagcttca gtttgcgcca gcacttcagg
     2281 tttaccgccc acaagaaata ccgtcgtccc ttctttgcct gcgcgcgcca tcagctcttc
     2341 ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga
     2401 acgtacaacg ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac
     2461 ctcggcgttc tcttcaatgg tcagcatttt ttcagcatta atggcaacca gcgttccctg
     2521 cttaagctgc ccgtcagcaa acagataatc gagggcatgc tgcatatcac gccaaccaat
     2581 caactgtaag ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct
     2641 tcaacctgcg tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa
     2701 agccagtaca acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagacc
     2761 acgcgtgaga caaacgaatc cagcccttca cgcgccagca cgatcatatt gaagatcgcc
     2821 ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat tagtttcgcg gttgcccagc
     2881 tcatacagcc agtcgaacca tttgatgatc agtccaacca caatcgcccc gagcgggatg
     2941 aacaacgcgc cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg
     3001 gagtggttat tcagcacttc ccaggtaaag tagttggctg agttcagcac catactcggg
     3061 cgacccggcc acagccagga agggataaag acatagaaat cgcggacaat tggcgccagg
     3121 ccctggaagt cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgag
     3181 aaggtgtcgc gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt
     3241 ccatagcgtt ttagcgccag ccagaacatg ccaacaatcc ccagcacgcc cgccgccgcc
     3301 agcatccaca acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg
     3361 atgatgatat tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaggcgacc
     3421 gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc
     3481 atcgccggga tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggaa
     3541 aagatctgac tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg
     3601 ccgacgctta ccagcgcgat acccatcagg atcacccacg taagattggt ttccacgcgg
     3661 ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg
     3721 taggtgacgt agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaggatt
     3781 tctggcggcg cgacaccaac atcaaagcga aacaccagca cgctggtcag cgggaagcca
     3841 aagaaaaagg tgagcagaaa cagcaatgaa aagaatacat tgaagttaaa gcgcacacga
     3901 cgaaactcaa accaggtcag cgtggcaata aacagcgtgc agagcagcca gacaacaaac
     3961 aggccactga attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct
     4021 gccagccttg tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca
     4081 actggcgctg cgcttcacgc acaatatcct cgttgagatc gtcggtagta aacagcaccg
     4141 gtaaatgctg ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa
     4201 tgcccgcctg aatcagtaag cacagcgtac caatgccctg ctggcgggca aaaataaagt
     4261 aaccaagagc gcactgacga agtagcgtca gataggcgtc aaattccagt ttttcactca
     4321 gaacttgcag attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcgatgt
     4381 acgcttcgtt atgaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt
     4441 gctgatgaac ggcgcgcaag gcagcaatat gctcattgct gcggtcgccg gagttcccca
     4501 ccagaatggt cattttccct tcacgttgcc gatcgttcgc catcgtattg agagaagcat
     4561 ccatccgcgt cgggaagtac agcagttcgc cccgcacctt tgggtgcgtt ttggcaaaaa
     4621 agctcaaatc accacgggtg gcaaatacac agccgactcg cttttgcgcc aggcgacgta
     4681 gtgggtaaaa aagcttatat ctcaagccac tggaaagctc gtacaggtct gccccccaga
     4741 tatgccagta aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg
     4801 gattgaactg accgtggaag aaaaaacgct gctgacggtt agcttttgct ttcgcgatga
     4861 ccgcttccgc cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat
     4921 cacttaagcc gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg
     4981 ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca
     5041 gtacgtgaat cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag
     5101 agaaaaatag acgatatagg ttgccatata tgcctgtgcc gcgcccagcg cgccgtgcgt
     5161 agggattagc cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat
     5221 ataaaaccgc agtgaagctt tggcgatcac cagataacca aagacataag cgcccacttt
     5281 taacacatca cccaccaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga
     5341 caacagcagc cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac
     5401 tgccggtaag acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt
     5461 tagccgcgac aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta
     5521 ggcatcggaa atactgctca ccccttgcca gatccccacc tcatcccagc tatactgcgc
     5581 cgccagcagt ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgt
     5641 catgagcgta aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata
     5701 gcttaacggg atagcaccac gcttaattaa catgacggcg gcaggaatca ccaccagcgc
     5761 gggaatcaac gccagaccca gcaacgcccc ttcatatccg cccaaacggt aactgacgta
     5821 gtacgcaaga acgccaatca agctgccaac aatcagagat aacgcattac ctgcggcatc
     5881 gcgaaagcct ttcatcaacg ccagtaacag gttgccccag gcgatcccca tttgcaccag
     5941 cgccactaaa cgcaccagcc cctgatagtc gtgattacca aacaatccca agctgattgg
     6001 cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag aagccaagta ccatcgctga
     6061 tgaagtgccg accacgcggc gcagctgttg cggattatca tggtactggg caacgtattt
     6121 ggttacaccg ttaaagatgc cagccccggc aagcacgccg agcacggtaa tcaactggcg
     6181 gaaatttgcc gccagcccaa gccccgccgg accaaatgac accgccagca acttaccgac
     6241 cagtaacccg gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc
     6301 caacgacata tcaggagaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg
     6361 gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc
     6421 gatcttcacc gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt
     6481 aatggaacac cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcac
     6541 caatatcccg cagtttaatg taaaacatat gcgcgttctg caagcagcca tcgggaatcg
     6601 acggcagctc gatacgcccg gctttcgcca gaggtgctaa cgcatcgtag tagttttgcc
     6661 acagcgccag acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtacg
     6721 cagcttgcag atcggacatc aaatagctgg agccgatatc gcgccaggta tatttatcga
     6781 cctgaccacg gaagaactgg ctgcggtttg tgcctttttc acggatgatc tcggctcgtt
     6841 cgatcaacgc tttatcgtta atcagcgtcg cgccgccttc accgcccgcc gtgtagtttt
     6901 tggtttcatg gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt
     6961 aagtggacat cacgccctga gcagcatctt ccaccacaaa cagattatgc tttttcgcca
     7021 acgccataat ggtgtccatt tcgcaggcca cgcccgcgta atgcaccggc acgataacgc
     7081 gcgttttgtc ggtgatcgcc gcttcaatca acgtttcgtc gatgttcatg gtgtccgggc
     7141 gaacatccac aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg gtggagacaa
     7201 aggtgtagct cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca
     7261 tctccagcga agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct
     7321 ccagccactg ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca
     7381 ttgccgactg catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca
     7441 tgtgatcacc tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat
     7501 cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga
     7561 gcatacgccc agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc
     7621 aggccaattc gcgcatctgt agcattgagt tcccgtaaag agacataacc gcgaatatcg
     7681 ccggacgccg cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca
     7741 atccactgtg cataaaagcg actgctggcg tcaggcgcat accacggcgc acgaaaacgg
     7801 ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc
     7861 actacagcac cgctatcact ggcattgttc acgggtagcg ccaaatcaac ttcaccttct
     7921 accagggaga atcccagctg ttgcagggca tccagttccc ccgtatttga tgccgcaatt
     7981 ttggcctgca cccgtgacca cggcgctaac gcgtctggcg tcaggaacgg tgcttcagac
     8041 gtaatgcgca cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca
     8101 atactggcgc ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc
     8161 taatgaactg gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat
     8221 ctcttccagg caggcaatct taaagccctg gcgtttttcc accgtctgta caaaggtgct
     8281 ggcttcaatc aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag
     8341 ttcaacggtc aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc
     8401 accacgctcc gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata
     8461 aagcccggtc accgcccagt ttgatttcgg ctgttttggc ttttcttcca gcgagatagc
     8521 gcggaaatta tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca cctgatagcc
     8581 aaaaaccgtc gccacttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg
     8641 accgaagaag atgttatcgc ccagcaccag acaagaaggt tcaccattaa ggaaggtttc
     8701 accgatgata aaggcctgcg ccagaccgtc cgggctgggc tgttcggcat attccagctg
     8761 gataccgaac tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt
     8821 agtgatgatg agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat
     8881 cattggctta tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa
     8941 tcgggtaccg gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta
     9001 gcctttcaga cctaaacgct cgccctgata gctgccgtcc tgcacctgct tcctccagct
     9061 ttcattagcc agataccact gcaccgtttt acgcattcca ctttcaaagg tttcctgcgg
     9121 cagccagcca agttcacggg caattttcga agcatcaatg gcatagcgca gatcatgccc
     9181 cggacggtca gcgacaaagg tgatcaagtc acgataatgc accacgcctt gcggcttgtt
     9241 cggagccagt tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc
     9301 gttgtgacca ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag
     9361 cgcgcgggcg tgatcttcaa catacagcca gtcacggatt tgctgcccgt tgccatatac
     9421 cggtagcggt ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa
     9481 gtggtaagga ccgtagttat tcgagcagtt ggtgataagc gttggcagac cataggttcg
     9541 cagccaggcg cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg
     9601 cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc
     9661 gtcagtggag atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgcattcca
     9721 gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc
     9781 tgccgggccg tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc
     9841 tggctgatgc tcagtgaata cgcgcgccag ttctgcccga tcgcagatat caactttctc
     9901 aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt
     9961 cagcttatcg accactacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc
    10021 cgagccaata aacccggcac cgcctgttat cagaatcttt ctcatcagcg ccagactcct
    10081 ttagcatcga cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta
    10141 tgatcgacca gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga
    10201 gtacaaagcc cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagagtt
    10261 tcgccgctat gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg
    10321 tcatcaatat tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt
    10381 ttatcggtag ccgccaggca atcagccacc gccgctttca cctgatcgat aacccagaac
    10441 ggtttgtgat cgttcacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt
    10501 gccacgataa accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga
    10561 agaatattaa cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc
    10621 tgatcggcac aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg
    10681 ttttcggtga gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg
    10741 aggaaaattt tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc
    10801 acgcgatcgt ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag
    10861 taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag atccggacgc
    10921 atctctgcta accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg
    10981 atcaccagcg cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag
    11041 gtcatatctg gctcatgatc gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc
    11101 gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg
    11161 ctcgccaaat caggctcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt
    11221 tggttgatat cgacaccaat cacctgtttt tgccgtgagg caaacgctgc tgctgtaggc
    11281 agcccgatgt aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat
    11341 tattttttaa cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg
    11401 cgcggctcat agtttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa
    11461 ttcgctgctt atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct
    11521 cagtggtatc gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc
    11581 cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact
    11641 cctgaggatc gatcagaatg acatttttca catgccccag aatgcgattg accggctctc
    11701 tgacgttcgg gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga
    11761 tgtctgccag cgcgtggcag atttcttcaa agccacgacc gaagctttca cgcctgtgac
    11821 cggtcaccag aatcatcttt ttatcggggt cgataaacgg gtaatttgcc gcaagttctg
    11881 aacgcagcgt gtcgctgctc atcacctggt cacgcaccca taacagtgca tcaatgactg
    11941 tattaccggt aatgaagatt cggctatctg caacgttttc acgcagcaag ttttgccggg
    12001 aagtttcggt tggagagaaa tgatacatcg ccagatgccc ggtcaatgta cggttagcct
    12061 cttccggcca cggcgaatag agatcgcccg tgcgtaaacc agcctcaacg tgaccaacag
    12121 gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt cgtcgttgtg tcgccgtgaa
    12181 ccagcacgac gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac
    12241 aggttatctc tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa
    12301 tggaaaagag tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt
    12361 tagcctcaaa aaaaggatct tttgccaacg catgcaccaa cggagccatc ttgatggctt
    12421 ccgggcgcgt accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga
    12481 gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca
    12541 ccagccccga tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg
    12601 ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga
    12661 tccagggttg gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga
    12721 tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca
    12781 agcaggaaca tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa
    12841 atattatgct gctcagcaat tttcagcgcc tgctcaatgc tgttcatccg gcggtcgtag
    12901 atggctttcg ccacctcttc ctgacgcttc acctgtgctt tcatctggat ggtacgcgcc
    12961 gcccatgcgc ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca
    13021 acatactgac gtaacaggtt attagcgtca ggcgcggttt cggcaataag cttcacgctg
    13081 tcattgaccg cgcgggtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc
    13141 agcaacgccg catcggcttt gctgttgccc accatccgct gcttgtaata gtcggtttgc
    13201 agccagaact ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct
    13261 tcgtccatga ccgatggttg gtcggcagaa gccatgtttg aacggacatc caggttacgc
    13321 aaaaattgct gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc
    13381 gccgtcgagc tccactcctg acgagcaaaa aacgtatacg ccagcgcgat taacgcaaac
    13441 gccagcccca tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga
    13501 atatccagtt cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca
    13561 tcctcattta tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta
    13621 cgcgcttaat aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggagga
    13681 atgctagcaa aaagagcacc aacatgaccc actccgggac aaaatgagaa tattctgcca
    13741 gcacgccaat ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctggcggg
    13801 aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc
    13861 tcatgccttt acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga
    13921 ttatccacaa agcggtaacc gggctgatgg gatgggtttt gccttgggtc gtttcgagca
    13981 ggatccagat cacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt
    14041 agcggcgacc caggatacca aggttaagca tgatatatgg caggatggcg gcgatcatcg
    14101 caaagcacca gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg
    14161 caaacgagac gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg
    14221 cattaatggc cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca
    14281 tctcccagga gccaaagata taacccaggc tactgagata aaggttgcca aacaccatca
    14341 taacaatgcc aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt
    14401 catccagcgc gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg
    14461 gaatatagta atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatacccc
    14521 caacgagtgg tatcaacccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga
    14581 ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga
    14641 tgagatcagt actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc
    14701 agaagtataa ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagtc
    14761 ctgtgcaaaa attaaccatt tgatatgcaa attgcataac atgccagcaa attccaggaa
    14821 ttttcctaac atcgccagcg cggcattaag attacagata taaaagcaaa acgccacgta
    14881 aacacgtggc gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc
    14941 gtcattggtc ttggtcattg ccagtttatt aatgaggaat tccattgcat cgatttcgcc
    15001 catcgggtga atgattttgc gcaggatcca cattttctgc agttcttcct gagtcgtgag
    15061 cagctcttct ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc
    15121 agcgatctta cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtaaat
    15181 aacttcgtcc attttagaac cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc
    15241 gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgca gggcgttggc
    15301 atccacacca ccggtcaaca ctttacctga cgccggaaca acggtgttgt aagcgcgcgc
    15361 cagacgagtg atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt
    15421 cgccttctcg atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt
    15481 agaagcaaca acttcacctt ttaccagtcg ctgcatctcg gttacttctt ccggacgttc
    15541 gtcgatcagc agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat
    15601 gttctgcagc agcatggttt taccggcttt cggcggtgcc acaatcagac cacgctgacc
    15661 acgaccgata ggtgatgcca gatccagtac gcgagcggtt aaatcttcag tagaaccgtt
    15721 accacgttcc atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat
    15781 tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa
    15841 atagcgttca ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag
    15901 gttgaaacgg cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga
    15961 gctgtctgcg gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc
    16021 aaagatatct tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt
    16081 acgcatacga gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac
    16141 cggcgtattc tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa
    16201 tctcgaactt aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg
    16261 actcatgtct tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg
    16321 attgagtgac gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg
    16381 aaattacaag attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca
    16441 tgactcacgc cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac
    16501 gccaggttag cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt
    16561 gccgccactt caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc
    16621 ggcgcagtgc cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat
    16681 tcgtcagcga tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc
    16741 cagaaatcga cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca
    16801 gtcaggtgaa taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg
    16861 tttcgttgtt ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt
    16921 tcgtaaagca atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa
    16981 cagaagtttt ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg
    17041 tttcataact gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac
    17101 gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt
    17161 cattgtcttc tctctcatcc cgcgattgcc gatcgcaagg tgaatcagcc gcgtgcctta
    17221 attatggcac cgacgcgtga acttgccgtg cagatccatg ccgatgcgga accgctggcg
    17281 gaagctaccg gcctgaagct gggtctggct tacggtggtg atggttacga caaacagctg
    17341 aaagtgctgg aaagcggcgt tgacattctg attggcacca cggggcgttt aattgactac
    17401 gccaagcaga accacattaa cctcggtgcc attcaggtag tggtactgga cgaagccgat
    17461 cgcatgtacg atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct
    17521 gcaaaccagc gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg
    17581 gcgttcgagc agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc
    17641 caccgtatta aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa
    17701 acgctgatcg aagaagagtg gccagacaga gcgattattt tcgccaatac caaacaccgt
    17761 tgtgaagaga tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc
    17821 gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg cgatctggat
    17881 attctggttg ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt gacgcacgtc
    17941 tttaactacg atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt
    18001 cgcgcaggcg caaacggtca ctctattagc ctggcgtgtg aagagtatgc attgaatttg
    18061 cctgctattg agacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca
    18121 ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc cgcgcacagg caatggtccg
    18181 cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt aaataaaaaa tgtcatactc
    18241 aaaatcattc aaactgtatc aagatggcaa gagaatgaat cccgatgagc ttactgtagt
    18301 aagtgattca ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg
    18361 ggttccacct catcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg
    18421 gttgtgcgcg aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt
    18481 ctggctgctg gcctgaatag cgaaaatgcc ctctccaatg aagcaatgga gcgcggttgg
    18541 caatgtttgc gcctgtttgc tgaacgtctg caagatattc ctccctcgca aattcgcgtt
    18601 gtcgctacgg cgacgttacg ccttgccgtc aatgcgggtg attttattgc caaagcgcag
    18661 gaaatcctcg gttgtccggt acaggtgatc agcggtgaag aggaagcacg tctgatttat
    18721 cagggtgttg ctcacaccac tggcggtgcc gatcagcgcc tggtagtgga tatcggcggt
    18781 gccagtactg aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg
    18841 atgggctgcg tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat
    18901 tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg
    18961 tatcacggct ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc
    19021 atgatggcac aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag
    19081 cgagccattc attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt
    19141 gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt
    19201 cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat
    19261 ctggccgtcg agcaggatat tcgcagccgt acgctgcgta atattcaacg ccgctttatg
    19321 atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg
    19381 gaaaacgaat ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt
    19441 catgaaatcg gcctgagcgt tgacttcaaa caagcgccgc aacacgctgc ttatctggtg
    19501 cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa aattgctggc gacgctactg
    19561 ctcaaccaga ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccgccg
    19621 cgcgtcgcag aacaactctg ccgtttacta cgcctggcca tcatttttgc cagccgtcgc
    19681 cgtgacgatc tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg
    19741 cttccgcaag gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaagc
    19801 cagtggcaga gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc
    19861 gatgctgacg catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt
    19921 tcagattcgc cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc
    19981 gctcctgttc ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg
    20041 ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct
    20101 gggcgcgggt aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga
    20161 tactgctctg gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact
    20221 ccagtccttt tgacgcgtgg agagtcatca gttgcacctg atccagctct tcttcactct
    20281 caccacgctc catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg
    20341 gctcatccag ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt
    20401 tcttcatgcg catttcggcg gctttcgggc tgggcgatgt ttcgtacagc caggattcat
    20461 aatccatgcc atggatcaga tcacgcaccg cagcaatcgg ctcccgctcc gccaggcgct
    20521 ggatttctgc caaccagtga gtgaagcggg tcaatgcttc ataaccacgt ccgctaagcg
    20581 tctgactcag gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc
    20641 actcacccag ctttttcagc gtagccgggc caatctctcg cttcggcgtg ttaacgatac
    20701 gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct
    20761 tgatttcagg acgagagaaa aacgacgtac caccggatat tttgtacggg atgcggtttt
    20821 gcatcaggaa tttttcaaat acccgcgact gatggttgcc gcgataaaga atggcgtaat
    20881 ctttgtactg cgttttattg acgaagtgat gggcgatcag ctcgccagta acgcgctcgg
    20941 cttcatgttc ctcgttattc gcgcttaaaa cttttagctc tgtgccgtaa cccagttcgg
    21001 agaacagacg cttttcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca
    21061 gaatacgccc ggaagagcga tagttctgct caagcttaat caccttcagc gccggaaaat
    21121 cctgactcag cagcaccagg ttttgcggac gtgcaccgcg ccaggagtag atcgactggt
    21181 catcgtcacc caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat
    21241 actggctggt gttggtgtcc tgatactcat ccaccagcag gtagcgaatt ttgttctgcc
    21301 agcgttcgcg tacttcttca ttgcgttgca gcagcaacgt cggcaataaa atcaggtcat
    21361 cgaagtcgag aacgttacag gctttcaggt gtgcatcata cagcccataa caatgggcaa
    21421 aaatacgatc ccgctcgccc tttgcctctg ccgccgcctg cgccggagtt ttgagatcgt
    21481 tcttccagtt agagatggtc gaaatcagtt gttgcaggag aactttgtca tcttcaatca
    21541 gcccctcggt caactcttta agcaaagcaa gctgatcggt atcgtcaaat aacgagaagt
    21601 tcgctttcat cccaagcgcc gcatactcgc gtttgatgat atccagcccc aacgtatgga
    21661 aagtggagat catcagccca cgcgcctctt tacgccccag cgtctgccct acacgctctt
    21721 tcatctcgcg cgctgcttta ttagtaaagg tcaccgccgc aatgtgccgc gcctgataac
    21781 cgcaaccgcg gatcagatgg gcgattttat tggtgataac acgagtttta ccggaacccg
    21841 cgcccgccag caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt
    21901 ttagacgcat aggtgtattg ctcaatcttc gaacgggggg aggattgtag cagaatcgca
    21961 tttgaatgtg accgcgtgta agccgctaac cggttggctg cggatgttta aatcagagag
    22021 agaagaccga aatcggagtt catcgtgaat ccattagact tagaaaatat cgggtcgtaa
    22081 cgttgtgtta ttactttttc agagtttact aaggagaggt ttactcgtgg cgattaattt
    22141 cagccctaag gttggtgaaa tactggaatg caactttggt aactatcctg ttagccaaaa
    22201 tggaccgttc agcacgacgt attacgatgg ccgcattcct cctgaaatga taaaaaacag
    22261 gttagttgtc gtgctcaacg gaaaaattaa tggcaacgca ttcatcgtcg ttccattatc
    22321 aaccactcgc gaccatgaca agttaaaacg tggaatgcat gttgaaatag ccagtaatgt
    22381 tatcaatgac ctgcaattct ttgatcagca gattcgttgg gccaaaaccg acctggtaca
    22441 acaagtcagt cgaaacagat taaacagagc aagaacttac agagggtatc ttaatcaatg
    22501 tttgccacat gagttggttg cagacataca gagggcggtc atcaagtcaa tcaatgccat
    22561 atcgctcata aattgacaat aaaatcacca tccccgataa tgactttgta gcccatagag
    22621 gccctaagtt ttctcccctt gagggacctg cgaagcccgg attttccggg cttttgccat
    22681 atatgtacca gcccctccct gactgacaat tacgttagca tcggcggatc atgctaaaat
    22741 cgccgcctga caattattca ctcaaggcac gatcatggca aaaacagcag cagcactgca
    22801 tatccttgta aaagaagaga aactggctct ggatcttctc gagcagatta agaacggggc
    22861 cgattttggc aagctggcga agaaacactc catttgccca tcaggcaaac gcggcggtga
    22921 tttaggtgaa ttccgccagg gtcagatggt tccggcgttc gataaagttg tgttctcttg
    22981 tccggtactg gagccaaccg gcccgctgca cacccagttc ggttaccaca tcattaaggt
    23041 gctgtaccgt aactaatagc aaggccttct ccgggagaag gccttgaact ctgaatcgct
    23101 attaacccgc aacagcaata cgtttcatat cagtcatgta gccgcgcagt ttcttaccta
    23161 cctgctcaat cgcatggctg cgaatcgctt cgttcaaatc acgcagttgc gcgttatcta
    23221 ccgcaccttc cggaatagct ttacccaaat cgcccggttg cagctctgcc ataaacggtt
    23281 tcagcaacgg cacacaagcg taagagaaca gatagttacc gtactcagcg gtatcagaga
    23341 taaccacgtt catttcgtac agacgcttac gggcgatggt gttggcaatc agcggcagtt
    23401 cgtgcagtga ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc atggtttcaa
    23461 acgccagctc aacgcccgct ttcaccatcg cgatcatcag tacgccttta tcgaagtact
    23521 cctgctcgcc gattttgcct tcatactgcg gcgcggtttc aaacgcggtt ttgccggtct
    23581 cttcacgcca ggtcagcagt ttcttatcat cgttggccca gtccgccatc atgccggagg
    23641 agaattcgcc ggagatgatg tcgtccatat gtttctggaa cagcggtgcc atgatctctt
    23701 tcagctgttc tgacaacgcg taagcgcgca gtttcgccgg gttagagaga cggtccatca
    23761 tcagggtgat gccgccctgt ttcagcgctt cggtgatggt ttcccagccg aactgaatca
    23821 gtttttctgc gtatgccgga tcggtgcctt cttccaccag cttgtcgaag cacagcagag
    23881 agccggcctg caacataccg cacagaatgg tttgctcgcc catcaggtca gatttcactt
    23941 ccgcaacgaa agaggattcc agcacgcccg cacgatgacc accggttgcc gccgcccatg
    24001 ctttagcgat cgccatgcct tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg
    24061 tcggtacgcc gaatccacgt ttgtactctt cacgtacttc ggtgcctggg catttcggcg
    24121 caaccattac tacggtgatg tctttacgga tctgctcgcc cacttcgacg atgttgaaac
    24181 catgcgagta acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg tgcactacat
    24241 cagagtgctg cttgtccggc gtcaggttaa ccaccagatc cgcctgtggg atcagttctt
    24301 cgtaagtacc cactttaaaa ccattttcgg tcgctttacg ccaggacgcg cgcttctcgg
    24361 caatcgcttc tttacgcaga gcgtaggaga tatcgagacc agaatcacgc atgttcaggc
    24421 cctggttcag accctgtgcg ccacagccga cgatgaccac ttttttaccc tgaaggtagc
    24481 tcgcgccatc ggcgaattcg tcgcggccca taaagcgaca tttgcccaac tgtgccagtt
    24541 gctggcgcag gttcagtgta ttgaagtagt tagccatggt gattcctcgt gatgttgtgc
    24601 ttcttattgt tcggttcgcg tttgcaagat tgaattcact atatgacagg aaatttattg
    24661 cggaaattga tatattcaca acatcatatt gcaatttttg caacgtcaac atcgagggct
    24721 gtccctgtgg atttacgcga tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt
    24781 ggccgcagcg cgcgggcgat gcacgttagc ccatccacgc tctcacggca gattcagcgc
    24841 ctggaagaag atctcggtca gccgctgttt gtgcgcgata accgcacggt gacgctgact
    24901 gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc tgttgcagta tcagcagctg
    24961 cgccacacca tcgatcagca agggccgtcg ctctctggcg aattacatat cttctgctcg
    25021 gtgaccgctg cctacagcca tctgccgccg attctggatc gcttccgcgc ggaacacccg
    25081 tcggtggaga ttaaactcac tactggtgat gcggcagatg caatggaaaa agtggtcacg
    25141 ggagaagcgg atctggcgat tgccgggaaa ccggagacgc tacccggcgc tgtagcgttt
    25201 tcgatgctgg agaatctggc ggtagtgctg attgcgcccg cgctgccctg cccggtgcgt
    25261 aatcaggtct cggtagagaa gccggactgg tcaacggtac cgtttatcat ggccgatcag
    25321 gggccagtac gccgccgcat tgaactgtgg tttcgacgca ataaaatcag caacccaatg
    25381 atttacgcca cggttggcgg gcatgaagcg atggtatcga tggtggcgct cggctgtggt
    25441 gtggcgctgt tgccggaagt ggtgctggaa aacagcccgg agcctgtacg caaccgggta
    25501 atgattttag atcgcagcga tgagaaaacg ccgtttgaac tgggcgtgtg tgcacaaaaa
    25561 aagcggctgc atgagccgct gattgaggcg ttctggagga ttttgccgaa ccacaaatga
    25621 cgttgtcgcg cgagtaggcc tgataagcga agcgctatca ggcatttttc cctaacccgc
    25681 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc ccagctcatt
    25741 cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc
    25801 gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg tacccagcgt
    25861 gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt agaggcgttc
    25921 ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact tcgccatttc
    25981 gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca aaatttcttt
    26041 gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt ttttggcatc
    26101 ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc agaatttgag
    26161 gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct gttcgcccag
    26221 ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac cggaaagaat
    26281 atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca ttcccgccag
    26341 cgccagcgcg ccagagggtt ccgccaccgc gcgcacatct tcgaataaat ccttcatcgc
    26401 cgcacagatc gcatcactat cgacggtgat gatatcgtcg agatactcct ggcacaaacg
    26461 gaaggtttcg tcgccgatgc gttttaccgc aacgccttca gcaaacagcc ccacgcgcgg
    26521 cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt cttccgcttc
    26581 tacggcgatt actttgattt gcggcatcag ttgtttgatc agcaccgcca cgccagcagc
    26641 cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct gctggagcag
    26701 ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg gcggcaccca
    26761 ggtgaacccc tgctgctgtg acagttcgat cgctttggct ttcgcttcat caaagttcgc
    26821 gccgtggagc agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga tgtcggcggt
    26881 ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa acgcgacgcc
    26941 ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt cttccgtcag
    27001 gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct ggcgatcttc
    27061 gcgcttcacc agaatcacgt tatcaagacg agacgagagc ttttccattt tttgtagcgg
    27121 cgtgacctgc gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat attcagcacc
    27181 ttccggggca ccggacaggg gttgcgagtc agccattatt aaccccccag tttcgattta
    27241 tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg cataagcacg cagggcaaag
    27301 gagatctgac gttcacgatt tttcggcgtc caggctttgt caccccgagc ttcctgcgct
    27361 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcggaatg
    27421 tcgatggcga tcaggtcgcc gtcttcaatc aggccaatgc tgccgccgct tgccgcttcc
    27481 ggtgagacgt ggccgataga aaggccagag gtgccgccag agaaacgacc gtcggtgatc
    27541 agcgcacagg ctttaccgag ccccattgat ttcaggaagc tggttgggta gagcatttcc
    27601 tgcatccccg gaccaccttt tgggccttca tagcgaatta ctaccacatc tccggcgaca
    27661 actttaccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtacac tttcgccggg
    27721 ccggtgaatt tgaggatgct gtcatcgacg cccgcagttt taacgatgca gccgttttcc
    27781 gcgaaattac cgtagagcac cgccaggccg ccgtctttgc tataggcgtg ttccagcgag
    27841 cggatacagc cattggcgcg atcgtcgtcc agcgtatccc aacggcaatc ttgcgagaat
    27901 gcctgggtgg tacggatacc cgcagggccg gcgcggaaca tattttttac cgcgtcatcc
    27961 tgggtcagca taacgtcgta ttgttccagc gtttgcggca acgtcaggcc aagtacgttt
    28021 ttcacatcac ggttcagtaa ccccgcacga tccagctcgc cgagaatacc gataacacca
    28081 ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcgac tttgcataac
    28141 tgcggaacct tgcgggaaag cttatcgata tcactcatgg tgaagtcgat ttccgcttcc
    28201 tgcgccgccg ccagcaggtg aagtacggtg ttagtcgatc cacccatcgc gatatccagc
    28261 gtcatggcat tttcaaacgc cgctttgcta gcaatattgc ggggcagtgc actttcgtcg
    28321 ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccagcatt gaggaacagc
    28381 tgcttacggt cagagtgggt tgccagcagc gagccgttgc ccggctgcga cagacccagc
    28441 gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga accgcaggtt
    28501 gggcatgcgg aacgttcaac ctgatcgctc tgggagtcag atactttcgg gtctgcgccc
    28561 tggatcatcg catcaaccag atcgagcttg atgatctgat cggacagttt ggttttcccg
    28621 gcctccatcg ggccgccgga aacaaagatc accggaatat tcagacgcag ggaagccatc
    28681 agcatccccg gggtgatttt gtcacagtta gagatgcaga ccatggcgtc ggcgcagtgg
    28741 gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggcag tgaataaagc
    28801 atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa ctctttggca
    28861 acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatggacg
    28921 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt accgaaatcg
    28981 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    29041 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    29101 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    29161 ccatttatct tcggtttcgc cggtaaagag gccgaagaag gcttgctgaa tgcgtttggt
    29221 gaccgggcca caacggcctt cgccaacctg aataccgtct acactgcgca ctggcgtgat
    29281 ttctgccgcc gtaccggaca taaagacttc atccgccagg tacaaggatt cgcgcgacag
    29341 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt
    29401 aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat ctttcacttc
    29461 aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg cgataccttc
    29521 ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat agttaccacc
    29581 ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg aaaccatcgc
    29641 atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga aagcggcgat
    29701 aatcacatcg gttgagtatc ccgctggcgg gtttacgccc atgccaacat caccaacgaa
    29761 gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cacggcaagc
    29821 ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga tagattttgg cggagtcatg
    29881 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg agtcgtagca
    29941 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    30001 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    30061 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    30121 agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    30181 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcggcca
    30241 tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    30301 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    30361 catttctgaa ttactggcac caggcggcac cagcggccag acgttctcaa gttcgtcgat
    30421 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    30481 ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta acttgaggaa
    30541 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tggaaaaaca gttgctgcca
    30601 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    30661 gcgttttacg gtgcccagct cttgtacatt catcatgaaa gagccgtcac cggagataca
    30721 gacgacggta tcgtttggtc gcgcgacttg cgcgccaacc gccgccggta aaccaaaacc
    30781 catggtgcct aagccgctgg aggtaatgaa attttccggg cgagtgtgtg cgatgtgctg
    30841 cgcggcccac atctgatgct gccccacatc tgtggtcaca acgcaatccg caggtttacg
    30901 atccgacagt tgttttaaca acaacggcgc gtagatagcg tcaccgggat ggtcgtaacg
    30961 ccaggcatgt tcatcacgca gctgcgcgca gtattgctgc cagtcattga tatttaatgg
    31021 ctgctgtaat gctggtaaca gcgcatttaa atcaccctgt aatgccacat gtgcctgacg
    31081 cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt gtggtgcgaa
    31141 ggtgttcaat ttgccggtca cccggtcatc aaaacgtgca cccacggcga tcagcaagtc
    31201 gcactcctgc accgcgaagt tcgccgcttt ggtgccatgc attcccagca tgcccagata
    31261 gtacggataa tctgcttcaa ctgcgcccag ccctttcagc gtgcaggtgg caggcatttt
    31321 tgtggtagcg agaaattctc gtaaagcagg aactgcctgc gccataccca cgccaccacc
    31381 aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct caacttcggc
    31441 atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccaggt cgccgctggc
    31501 taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg agctggcaac
    31561 gtcgaatgct tcagccataa tgcgcggcaa ctcttccagc gactgcacca gaaagctgtg
    31621 cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt cagtaccgat
    31681 aaacggtgcg gacacttgac cggtgatggc aacaacaggg atagaatcta acagtgcgtc
    31741 cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac atacgccagt
    31801 tttgccggta gcacgggcat aaccgatagc cgccattgcc gcaccctgct catgtcggca
    31861 cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    31921 cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca cccactgtgc
    31981 gccattcata gttagttccc cgtcctgaat cttgggaaac agaattttgt gctgttattc
    32041 attgtctgct cctcggttat gtttttaagg tcaaaaaaaa cccccggacc tttcggtgcg
    32101 ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt gcagccccgc
    32161 acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact cgtagaaggg
    32221 ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt taccatagat
    32281 tttgcgaata gcacaagata ttttttggcc gttttttatg atggctttaa ta
//
LOCUS       DAZFKY010000037        31995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFKY010000037 DAZFKY010000000
VERSION     DAZFKY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHKCL_001508"
     CDS             complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHKCL_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5762507.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHKCL_001509"
     CDS             complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHKCL_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5762508.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1749..2618
                     /gene="pldA"
                     /locus_tag="ACHKCL_001510"
     CDS             1749..2618
                     /gene="pldA"
                     /locus_tag="ACHKCL_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5762509.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2751..4580
                     /gene="recQ"
                     /locus_tag="ACHKCL_001511"
     CDS             2751..4580
                     /gene="recQ"
                     /locus_tag="ACHKCL_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5762510.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4644..5264
                     /gene="rhtC"
                     /locus_tag="ACHKCL_001512"
     CDS             4644..5264
                     /gene="rhtC"
                     /locus_tag="ACHKCL_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5762511.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5326..5946)
                     /gene="rhtB"
                     /locus_tag="ACHKCL_001513"
     CDS             complement(5326..5946)
                     /gene="rhtB"
                     /locus_tag="ACHKCL_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5762512.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6057..7079
                     /gene="pldB"
                     /locus_tag="ACHKCL_001514"
     CDS             6057..7079
                     /gene="pldB"
                     /locus_tag="ACHKCL_001514"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5762513.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7087..7887
                     /gene="yigL"
                     /locus_tag="ACHKCL_001515"
     CDS             7087..7887
                     /gene="yigL"
                     /locus_tag="ACHKCL_001515"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5762514.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKEPLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7963..8862
                     /gene="bioP"
                     /locus_tag="ACHKCL_001516"
     CDS             7963..8862
                     /gene="bioP"
                     /locus_tag="ACHKCL_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5762515.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8750..9703)
                     /gene="metR"
                     /locus_tag="ACHKCL_001517"
     CDS             complement(8750..9703)
                     /gene="metR"
                     /locus_tag="ACHKCL_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5762516.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9939..12200
                     /gene="metE"
                     /locus_tag="ACHKCL_001518"
     CDS             9939..12200
                     /gene="metE"
                     /locus_tag="ACHKCL_001518"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5762517.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLCDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12240..13055)
                     /gene="ysgA"
                     /locus_tag="ACHKCL_001519"
     CDS             complement(12240..13055)
                     /gene="ysgA"
                     /locus_tag="ACHKCL_001519"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5762518.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13317..14078
                     /gene="udp"
                     /locus_tag="ACHKCL_001520"
     CDS             13317..14078
                     /gene="udp"
                     /locus_tag="ACHKCL_001520"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5762519.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14219..15646
                     /gene="rmuC"
                     /locus_tag="ACHKCL_001521"
     CDS             14219..15646
                     /gene="rmuC"
                     /locus_tag="ACHKCL_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5762520.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15741..16496
                     /gene="ubiE"
                     /locus_tag="ACHKCL_001522"
     CDS             15741..16496
                     /gene="ubiE"
                     /locus_tag="ACHKCL_001522"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5762521.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16510..17115
                     /gene="ubiJ"
                     /locus_tag="ACHKCL_001523"
     CDS             16510..17115
                     /gene="ubiJ"
                     /locus_tag="ACHKCL_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5762522.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17112..18752
                     /gene="ubiB"
                     /locus_tag="ACHKCL_001524"
     CDS             17112..18752
                     /gene="ubiB"
                     /locus_tag="ACHKCL_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5762523.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18831..19100
                     /gene="tatA"
                     /locus_tag="ACHKCL_001525"
     CDS             18831..19100
                     /gene="tatA"
                     /locus_tag="ACHKCL_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5762524.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19104..19619
                     /gene="tatB"
                     /locus_tag="ACHKCL_001526"
     CDS             19104..19619
                     /gene="tatB"
                     /locus_tag="ACHKCL_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5762525.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19622..20398
                     /gene="tatC"
                     /locus_tag="ACHKCL_001527"
     CDS             19622..20398
                     /gene="tatC"
                     /locus_tag="ACHKCL_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5762526.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20440..21222
                     /gene="tatD"
                     /locus_tag="ACHKCL_001528"
     CDS             20440..21222
                     /gene="tatD"
                     /locus_tag="ACHKCL_001528"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5762527.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21219..21707)
                     /gene="rfaH"
                     /locus_tag="ACHKCL_001529"
     CDS             complement(21219..21707)
                     /gene="rfaH"
                     /locus_tag="ACHKCL_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5762528.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21874..23367
                     /gene="ubiD"
                     /locus_tag="ACHKCL_001530"
     CDS             21874..23367
                     /gene="ubiD"
                     /locus_tag="ACHKCL_001530"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5762529.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23413..24114
                     /gene="fre"
                     /locus_tag="ACHKCL_001531"
     CDS             23413..24114
                     /gene="fre"
                     /locus_tag="ACHKCL_001531"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5762530.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24306..25469)
                     /gene="fadA"
                     /locus_tag="ACHKCL_001532"
     CDS             complement(24306..25469)
                     /gene="fadA"
                     /locus_tag="ACHKCL_001532"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5762531.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25479..27668)
                     /gene="fadB"
                     /locus_tag="ACHKCL_001533"
     CDS             complement(25479..27668)
                     /gene="fadB"
                     /locus_tag="ACHKCL_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5762532.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27858..29189
                     /gene="pepQ"
                     /locus_tag="ACHKCL_001534"
     CDS             27858..29189
                     /gene="pepQ"
                     /locus_tag="ACHKCL_001534"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5762533.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29189..29803
                     /gene="yigZ"
                     /locus_tag="ACHKCL_001535"
     CDS             29189..29803
                     /gene="yigZ"
                     /locus_tag="ACHKCL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5762534.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29842..31293
                     /gene="trkH"
                     /locus_tag="ACHKCL_001536"
     CDS             29842..31293
                     /gene="trkH"
                     /locus_tag="ACHKCL_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5762535.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31305..31850
                     /gene="hemG"
                     /locus_tag="ACHKCL_001537"
     CDS             31305..31850
                     /gene="hemG"
                     /locus_tag="ACHKCL_001537"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5762536.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7428 a         8125 c         8748 g         7694 t
ORIGIN      
        1 ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg
       61 gagttttggg gggatttcct gtaacaaata ttgttctttt tcttatctta atttttttca
      121 aaaaaagata aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta
      181 actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat
      241 aaaggtgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag
      301 caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgaaggcg
      361 cgtggcagcg gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag
      421 cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc
      481 aaacaggtaa atcgccgcca cgggcagcag ccacatggtt tcaattaaca tgccggtttg
      541 tgcttctacc gcaatcttct tgcgcaccag gccgtagaag gcaaaactaa atgccagccc
      601 cagcgcgata ataggtagcg aaccaaaagt ccacagctgg actaacacgc cacatatcgc
      661 cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag
      721 cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt
      781 gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag
      841 cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat
      901 gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatctcatc
      961 ggcgggcacg tagtaaatca acttgaagta cgctggggct ataccccaaa taaaataagc
     1021 ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt
     1081 ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt
     1141 ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt
     1201 attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcctctgc ctgggcgcag
     1261 ataatcgacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc
     1321 actgatggtt tcgtggcggg ttaaggtact tcctacgcac accagaccgg cggcgacatc
     1381 cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc ccaccatcat
     1441 tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gttccatccc
     1501 caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg
     1561 ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga
     1621 ttttatgatt ctttgttgtt ggtttctact ttaggaaggg ataactaacg gcaatggagt
     1681 taagtatgta aaataccccg cagaaaaaca ttcaccttat caataattcg ttacggagaa
     1741 cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag
     1801 tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta
     1861 ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca
     1921 actacctcat ctacacccaa accagcgatc tgaacaaaga agcgattgcc agttacgact
     1981 gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccgctgt
     2041 ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt
     2101 ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat
     2161 tgttcctcgg ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga
     2221 tggggtataa ccacgactct aacgggcgtt cagacccgac ctcccgcagc tggaaccgcc
     2281 tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg
     2341 tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac taccagctta
     2401 aaatcggcta tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac aactggaaca
     2461 ccggctacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt
     2521 atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc
     2581 gtgtcggtgt gggggttatg ctaaacgatt tgttttgatg aacggttgag tggttggcaa
     2641 atctggaatc cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc
     2701 gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg
     2761 cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc
     2821 aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc
     2881 tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc
     2941 taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc
     3001 aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc
     3061 aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg
     3121 aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag
     3181 ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg
     3241 cgctcggtca gttgcgccag cggttcccga cgctgccgtt tatggcgctg accgccacag
     3301 ccgacgacac cacgcgccag gatatcgtgc gcctgctggg gctgaacgat ccgctgattc
     3361 aaatcagcag ttttgaccgt ccgaatattc gctacatgct gatggagaaa ttcaaaccgc
     3421 tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca
     3481 acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg
     3541 cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc
     3601 gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa
     3661 acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa
     3721 ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat gttgttttac gatccagctg
     3781 atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatatcg
     3841 agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc cgtcgtctgg
     3901 tattgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc
     3961 tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg
     4021 gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga agtgattcgt ggtgctaata
     4081 accagcgtat ccgcgactat ggtcatgaca aactgaaagt ctatggcatg ggccgtgata
     4141 aaagccatga acattgggtg agcgtgatcc gccagctgat tcacctcggc ctggtgacgc
     4201 aaaatattgc ccagcattct gccctacaac tgacagaggc cgcgcgcccg gtgctgcgcg
     4261 gcgaatcctc tttacaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc
     4321 agaaatcgtt cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta
     4381 aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga
     4441 ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg
     4501 ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc gctgattcgt gcgcatgtcg
     4561 atggcgacga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag actccgtaaa
     4621 cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca
     4681 ttgtggcgct tatgagtccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc
     4741 gttcccgtaa agaagcgatg atgggcgtgc tgggtattac ctgcggcgtt atggtttggg
     4801 cagggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc
     4861 tgattatggt tggcggtggc ctgtatctct gctggatggg ttaccagatg ctgcgcggcg
     4921 cgctgaaaaa agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc
     4981 gcagtttcct gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg
     5041 gctcggtgtt ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt
     5101 tcgcgttgat tattgtcgaa acgctggcgt ggtttactgt ggtggccagc ctgtttgccc
     5161 tgccgcaaat gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg
     5221 cgttatttgc cggatttggc attcatttga ttatttcgcg gtaatgccag acgcgtcttc
     5281 agagtaagtc ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc
     5341 cgatgctaac agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt
     5401 catctgcttt ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat
     5461 catcacaata atatcgacca caatagtggt gacaccgagc acgatatact gcatcagttg
     5521 cggctgttgc ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg
     5581 attggtgaga ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc
     5641 cagcgattta agatcaattg cgccagcggc gcgccattgc tgtattccca gccaaatcaa
     5701 gtaagccgcg cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt
     5761 ccccaacccc acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccggcaat
     5821 agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc
     5881 agagcctggc gacaggctta aaatgattga tgtcagtagg taggcaaacc accattctaa
     5941 ggtcatgatg aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt
     6001 tgtgtcaggc acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt
     6061 ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac
     6121 cgctgactga tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc
     6181 cggtgcgctt tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg
     6241 ggcgtattga gagttacgta aaatatgcgg aactggccta cgatttgttc catttggggt
     6301 ttgatgtctt aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc
     6361 cgcatctcgg gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc
     6421 aacaggaggt ccagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg
     6481 gtgcgatctc cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa
     6541 ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca
     6601 actgggccga agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc
     6661 gcgcgttgcc gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact
     6721 tacgcttcta tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac
     6781 gcgaaagtat tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa
     6841 cgcttctctt gcaggctgaa gaggaacgcg tggtggataa ccgcattcat gaccgttttt
     6901 gtgaactccg caccgccgcg ggccatcctg tcgaaggggg acgaccgctg gtaattaaag
     6961 gtgcgtacca tgagatcctt tttgaaaagg acgcaatgcg ctcagttgcg ctccacgcca
     7021 tcgttgattt tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa
     7081 tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat
     7141 acattatccc cttacgccaa agaacccctg aagctgctca ccgcgcgcgg catcaacttt
     7201 gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatt
     7261 aagtcttaca tgattacctc caatggtgcg cgcgttcacg atctggatgg taatctgatt
     7321 tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat
     7381 ccggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg
     7441 gaagagatgc gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta
     7501 ctggagccgg aaggcgtcag caaagtgttc ttcacctgcg attcccatga acaactgctg
     7561 ccgctggagc aggcgattaa cgctcgttgg ggcgatcgcg tcaacgtcag tttctctacc
     7621 ttaacctgtc tggaagtgat ggcgggcggc gtttcaaaag gccatgcgct ggaagcggtg
     7681 gcgaagaaac tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgat
     7741 gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg
     7801 aaagaccttc atcccgagct ggaagtgatt ggtactaatg ccgacgacgc ggtgccgcat
     7861 tatctgcgta aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata
     7921 cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc
     7981 accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc
     8041 gatagctatt ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt
     8101 ctgcgtaccc gtggcaatag cctgaaaaca gtcggcctgt atatgctggt gggcgcgatg
     8161 cagctcggcg tgatgtatat gctgagtttc cgcgcttatc tctacctgac ggtttccgaa
     8221 ctgctgctgt tcaccgtact gacgccgctc tacatcacgc tgatttatga catcatgagt
     8281 aagcgccgtc tgcgctgggg atatgctttt agtgccttgt tggcggtgat tggtgccggg
     8341 attattcgct atgatcaggt caccgaccat ttctggactg gcttgttgct ggtgcaactc
     8401 tccaatatca cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg
     8461 atgccgcagc ataacgcctt tgcgtggttc tatcttggcg cgttcctggt ggcggtaatt
     8521 gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt
     8581 ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgca
     8641 acccaagtag acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg
     8701 ctggttaacc tggctatctg gcaccaacag ccgcactggc caacgtttat tacaggcgcg
     8761 ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa
     8821 acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat aaacgcttcc gtaactggct
     8881 gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg
     8941 ttttggtcac caccagaccc tggcgctcaa aactctctac tacccaatgt ggtagcgcgg
     9001 caatacccat ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca
     9061 gcgacgggct aacgcctgcg ggctggagaa aatgccgcca gacatccagt cggctacgct
     9121 gcaccggata aatcaacagc gtttcgctgg cgagatcttc cggcgtgatt cgcgttttcg
     9181 ccgccagtgg atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat
     9241 aatgcaggcc actacgcggc agaatatcgg aggtcatcac cagatccagc tcgccctgct
     9301 gcaacgcagg ctgcgggtca aacgttacgc ccgatttaaa atccatctcc acctgcggcc
     9361 agttcttatg gaaattttct aatgcgggcg tcagccactg aatacagcta tggcactcaa
     9421 tggcaatgcg caggcgcgtc tgttgcggtt cattgcaggc ttgcagcgcc tggctaattt
     9481 gcggcagcac ctggtttgcc agttgcaaca ggatttcccc ctgtggagta aagcgtagcg
     9541 gctggctctt acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt
     9601 gagacagggc ggattgcgtt tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt
     9661 tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg
     9721 gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat
     9781 ctggacggct aaaatccttc gtcttttaaa tttatggtgc gttggctgcg tttctccacc
     9841 ccggtcactt acttcagtaa gctcccgggg atgaataaac ttgccgcctt ccctaaattc
     9901 aaaatccata ggatttacat ataattagag gaagaaaaat gacaatattg aatcacaccc
     9961 tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagag agttattggg
    10021 cggggaactc cacgcgtgaa gaactgctgg cggtagggcg tgaattgcgt gctcgtcact
    10081 gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt gcctggtacg
    10141 atcatgtact gaccaccagt ctgctgctgg gtaacgttcc ggcgcgtcat cagaacaaag
    10201 atggttcggt agatatcgac accctgttcc gtattggtcg tggacgtgcg ccgactggcg
    10261 aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac tacatggtgc
    10321 cggagttcgt taaaggccaa cagttcaaac tgacctggac gcagctgctg gacgaagtgg
    10381 acgaggcgct ggcgctgggc cacaaggtga aacctgtgct gctggggccg attacctggc
    10441 tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct gagcctgctg aacgacattc
    10501 tgccggttta tcagcaagtg ctggcagaac tggcgaaacg tggcatcgag tgggtacaga
    10561 ttgatgaacc cgcgctggta ctggaactac cacaggcgtg gctggacgca tacaaacccg
    10621 cttacgacgc gctccaggga caggtgaaac tgctgctgac cacctatttt gaaggcgtaa
    10681 cgccaaatct cgacacgatt actgcgctgc ctgttcaggg gctgcatgtg gaccttgtac
    10741 atggtaaaga tgacgttgtt gaactgcaca agcgcctgcc ttctgactgg ctgctgtctg
    10801 cgggtctgat caatggtcgt aacgtctggc gcgccgatct tactgagaaa tatgtgcaaa
    10861 ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca
    10921 gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct
    10981 tcgccctaca aaaatgccat gaactggcac tgctgtgcga tgcgctgaac agtggtgaca
    11041 cggcagctct ggcagagtgg agcgccccaa ttcaggcgcg tcgtcactct actcgtgtac
    11101 ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga
    11161 atgtctatga agtgcgtgct gaagcccagc gtgcgcgttt taaactgcca gcgtggccga
    11221 ccaccacgat tggttccttc ccgcaaacca cggaaattcg taccctgcgt ctggatttca
    11281 aaaagggcaa tctcgacgcc aataactacc gcacgggcat tgcggaacat atcaggcagg
    11341 ccattgttga acaggaacgt ttgggactgg atgtgctggt acatggcgag gccgagcgta
    11401 atgacatggt ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg caaaacggtt
    11461 gggtacagag ctacggttcc cgctgcgtga agccaccgat tgttattggt gacgttagtc
    11521 gcccggcacc gattaccgtg gagtgggcga agtatgcgca atctatgact gataaaccgg
    11581 tgaaagggat gctgactggc ccggtgacca ttctctgctg gtcgttcccg cgtgaagatg
    11641 tcagccgtga aaccatcgcc aaacaaattg cgctggcgct gcgtgatgaa gtggcggacc
    11701 tggaagccgc aggaattggc atcatccaga ttgatgaacc ggcgctgcgc gaaggtttac
    11761 cgctgcgtcg tagcgactgg gatgcatatc tccagtgggg cgtggaggcc ttccgtatca
    11821 acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttat tgcgagttca
    11881 acgacatcat ggattcgatt gcggcactgg acgcagacgt catcaccatc gaaacctcgc
    11941 gttctgacat ggagttgctg gagtcgttcg aagagtttga ttatccgaat gaaatcggtc
    12001 caggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt gaagccttgc
    12061 tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg ggtcaacccg gactgtggcc
    12121 tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg caggcggcgc
    12181 agaacttgcg tcgggggtaa aatccaaacc gggtggtaat accacccggt cttttctcat
    12241 tacagcgact tcttcccacc atactgctta aaccattcca gcatacgctg ccagccatct
    12301 tctgcagatg cggcatgata gctcgggcga taatcagcgt tgaatgcatg cccggcgtcc
    12361 gggtacacga taatctctgc tttcgcatta gcagcccgca gcgcctggcg catggtttca
    12421 acgctctcct gcggaatact gttatccaga ccgccatata agccgagaac cggcgcgtta
    12481 agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt
    12541 ttgccgtacc acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaagtg
    12601 atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg
    12661 gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct
    12721 accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag
    12781 taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg
    12841 atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag tgggccatcg
    12901 ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg
    12961 aagcccgcca caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca
    13021 gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa
    13081 tgttgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat
    13141 cttttatgca gtcctgcaga atgaagagtg atttatgtga tttgcatcac ctttggtggg
    13201 taaatttatg caacgcattt gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct
    13261 tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt
    13321 ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg
    13381 ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg
    13441 ttaagctggc atctcaccgc gaattcacta cctggcgtgc agagctggat ggtaaacctg
    13501 ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg
    13561 cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata
    13621 ttaatgtggg tgatgtcctg gttaccacgg cgtctgtccg tctggatggc gcgagcctgc
    13681 acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg
    13741 aagctgcgaa atccattggc gcgacaactc acgttggcgt gacagcttct tctgatacct
    13801 tctacccagg tcaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag
    13861 gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc
    13921 tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt gttatcgtta
    13981 accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaactgaa agccaagcgg
    14041 tgaaaatcgt ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc
    14101 cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg
    14161 gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt
    14221 ggatttctca atcatggttt acgcagttat tgcgttggtg ggtgtggcaa ttggctggct
    14281 gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat
    14341 ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact ggcgtgcaga
    14401 gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc
    14461 cgatctgcgt gaagtaacca cgcggatgga agccgcacag caacatgctg acgataaaat
    14521 tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg
    14581 tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct
    14641 gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg acagcttcgg
    14701 taaagaagca caagaacgcc ataccctgac ccacgaaatt cgcaatctcc agcaactcaa
    14761 cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac
    14821 ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg gtctgcgtga
    14881 agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc
    14941 ggatgtcatc gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct
    15001 ggtcgcctat gaacgctatt ttaacgccga agacgactac acccgcgaaa gcgcgctaca
    15061 ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca
    15121 gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg aacccgcttt
    15181 tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat
    15241 gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc tgtggcgtta
    15301 tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa
    15361 gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca aagcgcagga
    15421 taactatcgg caggcaatga aaaaactctc ttcagggcgc ggaaatgtgc tggcgcaggc
    15481 agaagcgttt cgcggtttag gagtagaaat taaacgcgag attaatccgg atttggctga
    15541 acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc agccgaatga
    15601 tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca ttgggagtag
    15661 ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt
    15721 tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc
    15781 agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat
    15841 caaaatacga tgtcatgaat gatttgatgt catttggtat tcatcgtttg tggaagcgat
    15901 tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca
    15961 ccggcgacct gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa gtggtccttg
    16021 ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat atcggtgtga
    16081 ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct
    16141 ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc
    16201 gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc
    16261 caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta
    16321 ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta
    16381 tgcatcccga tcaggatacc ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg
    16441 actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag ttctgacagg
    16501 agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc
    16561 ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg caaagtgctg
    16621 cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtt
    16681 gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgccta cgccagtgtg
    16741 ttgccgaaac ttcgcgatcg ccagcagctt gccgccctga ttcgcagtgg tgagctggaa
    16801 gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcaggtct ggcagagttc
    16861 gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa
    16921 gccatgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg
    16981 gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt
    17041 gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgatcaaacg gctggaaaaa
    17101 ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt
    17161 taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat
    17221 ggcgatactc attattctgg atgccaaatc ggcataaaga caaactttta ggtgagcgac
    17281 tacgactggc cctgcaagaa ctggggccgg tatggatcaa gttcgggcaa atgttatcaa
    17341 cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg caggacaaag
    17401 ttgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc
    17461 cggtagaagc gtggtttgac gattttgaaa tcaagccact ggcttctgct tctatcgccc
    17521 aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc
    17581 cggatatttt gccggtcatt aaagcagacc tgaaacttat ctaccgtctg gctcgctggg
    17641 tgccgcgttt gctgccggat ggtcgccgtc tacgcccaac cgaagtggtg cgcgagtacg
    17701 aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc
    17761 ggcgaaattt tgaagacagc ccgatgctct acatcccgga ggtttaccct gactattgta
    17821 gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat gttgcggcgc
    17881 tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca
    17941 ctcaggtctt tcgcgacagc tttttccatg ccgatatgca ccctggcaac atcttcgtaa
    18001 gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc
    18061 taaacaaaga agataaacgc tatctggcag aaaactttat tgccttcttt aatcgcgact
    18121 atcgcaaagt ggcagagcta cacgtcgatt ctggctgggt gccaccagat accaacgttg
    18181 aagagttcga atttgctatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg
    18241 aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg
    18301 aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac
    18361 gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga
    18421 ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct
    18481 gggtcgaaaa aatgccagaa ctgcctgaat tggtttacga cagtttgcgc cagggcaagt
    18541 atttacagca cagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg
    18601 gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt
    18661 tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt ggtctgatcg
    18721 cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt
    18781 aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta
    18841 tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa
    18901 agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga
    18961 gcgatgatga accaaagcag gataaaacca gtcaggatgc tgattttact gcgaaaacta
    19021 tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc
    19081 acgataaaga gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg ctattggtgt
    19141 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
    19201 cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
    19261 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
    19321 acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga
    19381 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
    19441 acccggtggt gaaagataat gaagctgcgc atgagggtgt aacgcctgcc gctgcacaaa
    19501 cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg
    19561 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa
    19621 catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc gtaagcgtct
    19681 gctgaactgc attatcgcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga
    19741 catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat
    19801 cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct
    19861 gattctgtca gcgccggtga ttctctatca ggtgtgggca tttatcgccc cagcgctgta
    19921 taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tattttatat
    19981 cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac
    20041 cgcgccggaa ggggtgcagg tatccaccga catcgccagc tatttaagct tcgttatggc
    20101 gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc tgctgtgctg
    20161 gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc
    20221 attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc tgttggcgat
    20281 cccgatgtac tgtctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg
    20341 gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat
    20401 tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat
    20461 ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg
    20521 ggagttaatg ggctactcat caccggcact aacctgcgtg aaagccagca ggcgcaaaag
    20581 ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc
    20641 cagtggcaag ctgcgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg
    20701 gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa
    20761 cgcgcttttg ttgcccagct acgcattgcc gcagatttaa acatgccggt atttatgcac
    20821 tgtcgcgatg cccacgagcg gtttatgaca ttactggagc cgtggctgga taaactgcct
    20881 ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcat
    20941 ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcggact ggagctgcgg
    21001 gaacttttgc cgttgattcc ggcggaaaaa ttactgatcg aaactgatgc gccgtatctg
    21061 ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc
    21121 catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc
    21181 acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg
    21241 ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc
    21301 tcaccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc ggtaataatc
    21361 accttatcac ccggataagg ggttgccgga tcgacaatgt ctttcggttt atataccgat
    21421 agctgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg cacgaagtgg
    21481 ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc aaattccaca
    21541 aacaggtagt tggggaacaa tggctcactg actgcagtac gtttgccacg cacgattttt
    21601 tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca
    21661 cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc
    21721 cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt
    21781 ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa
    21841 cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata taacgattta
    21901 cgcgacttct tgacgttgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg
    21961 gacccgcatc tggaaatcac tgaaattgct gaccgcacgc tgcgtgctgg tgggcctgcg
    22021 ctgttgtttg aaaaccctaa agggtactca atgccggtgc tgtgcaactt gttcggtacg
    22081 ccaaagcgcg tagcgatggg tatgggccag gaagatgttt cagcactgcg tgaagtcggt
    22141 aaattattag catttctgaa agaaccagag ccgccaaaag gttttcgcga tctgtttgat
    22201 aagctgccgc agtttaagca ggtgttaaac atgccgacaa agcgactgcg cggtgcaccc
    22261 tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca accgtattcc cattatgacc
    22321 tgttggccgg aagatgccgc gccgctgatt acatgggggc taaccgttac acgtggccct
    22381 cataaagagc gacagaatct gggcatttat cgccagcaac tgattggtaa aaacaagctg
    22441 attatgcgtt ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg
    22501 catccaggtg aacgtttccc gatctctgtg gcgttgggcg ctgatccggc aaccattctc
    22561 ggtgcagtca caccagtacc agatactttg tcggaatacg cctttgccgg attgctacgt
    22621 ggcaccaaaa ccgaagtagt gaagtgtatt tccaatgatc tcgaagtgcc cgccagtgcg
    22681 gagattgtgc tggaagggta tatcgaacaa ggcgaaatgg cgccagaagg accgtatggt
    22741 gaccacactg gttactataa cgaagtcgat agtttcccgg tatttaccgt gacgcatatt
    22801 acccagcgtg aagatgcgat ttaccattcc acctataccg ggcgtccgcc agatgaaccc
    22861 gcggtactgg gagtggcgtt gaacgaagta tttgttccca ttctgcaaaa gcagttcccg
    22921 gaaattgtcg atttttacct gccgccggaa ggctgctctt atcgcctggc ggtagtgaca
    22981 atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta
    23041 cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgttaa cgcacgcgac
    23101 tggaacgatg tgatttgggc gattaccacc cgtatggacc cagcgcggga tactgttctg
    23161 gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca
    23221 aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt
    23281 cccatcaaaa aagatccaga tgttgtcgca catattgacg ccatctggga tgaactggct
    23341 atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga
    23401 cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat
    23461 accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc tggtcagtat
    23521 ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcctc gacgccggat
    23581 gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca
    23641 gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg
    23701 tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct
    23761 tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt
    23821 tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg
    23881 ttgaagcatc ctggtctgca agtggtgccg gtggttgaac aaccggaagc gggctggcgt
    23941 gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc acggtacgct ggcagagcat
    24001 gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct gttttgcagt
    24061 gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata
    24121 aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcattt
    24181 agatctatgt cgcatccggc attcggcaca cgatgcctga tgcgacgctg gcgcgtctta
    24241 tcaggcctac attggtgccg gatcggtaga ccggataagg cgttcacgcc gcatccggca
    24301 agtggttaaa cccgctcaaa caccgtcgca ataccctgac ccagaccgat acacatcgtc
    24361 gccagaccaa actgaacgtc tttgtgttcc atcagattca gcagcgtggt gctgatacgc
    24421 gcaccggaac aacccagcgg atgacccagc gcgatcgcgc caccgttgag gttgatcttc
    24481 tcgtcaatct gctcaattag tcccagatct ttaatacatg gcaggatctg cgcggcaaag
    24541 gcttcattca tttcaaacac gccgatatca ctgacagaaa gccccgcttt tttcagcgcc
    24601 agcttcgagg ccggaaccgg gccgtaaccc atgatcgatg ggtcacaacc aacgaccgcc
    24661 atcgaacgca cacgagcgcg cggcttaaga cctaattcat gggcgcggct ttcactcatc
    24721 accagcatgg cagctgcgcc atcggaaagt gcagaagatg tgcccgccgt taccgtaccg
    24781 tttactggat caaacgccgg acgcagcgtg gcgagggctt ccacggtggt ttccgggcga
    24841 atcacttcgt cgtaattaaa ctgcttcagg acgccgtcgg catcgtgacc accagttggg
    24901 atgatttcat ttttaaatgc gcccgactgc gtggcggccc aggcgcgggc gtgtgaccgc
    24961 gcggcaaagg catcctgcat ttcacggctg ataccgtgca tacgcgccag catttctgcc
    25021 gttaagccca tcatgcccgc cgctttggcg acattgcggc tcaggccagg gtgaaaatcg
    25081 acgccgtgac tcatcggcac atggcccata tgctccacgc cgccaaccag acatgcctgc
    25141 gcatcgccgg tcatgatcat tcgtgctgcg tcatgcagtg cctgcatgga tgaaccacac
    25201 aagcgattga cagtaaccgc cgggacagag tgcggcactt ctgccagcag cgccgcgtta
    25261 cgggcgatat taaaaccctg ctccagcgtc tgctgcacac acccccagta aatatcgtcg
    25321 agggccgccg cttccagcgc cgggttacgc gccagcaggc tacgcattaa atgagcggag
    25381 agatcttctg cacgcacgtt acgaaaagca ccgcccttcg aacggcccat cggggtgcga
    25441 attgcatcga caatgacaac ctgttccatt gtgactcctt aagccgtttt caggtcgcca
    25501 accggacggg ctggctcaac cgggggatag tacggttcgt tatgacgcgc tttattacgc
    25561 agaccttccg gcacttcgta cagcgggccg aggtgctgat attgctgtgc catatcgagg
    25621 tattttgcgc tgccgagggt gtccagccag cggaacgcgc cgccgtggaa cggagggaag
    25681 cccaggccgt agaccagcgc catatccgct tctgccggag tggcgataat gccttcttcc
    25741 agacagcgca ccacttcgtt gaccatcggg atcatcatgc gggcgataat ctcttcttcg
    25801 ctgaaatcgc gcttcggctg gctcacttct gccagcaggt cgtcaacagc ggcgtcttct
    25861 tctttcttcg gcttaccttt gctgtcttct ttataatgcc agaaaccgag gccgttcttc
    25921 tgaccaaagc ggttggcatc aaacagcgcg tcgatggcat cgcgataatc tttctgcatc
    25981 cgctgtggga agcccgctgc catgactgcc tgggcgtgat gcgcggtgtc aatgcccaca
    26041 acgtccagca gatatgccgg acccataggc cagccaaact gtttttccat cactttgtcg
    26101 atcttgcgga aatccgcgcc gtcgcgcagc aactggctga aaccggcgaa atacgggaac
    26161 agcacgcggt tgacgaagaa gccggggcaa tcgttaacca caatcggcgt tttacccatc
    26221 ttgctcgccc aggcgacgac tttcgcaata gtttcgtcgg agcttttctc gccgcgaata
    26281 atttccacca acggcattcg gtggaccggg ttaaagaagt gcatcccgca gaagttttcc
    26341 ggacgttcca gcgcgttggc cagttcgctg ataggaatgg ttgaagtgtt agacgccagc
    26401 acggtatccg ggcgtacttt ctgctcggtt tctgccagca cggctttttt cactttcggg
    26461 ttttcaacaa tcgcttctac cacaacatcc acgcgctcaa atccggcgta gtcgagcgtt
    26521 gggtggatcg tggagatcac gccagccagt ttcagaccat cgatcttgcc gcgctcaagc
    26581 tgcttgttca gtagtttcgc ggcttcagtc atgccgaggg ctaacgactt gtcgttaata
    26641 tctttcataa tcaccggcac gcctttccac gcagactggt aagcgatgcc gccacccata
    26701 atgcctgcgc ccagcacggc ggcctgtttc ggggtttcaa cgtctttggt gagtttcttc
    26761 gctttgcctt ttacatattg atcgttaagg aaaatgccga ccagtgcgcg agcttcgttg
    26821 gtatgcgcca gcgggacaaa gcttttgttt tctaggttta aggcttcttc acgaccaaaa
    26881 cgagccgcag cttcaatggt ttttactgcg gtgatggggg ccgggtaatg ttttcctgcc
    26941 gtttgtgcga ccatcccttt agcgatggtg aagctcatgg tggcttcaat cttgctcagt
    27001 tttagcggtt ccagcttcgg ctgacgtttc gctttccagt cgaggtcgcc gttaatggcc
    27061 tggcgtaaaa tcgccatcgc cccttcaatc agtttttctg ctttgactac gccatccacc
    27121 agaccgattt tcagcgcctg atccgcgccg acatctttac cggcggcaat gatttccagc
    27181 gcactgtcag cgcccagcat acgtggcata cgtacagaac cgccaaagcc cggcatgatg
    27241 cccagtttgg tttccggcag accgatgcgc agatccggcg tcgccagacg gtaatcggtc
    27301 gccagcacgc attcgcagcc accgcccagc gcatagccat tgacggcagc aatggtcggc
    27361 accggcagat cttccaggcg attaaacacg ctattggcaa agtgcagcca ctgacttaac
    27421 tgttcttcag gaacgaggaa cagggacaaa aattcggtga tatcagcacc gacgataaag
    27481 gctgctttgt tcgaacgcag cagcagccct tttagatctg attgctgttc cagcacgccg
    27541 atggcctcgc cgaggctggc gacggtcgca gtgtcgagtt tattaactga acctggggca
    27601 tcaaatacca gttcggcaat gccatcttcc agccagtcaa ggtacagggt gtcgcctttg
    27661 taaagcatgt cagtctcctg aatccgcaag gtgatctggt cgtaccagat gcatcgaagt
    27721 gtgtattttg tgttaaaaat atgcaaataa aagattaaag aaatgccgat ctgatcacgc
    27781 tcggcagaaa tcacgctctg gatgaacgat gtgctaagat gcggagactt aaggtcaaaa
    27841 aaacagaagg gtaaaaaatg gaatcactgg cctcgctcta taaaaatcat atagctacat
    27901 tgcaggaacg aactcgcgat gcgctgacgc gcttcaagct ggatgcgtta cttattcact
    27961 ccggcgaact gttcaacgtt tttctcgacg atcatcccta tccgtttaaa gtgaacccgc
    28021 aattcaaagc gtgggtgccg gtaactcagg tgccaaactg ctggctgctg gtggatggcg
    28081 tgaataagcc gaaactgtgg ttctatctgc cggttgatta ctggcacaac gtcgaaccgc
    28141 tgccgacctc cttctggact gaagatgtag aagtgatcgc gctgccgaaa gccgatggca
    28201 ttggtagtct gttgcctgct gcgcgcggca atatcggtta tatcggtccg gtgccggaac
    28261 gtgcgctgca actgggtatt gaggccagca atatcaaccc gaaaggggtt atcgactacc
    28321 tgcattacta ccgctccttc aaaaccgagt acgagctggc ctgtatgcgt gaagcgcaga
    28381 aaatggcggt caacggtcat cgcgcggcag aagaagcgtt ccgttctggc atgagcgagt
    28441 tcgatatcaa tattgcctat ctgactgcga ccggtcatcg tgataccgac gtaccttaca
    28501 gcaacattgt ggcactcaac gaacacgctg cggtgctgca ttacaccaaa ctggatcatc
    28561 aggcgtcgga agagatgcgc agcttcctgc tggatgccgg ggccgaatat aacggctatg
    28621 ccgctgacct gacccgtacc tggtcggcaa aaagtgacaa cgattacgca cagctggtga
    28681 aagacgtaaa tgatgaacaa ctggcgctga tcgcgaccat gaaagctggc gttagctatg
    28741 tggattacca catccagttc catcagcgca tcgccaaatt gctgcgtaaa catcaaatca
    28801 tcaccgatat gagtgaagag gcgatggtcg aaaacgatct taccgggccg tttatgccgc
    28861 atggtatcgg ccatccgctg ggcctgcagg tgcatgacgt cgccggtttt atgcaggatg
    28921 atagcggtac gcacctcgcg gcaccggcaa aatatccgta cctgcgctgc acccgtattc
    28981 tccagccggg catggtgtta accatcgaac cgggtatcta cttcattgaa tcgctgctgg
    29041 caccgtggcg tgaagggcag ttcagcaagc acttcaactg gcagaaaatt gaagcactga
    29101 aaccgttcgg cggcattcgt atcgaagaca acgtggtgat ccacgaaaat aacgtggaaa
    29161 acatgacccg ggatctgaaa ctggcgtgat ggaaagctgg ttaattcctg cggcaccggt
    29221 cacggtcgtt gaagagatca aaaaaagccg cttcattacg ctgttggcgc ataccgatgg
    29281 cgttgaggcg gcgaaagcgt ttgttgaatc ggtgcgggca gaacaccccg atgcccgcca
    29341 ccattgcgtg gcgtgggtgg cgggtgcgcc ggatgattct caacagctgg gtttctctga
    29401 cgacggggag ccggcgggaa cggcaggtaa accgatgctc gcccagctaa tgggcagcgg
    29461 cgttggggaa attaccgctg tggtggtgcg ctactacggc ggcatattgc ttggcaccgg
    29521 tgggttagtg aaagcgtatg gcggcggcgt gaatcaggcg ctgcgccagc taacgaccca
    29581 acgcaagacg ccattaaccg aatatacttt gcaatgtgaa tatagtcagt taaccggcat
    29641 tgaagcgttg ctggggcagt gtgacggcaa aattatcaac agtgattatc aggcattcgt
    29701 tctgctgcgg gtggcgcttc cggcggcgaa agtggctgaa ttttccgcga agctggcgga
    29761 ttttagccgt ggttcattgc aattgttagc gattgaagaa taatccccac ttcgttttgc
    29821 agaactaagg aagcggcaga gatgcatttt cgcgccatta cccgaatcgt tggactactg
    29881 gtcatcttat tttcagggac catgattatc cctgggctgg tagcactcat ctaccgggat
    29941 ggagcgggcc gcgcttttac ccagaccttt tttgtcgccc tcgccattgg ctctatgctg
    30001 tggtggccga accgcaaaga gaagggcgaa ctgaaatccc gtgaggggtt tctgatagtg
    30061 gtgctgttct ggaccgtgct gggtagtgtc ggtgcgctcc cttttatctt ctcggaaagc
    30121 ccgaacctca cgattaccga tgcgtttttt gaatctttct ctggcctgac caccaccggg
    30181 gccactacgc tggtggggct ggattcgctc cctcatgcca tcctctttta tcgccagatg
    30241 ctgcaatggt ttggcgggat ggggatcatc gtgttagcgg ttgcgatact gcctatcctc
    30301 ggcgtgggtg ggatgcagct ctatcgcgca gaaatgcccg gcccgctgaa agataacaaa
    30361 atgcgcccgc gaattgcgga aacggcgaaa accctgtggt tgatttatgt cttgctgacc
    30421 gtcgcctgtg cgctggcgtt gtggtttgcc ggaatggatg cctttgatgc catcggccat
    30481 agctttgcga ctatcgctat tggcggcttc tcgacacatg atgccagtat cggttatttc
    30541 gatagcccga ctattaacac tatcattgct atcttcctgc tgatctccgg ctgtaactac
    30601 ggtctgcact tttcactgtt aagtgggcgt agtctgaagg tttattggcg cgatccggaa
    30661 tttcgcatgt ttatcggcgt acagtttacg ctggtggtta tttgtaccct cgtactgtgg
    30721 tttcataatg tctacagttc ggcgctgatg acaattaacc aggcgttttt ccaggtggtg
    30781 tcgatggcga caaccgccgg gtttacgact gacagcattg cccgctggcc gctctttttg
    30841 ccggtactgc ttttatgttc agcttttatc ggcggttgtg ccgggtcaac gggcggtggc
    30901 ctgaaagtga tccgcatcct gctgctgttt aagcagggga accgtgaact gaaacgactg
    30961 gtgcatccga acgccgtcta tagcattaag ctggggaatc gcgcactgcc ggaacgtatc
    31021 ctcgaagccg tttggggatt tttctccgcc tatgcattgg tgtttattgt cagtatgctg
    31081 gcgattatcg ccacgggcgt ggatgacttt tctgcctttg cctcggttgt tgcgacattg
    31141 aataacctgg ggccagggct tggcgtggtt gctgataact ttaccagtat gaacccggtg
    31201 gctaaatgga tcctgattgc caacatgctg tttggtcgtc tcgaggtctt tacattgctg
    31261 gtgctcttta ccccgacttt ctggcgtgaa tgatggagta atacgtgaaa acattaattc
    31321 ttttctcaac aagggacgga caaacgcgcg agattgcctc ctacttggct tcggaactga
    31381 aagaactggg gatccaggcg gatgtcgcca atgtgcaccg cattgaagaa ccacagtggg
    31441 aaaactacga ccgtgtggtc attggtgctt ctattcgcta tggtcactac cactctgcgt
    31501 tccaggaatt tgtcaaaaag catgcgacgc ggctgaattc gatgccgagc gcgttttact
    31561 ccgtaaacct ggtggcgcgt aaaccggaga agcgcactcc acagaccaac agctacgcgc
    31621 ggaagtttct gatgaactcg caatggcgtc ccgatcgctg cgcggtcatt gccggggcgc
    31681 tgcgttaccc acgttatcgc tggtacgacc gttttatgat caagctgatt atgaagatgt
    31741 caggcggtga aacggatacg cgcaaagaag ttgtctatac cgattgggag caggtggcga
    31801 atttcgcccg agaaatcgcc catttaaccg acaaaccgac gctgaaataa gcataaagaa
    31861 taaaaaatgc gcggtcagaa aattatttta aatttcccct tgtcaggccg gaataactcc
    31921 ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt cagcgggatt
    31981 ctcctgagaa ttccg
//
LOCUS       DAZFKY010000038        31937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFKY010000038 DAZFKY010000000
VERSION     DAZFKY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..723)
                     /locus_tag="ACHKCL_001538"
     CDS             complement(<1..723)
                     /locus_tag="ACHKCL_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5762537.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNS"
     gene            complement(901..1812)
                     /gene="gsiD"
                     /locus_tag="ACHKCL_001539"
     CDS             complement(901..1812)
                     /gene="gsiD"
                     /locus_tag="ACHKCL_001539"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5762538.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1815..2735)
                     /gene="gsiC"
                     /locus_tag="ACHKCL_001540"
     CDS             complement(1815..2735)
                     /gene="gsiC"
                     /locus_tag="ACHKCL_001540"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5762539.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2753..4291)
                     /gene="gsiB"
                     /locus_tag="ACHKCL_001541"
     CDS             complement(2753..4291)
                     /gene="gsiB"
                     /locus_tag="ACHKCL_001541"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5762540.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4311..6182)
                     /gene="gsiA"
                     /locus_tag="ACHKCL_001542"
     CDS             complement(4311..6182)
                     /gene="gsiA"
                     /locus_tag="ACHKCL_001542"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5762541.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6169..7134)
                     /gene="iaaA"
                     /locus_tag="ACHKCL_001543"
                     /pseudo
     CDS             complement(6169..7134)
                     /gene="iaaA"
                     /locus_tag="ACHKCL_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            7338..8573
                     /gene="moeA"
                     /locus_tag="ACHKCL_001544"
     CDS             7338..8573
                     /gene="moeA"
                     /locus_tag="ACHKCL_001544"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5762542.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8573..9322
                     /gene="moeB"
                     /locus_tag="ACHKCL_001545"
     CDS             8573..9322
                     /gene="moeB"
                     /locus_tag="ACHKCL_001545"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5762543.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9501..10163)
                     /gene="fsa"
                     /locus_tag="ACHKCL_001546"
     CDS             complement(9501..10163)
                     /gene="fsa"
                     /locus_tag="ACHKCL_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5762544.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            10294..11193
                     /gene="ybiY"
                     /locus_tag="ACHKCL_001547"
     CDS             10294..11193
                     /gene="ybiY"
                     /locus_tag="ACHKCL_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5762545.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11199..13630
                     /gene="ybiW"
                     /locus_tag="ACHKCL_001548"
                     /pseudo
     CDS             11199..13630
                     /gene="ybiW"
                     /locus_tag="ACHKCL_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            13776..14591
                     /gene="ybiV"
                     /locus_tag="ACHKCL_001549"
     CDS             13776..14591
                     /gene="ybiV"
                     /locus_tag="ACHKCL_001549"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5762546.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14743..16008
                     /gene="ybiU"
                     /locus_tag="ACHKCL_001550"
     CDS             14743..16008
                     /gene="ybiU"
                     /locus_tag="ACHKCL_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5762547.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16176..17768)
                     /gene="ybiT"
                     /locus_tag="ACHKCL_001551"
     CDS             complement(16176..17768)
                     /gene="ybiT"
                     /locus_tag="ACHKCL_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5762548.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17987..18907
                     /gene="ldtB"
                     /locus_tag="ACHKCL_001552"
     CDS             17987..18907
                     /gene="ldtB"
                     /locus_tag="ACHKCL_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5762549.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVLVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18965..20083)
                     /gene="ybiR"
                     /locus_tag="ACHKCL_001553"
     CDS             complement(18965..20083)
                     /gene="ybiR"
                     /locus_tag="ACHKCL_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5762550.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20080..20547)
                     /gene="mntR"
                     /locus_tag="ACHKCL_001554"
     CDS             complement(20080..20547)
                     /gene="mntR"
                     /locus_tag="ACHKCL_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5762551.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20733..20861
                     /gene="mntS"
                     /locus_tag="ACHKCL_001555"
     CDS             20733..20861
                     /gene="mntS"
                     /locus_tag="ACHKCL_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5762552.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21133..22716
                     /gene="opgE"
                     /locus_tag="ACHKCL_001556"
     CDS             21133..22716
                     /gene="opgE"
                     /locus_tag="ACHKCL_001556"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5762553.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22765..23280)
                     /gene="ompX"
                     /locus_tag="ACHKCL_001557"
     CDS             complement(22765..23280)
                     /gene="ompX"
                     /locus_tag="ACHKCL_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5762554.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23333..23398)
                     /locus_tag="ACHKCL_001558"
     CDS             complement(23333..23398)
                     /locus_tag="ACHKCL_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762555.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23633..24520
                     /gene="rhtA"
                     /locus_tag="ACHKCL_001559"
     CDS             23633..24520
                     /gene="rhtA"
                     /locus_tag="ACHKCL_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5762556.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24819..25322
                     /gene="dps"
                     /locus_tag="ACHKCL_001560"
                     /gene_synonym="pexB"
     CDS             24819..25322
                     /gene="dps"
                     /locus_tag="ACHKCL_001560"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5762557.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25726..26472
                     /gene="glnH"
                     /locus_tag="ACHKCL_001561"
     CDS             25726..26472
                     /gene="glnH"
                     /locus_tag="ACHKCL_001561"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5762558.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26610..27269
                     /gene="glnP"
                     /locus_tag="ACHKCL_001562"
     CDS             26610..27269
                     /gene="glnP"
                     /locus_tag="ACHKCL_001562"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5762559.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27266..27988
                     /gene="glnQ"
                     /locus_tag="ACHKCL_001563"
     CDS             27266..27988
                     /gene="glnQ"
                     /locus_tag="ACHKCL_001563"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5762560.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            28105..30330
                     /gene="ybiO"
                     /locus_tag="ACHKCL_001564"
     CDS             28105..30330
                     /gene="ybiO"
                     /locus_tag="ACHKCL_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO5762561.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(30327..31253)
                     /gene="rlmF"
                     /locus_tag="ACHKCL_001565"
     CDS             complement(30327..31253)
                     /gene="rlmF"
                     /locus_tag="ACHKCL_001565"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5762562.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31529..31789
                     /gene="mcbA"
                     /locus_tag="ACHKCL_001566"
     CDS             31529..31789
                     /gene="mcbA"
                     /locus_tag="ACHKCL_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5762563.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         7802 a         8558 c         7922 g         7655 t
ORIGIN      
        1 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatggcc
       61 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
      121 tcgccagttt aaatcgacat gaattttatc caccgctaac aaatcgtgat ctttgccgtt
      181 gatgcgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtaggccgg
      241 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
      301 atcggcaaaa ataacctgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
      361 ataaacaacg attttatcgt tcgccctccg cgaaaagaga taatatttat tatcatcaag
      421 taccagtccc accgagtcta aaaatggcat catttccatt ttcaggatca cagcgcgttg
      481 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
      541 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
      601 ttttaccgcg cgttccgata ccagttgata gttaatgatg aaaaaaaggc caataaatga
      661 cagtgctgcc aataaaaata aaattatcag ccttatcttt atcttttcgt ataaactcag
      721 catgttaata catttaacaa aataactgtc tgaacgctgt gccgctgcaa aaacagtccc
      781 ttgctgcggc tatttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac
      841 ttgcttaggg caataatttc ctgtgaggtg attactcttt caagcaatat tcaaacgtaa
      901 ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc
      961 cagtacagtc agaaaaatag ccagggccgg aaaaacagcg acatgcggcg cgataaccat
     1021 atccgctcga gcctcattga gcattgctcc ccactctggt gtcggcggct gcgcaccgag
     1081 gccgagaaat gacaggctgg cggcagagat aatcgaggta ccaatgcgca tggtgaaaaa
     1141 caccacgata gaagagacgg tcccaggcag gatatgacgc aacaaaatgg tcatatcgct
     1201 ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc
     1261 gcgcaccagg cgggcaaacg cagggatgga aaaaatggcg actgcaataa tcacgttagc
     1321 aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa
     1381 cagcacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccaa
     1441 caagcccagc aacgtcccta tcgccgcacc gataaacacg gcaaacacgc ccgccgccag
     1501 cgagatttgc gcaccaacca ggacacggct gaaaatgtcg cgccccagtg aatcgacgcc
     1561 aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc
     1621 gtcataggga gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa taacgaataa
     1681 tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt
     1741 acgtacctgg tcaggtttga ccagtggcat ggcgtttaac accgcctgac gtcgccagtt
     1801 aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc
     1861 accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc
     1921 gggtaatcgc gcatttctac ggagtcaacg agtaagcgtc caagtccggg ccagttgaaa
     1981 actttctcca ccacgatgga accaccgagc aaaaagccaa actgtaagcc catcatggtc
     2041 actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt ttcgctcacc
     2101 cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa cgacgcgcgg
     2161 gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg taaaatgtag
     2221 tgctgccaac tgtctgctcc cacggtaggc agccagccca gttcaacgga gaatacctga
     2281 attaaaagca tccccagcgc aaatgccgga aacgagatcc ccgacaccgc aatggtcata
     2341 ctcaatcgat ccggccaacg gttacgccag acggcggcga taattcccgc cgccatacca
     2401 aatatcaccg cccagaccat actggttatg gtcagccaca gcgttggcat aaagcgcctg
     2461 gcaatctcat cggcaaccgg acgacgtgac accatcgaca ggccaaaatc cccctgcaca
     2521 gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga
     2581 cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caatcaatcg cgccggatcg
     2641 ccgggcagca tatgcacaaa taaaaacacc agcaccgaga cgataaacag cgtcggaatc
     2701 aaccccagta atcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca
     2761 aatccgcgtc ttcaaagctg aagccggtat ctggcatgat ccaaaaaccg gtcaggtttt
     2821 tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgac tcttgccaga
     2881 tgatatcctg cgccgcctta tataagcggg tcttttccgc cggatcatta gttttcagtg
     2941 cctgagccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg
     3001 gtggccagtt ttgcgaggca aacaacggcg atagtgccca gtccgcttcg ccggttgaag
     3061 ccgaccagcc agtgtagaac atccgcacgc cgctctcttt ttgcccttta ccttcaactt
     3121 ctgccgcccg ctgtccggca tccatcgcag tcacctgggc tttaatcccg acctgcgcta
     3181 actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca
     3241 gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg
     3301 gatcgtaagg ccacggttta taactttgcg cgtaggcgat actcggcggt accacaccag
     3361 tagctggcgt tgcatagccc gcgaaggcca ctttcaccag cgccggacgg ttaatggcgt
     3421 aattcagcgc ctcacggacc ttcgggttat cgaatggctt ttgcgtcacg ttcatactga
     3481 tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca
     3541 gtgcggcctg ctcgtaagga atggggaaag caaactgcgc ttcaccggtt tgcagcattg
     3601 ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc
     3661 ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat
     3721 ccagttcata cggtccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca
     3781 gcgctgccgg tgaaatcatc gcggtcgccg gatgggcaag aatattaata aacgctgaga
     3841 acggctgttt gagggtaatc tttaccgttg tcggatcgat cgcttccgtt ttagcaatat
     3901 tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg
     3961 cttttaccgc cgctgcgttg aaatcggtgc catcctggaa tttaattcct tcccgcaatt
     4021 tcacggtgta agtaatgccg tcatcggaaa cggtataact ctccgccagc acgtttttca
     4081 gtttcatctc tttatccaga ccgaacagcc cctggtaaaa cgatttcgct acggcctgag
     4141 ataacgtgtc attcgcgtca tacggatcga gcgtggtgaa attcgatcct accgccacca
     4201 ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca
     4261 ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc
     4321 atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac
     4381 gagacggctg ccacctcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc
     4441 agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta
     4501 cgcgtataag gatgctgcgg gttttcgaag accgcgcgcc gtggaccaat ttcaacaatt
     4561 tgcccgagat acatcaccgc cacacgatga ctaatccgct ctaccacagc catatcgtgg
     4621 gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc
     4681 tgcccgcgaa ttgaaacatc cagcgccgaa acggcttcgt cggcaatgat cacttttgga
     4741 ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatgc
     4801 gggtaacgcc aggcatgttc aggtaacagg cccacgcgct ccagcaacca cgcaacacgt
     4861 gcaaccgctt ctttacctgg caataaaccg tgtacgcgca gcggttcgat aatcgaatca
     4921 ccgatggtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcg
     4981 cggcgcaatg cctgaagttt gccgggtgac aaggtatcga ttcgctgacc gttaaagata
     5041 atttcgccgc cctgcgattc gaccaggcgc agcaacgccc gcccggtagt ggatttaccg
     5101 ctgccagact cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa actgactttc
     5161 tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa aggaaaacgg
     5221 gtgaccagat tacgcacccg taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg
     5281 gggggggcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct
     5341 aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt gtagggatgt
     5401 tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc
     5461 accagtaccc gatcggcaat ctctgccacc acgcccatat cgtgagtgat aaagataacg
     5521 cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg
     5581 acatccagcg cggtggttgg ctcatcggct atcagcactg ccgggcggca tgacagcgcc
     5641 atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcggata acgtgaaaga
     5701 atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct
     5761 tcttcacgac tggcgttctg atgcagacga attgattcgg caatctgttc acccacagta
     5821 aatactgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcgc
     5881 acatggcgca tttgtgcagc gctctgctcg ctaagttcaa tcacatcacg actgcgccgc
     5941 cgcaacagca ttttatcgca ctgcactaaa ccgcccgcct gttccaacag acgcatcaat
     6001 gccagcgcag tcactgactt accggagccg gattcgccaa caattgccag cgtctcaccg
     6061 cgttgcagac taaaagagag attgcggacc gcagctattt tctgctggtc ctgcataaag
     6121 gcaatattaa gattttcaac cgccagcaca ttaccggcat caagttcatc actgtgtggc
     6181 aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca
     6241 ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc
     6301 gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc gctcgcaggc
     6361 ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atgtcatatg ccgccagcgc
     6421 gcggatgaag acttcgcccg tgccggtaca agaaaccgcc acactggcgt tattggcgta
     6481 gcatccggca cccactaagg ggctatcgcc aactcgtccg ggtaatttat tggtcattcc
     6541 gcccgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg cccccacggt
     6601 gcccattttc tgtttttcat ccagtggcgc accgctatgg tcgaggactg ttgccccttc
     6661 ctcgcgcgct gccattagtt gttcataacg caaaggcgtg gagaaaatct ccggtgagac
     6721 gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg
     6781 cgggctttac tccatcacca gccgggcggc aagaaccgga ttacgcagat gactaacgcc
     6841 cgccaccgca ccggctttca gggtgttacc atccatcaca caggcgtcca gttcatgcgt
     6901 ttcatcacgc gtaaagacgg cgccaattcc ggcgttaaac agtggacact cttccagcag
     6961 acgcaccgct tccgtcacca catccagcgc actttcgccc gcttccagca ttttctgccc
     7021 ggtttcaaca atggcagaca acgcctcgat gtagcgtaat tcctgttgca gactcatctg
     7081 cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt
     7141 cgtccattaa gggtggctcg ccacttttgc tataaatatc agaattttta tgaattactc
     7201 attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggacc cagcgctacg
     7261 cccgaggtgg catacagtct gtggcgtttt tctgacataa taggcaaatt cgattttgcc
     7321 tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta
     7381 atgagatgct ttctcgcgtc accccactga ccgcccagga aacgctgcca ctggttcagt
     7441 gttttggtcg tattctggcg agcgatgtcg tttcgccgct ggatgtccca gggtttgata
     7501 actccgcaat ggatggttac gcggtacgtt tggccgatat cacctccgga caaccgctgc
     7561 ccgttgccgg taaatccttt gccggtcagc cgtaccatgg tgaatggcct gcgggtacct
     7621 gcattcgtat tatgaccggt gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg
     7681 agcagactga acaaacggac aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa
     7741 atattcgccg tcgcggtgaa gatatctctg caggtgcggt tgttttcccg gcggggactc
     7801 gcctgactac cgcagagctg ccagtaattg cttcactggg gattgccgaa gttccggtga
     7861 ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg ccaggccagc
     7921 cgctgggcga cggccaaatc tacgatacca accgtctcgc cgtacacctg atgttagaac
     7981 agctgggttg cgaggtaatc aacttaggga ttatccgcga cgatccacat gccctgcgcg
     8041 ccgcatttat tgaagccgac agccaggcgg atgtggtgat cagttccggc ggtgtttcag
     8101 tgggtgaagc ggattacacc aaaacgattc ttgaagagct gggggagatc gccttctgga
     8161 agctggcgat taaaccaggt aaaccgttcg cgttcggtaa actcagcaat agctggttct
     8221 gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt
     8281 tgctggcaaa actaagcggc aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca
     8341 cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc gtgctgcacc
     8401 gcaacgccga tggcgaactg gaagtgacga ccaccggaca tcagggttca catatattca
     8461 gctcctttag cctcggcaac tgctttatcg tgctggaacg cgatcgcggc aatgtggaag
     8521 tgggcgaatg ggtggaagta gaaccgttta acgcgttgtt cggaggcctg taatggcgga
     8581 actcagcgat caagagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt
     8641 tgacggtcag gaggcgctga aagattctcg tgtgctggtc gtcggcctcg gtggactcgg
     8701 ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt
     8761 cgactcggtt tcgctctcga atctgcaacg ccagacgctg cacagtgatg ctacggtcgg
     8821 gcaaccgaag gtggaatccg cccgtgacgc tctgacgcgg atcaacccac atatcgcgat
     8881 tacgccagtc aatgcactgc tggatgacgc agaacttgca gcattgattg ctgaacacaa
     8941 tcttgtgctc gactgtacgg ataacgttgc ggtacgtaat caactgaacg caggctgttt
     9001 tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaagggc aaatcacggt
     9061 cttcacttat caggacggcg aaccgtgcta tcgctgcctt agccgtttgt ttagcgagaa
     9121 cgcgttaacc tgcgtagaag caggcgtgat ggcaccgttg atcggcgtga ttggttcgtt
     9181 gcaggcaatg gaagcgatca aactgctggc aggttatggc aaaccggcca gcgggaaaat
     9241 cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg
     9301 gtgtgaggtg tgcgggcagt aattgccacc tccaggcctg ataagacgcg acaagcgtcg
     9361 catcaggcat ttgctccaga cgccagatgc ggcgtgaacg ccttatctgg cctacggttc
     9421 gggcacagat ttccaagcgc ggcaggcatc ggtgcactcc gccacgtcat cagcaattta
     9481 tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg ccagtcctgc
     9541 tcaaacttcg ccacagcggc atcaaccgcc ggatagctaa tcatctgttg tgccacatcc
     9601 agtggcagag taattgattc acatcctgcc agtaagcagt ccagcgcctg acgcggggtt
     9661 ttgaaactcg cggctaacac tttcgcccgc ggcgcatgca ttttcaataa ctggtgtaag
     9721 tcggtcacag tctgaatacc gctaccgccc tgagcatcaa tacgattaac gtaaggcgca
     9781 acatattccg cacctgccag cgccgacagc agcccttgtg ctgcgccata taccgcggtt
     9841 cccagcgtcg gaatcccttc cgcttttaac atcttaatag ctgccagccc ctcggtggtc
     9901 accggaactt tcaccacgat atccgcaata atagaacgca atttacgtgc gtcattcacc
     9961 atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc gcccatcgct
    10021 tcatgaagtt ccggaagcac aacctccagc ggttttttac ccgcggcgat aatgcttggg
    10081 ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct
    10141 gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc tgaatgtaga
    10201 ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc attcgaaagt
    10261 attttaatat ttgaacgaaa cagagaggca actatgattt tcaatattca gcgctactcg
    10321 acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc gctgggctgc
    10381 cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta tgacccacgt
    10441 ctgtgcctgg aaggctgtga gctgtgcgct aagaccgcgc cggaagtgat tgagcgcgcg
    10501 ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc
    10561 gactgctgcc cgacacagtc gttaaccgtg tgtggggaag tgaaaagcgt tgaggagatc
    10621 atggtgaccg ttctgcgcga taaaccgttt tacgatcgca gcggcggcgg cttaacactt
    10681 tctggcggtg aaccattcat gcagccggaa atggcgacgg cgctactgca agccagccac
    10741 gaggccggca ttcatactgc ggtagaaacc tgcctgcatg tgccgtggaa atatatcgcc
    10801 ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc cgacgcgccg
    10861 tttaaacagt ggaccgacgg taacgccgcc agggtgctgg ataacctgaa aaaactcgcc
    10921 gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc aaggctttaa tgccgacgaa
    10981 acctctgtga aagccattac cgattttgcc gccgacgagc tgcacgttgg cgaaattcat
    11041 ttcctgccct accacacgct gggcatcaac aaatatcact tacttaatct gccctatgac
    11101 gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca gtatgcctgc
    11161 cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg aaactggaca
    11221 cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg aaaccgccgg
    11281 tttgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc gataagccga
    11341 tccctgtacg tcgcgcgctg gcgctggcac atcacctggc gaatcgcact atctggatca
    11401 agcacgatga gttgatcatc ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc
    11461 cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat cgtcccggtg
    11521 ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc ccgtggtggc
    11581 gcggtcagac ggtacaggat cgctgctacg gcatgtttac cgatgagcaa aaaggtctgc
    11641 tggcgaccgg aatcattaaa gcggaaggca atatgacctc cggcgatgcg cacctggcgg
    11701 tgaatttccc gctgctgctg gaaaaaggct tgatggtctg cgcgagaaag tagcggaacg
    11761 tcgctcgcgc atcaatctga cggtactgga agatctgcac ggcgaacagt tcctgaaagc
    11821 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    11881 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    11941 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    12001 ttacttcatc cagttgattt tgcagatcga atctaacggt cactcagtat cgtttggtcg
    12061 tatggaccag tatctctacc cgtactatcg ccgcgacgtt gaactcaacc agacactgga
    12121 tcgcgaacac gccatcgaat tgctgcatag ctgctggctg aaactgctgg aagtgaacaa
    12181 aatccgctca ggctcacact caaaagcctc tgcgggaagt cctctgtatc agaacgtcac
    12241 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    12301 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    12361 ccacgcggga atgagcaacg atttcctcga cgcctgcgta caggtgatcc gttgcggctt
    12421 cgggatgccg gcgttcaaca acgacgaaat cgtcatcccg gaatttatta aactcggtat
    12481 tgaaccgcag gatgcttaca actatgcagc gattggttgt atcgaaaccg ccgtcggtgg
    12541 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    12601 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    12661 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    12721 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    12781 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    12841 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggttg gcattgccaa
    12901 cctcggcaac agcctggcgg ccatgaagaa actggtattt gaacagggcg cgattagtca
    12961 gcaacagctg gctgccgcac tggcgaatga cttcgacggc ctgactcacg agcagctgcg
    13021 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgatactg tcgatacgct
    13081 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccgcgcta
    13141 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    13201 accgtttggc gcgcaaacta tggcaacgcc agacggacgt aaagcccaca ccccgctggc
    13261 agaaggcgca agcccggctt ccggtactga tcatcttggc cctactgcgg tcattggctc
    13321 ggtgggtaaa ctgcctacgg cagcgattct cggcggggtg ttgctcaacc agaaactgaa
    13381 tccagcaaca ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgcacctt
    13441 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    13501 ggaggcgaaa aaacatcccg atcagtatcg cgatctggta gtgcgtgtcg cgggttattc
    13561 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtaccgaaca
    13621 tatgctgtaa tcccctcagc ccggcggaga cgtcgccggg ctaaatcatt cacatcatca
    13681 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    13741 tgttacggtt tcattttcga gccgggagca cacctatgag cgtaaaagtt atcgtcacag
    13801 atatggacgg tacttttctt aacgacgtca aaacgtacaa ccaaccacgt tttatggcgc
    13861 aatatcagga actgaaaaag cgcggcattg agttcgttgt cgccagcggt aatcagtatt
    13921 accagcttat ttcattcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    13981 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    14041 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    14101 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    14161 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    14221 tttcactcaa cctgccggat gaacaaatcc cgttagtgat cgacaaactg cacatagcgc
    14281 tcgatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    14341 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    14401 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    14461 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    14521 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt actggataac acatcccctt
    14581 ttaacagctg acctctctac cggagccatg cgctccggtt atcccttctt tttacatcct
    14641 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttata ttgtcacatg
    14701 caaatcagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    14761 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    14821 gcgcagcttg gcgacgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    14881 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    14941 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    15001 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    15061 ctggactatc tggaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    15121 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    15181 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    15241 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    15301 cgtatccgcc gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    15361 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgctaa cgtctttaat
    15421 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    15481 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gacagcgctc
    15541 tctgatatgc tgcctagtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    15601 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    15661 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    15721 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    15781 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    15841 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    15901 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    15961 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaattgaa
    16021 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    16081 ctttcttaat gcctgataag catagcgcat caggcaatct tgcgtttgtc atcagccgcc
    16141 gtcaccccga cagtatcggg gtgacggata ataacttact cgatcccttt actacgcagg
    16201 taatcttcgt aattaccgct aaagtcgatc acgcgttccg gggtgatttc cagaatgcgg
    16261 gtcgccaggg agcttacgaa ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac
    16321 agttccagcg ccatgttcag tgactcgatg gattccatat ccaggtggtt ggtcggttcg
    16381 tccatgatca gaatgttcgg cttctgcatc attaacttac caaaaagcat ccgccctttt
    16441 tctccaccgg aaagcacttt agcaggcttt ttgatgtcgt cctggctgaa cagcaaacga
    16501 ccgagaatac tgcgcaccgc ctgctcgtca tcgccttcct gcttccactg gctcatccat
    16561 tcgaacacgg tcagatcatt ttcaaactca tattcgtggt cctgagcata gtaaccaatg
    16621 cgcgcgtttt cagaccattt tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt
    16681 ttcaacagcg ttgatttacc gacgccgttg gtacccagta ccgccagttt ttcaccgact
    16741 tccagcagca ggttgagatt tttaaacagc ggaccgttat caaacccttt ggtcagacct
    16801 tccacttcca gcgcgttacg gaacagcttc ttatcctgtt caaaacggat gaacgggttc
    16861 tgacggctgg aggctttcac ctcttccagt ttgattttat caatctggcg cgcgcgcgaa
    16921 gttgcctggc gagatttcga ggcgttggcg ctaaagcggc taacgaaaga ttgcagctca
    16981 gcaatctgcg ctttcttctt ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc
    17041 gccgtcatgt actcatcgta gttacccgga taaacgcgca gctcgccgta atccagatcc
    17101 gccatgtggg tacagaccat gttaaggaag tgacggtcgt gcgagatgat gatcatggtg
    17161 ctgtcacgct cgttcagcac ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg
    17221 gtcggttcgt cgagcaggag aatatccgga tcagcaaaca gcgcctgcgc cagcaacaca
    17281 cgcagcttcc agccaggagc aacttcactc atcgggccgt agtgctgttc cactggaatt
    17341 cccacgccaa gcaacaattc accggcacga gcttccgcag agtaaccgtc catttcgccg
    17401 tatttaactt ccagatcggc cactttatag ccgtcttctt cactcatttc cggcaaagca
    17461 tagatgcggt cgcgctcctg cttcacttcc cacaactctt tatgccccat gatcaccgta
    17521 tccagcacag tgaactcttc aaaggcaaac tgatcctgac gcagtttacc aatgcgctcg
    17581 ttgggatcga gggaaacgtt acccagcgtc ggctcaaggt cgccgccgag gatcttcata
    17641 aaggtggatt taccactacc gttcgcgcca atcaggccgt aacggttgcc gccgccaaat
    17701 ttgacggaaa tgttttcaaa caacggctta ctgccgaact gcatggtgac gttactggaa
    17761 actaacacag gcgtatcctg aaaagagata tgacaaaccg cgcattatgc cataagtctg
    17821 aaagaagatc ctgctcctcg cttaagatct acactcaagc caaaggcaaa aaatgtgatt
    17881 tcgtacacat ctgatttcac tgtgagctgg aatgaactta taatgcgctt ccaatactct
    17941 aatattctca acccaatggc ctgccaggca caaaatctcg cttaacatga atatgaaatt
    18001 gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt
    18061 aacttatcct ctgccaaccg acgggagtcg cctggttggt cagaatcagg tgatcaccat
    18121 tcctgaaggt aacactcagc cgctggagta ttttgccgcg gagtaccaga tggggctttc
    18181 caatatgatg gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg gtactgtact
    18241 gaacattccg cagcagctga tcctgccgga taccgttcat gaaggcatcg tcattaacag
    18301 tgcagagatg cgtctgtatt actatccgaa agggaccaac accgttatcg tgctgccgat
    18361 cggcattggt cagttaggca aagatacgcc tatcaactgg accaccaaag ttgagcgtaa
    18421 gaaagcaggc ccgacctgga cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg
    18481 cgaaccgctt ccggctgtcg ttccggcagg tccggataac ccgatggggc tgtatgcact
    18541 ctacatcggt cgcctgtacg ctatccatgg caccaacgct aacttcggta tcggtctgcg
    18601 tgtaagtcat ggttgtgtgc gtctgcgtaa cgaagacatc aaattcctgt tcgagaaagt
    18661 actggtcggt acccgcgtac agtttattga tgagccggta aaagcgacca ccgagccaga
    18721 cggcagccgt tacattgaag tccataaccc gctgtctacc accgaagccc aatttgaagg
    18781 tcaggaaatt gtgccaatta ccctgacgaa gagcgtgcag acagtgaccg gccagccaga
    18841 tgttgaccag gttgttcttg atgaagcgat taaaaaccgc tccgggatgc cggttcgtct
    18901 gaattaatct tcaaaacgta aaacaaaggc ggactcataa tccgcctttt ttatttgcca
    18961 gaccttacca ggccgggatc ataaccaaca aaacatatcc caccagcgcc gcccacaaca
    19021 gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc gccatacgta
    19081 gcgcaatcaa atttgccagc gatccgggta ataacccaaa gccacctacg tttaccgccc
    19141 atgccagtaa taaagacggc ggcacatagt tcagcaacaa tatagtactc ggcacgttac
    19201 tgatcacctg cgataaaccg attgccgtta accataatcc gggttcagat aaatgactca
    19261 cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg tcgataaaca
    19321 tcgccataaa caccagcagc agcgtccagt ccacactcaa caccacgcga cgcgccagca
    19381 gcgcaaagcc cgccgccaca atcaccagtc cccacagctc ttgtttgaac tccagcgccg
    19441 tcagaaagac gatatacagc cccagacaac tccacaccag ccgcggtttc cactccggtg
    19501 tttgcacccc cgtatggtat tggagtgcct ttccagggaa acaacaccag cacaacagca
    19561 ggagcgtcag catcattgcg ccagccagcg gtgccatttg ggcaataaat ccggcaaacg
    19621 aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg
    19681 aaccagcgtt gactgccagc gcctcaaaaa taatcagccg attaaccggg atctcacaca
    19741 gcctttttag cgtgatagtc agcggaacaa caataaacag cgtgacatcg ttggtcagaa
    19801 aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa
    19861 agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg
    19921 tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca gcaggccagg
    19981 atttcggtgc aaagggcacg aaaaaactta atccgatacc aacaagaatt aataactgaa
    20041 aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg
    20101 ggtaaacaaa cgaaaggcat ccagcgtctc ttcactaaca tggtgctcca tgccttccgc
    20161 gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt cgactatctg
    20221 atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa acacgccacg
    20281 ccaggggatc atttcaatca gccccatagt tgccagtcgc ttaagcattt tagccaccgt
    20341 cggttgcgaa actcccaaac gagcagccat gtctacctga cgagcttccc ccacttccct
    20401 gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat gcgcctctct
    20461 gacctggcgg aacccttcaa cgtgctcttc ctcgttcact aactgcgtca ctttttttgc
    20521 tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc
    20581 gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt
    20641 tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa
    20701 ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg
    20761 tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg
    20821 tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc ggtacgccgc
    20881 ttcactcctg ctttcatgca ggcacgacgc gttttggtct gaaaaacccc actttttgtc
    20941 ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga actgcggctc
    21001 gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct
    21061 cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg gcgtttgtta
    21121 cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt
    21181 agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttac
    21241 cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct atggcgaaca
    21301 ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc ttgttatttc
    21361 ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt gcactccacc
    21421 aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta cctggtcggc
    21481 ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg
    21541 cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct
    21601 cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg ctatccggta
    21661 tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg
    21721 atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa gccgaagtat
    21781 cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc
    21841 ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt tgctgactac
    21901 attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat
    21961 ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc
    22021 tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc
    22081 aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg
    22141 aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg
    22201 cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgaccg gacaaaaggc
    22261 aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac tatgacgcaa
    22321 acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag cagcttctcg
    22381 ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac
    22441 cttgcccatg atgataagta tcagcaaaat ttccaggtgc cttttatggt catttccagc
    22501 gacgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt cttaggcttt
    22561 ttctcgcagt ggacgggaat taaagcgaag gaaattaaca tcaaataccc gtttatatct
    22621 gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt ggattacaac
    22681 catctcggaa ccgatatttt cgacccgaag ccttaaaaac aaaaatccgc cccgagaggc
    22741 ggatttttta tatcaccaaa gtgattagaa gcggtaacca acaccggcaa tccaggtgcc
    22801 tacgtcaacg ctacgaatac ggctctgctc gtaagagaag tccagagcaa cgttttccat
    22861 cgggttgaac tgcagaccag caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta
    22921 ggtcgggtat tcagtggtct ggaatttacc ataacccaca cccactacac cgtagatgct
    22981 tgcccagtcg ttaatgcggt aagccggacc agcagtgatg ccgtagtact ggtttttgtt
    23041 gtagtcacca gagcttgcag tacggctttt ctcggtgtaa gtgaatgaac cgatcacacc
    23101 cagcgggctg ttgtcttctt catagcggta tttcaggttg aaaccgccca ttttgttcat
    23161 ttggccctga gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg cagctacgga
    23221 agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa tttttttcat
    23281 aaccacctca aatgtgattc aaataagtcc taagttttaa atatatcaaa aattaatggg
    23341 aaactcttcg cgatttgtga tgtctaacgg gccatttcat gtaacagaac gtttccatac
    23401 accgctatcc atctaaattt aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt
    23461 tttccctgtc attcatccag attattccta atcaccagac taatgattcc atcaatcctg
    23521 gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca gcattgggta
    23581 tatccagtac actccacgct ttacttaagt ctagatattt gtgggagaaa ggatgcctgg
    23641 ttcattacgt aaaatgccgg tctggttacc aatagtcata ttgctcgttg ccatggcgtc
    23701 tattcaaggt ggagcttcgt tagctaagtc actttttcct ctggtgggcg caccgggtgt
    23761 cactgcgctg cgcctggcat taggaacgct gatcctcatc gcgttcttta agccatggcg
    23821 actgcgcttt gccaaagagc aacggttacc gctgttgttt tacggcgttt cgctgggtgg
    23881 gatgaattat cttttttatc tctctattca gacggtaccg ctgggtattg cggtggcgct
    23941 ggagttcacc ggaccactgg cggtggcgct gttctcttct cgtcgcccgg tagatttcgt
    24001 ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta ccgctggggc aagacgtttc
    24061 ccatgtcgat ttaaccggct gtgcgctggc actgggggcc ggggcttgtt gggctattta
    24121 cattttaagt gggcaacgcg caggagcgga acatggccct gcgacggtgg caattggttc
    24181 gttgattgca gcgttaattt tcgtgccaat tggagcgctt caggctggtg aagcactctg
    24241 gcactggtcg gttattccat tgggtctggc ggtcgctatt ctctcgaccg ctctgcctta
    24301 ttcgctggaa atgattgccc tcacccgttt gccaacacgg acatttggta cgctgatgag
    24361 catggaaccg gcgctggctg ccgtttccgg gatgattttc ctcggagaaa cactgacacc
    24421 catacagctg ctggcgctcg gcgcaattat cgccgcttca atgggttcta cgctgacagt
    24481 acgcaaagag agcaaaataa aagaattaga cattaattaa atttacattt ctgcatggtt
    24541 atgcataacc atgcagaatt tctcgctact tttcctctac accgtcttta tatatcgaat
    24601 tatgcaaaag catatttatt ccgaaaattc ctggcgagca aataaataag aattgttctt
    24661 atcaatatat ctaactcatt gaatctttat tagttttgtt tttcacgctt gttaccacta
    24721 ttagtgtgat aggaacagcc agaatagcgg aacacatagc cggtgctata cttagtctcg
    24781 ttaattactg ggacataaca tcaagaggat atgaaattat gagtaccgct aaattagtta
    24841 aatcaaaagc gaccaatctg ctttataccc gcaacgatgt ctctgacagc gagaaaaaag
    24901 caacagtaga gttgctgaat cgccaggtta tccagtttat tgatctttct ttgattacca
    24961 aacaagcgca ctggaacatg cgcggcgcta acttcattgc cgtacatgaa atgctggatg
    25021 gcttccgcac tgcactgatc gatcatctgg ataccatggc agaacgtgca gtgcagctgg
    25081 gcggtgtagc tctggggacc actcaagtta tcaacagcaa aaccccgctg aaaagttacc
    25141 cgctggacat ccacaacgtt caggatcacc tgaaagaact ggctgaccgt tacgcaatcg
    25201 tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga tgacgacacc gcagatatcc
    25261 tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt tatcgagtct aacatcgaat
    25321 aaatccatcg ctgatggtgc agaattttag tacccgataa aagcggcttc ctgacaggag
    25381 gccgttttgt tttgcagccc gcctcaacgc acttatttag tgcatccatc tgctatcccc
    25441 agctgattaa gtaaattttt tgtatccaca tcatcacaca atcgttacat aaagattgtt
    25501 ttttcatcag gttttacgct aaataatcac tgtgttgagt gcacaatttt agcgcaccag
    25561 tttggtgccc cagaatggtg catcttcagg gtgttgccct ataaatcgtg catcacgttt
    25621 ttgccgcatc tcgaaaaatc aatgagttgc aaaactggca cgattttttc atatatgtga
    25681 atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta
    25741 aaagtttcac tggctgcact gaccctggct tttgcggttt cttctcatgc cgcggataaa
    25801 aaattagttg tcgcgacgga taccgccttc gttccgtttg aatttaaaca gggcgataaa
    25861 tatgtgggct ttgacgttga tctgtgggct gccatcgcta aagagctgaa gctggattac
    25921 gaactgaagc cgatggattt cagtgggatc attccggcac tgcaaaccaa aaacgtcgat
    25981 ctggcgctgg cgggcattac catcaccgac gagcgtaaaa aagcgatcga tttctctgac
    26041 ggctactaca aaagcggcct gttagtgatg gtgaaagcta acaataacga tgtgaaaagc
    26101 gtgaaagatc tcgacgggaa agtggttgcg gtgaagagcg gcactggctc cgttgattac
    26161 gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc cgaacatcga taacgcctat
    26221 atggaactgg gcaccaaccg cgcagacgcc gttctgcacg atacgccaaa cattctgtac
    26281 ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag gcgactctct ggaagcgcag
    26341 caatacggta ttgcgttccc gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg
    26401 ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct acaaaaaatg gttcggtact
    26461 gaaccgaaat aataacgcta cacctgtaaa gcgcactggc agttccctct cccctctggg
    26521 gagaggatta gggtgagggc gcaaacccgc tccggggcca ttaattaccc tgaatttgat
    26581 tatttacacc acggtaacag gaacaacata tgcagtttga ctggagtgct atctggcctg
    26641 ccattccgct tctgattgaa ggtgccaaaa tgaccctgtg gatatcggtc ctcggtctgg
    26701 ctggcggcct ggtgatcgga ttgctggcag gttttgcacg caccttcggt ggttggatag
    26761 ccaaccacgt cgcgctggtt tttattgaag tgatccgcgg cacacctatc gtcgtccagg
    26821 tgatgtttat ctattttgcc ctgccgatgg cgtttaatga cttacgcatc gacccattta
    26881 ctgcggcggt ggtcaccatc atgatcaact ccggcgcgta cattgcggaa atcacgcgtg
    26941 gtgcggtgct gtctatccac aaaggttttc gtgaagcagg actggcgctc ggtctttcac
    27001 gttgggaaac cattcgctac gtcattttac cgctggcact gcgtcgtatg ctgccgccgc
    27061 tgggtaacca gtggatcatc agcattaaag acacctcgct gtttattgtg atcggcgtgg
    27121 cggaactgac ccgtcagggg caagaaatta ttgccggtaa cttccgcgcc cttgagatct
    27181 ggagcgccgt ggcggtgttc tatctgatta ttaccctggt gctgagcttt attctgcgtc
    27241 gtctggaaag aaggatgaaa atcctgtgat tgaatttaaa aacgtctcca agcactttgg
    27301 cccaacccag gtgctgcaca atatcgattt gaacattgcc cagggcgaag tcgtggtgat
    27361 tatcgggccg tccggttccg gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga
    27421 aatcacctcc ggcgatctga ttgtcgatgg cctgaaggtt aacgatccga aagttgacga
    27481 gcgcctgatt cgccaggaag caggtatggt gttccagcag ttttacctct tcccgcatct
    27541 gacggcgctg gaaaacgtca tgtttggccc gctacgcgtg cgtggcgcga acaaagaaga
    27601 ggcggaaaaa ctggcacgtg agctgctggc gaaagtcggt ctggcagaac gtgcacatca
    27661 ctacccttcc gaactttctg gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc
    27721 ggtgaagccg aaaatgatgc tgtttgatga accgacttcc gctcttgacc cggaactgcg
    27781 ccatgaagtg ctgaaggtta tgcaagatct ggctgaagaa gggatgacga tggtgatcgt
    27841 gacccacgaa atcggttttg ccgagaaagt agcttcgcgc ctgatcttta tcgacaaagg
    27901 ccggattgcg gaagatggcg atccacaagt gttgattaaa aatccgccga gccagcgctt
    27961 gcaggaattt ttacagcacg tctcttaact tctctgcctc cctttcaccc gaaagggagg
    28021 tacaccagat tcctctcatt taaaatcacc cctcccccag catctatact tatctttttg
    28081 ctctgttttc tcactggagg agtcatgcgg tggatcctgt tcatcctctt ctgcctgctg
    28141 ggcgcacctg cccacgcggt atccataccc ggcgttacaa ccacaacgac aacggactca
    28201 acgactgaac cggccccgga accggatatc gaacaaaaaa aagcggctta tggcgcactg
    28261 gcggatgtgc tggataatga cacctcgcgt aaagagttga tcgaccagtt gcgcaccgtc
    28321 gccgcgacgc cacctgctga accggtcccg aagatcgtgc cgccgacgct ggttgaagag
    28381 caaaccgtgc tgcaaaaggt caccgaattc agccgccatt atggtgaagc cctttccgcc
    28441 cgcttcgggc agctttatcg caatatcacc ggctccccgc ataagccgtt taatccacaa
    28501 accttcagca atgcgctgac ccatttttca atgttagcgg ttttagtgtt tggtttttac
    28561 tggctgattc gcctgtgcgc actgccgctg tatcgcaaaa tgggtcagtg ggcgcggcaa
    28621 aaaaatcgtg agcgcagtaa ctggctgcag cttccggcga tgattatcgg ggcgtttatt
    28681 atcgacctgc tgttactggc actgacatta tttgtcggcc agatattaag cgacaacctg
    28741 aatgcaggca gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg
    28801 attgaatttt tcaaagccgt actacgcctg attttttgcc caaacgtggc ggaactgcgc
    28861 ccgttcacga ttcaggacga gagcgcccgt tactggagtc gtcgcctgag ctggttaggc
    28921 agtttgatag gttatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta
    28981 cagataggtg cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac
    29041 ctgatctttc gtaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg
    29101 ctggcctttt tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc
    29161 gcctatttta tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag cctgaaattt
    29221 atgatgggtg caacagtgcg cagcctggcg attattggta tcgcagcatt tgtttccggt
    29281 atgttttccc gctggctggc aaaaaccatc actctctcgc cacatactca gcgtaactat
    29341 ccggagttgc aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt
    29401 ctgaccgtct gcgtggcggt aatgctgctg ttgagcgcat ggggattgtt cgatttctgg
    29461 aactggctgc aaaacggcgc ggggcagaaa accgtagata tcctgatccg tatcgcactc
    29521 attcttttct tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg
    29581 gcttcggata ttcatggccg cccgctaccc agcgcccgca cgcgtaccct gcttacgctg
    29641 tttcgtaacg cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa
    29701 atcggcgtca atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg
    29761 tttggttcgc aaacgctggt gaaagatatt atcaccgggg tatttattca gtttgaaaac
    29821 ggcatgaaca ctggagattt ggtgactatt gggccgttga ccggcactgt ggaacggatg
    29881 tcgattcgct ccgtgggcgt acgccaggat accggggcgt accacatcat tccgtggtct
    29941 tcgattacca cctttgccaa ctttgtccgc ggcattggtt cggtagtggc aaactacgat
    30001 gttgatcgcc atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtagcggaa
    30061 ttaatggaaa acgaagaaat tcgtgggctg attattggtg aaccgaactt tgccgggatt
    30121 gttggcttaa gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa
    30181 cagtggacgg tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc
    30241 gttcgcgcgc cggtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa
    30301 ccgttaccgc cgggggagcc aacgctttaa cgctggcgat tgacaaaacg gcggcgctgc
    30361 tcgtcgttca taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct
    30421 tttttaacca ccttcaccgc gcccacgtcc gtcagggcac gatacaacgg cggtaagttt
    30481 tcaccacgag ataccagtga agtaaaccac atcacctgct tcgcgaagcc tttgctctct
    30541 tcaatcatct ttttgataaa ggtgacctca ccgccttcac accacaactc ctgttgctgg
    30601 ccgccaaagt tcagtgcatc gtctttgttc agccccaggt tacggcgttt acactcacta
    30661 cctgcccgcg ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat
    30721 tgctcgtttt tatggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga
    30781 cgaatggcgc gattaagccc cggattagca ctgatgatcg cctgcgcgct gcttaacgcc
    30841 tggctgctgg tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga
    30901 taaatacagt tcgcaccaac gccgatatca agaatgctgg cattagctgg aattgttccg
    30961 cttgcttcag ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggc
    31021 gggcagagaa aaccgtccgg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac
    31081 gccttattga gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctccccggcg
    31141 ggtgtaagcg tgaggaattg cttgagttca ggattgacct gacaaagcgt ggcgagatcg
    31201 tagcggctgt gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc
    31261 tcctttaagc agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt
    31321 ttatcattcc ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag
    31381 cattatgcat gtcattgatt tgtcttattt tgtaaaaata tatcgttcaa aaaataagca
    31441 catctctgtc aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac
    31501 tacccgttgt atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac
    31561 cgtcctgagc ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag
    31621 cggtcagcta cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct
    31681 ggaggataaa ctggcagaaa aagcgcgcga gcaaggcgcg aaaggttatg tcattaattc
    31741 cgcaggcgga aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg
    31801 taactgcatt cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata
    31861 tgaacgcaat gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgcc
    31921 aagtgataat gcttatc
//
LOCUS       DAZFKY010000039        31180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFKY010000039 DAZFKY010000000
VERSION     DAZFKY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..599
                     /locus_tag="ACHKCL_001567"
     CDS             <1..599
                     /locus_tag="ACHKCL_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5762564.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            829..1212
                     /gene="secE"
                     /locus_tag="ACHKCL_001568"
     CDS             829..1212
                     /gene="secE"
                     /locus_tag="ACHKCL_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5762565.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1214..1759
                     /gene="nusG"
                     /locus_tag="ACHKCL_001569"
     CDS             1214..1759
                     /gene="nusG"
                     /locus_tag="ACHKCL_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5762566.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1918..2346
                     /gene="rplK"
                     /locus_tag="ACHKCL_001570"
     CDS             1918..2346
                     /gene="rplK"
                     /locus_tag="ACHKCL_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5762567.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2350..3054
                     /gene="rplA"
                     /locus_tag="ACHKCL_001571"
     CDS             2350..3054
                     /gene="rplA"
                     /locus_tag="ACHKCL_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5762568.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHKCL_001572"
     CDS             3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHKCL_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5762569.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3910..4275
                     /gene="rplL"
                     /locus_tag="ACHKCL_001573"
     CDS             3910..4275
                     /gene="rplL"
                     /locus_tag="ACHKCL_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5762570.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHKCL_001574"
     CDS             4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHKCL_001574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5762571.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHKCL_001575"
     CDS             8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHKCL_001575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5762572.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13136..13579
                     /locus_tag="ACHKCL_001576"
     CDS             13136..13579
                     /locus_tag="ACHKCL_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO5762573.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHKCL_001577"
     CDS             complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHKCL_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5762574.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHKCL_001578"
     CDS             complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHKCL_001578"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5762575.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHKCL_001579"
     CDS             complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHKCL_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5762576.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHKCL_001580"
     CDS             complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHKCL_001580"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5762577.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHKCL_001581"
     CDS             complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHKCL_001581"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5762578.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHKCL_001582"
     CDS             complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHKCL_001582"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5762579.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19459..19559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHKCL_001583"
     CDS             complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHKCL_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5762580.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20182..20955
                     /gene="nudC"
                     /locus_tag="ACHKCL_001584"
     CDS             20182..20955
                     /gene="nudC"
                     /locus_tag="ACHKCL_001584"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5762581.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20995..22059
                     /gene="hemE"
                     /locus_tag="ACHKCL_001585"
     CDS             20995..22059
                     /gene="hemE"
                     /locus_tag="ACHKCL_001585"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5762582.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22069..22740
                     /gene="nfi"
                     /locus_tag="ACHKCL_001586"
     CDS             22069..22740
                     /gene="nfi"
                     /locus_tag="ACHKCL_001586"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5762583.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHKCL_001587"
     CDS             22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHKCL_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5762584.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23560..23832
                     /gene="hupA"
                     /locus_tag="ACHKCL_001588"
     CDS             23560..23832
                     /gene="hupA"
                     /locus_tag="ACHKCL_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5762585.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHKCL_001589"
     CDS             23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHKCL_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5762586.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHKCL_001590"
     CDS             complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHKCL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5762587.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25205..26581
                     /gene="zraS"
                     /locus_tag="ACHKCL_001591"
     CDS             25205..26581
                     /gene="zraS"
                     /locus_tag="ACHKCL_001591"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5762588.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26578..27903
                     /gene="zraR"
                     /locus_tag="ACHKCL_001592"
     CDS             26578..27903
                     /gene="zraR"
                     /locus_tag="ACHKCL_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5762589.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHKCL_001593"
     CDS             complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHKCL_001593"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5762590.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHKCL_001594"
     CDS             complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHKCL_001594"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5762591.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7321 a         8072 c         8536 g         7251 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
      601 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
      661 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
      721 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
      781 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
      841 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
      901 ttgctcttgg tggcgattgt cggcaactat ctttatcgcg acattatgct gccgctgcgt
      961 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     1021 ggtaaagcta ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     1081 ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     1141 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     1201 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     1261 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     1321 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     1381 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     1441 cgacgcgagc tggcacctgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     1501 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     1561 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     1621 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     1681 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     1741 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     1801 cgcgagattg gaatacaatt tcgcgccttt tgtttttatg ggtcttgccc gtaaaacgat
     1861 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     1921 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     1981 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     2041 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     2101 tacgctgacc gttctttcac tttcgttacc aagactccgc cggcagcagt tctgctgaaa
     2161 aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     2221 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     2281 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     2341 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca
     2401 accaaacagt acgacatcaa cgaagctatc gctctgctga aagagctggc gactgctaaa
     2461 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     2521 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     2581 tttacccagg gtgcaaacgc tgaagctgct aaagctgcag gtgctgaact ggtaggtatg
     2641 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     2701 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg cggcctgatg
     2761 ccaaacccga aagtgggtac tgtaacgccg aacgttgctg aagcggttaa aaacgctaaa
     2821 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     2881 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     2941 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     3001 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     3061 tacgtgggcg gtgattttgt ctacaatctt acccccacgt atgctaacga aagttagcag
     3121 ctaaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag
     3181 tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta
     3241 tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag
     3301 tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt
     3361 caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca
     3421 gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt
     3481 cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa
     3541 ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct
     3601 atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa
     3661 tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc
     3721 ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa
     3781 gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct
     3841 taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga
     3901 acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta
     3961 atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct
     4021 gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta
     4081 attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact
     4141 ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa
     4201 ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa
     4261 gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg
     4321 tgacttttta gtcaccagcc tttttgcgct gtaaggcacc agtagcgttt cacactgttt
     4381 gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga
     4441 cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg
     4501 aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca
     4561 gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa
     4621 acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc
     4681 tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct
     4741 ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca
     4801 atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg
     4861 cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc
     4921 gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct
     4981 catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct
     5041 gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa
     5101 agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga
     5161 tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat
     5221 tctgcgtgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat
     5281 ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga
     5341 aaccgcatcc tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat
     5401 cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt
     5461 tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct
     5521 gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg
     5581 tcacaagcgt atcgaaacgc tgttcaccaa tgatctggat cacggcccgt atatctctga
     5641 aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat
     5701 gatgcgccct ggcgagccgc cgactcgtga agcagcggaa agcctgttcg agaacctgtt
     5761 cttctccgaa gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct
     5821 gctgcgcgaa gaaatcgaag gttctggtat cctgagcaaa gacgacatca ttgatgttat
     5881 gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg
     5941 caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt
     6001 acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc
     6061 tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag
     6121 ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg
     6181 tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga
     6241 cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat
     6301 cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac
     6361 tccgtatcgt aaagtgactg acggtgttgt aactgacgaa attcactacc tgtctgctat
     6421 cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt
     6481 cgtagaagac ctggtaacct gccgtagcaa aggcgaatcc agcttgttca gccgtgacca
     6541 ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc
     6601 gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc
     6661 cgttccgact ctgcgtgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc
     6721 cgttgactcc ggtgtaactg cggttgctaa acgtggtggt gtcgttcagt acgtggatgc
     6781 ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga
     6841 catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatta accagatgcc
     6901 gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac
     6961 cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg
     7021 ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac
     7081 caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga
     7141 gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg
     7201 tatcgtttat attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggttacgcc
     7261 gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa
     7321 agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat
     7381 cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga
     7441 agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc
     7501 gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct
     7561 cgacaaattg ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa
     7621 tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact
     7681 cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat
     7741 tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg
     7801 tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga
     7861 aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat
     7921 cggtcagatc ctcgaaaccc acttgggtat ggctgcgaaa ggtatcggcg acaagatcaa
     7981 cgccatgctg aaacagcagc aggaagtcgc gaaactgcgt gaattcatcc agcgtgcgta
     8041 cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt
     8101 tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg
     8161 tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga cttctggtca
     8221 gatccgcctg tacgacggcc gcactggtga acagttcgaa cgtccggtaa ccgttggtta
     8281 catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg
     8341 ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg
     8401 tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga
     8461 aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata aaaacatcgt
     8521 ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga
     8581 gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt
     8641 cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg
     8701 tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca
     8761 aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc
     8821 cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta
     8881 tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc
     8941 gtggcgtcat ctgtgagaag tgtggcgttg aagtgaccca gactaaagta cgccgtgagc
     9001 gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc
     9061 cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact
     9121 ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggagcgtcag cagatcctga
     9181 ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaaaatgg
     9241 gtgcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag tgcgaacagc
     9301 tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta
     9361 tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg
     9421 ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg
     9481 cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac
     9541 gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg
     9601 aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca
     9661 agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga
     9721 acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgtg ggtccatacc
     9781 tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca
     9841 tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg
     9901 ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg
     9961 tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac
    10021 tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact
    10081 tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc
    10141 gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg
    10201 ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca
    10261 aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc
    10321 tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg
    10381 gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga
    10441 tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa
    10501 tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg
    10561 cggaccagat catgtacacc ggctttgcat atgcagcgcg ttctggtgca tctgttggta
    10621 tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag
    10681 ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca
    10741 aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc
    10801 tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca
    10861 gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg
    10921 ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg
    10981 cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta
    11041 aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg
    11101 ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca
    11161 tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtattgg
    11221 gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca
    11281 acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta
    11341 aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc
    11401 gtgacctggc gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac
    11461 agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg
    11521 catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca
    11581 gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg
    11641 aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg
    11701 cggtactggc aaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg
    11761 acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga
    11821 tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg
    11881 ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg
    11941 ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc
    12001 tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc
    12061 tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccacgcg
    12121 ttgcggacct gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg
    12181 gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag
    12241 acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag
    12301 gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc
    12361 tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat
    12421 accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc
    12481 tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg
    12541 aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa
    12601 cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct
    12661 ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac
    12721 gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta
    12781 ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg
    12841 caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc
    12901 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
    12961 gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc
    13021 tgaatactca taatcaatgt agagatgact aatatcctga aactgactga actaattgag
    13081 tcaaactcgg caaggattcg atactattcc tgtgtagctt tcttaaggaa cgagaatgaa
    13141 acaggaagtg gagaagtggc gaccttttaa acatccggat ggcgacgttc gtgatttatc
    13201 gtttcttgat gctcatcagg ttatctacgt tcagcatcat gaggacaagg agcctttaga
    13261 gtatcgcttt tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac
    13321 gaacgaagaa aaactatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca
    13381 gcaccgttat aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag
    13441 caacgttatt catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg
    13501 agataaggca ttttactttg ttgtgttcag ggctttcagt gaaatttttc accattgcct
    13561 aaaacttatt gagaaataag cagcttcctc agccctcaaa ataacaaaac ccaccttaag
    13621 gtgggtttcg ccagagaatt atctctggta ttcagaacgc cattaccgga ctttgccttg
    13681 accttgcgat aatcgcaggt tgcgggatgt ctgaatttct tcagtctgct gcatcctgga
    13741 agatgagaac atgtgttctt attttcgtct ctatcatagt tgagtattta ctctcttaca
    13801 atcagatctc tttcattgct caacaggcga tggcttcaga ctttgcatta cggaattttt
    13861 aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt cctgctgacg
    13921 gcactaccat aaaaaaacac aacgctttca acaaccttgc gtttttcatc ttccgtagcg
    13981 gctcggaata aattttcgtt cttacattcc gtctcatagt ctttgcgagg cgcgtcccag
    14041 atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg cggcagcaac
    14101 cgcttccggt ctgcgctcgt cgtgcggtga gaactgttcc agctcggggt gattgtcggc
    14161 atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca gcggaataac
    14221 gcgatcgcga aaccacggtg attcccgcgt ggagagtgac agttcaatct ccggtgcaag
    14281 cagccggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg acgcaggctc
    14341 aatgccgcca gtacatggac gcaggcgtgg gaaggagaca gagtagcggc tttgccagta
    14401 atgctgttgc agccatagca aatgttctgc aaccatatag cagtcaacgc gccagctgtc
    14461 ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacggc ccagccgatc
    14521 cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcagatgat ggcgggcata
    14581 agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa gttgctttaa
    14641 ctcggcgtat tccgtctccg ccagcggctg tacttccatc tgtagtgaag agaactgttc
    14701 acgcagggca gggagatgac gacgaaagta atccatcccc actttcgcct gatgttcacc
    14761 ggtgactaaa agcagatgtt caaagcccat ctcccgtata gcggcgcttt ccctggcaat
    14821 atccgcttca tccagcgttt tgcgcttgat gcggttgctc atagagaatc cgcagtacgt
    14881 gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa ctgtgttgcc
    14941 aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat agccactggc
    15001 ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg
    15061 ctctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc agtccagttg
    15121 tcgccagcga tcgctgaagg ttttcatacc gatgcctcca gaaatccggt cagcgggctg
    15181 gtggcatgag caaaataact gcggctgccc ggtccggact gaggtgccag caggcctgct
    15241 tctactgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc
    15301 gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg gctgggaacg
    15361 ccgatgccag catcgacaac caccggaact gtggcctgct ggataataat ctccagcatg
    15421 gcgcgggttt ccagcccctg attcgagcca atcggcgcgc cgagcggcat caccgctgca
    15481 cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta aggcagcacg
    15541 acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg atcgggcaac
    15601 agccagcggg cgtcagggtg aatctccaat tttaaccagt ttgtgcctaa cgcttcacga
    15661 gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt atttggcagc
    15721 agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg
    15781 acacgtttca tcgccagcgt caccagctgg ctgccggaag cgcggattgc ctctaccatc
    15841 agttgtgaag aggcgaattt cccggtgccg gtaaacagat gtgaatcaaa cgttttgtcc
    15901 gcaatacgta acatctcaac cccctgcaat aacctgaaaa agtaggatct ggtcgccatc
    15961 ctgcacgata tgttgcgtcc actgctcacg cgggacgatt tgctgattaa tcgccagagc
    16021 cgcgcccgct tgtcgttgaa cgagttgctc cagtagttcg tgaacagttt gcccggcggc
    16081 gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat accgggcaac
    16141 cactggcgcg gcgcaacgcc aggctgcgcc actggctcga cttaccgtcg aacagtcgga
    16201 gttctcccgc aggcgtctct ataccgctta ataacttaat ggcttccagt gcctgcaaag
    16261 tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg cgttctggct
    16321 cctggttttc tggccacagg cagcggtaac aaccctgccc ccagggcggc gtcagtacca
    16381 tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg agtgccacgc
    16441 aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc accacatcgg
    16501 cttgtgcaac cgcatctttt aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt
    16561 gaatatcggg attcaactgt gtcagtcgct gatggctgac ctgcgatttc gggcgatcga
    16621 tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat
    16681 ctgccagtac cagcgtcccg acgccagcgc ccgccaggta tagcgcagca ggtgtaccca
    16741 gcccgccaag accgataatc agcacctggc tgtcgagcag tttttgctgc ccgtcaagag
    16801 cgatatcgtc gagcaggatt tggcggctat aacgcataaa atcacggtca ttcatcgcca
    16861 actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg
    16921 gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga
    16981 ctgataccgc caatcgccac ggtgggataa tccgacagtc gctcaacatg ccgtgccagc
    17041 tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc
    17101 agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc gtcatgggtc
    17161 gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc ttgcaaatct
    17221 tcctgcccca aatggacgcc atacgcctga tgcttgatcg cgagccgcca gtaatcgttg
    17281 ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac atcggcttcc
    17341 acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc tgcatccaac
    17401 agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg aaaaggtaca
    17461 ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt tctccgcctc
    17521 tggcacggaa gttctccgac atatccgcta ttcccatttc aatagtttgc gtggcggcgt
    17581 aatcacgcac ttcctggctg attttcatcg agcagaattt cggcccacac atggagcaaa
    17641 aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg cgggcggtaa
    17701 acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca
    17761 tggcgttatc gcgaatttgt gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg
    17821 caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga cccagatgct
    17881 cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc gccgcaccaa
    17941 tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc cccagagtgt
    18001 aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg atcatctgca
    18061 tcggcacgtg gcctgggcct tcaatcatca cctgcacatc atattcccag gcgatttttg
    18121 tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg tcctgaatag
    18181 aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca cagatttcac
    18241 aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg caccatttcg
    18301 ccataatcga accgccgcgc gagacgatac cggtcaggcg tttcgcggtc atcggcacat
    18361 agcgcagcag cacccccgca tggatagtga agtaatccac accttgctcg gcctgttcca
    18421 gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccatta accttctcca
    18481 gcgcctggta gatcggcact gtaccaatcg gcaccgggct gttacgcaaa atccactcgc
    18541 gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct ccccagcgcg
    18601 tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc gagttgccga
    18661 tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc gattccggat
    18721 gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg acaaattccg
    18781 cagtgatatt ttccggcaga cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa
    18841 cttcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat tccatttccg
    18901 gtgtgatgat gccctggcgg gcgtagtgca gttgtgtgac gcggcgtcct gctttggcgc
    18961 gttttggcgt taatacgccg ctaaaacgca gctcgtcgag gccatcatct gccagccgcg
    19021 ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc
    19081 acggctggcg tagttttgcc agcccttgct gcacgttaat ggcgatctgc ggatcgccat
    19141 acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc ggctgttctt
    19201 tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg cgcacgccgg
    19261 gttgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct tccagggtgt
    19321 cgataaaatg ttgggcctgg gcgcgttgtt cgcgacgggt cagttttgtt gcagacatag
    19381 ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa gagacgatcg
    19441 ccgcaaaggc gatgcgatag cagattaact cttgttccct tcgcaggtat tagcctgatc
    19501 aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga caagaaatta
    19561 gccccacaaa cggggcgttg aatgtaaatt acgcgttaac agcgcagaac tcaagcagga
    19621 tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag cacaaagcgc
    19681 gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta attatcatga
    19741 tcgatagcgg tttccaggct ggaatcgtag taatccataa tctgttgggt attggcttcg
    19801 agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga
    19861 atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct ctgacaaaaa
    19921 tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt aatgccaacc
    19981 agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc aaccagtttg
    20041 ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat tgactccgca
    20101 agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac atgccagtga
    20161 tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca cggctggtgg
    20221 gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata tggcgaagcg
    20281 gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca gggggaacct
    20341 gtttggttaa tacaacagca gcggcgttac gatatggggt cggtacgtca ggtcattgat
    20401 ctcgatgttg ggctgtttca actggccgga cgtggcgtac aactggcgga gttttaccga
    20461 tcgcataaat actgtggtta ctgcgggcat gaaatgtatc caagcaaaac cgaatgggcg
    20521 atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg cattattgtt
    20581 gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg taacggtgtc
    20641 catacggtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg
    20701 gaagtgatgg aagagagcgg aattaaagtt aaacacttgc gttatgtgac ttcccagccg
    20761 tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag cggcgacatc
    20821 gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga tttgccgtta
    20881 ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg
    20941 gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc caaaatgacc
    21001 gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga tgtcactcct
    21061 gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac gcgcgcccag
    21121 gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgtgaagt gaccttgcaa
    21181 ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct caccgtcccg
    21241 gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt tacctcgcca
    21301 gtcacctgca aagctgacgt cgataaactg ccaattccgg acccggaaga tgaactgggg
    21361 tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt gccgctgatt
    21421 ggtttttccg gcagcccgtg gacgctggcg acctacatgg tggaaggcgg cagcagcaaa
    21481 gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca cgctctactc
    21541 gataaactgg cgaaaagcgt cgctttgtat ctgaatgcgc agattaaagc cggtgctcag
    21601 gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc
    21661 tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga cggtcgccgc
    21721 gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat ggcagaaacc
    21781 ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg
    21841 ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc
    21901 cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga aggtcatgtc
    21961 tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg
    22021 gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagcgattat ggatctcgcg
    22081 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga tcgactcgat
    22141 aaggatccgc cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    22201 acgcgagcgg cgatggtgct gctgaaatac ccctcgcttg agctggtgga gtataaagtg
    22261 gcccgcatcg ctaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    22321 gcgctgctgg cagcgtggga gatgctgtca caaaagccgg atttagtgtt tgtcgatggt
    22381 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggttt gctggtggat
    22441 gtgccgacca ttggcgtagc gaaaaaacga ctatgcggta aattcgaacc gctctccagc
    22501 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    22561 agcaaagcgc gctgtaaccc gctatttatc gctactggtc atcgggtcag cgtggacagc
    22621 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    22681 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22741 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22801 catctgcgtc tggaacgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22861 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22921 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga tgcaaaagta
    22981 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    23041 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    23101 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    23161 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gctcaaagaa
    23221 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    23281 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgaggcggat
    23341 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    23401 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    23461 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    23521 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    23581 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    23641 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    23701 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23761 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23821 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23881 ttgtctttct ggcgtcgatc attgatgctg gctggcgctc tgtttctcac tgcttgtagt
    23941 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    24001 cgcatctggc gaaaagacag cggcgataat gtgcattttc ttgccgtgtt tagcccgtgg
    24061 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    24121 aatatcaatg tttacagcaa acccccggtg aatattcgcg cgcgttttga cgatcgcggt
    24181 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    24241 atcgatttat accgttatcg ggctgatcag atccgccaga ttagcgatgc cttacgtcag
    24301 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    24361 gaagggcaaa ccattaaacc tgatttagat tcgcaggcaa tagcgcatat cgagcgccgc
    24421 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    24481 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    24541 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    24601 ttccggctcc gcgcggaata cccgcttcag ccatcgcaat atcccgtttc acccgtaact
    24661 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24721 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24781 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24841 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24901 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24961 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    25021 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    25081 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    25141 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    25201 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    25261 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    25321 ccgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgtgc
    25381 tctcgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    25441 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    25501 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attccccgca
    25561 ggaaatgcag cagttacatc cgggagatga agaagtgtgg cggcggatcg atagcgcaga
    25621 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    25681 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25741 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttatcctctt
    25801 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25861 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25921 gcatcttgcg gcaggcgttg cccacgaaat tcgtaacccg ctttcctcga ttaaagggct
    25981 ggcaaaatac tttgccgagc gcgcgccagc agggggagaa gcgcatcaac tggcgcaggt
    26041 gatggcgaaa gaagctgacc gtttaaaccg cgtggtaagc gagctgctgg aactggttaa
    26101 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    26161 gctggtaagc caggatgcaa actgccggga gatccagtta cgctttaccg ccaacgacac
    26221 gttgccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctttatct
    26281 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    26341 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    26401 agccatcttc accccgtact tcaccaccaa agccgaaggc accggactgg ggctggcggt
    26461 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc tggagggaaa
    26521 aggcgcaagg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    26581 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    26641 caggctttgc tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    26701 ctggagcagg tgcgggagcg ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26761 atggacggca ttgccacgct aaaagagatc aaagcgttaa atccggcgat tccggtactg
    26821 attatgaccg catactctag cgtcgagacg gcggtagagg cgttgaaaac cggggcgctg
    26881 gattatctca tcaagccgct ggatttcgat aacctgcagg cgacgctgga aaaggcgctc
    26941 gcgcatacgc acattattga tgctgaaacg cctgcggtga ctgccagcca gttcggtatg
    27001 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    27061 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    27121 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    27181 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    27241 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgttgtt tctcgatgaa
    27301 attggcgata tctcgccgat gatgctggtg cgtctgctgc gtgcgattca ggagcgcgaa
    27361 gttcagcgtg tcggtagcaa ccagactatt tcggttgatg tccggctgat tgccgcgacc
    27421 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    27481 ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tattcctctg
    27541 ctggctggcc attttctgca gcgctttgct gaacgtaatc gcaaggcggt aaaaggcttt
    27601 acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat tcgtgaactg
    27661 gaaaatgcgg tggaacgggc ggtggtgctg ctgactgggg aatatatttc cgaacgcgag
    27721 ctgccgctgg cgattgccag tacgccgatc ccgctggcac aaagcctgga tattcagccg
    27781 ctggtggaag tcgaaaaaga agtgattctg gcggcactgg agaaaacggg cggcaacaaa
    27841 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tgttggcaaa attgtcgcgt
    27901 tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc
    27961 cagtggatat cggtcattaa ggcataggcg cgtttctgtg cttctgctac ggtatgaccc
    28021 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    28081 tttgtgcccg cgtggaacac tttgccgtct tccacttctt ccagcggcag gccgtggatc
    28141 acgtcaccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gccgagagaa
    28201 gcacgttcat cccactctga cgttttctcg tctagtttgc cttcacaggc cgccaggcag
    28261 agttcaacaa gatcggactt catgcgcagc ataatcggct gggtttctgg atcgccaaag
    28321 cggcagttaa attcgataac cttcggattg ccctgtttgt cgatcatcag gcccgcgtag
    28381 agaaaaccgg tgtaggtgtt gccttccgac gccatgcctt tcacggttgg ccagatgata
    28441 cgttccatcg tgcgctgatg aacgtcatct gtcactaccg gcgcaggaga gtaagcgccc
    28501 attccgcccg tgttcggccc ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg
    28561 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccgtcg
    28621 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa acgcgttgcc cgccagcata
    28681 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28741 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata cgccagcgca
    28801 ggttctacct cggtgaagtt ctgatattcc gccgtaggaa tgttatggcg ggccaggaaa
    28861 tctttagtaa acgctttcga gccttccagc tgtgcggcgc ctgcggttgg gccgaagatt
    28921 ttcatcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28981 acgatggtta gatctacctt ttcgttttgc gcgaaatcca gcagcgccgg gatatcggtc
    29041 acgccgatag cgacgttttg cagcgtgggt tccagcgctg tgcctgcgtt acccggagca
    29101 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    29161 ccgccgttac caatcactaa tactttcatc tgtcgctcca ttaatggcgg aagtggcgca
    29221 tatcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    29281 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    29341 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    29401 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    29461 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaacttcg
    29521 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgtttggtga
    29581 cgacgcgcag ttcttccgca ccgaccatgc ccagatcgcg atcctgaacc agcagaccgc
    29641 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    29701 cgcgtacatt ctgtttggcg gcggtgattt tcagggcttc ttcgctggcg gacggcgcaa
    29761 taatcacttc aacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    29821 ggttaaaggc aatgatgccg ccgaatgcgg aggtcgggtc ggttttgtac gcgcgatcgt
    29881 aagcatcaag aattgaattg ccgatagcca cgccgcaagg gttggcgtgc ttcacaatca
    29941 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    30001 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    30061 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    30121 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    30181 caccgtgata agccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    30241 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    30301 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    30361 catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata tcgatgttct
    30421 caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac ggatacaggt
    30481 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    30541 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    30601 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    30661 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    30721 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30781 gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc tgaatttttc
    30841 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30901 aaaacgtttg cgcaacgctc gcgaattttt ttaattcgat ggcgatcacg attttgcccc
    30961 cgattagcgt gggagaaata gagaaataac gctgatgatt gtggataact ctgtgcgtaa
    31021 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    31081 tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg cggatgtgaa
    31141 tcacttcaca caaacagccg gttcggttga agagaaaaat
//
LOCUS       DAZFKY010000040        29836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFKY010000040 DAZFKY010000000
VERSION     DAZFKY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            65..1663
                     /gene="rtcR"
                     /locus_tag="ACHKCL_001595"
     CDS             65..1663
                     /gene="rtcR"
                     /locus_tag="ACHKCL_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO5762592.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1645..2403)
                     /locus_tag="ACHKCL_001596"
     CDS             complement(1645..2403)
                     /locus_tag="ACHKCL_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5762593.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(2420..3250)
                     /gene="glpG"
                     /locus_tag="ACHKCL_001597"
     CDS             complement(2420..3250)
                     /gene="glpG"
                     /locus_tag="ACHKCL_001597"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5762594.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3295..3621)
                     /gene="glpE"
                     /locus_tag="ACHKCL_001598"
     CDS             complement(3295..3621)
                     /gene="glpE"
                     /locus_tag="ACHKCL_001598"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5762595.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3811..5316
                     /gene="glpD"
                     /locus_tag="ACHKCL_001599"
     CDS             3811..5316
                     /gene="glpD"
                     /locus_tag="ACHKCL_001599"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5762596.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5531..6133)
                     /locus_tag="ACHKCL_001600"
     CDS             complement(5531..6133)
                     /locus_tag="ACHKCL_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762597.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6133..7125)
                     /locus_tag="ACHKCL_001601"
     CDS             complement(6133..7125)
                     /locus_tag="ACHKCL_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5762598.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(7151..8659)
                     /locus_tag="ACHKCL_001602"
     CDS             complement(7151..8659)
                     /locus_tag="ACHKCL_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5762599.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(8784..11231)
                     /gene="glgP"
                     /locus_tag="ACHKCL_001603"
     CDS             complement(8784..11231)
                     /gene="glgP"
                     /locus_tag="ACHKCL_001603"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5762600.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11250..12683)
                     /gene="glgA"
                     /locus_tag="ACHKCL_001604"
     CDS             complement(11250..12683)
                     /gene="glgA"
                     /locus_tag="ACHKCL_001604"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5762601.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12683..13978)
                     /gene="glgC"
                     /locus_tag="ACHKCL_001605"
     CDS             complement(12683..13978)
                     /gene="glgC"
                     /locus_tag="ACHKCL_001605"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5762602.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(13996..15969)
                     /gene="glgX"
                     /locus_tag="ACHKCL_001606"
     CDS             complement(13996..15969)
                     /gene="glgX"
                     /locus_tag="ACHKCL_001606"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5762603.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(15966..18152)
                     /gene="glgB"
                     /locus_tag="ACHKCL_001607"
     CDS             complement(15966..18152)
                     /gene="glgB"
                     /locus_tag="ACHKCL_001607"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5762604.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18425..19528)
                     /gene="asd"
                     /locus_tag="ACHKCL_001608"
     CDS             complement(18425..19528)
                     /gene="asd"
                     /locus_tag="ACHKCL_001608"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5762605.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19720..20313
                     /gene="yhgN"
                     /locus_tag="ACHKCL_001609"
     CDS             19720..20313
                     /gene="yhgN"
                     /locus_tag="ACHKCL_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5762606.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20359..21654)
                     /locus_tag="ACHKCL_001610"
     CDS             complement(20359..21654)
                     /locus_tag="ACHKCL_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO5762607.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21561..23685)
                     /locus_tag="ACHKCL_001611"
                     /pseudo
     CDS             complement(21561..23685)
                     /locus_tag="ACHKCL_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(23825..25165)
                     /gene="gntU"
                     /locus_tag="ACHKCL_001612"
     CDS             complement(23825..25165)
                     /gene="gntU"
                     /locus_tag="ACHKCL_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5762608.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25169..25696)
                     /gene="gntK"
                     /locus_tag="ACHKCL_001613"
     CDS             complement(25169..25696)
                     /gene="gntK"
                     /locus_tag="ACHKCL_001613"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5762609.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25835..26830)
                     /gene="gntR"
                     /locus_tag="ACHKCL_001614"
     CDS             complement(25835..26830)
                     /gene="gntR"
                     /locus_tag="ACHKCL_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5762610.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27054..27749)
                     /gene="yhhW"
                     /locus_tag="ACHKCL_001615"
     CDS             complement(27054..27749)
                     /gene="yhhW"
                     /locus_tag="ACHKCL_001615"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5762611.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27872..28909)
                     /locus_tag="ACHKCL_001616"
     CDS             complement(27872..28909)
                     /locus_tag="ACHKCL_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5762612.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29243..29731
                     /gene="yhhY"
                     /locus_tag="ACHKCL_001617"
     CDS             29243..29731
                     /gene="yhhY"
                     /locus_tag="ACHKCL_001617"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5762613.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT         7427 a         8045 c         7322 g         7042 t
ORIGIN      
        1 cggagatttc ccgcaaagcc aatttaccga taatgaaata tcgtctttta taaggatatc
       61 taagatgcgt aaaacagtgg cttttggctt tgtcggaaca gtgctggatt atgccgggcg
      121 cggcagtcag cgctggtcga aatggcgtcc gtcactctgt atatgccagc aagaatcgtt
      181 agtcatcgat cgactggaat tattgcatga cgcccgctcg cgctcgctat ttgaaacgct
      241 taaacgcgat atcgccagcg tttcgccaga aaccgaagtg gtgagcgttg agattgaact
      301 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
      361 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
      421 gcagatttgc tggtttctgc tggctgaagc gcgttacctg cccgcccggt tgatacaatc
      481 ttcacctccg cgcaaaaaag aacagccgcg cggcgcaggt gaagtgacga ttatcgatct
      541 cgatttaagc ctttataacg ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct
      601 tgattttctt aagtccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
      661 gattgaaaaa gtagcgatta aatcccgcgc accgatcctg ctaaacggcc caaccggtgc
      721 gggcaagtca tttctggcgc gacgtatatt tgaattaaaa caggcgcggc atcagtttag
      781 cggcgcgttt gtggaagtga actgcgcaac cctgcgcggc gataccgcca tgtcgacgct
      841 gtttggtcat gtgaaaggcg cgtttaccgg ggcacgggaa tctcgtgaag gattattacg
      901 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
      961 ggcaatgctg ctgaaagcca ttgaagaaaa aaccttttat ccgtttggca gcgatcgcca
     1021 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgt gatttgcgcc agctggttgc
     1081 cgatggcaaa ttccgcgaag atctatacgc gcggatcaat ctctggacct tcaccctgcc
     1141 ggggctacgc cagcgtcagg aagatattga acctaacctg gattatgaag tggagcgcca
     1201 cgcttcactc accggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggctggc
     1261 ctttgcgaca tctccccagg caacatggcg cggtaacttt cgcgagcttt ctgccagcgt
     1321 cacgcgaatg gcaacctttg cctctagcgg acgcattact ctggacgtgg ttgaagatga
     1381 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
     1441 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
     1501 aatctgccgc caggcaaagt cgctttccgc agccggacgc cagctttttg acgtttcgcg
     1561 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
     1621 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
     1681 cacctgcatc acgcttactg gcggcggggt gtcggtgtag acggcatcta ccatgctgat
     1741 gctacccata ttgaccatcg cgttacggcc aaatttcgag tgatcgacaa ccagcataac
     1801 gtggcgcgag ttctcaataa tggcgcgttt ggtgcgaact tcgtggtaat cgaactccag
     1861 cagcgagccg tcgctatcga tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg
     1921 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc
     1981 accggcgaga ataatacgga aatcctcttt taccatcagc gtgttagcaa cgttaagatt
     2041 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
     2101 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
     2161 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
     2221 actggaaggc agcgccgcac cgccatgatg gcgcaggatc aggttttgct ccgccagctc
     2281 attgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gctcctcggt
     2341 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
     2401 catttctaaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
     2461 tcgctaaacc cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
     2521 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
     2581 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
     2641 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
     2701 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
     2761 caccgccgag ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca
     2821 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgctggatcg aatggccaag
     2881 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
     2941 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggcg gcaaagaaag
     3001 gataacggcg ataatgcagg ccactgccgg tatggcctgc ctgccagctc gccgccagat
     3061 aacgcggatc tgccgggttt tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct
     3121 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
     3181 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
     3241 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
     3301 gccgtacgcc acctctgcgg gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct
     3361 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc
     3421 gtgataacac atcaccatca ccggagtgtc aaagtcgtta ttacgcataa aagcgcccag
     3481 cgtgtcgttg gttaaatgga aagcctgcac cgcatgtccc attgcgaaac tctgtggatc
     3541 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
     3601 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
     3661 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
     3721 gttttttcaa tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta
     3781 tgaactttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
     3841 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
     3901 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
     3961 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
     4021 cgtgaagtgc tgctgaaaat ggccccgcat atcgccttcc cgatgcgttt tcgcctgcca
     4081 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
     4141 ctgggtaaac gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg
     4201 ttaaaaccgg aaattaagcg cggattcgaa tattccgact gttgggtaga cgatgcccgt
     4261 ctggtgctcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcgtact
     4321 cgtgctactt ctgctcgtcg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
     4381 accggcaaaa aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg
     4441 aaacagttct tcgacgacgg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
     4501 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
     4561 gaagataaac gtattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
     4621 accgatgtgg aatacaaagg cgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
     4681 tacctgctga aagtgtataa cgcgcacttt aaaaaacagc ttagccgtga cgatatcgtc
     4741 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt
     4801 acccgtgatt acacccttga tattcatgat gaaaatggca aagcaccgct gctgtcggta
     4861 ttcggcggta agctgaccac ctaccgaaaa ctggcggaac atgcgctgga aaaactaacg
     4921 ccgtattatc agggtattgg cccggcatgg acgaaagaga gcgtgctacc gggtggcgcc
     4981 atcgagggcg accgcgacga ttatgccgct cgcttacgcc gccgttatcc gttcctgacc
     5041 gaatcgctgg cacgtcatta cgcgcgtact tatggcagca acagcgagct gctgctcggc
     5101 aatgcgggag cggtaagcga tcttggggaa gatttcggtc atgagttcta cgaaacggag
     5161 ctgaaatatc tggtcgacca cgagtgggtc cgccgcgccg acgatgccct gtggcgtcgc
     5221 acaaaacaag gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg
     5281 gaatatacgc agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag
     5341 atttcaatct ggcctgagac tgatgacaaa cacaaaattg cctgatgcgc tacgcttatc
     5401 aggcctacgt ggtttatgca atatgttgaa tttgcatgat tttgtaggcc tgataaggcg
     5461 ttcacgccgc atcaggcatg aacaaagcgc cacgttgtca acaatctgac ctactttttg
     5521 attcagggaa ttaccgcaaa gcccacgcca tcatacccaa cgtaacaaaa cttaagcacg
     5581 ttccccactc actccacttc cgtatctggc ttctattttt gctacgccac tggcaataat
     5641 atcccaacgc aaaaccaagc tgtgcactta tcggaccgac aaagtcgatc cactgttgag
     5701 accaaaatgg cgaaaaattc agtgggagcc agacaactgt cagaaagaaa ctgcgcatat
     5761 tttccagcaa agcaatttgc gctggcgcga cataaaaaat gatattccac aggagcaacc
     5821 agaaataaac tggtgcaaat accagccaac aatgcagtct gcttttcaat tcaggtggcg
     5881 ttctgcgcgc aaattgccaa ccaacgccca ccattgcaca gaagaaaagc gcatattcag
     5941 ccgataacca tagttgtaat tgcagaaggg aaatacgcct gtgcatgagc gggattgaga
     6001 acagtatttc tcccagaaaa ctcagcgtga ttacacaggc agccagcgca accaccgtcg
     6061 gccacaccaa caacggtaca ataacaaagc cgagaaataa cggataaata taaagtctga
     6121 ttatattcat cattattgtc ctgatgattc gggggatgct tcatacagcg gcacataccc
     6181 cacatcctct accaactgtt gcccctgctg acttataaac cagtcaacaa atttctgcgt
     6241 ttccggcgtg ggattttcac gcgtcaccat ataggcatcc acaatgtatg gataagtgcc
     6301 gttgcggata ttttcctcgg taggcgcaac gccatttata gccaaaagtt taatctgctt
     6361 gcgcattttg agcatattgt cattcatacg agtgacgtaa tagtggaagg tgtacccaat
     6421 ggaagatggc gtgttttgat agtcggcaac ttcagtaatt aaaccgccca taagatcaat
     6481 cacagtggat tttttagctg gcagcattgg ggtgtcttgc ataaccaacc ccttcataat
     6541 cgtttggctt ccagaatctt cagggcgctg ccagactttt atatgttcgt cactaccacc
     6601 tactttattc cagcggctag ttttgccgct aaaaatatct ttaacctggt gctcagagag
     6661 agaatttacc gggttattaa tatcaacgat aaagacaaag gcttcgcggg cgaaagcggt
     6721 atatttcagt ttaacacccg cttcttctgc acgttttttc tgtccttccg acggttgtgc
     6781 aacaaaaata accgtcgcgg aacctttaat cagactatcg taggcttcag ctgttcgggt
     6841 ttgctgcaaa ccatacgcct gccaggccag cgaggttcga tccttatctt ttggctcggg
     6901 tactaattta taatacgcgg aggcatacag cgggtataag gctgtagcgc cgtacactct
     6961 cggccatctt gaccaatcat cgctgtaacg taatgcaggt tgcccccgca gagacgtcag
     7021 tccatggctc tcttcttccc tgacgctgtt atagtaccct tcccggtcga cacatcccgc
     7081 cagtaacgcc gtgcctaata gtaaaacacc ggtgaattta ttcatctcgc tgattccttt
     7141 ctctcgagcg ttattccatt gttagataca gcggcacata tcccacatct tctaccagac
     7201 tctgcccctg cggcgttaaa aaccattcga ccagtttttg tgtttctgac gttgtatttt
     7261 ctctcgtcac cataaatgca tcgacgatgt acgcatattt gccgttgcga atgttttccg
     7321 ccgtcggtgt aataccgtta atcgctagca atcttatatt tttatcagca ttcatttgcg
     7381 tcgcgtaata gcggaaggta tagcctattg cgttgtttgt attacggtat tcggcaacga
     7441 ctttaatcat tccctccatc acgcttgcca cttccgtttc ctgcggcgag atcatgcgga
     7501 cttttttcat gacctgtgat tgcatcactg tctggctgcc agagtcttcc gggcgctgcc
     7561 aggtctggat ctcctgatcg ttaccgccaa cagtacgcca attggtaatt gcaccactga
     7621 aaatgtcacg cacctgttgt tcagtcaggg aattaaccgg attatccgca ttgacgatga
     7681 aaacaaatgc ttcacgggca aatggcgtgt atagcaaagt gacgcccgat tcctcagcgc
     7741 gttttttctg cccgccggag ggttgcgcca caaaaataat atcggcatca cctttaacaa
     7801 tccggttata tgcatcgggg gtacgagagt tctccagata ttcccaaacg tgaaaatcct
     7861 ctggtattac acttaatgca taaaatgcag aagcataaat tgggtacgca gccgtggcgc
     7921 catcgattcg cggccagttt tgcctaaact gaatttgcgg tttgccacgc agcggcgtca
     7981 gtcgattatt gagtttatcg ggtcgccagg ccctgatatc aatattttcg cgtacaccga
     8041 caccaggatt ttgctgctta tataaatggg cctgataccc ggcgacaata ccaagtaaga
     8101 gaagtagaaa caggagtaag ttacggcaac gtagtccgga cgtgccggat aaaggatatc
     8161 tggtcggcca ggcgtatccc agcgaaaaac tgatatgcgc cccaacggga ataatgacca
     8221 tccaccagta ctcaccaccc actaatgaga ctaaaaaaac gcccaaccag ggaagcataa
     8281 ggtttgccaa ccaaccagct cccatctggc gaaattgata atctgccaga tgtagcgtga
     8341 ttatccagcc aaccagggtt aatagcgcgg gtaagagaaa aaagcattgc cgccagacgg
     8401 ctatttctct gttttctggc gcgctatcac cggcccacac gcgaccagag aaaaatatta
     8461 ttatcgcccc cagcgtcagc ataaagacgt tgagtagaag ataaattgtt ggcgtgataa
     8521 ggatattaca aacttcaata ataccggtaa gcccgacacc cagcatggca acaaccgctg
     8581 ccaaaaattg cgccagtatg gggatgccga aaaaagtcat tatcagcgag gtcaaaatcc
     8641 attttctatt tggcatcatt ctgtccattc ttcctgaatg acggaattat actccccgag
     8701 aaccctcgcc ccctctggac acttttccga aataaaggcg aaaaaaacgg gaccttgtgg
     8761 ccccgttctg ttttttagcg aacttacaat ctcaccggat cgatatgcca gatatgatca
     8821 gcgtactctt tgatagtacg gtcagaagag aagtagccca tattggcaat gttcagcatc
     8881 gcttttgcgg tccactcttc ctgaagctcg tagagttcat cgactttatc ctgacaatcg
     8941 acatagctgc gataatccgc cagtacctgg tagtgatcgc cgaagttgat cagcgaatca
     9001 accagatcgc gatagcgacc cggatcttcc ggactgaata caccgctgcc gatttgcgtc
     9061 agcacctgat gcagctcctc atctttctcg tagtattcac gcggtttgta gccctgacga
     9121 cgcagttctt ccacttcttc cgctgtgtta ccaaaaataa agatattgtc agcaccgaca
     9181 tgatccagca tctcgacatt cgcaccgtcc aacgtaccga tagtcagcgc accgttaagc
     9241 gcaaacttca tgttactagt gccggaagct tccgtccctg ccagcgaaat ctgttcagac
     9301 agatctgccg ccggaatgat caactgcgcc aggctaacgc tgtagttcgg gatgaacacg
     9361 actttcagct tatcgccaat ctgcggatcg ttgttgatca ctttcgctac gtcattgatc
     9421 aaatggataa tatgcttcgc catgtaatag gccgaagccg ccttaccgcc aaaaatattc
     9481 acgcgcggta cccacttcgc atccaggtcg gccttgatgc ggttatagcg ggtaatcaca
     9541 tgcaacacat tcatcaattg acgtttgtat tcgtgaatac gtttgatttg cacatcgaac
     9601 aacgcctttg gattcaccac cacattcagc tgctgggcga tatactccgc cagacgcttt
     9661 ttattctcca gcttcgcctg atgcacagcg tgattaacca ttgggaaatc acagtgttgt
     9721 tgcagctcat taagcaggct aaggtcggtg cgccagttac ggcccaggtg ttcgtccagc
     9781 acggctgaaa gcgatgggtt cgctaccgcc agccagcgac gcggcgtcac accgttggtg
     9841 acgttggtga aacgacccgg gaagattttc gcaaagtcgg caaacaacga ttgcaccatc
     9901 agattagagt gcagttccga tacaccgtta actttgtggc tcacaacaac cgccagccag
     9961 gccatacgca cacgacgacc gttggattca tcaatgatcg acgcccgtcc cagcagatcg
    10021 gtatcgttcg gatactgttc ctgcaaggtt ttcaggaaat agtcgttgat ttcaaagatg
    10081 atctgcaggt gacgcggcag aattttaccc agcatatcaa ccggccaggt ttccagcgcc
    10141 tcgctcatca gcgtgtggtt agtgtaggag aagacctgac aacacacctc aaacgcgtcg
    10201 tcccagctaa attggtgctc atcgatcagc agacgcatca tctcaggaat cgacagtacc
    10261 ggatgggtat cattgagatg aatcgcgatt ttatccgcca ggttatcgta ggttttatgc
    10321 aactgataat ggcggcttaa aatgtcctga atggtcgagg aaaccaggaa gtattcctga
    10381 cgcaggcgca gctcacgccc ggagtaggtg gagtcatccg gatacagtac gcgagatacg
    10441 ttctcggagt ggtttttatc ttccactgcc gcgaagtagt caccctggtt gaatttaccg
    10501 aggttaattt cgctactggc ttgcgcactc cacaaacgca gcgtgttggt cgcgtcggtg
    10561 tcgtaaccag ggattatctg atcgtaagcg actcccagaa tctcttcggt ttcaatccag
    10621 cgcgtttttt taccttcctg ctgaatgcga ccaccaaaac ggactttata gcgcgtgttg
    10681 tggcgtttga attcccacgg gttaccatat tccagccagt agtcaggcga ctctttctgg
    10741 ctaccgttaa cgatgttctg cttgaacata ccgtagtcat agcggatgcc gtaaccgcgc
    10801 cccggcaacc ctaacgtcgc cagagaatcg aggaagcaag ccgccagacg tcccaggcca
    10861 ccgttaccga ggcccgggtc attttcttca tcaatcagct cttcgagatt taaccccatc
    10921 gcttccagtg cgccctgtac atcttcgtaa attcctagcg acaacatggc gttggagagc
    10981 gtacggccaa tcaaaaactc catcgacagg tagtaaacct gacgagtttc ttgcgacaac
    11041 tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat cgcgcacagc aaataacgtt
    11101 gcgttcagcc attcatgttt attggcgacg accgggtcct ttccaatcgt aaacatcagc
    11161 ttgtaagcga tagagtgctt aagagcttct acgctaagcg tgggcgatga atatgtaaac
    11221 ggagcattca tataggcgtt tcctgaaaac tatttcaagc gatagtaaag ctcacggtac
    11281 gacttcgccg cgacctgcca gctaaaatcc attgccatag cctgacgttg cacaaaccgc
    11341 cacagagaag gacgggacca cagtacaaaa gcacgtcgaa tagcccgtaa cagcgaccag
    11401 gcattactat cttcaaagac aaacccactg gcgacgccat ctgcaaggtt ctcaagagaa
    11461 cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca ctaacggcag cgtaccgtac
    11521 ttcaatccat aaagttgcgt taagccgcac ggttcaaaac ggctgggcac cagaatgacg
    11581 tccgcaccgc ccataatgcg atgcgaaaat gcttcgtgat agccaatctg aacgcccacc
    11641 tggccggggt attccgctgc cgccgcaagg aaaccttcct gcagcaccgg atcgcccgcg
    11701 ccgagtagcg ccagctgccc gccctgctcc agaagacccg gtaaggcctc cagcaccagg
    11761 tcgaggcctt tctggctggt cagacggctc accactgcaa aaagcggcac tttatcgtca
    11821 accttaagcc ccattgcgat ttgtaactgg cgcttatttt ccgctttatc ttccaacgta
    11881 tcgcgggtgt aacgcgaggc caatagtaag tccgtctctg gactccagat tttctcgtcc
    11941 acgccgttca gtacgccgga aagacgccct tcacgatgac gctgttgcaa caaaccttcc
    12001 ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt aggttggact gaccgccgta
    12061 atgtgatcgg catagtacag accggccttc aggaaagaga tttgtccgtt gaattccagc
    12121 ccatgaatat taaagaatga ccatggcaat tggatgtcat tcatgtgatg tgcataaaac
    12181 atgccttgat aggccaggtt gtgcacagta aacaccgact tcgccggacg cccgcgcgcc
    12241 gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg catgcaccac atcaggacgc
    12301 cagaatgggt caagcccgct ggccatttct gccccaaccc accccagcag cgcaaaacgc
    12361 aatacgttgt cggtataggc aaataagttg gtatcgtgat acgggcttcc cggacgatca
    12421 tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt tgtaatgacc gaacaacagc
    12481 gtgatatgcc cggcgaaggt atcacgacgg gatactacct gcgcatcggt cacgccacgg
    12541 cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa cgccgtctgc gatttgtgct
    12601 gcgggtaatg ccccaataac atcagccaga ccgccggttt taagcagcgg gaacatctct
    12661 gaacatacat gtaaaacctg cattatcgct cctgtttatg ccctaacttc cgtagcattt
    12721 cgcgcgttac cagcacgatg ccttcttctg aacgatagaa acgacgtgca tcttcctctg
    12781 cgttttcacc aatcaccatg ccttccggaa taacacaagc acgatcgatg acgcagcggc
    12841 gcagacggca cgagcgacct acccatactt ccggtaacaa tacggcggaa tcaatgttgc
    12901 agaatgaatt cacgcgaacg cgcgagaaca gaacggactg caccaccacc gaaccggaga
    12961 tcacacaacc gccggaaacc agtgagttaa gggtcatccc gtggctaccg gagcgatcct
    13021 gcacgaattt cgctggcggt aatgattcat tgtaggtgcg aattggccaa ttgcgatcgt
    13081 acatatccag ttccggcacc acagaggcca gatcgaggtt cgctttccag taagcttcca
    13141 gcgtacccac atcgcgccag tacggctcgg catccggatc ggattgtacg caagagagcg
    13201 ggaacgggtg cgcataggcc agaccggctt cggtgatctt gggaatcaaa tctttgccaa
    13261 agtcgtggct ggagttctca tcgcgatcgt cttcttccag cagttcatac agatagtcgg
    13321 cgtcaaagac gtagataccc atactcgcca gagatttgct cggatcgttc ggcattgacg
    13381 gcgggttagc aggtttttca acgaattcga taattttatc gttctcatca accgccataa
    13441 cgccaaatgc ggaggcttct tcaatcggta ctggcataca agcaacggtg caacgtgcgc
    13501 ctttttcgac gtgatcgata agcatacgcg agtagtcttg cttgtagata tggtcgcccg
    13561 ccaggatcac cacgtattcc gctttataac ggcgaataat atcgaggttt tgggtgaccg
    13621 catctgcggt gccgcgatac cagttttccc ctttcattct ctgctgtgct ggcagcagat
    13681 cgacaaactc gttcatttct tcattgaaga atgaccagcc gcgctgaatg tgctgcacca
    13741 gagtgtggga ctggtactgg gtgatcacgc ccatacgacg gatcccggag ttgatgcagt
    13801 tagacagcgc aaagtcgata atgcggaact taccgccgaa gtgtacggcc ggttttgctc
    13861 gcttattggt taaatccttc aggcgggtac cacgtcctcc cgccagtatc agggcaacag
    13921 atttcaatgg cagctggcgc gccaacatta agtgatcgtt cttctctaaa ctaaccatga
    13981 ctaactcctt ttttatcatc tctggaacac acacaatccg tgtgcaggtc cctgccagac
    14041 agccgtaatc actgggttat cctctccagc gaatggggga atggcgtgcc actccccagc
    14101 aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt gcgatcaaaa agcgatccga
    14161 gagcagaatt tgcagctgtt tcggcccgtt ttgccactca tccgtgctta aaggttgagc
    14221 atatcgattt agccaacgga cattgccgtc gccttcttcc caccagcgat tctccaccaa
    14281 agcgggaata cgcttgcgca gatggattaa cgcggcggta aatgcggtta aaccactgct
    14341 tgcctgcgac cagtccaacc aggttaattg gttatcctga cagtaggcat tgttattgcc
    14401 atgctggctg tgaccatgtt cgtcaccggc cagtaacatc ggcgtaccct gggagagcaa
    14461 caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt tcaaccaggt caagggtacc
    14521 gcctacccct tctttaccat gattgttact gtaattgttg ttggtcccgt cgcgattttc
    14581 ttctccgttt gcttcattgt gtttatggtt gaaacaaacg cagtcgcgaa gcgtaaaacc
    14641 gtcatgcgcg gtgacgagat taatcgcggc actcggcaga cgaccattac gtttaaaaac
    14701 atcgctggag gcagcaaaac gcccggcaaa cgcccccaga ggcaaatcat aatgtagcca
    14761 gaaacgacgg gcagcatcgc ggaaatgatc gttccactcg gcaaacagcg gcgggaaatt
    14821 tcccacctga taaccaccag gagcgatatc ccacggttca gcaattaact tcacctgcga
    14881 gagcaccggg cagttctgga tagcggtaaa caacggcgca tcctgacgga actctggcgt
    14941 acggcccatg actgccgcca gatcaaagcg gaaaccatcg acgtggcagg tttctaccca
    15001 ataacgcagg caggcgctgg cataatccac caccgccggg tgactcaaat tgagcgtgtt
    15061 gccacaaccg gtccagttgt gataatcgcc gtcttctctt atccaataat agctacggtt
    15121 gtcgatccca cgcagcgaga ataacgggcc gtcgaggtcc agttccgcac tatggttgag
    15181 cacgatatca agaatgactt cgatacccgc tttatgcagt gctttgattg catcgcgaaa
    15241 ctcatgcagc gccgtttctg gcgagcaggc atacgccgga tgcagcgcaa acatcgccac
    15301 cgggttgtaa ccccagtagt tacttagccc catgcgttgc agacgtggtt cactggcaaa
    15361 ctgcgccact ggcagcagtt ccagcgcggt aatgcccaat tgtttcaaat agttgatcat
    15421 caccggatgc ccgagggctt tataagtgcc acggatctcg accgggatct ccgggtgcag
    15481 gtacgttaat cctttaacat gggcttcata aatgatggtg ctgccccacg gcgtgcgcgg
    15541 cggggcatca tcttcccagt catagtgatc aaccaccact acgcatttcg gcgcaatggc
    15601 ggcgttgtcg cgatagtcag gttcattatg accggcgtgc agcagcgggt tatctttaaa
    15661 ctccccgtca atttgccgcg cgcaaggatc aatcaacaac ttcgccgggt taaagcgatg
    15721 cccctcggcg ggttgccagg ggccatgaac gcgataacca taacgcaaac ccgggcgcgc
    15781 atccggcaga taaccgtgcc aaatgtcgcc actgtgccct ggcaagtcat agcgatgttc
    15841 ctggccattg gcgtcaaaga cacacagttc tacccgctcg gcatgagcgg agaaaagtgt
    15901 gaagttgacg ccctgaccgt cgtaatgcgc gccgagggga gcgggtttgc caatggcgag
    15961 ttgtgtcatt ctgcctcccg aaccagccag atagtggcca gcggtggtag cgtcaggctt
    16021 agtgaatgct gacgaccgtg gctggcaatc tcatcgctgt gtaccgtgcc gccattacct
    16081 gcattactgc cgtgatagtg catggaatcg gtattgagga tctcacgcca tttgcctggc
    16141 tgattaatgc cgaagcgata atcatgacgc ggcaccggcg taaagttact ggcaacgatg
    16201 atttcgttac cctctttatc gcgacgcaca aagatcagca ccgagcgttc tttgtcatcc
    16261 accaccagcc attcaaagcc gtacgggtca aaatccagtt catgcatcgc tttatggtgg
    16321 cggtaggtga ggttcagatc gcgcaccaga cgctggacac cgtggtgcca gttatcgccg
    16381 ccttccaaca gatgccagtc gaggctggcg tcatggttcc actcgcggcc ctgggcaaat
    16441 tcgttcccca tgaacagcag tttcttgccc ggaaatgccc acatccagcc atagtaagcg
    16501 cgcaggttag cgaatttctg ccatgcgtcg cccggcatgc ggtcgagaat cgatttttta
    16561 ccgtggacca cttcatcatg cgacaacggc aggacgaagt tttcagtgta gttgtagagc
    16621 atcccgaagg tcagtttatc gtgatgatac tgacgataaa tcgggtcgag cttcatgtag
    16681 tccagggtgt catgcatcca gccgaggttc cacttgtacc agaagcccag accgcccata
    16741 tcctgcggac gagaaacgcc agggaaatcg gtagactcct cagccattgt caccgcaccg
    16801 gaaacctgct caccaagaat acggttggta ttacgcaaga attcaatcgc ttcaagattc
    16861 tcgcgcccgc caaattcgtt cgggatccac tccccctctt tacggctgta gtcacgataa
    16921 atcatcgacg ccaccgcatc gacgcgcagc gcatcaatac caaaacgttc aatccagtaa
    16981 agcgcgttac cgacgaggaa gttactgact tcacggcgac catagttgta aatcagcgtg
    17041 ttccagtcct gatgatagcc ttcgcgcgga tcgctgtgtt catacaagtt cgtaccatca
    17101 aattcggcaa gcgcaaagtc atcagtcggg aagtggcctg gcacccagtc gagaatcacg
    17161 ttcagaccag ctgcgtgtgc agcatcaatg aaataacgga agtcgtcgcg agtaccaaaa
    17221 cggcgggttg gtgcatacag gccggttggc tgataacccc aactgccatc gaagggatgc
    17281 tcgttaaggg gcagtagttc gaggtgggta aagcccatcc atttagcata aggcaccagt
    17341 tgatcggcca gctcgcggta gctcaaccag aaattgttgt cggtgtggcg acgccaggag
    17401 cccaggtgaa cttcataaat agagattggc gcatcaaact gattcgcttt tttgcgctct
    17461 tcagtctgta caaccttttc cggcagcccg caaataagag acgcggtttc cgggcgcatt
    17521 tgcgcttcga aggcataagg gtcggacttc agacgcaagt tgccattggc atcaatcatc
    17581 tcgtatttat agagctgacc gttatgcgcc ccagggataa acagttccca gatgccgctc
    17641 tctttacgca ggcgcatcgg gtgacggcga ccgtcccagt agttgaattg cccaaccacc
    17701 gagacccgac gggcgtttgg agcccagaca gagaaacgcg tacctgtgac gccatccata
    17761 gtatctgcat gcgcgcctaa agtttcatac gggcgcaggt gagtaccttc agataatagc
    17821 caggcatcca tttcctggat tagcggacca aaacggtaag gatcatcaat caggttttgc
    17881 tgcccatgcc agacaacagc caactgatag cggaaaaaat tcttacgtcg cggaatgacg
    17941 ccactaaaga atccccgtga gtcgagacac tccagttttg cgagtttgcg cccggttttc
    18001 ggttcaatca cccacacatc ggtagcgtcg ggtaaaaggg cacggacttc cagtcccgcg
    18061 gtggttttat gcattcccag tacggaaaaa ggatccgcaa aatggcctgc aattagcgcg
    18121 ttaatcacgt ctctatcgat acgatcggac atgcttgtct tcctgtttta ttgtgtcacc
    18181 ccatccaatt ggatttttga cttctggttg tgacattttt ttgacctgaa cggcgcagca
    18241 ctctgtgcat cctctctgcg tcgtcctcac ttcaggtaag gctgtgaata ctcatgtatt
    18301 cagccaccct taaagaatag ccaatgctct atttaactcc cggtaaatca tgaaacatct
    18361 gcgcttactc ctgtattacg cactaacagg ggcggcatcg cgccccagat ttaatgaata
    18421 aagattacgc cagttgacga agcatccgac gcagcggctc cgcggccccc cacagcagct
    18481 ggtcgcccac ggtaaaggct gacaggaact ctggtcccat attcagctta cgcaggcggc
    18541 ctaccggcgt ggtcagcgtg ccggtaacgg cagctggggt tagctcacgc atagtgattt
    18601 cccgatcgtt cggaacgact ttcgcccacg gattgtgcgc agccagcagt tcttccacgg
    18661 tcggaatcga cacatctttt ttcaatttaa tagtgaatgc ctggctgtgg cagcgcaatg
    18721 ccccgacacg cacacataaa ccatctaccg gaattacgga agatgtgttg aggatcttgt
    18781 tggtttccgc ctgccctttc cactcttcgc ggctctgacc gttatcgagc tgtttgtcga
    18841 tccacggaat caggctaccc gccagcggca cgccaaagtt atccaccggc agctcaccgc
    18901 tacgggttaa ggttgtgact ttgcgttcga tatcgagaat agcagaggac ggggtcgcga
    18961 gttcatctgc tacatggcca tacagatggc ccatctgggt taataactca cgcatatgcc
    19021 gcgcaccacc gccggaagcg gcctggtagg ttgcaacgga cacccaatca acaagatcat
    19081 tggcgaataa accacccaac gacatcaaca tcaggcttac ggtacagtta ccgccaacaa
    19141 aagtcctgat gccattattt aatccgtcgg taatgacgtc ctgattgacg gggtcaagaa
    19201 tgatgatggc gtcatctttc atgcgcagag acgatgctgc gtcaatccag taaccctgcc
    19261 atccgctttc acgaagcttt ggatagattt cgttggtata atcgccgccc tgacaggtca
    19321 caatgatatc gagggccttt agcgcctcca gatcaaaggc atcctgaagt gtgccagtgg
    19381 ttccgccaaa agacggcgca gcctgaccaa gctgagaagt agaaaagaag acagggcgaa
    19441 tggcgtcgaa gtcgcgctct tcaaccatgc gttgcatgag aacggagccg accataccgc
    19501 gccagccgat aaaaccaaca tttttcataa gcgttttttc ctgcaaagat gtgtgctgta
    19561 taaatgtgcc ggtctcctct tggcacatct ttcaccatac aaaaagcagc caaagtcgca
    19621 agtgaaatta atcaatgata gcgaagccat cagtaatgcg acttatcctg ctttgttagc
    19681 acgcagaaag tccgcggcaa ttatcaggga atttgagtta tgaatgaaat catttctgca
    19741 gcagttttat tgatcctgat tatggatccg ctcggaaacc tacctatttt catgtccgta
    19801 ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt gcttattgct
    19861 ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt tctcagcctg
    19921 cgagcagaaa ccgtctccat ttctggcggc atcattctgt ttctaattgc cattaagatg
    19981 attttcccca gcgcttcagg aaatagcagc gggcttccgg caggtgaaga gccatttatc
    20041 gtgccgctgg caattccgct ggtcgccggg ccaactattc tcgccacgct gatgttgttg
    20101 tctcatcagt acccgaatca gatgggacat ctggtgattg ctctgctgct ggcctggggc
    20161 ggcacctttg tcatcctgct acagtcttcg ctatttttac gtctgctggg cgagaaaggg
    20221 gtgaacgcac ttgaacgcct gatgggattg attctggtga tgatggcaac ccagatgttc
    20281 ctcgacggca ttcgaatgtg gatgaagggg taaggttcaa acaggtgctg cacacaacca
    20341 gacgcggcac ctgttttatt acctgaactg ataatgacct tcgcccaatc ggataaccct
    20401 tccctcttct tctgcgcggt tcagccacac actaatttgt tcgatggtta aaccagtaca
    20461 agacgccagc ctttcaatac ttatcgattc ctttgcaagg atcgccagtt ccgccacaaa
    20521 gagttgataa aaatcgacag gcgtatacgc gggcattact gcgtaatttg cgtcatcttc
    20581 atgcagcgaa aacagttctg gttgagcgtt acggagtgct ctgtcataca tcagcccagg
    20641 aacgtctggc agtctgatcg tcacattctc agcctgttca gcactccatg acgccccatt
    20701 ttctactaat cgtgaattgg ctgattgcat atcctgattt ggtttaacct gcacaggcag
    20761 ccactgatgt tttaatgcct ccatcgcccc ggttatcgtt ccaccggtca ttcccgcacg
    20821 tacaaccatt gcgctttcag caaggcaata aatatattta tttcgcgcca tcgcattgcc
    20881 gacggtgaaa cgtacctctg ggtaaaacgg cgaaatcaac accaggttgc ctgctataag
    20941 cccttcacgc catttcacta acgtactcgt ttttaacaag ctatcagcta atacgccaac
    21001 ggcagttccc ccggcctcca gtgccgacgc cattgcacat tcatcaatac ctcgcgcacc
    21061 accagagata acgcaaatcc cctgttgggc cagtttcgcg gccagttgtt gggtatagcg
    21121 caaatcgtca gtcggagcat ctcgcgagcc aacaatcgcc ataccttccg cttgcagtaa
    21181 tgctttattc ccgcagccaa ataaaacggg aggtgcatcc gttcgcaatc ggtttttcaa
    21241 gcgaacggga taatcagcat ctccgcgggt taagatccat aaaccggcgc gttgccactt
    21301 atctaccgcc aacgccagac tatgaccacg cgccagcaag ccaagaatac gctcctgaga
    21361 aatacgcgga tcgctccagc ctgtaagttt ctcttgcggt tgcggcacca gaagctcggc
    21421 gggactgata cgttggtgct tcagccataa tgcaaacttc ccccattcac tattactaag
    21481 aggtttatac ttactcgccg ccgcgcgaga aaaatcgctg gttagcagga gagttgcttg
    21541 tgcattggct gaaagattca tcaatttttt accgaggtag aagcaagggc aagaggataa
    21601 accgtcgggc aacctgcctg gcgtaacagt gctgttagaa ctgtcagcgt ccatgcagaa
    21661 tccacgatat cgtcaaccag caacgccgga ccaggcatca aaggaggggt aatcacaaac
    21721 gccccgtcga gattttgaca ctggtggaaa cggttttgct gcattttctg cggtggattg
    21781 tccacgactt tttcaatggc atcaataaaa ggtaagccaa gtttcgccgc cagtcgccgg
    21841 gcaaaatcag gaaccaggtc gaggtgcttt caaaatcagg aaccaggtcg aggtgcttta
    21901 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
    21961 tttccgcaca tgcctccacc agttcatcac tgaatcgccc cgtttttttc tcctgtgcta
    22021 cctgctttcc ccacccggaa tatccccaac gagaaaggat tcgccccgta gatccttgta
    22081 aactcgcagg caagttccct ttaaatccgt attgcgtaaa agccccagct gccacctgtt
    22141 tattgagatt taatgacagg tcagcgtgtt taataaataa gtttgccgca tggagtaagc
    22201 cgctatcaat gtcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaat
    22261 gttcggcacc tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
    22321 tgatatagct ttcaacgtct gccagctcac tttttctctg attcatcaaa tggttaattc
    22381 gttcatggtc aggagaaaaa ctgacaattg ttctgcgcca taatttctcg gtatacacca
    22441 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
    22501 gattcgtccg ttgttcaatg cctcgtaagg taagaccgtc attcacgcta agtacgttga
    22561 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
    22621 cacggtcttc actgccgcaa agcagtatgc caacagcaga atcgataccg cgaccggcac
    22681 gccccacctg ttagtagtaa ccaactatcg aacctggcat ctggtaatga ataacaaacc
    22741 ccaaatccgg tttgtcaaag cccattccca gcgccgttgt cgcaaccagc actttaattt
    22801 tatttgccag caacgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
    22861 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttttcctt aaccactcgg
    22921 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
    22981 ggataaccgt tgctaaccat gccagacggg atgagtgttc acctaagact aaggcatcaa
    23041 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
    23101 ggatatcctc aacgacacgg ttattcgctg tcgcggttgt acccagaaca ggggtattcg
    23161 caggtagttg cgcaaaatat ctaatatacg tcggtaatcc gggcgaaaat cgtggcccca
    23221 atcagagatg cagtgcgcct cgtcgaccac aagcaagcca atatgatcgg ctataggata
    23281 taaaaccgtt tcgataaagt cctggtttgc caaacgttcc ggggaaacca gcaagcaatc
    23341 gacctcacct tgcagcaact tatcgctaat gcgctgccat tcttctctgt ttgtagaatt
    23401 caacgtttca gccgtaatac ctaaccgttc agcggcggca acctggttac gcatcagggc
    23461 aagcaaagga gagatgataa ttgtcgggcc agcgccgcga tcgcggaaga ttttactggc
    23521 gatgaagtac acagcacttt tcccccagcc agtgcgttgg acaaccagca gttttcgccg
    23581 ttggttcacc accgcgtcta tggcttccca ttgcccttcc ctgaaagtag cggtggtatt
    23641 tcctaacatc cgctgtaaaa gcaattcagc tgcgtgcttt tccatcttta tctcctgcga
    23701 tctttaggca gtgattagaa cggattattt gcaggtaaat ttgcgagaga gatcaaaaag
    23761 atgaagagag gagaaaagag aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca
    23821 gtaattaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga
    23881 tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac
    23941 cgaacaacca gaaaccagcg tcgttaacgt ggctgacaac aatcgaacca ccggcgatac
    24001 aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca
    24061 ccagtcccac cgccgttaaa caggcaacgg ttgcagaacc ctgaatgatg cgcactgcag
    24121 cggccagcac gaagcaggtg atggcaatcg gcaggcccat gccggttaac gcttcgccca
    24181 gtgccggacc tacgccagag tcaaccagca cctgtttgaa cacgccgccc gcaccaatca
    24241 ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt
    24301 ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg
    24361 cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct tccggcacaa
    24421 aacgcgcggc aatggttttc agccctacca gcaccagcgg cagcaggatc agcgacaggc
    24481 tgaatccgaa ggatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa
    24541 tatgcagctc aacgtaacgg ctgatgaagt taccccacag cggcccggca ataatcattc
    24601 ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg
    24661 atgccagcag cattggcgct ggccctggca ccaggaacgc cgccgcggcc gccacgcctg
    24721 cgaataatgg gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag
    24781 caacgctaat cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc
    24841 ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg
    24901 caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga
    24961 caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt
    25021 tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatt aaagccagga
    25081 aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg
    25141 ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc
    25201 ttgccacaac accttccagc ggttgatcga tatccaccac cagtacatcg gtttcgtccg
    25261 cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc
    25321 ctttgcgcgc tttcaggcgg ctttcaatca catcaaaatc gcctttcagg tagatgaaag
    25381 agagattcgg attaccttca cgcagcaagt cacgatagtg ttttttcaat gcagaacaga
    25441 cgatcagcga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca
    25501 accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc
    25561 gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac
    25621 ttgcgaccgc agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat
    25681 ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac
    25741 tgttatcggt aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa
    25801 gtgtataagt gtgagctact tcaaatttgt gggcttaaat agatccgccc ggtgacaagg
    25861 tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc
    25921 gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgca agtcgtggct
    25981 ccatcacctg accaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaactt
    26041 ttaaccccag acgctgacat tcaaacgccg cgccgaccgc cagatcatca ttagtacaga
    26101 acacgccatc cagctgcgga tattcccgcc gtgcctggcg aatcagttca ataccggaag
    26161 agtaagaaga agattgctca accatcacgc tatatggcac caggcctgca tccagcatcg
    26221 cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg
    26281 caatgtggcg atgcccgcga gcaataatgg ctgtggtcat ctggcgtgcc gcttcaaagt
    26341 tatcaaaacc gacggcgata tcgagacacg gcgactggct gtccatcaac tccaccaccg
    26401 ggatccccgc cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga
    26461 tcagtccgtc gatattccat gagagcattg attcgagacg ttcttgctcc atttccggtt
    26521 tataaccgta gtgggccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc
    26581 cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc
    26641 ggctggtggc gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa
    26701 gagccgcggc aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac
    26761 ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt
    26821 tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca
    26881 tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa
    26941 ttgcatcgca ttgtgctaac gacgctgcaa aaccccggaa gcgtacatca gcacgcgacc
    27001 ggagcgagcg aggaagcctg caaaaaggca ggcttcaaag atgccgttga gctttaaacc
    27061 ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc
    27121 tcatcccaga ttgccagacc atcgctgatc gaggctttca cgccgttaat ggtgacattg
    27181 cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca
    27241 tctttcagca acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatca
    27301 cgcgcatctg gcgaaagcac cagttgtttg ccctgcactg catcgaagcg acgctgttca
    27361 taacgcggcg taatgccgtt ttcttccggc atgatccaga tctgatacag gtgcagacgc
    27421 tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg
    27481 aactcgcccg ccggaacctg ctctttattg cccatgctgt cctggtgctc aacagtacct
    27541 tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct
    27601 gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg
    27661 taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca
    27721 cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg
    27781 cttagtgtgg acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa
    27841 aaaaattgaa ctattcgagg ggccatcaaa cttacttcgc gagagtcact gtggagggag
    27901 aggcttgctc gaatccacgt tcaagtattt ccagattcgt aagaacttca gattccttga
    27961 cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat
    28021 cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca
    28081 gcacaccgac cgaatcatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca
    28141 ggctggtttc ctgctggtcg ataccatatt taataaacga acctttctta ccgtgaacga
    28201 taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct tttagatcgc
    28261 cataaaacag ttgcgcttcg aaagtatcgt caggattggc tttattgcgc aggctgcgga
    28321 tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca
    28381 caccaaggcc atagaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc
    28441 ggtaatagtc aaaatggctt tccacttcga caatctctcc cagcttgccg ctttcaatcg
    28501 cttttttcgc cgtcaggaag caggaatcaa agcgacgatt ctgatatggc gtgacggtca
    28561 gccctttgct tttcgccagt gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg
    28621 gtttttcgac cagcacattt ttcccggctt ccatcgcgcg cttcgcgtac tcgaagtggc
    28681 tgtccgcatg ggtgcagaca acaaccagct taacatcggg atcgtttagc acttcgttga
    28741 gatcgctggt gaaatggata tgagagtaaa tgggagcttg ttcttccggc tttgcatggc
    28801 ggcgaaaaat atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt
    28861 aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct
    28921 ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca
    28981 taacgaacac aagcacttcc cgaggataaa ttgagaacga aagatcaaaa aaaaagccag
    29041 cacccggctg gctaagtaat actggaagca atgtgagcaa tgtcgtgctt tcaggttctc
    29101 cgcgagggtc ttcctgatcg cgagacaata ataatcattc tcattcgcac ttgtccaaca
    29161 ctttttgcaa aaaaatgcat ttgactcgca ttcgaaagtc aatgatgttg aaagggacat
    29221 ttaccccaaa gaggacaaag gaatgagtga gatagtgata cgccatgctg aaacacggga
    29281 ttacgaggcc atcaggcaga ttcacgccca gccggaggtg tattacaaca cactacaggt
    29341 gcctcatcct tccgatcata tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca
    29401 actcgtcgcc tgtattgatg gagacgtcgt gggccatctc accattgacg tgcaacagcg
    29461 cccacgccgc agtcatgttg ccgattttgg tatctgtgtc gacgcccgct ggaagaatcg
    29521 cggcgtcgcc agcgccctga tgcgagagat gattgaaatg tgcgacaact ggttgcgggt
    29581 agatcgtatt gagctaacgg tgttcgtcga taacgcgcca gcaattaagg tctataaaaa
    29641 attcggcttt gagattgaag ggactggcaa gaagtacgca ttacgtaatg gtgaatatgt
    29701 tgatgcgtat tttatggcgc gggtgaagta aagaatattc tcaaattgcc tgttaccaat
    29761 gtgaataatt agttttgttc atctaatttg agtttttcct tatattacca ccatcatttt
    29821 gaaacaattt gtaaca
//
LOCUS       DAZFKY010000041        29604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFKY010000041 DAZFKY010000000
VERSION     DAZFKY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..413
                     /locus_tag="ACHKCL_001618"
     CDS             <1..413
                     /locus_tag="ACHKCL_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5762614.1"
                     /translation="DVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKN
                     SLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSG
                     HGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(456..1316)
                     /gene="rsmI"
                     /locus_tag="ACHKCL_001619"
     CDS             complement(456..1316)
                     /gene="rsmI"
                     /locus_tag="ACHKCL_001619"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5762615.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHKCL_001620"
     CDS             1381..3417
                     /gene="lpoA"
                     /locus_tag="ACHKCL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5762616.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3375..3770
                     /gene="yraN"
                     /locus_tag="ACHKCL_001621"
     CDS             3375..3770
                     /gene="yraN"
                     /locus_tag="ACHKCL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5762617.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3790..4380
                     /gene="diaA"
                     /locus_tag="ACHKCL_001622"
     CDS             3790..4380
                     /gene="diaA"
                     /locus_tag="ACHKCL_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5762618.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4390..4965
                     /gene="dolP"
                     /locus_tag="ACHKCL_001623"
     CDS             4390..4965
                     /gene="dolP"
                     /locus_tag="ACHKCL_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5762619.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHKCL_001624"
     CDS             complement(5079..6119)
                     /gene="yraQ"
                     /locus_tag="ACHKCL_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5762620.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHKCL_001625"
     CDS             complement(6192..6827)
                     /gene="yraR"
                     /locus_tag="ACHKCL_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5762621.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHKCL_001626"
     CDS             6955..7473
                     /gene="yhbO"
                     /locus_tag="ACHKCL_001626"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5762622.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHKCL_001627"
     CDS             complement(7453..7896)
                     /gene="yhbP"
                     /locus_tag="ACHKCL_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5762623.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHKCL_001628"
     CDS             7947..8249
                     /gene="yhbQ"
                     /locus_tag="ACHKCL_001628"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5762624.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHKCL_001629"
     CDS             complement(8236..8739)
                     /gene="yhbS"
                     /locus_tag="ACHKCL_001629"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5762625.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHKCL_001630"
     CDS             complement(8733..9257)
                     /gene="ubiT"
                     /locus_tag="ACHKCL_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5762626.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHKCL_001631"
     CDS             9466..10461
                     /gene="ubiU"
                     /locus_tag="ACHKCL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5762627.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHKCL_001632"
     CDS             10470..11348
                     /gene="ubiV"
                     /locus_tag="ACHKCL_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5762628.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHKCL_001633"
     CDS             11429..12436
                     /gene="yhbW"
                     /locus_tag="ACHKCL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO5762629.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKARHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHKCL_001634"
     CDS             complement(12553..13797)
                     /gene="mtr"
                     /locus_tag="ACHKCL_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5762630.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHKCL_001635"
     CDS             complement(13951..15840)
                     /gene="deaD"
                     /locus_tag="ACHKCL_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5762631.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHKCL_001636"
     CDS             complement(15833..15913)
                     /gene="yrbN"
                     /locus_tag="ACHKCL_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5762632.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHKCL_001637"
     CDS             complement(16020..16904)
                     /gene="nlpI"
                     /locus_tag="ACHKCL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5762633.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHKCL_001638"
     CDS             complement(17013..19148)
                     /gene="pnp"
                     /locus_tag="ACHKCL_001638"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5762634.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHKCL_001639"
     CDS             complement(19395..19664)
                     /gene="rpsO"
                     /locus_tag="ACHKCL_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5762635.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHKCL_001640"
     CDS             complement(19813..20757)
                     /gene="truB"
                     /locus_tag="ACHKCL_001640"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5762636.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHKCL_001641"
     CDS             complement(20757..21158)
                     /gene="rbfA"
                     /locus_tag="ACHKCL_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5762637.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHKCL_001642"
     CDS             complement(21323..23995)
                     /gene="infB"
                     /locus_tag="ACHKCL_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5762638.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHKCL_001643"
     CDS             complement(24020..25507)
                     /gene="nusA"
                     /locus_tag="ACHKCL_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5762639.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHKCL_001644"
     CDS             complement(25535..25987)
                     /gene="rimP"
                     /locus_tag="ACHKCL_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5762640.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26194..26270)
                     /locus_tag="ACHKCL_001645"
     tRNA            complement(26194..26270)
                     /locus_tag="ACHKCL_001645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26234..26236),aa:Met,seq:cat)
     gene            26618..27961
                     /gene="argG"
                     /locus_tag="ACHKCL_001646"
     CDS             26618..27961
                     /gene="argG"
                     /locus_tag="ACHKCL_001646"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5762641.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHKCL_001647"
     CDS             complement(27969..29594)
                     /gene="yhbX"
                     /locus_tag="ACHKCL_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5762642.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7138 a         8080 c         7411 g         6975 t
ORIGIN      
        1 gcgatgttat taaggttaat tttggattca tcaatggtca gaagtttacc acccgcaatg
       61 ccatgccaga cggttttact ccagtagact ttgatatcac ttatgactgt ggtgatactt
      121 caaagattaa aaactcgttg caaatgcgca tcgacggtac aactggggta gtagaccagt
      181 acaacctggt cgccagacga agaagttcag acaatgcgcc cgatgtcggt attcgtattg
      241 aaaatctcgg cggcggagtt gcaaatattc cttttcagaa cggtatcctt cccgttgatc
      301 cttccgggca tggcaccgtc aatatgcgcg cctggccagt taatctggtc ggtggtgagc
      361 tggaaacagg aaaatttcag ggcacagcca ccattaccgt catcgtgcgg taaaagaaga
      421 cgttgcgttt tgattaacgc aacgcttcgg cactcttacc cctgctgctc cagcgcatac
      481 ttatacagcg catttttctt cacgccgtga atttctgcgg ccagcgccgc cgcttttttc
      541 agcggcagtt ctgcctgtag cagcgccagc gtgcgcaggg catcggcggg taagtcttct
      601 tcctgtgctt tatgaccttc gacaatcagc accatttcgc ctttgcgacg gttttcatct
      661 tcctttaccc acgccagcag ctcgccaacg ggcgcgccgt gaatggtttc ccaggttttg
      721 gtcagctcac gcgccagaac cacgtagcgg gattcgccta ataccgcaac gatatcttcc
      781 aggctatcta acagacggtg ggtagattca taaaaaatca gcgtgcgcgg ctccgcttca
      841 atggctttta gcgcatcacg gcggcctttt gatttggcag gtaaaaagcc ttcgtaacag
      901 aaacggtcag agggtaaacc cgcggcgctt aacgcagtga tagcagcaca cggtccgggt
      961 agcggcacca cacgaatccc cgcttcacgg caggtacgca ccagatggta gccaggatcg
     1021 ttaattagcg gcgttccggc atcggaaacc agcgcaatgt tttgcccctc ttgcagcttc
     1081 gccagcagcg tttcggcttt ttgttgttcg ttatggtcgt gcagcgcaaa caaccgggca
     1141 ttaatcccaa aatgttgcag caataaaccg gtgtgacgag tatcctcggc ggcaatcaga
     1201 tcaacggcct gtaatacctc taacgcacgc tgggtgatat ccgccagatt gccgattggc
     1261 gtcggtacaa tgtaaagctg gccctgagaa ttatccgccg attggtgttg tttcattgtg
     1321 tcgtccgtat tgccgattta atattgagca ttgcgtaaaa aaaatatcac tggatacatt
     1381 atggtaccct caacattttc tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca
     1441 gccctgattt tcgtcggttg tggcacccat actcccgatc agtccactgc ttatatgcag
     1501 ggcacggcgc aggctgattc tgccttttat cttcagcaga tgcagcaaag ctctgatgat
     1561 accaggatca actggcaatt actcgccatt cgtgcactgg tgaaagaagg taaaaccggg
     1621 caggcggttg agttgtttaa ccaactaccg caagaactga acgattctca gcgtcgcgag
     1681 aaaacactgc tggcggtaga gattaaactg gcgcagaaag attttgctgg cgcgcaaaac
     1741 ttgctggcga aaattacacc cgccgattta gaacaaaacc agcaagcccg ttactggcag
     1801 gcaaaaatcg atgccagcca ggggcgtcct tccattgatt tactgcgcgc gttaattgct
     1861 caggaaccgc tgcttggcgc gaaagaaaaa cagcagaata ttgatgccac ctggcaggcg
     1921 ctctcctcca tgactcagga acaggcgaat acgctggtga tcaacgccga cgaaaatatt
     1981 ctgcaaggct ggctggatct gcagcgcgtc tggtttgata accgtaacga tcccgacatg
     2041 atgaaagccg ggatcgccga ctggcagaaa cgttatccga acaatccggg cgcgaaaatg
     2101 ctgccaacgc agttggttaa cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc
     2161 ctgctgttgc cactgaatgg ccaggcagcg gtatttggtc gcactattca gcaaggcttt
     2221 gaagcggcga aaaatatcgg cactcagcca gtggcggctc aggtagctgc cgcacctgcc
     2281 gcagacgtag cagaacaacc tcagccgcaa actgcggatg gcgttgccag cccggcacaa
     2341 gcctcggtta gcgatctgac cggtgatcag cctgcggccc agccggtgcc tgtaagcgcc
     2401 ccggcgacaa gcaccgcagc ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc
     2461 tacgatacct catcacaacc acttagccag atcttaagcc aggttcagca ggatggcgcg
     2521 agtattgtgg ttggtccgtt gctgaaaaat aacgttgaag agttgctgaa gagcaacact
     2581 ccgctgaacg tactagcact gaaccagccg gagaatatcg aaaatcgcgt caatatttgt
     2641 tacttcgcgc tttcaccgga agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag
     2701 ggtaaacaag cgccgctggt gctgatccca cgcagttcat tgggcgatcg cgtagccaat
     2761 gcgtttgcgc aagagtggca gaaattgggc ggcggcaccg ttctgcaaca aaaatttggt
     2821 tccaccagcg aattacgcgc gggtgttaac ggcggttctg gtattgcttt aacgggtacc
     2881 ccgattactc ccagagcgac aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa
     2941 accacgccaa ccgatgacca gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt
     3001 gtggcaacgc cgggtgaaat cgcttttatt aaaccgatga tcgccatgcg taacggtagc
     3061 cagagcggtg caacgctgta cgccagctcc cgcagtgcgc aagggaccgc tggcccggat
     3121 ttccgtctgg agatggaagg cttgcagtac agcgaaatcc cgatgctggc gggcggtaat
     3181 ctgccgttaa tgcagcaggc actcagcgcg gtgaataacg attattcact ggctcgcatg
     3241 tatgcgatgg gcgtcgatgc ctggtcgctg gcaaatcatt tctcacaaat gcgccaggtt
     3301 cagggttttg aaatcaacgg taataccgga agcctgacgg ctaacccgga ttgcgtgatt
     3361 aacaggaagt tatcatggct acagtaccaa caaggtcagg tagtccccgc cagttaacca
     3421 ccaaacagac cggcgatgcg tgggaagtac aagcgcgtcg ctggctggaa ggcaaaggac
     3481 tgcgatttgt cgccgctaac gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg
     3541 aaggccggac caccgttttt gtcgaggtgc gttaccgccg ttctgcgctt tatggcggcg
     3601 cggcagccag tgtgacccgc agcaaacaac acaaattatt acagactgcc cgcttgtggc
     3661 tcgcgcgtca taatgggagt tttgatactg tggattgccg gttcgatgtg gtagccttca
     3721 ccgggaatga ggttgagtgg attaaggata cctttaatga ccactcataa ttaaggttta
     3781 aggattagcg tgcaagaaag aattaaagct tgcttcactg aaagcattca aactcaaatt
     3841 gcggcggcag aggcgcttcc ggatgccatc tcccgtgcgg ccatgacgct ggttcagtct
     3901 ctgctcaatg gcaacaaaat cctctgttgt ggtaatggaa cttccgctgc caatgcacag
     3961 cattttgctg ccagcatgat caaccgtttc gaaacggagc ggcccagctt acctgccatt
     4021 gcactaaata ccgataatgt tgtcttaacg gcgattgcca acgatcgctt acatgatgaa
     4081 gtgtatgcaa aacaggtgcg ggcgctgggg catgcgggag atgtattgtt agccatttcc
     4141 acccgtggca acagccgcga tatcgtaaaa gcagtagaag ctgccgttac gcgtgatatg
     4201 accattgtgg cattgaccgg ttatgacggc ggtgaacttg caggtttgtt agggccacag
     4261 gatgtggaga tccgcatccc ttcgcatcgc agtgctcgca ttcaggaaat gcatatgctg
     4321 acggtaaatt gcctgtgcga tctgatcgat aacacgcttt tccctcacca ggatgattaa
     4381 ggagaataca tgaaggcatt atcgccaatc gcagtcctta tttccgcgct gctgttgcaa
     4441 ggttgtgttg ccgctgccgt agtgggtact gctgctgtgg gtaccaaagc cgcaactgac
     4501 ccacgcagtg tcggcaccca ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca
     4561 ttgtcgaaag acgaacagat taagaaagaa gcgcgcatta atgtaacggc ctatcagggc
     4621 aaagtgctgc tggttgggca gtcaccaaac gctgaacttt cggctcgcgc caaacagatt
     4681 gctatgggcg tagacggtgc caacgaagtg tataacgaga ttcgtcaggg ccagccgatt
     4741 ggtctgggcg aagcgtctaa cgatacgtgg atcaccacca aagtgcgttc gcagctctta
     4801 accagcgacc tggtgaaatc gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc
     4861 ctgatggggc tggtgactga acgtgaagcg aaagcggcgg cagatattgc cagccgggtg
     4921 agcggcgtga agcgggtaac tacggcgttt acgtttatta aatagcagcc ccttgtaatg
     4981 cctgatgcga cgcttgccgc gtcttatcag gcctacaacg atgcaaaccg taggtcggat
     5041 aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc agaataacag cgccagcccg
     5101 ccgacaatca cgcctgacac tgccaccatc gcccccgtca gccataaggc tttcgccggg
     5161 aacgctttgc gcagcataat cagtgacggc aaactcaccg ccgggagcgt catcaacaat
     5221 gccagcgccg gagcagttcc catacctgcc agcatcatcg tttgtacaat cggaatttct
     5281 gctgccgtgg gaatgacaaa caagcaccct gctaccgcca tcgccaccac ccacatcagg
     5341 ctgttatcga cagcaccatc ggcatggggg aataaccaga cgcgagcggc acccaacacc
     5401 agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa gcgtccatag cgccctgccc
     5461 cagcgactaa aaaatccgcc ctgtgcttcc ggtatgtcaa tttcgaccgg tgcttgcgtt
     5521 tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc
     5581 ccggccacca gacgaatcgc cgcaaaaccc cagccgagga caaaccccat aaacaccagc
     5641 gtcgccgggt ttaacaccgg attgcccatc cagaatgcca gcgcaccgcc catcgacacc
     5701 tgttggcgac gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcctggc
     5761 agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac
     5821 aaccagtcac gcgggatcag cacctgaatc aacgaaccga ggatcacccc cagcaccgcc
     5881 gctttccata ccgcgaggaa atagatcatc gcgtaatcca acgccgcctg ccatggatta
     5941 gcatccgcct gcgcaaggat agatttaccg atactgtggg tttcggcagc agtaaacgct
     6001 ttgccgtagt aaggttgcca tttcacatac cagagaccgg caatgacaac gagaaagaaa
     6061 agcgcgggtt tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata
     6121 gcattcccca ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt
     6181 cttgggaata attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacacct
     6241 tcatgctcgg gccgcatcgc ctctgccagc atgacccgcg ccacgtctct cgcatcaatg
     6301 gatttccagt taccgggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc
     6361 tgtttgctac gatcgcccag taacatcgac gggcgagcaa tggtcaattt cggccagttc
     6421 tgggcgatta atgcttcttc catctcccct ttgacgcggt tatagaaaaa cggcgagtgg
     6481 gcattggcac ccatagcact gaccaccagc atatgctgcg cgcccagtcg ccgcccggtt
     6541 aatgcggtat ccactaccag cgtgtaatcg gcatgaataa acgcctcttt gctccccgct
     6601 tctcgccgcg tggtgccgag acaacaaaac acaatgtcga tgggatcggt gacctgcgcc
     6661 agcgcgtcag tcagttgcgg atcatgggga ttaaataccc cgggcatatc gcccaacgga
     6721 cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt taatcaacat ccgcagcagg
     6781 tgaccgccca ccagccccgt tgcgcctgta atcagtacct gactcatctt cgctccttta
     6841 cagaattgtc tgccttgcgc tccacggctc atgcaccagg cttaatagac catgaggtaa
     6901 ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt
     6961 aagaaaattg ccgttttaat cactgatgaa tttgaggatt cagaatttac ttcacccgca
     7021 gacgagttcc gtaaagccgg acacgaagtg attaccattg aaaaacaagc gggtaaaacg
     7081 gtgaaaggca aaaaaggaga agccagcgtg accatcgata aatccatcga tgaagtgacg
     7141 ccagcggagt ttgatgccct gttgctaccg ggcggtcatt caccggatta tctgcgtggc
     7201 gacaaccgtt ttgtcacctt tacccgtgat tttgtgaata gcggcaaacc tgttttcgcc
     7261 atctgtcacg gtccgcagct gctgatcagc gccgatgtga ttcgggggcg caaactgacc
     7321 gcggttaaac cgatcattat tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa
     7381 gtcgtggtcg ataaagatca gctggttacc agccggacac cggacgatct gccagcgttt
     7441 aaccgcgagg cgttacgcct gctcggtgcc tgagccgcgt agccaaatca tttttttacc
     7501 aaagcccagc gtgttgtcgg tgaatttgat ttcatcgggc cggatttccc acaccggggc
     7561 cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc
     7621 ctcaccttcc agcctgcgga tctcaccttt aaactgcacg ccgcgaatta acgctaccgt
     7681 tttcggctga ccgtttaccg ttccggcaac cgcagcctgc ggcccactca tctgcgcgtg
     7741 gcgcgttttt tcttccgtca aaatgtagaa ggcgactttc tgcacatcaa acagataaaa
     7801 ggcattggcg caccagagtt ccccttcctg ctgcacacac caggtgacaa catgttgttt
     7861 tgccagccaa cggctgatgg cggtgagtgt ttccattgct gttctctttt atactgtggg
     7921 cctgacctta acatgcgacg cagacgatga caccctggtt tctttacttg atccgtaccg
     7981 ccgacaataa gctttatacc gggatcacca cggatgtcga acgccgctat cagcagcacc
     8041 aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga actgacgctg gcgttttctg
     8101 cgccagtggg cgatcgttcg ctggcgttac gggcggaata tcgcgttaag caactgacca
     8161 aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt tgcaaagctg ttaagcagtc
     8221 tgcaaacccc ggagattaaa agcgattgaa atgctcgtga tactcaacca gaccagttac
     8281 gccattcaac gcgtcatccg ctaaacgatg tacctggaag gcgctttcag tgcccggcca
     8341 gcggcaacgc agatcgtgat gcgccgccag ttcaaagccg aaacggctgt atagcgccgg
     8401 atcgcccagc gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata
     8461 gaccagttgg cgtgccagcc cctgtccacg gtatttttca tctaccgcca gcggtgccat
     8521 gccgacccat tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat
     8581 gacctgacct tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag
     8641 atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat
     8701 acccggcgca tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt
     8761 ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc
     8821 atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac
     8881 agccccagct ccgtatcgcc ttcaatcacc agccgacgct ggaagaagag cgtatccgga
     8941 tcttgtttac gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca
     9001 gcttgcgcgt tctggctgac caccagtttg ccattcacca ccgaggtaaa ccattgcagg
     9061 tcaatatcac gcacatgaat acttaaccag cggccttcaa gaaactccag ctcaccatca
     9121 tccagcgcct ggcggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca
     9181 aatggcgtca gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa
     9241 cgcagtttat ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat
     9301 cagaaaaata acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa
     9361 tacaacttta actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt
     9421 cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc
     9481 cctgccggaa atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat
     9541 atcgggctaa aagatgatac caatgcccgt cacttcgccg gccttaactt taccgagaaa
     9601 aaattgcagg aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt
     9661 aacacttttg cgcatccgga cggttacgcc cgttggcagc gtgccgtgga tatggcggcg
     9721 cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca tgctagagta cgccgccgag
     9781 cgttacccgc atattgagcg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg
     9841 attaactttt atcatcgcca ttttgacgtt gctcgcgtgg tgctgccgcg cgtgttgtcg
     9901 attcatcagg tgaaacaact ggcacgggtc acacctgtcc cgctggaagt ctttgctttc
     9961 ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt cgtcgtatct gacgggtgag
    10021 tcgcccaaca ccgtgggcgc gtgttctccg gcccgtttcg tgcgctggca gcaaacgccg
    10081 caggggctgg aatcccgcct gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac
    10141 gcaggttatc cgacgctatg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg
    10201 ctggaagaac caaccagtct caataccctg gaactgctgc cggagttaat ggcggcgaat
    10261 attgcttcgg tgaaaattga aggccgccaa cgtagcccgg cgtatgtcag ccaggtggcg
    10321 aaagtctggc gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa
    10381 agcgcgtgga tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg
    10441 tatcaccgta aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt
    10501 actggccaaa agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg
    10561 tgatttatct tggcgaagcg gtttgcagca agcgccgggc aaccaaagtt ggcgactggc
    10621 tggagatggc aaaatcgctc gccggaagtg gtaagcagat tgtgctctcc acgctggcgc
    10681 tggtgcaggc atcatctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc
    10741 tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg
    10801 tcgccggaca cgcgctgaac tgctataacg cggtgacgct gaaaatattg ctcaaacagg
    10861 gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta
    10921 atcagtgcga tgagctgggc attcgcaacc agtttgaagt tgaagtcctg agctacggtc
    10981 atctaccgct ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag
    11041 atgagtgtga aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa
    11101 accaacaagt gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg
    11161 gtaacgagct ggcatccatg cagggcctgg ttgatgtggt ccgcctgtca ccgcagggta
    11221 ctgacacttt cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcaccactgc
    11281 cgttgacggc gaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc
    11341 aagcctaagt aaatagctca ctttgttaac aactttaact actctttaat gcagtattaa
    11401 agattaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact
    11461 cgatctggcc cccattcccg aaggttcttc agcgcgagat gcattctccc actctctcga
    11521 tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa
    11581 tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac
    11641 caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat
    11701 tgcagaacag ttcggcacgc tcaatacact ctatccgggg cgaatcgatt tggggctggg
    11761 tcgtgctccg ggtagtgacc agcgaaccat gatggcgcta cgtcgtcata tgagcgggga
    11821 tattgataat ttcccccgcg atgtcgcgga gctggtggcc tggtttgacg cccgcgatcc
    11881 caatccgcat gtgcgcccgg taccaggcta cggcgagcaa atccccgtgt ggttgttagg
    11941 ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgtttg cgtttgcctc
    12001 acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc
    12061 gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ctgccgacag
    12121 caaccgcgac gctgaatttc tgtttacctc aatgcagcaa gcctttgtga agctgcgccg
    12181 tggcgaaacc gggcaactgc cgccgccgat tcaaaatatg gatcagttct ggtcaccgtc
    12241 tgagcagtat ggcgtgcaac aggcgctgag tatgtcgctg gtgggcgata aagcgaaagc
    12301 gcgtcatggt ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggttaacgg
    12361 gcagattttc gaccaccagg cgcgcctgca ttcgtttgag ctggcgatgg atgttaagga
    12421 agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg caacgctgtc
    12481 ccacgtaggc ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat
    12541 aaggcccgct gattactgat acaccggcag caaattaaag ctcgataaga tatggaccag
    12601 tgcgttgcca acgccaaata ccagaatcag cgtaatcatc ggcttgccgc cccagacgcg
    12661 gaatttcggg ctgccaaagc gtttacgcga tgcgcgggcc agcagtgccg gaacaattgc
    12721 cgcccagatg gtagctgcta aaccagcata accaatggcg tacaggaatc cgttcgggaa
    12781 cagcagacca cccacaacag gaggggcaaa ggtcagcaat gccgttttca agcggcccac
    12841 agccgagtcg tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgttacgcc
    12901 gaggaacgaa ctcgctaccg caaagtttga gaacacgacc agcagcagat ccagactacg
    12961 gctgttcagt acgccgctta acgcctgtac cagcacatca atattaccgc ccttctctgc
    13021 aatatcgata aactccggac gcgggatgtt acccatcgtc gccagcaacc agatggtata
    13081 cagcgccagc gccatcagcg taccgtacac cagacatttc acgatggttt tcggatcttt
    13141 gccgtaatac ttcatcaggc ttggcacgtt accgtgataa ccaaacgatg ccagacagaa
    13201 cggcaaggtc atcaacagat acggtgcata agacgcattg ctttcagcga cgttgaacaa
    13261 tgtcgcaggc tgcacatgcc ccagcaggct accgaaggtg aggaagaagg taatgacttt
    13321 cgcccccaat acaatcgctg tcatgcgact gacggcttta gtgctcaacc acaccacaaa
    13381 cgctaccagc aatgcaaaac caaaacccgc cgcccgtgcc gggacgttta gtgacatctc
    13441 tgcgaaggtg tgatgcagaa tcgaaccact ggcagaaata taggcatagg tcaggatata
    13501 gagcacaaag gcaatggaaa tgccgttgac cacgttccag cctttgccca gcaaatcttt
    13561 ggtgatggtg tcaaaactcg aaccgattct gtaattcagg ttagcttcca gaatcatcaa
    13621 gccggaatgc agcatacaga accaggtaaa gatcagcgcc gccattgacc agaaaaacca
    13681 cgccccggac atgaccactg gcagagaaaa catccctgcg ccaataatgg tgccgccgat
    13741 aatcaccacg ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag
    13801 ggcttctctc cagtgaaaaa tagtgcgact gcgttgttat gtattgcact gtaccagtac
    13861 gcgagtacaa aagacagaaa aaaagccccg atggttcaaa tcggggctgt atatattatt
    13921 ttacagactg tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc
    13981 ggtagagtca tcacgacgcg gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc
    14041 atcaccacga ccaccttcac ggcgttcacc gctgaagtta cgaccacctt cacgacgctc
    14101 gccactgaaa ccacgaccac cgccacgacg ctctccgcca gtatgcggct gtgcatcgcc
    14161 cagtaattgc atattcatcg gcttgttcag aatgcgagta cgtgtaaagt gttgcagcac
    14221 ttcacccggc atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttaatgtt
    14281 accgatgtaa cggctgctga tgtcgccttc gttagcaatc gcaccaacga tatgacgaac
    14341 ttcaacacca tcatcgcggc ccacttcaat acggtacaac tgcatgtcac caacatcacg
    14401 acgttcacga cgcggacgat cttcacggtc gccacgcgga ccacggtcgt tacgatcgcg
    14461 cggaccacgg tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg
    14521 aacaatcaga gtacgttcac cctgtgccat tttcagcagt gcggcggcca gagtttcgag
    14581 atccagctct tcaccttctg cagtcggctg aattttgctc agcagtgcgc ggtattgatc
    14641 cagatcgctg ctttccagct gctgctgtac tttagcggcg aatttttcca gacggcgttt
    14701 gcccagcagt tctgcgttcg gcagttctac ttccggaata gtcagcttca tagtacgttc
    14761 aatgttgcgc agcagacgac gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg
    14821 acccgcacga ccggtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc
    14881 gtagttaacc accaggctga tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc
    14941 aatcaggatg tccagacgac catctttcag gcgttccagt gtctgctcac gcagcgcctg
    15001 gttcatgtca ccgttcagcg cggcgctgtt gtagccgtta cgctcaagag cttcagccac
    15061 ttccagagtc gcatttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc
    15121 caggaaacgt accagtgctt cgtttttacg cataccccag acagtccaat agctctggct
    15181 gatgtcagga cgggtagtca cgctggactg aatgcgcact tcctgcggct ctttcataaa
    15241 gcggcgggta atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg
    15301 accttccggg atctgcgcca taatagtttc aacgtcttcg atgaagccca tgcgcagcat
    15361 ttcgtcagct tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt
    15421 caggtggtcc agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc
    15481 gcgtaattgc acgtcataac gctggccgcc gtacagggca accacattta cgccgcgcat
    15541 gtgtttagag aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc
    15601 tagcaccaga atctgtggtg ctttcagctc aggatcaaga ttttgcagca gaggtaaaga
    15661 gaatgctgca gtttttccgc tccccgtctg ggccataccc agtacgtcgc ggccattcag
    15721 cagatgtgga atacactccg cctgaattgg agatggtttt tcgtaaccca gatcgttaag
    15781 ggcttcaagg ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat
    15841 gtagtacgtg tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat
    15901 cagcaatttt cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac
    15961 gccctcaccc gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc
    16021 tattgctggt ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac
    16081 aatgcgtatc ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt
    16141 gccgtggcgc tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt
    16201 tcactgagat gctcagcgag cgaggtgtta tccgttgcgt ccgccttgag cctttccatt
    16261 aacgtttgtt cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc
    16321 ttatccgatt tttcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc
    16381 tgctcggcga gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac
    16441 gccagcagat catcttgcgc taacttgtca cgaccaccgt aatataatgc gatcccgcga
    16501 ttcaagtgcg cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag
    16561 gcagcatcaa aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc
    16621 atatccggtc ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg
    16681 agactatcat acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa
    16741 gcccgactgg caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt
    16801 tgcaatggta ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc
    16861 gtaagtgctg tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga
    16921 ccacggttga atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag
    16981 ccccccgccg cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct
    17041 tccggtgctg cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg
    17101 ccctggcgat caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg
    17161 gtcactttct caacgcgttt gtcagcgatt tgagagatgt ggaccagacc ttctttaccg
    17221 ccgccgatgg caacaaatgc gccgaagtca acgatacggg tcactttacc agtgtagacg
    17281 cggcccactt caatttctgc agtgatctct tcgatacgac gaatagcgtg tttcgctttc
    17341 tcgccatcgg tcgctgcgat cttcacagta ccgtcatctt cgatttcgat ggtggtgcca
    17401 gtttcttcgg tcagggcacg gatcacagag ccgcctttac cgataacgtc tttgatcttg
    17461 tccgggttga tcttgatggt atggatacgc ggagcgaact cagagatatc gccacgtggc
    17521 gcgttgatcg cctgttccat tacacccagg atgtgcagac gcgcaccttt agcctggttc
    17581 agtgcaacct gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcagtgca
    17641 gagataccgt cgcgggaacc cgcaactttg aagtccatat cgcccaggtg atcttcgtcg
    17701 cccaaaatgt cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata
    17761 cccgcaacgg cagctttgat tggcacacct gcgtccatca gcgccagaga cgcaccgcac
    17821 acggaagcca tagaagaaga accgttggat tcagtgattt cagacacaac acgtacggtg
    17881 tacgggaatt tgtccatatc cggcattact gccagcacgc cgcgcttcgc cagacgaccg
    17941 tgaccaattt cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga
    18001 gggaagttgt agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc
    18061 tgcgcgtcac gagcagtacc cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg
    18121 aacagcgcag aaccgtgagt acgcggcagc acgccagtac gcacatccag accacggatc
    18181 atatcttttt cacgaccatc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg
    18241 tttttctcga tcgcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcgtcttca
    18301 gcaagcagcg tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt
    18361 ttgtcggtga tgcggtaagc atcgctcaga cgagcttcag ccagtgcagc aacgcgcgcg
    18421 ttcagcgctt cgtttaccgg ctccggctgc cagtcccaac gcggtttacc ggcttctttc
    18481 accagttcat tgatgttctg aataacaacc tgctgttgtt catgaccgaa cactactgcg
    18541 cccagcatct ggtcttcgct cagcagttca gcttcagatt caaccatcag tacagcggct
    18601 tcagtacccg caacaaccag atccagtttg ctctctttca gctcgtcctg agtcgggttc
    18661 agtacgtact ggtcattgat gtaacctacg cgggcagcac caatcgggcc attgaacgga
    18721 ataccagaca gagacagtgc tgcggaagca ccaatcatcg cgacgatatc cgggttaacc
    18781 tgcgggttaa cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg
    18841 aacagcgggc gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttgga
    18901 cgaccttcac gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga
    18961 tagttaacgg tcagtgggaa gaagtcctga cctggtttgg cttttttctg gccaacaacg
    19021 gtaacaaata ccgcggtgtc atccatgcta accataacag cggcagtagc ctgacgagcc
    19081 atcatgccgg tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga
    19141 ttaagcaatg taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata
    19201 cccgatcttc tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa
    19261 agcctctcat tagccgcgcg aacctctgca acggaagatc attcatagca acaatacatt
    19321 agtttccagt gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccccttttct
    19381 gaaactcgca agaattagcg acgcaggccc aggcgctcga tgagccgggt gtaacgtgct
    19441 acgtctttac gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga
    19501 ccacgacggc tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc
    19561 tgtgcagtca gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca
    19621 aactcagaaa cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt
    19681 atatagaatg aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt
    19741 gttaaacaat ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca
    19801 agacggttaa cattacgccg ggtattcaac caccagacga cgaggcgcaa cgcggccttc
    19861 atcgtcaatt tcgcccatac cgataaactt gccgttctca ccttccgtaa cgcgaaccag
    19921 tccttccagc ggcgcgccag atgtacgaac cgggttacca tttttgaagt aaacagaaga
    19981 cgttaacgga agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa
    20041 tggatcaagc aactccgcag ccggaatatc ctgctgttcg gcttgttcga ccagttcacg
    20101 caggtgctcc agggtcacca tccgttcaac cggatattta cttaccgcca gacgacgcag
    20161 gtaaatcaca tgcgcgccac agccgagttt ttcacccagg tcatcaatga tggtgcggat
    20221 ataagtgcct tttgagcagt gaatttccag ctccagctca ttgccttcat ggcgaataaa
    20281 cagcaattca taaacggtaa ttggacgcgc ttcacgcgga acttcaatgc cctgacgcgc
    20341 atattcgtac agttttttgc cctggtattt cagtgctgaa tacatcgatg ggatctgttc
    20401 gatatcgcca cggaaagtat ccagtgccgc cgccagttgt tctgcactaa aggttaccgg
    20461 acgctcttca acgatttgtc cgtcggcgtc agaagtatcg gtacgctgtc caagacgcgc
    20521 aatgacccga tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc
    20581 gaggcaaatc ggcaacatgc cggtcgccag cgggtccagc gcaccggtat gcccggcacg
    20641 attggcgtta tatatacgtt tcactttttg cagcgcatcg ttgctggaca taccctgcgg
    20701 tttatccagc aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag
    20761 tcctccttgc tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc
    20821 accaggtttg acatacgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc
    20881 ggcacgatac gcaggcgcat cgctttaccc agcaggctgc ggatgaaacc agaagcttcc
    20941 tgcaacgctt tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaaagtcaca
    21001 tacactttgg catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg
    21061 cccagacgag gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc
    21121 tgcgctacgc gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa
    21181 ggggctgtta gccccttttt aaaaattaat ttcaggtgga agggctgttc acgttggcct
    21241 gataagacgc gccagcgtca catcaggcaa tccatgccgg atgcggcgta aacgccttat
    21301 cccgcatgga accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata
    21361 cttcgatcac atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca
    21421 taccgttacg gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt
    21481 catagataac cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt
    21541 cggtaaccat acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt
    21601 ccgccagacc gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt
    21661 tcacttcgtc gatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt
    21721 caatcacttt acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg
    21781 acgccgcagc caaggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct
    21841 tcactttaac ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tcgacagaac
    21901 cctgtacgtc tgccttcagg acgatattca cttcgtgaac ttcgccttcg gtcatgttgg
    21961 cgaacatgtt ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac
    22021 cctgacgata gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat
    22081 cacccgcagc cggaacaccg gacaagccga ggatttccac cggaatggac ggacccgctt
    22141 ccagcacttc ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac
    22201 acagaacgat atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac
    22261 cacgaccttt atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg
    22321 ctttcagctc cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgataccgg
    22381 tacccgcttt cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga
    22441 tgccgtactg ggagagttcg ttcttaacgc gatccggatc ggcttctggt ttatcgatct
    22501 tgttcactgc aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg
    22561 tctgcggcat tacgccgtcg tcggcagcaa caaccaggac tacgatgtca gttgcctgcg
    22621 caccacgtgc acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca
    22681 tgccgttttc agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc
    22741 cagaggccac tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt
    22801 gacccatgat ggtcacaacc ggagcgcgcg gttcagccgc agctcccgtg tcacggtcgc
    22861 tcattaccgc ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt
    22921 cagcaaccag ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca
    22981 gtttcatcat cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc
    23041 caacggtgat agtttcgccg atcacaacgt cacggttaac ggcctgagca ggcttctgga
    23101 agccttgctg cagcgaagaa cctttacgtt ttccgccctt accgccacgt actgctgcgc
    23161 gtgcttcttc acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg
    23221 ctttcgcgtt acgaccacgg ccacggccgc cttcgacttc acgatcgctt tcgtcttctg
    23281 cctggcgagc atgttgagaa gtagtgacgt gataatcgct ggaatcctca gtcggttccg
    23341 cgttatcagt ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg
    23401 cttcttcttc gagtttacga cgcgcttctt cttcggcttt acgcttgagc tctgcagctt
    23461 cctgctcacg gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc
    23521 tcactttgtc tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt
    23581 gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct
    23641 cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag
    23701 cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt
    23761 tgctttttcc accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca
    23821 atttgtccgg gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg
    23881 cagacacaga gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc
    23941 gttccacgga ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt
    24001 tccttcctgc tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca
    24061 taatcagtgc tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc
    24121 cctgttcggc gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat
    24181 cacgatctac cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt
    24241 cttcctgggc ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa
    24301 cggtcggctc atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt
    24361 cttccagcgt cgagaagcct tcctctacca gaacagtcgc gaagtcttcg tcgatgtcga
    24421 gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt
    24481 cgtcaacggt catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct
    24541 gaccgttacg gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt
    24601 gtttatcttc atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa
    24661 actgcgccgg gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag
    24721 aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt
    24781 tatcgttggt tttcaccgcg attttcgcac gagaacccgg gtcgcgagcc gctgctttaa
    24841 tttcaatcac ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt
    24901 ccggcttgga acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataaa
    24961 gcacgccacg aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca
    25021 ggatcacggc ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt
    25081 tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac
    25141 gttcggcttc acgcactttc tgcacgataa cctgttttgc cgtctgggtg gtgatacggt
    25201 caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt
    25261 cataacgtgc ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta
    25321 accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga
    25381 tctcttgttc atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa
    25441 tcttctcgcg aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt
    25501 tgttcatcgc gggcttttca cctcatccag actattaaaa gtggggaacc aagttcgcct
    25561 tctggatatt actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt
    25621 caccgtctac cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga
    25681 gaaccagagt cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc
    25741 gatcgagacc cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat
    25801 ccagcacagc acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt
    25861 cactatcaat atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca
    25921 gttcaaaacc cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg
    25981 tggacaagcc cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca
    26041 gataacaaaa aaccccgata aatcggggct ttatataact gaaccctata accgcaactg
    26101 cggtctggag cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc
    26161 acagtatatt tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga
    26221 cgaccttcgg gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt
    26281 ggcaaattct actcgttttg ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag
    26341 acgggacgta aaatctgcag gcattatagt gatccacgcc acattttgtc aacgtttatt
    26401 gctaatcacg tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc
    26461 tgctctccct taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata
    26521 aaagatgatt aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc
    26581 aggctcatca tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct
    26641 cccggtaggt caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact
    26701 gctgtggatg cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc
    26761 agacgaagag gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc
    26821 acgtctgatc gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg
    26881 cgcatttcat aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc
    26941 cgtgactggt accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga
    27001 cggtagcacc tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa
    27061 tgctgaactg cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg
    27121 tcgtcatgaa atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga
    27181 aaaagcttac tccacggact ccaacatgct tggtgcaacg catgaagcga aggatctgga
    27241 atacctcaac tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga
    27301 gagcgtgaag atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc
    27361 gttgaacggt aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg
    27421 cggtcgtcac ggcctgggca tgagcgacca gattgaaaac cgtatcatcg aagcgaaaag
    27481 ccgtggtatt tacgaagctc cggggatggc actgctgcac attgcgtatg aacgcctgtt
    27541 gaccggtatt cacaacgaag acaccattga gcagtatcac gcgcatggcc gtcagttggg
    27601 ccgtctgctg taccaggggc gttggtttga ttcccaggcg ctgatgctgc gtgactctct
    27661 gcaacgctgg gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa
    27721 cgattattca atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac
    27781 gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt atcggtcaac tgaccatgcg
    27841 caacctggat atcaccgata cccgcgagaa acttttcggt tatgccaaaa ctggcctgct
    27901 ttcctcctct gccgcttcag gcgtgccgca gatggagaat ctggaaaaca aaggccagta
    27961 attcgacatc aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc
    28021 tttctgagca tcacataatc gaaaacatca tggtttgcat ccacaaccag tgagtctcct
    28081 ttatagtgta caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc
    28141 cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga
    28201 tccacaccat ccccaagaac ggggctatac cagataaaca tcggaacatg atatgcctgc
    28261 tggctggcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg
    28321 ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga
    28381 cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc
    28441 ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt
    28501 aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc
    28561 ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcacg catggcgata
    28621 ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga
    28681 aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat gtcatggctc
    28741 aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcaccgctt
    28801 attgcttgat taaacagttt gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag
    28861 cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg
    28921 taggtatcaa tacctgtatc cctgactgat aattgaaaat acggcactgt gtttgcaatt
    28981 gagagtaatc tttgatgctc tttcgctgct aaagcaaaat aattgagatt gaaaaacggc
    29041 gtataggtag caaatcgcga cgctagtata tatggactga acgcattttt atttttggca
    29101 tctttataag caaattgaca agcggaaaat aaacttcccg aaatgacaat cagcaataat
    29161 attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac agccaaaaaa
    29221 agaagggata aaaaggcaaa agcacataga taagggctat acattcccag cattttggca
    29281 acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca
    29341 aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt
    29401 gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcaga
    29461 acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata
    29521 ccaaaaacaa taacactaag atacaacaac caatgagatt taaaacttct agcaaatttg
    29581 ttgaatactg tcatccagaa atcc
//
LOCUS       DAZFKY010000042        28923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFKY010000042 DAZFKY010000000
VERSION     DAZFKY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            97..1050
                     /gene="ybhD"
                     /locus_tag="ACHKCL_001648"
     CDS             97..1050
                     /gene="ybhD"
                     /locus_tag="ACHKCL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5762643.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1091..2086)
                     /gene="pgl"
                     /locus_tag="ACHKCL_001649"
     CDS             complement(1091..2086)
                     /gene="pgl"
                     /locus_tag="ACHKCL_001649"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5762644.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2241..3059
                     /gene="ybhA"
                     /locus_tag="ACHKCL_001650"
     CDS             2241..3059
                     /gene="ybhA"
                     /locus_tag="ACHKCL_001650"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5762645.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3060..4118)
                     /gene="modC"
                     /locus_tag="ACHKCL_001651"
     CDS             complement(3060..4118)
                     /gene="modC"
                     /locus_tag="ACHKCL_001651"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5762646.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4121..4810)
                     /gene="modB"
                     /locus_tag="ACHKCL_001652"
     CDS             complement(4121..4810)
                     /gene="modB"
                     /locus_tag="ACHKCL_001652"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5762647.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4810..5583)
                     /gene="modA"
                     /locus_tag="ACHKCL_001653"
     CDS             complement(4810..5583)
                     /gene="modA"
                     /locus_tag="ACHKCL_001653"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5762648.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5750..5899)
                     /gene="acrZ"
                     /locus_tag="ACHKCL_001654"
     CDS             complement(5750..5899)
                     /gene="acrZ"
                     /locus_tag="ACHKCL_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5762649.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6028..6816
                     /gene="modE"
                     /locus_tag="ACHKCL_001655"
     CDS             6028..6816
                     /gene="modE"
                     /locus_tag="ACHKCL_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5762650.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6884..8356
                     /gene="modF"
                     /locus_tag="ACHKCL_001656"
     CDS             6884..8356
                     /gene="modF"
                     /locus_tag="ACHKCL_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5762651.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            8618..9634
                     /gene="galE"
                     /locus_tag="ACHKCL_001657"
     CDS             8618..9634
                     /gene="galE"
                     /locus_tag="ACHKCL_001657"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5762652.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9644..10690
                     /gene="galT"
                     /locus_tag="ACHKCL_001658"
     CDS             9644..10690
                     /gene="galT"
                     /locus_tag="ACHKCL_001658"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5762653.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10694..11842
                     /gene="galK"
                     /locus_tag="ACHKCL_001659"
     CDS             10694..11842
                     /gene="galK"
                     /locus_tag="ACHKCL_001659"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5762654.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11836..12876
                     /gene="galM"
                     /locus_tag="ACHKCL_001660"
     CDS             11836..12876
                     /gene="galM"
                     /locus_tag="ACHKCL_001660"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5762655.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            13078..13830
                     /gene="gpmA"
                     /locus_tag="ACHKCL_001661"
     CDS             13078..13830
                     /gene="gpmA"
                     /locus_tag="ACHKCL_001661"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5762656.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13996..15048)
                     /gene="aroG"
                     /locus_tag="ACHKCL_001662"
     CDS             complement(13996..15048)
                     /gene="aroG"
                     /locus_tag="ACHKCL_001662"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5762657.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15364..15744
                     /gene="ybgS"
                     /locus_tag="ACHKCL_001663"
     CDS             15364..15744
                     /gene="ybgS"
                     /locus_tag="ACHKCL_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5762658.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15858..16799
                     /gene="zitB"
                     /locus_tag="ACHKCL_001664"
     CDS             15858..16799
                     /gene="zitB"
                     /locus_tag="ACHKCL_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5762659.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16796..17515)
                     /gene="pnuC"
                     /locus_tag="ACHKCL_001665"
     CDS             complement(16796..17515)
                     /gene="pnuC"
                     /locus_tag="ACHKCL_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5762660.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17553..18596)
                     /gene="nadA"
                     /locus_tag="ACHKCL_001666"
     CDS             complement(17553..18596)
                     /gene="nadA"
                     /locus_tag="ACHKCL_001666"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5762661.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18874..18949)
                     /locus_tag="ACHKCL_001667"
     tRNA            complement(18874..18949)
                     /locus_tag="ACHKCL_001667"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18914..18916),aa:Lys,seq:ttt)
     gene            complement(19096..19171)
                     /locus_tag="ACHKCL_001668"
     tRNA            complement(19096..19171)
                     /locus_tag="ACHKCL_001668"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19136..19138),aa:Lys,seq:ttt)
     gene            complement(19175..19250)
                     /locus_tag="ACHKCL_001669"
     tRNA            complement(19175..19250)
                     /locus_tag="ACHKCL_001669"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19215..19217),aa:Val,seq:tac)
     gene            complement(19302..19377)
                     /locus_tag="ACHKCL_001670"
     tRNA            complement(19302..19377)
                     /locus_tag="ACHKCL_001670"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19342..19344),aa:Lys,seq:ttt)
     gene            complement(19380..19455)
                     /locus_tag="ACHKCL_001671"
     tRNA            complement(19380..19455)
                     /locus_tag="ACHKCL_001671"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19420..19422),aa:Val,seq:tac)
     gene            complement(19491..19566)
                     /locus_tag="ACHKCL_001672"
     tRNA            complement(19491..19566)
                     /locus_tag="ACHKCL_001672"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19531..19533),aa:Lys,seq:ttt)
     gene            complement(19731..20522)
                     /gene="cpoB"
                     /locus_tag="ACHKCL_001673"
     CDS             complement(19731..20522)
                     /gene="cpoB"
                     /locus_tag="ACHKCL_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5762662.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20532..21053)
                     /gene="pal"
                     /locus_tag="ACHKCL_001674"
     CDS             complement(20532..21053)
                     /gene="pal"
                     /locus_tag="ACHKCL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5762663.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21088..22380)
                     /gene="tolB"
                     /locus_tag="ACHKCL_001675"
     CDS             complement(21088..22380)
                     /gene="tolB"
                     /locus_tag="ACHKCL_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5762664.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22513..23697)
                     /gene="tolA"
                     /locus_tag="ACHKCL_001676"
     CDS             complement(22513..23697)
                     /gene="tolA"
                     /locus_tag="ACHKCL_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5762665.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSS
                     GKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGK
                     TCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDF
                     KP"
     gene            complement(23762..24190)
                     /gene="tolR"
                     /locus_tag="ACHKCL_001677"
     CDS             complement(23762..24190)
                     /gene="tolR"
                     /locus_tag="ACHKCL_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5762666.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24194..24886)
                     /gene="tolQ"
                     /locus_tag="ACHKCL_001678"
     CDS             complement(24194..24886)
                     /gene="tolQ"
                     /locus_tag="ACHKCL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5762667.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(24883..25287)
                     /gene="ybgC"
                     /locus_tag="ACHKCL_001679"
     CDS             complement(24883..25287)
                     /gene="ybgC"
                     /locus_tag="ACHKCL_001679"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5762668.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25437..25730)
                     /gene="ybgE"
                     /locus_tag="ACHKCL_001680"
     CDS             complement(25437..25730)
                     /gene="ybgE"
                     /locus_tag="ACHKCL_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5762669.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25730..25843)
                     /gene="cydX"
                     /locus_tag="ACHKCL_001681"
     CDS             complement(25730..25843)
                     /gene="cydX"
                     /locus_tag="ACHKCL_001681"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5762670.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(25858..26997)
                     /gene="cydB"
                     /locus_tag="ACHKCL_001682"
     CDS             complement(25858..26997)
                     /gene="cydB"
                     /locus_tag="ACHKCL_001682"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5762671.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27013..28581)
                     /gene="cydA"
                     /locus_tag="ACHKCL_001683"
     CDS             complement(27013..28581)
                     /gene="cydA"
                     /locus_tag="ACHKCL_001683"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5762672.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7245 a         7638 c         7100 g         6940 t
ORIGIN      
        1 aataacattt tttcattaat ttaagttggc taaatgcagt taaaattttt aacggccagc
       61 cacccaaaat cgtcgcttcc atttcccgga gtaataatga agcatgaatt atcaagtatg
      121 aaggcatttg tcatactggc agagtccagt tcatttaaca atgccgctaa attactcaat
      181 attacgcaac ctgcattaac acgaagaata aaaaagatgg aggaagattt acatattcaa
      241 ctatttgagc gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctccca
      301 gaggcgcggg aattaataaa gaaattcgat gaaacgcttt ttaatattcg tgatatgaat
      361 gcttatcatc gtggtatggt gacgttagcg tgtattccga ccgcagtatt ttatttttta
      421 ccgctggcaa tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa
      481 caaggcacga ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac
      541 atgaacaacg tcacaaattc atccattgat tttaccccac tggtcaacga gccgttcgtg
      601 ctggcctgtc ggcgtgacca tccattggct aaaaagcagc ttgtagaatg gcaggagtta
      661 gtgggttaca aaatgattgg cgtacgttcc tccagtggca accgattgtt gatagaacaa
      721 cagctagccg acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg
      781 tcattaggac tggtagaggc gggactggga atttcggcgc ttcctggtct ggcaatgccc
      841 catgcgccct attcttctat tattggtatc ccactggtgg aacccgttat acgtcggaca
      901 ttggggatta ttcgccgcaa agatgccgtg ctttctccgg cagcagaacg cttttttgcc
      961 ttgctgatta atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc
     1021 cagagacatg cgttacagga gataggttaa tacaccggcg caggagagac tgcgccgggt
     1081 aaatcagcgg ttagtgtgcg ttaaccacca cccacattgg tccctgcccg accgcgtagc
     1141 ggcctttttc atgcagtagc ccctgctcac caacaatttc gtataccgag atgtggtgcg
     1201 atttttgccc ggcggcaatc agatacttac cacggtgatc gatattaaag ccgcgcggct
     1261 gggtttcagt tggctggaag ccctctttac tcaacacgct gccatcttcc gaaacgctga
     1321 agacagtaat caggctggcg gtacggtcac aggcatataa gtgacgacca tccggggtaa
     1381 tatgaatatc agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct
     1441 ggacacattc gatattgccg tgcggatctt tcagttccca gacatccact gagctgttta
     1501 actcattgac gcaatatgca tattgttcgt ttggatggaa taccatatga cgcgggccgg
     1561 ccccttcaac ggtagtcact tccgcagggt cctgcgccac cagatgacca tcatcgctga
     1621 ccgtaaacag gcaaatgcga tcctgcttta atgccggaac ccacagcgta cggttgtccg
     1681 gtgagatatt ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgccaactg
     1741 gcaggccatc ttccagacgc gttacgctca cgttacccgc attgtaagaa cctacaaaga
     1801 caaactgccc ctggtgatcg gtggaaatat gcgtcggact acccggcagc gcagactctg
     1861 cggcaaaggt cagtgcgcca tcgtccgggg cgatacgata cgccaggacg cgaaactcag
     1921 ggcgaacacc aacatagaga taacgtttgt ccgggctgac caccatcggc tgcacctgcc
     1981 ccggcacatc gacaacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt
     2041 gaatttgctg gctctcaggg ctggcgatat aaactgtttg cttcatgaat gctcctttgc
     2101 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
     2161 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagaag aaaattctta
     2221 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
     2281 tattgacccc gaaaaagacc ctgctacctt catcgataga agccctggcc cgcgctcgcg
     2341 aagcaggtta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
     2401 atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt
     2461 atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctacaactca
     2521 ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgac gagatggttt
     2581 atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg
     2641 aacagcgtcc gactttcaca caagtcgctt ctctggctga aacggcgcaa caagttaacg
     2701 ccgtatggaa gttcgccctc acgcacgatg acctgccgca attgcagcat tttggtaagc
     2761 atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg
     2821 cacgcggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt
     2881 cgatggaaaa cgtcgtggcg ttcggcgata actttaatga tatcagtatg ctggaggccg
     2941 ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg
     3001 tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat
     3061 caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcgcc
     3121 agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc gacttccagt
     3181 tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta
     3241 cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg
     3301 atacgtagcg cagcttgcag cggttcgtcc agcttattga cccacaaatg ctgatcgccc
     3361 agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg
     3421 ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactacccca gacttcttcc
     3481 agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg
     3541 aggatctcat ccagcgaatg gctgacatac aacatcggga tgttgatttc ccgcgtcaga
     3601 cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca
     3661 tccagcaaca gcaattctgg tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt
     3721 tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc
     3781 accagcttat cgaactgatc gaccatgctt ttcgccatgc cgtagcgcag attgccacgc
     3841 actttgtaat gcgggaacag ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc
     3901 ggcgtcaggc agataccttt ttcggcatca tttagtaccc gaccattgag gacaatcctc
     3961 cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg
     4021 gagacgccaa agatagcagt gatgccatta gcgggcagcg tttcattaat agtcaggcaa
     4081 tggttgccca acgtctggga aaaattcagt tccagcatga ttagcgcccc gcccgttcac
     4141 ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa
     4201 taatgcacag tctcgctgcg ccactttccc cgccgggggt ctgaatcagg gtatacatgg
     4261 cagaaggaat ggttcgcgtt tcaccaggaa tgttcgacac aaaggtgatg gttgcgccaa
     4321 attcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca
     4381 gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct
     4441 gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa
     4501 acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa
     4561 accagtcata cagacgttca ccgataaatc cgcgccgccc catcgaaact aataataagt
     4621 aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt
     4681 tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa
     4741 acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg
     4801 tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg cctgcggtcc
     4861 cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca caacagcaac
     4921 cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt
     4981 gctagcaact gcgtcagaac cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa
     5041 cgccagcgcg ccacgaacat cttccgccgg agccagtttc ggggagagcg tatcccatgc
     5101 gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt ccggatcgcc
     5161 aaccgccagg cgaccgccat tcagcagtga agtccagttg gttttgctgt cgatggtaaa
     5221 atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg
     5281 acgcgtagct gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc
     5341 agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag tagatgacga
     5401 agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag caatgtcctg
     5461 cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc
     5521 attgccagca acagcgaaag agagtgccgc cccagcaaac aagttcaacc atttacgagc
     5581 cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta actcggaatt
     5641 ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg gcaggaaaag
     5701 caggaagttg agaaaaagaa aatgcccgac taagcgggca ttcagggaat caatgatttt
     5761 gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt
     5821 aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg aataccagac
     5881 tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta
     5941 agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca
     6001 caataagact tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc
     6061 caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat tgcgctttcc
     6121 ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc
     6181 attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa
     6241 ggtggcggcg gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta
     6301 ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg
     6361 aacagcctgc tggccgcgat ctcacgtttt tcactccaaa ccagcgcccg taaccagtgg
     6421 ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg
     6481 gctgatggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc gcgtctgggg
     6541 ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg cattactcag
     6601 gacgaagcgg tcgcgcaaaa cgctgacaac caattaccgg gtattattag tcatattgag
     6661 cgcggcgcag agcagtgcga agtattaatg gcgctaccgg acgggcaaac actgtgcgcc
     6721 acagtgccgg taaatgaagc gacttctctt cagcaaggac agaatgtcac ggcctacttt
     6781 aatgccgaca gcgtgattat cgccacactg tgctaagcgt gttgacaatt tgttatgaaa
     6841 cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat
     6901 tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt
     6961 aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agcgggaaat cggccctggc
     7021 ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca
     7081 catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat ggcagcggaa
     7141 taacaccgat atgctcggcc ctggcgaaga tgacaccgga cgcactacgg ctgagattat
     7201 tcaggatgaa gtaaaggatg caccgcgctg catgcaactg gcacagcagt tcggtattac
     7261 ccccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct
     7321 gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct
     7381 ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat
     7441 tactctggta ctggtgctca atcgcttcga tgagatcccg gagtttgtcc agtttgctgg
     7501 cgtgctggcg gattgcacgt tagcggaaac tggcgctaaa gaggaactgc tccaacaagc
     7561 actcgtcgcg caactggcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga
     7621 tgaaccttca gcacgtcacg ccttacccgc caacgaaccg cgcattgtgc tgaataatgg
     7681 cgtggtttct tataacgatc gccccattct taataacctt agctggcagg tgaatccagg
     7741 tgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat taagcctgat
     7801 tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggac gacgtcgtgg
     7861 cagcggcgaa accatctggg atatcaaaaa gcatatcggt tacgtcagca gtagtttgca
     7921 tctggattac cgggtcagta ctactgtgcg taatgtgatt ctttctggct attttgattc
     7981 gattggcatt tatcaggccg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga
     8041 tattctcggc attgataaac gcacggctga cgctccgttc catagtcttt cctggggaca
     8101 gcagcgtctg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc ttattctcga
     8161 cgaaccattg caggggcttg atccgctcaa tcgccagctt attcgccgtt ttgttgatgt
     8221 gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccatgctg aagatgcgcc
     8281 ggcttgtatt acccatcgtc tggagttcgt gccggacggt ggcctctatc attatgcgct
     8341 gacaaaaata aactaagtcg gtaatgctga ctgtgccgga tgcggcgtaa acgctttatc
     8401 cggcacacgg tcagacgcat ccaggcatga aactgcgtct ttttttcaga taaaaagcgc
     8461 aatcattcac aatccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt
     8521 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
     8581 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg tcactggtgg
     8641 tagcggttac attggaagtc atacctgtgt gcaattactg caaaacggtc atgatgtcat
     8701 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
     8761 cggcaaacat ccgacgtttg ttgaaggcga tatccgtaac gaagcgttga tgaccgagat
     8821 cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgttggcga
     8881 atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggtactc tgcgcctgat
     8941 tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta
     9001 tggcgatcag cccaaaattc catacgttga aagcttcccg accggcacac cgcaaagccc
     9061 ttacggcaaa agcaagctga tggtggaaca gatcctcacc gatctgcaaa aagcccagcc
     9121 ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcacatc cgtcgggcga
     9181 tatgggcgaa gatccgcaag gcattccgaa taaccttatg ccatacatcg cccaggttgc
     9241 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagacggtac
     9301 tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
     9361 aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag
     9421 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt
     9481 tgcaccgcgt cgcgagggcg accttccggc ctactgggcg gacgccagca aagccgaccg
     9541 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
     9601 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
     9661 cgttgatcat ccacatcgcc gctacaacca gctcaccggg caatggattc tggtttcacc
     9721 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
     9781 tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
     9841 ccccgattac accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac
     9901 gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag
     9961 ccgggtgatc tgcttttcac cggatcacag taaaacgctg ccagaactga gcgttgcggc
    10021 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gaactgggga aaacataccc
    10081 gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca
    10141 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag accgcctgca
    10201 aaaagaatat tttgccgagc agaaatcgcc aatgctggtg gattatgttc agcgcgagct
    10261 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagcagttg tgccttactg
    10321 ggctgcctgg ccgttcgaaa cgctacttct gcccaaagcc cacgttttgc ggatcaccga
    10381 tttgaccgat gcccagcgca gcgatttggc actggcgttg aaaaagctga ccagtcgtta
    10441 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg caccgtttaa
    10501 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgttgcgctc
    10561 cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagaaaccc agcgagacct
    10621 gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
    10681 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
    10741 ctaccctgcc actcatacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
    10801 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg
    10861 tgcgccacgc gatgaccgta aagttcgcgt aatggcagcc gattatgaaa atcagcttga
    10921 tgagttttcc cttgatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt
    10981 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt
    11041 gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc
    11101 ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct
    11161 taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct
    11221 aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgtcgct cactggggac
    11281 caaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg
    11341 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt
    11401 cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga
    11461 gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
    11521 tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga
    11581 gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt
    11641 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
    11701 tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt
    11761 cgcagaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
    11821 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
    11881 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg
    11941 actttacttt cagcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc
    12001 tgtgccagcc cggaatgcta tcaggatcag gccgcgtttc tgggtgcctc tattggtcgt
    12061 tatgccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt gacgctttcg
    12121 ccaagtcagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg
    12181 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    12241 cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt
    12301 atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    12361 gtctatttca atcttgacgg caagcagtct gacgtgcgca atcacaagtt gcagattctg
    12421 gcggacgaat atctgccggt tgatgaaggc ggtattccgc acgacggcct gaaatctgtc
    12481 gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac
    12541 gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat
    12601 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    12661 accaccgctc cggctctgca attctactcc ggcaactacc tcggcggcac accgtcgcgg
    12721 ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac
    12781 agcccgaacc accctgaatg gccgcaaccg gactgtttcc tgcatcctgg tgaagagtat
    12841 tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct
    12901 ttatctcata ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca
    12961 acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc
    13021 gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag taagtatatg
    13081 gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt
    13141 ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca
    13201 ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacctc tgtgctgaaa
    13261 cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt
    13321 gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg
    13381 gaaactgctg aaaaatatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg
    13441 actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg
    13501 aaactgagcg agaaagaact gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg
    13561 atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc
    13621 gctgcacacg gtaactcgtt acgtgcgctg gtgaaatatc tcgataacat gagcgaagaa
    13681 gagattcttg agctgaatat cccgaccggc gtgccgctgg tttatgagtt tgacgagaac
    13741 ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca
    13801 gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaatg agaaagccga
    13861 ctgcaagtga gtcggctttt ttgtttgtcg tcgctaacaa tggagcacat tgcctgatgc
    13921 gacgctgcgc gtcttgtcag ggctacggtg attcatcgga tacgccactc tgacggcgca
    13981 tccgacaatt aaactttacc cgcgacgcgc tttcactgcg ttcgccagtt gacgtaacag
    14041 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccat aggccagcgg
    14101 ctccccgctc tccaggctct gattgccttc caccagatga ctttccacca tcacaccgat
    14161 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    14221 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc
    14281 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    14341 accgccgcgc agaatgatat ggcaatcgcc gttaccgctg gtattcacaa tcgccgaatg
    14401 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    14461 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    14521 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    14581 caggtcagcg agatactgag gagtgatcat atcgaggaat tcacccgccg ctggcagacc
    14641 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    14701 gctgttatcc atatgcggat cgttaatcag ccctttccag cccaccgtag tacgcggctt
    14761 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    14821 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    14881 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    14941 aaccgtattc gcggcatttt cagtagcggg gaatttttcc agcaatgcga caggaggaag
    15001 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    15061 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    15121 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    15181 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    15241 atttcaagat tctctgctgg atttctttgc ctggctgaaa tcttaaacca atgccttata
    15301 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    15361 cctatgaaaa tgacaaaact ggccacacta tttctgactg ccactctaag ccttgccagc
    15421 ggtgccgcac tggctgccga tagcggagcg caaaccaata acggccaggc aaacgccgca
    15481 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    15541 aataacgggg taaataccgg ttctggcggc actatgctgc atccggatgg ttcttcaatg
    15601 aacaatgacg gcatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcgc
    15661 tgcccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    15721 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    15781 ctccctttta atttttccct tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    15841 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    15901 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    15961 aagtcattgg tggttttctt tccggttctc tggctttgct ggccgatgcg ggtcatatgt
    16021 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    16081 ccaccattcg ccacactttc ggttggctaa gattgaccac gctcgcggcg tttgtgaatg
    16141 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    16201 cgccgcgtcc ggtcgagggc ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    16261 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    16321 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    16381 tgattattat ctggaccggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    16441 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgagg
    16501 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    16561 aagtccgcaa tgtgcaccat gtgcatgtat ggatggtagg cgagaagccg gtgatgacgt
    16621 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    16681 acctgatgga tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc
    16741 atgggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    16801 gggacagcgc gcgtgagcct ctttcgcgtg cgctattgat ccacatccgg ctgccgttga
    16861 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    16921 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    16981 gagtcatcag aatcattgcc acgatagata acaccatcat gcaagaatcc cagaacggga
    17041 aagcgtccgg ttgtagttca ggcatcgcaa cctgtaatcc taatgcttgc atgatgatga
    17101 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    17161 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttta
    17221 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    17281 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    17341 caaacaaggt gacgttgatc aagccaaaga agtagttgct gatcttctcc agactggcaa
    17401 ggccaataca cagcaacccg gcgattgtgc ctaccgcttc gatccatgag agatcataac
    17461 cgcctgctcc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    17521 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    17581 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tttcatcaac atgaacctcg
    17641 taattgcttc cttcctgttc taatgcctca gcgatggcct ggaggccatt catggccatc
    17701 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    17761 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    17821 ataagcctct gatgtggcaa tgttttcgca gcagcgatca gttgactggt ggaaccgacc
    17881 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    17941 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa actcatcatg cacaatgcag
    18001 gcaccctgcc agcataaaat gtctgcgccc gtcttttttt gcacgtaacg ccccagatgt
    18061 ttgtcgggtg cccagatgat tttttcaccc aaactatcca gatgatcaat aagttcgacg
    18121 gcaatgcttg aagttaccac ccaatctgcc cgcgctttta ccgcagcaga agtgttggcg
    18181 tagacgacga cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg
    18241 cagccgagat ccagtgaaca ctcagcctga agtgtcggca tcagaattgt tttttccgga
    18301 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa cgtagaagcg
    18361 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    18421 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    18481 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    18541 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacatcac gctcatctta
    18601 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtgatattc gctgttatcg
    18661 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    18721 tacgtatgag atgccgaatg ccacactttg cgtcttattc ctccgacgta aagttgttta
    18781 cgcagcaagc gcggagtatg tcatcagggt caagaattga aattgagaga tgcctgaata
    18841 gcaaaaaagc gcctttaggg cgctttttta cattggtggg tcgtgcagga ttcgaacctg
    18901 cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgaccca tacgggtgtt
    18961 acctgaaaga tttctactcg gaaccaatct ttaaaattgg tgggtcgtgc aggatgactc
    19021 gcttcgcttg ccctacgggc cgttgctgac gcaacgttat ccttcacgct taacatctga
    19081 gtttgatgtt aaaattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    19141 tcaactgctc taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg
    19201 ccgcccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc
    19261 tggatactac aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatt
    19321 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccggt
    19381 ggtgggtgat gacgggatcg aaccgccgcc cccctccttg taagggaggt gctctcccag
    19441 ctgagctaat cacccgatat tacgctggat actacaacta actaggttag tggtgggtcg
    19501 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    19561 cgacccactt tttcgttgct ttcggtttgt ttgatatccc gtggcaacgg cggcatatat
    19621 tactgatttc agacttgagc gcaacaaaaa tttcgatgta gatcactcaa ctgcttatga
    19681 ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg
    19741 ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag ggtatttact gataacctgc
    19801 tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct gcatgatgac gccgacttta
    19861 aacatcgcat ctgcagcctt tggtgacttc ggatagtttt tcactaccga agcaaaatag
    19921 tacgccgcat catctttttt acccttgttg tagtttaact gacccagcca ataattggcg
    19981 tttggcaggt aagttgaatc agggtaattt ttgatgaaat tctgaaatgc caccattgcg
    20041 tcatcctggc gggatttatc ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca
    20101 tcaccgcttt tcaccggcgc gccagcattc gcagtaccag catcagctgt cggcgtcgtt
    20161 gatgccgccg caccgctttg atcaccgctg gttgattgcg ccgctgcacc accgctgctg
    20221 aggctgtcga tctgcaacag gatctgcttt tgccgctcca cgacctgatt cagttgatac
    20281 tgattttcct gaatctgacc acgcagggaa tcaatatcgg attgattatc agagagttgt
    20341 tgctggagtt gggttaaaag ctggctgtga gcattagaaa tacgctcaag ttgaatgacg
    20401 cggtcttcga ccgagcctga gccgacacta ctgattggtg cctgagcaaa agcggcccag
    20461 ggggccgcta taccaaccag taacgacaga ctcaatagtt gatgtctgaa gttactgctc
    20521 atgcaattct cttagtaaac cagtaccgca cgacggtttt tggagtatgc cgcttcgtca
    20581 tgacccagta ctgcaggttt ttctttaccg taagaaacga tggagatctg gtctgcagaa
    20641 acgcctttac cctgcaggta catcttaacg gcgttcgcac gacgttcacc cagggagatg
    20701 ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg
    20761 ttgctacgca ggaagtttgc atgtgcatcc agcatttgag cgaagtcaga acggatatcg
    20821 tacttgtcca gatcgaagta aacgatgttg ttctgctgca gctgttgcat ttgcagacga
    20881 gcctgctctt cggaagacat gttgccgttg ccgccgttcg catccatacc agtgccggca
    20941 cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt
    21001 gccataacag gcagagcaat catcagccct ttcagcactt tgttcagttg catttcaatg
    21061 attcctttac tattcaatta attattatca cagatacggc gaccaggcag ggaatttgac
    21121 ctgtccatca gttgccggaa gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag
    21181 cacggatccc atcccctgag aagagctgta gattaccata gtgccgttag gtgccagact
    21241 tggcgtttca tccaggaacg tggacgacag aacttgtacg cctcccgttg ccagatcttg
    21301 tttggcaatg tgctgctgcc caccattgga gctgaccatt accataaatt taccgtcgct
    21361 gctgacatcc gcatcctggt tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc
    21421 gttgatattc actttataaa cctgtggacg accggcctgg tcagaagtaa atgccaggtt
    21481 ctggctgtcc gggaaccagg tcggttcggt attgttactg cgaccatcag tcacctggcg
    21541 gatctgacca gaagccaaat ccattacgta caggttcagg ctaccggttt tcgacaaggc
    21601 gaatgccagt ttgctgccgt ctggcgagaa tgcaggcgca ccgttgtgac gcgggaatga
    21661 agccacctga cgtacagcgc cgtttgccag cgtttgtata accagcgcgg aacgaccgct
    21721 ttcgaaggtc acataagcca gtttagaacc gtctggtgac cacgccgggg acatcagcgg
    21781 ctgtggtgaa cggtgaacga caaactggtt gtaaccgtca tagtcagata cgcgcagttc
    21841 atacgggaac tgaccgccgt tggtctgaac aacgtaggca atacgggtac ggaacgcacc
    21901 tttaataccg gtcagttttt caaacacttc atcactggcg gtatgaccag cataacgcag
    21961 ccactgcttg ttcactttgt acgagttctg agcaagtaca gtacccggtg cgccgccagt
    22021 gtcaacaagt tgataagcaa cattgtaaga accatccgga ttcggagtga cctgaccgac
    22081 aactacagcg tcaataccca gcgcggacca tgcagctggt tgtacttcct gcgcactacc
    22141 cggctgctgt ggcagacgag cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc
    22201 ggcagcaacg atgccgccaa tatcttcagg tgccgcacca gggcccgccc actggaaagg
    22261 aacaactcca ataggacgac cggaatctac accgctgtcg atcacaatgc ggacttcagc
    22321 atgcagaact gatgcccaca gtatgagaaa accaaatgct actcgtaatg cctgcttcat
    22381 catatctccc ttatctggac cgatggccca cgataattta gcagaatgtt aacaaactaa
    22441 aatacacaaa actaccagaa ccccgtggca accggtgcct gatgttgacc gtcggaacag
    22501 tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt ttgaacactt catatactgc
    22561 ctggcttggt ggtttcggga tcttcgcaag tttagctgct gccaacgcag cctgacaaag
    22621 tgcgggatcg ccaccttcag gtttgatatc cagtaacata ccatcgggtg ccagttttat
    22681 gcgcagcgta caggttttgc ctgcatagga cgatgcgtca tagaacttac tttcgatagc
    22741 agatttaatc tgcccggcat agttattgat atcggcccct gatgcgccat tgtttttagt
    22801 attaccactc ccggcaggcg aagcattgtt ccctttcgcc cctccccccg ttttcggtgc
    22861 attcttacca gagcttagct caccgaaaat atcatctgcc tccgcggcag cttttgctgc
    22921 agcggctttt tcagctgctg cttttgcagc tgccgctttt ttgtctgcgg cggctttttc
    22981 tgccgctgct ttcttatcag ctgctgcctt ttcagcagcc gctttttctg ctgctttttt
    23041 gtcggctgca gctttctctg ctgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    23101 cgcttccgct ttcttcttca gcgccgcagc tgctgcctcg gcttttttct gcgcttcggc
    23161 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    23221 tgcttcttcc gcagctttat catccgcttc ggccttcgct ttagcatctg ccgccgcttt
    23281 cgctgccgcc tcttccgctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc
    23341 ctgctttttc tgttcctgag cagctaaccg ctctttctca agttgcttca ggcgttcctg
    23401 ttcagccgct tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg
    23461 ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac
    23521 cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cagctgaagc
    23581 ttctatattc tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac
    23641 tgctgaaata attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct
    23701 cggtttccaa aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg
    23761 tttagatagg ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca
    23821 gtgctttaat tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt
    23881 tcgggttggc cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct
    23941 ccaggcgatc tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca
    24001 ctggcggatt atcgttactg ctcaccgcct gtgattcagt agcgtctggc aaatcgacct
    24061 ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta
    24121 cgtccagcaa cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg
    24181 ctctggccat ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa
    24241 tcgcggtaaa ctcttccata aagttgtcgt aattcagttc cagtttgttt acacgttggt
    24301 tgagacggtt ataagccatt accgccggaa tagcggcgaa cagaccaatt gccgtcgcaa
    24361 tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga
    24421 gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga
    24481 tggagccaac cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcgcggttca
    24541 tggaaatacg catcgcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg
    24601 cacgatgcag gcgcacaaat tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca
    24661 gattatcccg tttcccctgg ctctcttgat agaggcgaga gagttcgatt ccagaccaga
    24721 atttatcttc aaacgcttcg gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga
    24781 taataatggc ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca
    24841 gaaggctagc cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc
    24901 gacaatagac ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag
    24961 aacctctgct tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa
    25021 agaggtgcca cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc
    25081 aggtgcgtaa tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag
    25141 cgcctgttga ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc
    25201 gacgtaactg gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac
    25261 cggccatcga aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc
    25321 ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg
    25381 agcgtaaaaa tttatgggcc tggcacggcc cataattttt taaagagaat tctgatttaa
    25441 aagaagaaga aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc
    25501 ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg
    25561 gcccacatca gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcgaaacga
    25621 gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc
    25681 agggggcgct tgtccattac cgcatacaag gttgcgataa tcttactcat cagatgtctt
    25741 cttgaccggc tttgcctgat tcgacgtgct caagcgccag cgcggtgatt accccaaacg
    25801 aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta
    25861 gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca
    25921 gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat
    25981 gacgttaagt gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat
    26041 ggtgctggac ggcatcacaa acgggaacat tgcgataccg gcagtcagga tgatgcaggc
    26101 cagcgtcagg gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat
    26161 ggtcagcagc ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt
    26221 gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagcg ggttagaggc
    26281 tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac
    26341 gccagccagt gcgaaacaga ccagtgtcac cagtgccgcc acctgagccg ttgcacgggt
    26401 acgcaggtgc agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat
    26461 catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcagct ggaagaagtt
    26521 accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt
    26581 accgaacgct acaccaatca ccaacggcgg aacgaagcta ccaatgaaga tgccccagtc
    26641 ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg
    26701 gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc
    26761 atagaccatc ggccaggcag caaacagtgc gccgcctgcg gtgatcagcc aaacctggtt
    26821 accgtcccag tgtggtgcga tggagttaat cataattcga cgctcggtgt cgttacgacc
    26881 gaggaaacgg gtgagcatgc ccacccccat gtcgaaacca tcagtgactg caaaaccaat
    26941 cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg
    27001 acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg
    27061 accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc
    27121 caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc
    27181 ggtcagtgac gagttcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg
    27241 gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta
    27301 cagcgcggcg cgcaggagcc attttttctc gccaatgcgg ttgcggatga cactccagaa
    27361 agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcgaa
    27421 gtacagcggc gctacacgcg gaatggagtc tttggttgcc tgttgaatct gcgcttcagt
    27481 cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtccttctt
    27541 catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag
    27601 cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagttcttt
    27661 caggccgata actggagtat ccacggaacg cgttgcaatt atgcccagcg cgtaagggat
    27721 ctggatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca gagtaaaggc
    27781 agcaggcgca ggttgcgttt cccactcagc ttcaatagca gccagtttgg ttttctgcac
    27841 gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa cggcagccat
    27901 accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag
    27961 catataccat gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac
    28021 agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat
    28081 ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt
    28141 cgcaaccaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca
    28201 catatgctga actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga
    28261 ttcgaggaag aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac
    28321 atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc
    28381 cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata
    28441 aatctgtttg ccggaaagga cgtagaccgt ttccataatg gccagcagga acgccatacc
    28501 gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga
    28561 cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga
    28621 accccgttag aaagggtcac acgcatgccc caatacaaaa ttatttgctc gcctctatcg
    28681 ttgccatttc atcaaaagag gaaaagacaa tgataaaaaa gttacaaata tgttaatgaa
    28741 taaaccaaat tgatcccgta attatattac gccgcaaaat ccttacaata aacaggtttt
    28801 tattgagcaa atttgcattt ttcgacagtg atcaatttat agcgaaattg tcacgatctc
    28861 accaatttga tctacgacaa tttatcgtgt tttgcagatt tttttcaaga attaaaaatt
    28921 gat
//
LOCUS       DAZFKY010000043        28853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFKY010000043 DAZFKY010000000
VERSION     DAZFKY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(37..1005)
                     /gene="astE"
                     /locus_tag="ACHKCL_001684"
     CDS             complement(37..1005)
                     /gene="astE"
                     /locus_tag="ACHKCL_001684"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5762673.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(998..2341)
                     /gene="astB"
                     /locus_tag="ACHKCL_001685"
     CDS             complement(998..2341)
                     /gene="astB"
                     /locus_tag="ACHKCL_001685"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5762674.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2338..3816)
                     /gene="astD"
                     /locus_tag="ACHKCL_001686"
     CDS             complement(2338..3816)
                     /gene="astD"
                     /locus_tag="ACHKCL_001686"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5762675.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3813..4847)
                     /gene="astA"
                     /locus_tag="ACHKCL_001687"
     CDS             complement(3813..4847)
                     /gene="astA"
                     /locus_tag="ACHKCL_001687"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5762676.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4844..6064)
                     /gene="astC"
                     /locus_tag="ACHKCL_001688"
     CDS             complement(4844..6064)
                     /gene="astC"
                     /locus_tag="ACHKCL_001688"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5762677.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            6510..7316
                     /gene="xthA"
                     /locus_tag="ACHKCL_001689"
     CDS             6510..7316
                     /gene="xthA"
                     /locus_tag="ACHKCL_001689"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5762678.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7483..8193
                     /gene="ydjX"
                     /locus_tag="ACHKCL_001690"
     CDS             7483..8193
                     /gene="ydjX"
                     /locus_tag="ACHKCL_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5762679.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            8198..8875
                     /gene="ydjY"
                     /locus_tag="ACHKCL_001691"
     CDS             8198..8875
                     /gene="ydjY"
                     /locus_tag="ACHKCL_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5762680.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            8890..9597
                     /gene="ydjZ"
                     /locus_tag="ACHKCL_001692"
     CDS             8890..9597
                     /gene="ydjZ"
                     /locus_tag="ACHKCL_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO5762681.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9597..10145
                     /gene="ynjA"
                     /locus_tag="ACHKCL_001693"
     CDS             9597..10145
                     /gene="ynjA"
                     /locus_tag="ACHKCL_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5762682.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10155..11321
                     /gene="ynjB"
                     /locus_tag="ACHKCL_001694"
     CDS             10155..11321
                     /gene="ynjB"
                     /locus_tag="ACHKCL_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5762683.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11294..12829
                     /gene="ynjC"
                     /locus_tag="ACHKCL_001695"
     CDS             11294..12829
                     /gene="ynjC"
                     /locus_tag="ACHKCL_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5762684.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12829..13482
                     /gene="ynjD"
                     /locus_tag="ACHKCL_001696"
     CDS             12829..13482
                     /gene="ynjD"
                     /locus_tag="ACHKCL_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5762685.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13549..14856
                     /gene="ynjE"
                     /locus_tag="ACHKCL_001697"
     CDS             13549..14856
                     /gene="ynjE"
                     /locus_tag="ACHKCL_001697"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5762686.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14865..15485)
                     /gene="ynjF"
                     /locus_tag="ACHKCL_001698"
     CDS             complement(14865..15485)
                     /gene="ynjF"
                     /locus_tag="ACHKCL_001698"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5762687.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15572..15979
                     /gene="nudG"
                     /locus_tag="ACHKCL_001699"
     CDS             15572..15979
                     /gene="nudG"
                     /locus_tag="ACHKCL_001699"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5762688.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(15945..16217)
                     /gene="ynjH"
                     /locus_tag="ACHKCL_001700"
     CDS             complement(15945..16217)
                     /gene="ynjH"
                     /locus_tag="ACHKCL_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5762689.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16453..17796
                     /gene="gdhA"
                     /locus_tag="ACHKCL_001701"
     CDS             16453..17796
                     /gene="gdhA"
                     /locus_tag="ACHKCL_001701"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5762690.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(17913..18953)
                     /gene="ynjI"
                     /locus_tag="ACHKCL_001702"
     CDS             complement(17913..18953)
                     /gene="ynjI"
                     /locus_tag="ACHKCL_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5762691.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(19081..21042)
                     /gene="topB"
                     /locus_tag="ACHKCL_001703"
     CDS             complement(19081..21042)
                     /gene="topB"
                     /locus_tag="ACHKCL_001703"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5762692.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(21047..22090)
                     /gene="selD"
                     /locus_tag="ACHKCL_001704"
     CDS             complement(21047..22090)
                     /gene="selD"
                     /locus_tag="ACHKCL_001704"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5762693.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(22207..22758)
                     /gene="ydjA"
                     /locus_tag="ACHKCL_001705"
     CDS             complement(22207..22758)
                     /gene="ydjA"
                     /locus_tag="ACHKCL_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5762694.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22919..24775
                     /gene="sppA"
                     /locus_tag="ACHKCL_001706"
     CDS             22919..24775
                     /gene="sppA"
                     /locus_tag="ACHKCL_001706"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5762695.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            24891..25601
                     /locus_tag="ACHKCL_001707"
     CDS             24891..25601
                     /locus_tag="ACHKCL_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO5762696.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            25733..26749
                     /gene="ansA"
                     /locus_tag="ACHKCL_001708"
     CDS             25733..26749
                     /gene="ansA"
                     /locus_tag="ACHKCL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5762697.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            26760..27401
                     /gene="pncA"
                     /locus_tag="ACHKCL_001709"
     CDS             26760..27401
                     /gene="pncA"
                     /locus_tag="ACHKCL_001709"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5762698.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(27494..28753)
                     /gene="ydjE"
                     /locus_tag="ACHKCL_001710"
                     /pseudo
     CDS             complement(27494..28753)
                     /gene="ydjE"
                     /locus_tag="ACHKCL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6949 a         7734 c         7331 g         6839 t
ORIGIN      
        1 acttaatacc cgcagaaaga tttctgcggg taagtattag cttatttttt cgagcattaa
       61 tcccgcgcgt aatcccagcg ctaccaacgg attagggaat aacacatact ctacatcatg
      121 ggttacggta aaacgttcct caccgtcctg cgccagcaac gttcctttct taaacggcat
      181 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
      241 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
      301 cagcgcagcg attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
      361 caacgctttg ccaagttcca gcgtacaggc cagcgcgcca aaatgtctgg cgctgaaatg
      421 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
      481 ccacgtcagg aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
      541 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc cagcgcacag attctttgcc
      601 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttccccgct
      661 ttcagcaaac agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
      721 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc accgtaacgg
      781 gatctcgccg tgagatatcg cgccaagcaa tgcatcaagc atctccacag gtgccgtctc
      841 attaccgtgg atccccgcag aaatcaccag agcgccttgc ggtggcgtta atggcgtcag
      901 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
      961 gacaggtttt ttactcgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     1021 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     1081 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     1141 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     1201 tcaccgcccg gcgttcttct tgtgtcaata ccacccgcaa ccgcaggcac gccgggccac
     1261 cgccattcgc catgctttcc cgtaaatcaa agacttttag ttcgctaatc gggttgtcag
     1321 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     1381 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agatatgtag
     1441 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     1501 cccgcgcacg caggtttgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa
     1561 acagcacctg gcggttactc acggcaatca cgtcattatg aaaaacgccc tggtcgataa
     1621 cgtccgggtt ttgctgggcg aaaatcacct gttggggatt cacctgattc agccttgcca
     1681 ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc
     1741 cctcttctcg cccgtagaca aaaagttgca tacccggttc accgtaatga ccgccgagac
     1801 gattgtggtt tgccgccccc tcatcaccga gcaaggctac ctgcggcaac gccgaatgga
     1861 cactaaattt ctcttcgtcg ttaaaaatcg cttttaacag cgattcagtg acgggcgctt
     1921 ccagcgaacg gtgaaattta ttgttcaggt tagcaacggt gagatgcact ttgccatcca
     1981 gcgtatcggc agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac
     2041 tgacgctgga aagccagtgc ggtgcctggc gtacaacctt ttccagtacc tgctcatcgc
     2101 taccactgaa tcccaactga cgcagcaccg gaataaacgg acgctcgtga ggcgggatca
     2161 cggcctgggg gaatcccgca tcggcaaggg ttttcatttt cagtaagccc tgttttgccg
     2221 ccagtcgcgg gttagagacc tgaaaacggt gacgggtaga ggctttatta ccaaacgaca
     2281 ggcccgcgta atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca
     2341 tcgcaccacc tcatcggaaa aatccagccc ggggttaagc gttgcgggca aagttaacga
     2401 gtccgactcc aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg
     2461 gcgatggtta ccggatgcac caatgccgcc gaatggcgcg gtactggcag caccagtaag
     2521 cggtttgttc cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc
     2581 ccgctcgggg gaaaccagac cgcaagagag tccgaagcga gtgttattcg ccattcgaat
     2641 cgcttcctcg aaagaatcat aacgccagac gcgcaataac ggtccgaaca cctcttcatc
     2701 tggtacgcca gcaacgcctg tcatttcaat gatccccggc gtcagcaacg atgtccctgc
     2761 ttgtaataag cgcggcgcaa gcagggttcg tccacccatc gcttccagtt gctgccaggc
     2821 agtaaacacc tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc
     2881 gtcatcccag ttgcccggcg ttaatcgctg gctgacggca accagacgag caagaaatgc
     2941 atcgccctgc gccccgcttt tcagcaatac acggcgggcg caggtgcagc gttgcccggc
     3001 tgtaacaaac gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc
     3061 gataatcagc gggttattgc cgcccatctc aagggcgaga attttctccg gctgaccgga
     3121 gagctggcga tgcagctggt agccggtatt ggcgctgccg gtaaacagca agccgtcgag
     3181 atcctccagc gcactcagcg cctgacccgt ttcacacccg ccctgcacca ggttcagcac
     3241 gcctggcggc aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt
     3301 cagttcgctg ggtttaaaga taatggtgtt acctgccagc aatgcgggaa cgatatgtcc
     3361 gttcggcaaa tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg
     3421 atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg
     3481 atacgcctta attgatatcg cgattttatt gatcatcgcc gtcaattcgg ttgccgcctc
     3541 ccagcgcggc ttgccggttt ctcgggcaat gattgcggtt aattcgcctt tattcctttc
     3601 cagcaatccg gcaaagcgtt cgacaacggc ctgacgttca gcaaatgaga gccgcgccca
     3661 gcgcggaaac gccgcacggg ctgcccgaca agcctgcccg acctgagcgg catcggcatc
     3721 attgccttgc cataacacct cgcccgatac cggattacgc ttcacacgcg atgcaccctg
     3781 gcccgttatc cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg
     3841 cgcaccagac gaacgcgatc ccctgcatgg catttgaggg catccagttg tgcggcggtt
     3901 aaaatcaaac gctcggttgc cggatcggta cgcaccagca ccacgcggaa atggtgataa
     3961 ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact
     4021 tccaccagcc gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgtcggc
     4081 ccaccgtcga agatgtcgat atagttgcgg tagcgaaaac cttctttctc cagcaccgcg
     4141 cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa
     4201 aagtgcgtat agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg
     4261 ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc
     4321 cagaacggtg aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca
     4381 ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg
     4441 ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg
     4501 tgatcgttac tgagaaacaa cgtcggcaat gcgttataga cattcagctc ttttgaggcg
     4561 tgaaccaacg tgccgacgcg atagttgtac cagggatcgt tcagcccaac tgccacctca
     4621 atggcgcaaa tccccgccac ggtgcctgtc tcgctatctt ccagtacaaa cacataaccc
     4681 tgctcacttt tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa
     4741 agcgtggctt cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc
     4801 atcagcgccg agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc
     4861 taacaaagtg ttcgcaagca actgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc
     4921 tgacattgag cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc
     4981 ctgctttcgc cgcttcctga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta
     5041 cacagccaat cagcaaacct aagccgcgaa cttcattgaa caaaccatag cggtgattaa
     5101 taatattaag acgctcaaca aaccagtcgt gacgttgctt aaccccgtta agcatctctg
     5161 gcgtattgat gagctccagc actttgcctg ccaccgccga ggccagcgga ttgccgccat
     5221 aggtggtgcc atgagtgcca acggtcatca cgctggcgca ctcttcggtt gctaacaacg
     5281 caccgaccgg gaaaccgccg cccagagctt tggcggtagt taacagatca ggcgtcacgc
     5341 cgtagtgcat ataggcatac agttccccgg tacgcccgac gccggtttgt acttcatcaa
     5401 aaatcaacag cgcattgtgg cgatcacaca attcacgcag accttgtaaa aatgcgttgc
     5461 tggctggcac cacaccgcct tcaccctgga tgggttcgac aatcaccgca caggtagagt
     5521 cgtcaatcag cgcgctggca gagttaatat cgttatatgc agcatgacgg atatctggtg
     5581 gcagtggcgc aaaatcctgt gaataggctg gctgcccacc cgcactaaca gtaaacagcg
     5641 tgcgaccatg aaacgcattt ttaaacgcca cgataccgct cttatggctg ccgtagcggt
     5701 cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac
     5761 aaaagaaaac gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg
     5821 actcgttggt gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgcct
     5881 cacgcagttc cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga
     5941 tatactcttt cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaacg
     6001 gtgccggagc gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag
     6061 acatagcgac ctctacagta aatagctcgt tatttatatg ttaataataa gtaatgtttt
     6121 cgctgtaaat gtagattgca gggttcgtgc cagccagtga tgaaagtgca taaacggcgg
     6181 aggctaactg gaaatcaagg agttataacc aaaccatatg catttaaagt gcatattaag
     6241 tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt
     6301 aaaatataat tctggaattg tgatcattga cgaaatttac tggaaattac tgcgccattc
     6361 tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc
     6421 atcgatcaaa tcacttaaca acaggcggta agcaaagcga aattctgcta ccatccacgc
     6481 actctttatc tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaatggcc
     6541 tgcgcgccag accccaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg
     6601 gcctgcagga gacaaaagtt catgacgata tgtttcccct cgaagaggtg gcgaagctcg
     6661 gctacaacgt gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag
     6721 agacgccgat tgccgtgcgt cgcggctttc ccggtgacga agaagaggcg cagcggcgga
     6781 ttattatggc ggaaatcccc tcgccgctgg gcaatgtcac cgtgatcaac ggttacttcc
     6841 cgcagggtga aagccgcgac catccgataa aattcccggc aaaggcgcag ttttatcaga
     6901 atctgcaaaa ctacctggaa accgaactca agcgtgaaaa tccggtgctg attatgggcg
     6961 atatgaatat cagtcctggc gacctggata ttggcattgg cgaagagaac cgtaagcgct
     7021 ggctgcgtac cggaaaatgt tctttccttc cggaggaacg cgaatggatg gagagactga
     7081 tgagctgggg attggttgat accttccgcc atgcgaatcc gcaaacagca gatcgtttct
     7141 catggtttga ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc
     7201 tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaatcc
     7261 gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta
     7321 gcagctctcc tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg
     7381 tcagtaatga tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct
     7441 gaccacttct ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaaa
     7501 tttctttttg cctgtcttat ttttgcgctg gccatttacg ctatccacgc tttcggttta
     7561 ttcgatctgc tcaccgattt accccactta cagacactca ttcgccagag cggattgttc
     7621 ggttatagcc tctatattct gttattcatc attgccaccc tctttctgtt accaggaagc
     7681 atattggtga tcgcgggagg aatagttttt ggcccgttct tagggacact actctcatta
     7741 attgccgcca cgctggcctc ctcgtgctca ttcctgctgg cgcgctggat gggacgtgat
     7801 ttactgctga aatacgttgg ccatagccat accttccagg ccattgaaaa aggcattgcg
     7861 cgtaacggta ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt
     7921 caaaattacg cttacggatt aaccacaatc gccttctggc cttataccct tatttcggca
     7981 ctcacgaccc tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa
     8041 ggcattacgc tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc
     8101 gtccagctcg caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc
     8161 aacccactta ctcacccaaa aaatgaagga tagaacgatg ttgcaacatt attcagtgtc
     8221 atggaaaaaa ggactgactg cactctgctt actggctgtt gctgggctta gcggctgcga
     8281 tcaaaaagag aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct
     8341 gcgcgtcgat gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct
     8401 caccgacgac actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct
     8461 gtttatgggt tatgcgaccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac
     8521 accgggcgaa aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact
     8581 ggatatttcg gtcaactggc aaggggcggc aaaaacgtat tccttcgatg aagtgattgt
     8641 tgacagtaat ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga
     8701 gaagaaaacg ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc
     8761 aacatacact tatggtgcgg ttgaaaaacg aggtgaagtt aaattcaaag gcaatgcctc
     8821 ggttctcccg gcgaataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca
     8881 ggataaagga tgatgaagat gcaatcgcgt aaaatctggt actaccgtat aaccctcatc
     8941 atcctgttgt tcgctatgct gctggcatgg gcgctgcttc ccggcgtcca tgagtttatc
     9001 aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag
     9061 tcttacggcg cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct
     9121 gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg
     9181 ggcggctcgc tgtcgtggac cagttcgatg gccggcgcgg cactgtgctt ttttatcgcc
     9241 agagtgatgg gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtact tgacagtatg
     9301 gacggctttt tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt
     9361 gtccctttcg atccaatcag ctatgctgcc ggtttgactt caatacgttt tcgctcgttt
     9421 tttatcgcca ccgggcttgg tcagttaccg gcgactattg tttattcctg ggcgggcagc
     9481 atgttaacag gcggtacctt ctggtttgtc accggactgt ttattctgtt tgccctgacc
     9541 gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgcctgatgg
     9601 gtttaccgcc gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc
     9661 gccattacgg cgaggtgcta agccccattc gctggtgggg gcggatcccg tttatctttt
     9721 atctggtgtc gatgtttgtt ggctggctgg agcgcaaacg ctcaccgctc gatccggtag
     9781 tacgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgtgtggata
     9841 tcaccagtat gaaagtcgcc gagcgcaccg gcagcagcga taaactgctg gcagtggctg
     9901 actggcggca aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag
     9961 ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg tacccgactg gctgcgcatt
    10021 ttgacgctca ggagctcacc gaactgacgg cattgatcgg cctgcaaaat ctgtcagccc
    10081 gttttaattc tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt
    10141 cttaaggaga gatgatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctc
    10201 tggcaacaca tgccagtgac tggcaagaaa ttaaaaatga ggccaaaggg caaactgtct
    10261 ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg
    10321 agatgaaaac ccattacgct ataaacctga agattgttcg tctggcggat gccgcagacg
    10381 cggtgaagcg cattcagacc gaagctgctg ccggacgtaa aacgggcggc tcggtggatc
    10441 tgctctgggt gaacggcgaa aacttccgca ccttaaaaga ggccaattta ctgcaaaccg
    10501 actgggcaga gactctgccc aactggcgct atgtcgacac acagctgccg gtgcgggaag
    10561 atttttcagt gcctacagaa ggggctgaat cgccctgggg gggcgcacaa ctgacattta
    10621 tcgcccgccg cgatgttacg ccacagccac cacaaacgcc gcaagcctta ctggagtttg
    10681 ctaaagccaa tcccggcacg gttacctatc cgcgcccacc ggactttacc ggcacggcgt
    10741 ttcttgaaca gttgctgatt atgctgacgc ccgatcccgc cgcattaaat gaagcgccga
    10801 acgatgcgac tttcgcccgt gtcactgctc ccttgtggca atatcttgat gcgctgcatc
    10861 cgtatttgtg gcgcgaagga aaggatttcc cgccttcgcc cgcgcggatg gatgctctgc
    10921 tgaaagccgg aacatttcgc ctgtcgctga cctttaaccc cgcgcatgcg cagcaaaaaa
    10981 tcgccagcgg cgatttgcct gcaagcagtt acagttttgg ctttcgcgag gggatgattg
    11041 gcaacgtgca tttcgtcacc attcctgcca acgcgaatgc cagtgctgcg gcgaaggtag
    11101 ttgccaattt cttgctctca cccgatgcgc aattgcgtaa agcagatccc gctgtctggg
    11161 gcgatccttc tgttctcgat ccgcaaaaac tgcctgatgg gcagcgcgaa acattgcaat
    11221 caagaatgcc gcaagatctg ccgccggtac tggctgaacc gcacgcaggt tgggtaaatg
    11281 cgctggaaca agaatggcta caccgttacg gtacgcatta atctttttgc tgtgggcgat
    11341 ggtggcggtg atttatgcac cgctgatccc ggcagctctc acgctgatat cgcctgcttt
    11401 gtcgttgaca cactggcagg cgttatttgc cgatccacag ttaccgcagg cattactggc
    11461 aacgctggtg tcgacaacca tcgcggcggt cggggcgttg ttgattgccc tactggtgat
    11521 tgtggcgctg tggcctgggc cgaaatggca gcgtatgtgc acccgtctgc cgtggctgct
    11581 cgccattccc catgtggctt ttgccaccag cgcccttctg ctctttgctg acggagggct
    11641 gctttatgac tatttcccgt atttcactcc gccaatggac aaattgggta ttgggctggg
    11701 cttcacgctg gcggtgaaag aaagcgcatt tctgctgtgg atcttagcag cggtattgag
    11761 tgaaaaacgg cttttacagc aagtcatcgt gctggattcg ctgggctaca gccgctggca
    11821 atgcctgaac tggctgctgt tgccctccgt cgcgcctgcg ctggcaatgg cgatgctggc
    11881 gattgttgcc tggtcgctgt cggtcgtaga tgtggcgatt attcttgggc caggtaatcc
    11941 accgacgctg gcagtaatta gctggcagtg gttaactcag ggcgatgccg atcaacaaac
    12001 gaaaggcgca ctcgccagcc tgctgctgat gttgttactc gccgcctacg ttttgctggg
    12061 ctatctgcta tggtgtacct ggcggcgtac tattccccgc gtagatggcg ttcgtaagcc
    12121 tgccacgcct ttattgccag gcattacgct ggcgagtttt ttacccttga ccagtgtgct
    12181 gtgtgtggtt ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg aagcgctcat
    12241 caacagcctg acaatggggc tggtggcgac attcatcgct ttgctcctgt tactgctgtg
    12301 gctggaatgg gggccacagc gtcgccagtt gtggctatgg ttgccgattt tattaccggc
    12361 actgccgctg gtggcgggcc agtacacgct ggcgctatgg ctgaacatgg atggaagctg
    12421 gacggcggtg gtctgggggc atctgctgtg ggtaatgccg tggatgctgt ttatcctgca
    12481 acccgcctgg cagcgcattg attcacggtt aattttgatt gcacaaacac tgggctggtc
    12541 gcgggccaaa atcttctttt acgtgaaatg tccgcttatg ctacgcccta cgctgattgc
    12601 cttcgcggtg ggattttcag tcagtattgc gcagtatatg ccgacgctgt ggctgggcgc
    12661 ggggcgtttt ccgacgctca ccactgaggc ggtggcatta agcagcggcg gcagcaacgg
    12721 tattctcgcc gccctggctt tatggcaact gctattaccg cttattattt ttgccctgac
    12781 cgcgttagtc gcaaaatggg taggttatgt cagacaagga ctccgctaat gctctgcgtg
    12841 aaaaatgttt cgctacgttt accagaaagt tgcttgctga caaacgttaa ctttacggtg
    12901 gataaaggtg acattgtcac gttaatgggg ccgtctggct gtggaaaatc cactctgttt
    12961 tcatggatga ttggtgcact ggccggacag ttttcttgta caggtgagct atggctcaat
    13021 gagcaacgga ttgacatgct acccaccgca cagcgtcaga ttggcattct ttttcaggat
    13081 gcactgttat ttgaccagtt cagtgtcggg caaaatttac tgctggcgct accggcgaca
    13141 cttaagggga atgcccgacg taatgccgtg aatgatgcac ttgagcgttc aggccttgac
    13201 ggagctttcc atcaggatcc tgctactttg tctggcggtc agcgagcgcg cgttgctctg
    13261 cttcgcgccc ttctcgctca gccaaaagcg ttgctactgg atgagccatt cagccgtctt
    13321 gatgtggctc tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg cgaactggcg
    13381 atccccgtcg ttcaggtaac gcacgatctc caggatgttc ctgctgatag ttctgttctg
    13441 gatatggcgc agtggtcaga aaattacaac aaactgcgat aacgcaaagt ttttctcaat
    13501 gcgtcagttc agaatggcgc actcaaaact acaatgtcgg gattttcgat gaaacgtgtt
    13561 tctcaaatga ccgcgctggc aatggcttta gggctggctt gcgcttcttc gtgggccgct
    13621 gaactggcga agcctcttac acttgaccag cttcaacaac aaaatggcaa agcgatagat
    13681 actcgctcca gcgcgtttta taacggctgg ccacaaacct taaatggccc ttctggtcat
    13741 gaacctgccg ccttaaacct ctctgctagc tggcttgaca aaatgagcac cgaacagctc
    13801 aacgcgtgga tcaagcaaca taacctgaaa gccgatgccc cggtggcgct gtacggtaat
    13861 ggcaaagatg tcgacgccgt caaaacgcga ctgcaaaaag caggttttac gcatatctcc
    13921 attctgagtg acgcgctaag cgaaccttcc cgtctacaaa aactgccgca ctttgagcag
    13981 ctggtttatc cgcaatggct gcacgacctg caacaaggta aagaggttac ggcgaaacct
    14041 gccggtgact ggaaagtcat tgaagcggcc aggggcgctc ctaagcttta ccttatcagc
    14101 catattcccg gtgctgacta catcgatacc aacgaagtgg aaagcgaacc gctgtggaac
    14161 aaagtttctg atgaacaact aaaagcgatg ctggcaaaac acggcattcg ccatgacacc
    14221 acggtcattc tgtacgggcg tgacgtatac gctgcagcgc gtgtggcgca gattatgctt
    14281 tatgccggcg tgaaagatgt gcgcctgctg gatggcggct ggcaaacctg gtccgacgcg
    14341 ggtctgcccg ttgagcgcgg tacgccaccg aaagtgaaag cggaaccgga tttcggcgtg
    14401 aagatcccgg cacaaccgca gttgatgctc gatatggaac aggcccgtgg actgctgcat
    14461 cgccaggatg catcgctggt gagcattcgt tcgtggccag aatttatcgg tacgaccagc
    14521 ggttacagct atattaaacc aaaaggtgaa atagccggag cacgttgggg acacgctggt
    14581 agcgactcga cgcatatgga agatttccat aatccggatg gcaccatgcg tagcgccgat
    14641 gatattaccg ctatgtggaa agcatggaat atcaaaccag atcagcaagt ttcattctac
    14701 tgcggcaccg gctggcgcgc gtccgaaacc tttatgtacg cacgagcaat gggctggaat
    14761 aacgtctccg tttacgacgg cggctggtac gaatggagca gcgatccaaa aaatccggta
    14821 gcaaccggtg aacgcggccc ggacagcagc aaataacatt gcaattactg acgctggagc
    14881 gacttcagcg tcagataccc actccacacc cgcgtaaagg tcgtcatcca gcacaacgca
    14941 ccgaagatcc acgcaaacca cgcaaaccat gcagggaata aacatcccag cacaaacagt
    15001 aagatcgttt ctgtgccttc ggttaatccc cccagataat aaaacgattt atgcgcataa
    15061 ccgggattat caatctgata tttcgccgcc agcgcagcaa aagcgagaaa actgctaccc
    15121 gtgccgataa acgcaaacag cagccagccg cctgccaggg cgttttgctg cggtgcggcg
    15181 aggataaacc caaaaggtac cagcgcgtaa aaaagaaaat cgagagaaat atcaagaaag
    15241 ccgcccgcgt cggtaagccc tctgcgccgc gccagcgcac cgtccagacc atcaagcagc
    15301 ctgttcaaca aaatgacgac aagcgccgcc agataccagc ccagcgccag aaaaggcagt
    15361 gccagcacac cgatggcaaa tcccactaat gttaaaccat ctggcgtaat gcccggttta
    15421 tcaagaaccc gcacacactg atgcaacaac ggtttaatcc ggggatgaag atggcggtct
    15481 agcacaggca ctccttaaat ataaagcctt tctgattgag caacagtgcg gatattatgg
    15541 catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg ttgccgccat
    15601 cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg atcaggcggg
    15661 attatgggag tttgccggtg gtaaagtcga gccggatgaa agccagcagc aggcgctggt
    15721 gcgtgagtta aacgaagaat tgggcatcga agcaactgtg ggtgagtatg ttgccagcca
    15781 tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc tggcacgtac ccgacttcca
    15841 cgggacgtta caggcacatg aacatcaggc gctggtctgg tgctcacctg aagaggcgct
    15901 gcaatatccg ctggctcctg ctgacattcc attattagag gcgtttatgg ctttacgcgc
    15961 cgccagacca gcggattagt gctaagggtt ttgtcatccc gctggcattg cagcagtatt
    16021 ccttcggctt taattaccgc cccttcagaa taatttttat cctgataaac gcagcactga
    16081 gtacagggct gcgctgactg cccgcctgaa ctgaccactt ctggcggtac gtttacctcc
    16141 acgtccggac cataatgcgg gttagccagt gcaattaatg gaaatgctaa tactacggcg
    16201 aacaatgctc gactcacagg gaactcctta acgttattgt ctctgctact gataacggta
    16261 gccgggtggc aaaactttag cgtctgagtt atcgcatttg gttatgagat tactctcgtt
    16321 attaatttgc tttcctgggt catttttttc ttgcttaccg tcacattctt gatggtatag
    16381 tcgaaaactg caaaagcaca tgacataaac aacataagca caatcgtatt aatatataag
    16441 ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca tgtccaaaag
    16501 cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac cacactctgg
    16561 ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg tctggttgaa
    16621 ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaatca ggtacaggtc
    16681 aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc cgtacaaagg cggtatgcgc
    16741 ttccatccgt cagttaacct ttccattctt aaattcctcg gcttcgaaca aaccttcaaa
    16801 aatgccctga ctactctgcc gatgggcggt ggtaaaggcg gcagcgattt cgacccgaaa
    16861 gggaaaagcg aaggcgaagt gatgcgtttc tgccaggcgc tgatgactga actgtatcgc
    16921 cacctgggcg cggataccga cgttccggca ggtgatatcg gggttggtgg tcgtgaagtc
    16981 ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt cttcaccggt
    17041 aagggccttt catttggcgg cagtcttatt cgcccggaag ctaccggcta cggtctggtt
    17101 tatttcacag aagcaatgct aaaacgccac ggtatgggtt ttgaaggcat gcgcgtttcc
    17161 gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga atttggtgct
    17221 cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg attcacgaaa
    17281 gagaaactgg cacgtcttat cgaaatcaaa tccagccgcg atggtcgagt ggcagattac
    17341 gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt accggttgat
    17401 atcgccctgc cttgcgctac ccagaacgaa ctggatgttg acgccgcgca tcagcttatc
    17461 gctaatggcg ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat cgaagcgact
    17521 gaactgttcc agcaggcagg cgtactattt gcaccgggta aagcggctaa tgctggtggc
    17581 gtcgctacat cgggcctgga aatggcacaa aacgctgcgc gcctgggctg gaaagccgag
    17641 aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg tgttgagcat
    17701 ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg ttttgtgaag
    17761 gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg atggcgctac
    17821 gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg taggccgggc
    17881 aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga tgatgctccc
    17941 cagggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca aaactgcttg
    18001 tcgtaacgat aacaagcttc aagttcagca tccgttaact ttctgcgata gcagcagata
    18061 tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac
    18121 tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc ttcaattgtc
    18181 ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt
    18241 agcttcgccg aggatttacc ggagctatga ttagcgcaat cagagatata gtctgaggga
    18301 aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct
    18361 ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataactt
    18421 tcgctatctt cgatacccca ttgttcctct aaagactcgc gtcttttact tatgatatcg
    18481 atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc aggctttcgc
    18541 ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg tgcgatcaat
    18601 tgtagcgcct gtaatttctc gatactgagc ggctcaatac ctttagcctg ataataatgc
    18661 agctgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat aattaataga
    18721 taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt ggatgttatc
    18781 gctgcgaata caaacattac agcgacaaca catgttaaat aaaaccccca cttacaaagc
    18841 agcatggtct tattttcttt aatcatccgt tcaaaattac tattaaatat ttcccagcca
    18901 ttaaaagaat acttctcgct cccaggatgg ttttgtaata aaactttttt catcacattc
    18961 cctgttatta catactttag aggcgataat tttatccatg caaaaaaaat atccaacgaa
    19021 ttttcttgat ccgctggata tttcagaata tgactcgaat agcacgaaag attcgctcgc
    19081 ttacgctatc gccccgcttc cggcttcatc tgctggcgga ctttttttcg cactacgttt
    19141 acgcggtgca gcctttttct tatcagcact gccaccactg cccggagcca caatgccgcg
    19201 aaactgccgc accggagtac gtttggcttg atcaataagc tgatatagcg tccccaccag
    19261 cggctgcata aagtcctgat agcgacactg cttttcgctg atttgcgtca gcaccgattc
    19321 ccagtgcgcg gtcatgtccg gtcgcgtagc catttctggc agcgaatgga ataacgcttt
    19381 tccggcgtcg gtggagtgga tatagcgccc ttttttgatg aggaaaccac gcttgaacaa
    19441 cagttcaata atcccggcac gcgttgcttc tgtccccaga ccatcggtcg cacgaaggat
    19501 ctttttcaga tctttgtcct gcacaaagcg cgcgatcccg gtcatcgccg aaagcagtgt
    19561 tgcatcggta aaatggcgcg gcggctgggt ttgccgctct accacttcac ctttttcaca
    19621 cagcaactca tcgcctttcg ccaccacagg caatggcgta ccgtcgtttt cttcatcgcg
    19681 ctctttgctg cctaacagcg tgcgccagcc tgcttcagca agaaaacgcg ctttagcgac
    19741 aaatttgcct ttggcaatgt ccagttcgat aacacacttg cggaacaccg catccgggca
    19801 gaactgcatc agatactgac gggaaatcag gttatagacc ttcgcttcgt tctccgtcag
    19861 gttgatcgca gaactccgtg cggtcggaat gatggcgtgg tgcgcatcga cctttttgtc
    19921 atcccaacag cggttgcgta tatctggatc taccactggc tgcggcaaca gatctggtgc
    19981 atgaacgctg atggcattca tcaccgcgtg gcgtccggca aaatgttctt ctggcaaata
    20041 gcgacaatcg gaacgcggat aagtgattag cttgtgcgtt tcgtacagct tctggcagat
    20101 atcaagcacg ttctgcgcgc tcagaccaaa gcgttttgcc gcttcaatct gcaacgccga
    20161 aagcgaaaac ggcagcggcg cggattctga ttcccgttta tcgttatagc tggtgacaat
    20221 agccggttga ccactaatgc ggttaaccac atgctccgcc agtggacgat gtaacaagcg
    20281 cccttcttca tcctggtacg gttcacacgc ttcgctcggt tgccagatag cggtaaaccg
    20341 ctcatcggca ggtgtcacga tatgtgcttt gacttcaaag aagtctttcg ccacgaagtt
    20401 ttcaatctct tcatcgcggc gcaccaccag cccaagcacg ggcgtctgca cgcgtcccac
    20461 ggaaagcacg ccctgataac cggcattgcg accgagaatg gtatacgcac gggtcatatt
    20521 gatgccgtac agccagtcgg cacgcgctcg cgccagcgca gaaacgcaca gcggtacaaa
    20581 ctcactgttg gaacgaagac ggtcgatcgc ccgctcaacc gcctgcgggt tcaggtcgtt
    20641 tatcaagcaa cgctgcacct gctggcgctt ttccggtgcc agttgtagat agtccagcac
    20701 ttcatccacc agcagttgcc cttcgcgatc cgggtccccg gcgtgaacga tttcgctggc
    20761 ttcatgcagg taccgtttga tgacgttaag ttgtttggtc acggagggtc ggggctgtaa
    20821 ttgccacttt tccgggacaa tcggcaaatc cgcaagattc cagcgcgcat agcggctgtc
    20881 gtaggcgtct ggctgcgcct gctcaagcag gtgaccgata caccaggtca ccacctgacc
    20941 attaccgcac tcgataaagc catcgccttt ccggtgcggt ttgggcagga catcagcaat
    21001 ggcgcgcgcc agactcggtt tttcggcaat aaacaaccgc attgaattaa cggatctcaa
    21061 ccatggcacg accgccgcgt gctggcacca gttcgccaat cgccgtcagt tcaatgccaa
    21121 actcggcggc tgtagctttg acctcatttt cggcttccgg catgaccgcc agcagcaaac
    21181 cgccagaagt ttgcggatcg cacagcaaat cgcgcacttc acgcggcatt tcacccatca
    21241 gatgaccgta gctggcaaag ttacgttcgg tgccgccagg cactgcaccc aacttaatgt
    21301 actcttcaac ccccggcaat ttcgggatcg cgtcatagtc aacgcgcgcc tgcacacctg
    21361 cgccctgaca catttcgctc aaatggccca gcaggccaaa gcccgttacg tcggtcatcg
    21421 cttttacgcc ttcaatgttg gcaaaggacg cgcctgcgat gttcatccgg cacatcactt
    21481 ccgtcgccag tccctgatgt tctggtttca gcagtgattt tttctcagcc gtggtaagaa
    21541 cgccgatccc cagcggtttc gtcaggaaca gtttacatcc ggcttgtgcg gtactgtttt
    21601 tcttcacccg ctcggtcggt acgatccccg ttaccgccag accaaaaatc ggctccggcg
    21661 catcgatgga gtgaccgcca gccagcgcaa tacccgcctg acggcatgca tagcgtccac
    21721 cttcggttac ttcgcgggca atttccgggg aaagtttgtt aatcggccag ccgaggatcg
    21781 caattgccat aatcggtttg ccccccatcg cgaagatatc gctgatggcg ttagtcgccg
    21841 caatgcggcc aaaatcgaaa ggattatcaa cgatcggcat aaagaagtcg gtggtactga
    21901 taacgctggt gccattgccc agatcgtaca ccgccgcatc gtcgcgggtt tcattaccca
    21961 caagcaaatt cggatcaaca aacttcgcct gctcactgtg caggatggtt tccaacactt
    22021 ttggggaaat tttacagccg caaccagctc cgtggctgta ttgggtcaaa cgaatcgagt
    22081 tctcgctcat ggacatctcc tgtcaatgca atccgggtat ggtaaccctc attccgtgaa
    22141 gtgataagtg agagtgtctg aattcctgcg cctttgctca caatccagac agtttcgcga
    22201 caattatcag aaataagtga caaacggcgt cgggtccggg acgtttatcg acgtagatgc
    22261 tttcagctgc ggcgtgccga ggtagagaaa accgacaatt ttatcctgct cacggcaacc
    22321 gaatgcttca cgcactaccg gactttcagt taatgcgcca ctgcgccaga tgccgccaaa
    22381 cccctgggca acggctgcca tttgcatcgc catgaccgcg catccggcag acatttcctg
    22441 ttcccatcgc gggactttat gattttcttc gcatttcgcc accaccgtga tgatgagcgg
    22501 tgcgcggaac ggcgcattac gggctttgtc gatagctttg tcatcactac cggcagcaat
    22561 cgccccctgt tccagtacgg cgctgaaacg ctcgcgccct tccccttcaa tcacaaaaaa
    22621 atgccacggt tgcatggact tatggtccgg cgcgcgcata cccgcacgca ggatgttttg
    22681 cagttgttca cccgttggtg cgggttcagc caggcgggag gcgctacggc gattgatcaa
    22741 tagttcgagt gcatccattt gattaactcc tgtcgtgata tttattcaca aaattaacac
    22801 gagagtggat tttgttacag cacagtccgc aattcctgct gacaagtgcc ggttgggtca
    22861 ttacgataac cacatctatt gcgcctgtga caggtgtgac cttaagttgg gagaatacat
    22921 gcgaaccctt tggcgattta ttgccggatt ttttaaatgg acgtggcgtc tgctgaattt
    22981 cgtccgtgaa atggtactta acctgttctt tatttttctc gtgctggttg gtgtggggat
    23041 ctggatgcag gtcagtggtg gtgattcgaa agaaacggcc agtcgtggtg cactgctgct
    23101 ggacatttct ggtgtgatcg tcgataaacc cgacagttct cagcggttta gtaaattaag
    23161 ccgccaacta cttggtgcca gttccgatcg tctgcaggaa aactcactgt ttgatatcgt
    23221 caataccatt cgccaggcga aggatgaccg caatatcacc ggtatcgtga tggatctgaa
    23281 aaacttcgca ggcggcgacc aaccgtctat gcagtacatc ggcaaagccc tgaaagagtt
    23341 tcgtgacagc gggaaaccgg tttatgccgt tggcgagaac tacagccagg ggcaatatta
    23401 tctcgccagt ttcgccaata aaatttggct gtcaccgcaa ggcgtggtgg atttgcacgg
    23461 ttttgctacc aacggtctgt actacaaatc gttgctggat aagctgaaag tttccaccca
    23521 tgtgttccgc gtgggtacgt ataaatctgc cgttgaaccg tttattcgtg atgacatgtc
    23581 accggcagcc cgcgaagctg acagccgctg gattggtgag ctgtggcaaa actatctgaa
    23641 tactgttgcc gctaaccggc agatccctgc tcagcaggta ttccctggcg cgcaagggtt
    23701 gcttgagggt ttaaccaaaa ccggtggcga taccgcgaaa tatgcactgg aaaacaagct
    23761 ggtcgatgca ctggcatcga gtgcggaaat cgaaaaagca ctgaccaaag agttcggctg
    23821 gagtaaggct gataaaaatt atcgcgccat cagttattac gattacgcat tgaaaacgcc
    23881 ggcagatacc ggtgacagca tcggtgtcgt cttcgctaat ggcgcaatta tggatggcga
    23941 ggaaactcag gggaatgttg gcggtgatac cactgcggca caaatccgcg acgctcgcct
    24001 tgacccgaaa gtgaaagcga ttgtcctgcg tgttaatagc ccaggcggca gcgttaccgc
    24061 gtctgaagtg attcgcgctg aactggcagc agcccgggca gcgggtaagc ctgtggttgt
    24121 atcgatgggc ggcatggcgg catctggtgg ttactggatt tccacgccag ctaattacat
    24181 tgtggctaac cccagcaccc tgaccggttc tattggtatc ttcggcgtga tcaccaccgt
    24241 agaaaatagt ctggattcga ttggtgttca tactgatggt gtctcaactt caccgctggc
    24301 ggatgtttct atcaccaggg cactgccgcc ggaagcgcag cagatgatgc agttaagcat
    24361 tgagaatggc tataaacgct ttatcacgct ggttgctgat gcgcgtcatt cgacgccgga
    24421 gcagattgat aaaatcgccc agggccacgt ctggaccggt caggacgcaa aagctaacgg
    24481 gctggtcgat agtctcgggg atttcgatga tgcggtcgcc aaagcagcag agctggcaaa
    24541 agcgaaacag tggcatctgg aatactacgt tgatgaaccg accttcttcg acaaagtgat
    24601 ggacaacatg tctggttctg tccgggcaat gttgccagat gcgttccagg ccatgttacc
    24661 tgcaccgctg gcctcggtag cctctaccgt taaaagtgaa agcgacaagc tggcggcgtt
    24721 taacgaccca caaaaccgtt atgcgttttg cctgacctgc gccaacgtgc gttaaatcct
    24781 gcaaaaatgt ggccgatatg ctgtcggcca cactcctgaa tcggactgtc tttcactccc
    24841 tctctccact acattccctt gctcatttag catctgatat ggagtcatca gtgaacaagg
    24901 ccccctcttt aattgccgct atcgtcctcg gactggggat tagcgcctgt ggttattttg
    24961 ttggcgatgg tgttaaacat ttaaaaacca acaaccgtta tgtcaatgtg cgcgggcttt
    25021 ctgaaaaaga agttcgcgca gatacggcgg aattaacaat tgcgattaac tttaagggta
    25081 atgttcccgg cgaactgttc ccgaaactgg aagaagcgca gaaaaaaatt gttgccgaac
    25141 tcaatgctca ggggattaac gagaaagaga taattctggg ccaatggaca agcaagcgaa
    25201 cagattcctt ttatctgaaa gatgatccca caatgccacg ctataacgca gacgggtcag
    25261 tgacgataaa aacccataat gttgctgctg tcgaaaaagt cgtcgcgaaa ttaaacgaac
    25321 tgcaagtcgc tacggatggc gctatcgctg agagcaaagt cgcctatcgc tttaatggta
    25381 ttggcgcact gcgcgctgag atgattgctg ccgccaccaa agatgcacgc aacgccgcac
    25441 tacaatttgc aacggactca ggtagccagg tggggtcaat tagcgatgcc tcacaaggcg
    25501 tattccagat ctttgccagt ggcagtgatg aagatgatcc tacggcgatt aataaaacgg
    25561 tacgcgtagt cacgaccgta acctatgcgt tgcaggatta accacttttc tccggcggga
    25621 cactaatccc tccgtcctgc cggacttttc tcatatccac agagtatcca ggtgcgctcg
    25681 ctgcccctca cgtatatact tttgctcttt cgatatcatt catatcaata tcatgcaaaa
    25741 gaaatcaatt tacgttgcct acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg
    25801 ttatataccg gtgtcaggtc atctacaacg ccaactggcg ctgatgccgg aattccatcg
    25861 cccggagatg ccagatttca ccattcatga atatacgccg ctgatggatt cttcagatat
    25921 gacgccggaa gactggcagc atattgctga agatattaaa gcgcactatg acgactatga
    25981 tggttttgtc attctgcacg gcaccgacac gatggcgtat accgcctctg cgctgtcgtt
    26041 catgctcgaa aatctcggta aaccggtcat tgtgacaggg tcacaaatcc cgctggctga
    26101 gttacgctct gacggacaaa ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc
    26161 gatcaacgaa gtaacactct tttttaataa ccgactgtat cgcggcaacc gcactaccaa
    26221 agcccatgcc gatggttttg atgcgtttgc ctctccaaac cttcctccgt tactggaagc
    26281 aggtatccat attcgtcgtt tgaatacgcc acccgccccg cacggtgaag gggaattgat
    26341 cgtacatcca atcaccccac aaccaattgg cgtagtgacg atttatccag ggatttctgc
    26401 tgacgtcgtg cgcaattttc tgcgccaacc ggtgaaagca ttgattctgc gctcctatgg
    26461 cgtgggtaat gcgccacaaa acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg
    26521 cggtattgtg gtggtcaacc tgacacaatg tatgtccggt aaagtgaata tgggtggtta
    26581 tgccaccggt aacgccctcg cccatgctgg cgttattggc ggtgcagata tgactgtaga
    26641 agccacgcta accaaactgc attacctgct tagccaggaa ctggatactg aaaccattcg
    26701 caaggccatg agccaaaacc tgcgcggcga actgacgccg gatgattaag gagactgcaa
    26761 tgccccctcg cgccctgtta ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc
    26821 tcgccgtgcc ggaaggtgac agtacggtgg atgtcgctaa ccgcctgatt gactggtgcc
    26881 agtcgcgcgg tgaagcggtt atcgccagtc aggactggca cccggcgaat cacggcagtt
    26941 ttgccagcca gcacggtgta gagccttata cgccaggcca actcgacggt ttgccacaaa
    27001 ccttctggcc agatcactgt gtgcagaaca gtgaaggcgc acaattacat ccgttactga
    27061 accaaaaagc gatcgcagcg gtgttccata aaggcgaaaa tcctttagtt gacagttaca
    27121 gtgccttttt tgataacggc cgtcggcaga aaacctctct cgatgactgg ttacgcgatc
    27181 atgaaatcga tgaattgatc gttatgggcc tggctactga ctattgcgtg aagtttaccg
    27241 tgctggacgc gttacagtta ggttataagg taaacgtgat taccgatggt tgtcgtggcg
    27301 tgaatatcca gccccaggac agtgcgcacg cgtttatgga gatgtcagca gctggggcaa
    27361 cgctatatac gctggcagac tgggaagaga cgcaggggta attttacgct ggcctacaat
    27421 tctgtactgg cattgtaggc caaataaaac acgtcagtgg cacatctggc aattgatgcc
    27481 atcaacgaaa gatttaattt acctcagaaa tctcttccaa cgacaccttc cgcgtttcga
    27541 tgccaaaaat ggagagaacc agcgcacaga gcaataacat gacaccaagt accataaaca
    27601 ccgtgatcga cccataatgt gttaataatg ccgcaacgcc atagggcgtg aagactgcga
    27661 cgatccgtcc gacggcatta acgaaacccg aaccgcgcag gcgtaaatgc gttggccaaa
    27721 gctccgggat ataaaccgcc gacgcgaagc aaacatacat gtataaaaag aagatcatca
    27781 ccaggccata gattaaaatc gcccactctg tagtctgaat tgaatagata tagcctaaca
    27841 ctgcaataat aataagtaag gtggagccaa ataaccgacg aggaaaatga tcaataatta
    27901 atgccgcaat aaatattcct accggagcgc caatcataat aacagcggtc attaatattg
    27961 atttatcgac atcaatgccg gagttaacaa atatggtcgg tatccatacg gtgatggtat
    28021 aaagcgaaat gttcattgca attaaaacag taatcgcgac taatgtacgt cgtaacattt
    28081 cacctttaaa caggagccag aaagtaccct tgattacttt aaccttgctg ttgctctgat
    28141 acgaagtaag cgggggtaaa cgaatactct tctctctttc aatttgctgc tctacttcac
    28201 gaagttggca ttctgcacct gcgatttgcc ctttccctgc cagccatcgt ggcgactcaa
    28261 taaagtattt acctgagaga aaccaggcta acagtatgcc aataccaccc aacagaaaca
    28321 ttatccgcca actaaaaaaa gcgataacca ccacacctat cgccgcagac agcatgggcg
    28381 accagttacc aacaaatgag agccgcgcgg accattttcc acgcaccgtc gcggggataa
    28441 actccgtaaa tgaggcatag ccaaccataa tcagcgcccc cattcctgtt cccatcagga
    28501 agcgaaagaa gataagccag tacatatcag ggacaaaagc ggcccctgtt gcagcaatac
    28561 cgacgatgag aagatttatg cgaaacgccc tgcgccgccc aaagtaatca ccgatgaacc
    28621 caccggtaag agagccgatg aaataaccaa acattaatgc cgaggtaaag gcggcattga
    28681 ggaaattatt tgaccatcca ttgcttacca gctttgctaa gacgacatta ccggagtaac
    28741 tcaaaaaccc tgtgaaccgc cccgggaatc ctggagacta aactccctga gaaagaggta
    28801 aacaggatga ctaaaaatac acgtttttcc cccgaggtcc gtcaacgggc agt
//
LOCUS       DAZFKY010000044        28536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFKY010000044 DAZFKY010000000
VERSION     DAZFKY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(92..1459)
                     /gene="rhlE"
                     /locus_tag="ACHKCL_001711"
     CDS             complement(92..1459)
                     /gene="rhlE"
                     /locus_tag="ACHKCL_001711"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5762699.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1688..2359
                     /gene="cecR"
                     /locus_tag="ACHKCL_001712"
     CDS             1688..2359
                     /gene="cecR"
                     /locus_tag="ACHKCL_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5762700.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2362..3357
                     /gene="hlyD"
                     /locus_tag="ACHKCL_001713"
     CDS             2362..3357
                     /gene="hlyD"
                     /locus_tag="ACHKCL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5762701.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3350..5086
                     /gene="ybhF"
                     /locus_tag="ACHKCL_001714"
     CDS             3350..5086
                     /gene="ybhF"
                     /locus_tag="ACHKCL_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5762702.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5079..6212
                     /gene="ybhS"
                     /locus_tag="ACHKCL_001715"
     CDS             5079..6212
                     /gene="ybhS"
                     /locus_tag="ACHKCL_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5762703.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6223..7329
                     /gene="ybhR"
                     /locus_tag="ACHKCL_001716"
     CDS             6223..7329
                     /gene="ybhR"
                     /locus_tag="ACHKCL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5762704.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7291..7701)
                     /gene="ybhQ"
                     /locus_tag="ACHKCL_001717"
     CDS             complement(7291..7701)
                     /gene="ybhQ"
                     /locus_tag="ACHKCL_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5762705.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7834..8595
                     /gene="ybhP"
                     /locus_tag="ACHKCL_001718"
     CDS             7834..8595
                     /gene="ybhP"
                     /locus_tag="ACHKCL_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5762706.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8592..9833
                     /gene="clsB"
                     /locus_tag="ACHKCL_001719"
     CDS             8592..9833
                     /gene="clsB"
                     /locus_tag="ACHKCL_001719"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5762707.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9833..10789
                     /gene="ybhN"
                     /locus_tag="ACHKCL_001720"
     CDS             9833..10789
                     /gene="ybhN"
                     /locus_tag="ACHKCL_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5762708.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLAHFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10825..11538)
                     /gene="ybhM"
                     /locus_tag="ACHKCL_001721"
     CDS             complement(10825..11538)
                     /gene="ybhM"
                     /locus_tag="ACHKCL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5762709.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11746..12450)
                     /gene="ybhL"
                     /locus_tag="ACHKCL_001722"
     CDS             complement(11746..12450)
                     /gene="ybhL"
                     /locus_tag="ACHKCL_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5762710.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12586..13038)
                     /gene="moaE"
                     /locus_tag="ACHKCL_001723"
     CDS             complement(12586..13038)
                     /gene="moaE"
                     /locus_tag="ACHKCL_001723"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5762711.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGLVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13040..13285)
                     /gene="moaD"
                     /locus_tag="ACHKCL_001724"
     CDS             complement(13040..13285)
                     /gene="moaD"
                     /locus_tag="ACHKCL_001724"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5762712.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13278..13763)
                     /gene="moaC"
                     /locus_tag="ACHKCL_001725"
     CDS             complement(13278..13763)
                     /gene="moaC"
                     /locus_tag="ACHKCL_001725"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5762713.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13766..14278)
                     /gene="moaB"
                     /locus_tag="ACHKCL_001726"
     CDS             complement(13766..14278)
                     /gene="moaB"
                     /locus_tag="ACHKCL_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5762714.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14300..15289)
                     /gene="moaA"
                     /locus_tag="ACHKCL_001727"
     CDS             complement(14300..15289)
                     /gene="moaA"
                     /locus_tag="ACHKCL_001727"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5762715.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15277..15421)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15686..16594
                     /gene="yvcK"
                     /locus_tag="ACHKCL_001728"
     CDS             15686..16594
                     /gene="yvcK"
                     /locus_tag="ACHKCL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5762716.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16785..18806)
                     /gene="uvrB"
                     /locus_tag="ACHKCL_001729"
     CDS             complement(16785..18806)
                     /gene="uvrB"
                     /locus_tag="ACHKCL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5762717.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19385..20062)
                     /gene="bioD"
                     /locus_tag="ACHKCL_001730"
     CDS             complement(19385..20062)
                     /gene="bioD"
                     /locus_tag="ACHKCL_001730"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5762718.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGESPWLAENPENAATGK
                     YINLALL"
     gene            complement(20055..20810)
                     /gene="bioC"
                     /locus_tag="ACHKCL_001731"
     CDS             complement(20055..20810)
                     /gene="bioC"
                     /locus_tag="ACHKCL_001731"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5762719.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20797..21951)
                     /gene="bioF"
                     /locus_tag="ACHKCL_001732"
     CDS             complement(20797..21951)
                     /gene="bioF"
                     /locus_tag="ACHKCL_001732"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5762720.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21948..22988)
                     /gene="bioB"
                     /locus_tag="ACHKCL_001733"
     CDS             complement(21948..22988)
                     /gene="bioB"
                     /locus_tag="ACHKCL_001733"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5762721.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23075..24364
                     /gene="bioA"
                     /locus_tag="ACHKCL_001734"
     CDS             23075..24364
                     /gene="bioA"
                     /locus_tag="ACHKCL_001734"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5762722.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24423..24659
                     /locus_tag="ACHKCL_001735"
                     /pseudo
     CDS             24423..24659
                     /locus_tag="ACHKCL_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            24714..24806
                     /locus_tag="ACHKCL_001736"
                     /pseudo
     CDS             24714..24806
                     /locus_tag="ACHKCL_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24903..25601)
                     /gene="nleD"
                     /locus_tag="ACHKCL_001737"
     CDS             complement(24903..25601)
                     /gene="nleD"
                     /locus_tag="ACHKCL_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO5762723.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(25663..25784)
                     /locus_tag="ACHKCL_001738"
                     /pseudo
     CDS             complement(25663..25784)
                     /locus_tag="ACHKCL_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(25832..26713)
                     /gene="nleH1-1"
                     /locus_tag="ACHKCL_001739"
     CDS             complement(25832..26713)
                     /gene="nleH1-1"
                     /locus_tag="ACHKCL_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO5762724.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(26883..27038)
                     /locus_tag="ACHKCL_001740"
                     /pseudo
     CDS             complement(26883..27038)
                     /locus_tag="ACHKCL_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            27317..27421
                     /locus_tag="ACHKCL_001741"
                     /pseudo
     CDS             27317..27421
                     /locus_tag="ACHKCL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(27541..>28536)
                     /locus_tag="ACHKCL_001742"
     CDS             complement(27541..>28536)
                     /locus_tag="ACHKCL_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
                     /protein_id="HFO5762725.1"
                     /translation="NMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYVLDIGKRIP
                     LSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNE
                     KVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEAGENDNEEP
                     YVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEILARRVAQEL
                     GWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISDRYEASPDF
                     TEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVDKHNQQ"
BASE COUNT         6812 a         7539 c         7198 g         6987 t
ORIGIN      
        1 aaacaataaa atcagacatg tcatgcatgt ctgaccgtgt tacctgaata atgggcaaaa
       61 gcctgtgata ctcttttgcc cttacatggt attactgcgc agcggcaggt ttacgcggac
      121 ggcgacggcg ttgttgttcg cctgccggtt tggcatcgcc aagacgacga gatggttttt
      181 ctgcaggctt cgcgctagct gatttcgcac caccttctcc acggcgtggt tgctgttgac
      241 cgcgaccacc accaccttgc ccacgaccgc cgccgccacg ttgctggcga ccgttctgga
      301 tcggttcggc tttgattgac gggtccggct cataacccgg aatcgcaatg cgcgggatct
      361 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
      421 acaatgcttc gccagttgcc gccgcacggc cagtacgccc gatacggtgg acataatctt
      481 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
      541 gcgcagcgat gtcagttgcc accagtacac gaatatcgcc cgatttaaaa tcggccagcg
      601 cacgggtacg cgcaccttgc gatttattac cgtggatcgc cgcactacgg atgccatctt
      661 tattaagctg ttcagcgaga tggttagcgc catgtttggt acgggtaaac accagcacct
      721 gctgccagtt ccctttccca atcatgtgcg acagcaattc gcgcttgcgt ttcttatcaa
      781 caaagtgaac atgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttctattt
      841 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcgg
      901 agaacaacag gttctggcgc ttcgcaggta gttttgttaa cacgcgacgg atatcgtgga
      961 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
     1021 gcttcactgc attctgatgt tccaggtcca gcaaacgtcc cggcgttgcc accagcacat
     1081 caacgccgcc gcgcaacttc atcatttgtg ggttaatgct gacaccgcca aacaccacca
     1141 gcgaacgaat gttcaggtat ttgctgtaat cacggacgtt ttcgccaatc tgcgccgcca
     1201 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
     1261 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgttttg ccggtgccgg
     1321 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
     1381 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
     1441 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
     1501 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
     1561 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
     1621 catactggac aactcccata tggagtcata atcttaatca atcatttgat taagaatgga
     1681 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
     1741 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
     1801 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
     1861 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcatgc
     1921 cgaggaagcc gaacgcttgt tcgcccaacc acagcctgat cgggctgcca tccgtgaact
     1981 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
     2041 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctaccacc tggtgcacga
     2101 acaggtgatt agtccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
     2161 cgacgccaat gacacccgca tgatccttca tacccatgcg ttgattggtg agattctggc
     2221 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
     2281 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
     2341 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta
     2401 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
     2461 acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt tggggggcgc
     2521 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
     2581 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggcgt ctcggtggca
     2641 caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
     2701 gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta taaccgccag
     2761 caaggactgt ggaaaagccg caccatttcg gcaaatgacc tggaaaatgc ccgctcctcg
     2821 cgcgaccagg cacaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
     2881 ggtaaccgtg aacaggacat cgctcaggcg aaagccagcc tcgaacaggc gcaggcgcaa
     2941 ctggcgcagg cggagctgaa tttacaggac tcaacgttga tagcaccgtc tgatggcacg
     3001 ctgttaacgc gcgcggtgga gccaggcacg gttctcaatg aaggtggcac ggtgtttacc
     3061 gtttcactaa cgcgtccggt gtgggtgcgt gcttatgttg atgaacggaa tcttgaccag
     3121 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccctatcac
     3181 gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
     3241 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
     3301 ttacgccagg gaatgccagt gacggtacaa ttcggtgacg aggcaggaca tgaatgatgc
     3361 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
     3421 gccgctcgat tgcaccattc atgccggtta tgtgacgggg ctggtagggc cagacggtgc
     3481 gggtaaaacc acgctgatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
     3541 cacggtgatt ggctttgatc cgatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
     3601 tatgccgcag aaattcggcc tgtatgaaga tctgacggtg atggagaacc tcaatctgta
     3661 cgcggatttg cgcagcgtca ccggcgaggc acggaagcag acttttgctc gcctgctgga
     3721 gtttacgtct cttggcccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
     3781 acaaaaactc ggcctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
     3841 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
     3901 ggcgggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
     3961 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggagaac caaaagccct
     4021 gacacaaacc atggccggac gcagctttct gatgaccagt ccacacgagg gcaaccgcaa
     4081 actgttgcaa cgcgccttga aactgccgca ggtcagcgac ggcatgattc aggggaaatc
     4141 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgacgggat
     4201 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcgttta ttgatttgct
     4261 gggcggtgcc ggaacctcag aatcgccgct gggtgcaata ttgcatacgg tagaaggcac
     4321 acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg attttgccgc
     4381 caccgatcac gtcaactttg ccgttaaacg cggggagatt tttggtttgc tggggccaaa
     4441 cggcgcgggc aaatcgacca cctttaagat gatgtgcggt ttgctggtgc cgacttccgg
     4501 ccaggcgctg gtgctgggga tggatctgaa agagagttcc ggtaaagcgc gccagcatct
     4561 cggctatatg gcgcaaaaat tttcgctcta cggcaacctg acggtcgaac agaatttacg
     4621 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
     4681 gagcgaggca tttggcctga aaagtattgc ctcacacgcc accgatgaac tgccattagg
     4741 ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggata ttctgtttct
     4801 cgacgaaccg acttccggcg ttgaccccct cacccgccgt gaattctggc tgcacatcaa
     4861 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcgga
     4921 atattgcgac cgcatcggcc tggtgtaccg cgggaaatta atcgccagcg gcacgccgga
     4981 cgatttgaaa gcacagtcgg ctaacgatga gcaacccgat cccaccatgg agcaagcctt
     5041 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctttcct
     5101 ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca gatcgttcgc gatccgagta
     5161 gctggctgat tgcggtagtg atcccgctgt tgctgctgtt tatttttggt tatggcatta
     5221 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcagcgc
     5281 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
     5341 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattcccg
     5401 tggatttcgc cgaacagatg gagcgcgcca acgctaccgc accgattcag gtgatcaccg
     5461 acggcagtga gccgaatacc gctaactttg tgcagggata tgtcgaaggg atctggcaga
     5521 tctggcaaat gcagcgggcg gaggataacg ggcagacctt tgagccgctt attgatgtac
     5581 aaacccgcta ctggtttaac ccggcggcga ttagccagca ctttattatc cccggtgcgg
     5641 tgaccatcat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
     5701 gggaacgcgg cactatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
     5761 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
     5821 tgtcagtgtt tattctcggc gtgccgtatc gcggttcgct gctgattctg ttttttatct
     5881 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
     5941 accagttcaa tgccgctcag gtcgccctga acgccgcgtt cctgccgtcg attatgcttt
     6001 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
     6061 ttcccgcacg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
     6121 tggtgctggt ggtgaacgta ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
     6181 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
     6241 acgttaatcc gcaaagagtt gcagtcgttg ctgcgcgaac cacaaacccg cgcgattctg
     6301 attttaccgg tgctgattca ggtgattctg ttcccgttcg ccgccacgct ggaagtgacc
     6361 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
     6421 cgttttgccc gcgccagcgc ctttacacat gtgctgctgc tgaaaagccc acaggagatc
     6481 cgcccgacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
     6541 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
     6601 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
     6661 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
     6721 ccgaatctcg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatt
     6781 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
     6841 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
     6901 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
     6961 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta tggtttatcg
     7021 ctggtgggat tcggtctgtt gatttcatca ctctgttcaa cacaacagca ggcgtttatc
     7081 ggcgtgtttg tctttatgat gcccgccatt ctcctttccg gttacgtttc gccggtggaa
     7141 aacatgccag tatggctgca aaacctgacg tggattaatc ctattcgcca ctttacggac
     7201 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
     7261 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
     7321 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
     7381 cagccctgca attgccagca aaaagttgtt ttccagtacg cgggacaaca gccacagcac
     7441 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
     7501 acgccagcgt tgctcggggt ggcgctgaaa caccagcatc atcactaccg tgacacaata
     7561 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
     7621 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagacccg ttgcgacacg
     7681 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
     7741 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
     7801 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
     7861 ttcaaggtgc tcaccatcaa tattcacaaa ggctttaccg cgtttaaccg acgcttcatt
     7921 ttgccggaac ttcgcgatgc cgtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
     7981 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
     8041 tacgagtttc tcgccgacac tatgtggagc gattttgcct acggtcgcaa tgccgtatac
     8101 ccggaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
     8161 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgccg
     8221 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
     8281 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
     8341 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
     8401 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
     8461 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
     8521 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
     8581 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
     8641 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
     8701 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca actgcatgcg gcactactgg
     8761 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
     8821 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt agtgttccgc tactacgatc
     8881 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
     8941 tggtaatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
     9001 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
     9061 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
     9121 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
     9181 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
     9241 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
     9301 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
     9361 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
     9421 gcggcgttca ggtgtttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
     9481 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
     9541 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
     9601 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
     9661 ggtggaacct aaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
     9721 ttggctggct tccggcacac acgccacgtc tggcgcaggt tgatcctccc gcacaaccga
     9781 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
     9841 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
     9901 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
     9961 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    10021 gatttacggc tgctatgacc tgctcgccca cttttactgc ggtcacaaac tggcgaagcg
    10081 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    10141 cggcggcatt ggtatgcgct atcgtttgta ctcccgtctg ggattgccgg gcagcactat
    10201 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    10261 gattatcttt accgcaggcg tggtggagtt gccggaccac tggtatgtcg atcaaactac
    10321 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    10381 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcgtggaa
    10441 attcgccctc gcccagatgc tgatttccag cgttaactgg atggtcatgg gggcgattat
    10501 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tgctggttag
    10561 cagtattgcc ggcgtcatcg tgcatgttcc ggcggggatt ggtgtgctgg aagcggtgtt
    10621 tatcgcgcta ctggctgggg agcatacatc caaaggcaca attatcgccg ccctactcgc
    10681 ttaccgtgtg ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgttgct
    10741 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    10801 gttctttagt atgtgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    10861 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    10921 tgcacctgta aaagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    10981 caattcaagg agcttatttc gtccatgcaa gataatgcct gaccataaca caatcattaa
    11041 gcaacttatt acccaaatgg gccgttcact cattaatgcg gcattcacga tgattaccag
    11101 ggccaaccct gtcaacgtca tcataatcat aaaacggtga gaaccaggat caatattaaa
    11161 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    11221 aatggcatat acaggaacta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    11281 atatattacg cctgcgatgg cggcatttat cttgttgata tccttaatta agccatacat
    11341 acaaagtaat aataacccta tggcgacata cattggtggt gaatcatatt tggttgccgt
    11401 aatttcgaga tacttagccc cccaggccag ccctgcggtt gccaccaaac ctaaagcggt
    11461 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    11521 ttgcaagtat gattccattc ttttagttgt ccttatagaa aaatattaat attcatcatg
    11581 aataaattta aacccacgca tacaaaatac ttttgtattt atggatacgg aaaactcttc
    11641 cgctcggcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt ttagtaagaa
    11701 aaaaaatccc gacacatggc cgggatattg atggtgaaaa aagaattaac gacgattgcc
    11761 aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg ttaatgcgca
    11821 aagaatggaa tatttgcgca ggttcgacgc gtcgcgggta tctatctgct cacccatatt
    11881 tttcagtttc tgggtgtcat acgccgtcaa tccgacaaag acaatcacgc cgatgtaggt
    11941 gactgcccac atcaacgctt cgcttttcag ccagaagttg accagcgatg ccaacacaat
    12001 gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct tcgtggtgta
    12061 accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac tggcgataga
    12121 agcagcggta taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag
    12181 cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat
    12241 aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag tgaacaacag
    12301 ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaataagcc
    12361 aacggtcatc cagccataga cttgcgccat ataagtctgc aagccagccc ggggttgtac
    12421 aattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct
    12481 taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc
    12541 ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaactacc agcgttttgc
    12601 cgcctgctga tcgctctccc gagcttcgac ccagcggtcg ccctccggcg tggcttcgcg
    12661 cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg cttcaaacgc
    12721 actgctgcga tgcgcactgg tgacaccgat aaaaacgatt tcatcgcccg gccataattc
    12781 cccgatgcgg tgaatcaccg tgacgagccc cagcggccag cggttacgcg cttcatcgac
    12841 aatttctgcc agtgcttttt cagtcatccc cggatagtgt tcgagggtta atgctttgac
    12901 gctgtcgccc aggttatggt tgcgcacctt accggtaaag gtgactaccg caccgtcttc
    12961 gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg gaccaacaac
    13021 aattttggtt tctgccatct taacctccgg ttaccggcgg gaagaaagct acttcgtcgc
    13081 cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc agtaatttgc
    13141 catcttccag cgccagcgcc cagcgatcgc tctgcgcagc caggtgctgg cgtaacgctt
    13201 caacggttgg gaaatccgca gccacttcgg ttgcatctgt tcctaccaac tcgcgcacct
    13261 gggcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga cttgccgccg
    13321 ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc tttgcacatg
    13381 tcataaatgg tcagcgccgc cacggaggcc gcggttaacg cttccatttc gacaccggtt
    13441 ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg ctcggcctgt
    13501 aaattgactt caactttgct gagcatcagc ggatgacaca gcgggatcag atcccaggtg
    13561 cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg
    13621 cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc
    13681 gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc gccagcggcg
    13741 ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa ttacacggac
    13801 gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg cacgctttgg
    13861 tcgaacccgg catggcgaaa atcagcgttt tgttagcgac gcccgctacc gcacgagatt
    13921 gcaacgtgga agtgccaatc tcttcaaacg acaacatacg gaacacttca ccaaaacctt
    13981 caacttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc
    14041 cagtcccccc cgtaatcaac accacttgta catcgtcgct ggcgatccac gcagatacct
    14101 gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg tgatggcccg
    14161 cttcttgcgc cgaatcgcgc aaatagtgac cggaggtatc gtcttcttca ccgcgacgat
    14221 tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc tgactcatct
    14281 gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta ataccggtgt
    14341 tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa atacgcgctt
    14401 ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg ccgccttcac
    14461 caaacaggca gagatggagt ttaccaatgg aggaaacgcg cagacggttg caagtggcgc
    14521 agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatcc ggatgacaaa
    14581 agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg cgacgcagta
    14641 gctcgtcacg cagaacctga ccagagatgt gatgcttacg gaaaagctcg ctgccctcgc
    14701 ccgtttccat cagttcgatg aaacgcagct ggatagggcg atgctggatc cagttcagaa
    14761 aggtgtcgag ctggtgatga ttcacatcac gcatcagcac ggtattgact ttgaccttct
    14821 caaaaccggc ctcaaatgca gcatcgatcc ctgccatcac ctgattgaat ttatcctggc
    14881 cggtaatggc atgaaactgg cgggcgtcca gactgtcgac gctgacgtta atgccagtaa
    14941 gtcccgcatc gcgccagttc gccacatcgc gttccagacg gtaaccattg gtggtgaccg
    15001 ctatctggcg aatagcgtcg ttttctcgca cagcggcgat gatatcggta aagtcgcggc
    15061 gtaaagacgg ctcaccgcct gtcaggcgca ctttttcggt gcccagactg gcgaaggcgc
    15121 gcgtaacccg gcgaatttca tcgacggtca gaaagccttt attggtgacg ccgctcggtt
    15181 tgtagccatc cggcaggcag taggtgcaac gaaagttaca cacatcggta atcgacaggc
    15241 gcaagtagta aaacttacgc gcaaatgcat cagtcagttg tgaagccatg tacacctttc
    15301 cagatacggg aggcgaagtc atttcttcct tcgccctggt ggcaatcttt acgagcattg
    15361 tcacggccaa agcaccatat cagttgaccc aggtgcagag gctagagtgt ttagtggtta
    15421 agccgatact agcgtgtaaa tgagattttt accatccaca ctttcgctat gtaatcatgt
    15481 atatagcgtc ctgatcgtgc tatttcgcta cagaataagg gaaaatggcg gttttacttt
    15541 gatatacatc atcagggagg catagcgtgc atcgtccttt agaggtagaa gaaaatagtt
    15601 tgtatcctca atattggcag gttaattgct gtttccccgc aatttgcgct actgtagcgc
    15661 ggataatctg actccaggaa actatatgcg caatcgtacg ctggctgatc ttgatcgtgt
    15721 cgttgctctc ggcggagggc atggactggg acgcgttctc tcatcacttt cgtctttggg
    15781 ttctcgttta acgggtatcg tcaccaccac ggataatggt ggctcgacgg ggcgtattcg
    15841 ccgttcagaa ggcggcattg cctggggcga tatgcgcaac tgccttaacc agctgataac
    15901 tgaacccagc gtcgcttcag caatgtttga atatcgtttc ggcggtaatg gcgaactttc
    15961 cgggcacaac cttggaaacc tgatgttgaa ggcactggat caccttagcg tgcgacctct
    16021 tgaagcgatc aatctgattc gaaatctgct gaaagtggac gcacatttga ttccgatgtc
    16081 ggagcatcct gttgatctga tggcgattga cgatcagggg catgaagttt acggcgaggt
    16141 caatatcgac cagttaacta cgccgattca agagttattg ttaacgccta atgtacccgc
    16201 aacgcgtgag gcggttcacg ctatcaatga agcggatctc atcattattg ggcctggcag
    16261 tttttacacc agcctgatgc caattctgct gctgaaggaa atcgcgcagg cattacgccg
    16321 cacgccagcg ccgatggtct atatcggcaa tctggggcgt gagttgagtt tacctgcggc
    16381 caatttgaag ctggaaagca agctggcaat tatggagcag tatgttggta aaaaggtcat
    16441 tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc ggattgtgat
    16501 tcaggaggtg ctggaggcca gcgatattcc gtatcgtcat gaccgccagt tgttacatag
    16561 cgcactggaa aaggcgttac aggctttagg ttaacggaaa tccgaaggaa aattccggct
    16621 tcctaatgaa gacaaagtgc gcgttgttta tgacggatgc ggcgtgaacg ccttatccgg
    16681 cctacaaacc gcgctaattc aatgtattgc agaatcatgt aggcctgata agcgtagcac
    16741 atcaggcaat tttgcttttg tcatctgtct tcttcgttat cctgtcacga agccgcgata
    16801 aacaattcac gcagctgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga
    16861 ttttgcgcgt gttgcatcat taacccttcc agctcatgga ttttctgttg caacgcttta
    16921 ggcgacatat ccatcggcac attatccggc tcaacaatcg gtcgcgattt tcctctgccc
    16981 ttcgctttgg ttttagcaat gttctgcccc aacgccagga tatcgaccac tttcttgttc
    17041 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt
    17101 tcggtttcgc caatcgcttt cgccattgac ggggtgatct tatcgccgta gagaatcgct
    17161 ttaccgttaa cgttacgcgc cgcacgacca atagtctgga tcaacgaacg ttcagaacgc
    17221 aggaagcctt ctttgtcggc gtcgaggatc gccaccagcg aaacctccgg catatccaga
    17281 ccttcgcgca gtaagttgat ccccaccagc acgtcgaact cgcccagacg caagtcgcgg
    17341 ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcaccg
    17401 tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt ggtgaccagt
    17461 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
    17521 gccactggcc gcacttcgat aatcggatca agcaatccgg ttggacgcac cacctgatcc
    17581 accacatcgc cgccggattt ttccagctca taattacctg gcgtcgccga aacatagatg
    17641 gtttgcggcg ctaatgcttc gaactcttca aatttcagcg gacggttatc cagcgctgat
    17701 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
    17761 ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcggcaggc
    17821 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
    17881 tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta
    17941 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttgcgtctg
    18001 gcggccagct cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg
    18061 tagtgcgttt tcgggtagat ggtaaaacgt ggaatggtgg aaacaatctg cccggttagc
    18121 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg
    18181 tcatccgatt ctgccgggaa gatatcaatc acctcgccac gaacacggaa agtaccacgc
    18241 tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc
    18301 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
    18361 cccagaccat aaatcgcgga aacagacgcg accacaacaa catcacgccg ctccagcatc
    18421 gctttggtgg cggacaaacg catctgttcg atatgttcgt ttaccgaggc atctttctca
    18481 atgaaggtgt cggaactcgg tacataggct tctggctgat agtagtcgta gtaggagacg
    18541 aaatattcca ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc
    18601 agcgttttgt tgggcgcaag taccatggta gggcgctgaa ggtcagcaat gacattggca
    18661 atggtgaagg ttttcccgga accagtcaca ccaagtaacg tctggtgcgc caggccatct
    18721 tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg
    18781 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
    18841 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
    18901 catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgag taaaatttac
    18961 tctgtggcga gataaaactc ggaccttacc gggttatccc caaagcaacg gcttttttaa
    19021 catttgtcaa gatgagtgat gtcagtttta tggcaagaga tgcctgttca gtgactgcca
    19081 ttgcaattat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
    19141 attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
    19201 ggttatccac aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa
    19261 ttcccacatt cgccgcatga tgcggaagta aaattttttt cgtgattatt ttagaattta
    19321 attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacagg
    19381 atggctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg
    19441 caagccaggg gctctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
    19501 attcagcgtg acgttttccc ggaggcgtga catcgttcgc cacccaaccc gccagagtca
    19561 gcccggcgtg ttgtatcgcc tgtgccgtca acatcgcgtg attaatacag ccaagtttca
    19621 cgccaaccac cagtattacc ggcagttgtt cctgtgttac ccaatccgca aaagtgaaag
    19681 tgtcagaaag cggcgtaaac cagccgccag cgccttccac taacacccag tcagcctgtt
    19741 gttcaagcgc gcgtaatccg gagctcatta ccgatgattc tatcgacctg ccctcttgcg
    19801 cgctgatgat gtgcggtgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat
    19861 ccagctgcag gctgctgttg cgctgtaacg ccagcgcgtc actattacgt aaaccctccg
    19921 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgatag cctgctgcct
    19981 ttgcggcttg taaaagtgca caactggcga cagttttccc cacttcggta tccgttccgg
    20041 tgacaaaata acgtttactc acgaacaatc actcccaaaa aaagatgata cgtcagagga
    20101 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaactgtga acgcgttaat
    20161 attcgctggt cgcgcccttc atgaagatgc gtggcaccga tgcctttcag cgaacgcatg
    20221 gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tacgatgttg ataatgcacg
    20281 ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg cgggcgctcg
    20341 tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
    20401 gcgaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt tgataaatta
    20461 ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
    20521 agggattcga tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcgca
    20581 agcattggcg gcgagagatc taacgccgtc acctgcgcgt gacgctctcg ccagtggcgg
    20641 ctcatccagc caggcccaca ccccgcgtcc agtacgtggg ggtatttacg ctgtgggagc
    20701 attgccagta agacatcagc actctggcgc tgtaactctg cgtgttgctc atagtgcgcg
    20761 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
    20821 caacagacgg tcgatatcct gcatttcatg cgcggcggtt agcgttaagc gcagtcgcgc
    20881 agtaccagag ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
    20941 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
    21001 gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgtaatgag
    21061 tgccgccagt ttttcgcgcc gtgcatcacc ctcatcactg cgaatgaccg ccagtgacgc
    21121 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataagat ggcgggcgaa
    21181 ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccgc tgacgccaaa
    21241 tcctttgcca aaagtcacta ccagcaattc tggttttacc ttttgcaacc agcaggtgcc
    21301 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
    21361 gtgctgttgc gttacctgct ggatttccgc cagtggcgca ctatcgccgt ccatgctgaa
    21421 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
    21481 atgagtgaca tcgttatgaa caaaacggcg aagctgcgac gggcttaaac tggcagcctc
    21541 cagcaatgag gcatggctaa gccggtcagc gacaatgcgg tcctcttttg ccatcatcgc
    21601 ggcaatgacc gcctgattag cggcaaaacc agagataaat agcagtgccc gcgaatagcc
    21661 cagccactcg gccagttctt cttccagcgc ctgatgcgca acgctataac cgctgacgtg
    21721 accggaaccg ccgctaccga cgccaaattg ctccgcaccc tgcttccagg cacggataat
    21781 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    21841 gtccgccacc agccagcgtc cggctccttg cgccactgga taacggcgac gcagggcatc
    21901 ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc
    21961 gcgttgtaat attcgtcagt gtccggggtc atcagtgcct gctccagacg ctgctgttgt
    22021 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcgaaacagt
    22081 tgcaggtctt tatcttcttc cggattgggc gtggttagca gtttgcaacc gtagaaaatc
    22141 gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg
    22201 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat ggtgcgaata
    22261 aaatcaaagg catcgacatc atcgttatct gccagtggag tacctttcac cttcaccagc
    22321 atgttgattg gcacgctttc cggcggcgtc ggcaggtttg ccagttgcag taataatccg
    22381 gcgcgatctt ttaccgtttc gcctaaaccc acaatgccac ctgagcagac tttgatcccg
    22441 gcatcgcgca ctttttccag cgtatcaagg cgttcctgat aagtacgtgt ggtgatgata
    22501 ttgccgtaaa actccggcga ggtgtcgagg ttatggttgt agtaatccag cccggcattc
    22561 gcaaggcgct gcgcctgaga ttcactcaac gtacccagcg tcatacacgc ctccagcccc
    22621 atcgctttta ctccctgcac catttgttcc aggtatgaca tatcgcgttc gtggggattc
    22681 ttccacgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac
    22741 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag
    22801 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaaggtg
    22861 ctgacctgca cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc ttcaaacagc
    22921 agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg
    22981 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa
    23041 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    23101 ccaacgccat atctggcacc catacacatc catgacctcc ccgctaccgg tttatccggt
    23161 ggcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat
    23221 gtcgtcctgg tgggcggcga tccacggtta caatcacccg cagcttaatg cggcgatgaa
    23281 gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgccagccat
    23341 tgagctgtgc cgcaaactgg tggcaatgac gccgcaaccg ctggaatgcg tttttctcgc
    23401 ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaagc
    23461 caaaggcgaa gcgcgccagc attttctgac cttccgcaat ggttatcatg gcgatacctt
    23521 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    23581 gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgagcg
    23641 cgatatggtg ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cagcggtgat
    23701 cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    23761 acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    23821 cggatttggt cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggacat
    23881 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac
    23941 gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc tgctttatgc acgggccaac
    24001 ttttatgggc aatccgctgg cctgtgcggc tgcaaacgcc agcctggcga ttctcgaatc
    24061 tggcgactgg cagcaacagg tggcggctat tgaagtacag ctgcgcgagc aacttgcccc
    24121 cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg ggggccattg gcgtagtcga
    24181 aacaactcgt ccggtgaata tggcggcatt gcaaaaattc tttgtcgaac agggtgtctg
    24241 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    24301 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    24361 ataacgggaa gtcagcgtga gagtttctgg ctacactttc tgcaaactag aaaggagggt
    24421 ttatgaaact catcagtaac gatctgcgcg atggcgataa gttgccgcat cgtcatgtct
    24481 ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc
    24541 ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg
    24601 gctggtggca ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaaggtg
    24661 atttttgtct tcttgcgcta attttttgtc aaacacatta cgtcgtttgc tgaatttaca
    24721 accatgctca gtatctctat aagcgcagag aaatgatgca gtggtgggtg ggctggcttg
    24781 atgaaaaggt ggggtactcc acctgaacca ctatcggaat agcacaaagc cttgcaatcc
    24841 agtgcaaagt tttgtgtgcc tccgtttgcc ccaccaatat atattcagca caagaaacac
    24901 agctaaagca atggatgcag tcttacctgt tggaatccca gactcactgt attgtcatca
    24961 tgaataagag ctgagtcgtg cgggtaggag gttctacggg gcatcccaat ctcttcgtgg
    25021 aatttatttt ctgaaagcac ctcctctgaa aaagccccca acccaacagt cctggcttct
    25081 tcgagtaaaa gtggagagta tttttgtgat tctgctcggg aactctcaac tttcaaacgc
    25141 tccccattta aattgtggaa aacatggagc aactcatgaa aaacaatagt cgcatgaaag
    25201 tccaccacgc taatcccctc cccacaggga tattcaactg cattcagatt acagtgaaaa
    25261 tcggaaccag tcccccgatg gttctcagca tctatatctc tatgtgccat aactcctagt
    25321 ctggaagagt tgaggtgtat taccactgtt tctgatttaa gtcgggatat tgattcaata
    25381 gcattcagga gtacacgccc tgtctctgta gagccaatct tatcgagtgc tgcttcaatc
    25441 tgctgatata tattatgatc agtcccctgg aaacgaagtt gcggattatt ttttatccat
    25501 tgaacgctcg atgcttttac aagagactcg atatctgtat ctgacattga gctcgacctt
    25561 gatgactgat gtaataccaa gttgagggac gtagggcgca tcaggactcc tgagaatgct
    25621 acttattagc agcctgttta ccactctgtg aaaatgattt ttttaaaagt gaccgtcaag
    25681 gcggtcacca aacccgataa taaagcggat catagccatt tgccagtcct cagcgacatt
    25741 gcccatttcc gtgatccgcc aagaattgcc aaccacacaa tttgttacca actccatcca
    25801 tgcaacaaat ccactacact ggataaaatt actaaatttt acttaatacc acactaataa
    25861 gatcttgctt tccgccatga taagattgca ttatttgact ttcactccat gaacggtcgg
    25921 aaacattata agatgatata tctattggat taaactcatt cttcgcgcgg tcatataaga
    25981 catttgtctc tgttgtatcg acaaaaagaa ttcctttttg ctccagtcta tcaaacatat
    26041 cgtaaatagc atgctcagcc tgtgctggca aggacgaaat atttaaaagc gattctccat
    26101 ttattttatc cattctaata ccaataatat caccattatt gccatatatt ttttctgcac
    26161 tcccggcacc ataatattgg ttgaagcaac gaacttcgct tgtcacctct tcattgcttt
    26221 gagatgtagt aaacatcttc aggacttttg ttgcatcctc agcatcttca tatactacag
    26281 cgttcccccc tttgccaata acattaccag gtactgacaa cctgttatag tccacactcg
    26341 gtaactctgg cggtgcataa tcaacaggag gtaaatcagg tctgttcgaa tgaacaatgc
    26401 ctctttccat agcagcgctc actggtgatg cattcagcat gacctcaatt ttcctgctaa
    26461 tctccccctt aggccatccc agcctgtgca acagattagt aaaacaacca ctatgacttt
    26521 ctcttgtaac gcaaaactta ttatcggtgg caacaacacg atatgttctg ttgccaacct
    26581 ttacttgcgc cccattatca gaatgaacgg cagcatccct tacagaggat aaaacacgat
    26641 tatcaggcga agtcaggttt ctggttaaag aattccatga acatcccaaa tttacagaat
    26701 atggcgataa catacatttc aaccttcaaa ataaacccat ctaaatttcc ttaacaaaca
    26761 tcccccctaa catggcaaca aaaattagaa atgaactctt gctttttgta tctggcaact
    26821 aattcatcct tccagcatcc catcaaacga acgtttacga actataacgc aggtattacc
    26881 ggtcactcga tatctggaga tacagacgta tcaacatgat tgagcggcat acgatactgc
    26941 ttccatgccg ccagtaacga tgtctgctca tccgtcgcaa tatctaaatc tgcagcatcc
    27001 tgaagtggtg cgatatactc actgattaca agtaccagac cgatagatta aaaaaaacac
    27061 aataacacgc tgcatttaaa acaaaaaatt ttaaatatga tttaatacaa gctttaagcc
    27121 tttaattatc agttttattt attttccagc aattgattaa tcaattgttt atatacaaat
    27181 tattcctttt ctttctctca cgcaaaattc acaaaatatt gttgctgcaa tggacctgta
    27241 caatagtgaa tcacggtact tttctgtgca caacgtactg cagtttcttt acgatttata
    27301 gctgtactgt tgaattatgt acaatttgaa tccatgcatg acgtgtgatg cctattgtgc
    27361 atttttccga gcctcttttt actgggccga agccgatgat gctgatagtc tcgttccggc
    27421 catgttcact tactacagta aaattagggg aattgccttt acatacaatg ttgcacgatt
    27481 acccatcatc aactccgtta aaatgtatga atagtaacct tatgtcactg caaagacgaa
    27541 tcattgctga ttgtgtttgt ccacatcccc aaatgtaaaa atataagggg cgccatgata
    27601 cagttggatc tgatttgcat cctgtaaatt atcgttgata gccaatccag gaaaatcatg
    27661 ttgctggata aatccgtatc ctatgttaga cacagcggaa tactctgtga aatcaggact
    27721 cgcctcatat cggtcactga tcgtacccag cctttcgaag aaagccctct cattctcttc
    27781 atgcctcatg gcagcctgtc gaagggcatt caggttacgt agcctaagat gagcttcacg
    27841 ttctggctct gcatatcctt tgaagtcggg aacactccat cccagttctt gagcgacacg
    27901 acgtgcgaga atctcggtgg gtcctaactc tatattacta tctccagatg gatcgctaga
    27961 tccagtaaca tgatgaataa tctcgtgaat cagtccttcc tgccacgatg gcatctcata
    28021 agagtcagta tctggcgcaa cactaaaact gacataaggc tcttcgttat cgttttctcc
    28081 tgcttcgcaa atggggaaaa ttggctcttg cccgacatca tattcatata attcgttaca
    28141 ggttagtgat tgaatatcat caattttgac agaagattct tcgttaagct cgtattcgtt
    28201 tctgtattta atgcaaccaa tgtgtacctt ctcattatga atgccatagc ttacggcatc
    28261 acgaaatgtc tgtgatcggc tcaaagcatc aagtacagtg ttgccgatca tatcgactgt
    28321 atgctgatcg ataagcctgc tacggctgtc atggacggcg cgtattacac tttcgtatac
    28381 gttgcttaaa tctgctgcgg aaagtggtat tcgcttacct atatccaaaa cgtaatccgc
    28441 ataggcattt tcagcacgat taggagcaat gggagcagag tatcctgccg gggcggaaaa
    28501 gttgaagttg gactgtaatg agggaatttt catgtt
//
LOCUS       DAZFKY010000045        28376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFKY010000045 DAZFKY010000000
VERSION     DAZFKY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..129)
                     /locus_tag="ACHKCL_001743"
     CDS             complement(<1..129)
                     /locus_tag="ACHKCL_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762726.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYI"
     gene            complement(275..4177)
                     /gene="hrpA"
                     /locus_tag="ACHKCL_001744"
     CDS             complement(275..4177)
                     /gene="hrpA"
                     /locus_tag="ACHKCL_001744"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5762727.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4378..4983
                     /gene="azoR"
                     /locus_tag="ACHKCL_001745"
     CDS             4378..4983
                     /gene="azoR"
                     /locus_tag="ACHKCL_001745"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5762728.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5034..6326)
                     /gene="ynbD"
                     /locus_tag="ACHKCL_001746"
     CDS             complement(5034..6326)
                     /gene="ynbD"
                     /locus_tag="ACHKCL_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5762729.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6340..8097)
                     /gene="ynbC"
                     /locus_tag="ACHKCL_001747"
     CDS             complement(6340..8097)
                     /gene="ynbC"
                     /locus_tag="ACHKCL_001747"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5762730.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8113..9009)
                     /gene="ynbB"
                     /locus_tag="ACHKCL_001748"
     CDS             complement(8113..9009)
                     /gene="ynbB"
                     /locus_tag="ACHKCL_001748"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5762731.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9009..9614)
                     /gene="ynbA"
                     /locus_tag="ACHKCL_001749"
     CDS             complement(9009..9614)
                     /gene="ynbA"
                     /locus_tag="ACHKCL_001749"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5762732.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9785..12091)
                     /gene="ydbD"
                     /locus_tag="ACHKCL_001750"
     CDS             complement(9785..12091)
                     /gene="ydbD"
                     /locus_tag="ACHKCL_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO5762733.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12155..13015)
                     /gene="pdxI"
                     /locus_tag="ACHKCL_001751"
     CDS             complement(12155..13015)
                     /gene="pdxI"
                     /locus_tag="ACHKCL_001751"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5762734.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13235..15694)
                     /locus_tag="ACHKCL_001752"
     CDS             complement(13235..15694)
                     /locus_tag="ACHKCL_001752"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5762735.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15914..18892)
                     /locus_tag="ACHKCL_001753"
     CDS             complement(15914..18892)
                     /locus_tag="ACHKCL_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO5762736.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVKHNPVVYKNG
                     VTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSWTVHDDSNTVVFDGVNT
                     SGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQGGNTALNGGTVMTVDG
                     DNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGTAVIINGDNTKLNTAGD
                     STITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGATIKQEGDLYVSGGAHG
                     IDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLNVGQAAAGENAIGIQID
                     GDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFSTGLDVSGNNNNMTLAT
                     YELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPYFFNPSTGVNISGDNND
                     VTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTFTLNGGVHFVGEKNVMD
                     DGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGFENLIQLSHGAELEIGA
                     DATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNIGFAAAWNKDSTVINNG
                     SIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSVLNYPAEWNLSDGTSFN
                     NKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKSTADNEGQITLDTLWVDE
                     NDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHENGVITVYNAGAGMAAY
                     GNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAINDQTGVININAENGQAF
                     YNDGTGLIINYGTICTFGVC"
     gene            complement(19306..19629)
                     /locus_tag="ACHKCL_001754"
     CDS             complement(19306..19629)
                     /locus_tag="ACHKCL_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5762737.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(19637..19822)
                     /gene="ynbE"
                     /locus_tag="ACHKCL_001755"
     CDS             complement(19637..19822)
                     /gene="ynbE"
                     /locus_tag="ACHKCL_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5762738.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(19819..22458)
                     /gene="ydbH"
                     /locus_tag="ACHKCL_001756"
     CDS             complement(19819..22458)
                     /gene="ydbH"
                     /locus_tag="ACHKCL_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5762739.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            22666..23655
                     /gene="ldhA"
                     /locus_tag="ACHKCL_001757"
     CDS             22666..23655
                     /gene="ldhA"
                     /locus_tag="ACHKCL_001757"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5762740.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            23766..24188
                     /gene="hslJ"
                     /locus_tag="ACHKCL_001758"
     CDS             23766..24188
                     /gene="hslJ"
                     /locus_tag="ACHKCL_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5762741.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(24185..24451)
                     /gene="ydbJ"
                     /locus_tag="ACHKCL_001759"
     CDS             complement(24185..24451)
                     /gene="ydbJ"
                     /locus_tag="ACHKCL_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5762742.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            24725..28249
                     /gene="nifJ"
                     /locus_tag="ACHKCL_001760"
     CDS             24725..28249
                     /gene="nifJ"
                     /locus_tag="ACHKCL_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5762743.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSTDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         7170 a         7532 c         6569 g         7105 t
ORIGIN      
        1 aatatacagc gcgcgtcggt aattttcctc tggaagtttt tcatagaaag atattaaagc
       61 ctgaccaacg cgatcaccct ctattatccg ttcaaattgc gacctcagta aaatatcatg
      121 tctactcatt gcaatacgtc ctgttcgccc aaggcgaagt atgaatgaaa cttatgtaaa
      181 atagtaccgc ccttgctata tttcagaatt cataaagaaa aaacccggcg ctaaatgctt
      241 accgggtttt agatttatca ggcaaatagc agggttaacc gctaatctgc tccatcgcct
      301 gcaaaatacg cttatctgaa atcggataag gcgtaccaag ttgttgagcg aagtaactaa
      361 cgcgcagctc ttctatcatc caacggatct ctttcacgtc ttcatcctca cgacgtgcag
      421 gcgggagttt gttgaaccat tgctgccacg cctgctggac gttttcgact ttcagcatct
      481 gtgcacgatc gcgatgtgga tcaaccgcca gtttttccag ccgtttttca atcgcctgca
      541 aataacgcag cgtgtcgccc agccgtttga agccgttacc agtgacaaaa ccgcgatata
      601 ccaacccgcc catctgcgct ttaatgtcag aaagccccag cgccatggtc atatccaccc
      661 gccctttcag acgtttgttg atattgaaca ctgccgtaag gatttgctcg acctgcttcg
      721 caatatccac caccgtgtcg ttcagttcgg cacgcacttt ttcatgcagc gcagcaaagc
      781 cttcttccgt ccagaccggg cctccattgg cgtcgatcag ttgatccaca ccgcatgaga
      841 tacagtcgtc gatcagctcc agcactttgc catacgggtt aaagtacagc cccagcttgg
      901 ctttgtttgg taacttctca tgtaaatact tgattggtga gggaatattc agcagcagta
      961 gacggcgaag accgttccac attgcctgtt tttgctccag cgggttatca aacagtttga
     1021 tcgccacact gtcgcgctca tccaccagcg ccggccacgc cttcactttg tagttaccac
     1081 gcttctgttc gtagctttcc ggcaactgac caaaactcca gatatgtaag ccgctctgct
     1141 cgataccgtc atccgccacc gcagatagcg tttcctgcac tttgcctttc agcgcatctt
     1201 tcagatcttg tagcgagcgc ccttctttta gcttcttgtt tttgtcatcc accacgcgga
     1261 aggtgatttt caggtgatcg ggcacctgat cccagtgcca gtcttcgcgg tcaacggtaa
     1321 cgccggtcat ccgccgtaac tcgcgctcaa ggctgtcgag caacggtaac tccagcggtg
     1381 tgacgcggcc taaaaacgct tcggcatagt ttggcgcagg tacaaaatta cggcgtaccg
     1441 gtttcggcaa cgatttaatc agagcaatca ccagttcgcg gcgcagacca gggatctgcc
     1501 attcaaaccc gctttcctca acctggttaa gtaacggcag cggaatatgt acggtcacac
     1561 cgtcagcatc cgcgccgggc tcaaactgat agctcaaacg cagcttgaga ttgccctgat
     1621 gccagaagtt cgggtaatcc agcttgctga tcttttccgc cccttctttg atcaacatgc
     1681 tcttttcaaa gttgagcaaa tcaggcgttt cgcggctgac ttttttccac cagctgtcga
     1741 agtgacgagc ggagattaca tcgtggccga tgcgctggtc gtagaactca aacagcgttt
     1801 cgtcatcaac cagaatatcg cggcgacgtg atttgtgttc aagctcttcc acttcggccc
     1861 gcagtttcag gttttcacgg aagaatgcgt gacgcgtctg ccagtcacct tccaccagcg
     1921 cgtggcgaat aaagagttca cgacataacg ccggatcgat ctggctgtag ttgaccttgc
     1981 gcgcggcaac aatcggcaaa ccataaacag tgactttttc cgttgccatc accgcgccct
     2041 gcgcccgttc ccagtgcggt tcgctgtagg tacgtttaat caaatgctga gcgacaggct
     2101 ccacccattc cgggtcgata cgcgcagcaa tgcgccccca caggcggctg gtttctacca
     2161 gttccgccac cattacccat ttcggcggtt ttttgaataa accagaaccg gggaagatgg
     2221 agaaacgcgc gttacgtgcg ccggtatatt cttgtttatc ggcatctttc atgccgatat
     2281 gggaaagcaa ccccgtcagc aacgcaatgt gaatttcacg atactccgcc ggttcgctgt
     2341 tcaccggaat gccgagttct ttcaccacct ggcgcaactg ggtgtagata tcctgccatt
     2401 cgcgcacacg cagatagttg agataatcgg tacgacacag gcgacggaag gcgttggaag
     2461 aaagcgcctt ttgctgctcg ccaagataat tccacagatt cacaaacgcg aggaagtcag
     2521 actctttgtc gtggaagcga cgatgttttt cgtccgatgc ctgctgtttg tccatcggac
     2581 gttcgcgcgg atcctgaatg gagagcgcag acgtgataat catcgcctca cgcacgcagc
     2641 catgtttttg cgcttccagc accatacgcg ccagacgtgg gtcgacaggc aactgcgaga
     2701 gctggcgacc gagcggcgtc agtttatagg cgctggcctg ttcatccgtg gtgatcgcgc
     2761 ccagttcttc gagcagacgc acgccatcct ggatattgcg tttatccggt gcttcgacaa
     2821 acgggaacgc agcgatatcg cctagcccca gcgcggtcat ctgcaaaata accgaggcca
     2881 ggttggtacg cagaatctcc ggatcggtaa actccgggcg cgagaggaaa tcgtcttcgg
     2941 aatagagacg aatacagatc ccttcggaca cacgaccaca gcggccttta cgctgattgg
     3001 cagacgcctg cgaaatgggc tcaatcggca aacgctgcac tttggtgcga tagctgtagc
     3061 ggctgatacg cgccgtaccg gggtcgataa cgtatttaat ccccggtaca gtcagcgagg
     3121 tttccgcgac gttggtcgcc agcacaatgc gtcgcccgct gtgcgactgg aacacgcggt
     3181 tctgttcgct attcgaaagc cgcgcataaa gcggcaagat ttcggtatgg cgtaagttca
     3241 gcttgttcag cgcatcggcg gtatcgcgaa tttcccgctc gccgctcata aagatcagaa
     3301 tgtcgccggg gctttcctgg cttagttcgt ccacggcatc gaaaattgcc tgcaactggt
     3361 cgcgctcagt gtcgtcggct tcttcaacaa tcgggcgata gcgcacttcc accggatagg
     3421 tccgaccgga gacttcaata atcggcgcat tattaaagtg gcgcgaaaag cgttccgggt
     3481 cgatagtcgc ggaagtgata atgattttta ggtcaggacg ccgcggcagc aactctttca
     3541 aataaccgag cagaaaatcg atattcaggc tacgttcgtg cgcttcgtca ataatgatag
     3601 tgtcgtactg catcagcagg cggtcttgct ggatctccgc cagcaggata ccgtcggtca
     3661 ttagcttgac catcgtgtta tcacttacgt gatcgctgaa acgcactttg taaccgatgc
     3721 aaccgcccgg ctccgttttc agctcttccg caatacggtt cgctaccgtt cttgccgcca
     3781 ggcgacgcgg ctgggtatgg ccgatcagcc ctttaatccc gcgccccagc tccatacaga
     3841 ttttcggtaa ctgagtcgtt ttaccagaac ccgtttcccc ggcgactatc accacctggt
     3901 gatcacgaat cgcttcgaga atgtcctgtt ttttctgact aaccggcaaa ttgtcaggat
     3961 aagtaatttc cggtcgtgcc gcttcacgca gcaggacttt ccctgccgcc tggtcaatct
     4021 ctttcgccat ctcctggaaa atggcctgtt gtgcatcagg atttttaacc ttcttcacgc
     4081 cgtgcagacg gcgagaaaaa cgcagtctgt cacgcagcat cagcgaatcc agccgctgct
     4141 gcaaggccgt aaaagtcaat ttttgttgtt ctgtcataac gttagtgggc agtagtctgc
     4201 ctgattaatt ttctttgaaa tgatagatat agagtaccac atcgcggctt tctatgcgtt
     4261 gttcaataaa ttcgaacata ggcttcgatt tattgcgcta tctctgtggc aggattgtga
     4321 ataaagtgtc aacaagcaac ggggcatcgc ccattcaaat atctataagg aaacaccatg
     4381 agcaaggtat tagttcttaa atccagcatc ctggcagggt actctcagtc taatcagttg
     4441 tccgattatt ttgttgaaca atggcgcgaa aagcactccg ctgatgaaat caccgttcgc
     4501 gacctggctg caaatccaat tccggtactg gatggcgaac tggttggcgc tctgcgtccg
     4561 agcgatgcgc cgctgactac gcgtcagcag gaagctctgg cactttccga tgagctgatt
     4621 gccgagctga aagcccatga cgttatcgtt attgcggcac cgatgtataa cttcaacatc
     4681 tcgactcagc tgaaaaacta ttttgacctg gttgcacgtg caggcgttac tttccgctat
     4741 accgagaatg gtccggaagg tctggtaacg ggtaaaaaag ccatcgttat taccagccgc
     4801 ggtgggatcc acaaagatgg accaacggac ctggtgacgc cgtatctgtc cacgttcctg
     4861 ggctttatcg gcattaccga tgtgaaattt gtcttcgccg aagggatcgc atacggtccg
     4921 gaaatggcag cgaaagcgca gtctgacgcg aaagcagcca tcgacagtat tgttgctgca
     4981 taaccggaca cgatcttgat gtggactgta gttcagtcca catctcaatc cacttacctg
     5041 ttttcccata atttcaacat cgctttgtgc tcttccttaa gcacaatatg cgagcgtctg
     5101 gctcgaataa agctaatcgc ttcatcaact gttttacagt gtccgtaaca taacagccat
     5161 gccgccacta ccagcgcact gcgcgataat cccaatgcgc aatggaccag aacgctgcct
     5221 tgctcttcgc gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc ctcttccgga
     5281 accaccagat ccagcatcgg tacacagaaa tagagtcgat cttttgtcgc tcgtccgcga
     5341 gggaattcaa aggtgacatc cagaaccgca ttctgtgccg gaatatgtcg tggaaacgcc
     5401 cctaaataaa caccagcagt cattttgctc accggctcca ggcgacgaca aaaccaacgc
     5461 atagatagcc acatcccgat gcgccagggc aatgtcagcc agtaaacggc gggcgggagt
     5521 ttcccctgac tatctttccc tgttgttatc gcgccaagcc caccgtaccc acggccaata
     5581 atgagtagcg ataatactgg ccaacataac cagactgacc accataactg aaccatcatc
     5641 attagctcca tcaacacaat gcacgcgcac gcgcctacga catatggcag tgctattttg
     5701 attcgacgtt gatcaggttt ctgataattc caacgacggt cgacgggtat catccagtca
     5761 atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg ccaggttgtc
     5821 agcatcgaaa tggcgatgag taaaaaccat ccgccgcaga ctttacgcca cctcacagcc
     5881 agatgctgac gaaagtgacg ccaaagtagc cagcagagaa caatatgcag cgaaggagac
     5941 tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt caccccactc
     6001 acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca gcaggccatt
     6061 accgttgcca gaataagccg gtggacaagt cggcgctgtt cgaatgtcga gctacaaacg
     6121 aacagcgaaa atccatataa aagatccaga ctccagtaag ggacaatagt ccagggaaga
     6181 aaagggatcg ccgtttccca gccgaatacc tgactgggga tatcatggct gttaaagtcc
     6241 tgaaccgcgg tgaactgatt tagagatcca taggtgaaga agaaaaacgg ggccagcaac
     6301 aataaccagc cagcgccttg tagcaatacg ttccggcgtt cagttatcac gacgcaccgc
     6361 catcgaaacc gtaaaaatgc cccactcatc aatccgttgt gtgcatttat caaatccggc
     6421 atcacgcacg agtgaatcca tctccccttg cgaacgcacg cgcattaccc acggtttacc
     6481 atctttatga ctggttaaca ccccggcaat cagctccagt tgtgggtgcc acggctgccc
     6541 ggtgtagagg agaatgccgc ccggttcgat ggcattggca agacctgcga gtgagttttt
     6601 tacctgctca tttccgggaa aaagctcata cagtccagag acaatcgcca gcgtaggccg
     6661 cggagttaac gcgctgagtt cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg
     6721 cccagacatt ccccgttgag caatcatctc ttgcccctgt gcaacattta actcgctgta
     6781 atcacgtaac aaaatatcgc ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg
     6841 cccatgccct gcggcaatgt caaccacgcg gacggctaaa cctttggcgt ggagatcggc
     6901 aacggcttgt ttaatcagta tttgtaaatg ggttttgcgc tggcgaatac cgcgccagcc
     6961 aacactgttc aggtagattt tgtcgattaa gcgcccgaat gcgttactac cttgcggttg
     7021 attttgatag acataatcca gcgaactgcc tgaatcaaag ccggtgctca ttccgagatg
     7081 caggcccgca gagtgcgtcc cgaacaattt catcgcttta cgcataaagc gatacgccaa
     7141 atcaaccggc gataatggca cgggtccacc tgaaaggagc cgccagcgat ccgctgatgg
     7201 tccagtgcgg tcttcatgct gataatcaaa tttttgtgac ttgttagcat ataaacgact
     7261 aataaagctt tgcatttttt caaatgcctg cgccctgttc tcttcaccca acgtgtcgtg
     7321 ataaaagcct ggcagcagat gcagctcttt cagagggcta cgtaatctat gataaaaatc
     7381 aatctgcggt tggcgatgca ccacatagtc atcgcctgat atcagaagtt gcgtggggag
     7441 cgtaatcgcc gccgcatcgc taacaatacg ttcagacgtt ttataaagat cgagcaagat
     7501 attaacggca atcgcccgtg tgatcagcgg atcattattg aaactcgcca cccgttgccg
     7561 atcgtgggtc aaatagcgtc ctttcacata ggaattaata aaaaacagac cacgcagacg
     7621 atgccataac gccagcgcag gacgtgccag cggcacatac aatttaacct taaaggccgg
     7681 agaagccagc accagcccgc gaattgcagg cgcataatca tgaacccatg tggcaaccag
     7741 cactgcgccg acgctctgcg cgatcactac cacctcttcc agcccgacct ggctgtcgct
     7801 ggcagcaaaa cggacaaact catcgacatc ccgcactgaa cgcgcaagag atgggctata
     7861 accacggggc cccgaagttt gtccatgccc tcgggcatcc catgcataaa aagcagtatc
     7921 tggcatcgcc agttcatcaa cgatatgttg tagacgacca gaatgttcat gaccgcgatg
     7981 aaataagacg atgacctttt tcgcccctgg ctgtaaagcg ggccagtgcc gataaaacaa
     8041 cgctgtatta tcactggtgg taaaaaaatg ttccccaggg atgcgtgaat tttccatttt
     8101 ctctattttc cttcagtaac agcagtagcg aataaaataa aaaaataccg gagcggtgaa
     8161 aattaatgag tcaatcctgt caaggagtcc gccatgtcct ggtaatagtt ttccgctatc
     8221 tttaacaccc acatctcgtt tgacggctga catcactaca tcgccgcaaa aaccactaat
     8281 accaattaac aaacccgcta ataatgcctg taatgtattt agcggtgtca gcaacggtcc
     8341 aataatcagt gacgcaatca tggtggtgat tacgcccccc accagacctt ccagcgtttt
     8401 tcccgggctg actttgggga ccacttttct tctgccgcag gatttccccc ataaatactg
     8461 tgcaatatcg tttgactctg ttaacgccaa aagaaataac accagtaacg ccccgccctg
     8521 ggtatttgtg gttgtcaaca ttaataacca ggcggcatga ctcaaggcga aaacggtcat
     8581 tatccagccc caaaaaatgg cgctcacggt atgcagaaaa ccggaggtat cacctactaa
     8641 aactcgccag gtggttaata tcagaaaccc agccaaaggt ataaataaca gaaacagctc
     8701 aaagcaacta aaaccgataa gcaaatagtt aagaggaata acccaatata accaacgcgg
     8761 aaaatgtacg gatataagcg tacaatattc tttcaatgcc agaaaactta tcagcgcaaa
     8821 aaacgtcaac gtcatccagc gtggacctga aatcaccagt gaaaaacaaa tgacgatacc
     8881 ccaccaggta cgaatacgta gtgtaacttc atcccctttt ctctccggta accgccatac
     8941 ccgaaaacgg tttatcagcg ttgctaaaat taacaacgcg aaaagtgttg ccagagattt
     9001 ttccagcatt atctttcagc actcatcagg agcacactcc gacaacgatt aatcgcagtc
     9061 cagagaagca gaattgaagc aatgctccat aaaagattat tccattgcat ccactgtgga
     9121 taaatggcaa cggccagccc ccacaaccca aatattaacg cgcgatcgct tttgccaaaa
     9181 ggaccgacat aactacgaat accgttaatc gtctgcgcga gtaaaccgca aaactcagtc
     9241 aatatggtgc aaaataacat gagtattacg agtgatgcgt tactttccgg taaaaataaa
     9301 aacggtaaat agagcgcaat atcggaaata acatcgccag tttcatttaa aatcgccccc
     9361 agacgtgttt gctgattgca ctcacgcgcc aacatgccat ccagcgcatt gagtgccata
     9421 cggataaaaa gcacgatggg caatagcaaa aagaggatgg gttgtgcgac caacatcaac
     9481 agcaatccgg taataagaga aagcgccagt gcagcaagag tgatgtgatt cgctgtaaca
     9541 tggtgcttat aaagccaaaa catcgtcggc cttaacagcg actgaaagag cggttttatc
     9601 tggtagagcg tcattatcaa tccttgataa ttcggtttgc gctatggtgc atcaattccc
     9661 ctgacggaga tatcgcaaca cgacgcttca ttaaaaattc agaatatatc aaggtattgg
     9721 ggactcactg tcaggaatga ttatccccaa taaatcaagt ggttaaaaat tattacgctg
     9781 ttattcaata caactcatca agttttctca tcgcttgttc ccgtattgcg cgcgtcttac
     9841 cgttaagcgc cagctggcga actaaatcca gttcagggtt aacaagcttt aacgccagca
     9901 aaggatcacg tttcagcctc gcctttaata catcaactgg aaaacgggga ttattcatcg
     9961 ccacaatcca ccactcggga tcaatttctg ccgccagtac tgcaggcggt gtgtggcggt
    10021 tgttaagtgc gccgaacttg aaaaatgtga gattgctttt cgccagtgta ctccaccata
    10081 tacgatccgg ttgttttgcc agtacatcca gttgtgctaa cgcatcgggg tctagtgcat
    10141 aataaacacg taacgcttcg ttaacctgcg cacgggaact gttttcctgc ctcagaaaaa
    10201 acttcgccca aatatcattg cgtggcgtgt tttgctgtaa ttccttgaga acaacagcgg
    10261 gtaaattatc ccaactaatt aatgcactct ctaaaaagtc ccagtcgtcc gaacgtatca
    10321 gagaggtggc atttaatagc atctggtcat caacttcacc gtagctgata agctcagaca
    10381 aaatcatatc agcgctggtt tcatatgcat ctgaatctgc tttgggatta tttttccgat
    10441 gcgataaagc acgttgccaa attttttgtt tcatcgacgt gctcagatac tgatttttcg
    10501 ccagattact ccacccctca ggatcgttga aacgtgtcaa taaccgttgt aaagcagcgt
    10561 cagagtgaat tatctgcata tcccgacagc aaaagtaggc aggggatttt ttctccatcc
    10621 ttttaacaaa attaaactgt aagttatctg gcaacgctaa aaataacgca cctattagat
    10681 catcgtcatt ctcgaatttt tggtataaat atactgctat tttgtcactc tcttgtggcg
    10741 tcatccgcaa caattgacca tggtgtttca tctctgccag ctttaacgct aagcttgtga
    10801 caacatcgtt tgatgaatcg ttcgccaact gcattgcctg attgtaggtg agttgtcctc
    10861 cagcaccgac ctgacgaata gaggaatcat cactgcggat atagccatca ataatggatt
    10921 cgggcaataa ccacgcatct ttataaagct cctgaatgat catatcccaa tctcttttcg
    10981 ccagagtcat gacgatatcc agagggagat tttcacgtag cgcaagattc aacggaccga
    11041 caccatcagg tagatgactc attcgcgtca ttatttcgac agagagcggt tcacggtcga
    11101 gcaaccccag aacaacaggc tggtaaccca attgctgatc ggtcaactcc agaatctcat
    11161 tgtcaggcat atatctcgcc atactttcaa tcgtttgtcc atcattgcta ttaataagtt
    11221 gtttgcgtag gttagctgaa attcctttaa gccttgggag agttcgcatt atggcaagat
    11281 caaatgtcgc aatatctcgt ccttcaaatg cagcaacgtt gccttcatcc agcatttgca
    11341 attcctgcac taactcagga gaaggcttaa ggcgaaacag cgcttctttt ttcatctgat
    11401 tacagacatt ccctgccatg cgaatattgc ttcgcttaaa aatttctttc agagcatctt
    11461 catcatttat tagaccccaa taatatgcat caggatttat tatcatcagc gcttttttga
    11521 cattttcagt gtcatttttg tcactcgcac ggaccatgaa ttcactattt gtcgaagacg
    11581 caacaacagg tgcaattatg tcattgataa atatcccacg cgcattaata ctccccttaa
    11641 tcgtagacgg attatcgtaa tatgagtcga taaataccag tccacttgca tttatcgaac
    11701 cggtaattaa cagtggtgaa tccgtaacta tattcgttgc cgtcacgtta ccgagaataa
    11761 cccatggatt agaaagagag aaagtggaaa tattaccttt gactgtcaaa ttgccaagaa
    11821 caatcaattt ttgattactc tctatattac cttctaccac taaatcgcca cgatgcactt
    11881 tccagtccga tgcagataaa ccgaagggga caagattcac gctactgttg atagcttgtt
    11941 caacttgttg tttatcttca gcggtagccg cagaaaaaaa aggttcaata tcaactgcat
    12001 ttgcaacgca tcgaagttcc gcctgtactg gaagactcag cataaacagg ggaagtaaac
    12061 ctatccagct gcaaagcctg ggcttaaaca tatgcactcc atgccattaa aaaagaaaca
    12121 aaagggaaat gcatctttcc cttttgtata ttcgttattc tcgcgaaata ccatccaacg
    12181 tagacaacac tttctcagaa agatgcaact tttcagcagc catattctcc cgtaaatgcg
    12241 caacagaaga cgtccctggg atcagcaaaa tattcggtga acgttgtaac agccacgcca
    12301 gcgccacctg cattggtgtt gcacccaagc tcgcagcaac atcggaaagc gtggaagatt
    12361 gcagcggcgt aaagcccccg agcggaaaga acggcacgta ggcaatgccg tcacgagcca
    12421 aagcttcaat cattgcgtca tcagcacgat gagcaatgtt gtattcgttc tgcacacaga
    12481 cgatttcggc aatcttgcgc gcctctgcaa cctgcgtcgg tgtgacgttg cttaggccaa
    12541 tatgttttac caggccttgt tgttgcatct ctgccagcac ggtcaggctg gcttcaatcg
    12601 atccttccgc cgggccatga ccatccccca tcatcacgcg caggttaacc acatccagta
    12661 cgtccaggcc gagattacgt agattatcgt gcaccgcttt ttgcagctct gccggagaaa
    12721 atgcgggcaa ccaggatgcg tcctctccac gccgcgcacc aattttagtg acaattgtca
    12781 gatcgtcaga gtaaggataa agcgcttcgc ggataatctg attggtgacg tgcggaccat
    12841 aaaagtcgct ggtatcaatg tgattgacgc ccaatgccag cgcctcacgc agcacggtta
    12901 tagcgacgtg acgatctctt ggggggccaa aaaccccagg acctgccagt tgcatcgcgc
    12961 cataaccaag acggttaacg gattttgtac cgagagtaaa tgtattgctg ctcatcgcac
    13021 cctgccctgt aataaaacgg attaagaaaa atcagtatag aaacgaaaag agataaataa
    13081 tgcgaaggcg taaacaagat ttctggatga gtagcactat tgtcctgaaa gacaccaaaa
    13141 cattagacag caaaagataa gtcaagcgtc gcatccggca ataaaaaacc gcaacctgta
    13201 tcagcgttgc ggtttgtgta tcacatgcta aaaattagaa cgttttctta aagttcagca
    13261 tcacgccttt gtcgctgatg ccgtcctctt tccagtgata cgcatccaga ttcagcgaac
    13321 tttctttgct actgtacttc acgccgacgc tcgccaggct gttgataccg ccgcccttct
    13381 gaccgtcatc gacgttaaag tgctggctgc ctgcccctgc aaggcttgcc gtgcgctggc
    13441 tcttgctgta gctcaggttc ggcccgcctt ccagcgtcgc attcgcgctc cagccgcctt
    13501 tccctgcgta gtccagtttc agcccgacga tgctgtccac cgccgtttcg ctgccgctgt
    13561 tcatgctcag gttaaagtct ccggcattgc gctcctgata gccgccttcc agcgagtgac
    13621 gcagtttcac tccggcgtac ggggtgattt tcagcccttc gcgcggctca aaggttttcg
    13681 ccccttcgct acgcaactcc aggtactgct gtttcacatc cgtatccgcc gttttgctga
    13741 cgtcgccgta agcgaccgag cggctgctgt cgagattatg cacgtcataa cgcagcgcgt
    13801 tgttccaacg cataccattg tcgaaggaca tctggtgctt caggccaaag aactggctgt
    13861 agccgccggt tacgccattg tcgcccgctt tctgcgcacc gtcgccatcc agacgcgcga
    13921 taccgtattc caggctcatg ctctggcttt cgctcaggtc aacggtttta cgcagtgcca
    13981 gcatgtcata ctcggtgtta tttccgagtt ccgcacgcgg gtcacctttc gccaccacgt
    14041 taaaggccag gccattgccc accttcggcg cggcatccgc cagcatgctg aagcggttgc
    14101 ttaacacacg cgcttcacgg aataccgtgg tcgcctggct accgctcacc tgcttcaggg
    14161 cgctattcag ttcagcagtg gtgcccacgt tcaggctggt atacagctcg ttattggtgt
    14221 aacccgcatc cagtgccttc gccacatcgt tcaccgaagc atcggtcgcc acatcggtgt
    14281 aggcgttttt gctcatgatg acgtcaacgt tgccgctggc atcggtgctg cctttggcgg
    14341 tccataccac ggacgttgag gtgatggcat cggcgtcggt caggttgatg ccttccacca
    14401 cgttgtcgaa actgaccgtg gtgtctgccg taccggcggc gaaaccggtg ttgatcccga
    14461 caccattcat gctggcgttg ttgaccttca gtttaccggc gctgccgtca acgttggtac
    14521 cgacgatata gccgttgagg ttgttactgc tgccatcgct actaccgcta tcagtcgttt
    14581 cactgaaagg tgaagtggtg taagtgtttg gcatgtccgg caacgtgtag tcggtgtaat
    14641 gcacttcggt attgttaccg ctgttgccgt tcaccccttc cacattcacg ctgtcgccct
    14701 gcttaatcaa ccccgaacca gtcacgccgt cggcaatcac caccgtaccg ttgttaacga
    14761 tatgaccttc tgtgcccagt acgctgaacg caaacgagtc gttagcaaaa atattgatcg
    14821 tgccgttgtt ctcaattacc gcatcggcgg tggcattggc atccagttgc ataccagtca
    14881 atccggtatc ggtggtgcct tcggtaccga gattgataac gccgttgtta accagctgtg
    14941 cgtttttatc gccttccatc gccacggcgt tgttgccgct gatgttcatt acaccatcgt
    15001 tgagagcaac atagttgttg tgggtgaatt taaccgcgct ggcgttgtct ttatcgaagt
    15061 taattacccc gccatcctgg ttccacaaga aggcatcgct ttctgtcgca ccggcagtac
    15121 ttaccgccac ggtgactttc gcatccacca cgcccttgtt aataaactcg ctggcgccat
    15181 cgtcgtagtg aatagcgccc gcacctaatg cggcatccag cgtcatttca ccggtgttat
    15241 ataacgtggc gttagcattg ctgccctgga agcgaatggc gtttttgctg ttatcaagga
    15301 tgagtttgcc gctgttgttg aacgttgact tatcgtcaat atccagcgtc ccggcattgt
    15361 tgttttcatc ctgataaccc attttccagg tacccgcgtt attcactacc gcagagttat
    15421 agagaatcgc gcctttacgc gcctcgccgc ctgcaaggtt attgaatacc gcatcgccct
    15481 gagcgttaac gcgcgccgac gcaataccgc tgttattcac cgtaccatcg tgaagttgta
    15541 aagttgacag ggaaccggtg ttattaatta ccgcgttgct gtttttctca atttttacga
    15601 cgtcattaag cgttccgttg ttattgaagg ttcctccttc cagagtcacc gtgttcaata
    15661 caccttcatt ggttaacgca ccgccttcat tcacgacgat ggctgagcta atggttccgc
    15721 tcgactcgtt aaccagcgca ccattttcga gtatcctgat cgttccattt ttaacagcac
    15781 ccgcactggt gacatccccg ttgataatcg cacctgacga taataatacg gtctcacccg
    15841 cagaacttaa tacagcgcca tcatcatata aatcagcaat ataattatcc gttggattat
    15901 attcatcggc cccctagcaa acgccaaagg tacaaattgt gccatagttg ataataagcc
    15961 cagtgccatc gttatagaat gcctgaccat tttccgcgtt aatgttgatt acgcctgtct
    16021 ggtcattaat ggcggtgcct tcgtgataaa ccgccatgcc gacgagttta ttcacaccga
    16081 gagaatcgtt gtaattttca tttttctcca ggttgatggt tccctggtta atgaccgtat
    16141 tagtattccc atacgccgcc atccccgcac cggcgttgta gacagtaatc acaccattct
    16201 cgtggttggt tgccgtggct ttagttaatg ccccattata ggcatcggta cctgaagcca
    16261 ttcccacacc gaaatctttc gccgtgctat ttgcaacatt attacgcagc gatgtttcat
    16321 cattttcatc aacccacagc gtatccaggg tgatttgccc ttcgttatcc gcggttgatt
    16381 tatcgatggc tagcatccct acactgccac ggccatacat atcaataata cccgtttcgc
    16441 cgttcagaat ggatgacgag tagctggcaa gcatacctgt cagccctaat gccttgttat
    16501 tgaaagaggt accatcagac aagttccact ctgccggata attcagcaca ctgtgctgat
    16561 tcatcatttt ggtggtcatg gtaccattgt taacgccaat cccaccgtat tcaacatcaa
    16621 agacttttgg tgccgggtcg gtaccaaaat cgtacatcgc caggccaatg gatccgttat
    16681 tgattaccgt tgaatcttta ttccaggcag ccgcaaaacc gatatttttc agttccactt
    16741 caccgttgtt tgtcgccttc gcaccagaat caatactaat ggtggataac gctacacgat
    16801 aataataagt gaaagaatcc acatcactca tatcaaacgt tgcgtcagcg ccaatctcca
    16861 gctccgcgcc atggctcaac tgaatgagat tttcgaaccc cagcgggaac tcgccgctga
    16921 tggttgactc accattcagg tagaccgggg aatgcccatc aacgttaata agcggtgtgt
    16981 ctccgatacc acgtcgagaa gctgatggtt ttgagccatc atccatgaca tttttttcac
    17041 cgacaaaatg gacaccgcca ttaagcgtaa aggtattgtc atcgccagca ataacaatac
    17101 ctgcaatgtg ttcctgagca ccgtcgtaac tcgcatagga acgcgacgtg gttttactat
    17161 cagcaacaac ggttaactga ccatccagcg tgacgtcgtt gttgtcaccg ctgatattta
    17221 ccccggttga gggattaaag aagtaaggct gcgcattatc ggctttctga tctttatcaa
    17281 ccagaatact gcccgctatt tcgatagtat taccatcacc cgaaacatta acacccgtcg
    17341 ctttttgccc tgtcacattc agctcataag tcgccagcgt catgttgtta ttattgccag
    17401 agacatcaag accggtggag aaatccccaa cgtctaaccc acccgcgagt gaaatattag
    17461 ctttatcccc agcaacgctg acaccggttc cggcattggt cgcggaaata tcaccaacgt
    17521 taacaaatgt cgcattatca ccgtcgatct gaatgccaat ggcattttca cccgctgcgg
    17581 cctgaccaac attgaggtcg cccatgttga tcacgctgac gccatcacca tcaagcagca
    17641 tgccaataga gttatcttcg atgaccgtaa tattaccttt gttgctaacg aacgtatcat
    17701 tgccgtcaac gtcaataccg tgcgcaccac cgcttacgta taagtcgcct tcctgtttga
    17761 tggtggcacc gtcaccgaca attttcgagc cagtggaatt ctctccgtca acgctcatgg
    17821 taccttgatt atcgacccgc gcattatcgc cattgataag gctacccgta ccaccattgg
    17881 tgatggtcga gtcgcccgca gtgtttagct tggtattatc gccattgatg attaccgcgg
    17941 taccgccatc aacttccgtc tcgccattat tgttaatggt gatattgtcg ccagtgagta
    18001 taccgacaac cgagccttct ccgatggccg tcgttttgcc gtcgttgttg agggagatgt
    18061 tgtcaccatc aaccgtcatg acagtaccgc cattcagcgc cgtgttgccg ccctgatcgg
    18121 tgatgatcgc gttaccaaac tcaatcgcat tcccggttgt gccgtcaaca acgacgccgg
    18181 cctctttagt gatgtggatg agtccatcat cgtcgtaact ccaggtgatg ccacctgagg
    18241 tatttacgcc atcaaagaca acggtgttgc tgtcgtcatg gactgtccag gacttaacta
    18301 tcgtctcctt gccctcaggc gccgttaagg tgtagctccc atcggcattt tggctgtagg
    18361 taaaggtagt ttcgcgaatt ttgacagttt ttgcatcctg atcccaagtt acgccatttt
    18421 tatagacgac aggattgtgt ttaaccgggg tatcgccacc atcgtctggc ggaactggcg
    18481 tatcaccgcc gtcatctggc ggaaccggcg tatcaccgcc gtcatcaggc ggaactggcg
    18541 tatcaccgcc gtcgtctggc ggaacaggcg tatcaccgcc atcatccgga actggggagt
    18601 tattatggtg gtgattgtca tcgtcactta aggtatatat agcaattccc gtagctactg
    18661 cagcaagacc tgcagctgcc catggtaaaa ctgcactatc tttattttct ctgcactccg
    18721 cggataaatt ttctagctgc tctttgctca gggaatgaat atttgccgga catgttatct
    18781 tactggactt acgtgttttt tcggttgagt cgggtattga agtatccgca gcccatgtct
    18841 ggctacttaa cgccatggca acacaaacag acaataattt tttcctttgc atataaaatt
    18901 ccttagatct agaccgtgcg taacaagcca tgcagaattt gaagataaat cgtagtaaat
    18961 attcatagtg aaataagcag taaagaaatg cttatataat tcacaaaatc aatattaaaa
    19021 tcaggcaagt aaaagcatac aaaaaaaaca gttgatggaa ttatattcca caaaaaactc
    19081 acttcaatct aaaaattaac gacaaaaaac aaaaacacac taataattca ttgacgctgc
    19141 atatatattt tgtgattata tatatattgg taatgatttg ttatttaagg gaaggatata
    19201 taaggttttt gtagaaatac ccacggtaat taagcatgct aacgtcagtt taaatactgt
    19261 aaaagatcat tacattaata gatacatttt gtagccaact ataatttatt tgcgcaacca
    19321 tttaccatta actccctgaa catattgccc cgacttcgcg cgagcaacaa gcttttgtcc
    19381 agccagtttg gctatctctt tagtggatac attattttgc tttgccagtt gttggtaact
    19441 atggttacga gcttcattaa tatcttttac taatgcctgt gtttcggcat ccgtttgtag
    19501 cgccaccagg taaccgttaa gcgtttcacc tacccgcccc tgacttctgg cttcgttcag
    19561 ggttaaagcc attgcactgt cgctcatcag gccaatggcc catacagcta tgagtaatgt
    19621 tcttttcatc atcacctcaa aatagatcgc tacgggtttt gagcaggtct tcaacgtcct
    19681 tatccgcttt aatgatgatt tcatgctcaa ttttaacgtt catattaatg gtgattggct
    19741 ctttcggcgc agccacttca atgcgaggcg tgcaaccaac caacataaaa gaggacgcaa
    19801 acgcggctat cactattttc attgtttttc ctcacactct tttcctgatg ggcagccagt
    19861 ttccggtagc tgggcattct gctccagcca tacctggaga ttatcgccaa agcgtaaact
    19921 gcgccataac gtaaaaatat tctcttcatg gtgataatta aggtttaccg taccgctttt
    19981 accatcgacg cgactggtgc ccgaaatgtt ggcctgcatg gttaacacac cgagattatc
    20041 taaattaatc cttgttgatg agcgactgat ttccatataa cgcaaccagt tgatggcagt
    20101 tccagcggtc atattgtctt tggccaccgc atctgcagtg tctttatcca gacgcagcgt
    20161 catcggcccc gcgtttgcca accagccatc tttgacaatc catttttcat tgttcagcca
    20221 tagtggcaac gccccactaa atgccccaga catagcaaac tgtttcggat taaccgcgct
    20281 aaccagttcg ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat
    20341 acgtaattgt ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag
    20401 caatggttct tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc
    20461 tgtcaccaga ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg
    20521 ccaggctcca tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc
    20581 tggcatccag gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc
    20641 aggcgcagca gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca
    20701 gtcgggtggc accagcggct ggaatacagt cagtgattgt ttaggccacc aggcgttacc
    20761 gcgcagacga ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg
    20821 taaatcgcct ttaaagagga aatacgtcgg atcgcgccca tcgacgctaa attttaaggt
    20881 tgatggcggt aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc
    20941 gccgctgaag gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa
    21001 tcgcggcttt tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc
    21061 ggtcagcgta atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt
    21121 catcggtgta aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag
    21181 aaaatcattc gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc
    21241 ctgcaaaatg gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa
    21301 aggccagcgg atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa
    21361 cgcgcctggc tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa
    21421 acgggcatat aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagctg
    21481 gctgttatcc atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc
    21541 ttgcccctgg gtcagcacac tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg
    21601 ccagttgtcg actttgacac ccagtcggcc actcaaagga aaacctgcat acggccagct
    21661 ccagcgacca tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa
    21721 cggatcgccg ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag
    21781 ctcggcatcc accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact
    21841 tacaggaagt ccatccggca tgagcggcat agtaaattcc cccaccagtt taaccgacgg
    21901 ctgattttca aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg
    21961 gccttgaaat ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc
    22021 gagagagagt tttccctgcc attcctgcca gggagaaaaa atcagtttat caatattgat
    22081 ccaggtgtta ggcagcatgg actgccattg cgcgagggtt tttggagcag ctggcgattg
    22141 ctccgtctgc ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa
    22201 taaccagcgg ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc
    22261 caccagataa cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc
    22321 taatgcaata cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc
    22381 gagcgtcatc agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc
    22441 tttatattta cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa
    22501 tacgggtatt gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt
    22561 tgttgatgaa tttttcaata tcgccatagc tttcaattaa atttgaaatt ttgtaaaata
    22621 tttttagtaa cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt
    22681 tatagcacaa aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt
    22741 gagctggaat tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc
    22801 gaagcggtat gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa
    22861 aagcacggcg ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac
    22921 gcggcaaaag aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt
    22981 gctgaacacg ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag
    23041 cgtacccgtg acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa
    23101 acggcaggcg ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt
    23161 tttggtatgc gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt
    23221 gtggagtatg tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc
    23281 ccgctgacac cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat
    23341 ggcgtgatga tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa
    23401 gcgctgaaaa atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat
    23461 ctgttctttg aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct
    23521 gcctgccaca acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc
    23581 agtatttctc agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg
    23641 aacgaactgg tttaatcttg ccgctcccct gcaatccagg ggagctgatt cagataatcc
    23701 ccaatgacct ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa
    23761 ccacaatgaa gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa
    23821 gtaatgacaa aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa
    23881 gcgtaaacgg taagcccgtg acctgcgata aaaatccgcc agaaatcagc tttggtgaaa
    23941 aaatgatgat ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg
    24001 gtgaactgac agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta
    24061 atgaactcga taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg
    24121 cgaaccagtt aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa
    24181 tgaattaata gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt
    24241 tgggtaatgc acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac
    24301 cgccgatcat tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg
    24361 ctacgtgcgc cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag
    24421 cggcacaccc tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac
    24481 gacaccgcca taaataatag gcagcacaga gggcgacgtc gagagctgtc ctgcgcgttg
    24541 ccccgcattt ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat
    24601 tacgctgatg tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga
    24661 aagattacta ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg
    24721 tcatatgatt actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga
    24781 agttatcgcc atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg
    24841 ggccggaaac ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc
    24901 ggaagcgggt gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc
    24961 gtttacgtca tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga
    25021 actcacaccg tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactctctat
    25081 ttttggcgat cattccgacg tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc
    25141 ggcaaacgtc caggaagcgc aagactttgc tctcatttcg cacatcgcga cgctgaaaag
    25201 ccgcgtgcca tttattcatt tctttgatgg tttccgtacg tcccacgaaa tcaataaaat
    25261 tgtcccgctg gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca
    25321 tcgcgcccgg gcgctcaacc cggaacatcc cgtgatccgc ggtacgtcag ccaatcctga
    25381 cacttatttc cagtctcgcg aagccaccaa cccatggtac aacgcggtct atgaccatgt
    25441 tgaacaggcg atgaatgatt tctctgccgc gacaggtcgt cagtatcagc cgtttgaata
    25501 ttacgggcat ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg
    25561 tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg
    25621 cctgtaccgt cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag
    25681 cgtggcggta ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga
    25741 tgtaatgacc gcactggcag aagcctttaa taatggcgag cgcgaaactc tgccccgtgt
    25801 cattggtggg cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt
    25861 atttgccgag ctcaacgcgg ctaaaccgaa agcgcgcttt acggttggta tttacgatga
    25921 tgtgaccaat ctgtcactgc cgttgccgga aaacaccctg ccaaactcgg cgaaactgga
    25981 agccttgttt tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa
    26041 gattatcggt aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa
    26101 ggcgggcggc ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta
    26161 tctcatttcc caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca
    26221 gatggctgag cgtttaaaac ctggcggcat tttcctgctc aacacgccgt acagcacaga
    26281 tgaagtgtgg tcgcgcttgc cgcaggaagt tcaggccgtg ctaaatcaga aaaaagcgcg
    26341 cttctatgtg attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa
    26401 taccgtcatg cagatggctt ttttccatct gacacaaatt ctgcctggcg atagcgccct
    26461 cgcagaattg cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga
    26521 acgcaactgg caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc
    26581 ggtaaatccg cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgctttcgt
    26641 gaaaaccgta accgctgcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct
    26701 gccgccagac ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga
    26761 agagatcccc atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg
    26821 cccacactca gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc
    26881 cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca
    26941 ggtggctccg gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga
    27001 ccgtcagaat ccagagatta aagccatcaa tatgatgtcg cgcctggaac atgtcgaaga
    27061 agagaaaatc aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga
    27121 acgtattgat attcgcacat cgcagctgat tacaccgctg tttgaatatt ccggtgcttg
    27181 ctccggttgt ggcgagacgc cgtatattaa attgctgact cagctctatg gcgaccggat
    27241 gttgatcgct aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc
    27301 gtataccacc gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa
    27361 tgccgaattt ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg
    27421 tctgctggat caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga
    27481 cgccacgcca gaggttcgtc gtgaacaggt tgcagcttta cgccagcaac tcaacgatgt
    27541 tgccgaagca catgaactgc tgcgtgacgc agatgcactg gtggaaaaat caatctggct
    27601 gattggtggc gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag
    27661 tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg
    27721 tcaggcgtcg aaagcgacac cgctgggtgc agtaactaaa tttggcgagc acggcaaacg
    27781 taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggctca
    27841 gatttctctc ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta
    27901 tccgggacca tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc
    27961 actcagccat gaccagatgc gccaactcac agctaccggc ttctggccgc tatatcgctt
    28021 cgatccgcgt cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc
    28081 agaagcactg gaagaaacgt tacttcacga gcaacgtttc cgtcgtctga attcgcagca
    28141 gccagaagtg gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga
    28201 cttcctggca caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat
    28261 aaggattatc caattctaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg
    28321 ttggaaaatc agtggcaatg caatggccca acagaaaata aaatctgtga caataa
//
LOCUS       DAZFKY010000046        28358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFKY010000046 DAZFKY010000000
VERSION     DAZFKY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..472
                     /locus_tag="ACHKCL_001761"
     CDS             <1..472
                     /locus_tag="ACHKCL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762744.1"
                     /translation="GTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVA
                     NIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAY
                     QEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            595..1221
                     /locus_tag="ACHKCL_001762"
     CDS             595..1221
                     /locus_tag="ACHKCL_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762745.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1260..1505)
                     /gene="zapB"
                     /locus_tag="ACHKCL_001763"
     CDS             complement(1260..1505)
                     /gene="zapB"
                     /locus_tag="ACHKCL_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5762746.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            1931..2776
                     /gene="glpF"
                     /locus_tag="ACHKCL_001764"
     CDS             1931..2776
                     /gene="glpF"
                     /locus_tag="ACHKCL_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5762747.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2799..4307
                     /gene="glpK"
                     /locus_tag="ACHKCL_001765"
     CDS             2799..4307
                     /gene="glpK"
                     /locus_tag="ACHKCL_001765"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5762748.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4537..5547
                     /gene="glpX"
                     /locus_tag="ACHKCL_001766"
     CDS             4537..5547
                     /gene="glpX"
                     /locus_tag="ACHKCL_001766"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5762749.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5644..6390
                     /gene="fpr"
                     /locus_tag="ACHKCL_001767"
     CDS             5644..6390
                     /gene="fpr"
                     /locus_tag="ACHKCL_001767"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5762750.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6395..6823)
                     /gene="uspD"
                     /locus_tag="ACHKCL_001768"
     CDS             complement(6395..6823)
                     /gene="uspD"
                     /locus_tag="ACHKCL_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5762751.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6850..7149)
                     /gene="yiiS"
                     /locus_tag="ACHKCL_001769"
     CDS             complement(6850..7149)
                     /gene="yiiS"
                     /locus_tag="ACHKCL_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5762752.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7361..7801)
                     /gene="yiiR"
                     /locus_tag="ACHKCL_001770"
     CDS             complement(7361..7801)
                     /gene="yiiR"
                     /locus_tag="ACHKCL_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5762753.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            7902..8501
                     /gene="yiiQ"
                     /locus_tag="ACHKCL_001771"
     CDS             7902..8501
                     /gene="yiiQ"
                     /locus_tag="ACHKCL_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5762754.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8609..9376
                     /gene="tpiA"
                     /locus_tag="ACHKCL_001772"
     CDS             8609..9376
                     /gene="tpiA"
                     /locus_tag="ACHKCL_001772"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5762755.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9431..10186)
                     /gene="cdh"
                     /locus_tag="ACHKCL_001773"
     CDS             complement(9431..10186)
                     /gene="cdh"
                     /locus_tag="ACHKCL_001773"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5762756.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10293..11282)
                     /gene="sbp"
                     /locus_tag="ACHKCL_001774"
     CDS             complement(10293..11282)
                     /gene="sbp"
                     /locus_tag="ACHKCL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5762757.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11601..12563)
                     /gene="pfkA"
                     /locus_tag="ACHKCL_001775"
     CDS             complement(11601..12563)
                     /gene="pfkA"
                     /locus_tag="ACHKCL_001775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5762758.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12744..13646)
                     /gene="fieF"
                     /locus_tag="ACHKCL_001776"
     CDS             complement(12744..13646)
                     /gene="fieF"
                     /locus_tag="ACHKCL_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5762759.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13795..14295)
                     /gene="cpxP"
                     /locus_tag="ACHKCL_001777"
     CDS             complement(13795..14295)
                     /gene="cpxP"
                     /locus_tag="ACHKCL_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5762760.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14445..15143
                     /gene="cpxR"
                     /locus_tag="ACHKCL_001778"
     CDS             14445..15143
                     /gene="cpxR"
                     /locus_tag="ACHKCL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5762761.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15140..16513
                     /gene="cpxA"
                     /locus_tag="ACHKCL_001779"
     CDS             15140..16513
                     /gene="cpxA"
                     /locus_tag="ACHKCL_001779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5762762.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16619..17293)
                     /gene="yiiM"
                     /locus_tag="ACHKCL_001780"
     CDS             complement(16619..17293)
                     /gene="yiiM"
                     /locus_tag="ACHKCL_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5762763.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17442..18425)
                     /gene="kdgT"
                     /locus_tag="ACHKCL_001781"
     CDS             complement(17442..18425)
                     /gene="kdgT"
                     /locus_tag="ACHKCL_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5762764.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18685..19305)
                     /gene="sodA"
                     /locus_tag="ACHKCL_001782"
     CDS             complement(18685..19305)
                     /gene="sodA"
                     /locus_tag="ACHKCL_001782"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5762765.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19590..20624
                     /gene="rhaT"
                     /locus_tag="ACHKCL_001783"
                     /pseudo
     CDS             19590..20624
                     /gene="rhaT"
                     /locus_tag="ACHKCL_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20621..21469)
                     /gene="rhaR"
                     /locus_tag="ACHKCL_001784"
     CDS             complement(20621..21469)
                     /gene="rhaR"
                     /locus_tag="ACHKCL_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5762766.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21543..22379)
                     /gene="rhaS"
                     /locus_tag="ACHKCL_001785"
     CDS             complement(21543..22379)
                     /gene="rhaS"
                     /locus_tag="ACHKCL_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5762767.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22667..24136
                     /gene="rhaB"
                     /locus_tag="ACHKCL_001786"
     CDS             22667..24136
                     /gene="rhaB"
                     /locus_tag="ACHKCL_001786"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5762768.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            24133..25392
                     /gene="rhaA"
                     /locus_tag="ACHKCL_001787"
     CDS             24133..25392
                     /gene="rhaA"
                     /locus_tag="ACHKCL_001787"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5762769.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25567..26391
                     /gene="rhaD"
                     /locus_tag="ACHKCL_001788"
     CDS             25567..26391
                     /gene="rhaD"
                     /locus_tag="ACHKCL_001788"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5762770.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26401..26715
                     /gene="rhaM"
                     /locus_tag="ACHKCL_001789"
     CDS             26401..26715
                     /gene="rhaM"
                     /locus_tag="ACHKCL_001789"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5762771.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26756..28150)
                     /locus_tag="ACHKCL_001790"
     CDS             complement(26756..28150)
                     /locus_tag="ACHKCL_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5762772.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCCIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         7054 a         7262 c         7095 g         6947 t
ORIGIN      
        1 cggcacttgt tggttaatgg gattatttct tatctatagc agctatacgg tgtgggaacg
       61 acacagcata tgggattata ctttcccgga gccaggtatc accgtcacgg ttccgagcaa
      121 acagtgggtt gccaatatag taaaacaagg accaaccctg gtgacccggg atgcccatgc
      181 tattttagct attgttgcct tctcacaaaa tgaactcggt gtgcattcaa tcgaggaact
      241 taccgcgact caaaacggaa ctgcagaaat gcatgtttgt gatgttcagg gatttgcctg
      301 cgcttatcag gaaggagtgc aaacaatgag tgatggcaaa gtaagacatg ctattttcat
      361 gactctactg gataacacta atttgataca actggtcgca gctatcgacc cagattattt
      421 ggacgaatat caagaagaag taatgaaaat gatgctttct gctcgtcaat gatctgatgt
      481 gcatgttaat aataatggat tgatatgaga tacgttatct tattaatact atggataata
      541 actgtttttc ttagtcatct tcagcacagt aacttgctta ctggagatcc agggatggtt
      601 ggcgagtata tcggttcagt acttcttgga ccgttattac ttcccgtgct tgtgtcaggt
      661 attctgtgca cattcactaa aaaaacgcgt aacttcgcca gttttactcg cgggtgctgt
      721 tgggtattgg gagtcctgct tttatccaac gtcggcaata cgtttcgtct gttcaccccg
      781 tggcactata cgtttgagaa ggcagctatt tccgtgactg tcccaaaccg acactggaat
      841 acggtttcta ttagtactga taaaaccatt gatataagaa gtgaagataa tagcgtcttt
      901 atttctgctt tccgcctgcc cgcaggacgt agcgccgatg actcgctgga agagttaaag
      961 aagatgcagc gagacaatct caaggatcaa tacaatgaag aaaccttcca gtttcatgat
     1021 tgtaatgcca aacatttcac ctgtaagtat caggatgtgt tgataaattt tgatggtcag
     1081 cagaaaagaa ccatcagtgt ttatcttgaa gacaccccca gggctgtggg gatcattgca
     1141 ttaatggagc cagatacggc agataaatat cgccaacagg cgatggagat tatgttgtct
     1201 gctaaaaata cagtgaagta aaaaataagg cgccttgcgg cgcctctgcg tggaagagat
     1261 taaacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt
     1321 tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact
     1381 tcctgcgaca atgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg
     1441 atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt
     1501 gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg
     1561 gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca
     1621 tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg
     1681 gtgacataat ccacaccaat cgaaaatgtt aataaatttg ttgcgcgaat gttctaacaa
     1741 acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgaa
     1801 gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca
     1861 tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca
     1921 ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg
     1981 taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc
     2041 gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta
     2101 cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct
     2161 gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc
     2221 tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca
     2281 gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggcaggca ctttctctac
     2341 ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc
     2401 tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc
     2461 tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt
     2521 gacgggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc
     2581 gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct
     2641 ttttggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg
     2701 ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc cttcagaaca
     2761 aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg
     2821 ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata
     2881 tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc tgggtagaac
     2941 acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag
     3001 ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta
     3061 ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc
     3121 gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca
     3181 ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc
     3241 atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata
     3301 cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct
     3361 ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc
     3421 tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga
     3481 ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc
     3541 aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca
     3601 ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctac
     3661 tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt
     3721 ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct
     3781 acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg
     3841 catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc
     3901 tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc
     3961 agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc
     4021 gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtct gatattctcg
     4081 gcacccgcgt tgagcgcccg gaagttcgcg aagtcaccgc attgggtgcg gcctatctcg
     4141 caggcctggc ggttggcttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg
     4201 agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga
     4261 aaaaagcggt caaacgcgcg atggcgtggg aagaacacga cgagtaatgt caggcacctg
     4321 aatcgattgc ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc aggcctacga
     4381 gccaatgcca ttttgtgtag gcgggataag gcgttcacgc cgcatccggc atcacgcaga
     4441 ctctgaaacc gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct
     4501 acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa
     4561 ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat
     4621 aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac
     4681 attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt
     4741 ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa
     4801 ggcacgcgca tgacggcgat gggtcaggct aactcgctgg cggtgctggc agtaggcgat
     4861 aaaggctgtt tcctcaacgc gccagatatg tatatggaga agctgatcgt cggaccggga
     4921 gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtcgcggcg
     4981 gcgctcggca aaccgttgag cgaactgacg gtaacgattc tggctaaacc acgccacgat
     5041 gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac
     5101 gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggaatt
     5161 ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg
     5221 aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt
     5281 ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aatccggtaa agtattgcgt
     5341 ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc
     5401 gatctgctgg aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc
     5461 cgcggcaaat cacgcaccat tcgccgcatt cagtctatcc actatctgga tcgcaaagac
     5521 ccggaaatgc aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg
     5581 ggactggaat ttttttgttc ggagaacgaa gataaggcaa ttcaatcaaa acaggagaaa
     5641 aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg
     5701 tttagtctca ccgttcacgc ccctgtgcat ccgtttaccg ccgggcaatt taccaagctt
     5761 ggccttgaaa tcgacggcga acgcgtccaa cgcgcctact cctatgttaa ctcgcccgat
     5821 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg
     5881 gcggcactga aaccgggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt
     5941 ctggatgaag taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc
     6001 ccttatttat cgattctgca actaggtaaa gatttagatc gcttcaaaaa tctggtcctg
     6061 gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa
     6121 aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg
     6181 tcgctcaccg gacggatacc ggcattaatt gaaagtggtg aactggaaag cgcgattggc
     6241 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc
     6301 gatacgcaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg
     6361 ggtcacatga cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga
     6421 gcaaatccgc actcaactta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa
     6481 atgagtggtg atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca
     6541 gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt
     6601 tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg
     6661 ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg
     6721 tcaggtgagc gtcattatgt ctggcgagct ccaaggcttt attcaccagt aaggcatctt
     6781 cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa
     6841 gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca
     6901 tgagaaggtg aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc
     6961 cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat
     7021 aaaggactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc
     7081 gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac
     7141 atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg
     7201 cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt
     7261 gcgtaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa
     7321 caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt
     7381 cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa
     7441 caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacgcccta
     7501 ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagtagc atccacgcca
     7561 caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta
     7621 ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta
     7681 aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa
     7741 tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca
     7801 tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat
     7861 atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc
     7921 tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag
     7981 atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca
     8041 gccagtttcg agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg
     8101 ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgccagtaaa attaacgaga
     8161 acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga
     8221 catggctacc catccagggg ccagagcaaa aagccgcgaa agcgaaagct caggaataca
     8281 tggcagcggt gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac
     8341 tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac
     8401 tgcgctatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta
     8461 agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt
     8521 gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt
     8581 tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa
     8641 actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc
     8701 aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa
     8761 gcgtgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc
     8821 cggcgcattc accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat
     8881 catcatcggt cactctgaac gtcgtactta ccacaaagaa tctgacgaac tgatcgcgaa
     8941 aaaattcgcg gtgctgaaag agcagggcct gactccggtt ctgtgcatcg gtgaaaccga
     9001 agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact
     9061 gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc
     9121 aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcattcg
     9181 tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg
     9241 ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct
     9301 ggttggcggt gcgtctctga aagctgacgc tttcgcagta atcgttaaag ctgcagaagc
     9361 ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta
     9421 actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac
     9481 ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc
     9541 gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca
     9601 gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt
     9661 catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt
     9721 tatccagctg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt
     9781 gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc
     9841 cgtatttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg
     9901 aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat
     9961 attgcagtgg gccattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg
    10021 cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct
    10081 tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg gcagccacaa
    10141 cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt
    10201 acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga
    10261 aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta
    10321 ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg
    10381 gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgccacctc agcgtcgcgc
    10441 ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg
    10501 taggcttccg ccacctcttt agtacctttt ttctcgacca ctttatcgac caccgacacg
    10561 gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt
    10621 tcattcgctg ccagcagagc ttcgttttcc caggaaatca gtacatcgcc aattccgcgc
    10681 tcgacaaaag tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac
    10741 agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag
    10801 ccccaggctg ccaggtagtt ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc
    10861 accgaaacac ccagtttaat cagatcgttc cagtcatgga tctgcttcgg atttccctta
    10921 cgtaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc
    10981 cactctttat caatccgccc gcgttccgca atcgcgtcca cgtcataggc cagagccagc
    11041 gtgacaacat cagcttcaat accgttgatt accgacgttg cttgtttgcc tgaaccaccg
    11101 tgtgactgac gaatcaccac gttatcgcca gtttgctgtt tccagtgggc gctgaatgcc
    11161 ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata
    11221 tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc
    11281 atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa
    11341 cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgggg gttaaaaagg
    11401 cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt atcgatgccg gatgcggcgc
    11461 gaacgcctta tccggcctac aaaagtttgt aaattcaata aattgtagaa ttcacgtagg
    11521 cctgataagc gaagcgcatc aggcattttt gcttctgtca tcggtttcag gctaaaggaa
    11581 tctgcctttt tccgaaatca ttagtacagt tttttcgcgc agtccagcca gtcgcctttg
    11641 aacggacgct tcatgttttc gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc
    11701 tgtataccta cgcaacgacc gccgtaacct gccagcagca gatcgatagc gtaagcgccc
    11761 atacgggaag ccagaatacg gtcgtaaggc accggagaac caccgcgctg gatgtggccc
    11821 agcacagttg cgcgggtttc acgaccggtt tctttctcga tgaaatgcgc cagttcgtca
    11881 acatcacaca tatgttcggt aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc
    11941 gctttgattt cgtttaccag gtcttcacgg ctgaattcaa cttccggaac cacaacgaat
    12001 tcacagccac cggcaatggc cgcagccagc gtcagatcgc cacaataacg gcccatcact
    12061 tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct
    12121 acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg
    12181 tcgatagtgc ccggcaggcc gatgcacggg aagcccattt cggtcagacg cattgcaccc
    12241 atgtaggaac cgtcaccgcc gataaccacc agcgcatcga tcccacgttt tttcaggttt
    12301 tcgatagcca cggcgcggat gttctcgtcg cggaattccg ggaaacgcgc agaaccgagg
    12361 aacgtaccgc cacggttgat catgtcagaa acgctgtaac ggtctagctg taccatacgg
    12421 tcttcataca gacccagata gccgtcataa atgcccatta cttccagacc ttctgtcagc
    12481 gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc
    12541 aacacaccga ttttcttaat catgactacc tctgaacttt ggaatgcaaa ataaaatctg
    12601 ttgccggaag tcttcttgca catcgaagtg atccaacgaa tgtgcaaata gtataacaat
    12661 cacttcctgc tgaattgatt caggtcaggc caaatggcgg tattttatac acaaaatgcg
    12721 ggtctggctc tcttttatac tgattatgaa agcatagacc gtttaccctc cctaggtacg
    12781 acggaacagg ggtcctgatg gataatcaca tccgatcccg gaaaacgccg taaaatagcc
    12841 tgctctacct gatccgccac catatgtgcc tgaaccaaag gcagagagtc ttccatttcc
    12901 aaatgaatct gaataaagcg ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg
    12961 ctaacacccg gccaggaagt cacgatatca ataatttctt gccgttcctc atcaggtaat
    13021 gcgcgatcca gtaatgactg taccgcctca tatcccatgc gtaacgcgct atataaaata
    13081 tagatgccga ttcccaatgc aaacagagca tcggcgcgat gccagccgta ccaggacaac
    13141 cccagcgcca gcagaattgc gccgttcatc ataacatcag actggtaatg tagcatatca
    13201 gcccgcaccg cctggctttg cgtccggcgc accacccaac gctgaaacga gacaaggata
    13261 atcgtacaaa ttagcgccac aattgtcacg ataaccccga cgcctggatc tgtcatcggt
    13321 gttggagata tcagatgttg aatgcctgtc aaaaacagga atagtgccga accggagata
    13381 aacatacttt gcgccagcgc cgcgagggac tcagctttac cgtgaccaaa cgagtgatta
    13441 tcgtcggcag gttgcaggga atatcgcacc accaataaat tcgtcaacga cgcgccgata
    13501 tccaccagcg aatccaccag cgcggcgaga atactcaccg acccggtata ccaccatgca
    13561 aaaattttaa tcagcaatag cagtgaagcc atcgccgtcg cagcaatcgc cgcccgactg
    13621 accagccgtc cataagattg attcataaat actcccgcta tcaactgacg ctagtataac
    13681 ggaagcaaat catctgcaat gcattaagca gcaggcaaat tgaggataaa aaaaaccccc
    13741 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    13801 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    13861 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    13921 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    13981 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    14041 gacaagacga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    14101 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    14161 catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga
    14221 accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc
    14281 ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt
    14341 ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta
    14401 gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt
    14461 tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct
    14521 tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg
    14581 atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac
    14641 ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc
    14701 gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg
    14761 agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag caacagcaaa
    14821 acaacgacaa cggttcaccg acactggaag ttgatgcctt agtactgaat ccaggccgtc
    14881 aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt accctgctct
    14941 atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc caggaagtgc
    15001 tgggcaaacg cctgacgcct ttcgaccgcg ctatcgatat gcacatttcc aacctgcgtc
    15061 gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct
    15121 acctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg
    15181 gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca
    15241 gatgaccgag cttctggata gcgaacagcg tcagggtctg atgattgagc agcatgttga
    15301 agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc
    15361 gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg aaggccgcgt
    15421 gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa
    15481 cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt
    15541 gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc
    15601 cgatttcatt aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgttggt
    15661 cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa
    15721 aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg
    15781 gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg cgctggagcg
    15841 catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc gcaccccgct
    15901 gacgcgtctg caactgggta cggcgttact gcgccgtcgt agtggtgaaa gcaaggaact
    15961 ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg attaacgacc tgttggtgat
    16021 gtcacgtaat cagcaaaaaa acgcgctggt tagcgagacc atcaaagcca atcagttgtg
    16081 gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt cgttgacagt
    16141 taacttcccg cctgggccgt ggccgctgta cggcaacccg aacgccctgg agagtgcgct
    16201 ggaaaacatt gttcgtaatg ccctgcgtta ttcccatacg aagattgaag tgggctttgc
    16261 ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga
    16321 agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcgcgcg atcgtgaatc
    16381 tggcggtaca ggtttgggac tggcgattgt tgaaaccgcc attcagcagc atcgtggctg
    16441 ggtgaaagca gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta
    16501 taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg catccggcat
    16561 ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac tccgggggtt
    16621 attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca
    16681 tcgttcgcgt ccagcttttc gataacccgg cagcggagag taaacggtga tactgatcgt
    16741 catcaaacgg catatgccat gcgatggcag cagcctcctg cacggtcaca tcgctgacac
    16801 gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc acgctataca
    16861 accatcccac tttacccgtg ttttgcatca actgcgctat atcgctgata tcaaaatggt
    16921 aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc tctccccagc
    16981 ggaaaatatc gccaatataa acattacttt ccgtgagacc gtcggttgag agattttcgc
    17041 caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc cagtagagat
    17101 aatgctcacg aggataatga cacagcgctc tgtctggtcc gccgtgaacc tttttctccg
    17161 cctgctcgtc accttccagc cccagctccg ttagcatcaa ctcaccatca acctggattt
    17221 tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg tatacatcaa
    17281 ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca tatttatttt
    17341 atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag caaaccggac
    17401 gaacgttagt tcatccggct gtggggatta aggaagcagg tttatttcac cgtacctaaa
    17461 atttcgattt tcgccgctct ggctttgact ttacgtgacc agatagaggt aagaattggc
    17521 accagaatcg aagtcacaat gactgccgtc gctaccagcg aagttgctgc cggagccatc
    17581 ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg
    17641 gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt atcggcgata
    17701 atcaacggaa taccggtcac gataattact gccacaccca acaggatccc cagcaaacct
    17761 gtctgggcaa ttacagtcaa atcaatggta ttgcctagtg cgaaggcaaa gaatggaatc
    17821 agcgtctgca ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt cccaagggca
    17881 aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc
    17941 ccggcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga catcaacaca
    18001 aatgccccag cttcttcttt tgtgccgtac tgctgcatga tggaagcgta aagtccgccg
    18061 ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa gaatccaact
    18121 tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca cgcgacggca
    18181 attttagtta ccaccagcgt accggattta cgcagtaccg ttcccgtcgc gcttaatttt
    18241 attgacgccc ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc
    18301 ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg gcacagtgcg
    18361 ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcaa gcgttttatc
    18421 tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtaattcc tcaatggagc
    18481 atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg atcaaaacca
    18541 catcaactga aacgctgttt tatttttatc ggatcattgt ttttattttg aaattgatca
    18601 caaaaaaacc accgccattg gcgatggttc attttttaag ctgatatgcg gcgtaaacgc
    18661 ctcactgcag cagacggcaa atgattattt tttcgccgcg aaacgtgccg ctgcttcgtc
    18721 ccagttcacc acgttccaga actctttgat gtagtccggg cggcggttct ggaatttcag
    18781 gtagtaagca tgctcccaca catccaggcc cagaatcggg aagccggatg cgccagaaat
    18841 agcttcaccc atcagcgggg agtcttggtt agcagtagaa accaccgcca gtttatcgcc
    18901 tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt tctcaaattc
    18961 tgctttgaag ttatctacgg aaccgaaatc acgttcgata gccgctttca ggtcaccctg
    19021 cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc
    19081 gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcaactc
    19141 ttcaaccggc aggttggcaa attctggcag gctttccaac gccgcgttgg cgttgttgac
    19201 gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc
    19261 cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat
    19321 tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg
    19381 atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg
    19441 cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc
    19501 ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg
    19561 acgcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt
    19621 ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa
    19681 aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt
    19741 aggccatcag cgccctgtta ctaccgaatt tctgggcgta ttacagctcg tttagtctct
    19801 ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactacg
    19861 gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt
    19921 tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ttgattaaca
    19981 ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg
    20041 taactcgagc cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc
    20101 tgaaaaaagg actggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg
    20161 cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc actaggcgtc gatccactgt
    20221 atgtcgctct gccaagctat gttgtcatca tgggcggcgg cgcgatcatc aacctcggtt
    20281 tctgttttat tcgtctggca aaagtgaagg atttatcgct aaaagccgac ttctcgctgg
    20341 caaaaccgct gatcattcac aatgtgttac tctcagcact gggcgggttg atgtggtatc
    20401 tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcagtat gactacatca
    20461 gttggatgct gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga
    20521 aagagtggaa caatgcagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga
    20581 ttattgttgc cgccaacatc gttggcatgg gcatggcgaa ttaatctttc tgcgaattga
    20641 gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt
    20701 tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga
    20761 gaagatattg cgcatgacac actctgacct gccgcagata ttgattgatg gtcattccag
    20821 tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt
    20881 tatccagcgc aaagggactt ttcaggctag ccgccaaccg ggtaatcagc ttatccagca
    20941 acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca
    21001 tcaccaactg cccgaacagc aactctgcca tttcgttagc aaacggcaca tgctgactac
    21061 tttcatgctc aagctgaccg ataacctgcc gcgcctgtgc catccccatg ctacctaagc
    21121 gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat cgccccctgc cagtcaagat
    21181 tcagcttcag ccgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag
    21241 cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc gccacgggta atgcgataag
    21301 ggcgatcgtt gagtacatgc agaccattac cgcgccagac aatcaccagc tcacaaaaat
    21361 catgtgtatg ttcagcaaag acatcttgcg gataacggtc agccacagcg actgcctgct
    21421 ggtcgctggc aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct
    21481 cggccagaga acgaaattga ttattcgcaa tatggcgtac aaatacgttg agaagattcg
    21541 cgttattgca gaaagccatc ccgtccctga cgaatatcac gcggtgacca gttaaactct
    21601 cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cgcaatgata ggcgatgtca
    21661 gtaacgctgg cctcgctgtg acgtagcaga tgtcgggctt tcatcagtcg caggcgattc
    21721 aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt
    21781 gaaagagaaa attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc
    21841 agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg
    21901 cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt
    21961 aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat
    22021 acgctgtggt taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac
    22081 tgattcagtc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attagtcaga
    22141 cacagattat cggtatgttc atacagatgc cgatcatggt cgcgcacaaa acagaccgtg
    22201 ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca
    22261 atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt
    22321 tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata
    22381 ctggcctcct gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt
    22441 aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt
    22501 caggaaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt
    22561 catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc
    22621 aggcgctttt tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa
    22681 ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg
    22741 tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt aacaatgggc tgcatagtca
    22801 gaacggctat gtcacctggg atgtggatag ccttgaaagt gccattcgcc ttggattaaa
    22861 caaggtgtgc gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga
    22921 ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag
    22981 ccgcaccaat ggcctaatgg cgcaggcaca acaacaactc ggcaaacgcg atatttatca
    23041 acgtagcggc atccagtttc tgcccttcaa tacgctttat cagttgcgtg cgctgacgga
    23101 gcaacaacct gaacttattc cacacattgc tcacgctctg ctgatgccgg attacttcag
    23161 ttatcgcctg accggcaaga tgaactggga atataccaac gccacgacca cgcaactggt
    23221 caatatcaat agcgacgact gggacgagtc gctactggcg tggagcgggg ccaacaaagc
    23281 ctggtttggt cgcccgacgc atccgggtaa tgtcataggt cactggattt gcccgcaggg
    23341 taatgagatt ccagtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc
    23401 gccgttaaac ggttcacgtg ctgcttatct ctcttctggc acctggtcat tgatgggctt
    23461 cgaaagccag acgccattta ccaatgacac ggcactggca gccaacatca ccaatgaagg
    23521 cggggcggaa ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg
    23581 agtgcttcag gagcggcaaa tcaacgatct tccggcgctt atctccgcga cacaggcact
    23641 tccggcttgc cgcttcatta tcaatcccaa tgacgatcgc tttattaatc ctgagacgat
    23701 gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc
    23761 tgaactggcg cgctgcattt tcgacagtct ggcgctgctg tatgccgatg tgttgcatga
    23821 gctggcgcag ctgcgcggtg aagatttctc gcaactgcat attgtcggcg gaggctgcca
    23881 gaacacgctg ctcaaccagc tatgcgccga tgcctgcggt attcgggtga tcgccgggcc
    23941 tgttgaagcc tcgacgctcg gcaatatcgg catccagtta atgacgctgg atgaactcaa
    24001 caatgtggat gatttccgtc aggtcgtcag caccaccgcg aatctgacca cctttacccc
    24061 taatcctgac agtgaaattg cccactatgt ggcgcggatt cactctacac gacagacaaa
    24121 ggagctttgc gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc
    24181 gcggcagtag ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca
    24241 atgcactgct ggcagggtga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc
    24301 ggagggattc aggctactgg caattatccg ggcaaagcgc gtaatgccag tgagctacgt
    24361 gccgatctgg aacaggctat gcggctgatt ccggggccaa aacggcttaa tttacatgcc
    24421 atctatctgg aatcagacac gccagtctcg cgcgaccaga tcaaacctga gcacttcaaa
    24481 aactgggttg aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgtttc
    24541 tcgcatccgc taagcgccga tggctttacg ctttcccatg ccgacgacag cattcgccag
    24601 ttctggattg atcactgcaa ggccagccgc cgcgtttcgg cctattttgg tgagcaactc
    24661 ggcacaccgt cggtgatgaa catctggatc ccggatggca tgaaagatat caccgttgac
    24721 cgtctcgctc cgcgccagcg tctgctggca gctctggatg aggtgatcag cgagaagctg
    24781 gatccggcgc accatatcga cgccgttgag agcaaattgt ttggcattgg cgcagagagc
    24841 tacacggttg gctccaatga gttttacatg gggtatgcca ccagccgcca gactgcgctg
    24901 tgcctggacg ccgggcattt ccacccgact gaagtgattt ccgacaagat ttccgccgcc
    24961 atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat
    25021 cacgtagtgc tgctggatga tgaaacccag gcgattgcca gtgagattgt tcgtcacgat
    25081 ctgtttgacc gggtgcatat cggcctcgac ttctttgatg cctctatcaa ccgtattgct
    25141 gcgtggatca ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct
    25201 accgctgagc tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct ggcactgctg
    25261 gaagagcaga aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca acgtcacgat
    25321 acgccagcag gtagcgaatg gctggagagc gtgcgggcat atgagaaaga aactttgagt
    25381 cgccgcgggt aaacactgcc ggatgcggca caagtgcctt atccggccta caggccggca
    25441 atagttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg ccggatgcgg
    25501 cacgagcgcc ttatccggcc tacgggtcgt gcatctgaca acaccgaatt tacaggaaca
    25561 cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
    25621 gatgcctggc tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat
    25681 gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc
    25741 agccaaccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc
    25801 ttccgtaacg tccagctcga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat
    25861 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt
    25921 ccggctcact ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
    25981 atcatgcact gccacgccac caacctgatc gccctcacct atgtacttga aaacgacacc
    26041 gcggtcttca ctcgccaact gtgggaaggc agcaccgagt gtctggtggt attcccggat
    26101 ggcgttggca ttctgccgtg gatggtaccc ggcacggacg aaatcggcca ggcaactgcc
    26161 caagagatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
    26221 ccgacgctgg atgaaacctt cggtttaatc gacaccgcag aaaaatcagc ggaagtgtta
    26281 gtgaaggtgt attcgatggg cggcatgaaa cagactatca gccgtgaaga gttgatagcg
    26341 ctcggccagc gtttcggcgt tacaccactc gccagtgcgc tggcgctgta aggagcaaac
    26401 atgatccgca aagcctttgt catgcaggta aaccccgacg cccacgaaga gtatcagcgt
    26461 cggcataatc ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac
    26521 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct
    26581 gaagaacggt ggaatgcggt agccagcact gaaatctgcc aacgttggtg gaaatatatg
    26641 accgatgtta tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg
    26701 ttttacctgc cttaatgagt tgcggtgccg gaaaatatcc agcaccgcga cttcatcaga
    26761 agaagtattt aaagcgaacg cgtgcgccgt aacggtcatc agtatcatca ccgtgttttt
    26821 tatcgccgtc gctatccaga atggagtaat acgtcccgag gtaaatatta aagttctcca
    26881 tatccatcac gttagcgaac tgataagacg catgaatggt gtgaatggtg tacgtccctt
    26941 catcatcaat gcagcagtcg ttatcacaaa ctacaccgct gaattcatca atcttattgt
    27001 gcgcgtagat ataaccgagc tcgaaacgtt tccacagcgc gttaatcccg gcagtgaaat
    27061 ctttctcatt attagcatct aaataggcgg tatttagatt aaccacaatg ccattgtccg
    27121 gatccgtttt caggccgttc caggtcatgc tcataccata gccggtacga tcggactgat
    27181 cgacaaaatt acccttgcta tcggtataac cataagcatt atttaccacg ttcgcttcca
    27241 tcgctgcgga aacggtgaat tcttccgtcg gcgaccaggc aattactggg cgcagataag
    27301 caacattttt ctgctgctcc atatcgcgtc catgataatt accgtcatta tataaagatg
    27361 ttccgtcttc aagtaacgtg tttaattcaa aataccagtt atcgagttgt ttgctgacga
    27421 ggaaattacc gccagcgtta gagcgcccgc ggccctcttt catcatatag atataaccgc
    27481 tgccatcgtc ataaagatcg ttcgcagtat taccggaatg ttcaacaaag gtatcctgat
    27541 tcaatgggaa catatcgtag gcttcaaaac ggcctacttt gaccttccag tcgttttctt
    27601 tgccaaagaa gaataccgca tcatcgatat ttactgaacc gtgcatatca cccagcggtt
    27661 gtgcggagaa cccggcgaaa taaccgttgt ccatctttcg cataccgtca aaacctaaca
    27721 gaatacgacc attgagattc cactgttcat tagtgggatc gctattcaca tcagcgttgg
    27781 tcattgccag caggccatga ttactttccg catccatatt aaattctacg tcgccgtaga
    27841 tttttaaatc gccataacct gaaagcaaaa gattaggtga ggtggcattt ttctttgcag
    27901 attccgccgt tgcctgtcca tttaccggcg tattgccctg agcggcctta atttcgcgga
    27961 tctcagcggc ttgttgtgtc tgcagctgtt taacctggct ctctgctttt gccgcccgca
    28021 tttctgccgc ttccaggcgt tcttccagca atgccatgcg ttgttccagc attaaatgct
    28081 gtgtttttgc cataccggta ctacatgcca ataataaacc gatggctaaa gataatgtcg
    28141 attttttcat tatttacgtt ccctgaaata aaggagaccc tacgctttcc aaaaggaata
    28201 cgtatataga gaaatataag gtgtgcactt tcgaattttt aatccaactt cagcaggccg
    28261 atggatagta tcccgccgca taatatgagt aaatccgctg atttcctgga acgtgaggaa
    28321 tataacaata gtgccgtttt taactaaata tcgtaaaa
//
LOCUS       DAZFKY010000047        28262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFKY010000047 DAZFKY010000000
VERSION     DAZFKY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHKCL_001791"
     CDS             complement(<1..209)
                     /locus_tag="ACHKCL_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5762773.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKCL_001792"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKCL_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5762774.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKCL_001793"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKCL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5762775.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKCL_001794"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKCL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5762776.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKCL_001795"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKCL_001795"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5762777.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKCL_001796"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKCL_001796"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5762778.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKCL_001797"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKCL_001797"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5762779.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKCL_001798"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKCL_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5762780.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKCL_001799"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKCL_001799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5762781.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKCL_001800"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKCL_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5762782.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKCL_001801"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKCL_001801"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5762783.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKCL_001802"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKCL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5762784.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKCL_001803"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHKCL_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5762785.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKCL_001804"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKCL_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5762786.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKCL_001805"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKCL_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5762787.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKCL_001806"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKCL_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5762788.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKCL_001807"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKCL_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5762789.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKCL_001808"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKCL_001808"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5762790.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKCL_001809"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKCL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5762791.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKCL_001810"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKCL_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5762792.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKCL_001811"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKCL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5762793.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKCL_001812"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKCL_001812"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5762794.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKCL_001813"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKCL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5762795.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKCL_001814"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKCL_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5762796.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKCL_001815"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKCL_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5762797.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKCL_001816"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKCL_001816"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5762798.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKCL_001817"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKCL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5762799.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKCL_001818"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKCL_001818"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5762800.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKCL_001819"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKCL_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5762801.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKCL_001820"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKCL_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5762802.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKCL_001821"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKCL_001821"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5762803.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6833 a         7590 c         7135 g         6704 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcta ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gt
//
LOCUS       DAZFKY010000048        28165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFKY010000048 DAZFKY010000000
VERSION     DAZFKY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(15..1412)
                     /gene="ccp"
                     /locus_tag="ACHKCL_001822"
     CDS             complement(15..1412)
                     /gene="ccp"
                     /locus_tag="ACHKCL_001822"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5762804.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1817..3466
                     /locus_tag="ACHKCL_001823"
     CDS             1817..3466
                     /locus_tag="ACHKCL_001823"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5762805.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3517..4119)
                     /locus_tag="ACHKCL_001824"
     CDS             complement(3517..4119)
                     /locus_tag="ACHKCL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5762806.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4639..5538
                     /locus_tag="ACHKCL_001825"
     CDS             4639..5538
                     /locus_tag="ACHKCL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5762807.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5587..6600
                     /gene="yhjD"
                     /locus_tag="ACHKCL_001826"
     CDS             5587..6600
                     /gene="yhjD"
                     /locus_tag="ACHKCL_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5762808.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7012..8334
                     /locus_tag="ACHKCL_001827"
     CDS             7012..8334
                     /locus_tag="ACHKCL_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762809.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8516..10576)
                     /locus_tag="ACHKCL_001828"
     CDS             complement(8516..10576)
                     /locus_tag="ACHKCL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5762810.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHKCL_001829"
     CDS             complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHKCL_001829"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5762811.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHKCL_001830"
     CDS             11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHKCL_001830"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5762812.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12670..14166)
                     /locus_tag="ACHKCL_001831"
     CDS             complement(12670..14166)
                     /locus_tag="ACHKCL_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5762813.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHKCL_001832"
     CDS             complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHKCL_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5762814.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHKCL_001833"
     CDS             complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHKCL_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5762815.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17926..21389)
                     /gene="bcsC"
                     /locus_tag="ACHKCL_001834"
                     /pseudo
     CDS             complement(17926..21389)
                     /gene="bcsC"
                     /locus_tag="ACHKCL_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            complement(21371..22477)
                     /gene="bcsZ"
                     /locus_tag="ACHKCL_001835"
     CDS             complement(21371..22477)
                     /gene="bcsZ"
                     /locus_tag="ACHKCL_001835"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5762816.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22484..24841)
                     /gene="bcsB"
                     /locus_tag="ACHKCL_001836"
     CDS             complement(22484..24841)
                     /gene="bcsB"
                     /locus_tag="ACHKCL_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5762817.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24852..27470)
                     /gene="bcsA"
                     /locus_tag="ACHKCL_001837"
     CDS             complement(24852..27470)
                     /gene="bcsA"
                     /locus_tag="ACHKCL_001837"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5762818.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27467..28165)
                     /gene="bcsQ"
                     /locus_tag="ACHKCL_001838"
                     /pseudo
     CDS             complement(27467..28165)
                     /gene="bcsQ"
                     /locus_tag="ACHKCL_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6632 a         7814 c         7106 g         6613 t
ORIGIN      
        1 ggcgatcaaa ttaattattg tttatcctgc atatacggcg tgtaaacccc gttcagactg
       61 tgcaggaaag ctacgatatc atccacatcc tcctgcggca gctctttgcc tacctgatag
      121 cgcaacatca gtttcaccgc cccgtccagc gtcggcacgt caccgcgatg gaagtacggt
      181 gccgttaaag caacgttacg taaaccgggt actttctgac gcaatttatc acgctcttct
      241 ttagtcacat tcatacgacc aatatccgcc gccgtaattt ccccaaagtt aaagtctttt
      301 ttcagcccca acggttcgaa ggaacgtccg ccgagaataa ttccaccatg acaagttgca
      361 catttattat ctttaaataa ttgatagcct tttttctgtt gcgccgtcag cgcattttca
      421 tccccacgca accatttatc aaatggggaa tccggcgtaa ttaatgtttt ctcaaattca
      481 gcaatggcat cagtaatatt ttcgccactg aaaccttgcg gatagacgtc gaggaactgc
      541 gctttaagct gcggatcttt ttccagctta gcaataattt cgtcccagga ttttgacgcc
      601 atttcaatcg ggttcaacgg cggtccacca gcctgatcct gcaatgttgc cgcacgacca
      661 tcccagaact gctcaacgtt aaatactgag ttaaataccg tcggcgcgtt aatcggccca
      721 actgcgccac caacaccaat cgatgttttt ctgccatcga cgccccccgc attcaacgca
      781 tggcaatgag cgcatgaaat ggtgctatca gccgataaac ggggatcgtg atacagcgca
      841 aagcccaacg ccactttttg cgcatcggta ggcagttttt gcgggatggg ctgcaccggt
      901 tcattgcgat gctccggagc agtatcattg ctggcgtaat attccgcgcg ctgttttgca
      961 atccaggcca gtatttccgc ccgctcttca tcactcacct tacccgccca gtgtagcgcg
     1021 gtataacgcg ttggcggcat agtttcatac tgcatcaccc attcaatctt attcagatcg
     1081 ctttgcgaaa cgggtttatc agccagcaga gcagcacgca cggcctcgag gttaaaagat
     1141 ttatatccaa gcttaatgtc gtaatccatc aactgtttcg cgccaggaat ataataatag
     1201 gcgggtaatt ctgccgaagg cgtgtggcaa tagtcgcatc ctttttcgcg gagaaagcct
     1261 aaaaccttat tattttcact gacagcagat gcctgaacat cggcctgttt actgcgttta
     1321 ttatcgtggt accacacata accagataac cctaaatagc aaatcgcgac gccagccagg
     1381 ccgatcgcgg taatacgtga gaccattttc attattcttc cctcacggtg gtgagttatt
     1441 gtgacccaga aaattttcca gaatgctgca aacgtctctg cgagacaaag ccatcataaa
     1501 aaacagggag atacgctttt tgataggaat caatcaatta catgaatata aactatcaat
     1561 aagatagcct gtgcctatta cccagatcag gaatggcata tcatgattgc ggcaagagca
     1621 ggattgaatg ttctgaaaat gaaaaacaac acgaaatctg tatttttata aattccattg
     1681 atattagtgc gttatatttt ttgacaggtt aataaacgtg atccaccgca cgctttgtcg
     1741 cccaccaggc ggagcgaatg actaccctta aagaaaagcc cgataattag cgacgaattt
     1801 cggaggttgg atccttatgc tcaatcagaa aattcaaaac cctaatccag acgaactgat
     1861 gatcgaggtc gatctctgct atgaactgga cccgtatgaa ttaaaactgg atgagatgat
     1921 cgaggcagaa ccggaacccg agatgattga agggctgccc gcctctgatg cgctgacgcc
     1981 tgccgatcgc tatctcgaac tgttcgagca tgttcagtcg gcgaaaattt tccccgacag
     2041 taaaaccttt cccgactgcg cacccaaaat ggacccgctg gatattttaa tccgctaccg
     2101 taaagtgcgc cgtcatcgtg attttgactt gcgcaagttt gttgaaaatc acttctggct
     2161 gccggaggtc tactccagcg agtatgtatc ggacccgcaa aattccctga aagagcatat
     2221 cgaccagctg tggccggtgc taacccgcga accacaggat cacattccgt ggtcttctct
     2281 gctggcgctg ccgcagtcat atattgtccc gggcggccgt tttagcgaaa cctactattg
     2341 ggattcctat ttcaccatgc tggggctggc ggaaagtggt cgggaagatt tgctgaaatg
     2401 catggccgat aacttcgcct ggatgatcga aaactacggt cacatcccca acggcaaccg
     2461 cacctattat ttgagccgct cgcaaccacc ggtttttgcg ctgatggtgg agttgtttga
     2521 agaagatggt gtacgcggtg cgcgccgcta tctcgaccac ctgaaaatgg aatatgcctt
     2581 ctggatggac ggtgcagaat cgttgatccc taatcaggcc tatcgccatg ttgtgcggat
     2641 gccggacgga tcgctgctca accgttactg ggacgatcgc gacacgccgc gtgacgaatc
     2701 ctggcttgag gacgttgaaa ccgcgaaaca ttctggtcgc ccgcccaacg aggtgtaccg
     2761 cgatttacgc gcgggggcgg cctccggttg ggattactct tcccgttggc tccgtgatac
     2821 tggtcgtctg gcgagcattc gtaccaccca gttcatcccc atcgatctga atgccttcct
     2881 gtttaaactg gagagcgcca tcgccaacat ctcggcgctg aaaggtgaga aagagacaga
     2941 agcactgttc cgccagaagg ccagtgcccg tcgcgatgcg gtaaaccgtt acctctggga
     3001 tgatgaaaac ggcatctacc gcgattacga ctggcgacgc gaacaactgg cgctgttttc
     3061 cgctgccgcc attgtgccgc tctatgtcgg tatggcgaac catgaacagg ccgatcgtct
     3121 ggcaaacgcc gtgcgcagtc ggttactgac acctggcggg attctggcaa gcgagtacga
     3181 aaccggtgaa cagtgggata aacccaacgg ctgggcaccg ttacaatgga tggcaattca
     3241 gggatttaaa atgtatggcg atgaccttct gggcgatgaa atcgcgcgca gctggctgaa
     3301 gacggtgaat cagttttatc tggaacagca caaactgatc gaaaaatacc atattgccga
     3361 tggtgttccc cgcgaaggcg gcggtggcga gtatccgttg caggatgggt ttggctggac
     3421 taacggtgtg gtacgccgtt taattggttt gtacggcgaa ccataatatt tttacagcca
     3481 gccgctaact tcctgctggc tgtaaaatta tcctcttcag gaggagatat ttaacatcat
     3541 tgccgcctgg gtgcgatttt tcacttccag acggcgatac agggattcca gatgcgcttt
     3601 taccgttccg gtactgatat tcagcgctct gccgatctcc ttatttgatt cgcccgccgc
     3661 taacatggtt aaaatctccc gctggcgggc gcttaacgat ttgagatctt taatgtcctt
     3721 ttccggcgtc gtccgccagt ctccaggcag aaacattatc cccatcgccg cactatttac
     3781 cgccaacgca aatgtctcga cggttgaatc acgaggcaca atggccagca cattaaaatg
     3841 gataacttcc tgtaaccacc gtttattgca gtccgtcgcc gtaattaaca ccttaacctc
     3901 aggaaattgc accacggttt tttgcagcaa ccagtagcaa aactcaccat cctgatcgcc
     3961 atcgagcata actaaggctt cagggtaact ttccagcttt tgccataact cgtctgcctg
     4021 actggccccc tgaatactca ctcctggaat acgctgctgt aaactgattt tcattccatg
     4081 aataaatatt gactgcctgt caaacatgac tatttgcata actgaatctc cacctgaata
     4141 cgttaaaaag acttaagtag tggaagggta ttacccgcga gaaaaaataa gaattcgcca
     4201 tttggcggtg gccattctac agagatggcg cgtagaaaat agttaccgat ataaatagtt
     4261 acagctaaac gcctgaaatt acatgtcgag ggcactattt aaaacaattt tgaggatttc
     4321 cttatattgg tggttagtac gcatgcaatt aaaaatgaaa ttccgcgacc acaagccaaa
     4381 ataacaaatg gcaatgagac aaaaataagc acaaatagcc aatatgtcct ctattcactt
     4441 taaagggaat cgctgaaaaa tacgctctgt ttaaggggat tcacctttct cagaaagcta
     4501 ttccgccctt ttcctgctga gaaatcgcca cattcgtcat gacaacattg tgaaacccgg
     4561 cattagatgt tagaaaaaat aataaacaat gcgatatgcg cgttactgtt tcgctgtgaa
     4621 caacaatcgg tcaaagaaat ggataaaatt cacgcaatgc agttgttcat caaagtcgcg
     4681 gagctggaaa gtttttcccg cgcagcggat ttctttgctt tgccaaaagg aagtgtttcg
     4741 cgccagatac aggcactgga acatcaactt ggcacccagc ttctccagcg caccacgcga
     4801 cgggtcaaac tcacgccaga aggcatgacc tattatcaac gagcaaaaga tgtgttgagt
     4861 aatctcaacg aactggacgg tctgtttcag caggatgcca ccagtatcag cggtaaatta
     4921 cgcatcgaca tcccgccagg aatcgcaaaa agcctgttac tgccgcacct gtcggaattt
     4981 ctctatcagc atccgggaat tgagctggaa ctgagtagcc atgaccgtcc ggtagatatt
     5041 cttcatgatg gttttgattg cgtgatacgc actggcgcgt taccggaaga tggcgttatc
     5101 gcccgtcccc tcggcaaact gaccatggtc aactgtgcca gtccgcacta tctgacgcgc
     5161 tttggttatc cgcaaaaccc cgacgatctc acttcacacg caatagtgcg ttatacaccg
     5221 cacctgggtg tacatccgtt aggttttgag gttgccagcg ttaatggcgt ccagtggttt
     5281 aagtctggcg gcatgttgac ggtaaacagt agcgaaaact atctcgccgc cggtcttgcc
     5341 ggtctgggga ttattcagat cccgcgcatt gccgtgcgcg aagccctgcg tgccgggcgg
     5401 cttattgaag tgttgccagg ctaccgtgcc gagccgctct ccctttcgct ggtttatccg
     5461 cagcgtcggg agctttcccg gcgtgtaaac ctgtttatgc agtggctggc tggcgtaatg
     5521 aaagagtacc tggactgacc gactatactt tttaagaatg accacgacag aaggacaaaa
     5581 gagcggatga cgcaggaaaa cgagatcaaa cgtcccaccc aggatctgga gcacgagccg
     5641 attaagcagt tggataatag cgagaaaggc ggcaaagtta gccaggcgct ggaaaccgtc
     5701 accaccactg ccgaaaaagt ccaacgccaa ccggttattg cgcacctgat tcgcgcgaca
     5761 gaacgcttta acgatcggct gggtaaccag tttggtgcgg ctatcaccta tttctcattt
     5821 ttgtcgatga tcccgatttt gatggtgtcg tttgccgcag ggggctttgt gctggcctcc
     5881 catccgatgt tgctacagga tatcttcgac aaaattctgc aaaacatcag cgatccgacg
     5941 ctagccgcta cgttgaaaaa caccatcaac accgccgttc agcagcgtac gactgtaggg
     6001 cttgtcggcc tggcggtggc gctttattcc ggcatcaact ggatgggtaa cctgcgtgaa
     6061 gcgattcgtg cccagtcgcg cgatgtctgg gaacgctcgc cgcaagatca ggagaagttc
     6121 tgggtaaaat atctgcgtga ttttatttcg ctgattggtt tgttgattgc gctgattgtg
     6181 acgctttcga tcacctcggt tgccggttcg gcgcagcaaa tgattattag cgccctgcac
     6241 ctgaacagca ttgagtggct gaaaccgacg tggcggttga ttggcctggc gatttccatc
     6301 ttcgccaact atctgctttt cttctggatc ttctggcggt tgccgcgcca ccgtccgcgc
     6361 aaaaaagccc tgattcgcgg aacatttctg gctgctattg gttttgaagt gattaaaatc
     6421 gtgatgacct acaccctgcc atcgttgatg aaatccccct ctggcgcagc atttggttcc
     6481 gtgctggggc tgatggcatt tttctacttc ttcgcccgtt tgacgctgtt ttgcgcggcg
     6541 tggattgcca ccgccgaata taaagacgac ccgcgaatgc cggggaaaac gcagccttaa
     6601 aataggccgg atgcagttca aaacgcctta tccggcatac gaatttattc ggcctggctc
     6661 cccgtaggcc gaataagatg cgccagcatc gcatccggct ataatgcgca cataacctct
     6721 tgaaactctt tcccagagcc tcttcagcca tctattcagg agcaaacaat ttcatgccaa
     6781 ctcataaccc cagcatataa atccagttgg taacttttat ttaacctgaa accagtttta
     6841 tccactattt ataaaattat gtgaagcatt tcatagaaga aaaatcactg gcctaaacat
     6901 tatccccttt ttgcctggtt tttgaccatt tccgcgattt gttacacatt gaaatatcac
     6961 ttttgctgtg cgtaatatgg ctattcgtta gccaaaaaat aagaaaagat tatgcaagca
     7021 acagccacaa cactcgacca cgagcaagaa tacacgccga tcaactcgcg taataaagtc
     7081 cttgtcgcct ctctcattgg cacggccatt gagttcttcg acttttacat ttacgccact
     7141 gcggccgtta ttgtgtttcc gcatatcttc ttcccgcagg gcgatcctac ggcagcaacg
     7201 ctacagtcgc tcgccacctt cgccatcgcc ttcgtcgcgc gccccattgg ctctgccgtt
     7261 tttggtcatt ttggcgatcg cgttgggcgt aaagcgacgc tggtcgcctc gttgctaacg
     7321 atggggattt cgaccgtggt gattggtctg ctgccaggct atgccacgat tggtattttc
     7381 gccccgctgc tgctggcgct ggctcgattt ggtcagggtc tgggcttagg tggtgaatgg
     7441 ggcggcgcgg cgctgctggc gactgaaaac gccccaccgc gcaaacgtgc attgtacggc
     7501 tccttcccgc agctgggcgc accgattggc ttcttctttg ccaacggcac tttcttgctg
     7561 ctttcctggc tactgaccga cgagcagttt atgagctggg gctggcgtgt gccatttatc
     7621 ttctcggcgg tgctggtcat tatcggcctg tatgttcgcg tgtcgctgca tgagtcgccg
     7681 gtgtttgaga aagtcgctaa agcgaaaaaa caggtgaaga tcccgctggg tacgctgctg
     7741 accaaacatg ttcgcgtaac cgtactgggt acgttcatta tgctggcaac ctatacgctg
     7801 ttttacatca tgacggtcta ctctatgacc tttagtaccg ccgccgcgcc agttgggctt
     7861 ggcctgccgc gtaacgaagt gttgtggatg ttgatgatgg cagttattgg ttttggcgtg
     7921 atggtgccag tcgctggatt actggctgat gcctttggtc gccgtaaaag catggtaatc
     7981 atcaccacgc tgatcatcct gttcgcgctg ttcgccttta acccactgct cggttctggc
     8041 aacccgattc tggtttttgc cttcctgctg ctggggttaa gtctgatggg tctgaccttc
     8101 gggccaatgg gtgcgctgtt accagagctg tttccgacag aagtgcgtta caccggagca
     8161 tcgttctctt acaacgtagc gtcgattctc ggggcttccg ttgcgccata tatcgcagcc
     8221 tggttgcaga ctaactacgg gctaagtgcg gtggggttat atctggcggc gatggctggc
     8281 ttgacgttaa tcgccctgct gctgacccat gagacgcgac atcagtcgtt gtaataagag
     8341 tgaaaagcct gatgcgctac gcttatcagg cctgaatttc catacaatat attgaattcg
     8401 caaggatttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aagcacactt
     8461 tgccaacaat ctaaatctgt ttaattgccg gatgtcagac atccggcaat tatcttcact
     8521 tcttcatctg cgacaaaatc gtccggcact gattagcctc cccttcggaa ggtgagatca
     8581 gtgccagcaa cgctgccgct ggcgtaacca gcgttgccag tgccgcagca acagcaccac
     8641 gggcaatcaa cggcccggct ttcaccccag cctgaggatt tttaaacgtc ccacgcacat
     8701 acagcggcga acgcagtgtg ataatccgaa tacctttact ctccggatca atagtcaaat
     8761 ccagctgttc cgaagcaaaa cttgccgtgc cggtaacgtt aatcaacgcg ttctcagtat
     8821 cgaaagcaaa aatctgcggg cgtgccacgc cgttggcaat attcagattc gccgccgcgc
     8881 agttcacccg cacctcatcg tcaccaaata tcgcaccgac aatgtagttg ccgacattca
     8941 gcccaacaat ctccatcagg ttgcggctca ccagcccgtc attcatcaac agtttcaggt
     9001 tgccgttact gttgcctaaa agtgccgcca ccgagttacc gctaccgcgc agttccgcgt
     9061 caccgttcat ttcccccagc gtcttctgca tcagttccac atcgggcatc agttctttca
     9121 gtttcagtcg acgagcctga atatctgccc gcccctgcat cggcttttta tcgccttcca
     9181 gatgaatatt cgccgcaatg ctgccacccg caatgccaaa tttcagcggt tgcaggcgca
     9241 agtcagcatt tttgaggatg atatgagtag aaagatcgct aatcggcagg ctactgccat
     9301 gctcaatgcg ccgccctttg aagcgaacat cggcatccat aacgtcccat ttatcggttt
     9361 cgaagcggtc ataaggcagc actttgcccg caggctgaac gcttttttcg cccttcttct
     9421 gttcagaccg tttcgacttt tctgcccctt tcccggaatc aacgccaatc aacggtccca
     9481 ggtccgccag ccgcaattgc cgcgactcga catcaccttc cagttttggt cgtggcttgc
     9541 cggtggtgta gaccagagaa ccgtggatat cgctatcgcc aattcgccca ttaaagccgc
     9601 gataatcaaa gaccgacgat ttttcagtgt cgattttcgc taccagccga ccatccgttt
     9661 caaacggcgg ggtatcgggc agcagaacgc ccgtcagttc atagagatca cccagtgaat
     9721 cgccagaaaa tttaagccgt aaatcgacac cgcccatctt cattgggtca ttcacgacgc
     9781 catcaaaagc aacacgggta ttacctgaac ggaaatcagc ctgtaccgga aacggcgtcc
     9841 cttcgccacg cagcgccagc ataccgccta tttttcccgt acccgtgagc ggttcaccgt
     9901 tataacgtcc ctgcgccttc aggccaaaaa cgtaatcgcc caccttttct ttatccgctt
     9961 tacctttcga tccagtaact tcgctgaacg gcagcggctt gcctaaggga tcaacaaaaa
    10021 tctccagatc cgctttgctt actttgtcat caatggcgat ccgcccttga tcgaaaagaa
    10081 tattatccag ccgaaacgac catgccgacg gctttgcatt cgcgtctttg ttatcatcgt
    10141 tggcgagatt aaacgtccag ttattgttct tttcagagag gcgaatcagg cgcgcgtcgg
    10201 gcttttcgag cttgatccac ggcagccaga ccgttttggt cagcagcgcc agcggggcca
    10261 gcgttgcttc tacgcgtggc aaatgcacca tcgtgacttc gggaatatcc ggtggattgc
    10321 caagaatgat gtcttccgcg tgtacatggg gccacggcac ccagctgcgc cagccagttt
    10381 cttgcttttg ccgctcccac accacgccca gatcgccacg gatagcgaac ggacgattca
    10441 actccgcaga gactttctgg ttgatggtcg gtttgagtcg attccagtca aaagttgcaa
    10501 tcaaaatgat cgccacgaca atcaacaaca agaaagcccc tgaaatcgca gcggttattt
    10561 tgcctgcctt gctcatcgtt cgcttcctca tattcttcct gtgccagtcc taaagatagt
    10621 ccagccaggc ggaaaatgag gcagcttata gcgccagaac cgccgtattc agcgtttcta
    10681 tcggtgccgg gcgtgaaaga aaccagcctt gtgcggcgaa tgcgggcgag ttctgtacat
    10741 cacgccactc ttccggcgtt tctacgccct cgacaatcac cccgcgacaa tagcgattca
    10801 ttagatgtaa aagctgagaa aagagtgtgc gtccttccgg cgactgacgc agcatcacaa
    10861 acagttctcg cgcgattttg atgtagtcat aacgcacttc acttagtgca gagaaatttg
    10921 ccatcccggt accaaaatca tccagccaca gcgggccaaa ttcacacatc gaggcaaagg
    10981 ttgaatcttt cggcagacgg atatgctcca ccagttcgaa acgcagccag ggaagacgct
    11041 caatctggcg caggattttt ggttgctgac gcagggcgat gagcgtagga ccatcaatat
    11101 tgaccgatgc cagcaggccg tgctctataa agaagtcggc tttttgcgcc agcaaatcaa
    11161 tctgctcttt cacaacctcc atccgatggc tgacggtgat ttcagtaaaa tagcgatccg
    11221 gcggcaggcg ttgcgaaggg ttcaagggat gcgtgaccgc cgttaatagc tccacggcca
    11281 ttaaccgccc gcatgtttga tagatcggct gccaggtgta agcacgctca cactgcaacc
    11341 aaaaacgccg ttcctgcaag ctctcgatgc ttgcttcagg gttgcttatt cgctggataa
    11401 cctgccttat catcaaagat gtcctgtgtt taaaagtgat gcagcccgga ctcgacaaag
    11461 attatcggcg cgcaagggca gaactttacc gcctggccgg ggtaaatttc cccactccac
    11521 aacggaatct ttcaggaata cgttctggct caaaaaaata atggaacact gttttaatat
    11581 ggttgaccag caaaccacca cagcgcaaac taacgctaat tttttacaga tcaggttcac
    11641 gactatgtcc aaaaagattg ccgtgattgg cgaatgcatg attgagcttt ccgagaaagg
    11701 cgcggacgtt aagcgcggtt tcggcggcga taccctgaac acttccgtct atatcgcccg
    11761 tcaggtcgat cctgcggcat taaccgttca ttacgtaacg gcgctgggaa cggacagttt
    11821 tagccagcag atgctggacg cctggcacgg cgagaacgtt gatacttccc tgacccaacg
    11881 gatggaaaac cgtctgccgg gcctttacta cattgaaacc gacagcaccg gcgaacgtac
    11941 gttctactac tggcgtaacg aagccgccgc caaattctgg ctggagagcg atcaatccgc
    12001 ggcgatttgc gaagagctgg cgaatttcga ttatctctac ctgagcggga ttagcctggc
    12061 gatcttaagc ccgaccagcc gcgaaaagct gctttccctg ctgcgcgaat gccgcgccaa
    12121 cggcggaaaa gtgattttcg acaataacta tcgtccgcgc ctgtgggcca gcaaagaaga
    12181 gacacagcag gtgtaccaac aaatgctgga atgcacggat atcgccttcc tgacgctgga
    12241 cgatgaagac gcgctgtggg gtcaacagcc ggtggaagac gtcattgcgc gcacccataa
    12301 cgcgggcgtg aaagaagtgg tggtgaaacg cggggcggat tcttgcctgg tgtccattgc
    12361 tggcgaaggg ttagtggatg ttccggcggt gaaactgccg aaagaaaaag tgatcgatac
    12421 caccgcagct ggcgactctt tcagtgccgg ttatctggcg gtacgtttga cgggcggcag
    12481 tgcggaaaac gcagcgaaac gcgggcacct gaccgcaagt accgttattc agtatcgcgg
    12541 cgcgattatc ccgcgtgagg cgatgccagc gtaaacgcag catatgcaaa aggctggata
    12601 aagagttacc cctctatcca gctcatccat aaatacctga tggttgtgct aactcatcac
    12661 catcaggcat tactgtgcag gtggaatatc cgtcacttca ggatgtacgt catccgtggc
    12721 gacagaggtg gtcgcagcgg cggtagatgg ggccatgatt tgatcccagg ccgcctgcaa
    12781 cgctttcatg ttaaattccg gctcgccttt cggctgcagc agtattaacg ccatatcatt
    12841 cgacaactgc tgacgcagat cctgatttaa catctccaca gtcaggctgt taaggaaatc
    12901 ctgccgcagt ttctgatact gctccggcgc gatatcgacg acctgatttt gcaacgaacg
    12961 catccgctgg cccatcagaa tatcggtatc agcccgtgca taggcggcaa acagtttctg
    13021 cagctccagt ttcttttgcg ccactaacgc attgaactct tcttccggca gacctttatc
    13081 gcgaaccttc gccagttcac gcgccaccag attaaggttg ctgttcagct tgtcgtttgg
    13141 tgattcgatg ttgatggcac actgcgcacg cagatacagc acacggcagt caaatccaag
    13201 accgatgtct ttgctgttac tggcgcttaa cgcttgctga acatgccaga acagcgcctc
    13261 acgggccagg tccgcacgcc agtagcgcag cagtgcggct gattcacgaa tcggctgcca
    13321 cggcgtatcc cacatgatag ataaccggtc ctgacgcacc gcgtcagtca taatactcac
    13381 cgcttccgca cgcagcggag aaagcgtcgg caccggagcc ggtgtttcac gtttgccttt
    13441 cagttcgcca aacgttttgt tgatttggtc gacgaccgag cgcgcatcca cgtttcccac
    13501 caccagcagc gtcattgcat ccggggtgta ccatttctga tagaaatctt taattttttc
    13561 cgcttcaacg ggttgtttca gcggatcggc aggatcgtga cctaacaagg ttgacccttt
    13621 cagacgatag cgccaccagc cctctttagt atcggcaggc caggttgcca ccatgtcctg
    13681 actttgcagc gcgtggttga tggtttctgg cgtgatagtc agtttgccag tggcatttgc
    13741 cagataagag agcgcctctt tcagcaaatc gttacggtta ttgggcaaac tcagattaaa
    13801 cagcgtggtg tcataagaga caattaccgg cggcatcggg cgtttagggt cgatcccctg
    13861 ctgccacaat gaacgcgcct gtgctgcgtc aaggccaccg ctttgcgtta gcgcaatacg
    13921 agggatggcg tgactgtaac cgctctgttg tgtactttcg gcgagcgaac cggtattaac
    13981 cagcaggcga atttcaacac gatcgctggg acgctggggg gtggtcagca cttgccactg
    14041 taaaccgttg gaaagcgtcc cctgttgcca tgctggatca ggctggagcg catctgcctg
    14101 cacatagcca gcagtggcca tcatcagcaa accgcccgct aaaagtcgaa tttttgtgcc
    14161 ctgcatgtga acccctgatc aactatcctg gtaataaaaa aactgcccgt tgtcggtcag
    14221 tctaaatgac gttaaaaaca cttcgtgtta gaccgcaaga acatgaaaat gtcacggaag
    14281 aagtgaaata aacctgaact cacccaggtg gtaagttcag gcacaggggt caattatgcg
    14341 caaacacccg cactcgggga agggagtgcg ggcataagtg atgagattaa gaggataatt
    14401 cgtgcgtttt gccatccggc gcacgattat tcagcgcatc gtccagtttt ttgtggtcca
    14461 gttctttcac ccacttagca acgacaatgg tcgctacgcc gttaccgacc aggttagtca
    14521 gcgcacgagc ttctgacata aagcggtcga taccgaggat cagcgccaga cccgctaccg
    14581 gcaaatggcc caccgcagag agcgtcgccg ccagcacgat aaagccacta cccgttaccc
    14641 ctgccgcccc tttagaagaa agcagcaaca cgattaacag cgtgatttgg tggacgatat
    14701 ccatctgact gttagtggcc tgggcgataa acaccgccgc cattgtcagg tatatcgatg
    14761 tgccatcaag gttaaacgag tagcctgtcg ggatgaccag ccccaccacc gatttacggc
    14821 agccgagttt ctccatcttg tcgagcatac gcggcagcgc cgactcggaa gatgaagtcc
    14881 ccagtacaat cagcagttct tcacggatgt agcggataaa tttgaagata ctgaaaccag
    14941 tcgctttagc gattgaaccc aataccagca ccacaaacag gatacaggta atgtagaaac
    15001 agataatcag ctgccccagt tgcaccagtg tgccgacgcc gtatttaccg atggtaaacg
    15061 ccattgcccc gaacgcacca ataggtgcca gacgcatgat catattgatg atgccgaaga
    15121 tgacctgcga gaaactttcg atgacgttaa aaatcagttg gcctttgctg cccagacggt
    15181 ggagcgcaaa accaaacagt acggcaaaca gcagcacctg cagaatgtta ccgctggcaa
    15241 atgcgccaat gacgctcgcc gggatgacat ccataatgaa ggcgacaatg ccctggtctt
    15301 tcgcctgatc ggcgtaaacc gctaccgctt tcgcatcaag cgttgccgga tcgacgttca
    15361 ttccggcacc aggctgcacg acgttaacga tgataagacc aataatcagc gcgatggtac
    15421 tgacaatttc aaagtaaagc agtgcgactg cgccggtacg accgaccgcc ttcatgcttt
    15481 ccatgcccgc aatgcccgtt acgacggtac aaaagatgac aggagcgatg atcatcttaa
    15541 tgagcttaac gaagccgtcg ccaagcggtt tcatttgctc gcctatttca ggatagaaat
    15601 ggccaaggag aataccaatg gctatcgctg tcaggacctg aaagtaaagg cttttaaaca
    15661 gagaggtttt catagggtgt cctttagtaa aaccacaggt cttgtaaggt tatggggtac
    15721 ctgcggcctt aaaataacac ccagacaaca tcacagaaat gtacctggat cataattgaa
    15781 acaaaaaggt taaaaagttt gagctggctc gcacaaacca gcacttttta aagttttgta
    15841 atcagtttgg ggtagctact tttcttccag gtaactctct tcgaagattt caatagggag
    15901 tggacgagca aaaaggaagc cctgggcaat accaacgccc gcttttgcca gccagtcgcg
    15961 ttgtgcttca gtctccacgc cttcggcaat catttgtaag ttcaggctct gcgccagcat
    16021 gataattgca gcaatcatgc tgctatcttc cggcaagcct tcaacaaaca ttttgtcgat
    16081 tttcagaacg tcgattggca acgatttcat atgctgcagc tgacgcagcc ctgcgtagcc
    16141 catgccgaaa tcatccagcg ccacccgaac tccggcatta cgcagcggac ggaggattgc
    16201 caccgcagca tgagggtcgt caatacgtcg gctttctgtc acttccagaa tcagtgttcc
    16261 cggctgaatg cgatagcggg ttaacagttc cagcatatcc gccaccatat tcgggtgcat
    16321 cagttgcagc acagagaggt ttaccgacaa gggcagcata atgccgcgct cttgccaggc
    16381 tgcaagcaat cgacaggact cttccagcac ccagtgaccg acggtaacca tcagcccaca
    16441 gcactcaatg cgatcgatta agccatccgg caggtcccaa ctgccatccg gttgctggat
    16501 acgcagtaac acttccgcac tgaccagttt accgctggtc atctcgacct gtggctgtaa
    16561 ccaaatagca aactgatgat tttccagtgc attaaggata tcgctctctt ccgtcaaccg
    16621 cttctgggcg gcttccatct gctgcggatc aaagaactga atctgattct tgcctttatg
    16681 gcgagcggta aatgccgcag aaatagcgcg actgtaaagc tgttcggcgg tgagatcgcc
    16741 gtagaacatc gccacgctaa tgctacagtg cggacggagt tgaatacgtt caatcggcag
    16801 gcgctcgctc atgatagtga gcacttgctg acccaaagtg attgcgtgcc acggttcatg
    16861 tacaccgttg gcaatgacag caaagtcata accgctaatc tgcgcgagga tcatacgtgg
    16921 cgacagtacc gatttgagtt tttccaccag cgtcagcagc agaatttctc gttgcgcctc
    16981 tttcagcacg cccgcagtat cacgcagagt ttcacaggtg atgatcatca gcgcggtggt
    17041 ttgtttacgc gcgacaacct gctccagcat ctccatcagc aaggctttgt tcggcaaatc
    17101 cgacaccggg aagcgcatcg cattttcgtt ctgttcttca taatggcgct gcagcaattg
    17161 ctggttgagg ttgtaactgc gcaccaacat accgatttca tcgtcctgat gcagacgcgg
    17221 taatgccagt tggtgaccaa caagctcctg ggctgggatg gcgttaagtt cgcgagcaat
    17281 attgcgtaac ggatgcaaaa tcaggcggtt aatgcaccag ctaatcgcca ccgtcaatat
    17341 taacgacaaa agtaagtaaa tggtcactaa cgttgagagg gtgctcatca cgaacttata
    17401 catacggaag gaatccgcct gtagcaccag ataggcaatt ggctgcggat ttgccggacg
    17461 ttcgagcgag tagacgccca gcgagatttg aaccggtagc tcaaacaggc gagtaaccat
    17521 taccggcacc ggacgctctg gaataaaact tttacgcagc gcctggaact ggttaggcag
    17581 cactacatcg gcacggctga ccacgcctgc cggtttaatg ctggcaagaa tagcttccgc
    17641 ttcgggaata tcacctttca aaatggctga agataaaggt tcgcggacag agcgagcaat
    17701 gctttccagt tgcgtagccg tgttatagcg attctgctgg accagatgga acagcaaaac
    17761 ggtgcaaaaa ataaaaacga acaccaggac aacggctgcc accattgcca tctgcttgat
    17821 tgttaacgag cgacttacgc gcaaattgtc tccccccgga aatcccagat tgccgcccga
    17881 gtatacccga tcgcagcggc attaagagag gcgctatctg aaaacttacc agtcggcgta
    17941 aggtatcagc ggctgcggcg gtaaatccat gtcaccctgc catccggcgg cggaataacg
    18001 tacgtagagc aggaaatggc tgggggcgta atctttcgcc tgctggatat caattgccgt
    18061 gccgacaaac cagttggaag taacacgtcg ttcaagtaat gcccgcgccg tatagccgaa
    18121 gccctgactg ctgccgccat cgttggattg ccgcgcagct tcttcctgcc agtcggtcgg
    18181 gatcagattc atcagcggat aacgcggcat ggttttggtg cgtgaatgcg accacgagcc
    18241 agacgccccc agctcccacg accagttttc cgtgcgctcc cgccacatca ccggtatggc
    18301 aaacgacagg tattcctgcg ggctgtagta accgccctga ccgagtgagt agccactcag
    18361 gtctttgtcg taatgccaga tcatgttgtt caggccgatt gtgacgcggc gattgttctg
    18421 gttgatgacc ttatagtaat agcccgtcat ccagcgcaca cgccagttat cttcgacatt
    18481 tttaccggtt aactggtcgc cactaagcga tgcccagacg ccgtttgctt cacctttatc
    18541 gtagctcagg cttagcccca cgccgtcggc acgcacgccg ccccattttt tcccggtatt
    18601 gctcgggaag tctttttgcc caccaaaggc cagcaaagaa ctggagatgg gacgacggtg
    18661 ggcgttaacg gtgtaaccca gcggcccgat atcatcgctg taactgatgc cgccgaccac
    18721 atccaccacg ttgaagccca tcggcgtggt gccgatatcc cagctccaga cgtcatttcg
    18781 ccagccgacc gccacgctgg caccggaatc cgactggctg cggttgccgc tacagtcccg
    18841 taatgtacag gtgccccagt tgtcatccca tttgccatcg gcattagtgg agaaactgcc
    18901 gacgttcata ttgacgaaat cactgcgaaa gaacatccgc ccgtcagaat acggcgcatc
    18961 cacctgcaac atggtagtgt gcgctttcag atcggagtaa ccaccggtgc cgctcgaacc
    19021 ccaataatca tgctcgaggg tgacgttaag atcctgctgg cgatagaggt ccgccgcatc
    19081 gctgcgcacg ccgcgtttca gccagtcatc tttctcgtcg ttacgggtca gtcgggtaaa
    19141 ggtgtcgtta tcctgcggac gcgtcgtagt cacaccggat gcgaccatgg cgtctttgta
    19201 ggtttccagc gcctgctttg gatcgcccgc ctgcgcttca aacttcgcac cgtcgcgcag
    19261 caccatcgcg ctttccatcg acggtggctg agattttgcc tgcgggatca acttattaaa
    19321 cgtctgctgc gccgctgcgg tatctccaag ctgcgcctgc gccagcgcca cgcgccgctg
    19381 tgtgttcagc gaggcgttat cagtagcggg cagtttcgcc agctggctac gtgccgccgc
    19441 tgtgtcaccg gcagcaatat ccacttccgt cagaccaaga atggcatcgg cgttagttgg
    19501 ctcccgcgtc aggacattct gatatgcagc gcgggcggcg gtgtaatcac gtcgctgcag
    19561 cgcccagtca gccagcgtga ggtcaatacg cgtggaaggt ggttgctgac gcagcatcgc
    19621 ttccgcttca gcttctttgc cgctttctcg caggcgatta gcggtttcca gcacctgatc
    19681 gctttgcagt cgattaacca gctcctgaat attgctgctc cactgcgcac gcggcaggct
    19741 gttgatatgc gccagcgccg ctctgtcctg gtcatgacca gagaggtaca gcccgtaagc
    19801 gtaaacctgc tccgggtcgt tcggcttctg ctgcgccaga ttgcgcatta acgtatcggc
    19861 ctggctgcgt tgtccggcct gccagagatc ctgcgaaagt cggtaagtaa tccatacgct
    19921 gccggggtcc agcgccagtc gttgccgctg aagtgctgcc gcctgcgccc atttgccctg
    19981 gttttccagt gcctctgcct gctgtgccag acggtcgttt tgcaggctgc gttcgatatc
    20041 atcaatgcta cgccgctgac tggcagagag tgaggcgata aacgcttcag ctttttctgg
    20101 cgattgctgg cggtaaatat ttgccagccc gcgcacggcg ttagtgttgc cgctgtccat
    20161 acgcaaggtc tgctgataat aacgttcggc ggcgggataa tcttttcgcg ccatcgccac
    20221 atcgcccagc cccagcactg cataactgtc ggtgttatcg acattacgcg cctgctggaa
    20281 caggcgttct gcccggtcag gattattggc tttcagcgca gcatcgccct gctggatcgc
    20341 cagccagtag cggtttactt tcagcagact gttccatttg tcgttgttgc tgctgtgcgg
    20401 atccagtgcg agggcttttt ccagattcgc cactgcattg gcgcgatcgc ctttctgtga
    20461 atatgcctgg cccagcgccc ccagagcttc actgtctttc gggtttgccc gcactgcctg
    20521 ttgtagttcg ggaatggctt tacccgccat accagagtcc accgccgcta aaccttgcgc
    20581 acgagcgcgg aaagcaggat cggccagctg tttttgctgt tctgccagtt gcgattgcgc
    20641 agccgccacg ctatcgccat cactaaagat agaaagatat tttttcagcg ccgacacact
    20701 ggcatcactg acgggcatgt ctttaatctg cccgtaccag attttagagg ccccttcgcg
    20761 cccggcgttc gatttcgcca tctgttccag gacggcaaaa ccttcgtcac ggcgatcgct
    20821 actaaacagc aatagcgcca gattgttttg caggcccgta ttgcccggtt catccgcatt
    20881 gatgcgtttt aactgattaa tcgcttcgcc acggcgagcc ggaattttcg ccaccgtact
    20941 ccagtactcg acagcaatgt caccttccgg cggcgcaccg ttgaacagtt cgccacagct
    21001 tcttccgcat gaccggtagt cgcctgcaat cttgcctgtt gcagtgcctg acgaccatcc
    21061 ggcgtggaaa gtagcatcgt agtccgcgac gatttatacg catttgaact cggcgctaac
    21121 tgcgacagcc gatcgagctg tttttgcgcg ccatcaatat cgccctgacg taacaaagaa
    21181 cggaaacggg cggcaacgac gtccgggtta ttcggatcaa taagttccag ccgatacaac
    21241 gactgctgca ccaggtcttc acgatgggtc gcttcgccta accgaacttg ctccagcaac
    21301 tgttgctgag cggttggtgc tgcctcgacc atcggcatga cggccagacc gagggaaagc
    21361 gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat aactcacctt
    21421 ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc agcacatagt
    21481 tgtaataggc atcgctgccg ggaaagttat cggccacgcg ctggcgctga acggcctgcg
    21541 catcacggtt ttgtaaaaag ggcagcatgg cggcagaaaa accgaccggt cctttaccct
    21601 gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc tcagtgaatg
    21661 tcgccatcgg tttaaaccgg ttgagcatcc gcgctttctg cggatcgctg tcaggcatca
    21721 tgcctaccca catgtaaacg cggatagcgt cgtagctgct gatcaatgtt ttttcggctt
    21781 ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaag cctttcgggg
    21841 cggtttccag caataaacgt tgattggttt cgcgcagcgt agtccacggc gcgccaaagc
    21901 gggtgaaata ctgcgccagc gtcggcggca ggtagctggg gttaaaacgc cagctgttat
    21961 cctcagcaaa acccactttg cccggtaaca acatggaacc cagcccaggc accgtcacca
    22021 cttcctcccg cgcaatacgt tttagcaacg cgctgccgat gtcggtataa cgctgctctt
    22081 tccacaaacg ccccgcctcc agcaacgacc aagccatcca gacatcacca tcggaggccg
    22141 aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc caggcgggca
    22201 aacgttcttt taaagaaccc tgagcgagat tgttctgcgt ccagtcgaga atattatcga
    22261 aagctgcacg gtcgttagcc gccagggcaa agaacatgcc gtaactttgc ccttcagagg
    22321 tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg atgtaatcct
    22381 ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag gcagccaaca
    22441 gcagcatcgt cacgagccca ctacgcaaca cattcatctt caattactcg ttatccgggt
    22501 taagacgacg acggctgata atacgcagca aacgccacag tacccatgcc agcaatatca
    22561 cactgatagc cgccagcacc gccagcaccg ccagcaccgc cagcagaatc ggatggtttg
    22621 ccagcgcata ccacaagcgc tcgaaccacg gcagatgacc tacgtaataa acgtcgccaa
    22681 cacgtaggct gttgataccg gactcgcgga tcaccgcgac cgaaccgaac atggtggcgc
    22741 gtttgccgct atcgttcacc gcatcgttaa gcatttcata accgcgtggg ctatctgcca
    22801 gcagcgcaat cacgctgcgc tggtcgttat acggcgactg gaagccaatc accgccgcca
    22861 tcgcaccgga agaggtcagc gttgactggg tttctgccgc gcgatcgctc tcgtccggca
    22921 caatgccggg gaacggggtc tggcgcatcg gtgttttcac ccagctttcg gtcgcctgca
    22981 ccaataggtc gatctgctta tcgtctttca gtttgtccgg gataccaccg atgatcatga
    23041 tgtcggcatc tttgccctga atggtgctgc catcatcggt caccgtcaga ttaatcgccg
    23101 ggaagcccgt ctgtgcgccg ataaaaccaa cagtattcag caacgtttcc atctgtgctt
    23161 cgttaggcgt tttcggcatc acggtgatgg tttgcgacag atccgccatc cggctgaacg
    23221 ggaagcccgc gttagcaaag gcgcgtagat ccggcatcgg gatgaagtgg taatacttcg
    23281 agaagtcgat ggtggagtcg tcaccaatca ccacatgatt ctgcaccggc tggaaggtaa
    23341 tacagttatc caccgaaccg ccaggcatcg ggttcatata ctcaaagtcg aagcgcagct
    23401 ggttggtcgc gcccagtttc agcgccggaa tagagacatc cgttttgcca tccagcaaac
    23461 cttgtaatac cggaatccgc agcagcaggc ggttcgcctc ctgtttgctg ctcaggttga
    23521 aggattgcag gaactggtta ttcaggctga tatccatccg cgaactgtct ttcaccggcg
    23581 gcatggtgta gcggtaatta atatccatat caatgccggt actgcgcatc aggtagagat
    23641 ctggcggcag gtttagcgaa acattaatcg ctgctggctc aagaccgctg gattgtaact
    23701 gttcttcata ggttttcagt tcaccaaagg tgaccggacg atcggtacgc acccagttcg
    23761 gcgcatcgta cggcttacgc ggtagcagcg gtttcacttc attcactacc acgctttcac
    23821 cacggaacag aatgttaccc tgagcgatac ctttcgctgc ctgcaacagg tctttgtcgt
    23881 cacgaccaaa caccaccagc agtttgacgt aaggattctg cggatggtta atcatctcaa
    23941 tcaccggggc ttttaccgcc ggatgatcgc gcaggaagtc cggccgtttg tcgttggttg
    24001 caaagacaat ggcattacga tccggcagtt ggttatagag caccgggaag ttctgcccac
    24061 gccagccaga acgcgaacca aaccacgagg cgacaatggc agatgcttgt tgcagcccaa
    24121 catccggcgc acccgcaaag accatcggca aggtgttggt gcggttatca cgcgggtcaa
    24181 agaatggcac cgggaagtgt gacaggtcat tcttcacatt cagggtctga taggtcagat
    24241 ccagtccact gctccgccca acatccagcc aaagcgtggt gctggccggg ttttcgcaca
    24301 cgtcctgata atggccgaca aactccagcc gcacacggtt gaagtcggta ataaacagtg
    24361 gattaatggg catttgcgcc agcgtttttt tacccaactg ttctttggtc actggcagca
    24421 cgcccatcag ctcatcattg agataaacct ttaactgcga ctggacaggc agtaacgatg
    24481 gcgatggcgt gtattcgagg ttgagcatcg ccttcgtcac cacttcatcg ctgcgcatac
    24541 caaactcaat gctgccgttc gggttaatgc cacgtagcac catgctgccc ggcggcggcg
    24601 caatttgcgc aaaggtcagc ttcacatcac gcgaaggacc gttctgcgcc acgactggcg
    24661 catcagcgcc ctgcacgccc ggcatcactt gccccacctg cggattttgt tccgtctggg
    24721 cggctacagc tggctcagca ttgatcagtg gttgcgttgc tggcgtcgcc tgcgtcatga
    24781 aagaggggaa cgcactcatc cccatagcca ctgcacaaat ccagaatagt tttcttttca
    24841 tcgcgttatc atcattgttg agccaaagcc tgatccgatg gttgtgccgt ttcgctccgc
    24901 tccgggcggc gcggaataaa cgatacaacc caggaaacca gagaagtcag cacacggaat
    24961 atgcccttca ccgaagaagg cgcaaactcc gccagatggc ggtagccacg gaagccgagc
    25021 ttcagaatat ccagcagact ttccagcggc ttatcttccg ggtagctgtc ctgccagagc
    25081 gcccatgtat ccgcacgggc aaacgtacac tgcacaaaat cgatatgttg ctgggtggtg
    25141 agcggcatta atttcagccc aacttcatta cccatcacgc gcgccacctg ggtcgggaag
    25201 acgtattcct gctgaccgcg tttaagcaac agattcactt tctgcccttc cagaatctgc
    25261 gcctgaccgt tgatcttgat ccccaaacca ccgtcggaga aatcctgaac ggtacacgag
    25321 aagaggtgac catcttcgcg ggcaattgcc gcgggcatcg tcatctccac gcggtgcgat
    25381 cggcgtacct gtttgctttc taccgatacc gcaactgcgc cgccaagaac aatcaggttg
    25441 tagaataccc acaccatact gacgaccacg gtgagcatct cggttggcgg gccatagaag
    25501 tagcgccaga tgcctaccgc aacgcccacc aggttgagca gaacaaggaa gatgtagggc
    25561 cgcgagatca cccagtcgac gtactcttct tccaccagtc cacctttggc ggtgacgtta
    25621 aatttgcctt tgtgcgggtt aatcagcgcc accagcgtcg gtggtgcgat ataccacgcc
    25681 agcaccgttt cgtagatttc actccagaaa gagtggcgat atttgccctg gatcttggag
    25741 ttggtcaggc tggcatggat catatgcggc agcacgaaca gggcgatcat caacgctggc
    25801 gcatagatga tgtaggcatg aagcagcagg aacgccagcg gcgcagtcag gaagatcagc
    25861 cgtggaatgc ccgacaagaa gtggaacatg gcgttgacgt agcacagccg ctgagcaaac
    25921 ttcagccctt taccggtgag cgggttatcg agacggaaga tttgcaccat cccgcgcgcc
    25981 cagcgaatac gctgaccgat atgcgccgac agactttcgg tcgccagccc cgccgcctgc
    26041 ggaatacgca tatacgcgga ggtatagcca cgacggtgca aacgcagaga agtatgcgca
    26101 tcttcagtca cagtttcgac agcaatgccg ccaatttcat ccagcggctt acggcgaatc
    26161 accgcacagg aaccgcagaa gaaagtggcg tcccacatat cgttgccatc ctgcaccaga
    26221 ccatagaaca gcgtgccttc gttcggtgtt ttacgaaaac gccccaggtt gcgttcaaac
    26281 gggtccggcg agaagaaatg gtgtggggtc tgcatcatcg ccagctgttt ctctttcagg
    26341 aaccagccca cggtcatttg caggaacgat cgcgttggta cgtggtcgca gtcgaaaatc
    26401 gacacgaact cgcctttggc atatttcagc gcattgttga tgttgcccgc tttcgcatgt
    26461 tcatgagtgg tacgggcgat atacttcacc cccacgtttt gcgcaaactg gcgaaactct
    26521 tccctgccgc catcatcgag gatccagatg ttcagcttgt ctttcggcca gtcgataccc
    26581 agcgaggcgt aaatggtatt tttcaccacg ttgagatctt cgttgtaagt cgggacaaag
    26641 atatccaccg acggccacag cgacatatct ttcggcaatg gcaccggctg acgattcagc
    26701 ggccatacta cctggaagta gccgagcacc agcacaatcc acgcgtacgt ttcagcgaag
    26761 agcagaataa gcccgcacac caggctgacc ggatcgtccc agttcagcgt agaggtatat
    26821 cgccaccaga tataacggca agaaacagtc agcgacagca caatcaacat tagcgctgag
    26881 aagcgccccg gcatccgccg tacgatcagc gctccccccc acagcagcat caggaagata
    26941 aactgcgcca gcgggttaaa cggctgagta acgcagatta acgccagaat cagcgagaag
    27001 gtgacgatga taccgaggat caaccgccgc gccccggcac tcaaatgacc gagctctttt
    27061 ttctcatcaa gatgctgtgt tttatggcta acgcgctcag gcagctcgtt catccattga
    27121 tggtaacgtc cacgaatatt ctgcagacct gaaaatgccc gcctgcgcgg tttcggcgtt
    27181 tctttgcgcg atgcaccgat cagtaaccag catgtttgaa tgagataacg gaccgggtcc
    27241 agcggacgcg gacgcgaggc gttgatatgc ggatacaggt ttttatgttc tgcgcgaata
    27301 cgctgccagc gcgggtgctc cagtggaata aaaatccagg ccaggatcat ccagaaacag
    27361 ccgagcgtcg cgctgaaagc cgacgcaccg tgacgacgat aatcgcgata acgcccgata
    27421 agccgcgcgt tgaccggcgg gataagcaac caccgggtca ggatactcat gatgcactcc
    27481 cgactggcgt tttcagcccg gaatagttca acaggcacca gttcgccagc gtcaatatct
    27541 cttcagccgc cagcgcatca ctgcgatatt cacctactgg ttgcttagcc gccaggcatt
    27601 cagccatcgc ttcatcacga tgaatgagca tcggcagtaa tcggcgctgg ctttgcaacc
    27661 aaagctggta aatatcgtcc tgaacctgac tgccaatacg gaagttatta atcaaaatat
    27721 gtgcgccatc cggcagcgct tgctgatgca gtcggatatg gcagttggca tccacattga
    27781 cgattgccag cgagtgatcg cacaaactca gcagctggtg ggttatctgc gaggcatcac
    27841 gcggtaagtc gattaaaatc cactggtaac gcccgctggc ttttagttgc tgtaagccgg
    27901 agcaaatatc gctcagccgg gtttgccagt gctgtggatt ttcttgttct tcaatggata
    27961 actgaccaaa aggcagcaaa tcgagctgcg aggtgtagcg caacccagcg tcgcgccagt
    28021 cctagccatc cagcatagct ctggcccagc cctgacggtg ggtaaaatca acattaaatg
    28081 acaggcgcaa caagttgtcc gggcaggcat cgaccaccag gacattttct cccaacattt
    28141 gtaatgacca ggctaatgcg gcggt
//
LOCUS       DAZFKY010000049        27450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFKY010000049 DAZFKY010000000
VERSION     DAZFKY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(112..1704)
                     /gene="lsrK"
                     /locus_tag="ACHKCL_001839"
     CDS             complement(112..1704)
                     /gene="lsrK"
                     /locus_tag="ACHKCL_001839"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO5762819.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIVE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1783..2736)
                     /gene="lsrR"
                     /locus_tag="ACHKCL_001840"
     CDS             complement(1783..2736)
                     /gene="lsrR"
                     /locus_tag="ACHKCL_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5762820.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2985..4520
                     /gene="lsrA"
                     /locus_tag="ACHKCL_001841"
     CDS             2985..4520
                     /gene="lsrA"
                     /locus_tag="ACHKCL_001841"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5762821.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4514..5542
                     /gene="lsrC"
                     /locus_tag="ACHKCL_001842"
     CDS             4514..5542
                     /gene="lsrC"
                     /locus_tag="ACHKCL_001842"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5762822.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5542..6534
                     /gene="lsrD"
                     /locus_tag="ACHKCL_001843"
     CDS             5542..6534
                     /gene="lsrD"
                     /locus_tag="ACHKCL_001843"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5762823.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6546..7568
                     /gene="lsrB"
                     /locus_tag="ACHKCL_001844"
     CDS             6546..7568
                     /gene="lsrB"
                     /locus_tag="ACHKCL_001844"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5762824.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7595..8470
                     /gene="lsrF"
                     /locus_tag="ACHKCL_001845"
     CDS             7595..8470
                     /gene="lsrF"
                     /locus_tag="ACHKCL_001845"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO5762825.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8494..8784
                     /gene="lsrG"
                     /locus_tag="ACHKCL_001846"
     CDS             8494..8784
                     /gene="lsrG"
                     /locus_tag="ACHKCL_001846"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO5762826.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8841..9599
                     /gene="tam"
                     /locus_tag="ACHKCL_001847"
     CDS             8841..9599
                     /gene="tam"
                     /locus_tag="ACHKCL_001847"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5762827.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9603..10517)
                     /gene="yneE"
                     /locus_tag="ACHKCL_001848"
     CDS             complement(9603..10517)
                     /gene="yneE"
                     /locus_tag="ACHKCL_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5762828.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10714..12165)
                     /gene="uxaB"
                     /locus_tag="ACHKCL_001849"
     CDS             complement(10714..12165)
                     /gene="uxaB"
                     /locus_tag="ACHKCL_001849"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5762829.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12392..13810)
                     /locus_tag="ACHKCL_001850"
     CDS             complement(12392..13810)
                     /locus_tag="ACHKCL_001850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5762830.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13949..14308)
                     /gene="yneG"
                     /locus_tag="ACHKCL_001851"
     CDS             complement(13949..14308)
                     /gene="yneG"
                     /locus_tag="ACHKCL_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5762831.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14308..15234)
                     /gene="glsB"
                     /locus_tag="ACHKCL_001852"
     CDS             complement(14308..15234)
                     /gene="glsB"
                     /locus_tag="ACHKCL_001852"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5762832.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15298..16686)
                     /gene="sad"
                     /locus_tag="ACHKCL_001853"
     CDS             complement(15298..16686)
                     /gene="sad"
                     /locus_tag="ACHKCL_001853"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5762833.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16787..17668
                     /gene="yneJ"
                     /locus_tag="ACHKCL_001854"
     CDS             16787..17668
                     /gene="yneJ"
                     /locus_tag="ACHKCL_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5762834.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17746..18861
                     /gene="yneK"
                     /locus_tag="ACHKCL_001855"
                     /pseudo
     CDS             17746..18861
                     /gene="yneK"
                     /locus_tag="ACHKCL_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19012..20202
                     /gene="ydeA"
                     /locus_tag="ACHKCL_001856"
     CDS             19012..20202
                     /gene="ydeA"
                     /locus_tag="ACHKCL_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5762835.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20227..20892)
                     /gene="marC"
                     /locus_tag="ACHKCL_001857"
     CDS             complement(20227..20892)
                     /gene="marC"
                     /locus_tag="ACHKCL_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5762836.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            21104..21538
                     /gene="marR"
                     /locus_tag="ACHKCL_001858"
     CDS             21104..21538
                     /gene="marR"
                     /locus_tag="ACHKCL_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5762837.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21558..21941
                     /gene="marA"
                     /locus_tag="ACHKCL_001859"
     CDS             21558..21941
                     /gene="marA"
                     /locus_tag="ACHKCL_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5762838.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21973..22191
                     /gene="marB"
                     /locus_tag="ACHKCL_001860"
     CDS             21973..22191
                     /gene="marB"
                     /locus_tag="ACHKCL_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5762839.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22222..23121)
                     /gene="eamA"
                     /locus_tag="ACHKCL_001861"
     CDS             complement(22222..23121)
                     /gene="eamA"
                     /locus_tag="ACHKCL_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5762840.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23316..24503
                     /gene="ydeE"
                     /locus_tag="ACHKCL_001862"
     CDS             23316..24503
                     /gene="ydeE"
                     /locus_tag="ACHKCL_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5762841.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(24544..24939)
                     /locus_tag="ACHKCL_001863"
     CDS             complement(24544..24939)
                     /locus_tag="ACHKCL_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5762842.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            25034..26005
                     /gene="ftrA"
                     /locus_tag="ACHKCL_001864"
     CDS             25034..26005
                     /gene="ftrA"
                     /locus_tag="ACHKCL_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFO5762843.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            26113..26208
                     /gene="mgtS"
                     /locus_tag="ACHKCL_001865"
     CDS             26113..26208
                     /gene="mgtS"
                     /locus_tag="ACHKCL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5762844.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            26208..26312
                     /gene="mgtT"
                     /locus_tag="ACHKCL_001866"
     CDS             26208..26312
                     /gene="mgtT"
                     /locus_tag="ACHKCL_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5762845.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26427..27317)
                     /gene="dgcZ"
                     /locus_tag="ACHKCL_001867"
     CDS             complement(26427..27317)
                     /gene="dgcZ"
                     /locus_tag="ACHKCL_001867"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5762846.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         6967 a         6936 c         6612 g         6935 t
ORIGIN      
        1 gatgaatctg caagctggca tttaacaagc cagctctaaa agaagggaaa taagaataac
       61 tatactcaaa aactaacagc cacggtcatc atgatgtggc tgtcaatgaa actataaccc
      121 aggcgctttc cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata
      181 aactgcctgc cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt
      241 gtgcgtccgt tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc
      301 ggcaccgacg ccagctgcaa tggcacatcc taatgcagtg gcttctttga ccaccggaat
      361 attgacgggt aatcccgaga catcagcgag aatttgactc cataatttcc cttttgaacc
      421 tccgcctgca aagactaacg atgaaggatg aatagtcgaa aaatcagcaa tttgctgcaa
      481 gttacacgct gatacaatcg ccgcattttc ttccagcgca cggaacaatg tcgctttgtt
      541 acatttatcc ggatcaatgg acaaattaat aaaggaaggc gcagcgtgat accaggtttt
      601 aaagcgcatt ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact
      661 ggccatctct tccagcagcg tataggtgtc gatgcctaaa cgttcgacaa tcagtttttc
      721 ttcggcacag aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga
      781 ttcagcttgt accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc
      841 tgtcaccggc gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt
      901 ttgtgccgga cgcacaacgc ctaaccccag gcaaccaagc tgcacgtcgc ctcctccaac
      961 gaccaccgga gtgcccacct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc
     1021 cagcaatgtg ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc
     1081 cagcaatgca ggtttccagt cacggatggt tagatcaaga agtcccgtgg tgccagcgtt
     1141 agagggatcc accgcaagtt cgccgctgag catataggcc agccagtcgc tgatcatggt
     1201 gatggttgat gcctgacggt aaatatcgga acgatggtgc gccagccaaa gtaatctggg
     1261 gatggcactt aaagccagtg tttgtccggt cgcgcgataa acttcgtttt caaaggtatt
     1321 gttgtgcagt tctttaagtt cgctaacttc gcgcgccgct ctggcatcca cattggcgca
     1381 ggcccagatc ggtgttcctt cattgttata taaaacaatg ccttcacgca tcgaacatgc
     1441 cgaaacggca gcgatatact ccggggctat gccggcgttg tgcagcgcct ggcgcataca
     1501 ctcacacgcc agttgccagt ttttgttgag atcaaattcc atagaaccag gaacgtccgg
     1561 tactgccaga tgccgccact ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa
     1621 tatcacagcc cgaatacttc cggtgcctgc atccagcgcc atcaggtagt actttgattc
     1681 tgaaggggta aagagtcgag ccattagatt atcctcggct tatgcgttct gtctgttcct
     1741 ctatacgttc tccatcattc ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc
     1801 cgctgctgtg tcctgatcgg taaccagtgc gttgatataa ccgcctttca ttgcagcggc
     1861 aattgcttcg gctttatttt ctccccctgc cacgccaacc cggacgggta tggtcttcag
     1921 cgcgcttaaa ggtaagccaa tcagctcgtt atgtatttta atgtccgtga caacgtcacc
     1981 ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcggc caatcattaa
     2041 ctgttcgccc tggctgatat aaccggagcg aatgatcgtc gcgtcgtctt gttgactaac
     2101 ggccccaatg ccgacaatcg ccacatccgc tgcttgcgcg gctaacagaa catctttgac
     2161 acaattttca ttttttagcg tacgggcaat gtcagcggag gatgcccgca acggagccgg
     2221 aataatattc acactgcacg ccgcattaag ctgtccgata cccgtcatat aagaaccgac
     2281 gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg
     2341 cagcgtattc atggttgcct cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa
     2401 actcatcaac atatgcgccg cgcctatccc cagtcgcccg ccgacatcag catccgcaag
     2461 cccagggatc acccggacat gttgcagcga aaactgacga cgtaattgtg tttcatattc
     2521 cagacagcct tcaaagcgag aattaatctg cacgcgaatg atgccggact gatgcccttt
     2581 ctccagtaat cgcgacactt tcaaacgggt caggccgaga cgatcgctga tctcactctg
     2641 ggtcagcccg tcgtggtagt aaaaccacgc gatccgcgcg acctgttctt cttcacacat
     2701 tccctgttct gaaattaccg aatcgttgat tgtcataatt cattcttcac tttgaacata
     2761 tttaaatctt taatgcaatt gttcagttct tgcgcattta tatctgtgat ggcaaccaca
     2821 gtttgactct acgagcatga acaaacgcaa ccgtgaaaat caaaatagta taaattgtga
     2881 tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa
     2941 atacatttgt tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa acgagtgata
     3001 cccgcgcgtt accgctactt tgcgcccgct cggtttataa acagtattca ggggtcaatg
     3061 tcctgaaagg catcgatttt acgttgcatc agggggaggt ccacgccctg ctcggcggca
     3121 atggtgccgg taaatcgacg ttaatgaaga ttattgccgg tattacctct gctgatagcg
     3181 gtacgctgga gattgggggc aacaactacg ccagattaac gccagttcat gctcatcagc
     3241 tgggtattta tctcgttccc caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa
     3301 acatcctgtt tgggctggca aaaaaacagc tctccatgca gaaaatgaag aacttgctgg
     3361 cggcgctggg ctgccagttt gatctgcata gtctggcagg atcgctggat gtcgccgatc
     3421 gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc gcggattctg atcctcgatg
     3481 aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc
     3541 tgcttgctac tggcgtgggt attgttttta tctcgcataa gctgccggaa attcgccaga
     3601 ttgccgatcg gattagcgtg atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg
     3661 aactgtctac cgacgacatt attcaggcca tcaccccagc ggtacgggaa aaatcgctct
     3721 ctgccagcca aaaattatgg ctggagttac ctggcaaccg cccacaacat gccgcaggaa
     3781 cgtcggtgct gacactggaa aatctgactg gcgaaggttt caggaatgtc agcctgacgc
     3841 taaatgccgg agaaattctg ggtctggctg ggctggtggg tgccggacgc acagaactgg
     3901 ccgagacgct ctatggtcta cgtactttgc gtggcggacg cattatgctg aatgataaag
     3961 agatcaatag attatccact ggagaacgtt tactgcgcgg tctggtttat ctgccggaag
     4021 atcgccagtc atccggactg aatctcgatg cttcgctggc ctggaacgtc tgcgccctta
     4081 ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga taatgccacc ctggaacttt
     4141 atcgtcgggc gctgaatatt aaatataacc aaccggaaca agctgcacgg acattatccg
     4201 gtggtaacca gcaaaaaatc ctgattgcca aatgcctgga agcctcgccg caagtattga
     4261 ttgtcgatga gccgacgcgc ggcgtggatg tctcggcgcg taatgatatc taccaactgt
     4321 tgcgcagcat cgccgcacag aatgtggctg tgctgcttat ctcctctgat ctggaagaga
     4381 tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg cgaaattacc cactctgcac
     4441 tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc
     4501 gtcaggaggc ggcatgctga agtttattca gaacaaccgt gaaatcacgg cactgctggc
     4561 ggtgctgctg ctgtttgtat tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac
     4621 gctgaccatg gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct
     4681 ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt
     4741 gctgttgggg atgttactga acgcaggata ttcactacct gttgcctgtg tcgcgacttt
     4801 actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc
     4861 tgccattgtt gccacccttg gcacgttagg gttgtacaga ggcatcatgt tgctgtggac
     4921 tggcggtaaa tggattgaag ggttacccgc cgaactgaaa cagctctccg ccccgctgct
     4981 gcttggcgtt tcagcaattg gttggttaac gataattctg gtggcattta tggcctggct
     5041 gctggcgaag acagcgtttg gacgcagttt ttatgccacg ggcgataatt tacagggcgc
     5101 tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg gcattttcgt tgaacggctg
     5161 catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac
     5221 cggtaccggg ctggagatga aagcaattgc agcctgcgtg ctgggcggca ttagtttgct
     5281 cggcggttcc ggtgcgatca tcggtgcggt actcggcgca tggttcctga cgcagatcga
     5341 tagcgtactg gtgctgttgc gcattccggc atggtggaat gattttatcg cgggtctggt
     5401 tctgctggcg gtgctggtgt ttgatggacg cctgcgttgt gcgctggaac ttaatctgcg
     5461 gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc gttaaacccg cttcgtcagg
     5521 taaaaaacgg gaggctgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca
     5581 ctgcttgtta ttgagattgt ctcatttggt gcaattaacc cgcggatgtt agatctcaat
     5641 atgttgctgt tcagcaccag tgactttatc tgcattggca ttgtcgccct accgctgacg
     5701 atggtgattg tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc
     5761 attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat tctcctgacc
     5821 ttactgctcg gcgcattgtg cgggctgatc aacgccggat taattatcta taccaaagtt
     5881 aacccgctgg tgattacgct tggcacgctg tatctgtttg ccggaagcgc cctgctgctt
     5941 tccgggatgg ccggggcgac ggggtacgaa ggtattggtg gattcccgat ggcgtttaca
     6001 gatttcgcta acctggatgt gttggggctc cccgttccgc tgattatctt cctgatatgt
     6061 ctcctcgttt tctggctctg gctgcataaa acccatgccg gacgtaacgt gtttttgatt
     6121 gggcaaagcc cgcgcgtggc gctttatagc gcgattccag ttaaccgtac cttatgtgcg
     6181 ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt
     6241 ggttcagcac gttccgatct cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg
     6301 cttggcggtg ccaatattta tggtggttcc ggtgccatta tcggcaccgc cattgcggtt
     6361 ttattagtgg gatatttgca acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc
     6421 agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat
     6481 cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata acccattgcc ataaaggata
     6541 tcttcatgac acttcatcgc tttaagaaaa tcgccttact tagcgctctt ggcattgccg
     6601 caatctctat gaatgtgcag gccgcagagc gtattgcatt tattcccaaa ctggttggcg
     6661 tgggattttt taccagcggt ggcaacggcg cacaacaagc gggtaaagag ctgggcgttg
     6721 atgtgaccta cgacgggccg acagaaccca gtgtttctgg tcaggtacag ttgattaata
     6781 acttcgtcaa tcaaggttat aacgccatta tcgtttctgc ggtttcgcct gatggcttgt
     6841 gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt gctgacctgg gactctgata
     6901 ctaaaccgga gtgccgctct tactacatta atcagggaac tcccgcccag ttgggaggta
     6961 tgttggtgga tatggcggcg cgtcaggtga ataaagacaa agccaaagtc gcgtttttct
     7021 actcaagccc caccgttacg gaccaaaacc agtgggtgaa agaagcgaaa gcgaaaatcg
     7081 ccaaagagca tcctggctgg gaaattgtca ctacgcagtt tggctataac gatgccacta
     7141 aatcattaca aaccgcagaa ggaatattaa aagcgtatag cgatctcgac gccattatcg
     7201 cccccgatgc caatgccctg cccgctgccg cacaagccgc agaaaacctg aaaaatgaca
     7261 aagttgcgat tgtcggattc agtacgccaa acgtgatgcg tccatatgtg gaacgcggca
     7321 cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg caaaatttca gtttatgtcg
     7381 cggatgcatt attgaaaaaa ggatcaatga aaacgggcga caagctggat attcagggcg
     7441 taggtcaggt ggaagtctcg ccaaacagcg tccagggcta tgactacgaa gcggatggta
     7501 atggcatcgt actgttaccg gagcgcgtga tattcaacaa agagaatatc ggcaaatacg
     7561 atttctgatg tgcattactt aaccggagta agttatggca gatttagacg atattaaaga
     7621 tggtaaagat tttcgtaccg atcaaccgca aaaaaatatc ccttttaccc tgaaaggttg
     7681 cggtgcgctg gattggggaa tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg
     7741 taaaaccgtg atgctggctt ttgaccatgg ttattttcag ggaccaacta ccggacttga
     7801 acgcattgat ataaatatcg ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg
     7861 cgggattttg cgcagcgtag ttcccccggc gaccaataag ccggtggtac tgcgggcgtc
     7921 aggtgcgaac tctatcctgg cggaattaag caatgaagcc gtggcgttat cgatggatga
     7981 cgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga
     8041 acatcagtcg atcaaaaata ttattcagct ggtcgatgcc ggaatgaaag tgggaatgcc
     8101 gaccatggcc gtgactggcg tgggcaaaga catggtgcgc gatcagcgtt atttctcgct
     8161 cgcgactcga atcgccgctg aaatggggac gcaaattatc aaaacctatt atgtcgaaaa
     8221 aggttttgaa cggattgttg ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa
     8281 attaccggag cgcgaggcgc tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg
     8341 tgtggatatg gggcgtaata ttttccagtc tgaccatccg gtggcgatga tgaaagccgt
     8401 acaggcggtg gttcaccata acgaaacggc tgatcgggca tatgaactct atctgagtga
     8461 aaaacagtaa ctgcggattt aaggagaaga attatgcacg tcacactggt tgaaattaac
     8521 gttcatgaag acaaggttga cgagtttatc gaagtttttc gccagaacca cctgggctct
     8581 gtacaggaag aaggcaattt gcgcttcgat gtcttacagg acccggaagt gaattcgcgc
     8641 ttttatatct acgaagccta taaagatgaa gacgcagtgg cgttccataa aaccacgccc
     8701 cactacaaaa cctgtgtcgc gaaactggaa tctttaatga ccgggccgcg taaaaaacgt
     8761 ctgttcaatg ggttgatgcc gtgaggcgaa tttatcagtt ttatctacaa ttggggtaac
     8821 gcgctgacgg gagtaaataa atgtctgact ggaacccctc tttatatctg cactttgccg
     8881 ctgaacgatc gcgtccggcg gtggagctgc ttgccagagt gccgctggag aatgtcgatt
     8941 atgttgccga tcttggctgt ggcccaggta acagcaccgc cctgctgcat caacgttggc
     9001 ctgctgccag gataacgggc atcgactcgt ctccggcgat gattgctgaa gcgcgcagtg
     9061 ctttgccaga ctgccagttc gtggaagcag acatccgcaa ctggcaaccg gaacaggcac
     9121 tcgatctgat ttttgctaat gcctcactgc aatggctgcc cgaccactac gaattgtttc
     9181 ctcacttggt ttctttactt aatccgcagg gcgtgctggc agtacagatg ccagataact
     9241 ggctggagcc gacccatgtg ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc
     9301 gcgggcgtga gtcgttggct ggtgttcatg cttactacga tattttgagc gaagccggat
     9361 gtgaggtcga tatctggcga accacctact atcaccagat gccgtcgcac caggcgatta
     9421 tcgattgggt gaccgccact ggattacgtc cgtggttaca ggatctgacc gagagcgaac
     9481 agcagctttt tcttacgcgc taccatcaga tgctgaaaga gcagtatcca ctgcaagaga
     9541 acggacagat actgctggca tttccgcgtc tgtttattgt tgcccgccgt acggagtaaa
     9601 tatcacgtca gttggtaatg gcgatcggga agaacttttg ctggaatttc ggcttcatcg
     9661 ttcatctgta agaggtcaat ttcaatagcg ttacagatgg catccagcgg taaatcattg
     9721 ttttcagtac cgaacggatc ttccagttct tccgccagac aatccagcga aataaaagta
     9781 taggaaatca gcacagagat aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac
     9841 ggcagcatga tacaaaacag ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga
     9901 attggcgtgt aggcaatgcg ctcgcagcct gccagaaccg ctgaaatatc gttaagccga
     9961 tcgttgaggc taataaacag gatatctgaa agttgtccat tgcgtcgctg aaccgccaac
    10021 cactccccca ttattaacaa gatgcggtta gctggagagt tcgaagccag tacacgctga
    10081 agatcttcag tcttgagata atgagccagc acttccacct gtggctgttt gcgtaatgtc
    10141 atgcgtaaac agtgggcgaa ggcgatttgc agccgggcaa actcccttac acttgccgaa
    10201 tccggcaatg tcgtttttac ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc
    10261 caaagtttgc gcgcttcaac gtaacgggcg taaccggcat tattacgaaa accaagaaaa
    10321 atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc
    10381 gtgtaccagg gcagcatgaa aataacagcg atagaaaaga gaaaattgag tagtaagcgc
    10441 gaggatatct tggataatac tgagccatgc cagacaaaaa tacggcgcag ccagtgttgt
    10501 tgtggacgaa caatcatggt tatcttcagg cgtggaaaag tcgccctatt aaacgtgatt
    10561 acgatcacat tctcaagaca ctacttacaa attacctaca aaattacaac attaacaggt
    10621 gctgcatgtg gcacctgtta atgataaagg cttataggat gttgtaacta atataatgaa
    10681 ataagaaacc cggtcgaaac cgggttcaga gtattagcac aacggacgta cagcttcgcg
    10741 catccctttt tcgagaatcg catccaggtc attagccacc tgctcgacca gaccaggcac
    10801 ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac acaatcgcta ccagttcctg
    10861 ggtaccgata acacggtcac gatgctgact ccagagttgc tgataacgtt ccagccagtg
    10921 tgcatcgtcc tgtaccggat atgtttctcc attacgctca ccgcgataga acgcaatcaa
    10981 tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg tttgccttct gccctgccag
    11041 cagctgcggc aggatgcggg tacggaactt ggtcataccg ttgagcgcga tagacagcag
    11101 ctgatgctta atgtacgggt tacggaaacg cccggtgact gcactggcga aagattccag
    11161 ttcatcgcgt ggcaaatcca gtaccggaat aatttcttcg tagatggctt tttcaacgaa
    11221 tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta tccagccctg cctggaacgc
    11281 caccggcacc aacgcggtgt gtgcaccgtt gagtattgcc actttgcgtt ctttatacgg
    11341 tttaatatcg tcaacaatca gcacgttgag cggatatttg tccagacgca gctcggtcgc
    11401 taaggatttc ggtccctgaa tcacaaacaa gtaaaagtgt tcagcggtgt cgaggaaacc
    11461 atcgtgataa cccagttctt cttccagttt agccacttca tcacgcggat aaccggtaac
    11521 gatacggtcc accagcgtag aacagaagct gttagcctga tccagccatt gaataaatgc
    11581 ttctggcagt gcccactctt gcgcatagcg cagcaccagt tcacgcaacg cgtcgccatt
    11641 gtagtcgatc aactcacacg ggatgatgat ccaaccttta tccagcgcac cgttgaaatg
    11701 gctaaaacgt tcgaacagca gacgggtcag ttttgccgga taacttaccg ctggcgcgtc
    11761 atcgaattta tcgcccgcat ggtagctgat acccgcttcg gtggtgttag agaaaacaaa
    11821 gcgcatttcc gggttgtgcg ccagtttcag gaattcatcg tattcactgt agacgctgat
    11881 ttcacgatta acagagcgaa tcagacgcgc gtcgctgacc gcttccccct tctcattcag
    11941 gccacggata atggtggtgt acagaccatc ctgcgtgctc agtgacggcg ggaatgaagt
    12001 ttcaatcgga cgaacaacga ccacaccaga attcagatcg gtgtgctcat tcaggagatc
    12061 tatttgccag tcaacaaagg cgcgcaggaa gttaccttca ccaaactgaa tgatacgttc
    12121 tggatactgt gcaccgggaa aatcgcgacg atttagtgtt ttcacaatgg gttcccttct
    12181 gattagtcat acaacctgtt tgaattggta cgacaggtta gcaaacttta atacgacgaa
    12241 cccctgtttt gatcaactcc tgatgattaa tgagcagttt tatgagaaaa gtgtggcgcg
    12301 gatcatggtt taatcgagga aatacgcctt ttcctggatc ataaagtggt agaacacatt
    12361 gcattcaaat cgcgcggaat gaataaagat gtcagacaac ttcctcaccg taacgcatag
    12421 tgctggtacg gttgcgccca tctttcttcg agcgatacag acaggtatcg gcttcgacca
    12481 tcaatttatt aaagtcatcg gtcagcgttc ggtacgatgc gcgaccacta ccgacgccaa
    12541 tacttaccgt gagataaagc gttttttgtt gccaggtgaa tggttgcagt tcaacgcctt
    12601 tacggatttt ttccgccatt agcagaccat cgacaggatt cactgacggc actgcaacag
    12661 caaattcttc gccgcccatg cgcgccacca gccccttatc accgacaatc ttctgaatat
    12721 gccgggcaaa cacgcttaac actttatcgc cacattcatg tccatagtta tcgttaatac
    12781 ttttgaaata gtcgatatca agtagcatga cagtcagatg ttgtgtctgt ttcagcgatg
    12841 gacttttcag cgcctcataa agaccggacc gtgagtaaac ctgagtcaga aaatcaaagt
    12901 cggctcgcag cgcaacttgc ttcattagcg aattgatcgc tgccacgcta aaagaaacca
    12961 taattgggca tatcgccatc gtggcaatac cgagacgtgc ggagaacatt tgcggaatgg
    13021 agaacggcga accgaccgag atatcaatca ccgaattagc caccagtacg atttccaccg
    13081 caccggtgac aaaggtcaac agacatgtta cctgcggcgt atagcgcact gcacaccaga
    13141 ttaatgcagg cagcgggaac gccagactcc ccgctccgcc aatgactacc gaggcaataa
    13201 ccgacacaat aagcgcgatg gcgggcatca tctgttctgc tttaaagcgc ggcagcactc
    13261 caggaatagc caacgtcagc atacaaggca cgatcaacac gcccgttgag aattgctcac
    13321 tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc gaaccaatcg
    13381 ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc cgtaaggcac
    13441 ttactggctc atatttattt ttccccaaac gcttatcccg cacgaccagt aaggcgacgg
    13501 taacaataaa catcatattg gagaaattga tagccagtga aaccagtccc cattcggtgg
    13561 ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc agtcgattaa
    13621 gccagacata gcgggcaaac acccccgcca ttacgccatt cagcggccag aaaagagaaa
    13681 gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt agcgcaaaaa
    13741 tagcgatgct attacgtagc agatgacctt cctggaacac acggaaagtt gaaatggggt
    13801 gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca acgtaataag
    13861 taaattctca taaaggatag catgttcgct gacagcggcg gcaaataaag gtaccctggg
    13921 aatgctattt tgtcgttttc cgcaattctc atggctgatt catctgtaca accagccagt
    13981 gatagattac cgccacgata taacgttgtt gttcttcgct tagcgaaaca ccatgcggga
    14041 tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc tgagcgataa
    14101 acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat gccggagcaa
    14161 gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct ttctccagac
    14221 aatactgacg ctctttcatg cccaaacgaa agcgagatcg aaattttgaa cgggataaac
    14281 gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact gtttcgtcaa
    14341 ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt ccggactcca
    14401 gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa cgccagattt
    14461 cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac cactggtcgc
    14521 catcagcgcg ttaatttgcc gtgcctgcat tggcgtcacc acaggttcat caatatgaat
    14581 agctttcccc tgattagcca gaaagacaaa cgtccgggcc agctctacac agctcatttt
    14641 cagagcgcag taatgaaaat agttttgcag aacggttgtc acgtcatgat ggaaattgcc
    14701 aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa attcggaacg
    14761 cgctaccacc gtatcgtagg aaatatcaga cacaccgctt aagccgcgca cgacttccag
    14821 catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc
    14881 agcattaata aacggattac gcggaatacc ctgctccatt tccagttgca ctaaagaatt
    14941 gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt cggagtaatg
    15001 acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa aacgttcttg
    15061 cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc ccaatcggga
    15121 accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac caatgagcgg
    15181 ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg ccactttcta
    15241 ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata tgtcgcgtca
    15301 gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga aatgggaaag
    15361 ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca
    15421 ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc tggcctgtgt
    15481 ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag ccagttccag
    15541 cgcatgttct gcatctttcg caatggtgat tgccgcaacg gggccaaaca tttcttcccg
    15601 aaaagcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt agttacctgc
    15661 cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg ttttctccac
    15721 ctgatgatgc agctcatcac gtaaatcgaa acgagccatt ggtccgagag cgttctcttc
    15781 gtcacgggga tcgtccattt tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc
    15841 cgaagcaatt ccctcttcga taataaagcg ttttgccgct gcacaaacct gtccggtatt
    15901 ctgataacgt ccggctaccg ccgctttaac tgccagttcc agatcggcat cgttaagcac
    15961 aataaatgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg ctccagcctg
    16021 tgcaccaata gccgctccag cacgaacact tccggtcacc gtgatagcag caatgcgcga
    16081 gtctttaatc atctgagtga caccgtcatt gtcggcattc agccagccat atacgccttg
    16141 tgggattccc gcatctttaa acacctgggc aatgagctga gcacagccca tcacattcgg
    16201 cgcatgttta agtaagtatc cgttaccagc aagaatgatg ggaacagcgc cacgcatcac
    16261 ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg gtcgatactc
    16321 aataactgcc tgctgatttt ccaccagcgt aggttccgcc ttcagcattg ccggaccatg
    16381 ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg cctggttgat
    16441 tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg cgcgcagagc
    16501 cttaccgata tcacgcagtt tttcagcacg ataatctata tttgtctcgc gccagtcacg
    16561 aaagcctgct gccgccagct gaagtgcgtt ttcgatatca tcagcgccag cccacggcaa
    16621 cacagaaagt tgttcacccg tggcaggatt tatcgaaatt gcatgagttg ccggagtaat
    16681 ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt tactcttgct
    16741 ttaaaatgaa taatattaag ccacttattc acgaatcgag aatattatgg atctgaccca
    16801 actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acgcaggctg cagcaatagt
    16861 gcatcgcgtg ccgtcgaatc tcactacccg tctacgccag ctggaaacag aactgggggt
    16921 agatctgttt attcgcgaga atcagcgttt acgtctatct cctgccgggc ataacttttt
    16981 acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga
    17041 tgagccgcaa ggtttatttt ctctgggttc gctggaaagc accgcagcag ttcgcattcc
    17101 ggccacgctg gcggaattca accgtcgtta tcccaaaatt cagttttcac tttccaccgg
    17161 cccttccggc actatgctgg aaggtgtgct ggagggaaaa cttaatgcgg cgtttattga
    17221 tggacccatt aaccatactg ccattgacgg gataccggta taccgcgagg agctgatgat
    17281 cgtcacgcca caaggtcatg cgccagtaat ccgtgccagt caggttaatg gcagtaacat
    17341 ttatgccttc cgcgccaatt gttcgtatcg tcgccacttc gagagctggt ttcatgctga
    17401 cggtgccgct ccgggaacta tccatgagat ggagtcttat cacggaatgt tggcctgtgt
    17461 ggtcgcagga gcaggtattg cgcttattcc gcgctctatg ctggaaagta tgccggggca
    17521 tcaccaggtt gaagcgtggc cgttagctga gcaatggtgt tggctaacaa cctggctggt
    17581 ctggcgtcgt ggtgcgaaaa cgcgtccgct cgaggcattt attcaactac tggatgtgcc
    17641 tgactcgaca aaacagggtt accaatgagc tatttttgat agttcttgcg gctaatatgc
    17701 tctatatagt gatgttccga tgacttatga ctatatgggg caaatatggt tacgccagta
    17761 agcatcagca attacatatc tcttcctgat gattttcccg ttcgtaatat tgcaccgcag
    17821 gtaaaagaag ttttaaagga ttttattgat gcacttagta caataatatg taatgaagag
    17881 tggcgcacgt ctttaaacat caactcagcc acaaaaaaga tatttaataa tcttgacaac
    17941 ttatcatata ttcagaggac ttcttttcgg ggtaacgaca cgctatacaa tgaaaaggtt
    18001 cagtttaaac tcacttatcc cgccaggaat ggaagacaca aagaaaatat tgaatttcag
    18061 gtagtaataa atttaagccc tatttattta gataatttcc gccatgatgg agaaattaat
    18121 attttttgcg ctcccaaccc aaagcctgtt actatggggc gcgtatttca gaccggcgtc
    18181 gagcgtgtgc tctttctgtt tctgaatgat tttattgaac aattcccaat gatcaaccct
    18241 ggcgttccca tcaaaagagc gcatacacca catattgaac ccctgcctcc ggatcaccat
    18301 accgctgcag attacttacg ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt
    18361 aattttgtca tactcccccg attatggaat aactctgagg ttcacagatg gttcgtcaat
    18421 aaagatccta acttgatcac cgccattctc gacataacgg acagcgaatt aaaagaggat
    18481 ttattgcaaa gcctgatgga ttcattaggt tctaacaaac atgtactacc ctaagtctgc
    18541 atctgttttt tatccctttt agcagaacaa gaatctcctc attttcagga cttgttttta
    18601 tttttcgcca atatgttgtt acactatcac caatttatga atcccaatga aagtgatttg
    18661 aatgacgtgt tgatgccagc atcattaagt gatgataaaa ttatcaaaca tatggcgcgt
    18721 aagaccctca aactgtttgt aaaaaatgaa acaccgccaa aagttactca tgaagatctg
    18781 gtgaaaaaca ggcctcgctc ccctgtcaga ccgcctatac ccgcaacctc caaaacgcca
    18841 gacattcctg atcgtcatta aaccgtgatg tttaccatct ctctgacgcg tgaaagaatc
    18901 agcgtcagag aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa
    18961 ccgttttaag atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca
    19021 aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc
    19081 atcttcaaca ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt
    19141 cacatgcaaa ccgctcaggt cggcatcatg ttgaccattt acgcatgggt agtagcgcta
    19201 atgtcattgc cttttatgtt aatgaccagc caggttgaac ggcgcaaatt actgatctgc
    19261 ctgtttgtgg tgtttattgc cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt
    19321 ctggtgatca gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg
    19381 tccctggcga tccgtatggc tccggccggg aagcgagcac aggcattgag tttaattgcc
    19441 acaggtacag cactggcaat ggtattagga ttacctctcg ggcgcattgt ggggcagtat
    19501 ttcggttggc gaatgacctt cttcgcgatt ggtatcgggg cgcttattac ccttttgtgc
    19561 ctgattaagt tacttccctt actgcccagt gagcattccg gttcattgaa aagcctcccg
    19621 ctattattcc gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc
    19681 gcccattaca cggcatacag ctatattgag ccttttgtgc aaaacattgc gggattcagc
    19741 gccaactttg ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgtg
    19801 attttcggta aactgggtaa tcaatatgcg tctgcgttgg taagtacggc gattgcgctg
    19861 ttgctggtgt gcctggcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg
    19921 ctgagtattt tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg
    19981 ctggcgctgg caccagatgc caccgacgtc gcgatggcgc tattctccgg gatatttaat
    20041 attggaatcg gggcgggtgc gttggtaggt aatcaggtga gtctgcactg gtcaatgtcg
    20101 atgattggtt atgtgggcac ggtgcctgct tttgccgcgt taatatggtc aatcattata
    20161 tttcgccgct ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg
    20221 ccttttttaa tgattcgttt taatgatttc caggatgccg ttaataataa actgtacacc
    20281 catacatacc agcaggaacc ccatcaagcg ggagatcgct tcaatgccac ccttgcccac
    20341 cagccgcata attgcgccag agctgcgtag acttccccac aaaataaccg ccaccaggaa
    20401 aaagatcagc ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac
    20461 tgtggacgcc gagctaataa tcatcgcaat ggttcccgga ccggcggtac ttggcattgc
    20521 cagtggtaca aaagcaatat tggcgctggg ttcatcttcc agctcttccg acttgctttt
    20581 cgcctccggt gaatcaatcg ctttctgttg cggaaaaagc atccgaaaac cgataaacgc
    20641 gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac
    20701 cagttgcccg gcgtaatacg ccaccatcat gatggcaaag acgtacaccg aggccatcaa
    20761 cgactgacga ttacgttcgg caccgttcat gttgcctgcc aggccaagaa ataacgcgac
    20821 agttgttaat gggttagcta acggcagtaa caccaccagc cccaggccaa ttgctttaaa
    20881 caaatctaac attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg
    20941 ggtaaacaag gataaagtgt cactctttag ctaaccttgc atcgcattga ccaaaacttg
    21001 aaccgattta gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctggg
    21061 caatattatc ccctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga
    21121 tctgttcaat gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga
    21181 tcgtctgctt aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct
    21241 ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt
    21301 cgacctggga gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct gggtagaaag
    21361 gttgccgaac ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc
    21421 aatatgtgaa caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa
    21481 cctgacggcg gacgaagtgg caacacttga gcatttgctt aagaaagtcc tgccgtaaac
    21541 aaaaaagagg tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt
    21601 tggactggat cgaggacaac ctggaatcac cactgtcact ggagaaagtg tcagagcgtt
    21661 cgggttactc taaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag
    21721 gccaatacat ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg
    21781 agccgatact ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa
    21841 ccttcaaaaa ttactttgat gttccgccgc ataaataccg gatgaccaat atgcagggcg
    21901 aatcgcgctt tttacatcca ttaaaccatt acaacagcta gttgaaaacg tgacaacgtc
    21961 actgaggcaa ttatgaaacc gcttttatcc gcaatagcag ctgcgcttat tctcttttcc
    22021 gcgcagggcg ttgcggaaca aacccagcaa ccgctcgtta cttcctgtgg cgatgtggtg
    22081 gttgttcccc catcgcagga acaaccaccg ttcgatttaa atcacatggg tacaggcagt
    22141 gacaaatcgg atgcgctggg cgtgccctat tataaccaac aagccatgta gtatattcag
    22201 gccccggcat atcggggctt gttaacctct taccttcacc gctttacgcc actgcaaacc
    22261 aaatacattg atatacagtc cggccataat gagcacagcc cctaagaatt gcagacctgt
    22321 taagcgttca tccagcagta acgctgcact tgccagaccg actaccggca ccagtaatga
    22381 taatggtgcg acacgccatg tttcatagcg tccaagcagc gtaccccaaa tcccgtagcc
    22441 aactatcgtc gctacaaatg ccagatacat caacgccaga atggtggtca tgtcgatagt
    22501 gaccagactg tgaatcattt ttccggagcc atcgagaatg agcgaggcga caaaaaacgg
    22561 aattatcggg attaatgcgc tccagacgac cagagacatt accgccggac gggatgaatg
    22621 agacatgatc tttttgttga agatattgcc actcgcccaa ctaaatgccg ctgccagagt
    22681 caacataaag ccgagcatcg ccacatgctg accgttaaag ctatcttcga ttaacaccag
    22741 tacgccagaa atcgctaacg caatccccgc cagttgctta ccgtgcagcc gttcgccaaa
    22801 agtaaacgcg ccaagcacga tagtaaaaaa cgcctgagcc tgtaagacca gcgaggccag
    22861 tccagccggc ataccgaagt taatggcgca gaagagaaag gcaaactgtg caaaactgat
    22921 ggttaaacca taccccagca gtaaattcag cggtactttt ggtcgtgcca cgaaaaagat
    22981 tgccgggaag gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
    23041 aagccccact ttgatgacca caaaattaag cccccataca acgacgacta gtagcgccaa
    23101 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
    23161 ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta
    23221 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta catctctaaa
    23281 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
    23341 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgcc acgctgccat
    23401 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
    23461 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
    23521 agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc ggttttattg
    23581 ccattccttt agtgaataac gtgacgctgg tggtgctctt ttttgccctc attaactgcg
    23641 cctattccgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcatccacca
    23701 gcaaaacgaa gatcttctcg atcaactaca ccatgctcaa tattggctgg accgtcggcc
    23761 cgccactcgg cacgctgttg gtcatgcaga gcatcaatct gcccttttgg ctggccgcta
    23821 tctgctctgc gtttcccatg tttttcattc aaatttgggt aaagcgcagc gagaaaatca
    23881 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaggcactgt
    23941 tgtggtttac ctgctctggc tttctggctt cttttgttag tggcgcattt gcttcatgca
    24001 tttcacaata tgtgatggtg attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg
    24061 ttcttccagt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc cgccgcctta
    24121 acccggctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcggtctga
    24181 tcggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct gcggtattta
    24241 ctgtcggtga aataatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
    24301 caggaatgaa agccagctat ttttccgccc agtcattagg ctggttgggc gcagcggtaa
    24361 atccattagt gagtggtgtt gtacttacca ctctcccgtc gtcttcgctg tttatcattc
    24421 tggcgttagt catcattgcc gcgtgggcgc tgatgttaaa aggtattaac gccagacccc
    24481 agaggcaacc agcactctgt tgacgttcgt gccgttccac tactggcacg gcttcgctaa
    24541 tcattattct gaggcaaagg catagccttc atcttcccag ccggttatcc cgccgagcat
    24601 gattttaacc ggctttccga gtcgagccag tttgagtgcc gcacggtctg caccgttaca
    24661 atgtggcccc gcgcaataca ccacaaatag cgtatcgtcg ggccattcgg ccattctttc
    24721 tgcggtcatt tgcttatggg gtaaatggag agcgcctggg atatgccgac gcgcgaagtc
    24781 ctcttcacgt ccaaccacat gaagtaaaac gaaatcatcg ccaatataag ccagagagtc
    24841 atgaacatcg gcgcaatctg tttcgacact caagcgcgcg agaaaatgcg cctcagcaac
    24901 ttctggcttc gctgtggtaa attcagtgac ataacgcata ttgctttgtc cttttggttt
    24961 gttgtgttaa caataatgta actaatctac ctttcactga ccgcctctcg atatgccaga
    25021 aatgagcaaa atgatgacaa cgttacaacg tccgctggtc attgcactgg catatgacgg
    25081 gctatgtact tttgaatttg gcgttgccgt tgaaatattc ggcctgccac gaccggaact
    25141 gggcgataac tggtatcgtt ttgctgttgc tgcggtagaa ccaggtgcgc tacaagccac
    25201 cggggggatc cacgtggttg ctgatggtgg actggagtta ttgcccaacg ccgatatgat
    25261 tattattcct ggctggcgcg gaatagatgc caaagtgccg gagatgctat gccagtcatt
    25321 gcgcgaggca caccagcgcg gtactcgcct ggtatcaata tgctctggcg tgtttgtcct
    25381 tgctgctgcg ggccttcttc atcagcgaaa agcaaccacc cactggcgct acactgaaca
    25441 actccagcac cgttaccctt caatcagcgt aagtgacaat gtgttatatc aggatgaagg
    25501 acaagtgatg acctccgccg gaagcgccgc ggggatcgat ctgtgtttac atatcgtgcg
    25561 caaagatttt ggtcatgaga ttgctaataa tgtcgcccgg cgactggtca ttcagccaca
    25621 tcgccagggg gatcaaccac aaaatctgac gcgcccaatg gcctcaccgc gtgaaagcca
    25681 gacgttaggt gccttattcg actttttgca gcagaatctg gcacaaacac atactgtgtc
    25741 ttccctcgcc gaacgggtaa atatgagtcc gcgcactttt ttaagacgtt ttgaggcgtt
    25801 aactggcatg acgcccgccc gctggctgct caatgagcgc ttaatacgag tacaggatta
    25861 tctgcaaaat tcggttttta acatcgagaa aattgccgag ctgacgggtt ttggcaattc
    25921 agcgttattg cgtcatcact ttcaccaacg ttttttatta tctccttcgc aatatcgcaa
    25981 aaacagccgt tccccatcac aataatcgcc tgattcagcc tttgtttaga taacctctgc
    26041 taatatcttt ctcattgaga taaaaattaa ggtaagcgag gaaacacacc acaccataaa
    26101 cggaggcaaa taatgctggg taatatgaat gtttttatgg ccatactggg aataatttta
    26161 ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata
    26221 atccctcacc taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg
    26281 gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
    26341 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
    26401 ttttctcaat gaatgttaaa cggagcttaa actcggttaa tcacattttg ttcgtcaata
    26461 aacatgcagc gatttcttcc ggtttgctta ccctcataca ttgcccggtc tgctcttcca
    26521 atgaccacat ccagagtctc ttcaggaaat gcgcgactca cacctgctgt cacggtaatg
    26581 ttgatatgcc cttcagaatg tgtgatggca tggttatcga ctaactggca aattctgaca
    26641 cctgcacgac atgcttcttc atcattagcc gctttgacaa taatgataaa ttcttcgccc
    26701 ccgtagcgat aaaccgtttc gtaatcacgc gtccaactgg ctaagtaagt tgccagggtg
    26761 cgtaatacta catcgccgat taaatgcccg taggtatcat taaccaattt aaatcggtca
    26821 atatccaaca acattaaata aagattcaga ggctcagtgt tgcgtaactg atgatcaaag
    26881 gattcatcaa gaacccgacg ccccggcaat cccgtcaaaa catccatatt gctacggatc
    26941 gtcagcaaat aaattttgta atcggttaat gccgcagtaa aagaaagcac cccctcctga
    27001 aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa tagccagcat taattcccga
    27061 ccacaattat gcatatgttg atgggcagaa tccattagcc gaacgtaagg taattcatcg
    27121 ttatcgagtg gcctcagatg atcaatccac cgaccaaact ggcacagtcc ataagaatgg
    27181 ttatccgtta tttctggctt actggcatct ctcgcgacca cgctgtgaaa catactcacc
    27241 agccactgat agtgggcatc gatagcctta ttgagattta acaagatggc atcaatttcc
    27301 gttgtcttct tgatcattgc cactcctttt tcacagttcc ttgtgcgcgc tattctaacg
    27361 agagaaaagc aaaattacgt caatattttc atagaaatcc gaagttatga ttcatctctg
    27421 agataacatt gtgatttaaa acaaaatcag
//
LOCUS       DAZFKY010000050        27399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFKY010000050 DAZFKY010000000
VERSION     DAZFKY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            124..1140
                     /locus_tag="ACHKCL_001868"
     CDS             124..1140
                     /locus_tag="ACHKCL_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5762847.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1225..1647
                     /locus_tag="ACHKCL_001869"
     CDS             1225..1647
                     /locus_tag="ACHKCL_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5762848.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1644..1847
                     /locus_tag="ACHKCL_001870"
     CDS             1644..1847
                     /locus_tag="ACHKCL_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5762849.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1851..2258)
                     /gene="cueR"
                     /locus_tag="ACHKCL_001871"
     CDS             complement(1851..2258)
                     /gene="cueR"
                     /locus_tag="ACHKCL_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5762850.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2255..3430)
                     /locus_tag="ACHKCL_001872"
     CDS             complement(2255..3430)
                     /locus_tag="ACHKCL_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO5762851.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3427..5589)
                     /locus_tag="ACHKCL_001873"
     CDS             complement(3427..5589)
                     /locus_tag="ACHKCL_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO5762852.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5593..21330)
                     /locus_tag="ACHKCL_001874"
     CDS             complement(5593..21330)
                     /locus_tag="ACHKCL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5762853.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASLAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVT
                     VSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDAS
                     GNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDD
                     IINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALG
                     NGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGS
                     SSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGT
                     QTSITHPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGG
                     KTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPT
                     VTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTL
                     PASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEH
                     IQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTIS
                     ATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATG
                     TVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASR
                     SVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATV
                     AANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVA
                     GDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAG
                     LSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVIS
                     GTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAI
                     GNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNE
                     TAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVI
                     DTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGG
                     VTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAA
                     DSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGCPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(21367..25752)
                     /locus_tag="ACHKCL_001875"
     CDS             complement(21367..25752)
                     /locus_tag="ACHKCL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5762854.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25855..27210)
                     /locus_tag="ACHKCL_001876"
     CDS             complement(25855..27210)
                     /locus_tag="ACHKCL_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO5762855.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5129 a         8048 c         7920 g         6302 t
ORIGIN      
        1 tttacatcac acaaatggcg gcgtagattt cgattaaatt gcaacgcagt ttatttctta
       61 aaacaatatt atttgtttct tatagaaaca ttaatacgac ttattttgaa caagagaaaa
      121 tgaatgaaaa ctgtaaacgt agctttactg gcactcataa tttcagcaac atccagccct
      181 gttgttttag ctggtgatac cattgaagcg gcggcaacag agctttcagc cattaactct
      241 ggcatgtcgc aatcggagat tgagcagaag attacccgct ttttagaacg cacagacaac
      301 agccccgctg cgtataccta tttgactgaa catcactaca tcccttctga aacacctgat
      361 accactcaga ctcccactgt ccagacagat cctgacgcag gacaaaaaac cgttgccgct
      421 acaggtgatg tacagacaac tgcccgttat cagagcatga tcaacgcccg acagtctgcg
      481 gtaactgacg cccagcaaac gcaaattaca gagcaacagg cgcagatcgt agccacacaa
      541 aaaacgctcg ccgcgactgg agatacgcaa aataccgcgc attatcagga aatgattaat
      601 gccagactgg cggctcaaaa tgaggctaat cagcgcaccg ccactgaaca agggcagaaa
      661 atgaatgcgc tgacaaccga tgtggcagta caacagcaaa atgaaaggac tcaatacgat
      721 aaacaaatgc aaagtctggc gcaggagtct gcccaggcac atgaacaaat tgacagcctg
      781 tcacaagacg taacccaaac gcaccaacag ttaaccaaca cccaaaaacg ggttgcagat
      841 aacagccagc aaattaacac gctcaataac catttcagtt cgctaaaaaa cgaagttgat
      901 gacaatcgta aagaagccaa tgcgggaact gcatctgcca tcgctatcgc ctcacaacca
      961 caggttaaaa ccggtgacgt gatgatggtg tcagcgggag cgggaacctt caacggtgaa
     1021 tctgcggtgt ctgtcggaac atcatttaat gccggaacgc atacggtact taaagccggt
     1081 atttctgcgg atacacaatc tgatttcggc gcaggtgtcg gcgtgggata ttcgttctaa
     1141 tatttcaatc ctcaatataa ataagagcaa ggaagcttgc cgggttcacc tcttcattaa
     1201 tttgtacatt atttaaggtt aacaatgatg aatagctcca ttaaatcgtt ttccctgctg
     1261 gcggttatat tactggctgg ctgtagttca cccacttccc gcatcgcaga ttgccaggcg
     1321 cagggcgtca gtcatgacac ctgttacctc gcagaacagc agcgtcaggc ggctatttta
     1381 agtgcatccg aggcacaggc atttaaaaat gcagaagccg cacaacacgc ccaggcggca
     1441 aagaaagcca tttataaagg atttggcatg acctttagaa tgagcagtaa aaactttgct
     1501 tatctcaatg attcattatg tgcaattgat gaagacaata aagatgccac tgtttatcag
     1561 tcaggtctat ataacgtcat tgtttatcat cacacaggaa aagtcgcctt aatgaaagaa
     1621 ggccagtttg tgggttattt aaaatgaagg agcaaaggaa aatacccctg acgcatatta
     1681 tgattatcgg tgcgtttatt tttgccttct tgcaagtagt attattagcc tccctggttc
     1741 acgctgtgaa tgttaacaac gaaatccagg aaggcttatt tcagtcgggg cgcattatgg
     1801 tagaaagttt gcagcatatt ctttcggtgc aaacggggat tcactgattt tcaccccgcc
     1861 cgatgatgac agcagccgga gagattttcg ataatcgggc agtcggcgct gtcatcaccg
     1921 gggcaggcat tcgccagcgc cagtagctgg ttgcgcatag attgcagttc ttcgatatgc
     1981 cgttcaatct ccgccacctt ctccagcgtg cgacgtttga cgtcggcact gtgacgctgc
     2041 gggtcgttaa acagattcac cagctcgccg ctctcttcca ggttaaagcc cacctggcgc
     2101 gcctggcgca gtaaggtcaa ttcgttgaga tgctgctgcg tgtaggttcg ataaccattt
     2161 tcgctgcgca tcggcggcgt caccagcccc ttctcttcat agaagcgaat ggctttgctg
     2221 gtcaggccgg taatttttgc tacatcgcta atgttcatcg ttcgcgcaac gcctcctgtg
     2281 ctttgttaaa gggtttcaac agataatcca gcacgctttt tttgccggtg cggatatcga
     2341 ccgtcgccac cataccgggg aatatcggaa actgctgttt gcttttgttt tccagatggt
     2401 tgctgaaagt gcgaatgtac acccgatagt aataaacatc gcgccgcact tcatcctgca
     2461 cggtgtctgg tgatatcacc gccacttctc ccggcaggcc gccgtagatg gagtaatcgt
     2521 aggcagttat tttcaccagc gatttttgcc ccgggtggat aaacgccaca tcgcgcgggg
     2581 agatttttgc ctcaatcagc agttgctcat ccagcggcac gatggtcatc agtttaccac
     2641 cgggggcgat gacgccgcca accgtggtga catcaatatc ctgaacaatg cctcgcaccg
     2701 gcgcggtaaa gttgagcctg gtgagagagt cttcccgtcc acggatcacc gaacgctggg
     2761 tttccacttc cgcattggct tttgctaact cttcgcgcgc ctgaacgtag tattgcgtgc
     2821 gcacgtcgct gagtttgttt tccagttcat tggcctggcg ttgcaggcgc agtacttcga
     2881 cgctgctcgc tgccccctgt tttaccagcg gctgggtcat cgccagttcg cgattgacca
     2941 ggctaatccc ctgcgtcagc ccggtggtgg ctttgttcaa cccgtcgcgg cgagtgttgt
     3001 acaacgccgt ttccgactcg ataagttcgg ggaatttatt cagttcgtca gggaagacca
     3061 gcggtttatt gctcacttcc gcccgcagcc gcgccgaggt tgccagcgcc gcgtacagcc
     3121 gcgacatcgc ttcctggacg tcggattccg ttttggtgcg gttaagctgc gccagcggct
     3181 ggccctgttc gacaatatcg ccaactttca cgtcgagatg atacagaatt ccgccttcca
     3241 gcgactgcac cacctgttcg cgcccggaag gttctaccgt gccgctgccg gtggtgactt
     3301 catcaagctg gaagtaccac gcccagccga gaaaacagag cagcatcaca gcggtaacgc
     3361 gtaccgccag cgccgaacgc ggcaggcggg gttcagtcag accacggtta tattgcagtt
     3421 gtccgttcat gccgcagacc ccttattctt ctcctgcaac accacccgtc gcgctgtggg
     3481 ctcgccctgt tcacgcaaaa tggcctcttt cgcgccgtcc atcacaatcc gcccctgatc
     3541 catcacgatt atcctatcca ccagttgcag catcgccgtg cggtgagtgg cgataatcag
     3601 cgtgcgatgc ccaatccact gtttcagttg ttcgattaaa tacgcttctg ccatttcgtc
     3661 cagtgaggcg ctgggttcgt cgagcaggac gatattcggc tgagagatga gtagccgcgc
     3721 cagcagcagc gtttgccgct gaccaccaga gaggccaaat cccccttcct gaatcatgta
     3781 gttgaggccg tttttctgct tctgcacaaa gggcagcgct ccgctgaggg ttaacgcgcg
     3841 ttcgatatct tcatcgctgg cttccggcat ccccatggtg aggttttccc gcagcgaacc
     3901 aaagaacagt cgcccggtct ggctgagtag ccccatgtct cgccgcagat ctgccggatc
     3961 aagctgcccg atactgatgt tatccagcaa cacctgcccc tgctgggcga tacgcattcc
     4021 ggcgagcatt tgcagtagcg tcgatttacc cgcgccgttg cgcccgagaa tggcgatttt
     4081 ctcaccagcc tgaatgttga gtttgccaat cgctacatcg gcaatcttct cttcttcgtc
     4141 gtaataaaac accgcgttgc tgaactgata attaccgtgc agcaccgctt tatgcaccag
     4201 tttgccgtgt tccggctgat ccaccgggcg cttcatcagt tcatcaaggc cgttacgtgc
     4261 tactttcgcc tgctgccagc gcgagagcac gccggatatc tgcgacagcg gcgcaatggt
     4321 gcgcgaagcc aggatcgtcg tcccgaccag cgcgccggtg gtcatgtcgc cgttcatcac
     4381 cataaagcag cccaccagca gcaccaccac gtagacgatg gactgcactt cctgggtcca
     4441 ggtgagcagc agcccggtga gaaaacgttg cttcatgctg atggacgagg ccacgtcgtt
     4501 ggtgtggttc cactgattct gaaaacgctg ttcggcgcgc agcagtttaa tatcttcaat
     4561 cgactgcacg gcttccacca gcgtagcgtt gcgtaccgct gactcgcgca ttccttcgtt
     4621 cgccagccgc gccagcgggc gttgcaccag caggccgggg atcaccagca acggcagcgc
     4681 cagcaaaacc accagcacca gccagccgcc gatcatccac aaaataaaga caaacagcag
     4741 gaagaatggc aggtccgcca ccgcgccgat ggtggtggac gtaataagct cccgcactga
     4801 ttccagttcg cggatctgcg agataaacga tccggtggat ttcgatcgca cgttgttttt
     4861 cagccgtagc gcatgaccaa aaacgcgatc cgaaatacgc aggtctgccc gctttccgat
     4921 cacatcagat agatgcgtac gcaccatgcg catacagaac tcaaacagga tcgccatcat
     4981 caccccggcg aaaagcaccc atagcgtcgg gtacgattgc gccggaacca cccgatcgta
     5041 aacctgcatc gagaaaatca tcgccgccag cgccagcacg ttcgccacca gtgacgccag
     5101 cataatatcg ccgtagcgcc gccagtcttt tagcgaaaga ctccagaacc agttcgcctg
     5161 atacgggcga atatagtcat ccacccgcgc gtcagggatg gcgctgagag ggcgcagcag
     5221 cgccagttcg acgattttat cttcaatatc cgcgacgtta agtgtagttt ccagcccttc
     5281 gtcaccgctg aactgaacgc tgatattgtc atgattgtcg cgacgggtga tcacgccaat
     5341 ctgctgatta tcaaacaccg cgatcagcgg cagacgccag gggttaagcg aatcggcgga
     5401 aaactccacc aaccgcaacc ccatccctaa ctgccgcgcc atgtcggtga gtaactcctc
     5461 ccgcttactg tcgcgctccc agttgacggt cgcgcgcaca ttctcctgag aaaaatcgag
     5521 ccgataatag cgcgccacgg cgatcatcgc ctccagccag gcttccacgg actgggcatt
     5581 ttttttcatt atctacacca ccatcaactg atgattcgcc agcaaggtgg cgaggtcggt
     5641 gtgcacgccc gtcagcgtga ccacgttagt tgtcgcaaaa gtgccgcccg tgccgtcgcg
     5701 gtcgatctgc acgatggtgt cgctgccgct ctgggtgact ttgacaaagt caccgatgtt
     5761 tccggcctgc gcatccagcg ttgccacgcc gttgacgtag ctggctttgc cgttgccggt
     5821 gtagccgctg ccctgtaaca gttcacgaat atcaatgcga tcggtgtcgg cggtgccttc
     5881 ccatgtccct accgtaaagc cattcaccac gtctgagccg ttgccgccag tggcgtcgct
     5941 ggcgttgagc agtttataga gcaaggtgtc gtggccgccg ttgccgatgt tgagcgtgtc
     6001 gttgccgccg cgcccttcca gttggttatc gccgctgctg ccggtcagaa tgtcgttaaa
     6061 attcgagccg gaaatgcctt cgatattggt aaatgtcgag gtgttaaaac cggtggcctg
     6121 cgcggcagtg ctgcttaagt tagccgtcac gcccaccgtc gaaagccgga aatcaaccac
     6181 gtccatcccg ccgttcgcca gccacgttcc ggtgccggag ctgtacaccc agccgccgga
     6241 accgtcgtaa gtgtcggtgc ctgcggtggc gaagaaggtg tcgttatagc cggtgccgac
     6301 gaacttgccg ttgttgctcg cggtacccgc ttcagcgctg agatcgtaag cgtgagtggc
     6361 atcggtggcc tgtaaaccgc cccagctggt gttgaccgtc tggtcgatat tgctggcggt
     6421 ggtgccggtg gatttgatca gcaccgcgtt gtaggtttct ccggcattca gcccgtagaa
     6481 gttgaccagc gccgaggtgt cgttaacccc catacccgac tgcgggttga tgatttgcgt
     6541 ggcgaccagc actcccgccg agttgtacag cttcacggta ttgccgtaat agacgttaat
     6601 gccgttggga tcggtgatgc gcaggtgtag cgaagtgccg tagctcaccg tgttggtgtt
     6661 gcggctcagg aacacgctgc ccgactgttt ggacaccagc acatccaccg cgccgtccca
     6721 gtcgtagtcc attgccgcca cgccgctggt ggtaccgctc tggctgccgc cgagggccga
     6781 ttgcgtccag ttgctggcgt tgctgacgtt agtataaaga tacgactgcc cggtctgggc
     6841 gatacgggcg atatccatca ggccgtcgtg gttccagtcc accgccaggc taaactggct
     6901 ggcgtaggtg gttgccgttg cgcccaccgc caccgggcaa cccagtacgc cgttggtgtt
     6961 gaataacagc gagccgtagt tagctcttcc ctggctggcg ggcaggaaga gatccatatc
     7021 gccgtcacca tcgaaatcgg cccaggtcat ggaaacgctg ctgctcatag cgccactgcc
     7081 cgccccgctc aggaaggtat tggtggtgtt ctgcccccag acaaacgtcc cattcccctg
     7141 gttgatgagc gaagacagcg tgtaatagtt gttcaggtta taggtgtgct gaaccaggtc
     7201 gatcctgccg tcattgttca gatcgacgcc agaaccttcg ttgagcgaaa gataccccgt
     7261 caccgccccg cccaccgtgg cgctaccgcc gctgttcgac gtggtggagt tgcctaccag
     7321 cgtgcctgcg ttgttccaca ggaaggtgtt ggagtccggc ccgccagcgt caccaatcca
     7381 gaagtcgaga tagccgtcgc cctctttatc aaatgccaca atcgagccgt accacgtcag
     7441 ggtgccgacg gtgacctgaa tagcgctgga aaaggtgcca tcgccgttat tggtaaagcc
     7501 gttaatatag cccgtaccgt agtcatcacg ggtgataaac agatcgcccg tgccgttacg
     7561 gtcgtaatcg gcaatggagc tggtggcgta gttgttgccg ctctgataca gcgtcagcgc
     7621 ggtcttcgac caggtgagtg ggtcagtcga ttgcattacc tgctggtttg ccagcaccgt
     7681 ccacatcccg tgcgagtcga ggccgtaggt cagcccccag gcggtggttt tgctggcggt
     7741 agtccaggtc ggtgtgtaat caatcgccgc gttaaccgtc accgtgccgt tgctaatatt
     7801 ggcgttattg ccgttacccg ccgaactttt tacctgcgca gtaacggtat aggcggtcgc
     7861 ggaaactgtc agcgcatcgg tatccggcaa ctgtacatac caggtgttgt gcgccggatc
     7921 gaccactacc gcgccgcccg gttcagaggt gtaggttttg ccgttgatca ccacttcaac
     7981 atactgccca ctggcaagcg cggcggtgat caccccgcta ataatcggcg tagtatcgcg
     8041 cgtggtctgg ctggtgattt gcgccagcgt ggtcggaata gaggtatcga ccgtcaggac
     8101 aaaatcactg gaggaggtga tattccccgc caaatccacc actcgcgcgc tgtaggtata
     8161 ggcaccgctc accagcccgc tgtcgctgta ggtccagttc agagcgccga ccatcgtcac
     8221 cgcccctaac agcagcccgt tacggtagag gtaaaccact tcaccgctgg caagcggcgc
     8281 ggaaagtaca ccgttcagca gcggcgtagt gtcgtcggtg gcctgcgaac tgcttaatgt
     8341 cccctgccgc tgcccgacat catcggtata cgacacaatc gttggtgtgg cggtggggtt
     8401 aatcgtatcg accaccaccg ttttcgacac caccgggccg gtgttccccg ccagatccag
     8461 cacccgcacc tgataaacgt agctgccgtc ggtcagcgtg cggctgtcgg tgtaagtcca
     8521 ctgtgtaccg ttggtggtga gcgttgtcca ggtcgcgccg ctatcaaggc taatctgcgc
     8581 cacttcgccg ctggcaagcc ccgcccccag cgaaccggtc agcgtgagca tggtgtcgct
     8641 ggtgataaag tcagttgccg aactccccgt gtcggtgctg atgctggcga tggtgagcac
     8701 ctgcgccggg ttaacggtat cgatcagcgc cgactggctg ctcgttgcgc caacgttgcc
     8761 cgccagatcg accacccgca cctgccaggt ggtggtgccg ttggtgaggg ttcgtccgtc
     8821 aacgtaactc cagttcaggc tgtctgcggc gacggtcacg ttcacccagg ttgcgccgtt
     8881 gtcggtactg atttgcgcga actcattagc gccgagcgcc gcgcccaata cgccgcgcac
     8941 ggtaagcgtg gtgtcgctgg taataaaatc gctgctgctg gttccggtgt catcgctgat
     9001 accggtaatg gtgatacttt tcgccgcttc cgggctggtg gtgtcgacca ccacgttttg
     9061 cgttgcggtc tgcccaacgt tccccgccag atccaccacc cgcaccgtgt agttccaggt
     9121 gccgtcagta agcgtgtgac catcggcata gctccagctg gtgccaacga cggttaacgt
     9181 agtccaggtg acgccgccgt cgaggctgat ttgcgcgaac tcaccggcag aaagcgtcgc
     9241 ccccagcgtg ccgctcaccg caagcgtcgt gtcgctggtg acaaaatcgt tagtgatcaa
     9301 gcccatatcg gtggtgatcg cgctgatggc gatggttttc accgcgggat ctggcgcggt
     9361 agtgtcgatc accacattct ggctgtcggt cgcgccaacg ttgcccgctg cgtcaatcac
     9421 ccgcacctga tagagatagt tgccatcggt cagcgtgcgg ctgtcgttat agcgccaggt
     9481 cgtgccggta acggtaagcg tggtccaggt tgtgccgccg tcgatactta tttgcgccgt
     9541 ctcgttactg gcaagcgcgg cggtgagcga gccgttgacc actaccggac tgacgctggt
     9601 gataaagtcg ctggcgctaa ggccagtgtc ggcttgcagg gaatcgatat taatgcccat
     9661 cgccggagcg ctgagatcga cggtaatggt gtgattactg ctaccggtgt tgccaatggc
     9721 attgctcacc gaggcgttaa tcacgtaggc gttgccgtcc gccagcgcgg tgacatcggc
     9781 gctgccgagg gtgtagctcc agctaccgcc cgtctgcacc gtggtggtgt aggttttgcc
     9841 gtttaaggtc agggtcagcg tctgcccgac gggcgcggtg gtggtgccgc tgatcaccag
     9901 cgatgagccg tgttccgctg cattcaccac atcgtcgccc gcaaaggtgt tgatggtgac
     9961 agtaggtaca tcgccgttca gcgtcacgcc acggctggcg ctgccggggt ttccggcctg
    10021 atcgctaacc gtcgcgctaa tggtgtagct gccgtcgctt aatccggcaa actgctgcgc
    10081 cggaataaac accgaccagc ttccgccgct gccaaccgtt gcgctccagg tttgcccgtt
    10141 aaaggtaacg gtcaccgtct ggcccacttc ggcggtggtg gtgccgctaa tggtctgccc
    10201 ggcaagctgc tcggcgttgt tgataatatc gtcacccgcc accgtggcga tggtgaccgt
    10261 tggcgcaacg gtatcgacgg tcagggcgtg cgtggcctgt ccggggttac cggcgcgatc
    10321 gttcacgctg gcggtaatgg tctgcgcgcc ctgcccgagc gcggccagat ccaccgcagg
    10381 cacgttcagc gcccaggtac cattggcggc aaccgtggcg gtataggttt tgccgccaaa
    10441 ggtgacggtg accgtctgcc ccacttccgc gctggtcgag ccgttaaggg ttaacggctg
    10501 ctgctgttcg gcggcgttga ggcggtcgtc cgtcgaaacg gtgttaatgc tgataaccgg
    10561 cgcggtgaga tccaccgcca cgctgcgcga ggccgtggcg ctgtttccgg cactgtcctg
    10621 agcggaggcg ctaaccgtgt agctggtgcc atcactgagt gccgcaacat ccgccgcagg
    10681 cacgttcacg ctccagctcc cgttgctctg aatggtggcg ctgtagcctt tgccgttaag
    10741 caacacggtc accactgtac ctgtcgcgaa attagtgcca gtgccgctca gggtcagcgc
    10801 actgcccgct tcggcggcgt taataaggtt atcgccgctg atagtgctga ccgagagcga
    10861 cactgccgcc gtgttcacct gtacgttgtg cgtctgcgtg ctgctgttgc ctgcgctatc
    10921 ggtaatggtg gcgctgatgg tcaccgtgcc atccgcaagg ccggaaatca cgctggcagg
    10981 cacgccgacg ctccagttgc cgctggcatc ggtgctggtg acatactgct gcccggcgat
    11041 ggtcaccacc aggcggtcgc ccgccaccgc gcccgtggcg gtgccgctga taatctgcgc
    11101 ctgaatgtgt tcgacgttgt taatcacatc atcgcccgcc acggtgttaa aactgatcac
    11161 cggcgcagtg gtatcgacgg tcacgccttt gctggcgctg ccaaggttac ccgccagatc
    11221 gctgaccgtg gcggtcaggg tgtagccgtt attagcaagt gcggttaagt cgctggcggg
    11281 cagcgttaaa ctccagctgc cgtctgtctg caccgtggtc tggtagttag tgccgttaag
    11341 cgtgacggtg agcgtctgcc cggtttgcgc ggtgctggtg ccggacaccg ttacgcccgc
    11401 cgccgcttcg ctggcgttga tgatgttatc gctggcaatg gtgttgatgg tcaccgttgg
    11461 cgcggcgcta tcgacgctgt attcgtgcgt tgctgtggcg ctgttgccgt tgacgttagt
    11521 cacccgcacc tgggcgctgg catcgccatc acgcagggcc gccaaatccg ccgccggaac
    11581 ggtggtgctc cagctaccgt ttgccgccac cgtggtggtg taggttttgc cgccaaaggt
    11641 gacggtgacg gtttgccccg cttcgacgcc ggacgtgctg ccggagagcg ttaacgccgc
    11701 gcctttttcg gcggcgttaa tcgcatcatc gctggtgatg ctgttcatgc tgatggcgac
    11761 ggcggtgaga tcaacagtca gcggatgggt aatgctggtt tgcgttccgg cactgttcgc
    11821 cccgctaacg gtaatattca gcgttcccgc aggccagtta ctgacatccg tcgcaggaac
    11881 gccgacgctc cagctaccgt tcgccaacac cgtggcggcg taagtttggt tgttaatcac
    11941 caccgtcaac gccgtgcctg cggcaaagcc ggagctgctg ccggtgatca cctgcgcctg
    12001 agcgtgttcg atgatattga ccacatcatc acccgcgacg gtatccaccc gcaggccagg
    12061 cagactggca tcgatggtga tatcgcgcgt gccgctaccg gtgttgccaa cggcgttggt
    12121 caccgaggca ttaaccgtta actcaccgtt acccagagcc tgccagtcgg cggcaggtac
    12181 gctcacattc cagcttaaat cagcctggac ggtggcggtg taagttttac cgcccagttc
    12241 aacggtgacg gtgctgcccg ccgccgcacc ggttacctgg ccgctgatag tttgcgcgct
    12301 gcctgcctct gaagcgttga taatatcgtc cgtcgccacc ggattaatgg tgatgcccgg
    12361 cagcgcggta ttaacctgca cgttatggct ggcggtgctg ctgttaccct gagcattggt
    12421 gacgctcgcc gtcacgctgt aggtcgcttc gcccagcgca ctgaccgccg aggcaggcac
    12481 ggtaacactc cagttgccgg aggcatccgt ggtggcgctg tagttttgtc cgttcagggt
    12541 gacggtgatc gccgtacccg caggctgatc gctggtgcca gaaagcgcca gatcagatcc
    12601 tttttccgtc gcgttgatca catcatcgcc cgcgatggtg ttgatgctaa tcactggcgc
    12661 gctgaggctg acggtcacgg tgcggctggc gtcgtcgctg ttgcctgccc gatcgctgac
    12721 gctggcggtg atggtctgcg caccgctacc cagcgccgta acatctgccg ccggaacgcc
    12781 aacgctccag ttgccggagg catccagggt ggtggtgtag gttttgccgt tgagcacaac
    12841 gctcaccaca tcgcccgctt ccccgccgct actggtgccg gagatcacca gcgcctgcgc
    12901 atgttcggca tcgttgatga tgtcatcgcc cgccacggtg ttaatggtga gaaccggcgc
    12961 ggtggtgtct accgccagat tatggctggc gctgccgggg ttgcccgctt tgtcattgac
    13021 cgtggcgctg acggtgtaat tgcttgcgtt gagcgcgccc agggctgaag tgggaacgct
    13081 gacgctccag ctaccgtccg cctgcacagt gccggtgtag gttgcgccgt taagggtgac
    13141 ggtcaccgtc tgcccggttt cggcggtgct ggtgccgctg atggtgagcg ggcttccggc
    13201 ttctgcggcg ttaaggatat cgtcgctggc gatggtgtta atggtgagcg tcggcgcgct
    13261 ggcgtcaacg ctatacgcat gggtcgccga ggcactgtta ccgctcgcgc tgctaacgct
    13321 ggcctgcaca ttcgccgcgc cctctggcag agttgccaga tctgccgccg gaacgtttac
    13381 actccagctc ccgttggcgg cgaggcttgc agtgtaggtt ttaccgccaa aagtgacggt
    13441 gacggtttgc ccgctctcaa tcccgctggt actgccggaa agagtcagat tggtgccttt
    13501 ttctgcggcg ttaatcacgt cgtcgctggc aacggtgttg atgctgattg ccaccgccgt
    13561 taaatcgacg gtgaacggat ggctgacgct gactgggtta ttggcgctgc tttgcccgtc
    13621 tacctccacg gttaacggcc ccgcaggcca ggcgctgacg tttgccgccg gaatgccaac
    13681 gctccagctt ccgtccgcct gcaccgtggc ggaatacgcc acactgttga tggtaaccgt
    13741 cagcacagca cctgcattca ggccgctact gccgccggtg atcaccagcg cctgcccgtg
    13801 ctcgatgctg ttgacgatat catcgcccgc cacggtatct acgcgcagcc ccggcaggtt
    13861 ggcatcaatg gtgatatccc gcgtgccgct cccggtgttg ccattagcgt tggtgaccga
    13921 ggcggtaatg gtcaaatcac cattgcccaa cgcctggaga tcggctgtcg gaacgctgac
    13981 gctccagctt aagttgctct gtaccgtagc ggtgtaagtg ttcccgccca gcgtcacggt
    14041 gaccgtatcg ccagcggcgg cgcgagtcac caccccgctg atggtttgat cggcccccgc
    14101 ttcggcggcg ttgataatat cgtcgcctgc cacggtgttg atggtgacgt ccggcaggcc
    14161 gctgtcgacc agtaaattcg cctggctgct ggcggtgttg cctgctgcac tggtggcgct
    14221 ggcgctgacc gtatagttgg cctgacctaa tgccgccaga tcgctcaccg gcacggttaa
    14281 ggtccagttg cccgatgcgt cggtggtggc gctgtagttg tgaccgttga gcgtgacggt
    14341 gacctgcgcg cccgtcgcca gccccgtact gccgccgctg atggtcaacg gctggccttt
    14401 ttcatcggcg ttcaggatgt tatcgccgct gatggcgtta aaggtaatgg tcggcagccc
    14461 ggtattgacc gtcacctgat gggtagcgct gccgctgttt cctccggcat cggtgacgct
    14521 ggcattgatg gtcaccgtgc cattcgccag tgccgagacg acgcttgccg gaacgccgac
    14581 gctccagttg ccgctggcat ccagcaccgt ggtaaaggta tttgtgccga tagtcaccgt
    14641 cacggtgcta ccggttgccg ctccagtggc ggagccgctg atgatctgcg cctgggcgtg
    14701 ttccgtcgcg ttgatcacat catcgcctgc caccgtgttg atggtgacga ccggaacgga
    14761 cgtatccacc gtcaggttgt gattaaccga ggccgggttt ccggctttgt cgctcaccgc
    14821 cgcgttcacg gtgtagttgc tggcggtcag ggtgcttaag tcggctgacg gtacgctgac
    14881 gctccagctg ccgtccgtct gtacggtgcc ggtgtagttt gtgccattga gcgtgacggt
    14941 caccgtctgc cccgcttccg ccgtgctgct gccggtgatg gtcagagccg ctccggcttc
    15001 ggcggcgtta agaatatcat cgcccgcaat ggtattaatg gtcaccgttg gcgcgctggc
    15061 atcaacgctg taagcgtggg tcgtggtggc gctgttgccg ttaacattgc tgacgctggc
    15121 ctgtgcgctg gcatcgccat cacgcagagc cgccatatct gccgccggaa ccgaggtgct
    15181 ccaggaacca ttcgcagcca ccgtggcgct gtaagttttg ccgccaaagg tgacggtaac
    15241 cgtttgtccg gcttcaacgc cagaggtgct gccggagagc gttaacgccg cgcctttttc
    15301 ggcagcgttg atcacatcat cggcggtaat ggcgttgatg ctcaccgcca ccgccgagag
    15361 atcgaccgtc accggatgcg taacgcttac cggatttccg gcagaggtgc taccgctcgc
    15421 cgcaatcgtc accgatcccg caggccaggc gctgacatcc accgccggaa cgccgacgct
    15481 ccaggtgcca tccgccagca ccgccgcaac ataggtttgc ccgttaatgg tcagcgtgac
    15541 gttgctgccc gccgccagac cggagctgct gccggtaatc accagcgcct gcccgtgctc
    15601 gataatatta accacatcat cgcccgccac ggtgtcgacc cgcagaccgg ggagattcgc
    15661 gtctatggtg atttcgcgcg tgccgttacc agtattgcct acgctgttcg tcaccgaagc
    15721 cgaaatggtc agttcgccgt tgcccaactc ctgtagcgcg gaggccggaa catcgacgct
    15781 ccagcttaaa ttcgcctgca ccgtggcagt gtaagtcgcc ccgccgagcg tgacggtcac
    15841 cgtgtcgcct gccgccgcac ccgtcacctg cccgctgatg gtctgttcca cgcccgcttc
    15901 ggcggcgtta ataatatcgt ccgttgccac cacgttaatg gtgacgcccg gcagcgcggt
    15961 attaacctgt acgttatggc tggcggaacc gctgttgcca tcggcgtcag tggcggctgc
    16021 ggtcaccgtg taggtggctt caccgagggc gctcacccgt gacgccggaa cggtcacgct
    16081 ccagttaccg gaagcatcgg cagtggtggt gtaattttgc ccgttgagcg taacggtaat
    16141 ctgcgtgccc gcaggctggt tgctggtgcc ggtaatcgcc agatccgcgc ctttctccgc
    16201 ggcgttgatg atgtcatcga cggcaatggt gttaatggcg agcaccggag cgccgagcgc
    16261 cacggtgaca gtgtgcgagg ccgtgccgct gttgcctgcg gagtcggtga ccgtagcggt
    16321 aatggtcaca tcgccctgcg ccagcgcgga aatcacgctg gcaggcacgc cgatgctcca
    16381 gttgccgttg gcgtccagca cggtggtata ggtggtcgtg ccaatcgtca cggaaaccgt
    16441 attaccggta gtcgcgcccg ttgccgagcc gctgatgatc tgcgcctgtc cgtgttccgt
    16501 ggcgttaatg atgtcatcgc ccgccaccgt gttgatggtg acgaccggag cggcaaggtc
    16561 caccgtcaga ttatgcgttg ccgaagccga atttctggct ttgtcgctga ccgaggcgtt
    16621 aacggtatac gagctggcgg taaggctagc taaatcgccg gttggtacgc tgacgctcca
    16681 gctcccgtct gcctgcacat tgccgctgta attaacgccg tttagcgtta cggtcaccgt
    16741 ctgcccggct tccgccgtgc tggtgccgct gatggtcagc gccgatcccg cttcggcggc
    16801 gttaagaata tcgtccgtgg cgatggtgtt gatggtaacg gagggcgcag tggcatcaac
    16861 gctgtaagca tgcgttgcct gggtgctgtt ccctgcgaca ttgctgacgc tggcctggac
    16921 gtttgctgcg ccgtctggca aggttgcaac atcgacggca gggatcgtca gcccccaggt
    16981 attatccgcg gcaaccgtgg tggtgtagct tttgccgccg aagataacgg tgatggtttg
    17041 ccccgcttcc acgccggagg tggtgccgga gagctgcaaa tcagagcctt tctcggcggc
    17101 gttaatcaca tcgtcagtgg aaagggtgtt gatggtgatc gccacggcag cgagatcgac
    17161 cgtgaccgga tggctaatgc tggttgtggt tccggcggtg ttggtgccag agacggcaat
    17221 attgaccgtc cccgcaggcc agtttgtgac atctgccacc ggaacaccaa cgctccagct
    17281 accatccgct aaaaccgttg cgccgtaggt gacgctattg ataaccaccg tcaacgccgc
    17341 gcccgccgcc agtccgctac tgctaccggt gatcaccaac gcctgagtgt gctcgatgct
    17401 gttaatcaca tcatcgcctg ccacggtatc gacgcgcaga cctggcaggt tggcatctat
    17461 cacgatatcg tgcgtcgcgg tgccggtatt atttgcggaa ttggtcaacg aggcgctgat
    17521 ggtcagttca ccgttaccca gcgcctgcaa aacgttcgcc ggtacgctga cgctccagct
    17581 taaatctgac tgcacggtgg cgttatattg attgccgcca atggtgacaa tcaccgtatt
    17641 cccggcaacc gccgttcccg tgacctgacc gctgatggtc tgcgccacaa cgatttcagc
    17701 ggcgttgata atgtcatcgc cggagacggt gttaatggtc agcgtcgggc cgctaagatc
    17761 aacggtcaca tcgtgagtcg tgctgtcgct gttgcccgcg cgatcgctca ggctggcggt
    17821 gatggtctgg ctaccggtcg ccagcgccgt gacatccgcc gccggaatgc ccacgctcca
    17881 gttaccggag gcatccagcg tggtggtgta gtttttaccg ttcagcgtga cggttaccac
    17941 atcacccgcc gccgcgcccg tactggtgcc gctgaccacc aacgcctgac catgttcgat
    18001 ggcgttgata atgtcatcgc ccgcgacggt gttaatggtg agatccggcg cagtgatatc
    18061 taccaccagc gcgtggtcgg cgcttgccgg gttacccgct ttgtcgctca ccgtggcggt
    18121 cacggtgtaa ctgctggcgg ttaatccgct taaatccgct gccggaacat tcacgctcca
    18181 ggtgccatcc gccagaacgg tggtctggta cgtgttgtta ttgagcgtga cggtgagcgt
    18241 ctgcccggcc tgcgccgtgg tagtgccgct gatggtgatc cccgcgccag cttcgctcac
    18301 gttaaggata tcgtcgctgg cgatggtgtt gatggtcaca agcggcgcgg tggcatcaac
    18361 gctatacgcg cgatcggcct gggcgctgtt gccgttaata ttactgacac tcgcctgcac
    18421 gttgcccgcg ccgtccggta gcgcagcgag atcggcaggc ggcacattca ccgtccagct
    18481 accgttagct tccactgtgg tggtgtaatt tttgccgcca aaggtgacgg tcaccgtctg
    18541 cccggcttcg acaccgctgg tgctgccgct tacgaccagc gtttcgcctt tttcgacggc
    18601 gttgatcaca tcatcaccag agagagtgtt aatggtgatg gcgaccccag ccagatcgac
    18661 ggtaaccgga tgggtaatgg tggaggttgt tccggcactg ttggtgccgc ttaccgtgat
    18721 attcaccgta cccgctggcc agttgctgac gtctaccgcc ggaacaccga ggctccacgt
    18781 gccgtcggct aataccgtcg cgccataagt aacgttgtta atctcgaccg ttaacgccgt
    18841 tcccgccgtt aatccgctgc tgctgccggt gatcaccagc gcctgattgt gctcaatgct
    18901 gttaataaca tcatcgcccg ccacggtatc gacccgcagg ccaggcagat tggcgtcgat
    18961 cgtgatatcg cgcgagccgc tgccggtgtt gccgacgcca ttggtgaccg aggcattaac
    19021 cgtcaggtcg ccgttgccca gcgcctgaat atccgccgcc ggaacgtcca cactccagct
    19081 taaattagac tgcaccgtgg cggtgtaggt gttgccgccc agcgtaacgg taaccgtatc
    19141 cccctgcgct gcgcccgtca cctgcccgct gatggtttgc gcatttcccg attcggcagc
    19201 gttaataata tcgtcagtcg ccaccgcgtt aatggtaacg gcaggcaagg cgctgttgac
    19261 cagcacatta tgactggcgg aattactgtt gcctgccgta tcagtgacgt ttgccgttac
    19321 cgtgtagttg gcttcaccca atgcgctaac cgctgacgct ggcaccgtgg cgctccagtt
    19381 gccgttgcta tcggtagtag cggtgtaatt ttgcccgttc agcgtcaccg taatggtggt
    19441 gcccgcaggc tgattactgg tgccggtgat ttgcaggtcc gcgccttttt ccgtggcttt
    19501 aatcacgtca tcggtggcga tggtgttgat accaatggtt ggcgcggcga gattcacggt
    19561 gaccgtgcgg ctggcgtcat cgctgttgcc tgccgcgtcg gtaattgccg cagtgatagt
    19621 ttgcgggccg ctgccaagcg cagtgacgtc agccgccgga acgccgacgc tccagttgcc
    19681 ggaagcgtcc agcatggtgg tgtaggtttt actgtttagt gtgacggtga tgacatcacc
    19741 cgcttcgccg ccagtgctgg agccggagat caccagcgcc tgtccgtgtt cggcggcgtt
    19801 aataatgtca tcgccggaga cggtgttgat ggtcagcacc ggcacggtga gatccaccgc
    19861 cagcccgtgg ttagcggacg ccgggttacc cgctttatcg cttaccgagg cactaacggt
    19921 gtactggctg gcggtcagat tgctgagatc cgccgtcggt aagctgacgc tccagctgcc
    19981 gtccgcctgg acggagccgc tgtaagtcac accattaagc gtgacggtta ccgtctgccc
    20041 cgcttcggcg gtgctgctac cgctgatggt taacggattg cccgcctcgg cagcgttcag
    20101 aatatcgtcg gtggcgatgg tgttaatggc aagcgtcggg gccgtggcat cgacgctgta
    20161 ggcgtgggtt gccgaagccg tgttgccgtt aatagtgctg acgctggcct gcacggtggc
    20221 gtcgccgtcg cgtaacacgc tgagatcggc ggcgggtacg gtggttgtcc agctaccatc
    20281 gcccgctacg gtagcggtgt aggttttccc gccaaaggta acggtgaccg tttgccccac
    20341 ttccacgccg gaggtgctgc cggaaagggt taaatctgcc cctttttcgg cggcgttaat
    20401 cacatcgtcg ccggaaacgg tgttaatgga aatcgccacc gccgccagat cgacggtgac
    20461 cggatgggtg atggtggatg ttgttccggc tgtgttagtg ccacttaccg tgatattcac
    20521 cgtacccgca ggccagttac tcacgtctgc cgccggaaca ccaacgctcc atgtgccatc
    20581 ggctaatact gttgcagcgt aagtgaccgt gttaatcacc accgtcagcg ccgcgcccgc
    20641 cgccagcccg ctgctgctac cagttatcac cagcgcctga gcgtgctcga tgctattgac
    20701 cacatcatcg cccgccacgg tgtcaacgcg cagacctggc aggttggcgt cgatagtaat
    20761 atctcgcgaa ccgctgccag tattgccaac gccgttggtc accgaagcgt taaccgtcag
    20821 attgccattg ccgatggcct gaatatccgc cgccggaacg tccacgctcc agcttaaatt
    20881 cccctgcacg gtagcggtgt aagttttccc gccaagcgtt acggtaaccg tatcgcccgc
    20941 cgccgccccc gtcacctggc cgctgatggt ttgcgcattg cccgattcgg cggcgttaat
    21001 aatatcgtcg gtcgcaactg ggttaatggt gacgccaggc agcgcggtat tgacctgcac
    21061 gttatggctg gcggaattgc tgttgcctgc gctgtcggtg acgttcgccg tcaccgtgta
    21121 gctggcttcg cccagcgcac ctactgcgga agctggcacc gtagtgctcc agttgcctgc
    21181 agcgtccgtc gtcgcggtgt aattttgccc gttaagcgcc agcgtaatcg tggtgcctgc
    21241 tggctgatta ctggtgccgg aaatttgcag atccgcgcct ttttcgctgg cgttaattat
    21301 gtcatcggtg gcgatggtgt tgatgtcaat ggttggtgcg gtgaggttaa cggtgaccgt
    21361 gtgtgtctaa ttgtcgctgt tgcctgccgc atcggtaacc gtggcggtga cagtttgcgg
    21421 gccgctgcca agcgccgtga catccgccgc cggaacgccg acgctccagt tgccggaggc
    21481 gtccagggtg gtggtgtagg ttttactgtt tagcgtgacg gtgacgacat cccccgcttc
    21541 tccgccggtg ctggaaccgg aaatcaccag cgcctgcccg tgttcggtgg cgttaataat
    21601 gtcatcgccc gcaatggtgt tgatggtcag caccggaacg gtgagatcca ccgccagatt
    21661 atgcgtggtg ctcgccggat tgcccgcttt gtcattgacc gtggcggtca gggtgtggct
    21721 gccatcggtc aatgcctcaa gatctgccgc cggaatattg atgctccagc tgccgtccgc
    21781 ctgcaccgtt gcctgatagg tctgcacggt cgggctgtta agcgttatcg taacaatctg
    21841 ccccgcttcg gcggtggtac tgccgctcac cgtcacgccc gcatcggctt cgctggcgtt
    21901 gacgatattg tcgctggcga cggtattgat aatgatggtt ggcgcgctgc tgtcgacgct
    21961 gtagttgtgc accgccgagg cgctattgcc gttgatattg ctgacgctgg ccagtgcgga
    22021 ggcgctgccc tcgggtaatg acgccagatc ggcggcgggt acggtggcag tccagctacc
    22081 atcgctcgct acgctggcag tgtaattttt cccgccaaag gtgacggtga cggtttgacc
    22141 gggttctacg ttagtggtgg tgccggaaag ggttaaatca gcgccttttt ctgcggcgtt
    22201 aatcacatcg tccgtggcga tggtgttgat ggtgatcgct gccggagtga gatccaccgt
    22261 caccggatgc gtaatgctca ccgagtttcc ggcgctactt tcccctgaaa cggcaatatt
    22321 aaccgtcccc gcaggccagg cgctaacctg cgccgccgtg acgcccacgc tccagctacc
    22381 atcggcctgc accgcagtgg tgtattccac attattaatc gtaacggtaa gcggcgtgct
    22441 ttcagccagc cccgagctgc tgccggtgac caccagcgcc tgcccgtgct cgatgatatt
    22501 gaccacatca tcgcccgcca ccgtatcgac ccgcaggccg ggcagattgg cgtcgatggt
    22561 gatatcccgc gtgccgctgc cggtgttgcc gttttgattg gtgactgagg cattaaccgt
    22621 taaatcgcca tttcccagcg cctgaatatc tgccgctgga acgtccacgc tccaggtgag
    22681 attgctgccc accgtcgccg tataagtatt accacccaac gtaatagtta ccgtgtcgcc
    22741 atcttccgcg ccagtcacct gcccgctgat ggtttgcgcc acgcccgctt cggcggcgtt
    22801 aataatatcg tcggttgcca ccggattaat ggtcacaccg ggcagcgcgc tgtcgaccag
    22861 cacgttatgg ctggcagtgg cgctgttgcc gatatcgctg gtcaccgccg ccgttaccgt
    22921 atagttggcc tggcctaatg ctgtaaccgc cgatgccgga acggtgacgc tccagttgcc
    22981 ggaggcgtcg gtcgtggtgg tgtaattttg cccgttcagc gtcacggtga tggcggtgtt
    23041 aacaggctga tcgctggtgc cggtaatctg gagatcggcc cctttttcgc tggcgttaat
    23101 cacatcatcg cccgcaatgc tgttaatgcc gataaggggc gcggcggtat tgaccgtgac
    23161 ggtcaatgac tggctgcccg tgtttccggc tttgtcggtt accgagacgc tgacaggata
    23221 actgccgtcc gccagcccac tgaccaccga ggccggaacg cccagactcc agttgccgga
    23281 accatccacc accgtggtgt aatccacatt attcagcgtc acggtgacga tatcgcccgc
    23341 ctgagcgcca gtaaccgtgc cgctgatgat ttgcgcctga gcatgttccg gcgtgttgat
    23401 gacgttatcg gtcgccaccg gattaatggt taccaccggc ggcgtggcat ccaccagcgc
    23461 cacgcgtgaa acgctgctcg gattaccggc gacatcgttc acgctggcgg tgaccgtagc
    23521 gttgccgtca gccagtgcgc ccacgttggc ggcgggcaca gtcacgctcc aggtgccgtc
    23581 tggctgcacg acgccctgat acgtctgccc atttaacgta acggtcaccg tctgccccgg
    23641 ctgagcgtcg gtggtgccgg tgatgggcag atcctgcccg ctttcggcgg cgttaatgat
    23701 gttatcagcg gtcagtggat caatgcttaa ggccggggcc tggctatcga cggtaatggt
    23761 gcgggaagtg ttgccggtat tgccgctgag atccgtcaca gaagcggtaa tcgccaccgc
    23821 gccgtcaggc agatttccca tcgcggcggc aggtaccgtc agactccagg agccatcggc
    23881 ctgcacggta gtggtaaaac tctgtccagc cagggttact gtgacggttt gtcctgtctc
    23941 agcctgttga gtgctaccgg taagggtcag tggcgcacct ttttctgcgc cactgataat
    24001 gtcatcgccg gagacggtgt taatggtgat caacggagaa acaccgacca gcgtgattgc
    24061 tcgagacgcc gcgccagtat ttcctgcagc atctgtcacg gtgacgaaaa tcgtattggc
    24121 tccgcgatcc agcgtgcggg ttaccgctgg gtccagcgcc acattccagc tgccatctgc
    24181 cagcacgatg cccgtcagga catgagtgcc caatttcacc gttaccgtat cgcctggaga
    24241 agcaccgctg acttgtccgg agatgatctg cgcgacggcc tgctcggcat tattaagaat
    24301 atcgtcgccc gccacggtat taacactgac cacgggtgct gaggtgtcta ccgtgaaatt
    24361 cgcgctgtgg ctggtggtgt tccctgcccg atcgctgaca ctggcattga cagcgtaatt
    24421 accctccgcc agtgcctgga cttccgtggc aggcagcgtc acttgccagg ttccgtcgct
    24481 accgacggtt gctgtatggc ttttcccgtt gacggtcagt gttacggttt gccccgcttc
    24541 tgcattcgac gtgccgctca gtaccagcgc gacgttatgt tccgcagcac tgataatgtt
    24601 gtcctgagcg atggtgttga tggcaagcgt tggcggttgg gtatcgaccg ttagtagctg
    24661 cgcgccggtg accgtattgc ctgtggcgtc tttcccactg accagcaccg cctgattccc
    24721 ttcgcctaac gcctgtgcat cggcggtcgg cacctgcacg ctccaggcac cgttagcgcc
    24781 tacgctggtc gtgtaggttt tgccgtttag cgtaacggtg agttctgttc cctgcgccag
    24841 atggctactg gtgccagaaa gcgtaaaccc ggcggcctgt tcgctggcgt taataatgtt
    24901 atcgcctgcg gtggtggcaa cctgcacgct ggcctgacta ttgtcgatgg ttaacgttat
    24961 cgaaccggcg tcagtgatgg tactgccgac aatttgcacc actgaccagg tatgttcacc
    25021 tgcactttgc gtgggtaacg agacactcca gtgaccgttg ttgtctacct gagtactggc
    25081 aatagtattg ccgttactgt ctttaatctg aatagtcgcc cccgcctgcc cgctgccgct
    25141 aaaggttggc gtggtgtcat cggtgatgtc attggtggct agaatgccgc gctgatcgcc
    25201 ctgattatcg gtaactaaaa atgatggtgt ggcgttgcca ggctcagcag gtggcgtagg
    25261 gttattaatc acttctgttt tcgagtcgcc atcgccaccg cttgccagca gtgcaccaag
    25321 cgcgccacca cctaccgccg cccccgccag ccaaccccac gggaaggcgc tggtctgagc
    25381 aacggtatca agaaatggcg caatgctttc aatcgcagtg gtctgggcag tcagttctac
    25441 gggggctaat ccacccgcag cagtatcggc aaaggtgata tgggttagct gttgaccatc
    25501 ggcaaacacc agttccgatt gttcagctgt attcgccgct tgcaggaaat aaccgttgca
    25561 gcggatcacc gtgccgtcct gcatatagat cagcaggtca tttccttcac ggacgtaacg
    25621 cgcaacggca gaggcctgag catgaacctg caccacggaa ggctcataaa taacaaccgt
    25681 gacgtcgcca ggattaatca gcgtttgttt gacggatgtt ttacgcgaaa taacatcaat
    25741 aatcaggctc ataatggcat tcctgataaa tggcgcacat ccttagcgag tccatttatc
    25801 taaataagac aaaggtaatc acttaacaac ggcccactat taataatggc aggattattt
    25861 caagtcgggt aagtcgttgt ataagccctg tttaattccc gctaatggaa taaggttatt
    25921 caccgcagcg gcataattta cggcggcgac ccagccatca taattggcat ttatttcagc
    25981 cgattgcgcc tgaaagacat cttgttcgac ggttaataga tcattcagac tgcgtttacc
    26041 caatttatat tcattttggt acacatctcg cgttttgtgc gcactttcgg attgcgctaa
    26101 accggcttct tcacgacccc gtgcgccggt ccagttcgca tatgccacag acgctcgttg
    26161 cagcacatcc agttttgcct gttcgacctg cgaggcagag attttttgtt gcccttcggc
    26221 ctgttgcacc tgggcagaaa ccgcgccgcc ctgatacagc ggcgcgttaa cgttcagttg
    26281 tagctgatca tcccaatacg agcggtcgct ggtctggtag cgcgttttac ccccctgaat
    26341 actgagcgtt ggccagtatt gcgctttcgt tttttccacg ccgtactgtg ctgactggcg
    26401 taagttttct gccgccagca ccagcgggat ggactggtaa tcaatattct tcagcgatac
    26461 cggctgctcg gctaattcag caggtggcgc ggctatcgcc tccggctgta cgccagtgag
    26521 caccgccagt tgcgctttgg cgctcgccat ctgcgcctga tattgctcca gcgttgagcg
    26581 cattccggca atacgcgtct gggcctgtaa ttcatccgac gatgagttca ggcctgcgtt
    26641 agcgcgcaat gccgccatgt tgtagacgtt ttccagcgag tgaatattgc gttgagccgc
    26701 atcgcacaaa gcctgataac gactgacttc catgtaggca gtcgccgttt tctctgcaac
    26761 atcggttaag gttgccatca atttaaagcg gtagctgtca cgggcggcag tttgcagatt
    26821 gatatcgttg ttagttttac caaagtcata caccagttgt gtcagggtta tgccatacga
    26881 aacgttgtta tcaagcctgc cgctggagtc cgtttgtcgg gacggccccg cgttgccggt
    26941 taatccaacg tgcggatacc aggcactttt ggcttcatca atttgcgctt cgccaatacc
    27001 tatctgcgcc gcctgttgag tgacaccggg atttcgcgca aaggcgcgta atatactttc
    27061 ctgtaaagat aattgggcaa ctggcgttac cgctggggca ttcgcccatt gtggcagcgg
    27121 tgcagcaatt gcagcttctc ccatcaccat aaaacttata aaaaaaataa gtccattatt
    27181 tttacggttt ttaaactgga atcgcgccat agataagtca cttccttcca ggtcattatt
    27241 ttaactggtt tttataacca gtctcgtttt attaagaagg gtaataactt gtgcatctct
    27301 tttgtcgatc attgatatgg gcaaagaaat gcagctaaaa agcttatacg ttatttattt
    27361 agaatacact acgactttta gattaccatc atacacatt
//
LOCUS       DAZFKY010000051        27107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFKY010000051 DAZFKY010000000
VERSION     DAZFKY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(171..1835)
                     /gene="glnS"
                     /locus_tag="ACHKCL_001877"
     CDS             complement(171..1835)
                     /gene="glnS"
                     /locus_tag="ACHKCL_001877"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5762856.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2038..3984)
                     /gene="nagE"
                     /locus_tag="ACHKCL_001878"
     CDS             complement(2038..3984)
                     /gene="nagE"
                     /locus_tag="ACHKCL_001878"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5762857.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4317..5117
                     /gene="nagB"
                     /locus_tag="ACHKCL_001879"
     CDS             4317..5117
                     /gene="nagB"
                     /locus_tag="ACHKCL_001879"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5762858.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5177..6325
                     /gene="nagA"
                     /locus_tag="ACHKCL_001880"
     CDS             5177..6325
                     /gene="nagA"
                     /locus_tag="ACHKCL_001880"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5762859.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6334..7554
                     /gene="nagC"
                     /locus_tag="ACHKCL_001881"
                     /gene_synonym="nagR"
     CDS             6334..7554
                     /gene="nagC"
                     /locus_tag="ACHKCL_001881"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5762860.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7602..8354
                     /gene="nagD"
                     /locus_tag="ACHKCL_001882"
     CDS             7602..8354
                     /gene="nagD"
                     /locus_tag="ACHKCL_001882"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5762861.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8611..10275
                     /gene="asnB"
                     /locus_tag="ACHKCL_001883"
     CDS             8611..10275
                     /gene="asnB"
                     /locus_tag="ACHKCL_001883"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5762862.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10655..10731
                     /locus_tag="ACHKCL_001884"
     tRNA            10655..10731
                     /locus_tag="ACHKCL_001884"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10689..10691,aa:Met,seq:cat)
     gene            10742..10826
                     /locus_tag="ACHKCL_001885"
     tRNA            10742..10826
                     /locus_tag="ACHKCL_001885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10776..10778,aa:Leu,seq:tag)
     gene            10850..10924
                     /locus_tag="ACHKCL_001886"
     tRNA            10850..10924
                     /locus_tag="ACHKCL_001886"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10882..10884,aa:Gln,seq:ttg)
     gene            10959..11033
                     /locus_tag="ACHKCL_001887"
     tRNA            10959..11033
                     /locus_tag="ACHKCL_001887"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10991..10993,aa:Gln,seq:ttg)
     gene            11049..11125
                     /locus_tag="ACHKCL_001888"
     tRNA            11049..11125
                     /locus_tag="ACHKCL_001888"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11083..11085,aa:Met,seq:cat)
     gene            11174..11248
                     /locus_tag="ACHKCL_001889"
     tRNA            11174..11248
                     /locus_tag="ACHKCL_001889"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11206..11208,aa:Gln,seq:ctg)
     gene            11286..11360
                     /locus_tag="ACHKCL_001890"
     tRNA            11286..11360
                     /locus_tag="ACHKCL_001890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11318..11320,aa:Gln,seq:ctg)
     gene            complement(11514..12689)
                     /gene="ubiF"
                     /locus_tag="ACHKCL_001891"
     CDS             complement(11514..12689)
                     /gene="ubiF"
                     /locus_tag="ACHKCL_001891"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5762863.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12835..14259
                     /gene="miaB"
                     /locus_tag="ACHKCL_001892"
     CDS             12835..14259
                     /gene="miaB"
                     /locus_tag="ACHKCL_001892"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5762864.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14412..15452
                     /locus_tag="ACHKCL_001893"
     CDS             14412..15452
                     /locus_tag="ACHKCL_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5762865.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15449..15916
                     /gene="ybeY"
                     /locus_tag="ACHKCL_001894"
     CDS             15449..15916
                     /gene="ybeY"
                     /locus_tag="ACHKCL_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5762866.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16006..16884
                     /gene="corC"
                     /locus_tag="ACHKCL_001895"
     CDS             16006..16884
                     /gene="corC"
                     /locus_tag="ACHKCL_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5762867.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16909..18447
                     /gene="lnt"
                     /locus_tag="ACHKCL_001896"
     CDS             16909..18447
                     /gene="lnt"
                     /locus_tag="ACHKCL_001896"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5762868.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18844..19752
                     /gene="gltI"
                     /locus_tag="ACHKCL_001897"
     CDS             18844..19752
                     /gene="gltI"
                     /locus_tag="ACHKCL_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5762869.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19922..20662
                     /gene="gltJ"
                     /locus_tag="ACHKCL_001898"
     CDS             19922..20662
                     /gene="gltJ"
                     /locus_tag="ACHKCL_001898"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5762870.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20662..21336
                     /gene="gltK"
                     /locus_tag="ACHKCL_001899"
     CDS             20662..21336
                     /gene="gltK"
                     /locus_tag="ACHKCL_001899"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5762871.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21336..22061
                     /gene="gltL"
                     /locus_tag="ACHKCL_001900"
     CDS             21336..22061
                     /gene="gltL"
                     /locus_tag="ACHKCL_001900"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5762872.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22179..23114
                     /gene="rihA"
                     /locus_tag="ACHKCL_001901"
     CDS             22179..23114
                     /gene="rihA"
                     /locus_tag="ACHKCL_001901"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5762873.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23198..24868
                     /gene="hscC"
                     /locus_tag="ACHKCL_001902"
     CDS             23198..24868
                     /gene="hscC"
                     /locus_tag="ACHKCL_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO5762874.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24928..26379)
                     /gene="djlC"
                     /locus_tag="ACHKCL_001903"
                     /pseudo
     CDS             complement(24928..26379)
                     /gene="djlC"
                     /locus_tag="ACHKCL_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26376..27083)
                     /locus_tag="ACHKCL_001904"
     CDS             complement(26376..27083)
                     /locus_tag="ACHKCL_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5762875.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         6650 a         7040 c         6887 g         6530 t
ORIGIN      
        1 gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa ctcaatatat tgcagagatc
       61 atgtaggcct gataagcgta gcgcatcagg caatttagcg tttgaaactg tttgtctgtt
      121 ccttgccgga tgcgacgtaa acgccccatc cggcatagcg aaacttaaaa ttactcgcct
      181 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttccgccgta
      241 gaatggcggc tatcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      301 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      361 atcaccgaca ggaaatcatc cgcagcacct gggttcggca cgctgaacag acggtcatac
      421 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      481 ttacgaccat ctgccggatc tttgcttaag gtatcggcgt cataagtaca gaagatggtg
      541 gtgatattac cttcggcatc tttctcgacg cgttctgcct taatcacata agcattacgc
      601 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      661 tcggagcgat caatccaaat ctcaccgcta aacggcacct gacggctccc catttccggt
      721 ttgttcggat ggttcggcat ggtaaccatt tcgccttcgc cctgatagtt ttcgataacc
      781 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      841 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      901 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      961 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcaa gttcagctta
     1021 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcgggtga
     1081 acaggaatgg tgatgttgtc cagtacccag tcgtacagac gacggttatc ctggaactca
     1141 agcgtacaca gagagtgcgt aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg
     1201 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1261 tacagcaccg gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg
     1321 caggctttac cttcttcaaa accgccggta cgcattttct cgaacagcgc caggttctct
     1381 tcaacgctac ggtcgcggta cgggctgttt ttaccaggct gcgtcagggt gccgcggtat
     1441 tcgcggatct gttccggcgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1501 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1561 tgaaaaccta accactcaac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1621 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1681 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1741 aaacgggtgt gtactgtggt gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1801 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1861 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tagtgaaaat
     1921 cgaaaatctc actgagatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1981 ccaccgcttg tcattgttgg atgcgacgct caagcgtcgc atcaggcata aagcagatta
     2041 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgat
     2101 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg aacgggcgtt
     2161 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcgcc
     2221 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtatcga tacccatgtg
     2281 aacgacaatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2341 tttcacgatt gttcctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2401 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2461 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2521 ttttgctaca ggagcggcag ttgccggagt tgcttcagca gaggcagctg ctaccgggcc
     2581 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2641 ctgaatagtc tgtttgttca gttttactac cccagaagcc cccagacgtt tacacatcgt
     2701 atcgttaacg cgggcagagt cagccacggt aaggcgcaga cgggtgatac aggcgtcaat
     2761 tgctttcagg ttgtcagtgc cgccaaccgc agcaatatag ttggtcgcca gttgattgag
     2821 gccttcttca gtgttgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2881 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2941 gaagaagaca acacccataa ccagcagcat ccagacgttc tggctggcgg ccggcaggtt
     3001 atacatcaac gcgtagtcga tagcccccgc agagaaagag aagccggcat ggatacccag
     3061 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3121 agcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3181 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3241 catcgccagc gccgcacccg gcaggccgaa catcatgatc gggaagaagc cggacatgaa
     3301 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3361 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3421 accggttggg atcaacagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3481 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3541 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3601 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3661 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3721 caccatcgct ttggttaaca caaagtaacc taccgccccc gccagcgccg ccgcacctgc
     3781 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3841 aatcgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3901 cagcaacagt gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3961 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     4021 aaagccgtat ttttattgtg ctgcaaattg tactgccgat gttctgtaat cagattgtta
     4081 gatcatctgc cacagagtgt gtgaaaattt aattcgtatc gcaaattaaa cgagtgtctt
     4141 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4201 acttatttta tcattcaaaa aatcaggtcg gattgacgcc tgtctgcgca aatccaggtt
     4261 acgcttaaag atgcctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4321 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4381 atcgtatcaa tgcgttcaaa ccgactgctg atcgtccgtt tgttctgggc ctgccaactg
     4441 gcggcacgcc aatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4501 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4561 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4621 aaaacatcaa ccttctcaac ggcaacgccc cggatatcga cgccgagtgc cgccagtatg
     4681 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4741 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4801 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4861 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgattc
     4921 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc agttgaaggt tgcgtgaacc
     4981 atatgtggac catcagctgt ctgcaactgc atccgaaagc catcatggtg tgcgatgagc
     5041 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5101 atatcaaagg tctgtaattg ttatccctgc cctggctcct tgctcagggc aatatttttt
     5161 aaaatcgggg gtcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5221 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5281 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5341 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5401 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5461 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5521 cgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gcccgtggct
     5581 gaatctggtg aaaaaaggta cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5641 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5701 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5761 ctccaacgcg acgttgaaag aagcgaaagc cggtttccgc gcggggatta cctttgccac
     5821 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5881 cctcgatgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5941 caacattcgt aatgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     6001 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6061 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6121 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6181 gacgctctat ccggcgcgtg cgattggcgt tgagaaacgt ctcggcacac tcgccgcagg
     6241 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6301 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6361 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6421 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6481 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6541 tccaccgggg gccgccgcgc tatctctatc gtcaccgaaa cccgcaattt ccacgcaatc
     6601 ggcgtacggc ttggtcgtca cgacgccacc atcaccctgt ttgatctcag cagcaaagtg
     6661 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6721 ctgaatgcca ttgctcagtt tattgatagc taccagcgca aactgcgcga gctgatcgcc
     6781 atttccgtta tcctgccagg acttgttgac ccggacagcg gcaaaattca ttacatgccg
     6841 catattcagg tagaaaactg ggggctggta gaagcactgg aagagcgttt taaagtgacc
     6901 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6961 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     7021 tctaacgggc gcatttttat tggccgcaac ggcaacgtcg gtgaaattgg ccatattcag
     7081 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgct
     7141 gccaacgccg ctattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgc
     7201 gtgccgctgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7261 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gcaaaaccat cgccattgct
     7321 atcaacttat ttaatccgca aaaaattgtt attgccggtg aaatcaccga agccgataaa
     7381 gtgctgctcc ctgctattga aagctgcatt aatactcagg cgctgaaggc gtttcgtact
     7441 aatctgccgg tggtacgttc tgagctagac caccgctcgg caatcggtgc ttttgcgctg
     7501 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7561 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7621 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7681 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7741 agaccgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgta cctgacagcg
     7801 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag
     7861 cgtatgttgt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7921 ccgatgtgaa ccctgatttt gtgattgttg gcgaaacgcg ttcctacaac tgggacatga
     7981 tgcataaagc ggcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     8041 cccacgggcg cggtttttat ccggcctgtg gcgcgttgtg tgcagggatt gagaaaatct
     8101 ccgggcgcaa accgttctat gttggtaagc cgagtccgtg gattatccgc gcggcattaa
     8161 acaaaatgca ggcacattcg gaagaaacgg tgattgtcgg cgataacctg cgtaccgata
     8221 ttctggccgg cttccaggca ggtctggaga cgattctggt gctttctggt gtttcgtcgc
     8281 tcgacgatat cgacagtatg cctttccgcc ccagctggat ttacccgtca gtcgctgaaa
     8341 tcgacgttat ctgaatagca accacgtctc aggacgtggt ttcttatttt tacccatcat
     8401 taaaatcatc ccattcaata aaatccgcca aaaattatcg atccatcaat aaatacatca
     8461 ccgcaccctc ccctttttca caaatcagca atcaccatta cgttttttat tttttcaccg
     8521 ccaaatcgct tgcgcacagt gctgctttgc ggcatttttt taaacaagca aacacaacaa
     8581 gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatc
     8641 aaaacagacg cagttgagct gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat
     8701 cgtggcccgg actggtccgg tatttatgcc agcgataaag ccattctcgc tcacgaacgt
     8761 ctgtcaattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaacccac
     8821 gtactggcgg taaacggtga aatctacaat caccaggcat tgcgcgctga atatggcgat
     8881 cgttatcagt tccagacagg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa
     8941 gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa
     9001 aaagatgcct acctgattgg tcgcgaccat ctggggatca tcccactgta tatgggctat
     9061 gacgaacacg gtcagctgta tgtggcctca gaaatgaaag ccctggtgcc agtttgccgc
     9121 acgattaaag agttcccggc ggggagctat ttgtggagtc aggacggcga aattcgttct
     9181 tattatcatc gcgactggtt cgactttgat gcagtgaaag ataacgtgac cgacaaaaac
     9241 gagctgcgtc aggctctgga agattccgtt aaaagccatc tgatgtctga tgtgccttac
     9301 ggtgtgctgc tttctggtgg tctggattcc tcaattattt ccgctatcac caagaaatac
     9361 gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gttacactcc
     9421 tttgctgtag gtctgccggg ttcaccggat ctgaaagcag cccaggaagt ggcaaaccat
     9481 ctgggcactg tgcatcatga aattcacttc actgtacagg aaggtctgga cgccatccgc
     9541 gacgtgattt accacatcga aacctatgat gtgaccacta ttcgcgcttc aacaccgatg
     9601 tatttaatgt cgcgtaagat caaagcgatg ggcattaaaa tggtgctgtc cggtgaaggt
     9661 tctgatgaag tgttcggcgg ttatctttac ttccacaaag caccgaatgc caaagaactg
     9721 catgaagaga cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc gcgtgccaac
     9781 aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa gaaattcctc
     9841 gacgtggcga tgcgcattaa cccgcaggat aaaatgtgcg gtaacggcaa gatggaaaaa
     9901 cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa
     9961 gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcggcg
    10021 cagcaggttt ctgatcaaca gctggaaact gcccgcttcc gcttcccgta caacacaccg
    10081 acctcaaaag aagcgtatct gtaccgggag atctttgaag aactattccc gcttccgagc
    10141 gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt cttccgctaa agcgatcgaa
    10201 tgggatgaag cgttcaagaa aatggacgat ccgtctggtc gcgcggttgg tgttcaccag
    10261 tcggcgtata agtaagaaat gcgaaacagc cccggtgaaa tccggggctg tttcagttat
    10321 tgaggtgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc cacatcactg
    10381 tttgacaact catcactgta ggccagtatt tcatagatta atttcccacc actattgtta
    10441 aattccgaat ttcatcctgt atgacaaaag aatggctgta gggtgggaaa aaacacaaag
    10501 taaacaataa ttgacgaata tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt
    10561 cgagcaattt tccttgaaaa agaggttgac gctgcaaggc tctatacgca taatgcgccc
    10621 cgcaacgccg ataaggtatc gcgaaaaaaa agatggctac gtagctcagt tggttagagc
    10681 acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc atcttttttt
    10741 tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc
    10801 gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggtatcgc
    10861 caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca
    10921 gccatcttct ttgagtaagc ggttcaccgc ccggttattg gggtatcgcc aagcggtaag
    10981 gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcgaaga
    11041 aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    11101 ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc ggcaaattca
    11161 aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc
    11221 cgaggttcga atcctcgtac cccagccaat ttattcaaga cgcttacctt gtaagtgcac
    11281 ccagttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    11341 gaatcctcgt accccagcca cattaaaaaa gctcgcttcg gcgagctttt tgcttttctg
    11401 cgttcactca atgtcgaatg cgatgttgac acgtcttatc cttcaatgtc ggatgcgacg
    11461 ctgccgcgtc ttatccgacc tacggttggc acgcatccgg caatgttgta aggctacaac
    11521 cctaacgcat atttcagcgc ctgacgtttc aacacgccag cacgctccgc cgccatcaaa
    11581 ccaagattac gcacaaaacg cagcggcggc agattattac tgaatccggc ataaaacaga
    11641 tccataccgc tctgcataat gaagttatcc gccatgcgcc gcatctggta acgcttgagg
    11701 acaggataac tggcccacgc ttcgccgtag ctgcgggcat tcaccagaac atcaatcagg
    11761 gcatcgacat cacgataacc aagattcacc ccctgccccg ccagcggatg gatggtgtgc
    11821 gcggcatcac ccaccagcgc aagccccggc tgcacatact gcaacgcatg gcggcgtgtc
    11881 agcggaaacg caccagcggc aagcggtgta acgtaaccca gacgcgacgg gaaatgcttc
    11941 gcgatttcca cctgtaactg cgccatattc atattctgca actggcgaat gcgcgccgga
    12001 gagtcgtacc acaccagcga tgcccagtta tcaaacagcg gcagaaacgc gcgcggtcca
    12061 tccggggtaa attgctgcca ggtgctatcg ccgggatcgt tctcgcactg gacgctaatc
    12121 aacatgcacg actgcgcata ctgccatgca tgaacgccaa ttcccgccat ctgccgcacc
    12181 tgcgaatttg cgccgtcggc accaatcacc agcttcgcgc gaatcacttc accgcctttc
    12241 agctccagct cctgaagatc attatctcga tgtaatgcaa tcagcgaggt tggcacacgt
    12301 aacgttactt tcggatgcgc ttccagcgcc tgccacaatg cctgttgcag gacagtgttt
    12361 tctaccatat agccaagcaa cggcaacttt aattcggctg cgtcaaatac cacatgcgcc
    12421 gtttcccact cccacgtttc cagtctgcgg taaggatggc aacgcatagc ctgtactgca
    12481 tcccagaccc ctaacccttt aagcaatgat accgaagccg cgctgatcgc cgagatccgc
    12541 acgtccggtt ggctatcagc gacaaacggt gcaggttctg cgtgctcgat caccgttacc
    12601 gtaaatccgt gctgtgccag ccccagcgcc agtgcgccgc cgaccattcc tccgccgaca
    12661 atggcaattt ccgttggttg atttgtcatg gtcaatcatc ctgttagcaa tatgctttaa
    12721 gtttacagga tttttacccc caagggctga taacgctcat actggtcaca acggcagcaa
    12781 agcattacac tatgcgccct gcattcctgg ctactatttc gcaagagcaa gtcgatgacc
    12841 aaaaaactcc atattaaaac ctggggctgt cagatgaacg agtacgattc atcgaagatg
    12901 gccgatctgc tggatgccac ccacggctat caactgaccg acgtggcgga agaagcggat
    12961 gtgctgctgc tgaacacctg ctcaatccgc gagaaggctc aggaaaaagt cttccatcag
    13021 ttgggtcgct ggaaactgtt aaaagagaag aatccagacc tgattatcgg cgtcggtggc
    13081 tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg cccactatgt cgatattatt
    13141 tttgggccgc aaacgctgca ccgtctgccg gagatgatca actccgtgcg cggcgaccgc
    13201 agcccggttg tagatatcag cttcccggaa atcgagaagt ttgaccgtct accggaaccg
    13261 cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag gctgcaataa atattgcacc
    13321 tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc gtccgtctga cgatattctg
    13381 tttgagattg cccagcttgc ggctcagggc gtgcgtgaag tcaacctgct cggtcagaac
    13441 gtgaacgcct ggcgtggtga aaactacgac ggcaccaccg gatcgtttgc cgatctgttg
    13501 cgtctggttg ctgcgatcga cgggatcgat cgtattcgct ttaccaccag ccatccgatc
    13561 gaattcaccg acgatatcat cgaagtgtat cgcgacacgc cggagctggt gagcttcctg
    13621 catctgccgg tacagagcgg ttccgatcgc attctgaacc tgatgggacg tacccatacg
    13681 gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg cgcgtccaga tattcagatc
    13741 agttctgact tcatcgtcgg cttccctggc gaaaccaccg aagacttcga gaaaacgatg
    13801 aagctgattg ccgacgtcaa tttcgacatg agctacagct ttatcttctc tgcacgtccg
    13861 ggtacaccag ccgccgatat ggttgatgat gttccggaag aagagaagaa gcagcgtctg
    13921 tatattctgc aagaacgtat caatcagcaa gcgatggcgt ggagccgccg gatgctcggc
    13981 accacccagc gtattctggt agaaggtaca tcgcgtaaga gcatcatgga gctttccggg
    14041 cgtacggaaa ataaccgcgt ggtcaacttc gaaggcacgc cggatatgat cggtaaattc
    14101 gtcgatgtag aaattaccga cgtctacccg aactctctgc gcggtaaagt ggtgcgtact
    14161 gaagatgaaa tgggtctgcg cgtggcagaa acaccagaat cggtgattgc ccgtactcgc
    14221 aaagaaaacg accttggcgt gggttattat cagccgtaat tctcaggccc gccgttccgg
    14281 tgggccttgt tttttctctc tttatcccca tctttatcgc aatgcttgcg cctttttcct
    14341 gtggcgtgaa taatttcctt ataggccggt gaatgttcag cttccggcgg catacagata
    14401 agaggaacgg tttgaacata gacactcgcg aaatcaccct ggagccagca gacaacgcgc
    14461 gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa gcagctcgaa cgccgtctcg
    14521 gcatcgagat caatcgccgc gataaccact ttaaactgac cggccgtccg atttgcgtca
    14581 ccgctgcggc agacattctg cgtagcctgt atgtcgatac tgccccgatg cgcggtcaga
    14641 ttcaggatat cgaaccggaa cagatccacc ttgcgattaa agaagcacgg gtactggagc
    14701 aaagcgcgga gagcgtgccg gagtacggca aagcggtcaa tatcaaaacc aaacgcggcg
    14761 taattaagcc gcgcacgcca aaccaggcgc agtacatcgc caatattctc gaccatgaca
    14821 ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta cctggcagtg gctgcggcag
    14881 ttgatgccct ggagcgtcag gaaattcgcc gtattctgct gactcgtccg gcagtagaag
    14941 ccggtgagaa actgggcttc ctgcctggcg atttaagcca gaaagtagac ccgtatctgc
    15001 gcccgctgta cgacgcgctg tttgaaatgc tgggttttga gaaagtcgag aaactgattg
    15061 agcgcaacgt tattgaagtc gcaccgctgg cctatatgcg tggtcgtacg ctgaacgacg
    15121 catttatcat tctcgatgag agccagaaca ctaccatcga acagatgaag atgttcctga
    15181 cccgtatcgg ttttaactca aaagcggtta tcaccggcga cgtcacacag atcgacctgc
    15241 cgcgtaatac taaatcaggc ttacgtcatg ccatcgaagt gctggccgat gtcgaagaga
    15301 tcagctttaa cttcttccac agcgaagacg tggttcgtca cccggtggtg gcgcgtatcg
    15361 ttaacgccta tgaagcctgg gaagaagccg aacaaaaacg taaagcggcg ctggcggcag
    15421 aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg atcctcgatt tacaactggc
    15481 atgtgaagat aactccggat tgccggaaga gagccagttt caaacatggc tgaatgcggt
    15541 gatcccgcag tttcaggaag agtcggaagt gacgattcgc gtggtcgata ccgccgaaag
    15601 ccacagtctc aatctgacct atcgcggtaa ggataagccg accaacgtgc tctccttccc
    15661 gtttgaagtg ccgcctggca tggagatgtc gctactgggc gatctggtta tctgccgtca
    15721 gatggttgag aaggaagctc aggagcaagg caaaccactg gaggcgcact gggcgcatat
    15781 ggtggtgcac ggcagtctgc atttgttagg ttacgatcac atcgaagatg acgaagcaga
    15841 agaaatggaa gccctcgaaa cagagattat gcttgctctg ggctatgagg atccgtacat
    15901 tgccgagaaa gaataatttg ccagccattt gactggcaga cgtccccgcc gttggtgatt
    15961 aacgacggcg ttttgattaa ctaacatgag aacccatacg acgccatgag cgacgacaat
    16021 tcacacagta gtgacacgat aagcaacaag aagggatttt tctccctgtt actcagccaa
    16081 cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg cgctgatccg tgattccggg
    16141 cagaacgacc ttatcgacga agatacgcgc gatatgctcg aaggggtgat ggacatcgca
    16201 gaccaacgcg tccgcgacat catgatcccc cgctcccaga tgattaccct gaaacgcaac
    16261 cagacgctgg acgaatgcct tgatgtcatc atcgagtccg cccactcacg tttcccggtg
    16321 attagcgaag acaaagatca cattgaaggg attctgatgg cgaaagactt gctgccgttt
    16381 atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt tacgtcaggc ggttgtcgtt
    16441 cctgaaagta agcgcgtaga ccggatgctg aaagagtttc gctctcagcg ttaccacatg
    16501 gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg taaccattga agacatcctg
    16561 gaactgattg ttggtgagat tgaagacgaa tatgacgaag aagatgatat cgacttccgt
    16621 cagctgagtc gtcatacctg gaccgtgcgc gcactggctt ccattgaaga cttcaacgaa
    16681 gcgttcggca cccactttag cgatgaagaa gtcgacacta tcggtggtct ggtgatgcag
    16741 gcatttgggc atcttccggc acgtggcgaa accatcgaca tcgacggtta ccagttcaaa
    16801 gtggcgatgg ccgacagtcg gcgtattatt caggttcatg tcaaaatccc ggatgactca
    16861 ccccagccga agctggatga ataaaaccga aactggatag ataactacat ggcttttgcc
    16921 tcattaattg aacgccagcg cattcgcctg ctgctggcgt tattattcgg tgcctgcgga
    16981 acgctggcct tctctcctta cgacgtctgg cctgcggcga ttatttcgct gatggggctt
    17041 caggcgttga cctttaaccg ccgtccactc cagtctgccg ctattggctt ttgctgggga
    17101 tttggcctct ttggcagcgg tattaactgg gtctatgtca gcatcgcgac ctttggcgga
    17161 atgcctggcc cggttaacat cttcctggtg gtactgctgg cggcgtattt gtcgctgtat
    17221 accggactgt ttgctggcgt gctgtcgcgc ctgtggccga aaaccacctg gctgcgcgtg
    17281 gcgattgccg cccctgctct ctggcaagtg accgagtttc tgcgcggttg ggtactgaca
    17341 ggcttcccgt ggttacagtt cggctatagc cagattgatg gcccgttaaa agggctggca
    17401 ccgctaatgg gcgtggaagc cattaacttc ctgcttatga tggttagcgg cctgctggca
    17461 ctggcgttag tcaaacgcaa ctggcgtccg ctggtggtgg ccgtcgtgct gtttgccctt
    17521 cccttcccgc tgcgttacat ccggtggttt accccacaac cggagaaaac cattcaggtt
    17581 tcgatggttc agggcgatat tccgcaatcg ctgaaatggg acgaaggcca gcttcttaat
    17641 acgctgaaga tttactacaa cgcaacggca ccgctgatgg gcaaatcatc gttgattatc
    17701 tggccggagt cggcgataac cgatctggaa attaatcagc aaccgttcct caaagcactg
    17761 gacggtgagt tgcgtgataa aggtagctcg ctggtgaccg ggattgttga cgcgcgtctt
    17821 aataagcaga accgttacga tacctacaac accatcatca cactgggtaa aggtgcaccg
    17881 tacagctacg aatcagccga tcgctataac aaaaaccatc tggtgccgtt tggcgagttt
    17941 gtcccgctgg agtcgattct gcgtccgtta gcaccgttct ttgatctgcc gatgtcgtcg
    18001 ttcagccgtg ggccatatat ccagccgccg ctgtcggcaa atggtattga gcttactgcg
    18061 gctatttgct acgagatcat tctcggcgag caagtgcgcg ataacttccg cccggatacc
    18121 gactatctgc tgactatctc caacgatgcg tggtttggta agtctattgg tccatggcag
    18181 cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc gcccactgtt gcgcagcacc
    18241 aacaacggca ttacggcggt gattggcccg cagggtgaga ttcaggcgat gatcccgcag
    18301 ttcacccgcg aggtgttaac cactaacgtg acgccgacca ccggactcac accatacgca
    18361 cgtaccggca actggccgct gtgggtgctg acggcactgt ttggttttgc tgctgtgttg
    18421 atgagtctgc gtcagcgacg taaataatcc cttcttatcg tgccggaata catcccatat
    18481 ttcggcacgc tcaccggatg cggcatcagt gcattatccg gataacaata ttctcctatc
    18541 caactttggc acatctattg ctttgttata caaggcaaag ctttaaccag catcagtaca
    18601 acccggttgc accacagcaa tccaccggta gcaccaaaag agtgcatttt aagaattttg
    18661 cttcggaatg gtgcggcgca cctcccaaat ataaacaata aaacagcaat atctttacat
    18721 ttagccaaac taaatgttac ctaacaggca caacactaca caataaagtt gcagatgata
    18781 acaacacaaa cactcacaac gggtatccat gcgttcttaa cgcagaagat aaaggagttg
    18841 gatatgcaat tacgtaaacc tgccacagca atcctggccc tggcgctttc cgcaggactg
    18901 gcacaggcag atgacgccgc cccggcagcg ggcagtacgc tggacaaaat cgccaaaaac
    18961 ggcgtgattg tcgtcggtca ccgtgaatct tcagtacctt tctcttatta cgacaatcag
    19021 caaaaagtgg tgggttattc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag
    19081 aaactcaaca aaccggactt gcaggtgaaa ctgattccga ttacctcaca aaaccgtatt
    19141 ccgctgctgc aaaacggcac ttttgatttt gaatgtggtt ctaccaccaa caacgtcgaa
    19201 cgccaaaaac aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgttgacc
    19261 aaaaagggcg gcgatatcaa agattttgcc gacctgaaag gcaaagccgt agtcgtcact
    19321 tccggcacta cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg
    19381 cgcatcatca gcgccaaaga tcacggtgac tctttccgta ccctggaaag cggtcgtgcc
    19441 gttgccttta tgatggatga tgctctgctg gccggtgaac gtgcgaaagc gaagaaacca
    19501 gacaactggg atatcgtcgg caagccgcag tctcaggaag cctatggttg catgttgcgt
    19561 aaagacgatc cgcagttcaa aaagctgatg gatgacacta tcgctcaggt gcagacctcc
    19621 ggtgaagcgg aaaaatggtt tgataaatgg ttcaaaaatc ccattccgcc gaaaaacctg
    19681 aacatgaatt tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc gaatgacaaa
    19741 gcactgaact aattacaaga accaggggcg gaaattctag ccctctcgat tgttacgtag
    19801 cacggacaga ctatacgcct gatggtcgtt ccccatcggg cctgaaaacc gcaatacgct
    19861 gggtaacaat cttcgagggt agcagttaac gctgctaccc tcttttttct ggagtagatt
    19921 tatgtctata gactggaact ggggtatttt tttacaacaa gccccgttcg gcaacaccac
    19981 ctatcttggt tggatctgga gtggttttca ggtgacgatc gctttgtcga tctgcgcctg
    20041 gattatcgct ttcctcgtcg gttcattttt cggtatttta cgtaccgttc caaaccgttt
    20101 tctctccggt ctgggtacgt tgtatgtcga actgttccgc aacgtgccgt tgattgtgca
    20161 attctttacc tggtatctgg tgatcccgga gctgctgccg gagaaaatcg gcatgtggtt
    20221 taaggccgag ctggatccca atattcagtt tttcctttca tccatgctct gcctggggct
    20281 gtttaccgcc gcccgtgttt gcgaacaggt acgcgccgcg attcagtcgc tgccgcgtgg
    20341 gcagaagaat gccgcgctgg ctatgggtct gacgctgccg caggcttacc gctacgtact
    20401 gctgcctaat gcttatcgcg ttatcgtccc gccgatgacc tcagagatga tgaacctggt
    20461 gaaaaactcc gccatcgcct cgaccatcgg tctggtagat atggcagcgc aggcgagtaa
    20521 attgctcgat tactcggctc acgcctggga gtcgtttacc gccattacgc tagcgtatgt
    20581 gtttattaac gcttttatca tgctggtgat gacgctggtt gaacgtaaag ttcgcctgcc
    20641 tggcaacatg gggggcaaat aatgtacgag tttgactgga gttccattgt cccttccctg
    20701 ccatatctgc tcgacgggct ggtgatcacg ctgaaaatca ccgtcacggc agtcgtgatt
    20761 ggtattttgt ggggcaccat gctggcggtg atgcgcttgt ccagctttgc gcccgtagcg
    20821 tggtttgcca aagcctacgt taacgtattc cgctcgattc ctttagttat ggttttgctg
    20881 tggttttacc tgatcgtgcc gggttttctg caaaacgtgc tgggattatc accaaaaaat
    20941 gatatccgtt taatttcggc gatggtggcg ttttcgatgt ttgaagcggc ctactattca
    21001 gagattatcc gtgccggtat tcaaagtatt tctcgtggtc agtcgagcgc cgcgctggcg
    21061 ttgggaatga ctcactggca gtcgatgaaa ctgattattc tgccgcaggc gttccgcgcg
    21121 atggtgccgc tgctgctcac tcagggcatc gtactgttcc aggatacctc gctggtgtat
    21181 gtgttatctc tggccgattt cttccgtacc gcctcaacca ttggtgagcg tgatggcacg
    21241 caggttgaga tgatcctgtt tgccggattt gtttatttcg ttattagcct tagcgcgtcg
    21301 ttgttggtca gctacttgaa aagaaggaca gcataatgat taccctgaaa aatgtttcaa
    21361 aatggtatgg tcactttcag gtgctgaccg actgctcaac cgaagtgaaa aaaggcgaag
    21421 tggtggtggt ttgcggcccg tctggttccg gcaaatcaac gctgattaaa accgtcaacg
    21481 gcctcgaacc agtgcagcaa ggtgaaatca ccgtcgatgg tatcgtggtt aacgacaaga
    21541 aaaccgatct ggcaaagctg cgttctcgcg tcgggatggt attccaacat ttcgagctgt
    21601 tccctcatct gtcgattatc gaaaacctga ccctggcgca ggtgaaagtg cttaaacgcg
    21661 ataaagcacc ggctcgtgaa aaagcattga aattgctgga gcgcgtggga ctttccgcac
    21721 atgccaataa atttccggcg cagctttccg gcggccagca gcagcgtgtg gcaattgctc
    21781 gcgcattgtg tatggatcct attgcgatgc tgttcgacga acccacatcg gcactagacc
    21841 cggagatgat caacgaagta ctggacgtga tggtggaact ggcgaacgag gggatgacca
    21901 tgatggtggt gacccacgaa atgggctttg cccgtaaagt ggcgaatcgg gtgatcttta
    21961 tggatgaggg taaaattgtt gaagactcgc cgaaagatgc gttcttcgat gatccgaaat
    22021 cggaccgcgc aaaagacttc ctcgcgaaaa tcctgcatta atcactctgg cgcgcatacc
    22081 caagtgcgcg ccatctcacg ctttatcgtc tcgcccctcg tatctgttac atcctggcgt
    22141 tacccttgtc gcaaagaagc acacaacaag gagcaacaat ggcactgcca attctgttag
    22201 attgcgaccc aggtcatgat gacgctatcg caatagttct cgccctcgcc tcaccagagc
    22261 ttgatgtcaa agcaattacg tcttccgccg gaaaccagac accagaaaaa accttacgca
    22321 atgttctgcg tatgctgacc ttgcttaatc gcaccgatat tccggtggca ggcggcgcgg
    22381 taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt gcacggcgaa agcggtctcg
    22441 acggcccggc attaccggaa ccgacgttcg caccgcaaaa ctgtacggcg gtagagctga
    22501 tggcgaaaac gctgcgtgaa agtgcggaac ctgtcacgat tgtgtctacc ggaccgcaaa
    22561 ctaacgttgc cttgctgctc aatagccatc cggaactgca tagcaaaatt gcccgtatcg
    22621 tgattatggg tggcgctatg ggactgggta actggacgcc tgcggctgaa tttaatattt
    22681 acgttgaccc ggaagcggca gaaattgtct tccagtcagg gatcccggtg gtgatggccg
    22741 gtctggatgt tactcacaaa gcacaaatcc acgttgaaga cactgagcgt ttccgcgcga
    22801 ttggcaaccc tgtttcaacc attgttgccg aactgctgga tttcttcctc gaatatcata
    22861 aagacgaaaa atggggcttt gtcggcgcac cgctacatga cccatgcacc atcgcctggc
    22921 tgttaaaacc ggagttgttt accactgttg agcgctgggt tggcgtggaa acacagggga
    22981 aatataccca gggtatgacg gttgttgatt attattatct gactggcaat aaaccgaatg
    23041 ccactgtaat ggtcgatgtt gatcgtcagg gctttgttga tttactggct gatcgtctga
    23101 aattttacgc ttaacacacg tcattgatta agatcaccac gcgttacctg ggtggtctta
    23161 atctgtttat ctctttttct ttcaagatat aaatgcaatg gataatgcag aactcgccat
    23221 tggtatcgat ctcggtacta ccaatagttt aattgccgtc tggaaagacg gtgccgcgca
    23281 attaattcca aataagttcg gtgaatattt aacaccatcc ataattagca tggatgaaaa
    23341 taatcatatt ttagtcggaa aaccggctgt atcacggcgt acttcgcatc cggataaaac
    23401 ggcagcgtta tttaaacgtg caatgggcag taataccaac tggcggttag gcagcgacac
    23461 atttaacgcg ccagaactgt cctctttggt attacgctca ttaaaagaag atgccgaaga
    23521 atttctgcaa cgtccgatta aagatgtggt gatctctgtt ccggcttatt tcagcgatga
    23581 acaacgcaag catacccgtt tagcagcgga gttagccggt ttaaatgcgg tacgcttaat
    23641 taatgaaccc acagcagctg cgatggcgta tggcctgcat acccaacaaa atacccgttc
    23701 gctggtgttt gatctcggtg gcggcacgtt tgacgtcacg gtgcttgagt acgccacgcc
    23761 ggtgattgaa gttcacgcct ccgctggcga caacttcctc ggtggcgaag attttaccca
    23821 tatgctggtc gatgaggttt taaaacgcgc ggatgtcgcc aggaccacgc ttaacgagag
    23881 tgaactggca gccttgtatg cctgtgtgga agcggcaaaa tgtagcaatc aatcgccaat
    23941 acacattcgc tggcagtacc aggatgaaac gcgggaatgc gaattttacg agaacgaact
    24001 ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg ccgattgaac aggcgttgcg
    24061 cgatgcgcgt ctgaagccga gtcaaatcga cagtctggtg ctggttggcg gcgcgtcaca
    24121 aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc aaattaccgt atcaaagtta
    24181 cgatccgagc accattgtcg cgctgggcgc agcaatccag gcagcctgcc gcttacgcag
    24241 tgaagatatt gaagaagtga tcctcactga tatttgccct tactcgttgg gcgttgaggt
    24301 taaccgccag ggcgtttccg gcattttctc gccgattatt gaacgaaaca ccactgtgcc
    24361 cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa caggattcta ttacggttaa
    24421 cgtctatcag ggagaaaacc acaaagttaa aaacaacatt ctggtggaat ccttcgatgt
    24481 gccgttgaag agaaccgggg cttatcagtc gattgatatt cgctttagtt atgatatcaa
    24541 cggtttgctt gaagttgacg tgcttctgga agacggcagc gttaagtcca gagtgattaa
    24601 ccacagcccg gtaacattga gcgcgcagca gattgaagag agtcggacgc ggttatccgc
    24661 attgaaaatt tatccgcgcg atatgctcat caaccgcacc tttaaggcca aactggaaga
    24721 gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt ggccgggtga tcaccgattt
    24781 tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat gaggtcaggc ggcaggcgag
    24841 cgtttattta gccattgaga tcccataatc acggctgccg gatgcggcgt aaatgctgca
    24901 tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac atcgcgattt tgccgagagc
    24961 ataacctatc accagcaccg ttaagctgat tttgataatg ctggtaatgc tctgtggttt
    25021 ttcatcctca tcattttttt cttcatggtt ttcaggtgtt ggctcatcga catcttcatc
    25081 ctccggctcg agttttactt tcgtcaacag catccgggtg ataatggcct ctaatggatg
    25141 agcatctccg ccttccagcc ttgtattaag acgggccagc atctcttgtt tcggaaacgc
    25201 ttcaagtact acctgttccg ctacccgata caaatccagt aacggtgaat cttcttctgt
    25261 ctcgctaccg actctcgatt tccaccacgc ccatgctttg cgtttgaaga atagcgactc
    25321 aataaacgat tgacctaacg gcgatagttt tccgctatgt aaaatttccg cccataacag
    25381 acgcgtccgg gcgctctcac caggaacata tttcaacggt ttaccttgcg catctaccat
    25441 gctccgcgct tcgataacca tcactaataa cgcgaaataa tccgggcaat gttgtgcgct
    25501 ccactgcaac aacaaattat cggcttgtgt agatgggtaa cttccccagt acgcgcagag
    25561 atcggtaagc aggctttccc cctcgccgca gtacaaacgt tgtgcacact ggtaataccg
    25621 ggcatcttcg ctttctggtt cttccatttg ccactgttcg gctacaggaa tgagttccgc
    25681 catacctgtt tgcaccgagc tgtaccagcg caacagtttg cgatgtaact ttgcatcatc
    25741 aggaatgaac cacggacctt ccatcgccaa aaacgcagtg acatagtcag gatggtaacg
    25801 gcagcaacgt tccagcgcga agtaatactc aacagtctgg ttctgcaacg caacgggata
    25861 taccagaagg ccaaggaaat cgaaacaatc accagcctga atggcctcaa gaaaagcctc
    25921 cctttcttac tcatctattc cttcgctgtc attaaacgaa tgccagttca aacgctctgc
    25981 cagcaaagaa gcacaattta atgaaagata tcgcgcttct atagcgattg cacacagcgg
    26041 ccagcgtaat tgatcgacat cctccatgtt ccaggtattt aattgctgaa taaatttctg
    26101 ccaggcggag ggctgaaaac gatcactttc tgaatccagt aatgccctga atacacgtag
    26161 tatttcatgc tcagtggcat catctttttc ttcatcatct gcttcctcga caggatttac
    26221 cgccagtcgc agggcctctt cataggcctg gcgtaatgct ttaaaccctt gcggatcggt
    26281 ttcgggatga cataacggta agcgagcaag ataagcctgg cggataatgt ctatttgcgt
    26341 cgtgctttca atttcgagga tttgccaaca tgttttcata ggtcgctcac atccttaagc
    26401 gttaccgggc attcaggctg gacagttaat ggctgccagg gaagcgtggc atagaaacga
    26461 tcgcttcccg cagccagctc ttcgaacatc cgactcccgg cgctatcgta tttgtacagg
    26521 gttagcgcct cgcgtagcga agcgttgtct tcgcattgag aagattgcca gtagagacgc
    26581 cctagtgaat aggcggaaaa atagtgcatc cagctgtgat agtaatggtg agctcgttca
    26641 tagacgcgcg attgcagcca taaactctcg tcttccgtca gccacttgtt gagcacaccg
    26701 acccgtgaca gaaaccccat tcgaacataa tcccaagctt tgattccccc ttccccacaa
    26761 cacatagcgg tttgggcaac gaattgggta taaatcaacc ctctctccga gccaccagca
    26821 attaatgctt tccactcttc aggggaagag cgatgccatg tcagatataa gccagctaaa
    26881 tcattggcat gaccatcatc agtcatctga taaatagtct gaattaaatc atcacgatta
    26941 tttattcccc aggatgatga gagatcgaat tcttttatcc cggtatacat ttttggcgag
    27001 tcatgcgcgg gtttgcaacc ctgattaagt atttccatgg gcgcagaaag cgcaaaaaga
    27061 agatattgtt cttccttatt catttgttaa taatccctta agtgcat
//
LOCUS       DAZFKY010000052        26309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFKY010000052 DAZFKY010000000
VERSION     DAZFKY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            274..1896
                     /gene="pckA"
                     /locus_tag="ACHKCL_001905"
     CDS             274..1896
                     /gene="pckA"
                     /locus_tag="ACHKCL_001905"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5762876.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(1972..3324)
                     /gene="envZ"
                     /locus_tag="ACHKCL_001906"
     CDS             complement(1972..3324)
                     /gene="envZ"
                     /locus_tag="ACHKCL_001906"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5762877.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(3321..4040)
                     /gene="ompR"
                     /locus_tag="ACHKCL_001907"
     CDS             complement(3321..4040)
                     /gene="ompR"
                     /locus_tag="ACHKCL_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5762878.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4268..4744
                     /gene="greB"
                     /locus_tag="ACHKCL_001908"
     CDS             4268..4744
                     /gene="greB"
                     /locus_tag="ACHKCL_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5762879.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            4841..7162
                     /gene="yhgF"
                     /locus_tag="ACHKCL_001909"
     CDS             4841..7162
                     /gene="yhgF"
                     /locus_tag="ACHKCL_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5762880.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            7600..7827
                     /gene="feoA"
                     /locus_tag="ACHKCL_001910"
     CDS             7600..7827
                     /gene="feoA"
                     /locus_tag="ACHKCL_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5762881.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            7844..10165
                     /gene="feoB"
                     /locus_tag="ACHKCL_001911"
     CDS             7844..10165
                     /gene="feoB"
                     /locus_tag="ACHKCL_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5762882.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            10165..10401
                     /gene="feoC"
                     /locus_tag="ACHKCL_001912"
     CDS             10165..10401
                     /gene="feoC"
                     /locus_tag="ACHKCL_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5762883.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            10604..11482
                     /gene="rpnA"
                     /locus_tag="ACHKCL_001913"
     CDS             10604..11482
                     /gene="rpnA"
                     /locus_tag="ACHKCL_001913"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO5762884.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(11509..12279)
                     /gene="bioH"
                     /locus_tag="ACHKCL_001914"
     CDS             complement(11509..12279)
                     /gene="bioH"
                     /locus_tag="ACHKCL_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5762885.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            12317..13000
                     /gene="gntX"
                     /locus_tag="ACHKCL_001915"
     CDS             12317..13000
                     /gene="gntX"
                     /locus_tag="ACHKCL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5762886.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13059..13634
                     /gene="nfuA"
                     /locus_tag="ACHKCL_001916"
     CDS             13059..13634
                     /gene="nfuA"
                     /locus_tag="ACHKCL_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5762887.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            13995..15311
                     /gene="gntT"
                     /locus_tag="ACHKCL_001917"
     CDS             13995..15311
                     /gene="gntT"
                     /locus_tag="ACHKCL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5762888.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(15356..17440)
                     /gene="malQ"
                     /locus_tag="ACHKCL_001918"
     CDS             complement(15356..17440)
                     /gene="malQ"
                     /locus_tag="ACHKCL_001918"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5762889.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGRYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(17450..19843)
                     /gene="malP"
                     /locus_tag="ACHKCL_001919"
     CDS             complement(17450..19843)
                     /gene="malP"
                     /locus_tag="ACHKCL_001919"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5762890.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            20455..23160
                     /gene="malT"
                     /locus_tag="ACHKCL_001920"
     CDS             20455..23160
                     /gene="malT"
                     /locus_tag="ACHKCL_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5762891.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            23227..23505
                     /locus_tag="ACHKCL_001921"
     CDS             23227..23505
                     /locus_tag="ACHKCL_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5762892.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            23496..23981
                     /locus_tag="ACHKCL_001922"
     CDS             23496..23981
                     /locus_tag="ACHKCL_001922"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5762893.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(24031..25059)
                     /gene="rtcA"
                     /locus_tag="ACHKCL_001923"
     CDS             complement(24031..25059)
                     /gene="rtcA"
                     /locus_tag="ACHKCL_001923"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5762894.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(25063..26289)
                     /gene="rtcB"
                     /locus_tag="ACHKCL_001924"
     CDS             complement(25063..26289)
                     /gene="rtcB"
                     /locus_tag="ACHKCL_001924"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5762895.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         6207 a         7233 c         6640 g         6229 t
ORIGIN      
        1 tttaccggtt gaatttgcat caatttcatt caggaatgcg attccactca caatattccc
       61 gccatataaa ccaagattta accttttgag aacattttcc acacctaaaa tgctatttct
      121 gcgataatag caaccgtttc gtgacaggaa tcacggagtt ttttgtcaaa tatgaatttc
      181 tccagatacg taaatctatg agccttgtcg cggttaacac ccccaaaaag actttactat
      241 tcaggcaata catattggct aaggagcagt gaaatgcgcg ttaacaatgg tttgaccccg
      301 caagaactcg aggcttatgg tatcagtgac gtacatgata tcgtttacaa cccaagctac
      361 gacctgctgt atcaggaaga gctcgatccg agcctgacag gttatgagcg cggggtgtta
      421 actaatctgg gtgccgttgc cgtcgatacc gggatcttca ccggtcgttc accaaaagat
      481 aagtatatcg tccgtgacga taccactcgc gatactttct ggtgggcaga caaaggcaaa
      541 ggtaagaacg ataacaaacc tctctctccg gaaacctggc aacatctgaa aggcctggtg
      601 accaaacagc tttccggcaa acgtctgttc gttgtcgacg ccttctgtgg tgcgaacccg
      661 gatactcgtc tttccgtccg tttcatcacc gaagtggcct ggcaggcgca ttttgtcaaa
      721 aacatgttta ttcgcccgag cgatgaagag ttggcaggtt tcaaaccaga ctttatcgtt
      781 atgaacggcg cgaagtgcac taacccgcag tggaaagaac agggtctgaa ttccgaaaac
      841 ttcgtggctt ttaacctgac cgagcgcatg cagctgattg gcggcacctg gtatggcggc
      901 gaaatgaaga aagggatgtt ctcgatgatg aactacctgc tgccgctgaa aggtatcgct
      961 tctatgcact gctccgccaa cgttagcgag aaaggcgatg ttgcggtgtt cttcggcctt
     1021 tccggcaccg gtaaaaccac cctttccacc gacccgaaac gtcgcctgat tggcgatgac
     1081 gaacacggct gggacgatga cggcgtgttt aacttcgaag gcggctgcta cgcaaaaact
     1141 atcaagctgt cgaaagaagc ggaacctgaa atctacaacg ctatccgtcg cgatgcgctg
     1201 ctggaaaacg tcaccgtgcg tgaagatggc actatcgact ttgatgatgg ttcaaaaacc
     1261 gagaacaccc gcgtttctta tccgatctat cacatcgata acatcgttaa gccggtttca
     1321 aaagcgggtc acgcaacgaa agttatcttc ctgaccgcag acgcctttgg tgtgctgcca
     1381 ccggtttctc gtctgactgc cgatcaaacc cagtaccact tcctctctgg cttcaccgcc
     1441 aaactggccg gtactgagcg tggcatcacc gaaccgacgc caaccttctc cgcttgcttc
     1501 ggcgcggcat tcctgtcgct gcacccgact cagtacgcag aagtgctggt gaaacgtatg
     1561 caggcggcgg gcgcgcaggc ttatctggtt aacactggct ggaacggcac tggcaaacgt
     1621 atctcgatta aagatacccg cgccattatc gacgccatcc tcaacggttc gctggataat
     1681 gcagaaacct tcactctgcc gatgtttaac ctggcgatcc caaccgaact gccgggcgta
     1741 gacacgaaga ttctcgatcc gcgtaacacc tacgcttccc cggaacagtg gcaggaaaaa
     1801 gccgaaaccc tggcgaaact gtttatcgac aactttgata aatacaccga cacccctgcg
     1861 ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat ttgaagctgg agaatatcta
     1921 tccagtatct tatagaaagc aaaacgggag gcaccttcgc ctcccgttta tttacccttc
     1981 ttttgtcgtg ctctgcgccc gcgttaccgg cacaggcagc caggcgcgaa tggaaagccc
     2041 gccccgctcg ctggtgccaa gctccagcat cccgttatgg ttatccacga tacgctgcac
     2101 aatcgccagc cctaatcccg tgccgctaat ggtgcgtgca ctgtcgccgc gaacaaacgg
     2161 ctggaaaagg tgcttacgtt gttccggcgc aattcccgga ccgtcatctt ccacctggaa
     2221 ccaggcgcga ttcggctccg tcccgctgct gactttgatc cagccattgc cgtaacgggc
     2281 ggcgttgacc accatattcg ccaccgcgcg tttgatcgac agcgggtgca ttttcacttc
     2341 aatgctgccg gggtaaagcg cggtttcaat ttcccgctca tagccacttt cggcagcaat
     2401 aacctcaccg agtactgcgt tgagatccgc catttccatc ggcatctcct gtccggtgcg
     2461 caggtagtcg ataaactgct caatgatggc attgcactcc tcgatatctt tattgatcga
     2521 ttctgccagg tagccatcct gctcgctcat catctcggtc gccaggcgaa tacgcgtcag
     2581 cggtgtacgc aagtcgtgac ttacccccgc catcagcagc gtgcggtcat ccgccagttg
     2641 cttaacacca gccgccatat ggttaaaggc gcgggtaacg gaacgcacct cggacgcgcc
     2701 atactcacgc agcggcggcg gaataatccc tttaccaacc tgcaaggctg cgtgttcgag
     2761 atcgaccaac ggtcgattct ggatacgaat aaacagccac gccccgccta tcgccaatag
     2821 cataatcgcc aacgtatagc ggaacagcgg agagaaatcg ccctgatgaa tttcggtcag
     2881 cggcacgcgt acccagatat tgggcgacag ccaggttttc agccagacga caggcgaact
     2941 tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt tgctgtgcca tctgatggct
     3001 taagaattca tagtgttgcg cccaacgcag acctgcctct tcggcagcct cgttggagta
     3061 gagagagatc cccagctcac ggtagatctc ccgacggaaa gcgggaggca caaccaactg
     3121 cgtgccgtcc tccagttgca gtttgtcggt catcaacata cgcacttcgt acgcgaggac
     3181 tttattaaac tgctggaggc tcggcaaaat cgcgaagttc agcaccacca gataagtcgt
     3241 caccaggctg gcgaacagca aggtgacgat gagcaataac gtacgggcaa atgaacttcg
     3301 tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg gtacaaagac gtagcccagg
     3361 ccccaaacgg tctgaatgta acgcggatgc gctggatctt cttccaccat gcggcgcaga
     3421 cgagaaatct gcacgtcgat ggagcgttcc attgcggaat attcacgacc acgggcaagg
     3481 ttcatcagct tatcgcggga gagcggctca cgcggatggc tgaccagtgc cttcagtacc
     3541 gcaaactcac cgctggtgag cggcatcggc tcgtcttcgc ggaacatttc gcgcgtaccg
     3601 aggttaagtt tgaacttacc gaaagcaatt accgcctctt cctgtgacgg tgcgcctggt
     3661 agctcgttcg cctgacgacg cagcaccgca cggatacggg ccagcagttc acgcgggtta
     3721 aacggttttg gaatgtaatc gtcagcgcca atctccaggc caacgatacg gtccacttct
     3781 tcccctttcg ccgtcaccat aatgatcggc atcgggttgc tctgactacg aagacgtcgg
     3841 caaatcgaca agccatcttc accaggtaac attaaatcca gtaccataag atggaaagat
     3901 tcacgagtca gcaggcgatc catctgttct gcattagcga cgcttcgaac ctggaagcct
     3961 tgttcggtga gataacgttc cagcagcgca cgcaggcgca tgtcgtcatc gaccaccaga
     4021 atcttgtagt tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg
     4081 tattcttaaa aaagctcacg ttcgtcacca gctaaatctg gtatgaattt cagcctaaat
     4141 tgttacaaag catattaaac agcagcttaa gtatacaatt tattcggcga aacattattg
     4201 attctgttga tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga caaagggaat
     4261 caacgagatg aaaacgcccc tggttacccg ggaagggtat gaaaaactca aacaagagct
     4321 taattatctc tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag
     4381 tctgggcgac cgcagcgaaa atgctgacta tcagtataat aaaaagcgcc tgcgtgaaat
     4441 cgaccgtcgc gtgcgctatc tcactaaatg catggaaaat ctcaaaatcg tcgattactc
     4501 cccgcagcag gaaggcaaag tcttttttgg cgcgtgggtg gagattgaaa acgacgatgg
     4561 cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt tttggccgta aagattacat
     4621 ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa gaagtcggcg atctggctgt
     4681 ggtgaatact cctgccgggg aagcgagctg gtatgttaat gctatcgagt acgtgaaacc
     4741 gtaaggaaga gtcttaacct cctgccgatg gctggcattt ttgccagcca gtccgtataa
     4801 ctatcccctg atttttgatc cgaaaagatg aactcaaacc atgatgaatg attcgttctg
     4861 ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct
     4921 gcttgacgaa gggaataccg tgccttttat tgcacgttat cgtaaggaaa tcaccggcgg
     4981 tctggatgac acgcagctgc gtaatctgga aacgcgtctg ggatacctgc gcgagctgga
     5041 agagagacgt caggcgatcc tcaagtccat ttccgagcaa ggcaaactca ccgatgatct
     5101 ggcgaatgcc atcaacgcca ccctaagcaa aaccgaactc gaagacctct acctgcccta
     5161 caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg agccgttggc
     5221 tgacctgctg tggagcgatc cgtcacacac gccagaagtc gccgctgcgc aatatattga
     5281 tgccgataaa ggcgtggcag ataccaaagc cgcgctggac ggcgcgcgct atatcctgat
     5341 ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg cgtgattatc tgtggaagaa
     5401 cgcgcatttg gtttctacgg tggtgagcgg taaagaagag gaaggggcga aattccgcga
     5461 ctatttcgat catcacgaac cgttgtccac ggtgccttct caccgcgcgc tggcgatgtt
     5521 ccgtgggcgt aacgaaggcg tacttcagct ttcgctgaat gccgatccac agttcgatga
     5581 gccgcccaaa gagagctatt gcgagcaaat catcatggat caccttggcc tgcgcctgaa
     5641 caatgccccg gcggatagct ggcgcaaagg cgtggtgagc tggacgtggc gcatcaaggt
     5701 gctgatgcat ctggaaaccg aactgatggg caccgtgcgc gaacgtgcgg aaaatgaagc
     5761 aatcaacgtc tttgcccgta acctgcacga tctgctgatg gctgcccctg ccggactgcg
     5821 tgcaacgatg ggcctcgatc cgggtctgcg taccggggta aaagtggcgg tggtcgatgc
     5881 cactggcaaa ctggtagcga ccgataccat ttacccgcac accggacaag ccgcaaaagc
     5941 agcgatgact gttgctgcgc tgtgtgaaaa gcataacgtt gaactggtgg cgatcggcaa
     6001 cggtacagct tcccgcgaaa ccgaacgttt ctatctcgac gtgcagaagc agttcccgaa
     6061 agtgaccgca cagaaagtga tcgtcagcga agctggcgcg tcggtttact cggcttccga
     6121 gctggcagcg caggagttcc cggatctcga cgtttcgctg cgtggcgcgg tttctatcgc
     6181 ccgccgtttg caggatccgc tggcggagct ggtgaaaatc gatccgaaat ctatcggcgt
     6241 aggtcagtat cagcatgacg tcagccagac gcaactggcc cgcaaactgg atgcagtagt
     6301 agaagactgc gtaaacgccg ttggcgtcga tctcaacacc gcttctgttc cgctattaac
     6361 tcgcgtggcg ggcctgacgc gaatgatggc gcaaaacatc gtggcctggc gcgatgagaa
     6421 cggccagttc cagaaccgtc agcaactgct gaaagtcagc cgtctggggc cgaaagcctt
     6481 cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgac aacccgctgg acgcctctac
     6541 cgttcacccg gaagcctatc cggtggtgga acgcattctg gcagcaacac agcaggcact
     6601 gaaagatctg atgggtaaca gcagcgaact gcgtaacctg aaagcgtctg actttactga
     6661 tgaaaaattc ggtgtgccga cagtaactga catcatcaaa gagctggaaa aaccgggtcg
     6721 cgatccgcgt ccggaattta aaaccgctca gtttgccgat ggcgtcgaga caatgaacga
     6781 cctgcaaccg ggtatgatcc tcgaaggcgc tgtcaccaac gtcaccaact ttggcgcgtt
     6841 tgtcgatatc ggcgtgcatc aggacggcct ggttcacatc tcttcattgt cgaacaagtt
     6901 tgtggaagat ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga aagtgctgga
     6961 agtggatctt cagcgtaaac gtatcgccct gactatgcgt ctggacgagc agcctggcga
     7021 aaccaactct cgtcgcggcg gcggtaacga tcgcccgcaa aacaaccgcc cggcagccaa
     7081 accacgcggt cgtgaagcgc agcctgccgg taacagcgcg atgatggacg cgctggcggc
     7141 agcaatgggc aaaaaacgtt aaacgcccgg actggcccct aaacctgcgc gataaggcgt
     7201 tcacgccgca tctggcaacg aacgccttgc ctgacataaa agtgccggag aatatctccg
     7261 gcatatttat tccacagcca aactcacaat atatttcgtt aatatttatc acttcattaa
     7321 caactgaaac cttaattaaa cattagccag tcctggtaat tcgctattcg aattatattt
     7381 tcgctgcgat ataaccttga gccacatcaa cattgagtca gattattatt caaaccaaca
     7441 ttcgcacaca ttttaagtat tgctgataga aaccattctc attatcattg tactattgat
     7501 tatttaatct ctccttcgtt ggcaaatcat ctggtctcat gtcgctgtca aacgccccat
     7561 gaggtagtta tccagttaat gagaaacaag taggcaccta tgcaatacac tccagatact
     7621 gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg catatcgcca aaaactgctt
     7681 tctcttggca tgttacctgg ctcctctttt aatgtggtgc gcgtcgctcc actcggcgac
     7741 cccattcata tcgaaacccg tcgtgtgagc ctggtattac gcaaaaaaga tctggcctta
     7801 ttagaagtgg aagcggtttc ctgttaatac ggtgataaca acaatgaaaa aattaaccat
     7861 aggcttaatt ggtaatccaa attctggcaa gacaacctta tttaaccagc tcactggcgc
     7921 acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc
     7981 caccaccgat catcaggtca cgctggtgga cctgcccggc acctattctc tgaccaccat
     8041 ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac tacattttga gtggcgacgc
     8101 cgacctgctg attaacgtgg tggatgcgtc taaccttgag cgtaacctgt acctgacgct
     8161 acaactgctg gaactcggca ttccctgcat cgtggcactg aacatgctcg acattgccga
     8221 gaagcaaaat attcgtattg aaattgatgc tctgtcggcg cgtcttggct gcccagtgat
     8281 cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta
     8341 taaagctaac gagaatgtgg aactggtgca ttacgcacag ccgctactca acgaagcaga
     8401 ttcactggca aaagtgatgc cttccgacat cccgctgaaa caacgtcgct ggctgggcct
     8461 gcaaatgctg gaaggcgata tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct
     8521 tgatgccgcc ctcgcccgtc tgcgtaatga gatggacgat ccggcgctgc acattgccga
     8581 tgcgcgttac cagtgcattg ctgccatctg tgatgtggta agcaacaccc tgacggcaga
     8641 acccagccgt ttcaccactg cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc
     8701 gattttcctc tttgtgatgt acctgatgtt cctgctggct atcaacatcg gcggggcgtt
     8761 acagccgctg tttgacgtcg gctccgtggc tctatttgtg catggtattc aatggattgg
     8821 ctacacgctc cacttcccgg actggctgac tatcttcctc gcccagggcc tgggtggcgg
     8881 tattaacacc gtgctgccac tggtgccgca gatcggcatg atgtacctgt tcctctcctt
     8941 ccttgaggac tccggctata tggcgcgtgc ggcgtttgtg atggaccgcc tgatgcaggc
     9001 gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc ggtttcggtt gtaacgtacc
     9061 gtcggtaatg ggtgcacgta cgcttgatgc accgcgtgaa cgtctgatga ccatcatgat
     9121 ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc gcagtattcg cggctgcctt
     9181 cttcgggcag aacggtgcgc tggcggtctt ctcgctgtat atgctcggta tcgtgatggc
     9241 ggtgctgact ggcctgatgc tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt
     9301 catggagctg ccggtctacc atgtaccaca cgttaaaagc ctgattatcc agacctggca
     9361 gcgtctgaaa ggcttcgttc tgcgtgctgg taaagtgatt atcatcgtca gcattttcct
     9421 gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc gataacatca acgactcggc
     9481 gctggcttcc gtcagccggg tgatcacccc ggtcttcaag ccgattggcg tgcatgaaga
     9541 taactggcag gcaacagttg gcctgtttac gggtgcaatg gcgaaagaag tggtggtggg
     9601 tacgctcaac accctctaca ccgcagaaaa tattcaggac gaagagttca atccggcgga
     9661 atttaacctc ggtgaagagt tgttcagcgc ggtagatgaa acctggcaga gcctgaaaga
     9721 caccttcagc cttagcgtac tgatgaaccc cattgaagct agcaaaggcg acggcgaaat
     9781 gggtaccggg gcgatgggcg tgatggatca gaaattcggt agcgcagctg ccgcatacag
     9841 ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg gtgatgggag ccatcgcccg
     9901 tgaatcaagc cgtggctgga tgggcttctc catcctgtgg gggctgaata tcgcttactc
     9961 actggcaaca ttgttctatc aagtcgccag ctacagtcag cacccaactt acagcctggt
    10021 gtgcattctg gcggttatcc tgtttaacat cgtggttatc ggtctgctgc gccgcgcgcg
    10081 tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg gtaagtagtt gctgcgcagc
    10141 cagcaccacc ggtgattgcc attaatggct tcacttattc aggtgcgtga tttgctggcg
    10201 ttacggggcc gtatggaagc ggcccagata agccagacat tgaacactcc acagccaatg
    10261 attaacgcca tgctgcaaca actggaaagt atgggcaaag ccgtgcggat tcaggaagaa
    10321 cctgacggct gcctctccgg cagttgtaaa agctgcccgg aaggaaaagc ctgtctgcgc
    10381 gagtggtggg cgctgcgtta accatactcc atcgcctgtt ttgaaaagcc cggtatgcgt
    10441 ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat gcaattcttg cagcgcgacg
    10501 taagttcctc tgaaagcgtc tcgcagggat gaaaactggc taatgcacag gtgtggagtg
    10561 gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac gccatgagca aaaagcagag
    10621 ttccacccca cacgatgcgc tgttcaaact ctttttacgc caaccggaga cggcacgcga
    10681 ttttcttgcg tttcatttac cagcacccat tcacgcgctc tgtgatatga aaaccctcaa
    10741 gctggagtcg agcagcttta ttgatgacga tctgcgtgaa agctattccg atgtgctgtg
    10801 gtcggtgaaa acagaacaag gaccaggata catctattgt ctgattgaac atcaaagcac
    10861 ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca
    10921 ccttgatgcc ggatacaaaa cgttgccgat ggtggtgcca ttgttgtttt accacggtat
    10981 tgaaagcccc tatccctatt cgctgtgttg gctggattgt ttcgccgatc ccaaactggc
    11041 aaggcagctt tatgcctccg catttccgct gattgatatc accgtcatgc ctgatgatga
    11101 aatcatgcag caccgacgca tgacgctgct ggagttaatt caaaaacata ttcgtcaacg
    11161 cgacctgatg gggctggtag agcaaatggc ctgcttatta agtagtggat acgctaatga
    11221 cagacaaatc aaagggctgt ttaattacat actgcaaacc ggcgatgcgg tacgttttaa
    11281 cgattttatc gacggcgttg ccgaacgatc accgaaacac aaggagagtt taatgactat
    11341 tgcggaaaga ttgcggcagg agggggaaca atccaaagcc ctgcatatag ccaaaataat
    11401 gcttgaatcc ggagtccctc ttgcagacat catgcgcttt accgggctgt cagaagaaga
    11461 gttggctgcg gcgagtcagt aaagttctgt ctcgccattc aaaagtgcct acaccctctg
    11521 cttcaacgcc accagcacgt gacgaaactc gaccggatgc gaaataaacg gcgcatgagc
    11581 cgctttggcg aagatgtatg actcgctgtg tggccagagt ttatccagca tcggcaccac
    11641 tttgcgcggc accagaccat cgagatagcc atacagtcgc aaaaacggca tgggtacgtt
    11701 ttgcagcggc aagcggagat caaccgtttt gaggatttcc agcccaccat tgagcacgtc
    11761 aacctccggc atcggtaacg ccagaacggt tttcttcaac gcccgcgcat cctgacgcgc
    11821 cgtttcagta cccatggttt gtaacgcaag gaaccgttcc accgtacgct gaaaatcatc
    11881 actgagttgc tgctgaaatc ccgccagcac gtccggtttt atccccggcc actcgtcacg
    11941 agcactaaaa caaggtgacg acgccacggt gaccagcgcc tgaacacgct cgggatgggt
    12001 taacgcaatc tggcttgcca ccagcccgcc cagactccag cctaaccaaa tggctttatc
    12061 aggtgcctgt cgcagcacgg cttcggccat ctccgcaagt gacagcgcac caaatccccg
    12121 gctacgcccg aagccgggca ggtcaacaag atgcagcgta aaatgcgagc taagttcctc
    12181 gtcaatgcaa cgccacactt cagcattcag tccccatccg tgcagcagca caagatgaac
    12241 attcccctga cctttggtct gccaccagat gttattcatc cgctattgtt ctcttttgac
    12301 ttacaaggat gaacatatgc taacagtacc gggattatgc tggctatgcc gaatgccact
    12361 ggcattaggt cattggggga tttgttcggt ctgctcacgc gccgcccgta cagataaaac
    12421 gttatgccca caatgtggat tacccgccac acactcccat cttccctgcg gtcgctgcct
    12481 gcaaaaaccg ccgccctggc aaagactggt cacggttgcc gactatgcgc cgccgttaag
    12541 tctgctcatc caccagctta agttttcccg gcgcagcgaa atcgccagcg ccctgtcacg
    12601 tctgttacta ttggaagtct tacacgctcg tcgcaccacc gggttgcaat tgccggatcg
    12661 tatcatcagc gttccgttat ggcagcggcg tcactggcgt cggggattta atcagagcga
    12721 tttgctgtgt cagccgttat cacgctggtt gcactgccaa tgggatagcg aagccatcac
    12781 acgtacacgg gccactgcga cccagcattt tcttagcgcc cggctacgca ggcgcaacct
    12841 gaaaaatgcc tttcgccttg aattgcctgt gcaaggtcgc catatggtga ttgtggatga
    12901 tgtcgttacc accggaagta ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc
    12961 ggcgactgtc caggtctggt gcctttgtcg aaccttgtag agcctcgatg atgggcgtat
    13021 tataaccaac taaaatagtc aactattagg ccattactat gatccgtatt tccgatgctg
    13081 cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga agggacacaa atccgcgtat
    13141 ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt ttcttattgt ccgccggatg
    13201 ccgtggaagc caccgacacc gccctgaaat ttgacctgct gaccgcgtat gttgatgagt
    13261 taagcgcacc atacctggaa gatgcagaga tcgattttgt taccgaccag ttgggttctc
    13321 agctaacact gaaagcgcct aacgccaaaa tgcgtaaagt ggcagacgat gcaccactga
    13381 tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg
    13441 gccgcgtttc actgatggaa atcaccgaag acggttacgc cattctgcaa tttggcggcg
    13501 gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga agggatcgag aagcagctac
    13561 taaacgaatt cccggagctg aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac
    13621 actcctacta ctaagttatc ccctcttttg tagattgccc gatgcgacgc taaagcgtca
    13681 tttcgggcct acgttgaaca tgtgccagac gttggcgcag cattgctttg gcaaatccct
    13741 cccttcgttt ccccgcgata atatgaccaa cctctcataa tttaaattta ccccgctctg
    13801 gtgattctca aacgccagat gttacccgta tcattcacgt gggtaccaaa catactcctg
    13861 acatctgact acaataatta gttttagtgg gtatcagtcg tggtgccgca atatctctgt
    13921 tcccgattgg gataattaga gcttgtcgtc agaaaattga cgttacccat aacaaatgaa
    13981 aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttattactcc
    14041 tgatgatccg cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgcggttg
    14101 gattaatgca agggatgccg ctggataaag ttattggctc catcaaagcc ggtgtcggcg
    14161 ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc aatgctgggc aaaatgctgg
    14221 cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat tgccaaattt ggtaaaaaac
    14281 acatccagtg ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag
    14341 tgggctttgt gctgatgctg ccgctggtgt ttaccatcgc ggcttctgcg aatattccac
    14401 tgctgtatgt tggtgtacca atggcagctg cactgtctgt gacccacggc ttcctgccac
    14461 cgcatccggg tccgactgcg attgccacca ttttcaatgc cgatatgggt aaaaccctgc
    14521 tgtacggtac tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg
    14581 tgttgaaagg tatcgataag ccaattccgg aaggtctcta cagcgcgaaa accttcagcg
    14641 aagaagagat gccgagcttt ggcgtcagcg tctggacctc tctggtgccg gtagtactga
    14701 tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag
    14761 cggagttcct cggtgacccg gtaatggcta cgctgattgc cgtgctgatt gcgatgttca
    14821 cctttggtct gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca
    14881 tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcaggtgc
    14941 tggtagacag cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc
    15001 cgctgctgat ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg
    15061 ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc
    15121 cggagctgat ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc
    15181 cgggcttctg gctgttcaaa gagtacttta acctgactat cggcgagacc atcaaatcct
    15241 ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata
    15301 tggtgatttg ataacactga ctgccggatg catcgcatcc ggcatcaacc gcactctact
    15361 tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat
    15421 catcggcaaa catcgtctca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt
    15481 aactggtgcc aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc
    15541 ctaacagagc actgttactg tcggcaatgt agcgctgcaa accacgatta agcgtcggcg
    15601 tcatcgacat caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca
    15661 gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca
    15721 cttcatccgg ataccgcccc agggttttgc ccagcgttag atccccgctc tcccagtagc
    15781 cgcgtagcgt aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt
    15841 tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca
    15901 caccgctgct gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa
    15961 tcaccataca gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg
    16021 gatagtgaac atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca
    16081 gcatcgacat cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca
    16141 gctcgataaa cggttcataa gcacgcgcgg tgatgatatg cgggtccatt ggcggcaatc
    16201 cccagttctg ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc
    16261 aatacagttc acggtcgcac caggtttccg ccccaccttc cgctacgcca accgccagat
    16321 cacgatacaa gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact
    16381 ggctgtaagc ctgccactgc aaccagagat aaaaatcaac gtcatcgcga tgttcttcgc
    16441 agaactgacg cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc
    16501 cccagcgcat ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc
    16561 agaacaggct gtcgccttga tctgcaacaa actggcgaaa cgcggccatc tgctcatcat
    16621 cacgttgcgc gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg
    16681 tggagtaatc gacccagtcg gcatcccgcg cctgttgcag cgtctgttgc gtggtcggca
    16741 actgccacca ggcctgagcc tcttcgctaa gatggaaatc ttcgacggcg ttaacgtcga
    16801 tataaatcac attcagccag cggcgagaag acgggctata cgggctggcg ctctccggat
    16861 ttgccggata gagcgcatga atcgggttca gaccaatgaa cgatccgcca cgttttgcca
    16921 catccactag catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc
    16981 gcagcgtata aagctggacg caggcacccc acagcttttg tttattcagc aacgcctgcg
    17041 gttcgtaaca gcgtttcggg gcgacaatca ccctgcaatg cccgcgctgg tcttcctggg
    17101 taagcgtcag cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc
    17161 ccgttatatg gcctttgtac tgcgttcctt cttcggtggt cagcagccag ctatattcgc
    17221 cgctgccctc caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga
    17281 ctggcgttac cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt
    17341 tggtttcggc gctaatcgac tgcggtttac cgtgggcatt gatgtaatga gggctaatcc
    17401 ccgccgccag cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg
    17461 cctgccagat acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc
    17521 gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca
    17581 cctgcttttg tgcctctaca taggctgcga aatccgccat caccagatac ggatcgccgc
    17641 cctgtttgcc gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt
    17701 taccgctttc cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca
    17761 ccgggtcgta gcctttggcc agaattgcct tcacttgttc cacggtatga ccaaaaataa
    17821 agatattttc ttcaccgact ttctcggcga tttcaacgtt cgccccatcc agcgtaccga
    17881 cagtaagcgc accattgagc gccagtttca tattgccggt accggaagct tctttacctg
    17941 cagtcgaaat ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc
    18001 aataatccgg caggaacacc accttcaact tatcgccaac cagcggatcg ttgttgatca
    18061 cgtcagccac tttgttgatc gcaaagataa tatttttcgc caggtagtag cccggtgccg
    18121 ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc ctgtgggttt tcacgaattt
    18181 ctttgtacaa cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac
    18241 gtttgatctg aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca
    18301 caaactccgc cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt
    18361 tcgcatcatc agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt
    18421 ttttcagtga tttatccaac agagccgcca gtgccgggtt gcactgtttg atccagcgac
    18481 gtggggtaat accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg
    18541 ggaacagatc tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac
    18601 cgccaaccac acacaggttc gccatatgca cttgtttgtc gtgcaccacc gccagtttag
    18661 cccacacttt ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa
    18721 tttcgttaat aatctgcatg tggcgcggca gtaagccttt caccagtttc acatcccagc
    18781 gttccagcgc ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatgg
    18841 cccaggcgtc atcccagctc atctggtgct catcgatcag cacgcgcaac agttctggaa
    18901 tcgcgatagt tgggtgggta tcgttcagct gaataacttc gtaatccgcc agttcgtgca
    18961 gtttacgccc cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga
    19021 agtactgctg catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa
    19081 ctttggtcag tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt
    19141 taaatttagt cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg
    19201 ccacgccatt acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact
    19261 ccggctccca gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg
    19321 cttcgttgtg gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct
    19381 gtttgccatc cacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg
    19441 tcgcagactg accgacagtt gccattgaat cgaggaagca cgccgccaga cgtcccagac
    19501 caccgttacc cagcgcaggg tcgatctctt cttccagcag gtcagtcaga ttgatgtcat
    19561 aagccttcaa cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg
    19621 tcagacgacc aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg
    19681 gcttggcgaa tggctgagca cgcagcattt cggccagtgc ttcactcact gccagccacc
    19741 actggcgagg agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa
    19801 gcgcttcctg aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct
    19861 tagattttca acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc
    19921 tgcgggggat taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcgaccac
    19981 ttaaagctag tgcaaaccac aggattagca gcaaatcaat gccatacagc gcagaaaatc
    20041 tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa aagcgaaagg taactctata
    20101 aattaagtaa aggagtgaaa cagtttcaca agtaaaatat ccagtgtgct ccatctcatt
    20161 cttaatagat ttattaagat catcttttta gatagcactt tcatcaggaa tgaagaagaa
    20221 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
    20281 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
    20341 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
    20401 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
    20461 attccgtcaa aattaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
    20521 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacaag tcctgcgggc
    20581 tacggtaaga ccacgctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
    20641 tcgctggacg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccgccgtg
    20701 caacaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
    20761 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca
    20821 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
    20881 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgtaacctt
    20941 ccgcaactgg gcattgccaa tctgcgtgtt cgtgatcaac tgctggaaat tggcagtcag
    21001 caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
    21061 attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
    21121 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
    21181 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
    21241 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
    21301 gcactcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgcctcga agagattgag
    21361 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
    21421 ctgtttggta acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
    21481 atccaccgtg ccgccgcaga aagctggatg gcccagggat tccccagcga agcgattcat
    21541 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
    21601 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
    21661 gacagtttgc tggaaaatcc gcagttggtg ttattgcagg cgtggctgat gcaaagccaa
    21721 catcgctacg gcgaagttaa caccctgctg gcccgtgctg aacatgaaat caaggacatc
    21781 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
    21841 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct accgccgggc
    21901 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
    21961 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
    22021 gtctggcact acgccttatg gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg
    22081 ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat
    22141 ctggaacagt tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
    22201 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttatcgtct
    22261 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
    22321 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
    22381 aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg
    22441 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
    22501 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
    22561 gagtttgaac ctgcggaaat tgtcctcgaa gaactcaatg aaaatgcccg cagtctgcgg
    22621 ttgatgagcg atctcaaccg taacctgttg ctgctaaatc aactgtactg gcaggccgga
    22681 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
    22741 tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
    22801 caactcaata cgctgccgga actggaacag catcgcgcac agcgtattct acgagaaatc
    22861 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgcta
    22921 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
    22981 gtcctggggc tgatttactc cggttacagc aacgaacaaa tagcgggtga actggaagtg
    23041 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
    23101 cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga
    23161 gtttgtatag cgtttttatg tacactcttt atactgtata taaaatcgca ctacaggctc
    23221 atgaatatga aaccggaaag caaagaagcg ccaatcaata tcagagcgaa agcctcacag
    23281 cgtgacctga ttgatatggc agcgaatctg gtcgcaaaat cgcgcactga ttttatgctg
    23341 gatgccgcat gccgtgaagc gcaagacatc ttattagatc aacgactatt catccttgat
    23401 gacgagcaat acgatgcgtt tctcgctgcc ctggacgccc ccatcactgc tgagcgtcag
    23461 gccaaaatta acgcgttgat gaatcgtaaa tcgccatggg aataacggct cctactcccc
    23521 taacgtctga acataatctg gctgactttt gctgttccga tcacgggatg aatgaatggc
    23581 taaaaaagaa agcgttaaaa aatcacagct ccggtctgtc tcgtgtttac gttatctgta
    23641 ttgccaatac ccgccaggta atcggctatt actgcctttc tacaggcagt attcaacgta
    23701 atttggcccc tggagccatg cgacgtaatg ctcccgaatc gttgcctgtt gtcgtattag
    23761 gcagactggc aattgatcaa gcctgggcag gtaaagggtt gggcgtcgca ttgttgaaag
    23821 atgcagtcta tcgcacaatg tccatagctc aacaggtagg ggttcgtgcg ctcattgttc
    23881 atgccttaga cgattcagta cgcaatttct atctgaagta tgcctttgta ccttcaccat
    23941 tccagtcact taccttactg tatcccatta ctctggagta atttacctct accggatagt
    24001 cacactgatg agtctgatcc gggagggatt tcaatcagtg agtttggtta tcatcacccg
    24061 cgttacgcca tctgtttcag ccagagtaaa ccgcaccggc aagaaacgct ccaccacggc
    24121 gatattggtc agcagatggc atgagggatg ggcgaccgta aattgtcccg cgcccgccag
    24181 cgccattggc aacactagct ggtcagcaag atattccccc actgccgccg ggcttgccag
    24241 gtagcgtttc acctctttca ccaactgtgc cgcaaccacc tcggcactga cgcgcttttc
    24301 accgacgaca aaaaagcgtt cggtgatatt ttcactttcg acttcaagcg agacggtatt
    24361 acccggcccc tggtcgcgcg gcaggttatg aatattctgc tcatgcaggg aaaaacttgc
    24421 cgccagcgta gcgatttcac gctcagcgac atgccgcggc acgccagcta ataacacctc
    24481 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
    24541 cggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaacag
    24601 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccaacaccc ggcggataaa
    24661 atccgcaggc ggggccgacg ggttatcggt accgccgctc acttcaacac gcgaaggtcc
    24721 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacaac ttcctgcgct
    24781 accgatagca aagcgataat cgccgccacg cacggtgccg ggccggaaga ccagacgctg
    24841 cgaccccagc tccgcgcctt ccaccgtcgc cccgcaaatt tccgtcgccg cctttaccgc
    24901 cgtcagatgc tggcgcaaca gtcccggttt tgcccgcccg gcacgaatgc cggtgatggt
    24961 aaatggctgg ccggttatca tcgacagact cagcgccgag cgcatgatct gcccgccgcc
    25021 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
    25081 cacctgacgt agggtgtaga taacttccac cagatcgctt tgtgccgcca tcaccgcatc
    25141 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
    25201 atgcacggtg gcacgaattt gatcttccac gctgaacatt tttttcgctt tagtacggct
    25261 cataacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
    25321 gacgataaag cttttcgctc ccatcgaccc tggaataatt ccatattgac cagcacgcgc
    25381 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
    25441 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
    25501 ctgcgtaatg ctctgcaacg ccgttactac gttttccatc atcacatcgc ggttaaggct
    25561 ggcaaaaagc tgcgcccagg ccacggcttt caggtaatca tcaaagtatt ccgtaccttc
    25621 cataaagtac gccagatcac gcgacggcaa tgtttcaagc gtttcctgca tctctttttg
    25681 cgccagatcg ataaagtaag tcccgatggc attaccaatt ccgcgtgaac cggagtgcag
    25741 cataatccac acctggtccg actcatcaag gcagatttca ataaagtggt tacccgttcc
    25801 cagcgttccc aggtgtttat agttgttagt gttaaggaaa cgcggatatt tttgcgttaa
    25861 ccactgataa ccggcctcca gctcagccca tttcgcatcg acgttaacag gcgggttttc
    25921 ccatgcgcct ttatcacgtt tacaacggcc agtggtacgc ccgtgcggca ccgcagtttc
    25981 aatcgcctga cgcagctccg ccaggttttc cggcaggtca gccgccgtta aagcggtacg
    26041 tagtgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
    26101 ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag gcattaccgc
    26161 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
    26221 ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc
    26281 gtaattcatt ttgtttttct cttttcgtt
//
LOCUS       DAZFKY010000053        26212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFKY010000053 DAZFKY010000000
VERSION     DAZFKY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..159
                     /gene="tuf"
                     /locus_tag="ACHKCL_001925"
     CDS             <1..159
                     /gene="tuf"
                     /locus_tag="ACHKCL_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5762896.1"
                     /translation="ELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGA
                     GVVAKVLG"
     gene            342..536
                     /gene="bfd"
                     /locus_tag="ACHKCL_001926"
     CDS             342..536
                     /gene="bfd"
                     /locus_tag="ACHKCL_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5762897.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            609..1085
                     /gene="bfr"
                     /locus_tag="ACHKCL_001927"
     CDS             609..1085
                     /gene="bfr"
                     /locus_tag="ACHKCL_001927"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5762898.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(1082..1549)
                     /locus_tag="ACHKCL_001928"
     CDS             complement(1082..1549)
                     /locus_tag="ACHKCL_001928"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5762899.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1928..2239
                     /gene="rpsJ"
                     /locus_tag="ACHKCL_001929"
     CDS             1928..2239
                     /gene="rpsJ"
                     /locus_tag="ACHKCL_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5762900.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2272..2901
                     /gene="rplC"
                     /locus_tag="ACHKCL_001930"
     CDS             2272..2901
                     /gene="rplC"
                     /locus_tag="ACHKCL_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5762901.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2912..3517
                     /gene="rplD"
                     /locus_tag="ACHKCL_001931"
     CDS             2912..3517
                     /gene="rplD"
                     /locus_tag="ACHKCL_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5762902.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3514..3816
                     /gene="rplW"
                     /locus_tag="ACHKCL_001932"
     CDS             3514..3816
                     /gene="rplW"
                     /locus_tag="ACHKCL_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5762903.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3834..4655
                     /gene="rplB"
                     /locus_tag="ACHKCL_001933"
     CDS             3834..4655
                     /gene="rplB"
                     /locus_tag="ACHKCL_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5762904.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4672..4950
                     /gene="rpsS"
                     /locus_tag="ACHKCL_001934"
     CDS             4672..4950
                     /gene="rpsS"
                     /locus_tag="ACHKCL_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5762905.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4965..5297
                     /gene="rplV"
                     /locus_tag="ACHKCL_001935"
     CDS             4965..5297
                     /gene="rplV"
                     /locus_tag="ACHKCL_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5762906.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5315..6016
                     /gene="rpsC"
                     /locus_tag="ACHKCL_001936"
     CDS             5315..6016
                     /gene="rpsC"
                     /locus_tag="ACHKCL_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5762907.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6029..6439
                     /gene="rplP"
                     /locus_tag="ACHKCL_001937"
     CDS             6029..6439
                     /gene="rplP"
                     /locus_tag="ACHKCL_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5762908.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6439..6630
                     /gene="rpmC"
                     /locus_tag="ACHKCL_001938"
     CDS             6439..6630
                     /gene="rpmC"
                     /locus_tag="ACHKCL_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5762909.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6630..6884
                     /gene="rpsQ"
                     /locus_tag="ACHKCL_001939"
     CDS             6630..6884
                     /gene="rpsQ"
                     /locus_tag="ACHKCL_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5762910.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7049..7420
                     /gene="rplN"
                     /locus_tag="ACHKCL_001940"
     CDS             7049..7420
                     /gene="rplN"
                     /locus_tag="ACHKCL_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5762911.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7431..7745
                     /gene="rplX"
                     /locus_tag="ACHKCL_001941"
     CDS             7431..7745
                     /gene="rplX"
                     /locus_tag="ACHKCL_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5762912.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7760..8299
                     /gene="rplE"
                     /locus_tag="ACHKCL_001942"
     CDS             7760..8299
                     /gene="rplE"
                     /locus_tag="ACHKCL_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5762913.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8314..8619
                     /gene="rpsN"
                     /locus_tag="ACHKCL_001943"
     CDS             8314..8619
                     /gene="rpsN"
                     /locus_tag="ACHKCL_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5762914.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8653..9045
                     /gene="rpsH"
                     /locus_tag="ACHKCL_001944"
     CDS             8653..9045
                     /gene="rpsH"
                     /locus_tag="ACHKCL_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5762915.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9058..9591
                     /gene="rplF"
                     /locus_tag="ACHKCL_001945"
     CDS             9058..9591
                     /gene="rplF"
                     /locus_tag="ACHKCL_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5762916.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9601..9954
                     /gene="rplR"
                     /locus_tag="ACHKCL_001946"
     CDS             9601..9954
                     /gene="rplR"
                     /locus_tag="ACHKCL_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5762917.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9969..10472
                     /gene="rpsE"
                     /locus_tag="ACHKCL_001947"
     CDS             9969..10472
                     /gene="rpsE"
                     /locus_tag="ACHKCL_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5762918.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10476..10655
                     /gene="rpmD"
                     /locus_tag="ACHKCL_001948"
     CDS             10476..10655
                     /gene="rpmD"
                     /locus_tag="ACHKCL_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5762919.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10659..11093
                     /gene="rplO"
                     /locus_tag="ACHKCL_001949"
     CDS             10659..11093
                     /gene="rplO"
                     /locus_tag="ACHKCL_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5762920.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11101..12432
                     /gene="secY"
                     /locus_tag="ACHKCL_001950"
     CDS             11101..12432
                     /gene="secY"
                     /locus_tag="ACHKCL_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5762921.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12464..12580
                     /gene="rpmJ"
                     /locus_tag="ACHKCL_001951"
     CDS             12464..12580
                     /gene="rpmJ"
                     /locus_tag="ACHKCL_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5762922.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12727..13083
                     /gene="rpsM"
                     /locus_tag="ACHKCL_001952"
     CDS             12727..13083
                     /gene="rpsM"
                     /locus_tag="ACHKCL_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5762923.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13100..13489
                     /gene="rpsK"
                     /locus_tag="ACHKCL_001953"
     CDS             13100..13489
                     /gene="rpsK"
                     /locus_tag="ACHKCL_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5762924.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13523..14143
                     /gene="rpsD"
                     /locus_tag="ACHKCL_001954"
     CDS             13523..14143
                     /gene="rpsD"
                     /locus_tag="ACHKCL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5762925.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14169..15158
                     /gene="rpoA"
                     /locus_tag="ACHKCL_001955"
     CDS             14169..15158
                     /gene="rpoA"
                     /locus_tag="ACHKCL_001955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5762926.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15199..15582
                     /gene="rplQ"
                     /locus_tag="ACHKCL_001956"
     CDS             15199..15582
                     /gene="rplQ"
                     /locus_tag="ACHKCL_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5762927.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15689..16057
                     /gene="yhdN"
                     /locus_tag="ACHKCL_001957"
     CDS             15689..16057
                     /gene="yhdN"
                     /locus_tag="ACHKCL_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5762928.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16068..16493
                     /gene="zntR"
                     /locus_tag="ACHKCL_001958"
     CDS             16068..16493
                     /gene="zntR"
                     /locus_tag="ACHKCL_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5762929.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16549..16767
                     /gene="arfA"
                     /locus_tag="ACHKCL_001959"
     CDS             16549..16767
                     /gene="arfA"
                     /locus_tag="ACHKCL_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5762930.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16764..17174)
                     /gene="mscL"
                     /locus_tag="ACHKCL_001960"
     CDS             complement(16764..17174)
                     /gene="mscL"
                     /locus_tag="ACHKCL_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5762931.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17304..18680)
                     /gene="trkA"
                     /locus_tag="ACHKCL_001961"
     CDS             complement(17304..18680)
                     /gene="trkA"
                     /locus_tag="ACHKCL_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5762932.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18702..19991)
                     /gene="rsmB"
                     /locus_tag="ACHKCL_001962"
     CDS             complement(18702..19991)
                     /gene="rsmB"
                     /locus_tag="ACHKCL_001962"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5762933.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20037..20984)
                     /gene="fmt"
                     /locus_tag="ACHKCL_001963"
     CDS             complement(20037..20984)
                     /gene="fmt"
                     /locus_tag="ACHKCL_001963"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5762934.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20999..21508)
                     /gene="def"
                     /locus_tag="ACHKCL_001964"
     CDS             complement(20999..21508)
                     /gene="def"
                     /locus_tag="ACHKCL_001964"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5762935.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21638..22762
                     /gene="dprA"
                     /locus_tag="ACHKCL_001965"
     CDS             21638..22762
                     /gene="dprA"
                     /locus_tag="ACHKCL_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5762936.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22734..23207
                     /gene="smg"
                     /locus_tag="ACHKCL_001966"
     CDS             22734..23207
                     /gene="smg"
                     /locus_tag="ACHKCL_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5762937.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23236..23778
                     /gene="yrdD"
                     /locus_tag="ACHKCL_001967"
     CDS             23236..23778
                     /gene="yrdD"
                     /locus_tag="ACHKCL_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5762938.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23783..24355
                     /gene="tsaC"
                     /locus_tag="ACHKCL_001968"
     CDS             23783..24355
                     /gene="tsaC"
                     /locus_tag="ACHKCL_001968"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5762939.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24360..25178
                     /gene="aroE"
                     /locus_tag="ACHKCL_001969"
     CDS             24360..25178
                     /gene="aroE"
                     /locus_tag="ACHKCL_001969"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5762940.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25175..25432
                     /gene="yrdB"
                     /locus_tag="ACHKCL_001970"
     CDS             25175..25432
                     /gene="yrdB"
                     /locus_tag="ACHKCL_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5762941.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25408..25962)
                     /gene="yrdA"
                     /locus_tag="ACHKCL_001971"
     CDS             complement(25408..25962)
                     /gene="yrdA"
                     /locus_tag="ACHKCL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5762942.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6719 a         6215 c         6810 g         6468 t
ORIGIN      
        1 gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca acatcaaaat ggttgttacc
       61 ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg caatccgtga aggcggccgt
      121 accgttggcg cgggcgttgt agctaaagtt ctgggctaat tacacgttaa ttagttttga
      181 attgaaaagg gcgcttcggc gccctttttg ctgcccgtaa tttgagctcg aaatatttag
      241 tcgtaatttt gctcgctgaa ctatggcgca aataaaactg tgctattgta atcataacta
      301 ttctcattta cactttgcgc agaatttgaa cgggagcaat catgtacgtt tgtttgtgta
      361 atggtgtaag tgacaaaaaa attcgccagg cagtccgtca gtttcatccg caatctttcc
      421 aacaattgcg taagtttatt cctgtgggaa atcaatgtgg taagtgcatt cgcgccgcgc
      481 gcgaagtgat gcaggacgag ttgatgcaga tgccagaatt taaagagatt gcctgaagcc
      541 cacgcttatt ttttgacatc ccgctagccc catctacgct ctaatagtgg aagcggaggg
      601 actataaaat gaaaggtgat gttaaaatca taaattatct caataaacta ttgggaaatg
      661 agcttgtcgc aatcaatcag tattttctcc atgcccggat gtttaaaaac tggggactga
      721 tgcgtcttaa tgacgtcgaa taccatgaat ccatcgatga gatgaaacac gctgataaat
      781 atatcgagcg tattttgttt ttagaaggaa tccccaactt acaggattta ggtaagctag
      841 ggatcggtga agatgtcgaa gagatgctgc aatccgacct gcgattagag ctggaaggtg
      901 cgaaggatct gcgtgaggct atcgcgtatg ccgacagcgt tcatgactat gttagccgcg
      961 atatgatgat cgagatcctt gccgatgaag aaggtcatat cgactggctc gaaactgaac
     1021 tggatctcat cggtaaaatc ggcctacaaa attacctgca atcacaaatt aaagttaatg
     1081 attaacggcc tatcagcaaa ctatcccagc ctataacgaa acccgcagcc gccagaaatg
     1141 gcccaaaagg cagcgggttt tttaatgatt cttttcctct catgagcaga ccaataacta
     1201 cggctccgca agcgaatgag gcagcaagga aaaccagtcg tggtaagaag gcccatgaat
     1261 gccaggcgcc aagggcggcg agaaacttca catcaccata gcctaatcct tctttatggc
     1321 gcagtatgcg atacccccag taaatgacag cgaatgtgcc gtaaccaata attgcccccc
     1381 ataacgcatc ggctaaacca tcaggatgac aaacttgata gaacaaaaga cctgaccaga
     1441 gtaacgggca cgtaaatctg tcaggtaaca agccatgctt tgcatcccag aaaaaaagta
     1501 gggctgataa acaagcgtac aaaattagaa agggaagggt agtgtccata aaacatcctc
     1561 tgtaattgga gtatcaccat cgaataactc cggatgctca gcaacagaga gagcgctgat
     1621 ttgttaacct gattccggtg aaaaacgcta tcttcacaac cttacaaatt tcgtggaatg
     1681 cagctcgcaa tctgcgcgtt gcgattaatt tctgcccgat gcttgcgtca ggctcagatt
     1741 tgcgtataat gcgcgggctt gtcgtagttg acagcaggtt caatctgaac ctcagttagc
     1801 cgaatttggc tacctaacaa tgctcccaat cggggagcta cgtaagaacg gttacactct
     1861 cccatcaatc gtaatgggtc tgaggagtaa tcattttcgt ttataaaata attggagctc
     1921 tggtctcatg cagaaccaaa gaatccgtat ccgcctgaaa gcgtttgatc atcgtctgat
     1981 cgatcaagca accgcggaaa tcgtcgagac tgccaagcgc actggtgcgc aggtccgtgg
     2041 tccgatcccg ctgccgacac gcaaagagcg cttcactgtt ctgatctccc cgcacgtcaa
     2101 caaagacgcg cgcgatcagt acgaaatccg tactcacttg cgtctggttg acatcgttga
     2161 gccaaccgag aaaaccgttg atgctctgat gcgtctggat ctggctgccg gtgtagacgt
     2221 gcagatcagc ctgggttaat caggtcattg agcgattgag aggttgaaac aatgattggt
     2281 ttagtcggta aaaaagtggg tatgacccgt atcttcacag aagacggcgt ttctatccca
     2341 gtaaccgtaa tcgaagttga agcaaaccgc gttactcagg ttaaagacct ggctaacgat
     2401 ggctaccgtg ctattcaggt gaccaccggt gctaaaaaag ctaaccgtgt gaccaagcct
     2461 gaagctggcc acttcgctaa agctggcgta gaagctggcc gtggtctgtg ggaattccgc
     2521 ctggctgaag gcgaagagtt cactgtaggt cagagcatta gcgttgaact gtttgctgac
     2581 gttaaaaaag ttgacgtaac tggcacctct aaaggtaaag gtttcgcagg taccgttaag
     2641 cgctggaact tccgtaccca agacgctact cacggtaact ccttgtctca ccgcgttccg
     2701 ggttctatcg gtcagaacca gactccgggc aaagtgttca aaggcaagaa aatggcaggt
     2761 cagatgggta acgaacgtgt aaccgttcag agccttgacg tagtacgcgt tgacgctgag
     2821 cgcaacctgc tgctggttaa aggtgctgtc ccgggtgcaa ccggtagcga cctgatcgtt
     2881 aaaccagctg tgaaggcgta aggagatagc aatggaatta gtattgaaag acgcgcagag
     2941 cgcgctgact gtttccgaaa ctaccttcgg tcgtgatttc aacgaagcgc tggttcacca
     3001 ggttgttgtt gcttatgcag ctggtgctcg tcagggtact cgtgctcaga agactcgtgc
     3061 tgaagtaact ggttccggta aaaaaccgtg gcgccagaaa ggcaccggcc gtgcgcgttc
     3121 tggttctatc aagagcccga tctggcgttc tggtggcgtg acctttgctg ctcgtccgca
     3181 ggaccacagt caaaaagtta acaagaagat gtaccgcggc gcgctgaaaa gcatcctgtc
     3241 cgaactggta cgtcaggatc gtctgatcgt tgtcgagaag ttctctgtag aagcgccgaa
     3301 aactaagctg ctggcacaga aactgaaaga catggctctg gaagatgtgc tgatcatcac
     3361 cggtgagctg gacgaaaacc tgttcctggc tgcgcgcaac ctgcacaagg ttgacgtacg
     3421 cgatgcaact ggtatcgacc cggttagcct gatcgccttc gacaaagtcg taatgactgc
     3481 tgatgctgtt aagcaagttg aggagatgct ggcatgattc gtgaagaacg tctgctgaag
     3541 gtgctgcgtg caccgcacgt ttctgaaaaa gcgtctactg cgatggaaaa atccaacacc
     3601 atcgtactca aagttgctaa agacgcgacc aaagcagaaa tcaaagctgc tgtgcagaaa
     3661 ctgtttgaag tcgaagtcga agtcgttaac accctggtag ttaaagggaa agttaaacgt
     3721 cacggacagc gtatcggtcg tcgtagcgac tggaaaaaag cttacgtcac cctgaaagaa
     3781 ggccagaatc tggacttcgt tggcggcgct gagtaagtcg gaggagtaat acaatggcag
     3841 ttgttaaatg taaaccgaca tctccgggtc gtcgccacgt agttaaagtg gttaaccctg
     3901 agctgcacaa gggcaaacct tttgctccgt tgctggaaaa aaacagcaaa tccggtggtc
     3961 gtaacaacaa tggccgtatc accactcgtc atatcggtgg tggccacaag caggcttacc
     4021 gtattgttga cttcaaacgc aacaaagacg gtatcccggc agttgttgaa cgtcttgagt
     4081 acgatccgaa ccgttccgcg aacatcgcgc tggttctgta caaagacggt gaacgccgtt
     4141 acatcctggc ccctaaaggc ctgaaagctg gcgaccagat tcagtctggc gttgatgctg
     4201 caatcaaacc aggcaacacc ctgccgatgc gcaacatccc ggttggttct actgttcata
     4261 acgtagaaat gaaaccaggt aaaggcggtc agctggcacg ttccgctggt acttacgttc
     4321 agatcgttgc tcgcgacggt gcttatgtca ccctgcgtct gcgttctggt gaaatgcgta
     4381 aagtcgaagc agactgccgt gcaactctgg gcgaagttgg caatgctgag catatgctgc
     4441 gcgttctggg taaagcaggt gctgcacgct ggcgtggtgt tcgtccgacc gttcgcggta
     4501 ccgcgatgaa cccggtagac cacccacatg gtggtggtga aggtcgtaac tttggtaagc
     4561 acccggtaac tccgtggggc gttcagacca aaggtaagaa gacccgcagc aacaagcgta
     4621 ctgataaatt catcgtacgt cgccgtagca aataatttta gaggataagc catgccacgt
     4681 tctctcaaga aaggtccttt tattgacctg cacttgctga agaaggtaga gaaagcggtg
     4741 gaaagcggag acaagaagcc cctgcgcact tggtcccgtc gttcaacgat ctttcctaac
     4801 atgatcggtt tgaccatcgc tgtccataat ggtcgtcagc acgttccggt atttgtaacc
     4861 gacgaaatgg tcggtcacaa actgggtgaa ttcgcaccga ctcgtactta tcgcggccac
     4921 gctgctgata aaaaagcgaa gaagaaataa ggtaggagga agagatggaa actatcgcta
     4981 aacatcgcca tgctcgttct tctgctcaga aggttcgcct tgttgctgac ctgattcgcg
     5041 gtaagaaagt gtcgcaggct ctggatattt tgacctacac caacaagaaa gcggctgtac
     5101 tggtcaagaa agttctggaa tctgccattg ctaacgctga acacaacgat ggcgctgaca
     5161 ttgacgatct gaaagttacg aaaattttcg tagacgaagg cccgagcatg aagcgcatta
     5221 tgccgcgtgc aaaaggtcgt gcagatcgca tcctgaagcg caccagccac atcactgtgg
     5281 ttgtgtccga tcgctgagac tctggagact agcaatgggt cagaaagtac atcctaatgg
     5341 tattcgcctg ggtattgtaa aaccatggaa ctctacctgg tttgcgaaca ccaaagaatt
     5401 cgctgacaac ctggacagcg attttaaagt acgtcagtac ctgactaagg aactggctaa
     5461 agcgtccgta tctcgtatcg ttatcgagcg tccggctaag agcatccgtg taaccattca
     5521 cactgctcgc ccgggtatcg ttatcggtaa aaaaggtgaa gacgtagaaa aactgcgtaa
     5581 ggtcgtagcg gacatcgctg gcgttcctgc acagatcaac atcgccgaag ttcgtaagcc
     5641 tgaactggac gcaaaactgg ttgctgacag catcacttct cagctggaac gtcgcgttat
     5701 gttccgtcgt gctatgaagc gtgctgtaca gaacgcaatg cgtctgggcg ctaaaggtat
     5761 taaagttgaa gttagcggcc gtctgggcgg cgcggaaatc gcacgtaccg aatggtaccg
     5821 cgaaggtcgc gtaccgctgc acactctgcg tgctgacatc gactacaaca cctctgaagc
     5881 gcacaccact tacggtgtaa tcggcgttaa agtgtggatc ttcaaaggcg agatcctggg
     5941 tggtatggct gctgttgaac aaccggaaaa accggctgct cagcctaaaa agcagcagcg
     6001 taaaggccgt aaataaggag cgtcgctgat gttacaacca aagcgtacaa aattccgtaa
     6061 aatgcataaa ggccgtaacc gcggtctggc gcagggtacg gatgttagct tcggcagctt
     6121 cggtctgaaa gctgttggcc gtggtcgtct gactgcccgt cagatcgaag cagcacgtcg
     6181 tgctatgacc cgtgcagtta agcgtcaagg taagatctgg atccgtgtgt tcccggacaa
     6241 accgatcact gaaaagccgc tggcagtgcg tatgggtaaa ggtaaaggta acgtggagta
     6301 ttgggttgcc ttgattcagc cgggtaaagt cctgtatgaa atggacggtg ttccggaaga
     6361 gctggcccgt gaagcattca agctggcagc agcgaaactg ccgattaaaa ccacctttgt
     6421 aactaagacg gtgatgtaat gaaagcaaaa gagctgcgtg agaagagcgt tgaagagctg
     6481 aacaccgagc tgctgaacct gctgcgtgag cagttcaacc tgcgtatgca ggctgcaagt
     6541 ggccagctgc aacagtctca cctgttgaag caagtgcgtc gcgatgtcgc acgcgttaag
     6601 actttactga acgagaaggc gggtgcgtaa tgaccgataa aatccgtact ctgcaaggtc
     6661 gcgttgttag cgacaaaatg gagaaatcca ttgttgttgc tatcgaacgt tttgtgaaac
     6721 acccgatcta cggtaaattc atcaagcgta cgaccaaact gcacgtacat gacgagaaca
     6781 acgaatgcgg tatcggtgac gtggttgaaa tccgcgaatg ccgtccgctg tccaagacta
     6841 aatcctggac gctggttcgc gttgtagaga aagcggttct gtaatacagt acactctctc
     6901 gatacgaata aacggctcag aaatgagccg tttatttttt ctacccatat ccttgaagcg
     6961 gtgttataat gccgcgccct cgatatgggg atttttaacg acctgatttt cgggtctcag
     7021 tagtagttga cattagcgga gcactaaaat gatccaagaa cagactatgc tgaacgtcgc
     7081 cgacaactcc ggtgcacgtc gcgtaatgtg tatcaaggtt ctgggtggct cgcaccgtcg
     7141 ctacgcaggc gtaggcgaca tcatcaagat caccatcaaa gaagcaattc cgcgtggtaa
     7201 ggtcaaaaaa ggtgatgtgc tgaaggcggt agtggtgcgc accaagaagg gtgttcgtcg
     7261 cccggacggt tctgtcattc gcttcgatgg taatgcttgt gttcttctga acaacaacag
     7321 cgagcagcct atcggtacgc gtatttttgg gccggtaact cgtgagcttc gtagtgagaa
     7381 gttcatgaaa attatctctc tggcaccaga agtactctaa ggagcgaatc atggcagcga
     7441 aaatccgtcg tgatgacgaa gttatcgtgt taaccggtaa agataaaggt aaacgcggta
     7501 aagttaagaa tgtcctgtct tccggcaagg tcattgttga aggtatcaac ctggttaaga
     7561 aacatcagaa gccggttccg gccctgaacc aaccgggtgg catcgttgaa aaagaagccg
     7621 ctattcaggt ttccaacgta gcaatcttca atgcggcaac cggcaaggct gaccgtgtag
     7681 gctttagatt cgaagacggc aaaaaagtcc gtttcttcaa gtctaacagc gaaactatca
     7741 agtaatttgg agtagtacga tggcgaaact gcatgattac tacaaagacg aagtagttaa
     7801 aaaactcatg actgagttta actacaattc tgtcatgcaa gtccctcggg tcgagaagat
     7861 caccctgaac atgggtgttg gtgaagcgat cgctgacaaa aaactgctgg ataacgcagc
     7921 agcagacctg gcagcaatct ccggtcaaaa accgctgatc accaaagcac gcaaatctgt
     7981 tgcaggcttc aaaatccgtc agggctatcc gatcggctgt aaagtaactc tgcgtggcga
     8041 acgcatgtgg gagttctttg agcgcctgat cactattgct gtacctcgta tccgtgactt
     8101 ccgtggcctg tccgctaagt ctttcgacgg tcgtggtaac tacagcatgg gtgtccgtga
     8161 gcagatcatc ttcccagaaa tcgactacga taaagtcgac cgcgttcgtg gtttggatat
     8221 taccattacc actactgcga aatctgacga agaaggccgc gctctgctgg ctgcctttga
     8281 cttcccgttc cgcaagtaag gtagggttac taaatggcta agcaatcaat gaaagcacgc
     8341 gaagtaaaac gcgtagcttt agctgataaa tacttcgcga aacgcgctga actgaaagcg
     8401 atcatctctg atgtgaacgc ttccgacgaa gatcgttgga acgctgttct caagctgcag
     8461 actctgccgc gtgattccag cccgtctcgt cagcgtaacc gctgccgtca aacaggtcgt
     8521 ccgcatggtt tcctgcggaa gttcgggttg agccgtatta aggtccgtga agccgctatg
     8581 cgcggtgaaa tcccgggtct gaaaaaggct agctggtaat tgtcaccaat tgaatcacgg
     8641 gagtaaagac agatgagcat gcaagatccg atcgcggata tgctgacccg tatccgtaac
     8701 ggtcaggccg cgaacaaagc tgcggtcacc atgccttcct ccaagctgaa agtggcaatc
     8761 gccaacgtgc tgaaggaaga aggttttatt gaagatttta aagttgaagg cgacaccaag
     8821 cctgaactgg aacttactct gaagtatttc cagggcaaag ctgttgtaga aagcattcag
     8881 cgtgtcagcc gcccaggtct gcgcatctat aaacgtaaag atgagctgcc gaaagttatg
     8941 gcgggtctgg gtatcgcagt tgtttctacc tctaaaggtg ttatgactga tcgtgcagcg
     9001 cgccaggctg gtcttggtgg cgaaattatc tgctacgtag cctaatcgga ggaaaaaatg
     9061 tctcgtgttg ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa aatcaacggt
     9121 caggttatta cgatcaaagg taaaaacggc gagctgactc gtactctcaa cgatgctgtt
     9181 gaagttaaac atgcagataa taccctgacc ttcggtccgc gtgatggtta cgcagacggt
     9241 tgggcacagg ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg tgttaccgaa
     9301 ggcttcacta agaagctgca gctggttggt gtaggttacc gtgcagcggt taaaggcaat
     9361 gtgattaacc tgtctctggg tttctctcat cctgttgacc atcagctgcc tgcgggtatc
     9421 actgctgaat gtccgactca gactgaaatc gtgctgaaag gcgctgataa gcaggtgatc
     9481 ggccaggttg cagcggatct gcgcgcctac cgtcgtcctg agccttataa aggcaagggt
     9541 gttcgttacg ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta aggtaacact
     9601 atggataaga aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa gctccaggag
     9661 ctgggcgcaa ctcgcctggt ggtacatcgt accccgcgtc acatttacgc acaggtaatt
     9721 gcaccgaacg gttctgaagt tctggtagct gcttctactg tagaaaaagc tatcgctgaa
     9781 caactgaagt acaccggtaa caaagacgcg gctgcagctg tgggtaaagc tgtcgctgaa
     9841 cgcgctctgg aaaaaggcat caaagatgta tcctttgacc gttccgggtt ccaatatcat
     9901 ggtcgtgtcc aggcactggc agatgctgcc cgtgaagctg gccttcagtt ctaaggtaga
     9961 ggtgtaagat ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag ctgatcgcgg
    10021 taaaccgcgt atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca gctctgactg
    10081 tagttggcga tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt gaagttccag
    10141 cagcgatcca gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc gcgctgaata
    10201 acggcactct gcaacaccct gttaaaggtg ttcacacggg ttctcgcgta ttcatgcagc
    10261 cggcttctga aggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt ctggaagtcg
    10321 ctggggttca taacgttctg gctaaagcat atggttccac caacccgatc aacgtggttc
    10381 gtgcaactat tgatggcctg gaaaatatga attctccaga aatggtcgct gccaagcgtg
    10441 gtaaatccgt tgaagaaatt ctggggaaat aaaccatggc aaagactatt aaaattactc
    10501 aaacccgcag tgcaatcggt cgtctgccga aacacaaggc aacgctgctt ggcctgggtc
    10561 tgcgtcgtat tggtcacacc gtagagcgcg aggatactcc tgctattcgc ggtatgatca
    10621 acgcggtttc cttcatggtt aaagttgagg agtaagagat gcgtttaaat actctgtctc
    10681 cggccgaagg ctccaaaaag gcgggtaaac gcctgggtcg tggtatcggt tctggcctcg
    10741 gtaaaaccgg tggtcgtggt cacaaaggtc agaagtctcg ttctggcggt ggcgtacgtc
    10801 gcggtttcga gggtggtcag atgcctctgt accgtcgtct gccgaaattc ggcttcactt
    10861 ctcgtaaagc agcgattaca gccgaagttc gtctgtctga cctggctaaa gtagaaggcg
    10921 gtgtagtaga cctgaacacg ctgaaagcgg ctaacattat cggtatccag atcgagttcg
    10981 cgaaagtgat cctggctggc gaagtaacga ctccggtaac tgttcgtggc ctgcgtgtta
    11041 ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa taagtagcag
    11101 atggctaaac aaccgggatt agattttcaa agtgccaaag gtggcttagg cgagctgaaa
    11161 cgcagactgc tgtttgttat cggtgcgctg attgtgttcc gtattggctc ttttattccg
    11221 atccctggta ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg aggcaccatc
    11281 attgagatgt ttaacatgtt ctctggtggt gctctcagcc gtgcttctat ctttgctctg
    11341 gggatcatgc cgtatatttc ggcgtcgatc attatccagc tgctgacggt ggttcaccca
    11401 acgttggcag aaattaagaa agaaggggag tctggtcgtc gtaagatcag ccagtacacc
    11461 cgttacggta ctctggtgct ggcaatattc cagtcgatcg gtattgctac cggtctgccg
    11521 aatatgcctg gtatgcaagg cctggtgatt aacccgggct ttgcattcta cttcaccgct
    11581 gttgtaagtc tggtcacagg aacgatgttc ctgatgtggt tgggcgaaca gatcactgaa
    11641 cgaggtatcg gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc gggactcccg
    11701 ccagccattg cccatactat cgagcaagcg cgtcaaggcg acctgcactt cctcgtgttg
    11761 ctgttggttg cagtattagt atttgcagtg acgttctttg ttgtatttgt tgagcgtggt
    11821 caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt ctatgctgca
    11881 cagagcacac atttaccgct gaaagtgaat atggcggggg taatcccggc aatcttcgct
    11941 tccagtatta ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg tactggttgg
    12001 aactggctga caacaatttc gctgtatttg cagcctgggc aaccgcttta tgtgttactc
    12061 tatgcgtctg caatcatctt cttctgtttc ttctacacgg cgttggtttt caacccgcgt
    12121 gaaacagcag ataacctgaa gaagtccggt gcatttgtac caggaattcg tccgggagag
    12181 caaacggcga agtatatcga taaagtaatg acccgcctga ccctggttgg tgcgctgtat
    12241 attaccttta tctgcctgat cccggagttc atgcgtgatg caatgaaagt accgttctac
    12301 ttcggtggga cctcactgct tatcgttgtt gtcgtgatta tggactttat ggctcaagtg
    12361 caaactctga tgatgtccag tcagtatgag tctgcattga agaaggcgaa cctgaaaggc
    12421 tacggccgat aattggtcgc ccgagaagtt acggagagta aaaatgaaag ttcgtgcttc
    12481 cgtcaagaaa ttatgccgta actgcaaaat cgttaagcgt gatggtgtca tccgtgtgat
    12541 ttgcagtgcc gagccgaagc ataaacagcg ccaaggctga ttttttcgca tatttttctt
    12601 gcaaagttgg gttgagctgg ctagattagc cagccaatct tttgtatgtc tgtgcgtttc
    12661 catttgagta tcctgaaaac gggcttttca gcatggaacg tacatattaa atagtaggag
    12721 tgcatagtgg cccgtatagc aggcattaac attcctgatc ataagcatgc cgtaatcgca
    12781 ttaacttcga tttatggcgt cggcaagacc cgttctaaag ccatcctggc tgcagcgggt
    12841 atcgctgaag atgttaagat cagtgagctg tctgaaggac aaatcgacac gctgcgtgac
    12901 gaagttgcca aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat gagcatcaag
    12961 cgcctgatgg atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct cccggttcgc
    13021 ggtcagcgta ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc gatcaagaaa
    13081 taatcggggt gattgaataa tggcaaaggc accaattcgt gcacgtaaac gtgtaagaaa
    13141 acaagtctct gacggcgtgg ctcatatcca tgcttctttc aacaacacca tcgtgactat
    13201 cactgatcgt cagggtaacg cgttgggttg ggcaacagcc ggtggttccg gtttccgtgg
    13261 ttctcgcaaa tccactccgt ttgcagctca ggttgcagca gagcgttgcg ctgacgccgt
    13321 gaaagaatac ggcatcaaga atctggaagt tatggttaaa ggtccgggtc caggccgcga
    13381 atctactatt cgtgctctga acgccgcagg tttccgcatc actaacatta ctgatgtgac
    13441 tccgatccct cataacggtt gtcgtccgcc gaaaaaacgc cgcgtataac gcctcgtttt
    13501 ccaggtttgt tggagaaaga aaatggcaag atatttgggt cctaagctca agctgagccg
    13561 tcgtgagggc accgacttat tccttaagtc tggcgttcgc gcgatcgata ccaagtgtaa
    13621 aattgaacaa gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg actatggtgt
    13681 gcagttgcgt gaaaagcaaa aagttcgccg tatctatggt gtgctggagc gtcagttccg
    13741 taactactac aaagaagcag cacgtctgaa aggcaacacc ggtgaaaacc tgttggctct
    13801 gctggaaggt cgtctggaca acgttgtata ccgtatgggc ttcggtgcca ctcgtgcaga
    13861 agcacgtcag ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg ttaacatcgc
    13921 ttcttatcag gttagtccga atgacgttgt aagcattcgt gagaaagcga agaagcagtc
    13981 tcgcgtgaaa gccgctctgg agctggctga gcagcgtgaa aagccaacct ggctggaagt
    14041 tgatgctggc aagatggaag gtacgtttaa gcgtaagccg gagcgttctg atctgtctgc
    14101 ggacattaac gaacacctga tcgtcgagct ttactccaag taaagcttag taccaaagag
    14161 aggacacaat gcagggttct gtgacagagt ttctaaaacc gcgcctggtt gatatcgagc
    14221 aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc tttggccata
    14281 ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat gccgggttgc gcggtgaccg
    14341 aggttgagat tgatggtgta ctacatgagt acagcaccaa agaaggcgtt caggaagata
    14401 tcctggaaat cctgctcaac ctgaaagggc tggcggtgag agttcagggc aaagatgaag
    14461 ttattcttac cttgaataaa tctggcattg gccctgtgac tgcagccgat atcacccacg
    14521 acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc gatgagaacg
    14581 cgtctattag catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg gcttctaccc
    14641 gaattcattc ggaagaagat gagcgcccaa tcggccgtct gctggtcgac gcatgctaca
    14701 gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag cgtaccgacc
    14761 tggacaagct ggtcatcgaa atggaaacca acggcacaat cgatcctgaa gaggcgattc
    14821 gtcgtgcggc aaccattctg gctgaacaac tggaagcttt cgttgactta cgtgatgtac
    14881 gtcagcctga agtgaaagaa gagaaaccag agttcgatcc gatcctgctg cgccctgttg
    14941 acgatctgga attgactgtc cgctctgcta actgccttaa agcagaagct atccactata
    15001 tcggtgatct ggtacagcgt accgaggttg agctccttaa aacgcctaac cttggtaaaa
    15061 aatctcttac tgagattaaa gacgtgctgg cttcccgtgg actgtctctg ggcatgcgcc
    15121 tggaaaactg gccaccggca agcatcgctg acgagtaacc ggatcacagg ttaaggtttt
    15181 actgagaagg ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa ccgcaacagc
    15241 agccatcgcc aggctatgtt ccgcaatatg gcaggttcac tggttcgtca tgaaatcatc
    15301 aagacgactc tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct gattactctt
    15361 gccaagactg atagcgttgc taatcgtcgt ctggcattcg cccgtactcg tgataacgag
    15421 atcgtggcaa aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc cggtggttac
    15481 actcgtattc tgaagtgtgg cttccgtgca ggcgacaacg cgccgatggc ttacatcgag
    15541 ctggttgatc gttcagagaa agcagaagct gctgcagagt aatctgaagc aacgtaaaaa
    15601 aacccgcccc ggcgggtttt tttatacccg tagcatcccc acttatctac aatagctgta
    15661 ctctttttgt tcatcccctg gagtatttat gtggttactt gaccagtggg cagagcgcca
    15721 tatagcagaa gcgcaagcga aaggtgagtt tgataacctg gcaggtagcg gcgaaccatt
    15781 gatactggat gatgattctc atgtgccacc ggaattacgt gcggggtatc gcttgctgaa
    15841 gaatgccggt tgcttaccgc cagaacttga gcaacggagg gaagcaatcc agcttctgga
    15901 tatcctcaaa ggtatccgtc acgatgatcc gcaatatcaa gaggttagcc gtcgattgtc
    15961 attactggaa ttgaagctgc gacaagctgg attgagtacc gattttttac gcggcgatta
    16021 tgctgacaag ttgttgaata aaatcaacga taactagtgg agtatgtatg tatcgcattg
    16081 gtgagctggc aaaaatggcg gaagtaacac ccgacacgat tcgttattac gaaaagcagc
    16141 agatgatgga gcatgaagtg cgtaccgaag gtgggtttcg cctgtatacc gaaagcgatc
    16201 tccagcgatt gaaatttatc cgccatgcca gacaactagg tttcagtctg gagtcgatcc
    16261 gcgagttgct gtcgatccgt atcgatcctg aacaccatac ctgtcaggag tcaaaaggca
    16321 ttgtgcagga aagattgcag gaagtcgaag cacggatagc cgagttgcag agtatgcagc
    16381 gttccttgca acgccttaac gatgcctgtt gtgggaccgc ccatagcagt gtttattgct
    16441 cgattcttga agctcttgaa caaggggcga gtggcgttaa gagtggctgt tgattttttg
    16501 cactggcagg attacactca cgccgttaaa taaccaactg gagtttttat gagccgatat
    16561 cagcatacta aagggcagat aaaggataat gcgatagaag cattactaca tgatccctta
    16621 ttccgacagc gcgtagagaa aaataagaag gggaaaggca gttacatgcg aaaaggaaaa
    16681 catggcaatc gggggaactg ggaggccagt ggcaagaaag taaatcactt ttttaccact
    16741 ggtcttctgc tttcaggggc ttgttaagag cggttattct gctctttcag caaatcacga
    16801 atttctgtca gtaatacttc ttctttagtt ggtgctggtg cggctgctgg ttcttctttt
    16861 ttccgattca gtttgttgat tagcttaatc gccataaaga tggcaaaggc cacaatcaga
    16921 aaatcaaaga cgttttgaat gaagacaccg taatgcatca caacagcagg gatatccccc
    16981 tgcgcatcgc gtagcgtgac agcaaactgt ttaaagtcga tcccgccaat taataagccc
    17041 agtggtggca tgatgatatc ggcaaccagt gaagatacaa tcttcccgaa tgccgcaccg
    17101 ataatgacac ccaccgccaa atccaccacg ttcccgcgca tcgcaaattc gcgaaattct
    17161 ttaataatgc tcatgttatt ctccctatga agccgacaac cataagtcta acaaatgtta
    17221 agccattttc ctgccaggaa tgaatttaat taatccttga taatcaaagg gaaataataa
    17281 ggcgtcatta gacgccttat taattacaag aagaaagggc ttggctggaa gagtctttcg
    17341 acgtcggtaa taaatttttt atctgtgagg aacataatta cgtgatcgcc ttgctcaatg
    17401 cgcagattgt cattggcaat catcacgtcg tttccacgta ccaccgctcc aataatcgtt
    17461 cctggcggta gcttgatttc gtcaatgact ctgccgacaa cgcgtgaggt gctttcatca
    17521 ccgtgagcaa cggcttcaat agcttctgct acgccgcggc gcaatgagga aacaccaaca
    17581 atatctgctt ttcgcacatg gctaagcaac gcagaaatag ttgcttgttg tggtgaaatc
    17641 gcaatatcga tcacgctccc ctgaaccaga tccacataag cgcgacgctg gatcaatacc
    17701 atcacctttt tcgcacccat acgtttggca agcatggcgg acatgatatt ggcctcgtca
    17761 tcgttggtga cggcaataaa cagatcaact tgatcgatat gttcttcggc cagcagttct
    17821 tgatccgacg catcaccaaa aaagacgatc gtattctgca acttttccgc cagttcggca
    17881 gcgcgctgct gattacgttc gatgagttta acgctgtaat ctttttccag acgacgcgcc
    17941 agccctgcac cgatattacc gccaccaacc agcatgatcc gcttatacgg tttttccagt
    18001 cgctgtaatt cactcatcac cgcgcggata tgctgtgaag cggcaataaa gaacacttca
    18061 tcaccagcct caacaatggt cgaaccttgc ggacgaatgg ggcgatcgtg gcggaaaatt
    18121 gctgccacac gagtatcgat atgtggcata tgttcgcgca tggtcgaaag tgcattacca
    18181 atcagcgggc cgccataata ggctttaacc acagcgaggc tgactttacc ctcagcgaag
    18241 ttcaccacct gcaatgcgcc gggatactca atcagtcggt aaatattatc gataaccaac
    18301 tgctctggtg cgatcagatg atcaatcggt acagcatctg aatgaaatag cttatcggca
    18361 tcgcgtacgt agtctggtga gcggatacga gcgatgcgat taggggtgtt gaaaagtgag
    18421 taggctacct ggcaggcaac catattggtt tcatctgaac tggttacagc aaccagcata
    18481 tcggcgtcgt cggcacctgc ctcccgcaat acgcgtggat gagagccatg cccctgcacg
    18541 actcgcaggt caaatttatc ctgcaaggtt cgcagacgct caccgttggt atcgacaaca
    18601 gtgatatcgt tgttctcgcc aaccaggttt tccgccagtg tgccgccaac ctggccggca
    18661 cccagaatga taattttcat cagtcgcgac ccgttatctc atcacttttt gattagctta
    18721 gcgtaaaaga agccgtcgcc ctcttcggca ccaggcagat tttgtttacc cggttgctct
    18781 ggtgttcctg tttcgcaaag ttcggcatca gcggtacgtt gcaaaaaggc tttaatctgc
    18841 aggctattct cttccggtaa caccgaacag gtggcataga ccagggttcc accggatttt
    18901 aaatgcggcc aaatggcgtc gagaatttca gactgcaatt gcgcgagttc tgggatatcg
    18961 cgatcgcgac gtaaccattt aatatccgga tggcgacgaa tcacaccggt tgctgaacaa
    19021 ggcgcatcta ataaaatgcg atcaaactgt tgctcgccac accattggga agggtaacgg
    19081 ccatcacctt gtttcacggt tgccttcata ccaaggcgtt ttaaattgtc gtaaacgcga
    19141 gagaggcgct gttcgtcaat atcaaccgca acaacctgcg cttctggtgc cacttcaagg
    19201 atatgcgttg ttttaccgcc gggggcggca caaagatcca gaatgtgttc accgttttgt
    19261 ggcgcaagcc aggtcatgca accttgtgct gatgcatcct gaacggtaac ccatccgtct
    19321 tcaaaaccag gtagcgcatg aacaggtgca ggtgtttcca gccgtacagc atcagggtaa
    19381 tccgcatgcg ggaaaccttt cattcctgct tcatccagca atgcaagcca ggtgtcgcgg
    19441 gaatgatgcg tacgattaat acgcagccac attggcggac gctggttatt ggcttcgacg
    19501 atggattgcc actgctctgg atacgctttt tgcagacgct tcagcaacca ggaaggatgc
    19561 agataacgtg catcactggt attaaactcg gctaataact cttcttgctg acgctggaac
    19621 tggcgtaata caccgttaat caacccttta agttgcggac gcttaattgc gatagcgcct
    19681 tcaaccgttt cagccagcgc agcatgaggt ggaatgcggg tataaagcag ttgatacaaa
    19741 ccaaccataa tcaggtaatg cacggtccgc tgtttgccgg tcatcggacg ggccattgac
    19801 ttattaatca gccagtctaa ctgcgaaagc gtacgcagta cgccaaagca caactcttga
    19861 agaagtgctt tgtctttatc ggaaactttt tgctggagcg gtggcagaat gttgcttaat
    19921 gattgccctt gctcgacgac ttgttcaacg gcctgggccg ccatgctacg taaattacgt
    19981 tgttttttca taagtataaa aatgcccggc aagaccgggc ttagaagagt ggactatcag
    20041 accagacggt tgcccggaac aaaccattcc cgacgagagt tcaggaggtc ttgcgcgctc
    20101 atcgctttct tacccgcagg ttgtaacgag agcaggttca ggatgccatc accagtcgca
    20161 acctgaatgc cttgtttgtt ggcttcaagg atcgttcctg gtgcagcgtt ggttgccgta
    20221 tcaatgaccg atgctttcca gactttaacc ggctgtcctt caatttccac ccagctcatt
    20281 ggccatggat tgaaagcgcg aatgcagcgt tcaagctgtg ctgccgaaag tgaccagtca
    20341 atacgcgctt cttctttact caacttctcg gcgtaagtga caagagtttc gtcctgaact
    20401 tctggtttcg ccgtgccgtc tgccaattgt ttcaacgtgg tgataagccc ttgtgggcca
    20461 agctctgcca gcttgtcgta cagcgtacca ctggtatctt ctgcagtaat cgggcaggag
    20521 agcttataga gcatgtcacc ggtgtctaaa ccaacatcca tttgcataat ggtcacacca
    20581 gtttctgcat cacccgccca tagtgagcgt tggattggtg cagcaccgcg ccagcgtggc
    20641 agcagtgaac catgaacgtt gatacagcca agacgcggca tctccagcac tgctttaggc
    20701 agaattaagc cataggcgac gacaaccata acatcagcct gtagatcggc gaccagttgc
    20761 tggttttctt gtggacgcag ggaaacaggt tgaaaaacgg gcagaccttt ttcctcagcc
    20821 agaactttaa ccgggctggg catcagtttt ttaccgcgtc ctgccggtcg gtctggctgg
    20881 gtgaacacgc caacgacgtt atgaccagaa gacaacagcg cgtcgagatg acgcgctgca
    20941 aagtcaggtg tacccgcaaa aataatacgt agtgattctg acacgttagt tcttatcctt
    21001 aagcccgggc tttcagacga tccagttttt caactttctg acgaatacgt tgttgtttca
    21061 gcggtgacag ataatccata aacagtttgc cgaccaggtg atccatctca tgctgaatac
    21121 agatggctaa cagaccatct gcctccagtt caaatggttt accgtcgcgg tccagggcgc
    21181 gaattttaac tttctctgcg cgtggcacta aagcacgttg ttcagggatc gacaggcaac
    21241 cttcttcaat gcctgtttcg ccgctttttt ctaaaagctc cggattgatt aacaccagcc
    21301 gttcgtcacg gttttccgaa acatcaataa caatgatacg ttgatggata tcaacctggg
    21361 ttgccgccag gccaatacct tcttctgcgt acatcgtctc gaacatatca tcgacgatac
    21421 gctgaatttc tgcattcact tcttctaccg gtttagcaac tttgcgaagc cgctcgtccg
    21481 gaatatgtaa cacttgcaaa actgacataa atctccagag atgtgttcag gagttagaaa
    21541 gattatttct tctattctag acaaatcccc ctctgattga cagcatcact gaccaatcgc
    21601 aaagattgct aaggctgctt atggcaggga gataaggatg gtcgatacag aaatttggct
    21661 gcgtttaatg agtatcagca gcttgtacgg cgatgatatg gtccgtatag ctcactggct
    21721 ggcaagacag tcgcatattg atgcggttgt attgcagcaa acagggctta cattgcggca
    21781 ggcacaacgc tttctttcat ttccgcggaa gagtatcgaa agctcacttt gttggttgga
    21841 gcaacccaac catcatttaa tccctgcgga cagcgaattt tatcctcctc aacttctggc
    21901 gacgacagat taccccggtg cactgtttgt tgaaggagaa ctgcacgcgc tgcattcatt
    21961 tcagcttgcc gtagtgggga gtcgggcgca ttcatggtat ggcgagcgat ggggacgatt
    22021 attttgcgaa actctggcga agcatggagt gacaattacg agtggactgg cgcgtggaat
    22081 cgatggtgta gcgcataaag cagccttaca ggtaaatggc gtcagcattg ctgtattggg
    22141 gaatggactt aataccattc atccccgccg tcatgcccga ctggctgcca gtctgcttga
    22201 acaggggggc gctctcgtct cggaatttcc cctcgatgtt ccaccccttg cttacaattt
    22261 cccacgaaga aatcgcatta tcagtggtct aagtaaaggt gtactggtgg tggaagcggc
    22321 tttgcgtagt ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc gagaagtttt
    22381 tgccttgcca ggtccaatag ggaatccggg aagcgaaggg cctcactggt taataaaaca
    22441 aggtgcgatt cttgtgacgg aaccggaaga aattctggaa aacttgcaat ttggattgca
    22501 ctggttgcca gacgcccctg aaaattcatt ttattcacca gatcaggaag acgtggcatt
    22561 gccatttcct gagctcctgg ctaacgtagg agatgaggta acacctgttg acgtcgtcgc
    22621 tgaacgtgcc ggccaacctg tgccagaggt agttactcaa ctactcgaac tggagttagc
    22681 aggatggatc gcagctgtac ccggcggcta tgtccgattg aggagggcat gccatgttcg
    22741 acgtactaat gtatttgttt gaaacctata ttcacacaga agctgagttg cgtgtggatc
    22801 aagacaaact tgaacaggat cttaccgacg caggatttga tcgagaagat atctacaatg
    22861 ccctgctttg gctggaaaag cttgcggatt atcaggaagg actggcagaa ccgatgcaac
    22921 tggcctctga tccactctcc atgcgtattt atacccctga ggagtgtgaa agactggatg
    22981 ccagctgtcg tggttttctg cttttccttg agcaaattca ggtgcttaac cttgaaaccc
    23041 gtgaaatggt gatagagcga gtgctcgcgc tggataccgc agagttcgac ctggaagacc
    23101 tgaaatgggt aatcctgatg gtgttgttca atattccggg ctgcgaaaat gcgtaccagc
    23161 aaatggaaga attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat
    23221 tcagcataag ttgttatggc gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc
    23281 ccaaagtgcg gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc
    23341 tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatc
    23401 gtcaaagttc tggaggggca ggtttgccct gcatgtggcg caaatctggt attacgccag
    23461 ggacgctttg gtatgtttat tggttgcatt aactaccctg aatgcgaaca taccgaactt
    23521 atcgataaac cggacgaaac agcaattaca tgtccccaat gccggacggg ccatctggtc
    23581 cagcgccgct cccgttatgg caaaacattt cactcctgtg atcgctaccc ggagtgtcaa
    23641 tttgccatta acttcaaacc catagctgga gaatgccctg agtgtcatta tccgctactc
    23701 atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag
    23761 ccggtttcgg cggaataata acgtgaataa taacctgcaa ggagacgcta tcgcagctgc
    23821 gatagatgtt ctcaatgaag aacgtgtcat cgcctatcca acggaagccg ttttcggtgt
    23881 tgggtgcgat cctgatagcg aaacagcagt gatgcgactg ttggagttaa aacagcgtcc
    23941 ggttgataag gggctgattt taatcgcagc aaattacgag cagcttaaac cctatattga
    24001 tgacaccatg ttgactgacg tgcagcgtga aaccattttt tcccgctggc caggtcctgt
    24061 cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc
    24121 gcttgctgta cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa
    24181 accgctggtt tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga
    24241 agttcgcgca caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt
    24301 aaatccttca gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa
    24361 tggaaaccta tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc
    24421 agcaatttgc tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca
    24481 atgatttcgt caacacactg aacgctttct ttagtgctgg tggtaaaggt gcgaatgtga
    24541 cggtgccttt taaagaagag gcttttgcca gagcggatga gcttactgaa cgcgcagcgt
    24601 tggctggtgc tgttaacacc ctcaagcggt tagaagatgg acgcttgctg ggtgacaata
    24661 ccgatggtgt aggcttgtta agcgatctgg aacgtctgtc ttttatccgt cctggtttac
    24721 gtattctgct catcggcgca ggtggagcat ctcgcggcgt actactgcca cttctttccc
    24781 tggactgtgc ggtgacaatc actaatcgga cggtatcccg agcggaagag ttggctaaat
    24841 tgtttgcgca cactggcagt attcaggcgt tgggtatgga cgaactggaa ggtcatgagt
    24901 ttgatctcat tattaatgcc acatccagtg gcatcagtgg tgatattccg gcgatcccgt
    24961 catcgctcat tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc
    25021 cttttttggc atggtgtgag cagcgaggct caaagcctac tgctgatggt ttaggaatgc
    25081 tggtggcaca ggcggctcat gccttccttc tttggcatgg cgttctgcct gatgtagaac
    25141 cagttataaa gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga
    25201 cagagaagag tgggtcgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggcat
    25261 gcaactgaca tgtgcgatct ctggcgatag cctggcgtat cgctttacag gagatacgcc
    25321 agagcagtgg ttagcgagtt ttcgtcagca tcgctgggac ttggaagaag aagcggaaaa
    25381 cttaattcag gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata
    25441 ttcgtccttc catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc
    25501 acttaacggg cggatctgtt tgacgggact accgagatac agatatccgc tctccagccg
    25561 tttattttgt gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc
    25621 atcaagtaaa attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag
    25681 catcaccttg tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt
    25741 gtacgaggat ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc
    25801 gatctgtaca taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc
    25861 agccagacga acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat
    25921 ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa
    25981 tgatttacta atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa
    26041 aagcaccgtt ttgtgtgcga atgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa
    26101 gatcaaaaaa gtacttgtgc aaaaaattgg gatccctata atgcgcctcc gttgagacga
    26161 caacgtgaaa cacttcacaa gatggtcgta acaacgaaga gaaaaaatcc tg
//
LOCUS       DAZFKY010000054        25978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFKY010000054 DAZFKY010000000
VERSION     DAZFKY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..878
                     /locus_tag="ACHKCL_001972"
     CDS             3..878
                     /locus_tag="ACHKCL_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5762943.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            986..3022
                     /gene="yihQ"
                     /locus_tag="ACHKCL_001973"
     CDS             986..3022
                     /gene="yihQ"
                     /locus_tag="ACHKCL_001973"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO5762944.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            3068..4453
                     /locus_tag="ACHKCL_001974"
     CDS             3068..4453
                     /locus_tag="ACHKCL_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762945.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            4496..5899
                     /locus_tag="ACHKCL_001975"
     CDS             4496..5899
                     /locus_tag="ACHKCL_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762946.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5966..6658
                     /gene="ompL"
                     /locus_tag="ACHKCL_001976"
     CDS             5966..6658
                     /gene="ompL"
                     /locus_tag="ACHKCL_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO5762947.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(6749..8014)
                     /locus_tag="ACHKCL_001977"
     CDS             complement(6749..8014)
                     /locus_tag="ACHKCL_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5762948.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(8116..9096)
                     /locus_tag="ACHKCL_001978"
     CDS             complement(8116..9096)
                     /locus_tag="ACHKCL_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5762949.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(9104..9814)
                     /locus_tag="ACHKCL_001979"
     CDS             complement(9104..9814)
                     /locus_tag="ACHKCL_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5762950.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(10031..11854)
                     /gene="typA"
                     /locus_tag="ACHKCL_001980"
     CDS             complement(10031..11854)
                     /gene="typA"
                     /locus_tag="ACHKCL_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5762951.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            12227..13636
                     /gene="glnA"
                     /locus_tag="ACHKCL_001981"
     CDS             12227..13636
                     /gene="glnA"
                     /locus_tag="ACHKCL_001981"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5762952.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13810..14859
                     /gene="glnL"
                     /locus_tag="ACHKCL_001982"
     CDS             13810..14859
                     /gene="glnL"
                     /locus_tag="ACHKCL_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5762953.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14871..16280
                     /gene="glnG"
                     /locus_tag="ACHKCL_001983"
     CDS             14871..16280
                     /gene="glnG"
                     /locus_tag="ACHKCL_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5762954.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            16392..16502
                     /locus_tag="ACHKCL_001984"
     CDS             16392..16502
                     /locus_tag="ACHKCL_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5762955.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(16692..18065)
                     /gene="hemN"
                     /locus_tag="ACHKCL_001985"
     CDS             complement(16692..18065)
                     /gene="hemN"
                     /locus_tag="ACHKCL_001985"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5762956.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(18254..18760)
                     /gene="yihI"
                     /locus_tag="ACHKCL_001986"
     CDS             complement(18254..18760)
                     /gene="yihI"
                     /locus_tag="ACHKCL_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5762957.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(18879..19045)
                     /locus_tag="ACHKCL_001987"
                     /pseudo
     CDS             complement(18879..19045)
                     /locus_tag="ACHKCL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19342..19974
                     /gene="yihA"
                     /locus_tag="ACHKCL_001988"
     CDS             19342..19974
                     /gene="yihA"
                     /locus_tag="ACHKCL_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5762958.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(20356..23142)
                     /gene="polA"
                     /locus_tag="ACHKCL_001989"
     CDS             complement(20356..23142)
                     /gene="polA"
                     /locus_tag="ACHKCL_001989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5762959.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            23506..24438
                     /locus_tag="ACHKCL_001990"
     CDS             23506..24438
                     /locus_tag="ACHKCL_001990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5762960.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(24479..25909)
                     /locus_tag="ACHKCL_001991"
     CDS             complement(24479..25909)
                     /locus_tag="ACHKCL_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5762961.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         6284 a         6514 c         6280 g         6900 t
ORIGIN      
        1 ttatggactt ttttacgtta ttttcaccgg atggggtcat gctggaaaca aacgtggtat
       61 gtatcgttat cgcgttaatg tttctttata cctttgtggg gatatgcgcg ggatttggcg
      121 gtggtttaac cactatgccg ctcgtcacgt taatgctccc cgtaaaaatg gcaacaccac
      181 tctcggtaat tgtcggcacc gcgaccgcct tgtacgccac ctggctgtca cgtaaagaaa
      241 cggattggcg atcggcaatg gtactgatta ttttctcgtt tctgggaata cccgtcggtt
      301 tatatgcact gtcgtattta cccgaccatt ttatgaaagt gggcttaggc ggattcctta
      361 tcctgtactc cttttacagt atgtttattc cacgcctgcc gatttatgac cgtagctgga
      421 tcgccgcacc tatcggtgca gtagcaggcg cattaggcgc agctttctca accaacggac
      481 caccgatggt tatctacggt atgctgcgta acttaggccc ggcggcattt cgtggcactc
      541 tgaacgcttt cttcaccgca aacaacatcg cggtgatcgg cgggctggcg accagcggaa
      601 ttttgactgt ttcgatcttt aaactggtgc tgttttgtat tcccaccatg atcctcggtt
      661 cactggttgg acaatatgtg cataaacgta tttcagtcaa agtgttccgt gtgatggtct
      721 ttttattgct gattgcctcc ggttccatgt tgataaaagg cgcgctggga attcccgccg
      781 ctgccgcact tctacctccg tttaccttgt tagttatact gcaactggta tttggtaaaa
      841 aagtggcggc aatccgtcag gctgaagcaa gtaaataatc acttatgcgg gtttaaattt
      901 atatccgcac acattcttta attaattaaa cctgtaataa caggatggca tgaaatatta
      961 gctttatact ctatggagat tatttatgga tacgccacgc ccacaattac cagattttga
     1021 atttcatcag aataacgaca gttttaccct acattttcga caacgtctta ttttaaccca
     1081 tagcaaagat aatccttgtt tatggattgg ctcaggtata gcggacatcg atatgttccg
     1141 cggcaatttc agcattaaag ataaactaca ggagaaaatt gcgcttaccg acgccatcgt
     1201 cagccagtca ccggatggtt ggttaatcca tttcagtcgt ggttctgaca ttagcgccac
     1261 gctgaatatc tctgccgacg atcagggccg tttattgctg gaactacaaa acgacaacct
     1321 taaccacaac cgcatctggc tgcgccttgc cgctcaacca gaggaccata tctacggctg
     1381 cggcgaacag ttttcctact tcgatctgcg cggcaaaccg ttcccgctat ggaccagtga
     1441 acaaggcgtt ggtcgcaaca aacaaaccta tgtcacctgg caggccgact gcaaagaaaa
     1501 tgcgggcggc gactattact ggactttctt cccacagcct acgtttgtca gcacgcagaa
     1561 gtattattgc catgttgata acagttgcta tatgaacttc gactttagtg ccccggaata
     1621 ccatgaactg gcgctgtggg aagacaaagc aacgctgcgt tttgaatgtg ctgacacata
     1681 catctccctg ctggaaaaat taaccgccct gctgggacgc cagccagaac tgcccgactg
     1741 gatttatgac ggagtaacgc tcggcattca gggcgggacg gaagtgtgcc agaagaaact
     1801 ggacaccatg cgtaacgcgg gcgtgaaggt caacggcatc tgggcgcagg actggtccgg
     1861 tattcgtatg acctcttttg gcaaacgcgt gatgtggaac tggaagtgga acagcgaaaa
     1921 ctacccgcaa ctggattcgc gtatcaagca gtggaacaaa gagggcgtgc agttccttgc
     1981 ctatatcaac ccgtatgttg ccagtgataa agatctctgc gaagaggcgg caaagcgcgg
     2041 ttatctggca aaagatgtcg ccggtggcga ctatctggtg gagtttggcg agttctacgg
     2101 cggcgttgtc gatctcacca atccggaagc ctacgcctgg ttcaaggaag ttatcaaaaa
     2161 gaacatgatt gaactcggct gcggcggctg gatggctgac ttcggcgagt atctgcccac
     2221 cgacacgtac ctgcataacg gcatcagcgc ggaaattatg cataacgcct ggcccgcgct
     2281 gtgggcgaaa tgtaactacg aagcccttga agaaacgggc aagctcggcg agatcctctt
     2341 ctttatgcgc gccggttcta ccggtagcca gaaatactcc accatgatgt gggcgggtga
     2401 ccagaacgtg gactggagtc tggacgatgg cctggcgtcg gttgtcccgg cggcgctatc
     2461 tctggcaatg accggacatg gcctgcatca cagcgacatt ggcggttata ccaccctgtt
     2521 tgagatgaag cgcagcaaag agctgctgct gcgctggtgc gatttcagcg ccttcacgcc
     2581 gatgatgcgc acccacgaag gtaaccgtcc tggcgacaac tggcagtttg acggcgacgc
     2641 ggaaaccatc gcccatttcg cccgtatgac caccgtcttc accaccctga aaccttacct
     2701 gaaagaggcc gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc cgctgttcct
     2761 gcattacgaa gacgacgcgc agacctattc cctgaaatat cagtacctgt taggtcgcga
     2821 cattctggtt gctccggtac acgaagaagg tcgcagcgac tggacgctct atctgccaga
     2881 ggataactgg gtccacgcct ggaccggaga aaccttccac ggtggagaaa taactgttga
     2941 agcgcccatc ggcaagccgc cggtctttta tcgcgccgac agcgaatggg ctgcactgtt
     3001 cgcctcgtta aaaaacatct aatcatgccc gcccgtgggg aaactcacgg gcaaatggag
     3061 aataacgatg agtcacatca caacggaaga tccggcaact ttgcgcctgc cctttaaaga
     3121 gaaactctct tacggtatcg gcgatctggc ctctaacatc ctgctggata ttggtacgct
     3181 ttatcttttg aagttttata ccgacgttct ggggctacct ggcacctatg gcggcattat
     3241 ctttttgatc tcgaaattct ttaccgcctt taccgatatg ggaaccggca tcatgttgga
     3301 ttcccggcgt aagattggcc cgaaaggtaa gttccgccct ttcattttgt acgcgtcatt
     3361 cccggtcacc ttattggcaa tcgctaactt tatcggcaca ccgttcgatg tcactggtaa
     3421 aacggtgatg gccactattc tgtttatgct ctacgggctg tttttcagca tgatgaactg
     3481 ctcctatggc gcgatggtgc ctgctattac caaaaacccc aacgagcgcg catcactggc
     3541 ggcatggcgt cagggaggcg ctacgctggg cctgctgctg tgcacggtgg gattcgtgcc
     3601 ggttatgaat cttatcgaag gtaatcagca acttggctat atcttcgccg ccacgctgtt
     3661 ttcactgttc ggcctgctgt ttatgtggat ctgctactcg ggcgtgaaag agcgttatgt
     3721 cgaaacccaa ccagccaatc cggcgcaaaa gcctggcctg ctgcaatctt tccgcgcaat
     3781 tgccggtaac cgcccactgt tcattctgtg cattgccaac ctctgcactt taggggcgtt
     3841 taacgtcaag ctcgccatcc aggtctatta cacccagtac gtacttaacg atcccatcct
     3901 gttgtcatat atgggatttt tcagcatggg ctgtattttc atcggtgtgt tcctgatgcc
     3961 tggcgcagtc aggcgttttg gtaagaagaa ggtctatatc ggcggcctac tgatttgggt
     4021 gctgggcgat ctgctcaact atttcttcag cggcggttcg gtcagcttcg tggcgttctc
     4081 ctgcctggca ttcttcggct cagcgtttgt taacagcctg aactgggcgc tggtttccga
     4141 caccgtcgag tacggcgagt ggcgtaccgg cgttcgttcg gaaggaacgg tctacaccgg
     4201 cttcaccttc tttcgcaaag tgtctcaggc gctggctggt ttcttccccg gctggatgct
     4261 gacgcaaatc ggttatgtgc cgaacgtggc gcaggctgac cacaccattg aagggttgcg
     4321 ccagctgatc ttcatctacc caagcgcact ggcggtagtc accattgtgg cgatgggctg
     4381 cttctacagc ctgaacgaga agatgtatgt ccgcattgtt gaagaaatag aagcccgtaa
     4441 acgcacggcg taataataat aaacaatgcc ctgcggggcg ttataaggag tgattatgtc
     4501 tgaccataat ccactgacat taaaactgaa tctgcgggaa aaaatcgcct atggtatggg
     4561 cgacgttggt tcgaatttaa tgcttagcat cggtactctg tatctcctca aattttatac
     4621 cgatgaatta gggatgccag cttactatgg cgggattatc tttctggtgg cgaagttttt
     4681 taccgcgttt accgatatgc tcaccggatt tttactcgat tcgcggaaaa atattgggcc
     4741 taaaggcaaa tttcgtccct ttattttata cgcagcggtt ccggcggcgt taattgcaac
     4801 gctccagttt atcgccacca cttttagttt gccggttaaa acgacgattg ccaccgcgct
     4861 gtttatgatg tttggacttt catacagtct gatgaactgc tcgtatggtg cgatgatccc
     4921 ggccattact aaaaacccga atgagcgcgc gcagctcgcg gcttaccgcc agggtggtgc
     4981 aaccataggg ctattgattt gtaccgtggc gtttattccg ttgcagtcgc ttttttctga
     5041 ctcaaccgtc ggttatgcct gtgcggcact gatgttctcc attggtggct ttatttttat
     5101 gatgctgtgc tacagaggcg tcaaagagca ttatgtggac acagcaccaa ccggacataa
     5161 agccagtatt ctcaaatctt tttgcgcgat atttcggaat ccgccattgc tggttttatg
     5221 cattgctaac ttgtgtaccc tggcggcatt taatatcaaa ctggcgattc aggtctatta
     5281 cacccagtat gtgctgaatg atattaattt attgtcgtgg atgggattct tcagtatggg
     5341 atgcatcctc gtcggcgtat tactggtgcc attaactgta aaatgttttg gtaaaaaaca
     5401 ggtttattta gctggcatgg tgctatgggc ggttggtgat atcctgaatt atttctgggg
     5461 aagtaactct ttcactttcg tcatgttctc ttgtatcgcc ttttttggca cggcctttgt
     5521 aaacagcctg aactgggcac tggtgccaga taccgtagat tacggtgaat ggaaaaccgg
     5581 tattcgtgcc gaaggttctg tttataccgg ttataccttc tttcgtaaaa tttctgccgc
     5641 acttgctggc ttcttgccag gcattatgct gacgcaaatc ggttatgttc ccaacatcgc
     5701 gcaaagtgat gcgacattac agggtttgcg tcagctcatt tttatctggc cttgtgcact
     5761 ggcaattatt gctgcattga cgatgggatt cttttacaca ctcaatgaaa aacggtttgc
     5821 gttgattatt gaggaaatca accaacgcaa aaataaagag atggcgacag aagaaaaaac
     5881 ggcttccgta acgttataaa tgtcaatagc cgctatttcc atcctggtgg gtggcggcct
     5941 ccctacgttt aaaaatggac ttattatgaa aaatattaat gcaataattt tattatcttc
     6001 tctgacctct gcctcagtat tcgctggcgc atacgtcgaa aacagggagg catataatct
     6061 cgcctccgat cagggcgagg ttatgctacg tgtgggttat aacttcgata tgggcgcggg
     6121 tattatgtta accaatacct acacctttca gcgagaagat gaactaaaac atggatataa
     6181 cgaaatcgaa ggttggtatc cgttatttaa accaaccgat aaattaacca tccagcccgg
     6241 tggcttaatt aatgataaga gtatcggttc gggtggtgct gtttatctgg atgttaacta
     6301 caaatttact ccgtggttta acctgacagt gcgcaaccgt tttaaccata ataactacag
     6361 ctcaaccgat ttaaacggtg atctggataa taatgacagc tacgagattg gcaactactg
     6421 gaactttatt attaccgata agttctcata tacctttgag ccgcattatt tttataacat
     6481 taatgatttc aatagcggca atggcacgaa gcatcactgg gaaatcacta acaccttccg
     6541 ttaccgcatt aacgagcact ggctgcccta tattgaattg cgttggttag atcgcaacgt
     6601 ggaaccgtac caccgcgagc aaaaccagat tcgtatcggg acgaagtatt tcttctgatg
     6661 tgaaactgcc ggatgcggcg taaacgcctt atccggccta cggttgatac ggttttcgtt
     6721 ggccggatta agtacatccg gccattaatt aagcttcagc cgccagcatc tctttcttac
     6781 gttggctaat acgcttaacc agcattacgg aaaccaccgc accacagaat gcgaagcagg
     6841 ccataatgcc aaacacgatt ttgtagccaa taatccccgg atttaaatcc agaatgtagc
     6901 catacagact gaagcagaac atcgccgggg cgtaaccaat aaagctaccc aacgccatcg
     6961 ccgcgcctgt tttattttca gcaatttttg cttcgccgat aggtgcaaaa aatacggctc
     7021 gctgtgtaaa gactatcgcg ccaaagccca gcgtacatgc catccctaaa tagaccggca
     7081 tactttcgtg cggcagcata atcaacagta cgagcgcagc ggtactgatg ataaaggtgt
     7141 agcatagata tttactcggc gatttcagga ttttatctga aatcatgcca ccaatcggtc
     7201 caccaatcat tttcagacag tattggttaa tgatgccgta agcccccacc agcgcaacgg
     7261 gcaatagata gatgtttttc aggaatggaa tgaagaatgt caggccacag taaaccgcgt
     7321 aaacgaagaa gacgttgaaa gcgataagcc agatagtttt gtctttcagc accgaggtca
     7381 tactggtgtt ttgcgatgcc ccgtcttctt ttttcacctc aacggacggt gcctcttctt
     7441 tgtcattcag gacaaagaaa atagtaatgc ctacggcaat cacaataagg gaatagaacc
     7501 agatgcctgc tttaaaacct aataagccac tgccaaacca ggtaaatact gccaacgcag
     7561 aaaatgccac cacggtatcg acaatgccac gccctgtttc gaagaagcca aacaaccgac
     7621 cttgttgttc gctattgccc aatcgactta ccgacttgag caagaccggc cagttcatca
     7681 tgtcgcaagt aacgccaaat agcgcccaga caaagaggat gccccaatac cccggcattg
     7741 tcgtcaaata aacacccagt aatcctgtcg caatgagtga gaatgacatg gtgtatctgc
     7801 gcggtagttt atcggcaaaa taaatagaca gaaaaaagcc cactgtggta acaaatgagt
     7861 ttaccgacat agcattacca atttgaccat tggtcaaatg gaaatattcc tgcatcggga
     7921 tataaaacgc atctttcagc gacggtaatt tataaattgt accgccacac agtgttaata
     7981 gactaaataa cgcccatttc tttttcgtga gcataacatc ccctaagcga ttttaaaaat
     8041 cactgtttta aaggcaataa gataccgacc attcttatgg aatggtttta ttaactgcgt
     8101 atgagttaaa acttgttagt ggttcagaag atggctcgcc tcttgtttga tttgttgtag
     8161 tacgttacgc acatgattta tctgattaat tgcatcttct ttttctttac gtaaccgctc
     8221 atccagcaaa tgcttttctg gcaatggtgt ttcactcatc tggcgataag ggatccacgg
     8281 attatcatct tcgtcttcaa agcggaacgc tggagcataa taatcaacct cttcttccag
     8341 gccatatgcc gtcacctgcg gttcttcatc gccgaggcag ataaggtgtg ttaataactc
     8401 tttgaacggc atatctccct gacctgaaat acaggcttta tgacccaggc caccttgttc
     8461 tttaacgatc aaggcatctt tgatatggac ctgggtaata tgcggtgcca tcgtttttaa
     8521 ggcgtctatg ggatgctcat ttgcattgat catattcgca aaatcaaaca gtaaggaaag
     8581 agactccatc tcactttctt tgaccagcga caccaactca tgacttttta aatcttcatg
     8641 ctgttcgatc gtaaaagtca ggccgctatc ctgatacgct tcccgcacat aggcaatgtc
     8701 gttagcgatg atcgataata cgtcgcgcaa attaccttca taacgtggat aaaaacgtac
     8761 ggacgatgcc ccagttttca acgcgatggc gacggcttcg tcaatagatg ccttatctga
     8821 ggcgctggtt tcaatatgaa tatcaaggtt cagacggcgg gctttatccc caaaggcaga
     8881 gagttcttta tcgtccatat taccaagaga aaaacgctcg ccatccagaa catggatttt
     8941 gacgccacgt agattatttt cacttgcgat atcaagtaaa tcagcaggca acactctttc
     9001 cagccgcata tttaaatgaa aggcataagc atgtaaataa agaggaagag tgtcgacacg
     9061 ttgtagaatc tttcttgcgt tatttattgt caccataaaa cacctacaga ttaatgatga
     9121 ggtctacggc ctcaaatcgc caatattcaa tatctatctg gacaatattg ccttgctggt
     9181 cggcgcgatg cttttcaatt aagatcgctg gcatacccgc agaaccgcca agatacttgc
     9241 tgatatcgcc cggcattctg acgggtttga aaatcaattt ttttactacc gtctcttttt
     9301 ggtagcactt ttcccagact gcagaaaatg agtggttttc aagttgttca ataaaacccg
     9361 gagcaacttc aggattaata tatgtttcgt gatagaaaac tttatgtcct tccagcgccc
     9421 cccagccggt gatgcgatag agttcagttg atggtgtcac tgcaatcagt ggcgcgagcg
     9481 tctcgggaat atcgctggta cggtttttct cggtaaaccc ccaggaaggc tctcgccctt
     9541 gctcaattgc ggcccgctga aagctcgccg acagctccgg actgtaatta aatcgcggct
     9601 gggtcacgaa ccacccctta cgatccttac ggaagatttt ggattctgcc tcgaggttca
     9661 acaacgcctg gcgcagcgtc atacgtttaa tgcccagcag ttctcccagc tctctttctg
     9721 aagggagttt tccacccggc gtggtgatgc catctttcaa ccaccgatcc agtttctctt
     9781 ttgcattttc tacggtggat tgattctccg ccattgttta atccttttgg tttaaaccag
     9841 atgaagcatc ttacgcttac gccaaaaaag aaagcaatta acggcgtatg actttgcaga
     9901 taattgtgac aaagctctct attgacgtaa ccacagactt ataagggaga tactcgcatt
     9961 ggtgtgcgct cacgacaggc aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta
    10021 aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcgcg acgacgatcg ttttccgtca
    10081 ggtgacgttt acgaatacgg atagaggtcg gagtcacttc taccagttcg tcatcatcga
    10141 tgaactccag agcttgttcc agagtcatgc ggataggcgg aaccagaaca acggcttcgt
    10201 cagtaccgga agcacgcatg ttggtcagtt tcttaccggt caggcagttt acagtcaggt
    10261 cgttagagcg gctatgaata ccgataatct gaccttcgta aacttctgca ccgtgaccga
    10321 ggaacagctt accgcgatcc tgcaaaccga acagcgcgaa cgcgaccgct ttaccctgac
    10381 cgttagagat cagtacgccg ttctgacgct gacccacttc acccggacgc acgtcgtcgt
    10441 agtggctgaa ggtggagtac agcagacccg taccggaagt catggtcatg aactcagaac
    10501 ggaagccaat cagaccacgg cttgggatca cgtagtcgag acgtacgcgg cctttaccgt
    10561 ctggattcat gtttttcagg tcgcctttac gttcgcccag cgcctgcatt acagaaccct
    10621 gatgctgttc ttcaacgtcc agagtcacgt tttcatacgg ctcttgttta cgaccgtcga
    10681 tttcacggaa gataactttc ggacgggata ccgccagttc gaaaccttca cgacgcatgt
    10741 tttcgatcag aacagacagg tgcagttcgc cacgaccaga aacgcggaac gcatcggcgt
    10801 cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag ttctttgttc agacgatcca
    10861 ggatctgacg agacgttacg aacttacctt ctttaccgca gaacggcgag gtgttaacgc
    10921 agaagaacat agaaacggtc ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt
    10981 gcgtgtcgca aacggtgtca gaaatgttca gttcgcccag gcccgtgatc gccacgatat
    11041 cgccagcttc cgccagatcg gtttcgatac gttccagacc gaggtggccc agcactttac
    11101 caactttcgc gttgcgggtt ttgccttcgc tatcgatgat agtaacctgc tggttcggct
    11161 tcactttacc gcgcttgatg cggccaatgc cgataacgcc aacatagctg ttgtaatcga
    11221 gctgagaaat ctgcatctgg aacggaccgt caaggtcaac gtccggcgca ggaacgtggt
    11281 caacaatcgc ctggtacagc ggggtcatgt cttccgccat atcttcgtgg tccagacccg
    11341 cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt
    11401 cgaggttaac gaacagatcg aatacctgat ccacaaccca atcaggacgc gcgccagggc
    11461 ggtcaacttt gttgataaca acaatcggct tcaggccgta agcaaacgct tttttggtta
    11521 cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac caccagcagc actgagtcta
    11581 ccatggacat tacacgttca acttcaccac cgaagtcggc gtgccccggg gtatcaacga
    11641 tgttgatacg gtaatcattc catttgatag cggtgttttt cgcgaggatg gtaatcccac
    11701 gctctttctc caaatcgttg gagtccatca cgcgctcttg ggtttcggca cgagagtcga
    11761 acgtaccgga ttgttggagc agcttgtcta ccagggtggt tttaccatgg tctacgtgcg
    11821 cgatgatggc gatattacgc aatttttcga tcacaacttt gcctcaggca ttagaaatag
    11881 cgcgttattg tacacggatt aatcgcacta caaaacagga tcacaaacat cctccgcaaa
    11941 caagtattgc agagtccctt tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag
    12001 gtcattgcac caatatggtg cttaatgttt ccattgaagc actatattgg tgcaacattc
    12061 acatcatggt gcagcccttt tgcacgatgt taagcatgat aacgcctttt aggggcaatt
    12121 taaaagttgg cacagttttc gctttatctt ttttacggcg acacggccaa aataattgca
    12181 gatttcgtta ccacgacgac aatgaccaat ccaggagagt taaagtatgt ccgctgaaca
    12241 cgtactgacg atgctgaacg agcacgaagt gaagtttgtt gatttgcgct tcaccgatac
    12301 caaaggtaaa gaacagcacg tcactatccc tgctcatcag gtgaatgctg aattcttcga
    12361 agaaggcaaa atgtttgacg gctcctcgat tggcggctgg aaaggcatta acgaatccga
    12421 catggtgctg atgccagacg catccaccgc agtgattgac ccgttcttcg ccgactccac
    12481 cctgattatc cgttgcgaca tccttgaacc tggcaccctg caaggctatg accgtgaccc
    12541 gcgctctatt gcgaagcgcg ccgaagatta cctgcgttcc actggcattg ccgacaccgt
    12601 actgttcggg ccagaacctg aattcttcct gttcgatgac atccgtttcg gatcatctat
    12661 ctccggttcc cacgttgcta tcgacgatat cgaaggcgca tggaactcct ccacccaata
    12721 cgaaggtggt aacaaaggtc accgtccggc agtgaaaggc ggttacttcc cggttccgcc
    12781 ggtagactct gctcaggata ttcgttctga aatgtgtctg gtgatggaac agatgggtct
    12841 ggtggttgaa gcccatcacc acgaagtagc gactgctggt cagaacgaag tggctacccg
    12901 cttcaatacc atgaccaaaa aagctgacga aattcagatc tacaaatatg ttgtgcacaa
    12961 cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa
    13021 cggctccggt atgcactgcc acatgtcgct gtctaaaaac ggcgttaacc tgttcgcagg
    13081 cgacaaatac gcaggtctgt ctgagcaggc gctgtactac attggcggcg taatcaaaca
    13141 cgctaaagcg attaacgccc tggcaaaccc gaccaccaac tcttataagc gtctggtccc
    13201 gggctacgaa gctccggtaa tgctggctta ctctgcacgc aaccgttctg cgtctatccg
    13261 tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa gtacgtttcc cggatccggc
    13321 ggctaacccg tacctgtgct ttgctgccct gctgatggcc ggtcttgatg gcatcaagaa
    13381 caagatccat ccgggcgaag ccatggacaa aaacctgtat gacctgccgc cggaagaagc
    13441 gaaagagatc ccacaggttg caggctctct ggaagaagca ctgaacgagc tggatctgga
    13501 ccgcgagttc ctgaaagccg gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc
    13561 tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg catccggtag agtttgagct
    13621 gtactacagc gtctaatagt tgaagttgta ctacccggcg caacaacgcc gggatttagt
    13681 tgccgtggaa actttcagcc catctctgca tgggcttttt tctccgtcaa ttctctgatg
    13741 cttcgcgctt tttatccgta aaaagctata atgcactaaa atggtgcaac ctgttcagga
    13801 gactgcttta tggcaacagg cacgcagccc gatgctgggc agatcctcaa ctcgctgatt
    13861 aacagtattt tgttaatcga tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa
    13921 caactgctcg cccaaagctc ccgcaaattg tttggtacgc cgttaccgga actgttgagc
    13981 tacttctcat taaatattga gctgatgcaa gaaagtctgg aggcggggca aggttttacc
    14041 gataacgaag tgacgctggt catcgacggt cgctcgcata tcctttctgt gacggcccag
    14101 cgtatgccgg acggcatgat cctgctggag atggcgccga tggataacca gcgccgctta
    14161 agccaggaac agctacaaca tgctcaacaa gttgctgccc gtgatttagt gcgtggcctg
    14221 gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct actcagcaaa
    14281 gcattacctg acccgtcact gctggaatat accaaagtga ttatcgaaca ggcggaccgg
    14341 ctgcgaaatc tggtcgaccg tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa
    14401 agtattcaca aagtagctga acgcgtggta acgctggtgt cgatggaact gccggacaac
    14461 gtgcggttga ttcgtgatta cgatcccagc ctgccggaac tggcgcacga cccggatcaa
    14521 attgaacagg tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg gccggaaggc
    14581 ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga ccttacacgg cgaacgctat
    14641 cggctggcgg cgcggattga tgtggaagat aacgggccag gcattccgcc tcatttgcag
    14701 gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc
    14761 atcgctcgta atttgattga tcagcattca ggcaaaattg aatttaccag ttggccaggg
    14821 cataccgagt tctcggttta cctgcctatc aggaaataaa ggtgacgttt atgcaacgag
    14881 ggatagtctg ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg
    14941 ctggagcggg tttaacctgt acaacatttg agaacggcgc ggaggtactg gaggcgctgg
    15001 cgagcaaaac gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg
    15061 cactgctcaa gcagattaaa cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac
    15121 attccgatct ggatgctgcc gtcagcgcct atcaacaagg ggcgtttgat tatctgccca
    15181 aaccgtttga tatcgacgaa gccgtggcgc tggttgagcg cgccatcagt cattaccagg
    15241 aacagcagca gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggcgaag
    15301 cgccagccat gcaggacgtg ttccgtatta tcggtcggct ttcgcgttct tctattagcg
    15361 tgctgattaa cggcgaatcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc
    15421 acagtccgcg agccaaagcg ccatttatcg ctctgaatat ggcggctatc ccgaaggatt
    15481 tgatcgaatc agaactgttt ggtcacgaga aaggcgcgtt taccggcgcg aataccattc
    15541 gtcaggggcg ttttgaacag gctgatggcg gtacattatt tctcgatgaa attggcgata
    15601 tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg
    15661 ttggcggcta tgcgccggtg aaagtggatg tgcggattat cgctgccact caccagaatc
    15721 tcgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta
    15781 tccgcgttca tctgccgccg ctgcgtgagc gtcgggaaga tattccccgc ctggcgcgcc
    15841 attttttaca ggttgccgcg cgagaactgg gcgtagaagc gaagttgctg catccggaaa
    15901 ccgaagccgc gttgacgcgt ctggcgtggc caggcaacgt gcgccagctg gaaaacacct
    15961 gccgctggct aacggtaatg gctgccggac aggaagtgtt gattcaggat ttgcctggcg
    16021 aactgtttga atcaacggtt gcggagagta cttcgcaaat gcaaccggac agttgggcga
    16081 cgcttcttgc gcagtgggca gacagagcgc tacgttccgg tcatcaaaat ctgctttccg
    16141 aagcgcagcc agagctggag cggacgttac tgacgaccgc gttgcgacat acgcaggggc
    16201 ataaacagga ggcggcgcgg ctactcggct ggggccgcaa caccctgacg cgtaagttaa
    16261 aagagctggg gatggagtga ttcacggctt gtgtgtaaag attgattatt gagcgcaaat
    16321 tgctggtatt ttgcgcttta ctgttccgat aagttcagta tgatcttgcc cggaaaacgg
    16381 ggagagtcat tatgctggaa tcaataatta atctggtatc gagtggcgca gttgacagcc
    16441 acacaccgca aactgctgtt gctgccgtgc tgtgtgccgc gatgattggg ctgtttagtt
    16501 gagtttcaga ttgctgatga agtgctcttt gttcatgcca gatgctgcgt gaacgcctta
    16561 tccggcctac aaaagcaata ttttcaacag gttttgtagg cctgataagc gtagcgcatc
    16621 aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt aaacgaagcg
    16681 ccattcacta tttaaatcac ccgagagaac tgctgcatcc gcgctttctg gcgcagatag
    16741 gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt cacctgtatt
    16801 cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag cttgagatct
    16861 tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct caataggggc gtaatccaga
    16921 cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc
    16981 gcaataccac gccacagcgc attgccttgt tcatccactt gctgatagta ctgcttcaac
    17041 tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac gcccatcccc
    17101 agcagatcgg tatcgccctg agtggtgtag ccctggaaat tacgatgcag cacgccttca
    17161 cgctgggcca ccgccagctc gtcatccgga cgggcaaagt gatccatacc gataaactga
    17221 tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga
    17281 ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg cagatgcgca
    17341 tagttaaaga cgctcagacg gtcagggttt agctccgcca cgcgtttcag ggtaaaggcg
    17401 aaactttccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt ggaggtaaag
    17461 ccaatctcac gcgcatggtt aagcagcgca aagataaact cttcatcctg ctcgcggtta
    17521 accagacgtt gcacttcttt gttgaagtcc tgcacgccca tactcaggcg attaaagccc
    17581 tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac ttcgatcgaa
    17641 atctccgcat cggcattgaa ctggaagttt tcgcgcagca gcttcatcag gcggctgatt
    17701 tgcgctttat taagatacgt cggcgttccg ccgccccagt gcagctggct gacgtgacgc
    17761 ccggcgaaca gcggtgcacg atggacgatt tcttgctcca gcgcgtccag atactgatcg
    17821 gccttgtgct gctggcgagt aacaatctta ttgcagccgc agaagtaaca aagcttatgg
    17881 cagaacggga tatgtacgta gagagataat ggacgctcag gatagcgcgc cacggcttgt
    17941 aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta cgaggtgtat
    18001 cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat ttgctgtaca
    18061 gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt
    18121 ttcgggtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa
    18181 cgaataacca caccagataa catataaccg gaagggttat cagcagaacg ggaaggccca
    18241 cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt
    18301 cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac tcatcaatac
    18361 gatccagttt agcatccacc caggattgct cttcggcact cagggtttcg cctgcttcca
    18421 gacgttccag cagcgcatcc agacgctcat ccgtttctag taattccaac tccgcctgcg
    18481 gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc
    18541 ccaatggaat aggggtttta ctgccaatac gtggatcttt cggtgcgttc tggcctttgc
    18601 tgcctgacgt gttgtttccg cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt
    18661 tctgacgctt gcggtcacga gcttcctgat tcagctcttc gcgcgttttg cgacgcgctt
    18721 ttgcatgacc tttgctgcgt gatggtgaag atggtttcat aatggttcgt cttttagccg
    18781 ttttattcag tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga
    18841 cagattactc tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg
    18901 caggccgcca acacaccctg tttttccatt agccggaaca atccgtgttg attccatttc
    18961 cgtttaatta cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc
    19021 gctcctgacc ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac
    19081 tttacgcctt tgctttaaac aaacaatcaa ccgccatcag tatttgcaca ttttcagagt
    19141 tttatataaa agcaattaac tgatttatat agcttatttg acatgcatcg cagaattttc
    19201 tctgctattt cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc ttttacttac
    19261 aacgctcacg ccgaaaaatc cgccttttat tatggttcag gtataatcgc cgcaaagctt
    19321 tgactgacgg agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg
    19381 agtgcgcctg atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc
    19441 cgttccaacg caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct
    19501 cgtacctcaa aaacccctgg acgcacccag cttatcaacc tgtttgaagt ggctgacggc
    19561 aagcgtctgg ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc
    19621 aaatggcagc gcgcgctcgg cgagtacctc gaaaaacgtc agagcctaca aggtctggta
    19681 gtgctaatgg atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg
    19741 gtagacagca atatcgccgt gctggtactg ctgaccaaag cggacaaact ggcaagcggc
    19801 gcacgtaaag cgcaattgaa tatggtgcgt gaagcggtgc tggcgtttaa cggtgatgtg
    19861 caggttgaaa cgttttcttc gctgaagaaa caaggcgtgg acaagctgcg gcagaaactg
    19921 gatacctggt ttaacgagat gcagcctgta gaagaaacgc aggacggcga ataattttct
    19981 taccttaatg cttatgccgg atgtggcgta tccggcccat aaattcaata gattacaatt
    20041 ctaccgttgg cctgataaac atggtgcatc aggcattacg gatctttcct ttcgcccaat
    20101 aaaaaacgcc ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac
    20161 gtgaagtaaa aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt
    20221 actctttttt gttcagttca gaaagcactt tttgtaattt tttttcagtt gttgcatagg
    20281 aatttctaat agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc
    20341 atgttcaggc gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag
    20401 caacggcaca tccagacggg tacagttttc catcagttga tgaatctgct tcgcaacggc
    20461 atcgacatca tctctatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg
    20521 tacacgcggt tgctcagcct gtaaccacgc atcaacggca atcatcgccc gtttgataat
    20581 gtcggcggcg gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg
    20641 agcaccattg ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac
    20701 gtagccctgc tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta
    20761 gcgttcgaag taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg
    20821 cgccagaccg aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg
    20881 gcgttgctcg ctggtgacgg tttccagtgg caaaccaaac acttctgccg ccgttgcccg
    20941 gtggatatct tttccttccg cgaatgcggt cagcaagcct ttgtcacgcg agagatgcgc
    21001 cataatgcgc agttcaatct gcgagtagtc cgctgagaca atcacataat cctctggcgc
    21061 aataaacgcc tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt
    21121 aggatcggtt gacgataaac gtcccgttgc agttacagcc tggtgataag aggtatgcac
    21181 acgcccggtt ttcgggttga tcatcagcgg cagcttgtcg gtgtaagtcg atttcagctt
    21241 cgccagacca cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc
    21301 cagtacctct tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaatgcc
    21361 ctgtttttca aacagaatgg tttgtaactg cttggtggaa gaaaggttaa attcctcacc
    21421 tgcaatttca tgcgctttct tttccagctc agccagacga agggtgagct cttcagaatg
    21481 attgtgcagc actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagtactgg
    21541 caccagcggc atttcgatat tctcgaagac gttcaacggc cctttgtgtt tttgcagatc
    21601 cggccacatt ttcagatgca actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc
    21661 ggcctcttcg agggcaatct ggttaaaagt cagttgattt ttgcctttac ccgcaatctc
    21721 ttcaaaagtg atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg
    21781 cccggcaacg ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag
    21841 ctcaatgccg taattcgcca gaataccgcg atcgtatttc aggttttgcc cgaccttcag
    21901 cgccttttca tcttccagca gcggttttag caactcgagt gcacgctcgc gagagatttg
    21961 atcgggcgca tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat
    22021 agcaaaagaa agcccgacca ggttagctga gatgttatca aggctgtcgg tttcggtatc
    22081 aaatgcaaat accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc
    22141 aaggatggtg acgtagttgt cataagaaat caccgttgcc gtcacttctg gtgcttcgtc
    22201 tgcaacactg gtttcctgtg gcttcgcggc tggttttgcc cctttggcct gtaaccattt
    22261 gcccgcttcg acatcagcag tccagcgttt gaactcatac tttttgaaca gccccaacaa
    22321 ctcttccgct gccggttgct gcacttccag ttgttcacag gtcagctcca gttcaacgtc
    22381 ggttttaatc gtcgccagct ggtatgagag ataagcaact tctttgtttt gctcgagctt
    22441 cgctgccatt gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata
    22501 cagcgtatcc agcccgccaa gaccttgcag caatgcctgc gcggtttttt caccgacgcc
    22561 cggtacgcca ggaatgttat cagaggagtc acccatcagc gccaggaaat cgatgatcag
    22621 ttctggcggc acgccgtact tattcaccac ctcttccggt ccgaggatgg tattcgtcat
    22681 ggtgttgata agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgccagtgct
    22741 gatcagcacc ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc
    22801 cgcttctacg ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcaa
    22861 gggttcgatt tgtgctcgca gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc
    22921 aaacagttca tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg
    22981 tttatattgc atgatcagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg
    23041 ctcgcctgcg ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga
    23101 accatctaca aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta
    23161 tcagattatg ggtaaaggat gccacagaca ggataaaaac atgagttttt caccgctttt
    23221 cgtgaaaatc ttttgtagat cttctggatc gctcgcaaat cgtcatgtgg ataactttgt
    23281 gcataaatta tttacatcgc ttttaattta ttatcatgct ttttacagca tgatgtttgt
    23341 ctttgttttt catgcagtta aaaataaatc catccctaat aattgagata atgggatttt
    23401 ttctaatgtg gatagatatg aattattttt ctccttaagg atcatccgtt atttgggtcg
    23461 ttttctgcta aaatctgcgc cttgcgcttc tttagcgcac tgtctatggc taaccttttg
    23521 aataaattca ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg
    23581 accattttgg tcactatttt ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta
    23641 ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat
    23701 tgctggtgtg aaggtctggc ggtattactg catctgaacc cacacctgca atgggaagtt
    23761 cacgggctgg aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt
    23821 tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat
    23881 ttcctcaagc aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat
    23941 atgccgttta tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt
    24001 aaagatgttg aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt
    24061 gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact
    24121 ttccaaaact tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt
    24181 aaacaattcg ataaactgct taatgttacg ttgtgttatc cggacaataa ccgtcagccg
    24241 ttcttcgata tgttaagcgg taaactgacg cggattgtcg tacatgtgga tttacagcct
    24301 attgccgatg agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag
    24361 caatggctga actcattgtg gcaggaaaaa gaccgacttc tgacgtcgtt gatgtcatca
    24421 cagcggcagg acaagtaaat tttgctcgcc tctgaatgag cagaggcgag tgagtatttt
    24481 aatgaacaaa acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa
    24541 aaacaacttt acccggtgta taacgcagct gcagcgaggc ggtattgttg tcaatcgtga
    24601 ttaactgaaa catctgacct aatgtcatca tcccgctgat ttgtttatca gcctgctttt
    24661 gcgctttttc cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga
    24721 cattaagcgg taacttcaca tcaaaattga gcgatttgat atctttgttt gtggatgatg
    24781 aagatttggc tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt
    24841 tcttccatct taccggttgt ttaatggtcg gctcactttg ttgtaataac ggtaaatact
    24901 ctttgaacaa tgcagcattc acttcttgca gagcaacttc atcgtttgct aattcagggt
    24961 gtgcagcaag ctgcttctgc atcgcaatgt tatactggat gataaattgg cgcaccgctg
    25021 agggatcaat agattcagca atcaatgacc actcaccact accgagttgc tgattccctt
    25081 ttgttaactt atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc
    25141 gatccagcct ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga
    25201 taatgttaat ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta
    25261 atcgcgccag agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt
    25321 gcagaacaat atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctactatcac
    25381 cattaaaagt aaactggccc tcaccaaaag aaaactttcc atactctgca ggggcaacat
    25441 ctaatgtcgt agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt
    25501 tatttttcgc ggcgataaat agcggttgtg tcagttcatt attcactaag ttcacttttg
    25561 ccgctgccag tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt
    25621 gctcaacatc cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac
    25681 cgttatcgaa tgtcatttgc atttgaaaac gggaattaaa aaagcctttg tcaaaatttt
    25741 gataacttaa cgtcatatcg taggcatgtt cacctttctt tttagcatcg ttaaaatctt
    25801 taataaattt ttcgacacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc
    25861 agattacggc taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc
    25921 ctgataaaac agcaaaactt tgaatatcca cttatgctga aataatgtcc agcggcag
//
LOCUS       DAZFKY010000055        25317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFKY010000055 DAZFKY010000000
VERSION     DAZFKY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..1743
                     /gene="yhgE"
                     /locus_tag="ACHKCL_001992"
     CDS             19..1743
                     /gene="yhgE"
                     /locus_tag="ACHKCL_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5762962.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(1806..2684)
                     /gene="hslO"
                     /locus_tag="ACHKCL_001993"
     CDS             complement(1806..2684)
                     /gene="hslO"
                     /locus_tag="ACHKCL_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5762963.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(2709..3110)
                     /gene="hslR"
                     /locus_tag="ACHKCL_001994"
     CDS             complement(2709..3110)
                     /gene="hslR"
                     /locus_tag="ACHKCL_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5762964.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(3121..3789)
                     /gene="yrfG"
                     /locus_tag="ACHKCL_001995"
     CDS             complement(3121..3789)
                     /gene="yrfG"
                     /locus_tag="ACHKCL_001995"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5762965.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(3854..5989)
                     /gene="igaA"
                     /locus_tag="ACHKCL_001996"
     CDS             complement(3854..5989)
                     /gene="igaA"
                     /locus_tag="ACHKCL_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5762966.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            6309..6869
                     /gene="nudE"
                     /locus_tag="ACHKCL_001997"
     CDS             6309..6869
                     /gene="nudE"
                     /locus_tag="ACHKCL_001997"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5762967.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(7034..9586)
                     /gene="mrcA"
                     /locus_tag="ACHKCL_001998"
     CDS             complement(7034..9586)
                     /gene="mrcA"
                     /locus_tag="ACHKCL_001998"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5762968.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            9706..10500
                     /gene="hofM"
                     /locus_tag="ACHKCL_001999"
     CDS             9706..10500
                     /gene="hofM"
                     /locus_tag="ACHKCL_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5762969.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            10484..11023
                     /gene="hofN"
                     /locus_tag="ACHKCL_002000"
     CDS             10484..11023
                     /gene="hofN"
                     /locus_tag="ACHKCL_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5762970.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            11007..11447
                     /gene="hofO"
                     /locus_tag="ACHKCL_002001"
     CDS             11007..11447
                     /gene="hofO"
                     /locus_tag="ACHKCL_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5762971.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            11437..11841
                     /gene="hofP"
                     /locus_tag="ACHKCL_002002"
     CDS             11437..11841
                     /gene="hofP"
                     /locus_tag="ACHKCL_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5762972.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            11753..12991
                     /gene="hofQ"
                     /locus_tag="ACHKCL_002003"
     CDS             11753..12991
                     /gene="hofQ"
                     /locus_tag="ACHKCL_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5762973.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            13392..13913
                     /gene="aroK"
                     /locus_tag="ACHKCL_002004"
     CDS             13392..13913
                     /gene="aroK"
                     /locus_tag="ACHKCL_002004"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5762974.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            13970..15058
                     /gene="aroB"
                     /locus_tag="ACHKCL_002005"
     CDS             13970..15058
                     /gene="aroB"
                     /locus_tag="ACHKCL_002005"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5762975.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            15150..16436
                     /gene="damX"
                     /locus_tag="ACHKCL_002006"
     CDS             15150..16436
                     /gene="damX"
                     /locus_tag="ACHKCL_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5762976.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            16543..17379
                     /gene="dam"
                     /locus_tag="ACHKCL_002007"
     CDS             16543..17379
                     /gene="dam"
                     /locus_tag="ACHKCL_002007"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5762977.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            17397..18074
                     /gene="rpe"
                     /locus_tag="ACHKCL_002008"
     CDS             17397..18074
                     /gene="rpe"
                     /locus_tag="ACHKCL_002008"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5762978.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            18067..18825
                     /gene="gph"
                     /locus_tag="ACHKCL_002009"
     CDS             18067..18825
                     /gene="gph"
                     /locus_tag="ACHKCL_002009"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5762979.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            18818..19822
                     /gene="trpS"
                     /locus_tag="ACHKCL_002010"
     CDS             18818..19822
                     /gene="trpS"
                     /locus_tag="ACHKCL_002010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5762980.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(19953..20684)
                     /gene="frlR"
                     /locus_tag="ACHKCL_002011"
                     /gene_synonym="yhfR"
     CDS             complement(19953..20684)
                     /gene="frlR"
                     /locus_tag="ACHKCL_002011"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO5762981.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(20784..21569)
                     /gene="frlD"
                     /locus_tag="ACHKCL_002012"
                     /pseudo
     CDS             complement(20784..21569)
                     /gene="frlD"
                     /locus_tag="ACHKCL_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            complement(21569..22396)
                     /gene="frlC"
                     /locus_tag="ACHKCL_002013"
     CDS             complement(21569..22396)
                     /gene="frlC"
                     /locus_tag="ACHKCL_002013"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO5762982.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            complement(22447..23469)
                     /gene="frlB"
                     /locus_tag="ACHKCL_002014"
     CDS             complement(22447..23469)
                     /gene="frlB"
                     /locus_tag="ACHKCL_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO5762983.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(23490..24827)
                     /gene="frlA"
                     /locus_tag="ACHKCL_002015"
     CDS             complement(23490..24827)
                     /gene="frlA"
                     /locus_tag="ACHKCL_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO5762984.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(25123..25290)
                     /gene="yhfL"
                     /locus_tag="ACHKCL_002016"
     CDS             complement(25123..25290)
                     /gene="yhfL"
                     /locus_tag="ACHKCL_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5762985.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         5961 a         6473 c         6873 g         6010 t
ORIGIN      
        1 tgattctgga taaccaacat ggataacgtt gaactttcac ccgcaacacg ctgggggatg
       61 attgctaccg gattacttca gggtctggtt tgctacctgc tgatcgcctg gctttccgga
      121 aaaaatcaca gttggattgt ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta
      181 ctcttctccg tgatctcttt taaacagaag cgcctttggg gatggctggc gctggtgttt
      241 attgccacgc tggggatgag cggctggctg aagtggcaaa ctgatggcat gaccccctgg
      301 agagctgaaa aggcgctttg ggattttggt tgctatctgc tgctgatggc aatgttgttg
      361 ctaccgtgga tacaacaaag ccttcgcatc cgcaatgaca gtagccgcta tcgctatttt
      421 taccagtcag tatggcataa cgtacttata ttactggtga tttttctcgc caatggtctg
      481 acgtggctgg tgcttttact gtggagtgag ttgtttaaac ttgttggcat cacgtttttc
      541 aaaacgctct tttttgcaac cgactggttt atttatctca cgttaggtct ggttaccgcg
      601 ctggcggtga tcctcgcgcg aacacagtca cgtttaatcg actctattca aaagttgttc
      661 acgttaatcg ccacggggtt gctgccgtta gtatcattgc taaccctgat gtttatcatc
      721 accctgccgt ttacaggcct gagtgcgatt tctcgccaca tctccgccgc cgggttgctg
      781 ttaacgctgg cctttttgca attgatctta atggctattg tccgcgatcc gcaaaaagcg
      841 tcacttccct ggacggggcc gttgcgttgc ctgattaaaa ccgctctgct ggttgctccg
      901 ctgtatgtgt tcatcgccgc ctgggcgtta tggctgcggg tcgctcagta cggctggact
      961 gtcgaccgct tgcagggcgc gctggcggtg ctggtgttac tggtatggtc gctggggtat
     1021 tttgtcagta tcgtctggcg taaagggcaa aatccccttg ttcttcaggg caaagtgaac
     1081 cttgcggttt cgttattggt gttggtaata ctggtgcttc ttaattcgcc ggtgctggac
     1141 agtatgcgca ttagtgtgaa cagtcatatg gcgcgttatc agagcggcaa aaacacgcca
     1201 gaccaggtaa ctatctacat gctcgaacac agcggtcgct atggacgtgc ggcgcttgag
     1261 tcgctgaaaa gcgatgccga gtatatgaaa gacccgaaac gcgcgcggga tctactgatg
     1321 gcgttagatg gagagcaaca tcttcagcaa cagatatcgg aaaaagtatt agccgaaaat
     1381 gtgttaattg cccctggttc tggtaaaccg gatgcgacat tctggtcggc cttaatccag
     1441 gatcgctata acgtgatgac ctgtattgaa aaagacgcct gcgtactggt cgagcaagat
     1501 ctgaatagtg atggcagggc agagcggatc ctctttgcct ttgatgatga aagatacatt
     1561 gtctatggct ttgatcctga caagaaagaa tggcaagagc ttacgatgag tttacttccg
     1621 cgtgatataa cgaaagaaaa attactcaca gctgcgaagg atggaaaact gggaacgaag
     1681 cctaaagcgt ggcgcgatct tgtagtggat ggtgaaaggc tggatgtgaa tctgaatgag
     1741 tgacaaagcg catcaggtgt attagtcgta aaatccggca gagctctctc tgccggacat
     1801 actcattaat gaacttgcgg atctgccgga gacgcgttgt tgcggatttc agcaatatcc
     1861 atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat ttcgccatct
     1921 tccgccagga tgctatcgac ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt
     1981 tcacgcgagc aggtgcattt gaactccaca tcctgcggat cgtaaaccgt cacctcttct
     2041 tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcagcagttc ttcggttttg
     2101 atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc
     2161 attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata
     2221 aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttccaggca ggccgccagg
     2281 gtatcacctt ccagacccac cacgccctga tagcgttcac cttcgctcgg ggtaatggtg
     2341 atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc
     2401 tgcacgcgcg ccacaccgcg catctgctgg ttgttgttac cgttaataac cgccagattc
     2461 atcggaccgt cgccttgcag ctgtacggtg atatcaccat caaacttcag cgtagcggtt
     2521 aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg cggataatcg
     2581 tgattctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc gcgcacggca
     2641 aagttttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat ctctcttgca
     2701 ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg
     2761 tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc cagcgccatt
     2821 ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgc ctcgctggcg
     2881 gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc
     2941 agagtgagcg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg cccgttgtaa
     3001 tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata aaaacgggca
     3061 gcccatagcc atttatccag tcgaacctca acagcaggtt tttctttcat ggcgtctcct
     3121 tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc gttgatactg
     3181 tttctcggca atccctgagt caggattagt cacgccgagg cagtaacgaa taccaaattg
     3241 cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt cagctttcag
     3301 acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat aaccaaatgt
     3361 gtgggtggaa agtaataaat caaggtgtgc gtccagaccg gtatgctcaa gttttaccgc
     3421 caggttgtgc ggatgcgcat tggtgagcaa aattcgctgc ttaccgctgg ctttcagtgc
     3481 ctcaagaaac ggaatggtat cttcacgcag tacggcacgc ggtcccatct cggtggtcat
     3541 cgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc agtttagcgt
     3601 atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg gcgtaacccc
     3661 gtttttcgcg ccccatgttt caggcaccag cttttgccag aaatagttat cgaaggcgag
     3721 gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc aggcaatgtt
     3781 gatatgcatg aggaaaatct ccagagtgaa gcaatttgcg cgacagggta gcataacctg
     3841 ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgc gggttcaggc
     3901 agctttcata ataggcctga atcttcatca tgcgagtgcg atgacgctgg taacgccgcc
     3961 aggcctgtac gccattataa atggcgctac ccagcatcgt cagtagcagc agcgtggtgc
     4021 tgagatagcg ccacaggccg gaacgatccg ggatcggatg aaggcctata tgctgcgtac
     4081 cattggcgtc agtgaagatt tttgtgacga taccctcggc gttaaacggc gtatgcatca
     4141 gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag tcgtaaagtg
     4201 atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca atcaggaatc
     4261 cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg
     4321 gcgcggtgga ggttgccacc aggttatcca gcgattccgc actcaccggg cgtaataaca
     4381 cattcacgcc atcgagcttc ccggcgttgg cgcgttttac cagcgcgtcc cagtctttac
     4441 tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg gcagaacaca
     4501 gatccgctgt cttcagtaca atgtcgccaa aatcatcaag caataccatg ccggattttt
     4561 gaattgctga acgtaatgag gcgctgacgc gagattcatc ttccggttta gggtgcagct
     4621 ggcgattaac tgcttcagtc aatgccgtcg ctttgttgac cagttcagat tctggtaatg
     4681 gtaatgagcg ggcgtcgttc cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat
     4741 tggttttcgc gctccaggtt ccggaagtgc gaatattaca cattcccgta ccgctaatac
     4801 gcaatgtatc gcctacccgc acgccagcgt cagccagttg ttttacgctg gtggcttcaa
     4861 tggtctgcgc acctttcatc catgagagag tgaatttcag cggcatatcc agcgggatcc
     4921 agaataacag cataaacagc accagcagcg agcccgcagc gataatcgta ctgcgcagcc
     4981 agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc ccctgacgca
     5041 ctaggtggcg gtcgagatag atatcaatat cggtttgttg accgagatcc tgagcaatgt
     5101 atggctgcca gtgtgcggga tagaccaggt cgataatacc gagtgaaata ttgttgatct
     5161 gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa
     5221 tttcccgcag ggaggatttt gccggggggg cgaatagccc ccacagacct gcgcccagca
     5281 gcagtaacgc gccgcctgcc agccacggaa caaatacatc cggggtaatc aggcagaaaa
     5341 agaacatcag gaaggaggcg acgatcagca acgcttcacg cagcccgcgc ggacgactca
     5401 gcgcatattc ttcatgcgtt tctttgcgaa tattgagtag ctcgatttgc tcactctctt
     5461 ccccgcgaat cgacgcctgc gtggacggaa caggttgcaa ggcatagccg cgagtttcct
     5521 gcatgtactc ctgcagcgta tgaccgttga gtgaaataac cagcggcagc gaatcggtat
     5581 gaatcagttc aacggtattc tcatcgttga tgtactgctc ccagaaaggg ggcaggtgga
     5641 cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag atgccgtaac
     5701 gcgtgatagc gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc gccagagaga
     5761 tcggtgccgc gctggctccc gttgggccag gcacctgtaa tacctgcgtc aggctctcaa
     5821 gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc gcatccgcga
     5881 aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc catcccgcaa
     5941 gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg ctttccccat
     6001 cttacttagt ctgtcaggcg tggtcagtcg caccggacac aatacatgat tctgcggttg
     6061 gctatcggca agcgtggagt ttaacttgag catcattaag cacatcacag taaccgctga
     6121 tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt tcaacctatt
     6181 gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta aataacatgc
     6241 aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt cacagcatag
     6301 atcccacgat gagcaaatca ttacaaaaac ccaccattct gaatgttgaa actgtagccc
     6361 gttcccgact gtttaccgtc gagagcgtgg atctagagtt cagcaatggc gtgcggcgtg
     6421 tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg attgtggacg
     6481 atcacctgat cctgatccgc gaatatgcag tgggaactga atcctacgaa ttaggttttt
     6541 cgaaaggatt aattgatccg ggtgaaagcg tcttcgaagc cgctaaccgc gagctaaaag
     6601 aagaggttgg atttggtgcg aacgatctga cttttttgaa gaagctcagc atggcaccgt
     6661 cttacttttc cagcaaaatg aatatcgtgg tagcgcaaga tctctacccg gaatcgctgg
     6721 aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat atgatggatt
     6781 tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg
     6841 aatggttgaa agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg cacaatgctt
     6901 atcaggctta tggggttcct gcaatatatt gagttttcac gattttgtta gccggataac
     6961 gcgttcaccc gtatccggca taaacaagtg cactttgtca gcaaactgaa aaggcgccga
     7021 agcgcctttt taatcagaac aattcctgtg cctcgccatt atcgataatg gtcgttccca
     7081 cctcgtgcac cgcctgttgt gtcggctgcg taccttcgat gaaatactct tcgcggctgt
     7141 tgccaccatt agctaactgc ccggtgctgc gatcgatatt caccgtcaca atacccggcg
     7201 gcggcgtcag cggctgctcc ggcacacctt caagaacggc tttcatataa gcgtcccatg
     7261 caggctgggc actcttggca ccgccttcgt aacctgaaat ctgatcttta atcgctccgg
     7321 aagccgttgt atgaccgaga ttacgacggt gatcatcaaa gccaatccag accgaggtca
     7381 caacgcccgg tccgtaaccc gagaaccacg catctttcga actgttagtg gtaccggttt
     7441 tcccgccgat atcgcgacgc tgcaaatcac gacctgcacg ccagccggta ccctgccagc
     7501 ctggctcacc aaagatattg gtgttcaaag cactcttaat caggaatgcc agcggagtgt
     7561 tgatgacgtg cggtgcgtac tcctgcgcgc cagtcttcgc cactaacgcc tgatttgcct
     7621 gctccagctg cggcattggt acagaaacat tctgctgctc gcgggagata gcgacatctt
     7681 caacatcgtt attttccagc acgttcgatt tctgcgtatc accgtaaatc accggaatat
     7741 cgcattccgg gcaggctact ttcggtttcg cttcgaaaat cacgccgccc tgatcgtttt
     7801 caattttgct gataaaccac gggtccacca ggaagccgcc gttcgccatg accgcgtagc
     7861 cgcgcgccac ctgcattggg gtgaaggacg ctgaacccag cgccagcgat tcggtgtgga
     7921 caatgttttg cgccgggaag ccgaagcgtt gcagatattc tgcagcgtag tcgacgccca
     7981 tcgcccgcat tgcgcgtacc atcaccacgt ttttcgactg acccagcccc tgacgtaagc
     8041 gaattggacc ggcatactgc ggtggtgagt ttttcggctg ccagtcagaa ccggcacctg
     8101 cgtcccagcg agaaattggc acatcgttca acatacttgc cagcgtcaga cctttatcca
     8161 tcgccgcggt gtagaggaac ggtttgatgt tggaacccac ctgacgcagt gcctgggtgg
     8221 cgcggttaaa cttgctctga ttgaaatcaa agccaccgac cagcgccata acggcaccat
     8281 tttgcggatt gatcgacacc agtgccgagt tcacttccgg cacttgtgcc agccaccatg
     8341 catcgccaac ctgacgaacc cagatttgct gacccgtttg cagaacatcg gtcactttac
     8401 gcggcgtcgg tccctgctga gtatccgaac ggtaaggacg cgcccagcga acgccttcca
     8461 tactcaatgc gacggtcgac ccgtccgcca gcattgccgt cgcttcctga ggattggcgc
     8521 tggtgactgc ggcaggcagc agcggaccat aggtcggcag cgccttcagc gtatcggtaa
     8581 tcttgttgtt atcccacgcc gactcgccca ctttccacag cacatttgcc gggccgcgat
     8641 agccgtggcg catgtcgtag tccagcacgt tattacgtac cgcctgctgc gcggcctgct
     8701 gcactttgcg ggtgatggtg gtgtaaatgc gataaccgtc ttcataggca ctttcgccat
     8761 aacggttata catctcctgg cgcaccattt cgctcaggta cggcgcagaa aaagcaatct
     8821 ccggcgcgtg atagttagcg ttaatcgcct cagtgcgtgt ctgatcgaac tgttgctggg
     8881 tgatatatcc ttcatccagc atccgcgata gcacgacgtt acgccgcgcg acggcacgat
     8941 ccatcgagta gagcgggttg aaggtggaag gcgctttcgg cagcccggct atcaccgcca
     9001 tttcgttcag cgtcagttgg tcgaccgttt ttccgaaata gacttgtgcc gcagcaccga
     9061 caccataggc gcggtaacca aggtaaatct tgttcagata aagctcgagg atctcgtctt
     9121 tcgtcagcaa ctgttcaatg cgaatcgcga ggaagacttc cttaatctta cgcatcagcg
     9181 tgcgttctgg actgaggaag aagtttctcg ccagctgctg ggtaatggta cttgcccctt
     9241 gtgacgcgtg gccggagaac agcgccacac ttgccgcacg gaagatcccc accgggtcaa
     9301 cgccgtgatg ctcgtagaag cggctgtctt ccgtcgcgat aaaggctttc accatctccg
     9361 gcgggatttg atccaacgta accggaatac gacgtttctc accgtattga gcaatcagct
     9421 cgccatcggc gctgtaaatc tgcatcggaa tttgcaggcg aacatctttt aatgtcgcca
     9481 catccggcag ttgtggctcg atgtagcggt ataggccata aatcgagcct gctcccagca
     9541 gaatgcaaca gactgcaagg atcaaaaaat actttacgaa cttcactgga aatttcccat
     9601 ttagtttcat ttgggcagtt tataaacaaa cgcgcggtag tataaaggca agccagacgc
     9661 attgatatac ccgtcagagt gacgggtgat aaggagatca tcacaatggc atttaagatc
     9721 tggcaaattg gtttgcattt acaacagcaa gaagcggtag cggttgctat tgtgcggggc
     9781 gcaaaagaat gctttttgca acgctggtgg cggttgccgc tggcgcacga cattatcaaa
     9841 gatgggcgga ttgttgatgc gcagcggctg gctaaaacgt tgttaccgtg gagtcgcgaa
     9901 ctgccgcagc gccatcacat catgttggcg tttcccgcca gtcgcacatt acagcggtca
     9961 tttccgcgcc cgtcgatgtc cctcggtgag cgggagcaaa cggcctggct gtccgggacg
    10021 atggctcgcg agctggatat ggatccggac tccctgcgct tcgattacag cgaagactca
    10081 ctcagtcccg cctataacgt gactgccgcg caaagcaaag agctggcaac gctgcttact
    10141 ttggcagaaa ggttgcgtgt tcatgttagt gcgatcaccc cggatgccag tgcattacag
    10201 cgattcctgc cttttttacc ttctcatcag caatgtctgg cctggcgtga taacgaacag
    10261 tggctgtggg cgacgcgcta tcgctggggg cgcaaactgg cggtggggat gactagcgcg
    10321 aaggagctgg cggcagcgtt atccgttgat cccgaaagcg tcgcgatatg tggcgaaggc
    10381 ggatttgatc cttgggaggc cgtttctgtt cgtcagccgc cgttaccgcc gccaggtgga
    10441 gactttgcca tcgcgctggg ctggcgcttg ggaaggcgta ctgatgaacc cgccaattaa
    10501 ttttttgccc tggcgacagc aacgccggac cgcttttctt cgtttctggt tgctgatgtt
    10561 cgttgcgcct ctgctgctgg ccgtcgggat aacgctaata ctgcgtctga caagcagcgc
    10621 cgaagctcgc gtagacgccg ttttgcttca gggggaacaa caactcgccc gcagcttaca
    10681 gataaccaaa ccacgtttgc tggagcgcca acaaattcgt gagcagcgtt tgcaaaggca
    10741 gcgccagcga caatttaccc gcgactggca atctgcgctg gaagcactgg cggcgctttt
    10801 acccgagcac gcctggctga caacgataag ctggcagcag ggaacgctgg agatcaaggg
    10861 gcttacaaca agcattaccg cgttaaacgc actagaaacg tcactccgcc aggatgcatc
    10921 ttttcatctc aatcagcggg gggccacgca gcaggatgcg cagggacgct ggcaatttga
    10981 gtatcagtta acaaggaagg ttagcgatga acatgttctt tgactggtgg ttcgccacat
    11041 caccccgcct ccgccagttt tgctgggcat tctggttgct gatgttagtt acgctcattt
    11101 ttctgtcatc gacacaccat gaagagcgcg acgcattaat tcgactacgg gcaagtcatc
    11161 atcagcattg ggccacactg tatcgcctgg ttgacaccac tcccttcagc gaagaaaaaa
    11221 cgctgccctt ttcgccactg gattttcagt tgcccggcgc acaactggtt ttctggcatc
    11281 catccgcgca gggaggcgag ttggcgttga aaacgctgtg ggaagcagtg ccgtcggcat
    11341 ttacacggct ggcagagcgc aacgtcagcg tgagccgttt ttcattaagc gtggaaggtg
    11401 atgatctttt gttcacccta caactggaga cgccgcatga gggttaaacg ctggttgttg
    11461 gcaggtattg cattgtgcct tttaaccggt atgcgtgacc cttttaaacc gccggaagat
    11521 ctatgccgga ttagcgaact tagccagtgg cgctatcagg ggatggtagg gcgaggcgag
    11581 cgcatcatcg gtgtaataaa agacgggcaa aagaaatggc gacgggtgca gcaaaacgat
    11641 gtgctggaaa acggctggac aattttacag ctgacgctag acgtactaac gctgggtacc
    11701 gggacaaact gcgaaccgcc acaatggttg tggcaacggc aaggagatac aaatgaagca
    11761 atggatagcc gcactactgt tgatgctgat acccggcgta caggcggcaa agccgcaaaa
    11821 agtgacgctg atggtggatg acgttccggt agctcaggtg ttgcaggcgc tggctgaaca
    11881 ggagaagttg aacctggtgg tgtcgccaga cgtcagcggt acggtgtcgt tacatctaac
    11941 agatgttccc tggaagcagg cactacaaac tgtagtgaaa agcgccggac tgataacgcg
    12001 ccaggagggc aacattctct cagtgcattc cattgcctgg cagaatgaca atatcgcccg
    12061 tcaggaagcg gagcaggcgc gggcgcaggc aaatctgccg ctggaaaatc gcaatattac
    12121 tctgcaatac gccgacgccg gagagctggc gaaagctggg gagaagctac tgagtgccaa
    12181 agggagtatg accgtcgata aacgcaccaa tcgccttttg ctacgagata acaaaacggc
    12241 gttaagcgcc cttgaacagt gggtagcgca aatggatctg ccggtcgggc aggttgagct
    12301 gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt gagttaggcg tgaaatggac
    12361 gctggccgat gcgcaacacg ctggtggcgt tgggcaagtc accacgcttg gtagcgacct
    12421 ctccgtagcg acggcgacaa cgcatgtcgg ttttaacatt gggcgcatca acggacgttt
    12481 gctggatctt gagctttccg cgctcgaaca aaaacagcag ctggatatta tcgccagtcc
    12541 gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa caggggagcg aaattccata
    12601 tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa tttaaagagg ccgtcctggg
    12661 gatggaggtc acgcccacgg tgttacaaaa aggtcgcatc cggctgaaat tacacatcag
    12721 ccagaacgtt ccggggcagg tgctacagca ggccgatggc gaagtgctgg cgattgataa
    12781 gcaggagatc gaaacgcagg tcgaggtcaa aagcggagaa acgttggcgc tgggcggcat
    12841 ttttacccgt aaaaataaat cgggtcagga tagcgtgccg ttgcttggcg acattccctg
    12901 gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa cgacgcgagt tagtggtgtt
    12961 tatcacgcca cgactggttt ccagtgagta aacagccgta aaagcggtaa tgtttttacg
    13021 ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag catacaagga gtaccgattt
    13081 gagagttggt gctcttcgct gcctgccttc catgatgatg atttatcatt caggcggcat
    13141 tttgctgtct tttttacgct aatcttaccc ggtgatttat cgccagagcg gtggtagcaa
    13201 ggcagcgcgc ttgcagcgac cagatatgca gagggatggg tgatttattc agttgccaaa
    13261 cccgctggag tattgagata attttcagtc tgactctcgc aatatcttat gaggtttcag
    13321 ttcatgtcct gcggagctct ctgagcgaag cgggtttatc attaacgaat agtcttagta
    13381 gtaccgaaaa aatggcagag aaacgcaata tctttctggt tgggcctatg ggtgccggaa
    13441 aaagcactat tgggcgccag ttagctcaac aactcaatat ggaattttac gattccgatc
    13501 aagagattga gaaacgaacc ggagctgatg tgggctgggt tttcgattta gaaggcgaag
    13561 aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt gaccgagaaa cagggtattg
    13621 tgctggctac tggcggcggc tctgtgaaat cccgtgaaac gcgtaaccgt ctttccgctc
    13681 gtggcgttgt cgtttatctt gaaacgacga tcgaaaagca acttgcacgc acgcagcgtg
    13741 ataaaaaacg cccgttgctg cacgttgaaa caccgccgcg tgaagttctg gaagcgttgg
    13801 ccaatgaacg caatccgctg tatgaagaga ttgccgacgt gaccattcgt actgatgatc
    13861 aaagcgctaa agtggttgca aaccagatta ttcacatgct ggaaagcaac taattctggc
    13921 tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga tgtcgcgtta tggagaggat
    13981 tgtcgttact ctcggggaac gtagttaccc aattaccatc gcatctggtt tgtttaatga
    14041 accagcttca ttcttaccgc tgaaatcggg cgagcaggtc atgttggtca ccaacgaaac
    14101 cctggctcct ctgtatctcg ataaggtccg cggcgtactt gaacaggcgg gtgttaacgt
    14161 cgatagcgtt atccttcctg acggcgagca gtataaaagc ctggctgtac tcgataccgt
    14221 ctttacggcg ttgttacaaa agccgcatgg tcgcgatact acgctggtgg cgcttggcgg
    14281 cggcgtagtg ggcgatctga ccggcttcgc ggcggcgagt tatcagcgcg gtgttcgttt
    14341 cattcaagtc ccgacgacgt tactgtcgca ggtcgattcc tccgttggcg gcaaaactgc
    14401 ggtcaaccat cccctcggta aaaacatgat tggcgcgttc taccagcctg cttcagtggt
    14461 ggtggatctc gactgtctga aaacgcttcc cccgcgtgag ttagcgtcgg ggctggcaga
    14521 agtcatcaaa tacggcatta ttcttgacgg tacgtttttt aactggctgg aagagaatct
    14581 ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac tgtattcgcc gttgttgtga
    14641 actgaaggca gaagttgtcg ccgccgacga gcgcgaaacc gggttacgtg ctttactgaa
    14701 tctgggacac acctttggtc atgccattga agctgaaatg gggtatggca attggttaca
    14761 tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg acgtcggaac gtctcgggca
    14821 gtttagttct gccgaaacgc agcgtattat aactctgctc acgcgggctg ggttaccggt
    14881 caatgggccg cgcgaaatgt ccgcgcaggc gtatttaccg catatgctgc gtgacaagaa
    14941 agtccttgcg ggagagatgc gcttaattct tccgttggca attggtaaga gtgaagttcg
    15001 cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc gattgtcaat cagcgtaaca
    15061 acaagaaagg tcaggccgct tatcaagcgg tctattagct tcaggttaat tgcaacgtgg
    15121 taagcattaa ccttttagtg gggtgttaaa tggatgaatt caaaccagaa gacgagctga
    15181 aacccgatcc cagcgatcgt cgtactggtc gttctcgtca atcttctgaa cgttctgagc
    15241 gtactgaacg tggcgaaccg cagatcaatt ttgatgatat tgaacttgat gacactgacg
    15301 atcgccgtcc gactcgtgcg caaaaagagc gcaatgagga accggaaatc gaagaagaaa
    15361 ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt agagcgtcgt ccgcgtaagc
    15421 gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat gatgatgggc gtcggcattc
    15481 tggttctact gctgttgatc atcggtatcg gttctgcgct aaaagccccc tcgaccactt
    15541 ccagcgatca aaccgcgtct ggcgagaaga gtattgatct tgctggcaat gcgaccgatc
    15601 aggcgaatgg cgtgcagcca gcgccgggaa ccacgtctgc ggaaaatact cagcaggatg
    15661 tttctctgcc accgatctct tctacgccga ctcaagggca aaccccggcg gcaacggatg
    15721 gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc gctgacccag ccacaaaatc
    15781 agcaacagtt gaacaatgtg gcggtcaatt ctacattgcc gactgaacca gcaactgtcg
    15841 cgccagtacg caatggcaat gcatcgcgtg acacggcgaa aacgcaaact gctgaacgtc
    15901 cggccactac gcgtccagct cgtcagcagg cggtgattga accgaaaaaa ccgcaagcaa
    15961 ccgtgaaaac ggaaccgaag ccggtagcac agacgccgaa gcgtactgaa ccagctgctc
    16021 ctgtggcgag cacgaaggca ccggctgcga cttctacgcc agcaccaaaa gagacggcga
    16081 ctacggctcc agtacagacg gcatccccgg cgcaaaccac ggcaacacca gccgctggag
    16141 ggaagaccgc aggtaatgtg ggttcgttga aatcggcacc gtccagccat tacactctgc
    16201 agctgagcag ttcctctaac tacgacaacc tgaacggttg ggcgaagaaa gagaatctga
    16261 aaaactacgt tgtctatgaa acgacgcgta atggtcagcc gtggtatgtc ctggtttctg
    16321 gcgtgtacgc ttcgaaagaa gaggcgaaaa aagcggtatc tacattgcca gcagatgttc
    16381 aggccaaaaa cccgtgggcg aaaccgctgc gtcaggtaca ggccgatctg aagtaatcaa
    16441 ggttatctcc cgcaatggtt tatcgttgcg ggagttgcct gaagcgctgg atgctgtcgg
    16501 agctttctcc acagccggag aaggtgtaat tagttagtca gcatgaagaa aaatcgcgct
    16561 tttttgaagt gggcaggggg caagtatccc ctgcttgatg atattaaacg gcatttgccc
    16621 aagggcgaat gtctggttga gccttttgta ggtgccgggt cggtgtttct caacaccgac
    16681 ttttctcgtt atatccttgc cgatatcaat agcgacctga tcagtctcta taacattgtg
    16741 aagatgcgta ctgatgagta cgtacaggcc gcacgcgagc tgtttgttcc cgaaacaaat
    16801 tgcgccgagg tttactatca gttccgcgaa gagttcaaca aaagccagga tccgttccgt
    16861 cgggcggtac tgtttttata tttgaaccgc tacggttaca acggcctgtg tcgttacaat
    16921 ctgcgcggtg agtttaatgt gccgtttggc cgctacaaaa aaccctattt cccggaagca
    16981 gagttgtatc acttcgctga aaaagcgcag aatgcctttt tctattgtga gtcttacgcc
    17041 gatagcatgg cgcgcgcaga tgatgcatct gtcgtctatt gcgatccacc ttatgcaccg
    17101 ctgtctgcga ccgccaactt tacggcgtat cacacaaaca gttttacgct tgaacaacaa
    17161 gcgcatctgg cggagatcgc cgaaggtctg gttgagcgcc atattcctgt gctgatctcc
    17221 aatcacgata cgatgttaac gcgtgagtgg tatcagcgcg caaaattgca tgtcgtcaaa
    17281 gttcgacgca gtataagcag caacggcggc acacgtaaaa aggtggacga actgctggct
    17341 ttgtacaaac caggagtcgt ttcacccgcg aaaaaataat tctcaaggag aagcggatga
    17401 aacagtattt gattgccccc tcaattctgt cggctgattt tgcccgcctg ggtgaagata
    17461 ccgcaaaagc cctggcagct ggcgctgatg tcgtgcattt tgacgtcatg gataaccact
    17521 acgttcccaa tctgacgatt gggccaatgg tgctgaaatc cttgcgtaac tacggcatta
    17581 ccgcccctat cgacgtacac ctgatggtga aaccggtcga tcgcattgtg cctgatttcg
    17641 ctgccgctgg tgccagcatc attacctttc atccagaagc ctccgagcat gttgaccgca
    17701 cgctgcaact gattaaagaa aatggctgta aagcgggtct ggtatttaac ccggcgacac
    17761 ctctgagcta tctggattac gtgatggata agctggatgt gatcctgctg atgtccgtca
    17821 accctggttt cggcggtcaa tctttcattc ctcaaacact ggataaactg cgcgaagtac
    17881 gtcgtcgtat cgacgagtct ggctttgaca ttcgtctgga agtggacggt ggcgtgaagg
    17941 tgaacaacat tggcgaaatc gccgcggcgg gcgcggatat gttcgttgcc ggttcggcaa
    18001 tcttcgacca gccagactac aaaaaagtca ttgatgaaat gcgcagtgaa ctggcaaagg
    18061 taagtcatga gtaagtttga agatattcgc ggcgtcgctt ttgatctcga cggtacgctg
    18121 gtggacagtg ctcccggtct tgctgctgcg gtagatatgg cgctgtatgc gctggagttg
    18181 cccgtcgcag gtgaagaacg cgttattacc tggattggta acggcgcaga tgttctgatg
    18241 gagcgcgcat tgacctgggc gcgtcaggaa cgtgcgactc tgcgtaaaac aatgggtaaa
    18301 ccgcccgttg atgacgacat tccggcagaa gaacaggtac gtattctgcg taaactgttc
    18361 gatcgttact atggcgaggt tgccgaagag gggacgtttt tgttcccgca cgttgccgat
    18421 acgttgggcg cgttgcaggc taaaggcctg ccgctaggcc tggtcaccaa caaaccgacg
    18481 ccgttcgtcg cgccgctgct cgaagcctta gatatcgcca aatacttcag cgtggtgatt
    18541 ggtggtgatg atgtgcaaaa caaaaaaccg catccggacc cgctgttact ggtggctgag
    18601 cggatgggaa ttgctccaca acagatgctg tttgttggcg actcacgcaa tgatattcag
    18661 gcagcaaaag cggcaggttg cccatcagtt ggcttaacct acggatataa ctacggcgag
    18721 gctatcgatc tcagccagcc tgatgtaatt tatcagtcta taaatgacct tctgcccgca
    18781 ttagggcttc cgcatagcga aaatcaggaa tcgaaaaatg actaagccca tcgtttttag
    18841 tggcgcacag ccctcaggtg aattgactat tggtaactac atgggtgcgc tgcgtcagtg
    18901 ggtaaacatg caggatgact accattgcat ttactgtatc gttgaccaac acgcgatcac
    18961 cgtgcgccag gatgcacaga agctgcgtaa agcgacgctg gatacgctgg ccttgtatct
    19021 ggcttgtggt atcgatcctg agaaaagcac catttttgtt cagtcccacg taccggaaca
    19081 tgcgcagtta ggctgggcac tgaactgcta tacctacttc ggcgaactga gccgcatgac
    19141 gcagtttaaa gataaatctg cgcgttatgc cgagaacatc aacgctggtc tgtttgacta
    19201 tccggtgctg atggcagcgg acatcctgct gtatcaaact aatctggtac cggtgggtga
    19261 agaccagaaa cagcacctgg aactgagccg tgatatcgcc cagcgtttca acgcgctgta
    19321 cggcgatatc tttaaggtgc cggagccgtt tattccgaaa tccggcgcgc gcgtaatgtc
    19381 gctgctggag ccgaccaaga agatgtccaa gtctgacgat aaccgcaata acgttattgg
    19441 cctgctggaa gatccgaaat cggtagtgaa gaaaatcaaa cgcgcggtca ccgactccga
    19501 cgagccgccg gtagttcgct acgatgtgca gaacaaagcg ggcgtttcca acctgctgga
    19561 tatcctttct gcggtaacgg gccagagcat cccggaactg gaaaaacagt tcgaaggcaa
    19621 gatgtatggt catctgaaag gcgaagtggc tgatgccgtt tccggtatgc tgactgaatt
    19681 gcaggaacgt taccaccgtt tccgcaacga tgaagccttc ctgcaacagg tgatgaaaga
    19741 cggcgcagaa aaagccagcg cgcacgcttc ccgtacgcta aaagcggtgt acgaagcgat
    19801 tggttttgtg gcgaagccgt aatatcaaaa cgctcggacg gtcccctcgc ccctttgggg
    19861 agagggttag ggtgagggga acgtgcggga tgtaaaattc agtgcgccgg catcgccgga
    19921 tgcgatgctg gtgcatctta tccggtctac ggttaatgtc ttttattatc aattgttaag
    19981 gtgatgcggt tggcgcggca gaagagttcg gagacgtgca ccggtttgtc attctgatca
    20041 aaggcgattt tgctgatgcg aaacaacggt tcgcccagtt cgcatttcag ccattttgcc
    20101 tgcgggcggg tggcggcaaa gatatcgatg gtctttttgt cgctgaccac tcgcgtgtca
    20161 aaacgctcct gaaataactg ataggtggag cttccttcga cgtaaatctc atcaaagtca
    20221 ggataacgcg acagcgggat ccacgaacta tcaataaata gcggctcttt atcgagatac
    20281 atcacccggc aaagatggaa cacttcgctg ttaccgggga tattgagctt ttcgcaaaac
    20341 ggcgcggcgc tgatccgttc ttgctcgatc actttctctt tcgtcgcctt gccttgtgag
    20401 acgccaaaat cggtaaaacc actgacggtc agcagggcgt tttcgacctt ctggctttgt
    20461 acaaaggtgc ctttgccctg ccagcggatc agtacgccgt ctgccactaa gtcgctgatg
    20521 gctttgcgaa tggtaatgcg gctgacgtta tattgtgtac aaagctcatt ttcggtaggg
    20581 atctgttgcc cggcctggta aaccccctgc gcgatatcat ccagcagccg ctggcggacg
    20641 gtagcgtaga ggagttgatg agagtagcgg tccgtagctg acataatcga ccctgaaata
    20701 aagaatgaat tacacggatt atagggtcgt tttccggcaa gtaacagcac gttggcgtga
    20761 agatgctcac gccaacgtta tgcctaccag gcaccgtggt actgaatagt ttgtgccgcg
    20821 cacgccgttc cctgcgccat cgcctgcggt aatgtcatcc ccgcagacca gccgcaaagg
    20881 aatccggcaa tgaacgaatc tccggcaccc atggtgtcga taaccgtcac ctgttcagga
    20941 gcctgacgcc agaactgcac gccatcccag gcaatactgc cgttttcacc caacgtgaca
    21001 atcaccacgc ctgcgccacg ggcaacaatc gctttcatct tcagacgcag cgcttcgtct
    21061 tcttgcggtg cggaggcaaa ggcaaaatcg agatgcggca ccagtgtctg ccagagcggg
    21121 ctgtcccact tgtcggagac gtcgaaagcg gtaagtttgc ccgcagcgtg cagctgtggg
    21181 aatgcgtctt ccgcatgtcc ccagattgcc gcgtgcacaa tgtcatactg cgccagctag
    21241 gcgtaatcct cttcactcag ggcaaagtcg gccatcacgc cttcggtgta gtcgccaaaa
    21301 acgcgatcat tgtcgtgcag ttccacctga gtttgtgcgg taacgccgtg tttcgtatgg
    21361 acatggctga tatcgacgcc catgctggcg agatcctgct taagctttgt gccgtagtca
    21421 tcgtcaccaa cccaggtaat gcatcccggc tttataccgt agcgagtgca gtacaccgcc
    21481 acgttcaccg cattaccgcc agaaaacgct ttattcagtt gcgggtagat atcgacgcag
    21541 ttatcgccga ttgtcgccag ggttttcatc actcatcctc cggcagcagc gcgcgaaagc
    21601 gttccagcgc ctggcgggcg taaagtctgg gctcgttcat atacatcgtt accagttcca
    21661 ctgtgcagta tccctcatag ccccgatcaa taatatcgcg catcagttcc cgcagcggca
    21721 ttttgccttc gccgggaatg taatgcgtgt cgctggcccc gtcgctgtcg acaatatgca
    21781 gatgacgtaa tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat
    21841 acggcgcgca aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat
    21901 gaagcacatc attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga
    21961 tcagatccat gccgatgttt tccgcgtact cacacagttc gcttaagttc tctgccagcc
    22021 gcccccagat aacgttaggc ggcgtgagat agcccgcgtg ggccgcagaa atcagcgtat
    22081 aacccgcgtt catctctttt gccatatcca tcgccagctt gatcatgtcc aggctttcac
    22141 gacgcatatg ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtgt
    22201 aaccgataat cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg
    22261 cttttaagtc cggcgcgaac gcgtgcgggc ggccgcccca aatttcgatg ccatcgtaac
    22321 ccagctcgct tgcatcacgg aatgcatgtt caataggcag ccgctggtgg ccgcaggtaa
    22381 acatacctgt tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag
    22441 agaggattaa tattccacca gaccaccgta atagcggcgt tcatccgggt tgtgatcttt
    22501 gtaaatagac aggtagtagc agagccactc cattggcacg aacatcagga acggtgccag
    22561 ccacgggtgc agtccttgcg aaattgcggc gtaatcgatg acgatcacgt tgtcagtacg
    22621 ctgtttaaca aagttaatgg cgcgttcggt ggtgtggcga ctttcatcat tgccgagcag
    22681 gaacaggaac ggaacgcccg gttcgacaat ctccagcggg ccgtgacgga actcaccgct
    22741 ctcaatcaca cagccgtgcg tccaggtaaa ttccatcagc gtcacgatgc cttctttgta
    22801 acccagcggg cgcagcgggc ctgcagcaac cgtgtaaatc atcggccact ggctggccag
    22861 ttcaccaagc tggcggcctt tttcttccca agtgcgtacc agataaccga gcgcattcgg
    22921 cagctgtttg agatcgtttt tgatcttgcc gatttccgcg tgcggcgcga ggcgggtgat
    22981 catctccagc accacgctgt agcagagcag cagatgaatt tcccagatac agtcggcctg
    23041 ataatcaatg ctaaattccg ccgccgaggt aatcgggcta tccgcgcgtt tggtgaacgc
    23101 cgcagtgagt gcgccgcagg cccggcccag ctccagcgct ttgattacct cttcggtttt
    23161 accgtagtca gaaacgccaa tcaccgcgca acgagcgtcg aggcgatatg gggtgttatc
    23221 gcagaactcc cagccggaaa tggcgtaaac ctgaagatcg gaaaagcgat ctgccagatg
    23281 tttcgccgtt tgcgccgcgt tgagtggcga tccgcaggca acgaaataaa tacgatcgat
    23341 gtcgcgcttc accatctctt ccacgatggt gtgcaccagc ggaacgtcat ggctgagaac
    23401 tttttccact tcctgaacca tattttcggt gaccagaaag tccacagtgc ttttatcaat
    23461 atccaacatt ttattttctc cagataacat tacgacaaag cgttgagctg gcggctacgc
    23521 ttcgcccaga aggcgtaagc aggcagacca gtggcaataa cgatgacggc gcagataagg
    23581 ccgggaatcg gtgcccagac aaaggttgag gcgaccagaa tgaggcttga cgcaatggcg
    23641 aggggggtca tcagcccgaa agccggagta cgccacagcg gtttgtaatc gtcgcgttta
    23701 cgacaccaga tgatggagcc gaaggtgagg gtatttttga aacacattac cagggtgaaa
    23761 tagcccagca ggctggtgag atcggaaacg aagatgaaga agatccccag cgccccttgc
    23821 aaaatgatgg agacatccgg cgtgttgtat ttcggatgca catggccgaa gcatttgaag
    23881 aacaggttgt ctttcgccat cgcgtattcc agccgcggct ggtacatcac gcagctggaa
    23941 agcgaaccaa gaatgacgat catcgccgtg atggcaacaa agatcccagc ggtgctgccg
    24001 agtgcgggga tccaggtcag ggcgtcggaa atcggcgttt cagaattggc gagtttgtcg
    24061 aagggcatca ggccggaaat caccagcgcc agcagggtgt agagcaccag aaccagcaga
    24121 caggaaccaa tcagcgctcg tggcatggtt ttgccggggt ttttaatttc gccggtcata
    24181 taacagatag aggccatgcc ggtatacgac caactggtgg cagagatccc cgccagcagc
    24241 gccataaagc tgcccgttgc gccaatcgca gtggtagcag gggcggcaaa attctccgct
    24301 ttaaaccaga agatcccaag accaatgacg atagtgaacg ggataatttt ggcgatggta
    24361 attagcgtct gaaacgctgc gccgccttca acggagcgca ggtgcagcag cataaaggcg
    24421 ataattaatc cggcggcgat aaatttaccg agcaacggat cgataggcgt taaaaagcca
    24481 agattgctga caatcgccag cgccataatc gacaatgacg gcgcatcgtt ggcccagaag
    24541 ctggcccagc cggagaggaa agccagcggt cggcttccgg catttttcag ataaacataa
    24601 tctgcgccat tttccggata agcggtggat agttccgcat atacgcacat ttgcgggatc
    24661 acaattaacc cgccgatgac aaatgcgagc accgtcagcc acggcgtgcc cgccgctttt
    24721 gccacttcac ccacagatac aaaaataccg gagccgacgg ttgtcccgac ggcgattgca
    24781 agaacggccc aaaatccgag cttgcgttgg agttcctggc ttcccatagc gatacctttt
    24841 tcttgtccaa cacgttgtag gggagagttt gcagtcattt aaaaggtgac atgacaatgc
    24901 atgatgaata taacaacata tgatgttatg taatgtggag gaagatcaca taatttcgca
    24961 caggatcgcg ctgtggctga tgaatgtggt tatccaattg aaattaaagt gaaaatattt
    25021 ttacgggtgg ggagcaagaa ggatatagaa acaaatacgc cctcgggtaa tcccgagggc
    25081 gtcaggcaat taaatcagcg ttagctgatt tctgtcagag acttactgtg cagtaggacc
    25141 gcaaccgcca atgattttag aaatagaaat tgctgggtgc agcaggtagt cgtaagagca
    25201 gttcttatca cggttttcaa tatgaccagt acatgcagtt aacgtagcca gtactgcacc
    25261 taccagtgca actttaataa atttgttcat ttacattccc tatgtattat atattga
//
LOCUS       DAZFKY010000056        25213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFKY010000056 DAZFKY010000000
VERSION     DAZFKY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..343)
                     /gene="acpS"
                     /locus_tag="ACHKCL_002017"
     CDS             complement(<1..343)
                     /gene="acpS"
                     /locus_tag="ACHKCL_002017"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5762986.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADE"
     gene            complement(343..1074)
                     /gene="pdxJ"
                     /locus_tag="ACHKCL_002018"
     CDS             complement(343..1074)
                     /gene="pdxJ"
                     /locus_tag="ACHKCL_002018"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5762987.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1086..1814)
                     /gene="recO"
                     /locus_tag="ACHKCL_002019"
     CDS             complement(1086..1814)
                     /gene="recO"
                     /locus_tag="ACHKCL_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5762988.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(1826..2731)
                     /gene="era"
                     /locus_tag="ACHKCL_002020"
     CDS             complement(1826..2731)
                     /gene="era"
                     /locus_tag="ACHKCL_002020"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5762989.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2728..3408)
                     /gene="rnc"
                     /locus_tag="ACHKCL_002021"
     CDS             complement(2728..3408)
                     /gene="rnc"
                     /locus_tag="ACHKCL_002021"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5762990.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3681..4655)
                     /gene="lepB"
                     /locus_tag="ACHKCL_002022"
     CDS             complement(3681..4655)
                     /gene="lepB"
                     /locus_tag="ACHKCL_002022"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5762991.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4671..6470)
                     /gene="lepA"
                     /locus_tag="ACHKCL_002023"
     CDS             complement(4671..6470)
                     /gene="lepA"
                     /locus_tag="ACHKCL_002023"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5762992.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6668..7147)
                     /gene="rseC"
                     /locus_tag="ACHKCL_002024"
     CDS             complement(6668..7147)
                     /gene="rseC"
                     /locus_tag="ACHKCL_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5762993.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7144..8100)
                     /gene="rseB"
                     /locus_tag="ACHKCL_002025"
     CDS             complement(7144..8100)
                     /gene="rseB"
                     /locus_tag="ACHKCL_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5762994.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8100..8738)
                     /gene="rseA"
                     /locus_tag="ACHKCL_002026"
     CDS             complement(8100..8738)
                     /gene="rseA"
                     /locus_tag="ACHKCL_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5762995.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8771..9346)
                     /gene="rpoE"
                     /locus_tag="ACHKCL_002027"
     CDS             complement(8771..9346)
                     /gene="rpoE"
                     /locus_tag="ACHKCL_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5762996.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9343..9498)
                     /gene="rseD"
                     /locus_tag="ACHKCL_002028"
     CDS             complement(9343..9498)
                     /gene="rseD"
                     /locus_tag="ACHKCL_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5762997.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            9754..11376
                     /gene="nadB"
                     /locus_tag="ACHKCL_002029"
     CDS             9754..11376
                     /gene="nadB"
                     /locus_tag="ACHKCL_002029"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5762998.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11361..12098)
                     /gene="trmN"
                     /locus_tag="ACHKCL_002030"
     CDS             complement(11361..12098)
                     /gene="trmN"
                     /locus_tag="ACHKCL_002030"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5762999.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12230..13564
                     /gene="srmB"
                     /locus_tag="ACHKCL_002031"
     CDS             12230..13564
                     /gene="srmB"
                     /locus_tag="ACHKCL_002031"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5763000.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(13597..14478)
                     /gene="yfiE"
                     /locus_tag="ACHKCL_002032"
     CDS             complement(13597..14478)
                     /gene="yfiE"
                     /locus_tag="ACHKCL_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5763001.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            14581..15168
                     /gene="eamB"
                     /locus_tag="ACHKCL_002033"
     CDS             14581..15168
                     /gene="eamB"
                     /locus_tag="ACHKCL_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5763002.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15224..15607)
                     /gene="grcA"
                     /locus_tag="ACHKCL_002034"
     CDS             complement(15224..15607)
                     /gene="grcA"
                     /locus_tag="ACHKCL_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5763003.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            15912..16601
                     /gene="ung"
                     /locus_tag="ACHKCL_002035"
     CDS             15912..16601
                     /gene="ung"
                     /locus_tag="ACHKCL_002035"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5763004.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(16649..17686)
                     /gene="yfiF"
                     /locus_tag="ACHKCL_002036"
     CDS             complement(16649..17686)
                     /gene="yfiF"
                     /locus_tag="ACHKCL_002036"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5763005.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            17893..18312
                     /gene="trxC"
                     /locus_tag="ACHKCL_002037"
     CDS             17893..18312
                     /gene="trxC"
                     /locus_tag="ACHKCL_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5763006.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18381..19079
                     /gene="tapT"
                     /locus_tag="ACHKCL_002038"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18381..19079
                     /gene="tapT"
                     /locus_tag="ACHKCL_002038"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5763007.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19111..21771
                     /gene="pat"
                     /locus_tag="ACHKCL_002039"
     CDS             19111..21771
                     /gene="pat"
                     /locus_tag="ACHKCL_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5763008.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            21885..23240
                     /gene="pssA"
                     /locus_tag="ACHKCL_002040"
     CDS             21885..23240
                     /gene="pssA"
                     /locus_tag="ACHKCL_002040"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5763009.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVLRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23286..23609
                     /gene="yfiM"
                     /locus_tag="ACHKCL_002041"
     CDS             23286..23609
                     /gene="yfiM"
                     /locus_tag="ACHKCL_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5763010.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(23606..24904)
                     /gene="kgtP"
                     /locus_tag="ACHKCL_002042"
     CDS             complement(23606..24904)
                     /gene="kgtP"
                     /locus_tag="ACHKCL_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5763011.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25021..>25213)
                     /locus_tag="ACHKCL_002043"
     CDS             complement(25021..>25213)
                     /locus_tag="ACHKCL_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763012.1"
                     /translation="KAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT         6060 a         6703 c         6332 g         6118 t
ORIGIN      
        1 gctcatcggc cagcgtcaca tgcatatttg caacgcccag cttttccgcc agttttaatg
       61 cctcgcccca cagtcgcagc cgtggcttac caagctcatc gttaaatact tcaaactgat
      121 taaacgccag accattgcgg atcccggtgc caaacgcttt tgctgcggct tcttttacgg
      181 caaagcgttt cgccagaaaa cgcaccggct ggtggtgcgt tttccaaatt tcccattcgt
      241 tatcgcttaa tacgcggcgt gccaggcgct caccggatcg ggcgatcact gcttctatgc
      301 gagcgatctc tacgatatcc gtgcctaaac ctaatattgc cattagccac gcgcttccag
      361 catcaggcgc ttcatttctg ccaccgcatc tttcagtccg gtcatcactg cacgaccaat
      421 aatggcatga ccgatattca gttcgtgcat ctcagggatg gcggcaatgg cttttacgtt
      481 gtgataggtc agaccgtgtc cggcgttaac tttcaaaccg agactagcgg caaaggtcgc
      541 ggctttggcg atacgcgcca gctcttgcgc ctgttcggcg tcagttttgg catcagcata
      601 gcaaccggtg tggatctcga taaacggtgc gccaacctct gccgcagctt tgatctgctc
      661 ttcatcggcg tcgataaaca gagaaacctg aatcccggca tccgccagac gtttgcaggc
      721 atcgcgcatt ttgtcacgct gccctgcaac gtccaggccg ccttcggttg ttacttcctg
      781 acgcttttct ggtaccaggc agcaaaaatg cggcttcgtc tcaaccgcga tcgccagcat
      841 ctcttcggtc accgccatct ccagattcat gcgggtatcc agcgtctgac gcaggatgcg
      901 cacatcgcgg tcagtaatgt ggcggcgatc ttcacgtaaa tgcacggtaa tgccgtccgc
      961 ccccgcctgc tcggcaataa acgcggcctg caccggatcc gggtaagcgg taccgcgcgc
     1021 gttgcgcagc gtagcgatat ggtcaatgtt gacgcctaac agtaattcag ccatgacaat
     1081 cctcatcatt cataatgtgt tttcaccgtt cgcttaggca taaattgccg gaacagttcc
     1141 ctgctcttta aaggtttacc gccaagatac ggcttaagcg ccatgcgggt aaagcgtttc
     1201 gcggcgcgca gtgtgtctgc gtcaggaaat tcccgtgcgt ttaacgcttt taactgcctt
     1261 ccggtgaacg ttttattgtc gataacgacg cttgcgataa accctttttc ttcgcgataa
     1321 cgatacgtca tggtgtcatc taccggctcg ccgctacccg cacaatgggt aaaattgacg
     1381 ccataaccca gatgcccgag cagtgccagt tcaaagcggc gcagcgcggg ttctggcgta
     1441 ccagtgaccc ctgcaagaga ctgaatgcag tgcaagtaat cgaaaaagag ttcagagaag
     1501 cgcgtctcgt attccagtac gcgggagaga agttcgttga tgtacagacc gctgtaaagc
     1561 gtgataccgc ttaatggcag cgccagcgag acagcttcag cactgcgcag cgttttgact
     1621 tcgccacgcc cgccaaaacg tagcaagaga ggggtgaaag gctgtaatgc acctttcagg
     1681 gtagagcgtt tagagcgtgc gcctttggca accagacgca cgcgacccga ttcctccgtg
     1741 aagacgtcca gcatcaggct ggtttcgctc cacgggcgac tatgcaggac aaatgcgcgc
     1801 tgccagcctt ccatcggagt tactcttaaa gatcgtcaac gtaaccgaga ctgcgcagtg
     1861 cgcgttcgtc gtcggcccaa ccggatttca cttttaccca cagctcaagg tgaacaggcg
     1921 cttcaaacat ttcctgcatg tctttacgcg cttcaatccc gatggttttg atcttggccc
     1981 ctttgttgcc aatgaccatc ttcttctgcc cttcacgctc aacgagaatc aaaccgttga
     2041 tgtcataacc accgcgttcg ttagagacga aacgttcgat ctccacggtc acggagtacg
     2101 gcagttcagc gccgaggaaa cgcatcagtt tttcgcggat gatttcagac gccataaaac
     2161 gctgtgagcg atcggtgatg taatcttccg ggaagtgatg agtcgcttca ggtagatgct
     2221 tacgcacgat tgccgcaata gtggcaacat tcagcccggt ttcggcagag attggcacga
     2281 tatcgaggaa gttcatctgg cttgccagga actgcaggtg cggcagcaga tcggctttct
     2341 cctgcacgtt gtccactttg ttcaccgcga ggattaccgg cgctttgcct tcgcgcagtt
     2401 tgttgagcac catttcgtcg tccggcgtcc agcgggtgcc ttcaacgaca aaaatcacca
     2461 gctcaacatc gccaatagag ctgctcgccg ctttgttcat caggcggtta atagcgcgtt
     2521 tttcttccat atgcaggccc ggtgtatcga cgtagatcgc ctgatacgcg ccttcagtat
     2581 ggatccccac aatgcggtga cgagttgtct gcgccttgcg ggaggtgatg gagattttct
     2641 gccccagcag tttgttcaac aatgtggatt tgccaacatt cggacgtccg acgatggcaa
     2701 taaatccgca gtaactttta tcgatgctca ttccagctcc agttttttca acgcctgttc
     2761 ggcggcagcc tgctcagcct tacgacggct tgaacctgtg ccaaccaccg gttcactcag
     2821 gccgctgacc tggcagtgga ttgtaaattc ctgatcgtgc gcttcgccac ggacctgaac
     2881 taccagataa gtcggcagcg gcagatgacg accctgcaaa tattcttgca agcgcgtttt
     2941 cggatctttt tgtttatcgc ctgggctaat ttcgtccaga cgagtttgat accagttgag
     3001 gattaatttc tcgacggttt gaatatcact gtcgaggaat acgccaccaa ttaatgcttc
     3061 gacggtgtcg gcgagaattg actcacgacg aaatccaccg cttttaagtt cacctggtcc
     3121 taaacgtaag cactcgccta actcaaattc gcgcgccagt tccgccagcg tattgccacg
     3181 gaccagcgtg gcgcgcatcc ggctcatatc gccttcatcc acacgaggga aacggtgata
     3241 aagcgcattg gcgataacgt agctcagaat agagtcgcct aaaaattcta aacgctcgtt
     3301 atgtttgctg ctggcactac gatgagttaa tgcctgctgc aacagttcct gatgattaaa
     3361 agtgtagccc agcttccgtt gaagccgatt aattacgatg gggttcatgc gataccaata
     3421 aataaatgcg tcaacaattc agcacacgaa acagacctga tatacatggc tctgctaact
     3481 gcttcgctgc agtttcagtt tagcggtata tggggaccaa cgctgtttcg tgtgccgtgg
     3541 caacctggag gtgccaacct taaacttcgg gggaatattc tatacacaac gacgggggat
     3601 gtcgttagcc acgggagatt tatctcataa ataattcacg ttgtcgccat aacggcgaca
     3661 acgtgaacga agatgagcta ttaatggatg ccgccaatgc gacttaagcg cacacctgtc
     3721 ggccattcgc cttcttgctt atcgaagctc atccagatag ccgttgcccg accgaccaga
     3781 ttcgcttcag gcacaaagcc ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcgccc
     3841 atcatgaagt attgtcccgg aggaacaatc caggttgcca gttgttgccc tggctgctgg
     3901 taatacatcc ccacctgatc ctgcgcaatc ggcactgtca gaatgcggtg cgtcacatca
     3961 cccagtgtct ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc
     4021 acttcaaaga atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa
     4081 tcgctcggtt ccacgtttga gtaggtgacc ggcagcgcgt tttcacacgc ctggccggaa
     4141 ctgcatcccg gttgaatcgt cagctctttt gagaccggat cgtaagtgac tttatcgccc
     4201 ggtaaaccca ccgcgcgctt gatataatca agctttggat cttccggata tttaaagacc
     4261 acgatatcgc cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct
     4321 ttaatgccat aagcaaactt ctctaccaga ataaaatcac cgattaacag agtcggcatc
     4381 atcgaacctg acggaatctg gaacggttca taaataaacg aacgcacaat caatacgata
     4441 gccagtaccg gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaaccttt
     4501 ttcaacgttg ctttatccag tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc
     4561 cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg
     4621 gcaatcacca gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg
     4681 tctttgccga cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc
     4741 tgcttcatgc gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata
     4801 tcgccgccat aacatttagc cagtacgttt ttacgcagct gtttcacggt ggatcgcgca
     4861 ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga
     4921 tctttcatct tctccaccaa ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc
     4981 aacgccagcg catcaacacg ttcaccgttg attaatacgt ctacacgtac catgtcggac
     5041 gcctggaagc gtttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg
     5101 cgatcgaaga agtcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga
     5161 ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg
     5221 ttgccgagat atgcctgcgg cagcagcatg tgacactctg caatcggctc gcgcagttcg
     5281 taaatgttat ttaccgcagg cagcttggat gggctgtcga cgtagataac ttcccttgac
     5341 gtggtttcaa cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca
     5401 cgttccagac gctcctggat gatctccatg tgcagcaggc caaggaagcc gcagcggaaa
     5461 ccaaagccca gcgcgctgga gctttccggc tcatagaaca gtgaggcatc gttcaggctg
     5521 agtttaccca gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg
     5581 gcgtatacct gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc
     5641 gccagcgtta aggtatcgcc gactggagcg ccgtggatat ctttaatcgc acatacgagc
     5701 cagcctactt cgccacattt cagttcagtg cggtcaacct gtttcggcgt gaagatgccc
     5761 agacggtcgg cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc
     5821 agggtgccgt ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccatgag
     5881 tcgataatta gtgcctgcaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc
     5941 accagacgtt cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg
     6001 tcagtggcgt cgatgccgac gatatcttca atttcttccg ccacgcgttc aggatcggct
     6061 gccggcaggt caatcttgtt cagtaccggc accacttcga gatccatttc catggcggtg
     6121 tagcagtttg ccagggtttg cgcttccacg ccctgcccgg cgtcgaccac cagcaatgca
     6181 ccttcacagg cagccagcga acgggaaact tcataggaga agtctacgtg gcccggggtg
     6241 tcgataaagt taagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt
     6301 tgcgctttga tggtaatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc
     6361 tccatttcac ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat
     6421 ttaccgtggt caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat
     6481 tattgtgcct taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt
     6541 ctgtattaat agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac
     6601 cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc
     6661 agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca
     6721 atgccacgct taagatgatc ggttgccatt ctgcccgggc agcaaactta cgcgagtagc
     6781 cacgcgcaat caggaacccg ccaataccgc cgaggaccgc accacataat gctgcgacgt
     6841 cagaagcaaa gagtagctga aatagcgaag cgatgaggaa taatcccacc agcggcgaca
     6901 tataaaccag taatgcggag ctaagcaggc tgccttcggc gatccccaat tccacttttt
     6961 gccccggcac taacggctca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa
     7021 gtttattcaa cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg catgaggctt
     7081 taacatcaca actgaccagc gcctgcccgt tttgccagga gacgacggta gcccactctt
     7141 tgatcattgc gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg
     7201 cagttcaccg acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg
     7261 tccggtgcgc aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa
     7321 gctgaataat ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag
     7381 cggacgtcga ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct
     7441 gaatttagct ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccag
     7501 cgtctgcata ctgctgctga tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc
     7561 cagcgtttca ccatcgcgat caagaagatc aacccgcatc ggtaatttcg attcggtgtc
     7621 catccacacg atgtagctgt agcgtgtacc atctcgggca accacgcgaa tgacttcgca
     7681 aagacgatca gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg
     7741 tttgaaatcg gtatagatga gcgatggcag agaatcaaca atgtaatcgc cattaagcgt
     7801 gaacggttca agtcccggtt caaaatagct gatttcattg ccgcgctgta ccacttcccg
     7861 gcgcgggcca tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg
     7921 ataacgcaga gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag
     7981 tgactgactg gccaggttca tctgctgtaa taacgccccg gacgcgggag tggccgaggc
     8041 gttagcagag aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat
     8101 tactgcgatt gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgggtt
     8161 tgcgcctgct caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc
     8221 aacattgcat taatgcgacg acgctgctgc tgttgaccat tatttgcggt cgcttcagaa
     8281 ggtactccca ggctaaccgg gctggcttta cccatcatcg gcagtgtatt aaataccggc
     8341 gtttcgggct gctgggacgt ttcagattgt ccattatagt gctggacgcc aacgataact
     8401 gcaagcgata cgcatgcggc tacgcccatt tgggtaagct gtgccgccca cggacgtact
     8461 ttctgccaga atggcatttt ctgccattca tgcggcgcag gctgggcttc cgggatcaat
     8521 gtcgccggtt gacgtactgg ctcttcttca atggcggcca tcacgcgtga agagatatcg
     8581 aaatggagca cctcgggagt atcaccccgc attgagtcac ggattaagtg atagctttcc
     8641 caggttttct gcatttctgg gttatgagcc agttcgttaa gcagctcact atccagcgtt
     8701 tcgccatcca ttaaagcgga aagttgttct ttctgcatgc ctaataccct tatccagtat
     8761 cccgctatcg tcaacgcctg ataagcggtt gaactttgtt atcaatagct tccctcgctc
     8821 ggaagatacg tgaacgcacc gtacctaccg gacaatccat gatagcggct atctcttcat
     8881 agctcaggcc atccagctcc cgcaaggtta ttgccatgcg taaatcttcc gggagggact
     8941 caatagttcg gaaaactatc tgtctcagtt cttctgacaa cattaagttc tcagggttcg
     9001 aaatttcttt caacgcgccg ccactttcga agttttcagc ttcaatggca tccacatcac
     9061 tggaaggtgg acgacgcccc tgagcaacca ggtaattttt cgctgtattt acagcaatcc
     9121 gatacagcca tgtataaaaa gcgctatctc cccggaacga atccagcgca cgataggctt
     9181 taataaaagc ttcttgtacc acatcgggaa catcacccga cggcacatag cgggaaacca
     9241 gactcgccac tttatgctga tagcgcacta ccagtaagtt aaaggctttc tgatctccct
     9301 tctggacccg ttcaaccagg acctggtccg ttaactgctc gctcatccga ggtaaagtct
     9361 ccccaaacca aatttccacg cgctatcgaa acgccactcc attagctgca atttgagcaa
     9421 gcaaagggtt agagtgtctc gtttttgtaa agttccgtaa cgcatctgtt tttgtttgtc
     9481 atgctgtaga cggatcatta tctatcatta taagtctaca gaatctgaac atcgcattat
     9541 ctgtgtagaa atgcccattt aactgcctga agagtaaccc aacggccttt ttatttcacc
     9601 acctaatcct ccaccagcca gtaacttctc tttttctcgc cgccctgcgt cagcgtgttt
     9661 agtcactgta acaaatatta aaatagcagg tgtttattcg cacaacatga tgctatgctg
     9721 accaaaccgt gtttagtaaa ttaaacaaag aaaatgaata ctctccttga acattcatgt
     9781 gacgtgttga ttatcggtag cggcgcagcc gggctttcac tggcgctacg cctggctgac
     9841 cagcatcagg tcatcgttct aagtaaaggc ccggtaacgg aaggttcaac attttatgcc
     9901 cagggcggta ttgccgccgt gtttgatgaa actgacagca ttgactcgca tgtggaagac
     9961 acattgattg ccggggctgg tatttgcgat cgccatgcag ttgaatttgt cgccagcaat
    10021 gcacgatcct gtgtgcaatg gctaatcgac cagggggtgt tatttgatac ccacattcag
    10081 ccgaatggcg aagaaagcta ccatctgacc cgtgaaggtg gacatagtca ccgtcgtatt
    10141 cttcatgccg ccgacgcaac tggtagagaa gtagaaacca cgctggtgag caaggcgctg
    10201 aaccatccga atattcgcgt gctggagcgc agcaacgcgg ttgatctgat tatttctgac
    10261 aaaattggcc tgccgggcac gcgacgggtt gttggcgcgt gggtatggaa ccgaaataaa
    10321 gaaaaggtgg aaacctgcca cgcaaaagcg gtggtgctgg caaccggcgg tgcgtcgaag
    10381 gtttatcagt acaccaccaa tccggatatt tcttctggcg atggcattgc tatggcgtgg
    10441 cgcgcaggct gccgggttgc caatctcgaa tttaatcagt tccaccctac cgcgctgtat
    10501 cacccacagg cacgcaattt cctgttaacg gaagcactgc gcggcgaagg cgcttatctc
    10561 aagcgcccgg atggcacgcg ttttatgccc gattttgatg tgcgcggcga actggccccg
    10621 cgcgatattg tcgcccgcgc cattgaccat gaaatgaaac gcctcggcgc agattgtatg
    10681 ttccttgata tcagccataa gcccgccgat tttattcgcc agcattttcc gatgatttat
    10741 gaaaagctgc tcgggctggg gattgatctc acacaagaac cggtaccgat tgtgcctgct
    10801 gcacattata cctgcggtgg tgtaatggtt gatgatcatg ggcgtacgga cgtcgagggc
    10861 ttgtatgcca ttggcgaggt gagttatacc ggcttacacg gcgctaaccg catggcctcg
    10921 aattcattgc tggagtgtct ggtctatggc tggtcggcgg cggaagatat caccagacgt
    10981 atgccttatg cccacgacat cagtacgtta ccgccgtggg atgaaagccg cgttgagaac
    11041 cctgacgaac gggtaataat tcagcataac tggcacgagc tacgtctgtt tatgtgggat
    11101 tacgttggca ttgtgcgcac aacgaagcgc ctggaacgcg ccctgcggcg gataaccatg
    11161 ctccaacaag aaatagacga atattacgcc catttccgcg tctcaaataa tttgctggag
    11221 ctgcgtaatc tggtacaggt tgccgagttg attgttcgct gtgcaatgat gcgtaaagag
    11281 agtcgggggt tgcatttcac gctggattat ccggaactgc tcacccattc cggtccgtcg
    11341 atcctttctc ccggcaatca ttacataaac agataaaaag cctgggttag cgccgtatac
    11401 gcttcggaat agttctggtc tggcccacga atgactaagc gatcgctaaa gcattctccc
    11461 gcctgcgggg agaatgccag cagcacccga tgcggcagtc gcgcttcgtt ttccgccaca
    11521 tccgtccgca aacgtaaatg ccagcccata cttaatgcca gctccgtaaa actattacca
    11581 atctgctctg gcagcaccac gcagaaaaat ccctcttcgg taatgcactc cgccgcgcaa
    11641 gtaagtaaag tctggtgatc aagtgaggtg gtgtagcgcg cctgttcccg ctgcggcgtc
    11701 gcgcactcca ctccttgctc ataatacggc gggttgctga tgattaaatc aaagcgcgcg
    11761 gtctgctgtg ttacccactg ctgaatatcc gccgtatgga cgttaattct ctctaaccac
    11821 ggggactggg cgacattttc ctgcgcctga gtggcagctt cactttccag ctcaacggca
    11881 tcaattatca cgctgtcact ggttcgctgc gccagcatca gtgccagcaa gccactaccc
    11941 gcaccgatat caaggcaacg ttttacccca gccaccggtg cccatgcgcc cagcaatatg
    12001 ccatccgttc ccactttcat cgcacagcga tcgtgagcaa caaaaaactg tttaaaagta
    12061 aatccattac gacgaagcac ggatgtagac tgtgacatga aaataaaacc ttgcaggaaa
    12121 aacggcgata gcaccgggtg agaacaatac ccgagaagcg atatccatac aaacagatga
    12181 agattgcagc cgtaacgtct ataatcagcg ccccacacag aggtagaaca tgactgtaac
    12241 gactttttcc gaacttgaac tcgacgaaag cctgctggaa gccctccagg ataaaggttt
    12301 cactcgcccg accgccattc aggctgccgc cattccgcct gcgctcgatg gccgtgatgt
    12361 actcggttct gcgccgacag gcaccggtaa aacggcggcg tatctgctgc cagcgttgca
    12421 gcacctgctc gatttcccgc gtaagaaatc cggtccgccg cgtattttga tcctcacccc
    12481 aactcgcgag ctggcgatgc aggtggccga tcatgcccgc gaactggcga aacatacgca
    12541 tctggatatc gccaccatca ccggcggcgt agcctatatg aaccacgcgg aagtgttcag
    12601 cgaaaatcag gacatcgtgg tcgccacgac cggacgtctg ctgcaataca taaaagaaga
    12661 gaacttcgat tgccgcgcgg ttgaaacgct gatcctcgac gaagcagacc gtatgctgga
    12721 tatgggcttc gctcaggata tcgaacatat tgctggcgaa acgcgctggc gtaaacagac
    12781 cctgctcttt tcggcaacgc tggaaggcga tgcgattcag gactttgccg agcgtctgct
    12841 ggaagatccg gtggaggttt ctgccaatcc gtccacccgt gagcgcaaaa aaattcatca
    12901 gtggtattac cgcgccgatg acctggaaca taaaaccgca ttgctggtgc atctgttaaa
    12961 acagccggaa gcgacccgct caattgtgtt tgtgcgtaag cgtgagcgtg tgcatgagct
    13021 ggcaaactgg ctgcgcgaag cgggcatcaa caactgctat ctcgaaggtg agatggtaca
    13081 gggcaagcgt aacgaagcga tcaaacgttt gaccgaaggt cgcgtaaacg tactggttgc
    13141 aaccgatgtt gccgcgcgcg gtatcgacat tcctgacgtc agccacgtct ttaacttcga
    13201 tatgccgcgc agtggcgata cttatttgca ccgtatcgga cgtaccgcgc gcgccggtcg
    13261 taaaggcacc gcaatttcgc tggtagaagc ccatgaccat ctgctgctgg gcaaagttgg
    13321 tcgctatatt gaagagccaa ttaaagctcg cgtaattgat gagttacgcc cgaaaacgcg
    13381 tgcaccaagc gagaagcaga ccggcaagcc atcgaagaaa gtactggcaa aacgcgccga
    13441 gaagaaaaaa gcaaaagaga aagagaagcc gcgggtgaaa aaacgccatc gcgataccaa
    13501 aaatattggt aagcgccgta aaccaagcgg aacgggcgtg ccaccgcaaa cgacagaaga
    13561 gtaatctcaa tgccgggttt aagcccggca ttatttttat cccggcaaaa aacaatcttc
    13621 aacacactga ataaaagtgt gcatcgccgg gctaaccgct tttccggcat ggtgagcgca
    13681 cattgccgta atggtctgcg agtgttcgcc aaaaggcaat tcaattaatt ctccgctctc
    13741 aagctctttt tccaccgcaa agcggggcaa gtaactgaca ccgatattcg ccgcgacaca
    13801 acgtttgata ctttcaatgc ttaacagctc aatggtgttt tccaccgtga tccgccgctg
    13861 gcgcagcgtg ctctcaaata tctgccggaa tacacattgc ggttcgttga taataaagct
    13921 acaggcgtta tgtcttcccg gctcagtaaa atcgacatct gcaatttgcg gtgaagctac
    13981 cagcaccagc gattgctcac ccaattctcg tcggttcagg gcatcatcat tccctacgcg
    14041 ataaaaaacc ccgacatcgg cctcatcatt caatagcgca tcacggatca cgtaacagtt
    14101 cagagactgc aacgataaac gcacttttgg ggcgcgttgc cgaaaacgct gcaacacctg
    14161 cggcattcgg taagagagca acgtttcgcc cgaaacaacg cgtagttccc cgtccggatc
    14221 cgactccttt ttggcagcct cacgaagggt atccatcact ctggtgagtt cataaatgtg
    14281 cggcagcagc tttttacctt cgcgagtgag gcacatccgt cggccaattt tctcaaataa
    14341 ctgtaccgag aactcctgct caagctgctg aatatgaaaa gtcaccgtcg attgtgtaca
    14401 gcacaatttt tgcgaagctc gcaaaaagga accctcttcc accacggttt taagcgtaat
    14461 aaaacggcgc agatccataa ccccaaacct atcgaaaata tcgaatttag aatgtaaaaa
    14521 cattcatttt tttaaatatt ctgtgtcggg tactgtctac caaaacagag gagataacaa
    14581 gtgacaccga cccttttaag tgctttttgg acttacaccc tgattaccgc catgacgcca
    14641 ggaccgaaca atattctcgc ccttagctca gccacaacgc acggatttca tcagagtacc
    14701 cgcgtgctgg cagggatgag tctgggattt ttaatcgtca tgttgctgtg tgcgggcatt
    14761 tcgttttcac tggctgttat cgacccggca gctgtgcatc tgttgagttg ggcgggggcg
    14821 gcatatattg tctggctggc gtggaaaatc gctaccagtc caacaaagga agacgggctt
    14881 cagacaaaac caatcagttt ttgggccagc tttgctttgc agtttgtgaa cgtcaaaatc
    14941 attttgtacg gtgttacggc actgtcgaca tttgttctgc cgcaaacaca ggcgttaagc
    15001 tggatagttg gcgtcagcgt tttgctggca atgattggga cgtttggtaa tgtgtgctgg
    15061 gcgctggcgg ggcatctgtt tcagcgattg tttcgccagt atggtcgcca gttaaatatc
    15121 gtgctggcgc tgctgctgat ctattgcgcg gtacgcattt tctattaatg aaaaaaagcg
    15181 gaagaggtcg ccctcttccg cttagtaaca tgctacttaa gccttacagg ctttcagtaa
    15241 aggtacgagc gataacgtcg cgctgctgtt ccggagtcag agagttaaag cgaactgcat
    15301 aaccggatac acggatggtc agctgcggat atttttccgg atgcttaact gcatcttcca
    15361 gagtttcacg acgcagaacg ttaacgttca ggtgctgacc accttcaacg cgaacttctg
    15421 gtttcacttc tactggaact tcacggtact caatgtcacc cagtttgctt actgcaacca
    15481 cttcgtcttc tgcataacct gctttcgcaa cgatgcaacg cgcttcgcct ttttcgctgt
    15541 ccagcagcca gaaagagttc agcagatcgt cgttagcggc tttagtaatc tggatacctg
    15601 taatcatgtg atgcctcccc ggcaaaatta tttgatttgt tcagcctgtc gcggccaatt
    15661 ggtaaaacca ttgttgcttg agtgtatata tactcctcaa acacccttga atctttgatt
    15721 taaatcaata aaaaccacac atcaagtgtg gtcgcaaata gattttattg ttttacatca
    15781 acttatgcgg gtgtgaaatt ttaccaattt acattttttt gcactcgttt aagtctaaaa
    15841 aatgagcatg attttgttct gtagaaagaa gcagttaagc taggcggatt gaagattcgc
    15901 aggagagcga gatggctaac gaattaacct ggcatgacgt gctggctgaa gagaagcagc
    15961 aaccctattt tcttaatacc cttcagaccg tcgccagcga gcggcagtcc ggcgtcacta
    16021 tctatccacc acaaaaagat gtctttaacg cgttccgctt taccgagttg ggtgacgtta
    16081 aagtggtgat tctcggccag gatccttatc acggaccggg acaggcgcat gggctggcat
    16141 tttccgttcg ccccggaatc gccactcctc cgtcactgct gaatatgtat aaagagctgg
    16201 aaaatactat tccgggcttc acccgcccta atcatggtta tcttgaaagc tgggcgcgtc
    16261 agggcgttct gctactcaat actgtgttga cggtacgcgc aggtcaggcg cattcccacg
    16321 ccagcctcgg ctgggaaacc ttcaccgata aagtgatcag cctgattaac cagcatcgcg
    16381 aaggcgtggt gtttttgttg tggggatcgc atgcgcaaaa gaaaggggcg attatagata
    16441 agcaacgcca tcatgtactg aaagcaccgc atccgtcgcc gctttcggct catcgtggat
    16501 tctttggctg caaccatttt gtgctggcaa atcagtggct ggaacaacat ggcgagacgc
    16561 cgattgactg gatgccagta ttaccggcag agagtgagta aatttgcggg aaaatgccgg
    16621 atggcagagt tgccacccgg ctgatttatc aggctttatt ctgacgccac cattcgccaa
    16681 gaagaacgcc ggttgcgaca gagatattca gcccggcaac gttgccagta ccatcaatct
    16741 tcacgcgcag atcgttcgga tcgcgtgcgg catccggtaa cccttcatat tcctgaccca
    16801 gcaccagtac cattttcgct ggcagactgg ttttgaacag cggtttaccc tgctcgctgg
    16861 aagttgtcac tacggtgtaa cccgcctgac ggaaatcatc cagcacgtta acaatgttgt
    16921 cgccagtaat cggctgaacg tgctctgcgc cgccttctgc ggtacggata gccgcccccg
    16981 actccagcag tgccgcatcc tgcaccacaa cacctttcac gccaaagtgc gcgcagctgc
    17041 gcatcatgcc acccaggtta tgcgggttag attcgttttc cagtgccaga acacaatcct
    17101 gcgcgcctgc ctggcttacc cactgctgca cggttgtgcc attacgcttt ttgatcagga
    17161 agcaaacgcc accgtgatgt tccgtgcctg acgcttttgt caattccgct tcatctacca
    17221 catggtacgc tttgcggttt gctgccatcc agcgcaaggc ttctttaaaa cgcggcgtta
    17281 cactctggat aaaccaggcg cgaacaatcg cttccggacg gctctggaac agtgcctgac
    17341 aggcgttttc gccgtagacg cgggtttctt ccgcacgctg acgacgcaac acttccggat
    17401 caataaaact tttaccactg atgccaccgt gatcggcctt ttccggcgtc tcatcacccg
    17461 gcgcgcggga aaccgtgcgc cacggcgaat cttcccactt gcggtcacgg ggctgactct
    17521 gtttgtcatc gcgggcgggg cgacggccac cgtcagcacg agattttcct ggacgcccgc
    17581 cccctttccc ggtacgcggg ttgtgggtac gtttatcaga atcatcatca ctgcggacat
    17641 acatcacttt gaccttgccg cttttacctt tcatttcatc gttcatgctt ttctccacca
    17701 gcgctgcgcg aagcgcgcag attacccgaa gtccgcgcgg ttcgccatga tttcgtacca
    17761 aagcctgcga ctatcatacc tattgaataa aacagattgt tgtctggaac aatgtccccg
    17821 ataatatgta acatattaga aacataccgg cgttgttgcc gataagtctc cttactcatc
    17881 ccgaggttag ttatgaatac cgtttgtacc cattgtcagg ccatcaatcg cattcccgac
    17941 gatcggatcg aagatgcggc aaaatgcgga cgctgcggtc acgacttgtt tgacggagag
    18001 gtgattaatg cgaccggtga aacgctcgac aaattgctga aagatgatct accagtggtg
    18061 atcgacttct gggcaccgtg gtgcggcccc tgccgtaatt tcgcaccaat ttttgaagat
    18121 gtcgcgcaag agcgtagcgg taaagtgcgc tttgtgaaag tgaataccga agctgaacgt
    18181 gaattgagca gtcgctttgg aattcgtagt ataccgacga tcatgatttt caaaaacggt
    18241 caggttgtcg acatgcttaa tggcgcagta ccgaaagcgc cgttcgatag ctggctgaac
    18301 gaatctcttt aatcttaccg gggcgcatct tgtgccccgt tttctcctct gcgacaatgg
    18361 cgttttttcg acgctctctt atgaccgaaa acgctgttct ccagttacgc gccgagcgta
    18421 ttgcgcgcgc aacacgtcct tttcttgccc gcggtaatcg cgttcgtcgc tgccaacgct
    18481 gtcttttgcc agagaaatta tgtctctgtt cgacaattac cccagcacaa gcaaaaagtc
    18541 gcttctgttt gctgatgttc gacaccgagc caatgaagcc cagtaatacc gggcgtctga
    18601 ttgctgatat tttgcctgat accgttgcgt ttcaatggtc gcgtaccgaa ccctcgcagg
    18661 atttgctgga tctggtacaa aacccggatt atcagccaat ggtggtcttt cccgcctcgt
    18721 atgctgatga gcaacgggaa gtgatcttca caccacctgc cggtaagcca ccgctgttta
    18781 tcatgctcga tggtacctgg ccggaagctc gcaagatgtt tcgtaaaagt ccgtatctgg
    18841 ataatcttcc cgtcatttcc gtcgatcttt cccggctttc tgcctatcgc ctgcgtgaag
    18901 cccaggctga aggccaatat tgtactgccg aggtagccat cgcactgtta gatatggccg
    18961 gcgataccgg ggcggcagca gggttaggcg agcacttcac ccgctttaaa acgcgctatc
    19021 tggcaggaaa aacgcaacat ctgggtagca tcacagcaga acagttagaa agcgtttaaa
    19081 atcattcggt cacttctgcg ggagaccggt atgagtcagc gaggactgga agcactactg
    19141 cgaccaaaat cgatagcggt aattggcgcg tcgatgaaac ccaatcgcgc aggttacctg
    19201 atgatgcgta acctgctggc gggaggcttt aacggaccgg tactcccggt gacgccagcc
    19261 tggaaagcgg tgttgggtgt gttggcctgg ccggatattg ccagcttgcc ctttacaccc
    19321 gaccttgcgg ttttatgtac caatgccagc cgtaatcttg ctcttctgga agagctcggc
    19381 gagaaaggct gtaaaacctg cattattctt tccgccccgg catcgcaaca cgaagatctc
    19441 cgcgcctgcg ccctgcgcca taacatgcgc ctgcttggac caaacagtct gggtttactg
    19501 gctccctggc aaggtctgaa tgccagcttt tcgcctgtgc cgattaaacg cggcaagctg
    19561 gcgtttattt cacagtcggc tgccgtctcc aacaccatcc tcgactgggc gcaacagcgt
    19621 gagatgggct tttcctactt tattgcgctc ggcgacagcc tagatatcga cgttgatgaa
    19681 ttgcttgact atctggcacg cgacagtaaa accagcgcca tcctgctcta tctcgaacag
    19741 ttaagcgacg cgcgacgctt tgtttcggcg gcccgtagtg cctcgcgtaa taaaccgatt
    19801 ctggtgatta aaagcggacg tagcccggca gcacagcgac tgctcaacac gacggcagga
    19861 atggatccgg cgtgggatgc ggcgattcag cgtgccggtt tgttgcgggt gcaggatacc
    19921 cacgagctgt tttcggcggt ggaaaccctc agccatatgc gcccgctgcg tggcgaccgg
    19981 ttgatgatta tcagcaacgg tgctgcgcct gccgcgctgg cgctggatgc cttatggtca
    20041 cgcaatggca agctggcaac gctaagcgaa gaaacctgcc agaaactgcg cgatgcactg
    20101 ccaggacatg tggcaatctc taacccgctc gatctgcgcg atgacgccag cagcgagcat
    20161 tatgtcaaaa cgctggacat tctgctccac agccaggatt ttgatgcgct gatggttatt
    20221 cattcgccca gcgccgctgc tcccgcaaca gaaatcgcgc aagtattaat tgaagcggta
    20281 aagcatcatc cccgcagcaa gtatgtttct ctgctgacga actggtgcgg cgagcactcc
    20341 tcgcaagagg cacgacgttt attcagcgaa gccgggctgc cgacctaccg taccccggaa
    20401 ggaaccatca ctgcttttat gcatatggtg gagtaccggc gtaatcagaa gcaactacgc
    20461 gaaacgccgg cgttgcccag caatctgact tccaataccg caaaagcgca tcttctgttg
    20521 caacaggcga ttgccgaagg ggctacgtcg ctcgataccc atgaagttca gcccatcctg
    20581 caatcgtatg ggatgaacac gctccctacc tggattgcca gcgatagcac cgaagcggtg
    20641 catattgccg aacagattgg ttatccggtg gcgctgaaat tgcgttcgcc ggatattcca
    20701 cataaatcgg aagttcaggg cgtcatgctg tacctgcgta cagccaatga agtccagcaa
    20761 gcggcaaacg ctattttcga tcgcgtaaaa atgacctggc cgcaggcgcg ggtccacggc
    20821 ctgttggtgc aaagtatggc taaccgtgct ggcgctcagg agttgcgggt tgtggttgag
    20881 cacgatccgg tttttgggcc gttgatcatg ctgggtgaag gcggtgtgga gtggcgtcct
    20941 gaagatcaag ccgtcgtcgc actgccgccg ctgaatatga acctggcccg ctatctggtt
    21001 attcagggga tcaaaagtaa aaagattcgt gcgcgcagtg cgctacgccc attggatgtt
    21061 gcaggcttga gccagcttct ggtgcaggtt tccaacttga ttgtcgattg cccggaaatt
    21121 cagcgtctgg atattcatcc tttgctggct tctggcagtg aatttaccgc gctggatgtc
    21181 actctggata tcgcgccgtt tgaaggcgat aacgagagtc ggctggcagt gcgcccttat
    21241 ccgcatcagc tggaagaatg ggtagaattg aaaaacggtg aacgctgctt gttccgcccg
    21301 attttgccag aagatgagcc acaacttcag caattcattt cgcgggtcac caaagaagat
    21361 ctttattacc gctactttag cgagatcaac gaatttaccc atgaagattt agccaacatg
    21421 acgcagatcg actacgatcg ggaaatggcg tttgtagcgg tacgacgaat tgatcaaacg
    21481 gaagagatcc tcggcgtcac gcgtgcgatc tccgaccctg ataacatcga tgccgaattt
    21541 gccgtgctgg ttcgctcgga tctcaaaggg ttaggcttgg gtcgacgctt aatggaaaag
    21601 ttgattacct atacgcgaga tcacggacta caacgtctga atggtattac gatgccaaac
    21661 aatcgtggca tggtggcgct ggcccgcaag ctcgggttta acgttgatat ccagctcgaa
    21721 gaggggatcg ttgggcttac gctaaatctt gcccaacgcg aggaatcatg agtaaggtac
    21781 tggaaatgtt gaccacttaa tcgggactgg tgttattatt gcacgcttat gtcgtctgca
    21841 ttgcacagag gacccttcaa tgaacagaga agaaatgcac tgtgatgttg tcaaaattta
    21901 agcgtaataa acatcaacaa caccttgccc aactacccaa gatttctcaa tcagttgatg
    21961 atgtcgattt cttttacgct cccgccgact tccgggagac gctgctggaa aaaatagcca
    22021 gcgcgaagca gcgcatttgc attgtcgccc tgtatctcga acaggatgac ggtggcaaag
    22081 gcattctgaa cgcgttgtat gaagctaaaa ggcagcgtcc ggaactggat gtgcgggtgc
    22141 tggtcgactg gcatcgtgca caacgtggac gcattggcgc tgcggcatct aacactaacg
    22201 ctgactggta ctgtcgcatg gcgcaggaaa atccgggcgt agatgttccg gtttatggcg
    22261 ttccaatcaa tacccgtgaa gcccttggtg ttctgcactt taaaggcttt atcatcgacg
    22321 atagcgtact ttatagcggt gccagcctga acgatgttta cctgcatcag cacgataaat
    22381 atcgctacga ccgttatcat cttatccgta accgtaagat gtcagacatt atgtttgaat
    22441 gggttacaca gaatattatg aatggccgcg gcgttaatcg tctggatgat gttaatcggc
    22501 caaaaagccc ggaaatcaag aacgatattc gtctgttccg ccaggagctg cgtgatgccg
    22561 cttatcattt ccagggcgat gccgacaacg atcagctttc cgtaacgccg ctagtggggc
    22621 tggggaaatc gagtctgttg aacaagacca ttttccatct tatgccttgt gccgagcaga
    22681 aactaaccat ctgtacgcca tacttcaacc tgccagcaat ccttgtgcgc aatattatcc
    22741 agttgctgcg cgaagggaaa aaggtcgaaa ttattgttgg tgataaaacc gcgaacgact
    22801 tctacattcc ggaagatgaa cctttcaaga taattggcgc attgccttat ctctatgaga
    22861 tcaatctgcg tcgtttcctg agccgtttgc agtattacgt caatactgac cagctagtgc
    22921 ttcggttatg gaaagatgac gacaacacct atcacctaaa agggatgtgg gttgatgata
    22981 agtggatgtt gatcaccggt aataacctga acccgcgcgc ctggcgtctg gatctggaaa
    23041 acgccatttt gatccacgat ccacaactgg agcttgcgcc gcagcgagag aaagaactgg
    23101 agctgatccg cgagcatacc accatcgtta agcactatcg cgatctgcaa agtattgccg
    23161 attatccggt gaaggttcgt aaactcatcc gccgtttgcg ccgtatccgc atcgaccgat
    23221 taattagccg catcctgtaa tcacaacccc gtcctgtacg gggtttgttt tttggaggcc
    23281 acgttttgcg tattcttttt gtctgttcgc tattgttact ttctggatgc agccatatgg
    23341 ctaacgatag ctggagcggg caggataaag ctcaacactt tatcgcctcg gcgatgcttt
    23401 ccgccgccgg aaatgaatat tcacagcatc aggggatgag ccgggatcgc agtgccatgt
    23461 ttggtttgat gttctctgtc agtttggggg cgtcaaaaga gttttgggat agccgccccg
    23521 aagggagcgg ctggagctgg aaggatttgg cctgggatgt cgccggtgca agcaccggct
    23581 ataccgtctg gcaactgacc cgtcactaaa gacgcatccc cttccctttg cgatgtagca
    23641 tcaaagaaac cagaaacgcc accacggcca tcaaggtcac ataccagaag aaggctgttt
    23701 ccattcctat tgatttcagc gacaacgcta cgtactccgc cgaaccacca aatatagcat
    23761 tagcgaccgc atatgacaga ccaacgccta atgcgcgaac ctgtgccggg aacatctcag
    23821 ccttcagtat tccactgatt gatgtataaa aactcactat cagcagggca cacatcacca
    23881 gaccaaaagc ggcataaggc gaggaaacgt tttgcaatgc tgagagaata ggaacggtaa
    23941 aaatggctgc cagcgaaccg aaacataaca ttgaggtacg gcgaccaatc ttatccgaca
    24001 gcgcgccaat gagtggttga ataagcatga atacaaacaa tgcggcagtc ataatgccac
    24061 tcgccacgtt ggcatgcatt cccgcagtat ttaccagata cttctgcata taagtggtga
    24121 aggtatagaa acaaagggag cccgcagcgg taaaaccgag aaccatgatg aatgcacggc
    24181 gattgcgcca taatcctttc agagatccag cttcttttaa agcgcgcgtt tcttgttgcg
    24241 aagtttcatc taactgacga cgtaaccaca acgccacaac agctaacaca gctcctaacg
    24301 cgaaaggaat acgccatccc cactctctga gtgcagagtc ttccatggtg tgttgtaaaa
    24361 ccacgacaac cagtagggct agcagttgtc cgccgatcaa cgtcacatac tgaaatgatg
    24421 cgtaaaaacc tttgcgccct tcaacggcaa cttcactcat ataggtggcg ctggtgccat
    24481 attctccgcc aacggataat ccctgaaata aacgcgcgag aagcaataat gccggagccc
    24541 acgtaccgat tgtttcataa ccaggcaaac aggcgattac cagtgagccg aaacacatca
    24601 tacacaccga tagcagcatc gattttttgc gaccatgttt atcggctata cggccaaata
    24661 gccaaccgcc tattgggcgc atcaggaatc ccgcagcaaa aacacctgct gtttgtagta
    24721 gttgagtcgt cgtgttcccg gaagggaaga agatgtgggc aaagtagagt gaacagaacg
    24781 agtagacata gaaatcgaac cactcgacca gattacctga agaggccccc acaatcgccc
    24841 aaatgcggcg acgagtatca ctacttgtca gtttgctgtc tgccgttaca gtactttcag
    24901 ccatgccatt atgtctcctg ccgtaatccg atgcttttgt cggtcgcttt tgtttatttc
    24961 ttttggcaaa gaaaatgtta tacatttgtt acgtatcatt atgcaacctt aaccatgaat
    25021 ttagttagca ggaaaatggt tattgaggag cttaaggata aatttctggt aaggaggaca
    25081 cgtatggaag taggcaagtt ggggaaaccg tatccgttgc tgaatctggc atatgtggga
    25141 gtataagacg cgcagcgtcg catcaggcat ttttttcacc aacgcaaaaa ggccatccgt
    25201 caggatggcc ttt
//
LOCUS       DAZFKY010000057        24766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFKY010000057 DAZFKY010000000
VERSION     DAZFKY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..734
                     /gene="yiaT"
                     /locus_tag="ACHKCL_002044"
     CDS             <1..734
                     /gene="yiaT"
                     /locus_tag="ACHKCL_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5763013.1"
                     /translation="NRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENTKAI
                     PLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDKRDS
                     TAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYY
                     DENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELP
                     EEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(864..2834)
                     /locus_tag="ACHKCL_002045"
     CDS             complement(864..2834)
                     /locus_tag="ACHKCL_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO5763014.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2845..4245)
                     /locus_tag="ACHKCL_002046"
     CDS             complement(2845..4245)
                     /locus_tag="ACHKCL_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763015.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4471..5286
                     /locus_tag="ACHKCL_002047"
     CDS             4471..5286
                     /locus_tag="ACHKCL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5763016.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5288..5428
                     /locus_tag="ACHKCL_002048"
                     /pseudo
     CDS             5288..5428
                     /locus_tag="ACHKCL_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            5530..6003
                     /gene="ysaA"
                     /locus_tag="ACHKCL_002049"
     CDS             5530..6003
                     /gene="ysaA"
                     /locus_tag="ACHKCL_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5763017.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6155..7408)
                     /gene="avtA"
                     /locus_tag="ACHKCL_002050"
     CDS             complement(6155..7408)
                     /gene="avtA"
                     /locus_tag="ACHKCL_002050"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5763018.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7586..9616)
                     /gene="malS"
                     /locus_tag="ACHKCL_002051"
     CDS             complement(7586..9616)
                     /gene="malS"
                     /locus_tag="ACHKCL_002051"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5763019.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            9936..10760
                     /gene="bax"
                     /locus_tag="ACHKCL_002052"
     CDS             9936..10760
                     /gene="bax"
                     /locus_tag="ACHKCL_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5763020.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10868..12046)
                     /gene="xylR"
                     /locus_tag="ACHKCL_002053"
     CDS             complement(10868..12046)
                     /gene="xylR"
                     /locus_tag="ACHKCL_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5763021.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12124..13305)
                     /gene="xylH"
                     /locus_tag="ACHKCL_002054"
     CDS             complement(12124..13305)
                     /gene="xylH"
                     /locus_tag="ACHKCL_002054"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5763022.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13283..14824)
                     /gene="xylG"
                     /locus_tag="ACHKCL_002055"
     CDS             complement(13283..14824)
                     /gene="xylG"
                     /locus_tag="ACHKCL_002055"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5763023.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14902..15894)
                     /gene="xylF"
                     /locus_tag="ACHKCL_002056"
     CDS             complement(14902..15894)
                     /gene="xylF"
                     /locus_tag="ACHKCL_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5763024.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16260..17582
                     /gene="xylA"
                     /locus_tag="ACHKCL_002057"
     CDS             16260..17582
                     /gene="xylA"
                     /locus_tag="ACHKCL_002057"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5763025.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17654..19108
                     /gene="xylB"
                     /locus_tag="ACHKCL_002058"
     CDS             17654..19108
                     /gene="xylB"
                     /locus_tag="ACHKCL_002058"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5763026.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19277..19618
                     /gene="yiaB"
                     /locus_tag="ACHKCL_002059"
     CDS             19277..19618
                     /gene="yiaB"
                     /locus_tag="ACHKCL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5763027.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19664..20101
                     /gene="yiaA"
                     /locus_tag="ACHKCL_002060"
     CDS             19664..20101
                     /gene="yiaA"
                     /locus_tag="ACHKCL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5763028.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20143..21138)
                     /gene="wecH"
                     /locus_tag="ACHKCL_002061"
     CDS             complement(20143..21138)
                     /gene="wecH"
                     /locus_tag="ACHKCL_002061"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5763029.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21313..21612
                     /gene="ysaB"
                     /locus_tag="ACHKCL_002062"
     CDS             21313..21612
                     /gene="ysaB"
                     /locus_tag="ACHKCL_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5763030.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21707..22618
                     /gene="glyQ"
                     /locus_tag="ACHKCL_002063"
     CDS             21707..22618
                     /gene="glyQ"
                     /locus_tag="ACHKCL_002063"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5763031.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22628..24697
                     /gene="glyS"
                     /locus_tag="ACHKCL_002064"
     CDS             22628..24697
                     /gene="glyS"
                     /locus_tag="ACHKCL_002064"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5763032.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6110 a         6107 c         6408 g         6141 t
ORIGIN      
        1 ttaaccgcaa tattgtggcg ttatttgcgt tgccttttat ggcaagcgca actgcttctg
       61 aattatccat tggtgctggt gcggcttata atgaatcgcc ttatcgcggt tataatgaaa
      121 atacaaaggc aattccgctg attagttatg aaggtgattc tttttatgtt cgtcagacca
      181 cgttaggttt tattctgtcg caaagtgaaa aaaatgaact tagcctgacc gcatcctgga
      241 tgccgctgga atttgaccct gccgataatg acgattatgc catgcaacag cttgataagc
      301 gtgatagtac ggctatggcg ggggttgcct ggtatcacca cgagcgttgg ggaaccgtga
      361 aagcctctgc agctgcggac gttctggata acagcaacgg ctgggtgggg gagctatcgg
      421 tattccacaa aatgcagata ggtcgtctgt cgctgacacc tgcgctgggc gttctctatt
      481 atgacgagaa tttcagtgac tattactatg gcatttcaga gagtgagtcc cgtcgtagcg
      541 gtctggcaag ttattccgcg caggatgcct gggtgcccta tgtcagcctg acggcaaaat
      601 acccgatagg agagcacgtc gtattgatgg cgagcgcagg atacagcgag ctgccggaag
      661 agattaccga cagcccgatg attgatcgta atgagagttt tacctttgtc accggcgtga
      721 gctggcgttt ttaattcacc ggtggatgtc ggtgcggctg gcctacgctc tcgcaccgac
      781 ttgtatgcct gataagacgt gttaagcgtc gcatcaggca atgtgctcct tacgttggat
      841 gcggcgcgag tgccttatcc aacctacgga tggcaatgct tttcctcatt cacccagatc
      901 cgcatttcgc cttcaccccg gttggcccag ctaaaccacg ggataaaggt cagagtttgc
      961 gtctggcgtt tggctggcgc gctgtcgtaa tgccacagcg gttgctgctc tggattgctc
     1021 tgttcatacc ggtaacccgg tgcctggatt aagatcttat ggctaaacaa tcccttgcct
     1081 tcaaatgtcg taaatggcgc atcggtgggc agccacagat tatgcagtga ctcgccgttg
     1141 tcggcctttt ccaggcaata caccagcggg ccgcgctgaa tcgccacttt tccggcgacg
     1201 tgacgcacca gcgggttacc gtaaacgcgg cgtaccggca tcggcaaagt cagattcagc
     1261 gtatcgccct cctgccattc gcgggtaatg tgcaaatacc ctttacgaat atcctgctcg
     1321 acctcttccc cattcaatat gatctgcggc tgtgtgcacc agtccggcag acgtaaagcc
     1381 agcgtatgac gtaccggctg gggcgattca accgcaatcg tcacctgctc ctgccacgga
     1441 tagttcccgc taacccgcag gcgcagcgtg ccattttcta ccggcacttc catgctgttt
     1501 cctgcgtaga tgttgatata caacgcatct tcacgcggcg tgtagagata atgaccaatc
     1561 gaggtcagca cgcgggcgat atttggcgga caacaagcgc agccaaacca acgctggcgg
     1621 atcggtttaa cgtgatcgta gatatggttg aatttcagcg attttggatg tacttccagt
     1681 ggattcacat agaagaaatg tttgccatcc aatgccatgc cgccgagcac agtgttgtac
     1741 agtgcgcgct ccatcacatc ggcatattga ctgtcgcctt ccatttccag cattcgtcgg
     1801 gcgaacatca tcaggccgat ggaagcacaa ctttcggcgt aaaccgtgtc attcggcaga
     1861 tcgtaatcgc tgctgaacgc ttcgccgctg ctttgtgagc cgatgccgcc ggtaatatat
     1921 aactgacgct gggccatatt gttccacagc cgcaggcagt cctgacgctt gctgtcatcg
     1981 tgacttaaac gcgcgagatg cgcgacgccg gtcatcagat agacaaaacg taccgcgtga
     2041 ccgatggcgg tttgctgttg cgcaaggggc aaatgtgcct ggctgtaggc tttgtctttc
     2101 accatccacg ccgggccgta ggtgtgccag tgcgatgtct gcccgcgctt ttcatattcc
     2161 tggtcgtaat agtgcggttg cgcaccacgc tgttcgacaa aatagttcgt cagcgccagg
     2221 tagcgcggct cttcagtcac ttcatacagg cgcattagtg ccagttcaat ttccgggtga
     2281 ccaggataac cgtgtaactt actttcatct ggaccaaata cgctgtcgat atgatcggcc
     2341 aggcggcaaa cgacccccag caagcgccgt tttcccgtgg cctggaagaa ggcgactccg
     2401 gcttcaatca gatgacctgc gcagtaaagt tcatgacact ccgccagatt gctccagcgt
     2461 tcttcgggtg cttttaccgt aaagtaagta ttgagatagc cgtcttcgca ttgggcggag
     2521 gcgatcagtt cgattacctc gtcggcggtt ttttccagtt cggcgtccgg cttctggcac
     2581 agcgaccagg ctaccgcttc cagccatttg gcgacgtcgc tgtcctgaaa caccatcccg
     2641 taaaattcac cctcctgaag tccggcggca atgcgaaagt tttcaatcgc atggctgggt
     2701 tccgcttctg ggatacgatc gttcaaggca tcccactgat aaggaatcac cacgtcgcgg
     2761 accagttgtt ggtactgacc gaggaacgga tcgctgaccg tcagtttatg cagatcgact
     2821 tccgaaatgt tcatcttgtg ctccttaagg ctgtacatga cgctgacgca ggtcggtagc
     2881 tatgcgtgac atcataggat tattgagttt gcaaaagcgg atcgcccctg ccagcagcag
     2941 gtggaatagc gcaggcagta atgtttccat cgccgcgatc ccgccaagcg aactggcggt
     3001 ctggttttcc acgccaggct ggtaggcgac aaaaataaac agcaggctga tgatcccggc
     3061 gctggaggcc caggccagct tgatgaaaaa cagattgaag gcgaagttca tgccggaaga
     3121 acgtacgtgg gttttccact cgccgtagtc atcggcaaag gccatcaatg agaagtgcag
     3181 tggcaatgtg aagccaagaa tcacgccatt accgaggatc accgccagcc acagcgtttg
     3241 ataagccggg ccggagggca ggaaccacat caacaccgcc agtgcagcga ggagcaggtt
     3301 ggtgtagtaa taaattttga cggtatcgaa acgccgcgtt aacgggctga caatcaccga
     3361 gccgataatg gaggcgaagg tgaccatggt gaagaacagc gacgtgtagc ccgtgctgcc
     3421 ttgtaagacg taggtaatga agtacatata cccaccgccg cgaatgttaa agacgttaat
     3481 cagcaggaaa gacatgacca gcatcagcag cagttggtcg ttgttccgca gcccggcaag
     3541 atgttcgcgc agggtaaatt tccccattgt ggagagcggc acccgttcac gaacccagaa
     3601 gaaacagcac aggaacatca ccacggcaat ggcgcacaat accccgacgc ccagttgata
     3661 gccgcgtgca gcgttgccct gaccgaagag agctaccatc cacggtaagc ctaccgaaac
     3721 cagaaatccc gctacgccgc agagaacgaa tcgccaggac tggcaggaga tcacttcatt
     3781 gtggcgggtg gtcatggtgt tgatccgcgc gcagtacggt acgttgatgg cggtataacc
     3841 gactgaaagg agcaggtaag taccgaacgc ccaggcgatt tttactccca tgctggcatc
     3901 tggcacggtg aacgtcagga tgccgataat gccgatggga atggcaatcc atagctgcca
     3961 cggacgaaag cgcccccatc ggctttgcgt ccggtcggca atgacgccca tcaccgggtc
     4021 ggagatggca tcaaacacgc gcagtgcaat aaacagcgtg ccaaccagcg ccggagttaa
     4081 tccaaaaacg tcggtgtaga aaaaggtgag aaaattcatg atcaggcaag ttattacggt
     4141 gccgcctgcg tcacccaggc cgtagcctat tttttcccgt acagataacg catcgtcaat
     4201 tttaccttta gcgatatcag cggtagtaat cggtgtagag gtcatagata ttcctcgtga
     4261 atagggagca atttttagtt ttattgaaat gacctgatta attctgccgt gaacgggaat
     4321 atcaacaagg cgagaaaaga gtaaaaaaag gtgacgattc cgacctgaat ttaaaagtgt
     4381 gaaatggatc aggtgaaatt aatgtttttg attaataatc aaaaggtaat gtgtttaatc
     4441 attaatgaat aagcgggaaa ttacatatca atgctcgaat tatccatgac gttaccgatt
     4501 aaagtgcaaa atggtggatt atttatttcc cgtggcgtag ggaggcattc tgcaaggcgg
     4561 ctggagtcat gggaaattat ctttgtcgaa aaaggtcgat taacgattca ggaagaagaa
     4621 cggatatttc tggtcgatgc gggagagagt ttattactct ggccgaaccg acaacatgtt
     4681 ggggtggagg attttcccgc cgatctcaaa ttttactggc tgcattttga ggttaaagcg
     4741 ccggacaacg accctcgctg gcttactcat cttagcgtgc cgcaacaaac gaaggttgtc
     4801 gatccgcagg cgctggtggc cctgtttcgc cagttcttaa gcgaacagga aaagcatcaa
     4861 cgcactccgg cgctggagtt tattgtgctg ctcattttgc agcagctcac ccgtgacgcg
     4921 cgtcttgatg aacaggccga tggcgcaggc accacccttg cctggaaagc gcagaaactg
     4981 atccgcacgc aatatcatcg cccgctttct acggcgcttc tggcccgcga gctgtactgc
     5041 aatgccgatt atcttgggcg cgtctaccgg cgggtgtttc atctgacgtt aacagaagca
     5101 atccatcgcc agcgggtgcg cgaagccgaa aagctgttaa ttagcgaagc gcgatcgctc
     5161 acggaagtgg cggcgttgtg cgggtttaat gacgttggct atttccggca gatttttcgc
     5221 aaacacaccg gtttaacgcc agcagcgtgg aagcggcgtt acagcaagga acatatcaat
     5281 tcgtagtgcc ggggcgattt cgctttcgac atcacgtctg aaacaggtgg gggagaaaaa
     5341 tctcctgaaa ccactgcgtg aaaccgcgca ggctatttct aatgaactgg gatttactgt
     5401 cagggacgac cagggcgcga tcacataacg ctttggacaa gtgccaaaac tttaacattt
     5461 ccttcgttgg atcaaagcag tagggacgcg ctctctggca ctctgctgtt ttagtgcaaa
     5521 ggagtgatca tgaaccggtt tattattgcg gatgcgacga aatgtatcgg ttgccgtacc
     5581 tgtgaagtgg cttgcgcagt gtcgcatcag gagaatcagg attgcgctgc gttgtcacca
     5641 gacgagttta tttcccgtat tcgtgtcatt aaagaccaca gctggaccac ggcagtagcc
     5701 tgtcatcagt gtgaagatgc accgtgcgcg aatgtctgcc ctgttgacgc gataagccgc
     5761 gaacatgggc atattttcgt tgaacaaaca cgttgcattg gctgtaaaag ctgtatgctg
     5821 gcttgcccgt ttggtgcgat ggaggtcgtt tcttcgcgca aaaaggcgag ggcgattaag
     5881 tgcgatctgt gctggcatcg ggagacggga ccggcctgtg ttgaagcctg cccgacaaag
     5941 gcgttgcagt gcatggatgt cgagaaagtg cagcggcatc ggctacggca gcagcctgtt
     6001 tgaaacgttt ttccggcgtt tctcaacgcc ggaatagtgc gctttgttta tgccagatgc
     6061 ggcgtaaacg ccttatccgg cccacaaatt cttgcaaatt aaatatattg cagaaatcat
     6121 gtaggcctga taagcgtagc gcatcaggca atttttagtg actttcagcc caggctcttt
     6181 ctatctcttc cgccagaatc ttcacccccg cctcaatttt ctccggctct ggtacgtagt
     6241 tcatgcgcat acattgatgc gtatgcggcc acggtttgtc cagccctggg aagaagttgt
     6301 gccccggcac catcagcacg ccgcgtgctt tcaggcgctg atagagctgc tcggtcgtaa
     6361 tgggcaaatc cttaaaccaa agccagagga aaatggctcc ttctggttta tgaattaggc
     6421 agcgatcttc cggtaaatag cggcgaatga tggcgatagt ttcctgaaca cgctggtagt
     6481 aaaacggttt gatgactgtt tcagacaggc gcagcagatc gttacgctta atcatttcac
     6541 acatcatcgc cggaccaata ccgccaggtg ccaggctgat aatgccgttc atattggtga
     6601 tggcggtgat gattttttca ttggcgatga taatgccgca gcgggagcca ggcagcccca
     6661 gcttggaaag actcatgcac agcacgatat tcggattcca tagcgggcgc gcttcactga
     6721 agatgatacc cgggaacggg acgccataag cgttatcaat caccagcgga atgccgtgtt
     6781 gattcgccag cgcgtcaagc ttcagcaact cttcgtcagt aatcacattg cctgttggat
     6841 tcgtcggccg ggagacgcaa atcatcccgg tttcttcgcc aatatgcaga tgctcaaaat
     6901 cgacgtggta tttaaactgg ccttccggca gcagttcaat attcggacgc gcagagacaa
     6961 acagatcttc ttccagtccg gcgtcagcgt agccaatgta ttccggtgcc agcgggaaca
     7021 gcactttttt gacccgacca tcggcacggc gtccggcaaa cagattaaat aagtagaaaa
     7081 acgcgctctg gctgccgttt gttagtgcaa tattctgtgc ttcgatatcc caacccaact
     7141 tctcgcgcag cattccggca agcagtgtga gtagctccgt tttcccctgt ggaccgtcgt
     7201 aattgcacag tgcatcagtc gctttgccac tttccagcat gtcggtcagt agcgtctgga
     7261 agtagtcctg catttccggg atctgcgccg gattaccgcc gccgagcata atcgcgccag
     7321 gcgtgcgtaa accgtcgttc agatcttcca tcagcagcgt aatgccggag tggcgggtaa
     7381 atttgtcacc aaaaagggag aatgtcatag cgggatatct gtcgaactct gtagcaagga
     7441 aggtaacaat aacgctacat ttgctggggt gcaaatcggt ctgttgaaga gtgagcggtg
     7501 ctttatcctg caacgctgat tagggctgac attttatcct ggttggcggc ttcccgaggt
     7561 actgacgaac gggaagccgg aaaagtcact gttgcccagc ccagatgacc agcactttat
     7621 cgtcgccatg ctcacgaaca aagccgtagc cctgcttcag cgaaagtgtc gtttgtttgc
     7681 ccgcgccaat tgcgggatgg cgggcgcgga actggctgat tttctgccag tgcgcgacgt
     7741 tggcggcaga tttaccgcta acatcctgcc agttcatatc cgaacgtgta ccttgcagcg
     7801 gatcagaacc tgtaggaccg aacggacgcg aggattcatc gccataaaag atttgtaccg
     7861 cgcctggcgc taatagtaat aactctgctg ctttgtcgcc cccttcacgg aacagacggg
     7921 tatcatgcga cgagaggtag ctcaacacgt tgaaaccctg caatttctcc gccatttgct
     7981 gccaggtcgt atccatctgc gcaatacaat cgacagcttt cgccgcctgc tcctgataat
     8041 cgaaattgat catcgcatcg aagccgtggc gatagtagtc actttgcatc acgccgtggc
     8101 cccaggcttc accggtcatc cagaaaggtt tgtcatctaa tgctttgtcg gggttagctt
     8161 ttttccattc gcgaagcgcg gcgctggctt cggttttcag ttgctgccaa gcgggcaact
     8221 caacatgttt ggcggtatcg acccgaaaac catcaatccc atagtcgcgg acccactgac
     8281 ttaaccagtg ggttaagtaa tcgcgagggg taaagccgtc gatggcttta gcgtgggtat
     8341 ccgttttgtt tttatagaac accggcagac cagaagcggt agttgattcg gttttgatat
     8401 ccggcaaaaa ggctagcgac atggtgagat cgtcgaatcc aggattgtcg taatcgccga
     8461 tatcggtacg gatccagttt tttccccacc atttatccca gcctgttttg tcgctgaaat
     8521 taatgtaatc gttaaagcta tgccaggttt gcccggcggc aggtttccag tcgctccagc
     8581 gttcacccag cgtttttttc acttcgtcac cagaaagata taacgcgcca aactgatact
     8641 cctgcatatc cgccagcgtg gcatagccgg tgtggttcat cacgacatca aagagaatac
     8701 gaataccgcg ctgatgtgcg ctatcaacca gcgtccgtag atcggcttcg ttgcccatat
     8761 tggcatcaag attcgtccag tcctgtgtgt aataaccgtg gtaggcataa tgcgggaaat
     8821 cgccttttgt accgccgccg acccagccgt gaatttgctc aaatggggcg cttatccata
     8881 aagcattaac gcccaactgc tggaggtaat ccagtttgtt ggtcaggccg cgtaaatcgc
     8941 cgccgtgaaa agtgccaatt tccgccatac cgtctttatg acgtccgtaa ctctggtcat
     9001 tactgggatc gccgttttcg aaacgatctg tcagcacaaa gtaaaccgtg gcgttatgcc
     9061 agtcgaaagg ggcagaggcg tcagtttctg cccgttccag caggagtaaa ccgttgctgg
     9121 tagcagcggg ttgtaacgtt atttgaccgt tcttcactat cgcaatttgc tggctgtaat
     9181 aatcccgtac ggcggctcct tccgggaaag tggcgctgac atccagcgtg agcggtaatc
     9241 catcccattt cgggcattca cggaccaggt ttgctaccgg ttcggcggcg ttctggatgg
     9301 aaatcatcaa tgttggcgta ccggagcggg tgtctatttg cagcgtatat ttgccgtccc
     9361 tgaacaatcg ccattgaggc ggcgtgttgc tacaaggttg cagggaaagc atctgattga
     9421 gttttatcgc atctgcaggc tgccagcact gctggtcaaa atttagcgtg agtggacgcg
     9481 tacctttggg caactgcgcg tggctgacaa atgttcccgt tccctgttcg ctaaaggcag
     9541 ggaaccccgg agaagtccag ctggcggcaa cggcgaagcc aggaaggagt gtcagaaaac
     9601 aggcggcgag tttcatggtg gatgagtcct tattgctgcg atttcagaca gtttgccagc
     9661 gataagccag acaaaactca tccctgagcg gtaagtccac aggatgagaa gcaagggtga
     9721 gcgatcgcgc gcaaaaaacg gctgaatttt gcgataaacc ccacattttc tgcgatttag
     9781 cgccaatctg aatcgttaac tcgtgacata gtttcagatt tggactattc cttggggtgc
     9841 tcttgacagc tatttttgat atgatttgag attcaactct caaatttgtg aaaaatataa
     9901 ggtgttggaa tgattaaatc cgaccaggag acctgatgat tttgactccc atacgacgat
     9961 atggggcgat gattcttatg ttactcactc tggtgttttc gagtgaggtg ttagcgaaga
    10021 cgcacacaac aacagcgagt caaaagtccc acttaactaa agctagtaat aaacaggtaa
    10081 gcagtaaaca agagtattct cgcaatagtg caaagagtag ttcacttcct gatttgcgaa
    10141 aatacccttc cggaacatca aggaaaaagg cgtttctccg gaccgtaatg ccttacatta
    10201 ccagccaaaa tgcagccatt actgcagaac gtaactggct gatttcaaaa cagtatcagg
    10261 gccaatggtc acctgctgag cgtgcgcgtc tgaaagacat cgccaaacgc tacaaggtga
    10321 agtggtctgg taatacgcga aaaatcccgt ggaatacctt gcttgaacgc gtagacatta
    10381 tccctaccag tatggtggcg acgatggctg cagcagaaag cggttgggga acgtcgaagc
    10441 tggcgcgcaa caacaacaat cttttcggca tgaaatgcat gaaaggacgt tgtaccaatg
    10501 cgccaggtaa agtgaaaggg tactcacagt ttagttctgt caaagaatcg gtgagcgcct
    10561 atgtcactaa cctcaatacg catccggctt actcttcgtt ccgtaaatcg cgtgcgcagc
    10621 tgcgtaaagc tgatcaggaa gtgactgcca cagcgatgat tcacaagctg aagggctact
    10681 cgaccaaagg gaagagttat aacaactacc tgttcgcaat gtaccaggat aaccaacggt
    10741 taatcgcggc gcatatgtaa ttgtatttcc tcgagcctta tccgacttgt cggtcggata
    10801 aggcttttga tcgcatcagg tggttgtgct gagttacctg atgcgacgcc gacaattctc
    10861 atcatcgcta caacatgacc tcgctattta catcgcgata ctcttttggc gtcgtgtcat
    10921 atgctttttt aaaaacagag tagaaatatt gcagcgatgg ataaccgcac atttgcgata
    10981 tctcattgat cgacaaggtg gttgaaatca gcagactgcg cgctttctcc agcttctcag
    11041 catgaatcat ggcatggatg gtttcaccca cctcttcttt aaaacgcttc tcaagattgg
    11101 agcgcgagat cccgaccgca tcgagtacct gatccacttt aatcccttta caggcgtgat
    11161 tacgaatgta atgcatggcc tgaataacgg cgggatcggt cagcgaacgg taatctgttg
    11221 agcgccgttc aatgacgcga actggaggga ccaaaatccg ctgtagcggc atttcttctt
    11281 tatctaataa tcgatgcaac agttttgccg cctgatagcc catttgccgc gcgccctgag
    11341 cgaccgaaga aagggcgaca cgcgacagat agcgggtcag ttcttcgtta tcgatgccaa
    11401 tcacgcataa tttttccggt acgggaatgt gtagatgttc acatacttgc agaatatgcc
    11461 gcgcccgggc gtcagtaacg gcaataatcc cggtttgcgg tggcagtgtt tgtagccagt
    11521 ctgccagccg attttgcgcg tgttgccagt tctctggtgc ggtttctaac ccctgataaa
    11581 ccactccgcg atacttttct tcggcgacaa gctgacgaaa tgcatattcg cgttcagtgg
    11641 cccaacgttt gccgcttgat tccggaagac cataaaaagc aaagcggtta acgcctttct
    11701 cttttaaatg caaaaatgcg ctttcaacca gcgcatagtt atcggtggca atgtaatgaa
    11761 cgggtgggta actttctgca aggtgatacg agccgccaac cccaacaatg gggacgtcga
    11821 catcagccag cgcttgctcg atctgtttgt cgtcgaagtc ggcaatgacg ccatctccta
    11881 accagtcctt gattttatca atgcgggcgc ggaaatcttc ttcaatgaaa atatcccatt
    11941 ccgattgtga cgcctgtaaa tattccccta cgccttctac tacctgccgg tcataggctt
    12001 tattggcatt gaacagtaat gtgatgcggt gacgtttagt aaacatggtt cttttcctgc
    12061 tgaatcatgc aaaaactcaa aaccggtaat acgttaccgg ctttgagaaa atttttatca
    12121 aaatcaagaa cggcgtttgg ttgcggagtc catccatact gccagcaaca gaatcgcacc
    12181 tttaacgata tactgccaga aggtcggtac atccatcata ctcataccgt tatccagtga
    12241 agccatgatg aatgccccca ttaccgctcc agccacgctc ccgacgccgc ctgccagact
    12301 ggtgccgcca atcacgcatg ctgcaattgc gtccagctcg gcgatattcc ccgcagaagg
    12361 tgaaccagcg ccaagtcgag aactgaggat taatccggcg atggctacca ttaatccgtt
    12421 aatagcgaac acggcaagtt tggtgcgttc aacgttaatt ccggagagac gtgctgcttc
    12481 cagattgccg ccgatggcat aaatgcgtcg tccaaatgct gtccgcgttg ccataaacat
    12541 tccgccgagt aacagcaacg tcagcagcag aacaggagta ggaacgccac ggtaatcatt
    12601 caacagccag attgcgccta atacgatgat agcggttaaa gcctggcgac cgactactgc
    12661 ggtagaggct ggagactgca aacccaaagc ctgacggcgc attcttccgc gccattgcca
    12721 gccaacaaaa gccattaagc caagcgcgcc aatgatgaag ccggtgctgg cggggagata
    12781 gctttgccca atttgtgaca tcgcggcgct ggtgggggat acagtcgtgc cgttggtgat
    12841 gccaatgagt atgccgcgaa atgccaacat gcccgcgaga gtgacaataa atgaagggac
    12901 tttgcggtac gcgacccacc atccgttcca ggcaccgaga agcagtccca gaaccaacgt
    12961 cacaatgatg gtaagtggca aaggccagcc tagccagacg tcacaaatcg ccgcgacgcc
    13021 acctaatagc cccatcattg agccgacgga aaggtcgatt tcagcagaaa ttatgacgaa
    13081 caccattcct accgcgagga tgccggtaat cgcggtctgg cgtaacaggt tggagacgtt
    13141 acgggcgctt aagtaggcac catcggtggt ccaggtaaag aacagcatga ttgcgatgat
    13201 agctgcaatc atcacgaaga cctgcaaatt cagtgatttc agcccggaga agccaccgga
    13261 tgtcggtacg gccaatttca cttcagacgg attgcttttc gacatgatgt tcgctcctca
    13321 atgcggcttc catcacctgc tcctgagtca ggttatgatt tatcaggttg gcttttagtt
    13381 tcccttcatg cattactagt acacgatcgc taaggccgag cacttcaggt aattcagaag
    13441 agatgacaat aacggcaata ccctgctgga cgagttggtt aattaatttg tagatctcgt
    13501 atttcgcgcc aatatcgata cccctggtgg gttcatcaag aatgagaatg cgcgggttaa
    13561 gtaacagaca gcgagcgagg atcgcttttt gctgattgcc gccgctcaaa cgtccaatag
    13621 caaggtcggg ggacgacgtt ttaactttga gttgctggat tgattccaga atacattttt
    13681 gctctgccgc gtcatcaagc tggctaatgc caccggtaaa tttattgagt gcggcgaggg
    13741 taatattttt accaaccgcc attaccggaa cgatgccgtc gcgctttctg tcttcgggga
    13801 ccatcgcaat cccctgggcg atggcttgct gacagttacg aatatctacc tgtttgccat
    13861 caatataaat ttttccttcc cattgtccgg gccacacacc aaacaggcac tgaatggtct
    13921 cggtacgtcc ggcaccaacg agtccggcaa tacccagtat ttcgccacgt ttcagggaaa
    13981 acgagacatc attaactcgt ttaatatgac gattaaccgg atgccatgcc gtcagatgtt
    14041 caatacgtaa tatttcatct ccggtggtat gtggttcatt agggtaaagc gcggttaact
    14101 ctcgcccgac catcatggtg ataatatcgt cttcactcat tccggcagca tcacgcgtac
    14161 caatgtgctg tccgtcgcga ataacgcaaa tcgtatcgga aatcgctttg acttcgttga
    14221 gtttgtgcga aatataaata caggcgatac cgtgctgttg tagatcgcga ataatatcca
    14281 gtaaaaccga cgtttcctgc tcagttaatg aggctgtcgg ttcatcgaga attaacaagc
    14341 gcacctgttt attaagtgcc ttggcaattt caaccagttg ttgttgccca agccctaaat
    14401 cgccaacgcg ggtatcaggt gaaatggata aactgacctg tgcgagcagc ttctgacagc
    14461 gtagcgtcat caggtcataa tccataatgc cattgtgggt tatttcgtta cccaggaaga
    14521 tattttccag cacggtcaat tctttcacca gggccaattc ctggtgaatg atggcgatac
    14581 ctttgcgttc ggtatcgcgg atgtgactcg cctgaatctc ttctcccgca aaaataattt
    14641 cgccttcgta ggagccatgg ggataaatac cacacagcac tttcatcagc gttgatttac
    14701 cagacccatt ttccccacag agtgagacga tttcgccagc attcaaccgc aagctgacgt
    14761 tatcaatcgc cttcacactg ccgaaggttt tggtaatgtt cttcatttca agtagataag
    14821 gcatagcgac tccacctaag ccaattcatt cacgcggcat ggagagaaat cacgcccccg
    14881 ctccgcgctg gggcgtaacg cttacagctc gctctctttg tggaatccgt ctttaattac
    14941 cgtatctttg atgttgtttt tattcacatc gatcggtgtc aggaggcggg aggggacatc
    15001 tttcaggcca ttatttagtg aagtatctgc ttttggctcc tgaccattgc ccaattcaac
    15061 ggcaatttct gcggcagtat ttgccagcaa tgtaataggt ttatataccg tcatagtttg
    15121 cgtaccggca gcaatacgtt taatacctgc gagatccgca tcctggccag aaattgctac
    15181 tttccctgat aaaccttgcg cgcttaatgc ctgaattgcc ccacctgcgg tggcatcgtt
    15241 tgaggcaact acagcatcaa ttttgttatt attggcggtt agcgcgtttt ccataatttt
    15301 caatgcgttt tccggtaacc agccatcaac ccattggtca ccaacgactt taatttttcc
    15361 ggaatcaacg taaggttgta acactttcat ttgtccggcg cggaacagct tagcgttgtt
    15421 atctaccggc gagccgccca tcaggaagta attaccttgc ggaacaatat cgaccagggc
    15481 ttttgcctgc agttcaccga ctttttcgtt atcgaaagaa atataaaaat cgatatccgc
    15541 atcgttaatc atacggtcgt aagctaatac tttaatgcct tcttgtttgg cttcttttac
    15601 aacgttactt aatacctgac cgttatacgg aataatgaca agaacatcga caccccggtt
    15661 tatcatgttt tcaatctgcg acatttgtgt ttcttcattg ccatttgcag actgtacaaa
    15721 tactttcgcg ccgagagatt ctgccttttt cacaaagata tctcgatctt tttgccagcg
    15781 ttcaagacgg agatcatcaa tcgccatacc tattttgact tctttggcgt gtgcagcaac
    15841 gttggtaagc aggagtgagg tgcaaagggt gagtagaatg ttctttattt tcatggtgta
    15901 gggccttctg tagttagagg acagctttaa taagtaacaa tcaccgcgat aaacgtaacc
    15961 aatttttagc aactaaacag gggaaaacaa ttacagattt ttatctttcg attacgattt
    16021 ttggtttatt tcttgattta tgaccgagat cttacttttg ttgcgcaatt gtacttattg
    16081 catttttctc ttcgaggaat tacccagttt catcattcca ttttattttg cgagcgagcg
    16141 cacacttgtg aattatctca atagcagtgt gaaataacat aattgagcaa ctgaaaggga
    16201 gtgcccaata ttacgacatc atccatcacc cgcggcatta cctgattatg gagttcaata
    16261 tgcaagccta ttttgaccag ctcgatcgcg ttcgttatga aggctcaaaa tcctcaaacc
    16321 cgttagcatt ccgtcactac aatcccgacg aactggtgtt gggcaagcgt atggaagagc
    16381 acttgcgatt tgccgcctgc tactggcaca ccttctgctg gaacggggcg gatatgtttg
    16441 gtgtgggggc gtttaatcgt ccgtggcagc agcctggtga ggcactggcg ttggcgaagc
    16501 gtaaagcaga tgtcgcattt gagtttttcc acaagttaca tgtgccattt tattgcttcc
    16561 acgatgtgga tgtttctcct gagggcgcgt cgttaaaaga gtacatcaat aattttgcgc
    16621 aaatggttga tgttctggca ggcaagcaag aagagagcgg cgtgaagctg ctgtggggaa
    16681 ccgctaactg ctttacaaac cctcgctatg gcgcgggtgc ggcgacgaac ccagatccag
    16741 aagtcttcag ctgggcggca acgcaagttg ttacagcgat ggaagcaacc cataaattgg
    16801 gtggtgaaaa ctatgtcctg tggggcggtc gtgaaggtta cgaaacgctg ttaaataccg
    16861 atttgcgtca ggagcgtgaa caactgggcc gctttatgca aatggtggtt gagcataaac
    16921 ataaaatcgg cttccagggc acgttgctta tcgaaccgaa accgcaagaa ccgactaaac
    16981 atcaatatga ttacgatgct gcgacagtct atggcttcct gaaacagttt ggtctggaaa
    17041 aagagattaa actgaacatt gaagctaacc acgcgacgct ggcaggtcac tctttccatc
    17101 atgaaatagc caccgccatt gcgcttggcc tgttcggttc tgtcgacgcc aaccgtggcg
    17161 atgcgcaact gggctgggac accgaccagt tcccgaacag tgtggaagag aatgcgctgg
    17221 tgatgtatga aattctcaaa gcaggcggtt tcaccaccgg tggtctgaac ttcgatgcca
    17281 aagtacgtcg tcaaagtact gataaatatg atctgtttta cggtcatatc ggcgcgatgg
    17341 atacgatggc actggcgctg aaaattgcag cgtgcatgat tgaagatggc gagctggata
    17401 aacgcatcgc gcagcgttat tccggctgga atagcgaatt gggccagcaa atcctgaaag
    17461 gccaaatgtc actggcagat ttagccaaat atgctcagga acataatttg tctccggtgc
    17521 atcagagtgg tcgccaggag caactggaaa atctggtaaa tcattatctg ttcgacaaat
    17581 aacggctaac tgtgcagtcc gttggcccgg ttatcggtag cgataccggg cattttttta
    17641 aggaacgatc gatatgtata tcgggataga tcttggcacc tcgggcgtaa aagttatttt
    17701 gctcaacgag cagggtgagg tggttgcttc gcaaacggaa aagctgaccg tttcgcgccc
    17761 gcatccactc tggtcggaac aagacccgga acagtggtgg caggcaactg atcgcgcaat
    17821 gaaagctctg ggcgatcagc attctctgca ggacgttaaa gcattgggta ttgccggcca
    17881 gatgcacgga gcaaccttac tggatgctca acaacgggta ttgcgccctg ccattttgtg
    17941 gaacgacggg cgctgtgcgc aagagtgcgc tttgctggaa gcgcgagttc cgctatcgcg
    18001 ggtgattacc ggcaacctga tgatgcccgg atttactgcg cctaaattgc tatgggttca
    18061 gcggcatgag ccggagatat tccgtcaaat cgacaaagta ttattaccga aagattactt
    18121 gcgtctgcgt atgacggggg agtttgccag cgatatgtct gacgcagctg gcaccatgtg
    18181 gctggatgtc gcaaagcgtg actggagtga cgtcatgctg caggcttgcg acttatctcg
    18241 tgaccagatg cccgcattat acgaaggcag cgaaattact ggtgctttgt tacctgaagt
    18301 tgcgaaagcg tggggtatgg cgacggtgcc agttgtcgca ggcggtggcg acaatgcagc
    18361 tggtgcagtt ggtgtgggaa tggttgatgc taatcaggca atgttatcgc tggggacgtc
    18421 gggggtctat tttgctgtca gcgaagggtt cttaagcaag ccagaaagcg ccgtacacag
    18481 cttttgccat gcgctaccgc agcgttggca tttaatgtct gtaatgctga gtgcagcgtc
    18541 gtgtctggat tgggccgcga aattaaccgg cctgagcaat gtcccagctt taatcgctgc
    18601 agctcaacag gctgatgaaa gtgccgagcc agtttggttt ctgccttatc tttccggcga
    18661 gcgtacacca cacaataatc cccaggcgaa aggggttttc tttggtttga ctcatcaaca
    18721 tggccccaat gaactggcgc gagcagtgct ggaaggcgtg ggttatgcgc tggcagatgg
    18781 catggatgtc gtgcatgcct gcggcattaa accgcaaagt gttacgttga ttggtggcgg
    18841 ggcgcgtagt gagtactggc gtcagatgct ggcggatatc agcggtcagc agctcgatta
    18901 ccgtacgggg ggggatgtgg ggccagcact gggcgcagca aggctggcgc agattgcggc
    18961 gaatccagag aaatcgctca ttgagttgtt gccgcaacta ccgttagaac agtcgcatct
    19021 accagatgcg cagcgttatg ccgcttatca gccacgacgt gaaacattcc gtcgcctcta
    19081 tcagcaactt ctgccattaa tggcgtaaac gttatcccct gcctgaccgg gtgggggata
    19141 attcacatct atatatctca gcaattaatt aatatttagt atgaatttat tctgaaaatc
    19201 atttgttaat ggcatttttc agttttgtct ttcgttggtt actcgtaatg tatcgctggt
    19261 agatatggag atcgttatga aaacctcaaa aactgtggca aaactattat ttgttgtcgg
    19321 ggcgctggtt tatctggttg ggctatggat ctcatgccca ttgttgagtg gaaaaggcta
    19381 ttttcttggc gtgttaatga cagcaacttt tggcaactat gcatatcttc gcgcagaaaa
    19441 actcgggcaa ctggataatt tttttaccca tatttgccag ttagttgcgt taatcactat
    19501 cggtctcttg tttatcggtg ttttaaatgc acctatcaat gcttatgaaa tggtgatcta
    19561 ccccatcgcc ttttttgtct gcttgtttgg tcaaatgcgt ttgtttcgct cggtatgagc
    19621 aacataaagc tcttacacat tcaggaatga aaggaatact gtgatggaca acaaaatatc
    19681 aacctattca ccggccttta gtattgtgtc atggatagct ctcgttggtg gtatcgttac
    19741 ctatctgtta gggctatgga atgcagagat gcagttaaat gaaaaaggat attattttgc
    19801 cgtactggta ttaggactgt tttctgcggc gtcttatcaa aagaccgttc gggacaagta
    19861 tgaaggcata ccgaccactt ccatttatta tatgacctgc ctgactgtct ttattatctc
    19921 tgttgcatta ctgatggtag gtctgtggaa tgcgacatta ttactcagcg aaaaaggttt
    19981 ttatggactg gctttcttct taagcttgtt tggtgcagta gcggtgcaga aaaatattcg
    20041 tgatgccgga ataaacccac caaaagaaac acaggttacc caggaagaat acagcgaata
    20101 actcacgtaa gcccggtcag tccaatgtga ccgggctttt acttaactca ctaatctgtt
    20161 tctgtcgatt cgttgtacca gcatagaaag taacaaactc gctgccaacg tcgcgcaaaa
    20221 aatccaaata atatccagta ttggccaatt tttaagctca attccccggg tgcgcagcgc
    20281 atggataatc aaggcgtgga atccgtatat acccaacgaa tggcgggaga ttaagccaag
    20341 tccgcgaatg gtacgcgtat ccagcgtgtt tttaaccaga gtcaatagcg cgattgcgca
    20401 gataaaaacc atcggcccac agtaaggata ccaggtatcg gcaaaatttc cgcgccactg
    20461 caattcatat aatgtcccgc gagagataat aaaaactccc gtcgcaaaca gtgcggcgct
    20521 cacccacgac agtgctttat gctgtgtgtc catcatccct aaagcgcggc ccaacatgcc
    20581 atacagaatg tagtaaaaag tatcgccatt gatatataag ttaattggca gccattcaaa
    20641 accgtcgatt ttctgcggca cggtgtttgg gttagcgatg ataccaatca ccaccattag
    20701 caccagcaac atttttccgc cgacgttctt cacctgaatc agcggtgaaa ccagataaat
    20761 caccgcaatc gcgaagaaaa accataagtg gtaaaacact ggcttttgca gcaggttttt
    20821 cagcgctaac tccacattaa tggaggtaaa cagtgcaatg tagagcagcg cgattgcgct
    20881 ataaaaaaac agacataagc cgatacgcaa gaaatggcgc ggctgggcgc tgcgttcgcc
    20941 aaaaaagaga tagccggaaa tcatgaaaaa tagcggcacg ctgacacgag aggcagagtt
    21001 cagaacattg gcgatatccc aggtgacggg gctaacacta tgagcattgg tcacatacca
    21061 ggtagtggtg tgaatcatca ccaccattaa acacgctatc cctcgcaggt tatcaatcca
    21121 gtaaattttg ggctgcatct gtgtctctgt atctggttaa aaaaagtctg accgataaat
    21181 cattcagttg gcgcactgga ataatctgag ttttaacact acagcttata gaggcttaag
    21241 gaaattcgta agatatcagc cactataccg atataaataa taagactcac ctgcaaacca
    21301 gacggtaatt taatgatgat gaactctttc tttccggcaa tggcgcttat ggtgctagtg
    21361 ggttgttcta caccgtcacc cgtgcagaaa gcacaacggg taaaggttga tcctctgcgt
    21421 tcgttgaata tggaagcgtt atgcaaggat caggcggcaa aacgttataa caccggcgag
    21481 caaaaaatcg acgtcaccgc cttcgaacag ttccagggaa gctatgaaat gcgcggttat
    21541 accttccgta aagagcagtt tgtctgttct tttgacgcag atggccattt tttgcatctt
    21601 tccatgcgtt aagccctgct ttttcccgtt tcatactgta tatcttccat ccagcgggta
    21661 tactgatccc ttcctttaaa tccacacgta tccagcacga aataatatgc aaaagtttga
    21721 taccaggacc ttccagggct tgatcctgac cttacaggat tactgggctc gccagggctg
    21781 caccattgtt caaccattgg acatggaagt cggcgcggga acctctcacc caatgacctg
    21841 tctgcgcgcg ctggggccag aaccgatggc ggctgcttat gttcagcctt ctcgtcgccc
    21901 gaccgatggt cgctacggcg aaaaccccaa ccgtttacag cactactatc agttccaggt
    21961 ggtcattaag ccatcgccgg acaatattca ggagctgtac cttggttctc tgaaagagct
    22021 gggcatggac ccgactatcc acgatatccg tttcgtggaa gataactggg aaaacccgac
    22081 gctgggtgcc tggggactgg gctgggaagt gtggctaaac ggcatggaag tgacgcagtt
    22141 cacttacttc cagcaggttg gtggtctgga gtgtaaaccg gttaccggcg agatcaccta
    22201 cggtctggaa cgtctggcca tgtacattca gggcgtagac agcgtttacg acctggtctg
    22261 gagcgacggc ccgctgggta aaaccaccta cggcgacgtg ttccatcaga acgaagtgga
    22321 gcagtccact tacaacttcg aatacgcgga tgtggacttc ctgttcacct gctttgagca
    22381 gtacgagaaa gaagcgcagc agctgctggc gctggaaaat ccgctgccgc tgccagctta
    22441 cgagcgtatt ctgaaagccg cccacagctt caacctgctg gatgcgcgta aagccatctc
    22501 cgtcaccgag cgtcagcgct acattctgcg cattcgcacc ctgaccaaag cagtggcaga
    22561 agcatattac gcttcccgtg aagccctagg cttcccgatg tgcaacaaag ataagtaaga
    22621 ggcggctatg tctgagaaaa cttttctggt ggaaatcggc actgaagagc tgccaccaaa
    22681 agcactgcgc agcctggcag agtcctttgc tgcgaacttt actgcggagc tggataacgc
    22741 aggcctcgca cacggcaccg ttcaatggtt tgctgctccg cgtcgtctgg cgctgaaagt
    22801 agctaacctg gcggaagcgc aaccggatcg tgaaatcgaa aaacgcggcc cggcgattgc
    22861 ccaggcgttc gacgctgaag gcaaaccgag caaagcggca gaaggttggg cgcgtggttg
    22921 cggtattacc gttgaccagg ctgagcgtct gactaccgat aaaggcgaat ggctgctgta
    22981 tcgcgcccat gtgaagggcg aaagcactga agcactgctg ccgaatatgg ttgcgacttc
    23041 gctggcgaaa ctgccgattc cgaaactgat gcgctggggt gcaagcgacg tgcacttcgt
    23101 gcgtccggtg cacaccgtga ccctgctgct gggcgacaaa gtcattcctg caaccattct
    23161 gggcattcag tccgatcgcg tgattcgcgg ccaccgcttt atgggcgagc cggaattcac
    23221 catcgataac gccgatcagt atccggaaat tctgcgtgag cgtgggaaag tcatcgccga
    23281 ttacgaagaa cgtaaggcga agattaaagc cgatgccgaa gaagcagcgc gtaagattgg
    23341 cggtaacgct gacttaagcg aaagcctgct ggaagaagtg gcttcgctgg tggagtggcc
    23401 ggtcgttcta accgcgaaat ttgaagagaa attcctcgcg gtgccgtctg aagcactggt
    23461 ttacaccatg aaaggtgacc agaagtactt cccggtgtat gcgaacgacg gcaaactgct
    23521 gccgaacttt atcttcgttg ccaacatcga atcgaaagat ccgcagcaaa ttatctctgg
    23581 taacgagaaa gtcgttcgtc cacgtctggc tgatgccgag ttcttcttca acaccgaccg
    23641 taaaaaacgt ctggaagata acctgccgcg cctgcaaacc gtgctgttcc agcaacagct
    23701 gggtacgctg cgtgacaaga ctgaccgcat ccaggcgctg gctggctgga tcgccgaaca
    23761 gattggcgct gacgttaacc acgcaacccg tgcgggcctg ctgtctaagt gtgacctgat
    23821 gaccaacatg gtcttcgagt tcaccgacac ccaaggcgtt atggggatgc actacgcgcg
    23881 tcacgatggc gaagcggaag acgtagccgt cgcgctgaat gagcagtatc agccgcgctt
    23941 tgccggtgat gacctgccgt ccaacccggt agcttgtgcg ctggcgattg ctgataagat
    24001 ggatactctg gcgggtatct tcggtatcgg tcagcatccg aaaggcgaca aagacccgtt
    24061 tgcgctgcgt cgtgccgcac ttggcgtgct gcgaattatc gttgagaaga acctcaacct
    24121 tgatctgcaa acgctgaccg aagaagcggt gcgtctgtat ggcgataagc tgactaatgc
    24181 caacgtagtt gatgatgtta tcgactttat gctcggtcgc ttccgcgcct ggtatcagga
    24241 cgaaggttat accgttgaca ccatccaggc ggtactggcg cgtcgtccga ctcgtccggc
    24301 tgatttcgat gcccgcatga aagcggtatc gcacttccgt accctggaag cagctgcagc
    24361 actggcggcg gcgaacaaac gtgtatctaa catcctggcg aaatctgacg aagtgctgag
    24421 cgatcgcgtg aatgcctcta ctctgaaaga gccggaagaa attaaactgg cgatgcaggt
    24481 tgtggtgcta cgtgacaagc tggagccgta ctttgcggaa ggtcgttacc aggatgcgct
    24541 ggtcgaactg gcagagctgc gtgaaccagt cgatgccttc ttcgataaag tgatggtcat
    24601 ggttgatgac aaagaattgc gtatcaaccg tctgaccatg ctggagaaac tgcgcgaact
    24661 gttcctgcgc gttgcggata tttcgctgtt gcagtaataa cgccgttatt aaaaagcctg
    24721 ccatctggca ggcttttttt atcgctaaat aatacagcaa ccttta
//
LOCUS       DAZFKY010000058        24715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFKY010000058 DAZFKY010000000
VERSION     DAZFKY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..550
                     /locus_tag="ACHKCL_002065"
     CDS             <1..550
                     /locus_tag="ACHKCL_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO5763033.1"
                     /translation="YNDIQTYDEQVNASWSEVLSQYQRRADLIPNLVASIKGYSSHER
                     DVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPELK
                     SQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKNYL
                     PDDVAAVSKAPTIDFSQNANAH"
     gene            564..1238
                     /locus_tag="ACHKCL_002066"
     CDS             564..1238
                     /locus_tag="ACHKCL_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5763034.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            1472..2620
                     /locus_tag="ACHKCL_002067"
     CDS             1472..2620
                     /locus_tag="ACHKCL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5763035.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            2617..3531
                     /locus_tag="ACHKCL_002068"
     CDS             2617..3531
                     /locus_tag="ACHKCL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5763036.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3591..4889)
                     /gene="eno"
                     /locus_tag="ACHKCL_002069"
     CDS             complement(3591..4889)
                     /gene="eno"
                     /locus_tag="ACHKCL_002069"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5763037.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4977..6614)
                     /gene="pyrG"
                     /locus_tag="ACHKCL_002070"
     CDS             complement(4977..6614)
                     /gene="pyrG"
                     /locus_tag="ACHKCL_002070"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5763038.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6842..7633)
                     /gene="mazG"
                     /locus_tag="ACHKCL_002071"
     CDS             complement(6842..7633)
                     /gene="mazG"
                     /locus_tag="ACHKCL_002071"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5763039.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7704..8039)
                     /gene="mazF"
                     /locus_tag="ACHKCL_002072"
     CDS             complement(7704..8039)
                     /gene="mazF"
                     /locus_tag="ACHKCL_002072"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5763040.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8039..8287)
                     /gene="mazE"
                     /locus_tag="ACHKCL_002073"
     CDS             complement(8039..8287)
                     /gene="mazE"
                     /locus_tag="ACHKCL_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5763041.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8365..10599)
                     /gene="relA"
                     /locus_tag="ACHKCL_002074"
     CDS             complement(8365..10599)
                     /gene="relA"
                     /locus_tag="ACHKCL_002074"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5763042.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10647..11948)
                     /gene="rlmD"
                     /locus_tag="ACHKCL_002075"
     CDS             complement(10647..11948)
                     /gene="rlmD"
                     /locus_tag="ACHKCL_002075"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5763043.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12005..14761
                     /gene="barA"
                     /locus_tag="ACHKCL_002076"
     CDS             12005..14761
                     /gene="barA"
                     /locus_tag="ACHKCL_002076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5763044.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14994..16334)
                     /gene="gudD"
                     /locus_tag="ACHKCL_002077"
     CDS             complement(14994..16334)
                     /gene="gudD"
                     /locus_tag="ACHKCL_002077"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5763045.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16355..17695)
                     /gene="gudX"
                     /locus_tag="ACHKCL_002078"
                     /pseudo
     CDS             complement(16355..17695)
                     /gene="gudX"
                     /locus_tag="ACHKCL_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(17697..19049)
                     /gene="gudP"
                     /locus_tag="ACHKCL_002079"
     CDS             complement(17697..19049)
                     /gene="gudP"
                     /locus_tag="ACHKCL_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5763046.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(19484..19933)
                     /gene="yqcA"
                     /locus_tag="ACHKCL_002080"
     CDS             complement(19484..19933)
                     /gene="yqcA"
                     /locus_tag="ACHKCL_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5763047.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19951..20733)
                     /gene="truC"
                     /locus_tag="ACHKCL_002081"
     CDS             complement(19951..20733)
                     /gene="truC"
                     /locus_tag="ACHKCL_002081"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5763048.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(20733..21062)
                     /gene="yqcC"
                     /locus_tag="ACHKCL_002082"
     CDS             complement(20733..21062)
                     /gene="yqcC"
                     /locus_tag="ACHKCL_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5763049.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21684..22229)
                     /gene="syd"
                     /locus_tag="ACHKCL_002083"
     CDS             complement(21684..22229)
                     /gene="syd"
                     /locus_tag="ACHKCL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5763050.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            22297..23145
                     /gene="queF"
                     /locus_tag="ACHKCL_002084"
     CDS             22297..23145
                     /gene="queF"
                     /locus_tag="ACHKCL_002084"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5763051.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23257..24621
                     /gene="ppnN"
                     /locus_tag="ACHKCL_002085"
     CDS             23257..24621
                     /gene="ppnN"
                     /locus_tag="ACHKCL_002085"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5763052.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6078 a         6485 c         5940 g         6212 t
ORIGIN      
        1 atataacgat attcaaacct atgatgagca ggttaacgcc tcctggtctg aggtattgag
       61 tcaatatcaa cgtcgcgccg atcttattcc taatcttgtc gccagcatta aaggttattc
      121 cagccatgaa cgagatgtac tggaagccgt aacattggct cgtagccagg ctaatcgcgc
      181 cagtagcgat cttcagcaaa ctccgggaga tgaacaaaaa ttacaagcct ggcaacaggc
      241 tcaagcgcag gtgactcgca ctctcggtca gttaacgata attagtgagc gttaccctga
      301 gttaaaatct caggaactct atcaaaattt aatggtacag cttgaaggaa gtgaaaatcg
      361 gattgccgtt gcgcgaggaa gatatattaa agccattgaa caatataatg tcactatccg
      421 caaattcccc gctgtattaa ccgcaaaggt catggattac acaccaaaga aaaattattt
      481 gccggatgat gtcgccgcag taagtaaagc cccaacaata gattttagcc agaatgctaa
      541 tgctcattaa gcggggaagt tctatgcgaa attttatctt ccttatggct ttcttctgtt
      601 catctgtgtt tgccacacaa attccagtac ctgaatcacc caagtatgtg aatgacttaa
      661 ccggcacatt aacaaacagc gaagtaaaca ccctgaccaa ccagattaaa gccctgacgc
      721 aaaaaaacta cgcacagttg gtcgtgttag tggttgaaac aacaggcgat gaaaccatcg
      781 agcagtacgc gacgcgggta ttcgatagct ggaaaccggg tgataaagac cgcgatgatg
      841 gagttctact gttagtcgcc tggcaggatc ataccgtgcg tattgaaatc ggttacggac
      901 tggaaggtat tattacggat gcgcagtccg ggaaaattat tcgcaacagt attattcctg
      961 catttaaaaa aggcgacctg gctggtggtt tgcaaaaggg aatcagcgat attgaaagtc
     1021 gtctgacggg gaataattta gcaacgataa caccgaccga tcaccctttg ccctttagcg
     1081 gttggtgggc attgttagtc tgggcgatag tactgacgtt tatttctgcc agaggatata
     1141 tcaaaacgct gggagtaatt tgcttcgcag ctattgtact ggcctttgtt ttacctatag
     1201 caggcttcag cggaagctgg ggagtactcg caacatgact ctgcttcgcc acaccgttcc
     1261 tggctgtcgc catcgctttt acccccttcg gcaaaaaagt tcgggatttg atgcgtgatg
     1321 ccaaccagcc atcacgccat acaagaagca attcttcctc ccactattca tcatcgtcat
     1381 cctcttcatt cgacaatgac aattttagcg gtggtggcgg ttcatctggt ggcggtggcg
     1441 cttccgggcg ctggtaacaa aaggaattga tatgcgtatt tttctcttac ttctgatgct
     1501 atgcggtttt caggtattcg ctgaacgatt gccttcagtg actgacttaa ccgggacatt
     1561 aacgacagaa gaacaaactg ctttaactca acaattacag actctcgagc agcaaaacca
     1621 cttccaggtg gccgtgttgg ttacgccgac cacagggggc aaaaacatta aacttgcagc
     1681 ctcgcagaga tacggggtat atcactggaa acccgttgga gagaagcgat atggcgaagg
     1741 tatcttaatt ctcgtcgtat ggccggaagg gctggcgtca atgaagattg gacatggact
     1801 ggaagagatg ttgccgccgg aacaagctgc gcaaattgtt cgttaccata tgcagccaga
     1861 attcgaaaaa aacaatctct ttgctggatt aacgggcgga attgaaagca tcgcacagtt
     1921 tacccatatc aaagcgaacc ttagcccttt ggatgcttta gccaatcatc tgtttgctaa
     1981 tccgcaacgt tcgctgccat gtcttgcctg gacactgctg atgatcgttg ccatcgttgt
     2041 cctctggcta tttgccagcc gcccacggcg tggcatatgg atgatatcga tgattacccc
     2101 tatggtctgg tttttctcct ttcaggacga tgtcatcatt cggcgggtat cggtcgtttg
     2161 ttttagttta ttgttagcaa caacctgcaa gcagcgtctc gcggtagttt ttaatatgtg
     2221 ccgcgtattt cctatgatgc tgaccccaaa aaataaaaac gctaaagtga aaaaacaaaa
     2281 gaagactccg caggttcgtg aatcaggacg aaattcattg tttgtcgtga tgctgataat
     2341 tattgtggga tggtgcctgg tgttcgccag taataaagat attgcattca tctcaacaat
     2401 tattgggtta attgatcatt ttatcggccc aataaccatt gctggcatcg ttctgctgat
     2461 cattgtggcc aaatttactg gcaatcttaa gctggggtca ggagaaaaaa gcaacaggaa
     2521 aacggggaat cgtaatgctc attctcgctc ttcgtcgaga aattctttcc gcggtggtgg
     2581 cggttcttca ggaggcggcg gttcatccgt ccgttggtga tcgtaatgcg cttaatcttt
     2641 attctattca ctttgtggtg ccttcctggg cttgcccagc aaattgctgt gccggaactg
     2701 cgccaacagg tgaccgatat tactggtacg ttaagcactt ctgaacaaca atcactgaca
     2761 cagcaactgc aggatattac acataaaaca cgggcgcagg tcgctgtatt agtagtgccg
     2821 tccaccggag acgattccat tgaacaatat gctacaaggg tttttgatag ctggaaactg
     2881 ggagataagc agagaaacga tggtatttta cttctggttg cctgggagga tcatgctgtt
     2941 cgtattgagg ttggctacgg gctggaaggg gtggttaccg acctgcaagc ggcgaagatt
     3001 atcagggata tattgatccc tgccttcaaa agtgatgacc tgatgggtgg attaacactg
     3061 gcaagcgaaa atatcggcgc gcttctgttg aatggtgaat taccggaaga caggggggat
     3121 tattacagta tcaatccccc tattccatta tcgcttgctg taattatatt gctggcggta
     3181 ctttcatatt ttattgtttt taccgatccg tcgaatttac cctggatcac tctcactggc
     3241 gccatttacg ggatggtatt cctctatgtt gccgagccag gaccatggac caatttaatc
     3301 gttgcctgcg gtatgttaac gccttttgcg atcgtaccac tggttatttt ttggcttatc
     3361 gtcaataaaa agctacgcgc caaatacaaa aaactcagta aagatagagc ttcaagaaaa
     3421 ggttcttctt catcctcttc cggtggcggc tcaagtggcg gaggctcagg cggcggattt
     3481 agcggcgggg gcggttcttc tggcggtggc ggcgcgtccg gtcgctggta actcaaccat
     3541 aaaaaacgcc agcccgaagg ctggcatttt taaatcagat aaagtcagac ttatgcctgg
     3601 cctttgatct ctttacgacc gttgtacggt gctttttcgc ccagagcttc ttcgatacga
     3661 atcagctggt tgtatttagc aacgcggtca gaacggctca tagaaccagt tttgatctgg
     3721 cctgcagcag taccaacagc caggtcagcg atggtagcgt cttcagtttc gccagaacgg
     3781 tgagagataa ctgcagtgta gccagcgtct ttcgccatct tgattgcagc cagagtttcg
     3841 gtcagagaac cgatctggtt gaatttgatc aggatggagt tagcgatacc tttttcgata
     3901 ccttctttca ggatcttggt gttggttacg aacaggtcgt caccaaccag ctggattttg
     3961 tcgcccagaa ctttggtctg gtatgcgaaa ccatcccagt cagattcgtc cagaccgtct
     4021 tcgatggaaa cgatcgggta ctgtttggtc agttcttcca ggaagtgagt gaattcttca
     4081 gaggtgaatg ctttgttgcc ttcgccagcc agaacgtatt taccgtcttt gtagaattca
     4141 gaagctgcgc agtccatcgc cagagtgatg tctttgccca gttcataacc agcagcttta
     4201 acagcttcag cgataacagc cagagcttca gcgttggaac ccaggttcgg cgcatagcca
     4261 ccttcgtcac caacagcagt gttcatgcct ttcgctttca gaacttttgc caggtgatgg
     4321 aaaacttcag aacccatgcg gatggcttct ttcacagttt tcgcgccaac cggctgaatc
     4381 atgaattcct ggatatcaac gttgttgtca gcgtgctcac caccgttgat gatgttcatc
     4441 atcggaaccg gcatggagta tttgcccgga gtgccgttca gttcagcgat gtgctcgtac
     4501 agcggcatac ctttagcagc tgcagcagct ttggcgttag ccagagatac agccaggatt
     4561 gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca
     4621 atgccagcct gatctttagc atctttgcca atcagcgcct gagcgatcgg gccgtttacc
     4681 gcagcaacag ctttggttac gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt
     4741 tccagagctt cacgggaacc agtagaagca cctgacggag cagctgccat accgacgaaa
     4801 ccaccctcca gatgtacttc ggcttcaaca gtcgggttac cacgggagtc gatgatttca
     4861 cgaccgatga tttttacgat tttggacatt aggttttcct caagtcacta gttaaactga
     4921 aactccagac aaacaacgcg tacccagggt acgcgttgcc gttctaactt ttttacttac
     4981 ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga
     5041 ccatcacgtg gagtagaagt aaactccgga tggaactggc aagccacgaa ccacgggtga
     5101 ttcggaactt cgatgatctc gaccaactga tcatccccgg aacggcccgc aacgcgcaga
     5161 cctgcatctt caatctgttt caacagcatg ttgttgactt cgtaacggtg acgatgacgc
     5221 tcaacaattg tcggcgcatt gtacagctgg cgaaccaggc tatcgtcaac caactggcac
     5281 tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct tctcgctacg aacttcaacg
     5341 ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa ccgggtactt acagtctggc
     5401 acaaattccg tagagttggc gttctccatg ttggcaacat ggcgagcgta atcaattaac
     5461 gccacctgca tacccaggca aatgcccaga taaggaatat tgttctcacg cgcaaaacgc
     5521 gcggtcgtaa tcatgccttc tacgccacga tagccgaaac cgccaggtac gaggattgca
     5581 tccagacctt taaggatttc aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg
     5641 atgttgacgc tgacacgatt cttcagccca ccgtgtttca gtgcttcaat cacggatttg
     5701 taagcgtccg gcagttcaat gtacttgccg accataccaa tggtgacttc acttaccgga
     5761 ttcgcttctt cgaagataac ctgttcccat tcggacagat ttgcttccgg gcagtttaag
     5821 ctgaatcgtt tacaaatata atcgtccagc ccctgagatt tcaacaggcc cggaatttta
     5881 tagatggaat cgacgtcttt cagagaaata accgcttttt ccggaacatt acagaacaat
     5941 gcaatcttcg cacgttcgtt cgccggaacg gcgcgatctg aacgacaaat caggatgtca
     6001 ggctggatac cgatggagag cagctctttt acagagtgct gagtcggttt ggttttgact
     6061 tcaccagacg ctgccatgta cggcaccagc gtcaggtgca taaacagagt gtgctcacgg
     6121 ccaatttcaa cagccatctg gcgaatcgct tcgaggaacg gcagggattc gatatcacct
     6181 actgtaccgc cgatttcgac cagtactacg tcatgacctt cgccaccttc cagcacgcgc
     6241 tctttgattg cgttagtgat gtgcggaata acctgtacgg ttgcgccgag gtagtcaccg
     6301 cggcgttctt tacgcagaac gtcagagtag atacgacccg tggtgaagtt gttgcggcgg
     6361 ctcattttgg tacggatgaa acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg
     6421 tcttcagtaa cgaacacttc cccgtgttgg attgggctca tagtacctgg atcgacgttg
     6481 atgtacggat ccagtttcat gatggtcaca ttgaggccac gggcttcaag aatggctgcg
     6541 agggaggctg cggcaatgcc tttacccaga gaggatacga ccccgccggt cacaaaaata
     6601 tagttcgttg tcatgctgaa cctgagaagt taggttgaaa agacgatgga ataaccagga
     6661 cgggaaagca gtatacccga acatgacctg tgccacaaac tttcattatc cctcctcttc
     6721 gccagcgcac tattgaaatc aggagtgaga aaatagcccc tttggggtaa atgtttttga
     6781 cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc
     6841 cttagagatc aatttcctgc cgttttacct gttgccagac ttcttccatt gtttcgaggt
     6901 caacacctgt catttccagt ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac
     6961 gctcgaattt ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct aaatgacgag
     7021 ccagattgac cgtggcaaac agcaggtccc ccatttcctc ctccagttta gcctggtcga
     7081 caacagcctg ccgcgcttcg tacatcacct cgtcgatctc ttcgtagact ttatcgacta
     7141 ccggaccaag cgtcgtccaa tcgaagccaa cgttggcgca acgtttctgg attttttgcg
     7201 cacgcattaa agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct
     7261 gcgcgcgctc ttcggttttg atttgctccc aacgggcaag cacttcacta ctgttttcgg
     7321 cagaactgtc agcaaaaaca tgcggatggc gacgctctaa tttatcgcta atcgcagcgc
     7381 aaatatcatt aaagtcaaag cgcccttctt cctgagccat ttgggcgtaa aacaccacct
     7441 ggaacagcag atcgccgagt tcaccgcgca gatcgtcaaa atcttcacgg gcgatggcgt
     7501 ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt
     7561 tatcccacgg gcagccgttt tccgggtcgc gcaggcgctg cataatagtg agcaaacggt
     7621 cgatttgatt cattagaatg tcctgaaaat tgcgggtccg tcaggtggaa acctgtgacc
     7681 agaatagaag tgagttagta acactaccca atcagtacgt taattttggc tttaatgagt
     7741 tgtaattcct ctggggcaac cgttcctttc ttcgttgctc ctcttgcccg ccaggcgata
     7801 ctttttacct gatcagctaa cgctacgcca tcacgttcct gaccggataa aacaacttcg
     7861 aacggatatc cttttgattg cgttgtacaa ggaacacaca gacacatacc tgttttgttg
     7921 ttgtacatga acggactcag gacaacagcc ggacgatgtc cggcttgctc gctacctttt
     7981 gtcgggtcaa aatcaaccca aatcagatcg cccatatcgg gtacgtatcg gcttaccatt
     8041 accagacttc cttatctttc ggctctcccc agtcgatatt ctcgtggagg ttttccggcg
     8101 tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc tttacgcact ggctcaataa
     8161 ttaatttgcc atccaccagg tcaatcttca cttcatcatc aatattcaga ttgagcgcct
     8221 gcattaacgt agccgggatc cgcaccgccg gtgaatttcc ccaacgcttt acgctactgt
     8281 ggatcataac cctttcctca aaccactatc atatgtagat acagtatata tcaatctaca
     8341 ttgtagatac gagcaaattt cggcctaact cccgtgcaac cgacgcgcgt cgataacatc
     8401 cggcacctgg ttgagtttac ccagcacgcg ccccagcact tgcaggttgt aaatctcaat
     8461 ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta cggctggcaa cgccaagcac
     8521 gttcaccttc tcgttggcga gaatggtcgt gatatcacgt aacaacccac tacgatcatt
     8581 agccaccacg cggaccacca gcgaatatcc ggcggagtag ctctcacccc ataccgcgtc
     8641 aacaatgcgt tctggcgcat gggagcgcag ttccgccagt tgttcgcaat cggcgcggtg
     8701 tactgaaata ccgcgtccct gggtaatgaa gccgacaatc tcatctccag gaatcggctg
     8761 gcagcagcgc gcgatgtggt gcatcaggtt accaacacct tcaaccacta cacgaccgtt
     8821 atctttactg cggttttgcg gcgtgtagct tttttgctga agctgtttca gcgcggcggc
     8881 gtcctgctct tcggcactcg gcttattaaa ttgcgattgc aggaagttca ccatctgatt
     8941 gagacggata tccccgccac caatcgccgc cagcaactcg tcgacatcat tgaagttgta
     9001 acgcggcagc agatgttttt ctgcttcttt caggctgatc cccagatgtt ccagctcgtc
     9061 gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg tcctgtttac ggaaccaggc
     9121 gtgaattttc gaacgcccac ggctggttgt gacgtaaccg aggtttgggt ttaaccagtc
     9181 acggctgggg ttcggctgtt tctgggtgat aatttcaatc tggtcgccca tttgcagctg
     9241 gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg atacagcggt gtccgacatc
     9301 actgtggatg tggtaagcga agtccagcgg cgttgatccc gcaggcaaat caacgacatc
     9361 acctttaggc gtaaagacgt acacccggtc gtcaaagacc tggctgcgta cttcgtcgag
     9421 catttcgccg gaatcagcca tctcttcctg ccacgcaatc agtttacgca gccaggcaat
     9481 ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg ccctctttat atttccagtg
     9541 cgcagcaaca cccaactctg catcttcatg catctgtttg gtgcggattt ggatctcaac
     9601 ggtttttcca cccggcccca gaaccacggt atgaatagac tgataaccgt ttggtttcgg
     9661 gttagcgacg taatcgtcaa actcatccgg caggtggcga tagtgagtgt gcactatccc
     9721 cagtgcggca taacaatcct gtaaacgctc ggcgacaata cgtaccgcac gcacatcaaa
     9781 cagctcatcg aaggcgaggt tctttttctg cattttgcgc cagatgctgt agatgtgttt
     9841 cggacgacca tacacttcag ctttaacgcc ttcagctttc atctcagcgc gcagatgacc
     9901 gacaaactct tcgatatagt gttcgcggtc gagacggcgt tcatgcaaca gttttgcgat
     9961 gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa tcttccagct cccatttcag
    10021 ttgcccaatc ccaagacggt ttgccaacgg cgcgtagata ttggtgcact cttttgccgc
    10081 cagtacgcgt tcatcttccg gcgcatcttt tacttcacgc agatgagcaa tacgctccgc
    10141 cagtttgatg accacgcagc gaaaatcatc gaccatcgcc aataacatcc ggcgaacgtt
    10201 atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct ttcagctggc ggatcgccgc
    10261 catatcacgc acgccgtgaa taaggttaac gaccgactta ccgacgctct cacgcagcac
    10321 atcttcgctg actacgttgg catcagccag agggaacagc agcgccgccc gcagcgtgtc
    10381 aatgtccata cttaatgtcg agaggatctc caccatctca acaccacgcc acaataacag
    10441 actggcatcc ggatgcccct gcgtctgttg cagacaatac gcccaggttt cggctaagca
    10501 ctcacacgac ttctggctgg taatacccag acttgcgatc catttttccg gatcaaattc
    10561 accagcctta ttgatatgtg cacttcttac cgcaaccatc gtcctctcct ttagggacca
    10621 gacctgccga aatcggcaaa tcgcaactat ttaacgcgcg agaaaagtac catcgattcc
    10681 agatgtcccg tgtgtgggaa catatccagc atcgccagtc gcgcaatggt atatcctgct
    10741 tttaataacg cttcgctatc ccgagccagc gttgcagggt tacaggatac ataaactata
    10801 cgaataggtt ccagttttat aatttgctgc ataacacctg cggcacctgc tcgcgccggg
    10861 tccagcaaca ctttatcgaa gccgtttttc gcccacggct gctttgtgac atcttcttca
    10921 agattttcgt gataaaacgt cacattctgt aagcagttaa gacgcgcatt ctgctggcct
    10981 ttttccacca gcgccggaac accttctaca cccaccacac tggcagcttg tgtcgccaat
    11041 ggtagtgtaa agttgcccat accgcagaac agatccagta cgcgatcttc aggttccacc
    11101 tccagccatt ccaacgcacg cgctaccatt ttttggttca cacccgcatt aacctgaata
    11161 aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt cataccaggg catctcacca
    11221 gagacggttt cgagtatctc actatcgggg gcgagataca gatccaggcc ttcagaatgc
    11281 gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg gtgcggtatg gcgcaaaatc
    11341 atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac caagatggcg catagcttgt
    11401 aagctgccca ggcatgccct gactttgggc agcaatgctt caagttgggg cactaaaatg
    11461 ggacattgtt taacgtcgac aatgtcactg gagcccgctt tgcgaaaccc catctgaagt
    11521 tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc gacggcgata gccccaggga
    11581 acatcggcga tcacttcaga gacttcgtgt ttcattaatc gggcgagtgc cgcacttttg
    11641 cttcgctgct gtaaatccac gctggcgtgt tgttgctgac agccgccgca tacgccaaaa
    11701 tgaggacagc gtggcgtttc gcgttccggg ctatcgctta accggcgtac gactttagcg
    11761 cgggcatact gttttttatc ctcagtaaca gtaacttccg cgttttcctg cggcaataat
    11821 ccggggataa atagcgtttt gccgttatgt cgcgccacgc cctgaccaaa agagtcgagg
    11881 tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc gtcgttttgc agagtagaat
    11941 tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc ttaattgtcc cataacggaa
    12001 ctccatgacc aactacagcc tgcgcgcacg catgatgatt ctgatcctgg caccgaccgt
    12061 ccttattggt ttattgctga gtatcttttt cgtcgtgcat cgctataacg acttgcagcg
    12121 tcaactggaa gatgccggtg ccagcattat tgagccgctt gcagtttcta ctgaatatgg
    12181 catgagcctg caaaatcgcg aatctatcgg tcagttaata agcgtactgc atcgtcgcca
    12241 ttccgatatt gttcgcgcga tttcggttta tgatgaaaat aaccgactct ttgtcacctc
    12301 caattttcat cttgatccct catcaatgca gctcggcagc aacgtgccgt ttcctcgcca
    12361 gctcactgtc actcgtgacg gcgatattat gatcctccgc acgccgatta tttctgagag
    12421 ttactccccc gacgaatcgc ccagtagcga tgccaaaaat agtcaaaata tgttgggata
    12481 tattgcgctt gagctggatc ttaaatcggt tcgcttgcag caatataaag agatctttat
    12541 ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt atttttggct ggcgcttaat
    12601 gcgcgatgta accggtccga ttcgcaacat ggtgaatact gtcgaccgca tccgtcgcgg
    12661 gcaactcgac agccgagtgg aaggatttat gctcggcgag ctggatatgc tgaaaaacgg
    12721 tatcaactcg atggcaatgt cgctggctgc ttatcacgaa gagatgcagc acaatatcga
    12781 ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg gaaattcaga acgttgagtt
    12841 ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt aaatccgagt ttctggcaaa
    12901 tatgtcacac gagctgcgta caccactgaa tggtgttatt ggttttaccc gcctgacgct
    12961 gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat acgattgaac gttcggcaaa
    13021 taatttgctg gcaattatta atgatgttct cgacttctcg aaactggaag caggtaagct
    13081 gattctggaa agtattccat tcccactacg cagcacgctg gatgaagtcg ttactctgct
    13141 ggcacattct tctcacgata aagggttgga actgacgctc aatattaaaa gcgacgtgcc
    13201 tgataacgtg atcggcgacc cactgcgatt acagcaaatc atcactaacc tggtggggaa
    13261 tgcaattaaa ttcaccgaga atggcaacat tgatattctg gtagaaaaac gtgcgctgag
    13321 taataccaaa gtgcagattg aagtgcagat tcgggatacc ggcattggta ttcctgaacg
    13381 cgatcaatcg cgcttattcc aggccttccg acaggctgat gccagtattt cccgccgtca
    13441 tggtggcacc gggctggggc tggtgattac acaaaaactg gttaatgaaa tgggcggcga
    13501 tatttcgttc catagccagc cgaatcgcgg ttcaactttc tggttccaca ttaatctcga
    13561 tctgaacccg aacattatta ttgaagggcc atccacccag tgcctcgccg gtaaacgcct
    13621 ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg ctggatattt taagtgaaac
    13681 gccgctggaa gtggtttata gcccaacgtt ctccgcgctg cctcccgcgc attacgacat
    13741 gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta acaatgcaac atgagcgatt
    13801 agcgaaagcg gtatcgatga ccgatttcct gatgctggca cttccttgcc atgcacaagt
    13861 caatgctgaa aaactcaagc aagatggtat cggcgcgtgt ctgctgaaac cattaacacc
    13921 tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac aaacaaaaca cgcttttgcc
    13981 tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg gttgatgaca accccgctaa
    14041 cctgaaactt atcggcgcat tgctggaaga tatggtgcaa catgtggaac tttgcgatag
    14101 cgggcatcag gcggttgaac gggcgaaaca gatgccgttc gatttgatct taatggatat
    14161 tcaaatgcct gacatggatg gcattcgggc ctgcgagctc atccaccagc tcccccatca
    14221 gcaacaaacg ccggttatcg cggtaacggc gcatgcaatg gccgggcaaa aagagaagct
    14281 gcttggcgca gggatgagcg attatctggc gaaaccgatt gaagaagagc gattgcataa
    14341 tttgttgttg cgctacaagc ctggcagcgg tatttcgtct cgcgtcgtga cgcccgaagt
    14401 caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg caactggcac tacgccaggc
    14461 agcaggaaaa accgatttag cgcgcgatat gctgcaaatg ttactcgatt tcctgcctga
    14521 agttcgcaac aaagttgagg aacagctggt tggagaaaac ccggaaggcc tggttgattt
    14581 gattcataaa ctgcatggca gttgcggcta tagcggtgtg ccgcgtatga agaatctctg
    14641 ccaacttatc gaacaacagc tacgtagcgg tactaaagaa gaagatttgg aaccggagct
    14701 gctggaactg ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta
    14761 acggacgtct tgtcgagtag caatcggctt tttatgacgc tgttttcaga ttggtgacaa
    14821 agtgcctgtg tttatgccgg atgcggcgtt aacgccttat ccggcctaca aaaccaatta
    14881 aattcaataa attaaaaagc atgtaggcct gataagcgta gcgcatcagg caattttgtg
    14941 tttggcatca gtctcaaacc ggctccagat agagccggtt ttggtttttt gtcttaacgc
    15001 accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa tcagatactg cattcccatc
    15061 gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct catgggcttt catcacttga
    15121 tccatatcga tttctacacc cagccccggt ttttctggca cctgtaccag cccgcctttg
    15181 atctcaaacg gttctttggt caggcgctga ttaccttcct gccagatcca gtgcgtatca
    15241 atagcagtaa ttttacccgg tgcagcggcg gcaacatggg taaacatcgc cagggaaata
    15301 tcgaagtggt tgttagagtg tgaaccccag gtcaggccaa attcatggca catttgcgcc
    15361 acacgtaccg aaccttgcat tgtccagaaa tgcggatccg ccagcgggat atcaacggat
    15421 tgtaacgaga gcgtatggcc catctgccgc cagtcggtgg cgatcatatt ggttgcagtc
    15481 ggcaggccag tcgcgcgacg gaactctgcc atcacttcac gcccggagaa accttgctcc
    15541 gcaccacacg gatcttctgc ataagccagc gaacctttca ggtatttgcc gattttaatc
    15601 gcttcgttca gcgaccaggc accgttagga tcgagcgtaa tacgcgcctg cgggaagcgt
    15661 tgcgccagtg ccacaataga ctcggcctct tcttccccgg ccagtacacc gcctttcagt
    15721 ttgaaatcgt tgaagccata tttttcatac gccgcttccg ccaggcgcac caccgcatcc
    15781 ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc atgagtcatc cggctggctt
    15841 tgatacggca gaggtgtggc tttgcgatta ccgacgaaga acagataacc gagcatttcg
    15901 acttcgctac gctgttgacc atcgcccagc agggatgcca cgttaacccc cagatgctgc
    15961 cccagcagat ccagcattgc cgcttctatc ccggtaacta catgaatagt ggtacgcaaa
    16021 tcaaatgtct gcaaaccgcg cccgccggca tcacgatcgg caaaagtatt acgcaccagc
    16081 gtcagaacgt ttttgtattc acccagcgtt ttacccacta ccagcggaat tgcatcttcc
    16141 agggttttac ggattttctc gccgccggga atttccccta cgccagtgtg accagaatta
    16201 tctttgataa tcacaatatt acgcgtgaag aacggtgcgt gtgcaccact cagattcatc
    16261 agcatactgt catggcccgc caccgggatg acctgcattt cagtaacaac aggcgtcgta
    16321 aattgagaac tcatatttat gtcctttttc agaatcaatg acggccgaaa acgggacgtt
    16381 tacggtcaaa ggtccagccg gggatcaggt actgcatcgg gcctgcgtcg ttacgcgcac
    16441 cgccaggcag acgtttatag gcctcatgtg ccttttgtac ctgttcccag tccagttcca
    16501 cgcccagacc gggcgcatca ggaacggcaa tttttccgtt tttaatctcc agcggatttt
    16561 gggtcaggcg acaatcgccc tcctgccaaa tccagtgggt atcgatagcg gtaggattac
    16621 ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga gatatcgaaa tggttgttgg
    16681 aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg cgccactcgg actgcaccgg
    16741 aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac cgcattgagc atcaccgcat
    16801 gacccatttc gcgccagttg gtggcgatca tattagtcgc gacgggcaag ccggtcgccc
    16861 gccgaaactc cgccatcact tcacgtccgg agaagccctg ttctgcgccg catggatctt
    16921 cggcataggt aagaacatca ttcagccctt tgcacagaga aatggcttca tcaagcagcc
    16981 atgcaccgtt gggatcaacg gtaatccgcg catccgggaa gcgtttcttc aatgcacgaa
    17041 cagtgtcgat ttcttgctcg ccaggtaaca cgccgccctt aagtttgaaa tctttaaagc
    17101 cgtagcgatc ctgtgaggct tccgccagac gcacaacggc ttcgctgttc atcgctttct
    17161 gatggcgcaa ctgataccac tcatggttgc ccggcgtatt ttccagataa ggaagatcgg
    17221 ttttggtccg atcaccgata taaaacagat aaccgaggac ggtaatagca tcgcgttgct
    17281 tgcctggccc taacagttcg cagaccggaa cattcagcgc cttacctagc aggtcaagca
    17341 aggcggcttc cagcgccgcc acggcgttaa cgcgcaattc aaaggtccag gcccctttac
    17401 cgaaggtatc aaaatcggct gcttgattac ctttatgcac ctgctgaacc actttattca
    17461 ggcgcgcaac ttcctagccc agcaccatcg gaatagcatc gaccaatgtc tgataaatca
    17521 cctctccgcc tggcgcttca ccaatgccgg tatgcccggc gttatcggtg agtaccacaa
    17581 tattgcgagt gaaatatgcg ttatgcgccc caccaatatt aagcaacatg ctgtcgtacc
    17641 cggcgaccgg aatgactttc atatcagtaa taacaggact ggattgtgtc gtcatcttat
    17701 tgccccacga caggtttcag ctcgatacgc ttgatatcgc ccaccagcac caggtagctc
    17761 agtaccgcga ttaaggcatg aacaccaaca taaatcagcg ccccattaaa cgagccagtc
    17821 gtgccaacga tataaccaat tgcgattggc gtgacgatgc cagaaatgtt accgaacatg
    17881 ttgaacaggc caccggaaag accgctgatc tcttttggcg cggtatctgc cattactgcc
    17941 caacccagcg ccccaatgcc cttaccgaag aaggccagcg ccataaagcc gatgatcatc
    18001 cactcaacgt tgacgtagtt gcagaacacc atcaccatcg acagcaacat gcccattacg
    18061 atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc gcatcagcca gtcggaaata
    18121 atcccaccca gcacaccacc gataaaacca caaaccgccg gaacggaagc cacaaagccc
    18181 gctttcagaa tcgacatccc acgcgcctgc accagataaa ccggaaacca ggtaatgaaa
    18241 aagtaagtca gggcgttgat acagtactga ccgatataaa cgccgatcat catccgtgac
    18301 ccgagcagtt gtttgatctg cccccacttc acgctgaacg gcactttaac tttggtgttt
    18361 tgctgatcca tattgatcag cgcgccaccc gcggcgatgt actccagctc tttctgattt
    18421 acccccggat gttgatttgg ctcgtggatg actttcaacc agataaagct gatggcaatg
    18481 cccagaccgc ccataaagaa gaagacgtgt gaccagccca cttcatgcgt cagccagccc
    18541 ataatcggcg cgaagatcac cgttgcgaag tattgagcgg agttaaaaat cgacaccgcc
    18601 gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc gactgttgcc ggggaaagat
    18661 ggcgcttcag caagcccgac caggaagcgc agagtaaaca gggcaacgat aatgccgaat
    18721 ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat aaagatcgac
    18781 cagaagtaga cgcgttttga accaaaacga tccagcaacc aaccaccagg gatctgcccg
    18841 ataacataag cccatgagaa ggcagagaac acatagccca ttcccacggg atcaaggccg
    18901 atatctttgg ccatttccga accggcaata gaaagcgtag cgcggtcgcc gtagttgaag
    18961 gatgtgacga taaacaacat caccactatc cagtaacgag cattcgtgcg tttttccaca
    19021 ctgctcgcag cctgacttaa agaactcatt gttgcactcc tgaaaattcg cgttagccac
    19081 gctcactctg gactgcgaca tcgccaggaa atcagaggtg acgtagggtg ttttttgccg
    19141 tttttatagg tcgttcgccg aatacggcgc gtgtttatat ctggcaatag cagtataaaa
    19201 agcgcgccat agcggctcac cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga
    19261 ttttggcatt agccctggac ggtggaatcc acttcacgga aatgaaaaca agaacaagaa
    19321 aggaagggtt aaaacgaaga aataaagaag agtatgaaat ggatcgcttg actccaggca
    19381 aacgccagta aaaatccgcg ctatgaagca gtttttactg gcatttgcct gaaaagattc
    19441 gattcagcac cgctaaaacg acatttaccg ctcgctgaac atatcaggac aacagcgtgc
    19501 cccactgttc gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa
    19561 tcaacagcat ttcgccaacc cgctgagcgc tctgttcctg cagcaaggca tcgaactgtt
    19621 tgccgccatt gcagaaattc acataactac tgtcgccgag cgcaatcacg ccataacgca
    19681 gattcggctg gaagcccaga ctatctttga ttccctgaaa gagcggcaca atgctatcag
    19741 gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgatagggca
    19801 gccagtcgct taattcagga tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa
    19861 ttgcttctgc ttcttccgcc actaacagcg aatttccgta catggtgccg acaaaaatac
    19921 caatttccgc cataccgttt tctccctgga ttaggaactt ctctctccat cctgacccga
    19981 tggcgcactg aactcaaccc tttcattttc aggaagcaga ccgcgccagc caaattgtga
    20041 taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct caccagtaaa
    20101 aggatgcgtc aatgacaact gactggcatg cagcattaac cgctgaagac caaaatgctc
    20161 agcaccgctg cgattctggc gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg
    20221 caaatgggca agatgtcggc gaagctgatg tttgcgtccg gttttcggct ccagttccac
    20281 caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag
    20341 accgcgataa tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc
    20401 gattttgtcc agttcttcca ccagtggata atccagcacc gcttcttcca tcaaccagcc
    20461 gcgcacaatc gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag
    20521 ccgtccggcc tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg
    20581 atgagcagta aaaacatgct ggcctatctg gtcacgcacg gtttgcatga ccactacttt
    20641 ctcgtcgcga tccagccagc tacggtgaac cagccagccg gagggtttat ttaccgcaac
    20701 cagccattca tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca
    20761 tccagttttt ccaactcagc cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc
    20821 atttcataat agggtgcaac ggcaaaagcg cccggtaacg gctggttgtt atttaacaaa
    20881 tcgtgcattc gcgggatcag cacccactgc aaccactcca gtggttccat ggtgtccata
    20941 aagaacggtt gggtactatt aaattgatgc ggctggggtt catcgtttcg ccagtgctga
    21001 tgttcacgca gtaacgcttc aagcgcctgc aactggagac gaacgcggtc atgagtggtc
    21061 ataaagcaac ctcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc
    21121 actgtattca cagtgctccc ggttcgtttc gcagcattcc agctactttt gttgctccct
    21181 gctcatcctt gacaactttt cctctggcca tgcggccaat cgttcatcct gaactattgc
    21241 ttcctgctca caccaccccg atgtgatact tcatcctgaa gtgtcccagg ccttcctgac
    21301 ccaccgaatc atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc
    21361 tgcgtcatcc tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc
    21421 ttcagaagtg tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata
    21481 gaatcgtctt tttcgcgaac tcttacaagg cgcttacaga caatgcctta aggaaaattt
    21541 catatgaaag ttaggaatta acaagcaaca tgttgttttt aaatgaaaat aacgattctt
    21601 atctttttaa gtattcgctt atttcctggc gatctcctac agagtctgta agagatctct
    21661 cacaaccaga tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca
    21721 agatttgggg ctaaatgcgt ccgtttacgt gttcccagcg tctctttaca cacttcaccg
    21781 gagagattac acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg
    21841 ggtggcagtt ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga
    21901 cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg
    21961 tgcatatccc cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg tatcacaata
    22021 tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag
    22081 tatacggcat cttcagtagt tgaaataatg cacggtgaag gaacgccgta cagttcctca
    22141 cttaacggcc agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct
    22201 ttcagggctt gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac
    22261 ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag
    22321 gcacttgcgg gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc
    22381 ctactgcaag gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac
    22441 ctgccttttc aaggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa
    22501 ggtttgccgc aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt aaatctgatt
    22561 gagtcgaaga gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg
    22621 gatgaggtgc gccagacgct ggagcgcgac ttaagcactt gcgctcaggg tgaggttagc
    22681 gtggcgttat atcgtcttga tgaactggaa ggccagccga taggtcattt caatggcact
    22741 tgcattgatg accaggatat cactatcgat aactatgaat tcactactga ctatctggag
    22801 aatgccacca gtggtgaaaa agtagtggaa gagacgctcg tcagccacct gctgaaatca
    22861 aactgcctga tcacccatca accagattgg ggttcgatcc aaattcagta tcgtggacgc
    22921 caaattgaca gagaaaaact gctgcgttac ctggtctcat tccgtcatca caacgagttc
    22981 cacgaacagt gcgtggaacg catctttaat gacctgttac gcttctgcca gccagagaaa
    23041 ttgagcgttt acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt
    23101 aatagcgatt ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt
    23161 tgcgtgctgg attcacgcag aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa
    23221 agggaatgac gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt
    23281 ggctccatgg atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc
    23341 gacctctatc aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc
    23401 gataacagca aagaattgct gtctcgtttt gaaaatttcg atattaacgt cttgcgccgt
    23461 gaacgcggcg taaagctgga actgattaat cccccggaag aggcttttgt cgatgggcga
    23521 attattcgcg ctttgcaggc caacttgttc gcggttctgc gagacattct cttcgtttac
    23581 gggcaaatcc ataataccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc
    23641 actaacctgg tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg tgaagcgcca
    23701 aatatggtgg tctgctgggg cggtcactca attaacgaaa atgagtattt gtatgcccgt
    23761 cgcgtcggaa accagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccggga
    23821 gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg ttacaaagac
    23881 agtcgtttta ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccgctg
    23941 gtcaacgaat tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc
    24001 gctcacggca tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg
    24061 ctgggaattt taatgaaccc ggccaacaaa gatcaggttt taccattgat cctcaccggc
    24121 ccgaaagaga gcgccgacta cttccgcgta ctggacgagt ttgtcgtaca tacgctgggc
    24181 gaaaacgcgc gccgccatta ccgcataatc attgatgacg ccgctgaagt cgcccgtcag
    24241 atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgtg atacaggcga tgcctacagc
    24301 tttaactggt caatgcgcat tgcgccagat ttgcaaatgc catttgagcc gtctcacgag
    24361 aatatggcta atctgaagct ttacccggat caacctgttg aagtgctggc tgccgatctg
    24421 cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt
    24481 gaagagtttg gtccttacaa aatcaacggc gataaagaga ttatgcgtcg tatggacgac
    24541 ctgctacagg gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct
    24601 tgctacgaaa tctgcacgta atctcccctc ccccggtgac gtttgttgcc gggttttctc
    24661 gtttttggtc acttactcat caactcattt catttgttat atgaatgttt cttac
//
LOCUS       DAZFKY010000059        24289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFKY010000059 DAZFKY010000000
VERSION     DAZFKY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(51..932)
                     /gene="prmA"
                     /locus_tag="ACHKCL_002086"
     CDS             complement(51..932)
                     /gene="prmA"
                     /locus_tag="ACHKCL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5763053.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(944..2395)
                     /gene="panF"
                     /locus_tag="ACHKCL_002087"
     CDS             complement(944..2395)
                     /gene="panF"
                     /locus_tag="ACHKCL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5763054.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2385..2627)
                     /locus_tag="ACHKCL_002088"
     CDS             complement(2385..2627)
                     /locus_tag="ACHKCL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5763055.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2736..4085)
                     /gene="accC"
                     /locus_tag="ACHKCL_002089"
     CDS             complement(2736..4085)
                     /gene="accC"
                     /locus_tag="ACHKCL_002089"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5763056.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4096..4566)
                     /gene="accB"
                     /locus_tag="ACHKCL_002090"
     CDS             complement(4096..4566)
                     /gene="accB"
                     /locus_tag="ACHKCL_002090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5763057.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5544..6518)
                     /locus_tag="ACHKCL_002091"
     CDS             complement(5544..6518)
                     /locus_tag="ACHKCL_002091"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5763058.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6670..8610
                     /gene="csrD"
                     /locus_tag="ACHKCL_002092"
     CDS             6670..8610
                     /gene="csrD"
                     /locus_tag="ACHKCL_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5763059.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8915..9958
                     /gene="mreB"
                     /locus_tag="ACHKCL_002093"
     CDS             8915..9958
                     /gene="mreB"
                     /locus_tag="ACHKCL_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5763060.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10024..11127
                     /gene="mreC"
                     /locus_tag="ACHKCL_002094"
     CDS             10024..11127
                     /gene="mreC"
                     /locus_tag="ACHKCL_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5763061.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            11127..11615
                     /gene="mreD"
                     /locus_tag="ACHKCL_002095"
     CDS             11127..11615
                     /gene="mreD"
                     /locus_tag="ACHKCL_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5763062.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11624..12217
                     /gene="yhdE"
                     /locus_tag="ACHKCL_002096"
     CDS             11624..12217
                     /gene="yhdE"
                     /locus_tag="ACHKCL_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5763063.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12207..13676
                     /gene="rng"
                     /locus_tag="ACHKCL_002097"
     CDS             12207..13676
                     /gene="rng"
                     /locus_tag="ACHKCL_002097"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5763064.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13744..17544
                     /gene="yhdP"
                     /locus_tag="ACHKCL_002098"
     CDS             13744..17544
                     /gene="yhdP"
                     /locus_tag="ACHKCL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5763065.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17700..19145
                     /gene="tldD"
                     /locus_tag="ACHKCL_002099"
     CDS             17700..19145
                     /gene="tldD"
                     /locus_tag="ACHKCL_002099"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5763066.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19279..20208)
                     /gene="aaeR"
                     /locus_tag="ACHKCL_002100"
     CDS             complement(19279..20208)
                     /gene="aaeR"
                     /locus_tag="ACHKCL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5763067.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20391..20594
                     /gene="aaeX"
                     /locus_tag="ACHKCL_002101"
     CDS             20391..20594
                     /gene="aaeX"
                     /locus_tag="ACHKCL_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5763068.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20602..21531
                     /gene="aaeA"
                     /locus_tag="ACHKCL_002102"
     CDS             20602..21531
                     /gene="aaeA"
                     /locus_tag="ACHKCL_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5763069.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21537..23504
                     /gene="aaeB"
                     /locus_tag="ACHKCL_002103"
     CDS             21537..23504
                     /gene="aaeB"
                     /locus_tag="ACHKCL_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5763070.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23596..23868
                     /locus_tag="ACHKCL_002104"
     CDS             23596..23868
                     /locus_tag="ACHKCL_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5763071.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23924..24187)
                     /gene="yhcN"
                     /locus_tag="ACHKCL_002105"
     CDS             complement(23924..24187)
                     /gene="yhcN"
                     /locus_tag="ACHKCL_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5763072.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5748 a         6135 c         6606 g         5800 t
ORIGIN      
        1 tgtgagcttg ctcgcacttc gccccgcgtc accctatggc gatgcgaagg ttaattctta
       61 cgaccggtaa tacggcacca ctcttctttt tccacgaccg ggtccagagc gaagctgtcg
      121 gcataggctt cgcaaacgct ctccgcctgg cttgccagaa taccagaaag gcccagtaag
      181 ccgcctgaaa ctggcaggac gctaattaac ggtgccagtt cacgtaatgg gcctgcaagg
      241 atgttagcga ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt
      301 tccagacggt cagaaacgcc attacgttcg gcgttatcgc ggctggcctg aatcgcctgc
      361 ggatcgatat caataccaat ggcttttgct gcacccagtt tcagcgccgc gatcgccaga
      421 atgccggaac cacagccaaa gtcgatgact gttttaccgg ttaaatcgag gctgtcgagc
      481 cattgcaggc acagagaggt ggttggatgg gtacccgtac caaacgccag ccctggatct
      541 aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt
      601 cgttcgccaa agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct
      661 tctagttgtt cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc
      721 gccaccacgt cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc
      781 cacaggcgcg tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa
      841 gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa gatcttccgc gttcgcgccg
      901 gtggtgttca gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca
      961 aaacggtagc ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac
     1021 ttagtagtaa cgagggcacg atagggtgga agcccaggta ctgaatattc agcgtcgcga
     1081 gtacggcata cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc
     1141 gttcccagta aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg
     1201 ccagcaaatt cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg
     1261 cgccgagaac taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt
     1321 gatccggacg gatattcaga tagagatctt taatgatcgt agcggaactt tgtagcaatt
     1381 gggcgttaat tgtcgacatg atcgcagcca taggtgcagc cagaaagatc ccggcagcaa
     1441 acggtggcag cacttttacc attaacgtcg ggatcaccag atccggtacg gtgagatcgg
     1501 ggatcactgc acgacctaac gctccggcta ggtgcatacc gaacatcagg attgcgacta
     1561 caatcgtacc gatgatgatc ccccggtgta ctgctttgct gtctttataa gagatacagc
     1621 gcaccgcagt atgcggcagg cctataacgc caaagcacac cagtacccag aacgacgtca
     1681 taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg
     1741 tttgcaaggt ctgtactgcg ttgcttaagc caccagcggc atgtaccacg ccaataagca
     1801 gcacaacggt gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc
     1861 gaaagccacc aaaggcggta tataacgcaa tgctgatacc aaaaatcagc agcccggttt
     1921 cataaggaat acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca
     1981 tcgcaccaac gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct
     2041 ggtagcgggc aaacagcata tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg
     2101 caaacttctt gccgagaata ccgagtgaaa gccagactgc cggaagctga atcatcgcca
     2161 gcaataccca gccaaggccg tatttgtaag cagctccagg cccgccgata aacgaactgg
     2221 cactgatata agtcgcggtg agcgtcatcg ccagcacaat accgcccata gagcggctgc
     2281 cgaggaaata ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga
     2341 taccgaacac caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca
     2401 tcctccagcg ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac
     2461 agcagcggcg tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg
     2521 gcaacgccag gtaagtaagc ggctactaac caaactgcca gatacaaaag ggtcagcccc
     2581 agcgcccagc gcgcctcttt atgggcctga acaaaacgag tgtccatttt ttgtccctga
     2641 tgggtgaaga aagcgaggat tgtaccttat gggtgttgtc gatccccagt aataaaaaag
     2701 gccggaaaat ccggcctttt gacgcttttg cagtcttatt tttcctgaag accgagtttt
     2761 ttctccagat agtggatgtt agtgccacca tgctggaagt tctcgtcatt catgatgcgg
     2821 atctgcagat caacgttggt tttgataccg tcgatgatca gctcctgcag cgcattcttc
     2881 atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa tcagcttacc gatcattgag
     2941 tcatagtacg gcggtacggt gtagcccgcg tagatatgag actcccaacg tacgccaaaa
     3001 ccgccaggtg cgtggaaacg ggtgattttg cccggacttg gcaggaaggt gttcggatct
     3061 tcggcgttga tacgacattc caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc
     3121 gacagcggtt gaccggcagc gatacgcagc tgttctttga tcaggtcaac gccggtgatc
     3181 atttctgtaa ccgggtgttc tacctgaata cgggtgttca tttcgatgaa atagaactcg
     3241 ccgttttcga acaggaactc gaaagtacct gcaccgcgat agccgatatc aacacacgct
     3301 ttagcgcaac gttcgccgat gtagcgacgc agttccgggg taatgcccgg tgctggtgct
     3361 tcttcgacca ctttctggtg gcggcgctgc atggagcagt cacgttccgc cagatagata
     3421 gagttgccct gaccgtcagc cagtacctga atctcgacgt ggcgaggatt ttccaggtat
     3481 ttctccatgt aaaccatatc gttgctgaaa gcagctttcg cttccgcacg ggtcatggag
     3541 atggattgtg ccagttcagc gtcgccgcgc actacgcgca taccgcgacc accgccgccg
     3601 ccggaggctt tgataatcac cggataacca atgcgtttag caatggcacg gtttttatcc
     3661 atatcgtcgc ccagcgggcc gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc
     3721 gccgcgattg cggatacttt gtcgcccatc aggcgaatgg tttctgcttt cgggccaatg
     3781 aagataaagc cggagcgttc aacctgctcg gcaaagttgg cgttctcgga gaggaagccg
     3841 taacccggat ggattgctac tgcgccggtg atttcagcgg cgctgatgat tgccgggatg
     3901 ttcagataac tttttactga cggagcaggg ccaatacaga ccgtttcatc tgccagtaat
     3961 acgtgtttta gatcgcgatc cgcgctggag tgcacagcga cagtcttgat gcccagttct
     4021 ttacaggcac gaagaatacg caatgcaatc tcgccgcggt tggcaataac aattttatcc
     4081 agcatgttcg cctcgttact cgatgacgac cagcggctcg tcaaattcta ccggttgtcc
     4141 actttcgacc agaattgctt tcacggtacc ggatttgtcc gcttcgatct ggttcatcat
     4201 tttcatggct tcaacgatgc acagggtatc gcccacgttg actttctgac ccacttcgat
     4261 gaacgctttt gcatccgggc ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac
     4321 gatgtgacca ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc
     4381 ggctgcgtta gattgagctg gctgctgcat cattggtgca gcgtaagctt gttgcatcac
     4441 agggaaactt gcggcaggag ctgcacggct aatgcgtact gactcttcgc cttcagaaat
     4501 ttccagttcg gagatgcctg attcttcaac cagctcgatc agttttttaa tcttacgaat
     4561 atccatgagt gggttccgta ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg
     4621 tctgtaaagc gtatgccccg acagctgtat gcatagcgat aaattccagc aggtcgggac
     4681 gccggtctat tttgccccag agtgtcaatg ttagacttga cggacattgt gcagcgatct
     4741 gctgctatcc tccggcaaaa aacaaaatat accttcgttg cgccaatgtc accttttccg
     4801 cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt ataacgattt cgtagcaatt
     4861 ggcagctaaa tactggtctt atcagggaag ataatcaaca actaacatgt aaataacctt
     4921 caacaccgtg taatttgcaa caagccgcac aattcacgaa attagcgcca tcatcgacgg
     4981 aacttcttat aacgtaaggt gaaaagctgt aaagccagcc ctgcgaagat gaggggctgc
     5041 aatgagataa tcgtcacaga ccacaaataa tgtagggatg ccaggatcgc gacaagatag
     5101 acgacattac gcagcggttg ctggggatta cccaattttc actgcatcgc ctggatagat
     5161 gtgaatgcta aagcacgcaa aattaaccga ttgacaatgc tcagcattaa ataggttcga
     5221 gtgattaatt tgtagtcaaa caaaatcaga ttattcacct catggtacca gaaataagta
     5281 aggggttaag cgtagcgtcg cccaggcaaa gcaccataaa cctaacaggc gacggaggcg
     5341 tatcaataat agctgtttag tggagtatca atgcgcatca atttatggtc agtcgaaatc
     5401 atgactttcc agtttggatg atgttgtaca acttcatgac caaggtgttc gacgaaagct
     5461 gtccggctaa ccgcttttcc cgttaaccga ttgaataatg atttcaaaat gagttccctc
     5521 tcttattatt ccttgctaaa tggttagtta accttcacca gcgtgcgacc ctggatctgg
     5581 ttattaatga tggcctcggc aaagttcggt gcctctgaca gagatatctc ttttgccgcc
     5641 tgggtataga atgattccgg taaatcggcg accagtcgct gccaggcttg tgcgcggcgt
     5701 tctggtggcg tcattactga atccacccct tgcaaacgga cattacgcag aataaatggc
     5761 atgaccgtgg ttggcagagt aaaaccaccc gccagaccac aggccgccac gcagccgccg
     5821 taattcattt gcgccagcac ttttgccagc actttgtcgc caacagtgtc aattgcccca
     5881 gcccagactt gtttttccag aggacgggat tcggcaaact catcacgagg gagaatacgg
     5941 ctggcaccta aacttttcag atattcatgg gtactttcgc gaccggaaac ggcaacgacc
     6001 tgataaccca acttatgcag cagcgccacg gcggtactgc cgacgccacc actggcaccc
     6061 gtcacgacaa tctccccgtc ctgcgggcga acaccggcat cttccagcgc catcacacac
     6121 agcatggcgg taaaaccggc agtaccgata atcattgctt tacgcgcgtc cagcccttgc
     6181 ggcatggcaa ccagccagtc acctttcact cgcgcctgct ccgccagccc accccagtgg
     6241 ttttcaccaa cgccccagcc agtgagtaac acctcctgac cggcatgaaa acgcggatct
     6301 tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa attacggatg
     6361 atttttccct taccggtaat cgccagcgca tctttatagt tcaggctcga ccagtgaaca
     6421 tcgaccgtga catcgccctc cggcaggcga ctttcgtcca gagtctgtac tgatgcgaga
     6481 gttttgccgt cctgctgttc taaaagtaac gcctgcataa gtggtcctca tgtgcatgat
     6541 ggattggaaa ataatttctg aagactatac tcgctaatgg aaaagcaaag ccgatgaagc
     6601 gcaataaatt gcgagataaa tctgatttgc tagtatgccc gcttcctcac tatcggagtt
     6661 aacacaagga tgagattaac gacgaaattt tcggcctttg ttacgctgct caccgggtta
     6721 acaatttttg tgactttgct gggctgttcg ctaagtttct acaacgccat tcagtataag
     6781 tttagtcatc gcgttcaggc ggtggcgacg gcgattgata cccaccttgt gtcgaatgac
     6841 ttcagcgcat taaggccaca aattaccgaa ttaatgatgt cggcagatat cgttcgtgta
     6901 gacctgctcc atggtgataa gcaggtttat accctggcca gaaatggtag ttatcgtccg
     6961 gttggcacca gcgatctgtt tcgtgaactg agcgttccgt tgataaagca tccggggatg
     7021 tcgttgcgtc tggtttatca ggatccgatg ggcaactatt tccattcgtt gatgaccacc
     7081 gcgccgctca caggggcgat tggctttatc attcttatgc tctttctggc ggtacgctgg
     7141 ttacaacggc aacttgccgg gcaagaattg ctggaaaccc gggctactcg tatcttaaac
     7201 ggtgagcgtg gctctaatgt gttgggaacc atctatgaat ggccgcccag aaccagcagt
     7261 gcgctggata cgctgcttcg tgaaattcag aacgcacgcg aacaacacag ccgtcttgat
     7321 acgctgatcc gctcttatgc cgcacaggac gtgaaaaccg gcctcaataa ccgactcttt
     7381 ttcgataatc agttagcaac gttactggaa gatcaggaga aagtaggtac ccacgggatc
     7441 gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg atacctgggg gcacagccag
     7501 gttgaagaac agttcttcac tctgacgaat ctgctgtcga catttatgat gcgctaccct
     7561 ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg cgctgttacc gcaccggacg
     7621 ttaaaagagg cagagagcat cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac
     7681 aataaaatgc tcgatcgcga cgatatgatc cacattggta tctgcgcctg gcgtagtggt
     7741 caggataccg agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc gggattgcag
     7801 ggcggcaata gctgggctat ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt
     7861 cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa
     7921 ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg catctttgat
     7981 ggtaatgaag aggttagctc ggcggagtat atgccgatgg tcttgcagtt tggcttatcg
     8041 gaagagtatg accgtctgca aatcagccgt cttattccac tattgcgtta ctggccagag
     8101 gaaaatctgg cgattcaggt taccgttgag tcgctgattc gcccgcgttt tcagcgttgg
     8161 ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat tattgaactt
     8221 gcagaggccg atgtaggtca acatatcagt cgtttacaac ctgttattcg tttagtgaat
     8281 gctttagggg tacgggtagc cgtcaaccag gctggtttga cgctggtaag caccagttgg
     8341 atcaaagaac ttaatgttga gttactcaag ctccatccgg ggctggtcag aaacattgag
     8401 aagcgaacgg agaaccagct gctggttcaa agcctggtgg aagcctgctc agggaccagc
     8461 acccaggttt acgccaccgg tgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc
     8521 ggtgttacag gcgggcaagg ggattttttc gcgtcctcac agccacttga tactaacgtg
     8581 aaaaaatatt cacaaagata ctcggtttaa cctgccgttt aatccgtttt cacgtagaat
     8641 aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt gcagcacata
     8701 tgcagatgaa tgaccttacg cggttgcaaa caggcgagga atgctgctga tgcattaagc
     8761 ctttctggac tcaggcagag atttgtaaca aaggaaacga actgcactaa ttttcaccgt
     8821 agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc
     8881 ttttccgccc cagctttcag gattatccct tagtatgttg aaaaaatttc gtggcatgtt
     8941 ttccaatgac ttgtccattg acctgggtac tgcgaatacc ctcatttatg taaaaggaca
     9001 aggcatcgta ttgaatgagc cttccgtggt ggccattcgt caggatcgtg ccggttcacc
     9061 gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa
     9121 tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa
     9181 aatgctccag cacttcatca aacaagtgca cagcaacagc tttatgcgtc caagcccgcg
     9241 cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc
     9301 cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat
     9361 tggtgctggc ctgccggttt ctgaagcgac cggttctatg gtggttgata tcggtggtgg
     9421 taccactgaa gttgctgtta tctccttgaa cggcgtggtt tactcctctt ccgtacgcat
     9481 tggtggtgac cgtttcgacg aagctatcat caactatgtg cgtcgtaatt acggttctct
     9541 gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt atccgggcga
     9601 tgaagtccgt gaaatcgaag ttcgtggccg taacctggca gaaggtgttc cacgcggttt
     9661 taccctgaac tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag
     9721 cgcggtaatg gttgcactgg aacagtgccc gccggaactg gcttccgaca tctccgagcg
     9781 cggcatggta ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga
     9841 agaaaccggc attccagtcg ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg
     9901 cggcaaagcg ctggaaatga tcgacatgca cggcggcgac ctgttcagcg aagagtaatc
     9961 ggatgcaggc aggggaagcg tctgtttacc ctgcctggtc tgatacgaga atacgcataa
    10021 cttatgaagc caatttttag ccgtggcccg tcgctacaga ttcgccttat tctggcggtg
    10081 ctggtggcgc tcggcattat tattgccgac agccgcctgg ggacgttcag tcaaatccgt
    10141 acttatatgg ataccgccgt cagtcctttc tactttgttt ccaatgctcc tcgtgaattg
    10201 ctggatggcg tatcgcagac gctggcctcg cgtgaccaac tagaacttga aaatcgggcg
    10261 ttacgtcagg aactgttgct gaaaaacagt gaactgctga tgcttggaca atacaaacag
    10321 gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc gtcaggatga gcagaaaatg
    10381 gtgactcagg ttatctccac ggttaacgat ccttatagcg atcaagttgt tatcgataaa
    10441 ggtagcgtta atggcgttta tgaaggccag ccggtcatca gcgacaaagg tgttgttggt
    10501 caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga tgcgacccac
    10561 gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt
    10621 acggatgatt tgcagcttga gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg
    10681 ctggtgacct ccggtctggg cggtcgtttc ccggaaggct atccggtcgc ggttgtctct
    10741 tccgtaaaac tcgataccca gcgcgcttat actgtgattc aggcgcgtcc gactgcaggg
    10801 ctgcaacgtt tgcgttatct gctgctgctg tggggggcag atcgtaacgg cgctaacccg
    10861 atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag
    10921 gtattgcctt cgccagacgc gatggggcca aagttacctg aaccggcaac ggggatcgct
    10981 cagccgactc cgcagcaacc cacgacagga aatgcagcta ctgcgcctgc tgcgcccaca
    11041 cagcccgctg ctaatcgctc tctacaaagg gctacgccgc cgcaaagtgg tgctcaaccg
    11101 cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg tagccaggga cgctgggtaa
    11161 tctggctctc tttcctcatt gcgcttttgc tgcaaatcat gccctggccg gataacctga
    11221 ttgttttccg gccaaactgg gtgttactca tcttgttgta ttggatcctg gccttgcccc
    11281 atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat actggatctg atcagcggct
    11341 cgacgcttgg cgtacgcgta ttggcgatga gcatcattgc ttacctggtg gcgctgaaat
    11401 accagctttt ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc
    11461 tggtggtgga tattattgtt ttctgggcag agtttttagt gattaacgtc tctttcagac
    11521 cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg gccgtggatt ttcttgctga
    11581 tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt tctatgactt ctctgtattt
    11641 agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa cttggcgtga cctttgaacg
    11701 tattgttacg ggcattgagg agcagcgtca gccgcaggag agcgcgcagc agtatgttgt
    11761 gcgtctggcg cgcgagaaag cacgggcagg tgtcgcgcaa acggcgaagg atctcccggt
    11821 gctgggtgcg gatactatcg ttatcctgaa cggagaagtg ctggagaaac cgcgcgacgc
    11881 agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg tgatgacggc
    11941 agtggcgttg gccgacagcc ggcacattct cgattgcctg gtggtcaccg atgtgacttt
    12001 cagaacgtta acagacgaag acatcgcggg ctatgtcgcc agcggtgaac cgttagataa
    12061 agcaggtgca tacggtattc aggggctggg tggctgtttt gtcaggaaga taaatggcag
    12121 ctatcaagcc gtagtcggct taccgctggt tgaaacgtat gaattattaa gtaattttaa
    12181 cgcactgcgt gagaaaaggg ataaacatga cggctgaatt gttagtaaac gtaacgcctt
    12241 cggaaacgcg agtggcgtat attgatggcg gtattctgca ggaaattcat attgaacgtg
    12301 aggcgcgacg cggaatagta ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg
    12361 gtatgcaggc ggcttttgta gatattgggc tggataaagc cgcgtttctt catgcatctg
    12421 acatcatgcc gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg
    12481 acatctcgga actggttcgt caggggcaag atctgatggt gcaggtggtg aaagatccgc
    12541 ttggcactaa gggggcgcgc ctgaccaccg atatcacgct gccttctcgc tatctggtct
    12601 ttatgccagg ggcttctcac gttggggttt cccaacgaat tgaaagcgaa tcagaacgtg
    12661 aacgcctgaa aaaagtggtc gcagagtatt gcgacgagca gggcgggttt atcatccgta
    12721 ccgcagcgga aggggttggc gaggctgaac tggcctccga tgccgcttat ctgaaacgcg
    12781 tctggaccaa agttatggag cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg
    12841 aactggcgtt ggcgcagcgt gttctgcgtg atttcgccga tgccgaactg gaccgcattc
    12901 gcgttgactc acgcctgact tacgaagcgt tacttgagtt cacctcggag tacattcccg
    12961 agatgaccag caagctggag cattacaccg gacgccagcc gattttcgat ctctttgatg
    13021 tcgaaaacga aatccagcga gcgctggaac gcaaagttga actgaaatcc ggtggttatc
    13081 tgattatcga ccagaccgaa gcgatgacca ccgtggacat caataccgga gcgtttgtcg
    13141 gtcatcgcaa tctggacgac accattttca ataccaatat tgaagcgacg caggctatcg
    13201 ctcgccagtt acggttgcgt aatctgggcg ggattatcat tattgatttc atcgatatga
    13261 ataatgaaga tcaccgccgc cgagtgctgc actcgctgga gcaggcgttg agcaaagacc
    13321 gggtgaaaac cagcgttaat ggtttttcgg cgctggggct ggtggagatg acgcgtaaac
    13381 gcacccgcga aagcattgag cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg
    13441 gaacggtgaa aaccgtggaa acggtatgct atgaaatcat gcgcgagatt gttcgtgtac
    13501 accatgctta cgactccgac cgtttcctgg tctatgcttc tccggcagta gccgaagcct
    13561 tgaaaggcga agagtcacac tcgctggcgg aagtggaaat tttcgttggc aaacaggtta
    13621 aagtacaaat tgaaccgctc tataaccagg agcagtttga cgtcgtaatg atgtaaacag
    13681 atgctgggcc gccatccggc aaagggtttt tgagtcacat ttttagcaga caaggagtga
    13741 cgggtgaggc gattgccggg gattttactg cttactggag ccgcgctcgt tgtgatcgct
    13801 gccctgctgg ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa
    13861 atcctcaaca aaatagaatc cgcgactggc atgccggtag aagccagtca gctctcagcc
    13921 agctggcaga attttggccc gacgcttgaa gcgcacgaca tccgtgcaga actaaaggat
    13981 ggcggcgaat tttcggttaa acgcgttact ctggcgctgg atgtctggca gagcctgtta
    14041 catatgcgct ggcagtttcg cgacctcact ttctggcagc tgcgctttcg caccaacact
    14101 cctatcacca gcggtggtag cgatgacagt ctggaagcca gtcacatcag cgatctgttt
    14161 cttcgtcaat ttgaccattt cgatctccgc gacagtgaag tcagtttcct gacgccatcc
    14221 ggtcagcgcg ccgagctggc gatcccacaa ctcacctggc tgaacgatcc acgtcgacac
    14281 cgtgcggaag gcctggtaag cctctccagc cttaccggac agcacggcgt gatgcaggtg
    14341 cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg gctccaggcg
    14401 gatgacatcg acctgaagcc gtggctcggt aaatggatgc aggacaatat tgcgctggaa
    14461 acggcgcagt tctcccttga aggctggatg acgatcgaca aaggcgatgt aaccggcggt
    14521 gacgtctggc tgaaacaggg tggtgccagc tggttgggcg agaaggaaac gcatacgctg
    14581 tcggtggata atctgaccgc gcatattacg cgtgaaaatc cgggatggca gttctctatt
    14641 cccgatacac ggatcacgat ggacggcaaa ccctggccga gcggagcatt gacgctggcc
    14701 tggataccgg aacaggacgt tggcggcaaa gacaataaac gcagtgacga actccggatt
    14761 cgcgccagta atctggagct ggcaggcctg gagggcatac gcccgctggc cgcgaaactt
    14821 tcacctgcac tgggtgatgt ttggcgctct acacaaccga gcggcaagat taacactctg
    14881 gcgctggata tcccgcttca ggcggcagac aagacccgtt ttcaggcatc gtggagcgat
    14941 ctggcctgga agcaatggaa attattaccg ggtgcggaac acttctccgg gacgctttcc
    15001 ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa
    15061 acggtattcc gtgcgccact ggaaatcgcc gacggccagg caactataag ctggctgaac
    15121 aatgacaaag gtttccagct ggatgggcgt aatattgacg ttaaagccaa agccgtccat
    15181 gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg aaccctggct gggtattctg
    15241 gctggcatca gtaccgatga tggttcacaa gcctggcgct atttcccgga aaacttgatg
    15301 ggtaaagacc tggttgatta cttaagtggc gcgattcagg gcggtgaagc ggataacgcg
    15361 acgctggttt atggtggcaa tccgcaactc ttcccctata aacacaacga aggtcagttt
    15421 gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc agccggactg gcctgcatta
    15481 actaaccttg gtattgaact ggactttatt aacgacggtt tatggatgaa aaccgatggc
    15541 gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga ctactcaaaa
    15601 gaaaaactgc tgattgacgc tgacattaaa ggtccgggta aagccgttgg cccttacttt
    15661 gatgagacac cgctgaaaga ttctctgggt gcgaccctgc aagaactcca gctcgacggc
    15721 gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt aaccgcgaaa
    15781 ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac cactcgacag caccctgaaa
    15841 aatttgagcg gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc actgacagca
    15901 agctggttta atcagccgtt gaacgtggat ttttccacca aagaaggggc aaaagcctac
    15961 caggtcgcgg tgaatctcaa cggtaactgg caaccggcga aaaccggcgt tctgcctgca
    16021 gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg cattgttctg
    16081 ccttatcatg ctggcgcgac gtataacgta gagctaaacg gcgatttgaa gaatgtgagc
    16141 agtcacttac cttcaccgtt agccaaacct gcgggtgaac cactgccggt aaacgttaag
    16201 gttgatggca atctcaacag ctttgaatta accggacagg ctggtgcgga taatcatttc
    16261 aatagccgct ggttgctcgg tcaaaagctg acgctcgacc gtgctatttg ggcggcagac
    16321 agtaaaacgc tcccgccgtt gccggaacaa agtggtgttg aactcaatat gccgccgatg
    16381 aatggtgccg agtggctggc cctgtttcag aaaggtgcgg cggagagtgt cggtggtgca
    16441 gcgagtttcc cacaacacat aacgttacgt acgcctatgt tgtcgctggg aaatcagcaa
    16501 tggaataacc tgagtattgt ttcgcaaccg acggcaaatg gcacccaggt tgaggcgcag
    16561 gggcgtgaaa tcaacgccac gctggcgatg cgtaataacg cgccgtggct ggcgaatatc
    16621 aaatatcttt attacaaccc gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca
    16681 cctttcccga caacggaacg cattaacttc cgtggctggt cggacgccca aatacgatgc
    16741 gcagagtgct ggttctgggg gcaaaaattc gggcgcattg acagtgatat caccatttct
    16801 ggcaatacat taacgctgac caatggactg attgatactg gtttttcgcg gctcactgcc
    16861 gatggtgaat ggattaacaa tccggggaat gaacgtacct cgctgaaagg aaaactgcgc
    16921 gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca cgacgcccat acggcagtcg
    16981 tcatttaatg tggattacga tttacactgg cgtaaagcac cgtggcagcc agatgaggcg
    17041 acgttgaatg gcatcattca tactcaactg ggtaaaggcg aaattaccga aatcaatacc
    17101 ggacatgccg ggcaattgct gcgcttattg agcgtagatg ccctgatgcg taagctgcgt
    17161 tttgatttca gagacacttt tggcgaaggg ttctattttg actccattcg cagcaccgcg
    17221 tggattaaag acggcgttat gcacaccgac gacacgctgg tggatggcct ggaggcggat
    17281 atcgccatga aagggtcggt aaatctggta cgtcgcgacc tgaatatgga agcggttgtc
    17341 gcaccagaga tttctgcgac agtgggcgtg gctgcggctt ttgcggttaa ccccattgtt
    17401 ggcgcggcag tgtttgccgc cagtaaagtg ctggggccgc tgtggagcaa agtctccatt
    17461 ttgcgctatc acatttcggg tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa
    17521 ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt aattgcccca atctcatagg
    17581 ataatcgttg ccaaaggcca acgagccaga acataaccgt aggctggata gggcgttcac
    17641 gccgcatccg gcagccgtaa aaaatcctct actgcagtaa ctaacgagta gcaaaaacga
    17701 tgagtcttaa cctggtaagt gaacaattgc tagcggcgaa cggcctgaaa catcaggact
    17761 tgttcgtgat cctcggtcaa ctggccgaac gtcgccttga ttatggcgat ctctattttc
    17821 agtcgagcta tcacgaatcc tgggttttag aagaccgcat tattaaagat ggttcttaca
    17881 acatcgatca gggcgttggt gtgcgtgcaa tcagcggtga aaaaaccgga tttgcttacg
    17941 ctgaccaaat cagcctgctg gcgctggaac agagtgcgca agcggcgcgc accatcgccc
    18001 gtgatagcgg tgatggcaaa gtacagacgc tgggcgcggt agagcatagc ccgttgtata
    18061 cctcggtaga tccgctgcaa agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg
    18121 tcgataaggt tgcccgcgaa gcggacaagc gcgtacagga agtgactgcc agcctcagtg
    18181 gtgtctatga attaattctg gttgcggcca ccgacggcac gctagcggcg gatgtccgtc
    18241 cgctggtgcg tctttccgtg agcgttctga tcgaagaaga tggcaaacgc gaacgcggtg
    18301 ccagtggcgg cggcggtcgt tttggttatg agttcttcct tgccgatctc gacggcgaag
    18361 tccgtgcgga tgcatgggca aaagaagcag tacgtatggc gctggtcaat ctttctgccg
    18421 ttgctgcacc agcgggcacc atgccggtag tacttggcgc aggttggccg ggcgtgctgt
    18481 tgcatgaagc ggtaggtcac ggtctggaag gcgacttcaa ccgccgtggc acttcagtat
    18541 ttagtggaca ggtcggggag ctggtggctt cagaactgtg taccgtggtt gatgacggta
    18601 cgatggtcga tcgccgtggt tcggtggcga ttgatgacga aggtacgcca ggccagtaca
    18661 acgtgctgat tgagaacggc attctgaaag gctacatgca ggataaactc aacgcgcgtt
    18721 tgatggggat gacgccgact ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc
    18781 cgcgtatgac caacacctat atgctgccgg gtaaatcgac cccgcaggaa attattgaat
    18841 ccgttgagta cggtatctat gcaccgaact ttggtggcgg tcaggtggat atcacctccg
    18901 gcaaattcgt tttctccact tcagaagcat atctgattga aaacggtaaa gtaacgaagc
    18961 cggtgaaagg cgcaacgttg attggttccg gtatcgaaac catgcagcag atttcgatgg
    19021 ttggcaacga cctgaaactg gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt
    19081 tgccggttgg cgtgggccag ccaacgctga aagttgataa cctgactgtt ggcggtactg
    19141 cgtaataatt tcagtatttt cagaaggata attaatcctt cctcgtgcac gaacggtccc
    19201 ctcgcccctt tggggagagg gttagggtga ggggaacccg ttggcacagg tttgtacaac
    19261 gtaacagtac aatatgaatt acttctcttt cccgcgcccg tgcatctcct gaaacaattt
    19321 accgacctca acaaaataat ccgtcagcga gttgatcacg acctgtacct tcagcggcag
    19381 cttatctttt tcggtatata acgcataaac cgggcgtgga tctgactgat aacgcggcag
    19441 caggatctcc agctccccac gattgatctc gttgatcacc cacatcagcg gcacgtaggc
    19501 gatcccggca cccgccgtta gccagcgcac cagcgtcatc ggatcattag tcacaaatct
    19561 cccttgcggg atcaggcgag tcgagatccc ttccggtgca atcagttcaa attcattgtc
    19621 gggccgcacg ctgtattcaa gccatgaatg gctacttaaa tcggcgggtt tttccggtat
    19681 gccgtattgt gtgagatagc ttttcgcggc gcacaccacc attggcatcg cgccaagacg
    19741 gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg atcaccacat ccagaccgtc
    19801 ggcaatcagg tcgggggctg gaattccggt aaccagattg acgctcaaac ctgggtattc
    19861 tttcagcatt ttggctgtca gcccggcgag aacattttgt gccatagttg aagaacagcc
    19921 aatgcgtagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg
    19981 cacttcatga agcatacgac ggcagccctg gtagtaaatt ctaccggctt cggtcaggcc
    20041 aatgctgcgt gtgctacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac
    20101 cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc
    20161 aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga
    20221 cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg ctaatatatt
    20281 aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga
    20341 cagatgctga aaataataac gcctgctctc tctatacaac caaggtcaac atgagtctgt
    20401 ttcccgttat cgtggtgttt gggctgtcct tcccaccgat attttttgaa ttgcttttat
    20461 cactggcgat tttctggctg gtgtgccggg tacttgtgcc aacaggtatc tacgactttg
    20521 tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc
    20581 gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc
    20641 acggtcgtat tagtcattct ggccttcatc gcaattttta atgcctgggt ctattacacc
    20701 gaatccccct ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac
    20761 gtttctggac tcattaccca ggtgaatgtt cataaccagc tggtgaaaaa aggacaggtt
    20821 ctgttcacca tcgaccagcc gcgctatcaa aaggcgcttg aggaagcgca agccgatgtt
    20881 gcttattatc aggtactggc acaggagaaa cgccaggagg ccggacgtcg taaccgtctc
    20941 ggtgtgcagg cgatgtctcg cgaagagatc gaccaggcca acaacgtact acaaacggtt
    21001 ctgcatcagt tagcgaaagc gcaggcgacc cgcgatctgg caaaactgga tcttgaacgc
    21061 acggtgatcc gcgcgccagc agatggctgg gtgaccaacc tcaacgtcta taccggtgag
    21121 tttattactc gtggatcaac ggcggttgcg ctggtgaaac agaactcttt ctatgtactg
    21181 gcctatatgg aagaaactaa gctggaaggg gtgcgccctg ggtatcgtgc agagatcacg
    21241 ccgcttggca gtaacaaagt gctgaaaggg actgttgata gtgttgccgc tggggtcacc
    21301 aacgccagca gcacgcgtga cgacaaaggg atggcgacca tcgactctaa ccttgaatgg
    21361 gtgcgtttgg cgcaacgtgt tccggttcgt attcgtctcg acaaccagca agagaacatc
    21421 tggcctgcgg gcaccactgc tacagtggtg gtcactggca aacaagatcg cgacgaaagc
    21481 caggattcgt tcttccgtaa aatggcccat cgcctgcgtg agtttggtta atcacgatgg
    21541 atattttctc cattgctaac caacatattc gctttgcggt aaaactggcg accgccattg
    21601 tactggcgct gtttgttggc tttcacttcc agctggaaac gccacgctgg gcggtactga
    21661 cagcggcgat tgttgccgcc ggtccggcct ttgctgcggg aggtgaaccg tattctggcg
    21721 ctattcgcta tcgtggcttt ttgcgcatca tcggcacatt tattggctgt attgccggac
    21781 tggtgatcat cattgcgatg atccgcgcac cattattgat gattctggtg tgctgtatct
    21841 gggccggttt ttgtacctgg atatcctcgc tggtacgaat agaaaactcg tatgcgtggg
    21901 ggctggccgg ttataccgcg ctgatcattg tgatcaccat tcagccggaa ccattgctta
    21961 cgccgcagtt tgccgtcgaa cgttgtagcg agatcgttat cggtattgtg tgtgcgatta
    22021 tggcggattt gctcttttct ccgcgatcga tcaaacaaga agtggatcga gagctggaaa
    22081 gtttgctggt cgcgcaatat caattaatgc aactctgtat caagcatggc gatggtgaag
    22141 ttgtcgataa agcctggggc gacctggtgc gacgcaccac ggcgctacaa ggcatgcgca
    22201 gcaacctgaa tatggaatct tcccgctggg cgcgggccaa tcgacgttta aaagcgatca
    22261 atacgctatc gctgacgctg attacccaat cctgcgaaac ttatcttatt cagaatacgc
    22321 gcccggaatt gatcactgat actttccgcg aattttttga cacgccggta gaaaccgcgc
    22381 aggacgtcca caagcagctc aaacgcctgc gaagagttat cgcctggacc ggggaacggg
    22441 aaacgcctgt caccatttat agctgggtcg cggcggcaac gcgttatcag cttctcaagc
    22501 gcggcgttat cagtaacaca aaaatcaacg ccaccgaaga agagatcctg caaggcgaac
    22561 cggaagtcaa agtagagtca gccgaacgtc atcatgcgat ggttaacttc tggcgaacca
    22621 cactttcctg cattctggga acgcttttct ggctgtggac gggctggact tccggcagtg
    22681 gtgcaatggt gatgattgcg gtcgtgacgt cactggcaat gcgtttgccg aatccacgca
    22741 tggtggcgat cgactttatc tacgggacgc tggccgcgct gccgttaggg ctgctctact
    22801 ttttggtgat tatccctaat acccaacaga gcatgttgct gctgtgtatt agcctggcag
    22861 tgctgggatt cttcctcgga atagaagtac agaaacggcg actgggctcg atgggggcgc
    22921 tggccagcac cataaatatt atcgtgctgg ataacccgat gactttccat ttcagtcagt
    22981 ttctcgacag cgcattaggg caaatcgtcg gctgtgtgct cgcgttcacc gttattttgc
    23041 tggtgcggga taaatcgcgc gacaggactg gacgtgtact gcttaatcag tttgtttctg
    23101 ccgctgtttc cgcgatgact accaatgtgg cacgtcgtaa agagaaccac ctcccggcac
    23161 tttatcagca gctgtttttg ctgatgaata agttcccagg ggatttgccg aaatttcgcc
    23221 tggcgctgac gatgattatc gcccaccagc gcctgcgtga tgcgccgatc ccggttaacg
    23281 aggatttatc ggcgtttcac cgacaaatgc gccgcacagc agaccatgtg atatctgccc
    23341 gtagcgatga taaacgtcgt cggtactttg gtcagttgct ggaagaactg gaaatctacc
    23401 aggaaaagct atgcatctgg caagcgccac cgcaggtgac ggaaccggtt catcggctgg
    23461 cggggatgct ccataagtat caacatgcgt tgaccgatag ttaagtcaaa accgacgcca
    23521 aaagcgtcgg ttttttcatg gctatactta gcgatgcacg gcagaactcg ccgcgaaacg
    23581 tgacggtggc aacagatgaa tatttatacc tttgattttg atgagattga gagtcaggag
    23641 gatttttatc gtgactttag ccaaaccttt ggtctggcga aagataaggt acgcgatctc
    23701 gactcactat gggatgtgtt aatgaacgat gtcctgccgc taccacttga gattgaattt
    23761 gttcatctgg gagagaaaac gcgtcgccgt tttggcgcgt taatattgct gtttgatgag
    23821 gcagaggaag agctggaagg gcatttgcgt tttaatgttc gtcattagcg caaaaaaaag
    23881 cccccgaacc gggggcaata tcgtcggaca agacgatgag ggtttatttg tacagttcag
    23941 ccgtagcgtg ccaggtgtca ccgctacgag cttcagtaat ctggtaggcc gttgcgcctt
    24001 tctcttccgc ttttttgttc agcatttcac gcatatccat tggcgaagac gccacaccac
    24061 ttacggatac ggtcccgatt gcttcacgat tttgtgcttg tgcagcatca atggagtcgg
    24121 cagcgaatgc accgaaagag agaacagaaa gtacgcttaa tgcagcaaca gtggttttga
    24181 ttttcatgat ttttacctcg acataatctt ttagctgggc ctttgtttcg tgacccatat
    24241 cacaaaatca agtatacact aattattgag ctaattaata ccgcgctaa
//
LOCUS       DAZFKY010000060        24087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFKY010000060 DAZFKY010000000
VERSION     DAZFKY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            196..531
                     /locus_tag="ACHKCL_002106"
     CDS             196..531
                     /locus_tag="ACHKCL_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5763073.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            790..1233
                     /gene="yjdN"
                     /locus_tag="ACHKCL_002107"
     CDS             790..1233
                     /gene="yjdN"
                     /locus_tag="ACHKCL_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5763074.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1366..2154
                     /gene="phnC"
                     /locus_tag="ACHKCL_002108"
     CDS             1366..2154
                     /gene="phnC"
                     /locus_tag="ACHKCL_002108"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5763075.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2179..3195
                     /gene="phnD"
                     /locus_tag="ACHKCL_002109"
     CDS             2179..3195
                     /gene="phnD"
                     /locus_tag="ACHKCL_002109"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5763076.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3301..4080
                     /gene="phnE"
                     /locus_tag="ACHKCL_002110"
     CDS             3301..4080
                     /gene="phnE"
                     /locus_tag="ACHKCL_002110"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5763077.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4101..4826
                     /gene="phnF"
                     /locus_tag="ACHKCL_002111"
     CDS             4101..4826
                     /gene="phnF"
                     /locus_tag="ACHKCL_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5763078.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4827..5279
                     /gene="phnG"
                     /locus_tag="ACHKCL_002112"
     CDS             4827..5279
                     /gene="phnG"
                     /locus_tag="ACHKCL_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5763079.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5276..5860
                     /gene="phnH"
                     /locus_tag="ACHKCL_002113"
     CDS             5276..5860
                     /gene="phnH"
                     /locus_tag="ACHKCL_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5763080.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5860..6924
                     /gene="phnI"
                     /locus_tag="ACHKCL_002114"
     CDS             5860..6924
                     /gene="phnI"
                     /locus_tag="ACHKCL_002114"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5763081.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6917..7762
                     /gene="phnJ"
                     /locus_tag="ACHKCL_002115"
     CDS             6917..7762
                     /gene="phnJ"
                     /locus_tag="ACHKCL_002115"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5763082.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7759..8517
                     /gene="phnK"
                     /locus_tag="ACHKCL_002116"
     CDS             7759..8517
                     /gene="phnK"
                     /locus_tag="ACHKCL_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5763083.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8628..9308
                     /gene="phnL"
                     /locus_tag="ACHKCL_002117"
     CDS             8628..9308
                     /gene="phnL"
                     /locus_tag="ACHKCL_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5763084.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9305..10441
                     /gene="phnM"
                     /locus_tag="ACHKCL_002118"
     CDS             9305..10441
                     /gene="phnM"
                     /locus_tag="ACHKCL_002118"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5763085.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10441..10998
                     /gene="phnN"
                     /locus_tag="ACHKCL_002119"
     CDS             10441..10998
                     /gene="phnN"
                     /locus_tag="ACHKCL_002119"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5763086.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10985..11420
                     /gene="phnO"
                     /locus_tag="ACHKCL_002120"
                     /pseudo
     CDS             10985..11420
                     /gene="phnO"
                     /locus_tag="ACHKCL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11422..12180
                     /gene="phnP"
                     /locus_tag="ACHKCL_002121"
     CDS             11422..12180
                     /gene="phnP"
                     /locus_tag="ACHKCL_002121"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5763087.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12257..12586)
                     /gene="yjdP"
                     /locus_tag="ACHKCL_002122"
     CDS             complement(12257..12586)
                     /gene="yjdP"
                     /locus_tag="ACHKCL_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5763088.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12655..14925)
                     /locus_tag="ACHKCL_002123"
     CDS             complement(12655..14925)
                     /locus_tag="ACHKCL_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5763089.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15039..16559
                     /locus_tag="ACHKCL_002124"
     CDS             15039..16559
                     /locus_tag="ACHKCL_002124"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5763090.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16556..17530
                     /locus_tag="ACHKCL_002125"
     CDS             16556..17530
                     /locus_tag="ACHKCL_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5763091.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17549..18490
                     /locus_tag="ACHKCL_002126"
     CDS             17549..18490
                     /locus_tag="ACHKCL_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5763092.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18545..19228
                     /locus_tag="ACHKCL_002127"
     CDS             18545..19228
                     /locus_tag="ACHKCL_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5763093.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19243..20103
                     /locus_tag="ACHKCL_002128"
     CDS             19243..20103
                     /locus_tag="ACHKCL_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO5763094.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20100..21101
                     /locus_tag="ACHKCL_002129"
     CDS             20100..21101
                     /locus_tag="ACHKCL_002129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5763095.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21098..21805)
                     /locus_tag="ACHKCL_002130"
     CDS             complement(21098..21805)
                     /locus_tag="ACHKCL_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5763096.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22096..24081
                     /gene="yjcS"
                     /locus_tag="ACHKCL_002131"
     CDS             22096..24081
                     /gene="yjcS"
                     /locus_tag="ACHKCL_002131"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5763097.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5368 a         7016 c         6740 g         4963 t
ORIGIN      
        1 ttaaccttat cttaaataat aaatggaggc aaaaagatta ttttcttttt gcgtttcctt
       61 tcaaatgaaa acgatcgtcg tctaaaatca gcagtacccc cgacaaactc agggattttg
      121 tgtataattg cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact
      181 tttgaggaaa tccacatgtc attaccacac tgcccaaaat gcaactccga atacacttac
      241 gaagataacg gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct
      301 gcacaggaaa gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc
      361 gacagcgtta ccatcattaa agatctgaag gtgaaaggta gctcttcgat gctgaaaatt
      421 ggcaccaaag tgaaaaacat ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc
      481 gacggttttg gtccgatgaa actgaaatct gagtttgtga aaaagaactg attgtattgt
      541 gatcggtaag ccggataagg cgtttacgcc gcatccggca acagtgccag atgcctgatg
      601 cgacgctcgc gcgtcttatc aggcctacaa attcccgcac cctccgtagg ccggataagg
      661 cgtttacgcc gcatccggca acagtgccga ctgcctgatg cgacgctcgc gcgtcttatc
      721 aggcttccct ctcatctgta taaatttcga actacactta actggcttct cttaactgag
      781 gtcaccatca tgccgttaag tccctacctc tcttttgccg gtaactgctc cgacgcgatt
      841 gcctattatc aacgtacgtt gggcgctgaa ctgctctata aaatcagctt cggcgaaatg
      901 ccaaaatcag cgcaggacag cgccgagaac tgcccttccg gaatgcaatt tcccgatacc
      961 gccatcgccc acgccaatgt gcgcattgcc ggaagcgata tcatgatgag cgatgctata
     1021 ccgtcaggaa aagccagcta ctccggtttt acgctggtgc tcgattcgca acaggtcgaa
     1081 gaaggaaaac gctggtttga taaccttgcc gctaacggaa aaatcgaaat ggcctggcag
     1141 gaaactttct gggcgcatgg ctttggcaaa gtcaccgata aatttggcgt accgtggatg
     1201 attaatgtcg tcaaacaaca accaacgcaa taatccgccg ggaggcccgc cctcccgcac
     1261 tgtcatcgaa ttcccgttaa ctcttcatct gttagtcact tttaattaac caaatcgtca
     1321 caataatccg ccacgatgga gccacttttt tagggaggct gcatcatgca aacgattatc
     1381 tgcgtcgaac aactgagcaa aaccttcaac cagcatcagg cgctgcacgc ggttgatctg
     1441 aatatccatc atggtgaaat ggtggctctg cttgggccgt ccggttccgg caaatccacc
     1501 cttttacgtc acttaagcgg tttgattacc ggcgataaat ccgccggtag ccatatcgag
     1561 ctgctgggcc gcactgtcca gcgcgaaggc cgcctggccc gcgatatccg caaaagccgc
     1621 gccaacaccg gctacatctt ccaacaattc aacctggtga atcgcctgag cgtactggag
     1681 aacgtgctga ttggcgcgct cggcagcacg ccgttctggc gcacctgttt tagttggttc
     1741 acccgcgagc agaaacagcg cgcattacag gcgctgaccc gcgtcggtat ggcgcacttt
     1801 gcgtatcagc gtgtttccac gctctccggc ggccagcagc agcgtgtggc gattgcccgc
     1861 gcgctgatgc agcaggcgaa agtgattctc gctgacgaac cgatcgcctc gctggacccg
     1921 gaatcggcgc gcatcgtgat ggacaccctg cgcgacatca accagaacga cggcatcacc
     1981 gtggtcgtca cgctgcatca ggtggattac gccctgcgct actgcgaacg catcgtcgcc
     2041 ctgcgccagg ggcacgtctt ctacgacggc agcagccaac agtttgataa cgaacgtttt
     2101 gaccatctct accgcagcat caaccgcgtc gaagagaacg cgaaagctgc ctgacatccc
     2161 catcattgag gaaaacgaat gaacgctaag ataattgcct cgctggcctt caccagcatg
     2221 ttcagcctca gcaccctgtt aagcccggca cacgccgaag agcaggaaaa ggcgctgaat
     2281 ttcggcatta tttcaacgga atcacagcaa aacctgaaac cgcaatggac gccgttttta
     2341 caggatatgg agaagaagct gggcgtgaag gtcaacgcct tctttgcccc ggactatgcg
     2401 ggcattatcc agggaatgcg tttcaacaaa gtggatatcg cctggtacgg caacctgtcg
     2461 gcgatggaag cagtggatcg cgccaacggc caagtcttcg ctcagaccgt cgcggcggat
     2521 ggatcgccgg gttactggag cgtgttgatc gtcaacaaag acagcccgat caacaacctg
     2581 aacgatctgc tggcgaagcg gaaagatctc accttcggta atggcgatcc taactccacc
     2641 tctggcttcc tcgtccccgg ctactacgtc ttctccaaaa acaatatctc cgccagtgac
     2701 ttcaagcgca ccgtcaacgc cgggcatgaa accaacgcgc tggccgtcgc caacaagcag
     2761 gtggatgtgg cgaccaacaa caccgaaaac ctcgacaagc tgaaaacctc cgcgccggag
     2821 aagctgaaag aactgaaagt gatctggaag tcaccgctga tcccaggcga tccgatcgtc
     2881 tggcgtaaaa acctttccga aaccaccaaa gacaagatct acgacttctt tatgaattac
     2941 ggcaagacgc cggaagagaa agcggtgctg gaacgcctgg gctgggcgcc gttccgcgcc
     3001 tccagcgacc tgcaactggt gccgattcgc cagctcgcac tgtttaaaga gatgcaaagc
     3061 gtgaaaggca ataaagggct gaatgagcag gacaagctgg caaaaaccac cgagattcaa
     3121 gcgcagctgg atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag ttcggtaagt
     3181 aaagcgatgc agtaaatcgt aggtcggata agacgccccg gtgtcgcatc cgacaatgtg
     3241 caggcgttga tgccggatgc ggtgcaagca ccttatccgg cctacagacc ggagccaaac
     3301 atgcaaacca tcaccatcgc cccacccaag cgcagctggt tctcgcttct gagctgggcc
     3361 gttgtactcg ccgtgttggt cgtctcgtgg cagggcgcgg aaatggcccc gctcacgctg
     3421 atcaaagacg gcggcaacat ggcgaccttc gccgccgact tcttcccgcc cgatttcagc
     3481 cagtggcagg attacctcac cgaaatgctc gtcacgctgc aaatcgccgt ctggggcacc
     3541 gcgctggcgg tggttctctc catccccttc ggcctgatga gcgccgaaaa cctggtgccg
     3601 tggtgggttt accagcccgt gcgccgcctg atggacgcct gccgcgccat taacgaaatg
     3661 gtcttcgcca tgctgttcgt ggtcgccgtc ggcctcggcc cattcgctgg tgtgctggcg
     3721 ctgtttatcc acaccaccgg tgtgctctcc aaactgcttt ccgaagcggt ggaagcgatt
     3781 gaacccggcc cggtggaagg cattcgcgcc accggcgcca acaagctcga agagatcctc
     3841 tacggcgtgc tgccgcaggt gatgccgctg ctgatctcct actccctcta tcgcttcgaa
     3901 tccaacgtcc gctcggcgac cgtcgtcggc atggtcggcg cgggcgggat cggcgtcacg
     3961 ctgtgggaag cgattcgcgg tttccagttc cagcaaacct gcgccctgat ggtgctgatt
     4021 atcgtcacgg tcagcctgct ggatttcctc tctcaacggt tgcgtaagca ctttatctga
     4081 ttagcgaggc attgatagct atgcacttgt ctacacatcc gaccagctac ccaacacgct
     4141 atcaggagat agccgcaaaa cttgagcagg agcttcgtca acactaccgc tgcggcgact
     4201 atcttcccgc cgagcagcag ctggcggcgc gctttgaggt aaatcgccac accctgcgcc
     4261 gcgccatcga ccaactggtg gaaaaaggct gggtacagcg ccgtcagggc gtcggcgtgc
     4321 tggtgctgat gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt agccagaatc
     4381 tgctggatca gggcagccat cccaccagcg aaaaactgct gtctgtgctg cgcccggcct
     4441 ccggtcacgt cgctgacgcg ctggggataa ccgaggggga gaacgtcatc catctgcgca
     4501 ccctgcgccg ggtcaacggc atcgcgctct gtttaatcga ccactacttc gcggacctca
     4561 ccctctggcc gacgctgcaa cgcttcgaca gcggctcgct gcacgatttt ctgcgcgagc
     4621 aaacggggat tgcactgcgc cgcagccaga cgcggatcag cgcccgccgc gcccaggcca
     4681 aagagtgcca gcgtcttgaa attccgaata tgtccccgct gctgtgcgtg cgcaccctta
     4741 accaccgtga cggtgaaagc agcccggcgg agtactccgt cagcctgacg cgcgccgaca
     4801 tgattgaatt cactatggag cactgaatgc acgcagatac cgcgacccgc cagcactgga
     4861 tgtccgtact ggcacacagc caaccggcag aactggcagc ccgcctgaac gcgctgaaca
     4921 tcaccgccaa ctatgaggtg atccgcgccg ctgaaactgg cctggtacag attcaggcgc
     4981 ggatgggcgg caccggcgaa cgcttttttg ccggcgacgc cacgctgacc cgcgccgccg
     5041 tacgtctgac tgacggcacc ctcggctaca gctgggtgct ggggcgcgac aaacagcacg
     5101 ccgaacgctg cgcgctgatt gacgcgctga tgcagcaaac ccgccatttt caaaacttat
     5161 cagaaacgct tattgccccg ctggacgctg accgtatggc acgcattgcc gcacgccagg
     5221 ccgaagtgaa cgccagccgg gtcgacttct ttacgatggt tcgcggagac aacgcatgac
     5281 cctggaaacc gcttttatgc ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt
     5341 aaaggccatg agcgagccgg gcgtgattgt cgccctgcat cagctcaaac gcggctggca
     5401 gccgctgaat atcgccacca ccagcgtgct gctgacgctg gccgataacg acacgccggt
     5461 gtggcttgcc gcgccattga gcaacgatat cgtcagccag agcctgcgtt ttcataccaa
     5521 cgcgccgctg gtcagccagc cggaacaggc gaccttcgcg gtaacggatg aggcgatttc
     5581 cagcgaacag cttaacgccc tttccaccgg caccgccgtt gcgccggaag cgggcgcaac
     5641 gctgatttta caggtcgcca gcctgagcgg cgggcgcatg ctgcgtctca ccggcgcggg
     5701 tattgccgaa gaacgaatga tcgccccgca gctgccggag tgcattctgc acgaactcac
     5761 cgagcgcccg cacccgttcc cgctcggcat cgacctgatc ctgacctgcg gcgagcgcct
     5821 gctggctgtt ccgcgaacca ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg
     5881 ggcgagaagg cgatcgacgc cgcccacgcc ctgcaagaga gccgacgccg gggcgatacc
     5941 gatttgcctg aactgagcgt cgcccagatt gaacagcagc ttaatcttgc ggtggatcgc
     6001 gtgatgaccg aaggcggcat tgccgaccgc gagctggcgg cgctagcgct gaaacaggcc
     6061 agcggcgata acgtcgaagc aattttcctg ctgcgcgcct accgcaccac gctggcgaag
     6121 ctcgcggtaa gcgagccgct cgacaccacg gagatgcgtc tcgaacggcg tatttcagcg
     6181 gtttataaag atattcccgg cggccagctg cttggcccaa cctacgacta cacccatcgc
     6241 ctgctcgatt tcaccctgct ggcaaacggc gaagcgccga cgctgaccac cgccgacagc
     6301 gaacagcagc cgtcgccgca cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt
     6361 gaagaggata gcggcgcaca gccggatgac atcacccgca cgccaccggt ttacccctgc
     6421 tcacgttctt cccgcctgca acagctcatg cgcggcgacg aaggctattt gctggcgctg
     6481 gcctactcca cccaacgcgg ttacgggcgc aatcacccgt tcgcgggtga aatccgcagt
     6541 ggttacatcg acgtgtcgat tgtgcccgaa gagctgggat ttgcggtgaa cgtcggcgaa
     6601 ctgctgatga ctgagtgtga aatggtcaac ggttttatcg acccaccgga tgaatctccg
     6661 cacttcacgc gcggctacgg gctggtgttc ggcatgagcg agcgcaaagc gatggcgatg
     6721 gcgctggtcg accgcgctct gcaagccccg gagtacggcg agcacgcgac aggcccggcg
     6781 caggatgaag agttcgtgct ggcacatgcc gacaacgtcg aagccgcagg ctttgtctcg
     6841 cacctcaaac tcccgcacta cgtcgatttc caggccgaac tggagctact caaacgtctg
     6901 caacaggagc agaaccatgg ctaatctgag cggctacaac tttgcctacc tcgacgagca
     6961 gaccaaacgc atgatccgcc gcgccatctt aaaagcggtg gcgatccccg gttatcaggt
     7021 gccgtttggc gggcgcgaga tgccaatgcc gtacggctgg ggaaccggcg gcattcagct
     7081 taccgccagc gtgattggcg aaagcgacgt gctgaaggtg attgaccagg gcgcggatga
     7141 caccaccaac gccgtgtcga ttcgcaactt ctttaagcgc gtgaccgggg taaacaccac
     7201 cgagcgcacg gacgatgcaa cggtcatcca gacgcgtcac cgcatccccg aaacgccgct
     7261 gaccgaagat cagatcatta ttttccaggt gccgatcccc gaaccgctgc gctttatcga
     7321 gccgcgcgaa acggaaaccc gcaccatgca cgcgctggag gaatacggcg tgatgcaggt
     7381 gaagctgtat gaagatatcg cccgcttcgg tcatatcgcc accacctacg cctatccggt
     7441 gaaggtgaac gggcgctacg taatggaccc gtcgccgatc ccgaaattcg ataacccgaa
     7501 aatggacatg atgcccgccc tgcaactctt cggcgccggg cgtgagaagc gcatctatgc
     7561 ggtgccgccg tttacccgcg tggaaagcct cgatttcgac gatcacccgt tcaccgttca
     7621 gcagtgggat gagccatgcg ccatctgcgg atcgacccac agctatcttg atgaagtggt
     7681 gctggatgac gccggaaacc gcatgtttgt ctgctccgat accgattatt gccgccaaca
     7741 gagcgaggca aaaagccaat gaatcaaccg ttactttcgg tcaataacct gacccacctt
     7801 tacgcgccgg gcaaaggctt tagcgatgtc tcttttgatt tatggccggg ggaagtgctg
     7861 ggcattgtcg gggaatccgg ctccgggaag accacgctgc tgaaatcaat ttccgcgcgt
     7921 ctgacgccgc agcaggggga gattcactat gaaaaccgtt ccctctatgg catgagcgag
     7981 gccgaccgcc gtcgcctgct gcgcaccgaa tggggcgtag tgcatcagca tccgctcgac
     8041 ggcctgcgcc gccaggtctc ggcaggcggc aatatcggcg agcggctgat ggcgaccggg
     8101 gcgcgtcatt acggcgatat tcgcgccacc gcgcagaagt ggctggaaga ggtggaaatt
     8161 cccgccaacc ggatcgacga cctgccgacc accttttccg gcggtatgca gcagcgtttg
     8221 cagattgccc gcaacctggt gacgcatccg aagctggtgt ttatggatga accgaccggc
     8281 gggctggatg tgtcggtgca ggcccgcctg ctcgacctgc tgcgcggcct ggtggtggag
     8341 ctgaacctcg cggtagtgat tgtcacccac gatttaggcg tcgcccgcct gctggcggac
     8401 cgtttgctgg tgatgaagca ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc
     8461 gacgacccgc atcatccgta tacacagctg ctggtgtcat cggttttgca gaattgagcc
     8521 ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga
     8581 taagacgcat cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg attaacgtac
     8641 aaaacgtcag taaaaccttc atcctgcacc agcaaaacgg cgtgcgcttg cccgtcctca
     8701 accgcgcctc gctcaccgtc aacgcgggcg aatgcgtggt gctccacggc cactccggca
     8761 gcggcaaatc aaccctgctg cgctcgctgt acgccaacta tctgcccgat gaagggcaaa
     8821 tccagatcaa acacggtgac gagtgggtag acctggtcac tgcgccagcg cgcaaagtgg
     8881 tggaaatccg caaaaccacc gttggctggg tgagccagtt tctgcgcgtt atcccgcgca
     8941 tttcggcgct ggaagtagta atgcagccgc tgctcgatac cggcgtaccg cgtgaagcct
     9001 gcgccgctaa agccgcgcgt ctgctcaccc gtctgaacgt gccggaacgc ctgtggcgcc
     9061 ttgcgccatc gaccttctcc ggcggcgaac agcagcgcgt caacatcgcc cgtggcttta
     9121 tcgtcgacta ccccattctg ctgctcgacg aacctaccgc ctcgctggac gccaaaaaca
     9181 gcgccgccgt agtcgaactg atccgagaag ccaaagcccg cggcgcagcc atcgtcggca
     9241 ttttccatga cgaagccgta cgtaatgacg tcgccgaccg cctgcaccca atgggagcct
     9301 cttcatgatt atcaataacg tcaaactggt gctggaaaac gaagtggtaa acggttcgct
     9361 ggagatgcag gacggcgaaa tccgcgcctt tgccgaaagc cagagccgcc tgccggaggc
     9421 gatggacggc gaaggcggct ggctgctgcc ggggctgatt gagctgcata cagacaacct
     9481 cgataaattc ttcaccccgc gcccgaaagt tgactggccc gcccactcgg cgatgagcag
     9541 ccatgacgcg ctgatggtgg cgagcggcat caccaccgta ctggacgccg tggcgattgg
     9601 cgacgtgcga gacggcggcg atcggctgga gaatctggag aagatgatca acgccatcga
     9661 agagacgcag aaacgcggcg tcaaccgcgc cgagcaccgc ctgcacctgc gctgcgagct
     9721 gccgcataac accacgctgc cgctgtttga aaagctggtg cagcgcgagc cggtgacgct
     9781 ggtgtcgctg atggatcact cgccgggcca gcgccagttc gccaaccgcg agaagtatcg
     9841 cgaatattat cagggcaaat actcgctcac cgatgcgcag atgcagcagt atgaagagga
     9901 acagctggcg cttgccgcac gctggtcgca gccgaatcgc gaatccatcg ccgcaatgtg
     9961 ccgcgcgcga caaattgcgc tcgccagcca cgatgacgca acgcacgccc acgttgccga
    10021 atctcaccag cttggcagcg tgatcgccga atttcccacc acgtttgaag cagcggaagc
    10081 ctcgcgcaag catggcatga acgtgctgat gggcgcgcca aatatcgtgc gtggcggttc
    10141 gcactccggc aacgtggcgg ccagtgaact ggcacagctt ggcctgctgg atatcctctc
    10201 ttccgactac taccccgcca gcctgctgga tgcggcgttc cgcgtcgccg atgaccagag
    10261 caaccgcttt acgctgccgc aggcggtgaa gctggtgacc aaaaatccgg cgcaggcgct
    10321 taatctccag gatcgcgggg tgattggcga gggcaaacgc gccgacctgg tgctggcgca
    10381 tcgcaagggc aatcacattc atatcgacca cgtctggcgt cagggtaaaa gggtgttctg
    10441 atgatgggaa aactgatttg gttaatgggg ccgtccggct ccgggaaaga tagcctgctg
    10501 gcggaactcc gcctgcggga acaaactcag ctactggtgg cgcatcgcta catcacgcgc
    10561 gacgccagcg ccggaagcga aaaccatatc gccctgagcg agcaggaatt ttttacccgc
    10621 gcggggcaaa atctgctggc cttaagctgg cacgctaacg gtctgtatta tggcgtcggc
    10681 gtcgagattg acctctggct gcacgctgga ttcgacgtgc tggtcaacgg ctcacgcgcc
    10741 catctgccgc aggcgcgggc gcgctatcaa tcggcgctgc tgcccatttg cttacaggtt
    10801 tcgccagaga tcctgcgcca gcgcctggaa aaccgtggcc gcgaaaacgc cagtgaaatt
    10861 aacgcccggc tggcgcgcgc cgcccgctat actccacagg attgccatac gctcaataat
    10921 gacggcagcc tgcgccagtc ggtcgacacg ctgctgacgc taatccatca gaaggagaaa
    10981 caccatgcct gcttgtgagc tttcgcccgg ccacgcagta cgacaccgac gcggtttacg
    11041 cgttgatttg cgagctaaaa caggcggagt ttgaccacca cgcgtttcgc gtgggcttta
    11101 acgccaacct gcgcgacccg aacatgcgct accatctggc gctgctggat ggcgaagttg
    11161 tcggtatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa
    11221 ttcaggagtt ggtggtaatg ccgcaggcgc gcggtctgaa cgtcggcagt aagttactgg
    11281 cgtgggcaga agaagaagcc cgccaggccg gggccgaaat gaccgaactt tcgaccaacg
    11341 tgaagcgcca cgacgcgcac cgtttctatc tgcgcgaagg ctacgagcag agccacttcc
    11401 gcttcaccaa ggtgctgtaa catgagcctg accctcacgc tcaccggctc cggcggcgca
    11461 cagggcgttc cggcatgggg ctgcgagtgt gcggcctgcg ccagagcgcg gcgctcgccg
    11521 cactatcgcc gccagccatg cagcggcgta gtgaagttta acgaggcgat caccctgatc
    11581 gacgccgggc ggcacgatct cgccgatcgc tggtcgcccg gatcgttcca gcagtttttg
    11641 ctgacgcact accatatgga tcacgtgcag gggctattcc cgctgcgctg gggcgtgggc
    11701 gatccgatcc cggtttacgg cccaccggat gaacagggct gcgacgatct gtttaaacat
    11761 ccgggcctgc ttgatttcag ccacacggtg gaaccgtttg tggtgtttga tttgcagggg
    11821 ttacaggtca cacccctgcc gctcaaccac tcaaaactga ccttcggtta tctgctggaa
    11881 acggcgcaca gccgggtggc gtggctgtct gacaccgctg gcttgcccga aaaaacgctg
    11941 aaatttttac gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc
    12001 gaggatgcac cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc
    12061 cgctcgccgc gcgtggttct gacccatatc agccaccagt ttgatgcgtg gctgatggaa
    12121 aacgcactgc cgtcggggtt tgaggcgggg tttgatggga tggagattgg ggtggcgtga
    12181 tgagagggaa tgtgcgtgct ggccccctca ccctaaccct ctccccagag gggcgatgcc
    12241 tgccccatac ccaacatcat tgcccatact catcttccat tctccgctct tcatcttcta
    12301 gctgccgacg ctcctgatta agctggtgct ggcgatcgtc cagctgcctg cgacgatctt
    12361 caaactggcg acggcggtcg tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc
    12421 agccgtcgtc gcgcgaatct tcataatctc gcccacggtc agggttagaa gcgtcgttaa
    12481 tcgcctgctg aatattgcca atggtgtcat cgataatatc ggcctgggcc ggtacggtgg
    12541 tcagcgtgag cagggtgaaa agaaaaagcg tggagtggcg tttcataaac tcggcctcgc
    12601 aacaaagtta atacaaggta ggcaaaggtg cctgttacga gtagaggagg attctcaatt
    12661 ctcttgcccc agcgcctgcg ccaactgcgc acgggtaaag ggtttgcgca acagcgccac
    12721 gtctggcagc gcggggttat ggctggggcg taaatcctga ccgctgatga gtagcagcgt
    12781 caggtgcggg taaagtttgc gggccgcatt gaccacttct gcgccgctca tgccgccggg
    12841 gagcattaaa tcgctgataa aaatatcgat ttctgctgat gccgccagca gatccagcgc
    12901 ctgttcgccg ctgcttgctt ccagcgtcaa atagcccagc aggtgcaact gttcgcagat
    12961 ggtctggcgc accgcggctt catcttccag cactaacacc agtttatcgc cgctgacaga
    13021 agcctgttct actgccgctg gcgataggtt tttcgccgcc gtcagcgcgc ggggaagctg
    13081 taatctgacc gttgtcccct gccccggcgc gctttcaatt tcaacgcgcc caccggactg
    13141 gcgcacaaaa ccatagacca tcgacaaacc cagcccgctg ccgctgcccg tctgtttggt
    13201 ggtaaagaat ggctcaaata cctggctttt caccgcctgc gacataccgc agccatgatc
    13261 gatgacctcc agtgccacca tgtcctgacg ctgcccactg ctgcgagtaa cgcgctgatt
    13321 ccaggtacga attttgatga ttcccgtctg cccttccatc gcgtcacggg cgttcatcac
    13381 caggttgatg atggcatttt ccagttggct gatgtcaatc cacgcgggcc acgccggaga
    13441 ctgcgcttca atctccagcg ccagcgttgc cggtaaggag tggcgcatta attcgcttaa
    13501 ctcttccagc aatgttttca gttcaacggc ttgcggatgt aaggactgct tgcgggaaaa
    13561 cgccagcagt cgttgtgtca gtagcgcccc acgttcggca gcttgcagcg cccgctgtat
    13621 tcgcggcgcg tctggcgagt ccggattcac cagctccagg ctgccaataa tcaccgccag
    13681 cagattatta aaatcatggg ccagcccgcc cgtcagttgc ccgacagctt tcagtttctg
    13741 gctgtgtaat agcgcctctt ccaacccctg ccgctcagtt tgggcaatct ccatctgcgt
    13801 ggtcttctct tttagcagcc gtgaggttcg ttcgagtgac gcggtattgc gggcaaaaac
    13861 gttaaaggcg cgcgccagtt cacccaactc atcacgccgc tgtaacgccg gaaaagtgac
    13921 gtccgcttcg ccgtgcgcca ggcggctcat ggcgcgggaa atcgccgtca ggttagtgcc
    13981 gaggttgcga tagatatacc agccggcaaa cgcggtgatc accaccgcta acagggcgaa
    14041 aatcatgatc accctctcgc cggagcgcaa atcctggtaa ctttgcgcca cacgctgacc
    14101 ggagagcgtc gccacctgtt cggcgtaacg gttgatgtcg ccattgagga tcgccaccag
    14161 cgctttgatg tgaaacatat accagctgat tgccagatcg ctttgctcca gagcggcaga
    14221 aagcggcgcg agtttacgca tttcatcatt gaaatcattg aaatcattga gtaacgtatt
    14281 aaccaatggc gtatcgctgt gggtcggcaa cgcagacatt accccgacca gttgatgaat
    14341 aatcgcctgc ggcgcggggg tggcaatagc ggcaacaatc agtcggttca tctcgttaag
    14401 caaaatatca tcttgcgcgt gagtgacttt ttgcagatgc cgcagataac ttaagttttg
    14461 atacagcgaa cttaacagcg cgttgcgctc cagatgccgc ctttgcccgc gctccagcat
    14521 accgccgacg ctggtttgca gttcgttgct acgcgtgatg attcgcgtca ccagcccggc
    14581 ttcctgctgc gctaaaggcg cgttcgccag ttgtgcaagc gagcttttta atgcccgctg
    14641 tgtctcctgc aaccgctcgg cttcgccttt atattccagc gctcccacca cctgcgacaa
    14701 tcgcaccgcc gccgttgcca cgtttgccgt gtcgcgcgcc agattcaggc tgccggtcat
    14761 atcatccacc gtttgctgct gcaccagctc ctgcatctgg ctggcatgac gaaagcccat
    14821 taccgccacg ccgctgacca tcaacgtcac cgccaccacc agcaaattca aaagcagcaa
    14881 tcgcccgcgc gcgctggcaa aaaagtgacg tccggggtgc gccatatcac gctccgtgaa
    14941 gaaaaatcgc aatatgacaa atatgaacgg cttctgacat tttgcattta ctttcctgtg
    15001 atggagtgct gatatcgaca acctcgcagg agtccaccat gaacagcacg ccagttctgg
    15061 agatgcgcaa tattgccaaa gccttcggca aattttatgc actcaaaggg gtggatttga
    15121 cggtctaccc tggcgagatc catgccctga tgggggaaaa cggcgcggga aaaagcacgc
    15181 tgatgaaggt gctggcgggg gcgtataccg ccaccagcgg cgagattctc atagacggca
    15241 agccctttca cattcgcacg ccaaaagatg ccttaagcgc cggtattacg ctgatttatc
    15301 aggagatgca gctggcaccg aatctgtcgg tggcagaaaa tatttttctc ggcagcgagc
    15361 tttcccacgg cgggctggtg cagcgtaaag agatgctagt gcaggcgcaa aaagtgatcg
    15421 accgcctcgg cgcgcagttt aacgccagcg ataaggtcat gacgctgacc attgccgagc
    15481 aacagcaggt cgaaatcgcc cgcgcactac atcgcaacag ccgcattctg gtgatggacg
    15541 aacccaccgc tgccctctcc tcccgcgaaa ctcaccgcct gtttgaactg attatgcggc
    15601 tgcgcgatga agggatggcg attatctaca ttagccaccg catggcggaa gtgtatgagc
    15661 tttccgatcg cgtcagcgtg ctacgcgacg ggcaatacgt tggcagcctg acccgcgata
    15721 acctcaatgc cggggagctg gtgcggatga tggtcggcag gccactgagc gatctgttca
    15781 ataaagagcg cgatatcccg ctcggtaaag cccgcctgaa tgttcaccac ctgaccgacg
    15841 gcggcaaagt ccagccgagt agcctgctgg tgcgttccgg cgaaattgtt ggcctcgccg
    15901 gactggtggg tgccggacgt tccgaactgg cgcagttgat cttcggcgtg cggaaagcga
    15961 caggcggaat gattgaagtc gatggtgaac cggtggtgat ccactccccg cgcgaagcca
    16021 tcgatcttgg cattggtttt ctcaccgaaa accgcaaaga acaaggctta ttccttgaaa
    16081 tggcagcagc cgaaaacatc accatggcaa ccctggagcg cgatgcccgc tggggaatgc
    16141 tcaatcgcaa aaaagcgcaa accatttccg atgacgccat taagttgctc aacattcgcg
    16201 tgcctcatgc ccaggtacgc gcgggcgggc tttccggcgg caatcagcaa aaactgttga
    16261 tctcccgctg ggtggcgatt ggcccgcgca ttttactgct cgatgaaccc gcccgcggcg
    16321 tggacgttgg cgccaaaagt gagatctacc ggatcatgaa cgagatggcg cgcaagggcg
    16381 tggcgatcct gatgatctcc agcgaactgc cggagatagt gggaatgagc gatcgcgtct
    16441 atgtgatgcg cgaaggcagc attgccggtg agttaaacgg caaaaacatc acccaggaaa
    16501 acattatgac cttagcgact ggcgtcaacg acgcccattc ccaggcggta acctcatgac
    16561 gacctctgca aaaacctctg ccaaaaaaat gctgatgagc gatctgatgc aaacggtcgg
    16621 catcttgccc attttgattc tgatcgtggc ggtatttggc tttatcacgc caaacttttt
    16681 taccgaaagc aacctgctga atattacccg ccaggcatcg atcaacattg ttctggcggc
    16741 ggggatgacc ttcatcattt tgaccggcgg gatcgatctt tccgtgggtt cgatcctcgg
    16801 caccacagcg gtggcggcga tggtcgtctc ccttagccct gagttcgcca tgctgtcgat
    16861 cccggcagcg ctgatgctcg gcctgctgct ggggctgttc aacggcgcgc tggtggcctt
    16921 tgccggattg ccaccgttta tcgttacgct tggcacctat acggcgctgc gcggggcggc
    16981 gtatctgctg gcggacggca ccacggttat caactccaac attaatttcg agtggatagg
    17041 caacaactat ctcgggccgg tcccatggct ggtggtgatt gcgctggcgg tgatcgtggt
    17101 gtgctggttt attctgcgcc gcaccacgct tggcgttcat atctacgccg tcggcggcaa
    17161 tatgcaggcg gcgcgtctta ccggcattaa ggtctggctg gtgctgctgt ttgtttatgg
    17221 catgagcggc ctgctttctg ggcttggtgg gattatgagc gcctcacggc tgtacagcgc
    17281 caacggcaac ttaggcatgg gttacgaact ggatgccatt gcagcggtga tcctcggcgg
    17341 caccagtttt gtcggcggca tcggcaccat caccggcacg ctggtcggcg cgctaattat
    17401 cgccacgctc aacaatggca tgaccttaat gggcgtctct tacttctggc aactggtgat
    17461 caaaggggcg atgatcatca ttgcggtgct gatcgacaaa taccgtaccc gacaccatca
    17521 aagtgcataa acacatgagg aatacagcat gagattgaag cccttagtga ccgcgctctg
    17581 tgcaggcgcg ctgctggccg cagcaccgtt cgcccaggcg aaagaactga aatcaatagg
    17641 cgtaacggtg ggcgatctgg cgaacccgtt cttcgtgcag atcaccaaag gcgcggagct
    17701 ggaagcacgc aaactggcag gcgataacgt caaagtgacg ctggtttcca gcggctacga
    17761 tctgggtcag caggtgtcgc agatagacaa ctttatcgcc gctaacgtcg atatgattat
    17821 cctcaacgct gccgattcaa aagggatcgg cccggcggtg aaacgggcga aagatgccgg
    17881 gatcgtggtc gtcgcggttg acgtagcggc agaaggtgcc gatgccacca tcacttccga
    17941 taacacccag gcaggcgaaa tggcctgtaa atacatcacc gatcgcctga aaggcaaagg
    18001 caacgtggtg atcatcaacg gcccgccggt atccgccgtg caaaaccgcg tcgaaggatg
    18061 ccagactgaa tttaaacgcc atcctgacat caaagtgctc tctgacaacc agaacgcgaa
    18121 aggcagtcgt gaaggcgggc tggaggtaat gacgggactg ctggcggcaa atccgaagat
    18181 cgacggcgta tttgcgatta acgatcccac cgcaatcggt gccgatctgg cagcaaaaca
    18241 ggcacaacgt aacgagttct ttattgtcgg tgtggacgga agcccggacg ccgaagaggc
    18301 gctcaagcgt gagaactcgt tgtttgtcgc tactccggcg caagatcccc aggtgatggc
    18361 agcgaaagcg gtggaaattg gctacgacat tttgcagggc aaacctgcgc cgaaagagcc
    18421 ggtgctgatc ccggtgacga tgatcgataa aaacaacgtc ggcaattaca aaggctggac
    18481 ggtgaaataa gtccacacgc cgggcggcgc agctgcccgg tcaccgctaa ggagaacgct
    18541 ttcgatgaaa cgctccgcta taaacgacat tctcggccat acacggcaat tcttttcgca
    18601 acatgacgta catctgccgc cctttgccag tttctcgcct gctcaatggc agcagctcga
    18661 caccgccgcg tgggaggagg tttttgatct taaactcggc tgggatgtta ccgcttttgg
    18721 tcgcaacaac tttgcagcac acgggctgac gctgtttacc ctacgtaacg gctcagcaaa
    18781 aggaatgccg tatgtgaaat gttacgccga gaagatcatg catgtgcgcg atgcgcaggt
    18841 cacgccaatg cattttcact ggcgtaagcg tgaggacatt atcaatcgcg gcggcggtaa
    18901 tttaattgtt gaactgtgga atgccgatag taacgagcaa acggctgata gtgacattac
    18961 ggtggtgata gacggctgcc gccagaaaca tactgcaggc tctcagttgc gcctgtctcc
    19021 cggcgaaagt atctgcctgc cgcccggcct gtatcacagc ttttgggcag aagccggttt
    19081 tggcgatgtg ctggttggcg aagtctcttc tgttaatgac gacgaccacg ataaccactt
    19141 tttacagcca ctggaccgct acaacctgat tgacgaagac gaacctgcac agttggtatt
    19201 gtgtaacgaa taccgccagt tccgctgaat aaggataacg ttatgccatt gatttctctc
    19261 gctgaagggc tggcacatgc ccgcgaacat cactattcgt taggtgcgtt taatgtgctg
    19321 gactcgcact ttttacgcgc cttgtttgct gctgccagac aagaacgttc gccctttatt
    19381 atcaacattg ccgaagtaca ttttaaatat atctcgctgg attccctggt cgagacagtg
    19441 aaatttgaag cggcgcgtca tgacattccg gtggtgttaa atctcgatca cggactacat
    19501 tttgaatcgg tggtgcaagc gttgcggctg ggattcagct cggtaatgtt tgatggctcc
    19561 acgctggggt atgaagaaaa tgtccgccag acgcgggaag tggtgaaaat gtgccatgcc
    19621 gttggcgtat cggtagaagc tgaactgggc gcggtgggtg gcgacgaagg cggtgcactc
    19681 tatggtcatg ccgatgaatc cctgtttacc gatccgcaac tggcgcgtga ttttgtcgac
    19741 cgtaccggca ttgacgcgct ggccgtggcg attggcaatg cacacggcaa atatcgcggc
    19801 gaaccgaaac tcgatttccc gcgcctcgac gctatccgcc agcaggctgc tatcccgctg
    19861 gtattacacg gcggctcagg gattagcgat gccgatttcc gccgcgccat cgaactgggt
    19921 atccacaaaa tcaatttcta cactggcatg tcgcaagcgg cgctggctgc cgttgaggcc
    19981 cgaatggcgc accgtcagcc gatgtatgac gagtttgccg agttactgct ggcagtggaa
    20041 gccgccatca gcgatacggt tgcacaacag atgcgtattt ttggcagtgc ggggcaggca
    20101 tgatggagcg taaagggatt atcgccgcag gcaatatgtt ggttgaccac gtccatcaga
    20161 ttatccagtg gccagagcgc ggctggctgg cggagatcac ccacagcgaa cgctccaccg
    20221 gcggtgcgcc gcttaacgtc ctgctgacgc tggcgaaaat gcgcaccggg ctgccgttac
    20281 aggcggttgg gctaatcggt caggacagcg acggcgatta catcatggcg atgcttgagc
    20341 aatatcacgt caatcgtcag catgtgcagc gcactacctt tgccccgacg tcgatgtcgc
    20401 aggtgatgac agacccaagc gggcagcgga cgtttttcca ttcgccgggt gcaaacagac
    20461 ttttggatct tccggcgttc gatcagctcg ataacaccat gaagattttc catctcggct
    20521 atttactgtt gctcgacagc ctggatatgc cggacgatgt ttacggcacc cgtagcgcac
    20581 gcctgctggc gcaaatgcgt gatactggct ttgaaacctc actcgacctg gtctcccgta
    20641 agggcgatcc gcgttatcag ccgctggtgc tgcccgcgtt gcgccatctg gattatctgg
    20701 taattaacga actggaggcc ggggagttta gcgggctgga aattcggtta cccaacggcg
    20761 aaccgcatat cgcgcatatt gctgccgccg cgcgtgaact gttagatgcg ggcgtgcgcc
    20821 agcgggtggt gatccactac ccggaagggg cgtggggaga gaccccagaa cagggcggcg
    20881 tgtggatccc ttcgcaaaag ttagaacaac gggaaattat cggcagcgtc ggcgcgggcg
    20941 atgcattttg tgccggattt ttatatggct gccatgaacg ctggacgctt accgagagca
    21001 tcaaactggc acacgcctgc gcgcgggcaa gcctgttatg cgccaatgcc atcgacgggg
    21061 cgaaaacgct ggaagagctg caaacagaaa tgtcggatta atgcacgtcg gcagcgaaga
    21121 cataccccag accgcgcaga gttttaatca acatcggctg atgcgggttg agttcgattt
    21181 tgcgtcgtaa gcgcatgatt aatacgtcga tggtgcgatc aaacacctcc acgctgtcgc
    21241 tgtgggtgaa cgccagcaac tgctcgcggc tcaatacccg acgcgcgttt tgcgccagag
    21301 ccagtaacaa gccatattcg ccctgagtta actcgacatg ttgctgaagc ggattgtgca
    21361 actggcagcg catggtatcg agataccagc cattgaatga cagcccggcg cttttctcgt
    21421 cattaaccgc cagcgcgcca acccgtcgca acaccgcttt cagtcgtgcg accaccaccc
    21481 gcggggtgaa cggtttgcag atgtaatcat ccgcgcccat ttccagcccg accaccacat
    21541 cagactcact gcccatgccg gtcaacatca ccaccggcaa tgtcggacgc agtttctgga
    21601 tttgctgcaa taccctcagg ccgttggtat cgggtagcat catatccagc accaccagcg
    21661 caatgtcagg atcgccctca atacgcaata tggcctcctg tccggtatgg cagactgaaa
    21721 ccgtaaagac atgttcactt aacacgtcct gcaacagtgc gcagatagcc gtgtcatcat
    21781 ccaccaccag cacgacgggt ttcatggtta cactccgcat cattcatctg attaatcatt
    21841 gtgcgtactt cttgtcgaag tgaaagctga aagtaatata aataataaaa atataagtca
    21901 ggtcacatta ttgatgatag taataaatat gtcgtacttt tccctcttca gcacaaatgc
    21961 ctaataaaat ggatcgttcc gctcacactt ccacttatta aagtcgcaat ttcccaatta
    22021 tgataacgcc agctttaaat gacgtatttt gcatggatta catatttacg acataaatga
    22081 taaatggaga tttatatgaa taactctcgg ttattccgtt tgagcaggat tgttattgcg
    22141 ttaactgccg ccagcggcat gatggtaaat accgctaacg cgaaagagga agcgaaagcc
    22201 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
    22261 cgtcaggatt ttgacgatgc ccagcgtgga tttatcgccc cgctgctgga tgaaggtatt
    22321 ctgcgtgatg cgaacggtaa agtttactac cgcgcagacg attacaaatt tgatattaat
    22381 gccgctgcgc cggaaaccgt aaaccccagc ctgtggcgtc agtcgcagat caacggcatt
    22441 tctggcctgt tcaaagtcac cgataaaatg tatcaggtgc gcggccagga tatctctaac
    22501 attacgttcg ttgaggggga gaaaggcatt attgtcatcg acccgctggt gacgccgcct
    22561 gccgcaaaag ccgcacttga cctttacttc cagcatcgtc cgcaaaaacc gattgttgcc
    22621 gttatctaca ctcacagcca caccgaccac tatggtggcg tgaaaggcat tatctctgaa
    22681 gccgatgtta aatccggcaa agttcaggtg attgcccctg ctggctttat ggacgaagcc
    22741 atcagcgaaa acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt
    22801 ctgttactgc cgcacaacgc gcaaggcaac gtgggtaatg gccttggcgt gacgctggca
    22861 acgggcgacc cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg
    22921 attatcgacg gcctggagtt tgacttcctg atgaccccag gtagcgaagc gccagccgaa
    22981 atgcacttct atattccggc cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc
    23041 ctgcacaact tctacactct gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag
    23101 tacctgaacg aaacgctgga tatgtggggt aacgacgcgg aagtactgtt tatgccgcac
    23161 acatggccgg tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatact
    23221 atcacgttca ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa
    23281 atcggcgaca tgattaagct gccgcctgca cttgccaata actgggccag ccgtggctat
    23341 tacggttctg tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac
    23401 ggtaacccgg ctaacctgca tccgtatggt caggtagaga tgggcaaacg ttacgtgaaa
    23461 gcgctgggcg gctctgcccg tgtcatcaac ctggcgcaag aagcgaataa gcaaggtgat
    23521 taccgctggt cggcagaact actgaaacag gtgattgccg ccaacccggg tgaccaggtg
    23581 gcgaagaatc tgcaagcgaa taactttgaa cagctgggct atcaggccga gtccgccaca
    23641 tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg aaggggtgca taagttcagc
    23701 cacggcacca ccggttcccc ggacaccatt cgcggaatgt cggtcgaaat gctgttcgac
    23761 tttatggccg ttcgcctcga tagcgcgaaa gctgcgggta aaaatatcag cctgaacttc
    23821 aatatgagca acggcgataa cctcaacctg acgctgaacg atagcgtgct taactaccgt
    23881 aaaacactgc aaccgcaagc cgacgcctct ttctacatca gccgtgaaga tctgcacgcc
    23941 gtgctgaccg gacaggcaaa aatggcggat ctggtgaaag cgaagaaagc caaaattatt
    24001 ggcaatggcg cgaaactgga agaaattatc gcctgtctgg ataatttcga tttgtgggtg
    24061 aatatcgtaa ccccaaatta atgtcac
//
LOCUS       DAZFKY010000061        24085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFKY010000061 DAZFKY010000000
VERSION     DAZFKY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            97..3849
                     /gene="yfaL"
                     /locus_tag="ACHKCL_002132"
     CDS             97..3849
                     /gene="yfaL"
                     /locus_tag="ACHKCL_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5763098.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3990..4712)
                     /gene="ubiG"
                     /locus_tag="ACHKCL_002133"
     CDS             complement(3990..4712)
                     /gene="ubiG"
                     /locus_tag="ACHKCL_002133"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5763099.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4859..7486
                     /gene="gyrA"
                     /locus_tag="ACHKCL_002134"
     CDS             4859..7486
                     /gene="gyrA"
                     /locus_tag="ACHKCL_002134"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5763100.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7694..9322
                     /gene="yfaA"
                     /locus_tag="ACHKCL_002135"
     CDS             7694..9322
                     /gene="yfaA"
                     /locus_tag="ACHKCL_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5763101.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9319..9942
                     /gene="yfaT"
                     /locus_tag="ACHKCL_002136"
     CDS             9319..9942
                     /gene="yfaT"
                     /locus_tag="ACHKCL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5763102.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9966..14480
                     /locus_tag="ACHKCL_002137"
     CDS             9966..14480
                     /locus_tag="ACHKCL_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5763103.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14481..16130
                     /gene="yfaQ"
                     /locus_tag="ACHKCL_002138"
                     /pseudo
     CDS             14481..16130
                     /gene="yfaQ"
                     /locus_tag="ACHKCL_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16135..16911
                     /gene="yfaP"
                     /locus_tag="ACHKCL_002139"
     CDS             16135..16911
                     /gene="yfaP"
                     /locus_tag="ACHKCL_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5763104.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17186..20035
                     /gene="rcsC"
                     /locus_tag="ACHKCL_002140"
     CDS             17186..20035
                     /gene="rcsC"
                     /locus_tag="ACHKCL_002140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5763105.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20235..20885)
                     /gene="rcsB"
                     /locus_tag="ACHKCL_002141"
     CDS             complement(20235..20885)
                     /gene="rcsB"
                     /locus_tag="ACHKCL_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5763106.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20902..23574)
                     /gene="rcsD"
                     /locus_tag="ACHKCL_002142"
     CDS             complement(20902..23574)
                     /gene="rcsD"
                     /locus_tag="ACHKCL_002142"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5763107.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5888 a         5853 c         6554 g         5790 t
ORIGIN      
        1 attgcatcgg gttggacaat ttacaaaaga aagataaccc atattgtata atgcgtttaa
       61 atatgccgtc ttatataaaa tgatggtcag attaatatgc gggttatctt tctacgcaag
      121 gagtatttat ctctactccc gtcaatgatt gcatctcttt tctctgctaa cggtgtcgcg
      181 gcggccattg atttatgcca gggatatgat atcaaagcga gttgtcacgc cagcaggcaa
      241 agcctttcag gcattacgca ggtctggagt attgccgatg ggcaatggct ggttttttcg
      301 gatatgacca ataatgccag cggtggggcc gtatttttgc aacaaggagc ggaatttaca
      361 ttatcaccag aaaatgaaac tggaatgact ctgtttgcca ataacaccgt ttcaggagaa
      421 tataataacg gcggggcaat atttgctaaa gaaaactcaa cgctgaatct tacggatgtt
      481 attttttctg gtaacgtcgc aggcggctat ggtggcgcaa tctattcttc tggtactaac
      541 gataccggtg ccatcgattt acgtgtcact aacgccgtgt ttcgcaataa catcgctaat
      601 gacggcaaag gtggtgcaat ttataccatc aataatgata tctatttaag tgatgatgtt
      661 tttaacaata accaggcata tacatcaaca agttacagtg atggcgatgg cggcgcaatc
      721 gatgtcacag ataataatag cgacagcaag catccttcag gttatacgat aataaataac
      781 actgccttta caaataacac tgccgaaggt tatggcgggg cgatatatac caatagcgcg
      841 acggctccct atcttattga tatttctgtt gatgacagct acagccagaa cggaggcgtg
      901 ttagtcgatg agaacaatag cgcagcaggc tatggagatg gtccttcctc tgcggcgggt
      961 ggctttatgt atctcggctt aagtgaagtt acctttgata ttgccgacgg aaaaacgctg
     1021 gttattggca atacagagaa tgacggagct gttgactcta ttgctggtac cgggttaatc
     1081 accaaaacag gttccggcga tctggtactt aatgcagata acaatgactt tactggtgag
     1141 atgcagattg aaaacggtga agttaccctg ggccgcagca actccctgat gaatgtcggc
     1201 gatacgcatt gccaggacga tccgcaagac tgctacggtc tgacgatagg gagtattgat
     1261 aagtaccaga atcaggcaga gctaaatgtt ggctccaccc aacaaacctt tgcgcactca
     1321 ttgacgggct ttcagaatgg cactttaaat atcgatgctg gtggcaatgt tactgttaat
     1381 caaggcagtt ttgctggcac catcgaaggt gctggtcagc tcaccattgc gcaaaacggc
     1441 agctatgtgc tggcgggggc gcagtcgatg gcgctaaccg gcgatatagt ggtggatgct
     1501 ggtgcggtgc tttcgctgga aggcgacgcg gcagatcttg ccgctctcca ggacgatccg
     1561 cagtcgatcg tgttaaacgg cggtatgctc gatctctctg atttctccac ctggcagagc
     1621 ggtacatcat acaaagatgg ccttgaagtc agtggcagca gcggaacggt tatcggcagt
     1681 caggatgtgg tagatcttgc aggcggaaac gatatgcata tcggcggcga cgggaaagat
     1741 ggcgtctacg tggtgatcga tgcgggtgac gggcaggtca gcctggcaaa tgacaatcaa
     1801 tacctcggca caacgcaaat cgcttccggt acgctgatgg tgagcgacaa ctcgcagctt
     1861 ggatataccc attataaccg ccaggttatc tttaccgata agccacaaga aagcgtgatg
     1921 gagattactg ccaatgtcga tactcgctct acaacgactg agcatgggcg tgatattgaa
     1981 atgcgcgccg acggtgaagt ggcagttgat gcgggggtag acacgcagtg gggcgcactg
     2041 atggctgaca gcagcgggca gcatcaggat gagggtagca cattgactaa aacgggggcg
     2101 ggtacactgg agctgaccgc cagcggtaca acgcagtcag cggtgagagt agaagagggc
     2161 acgctgcaag gtgatgttgc ggatatcttc ccttatgctt cgtcgctatg ggtcggtgac
     2221 ggggcaacgt tcgttactgg cgcggatcag gatattcagt caattgatgc tacttccagc
     2281 ggcactatcg acatcagcga tggtacggtt ttgcgcctga ccgggcagga tacttccgtc
     2341 gcccttaatg cctcactgtt taactgcgat gggacgctgg tgaatgccac cgatggtgtg
     2401 acgttgacag gtgagcttaa taccaacctt gaaactgaca gcctgactta tctttccaac
     2461 gtgacggtta atggcaatct gaccaatacg tccggtgcgg ttagcctgca aaatggcgtc
     2521 gctggcgata cgctgacggt aaacggtgat tataccggcg gcggtacgct actgctcgat
     2581 agcgaattaa acggcgatga ctcggtaagc gatcaattgg tgatgaacgg taatactgct
     2641 ggcaacacaa ctgtggtggt taactccatt acagggattg gtgagccgac atcgacaggc
     2701 attaaagtgg ttgatttcgc agctgatccc acgcagtttc aaaacaatgc gcagttcagt
     2761 ctggcaggca gcggctacgt caatatggga gcgtatgact acacgctggt ggaagataac
     2821 aacgactggt atctgcgatc gcaagaagta acgccgccat cgccacctga tccagacccg
     2881 actcccgatc ctgatcccac gcaggatcct gatccaacac ccgacccgga acctacgcct
     2941 gcttaccagc cggtgttgaa tgccaaagtt ggcggttatc tcaataacct gcgggcggca
     3001 aatcaggcgt ttatgatgga gcgacgcgat cacgcaggtg gcgatggtca gacgctgaat
     3061 ttacgtgtta tcggcggaga ttatcattac acagcagcgg ggcaactggc tcaacatgaa
     3121 gacacttcta cggtgcaact tagcggcgat ctgtttagcg ggcgctgggg cacggatggc
     3181 gagtggatgc ttgggattgt tggtggctac agcgataacc agggcgacag ccgctcgagt
     3241 atgaccggaa ctcgcgccga taaccagaac cacggttatg cggttgggct gacctcaagc
     3301 tggtttcagc acggtaagca gaagcagggg gcctggctgg ataactggtt gcagtacgcg
     3361 tggtttagca atgatgtttc tgaacatgaa gatggcgtgg atcattacca ttcgtcgggg
     3421 attatcgcct cgctggaagc ggggtatcag tggttaccgg ggcgtggtgt ggtgattgaa
     3481 ccgcaggcgc aggtgattta tcagggcgtg cagcaggatg attttaccgc cgctaaccgt
     3541 gcgcgcgtgt cacaatcgca gggtgatgat attcagacgc ggctgggttt acacagcgaa
     3601 tggcgtaccg ctgttcatgt cataccaaca ttagatctga attattatca cgatccccat
     3661 tcgacggaaa ttgaagaaga tgccagcact atcagtgacg atgcggtgaa gcaacggggt
     3721 gaaataaaag tgggagtcac gggcaatatc agtcagcgag tttcgctgcg tggtagcgtg
     3781 gcgtggcaga aagggagtga tgattttgcc cagacggcag ggtttttgtc gatgacggtg
     3841 aaatggtaat cgctgtctga ccatcactat tgctggatgc ggcgtaaacg cctaatcagg
     3901 cctacggaga gcatttgtag gcctgataag acgcggcaag cgtcgcatca ggcattggcg
     3961 cggttgccgg atgaatccaa acaacaacct cacttattct gcgtgtgcag catatagttc
     4021 acatccacgc cggggccgag tttaaaagta ttagtgatcg ggttgtaatg tagcccggtt
     4081 atatgtcgct ctttaaggct tgtctgatct acccagccca gcagttctgc cggtttaata
     4141 aacttcttca catcatgtgt acctttaggc accatgcgca aaatatattc cgcgccgact
     4201 accgccatca gccatgactt gccgttgcgg ttaagcgtcg agaaaaagac atcgccgccc
     4261 ggtttcacca gttgcgcaca ggctctgacc actgactgcg gatcagggac gtgctccagc
     4321 atctccatgc aggtcaccac atcatactgc ccggcatgtt ttgccgcgtg ctcttccacg
     4381 gtttcctgca cgtaatccac ctgaatgccg ctttccagcg cgtgcagctt tgccacctgc
     4441 aatggctcaa agcccatatc cagaccggtc accgtcgcgc cttcgcgcgc catactctcg
     4501 gccagaatgc cgccgccaca accgacatcg agcacctttt tgccaaataa gccgccagca
     4561 cgctcggcaa tatagctcag acgcagcggg ttaatgcggt gcagcggttt gaactcacct
     4621 tccagatccc accagcggga ggcgacggct tcaaatttag cgatctcttc gtggtctacg
     4681 ttatggttta ccggcgattt ttcggcattc attggcactt ctactccgta attggcaaga
     4741 caaacgagta tatcaggcat tggatgtgaa taaagcgtat aggtttacct caatctgcgc
     4801 ggctgtgtta taatttgcga cctttgaatc cgggatacag tagagggata gcggttagat
     4861 gagcgacctt gcgagagaaa ttacaccggt caacattgag gaagagctga agagctccta
     4921 tctggattat gcgatgtcgg tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct
     4981 gaagccggta caccgtcgcg tactttacgc catgaacgta ctaggcaatg actggaacaa
     5041 agcctataaa aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg
     5101 tgactcggca gtttatgaca cgatcgtccg tatggcgcag ccattctcgc tgcgttacat
     5161 gctggtagac ggtcagggta acttcggttc catcgacggc gactctgcgg cggcaatgcg
     5221 ttatacggaa atccgtctgg cgaaaattgc ccatgaactg atggccgatc tcgaaaaaga
     5281 gacggtcgat ttcgttgata actatgacgg cacggaaaaa attcccgacg tcatgccaac
     5341 caaaattcct aacctgctgg tgaacggttc ttccggtatc gccgtaggta tggcaaccaa
     5401 catcccgccg cacaacctga cggaagtcat caacggttgt ctggcgtata tcgatgatga
     5461 agacatcagc attgaagggc tgatggaaca catcccgggg ccggacttcc cgacggcggc
     5521 aatcattaac ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg gcaaggtata
     5581 tatccgcgcc cgtgctgaag tggaagttga cgccaaaacc ggacgtgaaa ccattatcgt
     5641 ccacgaaatc ccgtatcagg taaacaaagc gcgcctgatc gagaagattg cggaactggt
     5701 aaaagaaaaa cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca aagacggtat
     5761 gcgcatcgtg attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta
     5821 ctcccagacc cagttgcagg tttctttcgg tatcaacatg gtggcattgc accatggtca
     5881 gccaaagatc atgaacctga aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt
     5941 ggtgacccgt cgtactattt tcgaactgcg taaagctcgc gatcgtgctc atatccttga
     6001 agcattagcc gtggcgctgg cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc
     6061 gacgcctgca gaagcgaaaa ctgcgctggt ggctaatccg tggcagctgg gcaacgttgc
     6121 cgcgatgctc gaacgtgctg gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt
     6181 cggcgtgcgt gatggtctgt actacctgac cgaacagcaa gctcaggcaa ttctggatct
     6241 gcgtttgcag aaactgaccg gccttgagca cgaaaaactg ctcgacgaat acaaagagct
     6301 gctggatcag atcgcggaac tgctgcgtat tcttggtagc gccgatcgtc tgatggaagt
     6361 gatccgtgaa gagctggagc tggttcgtga acagttcggt gacaaacgtc gtactgaaat
     6421 caccgccaac agcgcagaca tcaacctgga agatctgatc acccaggaag atgtggtcgt
     6481 gacgctctct caccagggct acgttaaata tcagccgctt tctgaatacg aagcgcagcg
     6541 tcgtggcggg aaaggtaaat ctgccgcacg tattaaagaa gaagacttta tcgaccgact
     6601 gctggtggcg aacacgcacg accatattct gtgcttctcc agccgtggtc gcgtctattc
     6661 gatgaaagtt taccagttgc cggaggccac tcgtggcgcg cgcggtcgtc cgatcgtcaa
     6721 cctgctgccg ctggagcagg acgaacgtat caccgcgatc ctgccggtga ccgagtttga
     6781 agaaggcgtg aaagtcttca tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac
     6841 cgagttcaac cgtctgcgta ccgccggtaa agtggcgatc aaactggttg aaggcgatga
     6901 gctgatcggc gttgacctga ccagcggcga agacgaagta atgctgttct ccgccgaagg
     6961 taaagtggtg cgctttaaag agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt
     7021 tcgcggtatt cgtttaggcg aaggtgataa agtcgtctct ctgatcgtgc ctcgtggcga
     7081 tggcgcaatc ctcaccgcaa cgcagaacgg ttatggtaaa cgtaccgcag tggcggaata
     7141 cccgactaag tcgcgtgcga cgaaaggggt tatctccatc aaggttaccg aacgtaacgg
     7201 tttagttgtt ggcgcggtac aggtagatga ctgcgaccag atcatgatga tcaccgatgc
     7261 cggtacgctg gtacgtactc gcgtttcaga aatcagcatc gtgggccgta acacccaggg
     7321 cgtgatcctc atccgtactg cggaagatga aaacgtagtg ggtctgcaac gtgttgctga
     7381 accggttgac gaggaagatc tggataccat cgacggcagt gccgcggaag gggacgatga
     7441 aatcgctccg gaagtggacg ttgacgacga gccagaagaa gaataatttt acttcttcat
     7501 gccaaaaggg agccatctcc cttttttgaa ttgaaaagtc caggctgcaa agtctgggct
     7561 tttgtcgtat tagggcgcgg taaagtttgg ctgtgtccgt aaaaaatggc tggctataca
     7621 caaggaatgt ggcaatgagt ggtgaaaaaa agcgaaaggc tggcggttct atggtcttgt
     7681 aggttttggc gcaatagcac tgctttccgc tggcgtctgg gcgttgcaat atgctggcag
     7741 tgggccagaa aaaacgttgt cgccgctggt ggtgcacaat aatttgcaaa ttgatctcaa
     7801 tgagccggac ctctttctcg acagcgattc tctaagccag cttcccaaag atctcctcac
     7861 cattccgttt ctccacgatg ttctgagtga agatttcgtt ttctattatc agaatcatgc
     7921 cgatcgtctg ggcattgaag gcagcattcg tcgcattgtc tatgaacacg atctcacgct
     7981 gaaagataag ctcttttcgt cactcttaga tcagcccgcg caggcggcgc tatggcacga
     8041 taaacaaggc catctttcac attacatggt gctgatccaa cgcagtggtt taagcaaact
     8101 gctggagcca ttgttatttg ccgctaccag cgacagccag ttaagcaaaa cggaaatcag
     8161 tagcatcaag ataaatagtg aaactattcc tgtttatcag ttgcgctata acggcaataa
     8221 cgccctgatg ttcgcgacat atcaggacaa gatgctggtg ttttccagca cggatatgct
     8281 gtttaaagat gatcagcagg ataccgaagc cacggcgatc gcaagtgatt tgttgagcgg
     8341 caaaaaacgc tggcaagcca gctttggcct ggaagagcgt gctgccgaaa aaacgccagt
     8401 acgccagcgc atcgtggcca gcgccaggtt gctgggtttt ggctaccagc ggttaatgcc
     8461 ttcttttgct ggcgtacgct tcgaaatggg taacgacggc tggcacagtt ttgtggcgtt
     8521 aaatgatgaa tccgccagcg tagatgccag tttcgatttt acgccggtct ggaacagtat
     8581 gcctgccggg gccagcttct gtgtggcggt gccgtattca cacggtattg ccgaagagat
     8641 gctttcgcac atcagccagg aaaacgacaa gttgaatggg gcgttggacg gtgccgcggg
     8701 gctgtgctgg tatgaagact caaaattgca aaccccgctg tttgtcggtc agtttgatgg
     8761 cactgccgaa caggcgcaat tgccagggaa actgtttacg caaaatattg gtgcgcacga
     8821 aagcaaagcg ccagaaggtg ttttgccggt aagccagact cagcagggcg aagcgcaaat
     8881 ctggcgtcgc gaagtgagtt cccgatacgg tcagtatccg aaagcgcagg cggcgcaacc
     8941 cgatcaatta atgtcggatt attttttccg cgtgtcgctg gcgatgcaaa acaaaacgct
     9001 gcttttctcc ctcgatgaca cgctggttaa taacgccctg caaacactga ataaaacccg
     9061 cccggcaatg gtggatgtaa tacccactga tggcatcgtt ccgctctata tcaatccaca
     9121 aggcgtggcg aaactgctgc gtaacgaaac gttgaccagt ctgccgaaga atctcgaacc
     9181 ggttttttat aacgccgcac aaactttatt aatgccgaag ctggacgctt tatctcaaca
     9241 accgcgttat gtcatgaagc tggcccagat ggaacccggt gccgcctggc agtggctacc
     9301 cataatctgg cagccgctat gaggcacggg ctgctggcgc tgatttgctg gctgtgttgt
     9361 gttgttgccc atagcgaaat gctgaatgtc gaacaatccg ggctgtttcg cgcctggttt
     9421 gtacgcattg cacaagaaca gctccgccag gggccaagtc cacgctggta tcagcaggat
     9481 tgtgcgggcc tggtgcgatt tgcggcgaac gagacgctga aagttcacga cagtaaatgg
     9541 ctaaaaagta acggtttatc tagccagtat ttgccgccag agatgacgct aacacctgaa
     9601 cagcgtcaac tggcgcaaaa ctggaatcag gggaacggga aaaccggccc ctacgtgacc
     9661 gcgattaatt tgattcagta caacagccag tttattggcc aggacataaa ccaggcgctg
     9721 cctggcgata tgattttttt cgatcagggc gatgcccagc acttaatggt ctggatgggg
     9781 cgttacgtca tctaccacac cggaagcgcc acgaaaactg acaacggaat gcgcgcagtc
     9841 agtctgcaac aacttatgac atggaaggac acccgatgga tacccaacga ttccaatccc
     9901 aatttcattg gcatttatcg tttaaatttt ctggcgcgat agcagcgtgc ttgtccctgt
     9961 ctcttgtggg aacagggctt gctaatgctg atgattcgct tccttccagt aactatgcgc
    10021 cgcccgccgg gggaacattc tttttgcttg ctgacagcag ttttagcagc agtgaagagt
    10081 cgaaagtgcg actggaagcg ccggggcgtg attatcggcg ctatcagatg gaagagtacg
    10141 gcggcgtgga cgttcgcctg tatcgtattc ctgacccgat ggcatttttg cgccagcaga
    10201 aaaacctgca tcgcattgtg gtgcaaccgc aatatctggg cgacgggctg aacaatacgc
    10261 tgacctggct gtgggataac tggtacggca aatctcgccg cgtgatgcag cgtactttct
    10321 cttctcagtc acggcagaat gtgactcagg cattacccga attacagctc ggcaatgcca
    10381 ttattaaacc ttcccgttat gtacagaaca accagttttc cccgctgaaa aaatatcccc
    10441 tggtggaaca gttccgttat ccactatggc aggctaaacc ggtcgagccg cagcaagggg
    10501 taaaactgga aggcgcatcc agcaatttca tctcgccgca gccgggtaac atttatattc
    10561 ctctcggcca acaagagccg ggactgtacc tcgtcgaggc gatggttggt gggtatcggg
    10621 cgacgacggt ggtgtttgtt tccgataccg tggcgcttag caaagtgtca ggcaaagagc
    10681 ttctggtgtg gaccgcgggt aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt
    10741 ggactgacgg tcttggcgtg atgacccgcg gtgtgaccga tgacagcggt accttgcagt
    10801 tacaacatat atcgccagaa cgttcataca ttctgggtaa ggatgctgaa ggcggcgttt
    10861 ttgtctccga gaacttcttc tacgaaagcg aaatctacaa cacccgcttg tatattttta
    10921 ccgatcgccc gctatatcgc gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt
    10981 tccacgatcc gttgcattca tcccccatcg tcagcgcccc ggcgaagctt tcggtgctgg
    11041 acgctaacgg cagtctgttg caaaccgtcg atgtcacgct ggatgcgcgc aatggcgggc
    11101 agggaagttt ccgcctgcca gaaaatgccg ttgccggagg ttatgagtta cgtcttgctt
    11161 accgcaatca ggtctatagc agcagttttc gcgtggcaaa ctacatcaag ccacatttcg
    11221 agattggttt agctctcgac aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac
    11281 tgcaacttct ctacccggat ggcgagccgg taaaaaatgc ccgcgtgcag ttaagtttgc
    11341 gcgctcagca attatcaatg gtcggtaacg atttgcgtta tgccggacgt ttccccgtgt
    11401 cgctggaagg cagcgaaacg gtgtccgacg ccagcggtca tgtggcgtta aatctccccg
    11461 ccgccgataa accgagccgc tatttgttaa ccgtctccgc cagtgacggc gcggcgtatc
    11521 gcgtcaccac caccaaagag atcctcattg aacgcggcct ggcgcattac tctttaagta
    11581 ccgccgcaca atacagtaat agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat
    11641 cttcaaaaca ggttcctgtt acgtatgaat ggttgcgtct cgaagaccgc acgagccata
    11701 gcggagatct accgtcaggc ggcaaatcct ttaccgtcaa tttcgataaa cctggcaact
    11761 acaatctgac gttacgcgat aaagacggct taattctcgc cgggttaagc catgccgtca
    11821 gcggtaaggg cagtatgtcg catactggta cggtagatat cgtggcagat aaaacgctgt
    11881 accagcctgg cgaaaccgcg aagatgctga ttaccttccc ggagccaatt gatgaagcat
    11941 tattgacgct ggaacgcgat cgcgttgaac agcagtcgct gctatcgcat ccggcaaact
    12001 ggctgacgtt acaacgttta aacgatactc aatatgaagc ccgtgttcca gtgagcaatt
    12061 cctttgcgcc taacatcact ttttcggtgc tgtatacccg taacggtcag tacagttttc
    12121 agaacgccgg gatcaaagtt gccgttcccc agctggatat ccgggtgaaa acggacaaaa
    12181 cccattacca gcctggtgaa ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac
    12241 ctgtttctgc gcagctaacg gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccag
    12301 aaatcgcgcc gaatatcggc aaatttttct atccgctggg gcgtaacaat gtgcgtacca
    12361 gctccagtct gtcgtttatc agctacgacc aggcactctc cagcgagccg gttgcgcctg
    12421 gcgcaactaa ccgcagcgag cggcgagtaa aaatgcttga acgtccacgg cgtgaagagg
    12481 tggataccgc ggcatggatg ccgtcactca caaccgataa acaaggcaaa gcgtatttca
    12541 cgttcctgat gcctgattcg ttaacccgct ggcgtatcac cgcgcgtggg atgaacggcg
    12601 acgggctggt cgggcagggg cgtgcttatc tgcgttcgga aaaaaatctc tacatgaagt
    12661 ggagtatgcc aacggtgtat cgcgtgggcg acaaaccgtc ggcaggactg tttatcttca
    12721 gtcagcagga taacgaaccg gtggcgctgg tgactaaatt tgcaggcgct gagatgcgcc
    12781 agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac attcagcaat
    12841 ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc attagcacaa
    12901 aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc atgctcggcg
    12961 gtggcgataa cgcgctgatg ttgcccgagc aggcgagcaa tatccggcta caaagtagtg
    13021 aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgaa ccgtgggggg
    13081 gggtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg
    13141 atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac cgtctgcggc
    13201 tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat ggcaatggtg
    13261 acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag gcgctcggcg
    13321 taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag caggcagata
    13381 acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg aatctgccgt
    13441 gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact aaaactgaag
    13501 atttctctga ggaagacacc cgcgatatca atgacagcct gatcctcgat acaccggaat
    13561 ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt
    13621 tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg gtaaacagca
    13681 atcagccgct ggcacacacg gttgtgctgc tcaatagcgg gggcgacgct acccaggcgg
    13741 ctgctatttt aagtggtttg accgctgagc aatctactat tgagcgcgcg ctggccatga
    13801 actggctggc gaaatatatg gcgacaatgc cttcggttgt gttgcctgcg cctgcgggcg
    13861 catgggccaa acataagtta actggagggg gcgaatactg gcgttgggtt ggtcagggcg
    13921 tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc
    13981 gtgaagcggc aaaaacggct caacaaagta acattccggt gaccgttgaa cgccaattgt
    14041 atcggcttat ccctggtgaa gaagagatga gctttactct gcaaccggtg accagcaatg
    14101 agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag caggatgcag
    14161 ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt gagcgcacaa
    14221 catggggcat ttcagtcaat aaacccaacg ctggaaaaca gcaggggcaa ttgctggaaa
    14281 aagcgcgaaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa
    14341 cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg
    14401 ctcgttatgt gcgttcctat gcacctgcac agcaaagtgt tgcggcaggg agcgaatgga
    14461 ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg gcgttggtta
    14521 ctctgccaac gcttgcggag gagacgccgt tacaactggc gctgcgcggt gcgcagcacg
    14581 atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgacacgc
    14641 tcaccacgcc gttagggagt ttatggaaac tgtatgttta cgcctggctg gaggataccc
    14701 atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc
    14761 aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg ctctattttg
    14821 cgccgcagcg gttgcatatt ggggcagatg tgtgggggca atactggcaa cagcgtcagg
    14881 cacctgcgtg gttagcgtct ctttctacgc tgaagccaga aacctcggta acggttaaat
    14941 cgctgcttga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa gtcttactgg
    15001 atgtggtgct ggacgaggcg aaaatcggtg tcgcatctat gctcggtagt cgggtacggg
    15061 tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgtcagggt ggttttgctg
    15121 gctggttaac cgatggtaca ccgctgtggg tgactggcag tggcaccagc aaaacggtac
    15181 tgacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcaa gttgccagtg
    15241 ggcaatgtgt agaggtggag ctatttgcgc gctagccgct gaagaaaatc acagcggaga
    15301 agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg acgtttgcca
    15361 atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgacc gaaaagggca
    15421 aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg
    15481 aggcgaaaag tacgccacct gaggcagcga aagccatgac tgtggcgata cgcacttttt
    15541 tacagcaaaa cgccaatcgg gaaggcgatt gcctgaccat ccccgacagc agcgccacgc
    15601 agcgtgtttc tgcttcgcca gctaccacgg gggcgcgaac gatgaccgtc tggacgcagg
    15661 atctcatcta tgcgggcgat ccggttcatt atcacggcag tcgcgctacc gaagggacgc
    15721 tttcctggcg gcaagccatg gcccaggcgg ggaagggaga gcgttacgac cagatactcg
    15781 cgtttgctta tcccgacaac agccttagcc gctggggagc gccacgcaca acctgtcagt
    15841 tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac
    15901 aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt
    15961 tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg
    16021 atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat cccaccgggc
    16081 ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga
    16141 aagatttttc ttccgttgtt actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg
    16201 caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg cgaagatgcc
    16261 agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat
    16321 atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag
    16381 ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga tggttcattt
    16441 gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccggatggg
    16501 caaagccgac aaaaaatgca gttttactca acgccgggta caggaacgat tcgtgcacgt
    16561 ttacggctgg ttctctcgtg ggatacggac aataccgacc tcgatcttca cgttgtcacg
    16621 cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg tgcattggat
    16681 atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc agttcacggg
    16741 cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt aaccaccgcc
    16801 caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac atttattgta
    16861 ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg atccatccct
    16921 tcacacaacc tggctctttc ttttttcaga taagactgct gggcgatctg ccgacgccag
    16981 gatataacca ttaacaagcg tcggcagatt ggtcatttct tatttttcgt actgagaact
    17041 gtccatttac tccggctttc ctggttaagg caatggtttc taagcgatac gcattattgc
    17101 aatggtgaca gaacattgcg gtaacacgct tttaccgcta ccttaaccac actccatcgg
    17161 tcacctgagg cggagcttcg cccctttgaa ataccttgct tcttttcgta caaccctgaa
    17221 agcctcgcgc tacatgttca gagcattggc gttagtgctc tggctgttga ttgctttttc
    17281 atccgttttt tacatcgtta atgcgttaca tcagcgagaa tcggaaattc gtcaggaatt
    17341 taatctgagt tccgatcagg ctcagcgctt tattcaacgc acctctgatg tgatgaaaga
    17401 gctgaagtac atcgccgaaa atcgcttatc ggcagaaaac ggtgtgcttt ccccgcgtgg
    17461 acgagaaacg caggcggatg tgcctgcgtt tgaaccgctg tttgccgact ccgattgttc
    17521 agcaatgagt aacacctggc gaggttctct ggagtcattg gcgtggttta tgcgctactg
    17581 gcgcgataat ttttctgcgg cttacgatct caaccgggta tttttaatcg gcagcgataa
    17641 cctctgcatg gccaatttcg gtctgcgtga tatgccagtg gaacgcgata ccgcgttgaa
    17701 agctttgcat gaacgcatca ataaatatcg aaatgcacca caagatgata gcggcagtaa
    17761 cctctactgg atcagcgaag gtccgcgccc tggcgtcggg tatttttacg cgttgacgcc
    17821 agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc gagcagacca tccggatgga
    17881 gaactttttc ttaccgggta cgttgccgat gggggttacc attcttgatg aaaatggtca
    17941 taccctgatt tcgcttaccg ggccagaaag caaaattaag gccgatcctc gctggatgca
    18001 ggaacgctcc tggtttggct atacggaagg gttccgggag ctggtgctga agaaaaatct
    18061 gccaccctca tcgctcagca tcgtgtattc ggtgccggtt gataaggtgc tggaacgcat
    18121 tcgcatgttg atccttaacg caattttgct gaatgtgctt gccggagctg cattgtttac
    18181 tctcgcgcgg atgtacgagc gacgtatttt cattccggcg gaaagcgacg ccctgcgact
    18241 ggaagaacat gagcagttca atcgcaagat tgtcgcctcc gcgccagtgg gtatctgcat
    18301 tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa ctggcgcata cctatctcaa
    18361 tatgcttacg catgaggacc gccaacgact gacacaaatt atctgtgggc agcaggtcaa
    18421 ttttgttgat gtcctgacca gcaacaatac caatctgcaa atcagcttcg tccattcgcg
    18481 ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat gtttcttcgc gcgtgaagat
    18541 ggaagagtcg ttgcaggaga tggcacaagc agcggaacag gcgagccagt caaaatcgat
    18601 gttccttgcc accgtcagtc atgagctgcg aacgccgctg tatggcatta tcggtaacct
    18661 ggatctgttg caaaccaaag agttaccgaa aggcgtcgat cgtctggtga cggcaatgaa
    18721 caactcttcc agcctgttgt tgaaaattat cagcgatatt ctcgatttct cgaagattga
    18781 atcggaacag ttgatgatcg aaccgcgtga gttttcaccg cgtgaagtga tgaaccacat
    18841 caccgccaac tatttaccgc tggtggtacg caagcagtta ggcttgtact gctttattga
    18901 accggatgtg ccagtggcct taaatggcga cccgatgcgt ttacagcagg tcatctccaa
    18961 cctgttgagt aacgccataa aattcaccga taccggctgt atagttttgc atgtccgcgc
    19021 tgatggcgat tatctctcta tccgtgttcg cgataccggc gtggggattc cggcgaaaga
    19081 agtggtgcgc ttgtttgatc ccttcttcca ggtcggaacg ggcgtacagc gaaacttcca
    19141 ggggaccggt ctgggtctgg cgatttgtga aaaactgatc agcatgatgg acggcgatat
    19201 ctcggtagat tcagaaccgg gaatgggcag ccagtttacc gtgcgtattc cgttgtacgg
    19261 cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt ggtaaacgct gctggctggc
    19321 ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt ttgcagcgca gcggcatcgt
    19381 cgtaacaaca tacgaagggc aggaaccgac tcccgaagat gtgttaatca ctgacgaggt
    19441 agtgagtaaa aaatggcagg gcagagcggt agtgaccttc tgtcgtcgac atattggtat
    19501 tccgctggag aaagcgccag gggagtgggt acacagtgtg gctgctccgc atgagctacc
    19561 ggcattgttg gcgcgtattt atttgatcga gatggagagc gacgatcctg ctaacgctct
    19621 gccgtcgacg gacaaagcgg tcagcgataa tgacgatatg atgattctgg tcgtggatga
    19681 tcatccgatt aaccggcgtt tgctggcaga tcagttggga tcgttgggct atcaatgtaa
    19741 aaccgcgaat gatggcgtcg atgcgcttaa tgtacttagc aagaatcata ttgatatcgt
    19801 gcttagcgac gtcaacatgc caaatatgga tggttaccgc ttgacacaac gcattcgtca
    19861 gttgggactg acgttgccgg taatcggagt aacggctaat gcgttggctg aagagaagca
    19921 gcggtgtctg gagtccggta tggacagctg cctgtcgaag ccagtaacgc tggatgttat
    19981 aaaacagacg ctgacggtat atgccgagag ggtcaggaaa tcgcgggaat cgtaggacgg
    20041 ataaggcgtt tacgccgcat ctggcattca gtgcaaatgc cagatgcgac gctgacgcgt
    20101 cttatctggc ctactttaat gtaatctgtt gaaataatgg gaatcgtagg acggataagg
    20161 cgtttacgcc gcatccggca atcggtgcaa atgccagata agacactaac gcgtcttatc
    20221 tggcctacag atgattagtc tttatctgcc ggacttaacg ttactgaaga gagataattc
    20281 agcagggcga tatcgttctc gacacccagc ttcatcatcg cagatttctt ctggctactg
    20341 atggttttaa tactgcggtt cagcttttta gcgatctcgg tcaccaggaa gccttccgca
    20401 aacaggcgca gaacttcact ctcttttggc gagagacgtt tgtcaccgta acctccagca
    20461 ctgatttttt ccaacaggcg agaaacgctt tccggggtaa atttctttcc tttttgcagc
    20521 gctgcgaggg ctttcggcag atcggtcggt gcaccttgtt tcagcacgat cccttcgata
    20581 tccagatcca ataccgcact aagaatcgcc gggttgttgt tcatggtcag aacaataatc
    20641 gacaggcttg ggaaatggcg cttgatgtac ttaattaagg taatgccatc gccgtactta
    20701 tcgccaggca tggagagatc ggtaatcaac acatgcgcat ccagtttcgg caggttgttg
    20761 atcagtgctg tagagtcttc aaattcgccg acaacattca cccactcaat ttgctcaagt
    20821 gatttgcgaa taccgaacaa gactatcgga tggtcatcgg caataattac gttcatattg
    20881 ttcatgtaat aggctacctt gctacagcaa gctcttgaca taactgtcaa tgtcgctgat
    20941 gtatttttct attcctggaa catccttctc acgaatcaga tgttccagcg tttcacataa
    21001 ctgcttgccg ggtaccagat ttagcatggc aaatacgcct ttaagacgat gagccgtttg
    21061 ggctaacgca gcaaagtcac tggttgctgc ttcagtatac agcctcttaa catcatccgg
    21121 tactgtgtct acaaagagcg cataatagcc gctggcatgg agttgcgcat tttcatctcc
    21181 gcccagaggc gattctgtca cctcttcctg cgccagttgc acttcaatta attgtaagac
    21241 cgcttcctgc atagcgttgc tcatattgaa gttgacgcac aattgaccag gcccaatttc
    21301 ccgtacgcca gactcatcat cgcttaaaag caagccagag gcagtaagat tagacggatt
    21361 atccgttaaa aagatatcat aatcttgact aattaatctt tcatcgggtg tgatacaggt
    21421 tgcaccccaa ttttctaact ggcgagtgac aatattccga atttctgccg aagtaacatc
    21481 caccattacg cagacatcat ccagtaaacg ctcttcttcc tcttcaactt ccgggtcagc
    21541 tgcgagcatt ttgatatgca cagagtagcg tgtaccaagc ccatcccgcg ttttgatgtt
    21601 taaatgaccg cccagtttac gtgccagttg atcgctcagc cagaatgcca gcgggtccgc
    21661 cttgccatag cgatcgtttt gggtctggtt gataaacggg aagtgcaaat tatccatttc
    21721 atgaatgctt acgccttctc cggtgtccag aatgcggaac gtcaggcggt cttcggagga
    21781 ctcatcctga tcaacctcaa gggtgatttt tcccaattgc gttgaggtca cggcatattg
    21841 catcagtagc agcaaaatac gtcgtaatgc atcgcgatcg ccgcggcgca tatcgtgtgc
    21901 tttcagatga ttgttaatca gcagttgcag acctttacgc ttgatggcag gcaacactga
    21961 aggcacaact tcatcaatta aatcctgcac ggagaacagc accgtctcac ttttccagct
    22021 atcgtccgca agcatgttcg ctaactgtat ttcatcgacc aatcgcacca gcacatctgc
    22081 ctgattcgcc agttgtttgc tttccggggc gttgagttta gccgcgctct ccgccaggga
    22141 ctgtgcgggt tctttcagcg catcgccaat gtttttcata aaggtcatcc gcccctgctg
    22201 gtttttctca tacagacgct gcgcctgctt gagtttcttg tttaccagca cttcgcgatc
    22261 ctgatcgcga ataatgaaaa tttgtgtgcg cggcgcaacc tggctgcgga acatgcggat
    22321 ctcatacagc tcgttattga tcgtcgcctg aataatcccc tgatgctgtt ccgccatggt
    22381 ggtgatgttt tgcagattca aatgcggcag caaatgatcg gcaattttgt tacttatgac
    22441 agtgcggttc gattcctgat cgtgaaccag caggccgagc ggcagcagtg agactatctc
    22501 ttcattgatt gcccgtaaaa tgcgcaattc gttattgacc gccgtgttgg gtagactttc
    22561 tgtactgcga ctggagaaat ggcggaatgt ggtatagcca aataacgcca gcgccagcaa
    22621 accgatgttc agcagcagtg gcagcagaat gttttgcaac gtatccagca ataaggtgcc
    22681 ataaggaacc tgccagacca ggcgcatatc ggtagagttg agtgccgagg agatttcaat
    22741 cttcgtactg ttaaagtgga tactgacgct atccgttcct tctttctcat tatcattgtt
    22801 tcccgttgcc gtcgcgtctg gctcaaggcg gaaactgtcc agcggcatac ccggtgggat
    22861 caaatcatta atcggcagat caaaagccac gaccgttgcc agatgtcccg gctggttgaa
    22921 cgtggtacgc aaggtaaagt aatgaccgtt ctgccaggcc aggcggcgca tgttagaaaa
    22981 actttcgcgt tcatcgaggg cgttggcctg ttgcaacatc tctgcacgac gtgaatcaac
    23041 aatgtcactg acggtcgatt ctttaaatcc ggaggtgaga tctttgaggg gcagggttga
    23101 gatcagcacc agactgttat cctgaccatt caggtaatac attgaccacg gtacattttc
    23161 tgcgccccac aatgtgtcca gataggtgga catccgttga gtcatctcaa gcgttgagtt
    23221 gtcgtgagag ccaaagatta acgcttccgt tttgcggcgc ggtttttcca gatagtagac
    23281 atcctgtttc aggcgcgtct cttgtaaacc ttcgccggag gagggggagg tcgtcgcggc
    23341 aatgttgtcg tagatctgcc aggtcacgta acgccaggta tcgacgcgct tttggatagc
    23401 gtgggtaatg tcgacaattt ggtaactttt atctttcagc caggcgttaa cggcgctttg
    23461 taccattaca cccatcgtca ccagtaacac aatgatcaac agtaaaaaga agcgggtaat
    23521 gctccccggt aggagtgaaa agcgggtcgt ggccgttgtc tctttctgac gcattcgtgt
    23581 ttatgacctg ttaaaacttc gcgaagtgat agggcagtat aaagggtaca gtgtgaaatt
    23641 ccagactctt acctgattga tgattctcta ttctcccgtg atatttatca tcctgcattg
    23701 ggggaaatag atgtacaagc gccattttgc tgtacaaata acctacaaaa agcccaacga
    23761 aaataattgc aaataaacaa agattgctgg aaattatgct gatgttaatt atttgtgaaa
    23821 tagttaacaa gcgttatagt ttttctgtgg tagcacagaa taatgaaaag tgtgtaaaga
    23881 agggtaaaaa aaaccggatg cgaggcatcc ggttgaaata ggggtaaaca gacattcaga
    23941 aatgaatgac ggtaataaat aaagttaatg atgatagcgg tagttattct agttgcgagt
    24001 gaaggttttg ttttgacatt cagtgctgtc aaatacttaa gaataagtta ttgattttaa
    24061 ccttgaatta ttattgcttg atgtt
//
LOCUS       DAZFKY010000062        24025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFKY010000062 DAZFKY010000000
VERSION     DAZFKY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            94..282
                     /locus_tag="ACHKCL_002143"
     CDS             94..282
                     /locus_tag="ACHKCL_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5763108.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            340..1083
                     /locus_tag="ACHKCL_002144"
     CDS             340..1083
                     /locus_tag="ACHKCL_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763109.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHTCNERFSLYSLRNCSRAKAHGAN
                     LSDSCSIFLRRLFRAGDNVLVNSLSVIVLSCIAMRRNTSHHGADSFYPLGSIPRRFSS
                     VRPNSLSQLARLMPSLCASLSNCSFSSGEIRILNCGDCPSPLGLLSRLIVDKWSPIEL
                     AFLLLGGHLNTRALKKAKPRKCHYHSQGF"
     gene            1109..1306
                     /locus_tag="ACHKCL_002145"
     CDS             1109..1306
                     /locus_tag="ACHKCL_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5763110.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGVCHA"
     gene            1299..1484
                     /locus_tag="ACHKCL_002146"
     CDS             1299..1484
                     /locus_tag="ACHKCL_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763111.1"
                     /translation="MLKTFRVFASAVNPLGHTIGIAQNVKAVNVQTAIAAVRSESSEY
                     GLSQVIISAVYELKEVH"
     gene            1484..1675
                     /locus_tag="ACHKCL_002147"
     CDS             1484..1675
                     /locus_tag="ACHKCL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763112.1"
                     /translation="MKEITLHEAAERAHQTEIICRLLEVYPDKLNDGDISALAGLLAK
                     LSGSVALWLIDERAERYEK"
     gene            1665..1907
                     /locus_tag="ACHKCL_002148"
     CDS             1665..1907
                     /locus_tag="ACHKCL_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763113.1"
                     /translation="MKNNKLATSELRSLANSAISLNNAANEVMNMWLQSLVNNERDES
                     TARLILAVSELLDSSTSKIESISEKINGLSGHAIGE"
     gene            1913..2212
                     /locus_tag="ACHKCL_002149"
     CDS             1913..2212
                     /locus_tag="ACHKCL_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5763114.1"
                     /translation="MKLKYPGLTDSGKTRTKFMRGDIYRDQYGGTVMIKGVEERRVTY
                     HREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            2209..4341
                     /locus_tag="ACHKCL_002150"
     CDS             2209..4341
                     /locus_tag="ACHKCL_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO5763115.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGHLAPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHGQKVENWREYLARERQNLSDGLVIEFPVKKKDTGSHSDDELKPRVESRAD
                     GVFWVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKTANHEVITMAI
                     PCGGIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGSHEEWQLSTTTGWHFGAYI
                     MPDGSIIGESEKPILFTGKSAAINGYSVAGTADGWRDSVARLAGGNASMILGVATSLA
                     APLIGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEA
                     HNDGLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEM
                     DVETFLKSEGIKVKAGQLVRLLNVPMEKATKFHEYSNGKEHADALKDAWTANHGAAGR
                     EWVKWLAGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTG
                     WVVQECRDAIQHNFNAWVKEFGTGNREFKQMVEQAEAFLSSFGFSRYLPHPNTDERDL
                     PIKELAGYRKGSIRNEDDEMRYYTFPHVFESEIAKGFNPAHFARALDAAGMLEKGSDR
                     RYKKKALGKIGGKQHVFYVLMFQPDDED"
     gene            4712..4963
                     /locus_tag="ACHKCL_002151"
     CDS             4712..4963
                     /locus_tag="ACHKCL_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763116.1"
                     /translation="MTRTRRDRTEPKYKALDITELALKVAIRTIDRHVGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            4960..5370
                     /locus_tag="ACHKCL_002152"
     CDS             4960..5370
                     /locus_tag="ACHKCL_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5763117.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            5381..5653
                     /locus_tag="ACHKCL_002153"
     CDS             5381..5653
                     /locus_tag="ACHKCL_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5763118.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            5778..6002
                     /locus_tag="ACHKCL_002154"
     CDS             5778..6002
                     /locus_tag="ACHKCL_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763119.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            6295..7452
                     /locus_tag="ACHKCL_002155"
     CDS             6295..7452
                     /locus_tag="ACHKCL_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5763120.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLTDESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKTREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            7492..8064
                     /locus_tag="ACHKCL_002156"
     CDS             7492..8064
                     /locus_tag="ACHKCL_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5763121.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            8066..9277
                     /locus_tag="ACHKCL_002157"
     CDS             8066..9277
                     /locus_tag="ACHKCL_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5763122.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MVRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTHN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            9274..9612
                     /locus_tag="ACHKCL_002158"
     CDS             9274..9612
                     /locus_tag="ACHKCL_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5763123.1"
                     /translation="MNIGRLRDRITIQTLKQTRAMTGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDVARTRL
                     EILCREGAEK"
     gene            9609..9905
                     /locus_tag="ACHKCL_002159"
     CDS             9609..9905
                     /locus_tag="ACHKCL_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5763124.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            9905..10345
                     /locus_tag="ACHKCL_002160"
     CDS             9905..10345
                     /locus_tag="ACHKCL_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5763125.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            10329..10511
                     /locus_tag="ACHKCL_002161"
     CDS             10329..10511
                     /locus_tag="ACHKCL_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763126.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            10635..10991
                     /locus_tag="ACHKCL_002162"
     CDS             10635..10991
                     /locus_tag="ACHKCL_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5763127.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            10975..12636
                     /locus_tag="ACHKCL_002163"
     CDS             10975..12636
                     /locus_tag="ACHKCL_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5763128.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFHIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            12650..12931
                     /locus_tag="ACHKCL_002164"
     CDS             12650..12931
                     /locus_tag="ACHKCL_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763129.1"
                     /translation="MTEAELLGLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMC
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVELHREKEKITYHWKKPERLRR"
     gene            complement(13788..15248)
                     /gene="phoQ"
                     /locus_tag="ACHKCL_002165"
     CDS             complement(13788..15248)
                     /gene="phoQ"
                     /locus_tag="ACHKCL_002165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5763130.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(15248..15919)
                     /gene="phoP"
                     /locus_tag="ACHKCL_002166"
     CDS             complement(15248..15919)
                     /gene="phoP"
                     /locus_tag="ACHKCL_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5763131.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(16088..17458)
                     /gene="purB"
                     /locus_tag="ACHKCL_002167"
     CDS             complement(16088..17458)
                     /gene="purB"
                     /locus_tag="ACHKCL_002167"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5763132.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(17462..18103)
                     /gene="hflD"
                     /locus_tag="ACHKCL_002168"
     CDS             complement(17462..18103)
                     /gene="hflD"
                     /locus_tag="ACHKCL_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5763133.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(18139..19245)
                     /gene="mnmA"
                     /locus_tag="ACHKCL_002169"
                     /gene_synonym="trmU"
     CDS             complement(18139..19245)
                     /gene="mnmA"
                     /locus_tag="ACHKCL_002169"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5763134.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(19299..19760)
                     /gene="nudJ"
                     /locus_tag="ACHKCL_002170"
     CDS             complement(19299..19760)
                     /gene="nudJ"
                     /locus_tag="ACHKCL_002170"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5763135.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(19770..20423)
                     /gene="rluE"
                     /locus_tag="ACHKCL_002171"
     CDS             complement(19770..20423)
                     /gene="rluE"
                     /locus_tag="ACHKCL_002171"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5763136.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            20595..21845
                     /gene="icd"
                     /locus_tag="ACHKCL_002172"
     CDS             20595..21845
                     /gene="icd"
                     /locus_tag="ACHKCL_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5763137.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(21959..23101)
                     /locus_tag="ACHKCL_002173"
     CDS             complement(21959..23101)
                     /locus_tag="ACHKCL_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5763138.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(23091..23327)
                     /locus_tag="ACHKCL_002174"
     CDS             complement(23091..23327)
                     /locus_tag="ACHKCL_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5763139.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            23467..24025
                     /locus_tag="ACHKCL_002175"
                     /pseudo
     CDS             23467..24025
                     /locus_tag="ACHKCL_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         6220 a         5744 c         6414 g         5647 t
ORIGIN      
        1 acatcctcag tatttgccat tcatcactct agcgggaaca atacgacaca ataagacacc
       61 tgatgactct ttaagaaacg aaagggggca atagtgttaa gcactgatcg gtttatacgt
      121 gaaaaagaat gcgaaaaact aaccggcctt agccgtacgt gtcgctaccg cctggaaaag
      181 gccggacaat tcccatcacg tcgtaaactt ggcggtcgtt ccgttggctg gtctttatcc
      241 gaggttctgg cctggaagga tagctgcaag gcagttcatt aatcacgctg gcggcacgca
      301 gccgccacac atcaatcatc tgaacacaga gctataacca tgaagattga atatacgcca
      361 gaacgtgggc ggggattcgt tcgccctggt gaaactgaaa aacaacaaaa ttggggtttt
      421 tcaggtataa aaaaagcggc cccgaaatgg agccgccttt ctgaacagat aacccgctgc
      481 gccgtttgtg tgtgtgatcc caaacataag cacggggatg atagccgcta tcaggctggt
      541 gggcaatgca atcagtctgg ttcagttcgt tgccatacct gcaatgagcg cttttccctg
      601 tactctttaa ggaattgctc aagggcaaaa gcacatggtg cgaatctttc tgattcatgc
      661 tctatctttc tgcgccgtct tttccgtgcc ggtgataatg ttttggtcaa ttctttatcg
      721 gtcattgtgt tgtcctgcat agcaatgcgc cgtaatacct cacaccacgg cgctgatagt
      781 ttttatcctt tgggttctat accgcgccgc tttagttcag tacgccccaa ctctttaagc
      841 cagttggcta ggcttatgcc gtcgctctgt gcttctttat cgaattgctc ttttagctct
      901 ggagaaattc gcattctgaa ttgtggggat tgcccgtccc ctttagggct tttatcgcgt
      961 ttgattgttg acaagtggtc acctattgaa ttagcctttc tattgttagg tggtcacctt
     1021 aacacaagag cacttaaaaa agcaaagccc cgcaagtgtc attaccactc gcagggcttc
     1081 taaccaacaa cgtaaactag gagccgttat ggttgctgta aatcatatac cacaccttgt
     1141 acacacacaa acggcctttg tgtggcgttt tctggcactg agtgccggag aatctcaaat
     1201 catccacgta accgcctgga cggaacgcga agcgcgtagc cgttgcccgt caggttgtgt
     1261 tgctgtattt gccgcccgta ttcgtcaggg ggtgtgccat gcttaaaacc ttccgtgtat
     1321 ttgccagcgc tgttaaccca ctaggccaca caattggtat cgctcagaat gtgaaggctg
     1381 ttaatgttca gacggctatt gctgcggtga gaagcgaatc atcagaatat ggcttatcac
     1441 aagtcattat ttcagcagtg tatgaattaa aagaggtgca ttaatgaagg aaattacatt
     1501 acacgaagcc gctgaacgtg cgcaccagac agaaattatt tgccgccttc ttgaggtata
     1561 ccctgataaa ttaaatgatg gtgatatatc cgcattagct ggccttctgg caaaactgtc
     1621 aggaagtgtt gcgctgtggt taattgatga gagagccgaa cgctatgaaa aataataaac
     1681 tggcgaccag tgaattaaga agccttgcca acagcgcaat atctttaaac aacgcagcaa
     1741 acgaagtaat gaatatgtgg cttcagtcgc tggtgaataa tgaacgcgat gaatcaactg
     1801 caagattaat attagccgtt tctgaattac tggatagttc aaccagcaaa atagagagca
     1861 tatcagagaa gattaacggc ttatcaggtc acgctatcgg ggaataaaaa taatgaaact
     1921 taaatatcct ggcttaactg acagcggcaa aactcgcact aaattcatgc gcggggatat
     1981 ttaccgcgac cagtacggcg gcacggtaat gattaagggc gtggaggaac ggcgcgtaac
     2041 ctaccatcgt gaaggctacg aatatgattg cgtgatgcct gtttatcagt tccggcgtga
     2101 tttttctctg gtacaggcag cgccccgcag taaaccaacc agcagggaga aagcacgcgc
     2161 caatattcag gaaataaaaa agatgcttaa cgtattcagg ggtaaaaaat gaaactggca
     2221 ccgaacgtaa aaaaacagcc acgcggaata aaacacaaag acacagaggt gattattttc
     2281 gcgggtagtg atgcgtgggc acatgcaaaa cagtggcagg agcaggacgg ccccgcatcc
     2341 ggcgataatg tgcctcctgt gtggcttggg gagcagcagt tatcagaact ggataagctg
     2401 caaattgtgc cggaaggcag aaaatccgtg cgcatattca gggccggaca tcttgcacca
     2461 gtaatgatca aggcgattgg tcaaaagctg gcggcggctg gtgtacagga tgcaaatttt
     2521 tatcctgagg gtatgcacgg tcagaaggtg gagaactggc gcgaatatct ggcccgtgag
     2581 cggcagaatc tttctgatgg tctggttatt gagtttccgg taaagaaaaa agataccgga
     2641 agccatagcg atgacgaatt aaagccacgc gttgaaagtc gcgccgatgg tgttttctgg
     2701 gtaacgccca aagtggataa gcagtcaggc gaaattatcc ggcctgagac gtggttatgt
     2761 tctccgcttg aactactggg aacggggacg atcggtaaag agcattaccg cgtgatgcgc
     2821 tggaaaaaaa ccgcaaacca tgaagtcatc acaatggcga tcccgtgtgg tggtatcggc
     2881 gaccgtgacg gctggcggtt acttaaagat cacgggctga acgtgacaac caacggcaaa
     2941 tacagggcaa tcctggcgga ctggatgcag ttaagcggaa gccatgagga atggcagtta
     3001 agcacaacaa cgggctggca ttttggcgcg tacatcatgc cggacggttc gatcattggt
     3061 gaatctgaaa aaccgatcct gtttaccgga aaaagcgccg cgattaacgg ttattccgtg
     3121 gcgggcacgg cggatggctg gcgcgacagc gtggcgcggc tggctggcgg taatgcctca
     3181 atgatactgg gtgtggcaac gtcactggca gcgcctttaa ttggccttgt gggtgctgac
     3241 ggcttcgggg tgcatctttt cgaacagtca tcggcaggta agaccaccac gcagaacatc
     3301 gcatcaagtt tatggggaga gccggacgcg cagcggctga cctggtacgg cacggcgtta
     3361 ggtattgcca atgaggccga ggcccataat gacgggcttt taccactgga tgagataggc
     3421 caggccggaa atgcgcggga agtgtcaacg tctgcttata cgctgtttaa cggttcaggg
     3481 aaattgcagg gggcgaagga tggcggcaac agggagatca aacactggcg aacggtggca
     3541 atcagcaccg gagaaatgga cgttgagaca ttcctcaaaa gtgaggggat aaaagttaag
     3601 gcggggcagc ttgtccgact gcttaacgtt ccgatggaaa aagccacgaa atttcacgaa
     3661 tacagcaacg gtaaggaaca tgctgacgcg ttaaaggatg cctggacagc aaatcacggg
     3721 gcggcgggtc gtgagtgggt taaatggctg gcaggccacc agcaggaggc aaaggacacg
     3781 gtaagggaat gccgcgaacg gtggcgcaac ctgataccgg agagttacgg cgagcaggtc
     3841 caccgcgtgg gtgaacgttt cgccatactg gaggccgcgc ttgtgctttc cggtcatgta
     3901 actggctggg tcgtgcagga atgccgcgac gcgatacagc ataactttaa tgcctgggtg
     3961 aaggagttcg gcacgggtaa cagggaattt aaacagatgg ttgaacaggc tgaggcgttt
     4021 ttgtcgtcgt ttgggtttag ccgctacctt ccgcacccca atacagatga gcgtgattta
     4081 ccgattaaag agcttgccgg atacagaaaa gggagcattc gcaacgaaga tgacgagatg
     4141 cgctattaca cgttcccgca cgtgtttgag agtgagatag cgaagggatt taatccggcg
     4201 cattttgcgc gtgccctgga tgctgccgga atgctggaga agggaagcga tcgccgatac
     4261 aaaaaaaagg cgctgggcaa gattgggggt aagcagcatg ttttttacgt gctgatgttc
     4321 cagcctgatg atgaagatta atcccctgtg tgaggaaaaa aattgcgggt tatgcgggtt
     4381 atcctgtgta taagtgtatt aagtgtatga atataaagga aatcaataac ccgcacgtaa
     4441 cccgcaaaag ggcatttata acccgcaaaa gcacgattat aacccgcagg cgaataactg
     4501 aagtaacacc ggacagtcat gcgtaacccg cagaaaaaac cccgtttgcg ggttattttg
     4561 agcgttttgc gggttacgtg ttgagcaaat aataaacagg gtgttttgca acatgctgat
     4621 ataaaagggt aaaaaatggt tagcacgcta agataaccca ataacccgca taacccgcac
     4681 atgtttctat atatataaat tgaggtggca aatgaccaga acacgcagag acagaacaga
     4741 gccgaaatat aaagcgttag atataacaga gctggcctta aaggtggcaa tcagaacgat
     4801 agaccgacac gtaggggaag gatacgcgaa ggaacatccc gacctgataa gcgcattcat
     4861 gaccacagca gcggcaaact ttgccacgtt gacagaacgg gagattgccg aagcggaaca
     4921 ggtaacaacc atcaacgtta aaaccggaga ggtggaatca tgacagcaca gatagcggct
     4981 tacgggcggc tggtggacga cccgcaggta aaacagacca gtaagggcac accgatgacg
     5041 ctggcgcgta tggcggtatc gttgccatgt agtcaggcgc aggatgggca ggcgacgtta
     5101 tggctatcgg tcatcgcatt tggtaagcag gccgacttcc tggctaaaca tcaaaaaggc
     5161 gacgttgcca gcgtatccgg cacgatgcag gtcagccagt ggaccggaca gaacggggaa
     5221 acgcggcagg gttatcaggt tattgcagac agcgtaatca gtgcccgtgc ggcacgtcct
     5281 ggcgggaaca gacgcaaaac cacaggcaca cagggtaatc agccaccagc gggaggcgat
     5341 gacccttacg gtgacggtat tccgttctga gggggtggcg atggtacatg accgcatagc
     5401 ggaagaactc gaggcgaaag gcttttaccg gagggcggcg gcgcgatggg gtgaagtcat
     5461 gctgctggtg gagacagaca aggaacggca tcaggttacg atgcgacggc tggaatgttc
     5521 caggaaggca cagaagccac cggagccgcc ggataacttc ggagacctga gaaaggcagt
     5581 cgatcgcact tatgctgaaa tgggtataga tggtgtaagc gatgaaatat ggcgtaatta
     5641 cccagacagc taatcaacag ccggagaaat ccggcttttt ttgcaccagt tgaaacggta
     5701 tggcgcatta ccgggttttc gtcacggtca ggcatagtta ctatctgaaa caaacagaca
     5761 caacagagga aaaaacaatg ccgatgaaat ttgatgagat attaaaacag cgtgataaat
     5821 accatgctga caacatggag acgatgagca tcaatgatta ccgcgcattc ctggagacgg
     5881 gcgcactgat tgaaaaggat cagcatggtt ttgtgagatg tgctctgtcc ggtgaaatgc
     5941 tggcggtaaa tcctgaacag atagatgcat tgatagaatt tctgaaaggg atcagggact
     6001 gagcacgcat acagccggag caatccggct ttttgtcatt ttttgtaaat tatttgttcg
     6061 tggttgttcc acgttgttca ctgacaggat cggcatattt tacccgaact gaatcatgag
     6121 tattctcgcc cgtggtgcca ggacgctggg gccactttcc cgcctgttaa tgtgctcgcc
     6181 aatattcatt accaggcggg aaaacgatcg gtgcgattgc tgatttcctt atgaaaaacg
     6241 gttgagtttt tgccgcgtcc tggagttcct tacttaaccc caggactttt ttttatgccg
     6301 agaataatcg aattacgcca gcagaaaacc gccattaaaa atcagatgcg cgacatgctg
     6361 gagaacgcgg aaaaagaaaa ccgcagcctt aacgatgcag agggcgcaaa atttgacgaa
     6421 ttacgcgcta aagctgaatc cctcgataaa gacatttccc gccttgaagc cattgcagac
     6481 gaagagcgca gcaagccagg taaaagcagc cagaccactg acccggcaga actccgcaac
     6541 tacatcctga caggtgaaac ccgcgcatta agtacaggcg ttcccgctga tggtggttat
     6601 accgttatcc ccgaactgaa caccgaaatc atgcgaatgc tgacggatga atccaccatg
     6661 cgccgcatct gtaccgtgaa gaaaatcagc agcaacgagt ttaagcagct tgtttccgct
     6721 ggcggtgcga ccgttaacca cggtgaagag ggtaagacac gcgaacagac cagcaccccg
     6781 cagattaacg aggtgagcat taagctgtat ccggtctatg cgtacccgcg caccacacag
     6841 gaaatcgtgg atttttccga tgtggacatc ctttcatggc tgacgggtga gattggcgac
     6901 accttcacgg aaaccgaaga aagcgatctg gttgtgggcg acggtgacaa aaaagcaaaa
     6961 ggttttttat ccgtaccccg tgcagagaag aacgacaaag agcgtgattt tggtacgttg
     7021 caggtaatta aaccttccga atctctggcg tggacatctg cggacccgct gatcgacctg
     7081 aaatttgcat tacgtaaaaa ataccgcaaa aacgcggtct gggtggttaa ctccacgacg
     7141 gcggcaaaac ttcagaaggt gaagaacgcg aacggtgatt acatctggcg cgaccgttta
     7201 caggcgggtg atcctgatac gttgctgggc cttccggtcg aatatctgga gtttatgcct
     7261 gataacgtta ttgccctggg tgacttcaaa cgcggttact acattgttga tcacgaaaca
     7321 ggtgttcgca ccagaccgga caacctcaca gagccgggtt tcatcaaaat tttcacgcag
     7381 aaatatttag gcggtggcgt ggtggattcg aacgcgatca agattctgga actgccacag
     7441 gacgacgatt aacagcatac agaaggggct taaaagcccc tttagtgttt tatgggtgaa
     7501 aaaattatga agagtatgga aatccggtca tcggaaatca ccaccagcgc cagcaacacg
     7561 cttacaggct acgttgttcg ctgggataac ctttcagagc tgctatgggg ggagttttac
     7621 gaaaaattcc agcggggagc gtttactgaa tggcttgcgg cgggtaatga cgttcgcggc
     7681 ctgtatgagc atgaccacag catgttactg gggcgcaccc gttccggcac gctgaaactg
     7741 gaagaggacg aaacagggtt acgctttgaa ctgaccccac cggataccag tacagggcgc
     7801 gacgttatcg aactggttaa gcgtggggac atctcgggga tgagctttgg ctttcgctcc
     7861 cgtaaggatg tatgggatac cacaacagat ccatgcgtgc gcaccgtgct ggtggcggaa
     7921 ctgtacgaaa ttaccgttac atcggtaccg gcttaccccg attccggcgt ggagctggcc
     7981 cgccgttccc tgtatgagca gcaccccgaa aaaatgccgc gtgcggataa tcgccgctgg
     8041 tgggcggatt tagcgggggt gtgatatgtg gcctttcaga agaaaaaaag agcagcgcag
     8101 catgacgctt gatgagttta tggcgctggc tggcacatcg aacacggggg cgggtgagta
     8161 cgtatcatcg gggacagcgg aatcactgcc cgccgtcatg aacgccgtga cggtcatctc
     8221 tgaggcggtg gctaccatgc cgtgttacct gtacctggta cgcaatgaga aggggaagga
     8281 ggcccgcgag tggcttgatt ctcatccggt cgatcatatc ctcaacgagc gcccgaacgc
     8341 gtggcagacg ccctatcagt ttaagcgaat gatggtccgc cactgcctgt taaacggtaa
     8401 tgcttatgcg gtgattcagt gggggcgtga tggttttccg gtggctttgc acccttaccc
     8461 gccgcagtcg gtgaacgtgg agcagacagg cgaacacaac tggcgctatt gcatcactga
     8521 cgcctacacc ggaaacaccc acaactattt accgtgggaa gtacttcacc ttcgttactc
     8581 cacggatgac ggctttatgg ggcgctcacc tgtaaccatc tgccgcgaat cgctggggct
     8641 tgggctggcc caacaacgcc acggcgcgag cgtgatgcgt gatggcatga tggcggcagg
     8701 ggttatcacg tcaggcgaat ggctggacgg cgtgaagggc aaacaggcat tagccgcact
     8761 ggaacgctac aaaggggcca gaaacgccgg aaaaacgccc atccttgaag ggggtatgag
     8821 ctatcagcag ctgggcatga gtaatcagga tgctgaatgg ctggcctccc gtcgctttac
     8881 cattgaagac atcgcccgaa tgttcaacgt ctcgccgatt tttttgcagg aatacagcaa
     8941 cagcacctac agcaatttca gcgaggcaag ccgcgcattt ctcaccatga cgatgcgccc
     9001 gtggctggcg aactttgagc agcagataaa aaacgccctg ctggtggcct cgcctgtacc
     9061 tggtatccgg tatcaggtgg agtttgacag cgcggacctg ttacgggcca cacctggcga
     9121 acgctttgcc acctatgagc gcggcatcaa atccggcgtt atgtgcccga acgaagcccg
     9181 cgaacgtgaa ggactgtcac cgcgtgatgg tggtgatgag ttcagccagg catggaaaca
     9241 ggaagtaaaa atcagcgagg gagaaaaacc ggaatgaaca tagggcgact gcgtgacagg
     9301 ataacgattc agaccctgaa acagaccaga gccatgacgg gcgaaatact cgaaacgtgg
     9361 gaggacggtc acacactctg ggcaagcgtg aacatgatca gcagcaagga ggccatttca
     9421 tcgggcgcag agctggcgat tggtaccgta aggatctgga tacggtaccg gaaggacatc
     9481 aacgccacca gccggataaa ggtcagtacg gggccgctgg cggggcgtgt actgaatatc
     9541 atcgggcagc cgttgccgga tgtcgccagg acacgccttg aaattctttg tcgtgagggc
     9601 gcggaaaaat gacagaagaa cttatcaccc tggaagaagt gaaactccat tgccgcatcg
     9661 atggcgacga ggaagaccag ttaatcagcg gatacattgc cgcatcgctt gaggcgtgcc
     9721 agatacacat aggcaggcgc tttgatgacg ggctggagtt cacgccagcc ataaagattg
     9781 gctgcatgat gtttatcgct cacctgtatg aaaatcgcca gattgtcgcg gataacgcaa
     9841 aaacacgcgt acccatgacg attggtgcac tctggacggc ttaccgtgat gtgggggtgt
     9901 actgatgccg tggcaaccat taagacgatg cacagaacca ggctgtaaca ggcgcgtgaa
     9961 gtccggcaag tgtgaggagc acaggcgggc ggcatggcgt gcagaggatg ccagacgagg
    10021 acaccgccgc gcgcgtgggt actccagaca gtgggacaaa taccgcgcca tgtatctgag
    10081 taaaaacccg ttatgcgtgc gatgccttga gaaggggata tatacgcccg ccgtggtggt
    10141 ggatcacatt atccccatcg atggcggtga tgatgttctc ttctggcccg aatggaacca
    10201 tcaaccattg tgtcaggcgt gccataacca gaaaacgaaa tggcttgatc cggcaacaaa
    10261 aagcaagcgt gccgcaggtg gatttcgtga agaggaagaa cgggccgcta accgcaataa
    10321 ctggatgtat gacgctgatg aatgagcggg aacaaaaccg ccttatccgt ggacttataa
    10381 ggcagcgtga cgcatggaag acacaggaaa cagggcataa agataaagcg tcaggacgcg
    10441 cagaacgcat cacagcgaag cgattaaccg accgtgaccg cgaggttatg gaatgtttcc
    10501 gcaatcgctg atgaggctgt atgacggggt ggggggcgtt ttcaggacaa acccgacccc
    10561 gccgggcacc gaacgcctcc tcaaattttt atgcacggga attttttgaa aaataatctg
    10621 gcgaaaaaat aagcatggca agaccaccga aagcccccgc ctacctggat gatatcgccg
    10681 tgaagcagtg gcgggaaaaa tcgcggcagc ttgcggaacg gggtgacctg actcccgccg
    10741 actggagtaa tctggaactg tattgcgtca attactcgat ataccggaaa gccgtcgcag
    10801 accttgcggc gcgcgggttc agcattgtta acagtcaggg cggagagagc agaaaccccg
    10861 cattaagcgc gaaatccgac gcggaaagag tgatgataaa aatggcctcc ttgctcggtt
    10921 ttgacccgat aagccgccgt aaaaatccac cggaaacaga agaagaggac gagcttgacc
    10981 gcctggaata agtacgcaga agacgtaaaa acgggcaaaa ttccggcctg taaacggtta
    11041 aaacaggctg ttaaacggta cttttcggac cttaaaagcc ccctttacac gttcgatcgt
    11101 gaggttgtgg agcggtttat tgccttttcc agggtgtgcc cgcacgtaaa aggaccgatg
    11161 cgtggcagac ccattgagct ggagccgtgg cagcagttcg cctttgcgtg catcctcggc
    11221 tttaaggtta aggccaccgg acggcgcaaa tacacgagcg cctttatcga agtgccgcgc
    11281 aaaaatgcga aatccacgac cgccgcgatt ctggctaact ggtttttgat tatggagaac
    11341 ggtcagcagg atatctacac cgcagcggtg agccgtgacc aggcgcggat cgtctttgat
    11401 gatgcgcgtc agatgtgcct tttatcccga ccgttacgca ggcgggtgaa tattcaggcg
    11461 cataaggtga tacacccgaa aagcaacagc ctgttaaagc cgctggcagc aaaagcggca
    11521 accattgaag gtacaaaccc gagtcttgcc attgtggatg aatatcacct gcaccctgac
    11581 aacggggttt attccgcgct tgaactggga atgggggcgc gtccggaggg gctgttattt
    11641 gccatcacca catcggggag caacgttgtt tcagcctgta aacaacacta cgactattgc
    11701 tgccagatac tggatggtga agaggtgaac gaatccatgt tcgtgctgat ttacgagctg
    11761 gatgatgaaa gcgaggttga cgatccggcg atgtggataa aggcgaatcc caatatcgat
    11821 gtttccgtcg atcgtgaaaa actggcctca accatccaga aagcgcgggg aataccgtcg
    11881 cagtgggtgg agatgatgac aaagcgattt aatatctggt gccagggggc taccccgtgg
    11941 atgggtaacg gcgcatgggc tgagtgtgcc ggaacgttca cggaggaaga tttacacggc
    12001 caggagtgtt acgcggggct ggatttatca tcaaccagcg atatttccag cgtatgctat
    12061 gcctttccgg tcggtaaaac cattatgctg atttcccgtc attatctgcc ggagttccag
    12121 ctacagaacc ccgccaataa aaaccgcgct gtctatcgtc agtgggctaa agcgggctgg
    12181 atacgcacaa cgcctggcga ctgcattgat tacgaccgga tcagagatga catcatgcag
    12241 gacgcggaga aatttcatat caggctggtg ggctttgata cgtggaacgc cacgcatctc
    12301 agaacgcaat tacagggggc aggttttgag gtggagccgt tcccgcaaac ctacctcaga
    12361 ttcagtccgg cggcgaaatc gttcgaagtt tttgttaacc gcagggtgat tgtgcatcgt
    12421 ggcgatccgg tgttgtcctg gtcgatgagt aacgtcgtga tgcagagtga cgcgaacgcc
    12481 aatatcaagc cgaacaagaa aaaatcaccg aacaagatag acccgagcgt ggcggcactg
    12541 atggcgtttg gtacattcca ggcagagcat gaggattttg cttttgatat aagcgacagc
    12601 caccgccaga aactggaaga attcagcggg gtgtaatgag gtcagcagca tgacagaagc
    12661 cgaactactg ggattaatcc gccgcgtatc tggaatcagc cagcaggctg acgaacagac
    12721 cacgcagccg gacagcgtga cagccgaaaa ttatgcgcgt gttgttgctg aggtgatgtg
    12781 ccgtgatggt atccagctta atgatgttga tatgcgcaac atacggatcc gcgttcttga
    12841 aatgctggcc tacaatcgcc gcgttgaact gcatcgggag aaagaaaaaa taacgtacca
    12901 ctggaagaag ccggagcggt tgcggcggta actggttgat attttcgaaa ttcgccagcg
    12961 gcgaacatcg gggaaaccag cctaacccgt tgattctttc ggaaacagcc attggcggaa
    13021 tccggcgcgg taacttgctg atattcccga taacgcaaaa ttgcgtggct gaatgaacat
    13081 tatgattact tatgattacg cagatgatta aggcctaaac cgttgattct tcccaatttc
    13141 cacagactgt ggaagttggc gcggtaactt actgagatta aagcaaagcg caaaattgcg
    13201 ccgactacat ccctcacatg cgaaattttc gcagtttaat tactcgccag tttgatcgtt
    13261 aacccgctga tattttcggg aacctcaatt tgaggaagtc ggcgcggtaa ctggttgata
    13321 ctttcgacct cctcaatttg aggaggtcgg ggcacagaaa aacaacgaat atgttgtttt
    13381 tgggaatggt cataatgacc acgcagacca tcaaggcaac cacaacgaac atcgttacgg
    13441 ttggagcttt taccccactg ggggaaaggt attacgataa tcataacacc taccgaacag
    13501 gtaaaaccca ccagcctgat taacagttaa ccggaaaaaa aatccggtag ccccgttatg
    13561 gggatcccca tatcgacatt aacgcccctc atgaattggt gcgcttcccc ctggaaagat
    13621 tacccgcctg tatatttctt gtgtctattt gttccacgtt gtttcataca gtgcaccgaa
    13681 cggtgtagtt aatggtgtag tcaatttgct gtttttagca ctttttgcag tgtgggttgt
    13741 ttaattaaat caatgggttg ccttgctgtg gtgataaaag gacatattta ttcatctttc
    13801 ggcgtagaat gctggcgacc aaaaatcacc tccatccgcg caccgccaag catgctctct
    13861 ccggcgacga ttttaccctc atattgcttg gtgatttcgc gggctaccgc cagccccaca
    13921 ccttgcccag ggcgtaaagt atcaacccgt tgaccacggt cgaaaatgac ctctcgcttg
    13981 cttaatggaa taccggggcc atcatcctcg accacaatat agagatgctc gtcggtttgc
    14041 cttgcagaaa tttcgacaaa ctcgaggcaa tatttacagg cattatccag tacattgccc
    14101 atcacctcga caaaatcgtt ctgctcaccg acaaagctga tctctggcga aatatcgaga
    14161 gagatattga cccctttgcg ttgatacact ttgttcagcg ccgaggtgag attgtccagc
    14221 agtggggcga ccggatgcag ctcgcggctg agcaatgtcc cgccgtgcat actggcacga
    14281 tgcaggtagt agccaatttg ctgtgaaatg cggctgattt gctccagcat taccggttca
    14341 gcatcactga cgctcatctt ttcactacgt agagaacgca gcgtactttg cagcaccgcc
    14401 agtggcgttt tcagactatg ggtcaggtcg gtgagcgttg tacgatattt gtcgtaacgt
    14461 tcgcgttcac tttttaacaa tcggttcagg tttcgtacca gactggtcag ttctcgcgtt
    14521 gtggctggat tgagcaattc gcggttatgt tcttccagtt cgcggacttc ttttgccagg
    14581 gcttcgatgg ggcgtaaact ccaccaggcg gcgacccaca gcagcgggat cactaacagc
    14641 agattggctg agagcacata gataaaccag ctccagacca tataggaact ttttagctcc
    14701 accggaatgg tatccaccac cacaatggtt aactttggca tccgcgatgt tgccgggtag
    14761 acgtttaccg ccaccgagtg ggtcatctcc gcgtcgtcat catcttcccg cacttcctgc
    14821 aactgttgct gtatcgaatg atctccactc agcaagaggc tggtatcgtt aacatccgct
    14881 tcaatttcat gaaaaccatt cgatttcagc cagtcaggct ggatcatctt catcagccag
    14941 ggcacgtcac gttgcgccca taaaagctgc ccgttctcat cataaattag cgtcatggtg
    15001 gggctttgct tgtcgatatt ttcgggtaac tcgacatgca acttattgtt ttcccacttc
    15061 gcaagggtat agaacagatt gctctcgcca cgtaacagcc gaaacgtagt tttatcgaaa
    15121 ctgacgctat aaccgatcag cgcgaccatt ccgtaggcaa gcgaaagcac cagtactacc
    15181 gctgccgttg ccaacagaaa acgtacccgc agcgagagcg ggaaaaaaag acgcagtaat
    15241 tttttcatca gcgcaattcg aacaaatagc cctggccgcg aacggtagta atcacttctt
    15301 gcggatattg tgcctgaatt tttttgcgca gacgtcccat cagtacatca atggtatggc
    15361 tttcccgcag ctccgcatcc ggatagagtt gtagcattaa cgaatctttg ctgaccactt
    15421 tgccattatt gcgtatcaac gtttccataa tggtgtattc gaacgcggtc agtttgatca
    15481 cttcgtcatt aatagataat tcacggcgag agagatcaac ctgaaacggg gggagcgaaa
    15541 tgacctgtga agccaggccg ctattacgcc gcattaatgc ctgcattcgc gccatcacct
    15601 cttcaatatg aaacggttta gtcacgtaat catcagcacc ggcacttaat acttcgactt
    15661 tgtcctgcca gctttcacgg gcggttaata ccagaatcgg cagtgaaaca tcattgctac
    15721 gccagcggca aatcagtgac agaccgtcct cgtctggcaa tccgagatcg acaatcgcaa
    15781 tatccggtaa atgttcattg agataataat cggcttcttt ggcatcttct gcatcatcga
    15841 cctgatgacc agcatcctga atctgaactt taaggtggtg acgtaacaac gcattgtctt
    15901 caacaaccag tacgcgcatt tttatttctc cctgtcttaa ttattaaaat agtgtaacgc
    15961 gattatgtgg ttatgggggt aaacattaaa taaaccagcg ggggagggga ggtaaagtga
    16021 aaaaataaaa agcggataat cttaataagc aggccggaca gcatcgccat ccggcactgg
    16081 tacgagttta tttcagctca tcaaccatgg tgatggcgcg accaatgtag ttagccggcg
    16141 tcatcgcttt caggcgggct ttctcttctt ctggcaacgc cagaccgtcg ataaactgct
    16201 tcatgccttc ggcgtcaacg cgcttaccgc gagtcagctc tttcagcttc tcgtacggtt
    16261 tttcgatgcc atagcgacgc ataactgtct ggattggttc tgccagcact tcccagttgt
    16321 gatccagctc atccagcaga tggtcacggt tcacttccag tttgctcacg cctttcaggg
    16381 tggattgata cgcaatcagc gcataaccga tacccacgcc gaggttacgc agcacggtgg
    16441 agtcggtcag gtcacgctgc cagcgggaaa ccggcagttt gcttgccaga tgctgcaata
    16501 ccgcgttgga aaggcccaga ttcccttcgg agttttcgaa gtcgatcggg ttaactttat
    16561 gcggcatggt ggaagaacca atctcaccag caatggtttt ctgtttgaag tggttaaggg
    16621 cgatataacc ccagacgtca cggtcaaagt cgatcagaat ggtgttgaag cgcgcaacgc
    16681 aatcaaacag ttcggcaatg tagtcgtgcg gttcaatctg ggtagtgtac gggttccatt
    16741 gaatacccag cgaggtgacg aactcttcgc tgaactgatg ccagtcaact tccgggtaag
    16801 cggcgatgtg ggcgttatag ttaccgaccg cgccgttgat tttgccgagg atctccacct
    16861 ggttaagctg gcggtactgg cgctccatac ggtaggcgac gtttgccatc tctttaccga
    16921 tggttgacgg cgtggctggc tgaccgtggg tacgggacag cagcgggata tcgcgatact
    16981 gaacggcgag atctttaatg ccatcaatta gttgacgcca gtacggcagg atcacttcat
    17041 cacgcgcggt tttcagcatt aatgcgtggg agaggttatt gatatcttcc gaagtacagg
    17101 caaagtggat gaattcagaa accgcgtgca gttccgggat ctccgccact ttttctttca
    17161 ggaaatactc aaccgcttta acgtcgtggt tagtagtacg ctcgatggtt ttgatgcgtg
    17221 cggcatcttc ttcgctgaaa ttggcgacaa ttgcatcaag gtaaccgatt gcgtcggcag
    17281 caaaagcagg aacttccttg atcgctgcgt gcgcggccag tttttgcagc caacgtactt
    17341 caacttgtac acggaatttc agcaaaccat attcgctgaa aatcccgcgc agcgcgctga
    17401 ctttatcgcc gtagcgtcca tcgacagggg aaacggcggt cagtgaggat aattccatag
    17461 atcacaactc cggggttaaa tgagcaagaa tttgttttgc ctgagtggtc aggcgattac
    17521 gagaaaacat cagttgcaga cgtccgccgc cgacctggtg ccagagcacg gcggcgcgaa
    17581 tgcctgccag cagggttgcg cgaactttcg cctgcacttg tgggctttgc agtacagcag
    17641 gggaaccggt gacctgaatg cgcgggccaa gcgggctgat cacatcaaca tagatagcag
    17701 ccatcgcgct catcagcgtt tcggactgta aatcgaagtg ttcgagctgg cgttgcaggc
    17761 cgttgatccg gttgcccaga gtgtcgagcg cacctttcgc tgaggagagt ttgcgcttaa
    17821 gcaccatcag gctgagtgtg tagcgggtta attcggcgtt taagccctgg cgactgctgg
    17881 cattgagcac gccgagcagg gtttccagcc cgacgcgcag gttggcttcg ctaccgccaa
    17941 aaaccgccag cgtcgagctg gggttcatat caataatact gttgagtgag acgtgtagcg
    18001 catcggcatc acaatgcccc tggtgagcga gttgttgcac caggcgtgcc gactgacaaa
    18061 taccggccag ggcgagggtg atgtcatagt aattctttgc cacgttcact gcttccttgt
    18121 tttaagtaaa gataataatc agatcggcag cggcagacgc tgctcaataa taccgccacc
    18181 gaggcacact tcaccgttat agaagacggc agactggccc ggcgtcacgg cggcaacggg
    18241 ctcatcgaaa atcacttcaa tgcgatcatc gtccagcgcc ttgacggtgc aggggatatc
    18301 ggtctggcga tagcgggttt ttaccgtgca acgcatagta ccggtgaatg gttcacgatc
    18361 gacccagtgc agctgctggg caatcagacc gacagacatc agacgcgggt gttcatggcc
    18421 ctgcgcaaca atcagaatgt tgttttcgac gtctttatcg accacatacc acggttcttc
    18481 ggtgccttct ttggtgccac cgatacccag acctttgcgc tgaccgaggg tgtgatacat
    18541 cagcccctga tgctcaccaa tttcatcgcc atcgactgta atgattttgc ccggttgcgc
    18601 cgggagataa cggccaagga actcgcggaa tttacgttcg ccaatgaagc aaatgccggt
    18661 agagtctttt ttcttcgcgg tgaccagacc aagatcttca gcaatcttac gcacctgcgg
    18721 tttttccagt tcgccgaccg ggaacaggct ttgcgcaatc tgctcatggc tgagcgtata
    18781 aaggaagtag ctctggtctt tattgctgtc cagaccacgc agcaggcggc tcttgccatc
    18841 gacatctgcc cgacgaacgt aatgaccggt agcgatataa tcggcaccta aatcttcggc
    18901 ggcaaattcg aggaaggctt taaatttgat ctctttgttg cacagaatat ccggattcgg
    18961 cgtgcgaccg gctttatatt cggcaaggaa cagttcgaag acgttgtccc agtactcggc
    19021 agcaaagtta acggtgtgca gttcaatgcc gagcttgtcg cagacagcct gggcatcagc
    19081 cagatccgct gccgctgtgc aatattcctc accgtcgtct tcttcccagt tcttcataaa
    19141 caggccttcg acctgatatc cctgttgttg caacagccag gcagaaacgg aggaatcgac
    19201 accgccggac atgccgacga ttactttttt tgcggtttca gacattggat cactcacgac
    19261 attgaacttc aaggcggcgt attctatcac gcagcctttt agatgacacc ctttgtaaaa
    19321 ggccagttaa aatcgccaat catctccagc ggatagcgtt gcccgctttg ataacaacgg
    19381 atactttccg ccaccagcgg cgaacgaaga tttgacgcct gtaaaatttc ttcggcgctg
    19441 acccaacgac agcagtcgat atcgctgtca tgaggctggg tcgggcatat ttgctcaagc
    19501 tcaatggcaa agaggaaacg caaaaacggc gttttatctg gcgcaatcca ctgatgcata
    19561 cgaataaagt gttgcggctg cgcgctgatg ccggtttctt cccacagctc acgggcggct
    19621 gcttccacta aggtttcatc ggcttccaga tgcccggcag gttggttcca taaggcttta
    19681 ccattaatcg tctcttcaac gactaaaaat ttcccctctg cgtgcactac gcaagcaacg
    19741 gtaacgtgcg gtttaaacat tgtgcatcct tattctgttg cttctcgcca ttcgccattg
    19801 gcaaggttat ccaaagagta atcacccatc gcatagcgaa tcagtcgcag cgtggggaaa
    19861 ccaacatggg cggtcatgcg gcgcacctgg cgattacgtc cttcatataa ggtgatcttc
    19921 agccagctgg tgggaatact tttgcgttca cgaattggtg gattccgcgg ccataaccac
    19981 gcgggttcgt caaccagctc cgcgcctgcg ggcagggtag ggccatcatt taaggttacg
    20041 ccattgcgca aggcttcaag tgcgtcttgt gtgggaatac cttccacctg cacataatag
    20101 atttttccgg tgcgtttgcc cggctgggtt aaacgcgcct gcaacgcgcc gttattggtc
    20161 agcaccagta acccttcgct atcgcggtca agacgacctg ctgcataaac gccctgaacc
    20221 gggatgaatt cttttaatgt tttgcgtccg gcttcatcgg tgaactgcgg aagaacatcg
    20281 tagggtttat tgaacaggat cacacgcgtg ggctggtttt caggtttacg cctggtagaa
    20341 cgttgtgagc tgaatcgctt aacctggtga tttctaaaag aagttttttg catggtattt
    20401 tcagagatta tgaattgccg cattatagcc taataacgcg catctttcat gacggcaaac
    20461 aatagggtag tattgacaag ccaattacaa atcattaaca aaaaattgct ctaaagcatc
    20521 cgtatcgcag gacgcaaacg catatgcaac gtggtggcag acgagcaaac cagtagcgct
    20581 cgaaggagag gtgaatggaa agtaaagtag ttgttccggc acaaggcaag aagatcaccc
    20641 tgcaaaacgg caaactcaac gttcctgaaa atccgattat cccttacatt gaaggtgatg
    20701 gaatcggtgt agatgtaacc ccagccatgc tgaaagtggt cgacgctgca gtcgagaaag
    20761 cctataaagg cgagcgtaaa atctcctgga tggaaattta caccggtgaa aaatccacac
    20821 aggtttatgg tcaggacgtc tggctgcctg ctgaaaccct tgatctgatt cgtgaatatc
    20881 gcgttgccat taaaggcccg ctgaccactc cggttggtgg cggtattcgc tctctgaacg
    20941 ttgccctgcg ccaggaactg gatctctaca tctgcctgcg tccggtacgt tactatcagg
    21001 gcactccaag cccggttaaa caccctgaac tgaccgatat ggttatcttc cgtgaaaact
    21061 cggaagacat ttatgcgggt atcgaatgga aagctgactc tgccgacgcc gagaaagtga
    21121 ttaaattcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa cattgcggta
    21181 tcggtattaa gccgtgttct gaagaaggca ccaaacgtct ggttcgtgca gcgatcgaat
    21241 acgcaattgc taacgatcgt gactctgtga ctctggtgca caaaggcaac atcatgaagt
    21301 tcaccgaagg cgcgtttaaa gactggggct acgagctggc gcgtgaagag tttggcggtg
    21361 aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaacaccggc aaagagatcg
    21421 tcattaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt ccggctgaat
    21481 atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgcc ctggcagcgc
    21541 aggttggcgg tatcggtatc gcccctggcg caaacatcgg tgacgaatgc gccctgtttg
    21601 aagccaccca cggtactgcg ccgaaatatg ccggtcagga caaagttaac ccaggttcta
    21661 ttattctctc tgctgagatg atgttacgcc atatgggttg gactgaagcg gctgacctga
    21721 ttgttaaagg tatggaaggc gcaatcaatg ccaagaccgt aacctatgac ttcgaacgtc
    21781 tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgacgcgatc atcaagaata
    21841 tgtaatcact acatgtgtta aatattgaaa cgggcgtata acacgcccgt tgttttatga
    21901 tgatttaaaa tcttccccaa aactttcccc aaaacccttc cccaaaactg gctattttct
    21961 atgctgtttt gatatctacg ataatccagt ctttaccacg atcatcattg tatcggtcgg
    22021 tcatttttct ggatttatga cctaacaact tttgcgtatc cagaccctgt tcccgatata
    22081 accgttctga cagagatcgc tgctcatgaa atgtgggcgc agttccttgc tcccatttta
    22141 tgccacattt ttccctggcc tttttaaaag ccgttgtaag agtatttgca gacacctggt
    22201 ctcctctgtt tgcttgagag gtagtgtgac ggtaatggac cagatattta ctaacaacag
    22261 catccctgca ctgagatata acttcacgaa gggtaatatt cagagcatcg catttcaggt
    22321 taagcggaat agcaagtttt gaaccggttt tttcctgagt aatgtgcaac atgtcgtccc
    22381 agatatcaga gaatttcaaa ttgcagatat cgcttaaacg ttgtccagtg acaagagcaa
    22441 gtagcatccc gcattttaaa tagggctgcc gtctgcttac gctgtcaaat attgcctgcc
    22501 attcgggcag tgataatctt tgacggttta ctcgatttcg cggttgtttt gttgcctgcg
    22561 ctgggttaaa tcctggcgga acatgtcctg cgtgttgtgc ttctttgaag acgtcgatca
    22621 acaccattct cacgacttgc gccatcctgt tatgaccttc agcctttaca gcatcaatta
    22681 tttcggcaat atcaagtgcg gtaatatcct tgaggtgttg cattccacaa tgctcacgga
    22741 aaagacggat gggtttgcct ttttgccgat aggagttggg tcttagttca ttatgttgca
    22801 gcctgtcctc ctggatagaa ttatatttat caagccattc tgtcaccgta atgtctgagc
    22861 gcctgccttt cattctttcc agacgctcat tgacgcttaa tatttgtcgg gtacgttgtt
    22921 cagcaataat ggtatttgct tcagtagcaa cttgttttgc ttcattctca tcagttccta
    22981 agctatgaaa acgaccggat agtggatgtt tgtattgcca atataccttt ccggttcgct
    23041 tatctaattt gcaatataaa ttgggtatag agattttgtg agatcggggg ctagcagcca
    23101 tcagcgatta tccgttggag ttttgggttt gcgtttattg ggagttgcgg ttctgcaagc
    23161 gttcctacaa aacgggaatt tcggtcaatc atccagtagc gaccaacttt tatagcgggt
    23221 ggggccatca ttttcccttg cgcgtatttt ttcagaactc gctcacttgg tgctaagtcc
    23281 ccaaattctt ctttagccca gtcccgtaaa gtgattagtc gagacatttg tcctcctctt
    23341 agctgctgag ggagtttgtg accgatatat ctgacatgat attaagctca tggcaggtac
    23401 atctcttgac tggtcataga gataaattta atgctgagaa atgcagtatt gaatttatca
    23461 atttttcacc agcttctggt ctttctggct gtcatgcgca aaacatatgg ctttaataaa
    23521 aaactggatt gggttagcaa cgagcaactt tccgaattaa ccgggatatt gccgcacaag
    23581 tgttctgctg caaaaagtgt tctggtaaag cgtgggattt ttattcagag cgggcggaat
    23641 accggcatta ataatgtggt cagtgaatgg tcaacattac ccgaatcagg taagaaaaat
    23701 aaagtttacc tgaaagaggt aaatttacct gaatcaggta aaaaaagttt acccaaatca
    23761 ggtaaaggcg tttacccgaa tcaggtaaac acaaaagaca aactaacaaa agacaatata
    23821 aaaccttttt cgtccgagaa ttctggcgaa tcctctgacc aaccagaaaa cgatcttcct
    23881 gtggtgaaac cggatgctgc aattcagagc ggcagcaagt gggggacagc agaagacctg
    23941 accgccgcag agtggatgtt tgacatggtg aagaccatcg cgccatcagc cagaaaaccg
    24001 aattttgcag ggtgggctaa cgata
//
LOCUS       DAZFKY010000063        23942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFKY010000063 DAZFKY010000000
VERSION     DAZFKY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..579
                     /locus_tag="ACHKCL_002176"
     CDS             46..579
                     /locus_tag="ACHKCL_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5763140.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            629..1312
                     /locus_tag="ACHKCL_002177"
     CDS             629..1312
                     /locus_tag="ACHKCL_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5763141.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1325..3775
                     /locus_tag="ACHKCL_002178"
     CDS             1325..3775
                     /locus_tag="ACHKCL_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5763142.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3788..4747
                     /locus_tag="ACHKCL_002179"
     CDS             3788..4747
                     /locus_tag="ACHKCL_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO5763143.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIAL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGLFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(4785..5684)
                     /gene="nhaR"
                     /locus_tag="ACHKCL_002180"
     CDS             complement(4785..5684)
                     /gene="nhaR"
                     /locus_tag="ACHKCL_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5763144.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5750..6916)
                     /gene="nhaA"
                     /locus_tag="ACHKCL_002181"
     CDS             complement(5750..6916)
                     /gene="nhaA"
                     /locus_tag="ACHKCL_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5763145.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            7502..7654
                     /gene="mokC"
                     /locus_tag="ACHKCL_002182"
     CDS             7502..7654
                     /gene="mokC"
                     /locus_tag="ACHKCL_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5763146.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(7758..8888)
                     /gene="dnaJ"
                     /locus_tag="ACHKCL_002183"
     CDS             complement(7758..8888)
                     /gene="dnaJ"
                     /locus_tag="ACHKCL_002183"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5763147.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8977..10893)
                     /gene="dnaK"
                     /locus_tag="ACHKCL_002184"
     CDS             complement(8977..10893)
                     /gene="dnaK"
                     /locus_tag="ACHKCL_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5763148.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            11270..11674
                     /gene="yaaI"
                     /locus_tag="ACHKCL_002185"
     CDS             11270..11674
                     /gene="yaaI"
                     /locus_tag="ACHKCL_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5763149.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11700..12413
                     /gene="msyB"
                     /locus_tag="ACHKCL_002186"
     CDS             11700..12413
                     /gene="msyB"
                     /locus_tag="ACHKCL_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5763150.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            12562..13128
                     /gene="satP"
                     /locus_tag="ACHKCL_002187"
     CDS             12562..13128
                     /gene="satP"
                     /locus_tag="ACHKCL_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5763151.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(13163..13750)
                     /gene="mog"
                     /locus_tag="ACHKCL_002188"
     CDS             complement(13163..13750)
                     /gene="mog"
                     /locus_tag="ACHKCL_002188"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5763152.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(13865..14818)
                     /gene="tal"
                     /locus_tag="ACHKCL_002189"
     CDS             complement(13865..14818)
                     /gene="tal"
                     /locus_tag="ACHKCL_002189"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5763153.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            15097..16527
                     /gene="yaaJ"
                     /locus_tag="ACHKCL_002190"
     CDS             15097..16527
                     /gene="yaaJ"
                     /locus_tag="ACHKCL_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5763154.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            16597..17373
                     /gene="yaaA"
                     /locus_tag="ACHKCL_002191"
     CDS             16597..17373
                     /gene="yaaA"
                     /locus_tag="ACHKCL_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5763155.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(17526..17822)
                     /gene="yaaX"
                     /locus_tag="ACHKCL_002192"
     CDS             complement(17526..17822)
                     /gene="yaaX"
                     /locus_tag="ACHKCL_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5763156.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(18036..19322)
                     /gene="thrC"
                     /locus_tag="ACHKCL_002193"
     CDS             complement(18036..19322)
                     /gene="thrC"
                     /locus_tag="ACHKCL_002193"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5763157.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(19323..20255)
                     /gene="thrB"
                     /locus_tag="ACHKCL_002194"
     CDS             complement(19323..20255)
                     /gene="thrB"
                     /locus_tag="ACHKCL_002194"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5763158.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(20257..22719)
                     /gene="thrA"
                     /locus_tag="ACHKCL_002195"
     CDS             complement(20257..22719)
                     /gene="thrA"
                     /locus_tag="ACHKCL_002195"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5763159.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(22745..22858)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(22800..22883)
                     /locus_tag="ACHKCL_002196"
     CDS             complement(22800..22883)
                     /locus_tag="ACHKCL_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5763160.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(23097..23783)
                     /gene="yjtD"
                     /locus_tag="ACHKCL_002197"
     CDS             complement(23097..23783)
                     /gene="yjtD"
                     /locus_tag="ACHKCL_002197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5763161.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         6148 a         6005 c         5625 g         6164 t
ORIGIN      
        1 tttgaaaacg agtgaccttc cataccagtt aattaaggat gatttatgaa aaagtatatt
       61 attcccttcg ttgcgacaac gttgatgttt agcgcaacaa ccttcgccaa ggatgtcgat
      121 caaggaacac taaccattag aggagaagtt gttgatacaa cgtgttactt tgttaatggt
      181 gattcgacaa cagatatcgt tgtcgaagat gttgtaacca gtgcaatgaa tcaacttatc
      241 aataatgaaa tctatacttt aaaaaccggt tcaacctctc atgacttaga aattcagtgt
      301 gacggaaata cagccccaag tatcgaagta ctagcatcag agtttgatgc taataacttt
      361 acgctgaata aaggaactgc tgagaacatt ggctttgcca ttttcctcga tgatgggact
      421 ttgaccagaa tgagaccagg gttaaaaaca ccgctgaaag ataatggctc tggtcaatat
      481 tttctcaatt taaatgcaca atatgcccgc acaagtggca atcctgttac aaaaggctcc
      541 gtagagtcaa ccgtaacgat taaaatcagt gcagattaac gttttttgcc ggtagctctg
      601 ctaccggtat tatttacgga gtgattacgt gagaataata acagcattac tgatgtcatt
      661 ttttatttta cctgttcatg ccggtgtggt catctatggt acgcgcatta tttatccgga
      721 aaaaaacaga gaagttctcg tacagttaat gaatcaaagt aaaaatgcct ctttgattca
      781 ggcatggatc gatgatggga acaccactat cgcgccagaa aaaattcagg tcacatttat
      841 tctcacaccg ccagtaagtc gtgtagcagg tggttctggc caacaactta aaataagaaa
      901 aatgccaaac aatttgcctc acaataaaga aagtttattt tatctaaatg ttctggatat
      961 ccctccaaac aatccacaaa atgcagggaa aaacaaaatt aaacttgcat tgcaaaaccg
     1021 gataaagtta ctttggcgtc caagcggaat agctccggta gataaaaaaa gcctttctca
     1081 attaaatatc aaaaagaaaa ataacgccat ctcaataaac aacgaaacag ccaactggat
     1141 aacggttaca actatcaaag cacaaaacgt gaaagttaat aatgaaagta tcttactgcc
     1201 tcccttttcg cataacgaca ttacattaaa aaataaccac gcatcagaat atgaactaac
     1261 ggtagttgat gattacggga ataacatcca tagtaaaatc gccgcgagat aacaggaaaa
     1321 agtcatgtta aggacgacac gatgggtcgc tgctattatt tttttgtatt cttttccggg
     1381 ttatgctgaa gaaacttttg atacgcactt tatgatcggc ggcatgaaag gagaaaaagt
     1441 ttccgaatat catttcgata ataaacaacc gcttccagga aactacgaac ttgattttta
     1501 tgtaaataac cagtggcgtg gaaaacaaga tatcacgatc ccagagtcac cagtcaaacc
     1561 atgtctgcct aaggtgctac taaccaaact gggggtaaag accggtaacc tcaatacaga
     1621 agataattgt attttactgg ataaagcggt tcatggtgga caatatcagt gggatatcag
     1681 tgaacaccga ctaaatttaa cagtaccaca ggcgtatatc aatgaacttg aaagaggtta
     1741 cgttcccccg gaaagttggg atcgcggcat tgatgctttt tatacctcct ataatttaag
     1801 tcagtatcgc tcttatgaca gcaataataa cagtaataca gccagttatg gccgatttaa
     1861 cagtggatta aatttattta gttggcagtt acactctgat gccagctata gcaaacccga
     1921 tgatatgaaa ggcacatggc aaagtaatac tctttacctg gaacatggct ggtcgcagat
     1981 attaagcaca gttcagatag gagaaaatta cacatcctca ctcatattcg actctctgag
     2041 atttagcgga atacgcttat ttcgcgatat gcaaatgcta cctgattcta tgcagagctt
     2101 tacaccttta gtacaaggtg ttgcgcaaag taatgccttg ataacggttt cgcaaaatgg
     2161 ctacatcatt taccaaaaag aggttccccc gggaccattt actatcgcag atttgcaact
     2221 ctcgggcagc ggctctgatc tcgatgtcag cataaaggaa gcagatggca gtgttcgttc
     2281 ctttttggtg ccctactctt ccgtgcctaa catgctgcaa cctggtattt caaatttcga
     2341 ttttatcgcc ggacgcagta agatatatgg tgtaaagaat caggaggact ttctcgaggc
     2401 aaattatata tatggcctca acaatctttt gacactttat ggcggtacga ttttatccga
     2461 taactataat gctataactc tgggaaatgg ttggaataca ccgctgggtg ccatctcttt
     2521 tgacgctaca cgatcgagta gtaagttaaa taatgatata acacacgaag gtacgagcta
     2581 tcaggtcgcc tataataaat atctagtaca aacggccact cgatttagcg tggccgcctg
     2641 gcgttatgct tcgcaggatt acaggacatt cagcgaccat ctttacgaaa atgataaaat
     2701 aaatcatcag agtgactatg acgattttta tgatattggc agaaaaaata gcctttctgc
     2761 caatatcatg caacctttat ccaataatct gggaaatgta tcattaagtg cgctttggcg
     2821 gaattactgg gggcgaagtg gaaatgctaa agattaccaa ttcagttatt ccaatagctg
     2881 gcaacgcatc agttatactt tctctgccag ccaatcctat gatgaaaacg acaaagaaga
     2941 ggagcgtttt aatctgttta tctccattcc tttctactgg ggggatgata ttgccaaaac
     3001 acgtcaccaa attaacttat cgaattcgac ctcattttcc aaagatggct attcctccaa
     3061 caatactgga attactggca tagccggtga acatgatcag ttaaattatg gtatatatgt
     3121 taatcagcaa caacaaaata atgatacatc gcttggtacg aatttaagct ggagaactcc
     3181 catcgccaca atagatggca gctatagcca ttctaaaaac gcctggcaaa gtggtggaag
     3241 tattagtagt ggattagttg tctggcccgg tggtattaat atcactaacc agctatccga
     3301 tacatttgca attctggatg cgcctgggtt agaaggcgcg catattaatg gacaaaaata
     3361 caaccgaaca aacagcaaag gccaggttgt ttacgacctg atgatacctc atcgtgaaaa
     3421 ccatctggta cttgatacag caaacagtga aagtgaaaca gaattgcagg gcaatcgtca
     3481 aattattgcg ccttaccgtg gagcagtttc ttatgtgcag tttacaactg accaacgtaa
     3541 gccctggtat atacaggcac tgcgtcccga tggttcgcca ttaacttttg gctatgacgt
     3601 actggatctc caggaaaaca atattggagt cgttggccag ggtagtcgcc tttttattcg
     3661 cgtagatgaa attccaactg gcataaaagt tgctctcaat gatgaacaga atttattctg
     3721 tactattact tttcaacacg ttatcgatga aaacaaaaca tatatatgcc agtaaaagga
     3781 gtttactatg aagtggcttt tattaatcac attgagtcta tattctttta ttgtgcaaag
     3841 tgctccctgt ggattgacca atgtcgggga acaacgtgga acatatatac tcaaaccact
     3901 gagtatgaaa ggtaatctca ccgccgaaaa aattgaaaat actgggaata tcattgagtg
     3961 tgcagacttg caagagacga ccaacgccat taaagttcat tttttaacac agaacgcttt
     4021 aaggtttaaa gtcggaagtc gtcattatat tattaatttc ccctttgaat ctgacggtaa
     4081 aaaaatagcg cttccaggga agtcatctta taatttagaa gaaatgctat ctgcgataaa
     4141 tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg cagatatttt
     4201 tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta cagggaataa
     4261 tactaattgt aatagcgtcc gctttaattt taatttaaat actattttac aggtaaattt
     4321 aatgacctgt ggttttgaag atcaatccat cgacactgga ctatttgtac tgtctcgtat
     4381 caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg gcgagcaagg
     4441 tgaattagtt tttgaaccca gtgacgttga atattacttt gaaccagtga cgatacagga
     4501 gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg cgggtcaaat
     4561 aggctttcaa ctctccaatg atggtacgaa tgaaatccag tatggtaaaa gcaattacta
     4621 ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc gcccgcgtac
     4681 atatggaaac aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg tggtaatgta
     4741 caactaatct atctccccca ccttaacttt gggggagctg ccgattaacg caccgctgga
     4801 ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg ctgaatcatc
     4861 cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc aatttctacg
     4921 acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa gattgcattg
     4981 tgcatcgcac caaaagcttt catcaaagcg gcatcatcaa actcgccaag gatttctacg
     5041 tttaatccct gggagttaaa ccagttaagt aatttgcgcc ctaacattga acgtcgccca
     5101 ggaatcaaaa gccgccgttc ttcaagacaa gctgggaacg gtttttccgg cggtggattt
     5161 gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc ttcctgctgc
     5221 gtagaatcta tcggacagtc ggaaatgatc atatccagtt tatgctggct taattgctcc
     5281 agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tcggctcgcc ttctactact
     5341 gcagcattca gcacgctact gaccagacgt ttggaaagta catcagccac gccaacgtca
     5401 aacaataaat tggattcttt gcgatagttc acgatatcca gcatttcctg gctcaaggta
     5461 aacattttat cggcatagcg atagaccagc tcccccagct cgctgggctc gagaccacgt
     5521 cccttgcgct taaataattt gccttgcagg cgctcttcca gcgcccgaat ctgtccggta
     5581 atggtttgtg gtgttaaata aagcgcctcc gctgcgccaa ccacagaacc ttctttataa
     5641 acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat ttctctccct
     5701 gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt caaactgatg
     5761 gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata gaaccgacca
     5821 ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag gccaggctgg
     5881 caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc ataatttgct
     5941 gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac cagcagaaca
     6001 gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat ggcagaatgg
     6061 aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca aatgcaaaca
     6121 gtggtaaaat cagatacgcc acccacgggt gcaacacatg ctccagtcgc ttcgctggag
     6181 aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc gccagggttg
     6241 cgtgaacccc cgatttcaac actgcagtcc acaacactac gccaaccaga atatagacgc
     6301 ccgtgcggcg tacaccacac agattcaata ccgcgagtac agcaattgct acagccgcga
     6361 cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata atgatggccc
     6421 caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac ggaacacgac
     6481 ttcccaatag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc gggatcgccc
     6541 acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat gccggcacaa
     6601 tcatcccacc aatagcagcg ataactggaa atgcggcctg gcgtaagctg gctagcgatc
     6661 cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc gccatcagcg
     6721 cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc cggagctgaa
     6781 ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg gccatcatca
     6841 tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg ctaaagaatc
     6901 gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt tactatttta
     6961 ccccgcacga atcatcgtta aaatcagatt atagtgtagg aataattcgt tttttacgat
     7021 ctatagaaga atttgcgcca tcagcggtga gatgcgtttt tgacggctgt aagcgctgac
     7081 gggtgaccct aagccctgat aatcatagct taaggtgagg atttctgagg gattaattag
     7141 gaaaagtaat aattaatttg ttgtcagtcg gcgatgtttg ttaggccaat atttcaaaaa
     7201 taatccagct tgtcgtcaat aattgcgatg aagagataaa ttaaagttaa ataattaaat
     7261 cattaaaaaa ttacgccata aaaatgcgag gcgtcttccg gattagaatc gataaaacag
     7321 aatgggttaa agagagcgat ataatagcgg cgggtgcttg aggctgtctg tctcaggcat
     7381 tagctgaacg gcagatagag aaaagccccg agtgatattt taccatcaac ccgaggcccc
     7441 ctatatgctg aacacatgta gagtgcctct tactgaccgt aaggtcaagg agaagagagc
     7501 aatgaagcag cataaggtga tgattgtcgc cctgatcgtc atctgtatca ccgccgtagt
     7561 ggcggcgctg gtaacgagaa aagacctctg tgaggttcac atccgaactg gccagacgga
     7621 ggttgctgtt ttcacggctt acgaatccga gtaagagcaa cggcggggag tgatccccgc
     7681 cactttacag gtgctcgcat atcttcaacg caccctattt ttacccaggc ctgcccacgg
     7741 gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac accatcaaag
     7801 aagctctttg agcgcgggct gttgtgctcg ccggttgggc caccgaagct ttcttgcagc
     7861 tcttgcagca gctgtttctg cttctcgttc aggcctaccg gtgtttcgac gacaacgcgg
     7921 cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc gcgcatacgg
     7981 aatagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc atcaagggtc
     8041 ggtacttcga tttcaccacc cagcgccgcc atagcgaagt tgatcgggac ttcgcaatac
     8101 aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg aacgtacaga
     8161 tcgcctgccg gtgcgccgtg ttcacccgct tcaccttcgc ccgcaagacg gatgcggtct
     8221 ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc aacacgacca
     8281 tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg acagtgtgga
     8341 caggtctgct gcacggcaaa gaaaccctgg cgcatctgca cctgaccaga accatgacag
     8401 gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca aacgtcacac
     8461 tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc ttcgagggtg
     8521 agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc acgtccgccg
     8581 ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc gccgccaaaa
     8641 ccgccgccgc ccatgccacc ttgctcaaac gcagcatgac catactgatc gtatgccgca
     8701 cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa tttcgcctcg
     8761 gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg tttgtaggcc
     8821 tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta ataatcttgc
     8881 ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga aattcccctt
     8941 cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt tgacttcttc
     9001 aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat cagcaccggc
     9061 agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg aaacctgtgc
     9121 cagttcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag cagtttccag
     9181 tgcagtcagc gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt cgcctgcttc
     9241 ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag tctgtaccag
     9301 ctcttcaaac ttacggtcag cttcggcgtt agcttcggcg tcgcgtacca ttttctggat
     9361 ttcatcttcg ttcagaccag aagacgcctt gatggtgatc ttctgctctt taccgctgtt
     9421 tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg taacttcgat
     9481 ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac ccagagattt
     9541 gttgtcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg cagactggtt
     9601 gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt ttttcgcgat
     9661 cagcgtcgtc atcacaccgc ccatggtttc gatacccaga gacagcgggg taacatccag
     9721 cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag caccgattgc
     9781 tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact cagcaacttt
     9841 cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt cgatatcaga
     9901 tacggacagg ccagcgtcct gcagcgcaac tttcagcggc tcgatggaac ggttaaccag
     9961 atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt gtttcggacc
    10021 ggtcgcgtct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg aagacagttc
    10081 gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat cgttgcgcag
    10141 gtcaatgccc tgatctttct tgaattcttc aaccagatag ttgatcagac ggctgtcgaa
    10201 gtcttcacca cccaggtggg tatcaccgtt ggttgccaga acttcgaagg ttttttcgcc
    10261 gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccaccaccca agtcataaac
    10321 cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag ctgcggtcgg
    10381 ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt tggttgcctg
    10441 acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta ccggttcacc
    10501 caggtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaaa tctgcggcgg
    10561 tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag caataatttt
    10621 gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc ggccaatcag
    10681 gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt tagccggctg
    10741 accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg tgcgatcgcc
    10801 ttcggcgttc tccagcacac gaggagtggt gccatccata atcgctacac aagagttggt
    10861 agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat atattcggtc
    10921 atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg cccaatttca
    10981 tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac ccacgtcatc
    11041 aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat atggcaggac
    11101 aagacgtaaa attgacagca attttattca tataaagaat gaatcgcagt caaggtattc
    11161 atcaaaaaat tgtaataaaa agaaaagatt acgtgcctga atcttctctt tatcagcagt
    11221 aaactagtgg gtattcatcc ccctacctct tcccactaag agaatcctta tgaaatccgt
    11281 ttttacgatt tccgccagcc tggcgattag cctgatgctg tgctgcacgg cgcaggcaaa
    11341 cgaccataaa ctcctcggcg tcattgcaat gccgcgtaac gaaaccaacg ctctggcgct
    11401 gaaacttcct gtttgtcgca ttgtgaaacg catacaactc tctgccgacc atggcgattt
    11461 acagttaagc ggtgcatcgg tttatttcaa agccgcccgt agcgccagtc agagcctgaa
    11521 tattccttca gaaataaaag aagggcaaac cactgactgg atcaacatta acagcgataa
    11581 cgacaataaa cgctgcgtct caaaaatcac cttttccggt catacggtga actcatcgga
    11641 tatggccacg ctgaaaatta tcggcgacga ctaacgccag aatatagaag ccacaaaaaa
    11701 tgaatgttaa ttacctgaat gattcagatc tggattttct ccagcattgt agtgaggaac
    11761 agttagcaaa tttcgcccgg ctgctcaccc ataatgaaaa aggcaaaact cgcctctcca
    11821 gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg acatcccgag caacatcgcc
    11881 gcaactggca gctgattgct ggagaattac agcattttgg tggcgatagt atcgccaaca
    11941 aactgcgcgg acacggtaaa ttgtatcggg ccattttgct cgatgtttca aaacgattga
    12001 agctgaaagc cgacaaagag atgtctacgt ttgaaattga gcaacagtta ctggaacaat
    12061 ttctgcgtaa tacctggaag aaaatggacg aggaacataa gcaggagttt ctgcacgcgg
    12121 tcgatgccag ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa gacaaattat
    12181 tggcaaaagg cgtgtcgcat ctgctttcca gccaactgac ccgcatttta cgcacccacg
    12241 cagcaatgag cgtacttggg catggtttgc tgcgcggcgc ggggctggga ggccctgtag
    12301 gcgcggcact aaatggggtt aaagcggtca gcggcagctc ctatcgcgtg acgattccag
    12361 ccgtactgca aatcgcctgc ctgcgccgga tggttagcgc cactcaggtc tgataccgga
    12421 aaccggggaa aattttcatt ttcccctcaa aagatcgtag acactgcccc actggctgat
    12481 tattatgccg cgccctgaaa acactacagt tattcaggga aattatttca ccattcattc
    12541 gatgatgatt tttgaggaat tatgggcaac actaagttgg ctaatccggc accgctgggc
    12601 ctgatgggct tcggcatgac caccattctg cttaacctgc acaacgtagg ttatttcgct
    12661 ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca aatttttgct
    12721 ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac ctcttacggt
    12781 tctttctggc tgacgctggt tgcgattctg ctgatgccga aactgggtct gaccgatgcg
    12841 ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcgtatttac gctgtttatg
    12901 ttcttcggca cgctgaaagg cgcacgcgtt ctgcaattcg ttttctttag cctgaccgtg
    12961 ctgtttgccc tgctggcgat cggtaacatt gccggtaacg ccgcaatcat ccactttgcc
    13021 ggctggattg gtctgatctg cggtgccagc gcaatctatc tggcgatggg tgaagtactg
    13081 aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt gtcttgagat
    13141 ccccccgctc ggggggattt ttttattcgc taacgtcgcg tcttgcactc ttcggtctga
    13201 atgcagcaac cacttctggt gccgtttcaa cgtatggccc ttccagcaac tgaatgcagt
    13261 acggtacgct ggcaaaaata ccgtgtacca caacgttacc ctcagcgtcc ttcacacctt
    13321 ccagcgtctc tttaatagac ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc
    13381 gaatcacacc cacctgacgc gaaaggatcg cagttggtac aaaatgcagg ctgatctggc
    13441 gcatctgttc accaaagcca ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg
    13501 ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag caccagatgg caactcattt
    13561 catccaccag ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg attaagcggg
    13621 tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagc gcagggatac
    13681 ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag ccaatacgta
    13741 aagtattcat caagattttt ccgtttaaac agtctcgtta aacagaatga tacactgcga
    13801 agggagtgac agacaggctt cagaagaggt agcgtgaccg acttcccggt cacgctaaga
    13861 atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaata gcaaacttac
    13921 ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc cacaggaact
    13981 cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt
    14041 cgatagcccc ttcgctctca gccagctctt tcagcagtgc cggtgcgatg gtcagacggt
    14101 cgcagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc ataaccacgg
    14161 tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc acgcccggat
    14221 cttctgccgg agcgtactct ttcttatcgg tattcgcttt gtaccagtca agaatacggc
    14281 caacaaacgg cgagatcagg aacacgcccg cttccgcaca agcacgagcc tgagcgaagg
    14341 agaacagcag ggtcaggtta cagttgatac cttctttttc cagctgttcc gcagcacgga
    14401 taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata cctgcatcgt
    14461 tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttctgtgtca taggaaagac
    14521 gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc agaccaatat
    14581 ttactgccag tttgtcagtc gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt
    14641 tcgcccaggc gacagcatca tcaatcaact tacggtattc cggaatctgc gctgcgttaa
    14701 gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt gccgcgatgt
    14761 ccccagtgtc ggccactacg gtggtgtact gacgaaggga ggtcaatttg tccgtcatga
    14821 tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct gatgatatca
    14881 cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga ctgtttcgga
    14941 tttctgacac tgcgttgata tcgcccgcca tttttaaaca aaacctcatg tatgctacgc
    15001 agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa cgtttacaca
    15061 ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt ctcctttatt
    15121 aacagcgtcc tttggggatc ggtaatgatt tacctgctct tcggcgcagg ttgttggttc
    15181 acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa
    15241 aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg taccagtctt
    15301 gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggctattac cgccggtgga
    15361 cctggagccg tcttttggat gtgggttgcc gcgtttatcg gcatggcgac ctcgtttgcc
    15421 gaatgttccc ttgcacaact ttataaagaa cgtgacgtca atgggcagtt tcgtggcgga
    15481 ccggcatggt atatggcgcg cgggctgggg atgcgctgga tgggcgttct gttcgccgtc
    15541 tttttgctca tcgcctacgg cataattttc agcggagttc aggcaaacgc tgttgcccga
    15601 gctctgagtt tttcttttga ttttcccccg ctggtgacag gcattattct tgctgtcttt
    15661 gctctgctgg caatcattcg cggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc
    15721 ccgttgatgg cgataatctg ggtactgacc agcctggtga tttgcgtaat aaatatcggg
    15781 caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca ggaagcggca
    15841 ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca gcgcagtatg
    15901 ttttccaatg aggcgggaat ggggtcgacg ccaaacgcgg cagcggcagc ggcgtcctgg
    15961 cctccgcatc cggcagcgca aggaattgtc cagatgattg gcatttttat cgacaccctg
    16021 gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacggcac aacctacatg
    16081 ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg ttcctggggt
    16141 gctgaatttg ttaccctcgt ggttattctg tttgccttca gctccatcgt tgccaactac
    16201 atttatgccg aaaacaatct cttcttttta cgcctgaaca accctaaagc gatctggtgt
    16261 ttgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagtct tccgctgatg
    16321 tggcaactgg cagatatcat aatggcctgc atggctatta ccaatttgac tgccatatta
    16381 ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg taaactcggc
    16441 gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg gtcgccagct ttctcgggac
    16501 gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt ctcttttttt
    16561 gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat ttcacctgcg
    16621 aaaacgcttg attaccaaag cccgttgacc accacgcgat atacactgcc ggagctgtta
    16681 gacaattccc agcagttgat ccatgaagcg cggaaactga cgcctccgca gattagcacg
    16741 ctgatgcgta tcagcgacaa actggcaggt atcaacgccg ctcgctttca tgactggcag
    16801 ccagatttca cgccggagaa tgcccgccag gcgattctgg cgtttaaagg tgatgtctac
    16861 accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca acagcatttg
    16921 cgaatgcttt ccggcttgta tggcgtactc cgcccgctcg atttaatgca gccttatcgt
    16981 ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca attctgggga
    17041 gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt
    17101 aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa tgccgagatt
    17161 atcaagccgg tgttcctcga tgagaagaac ggcaaattta agataatcag cttctacgct
    17221 aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac caaaccagag
    17281 caactgactg gttttaatag cgaaggttac ttctttgatg aagcttcctc cagcaatggc
    17341 gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg gatgcggcgt
    17401 gaacgcctta tccggcctac aaaagcatgc agattcaata tattgcagaa ttcacgtagg
    17461 cctgataaga cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta cccggcattt
    17521 gtcatttagc ggtgatgttt gcctggacca tgaccgccgt gatgatcatg atgagctttc
    17581 ttatggtgac gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca
    17641 taatgttgtt tccaccagcc gtggtcgcgc cagtgaccgc catcccagta ataaccgcga
    17701 ttatcacgat cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgc
    17761 gttgccatgg gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc
    17821 acaggttact ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag
    17881 agcggcggtt gttattctcc gcaatcctaa tctctaagtt accgcattta ttgagaattt
    17941 ctcctttttc cctgtcgttt ctctccatat tttcttcaca aaaaagccgg gctgcataaa
    18001 agcaaacccg gcctgattga gataatgaat agatgttact gatgattcat catcaattta
    18061 cgcaacgcag caaaatcggc gggcagatta tgcgaaagca agggtaaatc ggcacgttct
    18121 gccagctctt ttggcagatc caacgtttca ccgagaatcg cttccacgct ctctttaaat
    18181 ttcgccggat gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc
    18241 agcgcacgat acgctacggc agcgtgcggc tccgaagtgt agcccagttc ttttaactca
    18301 cgcattgtct gttgcgtggt ttcatcatcc acggcggcat aacccagctc tttcagttgc
    18361 cagattttgc ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc
    18421 atcgcgttgg ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc atgcaggaaa
    18481 cgtggcacgg tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgagt
    18541 gacttcgcca gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc
    18601 agctggttgc gcgcttcctg cggcagctgc gcaactgctt caaagtagta gcaaatctgc
    18661 gccagcaacc ggctaatgtt aatcgagtta gctgagttta accccagcgc cactttcagc
    18721 tcttcatcat caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg
    18781 gcaacagttt cgatattgcc gcccaatgta cagaacagtt tttcttgcag tggactgatt
    18841 ttgcctcgtg gataaaggat aaccactttc acattcggta aaccgtagaa agcatgagcc
    18901 actgccgctc cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcgcccgca
    18961 atatgggtca gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc
    19021 ccgtggaaca attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag
    19081 gcaaacgccg cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc accaataaac
    19141 gccgagagga tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca
    19201 gtcaggctga attccggcag gtcgtgcgga aaaaacagcc cctgattttt gcccaacccc
    19261 tgggttacgg cttgcgcaaa gctgacctgc tcattgtgat ctttaagatt gtagagtttc
    19321 atttagtttt ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct
    19381 tcctgatttt gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc
    19441 ttgtcacaca gagcaaacaa ggtcgggccg gagccggaga taccgctcgc taccgcgccg
    19501 atttccgcga ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc acggtagggt
    19561 tctgcgataa catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg
    19621 tgaatgaagc ctgccaagtg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc
    19681 ggtaaaatag cccgggcttc tgccgtcgag actttaatcc ccggatacgc cagcacccac
    19741 agccactcat caaaccctgg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac
    19801 tgcataccac caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagattcgt
    19861 ccttccagct cgcccatcaa agccagcaaa cgggtgtcat taagcggctt gccgcagtgt
    19921 tcattcatcg ccatcagagc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg
    19981 atcggcatat tcttttccag agtcatcgcc actggaattt gcttgcccag ctcctggcaa
    20041 aaacgctccc agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca
    20101 aagcgtccga ggttgttgag actgaatgtc tctgccgact caaccgtgac tacatctccg
    20161 agcaatgcac catcaacggg tgtcaccgcc gccccgagca catcaaaccc gacgctcata
    20221 ttggcactgg aagccggggc ataaacttta accatgtcag actcctaact tccatgagag
    20281 ggtacgtagc agatcggcaa agacaccggc agcggtaacg tcattgcccg caccatatcc
    20341 gcgcagcacc aacggcagcg gctgataata gtggctataa aaggccaggg cgttttcgcc
    20401 atttttcact ttgaacagcg gatcattacc atccacttcg gcaatcttca cgcggcagac
    20461 gccatcttca tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc
    20521 cacgcgcgcg gcaaagagat cgtcgagctg tgacagattc gccataaaag cggcaacatc
    20581 accctcagcg ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag
    20641 ttcacgtccc gtttcacgag cgagaattaa tagtttacgc gctacatcca taccagaaag
    20701 atcatctcgc ggatccggtt cggtataacc catttcccgc gccagcgtag tcgcctcgga
    20761 gaaactcatg ccttcgtcta acttgccgaa gatataagaa agcgaacctg aaagaatgcc
    20821 ggagaacttc atcaattcat caccagcatt gagcagattt tgcaggttct caataaccgg
    20881 taatccagcc ccaacgttgg tgtcatagag gaatttacgc cgcgattttt cagccgcatg
    20941 acgcaacaga tggtagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac
    21001 gtggaaacct tcgcgcagga agtcggcata ttgatccgcc actgcctggc tggaggtgca
    21061 gtcaacaatg accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag
    21121 attaaacggc tctttggctt gcgccagttc ttcctgccag ttttccagat ttaggccatg
    21181 cacattggtg agcagagcct tcgagttagc aacaccgcag acacgtaagt cgatatgttt
    21241 attcttcaac cagctttgct gacgcttcag ttgctccagc agcgcaccgc caacgccacc
    21301 gacgccaatc acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac
    21361 gcgcacgcca gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga
    21421 tccctgagca atagcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc
    21481 cgagatccca cgcaaggtgc gcataccatc acctaccacc gagataatgg ccagccgttc
    21541 cgtcaccgcc agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat
    21601 tgcccgttca gctcgcacac agtcgctttg cggaacgcag aaactgatac tgtattcgga
    21661 agatgattgc gtaatcagca ccacggaaat acgggcgcgt gacattgcag caaagacgcg
    21721 cgccgccatg ccgaccattc ctttcatccc cgggccggaa acgctgaaca ttgccatatt
    21781 attcagattg gaaatgccct tgaccggtaa ttcgtcttca tcacgactgg caccaatgag
    21841 cgtacctgga gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc
    21901 gatgggggta atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc
    21961 ttcctggtag gacatcgatt tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt
    22021 atataccccg tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag
    22081 caccgccgcg gagtagtcgg aaccgttgcg tccaagtacc accagttcgc ctttttcatt
    22141 accggcggtg aaacctgcca tcagcaccat gtgatcagcc ggaatacgac ttgccgcaat
    22201 acggcgggtg gactctgcaa tatcgacagt agattcgagg taatgcccca ctgccagcag
    22261 tttttcgacc ggatcgataa cggtaacgtt gtgaccgcgc gcttctaata cgccggccat
    22321 aatggcgatc gacattttct cgccacggca aatcagcgca gcgttaatgc tatccgggca
    22381 ctgccctaac aaactaatgc catgcaggac atgttttatt tgggcaaatt cctggtcgac
    22441 gaaagttttc aattgcgcca gcgggaatcc cggctgggcg gcggcgagtc ccgtcagaag
    22501 ttcggcaaaa atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt
    22561 tttttcaatc atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc
    22621 cacctgcccc tgcctggcat tgctttccag aatatcggca acccgcagaa aacgttctgc
    22681 atttgccact gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt
    22741 ggtcgaaaaa aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt
    22801 cagcccgcac cgttacctgt ggtaatggta atggtgatgg tggtggtgat ggtggtggta
    22861 atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat ctgtctgtgc
    22921 gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt taatttactc
    22981 acggcaggta accagttcag aagctgctgt cagagactct ttttttaatc cacacagaga
    23041 catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg cgttttttat
    23101 ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt gtctcgttgc
    23161 tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc tgccactgcc
    23221 agagtcgtca gcaatgccat ggctcgttcg cgtaaagctt gcagttgatg ttggtctgcc
    23281 gttgtgtcac ttttcgtcgg ttgttgtatt aatattgcta attgatagca atagaccatc
    23341 accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc agtaagaacg
    23401 tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg
    23461 gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac tggcgtagcg
    23521 tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc gacatcgtgt
    23581 aacgattcag ccagtgtcgg gaaaacttta atattatcaa taatatcacc agatccatgt
    23641 gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg cagttcgcta
    23701 aaccccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct ggcgggtgcg
    23761 accagaataa tcgttatacg catattgcca ctcttcttga ccaaatgacc gcgaacaggg
    23821 tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta agtcgcagaa
    23881 aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact aacggtttaa
    23941 tt
//
LOCUS       DAZFKY010000064        23751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFKY010000064 DAZFKY010000000
VERSION     DAZFKY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..3855)
                     /gene="entF"
                     /locus_tag="ACHKCL_002198"
     CDS             complement(<1..3855)
                     /gene="entF"
                     /locus_tag="ACHKCL_002198"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5763162.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGP"
     gene            complement(3852..4070)
                     /locus_tag="ACHKCL_002199"
     CDS             complement(3852..4070)
                     /locus_tag="ACHKCL_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO5763163.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4073..5275)
                     /gene="fes"
                     /locus_tag="ACHKCL_002200"
     CDS             complement(4073..5275)
                     /gene="fes"
                     /locus_tag="ACHKCL_002200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5763164.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5518..7758
                     /gene="fepA"
                     /locus_tag="ACHKCL_002201"
     CDS             5518..7758
                     /gene="fepA"
                     /locus_tag="ACHKCL_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5763165.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7933..8553
                     /gene="entD"
                     /locus_tag="ACHKCL_002202"
     CDS             7933..8553
                     /gene="entD"
                     /locus_tag="ACHKCL_002202"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5763166.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8675..8827)
                     /gene="hokE"
                     /locus_tag="ACHKCL_002203"
     CDS             complement(8675..8827)
                     /gene="hokE"
                     /locus_tag="ACHKCL_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5763167.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9178..9330)
                     /gene="hokE"
                     /locus_tag="ACHKCL_002204"
     CDS             complement(9178..9330)
                     /gene="hokE"
                     /locus_tag="ACHKCL_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5763168.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9299..9566
                     /locus_tag="ACHKCL_002205"
                     /pseudo
     CDS             9299..9566
                     /locus_tag="ACHKCL_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9769..10887
                     /locus_tag="ACHKCL_002206"
     CDS             9769..10887
                     /locus_tag="ACHKCL_002206"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5763169.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10953..11201
                     /locus_tag="ACHKCL_002207"
     CDS             10953..11201
                     /locus_tag="ACHKCL_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5763170.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11266..11634
                     /locus_tag="ACHKCL_002208"
     CDS             11266..11634
                     /locus_tag="ACHKCL_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5763171.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11728..12381
                     /gene="nfsB"
                     /locus_tag="ACHKCL_002209"
     CDS             11728..12381
                     /gene="nfsB"
                     /locus_tag="ACHKCL_002209"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5763172.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12489..13736
                     /gene="ybdG"
                     /locus_tag="ACHKCL_002210"
     CDS             12489..13736
                     /gene="ybdG"
                     /locus_tag="ACHKCL_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5763173.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13804..15180)
                     /gene="pheP"
                     /locus_tag="ACHKCL_002211"
     CDS             complement(13804..15180)
                     /gene="pheP"
                     /locus_tag="ACHKCL_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5763174.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15282..18419)
                     /gene="cusA"
                     /locus_tag="ACHKCL_002212"
     CDS             complement(15282..18419)
                     /gene="cusA"
                     /locus_tag="ACHKCL_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5763175.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18431..19654)
                     /gene="cusB"
                     /locus_tag="ACHKCL_002213"
     CDS             complement(18431..19654)
                     /gene="cusB"
                     /locus_tag="ACHKCL_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5763176.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19670..20002)
                     /gene="cusF"
                     /locus_tag="ACHKCL_002214"
     CDS             complement(19670..20002)
                     /gene="cusF"
                     /locus_tag="ACHKCL_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5763177.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20026..21408)
                     /gene="cusC"
                     /locus_tag="ACHKCL_002215"
     CDS             complement(20026..21408)
                     /gene="cusC"
                     /locus_tag="ACHKCL_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5763178.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21565..22248
                     /gene="cusR"
                     /locus_tag="ACHKCL_002216"
     CDS             21565..22248
                     /gene="cusR"
                     /locus_tag="ACHKCL_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5763179.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22238..23686
                     /gene="cusS"
                     /locus_tag="ACHKCL_002217"
     CDS             22238..23686
                     /gene="cusS"
                     /locus_tag="ACHKCL_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5763180.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT         5700 a         6494 c         6040 g         5517 t
ORIGIN      
        1 cggcccaatt ttttcaaatg cccctggaga gataatatcc acatgcgcac aatcctgacg
       61 atagatatcc agctccgcaa tccacggcga ccaggcgcgt tcggggctca taccttcctg
      121 aagcgtacgt tcagcaacaa acagcgtcgc ttttccgtca aacggtacgc tatgagcagt
      181 cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
      241 agtacttccc tgctgtgccg ccaggaaggc ctcgcgctcg cggttaatct ccgccagcac
      301 ttccgggtcc agaccattag cttctttttc ctgccagttt tgcgtttctg gcggccaggt
      361 atccagcaag ccaagaaatg ccacctgttc gccacgggca cgtagtcgcg ccgcaatgcc
      421 ctgcgccagt gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg
      481 ttcaagtaac gttgccagat gcgcttcgca gacttcatcc aggttcgtcg ctgtctgcat
      541 ggggccatga gggcgcggcg actgaatgcc gataatcgac catagtggat cgagataacg
      601 cgagagcacg ctaaactgcc aggcaaaacc ggatgccgga tggaaacaaa acagcgtcgg
      661 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg agctgtcctc
      721 ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg
      781 cgtcacctga cgggcaaact gccgacttaa ctgcgctgcc agtttcattg ccagtagcga
      841 atgaccgcca agcgcgaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
      901 cgcgaatgcc gcagcgataa tcgtttcact gcccgctttc ggcgcacgcc ccggcgtttg
      961 tgccttaagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagagg
     1021 taactgtgga agttgcagca gaacgaccgg cgccatatgc ggcggcaatg tttcgcgaag
     1081 ctgtgcctgt aatgcgctgg tatccaacgg cagacctgat tgcgacacca gatagcccac
     1141 caactgacgc gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac
     1201 ggcttgttcg acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg
     1261 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc
     1321 cagccagcgg gcaacgtctc cggtacggta catccgttca ccagggacaa aaggatcggc
     1381 aatgaagcgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttgaatacc
     1441 ggtgagatag agatctcccg ccacacccgg cggcaccgga tgcatcatcg catcgagaat
     1501 gcgcaagccc gtattccaca ccggataacc aatcggcaca ctgctgccgc gcacctgtgc
     1561 cagttcctcg ccaaaagccg gataccaact cacatctacc gccgcttccg tcgggccata
     1621 tagattatgc aacggcgcgc ccgttaactg ttgccattcg cggcataaat cagccggtaa
     1681 ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag cggtttgcgg
     1741 cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc
     1801 atattcggca aagaattgct gcatagcgag cgggtcgcgg tgcgcttccg gttcagccat
     1861 caccagttta gccccggcaa taaacggcca gaaaaactcc cacaccgaga catcaaaact
     1921 gcacggcgtt ttttgggcaa cgacatcttc acctgtaagt ggataatgat tttgcatcca
     1981 caacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
     2041 gccagaggta aagatgatat aagccgtgtg atgcggttgt gaaagttgca gcggcgcact
     2101 gccctgcggt gtaagcgggg cgttatagca aaggttggtt aaatctggaa catcggcaaa
     2161 gcgcggcagt tgatcgtcgg tggtgattaa cagcgacgga cgcgcatctt ccagcatcat
     2221 tttcaggcga tcgtccggat aaccggtatc cagcggtaac caggccgcac ctgcttcaac
     2281 aatcgcatgt agcgccaggg tcaaaaagac cgagcgcggt aatgccaccg ccacgctgtc
     2341 ccctggttta acgccgtgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
     2401 ttcccgatag ctgaactggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
     2461 cgcttgttct gccaccagcg cgctaagcgt ggtttctgga atctcaacct gagtggcgtt
     2521 gatctgcgcc agctgcgcat actcacctgg cagcataata tcaacatcgc cgcacaacag
     2581 agccggatcc gcagcgaatt gggcgatcag cattttcagg cgttcagcat gctggattaa
     2641 cgttggctca tcgtaatgct gtttattggc gaggatctca atactcaaat caccgtgttc
     2701 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg
     2761 cgcctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc
     2821 aaacagcggt tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta
     2881 acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag
     2941 cgtttcttgt gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg gtccggtagc
     3001 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcgt agtccatgcg
     3061 attgcacaat cgccccagcc aaaaggctgc cagcgcaagg gctaaatcgg tacgctgcac
     3121 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
     3181 tagaatatct gccgaagcgc tgcgccccgc taaaggtgcc ggagaaagtg acgcgggcgg
     3241 cggcagttga cgacgctgtt ccgcccagaa tgccgcatca cgctgccagg cttcgctttc
     3301 gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt
     3361 ttgttcgcca cgcagcaatg cgcagtaaat gttggcgatc tgccgagtaa tggccgggaa
     3421 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatctgc
     3481 cacctgtatc agctggtgaa agaccagtgg tttaccgcta tcgacgcgca aatcttgttg
     3541 caaatccgcc tgcattaatg cctgcgcagt accgtgcgga tcaatattgg ttcgcaggtc
     3601 gataatttct ggcagttcga atatcagcgc atcatcgacc cattgccaga cttcgccgtt
     3661 atcttccgta aaacgcatcc gcagcgtatc tgcttgcgct aatccggcaa ccaccgcgcg
     3721 ggccagtaat ggcgcatcaa cctctccggt taactcaacg taatgcgcca cgctccaggc
     3781 ggagggtaat tctgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
     3841 taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tcgccggtgt cagagtacgc
     3901 cagtggactt ccagccactg ctggcaggac tcttgtgact gcggctgaca cacaatgtcc
     3961 cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc
     4021 aaaatgtaaa acactccctg tggatcatcg aagggattac tgaatgccat attcaactcc
     4081 tgtcatggaa aagtggttgc cagaggtcga ttaacccctg catcaagcca ccgcgccaac
     4141 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt atagggtgta
     4201 attgcgcata cagcgcctga ttggcccgca tgatcatcgg ctcgcgaata cccgcttcca
     4261 gcacaatgcg cagaccttcg gcgctaactt caccagcttt tagcttttca agtaacacgc
     4321 cctcttgctg cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacagc
     4381 caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc
     4441 cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta
     4501 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
     4561 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg
     4621 tcagcgaaat cagcacaggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca
     4681 aaactgccag cgggcgttct tctgctgtta catcgccggt ggtaaaaatc catacacgac
     4741 gtgaattttt caaccgttca cttttccaga taatttcttt ggctggtatt tctggcgctt
     4801 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
     4861 gccctcgccc gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata
     4921 gttttcgcca gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat
     4981 cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc
     5041 actgccagac atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat
     5101 gatcggtcac accagtgatg tagatccata cgcgctttat cgtcgagtat tcttcagaac
     5161 cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt
     5221 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
     5281 aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg
     5341 ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta
     5401 accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
     5461 ttgatgacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
     5521 aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag
     5581 gcacaagagc cgaccgatac tcctgtttca catgacgata ctattgtcgt taccgccgcc
     5641 gagcagaact tacaggcgcc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac
     5701 cccgttgccc gcgatgtttc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt
     5761 aactccacca gtggtcagcg agggaataac cgacagattg atattcgcgg tatgggtccg
     5821 gaaaacacgc tgattttgat tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag
     5881 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt
     5941 gaacgtattg aagttctgcg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc
     6001 ggcgtggtta acatcattac caaaaaaggc agcggcgagt ggcacggctc ctgggacgca
     6061 tatttcaatg cgccagaaca taaagaggaa ggtgccacca aacgcactaa ctttagcctg
     6121 accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag
     6181 gctgacgcgt gggatatcaa ccagggtcat cagtccgcgc gtgccggaac gtatgccacg
     6241 acgttaccag ccgggcgcga aggggtgatc aataaagata ttaatggcgt ggtgcgctgg
     6301 gacttcgcac cattgcaatc gctggaactg gaagcgggtt acagccgcca gggtaacctg
     6361 tatgcgggcg atacccagaa caccaactct gacgcttaca ctcgctcgaa atatggcgat
     6421 gaaaccaacc gcctgtatcg ccagaactac tcgctgacct ggaacggtgg ctgggataac
     6481 ggcgtgacca ccagcaactg ggtgcagtac gaacacaccc gtaactcgcg tattccggaa
     6541 ggtctggcgg gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat
     6601 aacgatcttg atgacgtgat gctgcatagc gaagttaacc tgccgattga tttcctcgtt
     6661 aaccagacgc tgacgctggg tacggagtgg aatcagcagc ggatgaagga cttaagttcc
     6721 aacacccagg cactgaccgg aacgaatacc ggtggcgcta ttgatggcgt gagtgccacc
     6781 gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caacatggag
     6841 ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc
     6901 aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa
     6961 atgggcattg cccgcgccta taaagcgccg agcctgtacc agactaaccc gaactacatt
     7021 ctctacagta aaggtcaggg ctgctatgcc agcgcgggcg gctgctatct gcaaggtaac
     7081 gatgacctga aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac
     7141 ggctggctgg cgggcatcac ctggttccgt aacgattatc gcaataagat tgaagcaggc
     7201 tatgttgctg tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg
     7261 aaagcggtgg ttgaaggtct ggaaggatcg ttaaacgtac cggttagcga aacggtgatg
     7321 tggactaata acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg
     7381 tcgatcatcc cggagtatac gttgaactca acgctgagct ggcaggcacg agaagatttg
     7441 tcgatgcaaa cgaccttcac ctggtacggt aagcagcagc cgaagaagta caactataaa
     7501 ggtcagccag cggttggacc ggaaaccaaa gaaattagtc cttacagcat tgttgggctg
     7561 agcgcgacgt gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caatctgttc
     7621 gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatttggc aggggccaac
     7681 tatatcgccg gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtatatgagc
     7741 gtaaacactc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc
     7801 ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag
     7861 gaccatgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc atcagtctcg
     7921 aatatggtcg atatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat
     7981 tttgttgagt tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcactac
     8041 gcacaactgc aacacgctgg acgtaaacgt aaaacagagc atttagccgg acggatcgct
     8101 gctgtttatg ccttgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc
     8161 caaccggtgt ggcctgcgga ggtatacggc agtattagcc actgcggggc tacggcattg
     8221 gccgtggttt cccgtcaacc gattggcgtt gatatcgaag aaattttttc tgcgcaaaca
     8281 gcgaccgaat tgacagacaa cattattaca ccagcagaac acgagcgact cgcagactgc
     8341 ggtttagcct tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag
     8401 gcaagtgaga tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa
     8461 cagcaggtca tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa
     8521 aaaatagtca taactctgtg ccaacacgat taattgacaa catctggtac gattcgcccg
     8581 cagccatcac tgactgcggg cgaaagtgta aagcaggtgc cttaccactc tgacctgaca
     8641 accggatatg cggggattgc tccccgcaca acggctactt cttcggttcg taagcgagaa
     8701 cagccttaaa ctcaatatta cgttccttta cggtgaactc acacagcgag tctccgacca
     8761 gaagcgtaaa tcccagcacc gtcaaacaca gcactatgac tgccacaagg gcatatttcg
     8821 tcagcatgtt tatattgcct ccatgcgaag aagggactac catccgagtt gttggggctc
     8881 ggagtggcag ccccaggatt gatagaaata tctcctgggg cttcatcttt ccagacctca
     8941 gagttaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct tccttagcaa
     9001 aatagctgtt tatttataca gtgaatcctg tttcggactt ctgtcggcaa aactcatcat
     9061 tgctcttaaa acgaaatcgc ccgcagccat cactgaccgc gggcgaaagt gtaaagcagg
     9121 tgccttacca tcctgacctg acaaccggat atgcggggat tactccccgc acaacggcta
     9181 cttcttcggt tcgtaagcga gaacagccct gaactcaata ttacgttcct tcaccgtaaa
     9241 ctcacacagc gagtctccga ccagaagcgt aaatcccggt accgtcaaac acagcactat
     9301 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaaggaac
     9361 taccatccaa attgtctggt ttggagtggc agccccaggg ttgatagaaa tatctcctgg
     9421 agcttcatct ttccagacct cagagtaaac ctgaaaccgg aaagcttcag gcacccgccg
     9481 ttatcttacc cttcctcaac aaaatagctg tttatttata cagtaaatcc tgtttcggac
     9541 ttctcactcc tcatttaatc aactgaagct gagcccgttt tttctgcatg cctcaatcgt
     9601 caaaattctt cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa
     9661 ttgcttcagt caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc
     9721 cgctccgact tctatactga atagaaaact ccaacataag agaaacctat gccattaccc
     9781 gattttcatg tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat
     9841 ccgccgggct atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaataag
     9901 atcacggccg gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat
     9961 gtttgccgtg atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg
    10021 caggcagccg cagaccatca tctggaaatt tgcggcggtg gcacgcaccc gtttcagaaa
    10081 tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc
    10141 tatctcatcc agcaggcgac cgtttttggt cagcatgtcc atgttggctg tgccagtggc
    10201 gatgacgcca tttatttgct gcacggcttg tcacggtttg tgccgcactt tatcgccctt
    10261 tccgccgcgt cgccatatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat
    10321 attttttccg cctttcctga taatggcccg atgccgtggg tcagtaactg gcaacaattt
    10381 gaagccctgt ttcgctgtct gagttacacc acgatgatcg acagcattaa agatctgcac
    10441 tgggatattc gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg
    10501 ttaaccctta gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta
    10561 ctgacagaac gcccgttcaa acataaggag aaagattacc tgctgtataa attcaaccgt
    10621 ttccaggcct gccgctatgg gctggaaggc gtcattaccg atccgtacac tggcgatcgt
    10681 cgaccactaa cggaagacac cttgcgattg ctggaaaaaa tcgccccttc tgcacataaa
    10741 attggtgcat cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa
    10801 gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc tgattgggct ggtgaaaaag
    10861 cattgtgaga tctgggccgg tgactaagcc gggaattgcc ttacagaaga atatccctga
    10921 caatggtgtt ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacca
    10981 ccgcagcagg cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac
    11041 tggggcttac gctggcacaa aaactggtga ccacgttcca tctgatggat cttagtcagc
    11101 tttacacttt attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc
    11161 tgcgctttat ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac
    11221 ttcgcggcat actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca
    11281 ctgcacgaaa cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct
    11341 tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga tttttatgct gtcgtcggag
    11401 ctacgcggcg tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag
    11461 caaatatacc caagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg
    11521 tatcccggcg aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat
    11581 ctggtggttg atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga
    11641 aatctatagc gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac
    11701 gcaaaattac tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat
    11761 tccactaagg catttgatgc cagcaaaaaa cttaccccgg aacaggccga gcagatcaaa
    11821 actctcctgc aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc
    11881 agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac
    11941 gaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac
    12001 gatgcctggc tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg
    12061 gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg ccgatatgca ccgtaaagat
    12121 ctgcatgatg atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taatttcctg
    12181 ctcggcgtgg cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc
    12241 ctcgatgcag aatttggtct gaaagagaaa ggctacacca gtctggtggt agttccggtg
    12301 ggtcatcaca gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac
    12361 attaccttaa ccgaagtgta attctccctt gccgggcatc tgcccggcta tttcccctca
    12421 gattctcctg atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag
    12481 gagacgttat gcaggattta atatcccatg ttgaagattt agcgggtatt gagatcgatc
    12541 acaccacctc gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata
    12601 ttattttgca ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc
    12661 tttggttgca aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg
    12721 ggattatcgt caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata
    12781 ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgc
    12841 tgctggatgt tattttgaat ctggcgcaga aattcccggc agcatctcag ttaccgctga
    12901 aagggatatt tcaggggatt aaactgatcg gcgcgattct ggtcggcatt ttgatgatct
    12961 cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg
    13021 tgctgatgtt ggtatttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg
    13081 cgaacgatat gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg
    13141 cggtgatcga tattgggtta accaccgtca aggtgcgtaa ctgggacaat accattacca
    13201 ctattcccac ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat
    13261 ctggcgggcg acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg
    13321 atgaagacga aatgcaacgt ctgaataaag cgcatttgtt aaagccttat ttaaccagcc
    13381 gccatcagga aattaatgag tggaatcgcc agcaaggttc tacggagtcg gtattaaacc
    13441 tgcgccgaat gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc
    13501 atccgcggat tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg
    13561 gtttaccgct cgagatctat gcgtttacca acaccgtggt gtggctggaa tatgaaagca
    13621 ttcaggctga tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc
    13681 atcagtcgcc aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa
    13741 aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat
    13801 gccttatttc cgacgcagcg ttttaaatgc tacaaacagg aatacaatcc acaccggcag
    13861 caggatcgct gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatggcgag
    13921 gaaggcgatg caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg
    13981 cccctgacgt cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt
    14041 caacagcagc gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag
    14101 atagttgatt aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg
    14161 cacgccgcga cggctaacac gggtaaggaa cttcggcgca ttaccctgca cagaaaggcc
    14221 aaacagcatg cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag
    14281 aatgacgaag ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac
    14341 aaacgggcta ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag
    14401 tgaaccgatg taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct
    14461 tttttccgga tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga
    14521 gaacataatt accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc
    14581 gtagcgccag aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca
    14641 caggccaaag ccgatcatac cgatgattgc cagcacttta atcagcgcaa accagaactc
    14701 ggtttcgcca tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc
    14761 ggcagcccaa atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc
    14821 ggtcagctct gccattccca ccagcacgaa catcacccag tagttccagc cggagaggaa
    14881 cccggcgaac ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc
    14941 ttcgaccacc atctcgccga gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc
    15001 gtagcccagc aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag
    15061 accagtacca attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg
    15121 atgaagcgtc ggctcttgat tcgacgcagt atcttccgat acgtttgacg cgtttttcac
    15181 gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt
    15241 cgcaagacac aatccacacg gttaaaccgg gtatcctgct tttatttccg tacccgatgt
    15301 cggtgcagcc acatcagctt atacgctgcc gggataataa acagcgacag caaaggcgcg
    15361 gtgatcatgc cgccaatcat cggcgcggca atccggctca tcacctctga tcctgccccc
    15421 gttccccaca gaatcggcag cagaccagcg ataatcaccg ccaccgtcat cgctttcggg
    15481 cgcacgcgca ggaccgcgcc gtgatgtaac gcctcatcca gcttctgctc gctgaatgtt
    15541 tgcggattat tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc
    15601 acgccaaatt cggcggcgac cccggcaagg gcaataaaac cggtgcccgt cgccacagaa
    15661 agatgaaagc ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg
    15721 ctgataatca gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagatg
    15781 atcatcaacg tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg
    15841 aactgcccgg agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct
    15901 ttttgcaaat cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag
    15961 ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg
    16021 gccacgtctg ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt
    16081 gcctgcggac tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc
    16141 ccttccaccg tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca
    16201 tccgccaccg tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag
    16261 cgcccacctt ccagccgctc ggcaagcgca gaagccacgc ctggcaccgt tcgtgccacc
    16321 tcttcaattt gctccgccat cgcgtcgata tccgccagca cagtgccgga aacttttatg
    16381 ccgatagggc ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc
    16441 cacagattcg ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc
    16501 attgtcatgc ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc
    16561 tccagcggag cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc
    16621 acttcaggta cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct
    16681 gcggaaatcc ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt
    16741 aaaaattccc cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc
    16801 accaacagcg tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga
    16861 atcaaaaagc gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa
    16921 cccatcagga tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt
    16981 ttggtgaacg ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg
    17041 ataaacgaca gcgtgataat cagcagactg ataaacagcg ccggccccac ttcaacagat
    17101 gcattggtaa tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc
    17161 tgccactctt ccagccgttt atgtgcattc tcgatcatga cgatagcggc atcgaccatc
    17221 gccccgacgg caatcgcaat gccacccaac gacataatat tggcattcag cccctggaag
    17281 tgcatgacaa taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc
    17341 gagcgcacat gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc
    17401 aacttgccgc tgaggttgtc gatggcccgg tcaatgagct ggctgcgatc gtatgttgta
    17461 actatctcca cgccttccgg cagactgctt ttcagcgttt ccagtttgtc cttcacggcg
    17521 gcgatcactt cacgggcgtt tttgccggag cgtaggatca ccaccccgcc cacaacttcg
    17581 ccgttcagtt cggcaatgcc ccggcgcatc tccgggccaa cctggatctt cgcaacatca
    17641 cgcagataaa caggcacgcc attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg
    17701 agcgtttgta gatagccgct ggcgcgcacc atatattctg cttccgccag ttcgattgac
    17761 gaaccgcccg cttcctggtt tgaagcatcc agcgcgcttt ttacttcggc gagactgatg
    17821 ccatactgcg ccaggcgctg aggatcgata accacctgat actctttcac cacgccgccc
    17881 accgacgcca cttccgcaac gtcagggatg gtttttagct catatttgag aaaccagtcc
    17941 tgcaatgagc gtaaatcggc cagatcgtgc ttaccgctgc gatccaccag tgcatattca
    18001 tagatccagc caacacccgt ggcatctggt cccagctcgg cgctgactcc cgcaggcagc
    18061 ttaccctgta cctggttgag gtactccagc acccgcgaac gcgcccagta cggatcggta
    18121 ccatcttcga aaatgacata cacataagag tcgccaaact gcgagaaacc acgcacagtc
    18181 ttcgcgccag gcaccgacaa catggtggta gttagcggat aagtcacctg attttcaacg
    18241 atttgcggtg cctgaccggg atagctggtt ttaataatca cctgcacatc ggagagatcc
    18301 ggcaacgcat ccactggcgt gttaatgatg gtccaggtgc cccagatact cagaaataac
    18361 gcgcccatca gcaccaaaaa acggttcgcc accgagcgac gaataatcca ttcaatcatt
    18421 ggttattccc tcaatgcgca tgggtagcac tttcagagcg catccgctcc agtgcgccag
    18481 aaatattggc ttcggaatca atcaggaaca ggccgctgga aaccaccttt tcaccttccg
    18541 ccagaccaga gcgtaatgcg gtgacacctt gcgatgcctg gaaaacggca acgcgttttg
    18601 gtacaaagcg cccgtcggca tcaacggtaa tcacccgctg ttcgctgccg gtatcaatca
    18661 gcgcctgtga cggaatgagc agcatcggtt cgctggcggt attgagttgc aaccaggcgt
    18721 tcattcccgg ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc
    18781 gggtcgcggc atccacgcca ggtagtagcg tccatttgcg gatggtgagt gttttatcag
    18841 gtcgcgccgg aacggtgagc gtaaactgcg aggcatcttt caccagccag gcgatggact
    18901 ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattttcgct accacgttat
    18961 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggtg
    19021 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggcggcga atatccgcct
    19081 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc ggtctgggtc gccgtaccgc
    19141 cggtttcgcg cagcagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
    19201 cgagaagcgg cgcgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
    19261 taaacccggc ggcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
    19321 ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
    19381 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
    19441 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
    19501 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcgac gttttttctg
    19561 caggcggttc cgcctttgca aaccaggtaa aacctgccgc agaaataata ccgcccgcga
    19621 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
    19681 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
    19741 cgccggtttt aattccactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
    19801 ccggccagtt cacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
    19861 caaaaccttt taccacgccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
    19921 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
    19981 cgacttgcag tgcttttttc atttattttc tcctggagtt aaaaattatt gctgccaacc
    20041 gccaccaagt gcggtatata aagaaatttc gttgacctga cgggcataat tcaggtcaag
    20101 tacggtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
    20161 tgcgccgtgc tgatataatg cccgcgcccg ttgcaaagta atttgcagcg atgccagata
    20221 acgctgctgg gcgctaattt gatcgttcag gctttgacgt aatgccagcg catctgccac
    20281 ttctttaaag gcgttctgga ttttctgttc ataattcact accgactgct gctggcgaat
    20341 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
    20401 aggaataaaa ttccacatcc cgctgctggc gttaaataat gacgacagat cgctgctggc
    20461 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcgcgtg cagcaccaat
    20521 attggcatta gccgccatta acgcatgttc agcttccata atatccgggc gctgcaataa
    20581 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgtcgc tgtttactgt
    20641 ctgcgcttgc ggcagcttgc cgtagcttcc cagtaacagt tgcaatgcat tattcgcctg
    20701 cgccagttcc ccctgacgtt tagcgatgtc gctgcgggta ctttcaatca ccccgcgagc
    20761 ctgttccagc gccagaacat tactgctacc tgtcaacagt tgtttttcga caaacgcata
    20821 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
    20881 ctgattgaaa tagctttgcg cgacattaga aaccagcaga atatgcaccg cgcgctgagc
    20941 ttcctcagtg gctaaataat tttgtcgctc ggcttcgctc atgttcttta agcgcccgaa
    21001 aaaatcgagg tcaaagctgg cgttaaggcc agtagaaaac tcccgcgtcg tggctgaatc
    21061 gcctttaaga ttgccgctcc agctgccgct gccctcacca ttgagctgtg ggtagcggtc
    21121 ggcatcagtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
    21181 atcccggtta ttctccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
    21241 ggtgcgccag cccgcgttct gatagttatc tgctgcgtta accaggccgt tctggctgag
    21301 tgagaactgc tgcggtacgg gcattgccgg gcgctgataa tccggtgcca gtgaacaacc
    21361 ggttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
    21421 tctggtgatt ttataccgcc aactttactc gccaggctct gattttccgg tgacaggaaa
    21481 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
    21541 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
    21601 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
    21661 gggctgaatg gctaccatct ggcgatgacc ggagattatg acctgataat cctcgatatt
    21721 atgctgccgg acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
    21781 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
    21841 ttgggggccg atgactatct ggtgaagccg ttcgcgtttg ctgaactgct ggcgcgggtg
    21901 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
    21961 ttgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
    22021 agtaaagagt ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
    22081 tcgcttatcg cctcgcaggt atgggacatg aattttgaca gcgacactaa cgccattgat
    22141 gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
    22201 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
    22261 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca tcgcggcgtt
    22321 tttcgccttt gcatggatca tgatccactc agtaaaagtg cattttgccg agcaggatat
    22381 taatgattta aaagagatta gtgccaccct tgaacgcgta ctaaatcacc ctgacgaaac
    22441 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca atgtgttgat
    22501 ttccctggca gatagccacg gtaaaacggt gtatcactcc cccggtgcgc ctgatatccg
    22561 cgagtttgcg cgcgatgcca tacccgataa agacgcgcgg ggcggcgagg tgtttctcct
    22621 ctccggcccg acgatgatga tgccaggcca cggtcacggg catatggaac acagcaactg
    22681 gcggatgatc agtttgccag ttggcccact ggttgacggc aaaccgattt atacgctcta
    22741 catcgcgctt tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
    22801 tatgaccgca tcggtcatca gcatcctgat cgtctttatc gtgctattgg cggttcataa
    22861 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
    22921 cgacgtccgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
    22981 caaccatatg atcgagcgta ttgaggatgt ctttacccgc cagtccaatt tctcagcgga
    23041 tatcgcccac gaaattcgca caccaattac taatctcata acgcaaacgg aaatcgccct
    23101 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
    23161 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gcccaggccg ataacaacca
    23221 gttaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
    23281 tttcgaggct ttagcggaag atcgcggcgt ggagctgcag tttgttggcg acgagtgcca
    23341 ggttgcgggc gatccgctga tgctgcgtcg ggcgttaagc aacctgcttt cgaacgccct
    23401 gcgttatacg ccacccggag aggctattgt agtgcgctgc cagacggtcg atcatctggt
    23461 gcaagttatc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
    23521 tgaccgtttc taccgcgttg acccctcccg ccaacgaaaa ggcgaaggta gcggcattgg
    23581 gctggcgata gtgaaatcaa ttgttgtcgc gcataaaggc acggttgcgg ttacgtcaaa
    23641 tgcgcggggg acaaggtttg tgattgtgtt gccggagagg gggtgattat taagttcaaa
    23701 ttcagggcga gggtttacgg cgtttttcgc taaatttcca tcattataaa c
//
LOCUS       DAZFKY010000065        23395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFKY010000065 DAZFKY010000000
VERSION     DAZFKY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(44..961)
                     /gene="lpxC"
                     /locus_tag="ACHKCL_002218"
     CDS             complement(44..961)
                     /gene="lpxC"
                     /locus_tag="ACHKCL_002218"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5763181.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(1062..2213)
                     /gene="ftsZ"
                     /locus_tag="ACHKCL_002219"
     CDS             complement(1062..2213)
                     /gene="ftsZ"
                     /locus_tag="ACHKCL_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5763182.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(2274..3536)
                     /gene="ftsA"
                     /locus_tag="ACHKCL_002220"
     CDS             complement(2274..3536)
                     /gene="ftsA"
                     /locus_tag="ACHKCL_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5763183.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(3533..4363)
                     /gene="ftsQ"
                     /locus_tag="ACHKCL_002221"
     CDS             complement(3533..4363)
                     /gene="ftsQ"
                     /locus_tag="ACHKCL_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5763184.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(4365..5285)
                     /gene="ddlB"
                     /locus_tag="ACHKCL_002222"
     CDS             complement(4365..5285)
                     /gene="ddlB"
                     /locus_tag="ACHKCL_002222"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5763185.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(5278..6753)
                     /gene="murC"
                     /locus_tag="ACHKCL_002223"
     CDS             complement(5278..6753)
                     /gene="murC"
                     /locus_tag="ACHKCL_002223"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5763186.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(6807..7874)
                     /gene="murG"
                     /locus_tag="ACHKCL_002224"
     CDS             complement(6807..7874)
                     /gene="murG"
                     /locus_tag="ACHKCL_002224"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5763187.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(7871..9115)
                     /gene="ftsW"
                     /locus_tag="ACHKCL_002225"
     CDS             complement(7871..9115)
                     /gene="ftsW"
                     /locus_tag="ACHKCL_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5763188.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(9115..10431)
                     /gene="murD"
                     /locus_tag="ACHKCL_002226"
     CDS             complement(9115..10431)
                     /gene="murD"
                     /locus_tag="ACHKCL_002226"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5763189.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(10434..11516)
                     /gene="mraY"
                     /locus_tag="ACHKCL_002227"
     CDS             complement(10434..11516)
                     /gene="mraY"
                     /locus_tag="ACHKCL_002227"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5763190.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(11510..12868)
                     /gene="murF"
                     /locus_tag="ACHKCL_002228"
     CDS             complement(11510..12868)
                     /gene="murF"
                     /locus_tag="ACHKCL_002228"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5763191.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(12865..14352)
                     /gene="murE"
                     /locus_tag="ACHKCL_002229"
     CDS             complement(12865..14352)
                     /gene="murE"
                     /locus_tag="ACHKCL_002229"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5763192.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(14339..16105)
                     /gene="ftsI"
                     /locus_tag="ACHKCL_002230"
     CDS             complement(14339..16105)
                     /gene="ftsI"
                     /locus_tag="ACHKCL_002230"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5763193.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(16121..16486)
                     /gene="ftsL"
                     /locus_tag="ACHKCL_002231"
     CDS             complement(16121..16486)
                     /gene="ftsL"
                     /locus_tag="ACHKCL_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5763194.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(16483..17424)
                     /gene="rsmH"
                     /locus_tag="ACHKCL_002232"
     CDS             complement(16483..17424)
                     /gene="rsmH"
                     /locus_tag="ACHKCL_002232"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5763195.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(17426..17884)
                     /gene="mraZ"
                     /locus_tag="ACHKCL_002233"
     CDS             complement(17426..17884)
                     /gene="mraZ"
                     /locus_tag="ACHKCL_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5763196.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(18486..19490)
                     /gene="cra"
                     /locus_tag="ACHKCL_002234"
     CDS             complement(18486..19490)
                     /gene="cra"
                     /locus_tag="ACHKCL_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5763197.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(19572..19658)
                     /locus_tag="ACHKCL_002235"
     CDS             complement(19572..19658)
                     /locus_tag="ACHKCL_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5763198.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(19670..20161)
                     /gene="ilvN"
                     /locus_tag="ACHKCL_002236"
     CDS             complement(19670..20161)
                     /gene="ilvN"
                     /locus_tag="ACHKCL_002236"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5763199.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(20164..21888)
                     /gene="ilvI"
                     /locus_tag="ACHKCL_002237"
     CDS             complement(20164..21888)
                     /gene="ilvI"
                     /locus_tag="ACHKCL_002237"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5763200.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(22206..23150)
                     /gene="leuO"
                     /locus_tag="ACHKCL_002238"
     CDS             complement(22206..23150)
                     /gene="leuO"
                     /locus_tag="ACHKCL_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5763201.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         5701 a         6720 c         5559 g         5415 t
ORIGIN      
        1 gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacggctg
       61 aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg
      121 cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat
      181 aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga
      241 gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat
      301 aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca
      361 gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg
      421 catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa
      481 aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc
      541 catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt
      601 cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga
      661 tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caagcccgcg agagcagcat
      721 tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca
      781 tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc
      841 gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc
      901 cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca
      961 tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag
     1021 tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgc
     1081 aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc
     1141 acgactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag
     1201 cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg
     1261 tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca
     1321 agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct
     1381 accgtttcga actcatccag acgcaggtcg aagcccgctg tgatgttaac cagcacgccg
     1441 cgtgcgccag acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct
     1501 tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca
     1561 gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt
     1621 tcggcgatgc cttgcacagc gcctttgagt acatcgttcg ctgcgccaaa cgcatccagc
     1681 agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag
     1741 tccacatgct tggacagttc agtgatcccc tgctccgcga atgccatacg cttcttgcct
     1801 tcaaagttga agggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact
     1861 tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag
     1921 accatgtctg caccttccag cgccgcacgc agtgcatcgc gatcctcatc tgccgcattg
     1981 cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga
     2041 atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat
     2101 tcaacacctt caatgcgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg
     2161 acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct
     2221 ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac
     2281 tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt
     2341 ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc
     2401 gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg
     2461 ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc
     2521 gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga
     2581 agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcagctc ggtatagcgc
     2641 ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg
     2701 ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga
     2761 actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc
     2821 acgacattgc cagcataagg aattacctta gtgtggcgca atgccccacc ggtataaacg
     2881 gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct
     2941 tccgtcaata ccgaataact tgatgccaga ccggcaaata tcagttggtc aactttcagc
     3001 ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtggca tgtgatcagg
     3061 tgcacttttg cctgcatccg cacgcccgaa agccctaccg gattcttgat cccttcctga
     3121 tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc gcgtacacgc
     3181 accgatttcg cggtatggac gacgttttcc acatcttctt gcgttacttc ttcttcagaa
     3241 ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat
     3301 accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcacg ttgtacgcac
     3361 ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgcga cgggcagctg
     3421 cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc
     3481 ttcgcggtac caatctccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt
     3541 gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc
     3601 ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt
     3661 gcgcctgctg ctgtagaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat
     3721 cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg
     3781 cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc
     3841 gatagccctg caacacttca ttggcgctgc cttccgggcc atacagcatt ggaagcacct
     3901 gcttgctggt gcgatctggc ggcacgctga aggtatttcc ttccgcgtct accatgtgtt
     3961 gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc
     4021 actgctttct gacgctcacc tgcttaatcc acggcagacg ttgttctatt tgcgtctgga
     4081 tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc
     4141 ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca
     4201 ggcgttgcgc atcttccatc cagcccaaca cgacccagcc gctcaccaac actgtcgtta
     4261 aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa
     4321 cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc
     4381 agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc
     4441 aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatcg
     4501 ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc
     4561 agcactaatg cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac
     4621 tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga cggttgaata
     4681 cgtactgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc
     4741 catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga
     4801 gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg
     4861 ataaccggca aacccagagc agaaatttct gctaactgct tatcactcag gcctttttca
     4921 aactctacgc gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga
     4981 agtttgctgc gtagtttatc cattgaaagc gcagatgcca tcactccgct gccggtataa
     5041 ggcaagccca tcagctcgag catcccctgc agcgtaccat cttcaccgcc gcggccgtgt
     5101 agcgcgataa acactttctg aaagcccatc gacttcagtt gcgtcacgtc aacttctttc
     5161 gggtcgacag gatatgcgtc aataccgcct tcacgcagcc cggctaacac cgctgcgcca
     5221 gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca
     5281 gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc
     5341 aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg
     5401 tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcta ttttcccacg
     5461 tccacgaatt gtgcgacaca gcgaacggct gtccgcaccc ggaattggcg cttcgccagc
     5521 cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc
     5581 atacaggtcg cgtgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc
     5641 cggccagcct gcgcgcgccg ctttaatggt ggcgtccact tccgtcgggt ggtggccgta
     5701 gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc
     5761 gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc
     5821 gtcgtcaatg ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg
     5881 acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctggcgca gcagcgtaaa
     5941 gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa
     6001 gccgtaagtt gtggtctgac gtcccactcg cggtaacaat tcgcggatca ccggatcatc
     6061 aacacacatc accgcacgac cgtaaaacgg caggttgtgc agaaaattaa taaaagtctg
     6121 ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac
     6181 aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat
     6241 caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt
     6301 aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gagaccatcg cggtagttgt
     6361 cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag
     6421 catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt
     6481 atcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg
     6541 atggttgaaa taaatcgtcg ctcccagatt cattaactgc tgcgtgaccg gatttggcgc
     6601 taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat
     6661 accggcacca ccgatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat
     6721 ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc
     6781 atacgatgca aaaggcatcg ctacaattac gcccgggcag cccgactcac ttcatttgcc
     6841 actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag
     6901 gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc
     6961 tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg
     7021 tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcacc
     7081 gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca
     7141 tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg
     7201 gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca
     7261 gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccagc
     7321 acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggtag cgccaacaca
     7381 tcggtacgca ccgggttacc caccacttcc gcattgggga aagcacctgg aaacgcctgc
     7441 atcactttgg tggcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt
     7501 tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccaccagg acctgatacg
     7561 tagccgccca taccgagcac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga
     7621 cgccaggcat tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc
     7681 agaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc
     7741 atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag atggtgcgca
     7801 accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt
     7861 ccttgagcac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat
     7921 aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt
     7981 aactgatcag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt
     8041 taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac
     8101 ggtggtcaat ttctaatgct ttacggccaa tcgacatcgc gcgaaaagcg acgaagaata
     8161 ccattaaaag tgccagcacc acaccgacat accccagttc ttcgccgata atggcgaaaa
     8221 taaagtcagt gtgcgcttcc ggcagatact ccagtttttg tacagagtta cctaaacctt
     8281 gtccccacag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc
     8341 caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg
     8401 cgagtatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca
     8461 atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac
     8521 caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca
     8581 ggaagccgcg caggttatta cgtacttcgt cacctttacg caccagatag ttggcgatat
     8641 agcaaaacag cgacagtttt gtcagctcag caggctggat acgcagcaaa ccgagatcga
     8701 tccaacgcga tgccccttta accgagctcc ccactaccag cacgatcatc agcaggatga
     8761 tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg
     8821 taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga
     8881 acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc
     8941 caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt
     9001 cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca
     9061 ggatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc
     9121 taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc
     9181 aaggctggca caggccgggg agagtagaac catatcgccc ggctgaacac gcggagccag
     9241 caagcgcatc gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc
     9301 cagctgcacg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg
     9361 cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc
     9421 gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg ctttcgaatc
     9481 gttaatccaa cgtacgccgt tatgctccag cacaacttca aagcgatgcg gcagaccagt
     9541 gaatgtggtt aacgctttca ggctgctggc acgcggtaac cctgcggcat ctgccagcgc
     9601 cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcattt ctttcacgtt
     9661 cagcactttc tcgcctttca cccgcagcca ggtttcgccc tgctgatgat tcaggtgata
     9721 gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt
     9781 taaggcatca tcagcattga cgacacaaac tttcgcattt tcgtaaatac gcaattttgc
     9841 cgcacggtat tgctgtaaac caaacggata gcgatccata tgatcttcag tcacgttcag
     9901 aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc
     9961 cagtacgtac agttcgcact catcatccag cagcatcaac gcgggcaggc caatattgcc
    10021 acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt
    10081 gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacagaacag
    10141 ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc
    10201 cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag
    10261 actgcccgtg tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg
    10321 cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc
    10381 ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt
    10441 accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca
    10501 atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg
    10561 aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacgga aagcgtttct
    10621 accacgaaca cgccccccat aatcaccagc aggaattcct gacgcagcag tacggcgata
    10681 atgcctaacg caccacctaa cgccagcgaa cctacatcgc ccataaagac ctgcgccgga
    10741 taggtgttaa accacaggaa gcccagccct gccccgacta tcgcggtaca gacaataacc
    10801 agttccccgg cgtgtcgcag atatggtatg tgtaaatagc tggcaaagtt catgttgccg
    10861 gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga
    10921 ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc
    10981 agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc
    11041 tgcgttgcgg gcgtgtcttt gccaacaagg tacagcgcga aggcgacacc cagcgcaatg
    11101 accgacatcc agaaatactt ccaacgagcg atcaaccctt tggtgtcttt acgcactact
    11161 ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac
    11221 cagacgtacg gattggacgg gtaagcccac agcagtacgg agatcacaat cgccgtcagg
    11281 atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg
    11341 ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag caatcatacg cgggcccatc
    11401 cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa
    11461 aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc
    11521 cattctcctg taaagcgcgt actacctctt ccatggcggc actacgtgaa cccttaacta
    11581 aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt
    11641 tctcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca
    11701 cgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagg
    11761 cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca
    11821 tttcagccag tacctgaaca gcggcggtca ttgaaccgac attggcgttg taggagtcgt
    11881 cgagcagcaa ctggttttct gccagttgaa tggggaacaa acggcctgga acagctttca
    11941 gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag
    12001 cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga acatccacgc
    12061 taccggttgg ggtttgcagg gtaaattccg taccgtgcga agtcacatgg atattggtgg
    12121 cggtgaaatc gctgttggcg gcattaggtg agaaacgcca cactttgcgt gagccaatta
    12181 cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg
    12241 gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt
    12301 ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc cgggcgagtc agactcacag
    12361 tccagacaat ttcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg
    12421 gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat
    12481 aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc
    12541 cggaggaacc tgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca
    12601 gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg atatccagcg
    12661 gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcatgag
    12721 catcgaaacg ttcgcctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat
    12781 cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatgtcgg
    12841 taagttggct aagggttacg ctaatcatgc aatcaccccc agcagacgcg ccaccgtgac
    12901 gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcttcat ggcctttgcc
    12961 cgcgaccagc accacatcgt tctctttagc ctgcataacg gcgcaagtca ccgcttcagc
    13021 acggtcttcc atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat
    13081 gatggcacgc ggctcttcgg tacgcggatt atcgtccgtc accaccgcca catcagcaaa
    13141 ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc
    13201 aaagacacac cacagcttgc ccgcacagtg cagacgcgcc gcctgtaagg ctttttccag
    13261 tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac
    13321 ttccatacgt ccgcaaaccg gttgcagacg agcagcggtt ttcagcagat cagccagtgg
    13381 atagcccagc gccagcagcg tcgccagcgc gagcagcagg ttgctgacgt taaaagcgcc
    13441 catcagatgg ctttcaattt cgccatcgcc ccaacttgag ctaaagcgaa tagtcgcacc
    13501 gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat
    13561 atgatcttcc attgataccg caactgcatc cggcagtttt gccagccagc ggcggcctac
    13621 ttcatcgtcg gcattaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt
    13681 cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttagt
    13741 aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga
    13801 aacttccatt gcgcaaaaag tcgcgtcctg atccaccagc cccgccagct catgctgaac
    13861 atcaactgcc gaaccggtgg tattttctgt cgggatcact ttccccagca gaccgttacc
    13921 aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagcagctg
    13981 ggtcgtcgtg gttttgccgt tggtgcccgt tacgcccacg agacgtaaat tgtcagaggg
    14041 ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat
    14101 gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcacctt tcgcctctgc
    14161 aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg
    14221 atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat
    14281 ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt
    14341 acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg
    14401 tcagcgcatc cggctcgatg ttcatggttc gcaatacgcc gcccatgatg gcaccaaaga
    14461 ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa
    14521 ccagcgcgaa gcgcggctga ctcgcaggcg caacacctgc ggtgtaagca atatatttat
    14581 tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcaatacgat
    14641 agcctttaat cgccgccttc acgccgccgc cacccggtag cgccacgctt tccatcatat
    14701 gcaccacagt gcggacaata gattccggga agacacgttc accgggaacc ggggggtcaa
    14761 ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg
    14821 ctaactgtaa cggagttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt
    14881 cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg
    14941 tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta
    15001 acgccagctt ggaaacaccg acgttactcg acttctgtaa tactccggtc agggttaatt
    15061 cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga
    15121 gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa
    15181 ccgttgaacc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg
    15241 tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcaccgg
    15301 tgttgacatc caccagcacg gcgctaccgg actcagcctt gttaaaggcc accgcgttgt
    15361 tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg
    15421 cagcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca
    15481 caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa
    15541 taccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggatggat
    15601 aataacggcg agattcttca cgcagatgaa ttcccggcag tttcagtttt ttgatgtagt
    15661 ccgccatgtc agggtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt
    15721 taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc
    15781 ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt
    15841 ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg
    15901 tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg
    15961 agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc
    16021 cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt
    16081 tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat
    16141 tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc
    16201 ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag
    16261 catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg
    16321 tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc
    16381 caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca
    16441 tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc
    16501 tgcaatacgc agaactgaac tacgggcacg aggattctca gccacctctt cttcgcccgg
    16561 cattaatttg cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt
    16621 catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac
    16681 aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac
    16741 gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg
    16801 caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc
    16861 agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac
    16921 aatggctcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc
    16981 gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc
    17041 catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag
    17101 ttgcggtgaa gagacaccaa gatcgaggag aatgccgtcg atcttgccga taagatcgcg
    17161 ctcggcaacg tattcgccca gcgcggaaaa aggtccgtgg atgatggaga agcgcggatc
    17221 atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc
    17281 ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc
    17341 aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt
    17401 agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt
    17461 ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt
    17521 catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg
    17581 catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc
    17641 tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac
    17701 gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt
    17761 gatgaatgtc aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat
    17821 aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga
    17881 acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt
    17941 acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga
    18001 tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt
    18061 tatgaatagc tgtaagaaaa aaaccctcat cgtcatcacc gttttaaaat gactcactat
    18121 tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt
    18181 ccccggttaa cagtccctgt tgcgtctgtg tagcgcttga tgccagcggg tttgcgcaaa
    18241 agcaaagaaa agtgcttgtt aaatcggcaa ttcatctctg catgtaacaa aataatgcaa
    18301 aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaactgg
    18361 cgggtttaaa agcatttatc aataatagtc aggaatagtc ttatttactt taaggatatt
    18421 gatgtccagt cccgtactct acgcgccaga gtgaaattca cctggcgcgt atttttgttc
    18481 gcagcttagc tacggctgag cacaccgcgg cgatagagat tacgtttaat gcgcgttaaa
    18541 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
    18601 gcgacatcgc ggtgacgttg agccactgcc agcaccgggc actgtaagaa gtcgagcagt
    18661 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttaccatc gcgacgtagc
    18721 gtgacatcca tcaccccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
    18781 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
    18841 gcatacagga aatgcacttc gcgcggatca tctttccagg cagtacggaa accttgttca
    18901 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
    18961 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
    19021 gtaaagtgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
    19081 cagcgttgat aaaaaggatg ctcaggaggc aacgacgtcg aaacaataat ggcatcgacc
    19141 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
    19201 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
    19261 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
    19321 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
    19381 ttttcaacgg ttttgtcact cacacggtat tgcttcgctt tgccgttaat aacatagctt
    19441 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
    19501 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
    19561 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
    19621 aaagcccaac gtgacatgtt gggctgtaaa ttgcgcattg agatcattct caacgcatta
    19681 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
    19741 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
    19801 tataaagcga gggtgtgaca tcaataattt gcccacggaa tatttccgta ttacgtttaa
    19861 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc ctctcaacat
    19921 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtat aattgctttt
    19981 cgatctgctc aagcactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
    20041 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gccacgctgg gaaaaaaggc
    20101 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
    20161 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
    20221 catcgggtag acgtgctcac tgccatcgac ggtgacatca acaaatacca ggcgattatt
    20281 acgcacctgt tcaagcgcct cgctaagttt gctttccagc tcttgcggat gagagatctg
    20341 gatcccgacg tgaccatagg cttccgccag acggacgaag tctggtaaag actgcatata
    20401 agattgtgaa tgacgaccgg aatagatcat gtcctgccac tgcttcacca tccccaaata
    20461 gcggttatta agattaacca ccagcaccgg cagttcatat tgcaacgctg tagacagttc
    20521 ctgaatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcag
    20581 cgccattttg acgcccagtg ccgcaggtaa accaaagccc atagtgccga ggccaccgga
    20641 gttgatccaa cgacggggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
    20701 cccgacatcg gacgtaacgt aagcgccccc cttcgtcaac cgccaaagag tctcgatcac
    20761 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgac gagcgcgcca
    20821 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
    20881 cgacaagagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcggaatatc
    20941 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
    21001 tgggcagtac tttgccagat tgttagtggt gcgatcgtca aagcggacac cgacagcaaa
    21061 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
    21121 gcccagtgcc tgacgatgcg ttgccggaaa cgctcccagc cccatcaatg aggaaacaac
    21181 gggcagattt aacgcctcca ccgtttcttt cagctgttga tggcagcccg ccgtgattgc
    21241 cccaccgcct acgtagacaa ccggtttttt tgccgccacc agcgtttgca gagcgcgctt
    21301 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactccgg
    21361 ccagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
    21421 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
    21481 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
    21541 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
    21601 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
    21661 acccgacgtt accagcacga cgccgacttc cccggtcgcg cgcgccagac catcggccat
    21721 atgcactgcc gcctgctcat gacgaactaa aacatgaccg ataccaccca cggtatgcaa
    21781 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
    21841 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
    21901 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
    21961 agccagagac ataaacgccg ggtaataaga ataaacagga ggaaaggtga aaagaaacga
    22021 taaaatcctc cattacgaag gataaaaatg ataataaatc ggctaaatcc caccacttac
    22081 agcaatttct ctattttttt aattaatcct aaaatagaga gcccacacat tcagaataaa
    22141 attctacact caataaaaaa tgcagaataa accagacatt catgtctgac ctattctgca
    22201 ttcagttagc gtttgcaaat tgagactaat tgctcttcca tccactgatg gcctttatca
    22261 cgcccggcag cttcatgcca ggagagataa caggttctgc tatttagttt taacggcagc
    22321 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
    22381 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
    22441 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
    22501 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccctta
    22561 attgttgggt gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
    22621 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
    22681 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
    22741 atctgctcaa tgtgattata aatctgcgag gtcagaatac tgtctaacgg gctgcaaaca
    22801 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
    22861 tgtactagtt gcaatgcctg acgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
    22921 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
    22981 acagcgttac tgaccgcagg ttgcgacatt cccagaacat gagcagcacg ggtaatgttt
    23041 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
    23101 ggcttgctga actccgccgt ctctggatga tctgtttgta cctctggcat acttaactcc
    23161 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tcgagaatat
    23221 gatttatcat gcatataaaa taagaaaaag gaaaatgagt aaaattagga aaacataaag
    23281 atacgggttc ttataaaaac agaatccatt gccgtggaaa gcgtcattta ccacagcata
    23341 ataatccata atgttcttgt tgcgtaaaca atctaatcat aactaccgcg aatac
//
LOCUS       DAZFKY010000066        22710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFKY010000066 DAZFKY010000000
VERSION     DAZFKY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(24..173)
                     /locus_tag="ACHKCL_002239"
     CDS             complement(24..173)
                     /locus_tag="ACHKCL_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763202.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            482..1621
                     /gene="wza"
                     /locus_tag="ACHKCL_002240"
     CDS             482..1621
                     /gene="wza"
                     /locus_tag="ACHKCL_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5763203.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1627..2070
                     /gene="wzb"
                     /locus_tag="ACHKCL_002241"
     CDS             1627..2070
                     /gene="wzb"
                     /locus_tag="ACHKCL_002241"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5763204.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2073..4235
                     /gene="wzc"
                     /locus_tag="ACHKCL_002242"
     CDS             2073..4235
                     /gene="wzc"
                     /locus_tag="ACHKCL_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5763205.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4328..5167
                     /gene="wcaA"
                     /locus_tag="ACHKCL_002243"
     CDS             4328..5167
                     /gene="wcaA"
                     /locus_tag="ACHKCL_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5763206.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5170..5658
                     /gene="wcaB"
                     /locus_tag="ACHKCL_002244"
     CDS             5170..5658
                     /gene="wcaB"
                     /locus_tag="ACHKCL_002244"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5763207.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5655..6872
                     /gene="wcaC"
                     /locus_tag="ACHKCL_002245"
     CDS             5655..6872
                     /gene="wcaC"
                     /locus_tag="ACHKCL_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5763208.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6847..8064
                     /gene="wcaD"
                     /locus_tag="ACHKCL_002246"
     CDS             6847..8064
                     /gene="wcaD"
                     /locus_tag="ACHKCL_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5763209.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8075..8821
                     /gene="wcaE"
                     /locus_tag="ACHKCL_002247"
     CDS             8075..8821
                     /gene="wcaE"
                     /locus_tag="ACHKCL_002247"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5763210.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8837..9385
                     /gene="wcaF"
                     /locus_tag="ACHKCL_002248"
     CDS             8837..9385
                     /gene="wcaF"
                     /locus_tag="ACHKCL_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5763211.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9414..10535
                     /gene="gmd"
                     /locus_tag="ACHKCL_002249"
     CDS             9414..10535
                     /gene="gmd"
                     /locus_tag="ACHKCL_002249"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5763212.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10538..11503
                     /gene="fcl"
                     /locus_tag="ACHKCL_002250"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10538..11503
                     /gene="fcl"
                     /locus_tag="ACHKCL_002250"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5763213.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11506..11985
                     /gene="gmm"
                     /locus_tag="ACHKCL_002251"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11506..11985
                     /gene="gmm"
                     /locus_tag="ACHKCL_002251"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5763214.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11982..13205
                     /gene="wcaI"
                     /locus_tag="ACHKCL_002252"
     CDS             11982..13205
                     /gene="wcaI"
                     /locus_tag="ACHKCL_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5763215.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13208..14644
                     /gene="cpsB"
                     /locus_tag="ACHKCL_002253"
     CDS             13208..14644
                     /gene="cpsB"
                     /locus_tag="ACHKCL_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5763216.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14837..16207
                     /gene="cpsG"
                     /locus_tag="ACHKCL_002254"
                     /gene_synonym="manB"
     CDS             14837..16207
                     /gene="cpsG"
                     /locus_tag="ACHKCL_002254"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5763217.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16262..17656
                     /gene="wcaJ"
                     /locus_tag="ACHKCL_002255"
     CDS             16262..17656
                     /gene="wcaJ"
                     /locus_tag="ACHKCL_002255"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5763218.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17658..19136
                     /gene="wzxC"
                     /locus_tag="ACHKCL_002256"
     CDS             17658..19136
                     /gene="wzxC"
                     /locus_tag="ACHKCL_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5763219.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19316..20596
                     /gene="wcaK"
                     /locus_tag="ACHKCL_002257"
     CDS             19316..20596
                     /gene="wcaK"
                     /locus_tag="ACHKCL_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5763220.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20593..21813
                     /gene="wcaL"
                     /locus_tag="ACHKCL_002258"
     CDS             20593..21813
                     /gene="wcaL"
                     /locus_tag="ACHKCL_002258"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5763221.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21824..>22710
                     /gene="wcaM"
                     /locus_tag="ACHKCL_002259"
     CDS             21824..>22710
                     /gene="wcaM"
                     /locus_tag="ACHKCL_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="WcaM from Salmonella enterica is essential for
                     biofilm formation on HEp-2 cells and chicken intestinal
                     tissue but not on a plastic surface; may be involved in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5763222.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVA"
BASE COUNT         5486 a         5731 c         6222 g         5271 t
ORIGIN      
        1 tggagacgca atattaaata ttgtcaacct aaagaaactc ctaaaaacca tattgaatga
       61 cacttaatat aattcttaaa aatagccaat tgccgaattg ttaccttgcc tgctattccg
      121 ttagctgtaa cacttcctcc tgcattattg gagagccaat attcaattac cataatgtca
      181 ccttatattc ggaagttaat ttccgcaata accctttctc atcaacgatt gtatgcagag
      241 agggtttgaa aaattaagct aaatcagtgt attggtagct aaaaagccag gggcggtagc
      301 gtgtctggat gcctgaaaga ccagtctgaa ttattctgtc aatagcctgc ggatgaaatc
      361 aattttttta ggactgatgc cagttaaatt tttattcaat taactgcaag gtaaatgtgc
      421 atggcgacat tgctattaat agtgcacagg ataattactc tgccaaagtg ataaataaac
      481 aatgatgaaa tccaaaatga aattgatgcc attattggtg tcagtaacct tgataagcgg
      541 ttgcacagta cttccgggca gcaatatgtc gacgatgggc aaagacgtca tcaaacagca
      601 ggacgctgat ttcgatctcg acaaaatggt gaatgtttat ccgctgaccc cgcgcctgat
      661 tgaccaatta cgcccacgcc cgaatgtagc gcgccccaat atgacgctgg aaagtgagat
      721 cgcgaattac cagtatcgcg tcgggccggg ggacgttctt aatgtcaccg tctgggatca
      781 cccggaactc accacgccag ccggtcagta ccgcagctcc agcgacaccg gcaactgggt
      841 acagcctgac ggcactatgt tttacccgta tatcggcaag gttcacgtag tcgggaaaac
      901 gctcgctgaa atccgcagtg atattaccgg gcgcttagcg acgtacatcg ctgacccgca
      961 ggtggacgtt aatatcgccg ccttccgctc acaaaaggcc tatatctcag gtcaggtgaa
     1021 taaatctggt caacaggcga tcaccaacgt accactgacc attctcgacg ccatcaacgc
     1081 cgcaggtggc ctgaccgaca ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg
     1141 tgaagagcga atttctttgc aggcgctgat gcaaaacggc gacctcaatc agaaccgact
     1201 gctttacccc ggcgatattc tctacgtgcc gcgtaatgat gatctgaaag tgtttgtgat
     1261 gggtgaagtg aagaaacaga gcaccctgaa aatggacttt agcggcatga ccctgaccga
     1321 agccctgggc aatgctgaag gcatcgacat gaccacctcc aacgccagcg gcatctttgt
     1381 cattcgtccg ctgaaaggcg agggcgggcg taacggcaaa attgccaata tctaccagct
     1441 ggatatgtcc gatgccacgt cgctggtgat ggcgacagaa ttccgcctgc aaccttatga
     1501 cgtggtgtat gtcaccaccg ccccggtttc ccgctggaac cgtctgatca atcagttgct
     1561 gccaactatt agcggtgtcc gttacatgac ggatacagcc agcgacattc ataactggta
     1621 atcgtcatgt ttaacaacat cttagttgtc tgtgtcggca atatttgccg ttccccgacg
     1681 gcggaacgct tactgcaacg ttatcacccg gagctgaaag tggagtccgc tggactcggc
     1741 gcgctggtcg gtaagggcgc tgatcctacc gctatcagcg tcgccgcaga acatcaactg
     1801 tctctggaag gccactgtgc acgacaaatc agccgccgcc tgtgtcgcaa ctatgacctg
     1861 attttgacca tggaaaagcg ccatatcgaa cgcttatgcg agatggcacc ggagatgcgc
     1921 ggcaaagtga tgctgtttgg tcactgggat aacgaatgtg aaatccccga tccgtatcgc
     1981 aaaagccggg aaacgtttgc agcggtgtac acattacttg aacggtctgc ccgccagtgg
     2041 gcgcaggcat tgaacgcaga gcaggtataa gaatgacaga aaaagtaaaa caacatgccg
     2101 ctccggtaac gggcagtgat gaaatcgata ttggtcgcct ggtcggcacc gtcattgaag
     2161 cgcgctggtg ggtgattggc atcaccgctg tattcgccct ttgtgccgtg gtttacacct
     2221 tcttcgccac gccgatttat agtgccgacg cactggtaca aatcgagcaa agcagcggca
     2281 attcgttagt gcaggatatc ggatcggcgt tagccaacaa accgcctgca tcggacgccg
     2341 agatccagtt gattcgttcg cgcctggtgc ttggtaaaac ggtggatgat ctcgacctcg
     2401 atattgcggt gagcaaaaac acgttcccga ttttcggtgc gggctgggat cgcctgatgg
     2461 gacgccagaa cgagacggtg aaagtgacta cctttaaccg tccgaaagag atggaggatc
     2521 aggtgtttac gcttaatgtg ctggacaaca aaaactacac cctgagcagc gatggcggct
     2581 ttagcgcccg tgggcaagcg ggccagatac tgaaaaaaga aggcgtcacg ctgatggttg
     2641 aagccattca cgcccgcccg ggcagtgagt ttaccgtcac caaatactcc acgctgggga
     2701 tgatcaatca actgcaaaac agcctgacgg taacggagaa cggcaaagac gcaggcgtac
     2761 tgagcctgac ttataccggt gaagatcgcg aacagatccg cgacattctt aacagcatcg
     2821 cccgtaacta tcaggaacaa aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg
     2881 ctttcctcgc gcaacagtta ccggaagtac gtagccgcct tgatgttgcc gaaaacaaac
     2941 tgaatgcctt ccgtcaggat aaagattctg ttgatctgcc gctggaagcg aaagcggtgc
     3001 tcgattcgat ggtgaacatc gacgcccagt tgaacgaact gacctttaaa gaggcggaaa
     3061 tctccaagct gtacaccaaa gttcaccccg cgtaccgcac gctgctggag aaacgtcagg
     3121 cgctggaaga cgaaaaagcc aaacttaatg gtcgcgtaac ggcgatgccg aaaacccagc
     3181 aggaaattgt ccgtctgacc cgcgatgtcg agtctggtca gcaggtctat atgcaactgc
     3241 tgaataaaga gcaggagctg aaaatcaccg aggccagcac cgtcggcgat gtgcgcattg
     3301 ttgacccggc aatcactcag cctggtgtgc taaaaccgaa gaaagggctg attatccttg
     3361 gggcgattat ccttggcctg atgctctcta tcgtgggggt gctgctgcgc tcgttgttta
     3421 atcgcggcat cgaaagcccg caggtgctgg aagaacacgg tatcagcgtc tatgccagca
     3481 tcccgctgtc ggagtggcag aaagcgcgcg atagcgtcaa aaccatcaaa gggattaaac
     3541 gctataaaca gagccagcta ctggcggtgg ggaatccaac cgatctggcg attgaagcca
     3601 tccgcagcct tcgtaccagt ttgcacttcg cgatgatgca ggcgcagaac aatgtgttga
     3661 tgatgaccgg ggttagcccg tcaatcggta aaacctttgt ctgcgccaac ctggcggcgg
     3721 tgatcagcca gaccaataaa cgcgtgttgt tgatcgactg cgatatgcgc aaaggctaca
     3781 cccatgagct gttgggcact aataacgtta atggcctgtc ggaaattctg attggtcagg
     3841 gcgatattac tacagctgct aaaccgacct ctattgccaa atttgacctg atcccgcgcg
     3901 gtcaggtacc gccaaatcct tctgaactgt tgatgagcga acgctttgcc gaactggtga
     3961 actgggcgag taaaaactac gacctggtgt tgattgatac gccgccgatt ctggcagtga
     4021 ccgatgcggc aattgttggt cgtcatgtcg gaaccacgtt aatggtggcg cgttatgcgg
     4081 tcaacacatt gaaagaagtg gaaaccagtc tgagccgctt tgagcaaaac ggtattccgg
     4141 tgaaaggggt gattctgaac tccatcttcc gccgcgccag cgcgtatcag gattatggct
     4201 attacgaata cgaatataag tcggatgcga aataacgagg cctgcattcg caccgccccg
     4261 taggccggat aaggcgctca cgccgcatcc ggcaagcaaa ccagctcata agccgggagt
     4321 acaacctatg aaagacaatc cgctgatctc aatctatatg ccgacctgga accgccaaca
     4381 actggcgatt cgggcgataa aatcggtctt gcgccaggac tacagcaact gggagatgat
     4441 catcgtggat gattgctcca cctcctggga acaactgcaa cagtacgtca ccgccctcaa
     4501 cgatccgcgt attacctaca ttcacaacga cattaacagc ggggcgtgcg cggtacgtaa
     4561 ccaggcgatt atgctggcgc aaggggaata cattaccggg atcgatgacg atgatgaatg
     4621 gacgcccaac cgcctgagcg tcttcctcgc ccataaacag caactggtca ctcacgcctt
     4681 tttgtacgct aacgattacg tttgccaggg cgaagtttat tcccaaccgg caagcttacc
     4741 actgtatccc aaatcgccgt actcacgccg cctgttctat aaacgcaata tcattggcaa
     4801 tcaggttttt acctgggcat ggcgtttcaa agagtgtttg ttcgataccg aactgaaagc
     4861 ggcacaggat tacgacatct tcctgcggat ggtggtggag tatggcgaac cgtggaaagt
     4921 ggaagaggcg acgcagatcc tgcatatcaa tcacggtgaa atgcagatca cctcgtcacc
     4981 gaaaaaattc tccggatact tccactttta ccgcaagcac aaagacaaat tcgaccgtgc
     5041 cagtaaaaaa tatcagctgt ttaccctcta ccagatccgc aataaacgta tgacctggcg
     5101 cactttgctg acgctgctgt cggtgcgtaa cggcaaacgt ctggctgatg gtattcgggg
     5161 gcgctaaata tgctggaaga tctacgcgcc aacagctgga gtttacgccc gtgctgcatg
     5221 gttcttgcct atcgcgtcgc tcatttttgt tcggtgtggc gcaaaaagaa cgttctcaac
     5281 aatctttggg cggccccgct gctggtgctg tatcgcatta tcaccgaatg ctttttcggt
     5341 tatgaaatcc aggctgccgc gaccattggc cgccgcttta ctatccatca cggttacgcc
     5401 gtagtgatca ataaaaacgt ggtagcgggg gatgatttca ccattcgcca cggcgtcact
     5461 atcggcaatc gtggtgccga taacatggca tgtccacaca ttggcaacgg cgtcgaactc
     5521 ggtgccaacg tcattattct tggtgatatc acgcttggta acaacgtcac cgtgggcgcg
     5581 ggcagcgtgg tgctcgactc tgtcccggac aacgcgctgg tggtgggaga aaaagcgcga
     5641 gtgaaggtaa ttaaatgaat attctgcaat ttaatgtgcg actggcggaa ggcggggcgg
     5701 caggtgtggc gttagatctc caccagcgtg cgctgcaaca ggggctggcg tcacattttg
     5761 tctacggcta cggcaaaggc ggcaaagaga gcgtcagcca tcagaactat ccgcaggtca
     5821 tcaaacatac gccgcggatg accgcaatgg cgaatattgc cctgtttcgt ctgtttaatc
     5881 gcgatctgtt tggcaatttc aatgagttat atcgcaccat tactcgtaca ccgggtccgg
     5941 tggtcctgca ttttcatgtg ctgcacagct actggctaaa tcttaagagc gtggtgcgct
     6001 tttgcgaaaa ggtgaaaaac cacaaaccgg atgtcactct ggtctggacg ctgcacgacc
     6061 actggagcgt taccgggcgc tgcgccttta ccgacggttg tgaaggctgg aaaacgggct
     6121 gccagaaatg cccgacctta aataactatc cgccggtgaa gattgatcgg gcgcaccagc
     6181 tggtggcggg caaacgccag ttattccgtg agatgctggc gctgggctgt cagtttattt
     6241 cccccagcca gcatgtggct gacgctttca atagcctgta cggtccaggg cgttgccgga
     6301 ttatcaataa cggtattgat atggcaaccg aggcgattct ggcggatttg cctccggtgc
     6361 gcgaaactca gggcaagccg aaaatcgcgg tggtagcgca tgacctgcgt tacgacggca
     6421 aaactaacca gcaactggta cgcgagatga tggcgctggg cgacaaaatc gaactgcata
     6481 cctttggtaa gttctcgccg ttcaccgctg gcaacgtggt taatcacggc tttgaaactg
     6541 acaagcgcaa gttgatgagc gcgctcaatc agatggatgc gctggtgttc agttctcgcg
     6601 tcgataacta cccgctgatt ttgtgtgagg cgttatcaat tggcgtgccg gtgattgcca
     6661 cccatagcga cgcggcgcgt gaagtgttgc aaaaatccgg cggtaaaacc gtcagcgaag
     6721 aagaggtgct gcaactggtc cagttaagca aaccggaaat cgcgcaggcg ttatttggta
     6781 ccacgctggc tgagttcagc caacgcagcc gcgccgccta cagtggacaa cagatgctgg
     6841 aggagtatgt caacttctat cagaatctgt agctacctgc tgctgccgct gatctacctg
     6901 ctggttaacg tcaaaatcac ccagcttggc gaaagtttcc ccatcaccat cgtcactttt
     6961 ttacctgtct tgttactgct gtttttagaa cgcatcagcg ttaaaaaatt gatgattgcc
     7021 ttagggattt gcgcgggact cacggcgttt aactacctgt ttggtcagtc gctggatgcc
     7081 agtaaatacg tcacttcaac tatgctgttt gtctatatcg tgatcattat tggtatggtg
     7141 tggagtattc gctttaaaac gatttcgcca cacaaccatc gcaagatatt acgtttcttt
     7201 tatctggtgg tcgggctggt ggtggcgctg gcggcggtgg agatggcgca aattatcctc
     7261 accggcggca gcagtattat ggagtcgatt tcgaaatatc tgatttacag caacagctat
     7321 gtgctgaatt tcatcaaatt cggcggcaag cgcacgacag cactttattt cgaaccggca
     7381 tttttcgctc tggcattaat ctcaatttgg ctcagcatca aacagtttgg tatcaaaacg
     7441 cctaaaacag atgctatgat tctcgcaggg ataatattat ccggatcgtt ttcaggggtt
     7501 atgaccttta tcctgtttta tttgctggag tgggcatttc aatatctgaa taaagaggcg
     7561 attaagaaaa agttaccgtt agcattgatt tctctggcag tattcctggt tggtgtggta
     7621 attgcatttc cttatatttc cacccgtctg ggcgatttag gtacggaagg atcgtcatcg
     7681 tattatcgta ttgtcggtcc gctggtgatg gtcggttatt ctttgaccca tattgacggt
     7741 gtagtcagat ttggctcact ttatgaatat gtcgcatcat tcggaatatt taacggtgcg
     7801 gatgtcggaa aaaccataga caatggtttg tatctgctga ttatttattt ttcttggttc
     7861 gcggtgtttt tatcgctgtg gtacatgggg aaagtgataa aaatgatgat caacgctttt
     7921 ggtgataacc gcaattttcg cgtgcaatta tatcttttta ctccggtatc gctgtttttt
     7981 accggttcga tatttagccc ggaatatgca tttttaatcg tctgtccgtt tattttgcga
     8041 aaagcgttaa atattacgag gtaagaataa gaacatgttg cttagcataa tcactgtcgc
     8101 gtttcgtaac ctcgaaggga tagtcaaaac acatgcctcg ctggcgcatc tggcgcaggc
     8161 ggaagatatc agcttcgaat ggattgttgt cgatggcggt tccaatgacg gcactcgtga
     8221 gtacctggaa aatctcaatg gtatctataa cctacgcttt gtcagcgagc cagataacgg
     8281 tatctacgac gccatgaaca agggcattgc gatggcacaa ggcaagttcg cgttgtttct
     8341 caactcgggc gatatttttc atcaggatgc cgcatatttt gtccgtaagt taaaaatgca
     8401 aaaagataac gtgatgatca ccggcgatgc gctgctggat tttggcgacg ggcataaaat
     8461 taaacgtagc gccaaaccgg gctggtatat ttatcacagc ctgcccgcca gtcatcaggc
     8521 gatatttttc ccggtatccg gtttgaaaaa atggcgttat gacctggaat ataaagtttc
     8581 ttctgattac gcgctggcag ccaaaatgta taaagcaggt tatgcattta aaaaactcaa
     8641 tggcctggtg tctgaatttt ctatgggtgg ggtatctacc accaataata tggaattgtg
     8701 tgctgacgcg aaaaaagtcc aacggcaaat attacatgtg cctggctttt gggctgaatt
     8761 atcctggcat ttacgccagc gtactacctc aaagacgaaa gccttatata acaaaagctg
     8821 aatataaggg aaaacgatgc aggatttaag cggtttctcg gtgccgaaag ggttccgggg
     8881 cggcaacgct attaaagtgc aattgtggtg ggcagtacag gcaacaatat ttgcctggtc
     8941 gccacaagta ttgtatcgct ggcgggcatt tttattacgt ttattcggcg caaaaatagg
     9001 aaaaaacgta gttattcgtc cgtcagtaaa aattacctat ccgtggaaat taaccttagg
     9061 tgattacgtg tgggtcggcg atgacgtcaa tttatatacc ctcggtgaaa taaccattgg
     9121 cgcacattcg gtgatatcgc aaaaaagtta tttatgcacc gggagccacg accatgcaag
     9181 tcaacatttc accattaacg ccacgcctat tgtgattggc gagaaatgct ggctggcaac
     9241 cgatgttttt gttgccccag gcgtcacgat tggcgacggc accgtcgtgg gtgcgcgaag
     9301 cagtgttttt aaatcgcttc cggcaaatgt ggtttgccgg gggaatcccg cagtggtgat
     9361 acgcaaacgc gttgaaactg aataaattca aaaaaaatac agaggaataa gacatgtcaa
     9421 aagtcgctct catcaccggt gtaaccggac aagacggttc ttacctggca gagtttctgc
     9481 tggaaaaagg ttacgaggtg catggtatta agcgtcgcgc atcgtcattc aacaccgagc
     9541 gcgtggatca catttatcag gatccgcaca cctgcaaccc gaaattccat ctgcattatg
     9601 gcgacctgag tgatacctcc aacctgacgc gtattttgcg tgaagtgcag ccggatgaag
     9661 tgtacaacct gggcgcaatg agccacgttg cggtttcttt tgagtcaccg gaatataccg
     9721 ctgacgtcga cgcgatgggt acgctgcgcc tgctggaggc gatccgcttc ctcggtctgg
     9781 aaaagaaaac ccgtttctat caggcttcca cctctgaact gtatggtctg gtgcaggaaa
     9841 ttccgcagaa agagaccacg ccgttctatc cgcgctctcc gtatgcggtc gccaaactgt
     9901 atgcctactg gatcaccgtc aactaccgtg aatcctacgg catgtatgcc tgtaacggta
     9961 ttctcttcaa ccatgaatcc ccgcgccgcg gcgaaacctt cgttacccgc aaaatcaccc
    10021 gcgcaatcgc caacatcgcc caggggctgg agtcgtgcct gtacctcggc aatatggatt
    10081 ccctgcgtga ctggggccat gccaaagact acgtaaaaat gcagtggatg atgctgcaac
    10141 aggaacagcc agaagatttc gttatcgcga ccggcgttca gtactccgta cgtcagttcg
    10201 tagaaatggc ggcagcacag ctgggcatca aactgcgctt tgaaggcacg ggcgttgaag
    10261 agaagggcat tgtggtttcc gtcaccgggc atgacgcgcc gggtgttaaa ccgggtgatg
    10321 tgattatcgc cgttgatccg cgttacttcc gtccggctga agttgaaacg ctgctcggcg
    10381 acccgaccaa agcgcacgaa aaactgggct ggaaaccgga aatcaccctc agagagatgg
    10441 tgtctgaaat ggtggctaat gacctcgaag cggcgaaaaa acactctctg ctgaaatctc
    10501 acggctacga cgtggcgatc gcgctggagt cataagtatg agtagacaac gcatttttat
    10561 tgctggccat cgcgggatgg tcggttccgc catcaggcgg cagctcgaac agcgcggtga
    10621 tgtggaactg gtattacgca cccgcgacga gctgaacctg ctggacagcc gcgccgtgca
    10681 tgatttcttt gccagcgaac gtattgacca ggtctatctg gcggccgcga aagtgggcgg
    10741 cattgttgcc aacaacacgt atccggcgga tttcatctat cagaacatga tgattgagag
    10801 caacatcatt cacgccgcgc atcagaacga cgtgaacaaa ctgctgtttc tcggatcgtc
    10861 ctgcatctac ccgaaactgg caaaacagcc gatggcagaa agcgagttat tgcagggcac
    10921 gctggagccg accaacgagc cttacgccat tgccaaaatc gccgggatca aactgtgcga
    10981 atcatacaac cgccagtacg gacgcgatta ccgctcagtc atgccgacca acctgtatgg
    11041 cccgcatgac aacttccacc cgagtaattc gcatgtgatc cctgcattgc tgcgtcgctt
    11101 ccacgaggcg acggcacaga atgcaccgga cgtggtggtg tggggcagcg gtacgccgat
    11161 gcgagaattt ctgcacgtcg atgatatggc ggcggcgagc attcatgtca tggagctggc
    11221 gcatgaagtc tggctggaga acacccagcc gatgctgtcg cacattaacg tcggcacggg
    11281 cgttgactgc actatccgcg agctggcgca aaccatcgcc aaagtggtgg gttacaaagg
    11341 tcgggtggtt tttgatgcca gcaaaccgga tggtacgccg cgcaaactgc tggatgtgac
    11401 gcgcctgcat cagcttggct ggtatcacga aatctcactg gaagcggggc ttgccagcac
    11461 ttaccagtgg ttccttgaga atcaagaccg ctttcggggg taatgatgtt tttacgtcag
    11521 gaagactttg ccacggtagt gcgctccaca ccgcttgtct ctctcgactt tattgtcgag
    11581 aacagtcgcg gcgagtttct gcttggcaaa agaaccaacc gcccggcgca gggttactgg
    11641 tttgtgccgg gagggcgcgt gcagaaagac gaaacgctgg aagccgcatt tgagcggctg
    11701 acgatggcgg aactggggct gcgtctgccg ataacagcag gccagtttta cggtgtctgg
    11761 cagcactttt atgacgataa cttctccggc acggatttca ccactcacta tgtggtgctc
    11821 ggttttcgct tcagagtagc ggaagaagaa ctgttactgc cggatgagca gcatgacgat
    11881 taccgctggc tgacgccgga cgcgctgctc gccagtaacg atgttcacgc taacagccgc
    11941 gcctattttc tcgctgagaa gcgtgccgga gtacccggat tatgaaaata ctggtctacg
    12001 gcattaacta ctcgccggag ttaaccggca tcggcaaata caccggcgag atggtggaat
    12061 ggctggcggc gcaaggtcat gaggtgcggg ttattaccgc accgccttac tacccgcagt
    12121 ggcaagtggg cgagaactat tccgcctggc ggtacaaacg agaagagggg gccgctacgg
    12181 tgtggcgctg cccgctgtac gtgccaaaac agccgagcac cctgaaacgc ctgttgcatc
    12241 tcggcagttt tgccgtcagc agttttttcc cgttgatggc gcaacgtcgc tggaagccgg
    12301 atcgcattat cggcgtagtg ccaacgctgt tttgcacgcc gggaatgcgc ctgctggcga
    12361 aactctctgg cgcgcgtacc gtgctgcata ttcaggatta cgaagtagat gccatgctgg
    12421 ggctgggcct tgccggaaaa ggcaaaggcg gcaaagtggc acagctggca acggcgttcg
    12481 aacgtagcgg actgcataac gtcgataacg tttccacgat ttcgcgttcg atgatgaata
    12541 aagccatcga aaaaggcgtg gcggcggaaa acgtcatctt cttccccaac tggtcggaaa
    12601 tcgcccgttt tcagcatgtt gcagacgccg atgttgatgc ccttcgtaac cagcttggcc
    12661 tgccggataa caaaaaaatc attctttact ccggcaatat tggtgaaaag caggggctgg
    12721 aaaacgttat tgaagcagcc gatcgcctgc gcgatgaacc gctgattttt gccattgtcg
    12781 ggcagggcgg cggcaaagcg cggctggaaa aaatggcgca gcagcgtgga ctgcgcaaca
    12841 tgcaattttt cccgctgcaa tcgtatgacg ctttacccgc actgctgaag atgggcgatt
    12901 gccatctggt ggtgcaaaaa cgcggcgcgg cagatgccgt attgccgtcg aaactgacca
    12961 atattctggc agtaggcggt aacgcggtga ttactgctga agcccacaca gaactgggac
    13021 agctttgcga aacctttccg ggcattgcgg tttgcgtaga accggaatcg gttgaggcgc
    13081 tggtggcggg gattcgtcag gcgctcctgc tgcccaaaca caacacggtg gcacgtgaat
    13141 atgccgaacg cacgctcgat aaagagaacg tgttacgtca atttataaat gatattcggg
    13201 gataattatg gcgcagtcga aactctatcc agttgtgatg gcaggtggct ccggtagccg
    13261 cttatggccg ctttcccgcg tactttatcc caagcagttt ttatgcctga aaggcgatct
    13321 caccatgctg caaaccacca tctgccgcct gaacggcgtg gagtgcgaaa gccctgtggt
    13381 gatttgcaat gagcagcacc gctttattgt tgcggaacaa ctgcgtcaac tgaacaaact
    13441 cactgagaac attattctcg aaccggcagg gcgtaacaca gcaccggcca ttgcgctggc
    13501 ggcgctggcg gcaaaacgtc atagcccgga gaacgaccca ttaatgctgg tgctggcggc
    13561 ggatcatgtg attgccgatg aagacgcatt ccgcgccgcc gtgcgtaacg ctatgccgta
    13621 tgccgaagcg ggcaagctgg tgaccttcgg cattgtgccg gatctacctg aaaccggata
    13681 tggctatatt cgtcgcggtg aagtgtcggc gggtgagcag gatacggtgg cctttgaagt
    13741 ggcgcagttt gtcgaaaaac caaatctgga aaccgcgcag gcctatgtgg caagcggcga
    13801 atattactgg aacagcggta tgttcctgtt ccgtgccgga cgctatctcg aagaactgaa
    13861 aaaatatcgc ccggatattc tcgatgcctg tgaaaaagcg atgagcgccg tcgatccgga
    13921 tctcgatttt attcgcgtgg atgaagacgc gtttctggcc tgcccggaag agtcggtgga
    13981 ttacgcggtc atggaacgta cggcagatgc cgttgtggtg ccgatggatg cgggctggag
    14041 cgatgtcggc tcctggtcct cattatggga gatcagcgcc cacaccgccg agggcaacgt
    14101 ttgccacggc gatgtgatta atcacaaaac tgaaaacagc tatgtgtatg ccgaatctgg
    14161 cctggtcacc accgtcgggg tgaaagattt ggtggtagtg cagaccaaag atgcggtgct
    14221 gattgccgac cgtaacgcgg tgcaggatgt gaaaaaagtg gtcgagcaga tcaaagccga
    14281 tggtcgccat gagcatcggg tgcatcgcga agtctatcgc ccgtggggca aatatgactc
    14341 tatcgacgcg ggcgaccgct accaggtgaa acgcatcacc gtgaaaccgg gcgaaggctt
    14401 gtcggtacag atgcaccatc accgcgcgga acactgggtg gttgtcgcgg gaacggcaaa
    14461 agtcaccatt gacggtgata tcaaactgct tggtgaaaac gagtcgattt atattccgct
    14521 gggggcgacg cactgcctgg aaaacccggg gaaaattccg ctcgatttaa ttgaagtgcg
    14581 ctccggctct tatctcgaag aggatgatgt ggtgcgcttc gcggatcgct acggacgggt
    14641 gtaaacgtcg catcaggcgc tgattgtcgg atgcggcgtg aacgccttat ccgacctacg
    14701 gttcggtttt gtaggcctga taagacgcgg cagcgtcgca tcaggcaatg aatgcgaaac
    14761 cgcggtgtaa ataacgacaa aaacaaaatt ggccgtttcg gtcagggcca actattgcct
    14821 gaaaaagggt aacgatatga aaaaattaac ctgctttaaa gcctatgata ttcgcgggaa
    14881 attaggcgaa gaactgaatg aagatattgc ctggcgcatt ggtcgtgcct atggcgaatt
    14941 tctcaaaccg aaaaccattg tgttaggcgg tgatgcccgt ctcaccagcg aaaccttaaa
    15001 actggcgctg gcaaaaggtt tacaggatgc gggcgttgac gtgctggata ttggtatgtc
    15061 cggcaccgaa gagatctatt tcgccacgtt ccatctcggc gtggatggcg gcattgaagt
    15121 tactgccagc cataacccga tggattacaa cggcatgaaa ctggtgcgcg aaggggctcg
    15181 cccgatcagc ggcgataccg gactgcgcga tgtccagcgt ctggcagaag ccaacgactt
    15241 tcctcccgtt gatgaaacca aacgcggtcg ctatcagcaa atcaatctgc gtgacgctta
    15301 cgttgatcac ctgttcggtt atatcaacgt caaaaacctt acgccgctca agctggtgat
    15361 caactccggg aacggcgcgg cgggtccggt ggtggacgcc atcgaagccc gctttaaagc
    15421 cctcggcgca ccggtggaat tgatcaaagt gcacaacacg cccgacggca atttccccaa
    15481 cggtattcct aacccgctgc tgccggaatg tcgcgacgac acccgcaatg cggtcatcaa
    15541 acacggcgcg gatatgggca ttgcttttga tggcgatttt gaccgctgtt tcctgtttga
    15601 cgaaaaaggg cagtttatcg agggctacta cattgtcggt ctgctggcag aagcgttcct
    15661 cgaaaaaaat cccggcgcga agatcatcca cgatccacgt ctctcctgga ataccgttga
    15721 tgtggtgact gccgcaggcg gcaccccggt aatgtcgaaa accggacacg cctttattaa
    15781 agaacggatg cgcaaggaag acgccatcta cggtggcgaa atgagcgccc accattactt
    15841 ccgtgatttt gcttactgcg acagcggcat gatcccgtgg ctgctggtcg ccgaactggt
    15901 gtgcctgaaa ggaaagacgc tgggcgaact ggtgcgcgac cggatggcag cgtttccgtc
    15961 aagcggtgag atcaacagca aactggcgca acccgttgag gcgattaacc gcgtggaaca
    16021 gcattttagc cgtgaggcgc tggcggtgga tcgcaccgat ggcatcagca tgacctttgc
    16081 cgactggcgc tttaatctgc gctcctccaa caccgaaccg gtggtgcggt tgaatgtgga
    16141 atcacgcggt gatgtgccgc tgatggaagc gcgaacgcga actctgctga cgttgctgaa
    16201 cgagtaatgt cggatcttcc cttaccccat tgcgggtaag gggctaataa caggaacaac
    16261 gatgacaaat ctaaaaaagc gcgagcgagc gaaaaccaat gcatcgttaa tctctatggt
    16321 gcaacgcttt tcagatatca ccatcatgtt tgccggacta tggctggttt gcgaagtgag
    16381 cggactgtca ttcctctaca tgcacctgtt ggtggcgctg attacgctgg tggtgttcca
    16441 gatgctgggc ggcatcaccg atttttatcg ctcatggcgc ggtgttcggg cagcgacaga
    16501 atttgccctg ttgctacaaa actggacctt aagcgtgatt ttcagcgccg gactggtggc
    16561 gttcaacaat gatttcgaca cgcaactgaa aatctggctg gcgtggtatg ggctgaccag
    16621 tatcggactg gtggtttgcc gttcatgtat tcgcattggg gcgggctggc tgcgtaatca
    16681 tggctataac aagcgcatgg tcgcggtggc gggggattta gccgccgggc aaatgctgat
    16741 ggagagcttc cgtaaccagc cgtggttagg gtttgaagtg gtgggcgttt accacgaccc
    16801 gaaactgggc ggcgtttcta acgactgggc gggtaacctg caacagctgg tcgaggatgc
    16861 taaagcaggc aagattcata acgtctatat cgcgatgcaa atgtgcgacg gcgcgcgagt
    16921 gaaaaaactg gtccatcaac tggcggacac cacctgttcg gtgctgctga tccccgatgt
    16981 ctttaccttc aacattctcc attcacgtct tgaagagatg aacggcgttc cggtggtgcc
    17041 gctttacgac acgccgcttt ccggggttaa ccgcctgctc aaacgtgcgg aagacattgt
    17101 gctggcgacg cttatcctgc tgctgatctc cccggtgctg tgctgcattg cgctggcggt
    17161 gaaactcagt tcacctgggc cggttatttt ccgccagact cgctacggca tggatggcaa
    17221 gccgatcaaa gtgtggaagt tccgttccat gaaagtgatg gagaacgaca aagtggtgac
    17281 ccaggcgacg cagaacgatc cgcgcgtcac caaagtgggg aactttctgc gccgcacctc
    17341 gctggatgaa ttgccgcagt ttatcaatgt gctgaccggg gggatgtcga ttgtcggtcc
    17401 acgtccgcac gcggtggcgc ataacgaaca gtatcgacag ctcattgaag gctacatgct
    17461 gcgtcataag gtgaaaccgg gcattaccgg ctgggcgcag attaacggct ggcgcggcga
    17521 aaccgacacg ctggagaaaa tggaaaaacg cgtcgagttc gaccttgagt acatccgcga
    17581 atggagcgtc tggttcgata tcaaaatcgt tttcctgacg gtattcaaag gattcgttaa
    17641 caaagcggca tattgatatg agcttacgtg aaaaaaccat cagcggcgcg aagtggtcgg
    17701 cgattgccac ggtgatcatc atcggcctcg ggctggtgca gatgaccgtg ctggcgcgga
    17761 ttatcgacaa ccaccagttc ggtttgctta ccgtgtcgct ggtgatcatc gcgctggcag
    17821 atacgctttc tgacttcggt attgctaact cgattattca gcgaaaagaa atcagccacc
    17881 ttgaactcac cacgctgtac tggctgaacg ttgggctggg gcttgtggtg tgtgtggcgg
    17941 tgtttttgtt gagtgatctc attggtgacg tgctgaacaa cccggacctg gcaccgttga
    18001 ttaaaacatt gtcgctggcg tttgtggtta tcccccacgg gcaacagttc cgcgcgttga
    18061 tgcaaaagga gctggagttc aacaaaatcg gcatgatcga aaccagcgcg gtactggcgg
    18121 gcttcacttt tacggtggtt agcgcccatt tctggccgct ggcgatgacc gcgatcctcg
    18181 gttatctggt caatagtgcg gtgagaacgc tgctgtttgg ctactttggc cgcaaaattt
    18241 atcgccccgg tctgcatttc tcgctggcgt ctgttgcgcc gaacttacgc tttggtgcct
    18301 ggctgacggc ggacagcatc atcaactatc tcaataccaa cctttcaacg ctggtgctgg
    18361 cgcgtattct cggcgcgggc gtggcagggg gatacaacct ggcgtacaac gtggccgttg
    18421 tgccgccgat gaagctgaac ccgatcatca cccgtgtgtt gtttccggca ttcgccaaaa
    18481 ttcaggacga taccgaaaag ctgcgcgtca acttctacaa gctgctgtcg gtggtgggga
    18541 ttatcaactt tccggcgctg ctcgggctga tggtggtgtc aaataacttt gtaccgctgg
    18601 tatttggcga aaagtggaac agcattattc cggtgctgca attgctgtgt gtggtgggtc
    18661 tgctgcgctc cgtaggtaac ccgattggtt cgctgctgat ggcgaaagcg cgggtcgata
    18721 tcagctttaa attcaacgta ttcaaaacat ttctgtttat tccggcgatt gttattggtg
    18781 ggcagatggc gggcgcaatc ggcgtcacgc tcggtttcct gctggtgcag attatcaaca
    18841 ccattctgag ttacttcgtg atgattaaac cggttcttgg ttccagttat cgccagtaca
    18901 tcctgagttt gtggctgccg ttttatctct cgctgccgac gctggtggtc agttatgtgc
    18961 tgggcattgt gctgaaaggg caactggcgc tggggatgct gctggcggtg caaatagccg
    19021 cgggcgtgct ggcgtttgtg gtgatgattg tgctgtcgcg ccatccgctg gtggtggaag
    19081 tgaagcgcca gttttgtcgc agcgaaaaaa tgaaaatgct tttacgggcg gggtgagttg
    19141 ttgttttcgg ttccgacaac ggttgccggg tgcagcgtaa aacaccttat ccatcctacg
    19201 gttctgtgct cgggtcttgt aggcctgata agacgcgcca gcgtcgcatc aggcaaaaaa
    19261 cggcaacgaa cacaacaaac acagcctcac ctggcatcaa atttatgagg aaaaaatgaa
    19321 attacttatt ctgggcaacc acacttgcgg caatcgtggc gacagcgcca tcttgcgcgg
    19381 cttacttgat gccatcaaca tcctcaatcc acataccgaa gtggacgtga tgagccgcta
    19441 tccggtcagt tcttcctggc tgctcaaccg cccggtaatg ggtgatccgc tgttccttca
    19501 aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc gttaaaaaag tcctccgtcg
    19561 ccgctatcag caccaggtat tgctctcacg cgtcaccgac actggcaagc tgcgcaatat
    19621 cgccatcgcc cagggattca ccgacttcgt gcgcctactg tcaggttacg acgccattat
    19681 tcaggtcggc ggatcgtttt ttgtcgatct ctacggcgtg ccgcagtttg aacatgcact
    19741 ttgcacattt atggcgaaaa agccgctgtt tatgattggt cacagcgtcg gtcccttcca
    19801 ggatgagcaa tttaaccaac tggcgaacta cgtttttggt cactgcgacg cgctgatctt
    19861 gcgcgaatcg gtcagcctcg atctaatgaa acgcagcaat atcaccactg caaaagttga
    19921 acatggcgtc gataccgcgt ggctggtcga tcaccacaca gaagacttca ccgccagcta
    19981 tgccgtccaa cactggctgg acgttgccgc acaacagaaa acggtggcaa ttaccctgcg
    20041 cgaactggca ccgttcgaca aacgcctcgg caccactcag caagtgtatg aaaaagcctt
    20101 tgccggggtg gtcaatcgca ttctcgacga aggctatcag gtcattgcgc tctccacctg
    20161 tacgggtatc gacagctata acaaagacga ccgcatggtg gcgctcaacc tgcgccagca
    20221 catcagcgat cctgcccgtt atcacgtagt gatggatgaa cttaacgatc tggaaatggg
    20281 caaaattctc ggtgcctgtg aactcaccgt cggtacgcgc ctgcactccg ccattatctc
    20341 aatgaacttt gctactccgg cgattgccat caactatgaa cataaatccg ccggggttat
    20401 gcagcagctg ggactaccgg agatggcaat tgatatccgt catttattag acggcagcct
    20461 gcaagcgatg gttgcggata ccttaggcca gcttccggcg ctgaacgcac gacttagcga
    20521 agccgtcagt cgtgagcgtc agacgggaat gcagatggtg cagtccgtac tggaacgcat
    20581 cggggaggtg aaatgaaggt cggcttcttc ttattgaaat ttccgctgtc gtcagaaacc
    20641 ttcgttctta accaaatcac tgcgtttatt gatatgggct ttgaagtgga gattgtcgcg
    20701 ctgcaaaaag gcgacacaca aaacacccac gtggcatgga ctaaatacaa ccttgccgcc
    20761 agaacccgct ggttacagga cgaacctacg ggcaaagtgg cgaaactgcg ccaccgagcc
    20821 agtcagacct tgcgcggcat tcatcgtaaa aatacctggc aggcgctcaa cctcaaacgc
    20881 tatggtgccg agtcgtggaa cctgattttg tctgccattt gcggccaggt cgcaacaccg
    20941 tttcgcgccg atgtgttcat cgctcatttt ggccctgcgg gggtaaccgc agcaaaacta
    21001 cgcgaactgg gtgtcattca cggcaaaatt gccactattt tccacggcat tgatatttcc
    21061 agtcgggaag tgctcaacca ttacactccc gaatatcaac aactgtttcg acgtggcgac
    21121 ctgatgctac cgataagcga tctgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg
    21181 gaaaaaatcg ccgtatcgcg catgggcgta gatatgacgc gctttagccc gcgtcccgtg
    21241 aaagcgcccg caacaccgct ggagattatt tccgtcgcac gcttaaccga gaaaaaaggt
    21301 ctgcatgtgg cgattgaagc ctgccggcag ttgaaagagc agggcgtggc atttcgctat
    21361 cgcatcctcg gcattggccc gtgggaacga cgcctgcgca cgctcatcga acaatatcaa
    21421 ctggaagatg tggtggagat gccgggcttt aaaccgagcc atgaagtgaa agcgatgctc
    21481 gacgacgcgg atgtcttcct gttgccatcg gttacaggtg cggatggcga tatggaaggc
    21541 attccggtgg cgctgatgga ggcgatggcg gtcggcattc cggtggtttc tactctgcat
    21601 agcggaatac cggaactggt ggaggctgac aaatccggct ggctggtgcc tgagaacgat
    21661 gctcgcgcac tggcgcaacg actggcggcg tttagccaac tggacaccga cgaattggct
    21721 ccggtcgtca aacgcgcgcg cgaaaaagtt gaacacgatt ttaaccagca ggtgatcaat
    21781 cgagaactcg ccagcttgct gcaggcttta tagaggagaa cgtatgccat ttaaaaaact
    21841 ctcccgacgc accttcctga cggcaagctc ggcgcttgcc ttccttcata cccctttcgc
    21901 tcgcgcactt cccgcccgac aaagcgttaa tatcaacgac tacaaccctc acgactggat
    21961 cgcctcattt aaacaagcct tcagcgaagg gcaaacggtt gtcgtgcctg ctggattcgt
    22021 ttgtgacaat atcaacaccg gcatcttcat tcctcctggc aaaacgttac acatccttgg
    22081 aagcctgcgc ggcaacggca gagggcgatt tgtcttacag gacggcagcc aggtgacagg
    22141 ggaggagggc ggcagtatgc ataacatcac cctggatgtg cgtggttctg actgcaccat
    22201 caaagggctg gcgatgagcg gctttggtcc ggtaacgcag atttatatcg gcggcaaaaa
    22261 caaacgggtc atgcgcaacc tgaccatcga taacctgacc attagccacg ctaattacgc
    22321 catcttacgc cagggatttc ataaccagat tatcggtgcc aacatcacca attgtaagtt
    22381 cagcgactta cagggcgatg ccattgaatg gaacgtggcg attaacgacc gcgatatttt
    22441 gatctccgac catgtcatcg agcgcatcaa ctgtactaac ggcaaaatca actggggcat
    22501 tggcataggc cttgcgggaa gcacttacga taataattac ccggaagatc aggcagtgaa
    22561 aaactttgtc gtggcgaata tcacgggatc ggattgtcgg cagttgatac atgttgaaaa
    22621 tggtaaacat tttgttattc gtaatatcaa agcccgcaat atcacgcagg atttcagtaa
    22681 gaaagcaggt attgataacg ccacagtcgc
//
LOCUS       DAZFKY010000067        22651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFKY010000067 DAZFKY010000000
VERSION     DAZFKY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..602)
                     /gene="yncG"
                     /locus_tag="ACHKCL_002260"
     CDS             complement(<1..602)
                     /gene="yncG"
                     /locus_tag="ACHKCL_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5763223.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILK"
     gene            869..2368
                     /gene="ansP"
                     /locus_tag="ACHKCL_002261"
     CDS             869..2368
                     /gene="ansP"
                     /locus_tag="ACHKCL_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5763224.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2483..3544)
                     /gene="yncE"
                     /locus_tag="ACHKCL_002262"
     CDS             complement(2483..3544)
                     /gene="yncE"
                     /locus_tag="ACHKCL_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5763225.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3786..5888
                     /gene="pqqU"
                     /locus_tag="ACHKCL_002263"
     CDS             3786..5888
                     /gene="pqqU"
                     /locus_tag="ACHKCL_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5763226.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5924..6589)
                     /gene="mcbR"
                     /locus_tag="ACHKCL_002264"
     CDS             complement(5924..6589)
                     /gene="mcbR"
                     /locus_tag="ACHKCL_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5763227.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6787..7824)
                     /gene="curA"
                     /locus_tag="ACHKCL_002265"
     CDS             complement(6787..7824)
                     /gene="curA"
                     /locus_tag="ACHKCL_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5763228.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8005..8523
                     /gene="mddA"
                     /locus_tag="ACHKCL_002266"
     CDS             8005..8523
                     /gene="mddA"
                     /locus_tag="ACHKCL_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5763229.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8520..8969
                     /gene="ydcZ"
                     /locus_tag="ACHKCL_002267"
     CDS             8520..8969
                     /gene="ydcZ"
                     /locus_tag="ACHKCL_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5763230.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8970..9203)
                     /gene="ydcY"
                     /locus_tag="ACHKCL_002268"
     CDS             complement(8970..9203)
                     /gene="ydcY"
                     /locus_tag="ACHKCL_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5763231.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9289..9462)
                     /gene="ortT"
                     /locus_tag="ACHKCL_002269"
     CDS             complement(9289..9462)
                     /gene="ortT"
                     /locus_tag="ACHKCL_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5763232.1"
                     /translation="MSLYQHMLFFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9657..9752
                     /gene="yncL"
                     /locus_tag="ACHKCL_002270"
     CDS             9657..9752
                     /gene="yncL"
                     /locus_tag="ACHKCL_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5763233.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9849..11273)
                     /gene="patD"
                     /locus_tag="ACHKCL_002271"
     CDS             complement(9849..11273)
                     /gene="patD"
                     /locus_tag="ACHKCL_002271"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5763234.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11295..12089)
                     /gene="ydcV"
                     /locus_tag="ACHKCL_002272"
     CDS             complement(11295..12089)
                     /gene="ydcV"
                     /locus_tag="ACHKCL_002272"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5763235.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12079..13020)
                     /gene="ydcU"
                     /locus_tag="ACHKCL_002273"
     CDS             complement(12079..13020)
                     /gene="ydcU"
                     /locus_tag="ACHKCL_002273"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5763236.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVTQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13021..14034)
                     /gene="ydcT"
                     /locus_tag="ACHKCL_002274"
     CDS             complement(13021..14034)
                     /gene="ydcT"
                     /locus_tag="ACHKCL_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5763237.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14052..15197)
                     /gene="ydcS"
                     /locus_tag="ACHKCL_002275"
     CDS             complement(14052..15197)
                     /gene="ydcS"
                     /locus_tag="ACHKCL_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5763238.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15442..16848)
                     /gene="ydcR"
                     /locus_tag="ACHKCL_002276"
     CDS             complement(15442..16848)
                     /gene="ydcR"
                     /locus_tag="ACHKCL_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5763239.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16927..17343)
                     /gene="hicB"
                     /locus_tag="ACHKCL_002277"
     CDS             complement(16927..17343)
                     /gene="hicB"
                     /locus_tag="ACHKCL_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5763240.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17389..17565)
                     /gene="hicA"
                     /locus_tag="ACHKCL_002278"
     CDS             complement(17389..17565)
                     /gene="hicA"
                     /locus_tag="ACHKCL_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO5763241.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17787..18017
                     /gene="yncJ"
                     /locus_tag="ACHKCL_002279"
     CDS             17787..18017
                     /gene="yncJ"
                     /locus_tag="ACHKCL_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO5763242.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18109..20070)
                     /gene="rlhA"
                     /locus_tag="ACHKCL_002280"
     CDS             complement(18109..20070)
                     /gene="rlhA"
                     /locus_tag="ACHKCL_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5763243.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20143..20679)
                     /gene="sutR"
                     /locus_tag="ACHKCL_002281"
     CDS             complement(20143..20679)
                     /gene="sutR"
                     /locus_tag="ACHKCL_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5763244.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20732..21943
                     /gene="ydcO"
                     /locus_tag="ACHKCL_002282"
     CDS             20732..21943
                     /gene="ydcO"
                     /locus_tag="ACHKCL_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5763245.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(21978..22646)
                     /gene="ydcL"
                     /locus_tag="ACHKCL_002283"
     CDS             complement(21978..22646)
                     /gene="ydcL"
                     /locus_tag="ACHKCL_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5763246.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         5486 a         5826 c         5568 g         5771 t
ORIGIN      
        1 ctttttagta tttcttgtaa cgttggtaac tgacatactg catcagcaat cgcactgata
       61 ttcgtggcat tatcctgaaa ccattcatgc ccaggccccc atgtgcgcat agtgcaaaga
      121 taacagtcca ccagcgttag ttgctcacca aacgcataag gttcagcggt caattgtgaa
      181 ttcagccaga cataaagcga tttacgatat tcaataacat tcttctttaa ctgttcaggt
      241 gcgtcagatg cccaacgctc agggtaatcg gcgaaagtga acgttggata gacattagca
      301 accagccaga ccaataaccg ttgaaactgc tgacgttcgg cacgcccaac cggtggcgca
      361 aggtccgggc gacgatcgag aaccatcaat gcaatcgccg ctgtctccgt cataatctcg
      421 tcattttcca gtgccagtgt cggaacctga cacaacgggt tcagggtttt caataactcg
      481 cgtgaagttc cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc
      541 agtgttagca tcagctcact gattgtagag ccccatccgg gtacgccata caccttaatc
      601 atatgcccct cacaatcttt taaagattaa atgtaggaca gtacaaaggt taaccagcaa
      661 aaggatttgt cattttttcg tcagttgtat taatcccctt caggattgta aggggatgtt
      721 ttgcatcgtt tatcgataac gggtaggatg ctgcgccgat agttttatac ccataaagaa
      781 taatggtgat aactatcatc gccaggatga ataaacattg ttcatggcaa cttatatgac
      841 tttttcatta aagcaatcag ggagagcaat gagtaaacac gacaccgaca cttcagatca
      901 acacgccgcg aaacgccgct ggcttaatgc ccacgaagag gggtatcaca aagcgatggg
      961 caatcgccag gtacagatga tcgccattgg cggcgcgatt ggcaccggct tgtttttagg
     1021 tgcaggagcc cgactgcaaa tggcgggtcc agcactggca ctggtttatt taatttgtgg
     1081 cttgttttcg ttttttattc tgcgtgcatt gggtgagctg gtgctacacc gcccttccag
     1141 tggcagtttt gtctcttatg cccgtgagtt tttgggtgag aaagccgctt atgttgctgg
     1201 ctggatgtac ttcatcaact gggcgatgac ggggattgtt gatattaccg ccgtcgctct
     1261 gtatatgcat tactggggtg cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc
     1321 acttaccatc gttggcacca tgaatatgat cggtgtgaaa tggtttgcgg agatggagtt
     1381 ctggtttgcg cttattaaag tgctcgccat tgtgaccttt ttggtcgtgg gtacggtgtt
     1441 cctcggtagt ggtcagccgc tggatggcaa caccactggc tttcatttaa tcaccgataa
     1501 tggcggcttc ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt
     1561 tgcttttgcc tccattgaaa tggtgggtac agctgccgga gaatgtaaag atccgcagac
     1621 catggtgcct aaagcgatta acagtgtgat ttggcgtatt ggcctgtttt acgtcggctc
     1681 cgtggtgttg ctggttatgt tattgccgtg gagcgcgtat caggcggggc aaagtccgtt
     1741 cgtgacgttt ttctctaaac tgggtgtgcc atatatcggc agcattatga acattgtggt
     1801 gctgaccgct gccctctcca gcctgaattc aggtctgtac tgcaccggac gtattctgcg
     1861 ctcaatggcg atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt
     1921 gccgtatgcc gggattctgg cgacactggt tgtgtatgtc gtcggcgtat tcctcaacta
     1981 tctggtgccg tcgcgcgtat ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc
     2041 ttcatgggcg tttatcatcg tgtgccagat gcgcctgcgt aaagcgatta aagaaggcaa
     2101 agcagcggat gtcagtttta aactgcctgg cgcgcccttc acttcctggc tgacattact
     2161 gtttttactg agtgtccttg tgctgatggc gttcgattac ccgaacggga cttatactat
     2221 cgcggcgctg ccgattatcg gtattctgct ggttataggc tggtttggtg tgcgcaaacg
     2281 cgttgctgaa attcacagca ctgcgccagt cgtcgaggaa gatgaagaaa aacaggaaat
     2341 tgtgtttaag cctgaaacgg cgagctaatt gcaaaattgc ccggctacgt gccgggcaaa
     2401 tttagattac tgacgaaggt gtgccgcatg cggcgtgatc aaagtcaggc aagtctctcc
     2461 gttatcaccc tcgccatatc tattacagcg caatacgaat cacatcgtct ggctgggtag
     2521 cttcctgctg tttagtggat ttttgtttca cactgacata cagcgttttg ccatcggcag
     2581 acagcgccag gctgttcgga tgagtcggcg tatcgaacgt tttcaccact ttatagcttt
     2641 tcgcgtcaat cacactgact ttacctgcct gacgatgcgt tacgtaggct tcattacgcg
     2701 ctgggttaaa cagcacagcc agtgattccg gtgccgcaac cttcgccaga atattgccat
     2761 tacgggtatc gaccactaac acttctgccg ctttagaatc ggtgataaat gcacgctgcc
     2821 tggcggtatc aaggctgatg ttgataaaga agtgctcttt gccgtcatcc agcagctttt
     2881 tacggctgag gattttattg tcggcggtgt cgatggtaat caattcgccg tcagcgttag
     2941 tggtgtaaag acgtttgcct ttgctatcca gtgccagacc ggtactcatt ttaccggtgt
     3001 tctggatggc ggttttcagt ttgatattct cgccatcaac gacccaaatc acgctatctt
     3061 taccaatacc actgatgtaa acggtgttcg tggcatcatc agctaccagc tcacgcggtt
     3121 gcagcgggcg cacctcttcc gtgcgcttac gatcatccag caccagacgg cctttcacct
     3181 cgccagtttt ggcatctatc gccgtgaccg cgctgtttac ggtgttacca aaccacaacg
     3241 tctgagtcgt gttattgatg gtggcaccaa acggcttgag atcgttatgg atcgcctgcg
     3301 tcacttccag agtgaccgga tcaagacgat aaaccacgcc gcctttatcc agtttgcggc
     3361 tttgcgaagt ggcgagccac agcgcgtttt cttgctggct ataagccatt tcgtaggcac
     3421 ctttacctac cgctttacgc agcatttctt ctgcggcctg cgtactgaat gatgaagcaa
     3481 caagcaatga acctaacagt aatgaaccac gcaggcgcaa tgaaaacaga tgacgtaaat
     3541 gcatgacgac tccctttgat gaaccgatat ttttgctgct tcacaatgct tcgctaagcg
     3601 taatcatcgt tacgctcttg ttataaatgg gaatggtaat cattatttac tcaaatgtgg
     3661 agcatttctt gccgttacga ctacaattct ctcaattaat taacgtatcg tgccgtttcg
     3721 ttttccataa atttacaatt tcattaccat gacggtgttt tcccattctt aacctggctt
     3781 ttttcatgaa gattgttttc gttcgacaga ccgttttgcc cgccctgctt gtcctttccc
     3841 ccgttgtttt tgccgctgat gaacagacca tgattgtcag tgccgcgccg caggtggttt
     3901 cagaactgga tacccctgca gcagtaagcg tggtggatgg cgaggagatg cgcctggcaa
     3961 caccgcgcat taacttgtcc gaatcactga ccggcgtgcc tggtttgcag gtacaaaacc
     4021 ggcagaacta tgcgcaagat ttacagctgt cgattcgtgg atttggctcc cgctccactt
     4081 acggaattcg cggtattcgc ctgtatgtgg acggtattcc cgctaccatg cccgacgggc
     4141 aagggcaaac atccaacatc gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc
     4201 ccttctctgc cctgtatggc aacgcgtctg gcggtgtaat gaatgtcacc acccagaccg
     4261 ggcaacagcc accaaccatt gaagccagta gttactacgg cagttttggc agctggcgct
     4321 atgggctgaa agcaacgggc gcaacgggag acggcacaca gcctggcgat gttgattaca
     4381 ccgtctcaac cacgcgtttt acgacccacg gctatcgtga ccatagtggc gcacagaaaa
     4441 atttagctaa tgccaaactg ggcgtacgca ttgatgacgc cagcaaatta agcctgattt
     4501 tcaatagcgt ggatatcaaa gcagatgacc caggtgggct aaccaaagca gaatggaaag
     4561 cgaatccgca acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa
     4621 ctcaggctgg gttgcgctat gaacgtagcc tgagttcgcg ggatgatatg agtgtgatga
     4681 tgtatgccgg agagcgagaa acgacccagt accagtcaat acccatggca ccacaactta
     4741 acccgtcaca tgcgggcagc gtgattaccc tgcaacgcca ttaccaggga atagacagcc
     4801 gctggacaca ccgtggtgaa ctgggcgttc cggtcacgtt cactaccggc ctgaactacg
     4861 aaaacatgag tgaaaaccgc aagggctaca ataacttccg cctgaatagc ggcatgccgg
     4921 agtacgggca aaaaggtgag ttgcgtcgcg acgaacgcaa tctgatgtgg aacatcgatc
     4981 cctatttaca gacgcagtgg cagctgagcg aaaaactgtc gctggatgct ggcgtgcgct
     5041 acagctccgt gtggtttgat tccaacgacc attacgttac tccgggtaac ggcgatgaca
     5101 gcggtgatgc cagttatcac aaatggctac cagccggatc gttaaaatat gcaatgaccg
     5161 atgcctggaa tatctatctg gcagccgggc gtggttttga aacgccgacg attaatgagc
     5221 tgtcttatcg cgctgatggg caaagcggta tgaactttgg tttaaaacca tccaccaacg
     5281 atacaattga gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt
     5341 tccagaccga caccgatgat gaaattgttg tcgatagcag tagcggtggg cgtacgactt
     5401 acaaaaatgc tggaaagacc cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg
     5461 cgggagattt tcgcgtaaac gcgtcctgga cctggcttga tgcgacctat cgcagcaatg
     5521 tttgcaacga acaggattgt aacggtaatc ggatgccagg gatcgcccgt aatatgggct
     5581 ttgcgtcgat aggttatgta ccggaagatg gttggtatgc aggcatggaa gcgcgttata
     5641 tgggcgatat tatggcagat gatgaaaata cggccaaagc gccgtcttat actctcgtcg
     5701 gcttattcac cgggtataaa tacaattacc acaatttaac tgtggatttg tttggtcgtg
     5761 tcgataattt attcgataaa gaatacgttg gttctgtcat tgtcaatgag tcaaacgggc
     5821 gatattacga acctgcgccc gggcgaaatt atggtgtcgg cgtgaatatc gcatggcgat
     5881 ttgagtaatt accttgatgc ccggtatttg ccgggcattt actttaacga ttgtattgct
     5941 ggtataaaat agcaatattt tgttgcataa tttcagcaag acaatgtctg catccctctt
     6001 tgtcttttgc ttttaacgca gcgagtaaca gatgatagtt ttctatatgc tcccgtaatt
     6061 ccgctggatt aatcgcttca tagagataat gtaaaccagg tcccatcctg acccacagtt
     6121 gctcaatcat ctcacacagg atgggcatat ttgagcgatg ataaatcgct aagcgaaata
     6181 gcctgtttac attaatgatt tgttccatgt cgcccttttc ttgcgcctgc tgtaatttct
     6241 cgagcaattc ctggagttcc gccaggtctt gcggggtgag gttttcaaca gccagagcaa
     6301 ctgccattaa ttccagttcg tagcggatcc gattgatttc gtctaattga cgtttcccca
     6361 cttccggaac tgtaaatgct tgcgctggtg cgacagaaag cgcattcacc gaaaccagac
     6421 gtaataatgc ttcacgcaca ggtgtaatac tcatacctaa ttgctccgcc agatttttag
     6481 taatcaggcg tgcgccaggt tttagtgcgc caatactaag ctgatgtttc aggtcgttct
     6541 caacctgcag agtcaaactg acatgtttca ttttttccgt tccaggcatt ttaattcctt
     6601 cgcgtgtttt ctgtccaacc ctgcctgcat ggcagaagga attcacctgg tgtattaaag
     6661 tgaataatca taaaccactc acctttgttg ttattagcag cagaacaatg atgtggttag
     6721 ttaattaaag ctgaaaataa gctttttgcc tggattttta tggcggctta cgccgccgtt
     6781 gaaactttaa tcatcacccg ccacgcggat caccactttg ccgaagtttt tacccttcag
     6841 caggccgata aacgtctgtg gcgcattctc taaaccgtca gtaatttctt cgcggtagtg
     6901 gattttatcc tctttcaccc attgccccat ctccctctga aactcatgga tgcggtgacc
     6961 ataatcctga gcgataataa aaccttgcaa gcgaatacgt tttttcagca ctgtagccat
     7021 caacagaggt aaacgatccg gaccgggtgg tagctctgta gcgttatagc tgctcactaa
     7081 tccgcagacg ggaatgcgcg cagatgtatt aagtaacggt agcaccgcat cgaatacctt
     7141 accgcccacg ttttcataat agatatcaat accttttggg cacgctttcg ccagttgttc
     7201 ggcaaaatca tccgcgtggt gatcaagaca aacatcgaag cctaacacct cggtagcatg
     7261 gcggcatttt tccgcgccac cggctacccc caccactctg caacctttaa gtttgccgat
     7321 ttgccccacc gtcgccccca ctggtcctgt cgccgcagct accaccaacg tttcgccctc
     7381 tttaggctga ccgatatcca gtaggcccat ataagcggta aagcctggca tccctagcac
     7441 acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat catcaccact
     7501 ggatatgtca tagtcttgcc atccactgta gcccagcacc cagtcgccag actgataatc
     7561 aggatgattc gactccacga cacggctcac cgtaccgccg accatcacgc cgccaatatc
     7621 aaccggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg ggtccaggga
     7681 caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgacat catcttcttc
     7741 aagacggaaa ttctccggaa ccggtgcgcc atgtggacgc gaggccagaa cccaacgtcg
     7801 attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga atccatcaat
     7861 agtagcaggc tcgcaggatt ggttttgttt gcagccgtca ccccccctct gcaacacgca
     7921 ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga taacatcatc
     7981 atcattattt tattgaggcc agacatgtcc atccgttttg cccgcaaagc cgactgtgct
     8041 gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa
     8101 acggtggatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc aggttatccg
     8161 gtgctggtaa gtgaagaaga tggcgtagtg acgggatatg cctcatttgg cgactggcgt
     8221 agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc cgatcatcag
     8281 ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg agattgcggg
     8341 aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cctcgctgca tctgcaccag
     8401 tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtgg gcactaaatt tgggcgctgg
     8461 ctggatctga catttatgca gttgcaactc gacgaacgca ctgaaccgga cgcgattgga
     8521 tgaatcagtc gctcaccctt gcctttttga ttgccgccgg aattggtctg gtagtccaga
     8581 actcgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc atgttgctga
     8641 actcactggt ggggattgtg ctctttgttt ctattttatg gtttaaacaa ggtatggcgg
     8701 ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacctggc ttgctgggat
     8761 cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg acaactattg
     8821 cagtactggt cgccagccag ctgattggcg gtctggttct ggatatcttc cgcagtcatg
     8881 gcgtaccact acgggcgttg ttcggcccaa tttgtggcgc aattttactt gtggttggcg
     8941 cctggttggt agccagacgc tcattttaat cacaggatgg tgccgccttt ggttaactgt
     9001 tcatggcgcg cttccatatc ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc
     9061 aaacgctgct gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg
     9121 attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg
     9181 atgacttcat ccagatgcga catactttct ccttagccac ttgtgaaatt tatcgattca
     9241 tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt aaaacagtga
     9301 aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa aggcgcttaa
     9361 gaaacggatg cgtttcttat cgtgagaaag aaaccaagtg ataagaaatg cgattgctgc
     9421 cataaccgca taaaaaaaaa gcatgtgttg atagagagac atgtacgggc gtccagccag
     9481 atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt gcatcaagtt ttttaaccgg
     9541 tttgagaaaa aaacatcaaa cagcaacttg cccttatcca tcttcagaat gagtattatt
     9601 gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg gcaaaaatga
     9661 atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt ttgtttactc
     9721 ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc agcacgtact
     9781 gtcagagaaa tcagtgctct gacagtacgc aagatcatct catactctta cgatctgcaa
     9841 tattatcctt aatgtttaac catgacgtgg cggacgacgg tgtaatcctc cagcccataa
     9901 agtgacatat ccttgccgta accagaaagt ttctgcccac cgtgcggcat ttcacttacc
     9961 agcatgaaat gggtattgac ccaggtacaa ccatattgca gccgtgcact gacgcgatgc
    10021 gccctgccca catctttcgt ccataccgaa gatgcaagtc cgtactggct gtcattcgcc
    10081 cagtttacca cctgttcttc gttgtcgaag agcgtaacac tcactactgg accaaatacc
    10141 tctttttgca cgatggcatc gtcctgtaat gcgccagcca gcagcgtcgg cgcatagtaa
    10201 tagccattac ccttgcgctt ttcaccgcca gtgatcactt tgatgtgccc tgtcgctttc
    10261 gcctcttcta ctgccttgct gacgcgttcg agatgcgcca gcgagcttaa aggtccaagc
    10321 tccgtagact cgtcatccgg cgcaccagat tttaacgttg ccactgcagc accgagtttt
    10381 tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc gacaagccac agtacaatcc
    10441 tgtccggcat tgtaatagcc aaatgtacgt acaccttcga ccactgcttc aatatccgca
    10501 tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg cttaatggag
    10561 ggcgcggtat ggctgatgat gtgctcgccg gtggcgatag agcccgtcag cgacaccatc
    10621 cgcactttgg gatgaccggt cagcggatcg cccaccgttt tgcctctgcc aaacagtacg
    10681 ttaatcacgc ctgccgggaa gatatctttc gccagctctg ccaacttcaa cgcggtcagc
    10741 ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc cggagccagt
    10801 ttccacgctg ccatcatcag cggataattc cacggtgcga tagaagccac gacccccaac
    10861 ggatcgcgac ggatcatcga agtatgacct tcaagatatt cacctgccgc cagaccattc
    10921 agacagcgcg ccgcacccgc gaaaaagcga aaaacatcga caatcgccgg gatttcatca
    10981 ttgaacgcac tatgcagcgg tttgccacaa ttacgggact ccagttcggc aaaaacctga
    11041 ccattttctt cgataacatc agccagtttc agcagacatt ccgcacgcac tttcggcgtg
    11101 gtttgccccc attcggcaaa tgctgcatct gccgcgcgca cagcagcatc gacctgctct
    11161 gcggatgcct cggcaatctc cagtaaaacg tcccccgttg ccggattata gacaggctgt
    11221 ttttcccctt cgccgctaac cagttctccg ttaatcagta acttatgttg catagcatat
    11281 tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca ggctcccagg
    11341 atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttagttac cggtacatca
    11401 cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg acctgccgta
    11461 aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc tcctgccagt
    11521 aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc attggcccca
    11581 agatccattg acgcctcaac cagactccat gaggtgcggc gaaaacgggc gatgacattg
    11641 ttaaacacca caactacaca aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc
    11701 tccagattga tggttttaaa ggcggttaat aacgccagac cagtgacaat gcccggcagc
    11761 gcaatgggca gcagcagtaa cagcgaaatg gcgtttttgc cgaaaaagtc tcgtcgccac
    11821 agcgcggcag ctgccagcgt ccctaacacc agcgccatta atgtcgccag cgccgccact
    11881 ttaagtgaca gtgtcacggc atcaagaata tcactacgct gtgctgccac gctaaaccag
    11941 cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat cttcagtgtt aaacgcatag
    12001 gcggcgatta tcaggatggg aaaatgtagg aaaacaacgc cgccccaggc cgccagtttg
    12061 aggaaaaacg gtgcgcgttc agagtgcatc aaacgctccc agacgtttca cgaacgccag
    12121 gtacagtgcg ataagaacaa tcggcaccag ggtgaatgcc gccgccatcg gcatattgcc
    12181 aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggcg ggccaaccag
    12241 ctgcgggacg ataaaatcgc ccagtgtgag tgagaaggta aagatagagc cagcggcaat
    12301 acccgggatc gccagcggca acaccacata gcgaaaggtt tgtcgtggac gtgcgccgag
    12361 atcagctgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct gcacgggcag
    12421 gatcatgaac ggcagccaga tatagagaaa caccagaaag cgccccagcc cggaagttga
    12481 cagcgtattg ccgcccaccg caggtaatgt aaggaacgct gtcagcagtg gttccagccc
    12541 aagatgttgt aaaaaccact gagtcacgcc atcttttgcc agcaataacg tccaggcata
    12601 ggctttaaca atgtagctcg cccacatcgg caacattacc gcaatataaa aaaacgcttt
    12661 cattttcccg ctggtatagc gcgccatata ccacgccatt ggaaaagcca gaatggcgct
    12721 ggcgatagtg actgccacag ccatagtcag cgtgcggaga atgatgtcgt aattcgccgg
    12781 attaaacagc gcacggatgt tcgccagcgt cagttccggc gttaccgaca tggtgaaatc
    12841 gtcaaaggta taaaagccct gccataacag cgttagcagc gagccgaaat agacaatgcc
    12901 aaaccacatt agcgggccaa gcagcagcaa aaacagcccc agccccggat tccgccagaa
    12961 gaaaccggac accttaccca gacctggacg tgaaggtgat tgcaatacgt tcatcgccat
    13021 tcacctctcc tcaaccagcg gcaccatcac atcacgcgac caggaaacca tcacctgttg
    13081 tccgggcgtg agcgtggcag gcagttcttc gcctgtcata ttggcctgac tcaccagcag
    13141 tttttcaccg ccgttcaatt tcagttcaaa acgagttgcc gcgccctgat attgcaccgc
    13201 ctggatcgtg ccattggcct gcagttcacc aggggtgttg aggcggatat gttccggccg
    13261 cagggcgaag cttcccgtca tgccacaaag tttctctgcc atcagtccat caaaaacatt
    13321 cgatgtacca acgaacccgg caacaaacgg cgtgcgcggg cgcatataga gatcgcgcgg
    13381 ggaatcgacc tgctcaatgc gtccattatt gaaaaccgcc acacgatcgg acatcgataa
    13441 cgcttcgccc tgatcgtggg taacgcagat aaaagtgata ccgagagact gttgcagttt
    13501 tttcagctca agctgcatct gctcacgcaa tttgagatcc agtgcgccga gcggttcatc
    13561 caacagcaat acgcgcggtt cattcaccaa tgctctggcg atagcaaccc gctggcgctg
    13621 accaccagaa agttgtgacg gtttacgttg ctgtacaaac cccaacgcca ctttctccag
    13681 cgcctcttgc gccattgcgt gccgctgctt tttattcacg cctttgacca tcagcccata
    13741 ggcgacattg tcaagaatcg acatatgcgg aaatagcgcg tagtcctgaa agacagtatt
    13801 cacgtcccgc tcccacggtg gcagattgct ggcgggttta ccaaagatag agatagcccc
    13861 gccggaaagc tgttcgaagc cagcaatcag gcgcaggcag gtggttttgc cggagccgga
    13921 cggccccagc atagagaaga actcaccatc ttttatcgca atactgacgc catctactgc
    13981 gcgcacgtca ccgtacaacc gcgagacgtt gtcaaactcc actgcgtacg tcataaagca
    14041 ccccagcgaa gttagcgacc gcccataatg gcaatgtaat cctgcgtcca gcgactgtag
    14101 ggaacaaact tgcccccttc tgctataggc gttttccaga aggctatttt atcgaaatag
    14161 ttaaaaccgt ttgtttcaca acctttctcg cctaataacg aactggcttt acacccttcc
    14221 ggcacgactg gtaacgagcc aaaccaggcc gccacatcgc cctgcacttt tggcgttaat
    14281 gaccagttca tccatttgta ggcgcaaacc ggatgtttcg cttcgctatg tagcatggtg
    14341 gtgtcagccc aaccggtaac gccctccttc gggaaaacgg tagcgacagg ctgaccttcg
    14401 gctttcaggg cgttggtctg atagggccag gcactggaag cgaccacacc ctcgtttttg
    14461 aaatcgctca tttgcacggt agtgtcatgc cagtagcgat ggatcaaact atgttgatca
    14521 cgcagcactt tcagcaccgc ctggtactgt tcttcggtga gttgatacgg atcgctgatg
    14581 cccaactgcg gctgagtggc tttaacgaac aacgcagcgt ccgcaatgta gatagggcca
    14641 tcataagcct gaacgcggcc tttattgctc ttgccgtcct gcagattttg ctcaacaaaa
    14701 accacttgcc agctatccgg cggcgtcggg aaggttttag tgttgtacat cagcaggttc
    14761 ggcccccatt ggtaaggtgt gccgtaaact ttgccgccaa cgttaaacca gtcgccttta
    14821 accacgcgcg gatcaagcgt tttccagttg ggaatcaagg cggtattaat cggctgcacg
    14881 cgtttaccca taatcaaacg cagcgaggca tcgccggatg ccgtaaccag atcgtaaccc
    14941 cctttggtca tcagactgac catctcatcg gaagtcgcgg cggttttaac attcaccgcg
    15001 cagcctgtct ctttttcgaa ttgcgttacc cagtcgtatt gtttatcagt ttgtccgcgt
    15061 tcgatatatc ccggccaggc gataatatcc agtcgccctt ccggtttatc taaattggta
    15121 ggcggttcgg cggcgtgagc ggtcattatt gtcatgctga gcgcacacag gctgctgcgg
    15181 gcaaatgtct tgctcataag gtcttactcc tgtcgtaatt aattgttcag ccgccgtgcc
    15241 gtaaaaatat ctccttgttt aaccatagac ggcggcttgc aggcacacct tcgcccctga
    15301 gaaaatttaa tcaggttgtg agctaccgcg cattatgccg tctgtaaggt tatcttctgg
    15361 agtatagaca aggagttagg aaatatgact gctattcgaa attcatatta cccaatctaa
    15421 gaggaattaa attaaacgct attacagccg ttcttgaata agtttgccta attgttttac
    15481 cgcctgttct tcacgttctc cccacagcca cgcggtatta aaacgaaaaa aacgtgacca
    15541 gttttcaccg gtagaaaaca ttttacctgg cgcaatactg atatgatgcg tcagtgccgc
    15601 caggctcaat tcgccggcat ctaacggctc ggggagctcc agccagagaa aataaccgct
    15661 gtcattatga tgaattttca cttccgcagg cagataacgc agcagtgcct gccaggcacg
    15721 ttgtttacgt tccgcaagct ggcgacgcag gcgacgaaga tgggcgtcgt atcggcgcgt
    15781 ggaaaggtaa tccaccagcg caagctgcat cggtgagctg gtggaaagcg tactcatcag
    15841 ctgcaagcgt tgaattttac gtgcatgttt tccggcggcg acccaaccaa tacgaaaacc
    15901 aggcaccaga catttcgaaa acgaagagca atgcaaaacg ccatcgtggc gatcccacgc
    15961 tttcgcaggc agcggttttt cccgtccaaa ataaagttcg ctgtaaacgt catcttcaat
    16021 cagcgttacg ttgtactgat tgagcaacgc caccagttgt gctttttttt gcggcgttaa
    16081 ggtaaatccg agtggattct ggctattagt catcagccag cacgctttca ccggatactc
    16141 ctgcaacgcc agttccagcg cctgaagatc gatcccttct ttaacatccg tcgccaccga
    16201 taacgccttc agccgtagcc gctccagcgc ctgcaacgca ccgtagaaac aaggattctc
    16261 tactatcacc caatcgcccg gttcagttac cgcttgcaaa ctgaggttta atgcctctaa
    16321 cgccccggca gtgatgacaa tttcatcagg agaaatggtg atcccctgta aggcatagcg
    16381 acgggcaata gcctgacgca gttctgcgtt tcctggcggc aagttttcaa tcacgctcat
    16441 cgccgtggcg gttttgctta cctgcgccag cgagcggttt agttgttgga gggggaaaag
    16501 tcgcgggtcg ggaaaggccg aggcaaacgg aacgaccgac ggatcgcgac tggcctgcaa
    16561 catatcaaaa atataagtgt tgatatcgac tgcttcatct cgagtgactg gaatgactgg
    16621 cactttcggc atttttattg cctgtggcgc aacgtaataa cccgactgcg gtcgtgcgat
    16681 aatatatccc tgactttcga gcaactgata ggcatggctg acagtcataa agctcatgcc
    16741 agaaagcgcc acctggtcac gcaacgaagg taaacgatcg ccgggttgcc agatacccga
    16801 cgcaatctgc tcgcgtaatt gttctgcaag ctgctggtat tttttcatta tcgaatcgta
    16861 attatgtgca gatgattcgg cagtctatat cagttgttaa aaatgacaac ttttcgttaa
    16921 ctgtaattaa accatcacca gcgataactc tttgccaagc gcctttgccg cgagctggac
    16981 tgcgtcgatt tttgtcgcat gatgcaagtt aaataggcga gtaatctctt gtttaggttt
    17041 gccaattcgc ctggctaact cttgctgagt gatttctgac tgtaaaaaag catttaacag
    17101 caatacctta gaggcgacgc tcaaaggtac ttcaataaag tgatcatgac tatttaatgg
    17161 cgaaggtaaa gggataagct cgttatcttc aaaataaaaa tcaaatgcgg tcagtaaagc
    17221 atcttttgcc gcttccatcg cttcagcgac agtttcgccc tgcgtcaacg cttcagggat
    17281 atccacaaaa gaaaccatat aaccgccttc cggcgcgggt gtaagagtga cgggataacg
    17341 cataatttcg actgtctctc gcatgtcatt aaccttttta attggcgatt aactcaaacc
    17401 gagttgtttc aggattgctt tacgcaatgg ttctttaatc tcatcgcagg ggtgacgcgg
    17461 catgacactg cgcctcccat gaaacctgag tttcaaatgg ttgctgccat tcgctacatc
    17521 gacgccctga gattcgagcc aacgtctgaa ctcgctttgt ttcacatcgc ctccctgcgg
    17581 ctgagtatat caacaaagta aacattagtg tttacctccg tgtgtaattt gctcgaaaat
    17641 agcactaaat cgccgcaacg gcagaatgca atgcaaaatc aggaaggaag attccagaat
    17701 ttctttgcgc taatttgcaa aactgaataa taacctcaag aaacgatcgg catcatagcg
    17761 gcaaaaggta agaaaaaggt ggcaggatgt ttacgaaggc gttatcggtt gtcttattaa
    17821 cgtgtgcgct gttctcagga caactcatgg cagggcacaa aggacatgaa tttgtgtggg
    17881 taaagaatgt ggatcatcag ctgcgtcatg aagcggacag cgatgaattg cgtgctgtgg
    17941 cggaagagtc ggcggaaggt ttgcgcgagc atttttactg gcaaaaatcg cgcaaaccag
    18001 aagcgggaca acgttgagtc gtttgatgcc ggatgcggcg taacgcctta cccggcaggc
    18061 aatatcatgc agattcaata agttgtgagt aaccagaaaa ctggcgtttt acccttttcg
    18121 cttcggcaat gttttcagca gctcatccgg acttacggat gccactacgc ttcccggtaa
    18181 cggcattttc agtatgtgat ttttgatttt gccaatgacg tgcatctcgc atgggcggca
    18241 atcaaacttt agcgttaata cttcatcgcc gttaaccagt tgcattggcg tcgccttcca
    18301 gcttttgata ttgcctttcg cctgtttcgg gcacagatta aaggcaaagc gcagacaatg
    18361 cttggtgatc atcaccggga cttcgccctt ctcttcatgt gcctcatacg ccgcgtcaat
    18421 cagctgcaca ccatagcgat gataaaattc acgcgctttc tggttgtaaa cgttcgcgag
    18481 gaaactcaga tgtgtttgcg gataaaccgg cgcaggatca gcaaccggtt tacggctgcc
    18541 gcgctggtaa ctggcaagac gcgcagcatc cagcatgtcg gcagcttcac ggcggaactg
    18601 gtttaacaga ctgtttggta caaacagcgc ccccggcaaa ttaatttgca catcgcgggc
    18661 gtaatagagg gtttgcccca gttttgccag accatccttc agattgttca ttgctttttc
    18721 tgcgttattg gcttcgtcga actgcccatc cagcgtatgc gttatgctga ccccctcttc
    18781 actggtgaga gtcagaatca attgttcctg ccaaccgccc agttcaatgt ctaccgccac
    18841 ccgacgttcg ctagaggttt ttgtcagtgc ctgctgccag ttatgatcaa gattacggtt
    18901 tagtgggtga tgcgggcgaa ttttgtgcaa atctgctggc atttcattgg gccagacgcg
    18961 gtactgattt tctccggttt tctcgaccgt attggcacga aaaccgacga cttcacgttt
    19021 aatcaacacg ttcaggccat cgccatttgc cagtggctcg gtaacggcaa catcgagatg
    19081 atctttcgcc actttcacta cttcacctac cggcaggccg ataaatttcg gcgaatcgaa
    19141 cgcgccaata tcgcctttac gggcattcac aaaataatct gtgctaccac ggtggaaagt
    19201 cttttccgtc gatggaacaa agaaatgttc agtacgacct gatgaagcgc gcgccagatc
    19261 gccacgttct tcaataatgg catcaagcat ctggcgataa tgggcggtga tattcttcac
    19321 gtagctcata tctttgtaac gcccttcaat cttgaaggaa cgtacgccag catcaatcag
    19381 cgcgccgagg ttggcagtct gatcgttatc tttcatcgac agcagatgtt tttcatagga
    19441 aaccacccgc ccctgatcgt ctttcaatgt gtatggcaaa cggcacgctt gcgagcaatc
    19501 gccacggttg gcgctacgcc ctgtttgcgc atgagaaatg tagcactgac ccgaataggc
    19561 cacgcacagt gcgccatgaa taaagaattc aatggtcgcg tccgtggcct ggtgaatcgc
    19621 gcggatctga tcaagattca gctctcgcgc cagcacaatc tgcgtgaagc caacatcaga
    19681 gaggaacttc gctttttcta ctgtacgaat gtcgcactgc gtactggcgt gcagttcaat
    19741 cggcggaata tcaagttcca gaatccccat atcctgaaca atcagcgcat cgacaccggt
    19801 ctggtagagg tcagtaatca gccgttgcgc gggttccagc tcatcatcat gcaaaatggt
    19861 gttaagcgtg acgaaaattt ttgcaccata acgatgggca aacggcacca gctcggcaat
    19921 atctttcagg ctattactgg cattatgacg ggcaccaaaa ccagggccgc cgatataaac
    19981 agcatcggca ccgtgcaaaa tagcttcgcg ggcaatggcg gcatcgcgtg ccgggcttaa
    20041 cagttcaagt cgatgagaag atacggtcac attcttatcc agtagctgaa aaatggcggc
    20101 tattttagcc acaagcgcga cgttgcgaaa tagttttccc gcttaactgc gcgggtaatg
    20161 gatgagggaa tgaaaatgta cggtttgctc cccaccatta cgatagatat gcgtgacgtc
    20221 tgcagcaaat cgaacgcctt ccccacaatt aagggtctgc cattcaccat caacgcacag
    20281 atcaagttgt ccatcaatga cgacgacatg ttcgattacc cccttctcat ggggcgtcga
    20341 ttcgcttatt gcaccgggtg ccatctgaat cgagaagtgt tcaaagcaga gttgtggatc
    20401 ataaggaaac agcgaaatga tcaccatcgc ctgctgttgt ggatcataaa ctgaaggcgt
    20461 agcggactgt ggcggagaaa taaatgtcga aaatggcacg ttcaacccag tggcaatttt
    20521 ccataatgtc gcgaccgtcg ggctggactc attacgctca atctgtccta acattgcttt
    20581 agaaacgccc gtcgcttctg ccagtcgcga aagactccag ccgcgctgct gacgtagttg
    20641 tttaagtgtg gtggataaaa agcgagcgag attttccatg cgactcctcc ggcaaaacgg
    20701 aagtttatca cttgtgcgtt ataacggaca aatgctacgg tgcctgtacg ctataacgca
    20761 cgaggtgact atgcgtctgt tttctgttcc tccacccacg ctactggcgg ggtttctggc
    20821 ggtattaatt ggctacgcca gttcagcggc aataatctgg caagcagcga ttgtcgccgg
    20881 agccaccact gcacaaatct ctggctggat gacggcgctg gggctggcaa tgggcgtcag
    20941 tacgctgact ctgacattat ggtatcgcgt acctgttctc accgcatggt caacgcctgg
    21001 cgcggctttg ttggtcaccg gattgcaggg actaacactt aacgaagcca tcggcgtttt
    21061 tattgtcacc aacgcgttaa tagtcctctg cggcataacg ggactctttg ctcgtctgat
    21121 gcgcattatt ccgcactcgc ttgcggcagc aatgcttgcc gggattttat tacgctttgg
    21181 tttacaggcg tttgccagtc tggacggtca atttacgttg tgtggaagta tgttgctggt
    21241 atggctggca accaaggccg ttgcgccgcg ctatgcggta attgccgcga tgattattgg
    21301 gatcgtgatc gttatcgcgc aaggtgacgt tgtcacaact gatgttgtct ttaaacccgt
    21361 tctccccact tatattaccc ctgatttttc gtttgctcac agcctgagcg ttgcactccc
    21421 cctttttctg gtgacgatgg catcgcaaaa cgcaccgggt atcgcagcaa tgaaagcagc
    21481 cgggtattcg gctcctgttt cgccgttgat tgtgtttact ggattgctgg cactggtttt
    21541 ttcccctttc ggcgtttatt ccgtcggtat tgcggcaatc accgcggcta tttgccaaag
    21601 cccggaagcg catccggata aagatcaacg ttggctggca gctgccgttg caggcatttt
    21661 ctatttgctc gcaggtctgt ttggtagtgc cattaccggg atgatggctg ccctgcccgt
    21721 aagttggatc cagatgctgg caggtctggc gctgttgagt accattggcg gcagtttgta
    21781 tcaggcgctg cataatgagc gtgagcgcga cgcggcggtg gtggcatttc tggtaacggc
    21841 aagtggattg acgctggtcg ggattggttc tgcgttttgg ggattaattg ccggaggcgt
    21901 ttgttacgtg gtgttgaatt taatcgctga cagaaaccga taataaaaag cgccatctaa
    21961 cgatggcgcg tctggaacta ctttttgtta acgtcaaaca tggtggcatc ggtcgccatg
    22021 tcatcaataa cttgtttaat attttcgaaa gccattggcg tactttcgtt attcaggtct
    22081 ttaccttcgc cctgacgaac gactttgata accggtttat tagtcgctgc atcaatcagc
    22141 tcaccttcaa aatacaagcg agtatccatg gtacggtggc ctgtagccat ttgcgtcccc
    22201 gcaaccacta atgcaacagg aaccacttca tagaattgca gcccttcttt gctggtatct
    22261 acaccggtaa tggccccacg gaaaatcaga ctacgcggcc ccgcggtggt aaccagtggt
    22321 ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat agttcaaaat tttatccaga
    22381 actttctgcc ctacctgggt cgaaggtttc ggtaccggat aataagtgat tgggttccag
    22441 acgatgctgt catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa aaccggttta
    22501 cctgtagccg aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc agaatattta
    22561 tctggctggg tgattttaga ggcacaacca gacagagcca acaagccgca taaagctgca
    22621 actttcgcaa atgatgtggt acgcatgaat a
//
LOCUS       DAZFKY010000068        22475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFKY010000068 DAZFKY010000000
VERSION     DAZFKY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            325..4137
                     /locus_tag="ACHKCL_002284"
     CDS             325..4137
                     /locus_tag="ACHKCL_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO5763247.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4226..5845
                     /locus_tag="ACHKCL_002285"
     CDS             4226..5845
                     /locus_tag="ACHKCL_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5763248.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            5861..6229
                     /gene="acpS"
                     /locus_tag="ACHKCL_002286"
     CDS             5861..6229
                     /gene="acpS"
                     /locus_tag="ACHKCL_002286"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5763249.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6243..7004
                     /locus_tag="ACHKCL_002287"
     CDS             6243..7004
                     /locus_tag="ACHKCL_002287"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO5763250.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7008..7556
                     /locus_tag="ACHKCL_002288"
     CDS             7008..7556
                     /locus_tag="ACHKCL_002288"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO5763251.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTWRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7578..7859
                     /locus_tag="ACHKCL_002289"
     CDS             7578..7859
                     /locus_tag="ACHKCL_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5763252.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            7895..9055
                     /locus_tag="ACHKCL_002290"
     CDS             7895..9055
                     /locus_tag="ACHKCL_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO5763253.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9129..11687
                     /locus_tag="ACHKCL_002291"
     CDS             9129..11687
                     /locus_tag="ACHKCL_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO5763254.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            11692..12915
                     /locus_tag="ACHKCL_002292"
     CDS             11692..12915
                     /locus_tag="ACHKCL_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO5763255.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            12912..13865
                     /locus_tag="ACHKCL_002293"
     CDS             12912..13865
                     /locus_tag="ACHKCL_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5763256.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            13876..14583
                     /locus_tag="ACHKCL_002294"
     CDS             13876..14583
                     /locus_tag="ACHKCL_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5763257.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14567..15223
                     /locus_tag="ACHKCL_002295"
     CDS             14567..15223
                     /locus_tag="ACHKCL_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763258.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15224..16534
                     /locus_tag="ACHKCL_002296"
     CDS             15224..16534
                     /locus_tag="ACHKCL_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5763259.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16543..17331
                     /locus_tag="ACHKCL_002297"
     CDS             16543..17331
                     /locus_tag="ACHKCL_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO5763260.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17328..18716
                     /locus_tag="ACHKCL_002298"
     CDS             17328..18716
                     /locus_tag="ACHKCL_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763261.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            18730..19671
                     /locus_tag="ACHKCL_002299"
     CDS             18730..19671
                     /locus_tag="ACHKCL_002299"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5763262.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            19668..20654
                     /gene="ycdU"
                     /locus_tag="ACHKCL_002300"
     CDS             19668..20654
                     /gene="ycdU"
                     /locus_tag="ACHKCL_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO5763263.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21021..22223
                     /locus_tag="ACHKCL_002301"
     CDS             21021..22223
                     /locus_tag="ACHKCL_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5763264.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5848 a         4965 c         5885 g         5777 t
ORIGIN      
        1 ttgttttatt cggatgtctg aaatgatgtc gtacagaaaa ctacatatca tttcagacgt
       61 cctctaaatt ggattttttt cacccaagga gcaaggatgg gggactttta aattattgtg
      121 ttttagtaag ttaggttctt cgccgttcga tgaaaaaaca ataactctta gttgtttttt
      181 tgagtcctta tttcagctta tttttcgtga ttttttattg cgccaggtga taggatttgc
      241 cgcaatcatt tttgctgata ttccgtgagt catcaaaagt gatctgcatg tccaaaatga
      301 atagcatcaa taaggaaaac gttagtggcg atgataaatt taagtaagga agcaacggtg
      361 gggaaagcat taacccctat tgctatactt atgatgttgt cttttcctgt agcttctcaa
      421 gcggcgggat tagtcataaa aaatggaacg gtatataacg ccaatggtgt gccagtcgtt
      481 gacatcaaca aacctaacgg tagcggttta tctcataata tctgggataa cctaaacgtt
      541 gataaaaatg gtgtcgtttt caataatagc gctaatgaat ccagtacttc acttgccgga
      601 aatattcagg gaaacagtaa tctgacctcc gggtcggcga aggtgatcct gaatgaggtt
      661 acttccaaaa atccttcaac cattaatggg atgatggaag ttgcagggga taaagcggat
      721 ctgattattg ccaacccgaa tggtattact gtaaacggtg gcggttcaat caatacaggt
      781 aaacttacct taaccaccgg gacgccggat atccaggatg acaagctggc cggttactcc
      841 gtgaacggcg gtaccattac gctcggtaaa ctggataacg ccagcccgac agaaattctg
      901 tcccgtaacg tggtagttaa cggcaaagtg tctgccgatg agctgaacgt tgttgctggc
      961 aataactatg ttaatgccgc aggccaggtg accggtagcg tatccgccac ggggtcccgt
     1021 aacggttaca gcgtagatgt tgccaaactg ggcggaatgt atgcgaacaa aatcagtctg
     1081 gtcagcaccg agaaaggtgt gggggttcgc aacctcggcg ttattgctgg gggtgttaat
     1141 ggtgtcagca tcgattccaa aggtaacctg ttaaacagta acgcccagat tcagtctgca
     1201 agcacgatca acctgacaac aaatggtact ctggataaca ccaccggtac ggtgacatct
     1261 gtaggcacta tctcgcttaa taccaacaag aatactatcg tgaatacccg tgcgggtaac
     1321 atctctacga tgggcgatat ctacgttaac agcggtacga ttgacaatac taacggcaag
     1381 cttgcggctg caggaatgct ggcggttgat accaataacg ccacgctgat taactctggt
     1441 aaagggagtt ctgtcgggat tgaagcgggg ctcgtggcgc tgaaaaccgg aacgctcaac
     1501 aacagcaatg gtcagattcg cggtggctat gtgggtcttg aatccgctgc gctgaataac
     1561 aacaacggtg atatccagac caccggcgat atcgccatta tcagtaacgg taatgtggat
     1621 aacaacaaag gtctgatccg ttcgtccacc gggcatatcg ttattggcgc ggcaggtagc
     1681 gtaaataatg gttcaaccaa aaccgccgat accggcagtt ctgactctct gggcattatt
     1741 gcagataccg gcgtagaaat tggtgcgaac aacatcaata acaacggcgg acagattgcg
     1801 tctaatggca acgtctccct gtcaagttac agcacgatcg acgactatgc gggcaaaatt
     1861 ctgtccaaca gcaaagtgat tatcaaggga agctctctgc gtaacgatac cggggggatc
     1921 agcggtaagc agggtattga agtcgccgtt ggcggcagcc tgaccaataa tattggcgtg
     1981 atcagctctg aagagggtga tatctccctg ttagccaact ccgtggataa ccacggcggc
     2041 ttcatgatgg ggcagaacat cacgatggag tcgatgtctg gcgtcaataa caacacagcg
     2101 ctgatcgtgg ccagcaaaaa actgaagata aatgcgcgcg gcagtatcga aaaccgcgat
     2161 ggcaataact tcggtaatgc ttatggtctg tacttcggca tgcctcagca aacgggtgga
     2221 atggtcggca aggaaggcat cgagctttcc gggcagaaca tctataacaa caacagccgt
     2281 cttatcgctg aggatggtcc tctgactctg caggcgcaga acacgttcga caacacgcgt
     2341 gctctggtca ccagcggggc ggatgcatct attcaggttg gcggaacgta ttacaacaac
     2401 tacgctacca cctggagtgc gggcaacctg gatatcgacg cgaccacgct gcaaaacagc
     2461 agcagcggta cgatgatcga taacaatgcg accgggttca tagcatctga taaaaacctg
     2521 tcactggaag tggtgaatag ccttaccaac tacggctgga tcagcggtaa aggcgatgtt
     2581 gatgtcacgg tgaataacgg caacctgtat aaccgcaata ccattgcggc tgaaaagggg
     2641 ctggatattg ccgcgttgaa cggtattgaa aactggaagg atatttctgc tggcggcgac
     2701 ctgacgatga acaccaatcg ccatgtgacc aacaactcca acagcaatat ggtggggcag
     2761 aatattgtta ttaacgcggt taacgatatc aacaaccgtg gcaacattgt cagtgacgct
     2821 gacctgaacg tgacgaccaa aggcaacctg tataactatc tctatatggt agggtatggg
     2881 gatatcgcat tgtcggcaaa tagcgtggcg aacaataacg cgaccatcga agcgacaggc
     2941 gatctgatta tcgattcgaa gggtaacgtg ggtaacaacc gcggtaatct gcatgcgttg
     3001 aacggcgtgt tgtctgttaa aggcaacaat ctgaacaacg ataacggtga aattcgtggt
     3061 tatggcgatg tcacgctggc actgacgggc aactacgaca gctataaggg ttcgctgacc
     3121 tctgaaacgg gcgacgtgac tctgacggcg aacattgtag acaacgccta tggtttgatt
     3181 gccggtgaga atgtttctgt cgatgctaaa tcgacgattt acaacaacac tgcgctgatc
     3241 gcggcgaata aaaagctggt tattaacgct ggcggcaacc tcgaaaaccg cgacgggaat
     3301 aacttcctgc gtaataacgg cgcgctgttt ggaattaccg acaacgttgg cggcatcgta
     3361 ggtaaagaag gtgtcacgct ttctgctcag aacgtctaca acaataacag cagcatcatc
     3421 gctgaaaatg gtccgcttaa tctgctgtcc aggggaacgc tggataatac ccgcgcgctt
     3481 cttagcagtg gggctgatgc catcatccgt gcggcaggga cgttctacaa caactatgcc
     3541 accacgtaca gcgccggtaa tctcgacgtt tatgcggcgt cgttgaacaa cgccagcgat
     3601 ggtcgcctgg aagacaatac cgccacgggc gtgattgcgt ctgacaaaaa cctggatctg
     3661 agcgttgata acagtgtcac taactatggt tggatcagcg gtaaaggaga tgtgcatttc
     3721 aatgttctga aaggcacgct gtataaccgt aatgccatcg cggcggacaa cgcgctgacc
     3781 attaatgccc tgaacggtgt tgagaacttt aaagacattg tggcgggtac tgcgctgact
     3841 attgatacgc agaagtatgt taccaacaac agcaacagta atatgttggg acaaaccatc
     3901 gcgatcaatg ccgtgaatga cattaataac cgtggaaata ttgtgggtga ttattctctg
     3961 ggtgttaaaa ccaccggtaa tatttataac tacctcaata tgctgagtta tggtgtcgct
     4021 ggcgtatcgg caaataaggt tacgaatagc ggtaaagacg ctgttctcgg tggcttctac
     4081 ggtttagcgt tagaagcaaa cgaaactgat aacaccggta ctattgtcgg catgtaagat
     4141 gtaaccaaag acagcagtgg cttttaatat taatgcgtca tgtcacgcag gggttgctgc
     4201 tgtctttatt tagtttgaga agataatgca attacgattt aattttggtt tgctgctcat
     4261 tctttgttgt tttcaggttg cagcgaaaga cagtcttgat aatttgatca agcagcaaaa
     4321 aaatgcagat gaaagcacgt tgcgcgcgca taaattagaa aaaaaagatg tttactcaaa
     4381 tgcacaacgt aatgcgttta ctctgggtga tctgccgcag gaaaagaatt gctttatcat
     4441 taatcggatt gaactggaag ataactttct gggtggtcag ttgatttctg aagtgcaaaa
     4501 agcgattgcc ggacgttgtg tcggtagtgc tggtgtgcaa aaaattgcag ctgccttgca
     4561 ggattattat attaatgcgg gatatatcac aacgcgaatt agtatccctt cgcaggatat
     4621 ttctaaaggc aagctacgtt tccaggttaa tgccgggaag atagaaaaaa cgattgtcga
     4681 aggtaatgat atcagtgaat ggatgttacc ttttcagcgt aacgatattc tcaatattcg
     4741 tgatattgaa caggggctgg aaaatcttca gcgagtacct gacgttgatg tgaaaattaa
     4801 tattgaaccg ggaacacggg atggttacag catcgttcat attgatacac accgggggaa
     4861 aaactggagc gttcgggcaa gttacaacaa ttggggcgat gaagaaacgg gccgttacca
     4921 gacaacctcg gtgggttatc tgtttaatcc agcgaaaatg ggggatctgt tttatctcgc
     4981 cggtacgcgt agtactaccg gacagtatga aaacgtcagt agttattatt ccattccggt
     5041 ggggtactgg gagtacggct ttttctatag caaaagtaaa tcgcagcagg tgattccgct
     5101 ttcgtacatc tcgctggatt atgttggcat cagcgaatac ttgagcgcaa aagcgacgca
     5161 taccgtttat cgcgataaaa caaaaaagtt cgccggtagt gcagaactca ttcgacgaaa
     5221 gtcgaattac acgcttaatg gtgaggagct gacattacag gcgcgggata tgggaaatat
     5281 caagctgggg ctaaattata agcaacagtt acccggcgcg ttctgggatt caaccctctc
     5341 gtggcaacgc tttttaacct ggttcggtgg tgaaaaaacg ccggatatgc aatatggcga
     5401 cgttagcccg gtaagcaata tctttaattt tgagggtaac tatacccgac aaatagagaa
     5461 tggatattac aataccgctt tttttgccca gtacgcaccg cgtgaactga ctttgcaaga
     5521 taaaattaca gttggcgatc gttggagtat tcgcgggttt gaaaatagcg taggtttgtc
     5581 agggaatgat ggtttttata taaaaaatac gctggcattt cctttgcctg gaatgaaagc
     5641 taattattat gccggtcttg attttgggca agtgtatcag gacgcttctt atggcgatga
     5701 atcactggtg ggctcggcag ttggtattga tggaaacatt aaaagtcttg agtataactt
     5761 ttccgtttct accccattaa agtatccggc gacattagat attgatcggg tgaatgtaaa
     5821 ttttaatttt tcgtaccaaa tgtaaaggat aacgagactc atgagaatag ggacagatat
     5881 tgttgaaatt gccagaataa ccaaagccat tattaatgga gagatggctt tttatgagcg
     5941 tgtttatact tctcatgagc gcttactcat taaccctgca gaacctgatt atgaacgggc
     6001 ctctggattc tgggcggcga aagaagcgtt agttaaagcg attggttgtg gttttcgtga
     6061 tggcgtacgt ttccaggata ttgaaataaa gcatgatgca gccaaagcac cttatttcgt
     6121 tatttccgga agactacaag aaatttttat cgggcagggg ttgcaaaata actccctgag
     6181 tatttcgcat tgccgtaccc acgccattgc gactgtcatc gtttattaac tcaacatatg
     6241 tgatgaatat gtacaaatat aatgatttaa atgaaaaaac cgtactggtg acaggagcca
     6301 gtggtgatat tggactgggt atttgcgaaa aatatctgga gcagaactgc gatgtttatg
     6361 cgctctataa atccaacgac gtgcaattaa cggcgctgaa agcaagccat cctgcaggcg
     6421 ataaactgca tattgtgcag tgcgatcttg cctgccccca gagcgtgagc gcattgtgcg
     6481 aacagataga gcgccaagct ggcaagattg atgtgctggt aaataacgcg ggcatcgtta
     6541 aagacagcct gtttgcctcg atgagttatg aggatttcac ccaggttatc gaaaccaaca
     6601 tgttcagcat tttccgtctg acgaaagacg cgctgatgct gcttcgtgcg gcagaaaatc
     6661 ccgccattat taacgttgcc tctattgcag cgctgatccc aagcgtcggt caggcgaatt
     6721 acagcgcgtc aaaaggggcg atcctaggct tcacccgcac gctggcagca gaaatggcac
     6781 cctggggcgt gcgcgtcaac gcggtcgcac cgggaatgat tgaatcgaaa atggtgaaaa
     6841 aagtatcccg cgcagtggtc cgtgcggtaa ccagtaccat cccgttacgc cgtctgggta
     6901 aatgtgaaga agtggcgaac accatcgtct tcttaagttc ctctgcctcc agctacattg
     6961 tcggccagac catcgttatc gatggcggcc tggtcatgcg gtgatttatg tctaaataca
     7021 cgattccttc gaaaatcttc ctcgaaatgg gcggctggcg tcagccgctg ctgatggtcg
     7081 ataaaattgc agattataaa tacggtgaaa atggctttgt cagtgtcgtg aaacacgtca
     7141 cctataacga accgtatcta ctggggcatt tcccggaaga tccgattatg cccggggtga
     7201 ttatttcgga aatcttcggc caggccagcg agtatctctc tttcctgacc gacatttgcg
     7261 atatctggcg cgaacgtttt gaggaagagc tgaagtcgtt acgtgatatt cacgcacgca
     7321 ttcaccgccc tgaaatgctg gagattatcc gtacctggcg cagccaggta cgcggcgtgc
     7381 tggcggctca gaacctgaaa tttaaagata tcgcctatcc gggtgattct attgatgttg
     7441 tcagcaaatt ggctttttcg gatgccagtg gctttaaaca ctattccgtg accgcgtacg
     7501 tcggtaaaaa gctgattagt cagggcacca ttatcaattt ccgtgaaacc aagtaatcca
     7561 ataagagagt aaatattatg tctaccatga atgacgctat tgaacagcgt aaagaagttc
     7621 tgatgaccat taaaactgag atcatccagc gactgaatat tcaacgcgat gaagcgcaaa
     7681 ttgatgatga tactgcgctg tttggtaatg gttttaaact ggactccgtc gatgcaacgg
     7741 aaattgtcgt tctgctggat aaagtatttg atatcaagat cagcgaaagt gacgacccgt
     7801 cctatatgcg cagtatcaat agtctggcca ccttcgttat tcaaaagaaa aacgattaac
     7861 tacttattaa agaattaatt atcaggacac gtttatgaaa tctcttgctg aatttcactt
     7921 gaaaaataat gcggtaatgg gggtatataa caaccgtacc ctaccttctt cttatcacga
     7981 tgctatgacc gaatataaag ccgttcgtga aaacgcgctg ctggtggact attctcacct
     8041 ctctattgtt agcgtcatgg gcgatgatgc ctgggcgctg atcaaccagc tggtttccgc
     8101 agatgtctcc atcattcgtg atgagcaggc aatctactca ctggtgttga atgaagaggg
     8161 caccattcgc ggagacgtct atgtgctgtg cagcattgac ggttattacc tgctttcaga
     8221 agatatttcg gctgccgaat tgatcgccag catgaatacc attctcgaaa aagcggaaga
     8281 actggatatt caatcgatgc ctgaaattca ggatatgcgg gaaaacaact ggggggcaat
     8341 cctgctcgaa ggcccatact cctgggagat catgtctgaa attcatggct ttgacgtgat
     8401 tggcctgcct tactacgaat acatgaatac cgaggaggat ctgctgctct tccgctgtgg
     8461 taaacacggt gagtatgcgt acatgaccat cggtgagcag gcgaaactgg cggagcagtg
     8521 ggaaaaactg ttaaccgttg gcgagaaata cctgatgcag accggcggcc tggattatca
     8581 aaaaatcgta cgcctcgaaa atccgtgctg ggatgccagc ctctgggaag gtcaggcggt
     8641 gaatccggtt cagttgcaga tgcagtgggc ggtgcagtac gacaaagatg attttattgg
     8701 caaggacgcg gtgacagagc tatcccagga gtataccggt aataaactca tcggcatgat
     8761 cgcacaggaa gaatgcgaag gcattgaggc tggcgatcgc gtgctggtgg aaggtcaaga
     8821 cgtgggctat gtcgtgaagg cactcttttc cccagcgttg cagcgtttca ttgccctgac
     8881 cctgctggaa aaggattacg cctggtctga catcagcggc tacgaaattc aaactgctca
     8941 cggaattatt ccggcgcaat caaaatgtat gccgtttatt tataacctga gcatgttggt
     9001 tagcccaact gagcacagtt atatcgacgc gtcaaaaaat aaaagcgcag cgtaatgaat
     9061 atcgccgcgt gactatagct tacgcggctt aaattaaccc caattggcgg gattgccagg
     9121 tattattcat gaaaacgaac aggaaaagag ttgttattac cgggctgggg attttgtcct
     9181 cactggcgga aaatattcag gatttccggg aagcattatt aaataaaaaa aatggcgtta
     9241 aggatagcga acgctttgca aaatggtttg aaaatgcgcg tgcggcagaa gtgttgcatg
     9301 acattgaata ctccgatctg aacgaagatg ttatcgattc actggataac gcagcgctct
     9361 gggcctacaa agtgggtaaa gatgcgctga gtcaggccgg cctgatggat agccgtgaca
     9421 ccctcaaaga aacaggcatg atcgtcggtg tttcgtccgc gggaacagag gcatttttgc
     9481 cgctgttcga acagcgcatg gaagatttct cgctgcgtaa agcactcttt tccggcgcat
     9541 tctcatcctg ttgttccagc gtttcaacgc tgctgggctt acagggtggg gttgaactgg
     9601 tggcgaccgc gtgtaccgcc agcccgaacg ccgtggggat ggcctttgac tacatccaga
     9661 atggtaaaag caaaaccatg ctggcagtgg gcactgagcc catctatctg ccgacctttg
     9721 ccggattcta cgcactgaat gtcatgcatc cagacacttg tacgccgttc tcgggtcagt
     9781 ccggtatgtc gattggtgaa ggcgctggcg ctatcgtgct ggaagagtat gagcacgccg
     9841 tagcacgcgg ggcgaccatt tatggtgaga tcctctctta cgccacttcc tgcgatgctt
     9901 tccatgaaac cgggccagat ccgcgtgcca gcggagcggt gcaggtcatg aacaaggcaa
     9961 tggcaaatgc gggcgtaacc ccggagcaaa tcgattacgt caacgcgcac ggtacgggta
    10021 ccgaagccaa tgaccgtatt gaaacgctgg caatgaaaaa ggtcttcgct aaccacgata
    10081 aactgctggt gagctccacc aaatcttact ttggccacaa cattggcgca gcggggattg
    10141 tggaactgat tgcctgtctg gcaacgcttc cggataaccg cgtactgccg acgctgaatt
    10201 tcagccatgc acgcccgaat tgcgatctgg actatgtgcc gaatgagttt cgcgatcgca
    10261 aagtgaacat cttcatgaag aataactatg ccttcggcgg caacaactgc tgcatgatcc
    10321 tgagcatgga gcccgcgtcc attccggtca gtacctatga ttcgaagcgt gtggctatct
    10381 cgggcgtcgg tgcagtatcc gcaataggcc acacgctcaa ccaggttctg gaaaacatct
    10441 gggcgcagga ccaccacgtt gaactgggta gcgtaacctt cccggacgat acactggaag
    10501 aagccaaaga gctgctggcc gtgctggatg aatcaaacca gtttgccgag ctgtttgatg
    10561 aagagtacag cttcagcgaa gctacgcttc cggaaacgga aagcaacttt aaaacgttcc
    10621 aggtttccgg gctggagccg cgtaaacacc tgcgtcgttt cgatcagcgt aaagcgaccc
    10681 gtggcggcac gtttgccctg atcgcactca ccgaagcgct ggaacaggcg aagcgtaaaa
    10741 tcaaacgcga cggcgatgag ttgggcatga tcatggggat gtcccgcggc ccgcaggaaa
    10801 ccacctacaa atatctgcaa agtctgaaac ccgatccacg caaagtacgt acatcggaat
    10861 ttcctggctc gctgatgaat gcgatcccaa cgttctgcgg gatctctgaa gggatcaaag
    10921 ggtataccac tacgcttgcg acaggggaaa actcggcgct gggcgcgctg acctacggtt
    10981 atgaaatcgt ccggcagaac ctgcaaccgc aggtattggt tggcggcgcg gatgagtact
    11041 tcccgtcgat gtcgctgtat atggatgcgg tgacacaaaa gatccacatg acggctgagg
    11101 cgagcgacta tcagatttac ggtaaagatc cgcaaggtta tgttccgggc gaaggggctt
    11161 gcatgctgct gctggaagat ccgcagttgg ctgtggcgcg tggcgctgag gttctcgcgg
    11221 aagtggtggg ctacggcaag tcatgcagca atacctactt cgacgcctca ttgttggaag
    11281 aaaaatccgc gtcgatggcg ctggccattg aacgcgccct tacgcatgcg ggcatctctg
    11341 ctggcgatat tgatttggta tgtggtacca gcaacggtag cgatgaccat tctcgtatcg
    11401 aaattgacgc gatttacaac accttcgccg cgcaaaaccc ggcggtgccg gtagtgaact
    11461 ataacgcttg ctttggtttt gtcgcctctg ccgctggttt gcttaacctc gccgtgatta
    11521 ctgactgcat taagaagcag gcagtacctg cgattccaaa tacggtggaa ttctttgatg
    11581 agcgtgtgaa ttttgtacgc cagtcgatga aacttgcgct gaaccatgtc ctgcttgttg
    11641 gcgcaacgga aggcggcaac tactacgcgt tcgtcattaa gggataagac tgtggacaat
    11701 gccgctatta tttcgggtta cagcgtgtgg atgccgtatg ccgaagactg ttcgcagtta
    11761 attgacaatc tgaaacaggg gaaacgcgtt gcgcgtacac cctggtttac ctccaacgag
    11821 gaagccatta aatgcgggtt taagggtaat ccgtcagtgg cgacgttgaa acaggtggac
    11881 gatagcgcat tggacctgct gtctcagttg atcgacgaag ccctggaaca ggcaatgctg
    11941 gataagcatt gcctggccgg gcgcaacgtt cgcgtttatc tgaccggtat tgggccgcgt
    12001 atcgacgggt tggattacaa atctttctat aattacaacg atgtagaaga tatcaactta
    12061 acgcaatcca tcacaaatct gcatgcctca aagatgtcgc aggacacgat ttcaagccat
    12121 cttgcgcgca aatatcgctt gcagtatttg ccgcctaaca tgaactgcac cagtaactcg
    12181 tcactgactg cggtgcatct ggcgacgcag ggcattgagc agggcgggat tgatctcgcg
    12241 attgtgctga actgttcgaa aattaaaacc caggacatct ggttcctgga aacgcaatcg
    12301 atgctggaca gcgaacaagt gcagccgttt ggtgaaaaca gtaaaggtgt ggcgttcgca
    12361 gaaggtttta gcgcgctcct actggaaagc gctcatcacc gccgggcgcg tcaacaaagt
    12421 gagggcgtaa gggtgcaaac gacttacacc caaatcagcg cgggccgcag caacgatgcc
    12481 tcctggctta gcactaacgt gcacaaggtc atgcaggccg cgatgaagca ggctgaaatt
    12541 gcgctggatg atcttgcagc cattcttcca cacggcaacg gttcggcagt gagcgataac
    12601 gcggaggcca aagccatcgc catgttcgca ggggagcgac agatccccgt tctcgcctat
    12661 aaagggcaga tcggctatac cgcaaccgga tctggtgttg tcgatctgat cattggccac
    12721 cattcgttga cgcatcatca actgatcgca cctgttggca acgacgtcat tatcgacagc
    12781 atggcttcgc tggtacttac ggacggcagc gtgacaaacc atagcaaacg ccatttgctg
    12841 aaggttggtg tgggtgtcga tggttcagtt attggcgttg tcatgacaaa tatgcaagcg
    12901 gggcgcgcga aatgacggaa aagagtatct acctgtcttc ctgggcgctt acggaaccgg
    12961 acgacatttt ttatttttat cagccgacct ggctcaacgc ctgggaagca catatgtacg
    13021 aaaggggatt atacggcacc cgtgctaatg gctggggact gaagcgcttt ggcgtggaga
    13081 acgatattta tgcaccagaa cggatccgta atccacaata tatgccttac cttgaggcga
    13141 tggagcaggt gaccgattct gcattacaac aaatgccgac taaagaacgc atgaagttaa
    13201 tgaaatcgcg cacggcattt atttatgccg attcctgggg cgaatccggt tcgtttgaaa
    13261 aaatttccag tgcgctgcat atcgccacta ttgatacgct tcccaagaat ttacttaaga
    13321 agttttcgat aacggaaccg acttgcaaga ttcgcggcga aaaacaggca ttcgttcagg
    13381 cgctgcgtat ggcgcaggat tatctcgcct gggatgtatt cgataacgtg gtgatttgtg
    13441 ctgcctatcg ggcaattccg gtgctggtat tctctgaaga agatgtgggt aaaccgccta
    13501 agcgtttatt tccccgtcgt gctgatgagg tgaatttatc tgttgaacga gtgggttgtt
    13561 ttattctcag ccagcgtgag agcgccatca ggctgcaatg cggggaatat cattccggca
    13621 ctgcgccaca gcatgctacg gatattgatg tcatggcctt cagcgaacgg caacaaatgc
    13681 cgtcccggcc tgctgctggc gttatcgacc tggtggccca ttacggtgaa agcggttgtc
    13741 tgacgcccgc gttgagtatg gattatttaa cccgtcatat tcagcccggt ggaaaaatgc
    13801 gtacggtcat tgccgataaa cagtttggtt atcactactt tgaccttgaa taccttgaag
    13861 gataacgaca gtcgtatgca atctactatt aaatcgttgc ccgctatttc gcttagcaat
    13921 atttataagt gttatggcgc tggggcagga aaggttgatg cgcttaaaag cattaatctt
    13981 gatatcaata aaggcgaatt tctcgcactg tgtgggccgt caggcagtgg taaaagtact
    14041 ctgttgaata ttctctcggg tattgataaa ccgaccagcg gaacggtgat atttttaaat
    14101 aaattactga accaactgcc agaagagcag ttggctgtga ttcgtggaaa acatttgggt
    14161 tttatttttc agttttttaa ccttatgccg gtattaaatg ttttcgataa cgtttatttc
    14221 ccactggtgc ttaatggtca gttcagtaaa aaagaagcca ctgaacggac gctgcattat
    14281 ctcgatagtg ttggattaag cggttttggc gatcgtaaac ctgggcaact atcgggcggt
    14341 caacaacagc gtgtcgccat cgccagggca ctggcccacg aaccgatggt ggtcattgcc
    14401 gatgaaccga cgggaaattt agacctggca accggcgagg ctattttgga cctgttgttg
    14461 acgattaatc agcagaccgg caccacattt gttatttcca cgcactccag cgaattaaaa
    14521 gcgcgggcac ggcgagtggt tgaaattcag gatggagtat tgattcatga ctcagatgca
    14581 taaaggattt ttgctcatcg gcgtcttttt gtacgcggcg tttgtggctc cgaagattta
    14641 cgcggcaaca cccgacgctg ttaaccaggt tctgcaacag gggctcccgg cgcagcctgc
    14701 cgcgctggcg acgctgaccg aggcgttaaa gaaagagtat gaagccaggc acaatgtggt
    14761 ggcgctggtt ttttacgcct atggcttact gcgtcaagcg gacggttatg cggcaacaaa
    14821 cgattttatc catgcctcag aatacgccaa aagcggcttt ttctggctgg atgaagcggt
    14881 ggatttgcag gagaaaaatc aacgcgtacg gtatttacgc gcccgcgtag atgcctattt
    14941 acccgcagac tcagggcgtt gcgtggtgac agtcaaagac accgaacaca tgctggctga
    15001 cccggcgata tggacgacaa cgatccgcga ccatattctg gcgatgcgtt atcgcgcatt
    15061 acgtcactgc aaggacacca cccgcgcgaa tgcgttgttg gcgcaaatta aagggcaaaa
    15121 cgccgcgctt gcgcagtcgc tgacgcagga ttttaatgtt gtaccagaat gggatagtga
    15181 agagttaacc caggtactgc tgccgctgat gaaaggtgag taaatgtccg gggaaatttt
    15241 agctgttaat tgcgttctgc tgctgattct gttttttgtg ttttatcagc ttctgtttta
    15301 ttcacgagat gtcaaatttg catttttgaa tctctttcgc cacaagcgtc gctctttttc
    15361 taccataacc gctattgtat tgggtggcgt ggcaattttt ttatatggcg gatttattga
    15421 ttactctttc tggatcctga aagagcagac gattcgtacc aatattggtc atgtacaaat
    15481 ttacaatcag cactattttg aaacgtcgaa taaaaataag agcctgattg ccgattatgc
    15541 ggcgttaaag aaagctattt tgtcggaccc gacgctggcg gcggatattt ccacgctctc
    15601 cgggcaactg gagtttactg gcgtaatttc acattacgag agcgaaacct ccagctattt
    15661 ctccgcgctg ggggtcgagc cgttacccgc tttaaagctg ggttcgttcg ataagattat
    15721 cgcggggagc gatctttccc gggtaaaagg tgatgaaatc accctgggca gcggtctggc
    15781 gaaaacgttg aatgcgaaat atgacgactg gctggatgtg atggtggtca acacggcggg
    15841 cgggcagggc gcattgtcgc taaaaatgcg tggcattttt gagtcaggca ttaaagatta
    15901 tgacgatgtc gcgatgaaga tcccgctgga caccgcgcag cgcatgatgg gtacggatgg
    15961 cgtcagcaaa gtgcttattc tgcttaaaga ggatgacacc gcggcgttta gcgcaaagct
    16021 gcgtcaattt atcgaacgca atcatttgcc gctggtggtc aaagactggc gtgatgtttc
    16081 actcttttat caacaggttg aagggttgct atcggggatc tatttcttca tcaagctgat
    16141 tgttgcgctg attgtcatct tcatgatcgg caactcgatg gcaatgaaca ttgttgagcg
    16201 cacccgtgaa attaccactc tgcgtgccat aggcctgaag ccgcttcacg tcacgcgctt
    16261 gtttctgaca gaaggcattt ttattggtgt gattggcgca gtcggcagcc tgctggtggg
    16321 gggcgtgtta gcctggatta ttaatcttta tggtattgcg atgccgccat cgccgggaca
    16381 aaccatcggc tataccgcct ttataaaaac cagtaacccg gaattgattt gggtgacggt
    16441 ggtattacct attttgacag caacaggtgc ctctgtgtta ccggctttac gtgcttcccg
    16501 gctgaatatt tctgacgcat ttaaattctc atagggctaa ttatgaatat ggctttttac
    16561 ggcaagtggt ttgcttgcct gtggctcgct acgtcgtgcg ttcaggcggc gagcacagat
    16621 aataaggcgc tggagataat tcgtcgcgct gacgaaattc gttcgccgaa taaaccgttt
    16681 cgttacacgc tgacggtaac ggaatataaa gcgggcgcaa cccagccaga gaataaacag
    16741 gtcctcgata tttccatgcg ctttatgaaa ccgcagggta atgaaaaagc ggatgcacgc
    16801 tcgctggtgc gttttattta tccgccgcga gataaaggaa aaatcatgct ttccgactgg
    16861 tacgatttat ggttttacac gccggaactg cgacgtccta tgcctatttc acggcaacaa
    16921 agattaattg ggcaaatctc taatggcgat gtcattgtca ctaactttga atatgcctat
    16981 gactcgaccc tgatggggga agtcacgtgt gctgaaaagc agtgttacaa gctggcgctg
    17041 gtgcgtaaat cggcggatat cacctggccg aaggttatct attacgttga aaaagacggt
    17101 gataatcgcc cgtggaaggc ggcctattat tcgcaggatg accagttaat aaaagaagtg
    17161 ttgtatcagg atttccagcc ggtgttgggg aaaacgcgac ccatgaaaat aaccgttacc
    17221 gatgttcgtc acggcaataa ctattcagtg atggagtaca gcgatgttcg tctggaatcg
    17281 ctgccggagt ttcatttcac caaagagtat attcagcgag gtgcgaaatg aaacgtacgc
    17341 tgaagatctc ttcgttgctg tgtgttgcgc tgccgttgac ggtacaggcg gattgcctgt
    17401 caggtgacga ggtggcacaa aatagcgatg tgacgcttta cgagcaggtg tcttatgtca
    17461 ataaacaggc ttcggcctgg cagattgcag gcaaaaatcc ctacacccgt cacaacggtt
    17521 atcaggaggc ggggattggc atcaatagcg gttgttcgat aatcgacaat acgctggacc
    17581 ttaagctcaa tctgtatgga ataaacgaat atgcgcttaa acccgcaggg aaattcgaaa
    17641 cggatgatag ccgcacgcgt gcgttaatta accgcctcag tctggtctat agcgcctcgg
    17701 acagcgttca atttgaagca ggcaaatttg ccgcgccgtc aggaatgttc tttttgcgtt
    17761 cgccttctga tttgcaaacg cattattaca ccggattcca gtcgacgcgt ttgcacgatc
    17821 ccaaaatgac atcggcgtat caggcgtcat cgtggggcgc aaaaatgagc gtggatacac
    17881 gcgattatgc gttttcggcg agtgtcatac cgaagttagc caccatcgac aaacgctatg
    17941 tgacgtctgg caactggtcg gctaatcagc agggaaacag cgacgaagcc tatctgctga
    18001 gcttcagcga tcatcgcttt ggtgagcaca cgccgacagt caatgtgcgg ctcggaccat
    18061 cgccgtcact cgcgctgtct gacagttatc actacacccc gcagcttacg ctgaatgtcg
    18121 acgcagccta ccaccgctcc cagcaatggc ggcatctttc ccaccgtgag acggcgcagg
    18181 tcgaggaata tcagtttccg gattcgctct atgaaacgaa agatgaaagc ggcgtagagc
    18241 tggcgctggg tgggcagtac accagtgata atttcagcgt gtttggcgtt gagtattact
    18301 ttcagagcga gggttattcg cgcgctgaac agcgtcagca gcgcgagtta attgacttcc
    18361 tcaataccac aaccggctat gcgccactgg atcaggcatt tgattcttac aaatacctga
    18421 tggcttcaga aatcagcaat accgccaatc aggggatgct gcagggtaag cactatctga
    18481 acgcctgggc cagtctgccg ctggcgggag agtcgacgct gcagccttcg ctggtggtga
    18541 atctcgttga tggcagcacg ttgctgggcc tgcattattc cacgccgctc agcgctataa
    18601 gtaaccagct tgaagcctat gccggtggct atagcgcgct gggaagtcgg tactccgaat
    18661 ttgcgctttt tggcgacacg ttaggccttt atttgggctt taaatactat ctataaggat
    18721 aaagagatga tgaataatgc ttcacaaccg acagtactgc tttttgccgg tcagggtaat
    18781 ccggttattg gtatgggcag cgatctttgg gatctcaacg ccagtacaaa acatatctgg
    18841 gattgtgcca gcgatatttc cggactgaac ctgcgtcgcc tgtgtcagaa agggccgatg
    18901 aatcgcctta ttcaaacgac ggtacaacaa ctggcggtga ccgcaattaa tgtaacgctc
    18961 tataccctgt gccgcgagcg tcttgagaat ttaacggttg tgggctcctg cggacacagc
    19021 gttggtgaat acagtgcgct ctacgccgct ggagctattt ctcttgagac gctatttggc
    19081 atgattcact tcagagccag cctgatgcat gacgaaagcc agagaaataa aggcgcaatg
    19141 ctggcggtta aaggcatcaa ccgcgaacaa ctgcagacga tgattacgga atcaggcatt
    19201 gcgctggata ttagctgtga taacacgccg cgccagcagg ttattggggg aacgcaggct
    19261 gcgttgaatg aatttgccac gctgctgatg gccgctggat acgaaccggt taaactcggt
    19321 gtgagcggtg cctggcacac gcgtttgatg gaagacggcg ttcaggcaat gcgcgattat
    19381 cttgccggac tggatatcgc ttcgccagag catcaggttc tgatgaacgt aacagcaaaa
    19441 agcgaggtcg ctccgtcaat tatcaaagaa aatctctcat tacacttaac tcatacggtg
    19501 aaatggactg aatctctcga tacatttctg aatatgccaa cctcggtagc tttccttgaa
    19561 ataagtaata agccctattt ggggaatatg ttcaacgatt tcgctggcgt ggatcagcaa
    19621 cgagttatgc attgtcgtaa agcatttagt gatgcgaagg tattcaaatg attcctgatt
    19681 atttaacttt tattcgcttt caggataaac gaagtctgat atacatttat gctattggac
    19741 ttattctgat aggcttttat tggaaaaatg caggatttac ttttccatca gaggatattg
    19801 gtgtcgttag tgggattctg gccctggtgc tgtataattt tatttttgat ctcaaggcgt
    19861 actgggctta taaatgcgtc acgaagaata tcgatttttc gtggtttaag aaaaagcaga
    19921 accacaaaat agaattattt cttacgcaac ctctggtggc aggatttctg tcgttaatca
    19981 tgttgagtgc aatgagttgg gggctatacc agcgtctacc ctcgttatat gcgctgttcc
    20041 tgatttcgtt acttgggccg ttggttatct ttctgctgtt tcggatgatc cgcaccagtt
    20101 atgtcaagca ggtcgctatt tcagtagcga aaaaagtaaa atataaaagt ctgactcgct
    20161 atgtgctgct ttcggtgtgc atctcaacgg ttgttaacct gcttactatc agcccgttgc
    20221 gtaacagtga ttcttttgtg atagaggggc agtggttaac gtttaaatcg ataattgcat
    20281 tgctcattct ttgtggcgta gtgttggcga ttaatctgtt ttttctgcgc ttctccaggc
    20341 ggtacgcttt tttgggcagg ctttttttgc aggaaatcga tctctttttc tccagtgaaa
    20401 atgcattgtc gacctttttt gccaagccgc tttggcttcg gttattcata ttgctggtta
    20461 ttgaagtgat gtggattacg ctggtgtcgg tattggcaac gcttgtggaa tggcggattt
    20521 ggtttgaagc ctatttttta ctctgctatg taccgtgctt aatttactat tttttctatt
    20581 gtcgattcct ctggcataac gattttatga tggcatgtga catgtatttc cgttgggggc
    20641 attttaataa gtgaggaagt gataggaagt gaccagataa tacatatatg ttctgtactc
    20701 tcttgcgcat tttgattgtt gactgagcaa ccagacagtt gatgtgcacg atttcccctc
    20761 gcccaaacag acgtgggcgg gggcacccgg tgtgtgcaat atacgaaaaa aaagcccgta
    20821 ctttcgtacg agctcttctt taaatatggc ggtgaggggg accgcctgaa ataaatctga
    20881 cttaatccag gggtgttata taatttcatt ataaacaatg tattacactt tttgttgtcc
    20941 gatattatct aatgtattct tagtgaagcg agagactaat ggcatactgg atggcatacc
    21001 agagtgacgg gaattagcgt atggcagtat tgacggatac gaaagcaaga catatcaaac
    21061 ctgatgacaa accattgccc catgggggaa ttaccggact gacccttcat ccttcttcag
    21121 taaaggggcg ggggaaatgg gtttttcgtt atgtaagtcc ggtgacacaa aaaagacgta
    21181 atgctggatt gggaacttac ccagaggtca gtattgctga agctgcacgt actgcccgga
    21241 taatgcgaga gcaacttgct gcaggtgatg atcctctgga gattaaaaag gctgaatctg
    21301 agaaagtcgc tatcccaaca tttgccgatg cagccaggcg tgtacatgca gaactgtctc
    21361 ctggatggga aaatccaaag catgtaaggc agtggttatc gacgcttgag aattacgcgt
    21421 ttcctcaact gggagcaaaa acgctggatt cgattacggc tgcggacgtg gcagaaacac
    21481 tgcgtccagt ctggttaacc ttgtcagaaa cggcaagccg ggttaaacag cgcattcatg
    21541 ttgttatgca gtggggatgg gcgcacggtt tttgtgtagc aaatcctgtt gatgtggttg
    21601 accatttgct tcctcagcag acaagaggac gtgatgaaca ccaacccgca atgccctgga
    21661 ggcagttacc gctttttgtg gcgaccagtg tgtataccga tgaaccttat aatgttaccc
    21721 gcgcactgtt attaatggtg atacttacag caactcgctc gggcgaagcc aggggaatgc
    21781 gctgggctga aattgatttt cataagcggg tatggactat acctgcagaa agaatgaaag
    21841 ccaggataca gcatcgtgtt cctttatccc ggcaggctat ttacattctg gaaaatatac
    21901 gtggcctgca tgatgaactg gtgttccctt cacccagaaa gcagcagatc ctttccgata
    21961 tggtgttgac aagttttctg cgtaaaaaga aagccgtcag tgacattccg gggcgagttg
    22021 ccacggcaca tggttttcgc tcaacattca gggactggtg tagcgaacag gggtattcgc
    22081 gggatctggc ggaaagggcg ctcgctcata cgctgaaaaa taaggttgag gcggcatatc
    22141 atcgtactga tctactggag cagcgtgtac cgatgatgca ggcatgggcg gattatgtga
    22201 tgtctcaaat tgtgaataaa taatagtgaa aatgtatatt gtacgatcat gatttgatat
    22261 gtgatcgtag cagtagtaat taagaaaccg cttttatgtc aaatgtattg agctgataat
    22321 tatcagatcg tcaggaaagc gtattttgat taacagggga ttacttaaca atcccctttt
    22381 tataaaaaat aatttgcctg tttaactctt cactcctgct gagactgaat tattcctagt
    22441 gcgtcataaa tcagtcttct gcgtagttta ttgtt
//
LOCUS       DAZFKY010000069        22145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFKY010000069 DAZFKY010000000
VERSION     DAZFKY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..1630
                     /gene="dgcJ"
                     /locus_tag="ACHKCL_002302"
     CDS             140..1630
                     /gene="dgcJ"
                     /locus_tag="ACHKCL_002302"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5763265.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1673..2176
                     /gene="yeaK"
                     /locus_tag="ACHKCL_002303"
     CDS             1673..2176
                     /gene="yeaK"
                     /locus_tag="ACHKCL_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5763266.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(2177..2281)
                     /gene="yoaI"
                     /locus_tag="ACHKCL_002304"
     CDS             complement(2177..2281)
                     /gene="yoaI"
                     /locus_tag="ACHKCL_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5763267.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2451..2897
                     /gene="yeaL"
                     /locus_tag="ACHKCL_002305"
     CDS             2451..2897
                     /gene="yeaL"
                     /locus_tag="ACHKCL_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5763268.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(2854..3675)
                     /gene="nimR"
                     /locus_tag="ACHKCL_002306"
     CDS             complement(2854..3675)
                     /gene="nimR"
                     /locus_tag="ACHKCL_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5763269.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            3772..4953
                     /gene="nimT"
                     /locus_tag="ACHKCL_002307"
     CDS             3772..4953
                     /gene="nimT"
                     /locus_tag="ACHKCL_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5763270.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            5008..5355
                     /gene="yeaO"
                     /locus_tag="ACHKCL_002308"
     CDS             5008..5355
                     /gene="yeaO"
                     /locus_tag="ACHKCL_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5763271.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5377..5631)
                     /gene="yoaF"
                     /locus_tag="ACHKCL_002309"
     CDS             complement(5377..5631)
                     /gene="yoaF"
                     /locus_tag="ACHKCL_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5763272.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            5814..6839
                     /gene="dgcP"
                     /locus_tag="ACHKCL_002310"
                     /pseudo
     CDS             5814..6839
                     /gene="dgcP"
                     /locus_tag="ACHKCL_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            6872..6970
                     /gene="yoaK"
                     /locus_tag="ACHKCL_002311"
     CDS             6872..6970
                     /gene="yoaK"
                     /locus_tag="ACHKCL_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5763273.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6973..7047
                     /gene="yoaJ"
                     /locus_tag="ACHKCL_002312"
     CDS             6973..7047
                     /gene="yoaJ"
                     /locus_tag="ACHKCL_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5763274.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(7106..7354)
                     /gene="yeaQ"
                     /locus_tag="ACHKCL_002313"
     CDS             complement(7106..7354)
                     /gene="yeaQ"
                     /locus_tag="ACHKCL_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5763275.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7501..7683)
                     /gene="yoaG"
                     /locus_tag="ACHKCL_002314"
     CDS             complement(7501..7683)
                     /gene="yoaG"
                     /locus_tag="ACHKCL_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5763276.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7687..8046)
                     /gene="yeaR"
                     /locus_tag="ACHKCL_002315"
     CDS             complement(7687..8046)
                     /gene="yeaR"
                     /locus_tag="ACHKCL_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5763277.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8219..8857)
                     /gene="leuE"
                     /locus_tag="ACHKCL_002316"
     CDS             complement(8219..8857)
                     /gene="leuE"
                     /locus_tag="ACHKCL_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5763278.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8984..9907)
                     /gene="dmlR"
                     /locus_tag="ACHKCL_002317"
     CDS             complement(8984..9907)
                     /gene="dmlR"
                     /locus_tag="ACHKCL_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5763279.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            10010..11095
                     /gene="dmlA"
                     /locus_tag="ACHKCL_002318"
     CDS             10010..11095
                     /gene="dmlA"
                     /locus_tag="ACHKCL_002318"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5763280.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11346..12956
                     /gene="yeaV"
                     /locus_tag="ACHKCL_002319"
     CDS             11346..12956
                     /gene="yeaV"
                     /locus_tag="ACHKCL_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5763281.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12988..14112
                     /gene="yeaW"
                     /locus_tag="ACHKCL_002320"
     CDS             12988..14112
                     /gene="yeaW"
                     /locus_tag="ACHKCL_002320"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5763282.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14168..15133
                     /gene="yeaX"
                     /locus_tag="ACHKCL_002321"
     CDS             14168..15133
                     /gene="yeaX"
                     /locus_tag="ACHKCL_002321"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5763283.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHQSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15187..16302)
                     /gene="rnd"
                     /locus_tag="ACHKCL_002322"
     CDS             complement(15187..16302)
                     /gene="rnd"
                     /locus_tag="ACHKCL_002322"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5763284.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16384..18069)
                     /gene="fadD"
                     /locus_tag="ACHKCL_002323"
     CDS             complement(16384..18069)
                     /gene="fadD"
                     /locus_tag="ACHKCL_002323"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5763285.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18274..18855)
                     /gene="yeaY"
                     /locus_tag="ACHKCL_002324"
     CDS             complement(18274..18855)
                     /gene="yeaY"
                     /locus_tag="ACHKCL_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5763286.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(18895..19590)
                     /gene="tsaB"
                     /locus_tag="ACHKCL_002325"
     CDS             complement(18895..19590)
                     /gene="tsaB"
                     /locus_tag="ACHKCL_002325"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5763287.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19648..21558)
                     /gene="yoaA"
                     /locus_tag="ACHKCL_002326"
     CDS             complement(19648..21558)
                     /gene="yoaA"
                     /locus_tag="ACHKCL_002326"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5763288.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21690..22034
                     /gene="yoaB"
                     /locus_tag="ACHKCL_002327"
     CDS             21690..22034
                     /gene="yoaB"
                     /locus_tag="ACHKCL_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5763289.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5396 a         5708 c         5430 g         5611 t
ORIGIN      
        1 catacaattt attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat
       61 aattagcgcc cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac
      121 cgaaggagtg ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc
      181 aagtgtcatt gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc
      241 aatgagtgcc tatctgcgct atatcgtgca gaaagcagac tcctcctttc tttatgataa
      301 gtatcagaat cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga
      361 agtgtcgcca gaacagcggc gcgccatctg cgaggctttt gagtctgcca ataataccca
      421 tggcttaaac ctgactgccc ataaataccc gggcttacgc ggcacattac aaaccgcatc
      481 cactgactgc gacacaattg tggaagctgc agcactatta cccgcttttg atcaggcagt
      541 ggaaggcaac cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt
      601 tcactattat ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga
      661 atactttgcg atgaataact ggtccatcct gcagtcagga agtattggca tcgatcgcaa
      721 agatattgaa aaggtattta ccgggcgtac cgtattgtcg agcatttacc aggatcagcg
      781 tactaaacag aacgtgatga gtttgctgac gccggtgtat gttgcagggc agctaaaagg
      841 gattgtgctg ctggatatta acaaaaacaa tctgcggaat atcttttaca ctcatgaccg
      901 ccctctcctc tggcgttttc tcaatgtcac gctaaccgat acagattcgg ggcgcgacat
      961 tatcatcaac cagagcgaag ataatctgtt ccagtatgtc agttacgtcc atgacttacc
     1021 gggcggcatt cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgtggaa
     1081 aagcgttctg ttctggattt tgacggcgtt aattttgctg aatatggtgc ggatgcactt
     1141 ccgcttatac caaaatgtgt cgcgagaaaa tattagtgat gcgatgactg gactgtataa
     1201 tcgcaaaatt ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc
     1261 ttcggtgatg tttattgcaa ttgacatgga caagttaaag ctaataaatg acaccctcgg
     1321 tcatcaggaa ggggatttag cgattacgtt gttagctcag gcgattaaac aatcgattcg
     1381 taaaagtgat tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc
     1441 gacgccgcaa attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc
     1501 gccgcagaaa gagatcggct tctcttccgg tatttacgcg atgaaagaaa acgatacgtt
     1561 acatgatgcg tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag
     1621 ccgttcatga taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga
     1681 aatggctaaa ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc
     1741 tgatttccgc gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg
     1801 tggcaccgct ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt
     1861 aaatcaacat gttctggcga tcctcgcagc cgatcaacag gccgatctga gccaactcgc
     1921 cagccatata ggcggattac gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac
     1981 aggctgtgtc ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc
     2041 cgaccctcta ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa
     2101 atccgttatt ttgaaaaccg cggattattt gcgcattgcg caaccagaac tggtcaattt
     2161 ccgccgcact gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacaa
     2221 cagcaatggc aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca
     2281 tagcataccc ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt
     2341 tgatgacagt tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg
     2401 cagcaagcgt aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg
     2461 tcactttgct gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg
     2521 tcgccgtttc aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc
     2581 cgtggattga aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg
     2641 cccccatcgc cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga
     2701 aatcgctggt ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga
     2761 cgttaatggg cagccagccg caactggttg ctgggctgct ggtgggcact gttttaggcg
     2821 tagcgctgtt tcgcggcgta ccggttggcc cgcttattgc cacgggtctg gtttcgctga
     2881 ttgtggggaa acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt
     2941 cttaaacata gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg
     3001 tacagtatcg cctttcacta atccctgaag cgccataatc agctgtaact gctgtcgcca
     3061 ctggcgaaaa ctcaaccccg tctcttttac tatcagtcgg gcaagattgc gttcactcat
     3121 cgcaaaaaag ccagcccatt gccccaatgc cccccactcg acgggctctt tcgccatcat
     3181 ctccaccatc gcgcggattt taggatgaga agaaacgggt aggtgcagct gttgttgtgg
     3241 ctgctgaggg agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg
     3301 ctgagtcgtc gtcctgtttg ccagagttaa aattaattcc cggcataagg gcgatatttt
     3361 caatgtgtaa catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag
     3421 ttcagcgtta gctgtcactt gattgctatg ttccaccccg cccggtatcc aaaccgcata
     3481 ttgtggcggc accatccaca aagcattttc caccgtacag gtaattgcac catgtagcgc
     3541 cagtatcagc tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc
     3601 gtgaatacaa aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc cattgagatt
     3661 caacctgtgc atcattttgt ccgaatttag cgataatttg tcattttagc ttgattcaac
     3721 ataacaataa aaacggtaag gtacagcctc gtttgtaaca atgagaagca tatgacctgt
     3781 tcaacttcat taagcggcaa aaacaggatt gtccttatcg ctggcattct gatgattgcc
     3841 actacattac gcgtcacctt caccggcgca gcaccgttac tcgatacgat tcgttccgct
     3901 tactcgctga cgactgcgca aaccggctta ttgaccaccc tgccattatt ggcctttgcg
     3961 ctaatctcac ctctggctgc cccggtggct cgacgttttg gtatggaacg tagcctgttt
     4021 gccgcgttac ttttgatctg tgcaggtatc gcaattcgct ctctcccttc gccttactta
     4081 ttatttggcg gtacagcggt cattggcggt gggattgcat taggtaatgt cttactgcca
     4141 ggattaatta aacgcgattt ccctcattcc gtcgccagac ttaccggcgc atattccctg
     4201 acaatgggag ctgcggcggc tctgggatcg gctatggtag tgccgctggc tttgaacggt
     4261 tttggctggc aaggcgcgtt gctcatgctg atgtgttttc ctctgctggc tcttttttta
     4321 tggctgccac agtggcgaag ccaacaacat gcaaatttga gtacttcacg cgccttacat
     4381 actcggggta tctggcgttc gccgcttgcc tggcaggtaa cattgtttct tgggatcaac
     4441 tcactggtct attacgtgat tattggctgg cttccggcga tcctcatcag tcacggctat
     4501 agcgaagcac aggcgggttc actgcatggt ttgctgcaac tagccacagc agctcccggt
     4561 ttgctgatcc cacttttctt acatcatgtg aaagaccagc gtggtattgc agcgttcgtt
     4621 gccttgatgt gcgcagtggg cgcggttggg ctctgcttca tgccagcgca cgcgatcacc
     4681 tggactctgc ttttcggttt tggttccggc gcaacaatga tactggggtt gacgttcatt
     4741 ggtctgcggg ctagttctgc gcatcaggcg gcggcactct cggggatggc acaatccgtc
     4801 gggtatttgt tggcagcctg tgggccgccg ctgatgggta aaatacacga tgctaacggt
     4861 gactggtccg taccactaat gggtgttgca atactttcac tactgatggc gattttcgga
     4921 ctttgtgccg ggagagacaa agaaattcgc taatatccgg tactatagtg acgtgacaaa
     4981 tcatgcgtga aagggagaac aaacacgatg aatattcagt gcaaacgcgt ttatgatccg
     5041 gcagaacaga gcgatggtta tcgcgtactg gtcgaccgcc tctggccacg cggtatcaaa
     5101 aaaaccgatt tagcccttga tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc
     5161 aaagcctttc acggcgaagt cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa
     5221 ctggcacaac acgagcaaga aggaaagcgg ctggcggaca tcgccaaaaa acagccgctg
     5281 accctgctct actcagcaaa aaacaccacg cagaaccatg cgctggtgct ggccgactgg
     5341 ctacgtagct tgtgatttta gtacagcatc cggcggttat ttttcaccag ccggatggtc
     5401 cagtcgccac aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc aattgttgct
     5461 gaccccttgc gctgtctgca ctggaatgag cgtcccgccc ttctgctggc aatagaccga
     5521 cgccggattt gccataccaa tctgcggcgg tttaggtgct tctggctgag aaggggttga
     5581 acaaccagcc aggaccagca agcaaggcag aacaaaactg ataattttca tttattgatc
     5641 tcacatatgt atccaagctt agagtatcgc ggtatcgttt tgttttgcag cactattttt
     5701 attacattca ctcaaaacat attacgtctt gtttcatctt tgttgatgat gttttatcat
     5761 gcctgcaaag attaaataat cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag
     5821 atcagattat cgcccgcatc tcgcaatccc ttgccaaaga acagtcactg gaaagtctgg
     5881 tccgacagct tctggagatg ctggaaatgg tcactgatat ggaatcaacc tacctgacca
     5941 aagtggatgt cgaagcgcgc ctgcagcata ttatgtttgc ccgtaacagc cagaaaatgc
     6001 acatcccgga gaattttacc gtctcgtggg attactcgtt atgcaaacgc gccattgatg
     6061 aaaactgctt tttcagcgat gaagtccctg accgttgggg cgactgtatt gcggcacgca
     6121 atcttggcat caccacattt ctgagcacgc caattcactt accggatgga tcattctatg
     6181 gcacgctttg cgccgccagc agtgagaagc gccagtggag tgaacgcgcg gaacaggttt
     6241 tacagttatt cgccggactg attgcacaat atattcaaaa agaggcactg gttgaacagc
     6301 tgcgcgaagc caatgctgcg ctgattgcgc aatcgtatac cgactcgtta accgggctac
     6361 cgaatcggcg ggcgattttt gaaaatctga cgacgctgtt ttccctcgcc cggcatctta
     6421 accaaaagat aatgatcgcg tttatcgatc tggataactt caaattaatc aatgatcgtt
     6481 ttggtcataa tagtggcgat ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc
     6541 agcaaaatgg cgaagttatt ggtcgtctcg gcggtgatga gtttttggtt gtttcactaa
     6601 acaacgagaa tgcggatatt tcgtcgctgc gagaacgcat tcagcagcaa atacgcggat
     6661 aatatcactt aggtgatgtt gatttgtatt atcctggtgc cagtcttggc atagtagaag
     6721 tcgatcctga aacgaccgat gcagacagtg ccctacatgc tgccgatatc gcgatgtatc
     6781 aggagaaaaa acacaaacag aaaacacctt ttgtcgcgca ttcagcgcca cattcctgag
     6841 gcgtattcac atccttttga ttggtgataa catgcgaatc ggtattattt ttccggttgt
     6901 aatcttcatt acagcggtcg tatttttagc atggtttttt attggcggct atgctgcccc
     6961 gggagcataa agatgaaaaa aacaacgatc attatgatgg gtgtggcgat tattgtcgta
     7021 ctcggcactg agctgggatg gtggtaacgt cacatctaaa aaatagcaaa ggctgcctgt
     7081 gtgcagcctt tgtgcaattt acgcgttaac ttttaatctt cctgtagata aatagcacga
     7141 caatcgcacc aataacggca accacgaagc tgccaaaatt gaagccatcg actttaccaa
     7201 agccaaacag cgtgctgatc caaccgccga ctacggcacc gactatcccc agcaggatag
     7261 tcataaagaa tccaccacca tctttacctg gcatgatcca cttcgccaga ataccggcaa
     7321 taagcccaaa aataatccat gacagaatgc ccattgtttc ctcacttatc tgttttgcat
     7381 tagcgggtta gtcgctgata aaaagcatag cacaacatcg ggagggcaag atttgtgacg
     7441 agcatcacgg aggttttttt gcgatggcgc agaaattgcg ccatcaacga tcagtgataa
     7501 ttaccaacca caaacatcat gttcgttttc cgtgtcataa gaacgcacgg tattcaccag
     7561 atcttttatc acttcagccg ccacttctgg caccagcaaa gtcatcggcg tctctgtttc
     7621 ataatcgaca gaaacgccat tgctgttatt ggtgacggtc acggtatacg ttgctttgcc
     7681 catgattcat ttcccgttat gaatgacttt ccgttgttgc gcaccttcca tcaggacttc
     7741 aggagccacg aagaagtcaa tgttgaaata agtatcgtca gtcatggctt caatgttgtg
     7801 ccacttttct ggagggaaca ccgcaaactg ccccgcttcg ataaggatca cctgatcagg
     7861 ctctgcactg tgttcatcag cgtagccgag atatttgacc gccccatgca taacggaaag
     7921 gcgtgggtaa acccccgggc gcgttccttt atcaagatga cgttcgaata ttccggcagg
     7981 cgcagtttgt ttattccaga aaggcgttga gcgcgtatga atataattct gtgggatttg
     8041 cagcatcctt ttccctcctt cggtgaatgc gctgaaaacg gtttattcca gccgtttcag
     8101 ggtacgcctg ataatttgct ttttaaatac catttattgg ttacttttta gcaccatatc
     8161 agagaaaaat cagggaggat tatagatggg aagcccgcga caacgcgggc tgaaagcatc
     8221 aggattgcag cgtcgccagt cgggcagcga aacccacgaa catcaaacca atcagtgagt
     8281 tgcccacttt agccagtttc tttttggtac gtatgtactg cgtgacaaaa gcaccagata
     8341 taatcaggaa gctcaaatag cagaaactca ccagttccag cgtcgtcgcc agaataaaga
     8401 atgaaattcc cgtatgtggg gcattaacat cgataaactg tacgaaaaac gacacataga
     8461 acaaaatggc tttcggatta gtcaggctca aaattaacgc gcgtttaaaa atagcaccgt
     8521 attggggctc atcggatttg gcctcgttat ttttaccctt cagggtcgcg taaagaattt
     8581 tactccccag atagagcaaa taaaacgcac caagataacg tacaatgttg aataatatcg
     8641 gggtggtctt aattaatgtc gccattccag cccatgccag aaacatcaat accgcatcgc
     8701 caataaatac accgcaggcc gcaagataac cgcctttcat accgctactg acgctatttt
     8761 tgagtacaaa cagggtattt ggccctggca ccaacacaat aaaaatggcc ccaaccagat
     8821 aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta attatctttc
     8881 gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag aaaggagaaa
     8941 atcaggtttt aacctgatat caacccgata attgaatcat taatcaggca tgctccagtg
     9001 aaaaattcgg gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt
     9061 tcgctgacgt cgccagcctt gaaacataaa cggcccagac gttcgctggc tggtaatatt
     9121 ccggtagcac ttgcactaaa tgaccactgg caatgttttc gctaacatcc caccaggaac
     9181 gcagagcaat cccctgccca tcgagacacc actgatggac aatttctcca tgattagacg
     9241 acagtgggcc ggtgacttta atcgcatggt ggccttcttt attgcgtaat tgccagacac
     9301 caaaaggatg atcgcgctct ttaatgacca ggcacggcaa tgcagaaaga tcggttaaat
     9361 gtttcggcgc gccatgttgc gcaataaatt ccggcgacgc gcagagaatg cggtaattgg
     9421 tcgccagttt gcgggcgatt aaattagggg cgatgtcatc gccaatgcga atatcgagat
     9481 cgacaccttc attgaccaaa tcgaccagcc ggtcttccac atcaaaacgt aattccagtt
     9541 gcggatacgc cttcgccagc gctgataacg ccggagccac cacctgtcgt ccaaaaccaa
     9601 agctgctgat aatacgcagc atcccctgtg gcacctgacg cacgtcagaa agttcgtcca
     9661 tcatctgacc gacatcctgc aaaatccgct gcgcccattc ataaatccgc tctccttctt
     9721 cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc gtttgctcca
     9781 gcaaggcgat gcgcttgctg acgaacgccg gtgaaacgcc cagttcttcc gccacggcgg
     9841 caaaaccagc ccggcgagcc accagcataa agacgcgcaa atcattcagc agcggtaaat
     9901 tattcatgat tcgtgtttta tgtttcacca gttacgggga ttaattcctt ttcagtcaat
     9961 tataggatgg tgatgttgtc aattttgatg gtcaggaagt gagaacccaa tgatgaaaac
    10021 gatgcgtatt gctgcgatcc cgggagacgg gattggcaaa gaagtccttc ctgaagggat
    10081 tcgcgtgtta caggctgccg ctgagcgctg gggcttcgcc ttgagttttg agcaaatgga
    10141 gtgggcgagc tgcgagtatt acagccatca cggtaaaatg atgccggacg actggcatga
    10201 gcaacttagc cgtttcgacg ccatctattt tggtgccgtc ggctggccgg acaccgttcc
    10261 ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg accagtacgt
    10321 caacctgcgc ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg gaaaacagcc
    10381 tggcgacatc gatttttacg tggtcaggga aaacaccgaa ggcgaatatt cctcgctcgg
    10441 cggtagagtg aatgaaggta cagagcatga agtcgtcatt caggaatcgg tatttacgcg
    10501 tcgtggtgtc gatcgcattt tgcgttatgc cttcgaactt gcgcaaagcc gcccacgtaa
    10561 gacgctaact tctgccacta aatcaaacgg tttagccatc agcatgccgt actgggatga
    10621 gcgagtggaa gcaatggccg agaattaccc ggagatccgc tgggacaagc agcatattga
    10681 tattctctgc gcgcgttttg tgatgcagcc ggaacgcttc gatgtggtgg tggcgtccaa
    10741 tttgtttggc gatatccttt ccgatcttgg cccggcctgc accggcacca ttggcattgc
    10801 cccatccgcc aacctgaatc cggaacgcac tttcccctcg ctcttcgagc ctgtccacgg
    10861 ttccgcgccg gatatctacg ggaaaaatat tgctaaccct atcgccacaa tttgggccgg
    10921 ggcaatgatg ctcgattttc tcggcaatgg cgatgagcgt ttccagcaag cgcataacgg
    10981 tattctggca gcgattgaag aagtgattgc tcacgggccg aaaacaccgg atatgaaagg
    11041 cagtgccacc acgccacagg ttgccgacgc gatttgcaaa attattttgc gttaaggtca
    11101 aaccagttta tttgaaccgc gtcactgacg cggttttgtt attcgttctt tgcagttaat
    11161 aacctgcgtc atttcacctt ttattgtttc cgtttcgtgt tttatggctt tccgtattct
    11221 taattgttta atttatgtaa catgcaaata atcaattcgt taaacacgac atacaactca
    11281 ttaccctgca aataattaca actggcaaaa gccagagacg ggcttttatg cccaggagat
    11341 gactcatgat gagcaatgtt aagaaaaaag atgtgccgct gataagcatc agcctggtgg
    11401 ccattctttt catcgcagct gcattaagcc ttttcccaca acaatcggcc gacgcggcca
    11461 acgcaatata cacttttgtt acgcgtacgt taggttccgc agtacaggta ttggttttgc
    11521 tggcaatggg actagtgatt tatttagcca ccagtaaata cggcaatatt cgtcttggcg
    11581 aaggaaaacc ggaatacagc acgctctcct ggctgtttat gtttatttgt gccggtttag
    11641 gttcttctac gctttattgg ggggttgctg aatgggccta ttattatcaa acgcctggat
    11701 taaatatcgc accgcgttca caacaggcac tcgaatttag cgtcccttac tccttcttcc
    11761 actggggcat cagcgcctgg gcaacttata cgctggcctc attaatcatg gcttatcact
    11821 ttcatgtgcg gaaaaacaaa ggtctgagtc tttccggcat tattgccgcg attaccggcg
    11881 ttcgcccgca aggcccatgg ggaaaactgg ttgatttgat gttcctgatc gccactgtcg
    11941 gcgcactgac tatttccctt gttgtcaccg cagcaacctt tactcgtgga ctttccgcac
    12001 tgaccggttt acccgataac ttcaccgtgc aggcatttgt gatcctgctt tccggcggca
    12061 ttttttgcct aagctcatgg attggtatca acaacggttt gcaacgtctg agcaaaatgg
    12121 ttggctgggg cgcgttcctg ctgccattac tggtgctgat tgtcggtcca accgaattta
    12181 ttaccaacag catcatcaat gccatcggcc tgaccacgca aaacttcctg caaatgagct
    12241 tattcaccga tccgcttggc gatggttcat ttacccgcaa ctggaccgtt ttctactggc
    12301 tgtggtggat ctcatacacc cctggcgtag caatgtttgt cacccgcgtt tcccgcggtc
    12361 gtaagattaa agaagttatc tggggactga tcctcggcag caccgtcggt tgctggttct
    12421 tcttcggcgt aatggaaagc tatgccattc atcagtttat caatggcgta atcaacgtcc
    12481 cacaggtgct ggaaactctg ggcggcgaaa cagctgtaca gcaagttctg atgtcgttgc
    12541 cagccggtaa attgttcctc gccgcatacc tgggcgtgat gattattttc cttgcctcgc
    12601 atatggatgc agtggcctac accatggcgg cgaccagtac gcgtaatctc caggaaggtg
    12661 acgatcctga ccgtgggctg cgtcttttct ggtgcgtggt gatcactctg atcccgcttt
    12721 ccatcttgtt taccggtgct tcgctggaaa cgatgaaaac caccgtcgtg ctcacagccc
    12781 ttcccttcct cgtcatttta ctggtgaaag tcggcggatt tattcgctgg ctgaaacagg
    12841 attacgccga cattccggct catcaagttg aacattatct cccgcagaca ccggttgaag
    12901 ccctggaaaa aacaccagtg ctccctgcgg gaaccgtatt caaaggcgac aactgagcgc
    12961 catcatccta acgataaagg tatccctatg agcaatctga gccctgactt tgtactaccc
    13021 gaaaattttt gcgctaaccc gcaagaggcg tggaccattc ctgcccgttt ttataccgat
    13081 cagaacgcct ttgaacacga aaaagagaac gtcttcgcca aaagctggat ttgcgtcgct
    13141 cacagcagcg aactggcgaa tgccaatgat tatgtgacgc gtgagatcat tggcgaaagc
    13201 atcgtgctgg tacgcggtcg tgataaggtt ttgcgcgcgt tctataacgt gtgtccgcac
    13261 cgtggtcatc agttgttgag cggtgaagga aaagcaaaaa atgtgattac ctgcccatat
    13321 cacgcatggg cattcaaact cgatggcaat ctggcccatg cacgtaactg cgaaaacgtc
    13381 gccaatttcg atagcgacaa agcgcaactg gttccggtgc gtctggaaga atatgccgga
    13441 ttcgtcttca tcaacatgga ccccaacgcc accagcgtag aagatcagtt acccggtctg
    13501 ggcgcgaaag tgctggaagc ctgcccggaa gtccacgatc tgaaactggc ggcccgcttt
    13561 accacccgca cgcctgccaa ctggaagaac attgtcgata actatctcga gtgctatcac
    13621 tgtggtccgg cgcatccagg tttctccgac tccgtacagg ttgatcgtta ctggcacacc
    13681 atgcacggta actggacgct gcaatacggt ttcgccaaac cgtccgaaca gtcgtttaaa
    13741 tttgaagagg gtacggatgc ggcattccac ggtttctggc tgtggccgtg cacgatgctg
    13801 aatgtcaccc cgatcaaagg gatgatgacg gtcatttatg aattcccggt ggattctgaa
    13861 actaccctgc aaaactacga tatttacttc accaatgaag agttaaccga cgagcaaaaa
    13921 tcgctgattg agtggtatcg cgatgtgttc cgtccggaag atttacgtct ggttgaaagc
    13981 gtacagaaag ggctgaaatc gcgtggctat cgtggtcagg ggcgcatcat ggccgacagt
    14041 agcggtagcg gcatttccga acatggtatc gcccatttcc ataatctgct ggcgcaggta
    14101 tttaaggact aatgacatcg gcggcggtat tttccgccgc cgggctgatt tttgatggag
    14161 tacagcaatg tcagactatc aaatgtttga agtacaggtg agtcaggtgg aaccgcttac
    14221 cgaacaggtg aaacgcttca cgccggtggc aaccgatggc aaaccgctgc ctgcgttcac
    14281 cggaggaagt cacatcatag tgcagatgag cgatggtgat aaccagtaca gtaatgcgta
    14341 ttcactactg agttcaccgc ataacacctc tagttatcag attgccgttc gactggaaga
    14401 aaactcgcgc ggcggttccc gctttttgca tcagcaggta aaagtgggcg atcggttaac
    14461 gatctcaacg cctaataacc tgtttgcgct aattccctca gccagaaagc atctgtttat
    14521 cgcgggcggt attggtatcc cccctttcct gtcgcacatg gcagagttgc aacacagcga
    14581 cgtcgactgg cagctacatt actgctcgcg aaatccagaa agttgcgcat ttcgtgatga
    14641 gctagtccag catccgcagg ctgagaaagt ccatttgcat caatcatcga ccggaacacg
    14701 actggaatta gcgcgattat tggcggatat cgaacctggc acacacgttt atacctgtgg
    14761 ccccgaggcg ctaattgaag cggtaagaag tgaagctgcg cgtctggaca tcgctgccga
    14821 tacgctgcac tttgagcaat ttgctatcga agacaaaacc ggcgatgcat ttaccctggt
    14881 gcttgcccgt tccggaaaag agtttgtggt gccggaagag atgactattt tgcaggttat
    14941 tgaaaataat aaagccgcga aagtggaatg tttatgtcgt gaaggggtat gcggaacctg
    15001 cgaaacggca atactggaag gtgaagctga ccatcgggat cagtatttta gcgatgaaga
    15061 gcgtgccagc cagcaaagta tgttgatctg ttgttcgcgt gcgaagggta aacgcctggt
    15121 gttggatttg tagtttgccg aagccggatg tggcgctgag cgcgcccagt ccggcttcgg
    15181 aaaattttac tgcggatatt cctgcaataa attgtgtaat gcttccgcca tcagctcacc
    15241 acgccagccg gaaatcagct ccggcaaatt gttctgcggt ttcagtttcc agtgccagtt
    15301 cagcagttgg ttgatttgcc gacgcgatgc cagcaattcg gcgctgatct tgtgcgtttc
    15361 gctcacgtca gtaatcagcg acttaatcgc tttaaacgct ttacgataac ccggcatgtc
    15421 catcaggtta agcatcggct gcggtaaggc ctcttccggc aatgtctgcg ctttttccac
    15481 cagcgccagc agcgttttac cgtgaaagcg gatttcgcta ccggataaac ccaggctgtc
    15541 cagttcgcct aaacttcccg gcatataacg cgctaccgac cacaaatgct cttcacgcac
    15601 gacaaagttc accgccagat cgcgctctcg cgccttgcgc agtcgccagt cggctaacag
    15661 ttgcagacag gccagttggc gtgtgcgtaa ttgccaggca ttggtgatat cacgccaggc
    15721 atcttccggc gcaacgactt cctgacgacg catttgcatc aggcgacatt catccagcgc
    15781 cgctggtagc cagccggagg cctccgtttc taccataagc ttggcggtga tcggtaacag
    15841 ataccagaca tccgccgctg catattcaca ctgacgttcg gtcagcggtc tggccagcca
    15901 gtcggtgcgc gattcactct tgtccagcgt aacgccggaa tactcttcca ccatggaagc
    15961 gaaaccccat gacatcgggc gtccgcagaa ggcagcaagg atttgcgtgt caatcagggg
    16021 ttgtggtaat tcgccaaaga cattgaggaa cacttccaga tcttcactgc ctgcatggag
    16081 aaattttgtg atggacggat cgcgcaggat cgctttcagc ggtgaccagt cggtgatccc
    16141 gagaggatca attagcgcca gatgctcgcc atcgaaaagt tgaatcaacc ccagctgcgg
    16201 gtaataagtg cgcgtacgaa caaattcagt atccagggct atcgccggaa aggcacggac
    16261 ggcttcacac aaagaagcca gcgcatcgtc cgtggtaatc atttggtaat tcaaattgtt
    16321 ttctctttag tgggcgtcaa aaaaaacgcc ggattaaccg gcgtctgacg actgacttaa
    16381 cgctcaggct ttattgtcca ctttgccgcg cgcttcgtca cgtaattctc gtcgcaaaat
    16441 ttttccgacg ttagatttcg gtaactcatc acgaaactcc accagcttcg gtactttgta
    16501 gcccgtgagc tgacggcggc aaaaggtcac cagtgactct tcggtaagcg atggatcttt
    16561 tttcactacg aagattttca ccgcttcacc actggagccg gaaggtacac caacagccgc
    16621 gacttcctgt acgccaggat gctgcatgac gacatcttca atctcgttgg gatagacgtt
    16681 aaaaccggaa accaaaatca tgtctttttt acgatcgaca atgcgcagga atccttcttc
    16741 atccattacc gcgatgtcgc cagtgtgtaa ccagccgttt ttgatgattt catcggtagc
    16801 atccggacgc tgccaatagc ccagcatcac ctgtggacct ctgacgcaaa gttcacctgg
    16861 ctgacctggc gatacttcat tatcgtcgtc atccaccagt ttggcttccg tcgacggcac
    16921 cggcaaacca atgctaccgc tatgataatc aatatcatag gggttcacgc tgaccagcgg
    16981 cgcacattcg gtcaggccat aaccttccag cagatactgt ccggtcagtt tcacccagcg
    17041 ttctgccacc acttgctgaa caggcatccc accgcctgca gaaaggtgca gactggagaa
    17101 atccagttgt tgaaactctt tattgttcag caacgcgttg aacaaggtgt ttacgccggt
    17161 gatagcggta aacggatatt tcgccagctc tttgaccagc cccggaatat cccgcgggtt
    17221 agtgataagc aggttctgcc cacccagttc gataaacagc aggcagttaa tggtcagtgc
    17281 aaaaatgtga tacagcggca gcgccgtcac caccagctct ttgcccggat gcagcagagg
    17341 cccatacgta gcgttaactt gttccaggtt cgccagcata ttgcggtgag tcagcatcgc
    17401 gcctttcgcc acacctgtgg tgccgccagt gtattgcagg aaggctaaat cttctggcac
    17461 cagttcaggt ttgacatact gcatacggta gccgttgtgc agggcgctac gaaatgaaat
    17521 ggcatctggc agatgatatt tcggcaccag acgtttgatg tatttaacaa cgaagttaac
    17581 cagtgtgcct ttcgctgtgg aaagctgatc gcccatacgg gtcaggataa cgtgctgaac
    17641 tgcggtttta tcgaccactt tttccagcgt gtgagcaaag ttggacacaa tgacaatcgc
    17701 cgatgcgccg ctatcgttaa gctgatgctc aagctcacgc ggggtataca acgggttaac
    17761 gtttacgacg atcatcccgg cacgcaaaat gccaaacagc gccaccggat attgcaataa
    17821 attaggcatc atcaacgcaa cgcgatcgcc tttcttcagc cccaaccctt gttgcaaata
    17881 agcggcaaac gcgcgactgc gttcttccag cttgcggaag gtcatcacct cccccatatt
    17941 cacaaacgca ggctgatcgg cgtagcgcgc gaccgactgc tcaaacatat ctaccagaga
    18001 ttgataacgg tcagggttga tctccgtcgg aacgtccgcg ggataacggt taagccaaac
    18061 cttcttcaat tcttcacctc taaaatgcgt gttcgtcgtc atcgcaaccc caaatgatat
    18121 acatgccgtt aacataatat taactcatca taccagcttg ataattaccc aacgaaaagg
    18181 ttgcgaagcg cgtcactatt tatttttatc tttaccgtaa gaatgcagaa acagcggacc
    18241 agccgctgtt tctttttctt taaaaacaag caattattca gttacaactg tttgtactct
    18301 cgcggggccg ggattatacc agccccatcc gccgtggcca tagggccagc cacgaccgcc
    18361 ataaaaccag ggatcaatcg gctgaggcgg cataatcacc tgctgggtta aatgccaacg
    18421 tttgtaacct gttgcctgca tcaccataaa tttatagggc gtgttgccga tttttccgtc
    18481 aaccgcacca gtgattggcc cgactaccgt aaccagttgt ccacggaaat ccaccgggtc
    18541 cagaaaaccg ttcacatcgg cataaatgcg accgcgagaa ggttctccca gcgtcggtct
    18601 ggctccgctg tccagcggta cggtagcaat ttccaggcgg gttttccctt gctggttttg
    18661 taccgcaacc actttgccac caaagcgtgc ctcctgacca acgtacagct gcggcgcact
    18721 catcacccga actaaatctt gttgcggcgt gggactgctg cctttaatgg cgtccggcac
    18781 agtgacacaa ccgctcagca ttagcgcaaa cgtacctgcc agtatgcctt tgataacatt
    18841 cttttgaacc gccatgatgc gactcctttt tttctcaggg catactctta agattcattc
    18901 tttgcccgga agtttcttcc atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc
    18961 caccgccacc gttttaccct cggcaaacat ctgacaagca atcggcagca tatcttctgc
    19021 agcgggcagt aaaacttctc cgtcgcgcaa aaccagtccg ctctctttac cgagatccgg
    19081 ccaggcttgc cagcctgtac ctaccgtcac ccattccccg gaaagctgtt gcattcgttc
    19141 atgaacgagt tcgggtttga gtacggcttc ggtttcttca ccgtgccaga taccgttttc
    19201 atcacgctga tattcggccc agtaaacttc gcccattcgc gcgtcaatgg ctgacagcac
    19261 gcgtgttgcg ccgtttttgc gccacgcccc ttgcgccatc gtcattagcg tggagacgcc
    19321 aatcatcggt aattccgcgc caagcgccag cccttgcgcg atgccaatac caatgcgcac
    19381 gcctgtaaag ctaccggggc cgcgcccgta agccagagcg ttaatatcag tcagggaagt
    19441 tccgctggtg gtcaggatat cctgcaccat cggtaagatt cgttgagtat gttcacgagg
    19501 gcaaagctca aaatgagcgt tgacagtacc gtcgttccac agggcgacag agcaggcctc
    19561 tgtcgcggta tcgatagcca gaattcgcat gggtcttcgt gcttagatca ataaaaaggc
    19621 gcgcatcata ccatactccg taacaaatta cctggaggat ggtatcgcaa ggaaacgaac
    19681 cgcacgggca atgtcacggg tgcgtggcgc gggcggcaga ctggcgagaa acgtcgcgcc
    19741 gtaaggacgc atcaccagcc gattgtcaca aattaccaaa accccgcgat cgtcggcgtc
    19801 gcgaatcagt cgcccgactc cctgcttgag agtaatcacc gcatccggca gttgtacttc
    19861 atcgaacggg tcgccaccgc gcaaacggca atcttccatg cgcgctttta acaatggatc
    19921 atcaggcgag gtaaacggca atttgtcgat aatcaccaat gacaatgtat cgccacgcac
    19981 gtccaccccc tcccagaaac tactggttgc cacaagaagc gcgttaccgg cactgacaaa
    20041 ttgctgcaac agttgccctt tgctggtttc cccctgcaat aatacgggaa gcgtcatggt
    20101 agcgcggaac tgctcggcca gatcgcgcat catggcgtgc gaggtacaaa gcataaaaca
    20161 gcgaccgttg ttagcttcga tgatcgggcg cagcatagcc gccagttgcc gcgcggaacc
    20221 cggttgattg gtttgcggca ggttgcgcgg cacacagagt aacgcctggc ggctgtaatc
    20281 aaacgggctt ggcaatagca acgactccgc ctgttctatc ccaagccgcg aggtgaaatg
    20341 atgcagatcg tcgtttaccg acagcgttgc tgaggtgaag atccagctac cgggcttttg
    20401 cgccattaac tctttgaatt tatccgccac gctgagcggc gtgagagcca gagtaaaatg
    20461 gcgcgaagtg cattcgtacc agtagctgta gcccggctga ttgatctctt ttagccgctt
    20521 cagccgtgtg cgatacaacg tggcgcgctc aaatgccgca tccagcaagg cggaacgccc
    20581 cagtgacagt ttcgccacgt cataacaaag ttccagggtg tcatcgagca gtaaaaatgc
    20641 ccgctgaatt tgcggattag ctaacagctc acgcaggtta ccgcgataac ctggttcacc
    20701 gagttgcaga cgaaaatcct gcgcactctg ggcaagacga tcagcgcact tttgtaactg
    20761 ctgggtgtct tttaattcgg tgcggtaggc gatggtgatg tcttttgcca ggtcgagcag
    20821 ttgtcgactg gagagtgact gaccaaaata ctggctggca atgtccggta gctggtgggc
    20881 ttcgtcgaag atcatgacgt ccgcttccgg gatcaactcg ccaaatccac tctctttaac
    20941 caccatatcc gccagaaaga gatgatggtt taccaccacc acatcggcgt ccatcgcttt
    21001 tttacgtgct ttgaccacaa agcaatcttt atacatcggg cagtcgctgc caagacagtt
    21061 gtcgttggtg ctggtgacca gcggccacgc ctgcgaatct tccgccacgc tgacgcaggt
    21121 gctgatatca ccatcgactg tttgattaga ccaggagcgc agcaggatca catcgcttaa
    21181 tatttgtacc ggaagatcgc cccccgccag cgcctgctgt tcgagacgtt cgaggcagag
    21241 gtagtttgag cgccctttca gcaacgccac gttgcccgtg tatttcaacg cctttgagac
    21301 cgtcggcaaa tcgcggctat agagctgatc ctgcaacgct tttgagccgg tcgagataat
    21361 gactttcttt ttcgcccgca gcgcaggggc caggtaagcg taggttttgc ccgtaccggt
    21421 tcctgcttcc accaccagcg gctggccttt ttctatcgcc tgggtgacgg ctaccgccat
    21481 ctgtcgctgt ggttctcgcg gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg
    21541 tgcaaaatcg tccgtcacac taccccctgt tgatttgaac agggattatg tcaggatgag
    21601 ggcgctttcg ccagttgaag tggtgacggc gacctcacat tgtggcagtc tttgcagcac
    21661 gaaaatggaa ggataatgag gataaaatga tgactatcgt tcgtatcgat gctgaagccc
    21721 gctggtctga tgtagtaatc cacaacaaca cgctctacta cactggtgta ccggaaaacc
    21781 tcgacgccga tgcctttgag caaaccgcca acaccctggc gcagattgac gccgtgctgg
    21841 aaaaacaggg cagcaataaa tcgagcattc tggatgccac cattttcctg gccgataaaa
    21901 acgacttcgc ggcgatgaat aaagcgtggg atgcctgggt tgtcgcgggt catgcgccgg
    21961 tgcgctgcac ggtacaagcg ggtttgatga acccgaagta taaagttgaa attaagattg
    22021 tggctgcggt gtaagcttta tcgaagcaaa ataagtcaga cgataattta tcgataatac
    22081 tggtcggtta tacataaatc gaccagagaa taagatatta ctgttgatgc ttctgctaat
    22141 tcatt
//
LOCUS       DAZFKY010000070        22105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFKY010000070 DAZFKY010000000
VERSION     DAZFKY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(160..591)
                     /locus_tag="ACHKCL_002328"
     CDS             complement(160..591)
                     /locus_tag="ACHKCL_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO5763290.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(659..2680)
                     /locus_tag="ACHKCL_002329"
                     /pseudo
     CDS             complement(659..2680)
                     /locus_tag="ACHKCL_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2814..2939)
                     /locus_tag="ACHKCL_002330"
     CDS             complement(2814..2939)
                     /locus_tag="ACHKCL_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO5763291.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(2881..3030)
                     /locus_tag="ACHKCL_002331"
                     /pseudo
     CDS             complement(2881..3030)
                     /locus_tag="ACHKCL_002331"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3204..3965)
                     /locus_tag="ACHKCL_002332"
     CDS             complement(3204..3965)
                     /locus_tag="ACHKCL_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5763292.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(3962..4396)
                     /gene="psiB"
                     /locus_tag="ACHKCL_002333"
     CDS             complement(3962..4396)
                     /gene="psiB"
                     /locus_tag="ACHKCL_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5763293.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4451..6409)
                     /locus_tag="ACHKCL_002334"
     CDS             complement(4451..6409)
                     /locus_tag="ACHKCL_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5763294.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(6475..6708)
                     /locus_tag="ACHKCL_002335"
     CDS             complement(6475..6708)
                     /locus_tag="ACHKCL_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5763295.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(6765..7217)
                     /locus_tag="ACHKCL_002336"
     CDS             complement(6765..7217)
                     /locus_tag="ACHKCL_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5763296.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7243..7449)
                     /locus_tag="ACHKCL_002337"
     CDS             complement(7243..7449)
                     /locus_tag="ACHKCL_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5763297.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            7519..7974
                     /locus_tag="ACHKCL_002338"
     CDS             7519..7974
                     /locus_tag="ACHKCL_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763298.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(8060..8623)
                     /locus_tag="ACHKCL_002339"
     CDS             complement(8060..8623)
                     /locus_tag="ACHKCL_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5763299.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(8670..10031)
                     /locus_tag="ACHKCL_002340"
     CDS             complement(8670..10031)
                     /locus_tag="ACHKCL_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO5763300.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10083..10313)
                     /locus_tag="ACHKCL_002341"
     CDS             complement(10083..10313)
                     /locus_tag="ACHKCL_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763301.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            10830..11339
                     /locus_tag="ACHKCL_002342"
     CDS             10830..11339
                     /locus_tag="ACHKCL_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763302.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(11309..11500)
                     /locus_tag="ACHKCL_002343"
     CDS             complement(11309..11500)
                     /locus_tag="ACHKCL_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763303.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(11497..11919)
                     /locus_tag="ACHKCL_002344"
     CDS             complement(11497..11919)
                     /locus_tag="ACHKCL_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5763304.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(11966..12391)
                     /locus_tag="ACHKCL_002345"
     CDS             complement(11966..12391)
                     /locus_tag="ACHKCL_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5763305.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(12357..12601)
                     /locus_tag="ACHKCL_002346"
                     /pseudo
     CDS             complement(12357..12601)
                     /locus_tag="ACHKCL_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315569.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12576..13352)
                     /locus_tag="ACHKCL_002347"
     CDS             complement(12576..13352)
                     /locus_tag="ACHKCL_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763306.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESAHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(13398..13952)
                     /locus_tag="ACHKCL_002348"
     CDS             complement(13398..13952)
                     /locus_tag="ACHKCL_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5763307.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(13846..14067)
                     /locus_tag="ACHKCL_002349"
     CDS             complement(13846..14067)
                     /locus_tag="ACHKCL_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763308.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(14068..14751)
                     /locus_tag="ACHKCL_002350"
     CDS             complement(14068..14751)
                     /locus_tag="ACHKCL_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5763309.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(14828..15133)
                     /locus_tag="ACHKCL_002351"
     CDS             complement(14828..15133)
                     /locus_tag="ACHKCL_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763310.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(15137..16039)
                     /locus_tag="ACHKCL_002352"
     CDS             complement(15137..16039)
                     /locus_tag="ACHKCL_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5763311.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(16116..17840)
                     /gene="ltrA"
                     /locus_tag="ACHKCL_002353"
     CDS             complement(16116..17840)
                     /gene="ltrA"
                     /locus_tag="ACHKCL_002353"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO5763312.1"
                     /translation="MTEQATTCKGASLLNGDSWHSINWRQCYREVRRLQARIVKATRE
                     GKHGKVKSLQWILTHSFSGRAVAVRRVTENSGKRTPGVDGQTWSSPEVKFLAINLLKR
                     RGYKPQPLKRVYIPKSNGKSRPLGIPTMKDRAMQALYLLALEPVAEVTADQRSFGFRT
                     GRSTADAIAQCFCVLAQKTSAEWVLEGDIRGCFDNISHQWLIDNTSTDRQILTKWLKA
                     GYREKGQLFPVNSGTPQGGIISPVLANIALDGLEALLASEFKKRTVKGRLVNPKVNYV
                     RYADDFIITGESKELLESQVLPVVRRFMAERGLMLSPEKTKITHIEEGFDFLGQNIRK
                     YGGKMLIKPSKANVSSFLKKIRAVIKGNKAMDQLTLIRMLNPMIKGWAAYHQHIVAKV
                     AFNKVDNEIWLALWRWAVRRHPNKGKKWIRKRYFHQQGARNWSFSTATGELLANGKPQ
                     YANLRKAIDTPINRFKPIKIAANPFDPQWEMYFEERCADKMRHKLQGRKKLIQIWFEQ
                     RGRCPICDERITSDSQWQVHHIIRRVDGGSNCLSNLIMLHPMCHTLVHAKGIHVVKPA
                     HESGLRKA"
     gene            complement(19140..20111)
                     /locus_tag="ACHKCL_002354"
     CDS             complement(19140..20111)
                     /locus_tag="ACHKCL_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO5763313.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(20111..21277)
                     /gene="sopA"
                     /locus_tag="ACHKCL_002355"
     CDS             complement(20111..21277)
                     /gene="sopA"
                     /locus_tag="ACHKCL_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO5763314.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(21865..>22105)
                     /locus_tag="ACHKCL_002356"
     CDS             complement(21865..>22105)
                     /locus_tag="ACHKCL_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763315.1"
                     /translation="RKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEI
                     NSRTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         5055 a         6467 c         5458 g         5125 t
ORIGIN      
        1 gcctgagttt aacggacagg ccgatgaagg acgccgttac cgggataatg taagaatctt
       61 tttttgagta tgtcaacata acctgaggca aagtacctca ggttccagta atcagccgac
      121 ctgacagtct tctgttagcc ctacagatat gtcgtgtagc taaccgcgca tgtcaaagcc
      181 ggatttttga aaatctctat aaacttccgg attgttgaag gccgggaatt tagctttatg
      241 ggctgctgac tttatcgctt cgcaataggc tttatcgcca ttactggttg atatttttaa
      301 cgccgtgcca tcccgggaga attccatatg caacctgcat tttttccttt tccatttttg
      361 cggatcatca agtttagcat taattgcatc cctgattccc cgtgcctgct ccccccattc
      421 atcctgatca tcccaacgtc ctgaactgca actacccagg gctgtagttt tatggcaatt
      481 tgaaggatgt aatggcgcac atcccgccac aaagctgact aaaatagcca gtataacgat
      541 tttctttacc cccatatatt gctcctcaac ccatatagat ctcagtacca tagctctgtc
      601 agagcaaaga aaatcttaac taatacaagt taagattttt aatatccact cctcatccct
      661 acattccagg tgatggtggc gtccactcat tatcctgaac tggagcataa gatgcgccat
      721 gccccccgga tatgcttttc tgttgcctga tatattcgtg gtagtactgt tcactacgct
      781 gacgatgtaa atccacatcc gggcggctga ttacaaagtg tccatttccg gttattccag
      841 ctgcattatg ccaggccacc atttccgcaa atacatcatt aaactgttga tatgagcctt
      901 ccggttcaaa acccattcct ggctcacgat caaacaatac aggagcaatc agattcagct
      961 tatccaccag ttcatttctg tcctgatgat ggtagaacac cacacggtcc ccataatcca
     1021 cacagactaa ctcgccattc ctccgatcac gaaaacgcac actgccatct ttctgcagac
     1081 gggcttcgag aacgccagtg tcttcataaa gtggagttcc tcccggttca cagagaatca
     1141 cgcagcggtc ggcattcgtt gttcgtcgct tgtctttgcg acgatcacga taatcccagc
     1201 cacgtaactg tgataatgca gctctgtctc cctgtaccgc ctgttgctcc acccactgcc
     1261 ggtaggacaa aggataccac ttaccctcct caatcagtga caaccgctct tccttcacac
     1321 tctccttcag cctgatcagc gcctgcatac gctgcacttc cgcaatatgg taatgcagct
     1381 tacgcaattg tggatcccgg aactgcacac ggatatacgc cttgcgccga cggcaggcag
     1441 catgaatttc ccgtaaccgt tctccgtaac gtaagtcggg tttacgccag tgctccttcc
     1501 atgcaagata acgcgcgcgc aaatcttccc gcgcagctgc acgggcttca cggcgttcac
     1561 gacgcagtac aggatcgcgc cggaagccgg gctccacttc atcgctggca gcaagttccg
     1621 gattgtagcg acattcaggc ttcacccgtt caaatatatc tgcacctgca atctcaaatg
     1681 gccctgcctg cggctcagcc ctggataacg tcaaatccgg gtggatactg ctggccttca
     1741 ctggtgtgag ctcatgatta aacgcatcca caatcacaag accatgatgc tgtttctgca
     1801 acataagccc tttacgcgca aacatctcat ggcacgcctg ccagctgaaa ttcccgcccg
     1861 cgatcatatc ttcaatcaac gactcacact gaacagttgt gtacccccac agactttcct
     1921 tatcagaaaa cagttcgcgc tttattgctc cctgtggacc tgtccccatc cgcatacgat
     1981 accattcgta atcactgaca gcctgagtac caagattacg tccccggaac cgttctccgg
     2041 gtataacctg agtaaaaata tccttcggta caggctgcca tccaccatcc agatcctgca
     2101 tataatccag cgtcattaac ggccacacac tgtctgcacg tactggtgtc tgttgtttat
     2161 caaatccatc atgcagtacc agatgcccgt gctggatccc catccataat ccagccgttg
     2221 ccagagttct gtgcacatcc aggcaacttt ccagctcacc atttctcgcc atttccggta
     2281 agcgttcggc aaaatgacgg caggcgtact gtttcagact ctccgtttct gccacttttt
     2341 ccggccggat atcaacgttt atggcttccg gatcataacg accgggtgtc cccacctgac
     2401 tgaaaatatc tgtcggtaca ggctgatatt cccccagttt ccgccccagt ttttcagctg
     2461 tccataacgg tccgaacgaa ctgagtgcaa caccttcccg cgctctgtcc cagccatcct
     2521 tcaccaccag ttccccttcc tgcatcgtga tatacagacc atcactggcc agccgtttat
     2581 gcaaagataa ccagtcctgt gcgggctctt cacgcagccc ggcaatcgac atctgagcaa
     2641 tatattccct gaacgtctgc tttttcccgc gtcgccaggc tgttcatgcc cgtacaatgc
     2701 cagcaaggag aaaggggcca ccggcgaacc agcagcccct ttataaaggc gcttcagtgc
     2761 agtcagacca gcatcagccc tgaaaaggcg ggcctgcgcc cgcctccagg ttgctactta
     2821 ccggattcgt aagccatgaa agccgccacc tccctgtatc cgtctctgta acgaatctcg
     2881 cacagcgatt ttcgtgtcag ataagtgaat atcaacagtg tgagacacac tatcaacaca
     2941 caccagacaa gagagctgcg tggtagtttc atggccttct tctccttgcg caaagcgcgg
     3001 taagaggcta tcctgatgtt gtctaagcat gcaggggcct cgtgggttaa tgaaaaatta
     3061 actacggggc ttttgtcctt ctgccacaca acacggtaac aaaccacctt cacgtcatga
     3121 ggcagaaagc ctcaagcgcc gggcacatca tagcccatat accagcacgc tgaccacatt
     3181 cacttttcct gaaaataacc cgctcattca ggctgtttac gggcaatccg tgtgattgtt
     3241 gccgcatcac gcacctcccg gagtttgttc ggcaccatcc accgctccat ctcacggaca
     3301 gcatgaccgg cctcctttgc tgccagactg gcttccgcga taacctgcca gaatggcata
     3361 tcctgccact gccagcgttc cattccggcc agtgacggaa aacgctcgcc ccactgccag
     3421 aacagggttt ccaggtcatg ctctgccacc cgatcactcc agcgtgcata ccagcttccg
     3481 actgtttccg gtgaatggaa cgccagttcg atttcagcct gggcgaccag cgcctggtac
     3541 gcacgggctt tctgctctgc ctccctcgcc tgctggcggc tgtacttctg catgaccagc
     3601 gcggatttat ggcgactgcg ttccctgaca caaaaccgca ccgacgggaa caagcgttct
     3661 gctgcatcac cgggaagggg aaggaggcag acctcgccct gtgtttcaat cagcacatcg
     3721 atggcataca accactgaag acggtttccg tgcagttctt ctgcggtcag ggacggcatg
     3781 aaaaatcgca ggtcagacag cgatatcctg cggcttccgt tcagacagcg gaaaaacgcc
     3841 cccgcatacg gatattccgc cagcgtgttt ccctgattgc ggcgtttttc cgtctctgcc
     3901 actgcccgcc atgcggcctg ttgctctgtg ctgaggggaa ccagtgcctg tgaacgtgcg
     3961 ctcatacctg acctccttcc gccatcagga tatggatgat gtcgttaacg ctgtatccct
     4021 gcgtatccag tgacgccgcc agtgccagtg aatggctgac ggcttccggt gcaaaccgcc
     4081 gctgtacctg aaccaccgca aacggctcac caccggcatt caccagaacc agcacccaga
     4141 cctgggagac atcgcccggt gaacataacg ccaggtgaaa acgttcgtgg gcaggcgtga
     4201 aacggacctg gacggggaaa aagccgccaa actcgctgcc gtactcgccg ttcatcgtcc
     4261 agttgtccgg cgcatccagc tcccgcatca cggcgtgtgt cagctcacgg cgtgcatcac
     4321 ttccggcagc ccggatatct tcatattcct gtgcgttcat ggtatgtaat acattcagag
     4381 tcagttcagt tttcatcacg gtatctccct tgctgccgcc ccgtctccgg cggggcggtg
     4441 tggttgttca tcaggcggca tcagccaggt tatcggtgcg ttcagtggca tccgtctgtg
     4501 ggcgtggtgc acacatccag ccaggcaccc agcggttgtc tttcatatgg tgctctgcat
     4561 gttcagccgc atcgcctttc ttcatcttct ccgcgtcccg tgcggcaccg gatagtcctg
     4621 cctcattcag ggctgcgata atctgcggct ttttcaggtg tccgaaaaag tttgctttcg
     4681 tcggctgcca ccagtcgcgc atgtgaaagc cgatggcgct ctccagcgtg tccagcggac
     4741 tgcgtgacgt gtgaccatac tcacgggtct ggacaccgtg gatactgcac gcggtgcaga
     4801 aactgagcag tgataacagc acctcctgac tgagtgacag gaacgtcgtc atatcccgtg
     4861 cccatccatc cggcagcagg gcggcaagac gggctttttc tgccatcatc gccatgaatg
     4921 cggcaccttc cttccctgac ggcgcatcgc tggtcagcga ataatgctca cattccaggc
     4981 ggattctggc gggattgctg tacgctccac tgccaaatac attcagacag agtgtccatg
     5041 ccagaagtgc cagggatttg tctgattgct gcatcagtgc cgcctggact gccagcgtgc
     5101 gctctgagga cattttcgtc agcagcggca gactgatttc ctccactgag gctttctcct
     5161 gcacctgttc cgtgcggtaa tcgttatcag ccacatcatc ctcactgcgc aactgcacac
     5221 cacgctggac acacacgttg ccataacgcc aggacaccac cacgccactt ccggcacgca
     5281 tctccggcgt ccacgccctg accttcgcca tgcagcgcat cagcttcatt tcggcttcca
     5341 gcaggtcgga ttcctcacac tggttttcca gcgcatcgta acgtgccatc agttcgttca
     5401 ggcgttcttc ttctgcctcc gtcagcaccg cttccggctc cggcagactg cggtatgttc
     5461 cggcatcctc acggcagaaa ccaacaggct ccatacgtcc ggcgcaccat tcccagcctt
     5521 cggcttcccg aaggtgttcg gcgacagcct ggagtttttc cagcagggcg gcatcgagcg
     5581 ccacgcagtc cacatagcca ccctcgtcgt cactgaacaa atcggtgcgc agttcatctg
     5641 gcgagaaggt atcagccccc acgaagcgga atttactgtt ccctgccacc gccacttcac
     5701 tttcggtcac cagacgacga atggtctgta cttccggttt accgccccat cccgactgac
     5761 aggcggcttc aaacacctgc acctgacgcg cggtgtcgtt ctccagcgcc agcgcctgac
     5821 agtgctcggt ggtgatgcgg tcttctgcca gcgcatcgag gatgacaggc gcaaggtctg
     5881 ccagtttcag cattcgctga acgtggcggg gcgaataacc cagcaaatca ccgatttgtg
     5941 caggtgtttt gccttcctgc gccattgcac ggaatccggc aatctgttcg gcagggtgca
     6001 tatcccgacg atgaccgttc tcggtcatcg atgcggcagt cgccagctcc tgcggaatga
     6061 ccttcacacg gacaggccag tcagccggaa tgatgtcacg ctctgccagc atgttgagtg
     6121 ctgccagtcg gcgaccacct gcggcgacac cgtaacggtc acctggcagg gcatgaacaa
     6181 ccagattctg taacaggccg acacctttaa tggagtccgc cagttcgctg acggactccg
     6241 cagaatacgg caccgtgcgc acattcagcg gtgacttaat cagcgaggcc agcggcacgc
     6301 tcacttcctc cgtctgcgcc agcagggccg acaggactgt ttcctgcgtt tttgcaggtt
     6361 tacggctgga tttacgctcc gtttttgcct tagactctgt aactgacata gttagtacct
     6421 cccaaaacgg taataatgat tgtctgccgg acgcgttccc gtccggcacc tctcttactt
     6481 aataatcccg aaatcacgga tgacatggtt catccagtac ccggcaccgt cctcaaaatt
     6541 ccaggtacgc cagaccattg caccgttatt acggacaacc aggcgaaact gttcgccgtg
     6601 gtcattctcg ataaagacgt tccggtactg tgcggcaacg gcttcggcat gtttgcgggt
     6661 aaaaggaccg tcaggcagtc cctgaagtat tctgaaatat tcgctcatcc tcatctctcc
     6721 ggtgatgccc cgcacaggac ggggcggttg tcacagtcag tcgttcagaa cgggatatcg
     6781 tctgaaaacc cgtaatcgtc accctccggc ggttgcggct gaggctgacc gctgaactgt
     6841 tgcccctctt ccggctgagt ctgagcacct gcggcacgta ccagcatctg catggtgccc
     6901 gtggtcttaa caagaatttc ggtgacgtaa cgggtgatac cgttatcctc ccagctacgg
     6961 gtgcgaagct gaccttcgat gtagacctgc gcgcccttgc gcagatattc acctgccact
     7021 tccgcgagct tgccgaacag caccacgcga tgccattctg tctgctcccg catctccccc
     7081 gtctgtttgt cacgccagct ttctgacgtg gccacctgca ggtttgccac tgcgcccccg
     7141 ttggggatgt aacggacttc cggatctttt cccaggcgtc ctacgaggat gaccttgtta
     7201 atgccacgaa ctgccatgat gtgtctcctt ctgttgatgt tttcagcccc catcatcttt
     7261 tgagtttcgg gagccgcggt tttgacgttt tgcggggacc tgccttttgt tccctccggt
     7321 attcaccatc acctttttga gaaccttcgc cgcaaggggc atcactgcca cggccggaaa
     7381 cgactgtccg ggggcagacc gcaaaacttt ttgcctgctt ttgggcaaaa agttttgcgg
     7441 gatgtgcatt tcacccctgg actggcggag acggttgtgg ctgtctttgc ccccggcgac
     7501 aggggcttaa acaggtgaat ggcaccggac acaacaacgg gcaggcaccc gccgcaacga
     7561 cgtgaagcgc ggtttgcgaa acggcgttgc cccttacatc ccggcggcct tttgccggga
     7621 tgccgggagc gcaggccacg gcgcgacaga ggcggcgcga ccggaacggt cgctgcaagc
     7681 ccgggtttcg cgtcagggat ggaagcccgc cagggcggag acagctccgc tggctccgtt
     7741 cacgacagcc cgccccgcca gggggacgcc cggaaaaggc ataagcagga gaacaacaaa
     7801 acagacagta atcagagcag cagaaaaaag aagaacagta gcagtagcag tagcagtagc
     7861 agtagcagta gcagtagcat gaacaacaac atcatcagta acagacaaat gacaggtctg
     7921 aaaagacaga agcccacagc agaatgtccg cgtaatgtaa ggggcggcgc gtgagcgcgg
     7981 cccctttcag cgccaccggc gcgcggtctt ttcccggcgc gggcagacgg gcatttatcc
     8041 tcccgcctgc ccggagtcat caggcacgca gacgaataat catcgtcgga acatcggtat
     8101 aagcaaacgt gccgcgtggc agctcctcgc ggacgtcaga aaacggtaac agcttctcct
     8161 gctggcgcgg cccgttcaga cagacggcga ccagcacgcc acccggacgc aacagggaaa
     8221 aggcccgcag gatatgccgg atatcctgcc cgtggctgaa cggcggatta atgataaccc
     8281 ggctgtaata ctgcaccggc tgccattcca tgaagtcgcc acactgaacc ctgacaccgt
     8341 taaaattttc ccgcagatac cggaccagcc cgctgttgat ttccaccgca tcacacatgg
     8401 cctccggtgc ggtatcccga atcgcccgca gtatggcccc ggtgccggca gagggttcca
     8461 gaatgtgatc acggttgctg atctccgcca gcgtcaccag gcgatcacag accggggcag
     8521 gagtcacaaa aagctgagat tcagcccgga tttcagcaac cggcgcggcg ttatccagcc
     8581 ttgcgcgcat cagccggata cgttctttta actgactgtg catgtcatta tctccgtcat
     8641 cccccgggga tttcccgggg ggatatacct tatttctgtg ggatttccgt aattttcatg
     8701 tcggtgatat acacattcac cagacggaaa ttattgtcca ttgtgcggcg gtagcggtat
     8761 gccccgtggt cttctgcttc tgccacactg cgcacggcct tacagtcccg gggcagggcg
     8821 gcccactgtg cctttgtcat ttcccggaaa ccttcccccg gatagttgac taccggcgga
     8881 cgcttcgcgg cctggctggc gacggcagcc tcttctgccg atggtgcttt gtagtccgtg
     8941 atgcgatcgg gcgtcacttt catcgtgccg ctgtacccga gaaaactgta attcggcgtt
     9001 gtgactgaac tcaccgcccc gttgcttttg ttcacgcgga tgatggtcag ccactcgccc
     9061 cggctgaaaa cctgtccgcc cggctcaaaa tcctgtgtcc gggtaaccac gccgccgctt
     9121 tcgtccagca tggcacgctc atagttcagg cggttctgat agtggttaac ccagcgttgc
     9181 tgatgctgaa tctgccgttc atgacagcgg atcgcaattt cgcgggcctg ctccgtggtg
     9241 atgatgtcat catccagggc cgaccataat gaccggctcc cttcatactg gcttttttct
     9301 gccgggcgcg gatacgtatc cagcgggaaa caggcgctga tatggtcatg actgctgatg
     9361 agttttgcca tattcagatc cagcgattca gcccgccaca tcgtcagata tttctgcgac
     9421 tgcgcgatgg tcttttcagc cttgcgcaaa tcagcttcga tttttttgat acgacgccag
     9481 cgaacgtccg gacgttcttt atacttcgcg tgaagcagtg ctgaccgccc ccgttcttcc
     9541 cagtattccg cacgttcaaa gagcatcacg gcacgtttca tgccgttttc aatacgctgc
     9601 gcatcacgac gggcgcggcg ttcgctgtga tgccccacaa gaatgggctg accgggcggg
     9661 atcatcgcgg ccagtctttc cacttcatca agtgcctgtg cggattcact ggcccgcttt
     9721 ccgctgtatc cggtaaaccg ctccgcccgt gcttcctgac gttcagcgag cgtgctgtct
     9781 tcatcctcaa tctctccggc aagtgagagg agcacgtctt cccggcccgg tgtccaggcc
     9841 ggcgcgacaa acagcgcctg ttttggtgcc cagcgaaaac cggcatcatg cactttttta
     9901 tacgtctccg ggtcaagacg tgacacggca tacaggcgca gtttgttgtc atccggtgag
     9961 taggtcgcgc ggtacatctg cacagggctg gcggcttccg gggcggtcat ggtgattgct
    10021 gctgatgtca taaaatgttc tccttccgtc tgttgccgcc cctcgcgggg cggctcacct
    10081 catcagttaa aagcgcgttt cagttcgagt gtgcggagca cctgcccgaa agccgtgttt
    10141 ttcatggcgg ggatgtgtac accacgcccc cggtgaaaac tccgggctat gtgtcgcccg
    10201 aaggctttca gcacggggtc aatttccgga gcggggatgt cttcgcgtct gaaacgggga
    10261 tgacggcggg gctttacagc cgctgcggaa ggtgaaacaa tgcttacagt cataaatgga
    10321 ttccttatga caaattggtt aacaatgccg tcaaaattgg tcgcacaatt cttacggcgt
    10381 ggattaagca ggtccctgac ctgctttttt catatctgca tctgacgctt tccggtgttt
    10441 tcctccttct gttcctgcgt tctcattacc tgtcagccgg atgactgaca gaacacataa
    10501 atgcgtattg ccggagcctg aaataaatcc tgatttttaa agaacatcac ccgttgagtt
    10561 tcgggagccg cggttttgac gtttcgcggg aacctgctgc tgtattccct gtggtatgaa
    10621 cttcacccgt ttgagaacct tcgccgcaag gggcatcact gccacggcct gacacacctg
    10681 tcctgggcag aacgcaaaat tttttaccgg gttctgggta aaaaattttg tgggctgttc
    10741 atttaccctg gacagtcagt gcggcaggtt gtggctgtat cagctcccgg cgacagggac
    10801 tcatacgggt gtgtggcacc ctgcaggcaa tgagcaggga cccgccgcaa cgacgtgaag
    10861 cgcggtttgc gaaacggcgt tgccccttac atcccggcgg ccttttgccg ggatgccggg
    10921 agcgcaggcc acggcgcgac agaggctgcg cgaccggaac ggtcgctgca agccagggtt
    10981 tcgcgtcagg gatggaagcc cgccagggcg gagacagctt cgctggctcc gttcacgaca
    11041 gcccgccccg ccagggggac gcccggaaaa gccataagca ggagaggaac ggaaagaaga
    11101 ggaggaagag gaagaggaaa aagaagaaga acagtaacag gcacggtaac cacagaaaaa
    11161 taacatcaga aaaaaacatt ccggacacat cacaaacagg aagggtaacg gacgacgcgt
    11221 cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc aggggttttc gcgcgggctg
    11281 acaggcggcc tccaccgcca gccctgtatc acctgtttct gctgccctgt aagatgtagt
    11341 gaccgcccag gaaaacagcc tgctgattcg ccacaaaatc gcagatttca tcaacaatct
    11401 gcgcggtggt gagcggtttc tgccccattt cacgacgtcg cgcattaatc acggctttgt
    11461 aattgcgcag aatttcgcgg gtttctgtgc tgatattcat gacatgtgct ccccgtccag
    11521 cgcctgtaac acaacgtcca ttgcttcgcg ttcctccctg acgaacgcat caccgtcgcc
    11581 cccgttctca ctcccgacgg catacaggcg cttcatttca ctgcctgcca gcgccatcac
    11641 tttttccagc atcaccgccg gaactgtcac ctgacggctg gcacgctttt cttccatcaa
    11701 ctcacgaaca acgtcgtgaa caatgctgac atccgcacca tattcaaagg catcgtccag
    11761 ccgctgcatg acggtttcca gctcatcgtc tgacaggttc cagtcctggg ctatgtggcg
    11821 cacatcccca cgggtcagca ccacggcaaa aacgcattca ctgtccggca cgtctgtatc
    11881 cagtacatcg cggatgattt ctttaacagt acagtacatg ttctcgtctc ctttacaggg
    11941 gcggcattgc cgccccgtcc gttaatcagt ccagtgccgc gtaaatcgcg ttgcattcag
    12001 ggtgaaattc gatgtgacgc cacaactgcg catcgcgcat ccggtaaagc tgggtcagtc
    12061 ctgcatcacc gctgtcgtgg tacagccaca actggcggtt aatcacaagg gaagtcagaa
    12121 tgatgccgca ggcatcagca ctcacggtac ggtcaaacca gtttgcaccg ttcaccatgt
    12181 ggaaacggtc gccatccggc atcatgtaac cgccaccgtc cggcaggcgg atgaagtgcc
    12241 agaagcctcc ctgatattcc ggcatcattt tttgcgccag cgcgtacaca tacgcctcgg
    12301 catacagaaa atcgttgcca aacaggtaag gcagaaagga gagacggtcg cactcttcaa
    12361 cattcagagt gacaggtttc gcatattgca tgatgttttc ctccgttgtt cacatattcc
    12421 gcggcaccgc ccgcacacga cgctgaggcc acataactga aaaaccacaa ggccatgccc
    12481 tgcatggccg ggtaaagcgg agccagcggc gacttccttt gtgctctgtc cggggaccgg
    12541 caggaaaaag gcggggattt accccgccaa cgtcatcatt tccggatttt tgccagggca
    12601 tccgccatgc tgcggatttc gttccgggct tcccggacca cctcccgcag aacaccacgg
    12661 tcaaaccacc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
    12721 gacaggatat gttcaataca atcgctgacc agatacattt ccaccgccga aaaacgtgcg
    12781 ccccggttca ggatggcatc gagcaccacg ggcaggtttt cgtcgtcctc atcgagccag
    12841 caggggtgag cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagtaac
    12901 gtctgaccgg tggtggtcag cgccgcccgg atacgccgcg tatcttctgc cacccggcaa
    12961 ctgacactgc gtaccgcatc gcgcaccgcc gcaagtacag ccctgatttc gtcttcggtc
    13021 agccattcac cggcatacgg aaaagacacc tgcgttccgt ctgccggaag caccggcagc
    13081 gactgccact gactgccgga cagaacaagt tccgctccgg ttccggtcag tttctgaatg
    13141 gcggaggcca ggtgcggcgg cagtacgccg ccgtggcggt taacgagtaa cgtcgccagc
    13201 cggggcgcat ccagcagaac ataaatgctg atccagcccg gatacttcgg gttacgctgg
    13261 tcgacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtgacgacaa
    13321 atcagtgcgt tcagcgttgt gttttcgttc atgattaatc gttctccctg tatcgccgcc
    13381 cgcaggcggc gataacatca gccgttttcg tgtgtgttgt ttttcagcag gatgcgggcc
    13441 tgattaacca ccgcctgacg gcgggtaacg cattccggga cggcgagacc gtaatcacgg
    13501 gcggcccatt cacgtttcca cagtgcctgt tcggcggtct gaatcaggga cgccagcagt
    13561 tcagccggga cggtcacctg gcggttttcg ctcacgttgc tgataatctc catcgccgcg
    13621 gcagaagaaa taccggactc aattcgctgg tcttccggga tgtcctccag gcgcgccagg
    13681 acggtcctga tttcaggatc ggacagggaa atatccattc cgtcagcgag ggcctgaatc
    13741 tcttccgggg accagataac cagcatcagc ggtgtatatc ccggatactt tgctgccagc
    13801 tcccggcata gcgtttcgca tgttccgtac attctcgtct ccttctcaat ctttaaagca
    13861 cgcatccagt tccagaattt cggtatacgc ctccatgaac aggcgattgg cgcgatacag
    13921 cgtctggcgt gccaggcgtt caacaagcac atcggaatca aagcgacagc gatgctccat
    13981 atcgttcagc ctggcccgaa ggtcacacat ggtgttggcc acctccgcca cgtcggcgcg
    14041 aagtttttca tgtccggcat agttcattta cgcagcctcc ggcataaacc agtcatcatt
    14101 tgcggccccc tgctgcatgg cccgctgtac ggcagccaga cgttgctgtc cggcacggtg
    14161 atactgttca agcagctcga taccgatata ccggcgtccg gactgaaggg cggccacaca
    14221 ggttgagccg ctgcctgcaa acgggtccag cacaatcgcg ttcgggtgtg tgaagctctc
    14281 aatcagtggt tgcaggctgg taacaggctt ttccgtcggg tgatgacgat tgcctgaata
    14341 tttccagccc agcacgtcgg gcaatggctt ctgaggcagc gccggacgcc cttttgccag
    14401 gatgtaagcg cattcgtggc gatagcccac atatgcggcc ttcgaagtgt aggttttggt
    14461 aaacaccagg tggccaacca cgctgaatcc cgcatttttc caggcggcca taaagcgatc
    14521 gacgcggttc cagccataga agctcaccat cagcgcatct tttttcagca cgcggtacat
    14581 ttcattacag gccggttgca gccattcatc ggttttatcg ccggcgatgg tacgcccctg
    14641 acggtcacgg aaaccgacga gatatggcgg gtcggtgagg atgaaatcga caccattgcc
    14701 cggaatcgtg gccataacac gcacgcagtc accctggata aaacgggaca taacagcact
    14761 ccttctctta cagagggcac cgggagggcg cccctgtggc gcccgttccc cgcggcctgt
    14821 gggctgttca ccgcgtccgg taacgggtca ggccgaccgt ggaatatcgc agccgtcgtc
    14881 tttttgacgg cgagaatatg ccgcgaaagc ggcttgaagg gcgttcatca gaaaagggcg
    14941 acgggctggc gtacactgtc accgatgcca gccgggagcg gtaaaagcgc ccgtcccgtg
    15001 cccggccgga cgcgggacac tgaccacagg cagcgttcct gccgcgccgc gtcagccggc
    15061 gcagtaaaaa gaggagcccg gcgacatcct ttaccggaaa agaaacgggc ggttgcccgc
    15121 ccgtctccgt catacttcat ccaccgtaat gcgccacctt gtcgactatc cactccggcg
    15181 cggtgacttc cggcagctca tcatcatctg tcggggaaga ccattccagt tccccccaca
    15241 gcacatcaac aagctctcca cgctcatatc gctgccagcc acagaaattg cagccctgcc
    15301 cggcgtacca gtgctccagc atgcaactga aacggcggct taatactgca ataacgtcgc
    15361 tttccggctg acaccacggc gtgtcgaaat caacctgaat gaagttgtct ccctgactgc
    15421 tgatattcac ctcataccca caaggccact tcacgccata ctgttccgta taccagtgat
    15481 atgcagaagg aacaccgttc agaacaccac cgttaaagcc attcacttca acatccaggc
    15541 gagtggacag aaccgtcagc aggtcaaacg gcatggtttt ttccggcagg acgttgtcac
    15601 acagacggtt ccaccacaca gatacatcct cgttactcca gatgtcgcac cagtcacctc
    15661 tttttggggt gaaaagagcg gtaatgcttt ccctggcatc atccggtaat tcttcccagc
    15721 gacgccggcc agtaccgctc tgcagccaga gttcatgcag catcaggcag ttttgctcat
    15781 ccagaagtac accgtcctgc aggtgtgtca gccagcgggt gaacgcgata ttctccggcg
    15841 atacaacgcc acgcccggca gccgtcagtc cggggcatgg ttcaaaccgc acatcttcag
    15901 tggtctgcag aagtccggca cttccggcca gaaacagctg aataccttca tttgtggcgc
    15961 ggcgataaag cggtgtgact gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg
    16021 gttcaccaga aaaatacata cgatcgggta gcagtgatgc attactgcat caccgccccc
    16081 taagaaccgt acgtgccagt ttcccggcat acggctcaag cctttctaag cccactctca
    16141 tgagccggtt tcactacatg tatacctttc gcatgaacaa gtgtgtgaca catcggatgc
    16201 agcataatca aattggagag gcagttgctc cctccatcga ctcgccgtat gatgtgatga
    16261 acctgccact gggaatcaga tgtaatcctc tcatcacata tggggcatcg gccccgctgc
    16321 tcaaaccaga tttgaatgag ctttttccgt ccttgcagtt tgtgccgcat tttgtctgca
    16381 caacgctcct caaagtacat ctcccattga ggatcgaacg gattagctgc aattttaatc
    16441 ggtttaaacc ggttgatagg cgtatcgatt gctttcctca gatttgcata ctgaggctta
    16501 ccgttggcaa ggagttctcc ggtagccgtg gagaaagacc agtttcgggc accttgttga
    16561 tggaaatatc ttttccgaat ccacttcttt cccttattgg gatgacgccg aacagcccaa
    16621 cgccataaag ccagccagat ctcattgtca actttgttaa atgcgacctt ggccacaatg
    16681 tgttgatggt aagccgccca ccctttgatc attgggttca gcatgcggat caacgtaagc
    16741 tgatccatag ctttgttgcc tttgattaca gcgcgtattt tctttaaaaa ggaactgaca
    16801 ttggctttag agggtttgat cagcatctta ccgccatatt tgcgaatgtt ctgtccaaga
    16861 aaatcaaatc cttcctcgat atgtgtgatt ttcgtctttt ccggagaaag cattaatcct
    16921 ctttcagcca taaaccgcct gacgacgggt aatacctgag actcaagaag ttcttttgac
    16981 tctccagtga taataaaatc atcagcatat ctcacatagt taactttcgg gttaaccaat
    17041 cgtcccttga ctgttctctt tttaaactcc gaagcaagaa gcgcctccag tccatccaga
    17101 gcaatgttag ccagtacagg agaaattatt cccccctgag gagttcctga atttacaggg
    17161 aataactgcc ccttttccct gtaacccgct ttaagccact ttgtcaggat ttgtctgtct
    17221 gttgaggtgt tatcgattag ccattgatga gatatattgt caaagcatcc ccgtatatcc
    17281 ccctcaagca cccattcagc ggatgttttc tgcgcaagaa cacagaagca ctgtgctatc
    17341 gcatctgcag tagaccttcc agtccgaaag ccgaaggatc tctggtcagc agtgacttca
    17401 gcaacgggct caagagccag caaatacaac gcctgcatcg cccgatcctt catagtgggt
    17461 atcccaaggg gacgagactt cccatttgac ttaggtatat aaacccgctt aagtggctgt
    17521 ggtttgtaac cacgccgttt cagcaggtta atagcaagaa atttaacctc cggggaagac
    17581 catgtctgac catcaacccc aggagtacgt ttcccgctat tctctgtaac tcgtctgaca
    17641 gcaactgctc tgccactaaa cgagtgagtc agtatccatt gcaaggattt gaccttgcca
    17701 tgtttacctt ctcgtgttgc ctttacgata cgcgcttgca gtctccgcac ttcccgatag
    17761 cactgacgcc agttgatact gtgccaggag tcaccgttaa gtaaggacgc acctttacag
    17821 gttgtagcct gctcagtcat ctgcttttcc tttctggaag attactcaaa ctctctcgta
    17881 atgaaagacc cagtggaagt ctgcccgctt tcgcgtaggg taatgttgcc gggctcaacc
    17941 cttatccggt ccattacaga ctggcattcg ctttttccac cttcttttac ccgcaccgcc
    18001 aacagtatcc cttgcggtta acctgcctca cgaggcagcg atacgggctt accatgttcc
    18061 acctgaataa caacagtagg ttagactctg ctcacaacac cggtggttta ttgtccgtgt
    18121 acccctagca aacagaggag taaccaacca catacctttt ggttcaagcc tgtcagcatc
    18181 tttggcttgt tctggttaac gacgcttcag cagttcacat atgttagtca tgctaccgag
    18241 cctagacctc ctccgcctga tgctggcaga ttacatgtaa cctcacggtt tcatgcgatc
    18301 cgaagatccg gtttcattgt cctgacagct tcacacccca ccgttaccag tgacgcatgt
    18361 gtcagtaggc tactgatgac ggaacatcag gttccctgac ggaatcgaac tccgctggaa
    18421 caattattca ggcgacttca tgtcgcacat tcgagcacca gtttggcatg ggaattctcc
    18481 tctcacatgc ggcctccatc aggccgtgcc gcacctgtaa ccgggcgacc gcatacgaat
    18541 gcaggtgcgg ccccgaagcc gcagcgcgtg gaggaggaac gacgaaacgt gaccgggtga
    18601 ggaggccggc gacagcgaca cacttgcatc ggatgcagcc cggttaacgt gccggcacgg
    18661 cctgggtaac caggtatttt gtccacataa ccgtgcgcaa aatgttgtgg ataagcagga
    18721 cacagcagca atccacagca ggcatacaac cgcacaccga ggttactccg ttctacaggt
    18781 tacgacgaca tgtcaatact tgcccttgac aggcattgat agaatcgtag tctcacgctg
    18841 atagtctgat cgacaataca agtgggaccg tggtcccaga ccgataatca gaccgacaac
    18901 acgagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg ggaccgtggt
    18961 cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc tgattatcag
    19021 accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt tatgctttct
    19081 ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac gtggtcgcat
    19141 cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat acactcagtt
    19201 ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa tactgtcgct
    19261 ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga cgttttaagc
    19321 acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc ttttttctgc
    19381 tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa ggctttttga
    19441 agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc aacaactgat
    19501 ttaggcaatt tggctgtgtt gatacagcgg gtaataatct tacgtgaaat attttccgca
    19561 tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc ataacgctga
    19621 ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa tgcagccatc
    19681 tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag tgcagcagct
    19741 ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg accgaacgcc
    19801 ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc gtcctcagta
    19861 agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc aacactatca
    19921 ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa agtaatggca
    19981 ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg ggcagctggt
    20041 gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg aataacaggc
    20101 gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa atttcattgc
    20161 agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag cgttgatcaa
    20221 tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca tccgtttcac
    20281 gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc tccatccacg
    20341 gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga
    20401 accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg
    20461 aggtgtagtc aaacaactca gcaggagtgg gaacaatcag cacatcagca gcacatacga
    20521 cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca tcatagtcat
    20581 gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc agtttacctt
    20641 catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag gaaggaataa
    20701 tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc ttttccccaa
    20761 gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc catccgtgat
    20821 acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt agccccttca
    20881 gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg
    20941 cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca
    21001 catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca acccgtcctc
    21061 gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg atagcctgag
    21121 aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt attttcctcg
    21181 cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag
    21241 cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt taatcattgc
    21301 tttgcatttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc
    21361 atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag ataagaagag
    21421 cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac cgagcatagc
    21481 gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt acgctcagcg
    21541 caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc ggatagccaa
    21601 ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta acccccgata
    21661 tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg ccctcaaatt
    21721 tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa
    21781 cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga accaccctca
    21841 aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat cgcggaagga
    21901 aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga ggcgcattgg
    21961 agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga agtcaggcat
    22021 acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcaattt tcagagagac
    22081 gatgcctgag ccatccggct tacgg
//
LOCUS       DAZFKY010000071        21831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFKY010000071 DAZFKY010000000
VERSION     DAZFKY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..2774
                     /gene="stcE"
                     /locus_tag="ACHKCL_002357"
     CDS             78..2774
                     /gene="stcE"
                     /locus_tag="ACHKCL_002357"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO5763316.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2861..3736
                     /gene="gspC"
                     /locus_tag="ACHKCL_002358"
     CDS             2861..3736
                     /gene="gspC"
                     /locus_tag="ACHKCL_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5763317.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3737..5704
                     /gene="etpD"
                     /locus_tag="ACHKCL_002359"
     CDS             3737..5704
                     /gene="etpD"
                     /locus_tag="ACHKCL_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO5763318.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5704..7209
                     /gene="gspE"
                     /locus_tag="ACHKCL_002360"
     CDS             5704..7209
                     /gene="gspE"
                     /locus_tag="ACHKCL_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5763319.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7211..8434
                     /gene="gspF"
                     /locus_tag="ACHKCL_002361"
     CDS             7211..8434
                     /gene="gspF"
                     /locus_tag="ACHKCL_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5763320.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8465..8899
                     /gene="gspG"
                     /locus_tag="ACHKCL_002362"
     CDS             8465..8899
                     /gene="gspG"
                     /locus_tag="ACHKCL_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5763321.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8896..9450
                     /gene="gspH"
                     /locus_tag="ACHKCL_002363"
     CDS             8896..9450
                     /gene="gspH"
                     /locus_tag="ACHKCL_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5763322.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9447..9812
                     /gene="gspI"
                     /locus_tag="ACHKCL_002364"
     CDS             9447..9812
                     /gene="gspI"
                     /locus_tag="ACHKCL_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5763323.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9809..10408
                     /gene="gspJ"
                     /locus_tag="ACHKCL_002365"
     CDS             9809..10408
                     /gene="gspJ"
                     /locus_tag="ACHKCL_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5763324.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10405..11382
                     /gene="gspK"
                     /locus_tag="ACHKCL_002366"
     CDS             10405..11382
                     /gene="gspK"
                     /locus_tag="ACHKCL_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5763325.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11316..12593
                     /gene="gspL"
                     /locus_tag="ACHKCL_002367"
     CDS             11316..12593
                     /gene="gspL"
                     /locus_tag="ACHKCL_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5763326.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12580..13092
                     /gene="gspM"
                     /locus_tag="ACHKCL_002368"
     CDS             12580..13092
                     /gene="gspM"
                     /locus_tag="ACHKCL_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO5763327.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13213..13983
                     /locus_tag="ACHKCL_002369"
     CDS             13213..13983
                     /locus_tag="ACHKCL_002369"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5763328.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14075..14476
                     /gene="eptO"
                     /locus_tag="ACHKCL_002370"
                     /gene_synonym="gspS"
     CDS             14075..14476
                     /gene="eptO"
                     /locus_tag="ACHKCL_002370"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO5763329.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14655..15826
                     /locus_tag="ACHKCL_002371"
     CDS             join(14655..14904,14904..15826)
                     /locus_tag="ACHKCL_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5763330.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            16367..16882
                     /gene="ehxC"
                     /locus_tag="ACHKCL_002372"
                     /gene_synonym="hlyC"
     CDS             16367..16882
                     /gene="ehxC"
                     /locus_tag="ACHKCL_002372"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO5763331.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            16884..19880
                     /gene="ehxA"
                     /locus_tag="ACHKCL_002373"
     CDS             16884..19880
                     /gene="ehxA"
                     /locus_tag="ACHKCL_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO5763332.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            19930..>21831
                     /gene="ehxB"
                     /locus_tag="ACHKCL_002374"
                     /gene_synonym="hlyB"
     CDS             19930..>21831
                     /gene="ehxB"
                     /locus_tag="ACHKCL_002374"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO5763333.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATS"
BASE COUNT         5770 a         4453 c         5883 g         5725 t
ORIGIN      
        1 tataaaaata accaacgact agtgaataag tcgctcctga aaaaataaaa tatagaaata
       61 ctgttatatc cggctgcatg aacactaaaa tgaatgagag atggagaaca ccgatgaaat
      121 taaagtatct gtcatgtacg atccttgccc ctctggcgat tggggtattt tctgcaacag
      181 ctgctgataa taattcagcc atttatttca atacctccca gcctataaat gatctgcagg
      241 gttcgttggc cgcagaggtg aaatttgcac aaagccagat tttacccgcc catcctaaag
      301 aaggggatag tcaaccacat ctgaccagcc tgcggaaaag tctgctgctt gtccgtccgg
      361 tgaaagctga tgataaaaca cctgttcagg tggaagcccg cgatgataat aataaaattc
      421 tcggtacgtt aaccctttat cctccttcat cactaccgga tacaatctac catctggatg
      481 gtgttccgga aggtggtatc gatttcacac ctcataatgg aacgaaaaag atcattaata
      541 cggtggctga agtaaacaaa ctcagtgatg ccagcgggag ttctattcat agccatctaa
      601 caaataatgc actggtggag atccatactg caaatggtcg ttgggtaaga gacatttatc
      661 tgccgcaggg acccgacctt gaaggtaaga tggttcgctt tgtttcgtct gcaggctata
      721 gttcaacggt tttttatggt gatcgaaaag tcacactctc ggtgggtaac actcttctgt
      781 tcaaatatgt aaatggtcag tggttccgct ccggtgaact ggagaataat cgaatcactt
      841 atgctcagca tatttggagt gctgaactgc ctgcgcactg gatcgtgcct ggtttaaact
      901 tggtgattaa acagggcaat ctgagcggtc gcctaaatga tatcaagatt ggagcaccgg
      961 gtgagctgtt gttgcataca attgatatcg ggatgttgac cactccccgg gatcgctttg
     1021 attttgccaa agacaaagaa gcacataggg aatatttcca gaccattcct gtaagtcgta
     1081 tgattgttaa taattatgcg cctctacacc taaaggaagt tatgttacca accggagagt
     1141 tattgacaga tatggatcca ggaaatggtg ggtggcatag tggtacaatg cgtcaaagaa
     1201 taggtaaaga attggtttcg catggcattg ataatgctaa ctatggttta aatagtaccg
     1261 caggcttagg ggagaatagt catccatatg tagttgcgca attagcggca cataatagcc
     1321 gcggtaatta tgctaatggc atccaggttc atggtggctc cggaggtggg ggaattgtta
     1381 ctttagattc cacattgggg aatgagttca gtcatgaagt tggtcataat tatggtcttg
     1441 gtcattatgt agatggtttc aagggttctg tacatcgtag tgcagaaaat aacaactcaa
     1501 cttggggatg ggatggtgat aaaaaacggt ttattcctaa cttttatccg tctcaaacaa
     1561 atgaaaagag ttgtctgaat aatcagtgtc aagaaccgtt tgatggacac aaatttggtt
     1621 ttgacgccat ggcgggaggc agccctttct ctgctgcaaa ccgtttcaca atgtatactc
     1681 cgaattcatc ggctatcatc cagcgttttt ttgaaaataa agctgtgttc gatagccgtt
     1741 cctccaccgg cttcagcaag tggaatgcag atacgcagga aatggaaccg tatgaacaca
     1801 ccattgaccg tgcggagcag attacggctt cagtcaatga gctaagtgaa agcaaaatgg
     1861 ctgagctgat ggcagagtac gctgtcgtca aagtgcatat gtggaacggt aactggacaa
     1921 gaaacatcta tatccctaca gcctccgcag ataatagagg cagtatcctg accatcaacc
     1981 atgaggccgg ttataatagt tatctgttta taaatggtga cgaaaaggtc gtttcccagg
     2041 ggtataaaaa gagctttgtt tccgatggtc agttctggaa agaacgtgat gtggttgata
     2101 ctcgtgaagc gcgtaagcca gagcagtttg gtgttcctgt gacgaccctg gtggggtatt
     2161 acgatccgga aggcacgctg tcaagctaca tctatcctgc gatgtatggt gcctatggct
     2221 tcacttattc cgatgatagt cagaatctat ccgataacga ctgccagctg caggtggata
     2281 cgaaagaagg gcagttgcga ttcagactgg ctaatcaccg ggctaacaac actgtaatga
     2341 ataagttcca tattaacgtg ccaacagaaa gtcagcccac acaggccaca ttggtttgca
     2401 ataacaagat actggatacc aaatcgctca cacctgcgcc agaaggactt acctatactg
     2461 taaatgggca ggcacttcca gcaaaagaaa acgagggatg catcgtgtcc gtgaattcag
     2521 gtaaacgtta ctgtttgccg gttggtcaac ggtcaggata tagccttcct gactggattg
     2581 ttgggcagga agtctatgtc gacagcgggg ctaaagcgaa agtgctgctt tctgactggg
     2641 ataacctgtc ctataacagg attggtgagt ttgtaggtaa tgtgaaccca gctgatatga
     2701 aaaaagttaa agcctggaac ggacagtatt tggacttcag taaacctagg tcaatgaggg
     2761 ttgtatataa ataatcctga gacggtgcgg ccacttcggc cgcattagta acacattcgt
     2821 cccgttaatc aaaacataaa gtagcgtcca gcgttttatt atgttgttct ttctatcatt
     2881 ccggggtgac cggggcttat ttataaaaga tatcgtactt aaaatgctga cgccaaaccg
     2941 gctattgtgc gtaattttac ttattgccgg atatcagctg gtgtcggtta tccatcattt
     3001 ctggctgact caggcagcat cggtgcccgg cttatcccgt gttagcgcgc cggaaacagc
     3061 ggtaactggt gatcaaactg aagaacgttt tgtttttacg ttattcggca gggcatcccc
     3121 actatcatcg gaggggagag cgcaggaaac aatgccttcc ctgtcagatg atctgctttc
     3181 aggggaggat cttgacgtga gaggtatact ttatagttcg gttgcagagc attccgttgc
     3241 catatttgca cataataaca gacagttcag tctgagcgtc ggcgaaaaag tacccagcta
     3301 cgatgctacg attagtgcta tttttagcga tcatattgtt attaactatc agggaaaaac
     3361 tgtatcactg cctctgcgat atgataatac cgaaaaaaag aatgcatatg acaataataa
     3421 tttaacagtt ggagacgtga taacccaaga taattttcgg gtagaaagtg tttttgatat
     3481 tatgagcttt tcagccgtta cggttaataa tacattaagc ggttatcgct tgattccggg
     3541 taagcacagt tcgttatttt ataatgctgg gttgcatgat aacgatctgg ccgtatcggt
     3601 taatggttca gaattgcgtg ataccagaca ggcgcagcag ataatgaagc aattgccaga
     3661 acttaaagaa ataaaaataa ccgtcgagcg tgatggtcag ttatatgatg catttattgc
     3721 tgtaggagaa aactgattgt tcaggaaatg gttgaatggg cgtttgccgg tacttgtgtt
     3781 cactacagta attttggggg ccattccagg gtggggggct gaattttcgg ccaactttaa
     3841 agatacggat attcaggagt tcataaatac tgtcagtaaa aatttacaca aaacggtaat
     3901 aattaatcct gacgtgcagg gaaccatcac tgtacgcagc tacgatatgc tgaacgagga
     3961 acaatattat cagttctttc tcagtgtgct ggacgtttat ggttttgctg tggtcgatat
     4021 gcacaacggt atactgaaag tagtgcgctc aaaagatgcc aaaacgtcgg cggtgccggt
     4081 agctagtgat gtcagtcccg ggactggtga tgaggttgtt acccgggtgg tccccgtaag
     4141 taacgtggca gccagagatc tggcgccttt gctgcgtcag ctcaatgata atgctggcgc
     4201 aggaagcgtg gtgcattatg aaccttctaa tgttttgttg atgaccggac gtgctgcagt
     4261 gatgaaacgg ttgatggaga ttgttgaacg tgtggataag gtgggtaatc gcagcgttgc
     4321 cacggtcccg ctcacctacg cgtccgcaac agacgtagcc agacttgtta cggaactgac
     4381 taaagaaaca gataagacag ctatacctgc ttggatgacg gcgaaactgg ttgcagacga
     4441 gaggacaaac tcagtgctcg tcagcggaga gccaatctcc caacagcgta tcatctccat
     4501 aattaagcaa ctggatcgtc aggaggatgt tcagggtaat actaaggtga tttacctgaa
     4561 atatgcgaag gcgaaggatt tagtggaagt cctgacaggt atcagcagca gtattgaaaa
     4621 cgactctaaa aagagtccgt caacggaagc cttgcgcaaa ggagtgacga ttaaatccca
     4681 cgaacaaacc aatgccctga tcctgacggg ggcccctgac gtcatccgcg accttgaaaa
     4741 tgtgatttcg cagttggata ttcgtcgtcc tcaggtcctg gtggaggcca tcattgctga
     4801 aatacaggat gctgacgggc tgaaccttgg gatccagtgg gtgaataaac atgccggtgt
     4861 ggcgcagttc accagtaccg gtttacctat taccacgatg gttcagactc gtcagaacga
     4921 aatcttagac agcgatcaga gcaatgccct gagcatgttt aacggaattg cagcggggtt
     4981 ttatcaggga aactgggcga tgctgttgac ggcgctctcc acaagtagca agaatgatat
     5041 cttggcgacc cccagtattg tcacgctgga caatatggag gccactttca atgttggtca
     5101 ggaggtcccg gtactttcgg gctcacagac aacctctggg gacaatattt ttaacacggt
     5161 cgagcgcaaa acggtgggga tcaaactcag ggtaaaaccc cagatcaacg agggtgattc
     5221 cgtgttactg gagatagaac aggaggtgtc cggtgtggcg gacactgcag tagccaccac
     5281 tactgacttg ggagcaacct tcaacacccg aacagtgacc aatgccatgc tggtcgggaa
     5341 tggcgaaacg gtggtggtcg gaggattact ggataagtcg atcaggggga gtgagagtaa
     5401 agtgccactg ctgggggata tcccggtact ggggcatctt tttcgcgcaa aaagcgaaca
     5461 gacagctaag cgtaatctga tgctgttcat tcggccaact attattcgtg agcgcgacgg
     5521 atttcgtcat gcttcggccg aaaaatacca gtcgtttaat caggaacagg tgcagtcgcg
     5581 tggcaaagaa acaacggcgc tgacgctgaa tgaggaacag ctcaggctgt cccccgatca
     5641 agacgatacg gctttccgga aggtgaaagc ggcgattgct gcgttttatg cgcaggagat
     5701 gtaatgagca gagtagtaca gaatgtcagt gaatcacgtc cccttctgcc tttttccttt
     5761 tcccgtacgc agagaatttt gttgctgcgt gaacaggagg ggaatcgagt gttctgcatg
     5821 gaggacacac ctgccagtgc cctgttggag gtgcgcaggg tggctgaagg tcctctgaat
     5881 gtcacaacag tgtcggcgga agcgtttgaa aaacagctgg tgagcagcta tcaacgtgat
     5941 tctgatgaag cgcgacaaat gatggctgag attggtaatg aaatggattt ttataccgtc
     6001 gcaggggagt tgcctgaccg tgaggactta ctggatgcga atgacgatgc gccaattatc
     6061 cgcctgatta acgcaatgct gacagaggca attaaagaga aagcctcaga tattcatatt
     6121 gaaacttacg aacgccatct gcaggttcgc tttcgcattg atggcgttct gcgggagatc
     6181 ctccgtttac ataggaatct ggcttcgtta ctgatttcac gcattaaagt catggcccgg
     6241 ttggatattg ccgaaaaacg cgttccccaa gatggccgca tggtactgcg tatcggtggt
     6301 cgggcggtgg atgtgcgtgt ttcaacgttg ccttcaaatc atggtgaacg catcgtgctg
     6361 cgtttactag acaaaaatag cgttagtctc gatcttgctg cactcggcat gtcgcagcag
     6421 aatcagcgac acattgatgc actgatccgc cgtcctcacg gcattatttt ggtgaccggg
     6481 cctacagggt cgggtaaaag cacgacgctt tatgccgcgt taagcctgct gaatccccga
     6541 gaccgcaaca ttatgacggt cgaggatcct gttgaatatg aactggacgg tatcagtcag
     6601 acccaggtca acccgaaggt ggacatgacc tttgcccgga gtctgcgcgc tattttacgt
     6661 caggatccgg acgtggtgct tgtgggggag atccgtgacg gtgaaacagc ccagattgct
     6721 gtgcaggcct cgctgacagg gcatttggtg ctgtcaacgt tacatacgaa tagtgcagca
     6781 ggcgcgctgt cgcgtctgca ggatatgggc atccctcctt ttctgctttc cacctcatta
     6841 ctggctgttc tggcccagcg actggttcgc acgttgtgtc cgcgctgtcg tcagccctgt
     6901 caggtcagta ctgagttagc gatggacatg gacattcccc ctgaaaccac gatctggcag
     6961 cctgccgggt gtcagcactg cagtttcacg ggctatcacg ggcgcaccgg gatccacgag
     7021 ctgttgctga ttgacgatcg cattcggacg gcaatctatc agggggaggg ggagctgggt
     7081 attacccgct tggcagggag tcgctatctg acgctgcgtg gcgacgggcg acaaaaggta
     7141 ctagccggtg agaccagttg ggaggaggtg gttcgcgtta ctgaaagcag attgcaggaa
     7201 gaggaatgat atggcgttgt ttcattatca ggcatcagat attcacggta gaaaacgcag
     7261 tggcattctg gaggcggatt cggcccgaca tgcccgtcag ttgctgcgtg agcaggcgct
     7321 tattccagtc aggttggatg agaaacaggt ccatcacaag cactcactgc ggagtatcct
     7381 gaggtttcgt ccgcgcgggg ggagcagcgc cgaactcgcg ttactgacac ggcagttagc
     7441 cacactagtg gccgcatctc tgccgcttga agaggcgctg gatgcgttgt tgaggcagag
     7501 tgaaaaaccg cgtcagcgga acttaatcgc tgccgtgcgc accaaggttc tcgagggaca
     7561 ttccttggcc gcggcgatgg gcatgtttcc aggtacgttt gagcgtcttt attgtgcgat
     7621 ggtggcggcg ggggaaacat ccggtcgttt ggacgtggtg ctcagtcgtc tggctgacta
     7681 taccgagcag cgccagatca tgcgaaaccg tctactccag gcattgctct atccttgtgt
     7741 tctgacgttg gtggcggttg gggttattgc cattctgctt actgcggttg tgccgaaagt
     7801 ggttgagcag tttattcata tgaaacagac ccttccctta tctacccgcg tactgatggg
     7861 ggctgctgag gtgagccaga cttggggccc gtggctgtta ctggccgcag cgctgggcgg
     7921 gatagcgggg cgcatgatat tacatcagcc ctcccagcgt ctggcttttc atcatctgct
     7981 gttgcggctg ccggtcgtgg ggcgcatatc gcgcggtctg aataccgcac gttatgcccg
     8041 cacgctgagc attcttaatg ccagtgcggt accgctgctc caggcgatgc acatcagtgg
     8101 tgacgtactg agtaatgact gggcccgtca tcagttagct accgcggccg agttggttag
     8161 ggaaggggtc agcctgcatc aggcactgga gcagacttcg ctgtttccgc ctatgatgcg
     8221 gcacatgatt gccagcggtg aaaatagtgg cgaactcgac agcatgttgg aacgggccgc
     8281 cgacaatcag gatcgtgagt tcagcacaca gatgcaactg gcgctgggat tgtttgaacc
     8341 gctgttggtg gtgggtatgg ccggggtcgt tttgtttatt gttctggcaa ttctgcagcc
     8401 gctcctgcag ctcaacaaca tgatgaatat gtgaatcctt ctttaacagt caacggagaa
     8461 aaacatgcga aaacagcatc agcgtggttt taccttgctg gaaataatgg tggtaattgt
     8521 gatcttggga gttttggcca gtctggtggt cccgaatctg atgggaaata aggataaggc
     8581 cgatcggcag aaagtgatga gcgacttggt tgcgctggaa agcacgctcg atatgtaccg
     8641 gttagataat aaccggtatc cgacaacgga gcagggactg cgggcactgg tcagtaaacc
     8701 caccgtgcaa cctgaacccc gtaactaccg gcaggatggt tatatccgcc gtttgcccca
     8761 ggatccttgg gggggggatt atcagctgtt aaatcctggt cagtacagcg acattgatat
     8821 cttttctcct ggcccggatg gtgtgccaaa cactgaagat gatattggta actggacgct
     8881 cggtaacgcg cagccatgag tcagcgcggt ttcacaatac tggagatgat gctgatcctt
     8941 ttgttgatgg gcatcactgc agggctggta ctaatgtcat ttcctgactc tgctcagaat
     9001 catctgcaac aacagcggga gcgtctgcag gcgcagctgg attatgcctt ggatagaagc
     9061 cagcaggacg gcttgctgat ggggattcag gttcggtttg acggatggaa atttaaggtt
     9121 ctgcagcgcg gaacagcgga aagccccccg acattagctg agggagggga tatttggcag
     9181 gggtatgtct ggcaaacttg gcaaccgcga cgtgcagcca tggggggcaa acttccggat
     9241 gatgtgcgtc ttgagctgca gtatctcaga ggtttgcagt ggatgtctga gcatgatgac
     9301 ggggctgaac ccgatatttt gctgcttccc ggaggtgagg taacgccgtt cagacttctt
     9361 ttcaggcagg tcggagagga ggccgttgtc gggctgcagg ttgatgagaa cggtttgatg
     9421 actctttttg aaggtgaggt ctcattgtga aggggcaaga aggaatgaca ttactggaag
     9481 tcatagtagc tctggttgtg tttgcgctgg caggaatggc gctgatgcag gcctcgaccc
     9541 agcaggctgc ggggatcgga cggatggaag agaaggtgct ggcaggctgg ttggccgata
     9601 accagatggt gcaattgcaa ctcgagaaaa cttggccgga aaatggctgg ggcgagaaga
     9661 cgatcagctt cgctggcacc gaatggtatt tacgctgggg cgggccggac agcgacgtgc
     9721 cgcagccgcg ctccttagag gtagaggtac ggcgcactaa agaggagacg tccgccttgg
     9781 tttccttgcg tagcagtgtg gttcgtgaat gagtcagcaa cgggtaaaag gttttacgct
     9841 actggaaatg ctgttggcac tggctgtttt tgcagcgttg agcatcagcg catttcaggt
     9901 gctgcagggt ggaatacggg cgcacgagct gtcccgggat aaagtacagc gtctggctga
     9961 gctgcagaga gggatcagcc agatggagcg agatcttacg cagatgctcc caaggcacag
    10021 ccgggggaat gaaggcctgc tgctcgctgc acctcatctg ctaaaaagtg atgactgggg
    10081 gatttctttt acgcgaaaca gctggctgaa tcccgccggg atgctgccgc gcccggagtt
    10141 acagtgggta ggttatcggt tgcgacagca aaagttagaa cgcctgagct actttcatgt
    10201 cgatcaccct tccggtgttt ctccggatgt cagggtgatg ctggacggcg ttcacgcatt
    10261 tcgtctgcgt ttttttgtta acggtgattg gcaggcgcgc tgggacagta ccggcatcct
    10321 gccgcaggct gttgaagtga cgctggttat ggatgatttt gctgaactgc cccgtctgtt
    10381 tcttgtgagc aaggagaccg ctgaatgaaa ttgcgggaac agggcgtggc gcttctggtg
    10441 gtattgctga tcctgtcgtt aatggtaacg atagcggcgg tgattgcgga gcgcaacggt
    10501 cggacatttt tgcgtacagt agcgcagttg gatcagctgc aggcaaagtg ggatggctat
    10561 acggcagaga cgatagcaaa acaaatctta cagaggagca ggcaggagtc gccccggaaa
    10621 acgcatctgg cacaaaattg ggcgcagtca gaacgacagt ttgagaccag agggggagat
    10681 gtgcgggggc agatcgtgga tgcgcaggcc tgttttaatc ttaatgctat taattatgga
    10741 gtggtggatc tgaccagtat accttatgcc gccagaattt ttcagcagct attaatcaac
    10801 ttgcaggtag agcttttaca ggcccggcaa gtgacggcag ccctacgtga ctggatcgat
    10861 cgtgatgata agccggtaag gggcggtgct gaagacgagg tgtatatggg gatggaacct
    10921 ccctatctgg cagccaacca gccgatgcag gacgtcagtg agttacgcct tatccgggga
    10981 atagatgcca ggctttaccg caaacttttg ccgtatgttt gtgtactgcc tacgtcagat
    11041 ctgtctgtga atgtgaatac tctgctggat tcacaggcgc cgctgctggc ggcgctcttt
    11101 ctgacgaaac cggacagtct tccggtgacg gaattactgc agcggcgacc ccgtactggt
    11161 tgggagagcg tggcggcgtt tcttgacgcg ccaccgctta aggacattga caccagtgcg
    11221 gccatgccgg tattggccgt cagcagcaac tattttctgg ttcggttaca tgtgcgctcc
    11281 ggggaacacc tgttcagtca gcagacttta atgcagtgga gggaggagcg tttccggatc
    11341 attcagcgac agtatggatt aacgatgagg gaggttccct gaaaaaaaat aacaaacatg
    11401 tcgaggctgg tcggcttttt ctgcgactgc cggaggatga ctcggagcct ttattgtgga
    11461 ggctgattgc gccagaggag acaggagccg gcagtgtggc agtcggagaa acggatccgc
    11521 agttagtcgg tttgctgtcc cgttatcccg cctgggtgct ggttccgtca ggcagaattg
    11581 ccttccatca ggtcacgctg ccacaccgca cgtggcgtca gcgtttgcag gtgctgccct
    11641 ttcttctgga agagcagttg gcgacagaca ttgagcagct gcacttcgcc attcttcatc
    11701 agtcgggtga caggtgtgat gtggcggtgg tcgaaaaagc ggttatgcat cgctggttag
    11761 cgtactgtgg acaacttggg gttcgggagg tgaggttgct gcctgacgta ctgatgctgc
    11821 cgttggcaac tgaaggatgg agtgccgtaa agcttgccgg gcagtggctg tttcgccggg
    11881 atcgttacgc cgggatgacg gtagagttat cgtggctgac acatttcttg tctcttactg
    11941 cgccgccggt gattgagtcc tactccgtgc cgccagtacc gtgcccgggt ccgaagacca
    12001 ctgagtggcg aaatcagccc ggacgcgatc tgctgcagtt ggtggccgaa ggtgacgggt
    12061 atgatggcgc agatctgcgg cagggagaat ttgcccgcca gggaacatgg tatgcacgat
    12121 ttcgcccatg gaggtatgtc gcaggggctc tgctggcctg tgtttttctg gccgggagca
    12181 acgcggggct ggcacattat cggctctggc agcaggctca attctggcgg tatgagagcg
    12241 tacgtgtcta tcagcagctg tttccgagtg aaaaggcggt gaaggatccc cgccggcaga
    12301 tgctgagaca tctgcaacag tcgacgggta acaatatacc ggaactcggg agcgtgatgc
    12361 gccagcttca gcaactgctg tcagaaacct caacgatacg cttacaggca ctcgcctggg
    12421 acagcagcag ccagacactg aaaattgatt tgcaggcggc ttcttttcag gcgctggaac
    12481 actttcagca gatcgccgga gaaaaatacc tcatccagcc tggtgagata cgacagcagc
    12541 cgcagggggt agaaagtcgt ctgatcctga gagtgaacaa tgaacgagct taaaaaacgt
    12601 ttgcagtatg ttagcccccg tgagcgcagg ctgcttatcg gatgtgttgc cttactggtt
    12661 gttgtttttg tctattacgt cctctggcag ccctggctga tacgagagga taagtggcgg
    12721 actgtagtta cccgtgaaaa aaacacagtg gagtggatga aacaacaggt acccaacata
    12781 aagagacggg aggcaagtat gcctgaacag ggggagccgt taggtttatc tgcggccgtg
    12841 acacgtactt cagccgcata cggtgtatcg gtcacccggt tgcagcctca aggggagcgt
    12901 ctggccgtca ccttagcccc ggttgaattt aatgcgctga tgcagtggct tacgcaactg
    12961 gagcgtcggc accgtattcg taccatggta tttgatgtgg ccgcgcaggg caacccaccc
    13021 ggacaggtaa cggttaatcg gctggttttg tcccaagatg acgtaaacaa ggacgcttcg
    13081 cttagtcgct gatgtcgtaa tttacagatt aatgcttgat gtaaaaattg gacttataaa
    13141 ggaggatgtg tgagtagttt gccggaattt gcggttgatt atcccttgtt gtggctcatc
    13201 tgcaccggag ggctgggggg cgttaccggc agttttctta acgtcgtgat tcatcgtctg
    13261 ccgataatgt tggagaggcg gtggcggcgt gaagcgctga cgctattggc acattctgtg
    13321 cctgagtccg ggccggcctt taacctgatg ataccgcagt ctcattgtcc ctgctgtttt
    13381 catccgctcg gcatcaggga taacattccc ctgttaagct acctatttct gaaggggaaa
    13441 tcacgctgtt gtggcgagcg tattcccgtt tactacccgc tagtcgaaat aacgaactca
    13501 gtgttattca ttctggccgc gagcaggttt ccaccagggc tgacattagc ggctgcctgg
    13561 ctttttattt ccatgctgtt ggttctggcg gttattgact gccatacggc gctgctgccg
    13621 gatgtgctga cgttgccatt gctctggctt ggcctgttat tcagtttaca gagaggcgtt
    13681 gtcaccttag aggaggcggt agtgggggcg gtatctgggt atctctgtct gtggggactg
    13741 tactggcttt ttcgtttcgc taccgggagg gaagggttag gttacggtga ttttaagcta
    13801 gctgcggcat tgggagcatg gatgggttgg caggcattac cctcaatact tttgtttgcc
    13861 tcagtcagtg ggttaatcgt aacagtactg ttgcgtattg taacagctac ggactttacc
    13921 cgcccgctgc cttttgggcc gtggctcgct ttgagcggag tgtgccattt tttgttgatg
    13981 tagcgttcgg ttaccggggc tagtaataaa aacggctcag cggataaaag aactgttgta
    14041 aaccggtatc aggttaaacg atacctagta aaaaatgatg ggtaatattt tgaaaaaact
    14101 gaattgtatc gcgtcattac tagtcttagt gactatctct ggttgtcatc agtcaccctc
    14161 catccataag caggccactg ttcctccgag cgaacaactg gaacaaatgg cgtcgattgt
    14221 ctctgcgaca cggtatctga aaatgagatg taaccgtagt gatttaccag atgaacagtc
    14281 gatcctaaat gtcgctaacc ggatcgctat cgggaaaggg tggcagtcat taactcagga
    14341 ggatatacgt aaacacagcg atgatatcta tgtgcgtctg actagggaca gtacgcctga
    14401 gtatataaaa tgcagagaat tcaatcggcg gctggtccca tttatcggtg aattattggc
    14461 aaggggtcgt ggataatatg aaccgccccg gaaatcctgg agactaaact ccctgagaaa
    14521 gaggtaaaca gcatgaataa aaatgggtct tcccttgtag tggtggctga aggcatgatg
    14581 attgtagtgg cacactgaat ttggccacct gaacagaggt gatatgctca cctcagaaca
    14641 acacaggtgc tccaatgaaa aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc
    14701 aactggttgt tgaccagaac tacacggtgg cagatgccgc caaagctatg gatatcggcc
    14761 tttccacaat gacaagatgg gtcaaacaac tgcgtgatga gcgtcagggc aaaacaccaa
    14821 aagcctctcc gataacacca gaacaaatcg aaatacgtga gctgaggaaa aagctacaac
    14881 gcattgaaat ggagaatgaa atattaaaaa aggctacaac cgcgctcttg atgtcagact
    14941 ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg tcacactctg
    15001 ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc ctgaaaaacc
    15061 agacggcaga cgggctgtat tacgcagtca ggtacttgag ttgcataaca tcagccatgg
    15121 ttctgccggg gcaagaagca tcgccacaat ggcaaccctg agaggcttca gaatggggcg
    15181 ctggcttgcc ggcaggctca tgaaagaact gggactggtc agttgccagc agcctgcgca
    15241 ccgttataaa cgaggtggtc gtgaacatgt cactatcccg aatcaccttg ggcggcagtt
    15301 cgcagtgaca gagccaaatc aggtatggtg cggcgacgtg acgtacatct ggacggggaa
    15361 acgttgggca taccttgccg ttgttctcga cctgtttgca aggaaaccgg taggttgggc
    15421 aatgtcgttc tctccggaca gcagactgac catcaaagcg ctgaaaatgg cctgggaaat
    15481 ccgcagtaaa ccagccgggg taatgttcca cagcgatcag ggcagccact atacaagcag
    15541 gcagttccgg cagttactgt ggcgttacca gatcaaacag agtctgagtc gacgaggaaa
    15601 ttgctgggat aacagcccga tggagcgctt cttcaggagt ctgaaaaacg agtggatacc
    15661 ggtgacgggt tacatgaact tcagcgatgc tgcccatgaa ataacggact atatcgttgg
    15721 gtattacaac gcgctcaggc cgcacgaata taacggtggg ttgccaccaa atgaatcgga
    15781 aaaccgatac tggaaaaact ctaaagcggt ggccagtttt tgttgaccac tacatcccgt
    15841 cagccgattt acgggccgta agtaacgaag ttggatgcaa atgtcagatc gtgtgcgcct
    15901 gttatggctc ggctggtaag agagccttat aggcgcattt gaaaaaactc cagctatacc
    15961 ggaagagatt tattattaac aggagaggtt tgcttttctg tgtaggttta gattttgtgt
    16021 ttgcagggta aacaaaactg attaactcgt catttcggta actgaaatag tttgctgtat
    16081 tctgtttaca atctctttaa taaggcattt gtcacgtggc tattcatatg aaaatcatac
    16141 gttatcttac aaatcaatca tctgagtgtt ataatataac ttagctgtga tatgtgtaag
    16201 aatgtttagg caatatatta ttatcgctaa tgttgttttg gttgggagag attctccttg
    16261 cggtagcttt ctgtttttag atgcttcttg cttaaaagga tataatttct gttcttttat
    16321 atagggttct ttacatttac tgagttttct tatttattta aacattatga aatctaatgc
    16381 ttttgacgtc atggggaagg ttgcatggct atgggcctgt tctcctctgc ataaaaaatg
    16441 gccattatct gtttttgcta ttaatgtcat acctgctatt cagacaaatc aatttgctct
    16501 cttaataaag gatgagcttc ctgtagcttt ttgtagctgg gccagtttag atctggaatg
    16561 tgaggtaaaa tatataaatg atgtaacatc tttgtatgca aaagactgga tgtcgggaga
    16621 aagaaagtgg tttattgact ggatagctcc atttggacac aacatggagt tatataaata
    16681 catgaggaaa aagtatcctt atgagttatt cagagcaata aggttagatg agagcagtaa
    16741 aacaggaaaa atagctgaat ttcatggagg ggggatagat aaaaaactgg catcaaaaat
    16801 attcaggcaa taccatcatg aacttatgtc tgaagtaaaa aacagacaag attttaattt
    16861 taatattgag aaagaaaact aatatgacag taaataaaat aaagaacatt ttcaataatg
    16921 cgacattgac tacaaaatca gcatttaata cagcatcatc aagcgtacgt tccgctggaa
    16981 aaaaactcat attattaata cctgataatt atgaagctca gggcgtgggt attaatgagt
    17041 tggtcaaagc tgctgatgag cttggaatag aaatacaccg tactgaacga gatgatacag
    17101 cgattgcaaa ccagtttttt ggtgcagcag aaaaagttgt aggattaact gaacgtggtg
    17161 ttgcaatatt cgcaccacaa cttgacaaac ttctgcagaa gtatcagaaa gttgggagta
    17221 aaataggagg aaccgctgaa aatgtaggta ataatctggg aaaagccgga acagttctct
    17281 cagcactaca gaattttacg gggattgctt tatcaggcat ggctcttgat gaattgctga
    17341 gaaaacaacg ggaaggagag gatataagtc agaatgatat tgccaaaagt agtattgaac
    17401 ttattaatca gcttgtagat acagtatcaa gtataaacag taccgttgat tcattttctg
    17461 agcagcttaa ccagcttggc tcatttttat ccagtaaacc tcgattaagt tctgttggtg
    17521 ggaaattaca aaatttacca gacctgggcc ccctggggga tgggctggat gttgtctccg
    17581 gaattctttc tgctgtatca gcaagcttta ttctgggaaa cagtgacgca catacaggaa
    17641 caaaagctgc agcgggtatc gaactgacaa ctcaggttct tggaaatgtt ggtaaagctg
    17701 tttcgcaata tattctggct cagagaatgg cacaggggtt atcgacaaca gctgcaagtg
    17761 cgggtctgat cacatcggct gttatgctgg ctatcagtcc tctttctttc ctggctgctg
    17821 cagataaatt tgagcgagct aagcagcttg aatcatattc tgaacgattt aaaaaattga
    17881 attatgaagg ggatgcttta ctcgcagcct ttcataaaga aaccggagct atagatgcag
    17941 ccctgacaac aataaatact gtcctgagtt ctgtatctgc gggagttagt gcagcctcca
    18001 gtgcatccct cataggggcc ccgataagca tgctggtgag tgcattaacc ggtacgatat
    18061 ctggcattct ggaagcatca aaacaggcta tgtttgagca cgttgcagag aaattcgctg
    18121 ctcggatcaa tgaatgggaa aaggagcatg gcaaaaatta ttttgagaat ggatatgacg
    18181 caagacatgc tgcgttttta gaagactctc tgtctttgct tgctgatttt tctcgtcagc
    18241 atgcagtaga aagagcagtc gcaataaccc agcaacattg ggatgagaag atcggtgaac
    18301 ttgcaggcat aacccgtaat gctgatcgca gtcagagtgg taaggcatat attaattatc
    18361 tggaaaatgg agggctttta gaggctcaac cgaaggagtt tacacaacaa gtgtttgatc
    18421 ctcaaaaagg gaccatagac ctttcaacag gtaatgtatc aagtgttttg acatttataa
    18481 caccaacatt taccccagga gaagaagtta gagaaagaaa acagagtggt aaatatgaat
    18541 atatgacatc tcttattgta aatggtaagg atacatggtc tgtaaaaggc ataaaaaatc
    18601 ataaaggtgt atatgattat tcaaaattga ttcagtttgt tgaaaagaat aacaaacact
    18661 atcaggcgag aataatttct gagctcggag ataaagacga tgtggtttat tctggagcag
    18721 gctcatcaga agtatttgct ggtgaaggtt atgataccgt atcttataat aagacggatg
    18781 ttggtaaact aacaattgat gcaacaggag catcaaaacc tggtgagtat atagtttcaa
    18841 aaaatatgta tggtgacgtg aaggtattgc aggaagtcgt taaggaacag gaggtgtcag
    18901 tagggaagcg aacagagaaa atacaatatc gtgattttga attcagaacc ggtggaattc
    18961 cttatgatgt aatagataat cttcattctg ttgaagagct cattggcgga aaacatgatg
    19021 atgaattcaa aggcggtaag tttaatgata tattccatgg cgcagatggg aacgattata
    19081 tcgaaggtaa ttatggtaat gatcgactat acggcgatga tggggatgat tatatatccg
    19141 gaggacaggg agacgaccag ttatttggtg gtagtggaaa cgataaattg agtggagggg
    19201 atggtaataa ttatctgaca ggaggaagcg gtaatgatga gcttcaggca cacggagctt
    19261 ataatattct gtcaggtggt actggtgatg ataaacttta tggtggtggt ggtattgatc
    19321 ttctggatgg aggggaaggt aatgactatc tgaatggtgg ttttggtaat gatatttatg
    19381 tttatgggca aaactatggt catcatacaa ttgcagatga aggaggtaaa ggagatcgtt
    19441 tgcacttatc tgatattagc tttgatgata tcgcatttaa gagagttgga aatgatctta
    19501 tcatgaataa agccattaat ggtgtacttt catttaatga gtcaaatgat gtcaatggga
    19561 taacatttaa aaactggttt gcgaaagatg cctcaggagc agataatcat cttgttgagg
    19621 ttataacaga taaagatggt cgagagataa aagttgataa gatacctcat aataataatg
    19681 aacggtcagg ttatataaaa gccagtaata tagcatctga aaaaaacatg gttaatatca
    19741 ccagtgttgc caatgatatt aataagatta tttcttcagt ttcagggttc gattcaggtg
    19801 atgaacgatt agcatcttta tataatttat ccttacatca aaacaacaca cactcaacaa
    19861 ctttaacgac aactgtctga gactaaaaga ggaggtgatg gccattccag gaggaatggt
    19921 catcgttata tgatgagtaa atgtagttct cataatagtc tgtatgcact gatattgctt
    19981 gcacaatatc ataatataac tgtcaatgct gaaactataa ggcatcagta taatacccac
    20041 acacaagatt ttggggtgac tgaatggtta ctggcagcga aatctattgg cttaaaagca
    20101 aaatatgtag aaaaacattt ttccagattg tcaataattt ctttacctgc gttgatatgg
    20161 cgggatgacg gtaagcatta tatattgtct cgtattacta aagattcatc acgctatctt
    20221 gtttatgatc cagaacaaca tcagtcacta acttttagtc gggatgagtt tgaaaaactg
    20281 tatcagggaa aagtcattct ggttacgtca agagcaacag tagtcggaga gttagctaaa
    20341 tttgattttt cttggtttat cccctctgtt gtgaaataca ggaggatttt acttgaggtg
    20401 ttaactgttt ctgcttttat tcagtttctt gcgttaataa cacctctttt ttttcaggtt
    20461 gtaatggata aggttttagt tcaccggggg ttttcaacgt taaatattat cacaatagca
    20521 tttattatag tgatactttt tgaagtgata ttaaccggag ccagaactta tattttctct
    20581 catactacaa gtcgtattga cgtcgaactg ggtgctaagt tattcagaca tttgcttgca
    20641 ttgcctgttt catattttga aaatcgcagg gtcggagaga ccgttgccag agtaagggaa
    20701 ctggagcaaa ttcgtaattt tttaaccgga caagcgttga catcagttct tgatctattt
    20761 ttttctgtaa tatttttttg tgtcatgtgg tattacagcc ctcaattaac actggttata
    20821 ttattgtcac taccttgtta tgttatatgg tcattgttta tatcaccctt attacgtcga
    20881 cgtcttgatg ataagtttct caggaatgca gaaaatcaag cttttcttgt cgaaacggta
    20941 acagcaataa atacaatcaa atccatggca gtatcaccac agatgattgc tacatgggat
    21001 aaacaactgg ccgggtatgt tgcttccagt ttcagggtaa atctggttgc aatgacaggg
    21061 cagcagggga tacagctgat acagaaaagt gtaatggtaa ttagtctatg gatgggagca
    21121 catcttgtca tatcgggaga gataagcatc gggcagttaa tcgcgtttaa tatgcttgca
    21181 ggtcaggtca ttgctcctgt tatcagactg gctcatcttt ggcaggattt tcagcaggtt
    21241 ggtatttctg ttgagcgcct tggtgatgta ttaaatacac cggtagagaa aaagtcaggc
    21301 agaaatatac tgccggaaat tcagggggat atcgaattta aaaatgtcag atttcggtat
    21361 tcttctgacg gtaatgttat tttgaataat attaatttat acatatcaaa gggggatgtt
    21421 atcggtatag ttggccgttc tggttcagga aaaagtacat taacaaaact gcttcagcgc
    21481 ttttatatac cagagaccgg acagatttta attgatgggc atgatttatc acttgcagat
    21541 ccagaatggt tacgacgcca gattggtgtt gtattgcagg aaaatatact attaaatcgt
    21601 agtattatcg ataatattac attagcttct ccggctgtat ctatggaaca ggctattgag
    21661 gcagccagac ttgcaggtgc ccatgatttt attagagaac taaaagaagg gtacaatact
    21721 attgttggag aacagggcgt tggtctttca ggagggcagc gccaacggat cgcaatagcc
    21781 cgggctcttg ttacaaatcc tcgaattctt atttttgatg aagcaaccag t
//
LOCUS       DAZFKY010000072        21467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFKY010000072 DAZFKY010000000
VERSION     DAZFKY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            428..708
                     /gene="drpB"
                     /locus_tag="ACHKCL_002375"
                     /pseudo
     CDS             428..708
                     /gene="drpB"
                     /locus_tag="ACHKCL_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
     gene            748..1443
                     /gene="yedJ"
                     /locus_tag="ACHKCL_002376"
     CDS             748..1443
                     /gene="yedJ"
                     /locus_tag="ACHKCL_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5763334.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1510..2928
                     /gene="dcm"
                     /locus_tag="ACHKCL_002377"
     CDS             1510..2928
                     /gene="dcm"
                     /locus_tag="ACHKCL_002377"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5763335.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2909..3379
                     /gene="vsr"
                     /locus_tag="ACHKCL_002378"
     CDS             2909..3379
                     /gene="vsr"
                     /locus_tag="ACHKCL_002378"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5763336.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3368..4288)
                     /gene="yedA"
                     /locus_tag="ACHKCL_002379"
     CDS             complement(3368..4288)
                     /gene="yedA"
                     /locus_tag="ACHKCL_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5763337.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4461..5378
                     /gene="yedI"
                     /locus_tag="ACHKCL_002380"
     CDS             4461..5378
                     /gene="yedI"
                     /locus_tag="ACHKCL_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5763338.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5457..5639
                     /gene="yodC"
                     /locus_tag="ACHKCL_002381"
     CDS             5457..5639
                     /gene="yodC"
                     /locus_tag="ACHKCL_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5763339.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5810..7504
                     /gene="dgcQ"
                     /locus_tag="ACHKCL_002382"
     CDS             5810..7504
                     /gene="dgcQ"
                     /locus_tag="ACHKCL_002382"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5763340.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7501..8316)
                     /gene="yedP"
                     /locus_tag="ACHKCL_002383"
     CDS             complement(7501..8316)
                     /gene="yedP"
                     /locus_tag="ACHKCL_002383"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5763341.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8614..8841)
                     /gene="yodD"
                     /locus_tag="ACHKCL_002384"
     CDS             complement(8614..8841)
                     /gene="yodD"
                     /locus_tag="ACHKCL_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5763342.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9004..9192
                     /gene="dsrB"
                     /locus_tag="ACHKCL_002385"
     CDS             9004..9192
                     /gene="dsrB"
                     /locus_tag="ACHKCL_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5763343.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9236..9859)
                     /gene="rcsA"
                     /locus_tag="ACHKCL_002386"
     CDS             complement(9236..9859)
                     /gene="rcsA"
                     /locus_tag="ACHKCL_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5763344.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10149..10934)
                     /gene="fliR"
                     /locus_tag="ACHKCL_002387"
     CDS             complement(10149..10934)
                     /gene="fliR"
                     /locus_tag="ACHKCL_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5763345.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10942..11211)
                     /gene="fliQ"
                     /locus_tag="ACHKCL_002388"
     CDS             complement(10942..11211)
                     /gene="fliQ"
                     /locus_tag="ACHKCL_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5763346.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11221..11958)
                     /gene="fliP"
                     /locus_tag="ACHKCL_002389"
     CDS             complement(11221..11958)
                     /gene="fliP"
                     /locus_tag="ACHKCL_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5763347.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11958..12323)
                     /gene="fliO"
                     /locus_tag="ACHKCL_002390"
     CDS             complement(11958..12323)
                     /gene="fliO"
                     /locus_tag="ACHKCL_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5763348.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12326..12739)
                     /gene="fliN"
                     /locus_tag="ACHKCL_002391"
     CDS             complement(12326..12739)
                     /gene="fliN"
                     /locus_tag="ACHKCL_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5763349.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12736..13740)
                     /gene="fliM"
                     /locus_tag="ACHKCL_002392"
     CDS             complement(12736..13740)
                     /gene="fliM"
                     /locus_tag="ACHKCL_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5763350.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13745..14209)
                     /gene="fliL"
                     /locus_tag="ACHKCL_002393"
     CDS             complement(13745..14209)
                     /gene="fliL"
                     /locus_tag="ACHKCL_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5763351.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14314..15441)
                     /gene="fliK"
                     /locus_tag="ACHKCL_002394"
     CDS             complement(14314..15441)
                     /gene="fliK"
                     /locus_tag="ACHKCL_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5763352.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15438..15881)
                     /gene="fliJ"
                     /locus_tag="ACHKCL_002395"
     CDS             complement(15438..15881)
                     /gene="fliJ"
                     /locus_tag="ACHKCL_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5763353.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15900..17273)
                     /gene="fliI"
                     /locus_tag="ACHKCL_002396"
     CDS             complement(15900..17273)
                     /gene="fliI"
                     /locus_tag="ACHKCL_002396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5763354.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17335..18021)
                     /gene="fliH"
                     /locus_tag="ACHKCL_002397"
     CDS             complement(17335..18021)
                     /gene="fliH"
                     /locus_tag="ACHKCL_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5763355.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18014..19009)
                     /gene="fliG"
                     /locus_tag="ACHKCL_002398"
     CDS             complement(18014..19009)
                     /gene="fliG"
                     /locus_tag="ACHKCL_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5763356.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19002..20660)
                     /gene="fliF"
                     /locus_tag="ACHKCL_002399"
     CDS             complement(19002..20660)
                     /gene="fliF"
                     /locus_tag="ACHKCL_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5763357.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20875..21189
                     /gene="fliE"
                     /locus_tag="ACHKCL_002400"
     CDS             20875..21189
                     /gene="fliE"
                     /locus_tag="ACHKCL_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5763358.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(21214..21354)
                     /locus_tag="ACHKCL_002401"
                     /pseudo
     CDS             complement(21214..21354)
                     /locus_tag="ACHKCL_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         5092 a         5513 c         5688 g         5174 t
ORIGIN      
        1 atgttatgag ttgatcggaa agatgggggg tatcaatgat tgttgcgcta aacggctggt
       61 gtgatcacgc gcacattaat gaaggcataa aaaaaagccg gcatcatgcc ggcccaaaaa
      121 atataaacaa gacgataaaa ggctaataac ggaagcatca tgacacagaa taataaaaag
      181 gtataattgt aatcagtgct aatatattga ttgaatgaat taataaagcc gatctcaaag
      241 ataaataaaa tcgctcaaaa cgatttttcc tgtattcact gccgttgcgc aaaaattatc
      301 tatttgttca aaaaatgagt gagtcttgac tggctcatcc aatgtggaaa aatgtgactt
      361 ttatcacgta atagtactaa gtctgaattt tccgggttat ctcaaaatgg aatacggttc
      421 gacaaagatg gaagagagac tctctcgcag ccctggcggc aaactggctt tgtgggcttt
      481 ttatacatgg tgtggctatt tcgtctgggc aatggcgcgt tacatctggg tgatgagccg
      541 gatccccgat gcccccgtgt cgggatttga aagcgattta ggctctaccg cgggaaagtg
      601 gttagggcac tggtcggatt tttatttatg gctctggtcg gggcattgtt aggcagcatt
      661 gcctggtaca cacgacctcg tcccgcacgt agcagacgct atgaataaaa actgctaagg
      721 tggcgactct ttggcaacgg agcgcgtatg gacttacaac actggcaggc acagttcgaa
      781 aactggttga aaaaccatca tcagcaccag gacgcggccc atgacgtgtg tcattttcgc
      841 cgcgtctggg ctacggcaca gaagctggcg gcagatgatg acgtcgatat gctggtgatt
      901 ttaaccgcct gttatttcca cgatattgtg agcctggcga aaaatcatcc gcaacggcag
      961 agttcttcaa tcctggcggc agaagagacg cgtcgcctgc tccgtgaaga gttcgtgcaa
     1021 tttccggcgg agaaaatcga ggccgtttgt catgccattg ccgctcacag tttcagcgcg
     1081 caaatcgccc ccttaacaac ggaagctaaa atcgtccagg atgcggatcg gctggaagcc
     1141 ctgggggcga ttggcctggc gcgcgtgttt gccgtttcag gggcattggg cgtggcgctg
     1201 tttgatgccg aagatccgct cgcacagcat cgcccgcttg atgataaacg ctacgcgctg
     1261 gatcatttcc agactaagtt gttgaagcta ccgcaaatca tgcaaaccgt aagaggcaag
     1321 cagctggcgc agcacaacgc gcatttttta gtcgagttta tggcgaagct cagtgccgaa
     1381 ctggcggggg agaatgaagg tgtcgatcac aaggtgatag atgcgttttc acccgcgggc
     1441 tgagcgcgtg gccctaaatg gctgtaatta tgttaacctg tcgaccatct cagatggccg
     1501 gttaaatcta tgcaggaaaa tatatcagta accgattcat acagcaccgg gaatgccgca
     1561 caggcaatgc tggagaaact gctgcaaatt tatgatgtta aaacgctggt ggctcagctt
     1621 aatggtgtgg gtgagaatca ctggagcgcg gcaattttaa aacgtgcgct ggcgaatgac
     1681 tcggcatggc accgtttaag tgagaaagag ttcgcccatc tgcaaacgtt gttacccaaa
     1741 ccaccggcac atcatccgca ttatgcgttt cgctttatcg atctatttgc cggaattggc
     1801 ggcatccgtc gcggttttga atcgattggc ggacagtgcg tgtttaccag cgaatggaac
     1861 aaacatgcgg tacgcactta taaagccaat cattattgcg acccggcgac gcatcatttt
     1921 aatgaagata tccgcgacat caccctcagc cataaagaag gcgtgagtga tgaggctgcg
     1981 gcggaacata ttcgtcaaca cattcctgaa cacgatgttt tactggccgg tttcccttgt
     2041 cagccatttt cgctggctgg cgtatcgaaa aagaactcgc tcgggcgggc gcacggtttt
     2101 gcctgcgata ctcagggcac gctgttcttt gatgtggtgc gcattatcga cgcgcgtcgt
     2161 ccggcgatgt ttgtgctcga aaacgtcaaa aacctgaaaa gccacgacca gggtaaaacg
     2221 ttccgcatca tcatgcagac gctggacgaa ctgggctatg acgtggctga tgcagaagat
     2281 aacgggccgg acgatccgaa aatcatcgat ggcaaacatt ttctgccgca gcaccgtgaa
     2341 cgcatcgtgc tggtgggttt tcgtcgcgat cttaatctga aagccgattt tactctgcgt
     2401 gatatcagcg aatgtttccc tgcacagcga gtgacgctgg cgcagctgtt ggacccgatg
     2461 gtcgaggcga aatatatcct gacgccggtg ctgtggaagt acctctatcg atatgcgaaa
     2521 aaacatcagg cgcgcggaaa cggcttcggt tatggaatgg tttatccgaa caatccgcaa
     2581 agcgtcaccc gtacgctgtc tgcgcgttat tacaaagatg gcgcggaaat tttaatcgat
     2641 cgcggctggg atatggccac gggtgagaaa gactttgacg atccgctgaa tcagcaacat
     2701 cgtccacgtc ggttaacgcc tcgggaatgc gcgcgcttaa tgggttttga agcgccggga
     2761 gaagcgaaat tccgcattcc ggtttcggac actcaggcct atcgccagtt cggtaactcg
     2821 gtggtcgtgc cggtctttgc cgcggtggca aaactgcttg agccaaaaat caaacaggcg
     2881 gtggcgttgc gtcagcaaga ggcacaacat ggccgacgtt cacgataagg ccactcgcag
     2941 caaaaatatg cgcgcgattg ccacgcgtga tacggcgatt gagaagcgcc tcgccagtct
     3001 gttaaccggg cagggcctgg cgtttcgcgt tcaggacgcc agtctgcccg gacgcccgga
     3061 ttttgtcgtt gatgaatatc gctgcgtgat atttacccat ggctgcttct ggcatcatca
     3121 tcactgctat ctgtttaaag tgcctgcgac tcgaaccgag ttctggctgg agaagatagg
     3181 taaaaatgtt gagcgcgatc gccgcgatat cagtcgcttg caggaactcg gctggcgcgt
     3241 attgattgtc tgggagtgcg cgttacgtgg gcgcgagaag ctgaaggatg aagcgcttac
     3301 cgagcgtctg gaagagtgga tctgcggcga aggagccagc gcgcagatcg acacgcaggg
     3361 gatacattta ctcgcttgat gcgtcctgaa taactggagc aactacgggt tttgccggaa
     3421 agagatattt tcccaacgtg accagtacca ccgcgaagac aattacgccg agcgccagcc
     3481 attcaatctt cgacagtgtt tctccaccca gtcccgtacc cagcaagacc gcgaccaccg
     3541 ggttaacgta agcgtagctg gtggcgagag ccggactgac attacggatt aaatacatat
     3601 aagcgttgat ggcgataatc gaaccaaaca gcgccagata gccgaccgca aggaagcctg
     3661 aaagggaagg gagcgccgtc agtttttcac ccgcaatcat cgacgcgatc attaacacca
     3721 cgcctgccgc cagcatctca atcgcacccg ccatcatccc tacaggtaag gtaatgcgcg
     3781 agccataaac tgagccaaac gcccagctaa tcgagccgat taaaatcaga atcgcgcccc
     3841 acggattgcc gcttaaattt ccaccgctat tgagcatgat gattccggca agcccaatgg
     3901 caatacccac ccattccagt ttgcgcgttt taatgccaaa caggcggctg aagcacaggg
     3961 taaagagggg cacggttgca accactacgg cggcgatgcc ggaaggaaca ttttgatgtt
     4021 cggcaaccgt caccatgcca ttaccgacag ccagcaataa caggccaatc agcgcggcat
     4081 tgagcagcgg acgtagcggg gggagtttgt gtccgcgcag tagtaaaaat gccagcaata
     4141 aaataccggc tgccaggaat cgaacacccg ccatcattaa cggaggccag ctttccacgc
     4201 caatccgaat gacaaaatag gttgagcccc aaatgatata caacgcaaac agcgcgccaa
     4261 aaagcggtaa caactggcgg aaacgcataa tccctcacgg tggaaataaa aaggtggttc
     4321 atagtaaacg tgaaaatcat tctgctggcg agagatataa ttgcacttga ttgttaaaaa
     4381 aatgttgacc tgtgaagcaa gtatcagagc tgccgttttt gcttcatact tacacccttc
     4441 aacaataaaa atgagaggga atgcttttgg ccgggagtag tttactgacg ttgctcgatg
     4501 atatcgccac actgctggac gatatctccg tgatgggcaa actggcggcg aagaaaaccg
     4561 ccggtgtatt aggggatgac ttatcgctca atgcgcaaca agtttcaggc gtgcgggcca
     4621 accgggaact tcccgtggtc tggggcgtgg cgaaaggatc gctgattaat aaagtgattc
     4681 tggtgccact ggcgctgatc atcagtgcgt ttatcccgtg ggcgattacg ccattgttga
     4741 tgatcggtgg cgcgtttctc tgctttgaag gggtagagaa agtgctgcat atgctggagg
     4801 cgcgtaaaca taaagaagat ccggcgcaga gccagcagcg tctggagaag ctggcggcgc
     4861 aggatccgct gaagtttgaa aaggacaaaa taaaaggggc gattcgtacc gattttatat
     4921 tgtctgcgga aatcgtcgcc atcacgctgg ggattgtggc cgaagcgccg ttgcttaatc
     4981 aggtgctggt gctttcaggc atcgcgctgg tagtgaccgt gggcgtctac ggtctggtcg
     5041 gggttatcgt taagattgat gacctggggt attggctggc ggaaaaatcc agcgcgctga
     5101 tgcaggcatt aggtaaagga ttattgatta tcgcgccctg gctgatgaaa gcgttatcga
     5161 ttgtcggcac gctggcgatg ttcctcgtcg gcggcgggat tgtggtacat ggtattgcgc
     5221 cgctgcatca cgccattgaa catttcgccg ggcagcaaag tgcagtggtg gcgatgatat
     5281 taccgactgt tttaaatctg attcttggat ttatcatcgg cggcatcgtg gtgctgggag
     5341 tgaaagccgt agcgaaaatg cgcggtcagg tacattaata ctcgcacagg ctattatgca
     5401 aaattcgcca tcttcaacta aggtgaacaa gtttcactcg aaaaaaggag gcgagtatga
     5461 gctttatggt tagtgaggaa gttacggtta aagagggcgg cccgcggatg attgtcactg
     5521 gatactccag cggtatggtt gagtgtcgtt ggtatgacgg ttacggggtc aagcgggaag
     5581 cttttcatga aaccgagctt gttccagggg aggggagtcg ttctgcggaa gaagtttgaa
     5641 agggtatcat taagaccagg ccggatcaca tccggcctgg tgtgccaatg cctgcaatga
     5701 atgatagtcg cgtcttatca tgccaccatt ataaaattat cgctgttttg cggtacgcta
     5761 tcgccaggtt atgccagaat cataaaaaag caggtgggga gtcgtcaggg tgcagcacga
     5821 gacaaaaatg gaaaaccaga gctggttgaa aaaactcgca cgccgcctag ggcctggtca
     5881 tatcgttaat ctctgcttta tcgtggtatt gcttttttcc accttgctga cctggcgtga
     5941 agtagtggtg ctggaagatg cctatatctc cagccagcgt aatcatctgg aaaacgtagc
     6001 caacgcgctc gataagcatt tgcagtataa cgtcgacaaa ctgatctttt tgcgtaatgg
     6061 catgcgcgaa gctctcgtag cgccactgga tttcacctct ctgcgtgatg ctgtaaccga
     6121 gttcgaacag catcgcgacg agcacgcctg gcaaatcgaa ctcaaccgac gacgcaccct
     6181 gtcagtcaat ggcgtatcgg atgcattagt cagcgagggg aatctcctgt ctcgcgaaaa
     6241 tgaaagcctc gacaatgaaa ttaccgctgc actggaagtt ggttacttgc tgcgactggc
     6301 acacaactcc tcgtcgatgg tagaacaggc gatgtatgtc tcgcgtgccg gattttacgt
     6361 ttcgacgcag ccgaccttgt ttacgcgcaa tgtcccgacg cgttattacg gctatgtcac
     6421 ccaaccctgg tttatcggcc attcacaacg agaaaatcgt caccgcgcgg tacgctggtt
     6481 cacttcgcaa ccggaacacg ccagcaatac tgaaccgcag gttaccgtca gtgttccggt
     6541 agacagtaat aactactggt atggcgtgct ggggatgagt attcccgtgc gtactatgca
     6601 gcaattttta agaaacgcca tcgataaaaa cctcgatggt gagtatcagc tctatgacag
     6661 taagctgaga tttttgacct cttccaatcc tgaccatcca acagggaata tttttgatcc
     6721 tcgtgaactg gccttgctgg cgcaggcaat ggaacatgac acgcggggcg gcattcgtat
     6781 ggacagtcgc tatgttagct gggaacgtct ggaccatttc gacggtgtgc tggtgcgcgt
     6841 ccatacgtta agcgaaggcg tgcgcggcga tttcggcagt atcagcattg cattaaccct
     6901 gctgtgggcg ctctttacca ccatgttact catctcctgg tatgtgattc gccggatggt
     6961 cagcaacatg tatgttctgc aaagctcgtt gcagtggcag gcgtggcacg acaccttaac
     7021 gcgtttatat aaccgtggcg cactgttcga aaaagcccgt ccgctcgcga aattgtgtca
     7081 gacgcaccaa catccttttt ctgtcattca ggtcgatctt gaccatttta aagcgattaa
     7141 tgaccgcttt ggtcatcagg cgggcgaccg tgttctttct catgctgccg gattaattag
     7201 cagttccttg cgtgcgcagg acgttgccgg gcgggtcggt ggtgaggagt tttgtgtgat
     7261 tctgccaggc gcgagtctga cggaggctgc ggaagtcgca gaacgtattc gcctgaagtt
     7321 aaatgaaaaa gagatgttga ttgctaagag tacgacgata cgcatcagtg cctcgttggg
     7381 ggtaagtagc agcgaggaaa ccggtgatta tgattttgaa caactccagt cactggccga
     7441 ccgtcggctt tatctcgcta aacaggctgg gcgcaatcgg gtattcgcga gcgataacgc
     7501 ttaacgggcg gagaaaaaat ggtccagccc ttcacgccat ccttccggtc cttcacgctg
     7561 cgttcgccag acgcgggccg gatcctcatc atgcagatgc accccttcac ggtttagccc
     7621 tttcacaatc accgcgtaat ccattacctc cagtaagggc gcatcgtttg gcccatcgcc
     7681 caggccaagt gtggttgggc gtttgcctga caattgttga taggtcgcga taatccagtt
     7741 ggcagcctga tcttttccgg cagaggcatc caggacgtgc cagaagcgcg caccttgcat
     7801 aaactgtaag cccagttcgt tcagacgagc ggtaaattgt gccatacgct cgtcactgtc
     7861 gcgccagatt agcgttaccg acgcctcatg aagctgcgtc agcgccgcct ggctacggct
     7921 taatcccgtc cattcggcga tggttgcatc gtcgacatca tcaaaagtcg tgaatttaaa
     7981 atgttctttc tcgcgtagcg tatttaaaac ctggctgatt tcgccatggc taatacctga
     8041 gatgatgcgt ggaaaaccgt ctatctcctg ccattgttca gcaagctgga tcactgcgcc
     8101 attctctgca atcagcggta aaccttgtag ccccaacatt ttttgcaagt acagcatttc
     8161 cgctgatgtt ttactgctac agagaatgac gggaacattt gcttcgcgta aacggctgag
     8221 ccagggggct gccggttgcc agtcataact atgactgtcc agcagggtgc catcaagatc
     8281 gctaaaaacc agtagtggtt gttgaattga aaacatagtc gcgcagtact ccccttacca
     8341 ggatgattct caaaacagca caaattatca aagatgattt tttcgggagc gatcccgctg
     8401 acgccagagc caccaagggg tgacgtgcgt cacatttcta ttcataagta gcgttaatca
     8461 ttcatatagc gaatattttc ttgtcagcga aaaaaattgc ggataaggtg atgaacacat
     8521 cagatttcct ggtgtaacga attttttaag tgcttcttgc ttaagcaagt ttcatcccga
     8581 ccccctcagg gtcgggattt ttttattgtg cattcaacga ttcacttcag agacgctgaa
     8641 gtcatgaata tccagttcga aggcatccgc caattcacgc catgtatgat attcacgtcc
     8701 atcgacgcta acgtgttcag aatcgttctg gctgcgatga acttcgcttt cgctaatttc
     8761 attgatcgcc gccagcaggg catcgacatc gacgctgacc tcacgttttg cggtatcgct
     8821 gtactctttt gcggttttca tcatctttcc tcgcaaccgt tactctgttg ttgccgccag
     8881 tcgttaagtc cgcctggcat ctcttaagta tagaccgtcg agaaaatcag cactcgcgcg
     8941 cgcctggcgc tgccagaaaa acgaaattgt tctacactgg cacaaagcca caggaggaaa
     9001 acgatgaagg tgaatgatcg ggtaacagtc aaaacggatg gcggtccgcg tcgtcctggc
     9061 gtggtactgg cagttgagga gtttagtgaa ggcacaatgt acctggtttc gctggaagac
     9121 tacccgctcg gcatctggtt ctttaatgaa gcagggcatc aggacggtat ctttgtggag
     9181 aaagcagagt aatctaattc agcctgactg gtgggaaacc accagtcaga atgtgttagc
     9241 gcatgttgac aaaaatacca ttagtcacat tatccgtcag tcggacgaca tggtagataa
     9301 cctgtttatt atgcgttttg atcttacgtt taatattacc tttatgcgat gaaacggtct
     9361 tggctttgat attcatttgg tcggagattt gaatggttcc ctgacctgcc atccacattc
     9421 gcaacatact cgattcggtt cggctcaatg ataacgtcgg catatttaaa aacgaggtta
     9481 tcgttgtctc ttttttcaga atatcgccaa ggatatcgtc gagagattcc ggtttaatcg
     9541 atttagaact gatcaataaa ttttttctga ccaatagata ttcatcaaaa tgaacattgg
     9601 caattgccat aaaaacgata aataacgtat tgggatgttg attaatgatg agcttgatac
     9661 gctgactgtt agaagcatcg tggatgaaac agtcctcatt aataaacacc actgaagggc
     9721 gctgtgaatc acaagctatg gcaaggtcat caacggtttc aatgtcgttg atttctcttt
     9781 ttttaacccc tctactcaac agatacccgg ttaaacctag tcgggtgtaa ctacataaat
     9841 ccataataat cgttgacatg gcataccctc actcaatgcg taacgataat tccccttacc
     9901 tgaatatttc atcatgacta aacggaacaa catgggtcac ctaatgcgcc actctcgcga
     9961 tttttcaggc ggacttacta tcccgtaaag tgttgtataa tttgcctgga attgtcttaa
    10021 agtaaagtaa atgttgcgat atgtgagtga gcttaaaaca aatatttcgc tgcaggagta
    10081 tcctggaaga tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg
    10141 ttacggaatt atattaatgg caattcacta ataatatcag ccagcaaatt aaaaatttca
    10201 ctgaataaat gttcgcaaaa aggcgcaatt aacggcatta atgccgccat taaagagatg
    10261 ccgacagtca gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga
    10321 ttaagtaaac ctaatgccag attcagtgtc agcagcagag taatgagcgg taacgccagc
    10381 atcagcccgt taaggaaaat caaactccct gctttggtga gtgccagaaa cgcattgctg
    10441 ttcaacggtt cgccaccaat cggcagggtg tgaaaggtat cgaccagcag tgaaatcaac
    10501 cataaatgac cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct
    10561 aaaacgggca tattaagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt
    10621 tgcagaccga taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa
    10681 ccaagcgcaa tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa
    10741 acaggaacat cgttggcagg taatgatggg gcaatggcga acgtgatcat cattgccaga
    10801 cccagtttta cccgtttcgg tacgctgcgt tcgctcagaa tcggcgcggt ggagatcagc
    10861 gccagcacgc gcagtaacgg ccagaagtac aggcttaacc aggatagcca ttgttcgctt
    10921 gtcacctgca acatagtacg gctacccgat gatatacggc aggttagtga acaaggtgcg
    10981 gacgtaatcc agcaacagat tgagcatcca cggtccggca ataataatgg cgataaatac
    11041 ggcgatgatt ttcggaataa acgacagcgt catttcgtta atctgcgtgg cggcctgcaa
    11101 aatactgatg ataaggcccg tgaccaacgc taccaacaat agcggggcag ccagtgccag
    11161 cgcgactttc atcgcttcag tccccatcat catgaccgat tcaggtgtca ttttgcctct
    11221 ctagctgtaa aagctctgcg ccagcgaacc gaccagcaat tgccagccat ccaccagtac
    11281 aaacagcatc agtttaaagg gcagagcaat ggtggcgggg ggaaccatca tcatccccaa
    11341 tgccatcaac acgctggcta tcaccaggtc gataatcaaa aaagggatga aaatcgtgaa
    11401 gcctatctga aatgcggttt tcaactcgct ggtcacgtag gccgggagca aaatgcgcat
    11461 cggcacggct tcaggtccct gcaacgggcc ggtattcgcc agtctggcaa acaaccctaa
    11521 atctgcctca cgggtctgac gcagcataaa ctcacgcagc ggctgcgccc ctttttccag
    11581 cgcctcctgc attgatattt tctcttcgct gaatggctgg tacgcatcga cataaatttt
    11641 gtcgatcacc ggtgacataa taaaaaaggt caaaaacagt gccagcccca gcaatacctg
    11701 gttaggtggc gcggagggtg ttcccagcgc gttacgcaat aaaccaaaaa caatgatgat
    11761 gcgggtgaaa ctggtcatca tcagtaaaat tgccggaata aacgtcaacg aggtgatgaa
    11821 caccagcgtc tgcaccggga gcgaccagct ttgtccaccg ccaggcagcg gctggctggt
    11881 gatacccggc agttgcgcga aggcgagggg cgtaatcagc cagagaagga caggtgcgac
    11941 agacaataaa cgacgcatca ggatctcccg ctacgcttaa gcaaattttt catgaccgac
    12001 tgaaaatcgg tctgcggtat ctcttctgtt ggtgctgaag ggggaagttt atgcagcaga
    12061 ttgatttgcc ctgcggtaac gccgagcacc agccgtgcat cttccacatc gaccaccaca
    12121 acccgttcac gcgcgcccag cgaagcactg gcgctaattt tcagaccgtt aacgccagtg
    12181 cgtttcgggg caaatcccaa ccgttttacc agccaggcag cagcgaggat cagggcaata
    12241 atggcgatga gtgcgccgct cacctgcagc agtggcgcag cagaaaccgg cgcggaagat
    12301 tgcacagtag cgtggttatt catcactaac ggctcaggcg gcgcattcgc tcagacggag
    12361 taatgatatc ggtgatccgc acgccatatt tatcggcaac gaccaccact tcgccctggg
    12421 cgattaaata accgttgatc agaatatcca gtggttcgcc cgccagaccg tccagcgcca
    12481 cgacggaccc ttgcgtcaga cgcaacagct ctttgatggt cattcgtgtg cggcccagct
    12541 cgacggtcag cttgaccgga atatccataa tcaggtcgat atcctgcaac gttccgctga
    12601 catcaccacc gccaaattgc tggaacaccg cgtcggcagc gcttttgctg ctggttgatt
    12661 tttgttcgct caacgcttca gcccacagat cgtccattgc gccgttgttg tcatcggccg
    12721 gattattcat gtcactcatt tgggctgttc ctcgttcaga gaatttaaaa tcgggttaat
    12781 caaatgttct atccgtaacg catactgacc gttgagagtg ccgtactgac tggtcagcac
    12841 cgggacgccg tcaacatggg cgatgatgcg gtcgggtttt tctatcggca ggacgtcacc
    12901 gggtttcagt tttaaaatct gcgacaggcg tagcgagata tcggcaaagt tggcgaccag
    12961 ctccagctgt gaatgctgca cctggcgcac caggttatcg cgccagttct gatcttcatt
    13021 acgcgagttt tccagcggcg ggttaaccaa caattcccgt agcggctcga tcatgctgaa
    13081 tggcaggcag atattaaatt cgccggtcag gttgccaatc tccacatgga acggcgtgtt
    13141 aaccacaatg tcgttcggcg aggtggtgat attggtaaat ttcacctgca tttccgaacg
    13201 cacgtactca acttccagcg gattaatcgc cttccatgcg tcgctatagc cttcaagcgc
    13261 cagtttcaac atgcggttga tgacgcgctg ttcggtatgg gtaaactcgc gaccttccac
    13321 tttggtcggg aagcgtccat cgccgccaaa caggttatcc acggcgataa acaccagact
    13381 cggtgagaac accaccagcc cagtgccgcg cagcggtttt agatggataa ggttcaggtt
    13441 ggtcggcacc ggcaggttgc gggcaaattc atggtacggc tgaatgcgga tggccccgac
    13501 ggttatatcc gggctacgac gcagcaggtt gaacagcccc atacgaaaat ggcgggcaaa
    13561 gcgctcatta atgatttcca gcgcctgcaa acgttcgcgc acaacccgtc gttgggtatt
    13621 cggatcgtac ggacgaatgt cactttcgcc gctaacactg gctgtcggtt cgtctttgac
    13681 ttcgctgtca ccattcaaca gcgcatcaat ttcagcttga gaaagaatac tatcgcccat
    13741 gtcgttatcg cagaataaaa gcggtataca gcacgtcggt gacatcctgt ttcggttgcc
    13801 cggcaacaag cggggtggaa agtgtggttt taatctcggc aatcaggttt ttcttgcctt
    13861 cttctgtcgc cagtacggca gcatcctgac gcgaaaacag taacagcaaa cggctacgga
    13921 cttccggcaa atactcactc agccgcgagc gggtagcttc atctttcagg cgcagggtta
    13981 tgccgatata aagcacgcga tccgcatcgc ccagattgac cgtaaaggta tccagcgcat
    14041 agaagaccgg ggagggcacg acgcgttgct gcgctttgtc gtcagcagca acctgatgcg
    14101 aatgccagta gctgtaacct gcgctggcac aggccgcgag ggtaatgaat accagaatcg
    14161 ggatccaaag cgatcgcttg cttttcttgc ttatcgcgta atcagtcatg tgttgcggtc
    14221 ttcctgtgtc gctactgctt atcggctaat tatcctgcgt cttgagcgca acaaagcggg
    14281 gaaaagacgc ggattacggt gctacctctg acgttaggcg aaaatatcaa cgccgctgtt
    14341 gcctgttacg cgcccttgta aagagacggg aaccggaagc gtatcgtcgt cttcccccgc
    14401 cagaggttca tggtttactg tgcgttggct ttgctgttgt tgggaagcgg cctgctgctg
    14461 accactaaag ctttcgccac tgatgttgct ttgccctaac tgaatgccac tttcggccag
    14521 ctgcgtacgc agtaccggca gcgctgcttc cagggcggcg cgtacatgct gatgcgatga
    14581 aaccatctgg atttgcgcct ggttatcatc cactttgagg gagatttgca cttcacctaa
    14641 atcctgcggg tgcagacgca actctgcact ttgttgcccc tggcgggtga acagcgaaat
    14701 atgctggctt aatgattgtt gccattcgtg agaacccagc ggtgcactca aaacaggcgc
    14761 ggcgacggtg ggcagtggct gtgtctggtg tggagtgatt agcgggctgg cggcagcagt
    14821 caccggcgaa ggcgtgctga tgacttccgc tttactctgg gcttctgcta cctgcggtgt
    14881 taatggctga gctggtgtgc cgggggcatc atcaggctgt gctgttgtga gttgcgcaga
    14941 agtcagtttt gtgaagagcg ttggtttctc tgccggtaac acggttgacg gcacatcagt
    15001 cactttgggc gtattgtcaa aacccggcaa catcgcaaaa agggcgctca ggcttgcggt
    15061 gacgtcttca ttcagatcat ccgctttttc gtcttttgtc gtatttttgt cagccaccgc
    15121 agccaacgcc atcgtctgag cggtggttaa cggtgttgat gtggattgtt cgtcgttgat
    15181 gacaggcggt gtttcatcca cagggatcag taaattagct tgttgcgcgt cggaaacaat
    15241 atcgctgatc agcggctcgc cttttgtcgt gggcttatct gttgccacca gcaactgggg
    15301 ggccgctttg tcggtagttg tctcgcctgc taatgcttcg ctcaacaacg cgagaaaatc
    15361 ttgtgcagca tcgctggctt tgccgccagg caatgtggtg gtgtcaacgt cggcggtaat
    15421 caagggcgct aagcgaatca ttcaggtttc ctcatggcgg cgcgctgggc gaactcatcc
    15481 atctttttct gatcgaggcg gttttctgca agcagtgccg ccgtggattg ccgttcctgc
    15541 agtgtctgcc aggcctgcaa acgttgtttc ttttctcgcc agctgttcag ggcaatgtca
    15601 actttctgcg tccactgatt aagttgctgg cgatgctgag taatggcttt ttccagcgtc
    15661 tggataaact gctgatagtt gatccagcgg ttgctggtca tcccggcact catatcgctg
    15721 ttgaggttat tgcgatattc attctgataa tcgatcagca ttttgagctg ttcttccgcc
    15781 tgctgacacc cgcgacgcat ttcacccagc aggcgcgcgg catcctctac ctctttttct
    15841 gccagatctt tcagggtcgc cagcgcgcca tgttctgcca tctgccgtta tctcctgggt
    15901 tatgacactg tcgggaaaat acgctccagc ccctggagag acgcttccca gtccgcgcgt
    15961 tcaaaaatgc cttgttgcaa atagccctcc agctgcggcc acagggcgat ggctttgtcg
    16021 agcatcggat cgctgccttt ggcatacgcg ccgacgctaa ccagatcgcg gttacgctga
    16081 aaactcgaca acagctgttt gaaggtgcgc actcgcgcgt aatgttgctc actgatcaac
    16141 gccgtcattg cgcggctgat cgacgcttca atatcgatag ccggatagtg cccggcttcc
    16201 gccagtcggc gagacagcac aatgtgaccg tcgaggatcg cccgcgcgga gtctgcaatg
    16261 gggtcctgct ggtcatcgcc ttcagtaagc acggtataaa acgcggtaat cgagccgccg
    16321 ccgctaatgc catttccggc acgctcgacc agtgccggta atttggcaaa caccgacggt
    16381 ggataacctt tggtggcggg tggttcgcca atcgccagcg caatttcacg ctgggccatc
    16441 gcgtagcggg tgagggagtc cataatcagc aacacatgct gaccacgatc gcgaaaatct
    16501 tcggcaatgc gcgtggcata ggcggcacct tgcattcgca ggagcggaga cacatccgcc
    16561 ggagcggcaa tcaccactga tcgtgcacgc ccttcggcac cgaggatatt ctcaataaaa
    16621 tcttttactt cgcgcccacg ttcaccaatc aaacccacga caatgacatc ggcgcgggtg
    16681 taacgtgcca tcatccccag cagcacactt ttaccaacgc cggacccggc aaacagcccc
    16741 atacgctgcc cacgcccaac ggtaagcagg gcattgattg ggcgcacgcc ggtgtccagc
    16801 acatgttcaa tcggtgtacg ttgcaacggg ttaaatggcg gggtaatcag cgcaccggtt
    16861 tccgtcgtat cgggggaggg caggccatcg agcggtttac cgctgccgtc cagaacgcga
    16921 cctaataacg ccggaccgag cggcaactgc ttgccgcttt gcagtccttc agccgaaatg
    16981 tttttggcat aaacccgcgc gccgggcagg acaccttcca cttcctccag cggcattaaa
    17041 aacagccgtt gaccgttaaa gccaacgact tcgctttcta cttcgtgcgt ttcgctgccg
    17101 ttctggcgtt caatgacaca ggttgcgccg agcggcaatt gtaatccggt ggcttccagc
    17161 accagcccgg tagcgcgggt taatcgcccg tagcgacgta ccgcaggcaa ctgcgccatt
    17221 ttggcttcaa agttatccag cgtggttagc cagcgagtca ggcgcgtggt cattacacca
    17281 ctcctggtgc tgccagacgg cagagttctt gccagcgagt caggcgcgtg gtcattacac
    17341 cactcctggt gctgccagac ggcagagttc ttgccagcga gtggcgacac tggcgtcgag
    17401 atcgccttca tcggcggaga ctttacagcc gccaggatgg agggtgggat cgccccgcaa
    17461 gcgccagcca tgcaaactta aggtagcgcc gagcatatca tccacacgtt gcagatcatc
    17521 cgggtgcacg cgcagctgtg gtttaccgct gaataacggt tcttgctgca acaactgttg
    17581 gatctgtttg atcagtgccg agttatccac cgttggcgtc tgaccgatga cctgacgtgc
    17641 cgcctccagc gccatctgca tcaggcgcga cgctatcaca ctatcaagtg catcaagggt
    17701 agtttgaaat tcgctgacca gttgctgcat ccgggcatga attggcgctt gttgagactt
    17761 cgcctctgcc agaccttgct ccagcccctg ggccagtcct tcctgatagc cctgcttatg
    17821 accttgctgg cgaccttcgg caatacccgc ctgataacct tgctcatggg cctgcatttg
    17881 cagttgcgcc agttgctgct caaggctggg ttcagcctct tcaatgatgg tttcttccga
    17941 ctcgaccatg ggcacaaact ctgcctgtgg tggcgcgaga tcgtccggcg tccaggtttt
    18001 ccagggcaga ttatcagaca taggtatcct cgccgctgcc aattaccatc tcgccagttt
    18061 cggcaaggcg gcgcacaatc agcagaatcg ctttctgttc gttttccacc tgcgacagac
    18121 gcaccggacc acggttggcg agatcgtcgc gcagaatatc ggcggcacgc tgcgacatat
    18181 tgcgcaggaa tttctcgcgc agtggctgct cggccccttt cagcgcgatc aacagcgatt
    18241 cggaatccac ttcctgcaac agacgctgaa tgctgcgatc gtcgacatcc accagatttt
    18301 cgaacaggaa catctcgtcg atgattttct gcgccagctc gccgtcgaat tcacgcacgg
    18361 cggtaataac ggcttcttcc tgctgagttt tcatcaggtt gataatttcg gctgccgttc
    18421 tcacgccgcc cattttgctg cgcttgagat tctgaccgtc gagcaagcca ttcagtactt
    18481 cggtcagttc cgccagcgcg gctggctgca cgccgccaaa ggtggcgata cgcaacatca
    18541 cgtcgtggcg cagacgttca tcgaacaacg ccagaatatc ggcggcctgg gcgcgcttca
    18601 gatgcaccag aatggtggcg ataatttgcg gatgctcatc acgaatcaga tcggcggcgc
    18661 tctgcggctc cataaagttg agcgtttcaa taccgctggc ggtatcgcga gtttcgagaa
    18721 tatcttccag caggctggcg gcacgttcct cacccagagc tttgaccaat accgaacgca
    18781 gataatcgtt ggcgttgata ttcagtgcgg caaactgttc agcttcttgc tcaaactccg
    18841 ccagcacatc ggttagctgc ttgttggaga tctgcgtgac gttcgccatt gcagcgctca
    18901 gggtttgcac ctcgcgctgg gagaggtgct tgaacacttc tgccgcccgg tcttcgccaa
    18961 tggtcatcag caggatgacg cttttatcgg tgcctgtcag gttactcatg atcgttattt
    19021 atccactggc gaatgaccag cgccaccacg cgcggatcgt tatcagacat ttcacggata
    19081 cgctggctca tgacttctgc ccccagacgt tggttagcgc gccgttgttg gagttgttcg
    19141 tctttgctca ggcggacttc caccgcatct tccacttcct cgcgggcctg cgcctgttgc
    19201 tgtacagctt tcatcgcctc agcgcgacgt gttagctgcg ggcgtaccgc tttacgccac
    19261 agcagccacg ccaccagcag taccagcaac cagcgaccgg cagccagtaa ctgatcgata
    19321 aacgcttgct gttgccagaa cggcagttct ccgccgcttt cgtcactact attgaacggc
    19381 gagttaacga cattgagcga gtcaccgcgt ttttcagaaa agcccatcgc ctcgcgggtc
    19441 agatcttcaa tttgtttcat ctgttcgttg ctgagaggca atggtttacc gtctggcaag
    19501 gttttgtaat tcaccactac cgcgactgac agacgttgca catcgcccac gttcattttg
    19561 gtatgacgaa tagtgcgatc gacttcgtag ttactggttt cattccgctg tgtgctacgc
    19621 ggcccactat tgctggtggt gctcgcctgc tgctggcggt tattttgatt tgccggaggc
    19681 gtgctgattg gcgcgttatt cgcaggagcc ggttgattcg acaacgcccc cggtacgccg
    19741 cccggataac cggaaccgct ttgctcgctc tcattaagct ggcgtgaacg aagcgccgcc
    19801 tgagattcat caccattagg gcgatactgt tcttccgttt gttctttact ggcgaagtcc
    19861 agttgcgccg tgacctgggc gtgaatatta ccgttaccaa caataggcga caggattgct
    19921 tcaatacgcc gctgaatacg gccttcgaca tcgctggcat atttcaattg agcgtcatta
    19981 agatcgcgcc agctggtatt ggactgggtt aacagatgtc cgccctgatc caccagcgtg
    20041 acgtttcccg gcggcagacc agcaacggcg ctggaaacca gatgcacaat ggcgctaatt
    20101 tgcccttcat cgagtgcgcg gccgggtaac agatttaccg tcaccgatgc agaaggggat
    20161 ttttgttcac ggacgaataa agacggtttc ggcattgcca gatgtacgcg cgcccctttt
    20221 accgggccga tagtttcgat ggtacgagaa agctcgcctt ccagcgcccg ctgataattc
    20281 acctgttcgc tgaactggct gataccaaac ttttcctgat cgagtagttc gaaaccgacc
    20341 gcgccgcctt tgggcaaacc ttgttgcgcc aggcgcaggc gcagctcgtg aactttatct
    20401 gccggaactt caatagcgcc gctggcttcg ctgaagcggt aaggaatatt catttgcgtc
    20461 agttggctga caattgcgcc accatcctga tcggaaagat tgctgaataa tgtgcggtag
    20521 tcgggggctt tcgcccacag gatcagtgcg accatgaccg ccactgccgc ggaaccggca
    20581 acaatcaatg gaattttcgg attcgcacgc aggcgattaa gccactcaag agattttgtc
    20641 tgggctgcag tcgcattcat cgcgcacctc gtggctgatt atggacggcg ttattggcaa
    20701 agtggaacaa gactcactcc cgggtcagca aactcgaaaa attgacgctc ccattatttg
    20761 tgctttcgtt tttttctatc cgccaataaa cccgtttttt tgttgctatt tagcgccttt
    20821 gtcttattga cttactggta ggctttgcta ccagaaatta tccaggagat gaaaatgtca
    20881 gcgatacagg ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt
    20941 gcgcaggaat cactgccgca accgaccatt agttttgccg ggcagctgca cgccgcgctc
    21001 gatcgcataa gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt
    21061 gaacccggcg tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg
    21121 caaatgggga ttcaggtgcg taataagctg gtggcggcat atcaggaagt aatgagcatg
    21181 caggtatagg ttgtgtaacc tgttgttaat gacatccgat ctcaccgcgt ggggcatgga
    21241 tggggcaaac ttactcatta tctggtttga aatggatacc tgatcctgaa agttttctgc
    21301 catccacgat ccgtatacac gataaacccc ctgaacatca gagtgtctca tttgacacat
    21361 caccacgtcg gatgttgtta cgaccacaga cgcaacagta acctctggat aattttatct
    21421 ttgttgatgt ttttattcat gttccgggct atggatatga gcgattt
//
LOCUS       DAZFKY010000073        20805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFKY010000073 DAZFKY010000000
VERSION     DAZFKY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1650)
                     /gene="actP"
                     /locus_tag="ACHKCL_002402"
     CDS             complement(1..1650)
                     /gene="actP"
                     /locus_tag="ACHKCL_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5763359.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1647..1961)
                     /gene="yjcH"
                     /locus_tag="ACHKCL_002403"
     CDS             complement(1647..1961)
                     /gene="yjcH"
                     /locus_tag="ACHKCL_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5763360.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2162..4120)
                     /gene="acs"
                     /locus_tag="ACHKCL_002404"
     CDS             complement(2162..4120)
                     /gene="acs"
                     /locus_tag="ACHKCL_002404"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5763361.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHKCL_002405"
     CDS             4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHKCL_002405"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5763362.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHKCL_002406"
     CDS             5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHKCL_002406"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5763363.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHKCL_002407"
     CDS             6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHKCL_002407"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5763364.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHKCL_002408"
     CDS             7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHKCL_002408"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5763365.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8201..9919
                     /gene="nrfE"
                     /locus_tag="ACHKCL_002409"
     CDS             8201..9919
                     /gene="nrfE"
                     /locus_tag="ACHKCL_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5763366.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHKCL_002410"
     CDS             9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHKCL_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5763367.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHKCL_002411"
     CDS             10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHKCL_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5763368.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11230..12543
                     /gene="gltP"
                     /locus_tag="ACHKCL_002412"
     CDS             11230..12543
                     /gene="gltP"
                     /locus_tag="ACHKCL_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5763369.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12721..13410)
                     /gene="yjcO"
                     /locus_tag="ACHKCL_002413"
     CDS             complement(12721..13410)
                     /gene="yjcO"
                     /locus_tag="ACHKCL_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5763370.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13504..15651)
                     /gene="fdhF"
                     /locus_tag="ACHKCL_002414"
     CDS             complement(13504..15651)
                     /gene="fdhF"
                     /locus_tag="ACHKCL_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15232..15234),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO5763371.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15849..17315)
                     /gene="mdtP"
                     /locus_tag="ACHKCL_002415"
     CDS             complement(15849..17315)
                     /gene="mdtP"
                     /locus_tag="ACHKCL_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5763372.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17312..19363)
                     /gene="mdtO"
                     /locus_tag="ACHKCL_002416"
     CDS             complement(17312..19363)
                     /gene="mdtO"
                     /locus_tag="ACHKCL_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5763373.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19363..20394)
                     /gene="mdtN"
                     /locus_tag="ACHKCL_002417"
     CDS             complement(19363..20394)
                     /gene="mdtN"
                     /locus_tag="ACHKCL_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5763374.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20413..20688)
                     /gene="ytcA"
                     /locus_tag="ACHKCL_002418"
     CDS             complement(20413..20688)
                     /gene="ytcA"
                     /locus_tag="ACHKCL_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5763375.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4688 a         5508 c         5680 g         4929 t
ORIGIN      
        1 ttaatgcgcg cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg
       61 gaacagttca cgctcacgcg cgccttccgc tgagttatcg gttgccgaga agaaccagat
      121 gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga agatggcttt
      181 ttcgtgaccg aggatctgta cccaaatcgt cgggccgagg atcatcagta ccactgcggt
      241 aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg accagtacat
      301 agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca gccccaccat
      361 aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca cacccaaaat
      421 caggacggtg attttcgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac
      481 gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc agcgtcagac ccgcaaccac
      541 cgcgaggata gtggcgaaag caaccgctga aataaagccg aggaacaggt tgccgcccac
      601 cgcattggcc aggtgaactg ctgccatgtt gttaccacca atcagatggc ccgccgcgtc
      661 tttatattcc ggattcgcac cgaccagcat gatcgcgccg aagccgataa taaaggtcag
      721 gatatagaag tagcccataa atccggtagc gtagaacacg cttttacgcg cttcgcgggc
      781 atcgctgacc gtaaagaagc gcatcagaat gtgcggcaag ccagccgtac caaacatcag
      841 tcctagcccc agagagagtg cggagatcgg atctttcacc agtccgcccg gtttcatgat
      901 gtcgacacct ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc
      961 gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt
     1021 gataatctgt acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag
     1081 cacgccgacc agcaccactg caatgtgata gttaaggcca aacagcagct cgatcagttt
     1141 acctgcaccc accatctggg cgataaggta aagcgccacc accaccagag aaccacaggc
     1201 cgaaagaata cgaatcggcc cttgtttcag acgataagag gctacatcgg caaaggtgta
     1261 acgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc agcccaccag
     1321 gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagtg cggagatccc
     1381 caaaaatgag gcggcggaca tatagtcacc ggcaatcgcc agcccgttct gaaaaccggt
     1441 gatattgccg cctgcggtgt aatagtcatt acgcgaacgt acgcgttttg acgcccagta
     1501 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1561 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1621 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
     1681 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccagtgag aacaaaggag
     1741 atcactatta ccccaacacc gactggaata ccgcgtgtga cgctggtgtt cggattcagc
     1801 ggggtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttat acgccgcatc cggcaatcaa tgcctgatgc
     2101 gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga ctcgcatccg gcaattgtgg
     2161 gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgact acgccaggat
     2221 cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa
     2281 tacggcgcat aattttgccg gagcgggttt taggcaggga gtcggtccag tgcagcacgt
     2341 ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct gcgtacagtt
     2401 ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa
     2461 tattgtgcgg aataccgacg acagcggctt cggcgatttt cggatgcgcc accagcgccg
     2521 actcaatctc cgccgttccc agacggtgac cggagacgtt cagcacatcg tccacacgcc
     2581 cggtgatcca gtaatagcta tcttcatcac gacgcgcgcc gtcgccgctg aaatacatat
     2641 ttttgaaggt ggaaaaatag gtctgctcaa aacgttcgtg atcgccaaac agcgtacgcg
     2701 cctgacccgg ccaggagtcg gtgatcacca ggctaccttc ggtagcccct tccagcgggt
     2761 taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg
     2821 ctttcagctc ggtagcgcca ggcagcgggg tgatcatgaa accgccggtt tcggtctgcc
     2881 accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac cactcccacg
     2941 cttccgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa cggtcggtgc
     3001 cttcgatcgc tttatcgcct tccgccatca gcgcgcgaat cgccgtgggc gcggtataga
     3061 gaatattgac ctgatgcttg tccaccacct gtgccatacg ggcaggcgtc ggccagttcg
     3121 gtacgccttc aaacatcagc gtggtcgcgc cgcaggccag cgggccgtac agcaaataac
     3181 tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcgccc ggatgataat
     3241 caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta gtgtgcagta
     3301 cgccttttgg ttttccggta gaaccggagg tatagagaat aaacagcgga tcttcggcgt
     3361 tcatctcttc cgcctggtgc tgatcgctgg cttgctcaac ctggtcgtgc caccacaggt
     3421 cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc acatgctcta
     3481 cgctggtgac gttcgggttt tttagtgcgt catcaacgtt tttcttcagc ggaatactac
     3541 gcccggcgcg cacgccttcg tcggaagtga tcaccagtcg tgagttggaa tcgataatgc
     3601 gcccggcaac cgcttccggc gagaagccac cgaaaattac cgaatgcacc gcgccaatac
     3661 gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa atcgccacca
     3721 catcaccttt tttaatgccc agcttgagca gggtattggc gaagcggcag acgtcgcggt
     3781 gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg
     3841 cggtacgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc agattcagcg
     3901 tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggtg tttttcacct
     3961 tctggtacgg tttgatccag tcgagaattt ttccctgttc gccccagaag gtatcaggtg
     4021 cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg caacggtctg
     4081 cgatgttggc aggaatggtg tgtttgtgaa tttgactcat gcttttgttc tccttgtagg
     4141 atgttaataa tatgtggcat aagcgttaaa tgtaggggta ttggcagttt tgtttagtat
     4201 ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt aatttttgaa
     4261 ggtcagcaac aaaagttgat taattctttg aggaacatgc agttatgcat gctgttgaaa
     4321 aagaggaaga tactgactaa ctctaaagtg gtattttaca tgcacttaca attgattaaa
     4381 gacaacattc acagtgtggt tatttgttac acttaggggc gagcaatgtc atgacagtgt
     4441 aggtgcggtt actgtcgtga aaaacaataa aaaccgccat tgcaacaatg gcgcaattcg
     4501 gatgaagccc ctatgacaag gataaaaata aacgcacgcc gtatcttcag cttattgatt
     4561 ccttttttct ttttcacttc tgttcacgct gaacaaacgg ctgctcccgc aaaacctgta
     4621 actgtggaag cgaagaatga aacctttgcc ccgcagcatc ccgatcaata tctctcctgg
     4681 aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc acggctggtg
     4741 atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg acatgccttt
     4801 gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc tgaagatggt
     4861 ccgctaccga tggcgtgctg gagttgtaaa agcccggatg tggcgcgtct gatccagaaa
     4921 gacggcgaag atggctactt ccacggtaaa tgggcgcgcg gcggcccgga aatcgtcaac
     4981 aacttaggtt gtgccgattg ccataacacc gcctctccag agttcgccaa aggcaaaccg
     5041 gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg taaacctttt
     5101 gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca tgtggagtat
     5161 tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat gaaagtcgaa
     5221 aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc cctgtcgaaa
     5281 acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg cattcacggt
     5341 aaaaacaacg tgacctgtat cgactgccat atgccaaaag tgcagaacgc cgaaggcaaa
     5401 ctctacaccg accataaaat tggtaatccg tttgataact tcgcccagac ttgtgcgaac
     5461 tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa gcagtcgatt
     5521 aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga agcgaaagcg
     5581 gcgctggacg caggtgcgac ggaagccgaa atgaagccga ttcaggacga tatccgtcat
     5641 gcccagtggc gttgggatct ggcgatcgct tcccacggca ttcatatgca cgcaccggaa
     5701 gaaggtctga ggatgctcgg tacggcgatg gataaagcgg cggatgcacg caccaaactg
     5761 gcgcgcctgc tggcgactaa aggcatcacc catgaaatcc agatcccgga tatctcgacc
     5821 aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc cgagaagcag
     5881 gacttcatca aaacggtgat cccgcagtgg gaagagcagg cacgtaaaaa cggtctgtta
     5941 agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg aatatgagcg
     6001 tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg agcctgaacg
     6061 gaattaccgc tacccctgcg gcgcaggcat ctgatgatcg ctacgaagtt acccagcagc
     6121 gtaacccgga tgccgcctgt ctggactgtc ataagccaga taccgaaggt atgcatggca
     6181 aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc aactgtcacg
     6241 gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt aacgagccga
     6301 tgtacaaggt tggagagcag aacagcgtct gtatgtcctg ccatctgccg gaacagttgc
     6361 aaaaagcgtt ctggccgcac gatgtccacg taaccaaagt ggcgtgcgcc agctgccatt
     6421 ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg attaagattt
     6481 gcgtcgattg ccacagcgat cagcgcacca atccgaactt taacccggcg tctgttccgt
     6541 tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg tcggcgtgct
     6601 ggcagccgtc agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata tcaatggggt
     6661 gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct gtatggatgc
     6721 ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta ttcgtagcga
     6781 gccgcagggc gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt gccagcactg
     6841 cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgca tcttttcgcg atgctgccag
     6901 tgggattgtc gatgttaatc cggatctctg cgtcggttgt cagtactgca tcgccgcctg
     6961 tccgtaccgc gtgcgcttta ttcatccggt cacgaagaca gcggacaaat gcgatttctg
     7021 ccgtaagacc aatttgcagg ccggtaagct tcccgcgtgc gttgaggctt gcccgacgaa
     7081 ggcgctgacg tttggcaatc tggacgatcc caacagtgag atttcgcaac tgctgcgcca
     7141 gaagcccact taccgctaca agctggcgct gggaaccaaa ccgaagctgt accgcgtacc
     7201 gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt tgaatcgctg
     7261 gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc cggtctggtg
     7321 acgctggccg tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga cagcacgttg
     7381 ctgcgcacca cgctgattgt cggacctggc gcggtgatcc tcggtctgtt gagcctcgtc
     7441 ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag ttttacttcg
     7501 gtgatgtcga tgggggtgat gctgtttcag ctttacatgg tggtgctggt gctgtggctg
     7561 gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc gaagctgggg
     7621 atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga aacattgatg
     7681 ctggtgctgg cggtgttgtt gggggcttat accggttttc tgctgtcggc gctgaaatcg
     7741 tatccgttcc ttaataaccc gatcctgccg gtgctgttcc tcttctccgg catctcgtcc
     7801 ggtgcggcgg tggcactgat cgccatggcg atacgccaac gcagtaaccc gcattccacg
     7861 gaagcgcagt ttgtacaccg tatggaaatc cccgtggtat ggggtgaaat cttcctgctg
     7921 gcggcgtttt ttgtcggtct ggcgctgggc gatgacggta aagtgcgtgc gctggtggcg
     7981 gcattgggcg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg gctggggctg
     8041 attgtgccaa tgttgttcaa accgtgggtc aatcgcagtt ccggcattcc tgccgtgctg
     8101 gcggcgtgtg gggccagtct ggtcggcgtg ttgatgttgc gatttttcat tctctacgcc
     8161 gggcagttaa cggtggcgta agccagaaca gaggtggttt ctggacgtat tccttcctga
     8221 agtcggtttt ctggcgctgt tgttaagtct cggggtcaac gtgttgaccc cgttgacggc
     8281 ctttgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca tcggcattct
     8341 ggcacagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt ttctcatcag
     8401 cgatttctcg gtcatttatg tcgcccaaca tagctacagc ttgctgtcgt ggaaactcaa
     8461 actggcggcg gtgtggggcg gtcatgaagg ttcgctgctg ctttgggtgc tgctgctttc
     8521 cgcctggagc gcgctgtttg cctggcaata tcggcagcaa accgatccgc tatttccgct
     8581 gacgctagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtag tgctgtggtc
     8641 cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca atccgatgct
     8701 gcaacatccc ggtcttatct ttcatccacc gctgctttac cttggctatg gcggtttgat
     8761 ggtagcggcg agcgtggcgc tggcgagtct actgcgcggc gagtttgatg gtgcctgcgc
     8821 ccgaatttgc tggcgctggg cgttacctgg ctggagtgca ttaactgcgg ggatcatcct
     8881 cggttcctgg tgggcctact gcgaactcgg ctggggcggc tggtggttct gggatccggt
     8941 ggaaaacgcc tctttattac cctggctttc tgccactgcg ctgctgcaca gtttatccct
     9001 gacacgccag cgggggattt ttcgccactg gtcgctgttg ttggcgatag ttactctgat
     9061 gctgtcgctg ctgggcacct taattgtccg ttctggcatt ctggtttcgg ttcatgcgtt
     9121 cgctctggat aacgttcgcg ctgtgccgtt gttcagcctg tttgcactga ttagccttgc
     9181 gtctctggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc gtttttcggg
     9241 gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag tgctactgat
     9301 cgtgctggtg ggaacgcttt atccgatgat ttacggcctg ctgggctggg gacgtctctc
     9361 cgttggcgca ccgtatttta accgcgcgac gttaccgttt ggtctgttga tgttggtggt
     9421 gattgtgctg gcgacgtttg tctctggcaa acgcgcgcag cttccggcgc tgctggcgca
     9481 tgcgggcata ctgttatttg ccgcagggat cgttgtctcc agcgtcagcc gtcaggagat
     9541 cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct tccgttttga
     9601 gcgcctcgat ctgcaagcca aaggcaatta caccagcgaa aaagcgatag tggcgctgtt
     9661 tgaccatcag caacgcattg gtgaattaac gccggagcgg cgtttttacg aagctcgtcg
     9721 tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt atgcggtcat
     9781 gggggagaaa actggagcgg atcgttacgc ttttcgcttg tatgtacaaa gcggtgtgcg
     9841 ctggatctgg gggggaggat tgttgatgat tgcgggcgca ttgttaagcg gatggcgggg
     9901 gaagaagcgc gatgtataaa gggcttctca cgctactgct gttatttacc tgttttgcgc
     9961 gcgctcaggt tgtagacacc tggcaattcg ccaatccgca acaacagcaa caggcgttaa
    10021 atattgccag ccagttacgt tgtccgcagt gccagaatca aaacttactg gaatccaacg
    10081 cgccggtggc agtcagtatg cgccatcagg tttacagcat ggtggcggag gggaaaagtg
    10141 aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc tataacccac
    10201 cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta ctgctgatgg
    10261 cactgatcct ctggcgagtg agggcgaagc gatgaaacag cctcaaatac cggtgaaaat
    10321 gctgacaaca ctcacgatat tgatggtatt cctctgtatc ggtagttatc tgttaagccc
    10381 aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac atcagtttgc
    10441 cagccagcaa aacccggaag cgcagcttca ggcattgcag gataaaatcc gcgctaatcc
    10501 acaaaacagc gaacagtggg cgttactggg cgagtactat ctgtggcaaa acgattacag
    10561 caattcgctg ctggcgtacc gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta
    10621 tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg cccagactcg
    10681 cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct
    10741 ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat ggcaaaaagt
    10801 gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga ttaatatggc
    10861 gaaattgttg cagcggagat cggattaatc atctggtgat gaagattatg ttattgagct
    10921 gcataaaaaa taatcgaatg aacatatgcc aaaaataatc actaatcagt attattgcag
    10981 attaaacaaa taaaaatctt tccataacaa atggttattc attaatcctg ccataaactc
    11041 gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg caacataccg
    11101 cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa cacaattcat
    11161 gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa
    11221 gtcattcata tgaaaaatat aaaattcagc ctggcctggc agattctgtt tgctatggtg
    11281 ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga ctggctggtc
    11341 gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat gattgttgtg
    11401 ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc
    11461 gggcgtattg gcgcgaaaac cattatctac ttcgaggtga tcaccaccgt tgccatcatt
    11521 ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat gtcgcagctg
    11581 gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag cagttcccac
    11641 ggcattatgg gcacgatttt gtcgctggtg ccgacgaata ttgtggcgtc gatggcgaaa
    11701 ggcgaaatgt tgccgatcat cttcttctcg gtgctgtttg gtctggggct ttcctccctg
    11761 cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga aaccatgttt
    11821 aaagtgactc acatggtgat gcgttatgcg ccggtgggtg tgtttgcgct gattgcagtg
    11881 acggtggcta actttggttt ctcttctctg tggccactgg cgaaactggt gctgctggtg
    11941 catttcgcca ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg cctatgcggg
    12001 ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc gtactccact
    12061 gccagctctg aaagcgtact gccgcgaatt attgagaaga tggaagccta cggagcaccg
    12121 gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt ttaaccttga tggttcgacg
    12181 ctgtatcaaa gtattgccgc tattttcatc gctcagctgt atggcattga cctgtccatc
    12241 tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat tgctggcgtg
    12301 ccgggcgtgt cgtttgtggt gttgctggca acgctgggta gcgtaggtat tccgctggaa
    12361 ggtctggcgt ttattgctgg tgttgaccgt atcctcgaca tggcgcgtac tgcactgaac
    12421 gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa atttgaccgc
    12481 aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa
    12541 taattgaaga ttgccgggga tatcccccgg caatgtgtgg atgcctgatg cgacgctggt
    12601 gcgtcttatc aggcctacgt cagacagcgc tagcgtgatt ttgtaggtct gataaggcgt
    12661 ttacgctgca tccgacatca acgcctgatg cgacgcttaa cgcgtcttat caggcctacc
    12721 ttaaccgtta gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct
    12781 caggttcaac cagtgcagcg ccttttgctt gttcttctcg ataaaacctt cttcaccgtt
    12841 taagaacatc attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg
    12901 tttgaaatac caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat
    12961 caaaccaagc agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcgtt
    13021 ttctaacagc gaaatcgcct ttggataatc cggtttaccc gcttgagtat ttaccagaat
    13081 atgcgccagc gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac
    13141 ttttgcttgt ggatagtcca gactgaccgg attggtgatt ttgatctgcg ccaacagcgc
    13201 gcaggcatca gcatcaccgc tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa
    13261 atcgccggag ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc
    13321 tgctgccttt aaatactggc tgccaggctc ggagtcgttg gcgtgggcaa agaatgtcaa
    13381 aaacaacatc aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc
    13441 agtataaaga gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggact
    13501 gtattacgcc agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac
    13561 gtactgctcg gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta
    13621 ttccggcgtt ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat
    13681 ccaccactgg taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg
    13741 ggtgataatt ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatactcag
    13801 acgtttggcg tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag
    13861 tgccgcacag ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga
    13921 cagtaccatc gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa
    13981 gaactgcgcc agaccgttcg gggtgtcaaa cttctcttta aacagataag aagtcccctg
    14041 atcggcatct gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttctc
    14101 gtaagtcgca ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt
    14161 gttgttgtag tgcatcggat aacccatacg ggtggcgatt tcactgatga tttgccagtc
    14221 cgttttcaga tcccatttcg gttcaactgc tttgaagaaa cgctggaagc cacggtcagc
    14281 cgcagtaaac acgccttcat gctcgcccca cgacgttgac ggtaaaataa catccgccgc
    14341 cgacgcggtt ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc
    14401 tttacgtact gccgacagct ccgcgtcagt ttgtagcgga tcttcgccca taatgtacgc
    14461 ggcacgcact tcgccatgcg ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg
    14521 agcaggcagg ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt
    14581 tacgtactgg tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac
    14641 gttgttctga ccacgaaccg ggttaacacc cgcatgtggc ttaccgaggt taccggtcag
    14701 catcgcgagg ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc
    14761 catgccccac aggatggcgg cgcttttcgc ctgggcatac atccgtgccg cctgacgaat
    14821 ctcactggcg ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat
    14881 tttacgatac tcttcaaagc cttctgtacg ggaagcgacg aatgctttgt cgtacagatt
    14941 ttcttcaata atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag
    15001 tgcaatgtgc atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat
    15061 tttcgccccg ttacgtttag cgttaattac gtgattcgcc acgattgggt gggaatccgc
    15121 cgggttgtac ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct
    15181 cattgcgcca ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg
    15241 agcgcagcag tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata
    15301 gttggtttcg ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta
    15361 cttctctttg atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac
    15421 aggttcgagt ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag
    15481 gatctgggta tcgttaatga agtcccagcc ataataaccc ttcagacaca gggtaccctg
    15541 gttggttttc ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt
    15601 gattttgcaa cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc
    15661 tccagttaat caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca
    15721 ttttatttgg ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa
    15781 acaggctgtc gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacacgg
    15841 cagacatttt attttttctc gacgacggga gccgcctgat acccgctgcc cagcgatttc
    15901 atcaactgaa tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcttcg
    15961 gcaagcaccg gcaaccgggc ttcggtagcc tgtaagcggc tggttaagcc gcgctgatag
    16021 gcggcctcgg cagcgcgctg ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct
    16081 cgctcgtcgt tgagcgtttg cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc
    16141 agtactgact ggttgtaacg ttcaatcatc atgttgctgg cggcgcgagt gccttcgaga
    16201 ttggcattca accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac
    16261 tggcgactgg tttttttgaa taaggtatcc aggtggatgg agtccagacc gaaaaacgct
    16321 ttgatatcaa agcttgggta gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc
    16381 tgaacatacc agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt
    16441 gtcgccggaa taccggtctg gactcgtggt aatgccactg gtttgatctc cggcatatcg
    16501 ctggctcccg cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgcccttta
    16561 acggcagcaa tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact
    16621 tgcgcttcca gaccgtgcgc cactttactt tggtgcgctt tcaccgcgta atcaatcaca
    16681 tcgcgagttt gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc
    16741 tgcgctacgc ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta
    16801 tgcgcgccaa tggcggcggt aaccgctgag cgatgcacac cccacaaatc aagatccagt
    16861 ccggcaaaca aaccgactgt ggcttccgta tagtacggcc cgtccatacc cagtgctggc
    16921 gcatccatcg cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc
    16981 cctaacgctg cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct
    17041 ttttcttccc gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc
    17101 agcgcatcca gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag
    17161 ctggcaagat gaatatcatc ggccagtttg atttgttccg gtttgagctg ttgatgaggc
    17221 gcagaatcct tgcgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg
    17281 cacaacagca gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg
    17341 tggcgggcgt taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt
    17401 gttgctcctg ttttaataag gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg
    17461 ttatttgagg agaacggctg agtgcggttg ccagaccggc agcgtatttt tccagggcgt
    17521 cggcaaactg acctgctttg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc
    17581 aggtggcatc ccaggcgtga agaagatcgc ggccctgatg aataaccgtt tgcgaacgtt
    17641 caatcagtaa ggcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca
    17701 tctcttcaca ggcattaaac gccgcatgca gacctatacg aatttgcaga taagtgcgca
    17761 gagccgtgac ttcctgctgg cgtgggatcc gcattacttt acttagcata cccagcgcgc
    17821 cagccagttt ttgcggcagc gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca
    17881 ccgcagacac cacggtacca atgatgatac ccagggcgcg atcgcgaatt tccaccaggt
    17941 cgtacaccgg accaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg
    18001 tgccgatata agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg
    18061 gtgccagcac aaacagcaat tcgacaatat tgtccagcca gggcatgacc agtagcgtga
    18121 ataacagcgc cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt
    18181 acgatgaacc gacattcgga ttagcgacga tcacgcaggt cagcatacag gtgtgaatgc
    18241 cttcccagtc cacgccgctg tagaaggtgt aacagatcaa acaggcgagc agcgttttta
    18301 ccgcgtagcg catatagtct ggattggtaa aagcatcagc gaccattgat ggcggtttgg
    18361 cggcgggcgt tggcggcgta ttcgggtcca tctgacccag ttgtaacaac gtctggcaga
    18421 tattctccag gttacattcc cgtgccgcca tcgcttcact ttcactgatc cgccagtcgc
    18481 tttgccagca ctgaccttcg gtaatggcat gctgcagctt gttgatttct gaagctaatt
    18541 tttgtcggaa ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca
    18601 gcgtcgagta aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctttgagttc
    18661 gccagttggc atcgtccgcg aggcaaaaga cattgagttt ttgcagcgcc agcgcctctc
    18721 tttcaatccg cgtttcgggt agcggtgcga ggctgtccgt caggtggcta atggcatcat
    18781 caagccgatc attaagcgct tgatgcattt gcgaaatggc acgactggga aaccacagta
    18841 cgccgattaa cgtcattaat aacgtcgggt agaggccaac aacgatacac cacagcgtta
    18901 agcgcacgac cacttccgga tagtcgagca tggcggggaa ggtttgccca taaatggcga
    18961 caatggcgac agcgaaaaag accagtccca accgatgggt gcgcatcaaa aacatgcagc
    19021 ccatcaggat cggcccggcg atgatcaagc ggatcaacgg ttcgccgtat gaccatttgt
    19081 agatcaaaaa caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa
    19141 atttggtgta aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca
    19201 caaaagggat ctcaaaagtc atcgagatca gaatcaccag caggcagcct acccagagtt
    19261 gcacggtctg cggcacgcga cctggtcgcc gctcgcttaa ctcttcatga aagaacgcca
    19321 gcagcctgac caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt
    19381 agcgactgcc gaagcgccga tgcggaacat ttccgggtca ggtttatcaa ccatgatttt
    19441 gaccggaaaa cgctgggcaa cacggaccca gttaatagaa cgagaaactt tcggcaggcc
    19501 gcccagcacc aggccgccgt catccggtag cacgccgtag ccaattgaat ccactttacc
    19561 ctcgaaagtt ttgccgctgt cactcatcag gcgaatcgtt gcaggtgtac ctgagcgaat
    19621 atttttcaga tcggtttcgc ggaagttggc gatcacgtac cagtgacgag tgtcgattag
    19681 ggtaaaaata gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac
    19741 ccggccatca aacggtgcgc gaacggtcgc catttccaga tgcagtttgg tcagggcaat
    19801 atccgcttcg accgccgcac gctgggcaac taatgcatcc acgccgctga cggcgctggc
    19861 ggctgactgc gcctgcaaca atacagcatt aagatcggct tctgcagcgc gctgcgcggt
    19921 cctcgcacgg tcaacgtctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg
    19981 gcgtaatgta tctgtggcct gtttagcggc ggcacgggct ttttctaccg tggcgttaac
    20041 cgagtcggca ccaaactgtt gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc
    20101 cagtgccgcg agggaggctt cagatttcgc cagattggct tcgtacgggc gcgggtcaat
    20161 gcggaacagc aaatcgccct gtttgactgc ctggttgtcg gtgaccgcca gttctacaat
    20221 gcggccgctg acttccggca ccacatcaat ggtatctgct gacacgtaag cgtcattagt
    20281 tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg ccaacgcaac
    20341 cactaacaga gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc
    20401 gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc
    20461 ggtataaata attccgacaa atgccagctt gatatttgcc cgctgaataa ctcgcctcgt
    20521 gatgagcgtc aatataaggc tggcaatggc gcagaaaaac cagctgggat aataagcgcc
    20581 gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg gaattatcca
    20641 ggctgttttt ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc
    20701 ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc cattgaatgc gtcagtatgg
    20761 ctattttctt gatgaataaa aatagcttta ttcagccacc gcatt
//
LOCUS       DAZFKY010000074        20700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFKY010000074 DAZFKY010000000
VERSION     DAZFKY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..540
                     /gene="alaE"
                     /locus_tag="ACHKCL_002419"
     CDS             91..540
                     /gene="alaE"
                     /locus_tag="ACHKCL_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5763376.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(577..921)
                     /locus_tag="ACHKCL_002420"
     CDS             complement(577..921)
                     /locus_tag="ACHKCL_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5763377.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1073..1402
                     /locus_tag="ACHKCL_002421"
     CDS             1073..1402
                     /locus_tag="ACHKCL_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5763378.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1552..2886)
                     /locus_tag="ACHKCL_002422"
     CDS             complement(1552..2886)
                     /locus_tag="ACHKCL_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5763379.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            2974..3405
                     /locus_tag="ACHKCL_002423"
     CDS             2974..3405
                     /locus_tag="ACHKCL_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5763380.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3614..3859
                     /gene="nrdH"
                     /locus_tag="ACHKCL_002424"
     CDS             3614..3859
                     /gene="nrdH"
                     /locus_tag="ACHKCL_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5763381.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3856..4266
                     /gene="nrdI"
                     /locus_tag="ACHKCL_002425"
     CDS             3856..4266
                     /gene="nrdI"
                     /locus_tag="ACHKCL_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5763382.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4239..6383
                     /gene="nrdE"
                     /locus_tag="ACHKCL_002426"
     CDS             4239..6383
                     /gene="nrdE"
                     /locus_tag="ACHKCL_002426"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5763383.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6393..7352
                     /gene="nrdF"
                     /locus_tag="ACHKCL_002427"
     CDS             6393..7352
                     /gene="nrdF"
                     /locus_tag="ACHKCL_002427"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5763384.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            7708..8910
                     /gene="proV"
                     /locus_tag="ACHKCL_002428"
     CDS             7708..8910
                     /gene="proV"
                     /locus_tag="ACHKCL_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5763385.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8903..9967
                     /gene="proW"
                     /locus_tag="ACHKCL_002429"
     CDS             8903..9967
                     /gene="proW"
                     /locus_tag="ACHKCL_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5763386.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10024..11016
                     /gene="proX"
                     /locus_tag="ACHKCL_002430"
     CDS             10024..11016
                     /gene="proX"
                     /locus_tag="ACHKCL_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5763387.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11208..12392
                     /locus_tag="ACHKCL_002431"
     CDS             11208..12392
                     /locus_tag="ACHKCL_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763388.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12516..13253
                     /gene="ygaZ"
                     /locus_tag="ACHKCL_002432"
     CDS             12516..13253
                     /gene="ygaZ"
                     /locus_tag="ACHKCL_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5763389.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13243..13578
                     /gene="ygaH"
                     /locus_tag="ACHKCL_002433"
     CDS             13243..13578
                     /gene="ygaH"
                     /locus_tag="ACHKCL_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5763390.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13669..14199
                     /gene="emrR"
                     /locus_tag="ACHKCL_002434"
     CDS             13669..14199
                     /gene="emrR"
                     /locus_tag="ACHKCL_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5763391.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14326..15498
                     /gene="emrA"
                     /locus_tag="ACHKCL_002435"
     CDS             14326..15498
                     /gene="emrA"
                     /locus_tag="ACHKCL_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5763392.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15515..17053
                     /gene="emrB"
                     /locus_tag="ACHKCL_002436"
     CDS             15515..17053
                     /gene="emrB"
                     /locus_tag="ACHKCL_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5763393.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17117..17632)
                     /gene="luxS"
                     /locus_tag="ACHKCL_002437"
     CDS             complement(17117..17632)
                     /gene="luxS"
                     /locus_tag="ACHKCL_002437"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5763394.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17782..19338)
                     /gene="gshA"
                     /locus_tag="ACHKCL_002438"
     CDS             complement(17782..19338)
                     /gene="gshA"
                     /locus_tag="ACHKCL_002438"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5763395.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19411..19839)
                     /locus_tag="ACHKCL_002439"
     CDS             complement(19411..19839)
                     /locus_tag="ACHKCL_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5763396.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19836..20402)
                     /gene="yqaB"
                     /locus_tag="ACHKCL_002440"
     CDS             complement(19836..20402)
                     /gene="yqaB"
                     /locus_tag="ACHKCL_002440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5763397.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20432..>20700)
                     /locus_tag="ACHKCL_002441"
     CDS             complement(20432..>20700)
                     /locus_tag="ACHKCL_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763398.1"
                     /translation="PPGCTIFSEIFSNEDIEEVAELTAGTRKDNVALATARRANASES
                     SSRMHHLLQKISFKNISFLNNYFIHFLNFLLLRHQRQNQAITLK"
BASE COUNT         4908 a         5409 c         5259 g         5124 t
ORIGIN      
        1 tatacgctat gcgaaaatgc agatggcaat gagatccact gctttcatct ccattaacat
       61 cccattacgc ttttattaag gagcattagc atgttctcac cgcagtcacg cttgcgtcat
      121 gcagttgcag atacgttcgc gatggttgtt tactgttctg tcgtgaacat gtgtattgaa
      181 gttttcctct ccggaatgag cttcgaacag tctttttatt ccagattggt agcgattccg
      241 gtgaacatct taattgcatg gccatacggt atgtaccgcg atctgtttat gcgcgcggca
      301 cgcaaagtta gcccgtcggg ctggataaaa aatctggcgg atatcctggc ttatgtgacg
      361 ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg gcatcagatt
      421 atggcggcgg tcagttcaaa catcgttgtt tcgatgttga tgggggcggt ttatggctac
      481 ttcctcgatt attgccgccg actgtttaaa gtcagccgtt accagcaggt aaaagcctga
      541 ctcttctttt cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc caaataaacc
      601 attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac cgtaatgctc
      661 gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc
      721 ggaagcgggt tcagccagtg tcgaactgcg ttcttttaat gttggatgaa ttaccagagc
      781 ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac ggcggtaacc
      841 atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta cgcgcgccac
      901 ctcgtctggt cgtaaataca tatttgatcc tcgttattat caaccgcggg cctaccttac
      961 ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga acctcagtta
     1021 agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc atatgtttaa
     1081 ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg atgtcaatca
     1141 attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca aaggggaagc
     1201 tgaagccgca cgcagtaaag ctcaggcatt gctgaaagaa accagggcgc gaatgcatgg
     1261 tcgtactcgc gtccagcaag ccgcgcgcga tgccgtaggc tgcgcggatt cttttgttcg
     1321 tgaaagaccc tggtgtagtg tgggaactgc tgctgcggta ggtattttta tcggtgcgtt
     1381 gttgagcatg cgtaaatcgt agtgagacga tgacaagcag tagtctgact tctgaaaagt
     1441 actcagtgcg tcaacgcctt gtccgcctac aggcctgaaa aacatagcgc atcaggggat
     1501 tttgccctgg tcaccagtct tataggaccc cgaagcctgg cttcggggtt attaatttaa
     1561 agcactgcgc aattgctgtg ctacctgtcg cgccatactt tcgctaacga tattcgtaaa
     1621 cgagagcaat aatcctccgt cctcgcttga gcggatacgc cagtcgctaa gtgcctgtac
     1681 ggcaagacca gcagcattgg ctttatgcac gaatgtcacg tcattacctg ctagtttgat
     1741 caccatctgg ataccgcctt tttgcggcac aacctgaaat ccctgctgcg taagtgcctg
     1801 ctcaatccac tgccgtcgct gggcataatg ctggcgcatt ttcttcagat gccgccagaa
     1861 atgcccctca cggatgaaat ctgccagtgt gttctgccac agtacgggta cagcacatgg
     1921 ttccagtgac gcctgctgcc ggaattgcga aatctgctcc accggcacca ccagccacgc
     1981 acagcgcaat gcaggaaata gcgctttgct gaatgttccg gcataaatca cccgctgcgg
     2041 cgcgtcgaga ctttttagtg ccggtaacgg cttaccgtga tagcgaaact cactgtcgta
     2101 atcatcttca ataatccatg cctgactacg ctctgcccat tccagtatct gatacctacg
     2161 cgctaaagag agtgccacac ccagcgggct ttggtgtgcc ggagtaatca gggcaaaacg
     2221 cgcatcagga taattttgta ttccgcttgt gacatccagt ccgttgtcat caaccggcac
     2281 aggtaaaatt tccacaccgt ggcgagtgac aatcgggcga atcagcggaa agccgggatc
     2341 ttccatccac atcccgtctc ccggttgcgc cagagcgtgc agaattaaag ccattgaggc
     2401 cgcataaccg tgagtgataa agacctgctc tggctgacaa tcaatcccac gtgaaacgcg
     2461 taaataatca acaatagcct cgcgcaaggc cgcctcgccg cagacatcgc ctaatgccaa
     2521 atcaaagcgc gtctgggtac gcagacggcg gcccatcacc cgcgcccaca attcacgcgg
     2581 aaaaagatca agtgcaggta gccccatctg aaaaggcagc ggctgctgac tttcaccagc
     2641 aaaagccacg acatccacag cagactgttg cggatgtatc tgctcactga caaaggtacc
     2701 cgcctgtccc cgcctgacca gccatccctg cgccaccagc tcagcataag cattttcgac
     2761 agtggatcga gagacaccca gctcctgcga ccaggtacgg ctcgaaggta gccttgcgcc
     2821 aggtttcagt tttccttgct cgataaccgt ttttaactga cgggcgatat cctgataacg
     2881 tggcatatgg tctgcttttt taggtaattt atggctctct ttagcagtcc attatagtgc
     2941 tacattgcgg gcgttaataa gaggagaaac tgtatgacta cattacgtca gccttactac
     3001 gaacttagcc cggcagtgta taacgcactg gtgcaggcca aaacggcact ggaaaatagc
     3061 acgctggata ccacgttgat ggagttaatt tatttgcgcg tctcgcaaat caacggctgc
     3121 gcattttgtc tggagatgca cagcaaagca ttgcgcaaat ccggtgtgcc acaacacaaa
     3181 ctggacgccc tggcaggctg gcgcgtaagc catcattttg atgaacgcga gcgagcggcg
     3241 ctggcatggg cggaatctgt gaccgatatt gccagaaccc atgcggaaga cgaggtttat
     3301 cagcctttgc ttgagcattt cagcgcagca gaaatcagcg acttaacgtt tgccatcggg
     3361 ctgatgaatt gttttaaccg tctggccgtt tccatgcgga tgtaatttgc tgttgccggg
     3421 tatgcgcccg gcaaaaaaac acaaaatccc ctaccccgtt gagccattca ccggtggcgt
     3481 aatcaatcta aaattaccat atcttgtgtg gttgaatctt ttttcaacta catctagtat
     3541 ctctgtatca acagagagac aacccgacgc gtatcatcgc gccgtatctt cattttaaac
     3601 ggaaatacga atcatgcgca ttactattta cactcgtaac gattgcgttc agtgccacgc
     3661 caccaaacgg gcgatggaaa accggggctt tgattttgaa atgattaatg tcgatcgcgt
     3721 tcctgaagcg gcagaagcgt tgcgtgctca gggctttcgt cagttgccgg tagtgattgc
     3781 tggcgatctt agctggtctg gtttccgtcc ggacatgatt aaccgtctgc atccagcgcc
     3841 acacgcggcc agtgcatgag ccagctcgtc tacttctcca gcagctccga aaacacgcag
     3901 cgttttatcg aacgtttagg tctgcccgcg gtgcgcatcc cgctcaatga gcgggaacgg
     3961 attcaggtag acgagcctta catcctgatc gtgccctctt acggcggcgg cggtacggct
     4021 ggcgcggtgc cacgacaggt aattcgcttt ttaaacgacg agcacaaccg ggcgttgctt
     4081 cgcggcgtta ttgcttcggg taatcgcaac tttggtgagg cgtatggccg cgccggagat
     4141 gtgattgccc ggaaatgcgg cgtgccgtgg ctgtaccgtt ttgaactcat gggtacgcaa
     4201 agcgatatcg aaaacgttcg taaaggagta accgaatttt ggcaacgaca accgcagaat
     4261 gcctgacgca gaaaacgatg gattaccacg cgctgaatgc gatgcttaac ctctacgata
     4321 gcgcaggtcg cattcagttc gataaagacc gccaggccgt tgacgccttt atggcgacgc
     4381 atgtgcgtcc gaacagtgtg accttcagta gccagcagca gcgcctgaac tggctggtca
     4441 acgaaggtta ctatgatgaa agcgttctta atcgctactc tcgcgacttt gtcattacgc
     4501 tgtttgccca cgcacacacc agcggttttc gtttccagac attcctcggg gcatggaagt
     4561 tttacaccag ctatacgttg aagacattcg acggtaaacg ttatctggaa gattttgccg
     4621 atcgagtcac gatggtggcg ctgacgctgg cacaaggcga tgagacgctg gcgttgcaac
     4681 tgacagatga aatgctgtca ggacgctttc agccagccac gccaacattc ctcaactgcg
     4741 gtaagcagca gcgcggcgaa ctggtttcct gttttttgct gcgtattgaa gacaatatgg
     4801 agtcgattgg tcgggcggta aattccgcac tgcaactgtc gaaacgcggc ggcggcgtag
     4861 catttttgct gtcgaatctg cgggaagcgg gcgcgccaat taaacgtatt gaaaatcaat
     4921 cttctggcgt aattccggtg atgaaaatgc tggaagacgc attttcctat gccaaccaac
     4981 tcggcgctcg tcagggggct ggtgcagtct atttacatgc tcatcatccc gatattctgc
     5041 gttttctcga cacgaaacgg gaaaatgccg acgaaaaaat ccgcattaaa acactgtcgc
     5101 ttggcgtggt gatcccggat atcactttcc atctggcaaa agagaatgcg cagatggcgc
     5161 tgttttcgcc ttatgacgta gagcgagttt atggcaagcc gtttgccgat gtggccatca
     5221 gccaacacta tgacgaactg gttgccgatg aacgcattcg caaaaaatac ctcaacgccc
     5281 gtgatttctt ccagcgactg gcagaaatcc agtttgagtc cggctatccc tacatcatgt
     5341 atgaagacac ggtaaaccgt gctaaccctg tcgccgggcg cataaatatg agtaatctct
     5401 gctcagaaat tttgcaggtt aacagcgcct cagagtatga cgagaatctc gactatgccc
     5461 gcacaggcca tgatatctcc tgcaatttag gttcgttgaa tattgcgcac accatggatt
     5521 cccccgattt tgcccgcacg gtagagactg ccgtgcgcgg tttaacggca gtatcagata
     5581 tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa tgccgcctcg cacgccatcg
     5641 gactggggca gatgaattta cacggctatc tggcgcgaga aggcatcgct tatggttcgc
     5701 cggaagcact ggatttcacc aatctctatt tctatgccat cacctggcac gcactgcgta
     5761 cctcgatgtt gctggcacgc gaacgcggtg aaaccttcgc cgggttcaaa cagtcacgct
     5821 atgccagtgg tgaatatttt agccaatatc tgcaagggaa ctggcagccg aaaacgggga
     5881 aagttggcga actgtttgcc cgtagcggta ttacgttacc tacccgtgag atgtgggcgc
     5941 aactgcgcga cgacgtgatg cgctacggca tttacaacca gaatcttcag gcggtgccgc
     6001 caacaggttc tatctcttat atcaaccatg ctacgtcgag tattcatccg attgtggcga
     6061 aagtagagat acgcaaagag ggcaaaacag gacgcgttta ctaccctgcc ccgtttatga
     6121 ctaacgagaa tctggcgctg tatcaggacg cttacgaaat tggcgcagaa aagatcatcg
     6181 acacctacgc ggaagcgact cgccatgtcg atcaggggct gtcgctgacg ctgtttttcc
     6241 ccgataccgc caccactcgc gatatcaaca aagcgcagat ttacgcctgg cgcaaaggta
     6301 tcaaaacgct ctattacatc cggctgcgtc agatggcgct ggaaggcact gaaattgaag
     6361 gctgcgtctc ctgtgcactt taaggaatat ctatgaaact ctcacgtatc agcgccatca
     6421 actggaacaa gatatctgac gataaagatc tggaggtgtg gaatcgcctg accagcaatt
     6481 tctggctacc agaaaaggtg ccgctgtcga acgatattcc tgcctggcag acattaactg
     6541 tcgtagaaca acaactgacg atgcgcgttt ttactggcct gacgctgctc gacacgctgc
     6601 aaaatgttat cggcgcgcct tcgctgatgc ccgatgcact cacgcctcat gaagaagcgg
     6661 tattatcgaa tatcagcttt atggaagcgg ttcatgcccg ctcttacagt tcgattttct
     6721 cgacgctgtg ccagaccaaa gatgtcgatg ccgcctacgc ctggagtgaa gaaaacgcgc
     6781 cgttgcagcg aaaagcacag attattcagc aacattaccg cggtgatgat ccgctgaaaa
     6841 agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta ttccggtttc tggctgccga
     6901 tgtatttttc cagccgcgga aagctgacca ataccgcgga cctgatccgt ctgattatcc
     6961 gcgatgaagc agtccacggt tactacatag gctataaata tcagaaaaac atggaaaaga
     7021 tatctctggg acaacgtgaa gagttgaaga gtttcgcctt cgatttgttg ctggaactct
     7081 acgacaacga gttgcaatac accgatgagc tgtacgccga aaccccgtgg gctgacgatg
     7141 tgaaagcgtt tctctgttac aacgccaata aggctttgat gaatctgggc tacgaaccgt
     7201 tatttcccgc agaaatggcg gaagtgaatc cggcaatcct cgccgcgctt tcgccgaatg
     7261 ccgatgaaaa tcacgatttc ttttccggtt caggctcctc ttatgtgatg gggaaagcgg
     7321 ttgaaacaga atatgaagac tggaatttct gagggtgtta ttttcaaaaa tatcactacc
     7381 cgcgacaggg aaataattcc cgccaaatag ctttttatca cgcaaatatt ttgtggtgat
     7441 ctacactgat actctgttgc attattcgcc tgaaaccaca atattcaggc gttttttcgc
     7501 tatctttgac aaaaaatatc aacttttctc gatttgctct cagcccttat atcacgggaa
     7561 attccggcga tttgctcgca tcaatattca tgccacattt gccatcaggg gttgcctcag
     7621 attctcagta tgttagggta gaaaaaagtg actatttcca ttgggtaata tatcgacata
     7681 aacaaataac aggaatcttt ctattgcatg gcaattaaat tagaaattaa aaatctttat
     7741 aaaatatttg gtgagcatcc acagcgagcg ttcaaatata tcgaacaagg actttcaaaa
     7801 gaacaaattc tggaaaaaac tgggctatcg cttggcgtaa aagacgccag tctggccatt
     7861 gaagaaggcg agatatttgt catcatggga ttatccggct cgggtaaatc cacaatggta
     7921 cgccttctca atcgcctgat tgaacccacc cgcgggcaag tactgattga tggtgtggat
     7981 attgccaaaa tatccgacgc cgaactccgt gaggtgcgca gaaaaaagat tgcgatggtc
     8041 ttccagtcct ttgccttaat gccgcatatg accgtgctgg acaatactgc gtttggtatg
     8101 gaattggccg gaattaatgc cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag
     8161 gtcgggctgg aaaattatgc ccacagctac ccgaatgaac tctctggcgg gatgcgtcaa
     8221 cgtgtgggat tagcccgcgc gttagcgatt aatccggata ttttattaat ggatgaagcc
     8281 ttctcggcgc tcgatccatt aattcgcacc gagatgcagg atgagctggt aaaattacag
     8341 gcgaaacatc agcgcaccat tgtctttatt tcccacgatc tcgatgaagc catgcgtatt
     8401 ggcgaccgaa ttgccattat gcaaaatggt gaagtggtac aggtcggcac accggatgaa
     8461 attatcaata atccggcgaa tgattatgtc cgtaccttct tccgtggcgt tgatattagt
     8521 caggtattca gtgcgaaaga tattgcccgc cggacaccga atggcttaat tcgtaaaacc
     8581 cctggcttcg gcccacgttc ggcactgaaa ttattgcagg atgaagatcg tgaatatggc
     8641 tacgttatcg aacgcggtaa taagtttgtc ggcgcagtct ccatcgattc gcttaaaacc
     8701 gcgttaacgc agcagcaagg tcttgatgcg gcgctgattg atgcgccgtt agcagtcgat
     8761 gcacaaacgc ctcttagcga gttgctctct catgtcggac aggctccctg tgcggtgccc
     8821 gtggtcgacg aggaccaaca gtatgtcggc atcatttcga aaggaatgct gctgcgcgct
     8881 ttagatcgtg agggggtaaa taatggctga tcaaaataat ccgtgggata ccacgccagc
     8941 ggcggacagt gccgcgcaat ccgcagacgc ctggggtaca ccggcgactg caccgactga
     9001 cggcggtggt gctgactggc tgaccagtac gcctgcgcca aacgtcgagc attttaatat
     9061 tctcgatccg ttccataaaa cgctgatccc gctcgacagt tgggtcactg aagggatcga
     9121 ctgggtcgtt acccatttcc gtcccgtctt ccagggcgtg cgcgttccgg ttgattatat
     9181 cctcaacggt ttccagcaat tgctgctggg tatgcccgca ccggtggcga ttatcgtttt
     9241 cgctctcatc gcctggcaga tttccggggt cggaatgggc gtggcgacgc tggtttcgct
     9301 gattgccatc ggcgcaatcg gtgcctggtc gcaggcaatg gttactctgg cgctggtgtt
     9361 aaccgccctg ctgttctgta tcgtcatcgg tttgccgttg gggatctggc tggcgagaag
     9421 tccgcgagcg gcgaaaatta ttcgtccact gcttgatgcc atgcagacca cgccagcgtt
     9481 tgtttatctg gtgccaatcg tcatgctatt tggtatcggt aacgtgccgg gcgtggtggt
     9541 gacgatcatc tttgctctgc cgccgattat ccgtctgacc attctgggga ttaaccaggt
     9601 tccggcggat ctgattgaag cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt
     9661 caaagttcag ttaccgctgg cgatgccgac cattatggcg ggcgttaacc agacgctgat
     9721 gctggccctt tctatggtgg tcatcgcctc gatgattgcc gtcggcgggt tgggtcagat
     9781 ggtacttcgt ggtatcggtc gtctggatat ggggcttgcc accgttggcg gcgtcgggat
     9841 tgtgatcctc gccattatcc tcgatcgtct gacgcaggcc gttgggcgcg actcacgcag
     9901 tcgcggcaac cgtcgctggt acaccactgg ccctgtcggt ctgctgaccc gcccattcat
     9961 taagtaactc tgcacttgcc cggtgacgcc gggcatatca ccctgccaaa aaaaggaata
    10021 acaatgcgac atagcgtact ttttgcgaca gcgtttgcca cgcttatctc tacacaaact
    10081 tttgctgccg acctgccggg caaaggcatt actgttaatc cggttcagag caccatcacc
    10141 gaagaaactt tccagacgct gctggtcagc cgcgcgctgg agaaattagg ttataccgtc
    10201 aataaaccca gcgaagtgga ttacaacgtt ggctacacct cgcttgcttc cggcgatgca
    10261 accttcaccg ccgtgaactg gacgccactg catgacaaca tgtacgaagc tgccggtggc
    10321 gataagaaat tttatcgtga aggggtattt gttaacggcg cggcacaggg ttacctgatc
    10381 gataagaaaa ccgccgacca gtacaaaatc accaacatcg cacaactgaa agatccgaag
    10441 atcgccaaac tgttcgatac caacggcgac ggaaaagcgg atttaaccgg ttgtaaccct
    10501 ggctggggct gcgaaggtgc gatcaaccac cagcttgccg cgtatgaact gaccaatacc
    10561 gtgacgcata atcaggggaa ctacgcggcg atgatggccg acaccatcag tcgttacaaa
    10621 gagggcaaac cggtgtttta ctacacctgg acgccgtact gggtgagtaa tgagctgaag
    10681 ccaggcaaag atgtggtctg gttgcaggtg ccgttctctg cactgccggg cgataaaaat
    10741 gccgatacca aactgccgaa tggcgcgaat tacggcttcc cggtcagcac catgcatatc
    10801 gttgccaata aagcctgggc cgagaaaaac ccggcagcag cgaaactgtt tgccattatg
    10861 cagttgccag tggcagatat taacgcccag aacgccatta tgcatgacgg caaagcctca
    10921 gaaggcgata ttcagggcca cgttgatggc tggatcaaag cccaccagca acagttcgat
    10981 ggctgggtga atgaggcgtt ggcagcgcag aagtaatttt tattcgggcg gataaggcgt
    11041 ttacgccgca tccgccgttc tgtacacaat gcctgatgcg acgctggcgc gtcttatcat
    11101 gcctacaaac cacaccgcac atttcaacaa tctatctttc atcccatatt catcaacatc
    11161 cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg accaaaccta
    11221 atcatgagct tagcccggcg ctaatcgtgc tgatgtctat cgccaccggt ctggcggtcg
    11281 ccagcaacta ttacgcccag ccattgctcg acaccatcgc gcgtaacttt tccctttccg
    11341 ccagttcggc aggctttatt gttaccgccg cgcagttggg ctatgccgcc ggtctgctgt
    11401 ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg atgaccttac
    11461 tggcagcggg cggtatgtta atcactgcca gcagtcagtc actggcgatg atgatcctcg
    11521 gtacggcatt aaccggttta ttctcagtcg tggcacaaat tctggttccg ctggcagcga
    11581 cgctggcttc accggataaa cgcggcaaag tggttggcac cattatgagc ggtctgctgt
    11641 tggggatatt gctggcacga accgttgccg ggttactggc aaacctcggc ggctggcgca
    11701 ccgtcttttg ggttgcctcg gtgttaatgg ctctgatggc gctggcatta tggcgtggtc
    11761 tgccacaaat gaaatcagaa acccacctca actacccaca gttgttgggt tccgttttca
    11821 gcatgtttat cagcaataag atcctgcgca cccgcgcgtt gctgggctgc ctgacctttg
    11881 ccaacttcag cattctctgg acctcaatgg cctttttgct tgccgctcca ccttttaact
    11941 acagcgatgg tgtaattggt ctgtttgggc ttgcgggagc tgccggagcg ttgggcgctc
    12001 gtccggcggg cggttttgcc gacaagggca aatcgcacca caccacaact ttcggtctgc
    12061 ttctgctgtt actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga
    12121 ttatcggcat tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg
    12181 taatttatcg aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct
    12241 actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt
    12301 gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc
    12361 gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta
    12421 cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct
    12481 atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg
    12541 ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt
    12601 atatcccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg
    12661 aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga
    12721 tgctggcagc agggagtagt ttgtgggttg ctgcactgac cgtcatggca atggatgttc
    12781 gccatgtttt gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga
    12841 aaaccgccct atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac
    12901 tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat
    12961 ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag
    13021 gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc
    13081 tgctcgcctc ttttcagcgc aaacaatctc tttgcgttac cgcagcgtta gttggtgccc
    13141 ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct
    13201 gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg
    13261 ctgcttgggt tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg
    13321 cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt
    13381 ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat
    13441 acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa
    13501 acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa
    13561 gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt
    13621 tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg
    13681 tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat
    13741 caggagatcc ttctgactcg tctttgcatg catatgcaaa gcaagctgct ggagaaccgc
    13801 aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg
    13861 gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc
    13921 cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt
    13981 gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt
    14041 ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc
    14101 acaacagaaa aagaccagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag
    14161 atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat
    14221 ccagatttat aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa
    14281 gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg
    14341 gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc
    14401 cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta
    14461 ctgcgtcact tcgaagaaac cgatgacgca tacgtggcag ggaatcaaat tcaaattatg
    14521 tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa
    14581 ggcgacgtgc tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa
    14641 actgcactgg cttccagcgt tcgccaaacc caccagcaga tgattaacag caagcagttg
    14701 caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc
    14761 cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac
    14821 gccgttacca gtgcccaggc gcaactggac gtcgcgattc aacaatacaa tgccaatcag
    14881 gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa
    14941 gtacgtaacg cctggctggc gctggagcgt actcgtattg tcagtccgat gaccggttat
    15001 gtctcccgcc gcgcggtaca gcctggggcg caaattagcc caacgacgcc gctgatggcg
    15061 gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat
    15121 atgcgtatcg gtcagccggt cactatcacc accgatattt acggcgatga tgtgaaatac
    15181 accggtaaag tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg
    15241 caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tgcctgtgcg tatcgaactg
    15301 gaccagaaac agctggagca atacccgctg cgtatcggtt tatccacgct ggtgagcgtc
    15361 aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttccac tccggtagcg
    15421 gtaagcaccg cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta
    15481 aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg
    15541 cgcgcaactg gtcattatga cgattgcgct gtcactagcg acattcatgc aggtgctgga
    15601 ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatcttggct catcgctcag
    15661 ccagggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac
    15721 cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctctggtcaa ccatcgcctt
    15781 tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg
    15841 cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa
    15901 taattacccg ccagccaaac gctcaatcgc gctggcgttg tggtcgatga cggtgattgt
    15961 cgcgccaatt tgcggcccaa tcctcggcgg atatatcagc gataattacc actggggctg
    16021 gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaactct
    16081 gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct
    16141 ggttattggt atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactggtt
    16201 ttcatcacag gaaattatca tccttaccgt ggtggcggtg gtggctatct gcttcctgat
    16261 cgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa
    16321 cttcaccatc ggctgtttgt gtatcagcct cgcgtatatg ctctacttcg gcgcgattgt
    16381 tctgctgccg cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc
    16441 ctctgcgccg gtagggatta ttccagtgat cctgtcgccg attatcggcc gcttcgcgca
    16501 taaactggat atgcgccgac tggtaacctt cagctttatt atgtatgccg tctgcttcta
    16561 ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct ggccgcagtt
    16621 tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta cgctgtctgg
    16681 tttgccaccg gaacgactgg cggcggcatc gagcctctcc aactttacgc gtacgctggc
    16741 ggggtctatc ggcacgtcga taaccacaac catgtggacc aaccgcgagt cgatgcacca
    16801 tgcgcagttg actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca
    16861 actggaaggg cttgggatga cacaacagca ggcatcaggc tggattgccc agcagatcac
    16921 caatcagggg ctgattattt ccgccaatga gatcttctgg atgtcggccg ggatattcct
    16981 cgtcttgctg gggctggtgt ggtttgctaa accgcccttt ggcgcaggtg gcggcggagg
    17041 cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt
    17101 gaagatagtt tactgactaa atgtgcagtt cctgcaactt ctctttcggc agcgccagtt
    17161 cttcgttgct gttgatgcgc acgtcacgtt ccagaatgct acgcgcaata tcctgcgctt
    17221 cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagctcc ggaatctgat
    17281 tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccagacatca gcaacacgct
    17341 gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga
    17401 tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt
    17461 gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca
    17521 gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg
    17581 caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac
    17641 ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat
    17701 gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt ggccactcat
    17761 gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt
    17821 atctgcggct tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc
    17881 ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtacg cttcagcaaa
    17941 cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa
    18001 agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc
    18061 gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt
    18121 acccacctgc ggtaacggga actgtgcggt ttcgcagccg atacccagcg tcagacccgg
    18181 tttgcgacct tcgaggatca cccggttcca gttaacgcgt gtacaggcaa gttcgctact
    18241 gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac
    18301 ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat
    18361 atattcaatg ccgccacgta acagcgcatc agaaggcgac tcgccgctgc gggtaacgcg
    18421 ttttggacga atcggcgcgt acagttcgtt ttcaatctgc agcacgttgc tgttgatttg
    18481 cagcctctta ccgtctttat caataccaat cttcgcgtac tcttccgatg gcgttttgat
    18541 tgcctgtttc aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct
    18601 ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta
    18661 atacataccg cactcggttt tctcaaacgg cagcgacgtt ggtttacctt gcaggaatga
    18721 agagcatatt gccggagacg cgccaaacag ataaggaatg acccaaccga agcgatagta
    18781 gttgcgaata acgcggaaat agcccgcaga gattttctct ttagcatcag cgcccgaaat
    18841 atcgccacac ttcgcttgcc agaatgccat aggcaaagag aagttgtaat gcacgccgga
    18901 gatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacggtaca gcgttttaaa
    18961 gcgtccggtg ttagaagtgc cgtactgcgc cagttcgatg tcctgacctt ctgcgatgta
    19021 gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg
    19081 cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc
    19141 cagcaatgct tccgcaaaat cggtagtaat ccatttatgc gtcagtgcgg aacctaatgc
    19201 ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc
    19261 cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg
    19321 tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact
    19381 gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt
    19441 cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattaccgg
    19501 cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat
    19561 ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg
    19621 ccagcgcgat gttttacgca atggacagaa acgcccaagg ataacgttag ttaaccctcc
    19681 aaggctatta cccattgttg ctgttaaaac taaaacccag ggatgactga tcccagaaag
    19741 caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact
    19801 actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg
    19861 tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac
    19921 tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta
    19981 tggtgtttga cgtgatcggc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtga
    20041 gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga
    20101 ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata
    20161 cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc
    20221 agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc
    20281 tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta
    20341 ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac
    20401 atggtcttct cgcaagtcaa aaaatatgac gctactttag cgtaattgct tgattttgtc
    20461 gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga
    20521 tatttttgaa ggaaattttt tggagaagat ggtgcatccg ggaggatgac tcactggcgt
    20581 tcgcccttcg ggccgttgct aaagcaacgt tatccttcct ggtgcctgcg gttaattccg
    20641 ctacctcttc gatatcttca ttgctgaaga tttcggagaa tatggtgcat ccgggaggat
//
LOCUS       DAZFKY010000075        20540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFKY010000075 DAZFKY010000000
VERSION     DAZFKY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..162)
                     /locus_tag="ACHKCL_002442"
     CDS             complement(<1..162)
                     /locus_tag="ACHKCL_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763399.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRF"
     gene            complement(183..464)
                     /locus_tag="ACHKCL_002443"
     CDS             complement(183..464)
                     /locus_tag="ACHKCL_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763400.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1003..1089)
                     /locus_tag="ACHKCL_002444"
     tRNA            complement(1003..1089)
                     /locus_tag="ACHKCL_002444"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Leu,seq:taa)
     gene            complement(1102..1175)
                     /locus_tag="ACHKCL_002445"
     tRNA            complement(1102..1175)
                     /locus_tag="ACHKCL_002445"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1141..1143),aa:Cys,seq:gca)
     gene            complement(1229..1304)
                     /locus_tag="ACHKCL_002446"
     tRNA            complement(1229..1304)
                     /locus_tag="ACHKCL_002446"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Gly,seq:gcc)
     gene            complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHKCL_002447"
     CDS             complement(1456..2004)
                     /gene="pgsA"
                     /locus_tag="ACHKCL_002447"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5763401.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHKCL_002448"
     CDS             complement(2061..3893)
                     /gene="uvrC"
                     /locus_tag="ACHKCL_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5763402.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHKCL_002449"
     CDS             complement(3890..4546)
                     /gene="uvrY"
                     /locus_tag="ACHKCL_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5763403.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4688..4804
                     /locus_tag="ACHKCL_002450"
     CDS             4688..4804
                     /locus_tag="ACHKCL_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5763404.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            4842..5018
                     /gene="yecU"
                     /locus_tag="ACHKCL_002451"
     CDS             4842..5018
                     /gene="yecU"
                     /locus_tag="ACHKCL_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5763405.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5005..5229
                     /gene="yecF"
                     /locus_tag="ACHKCL_002452"
     CDS             5005..5229
                     /gene="yecF"
                     /locus_tag="ACHKCL_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5763406.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHKCL_002453"
     CDS             complement(5297..6019)
                     /gene="sdiA"
                     /locus_tag="ACHKCL_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5763407.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHKCL_002454"
     CDS             complement(6249..7001)
                     /gene="tcyN"
                     /locus_tag="ACHKCL_002454"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5763408.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHKCL_002455"
     CDS             complement(6998..7666)
                     /gene="tcyL"
                     /locus_tag="ACHKCL_002455"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5763409.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHKCL_002456"
     CDS             complement(7681..8667)
                     /gene="dcyD"
                     /locus_tag="ACHKCL_002456"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5763410.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHKCL_002457"
     CDS             complement(8772..9572)
                     /gene="tcyJ"
                     /locus_tag="ACHKCL_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5763411.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHKCL_002458"
     CDS             complement(9660..10211)
                     /gene="fliZ"
                     /locus_tag="ACHKCL_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5763412.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHKCL_002459"
     CDS             complement(10257..10976)
                     /gene="fliA"
                     /locus_tag="ACHKCL_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5763413.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11296..13053)
                     /locus_tag="ACHKCL_002460"
     CDS             complement(11296..13053)
                     /locus_tag="ACHKCL_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5763414.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            13319..14716
                     /gene="fliD"
                     /locus_tag="ACHKCL_002461"
     CDS             13319..14716
                     /gene="fliD"
                     /locus_tag="ACHKCL_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5763415.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            14741..15151
                     /gene="fliS"
                     /locus_tag="ACHKCL_002462"
     CDS             14741..15151
                     /gene="fliS"
                     /locus_tag="ACHKCL_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5763416.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            15151..15516
                     /gene="fliT"
                     /locus_tag="ACHKCL_002463"
     CDS             15151..15516
                     /gene="fliT"
                     /locus_tag="ACHKCL_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5763417.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            15594..17081
                     /gene="amyA"
                     /locus_tag="ACHKCL_002464"
     CDS             15594..17081
                     /gene="amyA"
                     /locus_tag="ACHKCL_002464"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5763418.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHKCL_002465"
     CDS             complement(17115..17528)
                     /gene="yedD"
                     /locus_tag="ACHKCL_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5763419.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            17715..18920
                     /gene="yedE"
                     /locus_tag="ACHKCL_002466"
     CDS             17715..18920
                     /gene="yedE"
                     /locus_tag="ACHKCL_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5763420.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            18917..19150
                     /gene="yedF"
                     /locus_tag="ACHKCL_002467"
     CDS             18917..19150
                     /gene="yedF"
                     /locus_tag="ACHKCL_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5763421.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            19259..19927
                     /gene="yedK"
                     /locus_tag="ACHKCL_002468"
     CDS             19259..19927
                     /gene="yedK"
                     /locus_tag="ACHKCL_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5763422.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            20038..20517
                     /gene="yedL"
                     /locus_tag="ACHKCL_002469"
     CDS             20038..20517
                     /gene="yedL"
                     /locus_tag="ACHKCL_002469"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5763423.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         5172 a         5252 c         4942 g         5174 t
ORIGIN      
        1 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacactt
       61 acattgttga tgaaatcgct cacgaaggac caccggacag gctttcagcc agaagcgcag
      121 attttcggga tgtgaaccgt taacggcaca taatgctcgc acaacaaacc tgttaaaaga
      181 gattactcag cattctcctg cttccattgc cggatcattt catcggtaac atcaccctca
      241 taacatacca cgtcataccc tccattacgg ctatatgcac tgcgtccacc acatctacta
      301 ccattccttg catgattata aggacacgca caattgcctg gataagattc aatggattct
      361 ttaattattt cttttttgat ctgagcatca gactttcctg ttgctgcata tccactaaaa
      421 gatactaaac aaaggcagat tgccattaat aaaccacatc gcataccgat acactcaatt
      481 actaaccaat catagcccac atcataaaat cgagatgagg agcacaatac ctttaacaag
      541 aaggtgtcat aacgataata tgtcgtgcaa ttcatcaaaa aaaagatcta tatcaaagag
      601 atagaactct tatcagtgct tcagacatcg gcattcgagg cagcagaaaa acagtaaatc
      661 tgacgaattt gccttttccc aagatgttgt cagattaaaa agatagatta gcgcaggtac
      721 ctgttggtgg attgcaggag gaaaaataaa gtcgagtgga aaaatcccag aaaaattttt
      781 gtttaacaga ggtcctggtt cactatgcga aatacggagg agctgcagaa gaattctgct
      841 tcatccttag agtttaaaca gcacgtgcac taccattgtt tctgcatatc gccacaccat
      901 cgccactcta ccgccattgc acaaaataca aatacgaaaa aaccacccga aggtggtttc
      961 acgacactgc ttattgcttt gattttattc ttagctttcc catggtaccc ggagcgggac
     1021 ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta ccgattccac
     1081 catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac tagacggatt
     1141 tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct ttcagccaga
     1201 catccgctga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga actcgcgacc
     1261 ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat caagcaatca
     1321 gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat gcgttgcatt
     1381 ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg tttgctgaaa
     1441 attgcgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca aatattgcaa
     1501 cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccgg cgtactcaac
     1561 ccaaatgttc ggacgccaca gcagccatgc caacgccacc atctgggcag tggttttcac
     1621 tttcccaatc caggagacag ccacgctact gcgtttaccc aactccgcca tccattcgcg
     1681 tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaagg tcacccacca
     1741 gctgtggtaa tgctcggtta ccagcaccat ggcgatagcc acgagaactt tatctgctac
     1801 agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca gaaaaccatc
     1861 gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg gcgaccaagt
     1921 gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga acagtgtaag
     1981 caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag taagattacc
     2041 cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc tgccagacct
     2101 tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa accttgcaaa
     2161 ccgcccatat atttcaacaa catttgccgg cgttttggcc cgacgccttc aatggtttcc
     2221 agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat cgcgtgatcg
     2281 tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gagaatctgg cggcaaactg
     2341 aatccctcac cttccggctc aaagaacagc gtttccagcc cagccttacg atctgctcct
     2401 ttggcaacac caagtagcag cggatgattt ttatcccatg agacatccag ttcggcgaag
     2461 acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taagtatcac atccgggatc
     2521 ttactgtcgt cgatggcttt accataacgc cgacgcagca cctgattcat cgccgcataa
     2581 tcatcgcccg gcgtgatgcc agtaatgtta tagcgccgat actccgcacg cagcgggccg
     2641 ttagcatcaa acaccacaca ggaagcgacg gtttgttcac ccatggtatg gctgatgtca
     2701 aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagtgc tgtaagccgc
     2761 tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc attggtgcgc
     2821 gcgagtttca ggtaacgcgc cctatcgcca cgaggtttgg tttgaacatt aatcttgcgt
     2881 cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt aaaatcgagc
     2941 aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg gcctacgaag
     3001 gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata gctgcggctg
     3061 ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatacc cgcatcgaac
     3121 gccacaccaa taacgtcgag gtcgtcgccg gtattggaaa cgaattgttt ttcggtgacg
     3181 cgtcgcaccg cctgaatttg gtcgcgaata cgtgccgctt cttcaaactc cagattctga
     3241 ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt gccagacaaa
     3301 aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag tccttcaacg
     3361 cacggtccca gacagcgccc tatctgatat tgcagacacg gacgcgagcg attgcgataa
     3421 acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag tgtttcacgt
     3481 acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt tgcaccacga
     3541 tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata tgatttatca
     3601 tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg ttccagcaac
     3661 agcgcttcgg tttctgtatg agtaaccgtt acatcaattt gctggatctg ggcgaccagc
     3721 gcttcggttt tgcgcgaagc gaggttgcta cggaaatagc tggaaagccg ttttttcagg
     3781 tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg ataaacgcca
     3841 ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact cactgacttg
     3901 ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc tcaacatcgc
     3961 catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt ttcggactga
     4021 gattgagctg ttctgagatc tcattgacct tctggccctt cgtgatcatc agcataatct
     4081 gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt tcgatctggc
     4141 ttaatgccat ttgttgagcg atgtcagaag caatgtaacg ctgcccagaa tagacagaac
     4201 ggatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagccc gcagcaccgg
     4261 cctgcatgac tttcgctggt aaagggtttt ctgtatggac ggtaagcatg atgattttta
     4321 catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg cccggcatac
     4381 tcatgtccat tagcaccacg tcaacggcat ttgcccggca ccacttaacg gcgtcttcac
     4441 cgcacgatgc ctcaccgacg acttttatac cctttatatc ttccagaatg cgtcgtatcc
     4501 ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga atatctccag
     4561 aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt tattgcaact
     4621 ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa ttcgattgtc
     4681 cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag taagccctgc
     4741 gacgaaatgt gacaaaaatt acctttattc agcaaaaatg aaaatcagcc cgctgaatat
     4801 gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa atatttattg
     4861 gtcattatat taacgcattt aatagcaccg ttgacggcac gctcaaaaaa caaccactgc
     4921 tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc gcaaattttc
     4981 attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc cgagaataat
     5041 caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat gctgcaggcg
     5101 atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aacaacagct tgcgctgatc
     5161 gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa acaagcctac
     5221 cgtcagtaat ataaaaccgg ctgatagcgt gcctttcagc cggtttttgc atctggcacg
     5281 caggacagaa aagagatcaa attaagccag tagcggccgc gtaacaggca acctgggtct
     5341 tatttggtgc attaattttt ttctgcatgt ttttctggtg gaaattgacc gtattctcag
     5401 agattgacaa aatcatcgct atctctgctg atgtcttccc ttccgccgtc cacctcagaa
     5461 tttctttttc gcgcttgctg aaattcatct ctggcgtcat cactatttca tcatttaaac
     5521 gcatcagagc catcagactt tcgcgcacca gtaactgcat ttttaattgc agttcatcac
     5581 taagaatggg tatttcgcgc gcgctgcaac gggaaaagga caaaaagccc agcgcccggt
     5641 ttggcagcat taaatactga gtgacaccgc ggcgtaaacc atgtgcgcgc gcggcttccc
     5701 ataacggctg tgcttcgttg aataagtcat cattccacat tagatggccc tgactaaagt
     5761 tttcagggtt cagcaccgga tcaattgcga gaaagttttt tgcctgataa taactaaccc
     5821 acgcctcagg gtaattggtg tagaaagcca ctttaggtcg agtgaatggt accgggtggc
     5881 ggacacataa cgaatagtaa tcgtactcca gctgctgagc ctgaagctca atttcatggt
     5941 agacctcttc tgcggtctcc atcctctgaa aacgcaacag catcgtgcga cgccagctga
     6001 aaaaatccgt atcctgcata gtaaaccgca acgcccctga gagtgagtat catttataat
     6061 gataattcaa actatcacat aaataagatt tatatataat ttatattatt caggcaatga
     6121 attacttttg caagccatcg cattctctta tgttattaat gagttatgct gattttttaa
     6181 gcagttttat caggcttgaa atggcgttcc gccccgactg tcgaatcgtc ggggctgatt
     6241 ttttcttatt attgcagcag aaacttctcg aggaactggc gggtgcgagg ctgctcggga
     6301 tcggcaaata acgcttttgc ggccccctgc tcgactatcc gcccctggtc cataaaaatc
     6361 gcccggtccg caacatcccg ggcaaagctc atttcgtgtg tcacaatcac catcgtgcgc
     6421 ttttcctgcg ccagctgacg gatggtgttc aggacttcgc ccaccagctc tggatccagc
     6481 gctgacgttg gctcgtcaaa caaaatcacc tcaggacgca ttgccagcgc acgcgcaatc
     6541 gcaacacgct gctgttgacc gccagacaaa cgacgtggat agctggtttc tttaccggcc
     6601 agcccaactt tcgccaacag cttgcgagcg cgcgccgtgg cctcttcttt cggttcgcct
     6661 ttgacgatca ccggcccttc aataatgttc tccagcaccg tacgatgcgg aaacaaatta
     6721 aagttctgga agacaaaccc gacgtgctga cgcaactggc gaatcagcga tttttgctga
     6781 cttaatgaac gtgcagtatc aatagtgata tcgccaacgg tgatcgttcc cgcttccggt
     6841 tgttccagca gatttatgct acgtagcaat gtggttttgc cggaaccact cggaccgata
     6901 attgccacca cttcgccagg ctttacctcg aggtcgatac cgtgcagcac cgtctgaccg
     6961 tggaattttt tcactaggtt cttaacttca atggcactca tttgggttct ctctcctggc
     7021 gattaagttg attctcaaaa tggttctgca acgtcgataa cacagtcgcc atgatccagt
     7081 agatgagcga agccgccaga tacatggtga atacctccag cgtacgcgag gtaatcaact
     7141 gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc cagcgacgta tcttttacca
     7201 gactgataaa gctgttcgag agcggcggca acgccacccg cgccgcctgt ggcaaaatcg
     7261 cgcgacgcat agtctgccat ggcgtcatac caatactggc agctgcttcc cactgacctt
     7321 tatcaataga agaaatggcg gcgcgaagcg tttcggcagt ataggcggca gtgttgagcg
     7381 acagaccaat cattgctgac ggaatgggat ctaattcgat accaaactgc ggcaggccgt
     7441 aatagatcat aaacagttgt gcgatgagcg gcgtaccacg aaaaatagag atataaaaac
     7501 gcgccagcca gcgcacgggc cagattggcg acaggcgcat cagcgcgaga ataaacccta
     7561 gcagtaaacc aaaaaacatg ccgccgatac tgagttgcag cgtataccca gcccctttca
     7621 gcaggaacgg caaggaatca ataaccagtt gtatactttc ttgcatgagc gttcccggag
     7681 ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg ccggtatgaa taaacagaat
     7741 cggcccttca tctttgaagc gtttctgact gataccgtca atcagacccg ccatcgcttt
     7801 tccggtatac acaggatcaa gcagaatgcc ttccagccgc gccagcaatt tcactgcttc
     7861 catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa tagtcatccc agagtaaaat
     7921 ttccactgat gcggtcagct ccagttcttt cgcaatcgcc tgttgtaggt taaccacttt
     7981 cggcaattga tcggcaacgg aacgcgacac ggtcacgcca atcagttcgc tttcaggcat
     8041 caggtgttcc agcccaacag ccagtccggc gtgagttccg gcactgcccg atgcgactac
     8101 caccgatgaa atattaaccg ccccttcaca ctgttgcgcg atttccagcg cactctccac
     8161 ataacctagc gcgccaagag cattagaacc gccaaccgga atgacatacg ggcgaaagcc
     8221 ttgtgcttcg actcgcgtcg ccagctcttc cagttgggca ttgggatcgg tcagtgcgtc
     8281 gcacatttca atctgggtat tgaacagatc cagcaacaag cgattgccgt tggttaaata
     8341 gttttctgcg gttgtgccaa taggattttc cagcagcgcc acgcagtgca gaccgagttt
     8401 ggcagcgact gcggcagtct gccgcacatg gttagactgg atcgccccgg cagtaatcag
     8461 cgtatcggca ccttcacgca gagcatctgc cgcgagaaat tctagcttac gtaatttatt
     8521 gccgcccatt gccatggggg tgacgtcatc ccgtttgatg aaaatttccc gtcctagata
     8581 atcagaaaag cgcggcagat attcgagcgg cgttggcgcg ccgataaact ccagccgtgg
     8641 aaaacgggtt aaattatgca gtggcataac agcctccgat gtgtgttgtt gtgattttct
     8701 cattatgcac gctgaaaacg cgtaaataaa aaaggcgcta gtgaaagcgc ccttttttgt
     8761 cattatgctg attatttggt cacatcagca ccaaaccatt tttcggaaag ggcttgcaaa
     8821 gtgccgtctt tttgcatttc cgcaattgca tcattcactg ctttcagcag gtcttcattt
     8881 cctttacgca gcgccacgcc agactcctga cgggagaatg cttcaccggt tactgccagc
     8941 gtatcgttgg ttttcttcac cagatccagc gccgccagac gatcaacgag gatcgcatcg
     9001 atacgcccta cgcgcagatc ctgatatttg gtcgggtcat catcataggt acgcacatcg
     9061 acgccctgaa cattctgccg cagccactct tcatagttgg tgcccagacc gacccccact
     9121 tttttgcctt tcagatcatc ggctgtttta atggtgcctt cgttaccttt tttcaccagc
     9181 gcctgaatac cagaaatggt gtacggggtt gagaaatcgt attttttctt gcgctcatca
     9241 gaaatggtga cctgattaat caccacatca atacgtttag agtccagcga cgccagcata
     9301 ccgtcccatt tggtcggttt tagtgacgcc tcaacgccaa gatgttttgc cagctgttgg
     9361 gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc cctgaaaact gaacggcgga
     9421 taagtacctt ccagccctac cagcagcgtg ccgcgctctt taactttatt aagcagacct
     9481 tcatctgcaa aacttttaac gctcatgccc gcaatcagcg ctacggccat cacacccatc
     9541 aatgcctgac gtcccagatg tgctaatttc atattcaccc cgaatgttgt tatgtctgtt
     9601 tgcagtgtag agccattgtt tgtaaacaca aaaacaactc cgctacatct tattcttatt
     9661 taatatatat cagaagcagg caggctggag gatttctgca caagtccggt acaggtttgg
     9721 cgctgatagt gttgatactt ccgtaacgcg atgcggtaat tgttggtgct ggtggtgggc
     9781 agccacgggg cgaggatttc atcaaggaaa ccgtcctgca gctgatcgag ggaaatattt
     9841 tgctcgtgta gatgattccc caaacggcgc agacgaacga catattcacg caccgtacca
     9901 gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc gaaaaaatca
     9961 ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca atgccgccca tgatttttgt
    10021 tccgtttgcc agccattttc atcaagcagc gtgtccaggc gggaaatctc gattttattc
    10081 actattttgc cgtcgcgaac taaggtaatg cgatcgagca atttacggca atgcgcgcaa
    10141 tgggtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct tttcaggtgc
    10201 tgcaccatca ttaagaactc ctggtagtca aagttaaagt gcggcattta ctgacgttat
    10261 aacttaccca gtttagtgcg taaccgttta atagcctggc tgtgtaactg actgacccgc
    10321 gattccccga cctccagcac tgcaccaatc tctttgagat tcagctcttc ctggtaatag
    10381 agggttaata ccagtttttc gcgctccggc aacgtttcga tagcttccat cacccgctgg
    10441 cgcagattac tgtccagtag ttgttgtagc gggttttctc gttgatgatc atcagtaacc
    10501 aattcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag ctggctgtta
    10561 ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg ttccgctacc
    10621 tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc ctgtgccact
    10681 tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag ttcatccagc
    10741 atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg tagggcgtca
    10801 tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc gtcaagttcc
    10861 acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag cgggacataa
    10921 cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga attcacgata
    10981 aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg gtttttaatc
    11041 ggacgattag tgggtgaaat gaggggttat ttgggggtta caggtaaatt ccaggcagaa
    11101 aaaaccccgc cggtggcggg gaagcacgtt gctaacaaat tgcgctttat gttgccggat
    11161 gcggcgtaaa cgccttatcc ggcctacaag aatgtgcaaa ttcaataaat tgcaattcaa
    11221 cttgtaggcc tgataagcgc agcgcatcag gcaatttggc gttgccgtca gtctcagtta
    11281 atcaggttac aacgattaac cctgcagcag agacagaacc tgctgcggta cctggttagc
    11341 ttttgccagc acggagttac cggcctgctg aatgatctgc gctttcgaca tgttggacac
    11401 ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct tcggacaggt tggtagtggt
    11461 gttgttcagg ttggtgactg cggaatccag acggttctgg atagcaccca gggaagaacg
    11521 gaatttgtcg atggagctga ttgcgtcgtc cagggcagca agcgggttgg tcgttgcaga
    11581 accagcactg gtagtctcag tagtgatttt acccgcggag ttcacattta cagcagtacc
    11641 aattgctgga gcataatctt tattggtatc agtcgctgaa gcatacccgg caacagtcac
    11701 agagccgtta tccttgttaa cgctgtaaga gactgtatag tcggcaacgt tagtgatacc
    11761 acctttatca tccacataag acttcgcagc atcactggat tcacccgcgg taaacccaat
    11821 agttttgctc agtacaccgg aattaaaggt aattgaagat gtcgcggcag ccgctttggt
    11881 ttcgctcaat actacatctt tactcactgt agcctgataa gtaatcccac cagggattaa
    11941 cggagctact ggagtggttg tcgcaggagt tgcttttgcg atggtatatt ctgtgccatt
    12001 gaatgtcaga gaggcaccgt cactaccttc gctcgctgct ttgagcagcg ctttcatatc
    12061 agctttagct gcgctaccag cgttgttagt cgtcaagttg cctgcatcgt ctacgtatgc
    12121 ctggcttcca ccgatggtga tattacctgc tgaatccgtt tcgaaagaaa cagtaccatc
    12181 ttttgtggtg taagaaccat taacagattt acccacatca gcatgaaggg tggcagctaa
    12241 cgcatcacgt tttgaagctg aatcagcagc ctgcgccgcg gcgtctacac ccgtaccagc
    12301 agtagattta gtggtagtaa aatcaccaga tttagcgttg taagtataat ctacgccgcc
    12361 aactgtgact ttatcgccat tccctaattt atcgaatgca gcatcggtag ttaacaaggt
    12421 attttcggtt ttcagatcat aaagacctgt cgtggtgttt aacttcgcgc cagcagaagt
    12481 taaatcactt accgttgcag ctttgttggt aatagtacct ttaccattta cgttaaagcc
    12541 attcagaccc agagtatcag aatcgatttt tttcaggtcg atcgtgatgg tttcgccgtc
    12601 attcgcacca acctgaattt tcattgaacc gtctttcgcc agcacgttca cgccgttgaa
    12661 ctgggtctgg ccggatacgc ggtcaatttc atcaagacga gatttgattt cgtcctggat
    12721 ggagtccagg tcagaatcgg agttagtccc tgtagtggcc tgaaccgtca gttcacgaat
    12781 acgctgtaag ttgttgttga tttcggacag cgcgccttcg gtggtctgcg caacagaaat
    12841 accgtcgttg gcgttacggg ccgcctgagt caggccttta atgttagaag taaaacggtt
    12901 agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag
    12961 acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag
    13021 cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt
    13081 tgattacgta ttgggtttcc acccgtcggc tcaatcgccg tcaaacctgt tatcgtctgt
    13141 cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt cgccgaaata
    13201 ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc
    13261 aattcaggcc gataaccccg gtattcgttt tacgtgtcga atgataaaag gaaatcgcat
    13321 ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa tccttgatag
    13381 cctcaccgcc gcgcaaaaag cgacgctaac ccccatttca aatcagcaat cgtcgtttac
    13441 cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa
    13501 tactgcattg tctaaagccg atcttttttc cgccaccagc agcaccaccg cgttcagtgc
    13561 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    13621 ggcgcaaacc ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag
    13681 cgacagtaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    13741 cgcggctaac tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcaggtgt
    13801 tagcgcgagc attattaacg tgggtaacgg tgattatcgt ctgtcagtca catcaaatga
    13861 caccggcctt gacaatgcga tgacgctctc ggttagcggt gatgatgcgc tacaaagttt
    13921 tatgggctat gacgccagtg ccagcagcaa cggcatggag gtctcggtcg ccgcccagaa
    13981 tgcgcagctg acggtcaaca acgtcgccat tgagaacagc agcaacacca tcagcgacgc
    14041 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    14101 cactcaggac acctccaaag cgcaaacggc gattaaagat tgggtgaatg cctataactc
    14161 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    14221 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    14281 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgt tggcgcagat
    14341 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    14401 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    14461 cggcatcacg accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    14521 taaagccgct accgatggcg ttagtaagac cctgaataaa ttaactaaag actacaacgc
    14581 cgccagcgat cgcattgacg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    14641 cgttttaatg acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa
    14701 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    14761 ccaagcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct
    14821 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca
    14881 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    14941 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ctaacccaaa acttgattgc
    15001 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    15061 agtcgaagaa gtggaaacat taatgcgcaa tattgccgat gcctggaaag agtcgttact
    15121 ctccccttct ttgattcagg acccagtctg atgaaccatg caccgcattt atatttcgcc
    15181 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    15241 gacgaactca tcgccagcga aatggcgtat gttaatgcgg tgcaggagat tgcacatttg
    15301 actgaagagg ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgcgcctg
    15361 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    15421 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttga gtgcctatgg cgatcagggc
    15481 ggctttgtgc tggctccgca ggataacctc ttttgaatct gaatgagtcg atggctcgcg
    15541 aataatccga ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta
    15601 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    15661 aattggccga gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc
    15721 ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg
    15781 atttaggtga gtttgatcag aaaggcagca tccctactaa atatggcgat aaagtacaac
    15841 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgttg gacgtggtag
    15901 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgc gttaatgctg
    15961 atgaccgtac gcaaattgat gaagaaatca ttgaatgcga aggctggacg cgttacacct
    16021 tccccgctcg tgccgggcaa tactcacagt ttatctggga tttcaaatgt tttagcggta
    16081 tcgaccatat cgaaaacccc aatgaagatg gcatttttaa aattgttaac gactacaccg
    16141 gcgaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggcg
    16201 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    16261 tgatggaaca aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccagcct
    16321 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    16381 tggcggagta ctggtcgcat gaagttgata agctgcaaac gtatattgat caggtggaag
    16441 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    16501 gacgcaacta cgacatgacg cagattttca ctgggacgct ggtggaagcc gatccttttc
    16561 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    16621 tcgaaccgtg gtttaaaccg ctggcgtatg ctctaatttt gttgcgggaa aatggcgttc
    16681 cttcggtgtt ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc
    16741 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    16801 agcgtttcgc ccacggtgta cagacgttat ttttcgacca tccgaactgc attgccttta
    16861 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    16921 gcgaaaaaac cattcattta ggagagaatt acggcaataa aacctggcgt gattttcttg
    16981 ggaaccggca agagagtgta gtaaccgacg aaaacggcga agcgaccttc ttttgcaacg
    17041 gcggcagcgt tagcgtgtgg gttatcgaag aggtgcttta aattcatccc cggcggcaag
    17101 ccggggagat ttcattacgg caatggcgtc ggtaacggcg ctttatccag cgcagcggca
    17161 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    17221 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    17281 atcaatgtca acttgcccgg cacagcaatc acccgctgcc actgacggca atctagggta
    17341 tcgccctctt tagtgacaat caggctgcct atcgcttccg ggctgaccag cgcacgttgc
    17401 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    17461 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    17521 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
    17581 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
    17641 ccccgaaaat aaatttgcat ggtgtatgat tcgcgactct tgtttcaaac cttctgagtt
    17701 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
    17761 cgcccatccc cgcggtcatc gcggcgggta ttctctccac ttactatttt ggcatcactg
    17821 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
    17881 tcggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
    17941 taacccgcat cgacgggatg atgatcctcg gtatgtttgg cggctgcttt gccgcagcgc
    18001 tgtgggccaa caatgtcaaa ctacgcatgc cgcgtagccg tatccgcatt atgcaggcca
    18061 ttattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
    18121 cattctttac cggtattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
    18181 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
    18241 aaatgcagaa agttgctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
    18301 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacagcga
    18361 tgaaccaacc caaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
    18421 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
    18481 atatggcgaa agcaatcatt attggtatgg cggtgagtgc catcgggatc ttcagttacg
    18541 tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt
    18601 tactgtttgg ttttggcatc gtgctggctg gcggctgcga aaccggctgg atgtaccgcg
    18661 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caatgtgatc ggctcaacga
    18721 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
    18781 acttgctgaa aacctttggt ccgatggggg gcctgctggt gacatatttg ctgttgtttg
    18841 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
    18901 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
    18961 accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcagttaa aaaaagggga
    19021 aatcctggaa gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg
    19081 taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc gttatttaat
    19141 tcagaagtaa tccctcattc ccgctgtgac ccacagcggg aacatcgtta cctttgtatc
    19201 acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg aggtttctat
    19261 gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga
    19321 tattgaacgc gatattccct atgatcccga acccattggc agatacaacg tcgcgccggg
    19381 aaccaaagtc ctactgctca gtgaacgtga tgaacacctt catctggatc cggttttctg
    19441 gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg tagaaactgc
    19501 ggcaaccagt cgtatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga
    19561 tggctggttt gagtggaaaa aagaaggcga caaaaagcag ccttatttta tctatcgtgc
    19621 tgatggacaa cctgttttta tggcagcgat aggcagcaca ccgttcgagc gcggtgacga
    19681 agccgaagga tttttgattg tcactgcagc agcagaccag ggtctggtag atattcatga
    19741 ccgccgcccg ctggttctgt cgccagaagc tgcgcgggaa tggatgcggc aagagattag
    19801 cggtaaagaa gcctcagaaa tcgcggccag tggctgtgtt ccagcaaacc agttctcctg
    19861 gcacccggta tcgcgcgcgg tgggtaatgt taaaaaccag ggtgcggagt taattcaacc
    19921 tgtttgataa gcctggagat tattgatcaa caatactgcg tcataagtaa tctctattag
    19981 acaaagattt cattacctgt tggcatattg caaaaataac accaatacgg aatcgtcatg
    20041 ttcacgatta aaacagatga tctcacccat ccagcagtgc aagcattagt ggcttaccat
    20101 atttccggca tgctgcagca gtctccccct gaaagcagtc atgctttaga cgtgcaaaaa
    20161 ttacgtaacc cgacagtgac attctggtca gtatgggaag gcgaacaact cgcaggaatt
    20221 ggtgcgctga agttgctgga tgataaacat ggcgaactga aatcaatgcg gaccgcgcca
    20281 aattatttac gtcggggtgt cgccagtctg attttacgcc acattttgca ggtcgcccat
    20341 gacagatgcc ttcatcgcct gagtttagaa acgggtacac aggctggatt tacggcctgc
    20401 catcaacttt atttgaagca tggtttcgtt gattgcgaac cgtttgccga ttatcaactt
    20461 gatccacaca gtcgattttt gtcattgacg ctatgcgaag ataatgagtt gctttgagcc
    20521 agacgcagca cattcttgca
//
LOCUS       DAZFKY010000076        20356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFKY010000076 DAZFKY010000000
VERSION     DAZFKY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(129..875)
                     /gene="cutC"
                     /locus_tag="ACHKCL_002470"
     CDS             complement(129..875)
                     /gene="cutC"
                     /locus_tag="ACHKCL_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5763424.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(889..1455)
                     /gene="yecM"
                     /locus_tag="ACHKCL_002471"
     CDS             complement(889..1455)
                     /gene="yecM"
                     /locus_tag="ACHKCL_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5763425.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1671..3404
                     /gene="argS"
                     /locus_tag="ACHKCL_002472"
     CDS             1671..3404
                     /gene="argS"
                     /locus_tag="ACHKCL_002472"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5763426.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3446..3490)
                     /locus_tag="ACHKCL_002473"
     CDS             complement(3446..3490)
                     /locus_tag="ACHKCL_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763427.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3581..4069
                     /gene="yecT"
                     /locus_tag="ACHKCL_002474"
     CDS             3581..4069
                     /gene="yecT"
                     /locus_tag="ACHKCL_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO5763428.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4189..4581)
                     /gene="flhe"
                     /locus_tag="ACHKCL_002475"
     CDS             complement(4189..4581)
                     /gene="flhe"
                     /locus_tag="ACHKCL_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5763429.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4581..6659)
                     /gene="flhA"
                     /locus_tag="ACHKCL_002476"
     CDS             complement(4581..6659)
                     /gene="flhA"
                     /locus_tag="ACHKCL_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5763430.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6652..7800)
                     /gene="flhB"
                     /locus_tag="ACHKCL_002477"
     CDS             complement(6652..7800)
                     /gene="flhB"
                     /locus_tag="ACHKCL_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5763431.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8002..8646)
                     /gene="cheZ"
                     /locus_tag="ACHKCL_002478"
     CDS             complement(8002..8646)
                     /gene="cheZ"
                     /locus_tag="ACHKCL_002478"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5763432.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8657..9046)
                     /gene="cheY"
                     /locus_tag="ACHKCL_002479"
     CDS             complement(8657..9046)
                     /gene="cheY"
                     /locus_tag="ACHKCL_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5763433.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9061..10110)
                     /gene="cheB"
                     /locus_tag="ACHKCL_002480"
     CDS             complement(9061..10110)
                     /gene="cheB"
                     /locus_tag="ACHKCL_002480"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5763434.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10113..10973)
                     /gene="cheR"
                     /locus_tag="ACHKCL_002481"
     CDS             complement(10113..10973)
                     /gene="cheR"
                     /locus_tag="ACHKCL_002481"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5763435.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10992..12593)
                     /gene="tap"
                     /locus_tag="ACHKCL_002482"
     CDS             complement(10992..12593)
                     /gene="tap"
                     /locus_tag="ACHKCL_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5763436.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNTSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12639..14300)
                     /gene="tar"
                     /locus_tag="ACHKCL_002483"
     CDS             complement(12639..14300)
                     /gene="tar"
                     /locus_tag="ACHKCL_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5763437.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14443..14946)
                     /gene="cheW"
                     /locus_tag="ACHKCL_002484"
     CDS             complement(14443..14946)
                     /gene="cheW"
                     /locus_tag="ACHKCL_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5763438.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14967..16931)
                     /gene="cheA"
                     /locus_tag="ACHKCL_002485"
     CDS             complement(14967..16931)
                     /gene="cheA"
                     /locus_tag="ACHKCL_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5763439.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16936..17862)
                     /gene="motB"
                     /locus_tag="ACHKCL_002486"
     CDS             complement(16936..17862)
                     /gene="motB"
                     /locus_tag="ACHKCL_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5763440.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17859..18746)
                     /gene="motA"
                     /locus_tag="ACHKCL_002487"
     CDS             complement(17859..18746)
                     /gene="motA"
                     /locus_tag="ACHKCL_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5763441.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18873..19451)
                     /gene="flhC"
                     /locus_tag="ACHKCL_002488"
     CDS             complement(18873..19451)
                     /gene="flhC"
                     /locus_tag="ACHKCL_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5763442.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19454..19804)
                     /gene="flhD"
                     /locus_tag="ACHKCL_002489"
     CDS             complement(19454..19804)
                     /gene="flhD"
                     /locus_tag="ACHKCL_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5763443.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4912 a         5583 c         4937 g         4924 t
ORIGIN      
        1 ccgctcaaca gatcgcggta atcgttcatt ttttatacta ttcagggata aaaaaaggcc
       61 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      121 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      181 ccgtctacga cataacgcga atactcgtcc gcgtgtgcat ctgatgacat ggacaatcct
      241 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      301 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc ggccataatg
      361 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
      421 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
      481 tttagagggt tagcgcacat atcaaaggcg cgatgaaaag tcactgccag cggaccggca
      541 gcagccatta ttttttccat tcgtgacata tcgacattac cgtcaacatc gagcacgccc
      601 gtcacgagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
      661 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
      721 atcgtcaccc gctggcgcac ggatttcagt acacccaacg acggcgttaa gcccccctct
      781 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagcgcgcat
      841 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgccgactg
      901 ctcgctggca acaatctctt caatggacca cggatgaaat ttaatggtcg ttttgccatc
      961 ggtgaccgcc agcgtcggat tcggtaatcg ttcatgctca ccttttggcg aactggtttt
     1021 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1081 cagcgtttct ggatcacccg gcaaaacgat ttcaatatgt tcccaacctt cgtgcgggta
     1141 acgtttttcc ctaggccacg gtagctccac aatagaaaac tgccagtgtg caacctgtac
     1201 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt ctgacaaaag
     1261 ctcgccacac tgttcaaacc cgcgacgcca gcgctcagca gtggcgtttt gatggcaacg
     1321 caaagaaatg tgatcggcag tcagcggagt gatattcaac cccagacggc gggaaagttc
     1381 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1441 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1501 cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1561 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     1621 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     1681 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     1741 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     1801 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     1861 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     1921 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     1981 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2041 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
     2101 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2161 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2221 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2281 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2341 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
     2401 atcgtctcaa cgtgacgctg acccatgatg acgtgatggg cgaaagcctc tacaacccaa
     2461 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2521 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     2581 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     2641 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     2701 acctgatgca ggcatgggct atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     2761 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     2821 cgggtggtac agtgaaactg gccgatctgc tggatgaagc cctggaacgt gcacgccgtc
     2881 tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg gctaacgcgg
     2941 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3001 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgcat
     3061 acacgcgtgt attgtccgtg ttccgtaaag cagaaattga cgaagagcaa ctggctgcag
     3121 ctccggttat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3181 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
     3241 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3301 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
     3361 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
     3421 gtgaagcctg atcaggttta gccgatcagg cttttttatt accatctaaa tgtattctga
     3481 aaataggcat gccattgttt tctcgctgtt gagtaagagg ccagaagcgt aatatccagc
     3541 tccagggaaa cggtaacggt tcaatttaag gaataccgca gtgtttaaat ttcttgtatt
     3601 aacattaggc attatctctt gccaggttta cgcagaagat acgcttatag taaatgatca
     3661 tgacatttca gccatcaaag attgttggca aaaaaattca ggtgatgata ctgacattaa
     3721 cgtgatcaaa tcatgtctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     3781 ttatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacctga
     3841 tactgaacaa cttaacttgc ttaaaaaatc acaacgagcc tggctggatt ttagggacaa
     3901 agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtaacc cttattctga
     3961 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4021 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatattt
     4081 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
     4141 ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc agcgataatt
     4201 cacaatcact tcattacgtt gtactttcag cgacggaatt aaccgcccac cgccaggcac
     4261 ttcccagata aagcgcaacg gttctgctgc cgctataccg gaaaaggcca cggtggttcc
     4321 gctctgccca tctaattcga cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc
     4381 aggtgtcggg ccgataagct gataccgcca cgccaccagc gtcatcaatc ctgaagcagg
     4441 ttgtcgcgta gaaagaggtg cagacgacat cgactcaccg cgatgattta gcgtaattcc
     4501 gacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa
     4561 tattgctaat aaggttctca tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg
     4621 ttatcagaca gttccagatt cgacagcacc actaactgcg gcaagctgcg gcgcagaaag
     4681 cgagacaata atggtcgcag cgcgtggttc accaataaaa ctggcggcgc acccaacatc
     4741 tcctgacggg atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc
     4801 agtccgcccc cgccctgcaa cgcctgtagc aacaaacgtt ccagcggtgt atcgaggcca
     4861 ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg tcccaacgcc
     4921 acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc
     4981 gtttcgagaa tagtgcgcat atcgcgaatc ggtacttttt catcgaggag attttgcagc
     5041 actttatgca gtgtggtgag cgtgacgacg ccagggacga gatcttccgt cagctttggc
     5101 atctcctggg cgacgcgatc cagcagctgt tgcgcctcct gacgaccaaa cagctctgcg
     5161 gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc ctcaaccact
     5221 gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat agcattcagg
     5281 ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt tccagggtta
     5341 atagccagcc agcgccccgg ataagcatca ccactgccta tctccacgcc tttcatcaaa
     5401 atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga
     5461 aatcccatct cctgggcaaa tttcttgcgg atactgcgta tacggcccaa caactcacca
     5521 tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc cagagaatct
     5581 tccagttgta catcgttcca cgtcgcttcg acaacggcat tattctctgc catttttacc
     5641 ggttttggtt cggcaggcgc tttttgttcg cgtccacgga tccaccaggc cagccccagc
     5701 aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag gccgagtaaa
     5761 ccgagcacgg cagcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc
     5821 tgctcgccaa catcctgatc ggtgctgaca cgcgtaacaa tgacccccgc ggcggtagaa
     5881 atcaccagag ccgggatttg tgccaccaga ccgtcaccaa tggtcaagag cgtataactt
     5941 tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa cccgccgaca
     6001 acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc
     6061 ccgtccattg agccgtaaaa atcggcttcc tgagtgactt cggagcggcg ttttttcgcc
     6121 tcatcttcac caatcaatcc ggcgttaagg tcggcgtcaa tcgccatctg cttacccggc
     6181 ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc tttggtaatg
     6241 accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc gaaattgcca
     6301 ccaacgagga agtgaccgaa cgcttcaacc accttccctg ccgccgctgc gccggtatgc
     6361 ccttccatta aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa cagcgtggta
     6421 aacaacaaaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
     6481 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
     6541 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
     6601 tgccattgtg tcgatttcag gtttgtgggc aggcgcagca tcgcggccag attactcatg
     6661 ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac
     6721 agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg ccagcacttc
     6781 cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccacat gtcgatacag
     6841 cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg
     6901 aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt
     6961 ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga catccgcttt
     7021 cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc tgcatctgac ggatccgccc
     7081 tttaacatgg gggtcgcctt cgctttgttt gaactcatca cgaatatcct gccgcgacat
     7141 tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca ccattggaat
     7201 gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat tacccatggc
     7261 ggtaatcgga gactcggcca ttaagcgcat catatgcggc cagtgatgcc agagaaaaaa
     7321 ccccgtcacg ctgccaacca ggatggtttt caaaattgct ttaagcaact ccgcgccagt
     7381 ctgtgccgag aacatccgtt ttatgcccgg tagcgggttg agtttggaaa acttcggctg
     7441 caaggatttt ccgctaaata ccagcccccc cagcataacc ggagaaatga gcgccaccag
     7501 caccacgccg ctaatcagcg gtagcagcgc cagcatggct tctctgatca gcagaataat
     7561 ttgcccgagg atcagattcg ggtctttgat aatactgtga tcaaaatgca gcccagcgga
     7621 gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa cactaacgcc
     7681 caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc cctcttcccg
     7741 cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac
     7801 gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg
     7861 gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gctgccacac atcaagcata
     7921 gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac cgcctgatat gacgtggtca
     7981 cgccacatca ggcaatacaa atcaaaatcc aagactatcc aacaaatcgt ccacctgatc
     8041 ctgactggct accacaccgg ctttgctggt atcgacctga ggtccattaa gcaaactctg
     8101 gttttcacgt tttggacgcg actcctgttc cgggatgttt tccaacagca ccatcagcaa
     8161 ctggcgttcg atctcctgaa tgacatccat catccgctta atgacctgcc cggtgagatc
     8221 ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg ttagtaaagc tggtatgcgc
     8281 gggtacatct gccagaaatt tccgcgtatc tgttaccagt tcacgggcgt cggcaaggtc
     8341 aatcggatcg gcaaaccagt catcccaacg ttgggttaac gcttttgctg atttctccat
     8401 ttgatcctga tgcggttgtg acgcctcaac actgttcagc gcccgctccg cagcctgggc
     8461 ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg gggatggctt ccgccgcttc
     8521 ggcaatggcc tgatccagtc ccagttcccg caaactgtcg cgcagcatac gcgtcaggct
     8581 gccgatgcgc gcaatgatat cgccagctga atgctcgtca gcaggtttga ttgatggttg
     8641 catcatagtc gcatcctcac atgcccagtt tctcaaagat tttgttgagt ttttcctcca
     8701 gcgtcgcggc ggtaaatggc ttcaccacat agccactggc ccccgcttgc gccgcagcaa
     8761 tgatgttctc tttcttcgct tctgcagtca ccattaacac tggcaatgcc gacatcgcgc
     8821 catccgcacg aatcgttttc agcaattcca ggccatccat gttgggcatg ttccagtcgg
     8881 agataacaaa tccataaccg cctgcctgca acttattgag agcgtcgacg ccatcttccg
     8941 cttcctcaac attattgaat cccagctctt tcagcaggtt acgcactatg cgtcgcatgg
     9001 tggaaaagtc atccacaacc aaaaatttaa gttctttatc cgccatttca cactcctgat
     9061 ttaaatacgt atcgcctgtc cggcactaat ttttgccaac atttgctggc ttacctggtt
     9121 aagatcgacc acttcgcaga caccacccat attgatggcc tcgcgcggca tgccgaacac
     9181 cacgcaactt gcttcgtttt gcgcaagggt ccatgcccca gcctgacgca tcgccaacat
     9241 tcccgccgcg ccgtcgttgc ccataccggt caggatcacc ccaaccgcat tacgccccgc
     9301 ctgtttggcg acagaatgga acaacacatc taccgaaggc cgatgacggt taaccgccgg
     9361 gccatcgtga attttgattt ggtaatttgc gccactacgc gccagctcca tatgccgatc
     9421 gcccggcgca atataggcat gccccggcaa aacacgttcc ccgtcttcgg cttctttaac
     9481 cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat
     9541 atgctgggta attaacagtg ccgggctgga aagcggcaac ggttgcagta cgtgacgaat
     9601 tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg
     9661 ccctgccttc agcgttgtcg gtgccgacaa tggcttatgt gctgcaaggc tcgcctttgc
     9721 tgctgtacgc accttttcag caatcatttc gctgtacgcc agcattcctt cgcgaatacc
     9781 cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca gcgtgacttc
     9841 tgatcctttg ccggtcaggg aagaaaccat cacaacgggc attggacgca aacgcattaa
     9901 tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg tcagcacatc
     9961 gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat
    10021 ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc
    10081 atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc atacaccgtt
    10141 tgaccacgca gcgtgaagcg gcgctcgagg tggctaaagt tttcagagtg acccgcaaac
    10201 agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg ctgggtagtt
    10261 tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc cggcacggtg
    10321 tactgtttcg ccagtagatt cagcgaggca aaatcaacat agttcgccag ctcctgacgc
    10381 acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg cagttgctgc
    10441 ggcgtcaggt ttttcaactc ttcatggcga tagataccgc ttctggcttt ttccagcact
    10501 tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca
    10561 gccagcgtca tcgcaatgct gtacggctct tcgccggtcg aagccgccgc gctccatacg
    10621 cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg atgtgcctca
    10681 cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca ctcaccgctg
    10741 tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc
    10801 aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat ggtcagccag aacgatcccg
    10861 gctcgttgat agatcaattg acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
    10921 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
    10981 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
    11041 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
    11101 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggtgttctg ctgtgtcacc
    11161 tgatccatct ggctgacagc ctgcgcaacc tgctcaatcc cccgccgttg ttcttccgag
    11221 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
    11281 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
    11341 tcgatcagcc ctttgatctc ttttgccgcc tgggcgcttc ggctggcaag attgcgcact
    11401 tctcctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttctactgcc
    11461 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
    11521 ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt gagtcatggt actgacctga
    11581 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcaa gttccgacgc ctgtcgtgcg
    11641 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
    11701 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
    11761 gcgataccaa tgtgcatctc ctggcttccc ttacgcacat cacttaccgt cccacgcaaa
    11821 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
    11881 gcaatcggac gcgccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
    11941 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
    12001 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
    12061 ctggcggctt ccagcacatg gatgatctcc agctgccagg cctcaaagtt aacgtcaaac
    12121 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
    12181 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
    12241 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
    12301 gtggattgcg tcagactggc acgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
    12361 tagctaagcg ctgtcagagt gcctgctttg ttcagcgtgg tactggcctg taacattacc
    12421 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgacctg attcagtcgt
    12481 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
    12541 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
    12601 ccccatcagg cggcaatgac cgcgttagta aatactcgtc aaaatgtttc ccagtttgga
    12661 tcttgttcag caattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
    12721 ggtgtttgcg gtttattggt gagtgggctg gctgccagac gaaacgcgga aaccgcttgc
    12781 gttaaacgac tagcctgttc ttccagcgca gctgcagcgg cagctgattc ctgcaccagc
    12841 gatgcgttct gttgcgtgac gtgatccatt tccgaaaccg ccaatgcgac ttgatcaatg
    12901 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
    12961 acagcatcga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
    13021 tcaacgcgcg agacggagtc ttcaatgaga gctttgatct cttttgccgc ctgggcgcta
    13081 cgactggcaa gattacgcac ttcgcccgcc accacggcaa aaccacggcc ctgttcaccc
    13141 gctcgcgcgg cttcaacagc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
    13201 tcgattacgc tgataatgtc ggcgattttc ttcgaactat cggcgatctc atgcatcgtt
    13261 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
    13321 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
    13381 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt ccgtacggga ggaaagatcg
    13441 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
    13501 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc
    13561 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
    13621 atgtgagcaa taatttttgc cagcggtgta agcaacatac ggcgaatgcc ataccacgcc
    13681 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
    13741 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
    13801 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
    13861 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
    13921 tatttttcat caatattacg actggtagcg accatttcag gtaacggtgc catgcttttg
    13981 aattttttat aatgagtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
    14041 actttggcat tactttgttg attggaggaa tccatcatca ttcgcaccgc tgaacgactc
    14101 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttct
    14161 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaga
    14221 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
    14281 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
    14341 aggaaggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaacccgatg
    14401 cgcagatcat cgggttcatt tcaattgagg aaatcaggag aattacgcca cttctgacgc
    14461 cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat
    14521 caacatacgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc
    14581 aaactctggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc
    14641 aaccacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt
    14701 atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacgcc
    14761 gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc
    14821 gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt
    14881 aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc
    14941 ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg
    15001 tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg
    15061 gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt aactacaacc
    15121 tgatgttgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag
    15181 atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg cgacgttgaa cactttccac
    15241 agttcgacga tgggcagata ttcaccccgc acttccagca cccgctcgcc gccggccagt
    15301 ggatgcagat cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg
    15361 aatacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt
    15421 aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcaacatg accgcccatc
    15481 tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc
    15541 tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg
    15601 ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc
    15661 cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga
    15721 attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg
    15781 tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctc
    15841 ttgtcgagct cggtggaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc
    15901 gccagatcac gcaccagccg gggatagcga ctaaagacat attccatcgg catcatgcga
    15961 atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggta
    16021 atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg
    16081 gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg
    16141 atgctggtgg attcactgct acgcgtcgtt ttttcccgct ccacgcgacc ggttggcgct
    16201 tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac ttctactgtt
    16261 tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg
    16321 gcgatatcgc ccggtaatat tgccgagaga gaatccgccc ctttcaccac gtcagttaac
    16381 gttgtcagat gccccagctc ttcttccagc aggtcgactt cgctggcctt caggcgcgaa
    16441 aggataattc gtcgcggcaa ctgactgcga ctctgctcat cttgcggttc acttttggca
    16501 accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa tgccagttga
    16561 cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga ctgtttataa
    16621 gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg
    16681 ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt
    16741 tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga
    16801 aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc
    16861 tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata
    16921 tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg
    16981 tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg cgttttcatg
    17041 caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac ggttgacggc
    17101 atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacca cacgtaacac
    17161 tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc
    17221 agaaagctcc cagttgctgt aacctttctc accactggcg taggggaaat catcggtatg
    17281 acccgaaagg ctaatacggt tgggaatacc attcagaacg ggcgcgatgg cacgcagaat
    17341 gtcgcgcata tagggttcga cgtcggcact gccagttcta aacatcgggc gattctggct
    17401 atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat gtggacgtaa
    17461 cgcccgcagt ttcggatcgg actctatcaa ttggtcgaga tcgccacgca atttccgcaa
    17521 tcgactttgc tccatgcgtt ttttcaactc ttcgatgttc ggctgcttat tcacttcccc
    17581 ctggctttgg gtgtaatcat cgccaccgcc gggaattggg ctttcactat tagaaatgcg
    17641 atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa tctgaatcaa
    17701 ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca tcatcgcggt
    17761 cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt
    17821 gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc
    17881 tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt
    17941 tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc
    18001 agattagaaa gcagtgtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc
    18061 tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat gccgaggaaa
    18121 gtccccacca tcgcatgtgc gataagcgcc cccagctcgg cggcaggacg atcggctgaa
    18181 cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc
    18241 ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct
    18301 tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga
    18361 taatcgacga taaaatcaag catgacgcta tccgcgatga tgcgtgggta gctggcgaag
    18421 atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc
    18481 gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat
    18541 ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc ctttaatcgc ttttccatta
    18601 ttgccgacaa taaacgaccc aatccctgca ccggcaataa tcaccagttc agcgggttga
    18661 tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca
    18721 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    18781 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    18841 cgctgctgga atgttgcgcc acaccgtatc agttaaacag cctgtactct ctgttcatcc
    18901 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    18961 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    19021 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    19081 accaatgtcc aggcacgggt taatgccagc agcggtcctt cttctgcttg cgggcactgt
    19141 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc
    19201 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaagtcata
    19261 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    19321 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    19381 gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt
    19441 ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat
    19501 tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg
    19561 aatcttgcgt caacagagta atcgtctggt ggctgtcaaa acggaagtga caaaccagct
    19621 gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaat gttgtcgcca
    19681 tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca atcagacgct
    19741 gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac tcggaggtat
    19801 gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg caccccgtga
    19861 tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga taagctgcaa
    19921 taagcagagc cacctttttg gtttaataag tccttacaaa tagaaggggg tctttacact
    19981 tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg
    20041 aggggggctg cgattttcaa taatgtgtga tgcagatcac acaaaacact caattactta
    20101 acataaatgt ataagtcata cttttgtttt gggtgtattt caatctgtta aaaagttttt
    20161 cgctacgcta gcaagcaaaa atgaaacagg aataatcgaa atgggatgtt gcgcacagtt
    20221 aaaataactc accgtaaata atcatctgct ataaataatc actttcatgc aataccagat
    20281 aagctatttt taaacagaca cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa
    20341 caatcaactt aatgtt
//
LOCUS       DAZFKY010000077        20327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFKY010000077 DAZFKY010000000
VERSION     DAZFKY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..646
                     /gene="ecpR"
                     /locus_tag="ACHKCL_002490"
                     /gene_synonym="matA"
     CDS             104..646
                     /gene="ecpR"
                     /locus_tag="ACHKCL_002490"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5763444.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            721..1308
                     /gene="ecpA"
                     /locus_tag="ACHKCL_002491"
                     /gene_synonym="matB"
     CDS             721..1308
                     /gene="ecpA"
                     /locus_tag="ACHKCL_002491"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5763445.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1366..2034
                     /gene="ecpB"
                     /locus_tag="ACHKCL_002492"
                     /gene_synonym="yagY"
     CDS             1366..2034
                     /gene="ecpB"
                     /locus_tag="ACHKCL_002492"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5763446.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2060..4585
                     /gene="ecpC"
                     /locus_tag="ACHKCL_002493"
                     /gene_synonym="yagX"
     CDS             2060..4585
                     /gene="ecpC"
                     /locus_tag="ACHKCL_002493"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5763447.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIATSGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4575..6218
                     /gene="ecpD"
                     /locus_tag="ACHKCL_002494"
     CDS             4575..6218
                     /gene="ecpD"
                     /locus_tag="ACHKCL_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5763448.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6187..6897
                     /gene="ecpE"
                     /locus_tag="ACHKCL_002495"
     CDS             6187..6897
                     /gene="ecpE"
                     /locus_tag="ACHKCL_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5763449.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7210..7539
                     /gene="ykgJ"
                     /locus_tag="ACHKCL_002496"
     CDS             7210..7539
                     /gene="ykgJ"
                     /locus_tag="ACHKCL_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5763450.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7787..8401)
                     /gene="yagU"
                     /locus_tag="ACHKCL_002497"
     CDS             complement(7787..8401)
                     /gene="yagU"
                     /locus_tag="ACHKCL_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5763451.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8819..9508
                     /gene="paoA"
                     /locus_tag="ACHKCL_002498"
     CDS             8819..9508
                     /gene="paoA"
                     /locus_tag="ACHKCL_002498"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO5763452.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9505..10461
                     /gene="paoB"
                     /locus_tag="ACHKCL_002499"
     CDS             9505..10461
                     /gene="paoB"
                     /locus_tag="ACHKCL_002499"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO5763453.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYTFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10458..12656
                     /gene="paoC"
                     /locus_tag="ACHKCL_002500"
     CDS             10458..12656
                     /gene="paoC"
                     /locus_tag="ACHKCL_002500"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5763454.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12666..13622
                     /gene="paoD"
                     /locus_tag="ACHKCL_002501"
     CDS             12666..13622
                     /gene="paoD"
                     /locus_tag="ACHKCL_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO5763455.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAGLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13801..14928)
                     /locus_tag="ACHKCL_002502"
     CDS             complement(13801..14928)
                     /locus_tag="ACHKCL_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763456.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKCYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15070..16128)
                     /locus_tag="ACHKCL_002503"
     CDS             complement(15070..16128)
                     /locus_tag="ACHKCL_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5763457.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16374..17276
                     /locus_tag="ACHKCL_002504"
     CDS             16374..17276
                     /locus_tag="ACHKCL_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5763458.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            17513..17575
                     /locus_tag="ACHKCL_002505"
     CDS             17513..17575
                     /locus_tag="ACHKCL_002505"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763459.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(17979..18257)
                     /locus_tag="ACHKCL_002506"
     CDS             complement(17979..18257)
                     /locus_tag="ACHKCL_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763460.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(18424..19146)
                     /locus_tag="ACHKCL_002507"
     CDS             complement(18424..19146)
                     /locus_tag="ACHKCL_002507"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5763461.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            19245..20144
                     /locus_tag="ACHKCL_002508"
     CDS             19245..20144
                     /locus_tag="ACHKCL_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5763462.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
BASE COUNT         5133 a         5360 c         5239 g         4595 t
ORIGIN      
        1 tccgatttac taaagaaact gaatgtacct gtaaaaatta caggtttgga aagtagtgac
       61 atggcaaaat gattacagca gggactatga ggttgaaaac catatggaat gtcaaaaccg
      121 ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact
      181 gattgtggat atcgacagat taatttatct atcgctggag aaaatcagaa aagatttcgt
      241 gtttatcaat ctcaatacgg attctttaac tgaatttata aaccgtgata atgagtggtt
      301 atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc
      361 aaattattgg tattacaaca gcaatattag gggcgtggta tacgctggac tgagtcgtga
      421 tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa
      481 agataaaatc actgaccggg aaatggaaat tatccgcatg acggctcagg gaatgctgcc
      541 taaatcgatt gccagaattg aaaattgtag tgtaaagaca gtgtataccc atcggcgtaa
      601 tgcagaggcc aagctgtact caaaattata taagttggtt cagtaaactc caggcaagtt
      661 agttttaaaa aatgactcac tgggacatca cgtcctcaat tcaactcggg aagaaataca
      721 atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggcat gggtgtggcg
      781 caggctgctg acgtaacagc tcaggctgta gcgacctggt cagcaacagc caaaaaagac
      841 accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta tgccgaaggc
      901 attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg tgactcaacg
      961 gctaccgcct ttaaactgac ctcacgtctt atcaccaaca cattaaccca gttggatacc
     1021 tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga aaaaactggc
     1081 gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag cccgctggca
     1141 aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacttt ctccatcatc
     1201 agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc ggaaggcatc
     1261 tggagcggcg acgttagcgt acagttcgac gcgacctgga ccagttaatc tctctgatgt
     1321 accagcaggg gtagcccccc agctttattc ctggacggaa ttgttatgaa aaagcacctt
     1381 ctgcttctcg ctctgctgtt atccggaata tctccggccc aggcgctgga tgtcggcgat
     1441 atatcatcgt ttatgaacag tgacagcagc acgctgagca aaacgatcaa aaacagtacc
     1501 gacagtggtc gccttatcaa tatccgtctc gaacggctct cttcaccgct tgacgacggg
     1561 caggttatct caatggacaa gccggacgag ttgctactca ctcccgccag cttgctgcta
     1621 cccgcccaag ccagcgaagt gatccgcttc ttctataagg gaccagcaga tgaaaaagag
     1681 cgctactacc gcattgtctg gtttgatcag gccctcagtg atgcgcagcg cgataatgcc
     1741 aaccgcagcg ctgtggccac tgcttccgcc cgcatcggca ccattctggt cgtcgccccc
     1801 cgccaggcaa actaccactt tcagtacgcc aacggcaccc tgacaaatac aggaaatgcg
     1861 acgctgcgga tcctcgccta cggcccctgc ctgaaagccg ccaatggtaa agagtgtaaa
     1921 gagaattact acctgatgcc gggcaagtcg cgtcgtttta cccgcgtgga cacggcggat
     1981 aacaaaggac gggttgcact ttggcagggt gataagttca ttcccgtgaa atagatagct
     2041 gtgcagatgg ataacgacaa tgcctttacg acggttctcc ccaggactga aagcccagtt
     2101 tgccttcggc atggtctttt tgttcgttca gcccgatgcc agcgctgctg acataagtgc
     2161 gcagcaaata ggtggggtga ttattccgca ggccttcagt caggcgcttc aggacggcat
     2221 gagcgtcccg ctctatattc atctcgccgg tagccagggt cgccaggacg atcagcgaat
     2281 cggcagcgct tttatctggt tggatgatgg acagctacgc atccggaaaa tacagctgga
     2341 agagagtgaa gataacgcca gtgtcagcga acaaactcga cagcagctga tggctctggc
     2401 gaacgccccg ttcaatgagg cccttaccat ccccctgact gacaacgcgc agctggatct
     2461 cagcttgcgc caactgctgc tgcagctggt ggtcaagcgc gaagcgctgg gcactgtact
     2521 acgctcacgt agcgaagaca tcgggcagtc cagtgttaac accctcagca gtaatctgag
     2581 ctataacttc ggcatctata acaaccagtt gcgtaacggc gggagcaaca catccagcta
     2641 tctgtcgctg aataacgtta ctgcactgcg cgaacatcat gtggtgctcg acggctcgct
     2701 gtacgggatc ggtagcggtc aacaggacag tgaattatat aaagcgatgt atgaacgcga
     2761 ttttgccggt caccgatttg ccggtggaac gctcgacacc tggaacttgc agtccttagg
     2821 gccgatgacc gccatttcag cagggaagat ttacggcctt tcctggggaa accaggccag
     2881 ctccaccatc ttcgacagca gccagtcagc cacgccagtg atcgcctttt taccggcggc
     2941 gggtgaagta catctcaccc gtgatgggcg gttactaagc gttcagaact tcaccatggg
     3001 caatcatgaa gtggataccc ggggtctacc atacggtatt tacgatgtgg aagttgaggt
     3061 gatcgttaac ggtcgcgtga tcagcaaacg cacccagcgg gtcaataagc tgtttagccg
     3121 ggggcgcggc gtcggtgcac cactggcgtg gcaggtatgg ggcggtagct ttcatatgga
     3181 tcgctggtcg gaaaacggga aaaagacgcg accagctaaa gagagttggc tggcaggtgc
     3241 ctcgacctcc ggctcactga gtacgcttag ctgggcggca acgggatatg gatacgataa
     3301 tcaggcggtg ggtgaaaccc gtctgacgct gccgcttggg ggagcgatca acgttaacct
     3361 gcaaaatatg ctggccagtg acagctcatg gagcagcatc ggcagcatca gcgccactct
     3421 accgggaggc tttagttcgc tgtgggttaa tcaggaaaaa acccgcattg gcaatcaatt
     3481 gcgacgtagc gatgccgaca accgtgctat cggcggcaca ctcaacctga actcactgtg
     3541 gtcgaagctg ggcacattca gcatcagcta caatgatgac cgccgttaca acagccatta
     3601 ttacacggca gattactatc aaaatgtcta cagcggtacc tttggttcgc ttggcctgcg
     3661 ggccggtatt cagcgctata acaacggcga cagcaacgcc aatacaggga aatatatcgc
     3721 tctcgatctc tcgctaccac tgggcaactg gtttagcgca gggatgactc atcaaaacgg
     3781 ctacaccatg gcaaacctgt cagcacgcaa gcagtttgat gaagggacca ttcgcactgt
     3841 tggtgccaat ctgtcacgag ccatctccgg cgataccggt gatgacaaaa ctctcagtgg
     3901 tggggcgtat gcacagttcg acgctcgcta cgccagcgga acgctgaacg tcaatagcgc
     3961 ggcggacggc tacgtcaata ccaatttaac cgccaatggc agcgtcggct ggcagggtaa
     4021 aaacattgct accagcgggc ggactgatgg caacgctggg gtgatattca acaccgggct
     4081 ggaggacgac ggtcagatca gcgccaaaat caacgggcgg attttcccgc ttaacggcaa
     4141 gcgtaactat ctcccgctct ctccctatgg aagatatgag gtggagttac agaacagcaa
     4201 aaactcactc gacagttacg atatcgtcag cggtcgcaaa agtcatctga ctctctatcc
     4261 aggcaatgtc gctgtcattg agccagaggt gaagcagatg gttaccgtct ccggtcgtat
     4321 ccgtgcggaa gacggcacac tgctggctaa cgcacggatt aacaaccata tcggccgaac
     4381 ccgaaccgat gaaaacggcg agtttgtcat ggacgtggat aagaaatacc ccactatcga
     4441 ttttcgctac agtggcaata aaacctgcga agtggcactg gaactcaacc aggcgcgcgg
     4501 tgccgtctgg gtcggtgatg tggtctgcag cggcctctca tcgtgggcgg cggtgacgca
     4561 gacaggagaa gagaatgaga gttaacctac tgatagcgat gataatcttt gcgctaatct
     4621 ggccagtaac tgcgctcaga gcggcagtga gcaaaacaac ctgggcggat gcaccggcac
     4681 gcgagtttgt gtttgtcgaa aacaactcag acgacaactt tttcgtcact cctggcgggg
     4741 cgctggatcc gcgcctgacc ggtgccaacc gctggaccgg tttaaaatac aatggttcag
     4801 gaaccatcta tcagcaaagc ctcggctaca ttgataacgg ttacaacacc ggcctttata
     4861 ccaactggaa gtttgatatg tggctggaaa attcaccagt ttcatctcct ttaactggct
     4921 tgcgctgcat caactggtac gctgggtgta atatgaccac cagtcttatc ctgccgcaaa
     4981 ccaccgacac cagtggattt tatggcgcga cggtgaccag cggcggcgcg aagtggatgc
     5041 acggcatgtt gtcagacgcg ttttaccagt atctgcaaca aatgcccgtc ggcagcagct
     5101 ttacaatgac catcaatgcc tgccagacct ctgtgaacta tgacgccagc agcggcgcac
     5161 gctgtaagga tcaggcctcc ggcaactggt atgttcgcaa cgtcacccat acgaaagcag
     5221 caaatctacg gttgataaat acccactcgc tggcggaagt atttatcaac agcgacggag
     5281 taccgactct gggcgaaggg aacgccgact gccggacgca aaccatcggc agccgttcag
     5341 gattaagttg taagatggtt aactataccc tgcaaacaaa cggactcagc aacacctcaa
     5401 tccatatatt cccggcgatc gccaactcgt cgttagcctc ggccgtcggg gcgtacgata
     5461 tgcagttcag tctgaatggc agttcatgga aaccggtgag caatactgcc tattactaca
     5521 ccttcaacga gatgaagagc gcagactcga tctatgtttt cttctcgagc aacttcttta
     5581 agcagatggt gaaccaggga atcagcgata tcaacaccaa agatctattc aactttcgct
     5641 ttcagaacac cacatcaccg gagtctggct ggtatgaatt ttctacctcc aacacgctga
     5701 ttatcaaacc ccgtgatttc agcatcagta ttatctccga tgaatatact cagacaccgt
     5761 cgcgggaggg atatgtcggc agcggcgagt cggcactcga tttcggctat atcgtaacca
     5821 ccagcggtaa aacagctgcc gacgaagtgc tgatcaaggt gaccggaccc gcgcaggtga
     5881 ttggcgggcg ctcctattgt gtcttcagct ccgatgacgg taaggcgaaa gtaccgttcc
     5941 cggcgacgct ttcctttatt acccgcaacg gagctacaaa aacctacgat gccgggtgcg
     6001 atgatagctg gcgggatatg accgatgcgc tgtggttgac cacaccgtgg actgatatct
     6061 ctggcgaagt ggggcagatg gataagacca cagtcaaatt ttcgattcca atggataacg
     6121 ccatttctct gcgtacggta gatgataacg gctggtttgg cgaagtcagc gcttcaggag
     6181 aaattcatgt tcaggcgacg tggcgtaaca ttaactaagg ccctgctgac agcggtctgt
     6241 atgctggcgg cacctttgac acaggcgatt tcggtcggca atctgacttt ttcactgccg
     6301 tccgagactg actttgtcag caaacgtgta gtgaataaca acaaaagcgc gcggatatac
     6361 cgtattgcca tcagtgctat cgatagcccg ggcagcagtg aattacgcac ccgaccggtg
     6421 gatggtgaac tgcttttcgc cccccgccag ctggcgttgc aggctggtga gagcgagtat
     6481 tttaaatttt actatcatgg gccacaggat aaccgcgagc gctactaccg ggtctcattt
     6541 cgcgaggtcc ccactcgtaa cctgacaaaa cgcagcccta ccggcggtga ggttagcacg
     6601 gagccggtgg tggtgatgga taccattctg gtagtacgac cacgtcaggt tcagtttaaa
     6661 tggtccttcg atcaggtgac cggaacggta agtaacaccg gcaacacgtg gtttaagcta
     6721 ctgattaaac caggatgtga ttcgaccgaa gaggaaggcg atgcctggta tctacgtccg
     6781 gaagacgtgg ttcatcagcc tgagttacgt cagccgggga atcactatct ggtctataac
     6841 gacaaattca ttaagattag cgattcctgt ccggctaagc ccccttcggc ggactgatac
     6901 ttatccatcg gtcgagggaa gaattccacc tcagagactc aaaaatcgtt taaatgatgt
     6961 ggaagcgatc gtcaatatca cctgtaacga taggctttct catatgcaca ccacagtgtt
     7021 caggagacac ggaaaggtac caggcggcct gctaacgagt ggtttcttca ctaattagga
     7081 cgtaaaatcg gtaacggctg gaaatcattc aatactcaca ctatcgaaag tacaccagcc
     7141 aaccgcgata cgtccctgca tacgacgtac cgcagttctc tttacgattt atagctgtac
     7201 tggtgaatta tgagcaatct gaatccatgc atgacgtgtg gtgcctgttg tgcatttttc
     7261 cgcgtctctt tctactgggc cgaagccgac gatgctggcg gcactattcc cgccaggctc
     7321 actgaacaaa tatccccttt tcaccgatgc atgagcggaa ctaatcagaa aaacccccga
     7381 tgtattgccc ttgcaggaac cccgggcaaa aatgcctgct gcacgatata taaaaatcga
     7441 tcgtccacat gcagagaatt cgccatgtct ggtgagaacg gagtcgtcaa tgaggcttgc
     7501 aatcgtgcaa gggctaaata cgggctgaca ccgttataaa tatacaataa ttatttgcac
     7561 tgccccgcca gctgtaatag cggggcttcg tttttcaggg gtaacaaacc ccgatacttc
     7621 ttctatttgc cagcaacaat gcctctcttc tgtagcgttc ttcggatcta catcatcctg
     7681 aagtagcgcg atatactcac tgactatctg aatcagaata taaaaagcaa tgtttttaac
     7741 ctataaaaat ggcgctgtat ttgcgccatt tttatcattc aatgcattat ctgtttgagc
     7801 ctaaagggat ctcagggtct ggctcatgag taattctgtt tcgtaaatct ctgcgaataa
     7861 tttcaatgga ccagaaccag actaaatgtc caaaaatttc agaaacattc tcataccacg
     7921 ggagatcaaa cagaggtggt gtcagtccca tgagagggaa tgaaatcata tgaacaaaaa
     7981 gttgggctaa agcacctgcc agtaagccct gccagagttt aatttttgga aatacttctg
     8041 cgaccacaca ataaccgaca gcaaacacta tcgaaaagat aatgtgcgta acaccaaccc
     8101 agttaaagac atgcccggca aaggtataaa cagccgcatt gggatctgtc agccccagcc
     8161 agtctcgaag aaaaatatac ggaggattga gaaaattacg cgagcaatca atttgcccag
     8221 cagccctgat taatgattcc gggccacacg ctgcattaaa catatccacc gggctacgtg
     8281 gcggcaatgg aacttcagcc ccccacttca caaatgcgga aacaacgcca gcaatcagcc
     8341 caatgaatgc agcaagacca taacgtctgc ggttcggtgg agtttgttca aatatattca
     8401 tatctaccct gcttgtacca ttatgttata cacctcttta ggagtattca taaaacaagg
     8461 taaatgtaaa gaactgtatt gttttgtata acaagatggt tgtctaatca ccaaagaata
     8521 taagcaccat catttctccc tatttttata ttagaagtga catagatttg cagggtgatg
     8581 cagagctgac aatcacaggt ttccttatcg gtttttgcat cgtacaacta aagcaataaa
     8641 ccagctccca tcatcatcaa catcccggcg aacatgaatt tattagccgg gctcatagct
     8701 gcattcgcgt tttaagaata atcctcctgc tgtcgccgac tatgcttaac gtttaaaaaa
     8761 gcatcagcac tctcgcaacg cactcttatt ttccccttta gaataccgga ggcctggtat
     8821 gagcaaccaa ggcgaatacc ccgaagataa tcgggttggg aagcatgagc cgcacgattt
     8881 aagtttgacc cgtcgcgatc tgattaaagt gagcgccgca acggcggcgg ccgccgtggt
     8941 ttatcctcat tctacgcttg cggcaagcgt tccggcagct acacccgcgc ctgagataat
     9001 gcccctgaca cttaaggtga acggcaaaac cgagcagctt gaggtggata cccgaaccac
     9061 gctactggac gctttgcgtg aaaatctgca tttgatcggt accaagaaag gttgcgatca
     9121 cggacagtgc ggagcctgta ccgtgctggt caatggtcgc aggcttaatg cctgcctgac
     9181 gcttgcagtc atgcatcagg gggccgagat caccaccatt gaaggcctgg gttcaccaga
     9241 taatcttcac cccatgcagg cggcctttat caagcatgat ggcttccagt gcggctactg
     9301 cacctccggg caaatttgct catcagtagc ggtgctaaaa gagattcagg acggcattcc
     9361 cagtcacgtc acggtcgatt tggtttcccc tccagaaaga actgccgatg agatccgtga
     9421 acgtatgagc ggcaacatct gtcgctgtgg tgcatacgct aacatccttg ccgccattga
     9481 agacgctgcg ggggagataa aatcatgaag gcgtttacct atgaacgagt gaacaccccc
     9541 gccgaggcgg cacttagcgc tcagcgcgta cccggcgcaa aatttatcgc gggcgggacc
     9601 aatctgctgg acctgatgaa gctggaaatt gaaacgccca cccaccttat cgatgtgaac
     9661 gggctcgggc tcgataagat cgaagtgacc gacgcgggtg ggctgcgcat cggcgcactg
     9721 gtacggaaca ccgacctggt ggctcacgag cgcgtgcgtc gtgattacgc ggtactctcc
     9781 cgcgccctgc tcgctggcgc gtctggtcag ttacgcaatc aggcaaccac agcaggtaat
     9841 ctgctccagc gcacgcgctg cccctatttt tacgacacca atcagccctg caataagcgc
     9901 ctgcccggga gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc ggtggtaggc
     9961 gtaagcgaag cctgcattgc cacccatccg agcgatatgg cggtcgcaat gcggttgctg
    10021 gatgcggtgg tggaaaccat cacgccggag ggaaagactc gcagtatcac actggctgat
    10081 ttttatcacc caccggggaa aacgccgcac attgaaaccg ccctgcttcc cggtgagctt
    10141 atcgttgcgg tgacgttacc tccgccgctc ggcggaaaac atatctaccg taaggtgcgc
    10201 gatcgcgcct cctacacctt tgccctggta tcggtcgcgg cgattattca gcctgacggc
    10261 agcgggcgcg tcgcgctggg cggagtagca cataagccct ggcgcattga ggctgcggat
    10321 gctcagctat cccagggggc gcaggccgta tatgacgcgc tgttcgccag cgcccatccc
    10381 accgctgaaa acacctttaa actcctgttg gcgaagcgaa cgcttgcctc cgtactggct
    10441 gaagcgaggg cacaagcatg aaatttgata aacccgcagg ggaaaacccg atcgatcagc
    10501 tgaaggttgt cggtcgtccc catgaccgca tcgacggacc gctgaaaact accggcacgg
    10561 cacgctacgc ctacgaatgg catgaagaat cccccaacgc cgcctatggc tatatcgtcg
    10621 gttccgccat tgccaaagga cgcctcaccg cccttgatac ggacgccgcg caaaaagcgc
    10681 cgggcgtact gcctgtcatt accgccagta acgccggggc actcagtaaa ggcgacaaaa
    10741 acaccgccag gctgttaggc ggccccacta ttgagcacta tcatcaggcc attgctctgg
    10801 tagtggccga gaccttcgaa caggcgcgag cggcggcctc gctggtgcag gcgcactatc
    10861 gccgtaataa aggagcttac tccctggcgg acgaaaaaca ggccgtcagt cagccgccgg
    10921 aagacacgcc cgacaagaac gtcggtgact ttgacggggc tttctcctcc gctgcggtga
    10981 agattgatgc tacctacacg accccggacc agagccatat ggcgatggag ccgcatgcct
    11041 cgatggccgt ctgggatgga aataagctta ctctctggac ctcaaatcag atgattgact
    11101 ggtgccgcac cgatctggca aaaacgctga aagtgcccgt ggagaatgtg cgtattatct
    11161 ccccgtatat cggcggaggg tttggcggca agctgttcct gagaagcgat gcgctgctgg
    11221 cggccctcgc cgcccgagcg gtgaaacgtc cggttaaagt gatgctcccc cgcccctcta
    11281 ttcccaataa caccacgcac cgccccgcca cccttcagca cttgcgtatc ggtgccgacc
    11341 agagcgggaa aatcaccgct atctcacatg aaagctggtc tggaaacctg cccggcggca
    11401 cgccggaaac ggcggtacag caaagcgaat tactctacgc cggggcaaac cgtcataccg
    11461 gcctgcggct cgccacgctt gatttgccgg aagggaacgc catgcgtgcg cccggcgaag
    11521 cccccggtct gatggcgctc gaaatcgcga tcgacgaact ggcggaaaaa gcgggcatcg
    11581 atcccgtcga gtttcgcatt ctgaatgaca ctcagattga ccccgccgac ccgacgcgcc
    11641 gcttctctcg ccgtcagctt atcgagtgct tgcgcaccgg agcggataaa tttggttgga
    11701 agcagcgcaa cgccacaccc ggacaggtgc gcgacgggga gtggctagtc ggccacggcg
    11761 tcgcggcggg ctttcgcaat aatctgctgg aaaaatcggg ggctcgggtt cacctcgaac
    11821 caaacggcac cgttaccgtg gaaacggaca tgaccgacat tggcaccggc agctacacca
    11881 ttctggccca gacggcagcg gaaatgcttg gcgtaccgct ggagcaggtt gcggttcacc
    11941 tcggcgattc cagtttcccg gtttctgcgg gttctggtgg acaatggggc gcgaatacct
    12001 ccacctccgg cgtttacgcc gcctgtgtga agcttcgcga aatgattgcc tcggcagtcg
    12061 ggtttgatcc tgagcagtcg cagtttgccg acggcaagat taccaacggt acccgaagcg
    12121 ccatactaca tgaggccacc gcaggcggca gactgacagc ggaagagagc attgaattcg
    12181 gaacactgag caaggagtac cagcagtcga cctttgccgg gcattttgtg gaggtcggcg
    12241 tgcatagcgc gacgggagaa gttcgggtcc ggcgtatgct cgctgtgtgt gctgcaggac
    12301 gcatcctgaa tccgaaaact gcacgcagcc aagtcattgg cgcaatgact atgggcatgg
    12361 gcgcggcact gatggaggag ctggcggtgg atgaccgttt gggctacttc gttaatcacg
    12421 atatggcggg gtatgaggtg ccggtccatg cggatatccc aaaacaggaa gtgattttcc
    12481 tggatgatac cgaccccata tcctccccga tgaaggccaa aggtgtcggt gagctgggcc
    12541 tgtgcggcgt gagcgcggct atcgccaacg cggtgtataa cgccaccggt attcgggtac
    12601 gcgattatcc catcactctg gataagctgc tcgataagct gccggatgtg gtttaaggag
    12661 gaacaatgtc atacccgctt tttgataaag acgaacactg gcataagcca gagcaggcgt
    12721 ttctcaccga tgaccaccgg accattctgc gcttcgccgt agaggcgcta atgtccggta
    12781 aaggagcggg gctggtgacg ctggtggaga tacgcggcgg cgcggcgcgc ccgctcgggg
    12841 cgcagatggt ggtgcgcgaa gatggtcgtt actgcggttt tgtctctggc ggctgcgtgg
    12901 aggccgctgc cgcttttgaa gcgctggaga tgatgggctc aggctgcgat cgcgaaattc
    12961 gctatggcga aggttcgccg tggtttgaca tcgttctgcc ctgcggcggt gggatcacgc
    13021 tgacgctcca taaactacgc tcggcacagc ctctgctcgc cgtgctgaac cgcctggaac
    13081 agagaaaacc ggcggggctg cgctacgatc cgcaagcaca atcgttggtg tgcctgccca
    13141 cgcaaacccg aacgggctgg aatctcaatg gctttgaggt ggggttcagg ccatgcgtca
    13201 ggctgatgat ttacggacgt tctcttgagg cgcaggcaac cgcgagtctt gcagcagcca
    13261 caggctatga cagccatatc ttcgatcttt ttccggcctc agccagcgct cagatcgata
    13321 ccgatacggc ggtcattttg ctgtgccatg atctcaaccg ggagctgcca gtgttgcagg
    13381 ccgcgcgaga agcaaaaccc ttttatctcg gcgcattggg cagctatcga acccacacgt
    13441 tacgtctgca aaagctccac gagctgggat ggtccaggga ggagacagcg caaatccggg
    13501 cacccgtcgg gatattcccc aaagcccgga atgcgcatac actggccctc tccgtgctgg
    13561 cagaagtcgc ctctgtacgt ctccatcagg aggaggattc atgcctgccc ccgtcgtcct
    13621 gatccttgcg gccgggcgtg gaaagcgctt tctcacctcc gggggaaata ccataaatta
    13681 agagaaaaat ttgcagtagc cgttttagaa aatcacctaa atcgtttaaa agagcgccct
    13741 acccatagtg gttgctcatc gccttgtgta actttttcag caacgcgcgc cctcgccgga
    13801 ctaaagcgca ccggccttct ggcgtgctgt aacaaaggtc agtacgcccg cgcaaaggag
    13861 cagcacacta ctcatggcaa acgtactctg ccagccaagg cggtcaaaca cgatgcctcc
    13921 tgccgttgat ccaagggcga ttgagagctg aatcaccgcg accatgagcc cgcctccggc
    13981 ttcggcgttg tcaggcaacg tacgcgcaat ccatgtccac catccggttg gcgcggcggt
    14041 ggccagcatg ccccagagtc ctaacagcag cgagaccatc cagacgtgat gcccggtaag
    14101 catcagaccg atggcaatga cggccatcag tccgggtatg gccatcagcg tggggtagca
    14161 ttttctgttg agaaatatcg aaacaaccag cgtgccaata aaaccggcca cgccgataat
    14221 cagcaaaatc aacgacaagc ctaaggaatt aacccgtgtc accgactcca gaaatgggcg
    14281 cacataggtg aatagcgcaa actgccccat gaaaaacagg ccacaagcta tcatgccgac
    14341 cacaaccatg cggcggctaa ataagcggaa taccgcgcca catgacgtgt ggcttttatt
    14401 cgcatccatg ctgggcagac taatgcactg ccagataaag gcgacaaccg ccatgggcac
    14461 caagcacaag aaagcgcctc gccatccgac cgtagccccc agatagcttc ccagcggcgc
    14521 ggccacgacc gtcgccagcg cattaccggc gttgaaaatc gccagcgcac gcgtgacctg
    14581 atgctgcggc accagacgaa tcgccgttgc cgctgacatc gaccagaatc cgccaatggc
    14641 cacaccaatc atcgcacgac cgaccatata catcagataa ctggtagcca gcgcaataat
    14701 aagccccgat acggccatca gaaccgtcat ccccagcagc aggaacttac gattcatctt
    14761 tccagctagc gttgaaagcg tcaggctggt cagaaccgcc agcgcaccgg agatagcaat
    14821 cccccgccct gccagcccct ccgtcacgcc caggtcacgg gcaatcggcg tgagcaggct
    14881 gacgggcata aattcagaag caatcagcac aaacacgcac agggtcatgg caaaaatacc
    14941 gccccagtat gcgtgctgat gatgaggtga tggtgtttgg cttagtgtcg acatagtgaa
    15001 atttcacaaa gatcaggata aaagccaccg gccatagacc ggtggctttg catcaattca
    15061 ggggaggttt tatttcagtt tggtctggaa aaattgttcg aacttcgcga aaggtatttt
    15121 tccggccacg ttatcgtaca ggtccacgtg atttgcccct ggtaccacca ccaactcttt
    15181 gtctttactg ccaaccgcct tgtaagcatc ttcagcaaaa tagcgtgaat gtgctttctc
    15241 accggtcaca atcagcgtcg gaatggtgat ttcactggcg tagctcaaca gcggcatatt
    15301 cataaatgac agcggcatcg ttgccgtcca cgccccggta gagttaacgg aacgttcgtg
    15361 gaagccgcgc ggcatgcgat agtagtcaaa gaactctttc agtaccggat gaggatttgc
    15421 cggcagcgtt tctggcagaa tgcgctcgcc cgcactgacc ttgccgttgc tatcgacatt
    15481 aatatcatga ccaccgtgag cgaacgttcc gctctgcgca tccttccagc gctgttcatt
    15541 caaatattgc agaacagcac gacggtcggc ggtagaataa cggtcttcgc catcccccac
    15601 accatggccc atcgcccggc tcatgtcata catcacgctg gtagcaaccg ctttaacgcg
    15661 ggtatccatc gccgcgtcgt tcagcgccat gccgccccag ccacaaatac cgagcagccc
    15721 gatacggttg cgatctacct ctttttgcag ccctaagaaa tccaccgccg cgctgaaatc
    15781 ttcagtgttg atatccggtg aggcgacatt tcgcggatag ccgccgcttt ccccggtgta
    15841 agaaggatcg aacgccaggg taacaaaccc tcgttccgcc agcgtctgcg catacaagcc
    15901 gctggattgc tctttcaccg cgccaaatgg cccactgacc gcaatcgccg ccagcttgcg
    15961 atcgccacgg tctttaggca ggtaaagatc gcccactaag gtgatgccat atcggttcgg
    16021 gaacgagact ttacggtgat cgactttctg gctctcggcg aacgttttat cccatttatc
    16081 ggtcatggac acaggggcat taggattggt tgaatcagca taactcattg tcgtgactcc
    16141 acttaatgat gcgaaaagca gcatgaccgg catcgcggtt tttagcgttc gggtgaaggc
    16201 tttcataaaa atccccataa aaaacaacag agagatacag gtttccgacg gacaacgctg
    16261 cggtgcttta ctttgatggg gtcattatag tgagcagaat tagtgctgat tagggggcga
    16321 ttaatgctaa gattaatacc atattgttat aaattaatgc tgagattgcc ggcatgaagg
    16381 ggaatgtttc aatggcaaaa agggagaact acaacgagct atacctgttt atgcaggtgg
    16441 tgcgggaagg cagctttacc gcagcggcta agcggctggg tcttgctcag tcaggagtca
    16501 gccgttctgt tcgcgagctt gaagaaaggc tgggcgtcca gctgctggtg cgcaccacgc
    16561 gtaaactgtc gctgacgcaa gcaggcgagc agctctacca gaagacggca tctggatttg
    16621 aaatgttaga tcttgggctt gccacgctgg cgcactatcg agagacgccc tccggcacgg
    16681 tgcgtatcaa tgccagccag cacgcgattg ataaatgcct gctgccaaag ctggcggtat
    16741 ttaaacagcg atatcctgat atccagcttg aactgataaa tgagagccgg ttcgtcgata
    16801 tcatcgagga aagattcgat gcaggtgtcc gcctggggcc tgaagtcagc cagggcatgg
    16861 tcgcggtacg catcacgccc gatatggaga tggcaattgt ggggactccg gaacattttc
    16921 gtcgctatgg ttttccgcaa accccggcgg atttaaaggc gcatccctgt atcgcctatc
    16981 agtttgccga cggcagcgta taccagtggg aactcaatca ggatgataaa aaaatcactc
    17041 atcaacccga aggacaatgg gccttttccg acagctatat ggaagcagaa gccgcccggc
    17101 tgggcctcgg gttggcctat gttcctgttg aactggtcgc ggatgattta gaacacggta
    17161 agcttatcag agtgttgcag cgttacagcc tgcgaatgga gggattgttt ctctattacc
    17221 ctcatcgcaa cgtctccccc gctctgcgga tggtcattga tacattgaaa atctgaacgt
    17281 taacaacgac cgactcttca ctgtgcaata ttgtgtcagg tagtcactct gcgttcaatt
    17341 ccaggggccg ctttgagcga gaaagagcgg acttatctgt acaaggggac tatccagcaa
    17401 tggagatcat ccaaaagaaa aaaccacctg gctcccctcg acaaccttaa tccgggcatt
    17461 tagcctcaat caacattttc atatctgtca ggttacttca ttatgaaaac cgatcaatac
    17521 cgttgttttt gttgcaacag tagaatacgc gtatttatcc ctccatttct attaataacg
    17581 gcaataaact gttcatttca ctgatattta aaatatgcat ccttttcaag ctgttcagcg
    17641 gcgtaagaaa acgtattaaa tatgaattcg acgatttaac catttctgtc tgattggtaa
    17701 tcatctgtga ttaatgctgg tctattgatt actccagcat taaccgctta gggatgtatt
    17761 cttcctgttg atatacagcc ctcgaaaaag agagaaaata aaactgtgtc caatattact
    17821 ctaccactcc agaccgaagc atcggtagaa ttagcagaac gactggtaca atagattggg
    17881 atagcgtcat ttgtcaggta ttccattgcc tgacaaatga caattttgag tcaatagacg
    17941 tgcttcttga tcatgtctta aaaattatac catgagcctt atgaagcatc atttttactg
    18001 actggtgtta actttgctat catataaact ctgttcttaa aaaacgcgcc cagaatttta
    18061 tatgatttcc cttgtttttc tgcctgtaaa gaaatttctt tttcagtgct gttaatggtt
    18121 gaaccgacgg cagtaatcgt tttggcgacg ctactgaatg ataataaaga cagtgcaacc
    18181 acaacaatta aagaacagat agtattcacg ctaatttccc tgacaattga gaggatgagt
    18241 tttatatata aagacattaa acatgagtca tgtttacttc ctgtgaaaaa taatctttct
    18301 gtaattaaac cttcatctcc ttcggggtga gcaatacata atgtatcttg ctatgaaaaa
    18361 cacagggata tataaatatt ccttctgacc tatttaataa aacgtgaatg taaaagttat
    18421 tcttcagttt gcagatgcag tgggtctgat ggtaatttcg gttgtatcaa cgctttcggg
    18481 agattcaata atatgcctta cagcacgggc aatatccgcg ggttgaagtg caacagaacg
    18541 gtaggcgtcc attgccttca tggtttccac atgggtaatg gttgaagcca gttcactttc
    18601 gacaacgcca ggatttacgc aggtcacacg gattttcgag ctttcctgcc gtaagccatc
    18661 agaaatagcc cgtactgcaa attttgatgc acaatagact gcgcctgtgg gcacaacaga
    18721 aagggcacca atagaaccaa gattgattat ctgaccggaa ccctgtgctt ccatcaccgg
    18781 aagtacagcg ccaattcccc acagtacacc tttgatattc acgtcaatag tgagcgccca
    18841 ttcatcttgt tttcctgctg caagcggtga aagcggcata acgcccgcat tattgataag
    18901 aacatcaact cgcccccagc tatccagcgc tgcttgcacg aaatcggcca tggactgtcg
    18961 gtctgtgaca tccaactccc gcgctttagc aattcctcct gcgcggcaga tttcggttgc
    19021 aatggcttcg atacgctcaa ctctgcgtgc tcccagtaaa acctttgcac ctgtcattcc
    19081 aagctctctg gcaatacctt ccccaatacc acttgatgcg ccggtaatta atatgacctt
    19141 atccattacg ttctccttca aatgtgttcg gagagagaat agaactttat gattggttcc
    19201 agtatcctgc tttcaatgta ctcagtggtt agcggcatta aacaatgaaa atcgatctta
    19261 atcttttacc catttttatc gcggtagcag aagagagtaa ttttagtaaa gccgccgcac
    19321 ggctgggcgt tacccgttca gcggtcagtc agggaataag acgccttgaa gatgcattcg
    19381 ggacaatgct tgtaatgcgg accacgcgct cagttaattt gacagaagcg ggagaacggc
    19441 tgcataaatc gctgtcatta cctttatccg gtattgaggc agcgttcgaa gagatggttt
    19501 ctgaccatgt gccacgcgga cttctcagaa tcgcggtgac gtcaatagct gaagaatttc
    19561 tttcaggtcc gctaatcgct tcgtttgcag cagccaatcc agccgttacg cttgatattg
    19621 ttgtcacgga tgaggaattc gatatcgtgg ccgcaggtta cgatgctggc gtaaggctcg
    19681 gagaggtcat tgaaaaagac atgattgctg tgccccttac cggcaggcag cgtgaaatgg
    19741 tcgttgcctc tccctcctat ctcgcagcca acagcacacc tgttcatccg cgtgaacttg
    19801 tgaatcataa gtgtatcggc tggcgtcagt ccccggaggt tgcgccttat cgctggccat
    19861 ttgaagaaaa cgggagagct ttcgaccttg cgattgaacc gcagattacg actaatgatc
    19921 tgcgtctgat gttgaggctg gctcttgccg gcggaggaat aacaattgcc actcaggaaa
    19981 ctttcaggcc gtatattgaa agcggtaagc ttgtatctct gcttgatgac tttcttccac
    20041 aatttccggg cttctatctg tatttcccac agcgtcgcaa tattgcacca aagctccgcg
    20101 ctctgattga ccacgttaag gaatggcggc agcaattggc ttaaatgtct gcacctgcat
    20161 tgcctgatgt cagaacagta ttttgattaa ttgccagggt tacaatggca caaatgcggc
    20221 acaggaggaa acgtggtgta tttaaatatg gggtaactac ttgatttaaa tggtgcgata
    20281 ataggaacca ataatcgcca ccaacacaat gattttaata cataaat
//
LOCUS       DAZFKY010000078        20307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFKY010000078 DAZFKY010000000
VERSION     DAZFKY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            318..1184
                     /gene="folD"
                     /locus_tag="ACHKCL_002509"
     CDS             318..1184
                     /gene="folD"
                     /locus_tag="ACHKCL_002509"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5763463.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1186..1398
                     /gene="ybcJ"
                     /locus_tag="ACHKCL_002510"
     CDS             1186..1398
                     /gene="ybcJ"
                     /locus_tag="ACHKCL_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5763464.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1506..2027
                     /locus_tag="ACHKCL_002511"
     CDS             1506..2027
                     /locus_tag="ACHKCL_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5763465.1"
                     /translation="MPTVITHAAVPLCIDLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2063..3448)
                     /gene="cysS"
                     /locus_tag="ACHKCL_002512"
     CDS             complement(2063..3448)
                     /gene="cysS"
                     /locus_tag="ACHKCL_002512"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5763466.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3622..4116
                     /gene="ppiB"
                     /locus_tag="ACHKCL_002513"
     CDS             3622..4116
                     /gene="ppiB"
                     /locus_tag="ACHKCL_002513"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5763467.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4119..4841
                     /gene="lpxH"
                     /locus_tag="ACHKCL_002514"
     CDS             4119..4841
                     /gene="lpxH"
                     /locus_tag="ACHKCL_002514"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5763468.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4959..5468
                     /gene="purE"
                     /locus_tag="ACHKCL_002515"
     CDS             4959..5468
                     /gene="purE"
                     /locus_tag="ACHKCL_002515"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5763469.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5465..6532
                     /gene="purK"
                     /locus_tag="ACHKCL_002516"
     CDS             5465..6532
                     /gene="purK"
                     /locus_tag="ACHKCL_002516"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5763470.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6671..7564)
                     /locus_tag="ACHKCL_002517"
     CDS             complement(6671..7564)
                     /locus_tag="ACHKCL_002517"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5763471.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7561..8376)
                     /locus_tag="ACHKCL_002518"
     CDS             complement(7561..8376)
                     /locus_tag="ACHKCL_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5763472.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8387..9646)
                     /locus_tag="ACHKCL_002519"
     CDS             complement(8387..9646)
                     /locus_tag="ACHKCL_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5763473.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9656..11323)
                     /locus_tag="ACHKCL_002520"
     CDS             complement(9656..11323)
                     /locus_tag="ACHKCL_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5763474.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11640..12689
                     /gene="allD"
                     /locus_tag="ACHKCL_002521"
     CDS             11640..12689
                     /gene="allD"
                     /locus_tag="ACHKCL_002521"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5763475.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12711..13946
                     /gene="allC"
                     /locus_tag="ACHKCL_002522"
     CDS             12711..13946
                     /gene="allC"
                     /locus_tag="ACHKCL_002522"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5763476.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13957..14742
                     /gene="allE"
                     /locus_tag="ACHKCL_002523"
     CDS             13957..14742
                     /gene="allE"
                     /locus_tag="ACHKCL_002523"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5763477.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14970..16115)
                     /gene="glxK"
                     /locus_tag="ACHKCL_002524"
     CDS             complement(14970..16115)
                     /gene="glxK"
                     /locus_tag="ACHKCL_002524"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5763478.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16137..17438)
                     /locus_tag="ACHKCL_002525"
     CDS             complement(16137..17438)
                     /locus_tag="ACHKCL_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5763479.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17495..18856)
                     /gene="allB"
                     /locus_tag="ACHKCL_002526"
                     /pseudo
     CDS             complement(17495..18856)
                     /gene="allB"
                     /locus_tag="ACHKCL_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18893..20284)
                     /locus_tag="ACHKCL_002527"
     CDS             complement(18893..20284)
                     /locus_tag="ACHKCL_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5763480.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
BASE COUNT         4941 a         5593 c         5142 g         4631 t
ORIGIN      
        1 atgaatactt ccgcgcaaaa tatattcaac acaaatatag acctgaagcg gtaaattacc
       61 aggctgaaaa ttctttttat attgtcaggt atttcttaaa ttatcttaat ccttaaacaa
      121 ggaaataaat cagttccaga tttacaacgc catcatggac gaaaaatgaa gctttcaatc
      181 tcagcgacag tgcgcctaac cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa
      241 acctgacagc gcgccccgct tctgacaaaa taggcgcatc cccttcgacc tacgtaacag
      301 atggaatcct ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg
      361 tgcgctctga agttgctcaa aaagttcagg cgcgtattgc agccggactg cgggcaccag
      421 gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac
      481 gtaaggcttg tgaagaagtc gggttcgtct cccgctctta tgacctcccg gaaaccacca
      541 gcgaagcgga gctgctggag cttatcgatg cgctgaatgc cgacaacacc atcgatggca
      601 ttctggttca actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtatac
      661 acccggacaa agacgtagac ggtttccatc cttataacgt cggtcgtctg tgccagcgcg
      721 cgccgcgtct gcgtccctgc accccgcgcg gtattgtcac gctgcttgag cgttacaaca
      781 ttgatacctt cggcctcaac gccgtggtga ttggcgcatc gaatatcgtt ggccgcccga
      841 tgagcatgga actgttgctc gcaggttgca ccaccaccgt gactcaccgt ttcactaaaa
      901 atctgcgtca ccacgtagaa aatgccgatc tgttgatcgt tgccgtcggc aagccgggct
      961 ttattcctgg tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc
     1021 tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat
     1081 acattacgcc tgttccgggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca
     1141 cgctacaggc gtgcgttgaa tatcatgatc cacagggtga gtaacatggc gacattttct
     1201 ttaggtaaac atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa
     1261 agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggcgcggtt
     1321 gaaacgcgca aacgctgcaa aatcgtcgcc gggcagacag tgagttttgc aggtcacagc
     1381 gtacaggttg ttgcctgatc cctttcccgg cctgtatcaa caggccggtt ctcttttcag
     1441 acttttgccc tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga
     1501 ccatcatgcc aaccgttatt acgcacgccg ccgttcccct atgcattgat ttagggttgg
     1561 gttcgaaagt catccctccg cgcctgttat ttgccggaat catcctggcg atgctgcccg
     1621 atgccgacgt cttgtcgttt aattttggcg ttgcttacgg caatgttttt ggtcatcgcg
     1681 ggtttaccca ttcgctggtg tttgcgtttg ttgtcccgct gttatgtgtg ctgattggac
     1741 gtcgatggtt cagggctggg ctgattcgct gctggctgtt tttaaccgtc tcgttgctat
     1801 cgcacagctt gctggattcg gtaaccactg gcggtaaagg ggttggctgg ctgtggccgt
     1861 ggtcagatga acgctttttc gctccctggc aggtgattaa agtcgcgccg tttgcattgt
     1921 ctcgttacac cacagcgtac gggcatcagg tgattatttc tgaattgatg tgggtgtggt
     1981 tgccggggat gctactgatg ggaatgttgt ggtggcgcag acgataaccg gatgcgaaaa
     2041 ctcgcatccg gcaatagcgc aattacttac gacgccaggt ggtcccttgc gggccatctt
     2101 ccagcacgat ccccatctca ttaagacggt cacgcgccgc atctgccgcc gcccagtctt
     2161 tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc aatctcagcc acttcgctgt
     2221 cgtctgcctg cgcgccgctt tgcaggaacg cttccggttc ttgctccagc aggcccaata
     2281 cggcggaaag tttacgcagg tgagatgcca ttgcattcgc cgctgccata tcttctactt
     2341 tcagacggtt tacttcacgc gccatatcaa acagcacgga ataggcttcc ggggtgttga
     2401 aatcgtcgtc catcgcttca ataaagcgcg cttcaaacgc ttcgccaccg gcaggcgcaa
     2461 cggttttatc tgtgccgcgc agcgcagtgt agagacgctc cagcgccgca cgcgcctgct
     2521 tcaggttctc ttcgctatag ttcagctggc tgcgatagtg gcccgacatc aggaagtaac
     2581 gcacggtttc cgcgtcgtag tatttcagca catcgcgcac ggtaaagaag ttacccagcg
     2641 atttggacat cttctcgcgg tcaaccatca ccatccccga gtgcatccag tagttcacat
     2701 actgaccatc atgggcacag gtggactgcg cgatttcgtt ttcgtggtgc gggaacatca
     2761 ggtctgaacc gccgccgtgg atatcaaagt ggttacccag ctgcttgcag ttcattgccg
     2821 aacattcaat gtgccagcca ggacgacccg cgccccacgg agacggccag ctcggttcgc
     2881 cctctttcga catcttccac agaacgaagt ccattgggtt gcgtttgtcg tcgaccacgt
     2941 caacgcgcgc gcctgcctgc agctggtcga gatcctgacg cgacagcacg ccataagttg
     3001 gatcggtcgg gacgtcgaac atcacgtcgc cgttgtccgc cacataagcg tgacctttgg
     3061 cgatcagttg ttcagtgagt tcaataattt ctgcgatatg gtgcgtcgcg cgcggctcca
     3121 tatccgggcg cagaatgttc aacgcatcaa aatctttgtg catttcggcg atcatgcgat
     3181 ccaccagcgc cacaaagctt tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga
     3241 tatcggtaat gttgcgcaca tacttcagct tatagccgag gaaacgcaga tagcgcgcaa
     3301 ccacgtcaaa agcaacaaag gtacgcccgt gaccgatatg acagagatcg taaacggtga
     3361 ttccacacac gtacatgccg acttccccgg cgtgaatagg cttaaattcc tctttttggc
     3421 gtgtcagagt attgaagatt tttagcatcg aagattccgt ttagacatgt gtgggtaatt
     3481 gagttgcgta taatacccat atttcccgcg cgaatcagca tacattgcgt gatgatccga
     3541 tcgtgcggtt atgctataac accaccctat atatgacccg aactgggttg aagcaccaat
     3601 caaacggaac aggatgcaaa aatggttact ttccacacca atcacggcga tattgtcatc
     3661 aaaacttttg acgataaagc acctgaaaca gttaaaaact tcctggacta ctgccgcgaa
     3721 ggtttttaca acaacaccat tttccaccgt gttatcaacg gctttatgat tcagggcggc
     3781 ggttttgaac cgggcatgaa acaaaaagcc accaaagaac cgatcaaaaa cgaagccaac
     3841 aacggcctga aaaatacccg tggtacgctg gcaatggccc gtactcaggc tccgcactct
     3901 gcaactgcac agttcttcat caacgtggtt gataacgact tcctgaactt ctctggcgaa
     3961 agcctgcaag gttggggcta ctgcgtgttt gctgaagtgg ttgaaggcat ggacgtggta
     4021 gacaaaatca aaggtgttgc aaccggtcgt agcggtatgc accaggacgt gccaaaagaa
     4081 gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt ggcgacactc tttattgcag
     4141 atcttcatct ctgcgtggaa gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg
     4201 gagaagcccg caaggccgac gcgctgtata ttcttggcga tctgtttgaa gcatggattg
     4261 gcgacgacga tcccaaccca ctccatcgcc agatggcggc agcgatcaaa gcggtgtccg
     4321 attccggcgt tccctgttat ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct
     4381 ttgcccgtga aagtggcatg acgttattgc cggaagaaaa agtgctcgaa ctttatggtc
     4441 gtcgggtgct gattatgcat ggcgacacgc tgtgcaccga tgacgcgggt tatcaggctt
     4501 ttcgcaccaa agtccacaaa ccctggctgc aaacgctatt cctcgctctg ccgttgtttg
     4561 tgcgcaaacg cattgccgtg cgaatgcgcg cgaacagtaa agaagccaac agcagtaaat
     4621 cgctggcgat catggacgtt aaccaaaatg cggtagtcag tgcgatggaa aaacatcagg
     4681 tgcaatggct gatccacggg catacccatc gcccggcggt gcatgaactt atcgccaatc
     4741 agcaacctgc ttttcgcgtg gtactgggtg cctggcatac ggaaggttcg atggtgaaag
     4801 tcacggcgga tgacgttgag ctgattcatt ttccgtttta aaaaacccgc aactttgctg
     4861 atttcacagc cacgcaaccg ttttccttgc tctctttccg tgctattctc tgtgccctct
     4921 aaagccgaga gttgtgcacc acaggagttt taagacgcat gtcttcccgc aataatccgg
     4981 cgcgtgtcgc catcgtgatg gggtccaaaa gcgactgggc taccatgcag ttcgccgccg
     5041 aaatcttcga aatcctgaat gtcccgcacc acgttgaagt ggtttctgct caccgcaccc
     5101 ccgataaact attcagcttc gccgaaagtg ccgaagagaa cggttatcag gtgattattg
     5161 cgggcgcagg cggcgcagcg cacctgccag gcatgattgc cgccaaaacg ctggtgccag
     5221 tgctgggcgt gccagtacag agcgccgcac tgagcggtgt cgatagcctc tactccatcg
     5281 tacaaatgcc acgcggcatt ccggtgggta cgctggcgat tggtaaagct ggcgcggcaa
     5341 acgcggcatt actggcagca caaattcttg cgactcatga taaagaactg caccagcgtc
     5401 tgaatgactg gcgcaaagca caaactgatg aagtgctgga aaacccggac ccgcgaggtg
     5461 cggcatgaaa caggtttgcg tcctcggtaa cgggcagtta ggccgtatgc tgcgtcaggc
     5521 aggcgaaccg ttaggcattg ctgtctggcc ggtcgggctg gacgctgaac cggcggcggt
     5581 gccttttcaa caaagcgtga ttaccgctga gatcgaacgc tggccggaaa cagcattaac
     5641 ccgcgagctg gcgcgccatc cggcctttgt gaaccgcgat gtgttcccga ttattgctga
     5701 ccgtctgact cagaagcagc ttttcgataa gctccacctg ccgactgcac cgtggcagtt
     5761 acttgccgaa cgcagcgagt ggcctgcggt gtttgagcgt ttaggtgagc tggcgattgt
     5821 taagcgtcgc actggtggct atgacggacg cggtcaatgg cgtttacgcg ccgatgaaac
     5881 cgagcagtta ccggctgaat gctacggcga atgtattgtc gagcagggca taaacttctc
     5941 tggtgaagtg tcgctggttg gcgcgcgctg ctttgatggc agcaccgtgt tttatccgct
     6001 gacgcataac ctgcatcagg acggtatttt gcgcaccagc gtcgcttttc cgcaggccaa
     6061 tgcgcagcag caagcgcaag ccgaagagat gctgtcggcg attatgcagg agctgggcta
     6121 tgtgggcgtg atggcgatgg agtgttttgt caccccgcaa ggtctgctga tcaacgaact
     6181 ggcaccgcgt gtgcataaca gcggtcactg gacacaaaac ggtgccagca tcagccagtt
     6241 tgagctacat ctgcgggcga ttaccgatct gccgttaccg caaccagtgg tgaataatcc
     6301 gtcggtgatg atcaatctga ttggtagcga tgtgaattat gactggctga aactgccgct
     6361 ggtgcatctg cactggtacg acaaagaagt ccgtccgggc cgtaaagtgg ggcatctgaa
     6421 tttgaccgac agcgacacat cgcgtctgtc cgcgacgctg gaagccttga tcccgctgct
     6481 gccgccggaa tatgccagcg gcgtgatgtg ggcgcagagt aagttctgtt aacctggtgc
     6541 tcatttgatt gccggatgcg gcgtgaacgc cttatccggc ctacaccgat ccggtaccca
     6601 ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca tggtgcgccg attgccggat
     6661 gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc cgccagcgtc tcttcaattc
     6721 gcgataacgc cccaatccac gcgggtttac cacggcttct gacataacca ctcaccgccg
     6781 ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg cgctaactca tccggtgtgg
     6841 catggcgaat ggcacgttgc tgcggcattc cccagttttc atataccgca tcagcatcgg
     6901 tgaggatcac cagtccatcg gcgttaattt gctcggcgag caacgcagcg gcgagatctt
     6961 tatcaatcac tgcttcactc cctgctccgt catccgtcac aggcacaccg ccgccgccac
     7021 tgcaaatcac cacatgcccc tctttgagca acaactcgat ggcttcgctg tcgagaattt
     7081 tacgcggttg cggagacgcc accacccggc gcaaatattt accatcacgt ttcatctgcc
     7141 agccgtaagc cgcttccagt gcctcttgtt cttctggctg ataaaccgga ccaataaatt
     7201 tctctggctg caaaaacgcc ggatcatcag gcgaaacttc aatgcgcgtc cgcaccgtcg
     7261 tgacaggcgg catctgcggc tgtgcgctca aactctgcgc cagcatatag ccaatcatcc
     7321 cctgactttc tgccaccagc acatccagcg gatacggttc tacctctttc cacgccagat
     7381 tctgcaatgc cagcagcccc acctgcggcc cgttgccgtg aacaatcgcc agccgataag
     7441 aacgggccag gcgcgccagc gcgggtacag cactggcgat attgcgatat tgattttctg
     7501 ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc aagagctaca accagtgttt
     7561 tcatggtttt ccttgtaata attgttggcc aagccagaac cccagcagtg tatctgcccc
     7621 tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca gcggtacgtt tcgggcaact
     7681 cagagcatga acaaagtgca ggagtggcga ggcgaaatat ccttgcgcgg cataacgtaa
     7741 ataactgacg ctcaccgctg tggtaacgag ttgcagattg tcggaacagg caaaaaacgg
     7801 acgaccggag cgcgcatcta aagcaccata ataccaggcc gccagcagca ttccgctcag
     7861 cgtgtcatca tgacttggcg ttaatccggg gcctttaccc agccagtgcc gccagtcggt
     7921 cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac tggcgtaact cagcaggcag
     7981 cggatcgctt gccgccaacg ccagtggccc gaaaagcccg gtttcctccg cgcgttgcat
     8041 ccatgcagct gcaagtggtt gaggatgcgc aggcggcgta atacgcagca aacaataacg
     8101 ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct tgagccacaa cctgtggtcg
     8161 ttcattgccg cataatccac cgcataacgc atcgaattgc gcacggcgaa gcacccatcc
     8221 tccggggccg aaaccactac cctgacgatg cagcgttaac agttcgccgc tttccgtcat
     8281 caggttaatc gcccgccgcc acactcccgc taaccgccag gactggcgat aattcggtgc
     8341 gctactactg gcaagcagag gatggatgat cgtcattacg cgttcctcaa ccaatcccca
     8401 tgctttcagc cagtgcttcc agcgcctgtt caaagcacgc cagcggtgcc cgcacggtgc
     8461 ctgcgccaat ctgcccgatc cccgcctctt tatgggcgat accggtattg atgagtggcg
     8521 taatgccggt ttctaccacg cgacgaatgt ccagtcccag gcacgcgccc tgaaaatccc
     8581 agcttgggat ctgcaactgc atattgcgtt caaggtaaat ttccgccatc tcttcagata
     8641 ccgccctggc cgcttccatg ccactcgcac cgacaaagcg cgttacgcca ggcgctgcga
     8701 tcattgccgc acctccgata ccaaaggttt cggtaatcgc gctatcgccc atatccgggt
     8761 tcgcctgctc ctgcgagaag ccggtgaaaa acagaccttg cggagtgttt acaggcgcag
     8821 taaaccagcg ttcgcccagc ccgcttaccc gaatcccgaa catattgccg ttgcgggtca
     8881 ttgccgtgac gatgctgcct gcgcggatca tcgcgccagc atccatcgcc gccttgcagt
     8941 aagccatcgc gaggttgagg aagaactgat cggtcacgct gaggaaatcc atcacttccg
     9001 cgatgtgctg tttatcatga tcgaggcgag caatttgtgg ggccagcgca cgcattaaca
     9061 gtgctgagga agcaatattg cgttgatgga actcatcgcc catcgtaatg ccctgcgcca
     9121 tcatcgcagt aagatcgata ccgcgctcca tgcgccccag cgccgcgctt aatactggca
     9181 ttaacacatc gcgcatccag cgatggcgag tcaggacatc ttcgccgtaa gcgccaaaac
     9241 gcatcacttt gccgatacct tcgttgaggt tgcagtacgc ccggttgccg tcggtcacgt
     9301 tctcgaccac cagcatcggc atactggcag aagtaatacc gcccattggc ccgacggcat
     9361 tcacatggtg acaaggaatg aagttcactt ccccctgctc cagtattgcc agcgcctgcg
     9421 cttcatcttt cgcccaacct tcgaacagac atgcgcccac gcacgcccct ttcatgggtc
     9481 cggtcatctc ctgccagcgc attggcggcc cggcgtgaag cagtgttttg ccctcgttta
     9541 gttcgctgat aagtgaagaa gccggttgca catccagcca gtgtggacga gcgcgacgaa
     9601 tttgttcgat aaccgcagca ttggcttgcg ccactgatgt aaacatgaga accccttatt
     9661 gcaaacgttc taataaacga gccagttttt tattgccgcc ggcgactggc gaccattggt
     9721 aatgcacaac cggtttgctg gcgctttgta gctccagcgc aaaactgcgt aatccgatgt
     9781 taatcacggc gacgttttcc agtaatgacg gtgtgtgttg ctgtgcggca ggcgagagcg
     9841 gatgaattaa cgctgccgcc agcaaggtgg cttccggtag cgaactcacg accgcaatcc
     9901 ccgcatcttc cagcgtggcg atttgctgcg agcggcattg cgggtcacgt tcagtgcctg
     9961 tcaccgtggc aatggcatac agtggttgat tgtctgaacg cgcagcacag gctttttgcc
    10021 aggcgcttac cagcgaggcg gcaggatcgg cggtcgcacc gaagccaatc acgacatcaa
    10081 gcagcagcac acgcacttgc ggtttagcgc cgagatcggt aattaactgg ttgcgtaagg
    10141 ttgggtcgat catcggatgg ggacgcccga cggtgtagaa atcatcgccg aggtcaataa
    10201 tctggtggcc atcggcgtcc agcatcatgc cgtgatgatg ggcatcatcg gcttccacgc
    10261 caaggtgtcc ggcaagtaat cccgccgctt cggcagccag cgtaccgccg gtatacaaac
    10321 cgcaaataaa tccgctgctg gcaggcatta tcgcgttacg tcgcgccgta acgcgtgaaa
    10381 gcaggcaagc caggcgtgcg gcctcatcca gcgaggaggc aaaccagaca ttctcgtcgc
    10441 gggccaccgc cggggtataa cctaaaaaca gcgccaccgt cggtttgccg gttgctttca
    10501 tggcattaac aattttcaga cgcaccgctt cggcaggtgg ttttgaaaca aatgccagca
    10561 cttcgctttt ctcgtctgca ctgagcattt ccagcgccgt cagcgcactg atgccgccca
    10621 cttcacggct gaggtcgcgg ccgccaaggc caatcgcgtg agtaattccc tcccctgcca
    10681 gcgcaatctg cgagcacagc tcctgaatcc cggtaccgga agcgccaatg acgccaatgt
    10741 tgccttccgg catcacgtta gcaaaagcca gcggcgtgcc ggcaatcatc gacgtaccgc
    10801 agtccggccc catcaccagc aagccttttt cccgcgcgcg ggttttaagt tggatttcat
    10861 cttccagcgt gacgttatcg gagaacatca tcacgttgag gttgcgatcc agcgcctgat
    10921 tcgccagctc cgccgcatac tcgccagcca ctgaaatcag cgccagattg gcatcgggta
    10981 atttctgaca ggcactgtcc cagcgacgca cctgcgtcaa cgcctggctg ctgcctgacc
    11041 cctgcgccag ttgttttagc gcctcttcaa gctgctgcat aatcgcctgc gcgatccccg
    11101 catccgccgc ttcgctacga attgccacgc aaatatcgtt cggcgtggcg ttattaaaat
    11161 cgtcatgcca gaaacctgtg gtatctaata acgctttatt ggcgggcgta cccatcatta
    11221 cggaaacatc atcaacattt tctgattcgc tgagttttcg tgaaataatc attaaactga
    11281 ccgaatcctg aaaacaccct tttttaataa aggcgtggat cataacaact ccttaaaaat
    11341 tgcacccatg gcaattgcac gagcagtgtt aatgtggcga ttacggtatc gatgacaacg
    11401 agtaaataag gtgatgaata tcacacaggg attattaaca aaagaattca aataaccgcc
    11461 atgctattac gtgttatttt ttaataagcg tggttgtttt aaaaaccagg ttaaaagcga
    11521 gcggattatc aaataaattc taactatatt tttttgcctg tctggatcac ataatccaga
    11581 tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga
    11641 tgaaaatcag tcgggaaaca ctccaccagc taattgagaa taaactctgc caggctgggt
    11701 taaaacgtga gcacgctgca accgtggctg aagtattggt ttatgccgat gccagaggga
    11761 tccactctca tggcgcggtg cgcgtggaat actacgcgga acgcatttca aaaggcggca
    11821 ccaaccgcga accggagttt cgtcttgagg aaaccgggcc gtgctcggca attttacatg
    11881 ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg
    11941 cccagcaaaa tggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct
    12001 cttattttgt gcagcaggca gcccgcgccg gattaattgg catttcgatg tgccagtccg
    12061 atccgatggt ggtgccgttt ggcggcgcgg aaatttacta cggtactaac ccactggcct
    12121 ttgccgcgcc gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg
    12181 catggggaaa agtcctcgac gcccgctcgc gtaatatgtc tatcccggat acctgggcgg
    12241 tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgcag
    12301 ccgggccgaa agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg
    12361 gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt
    12421 tggggcaatt acatgtagtt attaacccga actttttctc ctccagcgaa ttattccgtc
    12481 aacatcttag ccagaccatg cgcgaattaa atgccattac ccccgcgccc ggttttaatc
    12541 aggtttatta tcccggacag gatcaggata ttaaacaacg ccaagccgcc gtcgaaggca
    12601 tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat
    12661 acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac
    12721 attttcgtca aaccatagaa gaagcgctgc cctggctttc ctcttttggc gctgacccag
    12781 cgggtgggat gacccgttta ctttattcgc cggaatggct ggaaacccag cagcaattaa
    12841 aaaaaagaat ggccgcaagt gggctggaaa cacgtttcga tgaagtgggg aatttatacg
    12901 gtcgcctgag tggcaccgaa tatccacagg aagtgattct gagcggttcg catatcgata
    12961 ccgtggttaa cggcggtaac ctcgacgggc aattcggcgc gctggcggcg cggctggcaa
    13021 ttgactggct gaaaactcaa tacggcgcac cgctgcgtac ggttgaagtg gtggcgatgg
    13081 cggaagaaga aggtagccgc ttcccgtatg ttttctgggg cagtaaaaat atctttgggc
    13141 tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg
    13201 cgatgaaggc ttgcggattt actctgccgg acgccccgct aactccgcgt caggatatta
    13261 aagcctttgt cgaactgcat atcgaacagg gctgtgtgct ggaaagtaat gggcaatcaa
    13321 ttggcgtggt gaatgcaatt gtcgggcaac gtcgctatac ggtgacgctg aacggcgaat
    13381 caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacggtttac gctttcagtc
    13441 gcatttgcca tcagtcgatc gaaaaagcga aaaagatggg cgatccgctg gttctgacct
    13501 ttgggaaagt agagccgcgc ccgaatacgg tgaatgtggt gccgggtaaa accacgttca
    13561 ccattgattg tcgtcatacc gacgccgccg tgctgcgtga tttcacccaa cagttagaaa
    13621 acgacatgcg ggcgatttgc gatgaaatgg acattcgtat tgatatcgat ttatggatgg
    13681 acgaagaacc cgtgccgatg aataaggacc tggtcgccac cctgacagaa ttgtgtgaaa
    13741 gtgaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg
    13801 cgcctcgcgt accaacctgc atgattttta tccccagcat caacgggatc agccataacc
    13861 cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc
    13921 tttatcaact tgcctggcag aaataaggag tcataaatgg gatatttaaa taacgtcacc
    13981 ggttaccgcg atgatttact ggctaaccgt gcgattgtga aacacggtaa tttcgcactg
    14041 ttaacaccag acggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca
    14101 atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa
    14161 aacggcggca accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc
    14221 tctggaaata tcactgccaa agccgaaggc aaaacatttt ccttaagcga aggtggctat
    14281 ctttattgcc cgcctggctc cttaatgacg tttgttaacg cccaggccga agacagccaa
    14341 atctttttat ataagcgccg ctatgttccg gtagaaggct atgcaccgtg gctggtttct
    14401 ggcaatgcca gcgaactgga acgcattcac tatgaaggta tggatgatgt gatcctgctg
    14461 gatttcctgc ccaaagagtt aggttttgat atgaacatgc atatcctttc ttttgcacca
    14521 ggtgccagcc acggttatat cgaaacacat gttcaggaac acggtgccta tattctttcc
    14581 ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc
    14641 tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga agcgttcagc
    14701 tatatttact cgaaagattg taaccgcgac gtagagattt aagtcatacc actcgcctga
    14761 ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt tgcaacatag
    14821 cctgatgcgc ttcgcttatc aggcctacat gatctctgca atatatagaa tttgcgcgat
    14881 tttcaggaag gatatggtac tcatgccgca tccggcatga gtactgcact cttatgcgat
    14941 ataaatcgca tccgctttaa aggtaagggt cagtttttaa ttccctgacc tattttaatg
    15001 gcgcaggcaa tatttcgcgc gctgttgaag agattggttt caccgctggc gagcacttcg
    15061 gctaaaggtg ccagacgtgg caaaatgctg aataccgcat caatgccgta ctggtgcacc
    15121 acttccacgc catcacccaa tacgccagca atcccaatca ccggtacatt aaactgcttc
    15181 gccaccgacg caacgcccag cggcgcttta ccgcctgccg tttgcgagtc aatgcgccct
    15241 tccccggtga tcaccagtgc tgccccctgc accgcctgct ccaggttgac cgcccgcagt
    15301 acaatctcaa tacccggttt aatatccgca ttgaggaata ccgccgccgc aatccccata
    15361 ccgcccgcag cgccaccgcc cgccatctgg cagacattaa tttcagtttg ctgttgtaaa
    15421 acacgggcgt aattttgcag cccctgttca agttcttcga ccatctccgg cgttgcccct
    15481 ttttgcgggc caaacaccgc cgccgcgccg cgtgccccta ccagcgggtt atcaacgtca
    15541 catgctactt caatatggca gttcgccaga cgcggatcgc attcctccat ctcaatgctt
    15601 gccacgcgcg ctaaattgcc gccgttagcc accagcacct gaccgtcggc atcaaggaaa
    15661 cgcacaccga gcgcctgcgc catgcccata ccgccgtcga ccgtcgcact gccgccaatg
    15721 ccgagaataa tatggcgaat gccgttatcc agcgcatgac gaattaactc ccccgtacca
    15781 aaactggagg ccagcaacgg attacgcttt tcaggcgcga ccagcatcag gccacttgct
    15841 gccgccatct caatcaccgc cgttttcccg tcgccggtaa tgccataaaa agcattcact
    15901 ttttcgccca tcggcccgca gacttcaagc gtcacgatgt tgccgcccgt cgcggcgacc
    15961 atcgcatcca ccgtcccttc gccgccatcc gctatcggca aacagatata gttcgcatcg
    16021 ggaaagaggg tcgaaaaccc ggctttaatt gcctgacaac atttttctgc actcaagctc
    16081 tctttaaaag agtctggcgc aatgacaatc ttcattatca atcctttttc aacaaattat
    16141 tcgatgcgtt cccacggaat aaggttatcc ataagaacag ccagtaaaat cccgaccaat
    16201 aagccgttac tgagcagagg acgaagcgtt aatggaaggt cttgcagata cacaggcggt
    16261 aatgccatta aaaatatgcc gacaaataac ggcaatgcga gtcgataaat attgcgagcg
    16321 gtaaacgtta tttgctggct aaacaccagc gcggaaaaga gtaaaggtag ataagaaacc
    16381 agcatgaccg cactactcac gggtaaaggg atactgcaaa acagtcgcgt gagtgccgga
    16441 accagcgcca ccagcaggca aataacgctg ccataaataa acgaacgccg cgtgtaatcg
    16501 ccagtctggg ttaataaacc aatggatgaa acaaacggtg aaaatggaat tactgccagc
    16561 ggcacggtta tcagtgtcat aaatccggtc gccacaaagc tacgacgata acgcgtattc
    16621 cccgcgccct gctgcggata aaaaacatcc gtgccccgaa tcgcaccgta ggtattgctg
    16681 atattcacca gacctgtaat caccgccgtc agaataatcc ccggcgacaa agcaccgcca
    16741 ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga gcgaatgcga agaaggaaag
    16801 caaaagtacc acaacaacca gccggttatg gtgccgacca gcaggccata acgggcaaaa
    16861 cgttgcggca gaaagataat catcgccagt accaggcaca tcaccgccac cgagagcgcg
    16921 aacggcggta actgaatttt aaaattcggg tcggctatgc caaacggcag cccgagcata
    16981 cctttgaaaa agatagtggt cagctgcgcg cccagcatca acataaacaa gaccatcacc
    17041 gacggcgtaa ataaccgcgc caggcgatga cctaatccgc taaaaccaat caacatcgtc
    17101 agcacgccgg agagtgcaat ccccaccgcc aggctggtag cgatatcgtt gatcggtgtc
    17161 ccgcgcgatg cttcgccaag ggtgatagtt aggatggttc cccaccacag gccccccggc
    17221 ccttccataa tagcgcgacg atgaccgcaa aacgcctgag cgaagcaggc cagtgcggta
    17281 gcaagaaaag catattgcgt gagcgtaagc aggctacttt gcggcaactg aaaagcagaa
    17341 agtagcgtag gaggaaccac aaccgtgttg caaaaaataa agaaaaacca ctgaaatcct
    17401 gataacaggc tttcgcggct aactgcaaaa ttgaacataa acacacctta accggagaat
    17461 gcccgccgca aggacgggca ttgcaggggc cagattactg ctgatgttta aggataaatt
    17521 gacctttcgg cgcaacaggg aagccctgtt cgatgtcgta aatcacatca ccacgtaaga
    17581 tggttttcgt gatacgcgcg ccaatggtac ggccaacata cgggctgact ttgtggcgat
    17641 attccagatc gtcattggta agaacatagc tgctattcgg ctgaatgaag acgaagtcgg
    17701 catcttttcc tggggcgata cggccttttt gctgcagacc aaaaatatct gctgcgttag
    17761 tcgccattaa tttgccgaac attggcagag acattccgcg tttctgtacc gcttcatcga
    17821 acatcacgtc catacagttt tgcagaccgg caataccgcc ccatgcttcc atgatgttgc
    17881 cggctttcat ttccggaggg catggtgagt ggtcggaaac caggcggtcg atttcaccgt
    17941 taaacagttt ttcccacatg cctttctagt tttccagatc gcggatcggc ggtgaacact
    18001 tcgccagagt accgatttct tcgaactgat cggtatccag tacgaagtaa tgcgggcagg
    18061 attcacaagt aacgtcctga ccttcctgac gtgcacgagt cacttcttca acgccttccg
    18121 ggctgctgat atggcaaacg tgcagacggc aaccggcaac tttcgccaga tacagcacgc
    18181 ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc cacatagtca tgggcagtta
    18241 cgcgaccttc acgtttcgct tcttcgccca gtgcgtcaca gatcagcgcg ttttcgcagt
    18301 gcaccagcac cggctgcccc agttcgccca gcttctgcgc acctttgaag aactgccagt
    18361 cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc acaggtcgca acgaagcatt
    18421 tgaagccgac aacgcccact tcatccagct catgcagacg gtcgatgttg taagacacca
    18481 ggccaccgag ttgtgccgca tcgatagtca gcttgccttt agcggcatcg aacttcagtt
    18541 caattgaagc gcggtcaacc gttgcaggca gctggttgag cggcatttcg atcatggtgg
    18601 tgataccacc ttttgccgct gcgcgagtac cggtttcata accttcccag tggctacgac
    18661 ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg cgaaaccacc agaccagacg
    18721 catccataac ttcttttgca tcgcccagat cctgaccgat agcagcaatt ttgccgcctt
    18781 taacggcgat atctacaacg cgagcttcgt tttctaaaat aacggtgccg tttttaatga
    18841 ttaaatcaaa agacataaca aactccttgt aaaatacaga ttggttttta actcaagcaa
    18901 aggcgacgat gacgccgaca aaccatgaaa cacgcgataa cggttccatt aagggaataa
    18961 acttaccgcc aagagataaa ataacggcca ccagagttac tgaaaacgcg gtgaggttaa
    19021 aaccgttatc gtaatattta taatcgccag gtgcggtgta cagttcatca agattaattt
    19081 gcccacgcat caccacaaaa taatgcgcca tcatgacacc aattaccgga ccaagcattc
    19141 cgccgataat atcgaggaac agataaatac tgtcctgatt ttccattaat ttccacgggc
    19201 agatcagcag gctgataata ctggcaatca gtacgccgtt tttataggtc agttttgtcg
    19261 gtgcaatggc agcaatctga taaccagccg gaataatatt accagtggcg ttagtggaga
    19321 tagtcgtcat cagaataacc agtaccgcaa agaacgaggc aaacaggctg tcccaacgct
    19381 gaacaatatc cagcacgttc caggtatcag cgccgtaatg aatactggct ccggcaataa
    19441 tacatacccc cgcgaccgca aacagaatat aggccacaac taaacccagc gtttgcccca
    19501 gcgcttgctc acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc accgccggtg
    19561 ccgcccagac cgcaactacc gcgttaatca ccaccaggaa caagaatcca ctgttttctg
    19621 ctttctgaat accgctcgga atgtagtcaa agattggacc gagcccgacc agcgaaatcg
    19681 cccaaatcgc cataccgccg aaaacgatat agatgcacgg gttaagaatg gcagtgaatt
    19741 tatttaaaac tttgccaccg ccaaaaccta taccaacgtt gaccagccag aagagtaaga
    19801 aagtaattaa gcccggtaga gaaaggccta acagagtgaa atcaccaccg agagtcaaaa
    19861 atcccggcca gattttgcca atcagaatca agcaggccag tgaccccgcg taacattgca
    19921 ggccaaacca cataatggca gcaataccgc ctcttaataa tccgggaaac agtgcgccac
    19981 gtacgccgta agaagcacgc aggatcatgg caaacggtac gccgtattta ctgcccgcag
    20041 cgccgtttaa taccattacc gcggcaatga aaaaggcgct gaggataatt gccagcataa
    20101 tactaaaggt agacaagccg agaataaaaa agccgccgac catcacataa ttgggaacgt
    20161 tatgaaccga acccatccat aaggtaaaat agttaaatgt tttccaggtc cgctggcttt
    20221 gcgttttcgg caatagatct tcgctatagc cgcgttgctg gaatagtttt ctctgatgtt
    20281 ccataaatcc ctcttaattt cacgtat
//
LOCUS       DAZFKY010000079        20256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFKY010000079 DAZFKY010000000
VERSION     DAZFKY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            226..726
                     /gene="tssB"
                     /locus_tag="ACHKCL_002528"
     CDS             226..726
                     /gene="tssB"
                     /locus_tag="ACHKCL_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5763481.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            761..985
                     /locus_tag="ACHKCL_002529"
     CDS             761..985
                     /locus_tag="ACHKCL_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763482.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1036..2427
                     /gene="tssC"
                     /locus_tag="ACHKCL_002530"
     CDS             1036..2427
                     /gene="tssC"
                     /locus_tag="ACHKCL_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5763483.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2518..2931
                     /gene="tssE"
                     /locus_tag="ACHKCL_002531"
     CDS             2518..2931
                     /gene="tssE"
                     /locus_tag="ACHKCL_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5763484.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2935..4785
                     /gene="tssF"
                     /locus_tag="ACHKCL_002532"
     CDS             2935..4785
                     /gene="tssF"
                     /locus_tag="ACHKCL_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5763485.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4749..5831
                     /gene="tssG"
                     /locus_tag="ACHKCL_002533"
     CDS             4749..5831
                     /gene="tssG"
                     /locus_tag="ACHKCL_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5763486.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5856..7136
                     /gene="tagH"
                     /locus_tag="ACHKCL_002534"
     CDS             5856..7136
                     /gene="tagH"
                     /locus_tag="ACHKCL_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO5763487.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7133..7657
                     /gene="tssJ"
                     /locus_tag="ACHKCL_002535"
     CDS             7133..7657
                     /gene="tssJ"
                     /locus_tag="ACHKCL_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5763488.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7660..8991
                     /gene="tssK"
                     /locus_tag="ACHKCL_002536"
     CDS             7660..8991
                     /gene="tssK"
                     /locus_tag="ACHKCL_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5763489.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8996..9757
                     /gene="icmH"
                     /locus_tag="ACHKCL_002537"
     CDS             8996..9757
                     /gene="icmH"
                     /locus_tag="ACHKCL_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5763490.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9766..12537
                     /gene="tssH"
                     /locus_tag="ACHKCL_002538"
                     /gene_synonym="clpV"
     CDS             9766..12537
                     /gene="tssH"
                     /locus_tag="ACHKCL_002538"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5763491.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12534..13277
                     /gene="vasI"
                     /locus_tag="ACHKCL_002539"
     CDS             12534..13277
                     /gene="vasI"
                     /locus_tag="ACHKCL_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5763492.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13282..14693
                     /gene="tssA"
                     /locus_tag="ACHKCL_002540"
                     /pseudo
     CDS             13282..14693
                     /gene="tssA"
                     /locus_tag="ACHKCL_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            14830..18264
                     /gene="tssM"
                     /locus_tag="ACHKCL_002541"
     CDS             14830..18264
                     /gene="tssM"
                     /locus_tag="ACHKCL_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5763493.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18275..19684
                     /locus_tag="ACHKCL_002542"
     CDS             18275..19684
                     /locus_tag="ACHKCL_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5763494.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19650..20129
                     /locus_tag="ACHKCL_002543"
     CDS             19650..20129
                     /locus_tag="ACHKCL_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5763495.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELFYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20150..>20256
                     /locus_tag="ACHKCL_002544"
     CDS             20150..>20256
                     /locus_tag="ACHKCL_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763496.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQ"
BASE COUNT         4801 a         5539 c         5561 g         4355 t
ORIGIN      
        1 acgcgaattg cacaggttca taaaatctat ttaacgcttg cagaataccc ctctttacga
       61 tcgataaaaa gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt
      121 ggtgatttta ttttcaccgc catcaagacg gcatttccga tattttttgc ggtacgaata
      181 tgccgtgttc atggcctgta cgagataaca tgacggatca cttttatgag caaaaaattt
      241 gaaggcagcg tcgcgcctcg cgaacgtatt aatatatcgt atgtcccaaa aacagatggc
      301 cagacggctg aagtggaact tccgcttaac atgctcgttg tcggcgatac cggaaatacg
      361 caggaaacat cctcactgga tgaacgtcag gcagtttccg ttaacaagca caatttcggt
      421 gcagtgatgg ctgaagcagc cattggcctc aattttaccg tccccgccac gctgaaggga
      481 agcacaacgg acgatgaaat gaatgtggcg ctgaatatca agtcactgga tgatttttca
      541 ccagatagcg tggcccggca ggttccggaa gtgaataaat tgctggaact gcgtgaagca
      601 ctgactgcac tgaaggggcc gatgggtaac cttccggcat tccgtaccca gctccaggca
      661 ttgctggaaa atgaagagtc ccgcgagcag ttgctcaaag aaatcggcca ggtgagcaat
      721 aaataaccac cactatttca ttaaacaggg ttatccaggc atgacggcac acaaaaatat
      781 ctccgggaca taccacctct ctgttgcaga cattctgcag gtggtttacc aggtatgttt
      841 cagcccgtcc gttgaaatca accaggatgg tgtggctgcg ctgattacaa cactggatcg
      901 ccgtatcagt gatctattgg atgagattat ccatttttgt gagtttcagc agagcgcgtc
      961 gcactggcag cgcgtacttc actgacggcg aaaagccggt ttcagaaaca cagctaaaac
     1021 agaaggattt tcagtatgtc tttacaggaa gaggaactcg tttccagtca tgccgggcag
     1081 cctgagcagg aatcgtcgct gcttgatcaa atcatggcac agacccgtat tcagcccggt
     1141 tcggaaggtt atgacgttgc ccgccagggt gtgacagcct ttattgccag catcctgcaa
     1201 tccactgcat ctgccgaacc ggtcaataaa ctggccgtgg acagcatgat tgcagatatt
     1261 gatgaacgca tcagccgcca gatggatgtc atcatccatg cgcctgcctt tcagcaggtc
     1321 gagtcgttct ggcgctcact gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc
     1381 aaggtcaacg tgcttcatgt caccaagcag gaacttctgg aagactttga gtttgcgcca
     1441 gaaattattc agtccggatt ttataagcac gtttattcgt ccggtttcgg acagtttggc
     1501 ggtgaaccca tcgctgccgt gctgggcgct tatgagttca aaaataccgc gcctgacatg
     1561 aaactgctgc aatacgtcag tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc
     1621 gtttccccgg agtttatggg gctgaactcg tggaccgaac tgcctaatat caaagatctt
     1681 tatgccatct ttgaaggtcc ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat
     1741 tcgcgctatt tagggcttac ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca
     1801 gataaccctg ttaagaactt taactattac gaagatgtca gccagaacca cgaagattat
     1861 ctgtggggta atacggcctg gatgctggca tgcaatatcg cagacagttt tgccaaatac
     1921 cgctggtgtc caaacattat cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg
     1981 catctgttcg aaacgatggg gcagattcag gccaaaatcc caaccgaggt gctggtcacc
     2041 gatcgccgcg aatttgaact ggctgaagag ggttttatca cccttaccat gcgtaaagac
     2101 tctgataatg cagccttttt ctctgcaaac tcggtacaaa aaccgaagca ctttccggga
     2161 aaagatgcag aaaccaacta taagctgggt acgcagcttc cttatctctt catcatcaat
     2221 cggttagcgc actacatcaa ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg
     2281 agcgacttag aaagagagct taatacctgg atccgtcagt atgttgccga ccaggaaaac
     2341 ccgcctgcag acgtgcgcag ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat
     2401 gtagaaggcg aacccggctg gtactaggtt gcgctaagcg tgagacctca ttttaaattt
     2461 atgggggcaa attttgagct ttccctggtt ggccggttag acagggagta acacaccatg
     2521 atactggatc gggaaagaac cacaggttct ctttttgagc gtatggaagc ctcttcggcc
     2581 agaaaccggc agggaggcag tattcattcg ctcaggcaat ccattcgcca gaacctaaga
     2641 aacattctta atacccggtc cggtagttgc cggggtgcgc ctgagctggg cattgatgaa
     2701 ccggaagggg ctgaaaactt ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa
     2761 cgctatgaac cccggatctc acacgctgaa gtgcaggcag tggtttcatc cgcctccagc
     2821 ccgctggata tgacgtttca tattacagcc tgggtgacat ttaacgaaac gcatgaggtg
     2881 cttgaatttg atatggcacc gaatggcagc cagcattacc gggtggactg acaaatggaa
     2941 tttgaagaac gctattttcg ggaagaactc gattacctgc gccagcttag caagctgctg
     3001 gcaacggaaa aaccccatct ggcccgcttc ctggccgaaa aagatgcgga tccggatatt
     3061 gaacgcctgc tggaaggggt ggcttttctt accggcaatc tccgccagaa aattgaggat
     3121 gaattcccag aactgacgca cgggcttatt aagatgctat ggcctaatta cctgcgtccg
     3181 gttccggcaa tgacccttat tgaatatacg ccggatatgg ataagtcttc tgtaccggtg
     3241 ttaatccccc gtaatgagca gtttacaacc aacgccgggg aaatcagagt tgatgaagtg
     3301 ctgccctctg atgctaaaaa ggaggagccg cctccctgta ccttcaccct ctgccgggat
     3361 atctggctgc tgcccgttcg cctggagcag attgaaaacc gcagtacgac ccgtaatggt
     3421 gttatcaaca tcaccttttc ggtcgcaccg ggaacggact tccgcacgct ggatctgaac
     3481 aaacttcgct tctggctcgg caatgacgac aactataccc gtgaccagct ttatttatgg
     3541 ttctgcgaat acttgcaggg tgccgacctg actgtgggtg aacagcatat tcgcctgcct
     3601 gagtttatgc taaaagctgt cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa
     3661 aacgtccaca gcggctaccg gatccttcag gagtatttct gttaccccga tgcgtttctc
     3721 ttttttgatc tttgtggttg tccggctttg cctgacggat tgcaggcgga attctttacc
     3781 ctgcaactgc gtttttcgcg ccctttgccc gtggacatcc ggctgcgccg cgattccctg
     3841 cgcctgtatt gcgcacctgc cattaattta tttatccacc atgcagaagc catcacgctg
     3901 gacaaccggc gggcagacta tccgctggtt cccagccgcc attacccaca acattacgat
     3961 gtattttccg ttaacagtgt ggtgagccag gtccaggata tgttcaggaa aaaagatctg
     4021 gggcgtcctg tttcgacgca ggccgcgcgc cagtggccag cctttgaaag tttcagccat
     4081 cagatggaat acagccggaa gcgggaggtg gtgtactggc atcaccggac caaaacatcc
     4141 ctgttccatc gcggctttga tcataccctt gcctttatac atgctgatgg cagttatccg
     4201 tcagacgaat ctctgctcag taatgaagtg gtttcggtat cgctgacctg taccaaccgt
     4261 gagcttccgt cacaaattcg ttccggcgat atcaccggca caaccggtaa aaatgcggct
     4321 gttgcttcct ttcgcaacat tacccgcccg acgcaaccac tctggccggt cattgatggc
     4381 agcctgcact ggtccctgct ctccgccatg aacctgaatt atctgtcatt actggatacg
     4441 gacgcgctga agcaggtcat cgccaacttt gatcgccacg caatccacca tccgcagacg
     4501 gcgcggctgt cacaacaaaa gctggatgcc attgagcgtc tggagacccg ccccgttgat
     4561 cgcctgttta cgggtattcc cgtccgggga ctggcctcca cgctgtatct gcacccggag
     4621 ccgtttgtct gtgaagggga aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg
     4681 ctgtacgcca gcgttaactc attccacatg ctgaccgttg tgaacacaga aagccaggag
     4741 acatggaaat ggacggaaag aatcgggcag catcctctta tctgagtccg ggaaatcccc
     4801 cggcagataa agagcagaat gatccgctgg cacaggtttt ccataatgcc tgctcttaca
     4861 atttttttgc gatggcggag ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag
     4921 aattatccct gcgcgacgat ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc
     4981 ttgccttccc gtgcagtgat atcagtgcgc tgaaacggga tacgtcaggg gcattcagga
     5041 tgaccactac ctttatgggg ttacagggga gccagtcgcc tctccccggt tattacctcg
     5101 atcacctggc ctggaaagcg gtacatgaac aaagtccggt aggtgatttt ctggatatgt
     5161 tcagccaccg cctgacccag tttgtctggc atatctggcg taagtaccgc tatcacatca
     5221 gttttcgtaa cggcggtgtg gatgcgttct cgcagcgcat gtactccctt gtcgggctgg
     5281 gccaccgcca gcttcgcgat aagctggcga ttaatcacag caaaatgctg gcgtattccg
     5341 ggatcctggc aaatccggga cgatccccgg aaattatctg tggcctggtg tcccactgct
     5401 ttgatttgtc agaagtgacg ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg
     5461 atcagcaaaa cagcctgggg agctattctc ttaaaaatgg cgaaaaactg gcgggaagat
     5521 cggtactggg caactttgtt ctgggtacac gagtacccga tctcagcgga aaattccagc
     5581 tcagtattac cagcctgacc cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact
     5641 tcctgccact gacgacgttc gtgtccttca tcctgcgcga tcagcttgcc tgggacttac
     5701 atctggggct ggcgccggag caggtgggcg caatgcgcct gggcgataac aaaagtgcgc
     5761 tgctgggctg gaccagcttc ctcggcacac cggaagaacg accctcagtc accatcaggg
     5821 tacggtcgta aattatgata atcacggagc agaatatgcc ggaagagaaa ctacagacgc
     5881 tttcattgca ggtcattaac ggcagtgagc tggaaagcgg gcgggcggcg cgctgtctgt
     5941 tcacacagca gggaaatgtc ggccatgccc ccgaatgcca ctggtcggta caggatcgtc
     6001 agcagagcat tccggcccag gcttttaccg ttatcctgca cgatggcaca ttttgtctac
     6061 gcccgcagac ggcacaactg tggctgaatc aggcaaaagt cacagcaaca tcagacctga
     6121 tacagttgcg ccagggcgat gagatccaga tcggacggct gatggtgagg gttcatctga
     6181 accggggaga tattccccat tacgatgagg aaatggccac tcccgaaacc atcgttacca
     6241 atcgcgatat gctcacggat accctgctat caacggaggg tgcgccacac tatccgggaa
     6301 tgactcaccg gcaccagctt gcagacaccg tggtaaatgg tttttctgcc gatccactcc
     6361 aggcacttca gtccgaaagc ctgattacca cgggcgatcc gctttcaggc attgcggctg
     6421 tccggccatc ggcaccgctg tccgatccgg caagtaatgg ggggatcaat actccgttta
     6481 tggatctgcc gcccatttat gccagccctg gcgatcataa tgatgacatc tctgcggcag
     6541 aaatggcgca acgccacctt gcggtcaccc ccttactgcg cggtcttggc ggctcgctta
     6601 ccgtgagcaa ttccgacgat gcggatgatt ttctggagga ggccggacga acgttacagg
     6661 ccgcaataaa aggtctgctc gatttgcagc agcagcgtaa cagcctctca gacaaacatt
     6721 tgcgcccgct ggaagataac ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg
     6781 tgatgtttgc cgaaggtaaa agcccggtac atctggcggc tcccgccgcc gtcagtgaaa
     6841 gcctgcgcaa tgtccgccac cacgaagaag ctaaccgggc ggcgattgtg gagtcgcttc
     6901 gtgtcctgct ggatgctttc tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc
     6961 gcagccatga actgcgccag ccgctggatg atgccggagc atggcaaatg tacagccatt
     7021 attacgaaga actggcctcc gatcgccagc aggggtttga gatgctgttt aacgaggtct
     7081 acgcccaggt ctatgaccgg gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag
     7141 gaaggcgttt ctggcctgcg tgctgatgtg cgtcatatta accggctgcg aaacggcgaa
     7201 aaaaatcagc gaggtgatca aaaacccgga tatccaggtc gggagcctga aggagcaacc
     7261 ttcggaaata accgtcaccc tgctgactga accggacacc aataccaatg cggagggtga
     7321 atcagccgcc gtggatgtgc aactggtgta cctgactgac gactcaaaat tgcaggcagc
     7381 cgactatgac cagatagcca gtacgccgct accggatgtg ctgggtaaaa actatatcga
     7441 ccaccaggat ttcaacctgt tgccggacac catcaaaacc ctgccgccga taaagctgga
     7501 tgagaaaacg cagtttattg gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg
     7561 gaaacaaatc gagacggtgg aaggaaccgg ccaccattac cgcctgctgg tgcatgtacg
     7621 tcaaagcagc atcgaaatga aaaaagagga tgaataatta tggcaaccac ccgcaacaag
     7681 gtgatgtggc aggaagggat gctgatgcgc ccacaccatt tccagcaaca gcagcgttac
     7741 aacgactacc tggataacca gcgtttccgg gccatgaatg atttatcctg gggatttacc
     7801 gaactcaccc tcaacaatga actgctggcg cagggtaaga tcatgattga cagcgcgtca
     7861 ggcacactgc ctgacggcac cgtcttttct atccccgacc aggacgcact gcccgatccg
     7921 ctgcacccgc aaaattttcc ggacgaaaga agccgcaata tttacctcgc tctgccggtc
     7981 gccagtgatg tgagaaatga aatcagcgac gggcggcgaa tcgggcgtta ccggctgaat
     8041 tatgccgatg tccgggatct gcattcagaa gaaggcgacg cgcgaacact gacgctggga
     8101 caactgacgc cgcgcattat gagcggtgca gaagatatga gtgcctatat tacgctcccg
     8161 ctttgccgta tcagcgatcg ccatgccgac ggctccctga cgctggatga cgattttatc
     8221 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
     8281 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
     8341 ggcatcgcgg atgtggcgga atttatgatg ttgcagttgc ttaaccgtaa ccagacccgg
     8401 tttacccatc gcgcccgccg atcccagctc cacccggaag atttctacct tgatcttgcc
     8461 gagttgctgg gtgaactgat gacctttaca gagccgtcac gcctgccctg cccgcttgat
     8521 gtgtatgatc atcatgacct gaccaaaaca tttaaaacac tgttaccgga agtcaaacgg
     8581 gcgctgcata ccgtactgtc gccaagagcg gtcaatctgc cgctgcatct gcgtgacggc
     8641 atctggcagg ccgatgttca tgactcggaa ctgctacagt ctgccacctt tgtactggcc
     8701 gtggcagcaa atatgcctgt cgatcagatc cagcgtcagt ttatccagca gtcgaaaatt
     8761 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tcccgggtat tcctctgcgc
     8821 gcactgatgg tggccccccg ccagcttccc taccattccg ggttcagcta tttcgaactc
     8881 gacaagagcg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgta
     8941 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
     9001 tgaggggtca ctgtctctgc caccatttac cggatacgac gaaaaaagcc tgcgcgacta
     9061 ccatctcgcg cttcatggca acagcctgaa tccgatgatc gacgcggcga cgccgctact
     9121 cggtatggta atgcgccttt ccaccatgaa cagccagacg atgccggagc atctttttgc
     9181 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggaacagg gctacgaacc
     9241 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
     9301 taacggctgg tcgaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
     9361 cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
     9421 acgttaccag gatctgctgg aatttctctg gctctgtttt tctctgggat ttcgcgggcg
     9481 ctacaaagtg gcagtacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
     9541 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
     9601 ccaaggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
     9661 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
     9721 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
     9781 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
     9841 tcagaatgta tgagccagca ggcagcggaa attaccgtca gccatgtgct tattcagatg
     9901 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
     9961 gagttgcgcc aggcgttgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    10021 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctggctgct ggcgtcggct
    10081 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccttgct gcattcgccg
    10141 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    10201 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    10261 ggtaaaggcg caggcacact gacggacgcc tctgacaccc tgcttgcccg ctacgccaaa
    10321 aacatgacag aagacgcccg taacggcagg ctagccccag tactgtgccg cgaccacgaa
    10381 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc
    10441 gaagcaggcg tgggtaaaag cgcgctgatt gaagggctgg cgctgcgcat cgtggcaggc
    10501 caggtgccgg acaaactgaa aaacaccgat atcatgaccc ttgacctggg cgcattgcag
    10561 gccggggcat cggtgaaggg tgagtttgaa aaacgcttca aggggctgat ggcggaggtc
    10621 atttcctctc cggtgccggt cattctgttt atcgacgaag cgcataccct gattggcgcg
    10681 ggcaaccagc agggcgggct ggatatctcc aacctgctga aaccggcgct ggcgcgtggc
    10741 gaactgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgagaaagac
    10801 gccgccctgt cgcgccgctt ccagttagtg aaggtcagcg aacccaacgc tgccgaagcc
    10861 accattattc tgcgtggtct gtcggcggtc tatgaacggt ctcacggcgt gctgattgac
    10921 gatgatgcct tgcaggccgc agcaacgctg agcgaacgtt atctctccgg gcgtcagtta
    10981 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    11041 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    11101 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    11161 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcttggcac
    11221 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    11281 gaggacgatg cggcgccgtt gccggacgca gatactgtgg aggatacgca gccagagtca
    11341 gagtcagagt cagaacagga taataccggt gccgtaccgg ctgatgagac cgacagagaa
    11401 cagccggaag agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc
    11461 gaactggacg ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag
    11521 attgcggcgg tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa
    11581 atgtcggtca tcaccgacct gccgaaatgg ctgggcgaca ccatcaaagg ccaggacctg
    11641 gcgattgcca gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga
    11701 cgcccgctcg gtgcgttcct gctcgctggc cccagcggcg tgggtaaaac cgaaaccgtc
    11761 ctgcaactgg cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc
    11821 gagttccagg agaaacacac cgtttcgcgg ctgattggct cccctccggg ctatgtcggc
    11881 tatggcgaag gcggcgtgct gactgaagcc atccgccaga aaccctactc ggtggtgctg
    11941 ctcgatgaag tggaaaaagc gcacccggat gtcctcaacc tgttctacca ggcgtttgac
    12001 aagggcgaga tggccgacgg cgaaggccgc ctgattgact gtaaaaacat cgtcttcttc
    12061 ctcacctcca acctcggtta ccaggtgatt gtcgaacacg cggatgaccc ggaaaccatg
    12121 caggaagccc tctatccggt gctggcggac ttctttaaac ctgccctgct ggcgcgaatg
    12181 gaggtggtgc cgtacctgcc gctgtcgaaa gagacgctcg ccaccattat cgccgggaaa
    12241 ctggcccgtc tggataacgt gctgcgcagt cgctttggtg cagaagtggt cattgaaccg
    12301 gaagtgacgg acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg
    12361 gaatctgtca tcgatggcga catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg
    12421 gcggctaaca cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca
    12481 gacgtggaag atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca
    12541 ggttgcgcgt tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg
    12601 ccactgccgc gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca
    12661 cgaccccgcc ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg
    12721 cggcgctgga gcggcttgac tgctatgacc acctgctggc accgctgtcc ccgtcagggt
    12781 ttgacggggc gctggtcaaa gccggttttg tgggcgaggc ctggacccgc gccacagagc
    12841 aggaaaaacg ccgcgagggc aacaccacag aactgctggt gacgcaggtg ccgggggagc
    12901 gcccgacggt ggtgataacc accccggcta tcggccatgt tccgccgcgc ccggtgctga
    12961 tgttcagttg cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg
    13021 tccatgatat cgccgtcact ctgaatgccg acagccgggc attgcgcagc cactggtttg
    13081 tgcgcgagaa cggcaccctg ctggagtcca gccgggggct gtccgggatt gatgaaatta
    13141 agcaactgtt cggcgcaaaa acgctgacag tggataccgg cgcagataac gccgcaggaa
    13201 agctgacctt taacattgac gggctggcaa gggcgattgc ccccctgcgc gatgcctgcc
    13261 actgggcggg agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg
    13321 cacctgctgg aaaacctgcc ggaagaaaaa ctggcatcgg cgctgaaaga tgacaacccg
    13381 gactgggagt atatcgacgg cgaaatcgtc aagctggggt ctctggccca cgctcagctt
    13441 gatattcccg aactacaacg cagggggcta cagcttctgg cttctgaaag caaagacttc
    13501 aggctactgg cacacctgct cagaaccctg caacatgccg gtgatccact gctggcactg
    13561 cacctgctaa cgctatacgt ggaacattac tggactgtgg ccgcgccgca gaatatggcg
    13621 cataaaaaac gctttgccag ccagatcatt aaacgttttg aaacggtatt gaaggctttt
    13681 cacaaaacgc tgccacaacg cagcgcgata ctctgctggg tgagctggcg aaactggcgc
    13741 agtgctggca gtcacataac gtcccggaac tggcacaggc taccgatgat ctttttgccc
    13801 tgtaccagcg tacgtttaat cgtgcggctc ctgctccggt ccccactccg gcggcctccg
    13861 gtagttcacc acaaaccacc gtcacgtctg aaagcggcgt gacgcaaccc agtgctccgg
    13921 ctccccaaat cgccatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag
    13981 tggcggctat tttatgtgaa cgccagccgg actcgccgca gggctatcgc ctgcgccgcc
    14041 atgccctgtg gcaatccatc accagtacac cccaggcgga aagcgatgga cgtaccccac
    14101 tggctgcggt ctctgccgat atggtggcgg attaccagtc ccggcttgcc agcgcggata
    14161 tggcgctgtg gcaacaggtt gagaaaagcg tattgctggc tccttactgg ctggacggtc
    14221 actgtctttc tgcacagacg gcactgcgtc tgggttacaa acaggtggca gacaccatcc
    14281 gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg ctgtttaatg
    14341 accgcacacc gtttctcagt gagcagacga aacaatggct ggctgcttcg cccgacggca
    14401 aagttgcacc ggttgcgcaa atcggtgagg aatcgcaggc agccagagcc tgttttgctg
    14461 ggcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc gatccccgcg
    14521 atcagtttca ccgccagtac cttgccgcac agttgacgga ggaggcgggg ctgatacagc
    14581 ttgcgcagca acagtaccgg atgttgttga tgatagggag tcagatgatg gtgtctgact
    14641 gggagccatc attacttacg cagcttgaac aaaaattcac ggcagaacaa taaaagagaa
    14701 caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc aaatcagccc
    14761 tgcgaccggc gatgccccgg tttaaagtct ccgctttctg gctgctgata ctggcgtgga
    14821 tttttctgct tgctgatact ggcgtggatt tttctgctgg tatggatctg gtggaaaggc
    14881 ccgacgtgga cgctgtatga agaacagtgg ctcaaaccac tggcgaaccg ctggctggca
    14941 acggcggcgt gggggattat tgccctggtg tggcttaccg tccgggtaat gaagcgcctg
    15001 caactgcttg aaaaacagca aaaacagcag cgcgaagagg ccattgatcc gctcagtgtg
    15061 gagctgaacg cccagcagcg ttaccttgac cgctggctgc tgcgcctgca acgccatctc
    15121 gacaaccgcc gtttcctgtg gcagttaccg tggtatatgg tcatcggccc ggcgggcagt
    15181 ggcaaaacga cactgctgcg cgaggggttt ccgtcagaca ttatttatgc cccggagggc
    15241 gcgcgcggca cagaacaacg cctgtacctt acgccccatg tcggtaaaca ggcggtgatc
    15301 tttgatatcg acggcacact ctgcgctccc gctgatgcgg atatcctgca tcgccgtctg
    15361 tgggaacatg ccctcggctg gctgaaagaa aagcgcgcgc gccagccgct gaacgggatt
    15421 attctgacac tcgatttacc cgatctgctg accgcagaca aacgccaccg cgagcatctg
    15481 ttacagacgc tgcgcagccg cttacaggat atacgccagc atcttcactg ccagttaccg
    15541 gtttacgtgg tactgacccg gcttgattta ttacagggtt ttgccgccct gttccagtcc
    15601 ctgaacagac aggatcgcga tgcgattctg ggcgtcacgt tcacccgccg tgcccatgaa
    15661 aatgatgact ggcgaacaga gttgaatgct ttctggcaga catgggtgga tcgaatgaat
    15721 ctggcgttgc cggatctgat ggtcacccag actcacaccc gcgcgtcttt attcagtttt
    15781 tcccgccaga tgcagggaag ccgtgaaccg ctggtgtcac tgcttgaggg tctgcttgac
    15841 ggtgaaaata tgaacgtgat gctgcgtggt gtctatctca cctcttcgct tcagcgcggg
    15901 cagatggatg atatattcac ccagtctgcc gcccgccagt accgactggg caataaccca
    15961 ctggcgtcct ggcctctggt ggacaccgcg ccttatttca cccgcagctt gttcccgcag
    16021 gcattactcg cagagcctaa tctggcaacg gagagccgcg cctggctgat gcgttcccgt
    16081 cgccgcctga cggttttctc tgccaccggc ggcgtggcgg cactgctgct catcaccggc
    16141 tggcatcact attacaacgg taactatcag tccggcatca ccgtgcttaa gcaggccaaa
    16201 gcctttatgg atgtgccgcc tccacagggg gaagatgact acggcaacct gcaactgccg
    16261 ctgctgaacc cggtacgcga tgccacactg gcctatggcg actggggcga ccgcagccgt
    16321 ctggccgata tgggactgta ccagggacga cgtatcggac cttatgtgga acagacctat
    16381 ctgcaactgc tggagcaacg ttacctgccc tcgctgttta acgggctggt taaagcgatg
    16441 aatgccgcgc cgccggagag tgaagaaaaa ctcgcggtgc tgcgcgtgat gcgaatgctg
    16501 gaggacaaaa gcggacgtaa caatgaggtg gtgaagcagt atatggcaaa acgctggagc
    16561 gaaaagttcc acggccagcg cgatatccag gcacaactga tgtcccatct tgactacgcg
    16621 ctggctcata ctgactggca cgcagagcgt caggcgggcg acggtgacgc catcagccgc
    16681 tggacgccat atgacaagcc cgtggtatca gcacagaaag aactgagcaa actgcctgtc
    16741 taccagcggg tttaccagag cctgaaaacg cgggcgctgg gcgttcttcc tgccgacctc
    16801 aatctgcgtg accaggtagg gccaaccttt gaccaggtgt ttacatctgc cgatgacaac
    16861 aaactggttg ttccacagtt tcttacccgt tacggcctgc aaagctattt tgtaaaacag
    16921 cgcgatgaac tggttgaact gacggcgatg gattcctggg tacttaacct tacccgcagc
    16981 gtgaaataca gtgacgccga ccgcgcggaa atccagcgcc agctcaccga gcagtatatc
    17041 agcgactaca ccgccacctg gcgggccggg atggacaatc tgaatatccg caattttgag
    17101 tccatcggac aactgaccgg ggcgctggag caggttatca gcggcgacca gcctttgcag
    17161 cgggcgctga ccgtgctgcg tgacaacaca cagccaggcg tcttttctga aaaactctct
    17221 gccaaagaac gggatgaggc cctggcagag ccggactacc agttactcac ccgtctcggg
    17281 catgagttcg ccccggaaaa cattaccctg gcagtacaga aagacaaaga aagcacgatg
    17341 caggccgtgt atcagcaact caccgagttg caccgctacc tgctggcaat ccagaacgcg
    17401 cctgtaccag ggaaatcggc gctgaaagcc gtgcagttac ggcttgatca gaacagcagc
    17461 gatccgatat tcgccacccg ccagatggca aaaacgctgc ctgctccgct caaccgctgg
    17521 gttggcagac tgactgacca ggcctggcat gtggtgatgg tggaggctgt acattatatg
    17581 gaagtggact ggcgcgacag cgtggtgaaa ccgtttaacg agcaactggc aaataactat
    17641 ccgtttaatc cgcgttctgc acaggatgcc tcactggatg ccttcgaacg cttctttaaa
    17701 ccggatggca tactggatac cttctaccag cagaacctga agctgtttat cgataatgac
    17761 ctgagtctgg aggatggcga taacaacgtc attattcgcg aagatattat tgcgcaactg
    17821 gaaactgcgc agaaaatccg tgacatcttc ttcagcaaac agaacggtct gggaacatcc
    17881 tttgccgtgg aaacggtatc gctttcaggc aataaacgcc gcagtgtact gaaccttgac
    17941 ggtcagttag tcgattacag ccagggccgt aactataccg cccatctggt ctggcctaac
    18001 aacatgcgcg aaggcaacga aagtaagctg acgctcatcg gcaccagcgg caacgcgccg
    18061 cgcagtatca gcttcagcgg gccgtgggcg cagttccgcc tgttcggggc cggacaactg
    18121 accggagtac aggatggcaa ctttaccgtg cgctttagcg tggacggtgg cgcgatgacc
    18181 taccgtgtgc ataccgacac ggaagataac ccgttcagcg gtgggttgtt cagccagttt
    18241 ggtctgtcag acacactgta ctgaggaggc agggatgaat agtaacgtac tgacacaaac
    18301 tatcgttacc ggcagtgacc cgcgcgggct gccggaattc agcgccatcc gcgaggaaat
    18361 aaacaaagcc agccacccgt cacagcctga gctgaactgg aaactggtgg agtcgctggc
    18421 gctggcgatt tttaaagcca acggtgtgga tttacacacc gccacctact atacgcttgc
    18481 ccggacacgg acacagggac tggcgggatt ctgcgaaggt gcggaactgc tggcggcaat
    18541 ggtaagccac gactgggata agttctggcc gcagggcggc ccggcgcgta ctgaaatgct
    18601 ggactggttt aactcccgaa ccggcaatat tctgcgtcag caaatctcct ttgcggaatc
    18661 cgacctacca ctgatctacc gcacagagcg ggcattgcag cttatctgcg ataagctcca
    18721 gcaggtggaa ctgaagcgcg ttccgcgcgt ggagaatctg ctctatttta tgcagaacac
    18781 gcgtaaacgg cttgaacccc agctgaagag taacactgag aacgccgcac agaccacggt
    18841 cagaacgctg atttatgccc cggaaacaca ggcatcttcc acaccagaag cggtagtgcc
    18901 tcccctgccc ggcctgcctg agatgaaagt ggaagtgcgc agtctgacag agaatccccc
    18961 acaggccagt gtgataaagc aaggcagtac ggtaagaggg tttatcgcag ggatcgcctg
    19021 ttcagtggct gtcgcctcag cattgtggtg gtggcaggtc tatccggtgc agcagcaact
    19081 gttacaggtt aacgacaccg ctcagggcgc agcaacggtg tggatggcct cacctgaact
    19141 cgaaaactat gagcgcaggc tgcaacaact tcttgatacc tccccggtac agccgctgga
    19201 aaccgggatg cagatgatgc gtgttgccga cagtcgctgg ccggaaagcc tgcaacagca
    19261 acaggcctcg acacaatgga atgaggcact caaaacccgc gcacagagta gcccgcagtt
    19321 gcgtggctgg ttgcagaccc gccaggactt acatgctttt gcagatctag tgatgcagcg
    19381 cgagaaagag ggactaaccc tttcctatat caaaaatgtc atctggcagg cggagcgggg
    19441 actggggcag gaaacacccg ttgagtctct gttgacgcag taccaggatg cccgtgcgca
    19501 gaagcagaat acagatgcgc tggaaaaaca aattaatgag cgactcgaag gcgtgttaag
    19561 ccgctggctg ctgctgaaga ataatacgat accgacgata aaaaaagcat tgaatttcaa
    19621 taacattcat gaatacaaag gagttttaaa tggcgaattt aatttattta acactaaatg
    19681 gtgataatca agggctcatt tcaagtggtt gttcatcaca accatccatt ggaaataaag
    19741 ctcaaacagc acacattgat caaattatga tttatgaatt catgcatgga ttaaatcgag
    19801 atcaaaacgt caaccatcat catttgacaa taaaaaaacc tatagataaa gcatcaccct
    19861 tattaggcaa agcaatttgc gacaatgaat tattgacatg tgatttttcc ttttatcgta
    19921 caaataaatt tggaattaat gaactgttct ataaaatcaa attaactgga gcaaagataa
    19981 gcgacattca tgtctctata tcacatattg ttgttgataa cagcgttcaa cccgaagagt
    20041 cagtttcttt cagttatgag tcaattatat gggagcattg ttccgctggc actagtgcat
    20101 acagtttatg ggaagatcgt cttttttaaa aacatatggg attattataa tggctaacaa
    20161 tattttacac tcttcaacat cagacagtga aagcaaaaat acaagcactg acagtacgtt
    20221 atttaatgaa aaattcccat ttcaactaag acaaga
//
LOCUS       DAZFKY010000080        20050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFKY010000080 DAZFKY010000000
VERSION     DAZFKY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..458
                     /gene="yhaJ"
                     /locus_tag="ACHKCL_002545"
     CDS             <1..458
                     /gene="yhaJ"
                     /locus_tag="ACHKCL_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5763497.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(509..865)
                     /gene="yhaI"
                     /locus_tag="ACHKCL_002546"
     CDS             complement(509..865)
                     /gene="yhaI"
                     /locus_tag="ACHKCL_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5763498.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(1106..1471)
                     /gene="yhaH"
                     /locus_tag="ACHKCL_002547"
     CDS             complement(1106..1471)
                     /gene="yhaH"
                     /locus_tag="ACHKCL_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5763499.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(1763..2749)
                     /gene="yqjG"
                     /locus_tag="ACHKCL_002548"
     CDS             complement(1763..2749)
                     /gene="yqjG"
                     /locus_tag="ACHKCL_002548"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5763500.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDARLG"
     gene            complement(2819..3211)
                     /gene="yqjF"
                     /locus_tag="ACHKCL_002549"
     CDS             complement(2819..3211)
                     /gene="yqjF"
                     /locus_tag="ACHKCL_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5763501.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(3397..3696)
                     /gene="yqjK"
                     /locus_tag="ACHKCL_002550"
     CDS             complement(3397..3696)
                     /gene="yqjK"
                     /locus_tag="ACHKCL_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5763502.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(3686..4090)
                     /gene="yqjE"
                     /locus_tag="ACHKCL_002551"
     CDS             complement(3686..4090)
                     /gene="yqjE"
                     /locus_tag="ACHKCL_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5763503.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(4093..4398)
                     /gene="yqjD"
                     /locus_tag="ACHKCL_002552"
     CDS             complement(4093..4398)
                     /gene="yqjD"
                     /locus_tag="ACHKCL_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5763504.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(4436..4804)
                     /gene="yqjC"
                     /locus_tag="ACHKCL_002553"
     CDS             complement(4436..4804)
                     /gene="yqjC"
                     /locus_tag="ACHKCL_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5763505.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4951..5334)
                     /gene="mzrA"
                     /locus_tag="ACHKCL_002554"
     CDS             complement(4951..5334)
                     /gene="mzrA"
                     /locus_tag="ACHKCL_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5763506.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(5338..6000)
                     /gene="yqjA"
                     /locus_tag="ACHKCL_002555"
     CDS             complement(5338..6000)
                     /gene="yqjA"
                     /locus_tag="ACHKCL_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5763507.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(6022..6105)
                     /gene="yqiM"
                     /locus_tag="ACHKCL_002556"
     CDS             complement(6022..6105)
                     /gene="yqiM"
                     /locus_tag="ACHKCL_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO5763508.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(6345..7121)
                     /gene="exuR"
                     /locus_tag="ACHKCL_002557"
     CDS             complement(6345..7121)
                     /gene="exuR"
                     /locus_tag="ACHKCL_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5763509.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(7251..8549)
                     /gene="exuT"
                     /locus_tag="ACHKCL_002558"
     CDS             complement(7251..8549)
                     /gene="exuT"
                     /locus_tag="ACHKCL_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5763510.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            9032..10444
                     /gene="uxaC"
                     /locus_tag="ACHKCL_002559"
     CDS             9032..10444
                     /gene="uxaC"
                     /locus_tag="ACHKCL_002559"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5763511.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            10459..11946
                     /gene="uxaA"
                     /locus_tag="ACHKCL_002560"
     CDS             10459..11946
                     /gene="uxaA"
                     /locus_tag="ACHKCL_002560"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5763512.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            12029..12580
                     /gene="ygjV"
                     /locus_tag="ACHKCL_002561"
     CDS             12029..12580
                     /gene="ygjV"
                     /locus_tag="ACHKCL_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5763513.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(12585..13829)
                     /gene="sstT"
                     /locus_tag="ACHKCL_002562"
     CDS             complement(12585..13829)
                     /gene="sstT"
                     /locus_tag="ACHKCL_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5763514.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(14228..15193)
                     /gene="alx"
                     /locus_tag="ACHKCL_002563"
     CDS             complement(14228..15193)
                     /gene="alx"
                     /locus_tag="ACHKCL_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5763515.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(15477..16463)
                     /gene="ygjR"
                     /locus_tag="ACHKCL_002564"
     CDS             complement(15477..16463)
                     /gene="ygjR"
                     /locus_tag="ACHKCL_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5763516.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(16542..17234)
                     /gene="ygjQ"
                     /locus_tag="ACHKCL_002565"
     CDS             complement(16542..17234)
                     /gene="ygjQ"
                     /locus_tag="ACHKCL_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5763517.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(17311..17814)
                     /gene="ygjP"
                     /locus_tag="ACHKCL_002566"
     CDS             complement(17311..17814)
                     /gene="ygjP"
                     /locus_tag="ACHKCL_002566"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5763518.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17899..19035
                     /gene="rlmG"
                     /locus_tag="ACHKCL_002567"
     CDS             17899..19035
                     /gene="rlmG"
                     /locus_tag="ACHKCL_002567"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5763519.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            19319..19633
                     /gene="higB"
                     /locus_tag="ACHKCL_002568"
     CDS             19319..19633
                     /gene="higB"
                     /locus_tag="ACHKCL_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO5763520.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            19630..20046
                     /gene="higA"
                     /locus_tag="ACHKCL_002569"
     CDS             19630..20046
                     /gene="higA"
                     /locus_tag="ACHKCL_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5763521.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         5185 a         5125 c         5070 g         4670 t
ORIGIN      
        1 atattgttat cgcgccggat atgcattttc gttcctcgtc ggagatcaac tcgcgcaagc
       61 tctatacgtt aatgaacgtc tacgttgccg cgcctgatca cccgattcat caggagccgg
      121 aaccgttatc tgaagtgacg cgtgtgaaat atcgtggaat tgcggtggcg gataccgctc
      181 gtgagcgccc ggtgttgact gtacagctgc tggacaaaca gccgcgctta acggtgagca
      241 cgattgaaga taaacgtcag gcattactgg cggggcttgg cgtggcgacg atgccgtatc
      301 cgatggtcga aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg gaatcgacca
      361 gcgagatcga tattattatg gcctggcgtc gtgacagtat gggggaagcg aaatcctggt
      421 gtctgcggga aattcccaaa ctttttaacg gaaaataata tttattattg ttaataaaaa
      481 ggggcgaaat atcgccccaa ttctaagatt aatttgaacc aaacttcgga tcgtttccgt
      541 aacgattgct gccagaagta ccttctgtgc agaaaaaaac cagaagcacg agccagccaa
      601 taaacggaac aaaaaagagc aaggcccagg cacctgaacg gtcggtatcg tgtaagcgac
      661 ggatagcaag cgcaaggacg ggaaggaagg ttgccagcaa ataaagcata gacagatatg
      721 gaagctccag acctaaaatc aattgaatga cattgataat agcgccgacg atggcgttaa
      781 tcagagtaaa catccagtac tctttacgac gtgcgcgacc agagaaacca acataatttt
      841 ttaatacgga taaataccac tgcataaaaa catcccttta attaaaagtg acgaaagtct
      901 attccgggta ttgtgtaaat gctattcgta tctttctttt tcggatgtta taaaggtttt
      961 gatatttaag ataaatacat ggttattttt ataggtaatt tatataacga cattgaaata
     1021 ttttaaataa acattagtaa attattcatt catcaacatg atgataaaag ccggatgagt
     1081 cagacatccg gcgagcagat ttgttttaag gttccagttt tggatcaggt ccaaagcggt
     1141 tttcgcctgg cgtacccgcc tggcagttaa agacgatgat aataagccag ccgataaacg
     1201 ggattaagaa tagcagtgcc caccacgccg agcggtcggt gtcgtgcagg cggcggaact
     1261 gaactgccca ccacggtaaa aacaccagaa taccgtagat tgtcgtcagg atgccttcgc
     1321 caccagcgcg ttgccagcct aacattttat ccagcagccc cagcacgaac gtaaagatga
     1381 tgttgaccag aataaacatc cagtactctt tgcgccgcgc acgaccccgg aaaccgacat
     1441 aattttttag tactttcaga taccagtcca tttttgcctc aattttgctc aatcacttgt
     1501 ttttaaagca gcaaattaag aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg
     1561 aattaataaa gtgctgtgaa ggcgcgctaa gactgatgac aagcgcaaaa ttgccagatg
     1621 cgctacgctt atctggcccg cgcggtcatt gcaacatatt gaatataaaa gttttgtagg
     1681 tttgataagg cgttcatgcc gcatccgaca tgaacaacat gcacttagct aattatcaga
     1741 ggcacctcgt aaggtgcttt ttttaaccta agcgagcatc tcgtccatgc ggttcatcga
     1801 gatcctgcca cggaccaatt gaaataatcc gcgtagggtt gatggtctta tgactgcgga
     1861 agtaatgatt acggatatga tcgaaattga ctgtttcggc aattcccggc atctggtaga
     1921 tatcacgcag gaagccatac agattaaggt aatcgctgat gcggtgctta tcacacttga
     1981 agtgggtcac atacactgga tcaaaacgga ccagcgtggt ccacaggcga atatcggctt
     2041 cggttagctg gttgccggtc aggtaacgat gctgacctaa aatctgttcc agtcgcgcca
     2101 gcgattcaaa cactttctcc accgcttcgt cgtaggcttg ctggctggtg gcaaaaccag
     2161 ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg tcaattttcg
     2221 tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca aacgcggtat
     2281 taaacatgcg gatgatttct gctgattcgt tgctgacgat ggtgtggttc tttttgtccc
     2341 acagcacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga taaagctgat
     2401 acaaaaattc atgttgatag agcgtgtctc cggttgctcc cggaaaactg tcatcaaagg
     2461 tccagccgtt ttccagcatc agcgggttca ctacggaaac ggaaataaac ggttccagtc
     2521 ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt gaaacataga
     2581 gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcca ggagcgccat
     2641 cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg ccggtagatt
     2701 tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccatt gttctctcct
     2761 ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata gcgcgtgctt
     2821 accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta attgccagca
     2881 gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg acgccttcag
     2941 caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta aacagggctg
     3001 tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca agcagaatca
     3061 ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg gtacccgcgt
     3121 aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa atgcgcgcta
     3181 ccagtacacc aacatcttct aattttttca tcatactctc cactgcgcca ctcaggctgc
     3241 tgattgtttt tttgtgcaag ggcgcggtat tagcgtcgtt gctgtcgatg gagagaatca
     3301 taaacgtggt gaatgatgat tgttagcaag gaaaactgtc aaaaatcttc aaaaaatttg
     3361 atgggtaagg ccggaatggc tccggccaga gggaagttaa ccgcgaagct gttgctgctt
     3421 gagggtcgtt ttaaccagac gccaggcgct ccatacgcca aaaccgcgtc tggcccagcg
     3481 gaccagcata ttaggatggc gaatcgtcca gatcgccatc acgctactgc caaccagcgc
     3541 ccaggagcgc agacttagca gcatattcca gcgacgatcg taagcgcctg ttgcctccag
     3601 ccattcacga cgactggcag aaagatccag ccgttgttgc tggatctggc taagcagttg
     3661 cgccttacgt cgttcacgtt cgactttact gctcacggga ctcctcctcg agcagctgcc
     3721 gatcgtttgc cagctcatgg cgtgtatggc gcagcaaggt ggatttacgc gatttacgta
     3781 acgtccagat accgccaatc agtgccagta gcagcaacac cacggtggtg gcaatcatcg
     3841 cattcaggcg atattgcgga tcaaccgccc aaataattag caccatcagg ctcataagac
     3901 caaatgcagc gaaaagcatc gtcaggccca gcatcagtaa aagttgaaag agattagctt
     3961 tttcctcttc cagctcaacc accgccagcc gcagacgtgt ttccaccatt tcaaccatga
     4021 tggaaacaat tcgctgcccg atgcccagaa cgcttttacc gggcccttgc gcgtgatgag
     4081 tgtccgccat aattaacgac gcgacagcag aacgccgagc actacaccga ttgcagcgcc
     4141 aatgcccacg cccgtccatg gattttcacg cacatactca tcggcacgcg ccgccgcgac
     4201 acgggtttgt ttggcgatgg catcaccggt ttcacccagg cgataacggc tctgtttcag
     4261 tgcctgctcc gctttgctac gaatcttact caactcttct ttcgacttct cgccagatga
     4321 gctaagcacc tcttccagcg tatcggaaag ggatttcaac tcagcacgca gatgttccgt
     4381 agtgtgttct ttcgacatag ttttctccag gtgagtaagt agtgactatt gtgagttagt
     4441 agtcgcgcgc ttccagcttt ttcagctctt cctgcgcttc tgccagtttc cgttcgcgtt
     4501 tggcaatctt atcggcatcg cctttttgct tcgcctcggc taaatcttgc tggcgttccg
     4561 ccacctcatc tttctgcttt gcgattttct tctgatgatc ggcacgcagc tggctatctg
     4621 aacagttggc ccggacttca ctcagggctt tattcagacc gtcaatacga ttctggtttt
     4681 ggtgtttttc ggcatagctg atctccttaa ggatattttg ctccttttcc tgacacaggg
     4741 tagtggcata actaccggca ctaagagcaa aaagagaaac agctaaagcg atgcggtatt
     4801 tcattcttga accttccatt gtgattcgga cctattacaa aggaacgatt gtccagacaa
     4861 cgaagcggta gtgttaacca ccgcctcatt aatcatagac agttacggtg aaatcaccaa
     4921 agtgagtgat ctgtttcgga caacaggaag ttatccgaag cgatgagagt tatcccgtag
     4981 ccgggtcagc cactgcatag cctgactgtt attgtcctgc tgcgcaatga tgtagccttg
     5041 gggcaatgtt ctgtcgagga ccgcttttgc agcggcgctc tggtcgctgg agtcaaaggt
     5101 aattaacaga gtgtcgtttt tgggggtgat acttttgaaa ggaatgccat gagcgtcaag
     5161 gtgatgccag attgaaaaac cgtctggcat cgttgtgcct tgatgaacgg cacgaatcgc
     5221 cagcgtagac tcttgctggc gaaccgcgga ccaggccagc aacagcgtgc cgaccagaag
     5281 taaaacagcg ccggaccagg ctagctggcg aagcgacatg cgaggtattt gcatccctta
     5341 cccccgattt ccatatttct ttttccataa tacgaccaga gaacctgcca ggccaaacac
     5401 cagcagcacc accgggagca gcatcaggca tgacatcagc tggtcctcgt actttaaaaa
     5461 taccggcgtt ttgccgagca tgtaacccag agttgtcagg atcaataccc acagcagacc
     5521 gctcatccag ttgaaaaact gaaagcgcgc gttattcagc cctgataacc cggcaatcgt
     5581 cggcagcagt gttctgacaa acgcaataaa gcgaccaatt aacagcgccg ataaaccgtg
     5641 tttatgaaaa agatggtgtg cgcgttgatg ataatgcgcg ggtaaatgag atagccagtt
     5701 ttgtacggtg cgggtattgc ccagccatcg cccctgaata tagctgaccc agcagccgag
     5761 gctggcggca acggtcagca gcagaatcgt ttgcggatag cccatcgcgc ctttcgcaat
     5821 caacacgccg accaatacca gtaaactgtc gcccggtaaa aaggccgccg gaagcaagcc
     5881 gttttcaagg aacaaaatta caaacaagac aaaatacaac atgccaatca tcgatggatt
     5941 ggccagggtt tcaaaatcct gcgcccacag ggcttgcagc aattgggtca aaagttccat
     6001 tcattattcc tggtgaatcg gttaacgcca cgctggtttt aaaagatgca ctaccgcaat
     6061 gttattgctg ttttggtatg ttctgcgggt ttgatacatc aacacagcct gaactattcc
     6121 ctgttaaatc gtgagataag aaagcattca aaaacataaa ttcaaattga atctaattgt
     6181 aacaaaggtc aacgttgttc gttatcaaaa ttacatgtcc gtggagtctg attttgctga
     6241 atgcagggga gggtggcgag aggatttaca caggctgaca ctttatactt ttcgcttacc
     6301 gacgagacaa acaggcgcgt cgcagcgcct gtcggacagg tatttcattt actgccactg
     6361 gtggccgtat cgagatgcac taccgggttt tcggcgaaca gatagcggtc ggcattgaac
     6421 tcgaagtcgt cgctggtttc gttgaataac atgatcttgg tgttttccag gtgctgccac
     6481 attgccagct tagcagcatg aggatctttg cgaatcagcg ccttgaggat ttgatcgtgg
     6541 tcatcacacc agttatcgac ggtacgggaa tcaatgtgtt cgtgcagttt tttccagtac
     6601 gggttatggc tacgctgggt ccacattttt tcaacgatag ccgccagggc ggagttctgc
     6661 gtcgccaggg ctacctgaat atggaactgc aaatcccact cggaatcacg gaagcattgt
     6721 tcgccgcgcg cctgctcctg gatggccatc aatttcatga tgtcctgttt cgttacctga
     6781 gttgctgcga actcggcaat attactttcg atgagttggc gagcctggag taactcaaac
     6841 ggaccgtaat tggcgaattc catattattg tccgcagcct gctgatggcg cggctggttg
     6901 gaaaccacgt gaatacctga acctttacgc acttcgacat agccttcaac ttccagcatg
     6961 atgatggctt cacgaactac cgtacggctg acgttctttt catcggcaat aaagcgctca
     7021 gcaggcagtt tatcacccac cagatagacg ccctgttcga tgcgctcttt caggtcagcg
     7081 gcaagttgtt gatacaaacg gcgtggttca gtgatttcca tatgcgctcc aggcatgata
     7141 cggcagacta tatttgttat accacttttg cgagtttagc tctactcgca gtgatgaaaa
     7201 agccgcctga gaaggcggct ttgaagcgaa actaaaactt acacgaagcg ttaatgttgc
     7261 ggtgcgggat cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg
     7321 gtccagataa ccagcgcacc cagcaggtca aatactgcca gaactgcgaa cagcgggctg
     7381 aagccgatgg tgtcagccag tgcaccgaca accagcgcaa acagcgtact tgccagccat
     7441 gcggacatcc cggttaaacc gttcgccgtt gccacttcgt tacgaccaaa cacgtcggaa
     7501 gagagcgtaa tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat acacagcagc
     7561 ataattgcga catacgggtt ggtgaacagg ccgatcatac ccgggccaat catcagcact
     7621 gcacccagcg ttacgaccat cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg
     7681 aacagcggcg gcaggtaacc accgaggata caaccgaggt cagcaaacag catcggcatc
     7741 caggcgaaca tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat gaacagcggg
     7801 atccacgcgt taaaagtacc ccaggccggt tctgccagga aacgcggcag cgcgataccc
     7861 cagaactgac ggttacgcag gatctgacca acggacattt tcttcgccgt actcacctgg
     7921 tgctgggctt cctgaccatt aataatatag tcgcgttctt catcggtcag atgcttctgg
     7981 tcgcgcggat gtttatagaa aatcagccat gccatcgccc agataaagct caatgcaccg
     8041 gagatgataa atgccatctg ccagctgtgc atcacgattg cccataccac cagcggcggc
     8101 gcaatcatcg caccaatcga agaacctacg ttaaaatagc ctactgcgat ggaacgctct
     8161 ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc tgcttccgcg
     8221 gcaccgaccg caccacgagc aacagccagg ccaccccagc tacctgccag cgcggttgca
     8281 ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt acccagcaca
     8341 tccagtacat aacctgctac cggttgcatg accgtataag cagcagaata ggctgcgatg
     8401 atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg cgcagctgcc
     8461 gccacagtgt tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag tgcgatcata
     8521 taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt atatccgcag
     8581 gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa
     8641 aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc
     8701 ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg
     8761 atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag ccatcacaaa
     8821 tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg
     8881 cagagagatt taccactttg agagtaattt ttttaactac gtttattgat ctaactcacg
     8941 aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca
     9001 agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct
     9061 gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt
     9121 cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct
     9181 gtatgacatc tggctgaaag gcgatcacta caaatggcgc gctatgcgta ccaacggtgt
     9241 ggccgagcgt ctgtgtaccg gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc
     9301 gactgttccg cacactatcg gcaacccgtt ataccactgg acgcacctcg aactgcgtcg
     9361 tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga
     9421 atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa
     9481 cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat
     9541 cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt
     9601 caacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac
     9661 cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc
     9721 cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa
     9781 cgaagcggaa ttggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca
     9841 cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg
     9901 cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa
     9961 actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg cagaagagtt
    10021 gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctgtactg
    10081 tctgaacccg cgcgataacg aagtgctggg caccatgagc ggtaacttcc agggcgaagg
    10141 tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat
    10201 ggaacgtcag atgacccaac tggcgcagct cggtttgctg agccgctttg tcggtatgct
    10261 gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg
    10321 ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acctgctggg
    10381 cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa
    10441 ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc
    10501 gctttagcgg atttggctga aggcacagaa gtcagtgtcg ataaccaaac tgttaggctg
    10561 cgccaggatg ttgctcgtgg acataaattt gcgttaacga atatcgcaaa aggggccaac
    10621 gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac
    10681 gttcacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaacct
    10741 gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct
    10801 aacggcgatg tcggggtgcg taacgagctg tggatcctgc caaccgtggg ctgtgtcaac
    10861 ggcatcgcgc ggcagatcca gaaccgtttt ctgaaagaga ccaacaacgc tgaaggtacc
    10921 gacggcgtgt tcctcttcag ccacacctac ggctgctcac agctgggcga cgatcacatt
    10981 aatacccgca ccatgctgca aaacatggtg cgccacccga acgcaggcgc agtgctggtg
    11041 attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgct gggcgatatc
    11101 gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccgga
    11161 atcgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa
    11221 ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact
    11281 gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta
    11341 ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac
    11401 gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact tcaaacagta ctttattgcc
    11461 catgaccagc cgatctatga aaacccatcg ccggggaaca aagcgggcgg tatcaccacg
    11521 ctggaagata aatcccttgg ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg
    11581 ctgcgttacg gcgagcgtct gaaaacgccg gggctgaact tgttaagtgc gccgggtaac
    11641 gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact
    11701 ggtcgtggca cgccatacgg cggctttgta ccgacggtga aaatcgctac caacagtgaa
    11761 ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa
    11821 gcgatgccgc agttgctgga agaatttatc gataccatcg ttgagtttgc caacggtaaa
    11881 caaacctgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg
    11941 ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt
    12001 gcttttccat acctgataag gatttatcat gaccgcgtat tggctggccc agggcgtggg
    12061 tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa
    12121 aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta
    12181 tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct tacgcacgcg
    12241 cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt
    12301 ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt
    12361 ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat
    12421 tcacaacttc tgggcggggt cgataggcgg cacgatgatt gagggtagtt ttctgcttat
    12481 gaatggcctg aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa
    12541 agtagagaaa acccctcccg ccatagacga aaggggttaa acaattaatt acgcagggcg
    12601 ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt
    12661 gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgataataaa gccgacggca
    12721 accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag tgggatcagc
    12781 agcaaagaac cccccgccac gccggatgcg ccacaggcac acagagaagc cactacgctc
    12841 aacagcagcg ctgtgggcag atcgaccgga atacccagcg tattaaccgc agccagcgtc
    12901 aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggctcccag cggaatagaa
    12961 acggaatagg tatcgcgatc cagattcagc ttttcacaca gtgccatatt caccggaatg
    13021 ttcgccgcag agctgcgggt gaagaaggca tacacgccgc tttcgcgcag gcacagcagc
    13081 accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc
    13141 gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg
    13201 gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccaat cggtgcgaag
    13261 cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac caggtttttg
    13321 gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg
    13381 atgtagttac ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat taccagcccg
    13441 cgcatcactt ctacaatgcc tgacggtggc gaaatatcac ccgcgctact ggataagtgc
    13501 agggtagaag ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga gaaggtaccc
    13561 agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg gtggttagca
    13621 atagatgcca tcaccagcat caacaccagg atgggggcaa cggctttcag tgcgccgacg
    13681 aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgagatcca tgccagaaga
    13741 atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg
    13801 aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat
    13861 ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt
    13921 ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aggtcatatt
    13981 ttgcaactta atggttacat ttatttgaca taaaaataaa gccaacgtgc ggcttccaga
    14041 ctgaggacta cgtcaaaaaa ggactcggtc agtcccctcg ccccgtttgg ggagagggtt
    14101 agggtgaggg gaacaggcca gcatcggtgc gaaaaattac cctcaccccg accctctccc
    14161 tggaagggag agggagaaaa cggtgctcat gttgatgaca gggtacacgg cttaggcaga
    14221 ttaaaaatca tccaacccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    14281 acgtcatcac caggatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    14341 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    14401 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    14461 tcgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    14521 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    14581 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    14641 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    14701 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    14761 acaggatcca gtcgaactgc gaaatcagcc agctgccagt gaagatcatg atggtacgca
    14821 gaacaatcgc cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc gccggaacgg
    14881 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gacttctcaa
    14941 tcagataacc cgtgaggaag gccagcgcct gtggatcggc aacggcgcga ccttcggttt
    15001 gtaccagata ccaccagaaa gcagcgttaa acagtaacga cagcgtcacc cagaccagcg
    15061 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgtccc tgcaacaaca
    15121 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    15181 cgacagtatt catagaagtt ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca
    15241 gccgctgctt tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag
    15301 gaatacgtct gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc
    15361 gtaatgacga tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga
    15421 ccattcagtc agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat
    15481 agttttacgc tatctgccgg aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt
    15541 ttggctgtga tgcgactgac cgccagccct ggatgatcca ccagatgctc atccaccagg
    15601 gtagcgaaca gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc
    15661 tgcatctggc tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc
    15721 accagcgatc ccgcttcgcc ctgaatctcg cttgccagga cagaatcgct gacctttgag
    15781 tgctgcaagg tgacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcatctaca
    15841 ccacttgcca gtaaactggc ggttgcctgc acgcttttcg gctcaccaaa taacgctacc
    15901 gccgacgcca ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgccggatta
    15961 aaggtgttgg gattctcacc gtccaggtaa cgttgatacc gcgaggaata ctggcaatag
    16021 ttgaaaaaaa ctttccgcag tttgccgact ttcggcagcg cctggcgtaa cagatgaaag
    16081 ttaggcaggc tggccgtttt aaatgcctca aacagcacca cctgattttc acgcgcgcag
    16141 gcaatggctg catccacttc cgccagattc gacgccagtg gtttctcaca aatcacatga
    16201 attttgtggc tcaggaaaag ttgcgtctgg gaaaaatgca gggcgttcgg gctggcaata
    16261 tacaccgcgt cgatagcgtc actttccgcc attgcttcca gcgaagtaaa cagatgctcg
    16321 acagaaaact cattggcgaa gtgctgggcc tgttcaaggc tgcgggaata aacggcggtt
    16381 aacttatatt taccgctttc atgtgcggcc tccacgaact ggtgagttat ccagttcgta
    16441 ccaatcacgg cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa
    16501 tgtagcacgc ctttttcatc ttcgacgtgc gcggatacgc attatttctg cgccggacaa
    16561 ccatgctcac taaacggacc aataatgacc gacggcccga gatattttgg ctgacgattg
    16621 aagattttcg cgtcaatcac cgcgctgact ctggcgagtt tctctcgcag acggacaatc
    16681 tttccgcgtc ctttttccag gtctggtgcg ttaaacccga tagcatcgat gccgtattgt
    16741 tttgccagcc agatggcgcg ttggttatga aattcctgcg agataatggt gatatggttc
    16801 tccccaaaca ctttattggc acgcaccact gaatcaagcg ttgagaatcc ggcatagtca
    16861 cagaagatca cttttgccgg tacgcccttt gcgatcaaag cctgctgcat gcctgacgct
    16921 tcatcgtaat ttttacgtcc gttatcgcca ctgaccagca gccatttcac ttttccggcg
    16981 tggtacaact ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctggcc
    17041 cccagcaata agcccacgtt acgcgccgga acgacgttga cgtcgctcca ggtcagctgt
    17101 ttgctggcgt tgaccattac gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac
    17161 agtaagcagg caatctttag agtccggcga gaaaaacaaa tacggagaag aaggcgggca
    17221 aatgcgcgca gcatggtcgt tatccttaac gaaagggggg aggtcgattt taggcgctaa
    17281 agaaagaaaa gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg
    17341 ggttagccac agtcgggtgt cgaactcaag ctggtggtac tggggttcca tatgacagca
    17401 caactggtaa aacgctttgt tatgctcttt ctccttcagg tgcgccagct cgtgcaccac
    17461 gatcatgcgc aaaaacggtt ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc
    17521 ttttgctttc agcttgccgc cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt
    17581 ttttagcaca tggatcttat tgtcatacat cactttattg atcggcgggg cattacgcag
    17641 aaactgattt ttcagatcct gagtatattg ccagagggct ttatcggtgg cgtagtcgtg
    17701 tgttcccgga tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg
    17761 cacctgggaa agtagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc
    17821 ccacgaaaaa gagaaaaagg gtatactcac gcaccctttt caggggcaat gtcgaaattt
    17881 tatcattcag gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa
    17941 cgttttccgg cgacggatga cgttaacccg ctacaggcgt gggaagcggc ggatgaatat
    18001 ttgctgcaac agttggacga cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc
    18061 tttggtgcgt taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac
    18121 atcagtgaac tggcgacgcg cgagaattta cgcctcaacg ggattgatga gtcgagcgtg
    18181 aagtttctcg acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg
    18241 ccgaaaacac tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgcca
    18301 caaacacgta ttattgccgg tgccaaagcc cgtgacatcc acacttccac gctggaacta
    18361 ttcgaaaaag tgctcggccc gaccaccacc acgctggcat ggaagaaagc gcgcctgatt
    18421 aactgcactt tcaatgaacc accgctggcc gatgcgccgc agaccgttag ctggaagctg
    18481 gaaggtactg actggactat ccacaaccat gcgaatgtct tctcccgcac cgggctggat
    18541 atcggcgcgc gcttctttat gcaacatctg ccagagaatc tcgaagggga gattgtcgat
    18601 ctcggttgcg gtaatggcgt tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa
    18661 gtggtgtttg tcgatgaatc gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc
    18721 aacatgccag aggcgttgga tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg
    18781 gagcctttcc gctttaatgc tgtgctctgc aacccgccgt ttcatcagca acatgcgctg
    18841 accgataacg tcgcctggga gatgttccac cacgcccgcc gctgcctgaa aatcaacggc
    18901 gagctgtata tcgttgccaa ccgtcacctg gattacttcc ataaactgaa gaaaattttc
    18961 ggcaactgca ccaccatcgc cacgaataat aaatttgtgg tgctgaaagc agtgaagctg
    19021 gggcgtcgtc ggtaagattg acagctacct gccagataag gcgtaaacgc cttatctggc
    19081 atacgtttag cacattaccc ccaggtcgga taagatgcgg caagcgtcgc atccggcaat
    19141 gtctgcccag ttctggaatc gcgctccata aaacaaaaaa tccctgtcac catcctctac
    19201 attctcttgt ttagcgttgt ttaacgttta ttcttccgtc acacagataa attcatccgt
    19261 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    19321 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    19381 ggagttggtg gctctaggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    19441 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    19501 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    19561 catacgtgaa gttatgacgc ataaagaata cgattttttt accgctgttc atcgtactaa
    19621 ggggaaaaaa tgattgcgat tgccgacatc ttgcatgcag gagaaaagct aactgctgtg
    19681 gcaccttttc tggcgggtat tcagaacgag gaacaataca tccaggcgct ggaactggta
    19741 gatcatctgc tgctcaacga tcctgaaaac cccttactgg atctggtgtg tgccaaaata
    19801 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    19861 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    19921 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    19981 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    20041 gattaacgcg
//
LOCUS       DAZFKY010000081        19282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFKY010000081 DAZFKY010000000
VERSION     DAZFKY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            50..568
                     /locus_tag="ACHKCL_002570"
     CDS             50..568
                     /locus_tag="ACHKCL_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5763522.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            584..1123
                     /locus_tag="ACHKCL_002571"
     CDS             584..1123
                     /locus_tag="ACHKCL_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5763523.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHKCL_002572"
     CDS             complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHKCL_002572"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5763524.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHKCL_002573"
     CDS             complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHKCL_002573"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5763525.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4490..5860
                     /gene="xseA"
                     /locus_tag="ACHKCL_002574"
     CDS             4490..5860
                     /gene="xseA"
                     /locus_tag="ACHKCL_002574"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5763526.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5857..6072)
                     /locus_tag="ACHKCL_002575"
     CDS             complement(5857..6072)
                     /locus_tag="ACHKCL_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5763527.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHKCL_002576"
     CDS             complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHKCL_002576"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5763528.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHKCL_002577"
     CDS             complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHKCL_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5763529.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHKCL_002578"
     CDS             complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHKCL_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5763530.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHKCL_002579"
     CDS             complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHKCL_002579"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5763531.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHKCL_002580"
                     /gene_synonym="gcpE"
     CDS             complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHKCL_002580"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5763532.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHKCL_002581"
     CDS             complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHKCL_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5763533.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13386..14540)
                     /locus_tag="ACHKCL_002582"
     CDS             complement(13386..14540)
                     /locus_tag="ACHKCL_002582"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5763534.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHKCL_002583"
     CDS             complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHKCL_002583"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5763535.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15262..16116)
                     /locus_tag="ACHKCL_002584"
     CDS             complement(15262..16116)
                     /locus_tag="ACHKCL_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5763536.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16116..16937)
                     /locus_tag="ACHKCL_002585"
     CDS             complement(16116..16937)
                     /locus_tag="ACHKCL_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO5763537.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16930..17559)
                     /locus_tag="ACHKCL_002586"
     CDS             complement(16930..17559)
                     /locus_tag="ACHKCL_002586"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO5763538.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17556..>19282)
                     /locus_tag="ACHKCL_002587"
     CDS             complement(17556..>19282)
                     /locus_tag="ACHKCL_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO5763539.1"
                     /translation="TLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRY
                     SDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVP
                     ANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRH
                     ICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNW
                     MQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIE
                     FIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDY
                     DWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKS
                     APHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLT
                     AKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDR
                     GVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQT
                     MLVEVEKA"
BASE COUNT         4762 a         5234 c         4694 g         4592 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcgggtctg gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg
      361 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacgtat aaggaaggca ccaaagtgta tacctctact caggtgggta aagagtgcca
      481 gtttacgaca ggtttagccg ttgttattac cacgacgtat aacgaaacgc gtattcagcc
      541 aaataccaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      601 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      661 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      721 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      781 ggctgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
      841 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      901 cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg
      961 agaaaacgaa tttatcgatc aggagctgaa gcacaaagcc gcgcaaactg atgtcgtgca
     1021 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1081 gggcaaagca gaagagaata aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1141 aagcctataa ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1201 tagtgcaggg tcaaatcatt cccactcaat ggtagctggt ggcttgccgc tgatgtcata
     1261 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
     1321 atcgtacggc agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
     1381 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtactggcag
     1441 gaagacggtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat
     1501 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagaac
     1561 acgaacgcca aggcctggtc ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc
     1621 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1681 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1741 tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1801 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc
     1861 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1921 ttctgccggt acgtgaacaa tgttaagacc aaagtgatcg ccaaacatat ccagaacctg
     1981 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc
     2041 gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag
     2101 gatgacttta tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt
     2161 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg
     2221 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     2281 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     2341 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     2401 tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg
     2461 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2521 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2581 cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct
     2641 tggattgaag tcacgaattt gtgcttctgt cacatcccat gcccacagtt cgcagtaaac
     2701 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2761 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2821 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2881 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2941 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaaa
     3001 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     3061 taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg
     3121 aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat
     3181 ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc
     3241 atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     3301 aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg
     3361 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     3421 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca
     3481 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3541 tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaacg
     3601 tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact
     3661 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct
     3721 ttctggaaat ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3781 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3841 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3901 gtaacaaaac gcacgtcacg accggtgata atccccacca gttcgttttc ttcggtaacg
     3961 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     4021 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     4081 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     4141 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     4201 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     4261 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     4321 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     4381 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg
     4441 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4501 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4561 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4621 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4681 caacagcaac cgccgggtga ccttccgccc acagcatggg caacaagttt tagttcgcgc
     4741 caatattacg ctctacgagc cgcgcggcga ctaccagata atcgttgaga gtatgcagcc
     4801 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4861 aggattgttc gatctgcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4921 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4981 tccttctctg ccggtgatca tctaccccac cgccgttcag ggcgatgacg cgccggggca
     5041 aattgttcgc gccattgaac tggcgaatca gtgcaatgag tgcgacgtgt tgatcgttgg
     5101 gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     5161 gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     5221 tgccgatttt gttgccgatc tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag
     5281 ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc
     5341 gatggattat tatctcgcca accgcacacg tcgctttacg caaattcatc accgattaca
     5401 gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa
     5461 acggatgagc tttgcgctgg aaaatcaact taagcgtgcc gggcaacagc agcagcgatt
     5521 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat
     5581 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga
     5641 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5701 tggatacagc gttactactg ctactgacgg caaggtactg aaaaaagtga agcaggttaa
     5761 agcgggtgaa atgctaacca cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa
     5821 catccagccg gtaaaaaaat cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg
     5881 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctaccgcac cgcaggcttt
     5941 taacacctgc aacggttgat ggcagtccgg gcaaagagct ttcatttcaa taactttccc
     6001 gcaggatggg caatacgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca
     6061 atgcagttcc atatgaattc ctccggtagc cacccctcta cattcataga gggaatggca
     6121 gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt
     6181 ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct
     6241 ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc
     6301 aagtaacgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc
     6361 ggcggattat aaccaccggc gtgtgcatat ttcagcttca cacgacgacc gcgtaccagc
     6421 ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca
     6481 cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg
     6541 ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc
     6601 agcgtttctt tcacctgctc tttcacttcc tgactcaggc catcccactt attcaccaca
     6661 atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa
     6721 ataccttcgc gcgcatcaat cactaacatc accacgttgg cgtcttcaat ggcctgcaac
     6781 gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca
     6841 gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta gatactgtca
     6901 cgcgtcgtac caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg
     6961 agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc
     7021 gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag
     7081 tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagtagc
     7141 acatgctcca gcagacttaa tacgccacga ccgtgagacg cggcgatcgg gtagatttca
     7201 cctaaaccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg
     7261 tttgccacca ggaaggtcgg tttttcacgg gagcgcagat gtttggcaat cgcttcatct
     7321 gccggcatca ggcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc
     7381 gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg
     7441 ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca
     7501 cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat
     7561 aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa
     7621 gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag
     7681 gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta
     7741 atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt
     7801 tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgacg
     7861 ttaatccagt gcaaataacc ttcactgtca ccgaccacca ggttgccatt atacagcacc
     7921 ggagaagtca gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac gccgccatca
     7981 atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg
     8041 atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca
     8101 agcgccgtca ggttaccatt ataggccagc gcgaaaacaa cgccgttaac aacggcggga
     8161 gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcttg ggaaatacgc
     8221 tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgccc
     8281 cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa
     8341 ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac
     8401 tgaccgttac tggtgtggat taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct
     8461 tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc
     8521 tgcgcctttt cgctgccaat gtagacatgt ccaccagaca cggtcacacc gccagaaagt
     8581 aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct
     8641 ttgccatcat ccgcattcag cgcttttact aaaccagcgc ggtccgctgc atagacaacg
     8701 ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa
     8761 gtgctccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt
     8821 accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt
     8881 cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat
     8941 tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca
     9001 tgcactacgc gcaccttgct tatcaccttt gctcagcaat gcttcaccac gcaggtcggc
     9061 aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc
     9121 ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc
     9181 atcgctcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt
     9241 gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc
     9301 agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac
     9361 cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta
     9421 gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt
     9481 ttcagcaaaa aagcgtttaa tcgcttctac ctggtcgttt tcgttctcgt aaatttccac
     9541 gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc
     9601 gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagccact
     9661 tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt
     9721 ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa ttcatcacgc
     9781 agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat
     9841 atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga
     9901 cgttcgaggc ccatcgcaaa accgacagcc ggtgttgcac ggccgcccag ttgttccaca
     9961 agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgagact gttagtcacc
    10021 cactcgaaaa cggtacggtt gtagtaatcc agaccacgca ccagacgctg gtttacggtg
    10081 taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc
    10141 tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt
    10201 tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc
    10261 ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc
    10321 agcgaaccga tagaattcag ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac
    10381 cagcgggcgg tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact
    10441 tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac
    10501 atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg ctcgatgccg
    10561 gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc
    10621 tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat
    10681 agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg
    10741 tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc
    10801 atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg
    10861 aaccctcaac gcttccctca atgtttcggg agccatgcgg gttcaatcat acacgggaag
    10921 cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt
    10981 cgtccagctg actggctttc gcacgaatgc gtgcctccag ctggtcgatc atatcgttgt
    11041 tgtccagacg gtctttgcgc acgccatctt catagagacc gcttttcttg ttgccgccgg
    11101 tgacgccgag tgtagaaacc agcgcctcac ccgggccatt caccacgcag ccgataatcg
    11161 aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta actgtaccga
    11221 taacatcaaa ttcctgacgc gaacaggtcg gacaggcgat gaagttgata cctcgcgaac
    11281 ggatacgaag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggccg
    11341 ccagtgatac gcgcagcgtg tcgccgatgc cttcagacag cagcagacct aaaccaatgg
    11401 cggattttac tgccccgctg cgcgcgccac ccgcttcggt gatccccaga tgcagcggct
    11461 gatcgatttg ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt
    11521 tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca tggcgcatgg
    11581 cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca
    11641 gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca
    11701 ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc
    11761 cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc accagcggca
    11821 cgttaacctg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga
    11881 cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga
    11941 catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg
    12001 gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca
    12061 tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt
    12121 cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata
    12181 ctggatctgt actgcggctg gcgcaccaat tttcagtttg tacggtgcct ggccggttaa
    12241 gttcaaatta ccgtctttac gctgcatacc gctaaacaat tttttaccgg tagcatcagt
    12301 gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccaccgg
    12361 cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc
    12421 cgttggtgcc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt
    12481 ctgttgcgga tcaacagctg gtgctggcgc agttacgaca ggtgtttgcg tgttggttgc
    12541 ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt
    12601 atttaacgga acactctgcc cctgctcgct attactgctc agttccgccg aagattgatc
    12661 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
    12721 caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt
    12781 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg
    12841 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
    12901 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
    12961 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag
    13021 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
    13081 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
    13141 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
    13201 taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca
    13261 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
    13321 aacttaatac ttcattcata aaacattacg ccacggtaca taaagtaacc gtggcgtaat
    13381 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
    13441 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat atcatcacca
    13501 cgcgttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
    13561 atacggctgt tcgagctgcg tccatacggc gcagccggga acgggttcca cgggatcagg
    13621 ttgatcttac acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg
    13681 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
    13741 ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc
    13801 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaaca
    13861 tcgatcatat cgcccagttt atccagcgct ggaactacac cggaagtgga aagcgtcacg
    13921 cgacgtttag acagaccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
    13981 ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattgggcgc
    14041 tgaccggtga cttttgctgc gccgacgatt ttcgccgcac gccacacctg gccaataatt
    14101 tccgataccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc
    14161 agcgcacacc ccacctgcga agagacgcag agcgtagcgc ggtcgtcttc cgggatgtac
    14221 accgtttcga cacgctggtc acccacggca atcgcccatt taatggtgcc gtcagatgaa
    14281 cgctgttctt caaccacttc cggtgcgcgg atctccgcca cctctttcag tttgccgcgc
    14341 aacactttgt tgatgtcggt catctcatca aaattgtcgc agcaatagtg atacatccac
    14401 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
    14461 tgctgacgat tgagatccag caggttgatt tttccatctt tggtggtgac gttttcaggt
    14521 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
    14581 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
    14641 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
    14701 gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg
    14761 aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac
    14821 cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt
    14881 tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat
    14941 caaagaacgg ttttccatcg tgttcagcat aaaagccacg tgcctgttca acggtcaggt
    15001 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatattac
    15061 caatgacgtt ttttgctacc gcgttcggtt tgataatgga aaaagtacgt tcaatagcca
    15121 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
    15181 ataatgagca acagagccgt tgactattga tgaaggtaac atttttttaa aataaaatga
    15241 gttttagcaa caactcgact tttatggtgt tctcatccca taacgatgac gttggcaata
    15301 aagacaattt tgcgcatttt gctgaaaagt aagaaaggat ttttgacagg ttttacagac
    15361 tttgcgatta gcatgaagat tcacaatttt tgccggtaga atcgtcaatt caacggtcat
    15421 tgcctgatga aaacaaacag caatacaatt ttggcatccg ttacacaagg catcatttat
    15481 ttgtaaatgg ccttcgttaa gcgaaaagac ctgttgctcg caggcccgcc agcatgcacc
    15541 gcaaagctga cacttttgag gatccatcat cgggcgagca tcactaatct ggggccataa
    15601 ataatgtaac cgacgtaatc ctggttgtat ttgtgtcatc tgaacatccg cacgctgcac
    15661 atggaacaaa cgacgccggg aaacattcac ctcactggta tgtggagata ccacagacca
    15721 gccttcctcg ccatattgcc gtaagcattc gtttaacagc aataatgcgc gctcccatgc
    15781 cttcgcctga ctcagatcaa tcgcgatcgc tgtgatcccc tgctctttat gccatagcaa
    15841 aagttcttcc agcgtcggcg caatagtggt gaaaggtgcg accagggtgt gattgcaaaa
    15901 ataacgtaaa ggggaattaa cgccacgaat agcctcttgc ggacaaataa actggcaaac
    15961 cgcacaatca atacattgtt ccgcgtcgac cgctatgccc tcttctgtca gcgttagcgc
    16021 ctgtgccggg caagcctcga cacaggcatg acatgttgca aaacgaaagc gttggcgcgc
    16081 gcattccgcg ccaatcgtta tgttatgact taacattagc cgatactaaa aaagacaaaa
    16141 cgcagcataa cttcactccc cactagcatt acgctaccga ataacgccgc cgggatattc
    16201 tttttacaac caccgacagc aaagaacatc atcccaatga cggaaattcc ccagcttagt
    16261 gtcagtatgg gttttaactg tcccagcatc gctaatggcg catgtggcaa tgtggctatc
    16321 tgcatcgcat ttgccgttaa atcccctatc cacaacggct gaaccaacaa acgcagcaga
    16381 accaggagag cgatcaccaa cacgcatcca ttacgcaggg catcaatctg cggcaaaatt
    16441 ccgctaatac gaaacactgc taccagcgct gaaccgagaa ttcccactgt acctaagaac
    16501 ataatcagcg tgttgtaatg ctgccaggtg ataatcgacg tatggatgta aacttgcgcc
    16561 atgcagaaaa catcaacaat cccgaccact gccgctagca taagcaggag ttgccagccg
    16621 cctttgcgta acatgaccag taatgtggat aaacccaata gcgccagata cagcgcacca
    16681 aagataattt cccgactcag ccatgaactg gaaaagtggc tcagcgcatg catcgcatta
    16741 agtggataac ccaggtgtaa ggtagaaatc agtaacccta cggcacctgc caaaaaagcc
    16801 aacagtaatg gcggccgcat aaccgggcgg gcgctggagg tgattaaaat atgacgatga
    16861 agaaacgcca gccacaacgt cataccgacc gatgcctgta acaacagcgt aaaagccatt
    16921 aacggtaatt catgcatgat gtgacgcctc cttttccgcg ccctgatgcg gtttaatcac
    16981 cagatttggc tgcgtaatcg acgaatcagg taaacctttc acatcactga catcgccata
    17041 cttttcacgc aactcatcaa tagggccgaa cttaatcgcc cccaacgggc acgttgccac
    17101 gcaaatgggt tgttctcctt tagcttgcag atcaatacag aaatcgcatt tcgacatctg
    17161 cccggtctgc tcattcattt gtggtgcgcc gtaaggacag gaccaggcgc agtaaccgca
    17221 accgacgcac ttatcagtat tcacgcgcac aataccgtct ccgggacgct tatgcatcgc
    17281 catcgtcggg caatttttgg tacagatcgg atccgcgcaa tggttacagg aaacagaaag
    17341 ggtataagca aagacattat gactcacacc gccctctcca gtggcgatga agccacctcc
    17401 gttcacttca tacacgcggc gaaaacgtcg cccaacctca aggttgtttt tgtctttaca
    17461 ggccacctga cacgctttac agcccgaaca acgcgaagag tcaataaaga accctaattg
    17521 tttgtcgctg acagatgcaa attctttaaa ttggctcatg ctttctcaac ctccaccagc
    17581 attgtttgat gcgaattccc tttcgccagc gccgtaatgc gggtcgaact cagtacattg
    17641 gcacagcctc cgcgatcgat accttgtgca tccggttgcc accatgcccc cgcctgcata
    17701 gcaactacgc cagggatgat acggggggtt atttccaccg ggataatgct cacaccacga
    17761 tcgttataaa tttttacgct atctccttcg ctaatcccgc gctgttttgc atcttgcgga
    17821 ttcagccaga gtttttgcgt ctgaacttct tgcagccacg ggttagcata ctgggtggaa
    17881 ttagcacggt ttttcccttt ccaggtgatg agctgcaacg ggtatttcgc cgtcagttta
    17941 tcttctggcc cttcaaaggc agggacatag tgcgaaagcg cgggaatttc tggatcttgc
    18001 atatcaaaaa gacgcttaga gaagatctca attttgccgg aaggcgtcgg gaaaggatta
    18061 ttctgcggat cgcgaatatt tgcttcgaaa gcgatgtgcg gcgcgctttt gaaaagatgt
    18121 cgacgtgttt tttgcagggt tgcgaagtcc ggcaaatttt catccggcat cgccagacga
    18181 gtttgctccc agatatgctc gatccattgc ttctcatcac gtccttgact gaactcggct
    18241 tcgacaccca gttttttcgc cacgtcacgc aaccagtcat aatcggtgcg acgttcaaaa
    18301 tccggctcaa tcggtttttc tgacagaata agatagctcg cagttcccca tgtttcgccg
    18361 atattccagc gttccataaa gctggtttcc ggcagcaaca catcggcata tttcgcgctc
    18421 ggagtcatga acagatcgct gagtacgatg aattctattt tagattcgtc ttcaaggagc
    18481 ttcgcggcct ggtggacgtc cgggttctgg ttcgccagat aattacctgc cagtgagaac
    18541 agcaggcgga tattactgtc gagtttatcg acacctttaa gcccatcttg cggcgtaact
    18601 ttagaagcat catccgccgc ctgcatccag ttcataatcg agattttcgc ttgtaccgga
    18661 ttttctggca tatctggtcc aacgcagaat ttacgattag aactaccgcc gtaacctgcc
    18721 gcccagccac ctttgatgcc gacgttaccg gtaatcgagg ccagtaaagt tgaacctcgg
    18781 gcggtgcgtt cgccgcaaat atgtcgctgt ggcccccatc cctgaatcaa cgctgcgggt
    18841 tttgtagtgg cgtaatcgcg tgccagttga cgaatggatt gtgctggaac gtgggtaatt
    18901 ttttctgccc attccggcgt tttgtgtatc ccatcttttg cgccaaaaag gtaggcaacc
    18961 aacgattcgt tggctggtac gccttccggc atggagtttt catcaaaacc gagagtatat
    19021 gtgtcgataa atgtcttatc gtggagattc tcgctgataa tgacgtacat catcgcgtcc
    19081 atcaacgcat tgtccgtggt cggcaacagc gggatccact gatcggcaag tgaagatacg
    19141 gtgtcggaat agcgcggatc caccacgata aaacgtgtac cgttttgctt cattttttga
    19201 aaatagtaat tggtgtgacc gaatatggtt tccgttgggt tatgccccca gagaatcacc
    19261 agtttggtat ccagaagagt at
//
LOCUS       DAZFKY010000082        19156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFKY010000082 DAZFKY010000000
VERSION     DAZFKY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(218..1867)
                     /gene="pgi"
                     /locus_tag="ACHKCL_002588"
     CDS             complement(218..1867)
                     /gene="pgi"
                     /locus_tag="ACHKCL_002588"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5763540.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2104..2300
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2392..3741
                     /gene="lysC"
                     /locus_tag="ACHKCL_002589"
     CDS             2392..3741
                     /gene="lysC"
                     /locus_tag="ACHKCL_002589"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5763541.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3994..4266
                     /locus_tag="ACHKCL_002590"
     CDS             3994..4266
                     /locus_tag="ACHKCL_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5763542.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4267..5139)
                     /gene="rluF"
                     /locus_tag="ACHKCL_002591"
     CDS             complement(4267..5139)
                     /gene="rluF"
                     /locus_tag="ACHKCL_002591"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5763543.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5487..6434
                     /locus_tag="ACHKCL_002592"
     CDS             5487..6434
                     /locus_tag="ACHKCL_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5763544.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6504..7310
                     /locus_tag="ACHKCL_002593"
     CDS             6504..7310
                     /locus_tag="ACHKCL_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5763545.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7320..7727
                     /locus_tag="ACHKCL_002594"
     CDS             7320..7727
                     /locus_tag="ACHKCL_002594"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5763546.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7727..8221
                     /locus_tag="ACHKCL_002595"
     CDS             7727..8221
                     /locus_tag="ACHKCL_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5763547.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8287..9084
                     /locus_tag="ACHKCL_002596"
     CDS             8287..9084
                     /locus_tag="ACHKCL_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5763548.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9095..9919
                     /locus_tag="ACHKCL_002597"
     CDS             9095..9919
                     /locus_tag="ACHKCL_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5763549.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9971..11200
                     /gene="sorE"
                     /locus_tag="ACHKCL_002598"
     CDS             9971..11200
                     /gene="sorE"
                     /locus_tag="ACHKCL_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5763550.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11583..12272
                     /gene="pepE"
                     /locus_tag="ACHKCL_002599"
     CDS             11583..12272
                     /gene="pepE"
                     /locus_tag="ACHKCL_002599"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5763551.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12363..13994)
                     /locus_tag="ACHKCL_002600"
     CDS             complement(12363..13994)
                     /locus_tag="ACHKCL_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5763552.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14214..17897)
                     /gene="metH"
                     /locus_tag="ACHKCL_002601"
     CDS             complement(14214..17897)
                     /gene="metH"
                     /locus_tag="ACHKCL_002601"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5763553.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18097..18921
                     /gene="iclR"
                     /locus_tag="ACHKCL_002602"
     CDS             18097..18921
                     /gene="iclR"
                     /locus_tag="ACHKCL_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5763554.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4516 a         4984 c         5034 g         4622 t
ORIGIN      
        1 ggatttatct gataaaacaa tgccgactta tagtcggcat tgtttttaag gttatttaat
       61 taatcgtgct atgcattaat tgccgaatac gatgtacata tcggcatcga cctgtaggcc
      121 tgataagacg cgtaaagcgt cgcatcaggc accagttgcc ggatgcggcg taaacgcctt
      181 atccggccta catagcggca tcaggcatga acgatgatta accgcgccac gctttatagc
      241 ggttaatcag accattggtc gagctatcgt ggctgctgat ttctttatca tctttcagct
      301 ctggcagaat acggttcgcc agctgtttac ccagttccac gccccactgg tcgaaggtga
      361 agatgttcag gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgcac
      421 ccaggctgaa cggagtgatt tcgcgcaaca ggatagagtt ggtcgggcgg ttaccttcga
      481 acactttgaa cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt
      541 cctgctcaac cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt
      601 tagacagcag tttctggtgg tgatcagaga gcgggttatg ggtgatagcc ggagcgatga
      661 aatcgcacgg taccattttg gttccctggt ggatcagctg gtagaacgcg tgctgaccgt
      721 tagtgcctgg ttcaccccag ataatcgggc cagtctggta atccacaacg ttaccgttac
      781 ggtcaacata cttaccgttg gactccatat tgccctgctg gaagtacgcc gcgaaacggt
      841 gcatatactg gtcatacggc agaatcgctt cagtttccgc accaaagaaa ttgttgtacc
      901 agatgccaat cagcgccaac agtacaggca ggtttttctc ggcaggcgtg gtggagaaat
      961 gcttgtccat cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga
     1021 gaacaatcga caggccaatc gctgaccaca aagagtaacg accgccgacc cagtcccaga
     1081 actcgaacat gttggcagta tcaataccaa actcgccaac ggctttggca ttggttgaga
     1141 gcgccgcaaa gtgtttcgca acgtgttttt catcacctgc cgctttcagg aaccagtcac
     1201 gcgcgctatg ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga
     1261 acagcgtggt ttccgggttt acttttttca gcacttcagc gatgtgagtc ccatcgacgt
     1321 tagaaacaaa gtgcatgttc aggtgatttt tgtacggacg cagtgcttcg gtcaccatgt
     1381 acgggccgag gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gctttgccgg
     1441 tataaccctt ccactcaccg gagataatcg cttccgagaa ggttttcatc ttctccagca
     1501 ccgcgttgac ttctggcata acatctttgc catcaaccag aatcggggta ttgctacgat
     1561 tacgcagcgc tacgtgcagc acggcgcggt tttcagtgcg gttgatcttc tcgccagaga
     1621 acatcgactt aatcgcgccc gccagatcgc actctttcgc cagatcttgt aatttcgcca
     1681 gcgtctcttc agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg
     1741 cggagaactt agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt
     1801 tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt
     1861 ttttcattag caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca
     1921 gtcacagaaa aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag
     1981 cgcagtgaag catttattag ctgaactact gaccgccagg agtggatgaa aaatccgcat
     2041 gaccccgtcg ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta
     2101 gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat
     2161 aacacctgag ggggtgcatc gccgaggtga ttgaacggct ggccacgttc atcatcggct
     2221 acaggggctg aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt
     2281 ggtggagcac ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc
     2341 gctcttccct tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa
     2401 attgttgtct ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc
     2461 gctgatattg tgctttctga tgccaacgtg cgtttagttg tcctctcggc ttctgctggt
     2521 atcactaatc tgctggtcgc tttagcggaa ggactggaac ctggcgagcg attcgaaaaa
     2581 ctcgacgcta ttcgcaacat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt
     2641 atccgtgaag agattgaacg tctgctggag aacattactg ttctggcaga agcggcggcg
     2701 ctggcaacgt ctccggcgct gacagatgaa ctggtcagcc acggcgagct gatgtcgacc
     2761 ctgctgtttg tcgagatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt
     2821 aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg
     2881 gaactggccg cgctgcagct gctcccacgt ctcaatgaag gcttagtgat cacccaggga
     2941 tttatcggta gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat
     3001 acggcagcct tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc
     3061 ccgggcatct acaccaccga tccacgcgta gtttccgcag caaaacgcat tgatgaaatc
     3121 gcgtttgccg aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg
     3181 ttgctacccg cagtacgcag cgatatccca gtctttgtcg gctccagcaa agacccacgc
     3241 gcaggtggta cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg
     3301 cttcgtcgca atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggt
     3361 ttcctcgcgg aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc
     3421 acgtcagaag tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat
     3481 acgttgctga cgcaatctct gctgatggag ctttccgcac tgtgccgggt ggaggtggaa
     3541 gaaggtctgg cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc
     3601 aaagaggtat tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc
     3661 agccataacc tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcaaaaactg
     3721 catagtaatt tgtttgagta aatactgtat ggcctggaag ctatatttcg ggccgtattg
     3781 attttcttgt cactatgctc atcaataaac gagcctgtac tctgttaacc agcgtcttta
     3841 tcggagaata attgccttta atttttttat ctgcatctct aattaattat cgaaagagat
     3901 aaatagttaa gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta
     3961 cactgaactc tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa
     4021 gagatgactg aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg
     4081 catggtcgcg tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa
     4141 ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc agttaaagaa aaagcaggct
     4201 tataaaccgg atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg
     4261 cgctgattag cgccccttct tttttcgccc cggcgaggta aaacgcttac cgttggacgc
     4321 cgggcgaccg cctttttccg ccgttttttc catcttaacg actggacgtt tgatgcccgc
     4381 tgttttcggt ttggccttcg ctttaggttt tgcctcggaa gaggaatttt cgatgagctt
     4441 aaagaggtcg attaactcat cgtcggttaa atcgcgccac tcccccagcg gaatgccgct
     4501 taagctgacg ttcataatgc gcgtacgttc cagctttttc acttcatagc cgaaatgctc
     4561 gcacatgcgc cggatctgac ggttcagccc ctgcaccagg gtaatgcgga agacaaacgg
     4621 cgcttctttt ttgactttgc actttttagt caccgtcccg aggatcggca cccccgcccc
     4681 cataccacga ataaattcat cggtaatcgg tttatcgacc gtcaccagat actctttctc
     4741 atgatcgtta cccgcacgga ggatcttatt caccagatcg ccgtgattgg tgaggaaaat
     4801 cagcccctgg gagtctttat ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac
     4861 gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg ctgacaatgc ctacgggctt
     4921 gttcagggcg ataagtacca aatcttcggc ttcccgaggc tcaatcaact gaccgtttac
     4981 tttcacaacg tcgccgggtt tcacctgatc gccaatggtg gctcgcttgc cattaaggaa
     5041 cacattgcct tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct
     5101 gatgtattta tttaaacgga ctgatgagtc gggcagcata aattctcctg taaaacgcgg
     5161 aatataccgt acctcttagc ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg
     5221 tgatctgcca cgccttttat cacaaaagtg tctccggaag tacaatgcaa atttgcgtgg
     5281 caaaataagg ataatcaaca tattacgaat cgctgcacac ctgtgctgat aactactgcg
     5341 cagagaaaca aatgtgcaag agaaagcgct atttctcccc atgcaaagag tgaaaaatcg
     5401 ctttgaaagg ccatcatgat ggggttatat cagatcaagc ggtgagaaag cgtatttgca
     5461 caaatgtgca gaaccaggag gcggcaatgg aaaacagtga cgatatccgt ttgattgtga
     5521 agattgccca actctattac gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg
     5581 gtatttaccg caccaacatc agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca
     5641 ccatcgccat caactatgac tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa
     5701 agtttggcct gaaagacgtt gtggtggtgt cgggaaatga tgaggatgaa gagactcaac
     5761 tggcgatgat ggggttacac ggcgcgcaac tgctggatcg cttgctggag cctggcgata
     5821 ttgtcggttt ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg
     5881 ggcaatcgcg gcagttaatc tgcgtaccaa ttattggcgg accgtccggt aaactcgaaa
     5941 gccgctatca cgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc
     6001 atctcgcgga ttttccggct ctactggata acccattaat tcgtaatggg atcatgcagt
     6061 ctcagcactt taaaaccatc tctgcctact gggataatct ggatgtcgcc ctggtgggaa
     6121 ttggctcacc ggccattcgc gacggcgcta actggcatgc gttttatggt ggtgaagaga
     6181 gtgacgacct gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt tttgatattc
     6241 acggcgaaat ggttgaaacg aatatgagcg aaaaaacact ctctatcgaa atgaataaat
     6301 taaagcaggc acgatattcc attggcattg ccatgagtga agaaaaatac agcggaatta
     6361 ttggtgcact gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac
     6421 tattactgaa ataaaaatct tcccgatgtt tatcgtaata aaaattacgg cgggtcccta
     6481 ttatctaaaa tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta
     6541 tcgtcaccgg cggcgcatcc ggtattggtc tggcgattgt agaggaatta ttagcacaag
     6601 gcgcaaacgt acagatggta gatattcacg gtggcgatgg acaatatgaa ggtcataaag
     6661 gctatcagtt ttggccgacc gatatttcca gcgccaaaga ggtaaatcat acggtagcgg
     6721 aaattatcca gcgttttggt cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc
     6781 cgcgtctgct ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat
     6841 tcgaaaaaat ggtcaatatc aaccagaaag gcgtttttct gatgtcgcag gcggtggcgc
     6901 gacagatggt caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg
     6961 aaggctcaga aggccaaagc tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc
     7021 gctcctggtc gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc gcgccgggga
     7081 ttctggaaaa aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca
     7141 atatcaccgt cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg
     7201 ccggaagatt agcagaaatc gctgattttg tttgttatct gctgtctgaa cgcgccagct
     7261 atatcaccgg agtaaccact aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta
     7321 tggtcaacgc cattttttgt gcccacggca aattagcttg cgccatgctg gaatcggttc
     7381 agatggttta cggcaacgcc aatgttgaag ccgttgaatt tgtgcccggt gaaaacgccg
     7441 gagacatcgt agcaaagctg gaaaaattag taagcattca caatcaaaat gagtggctga
     7501 ttgccgtgga tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca
     7561 atcctcgtct gcgggtaatc agcggacttt ctctgccgct ggcgctcgaa ctggttgata
     7621 accaggacag catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa
     7681 cctgcgttgt ctggaaacaa ctggcaacga cagaggagga tttctgatga acattacgct
     7741 cgcccgcatt gatgaccgcc tgatccacgg tcaggtcact accgtctggt cgaaagtggc
     7801 taacgcccag cgcattatta tctgcaatga cgaagtttat aacgatgaag ttcgccggac
     7861 attattgcgt caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc
     7921 cgtcgcggtt tatcacaacc cgcaatatca ggacgaaacc gttttttatc ttttcactcg
     7981 cccacaggat gccttagcga tggttcgcca gggggtaaaa ataggcaccc tgaatattgg
     8041 cggcatggcc tggcggcccg gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga
     8101 tgatattaac gcatttcatg agttaaataa tctaggcgtc atactggatt tacgcgtcgt
     8161 agcgtcagat ccctcaatca atattatcta caaaataaac gaacagttaa tcgcaaatta
     8221 aataatagcg cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga
     8281 caaatcatgg aaataagtac cctacaaatc attgctatat ttcttttttc ctgtattgcc
     8341 ggaatgggca gcgtgctgga tgaatttcag actcatcgcc cgttaattgc ctgtacggtg
     8401 attggtttaa ttctcggtga tttaaaaacc ggaataatac tcggtggtac gctggaattg
     8461 atagctctcg gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc actcgccagc
     8521 ataatctccg ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc
     8581 gcgttgcctg tggctgcggc aggccaggtg ctgacggtgt ttgcccgtac catcaccgta
     8641 gtgttccagc acgcggcgga taaagcagcg gaagaagcac ggtttcgcac tctcgatatt
     8701 ctgcatgtct ccgcgcttgg cgtgcaggcg ctgcgcgtcg ccattccggc actgattgtc
     8761 tcgctgttcg tcagcgccga tatggtgagc aatatgttaa acgccattcc cgaatttgtg
     8821 acccgaggtc tgcaaattgc tggcggtttt atcgtggtag tcggttacgc catggtgcta
     8881 cgtatgatgg gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc
     8941 tacctcgatc tcagtctgct ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac
     9001 atccagctaa atccacagtg gcgtaaagct gaacctcaac tcctggcccc cgcttccacc
     9061 gcccttgacc aacttgacga ttaacggagc catcatggaa cagagaaaaa ttacacgcgg
     9121 cgatctggtg agcatgtttc tgcgctccaa cctgcaacag gcgtccttta actttgaacg
     9181 tattcacggg ctgggttttt gctacgacat gatccccgcc atcaagcgac tttacccagt
     9241 aaaagaggat caggttgcgg cgctcaggcg acacctggtg ttctttaata ccacgccagc
     9301 cgtatgtggc ccggtcatcg gcgtcactgc cgccatggaa gaggcgcggg ccaacggcgc
     9361 ggaaattgat gacggcacca ttaacggcat caaagtcggt ctgatgggac cattggcagg
     9421 ggttggcgat ccactggtct ggggaacgct gcgcccgatt accgccgcgc tcggcgcatc
     9481 gctggcgctg tctggcaaca ttctcggccc gctgctgttc ttctttattt tcaacgcggt
     9541 gcgtctggcg atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt
     9601 cagcgatatg ggcgggaatg tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact
     9661 gtttgtgatg ggcgtgctgg tcaccaaatg gacgtcaatc aacgtaccgt tggtggtttc
     9721 acaaacgcat gccgccgatg gctccaccgt caccatgacc gtgcagaaca ttctcgatca
     9781 actttgccct ggtttgctgg cgctcggtct gacgctactg atggttcgtc tgctcaacaa
     9841 aaaaattaac ccggtatggc tgattttcgc cctgtttggc ttagggatta tcggcaatgc
     9901 gctgggcttc ctgtcctgat tcttcgcccc ggcacgactg ccggggccat cgttcaacat
     9961 gaggtggttt atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga
    10021 aacctttgac cttcctgaaa tgcaggagga tgaaatcctc gcgacggtgg tcactgacag
    10081 cctgtgcctc tcttcctgga aagaggccaa tctgggtgaa aaccataaaa aagtacccga
    10141 cgatgtggcg accaacccaa tcatcatcgg ccacgagttt tgcggcgata ttctggccgt
    10201 gggtaaaaag tggcagcaca aattccagcc gggtcagcgt tatgtgattc aggccaacct
    10261 gcaactcccc gaccgcccgg actgccccgg ctactccttc ccgtgggtag gcggcgaggc
    10321 cacgcatgtg gttattccca acgaggtcat ggaacaagat tgcctgctgg catacgacgg
    10381 cgaaacctat tttgaaggct cgctggttga accgctttcc tgcgtgattg gcgcgttcaa
    10441 cgccaactat catcttcagg aaggtagtta taaccacacg atggggattc gcccgcaagg
    10501 gcgcatgctg atcctcggcg gcaccggacc aatgggactg ttggcgattg attatgcgct
    10561 acatggaccc gttaacccgt cgctgctcgt cattaccgat accgacaacg ataaattgag
    10621 ttatgcgcgc aagcactatc catcagaacc gcaaacactg attcattatc tcaatgccgc
    10681 cgatgcagca tttgatacgc taatggcgct gagtggcggt cacggcttcg atgatatttt
    10741 cgtctttgtg cctaatgaag gactggtgac tctcgcctct tccttgctgg cgacagatgg
    10801 ttgcctgaat ttcttcgccg gaccgcagga taaacatttc agcgcgccaa ttaatttcta
    10861 cgatgtgcat tatgcattta cccactacgt gggcacgtca ggcggcaata ccgacgacat
    10921 gcgcgcagcg gtcaaattga ttgaagagaa aaaagtgcag gccgcaaaag tggtaacaca
    10981 tattcttggg ctgaatgccg cgggcgaaac cacgcttgaa ttgcctgccg tcggcggcgg
    11041 caaaaagctg gtgtataccg ggaaatacct gccgctgacg tcactcacgc agattcagga
    11101 tcaagcactg gcggcgattc tggcgcgtca tcaggggatc tggtccggtg aggcggagca
    11161 atatctgctc actcatgcag aggcaatttc ccatgattaa tcgcgctact taactctgca
    11221 tgtcgctggc tggtcaccgc ggcaacttcg cgacctgatg tgcggctgat tgctgaagcg
    11281 gcccaatgtg cacgagaagg ataaaaaaac atcagcgcct taaggggcgc tcaaattggt
    11341 ggcaaagtac acattgttta tgccggatgc ggaataaagc cttattcggc ctacataatc
    11401 tcgaaaattc aatatattgc agttatctgg taggcctggt aagcgtagcg catcaggcaa
    11461 tgctgtgttt gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca
    11521 gtaagtggtt aaattcgcag tggtagcatg ccttccgtga tttcttacta accggagaag
    11581 taatggaact gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg
    11641 cactgccgct gattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg
    11701 ctggcgtaac gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc
    11761 tgggtgtttc tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg
    11821 cggaaattgt gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc
    11881 gcgggctgct ggctccaatt actgacgtgg ttaaacgtgg cgcgctgtat attggctgga
    11941 gcgcaggcgc taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg
    12001 atccgcaagg tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg cacttcacca
    12061 acgcgctgcc ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg
    12121 tcgtcgcgcc agaactgacg attattggtc taccggaagg taactggatc acagtgagta
    12181 aaggtcacgc aacgctgggt ggcccgaata ccacctatgt gtttaaggcc ggtgaagaag
    12241 cggttccgct ggaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat
    12301 cttgttcata gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa
    12361 tattaatact cgtcccgtcc ctcatcttca tcaggctgtt ccagcacact gtaagccacc
    12421 gaacaaaaca gcgagttcag acgctgcata tctcccagta agcctaaatg tagcgaactg
    12481 gtttcaatgc tttgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg
    12541 cgattaagaa tgcgaaagcg atgcttgctg cggcgcaaac gacgagcgct ggtgacatcg
    12601 ccagagaaga acaccgacat tgccagcttt aaattactga gcaattgctc atagagcgca
    12661 tccagttcct tcaagccatc aagcgaaaat gctcgccttg cagccagcga tttgtcggca
    12721 atttcactac tcatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg
    12781 atgatctccg cccagcggcg cgactcttct tctgccagtt cctcttttgg catccgcgcc
    12841 agatacagct taatggcggt atagagaacg ttgatatcat ctgccagctt acgcagctct
    12901 ttctcctgcc ttggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg
    12961 gcgtcgccaa tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc
    13021 agcgcgctga catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga
    13081 ataatcgttt tgcaaaaccg tgccatcggg tcaacaaatg gcagcatggc caggcaacgt
    13141 acaaggttgt agaagacgtg gaaatagatc accagttccg cttttggcaa tgacaacttc
    13201 cccattgtct ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta
    13261 aacagcagac tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc
    13321 atcgccagca ggccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag
    13381 gagataatgc ctgcggcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag
    13441 ctgataatgg cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag
    13501 gcaaagatca cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac
    13561 tccagcgcca gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt
    13621 gactgtttgc gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag
    13681 gataagtcga aggtgagaat acgcgccatt agcgccgtcc cgacatctgc ccccagcaca
    13741 atgaccagag ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg
    13801 gtggtggcat tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc
    13861 ggcttttttt cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt
    13921 acgccggttc gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtta
    13981 agcagcgtta acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat
    14041 gaccaaagga ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt
    14101 tccggctaag attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca
    14161 gggcactcat tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc
    14221 gcgtcatacc ccagattcgg tgccagccag cgctcaacat cggagacgct cataccttta
    14281 cggcgggcat agtcttcaac ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg
    14341 tccgggtggc tgaaatacca acccgaaacc gatgcaccgg gccacatggc gaaagattct
    14401 gtgagtttca tgccggtatg tttttccact tccagcagct cccagatggt ggctttttcc
    14461 gtatgttccg ggcaggccgg gtagcccggt gccggacgga tgccctggta gttttcgcgg
    14521 atcagttctt cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca
    14581 cgctcatgga gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg
    14641 attttgttgt aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc
    14701 ccgccagtca cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg
    14761 aagtcagcga gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg
    14821 ctgacgttga tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca
    14881 cggtttgccg ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat
    14941 ttatccagca tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca
    15001 tcttccagaa tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag
    15061 tcgatgtaat tacgcagagt ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc
    15121 gccaccggcg gcgtgtaagc ctgccagtca aaagcgaagt cgttatcgcg cgccgcttcc
    15181 agcgtgaccg gtggtgtgcg tggtttcttg cgcccgtgct gaatacgtac ggtttcgtac
    15241 tccttgcggg tacgagcgac aaaatcatcg cgctgagtat cggaaagcag cgccgccacc
    15301 acaccaacgg tgcgcgaggc attctgcaca tacaccgtcg ggccgctgta gttctgctcg
    15361 attttcaccg ccgtgtgcgc ttttgaggtc gtcgcgccac cgatcagtaa cggaatggtg
    15421 aagccctgac gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata
    15481 agccccgaaa gtccaatcag atcagcattc acttctttag cggtacggag aattttttcc
    15541 gcaggcacca taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacacca
    15601 acgatatttt taccgatgtc gtggacgtcg cccttcacgg tggcgatcac catcttgccg
    15661 ttggttttgc cctgctcttt gctggcttca ataaacggtt cgaggtaggc cactgcctgc
    15721 ttcatcacac gcgccgattt gaccacctgt ggcaggaaca ttttcccttc gccgaacaga
    15781 tcgccgacca cgttcatgcc gtccattagc gggccttcaa tcacttcaat cgggcgcgta
    15841 gcctgctggc gggcttcttc ggtatcctgc tcgataaact cagtaatgcc tttgaccagc
    15901 gagtattcca gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttagcg
    15961 gtgtcgtcgg ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca
    16021 tcatcgcgac gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg
    16081 tcgtaaatcg ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg
    16141 tagtagagga acaccgcgtg aatggcttcg cgcaccggat cgttgccacg gaatgagaaa
    16201 gaaacgttag atacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttcg
    16261 caggcgccga taaagtcctg cgcatagttg ttgtgctctt caatgccggt ggcgaccgcg
    16321 aagatgttcg ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt
    16381 ttgtacgccc gacggcaaat ctcaattttc cgtgcgcggg tatcggcctg cccctgttcg
    16441 tcaaaggcca ttaccaccac cgctgcaccg taacgacgca acagtttcgc atgatggata
    16501 aaggcgtcga cgccctcttt cattgagata gagttaacaa tgcctttgcc ctgaatacac
    16561 ttcagcccct tttcgatgac ctcccatttt gaggagtcga tcataatcgg cacgcgagcg
    16621 atatccggtt caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcatcgagc
    16681 atcccttcat ccatgttgat atcgataatc tgcgcgccgt tttccacctg ctgacgcgct
    16741 acatccagcg cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg
    16801 gtgacgttgg tgcgttcacc cacgttcaca aacaggctat cgtcgccaat gttcagcggc
    16861 tccaggccgg acaaacggca ggctacggga atttcaggca gtttgcgcgg cgctaatcct
    16921 tctactgcac gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg
    16981 atattgagaa aacccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg
    17041 agatcgtact caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat
    17101 tccgcaatcc gtgatagttc ctgcacgtac tggcgcagtt catctggccc cagcgcgcag
    17161 ttgaggccaa aagtcagggc ttcggcatgg cgcagggagt tgtaaaatgc ttcggtggtc
    17221 tgtccggaga gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc
    17281 tcaacgccta acgcttcgaa ctccgttttc accgcaaaga ccgccgcttt ggcgttaagg
    17341 gtgtcgaaaa cggtttcaat cagaatcaga tccgcaccgc cttccactaa tgctttggtg
    17401 gattcgcgat aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttc
    17461 acgtccggag aaatggacgc cgtgcggttg gttgggccga gaacgccggc aacgtagcgc
    17521 ggtttctccg gcgtgcgcgc ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc
    17581 gcaaagttga tttccgccga cagggattcc atctggtaat ccgccatcgc aatggtcgtg
    17641 gagttgaagg tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata
    17701 gcggcgatca cttccggttt gcttagcacc agcagatcgt tattgccttt gaggtcgcat
    17761 ggccagtcgg caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc
    17821 atggtgccca taccgccgtc cagcaccaga atacgttcat ttaactgcgc acgcagttgt
    17881 tccactttgc tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa
    17941 caaaccatac tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt
    18001 gttcaacatt aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg
    18061 aaaatgattt ccacgataca gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa
    18121 cgcggcagaa aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg
    18181 cgtggcctga aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacggag
    18241 ctggcgcaac aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa
    18301 cagcagggtt tcgtgcgtca ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc
    18361 tttatggtcg gcagcagctt tctccagagc cgtaatttgt tagcaattgt tcaccctatc
    18421 ctgcgcaatc taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc
    18481 gatcacgaag cgattattat cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg
    18541 cctatcggcg gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa
    18601 ctgagcgaag aacaggtgac gaagctgctg caccgcaaag ggttacatgc ctatacccac
    18661 gcaacgctgg tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt
    18721 tattcatttg acgatgagga acatgcgctg gggctacgtt gccttgcagc gtgtattttc
    18781 gatgagcacc gtgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc
    18841 gatgaccgcg tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg
    18901 gcgtacggtg gaatgcgctg actttttctg gcgggcagag gcaatattct gcccgtcata
    18961 cctgagtggt aatagaataa gggtgtctgt taatcgcatt gacgtcaaaa taacttattg
    19021 tcatacactt catacacttt tattaactca gcattatttt taaacatcaa accacttaat
    19081 tattaacaaa atcgcaagat ctcattccac cgaaactttt acggctgcat tatgagattg
    19141 agcgatttat gatatt
//
LOCUS       DAZFKY010000083        19068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFKY010000083 DAZFKY010000000
VERSION     DAZFKY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..735)
                     /locus_tag="ACHKCL_002603"
     CDS             complement(<1..735)
                     /locus_tag="ACHKCL_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5763555.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANI"
     gene            743..1174
                     /gene="tnpA"
                     /locus_tag="ACHKCL_002604"
     CDS             743..1174
                     /gene="tnpA"
                     /locus_tag="ACHKCL_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO5763556.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            complement(1232..1525)
                     /locus_tag="ACHKCL_002605"
                     /pseudo
     CDS             complement(1232..1525)
                     /locus_tag="ACHKCL_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1797..2591
                     /gene="yjfZ"
                     /locus_tag="ACHKCL_002606"
     CDS             1797..2591
                     /gene="yjfZ"
                     /locus_tag="ACHKCL_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5763557.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2662..3111)
                     /gene="rplI"
                     /locus_tag="ACHKCL_002607"
     CDS             complement(2662..3111)
                     /gene="rplI"
                     /locus_tag="ACHKCL_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5763558.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(3153..3380)
                     /gene="rpsR"
                     /locus_tag="ACHKCL_002608"
     CDS             complement(3153..3380)
                     /gene="rpsR"
                     /locus_tag="ACHKCL_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5763559.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3385..3699)
                     /gene="priB"
                     /locus_tag="ACHKCL_002609"
     CDS             complement(3385..3699)
                     /gene="priB"
                     /locus_tag="ACHKCL_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5763560.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3706..4101)
                     /gene="rpsF"
                     /locus_tag="ACHKCL_002610"
     CDS             complement(3706..4101)
                     /gene="rpsF"
                     /locus_tag="ACHKCL_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5763561.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4428..4703
                     /gene="yjfY"
                     /locus_tag="ACHKCL_002611"
     CDS             4428..4703
                     /gene="yjfY"
                     /locus_tag="ACHKCL_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5763562.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4832..5518)
                     /gene="ulaF"
                     /locus_tag="ACHKCL_002612"
     CDS             complement(4832..5518)
                     /gene="ulaF"
                     /locus_tag="ACHKCL_002612"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5763563.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(5518..6372)
                     /gene="ulaE"
                     /locus_tag="ACHKCL_002613"
     CDS             complement(5518..6372)
                     /gene="ulaE"
                     /locus_tag="ACHKCL_002613"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5763564.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6382..7032)
                     /gene="ulaD"
                     /locus_tag="ACHKCL_002614"
     CDS             complement(6382..7032)
                     /gene="ulaD"
                     /locus_tag="ACHKCL_002614"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5763565.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7046..7510)
                     /gene="ulaC"
                     /locus_tag="ACHKCL_002615"
     CDS             complement(7046..7510)
                     /gene="ulaC"
                     /locus_tag="ACHKCL_002615"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5763566.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(7520..7825)
                     /gene="ulaB"
                     /locus_tag="ACHKCL_002616"
     CDS             complement(7520..7825)
                     /gene="ulaB"
                     /locus_tag="ACHKCL_002616"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5763567.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(7841..9238)
                     /gene="ulaA"
                     /locus_tag="ACHKCL_002617"
     CDS             complement(7841..9238)
                     /gene="ulaA"
                     /locus_tag="ACHKCL_002617"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5763568.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9593..10657
                     /gene="ulaG"
                     /locus_tag="ACHKCL_002618"
     CDS             9593..10657
                     /gene="ulaG"
                     /locus_tag="ACHKCL_002618"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5763569.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            10765..11520
                     /gene="ulaR"
                     /locus_tag="ACHKCL_002619"
     CDS             10765..11520
                     /gene="ulaR"
                     /locus_tag="ACHKCL_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5763570.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(11517..12266)
                     /gene="yjfP"
                     /locus_tag="ACHKCL_002620"
     CDS             complement(11517..12266)
                     /gene="yjfP"
                     /locus_tag="ACHKCL_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5763571.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12448..12777
                     /gene="bsmA"
                     /locus_tag="ACHKCL_002621"
     CDS             12448..12777
                     /gene="bsmA"
                     /locus_tag="ACHKCL_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5763572.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            12926..13201
                     /gene="yjfN"
                     /locus_tag="ACHKCL_002622"
     CDS             12926..13201
                     /gene="yjfN"
                     /locus_tag="ACHKCL_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5763573.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13318..14943)
                     /gene="aidB"
                     /locus_tag="ACHKCL_002623"
     CDS             complement(13318..14943)
                     /gene="aidB"
                     /locus_tag="ACHKCL_002623"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5763574.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15027..16190)
                     /gene="yjfC"
                     /locus_tag="ACHKCL_002624"
     CDS             complement(15027..16190)
                     /gene="yjfC"
                     /locus_tag="ACHKCL_002624"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5763575.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(16193..16831)
                     /gene="yjfM"
                     /locus_tag="ACHKCL_002625"
     CDS             complement(16193..16831)
                     /gene="yjfM"
                     /locus_tag="ACHKCL_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5763576.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(16841..17239)
                     /gene="yjfL"
                     /locus_tag="ACHKCL_002626"
     CDS             complement(16841..17239)
                     /gene="yjfL"
                     /locus_tag="ACHKCL_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5763577.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17257..17916)
                     /gene="yjfK"
                     /locus_tag="ACHKCL_002627"
     CDS             complement(17257..17916)
                     /gene="yjfK"
                     /locus_tag="ACHKCL_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5763578.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17967..18665)
                     /gene="yjfJ"
                     /locus_tag="ACHKCL_002628"
     CDS             complement(17967..18665)
                     /gene="yjfJ"
                     /locus_tag="ACHKCL_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5763579.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(18684..>19068)
                     /gene="yjfI"
                     /locus_tag="ACHKCL_002629"
     CDS             complement(18684..>19068)
                     /gene="yjfI"
                     /locus_tag="ACHKCL_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5763580.1"
                     /translation="ALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4863 a         5016 c         4529 g         4660 t
ORIGIN      
        1 gatatttgcg atacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttgtt
       61 gctgaatttg accttgcggc ttaactgtcg ctgaagtctc gccagcgtct tctggttttt
      121 ctgaaaactg tttacaggtc caaagactgt gccatctgac agcgtggcga gtttagccac
      181 gccagcatcc agtccgacca ttaatgctga agggtgaacc ggagttgata cttcattttc
      241 agtctgaata ctgatgtacc acttaccgca ggactggctg gcagtgacat ttttcacaac
      301 acccgtgacc tgacggctat tccggtagcg catccagcca agtttcggca gaaaaatacg
      361 gctgttttcc tgatcgagct taacgccctg cgggtagcgg aatgcatcat tctgtccccg
      421 ctttttgaat cggggaaaag cagcccgctt ccggaagaag tttttgtaag cccgctcaag
      481 gtctttcagt gactgttgca atggctgtga cggggcatct ttaagccatt gcgtttcagt
      541 ggcatttttc cactcaacca gccaggaagc cattttcccg taagggatgt atttattacc
      601 ggcctcatga ttctcattct gaagcgccag agcacgattg aaaacgaaac gacatgcgcc
      661 tgcgaagcgc ctcatttcac gctcctgttg accaccgggt cttaactgga atttaaatgc
      721 ctgtagtcgt ttcacaggcc aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg
      781 gcgtggaagg cattgtgttt tcctgatgca tgttcacctg gtctttgtca ccagataccg
      841 acgccagatt tttgaccatg acgcgacaga aaaactacgc acttactttt caaaggtatg
      901 tgctgatttt gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatttgtt
      961 aattaactat cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc
     1021 gggtaggtta ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt
     1081 tttgtggagt cctggctatt ttgccagtag ctgcggaggt gcgccaatat ccgccatccg
     1141 tcaatacatt gaacaacagc aaacacctgg ttaggtggaa aaccgcgccg tatatccccg
     1201 ccctgaagga cggggtttta cggcgcaccg gataaaccag gccaagcgta tgctggttga
     1261 tcaaataata tcccccaagc gcagcaatat taatattcac ctgcaatggg tcgatatctg
     1321 gcttaaaaat attgtatttc ttcccttcat ccaataagga ttccattaac tgcaagtgcg
     1381 catggttgat ttctaagaga cgctgtgatt ttgcataatg cacaccttta ctttgatttt
     1441 cactatgcac aatttttaaa aaccaggggt tagcaatata ataatcccat gtaaaatcaa
     1501 taattttctc aatggctttt tgtggctggt taacctgctg attaacgaat acaccgatac
     1561 cgacagcgta gaataattac ataccgcaca acaagaatgt acggtgtaag gcgtggaagt
     1621 cattttctcc ccgggtgcat cgttatatcc ataatgagcg atttttgata ttaatatctt
     1681 tcaccatccg ctattttcac cacttcagaa aacaaactta tttttattaa tatcgatttt
     1741 tttctgtgaa cattcagctg cgatatttat aaaaacatta aacaggtcac attacaatga
     1801 cattaccaac cactatttat tcatttcctg catatttatc tcgtttttcg tccaccgaca
     1861 aaccagtaaa attaaaattt catcaatacg cacgcgctac attactttca aatcgtggaa
     1921 gagatcataa ttgcgatggc agacgtactg ttgagatcca caaactagat ctctcagact
     1981 ggcaagcatt caataagtta gcaacgaagt gtaatgctta cgatggagta acaatgaatg
     2041 gtgataattc ttttggatgg aaccatgaag ctacattaga taatattcac gcccagaaat
     2101 acaataaagc ttacgcgggt gcgaggttaa ccgcagaact gaaatattta ttgcaagatg
     2161 tcgaatcctt cgaacccaac agtaaataca caatacacga agtggttttg ggacctggtt
     2221 atggtactcc agactatacc ggccaaacga taggatacgt tgtaaccctt cctgcccaaa
     2281 tgcctaattg ctggtcatct gaactaccaa caatcgatct ttatatcgac caacttcgca
     2341 ctgttacagg cgtttctaat gccctcggat tcatcattgc tgcattgcta aacgcgtatt
     2401 ctgatttacc ccatgatttg aaaattggtc tgcgttcctt atcaagttca gccgcaatct
     2461 actcgggttt gggtttcgag cgagtgccgc aggagcgaga catcagttgc gctcgtatgt
     2521 atctaacgcc agcaaaccat cctgacttat ggacacaaga gaatggtgaa tggatctact
     2581 tgcgaaacta atacctgacg aaaaatagaa agcaaaacgc cgaccagtgg tcggcgtttt
     2641 tacgtctcgt tgaataacga attattcagc tactacgttt acgatcactt tcgcgaatac
     2701 ttcgctgtga acctggaagc tcacttcgtg ttcgccagtg gtacgcagaa cgccgttcgg
     2761 cagacgaact tcgctcttag ccacttcaac gccagctgca gttacagcgt cagcgatgtc
     2821 gcgagtaccg atggaaccga acagtttacc ttcgtcgcca gctttagacg cgatggtaac
     2881 agtttccagt gcattgattt tctcagcgcg agcattagca gctgccagaa cttcagccag
     2941 tttagcttcc agttcagcgc gacgtgcttc gaagaattca atgtttttct tggtagctgg
     3001 aacagcttta ccctgtggta ccaggaagtt acgagcatag cccgctttaa cgtttacctg
     3061 atcacccagg ctacccaggt ttgctacttt atcaagcaga ataacttgca ttaccttatc
     3121 ctctcaaagt cgtattaatg gaccgtgacc gattactgat ggcgatcagt gtacggcagc
     3181 agggacaggt agcgagcgcg tttgatagcg cgagccagct gacgctggta ttttgcacgg
     3241 gtaccggtga tacggcttgg gacaatctta ccgctttcgg tgatgtagtt tttcagcgta
     3301 gcgatatctt tatagtcgat ctcttgaacg ccttccgcgg tgaaacggca gaacttgcga
     3361 cgacggaaat aacgtgccat atggctagtc tccagaatct atcaattcaa tctgctcggc
     3421 atgcaaaacc attttgctca gtccgttctt tgccttgtgg catgaaatga acccctgaac
     3481 ggttatgcga ctgccgaccg ttatactgtg agtaatggcc tggttttcgt gtccgctaac
     3541 aataacgggc atttgacacc atgcctgccg gtgaaagccg gcttcctcct gcacagaacg
     3601 atgctcaagc acgaactggc agtgaggaat tcctgatgga ctgacctttc gaaggggagt
     3661 cctgcacacg gtgccggaca acaccagacg gttggtcatc agaaattact cttcagaatc
     3721 cccagcttca gcatcatcag cggtttcgtt tgcgaaatca tcgcgacgct cacggcgctc
     3781 gtctttcgct ttaaccatcg gagatgcttc ggtaacagcg tgcttggtac gcataaccat
     3841 gctgcggata acggcatcgt tgaagcggaa ggtagtttcc agctcatcga tcacttcctg
     3901 cggagcttca acgttcatca gaacgtagtg agctttgtgc agtttgttga tcgggtaagc
     3961 cagctgacgg cggccccagt cttccagacg gtggatcttg ccttctgcac cagtgatggc
     4021 agcagtgtag cgctcgatca tgcccggaac ctgttcgctc tggtcaggat ggaccataaa
     4081 aacgatttcg taatgacgca tcgaattgct ccttacggat tattcagcct cctgtctggg
     4141 tcagccgaat cccggggagg caaggaacgt gttaaaggtc ggctgaaaaa tgacgcgtta
     4201 tagtactgac ataccccgct aaactcaagg ccagccgaca aataaatctc atctttcacc
     4261 aaagtggact aacacgctga tttgaagttc atcacacttc atcatggtgt tttttcgaac
     4321 aactgcatca gaaatggtca ccatcaactt ctttatcatt gattaaaatt aaatcagaag
     4381 atcatcacag aggagagcac acaaagtcag ccgtcgagga ggtatctatg ttcagtcgag
     4441 ttttagccct tctggctgtg cttttgctaa gtgcaaatac atgggcagcc attgaaatta
     4501 ataaccacca ggccagaaat atggacgatg tgcaaagctt aggcgtgatt tatatcaatc
     4561 ataatttcgc cactgaaagc gaagcacgtc aggcattaaa tgaagaaaca gatgcgcaag
     4621 gcgcaacgta ctaccacgta attctgatgc gggaaccggg gagtaacggc aatatgcacg
     4681 ccagcgcgga tatttatcgc tagcaccagg tataaccaac gaaacattgc catagtttgc
     4741 tttgccccct tcgcaggggg ctttttttga aaaacggctg cggaattaga ccagttatct
     4801 cccgaggaag gaaatttccg cagcgcgtgt tctacttctg cccgtaataa gcgttggggc
     4861 cgtgcttacg catgaagtgt ttattcatca ggaagctgtc gatgtgattg agttgtggat
     4921 taatactgcg cgcaatccac gccattttcg ccacttcttc catcactacc gcgttatgca
     4981 ccgcgtcgtg ggcatctttc ccccaggcga acggtccgtg ctgatacacc acaattcccg
     5041 gcgtatgcag cggctcggcg ttgcccagcg tttcgataat cactttgcca gtgttcagtt
     5101 catactcacc ctgcacctct tcttcgctta atccacgcgt acacgggata tcaccaaaga
     5161 agtagtcagc gtgcgtggtg cctaacgccg ggatcgccag ccccgcctgc gcccatgcgg
     5221 tagcatgagt ggagtgggta tggacaatgc cgccaagcga cggataacga cggtagagtt
     5281 cgagatgcgt cgcggtgtcg gaagacggac gatattcccc ttccaccacc ttaccgctca
     5341 tatcgaccac caccatatcg tctgctttca tggtttcgta ggcgacgccg ctgggcttga
     5401 tcaccaccag cccgcgttcg cggtcgatag cgctgacgtt gccccaggta aaggtcacca
     5461 gcccatagcg cggcagatcc atgttggctt caaacacctg ctgttttagc ttttgcatta
     5521 tgccgcctcc accatgcccg ctttcgccat gcgcgctttc acccaatcac gcgctttcgc
     5581 cacttctgcc gccgggtctt ccgccgtttc gctccacatc tcaatcaggt acggcccgca
     5641 atagccactc tgtttgagcg tttcgaagca acgttcgaaa tccaccacac cttcgccaaa
     5701 tggcacgttt ttgaagacgc ccggtttggt gtctttcaca tgtaccgcga cgatatgccc
     5761 gattccggcc tgcaactcca tctgcacatc gttgtcccac gccgacaggt tgccgatatc
     5821 cgggtagagc tggaaccacg gattgttgag atagtgcgcg tagcccagcg ccttgctgat
     5881 ggagttcatc aacggataat ccatgatctc catcgccagc gtcacctgcg cgcggctcgc
     5941 catctcaacg ctctctttca ggccgtcacg gaaacgacga cgcgtttcgt tattggcttc
     6001 ctgatagtaa acgtcatagc ccgccagttg gatcacgcga ataccaacgt cctgggcgaa
     6061 ctggatagct ttacgcataa tctccagccc ctgcgcccgc accgcgtcat cttcactgcc
     6121 cagcgggaaa cgacgatgag cagaaaggca catggacggc acgcgcacgc cggtttcaac
     6181 aatcgcattc accagcgcca gacgctgctc gcggctccag tcgaggcgcg acaggcgctc
     6241 gtccgtttca tctaccgaca tctcgacaaa atcgaagccc aacgtttttg ccagttgcag
     6301 gcgttccagc cagcactccc cggcggggag cgctttttca tagatgccaa gcgggatttg
     6361 tttggacaac atatccgctc cttagcccca cagttcggcg atggaacgtt tgaactgacg
     6421 tgcggcttcc accggagaag cggcatcacg gatactacgg cccgcgataa agacgtgaat
     6481 cggaatgcct ttgaacagcg gcagatcttc cagcgccagg ccaccagtga cggtgacttt
     6541 gaagcccata tcggaaagac gtttgatcgc ggtgatgtcc gcttcgcccc acgccacgcc
     6601 tgcggcctgc gcgtcacggc tgcggtgata aaccacctgc tgaatacctg cgtcgcgcca
     6661 ctgttgcgcc tgttcccagg tccagtaacc ggtcagttcg atctgcacgt cgccgttaaa
     6721 ctcttttgcc acgtccagcg cgcctttggc ggtgttgata tccgcacagc aaattaccgt
     6781 cacccagtca gcgttggctt cgaagcacat acgcgaaagg attttgcctg catcggcaat
     6841 tttggcgtct gccagtacga ttttgtgcgg gtagagcgct ttcaggtcac gaaccgcacg
     6901 cacgccttcg cccacgcaca gaatggtgcc cacttcgata atgtcgactt cttcggcaat
     6961 caggcgagtg gtttcgtagg cgctatccat agtctggttg tccagcgcga cttgcaacat
     7021 cggtaatgac atgttcaatt ccttcttaag ctgccgcgtt ggtgcggtca atgagatcca
     7081 gtacttcctg ctcggtacgg caggcgcgta aacggtcaaa attctcttca tcttcaaaca
     7141 ggttgacgat ctgcatgatg cccacttcct ggtgagtatt ggcatcgacc gccgccatgg
     7201 tgatgaggat atccaccgga tcgttgtcat cgtggttgaa ctccagcggc tttttcagcg
     7261 ttaccagaga gaaaccggtt ttcttaacgc cctcttccgg acgcccgtgc ggcattgcca
     7321 ggcccggagc gataacgaaa taaggaccga actgttcaac gccatccaga atcgcctggt
     7381 agtaacgcgg ctctaccaca tctgccgcca ccagcaggtc aacgccgatt ttcaccgctt
     7441 cctgccatgt ctctgcttca gcctgcagac ggatggattt attttcagcc agcgaatcac
     7501 gtaatttcat ggcgcgtcct tacttcacgt cctgcgggaa atgctctttg atcacttcca
     7561 gcagtttcgg gccaaagtcg gcaggagaga gcatgttgcg cacgccaacc acgtatttgt
     7621 tgccggtcac ggtgatttcg cccgcaatgt gcgtagaagc gatgatgata tccgcgccac
     7681 tcaactcgct tttgtactcg ccaaccgcgc agctgtttac cgtatggtca atgtttgatt
     7741 gggttaaaaa ctggtccact ttcatcttca tgatcatgga actgccttgt ccgttgccac
     7801 acacagccag aatacgtacg gtcataatca aaactcctta ttaagcagac tgttctgcca
     7861 gttgtttttc tgcatcttct tctgcgcgca gcgcgcggcc agcgaagaac atataagcca
     7921 gtgcaattac aatgatgacg gccataaagg cgataccgat ggagaagaag ccttgcatca
     7981 tcggcggtgc cagaatcgac cagtccgcca tgcccatcca ggcactcata ccggtgagtt
     8041 tcaccgccca gacgcaacca aagatttcga tcattcccat caccagacaa atcttcagcg
     8101 ctgcacgcca gccgccgaag tggttagcga acacgccgat ggtggcgttg gagaagaaca
     8161 tcgggataaa gcctagaata atcagaatgg aagagccgca ggcgaccagg atgccaactg
     8221 caatcagctg accgatggtg ccccacataa agccccagac cacggcgttc ggcgcgaagc
     8281 tatagatagc cgcacagtca atcgccagaa ccgcacctgg gatcaggcgc tgggagatac
     8341 cgttaaatgc ttcagagagt tccgccacaa acatgcgcac gccctgagtg atgatgaaga
     8401 tcgccaccgc gaaggagaaa ccggtttgca ggatgtagac cgtccagtgc actttgcccg
     8461 ccatcgcctg cacggtgtcg ataccgaagg agagcagaat ggcgccaaag aagatggtca
     8521 tcacaatcgc cgtggagacg atgttgtcgt ggaaaatgtt cagccagccc ggcagtttga
     8581 ggtcttcaac gctctcctct tttttgccga ggaacggcgc gactttatag gcaatccatg
     8641 atgcaaactg ctgctggtga ccgatggaga aaccacagcc atccgtcact tcctgagtcg
     8701 gcttgtacat catgttggaa gtgatgcccc agtagagcga aaccagaatc gcggtacaga
     8761 taatggtggt ccacatggag tagccgaaga taaacagcgt aacggcaatc aacccggcct
     8821 gctggaacat gatgtggccg gtcaacatga ttgtgcgaat gccggtaatg cgacgcaaca
     8881 gcacgtaaca gatgttcagt gccagcgcta acaatacggc gtaacccacc cagctatagg
     8941 catcgcccat gcggtcgatg gttgccatca ttgaagcgta ggtatcggaa attgcgccgt
     9001 taatgccgta gacttcggac attttcgcca ccaccggttt gaaggtgctg gtgaggatgc
     9061 cggaccctgc ctgcaacaac atgaaaccaa ttatggtttt aatcgtgcct ttaataataa
     9121 cgctgacact tttgcgcagt aggatgtagc ccagacaggt cacaataccc agcaacaacg
     9181 gggcattggt catgacctgg ttaaaaaata cggtaaagat gttgtagagg atctccataa
     9241 cgatctcctg aagaagaggt aaccgggtat tccacacacc cggaatgttg tgcactcact
     9301 ctaattttca aaagtaatca caacaagatt atttatgatt aaatgtgacg cgacccgcaa
     9361 ataattccac atggatagag cgggattcac gcaacgaaac aattattcat ttaaattcat
     9421 ttagttaatt gaattataca ttccattaaa tctttccata ctcaaaatca acaacaactt
     9481 ccctggcaaa aagtcaaaaa atggtcattg ccttccagaa aaaatagtgt caggattaat
     9541 caaaaccaat cacatattga ttcgaattga actatcatga aggtaaatgg cgatgagtaa
     9601 agtgaaaagt atcacccgtg aatcctggat cctgagcact ttcccggagt ggggtagctg
     9661 gttgaatgaa gaaattgaac aagaacaggt cgctcctggc acatttgcga tgtggtggct
     9721 tggctgcacc gggatctggt tgaaatcgga aggtggcacc aacgtttgcg ttgatttctg
     9781 gtgcggcact ggcaaacaaa gtcacggtaa cccgttaatg aaacagggtc accagatgca
     9841 gcgcatggct ggcgtgaaaa aactgcagcc aaacctgcgt accaccccgt ttgttcttga
     9901 tccgtttgcg attcgccaga tcgacgcggt actggcgact cacgatcaca acgatcatat
     9961 cgacgttaac gtcgctgctg ccgtgatgca gaactgtgct gatgacgtac cgtttatcgg
    10021 accgaaaacc tgtgtggatt tgtggattgg ctggggcgta ccgaaagagc gttgcatcgt
    10081 ggtcaaaccg ggcgatgtag taaaagtgaa agacattgaa attcatgcgc ttgatgcttt
    10141 cgaccgtact gcactgatca ccctgcctgc cgatcaaaaa gcggctggcg tactgccaga
    10201 tggcatggac gatcgcgcgg tgaactacct gttcaaaacg cctggcggct ccctgtatca
    10261 cagcggcgac tcccactact ctaactatta tgcgaagcac ggtaacgaac atcagatcga
    10321 cgtggcgtta ggatcgtacg gcgaaaaccc gcgcggtatc accgacaaga tgaccagcgc
    10381 cgatatgctg cgtatgggtg aagcgctgaa tgcgaaagtg gttatcccgt tccaccacga
    10441 tatctggtca aacttccagg ccgatccgca agagatccgc gtgctgtggg agatgaaaaa
    10501 agatcgtctg aagtatggct tcaagccgtt tatctggcag gtgggcggca aatttacctg
    10561 gccgctggat aaagacaact tcgagtacca ctatccgcgc ggtttcgatg attgcttcac
    10621 tattgaaccg gatctgccgt tcaagtcatt cctgtaatct gtcgtttata ccggatagcg
    10681 tggagaagtt cgcgctatcc ggcgtagtat ttcctggcta tttcaaatat catctaaaaa
    10741 aatcaaattt tatcggaata gctcatgact gaagcacaaa gacatcaaat cctcctggaa
    10801 atgctcgcac aattgggctt tgtgaccgtt gagaaagtcg ttgagcgtct gggaatttcg
    10861 cctgccactg cgcgacgcga tatcaataaa cttgacgaaa gcggcaaact gaaaaaagtg
    10921 cgcaatggtg cagaagctat tacccaacag cgcccgcgct ggacgccgat gaatctgcat
    10981 caggcgcaga atcacgatga aaaagtacgt atcgctaaag cggcctcgca gctggttaat
    11041 ccgggcgaaa gcgtagtcat caactgcggc tccaccgcgt ttctgcttgg gcgggaaatg
    11101 tgtggcaagc cagtgcaaat catcactaat tatctaccgc tggcaaatta cctgatcgat
    11161 caagaacatg acagcgtgat cattatgggc ggacagtaca acaaaagtca gtccatcact
    11221 ttaagcccgc agggcagcga aaacagtctc tatgccgggc actggatgtt taccagcgga
    11281 aaagggctga ccgcagaagg gttgtataaa accgatatgc tgacagcaat ggcagagcag
    11341 aagatgctga gcgtggtagg gaaactggtg gtactggttg atagcagtaa gattggcgaa
    11401 cgcgcgggaa tgctttttag ccgtgccgat caaatcgata tgcttatcac cggcaaaaat
    11461 gctaacccgg aaatcctgca acaactggaa gcgcaagggg tcagcattct gcgtgtttaa
    11521 agatgctggc ggaaaaatgt cacggcagca tctaacgcct caggcgtaat gcggtgacgc
    11581 acgcctggct gccatgaaca ggttagcagt ttatcccgtc ccgtctcgct taaggcctgc
    11641 tgcaaacgta gtgattcgtc ggcaggcaca acgtcatcgt cgaggccata ccacagcagt
    11701 agaggtctgt cactaagttg ttccaggtgg tttgtcgctt cccactctgc cagtggcgcg
    11761 acaatgttat tgaattcatt ctgctgtgct accgtttcag gtatcagcgg tggaaacagt
    11821 gaacgggcga gtgatgtaaa atagcccgat cccatcatgc tggcggtaca tctcaccgtg
    11881 gggtggcgag cggtaatccc cagtgccgtc atcgcgccca tcgacgcacc accgactgcc
    11941 agacggtcat caagcagcca cttttcttcg gctattgccg cacgtaaagt agtgaattcc
    12001 tgcatacttt gtagcaagat ttgccagaat tgatttaacc gccgcgctgc gtcaccacta
    12061 aaacggctac cgtgatcggg cgcatccggc atgatcaccc gcaaaccagc ctgcgccagc
    12121 gcaacggcaa aatagctata caccagactg gacgaagtaa agccgtgata aaaaatcacg
    12181 cacggtaacg gggtatcttt ttgtccgaca ggataagcat gaagaacggg aatatctgcc
    12241 agctcgcgtg attctatttc aatcatctgt ctctcctttt tgtctggtat gacatgaaga
    12301 aatccttagc acaagtttct gctggcggta ttagttcata atgttgagat gtgggttacg
    12361 ctttcgtcac gttttcattc gaaaatcgcg taccaggtaa caattcggga acattccccc
    12421 aaagttaaat taataaggca ctacactatg gttagcagga aacgtaatag cgttatttac
    12481 cggtttgcca gtttattatt ggtgttgatg ttaagtgcct gtagcgcact gcaaggtacg
    12541 ccacagccag caccgccagt aacggaccat ccgcaagaga ttcgccgcga ccagacgcaa
    12601 gggttacagc gaataggtag cgtaagcaca atggttcggg gttctccgga tgacgcatta
    12661 gcagaaatca gagcaaaagc tgtcgctgca aaagctgatt attacgttgt cgtcatggtg
    12721 gacgaaacca tcgtgacagg acagtggtat tcacaagcca ttttgtaccg taaataaatc
    12781 agacaactta catagtcagc tttacattgc tttgcgtccc tatgcgttta cctgtgcaca
    12841 ataaattacc ggctggaagc ctgggcgttt attaccgcga tggaatgccc tgcaacatgt
    12901 ggggaaacga aaaatggagc tgacgatgaa acaattactt gcctcaccct cgctgcaatt
    12961 agtgacttat cctgcgagcg ccacggcaca gtctgcagaa ttcgccagtg ctgactgcgt
    13021 gacgggcctg aatgaaatag gtcagatctc agttagcaat atttcagggg atccgcagga
    13081 cgtggaacgt atcgtggcgt taaaggccga tgaacaaggt gcgtcatggt accgcattat
    13141 taccatgtac gaagaccagc agcctgataa ctggcgcgta caggcgatac tgtatgcatg
    13201 agtgtcatct acagcaaatg aacaatgccg gatgcggcgc gagcgccttg tccggcctac
    13261 aattttcaca tatttcattt agttaatcga aaccagcgtc gcatcagtcg tatacgctta
    13321 cacacacact ccccccgtcg cccgcagcaa taaatcattc tggatctgct ctgacaaccg
    13381 tacgccgccg cgcgtatcta acatcacctg acaccacgcc tgcgccattg gcggagaagc
    13441 atatttcaac atttgcgcac cgcagcccag caggaatagc tgatgagtaa tctctcgccc
    13501 cagttcttca gctggcttac gcagctgctg ctgtaaacga cgaaccgcgc aatcaaaata
    13561 gcgatcctgc cctttcactt ctacaaatgc ttccgacaat aagtcgtata cgcccgcttg
    13621 cttattgaga acgcgcagca catccaggca cataatattg ccggaacctt cccaaatact
    13681 gtttaccggc atctcccggt aaagccgcgg cagctcgctc tcctcgcaat aaccaatgcc
    13741 gcccagcacc tccatcgctt cggccacaaa cggcatacct cgtttgcaga tcacaaattt
    13801 tgccgcaggc gtaaataaac gcgcccacag agcttctttg gcatcggcgc gccggtccca
    13861 cgctcgcgca agacgaaaca gcaacgccgt ttgcccttca agctgaagtg ccatgcgact
    13921 taagacatga cgcataaggg gctgttggat caatggatta ccaaaaacat ggcgttgatg
    13981 cgcatgataa atcgccagcg agaacgcacg gcgcatcatg gcatggctac ccagggcgca
    14041 atcaaaacgc gtcatccccc ccattttcag gatcagacga attccttccc cttcctgccc
    14101 caataaccag ccaatggcat cctgaaactc cacttcacaa ctggcgttag agcgattacc
    14161 cagcttatct ttcagccgct cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg
    14221 cggcacaaaa aagcaggaca aaccgcccgc ggtctgcgcc agcaccagat gcgcatcgct
    14281 ttgcggaact gagaaaaacc atttatgccc caccagccga taagagccat cctccagacg
    14341 ctctgcgcgg gtggtgttgc tcatgacatc ggaaccgccc tgcttttccg tcattcccat
    14401 gccaatcaac aaaccgcgtt tttgcccacc tggcaataag tgagaatcgt agcgatcgct
    14461 caacagcggt gtggtccagt cctgaaacgg cgcgggtaac atctgtaaca acaatggcgt
    14521 ggcggcaaag gtcatggtta tcggacataa cgaccccgcc tcaacctgtg cgtgcaacat
    14581 aaaacgcgcc gcgcgcgcca caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt
    14641 gtgcacccga ttggtacata gtgcctgcat cagcaggtgc caggcggggt gaaaacgcac
    14701 atcgtccaga cggcgtcctt gcgcatcgta gcgcaacagt tcaggcggat tcacattcgc
    14761 cagccgcccc agttcaaggg attcagccgt tcctaactgc tgaccaatac tggctagaaa
    14821 atcgctatcc cagccagcac cttcacgcgt taccgcttcg caaagcgcgc catcagacag
    14881 gtataagttg ctgttattta atggtatagg ttgattaaaa acggtgtgag tttgccagtg
    14941 cactgtgtct ccctccatca atggcagtca ccattagtat ggtcactgcc atggattcat
    15001 gacagaaaga gattgctaaa acattcttat ccagcaatgt aatgcggaac gaaacgtgag
    15061 gtgtctttgg tgatcagtgt gttatcttca cggatcccca ttccgcacgc ttcatcatcg
    15121 acaatccagc taccgatgag tgtgtagcta tcgccaaacc gcggcagagg ttgaaacgcc
    15181 tggtagatca tcggttcatc ggcgtaatca ccatcagcgt ggtcaacgac gttattctgg
    15241 ccgtcgaaaa tggtgacatt tccgccttcg cgcgagtaga tcggtttacg cacatagctt
    15301 tcgccagcgg caatctgcgg tttttcaccc tcaaaccagg atgcaagaag gttaggatga
    15361 ccagggaaga agcgccaaag cagcggcatt agccctttat tactcaagat acttttccat
    15421 aacggctcca cccattgctc gcgacgcttg cgcagcagcg gaccgttatc gtcacgcatc
    15481 atccactcca gcggatacag cttaaatgca cgctggatga cattatcatc aagatcggtc
    15541 agtacgccgc cgacgcccaa accgagatct tcaatgtaga taaaccgcga ctcctgccct
    15601 gcctgctggg cgcagtcctg caagtacagc acggtactcc tgtcttcatc ggtgtcctga
    15661 cagcagcaaa aataaaacgg ttcccgactg taaagctcgc taaagcgtga aatcaggcgt
    15721 tcctgaatag cattgtactg atcggcatca cgcggaataa tgccgctgcg ccgggcatcc
    15781 tccagccaca gccactggaa ataagccgac tcgtacaatg aagttggcgt atcggcgttg
    15841 tactccagca gcttcaccgg cgcattgcca caccaggcaa aatccatccg tccatacagc
    15901 gaaggatcgc gggcgcgcca gctttcagcg atcacatccc agtacaacgg cgggatcgcc
    15961 agttgcgtca ggatctcttc atcttttacc gcgcgatcca ccacctcaag gcacatctga
    16021 tgcagttccg cagtcggttt ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa
    16081 gcccgactct catcccaata gatttcattg tcgatgatat gaaagtcgaa accgttgtca
    16141 gcggcgatct ggtccagatc ccgtcgcaca ggaacgttgt gtctcagcat gattagcctc
    16201 cccaatgccc acgggcgctg gaagaacgac catagccgcc gcgagaaacg gttgacgctt
    16261 ttttggtggt aaagccgcct gaagagtaag actctttttt gccagaaccg gagcgccagg
    16321 agtaatcacc ggaagtgttg cgccagacgg ggcgggaagc aaatgaggat ccgccgctgt
    16381 tatagacaaa cggctcatcg cgatctttac gaataacccg gcttaacaaa aatccagaaa
    16441 cgaccgggat ccaactctgt tcaacattgt cgtagtagca attttcgtac ttagactgac
    16501 agttctgctg agtcatattc ttcggaacat cggcataaaa tgccgctttg gcgttgttcc
    16561 agccacgcgc gcaaatgtcg gcattattac cgtcatcaat acaatcctgc actgtcgaat
    16621 aaaacgttcc gtcgccgtcg ttatcgacat cgccactatc gccacaacct ttcaatacaa
    16681 aaaaagcggc accgcccatt agcgccaacg ttaagtattt ttgtgcgtag cgattgcgac
    16741 gcggttcaaa aggattattc ggtctaccaa tgcgactgat cgctccgtga ccaattttac
    16801 tgttatttct tgatttgcgt tttctggcca tgatcgtcca ttaccatgtc atacaagctg
    16861 cgttaaaaat accgccagcc agcgcggcgg ttcccataaa cattcctgct gcggtattgt
    16921 gattaataat tttttcgctt aatgcgggca tataaagtct gacaccagca aaaacgagta
    16981 actgaatcac cagtgcaatc ccgccccagg cgaaatagtc tggaatactc accgcattga
    17041 ttgccgcact ggataagggg atcacgtaac ccaacaatgt accgctgaac gccagtgacg
    17101 ctgcggtatt attgtttttg attaattgcc attcattgtg cggtgtaatt ttggagtaga
    17161 taaacagaaa aataatcacc atagccacgc caataaaaaa ataggcacta aaggcaagaa
    17221 gtgaatccag tatatgcatt gtaaacgtcc ttgtaattaa ccaataatat gaagtgatgt
    17281 cagcgggata tcgacaccca acgcgcgcga aaataaccac tctggctcgc cgagatcgtt
    17341 aaaagattcc tcaccattta gcagcaaata ttcgtaagta tcttcggtca cttgccgctg
    17401 gtagcccatg gtaaaattat ggacttccca tttagcatga ttttgatttt ctactttttc
    17461 cagcatgtag acgggttcga tatttcccgg ttcttcgctg ttaaaaaatc gctgccagcg
    17521 tttttcctgc cagtttaagg tcattgcccc catcgttttg gcgttgatcg cctcgcgcca
    17581 gtgattctct ttactgatac cgtagctctc ttcatagaca aagagcttga tatcatcaat
    17641 gtcatcaata tcttcgccgc cggtggtatt gatttgcaga aactcatcgc ctgaagtgta
    17701 gtagcggaaa atctgactac cgccgcccag atcgatgtgg ctgacagcga ctaccgtaaa
    17761 ttcctcaccc ggcagcgcaa tcagcaattc atcttccagc aaacgaaacg ctaacgtgtc
    17821 gagcgtaaag ccactattga gatgaagtcc cagcggaccg cgagcgatag caggcttatt
    17881 atccttgcca aacagacgct ggaaaaaacc agacatattt tatccttaaa atagttgcca
    17941 gccttgcagt gaggctggca aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat
    18001 acatcctggg cgctactttt attgctaccg ccaattccgg cttccgccag cttttcgtca
    18061 aggtcgcgac cgtctgcgac tttttctaac tgtgcggcag catccaggcg agcctgacgt
    18121 tcggcctgac gcgtttgcag gcgttttaag gattctgctg ctgtcgaaac gctggaagat
    18181 gcgccaacgg tagaagtcgt tacagcctgt tgtgcacgct gcatggcttc agtggcttta
    18241 acgacttcca tttgctgctc aaactgagcg atacgctgcg ctgtcgctgc aactgctttt
    18301 tccacgccat cacgggagac ttccagattc gacaacactt gctcttcagc ggtaatgaga
    18361 ttctcaaggc gcgcgatttc ttcagcaact tcgttaatca acgacggatt aacgttcttg
    18421 cttaacgctt ccagcgtacg ggcttccaga ctggctttgc gctcacgtaa atctttcagc
    18481 ttatcgtgac ttaatttcac ccgcgccagc agatcaacgc gagattttcc ggctttatcg
    18541 agttcagctt tagcgtcacg aatatgctgt tccagcatgc gcacaccctg ggtttcttca
    18601 atggattctt ccgcctggga gataaacgat ttccccagcg taaataaact ttttaaaatt
    18661 cccataaata cccgtccttg taattagtgt gaatattctt ctgtaatttc agccagatcc
    18721 aatgcgttat ctaccagcga ggttatctca agcaggatat cttcaagaga agatttaagc
    18781 gataacgccc cgaaaacaat gtaatactct tcttgttgta cgctggagat tcctaccgat
    18841 gacaacggca tcatcttctg atttcttaat aagaaggtat taaattcatc tggattgctg
    18901 atactactca ccggacaaat aaaggtttcg ataatcattt ggcgggaagt aaaaagaata
    18961 ttgatttgca aatcgccata atcattcatt ttaataatta atgcgttctc gctatttgtt
    19021 acatcaatat tttgttcagg tacagtttgc agcgccgtcg ccagcgcc
//
LOCUS       DAZFKY010000084        18762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFKY010000084 DAZFKY010000000
VERSION     DAZFKY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            186..491
                     /locus_tag="ACHKCL_002630"
     CDS             186..491
                     /locus_tag="ACHKCL_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763581.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            598..1242
                     /gene="gfcB"
                     /locus_tag="ACHKCL_002631"
     CDS             598..1242
                     /gene="gfcB"
                     /locus_tag="ACHKCL_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5763582.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1239..1985
                     /locus_tag="ACHKCL_002632"
     CDS             1239..1985
                     /locus_tag="ACHKCL_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5763583.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1985..4081
                     /locus_tag="ACHKCL_002633"
     CDS             1985..4081
                     /locus_tag="ACHKCL_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5763584.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4127..5266
                     /locus_tag="ACHKCL_002634"
     CDS             4127..5266
                     /locus_tag="ACHKCL_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5763585.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5254..5700
                     /gene="etp"
                     /locus_tag="ACHKCL_002635"
                     /gene_synonym="yccY"
     CDS             5254..5700
                     /gene="etp"
                     /locus_tag="ACHKCL_002635"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5763586.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5720..7900
                     /gene="etk"
                     /locus_tag="ACHKCL_002636"
     CDS             5720..7900
                     /gene="etk"
                     /locus_tag="ACHKCL_002636"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5763587.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8020..9324)
                     /gene="appA"
                     /locus_tag="ACHKCL_002637"
     CDS             complement(8020..9324)
                     /gene="appA"
                     /locus_tag="ACHKCL_002637"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5763588.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9404..9496)
                     /gene="cbdX"
                     /locus_tag="ACHKCL_002638"
     CDS             complement(9404..9496)
                     /gene="cbdX"
                     /locus_tag="ACHKCL_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5763589.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9509..10645)
                     /gene="appB"
                     /locus_tag="ACHKCL_002639"
     CDS             complement(9509..10645)
                     /gene="appB"
                     /locus_tag="ACHKCL_002639"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5763590.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10657..12201)
                     /gene="appC"
                     /locus_tag="ACHKCL_002640"
     CDS             complement(10657..12201)
                     /gene="appC"
                     /locus_tag="ACHKCL_002640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO5763591.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12335..13192)
                     /locus_tag="ACHKCL_002641"
     CDS             complement(12335..13192)
                     /locus_tag="ACHKCL_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5763592.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13189..13587)
                     /gene="hyaE"
                     /locus_tag="ACHKCL_002642"
     CDS             complement(13189..13587)
                     /gene="hyaE"
                     /locus_tag="ACHKCL_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5763593.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13584..14171)
                     /gene="hyaD"
                     /locus_tag="ACHKCL_002643"
     CDS             complement(13584..14171)
                     /gene="hyaD"
                     /locus_tag="ACHKCL_002643"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5763594.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14168..14875)
                     /gene="hyaC"
                     /locus_tag="ACHKCL_002644"
     CDS             complement(14168..14875)
                     /gene="hyaC"
                     /locus_tag="ACHKCL_002644"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5763595.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14894..16687)
                     /gene="hyaB"
                     /locus_tag="ACHKCL_002645"
     CDS             complement(14894..16687)
                     /gene="hyaB"
                     /locus_tag="ACHKCL_002645"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5763596.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16684..17802)
                     /gene="hyaA"
                     /locus_tag="ACHKCL_002646"
     CDS             complement(16684..17802)
                     /gene="hyaA"
                     /locus_tag="ACHKCL_002646"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5763597.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18228..18315
                     /locus_tag="ACHKCL_002647"
     tRNA            18228..18315
                     /locus_tag="ACHKCL_002647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18262..18264,aa:Ser,seq:tga)
BASE COUNT         4589 a         4995 c         4847 g         4331 t
ORIGIN      
        1 acaggtcgta tttccagagg ggaatagtta acaatcttat atttccctct gcgcgccttt
       61 ctcacccgct gtatatagtg caagtggtat gaggaaaggg agatttcaca aggatgttat
      121 actcaaagta gacaatcctg atctcaagcg tacgtattgt ccatgcgatg aataaaggaa
      181 aagttatgaa acacaagctt tctgcaatcc ttatggcctt catgttgacg acgccagctg
      241 cgtttgctgc ccctgaagca gcaaacggga ccgaggcaac gacgggtact actggcacca
      301 caacgaccac taccggtgca accacgacgg ctgctaccac tggtggcgtg gctgctggcg
      361 ctgtcggcac ggcgacagtt gtcggtgtgg caacggctgt tggtgtggcg acgttggcag
      421 tagttgcagc aaatgacagt ggcgatggcg gttctcataa cacctcaacc accacgtcca
      481 ctacgcgcta attctgctta aacgcgtcga aattaaagtt aaataatacc cggttgcgat
      541 attcgtaccg ggctttagag atttgcattt ttttaattaa cctttcaagg attaaaggtg
      601 cgccctctta ttttatcgat tttcgcacta tttcttgcgg gatgtacgca cagccagcaa
      661 agtatggtcg atacatttcg cgccagcctt ttcgataatc aggatatcac cgtagcggat
      721 cagcagatcc aggcgttgcc ttattccacg atgtatttac gccttaatga agggcaacga
      781 atctttgtgg tactgggata tatagaacaa gaacaaagca aatggttatc ccaggataac
      841 gccatgctgg ttacccacaa tggacgtctt ttaaagaccg tcaaacttaa taataatctg
      901 ctggaagtga caaattccgg gcaggaccct ctgcgtaacg cgctggcaat aaaagacggc
      961 agccgctgga cgcgcgatat tctctggagt gaagacaacc attttcgctc tgcgaccctg
     1021 agttccactt tttcctttgc tggattagag acgctgcata ttgcgggtcg cgatgtgctg
     1081 tgtaatgtct ggcaggaaga ggtgacttcc acgctaccag aaaaacagtg gcaaaacaca
     1141 ttctgggtcg attcggctac tggccaggtt cgtcaaagtc ggcaaatgtt aggcgcaggg
     1201 gttattcccg tagaaatgac gtttcttaaa cccgcaccat gaataaatta cagtcgtatt
     1261 tcattgccag cgtactttac gtaatgacac cccatgcctt tgcgcaagga acggtgacta
     1321 tttatctgcc tggcgaacaa cagacacttt ccgttggccc cgtggagaat gttgcccagt
     1381 tagtgacaca accgcaactg cgcgatcgcc tctggtggcc tggtgccctg ctgaccgact
     1441 ccgcggtgaa agctaaagcg cttaaagact accaacatgt catggcacag cttgcttcct
     1501 gggaagcaga agctgacgac gatgtggcgg caacgattaa atccgtgcgc cagcagttac
     1561 tcaatctcaa tattactggt cgcttaccgg tcaaactgga ccctgatttt gtacgagtgg
     1621 atgagaacag caatcccccg ctggtcggga actatacgct ctataccgta cagcgcccgg
     1681 tgactattac tcttctgggt gcggtttctg gggctgggca attgccctgg caggcaggcc
     1741 gtagcgttac cgattatctg caagatcacc cccgtcttgc aggcgcggac aagaataacg
     1801 ttatcgtgat tacgcctgag ggtgagactg tcgtcgcccc tgttgccctg tggaataaaa
     1861 gacatgtgga accaccgcct ggcagccagc tttggttagg cttttctgcg catgtactgc
     1921 ccgaaaaata tgctgattta aatgaccaga ttgtgtctgt tttaacgcaa cgtgttcctg
     1981 actgatgaag aagaattctt atcttttaag ctgcctggcc attgccgtct ccagtgcctg
     2041 tcatgctgaa gtattaacct acccggatcc gctgggttcg tcgcaatcgg actttggcgg
     2101 cacaggattg ttgcagatgc caaatgcgcg catcgcaccg gaaggtgaat tcagcgtcaa
     2161 ctaccgggat aacgatcaat accgtttcta ctccacctcc gtggcgctgt tcccatggct
     2221 ggaaggcacc attcgttata cggatgtgcg cacacgtaaa tatagccagt gggaagattt
     2281 cagcggcgat cagtcataca aagacaaatc attcgatttt aaacttcgcc tgtgggaaga
     2341 aggttactgg ctaccgcaag tggcgtttgg taaacgtgat attgctggta cgggtctgtt
     2401 tgacggtgag tatctggtgg ccagcaagca agcggggcca tttgatttca ccctcgggat
     2461 ggcatggggc tacgccggta atgcgggcaa tattaccaac ccgttttgcc gggtgagcga
     2521 taaatattgt catcgcgcag agtctcacga tgcgggcgat atcagcttta gcgatatctt
     2581 tcgtggcccg gcttccatct ttggcggcat tgagtatcaa acgccgtgga atcccctgcg
     2641 tctgaaactc gaatacgacg gcaacaatta ccagaatgat ttcgctggca aactgcctca
     2701 ggcaagccat ttcaacgtcg gtgcagttta tcgcgctgcc agctgggcag acctcaacct
     2761 gagttatgaa cgcggtaaca cgttgatgtt tggtttcact ttacggacca atttcaacga
     2821 tctgcgccct gccctgcgcg atacgccaaa accggcatat caacctgcgc ctgaatctga
     2881 aggattgcag tacaccacgg tagcaaacca acttaccgcc ctgaagtata acgcgggctt
     2941 tgacgcgcca gaaattcagc tacgcgataa gacgctgtat atgtctggtc agcaatacaa
     3001 ataccgtgac tctcgtgaag cggtcgatcg tgccaaccgg attctggtga ataacctgcc
     3061 gcaaggcgtt gagaagatta gcgtgacgca aaagcgcgag catatggcga tggtgactac
     3121 cgaaaccgac gtagccagcc tgcgcaaaca gctggcaggt acagcgcctg gtcaatcaga
     3181 gccactgcaa caacaacgtg ttgaagctga agatctttct gcctttggtc ggggctaccg
     3241 tattcgtgaa gatcgcttta gctactcttt caacccaaca ctttcacagt cgctgggcgg
     3301 cccggaagat ttctatatgt tccagctggg gctgatgtcc agcgcccgct actggtttac
     3361 cgaccacctg ctgcttgatg gcggtatttt caccaatatt tacaacaact acgacaagtt
     3421 taagtcttcg ctgttgcccg cggactctac cctgccccgc gtgcgcacgc atatccgtga
     3481 ttacgttcgc aatgacgttt atctcaacaa cttgcaagcg aattactttg ccgacttagg
     3541 caatggtttc tatggccagg tgtatggcgg ttatctggaa acgatgtatg ccggtgtcgg
     3601 ttccgagctg ctttatcgcc cgctagatgc cagctgggcg ctgggtgtgg acgttaacta
     3661 cgtgaagcag cgtgactggg acaacatgat gcgcttcacc gattattcca cgccaactgg
     3721 tttcgtgacg gcttactgga acccgccgac gctcaatggc gtactgatga aacttagcgt
     3781 tgggcaatat ctggcaaaag ataaaggggc aacgatcgac gtcgccaaac gctttgacag
     3841 cggcgtggcg gtaggggtat gggcggcaat cagtaacgta tctaaagatg actacggcga
     3901 aggcggcttt agtaaaggtt tttatatctc gattccgttc gacttgatga ccattggacc
     3961 taaccgcaac cgcgcggtgg tttcgtggac accattgacg cgtgatggtg gacaaatgct
     4021 gtcacgcaaa taccagctct atccaatgac ggcagagcga gaagtaccgg ttggacaata
     4081 acctgacgta attcataaca ttacattgac tgataaatat aaaccaatga aaaagaacat
     4141 ttttaagttt tcggtactga cattggctgt tctgtctttg acagcatgta cccttgttcc
     4201 tggtcagaat ttatcgacca gtaacaagga tgtcattgaa ctacctgaca atcagtatga
     4261 cctggacaaa atggtcaata tctatcccgt cacgccagga ttgattgatc agttgcgcgc
     4321 caaaccgatt atgtcgcagg ctaacccgga gctggagcag caaatcgcga actatgaata
     4381 tcgtatcggc attggtgatg tgttgatggt tacggtctgg gatcacccgg aactaaccac
     4441 ccccgcaggg caataccgta gcgccagtga taccggcaac tgggttaacg ccgatggtgc
     4501 gattttctac ccctatattg gtcgcctgaa agtggccggg aaaacactta cccaggtacg
     4561 caacgagatt acagcgcgtc tggattcagt cattgaaagc ccgcaagtcg atgtcagcgt
     4621 cgcggcgttc cgctcgcaaa aagcgtacgt caccggtgaa gtcagcaaat cgggtcagca
     4681 gccgattacc aatattcctc tgaccatcat ggatgcgatt aacgccgcgg gcggtctgac
     4741 cgcagatgca gactggcgca atgtggtgct cacccagaat ggcgtaaaga ccaaagtgaa
     4801 cctctatgcg ctgatgcagc gtggcgactt gcgccagaac aaactgctgc acccgggaga
     4861 tattttattt attccgcgca acgatgatct gaaagtcttc gtgatgggtg aagtgggtaa
     4921 acagagcacg ctgaaaatgg accgcagcgg tatgaccctg gcggaagcgc tgggtaacgc
     4981 tgagggtatg aatcaggatg tcgctgatgc gacggggatt tttgttattc gtgcaacgca
     5041 aaacaagcag aatggcaaaa tcgccaatat ttatcagctg aatgcgaagg acgcatcggc
     5101 aatgatactc ggaacggaat tccagcttga accttacgac attgtttatg tcactaccgc
     5161 gcctctggct cgttggaacc gtgtgatttc cctgctggta ccgacgattt ccggggtgca
     5221 tgacctgaca gagacttcac ggtggattca aacatggccc aactaaaatt taactcaatc
     5281 ctggtggttt gtaccggcaa catttgccgt tcgcccattg gcgagcgctt gctgcgtaaa
     5341 cgtctgccgg gcgtaaaagt caaatcggca ggtgttcatg gtctggtaaa acaccctgca
     5401 gatgcgacag cggcagatgt tgccgctaat catggcgtat ctcttgaagg acatgccgga
     5461 cgtaagctca ctgctgagat ggcacgaaat tacgatctga ttctggcaat ggagtcggaa
     5521 catattgctc aggttaccgc aatcgctccc gaggtccgcg ggaagacaat gctgtttggg
     5581 caatggctgg aacagaaaga gatcccggat ccctatcgta aaagtcagga tgcatttgaa
     5641 catgtctacg gaatgttgga gcgcgccagt caggaatggg cgaagcgcct cagccggtaa
     5701 tttgagttta taaaatacga tgacaactaa aaatatgaat acgccaccag gcagcactca
     5761 ggaaaatgag atcgatctgc ttcgtctggt cggcgagtta tgggatcacc gtaagtttat
     5821 tatcagcgtg accgcgttat tcacgctgat cgctgtcgct tactcgctgt taagcacacc
     5881 aatttatcag gcagatactc tggtccaggt tgagcaaaaa cagggcaacg ccattctcag
     5941 cggcctgagt gatatgatcc ctaactcatc gcccgagtct gcaccggaga tccaactgct
     6001 gcaatcgcgc atgattctcg gtaaaaccat tgctgaactg aatctgcgcg acatggttga
     6061 gcagaagtat tttccgattg tgggtcgcgg ctgggcgaga ttaaccaaag agaaaccagg
     6121 tgagctggcg atcagctgga tgcatattcc acaactgaat ggtcaggatc agcaactgac
     6181 actcacggtt ggggaaaacg gccactatac gctggaaggt gaagagttca ccgtcaatgg
     6241 tatggtcggc cagcgtctgg aaaaagatgg cgttgcgctg actatcgcgg atattaaggc
     6301 caaaccagga acacagtttg tcctgagcca acgtaccgaa ctggaagcga ttaacgcgtt
     6361 gcaggaaacc tttaccgtta gcgaacgcag taaagaaagc gggatgctgg aacttaccat
     6421 gactggtgat gatccccagt tgattactcg tattctgaac agcatcgcta acaactattt
     6481 gcaacagaat atcgctcgcc aggcggcgca ggattcacaa agccttgaat tcttacagcg
     6541 ccagttgcca gaagtgcgca gcgagctgga ccaggcggaa gaaaaactca acgtttatcg
     6601 ccagcagcgc gattcggttg accttaacct ggaagccaaa gccgttcttg agcagattgt
     6661 gaacgttgat aatcaactca atgagctgac cttccgcgag gcagagatct cccagctgta
     6721 taagaaagat cacccaactt atcgtgcgct gctggaaaaa cgccagacgc tggagcaaga
     6781 acgcaaacgc ctgaataagc gggtatcggc tatgccttcc acccaacagg aagtgttgcg
     6841 tttaagtcgt gacgtagaag cgggccgtgc ggtatatctg caattactta accgccagca
     6901 ggagttgagt atttcgaaat ccagtgccat tggtaacgtg cggattatcg acccggcagt
     6961 cactcagccg cagccagtga aaccgaaaaa agcgttgaat gtggtgcttg gttttattct
     7021 tggcctgttt atttcggtgg gtgccgtgct ggcgcgtgcg atgttgcgtc gtggtgtaga
     7081 agccccggaa caactggaag agcacggcat cagcgtttat gccaccatcc cgatgtccga
     7141 gtggctggat aaacgtaccc gtctgcgtaa gaaaaattta ttttctaatc agcagcgcca
     7201 tcgtactaaa aatatcccct tcctggcggt ggataacccg gcggattctg ctgtggaagc
     7261 cgtacgtgcg ctacgaacca gtctgcattt cgctatgatg gagactgaga ataacattct
     7321 gatgatcacc ggtgcgacgc cagacagtgg taaaacgttt gtcagttcaa ctctggcagc
     7381 ggtgatcgcc cagtccgatc aaaaagtgtt atttattgat gccgacttac gccgtggtta
     7441 ttcgcataac ctgtttaccg tgagtaatga acatggcttg tcggaatatc tggcaggtaa
     7501 agatgagctc aacaaagtga tccagcattt tggcaaagga ggctttgatg tgattactcg
     7561 cggtcaggtg ccacctaacc catctgaact gctgatgcgc gatcggatgc gtcaattact
     7621 ggaatgggcg aacgaccatt acgatctggt gattgttgat acgccgccga tgctggcggt
     7681 gagcgatgct gcggtcgtgg ggcgctctgt cggcaccagc ctgctggttg cgcgttttgg
     7741 cttgaacacc gccaaagagg tgagcttgtc aatgcagcgt ctggaacagg ctggcgttaa
     7801 tattaaaggc gcaatcctca atggtgtgat taaacgcgcc agcaccgctt acagttacgg
     7861 ctataactat tacggttata gttactccga gaaagagtaa aactgaaggt aaagtgaccg
     7921 gatgcggcgt gaacgccttt tccggtctac gcattagaca gttcttcgtt tgtcatcagc
     7981 ctcagagcat tcaggtagct gaatgctctt ttttatacat tacaaactgc acgccggtat
     8041 gcgtgcttca ttcacgattt gcgtaaaacc ggccaacgaa cacatgccct gcgcatttcg
     8101 ctcttcacat cctgccaggg tcagtttcac ctctccgggc ggcgtattta atgacagcgg
     8161 agttttatca cgcatctgct gtaaagtctg gaagaccagc gaaacctgaa tccactggct
     8221 gttatcgctt agccgacgcc agcgttcaaa caccagttca ccacctggcg gcgtgttatc
     8281 cggctgacct ggaagcgtcc agttgagctc cagtgcgccg ccgagatttg ccagattagt
     8341 atcgtgtccg gcgataaaca gcactgaagt gggtaatgtc acaccatacg cctgtttttg
     8401 cggtggatgg ggagtcaacg ctatcatgat caaatccaac aacggggtgg cgcggctgcg
     8461 ggcaacctct ggcgtgcgtt gtagtaaata aaattgcgcg ttatgcaaac ttagcaacgt
     8521 gttccactgg tgtgaatcag tgatccttcc ccaccccggc tccggcattc cctgtgcctg
     8581 ttgcaggaga aatatttccg tcagcattga tgcgaggctt accgcaccgg ttaatgagac
     8641 attgtcggcg ctcaccttga gttccgatgg taatgcctgc gttaatgaac agctttcgtc
     8701 ctgtttctca cggttaaggc acaagtttga ttgcggaaaa ttaagcaccc gttccagttc
     8761 gcgaaacgcc gtttgccgat gcccggtaaa gtcagcaatt gaccctcctg ccctgctgag
     8821 gatcgcgtca gtcacgttcg cgttatccag ttggcaaacg ccagttttta gaggattaaa
     8881 taacggatcg ggactggacg tatctgcctg ggtatgtacg gttattgcac agtcaggtgc
     8941 cagcccggcg gcgaaggctt cgcctgtttt acgggtacgc tcgtcgacat cagcaataat
     9001 cgcgacctga ccaggctgcg ggcagccctt tttcgtcagc aatccgtcgg ctaccagacg
     9061 ctggcgttgg taatgtccga gataagcgat tagctcacca ccgcgcggtg tcagccaacc
     9121 cagttttacc ggccagtttg gccatgcgtc tggggtgaca tcctgcatca gttgcgtggc
     9181 cttggttggg gcacgcacac catgacgact gacaatcacc acactttcca gcttcagctc
     9241 cggctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9301 aaatgggatc aagatcgctt tcatcgatat gtttccgctt ttcatatccg acattattga
     9361 ttgcctgata cgacgctaat gcgccgcatc agacttacgt tcgttaactt ttcaatcgcg
     9421 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
     9481 aaagtaaata ccacattgtt cctgctcctt agtacagctc gttttcgtta cggcggagag
     9541 tttctgtcgt catgcgcccc cacattttgt agtagctcca gagagtgtag agcaacacaa
     9601 tgggcaaaaa tatcagcacg attaccaaca taatactcag cgtcagctga ctggaagtgc
     9661 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
     9721 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatc aaaaatcccc
     9781 aacccgggcg gccacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
     9841 gcgggaagat ccacaaaacg ggcgactcga caaaattatt catccaggca ccaggtagca
     9901 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
     9961 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10021 gtgcagcgcg tttagtcgac agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10081 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10141 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggagtga
    10201 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10261 ccagactgcc gatgaccaga ccggcatccc acattttacg ccagcgtgca tcggcgattt
    10321 ttccgcgata atcaaaggcc agcgggcgga agaacagcga gcacagcacc aggatcatcg
    10381 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10441 cacctgcgag gatcaaccag acctgattgc cttcccagtg cgcaccaacg ctattaatca
    10501 ctatccggcg ttcatcatca tcacgcgcca ccagcggcag caaacagcca atccccatgt
    10561 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    10621 agcgcaatgt ttcataatca aacatgattt tctcctttac ccctgttgct gcgtcggttg
    10681 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    10741 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    10801 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    10861 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    10921 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    10981 cgcaatcagc atcaccagta gcagcaggga accacagccc accatgatgc ggaaactcca
    11041 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11101 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcgccttc
    11161 cagctcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11221 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattctt
    11281 cagacccggc acgggtttat ctaatgagtg agtggcgagg atccctaaca gcacgggaat
    11341 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11401 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    11461 ttgcgcgact tcatacgcag aactgtctcc gagttgcagg gtaccgataa tcgccagagt
    11521 accgaagacg gaaccgatgg caaacgagcg taatgcgaca tcgcgctccc gtccgcgcag
    11581 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    11641 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    11701 ctccatacgc agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    11761 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    11821 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    11881 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    11941 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12001 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12061 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12121 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12181 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgtta
    12241 atggtagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12301 attcataagc ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12361 actgacatac ctcactaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12421 ccacttccgg tattgggcag atttcataac tttccagtaa cgggcctttt aaggtgtccg
    12481 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    12541 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    12601 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    12661 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    12721 gtaacagcga atctgtcggc aagagatttt gcgttgccgc ctgccacaac gccagcggcg
    12781 cgcagccagc ctccagtttg tcctccagca acttttcgcc gcgacgtctg cgcacccgcc
    12841 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    12901 gcactgacac ttcaccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    12961 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13021 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13081 ccatactcgg ttcatcattc acctgacagg tgatcggcag tggattcatg ctgaaactgt
    13141 catcgttcgg ttgcgttctt ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13201 ctcctgctgt ggttcgacaa gcccacgcat caggtttatc agttccgccc acgggtgaat
    13261 accattcagc acgccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13321 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13381 tgtgtagtcg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13441 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    13501 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    13561 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    13621 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    13681 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    13741 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    13801 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    13861 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    13921 atacgttctc cggcataggt tcgcagcgtt cctggttcca gcccgtagtc aatggcatcg
    13981 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14041 ccatcgacaa tctccacaga ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14101 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac caccacgcgt
    14161 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14221 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14281 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14341 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14401 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    14461 tcaggaaata gccaaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    14521 gacgttttgc cagaaacaga taccagcgga tttcatacca cacgccctgc caccagcttt
    14581 tacgccatac tggcacgata aacagctcgc gggagtatcg attgccaaca aaagcccagt
    14641 agatccgcat cagcaagacc acggtaaaaa ccatcccggc gctgaagtga attaacctga
    14701 tgtagcccat atagaacaga tacgtcgcct cgccgctgac ggaaggtagc ggtttgccga
    14761 taaagtaacc ggtgaccatc aacaccgcca tgcataacac cgtcagccag tgccagatac
    14821 gcactggcgc ttcaaagaca tagtggctga caacgttgtc gcttttctgt tgcatgatga
    14881 ttctccttcg ctgttaacgc acctgcacgg agatcagctc gctaccgtcg tcgcccagca
    14941 cgtgtgttga acaggcgagg cacggatcaa agctgtgcag agtacgcagg atctccagcg
    15001 gttgctcggg gatcgccatt ttggtgttca tcagcgccgc ttcataagcg ccaatctgcc
    15061 ctttgggatc gcgcgggctg gcgttccagg tggtcggcac cacgcactgg tagagatcaa
    15121 tcttgccatc gcgaatggcg gcccagtggc ctaacgcccc gcgcggcgct tcggtaaaac
    15181 cgacaccacg gcactctgtc ggccaggttg tgggttccca tttttccgtg gaggcggtgg
    15241 caagattgcc gtttttcagg ttagtcatca gcttgttgaa gaaatactgc aacttacctg
    15301 cggcccactg tgcttcgtgc gcgcggcaca aaatgcggcc caacgttgac tggataccgg
    15361 aaagcggcag gttcagcgcc gacatcatgc gatcgaccga ctcaacggtc gcagcatcgc
    15421 ctttgtgata agcgattaac gtgcgtgcca gcggccccac ttccatcgcg ttaccgcgcc
    15481 agcgcggcgc tttgatccac gagtagcgtt cctgttcatt cagctgctga atgttggtat
    15541 cgctgccttt gacatcgccg gggttgtacc acgggtcggt gatgccatcg aacggatgac
    15601 gcccgacctg atcgttggga taacgatacc aggcgtgatc gacaaactcc tgcacctgct
    15661 gcggatcaac caaatccact ggcagcacat tgttgaagtc gccgttaatc accgcgccgc
    15721 caggcatcag cagacttttc tcaccaaagt cgttggcaat atccgggaat gcgccgtagc
    15781 tgaggacaca tttatcagaa agacccgtgc cgatttcgct ccacggtttg ttgaactgac
    15841 cgatggctaa ggcgtcgggg atcatcacgt tgttaatgaa gtctgccgtg cgggtgataa
    15901 tcgactgcac caggttcagg cgttccatat tgaccgcccc gaccgcgccg ctttcgtcaa
    15961 tgttaatggc gcaaggcatc ccgccgacaa tccagtttgg atgcgggttt ttaccgccaa
    16021 agaccgcgtg gattttgaca atttcacgct ggaaatcgag agcttcgaga tagtgggcaa
    16081 agcccatcag gttggcttct ggcggcaact tgtactgcgg gtgcccccag tagccattgc
    16141 ggaagatccc caactgcccg ccttcaacga atttcttcag gcggttttgt acgtcgaaga
    16201 aatagccagg ggatgatttc ggccaagagg agagactttg cgccagttcg gaggttttcc
    16261 gcgggtcggc tttcagcgca tccaacacat cgatccagtc catcccggca agctgataga
    16321 agtgcaccag atgatcgtgg caccagagcg ttgccagcat aatgttgcgg atgatattgg
    16381 cgttgtccgg cactttaata ccgatggcat cttcgatggc gtaaaccgaa gccagagcgt
    16441 gtacgccagt acagacgccg cagatacgtt caacgaacgc ccacgcatcg cgcgggtcgc
    16501 gcccttgcag gatgatctcc agcccgcgaa acatggtgcc gcaggagacg gcattggtga
    16561 ttacattctg atcgttaata ttcacttcgc agcgcatgtg gccttcaatg cgcgtaatcg
    16621 ggtcgaccac caggcggcgt ccggcattat tgatggtgta tccctgagtt tcgtactgag
    16681 tgctcatgcc tgtttatcct cattgcctgg ctgatgttcg gtttctgtag gttgctgatt
    16741 atgacgtctg cgctggtcaa cggcgctggc gactgcgtgc acaccaacag ccgctgccac
    16801 cacaccaagc gcggttaaac cgacggtatc ggcggtggaa tgagtaccca tttgtggaat
    16861 atcgaccacg cggctgtaga acgaaccgcg atcccagaaa ccattttccg cacagcccag
    16921 gcagccgtga ccagactgga ttgggaaaga aacgccatca ttccagcgtg tggaggaaca
    16981 ggcgttatag gtggtaggcc ctttgcagcc cattttgtac aggcaataac ctttgcgggc
    17041 agcgtcatca tcccaactct ggacgaactc tccggcgtcg aagtgagcgc ggcgatagca
    17101 tttatcgtgg atacgctgac cataaaacat cagtggacga cccattctgt cgacatctgg
    17161 caagcgatca aaggtcacca tgtaagtaat gatggcgctc atcacatccg ggatcggcgg
    17221 gcagccaggt actttgataa tgggtttgtc ggtgatgact ttgtcgatag acgttgcctg
    17281 tgtcggatta ggtcgcgcgg cctgcacgca gccccaggac gcacaggttc cccaggcgat
    17341 aatcgcgctg gctccggcag cggcacgttt gagtttctca ataaacggtc gaccgctgct
    17401 gatacagaac atcccctgct cgcccagcgg cggattacct tctactgcga ggatatattt
    17461 gccattgtat tgcgtgatga tgtcttcaaa gacttcttcc gcctgggttc cggcggcagc
    17521 catcaaagta tcgtcgtaat cgagggaaat cagggaaagg atgacgtcct ttgccagcgg
    17581 gtgagcggag cggataaaag attcggtaca gcaggtgcat tccagaccgt ggatccatac
    17641 caccggaatg cgcggtttgt tctccagcgc ccaggcaatc tttggtgcca ttcccgcgcc
    17701 taatcccagc gacgtggcag ccagactaca atatttgaga aagctgcgcc gggtaacgcc
    17761 ctgacgccgc atggcctggt aaaatgtttc ctcgttattc atatcgcacg tctcttctcc
    17821 ttgcgacacc ggcaggacac cgggcgctta tggtcggcaa gcgacgtccc tgtagcaagg
    17881 caaaccatcg cttcacgtga gatgctgaaa acgaaagctc atccttctgc actggcgcac
    17941 gtcgccagaa agtattgtta ataaagcgta gtgaaacttt tgcacaaaac aatacaaact
    18001 gtgtggattt atcttttagc gataaaaatg gacctatttt tctttttgcc gggcggtggg
    18061 atgtttagcc agttgctaaa cgagtaaaag agaaggaatt tatggtgatg aaatgatcaa
    18121 tctgctttta tttcacgcta tcaatgcact tttggctgtt tttcaggcaa acaaacagat
    18181 taggggttta cacgccgcat cgggatgttt atagtgcgcg tcattccgga agtgtggccg
    18241 agcggttgaa ggcaccggtc ttgaaaaccg gcgacccgaa agggttccag agttcgaatc
    18301 tctgcgcttc cgccaaataa tcaaggggtt accaaatggt agcccctttg cttttttggc
    18361 tcttggtata aacttggtat accaattggt atatttttat tcggatgaga ttatcggtca
    18421 tgagcggaac ctcaggttcc tcgtctgatg ccgctcttgc aacacgttac gctgccgagt
    18481 atttttgtaa aacatggact gctccaggcc tgaaccaggc agaaggatac aaagccatct
    18541 cagatctgtc gcatcactat tttcgtgcag aaggctctag ccctccccaa tcttttttgt
    18601 gacaccctgt ttcactgaac atgaagacac tcaccttgtc cctgatattc attagcatca
    18661 tcctgattat ttaacttctc tatttaggga agcccgtctg atacaaagct tctattcctc
    18721 gttaaatatt ctccattctg gcgccaagcc ctatctatag ct
//
LOCUS       DAZFKY010000085        18664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFKY010000085 DAZFKY010000000
VERSION     DAZFKY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(2..580)
                     /gene="mocA"
                     /locus_tag="ACHKCL_002648"
     CDS             complement(2..580)
                     /gene="mocA"
                     /locus_tag="ACHKCL_002648"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5763598.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            683..1453
                     /gene="yqeC"
                     /locus_tag="ACHKCL_002649"
     CDS             683..1453
                     /gene="yqeC"
                     /locus_tag="ACHKCL_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5763599.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1501..3126
                     /gene="yqeB"
                     /locus_tag="ACHKCL_002650"
     CDS             1501..3126
                     /gene="yqeB"
                     /locus_tag="ACHKCL_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5763600.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3167..4099)
                     /gene="arcC"
                     /locus_tag="ACHKCL_002651"
     CDS             complement(3167..4099)
                     /gene="arcC"
                     /locus_tag="ACHKCL_002651"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5763601.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4147..5532)
                     /gene="hyuA"
                     /locus_tag="ACHKCL_002652"
     CDS             complement(4147..5532)
                     /gene="hyuA"
                     /locus_tag="ACHKCL_002652"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5763602.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5585..6796)
                     /gene="ygeY"
                     /locus_tag="ACHKCL_002653"
     CDS             complement(5585..6796)
                     /gene="ygeY"
                     /locus_tag="ACHKCL_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5763603.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6854..8050)
                     /gene="dpaL"
                     /locus_tag="ACHKCL_002654"
     CDS             complement(6854..8050)
                     /gene="dpaL"
                     /locus_tag="ACHKCL_002654"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5763604.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8108..9295)
                     /gene="ygeW"
                     /locus_tag="ACHKCL_002655"
     CDS             complement(8108..9295)
                     /gene="ygeW"
                     /locus_tag="ACHKCL_002655"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5763605.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9774..11552
                     /gene="ygeV"
                     /locus_tag="ACHKCL_002656"
     CDS             9774..11552
                     /gene="ygeV"
                     /locus_tag="ACHKCL_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5763606.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11592..12071)
                     /gene="xdhC"
                     /locus_tag="ACHKCL_002657"
     CDS             complement(11592..12071)
                     /gene="xdhC"
                     /locus_tag="ACHKCL_002657"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5763607.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12068..12946)
                     /gene="xdhB"
                     /locus_tag="ACHKCL_002658"
     CDS             complement(12068..12946)
                     /gene="xdhB"
                     /locus_tag="ACHKCL_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5763608.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12957..15254)
                     /gene="xdhA"
                     /locus_tag="ACHKCL_002659"
     CDS             complement(12957..15254)
                     /gene="xdhA"
                     /locus_tag="ACHKCL_002659"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5763609.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKTFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15670..16425
                     /gene="actS"
                     /locus_tag="ACHKCL_002660"
     CDS             15670..16425
                     /gene="actS"
                     /locus_tag="ACHKCL_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5763610.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16504..16577
                     /locus_tag="ACHKCL_002661"
     tRNA            16504..16577
                     /locus_tag="ACHKCL_002661"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16536..16538,aa:Gly,seq:ccc)
     gene            16811..18416
                     /locus_tag="ACHKCL_002662"
                     /pseudo
     CDS             16811..18416
                     /locus_tag="ACHKCL_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            18483..18662
                     /locus_tag="ACHKCL_002663"
     CDS             18483..18662
                     /locus_tag="ACHKCL_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO5763611.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT         4673 a         4407 c         4726 g         4858 t
ORIGIN      
        1 tttaaatttt agtatacctt ttttgcgctg taataaagtc gtccggagta tcaatatcta
       61 aaattatttc tgcattttct acttcgacgg aataatgagc tcccataagg agtgcctgac
      121 gcatattggt gatattgggg cgcttgattg cctgcatcag gcatggtttt gacactaaaa
      181 tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt cgtaacgacc
      241 agatttttct gaaaatatcg atggtgaggg ttggcatgtc accgtgggtg agaaaacaat
      301 gttctgtttg cactgcgggt actgcggcct ttactgatgt tagtaaaccc tgcgcataat
      361 ctgggttgtg gataatagtg atattgctct ggttcgcata acgttcgtgc agttcattac
      421 cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt ttgatacttg
      481 tatcaagaat tgtcccctgt tgccagggta acatcatttt ccattgcccc attcttgatg
      541 ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca ttatttatag
      601 gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg gataatttat
      661 cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat tattcattga
      721 tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg ggaaaaccag
      781 cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt taatcacgac
      841 gacaacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc gtgatcccgc
      901 catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca gctggaaagc
      961 gaacctggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg tgcaacgacc
     1021 agagtgtgac gtaattctca ttgaagcaga tggctcgcgt gcaatgccgt taaaagcgcc
     1081 tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg tgatgggagg
     1141 acatattttg ggcgcgaaag tgagcacgga aaatgtccat cgctggtcgc agtttgctga
     1201 tattaccggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg cgctggttcg
     1261 ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct ggttcattaa
     1321 ccgtttttct caatgtgaga atgcgattgc gcaaagcgag ctccttcaac cgctgcaaca
     1381 acacgacgta gaggcaatct ggctgggcga tatacaagaa catcctgcaa tcgcgcgcag
     1441 atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat tgaggttggt
     1501 atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt tgctatggcg
     1561 caaattattg atagccgagg ctcgactccc cgccattctg cacaaatgtt agtgcgcgcc
     1621 gatggctcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt gattgaagag
     1681 tcgcttcagg cattgcagga acgtaagccg cgattattcc atggacgtat ggctcgtaac
     1741 ggtgcggatg ctgtcgggtc agattgtgga ggtgcgatgt cagtgtttat cagcgtccat
     1801 ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc gatagcccag
     1861 agtgcggcgc tattaggatt tgatattgcc gttgccgata tttatcgcga aagcctcaat
     1921 cctgaactat tccctccatc aaccacgctt ctccatgctg agtcgtttgg tgcggcagtg
     1981 gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa tcaggatcgt
     2041 gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct ggcaagtcgt
     2101 cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga agaacatatt
     2161 gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca ggagatcgcc
     2221 atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg gctgatgatg
     2281 aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg agatatcgcc
     2341 agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct ggaagtggaa
     2401 aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga tggcgaaatg
     2461 acggtcgaag gcgtcactgc tcgcctggca accagctctg cggaagcgat gaaacttacc
     2521 gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga tgaactgaaa
     2581 ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacacg ggcagatatg
     2641 gcaccagtaa caatcgcgct tgggccgggc tttgctgcag ggaaggattg tcatgcggta
     2701 attgaaacaa atcgcgggca ctggctcggt caggtgattt actgtggttg tgcgcaggag
     2761 aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat ccgtgctcct
     2821 gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga gggcgatgtg
     2881 attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt gcgtggcttg
     2941 ttgaacgacg gcctggcagt ggttggtggt tttaaaattg gtgatatcga tcctcgtggt
     3001 gaaaccgctg atttcaccag cgtttctgat aaagcccggg cgattggcgg cggcgtactt
     3061 gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt gctggaagtg
     3121 gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac tgacaaatca
     3181 ccgttccggt tttgccttca atcccttctt tcgctttgct caatacggta atcagtgctt
     3241 cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc agcatagaac
     3301 ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac aggcgatcga
     3361 gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca gtcagaatga
     3421 tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct ttatcgataa
     3481 ccgcgctggc accacgcaga tggttacctt cacgaataac aggaatacca ccaccgccaa
     3541 cggtgatcac cacctggcct gcttctacca gcgcttttac cgtctctttt tcaatgatat
     3601 caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct tctttcagag
     3661 tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa ccgatcggct
     3721 tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta accagcgtta
     3781 ctacaggttt attaatgccc cgagaaagca gttcttctct taaggcgttt tgtagatcgt
     3841 aaccaatata accctggctt aaggcaacac agacagacat cggcagcatt ggcgaatgcg
     3901 cttcagtttt tgcggcagct tcaaaggcct ggttaatcat gcccacctgc ggcccgttac
     3961 catgagtgac gataacttca tgtccctggg caattaaatc aacaattgcc tgagaagtaa
     4021 tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg cccagggcga
     4081 gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat agatccctgg
     4141 cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac ctcgcccggc
     4201 tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg ttctgacaat
     4261 cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt ggagatggcg
     4321 atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat cggaaccggg
     4381 cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg cgctggttaa
     4441 ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa acagtaactg
     4501 catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc ctttagaaat
     4561 ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt caatcgcacc
     4621 atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg gtgggctaag
     4681 aatgaacttc atgccgtctt ctgtatcgta actgcgttcg tccaacagga gatattgtgg
     4741 gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat aatccagacc
     4801 taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt gtgccaggtt
     4861 aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg catgatagcg
     4921 cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag ccgcatcatt
     4981 ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga cggcgtaatg cctgcaaaac
     5041 ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc tcagcccttc
     5101 ctcgaccatc atcgggattt cgtcgaggat ggcgtgatta atgtgctgga tcacaccgtg
     5161 aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct ccagttgatg
     5221 gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg ttgtaccgcc
     5281 acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga tgccgacatc
     5341 aatattgaaa tgcgtatgga catccacgcc gccagggaaa acgtaacagc cagtggcatc
     5401 aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa caatcccgct
     5461 ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc cgtttttgat
     5521 caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa taagtgaaga
     5581 gtaattattc ggttgccagc cagcttaacg ggattgcagc gtacatcgcg gcacaggtca
     5641 ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct ttacccgggc
     5701 caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta gttgagaagg
     5761 tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc accagtgctt
     5821 taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg gttgggtaaa
     5881 ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg ttagctttct
     5941 gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg ccccaggtca
     6001 gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt ggggatgtga
     6061 agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa cccagacgtt
     6121 gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg ttgtcaccgc
     6181 gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt tccatacgac
     6241 cgcgctgacc acggtatacc tggcagtcgg ttggttcggt actgaccaca aattccgggc
     6301 gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct tcttcctgca
     6361 cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta atgattttac
     6421 cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga ccaccgatca
     6481 gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg ttgccaatgc
     6541 cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg atataaccga
     6601 gaacgttacc catcgggtcg atttcaactt tatcgaagcc gactttttcc atctcttctt
     6661 taatacgatg tactacccgt ttctcgtcgc agctttcact gggaatagca accatgtcgc
     6721 gcaggaagcg agtcatatcc gcctggtaat cttttgcttt ttcaagaatc agtttgaatg
     6781 gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc agggagcagt
     6841 tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact tcgcggtagt
     6901 gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac tacggcatct ttgttcagcg
     6961 ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg agaacgccca
     7021 aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac ggattaccca
     7081 gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg gtggcgcagt
     7141 tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggcct gccatgatgg
     7201 tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga taaatacagt
     7261 cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctatag acgtcgacca
     7321 gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga acgtgcgtcg
     7381 gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt gcgtagcctt
     7441 gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt accacttccc
     7501 agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag ttcatatctg
     7561 tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga gcagaacctt
     7621 tcggcatgta aatcaccgca ttctgtccga gttgctgcgc tgcccacgcc acaccgcgcc
     7681 cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca tttttcaggt
     7741 gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat tgagcgatag
     7801 cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa tcgttttgat tcgtctttga
     7861 caagaatttt cttcacccca aaaaggtttg cgagatcgtc cagcgcacaa agcggtgttg
     7921 ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg ctttgcgaaa
     7981 agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc ttcaatgaga
     8041 aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag ctgatcggcc
     8101 cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc agtgcacctg
     8161 gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc cagctggctt
     8221 ctttgtaggt agcgatacgg tatttttcga atacgccttc agtcacttcc ccttctttgc
     8281 aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg ccatcacggg
     8341 tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc tgtgccagac
     8401 actgtttttc cagtgctttt aagccttcgt gatcgttagc gcgcagcaat tcggtacgct
     8461 gttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgca tctttgaacg
     8521 cttcttccat gctggtgacc tgacggaagc taccgccaga tgctttagca ttgtttttcg
     8581 cgacttctac tacatccggg atcaggtcgt agccttccgg atgggccagg gtgacatcca
     8641 taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt ttgccatagc
     8701 ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc agtgaaccaa
     8761 agtgttcacg taaccatgcc aggtcagcca ttgactgagt cgggtggtca atatcgcatt
     8821 gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg tcatcaagtg
     8881 cagcgccaac ttcacgcata taggcgttgc ctgcgcccag atacatatcg tcgcgaatac
     8941 caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt tcgccgtgag
     9001 cgatttgtga tttgccttca tcgagatctt gctgtgcaag gccgagcagg ttaagcgcgg
     9061 aagcataaga gaagcgggta cgagtggagt tgtcgcggaa tacggaaata cctaatccac
     9121 tattaaagac tttggttgag atgttttcag cacgcagtgc ttttaatgct gcggcaacgt
     9181 ccagtacttg tttcagttca tctggagtct gttcccacgt taacaaaaaa tctttctcgt
     9241 gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc attaacagtt ttcatcataa
     9301 agtcccggta gtggaatgac aggcaaagtg gtttatttca ctgtgacttg ccaaagtgat
     9361 tggtgttttc acagctatac ctgggactaa caataagtgt gccagaatta tccacggggc
     9421 tgcaaaagac taatttgacg gataatgtgt ggggttgatc acaaattgat gccagcttcc
     9481 ggttgtttgt aaaatacgaa aacgtttggc tttttttatc gcgttattta tttgctgcta
     9541 agtatcagaa ataagataaa gggacaggct atccctcgtc agtttgataa actcaccgcc
     9601 ataattctca tattgatata gaaatgtgat atctcttcca taatttcatt agaaagtgat
     9661 aaaaatctta tgtttttatc aaagccaatt tcgcctgagt taatatgatc ttgatctgtg
     9721 aaccatcaac gtcttcaatg atcgactttt aagtaagtct ggaaggtaaa gctatggagc
     9781 ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac aattcagcgt tttgccagaa
     9841 tgcttgccag cgttttgcag cttgaggttg agatcgttga tgaaaacttg tatcgcgtcg
     9901 ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt gagcggcaac tcacgcctgc
     9961 tctgccacgt cctggaaacg aaaactgaaa aagttgtgac acagtctcgc ttcgatcccc
    10021 tttgcgaagg ttgcgatagt aaagaaaatt gccgcgaaaa agcatttctg ggtacgcctg
    10081 tcattttaca ggatcgttgt gttggggtga taagtttgat tgccgttacc cacgagcaac
    10141 aagagcatat cagtgataat ttacgcgaat tttctgatta tgttcgccat atatccacca
    10201 tttttgtttc gaaacttctg gaggatcagg ggccaggaga taacatcagt aaaatattcg
    10261 cgaccatgat cgataatatg gatcagggcg tattagttgt tgatgatgaa agtcgggttc
    10321 agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt gcaaaataat attattggga
    10381 aacctatccg tttcagacca ttaacatttg agagtaattt tacccatgga catatgcagc
    10441 atattgtttc gtgggacgat aaaagtgaat taatcattgg tcaattgcat aacattcagg
    10501 gccgacaatt atttttaatg gcgtttcacc aatcgcatac cagtttttct gtagcaaatg
    10561 cacctgatga accgcatatt gaacaattgg ttggcgagtg ccgtgttatg cggcaattaa
    10621 aacgactcat tagccgtatt gcacccagcc catccagcgt tatggtggtt ggtgaaagcg
    10681 gcacgggtaa agaagttgtc gcccgtgcaa tccataagtt gagcggaaga cggaataaac
    10741 cctttattgc tatcaactgt gccgcgattc cggagcagct tctggagagc gaactgttcg
    10801 gttatgttaa aggcgcattt actggcgctt ctgccaacgg taaaacaggg ttgattcagg
    10861 cggcgaatac gggcacgctg tttctcgatg aaataggtga tatgccatta atgttgcagg
    10921 ctaaattact gcgcgctatt gaggcacgtg aaattctgcc gattggtgcc agtagcccaa
    10981 tacaagtcga cattcgcatc atttctgcaa ctaaccagaa tttggcccag ttcattgccg
    11041 aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt tatcccgata actctgccac
    11101 cgctgcgtga acgtcaggaa gatattgaac tactggtgca ttacttttta catctgcata
    11161 cccgtcgtct gggatcggtt tatcctggca ttgctcccga tgtcgtcgaa atattgcgta
    11221 agcatcgttg gcccggaaac ctgcgcgagt taagcaattt gatggaatat ctggttaacg
    11281 ttgttccttc aggtgaagtt atcgacagca cgctattgcc gccaaatctg ctgaataatg
    11341 gcacaacgga gcaaagtgat gtaacagagg ttactgaggc acacctgtca ctcgatgatg
    11401 cgggcggcac ggcgctggag gagatggaaa agcaaatgat ccgcgaggcg ctttcacgtc
    11461 ataacagcaa gaagcaagtt gctgatgaac tgggcatcgg cattgctacg ctctatcgca
    11521 agattaagaa atatgagttg ttaaacacat aacatcagtg aatccggcct gaattcaggc
    11581 cggatatcct tttacttcgt tttctcgcaa tccagaactg tatttacaat catctgatac
    11641 cccgtgcagc gacaaagatt tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc
    11701 ttctcgcgtg gtttcgccag cattgccgtg gtagccataa tcaggccagg cgtacaaaac
    11761 ccgcactgca ctgcgccgga tttcgcataa gcctgctgaa catgagaaag ttttccgcct
    11821 ttcgcttcac cttccagcgt gcggatctct tttccttcag cccaggcggc aaggtataag
    11881 caactgtcta ttgctgtgcc gtcgaccagc accgtacagg caccacattc acccacgcag
    11941 cacccttgtt tgacacttag cagcccttgt tcgcggagta attccgagag cggcgtgcct
    12001 ggtacggcat gaagctgaaa aggcatcccg ttaatggtgc attcgatggt aattgtttcg
    12061 ctgtgattca ttgcaatttt ccccccgccg cggcgacggc ttcgctaatc acttttttgg
    12121 tcatcgtctg gatgagatgc agacgaaact ctttactggc ccgccatgaa gaacgcgggg
    12181 cgacatcttg caggacagat tcgctgatag cttccagcgt ttgcaggttt aatggcgcat
    12241 tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt tggcgcggca acaccaaatg
    12301 ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca atgtgcggca cagccaatcg
    12361 ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg cgcgctgccc acgtgttctt
    12421 tcggctgggg cggaaaatga aaggcgacaa ggatttcgtc atgctcaaga gacactttgc
    12481 ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac accgcgtggg gagtggatct
    12541 ccagtttcgc gtcataaatg agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc
    12601 aaatatttcc accgtaggta gcgacgttac ggatctgcgg tccagcaatg gacgaggccg
    12661 cagcacataa cgccgggaga tgacgttgag ttatagaatc ttctattagc tgggtaaatg
    12721 tcgttgcaga gccgatacgt agcgagccat cttccgccag cgtaattccc cgcagctccg
    12781 ccagattatg aatatcaaca atatggcgat aacggtcatt gtgatggtgg agctgaatca
    12841 gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt gtcagccagc aggttgatgg
    12901 catcggcaag ggttgctgcg cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa
    12961 atcaatcctg ccagatggaa ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg
    13021 attgcaacac cggtagccat cttcaccgcg ttacgaatag cagcggcaac aggaattatt
    13081 ggtggctcac ccagtgactt atgtccgtat gcggattgcg gctcattgat ttcgacgaac
    13141 gcgctttcca gttgtggcag atccggcatg gtcggcattt tgtaatccag cagattgggg
    13201 ttacggacca cgccgctttt cgcatcgatg atcatctctt caaatagcgc ccagccaatg
    13261 cccattccca ttccgccgtg tacctgacct tctgccagta gcggattaag aatatggccc
    13321 gaatcatgaa cgttgaggat gcggttgatg gtgactttgc acagcgcaat atcgaccgtc
    13381 agatcaacaa aggtacagcc gaacgccggt gggttagtgg tggttttgat ggagctttca
    13441 gcagagagct gcccgccgcg ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac
    13501 gacattaacg gctcttccgg tcgttcaacc agcacgatat ggccttttat cagggtcaga
    13561 ttcatcgctg actgatgtag catgactgcg gcgtgagcga tgattttctc ttttaataac
    13621 agtgccgcac tgcgcagcgc aggcgcggca acatagctct ggcgtgaggc aaatgcgccg
    13681 ggatcgaacg gcgtaacgtc ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga
    13741 acccccacgg tttctgccac catttgcgag aagacggtgt cggcaccctg accgatttcc
    13801 gtcgcgccgc tttgcacgtt gatggttcca tcctgattca tcagaaggcg cgcgcctgct
    13861 atttctacgc cgacaggcca ggtgttagag gtgtagctaa aacaggcgac gccaacgccg
    13921 cggcgtaaat tgccttgctg gttctggcac tctgcacggc gtttttccca ttcaaagatt
    13981 ttccggcctt tttcaagaca ctccggcaac cctgcgctgt aaatacgttt gcccgtgagc
    14041 ggattagcat ctccttcgcg tgaggcgttg cgtaaacgaa tttcaacagg atcaatacct
    14101 aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa atacgacttg tggcgcgcca
    14161 taaccacgca tcgcaccagc cgaggggagg ttggtatagc aggtctttga actgtaagcg
    14221 taggcacaac gaggataaag gtaagcgact ttatttcccc ctgcagaagc gatggagtgc
    14281 ccgtgagata cataagcgcc ggtgttagac agaacatcca gactataacc tttcaatgtt
    14341 ccgtcgcggt tcacgcccat ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg
    14401 aggaaacact cttcacggct aagggaaact ttcaccggaa tgccgccaag cttgctggtc
    14461 aggaatgccg ccattggctc ttccagtaca tcctgtttat taccaaaacc gccaccgaca
    14521 aatgttttga tgactcgtac gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg
    14581 cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc
    14641 cacgccagcg atgttacgct ttccatgtga caatgttgaa taacgggggt ctgatagtgc
    14701 ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg
    14761 ctttgtttca gtaaattgcc gccgttatgg attggtgcag cgtcctctgc cagcgccgct
    14821 tctggcgtgg tgataacggg taattcttcc cactcaatgc tgaccaattg cgccgctttt
    14881 tctgccgtga gttcatcgcg ggccacgacg atggcaacgg cgtcaccatg atgacgaaca
    14941 tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt tttcgtcaag tgtccatgca
    15001 tgcccagctg tagcgaatgg gatatcaggc acatcttccc aggtaaaaat cgccagtacg
    15061 cctggtaaac ttctggcttg ttcatcatta atacttacgg cataaccatg tgcgataggg
    15121 ctacgtacat atttcgcata acacatgccc gccataacat aatcgtcagt atatcgtgcc
    15181 cgcccggtga ccttagcaat ggcatcaacg cgcatgcatg attcacccgt agcggttgct
    15241 tcccgcgctt ccatagaaat cccctcgatt tgaacatttg gttcttatgg caaagattta
    15301 gtctaagcag gcaagattta cgccagaaac ggcgataagc gttaagggag gaaggaagtg
    15361 tgatgatgtt aaaaaaacac agatatgata cgaaacagga ccctgaatag gggtagtgat
    15421 acgtatcaat atgagtggcg ataaaatcaa tgtgataaat taggattatt acgcatcagg
    15481 atgggaaata gggtaaaaaa gtcaggaact tcgatacctc tacagtgaat attttgctga
    15541 tgatacttcc ctttgccaaa tgaaattaac ctctttctct attacatcgt ttttccagaa
    15601 gcaagagatt gggtatcaag aggctggctg ctatgatatg gcgtcttgtt ttttaagcga
    15661 ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc tgggtatcgt gatgttgtta
    15721 tcggttggac tgcttttggc gggctgttcg ggtagcaaat catccgatac aggaacgtat
    15781 tccggctccg tttacaccgt gaaacggggg gatacgctat atcgtatttc gcgcaccacg
    15841 ggaaccagcg taaaagagct ggcgcgactg aacggcattt ccccccctta caccattgaa
    15901 gttggtcaga aactaaaact gggtggggcg aaaagtagca gtagtacacg taaatcaacc
    15961 gccaaatcaa cgaccaaaac cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc
    16021 tggccgccag tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg
    16081 tattcgacag cagatggcgg caataaaggg attgatatct cagctccacg gggtacacct
    16141 atttacgccg cgggtgcagg aaaggtggtg tatgtgggca accagctgcg tggctacggt
    16201 aatctcatca tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg
    16261 atgctggtaa ataatgggca aagcgtgaag gctgggcaaa aaatcgccac catggggagc
    16321 acggatgcgg catctgttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat
    16381 ccgctacgtt acttgccgcc tcagggcagc aagccaaaat gctgatggcg aattaatcag
    16441 cagtcagcag cgcggcactt gctaagaaga gcgtaaggtt tataatgcct tacgcatctc
    16501 gaagcgggcg tagttcaatg gtagaacgag agcttcccaa gctctatacg agggttcgat
    16561 tcccttcgcc cgctccaatt tatcttcacc tcatcaatct gccatttttg ctgtatctga
    16621 ttttctatat tatcttaatt caatcgcttc agacttttcg caatctctgt aacactcccc
    16681 catttttttg taaataaaaa gcgtttacgc aatgcaatcc ttttgtaaca acccaggtaa
    16741 aataccttat tttcaagttg ttaaataagt ggttatctac gcttttaaca aacgattgat
    16801 ggaatatttt atgaaaggga aatctgcatt aactcttctg ctcgctggta tattttcgtg
    16861 cggcacatgc caggcgacag gggcagaagt gacgagtgaa tctgtattta acattcttaa
    16921 ttccacgggg gcagcaacag ataaaagtta tttatcgtta aatccagata agtatcctaa
    16981 ttatcgtctt ctgattcact cagccaaatt acaaaatgaa ataaagagcc actataccaa
    17041 agacgaaatt caaggattgc taacattaac agaaaacaca cgaaagttaa cgttgacgga
    17101 gaaaccctgg ggaacgttta ttcttgcttc tactttcgaa gatgacaaaa cagcggcaga
    17161 aacccactat gatgctgtct ggctgaggga tagtttatgg ggctatatgg cattagtatc
    17221 agaccaggga aatagtgtag ccgcaaaaaa ggttctgctc accctgtggg attatatgtc
    17281 tacgcccgac cagattaagc ggatgcagga catcattagc aacccaaaac gactggatgg
    17341 tattcccggg caaatgaatg ctgtgcatat ttgacagcaa ctcccccgtg atggcagatg
    17401 tgcaggaaga aggcaagccg cagctgtgga accataaaca aaatgatgcg ttaggtcttt
    17461 atcttgatct ccttatacaa gcaatcaata ctggtactat taacgctgaa gattggcaaa
    17521 aaggtgaccg actaaaatcg gttgcgctgc tgattgctta tctggataaa gccaatttct
    17581 atgttatgga agattccgga gcgtgggagg aagatgcccg attaaacaca tcttccgttg
    17641 cactggtgac ctctggactg gaacgtttgt caaacctact ctcgaagaaa gactctgttt
    17701 ttgtctctga tttactacgc gaagctaaag tcaatgaact ggatgaaact ctttcaacaa
    17761 cgaggttgaa ccacctcata gataaaggtt atgaaagaat aacgttacag cttgatttag
    17821 ggggagaatc cccaggttat ctggaaaaag ataaacatta cagagaagcc gatgcggctt
    17881 tacttaatgt tatttatcct gcaaatcttt caaagataaa caccagaaga aaagagcaag
    17941 ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac gagaaggata
    18001 attatcagtc ggcaaatttt tggttcaatg atattaaaac cgataccgat caaaattcgc
    18061 atgcaaaaag agaaaagagc ttcattccat caacagaagc agaatggttt tttgactcat
    18121 ggtatgcaaa atcagcagca attgtttata aagaatcaag aaaagaagag tatttgaatg
    18181 actcagtaca atttatgaat agatcattgg cccaaataac aggcgagaat atgattggtg
    18241 caaatggcag gagcgtacca gaaatggcac ttcccgaaag ctataactat atccacaagt
    18301 cgggaacttt gcatgaagcg ccaagcccta tcattccgct aaattggtcg aaagcttcaa
    18361 tgactctcat gttgaaagag atgtccaacc ttatcaatga tgagggaatt aaataatttt
    18421 cataatgagg cggtcctgga ttaaaaggac cgctcaaaga gaaatcgaaa ggggaggtaa
    18481 aagtgatcaa taaattactg ttagcttacc tgataggtct ggttgtgaca tcgactttaa
    18541 tatttatctt ctcggaagaa aaagtaacat atcggttgtt tgctgcaata attacaggtt
    18601 taacctggcc gcttagtctc attccttcca ttataagtct catgattcgt aaatccgact
    18661 aaca
//
LOCUS       DAZFKY010000086        18624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFKY010000086 DAZFKY010000000
VERSION     DAZFKY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(21..644)
                     /gene="rsmG"
                     /locus_tag="ACHKCL_002664"
     CDS             complement(21..644)
                     /gene="rsmG"
                     /locus_tag="ACHKCL_002664"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5763612.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(708..2597)
                     /gene="mnmG"
                     /locus_tag="ACHKCL_002665"
     CDS             complement(708..2597)
                     /gene="mnmG"
                     /locus_tag="ACHKCL_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5763613.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2976..3419)
                     /gene="mioC"
                     /locus_tag="ACHKCL_002666"
     CDS             complement(2976..3419)
                     /gene="mioC"
                     /locus_tag="ACHKCL_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5763614.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3509..3967)
                     /gene="asnC"
                     /locus_tag="ACHKCL_002667"
     CDS             complement(3509..3967)
                     /gene="asnC"
                     /locus_tag="ACHKCL_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5763615.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4119..5111
                     /gene="asnA"
                     /locus_tag="ACHKCL_002668"
     CDS             4119..5111
                     /gene="asnA"
                     /locus_tag="ACHKCL_002668"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5763616.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5116..6567)
                     /gene="viaA"
                     /locus_tag="ACHKCL_002669"
     CDS             complement(5116..6567)
                     /gene="viaA"
                     /locus_tag="ACHKCL_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5763617.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6561..8057)
                     /gene="ravA"
                     /locus_tag="ACHKCL_002670"
     CDS             complement(6561..8057)
                     /gene="ravA"
                     /locus_tag="ACHKCL_002670"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5763618.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8280..10148
                     /gene="kup"
                     /locus_tag="ACHKCL_002671"
     CDS             8280..10148
                     /gene="kup"
                     /locus_tag="ACHKCL_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5763619.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10315..10734
                     /gene="rbsD"
                     /locus_tag="ACHKCL_002672"
     CDS             10315..10734
                     /gene="rbsD"
                     /locus_tag="ACHKCL_002672"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5763620.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10742..12247
                     /gene="rbsA"
                     /locus_tag="ACHKCL_002673"
     CDS             10742..12247
                     /gene="rbsA"
                     /locus_tag="ACHKCL_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5763621.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12252..13217
                     /gene="rbsC"
                     /locus_tag="ACHKCL_002674"
     CDS             12252..13217
                     /gene="rbsC"
                     /locus_tag="ACHKCL_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5763622.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13242..14132
                     /gene="rbsB"
                     /locus_tag="ACHKCL_002675"
     CDS             13242..14132
                     /gene="rbsB"
                     /locus_tag="ACHKCL_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5763623.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14258..15187
                     /gene="rbsK"
                     /locus_tag="ACHKCL_002676"
     CDS             14258..15187
                     /gene="rbsK"
                     /locus_tag="ACHKCL_002676"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5763624.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15191..16183
                     /gene="rbsR"
                     /locus_tag="ACHKCL_002677"
     CDS             15191..16183
                     /gene="rbsR"
                     /locus_tag="ACHKCL_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5763625.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16149..17576)
                     /gene="mdtD"
                     /locus_tag="ACHKCL_002678"
     CDS             complement(16149..17576)
                     /gene="mdtD"
                     /locus_tag="ACHKCL_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5763626.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17599..18291)
                     /gene="yieP"
                     /locus_tag="ACHKCL_002679"
     CDS             complement(17599..18291)
                     /gene="yieP"
                     /locus_tag="ACHKCL_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5763627.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4491 a         4614 c         4866 g         4653 t
ORIGIN      
        1 tatgattttt ttgataaaaa ttaaatttta tttgctttaa tcaccaccag atgacgttcg
       61 ccatccaggg ctggaacctg aagtttaacc actgattcga cctgatattc ttcgggcaac
      121 aaagcgattt catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca
      181 ggaagatggt ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg
      241 ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtactgg ctcaatattc
      301 tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc
      361 aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgccagg cagtccagga
      421 ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg
      481 agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg
      541 ttccatttat gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc
      601 gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct
      661 tgatgcgttg cctggtaagc gggggcttac caggcatttt taatgcgtta tgcgctacga
      721 cgcagcatac cctgtttttt cagccacacc agcagaatgg agatggccgc aggcgtgacg
      781 ccagaaatac gtgaagcctg gccgatagag gccggtttgt gatcgttaag tttggcgatc
      841 acttcgttag aaagaccgga tacctggcgg taatccagtg tcgcgggtag caaggtgttc
      901 tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta
      961 acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc
     1021 agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct
     1081 tcacgggaaa gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg
     1141 gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta
     1201 aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc
     1261 agatccgcat tatcttcgcg cagcatcaga cgatattctg cgcgcgaggt aaacatacga
     1321 tacggttctt tggttcctaa agtgcacagg tcatcaacca gtacgccgag ataggcctga
     1381 gaacgcgccg gagcccaacc ttctttgtca tcagacagac gggcagcgtt aagaccggcc
     1441 agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag
     1501 aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgagg atcgaagaag
     1561 tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatagag
     1621 cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt
     1681 tcgttagaag tcagcccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag
     1741 cgcatgactt tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct
     1801 gcgtacattg ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta
     1861 tgggtgatat aacacggcac ctgctgtgga tgctgggacg cattgcccat aaaagagaat
     1921 accggcattg ggttatcgcc atgctgttgc gcaagcacgc taaagtcgat ggttcgagca
     1981 tcaatacgcg gtggtgtccc ggttttcaga cgaccaacgc gcagcggaag ttcacgcaaa
     2041 cggcgagaaa gcggaatgga cggcggatca ccagcacggc caccgctgta gttatccaga
     2101 ccgatatgaa ttttaccgtc gaggaacgtc ccaacggtga gcacgacggc tttggcacgg
     2161 aacttcagtc ccatttgggt aacagcaccg accacacgat cgttttcgac aataagatct
     2221 tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc
     2281 tgacggtaga gcacacgatc cgcctgagct cgggtagcgc gaactgccgg tcccttgctt
     2341 gcgtttagta tcctaaactg gatacccgcc tgatcgatcg ctttcgccat cagaccgccg
     2401 agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag
     2461 ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc
     2521 gccgcagcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa
     2581 ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc
     2641 cgggccgtgg attctactca actttgtcgg cttgagaaag acctgggatc ctgggtatta
     2701 aaaagaagat ctatttattt agagatctgt tctattgtta tctcttatta ggatcgcact
     2761 gccctgtgga taacaaggat ccggctttta agatcaacaa cctggaaagg atcattaact
     2821 gtgaatgatc ggtgatcctg gaccgtataa gctgggatca gaatgagggg ttatacacaa
     2881 ctcaaaaact gaacaacggt tgttctttgg ataactaccg gttgatccaa gcttcctgac
     2941 agagttatcc acagtagatc gcacgatctg tatacttatt tgagtaaatt aatccacgat
     3001 cccagccatt cttctgccgg atcttccgga atgtcgtgat caagaatgtt gatcttcagt
     3061 gtttcgcccg tctgttttgc accggaattt ttgagttcag cctcgagttt atcgatagcc
     3121 ccacaaaatg tgtcatattc acgactacca ataccgattg cgccaaagcg gaccgcagaa
     3181 agatcgggct tctgttcctg caatgcttca tagaaaggag aaaggttgtc cggaatatct
     3241 ccggcaccgt gggtggagct gataaccagc cagatccccg aggcagataa atcttccaac
     3301 agcggaccgt gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt
     3361 tctgctacat attcggcacc gccgagggtg ctgccgctga taagagttat atctgccata
     3421 aaccgccacc tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg
     3481 tggaaaagta tgggattaaa aaagccgatc agggcttgat ggtacgcatg atcgggttct
     3541 gcaggacgat cagtgtctcg gtggactgaa tttcatcaat tgtttggatc ttgttgataa
     3601 gtacatgctg gagagcatcg atcgaacggc acatcacttt tataaagatg ctgtagtggc
     3661 cggtggtgta gtacgcctcg gtcacttcat cgaggctttc cagctttgcc agcgcggaag
     3721 ggtagtcttt ggcgctcttt aatataatgc cgataaagca gcctacgtca taaccgagct
     3781 gcttcgggct gacatcaata cgcgccccgg taatgatccc cgcctgcttc attttctcta
     3841 ctcgaacgtg aatcgtcccc ggactgacgc caaattgttt cgccagttcg gcgtaagcgg
     3901 tgcgcgcatt gcccattaat gcttccagga tgccacggtc cagattgtcg atcagataat
     3961 tttccatagg attttcttat gcggattgat gattcattct attttagcct tcttttttaa
     4021 tgaatcaaaa gtgagttagg ctttttattg aatgattatt gcatgtgtgt cggtttttgt
     4081 tgcttaatca taagcaacag gacgcaggag tataaaaaat gaaaaccgct tacattgcca
     4141 aacaacgcca aattagcttc gtgaaatctc acttttctcg tcaactggaa gaacgtctgg
     4201 ggctgatcga agtccaggcg ccgattctta gccgtgtggg ggatggcacg caggataact
     4261 tgtcgggctg tgaaaaagcg gtacaggtaa aagtgaaagc tctgcctgat gcccagttcg
     4321 aagtggttca ttcactggcg aagtggaaac gtcagacctt agggcaacac gacttcagcg
     4381 cgggcgaagg gctgtacacg cacatgaaag cccttcgccc cgatgaagac cgtctttctc
     4441 cgttgcactc ggtctatgtt gaccagtggg actgggaacg cgtaatgggc gacggtgagc
     4501 gtcaattctc gactctgaaa agcacggtag aggcgatttg ggcgggaatt aaagcaaccg
     4561 aagctgcggt tagcgaagag tttggcctgg caccgttcct gccggatcag atccacttcg
     4621 tacacagcca ggagttactg tctcgttatc cggatcttga tgccaaaggg cgtgagcggg
     4681 cgatagcgaa agatcttggt gcggtattcc ttgtcgggat tggcggcaag ctgagcgatg
     4741 gtcatcgcca cgacgtgcgc gcaccggatt atgatgactg gagcaccccg tcagagctgg
     4801 gccatgcggg tctgaacggc gatattctgg tgtggaaccc tgtactggaa gatgcgtttg
     4861 agctttcttc catggggatc cgcgtggatg ccgacacgtt gaagcatcag ttggcgctga
     4921 ccggtgatga agatcgtctg cagctggaat ggcatcaggc gttgctgcgc ggtgaaatgc
     4981 cgcagaccat cggcggcggt atcggccagt ctcgtttgac catgctgctg ctgcaactgc
     5041 cgcatatcgg ccaggtccag tgtggagtat ggccagctgc tgttcgcgag agcgtccctt
     5101 ctctgctgta ataatttatc gccgccagcg tctgagcagg cggcttcgca tcccggtatc
     5161 aaagcgccag atatgatcga aaatgcgcat gatgccgggt ttgccgtgtg ccgacatcgc
     5221 cacggcatga aagcgatgct gatgtacccg ctgcagctct ttcactttac tcgtcacgtc
     5281 gtcaggcaac cgctgggcga taaaatcaga aatcaccacc gcatcggcat caaaccattc
     5341 cctgctttgc aagcgttcca taatggcgcg aaaacaactg gcaagatcgg tgccgccacg
     5401 aaactgctgg cttaaaaaac ggattgcttg ttcgatgcct tgtgggcctg aaagctcata
     5461 acggacgatc tcggtggaaa atagcataat ataacagcgc cggttttcag cgagagcaat
     5521 gcgcatcaaa gccaggcaga acgctttcgc acactgttca ttaaagccgc ccattgagcc
     5581 ggaagtatcc acgcagacaa taaacggtcc gcgcggctgt tcgtcgtaat ctttatgcac
     5641 caccgggcgt tcgatcactt tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa
     5701 ctgtttttcc accagccgac ggtaaaactc atactccagt tccgttatcc ctagtgtcgc
     5761 cagttctggc ggcagaagac gtaaaatatc atcgctttgt tgcagaccat caacctgctc
     5821 aggaaccgtc gccggttcgc gcaccatggt gcggaaggtt tccatctgcg catcgttgcg
     5881 cggtattgat ttggcttccc gggaacgccc cagttgttct gccaggcgtt tcagttccgg
     5941 ctgttcgtta agaaattcac cgtatttcac aatcaactga tagtcgccac gtttaagctg
     6001 accggcgctc atatcccaca gacgaccagc tgcggtattg ttatctgcga gaatcggttc
     6061 aagttgtccg ctcagcgtca tgcgttcctg aacttcactc aacagttgtt cgcgttcttc
     6121 ttctaatagc tgttgattta acgttgttgc ttgcacgatc agacttaaac gccagcgctg
     6181 gagaaaaagc gtgtgtaacg ctgaagtgat cgtgctgtta gcatcgacca actgtcgggc
     6241 ttgttctgcc catggggaat tcagacgatg cagtaagtcc aggatctgtg gtagctgcac
     6301 aataaactgc ggcgtggaga ggagctggct ttgctgatag cacatcacct cttcggtgag
     6361 ttccggcggg actcgggcat ctttcagccg actgcgcagc gcctcacgcc agcggggaac
     6421 atcatcagtg attgccgcct tcagtcgtgg gaatttttca aagaagactg ccagctgcgg
     6481 tgaggccagc agcgcgatga tcatctcttc gatcaatccc tcttcgctga cggccagcat
     6541 cacattaagc gtatccagcg ttagcattgt tgtgcctggc gaatctgtgc gccgacatcc
     6601 tgtaggctgg cttcaatgcg acctaaccag tcgccaggga taaacagaca tctttgctgt
     6661 tcgctgaata aagcgtgttg cttgcgccag tcgctttcaa gctcttccaa ttgttgtttt
     6721 atttcacctg gcagaccttc agccgatgaa ccgggaagtg ccagcgtact gccttgtaaa
     6781 cttacatcgc gtacaacaag atgttgggcg ctatcaactt ccagattcag tttctgggca
     6841 aagccgatac cgttcagttt gccgcgaatt tcaccacctt tgctcagcca ctgttccagc
     6901 gcgctacgct caaaggagat atgaaccact tccatattat gcaattttaa cggtttttgc
     6961 agcagcagag tcagagtgga agcagtaacg ttaacaggaa gttgatactg ctgacgacgg
     7021 ctgaaaatgc cgcccagacg aattaccgtt aaggctgttt tatcgctttg ttgctgctgg
     7081 agttgcaggt gacgttgcac aatcgcgccc aggcgggtca acatcccttg ctgttgccag
     7141 gcatgaccgg tcatcaatac atcaatttgt tgttgtatca aattcaggct ttgcgcgtca
     7201 taccacaggc aatctttcag caaaatgaga tctaccgggg caacagcact gcgaccgcta
     7261 aaaaaggcgc tggcctgcaa taatcgaatc gcttttttcc agcgacgatc cgagacataa
     7321 ggcgcatccg gtaatttatc cagttgctgg cgcagcataa aaatcagctc aaatacatga
     7381 tcgggcagcg taatttcacc aatctctttt tgccagcgtt catattcttc atctgtgacc
     7441 tgcaaggagg ctggaaccgg attgtcattt tcatcctgtt gactggtcag catggagcgg
     7501 aaattcgctt tatcctgcac tttatctaac cacagacgaa tcagcatgcg gtcatataac
     7561 gcttccagac tgctgtctgc ttccggcagc tcgttggagg ccgccaccag caggcgcatc
     7621 gggatttttt ctacgtgtgc accgttgcgg aactggcgct cgttaatggc ggtgagcagg
     7681 gtattaagaa ttgccggacc cgctttccag atctcatcca gaaagacgat ttctgcttcc
     7741 ggcaggtaac cgctggttaa acgttcatag cgcccttcat cttttagcgc ctgaatagaa
     7801 aggggaccaa aaacttcttc cggcgtggag aagcgggtca tcagatattc aaacgcgcgg
     7861 gcattctgaa aggcgaattt taagcgccgg gcgatcaaac ttttggcaat acctggcggg
     7921 ccaaggagga acacactttc accacttaat gccgctaata aacacaagcg gatggcgtgg
     7981 ctacgttcat aaagcccctt ttccaacgaa ctgctcaggc gggaaattct ttccgctaat
     8041 aaatgagggt gagccataat gaagtggcgt cctttcgtca aaagttctgc gtaaattgcg
     8101 agtatagacg tttcttgctg gtggctaaaa tagtctcaaa gggagggtat ttttctttga
     8161 gccaggttaa tgtggccgca tttaggagta cgattttgcc gttaatcgtg catactgtgc
     8221 gcttttttgt gggccaaggg actaagcaca catttcatat ttcaacgaaa gactagtcta
     8281 tgagcactga taataagcaa tcattgcccg cgattaccct cgcggcgatt ggagttgtct
     8341 acggcgatat tggtaccagc ccgttatata cacttcgtga atgtttgtcc ggccagtttg
     8401 gttttggcgt tgaacgcgat gccgtgtttg gctttttatc gctgatcttc tggctgctaa
     8461 tctttgtggt ttccattaaa tatctcacct tcgtgatgcg ggcagataac gccggtgaag
     8521 gggggatcct gacgttgatg tcgcttgccg ggcgtaatac gtcggcgcga accacatcaa
     8581 tgctggtgat tatggggcta atcggcggca gctttttcta tggtgaagtc gtcataacac
     8641 ccgctatttc ggtgatgtca gccattgaag gtctggaaat cgtcgccccg cagctggata
     8701 cctggatagt tcccctctca attatcgttc tcactttatt atttatgatt caaaagcatg
     8761 gcacggctat ggtcggtaag ctgtttgcgc cgatcatgct gacctggttt ttgattctgg
     8821 caggactggg gttacgtagc attattgcta accaggaagt gctgtatgcg ctgaatccga
     8881 tgtgggcggt gcatttcttc cttgaataca aaacggtttc ttttattgca ttaggggcag
     8941 tggtgctgtc gattacgggg gtcgaggcgc tgtatgctga tatggggcac tttggtaagt
     9001 tccctattcg cctggcgtgg ttcaccgtcg tattaccttc cttgaccctt aattacttcg
     9061 gtcagggagc gctgttgtta aagaatccgg aagcgattaa gaacccgttc ttccttttgg
     9121 caccggactg ggcgctgatc ccgctgctga tcatcgccgc actggcgacg gtaatcgcct
     9181 cgcaggcggt tatctctggc gtcttttcat tgacgcgtca ggcggtacgt ctgggatatt
     9241 tgtcgccgat gcgcattatt cacacctccg aaatggagtc agggcaaatc tatattccgt
     9301 ttgtgaactg gatgctctat gtcgcggtcg tgattgtgat tgtcagcttt gagcactcca
     9361 gcaacctggc ggcggcgtac gggattgcgg tgaccggaac catggtgctg acgtctattc
     9421 tctcgactac cgtggcacgt cagaactggc actggaataa gtattttgtt gcgctgatct
     9481 tgattgcttt cctttgtgtc gatattccat tgttcaccgc taacctcgat aaactgctct
     9541 ccggcggctg gttgccattg agcctcggta cggtgatgtt tatcgtgatg accacctgga
     9601 agagtgagcg tttccgcttg ctgcggcgga tgcatgaaca tggtaactct ctggaagcga
     9661 tgattgcttc gctggagaaa tcaccgcctg ttcgcgtgcc cgggaccgcg gtatatatgt
     9721 cgcgtgcaat caacgtcatt ccctttgcgc tgatgcataa ccttaaacat aacaaggtat
     9781 tgcatgagcg ggtgattctg ttaactctgc gcaccgaaga cgctccatat gtccataacg
     9841 tccgtcgggt acagattgaa caactgtcgc ccactttctg gcgcgtggtg gcaagttatg
     9901 gttggcgaga aacgccaaac gtggaagaag ttttccaccg ctgcggtctg gaaggattaa
     9961 gttgccggat gatggaaacc tccttcttta tgtcgcatga gtcgttgatc ctcggcaaac
    10021 gcccgtggta tttgcgtctg cgcggcaagc tgtacttgct gctgcaacgt aatgcgctgc
    10081 gtgcaccaga tcaatttgaa atcccgccaa acagggttat cgaactgggt actcaggtcg
    10141 aaatctaacg ccagacgcct cctttcttca taagggggcg tttttgtttt catggttaat
    10201 caccatgtaa aacgtttcga ggttgatcac atttccgtaa cgtcacgatg gttttcccaa
    10261 ctcagtcagg attaaactgc gggtcagcga aacgtttcgc tgatggagaa aaaaatgaaa
    10321 aaaggcaccg ttcttaattc tgatatttca tcggtgatct cccgtctggg acataccgat
    10381 acgctggtgg tgtgtgatgc tggtttaccc atccccaaaa gtacaacgcg tatcgatatg
    10441 gcattaaccc agggtgtacc ttcttttatg caggtgctgg gcgtcgtcac aaatgaaatg
    10501 caggtcgagg ctgccattat cgcggaagag atcaaacacc ataatccgca actccacgaa
    10561 acgttgctca ctcaccttga gcagctgcaa aaacaccagg gaaataccat tgaaattcgt
    10621 tacaccacgc atgaacaatt caaacaacaa accgcagaaa gtcaggcggt aattcgcagc
    10681 ggagaatgtt ctccgtatgc gaatatcatt ctctgtgctg gcgtgacgtt ctgaggccgt
    10741 catggaagca ttacttcagc ttaaaggcat cgataaagcc ttcccgggcg taaaagccct
    10801 ctcgggcgca gcgttaaatg tctatccggg ccgcgtgatg gcgctggtgg gcgaaaacgg
    10861 cgcgggtaaa tccaccatga tgaaagtgct tactggcatc tatactcgcg atgccggtac
    10921 gcttttatgg ctggggaaag aaacgacatt taccgggccg aaatcttccc aggaagccgg
    10981 gattgggatt atccatcagg aactgaacct gatcccgcag ttaaccattg ccgaaaacat
    11041 tttcctcggt cgtgagtttg ttaatcgctt tggcaaaatt gactggaaaa ccatgtatgc
    11101 cgaagcggat aaattgctgg ctaaacttaa cctgcgcttt aaaagcgaca agctggtggg
    11161 cgatctttcc atcggtgacc agcaaatggt tgaaatagcc aaagtgctga gctttgagtc
    11221 gaaagtcatc attatggatg aaccgaccga tgcgctgacc gataccgaaa ccgaatccct
    11281 gttccgcgtc atccgcgagc tgaaatcgca aggccgcggt attgtctata tctcccaccg
    11341 catgaaagaa atcttcgaga gttgcgatga cgttaccgtt tttcgtgacg gacaatttat
    11401 tgctgagcgc gaagtggcat cactgaccga agattcgctg attgagatga tggtgggtcg
    11461 caagctggaa gatcaatatc cgcacctgga caaagcgccg ggagatatcc gcctgaaagt
    11521 cgataatctc tgcggacctg gcgttaacga tgtctctttt actttacgca aaggcgaaat
    11581 tcttggcgtc tctggtttga tgggcgcagg tcgtaccgaa ctgatgaaag tgctctacgg
    11641 cgcactaccg cgcaccagcg gttacgtcac cctggatggg catgaagtcg ttacccgttc
    11701 accgcaggat ggcctggcaa acggcattgt gtatatctcc gaagaccgta aacgtgacgg
    11761 tttagtgttg ggcatgtcag taaaagagaa catgtcgctg acagcgctgc gctacttcag
    11821 ccgcgctggc ggcagtttga agcatgccga tgaacagcag gctgtgagtg attttattcg
    11881 cctgttcaat gtgaaaacgc cgtcgatgga gcaggcaatt ggtctgcttt ccggtggcaa
    11941 tcagcaaaaa gtggcgattg cccgcggtct gatgacacgc cccaaagtgt tgatccttga
    12001 tgaacctacc cgtggcgtag atgtcggcgc gaaaaaagag atctatcaac tgattaacca
    12061 gttcaaagcc gatggcttga gcatcattct ggtgtcatcg gagatgccag aagtattagg
    12121 catgagcgat cgcatcatcg tcatgcatga agggcatctc agcggggaat ttactcgtga
    12181 gcaggccacc caggaagtgt taatggctgc cgctgtgggc aagcttaatc gcgtgaatca
    12241 ggagtaaaaa aatgacaacc cagactgtct ctggtcgccg ttatttcacg aaagcgtggc
    12301 tgatggagca gaaatcgctt atcgctctgc tggtgctgat cgcgattgtc tcgacgttaa
    12361 gcccgaactt tttcaccatc aataacttat tcaatattct ccagcaaacc tcagtgaacg
    12421 ccattatggc ggtcgggatg acgctggtga tcctgacgtc gggcatcgac ttgtcggtag
    12481 gttctctgtt ggcgctaacc ggcgcagttg ctgcatctat cgtcggcatt gaagtcaatg
    12541 cgctggtggc tgtcgctgcc gctctcgcgt taggtgccgc aattggtgcg gtaaccggag
    12601 tgattgtagc gaaaggtcgc gtccaggcgt ttatcgctac gctggttatg atgcttttac
    12661 tgcgcggcgt gaccatggtt tataccaacg gtagcccagt gaataccggc tttactgaga
    12721 acgccgatct gtttggctgg tttggtattg gtcgtccgct gggcgtaccg acgccagtct
    12781 ggatcatggg gattgtcttc ctcgcggcct ggtacatgct gcatcacacg cgtctggggc
    12841 gttacatcta cgcgctgggc ggcaacgaag cggcaacgcg tctttctggt atcaacgtca
    12901 ataaaatcaa aatcatcgtc tattctcttt gtggtctgct ggcatcactg gccgggatca
    12961 ttgaagtggc gcgtctctcc tccgcacaac ccacggcggg gactggctat gagctggatg
    13021 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
    13081 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
    13141 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
    13201 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
    13261 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
    13321 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
    13381 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
    13441 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
    13501 tgattaaccc gaccgactcc gacgcagtag gtaatgctgt gaagatggct aaccaagcga
    13561 acatcccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
    13621 cttctgataa cgtactgggt ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
    13681 aaggtgccaa agttatcgag ctgcaaggca ttgctggtac atccgcagcc cgtgaacgtg
    13741 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagccag
    13801 cagattttga tcgcaccaaa ggtttgaacg taatgcagaa cctgttgacc gctcatccgg
    13861 atgttcaggc tgtattcgcg cagaatgatg aaatggcgct gggcgcgctg cgcgcactgc
    13921 aaactgccgg taaatcggat gtgatggtcg tcggatttga cggtacaccg gatggcgaaa
    13981 aagcggtgaa tgatggcaaa ctagcagcga ctatcgctca gctacccgat cagattggcg
    14041 cgaaaggcgt tgaaaccgca gataaagtgc tgaaaggcga gaaagttcag gctaagtatc
    14101 cggttgatct gaaactggtt gttaagcagt agttttaatc aggttgtatg acctgatggt
    14161 gacataaata cgtcatcgac agatgaacgt gtaatataaa gaaaagcagg gcacgcgcca
    14221 ccctaacacg gtggcgcatt ttatggacat cccgaatatg caaaacgcag gcagcctcgt
    14281 tgttcttggc agcattaatg ctgaccacat tcttaatctt caatcttttc ctactccagg
    14341 cgaaaccgta accggtaacc actatcaggt tgcatttggc ggcaaaggcg cgaatcaggc
    14401 tgtggctgct gggcgtagcg gtgcgaatat cgcgtttatt gcctgtactg gcgatgacag
    14461 cattggcgag agcgttcgcc agcagctcgc cactgataac atcgatatta ctccggtcag
    14521 cgtgatcaaa ggcgaatcaa caggtgtggc gctgattttt gttaatggcg aaggtgagaa
    14581 tgtcatcggt attcatgccg gcgctaatgc tgccctttcc ccggcgctgg tggaagcgca
    14641 acgtgagcgt attgccaacg cgtcagcatt attaatgcag ctggaatcac cactcgaaag
    14701 tgtgatggca gcggcgaaaa tcgcccatca aaataagact atcgttgcgc ttaacccggc
    14761 tccggctcgc gaacttcctg acgaactgct ggcgctggtg gacattatta cgccaaacga
    14821 aacggaagca gaaaagctca ccggtattcg tgttgaaaat gatgaagatg cagcgaaggc
    14881 ggcgcaggta ctgcatgaaa aaggtatccg tactgtactg attactttag gaagtcgtgg
    14941 tgtatgggct agcgtgaatg gtgaaggtca gcgcgttcct ggattccggg tgcaggctgt
    15001 cgataccatt gctgccggag atacctttaa cggcgcgtta atcacggcgt tgctggaaga
    15061 aaaaccattg ccagaggcga ttcggtttgc ccatgctgcc gctgcgattg ccgtaacacg
    15121 taaaggcgca caaccttccg taccgtggcg tgaagagatc gacgcatttt tagacaggca
    15181 gaggtgacgc ttggctacaa tgaaagatgt tgcccgcctg gcgggcgttt ctacctcaac
    15241 agtttctcac gttatcaata aagatcgctt cgtcagtgaa gcgattaccg ccaaagttga
    15301 agcggcgatt aaagaactca attacgcgcc atcagctctg gcgcgtagcc tcaaactcaa
    15361 tcaaacacat accattggca tgttgatcac tgccagtacc aatcctttct attcagaact
    15421 ggtgcgtggc gttgaacgca gctgcttcga acgcggttat agtctcgtcc tttgcaatac
    15481 cgaaggcgat gaacagcgga tgaatcgcaa tctggaaacg ctgatgcaaa aacgcgttga
    15541 tggcttgctg ttactgtgca ccgaaacgca tcaaccttcg cgtgaaatca tgcaacgtta
    15601 tccgacagtg cctactgtga tgatggactg ggctccgttc gatggcgaca gcgatcttat
    15661 tcaggataac tcgttgctgg gcggagactt agcaacgcaa tatctgatcg ataaaggtca
    15721 tacccgtatc gcctgtatta ccggcccgct ggataaaact ccggcgcgcc tgcggttgga
    15781 aggttatcgg gcggcgatga aacgtgcggg tctcaacatt cctgatggct atgaagtcac
    15841 tggtgatttt gaatttaacg gcgggtttga cgctatgcgc caactgctat cacatccgct
    15901 gcgtcctcag gccgtcttta ccggaaatga cgctatggct gttggcgttt accaggcgtt
    15961 atatcaggca gagttacagg ttccgcagga tatcgcggtg attggctatg acgatatcga
    16021 actggcaagc tttatgacgc caccattaac cacgatccat caaccaaaag atgaactggg
    16081 ggagctggcg attgatgtac tcatccatcg gataacccag ccgacccttc agcaacaacg
    16141 attacaactt actccgattc tgatggaacg tggttcggct tagatttacg ctgtcttttg
    16201 atcaaattat taccatcggt tgttttcaga agcatgaaca ttgctgctga agcaacagta
    16261 ataatgccca ttgtgataaa cgtatagtgg aattgttcga cagtcgttgt gccttccatt
    16321 ccttcataaa cgcgaaggac ggccgcactt acagctacgc ctaaactgat agacagttgc
    16381 tgcgtgaccg ccagaacact gttaccgctg ctggcgttgt catcggtcag atcggcaagt
    16441 gtgatggtat tcatcgcggt aaactgcgtc gacatagcca tccctaatat aaacaacggc
    16501 aagatcagca tccatattgc cattgccggt gattgcaaag agaactgagc gatcattagc
    16561 ccaataatca ccgtgatccc cactaatgta tggcgatagc ccagacgacg taagacttgg
    16621 gtaaccatcg attttgcaat aatggaacct aacgctgtcg gtgccatcat acagccagca
    16681 ataaacgcct gataaccaaa tcctacctgt aacattaatg gcataaggaa cggtacacag
    16741 ccggtcccca gacgggttgc aatattgcct acgataccga tcgagaaagt gcgggtttta
    16801 aataaatcta atgaaattaa tgggtttggc gtgcgtcgtg catggagaat atagagaagc
    16861 agtaacccga tgctggtgac aattaccgtc aaggcaatcc agctggcgac aatcttttcc
    16921 ccgaatagct ctattccgct tgagaagaga acaaggctga ggccaaacag caaaaagcca
    16981 gtgatatcga atctgcgtcg tgcggtggtg aaattgggca tatgtttgcg cgcgtaaaga
    17041 aggcccgcaa tacctatggg gatattgatt aaaaatatcc agtgccaggt tgcccaggtg
    17101 accagcacgc cgccaagaat ggggcctaaa attggcccca ccagacccgg catggcgaca
    17161 aaattcaata ctggaagaag ttcattacga ggataagcgc gcagtaaggc cagccgagca
    17221 acaggcatca tcattgcgcc gcctatcccc tgaataaccc ggaagacaac cagctgtggt
    17281 agcgaattag aaagtgcgca ggccagagaa cccaatgtga acagactcac ggcaagggta
    17341 aaaatgcgac gcgtaccgaa gcgatcggct agccatccgc ttaccggaat aagcatcgcc
    17401 accgtcagcg tataactgat gatggctgat tgcatcgcga gaggagaacg attaaggcta
    17461 tgagcgattg cgggtaaggc ggtattcaga atagtggcat caagtgcctg catgaagaag
    17521 gccatcgccg cgatccacgg caaacccgcc atactgcgct tctttttatc gctcattcaa
    17581 tgtcctgtta tcgggttatc acttatcagg tgagcgtagc agcgcctgac aagctttaaa
    17641 tgccgcgtcg ccatcgcttt ggataatcgc atcgactatc gcctgatgca gatccagctt
    17701 tatcactgtg tcgctggtaa ttgacgtgaa gtaagtgtga taaaccgaat ggaatagcga
    17761 ggcgaatgag gtcaaaaacg gattggcgct catttcatag atatgctcat gccaggccat
    17821 atcgacttcg atccagcgtt cacggcgaaa gttctctttt aatgccgcca tttcggccat
    17881 taacgtattg agatgcgctt tctgttccgc ggtgccaacc gttgctgcca gtaggcaagc
    17941 ttgtggctcc agacagatac gcataaccag aaagtgatcg atgacctgat gaaagttctc
    18001 ttctgtcatc caccaggtaa gcaattcctg atcaagaaaa ttccagttcg attgtggcat
    18061 gacccgggta ccaattcgcg gtcgcggtaa aaccatccct tttgccgtta acgttttgac
    18121 cgcttcgcgt accgctgtac gactcactcc aaattgctcg cccagctcaa tctcaccggg
    18181 caaaatggtg ccgggttcat attcaccttt taagatccgt tgcgccagct tctcagccag
    18241 aacatacgaa aggtttttct gtgcagctaa ctgttgtgcg cttaaaggca ttactaatct
    18301 tcctttttct ttttattcct ccttagtatg ccaccaggaa gtgtgattac ggttgcaaaa
    18361 acggcaaatt gcttgtttta tggcacatta acggggcttt tgctgaaaaa atgcgcggtc
    18421 agaaaattat tttaaatttc ctcttgtcag accggaataa ctccctataa tgcgccacca
    18481 ctgacacgga acaacggcaa gcacgccgcc gggtcagcgg ggttctcctg agaattccgg
    18541 cagagaaagc aaaaataaat gcttgactct gtagcgggaa agcgtattat gcacaccccg
    18601 cgccgctgag aaaaagcgaa gcgg
//
LOCUS       DAZFKY010000087        18607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFKY010000087 DAZFKY010000000
VERSION     DAZFKY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(10..198)
                     /gene="bcsR"
                     /locus_tag="ACHKCL_002680"
     CDS             complement(10..198)
                     /gene="bcsR"
                     /locus_tag="ACHKCL_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5763628.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            471..2042
                     /gene="bcsE"
                     /locus_tag="ACHKCL_002681"
     CDS             471..2042
                     /gene="bcsE"
                     /locus_tag="ACHKCL_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5763629.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2039..2230
                     /gene="bcsF"
                     /locus_tag="ACHKCL_002682"
     CDS             2039..2230
                     /gene="bcsF"
                     /locus_tag="ACHKCL_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5763630.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2227..3906
                     /gene="bcsG"
                     /locus_tag="ACHKCL_002683"
     CDS             2227..3906
                     /gene="bcsG"
                     /locus_tag="ACHKCL_002683"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5763631.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3993..4100)
                     /gene="ldrD"
                     /locus_tag="ACHKCL_002684"
     CDS             complement(3993..4100)
                     /gene="ldrD"
                     /locus_tag="ACHKCL_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5763632.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            4576..5847
                     /gene="yhjV"
                     /locus_tag="ACHKCL_002685"
     CDS             4576..5847
                     /gene="yhjV"
                     /locus_tag="ACHKCL_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5763633.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            complement(5877..6881)
                     /gene="dppF"
                     /locus_tag="ACHKCL_002686"
     CDS             complement(5877..6881)
                     /gene="dppF"
                     /locus_tag="ACHKCL_002686"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5763634.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(6878..7861)
                     /gene="dppD"
                     /locus_tag="ACHKCL_002687"
     CDS             complement(6878..7861)
                     /gene="dppD"
                     /locus_tag="ACHKCL_002687"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5763635.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(7872..8774)
                     /gene="dppC"
                     /locus_tag="ACHKCL_002688"
     CDS             complement(7872..8774)
                     /gene="dppC"
                     /locus_tag="ACHKCL_002688"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5763636.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(8784..9803)
                     /gene="dppB"
                     /locus_tag="ACHKCL_002689"
     CDS             complement(8784..9803)
                     /gene="dppB"
                     /locus_tag="ACHKCL_002689"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5763637.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(9954..11561)
                     /gene="dppA"
                     /locus_tag="ACHKCL_002690"
     CDS             complement(9954..11561)
                     /gene="dppA"
                     /locus_tag="ACHKCL_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5763638.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(12306..12382)
                     /locus_tag="ACHKCL_002691"
     tRNA            complement(12306..12382)
                     /locus_tag="ACHKCL_002691"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12346..12348),aa:Pro,seq:cgg)
     gene            complement(12474..14165)
                     /gene="eptB"
                     /locus_tag="ACHKCL_002692"
     CDS             complement(12474..14165)
                     /gene="eptB"
                     /locus_tag="ACHKCL_002692"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5763639.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(14420..14950)
                     /gene="lpfE"
                     /locus_tag="ACHKCL_002693"
     CDS             complement(14420..14950)
                     /gene="lpfE"
                     /locus_tag="ACHKCL_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO5763640.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(14955..16010)
                     /locus_tag="ACHKCL_002694"
     CDS             complement(14955..16010)
                     /locus_tag="ACHKCL_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5763641.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(16025..18598)
                     /gene="lpfC"
                     /locus_tag="ACHKCL_002695"
                     /pseudo
     CDS             complement(16025..18598)
                     /gene="lpfC"
                     /locus_tag="ACHKCL_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
BASE COUNT         4459 a         4954 c         4580 g         4614 t
ORIGIN      
        1 accaatcccc tacttttgtt gcgcaaactc tgccagcaac ggccagcgtt ttaatgccgc
       61 ggccaactgt tcgcgttggg aaatatcggc ataatcaata ccaggcagtg aaaatgcctg
      121 ctttaacgcc aaaatatcat tctggaagat atagcctatc gcgggatcag gcagagtatc
      181 tggttcattg ttattcattt tgcttgatcc ctatgaaata accgtaccgt tcatgagaca
      241 attctaaccg tgagcatttc gccggtttat tgtttacggc gagacgggaa tttgctcact
      301 ttttgacttt ccgttacgca acagaaaaag ttaaaaacga aaagactaaa atcacacgtc
      361 ttgtgacctg ctaaattgat tcacatgcta aatctgataa gttttaattt caatggtagg
      421 tttatttctt agctttcgct agtaaactga taatcagaca aaatggtgac atgagggaca
      481 ttgtggaccc tgtattctct atcggtatct catcattatg ggatgagctg cgacatatgc
      541 cagcaggcgg cgtctggtgg tttaacgtcg atcgccatga agatgctatc agtctggcga
      601 atcaaacaat tgcatcccag gctgaaaccg cacacgtcgc ggtcattagc atggacagcg
      661 atccggcgaa aatctttcaa ttagatgatt ctcaagggcc ggaaaaaata aaattatttt
      721 caatgctaaa tcatgaaaaa ggtctatact atttggcccg tgatttgcag tgttctattg
      781 atccccataa ttaccttttt attcttgttt gcgcaaataa cgcatggcaa aacattcctg
      841 ccgagcggct gcgctcatgg ttggataaaa tgaataaatg gagcaggtta aaccattgtt
      901 cgcttttggt aattaatccc ggaaataata acgataaaca attttcattg ttgcttgagg
      961 aataccgttc actttttggt cttgccagtt tgcgttttca gggcgaccaa catttgctgg
     1021 atattgcctt ctggtgcaac gaaaaagggg tcagcgcccg tcagcagctt agcgttcagc
     1081 aacaaaatgg tatctggaca ttagttcaaa gcgaagaggc ggagatccaa ccacgcagcg
     1141 acgaaaaacg cattctgagt aatgttgctg tactggaagg tgcgccgccg ctatcggaac
     1201 actggcaact gttcaacaat aacgaagtcc tgttcaatga agcccgtacc gctcaggcgg
     1261 cgacggtggt cttttcttta cagcaaaatg cgcaaatcga gccactggcc cgcagcattc
     1321 ataccctgcg tcgccagcgc ggtagtgcga tgaaaatcct cgtgcgggaa aataccgcta
     1381 gcctgcgcgc caccgatgaa cgtttgttat tggcctgcgg tgcaaatatg gttattccgt
     1441 ggaatgcgcc actctcccgt tgtctgacga tgatcgaaag cgtgcaaggg cagaagttta
     1501 gtcgctatgt gccggaagat atcactacct tgctgtcaat gacccagccg ctcaaactgc
     1561 gtggtttcca gaagtgggat gtgttctgta atgccgtcaa caacatgatg aataaccctc
     1621 tattacctgc ccacggtaaa ggcgttctgg ttgccctacg tccggtaccg ggtatccgcg
     1681 ttgaacaagc cctgacgctg tgtcgcccta accgtaccgg cgatatcatg accattggcg
     1741 gtaatcggct ggtgctgttt ctctcattct gtcggattaa cgatctggat accgcgttga
     1801 atcatatttt cccattgcct actggcgaca ttttctcaaa ccgtatggtc tggtttgaag
     1861 atgatcaaat cagtgccgag ctggtgcaga tgcgcctgct tgccccagaa caatggggca
     1921 tgccgctgcc tttaacgcaa agttctaaac cggtcatcaa tgccgagcac gatggtcgcc
     1981 actggcgacg aataccagaa cccatgcgac tgttagatga tgctgtggag cgctcatcat
     2041 gatgaccatc agcgatatca ttgaaattat tgtcgtttgc gcactgatat ttttcccgct
     2101 gggctatctg gcgcggcact ctttgcgacg cattcgcgac accttacgtt tgttctttgc
     2161 taaacctcgt tatgttaaac cggctgggac gttacgccgc acggaaaaag ccagggcaac
     2221 caaaaaatga ctcaatttac gcaaaatacc gccatgcctt cttccctctg gcaatactgg
     2281 cgcggccttt ccggctggaa cttctatttt ctggttaagt tcggcctgtt gtgggcggga
     2341 tatcttaact tccatccgct cctcaatttg gtgtttgccg cgtttctgct gatgcccatt
     2401 ccgcgctaca gcctgcatcg cttgcgccac tggattgccc tgccgatcgg ctttgctttg
     2461 ttctggcatg acacctggtt gcctggcccg gaaagcataa tgagccaggg ttcgcaggtg
     2521 gcggggttca gtaccgatta tttaatcgac cttgtcacac gctttattaa ctggcagatg
     2581 attggggcca tttttgtttt attagtggcc tggttattcc tgtcacaatg gattcgcatt
     2641 accgtttttg tggttgccat actgctatgg ctgaacgtac ttaccctggc gggaccaagt
     2701 ttctccttgt ggccagccgg acaaccgacg accactgtaa caacgacggg tggtaacgca
     2761 gcggcaaccg ttgcggcgac gggtggcgca ccggtagtgg gtgatatgcc cgcacaaact
     2821 gcaccgccaa caacggcgaa ccttaacgcc tggctgaata atttctataa cgcggaggcg
     2881 aaacgtaaat cgaccttccc gtcttcgctg cccgctgatg ctcagccatt tgaactactg
     2941 gtgattaaca tctgttcgct ttcctggtcg gatatagaag ccgctgggtt gatgtcgcat
     3001 ccactgtggt cgcatttcga tattgagttc aagaacttta actccgccac ctcctacagt
     3061 ggcccggcgg cgatccgttt actgcgcgcc agctgcgggc agacttcgca cactaatctg
     3121 tatcaaccgg caaataacga ctgctatctg tttgataacc tgtcgaaact gggctttacc
     3181 cagcacctga tgatgggaca taacggccag ttcggcggtt ttttgaaaga agttcgcgaa
     3241 aatggcggca tgcagactga attgatggat caaacaaatc tgccggttat tttgctgggc
     3301 tttgatggtt cgccggttta tgacgatacc gccgtgctta accgctggct ggacgttacc
     3361 gaaaaagata aaaacagccg tagtgccacg ttctacaaca cgcttccact gcatgacggc
     3421 aaccattatc cgggcgtcag caaaacagcg gattacaaag cgcgggcgca gaaattcttt
     3481 gatgaactgg acgccttctt tactgaactg gagaaatcgg gtcgtaaagt gatggtggtc
     3541 gtggtgccgg aacacggcgg cgcgctgaag ggcgacagaa tgcaggtatc tggcctacgt
     3601 gatatcccta gcccgtctat caccgacgtc cccgttgggg tgaaattctt cggcatgaag
     3661 gcaccacatc agggggcacc gattgtcatc gaccaaccga gcagcttcct ggctatctcc
     3721 gatctggtgg ttcgcgttct tgatggcaag attttcaccg aagacaatgt tgactggaaa
     3781 aaactcacca gtgggttgcc acaaacagca ccggtctcag agaactcaaa tgcagtagtt
     3841 attcaatacc aggataaacc gtacgttcgc ctgaacggcg gcgactgggt gccttacccg
     3901 cagtaagcac gaaaaaggcc gcagagtttc ccccctgcgg tctggtccgg gcgcaaattg
     3961 ccattacggc agcctgacgc ccgcatgaca cgttacttcc gcttgttcag ccagttcacg
     4021 atcatactgg caagaatgcc agcaatgacc ggagccgcta aatcatgcca gaaggccatg
     4081 cccagctctg cgagcgtcat atagccgcct gtgttgtaat gacaacgttt cgcggctatt
     4141 cttgagtggt ctaggttcaa gattagcccc cgtggtgttg tcaggtgcat acctgcaacg
     4201 tgcgggggtt ttctctctcc agcaaccaat gccaccaggg attaagcccc cgcaacattg
     4261 cgcctcaccg gaacccttcc ggcttgccgc taattttacg attgcttttt actgtcagca
     4321 ctcggcaatc ttcctcattg cgtgatgcct tccagcttaa tttcttaatt tgcaatagtt
     4381 gcagttcata tccgggctga ctccagactg gtagtgaaat gcgcaatttt gtagaattgc
     4441 gcctcctcgc gacctcccca gtgaatctgt tgcctcattt tttacatact gcgtattcga
     4501 cttctccacc tgttgcgcaa gagaaactgg gtttattcat ttttgcgagg ccgacttctt
     4561 tctggacagg actttatgca gcacaacaca ctaccgaaac acgatcagaa attgccgttt
     4621 acacgctacg acttcggctg ggttttatta tgcataggca tggcgattgg tgccggaacc
     4681 gtgctgatgc cagtacaaat tggcttgaag ggcatttggg tatttattac cgcagcgatc
     4741 attgcttatc ctgccacctg ggtagtgcag gacatttatt taaaaaccct ttctgaaagc
     4801 gattcctgta atgactacac cgatattatc agtcattacc tggggaagaa ctggggaatt
     4861 ttcctcgggg ttatctactt tttgatgatt atccacggga tttttatcta ctctctctcc
     4921 gtggttttcg acagcgcctc gtacctgaaa accttcggtt taaccgatgc cgatctttca
     4981 caatctctac tttataaagt cgctattttc gccgtactgg tagcgattgc gtctggtggt
     5041 gaacgattac tgtttaagat ttctgggcca atggtggtgg tcaaagtagg gattattgtc
     5101 gtgttcggtt ttacgatgat cccgcactgg aatttcgcca atataaccgc cttcccgcaa
     5161 gcctccgtct tttttcgcga tgtcctgctt accattccat tttgcttctt ttctgcagta
     5221 tttattcagg tacttaaccc aatgaatatt gcctatcgta aacgggaagc ggataaagta
     5281 ctggcaaccc ggctcgcgct gcgtacccac cgaattagtt atatcacgct catcgcggtg
     5341 atactgtttt ttgccttttc gtttaccttc tcaattagcc acgaagaagc cgtttctgcc
     5401 tttgaacaaa atatctcggc actggcactg gccgcgcagg tgatccctgg gcatatcatt
     5461 catatcacct ctacggtgct gaatatcttt gccgtactga ccgcattctt tggcatttat
     5521 ctcggtttcc acgaggccat taaaggcatt attctcaatc tgttaagccg aattattgat
     5581 accaagaaaa ttaactcacg cgtgctgact ctggcgatct gcgcttttat cgtcattacg
     5641 ttgacgattt gggtttcgtt tcgtgtatcg gtgctggtgt tctttcagtt gggaagcccg
     5701 ttatatggca ttgtgtcgtg cctcattccg tttttcctga tctataaagt cgcacaactg
     5761 gaaaaacttc gcggatttaa aacctggctg attctgcttt acggcatttt gctatgcttg
     5821 tcgccactgt tgaagctgat tgagtaaacc ggagcgcatg gccccggttt tgtgagttaa
     5881 cgctgcggat tttcatcctg atcaacagca aaacaagcta ccagttgacc gccgtagtct
     5941 tttagctgcg gctgcaactg ggtgcagggg ccgaagcgcc gacgacagcg ggcgttaaac
     6001 gcgcagcccg gcggtggatt cagcgggctt ggcagttcac cggtgagttt gatgcgctcg
     6061 cggcgatcgt ccggattcag gcgcggcgtc gcggaaagta gcgcctgagt gtacggatgg
     6121 cgcgggttat tgaagatttg gtctttcgtt cccttctcca cgcagcggcc caggtacatc
     6181 accatcactt catcagcaat gtgctccacc accgacaggt cgtgggagat gaagacataa
     6241 gaaagcccca gttcctgctg caaatccatc atcaggttga gcacctgtgc acgcaccgag
     6301 acgtccagcg cggagaccgg ttcatcggca atcaccacgt ccgggtcgag catcagacct
     6361 cgggcaatgg cgatacgctg acgctgaccg ccggagaaca tatgcggata gcggtcatag
     6421 tgctcggttt tcaggccgac tttcgccatc atcgacaggg ctttttcccg acgctgttct
     6481 ttgcttaagc tggtgttgat cagcagcggc tcttcaagaa tttgcccgat ttttttacgc
     6541 ggattcagcg aaccgtaagg gttctggaag acaatctgga ttttctgccg acgcagcttc
     6601 tgcgcctgcg gatcgtgctt aagcagatcc tgcccctggt aatacagctc gccgccggtg
     6661 ggcgtttcaa tcatcgtcag caaccgaccg agggtcgatt taccgcagcc agattcacct
     6721 actaccgcca gcgttttgcc acgttcaagg ttaaacgaaa cgccgtccag cgccttcacc
     6781 agacgttccg gagcgaacat acctttcttc accggataat gttttttcag gtcgatagcc
     6841 tgcaacagcg gttgttgcga ggtggcctct tgcgtactca tagtgtcggc ctcccggcat
     6901 catcaagtgg gtaatggcat ttggactgac gcccgtcagc gagcatattc agcgccggtt
     6961 cttcagcgcg acatctgtca gtggcatagg ggcagcgcgg gttaagcaag cagccgttcg
     7021 ggcggtcgta cttgccggga acaacgccag gcaacgatgc cagacgttct ttgtcctgag
     7081 caaattccgg cagcgcacgc agcaatgcct gagtatacgg gtgacgcggc gcatggaaga
     7141 tggcgtgcgc atcgcccgtt tccaccacct gaccggcata catcacgatg attttatgtg
     7201 ccgcttccgc caccagcgcc aggtcatggg taattaacac cagcgccatg ttctctttct
     7261 gctgtagctc cagcagtagt tcaatgattt gcgcctgaat ggtcacgtcc agcgcagtgg
     7321 ttggttcatc ggcaatcagc agttttggcc gacaggcaat ggccatcgca atcatcacgc
     7381 gctggctcat gccgccggaa agctgatgcg ggtaaacatc cagacgcgaa gccggatcgg
     7441 gaataccgac ctgattcaac aagtcaatcg cccgctgacg acgagtgctc ttattaccgc
     7501 cctgatgcac cttaatcgct tccataatct ggaaacccac ggtgtagcac gggttaaggc
     7561 tggtcatcgg gtcctggaag atcatcgcca cttcggcacc caccaggttg cggcgctctt
     7621 tttccgagat acgctgcaaa tcctgaccgt taaactccag cttttccgcc atcacgcggc
     7681 ccggataatc aatcagcccc ataatcgcca gcgaactgac cgacttaccg gagccagact
     7741 cacccacaat cccgactact tcgccctgtt ttacgctgta gctaatgcgg tctacggcgc
     7801 ggaacggtgc gctttcgtcg ccgaaatgca ccgataattt atctacattt aataacgcca
     7861 tctcgaacct cttactgctt cagtttggga tcgagcgcgt cacgcagacc gtcacccatc
     7921 aggttaaatg ccagcaccgt cagcaggatc gccagacccg ggaaggtcac gacccaccag
     7981 gcgctttgcg cgaactgcaa cacgtcggag agcatggtgc cccactccgg cgttggcggc
     8041 tgcgccccca tgccgaggaa accaagagca gccatatcga gaatggcgtt agagaaaccg
     8101 agcgacgcct gaacaatcag cggtgcaagg cagttcggga agatgttaat aaacatctgg
     8161 cgcatcgccc cggctcctgc cacgcgagaa gcggtgacgt aatcgcgatt cacctccacc
     8221 agcacggcgg cgcgggttaa gcgcacatag tgcggcaagg caacaaaggt cagcgccagc
     8281 gcggcgttac caatcgacgg gccgaaaatc gccaccagca ccagtgccaa cagcagactt
     8341 ggcagcgcca gcatgatatc gaccacgcgc ataatgatgt tatcgaccag gccgccgaag
     8401 taaccggcga tcaggccgag aataacgccc ataatcagcg ataacaccac caccagacag
     8461 ccaaccagca gcgacaagcg cgcgccgtac atcagccgcg acagcacatc acggcctaca
     8521 tcatcggtac ccagcaagtg cgccatgctg ccgccttcct gccaggctgg cggggcgagc
     8581 agtgcatcgc ggaactgttc cgccgggtta tagggagcaa tccagttggc aaagatcgcg
     8641 ataaacagca cgatgacgac gtaaaccagc ccgacgaccg cgcctttgtt gcgtttaaaa
     8701 tagtgccaga actcctgtaa aggggtcatc ggtaccggcg cgctaatcac tttattttca
     8761 gtaacctgtg acatgatgac cccttacttc ttatgacgaa tacgcgggtt caccacgcca
     8821 tacagcagat cgaccagcag gttgacgagg ataatcatcg tcgccaccag caatacgccg
     8881 ccctgcacca ccggataatc gcgccgttgc agtgcgtcaa tcaaccagcg ccccagaccg
     8941 ggccacgaga agatggtttc ggtcagaatc gcccccgcca gcaatgttcc cacctggagg
     9001 ccgataacgg tcactaccgg cagcatcgcg ttacgcagcg catggacgat aatcacccgc
     9061 atgcgggtca accctttggc gcgcgcggtg cggatgtaat cttcacccag cacttccagc
     9121 atcgaggagc gtgtcatacg cacaatgacc gccagcggaa tagtacccag cacaatggcg
     9181 ggcaagatca tatgggcgac ggcatcgata aagttaccgt cttcacccca gatggcggtg
     9241 tcgatcagca taaaaccggt taacggattg gaatcatcga ggaacaccat atcgctcacg
     9301 cgaccggaga cgggtgtcag gttccagtgc accgaaacca gcatgatcag catcatgccc
     9361 caccagaaga taggcattga ataacctgtc agcgccaggc caaccgctgt gtgatcgaaa
     9421 atggaaccgc gtttaaccgc ggccagcaca ccaaccggaa tgccgaccgc ggtcgcaaaa
     9481 atcatcgcgc agacgccaag ttccagcgtg gcctgaaagc gcggcacgaa ctcttcccat
     9541 accggaattc ggcttttcat tgaaatgcct agatcgccat gcataacgcc ccaaatgtaa
     9601 tggagatact gctgccacat cggtttatct aagccgagtt cagccagcaa ctgcgcgtga
     9661 cgctctgggg agatcccacg ttcgcccgcc atgatcatca caggatcgcc cgggatcatg
     9721 tggacaaagg caaatgtgag aagggtaata ccgataaacg tggggatgac gagtcccaaa
     9781 cgtcggagaa taaactgcaa cataacccgg attctctgta gtgacccgca gcctggaact
     9841 gcgtgtctgt attgctcaca aatcaattcc cgcccaggtc gggcagggaa tgaagtattg
     9901 ctgccggatg gcgctgcacg cagaggcaca gcaccaccgg gtatggcttt taattattcg
     9961 atagagacgt tttcgaagtg atgtttgcct aatggatcaa ccacatagcc tttaacttct
    10021 ttgcgtaccg gttcaaacac ggtggagtga gcgatgatca gtgccggagc ctgatcgtgc
    10081 atcaccacct gcgcttgctt gtacagttca acgcgtttgt tatggtcgtc ggtagcacgc
    10141 gccggttgaa tcagatcttc aaacggtttg tagcaccatt ttgagtagtt ggagccttgt
    10201 tcagaggcgg cgcagctgaa cagagtggcg aagaagttat ccggatcccc gttatcgcca
    10261 gtccagccca tcattaccgt ctggtgctcg ccatctttcg cacgcttgag gtactcaccc
    10321 cattcgtagg tgacaatttt ggcctgcacg ccgactttcg cccagtctgc ctgaatcatc
    10381 tccgccatgc ggcgagcgtt cgggttatac ggacgttgta ccggcatcgc ccacaggtcg
    10441 atggagaaac ctttttccag acccgcttct ttcagcaagg ctttcgcttt ttcaggatcg
    10501 taggtgtagt cctgaacgtc gtcgttatag ccccacatgg ttggcgggat caggtttttc
    10561 gctgatacac ccgcgccctg ataaaccgct ttgatgatcg cgtctttgtt caccgcgtag
    10621 gtcagagcct ggcgaacttt cacgtcatcg agtggttttt tctgcacgtt atacgagaga
    10681 taaccgacgt tcagccccgg catttccatc aggttgatag atttatcctg cttcatgcgg
    10741 gcgatatctg ccgggttcgg gtacggcatc acctggcatt catttttctg caatttagcg
    10801 taacgcacgg aagcgtcagg ggtaatagag aaaaccagcg tatcgatctg cggtttggtg
    10861 ccccagtagc catcaaacgc tttgtagcgg atacgggaat ctttttgata ctgctgtaac
    10921 tggaacggac cggtgccgat tgggttgagg tccagttttt ccggtgtacc ggctttcatc
    10981 atcgcatcag catattcttt tgacagaata gaggcgaagt ccattgccag gtcagcgagg
    11041 aacggcgctt ccgggcgagt cagcacaaac tgaacggtgt tgtcgtccac ctttttcact
    11101 tcgctgatca gctccggcaa gcccatgcct tcgaagtatt cgtagctgcc gccagaaact
    11161 ttatggtacg ggttttgcgc gtttttctga cgatcgaacg agaacaccac gtcatcggca
    11221 ttcatttcgc gcgtcggttt gaattcttta ttgtcgtgcc acttcacacc tttacgcaga
    11281 tggaaggtat aggttttacc gtcttcgctg acttcccact tttcagcgag gcccggaatc
    11341 acttcggtgg tgccgatttt aaattcaact aggcggttat aaagcggtac ggaagaggcg
    11401 tcataggtgg taccggaggt aaacagctgc gggttaaacc cttccggaga tccttctgag
    11461 caataaacca gagttttagc ctgaacactt gctgcgacgg tcatagccac caggctgaga
    11521 ccaagcttca gcatccctga ctttttcaag gaaatacgca ttattctgct ccaattgtga
    11581 tgtttgttgt tttaaccctt tgcagtgggt tgtcgctgcc tgaccttttt tgttttttgc
    11641 ccggtcgggt caacgttatg aggtggggat gccgtactaa ttaacatcag tgtaacagtg
    11701 cggatcctcc ataaaatgcc cctcgtgacc tacaatctgt caacagaatg tgaaaacgtc
    11761 aatacagcca accgggattt acaccaacgg tgagaatcca caaacaaaga ttaaaaaaac
    11821 ttcaaacagc tatttgcagc agagaaattt gtgctactcc aacatgacca gaacaatcag
    11881 cttaatattt agcaacattt ggtgattaag ttttatgcat ttagtgaaaa aaatctgagg
    11941 aaaaggtgga tatctgagcg attaatacca tgaacgctaa aacgcacagc ggaaaatgcc
    12001 agcgccagcc ataagtaacg caaaaaaaga tttaagcaaa tataaaaaaa gacaatggtt
    12061 tctgtcacaa atccgttaat ggatagtgag atatggggcg caaatttggc agggaaatgt
    12121 gtggcgtgta ggtcgaataa ggcgttcacg ccgcatccga caataagtgc ggtgccgttg
    12181 cctgatgcga cgctgacgcg tcttatcagg cctacgtcga gtcgaacctc cctgcccccg
    12241 gaggttctct tcctcatggg ctgcagatgc aaaaaagcct gctcgttgag caggcttttc
    12301 gaatttggtc ggtgatagag gattcgaacc tccgacccct tcgtcccgaa cgaagtgcgc
    12361 taccaggctg cgccaatcac cgaatgcggg gcgcatctta ctgcgcagat acgccctcgt
    12421 caatccctta atagcaaaat gccttttgat cggcgagaaa gtcagcaggc ctcttagtta
    12481 gccgctgcct cttttgtctg cgggatgtga caccagttgt tgttttcgtt aattccacca
    12541 tccggtgaag tatagccaag acaacccatg atggtgtcgt acagctctac gtgacggcgt
    12601 ggcactttca tgtcggctgc ttttttcagc tgcgcaaacg cctgcgcatt gaccggattt
    12661 tccagatatt tatctgacat ccagaccatc atcggtacgc ggaactgctc cggcggtgcc
    12721 agttcacgcg gcgtgccgtg caggtgctcg cgttcattaa ttgactcgcc gtggtcagct
    12781 gcgtagaaca caatcgcttt cttatcgcga acctgatcaa tcacactgga gataaagtga
    12841 tccacatagg tcaccgagtt gtcataggag ttgatcatct gcgctttggt acatccgcta
    12901 tcaacaccaa tacattccgg cttccactgc gcaaagctgc gcgggtaacg ctgggtgtag
    12961 ttaaagtgcg aacctttggt atgcagaatg attagatgct taccatccgg gttgcgcccc
    13021 agcgattgct gcatttcgtc taccagcaac atatcatcta ccggcttgcc acggttacgt
    13081 ggctccgcac caatctgttc acggtaagca atattgtccg ccatcgtatt gctgtagaac
    13141 cacatttcgc tctgcatagc gtagaggtca gaactgaatc ctaactgctt cagtaccgcg
    13201 aaaatgttct gttcttttaa tgtgcgctgc ggattatctt ccgcgccccc ctgacgtaca
    13261 aacatgcaac gcagagagag tttggttgcg gtatcacagg agtaaccacg gaacgccgcc
    13321 agatttttct cctgggccag cttcggcgtg gtattacgct catagccgaa aatgcccata
    13381 tggtcccaac gcgtggtttc accgatgata aacacgacat aagtatcatt aacattttgc
    13441 ggtgcctgat aagtgaattt cttcgccggg ttaagcaatg aattattatc ggaagattca
    13501 tcgacccgcg cccaggcgta cagccccaac gcagaaagcc agtttgatgg cagataagag
    13561 ttcgctacga caccgccata actcggcaaa tcaacgccgg tcgccctctc cactttcttc
    13621 tgctggatat ccagcaaacg aatcggtgcc caaaccataa ttcccgccag tacgacgacc
    13681 gccaggctgc gaatacgctg ccctggggtt cgcagttgtc ggagcaaggt gtagcgacag
    13741 cggttattcc agataaggat aagaggcaat gcgctaacgg cgattaacca gagaataaag
    13801 ttcagaccaa caacttcttt tgacaggtcg atatcggtgg tcatgacgga agcgatgatg
    13861 ccataaccaa tgaccacatt aaggaaggtc atgtaatagc tggcacctgc ggaaaagagc
    13921 acaaccagcg atgccagaat acgccagctg cggcggccaa acagcgaaag aagacgtagt
    13981 aaaaagaagg tcaccagtac ggtggcggcc agttcaacaa cagcagaaat gcctttccag
    14041 acggtaaaat cgtgcgcata gctgccgaag cggcggtaaa aaaccgcgcc attcataaaa
    14101 aggccaatat agattgcaag caaaaagctc agcttctgct gtgtaatcga tttgatgtat
    14161 ctcatgcaaa caaaccctgg acaaacaggt gataacaccc tgcttagcgg cttttcaggc
    14221 ccagcagggg aaaagtgcgg aggtagtcag ataagtaaaa tctacacgcg cgaataagta
    14281 gaccacagca aagggaaaaa gtgtggggaa agagtgtgca tgaagcacaa atttacaaaa
    14341 gtgcaagtcc ggaatagacc attttaacgg attaacgact attcagagat aggttgggcc
    14401 aggatgctgg cccagtaact tactcatagg tgatggaaaa atttacaagg gcattcgctg
    14461 tgccaccagt ggcctgagtg ttgacgacca cataactcgc ctggtagtga agcgacgtgg
    14521 tttcgccatc aatcgcccat tcaatcggcg ttccgctgga gcctttggga acttgctgcg
    14581 ttcctttatc gtcctctatc ttcaacgcaa cacccgttgc gccaccagcc tcaagggcaa
    14641 acatggaatc atcatcgctg tccgactgac tgtccatctc aacggtcgct ttggatatcc
    14701 catcgcactc tttgagttca atggtgaaat ctttcttgct caacaccgta ccgactgccg
    14761 caggtttttg cagcgtcatt gttcccatat cgacttcgag atcgcttttt tccatctcgc
    14821 aagctttagt ggtgatagtg atggagaaat gaatatcccc tgccgggcca agatccgtcg
    14881 ccgcattcac cccgcaggca accagactca aactggcgat gccgctatgc agcaatcgtt
    14941 tgaacttcat ggcatcactc catttcaaga tatatggtcg ttacgccttc aaacgtcccg
    15001 cctttaggcg ctttacccgt cgtgctaatc ggtaaagcca gcagtcgaat cgcggcattg
    15061 cggtgagttt tagaatccag gctgctcaaa tttaataacg tggtgctgct tgaatcattc
    15121 ggtaccaaaa ttttgccgct ggaatctttc accagtacgc caatatcggg gttgcccata
    15181 tccaccgctc ggggatcgtc agcattggtt gccccctcaa ggcgcaacgc aaccttgata
    15241 ccatcagaga tattgttgca atcgaagctg aggttaattt cttgctctgt cgcaccttca
    15301 ggcatttgcc cttgcctgtt tttaaacgcc gaggagggga attcaccaaa tggcacatcc
    15361 agcaccgtgc cgggtttaat ttcgcaatcc tgggttacgg tgacacgccc ctgaatagat
    15421 acggaagata tgggtattgt tccgtaatct ttatttgttt tcgaggcata cagttcggca
    15481 attaacgtca gagggatgac ggaatcgccg ataaagggtt tagtaatagc aaaggaaagt
    15541 agacctgcgc cgcccatata gggcgtagta atgactttgt tgcatccggt tgtttgcagc
    15601 gcctgctggt ttttgaaagg aacaaaccca tacacactgg tactgatgcg aatgttcgtc
    15661 gcaacctcca gttcctccgt caaagtataa tatttcatac tgtcaaccgt acggccagaa
    15721 gggggaagca tactcacccc ctgcactaga gtatcttttt cattcttata agaatcagga
    15781 cattcacaaa ttagcgatac gttttgtcca ttagaccagt taaggacatc cggtaaaacc
    15841 gttcctgcga cgttttttgc cgcatcaggt ataccaacat ccacatcgac attatagtga
    15901 taagtcgtgc catccgcggg ggtacatggc ccccacgcat ctccggcaaa tgccttgcca
    15961 gaaaatccga atacgcatcc taataaggat aatgctattg ccgctttcat ttcgctctcc
    16021 ttgcttactg gcaattggcc gaaacaggaa taattccgcc ctgttttaat tccagggata
    16081 atttataagg cgccacgcat tgctgcgacg cgtcattacc ccatttaatc aatacccgtc
    16141 cttcctctgg cattccgcta atatagagct gtccttcttc accaacgata ctgctcacct
    16201 cgttttgttt atcggtgatt agcgttgccg ttgctccaaa gggcaccggg ctgcccgcgg
    16261 aacgaagcag cgtcattaat acgcggtaac caacgcgggt atcaaaacgc gctctgacaa
    16321 tcgctccacg cgttggcacc actttctgga acgcattttc aacgtcaaca tcgtcaccca
    16381 acgaatcgct acgcagtgag atttcgtttt cacgatacgg catggcataa ggcacgatcg
    16441 cgttacccat ccagtcaacc tgaacgccgc cgttattcac cacgcgggca ttgcgcgcac
    16501 cgggtacgga gatgagcgtc atggtttcgc ctagcggttg ggaaagcgtc acgccttcgc
    16561 tatgaactat cacgccgccg cgaacgctgt agttgagctg ccggtaatct ttcccgtagc
    16621 tatagcctac gttgacatta ccggacccac cctgatagcc aacgttagcg ttaccgctgt
    16681 tacccacacc gttgctggcg tagccttcct ggacactgta gctcaggttg cgatcctcaa
    16741 gcagcgttcc gctgacccca acctgttgat tggtacgacc atcctggtcg gtcgtgacgc
    16801 gatagttact ccaggcccgg cctagtggaa cggaaatatt gaaagaccac aagcgatcgc
    16861 tttcatccca ttcagggctt ttattccagc tatatgcaat actgtaactg accttgccaa
    16921 tacgtccgtt gtaacccgcc gataccgtgt tctgtttacc tgcgtcgttc cagtaatcct
    16981 gctgcgttaa atttaaataa acagagccat aggcccctaa ttgctgcgtc aggttaccct
    17041 gtatttcgct gcgcttgtga taacggttat agtcgctgtc agcgtcactg cgcacatcgg
    17101 tggcttcctg gaaggtatag aaaccgctgg tagagtaccg atagcccgca aggcggaaat
    17161 cggtgccgct ttcgaagctc ttggaatata agaaacgata agattgtccc tggctatcgc
    17221 gatcgttatt cagttcgctt ttagcctgcg tcacatcaat ggaaatcgcc ccgatataac
    17281 caaagttttt ccctatccct aatgtaaatg cattgtaatt attagagatt aatacgccgc
    17341 cgtaggccgt catcccccac ggtaaaccgt agatcagatc aagctgcccg aaacgcggcg
    17401 aggcgctgtc ataattcccg gcctgatatt cacctgcggc aaagctatat ttgagatggc
    17461 cttcacgctg gaaaattggc accgatgaga atggctaaat aaagcgttgt tcactgccat
    17521 ccgcttcttt aataatgacc gtgagatcac cgctgtttgc agtggggtaa agatcgttta
    17581 tttcaaaggc accaggctgc acataggtgc gataaataac gtagccgttt tgttcaatac
    17641 tgacttcggc gttgcttttg gcgatccctc gaacgatggg tgcaaagcct cgctggctgt
    17701 cgggcagcat ctcttcatca gaggctaaca tcgcgccacg catctgaacg ctgtcaaaaa
    17761 tgtcccccgc ggtcgccgta tcccccattg ttaattgcgc tttgaaaggg atgatggccc
    17821 tactcaaaga cgtaccgata ttatcccact gcgctttgcc accggaatgg ctccaggtac
    17881 tgtaattccg taaccgccaa gcgccaaggt tcagcccgct gcgtaagttg aggtagtagc
    17941 tgtcgctctt tagcgtatct ttaccatcat catgatgaac agtgccatta tcatcgacat
    18001 aactgctgga agaccccgtc gagtcatatt cactgttgct cccgctaaaa ctgtaatcca
    18061 gcagcaacgc agggatacct tcgtcccata aagattccgg caccgtgccg cgcgccacct
    18121 ggtgcatcgc cgcctgaggg aaacttaata ccagcttttg ttcactgaag tcgaaatggc
    18181 ttgaggccag gggaatgatt tcgtcaaacg gtacgcaggc ttcggcagac accagattga
    18241 gggccggaaa gctttcaacg cgcacgccca tctctgccag ttgcgtccgg ctaaggcaag
    18301 gcacaaggga ttcccccatc gctttgcgct gtgcttctgt ggcctcttta aattccagtg
    18361 tgcgggtttc cattctctgt tcattgacgt aaacagagac acgatagatg ccgggtaact
    18421 gctgttcctg gcctttttcg aacatcgtta aatcgacatg ctggcccaca ccctgaacgt
    18481 cctccaggaa tttggggtta aactttccgg catgtgcaga aaacgtgctg gcaacagcaa
    18541 ccgccaccac agagatagaa aaggaagaaa aagtacgtga aacggtcttc cgtgacattg
    18601 agctatt
//
LOCUS       DAZFKY010000088        18573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFKY010000088 DAZFKY010000000
VERSION     DAZFKY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..260
                     /locus_tag="ACHKCL_002696"
     CDS             81..260
                     /locus_tag="ACHKCL_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763642.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            271..426
                     /gene="ydaF"
                     /locus_tag="ACHKCL_002697"
     CDS             271..426
                     /gene="ydaF"
                     /locus_tag="ACHKCL_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HFO5763643.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(423..911)
                     /gene="sieB"
                     /locus_tag="ACHKCL_002698"
     CDS             complement(423..911)
                     /gene="sieB"
                     /locus_tag="ACHKCL_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFO5763644.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            1353..1574
                     /gene="kilR"
                     /locus_tag="ACHKCL_002699"
     CDS             1353..1574
                     /gene="kilR"
                     /locus_tag="ACHKCL_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO5763645.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1574..1744
                     /gene="ydaE"
                     /locus_tag="ACHKCL_002700"
     CDS             1574..1744
                     /gene="ydaE"
                     /locus_tag="ACHKCL_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO5763646.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            1819..2094
                     /locus_tag="ACHKCL_002701"
     CDS             1819..2094
                     /locus_tag="ACHKCL_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763647.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            2196..4796
                     /gene="recE"
                     /locus_tag="ACHKCL_002702"
     CDS             2196..4796
                     /gene="recE"
                     /locus_tag="ACHKCL_002702"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO5763648.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4789..5598
                     /gene="recT"
                     /locus_tag="ACHKCL_002703"
     CDS             4789..5598
                     /gene="recT"
                     /locus_tag="ACHKCL_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO5763649.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5654..5803
                     /gene="ralR"
                     /locus_tag="ACHKCL_002704"
     CDS             5654..5803
                     /gene="ralR"
                     /locus_tag="ACHKCL_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO5763650.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            5841..6029
                     /locus_tag="ACHKCL_002705"
     CDS             5841..6029
                     /locus_tag="ACHKCL_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5763651.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            6129..6344
                     /gene="xisR"
                     /locus_tag="ACHKCL_002706"
     CDS             6129..6344
                     /gene="xisR"
                     /locus_tag="ACHKCL_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO5763652.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6346..7581
                     /gene="intR"
                     /locus_tag="ACHKCL_002707"
     CDS             6346..7581
                     /gene="intR"
                     /locus_tag="ACHKCL_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5763653.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            7633..8568
                     /gene="ttcA"
                     /locus_tag="ACHKCL_002708"
     CDS             7633..8568
                     /gene="ttcA"
                     /locus_tag="ACHKCL_002708"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5763654.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(8697..10070)
                     /gene="dbpA"
                     /locus_tag="ACHKCL_002709"
     CDS             complement(8697..10070)
                     /gene="dbpA"
                     /locus_tag="ACHKCL_002709"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5763655.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10103..10273)
                     /gene="ynaL"
                     /locus_tag="ACHKCL_002710"
     CDS             complement(10103..10273)
                     /gene="ynaL"
                     /locus_tag="ACHKCL_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5763656.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(10548..11531)
                     /gene="zntB"
                     /locus_tag="ACHKCL_002711"
     CDS             complement(10548..11531)
                     /gene="zntB"
                     /locus_tag="ACHKCL_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5763657.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11545..11592)
                     /gene="ydaN"
                     /locus_tag="ACHKCL_002712"
     CDS             complement(11545..11592)
                     /gene="ydaN"
                     /locus_tag="ACHKCL_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5763658.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            11786..13018
                     /gene="dgcM"
                     /locus_tag="ACHKCL_002713"
     CDS             11786..13018
                     /gene="dgcM"
                     /locus_tag="ACHKCL_002713"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5763659.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13039..13602)
                     /gene="smrA"
                     /locus_tag="ACHKCL_002714"
     CDS             complement(13039..13602)
                     /gene="smrA"
                     /locus_tag="ACHKCL_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5763660.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13932..14840)
                     /gene="abgR"
                     /locus_tag="ACHKCL_002715"
     CDS             complement(13932..14840)
                     /gene="abgR"
                     /locus_tag="ACHKCL_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5763661.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            15016..16326
                     /gene="abgA"
                     /locus_tag="ACHKCL_002716"
     CDS             15016..16326
                     /gene="abgA"
                     /locus_tag="ACHKCL_002716"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO5763662.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            16326..17771
                     /gene="abgB"
                     /locus_tag="ACHKCL_002717"
     CDS             16326..17771
                     /gene="abgB"
                     /locus_tag="ACHKCL_002717"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO5763663.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            17808..18573
                     /locus_tag="ACHKCL_002718"
                     /pseudo
     CDS             17808..18573
                     /locus_tag="ACHKCL_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT         5172 a         4421 c         4533 g         4447 t
ORIGIN      
        1 ttcgagttac caggcagtgg tcaggggtta agtagccagc ccgaggcgta tgaacatgac
       61 ggcgggattc aaattttgca gtgcagcagt tagttccgcc acccggcgtt aaggggatag
      121 ataagatggt gcattacgaa gtagttcagt atttgatgga ttgttgcggt atcacttaca
      181 accaggctgt gcaggcttta cgcagcaacg actgggatct ctggcaggca gaagtcgcta
      241 tacgtagcaa caagatgtga gattcgcaaa atgcaaaaaa tcgacctcgg caacaacgaa
      301 tccctggtgt gcggcgtgtt ccccaaccag gatggaacgt tcactgccat gacgtatacc
      361 aaaagcaaaa catttaaaac cgaaactggt gcgcgccgat ggttggagaa gcacacagta
      421 agctaacgat taaaacgtct actcctgctg ttccagaata acttcataaa atgggagtat
      481 ttttcggtga cgagataata agaacagttt gcgctatcac tctgatgttg aatgatgccc
      541 ttccgttcta attttttcat aaccgggtta cggcaaggag aagtgataat aagatttcct
      601 gttttaagga aatctttaaa tacagcgatt tctttctcag ataaacgaag caatactcgt
      661 tgctctggta gtaatgaata atgcttttga atatgtgctc gcaatcttga gaaggaaatg
      721 gcgaccacga aagaaaaggc aaaaacgata atctgaaaga gccaaggtat ttcagtataa
      781 gcattgaatg cgacagtaaa ctctttcggt atcagccaga gagtgagacc aaaaatgata
      841 atcgtataca taagtctttc gagtggctcg ttagcaaaaa gtttcaacaa tggagtaaat
      901 acatccaaca tatcaataac tctcaactgt aagggtattg aaatgttaac acaagctctc
      961 gctgtagggg tatagccgag accaccgaag cccggaggtg gtgaaataaa accgggcaca
     1021 acacgaaggc gcatttccga tatccataaa gagtcggtct tgtctgttaa atttaaatgg
     1081 tgggagtgcg cctccggttg taaataacga cattgctgtg tgtagtcctg gcggcatcag
     1141 tttttttctt gaagttcggc tgatgtccgc cctttttaaa gtgaattttg tgatgcgatg
     1201 aatgcggcta agcgcacgtg gcacagttaa aagtcatgtt agtccttatt ggtttgggtg
     1261 ggaaagccga ctgtaattgt taactggttg cagtcacctg gaggcaccag gcaccgcatc
     1321 aacaaagttc atttgtaaaa atggagataa ttatgattgc acatcacttc ggaactgatg
     1381 aaataccacg tcagtgtgtg actcctggcg attatgttct tcatgaaggc cggacatata
     1441 ttgcctcggc aaacaatatt aaaaagcgaa aactatatat tcgtaacctg accacaaaaa
     1501 catgcattac tgaccgcatg attaaagtct tcctcggtcg tgatggttta cctgtaaagg
     1561 cggagtcatg gtgatgacta agaaaataaa atgtgcttac cacctttgca aaaaagacgt
     1621 tgaagaaagc aaagctattg aaagaatgct tcacttcatg cacgggattt tatcaaaaga
     1681 cgaaccgaga aaatattgca gtgaagcttg tgccgaaaaa gaccagatgg cacatgaact
     1741 ttaattaatt gactattcga aactgaattt atgccagaaa tggcaggtat tcgctcaacc
     1801 ttaattaagg agaaaaacat gattaccaat tatgaagcca ctgttgtaac taccgatgac
     1861 attgttcacg aggtgaatct ggaaggaaag cgcattggct acgtaattaa aacagaaaat
     1921 aaagaaaccc cattcactgt ggttgatatc gatggtccat caggcaacgt aaaaacactt
     1981 gatgaaggtg tcaaaaaaat gtgcctggtg catatcggaa agaatctgcc cacagaaaaa
     2041 aaagccgaat ttctggcaac tctaattgca atgaaattaa aaggtgaaat ctgaaagaaa
     2101 tagcctgcgt atggcgcagg ctatgaacag tgtgtatccg gcaagatcat tcactgaaca
     2161 aaacgaattt taatctgagt tgaggttaaa aaacaatgag cacaaaacca ctcttcctgt
     2221 tacggaaagc gaaaaaatca tccggtgaac ctgacgtcgt cctgtgggca agcaacgatt
     2281 ttgaatcgac ctgtgccact ctggactacc tgatcgttaa gtcaggtaaa aaactgagca
     2341 gctattttaa agctgttgcc acgaattttc ctgtcgttaa tgacctgccc gctgaaggtg
     2401 agatcgattt tacctggagt gaacgctatc aactcagcaa agactccatg acatgggaac
     2461 taaaaccggg agcagcacca gacaacgctc actatcaagg caataccaac gtcaacggcg
     2521 aagacatgac tgagattgag gagaatatgc tactcccaat ttctggccag gaactgccca
     2581 ttcgttggct tgctcaacac ggcagcgaaa aaccggtaac gcacgtttca cgcgacggac
     2641 tccaggcatt acacattgct cgggctgaag aactaccggc tgttactgcc ctggctgttt
     2701 cccacaaaac cagcctgctc gacccgctgg aaattcgcga actccacaaa ctggttcgtg
     2761 acactgacaa agttttccct aatcctggta attcaaacct gggactgata actgcttttt
     2821 tcgaagcata cctgaacgct gactacaccg atcgaggact gctgacaaaa gagtggatga
     2881 agggtaatcg tgtttcacac atcactcgca cggcttccgg tgctaatgct ggcggcggaa
     2941 acctcaccga tcgcggcgaa ggtttcgtac acgatctgac gtcactggcg cgcgacgtag
     3001 ccactggcgt actggcccgt tcaatggatc tggacatcta taaccttcat ccggcacacg
     3061 ctaaacgcat tgaggaaatt atcgctgaaa ataaaccgcc cttttctgtt ttccgcgaca
     3121 aattcatcac catgcctggc gggctggatt attcccgcgc catcgtggtt gcgtccgtaa
     3181 aagaagcacc aattgggatc gaggtcatcc ccgcgcacgt cactgaatat ctgaacaaag
     3241 tactgactga aaccgatcat gccaaccctg atccggaaat cgtggatatt gcctgcggtc
     3301 gctcctctgc cccgatgccg cagcgagtaa cagaagaagg aaaacaggat gatgaagaaa
     3361 aaccgcaacc atctggaaca acggcagttg aacagggaga ggctgaaaca atggaaccgg
     3421 acgcaactga acatcatcag gacacgcagc cgctggatgc tcagtcacag gtaaattctg
     3481 ttgatgcgaa atatcaggaa ctgcgggcag aactccatga agcccggaaa aacattccat
     3541 caaaaaatcc tgtcgatgcc gataaattgc ttgctgcatc acgtggtgaa tttgttgacg
     3601 gaattagcga cccgaacgat ccgaaatggg taaaggggat ccagactcgc gattgtgtgt
     3661 accagaacca gccagaaacg gaaaaaacca gcccggatat gaatcaacct gagccagtag
     3721 tgcaacagga accggaaata gcctgcaatg cctgcggcca gactggcggg gataactgcc
     3781 ctgactgtgg tgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagagtc
     3841 aggttgaagc taaggaaaat gatccggagg aaatggaagg cgctgaacat ccgcacaatg
     3901 agaatgctgg cagcgatccg catcgcgatt gcagtgatga aactggcgaa gtcgcagatc
     3961 ccgtaatcgt agaagacata gagccaggta tttattacgg aatttcgaat gagaattacc
     4021 acgcgggtcc cggtgtcagt aagtctcagc tcgatgacat tgctgatact ccggcactgt
     4081 atttgtggcg taaaaatgcc cccgtggaca ccacaaagac aaaaacgctc gatttaggaa
     4141 ccgctttcca ctgccgggta cttgagccgg aagaattcag taaccgcttt atcgtagcac
     4201 ctgaatttaa ccgccggaca aactccggaa aagaagaaga gaaagcgttt ctgagggaat
     4261 gcgcaagcac aggaaaaacg gttatcactg ccgaagaagg ccggaaaatt gaactcatgt
     4321 atcagagcgt tatggctttg ccgctggggc aatggcttgt tgaaagcgcc ggacacgctg
     4381 aatcatcaat ttactgggaa gatcctgaaa cagcaatttt gtgtcggtgc cgtccggaca
     4441 aaattatccc tgaatttcac tggatcatgg acgtgaaaac tacggcggat attcaacgat
     4501 tcaaaaccgc ttattacgac taccgctatc acgttcagga tgcattctac agtgacggtt
     4561 atgaagcaca gtttggagtg cagccaactt tcgtttttct ggttgccagc acaactattg
     4621 aatgcggacg ttatccggtt gaaattttca tgatgggcga agaagcaaaa ctggcaggtc
     4681 agctggaata tcaccgcaat ctgcgaaccc tggctgactg cctcaatacc gatgaatggc
     4741 cagctattaa gacgttatca ctgccccgct gggctaagga atatgcaaat gactaagcaa
     4801 ccaccaatcg caaaagccga tctgcaaaaa actcagggaa accgtgcacc agcagcaatt
     4861 aaaaataacg acgtgattag ttttattaac cagccatcaa tgaaagagca actggcagca
     4921 gctcttccac gccatatgac ggctgaacgt atgatccgta tcgccaccac agaaattcgt
     4981 aaagttccgg cgttaggaaa ctgtgacact atgagttttg tcagtgcaat cgtacagtgt
     5041 tcacagctcg gacttgagcc cggtagcgcc ctcggtcatg catatttact gccttttggt
     5101 aataaaaacg aaaagagcgg taaaaaaaac gttcagctaa tcattggcta tcgaggcatg
     5161 attgatctgg ctcgccgttc aggtcaaatc gccagcttgt cagcccgtgt tgtccgtgaa
     5221 ggtgacgagt ttaatttcga atttggcctt gatgaaaagt taatacaccg cccaggagaa
     5281 aacgaagatg cccctgttac ccacgtctat gctgtcgcaa gactgaaaga cggaggtact
     5341 cagtttgaag ttatgacgcg caaacagatt gagctggtgc gcagccagag taaagctggt
     5401 aataacgggc cgtgggtaac tcactgggaa gaaatggcaa agaaaacggc tattcgtcgc
     5461 ctgttcaaat atctgcccgt atcaattgag atccagcgtg cagtatcaat ggatgaaaag
     5521 gaaccactga caatcgatcc tgcagattcc tctgtattaa ccggggaata cagtgtaatc
     5581 gataattcag aggaataatt cagcctggcg gtgtaatgca ccgccaactt gaaatatttt
     5641 tatgagaaaa attatgagat atgacaatgt taaaccatgt ccattttgtg gttgtccatc
     5701 agtaacggtg aaagccattt caggatatta ccgagcgaag tgtaacggat gcgaatcccg
     5761 aaccggttat ggtggaagtg aaaaagaagc actcgaacga tgaaataaac gaaccactgg
     5821 aaataataat ggaggtgttc atgtataaaa ttaccgccac tattgaaaag gaaggtggca
     5881 ctcctactaa ctggacaaga tattcaaaat ctaaactaac gaaatcagaa tgcgaaaaaa
     5941 tgctctcagg taaaaaagaa gcaggcgttt ccagagagca gaaagtaaaa ctgataaatt
     6001 ttaattgcga gaaacttcag tcctcgtgaa ttgcattgta ttcaaattaa aacttcatag
     6061 ctgattatta ataatcaaca tcgggcgtca atttaagtct aacattggcg cctgccagag
     6121 gtgatgcgat ggcacaagta atctttaatg aagagtggat ggttgaatac ggcctgatgc
     6181 ttcgcactgg tctgggggcc agacaaattg aagcataccg ccagaactgt tgggtggaag
     6241 gcttccactt caaacgagta tctcctttag ggaagccaga cagtaagcga gggattatct
     6301 ggtataacta tccaaagata aatcagttta tcaaagactc atgatatgtc taaattacca
     6361 acaggtgtcg agattcgagg taaatacatt cgcatctggt tcatgtttcg aggaaaacga
     6421 tgtcgggaaa cattgaaagg ctgggaggtt actaacagta acattaaaaa agccgggaat
     6481 ttaagagcgt tgatagttca tgaaatcaat tccggtgagt ttgagtattt aagacgtttt
     6541 ccccagtcca gcactggggc aaaaatggtg acaacgaggg tcataaaaac gttcggggag
     6601 ctttgtgata tctggacaaa aattaaagaa acagagttaa caacaaacac aatgaagaaa
     6661 acgaaatcac aattaaaaac actcaggata ataatttgtg agagtactcc gatatcgcat
     6721 attcgttata gcgatatctt aaactaccgg aatgaactgc tgcatggaga aacgctttac
     6781 ctggataatc caagatccaa caaaaaagga agaaccgtgc gcacagttga taactatatc
     6841 gccctgctct gttcgttgtt acgttttgcg tatcagtcgg gatttatatc aaccaaacca
     6901 tttgaaggag taaagaaatt acagagaaac agaataaagc ctgacccgtt atctaaaaca
     6961 gaattcaatg cattaatgga aagtgaaaaa ggacagagcc agaacttgtg gaaatttgcc
     7021 gtatactccg ggcttcgtca cggggaactg gctgctctgg cgtgggagga tgtggatttc
     7081 gagaagggag ttgtgaatgt cagaagaaac ctgacgatac tggatatgtt cggtccccca
     7141 aaaacaaatg cggggatccg gacggtaaca ctactgcagc ctgctcttga agcactgaag
     7201 gagcaataca aactgaccgg gcatcatcgc aaaagcgaaa tcacctttta tcatcgggag
     7261 tacggcagaa ccgaaaagca aaaactgcat tttgttttca tgcccagggt gtgtaacgga
     7321 aaacaaaaac cttattactc ggtaagcagt ttgggggcaa ggtggaatgc agcagtaaaa
     7381 cgtgctggta ttcgccgccg taatccgtac catacgcggc atacttttgc ctgctggctg
     7441 ttgacggcag gagcgaaccc ggcatttata gccagccaaa tggggcatga aactgcgcag
     7501 atggtgtatg aaatttacgg tatgtggatt gatgacatga acgacgaaca ggtagccatg
     7561 ttgaatgcgc ggttatcata gttgcaaagt ttgcccccaa tttgccccat ttagtaccag
     7621 agaactgaaa taatgcaaga aaatcaacaa attacaaaga aagaacaata caacctgaac
     7681 aaattacaaa aacgcctgcg tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt
     7741 gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta taccatgctg
     7801 gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac
     7861 ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct tgaaaagctg
     7921 ggcgttgagt acaagattgt tgaagagaac acttacggta tcgtgaaaga gaagattcca
     7981 gagggcaaaa ccacttgctc actgtgttct cggctacgtc gcggtatcct ttatcgcacc
     8041 gcaacggaac tgggggcgac gaagatcgcg ttgggccacc atcgtgacga tatcctgcaa
     8101 acgttgttct taaatatgtt ctacggcggc aagatgaaag gtatgcctcc gaaactgatg
     8161 agcgatgatg gcaaacatat cgttattcgt ccgctggcct actgccgcga gaaagatatt
     8221 cagcgatttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg cggttcacag
     8281 cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa acgttatcca
     8341 gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca tctgtgcgat
     8401 accaacctgt tcgatttcaa aggcatcacc cacggttctg aagtggttaa cgggggtgat
     8461 ctggcgtttg atcgcgaaga gatcccacta caaccggcgg gctggcagcc agaagaagat
     8521 gaaaatcagt tggatgagtt acggctgaat gtggttgaag tgaaataact cggatagcgc
     8581 ccgatgcgca aacgtatcgg gctactctaa tggaggctga ataagacgcg gtcagcgtcg
     8641 catccgacaa tcccgacgaa gatgtttact tttgcatcag gcaattcaac attttattat
     8701 tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat
     8761 gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg cacggcgatt
     8821 ttgccaatat ctgcgccatc aagcccgata tctcctgtca gtgcacctaa tacatcaccc
     8881 gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc tgcttccagc
     8941 gtcacaatgg aactattagc tggtggcgtt tgccagttaa gttttatctg caacatgtca
     9001 gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc cagaccgcta
     9061 tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc ccacgccagc
     9121 tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc aacatcagtc
     9181 gcgaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc acgatcgcgt
     9241 tgctccagat cgccgtgtaa tgacaatgca ctttgcccta cttcattcag cgcgtcgcag
     9301 acagcctggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag
     9361 cttaataacc gttgcaacag aggaattttg cctttgctgg atgtctcata aaattgttgt
     9421 tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg ccaaaggatc gcgttgcact
     9481 cgtccgctga ttgcggcgat ggcttccggc caggttgccg aaaacagaag cgtctgtcga
     9541 gatgcaggcg caaaacggat gacatcatca atggcatcgc taaatcccat atccagcatg
     9601 cggtcggcct catccatcac caaagtattc agcgcatcca gtgataccgt gcctttttgc
     9661 aggtgatcca gcaaacggcc cggcgttgcc acgataatat gcggcgcatg ttgcaacgaa
     9721 tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt atttggcaga
     9781 aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg cgtaggacac
     9841 agcactaaag cctgggtttg aaatagcgac gcatcaattt gctgtaacaa gccgaggcca
     9901 aaagccgccg ttttgccgct gccagttttc gcctgcacgc gaacatcttt tccggcaagg
     9961 atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa ctcattaagg
    10021 ttcgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggtcac aatctattct
    10081 cgtggtcatc gacgcaaagt catcaggcgc gtatcctcgc agatctacgc tcacgatgcg
    10141 acaatttaat cggttcttca tcgggtggtg ggtcaggcat gggttgcggg cgagggatcg
    10201 gatcgggcac tggaacagga tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa
    10261 taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta gacgctgata
    10321 aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc aattgtgcta
    10381 tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc gcttgacgtt
    10441 gcattcacct gcaagagaga tattgccctg aatgggtaga gagtttattg acttcgctca
    10501 aactttgcgg cgtttttgta tacagacagc cggaaaaatt gcttttgtta caaccattta
    10561 ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat acaaaaaatt
    10621 gaaaatccga attgccaccc gccgccaggg atcccaccaa ggttgacgcc aaataaccct
    10681 gtcagaaagg tactgggtaa aaagaccatt gccatcaatg acattgtata ggtacgacga
    10741 gctaaatttt cctgcatcac ctgagcgatt tcatccgcca tcacaccagt ccgtgctata
    10801 caggcgtcga tttcgtcaag gccgcgccca aggcgatcgg caatatcctg catccgacgg
    10861 cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata aacatcacgt
    10921 tgcggtgcca tatagcgacg catcacaatt aattgtttgc gcagcagagc caggaatcca
    10981 cgcggcggaa tttgctgatc aaggagatta tcttcaaggt cgataatttt atcgtgcagc
    11041 tgctcgataa attcgctgga atgatcggtc aacgcatcgc acacatccac cagccatccc
    11101 ccgcaatcgg tcggacccgt gccctcttcc agatcactca ccacatcgtc cagcgccagc
    11161 actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg catggcgacc
    11221 agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct
    11281 tcaccgagac ggctgactcg gggccgtgtg ctctcgcccg ccagcgcatc acgtacgtta
    11341 ttgggaagca gcggtgttgt cgccagccat tgggcgctat catggtgtac ataattaagg
    11401 tggagccagc agggatgcgc ttcatcaatc acatctgtat tttccagcgg tttaacgccg
    11461 cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc cgatccctta
    11521 atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc ttctggccct
    11581 gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc tgtctaaatt
    11641 gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct
    11701 atcattatca gaattatctg atcatatgac gtggcttttt tgcgatcgga tagcaacaaa
    11761 aattgataaa aataatggga tctcaatgat tacgcacaac ttcaatactc tggacttact
    11821 caccagtcct gtctggatcg tttcgccctt tgaggaacag ttaatttatg ccaacagcgc
    11881 ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta cgaaccggtc cctattccgt
    11941 ctcctcacaa aaagaactgc cgaaatacct ctccgatctg caaaaccaac acgatattat
    12001 cgaaatcctc actgttcagc gtaaagaaga ggaaacagca ttaagctgtc ggcttgtttt
    12061 gcgagagctg acagaaacag aaccggtgat tattttcgaa ggtatcgaag cgccggcaac
    12121 gctgggttta aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag gtttttatgc
    12181 gcgctttttt ctgactaact ctgcaccaat gttgttgatt gacccgtcac gagatggaca
    12241 aatcgtcgat gctaacctcg ccgcgctcaa tttctatggt tataaccatg aaacgatgtg
    12301 ccagaaacat acctgggaaa taaatatgct cgggcgtcgc gtcatgccta tcatgcatga
    12361 aatctcgcat ttacccggtg gtcataaacc tctgaatttt attcataagc tggcggatgg
    12421 ttcgactcgt catgtgcaga cctatgccgg accgattgaa atttatggcg acaagctcat
    12481 gttatgtatt gtgcatgata ttactgagca aaaacggctg gaggagcagc tggaacatgc
    12541 tgctcaccat gacgcgatga ccggattact gaatcggcga cagttttatc acattacgga
    12601 accaggccaa atgcagcacc tcgccatcgc tcaggattac agcttattgc tcatcgacac
    12661 agatcgtttt aaacacatta acgatctcta tgggcattct aaaggtgatg aggtgttatg
    12721 cgccctcgcc cgcactctcg aaagttgcgc tcgcaaaggc gatttggtgt ttcgttgggg
    12781 aggcgaagag tttgtcttat tactaccaag aaccccactg gataccgcgc tttcgctggc
    12841 tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg ggcttaccac gctttaccgt
    12901 cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc gatgaactgt ttaaacgcgt
    12961 tgatgatgct ttgtatcggg cgaaaaatga tggacgcaac cgcgtgctgg cggcataagc
    13021 cacggatgcg tctcgagatc aacgactgcg cttagcgtgg cgctcccagt tttcttgctt
    13081 tgcctgtgcc gttttacgta gtgcgacgta acacgccccg ctgccgccat gatgcggtag
    13141 cgcggtacaa tatgcctgga catcatcaaa ttcggtcaac cagcgcgcca catagctgcg
    13201 gacaatattg gcatgcgatt tatcatcccg ccctttacca tgaataatca gcacgttacg
    13261 caaaccatcc gccatggctt gttgaatgaa actgaacatc attttgcggc actcttccac
    13321 cggctggcgc aaaagattca ggctcgcctg ttgcggatat ttaccactgc gcagcttatc
    13381 cagcacccca tgttgcaacc cttcccgccg aaactccagc ggctgactta gcgggatgat
    13441 gtcgagaaat ccggtggtga ggaaattatc aagttgcagc gtgtcgatac gctgcggcgc
    13501 acgttggtta cgcgttggat gccagtggac atcggtagca cgtttcagcg gctggacatc
    13561 ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgttacatc ctcccgcaat
    13621 taagagcgcg atatgataac cagaccgggt cggtccaaca acgtattacc caaaattcca
    13681 gtaataagtt ccaaatattg ccgatatttt aagcaaaatg cttatgcatg attattcatt
    13741 cacgatatta ataatgtaac ttatattttc gtgaaatctg tcactgaaga aaattggcaa
    13801 ctaaaggtta aaaccgttat aacacagtca ccggcgcaga ggagacaatg ccggatttaa
    13861 gacgcggatg cactgctgtg tgtactgtag agtctggcgg atgtcgacag actctatttt
    13921 tttatgcagt tttaactttg cagatagccg cattctcgcc ggaattgcgt gattaatgac
    13981 gcggtcagtg gtgtctggcg actatcacgc cgctgaatca aataataggc cgctttcggt
    14041 aaaatttcgc taaccggcaa catcaccagc ccctgtccgt gcaagggatc gcagcccatt
    14101 tcttcaggca gtatgctgag aaaatcgctt tttgccacca gactgataca ggctgagaac
    14161 gtctcgcaga ctacaccgac ctgtggcgtt tgcgcctgat cgtcaagcaa ttcactcaac
    14221 tgtttgtagt agctgccgtg tggcgtcggc attgtccagc tgtaatccag taactgtttg
    14281 atcgaacggg caccaatggc ggggtgtccc gggcggcaaa agatcgcgaa ttgcttttcc
    14341 agtaatttct caaaagtaaa ttcgtggtcg tacggtccct gataataggt attgatggtg
    14401 aaatccaatt ctccctgacg caattcatta atcatcgaca ccagttgccc ttccataatg
    14461 cgtactttta cctgcggatg ctgctgatga aaacgagata ttacagctgg catcagactg
    14521 cgggaaatac tggcccccat gccgatatta atctgccctg ccagttgccc ttgtcgttgg
    14581 cgaatatcct cttgggctgc gcgcagttct tcaagaataa gactggcgtg ctgataaaaa
    14641 ctttcaccgg catcagttaa cgtcacgcct ttactacggc gaaaaaagag ttgcgccgct
    14701 aacccttctt ccagctcctg aatagattta ctcagtgccg gttgcgacat attcaacatt
    14761 cggctcgctc cgcgaatgct gccctgacga gccacttcaa caaaagcccg aatttgatga
    14821 atttttacct gaaaagccat gacgccaccg ataaccgtta tttatcagac caaagaaact
    14881 ggcatctact ttaatgcaga tgattgtgtc agggtaattt atgaacggtt aaaactgtga
    14941 aaaatcagtt agtgataagt aaaaactatc gctacgtgaa ccgggtcaca ctttttactg
    15001 atgacgggaa aggttatgga gtctttgaat caatttgtta attcgcttgc cccaaaatta
    15061 tcgcactggc gacgtgattt tcatcactat gcagagtctg gctgggtgga attccgcact
    15121 gccacccttg ttgcggaaga attgcagcag ctcggctact cactggcgct gggccgcgaa
    15181 gtagttaatg aaaatagccg gatgggacta cctgatgaac tcactctaca acgcgaattc
    15241 gagcgcgctc gtcaacaggg tgcgctagca caatggattg cggcttttga aggtggtttc
    15301 acgggtatcg tcgccaccct ggataccggt cgccccggtc cggtgatggc tttccgtgtc
    15361 gatatggacg cgctggatct cagtgaagag caggatgtca gccatcgccc ctaccgcgac
    15421 ggttttgcgt catgtaacgc cggaatgatg catgcctgtg gtcatgatgg gcataccacc
    15481 attgggcttg ggctggcgca tacccttaaa caattcgagt ccggactaca tggcgtcatc
    15541 aaactgattt ttcagcctgc agaggaaggt acgcgtggcg cgcgggcgat ggtcgatgca
    15601 ggtgtcgtag atgatgttga ttattttact gccgtgcaca ttggcactgg cgtacctgcg
    15661 ggcaccgtgg tgtgcggcag tgataatttt atggcaacca ccaaatttga cgcgcacttc
    15721 accggtaccg ccgctcacgc aggcgcaaaa ccagaagacg gttacaatgc cttgttggcg
    15781 gcagcacaag ccactcttgc actgcatgca atcgccccgc acagcgaagg agcttccaga
    15841 gtaaacgtgg gcgttatgca ggcaggaagc ggtcgtaacg ttgttcctgc ctcggcgttg
    15901 ctgaaagtgg aaacacgcgg ggccagcgac gtcattaatc aatatgtttt tgaccgtgct
    15961 cagcaagcga ttcagggcgc agcaaccatg tatggtgtcg gcgttgaaac tcgtctgatg
    16021 ggtgcagcta ccgccagttc tccttcgccg caatgggtcg catggttgca aagtcaggcg
    16081 gctcaggtcg cgggggtcaa tcaggccatt gaacgtgttg aagcgcctgc gggttccgaa
    16141 gatgccacat taatgatggc ccgcgtgcag caacatcaag ggcaagcctc ctacgtggtg
    16201 tttggcacac agctggcggc aggtcatcac aacgaaaaat tcgattttga cgagcaggtt
    16261 ctcgctattg ccgtcgaaac gctggcgcgc accgcgctca attttccctg gacgcgaggt
    16321 atctgatgca ggaaatctat cgttttatcg acgatgcgat tgaagccgat cgccaacgtt
    16381 ataccgatat tgccgatcaa atctgggatc atccagaaac acgttttgaa gagttctggt
    16441 cagcggagca tctggcttcg gcgctggaat ctgcaggctt caccgttacc cgcaacgtag
    16501 gcaatatccc aaatgccttt attgcttcgt ttggtcaagg caaaccggtt atcgccctgc
    16561 tgggagaata tgacgccctg gcaggtttaa gtcagcaagc aggttgcgcg caacctacat
    16621 ccgtgacgcc cggtgaaaat ggtcacggtt gcggacacaa tttgctggga accgccgcct
    16681 ttgccgctgc aatagccatc aagaaatggc tggaacaata tgggcaaggc ggcacagtgc
    16741 gcttttatgg ttgtcctggc gaagaaggcg gctcgggtaa aacgttcatg gtccgcgagg
    16801 gggtatttga tgatgtggat gcggcactca cctggcaccc ggaagccttt gccggtatgt
    16861 tcaatacccg tacgctggca aacattcagg catcatggcg ctttaaaggg atcgcagcac
    16921 atgccgcgaa ttcccctcat ttgggacgca gcgcccttga tgccgtaacg ttgatgacca
    16981 ctggcaccaa cttcctcaac gaacatatta ttgaaaaagc gcgcgtacac tatgccatca
    17041 caaatagtgg cgggatctcg cccaacgtgg tccaggcgca ggcagaagtg ctttatctta
    17101 tccgcgcccc cgaaatgacc gacgtgcagc atatttatga tcgggtcgcc aaaatcgccg
    17161 aaggtgcggc attgatgacc gaaaccacgg ttgaatgccg cttcgacaaa gcctgttcca
    17221 gttatctccc gaatcgcacc ttagaaaatg ccatgtacca ggccctatcc cattttggta
    17281 ccccggaatg gaactccgaa gaactggctt ttgcgaaaca aattcaggct acgcttacct
    17341 ccaacgatcg gcaaaacagt ctgaataata tcgccgcaac cggtggcgaa aacggcaagg
    17401 tttttgcact acgtcatcgt gaaacggtac tggcgaatga agtcgctcca tatgccgcca
    17461 ccgataacgt gcttgcggca tcgactgatg tcggcgacgt cagttggaaa ctgcctgttg
    17521 cccagtgttt cagcccctgt tttgccgtcg gtacacccct acatacgtgg caactggtta
    17581 gccaggggcg aacatctatt gctcataaag gaatgctgct ggcggcgaaa actatggcag
    17641 caaccacact caatctcttc attgattcag ggctattgca agaatgccaa caagagcatc
    17701 agcaagttac ggacacgcaa ccgtatcact gccctatccc gaaaaacgtg acaccgtcac
    17761 ctttaaaata acaacaacaa cgcaaacaca acaaccgagg aatgcccatg agtatgtcat
    17821 ccataccgtc gtcctcccaa tccgggaagc tctatggctg ggtcgaaaga attggtaaca
    17881 aggttcccca tcctttttgc tctttatcta tttgattatc gtactcatgg tgacgacggc
    17941 aattttgtcg gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt
    18001 gaaaaacctg ctcagtgtgg aaggattaca ctggttttta cccaatgtta ttaaaaactt
    18061 tagcggtttt gctccacttg gtgcgatcct ggcgctggtt ttaggtgccg gtctggcgga
    18121 gcgcgtcggc ttactgccag cactaatggt taaaatggca tcgcatgtta atgcccgcta
    18181 cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg atgcggcgtt
    18241 agtgatcatg ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc atccagttgc
    18301 aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt tactgattgt
    18361 cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcagctgcgt tcaatccgca
    18421 aatgcacgtc agtgtaattg ataactggta ttttatggcc agctccgtag tcgtactgac
    18481 gattgttggc ggcctgataa ccgacaaaat catcgagcca cggttaggtc aatggcaggg
    18541 aaacagcgat gagaaactgc agacattgac cga
//
LOCUS       DAZFKY010000089        18562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFKY010000089 DAZFKY010000000
VERSION     DAZFKY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHKCL_002719"
     CDS             complement(208..855)
                     /gene="ychE"
                     /locus_tag="ACHKCL_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5763664.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHKCL_002720"
     CDS             complement(886..939)
                     /gene="ychE"
                     /locus_tag="ACHKCL_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5763665.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1332..4007
                     /gene="adhE"
                     /locus_tag="ACHKCL_002721"
                     /gene_synonym="adhC"
     CDS             1332..4007
                     /gene="adhE"
                     /locus_tag="ACHKCL_002721"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5763666.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4308..4925)
                     /gene="tdk"
                     /locus_tag="ACHKCL_002722"
     CDS             complement(4308..4925)
                     /gene="tdk"
                     /locus_tag="ACHKCL_002722"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5763667.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5529..5942
                     /gene="hns"
                     /locus_tag="ACHKCL_002723"
     CDS             5529..5942
                     /gene="hns"
                     /locus_tag="ACHKCL_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5763668.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6086..6994)
                     /gene="galU"
                     /locus_tag="ACHKCL_002724"
     CDS             complement(6086..6994)
                     /gene="galU"
                     /locus_tag="ACHKCL_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5763669.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7196..8209)
                     /gene="rssB"
                     /locus_tag="ACHKCL_002725"
     CDS             complement(7196..8209)
                     /gene="rssB"
                     /locus_tag="ACHKCL_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5763670.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8301..9206)
                     /gene="rssA"
                     /locus_tag="ACHKCL_002726"
     CDS             complement(8301..9206)
                     /gene="rssA"
                     /locus_tag="ACHKCL_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5763671.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9319..9777
                     /gene="ychJ"
                     /locus_tag="ACHKCL_002727"
     CDS             9319..9777
                     /gene="ychJ"
                     /locus_tag="ACHKCL_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5763672.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9827..10669
                     /gene="purU"
                     /locus_tag="ACHKCL_002728"
     CDS             9827..10669
                     /gene="purU"
                     /locus_tag="ACHKCL_002728"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5763673.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10829..10913
                     /locus_tag="ACHKCL_002729"
     tRNA            10829..10913
                     /locus_tag="ACHKCL_002729"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10863..10865,aa:Tyr,seq:gta)
     gene            10958..11088
                     /locus_tag="ACHKCL_002730"
     ncRNA           10958..11088
                     /ncRNA_class="other"
                     /locus_tag="ACHKCL_002730"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11123..11207
                     /locus_tag="ACHKCL_002731"
     tRNA            11123..11207
                     /locus_tag="ACHKCL_002731"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11157..11159,aa:Tyr,seq:gta)
     gene            11254..11385
                     /locus_tag="ACHKCL_002732"
     ncRNA           11254..11385
                     /ncRNA_class="other"
                     /locus_tag="ACHKCL_002732"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11432..11563
                     /locus_tag="ACHKCL_002733"
     ncRNA           11432..11563
                     /ncRNA_class="other"
                     /locus_tag="ACHKCL_002733"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11747..12424)
                     /gene="narI"
                     /locus_tag="ACHKCL_002734"
     CDS             complement(11747..12424)
                     /gene="narI"
                     /locus_tag="ACHKCL_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5763674.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12424..13134)
                     /gene="narJ"
                     /locus_tag="ACHKCL_002735"
     CDS             complement(12424..13134)
                     /gene="narJ"
                     /locus_tag="ACHKCL_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5763675.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13131..14669)
                     /gene="narH"
                     /locus_tag="ACHKCL_002736"
     CDS             complement(13131..14669)
                     /gene="narH"
                     /locus_tag="ACHKCL_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5763676.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14666..18409)
                     /gene="narG"
                     /locus_tag="ACHKCL_002737"
     CDS             complement(14666..18409)
                     /gene="narG"
                     /locus_tag="ACHKCL_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5763677.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4698 a         4856 c         4447 g         4561 t
ORIGIN      
        1 caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga tgatgaggag
       61 aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt ttacctattc
      121 cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt aaacggagca
      181 tggcagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa tccccttaat
      241 accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa tacgcgtaat
      301 cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc ggaacaatcc
      361 ccaacaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat agctaatgct
      421 gtgataacgc gtaccccaga cgatggtaga actgatcgcc cctggccccg ccatcaacgg
      481 caacgccaat ggcaccacac caatactttc acgtaccgcg gtttctgatt tttcttgctt
      541 gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg tcaccaccag
      601 gatgcccccg gcgatacgga acgaatcaat tgatatacca aaaagttgca gaatcgtgtc
      661 gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg taaggttagt
      721 tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg gaataatccc
      781 taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa
      841 aagggtctga atcacggtta gctccgaagc aaaagccgga taatgttagc cataaataag
      901 gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc taactactta
      961 aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg tagccaccaa
     1021 atcatactac aatttattaa ctgttatcta taatggcgaa aagcgatgct gaaaggtgtc
     1081 agctttgcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag taatcttgct
     1141 tacgccactt ggaagtgacg cattagagat aataactcta atgtttaaac tcttttagta
     1201 aatcacagtg agtgtgtgcg cgagtaagct tttgattttc atcggttaag caaatcatca
     1261 ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt taacattatc
     1321 aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta gagcgtgtaa
     1381 aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa atcttccgcg
     1441 ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc gttgccgaat
     1501 ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct gaatatatct
     1561 acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac acttttggta
     1621 ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc actaacccga
     1681 cttcaacagc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc attatcttct
     1741 ccccgcaccc gcgtgcaaaa gatgcgacca acaaagcggc tgatatcgtt ctgcaggctg
     1801 ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct tctgttgaac
     1861 tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact ggtggtccgg
     1921 gcatggttaa agccgcatac agctccggta aaccagctat cggcgtaggc gcgggcaaca
     1981 ctccggttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct gtactgatgt
     2041 ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt gtcgttgact
     2101 ctgtttatga cgcagtacgt gaacgtttcg caacccacgg cggctatctg ttgcagggta
     2161 aagagctgaa agctgttcag gacgttatcc tgaaaaacgg tgcgctgaac gcggctatcg
     2221 ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca gaaaacacca
     2281 agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca catgaaaaac
     2341 tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta gaaaaagcag
     2401 agaaactggt tgctatgggt ggtatcggtc atacctcttg cctgtacact gaccaggata
     2461 accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt atcctgatta
     2521 acaccccggc ttctcagggt ggtatcggtg acctgtataa cttcaaactc gcaccttccc
     2581 tgactctggg ttgtggttcc tggggtggta actccatctc tgaaaacgtt ggtccgaaac
     2641 acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg cacaaacttc
     2701 cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa gtgattactg
     2761 atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat ggttatgctg
     2821 atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc ttcgaagtag
     2881 aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac tccttcaaac
     2941 cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag atcatgtggg
     3001 ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt atggatatcc
     3061 gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatt gctgtcacca
     3121 ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac gacgctactg
     3181 gtcagaaata cccgctggcg gactatgcgc tgaccccgga tatggcgatt gtcgacgcca
     3241 acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac gcagtaactc
     3301 acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt caggctctgc
     3361 aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg tctaaaaatc
     3421 cggtagcgcg tgaacgtgtt cacagtgcag cgactatcgc gggtatcgcg tttgcgaacg
     3481 ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc catattccgc
     3541 acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg aacgacaacc
     3601 cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt cgttatgctg
     3661 aaattgccga ccacctgggt ctgagcgcac cgggcgaccg tactgctgct aagatcgaga
     3721 aactgctggc atggctggaa acgctgaagg ctgaactggg tattccgaaa tctatccgtg
     3781 aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct gaagatgcat
     3841 tcgatgacca gtgcactggc gctaacccgc gttacccgct gatctccgag ctgaaacaga
     3901 ttctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca gcgaaaaaag
     3961 aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca gtagcgctgt
     4021 ctggcaacat aaacggcccc ttctggggcc gtttttttgt ttacccaaag caacttttcc
     4081 ataaaccgac agcattagcc ttcatcatat ttgcgacgat gtataacgcc taaacacagg
     4141 gatattgtac tttacaggtc acaagtcaac gtcggtgctt aagagccctg tgaggcgtat
     4201 agcggcgtta aaaactgccg agaagggtgt atagcccgga agaagtgcgt aaaacgaact
     4261 gacaggataa aagtgccctg ctcaccctgt cagtaaagaa attcttatta atcgtggcga
     4321 tgcctttcct gaatagccgt taatgagccg acttgtaacg cctctttata gtgtttacgg
     4381 catacagaaa cgtatcgttc attaccacca ataaccacct gctcaccttc gttataaggt
     4441 ctgcctgctt gatcaagacg cagcaccatg cttgctttac ggccacaaaa acagatggtt
     4501 tttaattcaa ccagtttgtc ggaccatgcc agtaagtatt ggctgccaat aaataattca
     4561 cctcgaaaat cggtacgtaa accataacaa agtacgggta tatcgagttg atcgacaacc
     4621 tccgataatt catatacttg ttgtctggtt aaaaactggc attcatcaac cagtacgcaa
     4681 tgaattgcct gctgctcatg ttccgcacga atctcatcaa ataatgatga attttggtta
     4741 aataattttg caggcgatga caaacctata cgcgaactga ctttcccggc accaaagcga
     4801 tcatcaattt ctgccgtata tacgacagtg cgcatgccgc gttcctggta attgtatgaa
     4861 gattgcaaca atgctgtaga cttacccgca ttcattgcgg aatagtagaa atatagctgt
     4921 gccatcagcc acaggccctc aatgatatga aataaagttg gctggagttt atcataattc
     4981 gtcagttttt cagctcaaca gtatgcacag aagtggtata tgcgttctcc cttacgaagc
     5041 cttgcataat ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg
     5101 actgctggta actattcaca atctttaacc tgttgcgcaa gtaatagccc tctgttgacc
     5161 tccaggagat agtgcaatac taagtccctg ctcttattgc gactgttcta cttttcatca
     5221 ttcgcttaat agggaattct cgtaaacacg actaatacag aagactgaaa ggtcgtcagc
     5281 ctacgataat ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc
     5341 gaaatcatcg gtacaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag
     5401 atatttattg gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg
     5461 cacaactgaa tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga
     5521 ttactacaat gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg
     5581 caagagaatg tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta
     5641 acgaacgtcg cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac
     5701 tgcagcaata tcgcgaaatg ctgatcgctg acggtattga cccgaacgag ctgctgaata
     5761 gccttgccgc cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata
     5821 gctacgttga cgaaaacggc gaaactaaaa cctggactgg ccagggccgt actccagctg
     5881 taatcaaaaa agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat
     5941 aatcttttgt agattgcact tgcttaaaat cccgccatcg gcgggatttt ttattgtccg
     6001 gtttaagaca atttaataag aataagctat aaaaaaacgg cgtcgattgc tcaacgccgt
     6061 ttcgtggata acaccgatac ggatgttact tcttaatgcc catctcttct tcaagccagg
     6121 ctttaaattc cgtgccaagg gtgttatgac gaataccgta ttcaacgaag gcctgcatgt
     6181 aacctaattt attaccgcag tcatggctct tccctttcat atgataggct tcaaccgttt
     6241 ctttttcgat tagcatatca attgcgtcgg tgagctgaat ttcatcacca gctcccggag
     6301 gggtttttgc cagcaacggc caaatatccg cactaagtac gtaacgaccc acaatagcga
     6361 gattagacgg cgcaacatcc gcttttggtt tttcaaccac gccaaccatc ggtacgcttt
     6421 cacccggcgc taattcaacg cctttgcaat ccacaacgcc atatgcggtc acatcagcaa
     6481 ccggttcaac catgatctgg ctatgacccg tttcatcgaa gcggcggatc atctctgcca
     6541 ggttatcctg tgacaaatcg gattcatatt catccagaat aacgtcaggc aaaataacag
     6601 ccacaggttc atcacccact accgggtgag cacacaatac cgcgtgtccc aggcctttcg
     6661 ccagcccctg acgaacttgc ataatagtta cgtgcggcgg acaaatagac tgcacttcat
     6721 caagcagttg acgttttaca cgtttttcca gcattgcttc cagttcaaaa ctggtatcaa
     6781 agtggttttc aatagagttt ttagatgagt gtgtaaccag cacaatttca gtaatgccag
     6841 ccgcaataca ttcattcacg acgtattgaa ttaatggctt atcgacaagt ggcagcatct
     6901 ctttcgggat ggctttcgtc gccggcaaca tcctggttcc taatcccgca acggggataa
     6961 cggctttttt gactttcgta ttaatggcag ccatttaaat tctcctggac tgttcgtgtt
     7021 ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc agcaccccgg aggggctaag
     7081 ttctgaaaag gatgcgagtt agcataatta agacaattac ttatacgaat ggcgacgata
     7141 gtaccaccag ccgacattag ctggtaatgc aaatttagcc cgcgttatcg tttgctcatt
     7201 ctgcagacaa catcaagcgc agtcgaccac cggttcccca tatttggcat tgccaggcat
     7261 cgcatcgctg gctcaattga ttcaaataag cgttacctaa agtgcctaac ggaacaccat
     7321 tactgatttg cacctggtgt tcgccggtat ttaacgtcgc attcagaccc gcagaaacca
     7381 gaatgagatt tttcagttcg cgatgataat agccaactaa tagcggaaac tgccccggca
     7441 gattggcctg acggagtaaa tggtttacct gcttcaataa cgcgcccaac tctggcaacc
     7501 gttgattttg gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg cgtaataaca
     7561 aggcagcaag tacgccatta tgtccagctc gggttacatc aaggcaataa aatgccagat
     7621 cgttttccga aagtgcggca atatcaagca ccaggccggg tttgtccgcg gcaaccaatt
     7681 gacggtaatt aacccggcaa tgggaaatca cctgctgaac cggcggttgt agttcctgta
     7741 ataatttcgc cgctgcggca gggttatcaa ccattgcatc ccagtcgcga aaaagccttt
     7801 cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa accatctcgc
     7861 gcaagcgatt cagatcttta actggtttca gcaaaacatc ttcaacgccc agacgtaacg
     7921 ctttggcaat atctgccata ttttcagtgg cagatatcac cagaactggg gtctggtcgc
     7981 ctctgttacg tatatgctcc agcagtttaa gcccgttcat tcgtggcatc gcgatatcac
     8041 atatcatcag gtctggagtg aaacctccca gcaactcaag ggcatccacc ccatcagccg
     8101 ccagtaccgt tgtcgctccc aatgaggaaa accacgaatc cagaagcgag cgaaatacct
     8161 gctcatcttc aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg ttctctcccc
     8221 tgactggctt tactcaatag tggcatgcta ttgccacctg cgcctgtcag aattaactta
     8281 agtgtagata aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag ttcgtccatt
     8341 ttcttttcca ctgccagctg cccggccgca atggcagcgt gcgcgcgatg gaaatcaagc
     8401 gtagatattt gcgggcaaac aggttgaatc agaatatcgg gcggatcacc tgccatgcgg
     8461 ttccttttaa ggcggttctc cagcacctgg atagaagtgg tcataatctc tgttgccgtt
     8521 ggtgctgtca ccgcccgacg tgtcgttata ctgcctaacc tttctttcag acgcgcatgc
     8581 cacggcagag aatcaccatt ctcgctattt tcttcactga cattaaagga gagcaaatct
     8641 tgttgcatca aatgagcatc gtgctgcagg tcgaccgcta tcacaatatc agcacccaat
     8701 gcacgcgtga gcgaaatagg aattgggtta acgactgccc catcaaccag ccagtagccg
     8761 ttatgcgcaa cgggtgccat tagtcctgga atactgcatg atgcgcgaat agcaagatgg
     8821 agatcgcctt cagtaaacca taattcacgc ccagtactta aattggtggc aacagccgcg
     8881 aagcgacggg aacaattttc gatctctgtt tccggcatta tttcgcggta ttgattgaag
     8941 acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag tcgtaaaaca
     9001 tcccaataac tgaaagaggt cacccaatct tccagcgcag ataatcgatc gcatgcatag
     9061 gccgcgccca ccagcgaacc aattgaacat cctgcaacga tatcaatttc aatacccact
     9121 ttttttagcg cattaataac gccaatatgc gaccaacctc tcgccgcgcc agatcccagc
     9181 gccagcccta tttttatctt tctcattatt cccgcaagat tcccctggaa tgcaatcgta
     9241 gccacatcgc tactgctcgg ttaacatagt gctactcagg cggttaatac gccgttattg
     9301 tttccaggga gattatttgt gtctcagctt tgtccctgtg gtagtgctgt cgagtatagc
     9361 ctatgttgcc acccttatgt gtctggtgaa aaggttgcac ctgatccaga acatctcatg
     9421 cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt atttaataaa gacctggcat
     9481 ccatcttgtg gagcagcggc gttacgtgcc gaattgattg ccggatttgc gcatactgaa
     9541 tggttgggat tgaccgtatt tgaacattgt tggcaggacg ccgacaatat cggatttgtc
     9601 agttttgttg ctcgatttac cgaaggtggc aaaacaggtg caataattga acgttcgcga
     9661 tttttgaaag aaaacggtca atggtactat attgacggta cacgtccgca gtttggccgc
     9721 aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg ccaataatgg
     9781 ttgacggtac ggtttagcaa acactctcaa caaggttttt ccagcaatgc attcactcca
     9841 acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac gtattaccaa
     9901 tatttgctac aagcacgagt taaatatcgt acagaacaat gaatttgttg atcaccgtac
     9961 cgggcgcttt tttatgcgca cggaactgga agggattttt aatgattcca ccctgctggc
    10021 ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg ccggtcgtcg
    10081 ccggatagtg attctggtca ctaaagaagc gcattgcctt ggcgatttgt tgatgaaagc
    10141 caattatggc ggcctggatg tcgaaatcgc ggcagtgatt ggtaaccacg atactttacg
    10201 ttctctggtt gagcgttttg atataccgtt tgagctggta agccatgaag ggttaagccg
    10261 caacgagcac gatcaaaaga tggcggatgc cattgatgct tatcaacctg actacgtggt
    10321 gctggcgaag tatatgcggg tattaacacc ggaatttgtg tcacgcttcc cgaataagat
    10381 catcaatatt caccattcct tcctgccagc gtttatcggc gcacgtcctt atcaccaggc
    10441 ctatgaacgt ggcgtgaaga ttattggcgc aaccgctcac tatgtgaatg acaatctgga
    10501 cgaaggccca atcatcatgc aggacgttat tcatgtcgat catacctaca cagctgaaga
    10561 tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta agtcgcgcgc tctacaaagt
    10621 actggcgcag cgcgtctttg tttacggtaa tcggacgatt attctttaat cgccagcaaa
    10681 aataactggt tacctttaat ccgttacgga tgaaaattac gcaaccagtt catttttctc
    10741 aacgtaacac tttacagcgg cgcgtcattt gatatgatgc gccccgcttc ccgataaggg
    10801 agcaggccag taaaaagcat taccccgtgg tggggttccc gagcggccaa agggagcaga
    10861 ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatgattca
    10921 ccacagcgat gtggtaactt cggtgagatt gtgaaagttt gcactcatcg gggaagggtg
    10981 agaaccttcg actaaggttc gactcgagcg aaagcgagag aacgttttca caggcaacgg
    11041 cccgaagggt gaggagcgaa gcgacgaata atcctttccc caccgccatc tcaagcatta
    11101 cctcaaaatt tgaattaccc ctggtggggt tcccgagcgg ccaaagggag cagactgtaa
    11161 atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag
    11221 tccctgaact ctcaagcgaa tccgtaaata aatattctgc ccaagcggag aaggatgaga
    11281 agcttcgact aaggttcgac tcgagcgcca gcgagagagc gttgccgcag gcaacgacct
    11341 gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac caccatcact ttcaaaagtc
    11401 cctgaactct caaacgaatc cgtaattaaa tattctgccc aagcggggaa ggatgagaag
    11461 cttcgaccaa ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga
    11521 agggcgaagc gcgcagcgct gagtaatcct tcccccacca ccatcgcttc ctgaaataat
    11581 caaaacctgt tactccgagc taattttgcc caatggcacc acgtttaccg gacctacaat
    11641 atcctccctt gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc
    11701 ataaaaaaac cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc
    11761 gcaccagctg gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac
    11821 gcgagaacgg gaacagcagg aataacgtca tccccagcac caggtgtaga cggaagataa
    11881 acgccacacc atcgaggtgt tgagaagcgc caccgtggaa ggtcactacc gactgcgccc
    11941 agccaaccag tttcatcatc tcgctaccgt ccatatgttg ggcggagaac ggaatggtca
    12001 acaggcccag cgcgcactgg ataacgagca gcgacaggat cagtatatcc gctccggtag
    12061 tcgttgcacg cacgcgtggg ctgaacagac gacgtttaag caaaagcacg ccgccaatca
    12121 gacacagcac gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg
    12181 gcagccaggc ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa
    12241 tccccagaat accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca
    12301 tctggctgga cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa
    12361 tcaggaagac cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt
    12421 gcattagtgc tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa
    12481 gcgacgctga tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac
    12541 ctgctcttct tcccaaacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc
    12601 aattttttcc gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc
    12661 aaacagcacg gcataacggc tttcacgctg ttgcagacgc gcactcagca atgccagaat
    12721 cggcgcgata tctttcaaac cttccacggc ttcgctttgc ggcagctgcg ccaggtactc
    12781 cagatacagc ggcagatgat ccggcaattc gcggctgttt aactgcaagc cgtgctgctc
    12841 gtactgcgcc agcaggtcca ccatcgcctg gccgcggtcg cgggattcgc cgtgcacatg
    12901 ttcaaacagc aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgcgcctg
    12961 ggcatcgagc ggatccatcg tcgttaaatc gcgcaggaaa atgcccagcg catgggcatc
    13021 ctcttttggc agatttttcg acgcggcaat cgcctcaaac atctcttgtt gatgctgcca
    13081 taaggcagca tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc
    13141 ggctccgttt tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat
    13201 ttggtatctg aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg
    13261 aaggcttccc gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta
    13321 gcaatcgcca gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc
    13381 agcgcacggg tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc
    13441 agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc
    13501 agatactgta ccggaatacg caggctttcg acgtctggca gaatgccgtt gctacccagc
    13561 tcacccgcgt ctgctgcaga ctgaatcgga gagagaggcg gcacgtacca gaccatcggc
    13621 agtgtgcgat attctggatg cagcggcagc gccagtttcc attccattgc cattttataa
    13681 accggcgact gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc
    13741 tcgatgactt tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtagagatct
    13801 ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca
    13861 agataacgga tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata
    13921 cgcggatagc agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt
    13981 ttgtacgggc atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg
    14041 acgatgccat cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc
    14101 gggttcaggc agtgttcgca caggcgcggc aaatacatca tgaaggtgtt ttcgaactgg
    14161 ctatacatcg ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca
    14221 ccgcccaggt catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta
    14281 atcagcgaac gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg
    14341 ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta
    14401 gcgaagattt tacccagcag catggcacgg ttacccatgc gcggttgcag tttgccgttg
    14461 attttacgga tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc
    14521 tggcccggct tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc
    14581 cagacgtttt tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc
    14641 accatgccga cttgtgaacg aattttcatt ttacgctctc ctgtacctga tcattgcctt
    14701 cgccatctaa ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag
    14761 aacctacggt gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat
    14821 gcgtcggttt cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa
    14881 tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg
    14941 ccggaacacg ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt
    15001 caatccagtc gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca
    15061 ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac
    15121 cccacttctg gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct
    15181 ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt
    15241 caccgaaatc acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac
    15301 gccatgggat cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt
    15361 ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga
    15421 agcggatctt ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt
    15481 cgctcagggc agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca
    15541 ggatcatctc tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt
    15601 ccgctttggt gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa
    15661 tccctttacc gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt
    15721 aagttgccgg ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca
    15781 ggtcgcattc gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag
    15841 agtcgtgctg gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca
    15901 cttcggagaa tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg
    15961 ccggatcgac cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt
    16021 cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg
    16081 acagacggaa gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc
    16141 agtccacttc ttccggcttc acgccgccct gctgccccag atctttaccc tggataccgt
    16201 gctccgtccc cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca
    16261 ggttagaacg ccagataaac aggttacgcg ggtggttttt gccgttttcc ggctgttccg
    16321 cagcgaaacg gatagaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc
    16381 cggctttttc tgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggcgcagacg
    16441 gcagccagcc catgcgttct gcacggacgt taaagtcgat cagatgtccg gtatagcggg
    16501 atttatccgc catcggcgac agcagttctt ccgcagtcac ggtttcataa cgccactggc
    16561 tggagtggtt atagaaataa gaggtgctgt tcatgtgacg cgccggacgc tgccagtcga
    16621 gggcaaacgc cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt
    16681 gcgcccagcc gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac
    16741 cacgatagtt catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg
    16801 aacgaccgtg cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc
    16861 tgcgagaaac gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat
    16921 agctggttgc acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca
    16981 gatcataaac ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg
    17041 gcagtttgtg cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc
    17101 caccaaagta cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt
    17161 gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct
    17221 cgccccagcg gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg
    17281 cgacagtttt ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc
    17341 gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca
    17401 tgtcggtgta gcgacgcaca tagtcggtga aatactggct tgggttgtcg agatggaatt
    17461 cacgcagcat tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg
    17521 ccagccacag atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt
    17581 tggtgccttt gtaacgcact tcagtaaaga agtgagcatc tggggtacgc gtctgcggca
    17641 cgtttgaacc ccaggcgatg atgtagctgg agttgtacca gtcagcagat tccggtacgt
    17701 cagtttgctc gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga
    17761 agcttaagca agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca
    17821 tcgacattgc cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg
    17881 tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaaac
    17941 caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag
    18001 cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc
    18061 gtttgcgcat catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg
    18121 caccgcgcgg gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt
    18181 cagtctgctg ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg
    18241 agccggtgca gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc
    18301 gatatccatc ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg
    18361 tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc
    18421 tgtgggagcc tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac
    18481 gctctgaaac agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta
    18541 aattcctaag ataccccttt ag
//
LOCUS       DAZFKY010000090        18319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFKY010000090 DAZFKY010000000
VERSION     DAZFKY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            82..1302
                     /locus_tag="ACHKCL_002738"
     CDS             82..1302
                     /locus_tag="ACHKCL_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5763678.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1275..1904
                     /locus_tag="ACHKCL_002739"
     CDS             1275..1904
                     /locus_tag="ACHKCL_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5763679.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1905..3065)
                     /gene="ybdL"
                     /locus_tag="ACHKCL_002740"
     CDS             complement(1905..3065)
                     /gene="ybdL"
                     /locus_tag="ACHKCL_002740"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5763680.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3174..4262
                     /gene="hcxA"
                     /locus_tag="ACHKCL_002741"
     CDS             3174..4262
                     /gene="hcxA"
                     /locus_tag="ACHKCL_002741"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5763681.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4272..4469)
                     /locus_tag="ACHKCL_002742"
     CDS             complement(4272..4469)
                     /locus_tag="ACHKCL_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5763682.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4651..6756)
                     /gene="cstA"
                     /locus_tag="ACHKCL_002743"
     CDS             complement(4651..6756)
                     /gene="cstA"
                     /locus_tag="ACHKCL_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5763683.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(6937..7350)
                     /gene="entH"
                     /locus_tag="ACHKCL_002744"
     CDS             complement(6937..7350)
                     /gene="entH"
                     /locus_tag="ACHKCL_002744"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5763684.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7353..8099)
                     /gene="entA"
                     /locus_tag="ACHKCL_002745"
     CDS             complement(7353..8099)
                     /gene="entA"
                     /locus_tag="ACHKCL_002745"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5763685.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8099..8956)
                     /gene="entB"
                     /locus_tag="ACHKCL_002746"
     CDS             complement(8099..8956)
                     /gene="entB"
                     /locus_tag="ACHKCL_002746"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5763686.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8970..10580)
                     /gene="entE"
                     /locus_tag="ACHKCL_002747"
     CDS             complement(8970..10580)
                     /gene="entE"
                     /locus_tag="ACHKCL_002747"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5763687.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10590..11765)
                     /gene="entC"
                     /locus_tag="ACHKCL_002748"
     CDS             complement(10590..11765)
                     /gene="entC"
                     /locus_tag="ACHKCL_002748"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5763688.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11954..12910
                     /gene="fepB"
                     /locus_tag="ACHKCL_002749"
     CDS             11954..12910
                     /gene="fepB"
                     /locus_tag="ACHKCL_002749"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5763689.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12914..14164)
                     /gene="entS"
                     /locus_tag="ACHKCL_002750"
     CDS             complement(12914..14164)
                     /gene="entS"
                     /locus_tag="ACHKCL_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5763690.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14275..15279
                     /gene="fepD"
                     /locus_tag="ACHKCL_002751"
     CDS             14275..15279
                     /gene="fepD"
                     /locus_tag="ACHKCL_002751"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5763691.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15276..16268
                     /gene="fepG"
                     /locus_tag="ACHKCL_002752"
     CDS             15276..16268
                     /gene="fepG"
                     /locus_tag="ACHKCL_002752"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5763692.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16265..17080
                     /gene="fepC"
                     /locus_tag="ACHKCL_002753"
     CDS             16265..17080
                     /gene="fepC"
                     /locus_tag="ACHKCL_002753"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5763693.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17077..18210)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKCL_002754"
     CDS             complement(17077..18210)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKCL_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5763694.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4314 a         5194 c         4716 g         4095 t
ORIGIN      
        1 cactctgctt ttcagtgttt actttataat taacagtctt atctcactga tcaccctgta
       61 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
      121 gaagctgcac gagagcgtat tacctggaca ctcaacaccc taccccgcgt ttgtgtctca
      181 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact cgcccgacaa
      241 atgggcaaaa aaatctgcgt tttgtttatc gactgggagg cgcaattctc ttgcaccatt
      301 aactatgttc agtccctgcg cgagttgtac accgatgtca tcgaagagtt ttactgggtt
      361 gcgctcccgc ttacgacgca aaattccctt tcacaatacc aacccgaatg gcagtgctgg
      421 gaacctgatg tcgaatgggt acgtcagccc ccgcaagatg cgataaccga tcctgacttt
      481 ttctgctttt accagccagg catgaccttc gaacaatttg tacgtgagtt tgccgaatgg
      541 ttttcacaaa aacgtccggc ggcgatgatg atcggcatcc gtgcggatga gtcctacaac
      601 cgttttgtcg ccatcgccag tttaaataaa caacgttttg ccgacgataa accctggact
      661 acggccgcac caggcggtca tagctggtac atttacccca tttacgactg gaaagtggct
      721 gatatctgga cctggtatgc aaatcatcaa agtctctgca acccactgta taacctaatg
      781 tatcaggcag gcgttcctct gcgtcatatg cgaatttgcg aaccttttgg cccggagcaa
      841 cgacaaggat tatggctcta tcacgttatc gaaccggatc gctgggctgc tatgtgcgca
      901 cgagtcagcg gggtaaaaag tggcggcatt tacgccggac atgataatca tttctatggg
      961 catcggaaaa tcctcaagcc agaacattta gactggcaag aatatgcatt attgctgctc
     1021 aatagcatgc cggaaaaaac agctgagcat taccgcaata aaatcgccat ttatttgcac
     1081 tggtatcaga aaaaaggcat cgaggttcca caaacccagc aaggggacat tggcgcgaaa
     1141 gatatcccct cctggcggcg gatatgcaaa gttttactca ataacgatta ctggtgtcgg
     1201 gcattatcat ttagtcctac gaaagcgaag aactatcagc gttataacga acggataaaa
     1261 ggaaaacgtc aggaatgggg gatactatgc aacaacgatt aacgcaggat ttaacgcaat
     1321 ttctcgccag cttgccggaa gatgatcgca tcaacgcgat taatgaaatc cgcatggcga
     1381 tccatcaggt tagccccttt cgtgaagagc cagtggattg cgttctgtgg gttaaaaaca
     1441 gccaacttat gcctaatgat tacaacccaa acaacgtggc accacccgaa aaaaaactgc
     1501 tgaaaaaatc cattgaaata gatggtttta cccaacccat tgtggtcacg catacggata
     1561 aaaatgccct ggaaatcgtg gatggttttc accgccatga aattggtaaa ggctcatcat
     1621 cattaaaatt acgcttaaaa ggttatttgc cggtgacctg tctggaaggt acgcgtaatc
     1681 aacgcatcgc cgcgactatt cgtcataatc gcgctcgtgg acgccatcaa atcaccgcca
     1741 tgtcggaaat cgtccgtgaa ctcagtcagt tgggatggga cgataataaa attggcaaag
     1801 agctgggaat ggacagcgac gaagtgctgc gcctgaagca aattaacggt ctgcaagagc
     1861 tgtttgccga ccgtcaatat tcccgcgcct ggactgtcaa ataactacag ctggcgcagt
     1921 cgttcagctg ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg aatcagttta
     1981 tgtgggaagg gatcggcgca aaacaccgat agcggaatcg ccgccacgcc gtgctcccgc
     2041 gtcagccact ggcaaaactc aacatcatcc agggtagaaa ccgcgctgta atccaccagc
     2101 aaaaagtatg tgccttcgca cggtaaaatc tccagccgac tttcatttaa ggcatttacc
     2161 agaatatcgc gcttctggcg ataaaagtcc ggtaacgcaa gataatgctc aggttctgca
     2221 cgtagcatat ccgcaattgc cagctgtgcc ggggtattca ccgaaaaggt cagatactga
     2281 tgtaccttgc gaatttcggc gctgatgggc gctggcgcaa cacaataacc cactttccag
     2341 ccggtcatat gataggtctt gccaaacgat gacaccgcca ccgcccgctc acgcagctgc
     2401 ggatgcgcca gcacactggc atggccctgt tgtgaaaagt tgatgtgctc gtagacttca
     2461 tcgctaatga caaaaatctc gtgcccggcg atcgcctgcc acaaagcggc gaaatcagcc
     2521 tgctgccaga cagttgcact gggattatgc ggagtgttga ggatcaccag tctggtgcgc
     2581 tcgcttaaca atgcggcaaa ttcctgccag tcaacgcgaa aatgcggtgg ttgcagtgcc
     2641 atacgcttca ctattccccc agaaagcgcg atggcggggg cgtaactgtc atagctggga
     2701 tcaaaacaaa tcacttcatc gccattgcgc accagtgcgg taatcgccgc gtataacgct
     2761 tccgtcgccc ctgccgttac ggtgatatcg ctatcggcat ctggttgata gccatacaaa
     2821 cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat gggcgcgtat
     2881 tggtttgccc cctgggcaac gtggtacgcc agccgctcct gtaaatagcg cggaccatca
     2941 aaatcaggaa agccttgcga caggttaatc gcctggtgtt gctgcgccag cgcgctcatc
     3001 tgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag agggttattt
     3061 gtcattttta ttgtacctga cgaagttgcg tgttgaaggc actataacac gatgctagta
     3121 tttggcaatc aagacgttta gatgtctaaa taaaacaata aggacaacac aacatgcctc
     3181 acaatcctat ccgcgtggtc gtcggcccgg ctaactactt ttcacatcca ggaagtttca
     3241 atcacctgca cgattttttc actgatgaac aactttctcg cgcggtgtgg atctacggcg
     3301 tacgcgccat tgctgcggcg caaaccaaac ttccgccagc gtttggactt ccaggggcaa
     3361 agcatatttt gtttcgtggt cattgcagcg aaagcgatgt acagcaactg gcggctgagt
     3421 ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg cgcactactc gacaccgcga
     3481 aagccctcgc ccgccgtctc ggtctgccat ttgttgccgc tccaacgatc gccgccacct
     3541 gcgccgcctg gacaccgctc tccgtctggt ataacgatgc cggacaggcg ctgcattatg
     3601 agattttcga cgacgccaat tttatggtgc tggtggaacc ggagattatc ctcaatgcgc
     3661 cgcaacaata tctgctggcg gggatcggtg atacgctggc gaaatggtat gaagcggtgg
     3721 tgctggctcc gcaaccagaa acgttgccgc taaccgtgcg actggggatc aataatgctc
     3781 aggccattcg cgacgtcttg ttaaacagta gcgaacaggc gcttgccgat cagcaaaatc
     3841 agcagttaac gcaatcattt tgcgatgtgg tggatgccat tattgctggt ggtgggatgg
     3901 tcggtggtct gggcgatcgt tttacgcgtg tagcggcggc ccatgccgta cataacggtc
     3961 tgactgtcct gccacaaacc gagaagtttc tccacggcac caaagtcgcc tacggaattc
     4021 tggtgcaaag cgccttgctg ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc
     4081 agcgttttca tctgccgact acgctggcgg agctggaagt ggatatcaat aatcaggcgg
     4141 agatcgataa agtgattgcc cacaccttgc gcccggtgga gtccattcat tacctgccag
     4201 tcacgctgac accagatacg ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct
     4261 gacgtgcaaa tttagcagca gcgcgcgccg ccttttccac cgtagcgcgc gtcctgccgc
     4321 tcacggaaaa actcttcgta ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt
     4381 tcgacatagt tgtcgtaatc aggcatacca atcatcagct tcgccgcctg acctaaatat
     4441 tttccggctt ttgccagtga atcaaacatc ttggcctccg ctaaccatgt tgctgaaaga
     4501 gtgccggatg cggcgtgaac gccttatccg gcctacaaat gctatcaacg cgctcggtca
     4561 gccccctcgc cccatcgggg agagggttag ggtgaggggg aacccacggc gccatgccaa
     4621 cattcgccaa catcccccct cactctgact ttagtgcgcg ccttttgcct gcaccacgat
     4681 ctcctcgaca ttttccggca ttggctcata cggcgtttct ttcgccgttg gcttcggctc
     4741 tttcaatgcc gcaagtgccg tcttaatcga gaacagtgcc agaaccacca cgaccaccat
     4801 aaagaagatg gttaacccgg catccagacg gttgttgaac accagttgcg ccagctgtga
     4861 ctcagtatac tgcgatggaa tattaccgct gtcgatcatt gcctggaact tattagcaat
     4921 ggccaggaag ccgactttcg catccgggct aaacgctttc tgccagcctg cggtcagggt
     4981 acaaatcagc agccaggccg ttggtaccag cgccacccag gcgtaacgtt gacgtttcat
     5041 cttgaacaac accacggcac agagcatcag cgccatccct gccagcatct ggttggcaat
     5101 accaaacagc ggccacagag tgttaatacc gcccagcgga tcgaccacac cctgatgcag
     5161 gaagtagccc cacgccagca cgcacagcgc cgttgccagc aggttagcag gcagtgaatc
     5221 ggtccgtttc aggcccggag aaaccacgcc cagcagatcc tgcaacataa agcgcgcagc
     5281 acgcgtacct gcatccaccg ccgtcagaat aaacagagct tcaaacagaa tggcgaagtg
     5341 ataccagaac gccacatcca tcatgccgcc cagcgcgcca tgcagaatgt aggccatccc
     5401 caccgccaac gtcggcgcac cgcccgcacg ggaaatgatc gactgttctc ccacttcgct
     5461 ggcaatctgg ttgagcgtat ccggagtaat cgcaaagccc cagctactca ccacttgcgc
     5521 ggcagaagcg accacatccg ccgtccctgc cggagccagc accgccatcg ggctgttcat
     5581 ggcgaagtac acgcccggat cgatgataca ggcggaaacc agtgccataa tcgccacgaa
     5641 ggattccatt aacattccac cgtagccgat aaaacaagcc tgcccttcgt tcgccagcat
     5701 cttcggcgtg gtgccggaag agatcagcgc atggaagcca gacaccgcgc cacaggcgat
     5761 ggtgataaac aggaacggga acaggttacc ggtccatacc gggccagtgc cgtcaacaaa
     5821 tttggtcaac gcaggcatgg tcagcgtcgg gcgcataatc aaaatgccta ccgccagacc
     5881 aacgatcgtc ccgattttca ggaaggtaga gaggtagtca cgcggagcca gcagcaacca
     5941 taccggcagc accgccgcca caaaaccgta acccaccagc atccaggtca gctgcacgcc
     6001 ggtaaagtca aagtacggtg cccaggtcgg actttctgcc acccagccgc cagaaataat
     6061 ggcgaaaatc aggaatacca gaccgatgac cgacacttca ccaatacgcc ccggacgcag
     6121 atagcgcagg tagatcccca tgaacagcgc cagcggaatg gtgaacgcaa cggtatacgt
     6181 tccccacggg ctatgagtca gggctttcac cacgatcatc gccagtactg caaggataat
     6241 gaccatgatc ataaagcagg ccaccagcgc gatcaccccg gcggttggcc ccatctcttc
     6301 tttgaccagc tcacccagcg aacgaccgtc acggcgcgta gaaacaaaca gcaccatgaa
     6361 atcctgcacc gcaccggcga gaaccacccc ggcgagtagc cagatcatcc ctggcaggta
     6421 acccatttgc gccgccagta ccggcccaac caacggacct gctccggcaa tggccgcaaa
     6481 atggtgaccg aacagcactt tcttgtccgt cggcacatag tccagcccgt cgttatggcg
     6541 caccgctggc gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacagacc
     6601 ataaaaacgg taagcgatca gatagataca gaccgacgcc accacaatcc acagcgcgtt
     6661 gatctgttcc ccacgattta aagcaatata tcccagagca aatgctccca ttacagagag
     6721 cactgtccag acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca
     6781 tagagatgtt acattttgtt tctataacat ctttgtacga tttaacaaca cagcaaccat
     6841 gtaaatcgga gttgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac
     6901 cgagttttcc tgcaatctca tataattctg tcgggttcat cccaaaactg ccgtacccag
     6961 ccgacaagtg cagcaacgcc gcccctgttc atcgaaaacg acgatttccc agctctgatt
     7021 ttgccgccca agatgcagcg gctggcagac gccgcgtacc ttgccttcag acaccgggcg
     7081 atgatgtgtg gcgttaagtt ccgtgcccac cacacactgc ccgtcgcggg tcatcataaa
     7141 tccggccatc gatcccagcg tttcagccag cgccgccgac gcgccgccat gcagcaggcc
     7201 aaacggctga tgagtacgga tatcaaccgg catttcggct tccagcacat catcgcccag
     7261 acgggtatac acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagttc
     7321 gtcgagcgtt aaatggcgtt tccagatcat gcttatgccc ccagcgttga gccgccatcg
     7381 accacaatat cctgtagggt gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg
     7441 ttggcgatct cttgtggacg ggcgattttc cccagcggaa tgccgagttt aaactgctcg
     7501 ccaaagccgc gaatacgctg ttcttcggcg tcatcgctca cccacagcgt gcgttgcata
     7561 tcggtatcgg tggaacccgg cgaaaccaca ttacagcgca cgccgctacc cgccagttcc
     7621 agcccgacgc tcaacgccag gcttttcagc gccgctttcg atgcgccata agcactcata
     7681 ccaatacgcg gcgtgtgtgc ggcgtcggac gccacagtga caatcgcccc gccccgctga
     7741 cggcgaaact ggttcatggt ttgctggaac aggttaaacg caccgccgac attaaccgca
     7801 aaagtctgct gccagtcctc cttactgagc tgatcggtcg cgcccatgcg caaaattccc
     7861 gccgcattga ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc
     7921 gccacctgcg cagcatcggc aacatccatt acttcggtcg caaagggata ttgctcctga
     7981 gcgaacgctt gatcaaaacc tgtaactttc gctcccgctt caacaaacgc cagcgccgtg
     8041 gcgtagccga tacctttccc cgcgccggtt acccagacat ttttaccgct gaaatccatt
     8101 atttcacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccaaca
     8161 tgacaaagtc gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca
     8221 ccgaatccag accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca
     8281 gcaacggcag gatcacctca cgcagcgccg ctttgctggc ggggataggt gctggcagta
     8341 attcttcggt catcaccacc cggccagaac gtccggccac atatttcagc gacatcaaat
     8401 gctcgtcacg gctgaaatcg gccagcgcat ccgccaccat aaacggttta atatcgcgca
     8461 taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atataccccg gtaataatca
     8521 gctggttacg tccactctct ttgagcattt gctccagcgg agaacgatga aacgcgctgt
     8581 agcgccactt caccagcacc gtgtcatcgg catctggcgt cagtcgatcc accacctttt
     8641 gctgttccgg cgaacgggtc aggcccggcc cccacatatc attcaacagc gcccgatctt
     8701 catcgctctg ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt
     8761 agtcgcgcag cgcagcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc
     8821 agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa
     8881 aggcccagtc aactttattc tgcggaatat cgtgagactc cggcagtgcg taagcctgta
     8941 attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga
     9001 cgtaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc cacacactcc
     9061 acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc
     9121 accgcgcgaa gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca
     9181 tcttccatac tgaccagtgc ggcgtagatc accgccggat ggcgcagtaa caggttttcg
     9241 atctcttcgg cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc
     9301 acggtgatgt aaccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg
     9361 gcatcaaagg cgctggcatt gtgctgcggg cttttgtaat agccacggaa ggtatacggc
     9421 ccgcgcgtca tcagtcgtcc gacttcccct tgcggcagtg gatttccttc ggcatcggca
     9481 acccatactt cgtcatccgg acacattggg taaccctggg tatggataat tttctccgcg
     9541 ctatcatcaa gacgggtata gttcaccagc ccttccgcca taccaaacac ctgctgcaac
     9601 tggcaaccaa tctcagcggg aatacgcgcc gcaagcgtgg cagaaagacg tgcgccaccg
     9661 acctgtaaca gtttcagcga ggcaagctgc gcccggcttt cgccttcggt cagcgcctgc
     9721 aaccacaggc tgactgccgg cggcaccagc gcggtaacgt taatctgatg tttttcaatc
     9781 aatgggaagc aaagcgtggc gctgggatcg gccgccagaa caaccgttcc tccggcaaga
     9841 aagacgccca gcgatcccgg cgaactcatg gcgtagttat gagccgccgg gatcgcgcac
     9901 aggtagcgtg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag
     9961 tagtcgttat gagtgcgcgg gatcagcttc ggtgtaccgg tggtgccgcc ggaaagctgg
    10021 aaatagacca cttcatcagc aggtgacggc gtagcagtaa aaccgtctgc cggatggtta
    10081 atcgcatcct gcaagttatg ctcaccgctg tcgttgagta gctgcaccac gcgaatggaa
    10141 gaatgctctg caacaaatgt gttgaggaaa tcatccccgc taaacagcgc atgttggcga
    10201 tcggcaatca gcaatgcggg ttcaatctgg ctggcatagg cgttcagttc actacgctga
    10261 tggctgaaca acgccagcac cggcgcaacg cccagtttca gcagcgcgaa aaaggtaatg
    10321 taaagttcag cgacgttacc cagttgtacc agcgcggttt caccaggttt aatgccctga
    10381 cggcgtaaac tacacgcgag gttatccgcc gcctgattca gctcccggta actcaactgt
    10441 cgctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcagtc
    10501 agtggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag
    10561 cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta
    10621 gaaagtttga cgcctgtttc gcgccactca cccaacggtg acgacgcagg cacaatcccc
    10681 gctccggcaa acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc
    10741 cattcgccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg
    10801 aacggctcca gttcagcaat aacctgggtc gcggcctgat gcgggaaacc gctcagcgcg
    10861 ggggtcggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct
    10921 tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaagga
    10981 acgtgtaact cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca
    11041 tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact
    11101 tcatccggct gacgacgtgc ggaaccggct aacggaatgg agctaaaacg ctcaccgtct
    11161 ttacgtagca gcagttccgg gctggccccc agcagaacgc caccatctgc cagcgggaca
    11221 tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca
    11281 atggcggcgt cagtggtgat atcaatcaac cgtgacaaca ccactttgtc gacctgcggc
    11341 gtggcggtaa gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg ctccggaatt
    11401 gcctggcgtt ccaccacgtt cagcgactgg ctgcgggtga aacggcgtgc ggaagcttgt
    11461 ttttcctgac gggagaacga ctgccaggat tcaggaatat acagcgacga aggctgacgt
    11521 ggatcgaaag gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg
    11581 gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc
    11641 ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag
    11701 aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta
    11761 tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg
    11821 gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac
    11881 tcggggttgc atctggttgt ggttataatg aaaatgagaa gcattaactt cattaataac
    11941 aggaggctga tttgtgagac tcgccccgct ctaccgcaat gcccttctat taacaggact
    12001 tttgttttca ggaatagccg cagttcaggc cgccgactgg ccgcgtcaga ttactgacag
    12061 ccgtggcact cataccctgg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct
    12121 gaccggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa
    12181 taaccgcgtc gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg
    12241 caaactgcaa cggctctata tcggcgaacc gagcaccgaa gccgttgccg cgcaaatgcc
    12301 ggatctgatt ttaattagcg caaccggcgg ggattcggcg ctggcgctgt atgatcagct
    12361 ttccaccatc gccccgacat taatcatcaa ttacgacgac aaaagctggc aggcgctgtt
    12421 aacgcaactt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcgcagtt
    12481 tgataagcaa ctggcggcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc
    12541 cattgtctat actgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg
    12601 gcagatgctg gaacaactcg gctttacgct ggcgaagtta cccgcagggt taaatgccag
    12661 ccaaagccag ggtaaacgcc atgacatcat tcagcttggt ggggaaaatc tggctgcagg
    12721 gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagatgccg atgctattta
    12781 tgctaatccg ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt atgcgctggg
    12841 aaccgagacg ttccgtctgg attactacag cgccatgcaa gtgctggaac gacttaaggc
    12901 gctgttttaa gcattaaccg tcagacgctg tcacctgcgg cggcgtctgg cgaaaacgtc
    12961 gcaactccac cagcaccagc aataacaaca cgccgataat caataaacca aaaccgctcg
    13021 cgcttgcgga agcaaccggt gtcatcatcg ctcccaaacc acccagcagc gccgcgccta
    13081 tggcatcgcc cgtcacgttt tgtgccgtcc acaaaccgtt aatccgccct aacatcgctt
    13141 ccggggtttg cgtttgcagc attgtgtatt gcagcaacga gctgaccgca ctcaaccagc
    13201 cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa
    13261 tggcgaggaa cgatcccagc gtggagagca gcatcaataa ccctggtcgc gcgctatgcg
    13321 ccagcttccc gctggttaac gcgccaatag ccgcgccgag cgggatcgcc gcatagagaa
    13381 aaccaatctg tgccgccgac atctgccagt tgtcagccag cgccggatac agtacccgca
    13441 ccgcactcgc catcgtcaat aaaccaccca gcagcgcaat ccctcccacc agcgggctgg
    13501 cgagtagaaa acgaaatcct gccagtaatg atttcaacgg atgctcacgc ggctgcggtg
    13561 gcggtggcag tgccggaagg cttaacaacg gtagcaaggt aataaacgtg cccgccgccg
    13621 ccagcccgta gttccaggcc acaccgccag tcgccagcag taaaccgcca atcatgggcg
    13681 aaatcaccga ccccagacgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt
    13741 cacgccctac cagtgctggt gtcgccgcca atagcgccgt aacgccaagc gatgcgaaaa
    13801 aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc agcagcgcgt
    13861 tcaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt
    13921 tgcgctcata gcgatccgcc agcacaccgc cgaccatcag gccaacaaac atcgcgccgc
    13981 cggtcagcgt caccgaaagc cccacctgcc aggtggaatg tgtcatcatc tggatctgca
    14041 ccggcaccgc gacgccgagc aaacccagag acacaattga gatgaaacga gcgaggaata
    14101 ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat
    14161 tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa
    14221 catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct
    14281 ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc
    14341 gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt agtgctggag
    14401 gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcggctgccg
    14461 cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc gttaatgcaa
    14521 accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc cggagccagc
    14581 tttgccattg tgctgggtgc ggcgctgttt ggttactctt ccgcacagga acaactggcg
    14641 atggccttcg ccggggcgct ggtggcctcg ttgattgttg cctttaccgg cagccagggc
    14701 ggtgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg
    14761 gaagggctga ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc
    14821 tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatccca
    14881 gtgctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag tttaagcctt
    14941 ggcagcgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt gattggtctg
    15001 ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggccctat tgcctttatt
    15061 ggcctgatga tgccgcatat ggcgcgttgg ctggtgggtg ccgatcatcg ctggtcgctg
    15121 cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg
    15181 attgtgcccg gtgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg
    15241 atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct cgccgattac
    15301 tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg ggattacgca
    15361 gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gctgatgggc gatgcgccgc
    15421 gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga
    15481 ttggcgcggc actgggcgtc agcggtgcga tttttcagtc gctgatgcgt aacccactcg
    15541 gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgttg gtggcgatgg
    15601 tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggtgggc ggcattatca
    15661 cttcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta
    15721 ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag
    15781 cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc aacggcctga
    15841 cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctaatt gccgccgcct
    15901 tactggtacg ccggatgcgc ttgctggaaa tgggcgatga taccgcgtgt gcgctgggcg
    15961 tcagcgtcga acgttcgcgt ctgttaatga tgctggttgc agtggtgctt accgctgcgg
    16021 caacagcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac attgcccgac
    16081 gcattagcgg caccgctcgc tgggggctaa cccaggcggc gctatgcggg gcgctgttac
    16141 tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg
    16201 tcgttaccgt cagcctcggc ggtatttacc ttatcgtttt gttaattcag gagtctcgca
    16261 aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa
    16321 atataccgtt gcggaaaatc tgactgtaga aatacctgat ggtcacttca cggcaattat
    16381 cgggccaaat ggctgcggta aatccacgtt gctgcgtacc ttaagccgcc tgatgacgcc
    16441 tgctcatggg catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaagt
    16501 tgcccggcgg attggtctgt tggcgcaaaa tgccaccacg ctgggagata tcaccgtgca
    16561 ggagctggtg gcgcgtgggc gttatccaca tcaaccgctg tttacccgtt ggcgcaaaga
    16621 agatgaagag gcggtaacga aagcgatgca ggccacggga ataactcatc ttgcagatca
    16681 aagcgtggat accctttccg gtgggcaacg ccagcgagcg tggatcgcga tggtgctggc
    16741 ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata tcagtcatca
    16801 gattgatttg ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc
    16861 ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcgaga
    16921 agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac tgattgagcg
    16981 catttatggt ctccgctgca tgatcattga cgatccggta gccggaacgc cgctcgtggt
    17041 gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt ctgccatcat
    17101 ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac aagccaccat
    17161 cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt cttttttcac
    17221 tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt tgatatttgc
    17281 ttttgttaac tgctcaacaa gatactgacg attacgtaat tcaccgttca gttccgcaac
    17341 gtcagtgacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat
    17401 agagaaatcg gggtcatctt taacggcctg accattactg taaacgggct ttttaattcc
    17461 tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt
    17521 tttcatttta atgcggtcct gagccagttt ttctttttca aactgggttt tgatctccag
    17581 tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata
    17641 cccgctcaaa acggtctgcg cctcttcact ggttggcgcg gtaaaactta gcgtccagga
    17701 ggtatacagt gacggttcat cttttttctt actggcattg tcatcaaccg ctttcatttt
    17761 ttcgctcaat gcgacaattg cgcgatgcaa atccagttcg tcgattttcg cctcttttaa
    17821 ttggtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa ccgactgaaa
    17881 cttcttgata aacaggttaa atgcttctgt acgatcaatt ttgatatcca gatccagcac
    17941 acgaagctta gtgaatgttt tctccaactc ttgccactga acaggttctg gaggcgtgac
    18001 aaccgccgcg ctggtccatt tttgcggcag gatgaaagag atcagcaagc ctgcacaggc
    18061 aaacgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactg agattagatt
    18121 aagtaaatca atttcattat tactgggcga cgccagagga taatcgggaa aatgagcgtc
    18181 acttccctgt ttaatattca gtgatgacat ggggtataac ctgaaaatga gtccaatagg
    18241 gggaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag ctgaagtttg
    18301 taaattcatt ccactgaat
//
LOCUS       DAZFKY010000091        18252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFKY010000091 DAZFKY010000000
VERSION     DAZFKY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..902)
                     /gene="trpA"
                     /locus_tag="ACHKCL_002755"
     CDS             complement(96..902)
                     /gene="trpA"
                     /locus_tag="ACHKCL_002755"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5763695.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(902..2095)
                     /gene="trpB"
                     /locus_tag="ACHKCL_002756"
     CDS             complement(902..2095)
                     /gene="trpB"
                     /locus_tag="ACHKCL_002756"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5763696.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2107..3468)
                     /gene="trpCF"
                     /locus_tag="ACHKCL_002757"
     CDS             complement(2107..3468)
                     /gene="trpCF"
                     /locus_tag="ACHKCL_002757"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5763697.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3469..5064)
                     /gene="trpD"
                     /locus_tag="ACHKCL_002758"
     CDS             complement(3469..5064)
                     /gene="trpD"
                     /locus_tag="ACHKCL_002758"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5763698.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5064..6626)
                     /gene="trpE"
                     /locus_tag="ACHKCL_002759"
     CDS             complement(5064..6626)
                     /gene="trpE"
                     /locus_tag="ACHKCL_002759"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5763699.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6648..6741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6718..6762)
                     /gene="trpL"
                     /locus_tag="ACHKCL_002760"
     CDS             complement(6718..6762)
                     /gene="trpL"
                     /locus_tag="ACHKCL_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5763700.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6900..7781
                     /gene="rnm"
                     /locus_tag="ACHKCL_002761"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6900..7781
                     /gene="rnm"
                     /locus_tag="ACHKCL_002761"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5763701.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7778..8398
                     /gene="yciO"
                     /locus_tag="ACHKCL_002762"
     CDS             7778..8398
                     /gene="yciO"
                     /locus_tag="ACHKCL_002762"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5763702.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8426..10009
                     /locus_tag="ACHKCL_002763"
     CDS             8426..10009
                     /locus_tag="ACHKCL_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5763703.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10222..11094
                     /gene="rluB"
                     /locus_tag="ACHKCL_002764"
     CDS             10222..11094
                     /gene="rluB"
                     /locus_tag="ACHKCL_002764"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5763704.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11134..11724)
                     /gene="cobO"
                     /locus_tag="ACHKCL_002765"
     CDS             complement(11134..11724)
                     /gene="cobO"
                     /locus_tag="ACHKCL_002765"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5763705.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11721..12479)
                     /gene="yciK"
                     /locus_tag="ACHKCL_002766"
     CDS             complement(11721..12479)
                     /gene="yciK"
                     /locus_tag="ACHKCL_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5763706.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12699..13748
                     /gene="sohB"
                     /locus_tag="ACHKCL_002767"
     CDS             12699..13748
                     /gene="sohB"
                     /locus_tag="ACHKCL_002767"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5763707.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13784..14035)
                     /gene="yciN"
                     /locus_tag="ACHKCL_002768"
     CDS             complement(13784..14035)
                     /gene="yciN"
                     /locus_tag="ACHKCL_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5763708.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14415..17012
                     /gene="topA"
                     /locus_tag="ACHKCL_002769"
     CDS             14415..17012
                     /gene="topA"
                     /locus_tag="ACHKCL_002769"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5763709.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17222..18196
                     /gene="cysB"
                     /locus_tag="ACHKCL_002770"
     CDS             17222..18196
                     /gene="cysB"
                     /locus_tag="ACHKCL_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5763710.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4431 a         4549 c         4840 g         4432 t
ORIGIN      
        1 aaacggtaaa aagataaata ttaaatgaat ttaggatttt tccggcttcg ttaaagaaag
       61 ttaaaatgcc gccagcggaa ctggcggctg tgggattaac tgcgcgtcgc cgctttcatc
      121 ggttgtacaa aagctttcag tgccgccagc attttctttg gctcattaat atgttgctcg
      181 atgattttaa caatcgcaga accagaaatc gcgcccgcag ctcctgcatc aatcgctgct
      241 tttacctgat ccggggcgga aataccaaat ccctgcaatg gaggtgccgc gttgtactct
      301 ttcagcttcg caaccagatg attgaggggt aacgcggcgc ggttttctgc gccggtcacg
      361 cccgctcgtg acagcaaata tgtgtaacca cgaccgtaag aggctatctg gcgcagcagg
      421 tcgtcatcgg catttggcgg gcagatgaag ataggtgcga cattatgacg caacgcggcc
      481 tggcggaagg gcgcggactc ttcaactggc acatcggcaa ccagcaccga atcgacgccg
      541 actttctcgc actgggcata aaactcatca atgcctttgt taaacaccag gttggcatac
      601 atcagcaggc caatgggaat ggtcgggtgt ttctggcgaa tgagtgccag catctcaaag
      661 cactgcgccg gggtcacacc tgccgcaaag gcacgcagtg tggcgttttg aatcgtcggg
      721 ccatctgcca gtgggtcgga gaaggggatg cctaactcca gcgtgtcagc accggcttca
      781 attagcgtat cgataatttt cagcgactgc tcaatgcccg gatcaccgag cgtgacgaaa
      841 ggaacgaatg cgccttcttt gcgctccttc aactgggcaa acagagattc gtagcgttcc
      901 atcagatttc ccctcgtgct ttcaaaatat cgtgaacggt gaagatgtct ttatcgccgc
      961 gaccggaaag gttaaccacc agtagctgct ctttttccgg gttttcgcgc atcattttca
     1021 gcgcatgggc cagggcgtgg gaggattcca gcgccgggat aatcccttcg tgcaggcaca
     1081 gcgttttgaa ggcttccagt gcttcatcat cggtaattga cacataatca gcgcgtccag
     1141 tgctgttaag atacgcgtgt tgtgggccga cggacggaaa atcgagtccg gcggagatgg
     1201 agtaagattc ttcaatctgc ccgtcttcgg tttgcatcat cggcgctttc ataccgaaat
     1261 agatacccac gcgaccatgt tttagcggtg cgccgtgctc gccagtttcg ataccgtgac
     1321 caccaggctc cacaccaatc aggccgacgt tggtttcgtt gatgaaatct gcaaacatgc
     1381 cgatggcatt cgaaccgccg ccaacacagg cgataacggc atccggcagg cgaccttctc
     1441 tttccagaat ctgtgctttg gtttcttcgc caatcatccg ctgaaactca cggacaatgg
     1501 tcggataagg atgtgggcca gctgcggtgc ccagcatata gtgcgcggtt tcataactgc
     1561 cggaccagtc gcgcagcgcc tcgttacagg catctttcag cgtcgcggaa ccgctatgca
     1621 ccgggatcac ttccgcaccc attaaacgca tacgaaaaac gttaggcgac tggcgttcaa
     1681 cgtctttggc ccccatataa atacggcatt tcaggccgag cagggcgctg gcaagggccg
     1741 acgccacgcc atgctgaccg gccccggttt cggcgatgat ttcggtttta cccatccgct
     1801 tcgccagtaa agcctgaccg agcacctggt tagttttatg cgcgccgccg tgcagcaaat
     1861 cttcgcgctt cagatacagc gtggtgttcg tgccagccgt aatgttctgg catttggtca
     1921 gcgcggttgg acgcccggca tagtttttca gcaggtcgtt gaactgagcc tgaaattcag
     1981 gatctttttg cgcgctgaca aaagcttctt ccagctggcg cagagcaggc atcaggattt
     2041 gtggcacgta catgccgcca aactcaccaa aataggggtt aagtaatgtt gtcattgttc
     2101 ctttccttaa tatgcgcgca gcgtctggaa aaccgaggcc aaaagacgtg cgtctttgat
     2161 gcccggttgc gactctacag cagaattaaa atcaagtccg gcgcagccgg tttgtgccgc
     2221 ttccacgcag ttatctgcgc ctaagccccc cgccagcaga acgttgccaa gcgattgacc
     2281 atttaatagt gaccagtcga aacgttgccc gcttccaccc tggccgttgt ctaaaacata
     2341 tttatcaacg tgctgaagct cgcgggcggg cagggtttca ccgacgctta atgctttcca
     2401 gatggcaacg tgtgctggca gagcttcacg cagcgtatcg atatacagct gatcttcatt
     2461 accatgcagt tgcactgcca ccagtgataa caccttagct ttgtccagca catcggcaat
     2521 atcgtgattg cggaacacgc caacatactg caacggtgcc gcagccatca cttcctgcgc
     2581 ctgttcaacg ttgacgcaac gcggtgatgt cgcaacaaaa atcaacccac cgtaaatcgc
     2641 gcccgcgtcg tacgctgctt tagcatcttg cccacgcgtc aggccacata ctttattctc
     2701 acccagcaac acccgacgca cggcggcgtg caaatcgtca tgggccatca acgccgaacc
     2761 aattagaaaa ccgtcagcga agtggcttaa ctcgcgcacc tgagcgtaag tattgatgcc
     2821 tgattcgctg attaccgtca cgttgtgccc cagtttcggc gcaagctcgc gggtacggtt
     2881 gagatcaatc gacaaatcac gcagatcgcg gttgttgatg ccaacgacct ttgcccccaa
     2941 tgcaatggcg cgctccagtt cctcttcatt actgacttcg gtcagcacac ccatcttcag
     3001 actgtgggcg acggcggcaa gctggcgata ttgttcgtca tccagtactg aaagcattaa
     3061 taagcaggca tcggcctggt aatagcgcgc cagatagatc tggtaagggt cgataatgaa
     3121 gtctttacat aaaatcggct gcggggcgat ttggctgacg atggggagga aatcaaagct
     3181 cccctgaaaa tatttctcat cagtcagcac cgaaattgcc gaagcgtaat gtttataaat
     3241 ggcggcaatg cgtgctggat cgaaatcatc acagatcacg ccttttgacg gcgacgcttt
     3301 cttgcactcc agaataaacg ccgtgcgcgc accctgtagc gcatcataaa aatgtcgcgt
     3361 gctcggctga acctcattct gaaaactggc cagcggttgc tgctgtttgc gggcttctac
     3421 ccaaatcgcc ttgtctgcga cgattttcgc taaaacggtt tgcatcattt accctcgtgc
     3481 cgccagtgcg gtgactctgt cgtaagcgga accactgcgc agtacctcaa gaacggtttg
     3541 cgcattggct tgcagatctt catggccatg caggcgcatt aacatggcga cattcgccgc
     3601 gacggctgct tcatgggcgg cgtcgccttt accttgtaac aagcgtgtta aaatgtcacg
     3661 gttttcttcc ggtgttccgc ctgccagttg ctcctggtgg tagggagtca ggccaaaatc
     3721 ttcagcggtc aattgatagc tcttaatttc gccgtcatgc agctcggcaa cgattgtcgg
     3781 cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc accgccgcgc gttgataccc
     3841 cagcacgcgt aaggtttcgg caatcggcag caccagttcc ggactataaa caccaattaa
     3901 cgccagcggc ggatgcgccg ggttaatcaa tggccccagc acattgaaca gggtgcgggt
     3961 ttttagttgc tggcgaaccg gcattgcatg gcgaaatccg gtgtgatatt tcggtgcaaa
     4021 gaggaaacat acacctaact catccagcgc ctggcgcgat ttatcggcgt tcatatcaag
     4081 attaataccg aacgccgcca gcagatccga cgagccagat ttactggaga cgctacggtt
     4141 gccgtgtttc gccactttca gcccacaggc cgcggcgaca aacgcactgg cggtggaaat
     4201 attgatgctg ttgctgccgt caccgccggt gccgacgata tcggcaaaca gataatccgg
     4261 acgcgggaac ggcgcggcgt tttccagtag cgcggttgct gctccggcga tctcgttcgg
     4321 gtgctcgccg cgaattttca tgctcaccag cgccgccgcc aggtgttccg gtttcagttc
     4381 accacgtacc accgctgaaa atagctgatg actttcctgc tggctaaggg tctgcgcctg
     4441 atacagtttt tccagaatcg gttgcagcgt gttggttggc tctagtttct gctgcgccca
     4501 ggccagcgtt tgttccagca ggcgagcgcc ctgggtggtg agaatggatt ccgggtggaa
     4561 ctggaatccg caaacgcgat ctgcatcgtg acgcaccgcc atcaccatgc cattaaaatg
     4621 ggcgttgatg gttaaaccgg ccggaatatt actgccaacc agcgagtgat aacgcgccac
     4681 cggcagcggg tttgttaatc cggcaaacat cgcctgaccg tcatgttcaa tgctcgacgc
     4741 tttaccgtga agaatttcgc ccgcctgacc gacatagccc ccgtaagctt cgacaatcgc
     4801 ctgatgacca aggcaaatgc caataatagg cagcttaccg cgcaggcggg taagcagttc
     4861 aggcatacaa ccagcttcgc tcggcacacc gggtccagga gaaagcatca gcaccggatt
     4921 gctcatcgtc gcaaggcgtt caattaaggt ctgcgccgga atatggttgc ggtaaatcac
     4981 cacgttatga ccattgctgc gcaactgatc tgccaggttg taagtaaaag agtcgatatt
     5041 atcgagcagc agaatgtcag ccattagaac gtctcctgtg catgatgcgc ggtggcaata
     5101 gcgcgcagta cagcgcgggc tttattacgg gtttcgtcgg cttccgactg cggaacagaa
     5161 tcaaggacta cgccagcacc ggcttgcacg gtagcgatac cgttttccac cagcgccgag
     5221 cggatcacaa tgcaggtgtc gagatcgccg tgcgcggtga aataacctac cgcgccgccg
     5281 tagctgccgc ggcgacgacc ttcagcctcg gcaattaact gcatagcgcg tactttcggc
     5341 gcaccgctta acgtccccat attcatacag gcgcggtaag cgtgcagggc gtcgagatcg
     5401 tggcgcagct caccgaccac gcgggagacc aggtgcatca cgtaagagta acggtcaact
     5461 ttggtgaggt cggcgacgta gcggctgccg ggggtgcaaa tgcgtgccag gtcattacgg
     5521 gcgagatcca ccaacatcag atgttcagaa agctctttat gatcggtacg catttccagt
     5581 tcaatacggc tttcaaggtc cctgtcgagc gagccatcgg cgcgacgacc gcgtggacgt
     5641 gttccggcaa tcgggtagat ctcaatctgg cggctggtgg cgtcatactt gagcgaactt
     5701 tccggcgacg cgccaaacag ggtgaaatca ttatcctgca taaaaaacat gtacgggctg
     5761 ggattactct ttttcagcac gtaataggcc gccagcggtg acgggcaggg cagagagaaa
     5821 cggcgagatg gcaccacctg gaaaatttct ccggcgcgaa tcgctttttg caacaaacgc
     5881 actacgccac cgaactcttc atcgctctgg ttacattcac aacgcatatg cggcacggaa
     5941 accaccggca gcggtggcgc ggtttcggtc agttgctggc gaagatcgtt caggcgagca
     6001 gtgagacgtt gtttttcttc ttcattcgga gcaaacaggc tggcctgaat gcgggtgctt
     6061 tttttctgat ggtcaatcac catcagagtt tcagcgagat aaaaacagaa atcagggcag
     6121 ttattttccg ctgacagttg cggtaaatct tcaaaccccg ccacaaggtc ataagagaac
     6181 aggccgccga agaacatggc ttctcgttct tccttcggta cattcaacag attctgcaat
     6241 aagcggaaag cgtcaaaaac cgaaagggag cataagcgag cgtcttcatc cagcagtgga
     6301 ccaacagggg ggaagcgcag cacacggcag tttggtaatt gttcattttc cacacccgca
     6361 ggcagggcgt tatccagtag tgtcagcagg gcttcgccgt tgccggaaag ggactggatt
     6421 gtgacagtgt cacctaaagc tgtaatgcgc agcgcactgt ctaccagcag caggcttttt
     6481 aaatcatctt tgctgtcgat atctgcggat tccagcagca gcgttgccgg acgatcccca
     6541 cacaattggt gaaaaagcgc ggtgggattg tcgcgataag cgccttcgca ggttagcagt
     6601 tcgagagtcg gtttttgtgt ttgcattgtt attctctaat tttgttcaaa aaaaagcccg
     6661 ctcattaggc gggctgggta tctgattgct ttacgcatgg tgaatacact gcccgtttca
     6721 ggaagtgcgc caccaacctt tcagtacgaa aattgctttc attgtcgata ccctttttac
     6781 gtgaacttgc gtactagtta actagttcga tgattgattg tcaacagctc atttcagaat
     6841 atttgccaga accgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcacct
     6901 tgagcgacac gaattacgca gtgatttacg acctgcacag ccataccaca gcttccgatg
     6961 gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg
     7021 cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa atttcacgtt
     7081 cgggactggc gctgaatctt attcccggcg tggaaatttc cacggtctgg gaaaatcatg
     7141 aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt gagttcctcg
     7201 cgcagcagac agaacggcgc aatcagcggg cgcagcaaat tgccgaaaga cttgaaaaag
     7261 cgcatatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc gcagtgacgc
     7321 gcggtcattt tgcgcgtttc cttgttgagt gcggcaaagc cagttcaatg gcggatgtct
     7381 ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg tgtacaatag
     7441 aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct catcctgggc
     7501 ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc gaacacgacg
     7561 gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgtacc cagctggcga
     7621 cgcttgcccg ccagcaccat ttatgggcat cacaaggatc tgattttcat cagccatgcc
     7681 cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc gtctggcagc
     7741 tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt tttatattca
     7801 tcctgataac ccgcagcaac gtctaatcaa ccaggcggtg gagatcgtgc gtaaaggcgg
     7861 ggtgattgtt tatccaactg attccggcta tgcgctcggc tgtaaaattg aagataaaaa
     7921 cgccatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact ttaccctgat
     7981 gtgtcgcgat ctttctgaac tctcgaccta ttcatttgtc gataacgttg cgtttcgttt
     8041 aatgaaaaac aacacgccgg gcaactatac cttcatcctg aaggggacga aggaggtgcc
     8101 acgccgtctg ttgcaggaaa aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat
     8161 tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc taatgctgcc
     8221 aggcagcgaa tttaccgaat ccgatcccga agaaatcaaa gaccgtctgg aaaaacaggt
     8281 cgatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta ttgatcttac
     8341 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataaca
     8401 aattgcgttc cacggatgga agactatggc agggaagttt cgctgcattt tgctgttgat
     8461 agttgggctt tttttctctt cgttaagtta tgcgaaaaac acggagatgc cttcttatga
     8521 agaagggatc tcgctctttg atgttgaagc cactctgcaa ccggatgggg tgctcgacat
     8581 caaagaaaat attcattttc aggcgcgaaa tcagcagatt aagcacggat tttatcgtga
     8641 tttaccacga ctatggatgc agcctgatgg ggacgctgca ctgctgaact atcatattgt
     8701 tggcgtcacc cgtgatggta ttcctgaacc ctggcatctt gactggcata tcgggttaat
     8761 gagtattgtc gtgggcgata aacaacgttt cttgcctcaa ggcgactatc attatcaaat
     8821 tcattatcag gttaaaaatg ctttcctgcg tgagggagat tcagatctgt taatctggaa
     8881 cgtgactggt aaccactggc cgtttgaaat ctataagacc cgattttcac tcaagttccc
     8941 tgatatcgcg ggtaatccat ttagcgaaat cgatctcttt actggagaag agggcgacac
     9001 atatcgaaat ggccgcatcc ttgaggacgg aagaattgaa tccagcgatc cgttttatcg
     9061 tgaagatttc acggtactct accgttggcc tcactcgtta cttagcaatg ccccggctcc
     9121 acaaacgacg aatattttca gccatcttct tttaccctcc acgtcatcgt tgttaatttg
     9181 gtttccgtgt ctattcctgg tttgtggatg gttatatctc tggaagcgca ggccgcaatt
     9241 tacgtcggta gatgttttac ggatgcagaa attttatctg ccgcgtaaaa agtcttcgtt
     9301 ttaccggcct gatacttttt tgcaatgggg tggactggca atattggcgg tcattcttta
     9361 cggtaacctg agtcctgtag gctgggcagg aatgagtctg gtgggcgata tgtttattat
     9421 gatctgctgg cttcttcctt ttttattttg ttcccttgag cttttgtttg cccgcgatga
     9481 tgacaagcct tgcgttaatc gtgtaatcat cactttgttt ttaccgctga tttgttcagg
     9541 cgtggccttt tattctctct atatcaatgt cggagatgta ttcttttact ggtatatgcc
     9601 agcgggttat tttagcgctg ttttcctgac cggttatctc actggcatgg ggtatatttt
     9661 tctgccaaag tttacccaaa ctgggcagca acgttatgcc cacggtgaag ctatcgttaa
     9721 ctatctcgcg cgtaaagagg cagcaacaca cagtgggcgt cggcggaaag gggaaacacg
     9781 gaaactggat tacgcgttgc taggttgggc tgtctcagca aaccttggaa gagaatgggt
     9841 agcacgtatc accccatcac tcacagcggc tgttcgcgcc ccggaaattg cccgtagtgg
     9901 cgttttgttc tcattacaga tgcacctgag tctgggggcc aataccagtt tattggggcg
     9961 aagttattcc ggtggtgctg cgggtggcgg aggcggtggt ggctggtaat taagctgatg
    10021 ttaatcgaaa caagctttaa ccataaagat agtgtagtat gttgcgcctc aaagccaggc
    10081 cagttaaaac gtcgagtcgt ttacttaagg cctgaagagt tcaaacagac tttactatca
    10141 gtgagtcaga tgtaagttct gactcactgt tgcacaaaag catatcagac gcctgggaag
    10201 gcgacaccaa aaggaagctc tatgagcgaa aagctacaga aagtgctggc gcgtgccggc
    10261 cacggttctc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag tgttgatggc
    10321 aaaattgcta aactcggcga tcgcgttgaa gttacccctg gattgaaaat ccgtatcgat
    10381 ggtcacctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct ggcctattat
    10441 aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccaac ggtgtttgac
    10501 cgtctgccaa agctgcgcgg tgcacgctgg attgccgtag gtcgtctgga cgttaacacc
    10561 tgtggtctgt tgctgttcac caccgacggt gaactggcta accgtttaat gcatccaagc
    10621 cgtgaagttg aacgtgaata tgccgtgcgt gtatttggtc aggttgacga cgcgaaactg
    10681 cgtgatttaa gccgtggtgt gcagctggaa gatggtccgg cagcctttaa aaccatcaag
    10741 ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga aggccgtaac
    10801 cgtgaagttc gtcgtctgtg ggaagcggtt ggcgtgcagg taagccgcct gatccgtgtt
    10861 cgttacggtg atatcccact gccgaaaggt ctgccacgcg gcggctggac ggagctggat
    10921 cttgcccaga ctaactatct gcgcgaactg gtggagttgc cgccagaaac cagctctaaa
    10981 gtcgctgtag aaaaagaccg tcgtcgcatg aaggcgaatc agattcgtcg tgcggtgaaa
    11041 cgtcacagtc aggtgagcgg cggacgtcgt tctggtgggc gtaataacgg ttaatcaacg
    11101 tatgccggac gtcatatccg gcatttttac agattagtaa tcaatcccta tctgcgcttt
    11161 tacgccagca tcaaacgcat gtttcacggg gcgtaactca cttaccgtat ccgccagttc
    11221 gagtatatcc cgatgacagc cccgacccgt gataatcacc gtctgctgat gtggacgttc
    11281 atttaacgcc tgcaacactt cttccagtgg caaatagtca tacgccacca tataagtcag
    11341 ctcatcaagc aaaaccaaat ccagtgagga atcagcaagc atccgctttg catgttgcca
    11401 gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg taaagcccgt
    11461 tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca
    11521 ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg cgcgtgttgc
    11581 cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga tgataatacc
    11641 gcgttcatcc tgggcctggg ccatacgagc atctactttt tctttcactc gctgctgacg
    11701 ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg ttgggcgtca
    11761 aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta gagcggcatg
    11821 atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct ggcacgcatt
    11881 gcggtgcgcg taccgcctgg gttaatgcag ttgacacgca ggcgctgctg atattcatcg
    11941 gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc accccagttg
    12001 gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg
    12061 agtaataaag gaagtagtgc ctgggtgagc ataaaggtgg cattaacgtt gacctgtatg
    12121 acgtcctgcc agacctgtgg attttgttcg ctcattggac aaacatcgcc gagtaatccg
    12181 gcattatgca aaacaccatc cagacgcgga taattaacgg caatgcgctg tgccagttgt
    12241 tggcaatctt cggacctgca ggtcagcaaa tcgagaataa accactgtgg ctgacgccca
    12301 gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg gcccaacaga
    12361 atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat accatcactg
    12421 gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg gtaatgcatg
    12481 gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact ttggggcttt
    12541 atgcctgaaa cagaagggca tttcaatcag cctcgggcga taacaccgca aaatggatac
    12601 tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa
    12661 ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg tctgaatatg
    12721 gtttgttttt ggcgaaaatc gttaccgttg tgctagcgat tgcggcgatt gccgccatta
    12781 ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc aacaatctca
    12841 gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac tcacatcagc
    12901 aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag
    12961 cgaaagccaa actgggcgag gtgtcaactg acagtaaacc ccgcgtctgg gtgctggact
    13021 ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata acggcggtac
    13081 ttgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct ggtggcatgg
    13141 tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa aacattccat
    13201 taactgttac ggtagacaaa gttgctgcca gcggtggtta catgatggcc tgtgtggcgg
    13261 acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg gcgcaaatgc
    13321 ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac accgccgggc
    13381 agtataagcg tacgctgacc ttgctgggtg aaaataccga agaagggcgg gagaaattcc
    13441 gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt atgcgtccgt
    13501 ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag gcggtagaga
    13561 agggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg atggaaggcc
    13621 gagaagtggt caatgtacgc tatatgcagc gtaaacgact cattgaccga ttcaccggca
    13681 gcgcggcaga gagcgccgat cgattattgc tacgctggtg gcagcgtggg caaaagccat
    13741 tgatgtaaaa gacaaacgcg agggtaagac ctcgcgtttt gctttaatca accagatgat
    13801 atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg
    13861 ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc acgccattac
    13921 gctgtgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca cgaatgattt
    13981 tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg ttcattattt
    14041 actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa tataccacgc
    14101 tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa gttgcgtatc
    14161 ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt gcttgacatt
    14221 cgaccaaaat ttcgtcgtgc tatagcgcct gtaggccaag acctgttaac tcagtcacct
    14281 gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagagcg agtccatatc
    14341 agtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga agaatcaaat
    14401 taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca aaagccaaaa
    14461 cgatcaacaa gtatctgggt agtgactacg tggtgaaatc cagcgtcggt cacatccgcg
    14521 atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc accaagacgg
    14581 ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt
    14641 ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac
    14701 tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt gaccgcgaag
    14761 gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc
    14821 gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac aaaccgggtg
    14881 agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg
    14941 ggtatatggt ttcgccgctg ctatggaaaa agatcgctcg tggtctgtct gccggtcgtg
    15001 tgcagtcggt ggcagtccgc ctggtggtcg agcgtgagcg tgaaattaaa gcgttcgtgc
    15061 cggaagagtt ctgggaagtc gatgccagca cgaccacgcc atctggtgaa gcgttggcgt
    15121 tgcaggtgac tcatcagaac gacaaaccgt tccgtccggt caacaaagaa caaactcagg
    15181 ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga
    15241 caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct gccagcaccc
    15301 gtcttggatt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat gaagcaggct
    15361 atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta aatatggttc
    15421 gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg aatcagtacg
    15481 ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac gtcaatgtga
    15541 tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag ttaatctggc
    15601 gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg ctgaccgttg
    15661 gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgctttgat ggctggacga
    15721 aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttacctgca gtcgataaag
    15781 gcgatgctct gacgctcgtt gaactgacac cagcccagca ctttaccaag ccgccagccc
    15841 gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta
    15901 cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta gaaaatcgtc
    15961 gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg cctggaagag aatttccgcg
    16021 agttaatgaa ctacgacttc accgcgcaga tggaaaacag ccttgaccag gtggcaaatc
    16081 acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcact cagcagttag
    16141 ataaagctga aaaagatccg gaagaggggg gtatgcgtcc gaaccagatg gttctgacca
    16201 gcatcgactg cccgacctgt ggtcgcaaaa tggggattcg cacagcgagc accggggtat
    16261 tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaaca accattaacc
    16321 tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agacgctgaa accaacgcgc
    16381 tgcgcgcaaa acgtcgttgc cccaaatgcg gcacggcgat ggacagctat ctcatcgatc
    16441 cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac gagatcgaag
    16501 agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggtt
    16561 ctgaaatgca cctgaaaatg gggcgtttcg gtaaatatat ggcctgcacc aacgaagagt
    16621 gtaaaaacac gcgtaagatt ttacgtaacg gcgaagtggc tccaccgaaa gaagatccgg
    16681 taccattacc ggagctgccg tgcgaaaaat cagatgccta tttcgtgctg cgtgacggtg
    16741 ctgccggtgt gttcctggcg gccaatacct tcccgaaatc gcgtgaaacg cgtgcgccgc
    16801 tggtggaaga gctgtatcgc ttccgcgatc gtctgccgga aaaactgcgt tatctggccg
    16861 atgcgccgca gcaggatccg gaaggtaata agactatggt tcgctttagc cgtaaaacca
    16921 aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca gcattttatg
    16981 ttgatggcaa atgggttgaa gggaaaaaat aacctttaat tctgtcaggt ttttataaac
    17041 aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac acctatacac
    17101 taaggctata aatgatatag tggttatagt tagcaccttt tttattatta aatcgtatta
    17161 gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa
    17221 catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc tgaatgtctc
    17281 atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag tcagaatgct
    17341 ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga cgcaggtaac
    17401 gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag ttgatgccat
    17461 aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata tcgccaccac
    17521 gcatacccag gcacgctacg cgttaccaaa cgtcatcaaa ggctttattg agcgttatcc
    17581 ccgcgtttct ttgcatatgc accagggttc gccgacacaa attgctgatg ccgtctctaa
    17641 aggtaatgca gatttcgcta tcgccacaga agcgctgcat ctgtatgaag atttagtgat
    17701 gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc cgctggcagg
    17761 caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat ataccttcgg
    17821 ctttactgga cgttcagaac tggatactgc ctttaatcgc gcagggttaa cgccgcgtat
    17881 cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag ggctgggggt
    17941 aggggtcatt gccagcatgg cggtggatcc ggtcgccgat ccggaccttg tgcgtgttga
    18001 tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta gtactttttt
    18061 gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt
    18121 tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta aagatataaa
    18181 actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg
    18241 gaatatattc tc
//
LOCUS       DAZFKY010000092        18229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFKY010000092 DAZFKY010000000
VERSION     DAZFKY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(27..785)
                     /gene="aroD"
                     /locus_tag="ACHKCL_002771"
     CDS             complement(27..785)
                     /gene="aroD"
                     /locus_tag="ACHKCL_002771"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5763711.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(816..1682)
                     /gene="ydiB"
                     /locus_tag="ACHKCL_002772"
     CDS             complement(816..1682)
                     /gene="ydiB"
                     /locus_tag="ACHKCL_002772"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5763712.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1694..2959)
                     /locus_tag="ACHKCL_002773"
     CDS             complement(1694..2959)
                     /locus_tag="ACHKCL_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763713.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPKNDVRFGERKFSTRLNTIKHRG"
     gene            complement(3186..4400)
                     /locus_tag="ACHKCL_002774"
     CDS             complement(3186..4400)
                     /locus_tag="ACHKCL_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763714.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4500..4856)
                     /locus_tag="ACHKCL_002775"
     CDS             complement(4500..4856)
                     /locus_tag="ACHKCL_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5763715.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4992..5099)
                     /gene="rprA"
                     /locus_tag="ACHKCL_002776"
     ncRNA           complement(4992..5099)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKCL_002776"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5285..6397)
                     /gene="ydiK"
                     /locus_tag="ACHKCL_002777"
     CDS             complement(5285..6397)
                     /gene="ydiK"
                     /locus_tag="ACHKCL_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5763716.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            6786..9842
                     /locus_tag="ACHKCL_002778"
     CDS             6786..9842
                     /locus_tag="ACHKCL_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5763717.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9839..10249
                     /gene="menI"
                     /locus_tag="ACHKCL_002779"
     CDS             9839..10249
                     /gene="menI"
                     /locus_tag="ACHKCL_002779"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5763718.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            10349..10537
                     /locus_tag="ACHKCL_002780"
     CDS             10349..10537
                     /locus_tag="ACHKCL_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5763719.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11086..11454
                     /gene="sufA"
                     /locus_tag="ACHKCL_002781"
     CDS             11086..11454
                     /gene="sufA"
                     /locus_tag="ACHKCL_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5763720.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11463..12950
                     /gene="sufB"
                     /locus_tag="ACHKCL_002782"
     CDS             11463..12950
                     /gene="sufB"
                     /locus_tag="ACHKCL_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5763721.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12960..13706
                     /gene="sufC"
                     /locus_tag="ACHKCL_002783"
     CDS             12960..13706
                     /gene="sufC"
                     /locus_tag="ACHKCL_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5763722.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13681..14952
                     /gene="sufD"
                     /locus_tag="ACHKCL_002784"
     CDS             13681..14952
                     /gene="sufD"
                     /locus_tag="ACHKCL_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5763723.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            14949..16169
                     /gene="sufS"
                     /locus_tag="ACHKCL_002785"
     CDS             14949..16169
                     /gene="sufS"
                     /locus_tag="ACHKCL_002785"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5763724.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16182..16598
                     /gene="sufE"
                     /locus_tag="ACHKCL_002786"
     CDS             16182..16598
                     /gene="sufE"
                     /locus_tag="ACHKCL_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5763725.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16747..17751
                     /gene="ldtE"
                     /locus_tag="ACHKCL_002787"
     CDS             16747..17751
                     /gene="ldtE"
                     /locus_tag="ACHKCL_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5763726.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17814..18050)
                     /gene="lpp"
                     /locus_tag="ACHKCL_002788"
     CDS             complement(17814..18050)
                     /gene="lpp"
                     /locus_tag="ACHKCL_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5763727.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4784 a         4419 c         4678 g         4348 t
ORIGIN      
        1 ggatattccc gccgaaatat tattgcttat gcctgatgta aaatagttaa tactgtgcgc
       61 aaatcattta ccgagatttg ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc
      121 gccgagccaa atacttcacc agccagacga gaaattacgc cagtttttgc cattgacatc
      181 gtgatgattg gacgatcggc atactgctcc tgcatctcca gggtcgcggc aagcaacgcc
      241 agcacatcgc tggtactttg cggcatcagc gcaatcttag gaatatcggc gtcgaaggac
      301 tgcattttgc gcagacgggc aatgatttct tcggcttccg gcgttttatg gaagtcatgg
      361 ttggacatga caactttcac atcatgcgcg tgggcgtagg cgacggtttc tttaacctga
      421 tcatcgccgg taaataactc cagatcgatc atatcaacca ggccgctgtc gatggctgca
      481 cgattgagag caatataagc ctcggtggaa atcgcctgct cgccgccttc tttggcactg
      541 cggaaggtaa acagcagcgg tttttctggc atggtttcac ggagaatttt tgccgccgcc
      601 atgacagact ccacattgga gaggtcggca aagtggtcca cacgccattc cagaatatca
      661 aagtccgctt cacgataggc gagagcttcg gatttcactc gggcgatatc tttcgccatc
      721 agcgagacga tgattttagg tgcgcccgta ccaatgacga gatcttttac agttacggtt
      781 ttcatttttt accctttctg cacgcggtca gcctgtcagg caccgaaccc catgacctgt
      841 ttaacatatt ccagagggaa atctttgcca gtccataacg tgaactgttc agccccttgc
      901 cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag cttgttgcgc ctgctgcaat
      961 aacttcgtca tatgcgggtt atacacgcat tcagtgacca gcagtcccgg atgtaacaga
     1021 ctgatatcat taaccaatga tttattctca aggggtttca tacccacttt tgtgccattg
     1081 gttaaaatgt cggcggaagc cagggcttca gcaaaggctt gctgatcggc gagatcggta
     1141 accgtgacga cacaatcggt gttttcgtta acccgctgcg cgaaggcgag ggctttatcg
     1201 aagaacttat cccgacggtt aaagagttta atttctttta aaccttcaat tgccccctgc
     1261 gcgccaatcg ccgttgaggc acctccggcc cctaacagca ccattgtttt gcctttgata
     1321 tcaaaaccgc tctctttaat ggcgcgaata tggcccgtgc cgtcggtgtt atagccgcgc
     1381 aggtagccat catcattaac gatggtgttg atggcaccca ccagtttggc tgctggcgtt
     1441 aattcatcaa catattcaca cgccagttgt ttgttcggca ttgatacacc agttccgcgc
     1501 atttttagag cttttaatcc ttcaattgct ccgggaaagc tatcgttatc cacttcgaag
     1561 gccatatagg taaatggcaa tcccgctttt tctaaggctt tattctgcat ttcaggcgat
     1621 aaactgtggc ggataggata agccatcaac ccaatcaatt cgtattttgc ggtaacatcc
     1681 ataattaact cctttaacct ctatgcttaa ttgtgtttaa ccgggtgcta aatttgcgct
     1741 cgccaaaccg cacatcattc ttaggaataa taaaaacgcg gtaatagcgg ataaaaacaa
     1801 taattgcggt aatcagggcc agcagcgcga aagtaaaatc gagaacaatg atatattgca
     1861 ggccgatgtt cgacagataa ccggtaatca gcggaataac aaagttagcc agtccaccca
     1921 tcatcatata aatactggtg actttggctt tgctttttgg gaaaaactct gacatgaccg
     1981 aaacaccgag ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag gcgccggcat
     2041 tacacaccag tggagaaggg tacaggtaga taatggctgc tgttattgtt gccagtgcag
     2101 agttaaatac attagcccag atgggccgga ccattttttt cagtagtgcg gcaaaaataa
     2161 agacacagac cagcgagccc atactgtaat aagagatggt ttttaatgcc tcagcttctg
     2221 acataccagc aaaagccatc gcatatttgg gcatccacac cacaatcaca taaaaggtcg
     2281 agaatgcggc tacaccgaac agtaccgatg aaacaccttc cagccagact aacggtttgc
     2341 tgttcatttg cggtaattca ttagctacgc tggcgtccac caactggctg gggaatttgc
     2401 ttttcaacag catcagcgtg atcagtacaa acagaatacc cggaataatc agcccatagc
     2461 cgtaccagat attattgagc aacatatagc tcaccagcat tgggtagaac atttgcccaa
     2521 atgacaccat cgctttaacc agtatgaccg ccgaaccaga ggctttcgga aagcattcca
     2581 tgagcgcggg gtagccaccc gtatccagcg ctgagttagc gatacctacg cacactgcca
     2641 gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat agcagataca
     2701 ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc acaccgaaga
     2761 ataaaatact gatcaatcgc cccaaaccga taccggaaat taagtaggca atgcccgcgt
     2821 tgtcagtgga aaacttttcc gccagagatg acatattttg ggcaagcgta ataacactaa
     2881 tgccgtgcag gaagtagctg aagtaaatac aaagaacagc caggataaat ggcgtgctga
     2941 aagccttatt ttgagacatt ttcacagctc cttgctgaaa gataatttcg ttaccgtacg
     3001 tattgttctc cgacggttta cataattact atactgaaga tgattttccc tgggtacatg
     3061 gcataaagtt tatgtttcca ggaatattgc atgtcatagt ttgtttttga tcgcgatttt
     3121 ccttagttag cgcagaaaga gttatttatc cgtaagatct tgcatgacct gaaaagaata
     3181 ttgcattacc cacccggagc gacattttcc tttagcgagt gatgccgcgt ttttttgcgg
     3241 ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagcgatggc ggtatcgaac
     3301 cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg aatagtaaag
     3361 gtcgcaatac tgcccgcact gtaatagatc cctgtagctt tacctttagc gtaagggaaa
     3421 cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaagt aaaaccaatg
     3481 acaaaagaaa atattatcac cacataaaat gtgggatgca ggcagacggt aaacagagca
     3541 ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt attacgaatg
     3601 agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact gagtagtttg
     3661 atcgacatag tgtatgacat gcctgcaaca aattgtccgt actgtgccag ccactggcta
     3721 accagataaa acgttgccat tgagatatag ccatataagg tataactggc taaatcgata
     3781 attgaacagc gatgttccgt agagctggtg gttttcttta tgacaggtaa gcgacgaccc
     3841 ggatggggtg ggaacgtaca acggtataaa aacagagcgt taataaacat aatgcctgca
     3901 gcaatcataa aggaccaacc gaaccacagt tcagcccaca ctaacaggct aatgattagc
     3961 ggtaataaaa attgtccgct ggaaacaaat gctttaatta aaatattggc tgtcccaggt
     4021 gagcgtggaa aagcttccat caaactggga taagtgcctg catcgagaaa actgtttgcc
     4081 attcccgcca gaaagccaaa aacataagcg ataatgatgt tattggtctg caggatgcca
     4141 aaaaagaagg ccatatagca gcacatcccg agcatgataa aagggcggcg accaaagcga
     4201 tcggataata atcctgcaaa aagcaagaca cttaatcgac caatgcccag cgatgagata
     4261 actatcgaga cacccgcggc attagactgc caaagtgtct ccagcgaggc catattcagg
     4321 ctcatcaaaa tgacgcccat accatgcacc aggtaattaa aatacaaccc aagtgcggta
     4381 gggtaatagg gatttttcat aggttctact tagcaaaggt gctttaaaaa gtgtagcgat
     4441 gttgtgtcag tatccgttca ttattgttgt aacaacgaca attccttttt gtggagaaat
     4501 taacaaagcc gctccagatc gtgtgcatat aattcagcgg ctaccgattg atagatcttc
     4561 cagtcaataa aattgccatc agggtagact tgttgaaacg cggttaagtc ggggaaaaaa
     4621 cgcatggtgt tgttgccgat gcggttattt attttctcta tgatcgcatg aagatgtttt
     4681 tttctttgct gacgcatagc caacagttgc tcgactacac aatcaggaat ggcacacctg
     4741 ccattttccc attgacgcca gctttcactg tcaccggtct gggcaatcca ggtcgcgcat
     4801 tcatcaatag tcatggcaaa aatatggcgg agtgcctgga gttcataagc gttcatcgtg
     4861 taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg ctgacggctt
     4921 gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa
     4981 gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg caaagactac acacagcaat
     5041 tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt tataaccgtg
     5101 agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt agtttaaaaa
     5161 ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga aaatatgggg
     5221 aagagcaagc aaatatcaat tagcggtggt taacaatcag gcggcagagc cgcctgatga
     5281 gaaattactt attcggtttc tggatttcgc caagttcttc gagaatttct tccggttgat
     5341 ccgtcggcgg tgggacctct tccacccacg cggcaaacag acgccaggaa acggctaaca
     5401 gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca ataacgccag
     5461 agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg cggatgacgt
     5521 tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag gtggtatcgc
     5581 cagtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca agctggacaa
     5641 ggcaggaaag gatcattaac accgttagca aagttgcata aggtacgccg gatacggcga
     5701 ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc agcgccaccg
     5761 cgcggatagc ctgtgccgcc agcagtacgg cggcatctcc gcgaacgcct gccagacggg
     5821 tcgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc agggcactga
     5881 agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgct tgcccaacga
     5941 accaggtagt ggtggtgcca atataagggc ggactttcgc catgatcgcc gtccccccca
     6001 tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga atggtattaa
     6061 gccacgccag atcgggtaac gtcatgtcac cgctggaaat ggctttaatt agcgggccgc
     6121 tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc atcactaata
     6181 acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt tgcaaacgta
     6241 acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg agaataaagg
     6301 gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc gaaagcagaa
     6361 tttgtgcgac atccctgggc tgacgaacat ttaccataac tacttttcac cttttttgtg
     6421 cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga tgagcaattt
     6481 cagcggtttt taacaggccg attctgccct taattctgat gggcgtgtta gaaaaaaatg
     6541 tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa gttgcatatg
     6601 ctgcggctac gttcgttcac cccagttact tactgttgta agctcctgga gttcactcgc
     6661 ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt tctgtgcgct
     6721 attgcagccg cctgaaagaa cagtgtatcg acctacatca cagaccgcag gaaagggtca
     6781 atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg cttaattttt
     6841 tgcaagagct ggagcaacaa ggttttaccg gcgatacggc gacaagttat gctgatcgtc
     6901 tgacaatgtc gaccgacaac agtatttacc aacttctccc cgatgcagtg gtatttccgc
     6961 gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc
     7021 tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc aaccagggta
     7081 ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct gaagagggct
     7141 gggtgcgcgt tgaggccggg gtgataaaag accaactcaa tcagtacctg aaaccgttcg
     7201 gctacttttt tgcaccagaa ctttcgacca gcaaccgggc aacgctcggc gggatgatca
     7261 ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat cacgtacttg
     7321 gcctacgtgc ggtattgttg gggggcgata ttctcgatac gcaaccttta cccgtcgagc
     7381 tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac acggtttatc
     7441 aacgttgccg tcagcaacgc cagttaatca tcgacaactt ccccaaactt aaccgctttc
     7501 ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc gatctgacgc
     7561 gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg ctggatatta
     7621 cgcgcttgcc taaagtgcgc cgtctggtga atgtcaaata tgactctttt gactctgcgc
     7681 tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg gtggactcaa
     7741 aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag ttgattaccg
     7801 atgtgcctga caaagagatg ctcgggctga acattgtgga atttgctggt gatgatgagg
     7861 cgctgattga cgagcgggta aatgcactct gtgcgcggtt ggatgagctg atcgccagtc
     7921 atcaagcagg tgtcatcggc tggcagatgt gccgtgagct ggcgggggtt gaacgtatct
     7981 atgcaatgcg caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa
     8041 ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct ggccgattat attgctgaat
     8101 ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac gtcgacgcag
     8161 gtgtcttgca tgtccgtccg gcactggata tgtgcgatcc gcaacaagag attttgatga
     8221 agcaaatctc tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg
     8281 agcacggcaa aggttttcgc gctgaataca gcccggcgtt tttcggtgag gaactttttg
     8341 cagaactgcg caaagtgaaa gcggcatttg acccgcataa ccgactcaac ccagggaaga
     8401 tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta
     8461 cattcgatcg gcagatcccc attgcggtac gccagcagtg gcgcggtgcg atggagtgta
     8521 acggcaacgg tttatgcttc aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga
     8581 tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc
     8641 gtttgctggc ggatcgcggc gttgatccac tcaaactgga acaagaactg cctgaatccg
     8701 gcgtcagctt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg aataaaggcg
     8761 aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagctt
     8821 gttcgaccca gtgccccatc aaaattgatg tgccggagtt tcgctctcgt tttctgcagc
     8881 tctatcacac ccgttattta cgcccgctgc gcgaccacct cgtcgctacg gtcgagagct
     8941 acgcgccgct gatggcacgc gcgccgaaaa cctttaactt cttcattaac cagccgctgg
     9001 tgcgcaaact ctcggaaaaa catatcggca tggttgattt gccgctgctg tcggtcccct
     9061 cgctacaaca acaaatggtg gggcatcgct cggcaaacat gacgctggaa cagcttgaat
     9121 ctctcaatgc agagcagaaa gcgcgcacag tgttggtggt gcaggacccc tttaccagct
     9181 attacgatgc gcaagtggtg gcggattttg tccgtctggt cgaaaaatta ggtttccagc
     9241 ctgtgttact gccattttcg ccaaatggca aagcccagca tattaaaggt tttcttaatc
     9301 gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa ccgtatggcg aagctgggta
     9361 tgccaatggt gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa tataaactgg
     9421 cgctgggcga ggagcgtggc gagtttaacg tcttactggc gaatgaatgg ctggcaagcg
     9481 cacttgagtc acagccagtg gctacagtca gcggtgaatc atggtatttc tttggtcact
     9541 gtaccgaagt taccgccttg ccgggtgcgc cagcacaatg ggccgcgata tttgcccgtt
     9601 ttggcgcgaa actggaaaat gtcagcgtgg gttgctgcgg catggcaggg acttacggac
     9661 atgaagcgaa aaaccataaa aattcgctcg ggatctatga gttatcctgg catcaggcta
     9721 tgcagcgact gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt agtcaggtca
     9781 aacgggttga aggcacgggg gtacgccatc ctgtgcaggc tttactggag attattaaat
     9841 gatatggaaa cgaaaaatcc ccctggaagc actgaatgcc atgggtgaag gaaacatggt
     9901 ggggttactg gatattcgct ttgaacatat tggtgatgac acccttgaag cgacaatgcc
     9961 agtagactcg cggacaaagc agcctttcgg gttgctacat ggaggtgcat ccgtggtact
    10021 ggccgaaagt atcggttccg ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt
    10081 tggtctggaa atcaatgcta accacgtccg ctcggcacga gaagggcggg tgcgcggcgt
    10141 atgcaaaccg ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa tctccgatga
    10201 gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac gttgtcacca
    10261 gaaaagtgtg acgcatattg cttttggtca ttatctgcac agtaaacggt ggtatactgt
    10321 gcaggtcttg tctaaaagcg aggatgctat gtctactcaa cttgatccca cccagctggc
    10381 cattgaattt ttacgccgtg accagagcaa tctttcacct gcccaatatc ttaaacgtct
    10441 gaaacaacta gaacttgaat ttgctgacct gcttactctc tcttctgctg aattaaaaga
    10501 agaaatctac tttgcctggc gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc
    10561 gccaaatcga cctggattaa cctccgtcca ggttcgatga ccttcttaat actgcttatc
    10621 tacccgcctg atattgttca aaaataatac taatcgcagt ttgtgttaaa acggcggctt
    10681 agctttatga gattattctt atcgcccctt caagagccaa gccactgaga gtgccggaga
    10741 taagcgccgg atggggtaga aacccttaag cctgtgtcgc acagacttaa gggttttctt
    10801 atttctgaac gctgcacttt tttacctatc tcttaaatag tcattttcaa tacaactttt
    10861 tctttttcat cacagcctta atgctcctta gataataatc attatctaac aatgagatac
    10921 ctaattctta gcgtgcctgt taacccacac atcagggtct atgcttatta aaagcaggca
    10981 aaaagcatgt tttacggtaa agcccctgcc tttgctgggt tgaactgata atcattatca
    11041 ctaacatgct gttatacgct gaaagcgatg aagtgaggta aatcgatgga catgcattca
    11101 ggaaccttta atccacagga tttcgcctgg caaggcttaa cgctgacacc cgcagccgct
    11161 gtccacatcc gcgagctggt ggcaaagcag ccgggtatgg tcggcgtgcg cttaggtgtg
    11221 aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca gtgttagcga gccggacaaa
    11281 gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca agcgatgccg
    11341 tttattgatg gcacggaagt cgatttcgtt cgtgaaggac ttaatcagat attcaaattt
    11401 cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa gctttggggt ataggcggta
    11461 ctatgtctcg taatactgaa gcaactgacg atgtcaaaac ctggaccggc ggcccgctga
    11521 attataaaga aggattcttc acccagttag ccaccgatga gctggcaaag gggataaacg
    11581 aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc
    11641 gtctaaacgc ctatcgcgca tggctggaga tggaagaacc gcactggttg aaagcgcact
    11701 acgacaagct gaattatcag gattacagct actactcggc accatcgtgc ggtaattgtg
    11761 acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa
    11821 gtaaagaggt ggaggcggcg tttgagcagt tgggtgttcc cgtgcgggaa ggcaaagagg
    11881 tggcggtgga tgccattttc gactcagttt cggttgccac tacttatcgc gaaaaactgg
    11941 cggagcaggg aattattttc tgttcctttg gtgaggcgat ccacgatcac ccggaactgg
    12001 tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa cttctttgcc gcgcttaatg
    12061 cggcggtagt ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg
    12121 aactttccac ctattttcgc attaacgcgg aaaaaaccgg acagtttgag cgcaccattc
    12181 tggtggccga cgaagacagc tacgtcagct acattgaagg ctgttccgct ccggtgcgtg
    12241 acagctatca gttgcacgcg gcagtggtgg aagtcatcat ccataaaaac gccgaggtga
    12301 aatattccac ggtacaaaac tggttccctg gcgataacaa caccggcggt attctcaact
    12361 tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag
    12421 aaaccgggtc agcgattacg tggaaatatc ccagctgcat tttgcgcggc gataactcca
    12481 ttggtgagtt ttactcagtg gcgctgacca gcggtcatca gcaagcggat accggcacca
    12541 agatgatcca catcggtaaa aacaccaaat cgaccattat ctcgaaaggg atctctgccg
    12601 gacatagcca gaacagttat cgcggcttag tgaaaatcat gccgacggca accaatgcgc
    12661 gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg cataccttcc
    12721 cgtatgtcga atgtcgcaat aacagcgcac aactggagca cgaggcaacg acatcacgta
    12781 ttggtgaaga tcaactgttt tactgcctgc aacgcgggat cagcgaagaa gacgccatct
    12841 cgatgattgt taacggtttc tgcaaagacg tgttctcaga actgccgctg gaatttgccg
    12901 ttgaagcaca aaaactcctc gccatcagtc ttgaacacag cgtcggataa ggaataaaca
    12961 tgttaagtat taaagattta cacgtcagcg tggaagataa agctatcctg cgtggattaa
    13021 gcctcgacgt tcgtcccggc gaagttcacg ccattatggg gccaaacggt tcgggcaaaa
    13081 gtaccttatc ggcaacgctt gccgggcgag aagattatga agtgacgggc ggcacggttg
    13141 agttcaaagg caaagatttg cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct
    13201 ttatggcctt ccagtatccg gtggagattc caggtgtcag taaccagttt ttcctgcaaa
    13261 cggcacttaa tgcggtgcgc agctatcgcg gccaggaaac gctcgaccgc tttgattttc
    13321 aggatttgat ggaagagaaa atcgctctcc tgaagatgcc ggaagattta ttaacccgtt
    13381 cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg
    13441 tgctggaacc ggagttatgc attcttgatg aatcggactc cgggctggat atcgacgcat
    13501 taaaagtggt cgccgatggc gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg
    13561 ttacgcacta ccaacgcatt ctcgactaca tcaagcctga ttacgttcat gtgctatatc
    13621 agggacgaat tgtgaaatcc ggcgatttca cgttggtcaa acaactggag gagcagggtt
    13681 atggctggct taccgaacag cagtaacgcg ctgcaacagt ggcatcactt gtttgaagct
    13741 gaagggacaa aacgctcccc gcaagcacag cagcatttac aacaattgct gcgtaccgga
    13801 ctgccgacac gtaaacatga aaactggaaa tatacgccgc tggaagggct gaccaatagt
    13861 cagtttgtca gcattgcggg agagatatcc ccacagcagc gagatgcctt agcgttaacg
    13921 ttagacgctg tgcggctggt atttgtcgat ggacgttacg tgcctgcgct gagcgatgcg
    13981 actgaaggca gcgggtatga agtgagcatt aacgacgacc gtcaggggtt acccgatgct
    14041 attcaggcgg aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc
    14101 gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt taatgcatat cacccagggc
    14161 gtggcaggtg aagaggtgaa cactgcccat taccgacatc atctggagct ggcggaaggt
    14221 gccgaagcaa cggtgatcga acattttgtc agccttaatg atgctcgtca ttttaccggg
    14281 gcacggttct ctatcaacgt cgcagcgaat gcccacttgc agcatatcaa gctggcgttt
    14341 gaaaacccgc tcagtcacca ctttgcccat aacgatttgt tgctggctga cgatgccacc
    14401 gcatttagcc acagtttcct gctgggtggc gcagtgttac gacacaacac cagtacgcaa
    14461 ctcaatggcg aaaactgcac gctgcggatc aatagcctgg cgatgccggt gaaaaacgag
    14521 gtgtgtgata cccgtacctg gctggaacac aataaaggtt tttgtaacag ccgacagttg
    14581 cacaaaacta tcgtcagcga caaaggccgc gcggtattta acggtttgat caacgtcgcg
    14641 cagcacgcca tcaaaacgga tggtcagatg accaacaata atctgctgat gggcaaactg
    14701 gcggaagtgg atacgaaacc gcagctggaa atctatgcag atgatgtgaa atgcagccac
    14761 ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct atctgcgctc gcgcgggatc
    14821 aatcagcagg atgcccagca gatgatcatt tacgcctttg ctgccgaact gacggaagca
    14881 ctgcgtgatg aggggcttaa acagcaggtg ctgacccgaa tcggtcaacg gctgccagga
    14941 ggtgcaagat gactttttcc gtcgacaaag tgcgggccga ctttccggtg ctttcgcgtg
    15001 aggtaaacgg tttgccgctg gcttatctcg acagcgccgc cagtgcgcag aaaccgagcc
    15061 aggtgattga cgccgaggcc gagttttatc gtcatggcta cgcggcggtg catcgcggta
    15121 ttcatacctt aagcgcccag gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc
    15181 tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg acggaaggga
    15241 tcaatctggt cgccaatagc tggggcaaca gcaacgtgcg ggcgggcgat aacatcatca
    15301 taagtcagat ggagcaccac gctaacattg ttccctggca gatgctttgc gcacgcgttg
    15361 gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac gttgcaactg gagacgctgc
    15421 ctacgctgtt tgatgagaaa actcgcctgc tggcaattac tcatgtctcc aacgtgcttg
    15481 gcacagaaaa tccactggcg gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc
    15541 tggtggatgg cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg ctggattgcg
    15601 atttttacgt gttttccggg cataaactgt atggccccac cgggattggc attctttatg
    15661 tcaaagaagc cttgctacag gagatgccgc cgtgggaagg gggcggttct atgatcgcca
    15721 ccgtcagcct gagtgaaggc actacctgga ccaaagcacc gtggcggttt gaagccggta
    15781 cacccaatac cgggggcatc attggtcttg gcgcggcgct ggagtatgtt tcggcgcagg
    15841 ggcttaataa catagccgag tatgaacaga atctgatgca ttacgcgcta tcacagctgg
    15901 aatctgtacc ggatctcact ctctatggcc cacaaaacag gcttggcgtt attgctttta
    15961 atctcggtaa acaccacgcc tatgatgttg gcagttttct cgataattac ggcattgctg
    16021 tgcgtaccgg acatcactgc gctatgccat taatggccta ttataacgtc cctgctatgt
    16081 gtcgggcgtc gctggccatg tataacaccc atgaagaagt ggatcgtctg gtgaccggac
    16141 tgcaacgtat tcaccgtctg ctgggataac agggaggcac tatggcttta ttgccggata
    16201 aagaaaagtt gctgcgtaat tttttacgct gcgccaactg ggaagagaaa tatctctaca
    16261 ttattgagct gggccagcgt ctgccagaat tacgcgacga agacagaagc ccacaaaata
    16321 gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg tcagaatgcg cagggaatta
    16381 ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta
    16441 ttctctacga tcagatgacg ccgcaggatg ttgtcaattt cgatgtgcgt ccgtggtttg
    16501 aaaaaatggc actcacccaa catctcaccc catctcgttc acaaggtctg gaagcgatga
    16561 ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac taaagggaca gctttcatcc
    16621 ggttggctca cattgccgga tggtcaatgt ttacactgac ctgcacagga gtgtttcggt
    16681 ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag tctccggcaa atacaggagg
    16741 tttacaatga aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt tagtgccatc
    16801 caggctgcct gggcggttga ttatccgcta ccaccaaccg gaagccgact ggttgggcaa
    16861 aatcaaacgt atacggtgca ggaaggggat aaaaaccttc aggccatcgc ccgacgtttt
    16921 gatactgcgg caatgttgat ccttgaagcc aataacacta tcgccccggt gccaaaaccc
    16981 ggtacgacga taactattcc ttcgcaactg ttattacctg atgcgccgcg tcaggggatt
    17041 atcgttaacc ttgcagagct gcgcctttat tattatccgc cgggagaaaa tatcgtgcag
    17101 gtttatccga taggtattgg cttgcagggg ctggaaacgc cagtgatgga aacgcgtgtg
    17161 gggcagaaaa tccctaaccc aacctggacg cctacggcag gcattcgtca acgttcgctg
    17221 gagcgtggca ttaaattacc gccagtcgtt cctgccggac caaataaccc gctaggacgt
    17281 tacgcactgc gcctcgcgca tggtaatggc gaatacctca ttcatggcac cagtgcgccg
    17341 gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc ggatattaaa
    17401 gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga accagtgaaa
    17461 tattccgtgg agcctaacgg gatgcgttat gttgaagtac atcgaccact atcggcagaa
    17521 gaacagcaga acgttcagac aatgccatac acactgccag caggctttac gcaatttaaa
    17581 gacaataagg ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat
    17641 ccggttgcgg tgagcagtgg agcaactccc acagccagca atgcgccttc agtagagtca
    17701 gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg cgacgcagta gtggtaaata
    17761 gcagacaaaa aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg
    17821 cggtatttag tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca
    17881 gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc
    17941 agagtctgaa cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc
    18001 agagtagaac ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc
    18061 ctctagattg agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg
    18121 agaatatttt tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa
    18181 cgctgggttt tagataaaaa aattgagaga aaacaggatg cttccatcg
//
LOCUS       DAZFKY010000093        18194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFKY010000093 DAZFKY010000000
VERSION     DAZFKY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2244
                     /locus_tag="ACHKCL_002789"
     CDS             <1..2244
                     /locus_tag="ACHKCL_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO5763728.1"
                     /translation="LFFMTGNACATEWISSEDLITSDFHLMTADERNVVKAATDDSME
                     AAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYLSGNGHLIVLGNV
                     IVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFEVIKAE
                     FYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADNIVQGL
                     PLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVMLQLLQ
                     HPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADVNKKLMSVPVESV
                     QLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELINNEDPELRE
                     RITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTEDIERIAEQMYLD
                     NKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQHDVPTVWD
                     ELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEEMLNNAID
                     LLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSER
                     GLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLT
                     ESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVKTGATRKIRNEAR
                     HRLEEKQ"
     gene            2241..2690
                     /locus_tag="ACHKCL_002790"
     CDS             2241..2690
                     /locus_tag="ACHKCL_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763729.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(2775..3686)
                     /gene="rdgC"
                     /locus_tag="ACHKCL_002791"
     CDS             complement(2775..3686)
                     /gene="rdgC"
                     /locus_tag="ACHKCL_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5763730.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3811..4719
                     /gene="mak"
                     /locus_tag="ACHKCL_002792"
     CDS             3811..4719
                     /gene="mak"
                     /locus_tag="ACHKCL_002792"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5763731.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4862..6046)
                     /gene="araJ"
                     /locus_tag="ACHKCL_002793"
     CDS             complement(4862..6046)
                     /gene="araJ"
                     /locus_tag="ACHKCL_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5763732.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(6172..9315)
                     /gene="sbcC"
                     /locus_tag="ACHKCL_002794"
     CDS             complement(6172..9315)
                     /gene="sbcC"
                     /locus_tag="ACHKCL_002794"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5763733.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9312..10514)
                     /gene="sbcD"
                     /locus_tag="ACHKCL_002795"
     CDS             complement(9312..10514)
                     /gene="sbcD"
                     /locus_tag="ACHKCL_002795"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5763734.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10704..11393
                     /gene="phoB"
                     /locus_tag="ACHKCL_002796"
     CDS             10704..11393
                     /gene="phoB"
                     /locus_tag="ACHKCL_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5763735.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11451..12746
                     /gene="phoR"
                     /locus_tag="ACHKCL_002797"
     CDS             11451..12746
                     /gene="phoR"
                     /locus_tag="ACHKCL_002797"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5763736.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            13153..14472
                     /gene="brnQ"
                     /locus_tag="ACHKCL_002798"
     CDS             13153..14472
                     /gene="brnQ"
                     /locus_tag="ACHKCL_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5763737.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14548..15921
                     /gene="proY"
                     /locus_tag="ACHKCL_002799"
     CDS             14548..15921
                     /gene="proY"
                     /locus_tag="ACHKCL_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5763738.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16077..17894
                     /gene="malZ"
                     /locus_tag="ACHKCL_002800"
     CDS             16077..17894
                     /gene="malZ"
                     /locus_tag="ACHKCL_002800"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5763739.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            18082..>18194
                     /locus_tag="ACHKCL_002801"
     CDS             18082..>18194
                     /locus_tag="ACHKCL_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763740.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFKKST"
BASE COUNT         4228 a         4480 c         4847 g         4639 t
ORIGIN      
        1 ttatttttta tgactggcaa tgcatgtgcc acagagtgga tcagtagcga ggaccttatc
       61 acctctgact ttcatcttat gacggcagat gagcgcaacg tggttaaagc ggcaaccgac
      121 gatagtatgg aagctgctta tatgctaaaa gataatatcc gctggtatta tcataatggt
      181 gacctatcat tacccgctaa cttcagcaat aaaaataaac tggtggtgaa cggtaattta
      241 acgatttccg gcgattatga tgattatctg tcaggtaatg gtcatctgat tgtattaggt
      301 aatgttattg tcgataactt tattaatcat gatttcgcgt atgtcaaagg acagatgaca
      361 gcgaaaggtt tagtttatgc tgattacaat gatcataact ttgaagtgat gaagggaatt
      421 agtgcgcgag gcattattgt ttccgataaa gcaacgcaat ttgaagtcat taaagctgaa
      481 ttttatatca atgaagatgg aagtggtgaa ggatataact gggatgagaa tattcaaaaa
      541 gcctattcac tggtgactgc cgatctttat gatcacactg aaatcgaaac cgataacatt
      601 tccaatgcgt atcctgacta tgatagcgtc gcagacaaca tcgttcaggg tttaccgctt
      661 tttcgagata aagccgctcc tgaaattaat gagaaattaa aatggattga aacggggaaa
      721 ctcgataatt tcccagcgaa taaaataaag catcaagatc cactggtggc acgttttctg
      781 actcatacag agagtttatc acccgccgtt atgctgcaac tgttacagca ccccgatgac
      841 cagacgcgtg aatcgatggc gcagtcctgg cccgctcaac agatgcattg gcttaccgat
      901 gaattaatta aagatgaggc tgttgccaga gggctggtta aaaacagtaa tatttccgcc
      961 gatgtgaata aaaaattaat gtctgtaccc gtcgagagcg tacagctgga gcaggccaga
     1021 caggataatc tttctccaga tattgttgcg tcgttaagtc atagcccctt tctcagtgta
     1081 cgcaagacat tactcagcca ttacgattac gcctggctgg ttccgactgc agtggccgat
     1141 gagttgataa ataacgaaga cccggagtta cgtgaaagaa ttaccggagc agatttaacc
     1201 gcacaacaag cagtgatgtt gagtaaagat aaatcactga aagttcgtga agcattagcc
     1261 cggacgctta ctgaattaaa aattacccaa ctgtcagcaa ccctgcgtac agaggatatt
     1321 gaacgtatcg cagaacaaat gtatctggat aataaagaaa ataaaaatat cgtaaaggtg
     1381 ttattgatag cgttgccgga gatgcgtcaa ctgtctctgg caaaagaaga tgtgcacaat
     1441 ttgcgcgaag gtgcgcgtta tctgacatcg aaggacgtga ttagttatct attgactcag
     1501 catgatgttc ccaccgtctg ggatgagctg gcacgcgata aattattgcc gctggaatat
     1561 aagaaacagt tatggcaacg cacattaaat ttaatgatga gcaaacggca agaagatcaa
     1621 gagcaagcct acgaagtaca gctggcgctg attgataatg gtgttgttga cgaagagatg
     1681 ttaaataatg ccattgattt actggttgat ttacccgctg aataccgcta tcgaatgcgt
     1741 aaccaattat ttgataataa agaattacct tccggaatta tcaacaaact cgatcaacag
     1801 tatcgcttca actctgactg ggcgctggct gttgtcagta tgaaaaatag cacccgtaga
     1861 caaagtgaaa gaggattaca ccgctggaac catgaggata gcgatatttt tgcggaactg
     1921 gcgacgataa aagataaatc agatgatgaa tggtggcgtg cattgctgca aagtcgcaat
     1981 gatcatttgc gccaaacggc attacgcaac gcacataccc cggcgtcgtt gttaacgaca
     2041 ttgactgagt ctcaggatcg gtcgctggct attaataatc cacagctggc tgccgatgtg
     2101 aaaacggtgt ggctaaaaga ggatccatca ttacttttat ttgtcgataa acccgatctt
     2161 tcgcagttac gggatttagt gaaaaccggg gcaacgcgga aaattcgcaa tgaagcgcgt
     2221 caccggcttg aggaaaaaca atgacacaac gcccctggag caagttacag cgggagatct
     2281 atgacttact gacgccaacg atcaacctgc aaattcactg tacccgttat cccatgcgca
     2341 gccaaaatgg tggaagcacc gatttaccgc gttattggat cacgttggat aaaaacgtga
     2401 tctgggatta cccgaaagac tttattgcag gcaacggtgg agtgcgtaat tttcacggtg
     2461 aaacttgctg gtatccttac ctgacggata tttgttcaat ttctgattta ttgcgtgaat
     2521 atattgacac cccaaaagcg gagttgttaa caaaacaatt taccagcgat aaatgggggt
     2581 tagtgaatat tctgcgtgct gctgacaggc gaatagggat gaggcgatta gatcagttac
     2641 ggcgtaaaac gcataatatt gctgcgctaa aaaattattg cccgccgtag cgaataatta
     2701 tatgcctggt gtggcttcgt acgccggata agacgcggca ggcgtcgcat ccggcattaa
     2761 aggaaaatca gcaattaacg ttgtgcttcg ccacctaatc cttcaatcag gttttgaatt
     2821 aacgctgcca gttcaccagt catcaggatg aaatcggcat caaaacgctg ggcgaaatct
     2881 tcacggtcga tatcttcgtt ttgatcgcgc agctcgtcgc agaacttcag acgcttgagc
     2941 gaaccatcgt cgcacatcac aaactgaatg cgctgctgcc agtcgagcgc cagtttagtc
     3001 accacttttc cggcttcaat gtgattggtg atctcttcgc tggtcagatc ttgtttcttc
     3061 gcgcggatca ctccgccatc ttccagcaac gatttcagct cggcttcatc aagcagctgg
     3121 aagccctgtg ccgcactacc ggaacgtacc cattcggtca gcgtcagttc aatcgggttt
     3181 gccatgctca atggtacaac cggtaacgac cccaggcttt tacgcagcag tgccagcgta
     3241 tcttccgctt ttttggcact ggcgcagtcc accataatca aaccgttaac cgtgtcgatc
     3301 cacatcattg tctggctaaa acggctgaaa gcacgcggca acagagagtg cagcacttcg
     3361 tctttcagcg aatctttttc ggttttcttc agcttacgcg cctgttccgc ttccagtttg
     3421 gcgattttcg cttccagcgc ctgtttaatc accggagacg gtaggatttt ttcttctttg
     3481 cgcgcgcaga taacaatttg accattggca acgtgcgtta acgcatcgct gtgcgatccc
     3541 atcggaggaa cccagcccat cttcgccatg tcctggctgc cgcatggggt aaatgccatc
     3601 gaggctagct gtttttccat ctcttctgca cgcagcgaaa tctcgcggct aagacggtaa
     3661 accattaaat ttttgaacca cagcatgata atttccacgg ccttgtcgtt aaatttagcg
     3721 ggcatgataa cgaattgtcg gcggccttgc attgccaatc cggttgtccg tctctacgct
     3781 attgatattg aaaaaaataa ggagagtacc gtgcgtatag gtatcgattt aggcggcacc
     3841 aaaactgaag tgattgcact gggcgatgca ggggagcagt tgtaccgcca tcgtctgccc
     3901 acgccgcgtg atgattaccg gcagactatt gaaacgatcg ccacgttggt tgatatggcg
     3961 gagcaggcga cggggcagcg cggaacggta ggtatgggca ttcctggctc aatttcgcct
     4021 tacaccggtg tggtgaagaa tgccaattca acctggctca acggtcagcc attcgataaa
     4081 gacttaagcg cgaggttgca gcgggaagtg cggctggcaa atgacgccaa ctgtctggcg
     4141 gtttcagaag cagtagatgg cgcggcagcg ggagcgcaga cggtatttgc cgtgattatc
     4201 ggcacgggat gcggcgcggg cgtggcattc aatgggcggg cgcatatcgg cggcaatggc
     4261 acggcaggtg agtggggaca caatccgcta ccgtggatgg acgaagacga actgcgttat
     4321 cgcgaggaag tcccttgtta ttgcggtaaa caaggttgta ttgaaacctt tatttcgggc
     4381 acgggattcg cgacggatta tcgtcgtttg agcggacatg cgctgaaagg cagtgaaatt
     4441 atccgcctgg ttgaagaaag cgatccggta gcggaactgg cattgcgtcg ctacgagctg
     4501 cggctggcaa aatcgctggc acatgtcgtg aatattctcg atccggatgt gattgtcctg
     4561 gggggcggga tgagcaatgt agaccgttta tatcaaacgg ttccgcagtt gattaaacaa
     4621 tttgtcttcg gcggcgaatg tgaaacgccg gtgcgtaagg cgaagcacgg tgattccagc
     4681 ggcgtacgcg gcgctgcgtg gttatggcca caagagtaaa aaacccaggc aattggcgca
     4741 tcatgcctga tgcgacgctt gccgcgtctt atcaggccta caaaatgtgc cagaaccgcg
     4801 tagggcggat aaggcgttca cgccgcatcc ggcaataagc aacgcacacc gtgacagtaa
     4861 tctaccccag tggtttcgcc agcaccggac tatccgccgc ttgctggcgc ttatagcgac
     4921 catacagcag caacgatgac atcgcagcaa acgaaagcag ggcggcaggc agcgccacgt
     4981 aattatatgc cagccccagc gtcagcatca tacctccgca atatgcgccg acggcgctac
     5041 cgaggttaaa cgctatttgc ccacctgcgg cacctaataa ctctccgcct ttggcatttt
     5101 gcagtaacaa tatttgcaga ggtgctgaaa gggcaaataa tcccgcgcaa caaataaaag
     5161 caaaaataag cgacgttgtt ttcatgccgc cgcagaaaaa gagcatcagc agtgccagta
     5221 caattataaa gtcagtcact gctgcaatgc gcagtggtga ataacgtcct gaaatcctgc
     5281 cacttagcat atttcccagc accatcccta gcccaactaa catcataata aaggtcatcg
     5341 ccgtttccga aaaaccggaa ataaacatca tgtatggctt tacgtagctg aaccaggcaa
     5401 acacacctgc gttgccaaac atggtggcgg cgaaaattaa ccacggggcc gggctgcgca
     5461 aaaagtgaaa ttgttcgcgc agctttcctt tcgcctcgtc gcgaatatcc ggcacccaaa
     5521 aatagaccga tgccatcacc gcaatattaa aaacagcgat caataaaaag gtgtaacgcc
     5581 agctaaattc ctgacttaaa tacgttccca gcggaatgcc cagcaaattg gcgactgtca
     5641 tcccggaaac catccccgcc acggcggcgg tgacttttcc gggtttgata atttttgata
     5701 acacgatcgc tccgacgcca aaaaatgcgc catgcggaaa gccggaaacc aggcgaccaa
     5761 tggcaagcat caggtacgac gaagagagcg tgaacatggc gttgccaatg acgcacaacg
     5821 ccaccagaaa caacaagata tgtttgagtg agtagcggct ggaaaagagt gcgattattg
     5881 gcgcaccgac caccaccccc agcgcataat acgagatcat atgcccggca gcaggaatcg
     5941 aaattcctac gttatgcgcc agctccgtga gcacgcccat aatgccaaat tcggccatcc
     6001 ccaaaccaaa cgtgcccaga gccaaagata aaatgacttt tttcatacca ccatcacaac
     6061 taccagaacg gctaccaatc accgtagcca ataaagtgat agcttgcagg ataacgacca
     6121 gcaaggccaa gagataattc gcccctctgt attcattatc ctgctgaata gttatttcac
     6181 tgcaaacgta ctttccagtt tgctgtagcc caggccgttg atcttcttca ctttgatctg
     6241 cacaggaata cgctctttca tcgcttcaac gtggctaatc acaccgatgg ttttgccact
     6301 ggcgttcagg gcatccagcg catcaagggc ggtatccagc gtttcgctat ccagcgtgcc
     6361 aaaaccttca tcaaggaaca gcgagtcaat acgtgtttta tggctgacca gatccgaaag
     6421 cgccagcgcc agcgccagac taacgaggaa actttcgccg ccggaaaggg tacgggtatc
     6481 gcgtaccgca tctgcctgcc aggtatcaac aacctcgact tccagcgcct cgctggcttt
     6541 gcgctgtaac agatagcgcc cgtgcagccg ggtaagttgc tgattagcga gatggactaa
     6601 attatccagc gtcagcccct gggcaaactt gcggaattta tcgccctctt tggaacctat
     6661 tagcgaattc agatatcccc agtcctcaac ctgctgcgtc atttgagcaa tttgctgcag
     6721 taaggtttgt tgttgctgac ggttatctgc atcctgcttc agctgctggc gaatctcgcc
     6781 ttgactcgtg gtgttttcac gcaacttttg gtgagtttgc gctaactctt gctgaatctg
     6841 ctccaccgtc acagtgagag ccaacccgtc aggtcggtgt tgttgatgct gtgccagcgt
     6901 ttctgctgtc tgagtgacca gcgtttgcgc ctgacggcgc tggttttcca gattctgctt
     6961 gagctgttcc agctgcgtta gtgtttgttc atccattagc gccgcaagga acgcctgctg
     7021 atcgtcaaag acgctggcct gtagcgcggt gtcaaactgc gcctgggctt tttgcagact
     7081 ttgcgccgcc agaacatcct gttgctgtaa cgtctgctgc tggctgtgta atgcgagaca
     7141 ttgttcatga acctgccgcc agttatccag cgccacagtt tcttcgctgt gcgggagatc
     7201 atcactttgc ggcaacgttt ccagaatcgg cgtcagctgc tgaatacggt tttgcagcgc
     7261 ggttaattcg ttctggcgtt gctgccagct ctgcgcttct tgctgacgtg tcgccaacca
     7321 gctctcttct tcatcttcct gtggcaatgt cagtgcataa cccgccaatg ccgttaagag
     7381 ctgctgctgg cgttgttcaa tttgctgttg atactggata atttgctgat tatgcgcggc
     7441 aatctgccct tgtaattcat gtcgttggct gagtaaccgc agctggcgtt cgtgctcatc
     7501 ttgtgcatcc agccacggtt gaatatcgtc ctgtggctgc aaggtgatat tgaggctggc
     7561 cgtgacggct tgccattgtt gagtaagtgc ttgctcatct tgtcggaggc tttgcgcttc
     7621 gttttcatcg cgctgaagct gcttcgttaa tgcatccagt tgcccgcgca gcgccgcacc
     7681 ttcttcgccg agctttttaa cttcgttttc cagcgccagt aatcgagact gattaacgcc
     7741 aggctccagc gcctgatacg cctcgaccgc cggatggctg gtggaaccac aaagtggaca
     7801 aggctgaccc gcctgtaact gtgcacgttg agcttccagc gttttgatgc gcgcttcctg
     7861 ctcgcaaatg gttttcacat cggcaagttg ctgcgtcttt tctttataac gctggcgcat
     7921 ttcgttaagt gcggcattac gttgcgtctg ttccagagtg acattctgga tagtgaccat
     7981 taactgcgcc agacgttttt gttggggaac aatctgccca tgcagcgcga ccaggcgctg
     8041 acgcagtggg cgttgctcag catgttgcgc cagggcggta gcaacttcat cggcggttaa
     8101 catcaacgtg atcgccgcaa gcgcattaag tttttgctca gcatgggtta actgttgctg
     8161 ccattgccgc agatgctcgc gatcgctggt ttgttgggag aactgcgcac gccaacccgc
     8221 cagttcgttg ttccactgac ggaagcggtc gtgttcctgt aaccaggtat tcaggctttg
     8281 ttgctgctgc tgtaattctg ctgactgctt cgccgcgtgg tggcgaatgc tcgcgcgaag
     8341 cgccattgtg ttctgtaagc gagtatttac ttcttcaatc tgctggcgag tatgcgccag
     8401 cgccgcgctg tgttctgcga tgcgttccca gtgcggacga agatttcgtg ccggttgtgc
     8461 cagactgagc gccgccagtt gaggttgcgc tttttcttct tcggctaacg cctgttgcaa
     8521 ggcctgctga cggcggctgg cttcttgctg caattcgtcc agacgcgtta accagtttag
     8581 tgattgttgt tcttgctgct gcgcggtaag taactgtttt tcttcgtcag taagtacctg
     8641 caaactcgct gtcagcgatt gcacttgttc cggcgtgagc aacgcgacgc cgctggcctg
     8701 cgcttgcagc ttctccagct ctgtgcgggc cgatttgtgc tgctcaaaaa ccatcgccga
     8761 gatttgcccg tagatttcag tgccggttaa ctcctcgagc aattccgcgc gttctttggg
     8821 tttggcattc aggaaggcag caaattgccc ctgcgaaagc agcatcgaac gggtgaagcg
     8881 cccgtaatcc agcccggtta acgtcgctgt cagttccagc ttatctttca ctttgtcggc
     8941 gagaattttg ccgtcggcgc agcgcgccag ctctacgcgt ggcacctgca aattaccgtc
     9001 gggttggtta cgcgcccgat tctggctcca gaacgcgcga tacgcttcac ctttcacttc
     9061 aaactccacc tccgccagac attcagcggt atcgcgggtc atgagatcat tttgcgattg
     9121 tgaaacgtta gagagacgcg gagtttcgtg atacagcgcc agacaaatgg cgtccagcag
     9181 ggtggttttc cccgcaccgg tcgggccggt aatggcaaac agcccgttgc tggcgaacgg
     9241 ctcgcgggtg aaatcaatct tccattcgcc ttttaatgag ttcaggtttt tcaggcgcag
     9301 gctgagaatt ttcatgcttc gtgttctccg gcgagggtat gcaacgtcgt ggtgaaaaga
     9361 tgctgcagac gttgctgctg cgattcatcc agttcttcca gtgccagacg gcgattgaac
     9421 acctcttcga cgctgagttc gctgagggtt tcacgctgtt ggctggctaa cacacgctcg
     9481 cgctgttcac gactccgacg taccagcaat acttcaacag gcaatgattc ggttaatgcc
     9541 tggattttgc gctgaatatc atgcagatac tcatcagtag tgatttcgat atccagccag
     9601 acaggtggct cctgcgatac atcgcgccac tgttccagct gtgcggtaat cgacgccaga
     9661 tcgcctttca gcactgccat gggttgcgtt accggtacgt tcaggttttc cacgctctct
     9721 aatttgccgt ttgaaaatgt caccagatgg acatatttac tcttaccgca ttcatcaaaa
     9781 ctcagtggaa tgggggagcc gcaatagcga acatgttcca tgccgccaat aatctgtgcg
     9841 cggtgaatat gcccgagcgc gatgtagtcg gctggtggaa agttttgtgc cggaaacgcg
     9901 tccagcgtgc caatataaat gtcacgcacg gcgtcacttt tactggcccc cacggtcgtt
     9961 aaatgtcccg tggcgatgat gggcagaggc tgatcgccgc gcagtttgca ggcatcggca
    10021 tagtgttgtt ggtaataatc ggtaatcgct gccagtaaat gctgctgttt ttcaataccg
    10081 ttaagccccg cctggctggt aataatgtca cgcggacgta aaaacggaat ggggcacagc
    10141 actgcgcctg gcgtcccgtc gcgacgaggc aagatttgcg gcgcatgtcc ggcgctggcg
    10201 accacggtag tattgaggaa cgccatgata tcgcgcgatt cattcagcgt ggcgaccgag
    10261 tcatggtttc ctgccagtac caccagatga cagccagttt gctgtaaatt gacaacaaaa
    10321 cggttgtata acgtgcgggc gtaactgggc ggcgagccgg tatcgaaaac atcaccggca
    10381 acaataatcg catccacctg atgggtttgt gctgtctcca gcagccagtc aagaaaagcc
    10441 tgatgttcag cttcgcggct tttactgtag aagttctggc cgagatgcca gtctgaggtg
    10501 tgaaggatgc gcataacggt tccctggcga aaaagcatgg gcgcgattat acccaaacag
    10561 atgtgccatt tgcttttttc tgcgccacgg aaatcaataa cctgaagata tgtgcgacga
    10621 gcttttcata aatctgtcat aaatctgacg cataatgacg tcgcattaat gatcgcaacc
    10681 tatttattac aacagggcaa atcatggcga gacgtattct ggtcgtagaa gatgaagctc
    10741 caattcgcga aatggtctgc ttcgtgctcg aacaaaatgg ctttcagccg gtcgaagcgg
    10801 aagattatga cagtgctgtg aatcaactga atgaaccctg gccggattta attctccttg
    10861 actggatgtt acctggcggc tccggtatcc agttcatcaa acacctcaag cgcgagtcga
    10921 tgacccggga tattccagtg gtgatgttga ccgccagagg ggaagaagaa gatcgcgtgc
    10981 gcggccttga aaccggcgcg gatgactata tcaccaagcc gttttcgccg aaggagctgg
    11041 tggcgcgaat caaagcggta atgcgccgta tttcgccaat ggcggtggaa gaggtgattg
    11101 agatgcaggg attaagtctc gacccgacat ctcaccgagt gatggcgggc gaagagccgc
    11161 tggagatggg gccgacagaa tttaaactgc tgcacttttt tatgacgcat cctgagcgcg
    11221 tgtacagccg cgagcagctg ttaaaccacg tctggggaac taacgtgtat gtggaagacc
    11281 gcacggtcga tgtccacatt cgtcgcctgc gtaaagcact ggagcccggc gggcatgacc
    11341 gcatggtgca gaccgtgcgc ggtacaggat atcgtttttc aacccgcttt taacgccttg
    11401 ctcatcggac gcagagcagg gcttatgatt tcttaactgg agtatcttac gtgctggaac
    11461 ggctgtcgtg gaaaaggctg gtgctggagc tgctactttg ctgcctcccg gctttcatcc
    11521 tgggtgcatt ttttggttac ctgccctggt ttttgctggc atcggtaaca ggactgctta
    11581 tctggcattt ctggaattta ttgcgccttt catggtggct gtgggtggat cgcagtatga
    11641 ccccgccacc ggggcgtggt agctgggaac cgctactata cggcttacac cagatgcagc
    11701 tgcgaaataa aaaacgccgc cgtgaactgg gcaatctgat taaacgcttt cgtagcggcg
    11761 cggagtcgct gcccgacgcg gtggtgctga ccacggaaga gggcggtatt ttctggtgta
    11821 acggtctggc gcaacagatt cttggtttgc gctggccgga agataacggg cagaacatcc
    11881 ttaacctgct gcgttacccg gagtttacgc aatatttgaa aacgcgtgat ttttctcgcc
    11941 cgctcaatct ggtgctcaac accgggcggc atctggaaat tcgcgtcatg ccttataccc
    12001 acaaacagtt gctgatggtg gcgcgtgatg tcacgcaaat gcatcaactg gaaggggcgc
    12061 ggcgtaactt ttttgccaac gtgagccatg agttacgtac gccattgacc gtgttacagg
    12121 gttacctgga gatgatggat gagcagccgc tggaaggcgc ggtacgcgaa aaagcgttgc
    12181 acaccatgcg cgagcagacc cagcggatgg aagggctggt gaagcagttg ctaacgctgt
    12241 cgaaaattga agccgcaccg acgcaattgc tcaatgaaaa ggttgatgtg ccgatgatgc
    12301 tgcgcgttgt tgagcgcgag gctcagactc tgagtcagaa aaaacagaca tttaccttcg
    12361 agatagataa cggcctcaag gtgtctggca acgaagatca gctacgcagt gtgatttcga
    12421 acctggtcta taacgccgtg aatcatacgc cggaaggcac gcatatcacc gtacgctggc
    12481 agcgagtgcc gcacggtgcc gaatttagcg ttgaagataa cggaccgggc attgcaccgg
    12541 agcatattcc gcgcctgacc gagcgttttt atcgcgttga taaagcgcgt tcccggcaaa
    12601 ccggcggtag cggattaggg ttagcgatcg tgaaacatgc ggtgaatcat cacgaaagtc
    12661 gcctgaatat tgagagtaca gtaggaaaag gaacacgttt cagttttgtt atcccggaac
    12721 gtttaattgt caaaaacagc gattaatccg cctttgtcat cttttattgc cataagccag
    12781 ccgatgctgg cttattttct ttgcagtcaa aatacgggcg ttagatttta caacggttgg
    12841 tgattttttg ttcgcatgat tagccatgtc tttttcacgg aaatagtgtt ttatactggt
    12901 tggtgatttc ttatcgctat atacctctgg tttttagatc cctccttgct ttaaaacgtt
    12961 ataagcgttt aaattgcgct tcaggtgctg tcatactgac tgcattaacg cggtaaatcg
    13021 aaaaactatt cttcgccgcg cctggttggg agtatttccc gctaaaattg tttaaatata
    13081 ccgctgtatc atccccaggg attggcacaa aaatttaacg ttacaacacc acatccacag
    13141 gcagtatgat ttatgaccca tcaattaaga tcgcgcgata tcatcgctct gggctttatg
    13201 acatttgcgt tgttcgtcgg cgcaggtaac attattttcc ctccaatggt cggcttacag
    13261 gcaggcgaac acgtctggac tgcggcattc ggcttcctca ttactgccgt tggcctgccg
    13321 gtattaacgg tagtggcgct ggcaaaagtt ggcggcggtg ttgacagcct cagcacgcca
    13381 atcggtaaag tcgctggcgt actgctggca acggtttgtt acctggcggt ggggccgctt
    13441 ttcgctacgc cgcgtacagc taccgtttct tttgaagtgg ggattgcgcc gctgacgggt
    13501 gattccgcgc tgccgctgtt tatctacagc ctggtctatt tcgctatcgt tattctggtt
    13561 tcgctctatc cgggcaagct gctggatacc gtgggcaact tccttgcgcc gctgaaaatt
    13621 atcgcgctgg tcatcctgtc tgttgccgcg attgtctggc cggcgggttc tatcagcacg
    13681 gcgactgagg cttatcaaaa cgctgcgttt tctaacggct tcgtcaacgg ctatctgacc
    13741 atggatacgc tgggcgcaat ggtgtttggt atcgttattg ttaacgcggc gcgttctcgt
    13801 ggcgttaccg aagcgcgcct actgacccgt tataccgtct gggctggcct gatggcgggt
    13861 gttggtctga ctctgctgta cctggcgctg ttccgtctgg gttcagacag cgcgtcgctg
    13921 gtcgatcagt ctgcaaacgg cgctgctatt ctgcatgctt acgttcagca cacctttggc
    13981 ggcggcggta gcttcctgct ggcggcgtta atcttcatcg cctgcctggt aacggcagtt
    14041 ggcctgacct gtgcttgtgc agaattcttt gcccagtacg taccgctctc ttatcgtacg
    14101 ctggtgttta tcctcggcgg cttctcgatg gtggtttcta acctcggctt aagccagctg
    14161 atccagatct ccgtaccggt gctgaccgct atttatccgc cgtgtatcgc actggttgta
    14221 ttaagtttta cacgctcatg gtggcataat tcgtcccgcg tgattgctcc gccgatgttt
    14281 atcagcctgc tttttggtat tctcgacggg atcaaagcat ctgcattcag cgatatctta
    14341 ccgtcctggg cgcagcgttt accgctggcc gaacaaggtc tggcgtggtt aatgccaaca
    14401 gtggtgatgg tggttctggc cattatctgg gatcgcgcgg caggtcgtca ggtgacctcc
    14461 agcgctcact aaatcactga acatttgttt taaccacggg gctgcgatgc cccgtggttt
    14521 tttattgtgt tgatgggtta ggaattgatg gaaagtaaga acaagctaaa gcgtgggcta
    14581 agtacccgcc acatacgctt tatggcactg ggttcagcaa ttggcaccgg gctgttttac
    14641 ggttcggcag acgccatcaa aatggccggt ccgagcgtgt tgttggccta tattatcggt
    14701 ggtatcgcgg cgtatatcat tatgcgtgcg ctgggggaaa tgtcggtaca taacccggcc
    14761 gccagctctt tctcgcgtta tgcgcaggaa aacctcggcc cgctggcagg ttacattacc
    14821 ggctggacct actgctttga aatccttatt gtcgccatcg ccgatgtgac cgcttttggt
    14881 atctatatgg gtgtctggtt cccgacggtg ccgcactgga tttgggtact gagcgtggtg
    14941 ctgatcattt gcgccgtaaa cctgatgagc gtgaaggtat tcggtgagct ggaattctgg
    15001 ttctcgttct ttaaagtcgc caccatcatc atcatgattg tcgccggttt cggcatcatc
    15061 atctggggga ttggcaacgg cgggcaaccg accggtattc ataacctgtg gagcaacggc
    15121 ggcttcttca gtaacggctg gcttggcatg gtaatgtcgt tgcaaatggt gatgtttgct
    15181 tacggtggga tcgaaattat cgggattacc gccggtgaag cgaaagatcc tgagaaatcg
    15241 ataccgcgtg cgattaactc cgtgccgatg cgtattctgg tgttctacgt cggtacgctg
    15301 ttcgtcatta tgtctatcta cccgtggaat caggttggca ctgccggtag cccgttcgtg
    15361 ctgacgttcc agcatatggg cattaccttt gccgccagca ttcttaactt tgttgtgctg
    15421 actgcttcgc tgtcggcaat taacagtgat gtatttggcg taggccgtat gctccacggt
    15481 atggcagagc agggcagcgc gccgaaaatt ttcagcaaaa cgtcgcgtcg cggtattccg
    15541 tgggttacgg tgctggtgat gactaccgcg ctgctgtttg cggtgtatct gaactacatc
    15601 atgccggaaa acgtcttcct ggtgatcgct tcgctggcaa ccttcgccac ggtgtgggtg
    15661 tggattatga tcctgctgtc gcaaattgcc ttccgtcgcc gtttgccgcc agaagaagtt
    15721 aaggcgctga aatttaaagt gccgggtggg gtagcaacga ccatcggcgg gctgattttc
    15781 ctgctcttta ttatcgggtt gattggttat cacccggata cgcgtatctc gctgtatgtc
    15841 ggtttcgcgt ggattgttgt gctgttgatt ggctggatgt ttaagcgtcg ccacgatcgt
    15901 cagctggctg aaaaccaata atacttgcgt tctgtaagcc ggttcaggag gaaatcctga
    15961 tccggctatt ccgaaagtta tccgcccccg tcctcctccc ccaaatatcc ttcagatgat
    16021 gagtgatcct gcattaggct atggcaaggt gatcagattt tcatcacagg ggaattatga
    16081 tgttaaatgc atggcacctg ccggtgcccc catttgttaa acaaagcaaa gatcaactgc
    16141 tcattacact gtggctgacg ggcgaagacc caccgcagcg cattatgctg cgtacagaac
    16201 acgataacga agaaatgtca gtaccaatgc ataagcagcg cagtcagccg cagccaggcg
    16261 tcaccgcatg gcgtgcggcg attgatctct ccagcggaca accccggcgg cgttacagtt
    16321 tcaaactgct gtggcacgat cgccagcgtt ggtttacacc gcagggcttc agccgaatgc
    16381 cgccggcacg actggagcag tttgccgtcg atgtaccgga tatcggccca caatgggctg
    16441 cggatcagat tttttatcag atcttccctg atcgttttgc gcgtagtctt cctcgtgaag
    16501 ctgaacagga tcatgtctat taccatcatg cagccggaca agagatcatc ttgcgtgact
    16561 gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc gatctggacg
    16621 ggataagcga aaaactgccg tatctgaaaa agcttggcgt gacggcgctg tatctcaatc
    16681 cggtgtttaa agctcccagc gtacataaat acgataccga ggattatcgc catgtcgatc
    16741 cgcagtttgg cggtgatggg gcgttgctgc gcttacgcca caatacgcag cagctgggaa
    16801 tgcggctggt gctggatggc gtgtttaacc acagtggcga ttcccatgcc tggtttgaca
    16861 ggcacaatcg tggcacgggg ggagcttgtc acaaccccga atcgccctgg cgcgactggt
    16921 actcgtttag tgatgatggc acggcgctcg actggcttgg ctatgccagc ttgccgaagc
    16981 tggattatca gtcggaaagt ctggtgaatg aaatttatcg cggggaagac agtattgtcc
    17041 gccactggct gaaagcgccg tggaatatgg acggctggcg gctggatgtg gtgcatatgc
    17101 tgggggaagc gggtggggcg cgcaataatt tgcagcacgt tgctgggatc accgaagcgg
    17161 cgaaagaaac ccagccggaa gcgtatatag ttggagagca ttttggcgat gcacggcaat
    17221 ggttacaggc cgatgtggaa gatgccgcca tgaactatcg cggcttcaca ttcccgttgt
    17281 ggggatttct tgccaatacc gacatctctt acgatccgca gcaaattgac gctcaaacct
    17341 gtatggcctg gatggataat taccgcgcag ggctttctca tcaacagcaa ttacgtatgt
    17401 ttaatcagct cgacagccac gatactgcgc gatttaaaac gctgctcggt cgggaggttg
    17461 cgcgcctgcc cctggcagtg gtctggctgt tcacctggcc tggtgtaccg tgcatttatt
    17521 acggtgatga agtgggactg gatggcaaaa atgatccgtt ttgccgtaag ccgttcccct
    17581 ggcaggtgga aaaacaggat atggcgttat tcgcgttgta ccagcgaatg attgcgctgc
    17641 gtaagaaaag tcaggcgctg cgtcgtggcg gctgtcaggt gctgtatgcg gaagataacg
    17701 tggtggtatt tgtccgcgtg ctgaatcagc agcgtgtact ggtggcaatt aaccgtggcg
    17761 aagcctgtga agtggtgcta cccgcgtcac cgtttcttaa tgtcgtgcaa tggcaacgca
    17821 aagaaggcca tgggcaactg acagacggga ttctggcttt gcctgccatt tcggctacgg
    17881 tgtggatgaa ctaaagcatc tgttttattt gttacgtcgt tagtcaccag ccctgacact
    17941 acgcagcgca aaaaagctgc tcaatgtcag ggctgaattt gttaaatatt tgtcccgatc
    18001 gatatgagcg atttttggta ttgtttgccc atgatgttat gtacaacatg ctccaggata
    18061 accctgtagt ggagcaaaga tatgacaatt tcaaattcga atttattgaa accgtttatt
    18121 actggtactg agttgccacc ttttaaagag gatgtctttt ctaaaacaga agaacaattt
    18181 aaaaaaagca ccta
//
LOCUS       DAZFKY010000094        18109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFKY010000094 DAZFKY010000000
VERSION     DAZFKY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            263..931
                     /locus_tag="ACHKCL_002802"
     CDS             263..931
                     /locus_tag="ACHKCL_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5763741.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            975..5741
                     /gene="ehaG"
                     /locus_tag="ACHKCL_002803"
     CDS             975..5741
                     /gene="ehaG"
                     /locus_tag="ACHKCL_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO5763742.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6109..7764
                     /gene="lldP"
                     /locus_tag="ACHKCL_002804"
     CDS             6109..7764
                     /gene="lldP"
                     /locus_tag="ACHKCL_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5763743.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7764..8540
                     /gene="lldR"
                     /locus_tag="ACHKCL_002805"
     CDS             7764..8540
                     /gene="lldR"
                     /locus_tag="ACHKCL_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5763744.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8537..9727
                     /gene="lldD"
                     /locus_tag="ACHKCL_002806"
     CDS             8537..9727
                     /gene="lldD"
                     /locus_tag="ACHKCL_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5763745.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9913..10386
                     /gene="trmL"
                     /locus_tag="ACHKCL_002807"
     CDS             9913..10386
                     /gene="trmL"
                     /locus_tag="ACHKCL_002807"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5763746.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10439..11260)
                     /gene="cysE"
                     /locus_tag="ACHKCL_002808"
     CDS             complement(10439..11260)
                     /gene="cysE"
                     /locus_tag="ACHKCL_002808"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5763747.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11340..12359)
                     /gene="gpsA"
                     /locus_tag="ACHKCL_002809"
     CDS             complement(11340..12359)
                     /gene="gpsA"
                     /locus_tag="ACHKCL_002809"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5763748.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12359..12826)
                     /gene="secB"
                     /locus_tag="ACHKCL_002810"
     CDS             complement(12359..12826)
                     /gene="secB"
                     /locus_tag="ACHKCL_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5763749.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12889..13140)
                     /gene="grxC"
                     /locus_tag="ACHKCL_002811"
     CDS             complement(12889..13140)
                     /gene="grxC"
                     /locus_tag="ACHKCL_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5763750.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13282..13713)
                     /gene="yibN"
                     /locus_tag="ACHKCL_002812"
     CDS             complement(13282..13713)
                     /gene="yibN"
                     /locus_tag="ACHKCL_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5763751.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13958..15502
                     /gene="gpmM"
                     /locus_tag="ACHKCL_002813"
                     /gene_synonym="pgmI"
     CDS             13958..15502
                     /gene="gpmM"
                     /locus_tag="ACHKCL_002813"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5763752.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15536..16795
                     /gene="envC"
                     /locus_tag="ACHKCL_002814"
     CDS             15536..16795
                     /gene="envC"
                     /locus_tag="ACHKCL_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5763753.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16799..17758
                     /gene="yibQ"
                     /locus_tag="ACHKCL_002815"
     CDS             16799..17758
                     /gene="yibQ"
                     /locus_tag="ACHKCL_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5763754.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17745..18109)
                     /gene="waaH"
                     /locus_tag="ACHKCL_002816"
                     /pseudo
     CDS             complement(17745..18109)
                     /gene="waaH"
                     /locus_tag="ACHKCL_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4558 a         4663 c         4712 g         4176 t
ORIGIN      
        1 tcccagacat ggtttctttc atatttgaca tttattaata atcatcatta gaatccacga
       61 aaataatata ttcatacaaa cacagacatc ttttgaaacg aatgatacat ttttataatg
      121 aatgcttaaa acaatattta atcgacacac atcactgtca ttaatgtaaa gcaacataga
      181 tcaccaataa tacaatggac aactctaccg gcattgatcg ctgggcggag gaaataagga
      241 tttttacatg aactagaaga agattttttt cagtgctgtc actgtttcag ttctctgcgc
      301 attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttat tgaaacaaca
      361 agaagaacat aacaataaaa ttgatctact tgaaaaacaa caagcacagc taaagagtca
      421 actggaaact attcaaaaac aacaaactgg cataataagc agtacaaaga ctttaactca
      481 cgtaattaaa tccgttaaag atcaacaaaa cacttttatt tttaccgagt ttaacccagc
      541 aaaaacgaag tatttcatcc tgaacaatgg ttcggtggct ttagcgggtc gagtgttatc
      601 cattgacgcc acagagaatg gcagtgttat tcatatttca ctggtcaact tattaagtac
      661 acctatctca aatattggtt ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa
      721 agagtttgcc cggtggcaac aattactttt caacacatca atgaaatcca cattgaaatt
      781 attaccaggt caatggcaag acattaattt gaccctgaag ggtgtatcgc ccaataacct
      841 gggatatctg aagttagcca tcaacatgga aaatattcag ttcgacaatc ttccctctgc
      901 tgaaaatcgg cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat aacaaggaat
      961 agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt
     1021 atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt
     1081 taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg
     1141 ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg
     1201 ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg
     1261 tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg
     1321 cgattggtgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta
     1381 tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt
     1441 caaaagcatt gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa
     1501 acgccattgc cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg
     1561 acaccgccaa tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg
     1621 aagaaaacgc tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca actgctattg
     1681 gcaataatgc gaaggcaaaa gggactaata gcatggcaat ggggttcgga agccttgccg
     1741 ataaagtcaa tactatcgca ttaggaaatg gcagccaggc tctggcagat aatgcaatcg
     1801 ccataggcca gggcaacaaa gctgatggcg tggatgccat cgctctgggt aatggtagcc
     1861 agtcgagagg cttaaacacc attgccttag gcacagccag taatgcaact ggtgataaga
     1921 gtcttgcgct tggtagtaat agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag
     1981 attccattgc ggatttagac aataccgtct ctgtcggcaa tagttcatta aaacgcaaga
     2041 tcgttaatgt gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt aatggttcac
     2101 agctttatgc cattagcgac tcggtagcaa aaaggcttgg aggaggggct gcagtagatg
     2161 ttgatgacgg tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg
     2221 taggggctgc gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca
     2281 aatacagcgc tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg
     2341 atggcacgat ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca
     2401 atgacgatgt cgaagccaac accgccaata tcgctactaa taccagcaac attgccacga
     2461 atacggcaaa tattgccacc aataccacca atatcaccaa cctgacggat tccgttggtg
     2521 accttcaggc tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg
     2581 gccaggatac caccagcaaa atcaccaacg ttaaagatgc cgacctgacg gctgacagca
     2641 ctgatgctgt taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca
     2701 ccaatatcgc caataacact tccaatattg ccactaacac caccaacatc tctaacctga
     2761 ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat
     2821 tcacggcagc tcatggcacc gagaccacca gcaaaatcac caacgttaaa gatggcgacc
     2881 tgacgactgg cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg
     2941 tggcgacgaa taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga
     3001 cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg
     3061 cagctcatgg caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg
     3121 caaccagttc cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca
     3181 cctacttcgg cggcaatgct tctgtgaata ctgacggtgt gtttaccggt ccaacctaca
     3241 aaatcggtga aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat
     3301 ttagcacgtc tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca
     3361 aacacggtac taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag
     3421 actccagttc tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg
     3481 atgcgctggg gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca
     3541 ccattgctaa tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc
     3601 ttgacgacgc tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc
     3661 acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg
     3721 ccagcagcga cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg
     3781 cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca
     3841 ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc
     3901 tgctgtggga tgagactgcc aatggcggtg ctggagccta caatgccagc catgacggta
     3961 aagccagcat catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag
     4021 tgaacggttc tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca
     4081 atcagctcgc tggtaacacc gacgcaacct atatccaaga aaacggtgcg ggtattaact
     4141 atgtgcgtac taacgacgac ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg
     4201 ctacagctat aggttataac tctgtcgcca aaggcgatag cagcgtagct attggtcagg
     4261 gcagctacag cgacgttgat acgggtatcg ccctgggtag cagctctgtt tccagccgag
     4321 tgattgccaa aggctcccgt gacaccagca taacggaaaa tggcgttgtt attggttacg
     4381 acaccacgga tggcgaactg ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc
     4441 aaatcatcaa cgtagccgat ggttccgaag cccatgacgc cgttacggtt cgtcaattgc
     4501 agaatgcgat tggtgcggtc gcaaccacgc cgactaaata cttccacgct aattcaacgg
     4561 aagaagattc actggcagtg ggaactgact cgctggcaat gggtgcgaaa accatcgtga
     4621 atggcgataa aggtattggt atcggttatg gtgcctacgt ggacgcgaat gcacttaacg
     4681 gcattgccat tggtagcaat gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg
     4741 gttctacgac cactcgtggc gctcaaacca attataccgc ctacaacatg gacgcaccgc
     4801 agaactctgt cggtgaattc tcagtcggta gtgcggatgg tcaacgtcag atcactaacg
     4861 tcgcagcagg ttcggctgat accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg
     4921 cgcaggtttc ccagaatacc cagagcatta ctaacctgga taatcgggta acgaatcttg
     4981 attcacgcgt caccaatatc gaaaacggta ttggcgatat cgtcaccacc ggtagcacca
     5041 agtacttcaa gaccaatacc gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg
     5101 cgattggttc cggctccatt gctgccgctg acaacagcgt cgctctgggt acagggtctg
     5161 tggcaaccga agaaaatacg atctctgtag gttcctctac taaccaacgt cgtatcacca
     5221 acgtagctgc aggtaaaaat gctaccgatg ctgttaacgt ggcacagttg aagtcttccg
     5281 aagctggcgg tgtacgttac gacaccaaag ctgatggttc tatcgactat agcaatatca
     5341 ccctcggtgg cggcaacggc ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca
     5401 acaacgacgt ggtgaattac gcgcagttga agcaaagcgt gcaggaaacg aagcaataca
     5461 ccgatcagcg aatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg
     5521 gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca ggcttacact ccaggtgcca
     5581 gcatggcctc tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat
     5641 cgatggtgag cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg
     5701 gtgaatactc cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt
     5761 gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct
     5821 ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt taccagacat
     5881 ctccccccac aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt
     5941 ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac cattcattgt
     6001 cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat
     6061 gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat gaatctctgg
     6121 caacaaaact acgatcccgc cgggaatatc tggctttcca gtttgatagc atcgcttccc
     6181 atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca
     6241 tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac
     6301 gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt
     6361 attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc
     6421 tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt
     6481 ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca
     6541 ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac
     6601 accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca
     6661 ggcatcgaca gctttgagat tggccagatg gtggggcggc agctaccgtt tatgaccatt
     6721 atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa ggagacgtgg
     6781 cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag ctctaacttc
     6841 attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg
     6901 ttcctcaaac gctggcagcc agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag
     6961 gttgatatga cgctagccca caccggttac actgcgggtc aggtgttacg tgcctggaca
     7021 ccgttcctgt tcctgacagc taccgtaaca ctgtggagta tcccgccgtt taaagccctg
     7081 ttcgcatcgg gtggcgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat
     7141 aaactggttg cccgtatgcc gccagtggtc agcgaggcta cagcctatgc cgccgtgttt
     7201 aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt
     7261 gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa
     7321 ctggctctac ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat
     7381 tccggactgt catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc
     7441 ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac
     7501 gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg
     7561 ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc
     7621 gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact
     7681 gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat
     7741 gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg
     7801 ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg
     7861 ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg
     7921 caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct
     7981 ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg
     8041 atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg
     8101 catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg
     8161 aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg
     8221 cgattgccga agcctcacat aacatcgtgc tgctgcaaac catacgcggt ttcttcgatg
     8281 tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt
     8341 cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg
     8401 gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg
     8461 atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt
     8521 cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg
     8581 cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct
     8641 gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat
     8701 gtctgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact
     8761 ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc
     8821 ggcggacgcg catggcattc cgtttactct ctcaacggtt tccgtttgcc cgattgaaga
     8881 agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg
     8941 ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac
     9001 cgtggatatg ccgacaccgg gagcgcgtta tcgtgatgcg cattctggga tgagcggccc
     9061 aaatgcggca atgcgccgct acttgcaagc ggtgacgcat ccgcaatggg cgtgggatgt
     9121 gggcctgaac ggtcgtccgc atgatttagg taatatttcg gcttacctcg gcaaaccgac
     9181 cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa
     9241 agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga
     9301 tccggaagat gcgcgcgatg cagtacgttt tggtgctgat gggattgtag tttctaacca
     9361 cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga
     9421 tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt
     9481 cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc
     9541 gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg agaaagagat
     9601 gaaagtggca atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct
     9661 ggtgcagggg ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc
     9721 ggcatagtcg tttgagcccc ctcaccctaa ccctctccct cagggagtgg ggaccgttcg
     9781 gcgctgtatg tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc
     9841 tccggggtga ggggaaaagg ccgcactgct caccaatttt ctgctatcct gcccccgcac
     9901 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     9961 aatatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga accgatggga
    10021 tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    10081 gttacgcgtc atcatgactt tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    10141 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa actatcagga tggcgattat
    10201 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    10261 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaacct gtccaatgcg
    10321 gtgtcagttg tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    10381 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    10441 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    10501 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
    10561 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    10621 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    10681 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    10741 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    10801 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    10861 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    10921 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    10981 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    11041 cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg
    11101 acagcttgtt cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg
    11161 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    11221 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    11281 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    11341 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc
    11401 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttctacgccg
    11461 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    11521 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    11581 ttacgcgact ggttgtcggt acaagtaagc acaagatcgc ccagccccgc catgcccata
    11641 aaggtggcag gatcggcacc cagcgccgcg ccaagacgcg acatttcagc cagcccacgg
    11701 gtgatcagtg ccgtacgcgc attcgcacca aaaccgatac cgtcggacat ccccgcgcca
    11761 atggcaatga cgtttttcac cgcaccgcca agctgcacgc caatgaaatc cggattgctg
    11821 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    11881 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    11941 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    12001 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    12061 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    12121 agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    12181 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    12241 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtgatggca
    12301 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    12361 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    12421 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    12481 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    12541 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    12601 acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt
    12661 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    12721 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    12781 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    12841 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    12901 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    12961 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    13021 cttggcggca ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag
    13081 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    13141 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    13201 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    13261 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaaa ggcaagtttt cgccagccca
    13321 gccagcgacg ccttctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagtgcgtt
    13381 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg
    13441 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    13501 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    13561 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    13621 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    13681 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    13741 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    13801 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    13861 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    13921 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    13981 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    14041 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    14101 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    14161 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    14221 caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa
    14281 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    14341 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    14401 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    14461 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    14521 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    14581 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    14641 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    14701 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    14761 cgaaatcact cgtgcattcg taaacgcaga tttcgacggc ttcgcgcgta agaaagtggt
    14821 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    14881 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    14941 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    15001 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    15061 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    15121 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    15181 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    15241 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    15301 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    15361 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    15421 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    15481 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    15541 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    15601 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    15661 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    15721 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    15781 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    15841 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    15901 tgccgcattc cgtcagggcg agcataccgg tattcagctg attctcagcg gtgaagaaag
    15961 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    16021 cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga
    16081 gaaacagagc gagcaacaaa cgctgttata tgagcagcgc gcccaacagg cgaaactgac
    16141 tcaggcgctg aacgagcgta aaaagacgct ggcagggctg gagtcttcca tccagcaagg
    16201 tcagcaacag ttgagcgagc tgcgcgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    16261 tgcggaagcc gcggcgaaag cgcgtgcaga acgagaagca cgtgaggccc aggcggttcg
    16321 cgaccgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    16381 atcgctgatg tcccgaactg gtggcctggg ggcgccgcgt ggtcaggcat tctggccggt
    16441 tcgcgggccg acgctgcatc gctatggtga acagctacag ggcgaactac gctggaaagg
    16501 aatggttatc ggtgcctctg aaggtactga agttaaagcg attgccgatg gtcgggtgat
    16561 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    16621 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    16681 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    16741 cgaaattcgc cgccaaggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    16801 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    16861 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    16921 ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    16981 cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc atctcccgat
    17041 ggcaccactg agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga
    17101 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    17161 caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    17221 actggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    17281 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    17341 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    17401 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    17461 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    17521 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    17581 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    17641 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    17701 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    17761 gcgctacgcg taatgcgatg gtgcgaccgc gtttttttcg cgccattgcc ataagcggaa
    17821 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    17881 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    17941 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    18001 ataaatcata attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    18061 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaac
//
LOCUS       DAZFKY010000095        17870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFKY010000095 DAZFKY010000000
VERSION     DAZFKY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHKCL_002817"
     CDS             complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHKCL_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5763755.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            370..1086
                     /gene="arcA"
                     /locus_tag="ACHKCL_002818"
     CDS             370..1086
                     /gene="arcA"
                     /locus_tag="ACHKCL_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5763756.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHKCL_002819"
     CDS             complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHKCL_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5763757.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHKCL_002820"
     CDS             complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHKCL_002820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5763758.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHKCL_002821"
     CDS             complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHKCL_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5763759.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHKCL_002822"
     CDS             complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHKCL_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5763760.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5366..6235
                     /gene="robA"
                     /locus_tag="ACHKCL_002823"
     CDS             5366..6235
                     /gene="robA"
                     /locus_tag="ACHKCL_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5763761.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHKCL_002824"
     CDS             complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHKCL_002824"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5763762.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6931..7443
                     /gene="yjjX"
                     /locus_tag="ACHKCL_002825"
     CDS             6931..7443
                     /gene="yjjX"
                     /locus_tag="ACHKCL_002825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5763763.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7590..7916)
                     /gene="trpR"
                     /locus_tag="ACHKCL_002826"
     CDS             complement(7590..7916)
                     /gene="trpR"
                     /locus_tag="ACHKCL_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5763764.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8006..9943)
                     /gene="sltY"
                     /locus_tag="ACHKCL_002827"
     CDS             complement(8006..9943)
                     /gene="sltY"
                     /locus_tag="ACHKCL_002827"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5763765.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10154..11821
                     /gene="ettA"
                     /locus_tag="ACHKCL_002828"
     CDS             10154..11821
                     /gene="ettA"
                     /locus_tag="ACHKCL_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5763766.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11928..12095)
                     /gene="nadS"
                     /locus_tag="ACHKCL_002829"
     CDS             complement(11928..12095)
                     /gene="nadS"
                     /locus_tag="ACHKCL_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5763767.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12129..13361)
                     /gene="nadR"
                     /locus_tag="ACHKCL_002830"
     CDS             complement(12129..13361)
                     /gene="nadR"
                     /locus_tag="ACHKCL_002830"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5763768.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13382..14764)
                     /gene="radA"
                     /locus_tag="ACHKCL_002831"
     CDS             complement(13382..14764)
                     /gene="radA"
                     /locus_tag="ACHKCL_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5763769.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14813..15781)
                     /gene="serB"
                     /locus_tag="ACHKCL_002832"
     CDS             complement(14813..15781)
                     /gene="serB"
                     /locus_tag="ACHKCL_002832"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5763770.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15887..16528
                     /gene="ytjB"
                     /locus_tag="ACHKCL_002833"
                     /pseudo
     CDS             15887..16528
                     /gene="ytjB"
                     /locus_tag="ACHKCL_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            16559..17575
                     /gene="lplA"
                     /locus_tag="ACHKCL_002834"
     CDS             16559..17575
                     /gene="lplA"
                     /locus_tag="ACHKCL_002834"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5763771.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17607..17870
                     /locus_tag="ACHKCL_002835"
     CDS             17607..17870
                     /locus_tag="ACHKCL_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5763772.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         4256 a         4986 c         4504 g         4124 t
ORIGIN      
        1 gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt
       61 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc
      121 ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg
      181 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
      241 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
      301 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
      361 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
      421 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
      481 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
      541 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
      601 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
      661 gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg
      721 ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
      781 aagttcaatg gttgggaact ggacatcaac agccgttctt tgatcggccc tgatggcgag
      841 cagtacaagc tgccgcgcag tgagttccgc gccatgcttc acttctgtga aaacccaggc
      901 aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccgcac
      961 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     1021 acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg tgatctggaa
     1081 gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt
     1141 caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
     1201 ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat gccggccagc
     1261 gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt gaggagtccc
     1321 cacatcacac catccagcaa caacagcgcg agggtaaaca acatgctgtt gcgccaacct
     1381 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
     1441 catgccacgg taaaaccggt atgttcagaa agcgccagca agagcaaata aaacatcacc
     1501 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
     1561 aaaacaaaga acgccataaa agtcagcgca atcagcagaa tggcatactt agtcgcccgg
     1621 tcagttaatt ggtattgatc ggcgggcgtc gtcactgcga cgctaaacgc ggggaagttt
     1681 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
     1741 ctttgccatt gcgcctgaaa acctgactca ctaacttccc gtttggctgg tagaaaatca
     1801 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgccccgca
     1861 ggcaccacag aaagatcgcc ggtaccgctt aaattcagtg ccatattcag cttcaggttc
     1921 tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
     1981 ccgatgccgg gttcaatagt cagcgccgtt ccgttaactt caggcgcttt caccacacca
     2041 ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt gatatttggc
     2101 gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     2161 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
     2221 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
     2281 tcttcctgca ccgtataaag ctcggtcaca ggaatagcga taagaggccc aacgagtttt
     2341 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt
     2401 tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     2461 agggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca
     2521 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagccga
     2581 agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
     2641 cgggcgacct cactgacaaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
     2701 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg
     2761 gtatccagca ccttaagggc gacgtgttcc tgatccactt cggcgcttag cgttatgcga
     2821 ccgctcttgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgctccagt
     2881 aacgccggtt cagcagcaac gttaacctca gtaggcataa catgcaaagt gatgtttttt
     2941 tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca
     3001 gcagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttctacc
     3061 aatgcctgca tacgcgcatt ttgcgtcaga atgttgtcgg taaaacgagc caccacttcc
     3121 ggtggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
     3181 agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
     3241 cgcatacttt ccagcgcctg cgcaagctta cgcaactcgc tactgccgag ttcaggaaga
     3301 ggaacgggct tattgtcagt gacggaatcg gcatagcgag tgagccgggc aatggagcgg
     3361 ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccagcaa aatggcgctg
     3421 gcccataaaa ttcgccgctc gctgcgctta atgaccggag ccatcgccgc gttcggtttg
     3481 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
     3541 accgaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata ctgaccacgc
     3601 aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt atttgccgaa
     3661 tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt gcgcactttg
     3721 ttaatgccac cgatattagc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc
     3781 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
     3841 gttgcggtgt cgatcagcgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
     3901 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcaac
     3961 aaccgcatgc cgatacgcat tacaggctcc tcaggctgta tcccatgccg cgatgagtat
     4021 taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt ttaatgtggg
     4081 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgatatcc atcagttgct
     4141 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
     4201 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
     4261 aatgaccaat acggatgacg ggagacggcg tcgagaactt cttcacccga cgcagcaagg
     4321 tgcgcaccct agcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc
     4381 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
     4441 gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccactg atatcaggca
     4501 aaccaacgtc gagaatcatg acatcgggaa cctgctggcg agctttatcc agcaccggca
     4561 agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
     4621 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
     4681 gttattgtcg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
     4741 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca taaaagcgca
     4801 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
     4861 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatag
     4921 ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct gttttcgccc
     4981 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
     5041 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
     5101 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
     5161 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
     5221 gaacaaaatc tcaatacttt tatttccgtc aagacctaaa acatactcta ctaagaaaaa
     5281 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
     5341 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
     5401 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
     5461 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
     5521 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
     5581 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
     5641 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
     5701 tggtattcgc ccgccgctgc gtctgggtga attcactatg ccagagcata aatttgtcac
     5761 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
     5821 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
     5881 gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
     5941 cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
     6001 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
     6061 gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc caatgctcaa
     6121 cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag acgccaaagc
     6181 gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc gttaacgctg
     6241 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacgaccca
     6301 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
     6361 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag
     6421 tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
     6481 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
     6541 atcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc
     6601 gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat caaagatgat
     6661 gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct
     6721 actgatgata tgcgtaatgc caagctcttt ggcacgcgtc gccacctgca tcgcctgttg
     6781 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
     6841 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
     6901 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat
     6961 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
     7021 catattgcat ccattgccgt cgagagcggt gtaccggaac agccctttgg cagtgaggaa
     7081 acgcgcgctg gcgcacgaaa ccgggtagcc aatgcccgcc gtttacttcc agaggctgat
     7141 ttttgggtgg cgattgaagc tggcattgat ggcgacagca ctttcagttg ggtggtgatt
     7201 gaaaacacca cccagcgcgg cgaggcgcgt tcggcaactt tgccgttgcc tgcggtgata
     7261 ttggaaaaag tacgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt
     7321 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc
     7381 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa tgcggtgtat
     7441 taagcgcctg atgcgacgct gccgcgtctt atcatgccta ccaaacatat tgaaattacg
     7501 ggtatttgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
     7561 atgcgccacg tcttatcagg cctacaaaat caatcgcttt tcagcaacac ctcttccagc
     7621 cactggcgca gctccacggg cgcggctttc aggctgttag atccacgcgt aatcgtcgcg
     7681 atgcctgcgc cgagttcatt ttttaactca cgctggctca tttcgccgcg caacagctct
     7741 tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag catcaggttt
     7801 aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac aaaacgtaac
     7861 cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg ggccataata
     7921 tgtcgccatt gttagcgggg gaagcaaaat gcctcccccg gttgtactcg ctaaagagta
     7981 cgatagcata tcataaacgt gcggatcagt aacgacgtcc ccattccgtg gcgctcatca
     8041 acgtcggttt atcccccatg aaatagcggt agtaagcgtc ataagccagc acgttcttca
     8101 cataaccgcg cgtctcggag aacggaatac tctcgacaaa tgccacagcg tcgatacgcc
     8161 cggcgctgtt gccaagccag gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg
     8221 agaaaatacg gttattacca aattgctgat aaacatattg caggtaactg gtgccaatgt
     8281 tgatattcgt ttccggatcc agcaattgcc cagggctgct ataaccagga atagagaaca
     8341 tcttcaccgt atgggtcgcc gtgcccggca taatctgcat caggccgctg gcccctaccg
     8401 gtgatttcac tttcggattc caggcgctct cctgacgggc aatcgccatc gcatagcttt
     8461 gcgggatctc cttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc
     8521 gctcttccag atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta agatcccacc
     8581 attggttgtt gaaagcatac cgcgccagtt gagcctgctc tgtttttgac ttgctcttca
     8641 ccagattggc ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc aactcgcgca
     8701 cgcgcgccat ctccggcccc tgagtcaggg cgctgtcaac attctgcggc gctttatcaa
     8761 tcttcagctc atactcttcg ccgatgcgtt gtgcagcaac catcgggtag aaaccacgct
     8821 gttgcatgag ttgatgcaaa atctcttttg cttcagcttc acgtccgcgt tccagcaata
     8881 aatccgcctg ccagtaacgc cattcatctt tctctttcgc ttccataggc agacgcgcca
     8941 gccaggtatt caggccgcga cgatcgccag tgccaagcgc cattcgcaca cggcgttcaa
     9001 taagcgaagt agattgcgag cgcataatgg cgtcatcgcg ccatttcgcc tgctcgtcgg
     9061 tgacatcgtt gcccatcaaa cgccaggcga cgatatcgcg cagctcctga atctgatctt
     9121 cattaagctg ctgcgcctgg gcaagcgatg ggatcatcag ccgtgcattt tcagcatcct
     9181 gccgcgccac actggcaaac gccaccgccg ccatttgacg ggtaaaatcg gtcgctccgg
     9241 ttgtacgcgc gaaggtcagt accgtattgg ggttgttcgc cagtgaaatg attgccgagg
     9301 cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg ccagtgttac
     9361 ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt ttacctgacg
     9421 cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg gttagccaca
     9481 gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc gcatagtagt
     9541 aattacattg tgcttcggta gttccgggct tttccgggct aaaggctaac aggccacgcc
     9601 agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga gcgggaggaa
     9661 gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc atcaggtcat
     9721 cggtgatctg gcggtattcc aggtagggat aaagcggata atccttcagt ccaggcatca
     9781 tttgttcgac cacatccatt tgtcgattat cccaggcctg cttgatttgc gcgtaacgac
     9841 tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc agacagacac
     9901 cggcagccaa cagccgccag gtaacttgtt tggctttttc cacaagcgca tcctctaagt
     9961 gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca gtcattgccg
    10021 tttacgccac gttacggaca cttttttact tttactgcga gggtgatcga ccggctggga
    10081 ttaggcgggg aaaaaggcta cacttcgcct ttgaaaactc atcatcacga taaacataga
    10141 ggcgaagtcc aacgtggctc aattcgttta taccatgcat cgtgtcggca aagttgttcc
    10201 gccgaaacgt catattttga aaaacatctc tctgagtttc ttccctgggg caaaaattgg
    10261 tgtcctgggt ctgaacggcg cgggtaagtc caccctgctg cgcattatgg cgggcattga
    10321 taaagacatc gaaggtgaag cgcgtccgca gccagacatc aagattggtt atctgccgca
    10381 ggaaccgcag ctgaacccgg aacacaccgt acgtgagtcc attgaagaag cggtttcaga
    10441 agtggttaac gccctgaaac gcctggatga agtgtatgcg ctgtacgccg atccggatgc
    10501 cgattttgac aagctggccg ctgaacaagg ccgtctggaa gagatcattc aggctcacga
    10561 cggtcataat ctgaacgtac agctggagcg tgcggcggat gcgctacgtc tgccggactg
    10621 ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct
    10681 gctgctggaa aaaccagaca tgctgctgct cgacgaaccg accaaccacc tggatgccga
    10741 atccgtggcc tggctggaac gcttcctgca cgacttcgaa ggcaccgtgg tggcgattac
    10801 ccacgaccgt tacttcctcg ataacgttgc gggctggatc ctcgaacttg accgcggtga
    10861 aggtattccg tgggaaggta actactcctc ctggctggag cagaaagatc agcgtctggc
    10921 gcaggaagct tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt
    10981 acgtcaggga actaaaggcc gccagtcgaa aggtaaagca cgtctggcac gctttgaaga
    11041 actgaacagc accgaatatc agaaacgtaa cgaaaccaac gaactgttta ttccacctgg
    11101 accgcgtctg ggcgataaag tgctggaagt cagcaacctg cgtaaatcct atggcgatcg
    11161 tctgctgatt gatgacctga gcttctcgat cccgaaagga gcgatcgtcg ggatcatcgg
    11221 tccgaacggt gcgggtaaat cgaccctgtt ccgtatgatc tctggtcagg aacagccgga
    11281 cagcggcacc atcactttgg gtgaaacggt gaaactggcg tcggttgatc agttccgtga
    11341 ctcaatggat aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg atatcatgaa
    11401 gatcggcaac accgagatgc caagccgcgc ctacgttggc cgctttaact ttaaaggggt
    11461 tgatcagggt aaacgcgttg gtgaactctc cggtggtgag cgcggtcgtc tgcatctggc
    11521 gaagctgctg caggttggcg gcaacatgct gctgctcgac gaaccaacca acgacctgga
    11581 tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct gtgcgatggt
    11641 tatctcgcac gaccgttggt tcctcgaccg tatcgccacg cacattctgg attaccagga
    11701 tgaaggtaaa gttgagttct tcgaaggtaa ctttaccgag tacgaagagt acaagaaacg
    11761 cacgctgggc gcagacgcgc tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta
    11821 atgcgtaaaa tgccggatgc ggcgcgaacg ccttatccgg cctacaaaac cagcaatttc
    11881 aatacgttgt aaaactgtag acctgataag acgcggtaag cgtcgcatca ggcattgagc
    11941 accgaatgcc ggatgcggcg tgaacacctt ttccggtcta caacagcctt agcatcctca
    12001 atttcgtggc gggccacccg tgcaggtttt ttcaaacctt gtttaatctc gacagcctct
    12061 tccagagagg ttttcaactc atcaaaaaaa ctcattttat acctcgcctt tgagcagttt
    12121 catcacggtt atctctgctc ccccatcatc tcccgcacca gctccacgca gcgcaggaaa
    12181 cggctgtcgt aatcttcctc ttcaacccgc acaaattcga tattgttctc ttccagcatc
    12241 tccaccagca gattctggaa ctctttacga tccaccgaac tgccgaggct gcgtaaacca
    12301 tccaccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca
    12361 atcagcgcct gcacgaacgg atgttcccgc ccttcgtact ttttgcagaa cgcctgagtg
    12421 gtgacaaaat cggtatcgat aaatgccact ttattggcat atttcaccgc aaaatcaatg
    12481 tattgagcgt ggcccagcgc gattttatcg tagtcagaat actgcaatgc gatctcatca
    12541 ccgccgaggt gtgaaaagac ataatcgcgg ccatattccc acgcactggt ggtgttgaag
    12601 atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccgcc aaggatcgcc
    12661 acggtacgca caaaaaacgg cttcacttcg gtaggaatat attcccagta gcggaacggg
    12721 ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gtttcggatc gaccagcacc
    12781 gtctcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga ggtgtagatc
    12841 agatccggct ggatcccctt ttcagccata aactttttga taccgttgct ccacacatcc
    12901 cagccgtgcg gatacggctc catgccctct tcgttgaaag catgaatgcg aatatttttc
    12961 tgatatttaa aagtttgcaa taaccagcgc agacgatccg gcacggtcgg ctgctgcgac
    13021 atggcactgt cttcgaacaa cgcgcggtca cgggtatcgt caaaacccat aatgatatgc
    13081 agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccggt atgcagtggg
    13141 tagaacttac cgaatacgac accgatcgtt ttcttctgcc ggggaaactc aagccccaaa
    13201 aaacggtgca acgcctccag cttttgcgcg ctggggcttt tgattttggc attcagtaac
    13261 tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc
    13321 tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatttctc
    13381 attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca
    13441 tcccttccgg cgcttttttc ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt
    13501 gtttcgccgc ttcagagatt cgttcctgac cgctgggcac cgggcggatc tcccctgcca
    13561 gcccgacttc accaaacacc accagatcct gcggtagcgg tctgtcacgc aggctggaaa
    13621 ccatcgccag cagtaacgct aagtcggcgc tggtttcggt tacctttacg ccgccgacca
    13681 cgttcacaaa cacatcctga tcggccattt gcagaccacc gtgacggtgc aagacagcca
    13741 gcaggattgc cagacggttt tgttccagcc ccactgccac gcggcgcgga ttcgccatca
    13801 tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct tcccacacca
    13861 ccatcacgga gctgccggag gtcacttcat ctccgcgact taagaaaatt gccgaagggt
    13921 tgctgacttc acgcagcccc tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc
    13981 cgaagcggtt tttatggctg cgcaaggtgc gaaaacggga gtcggcatcg ccatctaaaa
    14041 gcaccgaaca gtcgatacag tgttccagca ctttcgggcc agccagcgaa ccatctttgg
    14101 ttacgtgccc caccatgaca atcgccacac cgcgcgtttt ggcgaagcgt gtcaaataag
    14161 ccgccgtttc acgcacctgc gccacgctac caggcgatga ctgtacatcc gccatatgca
    14221 tcacctggat cgagtcaatt accatcagct tcggttgctc ttcttcggca atcaggcaga
    14281 tctgttcgat gctggtttcc gacaacatat tgagattgtc agtcggcagg ccaaggcgat
    14341 gagcgcgcat tgccacctgt tgcagcgact cttcgccggt gacatacagc gttttcatct
    14401 gctgggccag tttgcacagc gtttgcagta gcagcgtgga tttccccgca ccagggttac
    14461 cgccaatcag aatggcactt cctggcacca cgccgccgcc tagtacgcgg tcgaactctt
    14521 taaatccggt ggaaaaacgc ggcagctctt caaggctgat atcggagagt ttctggactt
    14581 ttgccacccc ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc accgtaggcg
    14641 acgcagcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct
    14701 gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg
    14761 ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg gcgggcgagc aattacttct
    14821 gattcaggct gcctgagagg atgcagaata cccccatcag gtcagcgtga cggatggtga
    14881 cttccgcctt ttcattcact tttggcttgg catggtaggc aatccccagc cctgccgctt
    14941 tgatcatcgg caggtcattg gctccatcgc caatcgccac ggtctgcgcc agcgggattt
    15001 catactcctg cgcgaggcga gtcagagttt tcgctttgta ctgcgcgtct acgatgtcgc
    15061 cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggctg
    15121 tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg gaggcaatcg
    15181 ccactttcca gcccagcgtt tccagcttga gcaccagttg cgttaagcct ggcatcagcg
    15241 gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc
    15301 ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacttccg
    15361 ccaccatctc gcccgttccg gccagtttgg caatttcatc aatacattca atctggatgg
    15421 cggtggaatc catgtccatc accagcaaac ccggcgtgcg caggtgcggg atttttccca
    15481 gcggcgcgac atccagctgc gcttcgtggg ccaggcgtgt agcccgtgcg gtgagtgaac
    15541 ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatcgccg
    15601 cacccagttt gctctggtat tgggtcagac gctgtttatc cagcccacga ccatacagca
    15661 gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa
    15721 gaggcagacc cggccataaa gagacatctt caggcaggtc gcaccaggta atgttaggca
    15781 ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc
    15841 cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa
    15901 actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc
    15961 actgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta atccacagct
    16021 ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg
    16081 taccgactca ccggatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag
    16141 ccgtatcctc gacgcgggtg tgtatgacga acaaggcgat cttatcgcac gttctggcga
    16201 aagcgtcgaa gtgcgcgatc ggctggcgct cgacggtaaa aaagcaggcg gctattttaa
    16261 ccagcagatt gtcgagccaa ttgccgggaa aaacggaccg ctcggctatc tgcgcctgac
    16321 actcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt
    16381 acgcctgatg ttgctgctct cactggcaat cggcgtagtg ctgacccgca cgctgctaca
    16441 gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagca aaccggtgcc
    16501 ggaagaggaa gaaagcgaga aaaaagagtg ccccattact acaagaaagg aaatcgttat
    16561 gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtgga
    16621 agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc
    16681 cgacacggta gtaattggtc gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat
    16741 ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga
    16801 tctcggcaat acctgcttta cctttatggc tggcaagccg gagtacgata aaaccatctc
    16861 cacgtcgatt gtgctcaatg cactgaatgc gctcggcgtc agtgccgaag cgtccggacg
    16921 taacgatctg gtggtgaaaa ccgccgaagg cgaccgcaaa gtctcaggat cggcctatcg
    16981 cgaaaccaaa gatcgtggct tccaccacgg caccttgctg ctcaatgccg accttagccg
    17041 cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacttcagt
    17101 gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg atcccccatg agcaggtttg
    17161 cgaggccata accgaggcct ttttcgccca ttatggcgag cgcgtggaag cggaaatcat
    17221 ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc gccagagtag
    17281 ctgggagtgg aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttag
    17341 ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt
    17401 gtttaccgac agcctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg
    17461 cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga
    17521 ccaggaaaaa gagctacggg agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc
    17581 tgattaaaat atccaaggaa ggaaatatga tcgatctgga aaaccaggaa cgggagatta
    17641 tcaatttaat gttcagccag cgtatttcct ggctagccgc cgtgcgtatc aggcataaat
    17701 tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat taattctcta aaacagatcg
    17761 aaaagacgga acggcttagc tcaaacatca aaagcaaaat ggcagaaatt tatggttgtc
    17821 caccagagtt gctgatatgc ccttcctgga tgacggctga acataaatga
//
LOCUS       DAZFKY010000096        17681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFKY010000096 DAZFKY010000000
VERSION     DAZFKY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            72..590
                     /gene="yfiR"
                     /locus_tag="ACHKCL_002836"
     CDS             72..590
                     /gene="yfiR"
                     /locus_tag="ACHKCL_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5763773.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            580..1806
                     /gene="dgcN"
                     /locus_tag="ACHKCL_002837"
     CDS             580..1806
                     /gene="dgcN"
                     /locus_tag="ACHKCL_002837"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5763774.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1822..2304
                     /gene="yfiB"
                     /locus_tag="ACHKCL_002838"
     CDS             1822..2304
                     /gene="yfiB"
                     /locus_tag="ACHKCL_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5763775.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(2381..2728)
                     /gene="rplS"
                     /locus_tag="ACHKCL_002839"
     CDS             complement(2381..2728)
                     /gene="rplS"
                     /locus_tag="ACHKCL_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5763776.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2770..3537)
                     /gene="trmD"
                     /locus_tag="ACHKCL_002840"
     CDS             complement(2770..3537)
                     /gene="trmD"
                     /locus_tag="ACHKCL_002840"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5763777.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3568..4116)
                     /gene="rimM"
                     /locus_tag="ACHKCL_002841"
     CDS             complement(3568..4116)
                     /gene="rimM"
                     /locus_tag="ACHKCL_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5763778.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4135..4383)
                     /gene="rpsP"
                     /locus_tag="ACHKCL_002842"
     CDS             complement(4135..4383)
                     /gene="rpsP"
                     /locus_tag="ACHKCL_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5763779.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4520..5881)
                     /gene="ffh"
                     /locus_tag="ACHKCL_002843"
     CDS             complement(4520..5881)
                     /gene="ffh"
                     /locus_tag="ACHKCL_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5763780.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6048..6839
                     /gene="ypjD"
                     /locus_tag="ACHKCL_002844"
     CDS             6048..6839
                     /gene="ypjD"
                     /locus_tag="ACHKCL_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5763781.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6861..8147
                     /gene="yfjD"
                     /locus_tag="ACHKCL_002845"
     CDS             6861..8147
                     /gene="yfjD"
                     /locus_tag="ACHKCL_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5763782.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVPDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8202..8795)
                     /gene="grpE"
                     /locus_tag="ACHKCL_002846"
     CDS             complement(8202..8795)
                     /gene="grpE"
                     /locus_tag="ACHKCL_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5763783.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8918..9796
                     /gene="nadK"
                     /locus_tag="ACHKCL_002847"
     CDS             8918..9796
                     /gene="nadK"
                     /locus_tag="ACHKCL_002847"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5763784.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9882..11543
                     /gene="recN"
                     /locus_tag="ACHKCL_002848"
     CDS             9882..11543
                     /gene="recN"
                     /locus_tag="ACHKCL_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5763785.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11692..12033
                     /gene="bamE"
                     /locus_tag="ACHKCL_002849"
     CDS             11692..12033
                     /gene="bamE"
                     /locus_tag="ACHKCL_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5763786.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12095..12385)
                     /gene="yfjF"
                     /locus_tag="ACHKCL_002850"
     CDS             complement(12095..12385)
                     /gene="yfjF"
                     /locus_tag="ACHKCL_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5763787.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12375..12812)
                     /gene="ratA"
                     /locus_tag="ACHKCL_002851"
     CDS             complement(12375..12812)
                     /gene="ratA"
                     /locus_tag="ACHKCL_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5763788.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            12983..13465
                     /gene="smpB"
                     /locus_tag="ACHKCL_002852"
     CDS             12983..13465
                     /gene="smpB"
                     /locus_tag="ACHKCL_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5763789.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13680..14042
                     /gene="ssrA"
                     /locus_tag="ACHKCL_002853"
     tmRNA           13680..14042
                     /gene="ssrA"
                     /locus_tag="ACHKCL_002853"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14311..14559
                     /locus_tag="ACHKCL_002854"
     CDS             14311..14559
                     /locus_tag="ACHKCL_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5763790.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(14675..14827)
                     /locus_tag="ACHKCL_002855"
                     /pseudo
     CDS             complement(14675..14827)
                     /locus_tag="ACHKCL_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(15061..15651)
                     /gene="espM2"
                     /locus_tag="ACHKCL_002856"
     CDS             complement(15061..15651)
                     /gene="espM2"
                     /locus_tag="ACHKCL_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO5763791.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            15834..16484
                     /locus_tag="ACHKCL_002857"
     CDS             15834..16484
                     /locus_tag="ACHKCL_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5763792.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            16563..17621
                     /gene="espW"
                     /locus_tag="ACHKCL_002858"
     CDS             16563..17621
                     /gene="espW"
                     /locus_tag="ACHKCL_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO5763793.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNVRNQNKFPIDVKETVIKLQGQKFTCIEDFYKRYNETRLKANTNIQQEQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISSDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
BASE COUNT         4727 a         4459 c         3975 g         4520 t
ORIGIN      
        1 ataatgatat tttaatgagc attctgaaaa aatatcgatg catttcgagc gaagatggtg
       61 gggatccctg aatgcgtttt tctcaccgac tgttcttact gctgattctc ctgctgacgg
      121 gagcaccaat actcgctcag gagccttctg acgttgcgaa aaacgtgcgt atgatggttt
      181 ccggtattgt cagttacacc cgctggcctg cgctatcagg gccaccaaag ttatgtatct
      241 tttcatcttc acgttttagt accgccctac aagagaatgc cgcaacatct ttgccttatc
      301 tgcctgtcat cattcacact caacaagagg cgatgatttc aggctgcaat ggtttttatt
      361 ttggcaacga gtcaccgaca ttccagatgg aattaaccga acaatatccg tcaaaggcgt
      421 tattattaat tgccgaacaa aataccgagt gcattattgg tagcgccttt tgtctgatca
      481 tccacaataa tgacgtcaga tttgccgtaa acctggatgc cttatcgcgt agcggtgtaa
      541 aggtcaaccc ggatgtctta atgctcgcac ggaagaaaaa tgatggataa cgataattct
      601 cttaataagc gccccacgtt taaaagagca ttacgcaaca tcagtatgac cagcatattt
      661 atcactatga tgctgatctg gttgctgctt tccgtgacct cggtgctgcc cctgaaacag
      721 tacgcgcaaa aaaacctggc actgacagca gcaacaatga cttacagtct ggaagcagct
      781 gtcgtttttg ccgatggccc tgcagcaact gaaacactgg cagcgctggg ccagcaaggg
      841 caattttcaa ctgcagaagt acgtgataag cagcaaaata ttctggcatc ctggcattac
      901 acccgtaagg atccaggcga tactttcagc aatttcataa gccactggct cttccctgcc
      961 cccatcattc agccgattcg tcacaatggt gaaaccattg gcgaagtacg cttaaccgct
     1021 cgcgacagtt caatcagcca ttttatctgg ttttcgctcg ccgtactgac cggttgtatt
     1081 ctgctggcat caggaatcgc aattaccctc acccgccatt tgcacaatgg cctggtggaa
     1141 gcactgaaaa atatcaccga tgtcgtacat gatgtgcgtt ccaaccgcaa tttttcccga
     1201 cgagtttcgg aagaacgtat cgctgagttt caccgcttcg ctctcgactt caacagtctg
     1261 ctggatgaaa tggaagagtg gcagcttcgt ttacaggcca aaaatgcgca gcttctacgt
     1321 accgcgctac atgacccatt aaccgggctg gctaaccgcg cagcgtttcg tagcggcatc
     1381 aatacgttga tgaacaattc cgctgcccga aaaacgtcgg cgttactatt tcttgatggc
     1441 gataatttca aatatatcaa tgatacctgg ggtcatgcga cgggcgatag agtcttgatt
     1501 gaaatcgcaa aacggttagc tgaatttggc gggctgcgac ataaagcata ccgcctgggc
     1561 ggcgatgaat tcgctatggt gctctatggt gtacagtcgg aatctgaagt gcagcagata
     1621 tgctcagcac tgacacaaat ctttaatctc ccgtttgatc ttcataatgg ccatcagacc
     1681 accatgacat taagcattgg ttacgcaatg accattgcgc acgcttctgc ggaaaaatta
     1741 caagagctgg ccgatcacaa tatgtatcag gccaaacacc agcgtgccga aaagctggtg
     1801 agataacaag gatatatcga tatgataaag cacctggtag cacccctgat tttcacctca
     1861 ctaatactga ctggctgcca gtcccctcag ggaaagttta ctcctgagca agtcgccgct
     1921 atgcaatctt atggatttac tgaatccgcc ggcgactggt cgctgggctt atcagatgcc
     1981 attctgttcg caaaaaatga ctacaaattg ctcccggaaa gccagcaaca gatccaaacc
     2041 atggcagcca aattggcctc gacagggcta acacatgccc gtatggatgg acacaccgat
     2101 aactatggtg aagacagtta caacgaaggc ttatcattga aacgggcgaa tgtcgtggcc
     2161 gatgcatggg ctatgggtgg acaaattcca cgcagcaatc tcaccacaca gggtttagga
     2221 aaaaaatatc ccatagccag taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc
     2281 gcagtggtga ttactccccc ttaaaacacg agcaaacaga taaaaaaagg gccagccaat
     2341 tggccagccc ttcttaacag gatgtcgctt aagcgaaatc ttagttaaga cgctctttga
     2401 tacgagcagc cttaccagta cgctcacgca ggtagtacag tttagcttta cgaacagcac
     2461 cacgacgttt gacagaaatg ctgtcaacta ccggagagtg agtctggaag acacgctcaa
     2521 cgccttcgcc gttggaaatt ttacgaacag tgaatgcaga gtgcagaccg cggttacgaa
     2581 tagcgataac cacgccctcg aatgcctgca gacgtttttt ggaaccttca acaacccata
     2641 ctttcacttc cacggtatca cccggacgga aggaaggtac gtcctgcttc atctgctctt
     2701 gttcaagttg cttaataatg ttgctcataa tttaatctct tatcctgggt aaactgatat
     2761 ctcgggggct tacgccatcc catcatgttt atgttgctgt tgtgcgtgtt ccgttttgaa
     2821 ctccgccagc aaccttgctt gctcttcagt cagagccagg ttttccagaa gttcaggtct
     2881 tctaagccag gtacggccca gcgactgttt caaacgccag cgacgtatct cggcatggtt
     2941 gcccgacagt aacactggcg gaacttccat cccttctaac acctcaggcc gcgtatagtg
     3001 cgggcaatcc agcaatcctt cagcaaagga atcttccgtt gccgaggctt catgtcccag
     3061 tactcccgga ataaaccggg aaacggagtc aatcagcgtc attgctggta actcaccacc
     3121 actgagaacg taatcgccga ttgaccattc ttcgtcaatt tcggtttgga tcacgcgctc
     3181 atctatacct tcgtagcgac cgcacaccag aatcaatttt tgattcgttg ccagttcgct
     3241 gacgcccgct tgatcaagct tgcgtccctg tggtgaaaga taaatcacct ttgcgccttc
     3301 acccgccgcg gcttttgctg catgaatggc gtcccgcaag ggttgcacca tcattaacat
     3361 ccccggtccg ccgccgtaag gacgatcgtc cacggtacgg tgccggtcat gcgtgaaatc
     3421 gcgaggactc cagctctgga tgctcagcag gccattttta actgcccggc cagttacccc
     3481 gtaatcggta attgcgcgga acatttcagg aaacaggcta attatgccaa tccacatagc
     3541 gccgtctttt accgtttatc cggtggttta aaaaccagga tcccaatcta cttcgattga
     3601 acgagtagtg agatcgactt tcttgataac ctgcccatcg aggaacggta cgagacgttc
     3661 cttgatacca aacgcatctt tcaggtttgc cttaatgacg agaacgtcat tggatccggt
     3721 ttccatcata tcgacgactt taccgagatc gtagccttca gtggttacta cctggcagcc
     3781 catcaggtct ttccagtagt agtcaccctc ttcaagctga ggcagctgcg atgaatccac
     3841 gacaatttca caattcgtca gcaggttcgc cgcatcacga tcgtcaacgc ctttcagctt
     3901 gatgatcatg tcctgattgt ggtgcttcca gctttccagc tggacttgct gccactgacc
     3961 cgccttctgg ataaaccagg gctgatagtc aaaaatgctt tcggcgtctt cggtggaaga
     4021 aaacactctg agccacccac gaataccgta agacgaaccc atttttccca aaacgatggg
     4081 atcaacaggt gcttgcgcgg tgagttgttt gctcatcatg accaccgtga cagattaagc
     4141 tgctttgttt acttctttga tcagcgcagc aacgcgatca gaaatagttg cgccctggcc
     4201 aacccagtga gcgatgcgat ccagatccag gcgagtgcct tcttcttttt cgctagcgat
     4261 tgggttgaag aaaccaacgc gctcgatgaa gcgaccgttg cgtgcattac ggctgtcagc
     4321 gacaacaacc tggtagaacg gacgcttttt agcgccgtga cgtgctaaac gaatagttac
     4381 cataacatcc tcttgtgtga ataaaacaac cggaccccat cgaggaacgg agtccggtgt
     4441 catattaaaa gcccgaaaat tttactgatt tctggagaaa atgcaatcag cagttaataa
     4501 ctctgtcgca taaggtcatt tagcgaccag gaaatcccgg cggcatcatc cccttcatgc
     4561 ttctcatcat cttcgccatt ccgcccttct tcattttctt catcatgcgc tgcatgtcgt
     4621 cgaactgttt cagaagacgg ttaacgtcct gcacctgcat accgcaaccg gcagcaatac
     4681 ggcgtttacg cgaacctttg atgatttctg gcttagcgcg ctctttcatc gtcatcgagt
     4741 tgatgatggc ttccatacgc accagcactt tatcgtccat ctgtgacttg acgttatccg
     4801 ggatctgccc catgcccggc agtttgccca taaggctggc catgccgccc atatttttca
     4861 tctggcgcag ttgctcaagg aagtcgttga gatcgaagcc gtcacctttt ttcagcttgc
     4921 tggctaattt ctctgcctgc gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca
     4981 gtacgtcgcc catgccgaga atacgcgacg cgatgcggtc cggatggaac ggctccagcg
     5041 cctcagtttt ctcgccaaca ccgaggaact tgatcggttt gccagtgatg tgacgaatag
     5101 agagcgccgc accgccgcgg gcatcgccgt ccactttggt caacactacg ccggtaagcg
     5161 gtaacgcttc attgaatgct tttgccgtat tggccgcatc ctgaccggtc atggcgtcaa
     5221 ccacaaacag ggtttcaacc gggttaatcg acgcatggac ttgtttgatc tcgtccatca
     5281 tcgcttcgtc aacgtgcaga cgaccagcgg tatccaccag cagcacgtcg tagaatttca
     5341 gtttggcttc tttcagcgcc gcgttaacaa tatcaaccgg cttctgacca acatcagaag
     5401 ggaagaaatc aacgcccacc tgctctgcca gcgtctcaag ctgtttgatt gccgccgggc
     5461 gatagacgtc agcagaaacc accagcactt tcttcttgtg cttctcgcgc aggaacttac
     5521 cgagtttacc aacgctggtt gttttaccgg caccttgcag gcctgccatc agcacgaccg
     5581 caggcggttg cgcagccaga ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt
     5641 cgttacggac gattttgacg aactcctgcc ccggcgtcag gctcttatta acttcatgac
     5701 caaccgcttt ctcttttacg cgattgataa actcacgcac taccggcaga gcgacgtccg
     5761 cctccagcag cgccatgcgc acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc
     5821 gtccacggcc actgatattg cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca
     5881 ttgtctctcg cctggggtgg aaacggttgg ccgcaatcgc gacacatcat cagtattttg
     5941 ccgcagtata acatgaaggc gtctttgttg ttatgcaacg gttggagcag cgttcacctg
     6001 acgctatact gcttctcttt cttattgctc aaactgtcga catcactatg cccgtttttg
     6061 ctctgctcgc gcttgtcgcc tactccgtca gtcttgcgct gattgttccc ggtctgctgc
     6121 aaaaaaacgg cggctggcgg cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc
     6181 acgcaatcgc tctggaagcc cgcatcctgc ccgacggtga tagcggacaa aacctcagcc
     6241 tgctgaacgt tggttcattg gtcagtttga tgatctgtac ggtaatgacc attgtggctt
     6301 ctcgcaatcg tggctggttg ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg
     6361 cgctggcaac cttcatgccc aatgaataca tcacccatct ggaagctacg cctgggatgc
     6421 tggtgcacat tggcttatcg ctcttttcct atgccacgct aattatcgcc gccctgtacg
     6481 cgctgcaact ggcgtggatt gattaccaac tgaagaacaa gaagctggcg tttaaccagg
     6541 aaatgccgcc attgatgagt atcgagcgta aaatgttcca catcacgcag attggcgtgg
     6601 tgctgctaac gctcacgctt tgcactggcc tgttctacat gcacaactta tttagcatgg
     6661 aaaatatcga caaggctgtg ctctctatcg tggcgtggtt tgtctatatt gtgctgctat
     6721 ggggacatta tcatgaagga tggcgtggac gtcgcgtcgt ctggtttaac gttgcgggtg
     6781 cagtcattct gacactggcc tacttcggca gccgaattgt ccagcagtta atcagctaaa
     6841 cccagaaaag gagtttcccc ctggaacaca tttctactac tacgttgatc attattctga
     6901 tcatcatggt ggtcatttca gcctattttt ccgggtccga aaccggaatg atgaccctca
     6961 accgctatcg tctgcgacat atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa
     7021 aattgctgcg taagccagac cgcctgataa gcctggtgtt aatcggcaat aacctggtca
     7081 atattcttgc ctccgcgctc ggcactattg ttgggatgcg tttgtacggc gatgcgggcg
     7141 tggcaattgc gactggtgtg ctgacttttg tcgtactggt ttttgctgag gtattgccga
     7201 aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc gagtagtttt ctgctggctc
     7261 ccctgcaaat tttgatgatg ccgctggtct ggttgctgaa tgctatcacc cgtatgctga
     7321 tgcgcatgat gggtatcaaa accgatatcg tggttagcgg ctctttgagc aaagaagagt
     7381 tgcgcactat cgtgcacgaa tcgcgctcac aaatttcccg tcgcaatcag gatatgctgc
     7441 tgtcggtgcc cgatctggaa aaaatgaccg ttgatgacat catggtgccg cgcagtgaaa
     7501 ttatcggtat tgatatcaac gatgactgga aatctattct gcgccaactc tcccactcac
     7561 ctcacgggcg catcgtgctc taccgtgatt cgctggacga cgccatcagt atgctgcgag
     7621 tacgtgaagc ttggcggttg atgtcggaga aaaaagagtt caccaaagaa accatgctgc
     7681 gcgccgcgga cgagatctat tttgtgccgg aaggtacgcc gctcagcacg cagttggtga
     7741 agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg
     7801 ggctggtgac ggttgaagat attctggaag agattgtcgg cgatttcact acgtcgatgt
     7861 cgccaacact tgccgaagaa gtcacgccgc aaaacgacgg ttcggtgatt attgatggca
     7921 ctgccaacgt gcgggaaatc aacaaagcct ttaactggca tctaccggaa gatgatgccc
     7981 gcacggttaa tggcgtcatt cttgaggcac tggaagagat ccctgtcgta ggcacccgcg
     8041 tgcgtattgg cgagtacgat atcgatattc tcgacgtaca ggacaatatg attaagcagg
     8101 taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc ggagtaacga aaacggcccg
     8161 gcattcgcat gcagggccgt gaattattac gaaaacagaa attacgcttt tgctttcgct
     8221 acagttacca tcgccgcacg aatcgtacga ccattcagcg tataaccctt ctgcataatg
     8281 cccagtacgt tacctggcgc aacgtcatca gattccacca ttgcgatagc ctgatgcaca
     8341 ttcgggtcca gcggcacgtt agtttcggcg atcacttcaa caccaaactt acgcacaaca
     8401 tccagcatcg acttcagcgt cagctcaatg ccttcaacca tcgcagacat atccgggtta
     8461 gctttatcag ccacttccag cgcacgatcc aggctatcaa tcaccggcag caattcgttg
     8521 atgaatttct ccagcgcgaa tttgtgggct ttttcaatat ccagttcagt acgacgacgc
     8581 aggttttcca tttcggcttt tacacgcaaa atgccgtcac gttcacgggt ctgggcttca
     8641 gccagctgag cttcgagatt cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa
     8701 gcttctggct caactgcctc aatctcttcg tgctgatcca tgataatttc ttccggggct
     8761 tgcccctcag gcgttttctg ttctttacta ctcatgaatt tctccgcgtt tttttcgcat
     8821 tcatctcgct aacttcgctt attatgggga tcagtttcag ggtttcaagg gaagcactca
     8881 cattgtcatc aatcttcgca acaaggacct cggaaaaatg aataatcatt tcaagtgtat
     8941 tggcattgtg ggacacccac ggcaccctac tgcactgaca acacatgaaa tgctctaccg
     9001 ctggctgtgc acaaaaggtt acgaggtcat cgttgagcaa caaatcgctc acgaactgca
     9061 actgaagaat gtgaaaactg gcacgctcgc ggagattggg caactggctg atctcgcggt
     9121 agtcgttggt ggcgacggta atatgctggg cgcgacgcgc acgctcgccc gttacgatat
     9181 taaagttatt ggaatcaacc gtggcaacct gggtttcctg actgaccttg accccgataa
     9241 cgcccagcaa caattagccg atgtgctgga aggtcactac atcagcgaga aacgtttttt
     9301 gctggaagcg caagtctgtc agcaagattg ccaaaagcgc atcagcaccg cgataaatga
     9361 agtggtactt catccaggca aagtggcgca tatgatagag ttcgaagtgt atatcgacga
     9421 gatctttgcg ttttctcagc gatctgatgg gctaattatt tcgacgccaa caggctccac
     9481 cgcctattcc ctctctgcgg gcggtcctat tctgaccccc tctctggatg cgattaccct
     9541 ggtgcccatg ttcccgcata cgttgtcagc acgaccactg gtcataaaca gcagcagcac
     9601 gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa atcagttgcg acagccagat
     9661 agcactaccg attcaggaag gtgaagatgt cctgattcgt cgctgtgatt accatctgaa
     9721 tctgattcat ccgaaagatt acagttattt caacacatta agcaccaagc tcggctggtc
     9781 aaaaaaatta ttctaatttt acgccagcct ctttactgta tataaaacca gtttatactg
     9841 tacacaataa cagtaatggt ttttcataca ggaaaacgac tatgttggca caactgacca
     9901 tcagcaactt tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa
     9961 taactggcga aaccggtgcg ggtaaatcta ttgcaataga tgccctcggt ctttgtctcg
    10021 gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc tcgcgctgac ctgtgcgccc
    10081 gtttttctct gaaagatacg ccagcggccc tgcgctggct ggaagaaaac cagcttgaag
    10141 acgggcatga atgtttgctt cgtcgcgtga tcagcagcga tggtcgctcc cgtggtttca
    10201 tcaacggtac agctgttcct ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga
    10261 tccatggtca gcacgctcat cagttactca ccaaacccga gcaccaaaaa ttcctgcttg
    10321 atggctatgc caatgaaacc tctctactcc aggaaatgac cgcacgttat cagttgtggc
    10381 atcaaagctg ccgtgacctc gcgcatcatc aacagttaag tcaggaacgc gctgcccgtg
    10441 cggaactgct gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cccggagagt
    10501 ttgagcaaat cgacgaagag tacaaacgtc tggcgaacag cggtcaattg ctgaccacca
    10561 gccagaatgc attggcatta atggccgacg gtgaagacgc aaacctgcaa agtcagcttt
    10621 acacggctaa acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac
    10681 ttgatatgct ggaagaagct accatccaga ttgttgaagc cagcgacgaa ctgcgccact
    10741 actgcgatcg tctggatctc gatcccaacc gactgtttga acttgaacag cgcatctcaa
    10801 aacagatttc gctggcacgt aaacatcacg tcagccctga gacattgcca cagtattacc
    10861 agtcgctact ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg
    10921 cactggcggt aacgaaacat catcagcagg cactggaaac ggcgcgcgca ttacaccagc
    10981 aacgccagca ttatgcagaa gaacttgcac agctgatcac cgacagtatg catgcgctct
    11041 caatgccgca tgggcagttt acgattgatg tcaaatttga cgagcatcac ctgggcgctg
    11101 acggcgccga tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta
    11161 ttgccaaagt cgcatccggt ggtgaattgt cccgcatcgc attggcaatt caggtcatca
    11221 cggcgcgtaa aatggaaacc ccggcactga tttttgatga agtggatgta gggattagcg
    11281 gtccaacagc ggcagttgtc ggcaaactgc tgcgtcaact cggcgaatca actcaggtga
    11341 tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca tcaacactat tttgtcagca
    11401 aagaaaccga tggtgcgatg acagaaacgc atatgcaatc cctgaataaa aaaacgcggt
    11461 tacaagagct ggcgcgcctg cttggtggca gtgaagtcac acgtaacaca ctggcgaatg
    11521 cgaaagaact gcttgcggcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa
    11581 acgccgtaag accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca
    11641 tattacagat gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt
    11701 aaaacgctga ctgctgcagc agcagtacta ttgatgttga ccgcaggctg ttccactctg
    11761 gagcgagtgg tttaccgtcc tgacatcaac caggggaact atctgaccgc taacgacgta
    11821 tccaaaatac gtgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg
    11881 atgtccgatc catttggtac gaatacctgg ttctatgtct tccgccagca accaggtcat
    11941 gaaggtgtaa cgcagcaaac gctgacgcta acctttaaca gtagcggtgt gttgaccaat
    12001 attgataaca aacctgcgct gagtggtaac taataataaa gttgctctca aagacgttaa
    12061 aaaaggtgct caatgagcac cttttttctg tccgttattt attcgctgat ttttctgctc
    12121 gttgcctgcg aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc
    12181 catcatgcac actatcgctt aattttgccg gacggctgta aatgccgact ttgttttcag
    12241 ttaaatcgat atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg
    12301 tcgcgccctc ctgcagcgtc acgcgctgca ggtactgctt cttaggtagc gcataagcca
    12361 cctcaacggc aattttacct ggcactgtaa acctctttcg cacgaaccgt aaaagcctgg
    12421 accatattag acgccagctc tttaaacacg cgaccaaaag cgagttcaat caacttattg
    12481 gtaaactcaa agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac
    12541 ttccatccac caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg
    12601 ttactggtca actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct
    12661 accgccgcag tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc
    12721 aaaaactgag gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag
    12781 ggtaccagtg cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca
    12841 aataatatca tttatcctgc agtaaaaaaa acgctatccc ggcgctgggt aacatccggt
    12901 tcatgctaag atagagcctt gtcccccgca ggattgatat ggggtatttt cgatttcaga
    12961 ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac
    13021 catcgcgctt aacaagcgcg cccgtcacga atactttatt gaagaagagt tcgaagcggg
    13081 acttgccctg caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atattagcga
    13141 cagctatgtc cttctgcgcg acggagaagc gttcctgttt ggcgcaaaca tcacgccaat
    13201 ggctgtggct tctacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa
    13261 ccagcgcgaa cttgattcgt tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc
    13321 gctctccctg tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa
    13381 gaaacagcac gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg
    13441 tatcatgaaa aacgcccacc gttaaacctg cactccaatt cttgacctgt tcctcaccgc
    13501 gcctccctct ccggcggcgc gaatgaacat cttattggct atcacatccg acaaaaatgt
    13561 tgccatccca ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga
    13621 taacgccatt gaggctggtc atagcgctca taaatctggt atacttgccg ttacacattg
    13681 gggctgattc tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg
    13741 gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta
    13801 ataacctgct tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg
    13861 tcaaacccaa aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc
    13921 aggctagttt gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta
    13981 agcatgtagt accgaggacg taggaatttc ggacgcgggt tcaactcccg ccagctccac
    14041 cactttaaga aggactacaa ccggacagta gcaataaata cagccactta cggacacaag
    14101 ccagacagta gtcggactga aaaaagacaa aaatatacac gtgaaatgca cgtgcacttc
    14161 taaaaaaccc cagttatcac cgactggggt ttttcaattt gtaactaagg gtaacaaccc
    14221 ccccgttttt ttcatctcac tcaccttgat actgtttatt tgtacagtta aaaataatac
    14281 tgtatacaaa cacagtatag agggactttt atgcgtattg agatctgcat agccaaagaa
    14341 aaaatgacta aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc
    14401 atcagtaaac gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt
    14461 tctgttacgc gcaccgccga taaagattca gctaaaactt ttgttcagga gactctgaaa
    14521 gatacctgga agtctgctga cgagtggttt gttcactaat taacacgtaa aatcggtaac
    14581 ggctggaaat cattcaatac tcgcactatc gaaagttcac cagccaaccg cagcacgtcc
    14641 tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc
    14701 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tactttttct
    14761 aggcttccag caatgaggtt tcttcctccg ttgcaatttc cagatctgca gcatcctgaa
    14821 gtggggcgca ccacgcaaac atcacaaaat attgtcgctg cgatgaacct gtacaataaa
    14881 taatgaatca cggtacgttt ctgtgtataa cgtactgtag ttttctttac gatttatagc
    14941 tgtactggtg agccatgagt aatctgaatc catgcatggc gtgtggtgcc gttgtgcatt
    15001 ttctgcgtct cttttactgg tccgaagccg atgatgctga agcagtcctc tcctctagcc
    15061 tcatccctgt atagcacgca tcaaataatc tgggatatca tttcgtagtt ttacaatatc
    15121 aatatccgaa tcgatatgtt tctggtaaaa cttgtccgca agcaagtttg ctatggtatc
    15181 tgatgtacga ttttttacat cccggctcat accatcacaa agagcatcca ttttcttgcc
    15241 aaaagaagca ctccccatta ttacatgatt aatagaactt tgtgctgcct taacatcaag
    15301 ggttagttga ttctcctggc tcacaagaaa aaagactctt tgcctctctt cagtggaaat
    15361 ttttgcattt ttttgaaaac agtaattatc aatctcctgg ttgatcaccc ttgagggata
    15421 tactatccgt tcatctttta tccactcatc aatatcttta gtactctgcc ttctcaccgt
    15481 ttccagtata ttgccgctaa atttgcgaac agaaaagcta atagtccgtt ctccaattga
    15541 acattttacg acctcatcat tcttccctcg gaaagagttc ctgaaggagt tctctctatg
    15601 gcaacttata ccaaaagagc tgaaagacat acctgtagta ttcatcggca ttataattcc
    15661 tttttataaa aaagtgttta caatattgca tacttgcgtt tattgtagcc agaaaaaaca
    15721 tgcatgcaat aatattctat taaaaattgt attgttttat tcacatatca tgagcggcat
    15781 gttgattctt gttaccaata tagttcacaa tatggattaa aaaggataat aatatgccag
    15841 tcatattaaa tttttctaat ggaagtgtat tgccggaaaa tgagctggag gctttacgac
    15901 atatagcaag aagcaaccag aatgacacta ttactatagg aggccgtaat atgaggcttc
    15961 attatatcca gtttatggat ggttttagtg ttgagcctat tcttggtgga ctttgggacc
    16021 atcttggggc aagagaggct catcatctcg cagaccgcct gacaagacaa cttaacggag
    16081 gtaacacttt tctgcaggca tacagcttat atctggagca gaggcaagct gccccgcttg
    16141 tgcaggagag cgtcataaaa acactactag atcgaataaa ttcgaatgca tttcccgtta
    16201 gtttacaaga cttttcctgt actgaggaac atcttaattg tccgataacg ctacatattc
    16261 ctgagacagg tgtttttgtc agaaatgctc gaaattcaga aatatgtgca ttatatgatc
    16321 aggaagcatt gactgaactt atcctgcgta acgctctcca ccccctcagc cgtgacccct
    16381 ttgctccaga aatgattata agcaaagaca agtgtcattt taatataaca aaacaatgtt
    16441 tttacgcatt acccatatac ccacttcaac aaaacagtat ttaaaaataa aagcacaata
    16501 taagaagcat attttcaata agttaatcac catataacaa acatatagcg aggagactat
    16561 ttatgcccaa aatatcatca gttgtatcat catgttacca tctgttcagt gaacatcaac
    16621 aactttcaaa tgaaacaaca atgacgaacc ccgtctccag aagaattgtt cataaagaat
    16681 atggtatatc tttaaaatcc gttcctgtat ggttggctac agctaaaact ccccttgctc
    16741 tactcaatgg cagacataca agaagtcact catttattat tgcagggacc ccaggaatgg
    16801 gaagcaggag cggagcccaa tactatgcca taaacagtga tgataaacgc tcccgaatag
    16861 acattgactc tttattttta aaaaagttaa ataatgtgcg aaatcaaaat aaatttccaa
    16921 tcgatgtaaa agaaacggtt ataaaactac aagggcaaaa attcacatgc attgaagatt
    16981 tttataaaag gtataatgaa accaggttaa aggccaatac caatatccaa caagaacaaa
    17041 ttgcagatga agtaaaatca cttacatatt tgattccttc agaaaaaaaa gagatgtgga
    17101 tatataaaaa caatggaaaa gataatgcaa aaccaaactt aggagagcga gacgtaagaa
    17161 tgttcgaaaa tattagttct gatgatacag ataagataac aggaaggaaa ttttcagagt
    17221 taggtgagta tctttattca gggaatgtaa taaaactcag tcagttatca attcgttact
    17281 tacccaatat cagctcaatc tcattaatag agacaaaaca gagtttgttg ctacatcgat
    17341 tgtattcaga tgaagtactt cagagaaatg gaacgcttat cccgacacca ctacatgaag
    17401 aaaaatcaat tccagctgac aatataaaaa caatgctcaa caacataccg acttacaaaa
    17461 tgttaccgcc attcacagaa acacaaggta attgttcttc tggcgcagcc acgtttttac
    17521 gcaaatcagg cgccgaagaa aaagatattc ttgcatgtag cccccgaaat tatgggctgc
    17581 atcataacat aaaaacatgg gaccccttgg ttagaaatta aggatccaaa tattatatat
    17641 ttcatataaa gcaagaaaaa aatattgtct gaataattgt t
//
LOCUS       DAZFKY010000097        17171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFKY010000097 DAZFKY010000000
VERSION     DAZFKY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(377..1573)
                     /gene="hemY"
                     /locus_tag="ACHKCL_002859"
     CDS             complement(377..1573)
                     /gene="hemY"
                     /locus_tag="ACHKCL_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5763794.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1576..2769)
                     /gene="hemX"
                     /locus_tag="ACHKCL_002860"
     CDS             complement(1576..2769)
                     /gene="hemX"
                     /locus_tag="ACHKCL_002860"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5763795.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADTPAA
                     APQGE"
     gene            complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHKCL_002861"
     CDS             complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHKCL_002861"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5763796.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHKCL_002862"
     CDS             complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHKCL_002862"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5763797.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHKCL_002863"
     CDS             4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHKCL_002863"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5763798.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHKCL_002864"
     CDS             complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHKCL_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5763799.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(7924..8066)
                     /locus_tag="ACHKCL_002865"
                     /pseudo
     CDS             complement(7924..8066)
                     /locus_tag="ACHKCL_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8225..8428
                     /gene="yifL"
                     /locus_tag="ACHKCL_002866"
     CDS             8225..8428
                     /gene="yifL"
                     /locus_tag="ACHKCL_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5763800.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8465..9289
                     /gene="dapF"
                     /locus_tag="ACHKCL_002867"
     CDS             8465..9289
                     /gene="dapF"
                     /locus_tag="ACHKCL_002867"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5763801.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9286..9993
                     /gene="yigA"
                     /locus_tag="ACHKCL_002868"
     CDS             9286..9993
                     /gene="yigA"
                     /locus_tag="ACHKCL_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO5763802.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            9990..10886
                     /gene="xerC"
                     /locus_tag="ACHKCL_002869"
     CDS             9990..10886
                     /gene="xerC"
                     /locus_tag="ACHKCL_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5763803.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10886..11602
                     /gene="yigB"
                     /locus_tag="ACHKCL_002870"
     CDS             10886..11602
                     /gene="yigB"
                     /locus_tag="ACHKCL_002870"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5763804.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHKCL_002871"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHKCL_002871"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5763805.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13893..14795)
                     /locus_tag="ACHKCL_002872"
     CDS             complement(13893..14795)
                     /locus_tag="ACHKCL_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFO5763806.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHKCL_002873"
     CDS             complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHKCL_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5763807.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHKCL_002874"
     CDS             15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHKCL_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5763808.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16012..16962
                     /gene="corA"
                     /locus_tag="ACHKCL_002875"
     CDS             16012..16962
                     /gene="corA"
                     /locus_tag="ACHKCL_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5763809.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16987..>17171)
                     /locus_tag="ACHKCL_002876"
     CDS             complement(16987..>17171)
                     /locus_tag="ACHKCL_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763810.1"
                     /translation="CKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTT
                     IMLSAIPSAKMSLSAH"
BASE COUNT         3921 a         4504 c         4581 g         4165 t
ORIGIN      
        1 caagctcagg ttaaatattc actcaggaag ttattactca ggaagcaaag aggattacag
       61 aattatctca taacaagtgt taagggatgt tatttcccga ttctctgtgg cataataaac
      121 gagtagatgc tcattccatc tcttatgttc gccttagtgc ctcataaact ccggaatgac
      181 gcagagccgt ttacggtgct tatcgtccac tgacagatgt cgcttatgcc tcatcagaca
      241 ccatggacac aacgttgagt gaagcaccca cttgttgtca tacagacctg ttttaacgcc
      301 tgctccgtaa taagagcagg cgttttttta tgtgtcagga aggtcccgga ggtgcttgcc
      361 tccgggtgag aaggaactac tgtggtgggt tgttctgcaa cgttaacatc aaaccgtcgc
      421 gacgcatcgc tgcagcttct tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg
      481 cgtaatcgta ggcgtccgga cgttgtttca gcgctgcgcg gaaggcgagc gatgcttcct
      541 gccattctcc gtgcttcatc agtgactggc ccagtgtgct ccacaacagc gggcgatcgc
      601 cgacgttttt gatttgctgg cgcagcactt tttccagctg ttccggattg tttgttttca
      661 gtcgaggaat cggcagcagt aggcgatcgt cgtactggcg tttcaggcca tcgatgataa
      721 tttgctgggc agtatcatga tcgtcacatt caataagatg ttccgccatt gccacctgca
      781 acgctacctg atgacgcgtt ttccggcttt ggtttttcca ccagttacgc aaaccttcgc
      841 taccgttatc ggcacgcgcc tgatccatca ggccaatcca tgcctgttgt tccagcattg
      901 cacgatgttc ttcatcacca acatgggctt tcgccattga tgggataata tccagcagcg
      961 aactccatgc acctgtgcgg atatacgcct gttccgccag acgtaatact tccggatggc
     1021 gtggcgtaac ttccagcagc ttatccacgc cgtggcgtgc agcatggttt tcattacggg
     1081 ccagttgcag acgtacacgg gtgatttcta ccggaatggt gtcattgccg gccagctccg
     1141 ctgcgcgttc cagatgttgg ttggcgcgcg cttcatcacc acgttgttgc gcggcttcgg
     1201 cagccagtag atagttcacc accggttgtt cagcgtgatc ggcatttttc gccatcagct
     1261 tttcaacttg ctgataatcg ccttccgcca gtttcagcag cgcctgttcg gtctgcttac
     1321 gtgcacggcg acgcttacgc ccgacaaacc acccacgggt gtgcgcgcca gtgcggaaga
     1381 tccgccgcag tagccactca atggcaaaca gcactaccat cgccagaatc aatatgatcg
     1441 ccaggcccgt gacgctggtt tcgatattgt agttgtcggt ctggatcagc acataaccct
     1501 gatgaccggc aatcatcggg ccaaccacga tccccgcaat cagcaacaca aagagcaata
     1561 acacttttag catggttatt ctccttgcgg cgcggctgcc ggagtatcag cttgcggtgc
     1621 aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt gtgccagcag
     1681 gttacgcacg cgagtctgca tcagtttttc cagcatcgcc tggctttgca gggtttccgg
     1741 aagatccatc gagatatttt gctggcttaa ctggtctacc tcgtcgagga acgctttggt
     1801 ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg
     1861 gcgataagtc tcttgctggt gacgcggtac agcttgtgct gcgaccagca ggcgagagcg
     1921 aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc
     1981 acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt gcagattgat
     2041 acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc
     2101 gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat taagcttaag
     2161 gatgatgccg tcataatcca cctgcgatac tgcagaaagg ctggcgatat catcggtaat
     2221 tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact
     2281 tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag
     2341 tttcaccaga aaatcggcct gagccagcag ccaggtttta gcatcgctgc cggaaatggt
     2401 ggcgaccttt tgttggactt catccaactg ttttgccagc gtttcttgct gacgattcgc
     2461 ctgcttaagc tgtgcagctt gttgcttaat aatgccttcc agctcgactt tttggctctc
     2521 ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg cggtctgatt
     2581 gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat
     2641 agccaccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg
     2701 ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg ttttttcttg
     2761 ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt
     2821 gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa
     2881 acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat accattgtgg
     2941 gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc
     3001 gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata
     3061 acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg
     3121 accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa
     3181 ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct gtccgcttac
     3241 ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta gtcggggcca
     3301 tttacgatct tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc
     3361 aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg
     3421 acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg
     3481 gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca tgccggggcg
     3541 tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc
     3601 agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc
     3661 tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc aataagctcg
     3721 gcgtagctac caattggcac ctgacatcca ccttcgagac gggtattcat ggcgcgttct
     3781 gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgt
     3841 gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg aagagaaatc
     3901 tcgggtggca acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg
     3961 gcaagaatga tggcatcgta ttcgccgtta tccagtttgc tcaggcgagt gccgacgttg
     4021 ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt
     4081 aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc atagttattg
     4141 gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccaggcc cagaccttgc
     4201 gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg attttcgagg
     4261 agcgcgactt ccagctcttt tacaaataag ccttttccgc cgactttcgc cagcggcgta
     4321 tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg
     4381 ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt
     4441 gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca
     4501 tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa
     4561 ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg taataaggaa
     4621 tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca
     4681 gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca
     4741 ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta
     4801 ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta
     4861 cctctatatt gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg
     4921 cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt
     4981 gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg
     5041 cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg
     5101 aatgtcagta caggacccgc cgaaaggtga gcttccaatt actggtgtat acaccatggg
     5161 cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca
     5221 atcctggctc gatagcgaag agcgccagtt gctacaacgt aaatgcagcc tgctggaaag
     5281 ctgggccgcc tcgctgggtg tggaagttag cttcttcctg attgatgaaa accgttttcg
     5341 ccacaacgag agtggcagtc tggggggcga agattgtggc tccacccagc atatactgct
     5401 gcttgatgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat
     5461 ggtgccgtgc gacgaagaag agcattacga tgactacgta atgacgctct acgcacaagg
     5521 cgtgctgacg ccaaatgaat ggctggatct cggcggctta agctcgcttt ccgctgaaga
     5581 atacttcggt gccagccttt ggcaactcta caagagtatc gattctccat acaaagcggt
     5641 gctgaagaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac gcctgctggc
     5701 gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgatcctta
     5761 ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt tcacccgtct
     5821 ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc gtgaacgcgc
     5881 ctgcgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg ggtgggacga
     5941 agctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg tgcgtgaggc
     6001 gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc gctttgcgcg
     6061 tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct
     6121 gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc agatttcacc
     6181 cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccggg ctaaccgttc
     6241 aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc atcagccgct
     6301 ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg gcctgctgac
     6361 ctcgcgcacc cgcttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga
     6421 gatggtcgcc gacgtgtcgc accatttccc gttgcgctta cctgcaccga caccgaaggc
     6481 gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc
     6541 gacagctgcg ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag
     6601 ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtatc gcaactcgtg
     6661 gaatgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac
     6721 tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta
     6781 tagccagcat ctgcgcggct taattcgtac tcgcgtgcag caactggttt ctgagtgtat
     6841 tgaattgcgt ctttccagca cccgccagga aaccgggcgt ttcaaggcgc tgcgcgtttc
     6901 tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta tcggtacaga aactggagaa
     6961 cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt
     7021 tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg aagggatcat
     7081 ccagttcttt ttcgaagaaa cgcaagatga gaatggcttt aatatctaca ttctcgacga
     7141 aagcaatcgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc tggtacgtga
     7201 cgtcagtcgc ttctactcgt catcgcatga ccgttttacc tacggctcaa gcttcatcaa
     7261 cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc
     7321 gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg atacgccgct
     7381 attacagcag tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat
     7441 cttagcggaa actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc
     7501 ggaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc
     7561 cgccttgttt ggttgccagc catacctggt gcagcggttc ctggcggttg ataatgattt
     7621 tgctgccatt ctcaaaggta atggtcagca cgccgccgtt gatttcgcag tcgatatcgc
     7681 tgtcgccatc ccagtcgtcc agacgctctt caatggtcag ccacagttga tcagccaggc
     7741 gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc
     7801 ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttacggc atgattcatc
     7861 cgtcaccgga ataatgatgt ctctgtgtag cgaaagattt gtctcttcat tagggcgcag
     7921 ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc gagcgagcat
     7981 cctcacgctg acggaactaa aaaagccaac aaacaaacca cattgcgata gtgcataaag
     8041 ccatcctggc gtgaggtgtt gatcacgaaa ctgccagcag aacataaatc ctcacgagta
     8101 agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt
     8161 gcttttacgc cttctcctgc gatgatagaa agcagaaagc gatgaacttt acaggcaatc
     8221 cataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca gcctgacggg
     8281 ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaatgcac cgccgccgac
     8341 caaaccggta gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc gcgccactgg
     8401 cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta
     8461 aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt
     8521 aacgcagaat gtcttttttt caccggagct gattcgtcgc ctagctgatc ggcacctggg
     8581 ggtagggttt gaccaactgc tggtggttga gccgccgtat gatcctgaac tggattttca
     8641 ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gcgcacgctg
     8701 ctttgcccgt tttgtgcgtc tgaaagggct gaccaataag cgcgatatcc gcgtcagcac
     8761 cgccaacggg cggatggttc ttaccgtcac cgatgatgat ctggtccgcg taaatatggg
     8821 cgaacccaac ttcgaacctt ctgccgtgcc gtttcgcgct aacaaagcgg aaaagaccta
     8881 tattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccaca
     8941 ttgcgtgatt caggtcgatg atgtcgatac tgcggcggta gaaacactgg ggcctgttct
     9001 ggaaagccac gagcgttttc cggagcgcgc caatatcggt tttatgcaag tggttaagcg
     9061 cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag
     9121 cggcgcatgt gcggcggttg cagtagggat tcagcaaggt ttgctggccg aagaagtacg
     9181 cgtggaactc ccaggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata
     9241 tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg
     9301 gaagaactgc aggaaacact cacggagctt gatgaccggg tggttgtcga ttatctgatt
     9361 aaaaatcctg agttttttat ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat
     9421 ccggtacgcg gtaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt
     9481 catgttctgg aagagaacat ggcgctgttg atggaacagg ctatcgccaa cgaaggcctg
     9541 ttttatcgct tactctacct gcagcgcagt ctcaccgccg ccagcagtct tgatgatatg
     9601 ctgatgcgct ttcaccgctg ggcgcgtgat ctcggcctgg caggtgcgag tctgcgcctg
     9661 tttccggatc gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt
     9721 cagtctttcg aaccgctgcg tattcagcgt ctggggcagg agcagcatta tctggggccg
     9781 cttaacggac cggagctgct ggtggtacta ccggaagcga aagcggtggg atcggtggcg
     9841 atgtcgatgc tgggaagcga tgctgattta ggcgtggtgc tgttcaccag ccgcgatgcc
     9901 agtcactatc aacaagggca gggaacgcag ttacttcatg aaattgcgct gatgttgccg
     9961 gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct
    10021 acctacgtta tctgagcgtg gagcggcagc ttagtccgat aactctgctt aactaccagc
    10081 gacagcttga ggcgatcatc aattttgcca gcgaaaacgg cctgcaaaac tggcagcaat
    10141 gcgatgcagc gatggtgcgc aattttgctg tacgcagtcg ccgtaaaggg ctgggcgcgg
    10201 caagcctcgc attacgcctc tctgcgctac gtagcttttt tgactggctg gtcagccaga
    10261 acgaactcaa agctaacccg gcgaaaggtg tttcggcacc gaaaacgcca cgtcatctgc
    10321 cgaaaaatat cgacgtcgat gatataaatc gcctgttgga tattgatatc aacgatcccc
    10381 tcgctgtgcg cgaccgtgca atgctggaag tgatgtacgg cgcgggcctg cgtctttccg
    10441 agctggtggg gctggacatc aaacacctcg acctggagtc cggcgaagtg tgggtgatgg
    10501 ggaaaggcag caaagagcgc cgcctgccga ttggtcgtaa cgctttgtca tggattgagc
    10561 actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac
    10621 tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac
    10681 aagggctgaa taatcacgtt cacccgcata aattacgtca ctcgttcgct acgcatatgc
    10741 tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc aacctctcca
    10801 ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc
    10861 atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc
    10921 gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga
    10981 gcaggaggcg cttacctttg tgcaaaatta ccatccggcg ctgcgcagct tccagaatga
    11041 agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt
    11101 gacgcgctgg cgttttcgct caatagaaca agcgatgctt gacgccggat tgagcgcgga
    11161 agaagccagt gcaggcgcac acgcggcaat gatcaacttt gctaaatggc gcagcaggat
    11221 cgacgtcccg cagcaaaccc acgacacctt aaaacagctg gcgaagaaat ggccgctggt
    11281 ggcgatcacc aacggtaacg ctcagccgga actgtttggt ttgggggatt attttgagtt
    11341 tgtgctgcgc gctggcccgc acgggcgctc gaaaccgttc agcgatatgt acttcctggc
    11401 ggcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gtaggtgacg atctcaccac
    11461 tgacgtgggt ggggcaattc gcagcggaat gcaggcctgt tggatcagac cggaaaatgg
    11521 cgatctgatg caaacctggg atagccgttt actcccgcat ctggaaattt cccggttggc
    11581 atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag
    11641 ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac
    11701 ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccgcg cagcaacctt
    11761 ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg
    11821 ctgatgagcg tggaaaactg ctcgccatac tcgattatgg ctgtgacgtt taccaacaaa
    11881 gcggcggcgg agatgcgtca tcgtatcggg caactgatgg gcaccagcca gggcggcatg
    11941 tgggtcggca ccttccacgg gctggcgcac cgtttgctgc gtgcgcacca tatggacgcc
    12001 aatctgccgc aggatttcca gatcctcgat agcgaagacc agctacgcct gcttaagcgt
    12061 ctgatcaaag ccatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac
    12121 atcaacagcc agaaagatga aggcctgcgt ccgcatcata ttcaaagcta cggtaatccg
    12181 gtggagcaga cctggcagaa ggtgtatcag gcgtatcagg aagcgtgcga tcgtgcaggc
    12241 ctggtggact tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa caagccgcat
    12301 atcctgcaac actaccgcga acgttttacc aacatcctgg tggacgaatt ccaggatacc
    12361 aacaacattc agtacgcgtg gatccgcctg ctggcgggcg acaccggcaa agtgatgatc
    12421 gtcggtgatg acgaccagtc aatctacggc tggcgcgggg cgcaggtgga gaatattcag
    12481 cgtttcctca atgatttccc cggtgccgaa actatccgtc tggagcagaa ctatcgttct
    12541 accagtaaca ttctgagcgc cgcgaacgcc ctgattgaaa acaacaacgg gcgtctgggt
    12601 aaaaaactgt ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac
    12661 gaactcgatg aagcgcgttt tgtcgttaac cgcatcaaaa cctggcagga caacggtggg
    12721 gcgctcgccg agtgcgccat tctctaccgc agcaacgccc agtcgcgggt gctcgaagag
    12781 gcgttattgc aggccagtat gccgtaccgt atttacggcg ggatgcgctt cttcgaacgc
    12841 caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg
    12901 gcctttgagc gtgtggtgaa tacgccaacg cggggtattg gtgaccggac gctggacgtg
    12961 gtacgtcaga catcgcgcga tcgccagtta acactctggc aggcatgtcg tgaactgttg
    13021 caggaaaaag ccctcgccgg acgtgctgcc agcgccttac agcggtttat ggaactgatc
    13081 gacgccttag cgcaggaaac tgccgatatg ccgctgcatg tacagactga ccgggtaatt
    13141 aaagactccg gcctgcgtac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt
    13201 atcgaaaact tagaggaact agtgacggca acgcgccaat tcagctacaa cgaagaagac
    13261 gaagatttaa tgccgctgca ggcgttcctc tcccatgcgg cgctggaagc gggcgagggg
    13321 caggcggata cctggcagga tgcagtgcag ttgatgacgc tgcactcggc gaaaggcctg
    13381 gagttcccgc aggtgtttat cgtcggtatg gaagagggca tgttcccaag ccagatgtcg
    13441 ctggatgaag gcggacgtct ggaagaagaa cgccgtctgg cctacgttgg tgtaacccgc
    13501 gcgatgcaga aactgacgct gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt
    13561 taccatcgcc cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc
    13621 ctgcgcgcca cggtaagccg cccggtcagc catcagcgta tgggtacgcc gatggtcgag
    13681 aacgacagcg gctacaagct cggccagcgt gtacgccacg ctaagtttgg tgaaggcact
    13741 atcgtcaata tggaaggcag cggcgagcat agccgtttgc aggtggcatt ccagggccag
    13801 ggtattaaat ggctggtggc ggcatacgcc cggctggaga cggtataacg ttgcgggatg
    13861 cggtgcgcca caccgcatcc ggcatcagca ccttacttat cacatttctt cacatcaata
    13921 ttccagtcag caccagacgc aacctgatgt ttctgcgcga aattatccat attcagcgcc
    13981 acggaaacat tcaataagct gttcagatac accatcggct gtccttccgg cacatcgccg
    14041 aagcttgcga catacggcat tttccctgcg tatttttgcc gggaaccttc ggaaatggtc
    14101 acacacaacg tgtcgttgag tttgatcccc gcctgattca gcaactcgtc gctgatattg
    14161 ctccagacgt tgccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt
    14221 gttgcttttg ctttctgata agaaagttcg acaacctttg caggaagctc aggccccacc
    14281 tgctcgaacg tgatcgcccc ggaagccaga cgtgcaccgg tgtaggcata cacgtcacga
    14341 ccgtggaagg tatatgactt ttcagaacct ttcaggcggt tagctttttc gtcgatttcc
    14401 cggacgctct caatacccag cgattccgcc accaaagtga gcgtaccgtt atccggtgaa
    14461 acgaagtatt gaccgttttt ggttttcagc actatcgatt tacggtcagt gcctacgccc
    14521 ggatcgacca cactgacaaa taccgaacct tgcggccagt aactggcggt ctgatacagg
    14581 cggtaagcgc cttcccagat gttatacggt ggaatttcgt gtgttaaatc aaatatttta
    14641 agattatggt caacaccgaa tgcgacccct ttcatcgccg agacggcacc atctttcagg
    14701 ctaaaatcag tttgtaaaat caaagcgtta tccgcataag cggatgatga tcccagacac
    14761 gttaataaaa ataacgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat
    14821 gattttccag ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt acaatgctta
    14881 acctttccgc tcgacggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcccc
    14941 gcctttcata tacatatgtg aaatcgtgcc atcaagataa agcagctgct caacgttcag
    15001 tttcgctttg gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa
    15061 cacggcattc ccatgtttat taattccaac accgttacga attttacgtg aggcgacgtt
    15121 gggatgaata cgcggattaa ttacactgtt ttccatcaac attggccctg actgcaccgc
    15181 aaactgaatc tctttactgg ttttgaaggc atccagacga acgatgccga ctttatctcc
    15241 cgcgacataa aacacgccgc caggacggat aaagaaattc ccttcacctg aagcgagatt
    15301 taacgccacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc
    15361 atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattcatat ccgccagcag
    15421 agcatgtaaa gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc
    15481 tgtttgagga ttaacggtat acgcctgtac ggtcagcgtt ggatctgaga gtgcgcaatc
    15541 atcagcggca acagcaaaca agggaagtaa ggtgagggcg agaaaaatcc gtttgagatt
    15601 caaggtgatc attcctttac caatgagtag ccgatgcgcc attataggtc ctggatgtgg
    15661 gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg
    15721 ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg
    15781 atcactctaa gaggacattc gccttggaca cacccagtag atactggctc actatcctgt
    15841 catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg
    15901 acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt
    15961 tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc
    16021 gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt
    16081 gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa
    16141 tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca
    16201 cgtttctttg aagacgatga cggcctgcat attcactcct tcttcttctt tgaagatgcg
    16261 gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact
    16321 ctgcgtgagc gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg
    16381 atggtagacg gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag
    16441 ttggcagatg aaattgaaaa tatctatagc gacctggagc aattgagccg ggtgattatg
    16501 gaagggcatc agggcgatga gtacgacgag gcgctctcca ctctggcgga actggaagat
    16561 atcggctgga aagtgcgcct gtgtctgatg gatacccagc gcgcgctcaa cttcctggta
    16621 cgtaaagcac gcttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgagatatc
    16681 gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg
    16741 gcaatgggtt ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc
    16801 gtcgtgttcc tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg
    16861 ccagaactga agtggagttt cggctaccca ggcgcgatta tctttatgat cctcgcgggc
    16921 ctggcaccgt atctgtactt taagcgcaag aactggctgt aactatacga tgtcgctgtg
    16981 agtaagttag tgtgctgaca gcgacatttt agcagaggga atcgcagata acataattgt
    17041 tgtgaataat atccatgcca cgttgagttg gacgcttaat gactcacatc tcaccccttc
    17101 aattgctgca gagataatag aaaaaagtgt caaacccaat gaaatcaata caaagtagat
    17161 tgccttacat c
//
LOCUS       DAZFKY010000098        17121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFKY010000098 DAZFKY010000000
VERSION     DAZFKY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            148..393
                     /locus_tag="ACHKCL_002877"
     CDS             148..393
                     /locus_tag="ACHKCL_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO5763811.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            338..1258
                     /locus_tag="ACHKCL_002878"
                     /pseudo
     CDS             338..1258
                     /locus_tag="ACHKCL_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1273..1348)
                     /locus_tag="ACHKCL_002879"
     tRNA            complement(1273..1348)
                     /locus_tag="ACHKCL_002879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1313..1315),aa:Thr,seq:cgt)
     gene            complement(1463..2716)
                     /gene="proA"
                     /locus_tag="ACHKCL_002880"
     CDS             complement(1463..2716)
                     /gene="proA"
                     /locus_tag="ACHKCL_002880"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5763812.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2728..3831)
                     /gene="proB"
                     /locus_tag="ACHKCL_002881"
     CDS             complement(2728..3831)
                     /gene="proB"
                     /locus_tag="ACHKCL_002881"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5763813.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4119..5174
                     /gene="phoE"
                     /locus_tag="ACHKCL_002882"
     CDS             4119..5174
                     /gene="phoE"
                     /locus_tag="ACHKCL_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5763814.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5213..5614)
                     /gene="crl"
                     /locus_tag="ACHKCL_002883"
     CDS             complement(5213..5614)
                     /gene="crl"
                     /locus_tag="ACHKCL_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5763815.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5672..6916)
                     /gene="frsA"
                     /locus_tag="ACHKCL_002884"
     CDS             complement(5672..6916)
                     /gene="frsA"
                     /locus_tag="ACHKCL_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5763816.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7008..7466)
                     /gene="gpt"
                     /locus_tag="ACHKCL_002885"
     CDS             complement(7008..7466)
                     /gene="gpt"
                     /locus_tag="ACHKCL_002885"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5763817.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7727..9184
                     /gene="pepD"
                     /locus_tag="ACHKCL_002886"
     CDS             7727..9184
                     /gene="pepD"
                     /locus_tag="ACHKCL_002886"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5763818.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9241..9762)
                     /gene="prfH"
                     /locus_tag="ACHKCL_002887"
                     /pseudo
     CDS             complement(9241..9762)
                     /gene="prfH"
                     /locus_tag="ACHKCL_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(9761..9964)
                     /locus_tag="ACHKCL_002888"
                     /pseudo
     CDS             complement(9761..9964)
                     /locus_tag="ACHKCL_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(10283..10735)
                     /gene="yafP"
                     /locus_tag="ACHKCL_002889"
     CDS             complement(10283..10735)
                     /gene="yafP"
                     /locus_tag="ACHKCL_002889"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5763819.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(10745..11143)
                     /gene="yafO"
                     /locus_tag="ACHKCL_002890"
     CDS             complement(10745..11143)
                     /gene="yafO"
                     /locus_tag="ACHKCL_002890"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO5763820.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11146..11439)
                     /gene="yafN"
                     /locus_tag="ACHKCL_002891"
     CDS             complement(11146..11439)
                     /gene="yafN"
                     /locus_tag="ACHKCL_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO5763821.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(11491..12546)
                     /gene="dinB"
                     /locus_tag="ACHKCL_002892"
     CDS             complement(11491..12546)
                     /gene="dinB"
                     /locus_tag="ACHKCL_002892"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5763822.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVTPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12617..13402)
                     /gene="lafU"
                     /locus_tag="ACHKCL_002893"
     CDS             complement(12617..13402)
                     /gene="lafU"
                     /locus_tag="ACHKCL_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5763823.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            13374..15086
                     /locus_tag="ACHKCL_002894"
                     /pseudo
     CDS             13374..15086
                     /locus_tag="ACHKCL_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15231..15728)
                     /gene="rayT"
                     /locus_tag="ACHKCL_002895"
                     /pseudo
     CDS             complement(15231..15728)
                     /gene="rayT"
                     /locus_tag="ACHKCL_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(15904..16653)
                     /gene="yafL"
                     /locus_tag="ACHKCL_002896"
     CDS             complement(15904..16653)
                     /gene="yafL"
                     /locus_tag="ACHKCL_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5763824.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            16863..>17121
                     /gene="dinJ"
                     /locus_tag="ACHKCL_002897"
     CDS             16863..>17121
                     /gene="dinJ"
                     /locus_tag="ACHKCL_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFO5763825.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
BASE COUNT         4299 a         4396 c         4297 g         4129 t
ORIGIN      
        1 gtgataaatt atctctggcg gtgttgacac aaataccact agcggtgata ctaagcacat
       61 cagcaggacg cactaaccac catgaaggtg atgctcttaa aaattaagcc ctgaagaagg
      121 gcagcattca aagcagaagg ctttggtgtg tgtgatacga aacgaagcat tggccggaag
      181 tgcgaatccg gattagcagc caatgtgcca ttgcggggtg ttttcgttca ggactacgac
      241 tcccacacac aaccaaagct aactaacagg agaatccaga tggatgcaca aacacgccgc
      301 cgcgaacgtc gcgcagagaa acaggctcaa tggaaagcag atcggattct ggcttcaaca
      361 tttcgcagga atgcaactaa gagacattac tgaatcaaaa atttattcag caatgcagaa
      421 aatgacgaac cggcgtcatg aggaaaactg gaaactcagg gcagaagcat gcagaaaaaa
      481 agggaaacct gttccagaat acacgccaaa accagcgtcc gttgcaacga aggctacgca
      541 tctttcattt ataaaggccc tgctaagagc cgcagagcgt gaatggaaaa tgctcgataa
      601 ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg ctccgctggc tggagcccca
      661 tgaagcacaa aggctgattg atgaatgtcc ggagccatta aagtctgttg ttgaatttgc
      721 actggcaaca ggcttaagac gctcgaacat catcaacctt gaatggcaac aaatagatat
      781 gcagcgccgg gtggcatgga taaacccgga agagagtaaa tcaaaccgcg caattggcgt
      841 tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa atcggtaatc atcaccgttg
      901 ggtatttgtg tacaaggaaa gctgtaccaa accagacgga acgaaagcgc caacagtaag
      961 gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg ctgagacggg ctggtattga
     1021 tgatttcaga tttcacgact tgagacacac ctgggcaagt tggctggttc aagccggagt
     1081 cccgttgtca gtgttacagg aaatgggagg ctgggagtct atcgaaatgg ttcgtcgata
     1141 tgctcacctt gcacctaatc accttaccga acacgcacgg caaatagact cgatcctgaa
     1201 cccatcggtc ccaaatttgt cccagtcaaa aaataaggaa ggtactaatg atgtgtaact
     1261 tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca ttacgaatgc
     1321 gctgctctac caactgagct atatcggccc tgaaaggaca tgttcacgaa cgtgaatcac
     1381 ggtggacaag gttaaaacta accgggcgat gcgtcaatgg ccttgtgaat caaacggcta
     1441 cttttgcatc acccggtttt atttacgcac gaatggtgta atcaccaatg ccgatccact
     1501 tgtaagtggt cagtgcttcc agccccattg ggccacgcgc gtggagtttt tgtgtgctta
     1561 ccgccacttc cgcacccagt ccaaactggc cgccgtcggt aaaacgcgta gaggcgttaa
     1621 cgtaaacagc ggacgaatcc acttcgttaa caaaacgctg ggcgttgcgc atatcgcggg
     1681 tcaggatcgc atcggagtgt tgcgtgccgt gttcacgaat atgggcgatg gcatcgtcaa
     1741 gatcgctgac gattttgacg ttcaaatcta atgacagaaa ctcatcgtca tactcttcgg
     1801 ctttaaccgc caccaccttc gcagggcctg tctgcaactg cgccagcgca gatgcatctg
     1861 cgtgtaacgt cacgccgctt tccgccattt gtttgcttaa tgcgggcagg aagctatagg
     1921 cgatgttttt attcaccagc aacgtttcta ccgtattaca tgtgctcgga cgctgagttt
     1981 tcgcattgac gattactttc agggcttcag caatctctgc actttcatca acataaatat
     2041 ggcatacgcc tataccacct gtgatcaccg ggatcgtcga ctgctcgcgg cacagtttat
     2101 gcaaaccagc cccgccgcgc gggatcagca tgtcgatgta tttatccata cgcagcattt
     2161 cactgaccag cgcacggtca ggattatcaa tcgcctgcac ggcacccgcc ggtaagccac
     2221 aggatttcag ggcgtcctga atcaccacca ccgttgccgc gttagtgcga caggtttctt
     2281 tgccaccacg caggatcacc gcgttaccgg ttttcaggca cagcgaagcg acatcaaccg
     2341 tcacgttcgg gcgcgcttca taaatcacgc caataacccc cagcggtacg cgacgacgct
     2401 caagacgcag gccgctgtcc agtacgccgc catcgattac ctgccccact ggatcggcga
     2461 ggttgcacac ctgacgtaca tcgtcggcaa tgcctttcag ccgtgcgggc gtcagtgcca
     2521 gacggtcaag catcgcttcg ctaaggccat tggctcgcgc gtcagcaaca tcctgggcgt
     2581 tagcgttgag gatgatttcg ctttgtgctt ccagttcatc ggcgattttt tccagcacgc
     2641 gatttttttc gcggctggag agttgcgcta atttatacga ggcttgcttc gcggcaatgc
     2701 ccatttgttc cagcatcagc ctgctcctta acgggtgatc atgtcatcac ggtgaacggc
     2761 aaccgggccg tattcatatc ccagtattgc atcaatttct tgcgagtggt gtccggcaat
     2821 acggcgtaat gcatcgctgt tgtaacgact gacgccgtgg gcgatatcgc gaccttcgag
     2881 gttacaaatg cggatgactt caccacgcga gaagttgcca gtcacgcttt taatgccttt
     2941 cggcaacagg gagctgccgc gttcaagaat ggcggcggtt gccccttcat ctaccgtgat
     3001 ttcacccgca ggcggcgcac cgaaaatcca gcgtttacgg ttttcaagcg gagtcgcctg
     3061 ggcatggaac agcgtaccga cggaaatgcc ttccatcaca tcaccaataa cgcccggctt
     3121 gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa gccacgtcag cggcctgcaa
     3181 tttggtactc atgccgccag ttccgaggcc tgaaacgctg tcaccagcaa tcgcgcgcag
     3241 tgcgtcatca atgccgtaaa catctttaat cagttctgcc tgcggattgc tgcgcgggtc
     3301 agcggtatac aaaccttttt gatcggtcag taacaacagt ttatcggcac ccgccagaat
     3361 cgccgccagt gcagaaaggt tgtcgttatc gccgacttta atctctgccg tagcgacagc
     3421 atcgttctca ttgattaccg gaacgatatt gttatcgagc aacgcacgca gggtgtcgcg
     3481 ggcgttcagg aagcgttcac ggtcttccat atcagcacga gtcagcagca tttgcccgac
     3541 gtgaatgcca taaatcgaaa acagctgttc ccacagttga atcagtcgac tctgccctac
     3601 cgccgccagc agttgtttcg aggcgatagt cgctggcagt tccgggtaac ccaggtgctc
     3661 acgtccggcg gcgatcgcgc ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa
     3721 ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg ttcaggcggc gcgatccgcc
     3781 tgttagcaca ctggtgccga gttttaccac cagcgtctgg ctgtcactca tgattctctg
     3841 ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg
     3901 ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag cctagcggca
     3961 gttgtttacg cttttattac agatttaata aattaccaca ttttaagaat atcattaatc
     4021 tgtaatatat ctttaacaat ctcaggttaa aaactttcct gttttcaacg gggctctccc
     4081 gcagaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc actctggcat
     4141 tagtggtgat gggcattgtg gcatctgcat ccgtacaggc cgcagaaata tataacaaag
     4201 acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg agtgataacg
     4261 acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa acacaaatta
     4321 acgatcaact gactggttat ggtcgttggg aagcggagtt tgccggaaat aaagcggaga
     4381 gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg attgaagtat aaagatttgg
     4441 gttctttcga ctatggccgt aacctgggcg cgttgtatga cgtggaagcc tggaccgata
     4501 tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg accaaacgcg
     4561 ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat ggcctgaact
     4621 taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg
     4681 gcttcggcac gtcattgaca tatgactttg gcggcagcga tttcgccatt agtggggcct
     4741 ataccaactc agatcgcacc aacgagcaga acctgcaaag ccgtggcaca ggcaagcgtg
     4801 cagaagcttg ggctacaggt ctgaaatacg atgccaataa tatttatctg gcaacttttt
     4861 attctgaaac acgcaaaatg acgccaataa ctggcggctt tgccaataag acacagaact
     4921 ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg ggttatgtct
     4981 tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg
     5041 tcggggctac atattatttc aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc
     5101 aactggatag cgataacaaa ttgaatatta ataatgatga tattgtcgcg gttggcatga
     5161 cctatcagtt ttaatgatta ttgccggatg tgatgcatcc ggcacatttc actcacgccg
     5221 ttaacttcac cggctcgtca cgaaaatcat ccgccggttc caacttcaga ttcagcgtcg
     5281 tcagcagttc acgcagcttc tcgtgaaact cacgcagggt gtgctccagt cgttcaacca
     5341 cttcagtatc ttttaccgga acactcttcc agtcgcctgc tttatcgaac agaccaaact
     5401 ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc catccaccag ccccagaact
     5461 cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc
     5521 gattatcttc gcacttacct tcacgaatat acgggcctag tacggtaaat tttttgatca
     5581 atctgctctt cgggtgtcca ctcggtaacg tcattgctat ctcctgttgt gatgcaactg
     5641 ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa gcgtttttcg atccagtcgg
     5701 tgatttcctg aagacctttg tcaaaattcc gatacaccgg gttaaatggg atctctaata
     5761 atttaccgtc agcagatgat gaggtgatta agcgtgagtc ctcttccggg ctgaacggat
     5821 cgttcttcca gtagcctgat aacattggcg ttgggcatcg acgtcccagc aatccttgca
     5881 cttttaatga atagcgattc agctccacgc gcaacgcttc gtcggaagca tcatgcatcc
     5941 ccaaacgact cgccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat
     6001 cactcaacag ggtatgaact accggaccaa gacaggcaac cgctttcaga cgcggcgatt
     6061 caagatatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc
     6121 gagtgtgatc cacccacggt acgttaggca gagcctttaa gacgcgctga tgcaacaggc
     6181 aggagtcctg ggtgagcttc cattttgaag aaaagcccac cgacggcata tcaatagtca
     6241 gcatcgcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca
     6301 tcgcatccag accaccacac attaataccg tcgggaacgg gccatcgcct ttcggcatat
     6361 gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgt cgcatcgtgc
     6421 ccggtagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc gcttgctcgg
     6481 ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt gtacaacgta gccgcatgca
     6541 gccagtgacg accactgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca
     6601 tcgcctgggt ggcccactca taaatccagt tgccgccacg gtagccaatc accgtatcgt
     6661 ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga
     6721 tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt
     6781 gagggatggt tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc
     6841 ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca
     6901 ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaactcgc
     6961 ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag
     7021 attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt atcttgcggg
     7081 atatcaacaa cataatcatc aaccagcgga cgaccagccg gttttgcaaa gatggtgaca
     7141 aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt atccaccagg
     7201 tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg
     7261 cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt
     7321 tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc
     7381 cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc
     7441 caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa
     7501 aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc
     7561 agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt
     7621 ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct
     7681 gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc
     7741 tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc
     7801 gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt
     7861 ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta ccgcaggtat
     7921 ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc agaaaaataa
     7981 cgacaccgtg catgacttca cgaaagatcc tatccagcca tatattgatg gcgaatgggt
     8041 taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct ctgcactggc
     8101 ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga ccatgaccga
     8161 agaagccggt atggacggtg cgttcggctt acagagcaac tggttgcagg ctgatattct
     8221 gattaacacc gactccgaag aagaaggtga aatctacatg ggttgcgcgg ggggtatcga
     8281 cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg aaaccttcaa
     8341 gttaacctta aaaggtctga aaggcggtca ctccggcggg gaaatccacg ttggcctggg
     8401 taatgccaac aaactgctgg tgcgcttcct ggcgggtcat gcagaagaac tggacctgcg
     8461 tcttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac
     8521 cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtgaata cctatcagga
     8581 gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg gccttgttgc tggactctgt
     8641 agcgaacgat aaagctgccc tgattgcgaa atctcgcgat acctttattc gtctgctgaa
     8701 cgccaccccg aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg tcgaaacctc
     8761 cctgaacgtc ggtgtggtga ccatgactga caataacgta gaaattcact gtctgatccg
     8821 ttctctgatc gacagcggta aagactacgt ggtgagcatg ctggattcgc tgggtaaact
     8881 ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg acgctaattc
     8941 tccggtgatg catctggtac gtgaaaccta tcagcgcctg ttcaacaaga cgccgaacat
     9001 ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga
     9061 catggtttct atcgggccaa caatcaccgg tccacactct ccggatgagc aagttcacat
     9121 cgaaagcgta ggtcattact ggacactgct gactgaactg ctgaaagaaa ttccggcgaa
     9181 gtaattattt gatttgctgc cggatggcgt ttaatcgcct tccggcagtt tcatccttca
     9241 ttatccttcg ataaaagcca tccctgtgaa cgttcgtcgc gggttgccac gttcaatctg
     9301 gtgatggaac attcgccgct gcgatttcag cactgcgcta ttttcctgtt gctgctgctc
     9361 cagcttccag gcaatcagca atcgcgccag ccgtttgtta gcatgctggc tgcgttctga
     9421 ctgaaccttc acgctaatcc cggttgccag atgcgtggcg cgtaccgccg agtcggtttt
     9481 attgacatgt tgaccgcccg gccccgacgg acgcagcgtc tcataacgga ttgcatccga
     9541 ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga aaccagtttt tgcgcccatg
     9601 atgaggccga tacggactca gacaaatcca ctgaatagtg ccgcaccaac tttcgcttaa
     9661 cgcccatgcg ttgtcgccat ccagagaaat cagcgccgaa cgcagcgtgt cagagtagcg
     9721 gcccgtttct gtttccagca ccgttaccgc gacgtcttgt cgggtaaaaa cgaccaacag
     9781 agaagaacgc tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta
     9841 cgcgctgcat gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga
     9901 tccacaggtc atcagatgcg atggtaaata ccgcatcgga taagggacga atatatttgc
     9961 ccatgtacca ttccatttat actcgtcata cttcaagttg catgtactgc gactgcgttc
    10021 gttcacccca gtcacttact tatgtaagct cctggggatt cactcgcttg tcgccttcct
    10081 gcaactcgaa ttatttagag tatataaata taaaaatcag gaaatggcag acgaatccag
    10141 ggcaagaaaa aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
    10201 gtcatgattt acctccttgc agtttgtgga ataagaaaac gcgcacatac caggcgggcg
    10261 ttattttcat tgcaagctgg atttaatgtt gcggtttata tcgcatagaa aaattagtaa
    10321 accacgctcc ccggcattca acgcgctgct gcttaactgt ctgaaaacca taacgttcaa
    10381 aaaagggttt tgcggttatg ctggcgtcca ccgtaagttc ggattcagac ttaatccaag
    10441 gttttaacaa agcgctggca accccacggc gggtgtattc agggtcaaca aataacatat
    10501 cgatataatg ttcaatgcgg gaaataaaac caaccggttg tgcattaatg actgcaaccc
    10561 gcacttgtga tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg
    10621 caatttgttg tggtgaataa tgctgactgg cggtcatcat aaccgctcta atgaaaatag
    10681 cgcatagttg ctgaaaatcg ccaggctgat agtttcttat ttgtatgtta ttcataatat
    10741 aaattcaaaa acgcatgcga aacgcttctg ccattttccc aattttatgc atttggttgt
    10801 tatctcgagc cagtttatga ggttcaggtt tcagaatggc aatgagcaac catgcttgct
    10861 catcaaacgc accctgacaa tataccaaat gcgattcgtc attcgttctg ctgaattggc
    10921 gcaactgtgg cggaaatgga ttattcacat ttgccagatg aatatgagca actcgctcaa
    10981 atttgattaa tggccaagta aacgagtcgt cgtagagtgc atcgcgacca aatatatctg
    11041 gcaaaacacc gtcacgctta taggaaataa aatccgccgt taacgcatca agctcctctg
    11101 ctgtaagttg caggcgaata agttttgttt tgaatacccg catccttatt ccttaaagtc
    11161 attgaaatca tcatccgtca tatcattaag atatttttca gctcggcgtg taatttcctc
    11221 taatcttgca gcacttggac ggaagcgcgc ctttatgggc tttttctcct cttgttcagc
    11281 aagcatgtcc attaacgctt cgaatgcgct ggcacttaag agatatcctg cggggcgatt
    11341 attagaaaga accgcaaccg gttgatcaat aaagtattta gctgggtttt tacgtaattc
    11401 agtgatattg accgattttt cagcgagaat tcgatgcata cagtgatccc ctcataataa
    11461 tgcacaccag aatatacata atagtataca tcataatccc agcaccagtt gtctttccat
    11521 ttgagggtca agcaacgtca catgcagccc caccagtcgc acaccgcgcc cgccgcggcg
    11581 ttcatcccag gttttacgcg cggtggcgat taagtcagct ttattcagcc gcggccagac
    11641 gtgctcctgg gtggtttgct gaaaatcgtc gaactttaat ttcaccccct ggcgagcaat
    11701 cagtaaatca ggttttacct ttgccagacg gcgttcaagt tccggataca gccgctcgat
    11761 aatcgcttca cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac
    11821 ggattttcgt aaccgttcgc tgttaacgtc gcgctcgtca atcccctgac tacgctccca
    11881 caaaatgcgg ccaaatttgc caaagcgttt aagcagaatc accagatcac acttttgtac
    11941 atcaccgcag gtccgcagcc ccatcgcttc cagctttgcc gctgagactt tgccaacacc
    12001 gggaattttt gccagcggta aggtttgcag aaatgccgaa acttctgccg gcgtaatcac
    12061 aaactggccg ttgggtttat tcatgtcgga ggcgattttg gcgagaaact ttactggtgt
    12121 cacgcccgca gacgccgtca gttgcagctc gctgaaaatt gtctggcgga tttcctgggc
    12181 gatgagggtc gcagaaccgt ggcaatggac gctatcagtg acatcgaggt aggcttcatc
    12241 cagtgacaac ggttcaatac gcgaggtgta gcgcgagaag atttcacgga tatgatttga
    12301 ggcttctttg taggcgtcaa agcgccccgg aagcaaggtg agatgtgggc ataatttgag
    12361 cgccatccct gtcggcatag cgctgcgtac gccaaattta cgcgcgggat aattggcggt
    12421 gctgatcacc ccccgacgtt cgcggctgcc gccaatagca atagggatat cgcgcagggc
    12481 gggattgtcg cgcatctcca ctgcggcgaa aaagcagtcc atatccacat gaatgatttt
    12541 acgcattact cacctctcaa cactggtaaa gtatacagtg atttcagggt ttgagaaatg
    12601 cgtaaagatt cagcatttag cgcgttcgct gcgaaagcac ctgctgctta tccagctttt
    12661 gcaccagcgg ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg
    12721 acgcactttt ggtcagcacc ataatctcaa tgcgtcggtt gcccgcgctt tgcggatttt
    12781 tcgcgtccag cagcatctgg tccgccattg cgcttacctg catcacttta tcttccggca
    12841 ttccggcctc ttccagcacc cgacgagctg aaagcgcgcg atcgcccgaa aggttccagt
    12901 tgttgtagat attgtttttg taggccatcg catcggtatg cccggtaata ataattttgt
    12961 tatcgagcga gtcgaacact ggcgcgagct ccaccagcaa agttttaaag aacggcataa
    13021 tctgcgcgct gccgcgttcg aacatattgc gattctggtc gtctttaatc agcacgcgta
    13081 atccctgcgg gacaatctcc atctccagat tcgcttccat atgcgcatca cgggcaatgg
    13141 tgttgatact ggttgccagc tcgccaagct cggtggcgga tttcttcttc agcagcgccg
    13201 ttttctcatt cacatcacgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag
    13261 gttttggcgg atgagacggg ctgattttat ttaacggcga cagcccacca ccgttaaaaa
    13321 tcgactgccc gtgcagcgcg gcgataatgc tttcacgctc ggatttactg acgctgttga
    13381 caatcaactt tatcgtcgtc accaaagggg ccgagcgtat ttccgaggtt tctgcccgct
    13441 ttaccctgga tgcgatgccc ggcaaacaga tggcgattga cgccgatctt aacgccggat
    13501 tgatcaacca ggcacaggcg caaacccggc gtaaagatgt tgccagtgag gccgatttct
    13561 acggcgcgat ggacggggca tcgaagtttg tgcgcgggga tgccatcgcc gggatgatga
    13621 ttctggcgat taacctgatc ggcggcgtct gtatcgggat atttaaatac aacctgagcg
    13681 ccgacgccgc cttccagcaa tatgtgctga tgaccatcgg cgatgggctg gtggcgcaga
    13741 tcccttccct cctgctctcc accgcagcgg cgattatcgt cacccgcgtc agcgacaacg
    13801 gcgatatcgc ccatgacgtg cgccaccagc tgctggcaag cccgtcggtg ctctacaccg
    13861 ccaccgggat tatgtttgtg ctggcggtgg tgccgggaat gccgcacctg ccgtttttgc
    13921 tgttcagcgc cctgctgggt tttaccggct ggcggatgag caaacagccg caggcggcgg
    13981 aggcggaaga gaaaagcctc gaaacgctga cccgcaccat cactgaaacc agcgagcaac
    14041 aggtcagttg ggaaaccatt ccgcttatcg agcctatcag tttaagcctc ggctacaagc
    14101 tggtggcgct ggtcgataaa gcgcagggca acccgctcac ccagcgtatt cgcggcgtac
    14161 ggcaggtgat atccgacggt aacggcgtgc tgctgccgga gatccgcatt cgggaaaact
    14221 tccgcctcaa gcccagccag tacgccattt tcatcaacgg cattaaggcc gatgaagcgg
    14281 atattccggc ggataaactg atggcgctgc cctccagcga aacctacggc gagattgacg
    14341 gcgtgctggg gaacgacccg gcgtacggga tgccggtgac ctggatccag cccgcgcaga
    14401 aggcgaaggc gctgaatatg gggtatcagg tgatcgacag cgccagcgtg attgccacgc
    14461 atgtgaacaa gattgtgcgc agctatattc ctgatttgtt taattatgat gacatcacgc
    14521 agctgcataa ccgcctgtcg tcgatggccc cgcgcctggc ggaagatttg agcgcggcgc
    14581 tgaattacag ccagttgctg aaagtgtacc gtgcgctgct gaccgaaggc gtttccctgc
    14641 gcgatatcgt caccatcgcc accgtgctgg tcgccagtag cgcggtgact aaagatcata
    14701 ttctgctggc ggccgatgtg cgcctggcgc tgcggcgcag cattacccat ccgttcgttc
    14761 gcaagcagga gctgacggtg tatacgctga ataatgagct ggaaaatctg ctgactaacg
    14821 tagtgaatca ggcgcaacag ggcggtaaag tgatgctcga cagcgtgcca gtggacccga
    14881 atatgctcaa ccagttccag agcacgatgc cgcaggtgaa agagcagatg aaagcggcgg
    14941 ggaaagaccc ggtgctgctg gtgccgccgc agctgcgccc tttgctggcg cgctatgcaa
    15001 ggttgtttgc gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt
    15061 tgaagataat gggggcgttg agttgattgg cctttaaggg gcgcatccgg cattggtgca
    15121 caattgccgg atgcggcgtg aacgccttat ccggcctacg gttgagtgca gttttgtagg
    15181 catgataaga cgcgccagcg tcgcatcagg catccggcac cagcgcacaa ttatgaaatg
    15241 atacgctccc cggcactaaa atccgttatg tctcccgccc aatcaatggg atataaccct
    15301 cgcgccacat cgcgatggaa cgttgagaat ggccaatcac tcacttgctt tacccagcca
    15361 tgttttactg gatttatata aatataatca acatgatggc gatagtcttt ggtattgcgg
    15421 atggtgtgct cctaaaaacg tggttgccag atatttttca atccacaagc atgggtgaac
    15481 tgctttttaa tctcccgcca gcgggaggaa aaatcatcat cgctttcggg taatgtccat
    15541 atgcaatgca tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc
    15601 ttaacgttaa taatggcgtt acggagcatc tggaactgag cggtcagaag gtggcttcga
    15661 cgatttcgta ggttaaccgt gaaaaaccag gttccccctt tgatgtaata ccgacgatat
    15721 tcagacatgg caactccttt ttctcaacgg taagaagaaa aggaaaggtc ggcaatgagg
    15781 tgatggaata acgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg
    15841 cgtcagcgtc gtatcaggca tctgcgcaca actgccggat gcggcgtgaa tgccttatcc
    15901 gtcctacaaa atcgtctctt ccgtcaaaat ccggcgcgcg cctaaaaagt gatcctgcca
    15961 gaaaggttca gctaaccgac ttacccgaat ggtttccccg gtgcgcggtg actcaataaa
    16021 ctgaccgtcg cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa
    16081 aaacagcaaa tctccccggc gcaggtcgtt attcgccaca atcgttgccc gatggtagtg
    16141 atacatctca ttagccgtac gcgggagctt cgcctcgagg atcttgttgt aggcataaaa
    16201 aaccaacccg ctacagtcaa agcctttatc aggccgcgtg ccgccccaga cgtaaggctt
    16261 gccaagctgc tgctccaggc ggtgaatcgc cacttcggta atgttatgca gatggtcgct
    16321 gcttaaaaag tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg
    16381 tgcaggaaac cattctggat gctgttttat ctgttcgcgg ttgtgctggc gtagcgccct
    16441 tctgctttcg gcattgcctt ccacaatata gctgacctgc tttttcagat gggtttggta
    16501 ctgttttaat aaacgcgcac ggttttgcat ccgctggcgg gcggcgtagc tgaaagagat
    16561 atgtgatgtg gtggcgctgg caaatgaaga aaacggcaaa caaatcaaca gtaatcccga
    16621 caatacaaag gatggaatcg acggcaagga catactcaat aatggctggt catgcattga
    16681 tgttacagtg tatcccggcc cgtacctgaa caaacttaat aaaccattat tataaaatta
    16741 ataaatatat ttcagttaat tatgtaaaaa catccgttta ttaaattact catagattgt
    16801 cgctgttgct catttgagct acaattcaag ctgaataaat atacagcata ggagatatcc
    16861 caatggctgc taacgcattt gttcgcgccc gaatcgatga agatctgaag aatcaggcgg
    16921 cggacgtact ggccgggatg gggctgacca tctctgacct ggttcgcata accctcacaa
    16981 aggtcgcgcg tgaaaaggca ttgccgtttg atttacgcga gcctaatcaa ttaaccattc
    17041 aatcaatcaa aaacagcgaa gctggcgttg atgttcataa ggccaaagac gccgatgatt
    17101 tatttgataa attaggagtt t
//
LOCUS       DAZFKY010000099        17078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFKY010000099 DAZFKY010000000
VERSION     DAZFKY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1545
                     /locus_tag="ACHKCL_002898"
     CDS             <1..1545
                     /locus_tag="ACHKCL_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO5763826.1"
                     /translation="EIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQ
                     FSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSL
                     ISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIA
                     EKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPL
                     QGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELF
                     LVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAM
                     GEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETV
                     IERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGT
                     AGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKS
                     GKLVKE"
     gene            1553..2881
                     /locus_tag="ACHKCL_002899"
     CDS             1553..2881
                     /locus_tag="ACHKCL_002899"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5763827.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2951..3280)
                     /locus_tag="ACHKCL_002900"
     CDS             complement(2951..3280)
                     /locus_tag="ACHKCL_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5763828.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3270..3656)
                     /locus_tag="ACHKCL_002901"
     CDS             complement(3270..3656)
                     /locus_tag="ACHKCL_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5763829.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(3882..5207)
                     /gene="rimO"
                     /locus_tag="ACHKCL_002902"
     CDS             complement(3882..5207)
                     /gene="rimO"
                     /locus_tag="ACHKCL_002902"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5763830.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5420..5803
                     /gene="bssR"
                     /locus_tag="ACHKCL_002903"
     CDS             5420..5803
                     /gene="bssR"
                     /locus_tag="ACHKCL_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5763831.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5914..7029
                     /gene="yliI"
                     /locus_tag="ACHKCL_002904"
     CDS             5914..7029
                     /gene="yliI"
                     /locus_tag="ACHKCL_002904"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5763832.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7026..7652)
                     /gene="gstB"
                     /locus_tag="ACHKCL_002905"
     CDS             complement(7026..7652)
                     /gene="gstB"
                     /locus_tag="ACHKCL_002905"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5763833.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7898..9100
                     /gene="dacC"
                     /locus_tag="ACHKCL_002906"
     CDS             7898..9100
                     /gene="dacC"
                     /locus_tag="ACHKCL_002906"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5763834.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9147..9905)
                     /gene="deoR"
                     /locus_tag="ACHKCL_002907"
     CDS             complement(9147..9905)
                     /gene="deoR"
                     /locus_tag="ACHKCL_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5763835.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9963..10559)
                     /gene="ybjG"
                     /locus_tag="ACHKCL_002908"
     CDS             complement(9963..10559)
                     /gene="ybjG"
                     /locus_tag="ACHKCL_002908"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5763836.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10844..12076
                     /gene="mdfA"
                     /locus_tag="ACHKCL_002909"
     CDS             10844..12076
                     /gene="mdfA"
                     /locus_tag="ACHKCL_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5763837.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12117..12401)
                     /gene="ybjH"
                     /locus_tag="ACHKCL_002910"
     CDS             complement(12117..12401)
                     /gene="ybjH"
                     /locus_tag="ACHKCL_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5763838.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12487..13302)
                     /gene="ybjI"
                     /locus_tag="ACHKCL_002911"
     CDS             complement(12487..13302)
                     /gene="ybjI"
                     /locus_tag="ACHKCL_002911"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5763839.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13302..14510)
                     /locus_tag="ACHKCL_002912"
     CDS             complement(13302..14510)
                     /locus_tag="ACHKCL_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763840.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14594..15130
                     /gene="rcdA"
                     /locus_tag="ACHKCL_002913"
     CDS             14594..15130
                     /gene="rcdA"
                     /locus_tag="ACHKCL_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5763841.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15305..16990)
                     /locus_tag="ACHKCL_002914"
     CDS             complement(15305..16990)
                     /locus_tag="ACHKCL_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5763842.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         4409 a         4097 c         4244 g         4328 t
ORIGIN      
        1 gaaatctatt tttattatcc gggcggtaat ttattgaacg catcagataa acttttttat
       61 ctgccgtttg cgttcattat tatcgtattg ctggtggttt atttaatgac cactcgtgtg
      121 ttccgtcggc aattttctga aatgacagag ctgattaata cgctggcgtt tttgcctgac
      181 tcaacggatc agatcgaggc tctgaaaatt cgcgaaggcg atgcgaaaga gattatcagc
      241 atcaaaaatt cgatcgcgga aatgaaagat gccgaaattg aacggtcaaa taaattgctc
      301 tcactgatct cttacgatca ggaaagtggt tttattaaaa atatggcgat tattgagtcc
      361 aacaataatc agtatctggc tgtggggatc atcaaactgt gtggtctgga agccgtggaa
      421 gctgtgtttg gtgttgatga acgcaataaa atcgtcagga aattgtgtca gcgaattgct
      481 gagaaatatg cacaatgctg cgatatcgtg acattcaatg ccgatctcta tttacttctg
      541 tgtcgggaaa atgtacagac atttacccgt aaaatagcga tggtaaacga ttttgacagc
      601 agctttggct accgcaatct gcgcatccat aagtctgcca tttgtgaacc tttgcagggg
      661 gaaaacgcct ggagttacgc agaaaaactg aaactggcga tttccagtat ccgtgaccat
      721 atgttctccg agtttatttt ctgtgatgac gcgaaactca acgaaataga agagaatatc
      781 tggattgcgc gtaatattcg ccatgcaatg gaaattggcg aactattcct cgtctatcaa
      841 ccgatcgttg atattaacac ccgcgccatt ctgggcgcgg aggcgttgtg ccgttgggtg
      901 tctgcggagc gggggatcat ttcaccgctg aagttcatta ccattgctga agatatcggg
      961 tttatcaatg agctgggtta tcagattatt aaaacggcaa tgggtgaatt cagacatttt
     1021 agtcagcgtg catcgctgaa ggatgatttc ttactgcata ttaatgtttc gccctggcag
     1081 ttaaacgaac cgcactttca tgagcgtttt accaccatca tgaaagaaaa tggcctgaag
     1141 gcgaacagcc tctgtgttga gatcactgaa accgtgatcg agcgaattaa tgaacatttt
     1201 tatctcaata ttgaacaact gcgtaaacaa gggttacgga tatcgattga tgactttggc
     1261 accggtttgt caaacctgaa acgtttttat gaaattaatc cagacagcat aaaggtggac
     1321 tcgcaattta ccggcgatat tttcggtact gcgggaaaaa ttgtgcgcat tattttcgac
     1381 ctggcacgct ataaccggat cccggtgatt gcggaaggcg tagagagcga agacgttgcg
     1441 cgcgaattaa tcaaattagg atgtgttcag gctcaggggt atctgtatca gaaacccatg
     1501 ccattctccg cctgggataa aagtggaaaa ttagtaaaag agtagtttac gtatgtccag
     1561 aatcaataag ttcgtactta cagtcagtct gctgattttt atcatgattt cagcagttgc
     1621 ctgcgggatc tacactcaaa tggtaaagga acgggtgtat agcctgaaac agtccgttat
     1681 tgatactgct tttgcggtgg caaatattgc tgaatatcgg cgtagcgtgg caattgatct
     1741 tatcaacacg ctaaatccca cggaggaaca gctgttggtt ggcttacgca cagcttacgc
     1801 cgactcggtt tcaccctctt atttgtacga tgtcggtcct tatctgattt ccagtgacga
     1861 atgtattcag gtaaaggagt tcgagaaaaa ttattgtgca gatattatgc aggttgtgaa
     1921 gtatcgacat gtcaaaaata cagggtttat ctcttttgac ggtaaaacct tcgtctatta
     1981 cctctatccg gtaactcaca atcgtagtct gatatttttg cttggtctgg agcgtttttc
     2041 tttactgtca aaatcgctgg cgatggacag cgagaacctg atgttctctc tgtttaagaa
     2101 cggtaaaccg gtgaccggtg atgaatataa tgctaaaaac gccatcttca ccgtttcgga
     2161 agcgatggag cacttcgcct atttgccgac cggattgtac gtatttgcgt ataaaaaaga
     2221 tgtttatttg cgggtctgta cattgattat tttctttgcc gcattggtgg cagtgatatc
     2281 gggtgccagt tgcctctatc tggtacgcag agtgattaat cgtggtattg tggagaaaga
     2341 agccattatt aataaccatt ttgaacgcgt actggatggc gggcttttct tttcggctgc
     2401 cgatgtcaaa aaactctaca gtatgtataa ctcggcgttc ctggacgacc tgaccaaagc
     2461 aatgggtaga aaatcctttg acgaagattt aaaagcgctg ccggaaaaag gcggttattt
     2521 gtgcctgttt gacgtcgata aattcaaaaa tattaacgac accttcggtc atttgctggg
     2581 cgatgaagtg ttgatgaaag tggtgaaaat ccttaaatca cagatcccgg tagataaagg
     2641 taaagtctac cgcttcggcg gtgacgaatt tgcggtgatt tatactggcg gcacactgga
     2701 agagttacta tcgattctaa aagaaatcgt tcatttccag gtgggaagca ttaatttaag
     2761 caccagtatc ggtgtagcgc attcaaatga atgtactacc gtcgaacgct tgaaaatgct
     2821 ggcggatgag cggctgtata agagtaagaa aaacggcagg gctcagatta gctggcagta
     2881 gaattagaat agtgttagta cccctgaatc aaggggtact aatgaatgtg gtaaatcatt
     2941 tgcaaacatt ttactttgca aaaacttccg gcccttttat tgccaatgta ttcaaaatac
     3001 gtagtgccgg gccgctcggt tttatttcat tacgttccca tttcgagaca ctttcttttg
     3061 acattcccat tgtatgtgcc agcacggatt gtgacatgcc gtatcgggtg cggatggctt
     3121 tgatctcttc acccgtcatt gatttgactt tggctatacg ggcgctgact tcacgggctt
     3181 tgaccatgac ggcaatggca tcaacctcac tatcagacat caggcccagt gcattcagtt
     3241 cctttgcatc ttccagcatc atttgtaatt tatcgctcat ttttcacctc aatcaagaag
     3301 ccagtttcga tcaaatcctc tatttgctta ttgctaaagg ccagaaatgc atttgccatt
     3361 tttttgtagg ctgccagttc atcatcttcg atttctttgc ttcctttact gcttagacca
     3421 ctcttggacc aaccatcata aaaaaacaca tgatttgctg atttaaaaaa gataattgtt
     3481 ctggctccac cactctttcc ttgttggagg ggtaaccgtt ttttgatgac accgctacct
     3541 aaatcagcct cccatatgcc ttgcatcact tcttcagcgg cagccatgag aactttatcg
     3601 tgaataccaa gttttttcgt gctttggctg aatcgccttg ccttatacac acccatacat
     3661 ccatccttgt ggtaatgttt actatagtaa ccattacgct tatttcaagt aattgcgatc
     3721 aaaactttaa tttggtgtga aaaagtgaga gttgcctcga gtacgattca tttttctcga
     3781 tttatgcgtc aggggatttt gtgcgtaagg gtaggacatc gggaagggta gtagaggcag
     3841 atcgaagaac aatatccttg ttagatctgc ctttagatta attaaacccg gctaccccac
     3901 aaatcgtact catcggcgtg ctcgactttc acacgcagga tatcacccgg cttaacgttg
     3961 gtttcaccat tgagataaac cgcaccgtcg atttccggtg catctgccat gctgcgacca
     4021 atcgcgcctt cttcgtccac ttcgtcgata atcaccagaa tttcacggcc cactttctct
     4081 tgcaggcgct cggcggaaat ctgctgctgc aactgcatga aacggttcca gcgttcttct
     4141 ttcacttctt ccggaacctg gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg
     4201 tatttaaagc agccaacgcg atccagacgc gcttctttca ggaagtcgag tagcatctgg
     4261 aaatcttctt ccgtctcgcc agggaagccg acaataaagg ttgagcgtag ggtcagttcc
     4321 gggcagattt cgcgccactg tttgatgcgc gccagttggc gatctacaga acccggacgc
     4381 ttcatcagtt tgagaatgcg cgggctggcg tgctgcaacg gaatgtccag atacggcagg
     4441 attttgcctt ctgccatcag tgggatgacg tcgtccacat gcggataagg gtaaacgtag
     4501 tgcagacgtg tccagatccc cagtttcgat aactgttcgc acaggctgac catgctggtt
     4561 tttaccggct cgccgttgtg gaagccagta cgatgtttaa catcaacgcc ataggcggaa
     4621 gtatcctgcg agatcaccag aatctcttta acgcccgcat ctaccagacg tttcgcttca
     4681 cttaatactt cgccaatcgg acggctcacc aggtcgccgc gcatagacgg aataatgcag
     4741 aaggtgcagc ggtgattaca gccttcagaa attttcagat aggcataatg acgcggcgtc
     4801 agtttgacac cttgttctgg caccaggctc aggaatgggt tgtgtttcgg ttttggcacg
     4861 tagtgatgaa cgtgctccag aacctgctca tagctatgag gcccggtgat ttccagcact
     4921 ttcgggtgga cttcgcggat ctgatcttct tttgccccca gacaaccggt cacaattacc
     4981 ttgccgtttt cattcaacgc ttcaccaatg gcttccagtg attcttgtac cgcgctgtca
     5041 ataaagccgc aggtgttgac gatcaccatg tccgcatcgt catagctcgg taccacgtca
     5101 taaccttcag tgcggagttc ggtgagaata cgctctgaat cgacaaggtt tttcggacag
     5161 ccaagggaaa caaagccgat tttcggctgg ggagttactt tgctcatagc ttaaaaaata
     5221 ttcagttaca ggaaaggtca gggcagggat tctacagagt tctggataaa atttgtatcg
     5281 caatctcatt cgctggcgga ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa
     5341 ttgatcctgg acagcattat gctcaaaaaa tagccatact atttaattgc aacaaggctg
     5401 gaaagaggag gatcgaagta tgttcgttga cagacagcga atcgatctgc tgaaccggtt
     5461 gatcgacgca cgcgttgacc tcgccgcata cgtgcaactg aggaaggcaa aaggatacat
     5521 gtccgtcagc gaaagcaatc atctacgaga taactttttt aaactgaatc gcgaactgca
     5581 cgataaatcg ctgcggttga atcttcatct ggatcaggaa gagtggagtg ctcttcatca
     5641 tgctgaagaa gcattagcga cagccgcagt atgtttgatg agtgggcacc atgattgccc
     5701 gactgttatt accgtcaacg ccgataagct tgaaaattgt ctgatgagct taacgctgag
     5761 tatccagagc ctgcagaagc acgccatgct tgagaaggcc tgaaaactaa gggggagaaa
     5821 gcgtctcccc cttcatgttt aagtttttgt aaaaatgaat ttgttatctc ctccactgac
     5881 tacgctttaa gccagagtca atccggaggc gttatgcatc gacaattctt tttccttgtg
     5941 ccccttattt gtctttcttc cgctctctgg gcggctcctg caacggtaaa tgtcgaagta
     6001 ctgcaagaca aactcgacca tccctgggca ctggcctttt tacccgataa ccacggtatg
     6061 ttaatcactc tgcgcggcgg cgagttgcgt cactggcaag caggaaaagg attatctgcg
     6121 ccgctttccg gagttccgga cgtttgggcg cacgggcagg gcggcctgct ggacgtggtt
     6181 ttagcgcctg attttgctca gtctcgccgc atctggttaa gttattccga agttggcgat
     6241 gatggcaaag ccggaactgc tgtggggtat ggccgcttaa gtgatgatct ctcaaaagtg
     6301 accgactttc gcaccgtctt tcgccagatg ccaaaactgt ctaccggcaa ccattttggc
     6361 gggcggctgg tattcgacgg taaaggttat ctttttattg ctctgggcga aaacaatcag
     6421 cgcccgacgg cgcaggatct ggataaatta cagggcaaac tggtgcgtct gaccgaccag
     6481 ggcgaaatcc cggatgataa tccttttata aaggaatccg gtgcgcgcgc cgagatctgg
     6541 tcttatggca ttcgtaatcc gcaaggaatg gcgatgaatc cgtggagtaa tgcactgtgg
     6601 ctgaatgaac atggcccgcg cggtggtgat gaaattaata tcccgcaaaa aggcaaaaac
     6661 tacggctggc cgctggcaac ctggggaatc aactattcag gctttaagat accggaagcg
     6721 aaaggggaga tcgtcgccgg gaccgagcaa cctgtttttt actggaaaga ttcgcccgcc
     6781 gtgagcggca tggccttcta taacagcgat aaattccccc agtggcagca aaaattattt
     6841 attggcgcgc tgaaagataa agatgtcatt gtgatgagcg tcaacggcga caaagtaaca
     6901 gaagatggcc gtattttaac ggacagaggg cagcgaattc gtgatgttcg cactggaccc
     6961 gacggttatt tatacgttct caccgacgag tccagtgggg aattacttaa agttagccca
     7021 cgcaattagc taacgggaat catcaccact ttgcgcactg cggggcgttc agtgagttgc
     7081 tgataccagc gttgcagatt tggacgcggt gtccaggtca ggccaacatt aaacaaattg
     7141 tagatgaacg gtgcgatagc gatatcgccc acaccaaact cgtcgccaga gaaccatttt
     7201 acttttgcca gttccgcatc gagcagggca aacagggcgt cgcactcttt gcaactggca
     7261 tcaatggcgg cctgatcgcg ctcttccggt ggtgttctga ctaatcccat caggatcccg
     7321 cgatgagcat tactgagtgt ctggtttgcc cagtccatcc atttttctgc ttccgcacga
     7381 cgtgccggtg agtcgatcca caggcgtttt tgcccgtact gtgccgccag atagcggaca
     7441 atggcgtttg attcccaaag aatgagatca ctttcgtcgt cacgcaacaa cggcaccagc
     7501 ccgttagggt tcatcgccag aaaatcagca tcgtgattta tcccaaactc acggcccgcg
     7561 agaatttgct cataaggtag ttccagttct tcgagcgtca gcaatacttt ttttacgttg
     7621 gttgaattat tccgacccca cagcgtaatc atactcaccc cttttccgac aggcagccac
     7681 aggctgaaca ggactccgat ggtgaagtaa agttaacttt taagcaacag ctggcaaaaa
     7741 aatgacacca gaagcagcga agcggcagga tattgcataa actttaaaaa ctttaccaac
     7801 ttacggtttc tttaagtttg tgtgtgcgtt attaatcacc gaacttatca tacggcgata
     7861 taacgtattt ttttgaatgg atactcgggt ggcatttatg acgcaatact cctctctcct
     7921 tcgtggtctt gcagcgggtt ctgcattttt attccttttt gccccaacgg cattcgcggc
     7981 cgaacaaacc gttgaagcgc cgagcgtgga tgcgcgtgca tggattttaa tggattacgc
     8041 cagcggtaaa gtgctggcag aaggcaatgc ggatgagaaa ctggatcccg cgagcctgac
     8101 taaaatcatg accagctatg tggttgggca ggcgcttaag gccgataaga ttaaactcac
     8161 cgatatggtg acggtcggta aagatgcctg ggcgacggga aatccggcac tgcgtggttc
     8221 atcggtaatg ttcctcaaac cgggcgatca ggtttcggtg gcagacttga acaaaggtgt
     8281 gattatccag tccggtaatg acgcctgtat tgcgctggcc gattacgttg ccgggagcca
     8341 ggagtcattt attggtttga tgaatggtta tgccaaaaaa ctggggctga ccaacactac
     8401 cttccagacg gtgcacggtc tggatgcgcc ggggcagttc agtaccgcgc gcgatatggc
     8461 attgctgggt aaagcattga tccacgatgt gccggaagag tacgccattc ataaagagaa
     8521 agagttcacc ttcaacaaaa ttcgtcagcc taaccgtaac cgtctgctgt ggagcagcaa
     8581 tctgaatgtt gatggcatga agacaggaac cacggcaggc gcgggatata atctggttgc
     8641 ttcggctacc cagggcgata tgcgtttaat ctccgtagtg ctgggggcga aaaccgaccg
     8701 tatccgtttt aatgagtctg agaaattatt gacctggggt ttccgcttct ttgaaaccgt
     8761 gacgccaatt aaacctgatg ccacctttgt gactcagcgc gtctggtttg gtgataagag
     8821 cgaagtgaat ctcggggcag gcgaagcggg ctcagtgacc ataccgcgtg ggcagctgaa
     8881 aaacctgaag gcgagctaca cgttaacgga accgcagctt accgcaccgc tgaaaaaagg
     8941 tcaggttgtc gggaccattg atttccagct taacggtaaa tccattgagc agcgtccgct
     9001 gatcgtgatg gaaaatgtgg aagagggcgg attctttggt cggatgtggg atttcgtgat
     9061 gatgaaattc catcagtggt tcggcagctg gttctcttaa tcttctgata accggatggc
     9121 gcgaaacgtc atccggttat acgtcattaa tacatcaact taatacgctg cgtctgcgcg
     9181 tacttcacat actcatcttc cgggcaacaa tcgctcacca caatatcaaa gcgtttcagg
     9241 tcacccatgc gcgccggacg caccttgcca aatttactgt ggtcaacaac cagcacatgc
     9301 ttttgcgcca tcgacatggc ccagtgtttt accggcaact cttcaagatt aaaacaggta
     9361 gcgcctttac tgacatgcac gcccgccgca gaataaaaag cgatatccgg gcaaaaatta
     9421 ttcagcgttt gctgaaaatc gatgggtttg aaaatggcgt tgctggcgtg aaattcacca
     9481 ccgcacagaa atgcgcggca atggggtttt tctttcagcg ccagaaaggt atttagcgaa
     9541 taacaaacgg cggtaaaagg gatttcatta tcaatcgctt caataatcca cggcgtggtg
     9601 gtgccacagt caaaaaagag ggtctgatcg ggttctacca gtgtcgcagc cagttttgcc
     9661 gcccggcgtt tttcttccac caggcgggat ttttgatcgc ttaacaggta atggctggcg
     9721 ctgcgcggtt ccagaacaat atagccgccg agcaaaacga cgggcgcact gtggttgttc
     9781 agatcgcgac gaatcgtcat ctccgaaacc ccaagcaggg cggcggcgtc tttaagatgt
     9841 aacttatcgc tgcgttttaa ttcttgcagc agctgcccga tacgctcttc gcgacgtgtt
     9901 tccataatcc ctctgaatag ttattgaagc gagccgctca atactacact ttttagcaga
     9961 gatcagtcac gcacccagcc tttgcggatc ggtaatgcaa aacagaagcg ataccacgat
    10021 tgcagacgtt gatagagttt atgccccatc gcttgccaga taatctgggc actaaggcag
    10081 ccaatcatac ctgccagcaa tccaccgagc atatccagcg gccagtggac gccaagataa
    10141 acgcgcgacc aggcaatgac gacggccagc accattaaaa gtgagccgga ccacaggcga
    10201 tgccagcata aaaatgccag tgcaaaggtg aaaatcaccg taccgtgatc gctcgggaat
    10261 gagtcatccg ccgcatgatg caggaagtta tagccgatat tttcgacaaa cggtcggtcg
    10321 tgcggaaaaa gatgtcccat cgtccaggac accaacaggc tgaccgccag cgcgatagcg
    10381 atttttatca ccagttgccg ttgtgctgta agcccccaca accaaagtac cgcggccagc
    10441 agcggcacca cggtaatcaa atctttagca ataaaaatcg ccaacgagat catccacgga
    10501 gccgagtctg gcgtcgcgtt aataagagag aatagagaga gattcaaatt ttccagcata
    10561 acttcccgac gcaaagtgat taaaagggag ccaatacagg caagtcgttg agaataaagt
    10621 gcaggttaaa ctgggtaaag cggcatcgtc ttatttccct caagcggcct gtttacggtg
    10681 ggtgattgta acgggcatag gttaaataaa acttaaagaa agcgtagcta tactcgcaat
    10741 aatgtaagaa tgtgcttaac cgtggtttca gctacaaaat tcgctttctc gttagctgcg
    10801 cttttattaa actctgcgcg attattattg gcgaagaaat tgcatgcaaa ataaattagc
    10861 ttccggtgcc aggcttggac gtcaggcgtt acttttccct ctctgtctgg tgctttacga
    10921 attttcaacc tatatcggca acgatatgat tcaacccggt atgttggccg tggtggaaca
    10981 atatcaggcg ggcattgatt gggttcctac ttcgatgacc gcgtatctgg cgggcgggat
    11041 gtttttacaa tggctcctgg ggccgttgtc ggatcgtatt ggtcgccgtc cggtgatgct
    11101 ggcgggtgtg gtgtggttta ttgtcacctg tctggcaata ttactggcgc aaaacattga
    11161 acaattcacc ctgttgcgct tcttgcaggg cataagcctc tgtttcattg gcgctgtggg
    11221 atacgccgca attcaggaat ccttcgaaga ggcggtttgt atcaagatca ccgcgctgat
    11281 ggcgaacgtg gcgctgattg ctccgctact tggtccactg gtgggcgcgg cgtggatcca
    11341 tgtgctgccc tgggaaggga tgtttgtctt gtttgccgca ttggcagcga tctccttttt
    11401 cggtctgcaa cgagccatgc ctgaaaccgc cacgcgtata ggcgagaaac tgtcgctgaa
    11461 agaactcggt cgtgactata agctggtgct gaagaacggt cgctttgtgg cgggggcgct
    11521 ggcgctggga ttcgttagcc tgccgttgct ggcgtggatc gctcagtcgc cgattatcat
    11581 cattaccggc gagcagttga gcagctatga atatggtttg ctgcaagtgc ctattttcgg
    11641 ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc tcgcgccgca ccgtacgttc
    11701 gctgattatt atgggcggct ggccgattat gattggtctg ttggtcgctg ctgcggcaac
    11761 ggttatctca tcgcacgcgt atttatggat gaccgccggg ttaagtattt atgctttcgg
    11821 tattggtctg gcgaatgcgg gactggtgcg attaaccctg tttgccagcg atatgagtaa
    11881 aggtacggtt tctgcggcga tgggaatgct gcaaatgctg atctttaccg tcggtattga
    11941 aatcagcaaa catgcctggc tgaacggggg caacggactg tttaatctct tcaaccttgt
    12001 caacggaatt ttgtggctgt cgctgatggt tatcttttta aaagataaac agatgggaaa
    12061 ttctcacgaa gggtaaaaaa atgcctgatt gctttgtgcg atcaggcatt ctcgaattaa
    12121 tggtgatggt cgtcaatctg gtgttcgata accatccctt cacctacgct ggcaagatgg
    12181 cgaacataag gatgcgggtg gtaagccgga gctggcgcag gagccacata gacggtttgc
    12241 ggcacagtcg cgacgctgac cgcttgtgga acgctgactg aatcaggaac cacaaccact
    12301 ttgtatccac tcggcacatc gacggtgaca ctttgcgcca tcgccacacc agtaaagccc
    12361 attaaaagcg cgcccaacag tagacaattt ttcataatca tttcgcctga aataatgcag
    12421 cgggttaatt tccgcgaatt atgcagcctt cattcaggta ttgtgttgcc gggcgtgctg
    12481 ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca catccagtac
    12541 gccttcacgg ttattggagc ctgcccggta ttttgccgct gcgacgaccg cgttgccggc
    12601 attttccatt gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt taccgccatc
    12661 gccaaagacc accacttcgc tgtcgtctat tccccataat ttctgcagtt ggcgaaggcc
    12721 attggcttta tgtacgccgg ggataatcag atcgatgctg ccgttgccgg tgtggaccgg
    12781 caccataata tcgccgatgg cctcatgtaa tgttttttgt acttgtggaa tcagttcatc
    12841 ggaaagattc aggccaaact taaagaagat atcttctaag ttgtcaaagt tatcgacgta
    12901 ttccagacgg tgataataca tttccgccac cattttcatg gcatcgtcat attttttgag
    12961 cgtataggcg ctattttttc cgcaggcaat aatttccact tccgggcgcg tcagcaaatg
    13021 ttccacgaca gtagtaaacg catccttcga cagctcgcca ttaaaaacat ctttgccttc
    13081 actcactacc cagccgccgt tttcagccac aaaggcaatt tcattagcaa tttcagggaa
    13141 gaaagagatc aactgataat attgattccc gctggcgacc acaaagcgaa tgccttgtgc
    13201 tttcatttgc tgatactgag ccataaaccg ctcacggtta taggtttttt gatcgcttaa
    13261 gaaagtacca tccatgtcta ccgcaattaa tttaatgctc atcaactatt ctccatcgcc
    13321 gtctgcgttt tggtatcggg tttggcgacg gctttcgcga caatagccgc gagaataacc
    13381 agcgccagta caaccagcat tgcactacgt aatccataat gttcgccgag atagcccagc
    13441 agcggcggcc cgacgaggaa agccagataa ccggtcgttg ctaccacact gacgcgggtt
    13501 ggtgcatcgg ggccggtatc gctggcggca gaaatggtca gcgggaagcc cagcgaggca
    13561 cccagtcccc agagtacaac agacacccca gcgacccagg cgctatcgac aaaaataatc
    13621 agcccaatac ccaacgcccc cattagcgca ctggcccgaa ccacggcaac gcgactgtaa
    13681 cggtcgatga accaaccgcc agtgaagcgt ccaacggtca tccccagggt aaaaccggca
    13741 taaatcagcg agccggaagt ggggctaaaa ccgtgaccgt caaccattaa taagggtaac
    13801 cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca ccacaacacc aatcagcagc
    13861 aactggatat cgcgataaaa aggtacgcct ttttcgccat gctgggtgcc atcggcagca
    13921 tttttgcccg taccgtcagg gattgcctga atggcgatat atataggcgc gatgcctacc
    13981 agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg
    14041 acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt
    14101 ttattcattt ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca
    14161 aaacttgccc caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt
    14221 gccagactta atatcatcat cccgaccaat gcgcaggaca tggtgaccag gatgacatta
    14281 cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc
    14341 gacagaccaa agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata
    14401 gcaggcgtac gggttgccca ggacgccatt aacaggcccg gcaaaaagaa gaacataaac
    14461 agcgcccagg ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc
    14521 aaaataagaa gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga
    14581 cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc
    14641 cacgctggag gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac
    14701 ccttgccggg gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt
    14761 gctggaggcg ttcagcagtt ttactgagat catgtcccgg caatatcagg cattttttag
    14821 cgatgttagt gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca
    14881 ggttgcaacg ccggataaca tggagctgat gtaccagctc tacgcgctgg ctagccgaaa
    14941 accgctatta aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca
    15001 atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca
    15061 ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt
    15121 tgcagggtag tagataaatt tcaggcaaca aaaaacccat caaccttgaa ccgaaatggc
    15181 ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac
    15241 tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc
    15301 agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga
    15361 cgttggcgat tgcgtaggtg cccgcatagc ccagcgccgg gatgttactg cgagctgtat
    15421 cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca
    15481 gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa
    15541 gactgacaat caatccggca atcaacatct gaccgccaat cgcgcccaga ccgttattaa
    15601 taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca
    15661 ccatgcttaa tgccccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc
    15721 ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc
    15781 tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg cagaatgcca
    15841 gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta
    15901 cgcggcgggc atcgccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat
    15961 caatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca
    16021 acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag
    16081 tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg
    16141 gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta
    16201 gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc
    16261 agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca
    16321 gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag
    16381 tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca
    16441 atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg
    16501 catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc ataccggaat
    16561 gacgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca
    16621 taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca
    16681 gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatcg cgaaaaaaaa
    16741 tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa
    16801 gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta
    16861 aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc
    16921 caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt
    16981 ttatattcac ggaggctaac ttcttgttta ctagtaaact gttgaaagaa atggtaattt
    17041 acgataatgt tttttaccag aattcagggc gcagattc
//
LOCUS       DAZFKY010000100        16768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFKY010000100 DAZFKY010000000
VERSION     DAZFKY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(272..697)
                     /gene="arsC"
                     /locus_tag="ACHKCL_002915"
     CDS             complement(272..697)
                     /gene="arsC"
                     /locus_tag="ACHKCL_002915"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5763843.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(710..1999)
                     /gene="arsB"
                     /locus_tag="ACHKCL_002916"
     CDS             complement(710..1999)
                     /gene="arsB"
                     /locus_tag="ACHKCL_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO5763844.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2053..2406)
                     /gene="arsR"
                     /locus_tag="ACHKCL_002917"
     CDS             complement(2053..2406)
                     /gene="arsR"
                     /locus_tag="ACHKCL_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO5763845.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3150..3233
                     /gene="dinQ"
                     /locus_tag="ACHKCL_002918"
     CDS             3150..3233
                     /gene="dinQ"
                     /locus_tag="ACHKCL_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5763846.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3287..4639)
                     /gene="gorA"
                     /locus_tag="ACHKCL_002919"
     CDS             complement(3287..4639)
                     /gene="gorA"
                     /locus_tag="ACHKCL_002919"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5763847.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4711..5553)
                     /gene="rlmJ"
                     /locus_tag="ACHKCL_002920"
     CDS             complement(4711..5553)
                     /gene="rlmJ"
                     /locus_tag="ACHKCL_002920"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5763848.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5756..7798
                     /gene="prlC"
                     /locus_tag="ACHKCL_002921"
     CDS             5756..7798
                     /gene="prlC"
                     /locus_tag="ACHKCL_002921"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5763849.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7806..8558
                     /gene="rsmJ"
                     /locus_tag="ACHKCL_002922"
     CDS             7806..8558
                     /gene="rsmJ"
                     /locus_tag="ACHKCL_002922"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5763850.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8607..10076)
                     /gene="dtpB"
                     /locus_tag="ACHKCL_002923"
     CDS             complement(8607..10076)
                     /gene="dtpB"
                     /locus_tag="ACHKCL_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5763851.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10242..10295
                     /locus_tag="ACHKCL_002924"
     CDS             10242..10295
                     /locus_tag="ACHKCL_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763852.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10273..10326
                     /locus_tag="ACHKCL_002925"
     CDS             10273..10326
                     /locus_tag="ACHKCL_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763853.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10393..10827)
                     /gene="uspA"
                     /locus_tag="ACHKCL_002926"
     CDS             complement(10393..10827)
                     /gene="uspA"
                     /locus_tag="ACHKCL_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5763854.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11218..11553
                     /gene="uspB"
                     /locus_tag="ACHKCL_002927"
     CDS             11218..11553
                     /gene="uspB"
                     /locus_tag="ACHKCL_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5763855.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11624..13123)
                     /gene="pitA"
                     /locus_tag="ACHKCL_002928"
     CDS             complement(11624..13123)
                     /gene="pitA"
                     /locus_tag="ACHKCL_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5763856.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13355..14557
                     /gene="yhiN"
                     /locus_tag="ACHKCL_002929"
     CDS             13355..14557
                     /gene="yhiN"
                     /locus_tag="ACHKCL_002929"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5763857.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14875..15927)
                     /gene="yhiM"
                     /locus_tag="ACHKCL_002930"
     CDS             complement(14875..15927)
                     /gene="yhiM"
                     /locus_tag="ACHKCL_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO5763858.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            16311..>16768
                     /locus_tag="ACHKCL_002931"
     CDS             16311..>16768
                     /locus_tag="ACHKCL_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763859.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDG"
BASE COUNT         4375 a         4185 c         4296 g         3912 t
ORIGIN      
        1 taatccagta cgtttttaaa ctgctgcact gtatcgctga tcactaagca gctcactgat
       61 acatcatgct ggacgcgata attggaatct cacgcaggga gtaatgtgta aatagcaaaa
      121 tcgcctgatg cgctacgcct gtcaggccta caaataacat acaatttatt gaattttcat
      181 gactttgtag atcggataag acgcgttagc gtcgcatccg acataaataa agcgcacttt
      241 tctaacaacc tgtggggggg atatagccgc tttatttcag gcgcttaccc gcttcatcaa
      301 ccactttctc gccatcttcc ttggtgaatg cgcctttttg cgcatctggc agaatttcca
      361 gcaccacttc tgaagggcgg cacaggcgag ttcccagcgt cgtcaccaca atcgggcgat
      421 taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt
      481 ccgcaaggcc cagctcctca tacggttcga cgtttttacg cagcagcgcg cgtacggaaa
      541 tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt tccagataat
      601 ggataatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc
      661 cgcaggccgg gttgtgataa atggtaatgt tgctcatatc agtatctcat tacaaagtga
      721 aagagagacg tagcgccagc gcagccagcg tcacaaacag cacaggcagg gtcatgataa
      781 tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa agtacgtgca
      841 gccagagcag cgttgccagg ctaccaatcg gggtgatttt cggccccaaa tcgcagccaa
      901 tcacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg ccatcaatgg
      961 acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg aaggcggtga
     1021 ggaatccggt gccgagcgtc gcggcccaca ggccgttatc cgccagcacg ttgagtacgc
     1081 cagaaagata ttccgttaat ccggcattgc gcaggccata aaccaccaga tacatgccga
     1141 gcgagaagat gacaatctgc cagggcgcac cgcgcagcac tttacccgta ttaatcgcat
     1201 gaccgcgttt agcgacgaca aataatatca acgcgcccac tgccgcaatg gcgctgaccg
     1261 gaatgccgag cggttccagg acgaaaaatc ccaccagcag aagcagtaaa acaacccagc
     1321 cagttttgaa cgtagcagga tctttaatcg cttctgcggg agatttcagc agcgccatgt
     1381 cgtagttctg cggaatatct ttgcggaaat agagatgcaa catcaccagc gtggcaacaa
     1441 tcgcggcgat atccaccggc accatcaccg aggcatattc gcgaaagccg aggccaaaga
     1501 aatctgcgga aacgatattc accaggttgg agacaataag cggcaggctg gcggtatcgg
     1561 caatgaatcc ggccgccatc acaaacgcca gcgtagtgcc tttactgaac cctaaagcca
     1621 gcagcatggc gatgacaatc ggtgtcaaaa taagcgccgc gccatcattg gcaaacaggg
     1681 cggcaacggc agcaccgagc aggacaatcc aggtaaacag caagcgaccg cgtccattgc
     1741 cccagcgtga aacgtgcagt gccgcccatt caaaaaagcc ggactcatcc agcagcaggc
     1801 tgatgataat aacggcaata aacgcagccg tcgcgttcca gacgatattc cacaccaccg
     1861 gaatatcgcc cggatgtact acgcccgtaa ctaacgccag tactgcgccg agcgttgcac
     1921 tccagccgat gcctaaacct ttcggctgcc agataaccaa tacaatggtc aggacaaaga
     1981 tagcgcctgc cagtaacata atgcctcccg gaaaaacaca tgtgcaaatc catatgtgtt
     2041 tagataaatt ttttaactac aagtgttctt actgtctacg gaacaatttt gtctggccag
     2101 gttgcggaca atcacctgaa ccttttcctg ttcgcatcgc caggcctgct caataatttt
     2161 cgcggcccat gatggaatat gcggtgataa gcggtaatga acccacttac cctgcttgcg
     2221 atccagcaac aaaccgcttt cacgaagaag cgccagatgg cgggagatct tgggctgcga
     2281 ctggtcgaga gcagtgcaga gatcacagac gcataactct cccagctcgc tgagcagtaa
     2341 aacgatgccc agacgggttt catcggcaag aattttgaac agttggatgg gtaacagaaa
     2401 tgacatattg cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg ataagtcaaa
     2461 aacatatatg gtttaacgaa tgtgtatgtg cagaggaagg taataggtgt gaattaagcg
     2521 ttggctattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac tttgcgtcgg
     2581 ttttttactt tccagcccag agttggtggc tctggctgga gtgagaatgc ccacatctaa
     2641 cggttcaata ctattagcca gtggctaaca ttgcaagcgt tagcgatttc ttcagctgga
     2701 gaagggacag tttgctatac atgttggtga aagaggattt atatactttc atcgggtcgc
     2761 tgccgtacca ggctttgtcc gaatccggat gtaaaaaaac cgacttcgcg tcggtttttt
     2821 tactcttcag ccctgagttg gtggctctgg ctggagtgag aatgccatca tctaacgcat
     2881 caatactaat accatactga aactattgca aggacatgct ggttttataa cctgaatata
     2941 ctgtatgatt atccagttag ctctgaggca ttttcactct ggcaatgcgc ataaacgctt
     3001 tcaaagtcct gttcagtggt acgggtggtg ccgttaactg atgctctggc cggagtgaga
     3061 ataccattat ctaacaatag ggcatgcgcc gtgacaggcg atggatgagt aagcggatgc
     3121 attctcactc catcgtatgg agaacacggg tgattgataa agcaatcatc gttcttgggg
     3181 cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac tgatagcgga
     3241 aacgtaatta agggcaaaga gcacaccact ctttgccctt taacatttaa cgcatggtca
     3301 cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt atcgaagtct tttttggttg
     3361 cgcccatctt cagtgccacc gcgaagccct gcaacatttc gtccatacca aaaccaatgc
     3421 cgtgaatacc gacaatcttc tcttctggcc caacgcacac cagcttcatg cggcacggct
     3481 ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac actttcacct
     3541 gatcgtcgcc gtactgctcg cgcgcctgcg gttccgttaa accaacagta ccaatcggcg
     3601 gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc ggcttgttat
     3661 taaacaggcg ttcagagaga cggcgacccg ctgcaactgc caccggtgtc agttctactg
     3721 cacccgtgtt atcgcccacc gcgtaaatac cttcaacgtt ggtgttttga tatttatcga
     3781 cgacgatata gcctttttcg ttggttttaa cgccagcggc ttcgaggttg atgttgtcat
     3841 tggcaggctc gcggccaatc gcccaaatca ggcaatccac cgtttcactg cgaccatctt
     3901 ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg atggcgttgg
     3961 tgtgcaactg cgggccttcg gcgttcatca cttcgaccag cgtttcagag atcatcgggt
     4021 cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg ccgaggccgt
     4081 taatcacgcc agccagctca acggcgatgt aacccgcgcc aacaaccgcc acacgctctg
     4141 gcaaagcagg aagggcaaag aaaccatcag aatcaatacc gtattccacg cccggaatat
     4201 ccgggtggct cggacgaccg ccggtggcga tcagaatatg atcggctgtg atggtttcac
     4261 cgttaacttc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt acatcaacgt
     4321 tatttttacc gagcacgttt tcataggaag tatgaatacg gtcgatatag gcggtacggc
     4381 tggcgatcaa cgtttcccag ttgaatttat tgatagtggt atcaaaacca taatccgggc
     4441 cgtacatatg gatcgcttca cggatttgcg ccgcgtgcca catcactttc ttcggtacgc
     4501 agccgacgtt cacgcaggtg ccgcccagct ctttggcttc aatcagcgcg catttctgac
     4561 cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg atggcgatgt
     4621 aatcatagtg tttagtcatg acagagtgtc cttatcgttg attaccgcga ttgtagcgcg
     4681 agacgtaata ggtgccagca atggctgcaa ttactctggc acgatccagc ttacggtggc
     4741 gtgcccggtg ccagctggaa ccagtttgct gtgcagccac ggcagcacgt tattcatctg
     4801 ctgttccagt ttccacggcg ggttaatcac aatcatgccg gaagcggtca tgccacggcg
     4861 atcgctgtct ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc
     4921 caggtcgtgg atcatgcgct taatttgctg gcgcagcacc accggatacc acaatgcgta
     4981 agtgccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgactaccg cctgatagtc
     5041 ggttttcatt tcatacggcg gatcgataag gattaaacca cggcgagaaa ccggcggcag
     5101 tttggcctta agctgctgga aaccgtcgga tttttcgacg cgcgcacggc tgtctttctg
     5161 aaattcagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc
     5221 ctgttcacgc agtaactgac gggcaatcaa cggtgaaccg gggtagtaac gcaactgacc
     5281 gctacggttg aagtgtttta ccacattgat gtacggctcc agctccgcag gcaaatcgtc
     5341 ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg catgttcgct
     5401 gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt
     5461 ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt
     5521 gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg taagtaaaaa
     5581 tcggcccgta ttcggggcgc acaaaagcct atcaggacag tataccgaaa agaggccgcc
     5641 gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat gctacaaaca
     5701 ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg cgctaatgac
     5761 gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc cggaacatgt
     5821 cgttccagcc gtgactaagg cattgaatga ctgccgcgaa aatgtggagc gcgtagtagc
     5881 gcaaggagca ccgtacacct gggaaaatct ctgccagccg ttggcggaag tggacgatgt
     5941 gttggggcgt attttctccc cggtcagcca cctgaactcg gtgaaaaata gcccggaatt
     6001 gcgcgaagcg tacgaacaaa ctctgccgct gctgtcggaa tacagcacct gggtagggca
     6061 acatgaaggg ctgtataaag cgtaccgcga cctgcgcgat ggcgatcatt acgccacgct
     6121 gaacacggct cagaaaaaag cggttgataa cgcactgcgc gattttgaac tctcaggcat
     6181 cggtctgccg aaagagaaac aacagcgtta cggcgaaatt gccactcgtc tttctgaact
     6241 gggcaaccag tacagcaaca acgtccttga tgcgacgatg ggctggacta aactcgttac
     6301 cgacgaagcg gaactggcgg ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc
     6361 cgaagcgaaa gagctggaag gctacctgtt gacgctggat atcccaagct acttgccggt
     6421 aatgacctac tgcgacaacc aggtcttgcg tgaagagatg tatcgtgctt acagcacccg
     6481 cgcttcagat caaggtccaa acgccggtaa atgggataac agtaaggtga tggaagagat
     6541 cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg cgtttaaatc
     6601 actggctacc aaaatggcgg aaaacccgca gcaggtgctt gatttcttaa ccgatctggc
     6661 aaaacgcgcg cgtccgcagg gcgaaaaaga gctggcgcaa ctgcgtgcct tcgccaaagc
     6721 cgaatttggc gtggatgagt tgcaaccgtg ggatatcgca tactacagcg aaaaacaaaa
     6781 acagcacctc tacagcatca gcgacgaaca actgcgtccg tacttcccgg aaaacaaagc
     6841 ggttaacggc ttgtttgaag tggtaaaacg tatttacggc atcaccgcta aagagcgtaa
     6901 agatgttgat gtctggcacc cggatgtacg tttcttcgaa ctgtatgacg agaacaacga
     6961 actgcgcggc agtttctacc tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg
     7021 gatggatgac tgcgtaggcc agatgcgtaa agccgatggt tcgctgcaaa aaccggtcgc
     7081 gtatctgacc tgtaacttca accgcccggt gaatggtaaa ccggcgctgt tcactcacga
     7141 cgaagtgatc actctgttcc acgagttcgg tcacggcctg caccatatgc tgacccgcat
     7201 cgaaaccgcc ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg aactgccgag
     7261 tcagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct ctggtcacta
     7321 cgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg cgaagaacta
     7381 ccaggcggcg ctgtttattc tgcgccagct ggagttcggc ctgttcgatt tccgcctcca
     7441 tgccgagttc cgcccggatc agggagcgaa aatcctcgaa actctggcag aaatcaagaa
     7501 actggttgcc gtagtaccgt ctccatcctg gggccgtttc ccgcacgctt tcagccatat
     7561 tttcgccggt ggttatgccg caggttacta cagctacctg tgggctgacg tactggcggc
     7621 agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt
     7681 cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt tcaaacgctt
     7741 ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta agggctgatc
     7801 attcagtgaa aatctgctta attgatgaaa caggcaccgg agacggtgcc ttatctgttc
     7861 tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg gtgttaacgc
     7921 cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcatc tttgttgatt
     7981 ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcggt gaggcggtgg
     8041 cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgct accgcagggc
     8101 tggggcgcga tgcctttgtg ctggcttcgg tcggctgccg cgtgcggatg ctggagcgta
     8161 atccagtcgt tgccgcgcta ctcgacgacg gcctggcgcg tggatatgcg gatgcggaaa
     8221 tcggcggctg gttgcaggag cgtttgcagt taattcacgc ctccagcctg acggcgctga
     8281 ccgatattac cccgcgtccg caggtggtct atctcgaccc gatgttcccg cataagcaga
     8341 aaagcgcgct ggtgaaaaaa gagatgcgcg tctttcagtc gctggtggga ccggatcttg
     8401 atgccgatgg attactggag cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc
     8461 gcccggacta cgcgccaccg ctagcgaatg tcgccacgcc aaacgcggtt gtgactaaag
     8521 ggcatcgctt tgatatctat gcaggtacgc cagtgtaaaa gtaaagcagg gtagcgataa
     8581 cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat catgcgtttc
     8641 agccacggca ccatcaacag catcactact gcaacgccca gcgtgaccag accaatctta
     8701 ccaaacacgt tggtatagac aggcaacgtc tcaagcggat cggtaatgtt gtctggtact
     8761 gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgtgt caggaaccac
     8821 atcccgagaa taaagcccat caaatgctgc ggcaccagag cagcaatcat cgccaggcca
     8881 agggcgctaa taaacagttc acctaagctc tggaataagt acaccagcac gataaaccat
     8941 ggcgatgtca gcccttgtgc atccgcaaac cacattcccg cagctgccgc cgtcaaaaag
     9001 cccagtgagc acataaacat gccgagagta aatttcatcg gcatcgagag gtctttgcct
     9061 ttgttaccca gatgcgtgta aatgcctgcc agtattgggc tggcgagtac cacccagaac
     9121 gggttaagcg cctggaagct gaccgggttg atggaaaaac cgagaatttc atgatgcacg
     9181 ttgttgatgg caaagaagtt cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc
     9241 accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt tttatccagc
     9301 ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat cagcaccaga
     9361 ttggcgactt ctacgttgtg catcagccag gcgcagacga agatcatcac cacgctgcca
     9421 agtaatacgt acaacagttt gctgaagctc attggccgga agtcgggctc agaaccaatg
     9481 tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa ccccgcaccg
     9541 cacaggttgt aggtgactga atagccgaat ttatcagcga tcacaggggc cagcgataac
     9601 gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgtgcc atcaaggcgc
     9661 ggatctttcg gcggatagca cttcgaaagc aagctggctg ggttagcttt aaacaggccg
     9721 ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag tagcgacatg
     9781 ccggtcatga agtagccaat cgccagcaca agtgctccaa gaacgatggt gcgtttggtc
     9841 cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac cagtgcagca
     9901 aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa
     9961 accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag
    10021 atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attcatattt
    10081 attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag
    10141 tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac actattgcaa
    10201 cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta aatattgtat
    10261 tggctgggga ttttgattgc gaacgcagac cgtaggccag ataaggcgtt tacgctgatc
    10321 aggtaacaag gcgcgacaaa aagcaaaacg cccgcatatg cgggcgttca ggaacacgtc
    10381 gtggagggaa gattattctt cttcgtcgcg cagcggaaca atcagcatat caacgtgaac
    10441 ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct ggtggtgacc
    10501 acaaaccact agatccatat cgtatttctt gattgcatcg accagaacct ggcccaggtc
    10561 gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttagtggaaa gctcggtcag
    10621 cgcatgatgt gtctcttcag agatgcgttt ctgcatatca ccaagattca catcaataag
    10681 cccggtatat aggtcagagt agtttacatc tacgtggatc agagaaactt tcgcattgta
    10741 tgggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg agagatcgac
    10801 cgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt gtcgatgact
    10861 ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg
    10921 gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc gacccggatg
    10981 atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg aatatattga
    11041 tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta tagagccgct
    11101 cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg attggcagcg
    11161 tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg aggaggtatg
    11221 ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc
    11281 tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc attgctctat
    11341 caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa acaggtgcgt
    11401 ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga gtttattcgc
    11461 cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg tctggtggtg
    11521 gtcagcctga ttgccttgat gatttggcat taagcacttc agcaataata acgcataaaa
    11581 aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta ctattacagg aactgcaagg
    11641 agagccagta aagcccgccg gaaagcagta ccgcagccgg aagggtaaac acccaggcca
    11701 tcagaatgct ggtcacggtt ttacgctgta agccgccacc atctaccacc atcgtccccg
    11761 cgacagaaga ggagagtacg tgagtagtgg aaaccggcat cccggtataa ctcgccaggc
    11821 cgatagacac tgccgcagtc atctgggcag acatcccctg agcgtaggtc atgcctttct
    11881 taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc gtaccgatac
    11941 ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc atactcgatg gtgctaagca
    12001 tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacgcca ggcatcttca
    12061 ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat
    12121 caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc aggcggttga
    12181 gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt gcgccagctt
    12241 ccggagccgg tactaactga tcagcaccag tagcttgttt cagcagcgca ggatgctgct
    12301 caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg tagccagtgg
    12361 cattcatgtt caccacgaag cctgctggtg cgacaccaat caataccaac ataaccagac
    12421 caatgccttt ctgaccatcg tttgcgccgt gcgaaaacgc cacgccgata gcggaaagga
    12481 tcagcgcgat acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg
    12541 ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga
    12601 aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa
    12661 tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat
    12721 tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggcaaac
    12781 caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg
    12841 caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg
    12901 cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc gctgccataa
    12961 ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg
    13021 tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata
    13081 acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt
    13141 tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata
    13201 gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat
    13261 tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac
    13321 aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat
    13381 aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggcag gacgccgggt
    13441 tctgctgatc gataatggta aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg
    13501 ctgcaacttt accaaccttt atgtcgaacc gggcgcttat ctgagccaga atccgcattt
    13561 ttgtaagtct gcactcgcgc gttttaccca gtgggatttc attgatctgg tcaataaaca
    13621 cggcatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact ccgcgcagca
    13681 gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtgaccgtca gattgcgtag
    13741 cgaagtgctg agtgtggcga aggatgatac aggcttcacg cttgaactga acggcatgac
    13801 tgtcggttgt gaaaagctgg tcatcgcgac cggcgggctg tcaatgccgg gcctgggcgc
    13861 gtcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc
    13921 gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg
    13981 cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg agaacttact
    14041 cttcacccat cgcggcttgt ctggaccggc ggtgttgcaa atttccagct actggcaacc
    14101 gggggaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct tcctgaatga
    14161 gcagcgtaac gcacatccga atcagagcct gaaaaacaca ctggcggttc atctaccgaa
    14221 gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct
    14281 caacgtgcgt gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa
    14341 cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ccaacgaact
    14401 ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg tactttatcg gcgaagtgat
    14461 ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc
    14521 ttgtgcgcag gatttgattg cagcgaagtc gtgctgagct atttttccta aagatgaaaa
    14581 ttattagagt cgcattaaaa atgagctaat tttgatagtg gctatcttgt gattattttc
    14641 taatgagccc gtgaactgaa ccccccatgc ttaatataag gtggagggaa aggtgattga
    14701 aaactcaccc agtgggctga tctttataaa atatgaacag gttgaaaaaa aaacagtaac
    14761 tttgctgttt tttatactta accactatgc attaatgctg cgttatttca ttctacctaa
    14821 gaaaaaccag cgttacgcaa atggttaacg ctggttttat ccggtacgtt gcaattattt
    14881 tttagcagaa cccgcttcta atattgaaac gattgagaac aacgtaaagc ataccgctcc
    14941 caaaccgacc agaacccgcg aaggaataaa atatcccata tcggtctgcg ccatttccgc
    15001 aagaaacgat gctaggaaaa ggcaaaacag gcaggtgaag acgggaatca tcggtattct
    15061 gttggctaac gaacaggtac gtctccagac cagtgccagt agccagactt ttgagaatat
    15121 gctgtaacag atcattccca agcaaatgag gataataccg ggagccagtc gggcgcttgc
    15181 atcagaactg actaagacgt atatgccctg cagcaccgtg attgagccga gaaaaataac
    15241 ccaataacac cacagccagt gctctttcgt tgaaaacgta ttacgtgttt gatggacgat
    15301 tgtggcaaca aggccaatta gacaggcaca gattgcggtt aacccgagca atacgtgacc
    15361 ggcgacataa tgcggagtta tgtcggcact acgtaacagc gtaatagacc aaatgaaacc
    15421 gagcagggtg agtaacacgg gaacggcaat gaggcaatta ccgattaatg aagaataagc
    15481 ctgtaccggt gttccgtcgc tcttgctccc cgctgcgttt ttaggaatga gcagaaagtg
    15541 accggatgac gctgccaccg tcgatacaca ggcggcaatc ataccaacgc cgaaaataac
    15601 atggccggcg acaaactcgt ctgccatcac atcattacct gctaacagtg cccaacccca
    15661 tatcatggtg ataattgacc ccgcatagcc aataatgggg aacaatgtat tgtaaaacgt
    15721 attaacgccg cgcgtgagct gcgaaatgat aataaatgcg gtagtgaata atgccaggca
    15781 tatggctgcc agagaaatga gcacatgacc cgcgacgaaa tactcgcttt gccctgaact
    15841 ttccagcaca aagccaccta acgcgataca aatcaagccc ataattaaag ggattaattt
    15901 gaaaagccac ccgatatata tgttcacttt aaactcctca ggtatccctg ctaatattga
    15961 aatgtgcatt aatgataatt tcagcctgtt aatacaatga cgagatatca ttaaagacaa
    16021 atgcatagca taactatagc ggggtttact ttgcagagcg ctacttttca gcgttcaaaa
    16081 ggccatgata aataaatatt tatcctaaga ttaattctta ttctgttgtt ttttacaatt
    16141 atcttccagg gagggattct ttttcattat ttggtaaaag accggatggt ctcggtgctt
    16201 aaaatcagcg agtatatacg catcattgct ttatatcgtc aaaaaatatg tctttcagac
    16261 atgtatcatt tattttgtta ctaacaagtc aaaaatatca cgggttattt atgaaagtat
    16321 ttcacaatat atttaagtac atctcttcaa accatcaaga taagcattca gataaagtta
    16381 ataatcatca acaccatggc aaggttgata aaaaacatcg ttcagagata gttgaaattg
    16441 ataagctgga taaacattcg caaatagata acgattttgg cctgcatatc atctatttcc
    16501 tgcaacatgg ctgctggaac gtaactgaac atagtcctca gatagagaaa atatggtttt
    16561 acaatagtga gccatctata gatattaaag aatataatag tttcgccgac aatactactg
    16621 acacgtttat ctttacaatt ataccagaca ataaccatgt gataaaatta tcgtctccca
    16681 ttaccgttac gattgagcgt aaggacggtt attattttat taatttctcc ggggataaat
    16741 cagacataat ttataaagtt gatgggtt
//
LOCUS       DAZFKY010000101        16753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFKY010000101 DAZFKY010000000
VERSION     DAZFKY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(146..1429)
                     /gene="yeaH"
                     /locus_tag="ACHKCL_002932"
     CDS             complement(146..1429)
                     /gene="yeaH"
                     /locus_tag="ACHKCL_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5763860.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1542..3476)
                     /gene="yeaG"
                     /locus_tag="ACHKCL_002933"
     CDS             complement(1542..3476)
                     /gene="yeaG"
                     /locus_tag="ACHKCL_002933"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5763861.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3912..4658
                     /gene="mipA"
                     /locus_tag="ACHKCL_002934"
     CDS             3912..4658
                     /gene="mipA"
                     /locus_tag="ACHKCL_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5763862.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4748..5602
                     /gene="yeaE"
                     /locus_tag="ACHKCL_002935"
     CDS             4748..5602
                     /gene="yeaE"
                     /locus_tag="ACHKCL_002935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5763863.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5653..6537)
                     /gene="yeaD"
                     /locus_tag="ACHKCL_002936"
     CDS             complement(5653..6537)
                     /gene="yeaD"
                     /locus_tag="ACHKCL_002936"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5763864.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6621..7616)
                     /gene="gapA"
                     /locus_tag="ACHKCL_002937"
     CDS             complement(6621..7616)
                     /gene="gapA"
                     /locus_tag="ACHKCL_002937"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5763865.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7958..8371
                     /gene="msrB"
                     /locus_tag="ACHKCL_002938"
     CDS             7958..8371
                     /gene="msrB"
                     /locus_tag="ACHKCL_002938"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5763866.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8413..8685
                     /gene="yeaC"
                     /locus_tag="ACHKCL_002939"
     CDS             8413..8685
                     /gene="yeaC"
                     /locus_tag="ACHKCL_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5763867.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9055..10131
                     /gene="ydjL"
                     /locus_tag="ACHKCL_002940"
     CDS             9055..10131
                     /gene="ydjL"
                     /locus_tag="ACHKCL_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5763868.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10158..11537
                     /gene="ydjK"
                     /locus_tag="ACHKCL_002941"
     CDS             10158..11537
                     /gene="ydjK"
                     /locus_tag="ACHKCL_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5763869.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11554..12597
                     /gene="ydjJ"
                     /locus_tag="ACHKCL_002942"
     CDS             11554..12597
                     /gene="ydjJ"
                     /locus_tag="ACHKCL_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO5763870.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12618..13454
                     /gene="ydjI"
                     /locus_tag="ACHKCL_002943"
     CDS             12618..13454
                     /gene="ydjI"
                     /locus_tag="ACHKCL_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5763871.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13459..14391
                     /gene="ydjH"
                     /locus_tag="ACHKCL_002944"
     CDS             13459..14391
                     /gene="ydjH"
                     /locus_tag="ACHKCL_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5763872.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHCTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14401..15381
                     /gene="ydjG"
                     /locus_tag="ACHKCL_002945"
     CDS             14401..15381
                     /gene="ydjG"
                     /locus_tag="ACHKCL_002945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO5763873.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15518..16276
                     /gene="ydjF"
                     /locus_tag="ACHKCL_002946"
     CDS             15518..16276
                     /gene="ydjF"
                     /locus_tag="ACHKCL_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5763874.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16394..16495
                     /locus_tag="ACHKCL_002947"
                     /pseudo
     CDS             16394..16495
                     /locus_tag="ACHKCL_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16539..>16753)
                     /locus_tag="ACHKCL_002948"
     CDS             complement(16539..>16753)
                     /locus_tag="ACHKCL_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO5763875.1"
                     /translation="NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL
                     GRLGHIPPAEAEKAYYASIRNDDLAA"
BASE COUNT         4329 a         3782 c         4222 g         4420 t
ORIGIN      
        1 tttccgaatg attgttttct ggaactaaat tgtgtaaagg ttatgctgtc atatttaaag
       61 cgattgtaag ctaatgtatg taataaatga gataacttac agtgagctaa aaatcagcca
      121 ggcgataatg acccggctga tagttttagt ctttagctgt tgcattttgc ttatgaaaca
      181 gttcacggaa caccggataa atatcatcct ggtcgcggat atgctgcatc gcaaagttgt
      241 cgaaagtaga ttgcagatgc tcatattctc gccacagtgt ctgatgcgcg cgacgggtaa
      301 tttcgatata gctgtaataa cgaacaacgg gtaataattt tttcgccaga atttcatggc
      361 aaagcggaga gtcatcggcc cagttatcgc cgtccgatgc ttgtgcagcg taaatattcc
      421 actgtgccgg gttataacgc tctttcacta cctcatccat cagtttcagg gcgctggaaa
      481 caatggtgcc gccagtttcc tgcgagtaga aaaactcatg ttcatcgact tctttcgcct
      541 gggtatgatg gcggatgtat acgacttcca cgttcttata cgttctgctg aggaacagat
      601 acagcagaat ataaaaacgc ttagccatat ctttagtgga ttgatccatt gaaccggaaa
      661 cgtccatcag gcaaaacatc actgcctggc tggagggatc gggccgcttc tcgtagttct
      721 tgtaacgtaa atcgaaggtg tcaataaaag ggacgcgttc aattttggca cgtaattctg
      781 caatttcttt gcgcagacgt tcctcttcca gcagctgcgc aggttcactg ttgctgatga
      841 tggccaaatt ctcttccagt gcatgaagtt cccgccgctt gcctgccgtc atggctgtgc
      901 gtcgcgccag tgagttctgc aaggaacgca caacgctgat attggccgga acgccgttag
      961 cggtataacc cgcccgatgc gttttatatt cggtcagctg gcgttgttgg ttttgtttca
     1021 gattcggtaa ggccaaatct tcaaagagca gatcaagata ctcatctttc gaaatctgaa
     1081 agacaaattc atcctgacct tcaccatcct ggctggcctg gccctgacca ctgccggaac
     1141 ctccgccgcc accctgggga cgttcaattc ggtcgttctg aacgaaatgg tcattgcccg
     1201 gatgcacgcg gtggcgcaga ccgccacgcc cctgatgaaa cattggttcg ctaatatctt
     1261 ccgtgggaat ggatacggac tcgccgctgt cgacgtcagt caccgaacgc ttattaatgg
     1321 cctcggagat cgactgttta atttgcgctt tataacggcg taaaaaacgc tggcgattca
     1381 ccatgctttt gtttttgccg ttcagacgcc ggtcaataaa ccaggtcata tgccccccgt
     1441 actacatttg ccaactgtat gctacgcctg tgttgccatt tctctggccc acggttcgct
     1501 gtcgttgtag gcccggtagc atgtgtaccg ggccagggtt gttaagacga tttacgtacg
     1561 cgcaaatacc attcgcacag taaacgcacc tgtttacggg tgtagccttt ttccatcata
     1621 cggtcgacaa agtcgtcgtg tttcttctgc tcgtcggttg acgttttggc gttaaacgag
     1681 ataaccggca acagctcctc ggtattggag aacattttct tctcgataac cgtgcgcagt
     1741 ttttcatagc tggtccagtt cggattgcgt ccgctgttat tggctctggc gcgcagtacg
     1801 aagttaacaa tctcgttgcg gaaatctttt ggattactga tccccgccgg tttctcgatt
     1861 ttctccagct cggcgttaag agactcgcgg tcaaacagct gcccggtatc cggatcgcga
     1921 tactcctgat cctgaatcca gaaatccgcg taggtaacat aacggtcgaa aatgttttgc
     1981 ccatattcgg aataggattc aaggtaggcc gtctggatct ctttgccaat aaactcggca
     2041 tatttcggga tcagataacc tttcaggaac tccagatagc gttctgcctg ctcttgtggg
     2101 aactgctcgc gctcgatctg ctgttccagg acgtagaaca gatggaccgg gtttgccgcc
     2161 acttctacat gatcgaagtt gaacacgcgg gagaggatct taaacgcaaa acgcgtcgac
     2221 agaccgttca taccttcatc gacaccggcg tagtcacgat attcctgata cgacttggct
     2281 ttgggatcgg tgtctttcag actttcgcca tcataaaccc gcatctttga ataaatgctg
     2341 gagttttctg gctctttcag gcgcgaaaga atggaaaaac gtgacagtgt ttcgagagtg
     2401 ccaggggcgc atggggcgtg agtcaattca ctgtgattaa gcaatttctc gtagattttg
     2461 atctcttcgg aaatgcgcaa gcaatacggc accttcacga tgtaaacacg atcgaggaag
     2521 gcttcgttgt ttttgttatt acggaaagtg acccattcgg actcgttcga gtgggcaaga
     2581 ataatcccgt tgaacggcag ggcggagata ccttccgtcc cgttgtagtt accttcctga
     2641 gtggcggtta acaagggatg cagcactttt atcggtgctt taaacatctc gacaaattcc
     2701 atgatccctt ggttggcgcg gcacagcgca ccggaatagc cgtaggcgtc cgggtcattc
     2761 tgcgcgtaat gttcgagttt acgaatatcg actttcccaa ccagcgcgga gatgtcctgg
     2821 ttgttctcat caccgggttc cgttttggcg atagcaattt gttgcagaat tgacggccag
     2881 accttcacta cccggaactt agtgatatcg ccaccaaatt catgcaggcg ttttgccgcc
     2941 cacggcgaca tgatggtgcc gagataacgg cgaggaatgc catactcttt ttccagaatc
     3001 tgcgcatctt cctgcggatt gaaaagacag aacggatgat cgttgaccgg gctacgctca
     3061 ccgttggcgc tcaatacata aatcggtacg agctgcatta atgatttcag tcgctcagca
     3121 agcgatgatt tacccccacc cacaggcccc agcagataca ggatttgttt cttctcttcc
     3181 agcccctgag ccgcgtgttt cagataagag acaatctgtt caatcgcgtc ttccatgccg
     3241 taaaactctt caaacgccgg ataacgtgca atgacccggt tagaaaagag tcgagaaagt
     3301 ctgggttcct gggctgtatc gaccatgaca ggctcaccga tagccatcaa tagccgctca
     3361 gccgcgttgg cataagcact gcgatcttgc cgacaagtgg taagaaactc ctgcagtgtg
     3421 aactcttcgt ccttggcagc ttcatatcgc tggcgatagt gatcgaatat attcatggta
     3481 tgccgtcctt tcgtttttta gcacaggtta agagccgttc atatgaatag tagaggctcc
     3541 cggaagaggt aaactgaacg acgtctcttg ctaaaacaca gcaactctca tgccatttta
     3601 tcgacgatga gaacaacgtg cccggtgata caccttcaaa attaagcgta gatggcaaat
     3661 ggaaaacttg cctacacggc tttcttaatt tcaatggcat atcaacaact catccataaa
     3721 ttttgcataa ttaatgtaaa gaccaggctc gccagtaacg ctaaattcat ttggctgtaa
     3781 gcgcggtgtc atccgcgtca ggaaaattaa acagttactt taaaaaatga aaacgtaaaa
     3841 aggttgggtt tcgatgtatt gacgggtaaa ctttgtcgcc cgctaaacat ttgtttaagg
     3901 aatgattaat tgtgaccaaa ctcaaacttc tggcacttgg agtgcttatc gcaacgtctg
     3961 caggcgtagc gcacgctgaa ggtaaatttt ccctgggcgc aggcgtaggt gtcgttgaac
     4021 acccatataa agattacgat accgatgttt acccagtacc ggtaatcaac tatgaaggcg
     4081 ataacttctg gttccgtggc ttaggtggtg gttactacct gtggaatgac gcaacggata
     4141 aactttcaat taccgcttac tggtcgccgc tttacttcaa agctaaagac agtggcgatc
     4201 accaaatgcg tcacctggat gaccgtaaga gcaccatgat ggctggtctg tcttatgctc
     4261 actttaccca gtacggttac ctgcgtacca ccctggctgg cgataccctg gataacagca
     4321 acggcatcgt ctgggatatg gcctggttgt atcgttacac caacggtggc ctgaccgtga
     4381 ctccgggtat tggtgtgcag tggaacagcg aaaaccagaa cgaatactat tatggcgtat
     4441 cgcgcaaaga gtccgctcgc agcggtctgc gtggctataa ctcgaacgac agctggagcc
     4501 cttacctgga gctgagcgcc agctacaact tcctcggcga ctggagtgtt tacggtaccg
     4561 cgcgctacac ccgtctgtct gatgaagtta ctgacagccc gattgtggat aaatcctgga
     4621 ctggcctgat ttctaccggg atcacctaca aattctgata atgcatcttt ccagggcatt
     4681 ttgtgcataa aaacagggcg tcacgccctg ttttgcatta cggtggtgca atacctggga
     4741 ggaaacaatg caacaaaaaa tgattcaatt tagtggcgat gtctcactgc cagccatagg
     4801 gcagggaaca tggtatatgg gcgaagatgc cagtcagcgc aaaacagaag ttgctgcact
     4861 acgcgcgggc attgaactcg gtttaaccct cattgatacc gccgaaatgt atgccgatgg
     4921 cggtgccgaa aaggtggttg gggaagcatt aaccggtctg cgagagaagg tctttctcgt
     4981 ctctaaagtc tatccgtgga atgctggcgg gcaaaaagcg ataaatgcat gcgaagccag
     5041 tttacgccgt ctcaatactg attatctcga tctctactta ttacactggt ctggcagttt
     5101 cgcttttgaa gagactgttg cagtgatgga aaaattgatc gcccagggaa aaatccgccg
     5161 ctggggcgtt tcgaaccttg attatgctga tatgcaggaa ctctggcagc tgccgggggg
     5221 aaatcagtgt gccactaatc aggtgcttta ccatctcggt tcacgaggga ttgagtacga
     5281 tctactcccc tggtgccagc aacagcagat gccggtgatg gcttacagtc cgttagccca
     5341 ggccgggcgg ttgcgcaatg gactgttaaa aaacgcggta gtcaacgaaa ttgcacatgc
     5401 tcacaatatc agcgcggcgc aagtattgtt ggcgtgggta atcagccatc agggtgtgat
     5461 ggcgattcca aaagcggcca cgattgccca tgtccaacag aatgcggctg tgcttgaggt
     5521 cgaactttcg tcagcggaat tagccatgct ggacaaagcg tatccggcac caaaaggaaa
     5581 aactgcgctg gatatggtgt gatgttgata cgtgccggat gcatcgcacc cggcacacaa
     5641 cattgacgca aattaacgtt tggcaacgcg aatggattgc gccagatgtg caggtttttc
     5701 tttggtcact ttttgcattt ctgaggcgta agctgtttct acacaaacaa aggtcttgta
     5761 gccatcgtcc ggcatgtcgc ccatgctaac tgaaagcgcc ggtcccgggt tccagcctac
     5821 aacgttcaga tgatgctggt ggcctacggc gataatacga ttcagcgctt catcattaat
     5881 cacgctgcaa tcttgcggat tcagatacac gcgatcggta cggtcaggga aggtctgaat
     5941 accgtcggtc agtacatctt ctttcgcgtc atttacttta tcaatgaagc gatcgcccag
     6001 cccactgacg cttaccttag cgatatcacc cacgttaaag taggtatgca gggcagaggt
     6061 ggtttcaaat tcgccgtgtg cttcaagatc gatttcacag tttttatcca cgcggaaatg
     6121 cgctaacaga gtaaagtcgt gcagccagaa ttttttcgtc tcttcgcttt gtgtcaattc
     6181 aaaagtcagc gctacgccat tagcatcttc acgatgtgat ttcagcgtcc acggcaggtt
     6241 gcgggcaaaa ccgtgcgcag gcaggccttg ttgtgccgcc ggaccaaacc acggccagca
     6301 aaccggtacg ccaccgcgaa tagcaacgcc atttttgaac ggtgtgttgt tgctcaacca
     6361 cagaacttct tcttcacctg caggtttcca cgagagaagg tgtgcgccct gtaatgcaaa
     6421 agaggctttt acctggggat gatcgactac aatgaggtcc agttcatcca gtttacgacg
     6481 ggagaggaca ggggagattt gttcgatgac cggaagggca aaaattttct taatcatgac
     6541 gcagtccttt aacttcattt tatcaggtaa aaaaaagagc gaccgaagtc gctcttttta
     6601 gatcacagtg tcatctcaac ttatttagag atgtgagcga tcaggtccag aactttgttg
     6661 gagtaaccgg tttcgttgtc gtaccaggat accagtttca cgaagttgtc gttcagagcg
     6721 ataccagctt tagcatcgaa cacggaagtg caaacttcgc cgttgaaatc ggtagatact
     6781 acgtcatctt cggtgtagcc cagaacgcct ttcatttcgc cttcagcagc agctttaacg
     6841 gcagctttga tctgctcgta agttgcagct ttttccagac gaacggtcag gtcaactacg
     6901 gatacgttcg gggtcggaac gcggaacgcc ataccagtca gtttgccatt cagttctggc
     6961 agtactttac ctacagcttt agcagcaccg gtagaggacg ggatgatgtt ctgggaagcg
     7021 ccgcggccgc cgcgccagtc tttgtgagac gggccatcaa cggttttctg agtagcggta
     7081 gtagcgtgaa cggtggtcat cagaccttcg atgatgccga agttatcgtt gataacttta
     7141 gccagcggag ccaggcagtt ggtggtgcag gaagcgttgg aaacgatgtc ctggccagca
     7201 tatttgtcga agttagcgcc tttaacgaac atcggagtgt tgtctttaga cggaccagtc
     7261 ataaccactt tcttcgcacc agcggtgatg tgtttacgag cagtttcgtc agtcaggaac
     7321 agaccagttg cttcagcgac aacgtcaaca ccaacttcgt cccatttcag gttagccgga
     7381 tcacgttcag cggtaacacg gattttttta ccgttaacga tcagatgacc gtctttcact
     7441 tcaacggtac cgtcgaaacg gccgtgagtg gagtcatatt tcagcatgta tgccatgtaa
     7501 tcagcgtcta acaggtcgtt gattgcaacg atctcgatgt cagaacgttt ctgagcagca
     7561 cggaaaacaa tgcgaccgat acggccaaaa ccgttgatac ctactttgat agtcatatat
     7621 tccaccagct atttgttagt gaataaaagg ttgcctgtaa aattacaaaa accttacgca
     7681 gcgtcaagcg gaatcgtgtc aatcattgcg acaaatcaat cctgtgccta agcattacgc
     7741 gactgactcg cctcactctt cctttgggct tgagaccaca tggggtcggc gccccgaatt
     7801 ttaaagggca attacgataa aaatgtgatt ttcatcacga tttaggtgca gccatttcgt
     7861 cgagatcaag tttaggacaa aattttgcca gttggtgtta atgttttgtt agaatcagtc
     7921 acgtaatgtg agcacgatta aagtgagatg tgagcaaatg gctaataaac cttcggcaga
     7981 agaactgaaa aaaaatttgt ccgagatgca gttttacgtg acgcagaatc atgggacaga
     8041 gccgccattt acgggtcgtt tactgcataa caagcgtgac ggcgtatatc actgtttgat
     8101 ctgcgatgcc ccgctgtttc attcccaaac caagtatgat tccggctgtg gctggcccag
     8161 tttctacgaa ccggtgagtg aagaatccat tcgttatatc aaagacttgt cacatggaat
     8221 gcagcgcata gaaattcgtt gcggtaactg tgatgcccat ctggggcatg tcttccctga
     8281 cgggccgcag ccaacgggcg aacgttattg tgttaactct gcttctttac gcttcactga
     8341 tggtgaaaac ggcgaagaaa tcaacggttg aaaaaacgat tcagcaactt attccacagg
     8401 agcggaaaaa ttatgaatct tgatgacatt atcaacagca tgacgcctga ggtataccag
     8461 cgtttgtcga ccgccgttga actggggaaa tggcctgatg gcgttgcgtt gaccgaggaa
     8521 caaaaagaga actgcctgca actggtgatg ctgtggcaag cccgcaataa taccgaagca
     8581 cagcatatga caattgacac aaatggtcag atggtcatga agagcaaaca gcagttaaaa
     8641 gaagattttg gtatctcagc aaagcctatt gccatgttta agtaagtggt tcatcccctt
     8701 tttgccgcgg ctatgaaggg gatagccaac aatgacccca cgactcatct caattatctc
     8761 gccatgacct ggctaagtcc cagtccgatc tcttgccgtc gcgttcacgc ctgaatgact
     8821 tctcctgaac ttaagtctca gacctatttg gccggtaatc cctctcaaat ttgctccttt
     8881 tgcgttccgc cttacatcaa cctgtaacag aaccagccat tttctgcgta attgtgatct
     8941 gagttgtaaa acgcagatta ttatctgtgt tttacatgtt taatgttgac ctcaaaaatc
     9001 atgtgggtaa gtatgatgac catctaagca aaacaaaaca cagaggatga cgcaatgaaa
     9061 gcactggctc ggtttggcaa agcctttggc ggctacaaga tgatcgatgt gccacaaccc
     9121 atttgtggcc cggaagatgt agtgattgaa attaaagccg cggcaatctg cggcgcagac
     9181 atgaagcact acaatgtcga tagcggttct gatgagttta actctatccg cggccatgag
     9241 ttcgcaggtt gtattgcgca ggttggtgaa aaagtcaaag actggaaagt ggggcaacgc
     9301 gtcgtgtcgg ataacagcgg tcacgtctgc ggcgtttgtc cggcctgtga acaaggtgat
     9361 tttctgtgtt gtacagaaaa agtaaacctt ggtctggata acaatacctg gggcggtggt
     9421 ttttccaaat attgtctggt tcctggtgaa attctcaaaa ttcatcgtca tgcgttgtgg
     9481 gaaatccctg atggtgttga ttatgaggac gcagccgtac ttgaccctat ctgtaatgcc
     9541 tacaaatcca tcgcgcagca atcgaaattc ctccctggtc aggatgtggt cgtcatcggc
     9601 actggcccac tcgggctgtt ctccgtacaa atggcgcgaa ttatgggggc ggtaaatatc
     9661 gtcgtcgttg gtctgcaaga agatgtggcg gtccgcttcc cggttgcaaa agaactgggt
     9721 gcgacggcag tagtaaatgg ttctaccgaa gatgtggtgg cgcgctgcca gcaaatttgt
     9781 ggcaaagaca atctgggact ggtgattgaa tgctccggtg ccaatatcgc actgaaacaa
     9841 gccatcgaaa tgctccgtcc gaacggggaa gtggtacgcg ttggaatggg cttcaaacct
     9901 cttgatttct cgattaatga cattaccgcc tggaacaaaa gcatcattgg gcatatggcc
     9961 tatgactcca cctcatggcg taacgctatc aggctattag ccagcggcgc tatcaaagtc
    10021 aaaccgatga tcacgcatcg tatcggcctg tcgcaatggc gcgaagggtt tgatgcgatg
    10081 gtcgataaaa ccgcaatcaa agtgatcatg acttacgact ttgatgaata accactctcc
    10141 tgaaggaacg ttatcgcatg gatcagataa caaaaccgca ttgtggtgca cggctggatc
    10201 gcttaccgga ttgccgctgg cattcatcaa tgtttgccat cgtcgcgttt ggcttgcttg
    10261 tctgctggag taatgccgtt ggtggcttga tcctcgcgca gctgaaagcg ttgggctgga
    10321 cagataattc caccacagcc acattctcag caatcacgac cgccggaatg tttctcggtg
    10381 cgctggtggg cggcatcatt ggcgacaaaa ccggtcgcag aaatgcgttc atcctctatg
    10441 aggccattca tattgcctcg atggttgtcg gtgctttctc accgaatatg gatttcctta
    10501 ttgcctgccg ttttgtgatg ggagttgggc tgggagcttt actggttacg ctgtttgctg
    10561 gtttcaccga atatatgccc ggtagaaatc gtggaacgtg gtcaagtcgg gtttccttta
    10621 ttggcaactg gtcatatccg ctctgttcat tgatagcgat gggactcacg ccgctgatta
    10681 gtgcagagtg gaactggcgg gtacaactgc ttatccctgc aatattgtcg cttatcgcta
    10741 cggcgctggc ctggcgctac tttcctgaat ccccgcgctg gctggaatcg cgcggacggt
    10801 atcaggaagc cgagaaagtc atgcggagta tagaagaagg cgtcatacgc catacaggta
    10861 aacctttgcc gcccgtggtt attgctgatg acggtaaagc accacaagcg gtgccatatt
    10921 cagccttact gacaggagta ttactgaaac gcgtgatatt aggctcttgt gtgctgattg
    10981 ccatgaacgt tgtgcagtac acactaatta actggttgcc aacgatattc atgacccagg
    11041 ggattaattt aaaagactcg attgttttaa ataccatgag tatgtttggc acgccatttg
    11101 gcatttttat tgccatgctg gtgatggata aaattccgcg taaaacaatg ggtgtggggc
    11161 tattaatcct gattgcggtg ctcggatata tctattcact gcaaaccagc atgttgctca
    11221 taacgttgat tggtttcttc ctgattactt tcgtctatat gtacgtttgc tatgcctcgg
    11281 cagtgtatgt ccctgagatc tggccgacag aggccaaact ccgtggctcc ggtctggcga
    11341 atgcggtagg gcgaatcagt ggtattgccg caccttatgc cgttgcagtg ctgctcagta
    11401 gttatggcgt tacgggagtc tttattcttc tgggggcggt ttcaattatt gtcgcaattg
    11461 ctatcgccac cattggaatc gaaaccaaag gtgtctccgt tgaaagttta agtattgatg
    11521 cagtagccaa taaataatac gggtggattc aaaatgaaaa attcaaaagc aatattgcag
    11581 gtgccgggca caatgaaaat tatttcagca gaaataccag tgcctaaaga agatgaagtt
    11641 ttgattaaag tagaatatgt cggtatttgt ggttcagatg tacatggttt tgaatcaggt
    11701 ccgtttattc cgcctaaaga cccaaatcaa gaaattggcc tgggtcatga atgcgccggg
    11761 acggttgtgg ctgtgggaag ccgtgtgcgc aaatttaaac cgggggatcg ggtaaatatc
    11821 gaacctggcg ttccttgcgg tcactgtcgt tactgtctgg aaggcaaata taacatctgc
    11881 ccggacgttg attttatggc gacacaaccc aactaccgcg gcgcattaac gcactatctg
    11941 tgtcatccgg agagctttac ttacaaactg cccgacaata tggacacgat ggaagggacg
    12001 ctggtggagc ctgccgcagt cgggatgcat gccgcgatgc tggcagatgt taaaccgggt
    12061 aagaagataa ttattctggg agcaggttgt attggtttga tgacgttgca agcgtgcaaa
    12121 tgcctgggag caacggaaat tgccgtcgtt gatgtgctgg aaaaacgtct ggcaatggcg
    12181 gaacagcttg gtgcgacagt ggttattaac ggcgcaaaag aagacactat tgcacgctgt
    12241 cagcaattta ccgaagacat gggcgcagat attgttttcg aaacagcggg ttctgcggtc
    12301 accgttaaac aggcacctta tctggtaatg cgtggcggta aaattatgat tgttggtact
    12361 gtacccggcg cttcggcaat caatttcctc aaaatcaatc gcgaagtcac tatccagacg
    12421 gtattccgct atgccaatcg ttatccggtc acgattgaag caatttcttc agggcgattc
    12481 gatgtgaaat cgatggtgac gcatatttac gattatcggg atgtacaaca ggcatttgaa
    12541 gagtcagtta acaacaaacg cgacattatt aaaggcgtta ttaagattag cgattaatta
    12601 atacgggaga acagaacatg ctcgcagata tcagatattg ggaaaacgat gccacaaata
    12661 aacgttatgc tattgcccat tttaatgtat ggaatgcaga aatgttgatg ggcgttatcg
    12721 acgcagccga agaagcgaaa tcaccggtta ttatttcttt tggcacaggt tttgtcggca
    12781 acacctcatt tgaagatttc tctcacatga tggtatctat ggcacaaaaa gcaacggtgc
    12841 cggtaataac tcattgggat catggtcgga gtatggagat tattcataac gcctggactc
    12901 atggtatgaa ttcattaatg cgtgatgctt ccgcatttga tttcgaagaa aatattcgtt
    12961 taaccaaaga ggctgttgat ttcttccatc cgctgggtat tccggtagag gcggaattag
    13021 ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc gggctatcac tataccgatc
    13081 ctgaccaggc ggctgaattt gttgaacgta cgggctgtga ctcattggct gtcgccatcg
    13141 gcaaccagca tggggtttat acgtcagagc cacaattgaa ctttgaggtc gtcaaacgcg
    13201 tacgcgatgc agtgtctgtt ccgctggttt tgcatggcgc atcggggatc agtgatgccg
    13261 acattaaaac tgcaatttcg ttgggtatcg cgaaaatcaa catccatacg gagctctgtc
    13321 aggccgcaat ggtcgcagtg aaagaaaacc aggatcagcc tttcctgcat ttagaacgtg
    13381 aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat taaactgttc ggttctgatg
    13441 gcaaagcgga ataatgatat ggataatctc gacgttattt gtataggtgc cgctattgtt
    13501 gatattccat tgcaaccggt cagtaaaaat atctttgatg tggattctta tcctcttgaa
    13561 agaatcgcaa tgaccaccgg gggtgatgca ataaatgaag caacaattat ttctcgtctg
    13621 ggccattgta cagcattaat gagccgtatt ggtaaagatg ccgcagggca gtttattctc
    13681 gaccattgcc gcaaagagaa tattgatatt caaagcctga agcaggacgt caacatagat
    13741 acctctatta atgttggact ggtgacggag gatggcgagc ggacgtttgt cacaaaccgt
    13801 aacggcagtc tgtggaaatt gaatattgac gatgttgatt ttgcgcggtt ttcccaggcg
    13861 aaattattat cactggccag tattttcaac agtccactat tggatggtaa agcgctaaca
    13921 gaaattttta cgcaagctaa agcccggcag atgaatatct gtgccgatat gattaaaccg
    13981 cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcaa ttatgccgag
    14041 gcaaaattac tcaccgggaa agagacactg gatgaaattg ctgacagctt tcttgcgtgc
    14101 ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct gctttatcaa gcgtggtgac
    14161 atgacgatga aggtgccggc ggtcgcagga ataaccgcca tcgacaccat tggcgcgggc
    14221 gataactttg cttcaggttt tattgcggca ctgttagaag gaaaaaatct gcgtgaatgc
    14281 gcacgctttg ccaatgcaac ggcggctatc tcggttctaa gcgtcggtgc caccaccggc
    14341 gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat acgaaggata atgaaggcaa
    14401 atgaaaaaga tacctttagg cacaacggat attacgcttt cgcgaatggg gttggggaca
    14461 tgggccattg gcggcggtcc tgcatggaat ggcgatctcg atcggcaaat atgtattgat
    14521 acgattcttg aagcccatcg ttgcggcatt aacctgattg atactgcacc aggatataac
    14581 tttggcaata gtgaagttat cgtcggtcag gcgttaaaaa aactgccccg tgaacaggtt
    14641 gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag gaagtttatt caacaaagtt
    14701 ggcgatcggc agttgtataa aaacctttcc ccggaatcta tccgcgaaga ggtagaagcc
    14761 agcttgcaac gtctgggttt tgattacatc gatatctaca tgacgcactg gcagtcggtg
    14821 ccgccatttt atacgccgat cgctgaaact gtcgcagtgc ttaatgagtt aaaggctgaa
    14881 gggaaaattc gcgctatagg cgctgctaac gtcgatgctg accatatccg cgagtatctg
    14941 caatatggtg aactggatat tattcaggcg aaatacagta tcctcgaccg ggcaatggaa
    15001 aacgaactgc tgccgctatg tcgtgataat ggcattgtgg ttcaggttta ttccccgcta
    15061 gagcagggat tgttgaccgg caccatcact cgtgattacg ttccgggcgg cgctcgggca
    15121 aataaagtct ggttccagcg tgaaaacatg ctgaaagtga ttgatatgct tgaacagtgg
    15181 cagccacttt gtgctcgtta tcagtgcaca attcccactc tggcactggc gtggatatta
    15241 aaacagagtg atttaatctc cattcttagt ggggctactg caccggaaca ggtacgcgaa
    15301 aatgtcgcgg cactgaatat caacttatcg gatgcagacg caacattgat gagggaaatg
    15361 gcagaggtct tggggcgtta aatactcaac acgattcacc tctgtttctg tgaaaactgt
    15421 gatgaaaatc tgtgattcaa acaggttatt ttgaaagtaa acatcggtta tgcgataatc
    15481 gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg gcggcaaaag acaggattca
    15541 ggcaattaag caaatggttg ccaacgataa aaaggtgaca gtctcaaatt tgagtgggat
    15601 ttttcaggta accgaagaaa ccattcgccg cgatcttgag aagctggaag atgaaggctt
    15661 tttgaccaga acctatggtg gtgctgtttt aaatacagcg atgttgacgg agaatatcca
    15721 tttttataag cgcgcttcat cgttttatga agagaagcag cttattgcac gcaaggcact
    15781 accctttatc gacaataaaa ccaccatggc agccgattcc agtagtaccg taatggaatt
    15841 gctcaaattg ttacaggacc gtagtgacct gacgttgcta accaactcgg cggaagcgat
    15901 tcatgttctg gctcagtcag aaattaaagt cgtttcaacg ggtggggaac taaacaaaaa
    15961 tacactttca ctgcaaggaa gaattactaa agagatcatc agccgctatc atgtcgatat
    16021 catggtaatg agttgtaaag gtcttgatat taacagcggc gcgctggact ctaatgaagc
    16081 ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca gaagttgcgt tattggttga
    16141 tcactctaag tttgatcgca aagcttttgt ccagttagcc gactttagtc atattaatta
    16201 cataataact gataaatcac cgggtgcaga gtggatagca ttttgcaaag acaataatat
    16261 ccagctggtg tggtaattta agcacgcgtt gtcaatatta tttgttcctg cgcctggaaa
    16321 taaaatgatg ataaagatgc ggcaagtgaa tttgccgcaa ttgctacctt ttttagcaaa
    16381 tccgaggcac ccaatggaac aatatgatca aattggcgca agactggacc gcttgccttt
    16441 ggcccggttt cattatcgta tatttggtat tataagcttt agtctgttat taacagtgaa
    16501 ccgccccggt tttcctggag agtgttttat ctgtgaactc aggctgccag atcatcgttt
    16561 ctgatggaag cataataagc tttttctgct tctgccggag ggatatgacc cagccttccc
    16621 agcaatcgtc gattgttata ccagtccacc cacgtgagtg tggccagttc cacttctgca
    16681 cggtttttcc agctcttacg gtgtattacc tccgctttgt aaagaccatt gatgctctcc
    16741 gccatcgcgt tgt
//
LOCUS       DAZFKY010000102        16653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFKY010000102 DAZFKY010000000
VERSION     DAZFKY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..248)
                     /locus_tag="ACHKCL_002949"
     CDS             complement(96..248)
                     /locus_tag="ACHKCL_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5763876.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(512..1246)
                     /gene="cysH"
                     /locus_tag="ACHKCL_002950"
     CDS             complement(512..1246)
                     /gene="cysH"
                     /locus_tag="ACHKCL_002950"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5763877.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1320..3032)
                     /gene="cysI"
                     /locus_tag="ACHKCL_002951"
     CDS             complement(1320..3032)
                     /gene="cysI"
                     /locus_tag="ACHKCL_002951"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5763878.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3032..4831)
                     /gene="cysJ"
                     /locus_tag="ACHKCL_002952"
     CDS             complement(3032..4831)
                     /gene="cysJ"
                     /locus_tag="ACHKCL_002952"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5763879.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5150..5512
                     /gene="queD"
                     /locus_tag="ACHKCL_002953"
     CDS             5150..5512
                     /gene="queD"
                     /locus_tag="ACHKCL_002953"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5763880.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5590..6861
                     /gene="ygcN"
                     /locus_tag="ACHKCL_002954"
     CDS             5590..6861
                     /gene="ygcN"
                     /locus_tag="ACHKCL_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5763881.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6852..7112
                     /gene="ygcO"
                     /locus_tag="ACHKCL_002955"
     CDS             6852..7112
                     /gene="ygcO"
                     /locus_tag="ACHKCL_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5763882.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7129..7704
                     /gene="ygcP"
                     /locus_tag="ACHKCL_002956"
     CDS             7129..7704
                     /gene="ygcP"
                     /locus_tag="ACHKCL_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5763883.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7852..8712)
                     /gene="ygcQ"
                     /locus_tag="ACHKCL_002957"
                     /pseudo
     CDS             complement(7852..8712)
                     /gene="ygcQ"
                     /locus_tag="ACHKCL_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8709..9488)
                     /gene="ygcR"
                     /locus_tag="ACHKCL_002958"
     CDS             complement(8709..9488)
                     /gene="ygcR"
                     /locus_tag="ACHKCL_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5763884.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9466..10802)
                     /gene="ygcS"
                     /locus_tag="ACHKCL_002959"
                     /pseudo
     CDS             complement(9466..10802)
                     /gene="ygcS"
                     /locus_tag="ACHKCL_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10896..12350)
                     /gene="ygcU"
                     /locus_tag="ACHKCL_002960"
     CDS             complement(10896..12350)
                     /gene="ygcU"
                     /locus_tag="ACHKCL_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5763885.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12420..13205)
                     /gene="ygcW"
                     /locus_tag="ACHKCL_002961"
     CDS             complement(12420..13205)
                     /gene="ygcW"
                     /locus_tag="ACHKCL_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5763886.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13524..14801
                     /gene="yqcE"
                     /locus_tag="ACHKCL_002962"
     CDS             13524..14801
                     /gene="yqcE"
                     /locus_tag="ACHKCL_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5763887.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14828..16306
                     /gene="ygcE"
                     /locus_tag="ACHKCL_002963"
     CDS             14828..16306
                     /gene="ygcE"
                     /locus_tag="ACHKCL_002963"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5763888.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLSTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3968 a         4389 c         4182 g         4114 t
ORIGIN      
        1 actatatatt cacataaaaa atataaattt aaaaaagggt acaggagacc aaaccatcac
       61 aattcaatac gcggggtttt acccccgcgt ggtggctact tcttcgattc gtaagcgaga
      121 actgccttaa actccatacc acgctctctg atactcaact cacataacga gtcgcctacc
      181 atgagcgtga atcccagtac tgtacaacac agtactatga ttgccacaag ggcatatttt
      241 gttagcatgt cttgcctccc tgcaaagaag agactaacat ccgaattgct acgttcgaac
      301 gcgagcctca ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa
      361 tgcttaacgc cagacagcct caagcacccg acgccattct atacctgata attcttccag
      421 cggcgttcac gtttaaaatc aggaatgctg ctcgcaaaat aaaaacgccc ccatcattac
      481 tgacagaggc gtttaattta tccggcaata tttacccttc gtgtaaccca cattcccttt
      541 ttaagccaaa gaaacgcgtt tcttcttccg ccatgccggg ttcccattta cgggttgtat
      601 gggtatcgcc caccgataaa tatccttcgt cccataatgg gtgatatttc aggccattct
      661 tttgcagata ctgatatatc gttcggttat cccagtcgat aatcggcagc actttaaata
      721 cgccacgctg aattgccagc accggtaaat tggcgcgact gccggattgt tcacggcgca
      781 ggccagcaaa ccaggtttgc gcgttaagtt ctttcagcgc ccggttcatc ggttcgactt
      841 tgttgatgtc attgtacttt tcaatgcctt caacgccctg ttcccacagt tttccgtagc
      901 gtgcttcctg ccaggctgcg ctttcggtag cacggtacac tttcaggttg agcttgagtt
      961 tgtccgttaa ctcgtcaata aagcggtagg tttccgggaa caagtaaccc gtatcggtga
     1021 ggatcaccgg aatatccggg cgaatttgat tcaccagatg caggctcacc gccgcctgaa
     1081 tgccaaagct ggaagaaagt acatattcac cgggcagatt atccagcgcc caggctacgc
     1141 ggccttcagc gtccagtttt tccagttggg cgttagtttc cgccagcgcc agaatgcgat
     1201 ctaccttcgg cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt
     1261 ttgccttgcc tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt
     1321 taatcccaca aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta
     1381 aagtcgccga agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca
     1441 agcgacgcca ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc
     1501 ccaatgcggt tgccgccaag atgcaggttg tagcgacccg gcgctttacc caccaggccc
     1561 acttccgcca gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata
     1621 tgctcatcgc tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc
     1681 aggaaacgct ccgcttccgc catcgccagc gggcaagtcg ggaacgagac gcaggccatt
     1741 gagttttcac gctgcggcgt gacggcattc attaacccgc tctctttggc gatcttctcg
     1801 atcttcgctt tctcgctttc cggcacaccg gcgatgatca gattctggtt cgccgtaatg
     1861 cggaaatcac ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc
     1921 ggataatcaa ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg
     1981 cccttaaccc agccaatacg atctccgcgt ccggtgaact catacggacg gatcggttca
     2041 aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc
     2101 agcgtgtatt tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt
     2161 gtcacgacgg cttccgccac tgccagcgta tgctccagcg gcagatagcc aaactcactc
     2221 gccgtgcggg cgtaggtttt cttgttgccg tgttcgatag aaagtccacc gcccaccaac
     2281 aggttaaagc ccaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggcg
     2341 tgcagatcga tatcgttctg tggcgggatc actaccgtgg ttttgaattt acgcggcagg
     2401 taggtctggc cgaggatcgg ttcttcatcg gtggtggcga ctttttcctg atcgagccag
     2461 atctccgcat acgcgcgggt acgaggcagc agatgttcag agattttctt cgcccactcg
     2521 tacgcttctg cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc
     2581 atgtcgttag cggtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt
     2641 ttgacgttct ttttcagaat gccgtggaac tggaatgtct ggcggttggt caggcgaatg
     2701 ctgccataga tggtgttttc accggcaaat ttatcgatcg cctgccactg tttggtggtg
     2761 ataactccac ccggcagacg gcagcgcaac agcatcgcat ggcgcggctc cagcttctgt
     2821 tcagcacgtt cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc
     2881 agcaggaagt tatcgccctt aaagccgccg gtcaggccgt cgtttaaatc ttccgcaatg
     2941 gtgccgcgca ggtagttact ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg
     3001 accactaaag gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct
     3061 ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg
     3121 caatcacttc cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga
     3181 cataaatgtg ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt
     3241 tgtcttgtac gtaaactttt tctttttgat cgcgcgacca ggcaagatcg atacgcgtca
     3301 gcacgccctc tttgacgtaa cgctgccact ccacctggta gaggaaatct tccgtaaagt
     3361 gcgggttgcc aaagaacagc cagtttttac ccggcgcttc gtcagcggcg cgctgctgca
     3421 taaaggcgcg gaacggcgcg atgccggtgc ctgggccaat cataatcacc ggggtttccg
     3481 ggttagcggg caggcggaag ttatcgttat gttcgataaa tacgcgaact tcgccctctt
     3541 cttccacgcg gtccgcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt
     3601 cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc gacttcctcc tgcgaggagg
     3661 cgatggaata cagacgcggc gtcagcgggc gcagcagatt aatcagcgct tcggcgtcca
     3721 gttgcgccgg agagaagcgc accatgtcga caatcggcgt agtcgcggcg taatgctgta
     3781 acttcgcttt atcgcccacc agcggcaaca gcgtttcgct gcgggtaagc gtggcatagt
     3841 tctcaacaat attggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca
     3901 gaggcaacgt tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga
     3961 caagttcttt caccagtgcc ggatcgttct gataccagac acccagcgcg tcacccggct
     4021 ggtaacgcag gcccgagtca cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt
     4081 tacgcccggt aattttctgg ttaaccgaaa ggctcgctac cagcggcgcg tctttgctgt
     4141 acgggctggt gtggatttca tttaccacgc cagtagcgac ggattgcgaa ggtgccgcga
     4201 caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct
     4261 ggtattcaac gtcggcgtcg acacggtcga gcaggcgttc accgcccagt tccgccagct
     4321 tgctgtcgaa atctttcccg gactggcaga aaaattcata agaactatcg ccgaggctaa
     4381 acacggcaaa agcggtattt tccagcttcg gcgctttttt ggagaacagg aacttatgca
     4441 gcgcgacggc ttcttccggc ggttcccctt ctccttgcgt tgacgtcacc acgataagca
     4501 gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca
     4561 gttttgccgt caataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt
     4621 gcgaagcaga gataatagtt atacccggca tttctgcggc tggcgctggc gtcgctgcaa
     4681 gcgcagcagg ctgctgattg agcacgcccc agaaatagcc agaaacccag gcaagctggg
     4741 tgggagttaa atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa
     4801 gcaacgcgga aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg
     4861 tttctgcgcc ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg
     4921 gtgtggatta aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca
     4981 atagtcttta acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa
     5041 tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaga
     5101 gcctttccgg tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac
     5161 gttatttaaa gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca
     5221 taaatgtggt cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt
     5281 cgatccgcat acgggctgga ttatcgattt cgctgaacta aaagcggcgt ttaagccaac
     5341 ttatgagcgc ctcgatcacc attatctcaa tgatattcca ggtctggaaa acccaactag
     5401 cgaagtttta gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc
     5461 ggtgatggta aaagaaacct gcaccgctgg ttgtatctat cgcggcgaat gataagagtg
     5521 tggcggcggt caatttccct taagtaacgc tatgttaggg tgttgtgttc aggatatctg
     5581 gggcatgaca tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc
     5641 gcttgcgcgt tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa
     5701 atccccggca gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc
     5761 ctccctcaat ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc
     5821 ctgttaacgc cggattgcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg
     5881 agtgtattac gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc
     5941 gaatgcatcc ctggtgcgac ggtggatgca ctgtatgaag aaaacggcag ggtgtgtggt
     6001 gtcatttgtg gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac
     6061 agcgtcctgg ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg
     6121 gggatcaaag aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag
     6181 aataacgaag gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc
     6241 gcatttcttt atactaatca acaaacgctc tcgttaggga ttgtttgccc actctcttcc
     6301 cttacgcaaa gtcgtgttcc ggcaagcgag ctgcttgctc gctttaaaac gcatccggca
     6361 gtgcgcccgc ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa
     6421 ggtggcttgc acagtatgcc ggtacaatac gccggtaacg gctggctgct ggtgggcgat
     6481 gcgttgcgca gttgcgtcaa taccggaatt tctgtgcgcg gcatggatac ggcgctaact
     6541 ggcgcgcagg cggcggcaca aacactgata agcgcctgcc agcaccgcga gccgcaaaat
     6601 ctgtttccgc tttatcatca caacgtagag cgcagcctgc tgtgggatgt tctacagcgt
     6661 tatcagcatg ttccggtgct tttgcaacgc ccgggatggt accgtacgtg gcctgcgtta
     6721 atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc
     6781 cagttactct ggcgtcattt acgtcgtcat ggcttgtgga atctggcggg cgatgttatc
     6841 aggagtgttc gatgtctgta gcccgtaatc tctggcgcgc tgctgatgcg ccgcacattg
     6901 ttccggctga ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggtag
     6961 gtcttttttc gctcacaccg gaaggtgact tacgcgttga ttatcacggc tgcctggagt
     7021 gtggcacctg ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt
     7081 ccggattcgg catcacctac cgatttggat aaataataag gataatttat gcccctctta
     7141 cacctgctcc gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg
     7201 gccatcgatt ctgaatgcca gtttatttcc gtcttgtacg gcaatatctg caccattagt
     7261 caaatcgtta aaaagattaa gaacgctggg aaatatgctt ttattcatgt tgatttgctg
     7321 gaaggcgcgt caaataaaga agtggtgatt cagtttttga aactggtgac cgaggcggat
     7381 ggaattatca gcaccaaagc atcgatgcta aaagcggcaa gagcggaagg ttttttctgt
     7441 attcatcgcc tgtttattgt tgattcgatt tcgtttcaca acattgataa gcaagttgcg
     7501 caatcgaatc cggattgtat tgagatcctg ccaggctgca tgcccaaagt gctgggctgg
     7561 gtgacggaaa aaatccgcca accgctgatt gctggcgggc tggtatgcga tgaagaagat
     7621 gcgcgtaatg cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggagtctgg
     7681 acgttagcga aaaaattgct ttgacgggat aatcgtaacc aattgaattt ggtttgattt
     7741 gtaggccgca cgccacatcc gacattcagc gcctgatgcg acgcttaaca cgtcttatca
     7801 ggcctacagg tccccagtcc agaaccgtag gcaggatcat ccggcagcac gttattggca
     7861 ttctgcgtca atgtttgtca ccagtgcctc cagcatcact ttccagtcat caaccacgcc
     7921 aacatctgcc tgtgaaaata ccgctgcgct ggcgtcatga ttaatcgcca ccacaaactt
     7981 gctattgcgt acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac
     8041 ctcaggtgcc agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt
     8101 catcactcgc gcccgactgt agcccacttc ggctcccagc ttttatgcca gcatagcgat
     8161 ttcctggtta tctgcctctc cgccctgccc caccaccaac acccgtctgg cctcagctaa
     8221 tggatcgcgg gtaacatttt tcaggttctc tatgctaacc agccagtccg gaagcgcacc
     8281 tgggactatg ttcagttgct gcataccaga gggcaacgtg gcatttttat ctgctcccgc
     8341 ctggcgcgcc agggaaagac atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc
     8401 atttccccag tgggatttgc gcacgcttac gattggaatc tctaacgaag tcacctggca
     8461 gatgcttgca ccatgtaaac gccaggccag ccgagtcgac agttcatcgc caaacgttcc
     8521 cggcggaaag agaacaacgt tcgcaggcgt tcgctgccac tgttccacca gcgcatccag
     8581 cacctgttcc accactacgg gctgtggttc tatttgccag tgcgccaggg tacaaccgct
     8641 gaaatcctgc gccgccagcc agcccgctaa tgcggcattc tcctgattta ttgtcacaat
     8701 tgcgatattc atggctgcat cctttggcgt aaatagtcct gccatagctt ttgcgctttt
     8761 tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt
     8821 tgcagacact gcatcgctgg caattcggca gcaacagttt tacggataat ttccgctttc
     8881 cccgcagcca ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc
     8941 acggcaatca ccgcaggcaa gcgaatccga caacagcgca gcccatgttc agtacgctgt
     9001 tcgagggtga taaacggcgc gtcgagagtg aaacgctcca cctgggtgaa gcaaggccag
     9061 cccagcattt ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag
     9121 ccggtaatta tcagatccag cggattctga cgctgccatt cggcaatatg gcgagcgaca
     9181 aattccgacg caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca
     9241 agagccatga gatagcgcag ccagtgcaac gcccgttcat cccccatgct caacgcggtt
     9301 aaagacatcg gcgtaccgtt ttttctctgc gccaacagca gcgcggcggc agcctgttca
     9361 tcagcaccga gtaaacttcg cagtagcgaa atatccggtc cgctattacc atgagccgcc
     9421 gcctgccact ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga
     9481 atgttcattc gcacctccta catttcccgc cgccaccagc gggagtgctt tggtttctgg
     9541 tgcccatagc caggttacaa caaaaccagc caacaacact gccgccagga gcaataaggt
     9601 gacttgcatt ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcccc
     9661 caaccgactc atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact
     9721 ttccgcaggc aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac
     9781 aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccatcactac cagcgttgcc
     9841 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
     9901 aatcccagca gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca
     9961 tcttccagtc caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
    10021 attacgaggc agacaaagaa gacgctgtta aacgccgtgc gccgccagta gcgcgaagag
    10081 aacaaggttt tgatgtgttt atgggtcgcc gttgccactt catcgcccag taaaacatgg
    10141 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc
    10201 cagcgtggcg attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct
    10261 gccgatgcca gcagccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca
    10321 atacttgcca acacatagcc tacggtccac accacgctga atgcgcccag caaaacaccg
    10381 cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac ctactgagta atcgcctccc
    10441 agaccaatgc cgatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat
    10501 tgcaagaacg aagcgagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca
    10561 atatggtcgg agatccaccc aagaaccagg ctgccaagaa acaaaccaag gtgcgccgag
    10621 ccgccgatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ttgaataatg
    10681 gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
    10741 gcaattcgac agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc
    10801 atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
    10861 caacgcccca tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta
    10921 cccgtattca taatgccatt aggatcgaac tgctttttca gcccttccag caacgcccac
    10981 gcgctgccgt gttccagctt gctccagtga acgcgatgtt taccgatacc gtggtggtgc
    11041 accatcgaac caccgaggcg aatggtttct tcgcaaatga ttttgttgag cggattgtgg
    11101 tacttgtcga tttcctcttc cggcttacag ttgacaacgt tgtagtcgta gacgaagtac
    11161 atgttggtgc cgttctgata gctatgagag gagtgaccgc ccagcatggt gatgtcgtcg
    11221 gcgtgcggga actcggtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag
    11281 ctccagcagc cggacacttc ggtagtaaag cccatgttgc cggttttgag gatctgcaca
    11341 cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagc
    11401 ttgctgtcca cgcgttggca ttgcgggtag cgggcaacga tttccgcaat cccttcgccc
    11461 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacgca ttttccgtcg
    11521 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
    11581 cgataccctt ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg
    11641 atatagccgt agaagaggtt gttttccggg gtgaatttaa agattttcac tgttacttca
    11701 gtgatatagc acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc
    11761 gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat ctgccaacac tgcctccaga
    11821 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
    11881 gctaccaggc cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa
    11941 cctttttcac gcaacgcgtt ttccagcacc tccagcggaa caccacattg cgccgtcgcc
    12001 tgcatattct caatatcaat attaatgatt tgattcatgg cggaaccgtc gagcaccacc
    12061 gagttttcta caacagtttc cagcccacct tcggtggcgg aagcaccggt acgcggcaca
    12121 ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttgctc tgtggaaccg
    12181 agttttacga ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaattta
    12241 cgaaaacggt caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca
    12301 ccaacaattt cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct
    12361 tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatgggt
    12421 tagcgcacta aataaccgcc atcgaccact aataaatgcc cgttgacata attcgatgcc
    12481 tgacttgcga gaaacacggc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc
    12541 ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaatg
    12601 tctgttgcat aatagccagg ggcgatacca tttacctgaa tattatattg acctagttca
    12661 tcacaataag ctttggtgaa tccggcaaga gcatgtttag tagcagaata tgcaggtgac
    12721 cattgtccac ctaagtaaga gaacaatgaa cagatattaa tgattttacc gcttttctgc
    12781 gggatcataa tttttgccgc ttcgtagctt aactcgaatg cggcggtcag gttcacatca
    12841 atcatcgggt cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg
    12901 gcattgttaa ccagaatatc aactgtaccg aaacgctcac agcaggcagc gataatcttc
    12961 tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgattt
    13021 tcaatcattt cctttgtttc gccgttatct ttgacaaaac ttggaataaa gacatttgcg
    13081 cctgctttgg ccaacgccat ggcaaatgcc tggcctaaac cgctattccc gccggtaaca
    13141 attgcggttt tacctttcag ggagaaaaaa tccattgaga acgcattgag agattcgatt
    13201 gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag
    13261 agggaagtta cctttctcat tatctctggt aactggagat aatttttagc ataaggacat
    13321 aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tatcaaatag ttttaaaaag
    13381 cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttat tatttcaatt
    13441 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
    13501 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
    13561 cgataattag ttttagcggc ggcgttagtt tcgacctggc ttatttacgt tatatttatc
    13621 aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta
    13681 cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt
    13741 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
    13801 caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacaacga
    13861 ttttaatgct gtggtcggtg tcgattaaag ccgcatcgtt gcttggcgat catagcgagc
    13921 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcag
    13981 tgtttaccat gtgggttttt tctcgctttg caccggatga cagcaccagc ctgaaaacgg
    14041 tcattatcat ctatagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
    14101 gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
    14161 acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggcg
    14221 tcttcaccat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtatggca
    14281 tgtcgctggt ggcggcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
    14341 gcggcccact tggcggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga
    14401 tccaaatcct ttccgtactc ggcctgctgg cgttaactgc cctgctcgtc acgaactcta
    14461 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
    14521 cctcacgcgg gctgtactgg gcctgccctg gcgaagcgag aacaccgtct tacattatgg
    14581 gcaccacggt aggtatttgc tcggtgattg gattcctgcc ggatgtcttc gtttacccaa
    14641 ttatcggcca ctggcaagac acccagcccg cagcagaagc ctaccgcaat atgtggctga
    14701 tgggcatggc ggcgcttgcc atggtgattg tctttacctt tttgctgttc caaaaaattc
    14761 gtactgctga tagcgccccc gcaatggcca gcagcaagta agccaggaaa attacaggag
    14821 aaaaatcatg tcgaagaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt
    14881 ggtgatgtac gatctggaag gtaacgtggt ttgcgaaggc aaaggtttat tgcagccgat
    14941 gcacacgcca gatgccgata ccgcagaaca tcctgacgac gatttatggg catcattatg
    15001 ttttgccggt cacgatttga tgagtcagtt tgccgggaat aaagaagata ttgtcggtat
    15061 tggtctggga tccatccgtt gctgccgtgc gttattgaaa gccgatggca cgcctgctgc
    15121 gccgttgatt agctggcagg atgcacgcgt aacgcgtccc tacgaacaca ctaatcctga
    15181 cgtggcctat gtcacctctt tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa
    15241 agacaatatc gccaactatt ttggtcagtg gccggtggat tataagagct gggcatggag
    15301 cgaagatgct gcggtaatgg agaagtttaa tatcccccgt catatgctgt ttgatgtgca
    15361 aatgccaggc actgttcttg gacagatcac accacaagcc gcgctggcga cacatttccc
    15421 ggcagggctg ccggttgttt gtaccaccag tgataaaccg gtagaagctc tgggagccgg
    15481 attactggat gatgaaacgg cggtgatttc actcagtact tatatcgcgc tgatgatgaa
    15541 cggcaaagcc ttgccgaaag atccggtggc gtactggccg attatgtctt ctattccgca
    15601 aacattgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga gctggctgcg
    15661 cgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt caccggaaga
    15721 tttactcaac aaaaaagctt cttgtgtgcc acctggctgt aatgggctga tgacggtgct
    15781 ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg gctttgattc
    15841 cagcatggat tacgcatgga tatatcgttc gatactggaa agcgtggcgc tgacgctgaa
    15901 gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaagcatg tgatcattac
    15961 tggtggcggt tcgaacagcg atctgtttat gcagattttt gccgacgtgt tcaaccttcc
    16021 ggcacgacgt aacgccatta acggttgtgc aagtctgggg gcagcgatca atacggcggt
    16081 aggtctgggg ctatacccgg attacgcaac ggctgtcgac aaaatggttc gcgtgaaaga
    16141 tatctttata ccgattgaga gcaatgccaa acgctacgac gcgatgaata aaggtatttt
    16201 caaagcccta accaaacata ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg
    16261 ggaattgggg aatgcggatt cgatccagag ctggtcgaat gcgtaagtga ggaagaccgg
    16321 gcgggaaact gcccggcctg aacatacctg aatggttatc cccgctggcg cggggaacat
    16381 aagttcgaca acttaaaaaa tgataacaag tccggtttat ccccgctgat gcagggaaca
    16441 taataaaaac tttaactagt tcggtttatc cctgttttta caaggaatat taactacact
    16501 cgatgtcttt agaataaaag acaatacgaa tcgcacttta tcaattttca cttaaagtat
    16561 gaactggata tctttattca taagaactat tcatcaacag cgtatagagg cggttatgta
    16621 aaaccactca ttagcctcaa aacaaagaca aat
//
LOCUS       DAZFKY010000103        16516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFKY010000103 DAZFKY010000000
VERSION     DAZFKY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..584
                     /locus_tag="ACHKCL_002964"
                     /pseudo
     CDS             1..584
                     /locus_tag="ACHKCL_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            687..1190
                     /locus_tag="ACHKCL_002965"
     CDS             687..1190
                     /locus_tag="ACHKCL_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5763889.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1257..2714)
                     /gene="nuoN"
                     /locus_tag="ACHKCL_002966"
     CDS             complement(1257..2714)
                     /gene="nuoN"
                     /locus_tag="ACHKCL_002966"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5763890.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2721..4250)
                     /gene="nuoM"
                     /locus_tag="ACHKCL_002967"
     CDS             complement(2721..4250)
                     /gene="nuoM"
                     /locus_tag="ACHKCL_002967"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5763891.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4481..6322)
                     /gene="nuoL"
                     /locus_tag="ACHKCL_002968"
     CDS             complement(4481..6322)
                     /gene="nuoL"
                     /locus_tag="ACHKCL_002968"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5763892.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6319..6621)
                     /gene="nuoK"
                     /locus_tag="ACHKCL_002969"
     CDS             complement(6319..6621)
                     /gene="nuoK"
                     /locus_tag="ACHKCL_002969"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5763893.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6618..7172)
                     /gene="nuoJ"
                     /locus_tag="ACHKCL_002970"
     CDS             complement(6618..7172)
                     /gene="nuoJ"
                     /locus_tag="ACHKCL_002970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5763894.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7184..7726)
                     /gene="nuoI"
                     /locus_tag="ACHKCL_002971"
     CDS             complement(7184..7726)
                     /gene="nuoI"
                     /locus_tag="ACHKCL_002971"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5763895.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7741..8718)
                     /gene="nuoH"
                     /locus_tag="ACHKCL_002972"
     CDS             complement(7741..8718)
                     /gene="nuoH"
                     /locus_tag="ACHKCL_002972"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5763896.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8715..11441)
                     /gene="nuoG"
                     /locus_tag="ACHKCL_002973"
     CDS             complement(8715..11441)
                     /gene="nuoG"
                     /locus_tag="ACHKCL_002973"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5763897.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11494..12831)
                     /gene="nuoF"
                     /locus_tag="ACHKCL_002974"
     CDS             complement(11494..12831)
                     /gene="nuoF"
                     /locus_tag="ACHKCL_002974"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5763898.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12828..13328)
                     /gene="nuoE"
                     /locus_tag="ACHKCL_002975"
     CDS             complement(12828..13328)
                     /gene="nuoE"
                     /locus_tag="ACHKCL_002975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5763899.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13331..15133)
                     /gene="nuoC"
                     /locus_tag="ACHKCL_002976"
     CDS             complement(13331..15133)
                     /gene="nuoC"
                     /locus_tag="ACHKCL_002976"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5763900.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15227..15889)
                     /gene="nuoB"
                     /locus_tag="ACHKCL_002977"
     CDS             complement(15227..15889)
                     /gene="nuoB"
                     /locus_tag="ACHKCL_002977"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5763901.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15905..16348)
                     /gene="nuoA"
                     /locus_tag="ACHKCL_002978"
     CDS             complement(15905..16348)
                     /gene="nuoA"
                     /locus_tag="ACHKCL_002978"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5763902.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4066 a         4685 c         4331 g         3434 t
ORIGIN      
        1 tttaccaacc ctgggtcact gcattatcgc caacgccccg acacgtaatt cctgagcaat
       61 tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc gacaatattg
      121 ataatctgaa taggtccgta gagtatataa aaaaaggtct ttgtcagtag cggtgccaga
      181 gttaccgcgc agggcgtccc caattggcta ttacgactct gcgcctggta gccaggatta
      241 cccttacccg caatgtcctg gctttatccc gatcgggggg actttattgc ctggtcggca
      301 gaatacagga tatcaacgcc gttcgtcagg taaaaacggc attgttatca tcccgggatt
      361 tatctgttta ttctatgaat gccccatggt ttattcacgg tattgatttc tctgaccacc
      421 ttaattattg gcaacacgat attctcgccg taatgattac tgacaccgct ttttatcgta
      481 ataaacaata ccacttgccc ggtgatacag cagacagatt gaattatcag aaaatggctc
      541 agatggtgga tggtgttata actttgttac acaacagtaa ataataaaaa tttctcgagg
      601 cgggtccaga aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa
      661 cataaaattc aatggaggaa aaataaatgg gaatgattgg ttattttgca gaaattgact
      721 cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg
      781 acacgctttc aggattacgc cgactggata ttgataaacg ctgggatttt ttacactttg
      841 ggctgacagg cacctctgcg ttcgatccag cgaagaatga tccgctgagt cgtgccgtgc
      901 ttggtgaaca cagcctcgaa gatggtatag atggtttcct gggattgaca tggaatcagg
      961 agttagccgc gacaatcgac cgactggaat cactggatcg aagtgagctg cgtaaaaagt
     1021 tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt
     1081 tagaggggca actctttgct tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc
     1141 gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcagg
     1201 gcaccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact gatagcttac
     1261 atcagcggca ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc
     1321 agtaccaaca gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac
     1381 ggtgcatcgc gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg
     1441 tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaggtgt
     1501 gcctggacac caaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata
     1561 ccggccagag acagcatcat caccgtcatc accgccgcga ggatcggacg atgccagaac
     1621 agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg
     1681 ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc
     1741 gcttccatcg acatctcgcc agtttgcagc gcaatcagcg ccaccagcag atagccgagg
     1801 tgagagatag atgagtaacc gagcagacgt ttgatattgg tctggctcag cgccatcagg
     1861 ttaccgaaga tgatggaggc aaaggcgata atcgccagca ccacccgaat cgcttcgctg
     1921 tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc
     1981 gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac
     2041 aggtggaacg gcaccagaga gagtttgaag ccgaggccaa caatcatcag gccgaaacct
     2101 gccagcaaca gcggctcgtt tagcataccg tcgccgaggt ttttacccag cgcgacaaac
     2161 gacaggtcgc cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct
     2221 gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg
     2281 taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc
     2341 agatggtttg cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag
     2401 aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg
     2461 aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc
     2521 ggcgtaacgt ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta
     2581 agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc
     2641 atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt
     2701 ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc
     2761 tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc
     2821 agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc
     2881 agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg taacatcgcc
     2941 agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca
     3001 acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt gcctggcatc
     3061 ccgagcgttg ccaccgcaaa gaacagtgac aaggctggca gccatttcat cttgctccac
     3121 agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accacaaaga
     3181 ataaacagac ccgccgctga caagccgtgc gcaatcatct ggattaccgc gccctggtag
     3241 gccaactggc tgccggtgta gatagcaatc agtacgaagc ccatgtggga aaccgaggtg
     3301 taggcgatca gacgtttgat atcggtctgg gcgaaggcca tccacgcacc gtagaagatg
     3361 ccgataacac ccagccacat ggcgattggc gcgaactctg ccgacgcgtt tgggaacagc
     3421 ggtagggaga aacgcagcag accgtaagcg gcggttttca gcaagatccc cgcgaggtcc
     3481 acggaaccgg cggtcggtgc ctgtgagtgc gcatccggca gccagccatg cagcggaacc
     3541 accggcattt tcaccgcaaa cgcgatgaag aagcccagca tcaacagata ttccacacca
     3601 ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg
     3661 tagtgaacaa aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg
     3721 taaatgaaga acttggttgc cgccgtgata cgcgttttac catcagaggc tttatgcccc
     3781 cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg
     3841 aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg
     3901 tggaagaagc cctgatattt ttcgatctct ttccacgaac agagtaccgc cagcacaccg
     3961 agcagaccgg tcagcacgac catcagcagc gacagcccgt caatggcgag atggatagag
     4021 ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg
     4081 gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat
     4141 cccatggtga tcagcgcgat ccagcgcggc accttgacgc caaagcgttc ggtctgccag
     4201 cacaggaagc cgccgataaa ggggattagt attaaccagg gtaataacat agctaagtat
     4261 gtcccttata tttactcctg ctcaggctat ctcactcgcc agcccgaatc ccggcagtgc
     4321 tcgcaatcct cacgtactta agtacgctcc ggttgctgtg cgctggcggt atccggtctg
     4381 acttcgccga tgacgcctgt acttcaggaa cagttaccta aaatttattc ttagttcaca
     4441 gacccggcgc aacgccgggt atattcactc agccaacaac tcaacgcagt accatcaaca
     4501 gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc agataaccgt
     4561 tctcgcttaa cagcaggcct ttacctgcaa agcgggaaag gacagccggg atgttcatca
     4621 ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc ttgacgaaca
     4681 ctttgtcgta cagccagtca aagccccagg cgttgtacca ccaggtgccc agcagacggc
     4741 ccggcgcact gttggcgatg gaggtcacca gagtacgttt acccagccac agccaggctg
     4801 ccagcagaat gccgaccacc gcgaccacgc cagaggtaat ttccagggtc aacatgctgc
     4861 cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca atcagtgcgc
     4921 caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt acccctttca
     4981 cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata cggaaggtgt
     5041 agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata tgaccattcg
     5101 ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgact agcggcagtg
     5161 ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata gatttacgca
     5221 gaccgcccat cttgaagatg ttctgttcgt gatggcaggc gagaatgacg gaaccggatg
     5281 ccaggaacag cagcgcttta aagaacgcgt gggtcatcaa gtgaaaaatc gccgcatccc
     5341 atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta gagtaagcga
     5401 gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg
     5461 ccccaacaat gcccaccaga tgcagaactt ccggcgtcat caggaacagg ccgtgggtac
     5521 gggcgatcag gtagacaccc gcggttacca tcgttgcggc gtggatcagc gcggagacag
     5581 gcgtcgggcc cgccatcgcg tcggcaagcc atgtctgcaa cggcaactgc gcagatttac
     5641 cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg ccgtcagcaa
     5701 agtgcgctgg tgccagttcc accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa
     5761 gaatgaacag cgcgaaagcg aggaacacgt cacccacacg ggtcacgacg aacgctttca
     5821 ttgccgctgc gccattcttc ggatcggtgt aatagaaccc gatcagcaga taggagcaca
     5881 ggcccacgcc ttcccagccg aggtacatca gcagcaggtt gtcggcaagc accagaacca
     5941 ccatgctggc gataaacagg ttggtgtaag cgaagaagcg agagtagccc tcttcaccgc
     6001 gcatatacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc accgagagca
     6061 tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg cctaccgaca
     6121 tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaag tcaacgccaa
     6181 taaaggcagt taccagcgcc gccaggccca cagagccaac gccgacgatc gccgagacgt
     6241 tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa
     6301 tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt tcaggttctg
     6361 gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg ccgccgcgag
     6421 gctgatggcg agaatgtaca tcacctgacc gtcggtctgg ccccagtaac ttccggcgac
     6481 cacaaaggcc agcgcggaag cgttaatcat gatttccaga ccaatcagca taaacagcag
     6541 attgcgacgg ataaccagac cggttaagcc aagaacgaat aagattgccg cgaggatcag
     6601 tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta
     6661 cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc cacaaccaga
     6721 cccgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc gaacagcgta
     6781 atacccactg ctttagcact gattggcgta ccgtcgatac cttgatcgtt aacaccgaga
     6841 atggcgtaaa caatcaccac caacatgatg gctgacaaaa ttgccggacc aatccacacc
     6901 tgcggtttca gccactggcg ttcttgttcg atttctgaac cgcccaggtt gagcatcatc
     6961 accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc cagcgcaccg
     7021 gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg ccagcagcga aataatcagg
     7081 tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc aagtatggct
     7141 atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt aacaggctct
     7201 tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg tcgattgcca
     7261 tacctgccat ccggtagaag ttatactccg ggtatttgcc cggaccggag atcagcagat
     7321 cctctttctc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa tccggggtta
     7381 actgaatcgc cgtggtcgga caggcttctt cgcacaaacc acagaaaatg cagcgtgaga
     7441 agttgatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct ttttgcagcg
     7501 agatacagcc gaccgggcag gctaccgcgc agaggttaca ggctacgcaa cgctcttcgc
     7561 cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga tagaccggct
     7621 cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc atccagatac
     7681 tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc ttattgcccc
     7741 ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca gcgtcagcgg
     7801 caggcagatt ttccagccga aggacattac ctggtcataa cgcggacgcg gtaacgacgc
     7861 acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc agatgaatgg
     7921 cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg cagagatggt
     7981 cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg aatattcaat
     8041 gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt gacggtgaca
     8101 caccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga tgacgttcca
     8161 cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct gcgccaccac
     8221 gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag aagcacgcat
     8281 cgcaccgagc agcgagtatt tgttgttact tgaccagccc gcaaacagca ccgcgtaaac
     8341 cgccagaccg gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca
     8401 acccggactg actggcacaa tcgcaaaggc cagcagcagc gaggtaaagg caatcatcgg
     8461 tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt taaagaacat
     8521 tttgatcatg tccgcaacca gctggagcga accaccccag ccaacacggt taggtccgta
     8581 acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg ccccgcaggt
     8641 gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct caatcagttc
     8701 cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc gccagccagc
     8761 accggagcaa tgccggacat acccatcggc aagcccacct gccctgccgt cagcccttca
     8821 gcgatttcaa ccggcagagt gaccgtgttg ccatcgtaac taaaggagac gcgtgtacct
     8881 gcgtttacac ccaacttcgc ggcatccgct gggttgagtt tgatgtacgg ctgcggcata
     8941 cgacactgga agaccggagc acgctgtgac aattcatcgc tgccaaacag gtggtagtac
     9001 ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa
     9061 tccagaccat tttcgctggt ttcaaacaga cgcacacccg gatcgccaaa gcgcagtttg
     9121 ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc cggagcccag
     9181 gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt taccttccat cgagaaggtg
     9241 aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg
     9301 gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg aatacggaat
     9361 gtcgcatctg gcgcagcatc tttgataccc gccagttccg ggattttcgc cacaacagcg
     9421 tcaattacat ggtcgagctg cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc
     9481 gagtgcagcc agcgccaact ttccagcatg acagttttgc tgtcgtaata agccggatcg
     9541 taaacctgga agaaacgttg ggcgcgacct tcattattaa ttaccgtacc gtcgctttca
     9601 gcaaagctgg cagcggaaag caccagatgg gcgttttcca tgatcgctgt gcgttgatgg
     9661 tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt agcagaagcg
     9721 tgacgatgca gatcgttttc cagcaccacc accgcgtcgg cgcgtccggt ttccagttcg
     9781 gttaacgctt cttcaagcga tccaccgccc ataatgccca accccatgct gttgacggaa
     9841 cgggcaatca tggtgatacc gacgtcagcg ccgcgacctt tcagggcttt cgcgacgtta
     9901 gccgcagcct gaatcacctc taagctaccg gcgttcgtcc cggagataat caacggtttc
     9961 ttcgcacccg ccagtgcctg cacgatgacg tcgattttgc tttgcagctc aggttcaata
    10021 ccgtcaaccg ctggcgcaga gttatccagc gcatgggcga tggcaaaacc taaacgcgcc
    10081 tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg ggtgtcatca
    10141 acgttggtaa caaacagcgg atgcttagca cgttgaccga tattgaggat tgccgcaatc
    10201 tgccagtcag ccactttctg tgctgccgcc atttcgcgcg ctttaccttt caccgcctgg
    10261 cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag caccagtacc
    10321 gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc acgcagcact
    10381 ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta gaagttttct
    10441 tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg agaaccaata
    10501 ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc ctgttcggcg
    10561 ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg atctttcagg
    10621 ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt taccgtaccg
    10681 ttgtaacggt tttcgatacg acgcagttcg ccataacgct cgccggggct gatgttacag
    10741 ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt acggttgtaa
    10801 cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac caggttaccg
    10861 gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt gtcatgcgcg
    10921 ccataaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac gcagcggtag
    10981 caggcgatgc agcggttcat ttcgtgagag atgaacggcc ccaaatcctg attacggtgg
    11041 gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacggt catatcctga
    11101 agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac
    11161 cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga aataaaggtg
    11221 ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc ttccgcgttt
    11281 tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag
    11341 caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt gtccgctccg
    11401 ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct tccagttttc
    11461 tcagtcagag actgagcgtt aatcgaaatt cggttaccag cgctctttca gcaggttcgg
    11521 ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgatccccg cctcaaattc
    11581 ttcgcggaaa tatttgatgg cgctctgtaa cggctccaca gcaccaggtg cgtgggcaca
    11641 gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga tatcgcccgg
    11701 ctgaccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca gaccgtcgcg
    11761 gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt tacgcaccag
    11821 cgacaccatg ttgatttcat ggtcaaccgc catcgccagc gccgtaccca gacggctgcc
    11881 cgctttaccg atactttcga attccatcgg caggtcaagg tgcgcttcgg tcaggaagtc
    11941 ggtgcccgcg ccgcctggct gccaggcttt gaatttcaga ccgtcacgca taccaccggc
    12001 gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca gtcccggatt
    12061 tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt tcgagatgtt
    12121 ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg tttcgacgtt
    12181 gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg gtttcgagcg
    12241 tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc cgcagatgta
    12301 gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca taatgttttt
    12361 gccaagcaat cccgcttcag tcgcttcggc aatggcgcgg cgcagattta ctgccgcttc
    12421 aatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa acgcggagat
    12481 gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt ctttataggt
    12541 gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg attcgtcttt
    12601 cggcatcagg ctccacttca agccagtcga aaagcccgcg ccgccgcgcc ctttcagacc
    12661 agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc
    12721 cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt tgtcatcgcg
    12781 cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca tttataccgc
    12841 tccagcagtt cagggatcgc ttccggggtc agattcgcgt gagtgtcctc atcgatcatc
    12901 atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt aaagcggcca
    12961 tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc ctgaataccc
    13021 tgatacccat tgatatgaca gaccacgctg tcgcaataac ggatcacatg gcggccaacc
    13081 ggctggcgga agatctggct gtagaacgtt gccacgcctt cgacgtcgct tgccggaata
    13141 cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg ctgcttctga
    13201 acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc
    13261 tcgatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc
    13321 tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc agataaacaa
    13381 tcaggtcaga caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac
    13441 tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc aggtagtaac
    13501 tgttgatccc tttggtcgcc tcaatcatct ggaaggattc attggcaggc atcaccggcc
    13561 cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc gtgcgctctt
    13621 tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc atgttgttga
    13681 ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttaagc attacgcggg
    13741 tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt tcatagccag
    13801 aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg cccgcgccag
    13861 tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg gaacgacctt
    13921 tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc ggcatccagt
    13981 cgaggaactc acgcagcagg cgatcccagc cgcgcggcag gtcgtgcgct acgccgccaa
    14041 tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga tcgtaaattt
    14101 tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc tgaataaagg
    14161 tcgagatata cagcaggtgg ctgttgatgc ggaacagttc ggagagcatt acgcgaatga
    14221 cgttaacgcg agccggcacg gtgatcccgg ccagtttctc taccgccagc acgtaaggca
    14281 tttcgttaac gcagccgccg aggtattcga tacggtcggt atacggaatg tagctgtgcc
    14341 aggactggcg ttcgcccatt ttctccgcac cacggtggtg gtagccgatg tctggtacgc
    14401 agtcgacaat ctcttcgccg tcgagctgca aaacgatacg gaaagcaccg tgcgccgacg
    14461 ggtggttcgg accgaggttg aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc
    14521 cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg gctttggtca
    14581 gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc tttacgcagc gggtgacctt
    14641 tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaat gtaatgccaa
    14701 acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg
    14761 gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga
    14821 tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg cggtgcgtgc
    14881 gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct
    14941 ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg ggaaccccgg
    15001 tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt tcgccaatta
    15061 ccggatcatc aagatgatcg cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca
    15121 tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagcaagct tcgcgaaata
    15181 gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa atctcgtcag
    15241 gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca
    15301 tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga cgttctttgc
    15361 cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc ggcgggcagc
    15421 ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg gaataaatat
    15481 cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt ggttccagca
    15541 tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt cctgcaacca
    15601 ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa cgcgccacgt
    15661 catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga ccgaagttat
    15721 acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg cccataaaca
    15781 cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt tgcagggggt
    15841 aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc ttaatgcctc
    15901 gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg
    15961 aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga cctgccagta
    16021 acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg cggatagagg
    16081 ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac atggccacca
    16141 gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata ccggattcaa
    16201 acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca
    16261 tcaggcaaca taggccaatg gcaacgataa gaaagatagc gaatgcccag tgatgagcga
    16321 tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg ccagattcac
    16381 tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa aaaaccttac
    16441 aatcactgta gaaattcttt tatacagcta attgatgtgg tcttttactc ctttctataa
    16501 ccttttgtca acttta
//
LOCUS       DAZFKY010000104        15964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFKY010000104 DAZFKY010000000
VERSION     DAZFKY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            207..1550
                     /locus_tag="ACHKCL_002979"
     CDS             207..1550
                     /locus_tag="ACHKCL_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763903.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1699..3207)
                     /gene="putP"
                     /locus_tag="ACHKCL_002980"
     CDS             complement(1699..3207)
                     /gene="putP"
                     /locus_tag="ACHKCL_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5763904.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3366..3575)
                     /locus_tag="ACHKCL_002981"
     CDS             complement(3366..3575)
                     /locus_tag="ACHKCL_002981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763905.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3630..7592
                     /gene="putA"
                     /locus_tag="ACHKCL_002982"
     CDS             3630..7592
                     /gene="putA"
                     /locus_tag="ACHKCL_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5763906.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7632..8270)
                     /gene="rutR"
                     /locus_tag="ACHKCL_002983"
     CDS             complement(7632..8270)
                     /gene="rutR"
                     /locus_tag="ACHKCL_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5763907.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8558..9649
                     /gene="rutA"
                     /locus_tag="ACHKCL_002984"
     CDS             8558..9649
                     /gene="rutA"
                     /locus_tag="ACHKCL_002984"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5763908.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9649..10341
                     /gene="rutB"
                     /locus_tag="ACHKCL_002985"
     CDS             9649..10341
                     /gene="rutB"
                     /locus_tag="ACHKCL_002985"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5763909.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10353..10739
                     /gene="rutC"
                     /locus_tag="ACHKCL_002986"
     CDS             10353..10739
                     /gene="rutC"
                     /locus_tag="ACHKCL_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5763910.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10747..11547
                     /gene="rutD"
                     /locus_tag="ACHKCL_002987"
     CDS             10747..11547
                     /gene="rutD"
                     /locus_tag="ACHKCL_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5763911.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11557..12147
                     /gene="rutE"
                     /locus_tag="ACHKCL_002988"
     CDS             11557..12147
                     /gene="rutE"
                     /locus_tag="ACHKCL_002988"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5763912.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12158..12652
                     /gene="rutF"
                     /locus_tag="ACHKCL_002989"
     CDS             12158..12652
                     /gene="rutF"
                     /locus_tag="ACHKCL_002989"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5763913.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12673..14001
                     /gene="rutG"
                     /locus_tag="ACHKCL_002990"
     CDS             12673..14001
                     /gene="rutG"
                     /locus_tag="ACHKCL_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5763914.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14084..14191)
                     /locus_tag="ACHKCL_002991"
     CDS             complement(14084..14191)
                     /locus_tag="ACHKCL_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFO5763915.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            15181..15810
                     /locus_tag="ACHKCL_002992"
     CDS             15181..15810
                     /locus_tag="ACHKCL_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO5763916.1"
                     /translation="MTSQRIPVFNGTIANETTLLVNARDLHTFLGVGKRFASWITERI
                     EEYGFVENQDYIAISQKREIGYGRGKKDYHLTLDTAKETAMVERNEKGRQIRRYFIEC
                     EKKLRNMQSAQAEPQQQFTDEEIILLCYMQLWMEKAQDLSKHLYPIMKELNSSYTNKL
                     YDIAFETIYMVTKNRDVLLREAARLDQSSFVVQRARPMLKSLRARQFEF"
     gene            15858..>15964
                     /locus_tag="ACHKCL_002993"
     CDS             15858..>15964
                     /locus_tag="ACHKCL_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5763917.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHC"
BASE COUNT         3867 a         4255 c         4184 g         3658 t
ORIGIN      
        1 ggcagcattg taaattcgcc aataactggc attcctcctg ctttttacgg gtgtttccat
       61 taacaccaga atgagcggat tgtttactac atcaagaacg catccgaagt gtacatcctg
      121 gtgcgccgac tgaatcgccc ggagccatgc ggatggctac gacttaatac gaatcattaa
      181 ccctgtaagg atacggagag cattacatga gaccaatatc caacctcaat acgagcgcag
      241 caacctatat tccaccgcaa caactcccga gtagttgtct ggaaactttg acgcttctgg
      301 tgcagatgaa taattatatc aaaagggatg cggactacag cacgggcatg gctgtagctc
      361 cactggttcc tgaagaggtt cacttgctgg ctgctgcgat gacaaccgaa ctgcatcgac
      421 atcagttcca accggtggta agtgcaaatg atctgcaact tcctgaaccg ttcaccttcg
      481 atgttgccgg tttcaccatc accttcacga agacgcaaga gcatgacaac acgggcaatc
      541 tcatgaaaat tgcagtcagc aagagcggtg tgtgcaccag tacaaatatt actcttgagc
      601 tgtttcactc cattgtcaca accctgatgt cacgcagtca atatggaacc tttgatctct
      661 ggtccgtacg gccaatactc acggatgaaa gtcaaaacag ggtgcatgaa gcagctcgct
      721 actccccggc ccagcaatac ggaagagaag aaacacactt taccaacaat ggcatgcgag
      781 aatttggaaa tatgtctccg ctggcgtggc gtaacgatgt ggaattacag caatcgtgta
      841 atacaagcaa catcccacca aatgctgcaa atgacgacat aaacaacacc aggcagacca
      901 tcgaggacca gccagaggcg gacgagccac agcaatatgt taaattgact gtagacgaca
      961 tgcgaaaatg ggcggctatg gaccagcagg cgcgaaacgc tctacaggga gtttcaggat
     1021 ggtgtacgcg caatcatttt aatatcaaaa atgccagaaa ttacctgacc gatcacggcc
     1081 tgaactacgc gggacaggta aaggtaaaca ggccgcatga atatgccaaa ttcacccttg
     1141 aacatatccg ccagtgggca gccttaaaca agcatgtgcg aaaaccggtt ggttatttag
     1201 aaaagtggtg taaggaaaga aacctcgcgc caactactgc caggaattac ctgaagaacg
     1261 acgggcttac cgccctgggt gagttgaagc tgacagggcc gcagaaatgg gtcacgttca
     1321 ccttcggaga catcgtacaa tgggcgaata tgacccagga ggagcgtaac agcgcagggg
     1381 gagccgaaaa gtggagtaaa aaacgcggct tccagtggtc tactgccaga tcctacctga
     1441 aatcgagcgg agtgaccaag caaggtgcga gaaaactggc gtggttaaaa aacagcggga
     1501 atatgtcgaa tccattctat ctcgcccaac caaccagccg caggacataa gctgaaggta
     1561 ccgatagcgg caattttgtt catctgtgcc gggtgggcaa atagcaattg cccccgaaca
     1621 ctccctgcca ggcaacttat accgcatggc agggaacatt cgggttaaaa agcaaaagat
     1681 aactaattac tcttcagctt agccttcctg caaccgtgac ggcggagccg aatgatagtg
     1741 cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac ggtgctttac ccagcaaact
     1801 aaacactaca atcccaatac tgccaaagat aaagcccgga ataatttcgt acagtcccag
     1861 ccagccgaac tgtttccaga cgatcaccgt cagcgcaccg atgatcatcc ccgccagcgc
     1921 accgttgcgc gtcatgcgtg accacatcac cgaaaacagc accaccggac caaacgccgc
     1981 gccaaagcct gcccacgcgt agctcactaa gcccagcacg cggttttccg ggttcgctgc
     2041 caacgcaatc gccaccagcg ccaccaccag caccatcaca cgccctaccc acaccagttc
     2101 tttctggctg gcatgtttac gcagaaacgc tttgtacaaa tcttcggtaa tcgcactgga
     2161 acataccagc agctggcaac ttaacgttga cattaccgcc gccagaatcg ccgacagcag
     2221 aatcccggca atccacgggt taaacagaat ttgcgccagt tcgataaaca cgcgctcggc
     2281 gttctggttt accgcaccag ctaccgacgg gtgctcgtta aagtaagcga taccaaagaa
     2341 gccgacagcc accgcccctg ccaggcagag gatcatccag gtcatactga tacgacgcgc
     2401 atggacgatg ctgtggtgag aatccgccgc cataaaacgc gccaggatgt gcggttgccc
     2461 gaagtaaccc agcccccacc ccatcagcga gataatggcg acaaagttca gccctttgag
     2521 catatccacg ttttcgatgc tcttttgttt gatcacttcc agcgagtcac caaagccacc
     2581 gacactgata atgacgataa ccggcgtcag gatcagggca aaaatcatca ggctggcctg
     2641 tacagtgtca gtccagctca ccgcgaggaa accgccaata aaggtgtaaa ggatcgtcgc
     2701 cgcagctccc gcccacagag ccgtttcgta gctcatgcca aaggtacttt caaacagacg
     2761 cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag aacagcaaaa tcaccagcgc
     2821 ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc ccggtgaaat aatccggtaa
     2881 tgttaaggcg ttattgttgt attcggtatg cacacgcaac cgcccggcca ccagcttcca
     2941 gttaatccac gcgcctaatg tcaggccaat ggcgatccaa ctttcggaaa tcccggaaag
     3001 aaaaaccgcg cccggcaacc ccattaacag ccagccgctc atatccgatg cacccgccga
     3061 taatgccgtc acgaatggcc caaggctacg tccgcccaga atatagtcgt caaagttttt
     3121 cgttgatcgc caggcgataa acccaatcaa tatcatgcca aagatataga cacaaaatgt
     3181 caccaacatc ggtgtgctaa tagccatcta aagtctccaa aaaattatta tcggcaatgt
     3241 cgaaacttgc cgttatatct gccaccggaa cggggtaaca gagtttatgt tttaccaggg
     3301 cgaccgtatc ctgccggaag cgctggttat tcacaatcga tttaacacac catttacatt
     3361 aaattttagt gctcagcgac actatttttc atcaggttgc actctctcac attttttgcg
     3421 gttgcacctt tcaaaaatgt taactgccgc agagaaaaag tctgagttat ttttttaatc
     3481 cctgtcatat cgatttcttt tattaacatt tcattcattt ttaagcttgc tacgcaggtc
     3541 acatttaaca tggttgcaca aagttgcaac atcatggata tttcacgata acgttaagtt
     3601 gcacctttca gaacaacagg agtaatggca tgggaaccac caccatgggg gttaagctgg
     3661 acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc acgccacact
     3721 ggttaattaa gcaggcgatt ttttcttatc tcgaacaact ggaaaacagc gatactctgc
     3781 cagagctacc tgcgctgctt tctggcgctg ccaatgagag cgatgaagca ccgactccgg
     3841 cagaggaacc acaccagcca ttcctcgact ttgccgagca aattttgccc cagtcggttt
     3901 cccgcgctgc gatcaccgcg gcctatcgcc gcccggaaac cgaagcggtt tctatgctgc
     3961 tggaacaagc ccgcctgccg caaccagttg ctgaacaggc acacaaactg gcgtatcaac
     4021 tggccgataa actgcgtaat caaaaaaatg ccagtggccg cgcaggtatg gtccaggggt
     4081 tattgcagga gttttcgctg tcatcgcagg aaggcgtggc gctgatgtgt ctggcagaag
     4141 cgttgttgcg tattcccgac aaagccaccc gtgatgcgtt aattcgcgac aaaatcagca
     4201 acggtaactg gcagtcacac attggtcgta gcccgtcact gtttgttaat gccgctacct
     4261 gggggctgtt gtttactggc aaactggttt ccacccataa cgaagccagc ctctcccgct
     4321 cgctgaaccg cattatcggt aagagcggtg aaccgctgat ccgcaaaggt gtggatatgg
     4381 caatgcgcct gatgggcgag cagttcgtca cgggcgaaac catcgcggaa gcgttagcca
     4441 atgcccgcaa gctggaagag aaaggtttcc gttactctta cgatatgctc ggcgaagctg
     4501 cactgactgc cgcagacgca caggcgtaca tggtttccta tcagcaggcg attcacgcca
     4561 ttggtaaagc gtctaacggt cgtggcatct atgaaggtcc gggcatttca atcaagctgt
     4621 cggcgctgca tccgcgttac agccgcgccc agtatgaccg ggtaatggaa gagctttacc
     4681 cgcgtctgaa atcactcacc ctgctggcgc gtcagtacga tattggtatc aacattgacg
     4741 ccgaagaggc cgatcgtctg gagatctccc tcgatctgct ggaaaaactc tgtttcgagc
     4801 cagagctggc aggctggaac ggcatcggtt ttgttattca ggcttatcaa aaacgctgcc
     4861 cgctggtgat tgattacctg attgatctcg ccacccgcag ccgtcgccgt cttatgattc
     4921 gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg cgcgcagatg gacggccttg
     4981 aaggttatcc ggtttatacc cgcaaggtat ataccgacgt ttcttatctc gcctgtgcga
     5041 aaaagctgct ggcggtgccg aatctaatct acccgcagtt cgcaacgcac aacgcccaca
     5101 cgctggcggc gatttatcaa ctggcaggtc agaactacta tccgggtcag tacgagttcc
     5161 agtgcctgca tggtatgggc gagccgctgt atgagcaggt caccgggaaa gttgccgacg
     5221 gcaaacttaa ccgtccgtgt cgtatttatg ccccggtcgg cacacatgaa acgctgctgg
     5281 cgtatctggt gcgccgcctg ctggaaaacg gtgctaacac ctcgtttgtt aaccgtattg
     5341 ccgatacctc tttgccactg gatgaactgg tcgccgatcc ggtcactgct gtagaaaaac
     5401 tggcacaaca ggaagggcaa actggattac cgcatccgaa aattcccctg ccgcgcgatc
     5461 tttacggtca cgggcgcgac aactcggcag ggctggatct cgctaacgaa caccgtctgg
     5521 catccctctc ttctgccctg ctcaatagtg cactgcaaaa atggcaggcc ttgccaatgc
     5581 tggaacaacc ggtagcggca ggtgagatgt cgcccgttat taacccggcg gaaccgaaag
     5641 atattgtggg ctatgtacgt gaagccacgc cgcgtgaagt agagcaggcg ctggaaagtg
     5701 cggttaataa cgcgccaatc tggtttgcca ctcctccggc tgaacgcgcg gcgattttgc
     5761 atcgcgctgc cgtgctgatg gaaagccaga tgcagcaact gattggtatt ctggtgcgtg
     5821 aagccggaaa aaccttcagt aacgccattg ccgaagtgcg tgaagcggtc gattttctcc
     5881 actactacgc cggacaggtg cgggatgatt tcgctaacga aacccatcgt ccattagggc
     5941 ctgtggtatg tatcagcccg tggaacttcc cgctggcgat tttcaccggg cagatcgctg
     6001 ccgcactggc ggcaggtaac agcgtactgg caaaaccggc agaacaaacg ccactgattg
     6061 ccgcgcaagg gatcgccatt ttgctggaag cgggcgtacc gccaggcgtg gtgcaattgc
     6121 tgccaggtcg gggtgaaacc gtgggcgcgc aactgacggg tgatgatcgc gtacgcgggg
     6181 tgatgtttac cggttcaacc gaagtcgcta cgttactgca gcgcaatatc gccagccgcc
     6241 tcgacgctca gggtcgccct attccgctca tcgctgaaac cggcggcatg aacgcgatga
     6301 ttgtcgattc ttcagcactg accgaacaag tcgtaataga tgtgctggcc tcggcgttcg
     6361 acagtgcggg tcagcgttgt tcggcgctgc gcgtgctgtg cctgcaagat gagattgccg
     6421 accacacgtt gaaaatgctg cgcggcgcaa tggccgaatg ccggatgggt aatccgggtc
     6481 gcctgaccac cgatatcggt ccggtgattg atagcgaagc gaaagccaat attgagcgcc
     6541 atattcagac catgcgtagc aaaggccgtc cggtgttcca ggcggtgcgg gaaaacagcg
     6601 aagatgcccg tgaatggcaa agcggcacct ttgtcgcccc gacgctgatc gaactggatg
     6661 actttgccga attgcaaaaa gaggtctttg gtccggtgct gcatgtggtg cgttataacc
     6721 gtaatcagct gccggagctg atcgagcaga ttaacgcttc cggctatggt ctgacgcttg
     6781 gcgttcatac gcgtattgat gaaaccatcg cccaggtcac tggctcagcc catgttggta
     6841 acctgtatgt taaccgtaat atggtgggcg cagtggttgg ggtgcagcca ttcggcggcg
     6901 aagggttgtc cggtaccggg ccgaaagcag gcggtccgct ctatctctac cgtctgctgg
     6961 cgaatcgccc ggaaagtgcg ctggcggtga cactcgcgcg tcaggatgca gagtatccgg
     7021 tcgatgcgca gttgaaagcc gcattgactc agccgctaaa tgcactgcgg gaatgggcag
     7081 caaatcgtcc agaattgcag gcgttatgta cgcaatatgg cgagctggcg caggcaggaa
     7141 cacaacgatt gctgccaggg ccgacgggtg agcgcaacac ctggacgctg ctgccgcgtg
     7201 agcgcgtatt gtgtatcgcg gatgatgaac aggatgcgct gactcagctc gccgccgtgc
     7261 tggcggtggg cagccaggtg ctgtggccag atgacgcgct gcatcgtcag ttagtgaagg
     7321 cattgccatc gactgtcagc gagcgtattc aactggcgaa agcggaaaat attaccgctc
     7381 aaccgtttga tgcggtgatc ttccacggtg attcggatca gcttcgcgca ttgtgtgaag
     7441 cagttgccgc gcgggatggc gcaattgttt cggtgcaggg ttttgcccgt ggcgaaagca
     7501 atatcctgct ggaacggctg tatatcgagc gttcgctgag tgtgaatacc gctgccgctg
     7561 gcggtaacgc cagcttaatg acgatcggtt aaacaggtag ccggaggatg ttacaccctc
     7621 ctccggcatc attagcgtgg tcgaatcccc tcaataataa tccgctgcac gttttcaacc
     7681 gtttgattga aaaatacctc atcgcgcaac gttgcgcctg tcaccgcctc cacctgaggg
     7741 gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa aaatcaaatg ctgcggatca
     7801 atcggcgcga gtttgccgct tttgacccaa ccggcaatca gcgccgattt ctcatcaatt
     7861 aatgacttca aatcgcccgt cagttcatcc attaacagcg gcgcgcctgc cagcatctcc
     7921 atacagaaca ggcgcgaagc ctgcggataa tcgcgtgaga cttccagctt cagacggatg
     7981 tactctttga tcgccgccag cggggcgaaa tcttcacgaa acgcttttaa cggtgccagc
     8041 cagatatcga gaatctgccg cagcacggca atatacagcg cctcttttga cggaaagtaa
     8101 tacagcagat tggtttttga aacacccgcc aactctgcaa tttgctccag ccgtgtgccg
     8161 tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt tttcttcgcg
     8221 cttactgcgc gcgaacgttt acccgttgtt ttcactgcgc cttgcgtcat gcgctctccc
     8281 ctcttgtaga ttggcaacag gatagcaaaa cccccgcccg gcctcgacct tctgcactct
     8341 catcgcgcac agatgcatgt tttatgtgca actgttttga ccgtttagtc cactttttac
     8401 cagacatttt agataacgcg atgttaacac attaataaca ctcccttttt aaaaactggc
     8461 atccgctttg caaacaagcc aatacgcggc tgaaaaggat atgcagaatg cagcgccccg
     8521 tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc
     8581 tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc cgacctttga
     8641 actgaataaa gccatcgtgc aaaaagcgga gcactaccat ttcgatttcg ccctgtcgat
     8701 gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt
     8761 caccttgatg gcggggctgg cggccgtgac ctcgcgcatt cagatttacg ccaccgccgc
     8821 caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg actccatctc
     8881 tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgagt atgagcagat
     8941 gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca ccgagtatgt
     9001 tcaggtgctg cgcgacctgt ggggcacggg aaaaagcgat tttaaaggcg attttttcac
     9061 catgaatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga tctgcgccgg
     9121 gcaaagcgac gctggcatgg cgttctccgc tcagtatgcc gatttcaact tctgtttcgg
     9181 caaaggcgta aatacaccca cggctttcgc cccgaccgct gcgcggatga aacaggccgc
     9241 agagcaaacc gggcgcgacg ttggctctta tgtattgttt atggtgattg ccgatgaaac
     9301 cgacgatgcc gctcgcgcca aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt
     9361 aagctggcta accgaacaaa gtcagaaaga tacccgctcc ggtactgaca ccaacgtccg
     9421 tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta
     9481 cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct
     9541 gttaaccttc gacgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact
     9601 gatgcagtgc cgcgcccatc tccctgtgct gactcaggag gtggcatgat gacgacatta
     9661 accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat cgtggtggat
     9721 atgcaaaacg cctatgccac accaggcggc tacttagatc tcgccgggtt tgatgtctca
     9781 accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcgcg agcggcaggg
     9841 atgttgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggccct
     9901 ggctcaccga attttcataa atcgaacgcc ctcaaaacca tgcgtaaaca gccgcagctg
     9961 caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga actggtgccg
    10021 cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa tacgccgctg
    10081 gacagcattt tgcgcagccg cggaatacgc catctggttt tcaccagcat cgctaccaac
    10141 gtctgcgtcg aatcgacgct acgcgatggc ttttttctgg agtatttcgg cgtggtgctg
    10201 gaagacgcaa cacaccaggc ggggccggaa tttgtacaga aagccgcgtt gttcaatatc
    10261 gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttctcccacg
    10321 tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt attcccgctg
    10381 gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatggc gtggtgtatg
    10441 tctccggtac gctggctttt gatcaacaca ataacgtgct gtttgccgat gacccaaagg
    10501 cgcaaacccg ccacgttctg gaaactatcc gcaaggtaat cgagacagcg ggtggcacga
    10561 tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta
    10621 acgaaaccta cgccgagttt tttccaggtg ataaaccggc gcgattctgc attcagtgcg
    10681 gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag
    10741 gccgcgatga aactttcact ctcacctccc ccttatgctg atgcgcccgt agtggtgttg
    10801 atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt gctggagcag
    10861 gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga cacgctggca
    10921 gaagattaca gtatcaccca gatggcagcg gaactgcatc aggcgctggt agccgcaggg
    10981 attgagcatt acgcagtggt cggccatgcg ctcggtgcgc tggtgggaat gcagctggcg
    11041 ctggatcatc ccgcgtcggt aactgtgctg gtctgcgtta acggctggct acgaataaac
    11101 gcccatacgc gccgctgttt tcaggttcgc gaacgattac tgtatagcgg tggcgcgcag
    11161 gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc ggcccgcgca
    11221 cctcgccttg aggcagaaga cgcgctggca ctggcgcatt ttcagggcaa aaataattta
    11281 ctgcgtcgac ttaacgccct caaacgcgct gactttagtc accatgcgga tcgcatccgc
    11341 tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccatcagc atgttccagt
    11401 gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgcgctatgg cggacacgcc
    11461 tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct tgccagcctg
    11521 ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt tagcccaggt
    11581 gcgcttagca ccctgttcac cgatgcccgc actcacaacg gctggcggga gacacccgtc
    11641 agcgatgaga cgttacggga gctttatgcc ctgatgaaat gggggccgac atcagctaac
    11701 tgttctccgg cacgtatcgt gtttatccgc actgcagagg gaaaagaacg tctgcgcccg
    11761 gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc tatcgtcgca
    11821 tgggacagtg aattttatga acggttaccg caactgtttc ctcacggcga tgcccgcagt
    11881 tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag ttccatgcag
    11941 gcggcttatc tgatcatcgc ctgccgtgcg ctggggctgg ataccggccc gatgtcgggt
    12001 tttgatcgtc aacacgtaga cgacgctttt tttgcaggca gcacgctgaa gagcaatctg
    12061 ctgattaata tcggctatgg cgatagcagc aaactttttg cgcgcctgcc acgtctgtcc
    12121 tttgaagaag cctgcgggct gttgtaagga gcaaactatg aacattgtcg atcaacaaac
    12181 ttttcgcgat gcgatgtcct gcatgggcgc ggcggtcaat atcatcacca cggacggtcc
    12241 agccgggcgc gccgggttca ccgccagcgc cgtctgcagc gtgaccgaca cgcctcccac
    12301 attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac
    12361 gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt ttggcggcaa
    12421 aacgcccatg gaacaccgct ttgccgccgc ccgctggcag aatggtgtga ccggatgccc
    12481 gcaactggaa gaggcgctgg tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg
    12541 cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca ccccctacgg
    12601 gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt aacctcctta
    12661 aggagacagc tgatggcaat gttcggtctt cctcactggc agttaaaatc gacctctaca
    12721 gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc agtaatgggc
    12781 gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccgattct gatggggctg
    12841 gatcccaatc tttccatttt aatgtcaggg atcggcacgt tactgttttt ctttattacc
    12901 ggcgggcgcg tgcccagtta tctcggctcc agcgcggctt ttgtcggcgt ggtgatcgcc
    12961 gcaacgggct ttaacggtca gggcattaac ccgaatatca gcattgcgct gggcgggatt
    13021 atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat cggtacgcgc
    13081 tggattgaac ggctaatgcc gccggtggtg acgggtgcgg tggtgatggc gattggcctg
    13141 aacttagcgc ctatcgcggt aaaaagtgta tcggcttcag gctttgatag ctggatggcg
    13201 gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta cccgtggcat gatccagcgg
    13261 ttattgattc ttgtaggatt aattgtcgcc tgtctgctgt atggcgtgat gaccaacgtt
    13321 ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg gttcggtctg
    13381 ccacattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc gcccgtggcg
    13441 gtgattctgg tggcggaaaa cttaggtcat ctcaaggcgg tcgccgggat gaccggacgc
    13501 aatatggacc cgtatatggg gcgagcattc gtaggcgatg gtctggcaac gatgctttct
    13561 ggttctgtcg gcagtagcgg cgtcaccacc tatgctgaaa atatcggtgt gatggccgtg
    13621 acaaaagtct actcgacgct ggtgtttgtt gctgctgccg tcatcgccat gctgttgggc
    13681 ttttcaccga aatttggcgc gctgattcat accattccgg ccgcggtgat tggtggtgca
    13741 tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa ggatatgggt acaaaaccgt
    13801 gtcgatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt gctgggcgcg
    13861 ggtgattttg ctctcacgct gggcggtttt acgttgggag gaatcggtac agcaaccttt
    13921 ggcgcgattt tactcaatgc gttattaagc agaaggttgg ttgacgttcc accgccagaa
    13981 gttgttcatc aggagccata aacagaaaac cgccggggtt accggcggtg atggcttttt
    14041 gacgagctta agaaaaaaat gcattcgtca ttgattagcc cggctagttg tcgcttttgc
    14101 cgtgactgct cttgccacct tttttacctg cttctgatgc tctttcgcgg tcttctgcaa
    14161 aattgccgga accgcctcga tggtttgcca ttactgacct ccgtctgggt atagacatca
    14221 tattgctaga attaacgcgg cgtatggcgg ctgccatatt ttcgctacgt gggactaagc
    14281 ttagtgaact gacgtgtttc tacccgcgtt tggtaatatc ctgcaacagc ttcttagcga
    14341 gaatagatat catctccaat aattatccat aagccgctct tatgattaaa aaaacgcgat
    14401 gagataaaaa tgtgtcattt tgcgacaaaa ttgcgtgctt gtgaaagttg ttataaatca
    14461 aataagtggt tgtgaaattt gcactctgaa aaggacgtct tatctttaaa taagtggtag
    14521 cgaatcgcta cggaatagag ataacacgag gagtggttag aaatggctaa agttctggtg
    14581 ctttattatt ccatgtacgg acatattgaa accatgaagc attaaaaaca cagaattacc
    14641 gaagatcgtc caagagcata tatttttcaa tctgttacac ctcattttga ccatgaagaa
    14701 atcaatttta caccgttctt tatacgttct tttttgcccc aaatatgccc caaacaacca
    14761 cattttgccc caatagtgcc ccaaacaacc acattttgcc ccaatagtgc cccaaacatt
    14821 taaggggatt taacttgata tttcagataa ttaacgcaag tttgcgatgc gaatttgcgt
    14881 taattttctg gatgaggcat ttaccgttac ccgttcatca atattccgct gattgacaaa
    14941 tcctccagag ctatcgcata ctgaccgcac tgaacaaata agcggtctcc gcacccgata
    15001 gctttgcggc ttttttatgc ctgcaatttg gcatagttac atccgtacaa aggtcgggtg
    15061 gagaggcgta atacaacacc cgcaagggga atatgcccag agcttcttat ttggctcagt
    15121 tgacacccga ccgccagcta ctaactggcg gttataaact aaacaaataa ggaggtcatc
    15181 atgaccagtc aacgcatccc cgtattcaac ggcactatcg ccaacgaaac cactcttctc
    15241 gttaatgccc gtgatttaca tacttttctt ggagtaggta aacgctttgc atcgtggatt
    15301 acagaacgta ttgaagagta cggattcgtt gaaaatcagg actatatagc tatttcccag
    15361 aaacgggaaa taggttatgg gcgagggaaa aaagactacc accttaccct cgatacagcc
    15421 aaagaaacgg cgatggtaga gcgtaacgaa aaaggccgac agatacgcag atacttcatc
    15481 gagtgcgaaa agaaacttcg caacatgcag tcggcacaag cagaaccaca gcaacaattc
    15541 acagacgagg aaatcatcct cctctgctac atgcaactct ggatggaaaa agcccaggac
    15601 ctcagcaaac acctgtatcc gatcatgaaa gagctgaact cctcatacac gaacaagctg
    15661 tatgacattg cgtttgagac catctacatg gtgacgaaga acagagatgt gctactgaga
    15721 gaggcggcac ggcttgacca gtcgagtttc gtagttcagc gggccagacc aatgctgaaa
    15781 agcctgcggg caaggcaatt cgaattctaa aaccaaagga gcttcggctc cttttttcat
    15841 gcctgaagga aaggaaaatg gcagatatca tcgacaacgc cgctgaaatc gaagaattgc
    15901 agcgcaatct ctccctgcaa aaatacaaat ccgatagtaa tgccccatct gctactcatt
    15961 gttg
//
LOCUS       DAZFKY010000105        15780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFKY010000105 DAZFKY010000000
VERSION     DAZFKY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHKCL_002994"
     CDS             complement(<1..281)
                     /locus_tag="ACHKCL_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5763918.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAE"
     gene            complement(291..620)
                     /locus_tag="ACHKCL_002995"
     CDS             complement(291..620)
                     /locus_tag="ACHKCL_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5763919.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(620..3685)
                     /locus_tag="ACHKCL_002996"
     CDS             complement(620..3685)
                     /locus_tag="ACHKCL_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5763920.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(3657..3986)
                     /locus_tag="ACHKCL_002997"
     CDS             complement(3657..3986)
                     /locus_tag="ACHKCL_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5763921.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(3995..4381)
                     /locus_tag="ACHKCL_002998"
     CDS             complement(3995..4381)
                     /locus_tag="ACHKCL_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5763922.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(4442..5185)
                     /locus_tag="ACHKCL_002999"
     CDS             complement(4442..5185)
                     /locus_tag="ACHKCL_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5763923.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(5196..5597)
                     /locus_tag="ACHKCL_003000"
     CDS             complement(5196..5597)
                     /locus_tag="ACHKCL_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5763924.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(5594..6172)
                     /locus_tag="ACHKCL_003001"
     CDS             complement(5594..6172)
                     /locus_tag="ACHKCL_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5763925.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(6184..6459)
                     /locus_tag="ACHKCL_003002"
     CDS             complement(6184..6459)
                     /locus_tag="ACHKCL_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5763926.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(6452..6775)
                     /locus_tag="ACHKCL_003003"
     CDS             complement(6452..6775)
                     /locus_tag="ACHKCL_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO5763927.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(6862..8889)
                     /locus_tag="ACHKCL_003004"
     CDS             complement(6862..8889)
                     /locus_tag="ACHKCL_003004"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5763928.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(8834..9169)
                     /locus_tag="ACHKCL_003005"
     CDS             complement(8834..9169)
                     /locus_tag="ACHKCL_003005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5763929.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(9291..10415)
                     /locus_tag="ACHKCL_003006"
     CDS             complement(9291..10415)
                     /locus_tag="ACHKCL_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5763930.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(10343..10555)
                     /locus_tag="ACHKCL_003007"
     CDS             complement(10343..10555)
                     /locus_tag="ACHKCL_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763931.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(10552..12654)
                     /locus_tag="ACHKCL_003008"
     CDS             complement(10552..12654)
                     /locus_tag="ACHKCL_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5763932.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(12654..13145)
                     /locus_tag="ACHKCL_003009"
     CDS             complement(12654..13145)
                     /locus_tag="ACHKCL_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5763933.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(13820..13972)
                     /locus_tag="ACHKCL_003010"
     CDS             complement(13820..13972)
                     /locus_tag="ACHKCL_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763934.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(13960..14427)
                     /locus_tag="ACHKCL_003011"
     CDS             complement(13960..14427)
                     /locus_tag="ACHKCL_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5763935.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(14424..14921)
                     /gene="rrrD"
                     /locus_tag="ACHKCL_003012"
     CDS             complement(14424..14921)
                     /gene="rrrD"
                     /locus_tag="ACHKCL_003012"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5763936.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(14921..15136)
                     /locus_tag="ACHKCL_003013"
     CDS             complement(14921..15136)
                     /locus_tag="ACHKCL_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5763937.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            15279..15677
                     /locus_tag="ACHKCL_003014"
     CDS             15279..15677
                     /locus_tag="ACHKCL_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763938.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            15692..15780
                     /locus_tag="ACHKCL_003015"
                     /pseudo
     CDS             15692..15780
                     /locus_tag="ACHKCL_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3589 a         4684 c         3630 g         3877 t
ORIGIN      
        1 tcttccgcca tccccgtgac catgccgtgc aggttagaaa ccgtcagtgt cggacgggca
       61 gaactgccct tgccgttcag ttcaaatccc gtcccctgaa tggggtatgc ctgatactgc
      121 cgcccctgcc aggtaaccgg ctcacctttt tcgttctgct cattacagaa aaaataacgt
      181 tcaccaccga cctctgtcag atcgatttcc cagagcaccg cctgggctga ctgagtgagg
      241 cgtgtcgtct catgatgtgt ttcctgtgga atatcctgca tcagagcctc ctatgccacg
      301 acctgttcaa aatctgccgt tatggttacc cacagcgccc ccacgcttgc cgaccattta
      361 cgacaaacca ccctgatcgg tttccagtca taaggtggcg tccactgaaa tgcacggacg
      421 ccaccgtgcc gttccagaaa ggcttttaaa gatgggtgtt cacatttacg aacacgtatc
      481 gtcacgctgt aagtcgacaa ctggttattc agtcccgccg cacgacgctg ttcataacca
      541 tcgcccagct tcactgtcac cactttcggc tctgatacca cattcatatc cgggcgcact
      601 ttccagtgaa acgtctccat taccgatatg ctccacttaa ccgaccacca tcacgggcct
      661 gctgttgcat aaagtccgct gccgcttttt tcccgaggtc ataaaccgcc ttcagggcag
      721 ccggacctat ctgcccgttc gtgccatcgt tattgatctc gatgttgtac tgcggggaaa
      781 acatcgccat acctgaaccc ccaatatccg ccacaacccc cagcttaccg tcagcaccac
      841 gacgcaatgg cagaatggct tcaggtcccg cttcccccat cacacccgcg ccttttgcaa
      901 aagcaaaaaa cgtcggacgg ttaaccaccg tgccactgta acgactcaaa tcagcagact
      961 gataaatccc cccattggca ttcagggaaa ggctgctgag atcaaatccc agtacactgc
     1021 caatcccttt tatcgatttc atcatggttg cctgcgccag gattttcgcc atatctgaca
     1081 gcacagaaga ggtgaaggat ttgaaattga gtttgccggt agtgacaaaa gttgccaggc
     1141 catttcccat gttgctgaag gctgacgaga acacctgttc tgctgtttct gccgtattat
     1201 ctgcatccac ggtaaaatcc tgaaatgcac gcaggactcc gtttttccag ttaccctgta
     1261 caacttcaag ctgttgccag taacggcgat tctcattcag ttgtcggttc aggctctccg
     1321 tcagcgcctg ctcggccttt ctgtagtcat ccgtgttata tgtccctttc tgctcactat
     1381 cccgcctcaa ctgctccagc tgttgctggt atttctggcg aagactcagt tgtacctgat
     1441 atcgctgtcg ctgctgatca cccataccca ccgtggcgat atccaggtca tgttgctgac
     1501 gctgagcgcg ctcttcttca gccagttgac tggtcagctg aattgttttt ttcttcagct
     1561 cgttgagtgc cgtctgtttc tgaagctcct gctgttttac atccagcagc gtcagtgcct
     1621 gaatcagttc atctttacgg gccagcacac tcttttcatc tgccgtcagt tttttcccgt
     1681 ccaggtcgct gatgcgctgc tgcagagcca gaagctgttt acgcgcttct gtcatccttt
     1741 ccgtggcaat gcctgctgac tgtctggcag cagcaatctg tccttccacc tgtgcctgtt
     1801 gctgactgta ctgcagcaat aaccgggtgg cctcatcatt acgggtttcg cgtgtttttt
     1861 tcttaccgga tgccagggct ttctcgtaac gttcattttc acgttgtatc gccgcatccc
     1921 tgacagcctg atcggcgtac tgcatggcat taatacgcgc tatttcacgc tgatgtcgtg
     1981 ctgcttccgt ttcattcatc cggttcagtg cagcattttc agcattacgg cgtttctgtt
     2041 gctcctgata attccgctct gcctgctctt ttgcatcctg caaatccttc tggcgttttt
     2101 tctcctgaag atcgttaaga cgctgctgat cgtattcaac ctgagaagat gatgccgtcc
     2161 aggggagtct tttcgcccgc gacactttct cctgtaaagc ggcaatctgt tcatccagcg
     2221 agtcttcacg accaatattc atggccgcat cccagaaacg actccacaaa tcagacagat
     2281 acttcagcgt actgccaagc gcattgaggt tattatcaat atccgcagta cgccgaccgg
     2341 tttcctctgc cagtgcagac atggctatcc gtgcagcatc actggaccgc ccctgctccc
     2401 caaggacgcg tatctgctca agctgagtgg cagtaagaaa atgcagctca ttgtccagag
     2461 ccttcgcggc atttacagga tcatccttca gccgcttaaa ctgatttatg gtatcgctga
     2521 ccgactggcc aaccgatcgc tccatctgtg cggcagctct cgccaccata ccgatatcgt
     2581 ttccacgaaa tgcaccactc cccaccacct gagccagcgc accggctgca gcatgttgcg
     2641 tgataccatt cccggaaata gcacgactga gcgtccacag ctgcccggca gtgactccgg
     2701 cataatgccc cgtcagcgac agctggcggt taaattcttc cccctccttc tgaccgtcat
     2761 accaggcttt acccagacca tagacagccg cggcaatacc gccaataacc gctcctggca
     2821 tcatgccttt cggtgacatc agtgtgtcta tccacccggc acggttagcc agcgttattc
     2881 cggatcccct cagcgcacct aaattgccgc gggccagttc acctatcaga acgcctatct
     2941 cctgacgggc tgctgcactt ttcagaccca gcgaatgcgt ggcttttcct gcctgctcca
     3001 ttttgcggat atacacttct gcagcactgc ttacccccag ctgggcagcc ttagcacgaa
     3061 gcaactcaga agaagaaaga ttctggcggg ttgcctgctc tttaagctga cggataaacg
     3121 ccactttctg tcgggtagcc tcttcctcag cctgtgtaag aacacgggtt ttcgccgtaa
     3181 cctcagaaat cagcgccaga taatcctgct gaccaatccc gccactgttt ctggcctgtc
     3241 ggatctgttg ctgaatacgc tgtaattcct gcagccccgc accggcctgt ttcacactgt
     3301 caatctgacg ataaaacgca gcagccgctt tatcctgagc ctccgccaga gccatggcct
     3361 gcgcctgttc ctcgcgcatt ttctggctca gtgcctccat acgctggcgg gtttgctcca
     3421 cctcgcgggc catgcgttca tgagcctgtg cgctcttctc caccgtctgc gcatggacgg
     3481 atgcggctgt tgcagccgaa gaagccgcct gcattgtctg ccgggcggcc tgagtctgac
     3541 gctccataaa acgctgcata cgggcagaag accgttctgc atcgctggct gcaccattca
     3601 gaaggttttt gatacgggga atttcatttt taaactctgc cgcatcaatc cccaaatcaa
     3661 tgaccaggtt ggctatctgg tccataacgc acacctccgg aaataccttc cccaagatgc
     3721 atcagttctt cgtccgttcg ctccggtatc ccgttctctt ccggtaaaag gctgaaatca
     3781 gccaccgcag catcactgct accggacacc attctcacga tcaatgcctt cagcgaggca
     3841 aactgcgcat ccatccacac atcactgaag ctctgcatcc ggaaataatc gccccactca
     3901 ccaagctcag tggccgacat ttccgacagc atccgccgcc agtctgcccg ccggaactcc
     3961 cgggcaagcc gcatgacaaa ctgcatttcc cgcgtcagga cttttccggc gtcagcacct
     4021 catgctccaa atccccggca ttctcaatgg ctcccatacc gctcagcgac agaaccatct
     4081 ccgcccccgc tcccagggca tcatacgacc atgttgtaat aacggatgcg caaagcgtct
     4141 caacatcctg agactgttcc gcattccaca gtgagcggga aaccagccaa gcattgatat
     4201 ccatccccat ccgcagaaaa gcaatctgtc gttcagcctc cggcagttct ccctcttcgg
     4261 catcaaactt tgccgttcgc tgctgaacaa acgccagata ttcaattctc tgcagcccgg
     4321 acagctcact gagcaccacg gactgctttt cataattaaa cgtgccctgt ttcagaaaca
     4381 tcatgttctc cacctgcaaa aaagccccgg ataaccgggg caaatgatga gtatcgtcct
     4441 gttaacctgc agcgctgaca gtcaccgcag ccacagccac aaaattcccg tcagcggtca
     4501 tgcccacaat gctgacgctg ccctgcttca cgcctttcac cgtggccaca agtccgttca
     4561 gggtcacagt ggctgtctgt ggatctgtcg aatgcacact gatcgctttg tcactggctc
     4621 cgtcaggttt tactgtaaag gtcagcgtgg tggttgctcc cacattgaca ctggcggatg
     4681 ccggtgccac cgtcagcccg gtaacgccca cagtttcagt gccctcctct gccagatatg
     4741 gacgccccac accgctgatt ttcactgtgc gggtcatcac gtcttttgag gcaatggttt
     4801 tacccagtga gctcagccag ccgcggaaaa catcaacagt gccgttggga tatttgatac
     4861 gaaacgcgca gacttcaccg gagtcgaaca actgaaccag ttttttctgc ccgctgtcac
     4921 ccggacgcca ggccagcgtc gccgaagtat caccaacgga tttctgcccc tgggttgtcg
     4981 ttttccagtc tgcatcttca tcatcgagat aagtgtcatc ttctgcatca gcggtcattt
     5041 cgccaggttg cagatccttc accatcgcaa gacgcagcca gtcagtgtcc gacaaagggt
     5101 tcgcaaacgc atcgccgttg ccggtataca tccagaacgt cgtccccgca cctttcgtct
     5161 ttgccagtgg atttggcgtg gtcattacca cctccttaat tcgtgtacgt gatctggtac
     5221 gtgatttccg ccatcgccca ggtggccatc tcattatcac gttgatagtt aaaaccgagt
     5281 gggatcaggg tgtcgatgag tccggaaagt gccgatacat cattcagggc cgggaaaatg
     5341 gtgctctcca tccacatatc cagctctgaa tccggtgcct gtgcccggat gaagacagca
     5401 atatgcagaa cagcctgcca gtcatcttca tccgtcattt ttccggtgta ctgagcatca
     5461 ctcagccaca ccgccacggc aggcagttcc tgcgcatcaa caaatgccgg aagcccgtca
     5521 aaaaacgtgg cgctgtctcc acactgttcc cgaaggcgtg ccagtacgac ctggcggatt
     5581 tgtgtatgtc ggttcatcgg gtcagccata acctcagttg ttgtttcagt gcatacccca
     5641 gctgtttcgg catttccgca gcaatgatgc ggtcgcgggc atcttcaaat gcctgtgtca
     5701 gcggtccgga cagcgggatt ttcaccacat caatggggta acgatttttg ccatcaatac
     5761 gccgcatcac atgccagcga ccattcgcca gttgctgaat aaacgcatcc cggaaaagat
     5821 atttacccac cttcagcaca ctgccacggt actgcagttt tccaccacgc tgggccagtc
     5881 tgacccgggc tgtccccagc ttaatggcgg gcagattgcc ccggttaacg cggatcctgg
     5941 ccgtcatttt tcctgacgga ctggctttaa acacccggac acgctgacga accagtttca
     6001 gggggatccc tttcacctgg ttatctcccg caacggtatt cccggcaacc tgccgggtgg
     6061 caaccgagcc cgctttctgt gccacacggt ttatcgccca tgcgctggcc tgtggcacca
     6121 tacgggtatc aaggctgttc agattgcgga tggcattctc aagccccttc atcccacacc
     6181 tctttactca ataaagatca ttggcttacc gttaaagcgt tcatgccgtg tgaccgtcca
     6241 ttgttgtccg tcataaacaa cgcgatcccc gcgccgtggg cggtatcccg aagaaaacac
     6301 caccagagag accgcaggtc cggacagagc attcagctct gccagtgttt ctcccgggat
     6361 cacagtcata tcgacatcat taatcgaggc tgtctttccc atctttctga ccgtgatagc
     6421 atccatacgc gctgccagcc gggaaaaggg atcagacatt gagttttacc ggcacttctt
     6481 ctgcactggt tccggcatct gcccagacaa ccccgaccag cggatcagag ccgctgttag
     6541 tcagctgaac ttttcctgac ttcagataaa ccttcttacc cgttttcatg tcatccgttt
     6601 tcagcttagg cagcataaac acaccttcgg tcatgccgtc gcctgtttca ccctgtggaa
     6661 tatcggtcag cgccaccgca aaaacatcgc ccacctgcac cagatctccg ctgctgatgg
     6721 ctgcactggc aacaatcgcc accgtttttc cttcttctac aaaattcttt gccataactg
     6781 tctccgcaca gccccgttca ggggctgatt tcaggtacaa aaaaagccct tacgggccat
     6841 cagagttgtt gtctgcgacg tttacgccgt acatttcacc agaccgcggt gatcaactgg
     6901 cgcgacaccg gcgtcaatac gcactttcgt tgtcacgcca tccacactga agccctccat
     6961 ctgatcaata tatggcgtat ccacaccgtt gagataagcc acttcaatcg tatcggagcc
     7021 ttttgacgca gccaggtaga aggtggtctg gctgttatca tcaagacgag gctctgcaat
     7081 aacggtcgca aaatctttca ccgggttaat aataccggcg ttaatgtcag cccccttgac
     7141 acttgaggag cgaatgacct ggttagcaac agactccatc gccgtcggta ccagtacgaa
     7201 cgcaggacga atattcagat gacgctcccc ctctttctga acgcgcatca actggcgggc
     7261 tttatccagc gatgccacgt ccattgcagc gctctccagt acgtttgcat gtttcgcttt
     7321 atcgaacaga cttacattat ctgtggagat tttcgggtta gacgtcagaa tggcataaac
     7381 cagatcggca atagtggatt tcgccgcacg gcccagtttc atcgggacat cggtcagcat
     7441 attcagatca tcattgataa tggcctgacg ggtgatactg aacagctcgc cataggtcgc
     7501 cagtgcaata gtggcctgtt tatctccggt ggtgacgtat ttatattccg ccccttcacg
     7561 cacctgacgc agagcactga agccccccat acccacacga tgggcaattt taaaatcaga
     7621 caactgacct ttccgcgtcc actgttcata ggtttcaggg gcatcttccc agccctgcag
     7681 aatggctttg ttcgcaacat ccagcagaat attaccgaag tcagacgtac tgtgtgtgaa
     7741 cgccgcaccg accatctgca tcgggttata actggaaacc ccaatacccc gttcagtcag
     7801 tgacatacgg gcatattcac gcagggtcat cccgttgtag acattatcac gttcggtttt
     7861 ttcaaatccg gcacgcgcca tcagcgcctg gcggatccca tcccccacaa aattaccgtt
     7921 tccggcataa atatgagccg gggtattttt attagatggc gtggactcgc gccccatctc
     7981 gttcaacagc ttttcgcggg cctgctccag cgaacattca ggatcggcaa gacactgagc
     8041 ctgcagcgtc tgataacgcc cgccaaacat ggcaaacaga tcattaatac cgtttacacg
     8101 cgctttttgc tctgccagta cctgcgcacg gatactgttt tcatccacca caggtgctgc
     8161 tgcctgcact ggcgtccggg aggctgcagg tccatcatcc tgtacgcgtg gagcactgtt
     8221 gcgtggcgga gtaatcatgt ttcgaatgga ttccggcatc tttttaaatt cctctgtacg
     8281 ttttgactga atacatgcca ttgccttaac ggctggcgtc acctgatcag caaatccatg
     8341 tgccagacat tcggcaccgg acatccaggt ctcatccgcc agcatggcag caatttcatc
     8401 ggtggttttc ccggttttct gtgcataagc gggtaacaga accgcctcaa ctttatcgag
     8461 caggtcggca taggtgcgca tgtcctccgc atcaccgccc gtaaagccaa atggtttatg
     8521 aatcatcatg aaggagtttt ccggcataat gaccggtttt cccaccatcg caatgaccga
     8581 cgccattgac gccgccacac cgtcgacata aacggtaatg gacgcaccat gtgttttcag
     8641 tgcattaaaa atggcgatgc cttcaaagac atcgccaccc ggtgaattga tattgagatt
     8701 aatgtgggtg atatcgccca gtgcattcag atcactgata aactgcttcg ctgtaacacc
     8761 ccagaaacca atctcgtcat aaatataaat atccgcgtca ctctggtgac cagcctgcat
     8821 cctgaaccag gaattattct tcggactggt cgtcggtgtg ctgcggctcc tgtcgtttcg
     8881 ttgcggcact gctgcctcct ttatcactgg ccggatcggt atcaaatacc agatccagct
     8941 tgcggttttc atcaatttcg gccttgcgcc gacgtttgac atcatccgga ttacgaccac
     9001 ctgcacgtac ccagtctgat tctgtcgctg ctccaccacg aatctgaatt ttccaggcct
     9061 cagcctcctt aacagggtca atccacggca tcaccggtcc ggaatacacc gcggtataca
     9121 gtgaagaacg gtcaagatcg cggggtagcc tgataacacc ggatgccaca gcctgtttca
     9181 gccaggcacg atacatcggg cgggtgacgg caccaataaa ccagtcctgc aggatcaggt
     9241 agccatcagt ggattcaacc agctcctgac gctgggcgct gtaagtgccg ttatagttgc
     9301 gcgctgtact ggaaaaaatt cagacgactg cccgccgcca cggcacgcaa ctgaccatta
     9361 cgaaaagttt caaggttagg attggggcga tccgacttca ccattccgat ttcttcgccg
     9421 ggtttcagat cgtcgtaaat aatgcctggc tgaatggtaa gctcgcgttc cttatccttg
     9481 ctgccattac catccggttc atagctctgc ccatcgcctt tccggatgta catccccaga
     9541 gcagcggcga tccttgctgc agtcagctca gaatcctcat actctttcag ggcactgagg
     9601 cggatcagca caccggacaa caaagacgtc ccgcgcatct ggtgcagacg gcgaacaaat
     9661 ttaagatgca gcattcgctc tgcatccact tctttggttt ccatctgccg tccggatacg
     9721 ggacggcttt tatacaccag atatttttcg ggacgccccc agtcatcaac aaacacgccc
     9781 tgattcagcc tgttgctctc atcactggtc atgggaataa agtctggctc gagcgcctcc
     9841 agccagaaat gaacaccggc agaaggcgtc aggctgttta tgcgcccgga aaccatctgg
     9901 gcaaacacct caccatcgcg cagccaggta cgcagcatca gacgttccag catcggacgg
     9961 gtaaactgcc cggtgacttc cggactgaca gaccattcac tccatcgggt gcgtatctcc
    10021 gctgccagat cacgggcaat ggccccattg cgtaataccg gatgtggctc gacaataatc
    10081 ccgtttttcc ccaccacccg ttcttccagc ttgtcaaata caccaataac cagatcgtgg
    10141 ttgttatcaa ggtaacgggc ctgctcacgt aacgacacgg ccccgtactg gcttaactgg
    10201 tcggcagttc ggttttcccg ccgggctttg tgtgtccgcg tcgtttttac ggcctcataa
    10261 gcctggatca ccgcacggga acgcagcctt gccgctttcc atcctggtga aaaaacgcca
    10321 atcacatcat caagaattgc catcagaacc tcgccagccg gtacccggga tgcccccgtc
    10381 gtcgtgtaat cagagccgca aggcggcgct cccactcctg ccgtccctgc cggatctcag
    10441 ataagttttc catggtcatc tgctgaccat taaaggtgac ggattttccg tccagcaccg
    10501 ccatttcagc ttccgtataa cgctgaatca tggcttcgat atcattctgg ttcataacca
    10561 tcctccggaa gtcagccagg ggttaacatc gtcagttact gttttcttcc gtttttgttt
    10621 tttaacaggc gtggataccg gttccggtga gggtgacggt tcggtactgt ccgggacact
    10681 ctccagccag gtttcccggc tcgcccaccc cggtgcatcc ggccagcgga tcttttcgta
    10741 tccatgcaga atgaccagag cctcggcata caccatcagg tcaaaagctt cgttggcacc
    10801 gcgacccggc ttactccatt tcccgtcact actccgctct tcatacgtca gttcgtcgta
    10861 aaaccagctc cccagccagt cagggaaatg cacatagccg ggacctggag agtcacgcca
    10921 taacgcgtta ttcacccggt ctttcagtgc atccgtctga agaagccaga gcggcacatc
    10981 acctgcggcc tgcgcccgtc ggcccgttcg tccggtgtta tcagggaatg tacgggtgat
    11041 cagttttgcg cgccggatgc tgtcgccctt aaacaggtaa atacgtttac caaggccatc
    11101 acgacggcaa cgacgccaga atttataggc attatcagtg accccgtctt caccgccgga
    11161 gtccaccgcc attgccatca gtcgcatttg ttgagaagga tcggaggcca gcggccagct
    11221 tttatgaaaa acatccgtca gcaggacatc ccagtcttcc ggatagctgg ccggatcaat
    11281 tcgctggctc tcaccgtcgc tgtcaccgcg caatgactgc gtgatgttgt aacgatcaat
    11341 aatccagcgt tcgccacggc tgccatagcc cgttacctga accacaaaac ggcgatgacg
    11401 tcccgcctgc acatccactg tcgccacaag gaaattaacg ccatccggca cactgctgga
    11461 aggaactggc tctgcccgct gctcaagcag ttcacttttt cgttgctcca tgctggcgcg
    11521 gggaagataa ggtaatcccc agtcggtatt gataaccgtc ttgagtgttt cttcacttcc
    11581 ggttgtctcg tattcctgtt ctgcagtaag cagtttgtaa acgagttgcg agagtgtctg
    11641 gtaagcagct gccggaccct ccatccagaa tgacgcaata cgtgagcgtc ggggatcacc
    11701 ataacgacta ccatccgcat tgatggattc accatcccgc aaccagaccc cacgtccgtt
    11761 cagctcacgt ttttgttcag gcataatccg tcctgaacag gaaggacact gaatataagc
    11821 cgcctcactt gccagcacgg gatcggcaat atcacggaaa ccagcaacca catcgccgca
    11881 gggctgaaaa tactcaccac agtgtggaca gggccagtac cagcgacggc gatcgccacg
    11941 gttatagagc gacagtatcc ccgtggttgg tggagcctca tgcggtgaag tccgtcgcca
    12001 tttcacatcc ttcacatccc tgccggggga actctccacc agcgtcatac cactggacat
    12061 aaatgtggtg gtacgttttg aggcaagaga gaaggcatcc ccctcgccat caatatcttc
    12121 cggaaaacgg tcataatccg tcagcgccac gcatttataa tctgatgagg acatgatatt
    12181 gactgacggc cagccgattt tcaggtagtt gccagcaagg aatgttctgt cataaacgtt
    12241 gttgtcattt ttgttcggac tcaggcgact gaccacttcc gggctgacgc gaaacgttct
    12301 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa tgatcagcat
    12361 atccgcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
    12421 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
    12481 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tcccggcccc
    12541 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
    12601 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
    12661 tcctgctgta attcctcctc atcatccgca cctgcttcgg tcacccgcag ggctatctga
    12721 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccacag
    12781 tcacgttcca gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
    12841 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
    12901 agacgctcac gttcagactg ataccaggct ttgcgttcat gtggatccat ttcgccctca
    12961 gcaaccggcg gtggtaaccc cataaattca gtcagaatat cggtcaaccg gtatagcttg
    13021 agtttgtcat gtccaccagc gggacgaatg tttttcagtc ttgccacgac agtctggcgg
    13081 tgcagaccag ataaagccgc cagttgatta atattcagca ccaggttttt caactcatga
    13141 tccatatttc ctccggagag ctttaaacat gcatcgtgcg aacaacttta agaaaacgcg
    13201 ttcgatgtcg aacaaaaaac actcaattcg acatacaaaa aacaaataac cattaataat
    13261 caataagatg caaagatgat ggtggccgat aaaaatgcaa aaactagcct ttttccgcga
    13321 cgctcccgcc ccgtggcagg ccaccccacc gggaggagcc gtcagcctga aagccatgac
    13381 gaacgtctga tacagccctt gcatgaatgg catcgggata atccagaaag gaatagcatc
    13441 gacccacaag aatctgtgtg agtgtcctgt ttcttccacc cccgcacagg actggcgagc
    13501 atgagggaca aacccgcgaa tcattagcgc ggtaaaaacc cggtgtgcat cgtttttgat
    13561 tattcccgca cactcgcgca gaaggagttc cccgtcgggc tacggtcatg gttaatgcgg
    13621 gaatacagcg acgatacagc gcatgatgtg tcaggcttga atacctttat ccgttaaaag
    13681 ggatatcagt taagttatcc cgtgtagggt ataagccatt atcaaagcca ctctgtaggg
    13741 agtggctttt gtaatggcaa taaaaaagcc ccgcgaatgc gaggctaaat ccaggtattt
    13801 ttaatgactg gctcttatct caacgcagcc ccttaccgcg cgccagatgc tcaatatcaa
    13861 gcatcagcaa tgagatgttt aaccatgatt cactccagaa gtgagcacca ccctgtctac
    13921 agagccagat gtgaaggatg atgagtaaaa ttatcgctat catcgaaggc attgcgtcct
    13981 gatgtattcc tgaagcgttc tcagtgctgt ttggtcgcgg ataattccgt cccggatacc
    14041 gagaacgttt cgtccagcaa ctggagagag ttcgacggtg gcatcattgc ccatgccgga
    14101 ggcgctggag gtttcgtctg aggatggcac agggcatttt cctttgacga acacccgacc
    14161 accattatca agcttgcgcc gaagagcatc atttttagct ttcgcatcag ctaactcctt
    14221 cgtgtattta gcatcgagtg catcagcagc acgctggcgc tgctgcatgt cagtaatggt
    14281 ggcggtcgcc tgcttcagct cactgacttt tttatcacgc tgttctttat aggcgatggc
    14341 gttatcacgg taatgattaa ccgcccatga ctggcagacg atgatgcaga taaccagagc
    14401 ggagataatc gcggttactc tgctcattgt tgcccccaca aacagacttc acgctcaatc
    14461 tcgcggcgag tcatcagccc tttccattgc ttaccgccag cgtatgtcca gcgccgtagc
    14521 tgatcacatg cgcctttgat atcaccctgg tttattttgc gaagaagcgt cgatgttctg
    14581 aaattaccag cgcccacgtt gtaaacgaac gagtaaagag cgccgcgcgt tgtttccggt
    14641 atatcgactt tgatgtacgg gttaatttgt ctggcgaccg tggcaaggtc tttattcagg
    14701 agggctttgc attctgcttc ggtatacgtt ttaccgggaa tgatgtcttt tccggtgtgt
    14761 ccgtgacata cagtccatac gccaacgata tcttcgtatg gtatgtagct gacaccttcc
    14821 aggccatcgt caccactcgg accagtgatg agcacagacg ctatggcagc agccccacca
    14881 ccaatagcag cagcaacagc cttgcgtaat gatggcgaca ttattcacct ctcgcagcct
    14941 tacgcttatc ttctttaatc ttgaaataaa ggtttgtcag atacgtcagc aagccaaaca
    15001 ccaagctacc caatacgcct attgccaccc actgggatgg ggagactttg tccagcaact
    15061 gcagtagcca gtatcccgtc cccaccgctg acgtggtgta tgacacacct gttgttattt
    15121 tttccatctg gtacataccc cgtctcccgt tatccggaag ctgacaacaa taaaaagcca
    15181 ccagttaatt cctgatggcc ctgatgcata aacgtcataa tacctgactg ttatgattga
    15241 caataatgat aatgtttata tagaaaggtt cccgatgtgt gttacatatc atttctccac
    15301 ggggaatatc cccacgccag cgcagactct tttacccgtt ctcttctgcg ctggctcttt
    15361 tttattatgc tgctgcattt acctctggca ccatgctttc tatctcaaca caatacgtgg
    15421 tacttcttgt aaccaatatc ataacgatta atcgacatag aatttctccc gtgtacagga
    15481 acagagttaa aaagccggaa ccggaatcaa atcacaggat gaccatctgc cagtggcagg
    15541 tcataaaaaa aaggccgcgc catgcgcagc cagaactcac aaggaaaatg atagaaggaa
    15601 ataacattag tgatgtacgc atggcgcctc ccgctaagtt ctgcaatgat caaacagaac
    15661 tcgctacgtg cccttaaaac tcgatcattt agcccctcca aggaggattc accatgcggt
    15721 tgatttttta ataaacagta aacaaaaaag tcaagaatta ttcattctgt tctttcatca
//
LOCUS       DAZFKY010000106        15739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFKY010000106 DAZFKY010000000
VERSION     DAZFKY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(23..1381)
                     /gene="plaP"
                     /locus_tag="ACHKCL_003016"
     CDS             complement(23..1381)
                     /gene="plaP"
                     /locus_tag="ACHKCL_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5763939.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1371..1433)
                     /gene="yoeI"
                     /locus_tag="ACHKCL_003017"
     CDS             complement(1371..1433)
                     /gene="yoeI"
                     /locus_tag="ACHKCL_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5763940.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1648..2577)
                     /gene="yeeY"
                     /locus_tag="ACHKCL_003018"
     CDS             complement(1648..2577)
                     /gene="yeeY"
                     /locus_tag="ACHKCL_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5763941.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2623..3447)
                     /gene="yeeZ"
                     /locus_tag="ACHKCL_003019"
     CDS             complement(2623..3447)
                     /gene="yeeZ"
                     /locus_tag="ACHKCL_003019"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5763942.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3873..3923
                     /gene="hisL"
                     /locus_tag="ACHKCL_003020"
     CDS             3873..3923
                     /gene="hisL"
                     /locus_tag="ACHKCL_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5763943.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    3899..4022
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4069..4968
                     /gene="hisG"
                     /locus_tag="ACHKCL_003021"
     CDS             4069..4968
                     /gene="hisG"
                     /locus_tag="ACHKCL_003021"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5763944.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            4974..6278
                     /gene="hisD"
                     /locus_tag="ACHKCL_003022"
     CDS             4974..6278
                     /gene="hisD"
                     /locus_tag="ACHKCL_003022"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5763945.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6275..7345
                     /gene="hisC"
                     /locus_tag="ACHKCL_003023"
     CDS             6275..7345
                     /gene="hisC"
                     /locus_tag="ACHKCL_003023"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5763946.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7345..8412
                     /gene="hisB"
                     /locus_tag="ACHKCL_003024"
     CDS             7345..8412
                     /gene="hisB"
                     /locus_tag="ACHKCL_003024"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5763947.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8412..9002
                     /gene="hisH"
                     /locus_tag="ACHKCL_003025"
     CDS             8412..9002
                     /gene="hisH"
                     /locus_tag="ACHKCL_003025"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5763948.1"
                     /translation="MNVVILGTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9002..9739
                     /gene="hisA"
                     /locus_tag="ACHKCL_003026"
     CDS             9002..9739
                     /gene="hisA"
                     /locus_tag="ACHKCL_003026"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5763949.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9721..10497
                     /gene="hisF"
                     /locus_tag="ACHKCL_003027"
     CDS             9721..10497
                     /gene="hisF"
                     /locus_tag="ACHKCL_003027"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5763950.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10491..11102
                     /gene="hisIE"
                     /locus_tag="ACHKCL_003028"
     CDS             10491..11102
                     /gene="hisIE"
                     /locus_tag="ACHKCL_003028"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5763951.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11199..12176)
                     /gene="wzzB"
                     /locus_tag="ACHKCL_003029"
     CDS             complement(11199..12176)
                     /gene="wzzB"
                     /locus_tag="ACHKCL_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5763952.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12322..13488)
                     /gene="ugd"
                     /locus_tag="ACHKCL_003030"
     CDS             complement(12322..13488)
                     /gene="ugd"
                     /locus_tag="ACHKCL_003030"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5763953.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(13737..15143)
                     /gene="gndA"
                     /locus_tag="ACHKCL_003031"
     CDS             complement(13737..15143)
                     /gene="gndA"
                     /locus_tag="ACHKCL_003031"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5763954.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15344..>15739)
                     /gene="perB"
                     /locus_tag="ACHKCL_003032"
     CDS             complement(15344..>15739)
                     /gene="perB"
                     /locus_tag="ACHKCL_003032"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFO5763955.1"
                     /translation="PITIKHPNSVVYDHTMIGSGAIISPFVTISTNTHIGRFFHANIY
                     SYVAHDCQIGDYVTFAPGAKCNGYVVIEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGM
                     GAVVTKSVPAGITVCGNPAREMKRSPTSI"
BASE COUNT         4035 a         4015 c         3929 g         3760 t
ORIGIN      
        1 ggtttattat tagaaatgat gattatgcta cgtcttcgta ctgcggaacc gggttacgga
       61 agctcttggt gacgcaagcc aggtaaatca gaccgatagc agcccagatc agacccagaa
      121 ccattgagct ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg
      181 gcaggaacag atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga
      241 actgcgagat gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttgatca
      301 gcgccgtagc cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga
      361 tgatgttcat cgccggagta cgccatttcg ggtgtacata accgaagaaa cttttcggga
      421 acacgccgtc acgacccatt acgtacatca gacgcgctac gcctgcgtgc gccgccatac
      481 cggacgccag tacggtaatg gtggagaaga tcagcgcacc gacctggaac gctttacccg
      541 caacgtacag catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg
      601 ggaagtacag ctgcaggaag taagttgcaa agatgaagat catgccgccg atcagcgcgg
      661 tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacagattgc
      721 tgatgccgtc aaagccggta aaggagaagc acaggattgt cgccccggta atcatcggga
      781 taacatgcgc atcgccagac cagaatggac gagtgctcgc cagcgtacca gcgccttcac
      841 cttcaaatac gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca
      901 cgacgattac agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca
      961 taaaggccac cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa
     1021 aatagatttt cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag
     1081 accaacccac cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag
     1141 agccagcaga aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg
     1201 caatcaatgc gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac
     1261 caaaagtatc aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa
     1321 ctaacgtaag cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca
     1381 tggtcattct cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg
     1441 gattcctcag cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg
     1501 cctcttggta tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt
     1561 ttttagtcat tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa
     1621 tgagaggctt gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc
     1681 ataaacaccg gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag
     1741 tcctgcggtc tgttcattcc agccaatcca gacgggttca aagagagttt gggcgaccac
     1801 tttcttctcg attaacgcgc cactatcgag aaaacgttgc gccagataac ggggtaaata
     1861 gccgcagccg aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat
     1921 ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc
     1981 caccacaatc gcccgatatc gtttaatgat acgacggttt aatggttctt cttcctgggc
     2041 taacggatga tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc
     2101 aaactcactg gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg
     2161 agtcagggca tcccatgaac caccgagtac gccattgata aacttcaggc gcgtcacgtt
     2221 atgatgttga taaaaagctt caatgagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc
     2281 cacgccaatc accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag
     2341 ttctcgcacg gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt
     2401 gaatttagcg cggtgaccgc tacgatcaag caattgaata ttaaggtcgc tttccagctt
     2461 gtgaacggtg taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa
     2521 actgccttct ttttctaagg catcgaggat catcaacaca tccagcagtg gtttcataca
     2581 cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac
     2641 cagcggatcg ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat
     2701 aatcttacct ttgccgctgt ccagactatt tctgaattgc ggaggttcca gccccagtaa
     2761 acgggccatc tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata
     2821 gatgtgtccg cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa
     2881 atggactaaa ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc
     2941 gggatgacgc cccggtccga ccaggcccgc aagacgcaga atatcgaccg aagtaccggg
     3001 taaattgtgc agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt
     3061 acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgaactggt
     3121 aaaaataatt cggggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg
     3181 taaatagaac tcatcgccgg ggccgctacg acgggccgga agcgtaatga ccagcgcatc
     3241 ggcatccatc agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag
     3301 cagatagcta tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact
     3361 cccggtgact tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc
     3421 taacccgaca attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag
     3481 gctacgctag cgtatcaaaa ctgacaattc attctatgaa tgaaatcgaa actgttagca
     3541 aatataccat tgcctgatgc gctccgctta ttagtcctac aaaacatcaa tatattgaat
     3601 ttaggcaaca tttgtagacc agataaggca ttcacgccgc atccggcagg aacaaagccc
     3661 actttagtta cagaacacaa tgttcttgag ggttttaatg acatattttc acgattactt
     3721 ttttaaccta gtcactctta gccccctcct ttccccgctc attcattaaa caaatccatc
     3781 gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta ggttaaaaga
     3841 catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt aaacagcacc
     3901 atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac attcagatct
     3961 tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt
     4021 attgcgcggt tgataacggt tcagacaggt ttaaagagga ataagaaaat gacagacaac
     4081 actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc tcgcgaattg
     4141 ctggcgcgct gtggcattaa aatcaatctt cacacccagc gcctgatcgc gatggcagaa
     4201 aacatgccga ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct ggtaatggac
     4261 ggcgtggtag atcttgggat tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc
     4321 cgcgcccagg gtgaagatcc acgctacttt accctgcgtc gtctggattt cggcggctgc
     4381 cgtctttcgc tggcaacgcc ggttgatgat gcctgggacg gcccgctctc cttaaacggt
     4441 aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca gaaaggcatc
     4501 tcttttaaat cctgcttact gaacggttct gttgaagtcg ccccgcgtgc cggactggcg
     4561 gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagcaaacgg cctgcgcgaa
     4621 gtcgaagtta tctatcgctc gaaagcctgc ctgatacaac gcgatggcga aatggaagaa
     4681 tccaaacagc aactgatcga caagctgctg acccgtattc agggtgtgat ccaggcgcgc
     4741 gaatcaaaat acatcatgat gcacgcaccg acccaacgtc tggatgaagt catcgccctg
     4801 ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg gtgatcaaca gcgcgtagcg
     4861 atgcacatgg tcagtagcga aaccctgttc tgggaaacga tggaaaaact gaaagcgctg
     4921 ggtgccagtt caatcctggt cctgccgatt gagaagatga tggagtgatc gccatgagct
     4981 ttaacacaat cattgactgg aatagctgta ctgcaaagca acaacgccag ctgttaatgc
     5041 gcccggcgat ctccgcttct gaaagcatta cccgcactgt taacgatatt ctcgatagcg
     5101 tgaaagcacg cggtgatgac gccctgcggg aatatagcgc gaagtttgat aaaaccacgg
     5161 ttaccgcact gaaggtgtct gctgaggaaa ttgccgccgc cagcgaacgc ctgagcgacg
     5221 agctaaaaca ggcgatggcg gtggcagtaa agaatattga aaccttccac actgcgcaaa
     5281 aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg ttgccagcaa gtcacgcgcc
     5341 cggtagcttc agttgggttg tatattcctg gcggctccgc cccgctcttc tcaacggtat
     5401 taatgctggc aactccggcg cgtattgcgg gctgtaaaaa agtggtgttg tgctcaccgc
     5461 cgccgattgc cgatgagatc ctttatgcgg cgcagctgtg cggtgtgcag gacgtgttta
     5521 acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct gtgccgaaag
     5581 tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga ggcaaaacgt caggtgagcc
     5641 agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg ttggtcattg
     5701 ccgacagcgg cgcaacgccg gatttcgtgg cttctgattt gctttctcag gctgaacacg
     5761 gcccggactc acaggtgatt ttactgacgc ctgacgctga tatggcgcat caagttgccg
     5821 aagccgtcga acgccagtta gcagaactgc cgcgtgccga aaccgcccgc caggcactga
     5881 acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtggagatc tccaaccagt
     5941 acggcccgga gcacctgatc attcagaccc gcaacgcccg cgaactggtc gatggcatca
     6001 ccagcgccgg ttcggtattt cttggtgact ggtcaccgga atcggcaggt gattacgcct
     6061 ccggaaccaa ccatgttcta ccgacttacg gctacaccgc cacctgttcc agcctcggac
     6121 tggcggattt ccagaagcgg atgaccgtgc aggaactgtc gaaagtaggt ttctccgcgc
     6181 tggcttcgac cattgaaaca ctggccgccg ccgagcgcct gaccgcccac aaaaatgccg
     6241 ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta ttaccgattt
     6301 agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc tgggcggtaa
     6361 tggcgacgtc tggctgaacg ccaacgaata tcccacagcc gtggagtttc agcttactca
     6421 gcaaacgctc aaccgctacc cggaatgcca gccgaaagcg gtgattgaaa attacgcgca
     6481 atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggcgcggacg aaggtattga
     6541 actactgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact gcccgccaac
     6601 gtacggcatg tacagcgtca gcgctgaaac cattggcgtc gagtgccgca cagtgccgac
     6661 gctggaaaac tggcaactgg acttacaggg catttccgac aagctggacg gcgtaaaagt
     6721 ggtctatgtt tgcagcccca acaacccaac cgggcaactg atcaatccgc aggattttcg
     6781 caccctgctg gagttaacgc gcggtaaggc gattgtggtt gccgatgaag cctatatcga
     6841 gttttgcccg caggcatcgc tggctggctg gctggcggaa tatccgcacc tggctatttt
     6901 gcgcacactg tcgaaagctt ttgctctggc gggccttcgt tgcggattta cgctggcaaa
     6961 cgaagaagtc atcaacctgc tgatgaaagt gattgccccc tacccgctct cgacgccggt
     7021 tgccgacatt gcggcccagg cgttaagccc gcagggaatt atcgccatgc gcgaacgggt
     7081 ggcgcaaatt attgcagaac gcgaatacct gattgccgca ctgaaagaga tcccctgcgt
     7141 ggagcaggtt tttgactctg aaaccaacta cattctggcg cgctttaaag cctccagtgc
     7201 agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata aacaaccctc
     7261 tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga
     7321 cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga tcgcgatgga
     7381 accctgatta gcgaaccgcc gagtgatttt caggtggatc gttttgacaa actcgccttt
     7441 gaaccgggcg tgatcccgca actgctgaag ctgcaaaaag cgggctacaa actggtgatg
     7501 atcactaatc aggatggtct gggaacacaa agtttcccgc aggcggattt cgatggcccg
     7561 cacaacctga tgatgcagat attcacctcg caaggcgtac agtttgatga agtgctgatt
     7621 tgtccgcacc tgcccgccga tgagtgcgac tgccgtaaac cgaaagtaaa actggtggag
     7681 cgttatctcg ctgagcaagc gatggatcgc gccaacagtt atgtgattgg cgatcgcgcg
     7741 accgatattc aactcgctga aaacatgggc attaatggtt tacgctacga ccgcgaaacc
     7801 ctgaactggc caatgattgg cgagcaactc accagacgtg accgttacgc tcacgtagtg
     7861 cgtaatacca aagagacgca gattgacgtt caggtgtggc tggatcgtga aggtggcagc
     7921 aagattaata ccggcgttgg cttctttgat cacatgctgg atcagatcgc cacccacggc
     7981 ggtttccgta tggaaatcaa cgtcaaaggc gacctctata tcgacgatca ccacaccgtc
     8041 gaagataccg gcctggcgct gggcgaagcg ttaaaaattg ccctcggcga taaacgcggt
     8101 atttgccgct ttggttttgt gctgccgatg gacgaatgcc ttgcccgctg cgcgctggat
     8161 atctctggtc gcccgcacct ggaatataaa gccgagttta cctaccaacg cgtgggcgat
     8221 ctcagcaccg agatgatcga gcacttcttc cgttcgctct cttacaccat gggcgtgacg
     8281 ctccacctga aaaccaaagg taaaaacgat catcaccgtg tagagagcct gttcaaagcc
     8341 tttggtcgga ccctgcgcca ggccatccgc gtggaaggcg ataccctgcc ctcgtcgaaa
     8401 ggagtgctgt aatgaacgtg gtgatccttg gtactggctg cgccaacctg aactcggtga
     8461 agtctgccat tgcgcgtcac ggttatgaac ccaaagtcag ccgtgacccg gacgtggtgt
     8521 tgctggccga taaactattt ttacccggcg ttggcactgc acaagcagcg atggatcagg
     8581 tgcgtgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggtg ctgggcatct
     8641 gcttagggat gcaactgctg gggcggcgca gtgaagagag caacggcgtc gatttgctgg
     8701 gcatcatcga cgaagacgtg ccgaaaatga ccgactttgg tctgccgctg ccgcatatgg
     8761 gctggaatcg cgtttacccg caggcaggca accgcctgtt tcaggggatt gaagacggcg
     8821 cgtactttta ctttgttcac agctacgcaa tgccagtcaa tccgtggacc atcgcccagt
     8881 gtaattacgg cgaaccattc accgcggcgg tacaaaaaga taacttctac ggcgtgcagt
     8941 tccatccgga gcgttctggt gccgctggcg ctaagttgct gaaaaacttc ctggagatgt
     9001 gatgattatt ccggcattag atttaatcga cggcactgtg gtgcgtctcc atcagggcga
     9061 ttacggcaaa caacgcgatt acggtaacga cccgctgccg cgcttacagg attacgccgc
     9121 acaaggtgcc gaagtgctgc atttggtgga tctgaccggg gcaaaagatc cggcaaaacg
     9181 tcaaatcccg ctgattaaaa ccctggtcgc tggcgttaac gttccggtgc aggttggcgg
     9241 cggcgtgcgt agcgaagaag acgtggcggc gttactggaa gctggggttg cgcgcgtggt
     9301 ggttggctcc actgcggtga aatcaccaga aatggtgaaa ggctggtttg aacgtttcgg
     9361 tgccgatgcc ttagtgctgg cgctggatgt ccgtattgac gagcaaggca acaagcaggt
     9421 ggcggtcagc ggctggcaag agaattcggg cgtgtcactg gaacaactgg tggaaaccta
     9481 tctgcccgtc ggcctgaaac atgtgctgtg taccgatatc tcgcgcgacg gcacgctggc
     9541 aggctctaac gtctctttat atgaagaagt gtgcgccaga tatccgcagg tggcatttca
     9601 gtcatccggc ggcattggcg acattaacga tgttgctgcc ctgcgtggca ctggcgtgcg
     9661 cggcgtaata gttggtcgtg cattactgga aggtaaattc accgtgaagg aggccatctc
     9721 atgctggcaa aacgcataat tccatgtctc gacgttcgtg atggtcaggt ggtaaaaggc
     9781 gtacagtttc gcaaccatga aatcattggc gatatcgtgc cgctggcaaa acgctacgcc
     9841 gaagaaggtg cagacgaact ggtgttctac gatatcaccg cttccagcga tggccgtgtg
     9901 gtagataaaa gttgggtatc tcgcgtggcg gaagtgatcg acattccgtt ctgcgtggcg
     9961 ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc ggataaaatt
    10021 tccatcaact cccctgcgct ggcggacccg acgttaatta ctcgcctggc ggatcgattt
    10081 ggcgtgcagt gtattgtggt gggtattgat acctggtacg acgctgaaac cggtaaatat
    10141 catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg ggaaacgctc
    10201 gactgggtac aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa catgatgaat
    10261 caggacggcg tgcgtaacgg ttacgacctc gaacaactga aaaaagtgcg tgaagtttgc
    10321 cacgtcccgc tgattgcttc cggtggcgcg ggcaccatgg aacacttcct cgaagccttc
    10381 cgcgatgccg acgttgacgg cgcgctggca gcttccgtat tccacaaaca aataatcaat
    10441 attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat atgttaacag
    10501 aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg actgatgccg gtgattgtgc
    10561 aacacgcggt atccggcgaa gtgttaatgc tgggctatat gaacccggaa gccttagaca
    10621 aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga ctgtggacca
    10681 aaggcgaaac gtcgggcaat ttcctcaacg tagtaaatat tgcgccggac tgcgacaacg
    10741 acacgctact ggtactggcg aatcctatcg gaccaacctg ccacaaaggc accagcagct
    10801 gcttcggcga caccgctcac cagtggctgt tcctgtatca actggaacaa ttgctcgccg
    10861 aacgcaaatc tgccgacccg gaaacctcct acaccgccaa actgtatgcc agcggcacca
    10921 aacgcattgc gcagaaagtg ggcgaagaag gcgtggaaac cgcactggcc gcgactgtac
    10981 atgaccgctt tgagctgacc aacgaggcgt ctgacttgat gtatcacctg ctggtattgt
    11041 tgcaggatca ggatctggat ttaacaacgg tgattgagaa cctgcgtaaa cgtcatcagt
    11101 gagttgcggg gtaagcggat gcgatattgt tgtcgcatcc ggcaaaaaaa acgggcaagg
    11161 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt gtaattacgc
    11221 agagcgttac gccccagcac aatccccgcg ccaaccatgc cgcccagcag caccgccaga
    11281 atcaaggtga ttgccttttt cggactatcg cgacgaatag gtaacgtcgg tttcattaca
    11341 tagcggtaag catgaatatc aagatcatca acctttaagt tttcaatatc caacaggttt
    11401 tgacgtgtct gatagtagtt tggtgagaac accaacggac gggtcgcctc atgcttaatc
    11461 atcgactcca gcgcttcgct ccccaaaagg aacaacgtat cttgcgtcac atcttcagtc
    11521 tgctgaacct gtggctttgc cacctgcgcc tgattcgcat actgcaacgc ttcctgaatc
    11581 tgacggatac gcagctcttt ctgctcctgc gcaactactt cctgcgttct taaagagtcc
    11641 tgcaagtttt tccgtcccag agcaatgttg tctttgagat ccttttctag ctcttgattc
    11701 actttatcat caacttgctg aatgtattgg gccaacttca tttgtgctcc ctctgcagtt
    11761 tgcccaacat aagaaacagt caatggtaat tgctggttct taacagaagg ttcgatggta
    11821 agtttttctg gttcttcctg attatccagc gtttctgcta atgctgagaa ggcagaactg
    11881 aagcgaccaa tcaacgtctc ctgcaaatcc gatactttcg gtgcagcctg accatagata
    11941 acattcatgg cattgttata gccagcaatt tgccccacgt cgggctgagt gataattgct
    12001 gttgacgtcc atttctcctt cgctactgcc aaataaccaa tagccagagc aatagccaca
    12061 atgacggaaa tgatgattgt catcttgcca cgccacaact gcactagtaa atcaatcaaa
    12121 tcaatctgtt ccgggtcatg gttttgccca gaaacattat tattttctac tctcatcatt
    12181 accctaactg acggaaattt ccaagtccgc atagtttagc gataatccct gtgaatgcta
    12241 taggataagt gataaaaata atgaattatt gcggaggtaa caaaagacaa tcagggcgta
    12301 aatagccctg ataacaagat gttagtcgct gccaaagaga tcgcgggtgt agaccttatc
    12361 tgccacatcc ctaagctctt ctgccatacg gttggaaata atgacgtcgg cttgttgttt
    12421 gaaggtggcg agatcacgtt ccaggcgaga gttgaagaat gagtcttctt tcatcactgg
    12481 ctcgtagatg attacttcaa cacctttcgc cttgatacgc ttcataatcc cctgaataga
    12541 agacgcacgg aagttatctg aaccgctctt cataatcaga cgataaatac ctaccacttg
    12601 cggcttgcgt gacaaaatgg catcggcaat aaaatcttta cgcgtgcggt tagcatcgac
    12661 aattgccgag atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc
    12721 tttcggcaaa caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg
    12781 tgggtcgaga caaacgcctt cgattatttg acgggaattc agacctaaac tttctgcgta
    12841 gctatccagt tcgttaaagt acgccacgcg catcgcgagg taggtgtttg caaaaagttt
    12901 aatcgcttct gcttcagtgg agtcggtaaa cagggttggg atattttgct taatcgcgcc
    12961 ttcctgtaat aacgcagcaa aacgttctgc gcgttccgaa cgctcaccga ttacaatacg
    13021 tgacggatga aggttatcgt aaagagcttt accttcgcgg agaaattccg gagagaaaat
    13081 aatattttca gtacgatatt tcttatgcat cgcttcggta aaaccaacgg gaaccgttga
    13141 tttgatgacc ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac
    13201 actggatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac
    13261 ataatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc
    13321 tgattgcaaa aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat
    13381 agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgag cgattagaag
    13441 cccgtttgac aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca
    13501 gaattaactt aactgtgaat catgatgttt ttaacatcct gataagagct aaaagtttta
    13561 acgctacgtt gttcgatggt caacgcagcg aaaaacaatt cagattttct ttataagaat
    13621 agctgatatt tattaagtta atattaagca gtgatattta gtctataaga taattggcaa
    13681 aaaaaagccc ggtacaatat acgccgggcc tcaattttat tgttgattaa atcagattaa
    13741 tccagccatt cggtatggaa cacaccttct ttatcaatgc gcttataagt atgcgcaccg
    13801 aaatagtcac gctgtgcctg gatcaggttc gcaggcagaa cagcggcgcg gtagctgtca
    13861 taataggcaa ccgcagcggc gaaggtcgga accgggatac cgttctgtac cgcataagcg
    13921 acgacatcgc gcagcgcctg ctggtagtca tcggcaattt gcttgaagta aggagccagc
    13981 agcaggttag cgatctgcgg attttcggca taagcatcgg tgattttctg caggaactgc
    14041 gcacggatga tgcagccagc acggaaaatc ttcgcgattt cgccgtagtt cagatcccag
    14101 ttgtactctt cagacgccgc acgcagttga gagaacccct gagcgtaaga aacgattttg
    14161 cccagataca gtgcacggcg aactttttcg atgaactcag ccttgtcgcc agctggctgc
    14221 gcttgtgggc cagagagaac tttagacgca gcaacgcgct gatctttcag agaagagatg
    14281 tatcgtgcaa acacagactc ggtaatcagc gacagcggtt cgccgagatc cagtgcgctc
    14341 tggctggtcc atttgcccgt acctttgttt gccgcttcat ccaggatcac atcaaccagg
    14401 tagttaccgt cttcatcttt tttagtgaag atgtctttgg taatgtcgat caggtagctg
    14461 ctcagttcac cgttattcca ctcggtaaag atctgcgcca gttcttcgtt ggtgaggttc
    14521 agaccacctt taagcagaga ataggcttca gcaatcagct gcatatcgcc gtattcaata
    14581 ccgttgtgaa ccatcttcac atagtgacct gcgccatcgg caccaatata ggtaacgcat
    14641 ggctcaccgt cttcagccac tgcggcgatt ttggtcagga tcggcgcaac tagttcatag
    14701 gcttctttct gcccaccagg cataatggaa ggacctttta gtgcgccctc ctcaccaccg
    14761 gaaacaccgg taccgatgaa gttaaagcct tctgcagaaa gctcacggtt acgacgaatg
    14821 gtgtcctgga agaaggtatt accaccatca atgatgatgt cacctttatc gaggtatggc
    14881 ttaagggaat caatagcagc atccgtgcct gcacctgctt tcaccattaa caagatgcga
    14941 cgaggcgttt ccagagattc aacaaattct ttcaccgtat agtaaggaac cagtttcttg
    15001 cctggatttt cggcaatcac ttcttccgtc ttttcacggg aacggttgaa aatagagacg
    15061 gtataaccac ggctttcgat gttgagcgca aggttgcgcc ccatcactgc cataccgact
    15121 acgccgatct gttgctttga cattgtttac tcctgtcagg ggttaccgct agatatgcgg
    15181 cttgaataac gtataaaaat gtatcagagt attataaatt tcatgctgta tcagtaataa
    15241 ctaagtaaag gatagcttaa aaagaagcca taatttagta aattagcgaa attatatata
    15301 ttttaaacat tagtcccatt tggaacgtgt tttcgcattc ccattaaata gatgttggcg
    15361 atcttttcat ttctcttgct ggatttccgc acacagttat accggcagga acacttttag
    15421 tgacaacagc ccccatacct ataatggctc ccgcgccaat aataagtggg cgattaggaa
    15481 caccctgctt aattactgca cccgagccta tatatgcatt gtcttcaata acaacatatc
    15541 cattacattt agccccagga gcaaatgtaa catagtctcc tatttgacaa tcatgtgcaa
    15601 cgtatgagta tatgtttgca tgaaaaaacc tccctatatg agtattagta gatattgtaa
    15661 caaagggaga aataatagcg ccactaccta tcatagtatg atcataaaca acgctatttg
    15721 gatgttttat agttattgg
//
LOCUS       DAZFKY010000107        15517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFKY010000107 DAZFKY010000000
VERSION     DAZFKY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            177..608
                     /gene="osmC"
                     /locus_tag="ACHKCL_003033"
     CDS             177..608
                     /gene="osmC"
                     /locus_tag="ACHKCL_003033"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5763956.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(664..1590)
                     /locus_tag="ACHKCL_003034"
     CDS             complement(664..1590)
                     /locus_tag="ACHKCL_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5763957.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(1583..2569)
                     /locus_tag="ACHKCL_003035"
     CDS             complement(1583..2569)
                     /locus_tag="ACHKCL_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5763958.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2566..3462)
                     /gene="ddpC"
                     /locus_tag="ACHKCL_003036"
     CDS             complement(2566..3462)
                     /gene="ddpC"
                     /locus_tag="ACHKCL_003036"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5763959.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3459..4480)
                     /locus_tag="ACHKCL_003037"
                     /pseudo
     CDS             complement(3459..4480)
                     /locus_tag="ACHKCL_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4482..6032)
                     /locus_tag="ACHKCL_003038"
     CDS             complement(4482..6032)
                     /locus_tag="ACHKCL_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5763960.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6046..6627)
                     /gene="ddpX"
                     /locus_tag="ACHKCL_003039"
     CDS             complement(6046..6627)
                     /gene="ddpX"
                     /locus_tag="ACHKCL_003039"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5763961.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(6885..9274)
                     /gene="dosP"
                     /locus_tag="ACHKCL_003040"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             complement(6885..9274)
                     /gene="dosP"
                     /locus_tag="ACHKCL_003040"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            complement(9299..10681)
                     /gene="dosC"
                     /locus_tag="ACHKCL_003041"
     CDS             complement(9299..10681)
                     /gene="dosC"
                     /locus_tag="ACHKCL_003041"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO5763962.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(11058..12377)
                     /locus_tag="ACHKCL_003042"
     CDS             complement(11058..12377)
                     /locus_tag="ACHKCL_003042"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5763963.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(12508..14043)
                     /gene="gadC"
                     /locus_tag="ACHKCL_003043"
     CDS             complement(12508..14043)
                     /gene="gadC"
                     /locus_tag="ACHKCL_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5763964.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(14199..>15517)
                     /locus_tag="ACHKCL_003044"
     CDS             complement(14199..>15517)
                     /locus_tag="ACHKCL_003044"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5763965.1"
                     /translation="SKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENV
                     HKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEAC
                     MLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLF
                     MDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAAS
                     GGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVD
                     YLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGP
                     YEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVV
                     MRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         3878 a         3967 c         3888 g         3784 t
ORIGIN      
        1 gattaatatt gatgcaatct ctcttgagat atatatttcc ggttatctat tgtgggaatt
       61 taatttaagt gcagaagtaa tatttttgcc gtattttatt cggaatatcc tgcttatcct
      121 cgtgcggttt ctcacgtagt ctataatttc ctttttaagc ccacaggaga gcaacaatga
      181 caatccataa gaaaggtcag gcacactggg aaagcgatat caaacgcggg aagggaacag
      241 tatccaccga gagtggcgtg ctgaaccaac agccgtatgg atttaacacg cgttttgaag
      301 gcgaaaaagg aaccaaccct gaagaactga ttggcgcagc gcatgccgca tgtttctcaa
      361 tggcgctttc attaatgctg ggggaagcgg gattcacgcc aacatcgatt gataccaccg
      421 ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc gcactgaaga
      481 gtgaagttgc ggtgccgggt attgatgcct ctacctttga cggcatcatc cagaaagcaa
      541 aagcaggatg cccggtctct caggtactga aagcggaaat tacgctggat taccagttga
      601 aatcgtaaag cattgccgga tgacgcgtca ggcgcatgaa tgcctgatgc gttgttagca
      661 tctctacagc gcccgccagc aacggatctc acgtccgtca gcccgcgtcg ttaatgattg
      721 ccggacttcg catccgctgg ttgcaagggg gcaacgttcg cggaaaaaac agccctgtgg
      781 caacgtgcgg tttcctggca gatccgtttt acgtaatgcc cattcttcct ccagtggttt
      841 atcaatggcg gggagggaat ccagcaataa tcgggtgtat ggatgtgccg gagccgttag
      901 cacctgttgc gcttccccta attccacaat ctgcccgaga tacatcaccg ccacccgatc
      961 gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataagtca gcccgtgatt
     1021 ttcctgtagc gttaccagta aattgaggat ctgcgcctgc acggagatat ccagcgcaga
     1081 ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg caatggcgat
     1141 gcgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact ccggacgaat
     1201 acccacctgc acagccagct cctctgccaa cgctcgccgc tgttgttcac tactatgctt
     1261 agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat taagcgaaga
     1321 gagcgggtcc tgaaacatca tctgcataat gcgttgtgag ccagaacgga tgtactgccc
     1381 gtggctcggt tgcagcatac ccattaaaag ctgtgcgagg gtgcttttgc cgcagcctga
     1441 ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac cattaatggc
     1501 atgaacatgt tccgtagttt taccaagcca gtttttacgc gccgggaaat tgatatggac
     1561 gtcgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga taccaacagg
     1621 cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca cactgtgcac
     1681 ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg agatttggta
     1741 ccgtccctgg aatggcgggt aatggctggc gtggtactcc atgttctggt gcgcattgca
     1801 gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccgtc acgccgctct
     1861 caatcacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac accacggcca
     1921 tatcatggct gatgaacagt accgcagttc cactgtcgcg agctttatgt ttaagcaaac
     1981 gcagtacctg caactgtacc gtgacgtcca gcgccgtagt cggttcgtcg gcaataatca
     2041 attgcggttc gcaggagaat gccagcgcaa tcattacccg ctggcgcata ccacctgaaa
     2101 gctcaaacgg atagcgcgac ataacttcca cggtatccgg gatttgcatc tcttccagca
     2161 gggcaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg cggatcacgt
     2221 ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt tcctgaaaga
     2281 tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgcgcg ttaagaacat
     2341 cgtctcccag cagtgaaatc tgcccccgat gtacgcaata actgcccgtc ggtagcagac
     2401 gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc agaccgacaa
     2461 tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacgtcg ccgttaaaac
     2521 cggggaaact caaatgcagt tgttgaatgt ccagaacggg ttgggtcatg actgctttcc
     2581 tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa acccgacggc
     2641 ggtaagcaaa atcgctgccc ccggaaatgc gcaataccac cactgatcga gtacatagtt
     2701 gcgaccattt gctaccatcg cgccccattc agcactcggt tgttgagcac ccaggccaat
     2761 aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg cctgcacgat
     2821 cagcggcggt agggagttac gtaaaatatg ccagttgatc agatgccaac gagacgcgcc
     2881 aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt gcccccgcgc
     2941 caggcgcaca taaaagggaa ttcgcacaat agcaatagcc agcatggcgt taaacaaact
     3001 cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg acagcataat
     3061 gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg atagacatcc
     3121 gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga gaattgattg
     3181 ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg tgccaaacca
     3241 gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg gatcatgcgt
     3301 cgccagccac ggtgaaaaaa tcatcatcag tagcattaat acaatgatga ctgcgccggt
     3361 cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa atcgcgtttg
     3421 tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc tccacgtccg
     3481 atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag cacataagca
     3541 aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg tattgatgtc
     3601 actacccatg cccccattcc gggccaggca aaaacggttt cggtgagcac tgcgccatac
     3661 aacaaatcgc ccagcgccag ccccaatacg gtaatcgaag ggatcaacgc attgggtagc
     3721 gcataacata gaacgatata ccagccgggc aagccgctgg cccgggcggt acgaatgtag
     3781 tcttcactca attgttccag catcgctgag cggatctggc gggcgacaat ccccaggtga
     3841 acaaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa gaagacttca
     3901 ccgttgcctt caagcaacgc atcgagcaga taaaagccgg taacgtgcgt tggtggatcc
     3961 agccagtcat caagccttcc gccgccggga agtatttgca gatgaccata aaacagcacg
     4021 atgacgccca gcccaagcca gaacgcaggt gtggagattc cggtaatcgc cattattcgc
     4081 accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag cgggatgcca
     4141 atcaagagtg ccagcagcag ggcacaaaaa gccagctcca gcgtcgccgg gaaaaatata
     4201 cgcaactctt ccagcaccgg acgcccagta cgaatggatg ttcccaggtc accatgaaac
     4261 aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc cagttgctgg
     4321 cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg cgccggatcg
     4381 ccggggatca gatgcgagat aataaacgta atcacgcaga cgcccgccac cacgagcacc
     4441 agccccagca gcgttggcgt aaaatactcc agaaggtcac gttatttact catggtattg
     4501 atattgaaga cctgttccag catgggattg aacacaaagc ctttcacctc tttgttcatc
     4561 gccagttggt agtttttctg gaacaggtac acataagcag cgtcatcaat gacgattttc
     4621 tgtgcctgct ggtagtctcg ggtacgctgc gtctggtcgg tggtcgcaag cgcattgcgc
     4681 agtaacttat cgacctcact gttttcatag aacgagcggt tacccggcag accttttttg
     4741 tctgactcaa accagtaatt cataaacata tacgggtcgg caaaatccgg actccagttg
     4801 ccaatcgcaa tgtcgtaatc acctttaccc actctgtcgc gcatggtggc gttcgccagc
     4861 ttttccagct tcacattgat gcccagcttg ttgaggctgg attgtgttgc cagagcgatc
     4921 ggttcccagt tcggatcgtt atcggagtag agaaacgtca ggctggtggg tttgctcgtc
     4981 actttatccc attcggcttt ggctttcgtt tcgtcatggt tgtattgcat tgccgtcgca
     5041 tcgtagcccc acatgccttc cggaatcggg ccgcgcattt gttttccgtt accactcaga
     5101 atgccgttaa ccatgccctg ataatcggta gaccaggaaa tggcccgccg cagatccgcc
     5161 tgattaagtg gcgctttgct gttattcaga tacagatagg tgacgcgcag tgacggatac
     5221 tctgccacat tgactttgtt ttcctgcttc agggcgttga gttgatccac cggcagcgca
     5281 tcggcaatgt caatgtcgcc acgggagagc tgcaggcgac gggaggcact ttcaccgata
     5341 atttttaccg atactcgctt aaagttcggt ttattgccgg ggtaatgcgg atttggcacc
     5401 agaactaatt gctgaccttt ttgccagctt ttcagcataa atggtccgga accggcggta
     5461 ttttgcgcga ggaagccgcg ggcatcatcc gccgcatgtt cctttaatac cgccggattg
     5521 ataatggatg caccgtcatt cgccagcgtg tagaggaacg gtgcaaatgg ctggctaagg
     5581 gtaaacttca ccgtatgttc gtcgggagca tcaatcttta aatctttggg aaatgcttct
     5641 gctggcccct ggccgatttt tagtaaccgc tcaaaagaga gttttactgc ttcggcagtg
     5701 acaggtgtgc catcggcaaa tttagcatta tctttcaggg tgaacgtcca ctctttttga
     5761 tcgttagacg ctttccagct acttgccaga tcgccttcaa cgtcggttga gcctttatca
     5821 ccgtccgttt tgtactgaac cagtcgctga taagacgggt aggtcactgt ccagtcgtta
     5881 ttatctattg ttaccgccgg gtcgagggtt tgtggatcgg cggccttacc aatcaccagc
     5941 atatctttcg gtacggcggc gtgcgcaacc gggaaagttg tggcaaggac gagcgcgagc
     6001 aatgtgggac gaaacgatat cgatctcttc atggcagttt cctgattaac tgacgtgctg
     6061 tgtgccaggg gatataaaac aggtgaattg atcagcgagc agagggtaac tcgctgcctg
     6121 gggtaattcg aagtgccacc attcgctgga gataccgaca aaaccgccac cagtcattat
     6181 cgcattcagc aacaggcgat tgcgctgagc ggcgggcggg acggaagggt gataggcatg
     6241 ggagcgctcg tgcatttcat cgaaccctgc gcccatatcc aggatgttcc cgtgctcatc
     6301 acgaagcgtc aggtcgatcg ccgtgccacg gctgtgatta gaaccgaccg tcacatcaac
     6361 aacatattgc gggtctgggc aggcttgcca caacatcgcc tgtgcttgtt gtgggcgata
     6421 cgcatcgtaa atcaccagtt gtaagcctga tagctgggcg atactgatac ttttcgccag
     6481 cgcggtaatc gcatccttgt gtaacagaca acgcgcttgc tggtaaatag ctttacctgt
     6541 gatgttatca gcgcaggcgt atttcaattc gatctccaga tcagggaaga tcacggctaa
     6601 atcaaccagt tcggtggtat ccgacataaa cggttcctgt ataagacaaa aattgctgcg
     6661 ctttccgctt atgcagatct catgccatgc cgggataagc gccagaatgc tggcttaaag
     6721 ttatattttt taacttttga tcaacatttg tgcagcgtag tgcagttttg gtgcaagagg
     6781 ggaagttaag gaaggaatct cccggaatcg tagctgaaat cacagtattt aagtgacagt
     6841 gtcacgttaa atgaaaaccc gcgagtgcgg gagagaggaa tttgtcagat tttcagcggt
     6901 aacacgctgc tcatccagcc tggaatttct tcggcgggta gggggcggga aaagaaatat
     6961 ccctgaataa cgcgacagtg gatctttcgt agcatctcaa attgctcttt ggtttcgacg
     7021 ccttccgcca cgacggttaa attgaggctt tgcccaatgc tggtaatggc ttcaagtaag
     7081 gccaggatgc gtttttccgt cagacaacga tcgacaaaac ttttgtcaat tttgatttcc
     7141 gttaccggaa gactgactaa gcgggataat ccggaaaagc ccgtgccaaa atcatctacc
     7201 gataaaccca cgcccatatc acgcaggatc tgaatgcgct taaagatttc ggtatcgtgt
     7261 tccatcatca tgctttccgt gatttctacc gtcagctggt ggccgtcaat accccaggct
     7321 tgcattgcat cagacacctg attaggcagc tgattactgc gaaagtgcag cgccgacaag
     7381 ttcacggata acgccgggat atgaatattc tggctacgcc attctgctaa ctgacggcaa
     7441 gcttccgcga tgacccagcg cccaatattt tcgatttcac caatctcttc tgcgagagga
     7501 ataaaccgtg aagggggcac atgaccatgc tggggatcgt gccagcgagc aagggcttcg
     7561 atgccgtaga gttcacccgt ttctgcgaag atttgcggct ggtaaaccag tttcagttgg
     7621 ttattgctaa tcgcttcttt cagcgctgcg cctaaaacca aacgctcttt taccatttcg
     7681 ttcatcgccg ggctgaagaa ctgccagccg ttaccgccat tcttgcgaat ataatccatc
     7741 gcgttatggg cagtggagag caggtaatcg cgatttttac ccacgtcgta gctgatgcca
     7801 atactcaagg taagcgggaa ggggttatcg tcaatcatta tcggcttgct gaccacattc
     7861 cgtagctcat cggcgatctg ggtaatgtta ctgacgtcgt tttccaggct cacgaggaca
     7921 aactgcgtac cttcgatacg acagagatac tgatccggtt tgagtttttc acgaaagcga
     7981 ttgaccactt ccagcaatgc ctgatcggcc cacgcatagc caaggctatc aatcacatcc
     8041 tgaatatggt caacaccgat actggagaga cggctttgtc gaccaggtca tcgaggtaat
     8101 tgtgcaggtt attgcgattt ggcagaccgg tcatcggatc aaattggatg agttgttcaa
     8161 tatgctgacg gcttttttcc tgttccagcg ccagcgcggc catatgctgg ctgatatctg
     8221 ccacgcgttc gataaaggcg ctggtttctg ctcctgacga ggttttaatt tgcaggatcc
     8281 ccgcaggcgc gccatcacgc tgacgaatgg tcgctgacca gctttgcgca ttttgaactt
     8341 ctgcaccgtg cgaagatgac gcccagtgta tcggcatccc gttgcgcagt gcgaacagcg
     8401 aaacatgcga ttcgttgaga acagattcga tgttacgaca aatgatttcc cccatttcat
     8461 gaaatggcgg gctgctgcac atggcggcga gaatattgcc ttcaagctgg cggatttgcc
     8521 gttcttcggt gatatccgag aaggtcatta ccaggttctg cagatgcgcg agcacgtcat
     8581 aaaccgggct gatagaagct ttaatccaga ttttttcacc ggtgcgcgtc aacagcagaa
     8641 attcgtcctg atcgcgggcg gttttccaaa gcaactgttg taaacgaatg cggttatcgg
     8701 cagggaattc aggaatgttc aggagtgtat caggctgcat accgctggct tcgctaatgc
     8761 agtaaccaaa catttcggta aatgcgcgat tgcactgcac aatatggcgt tccggatcga
     8821 ggacaatcac cggtcggtcg agatggtcaa cggcaataat caattgcctg gtctgttctt
     8881 tttgcgccat ttctacgctg gcatcccgta ccagcgccag gtaataaact ttcccctcgg
     8941 cgctcacttt cgatagcgca aaacgggtcc agattttact gccgtctttt ttctccagct
     9001 gcagctcccg actcatcccc tcgacgcgcg ctttaccgcc ttcacggttg tgacgaatgt
     9061 attcaggatg cgcaggacgc aaatcccgcg gaatcagcat atcaatgtta ttgccaatga
     9121 cttcttcacg tttgtacccc cagagcttct ctgcggcggg gttgaaaaac atcacttcat
     9181 cattttcgtt aattaacacc gcacccatca tattttgctc aagggcgggg aaaaaaatgc
     9241 catcggcggc attatccgca tcggttagct tcatgattac ctctgcatcc tggcgcatct
     9301 aaagactggc tttccagagt tcaacacggt ttctacctcg tcttttggcg atatacagag
     9361 cttcatcggc tatttgaatg aggcgctcat agtcaggatg accattaaac atggcggcac
     9421 cgatggaaag tgagagggca atatcttcgc cgtttgcggc tttcagtttg gttttctcca
     9481 cccgactgcg aatacgttct gcggtacgta acgtttcgtt ttcagaagct tcagtcaaaa
     9541 caatgataaa ttcatcgccc ccgtagcgga aaacataatc actactgcgg acgttgtcat
     9601 aaaaggcctg agagacttta cgcagaattt catcgccagt gttatggccc cacgtatcgt
     9661 tgatctcttt gaatttatca acgtcaataa tcagcactga cagcggtgta ccggtccggt
     9721 tggcatgggc aatttcgcgt ttgaagatag tcggcaggaa acggcggtta agtaatttcg
     9781 tcagtacatc cataccgact tcgtggcgcg atacttcttc aaacaattca cgcagcaagg
     9841 taataatttg cgatacggta tttcttatct gtaataaaaa tttcacccgc agacttctgt
     9901 tattcaaatt cctggtgtta cgcatggttt gattgaaaat accgtcgaaa tcctgaatca
     9961 gacgagagat atggcccact tcagcaatgc cgctgaaata atgtcgacct ttatggttaa
    10021 accacaggcc aaaatcagcc tggcttaaag gcaaactact gcctaaatca gaatccagca
    10081 ggattttata gataatatct atttcccatg aaagtattga ggctatttgc cgttcttttt
    10141 cttcttcggc gttttccagt aacgagaaga tacgatagtt ttcatcttct tttgaggcac
    10201 tactgtcact aaaggtaaac gcgcgggtca tcacttccat agcgatatca atactgttaa
    10261 tcgagaaatg gtagacctga agtttttctg cggcagaata atccgaagag aagattaccg
    10321 gatagaggat ctttttcagc acccgaaacc ccatctcgac aatttctacc ggaattccta
    10381 tgcgggcatg cacttccgcg acggtatgct ggatttgtat tagcctttcg acatcgtcaa
    10441 cctgggcaga aagcacgtta ataatccagc gttccatcgc acttttcaac tgccgctcaa
    10501 cttgttcatt actcaagaat tcttcggcat gcgggtcgat gcggacaatt cgataaaact
    10561 cgatactcag ataatgagca tgcgcaacgg caatttccgc ggctttagca cgaatgggcg
    10621 gatctgcctg ctcgacaagt ccggtccact catctttcat tcttttaaaa tacatctcca
    10681 taattcacac ccttataagg ctgggaaatc agacggaatc aaaatgaaac gcgacgtgcg
    10741 agatcgacta actgcaccat attctcctga aatatgaaga tatactgaaa agaaataagc
    10801 gacttaggac agtttcaatc tacgctactg ttcttcagaa gagtatagcc catcgtaatt
    10861 atttttcggt gactgcgaat atcgtatggt ttttcatatt catacatttt tattagggga
    10921 tttatggctg tttaactaag cgtggttaat tgcgtggtta atttgacttt agtaagcatg
    10981 attgctggtg ggatagttca agagggtaac aagccagtgg gtaaagcacc ggcttgttac
    11041 aaagtaagaa tgggagttta actgccccaa cgactttgca gatagctgac cgcttgttga
    11101 gtctgcggtt tattcagata gtcctcacgg aacaagatgg tgccgctaat ttctggtacc
    11161 gcatcgttaa gatcgagctg ctttttcagc tccggtacgc cgccgttaat catccagtct
    11221 ggctctatct ttgaaggttc acccacttta tagaaggcga taccgatata caggcgggtc
    11281 ctggtcggtt taacgacatc cgcccaccat tttgccaaca cgtcataacg cgcggcactc
    11341 cgtgagaacg gccagtaaat ttggggagca atgtaatcca gcaatccttg ttccacccat
    11401 cgacgagtgt cagcgtagga ttcgtcatag gctgccgcgc ctcgggtatc ggaaccgagc
    11461 ggatcgtgtg atcggttacg ccacacgcct gccgggctaa caccaaattc gactcccggc
    11521 ttaatgcttt taatggtgtg cgataccttt gcaattaact gctgagtatt gttgcgccgc
    11581 cagtctgctt ttgacgcaaa tgcgcctccg tatttacggt acgtttcgtt atcatttagc
    11641 cgtgaacccg gtgactcagt atagaaatag tcgtcaaact gcacgccatc taccggatag
    11701 cgggaaacca cttctgcgac tattgatgtg atccagtcct gaacctcagg gatgcccggg
    11761 tcgaggacaa agcgatcgcc agacgttctg atccagtcac ggtgttgcac atagacgctc
    11821 gccggttgtt gagacagagt gctattcagt tccctgatag taccgggctt cgtattaacc
    11881 gatacgcgat aggggttaaa ccaggcgtgt actttcatcc cacgcttgtg ggcttcgtcg
    11941 agcataaatt gcagcggatc gtaacccgga ttttcaccaa tcttaccggt cataagatcg
    12001 gaccacggca aaattttcga tggccacagg gcggtaccgt ccggcttgac ctggaaaaag
    12061 accgtgttta taccgagacg ttgcaaatga tccagtttgt cgatcatcgc ctgttgttgt
    12121 acacgggccc ggctggtggg gttactaatg ttaaccgagg aaaccggtgg ccagtcgagg
    12181 cgagacaccg tggccagcca gatgccacgc atcggttgtg acgattgttg cgtcgtggct
    12241 ggtggctttg aacccgctgg tggtgtcacc atggactctg gaggcgtgct tttacaacta
    12301 cacagtaaaa gtgccagtgc aaccagtatc gctggtcttc taatcgttaa tttcttgttt
    12361 cgggagcaga tatccatatg tgctagtttc cggtaaacag atgtgcgctc attctcgtac
    12421 ttatcccggt taagtcaata cgacagcaag cacgaaaaag ggagcgatga attatcgctc
    12481 ccttatctta taaccattca gacatagtta gtgtttcttg tcattcatca caatatagtg
    12541 tggtgaacgt gcacgcgggt gcaggaagaa gtgacctttt ggtgcgttct gactgttgat
    12601 tggctccaga gtgacgccgg tattcgcttt gcctttacga tcatgaacag catagagaat
    12661 aaagggcagg gcaagtacca ccaggaaact aacgaccagt aattcaacat acatatcggt
    12721 agagtcaccc tggatgttat ccggcggcag gaaggaaaca ataaacgcca taattgaagt
    12781 cagcagaccg acaattgcca cgaccagttt caccccttta ccaccaggga tattaaatgt
    12841 gcgttttaag tcaggatgtt taagaaccaa cacaatgtag ccaataaaca gcatgaaata
    12901 agcacacaga taaatcacca ccgtcagcgc cagtgcgatc aggaaggaca tgttgttacc
    12961 gccaccggta ttggtgagga tgatcaacgc gatagaagta atcaccagct gcgaaatgac
    13021 cagcgttacc ggtacgccat ttttgttcat tttagcgaat gccgctggca gcaggttttt
    13081 ctgcgctgtg acatacatcc cgcgagaagg accaacaatc caggaggcga tttccgccag
    13141 aacacccagc aacagcagtg cggagatcac gcgaaccgtc cactcaattt ccggtgccac
    13201 atgggacatc agaacggtaa aggtttgcat tacccctgcg gagaggttga tttcattacc
    13261 cggaatgacc atcgcaatag acaaaccgcc aacagagctt aagcagattg ccgccaccat
    13321 cagcagtaac atagccagtg gatagtcgcg ccctgggtta ctcatttcat tgacgtgggt
    13381 tgcggaagct tctacgccca tataactcaa aatgaaggca acaaatacaa ccagggtgcc
    13441 cactttagag aagtcaggga agaaggtctt cgaatccatt tcgatagcaa cgggggcacc
    13501 ggagtgcaga taaatagccg ctaatgcaat caaaataaat gcaggtaaca ggataccggc
    13561 gaagaagcca actttagcaa ttcgcgccgt gtatttcgtg ccaccaaact gcgttaatgc
    13621 cagcgcccaa agaatgatga gtgctgcaat agttttggta atggggtctt cattcagcgc
    13681 tggccatttc aggatgtagg agagtgcccc taacacgaaa tagagcatcg gaataaaacc
    13741 aatggcgatt tgcagatagc caaatgagat cgctgcaaat ccccatctcg gccccagagt
    13801 atttgatacc caggcgaaga caccaccttc ttcccagccg tcgacggtgg ccatttccgc
    13861 agcacaaagt cccacgggaa taaaccataa aatcccgcct aatagcagga agaagactaa
    13921 tgaaaagccc gatgttgcga aggtagggta ttcataaaca gccattacca tcgatgccgt
    13981 tatggcaaaa aagccgagta atgtgagctg cttagcttta cctgtctgta ctaatgtagc
    14041 catattatcc ccctaaaacg gtattcctgt cggaaccgca cctgttttgt tctgaagcgt
    14101 attcagaaca atattttccg ttgctaatgc cagtgaacag actttggaaa ttgtcccgaa
    14161 acgggttcgt ttcgggacac cgttaccgtt aaacgttatc aggtatgttt aaagctgttc
    14221 tgttgggcaa taccctgcag tttcgggtga tcactgagat atttcaggga ggctttgtag
    14281 tcttccagca acagttcagc aaagtccatt tcgaagccgc gacgacacat aatgcgcatc
    14341 accacgatgt cggtggcttc accgccgaga gtgaaggccg gaacctgcca gccgcgcaga
    14401 cgcagacgtt cagagaggtc atacagggtg tatcccggat cttcaccatc tttcagtttg
    14461 aagcaaaccg ccggaatgcc ttcgtccggg cgaccggtac agatgaactc atacggcccc
    14521 agtttggcga tttcatccgc cagataagcg gcaacctggt aagaggcgtt ctgtactttg
    14581 gtatagcctt cacgaccgag gcgcaggaat tcatagtact gtgcaattac ctgacccgcc
    14641 gggcgggaga agttgatggc gaaagtacca atctgaccac cgaggtagtc aacgttgaac
    14701 accagttcct gcggcagcgc ttcttcatca cgccagataa cccagccgca gcccagcgga
    14761 gccagaccga atttgtggcc tgaagcactg atcgatttca cacgcggcag gcggaagtcc
    14821 cagacgatat ccggggcgac gaacggtgcc aggaagccac cactggcggc gtcgatgtgc
    14881 atgtcgatgt cgataccggt gtcggcctgg aatttatcca gcgcatcgtg cagcggttgc
    14941 gggaactcat agttaccggt gtaggtcacg ccgaaagtcg gcaccacgcc gatggtattt
    15001 tcgtcgcagg cttcaatcat gcgtttcgga tccataaaca actgaccggg gcgcataggg
    15061 atctcacgca gctccacatc ccagtagcgg gcgaatttat gccagcagat ttgcaccggg
    15121 ccgcacacca ggtttggttt atccgttggt ttgcctgcag cttccatacg cttgcgccaa
    15181 cgccatttca tcgccatccc gccgagcata caggcctcgg aagaaccaat ggtgttggtg
    15241 ccaacggcct gaccattttt cggcgcaggc gcatgccaca gatcggcaac catatttacg
    15301 caacgcaggt cgatggctgc agattgcgga tattcttctt tgtcgatcca gtttttgtta
    15361 atggataaat ccatcaactt gtggacgttt tcgtcgtccc aggtctggca gaaagtggcc
    15421 aggttctgac gagcgttgcc atcaagatat aattcgtcat tgataatctg aaatgcgaca
    15481 tcgtcgcgca tttcgtgcag cggaaaacgt tttgatt
//
LOCUS       DAZFKY010000108        15458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFKY010000108 DAZFKY010000000
VERSION     DAZFKY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            125..328
                     /locus_tag="ACHKCL_003045"
     CDS             125..328
                     /locus_tag="ACHKCL_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5763966.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHKCL_003046"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHKCL_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHKCL_003047"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHKCL_003047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKCL_003048"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHKCL_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5763967.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHKCL_003049"
     tRNA            2484..2559
                     /locus_tag="ACHKCL_003049"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..10855
                     /locus_tag="ACHKCL_003050"
     CDS             2873..10855
                     /locus_tag="ACHKCL_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO5763968.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11117..12169)
                     /locus_tag="ACHKCL_003051"
     CDS             complement(11117..12169)
                     /locus_tag="ACHKCL_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5763969.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12483..13799
                     /gene="shiA"
                     /locus_tag="ACHKCL_003052"
     CDS             12483..13799
                     /gene="shiA"
                     /locus_tag="ACHKCL_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5763970.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13901..15355
                     /gene="amn"
                     /locus_tag="ACHKCL_003053"
     CDS             13901..15355
                     /gene="amn"
                     /locus_tag="ACHKCL_003053"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5763971.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4228 a         3680 c         3713 g         3837 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc aaacatcgaa agcggaaatc attgggaatg gcgtggatga
     6661 gacgactctg acggcaacag tgaaagatcc ttttgataac gcagtaaaag atctacaggt
     6721 caccttcagt accaaccccg cagatactca acttagtcag agcaaaagca atactaacga
     6781 cagtggtgtg gccgaagtta cctttaaggg cacggttttg ggtgttcata cagccgaagc
     6841 cacactgcct aacggcaaca acgatacgaa gatagtcaat attgcgcccg atgccagcaa
     6901 cgcgcaggtt acgctgaaca tccctgctca acaggtggtg acgaataaca gcgacagcgt
     6961 gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa taacggtgaa
     7021 cttcaccatg ccacaggacg tggcggcaaa ctttaccctc gaaaataacg gtattgccat
     7081 cacccaggcc aatggggaag cgcatgtcac gctcaaaggt aaaaaagcgg gtacgcatac
     7141 ggttaccgca acgctgagta ataacaatac cagtgattca cagccggtaa cgtttgtggc
     7201 ggacaaaacc tcggctctgg ttgttcttca gatatcaaaa aatgagatca caggtaatgg
     7261 cgtcgatagc gcaacgctaa ctgcaacggt caaagatcag ttcgacaatg aggtgaacaa
     7321 tcttccggta acattcagca cagcttcttc aggcctcacc ctgaccccag gggaaagtaa
     7381 taccaatgag tctggcatcg cgcaggccac tctcgcaggc gttgcctttg gtgagcagac
     7441 ggtcactgca tcactggcta ataatggtgc cagcgacaac aaaactgtgc attttattgg
     7501 cgacacagcg gcggcaaaaa ttatcgagtt gacgcctgtc ccagacagca taatcgcagg
     7561 taccccgcag aacagctccg gcagcgtcat caccgccaca gtcgttgata ataatggctt
     7621 tccggtgaaa ggtgtgactg tgaacttcac cagcaacgca gcgacagccg aaatgacgaa
     7681 tggcggtcaa gccgtgacga acgaacaggg taaggctacc gtcacttata ccaatacccg
     7741 ctcctcgata gaatcaggag cgagaccgga taccgttgag gccagtctgg aaaatggtag
     7801 ctccacgctt agcacatcaa ttaatgtcaa cgctgatgcg tctacggcac atctcacctt
     7861 gctacaggca ctttttgata cagtctccgc aggcgacact accaatctgt atattgaggt
     7921 gaaggataat tacggcaacg gagtacccca gcaggaggta accctcagcg tttcaccaag
     7981 tgaaggtgtg acccccagta ataacgctat atatacgacc aatcacgacg gcaattttta
     8041 cgcaagcttt accgctacaa aagccggggt ataccaagtg acggcaaccc tcgaaaatgg
     8101 cgattcgatg caacaaacag tgacctatgt gccgaacgta gcgaatgctg aaatctcgct
     8161 ggcagcctcg aaggatccgg taattgccaa caataacgat ctcacgacac taacagcaac
     8221 agtcgctgat acagagggca atgcgatagc caacagtgag gtaacattta ctctgccgga
     8281 agatgtgagg gcgaacttca cgctgggcga tggcggtaaa gtggttactg atactgaagg
     8341 caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct catactgtta cagcatcgat
     8401 ggctggcggt aagagtgagc agttggtggt gaactttatt gcggatacac tcactgcgca
     8461 ggttaatctt aacgttaccg aggacaattt tatcgctaat aacgtcggga tgaccaggct
     8521 gcaggcaaca gtgactgatg gaaacggcaa cccgttagcc aatgaggcgg tgacattcac
     8581 gctaccggca gatgtgagcg caagctttac tctcggacaa ggcggttccg ccattactga
     8641 catcaacggc aaggctgaag ttacactgag cggtacaaaa tccggcacct accccgtgac
     8701 agttagcgtg aacaattatg gtgtcagtga tacgaaacag gtgactttga ttgccgatgc
     8761 tggtaccgca aaactagcct ccttaacctc tgtatactca ttcgtcgtca gcacgaccga
     8821 gggcgcgacc atgactgcaa gcgtcactga cgctaacggc aacccggtag aaggtataaa
     8881 agttaatttc cgcggaactt ccgtcacgct aagcagcacc agcgttgaaa cggatgatcg
     8941 gggtttcgct gaaattcttg tgacaagcac cgaggtcgga ctgaaaacag tttcagcctc
     9001 tctggcagat aaacctactg aagtcatctc gcgattactg aatgcaaaag cagatattaa
     9061 ttctgcaacg attaccagtc tggagatacc tgaaggtcag gtcatggtcg cacaagacgt
     9121 agcagttaaa gctcacgtca acgaccagtt tggcaatccg attcttaatg aatctgtaac
     9181 attcagtgca gaaccaccag agcacatgac catcagccaa aatattgtct ctactgatac
     9241 gcatggtata gccgaggtca ctatgacgcc cgaaagaaac ggttcgtata tggtgaaagc
     9301 atccctggcg aatggatcct cttatgagaa ggatctggtg gtaatcgatc aaaaactgac
     9361 actctcggcg tccagcccgc ttatcggtgt caattcccca acaggtgcaa ctctgacggc
     9421 aacgctaact tctgcaaatg gcactccagt ggagggtcag gtcatcaact ttagcgtaac
     9481 gccagaaggt gcgacgttaa gtggcggaaa agtgagaacc aactcttcag gtcaggctcc
     9541 agtcgttctg accagcaata aagtcggtac atatacggtg actgcatcgt tccataacgg
     9601 cgtaacaata cagacacaga caatcgtgaa agtcactggc aactcaagca ccgcccatgt
     9661 tgctagcttt atcgctgatc catcgactat agccgccacc aacagtgatt taagtacctt
     9721 aaaggcaacg gttgaggatg gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc
     9781 cttaaaaagc ggctctgcca cattaacgtc attaacagcg gtgacagatc aaaacggaat
     9841 cgcgacaaca agcgtgagag gagcgataac ggggagcgtc acggtaagcg cagtcacgac
     9901 cgctggtgga atgcaaacag tagatataac gctggtggca ggcccggcag acgcctcgca
     9961 gtccgtcctt aagaacaatc ggtcatcatt gaaaggagac tttaccgata gtgctgagct
    10021 acatcttgtt ctgcacgata tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt
    10081 tgtgcagtca ggtaccaacg cgccctatgt gcaagttagt gcaattgact acagtaaaaa
    10141 tttctcaggc gagtacaaag ccactgttac aggcggcgga gagggtatcg caacgctgat
    10201 ccctgtattg aatggtgttc atcaagcggg tctgagtacc acaatacaat tcactcgcgc
    10261 agaagacaaa ataatgagcg gtacagtgtt agtcaatggt gctaacctac cgacaactac
    10321 attcccttcg caggggttca ctggggcgta ttatcagttg aataatgaca actttgcccc
    10381 aggaaaaacg gcggctgatt atgagttttc aagctctgcc tcctgggttg atgttgatgc
    10441 taccggtaaa gtgacattta aaaatgtcgg cagcaaatgg gagaggatta cggcgacgcc
    10501 aaaaacaggc ggccctagct atatatacga aatccgagtg aagagttggt gggtgaacgc
    10561 cggcgatgct ttcatgatat acagccttgc tgaaaatttt tgcagtagca atggctacac
    10621 acttcccctt ggagaccatt taaaccatag tcgttcccga ggcatcgggt cactgtacag
    10681 tgaatgggga gatatggggc attacacgac tgaagctggt tttcattcaa atatgtattg
    10741 gtcatcgagt cccgcaaact caaacgaaca atacgtagtt tccctggcaa caggtgatca
    10801 aagcgtattt gaaaagcttg ggtttgctta tgcgacatgt tataaaaacc tctgattttc
    10861 cgttataatt tcttaaagag tgctccattc ggggcactct tttttacatt ccctctatag
    10921 agcttgccct tctctcacag aaaatgaata aataaaaatg cgataccgca agaatcgcgt
    10981 tcgatgtgac tatgatcgta tccggtgagt acagctttta tccaaaggct ggttttctgt
    11041 gatgattgac cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact
    11101 atgcataagt actgtctcat attctcgcca aaacactttt taatgccttt gcgaattcat
    11161 ccagacttat attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg
    11221 cgggccatcg cgttttgtaa tcagcataaa ttgcaacaac gggggtatta taggcacttg
    11281 ccatatgtaa tattgaggtg tcaggagtta ttgcgatata agcatctttt atgattgctg
    11341 ctgaacgttt aatcgagggt gataaggata aacggtagac attattataa caatccacta
    11401 ataccctggc tttatcttcc ccttttggtc catgaacgat cactattggt atatctgttt
    11461 cactttgaat tttttctatt agattttctg caatcgatag tgaaaatgta cgatcctgcg
    11521 agctaccttc taaattaagc gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct
    11581 catcgagtac atcctcactt agtggcaatt catatattgg cctgactgct ggaaaacccg
    11641 cctcacgcat cataaatgcc catgtcatgg gtacgggagc ccggagtttc tgatccatcc
    11701 gggacgcgtt cttacacaca ggggaatagc acttcatggt taaaccaaca acctgaaaat
    11761 tcgtttttgc tttcaactga ctgataaata acatcgtttt caggttcttt ttacgcatcg
    11821 cctctataca aagatccggc gtaccgtatt gctgtgttat gttcttcgct aaatctttta
    11881 tttcttttaa tgttgcgtga tcctgcatag tcattgtgac taatgttaat ttagtctgtt
    11941 caagtttgag cgcattaaag acttctaaat taattgtcga cgtaacaatt aatagatgct
    12001 taattttatg caattcaagc gcccgaataa caggaaagat ggccatagca tcgccaatct
    12061 gatcgggaat atggatgaca acaaagtctg ttttttcaat attgaaatta taagctttat
    12121 aatcgtagta actaaatgca atacgtctca acaatgatac taaaaacata actaacctcg
    12181 cctccctact ggttataatg caatgcagtc tatcagaatc atcagggtgc aattttgtgc
    12241 atatgcggac ttttatgttt catatctcga acctgtgggg cctctgctta atccttaaac
    12301 aacaccagca actcctgcgc tttcatcttc cagcgaattt ttcatgttgc cgttaatcag
    12361 ccataaaatc atttgcagat gcgctctgtc gaggtagtct cataaggttc gtttatagat
    12421 cgacgcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
    12481 ttatggactc cacgctcatc tccactcgtc ccgatgaaag gacggtttcg ttaagtcgcg
    12541 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
    12601 tctatggcat taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
    12661 ctatgggaac gctcgccgca tttgccacct ttggcgtcgg attccttttc cgtccgctcg
    12721 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
    12781 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
    12841 ttgggtggtg ggcacctatt ttactggtga cgctgcgtgc cattcaggga tttgccgtcg
    12901 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
    12961 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
    13021 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
    13081 ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
    13141 aagagtccgc ggaatttgaa caacagcaat ataatcaagc ggccgcgaaa aaacgcatcc
    13201 cggttatcga agcgctgtta cgacatcccg gtgctttcct gaagattatt gcgctacgac
    13261 tgtgcgagtt gctgacaatg tacatcgtta ccgcctttgc acttaattat tcaactcaga
    13321 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
    13381 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
    13441 tcacaggcgc gttgatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
    13501 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg
    13561 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
    13621 gtgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg
    13681 ccgctgcgct catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
    13741 ctggatgtct gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat
    13801 agtctggcga agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata
    13861 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
    13921 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
    13981 attacgcaac gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg
    14041 caaacaaggt ctttttgtct atccatctct gaccgtaacc tgggacggta gcacaacaaa
    14101 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
    14161 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
    14221 ttatggcgcg catatctcgg tgcaaccctc acagcatgaa atcccttatc cttatgtcat
    14281 cgatggctct gaattaacac ttgatcgctc aatgagcgct gggttaactc gctatttccc
    14341 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga
    14401 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg
    14461 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
    14521 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
    14581 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
    14641 agaagccatt tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac
    14701 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
    14761 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
    14821 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
    14881 tgaccacgtt cttgatgcgg ttctgccacc cgatattcct attccgagca ttgctgaagt
    14941 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa
    15001 acagcggcta cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta
    15061 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
    15121 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
    15181 agataaaccg ttgcatggcg agattaaact tcccggtcag gctaaccgtt tttatgaagg
    15241 cgctatttcc gaacacctac aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
    15301 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
    15361 tctgaacctt cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc
    15421 cgcaataaac gcgggctgtt tgcttccttg ggcggata
//
LOCUS       DAZFKY010000109        15368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFKY010000109 DAZFKY010000000
VERSION     DAZFKY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..329
                     /locus_tag="ACHKCL_003054"
     CDS             81..329
                     /locus_tag="ACHKCL_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5763972.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKCL_003055"
     CDS             307..1437
                     /locus_tag="ACHKCL_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5763973.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKCL_003056"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKCL_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5763974.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKCL_003057"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKCL_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKCL_003058"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKCL_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5763975.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKCL_003059"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKCL_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5763976.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKCL_003060"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKCL_003060"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5763977.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKCL_003061"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKCL_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5763978.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKCL_003062"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKCL_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5763979.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5873..7126
                     /gene="kch"
                     /locus_tag="ACHKCL_003063"
     CDS             5873..7126
                     /gene="kch"
                     /locus_tag="ACHKCL_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5763980.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHKCL_003064"
     CDS             complement(7181..7354)
                     /gene="yciY"
                     /locus_tag="ACHKCL_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5763981.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7497..8957
                     /gene="cls"
                     /locus_tag="ACHKCL_003065"
     CDS             7497..8957
                     /gene="cls"
                     /locus_tag="ACHKCL_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5763982.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8992..9321
                     /gene="yciU"
                     /locus_tag="ACHKCL_003066"
     CDS             8992..9321
                     /gene="yciU"
                     /locus_tag="ACHKCL_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5763983.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHKCL_003067"
     CDS             complement(9374..10378)
                     /gene="oppF"
                     /locus_tag="ACHKCL_003067"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5763984.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHKCL_003068"
     CDS             complement(10375..11388)
                     /gene="oppD"
                     /locus_tag="ACHKCL_003068"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5763985.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHKCL_003069"
     CDS             complement(11400..12308)
                     /gene="oppC"
                     /locus_tag="ACHKCL_003069"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5763986.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHKCL_003070"
     CDS             complement(12323..13243)
                     /gene="oppB"
                     /locus_tag="ACHKCL_003070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5763987.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHKCL_003071"
     CDS             complement(13329..14960)
                     /gene="oppA"
                     /locus_tag="ACHKCL_003071"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5763988.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3910 a         3758 c         3642 g         4058 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata cctgccccaa cataaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat
     2281 caatttttaa atattttcgc tacgcaaata ttaactcgtt cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgccaaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccatattact ggccctggca ccggtgatgc tggtacagga caaaatgggc gtttttgcct
     3001 cgatccgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caaaatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctgaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtcctttgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg caaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagac gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ctgtacttga tgtcgggcgt gccggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt ttttgctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 cggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gagtagagat
     5581 ccaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttataggtc
     5641 ggtcattgca ttcaccacca caagcagata ataactttat ctgtatttct ttatatgatg
     5701 tgagcatgtt caggaaaaat gccctttcct gacgcagagt tgctcactca aaacctttga
     5761 ttactgcgga taaagtcatc tactattgtt taacacattc acgtgtcgca tgtatgttca
     5821 gaaaatgtgc acgttaagat aattaaacaa aattgtggtt acagaggtat cagtgagtca
     5881 ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc acgacattct
     5941 cgcgctggcc gtctttttaa atgggttgct tatttttaaa acaatctatg gtatgtcggt
     6001 caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct ccttgctggc
     6061 aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg gcttactgtt
     6121 ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc taatttacac
     6181 cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta cgctggtgtt
     6241 tttactgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga caatttttgc
     6301 atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt atttaagcga
     6361 aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga tagaaaccat
     6421 gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat tgttcactat
     6481 ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa tttttggtcc
     6541 gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa tgcatcgtaa
     6601 agatcatttt attgtttgcg gacattcgat tctcgccatt aatacgatcc tgcaactgaa
     6661 tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata tcaagcaact
     6721 tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg acagttctgt
     6781 attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg ataacgatgc
     6841 agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca aaacagttct
     6901 cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg atatcattct
     6961 ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa
     7021 taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat ggtattttca gcgataacga
     7081 tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa aaatagagtt taaagacgga
     7141 agagtaaact tattgggcac cgcaatggtg cccagtacat ttagatattg tagatcgcaa
     7201 acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg atacgcatat
     7261 ttcggcgcga ctgcccctca agccaacgcg atttacgtcg gctagcctga cgctgcatgc
     7321 gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc aataacagaa
     7381 tggtcattat acccccgccc ccgacgctga ccagtgcttt tcccgcgttt ttatttgcca
     7441 gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc taatctatga
     7501 caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg ctcattgcag
     7561 gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg gcctggctgt
     7621 tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt ggcgagctcc
     7681 atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc gcaaaatggc
     7741 ttaacgacct taaagcctgt aagcatatct ttgccgaaga aaatagcagt gtcgctgcgc
     7801 cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg aatcagctac
     7861 aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc cagctcgcgc
     7921 gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg gaccaggtgg
     7981 ctgaatcatt aatggcggct gcgcgacgcg gcattcattg ccgattgatg ctcgactccg
     8041 ccgggagtgt agcttttttc cacagcccgt ggcccgagct aatgcgtaat gccggtattg
     8101 aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt atggacctgc
     8161 gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc agcatgaata
     8221 tggtcgaccc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt gatctgatgg
     8281 cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc gactgggaga
     8341 ttgaaacagg gaaacgtatt ctaccgccac cacctgatgt caatattatg ccgtttgagc
     8401 aggccagcgg tcataccatt cacacaattg cttctggccc cggctttccg gaagatctca
     8461 ttcaccaggc attattgact gcggcttatt cggcgcgtga atatttgatc atgaccacgc
     8521 cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg cagcgcgggg
     8581 tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc tgggccagtc
     8641 gcgcattctt tacggaactg cttgctgctg gggttaaagt ttatcagttt gaaggcgggt
     8701 tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc accgttaacc
     8761 ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac gataaaggtt
     8821 ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt ctgctcgatg
     8881 cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg ttttacttct
     8941 tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac tatggatatg
     9001 gatctaaaca atcgcctgac tgaagatgaa acgcttgagc aggcctacga tatttttctc
     9061 gaactggctg ccgacaatct cgatccagct gatgttctgc tgttcaatct tcagtttgaa
     9121 gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca tgttgatttc
     9181 gatttgaacc cagacttttt tgccgaagtt gtgattggtc tggcggacag tgaagatggt
     9241 gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg aaaaagatca caaactttgc
     9301 catatcatct ggcgggaata aataatcact ttcggcgtga aaaagggctg acagctgtca
     9361 gccctttttg tgtttaaagc ggatcgactt tcaggcaaga aacggcgtgt ctgaaactcc
     9421 cctccagcac aggacgtgtt ttggcacact ccggaccggc aatcgggcaa cgggtacgga
     9481 aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt aactggatgg
     9541 ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat gccctggtgt
     9601 aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctacc gcatggccga
     9661 gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca tgagcgataa
     9721 aaattaatga caatcccatc tcacgttgca attgctggag caggttgacc acctgtgcct
     9781 gaattgacac gtccagcgcc gacaccggct catcacagat aatcagcttc ggttcaagaa
     9841 taagagcacg cgcaatcccg atacgctggc actgcccacc agagaactca tgcggatagc
     9901 ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg cgctcgcgaa
     9961 cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg atctcgccga
    10021 tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga atatcactgc
    10081 gaacggcacg ccattcatcg ggtttcatgc ccagcaactc tttacctaac caggcaacat
    10141 gaccgtcggt cgctttaacc aggccaatga tggcgcgagc aaaggtggac ttaccgcatc
    10201 ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagagtt acaccatcta
    10261 cggctttgag cgttttcggc ggttgccaga accactgttt gccatctttg atttcaaagt
    10321 gcactttcag atcggcgatt tcgaggagga cttttcttcc ttcggttaca gcattcataa
    10381 cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact cttccagcgg
    10441 cggtgcgcta ctacaaattt ccatcgcatg cggacaacgt ggctggaatg ggcaaccttt
    10501 cggtaatcgc agcaggtttg gcggattacc cggaatagtc aacattgttt caccttccgc
    10561 atcgagacga ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg gttgataaaa
    10621 gacatcgcgc gcgttgccat attccatcgt gcgcccggcg tacattacca gcactttgtc
    10681 gcagatcccc gccaccacgc caagatcgtg ggtgatcata atgatggcgg tattaaattc
    10741 ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt acggtgacgt ccagcgcagt
    10801 agtgggttca tccgcaatca gcagcttagg tcgacatagc aatgccatcg caatcataac
    10861 tcgctgacgc atgccgccag aaaattcgtg cgggtacatt ttcatgcgtt tacgtgcttc
    10921 cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag ctttgctcat
    10981 gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat agggattcaa
    11041 cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt tattgagttc
    11101 acgctctggc aaattgagga tttcacgccc attgaaggtt gccgatccgc caatgcgtcc
    11161 gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg aaccagactc
    11221 acctacaatg cccagcgttt ctccggcacg tagggaaaaa tttaaatcat tgaccgccgt
    11281 gacgtcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca gcagtgcgtc
    11341 agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg ctgcactcct
    11401 taacgatctt tcgggtcgag ggcatcacgc aagccatcgc cgataaagtt gaaacaaaac
    11461 agcgtcacca cgaggaatcc cgctgggaac aacagtaacc atggagagac ttccattgag
    11521 ttcgcgccat cactcagcaa tgccccccag ctgcttaacg gctcttgcgt acccaacccc
    11581 aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagtagcga tgcgtagacc
    11641 accaccacac cgagtacgtt cggcacaatg tggcgaataa caatgcccga cgtcgataca
    11701 ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt ttgcccacgc
    11761 acaatacgag ccatatccag ccaggaaacc atgcctatcg ccacgaaaat cagcaggatg
    11821 ttttgaccga aaaaggtcac cagcaaaatg acgaagaaca tgaatgggaa ggagttgagg
    11881 atttccagca aacgcatcat taccgaatcc actttaccgc ccagataacc ggaaagcgaa
    11941 ccataaagtg tccccacgac cactgccacc agtgccgcag caacaccgac catgagtgag
    12001 atacgtccgc caatcgcaac gcgcacaagc aagtcgcgac cggatgagtc agtaccaaag
    12061 tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt atcgtcatag
    12121 gcaaactgcg aaagcatcgg tgccaggatt acaaataacg cgatcagcac cagcactatc
    12181 agacttgcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg ccacaagctg
    12241 cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt tttcttactt
    12301 aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc gcatatagca
    12361 catcgacaat ggcattaaac aaaatggtta acgcaccaac caggatggtc aggcttaaca
    12421 ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgcccaatc cccggcaaac
    12481 cataaatggt ttcgataacc atagaaccgg taataatgcc gacaaatgcg gggcccatat
    12541 aggagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc cgccgcatag
    12601 gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca atcatagagc
    12661 cacgggtaat acgcgcaata ctggcgatat aggcgagtga caacgccacc atcggcaata
    12721 tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc aaaatgatcg
    12781 caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc ccggtcattg
    12841 ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcggca ataacgccag
    12901 cactaacacc cagtattacc gcaaggaaaa atgctgcggc tcccagtttg gcagaaacgg
    12961 gaaaactgga tgcaaccagg tcattgaccg aataatcttt atatttaaac gatggaccga
    13021 aatcgccatg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc ggatcattaa
    13081 gatgatattt cgcttcgata ttggccatta cttccggcgg taaagtacgt tcgccggtaa
    13141 aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga ataaatagcg
    13201 tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta ccttctggcc
    13261 tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc ctgccccacg
    13321 tattgccatc agtgcttcac aatgtacata ttccgggtgt aggtattatc cagcggatct
    13381 ttgccggtat aaccaccaac ccacggtttc accagacgcg cattcacgta gtaataaaca
    13441 ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag agctgtgcgc
    13501 tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc cgggctctta
    13561 taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga agttggttcg
    13621 ttgtagtcag cacaccagcc tgcacgggcc acatcaaaag taccctggtg acgggtgtcg
    13681 aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt tttcttccac
    13741 aatgaagagg cagcaatcgc cagcttttta tgcagatcgg aggtgttata caacaggttg
    13801 atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt cgcttcttcg
    13861 ttacgttttt cctggctcca gccaaaccat tccggctgag tcaattttgc gccatcagta
    13921 tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt attaacaatg
    13981 atatcgcggt ccatacccag tttcagtgcg gtacgcacac gcacatcgtt gaatggcggt
    14041 ttctggttgt taatttcgta ataataagtg cacaggtatg gatcaacgtg aacttcgtcc
    14101 gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata agtcatgtcg
    14161 atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatggg caaataggtt
    14221 acctggttaa taacggtttt cgcgttgttc cagtaggttg ggctgcgttc aagaacgatt
    14281 cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat attaccaggc
    14341 tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga agttgatggg
    14401 tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc taatgtgtga
    14461 tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag aatttcatca
    14521 ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcggt gttcggatca
    14581 acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgcc atcagaccat
    14641 ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc ccaggattca
    14701 gcgacgccag gtgccggatg gccgtcaaga tcgctgacca gtaagccttc aaacaggtct
    14761 cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga ctgaacttct
    14821 gaaccattgt tacgtaccag cgtttgtttt tccgccagtg tgacgcctgc gggtacatca
    14881 gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc tactaaactt
    14941 ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact ggtattaacc
    15001 agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg ctgtcaggtt
    15061 actttatcgt tacggattgc tatcaccgac tttatttatt ctggtgattc agacgatcac
    15121 cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc gcgacgctct
    15181 ttaattgtta attattctca ttaccaataa agaatcgcct gacggttgcc cggaaagtac
    15241 caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcaatt ctcttttatt
    15301 gaccgtatca gaatctatag cttgcatcca tgtcttaaca aacatcttta aattcaatca
    15361 aatagaga
//
LOCUS       DAZFKY010000110        15355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFKY010000110 DAZFKY010000000
VERSION     DAZFKY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(240..833)
                     /gene="tehB"
                     /locus_tag="ACHKCL_003072"
     CDS             complement(240..833)
                     /gene="tehB"
                     /locus_tag="ACHKCL_003072"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5763989.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(830..1822)
                     /gene="tehA"
                     /locus_tag="ACHKCL_003073"
     CDS             complement(830..1822)
                     /gene="tehA"
                     /locus_tag="ACHKCL_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5763990.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1946..2926
                     /gene="ydcK"
                     /locus_tag="ACHKCL_003074"
     CDS             1946..2926
                     /gene="ydcK"
                     /locus_tag="ACHKCL_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5763991.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2921..3457)
                     /gene="rimL"
                     /locus_tag="ACHKCL_003075"
                     /pseudo
     CDS             complement(2921..3457)
                     /gene="rimL"
                     /locus_tag="ACHKCL_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3520..3744)
                     /gene="ydcH"
                     /locus_tag="ACHKCL_003076"
     CDS             complement(3520..3744)
                     /gene="ydcH"
                     /locus_tag="ACHKCL_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5763992.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3884..5539)
                     /gene="opgD"
                     /locus_tag="ACHKCL_003077"
                     /gene_synonym="mdoD"
     CDS             complement(3884..5539)
                     /gene="opgD"
                     /locus_tag="ACHKCL_003077"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5763993.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5764..7107)
                     /gene="ydcJ"
                     /locus_tag="ACHKCL_003078"
     CDS             complement(5764..7107)
                     /gene="ydcJ"
                     /locus_tag="ACHKCL_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5763994.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7324..8247
                     /gene="ydcI"
                     /locus_tag="ACHKCL_003079"
     CDS             7324..8247
                     /gene="ydcI"
                     /locus_tag="ACHKCL_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5763995.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8285..9925)
                     /gene="trg"
                     /locus_tag="ACHKCL_003080"
     CDS             complement(8285..9925)
                     /gene="trg"
                     /locus_tag="ACHKCL_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5763996.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            10324..10473
                     /gene="hokB"
                     /locus_tag="ACHKCL_003081"
     CDS             10324..10473
                     /gene="hokB"
                     /locus_tag="ACHKCL_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5763997.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(10451..10519)
                     /gene="yncP"
                     /locus_tag="ACHKCL_003082"
     CDS             complement(10451..10519)
                     /gene="yncP"
                     /locus_tag="ACHKCL_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5763998.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10545..10718)
                     /locus_tag="ACHKCL_003083"
     CDS             complement(10545..10718)
                     /locus_tag="ACHKCL_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5763999.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(10963..11493)
                     /gene="cybB"
                     /locus_tag="ACHKCL_003084"
     CDS             complement(10963..11493)
                     /gene="cybB"
                     /locus_tag="ACHKCL_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5764000.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11682..12683
                     /gene="gap"
                     /locus_tag="ACHKCL_003085"
     CDS             11682..12683
                     /gene="gap"
                     /locus_tag="ACHKCL_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5764001.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12725..14164)
                     /gene="aldA"
                     /locus_tag="ACHKCL_003086"
     CDS             complement(12725..14164)
                     /gene="aldA"
                     /locus_tag="ACHKCL_003086"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5764002.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14361..15161)
                     /gene="ydcF"
                     /locus_tag="ACHKCL_003087"
     CDS             complement(14361..15161)
                     /gene="ydcF"
                     /locus_tag="ACHKCL_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5764003.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15277..>15355)
                     /locus_tag="ACHKCL_003088"
     CDS             complement(15277..>15355)
                     /locus_tag="ACHKCL_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764004.1"
                     /translation="EHYVIATSNDVIDVLSTVSPQIVYD"
BASE COUNT         3983 a         3881 c         3583 g         3908 t
ORIGIN      
        1 tggcaaaaaa agtgttggtg aaaataaatt tttttagccc cttttcagac gacatagtca
       61 atgccgcaaa aggggctaag tcaatcatca gtagttaaat ttcagtaagt taagctgcta
      121 aaaaacttcg actactgtga ttaatttcac atcccttcga taaaaaatat gcttaccgaa
      181 aagaaggaca gtaaaccatc tcagcgtggg gaattttcac gcgctttgtt cttaccgggt
      241 cattttttac gtgccagcat cgtggcgaaa cgcagtttaa tacgattacc gttggcgtcg
      301 gtgcggtgca gctcgccgac gtcttcattg tatttcacca tctcccagcc ttcgtaatat
      361 cgacgtaatt ctccctcttt gaaggcaaac gggaagccga cggtacatgg ataatcagcg
      421 gtatccatcg ccgccacaat caggttgtaa ccgccaggtt tagtgcaacg ttgcatattg
      481 gcaatcagcc ctgggatggt tttagcctca aggaacatca gcaccacagt cgaaagaata
      541 aaatcgtact ctccatcaaa tgtgaggtta ttcagatcaa cgacccgggt gtgtaaatta
      601 tccagatttt caatggattt aatgcgctcg acgttggcga tactcatggc atttttatcc
      661 catgcgtcaa catcataacc attggctgcc aggtaaagac tgttacgacc attgccacag
      721 cccagatcca gcgttttacc cggtttaacc actttcaccg cttccagtac ttcagagtgt
      781 gtgcgggtta attcatattt atcagtaaaa taattttcgt cacgaatgat cattctttgt
      841 cctctgcttt cattaaaacg gcgcgctcgg ttctgactaa caattttccc tgcatcagaa
      901 gcgccaaagt acggatgagc agtattgcaa taataaaatt ggtaaagata aacagcggca
      961 ccgccagcgt atggaaaaat ccattatcgc tgccactccc cagatgcaaa ccggtggttg
     1021 ccagtgcaga tacgccgaac gagaaactcc agaatgaagc attaaatggc tgggagagat
     1081 accatggcat cagacgtagc ataaacagca gttgcagcag tccataaccg aaaagcattt
     1141 tcgccagcgt gtcaccctcg ccgccgttga cgctcagcca ggcactacag gccaccagcg
     1201 caggagcgag ctgaatgccg agtgatgtcc gcagtgccgt gggtaattct cccgaactac
     1261 gcagacgctg caagatcacc ggttcaaggc ttagccatga gaaaacgcct gcgcctaaaa
     1321 acaccagacc ggcgtcggtg tagcccaacg caccacaggc cattgcgctg ataaagttgt
     1381 tggcaactgt cggcagatac agtccaggcg tagtagcttc ttcagggtga gatccgcgcc
     1441 ataatcccgc agtttgccag gcggcataag ccaactgaac cacgacacca aaactgaaca
     1501 ggcacaccgc cagtgggcga aaccacggaa caaaaccaat cgccaccagc atcgtcgttg
     1561 caggaaacaa actcacaaaa ctgctcagca ctggatggcg aacttccgcc agcacgctat
     1621 gcggaaagcg tatgagtcgg gtaataaatg cgctagtcaa taatccccag atgatcatcg
     1681 ccagaatcac cagcccatcc cctaaccagt ggctgacctg ccaaacctgg ctggcatagc
     1741 gccaggcaaa tcccatccct atcgtcccca acacaatacc aaagtagcct gccggcaaat
     1801 tgagcacttt atcgctctgc atcttcttac tcattttgtt taatttataa agtatatttg
     1861 aaatgcattt tatggaattt tctcgagcat agccagagcc gcagaatttg ctacggttat
     1921 agtaagtcac ctgtgggaaa acgctatgcg caaatatcgt ctaagtgaag aacagcgagc
     1981 ctttagttat caggaagatg gcactaaaaa aaatgtgtta ttacggcaga ttatcgccat
     2041 tagcgatttt aacgatgtga tagcaggaac cgctggcggc tggatcgatc gcgaaacggt
     2101 gttagcacag gaaggaaatt gctggattta cgaccagaat gctattgcgt tcggcggaac
     2161 ggtgatttcc ggtaatacgc gcatcaccgg caccagcgta ctgtggggag aggtttacgc
     2221 aacggataat gtctggatcg ataatagtga aatcaaccag ggtgcatata taagtgacag
     2281 tgtcaccatt catgactcac tggtttgcgg gcaatgtaga atatttggtc acgccttaat
     2341 tgatcaacat tctatgatcg tcgccgcaca aggcttaacg cccgaccatc aactcctgct
     2401 ccaaatttat gatcgcgcaa gggttagcgc ctcgcgtatt gtccaccagg cacaaattta
     2461 tggcgatgcc gtcatccggt atgcttttat tgaacatcgc gccgaagttt ttgattttgc
     2521 cagtattgaa ggtaatgaag agaacaacgt ctggctatgc gattgcgcta aagtctacgg
     2581 tcacgcgcag gtgaaagctg gcatagaaga agatgccatt ccaacgattc attacagttc
     2641 gcaggtggcg gaatatgcca ttgtggaagg taattgtgtg ttaaaacatc atgtcttgat
     2701 tggcggaaat gccgtggtac gtggcggacc gattctgctc gacgagcacg ttgtaataca
     2761 aggtgagagc cgtattacag gtgcggtgat aattgaaaac catgtggagt taaccgacca
     2821 cgccgtcgtt gaagcctttg atggcgacac ggttcatgtc cgtggtccaa aagtcattaa
     2881 cggcgaggag cgcattacgc gaacgccgct ggcagggtta ttatgaatcg ataatacgcg
     2941 cgtataagtt cacatcatca taggcatcat tcaggaactc agcctgtttc aggcaacctt
     3001 caaggataaa accattgcgc aaagcgacct ggttgctttg cggattgtcc acccgacatt
     3061 tgatcacgaa gcgtctaagt tcaccagact gagcgtaatg atgaatcagt gcctgcagcg
     3121 cctgagaaat gatcccctgc ccctgatgag attcgtccag tcagtagccg atttcagcgg
     3181 ttttattcag tggttcaata cgattaaacg agataacgcc gataagttca tcttctttga
     3241 aaatcatgaa cattttggca tagccgcgtt gatgcaacat cacattaccc tgcaccgttt
     3301 ttcgcgtgtc ctcttcaatc tgaacgaact gcggccagtt tagcgactgc tgtaaccagg
     3361 ttttattttt acagattaac tgataaagag gagtgacgtg attttctgca acagcatgta
     3421 attcaagtga ttcgcttact tttatcgttt cagtcatctt atctccagtc aatccatgca
     3481 gttgttaaaa aagccgcctg gctggcggct taggaaagtt tacacctctt tgacgctctc
     3541 atgctgcagg attttgagca tctcatcttt taactgtagc ttttgttttt tcatgcggac
     3601 cacttccgca ttgtaccctc gatcgtcgga accttccttt ctggcaattt catgatcaag
     3661 tttattgtgt ttatcgaaca aggacataaa gcgaggattt tcgtttttca gacgggatat
     3721 taaatctcga tattctggaa acatacgcac tccctgttag tggctgacaa ggtgtatctg
     3781 aaatacacac catcagatta ctcatttaac ttagggaaaa atgcgagcgg gatcgtgttt
     3841 aaccagacgg cacctcccga agaaggtgcc gaagaaaaaa cgattaactc atcacgcggt
     3901 cgtcaacata ctgacgttta tccggcgctg gcgggaaata ctgatacagc catgtttcac
     3961 tgatagcgtc gccctgacaa cgcagataca tccgcatatc gaccggatca gtggagtccg
     4021 aagtcggata ccagtcaaac tgaatacgat aaccatcaat gggttcaata tagagaattt
     4081 cgatttgctt cgcttcccca ctggaaagcg taatcaccgg ctcaatgcct tttggtgcgg
     4141 cagctttcag atcaccacca acgaaatcga cggcaaaacg acgcgcccat ttttcgggat
     4201 agtgttcacc tggtgcccaa ccttccggga aaccgcccat gccggtacgc gtcgccataa
     4261 cgcgcgctaa tgggcaatga acaggcggtt gcgcactcca gtacagacga tactggaatg
     4321 caaactcatc acccgccttt acagcttttt ctggctgcca gaagcagaca atgttatcca
     4381 gcgtttcgcc cgttgttggg atttccatca ggccgatggt gcccttaccc cacttgttac
     4441 gcggttccac ccacagactt gggcgtttgt tataccagcc cataatgtcc tgataatggg
     4501 agaaatcacg atccagttgc aataaaccaa accctttcgg gttgttgtcg gtgtaagcat
     4561 tgaactgcaa tttttgcgga ttattcagcg gacggcaaat ccactcgccg ttgccccgcc
     4621 acatggacag acgatcagag tcatgaattt gcggatgaat ggtgtcgcac atccgacgtt
     4681 cattagtacc gcagctgaac atactggtca tcggcgcaat gcccagctgt ttaatgtctt
     4741 tgcgcgcata caggtgattt tccacatcca taatcacctg acttttctca caatggatag
     4801 tgaacttata ggcaccagta atgctggcgc tatcgagcaa cgcataaacg gtaaaggtag
     4861 ttgcccccgg ttttaccgta tcaaaccaga aggcggtaaa gtcggggaac tcttctttac
     4921 tgtcggtgta agtgtcaatc gccaggccgc gggccgacaa accgtattga tatgtgtcat
     4981 caacggcgcg gaaataactc gctccgagga atgatactac atcacggcgc gccagttcgg
     5041 gggctttaaa cacgcgaaaa ccggcaaagc cgagatcgct ttgcccttct aattgtttgg
     5101 tatcaacacc tgcatcgttg tatttgaaca actccgggcg aaagtgaatt tcacgcgcca
     5161 gatgtgttgc tggatctaca gaaaacatac gaacgcggcg acggaatccc attcccatat
     5221 ggaagaactg agcgtccagt tgacggttct caacgttatg ccagagcgat ttttcggcgt
     5281 cgtattgaat actgttataa gcctgcggcg tcattgtcgc cagcgtgtca ggtaacggac
     5341 gaggcgcacc acgccacgct gtttgcgcta agtcgtgcgc cattgactgt agaatggaga
     5401 agtcaaaacg ctgggtttgc ccgtcggcaa tatcagaatc tgccgcgaat gccgcctgag
     5461 aaaaaagaga agcaatgccg ctggtaccgc acacggcggc catagccatt gaacctttaa
     5521 taaatcgtct acgatccata cctgaaagtg agtccttctg gtgttgtgaa tggtcccgcg
     5581 ctggtcgttt tcagcggcaa gaaagtgctt atttatgacc gctcactcta gacaatattt
     5641 attaataatc caattgttga actgagagaa tatgcataaa tcggcaattt ttttatgtcg
     5701 atgggatgag cctgattcgt ttgtaaaatc aggcaaattt ttgcaaaggc ggatggtcag
     5761 aatttaaagc aaaccacagc gacgtttact gcgttcttcc gcttcctgat aaagctggaa
     5821 ctcatccaaa accggacaac ccaacgcctg ctcaaatgct tcgcgactgg cattaccgtg
     5881 gctgcgtgcc tgcgtttcat tacccagatt tgactggaag atccccgccg cgctaacggg
     5941 caagaaatct tcataggtaa tgggttgcgc cgcgacccaa ccacgttcaa ttaagggctg
     6001 tggatcgtct ccgggatgaa tcgcctgacg atgcgcctca cctgaaggcg tcagacggta
     6061 ccggaaccat gccagccctt gctgacgcat taaaaactca ctgtcaggaa aagtgcggaa
     6121 ggtttcctgt aaatgcattt ggtgagtgag attatcctgc ccggttccag cgttacgcag
     6181 aagatcatca tacagttgtc gacctttcgg cgttaatgcc acgccacgct gctcaatttc
     6241 accaaagcgc gcagtatgcg tgccctgttt ctgccccgca aacaacaccg tctcttccag
     6301 tgctttaaag ctggtctggc gtagtaaaat cggtacctcg cggcgcggcg gcccttcgat
     6361 cagaattttt ggttcaattc cgcattcagg catcatcgac tgcacccggt caatatccag
     6421 cgtacgtggc gtcagatggt tgatatggca tccaggaaaa cagaccacat cagcaatcaa
     6481 ccgatgttcg ttgtgcaagg cgcgataggt ttcttcatct accgttgctg actggtgcca
     6541 acggaacgtt tccagggcct cctgcacaaa ctcctgtgcc tgtgtttcgt taaaaccgcc
     6601 ccgctggtca tactcctcta acagttgtcg acaacgtggg gtgaagatat cgcgctgacg
     6661 cagaatctcc gccgctttct ggcgcaaaat ttcgttctcg ataagctcaa ggcggagtaa
     6721 ggaggtaaaa acgcggaagg gattacgcgc cagagaagca tcatcaatgg gccgaaatgc
     6781 tgtcgaatgt accggcaccc ctgcctgcga gagatcgtaa tagctcaccg ggtacatccc
     6841 cataatggca aacatccgcc gaagagtagc aagctcttgt gcagtcccaa cgcgaatcgc
     6901 cccatgacgt tcaacattca gtcgcgccag ctcgtctgca tttaccattt tttcgtgcag
     6961 ttgaggattg ttttccagca cagccagatt cacatcagct accagttcca gcagcgtgcc
     7021 atattgcgga acttcttgct ggtacatggc tgacattgcc tgcgaaaact gttcccgaat
     7081 ctcatccgcc gtgatgctgt tcgccatagt gtctgcctcc agtggtcagt aatctggagt
     7141 gtaggtaacc gcattcactc tggcgggaag aatttacaaa ctgtgatctc gccgcgaaaa
     7201 catcaatatt atccattttg ctgtaacata attgctttaa ttgttaataa tattttgcaa
     7261 tcaagttatc ataatcaaac aacttcactt gtcagcgaca ccgcttcgtt tttaacatcg
     7321 cttatggaaa aaaatagtct gtttagtcag cgcatccgtt tgcgccacct tcatacattc
     7381 gtagctgtcg cacaacaagg aactttgggg cgcgcggctg aaacccttaa tttgagtcaa
     7441 cctgcgctct ctaagacatt aaatgaactg gagcagctga cgggcgctcg cttgtttgag
     7501 cgtggtcgtc agggggcgca acttacctta cccggcgaac aatttttaac gcatgcagtc
     7561 agagtacttg acgccatcaa cactgccgga cagtcgcttc atcgtaaaga aggtcctaat
     7621 aatgatgtcg tcagggttgg tgcactacct actgcggcac tggggatatt accttcggtt
     7681 ataggtcagt ttcatcagca acaaaaagag acgaccttgc aagttgcgac aatgagtaac
     7741 cctatgattc tggcgggttt gaaaaccggg gaaatcgata tcggcattgg tcggatgtca
     7801 gatcctgaac tgatgaccgg gcttaattac gaactgctgt ttcttgaatc gctgaagctg
     7861 gttgtccgcc ctaatcatcc gctacttcag gagaacgtaa cgctaagccg ggtgctggaa
     7921 tggccggtcg ttgtatcacc agaaggcact gcgccacgcc agcattcaga tgcattagta
     7981 cagagccagg gatgtaaaat tccttcgggt tgtatcgaaa cgctgtctgc ttcgctatct
     8041 cgtcaactta cggttgaata cgactacgtg tggtttgtcc cttctggcgc ggtaaaagac
     8101 gacctgcgtc atgccacgct ggtggccctg cctgttccgg gacatggtgc aggcgaaccg
     8161 attggaatac tgacccgcgt agatgcgacg ttctcttctg gttgccagtt gatgattaac
     8221 gctattcgaa aatcaatgcc gttctgaaag gtgaagggat ctgtcgatcc ctcctttaac
     8281 attttcacac cggagcgaaa ctaactggtt cacccgctcc gcgaggttct gccaacacag
     8341 attgtttgtt cagacggaat acgtccaccg cctcagttaa tcgtgccgcc tgttcttcaa
     8401 gggacaccgc cgccgctgag gcctcttcta ccagagaagc attctgttgc gtcaccttat
     8461 ccatttcaga aatcgcctgg ctaacctgcg ttatgcccct actttgctca tccgaggcgg
     8521 cggcaatttc ctgcatgata tgtgtgacac tcgcgacggc atcaacaata gtgctcatgg
     8581 ttttcccggc cgttgccacc tcatccgatc ccaggtcaat taacctgact gattcactga
     8641 tcaagccttc aatttctttt gccgcctggg cgctgcggct tgcgagtgtc cgtacttcgc
     8701 tggcgacaac ggcaaatcca cgcccttgct cacccgctcg cgcagcttca acggcagcat
     8761 tcagtgccag aatattcgtc tggaaagcaa tactgttgat gacggcggtg atctcagaaa
     8821 ttttcttcga actcgtggag atagcgccca tcgtttttac tacaccggaa accgtctgcc
     8881 cgccatcgct ggctttaata gaagcctctt gcgccagttt gctggcataa tgcgcgttat
     8941 ccgcattctg tttcaccgtc gcagtgagtt gctccatgct ggcggcagtt tgttcgatag
     9001 ccgccgcttg ttcttcggtg cgagatgaca ggtccgcatt gccagctgaa atttcgctgg
     9061 tgccacgata aatctcttcc gcaccctgtc gaacagtccc tacggtcatc cccagtgaat
     9121 gctgcatttg ctgtaaatga cgacttaagc gaccgatttc attacgaccc gccggttcat
     9181 ccttcatcgt cagatcgcca ctagcgattt tttcaatccg ttgtgcggca tgttgcagtg
     9241 gacgaatgac gatccgacgt agcaccataa atgttatcag cgtcatgacc agggcaagca
     9301 caaacgcacc aatcatgaac atcccgccca ggcgggtgcg ctgatgggcc agttccgcca
     9361 gttggttggc tctggtgcta cgtatcttaa cggctttgtt caaaatatcg gtataagcat
     9421 tatccagcgt tcggatctgc tcactttcat gattgataat cgcttcaaac atgccatttt
     9481 tggcatattt caacataggt tgcatacccg tgatataagc ctgaaagcgt tgatttaatt
     9541 cagtgtcgag ggcttcatca gcaggtgttt tcaccggccg attctgataa gcacgataac
     9601 cttgctgcga ctgtttaatc tccgattcgg cttgcgcaat atttcgcttc attgcttcca
     9661 tttccgcaat acgactcgca gccccggcct gaatcatatt gatccgggct gaacgcaaaa
     9721 aatctgaact gttcgctaac ccggtccgaa tctcaatctc cgcagtaaca tcacgctgat
     9781 cgcgatcggc ctgccagagg aaatagccag ccagggctga acttaatgcg aatagaacta
     9841 agataccgcc tagaatgcag gcaaataacg gaaccaacct gatgtgatgc aaaaaaccta
     9901 atcgctgtga gggagttgta ttcattaccg ttgtctctcg tccaggttta ctcctgatag
     9961 aaagtcatcg gcatttgacg gtaattactt acgactaaaa gcctctttgc gtcacatttt
    10021 tacaacaatt attgcagaga agattaatgc cattggaatg aagagagtaa cgaatggtta
    10081 aactggctaa cccgaatgac gaaaaattct ggaaaacagc acgcaatttc gtcaggtgat
    10141 tattttcgtc cctaaggaaa ccacatagga tgctcacggg tgcctgagac tttctgtttt
    10201 caggctaacc gggaatcaga aagacgaagg ccccgaccga tatttctatc aaccgaggcc
    10261 aacgaatgaa ccaagcaatt cagatgttag cctcttaccc gccatccggc aaggagaaag
    10321 gctatgaagc gcaaccctct ggtggtgtgt ctgctcatta tctgcattac gattctgaca
    10381 ttcacactcc tgacccgaca aacgctctac gaactgcggt tccgggacag tgataaggag
    10441 gttgctgcgc tcatggcctg cacgtccagg taagggcaag cgcggggatt ttccccgcgc
    10501 atctgactag cagattcatt ttctcatggc acccctttta cacttcagtt agtgcatgtt
    10561 tttttcgatg cactaataga accatcatta cagacaaatt tgccgcctgc cgtacagtgt
    10621 gagatccccc ctttcgaacc agaacagggt ttgcgcccgg catcggcata aaaggaaaca
    10681 agcgctccca taaacaaaat aagtgccagt tttttcatat aacctccatg gtttatcgaa
    10741 tcttatgttc gatttgaacg ttatacgctg gtttattttg ataccgttga tgagatcaaa
    10801 gatcgggtga attgattaaa aataagccag atggacgtat gggcaaggat tatggtttta
    10861 tttgtcatac aaataaatat aataggcgct tccgatgtag acccgtattc ttcgcctgta
    10921 ccacgggtcg gttttagtac aggcgttttc tttaaatatc cttcaggaac gtttacgcgg
    10981 catcatgcgt agaagtgtgt tgtccttcca gaaatagtgg tgtgccagtg cagccgcagc
    11041 gtgcaaaccg atgacaaaat atcccagatt cgccagcgtt tcatgccacg actttaagct
    11101 atctacccgt tcgaaattgg cctctgaagc gtaaggcatc gtcaaaccaa acgcaaacca
    11161 cgggttgcct cggttataca tcatcaccaa accaatcact ggcagcgcaa taaacaggag
    11221 ataaatcacc aaatgtccca aatgcgccag tcccgtcatc atcggttttg gcttaggtat
    11281 aatcggcggg gttgggtatt tcagccttaa caaaagacga acgaccatca gcacgagaat
    11341 tgatatgccg caggaaacat gaatcatgtt gatgagtggc cgatcgctac gtgggaagaa
    11401 accccgaaac tccattgcgc aatacgctgc gataaccagc aaaaagacca gccagtgaat
    11461 gctgatttgt aaccttgagt atttattttc cataacattt cctgctttaa cataattttc
    11521 cgttaacata acgggctttt ctcaaaattt cattaaatat tgttcaccct ttttcagtac
    11581 gttacgtttc actgtaagaa tggagattgc ccatcccgcc atcctggtct aagcctggaa
    11641 aggatcaatt ttcatccgaa cgttcctgac aggagaaaac catgagtaaa gttggtatta
    11701 acggttttgg tcgtatcggt cgactggtgt tgcgtcgatt acttgaagtc aaaagcaaca
    11761 tagacgttgt cgctattaat gatcttactt ccccaaaaat tctcgcctat ctgctgaaac
    11821 atgattcaaa ctatggtccg ttcccctgga gcgttgattt tacggaagat tcacttatcg
    11881 ttgatgggaa aagtatcgcg gtttacgccg aaaaagaggc taaaaatatt ccgtggaaag
    11941 cgaaaggtgc agaaatcatt gtcgaatgta ctggctttta tacctccgcc gagaaatcgc
    12001 aggcgcatct tgatgctggc gcgaagaagg tgttgatttc cgcccctgca ggtgaaatga
    12061 aaactatcgt ttataaagtc aatgacgaca ctctggatgg caacgacacc attgtttccg
    12121 tggcatcatg cactactaac tgtcttgcgc cgatggccaa agccttgcat gacagtttcg
    12181 ggatagaagt cggcacgatg acgaccattc atgcctatac tggcacccag tcactggtgg
    12241 atggcccgcg tggtaaagat ttacgtgctt cacgcgccgc ggcagaaaat atcattcccc
    12301 acactacggg cgcggcaaaa gccattggtc tggtgatccc ggaactgagc ggcaaactga
    12361 aaggtcatgc gcaacgcgtg ccggtgaaaa caggttcggt cactgaactg gtatcgattc
    12421 tcggaaaaaa agtgactgcc gaagaggtga ataacgcact taaacaagca accaccaata
    12481 acgagtcatt tggttatacc gatgaagaaa tagtctcttc cgatatcatt ggcagccatt
    12541 tcggttcggt gtttgatgcc acgcaaacgg aaattaccgc cgtgggcgat ttacaactgg
    12601 tgaaaacggt cgcctggtac gacaacgaat atggcttcgt cacgcagctt attcgcaccc
    12661 tcgaaaaatt cgctaaactc tgacgcgcac aggcggagga aaaaacctcc gcctctttca
    12721 ctcattaaga ctgtaaataa accacctggg tctgcagata ttcatgcagg ccatgtttac
    12781 catctgcgcc gccaataccg gatttacgcc atccggcgtg gaagccttgc atagcttcga
    12841 agttttcacg gttgatgtaa gtttcaccaa acttcagccc tttaatggct ttcatcgcga
    12901 cgttcagatt ttgggtatag attgatgagg tcaggccgta atcactgtca ttagccattg
    12961 agatagcatc ttccagcgtg tcaaatgcga ctaccggcag aaccgggcca aaggtttcct
    13021 catgcataat cgacatttcc tggcgaacat ccagcagcaa tgtcggcgga taataatatc
    13081 ctttcccctc taccgctttg ccacccaacg ccactctcgc cccttcttct actgcgcgcg
    13141 ccactttttg ctcgaccctt tccagcgccg cggcgttaat caacggcccc atcgcaatgt
    13201 cgttgcgttc agcggggtta ccaaattgaa ccgcctgcat cgcttcaccc agccgattga
    13261 cgaactgatc ataaatgcct ttctgtacat aaacacgttc tgcacagtta cacacttgcc
    13321 cactattaat gacgcgtgaa tcaacgatgg ctttgactgc cagttcaaga tcggcatcgt
    13381 ccattacgat agctggtgct ttacccccca gttccaggca cactttggtg atgtttttcg
    13441 ccgcagtcgc catgatcttc tcacctgcag agacgctgcc tgtcatactg accattgcga
    13501 cctttgggtt acccgccagt tcttgcccaa cggtttcacc acgccccaga acaaggttaa
    13561 acacgccgcg cggaaggcct atttcatcga cgattttggc gaatgcaatc gcattgtttg
    13621 gcgtaaattc actgggttta atgacaatgg tattaccagt caaaagagcg ggagccattt
    13681 tgcgggcaat gaggaagaac gggaagttcc acggcagaat gccggtagtc acaccaagcg
    13741 cacgtttaaa cagaagaata ttttctcctg gacgatcgct ttgaataatc tcgccctcgt
    13801 aacgccgtgc ccactccgcc atgtaatcga tatagtcggc agtaaaagcc acttcgactt
    13861 cagccagctg ctggatcttg cccccttctt caacaatcag cgcactgatt tcactggcgc
    13921 gttcgcggat cccggcggag attttgcgca accaactggc gcgttcaatt gcaggcaacg
    13981 cttcccattc tggttgtgca cgttctgctg catcgattgc cttacgggca tcctcggcct
    14041 gaccatcggg aatgcgggaa atgacagcct ctgtagcagg gtttaccaca tcaatccatg
    14101 cgtctccacg ccaggtaaca aactgtccat caatatacat aggatgttga acgggtactg
    14161 acatgggcga ctcctgtgat ttatatgttt gtttttggca aatacattgt taattaacat
    14221 caatcatcat agagacggca tcatgaaagg acaattctgt gaagggcttc ataaaaacaa
    14281 gtgtaaaagg aataagaggc agaaataaaa ggcaggagtt atgcccgttt gcaaacaagt
    14341 ggttcacaaa cgggcaattt ttaacgtaac gagcgacttt ccatcgcctc gatgagcacc
    14401 gcatcgtgtt tcagcgtttg ccatgcatgg atgacttctg ccggaaaatc aacgtgaacg
    14461 ataaaatctc gcccgcgggg accgtagcca tcgctatcat cgcgtaaacg cggcagctcg
    14521 ccagtgagta gtgagagata acgctcaacc ggccataatc cttgtaactg attaataaag
    14581 attacactgt ctgcgttatt tcctaactga ggaacgaatc cgggataact taaccagcgt
    14641 ggtacatcgg gattgtcccc ggtcatacgg cggaacgtcg ccatcgtgcg ccgctgcatg
    14701 gtggggtcct gtacaacgat agctgtgtga actcgttcta cggcctgatt caatagcgcg
    14761 atgctaaagc gtgcgttttc accgcagttt gttgactggt cttcaatcca gattttttca
    14821 tgcggaatat gccagaactg atgagcgata tccgccagga tggtcgcttc tgctctgcca
    14881 gtggtgcgga tagtgttgta gtgcggatgc tgtgcgatgg cgctatacaa aaaagtggtc
    14941 gagtgaccga taccaccact aatcagtaaa ggaatttgct gatcgcgagc aatcttacat
    15001 gccgcatcga tagtcggcat aaccgcattt cctgcaagta tgacgcaatc agcctgatac
    15061 ggcacctcac cggagaaatc atcctgcgcc agccactgtc cgataacatt tatggcatct
    15121 atagttgccg tcgaaagcgt cggaaacggg gtaatgttca tggttcctcc ttatcgttcc
    15181 cttaagaata ctgcgtgaga taatagtgag aagcggagca ctctgaaagg aagataatgc
    15241 tttttctatc tcgccatcgc gaaccagaca ctggttttag tcatacacta tttgcggaga
    15301 aactgttgat aaaacatcaa taacatcatt tgacgtggca ataacataat gttcg
//
LOCUS       DAZFKY010000111        15193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFKY010000111 DAZFKY010000000
VERSION     DAZFKY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..1082
                     /gene="ybeQ"
                     /locus_tag="ACHKCL_003089"
                     /pseudo
     CDS             105..1082
                     /gene="ybeQ"
                     /locus_tag="ACHKCL_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1152..1634)
                     /gene="ybeL"
                     /locus_tag="ACHKCL_003090"
     CDS             complement(1152..1634)
                     /gene="ybeL"
                     /locus_tag="ACHKCL_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5764005.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1870..4452
                     /gene="leuS"
                     /locus_tag="ACHKCL_003091"
     CDS             1870..4452
                     /gene="leuS"
                     /locus_tag="ACHKCL_003091"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5764006.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4467..5048
                     /gene="lptE"
                     /locus_tag="ACHKCL_003092"
     CDS             4467..5048
                     /gene="lptE"
                     /locus_tag="ACHKCL_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5764007.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5048..6079
                     /gene="holA"
                     /locus_tag="ACHKCL_003093"
     CDS             5048..6079
                     /gene="holA"
                     /locus_tag="ACHKCL_003093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5764008.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6081..6722
                     /gene="nadD"
                     /locus_tag="ACHKCL_003094"
     CDS             6081..6722
                     /gene="nadD"
                     /locus_tag="ACHKCL_003094"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5764009.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6746..7357
                     /gene="cobC"
                     /locus_tag="ACHKCL_003095"
     CDS             6746..7357
                     /gene="cobC"
                     /locus_tag="ACHKCL_003095"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5764010.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7617..7934
                     /gene="rsfS"
                     /locus_tag="ACHKCL_003096"
     CDS             7617..7934
                     /gene="rsfS"
                     /locus_tag="ACHKCL_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5764011.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7938..8405
                     /gene="rlmH"
                     /locus_tag="ACHKCL_003097"
     CDS             7938..8405
                     /gene="rlmH"
                     /locus_tag="ACHKCL_003097"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5764012.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8436..10337
                     /gene="mrdA"
                     /locus_tag="ACHKCL_003098"
     CDS             8436..10337
                     /gene="mrdA"
                     /locus_tag="ACHKCL_003098"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5764013.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10340..11452
                     /gene="mrdB"
                     /locus_tag="ACHKCL_003099"
                     /gene_synonym="rodA"
     CDS             10340..11452
                     /gene="mrdB"
                     /locus_tag="ACHKCL_003099"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5764014.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11463..12551
                     /gene="rlpA"
                     /locus_tag="ACHKCL_003100"
     CDS             11463..12551
                     /gene="rlpA"
                     /locus_tag="ACHKCL_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5764015.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12691..13902
                     /gene="dacA"
                     /locus_tag="ACHKCL_003101"
     CDS             12691..13902
                     /gene="dacA"
                     /locus_tag="ACHKCL_003101"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5764016.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14012..14275
                     /gene="ybeD"
                     /locus_tag="ACHKCL_003102"
     CDS             14012..14275
                     /gene="ybeD"
                     /locus_tag="ACHKCL_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5764017.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14376..15017
                     /gene="lipB"
                     /locus_tag="ACHKCL_003103"
     CDS             14376..15017
                     /gene="lipB"
                     /locus_tag="ACHKCL_003103"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5764018.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3762 a         3842 c         3950 g         3639 t
ORIGIN      
        1 tatacttttc tgacgcgaca taatacgcat aaaataaggc ttgcgcaaaa aatgaaacac
       61 ataaaaattc aatatatttt atctacaatc ataaggaaat gataatgatt ttcacgtcaa
      121 gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg
      181 aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg
      241 acgaaaaggc cttttactag ctgaagctgg ccgctgagca aggtcattgt gaagcacagt
      301 attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca
      361 tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct
      421 ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttgcctgg tatcagatag
      481 ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca
      541 acggagccgc acaagactat acgctggcat ttttctggta caaacaagct gcattacaag
      601 gccatagtga cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc
      661 aaaacgagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg
      721 ctcagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc
      781 aagcgatgta ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca
      841 tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat
      901 ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt
      961 atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag
     1021 ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt
     1081 agagataaaa acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg
     1141 aaatcagact attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg
     1201 catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc
     1261 agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg
     1321 aaaacttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag
     1381 ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag
     1441 ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt
     1501 cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata
     1561 tcacgttcgc cattgcgcag gcgttcgctc agtgacgcaa ccagttcacg gtaatattga
     1621 gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct
     1681 ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg
     1741 gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct
     1801 atgcggatct gaaaaaccac ctctaacgct acttttgtag ccgtaatgaa aacaggacca
     1861 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
     1921 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
     1981 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
     2041 atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc
     2101 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg
     2161 gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt
     2221 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
     2281 aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac
     2341 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
     2401 cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct
     2461 tacgctgacg agctgctcaa cgatttggat aaactggatc actggccaga caccgttaaa
     2521 accatgcagc gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt caacgttaac
     2581 gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
     2641 tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa
     2701 ctggcggcct ttattgacga atgccgtaac accaaagttg ctgaagctga aatggcgacg
     2761 atggagaaaa aaggcgtcaa tactggcttt aaagctgttc acccattaac gggcgaagaa
     2821 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agtaatggcg
     2881 gttccggggc acgaccaacg cgactacgag tttgcctcta aatacggcct gaacatcaaa
     2941 ccggttatcc tggcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
     3001 aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac
     3061 gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
     3121 cgtgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg
     3181 gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat
     3241 gtggtgatgg acggcattac cagcccgatt aaagcagatc cacagtgggc gaaaactacc
     3301 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
     3361 tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg
     3421 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
     3481 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
     3541 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
     3601 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
     3661 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
     3721 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
     3781 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
     3841 tggcaggaat ccggcgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg
     3901 tacgagcata ctgcgaaagg tgatgttgcg gcactgaacg ttgatgcact gactgaagat
     3961 cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc
     4021 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
     4081 aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt
     4141 gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa
     4201 ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa
     4261 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgctaaaat caccgttccg
     4321 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
     4381 tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg
     4441 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
     4501 cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca
     4561 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc
     4621 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
     4681 ttccatccct gcgtttgggt gctgtatcaa tctcacagga taccgcgtcg gttttccgta
     4741 atggtcaaac tgcggaatat cagatggtga tgactgttag cgcgtcggta ttgattcctg
     4801 gacgagatat ctacccgatt agcaccaaag tcttccgttc attcttcgat aacccgcaaa
     4861 tggcgttagc gaaagataac gagcaggaaa tgatcattaa agagatgtac gaccgtgctg
     4921 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
     4981 aacagacgtc gactacaacg gatactccgg caacacctgc acgcgtctcc accacgctgg
     5041 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
     5101 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt
     5161 tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa
     5221 cactgactgg aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca
     5281 aacgctattg ctgttgttac cagaaaacgg accgaacgcg gcgatcaatg aacaacttct
     5341 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
     5401 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
     5461 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct
     5521 caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct
     5581 gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac
     5641 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcactcctt ttcattgggt
     5701 tgatgctttg ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct
     5761 ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactct tgttactggt
     5821 taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt
     5881 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt
     5941 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacagtca
     6001 gtcagtgtgg gcagagttgg aagggttatc tcttctgttg tgccataaac ccctggcgga
     6061 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
     6121 cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
     6181 gagtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
     6241 tacagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
     6301 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
     6361 aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct
     6421 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
     6481 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
     6541 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
     6601 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcat
     6661 gtgatgattt attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct
     6721 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca
     6781 agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat
     6841 tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc tggttttatg
     6901 cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc
     6961 cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
     7021 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
     7081 tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat
     7141 cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt
     7201 acttagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg
     7261 cgttgaccag gggtgttgga gcgccattga tataaaccaa aaattcgcga cgctacgcgt
     7321 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
     7381 gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc
     7441 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
     7501 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
     7561 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
     7621 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
     7681 tcatcgcctt agacgttcag ggcaaatcca gcatcactga ctgcatgatc atctgtacgg
     7741 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
     7801 cgggcctgtt accgctcgga gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
     7861 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
     7921 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
     7981 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
     8041 tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca
     8101 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
     8161 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
     8221 gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
     8281 cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc
     8341 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
     8401 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
     8461 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
     8521 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
     8581 actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
     8641 gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag
     8701 aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
     8761 ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
     8821 tcacctccat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
     8881 atcagtaccg ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg
     8941 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
     9001 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg
     9061 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
     9121 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt cccaccgcaa gccggacacg
     9181 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
     9241 gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
     9301 cgcctagtta tgacccaaac ttgtttgttg acggtatctc cagcaaagat tattccgcct
     9361 tgttgaacga cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
     9421 cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca
     9481 atacgacgct gtttgaccct ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg
     9541 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
     9601 ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg
     9661 gtaaattcgg ttatggtcat tataccggta tcgacctggc ggaagaacgt tccggcaata
     9721 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca
     9781 ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac
     9841 tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg
     9901 aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgttggc gatattcatt
     9961 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta
    10021 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc
    10081 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    10141 gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca
    10201 tgattctgga gaacggtggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg
    10261 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcct gcggttgccg
    10321 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    10381 ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt
    10441 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    10501 gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    10561 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc
    10621 catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    10681 aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    10741 gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    10801 ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact
    10861 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt
    10921 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    10981 cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    11041 tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga
    11101 atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt
    11161 agtgggcatt ttgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat
    11221 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt
    11281 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    11341 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    11401 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    11461 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    11521 gcgatgatgg tcagcaacag acagtaagtg taccgcagcc tgcggtatgt aacggcccca
    11581 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    11641 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttatccagg
    11701 cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag
    11761 ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    11821 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt
    11881 acggcaacga ccgcgttatt tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa
    11941 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    12001 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt
    12061 taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    12121 gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagc agcggtttcc
    12181 tcggcgcacc aacgacctta gcgccaggtg tactggaagg cagcgaaccg acgcctgctc
    12241 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac
    12301 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaggtcggg gccgtaagcg
    12361 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    12421 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc aacaaagccg
    12481 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca
    12541 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    12601 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt
    12661 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    12721 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    12781 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    12841 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    12901 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    12961 aaatttaaag aaactgattt agtcactatt ggcaacgacg catgggccac cggtaacccg
    13021 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    13081 ctgatccgcg gtataaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    13141 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc
    13201 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    13261 gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg
    13321 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    13381 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    13441 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc
    13501 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    13561 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    13621 tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattccg
    13681 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    13741 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    13801 gaacaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    13861 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    13921 tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt
    13981 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    14041 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    14101 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    14161 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    14221 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    14281 ttctccgtta cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    14341 tcccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    14401 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    14461 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    14521 aagggcaggc aggaaaggcg gagcacattt taatgcccgg tgatattccg gtgattcaga
    14581 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtattgc
    14641 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    14701 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgccaggtg
    14761 tctatgttgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    14821 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    14881 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    14941 ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    15001 aatatattac cgcttaattt catacctcaa tggcccaatt tacactgggt cattactcat
    15061 aacacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    15121 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
    15181 attatgatga gca
//
LOCUS       DAZFKY010000112        15157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFKY010000112 DAZFKY010000000
VERSION     DAZFKY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            164..1222
                     /gene="citC"
                     /locus_tag="ACHKCL_003104"
     CDS             164..1222
                     /gene="citC"
                     /locus_tag="ACHKCL_003104"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5764019.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTESFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1237..1533
                     /gene="citD"
                     /locus_tag="ACHKCL_003105"
     CDS             1237..1533
                     /gene="citD"
                     /locus_tag="ACHKCL_003105"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5764020.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1530..2438
                     /gene="citE"
                     /locus_tag="ACHKCL_003106"
     CDS             1530..2438
                     /gene="citE"
                     /locus_tag="ACHKCL_003106"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5764021.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     ESVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2449..3981
                     /gene="citF"
                     /locus_tag="ACHKCL_003107"
     CDS             2449..3981
                     /gene="citF"
                     /locus_tag="ACHKCL_003107"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5764022.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3985..4536
                     /gene="citX"
                     /locus_tag="ACHKCL_003108"
     CDS             3985..4536
                     /gene="citX"
                     /locus_tag="ACHKCL_003108"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5764023.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4511..5389
                     /gene="citG"
                     /locus_tag="ACHKCL_003109"
     CDS             4511..5389
                     /gene="citG"
                     /locus_tag="ACHKCL_003109"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5764024.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5440..6903
                     /gene="citT"
                     /locus_tag="ACHKCL_003110"
     CDS             5440..6903
                     /gene="citT"
                     /locus_tag="ACHKCL_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5764025.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7017..7823
                     /gene="rna"
                     /locus_tag="ACHKCL_003111"
     CDS             7017..7823
                     /gene="rna"
                     /locus_tag="ACHKCL_003111"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5764026.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="ACHKCL_003112"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="ACHKCL_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5764027.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8601..8690
                     /gene="yldA"
                     /locus_tag="ACHKCL_003113"
     CDS             8601..8690
                     /gene="yldA"
                     /locus_tag="ACHKCL_003113"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5764028.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8694..9932)
                     /gene="ybdR"
                     /locus_tag="ACHKCL_003114"
     CDS             complement(8694..9932)
                     /gene="ybdR"
                     /locus_tag="ACHKCL_003114"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO5764029.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10153..10581
                     /gene="uspG"
                     /locus_tag="ACHKCL_003115"
     CDS             10153..10581
                     /gene="uspG"
                     /locus_tag="ACHKCL_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5764030.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="ACHKCL_003116"
     CDS             complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="ACHKCL_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5764031.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="ACHKCL_003117"
     CDS             complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="ACHKCL_003117"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5764032.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13331..14077
                     /gene="dsbG"
                     /locus_tag="ACHKCL_003118"
     CDS             13331..14077
                     /gene="dsbG"
                     /locus_tag="ACHKCL_003118"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5764033.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14287..>15157
                     /gene="ybdO"
                     /locus_tag="ACHKCL_003119"
     CDS             14287..>15157
                     /gene="ybdO"
                     /locus_tag="ACHKCL_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5764034.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFS"
BASE COUNT         3697 a         3946 c         3724 g         3790 t
ORIGIN      
        1 ttcacgccga aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatca
       61 cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg
      121 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      181 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      241 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      301 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      361 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      421 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      481 ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg atggtgctga tggaaaacag
      541 cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa
      601 gattggctgc attgcgatga acgccaatcc ctttacgaat ggtcaccgtt atctgattca
      661 acaagctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      721 cttcccctat gaagaccggc tggatctggt gttaaaaggc accgccgata ttccacgcct
      781 gactgtgcat cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat
      841 taaagaacag agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca
      901 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaat ccttttgtcg
      961 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
     1021 agcgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1081 ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1141 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1201 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1261 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1321 atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1381 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgataaa
     1441 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1501 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1561 ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact
     1621 ccttcatcta cccggctgat gccctgatgt ttgacctcga agagtccgtg gcattacgtg
     1681 aaaaagacac cgcccgccgc atggtttatc acgcgctgca acatccgctg tatcgcgata
     1741 ttgaaaccat tgtgcgtgta aacgcgctgg attccgaatg gggtgttaac gacctggaag
     1801 ccgtcgttcg cggtggtgcg gacgttgtac gtctgccgaa aaccgatacc gctcaggatg
     1861 ttctggatat cgaaaaagag atcctgcgta tagaaaaagc ctgtggtcgt gaacccggca
     1921 gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1981 ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc
     2041 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc
     2101 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2161 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2221 tgatcaaccc gcgtcagatt gatctgctgc acaacctcta cgcaccgacc cagaaagaag
     2281 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2341 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgtgcc cgtctggtgc
     2401 tctcccgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2461 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2521 cttgccgctt tccagaactc accaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2581 aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg
     2641 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2701 atcgcgaaga tgggctttaa aaacctgatc ctggcgtcca gctccctgag tgattgccat
     2761 gcgccgctgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctccggcctg
     2821 cgtggtccac tggcggaaga gatctcccgt ggtctgctgg cggaaccggt gcaaatccac
     2881 tctcacggcg gacgtgtgca tctggtacag agcggcgaac tgaatatcga cgtggctttc
     2941 ctcggcgtcc cgtcctgtga tgaattcggt aatgccaacg gctacaccgg taaagcctgc
     3001 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaaacaggt tgtgatgctt
     3061 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat
     3121 ttaatcgtca aagttgatcg cgttggcgat gctgcaaaaa tcggcgctgg tgctacccgt
     3181 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac
     3241 tcaggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc atcgctggcg
     3301 gtaacccttt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc
     3361 ggtggtatta ccgcgacgat ggttgatctg cacgaaaaag gtctgatccg caaactgctg
     3421 gatgtacaga gctttgacag ccacgctgcg caatcgctgg cccgtaaccc caatcacatc
     3481 gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gtgcatcggt tgatcgtctc
     3541 gacgtggtgg tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3601 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3661 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgactct ggtggataac
     3721 gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggtatc
     3781 gcagttaacc cggcacgtcc ggaactggca gaacgtctgc aggaagcggg cattaaagtg
     3841 gtttccattg agtggctgcg cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt
     3901 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3961 gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     4021 gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgtctgg
     4081 ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa
     4141 gacagtgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4201 gcaaaacagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4261 ggtatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4321 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt
     4381 ctctcccgcc gcgactattc actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca
     4441 gccgtctgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggca
     4501 ctgctgaacg atgtcgatgc ctgcaacgtt aactaaaacc acaaagcttg cgacgtcatt
     4561 aatcgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca atctgtcacc
     4621 gaaaccaggt ctcgtggatc gcattaactg cggtgcgcat aaagatatgg cgctggaaga
     4681 tttccaccgc agcgcgctgg cgattcaggg ctggctaccc cgtttcattg aatttggtgc
     4741 ctgtagtgcg gaaatggcac cagaagcggt actccacgga ttacgcccaa ttggtatggc
     4801 ttgcgaaggt gatatgttcc gcgccaccgc gggcgtaaac acgcataagg gcagcatttt
     4861 ttctttaggg ctactgtgtg cggcaattgg ccgtttgctt cagctcaacc agttgctaac
     4921 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggcctgaccg atcgcgaact
     4981 gcgtaccaat aattcacaac tgacggcagg tcaacggttg taccagcagc ttggccttac
     5041 cggcgcacgc ggtgaagccg aagcgggtta tccactggtg atcaaccacg ccttgccgca
     5101 ttacctcact ctgctggatc aggggttaga tcctgaactg gcattgctcg ataccttgct
     5161 cctactgatg gcgatcaacg gcgataccaa cgttgcttcg cgcggtggcg aggggggcct
     5221 gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc
     5281 agatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5341 cggcagtgcc gacctgctga tccttacctg gtttttagca cagatttaat tatttaagaa
     5401 cttgataaat ttggaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5461 atatggaaac tattggcccc actggtggtg atgggtgtca tgtttcttat ccctgtcccc
     5521 gacggtatgc cgccgcaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
     5581 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac tatttgcgtt
     5641 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5701 caaaaacagg cgctgaaatg gggcctggct ggtttttcca gcactacggt atggctggta
     5761 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
     5821 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
     5881 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5941 tttccggtca ttaaaaacct gccgccgttg tttaaatcat tcccgaacga tccgtccgcg
     6001 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6061 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6121 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6181 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6241 gaagtggcaa cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac acgcagagag
     6301 tggacgctga ttggccttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc
     6361 attaatgcta ctgcggttgg tctgctggca gtttcgctaa tgctggctct gcacgttgtg
     6421 ccgtggaaag acattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact
     6481 ctggttgtga tggctaacgg cctgactcgt tctggtttta ttgactggtt cgccggtacc
     6541 atgagtacgc acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg
     6601 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6661 gttattctgg ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg
     6721 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     6781 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6841 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     6901 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggttagcc cacctggcgg
     6961 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     7021 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7081 acgccgtagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7141 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7201 gtcgcctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     7261 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7321 gttgcgctac tcgcccaatc ccgaatctac cagaagcgcg cgccagccgg atgtgttcat
     7381 cgccggaaac cggattatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     7441 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7501 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagagata aaagagagcg
     7561 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     7621 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7681 gtaaccctgc gtatttgact gaaattcagg tctcgatcaa agctgacgcc atcaacgctc
     7741 cgctttctgc aaactcattc ttgccacaac cacacccagg taactgtggc aaaacctttg
     7801 tgattgataa agcgggttat taatcctctg atttacaaaa cacgctaatt cgtctttcat
     7861 aaaccatgac gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg gactgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8461 taatcccatc tgtacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 atgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgctcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgcttttc aaagatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     9001 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactgacgc
     9061 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     9121 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     9181 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
     9241 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     9301 gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccggc accatagaca
     9361 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
     9421 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     9481 acattccctt tagggacacg gacatactcc gcctgtccac caggaacgcc accatacagg
     9541 tgactataac caaacaatgc cgcgggagct ggtatctgtt ttttattgag cgcagcgcct
     9601 ttacccgcat tggtattttc gcaggcggca tattgctgca gtcgacagaa aaaacagtcg
     9661 ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacatcc
     9721 tttccggttt caactacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
     9781 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgttata
     9841 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
     9901 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
     9961 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
    10021 aattcctggt gttcagtgag aatatattca tcacagaaac agttcgtaag gaaaaagaac
    10081 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
    10141 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
    10201 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
    10261 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
    10321 cgttttgaag agcatctgca acatgaagca gaagaacgtt tgcaaacgat ggtcagccac
    10381 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
    10441 gtcaatgagt tagcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
    10501 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg
    10561 cctgtactgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc
    10621 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
    10681 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
    10741 cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt
    10801 acagtcaccc gctgcgaaca cgcctttcac gttggtttcg catttcgcat caatgataat
    10861 ctcgcccatg cggttacgtt cgactgcgcc ttcgagccag ttggtgttcg gcagcagacc
    10921 aatctgcacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatctcg
    10981 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
    11041 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
    11101 ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt caattgccgc
    11161 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttacctttaa acagcgggcc
    11221 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc ccggaacgtt
    11281 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
    11341 agaagctgtt tcaatctgat gcagaccacc ttcaactgct gctgggatca gtttgctggc
    11401 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
    11461 cagcttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
    11521 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
    11581 aattgctgcc gctgcacccg ccgggccgga accgacgatt aatacgtcat aagcatcacg
    11641 cttgttcagc tcttctgccg cacgtttttc cgcgcccgta tcaattttgg caacgatttc
    11701 agtcaaagtc atgcggccct gaccaaactc tttcccgttc acgaacactg ccggaacgcc
    11761 catcacgtta cgatcagtga tctcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
    11821 gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgt accacgtccg ggcagttatg
    11881 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
    11941 cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagtaacg ccagtaccag
    12001 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tagaacctgg
    12061 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
    12121 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
    12181 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
    12241 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
    12301 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
    12361 gccgcagcac ccgattaatt acgcgattgc ggaacttaga ttttaccaac caggtccaga
    12421 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    12481 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg
    12541 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    12601 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    12661 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    12721 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
    12781 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    12841 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    12901 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    12961 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttaccg attcggctaa
    13021 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    13081 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    13141 atctgaacta ccctttggta tcttcagcgc cagtctgcag gctgcgttgt tgctcttaca
    13201 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    13261 gctcttacct atgacaatga taggttatgc cttttactcg acttttgcac tgactgaaaa
    13321 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    13381 agaggaactt cctgctccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac
    13441 gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
    13501 catctacctg actccagatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
    13561 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
    13621 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
    13681 tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
    13741 cccgtgggta gattctggca aagtacagtt gagaacattg ttggttgggg ttatcaaacc
    13801 agaaagtccg gcgacagccg cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    13861 agaatatgaa gcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
    13921 gcaaatgaaa gtgttaagcg acaacgagaa actcatggac gatctggggg caaatgtcac
    13981 tccggctatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
    14041 tcagaaaacg cttaatatca ttatgggaaa taaataaaag gtaattcatg gatggttgaa
    14101 ctatatccct gcttttcaac catcctaaaa aagcatgaat tgctcgctct gccctaagtg
    14161 gctgtgctat tttagaaaaa cttaatcctg agttaagcaa aaaaaacata atcaataaaa
    14221 atatatacgc aaaaaacatt gattaagtga atatatcatg gaagaaaaat ataaccggag
    14281 tagtgtatga ccaatctcta cgacttgaaa aagttcgact taaatcttct tgtcatattt
    14341 gagtgtattt accagcattt aagcattagc aaagctgctg aatcactata tatcacccca
    14401 tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc tttattcatt
    14461 cgctcaggaa aaggtatcgc gccgacgaca acagggttaa atctgcatca ccatcttgag
    14521 aaaaatctca gaggccttga acaaacaatt aatatagtca ataagtccga actaaagaaa
    14581 aactttatta ttcacggccc tcagctcatt tcttgctcta ataatagtat gctgatccgc
    14641 tgcctacggc aagattcctc tgtcgagatt gagtgccatg atatcctcat gtcagcagag
    14701 aatgctgaag aactgttggt tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg
    14761 atcagccgtt ccgtcatttg catgccattg catacgattc gcaatactct aatttgtagt
    14821 aataggcatc cacgaattac tgacaatagc acttatgaac aaattatggc agaagagttc
    14881 acccaattaa tatctaaatc agcaggggtt gatgatattc aaatggaaat agacgagaga
    14941 tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaatcat taatagcatt
    15001 gcagttacag atttactggg aatagtcccc tatgaacttt ataactccca ccgtgacttt
    15061 cttaatttaa aagagataaa actagaacat ccgttacctt caataaagct ttacatttcc
    15121 tataataaat cttctttaaa caatctggtg ttctcac
//
LOCUS       DAZFKY010000113        15085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFKY010000113 DAZFKY010000000
VERSION     DAZFKY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..552
                     /gene="yceJ"
                     /locus_tag="ACHKCL_003120"
     CDS             <1..552
                     /gene="yceJ"
                     /locus_tag="ACHKCL_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5764035.1"
                     /translation="TPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGWYHKAP
                     ELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLFAIGIS
                     GYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHGFMALK
                     HHFIDKDDTLKRMLGKSSSDYGV"
     gene            556..1131
                     /gene="yceI"
                     /locus_tag="ACHKCL_003121"
     CDS             556..1131
                     /gene="yceI"
                     /locus_tag="ACHKCL_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5764036.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1173..2225)
                     /gene="trhO"
                     /locus_tag="ACHKCL_003122"
     CDS             complement(1173..2225)
                     /gene="trhO"
                     /locus_tag="ACHKCL_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5764037.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2450..3370
                     /gene="lpxL"
                     /locus_tag="ACHKCL_003123"
     CDS             2450..3370
                     /gene="lpxL"
                     /locus_tag="ACHKCL_003123"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5764038.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3542..4768
                     /gene="mdtG"
                     /locus_tag="ACHKCL_003124"
                     /pseudo
     CDS             3542..4768
                     /gene="mdtG"
                     /locus_tag="ACHKCL_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            4851..5225
                     /gene="msyB"
                     /locus_tag="ACHKCL_003125"
     CDS             4851..5225
                     /gene="msyB"
                     /locus_tag="ACHKCL_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5764039.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5226..5453)
                     /gene="yceK"
                     /locus_tag="ACHKCL_003126"
     CDS             complement(5226..5453)
                     /gene="yceK"
                     /locus_tag="ACHKCL_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5764040.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5626..8169)
                     /gene="mdoH"
                     /locus_tag="ACHKCL_003127"
     CDS             complement(5626..8169)
                     /gene="mdoH"
                     /locus_tag="ACHKCL_003127"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5764041.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8162..9697)
                     /gene="mdoG"
                     /locus_tag="ACHKCL_003128"
     CDS             complement(8162..9697)
                     /gene="mdoG"
                     /locus_tag="ACHKCL_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5764042.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10090..11247
                     /gene="mdoC"
                     /locus_tag="ACHKCL_003129"
     CDS             10090..11247
                     /gene="mdoC"
                     /locus_tag="ACHKCL_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5764043.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11255..12736)
                     /gene="clsC"
                     /locus_tag="ACHKCL_003130"
     CDS             complement(11255..12736)
                     /gene="clsC"
                     /locus_tag="ACHKCL_003130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5764044.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12678..13211)
                     /gene="ymdB"
                     /locus_tag="ACHKCL_003131"
     CDS             complement(12678..13211)
                     /gene="ymdB"
                     /locus_tag="ACHKCL_003131"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5764045.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13306..13617)
                     /gene="ymdA"
                     /locus_tag="ACHKCL_003132"
     CDS             complement(13306..13617)
                     /gene="ymdA"
                     /locus_tag="ACHKCL_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5764046.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13738..14070)
                     /gene="csgC"
                     /locus_tag="ACHKCL_003133"
     CDS             complement(13738..14070)
                     /gene="csgC"
                     /locus_tag="ACHKCL_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5764047.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14129..14587)
                     /gene="csgA"
                     /locus_tag="ACHKCL_003134"
     CDS             complement(14129..14587)
                     /gene="csgA"
                     /locus_tag="ACHKCL_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5764048.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(14628..15083)
                     /gene="csgB"
                     /locus_tag="ACHKCL_003135"
     CDS             complement(14628..15083)
                     /gene="csgB"
                     /locus_tag="ACHKCL_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5764049.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3758 a         3867 c         3611 g         3849 t
ORIGIN      
        1 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
       61 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
      121 cacaaagcac ccgaactaca taaaagcatc ggtattttgt taatgatggg gctggttatt
      181 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
      241 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
      301 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
      361 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
      421 ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag
      481 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
      541 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
      601 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
      661 gttaactttc gcatccagca tcttggctat agctggttat atggcacctt taaagatttc
      721 gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt
      781 aacaccacca gcgtcgatac taatcacgcc gaacgtgata aacatcttcg cagtgcagat
      841 ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
      901 ggtgacgaac tggatattac cggcgatctg acgctgaatg gtgtaaccaa acccgtcacg
      961 ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc
     1021 gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct
     1081 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat
     1141 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt
     1201 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
     1261 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ttcactacaa
     1321 caacctttgt atttttccgc acatactgga cactgaataa acagcaggtg gcagccatca
     1381 tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc
     1441 tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
     1501 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccacc ctcgatatgc
     1561 cagactttat tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
     1621 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
     1681 ggcagctgct cacggaaggt atctgccgga atttccagcg cgttttcaaa gtgccccact
     1741 tcatactcat agtggttacg catgtcgata aacagtgcat cgggatcgtc aagcatggcg
     1801 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
     1861 ataccgtcgg caacgatacg atcgcggacc ttcatgcgca gtacccagaa ggatttcccg
     1921 tcatcatcca acgcgatatt caggcgtaag ccatctaaag ccgagtcgaa ggcatagagc
     1981 tgcgcgcgaa atgttttaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
     2041 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
     2101 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
     2161 tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact
     2221 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
     2281 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
     2341 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
     2401 atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct
     2461 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
     2521 cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt
     2581 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
     2641 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
     2701 atccgttggc atgggactga tggaaactgg catggcgtgg ttctggccgg accgccgaat
     2761 cgctcgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
     2821 cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg
     2881 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
     2941 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
     3001 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg
     3061 cccgcgctca agcgttttcg tccctttatt tgccgttgag caggctgcga ccacgaccgg
     3121 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
     3181 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccgctgga
     3241 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
     3301 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
     3361 acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa
     3421 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
     3481 ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
     3541 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
     3601 gctaggctgt tttcttaccg gtgccgcctt cagtctggta atgcccttct aacccctcta
     3661 cgttgagcag cttggcgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt
     3721 cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg
     3781 taaaggccga aaactcatgc tattacgctc tgctctcggc atgggcatcg tgatggtgtt
     3841 gatggggctg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttgggttact
     3901 tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag
     3961 cggctgggca ctgggtacgc tctccacagg cggcgttagc ggtgcgttgc tcggcccaat
     4021 ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag
     4081 tgtgctcata ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt
     4141 cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact
     4201 ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacgggct caattgcccc
     4261 cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag
     4321 tggcatgatc gcctcggtgc caggcgtagc tgctctgctg agtgcaccac gactcggcaa
     4381 acttggcgat cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact
     4441 gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt
     4501 gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc
     4561 gagcaaccaa atcgccggac gtatcttcag ctataaccaa tcgtttcgtg atattggcaa
     4621 cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt
     4681 tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg
     4741 tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga
     4801 atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt
     4861 acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg
     4921 gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg
     4981 acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt
     5041 tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag
     5101 agatccgcca ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc
     5161 agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac
     5221 gttaattact catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc
     5281 agggagaatg gcagatcaag gattgtgaca taacgccagg cggagtcacg cacatcccat
     5341 tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg gctaatgatg
     5401 ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac
     5461 ctcttccgta aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc
     5521 agatgtggcg tgaacgcctt atccgaccta caaaatcttg caaaatcaac acattgcagg
     5581 agcgatgtag gcctgataag catagcgcat caggcaacta cgtttttatt gcgaagccgc
     5641 atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga
     5701 agaatatctc tcaggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc
     5761 gcttagcagc acaaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg
     5821 ttcaacatgg cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc
     5881 cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa aaccatcatc
     5941 aagggagcgt tgacggttca tctcaaggaa ccgatcggta tcaaccagta cctgcggcgg
     6001 tgaatactct tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc
     6061 acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag
     6121 ccagaacagg aaacgcagat ccaaccacgc catcccgaca gcccacacta accctaacag
     6181 cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg
     6241 cggtgaattc cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa
     6301 cagcatgcgt accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt
     6361 aacgcgccag aagccgccat attctttcgt tcctttacac caaataagca aaatgctcaa
     6421 tagctttggc aggaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg
     6481 acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt
     6541 caacgcatgc actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc
     6601 ggagagataa gacatcacgc ccgtcaggaa taccgcacgg tgaaccgggt gcataccctt
     6661 caccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag
     6721 ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca
     6781 gactccccaa cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg acaggattga
     6841 accggcaaag gaaccttcgc ccggcagcgg ggccagtgca cagtgctcga taaacggttt
     6901 cacgcggata atcgcgttat gcccccagta gtgcgactcg ccaagttgcc agaagtgcaa
     6961 accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gtgcatacag
     7021 cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc
     7081 catcaggcgc accaggccgc acaaacaatc accggtcatt accgagtcag catccagcac
     7141 caccatatag ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt
     7201 acgcttcacg cgacggcggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc
     7261 gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttgt aactgtcact
     7321 cagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc
     7381 acgcaagcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt
     7441 gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc
     7501 gcgaccaata agtagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca
     7561 acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg
     7621 cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc
     7681 ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag
     7741 gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg
     7801 ctcttctttg gtcaaacgag ccagatagcg cggtgttaca tcgcgtccac gcaggcgatc
     7861 ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc
     7921 ttctggcatc gccttaagct gatcgcgccc ttcgtcatct ttaattaact gtccatcagc
     7981 aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg gggagtcatc
     8041 ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt
     8101 cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt
     8161 cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca
     8221 ccagcgcagc gcgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca
     8281 gacgccagcc tttggtgacc gggttatagc gcaccgtgct ttcaactatc tcaccattat
     8341 caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc
     8401 cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct
     8461 tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt
     8521 tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg
     8581 gcagctgatc cggcgtccag taagcgacga tgttatcgtt agtttcatcg ttggttggaa
     8641 tttccaccag ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg
     8701 gacggagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca
     8761 acagaccaaa gccttgcggg ttttccatgg agaagctgct gaccgctaaa tgtttcgggt
     8821 tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt
     8881 cgtgcaactc cggacgatag ttatttgctg gcgacggttg gttcggccca aacaggaaca
     8941 tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact
     9001 gcacatccac aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc
     9061 gcggcgagtc aagcaatgca taaatggtta aacgtttatc agttggtttt ggacgctcga
     9121 tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca
     9181 gaccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc
     9241 cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa
     9301 aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga
     9361 aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga
     9421 ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat
     9481 tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat
     9541 cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt
     9601 gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta
     9661 acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta
     9721 gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc
     9781 cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga
     9841 ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg
     9901 gtttgccagc aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta
     9961 gcgatgatgc tatgcataaa aacttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc
    10021 tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt
    10081 taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc
    10141 tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg
    10201 catgtgaata gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt catccactcg
    10261 ttccgcatgc aggtattttt cgttatatcc gggtactttt cctacatgct ttttttacgc
    10321 tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg tagggatccc gatgttaaca
    10381 gccatccccc tactgacatt gccgcaattt attatgctgc aatacgtcaa agggaaagcg
    10441 gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata
    10501 tcacatctgt ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt atggatattt
    10561 aaacgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta
    10621 aaactatcgg tgattttttt gtgcctcggc atcggttatg cggtaataag aagaacgatt
    10681 tttattgtgt atccacccat tctgagtaat ggcacgttca attttattgt catgcaaacg
    10741 ctattttatt taccgttctt tatcctcggc gcactggctt tcattttccc tcatcttaaa
    10801 gccttgttta ccacgccgtc tcgtggctgt accctcgctg cagcattggc gtttgtcgct
    10861 tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg
    10921 atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg ccaccgtttg
    10981 cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt tatctatctg
    11041 gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat cacctccaac
    11101 tggcttggtt ttctctgtgg cctgatattc gtagtaggga ttgcgataat tctgtatgaa
    11161 attcatttgc gcatcccgtt actgaagttt ttgttctctg gtaaaccggt tgttaagcgt
    11221 gagaacgata aagcaccagc ccgttaagcc acatttacaa taaccattcc acgggcaata
    11281 tcgacgccag tctgaccata acccgcttcc agaaactggt agcgggttct tttttgagaa
    11341 caatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg tccagacgga
    11401 gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac tgagccagcg
    11461 tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc ggatcgaaat
    11521 tgaaagaacc gataaacacc gttttaccat cgatgctaaa ggttttagca tgcaggctgg
    11581 caccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca cgcgtcggct
    11641 tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg
    11701 catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca atctttaccc
    11761 cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca aaataggaag
    11821 agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca
    11881 gtgaatgccg ttttgccttg ccctcccctt tcgccggatc atcacttaat aaacgtgttt
    11941 tcgcccagat aagcggcaac gttccatcaa ccagatgatt tataaacgga ctggattcca
    12001 ttttgcgtaa gtaacgatgc gtcatggcat cgttatgcca ggaggcgggt aactcgatgc
    12061 gatccgccat ttcaccttcc gggacatcca gcacctgctg taagggtgaa accgatttgc
    12121 aataccagta gcgggcgaaa tcatcggcaa cgtcctctac tacgggtcct attgccatga
    12181 catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa ataggcatca ccaatatttc
    12241 gcccccctac caacgtcacc acgccatcga cagtgaaact tttattgtgc atacggcgat
    12301 tgagacggga aaagtcggtg atataaccaa gcggacgcag caagcgaaac gagaaaggat
    12361 taaaaagccg gacttcaatg cggggatggc tgtcaagcaa gcgtaaaatg tcgtcaagtc
    12421 cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt gctgcggcta
    12481 acagggcgga aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa tactgaacat
    12541 ccagcgtatg ctctgacatt tcggccagac gataccgggc ggcaaacgca tccagacttt
    12601 tctccagagg gaaaagacca cactgaccgg gatgttgcga acacagtggc agcaccgcgc
    12661 tcgccagccg gggcaaatca ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg
    12721 gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg acgggtaata
    12781 aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta accataaacc
    12841 ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgccgccac cagtcgtagg
    12901 ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc acgccagact
    12961 ggcccgacgg tgtgcactac ggctttagca ggaagatcgc ctgcaagcgt aataacggca
    13021 tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta aacaagcatc cagcagggcc
    13081 ggacccgctg cgcgatgaat ggccccatcg acgccgccac ctcccattaa tgaaggatta
    13141 gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac aacatgaata
    13201 cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc aggctttttg
    13261 gttaaatcgc atttttctgc tcagcaacgt gagttgttca gaaacttagt tatattcgat
    13321 gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg aggctatttg
    13381 cgcgtttttg acattgccgc tcattaacgc tttgctggag taggttttac ccggaacaga
    13441 accattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt cacatgccgg
    13501 ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc ctgcgatggc
    13561 aataaaagga aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc tgaacattta
    13621 ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact ttcaggttgg
    13681 ctgattcgca gcagaccatt ctctccagat tcatcttatg ctcgatattt caacaaatta
    13741 agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc catcagaaac
    13801 agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc
    13861 aatgggttga ttagcaggca atgaaagggt cttttcttgc ttcgtctgac tttgccctga
    13921 actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag taagagtgac
    13981 ttcaggaata atggtgtaca tatccccttg ctgggtcgta ttaaaggtta tctgactgga
    14041 aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc
    14101 gccctgtttc tttaatacag atgatgtatt agtactgatg agcggtcgcg ttgttaccaa
    14161 agccaacctg agttacgttg acggtggaat tagatgcagt ctggtcaacc gctgcaccgt
    14221 tgccgccacc gaattgttta actgtcatat gagagtcttt accgttccac tgatcaagag
    14281 tggcgctgtt accaaagcca cgttgggtca gatcgattga gctgtcatct gagccctgac
    14341 caacatctgc accgttacca ccaccatgct gggtaatagt aagatcagag ttacgagcat
    14401 cagcttgcag agcaagtgca gagttaccac caccgtactg ataaatattc agctctgaat
    14461 tcgggccgct gttattaccg ccaccaccgt ggttaccgcc accgccgccg tactgaggaa
    14521 caacacctgc cagagcgcta ccggagaata cgattgctgc aattgctgct acttttaaaa
    14581 gtttcatgta aaacccccat cggattgatt taaaagtcga atggaaatta acgttgtgtc
    14641 acgcgaatag ccatttgcga ctgtctctgc actacaattg ccgttttttg agtaccatac
    14701 tgtgtaatat ttgctttatt accagaacct ttctggataa tcatcgcagt attaccataa
    14761 gcaccttgcg aaatactggc atcattggca ctgcccgcct gatcaatata tgcaaggtta
    14821 taatctcctg tctggtcaat ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc
    14881 aaaagttttg agcctccctg ccgtaactga gcactattat tagtcccagc ttgaccaatt
    14941 atggctgcct gattaaatga agacttactc aattcattta ccgcgaagtt atattctgaa
    15001 ttagctaaat cataacctgc tgcggctgca atcccaggcg cacccagtat tgttaacatc
    15061 ataaataaca atttgttttt catgt
//
LOCUS       DAZFKY010000114        15072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFKY010000114 DAZFKY010000000
VERSION     DAZFKY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(467..1558)
                     /gene="ychF"
                     /locus_tag="ACHKCL_003136"
     CDS             complement(467..1558)
                     /gene="ychF"
                     /locus_tag="ACHKCL_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5764050.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1675..2259)
                     /gene="pth"
                     /locus_tag="ACHKCL_003137"
     CDS             complement(1675..2259)
                     /gene="pth"
                     /locus_tag="ACHKCL_003137"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5764051.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2537..2815
                     /gene="ychH"
                     /locus_tag="ACHKCL_003138"
     CDS             2537..2815
                     /gene="ychH"
                     /locus_tag="ACHKCL_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5764052.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2870..4549)
                     /gene="dauA"
                     /locus_tag="ACHKCL_003139"
     CDS             complement(2870..4549)
                     /gene="dauA"
                     /locus_tag="ACHKCL_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5764053.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4674..5621)
                     /gene="prs"
                     /locus_tag="ACHKCL_003140"
     CDS             complement(4674..5621)
                     /gene="prs"
                     /locus_tag="ACHKCL_003140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5764054.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5772..6623)
                     /gene="ispE"
                     /locus_tag="ACHKCL_003141"
     CDS             complement(5772..6623)
                     /gene="ispE"
                     /locus_tag="ACHKCL_003141"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5764055.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6623..7246)
                     /gene="lolB"
                     /locus_tag="ACHKCL_003142"
     CDS             complement(6623..7246)
                     /gene="lolB"
                     /locus_tag="ACHKCL_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5764056.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7460..8716
                     /gene="hemA"
                     /locus_tag="ACHKCL_003143"
     CDS             7460..8716
                     /gene="hemA"
                     /locus_tag="ACHKCL_003143"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5764057.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8758..9840
                     /gene="prfA"
                     /locus_tag="ACHKCL_003144"
     CDS             8758..9840
                     /gene="prfA"
                     /locus_tag="ACHKCL_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5764058.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9840..10673
                     /gene="prmC"
                     /locus_tag="ACHKCL_003145"
     CDS             9840..10673
                     /gene="prmC"
                     /locus_tag="ACHKCL_003145"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5764059.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10670..11062
                     /gene="sirB2"
                     /locus_tag="ACHKCL_003146"
     CDS             10670..11062
                     /gene="sirB2"
                     /locus_tag="ACHKCL_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5764060.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            11066..11875
                     /gene="sirB1"
                     /locus_tag="ACHKCL_003147"
     CDS             11066..11875
                     /gene="sirB1"
                     /locus_tag="ACHKCL_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5764061.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11911..12765
                     /gene="kdsA"
                     /locus_tag="ACHKCL_003148"
     CDS             11911..12765
                     /gene="kdsA"
                     /locus_tag="ACHKCL_003148"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5764062.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12913..13020)
                     /gene="ldrA"
                     /locus_tag="ACHKCL_003149"
     CDS             complement(12913..13020)
                     /gene="ldrA"
                     /locus_tag="ACHKCL_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5764063.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13449..13556)
                     /locus_tag="ACHKCL_003150"
     CDS             complement(13449..13556)
                     /locus_tag="ACHKCL_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5764064.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13955..15055)
                     /gene="chaA"
                     /locus_tag="ACHKCL_003151"
     CDS             complement(13955..15055)
                     /gene="chaA"
                     /locus_tag="ACHKCL_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5764065.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3784 a         3808 c         3874 g         3606 t
ORIGIN      
        1 ttttgcatta ctaataagaa aaagcaaaaa acatcgatat tcatttttgg tcaatgggtt
       61 ttctggtcta tcgttgttat tgtgctactg gttatcattt tcatcttgcc aggatcgcac
      121 gtaatcacgt tattcgggga ctttatctgc gggatattac ccttcaggta agtccttttc
      181 tcgccagcat attggagcgt ggtcaatatt gatcggctgt gaagggggct gatgtgtagt
      241 ggaacaaaaa ctgacgttat atcactgcta atgagatatc cgtcatctga acactgatgt
      301 ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg atgttacaag
      361 aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt atatactttt
      421 gcgctcttca tgagatttag cgaaacctca tgagacaata aattaattag acgttgaaaa
      481 ggaagttcat cacatcgcca tctttcacga tgtaatcttt accttctgca cgcattttgc
      541 ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac gagatggttt
      601 gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc tgcggcgcgg
      661 ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg aagtaagttt
      721 gcaggttcag cagcttataa ccggcacgga tcacacggtt cagacccggc tcttccagcc
      781 caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg tctgcttcaa
      841 cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca cgcacctggt
      901 caagatatgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac attgttggtt
      961 tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt aaatccagcg
     1021 cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttccaggacc gccagctcag
     1081 ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc gcacgttcgc
     1141 aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg tcagccgggt
     1201 taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca acgtgaccga
     1261 tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg cctttcgatg
     1321 cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc gtacgttgcg
     1381 gttttacgat ttcagccagt tgatccaggc gaggatcagg cattggtacg acgcctgtgt
     1441 tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg gtcagcgcgt
     1501 tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg aatcccatga
     1561 tttaaatcac cttaatatct taataatcaa cctgttattg ctaacagatt gcagaaatgg
     1621 aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa cgacttattg
     1681 cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa accacatttc
     1741 agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac taacaggcgg
     1801 tttgcctaac acaaaaccaa caactttgtt tttatcgccc ggatgaccga ttccgatgcg
     1861 taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca gtccattgtg
     1921 accaccatgg ccaccgccca acttaaattt ggcgacgcca ggaggcagat ccagttcgtc
     1981 gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg ccgcaacggc
     2041 tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt cgcctccaag
     2101 ggtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag cgcgcaaacg
     2161 ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg cgtattcagc
     2221 accggggttc gccaggccga caatcaattt aatcgtcacg tttttttgtc ctgagtgtgt
     2281 acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact gcgtatcaaa
     2341 tgcagataac gtaataattg cctgagtgga ctattagaaa gtcaaggtgt tcaggcgttt
     2401 atttgtaaag ttttgttgaa ataagggttg taattgtgat cacgcccgca cataacccac
     2461 agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac aacccagaaa
     2521 gttccggagg tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg tgctcatggg
     2581 gttgggtctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc agttaccaca
     2641 gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg tcggtgccat
     2701 tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc gttactggtg
     2761 ggttcgccac tatgacaaac gttgccgccg tagcgataat cgccgtcata gctaacaaat
     2821 aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact tataaatccg
     2881 ccatcgccgc gcgacgattc gggaagaacg cgaggcgtcc cgggatcggt tgaatgcctg
     2941 cgcgagccat agtgcgcagt ggctggaatt ccacgttgca cacgcgcagt tcacatcctt
     3001 cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca agtaccggaa
     3061 cggcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc aggtccgtga
     3121 ataagccttc agcagcagca aaaaacagcg ggccaataac gcgcagaacc aggacatcgt
     3181 ctggaacatc tacgactacc ggtgccaggc gagtcatacg tgcgatacga cgcataaaca
     3241 gcagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac agcacggtca
     3301 gcgacatgca cagcagcatg acaatgatgt catctttcgg tgcatgacgc agcaagtcga
     3361 ccactttatg cgcttcactc atgttccacg ccaccatcaa cagcagggct gccatagcgg
     3421 aaagcggcag ccaggagagc agcggtgcca gtaccagcag ggcaagaata accagaatag
     3481 agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg gcagaacgcg
     3541 cgatggcagc ggtagcggta ataccaccaa agaacggagc gatgatattc cccagcccct
     3601 gtccaaccag ttcgctattc gccttgtgct tcgtcccggt cataccatcc agcaccacgg
     3661 cgcagagcag agattcgatt gcgccgagca ttgccattga gaatgccgca ggcagcagtg
     3721 tgcgaataga atcccaggtt agcgtgaatt ctgaattagg cagatcccac ggcagcacca
     3781 gttgcggtag cagttgcgga ataccgttac cctgagaacc atcggccagg acgtagtgga
     3841 attgcgaacc gatggtagca acatgtccgc cgagcaggtt aacaatcccc atcaccgcgc
     3901 aaccagccag caaggccgga aggtgaccgg gtaaacgaat gcccagacgc ggccagaaaa
     3961 caagaatacc tagcgtcaca atgccaatgg cagcatcacc cacattaatg gtcggcagcg
     4021 ccataaataa tgcgccgact ttttgtagat aatgttccgg gacatgggcc atttgcagac
     4081 cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag gtgaaaccta
     4141 aggtgacgga aaccggaata tactcaatca ggcgaccaaa gcgtgccaga cccatcagaa
     4201 tcaaaaagat ccccgacagc aaggtcgcaa ccagcagtcc tgccagccca aactgttgcg
     4261 aaacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta aagcgtgacc
     4321 cacccgtcag agcaatgaca atccccgcaa cagctgcggt atataaaccg tactggggtg
     4381 ccacaccact accaatagcc aacgccatcg ccagcgggat agcaataatc ccgacggtta
     4441 tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc cagcaagcgt
     4501 cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca ataatgtttc
     4561 atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag gtcatacaaa
     4621 tggatattac agacaaaaaa acccgccgca gcgggtcttt gagccgggtt cgattagtgt
     4681 tcgaacatgg cagagatcga ttcttcgttg ctgatacgac gaatcgcttc ggccagcata
     4741 cctgacaggg tcagagtacg cacgttcggc agtgatttga tttcatcgct cagcggaatg
     4801 gtatcgcaga caacgacttc atcaattaca gagttacgca ggttgttcgc cgcgttgcca
     4861 gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag caccacgttc tttcagagct
     4921 tcagcagctt tacacagcgt accgccagtg tcgatcatat catcgaccag tacgcagtca
     4981 cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg cggacgacgt
     5041 ttgtcgatga ttgccatatc ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca
     5101 acgccgccga tgtccggaga aaccacaatt gggttatcca gattcagctg cagcatgtct
     5161 tccagcagga tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc
     5221 tgttcagcgt gcagatccac tgtcagcaca cggtcaacac cgacgctgga gaggaagtct
     5281 gcaaccactt tcgcagtgat tggtacacga gcggaacgga cgcgacggtc ctggcgcgca
     5341 tagccaaagt aggggataac agcggtgata cgacctgcgg aagcacgacg cagggcatca
     5401 accataacga ctaattccat caggttgtcg ttagtagggg cacaagtgga ctggatgatg
     5461 aaaatatcac caccgcgtac attttcatta atttgtacgc tgacttcgcc atcgctaaag
     5521 cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt
     5581 tccggggtgg cgttaccagc aaaaagcttc atatcaggca cgagaagaac ctcaggcatg
     5641 cgtccattgg tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat cctttccagg
     5701 cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca
     5761 gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa gattagcgcc tttcgccaca
     5821 aagccattga gccattccgg ggcttgctct agcacctggc gggcttcaga ctctgtatca
     5881 aattcagcaa agacacaggc ccctgtccca gtcaggcgcg acggggcgta ttctaacagc
     5941 caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc gcaatcattg
     6001 ctgaattcac attttagcaa cgtttctatt gacctttttg gcgtattgcg cgggagttca
     6061 ggatctttaa aaatcaccgg agtcggaata cttacaccag ggtgcgccac cagataccac
     6121 ttctctggcg gatccaccgg ggttagtatt tcaccaacgc cttcggcaaa cgcggcatgc
     6181 ccccgaacaa agacaggaac atctgcgccc agcgtcagcc ccatttccgc cagctcatcc
     6241 atgcttagcc cgcattgcca gagatgattt aatgccacca ggaccgtcgc ggcattggat
     6301 gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt caatgctgat attcgcaccg
     6361 cttcccgtcg gaagacgccc gctgtctgcc gcagttttca tcaacaaccg cgctgcgcga
     6421 acgatcaggt tatcttcatg ttccacgcct tcaacgggcg ttaacagacg gatatcccca
     6481 tcgtcacgaa gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc
     6541 agcgtgtggt aaccatccgc acgctgaccg gtaatgtata aaaacagatt aagttttgcc
     6601 ggagagggcc actgtgtccg cattatttca ctatccagtt atccattttt aacttgatgc
     6661 gttgaccacc gtcggtgagt tccatattgg ctggcatcgc aggttgcgtt ttggtgtcat
     6721 aaccaccata aacaaccttc cagtttttgc cattctggct gtaggtaatt tcgctcaggc
     6781 ggtactggtc gtccagtttg tagtcggttg catcacccgg taaacctaaa atccactggc
     6841 gcaagctgtt gagcggaatt ggcattccag tcaatttgcc aatcatctct tcggcgtcat
     6901 cggcggtata acgctgacct ttattgtcga ctaactgcac gttacccggt tgagcattca
     6961 gctccagttc cgtgctgccc aatgggttag tgagcagcag acggtagcga tcctggccgg
     7021 tttgctgcca gaaaaagcgg gcgtacactt tttgttggtc agaaatataa gcgaacgcgc
     7081 cgcgagtctg atactgatta agattgcgca cgtcttgctg atgctgacgc cattgtggcg
     7141 aatccgggct tttgccagga cctttgggcg tggtaacgga acaggcagtg agcacaagag
     7201 cagccagcgg tagcaggcgg ataagacgaa aatcgggcag gggcatagtg atgacaagtc
     7261 cttgagatac gttgcagtta taacccttaa tgctagcgtc accattagtc atcgtctatg
     7321 ttcaagttgt cttaattgta gaaatctaac gggttcgggc aattactcca aaagggggcg
     7381 ctccctttta ttgatctcgc gcatacggta tgatgcgccg agactaacct tatcaacgtt
     7441 ggtattattt cccgcagaca tgaccctttt agcgctcggt attaaccata aaacggcacc
     7501 tgtatcgctg cgagaacgtg tatcgttttc gccggacaaa cttgatcagg cgctggacag
     7561 tctgctcgca cagccgatgg tgcagggcgg ggtggtgctg tcgacgtgta accgcactga
     7621 gctttatctt agcgtcgaag agcaggacaa cttgcaagag gcgttaatcc gctggctttg
     7681 cgattatcac aatcttaatg aagaagatct gcgtaaaagc ctctactggc atcaggataa
     7741 cgacgcggtt agccatttaa tgcgtgttgc cagcggcctg gattcactgg ttcttgggga
     7801 gccgcagatc ctcggtcagg ttaaaaaagc gtttgccgat tcgcaaaaag gtcatatgaa
     7861 ggccagcgaa ctggaacgca tgttccagaa atctttctct gtagcgaaac gcgtacgcac
     7921 tgaaacagat atcggtgcca gcgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg
     7981 gcagatcttt gaatcgctct ctacggtcac agtgttgctg gtaggcgcgg gcgaaaccat
     8041 cgagctggta gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta tcgccaaccg
     8101 cactcgcgaa cgtgcccaaa ttctggcaga tgaagtcggc gcggaagtga ttgccctgag
     8161 tgatatcgac gaacgtctgc gcgaagccga tatcatcatc agttccaccg ccagcccgtt
     8221 accgattatc gggaaaggca tggtggagcg cgcattaaaa agccgtcgca accaaccaat
     8281 gctgttggtg gatattgccg ttccgcgcga tgttgagccg gaagttggca aactggcgaa
     8341 tgcttatctt tatagcgttg atgatctgca aagcatcatt tcgcacaacc tggcgcagcg
     8401 taaagccgca gcggttgagg cggaaactat tgtcgctcag gaaaccagcg aatttatggc
     8461 gtggctgcga gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc aggcagagca
     8521 agttcgcgat gagttaaccg ccaaagcgtt agcggccctt gagcagggcg gcgacgcgca
     8581 agccattatg caggatctgg catggaaact gactaaccgc ttgatccatg cgccaacgaa
     8641 atcacttcaa caggccgccc gtgacgggga taacgaacgc ctgaatattc tgcgcgacag
     8701 cctcgggctg gagtagcagt acatcatttt ctttttttac agggtgcatt tacgcctatg
     8761 aagccttcta tcgttgccaa actggaagcc ctgcatgaac gccatgaaga agttcaggcg
     8821 ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt atcacgcgaa
     8881 tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat
     8941 atcgaaaccg cacagatgat gctcgatgat cctgaaatgc gtgagatggc gcaggatgaa
     9001 ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt tctgttactg
     9061 ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag tccgagccgg aaccggcggc
     9121 gacgaagcag cgctgttcgc aggcgatctg ttccgtatgt acagccgtta cgccgaagcc
     9181 cgccgctggc gggtagaaat catgagcgcc agcgagggtg aacatggtgg ttataaagag
     9241 atcatcgcca aaattagcgg tgatggtgtg tatggtcgtc tgaaatttga atctggcggt
     9301 catcgcgtgc agcgtgttcc tgctacggaa tcgcagggtc gtattcatac ttctgcttgt
     9361 accgttgcgg taatgccaga actgcctgac gcagaactgc cggacatcaa cccagcagat
     9421 ttacgcattg atactttccg ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat
     9481 tcggcaattc gtattactca cttgccgacc gggattgttg ttgaatgtca ggacgaacgt
     9541 tcacaacata aaaacaaagc taaagcactt tctgtactcg gtgctcgcat ccacgctgct
     9601 gaaatggcaa aacggcaaca ggccgaagcg tctacccgtc gtaacctgct ggggagtggc
     9661 gatcgcagcg accgtaaccg tacttacaac ttcccgcagg ggcgcgttac cgatcaccgc
     9721 atcaacctga cgctctaccg cctggatgaa gtgatggaag gtaagctgga tatgctgatt
     9781 gaaccgatta tccaggaaca tcaggccgac caactggcgg cgttgtccga gcaggaataa
     9841 tggaatatca acactggtta cgtgaagcaa taagccaact tcaggcgagc gaaagcccgc
     9901 ggcgtgatgc tgaaatcctg ctggagcatg ttaccggcag agggcgtact tttattctcg
     9961 cctttggtga aacgcagctg actgacgaac aatgtcagca acttgatgcg ctactgacac
    10021 gtcgtcgcga tggtgaaccc attgctcatt taaccggggt gcgagaattc tggtcgttgc
    10081 cgttatttgt ttcgccagcg accttaattc cgcgcccgga tacggagtgt ctggtggagc
    10141 aggcactggc gcggttgcct gaacaacctt gccgtattct cgatctcggg acgggtaccg
    10201 gggcgattgc gcttgcgctg gctcgcgagc gcccggactg cgaaattacc gctgtagatc
    10261 gtatgcccga tgctgtcgcc ctggcacaac gtaatgccca gcatctggcg atcaaaaata
    10321 tccacattct gcaaagcgac tggtttagcg cgctagccgg gcagcagttt gcgatgattg
    10381 tcagcaatcc gccgtatatt gacgagcagg accctcatct tcaacaaggc gatgtccgct
    10441 ttgagccgct cactgcgctg gttgcggcag acagtggaat ggcagacatc gtgcatatca
    10501 tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct gcttctggaa catggctggc
    10561 agcagggcga agcggtgcga caggcattta tcctcgcggg gtatcatgac gtcgaaacct
    10621 gccgtgacta tggtgataac gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt
    10681 ttctacactg cttagtgttc atcttattag tatcgcgctt tctgttgggc tattaacctt
    10741 acgtttctgg ctacgttatc agaagcatcc acaggcattt gctcgctgga cgcgcattgt
    10801 gccgccggtt gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca
    10861 catcctgcca ttttccgggc aggcacagtg gctgactgaa aagctgtttg gagttatcat
    10921 ttatatcgtt ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc agcaggcgcg
    10981 cattattgcc ttcccgctga cgttggtggt gctgtacatc atcattaaac tcgccaccac
    11041 aaaagtaccg ttactggggt aagtcatgag atcgttagct gatttcgaat ttaataaagc
    11101 gccattgtgc gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca
    11161 agatgtttac gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa tcagccagct
    11221 tctgccttta gaagagcagc tggaaaaact gatcgcgctg ttttacggcg actggggatt
    11281 taaagcctca cgcggcgttt atcgtctttc cgatgcatta tggctggacc aggtgttaaa
    11341 gaatcgacag ggcagtgcgg tatcattagg tgcggtttta ttatgggtcg cgaatcgtct
    11401 cgatttgccg ctgctgccgg tgattttccc tacgcagctg atattgcgca ttgaatgtcc
    11461 ggatggcgaa atttggctga ttaatccttt taacggtgaa tcgttaagcg aacatatgct
    11521 ggacgtatgg ttaaagggaa atatcagccc gtcggcggaa ctgttttatg aagaccttga
    11581 tgaagctgat aacattgagg taatccgcaa attgctggat acactcaaag cctcgttgat
    11641 ggaagaaaat cagatggagc tggcgttacg caccagcgaa gctttattac aattcaaccc
    11701 ggaagatccc tatgaaattc gcgatcgcgg gttgatttat gcgcaactgg attgcgaaca
    11761 cgttgcgttg aacgatttaa gttatttcgt tgaacagtgt ccggaagacc cgatcagcga
    11821 aatgatccgt gcgcaaataa ataacatcgc gcataaacat attgtgctgc attaattaat
    11881 cgacatttta ctcaaggtta aggcgatcct atgaaacaaa aagtggttag cattggcgac
    11941 atcaacgtag caaatgacct gccgttcgta ctgtttggcg gtatgaacgt gctggaatct
    12001 cgcgatctgg cgatgcgcat ttgcgagcac tacgtaaccg tgacccagaa actgggtatc
    12061 ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct cctccatcca ctcttatcgt
    12121 ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt tgaagcagac ttttggcgtg
    12181 aaaattatca ccgacgttca cgaaccaagt caggcacagc ccgttgctga tgtcgtggat
    12241 gtgattcagt tgccggcgtt tcttgctcgc cagactgacc tggttgaagc catggcgaaa
    12301 accggtgcgg taattaacgt caagaaacca cagtttgtca gcccgggaca gatgggtaat
    12361 atcgttgata aattcaaaga aggcggcaac gaaaaagtga ttctttgcga tcgtggtgct
    12421 aacttcggct atgacaacct ggttgtcgat atgctgggct tcagcattat gaagaaagtg
    12481 tctggtaact cgccggtgat tttcgacgtg acccacgcat tgcaatgccg cgatcctttt
    12541 ggcgcagctt ccggcggtcg tcgtgctcag gtggctgagc tggcacgagc cggtatggcg
    12601 gtaggtctgg cggggctgtt tattgaagcg catccggatc cggaacatgc gaaatgtgat
    12661 ggtccatccg cgctgccgct ggctaaactg gaaccgttcc tcaggcagat gaaagcgatt
    12721 gatgatctgg tgaaaggttt cgaagaactg gataccagca agtaatcttt tttgcttgaa
    12781 aaataaagtc ttagcgttct gcgttaagac ttttttcatg ggtgccggat acaaaaaagg
    12841 ccgcaggctg ttaccctgcg gccggtttcg ggcgcatatt gccatcacgg cagcctgacg
    12901 cccgttttcg ccttacttcc ggttacgcca ccagccgaca atcgctgcgg taataattcc
    12961 cgccaggatc ggtgctgcca ggtcgtgcca gaaaatcatg gcaaactgcg cgagcgtcat
    13021 atagccgcct tgttgtaatg acaacatttt gcggctattc ttgaattgtt gatggttcaa
    13081 gattagcccc cgttctgttg tcaggtttta cctctcaacg tgcgggggtt ttctctttcc
    13141 agcaaccaat gccaccaggg ataaagcccc cgcaacattg cgcctcaccg gataacgccg
    13201 gcttggtgtg gatactacga ctcaattcat cttcacttca tccctgaaat gtttgcaatg
    13261 aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagtgc tctggatgat
    13321 gcttccagct cgggttgcca atatttactt gtggaagtga taaagacaaa aatggccgca
    13381 ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc ctgacgcccg
    13441 ttttcacctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat aattcccgcc
    13501 aggatcggtg ccgccaggtc gtgccagaaa gtcatggcaa actgcgcgag cgtcatatag
    13561 ccgccttgtt gtaatgacaa cattttgcgg ctattcttga agtgtctggt ttcaagatta
    13621 gccctgttgt caggttttac ctctcaacgt gcgggggttt tctctttcca gcaaccaatg
    13681 ccaccaggga taaaaccccc gcaacattgc gcctcaccgg ataacgccgg cttggtgtgg
    13741 atactacgtc tcaattcatc ttcacttcat ccctgaaatg tttgcaataa agagtacatt
    13801 ccggcttttc aacagctgtt gcagtggttt cgtgcaggtt gtggtacagg ctcgcaattc
    13861 tgattaacga cctgtccagg tagtatgaag aaatacaata agcaggaatt tatattatct
    13921 cccccggttt ccgtcatcgg aggagatata actgtcaggc aaatatcgtc atcaaatagg
    13981 cggcaaacag tgccagatgc gctgcgccat tgagcacgtt agtacgtccg gtggagaagg
    14041 agatatggca cagcactaaa gaggccacca tcaccaccat ttctggcgca ccaagtgcaa
    14101 actgcaattc gttacccgtc ataaaggcaa ttagcgtgac gacaggtacg gtaagcgaaa
    14161 ttgttgctaa cactgaacca aagaacagat tcatcgcgcg ctggacctgg ttgttcaaca
    14221 ctgcttttaa tgcacctaaa ccttccggcg acagaatcaa cagtgccacc aggaagccag
    14281 taaaggcgac aggggcattc atgctgtcga gcaaagtctc cagcgggctg gcgttcattt
    14341 tggtgactgc aataacggca atcaggtgga taatcaacca gatagcatgc cacaggctgc
    14401 tatgggcaga cggtttaccg tggtgcgggt cgtcgtcatc actgtcatct tcgtgctcgt
    14461 agacaaacaa actttgatgc gttttggtct ggatcagcaa aaatacacca tacattgccg
    14521 cagaaattaa tgctaccagt aacgcctgac cggttgaaaa attcgccgca ggcagagcca
    14581 ttggaaatac cagtacgatt atcgccaggg ggaacagcgc aattaaatac tgcttaatac
    14641 caaacagatt catatattgg gtggcaaact tacgaccgcc caacaataat gaaaagccga
    14701 ccagcccacc ggtaacaatc ataatgatcg aatagagcgt atcacgcatt agcgttggcg
    14761 cggcgtcgcc ggttgccatt aaagctgaaa tcaaactaac ttcaagaata accactgaaa
    14821 ggctaagaat aagcgaaccg taaggttctc ccaggcgatg ggctaatacg tccgcatgac
    14881 ggacaacact aaaggcgcta cttaaaatac caataagcgc aagaagattg atggcaatga
    14941 ccactggtag tgtctggctg cttccccaca ggaacagcac taccagcgcc agaaccggga
    15001 aaataagcga agtctccttg tggcgggttt ttaccgcctc ttgagcattt gacattatgg
    15061 ttatcccttt gc
//
LOCUS       DAZFKY010000115        14980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFKY010000115 DAZFKY010000000
VERSION     DAZFKY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHKCL_003152"
     CDS             complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHKCL_003152"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5764066.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1321..1734
                     /gene="holD"
                     /locus_tag="ACHKCL_003153"
     CDS             1321..1734
                     /gene="holD"
                     /locus_tag="ACHKCL_003153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5764067.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1703..2149
                     /gene="rimI"
                     /locus_tag="ACHKCL_003154"
     CDS             1703..2149
                     /gene="rimI"
                     /locus_tag="ACHKCL_003154"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5764068.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHKCL_003155"
     CDS             2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHKCL_003155"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5764069.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2932..4521
                     /gene="prfC"
                     /locus_tag="ACHKCL_003156"
     CDS             2932..4521
                     /gene="prfC"
                     /locus_tag="ACHKCL_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5764070.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4914..5519
                     /gene="osmY"
                     /locus_tag="ACHKCL_003157"
     CDS             4914..5519
                     /gene="osmY"
                     /locus_tag="ACHKCL_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5764071.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5646..5807
                     /locus_tag="ACHKCL_003158"
     CDS             5646..5807
                     /locus_tag="ACHKCL_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5764072.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5929..7002
                     /locus_tag="ACHKCL_003159"
     CDS             5929..7002
                     /locus_tag="ACHKCL_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5764073.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            6999..7781
                     /locus_tag="ACHKCL_003160"
     CDS             6999..7781
                     /locus_tag="ACHKCL_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5764074.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(7995..8858)
                     /locus_tag="ACHKCL_003161"
     CDS             complement(7995..8858)
                     /locus_tag="ACHKCL_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5764075.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(8830..10379)
                     /locus_tag="ACHKCL_003162"
                     /pseudo
     CDS             complement(8830..10379)
                     /locus_tag="ACHKCL_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            10637..11416
                     /gene="deoC"
                     /locus_tag="ACHKCL_003163"
     CDS             10637..11416
                     /gene="deoC"
                     /locus_tag="ACHKCL_003163"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5764076.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11543..12865
                     /gene="deoA"
                     /locus_tag="ACHKCL_003164"
     CDS             11543..12865
                     /gene="deoA"
                     /locus_tag="ACHKCL_003164"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5764077.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12917..14140
                     /gene="deoB"
                     /locus_tag="ACHKCL_003165"
     CDS             12917..14140
                     /gene="deoB"
                     /locus_tag="ACHKCL_003165"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5764078.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14197..14916
                     /gene="deoD"
                     /locus_tag="ACHKCL_003166"
     CDS             14197..14916
                     /gene="deoD"
                     /locus_tag="ACHKCL_003166"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5764079.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3548 a         3917 c         4064 g         3451 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat caggagacta
      121 acgtgcaggt tttgtaagtc ggataaggcg ttcacgccgc atccgacacg gtattcggcg
      181 agataattaa cctttcttcg cctggcgggt cataatggcg cgatacacct tgaagcgccc
      241 tgtttgcgcg atcacttcgt ggaagccaaa tgtctcatcc agcacgtccg gataaggcag
      301 gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca ccgcaccgcg
      361 aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga
      421 gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc
      481 ttcaacaccg ttggccgcaa gtgttgcgcg gctggcttct accgctggcg cagagacatc
      541 gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag gcaaccgaaa gcacgcccgc
      601 gccacagccg acatccagca ctttaccttt cgtgtgcggg gttaacgtcg agagcagcaa
      661 ctggctaccg acatccagac cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag
      721 gccatcgacg ctgtattcgc cccagaattt atcggcatca aataccggct gtttttccag
      781 acgaccaaaa tagaggccac agcgacgggc gctgtcgact ttattcaacg gcgcataatc
      841 agccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacaa caaaaatatc
      901 cgtccccacc ggaagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt
      961 cttcggccag tagtaaatca gcgtatcgca atctgcgaca tcatccgccg tggcgaccag
     1021 actaaaacgg gcgttatccc ccatctggcg gcttaacacc tgccaatgct ggaattgctg
     1081 ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc
     1141 ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc
     1201 cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat
     1261 gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct
     1321 atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt
     1381 cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cggcacacgt ccgtctggtg
     1441 atggtggcaa acgatcttcc cgccctgact gatcctttag tgagcgatgt tctgcgcgca
     1501 ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg
     1561 caaggcagtc gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc
     1621 gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg
     1681 ttatggcaac aaatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc
     1741 agcggcttac cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc
     1801 cagcaaccag ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc
     1861 gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg tggtcgatcc
     1921 tgactatcag cgtcagggat tgggaagggc gctgctggaa catctgatcg acgaactgga
     1981 aaaacgcggc gtggcgacac tatggctgga agtccgtgcc tcaaatgctg ccgccattgc
     2041 cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact accccaccac
     2101 ggacggtcgc gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga
     2161 ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca
     2221 ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt
     2281 caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact
     2341 tcattacagc ttcagcacgg gcggtttgag agctgggccg aacggctgaa cgtcgagcca
     2401 ggtaaactca acgaagcctt tattaatgcg atggcggaaa tctgcacgcc gctgccgggc
     2461 gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc
     2521 tttagtgcct tgcaacaggt gcgtctggaa cgcacgggcc tgcgtgatta cttcgatttg
     2581 ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg
     2641 ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga cactgccgag
     2701 tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcacaccat
     2761 cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag
     2821 cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt
     2881 ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg
     2941 tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcacccggac
     3001 gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc
     3061 ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat ggagatggaa
     3121 aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca cgattgcctg
     3181 gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta tcgtaccctg
     3241 acggcggtgg actgctgcct gatggttatc gacgccgcaa aaggtgttga agatcgtacc
     3301 cgtaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa
     3361 cttgaccgtg atatccgcga cccgatggaa ttgctcgatg aagttgagaa cgagctgaaa
     3421 attggctgcg caccgatcac ctggccaatt ggctgcggca agctgtttaa aggcgtttac
     3481 cacctttata aagatgaaac ctatctctat cagagcggta aagggcacac cattcaggaa
     3541 gtccgcattg ttaaagggct gaataacccg gatctcgatg ctgcggttgg tgaagatctg
     3601 gcacagcagc tgcgtgacga actggaactg gtgaaaggcg cgtctaacga gttcgacaaa
     3661 gagctgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct gggtaacttc
     3721 ggcgttgatc atatgctgga tggcctggtg gagtgggcgc ctgcgccgat gccgcgtcag
     3781 accgataccc gtaccgtaga agcgagcgaa gataaattta ccggcttcgt atttaaaatt
     3841 caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgtgt ggtgtccggt
     3901 aaatatgaaa aaggcatgaa gctgcgccag gtgcgcactg cgaaagatgt ggtgatctcc
     3961 gacgcgctga cctttatggc gggtgaccgt tcgcacgttg aagaagcgta tccgggcgat
     4021 atcctcggcc tgcacaacca cggcaccatt cagatcggcg acacctttac ccagggtgag
     4081 atgatgaagt tcaccggtat tccgaacttc gcaccagaac tgttccgtcg tatccgcctg
     4141 aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc cgaagagggc
     4201 gcggtgcagg tgttccgtcc aatctccaac aacgatctga tcgttggtgc ggttggtgtg
     4261 ctgcagtttg atgtggtggt agcgcgcctg aagagcgaat acaacgttga agcagtgtat
     4321 gagtcagtca acgttgccac tgcccgctgg gtagaatgtg cagacgcgaa gaaattcgaa
     4381 gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa cctcgcttac
     4441 atcgctacca gcatggtcaa cctgcgcctg gcacaggaac gttatccgga cgttcagttc
     4501 caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg cgtcctgtca
     4561 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt
     4621 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaaa gtgatgacat
     4681 ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc cgtcgattgt
     4741 cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc atcactcatc
     4801 gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct attcagcaat
     4861 gcaacctcgt gttgccaggc tcaaattacg agcaaacata caggaataaa tcgatgacta
     4921 tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct gccgtcgcga
     4981 ccggctctgc ctacgcggaa aacaacgcgc agactaccaa tgaaagcgca gggcaaaaag
     5041 tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc accgcgaaag
     5101 tgaaggcggc gctggtggat catgacaaca tcaagagcac cgatatctct gtcaaaaccg
     5161 atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggcccaggcc gaagaggcag
     5221 tgaaagtggc gaaaggtgtt gaaggggtga cctctgtcag cgacaaactg cacgttcgcg
     5281 acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc agtgaaatca
     5341 aagccaaatt gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg
     5401 atggcgtggt tcagctctct ggtatcgtag attctcaggc acaaagtgac cgtgctgaaa
     5461 gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat
     5521 tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca cactaaaaat
     5581 atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg gtaaaggaga
     5641 cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc gccgcacttg
     5701 ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt gtcgtcggga
     5761 ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt tcagaccatt
     5821 aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta gcgaataata
     5881 aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt ggggcagcga
     5941 atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca gcctggacga
     6001 atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg cgtgctggat
     6061 gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc tgccggggcg
     6121 cagaacctct cggcgtatat ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc
     6181 tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg aaatcttatc
     6241 gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat ggataccgcc
     6301 gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca ggatgactac
     6361 gcgccgaatt actttttacc aaccaaacaa aactggctgg atgtgattcg cgcctccagt
     6421 gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta cctggatggc
     6481 gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa aacgttggtc
     6541 gtcatccgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa acgcatggaa
     6601 cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc
     6661 agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg gatattcgaa
     6721 atttatccgc cgaagccatt acatagtatc gcgcttggca gtcggattcc ggcgctgcgt
     6781 gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt tggcaagcta
     6841 ttaactgaaa aagcgccgct tacccgccat ctggtgccag tggtgacgcc ggaatcgatt
     6901 gtcattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag cgacgctccg
     6961 caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt tttatcgaca
     7021 cccactgcca ttttgatttc ccgcctttta ttggcgatga agaggccagc ctgcaacgcg
     7081 cggcacaagc gggcgtaggc aagatcattg ttccggcaac cgaggcggaa aattttgccc
     7141 gcgtgcaggc gttagcggaa aattatcaac cgctgtatgc cgcattgggc ttgcatcctg
     7201 gtatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc
     7261 gtccggcgaa ggtggtggcg gtgggggaga gcggtctgga tctcttcggc gacgatccgc
     7321 aatttgagag gcagcagtgg tttctcgacg aacaactgaa actggcgaaa cgctacgatc
     7381 tgccggtgat cctgcattca cggcgcacac acgacaaact ggcgatgcat cttaaacgcc
     7441 acgatttatc gcgcactggc gtggttcacg gtttttccgg cagcctgcaa caggccgagc
     7501 ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat ccacgcgcca
     7561 gtaaaacccg cgttgtcatc gcaaaattac cgctggcatc gttgttgctg gaaaccgacg
     7621 cgccggatat gccgctcaac ggttttcagg ggcagcctaa ccgcccggaa caggctgcgc
     7681 gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt gccgaagtgt
     7741 tgcttaataa cacgtatgcg gtgtttaacg ttcgtgggta gtttgccctt cgtaggcagc
     7801 atccggcagt cggcgcataa tgcctgatgc gacgcttaac gcgtcttatc atgcctacag
     7861 gtttgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca
     7921 atgcctgatg cgacgcttga cgcgtcttat caggcctaca agtctgtgcc gaaccgtagg
     7981 tcgtatccgg catgtcacaa atacagcgcc ggaaatatta accggctcac cccgcgctcc
     8041 cgtaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc
     8101 tcgccataca cgccgtgggc atgaaacgcg ttcaggcgta ccggaacatc gccgactccc
     8161 ttgataaacg ccgccagttc ttcgatgtgt tgcaaataat ccacttggcc agggattacc
     8221 agcaaacgca gttccgccag cttaccgcgc tctgccagca aacagatgct gcgcttaatc
     8281 tgctgattat cgcgtccggt taattgctga tgacattcgc tcccccacgc tttgagatcg
     8341 agcattgcgc cgtcgcacac cgggagcaat ttttcccagc cggtttcgct caacataccg
     8401 ttactgtcca ccaggcaggt gaggtggcgc agttgcggat cgtttttgat agcagtaaac
     8461 agcgccacta caaacggcag ttgggtcgtg gcttcgccgc cactcaccgt tatcccttcg
     8521 ataaacagca ctgctttgcg gacatggcta agcacctcat ccacgctcat ggattgcgcc
     8581 attggcgtgg catgttgcgg acacctcttc aggcaggtat cacattgctc gcagaccaca
     8641 gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg
     8701 cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt tttgcagcgc
     8761 agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac gcaggagaag
     8821 gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc gcggctggcg
     8881 ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga
     8941 accttcggcg cgatattttt ctaaatccga caaacgcacc atataaccgg taacgcgaac
     9001 cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt ccggctttaa aggcaccgag
     9061 gcaaagctgt accaacgcct gcgggttacg tttgatggtt tcgtcgagcg tcagaatgtc
     9121 gctgatgcca gaataataat aagcatgatg cgggcgacag tttgcagatg ggtgatcgga
     9181 tctggctcat cgccatacgg caaacgcgcg cccggcgtgg tgccgatatc ggaactgatc
     9241 cccgactgtg cgtgtaacat ggcgcgtttt tgccagccat atttcacggg ggtattggcg
     9301 acaaactccg ccagctgtgc actgatgcga taacctactt catttgcggc ggcttcttta
     9361 ccgtagcgcg cggcaatccc ttctttttca cacagcaagt taaccgcttc cgccagccca
     9421 tacatgccaa acattggcac aaaacgttca gggttaatca gcccttcttt caccaggaag
     9481 ctattctcaa agaagtgtga ttgttgatag aggaattcac accgcgcatc gatgatggcg
     9541 atctgctgct ggcagtagtg cggtagagtg cgcgtaaaga agtcatccag cgattcgctg
     9601 cgctcggcaa tggctttcag gttaaggcgt accagcgtgc tgccaccacc cgccagcggc
     9661 agtgagttgt aacagctcac aatcccgtag cccccttttg tgaaaatttt atcatgcacc
     9721 ggaccgttgg cgatgtgcgg tttgctacat tcacagatgt tcttcgccac ttccagcagc
     9781 aggtcatcag gggtgatttc aggatcgtag ataaaggtca ggttcggtga aacctgcttc
     9841 aaatctgcgt ctgcacgtaa gatcgcgcgg gtaatgggcg aatcagacgg gccgatattg
     9901 gcgtgcataa aggcgtctgg cagggttctg tcgaggtaac gccagaaacg ttttattcga
     9961 acatcgatct cgtcttgtgt tagaattata acatacggtt gcaacaacgc atccagttgc
    10021 cccaggtaga ccggcatcga tgtgaccgac ggtacgtggt ggtaaagaat ggtcagcaga
    10081 gagagtgcgt catcaagatc tttcgcgcct tccagctcca gccattcgga accgttcgcc
    10141 agaaaacggg cgtaatcggg taagacatag cgcggtttgt acggcgcatg accttcaaac
    10201 atatcgcaga ttacaccttc atccagcgcg cggcgggctt cggcaggaag ctgtgggtaa
    10261 ggcagattgt tttctgcttc cagtgccaga aaatggcgct tctgctccgg gctaagcact
    10321 gggctggtga caatttgctg gcaacgttgt tgcagtgcat tttcatgaga agtgggcatc
    10381 ttcttttcct tttatgccga aggtgatgcg ccattgtaag aagtttcgtg atgttcactt
    10441 tgatcctgat gcgtttgcca ccactgacgc attcatttga aagtgaatta tttgaaccag
    10501 atcgcattat agtgatgcaa acttgtaagt agatttcctt aattgtgatg tatatcgaag
    10561 tgtgttgcgg agtagatgtt agaatactaa caaactcgca aggtgaattt tattggcgac
    10621 aagccaggag aatgaaatga ctgatctgaa agcaagcagc ctgcgtgcac tgaaattgat
    10681 ggacctgacc accctgaatg acgacgacac cgacgagaaa gtaattgctc tgtgtcatca
    10741 ggccaaaact ccggtcggca ataccgccgc tatctgtatc tatcctcgct ttatcccgat
    10801 tgctcgcaaa acactgaaag agcagggcac cccggaaatc cgtattgcta cggtaaccaa
    10861 cttcccacac ggtaacgacg acatcgaaat cgcgctggca gaaacccgtg cggcaatcgc
    10921 ctacggtgct gatgaagttg acgttgtgtt cccgtaccgc gcgctgatgg cgggtaacga
    10981 gcaggttggt tttgacctgg tgaaagcctg taaagaggct tgcgcggcag cgaatgtact
    11041 gctgaaagtg atcatcgaaa ccggcgaact gaaagacgaa gcgctgatcc gtaaagcgtc
    11101 tgaaatctcc atcaaagcgg gtgcggactt catcaaaacc tctaccggta aagtggctgt
    11161 gaacgcgacg ccggaaagcg cgcgcatcat gatggaagtg atccgtgata tgggcgtaga
    11221 aaaaaccgtt ggtttcaaac cggcgggcgg cgtgcgtact gcggaagatg cgcagaaata
    11281 tctcgccatc gcagatgaac tgttcggtgc tgactgggct gatgcgcgtc actaccgctt
    11341 tggtgcttcc agcctgctgg caagcctgct gaaagcgctg ggtcacggcg acggtaagag
    11401 cgccagcagc tactaagtaa gatgctttac gcctgatgcg ctgcgcttat caggcctacg
    11461 agacgtatct acccgtaggc cggataaggc gtagacgcat ccggcaaaag ccgcctcata
    11521 ctcttttcct cgggaggtta ccttgtttct cgcacaagaa attattcgta aaaaacgtga
    11581 tggtcatgcg ctgagcgatg aagaaattcg tttctttatc aacggtattc gcgacaacac
    11641 tatctccgaa gggcagattg ccgccctcgc gatgaccatt ttcttccacg atatgacaat
    11701 gcctgagcgt gtctcgctga ccatggcgat gcgagattca ggaaccgttc tcgactggaa
    11761 aagcctgcat ctgaatggcc cgattgttga taaacactcc accggtggcg tcggcgatgt
    11821 gacttcgctg atgttggggc cgatggtcgc agcctgcggc ggctatattc cgatgatctc
    11881 tggtcgcggc ctcggtcata ctggcggtac gctcgacaaa ctggaatcca tccctggctt
    11941 cgacattttc ccggatgaca accgtttccg cgaaattatt aaagacgtcg gcgtggcgat
    12001 tatcggtcag accagctcac tggctccggc ggataaacgt ttctacgcga cccgtgatat
    12061 taccgcaacc gtggactcca tcccgctgat cactgcctcg atcctggcga agaaactggc
    12121 ggaaggtctg gacgcgctgg tgatggacgt gaaagtgggt agcggcgcgt ttatgccgac
    12181 ctacgaactc tctgaagccc ttgccgaagc gattgttggc gtggctaacg gcgcaggcgt
    12241 gcgcaccacc gcactgctca ccgacatgaa tcaggtgctg gcctccagtg ccggtaacgc
    12301 ggttgaagta cgtgaagcgg tgcagttcct gacgggtgaa tatcgtaacc cgcgtctgtt
    12361 tgatgtcacg atggcgctgt gtgtggagat gcttatctcc ggcaaactgg cgaaagatga
    12421 cgccgaagcg cgcgcgaaat tgcaggcggt gctggacaac ggtaaagcgg cagaagtctt
    12481 tggtcgtatg gtagcggcac aaaaaggccc gaccgacttc gttgagaact acgcgaagta
    12541 tctgccgaca gcgatgctga cgaaagcagt ctatgctgat accgaaggtt ttgtcagtga
    12601 aatggatacc cgcgcgctgg ggatggcagt ggttgcaatg ggcggcggac gccgtcaggc
    12661 atctgacacc atcgattaca gcgtcggctt tactgatatg gcacgtctgg gcgaccaggt
    12721 agacggccag cgtccgctgg cggttatcca cgcgaaagac gaaaacagct ggcaggaagc
    12781 ggcgaaagcg gtgaaagcgg caattaaact tgccgataaa gcaccggaaa gcacaccaac
    12841 tgtctatcgc cgtatcagcg aataacggta tactgatctg atcatttaaa tttgaagcac
    12901 tgagtacgga gaacatatga aacgtgcatt tattatggtg ctggactcat tcggcatcgg
    12961 cgctacagaa gatgcagaac gctttggtga cgtcggggct gacaccctgg gtcatatcgc
    13021 agaagcttgt gccaaaggcg aagctgataa cggtcgtaaa ggcccgctca atctgccaaa
    13081 tctgacccgt ctggggctgg cgaaagcaca cgaaggttct accggtttca ttccggcggg
    13141 aatggacggc aacgctgaag ttatcggcgc gtacgcatgg gcgcacgaaa tgtcatccgg
    13201 taaagatacc ccgtctggtc actgggaaat tgccggtgtc ccggttctgt ttgagtgggg
    13261 atatttctcc gatcacgaaa acagcttccc gcaagagctg ctggataaac tggtcgaacg
    13321 cgctaatctg ccgggttacc tcggtaactg ccactcttcc ggtacggtca ttctggatca
    13381 actgggcgaa gagcacatga aaaccggcaa gccgattttc tatacctccg ctgactccgt
    13441 gttccagatt gcctgccatg aagaaacttt cggtctggat aaactctacg aactgtgcga
    13501 aatcgcccgt gaagagctga ccaacggcgg ctacaatatc ggtcgtgtta tcgctcgtcc
    13561 gtttatcggc gacaaagccg gtaacttcca gcgtaccggt aaccgtcacg acctggctgt
    13621 tgagccgcca gcaccgaccg tgctgcagaa actggttgat gaaaaacacg gccaggtggt
    13681 ttcggtcggt aaaattgcag acatctacgc caactgcggt atcaccaaaa aagtgaaagc
    13741 gactggcctg gacgcgctgt ttgacgccac catcaaagag atgaaagaag cgggtgataa
    13801 caccatcgtc ttcaccaact tcgttgactt cgactcttcc tggggccacc gtcgcgacgt
    13861 cgccggttat gctgcgggtc tggaactgtt cgaccgccgt ctgccggagc tgatgtctct
    13921 gctgcgcgat gacgacatcc tgatcctcac cgctgaccac ggctgtgatc cgacctggac
    13981 cggtactgac cacacgcgtg aacacattcc ggtactggtt tacggcccga aagtaaaacc
    14041 gggctcactg ggtcaccgtg aaaccttcgc ggatatcggc cagactctgg caaaatattt
    14101 tggtacttct gatatggaat atggcaaagc catgttctga tggatttggg cggagcgttg
    14161 actccgcctt tgttatgtca caaaaaggat aaaacaatgg ctaccccaca cattaatgca
    14221 gaaatgggcg atttcgctga cgtagttttg atgccaggcg acccgctgcg tgcgaagtat
    14281 attgctgaaa ctttccttga agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc
    14341 ttcaccggta cttacaaagg ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg
    14401 tcctgctcca tctacaccaa agaactgatc accgatttcg gcgtgaagaa aattatccgc
    14461 gtgggttcct gtggcgcagt tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg
    14521 ggtgcctgca ccgattccaa agttaaccgc atccgtttta aagaccatga ctttgccgct
    14581 atcgctgact tcgacatggt gcgtaacgca gtagatgcag ctaaagcact gggcattgat
    14641 gctcgcgttg gtaacctgtt ctccgctgac ctgttctact ctccggacgg cgaaatgttc
    14701 gacgtgatgg aaaaatacgg cattctcggc gtggaaatgg aagcggctgg tatctacggc
    14761 gtggctgcag agtttggcgc gaaagccctg accatctgca ccgtgtctga ccacatccgc
    14821 actcacgagc aaaccactgc cgctgagcgt cagaccacct tcaacgacat gatcaaaatc
    14881 gcactggaat ccgttctgct gggcgataaa gagtaattgt gtttcgctga aaggcgattg
    14941 tcgtgttaag ccggagtggg ggactgctcc ggctttttag
//
LOCUS       DAZFKY010000116        14957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFKY010000116 DAZFKY010000000
VERSION     DAZFKY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..347
                     /locus_tag="ACHKCL_003167"
     CDS             78..347
                     /locus_tag="ACHKCL_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5764080.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHKCL_003168"
     CDS             316..1434
                     /locus_tag="ACHKCL_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5764081.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHKCL_003169"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHKCL_003169"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5764082.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHKCL_003170"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHKCL_003170"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5764083.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHKCL_003171"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHKCL_003171"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5764084.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHKCL_003172"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHKCL_003172"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5764085.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHKCL_003173"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHKCL_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5764086.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHKCL_003174"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHKCL_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5764087.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHKCL_003175"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHKCL_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5764088.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHKCL_003176"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHKCL_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5764089.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHKCL_003177"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHKCL_003177"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5764090.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHKCL_003178"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHKCL_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5764091.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHKCL_003179"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHKCL_003179"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5764092.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3739 a         3881 c         3816 g         3521 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag atgtgatctg
    14941 gatcacatac aagatat
//
LOCUS       DAZFKY010000117        14854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFKY010000117 DAZFKY010000000
VERSION     DAZFKY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(54..335)
                     /locus_tag="ACHKCL_003180"
     CDS             complement(54..335)
                     /locus_tag="ACHKCL_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFO5764093.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(597..1706)
                     /gene="apbC"
                     /locus_tag="ACHKCL_003181"
     CDS             complement(597..1706)
                     /gene="apbC"
                     /locus_tag="ACHKCL_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5764094.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1838..3871
                     /gene="metG"
                     /locus_tag="ACHKCL_003182"
     CDS             1838..3871
                     /gene="metG"
                     /locus_tag="ACHKCL_003182"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5764095.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4012..4740
                     /locus_tag="ACHKCL_003183"
     CDS             4012..4740
                     /locus_tag="ACHKCL_003183"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFO5764096.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6697..7821
                     /locus_tag="ACHKCL_003184"
                     /pseudo
     CDS             6697..7821
                     /locus_tag="ACHKCL_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7834..10819
                     /locus_tag="ACHKCL_003185"
                     /pseudo
     CDS             7834..10819
                     /locus_tag="ACHKCL_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10829..14458
                     /gene="yehI"
                     /locus_tag="ACHKCL_003186"
     CDS             10829..14458
                     /gene="yehI"
                     /locus_tag="ACHKCL_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5764097.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14520..14837
                     /gene="yehK"
                     /locus_tag="ACHKCL_003187"
     CDS             14520..14837
                     /gene="yehK"
                     /locus_tag="ACHKCL_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5764098.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3966 a         3692 c         3615 g         3581 t
ORIGIN      
        1 attcatagga atgattttgg ctttttaaat atgcttaaaa cggtgttggc aaattaatcg
       61 tagcgattta acacgatttt gcgtttgtcg ttattaccca cagctaccac ttctgtcgta
      121 acgccttttg cagtagaaga agcacgatag ttggtttcaa ctcgttgtaa caactgccca
      181 caacgttgct gaagatgatt attaacaaca tccatactgc atgtagggga actaattctg
      241 ccattgatgg ttaatgttgt agtatcagaa gagaacgcgg gtgatgctag tccataagca
      301 agaaaaacag ctcctgacaa ccaatactta ttcattgaac gttatccctg tagtaaaggt
      361 tatgccgggt agattgagtg cataacacac tatagctgta catccgctac acagccacga
      421 gggatgataa agagacagtg gttgcacaat caatcgcctt tgtacagttt cggactaaga
      481 tccactgaat attcgcagta ttaaataagt gttcattaat gcggcaaaaa caaatacacc
      541 gcatcaatat aacatctctg cggtgtatga agaataacca gaatggttaa taggcgttag
      601 accgcgcgga aggaaatctc gcctggaatg acttcaccct gccagtagag ctgagctgca
      661 acgcggtcag ccagctggcg gtagatagcg gtaaattcgc tctccggacg gctgataacc
      721 gttggcgtgc ctttatcgag atcctcacgt aaggagatgt gtaacggcat ctgccccagc
      781 agctgtgtat ggtatttttc agccagtttc tctgcaccac cggtaccgaa aatcggttca
      841 tggtgaccac agttactgca aatatgcaca ctcatgttct cgacaatacc gagcaccgga
      901 acctcgactt tctcgaacat cacaatgcct tttttcgcat cgatcaacgc gatgtcctgc
      961 ggggtggtga ccacgactgc accagttact ggaatgtttt gcgccagcgt caattgaatg
     1021 tcaccggtgc ctggcggcat atcaagcacc agatagtcga gatccggcca caaggtttcc
     1081 tgcaacatct gcattaacgc cttgctcgcc atcggtccac gccacaccat cgcattgtcg
     1141 tcagtgacca gataaccaat tgaattggtt gccaggccat gagacataat cggtgccata
     1201 tgggtaccgt ccggcgaagt tggacgctga ttttctgcgc ccagcatggt gggaattgat
     1261 gggccgtaga tatcggcgtc cagaatgccg actttcgccc cttccgccgc cagtgccagc
     1321 gccaggttta ccgccgtgga ggatttaccc acgccgcctt taccggaact gaccgcaatg
     1381 atgttcttca cgccattaat acccggctga ttcttcacgc gttttaaggt ggcgatattg
     1441 tgcgacagtt tccagtcgat agctttcgcc ccggtgatac gcagcagttc ggcgctacat
     1501 tgctctttta actcttcaaa cgcgctatgc cagacgaatg gcataaccag ttccacatgc
     1561 aacgtgtcgt ccatccaggc gacatggtgc aacgctttca gcgtggtgag gttatgcttc
     1621 agggttgggt gctgaaaatt ggccagtgtc ccggcgacca ttgcgcgcaa ggcttctggt
     1681 gatttggcct gggattgttc gttcatcccg actccttttt tgtatagata aaccatcagc
     1741 tgatagttta cctgaagaat atagagaagt acttacttaa cattttccca tttggtacta
     1801 tctaacccct tttcactatt aagaagtaat gcctactatg actcaagtcg cgaagaaaat
     1861 tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc cacctcggcc atatgctgga
     1921 gcacatccag gctgatgtct gggtccgtta ccagcgaatg cgcggccacg aggtcaactt
     1981 catctgcgcc gacgatgccc acggtacacc gatcatgctg aaagctcagc agcttggtat
     2041 caccccggag cagatgattg gcgaaatgag tcaggagcat cagactgatt tcgcaggctt
     2101 taacatcagc tatgacaact atcactcgac gcacagcgaa gagaaccgcc agttgtctga
     2161 gcttatctat actcgcttga aagaaaacgg ttttattaaa aaccgcacca tctctcagct
     2221 gtacgatccg gaaaaaggca tgttcctgcc ggaccgtttt gtgaaaggca cctgcccgaa
     2281 atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc tgcggcgcga cctacagccc
     2341 gactgaactg atcgagccga aatcggtggt ttctggcgct accccggtaa tgcgtgattc
     2401 tgaacacttc ttctttgatc tgccctcttt cagcgaaatg ttgcaggcat ggacccgcag
     2461 cggtgcgttg caggagcagg tggcaaataa aatgcaggag tggtttgaat ccggtctgca
     2521 acagtgggat atctcccgcg acgcgccgta cttcggtttt gaaattccga acgcgccggg
     2581 taaatatttc tacgtctggc tggatgcgcc gattggctat atgggttctt tcaagaatct
     2641 gtgcgacaag cgcggcgaca gcgtaagctt cgatgaatac tggaagaaag actccaccgc
     2701 cgagctgtac cacttcatcg gtaaagatat tgtttacttc cacagtctgt tctggccagc
     2761 aatgctggaa ggcagcaact tccgcaagcc gaccaacctg tttgttcatg gctatgtgac
     2821 ggtgaacggc gcgaagatgt ctaagtctcg cggtaccttt attaaagcca gcacctggct
     2881 gaatcatttt gacgccgaca gcctgcgtta ctactacact gcgaaactct cttcgcgcat
     2941 tgatgatatc gatctcaacc tggaagattt cgttcagcgc gtgaatgccg atatcgttaa
     3001 caaagtggtt aacctggcct ctcgtaatgc gggctttatc aacaagcgtt ttgacggcgt
     3061 gctggcaagc gaactggctg acccgcagtt gtacaaaacc ttcactgatg ccgctgaagt
     3121 gattggtgaa gcgtgggaaa gccgcgaatt tggtaaagca gtgcgcgaaa tcatggcgct
     3181 ggctgatctg gctaaccgct atgtcgatga acaggctccg tgggtggtgg caaaacagga
     3241 aggccgcgat gctgacctgc aggcgatttg ctcaatgggc atcaacctgt tccgcgtgct
     3301 gatgacttac ctgaagccgg tactgccgaa actgaccgag cgcgcagaag catttctcaa
     3361 tacagaactg acctgggatg gtatccagca accgctgctg ggccacaaag tgaatccgtt
     3421 caaggcgctg tataaccgca tcgatatgaa gcaggttgaa gcactggtgg aagcgtctaa
     3481 agaagaagtg aaagcagctg ccgcgccagt tactggccca ctggcagatg atccgattca
     3541 ggaaaccatc acctttgacg acttcgccaa agttgacctg cgcgtggcgc tgattgaaaa
     3601 cgcagagttt gttgaaggtt ctgacaaact gctacgcctg acgctggatc tcggcggtga
     3661 aaaacgcaat gtcttctccg gcattcgttc tgcttaccca gatccgcagg cactgattgg
     3721 tcgtcacacc attatggtgg ctaacctggc accacgtaaa atgcgcttcg gtatctctga
     3781 aggcatggtg atggctgccg gtcctggcgg gaaagatatt ttcctgctaa gcccggatgc
     3841 cggtgctaaa ccgggtcatc aggtgaaata atcccccttc aaggcgctgc atcgacagcg
     3901 ccttttcttt ataaattcct aaagttgttt tcttacgatt ttgtctctct ctaacccgta
     3961 taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag aatgagacac
     4021 tttatctatc aggacgaaaa atcacataaa ttctgggcgg ttgagcagca gggaaacgag
     4081 ctgcatatca actggggcaa agtcggcact aacgggcaaa gccagataaa aagttttgcg
     4141 gatgttgcag cagcggaaaa agcgaaactg aagcttatcg cggagaaaac gaaaaaaggc
     4201 tatgtggaaa atgcttcagc aaacgtgcat atccccccca tcaccaaagc cactcctgaa
     4261 gttgaaactt cccctgagag taaaaaccaa cgcccgtggc tggctgatga tgcagttatt
     4321 ccggtaactg acgatattaa ccgatttgct tttcctcatc gctctcgccc cagagagatc
     4381 aattatctgc gcaaagacgg tgagatatgg aagcgtattg cagataacac cagggcatat
     4441 gatcccgaca acaattaccg ctcttatcct gaaaactggc agcaggcttt tgctgagtta
     4501 caaaggcgaa ttctggataa tcaacagaca gggagtgcgc aatctgatgc agcattgctc
     4561 tggagttttt ggaactctta ctctgcagat gaactggtcg atgacttagt cattcgctgt
     4621 ggtctggaaa gcgccgttga aatcgtcctt tttgcccttc aactcaaata taaaccagta
     4681 aaaggcgcag taaccaccat cattccacct aatcacgaag cagaatcgct acctagttag
     4741 catcaacgtc tatgtcatca tctttcactc gcttcagaag atgagtggca acgctgtgta
     4801 gataaagtac tcaccgccat tccgacgcta tcccccgcac ggcaaccttt tgcggcgtta
     4861 ctgctccctg aacgcccgga tatcgcccat gctatagatc tacgctatgc agaccaaaac
     4921 gtcccggcga tgacctggtt aagactggtg ataaccgatg atactgctct gagtacaatg
     4981 gaaaaatacg acttcccacc gttgtatcgt gattttcgta attttcgcgc ttatctggca
     5041 atgttgttgg caaataatgg ggtgcgcggt gtaagccgga tacttcttga gtttacggaa
     5101 gaccattctg acaatcccac ttatctcttc gaacgcatca gcgagactga aaatcttgtc
     5161 aaatggctat ggaaaacgaa tcacccggat gcgattcaaa ttctgatcct cggtgtaatt
     5221 ggtaagaaaa agcacctgga atacttaagc aaggccagtc aaaagcatcc cgctgcggct
     5281 attgcggctt atgctacttt gctggcaata catgaagata aagagtggcg taaagcgctt
     5341 gtcaaactga ttaccgccac gccagagtta gtatgcgacg tcattccctg ggttaatgct
     5401 aaagcggcag gcatcttatc tgaatgtcgt ccgcagtcag tcgctgaaga atgtgaatat
     5461 gccactgtgg atatgttgcc ggagctgctt gtttcgccgc cctggatgac caaagagaaa
     5521 aagaaaaata cgcctgtgtt tgatcttcca gtgcttccag ttccttctgt ttctgatgtc
     5581 actccagaga ttaccaaaaa actgacccgt acttaccttg tcacccactt ccagcaaata
     5641 gcccaacagc aggcgacaaa acaaacattg tttaccgacc tgccgccgat caaaaaagca
     5701 agctgggaga aacacttaat accgcttaca ccggagcaac aaatactgtg gcatcttggt
     5761 tttgaaaaat ggcgggagag cggagaaaaa atatacgaaa aaattccggc gccacaaagc
     5821 gccgttgatg ccctgctacg cttcgatttt ccagcactca atgctgaatt tgtgcattat
     5881 cataacaatg catacaaaag ctggaatctc attgccctct gttacctgcc tggtcaacag
     5941 gccatttctt ttcttaacca gatagtcaaa gaagataact actctggtga gggaaatatt
     6001 ctggcaattt ttggtagcgc cgccatacct gcatttatgg cctgcctgca acgtgatcca
     6061 cgacgtctgt gcttcttccc ctttttcctc ggtgtcagtg aactggcctt accgatggcg
     6121 caacaattac agaaaaaaat gtcttatgaa gatgcgcgaa actggctaac tgattatcca
     6181 cgccatgccg ccgccgggct gcttcctgtg gccctgggca aaaaaggaaa agatcgtgat
     6241 tgtgcccgcc aggcgcttcg tctgctggtg aatttaaacc agcgagaaac gattgaggaa
     6301 atcgcgcaag gatataatca acctgatgtt ttagctgcgc tggcaacatt gttcgatagc
     6361 gacccactgg aagaatatcc cgctaaaatc gccccactgc ccggctttta tcagttcacc
     6421 ttgtggcgca gaccacggct taaaagtaac aacctgcctc tgtcagatga cgctatgcgc
     6481 cacctcggca ctatgctgag ctttcctcgc gacattaccg cctacgctgg gctggatatc
     6541 attagagaaa tcttcacccg cgaatcactg gctgaatttg gctgggatct gtatcccgcc
     6601 tggacagaag ctggcgcacc cgcaaaagaa aactgggcat ttacttcgct tgggatttta
     6661 ggcaatgacg acaccgcgcg taaattaacc ccgcttatcc gcgcctggcc tggggaatcc
     6721 cagcataaac gggcggtgtc cgggctggat gtattagctg atattggcag cgatgttgcg
     6781 ctaatgctgc ttaatggcat cgcgcgaaaa attaaattca aagcattaca ggaacatgcc
     6841 cgcgagaaaa tcaacatagt tgctgaaaac cgtgggctga ctatggctga actggaagac
     6901 cgcctggctc cagatttagg gcttgatagc agtggctcgc tgatactgga tttcggcccc
     6961 cgcaaattca ccgttggttt tgacgaaacc ttaaaacctg tagtgtgcga tgcaaacggc
     7021 aaagtcctga aagatttacc taagccaaac cagagcgatg aaaaaactca ggcaactgac
     7081 gcggttaatc tcttcaaaca gttgaaaaaa gatgtacgcg ccatagccag ccagcagatt
     7141 gatcgtctgg aacaggctat gtgccagcgc agacgctgga cggcagagca gttccgcctg
     7201 tttctggtgg agcatccgct ggtacgtcac ttaacccgac ggctgttatg gggggtttat
     7261 aacgatgaaa acgcccttat cacctgtttt cgcgtggcag aagacagcac ttacagcgat
     7321 gcgcaggatg agttattcac gctgccagca ggaaacatcg gtattccgca tgtgctggaa
     7381 atatcccctg aatccgccgc tgcattcagg caaatttacg ctgattacga actgcttccc
     7441 cctttccaac agctcgagcg aggtagctat caccttgctg ataatgaacg taatactcac
     7501 gaactgacac gctggcaagg acggctttgc caggccggac gcattgtcgg gctggaacgc
     7561 agaggctggc aacgtctgga agaaagcggc agcgtttatg cgatgcgcaa aacaacccct
     7621 catggcgatc tcgaacttga aacggaacct ttttcattaa tttatggtga aacggggtat
     7681 ggcgaccagc acccggttga aagcgtaaaa atcacctcgc cagatgatcg ctacggtaaa
     7741 caatcctcac tcactttctc catgctcgac gacatcaccg ccagcgagct gattaacgac
     7801 attgaatcac tgtttgatta aaaggattta acaatgaaaa cctatattta tcaggacgaa
     7861 aaatcacata aattctgggc ggtggagcag cagggaaacg agctgcatat taactggggt
     7921 aaagtcggta ccaacgggca aagtcaggta aaaagctttg cagatactgc ggcggcggaa
     7981 aaagcagcac tgaagctgat tgcggagaag gtgaagaagg ggtatgtgga gcaagtagac
     8041 gctaacgctc actcacatga tgcctcagca aagaaagtcc tggtcacaga ggaaaaagcc
     8101 tcaataaatc gccaggcagc aactaatggt ttaccctggc tggcagacaa cgatcccatt
     8161 attcttcccc cgaatattgc ccgccatgcc ctcagccacc gcctttggcc gggagagcca
     8221 gtaaaaaaac caaccaaacc cgagaaacaa aatttaattt cccgattggc aaatcgtact
     8281 tatcattatt ataaaaatct gactttcgat tacagctcat gctctgatga atgggctgat
     8341 gcgattagcc aggcaatcag cttaattaag gcccaaaaga ctgagatacc cgcgcgtact
     8401 atggcggtac ttacttttct ggaaatatgt ttttgtcagg gctggaatga tgaaacctca
     8461 tccgagttgc tggatgaaat tgtaagcacc ttcggtatcg aatacgcaac tgaaactgtc
     8521 atcttctggt ggcaaattga tttcgatttt gactataaag atgaacattt gactttcttt
     8581 attaattatg cagatagcag caaggacagt tatagaagga ataacgacaa atttagttta
     8641 cgtctgcgta aacatttatc actagcagaa gaagaagtat ggcaaaattg cattgcaaaa
     8701 ttactcgttg cgttaccaga tatatcactc tatcgccaac cgttaattgc cattttaatg
     8761 ccagaaatac ccgaagtggc ccatgagatc gtacatcgtt tacaccaggt cgcagatgtt
     8821 ccacaacttg aaatgttgaa actggtagcg acagatccat tcacgctcga aatactggag
     8881 aactatcaat acattgatgt atttaacacc tatggtgcca gttggtcagc tacggtatta
     8941 cgggagcaag gtatagcggc actggctcgc ctgactccct atgctgaaga agataattgc
     9001 ggtgacgtac tgaaatgtat taatcatccg ttggccatta cgaataaaaa gtgccgaaaa
     9061 ttttccttct gcaacgatag tggcattagc cgaagcattg gtaaaaaaag atgataaacg
     9121 caaacataca ttgctggttt cattacttaa tgcaaatcct ttatttgtcg aaaaattagt
     9181 catcggatta tcgtcgcaag cggcaaattt attgcaacaa tgtcagcaat tgctgaatag
     9241 tcgcaatgaa gattttgcaa actcagaaat tctaccaatg gtactagtct cgccgccctg
     9301 ggaaactcaa aataaaaata acgacaacag tcctgtgggt aaatacccgg cgaaaatccc
     9361 ccctttaccg ttgttttatc aacctccatt atggactcgc ccgaaactca aaaatagcag
     9421 caaggtactg ccagatagcg cactccagca cctcggtgaa atgctccgct tccctcagga
     9481 agaggccctg tatccgggat tattgcaggt gaaagatgcc tgtaccgccg actcactggc
     9541 tgaatttgcc tgggatctgt ttaccgcctg gctgactgcg ggcgcaccgt cgaaagagag
     9601 ttgggcgttc actgcgttag gcgttcttgg taacgatgac accgcccgca aactgacgcc
     9661 attaatacgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg ggttggatat
     9721 tctcgccgct atcggtagtg atatcgccct gatgcagctt aacggtattg cccagaaact
     9781 gaaattcaaa gcattacagg agcgggcaaa agaaaaaatt gccggcattg ccgaaagccg
     9841 cgaactcacg gtggcagagc ttgaagatcg gttagcaccg gatctcggtc tggatgataa
     9901 cggttcgttg ctgctggatt tcggctcacg ccagttcacc gtcagctttg atgaaacctt
     9961 aaaaccgttt gtgcgtgatg tttctggcag ccgtctgaaa gacctgccca aaccaaacaa
    10021 aagcgatgat gaatcacagg cgaacgatgc tgttaaccgc tacaaactgc tgaaaaaaga
    10081 tgcgcgtacc gtcgccgccc agcaggtagc aaggttggaa tccgccatgt gcctgcgtcg
    10141 ccgctggtcg ccagaaaact ttcagctctt cctggttgaa catccactgg tacgccactt
    10201 aacccgccgt ctgatttggg gcgtttatag caccgaaaac cagctactga cgtgcttccg
    10261 cgtggcggaa gataacagct acagcaccgc tgacgatgat cttttcaccc tgccggaagg
    10321 cgatatctct gttggcattc ctcacgttct ggaaatatca ccgacggatg ctaccgcctt
    10381 tggtcagctt tttgccgact atgaactgct accaccgttc cgtcagcttg accgtaacag
    10441 ttacgccctg acagaagccg agcgcaacgc cagcgaactg acccgctggg caggcagaaa
    10501 atgcccgagc ggtcgggtca tggggctggc gaataaaggc tggataaagg gcaccccgca
    10561 ggatggaggc tggatcggct ggatgatcaa acctctgggc cgctggtcgt taatcatgga
    10621 aatcgatgaa ggttttgcgg taggcatgtc gccagccgaa ctgagtgccg agcaaatctt
    10681 aagcaagctg tggctatggg aaggcaacgc agaaagctat ggctggggga gtaattcaac
    10741 acaggaagcg cagttctccg tactcgatgc catcaccgcc agcgagctaa ttaacgatat
    10801 tgaagccctg tttgaataag gaaagagcat ggacaaggaa ttaccgtggc tggcggataa
    10861 cgcccaactg gaactgaaat ataaaaaagg caaaacgccg ctcagtcatc gtcgctggcc
    10921 gggcgaacca gtgtccgtta tcactggaag tctcatccag acattgggag atgaattact
    10981 acaacaagca ggccagaagg aaaatatcac ctggaattat gataaatgct cactggagtg
    11041 gcagtccgcc atccagcagg caatcaattt gactggcgag cataaaccgt caatcccagc
    11101 ccttaccatg gcggctctga tctgtatcgc gcaaaatgac agccagcagt tacttgatga
    11161 aatcgtgcaa caagaggggc tggaatatgc gacggatgta gtgatatcgc gtcagtgcat
    11221 tgcgcggcgt tatgagagtg actcactggt cgtcactttg cagtatcagg acgaggacta
    11281 tggctacggt tatggctccg cgacctataa cgatttcgat ctccgtttgc gtaagcatct
    11341 gtcgctggca gaggaaagtt gctggcagcg ttgtgccgat aaactcattg ccgcactgcc
    11401 aggaataccc aaaattcgcc gtccttttat tgcgctgatc ctcccggaaa aacctgaaat
    11461 agccaatgaa ctcgccagtc ttgaaagctc ccggtcaagt ctccattcaa aggagtggtt
    11521 aaaggtcgtt gctactgata atacggcggt aaaaaaactc gaacgatact ggggcctgga
    11581 cgtatttagc gatcgcgaag ccagctacat gtcacaagaa aaccgcttcg gctacgcggc
    11641 ctgcgcctcc cttttgcgcg aacaaggact ggcagccgtt ccgcgcctgg caatgtatgc
    11701 tcataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag tcatccgcac
    11761 cttgctgtta gttgctgata aaaacaaacc cagcctgcaa cgtgtggcta aatacagtaa
    11821 aaacttcccc catgcgacgc tcgccgcact ggcagaactg ctggcgttaa aagaaccacc
    11881 agcccgccct ggttatccaa tcatcgaaga caaaaagctg cctgcacagc aaaaagctcg
    11941 agaagaatac tggcgtacgc tgttacaaac gctgatggca tcgcagccac aactggcagc
    12001 agaagtgatg tcgtggttaa gtactcaagc cagggcagtg ctgaagagtt atttatcggc
    12061 actgccaaaa ccggttattg atggcaccga taacagcaat ctgcctgaaa tcctcgtttc
    12121 accaccgtgg cgtagtaaga aaaaaatgac agctccacgt cttgatttgg cacggctcga
    12181 attaactccg caagtttact ggcaaccagg cgaacgagag aggcttgccg ccactgagtc
    12241 tgcccgttat ttcagcacgg aatctcttgc gcaacgcatg gaacaaaaaa gtgggcgagt
    12301 tgtattacag gaactgggtt ttggggatga tgtatggctg tttttgaatt atatactccc
    12361 tggaaaactg gatgctgcac gcaattcact cattgttcag tggcattact accaggggcg
    12421 ggttgaagag atcctgaatg gctggaactc cccggaagca caattagcag aacaggcgct
    12481 ccgcagcggt cacatagaag cgttaattaa catatgggaa aatgacaact actcacgtta
    12541 tcgtccggaa aagagtgtct ggaacctgta tttattggca cagttgccgc gtgagatggc
    12601 tttgactttc tggctgcgta tcaatgagaa aaagcatctg ttcgcgggag aggactattt
    12661 tctcagtatc ctcggattgg atgcgctacc aggtctgctg ttggcttttt cacatcgtcc
    12721 aaaagaaaca tttccgttaa ttttaaattt cggcgcaaca gaactggccc tgcccgttgc
    12781 ccgcgtctgg cgacgtttcg cggcgcagcg tgatctggct cgccagtgga ttttacaatg
    12841 gccggaacat acggctactg cacttatccc tcttgtcttc accaaatcta gcgataaaag
    12901 cgaagctgca ttacttgccc tgcgtttact ttacgaacat gggcatggcg aattgctaca
    12961 aactgtagcc aatcgttggc agcgtacaga tgtatggcct gccctggagc atttactgaa
    13021 acagggtccc atggaaattt acccggcacg cattccaaaa gcccctgatt tctggcatcc
    13081 gcaaatgtgg tccaggccgc gccttatcac taataatcaa cctgttaccg atgacgctct
    13141 ggaaattatc ggcgaaatgc tgcgctttac ccagggggga cgtttttata gcgggctgga
    13201 acaactgaaa acgttctgcc agccacaaac gctggcagct tttgcctggg atctcttcac
    13261 tgcgtggcaa caagctggtg cccccgcaaa agacaactgg gcatttctgg cgttaagtct
    13321 ctttggtgac gaaagcacgg cacgggatct aacaacacag atcctcgcct ggccacaagg
    13381 caaatctgcc cgtgctgtca gcggcctgaa catccttacc ctgatgaata atgatatggc
    13441 gctgatacag ctgcatcata tatcgcaacg ggctaaatcc cgccccttac gtgataacgc
    13501 ggcggaattt cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agctagcgga
    13561 cagattagtc ccaaccctgg gccttgatga tccgcaggcg ttgagttttg attttggtcc
    13621 ccggcagttt accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa
    13681 cgttcgccag aaaagcgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga
    13741 ggcactggcc cgactaaaag ggctaaaaaa agatgctact caggtgagca aaaacctgct
    13801 cccgcgtctt gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc
    13861 tctgtttgtt aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttta
    13921 tccggcaaat gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggggtt
    13981 ctgcaatgcg caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca
    14041 cccgttagaa atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga
    14101 aattatgccg ccttttcgcc agttggcgcg ccgcacggtg ctgctcacac ctgatgagtc
    14161 aaccagtaac agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg
    14221 aatgcgatac aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga
    14281 gtaccggctg gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc
    14341 gttaatgagc ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga
    14401 acttgatgtg tttaatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa
    14461 acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg gggaaagcga
    14521 tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta cctgaagatc
    14581 cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt tccgtctatc
    14641 gactggagcg attccggcta cgttcaacat ttcatcaacg tgatcgagaa gatgctgacc
    14701 cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag cggaaattac
    14761 gaggtgaatc acctgaaact gtaatagcaa tttttaatcg tgaactacag aatatattca
    14821 accaagaaat agaataatat actctagata atta
//
LOCUS       DAZFKY010000118        14665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFKY010000118 DAZFKY010000000
VERSION     DAZFKY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(217..732)
                     /gene="pgpA"
                     /locus_tag="ACHKCL_003188"
     CDS             complement(217..732)
                     /gene="pgpA"
                     /locus_tag="ACHKCL_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5764099.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(710..1687)
                     /gene="thiL"
                     /locus_tag="ACHKCL_003189"
     CDS             complement(710..1687)
                     /gene="thiL"
                     /locus_tag="ACHKCL_003189"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5764100.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1765..2184)
                     /gene="nusB"
                     /locus_tag="ACHKCL_003190"
     CDS             complement(1765..2184)
                     /gene="nusB"
                     /locus_tag="ACHKCL_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5764101.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2204..2674)
                     /gene="ribE"
                     /locus_tag="ACHKCL_003191"
     CDS             complement(2204..2674)
                     /gene="ribE"
                     /locus_tag="ACHKCL_003191"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5764102.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2763..3866)
                     /gene="ribD"
                     /locus_tag="ACHKCL_003192"
     CDS             complement(2763..3866)
                     /gene="ribD"
                     /locus_tag="ACHKCL_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5764103.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3870..4319)
                     /gene="nrdR"
                     /locus_tag="ACHKCL_003193"
     CDS             complement(3870..4319)
                     /gene="nrdR"
                     /locus_tag="ACHKCL_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5764104.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4470..5009
                     /locus_tag="ACHKCL_003194"
     CDS             4470..5009
                     /locus_tag="ACHKCL_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5764105.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5308..6192
                     /gene="tsx"
                     /locus_tag="ACHKCL_003195"
     CDS             5308..6192
                     /gene="tsx"
                     /locus_tag="ACHKCL_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5764106.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6369..6716)
                     /gene="yajD"
                     /locus_tag="ACHKCL_003196"
     CDS             complement(6369..6716)
                     /gene="yajD"
                     /locus_tag="ACHKCL_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5764107.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6804..7085)
                     /locus_tag="ACHKCL_003197"
     CDS             complement(6804..7085)
                     /locus_tag="ACHKCL_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5764108.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7078..7320)
                     /locus_tag="ACHKCL_003198"
     CDS             complement(7078..7320)
                     /locus_tag="ACHKCL_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5764109.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7471..8442)
                     /gene="secF"
                     /locus_tag="ACHKCL_003199"
     CDS             complement(7471..8442)
                     /gene="secF"
                     /locus_tag="ACHKCL_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5764110.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8453..10300)
                     /gene="secD"
                     /locus_tag="ACHKCL_003200"
     CDS             complement(8453..10300)
                     /gene="secD"
                     /locus_tag="ACHKCL_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5764111.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10328..10660)
                     /gene="yajC"
                     /locus_tag="ACHKCL_003201"
     CDS             complement(10328..10660)
                     /gene="yajC"
                     /locus_tag="ACHKCL_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5764112.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10683..11810)
                     /gene="tgt"
                     /locus_tag="ACHKCL_003202"
     CDS             complement(10683..11810)
                     /gene="tgt"
                     /locus_tag="ACHKCL_003202"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5764113.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11865..12935)
                     /gene="queA"
                     /locus_tag="ACHKCL_003203"
     CDS             complement(11865..12935)
                     /gene="queA"
                     /locus_tag="ACHKCL_003203"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5764114.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13028..13609
                     /gene="acpH"
                     /locus_tag="ACHKCL_003204"
     CDS             13028..13609
                     /gene="acpH"
                     /locus_tag="ACHKCL_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5764115.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13630..>14665)
                     /locus_tag="ACHKCL_003205"
     CDS             complement(13630..>14665)
                     /locus_tag="ACHKCL_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764116.1"
                     /translation="KLCLKICNSCNQNDSSTHEYKIDHQQLKNHIRQLEEWALLEKFY
                     DNNAYYSKRTKQLEKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQISS
                     SGYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAK
                     QNAKSIEEKNIEKNKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIY
                     CLFDAFSQGLHNVARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISV
                     KSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCY
                     AAPLDANAKK"
BASE COUNT         3640 a         3900 c         3491 g         3634 t
ORIGIN      
        1 tcagagaata tgagcaatta ttatgagttc gtcgactgac gatatttcag tgtcattgaa
       61 ttatgagtga caaagataca ccttgaaata tcgccgcata gtatattcgg cgatgtcggg
      121 ttttacttta acagagtgcc ccaaaattgg ggcactgttc atttataaat caaaccgggt
      181 ggtctggaat cgcctacacc tcccggtcaa tggcatttaa agaataccca gcggccagtg
      241 atgaccgata aagtacagga tgcctgcgga aatcaccccg gcgacaatat catcgatcat
      301 gatccccatg ccgccatgta cattgcgatc aaaccagcgg atcggccacg gcttccacat
      361 atccagaata cggaaaatca caaacccggc ggcgacccac tgccagtcat tggtcggcag
      421 cgccatcagg gtgatccaca tgccaataaa ctcatcccat acgatgctac cgtgatcgtg
      481 cacccccatg tctttcgccg tttgatgaca aagatagacg ccgatacaga tccccaacat
      541 caccaccagc gagtagagct gccagggcaa aaaggtcatc agataccaga acggaatcgc
      601 cgccagcgat cccatcgtgc caggaacgat tgggcttaat ccacttccga atccgacagc
      661 aagcagatgc cacggattac tcatcttcag gcgacttttc gcgacatctt tatggcgtgg
      721 caaaatggtc atatcctttc cagtctaacg tgacaggttc gccgtcacga ataaaacaaa
      781 gcccttcgat atcggcggtc atttgcccga tacaggtaaa cggtacgccc agatgaccga
      841 gagccacatc cagcgcgcca cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt
      901 caccgccaga gagcgcccag cgcagcgcct gttccggtac aacatggcga gaaagcgcat
      961 cagaaaacgg cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct ttcacgatat
     1021 gcccgagatc ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca
     1081 gtgcctgccc ctgtaaaata cgcggcgatg gacggagatg acgtttgatc aagtagtccg
     1141 catctttagc atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat
     1201 cgcccggtgt accggtcaca tagatccagt cacccggttt cgccccagag cgcgttaagg
     1261 ctcgtcccat cggaacaaag ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg
     1321 tatcgccgcc aatgagttgc atatcgtaat aattgagaag atcaaacaaa ctgtcgctga
     1381 aggactcaag ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc agccaggccg
     1441 gatcggcccc catcgctgcc agatcgctta ggttcaccgc cagtgcttta taagccagat
     1501 cagcaggatc gatatcaggg aggaaatggt tgcccgccac cagcgtatca gtgctgatcg
     1561 ccagggtctg tttctcgggg atattgagaa gagcgcaatc gtcgccgatg cccagttcga
     1621 catcaagacg agaacttctt acacggtcaa aataacgggc aatcagggag aactcgccac
     1681 atgccatacg ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcgc ggaacagtct
     1741 tccgtgaatc taccggcctg gatattactt tttgttaggg cgaatcacag gtgctgcttt
     1801 atcgagtacg ccgttgacga acttatggct gtcttctgcg ccgaacgatt tcgccagttc
     1861 gatcgcttcg ttaatggcca ctttgtatgg cacatcgcta cgtttagaca gttcgtacag
     1921 cgcaatgcgc agtactgctt tttctacctg tcccagttct tccagcaggc gggacaggta
     1981 tggcttcatc agtccgtcga ggtatgcggt attagtcgcc accccggcca gcagctcacg
     2041 gaagtacagg acgtcaacgt cttttacatc ctgttcagcc aggaactggt attcaacatc
     2101 agcgatgtcg ttctgggaca actgccagga gtagagcgcc tggacggcac actcacgagc
     2161 gcggcgacga gcagcaggtt tcacggattt ccccttacta atttcaggcc ttgatggctt
     2221 tcaatacatt aatcatttca agcgcggtca gtgcagcttc tgcacctttg ttgccagctt
     2281 tggtgccagc acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa
     2341 ccggaatttc gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat
     2401 attcaaaatg ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt
     2461 taccggtttt agccagtgca cccgccgcca gcggcagctc ataggcacca ggcacccaaa
     2521 caacggtaat gttttcatct tttacctgac cgatacgttt cagtgcatca attgcacctt
     2581 ccagcaggct gtcattgata aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt
     2641 ccggggtagc aacgttagct tcaataatgt tcattttctt ccttcgggtt cgagtatggc
     2701 cccgcagggg ggcggatttt agcataatat ttcgtgcgct gcttcccttt cgagccggga
     2761 gatcatgcac ccactaaatg caggcaaaca tccgggccta catgacgtat ctctttgaat
     2821 ttaaattggg gggcgtcagc taatttctca agccctggca gtgtgcataa tccacgggcg
     2881 tcgctgccta atagtttagg tgcgatatag acaatcagct catcgactaa ccccgcctgc
     2941 agcaatgcgc cagcgagcgt tggccccgct tccacccaga tgctgttaat ctgctgttta
     3001 cccagttgca tcatcagtac aaccagatcc agatgacctt tatgctctgg aatcagcaag
     3061 gtacgcaccg tttccggcca ctcacgagaa tcttcctgcg tacgcgcgaa ccaggtttcg
     3121 ccgggctgct gcacaatgtg atgttccggc gtcacgcgat tttggctatc aatcacaata
     3181 cgtaccggct ggcggagatg atgttgcggg tagagcgcct gagtttgtac atccagttca
     3241 gaccaacgca ccgttaaggc aggatcatcc gccagcaccg tagcgctgct ggttaaaatg
     3301 gcatgacttt gtgcgcgcag tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac
     3361 tggctttcgc cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga
     3421 atataaggaa agccggtgcg catccgcttg agaaagcctt tattcaattg ctcggcttca
     3481 ctcatcatca ggccgtggct gacgtcaatg ccagcctgtt gcagacggta aagtccacgc
     3541 ccagcgacct gcgggttcgg gtcctgcatc gcagcgacca cgcgcgctac gccagcggcg
     3601 atgagtgcgt cacagcacgg tggcgtacga ccatgatggc tacagggttc gagtgtgaca
     3661 taggcggtcg cacctttggc tttttcaccc gccatacgca acgcgtgtac ttcggcatgt
     3721 ggttcacccg ctcgttggtg gtaaccttca ccgacaattt cgccatcttt gacaatgacg
     3781 cacccgacat tcgggttggg gtgggtggta aaacgtcctc gttgcgccag ctttagcgcc
     3841 cgcgccatgt aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca
     3901 aattctttga tatcttcgaa actgcggtag acagaggcaa aacggatata ggcgacttta
     3961 tcgagctttt tcaattgctc catcaccaga ttgccaatca tcttgctcgg cacttcgcgc
     4021 tcaccggtgg cgcgcagctg cgatttaata tgattgattg ccatttcgac gtcatcggaa
     4081 ctcaccggac gtttttccag cgcccgcagc attccgctac gtaatttttc ttcattaaac
     4141 ggttcacgca cgtcgttgct ttttacaaca cgcggcataa ccagctccgc cacttcaaag
     4201 gtggtgaaac gttcattaca caccagacac tgccgacggc ggcgtacgga tgaaccctcg
     4261 cccacgagac gagagtcaat taccttagtg tccacggcga aacagaatgg gcaatgcata
     4321 cggcgtcctg accaggtggt taacggaaat ctattttacc ctgaactgac gatacaacaa
     4381 aggcgcaacg cttttgagat aaaggatcga tgctttcgct gcttttaccc tctaccatta
     4441 ggctaattgc gacgagaaag ggacgaccta tgaatattaa cgtttttcga ctgatcctgc
     4501 tgggaagcct attcagcctt agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac
     4561 acagcgtcag cacgctgaac caggagatga cgcaactcaa tcaagaaaca gtcaaaatca
     4621 ctcagcaaaa caggctgaat gcaaaatcca gcagtggggt ttaccttctg cctggagcga
     4681 aaacaccggc aagactggaa agccagatcg gtactttacg tatgtcgctg gtgaatatta
     4741 cgcctgatac agatggcacc accctgacac tacgtattca gggtgagtcg aatgatccct
     4801 tgcccgcatt cagcggaact gttgaatatg ggcaaattca gggaacaata gacaactttc
     4861 aggaaatcaa tgtacagaat caattgatta atgcccctgc cagcgtcctc gcccccagcg
     4921 atgttgatat tccgttacag ttaaagggga tttctgtgga tcagttaggc tttgtacgta
     4981 tccacgacat tcaacctgtt atgcagtaaa cttatagcgg ggcgattttg tgcgtcccgc
     5041 aacatccttc cccatcattt tgttactccg cttacataac ccagattgat agtaaaagtt
     5101 tgcaacaagg gcgaaagtca gtacaatccc cgcccgaatg tgtgtaaacg tgaacgcaat
     5161 cgattacgta aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat
     5221 aataggctcc tctgtatacg aaatatttag aaacgcaatt tgcgcctttt tcactcccgc
     5281 aagggatttt caaacagtgg catacatatg aaaaaaacat tactggcagc cggtgcggta
     5341 ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc gcagtatctt
     5401 tccgactggt ggcaccagag cgttaacgtt gtcggaagct atcacacccg tttcggaccg
     5461 cagatccgca acgataccta ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac
     5521 ttctatggtt atgcggatgc gccggtattc ttcggcggta actccgatgc aaaaggtatc
     5581 tggaaccacg gttctcctct gtttatggaa atcgaaccac gtttctccat cgacaagctg
     5641 accaatactg accttagctt cggtccgttc aaagagtggt acttcgcgaa caactacatt
     5701 tacgacatgg ggcgtaataa agatggtcgc cagagcacct ggtacatggg tctgggtacc
     5761 gatatcgaca ctggcctgcc gatgagcctg tccatgaacg tctatgcgaa ataccagtgg
     5821 cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa aattaaatac
     5881 tttgtgccga ttaccgatct gtggggcggt cagctgagct acatcggctt caccaacttc
     5941 gactggggtt ccgatttagg ggatgacagc ggtaacgcaa tcaacggtat taagacccgt
     6001 actaataact ctatcgcttc cagccatatt ctggctctga actacgatca ctggcactac
     6061 tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga acgacgatgc agaactgaac
     6121 ttcggcaacg gcaacttcaa cgttcgctct accggctggg gtggttacct ggtagtaggt
     6181 tacaacttct gattatgaaa atgccgggat ttattcccgg catttcagat tgttaacaac
     6241 gtacgctttg ttcatgccgg atgcggcgta aacgctttgt ccggcccaca aaagcacgta
     6301 aattcaatat attgctgaaa taacgtaggc ctgataagcg tagcgcatca ggcaatttta
     6361 cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc
     6421 gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc
     6481 gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta
     6541 ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc
     6601 aggttggaat aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat
     6661 gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaaa
     6721 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
     6781 tcctgagtca agaaattctc acattaccag tgacggcgag gttccatcaa ctgatgaaga
     6841 atgcgcgcaa tgagaatgtc atcgactctt ataacgtaat aaacagtgtg aaacctaaat
     6901 gcgatcttct ttacctctgg aatcgcatca tagtcttgcc ctatctcggg catttgagag
     6961 agtgtatcca gaaaagtgcc gagagagtcg gtatagcgat ccgcctgctc atcaccaaac
     7021 tttcccagcg tgtagtcata gatccctctg aaatcttcaa cagcttttcc gctaagctta
     7081 tacacgatgt ttttgcttcc tttctgctgc gatatcacga agcgtcatcg aactttcacc
     7141 gctctcaaga ccttcaataa cagcatttct gacagcttca tccctggatt cttgttgttc
     7201 caatagacga agtgccgaac gcattacttc actggccgag ccataacgac cagactggac
     7261 catccgggta ataaaactat ctaattgttc gccaacgctt actgaggtaa ttttgcgcat
     7321 aagcgatctc cacgtattaa ctattaatac aaagtatatg tcagaacgct ttctacaacc
     7381 aaaccgtatt cagttgcctg tgaaacaggc aaaaaaaacc gcagcgcaaa tacgccacgg
     7441 tttttttaac agaacggatt taaaacacca ttacggcaga attgacggct gatccgcccc
     7501 ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa
     7561 cgcagatgcc acatagatgg aagatgcagt accgatggaa acaccgataa gcatggtcag
     7621 cgagaagcct tccagtaccg gaccaccgaa gagatacagc atcaggataa ccatcaaggt
     7681 agtaccggat gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt taaagatttc
     7741 gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga attcggtccg ataccacgat
     7801 actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat
     7861 ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat
     7921 aaccacccct gccgccagtc gccactcaaa gcggaaacct acgtacacga ggatagacag
     7981 cagcgctgcc atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc
     8041 gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag
     8101 aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat
     8161 gatgtcgtgg ctgctaccaa agttttgcag catcggctct tcaaaaccgg ctttttgcaa
     8221 tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc
     8281 accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga tagcagcgat
     8341 taacagcaga ccagagatgc caaaagccca gtagtcccag cgcataaagt catagacttt
     8401 acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg
     8461 acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa
     8521 tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg
     8581 ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga
     8641 agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt
     8701 tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa
     8761 gggttaagac gatacccgcg atacctggca tactcagtgt tgcgcctggc aacagcgaca
     8821 taataccgac gatcaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact
     8881 tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca
     8941 gcccctgctc aatgttctgc atacccaggg tcgggccaat tgtacgttct tcaacaatct
     9001 gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg
     9061 ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg ttggcgatgt
     9121 taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttaccgctgt
     9181 ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtccttg gtgaagttag
     9241 acataatgtt gccaccagcg ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt
     9301 cctggctgga agtggagtcg gtgatatggt caccggtaag aattacgcgt ttgtacagca
     9361 caaccggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg
     9421 ccgcagcggc ctgatcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac
     9481 ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt
     9541 cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca
     9601 ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca
     9661 ttaccgcacg cagctggttg ctgccctgac tgctaatcac caggtccgga tgacgcttgc
     9721 tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt
     9781 agttattttc tttacgaacg gtggtatacg ggatgccttt ttcgcgcagg tcgctgcgca
     9841 ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca
     9901 ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg
     9961 ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg tcacccataa
    10021 cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa
    10081 tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct
    10141 ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag
    10201 ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga
    10261 tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa
    10321 attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt
    10381 taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt
    10441 tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt
    10501 ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga
    10561 aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg
    10621 cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa
    10681 tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg
    10741 aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag
    10801 taacgaaggt tatgaatggt attgagtcgc gcgcctaata tttcgttgca acggtcaaga
    10861 tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt
    10921 gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac
    10981 aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga
    11041 cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct
    11101 gccggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc
    11161 acagccagac cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa
    11221 atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt
    11281 ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag
    11341 cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata
    11401 tccgaaccaa gatcgtactg aatctccatc gacttttccg ggtcgaggaa aatcgggtcg
    11461 ccgttgatcg ggttacggaa gtgcacgccc tgttcggtga ttttacgaat atcgccaagg
    11521 ctgaagactt ggaagccgcc ggagtcggtg aggatcggtc ccttccactg cataaaatcg
    11581 tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg
    11641 ttgccgagga taatttgcgc gccagtggct tcaacttctt ccggcgtcat cccttttacg
    11701 gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccacg atcaaagacc
    11761 aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca
    11821 ctacgtcaga aaaacagtcc aacgtttaaa ccagcgccgc ggaattactc cccgacgcgc
    11881 tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga
    11941 tatttctctt ctaccgccgc tttataggca ttcattgtgt gttgataacc ggcaaaggcc
    12001 gaaaccagca taatcagcgt cgattccgga aggtggaagt ttgtcaccag cgcatcgacc
    12061 actttgtact ggaagcccgg atagataaag atttgggtat cgtcaaagaa cggttcgatg
    12121 agatcgtttt tcgccgcctg agccgcgctt tccagcgaac gtactgaggt ggtgccaacc
    12181 gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggt
    12241 acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg taccggctgg
    12301 aaggtgcccg caccaacgtg cagggtgacg aacgccatct ccacgccttt ggcgcgtaat
    12361 ttttccagca aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt
    12421 ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata
    12481 tacggcggca gcggcatatg gccgatgctg ttgagaatat ccagcaccga gcgttcatca
    12541 ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg
    12601 tcatcgccca gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca
    12661 agaatgcgtt tgtcgtcgag catccgttca accagcactt caatcttgcc gccgctggct
    12721 ttacgcccaa acaggcgcgc cgggatcacc cgggtattat taaaaaccag aagatcgccg
    12781 gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg
    12841 tccagcgaca gtaaacgaca gctactgcgt tcaggcatcg gatagtgggc aatcagggat
    12901 tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga
    12961 ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt tacccccctt tggatagaat
    13021 ataatcgatg aattttttag ctcacctgca tttagcccat ctcgcggaaa gctcgctttc
    13081 cggcaatttg ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt
    13141 cgtggctggc attcatatgc atcgacgtat cgacgtattg actgacaatc tgccggaagt
    13201 ccgcgaagca cgggagtggt ttcgtagtga aactcgccgc gttgcgccta ttacgctgga
    13261 tgtcatgtgg gatcactttc tttcccgtca ctggtcgcag ctgtcgccgg actttccgct
    13321 acaggaattt gtttgttatg cccgcgagca agtgatgaca attttgccgg actcaccgcc
    13381 acgctttatc aacctgaaca actacttatg gtcagagcag tggctggtgc gctatcgcga
    13441 tatggatttc atccagaacg tgttaaacgg catggcaagc cgtcgcccac gtctggatgc
    13501 cctgcgtgac tcctggtacg atttagacgc ccattatgcc gccctcgaaa cccgcttctg
    13561 gcagttttat ccacggatga tggcgcaggc gtcacgcaag gcgctctgaa acttcaaact
    13621 ccggcgtcac tattttttag cattcgcatc cagcggggcg gcataacaac ggtaagaaaa
    13681 acattcgctg gaatgattgg cgatatcttt aaagttgctt tcagcaaccc cctcgaacat
    13741 gagtaccgtt ctggacaact ctccgttttc atttatatgt ggaaaatcaa attggctact
    13801 gaatcgccat acatctgtct tttctggctc gctcttcaca gagatatgtc gatggaggac
    13861 ccctcctgta aacgtgcgcc agaacaattg ttgatcaaaa tcttgtaata tcgcagactt
    13921 ggatgctatt tgacgtagta aacctgcata ccgtggactg ccattacgag caacattatg
    13981 caaaccctga ctaaaagcat caaacaaaca atagatgtga tcactagtga aaaaaggtct
    14041 taattttttt tgttcttcat cgctgtaatc caggagcatc tcattgattt cttccggtga
    14101 gaattttaag acaaaattat cctcagaatt tgtttgtttc ttgttttttt caatattctt
    14161 ctcctcaatg ctcttcgcat tttgtttcgc aattatttcc gcagtttttt tcgcaatttc
    14221 ttccgcaatt ttttcccgag ttattgattt tagactaaca ccatcgatgt caaccggtgt
    14281 tgttttcaga tctctctgcg cgtcttccaa atttgtctta tagagtttgt gcagtttatc
    14341 ggcagatacg tatccggatg aagaaatctg gtgtgtacgc atggggggct gttcacaaag
    14401 catcgaggct atatcgtctt taaactcttt tacattaaag ttctcggctt taaccttcct
    14461 tgttataaac cccgcatcgg caattttctt ctctaattgt tttgtacgtt tagaataata
    14521 agcgttgtta tcataaaact tttcaagaag ggcccactcc tcaagttgcc ttatgtgatt
    14581 tttcaactgt tgatgatcta ttttatattc atgtgtacta gaatcatttt ggttgcagga
    14641 gttacagatt tttaagcata atttt
//
LOCUS       DAZFKY010000119        14197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFKY010000119 DAZFKY010000000
VERSION     DAZFKY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(42..118)
                     /locus_tag="ACHKCL_003206"
     tRNA            complement(42..118)
                     /locus_tag="ACHKCL_003206"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Arg,seq:acg)
     gene            complement(181..257)
                     /locus_tag="ACHKCL_003207"
     tRNA            complement(181..257)
                     /locus_tag="ACHKCL_003207"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Arg,seq:acg)
     gene            complement(322..398)
                     /locus_tag="ACHKCL_003208"
     tRNA            complement(322..398)
                     /locus_tag="ACHKCL_003208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Arg,seq:acg)
     gene            complement(402..494)
                     /locus_tag="ACHKCL_003209"
     tRNA            complement(402..494)
                     /locus_tag="ACHKCL_003209"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Ser,seq:gct)
     gene            complement(810..995)
                     /gene="csrA"
                     /locus_tag="ACHKCL_003210"
     CDS             complement(810..995)
                     /gene="csrA"
                     /locus_tag="ACHKCL_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5764117.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1230..3860)
                     /gene="alaS"
                     /locus_tag="ACHKCL_003211"
     CDS             complement(1230..3860)
                     /gene="alaS"
                     /locus_tag="ACHKCL_003211"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5764118.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3988..4488)
                     /gene="recX"
                     /locus_tag="ACHKCL_003212"
     CDS             complement(3988..4488)
                     /gene="recX"
                     /locus_tag="ACHKCL_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5764119.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4556..5617)
                     /gene="recA"
                     /locus_tag="ACHKCL_003213"
     CDS             complement(4556..5617)
                     /gene="recA"
                     /locus_tag="ACHKCL_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5764120.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5697..6194)
                     /gene="pncC"
                     /locus_tag="ACHKCL_003214"
     CDS             complement(5697..6194)
                     /gene="pncC"
                     /locus_tag="ACHKCL_003214"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5764121.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6339..7424)
                     /gene="mltB"
                     /locus_tag="ACHKCL_003215"
     CDS             complement(6339..7424)
                     /gene="mltB"
                     /locus_tag="ACHKCL_003215"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5764122.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7679..8241
                     /gene="srlA"
                     /locus_tag="ACHKCL_003216"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7679..8241
                     /gene="srlA"
                     /locus_tag="ACHKCL_003216"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8238..9199
                     /gene="srlE"
                     /locus_tag="ACHKCL_003217"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             8238..9199
                     /gene="srlE"
                     /locus_tag="ACHKCL_003217"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            9210..9581
                     /gene="srlB"
                     /locus_tag="ACHKCL_003218"
                     /gene_synonym="gutB"
     CDS             9210..9581
                     /gene="srlB"
                     /locus_tag="ACHKCL_003218"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5764123.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9585..10364
                     /gene="srlD"
                     /locus_tag="ACHKCL_003219"
     CDS             9585..10364
                     /gene="srlD"
                     /locus_tag="ACHKCL_003219"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5764124.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10470..10829
                     /gene="gutM"
                     /locus_tag="ACHKCL_003220"
     CDS             10470..10829
                     /gene="gutM"
                     /locus_tag="ACHKCL_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5764125.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10896..11669
                     /gene="srlR"
                     /locus_tag="ACHKCL_003221"
                     /gene_synonym="gutR"
     CDS             10896..11669
                     /gene="srlR"
                     /locus_tag="ACHKCL_003221"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5764126.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11662..12627
                     /gene="gutQ"
                     /locus_tag="ACHKCL_003222"
     CDS             11662..12627
                     /gene="gutQ"
                     /locus_tag="ACHKCL_003222"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5764127.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12624..14138)
                     /gene="norR"
                     /locus_tag="ACHKCL_003223"
     CDS             complement(12624..14138)
                     /gene="norR"
                     /locus_tag="ACHKCL_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5764128.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3303 a         3930 c         3555 g         3409 t
ORIGIN      
        1 aaatccatct taaccatcat cactgatgat tacggagaat atggtgcatc cgggaggatt
       61 cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct acggatgcat
      121 cgggaaattt attttactgc tgatattgat accgctacta aagctgtatc aagtaagaga
      181 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
      241 agctgagcta cggatgcatc gggaaactta ctttactgca gattttttga taccgctact
      301 aaagccgtat caagtaagag atggtgcatc cgggaggatt cgaacctccg accgctcggt
      361 tcgtagccga gtactctatc cagctgagct acggatgcaa atggcggtga ggcggggatt
      421 cgaaccccgg atgcagcttt tgaccgcata ctcccttagc aggggagcgc cttcagcctc
      481 tcggccacct caccacacgc ctcttacgag tgcttcgaag aacttgttta tcgctcatcg
      541 tcgctgcgtg gcgcacatat tactttctga gacttataag tcaaacaatt tttcccacac
      601 ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa aacgtcaaaa agagtgtttt
      661 atctacagaa aaacggaggt ctggcagata gcagtaatgc aaaaaaatgg agacttaagt
      721 tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag gagaaatttt gagggtgcgt
      781 ctcaccgata aagatgagac gcggaaagat tagtaactgg actgctggga tttttcagcc
      841 tggatacgct ggtagatctc ttcacggtga acagaaactt ccttcggggc atttacgcca
      901 atacgtacct ggttgccctt tacccctaaa actgtcacgg tgacctcatc cccaatcatg
      961 agggtctcac caactcgacg agtcagaatc agcattcttt gctccttgaa agattaaaag
     1021 agtcgggtct ctctgtatcc cggcattatc catcatataa cgccaaaaag taagcaatga
     1081 caaacacatt acatgtaagc agtcatggca ttacattctg ttaaacctaa gtttagccga
     1141 tatacacaac ttcaacctga ctttatcgtt gtcgatagcg ttaatgcgaa tgccgtgaag
     1201 cgagtccacg gcattgcctg acgcttatat tattgcaatt tcgcgctgac ccagcctttc
     1261 acactggcta acgctgcagg taaggccgca gcatccgtac caccggcttg cgccatgtca
     1321 ggacgtccac cacccttgcc gcccacctgc tgagcgacca taccaatcag ttcccctgct
     1381 ttcacacgat ctgtgacgtc cttagatacg cctgcaatca gagaaacctt accttcggct
     1441 accgttgcca gcacgataat tgtcgacccc agctgatttt ttaaatcgtc aaccatggta
     1501 cgcaacattt tcggctcaac accgctaagc tcgctaacca acagcttaac accattaaca
     1561 tcaattgcct tactggaaag atttgcgctc tcctgtgcgg cagcttgttc tttaagctgt
     1621 tgtagttctt tttctaactg acgcgtacgt tccagtactg agcgcacttt atccgccaga
     1681 ttattgctat cgcctttcag cagatgcgcg acttcgctta agcgatcgct gtctgcatga
     1741 acggtggtga tagcgccttc tccggttacc gcttcgatac gacgaacgcc tgcagcagta
     1801 cccgattcag agatgatgcg gaacagacca atatcaccag tgcggctggc gtgagtaccg
     1861 ccacacagct cggtggagaa atcgcccatg ctcagcacgc gtacacgctc atcatacttc
     1921 tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt cgagatccat gatgttggtt
     1981 tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt cttcgaccgc acgaatctct
     2041 tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca gcaccttgtc gttaaccagt
     2101 gaacctttct gcgatacatg agtccccaga acctggcgca gcgcagcgtg catcaggtgc
     2161 gttgcggagt gattcaaacg aatacgggcg cgacgagcct catcaacatc agcctgcaca
     2221 gcgtcgccca ctttcagaga acccgcagca agtttaccga tgtgaccaat cgcctggccg
     2281 tatttctgcg tatcttccac cacgaaggag aagttagcgc ctttcagttc gcctttatca
     2341 ccaacctgac cgccggattc cgcatagaat ggcgtttgat ccagcacgac cacagcttcc
     2401 tggcctgcat tgatggcatc aaccgcttta ccatcaacaa acagcgcggt cactttgccg
     2461 ttcagttcca gatggtcata gcctttaaat tcagatgcac tgtcaacacg gatcattgcg
     2521 ttgtaatcgg caccaaagcc gctggcttcg cgcgcccgac gacgctgctc ttccattgct
     2581 gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac gacaaacatc agccgtcagg
     2641 tcaaccggga agccataggt gtcgtacaga cggaaagcag tttcaccatc cagcgtatca
     2701 ccagaaagtt ttgccagctc ttcatccagc aacgccagac cgcgctccag agtacgagca
     2761 aactgctctt cttcagtctt cagcacctgc tcaacctgcg cctgctggcg tttcaggtct
     2821 tcacccgcag agcccataac gtcgatcagc ggaccaacca gtttgtagaa gaaggtttct
     2881 ttcgcgccga gcatattacc gtgacgcact gcgcgacgaa tgatacgacg cagtacataa
     2941 ccacggtttt cattggacgg cattacgcca tccgcgatca ggaacgcaca agaacgaatg
     3001 tggtcagcga ttacgcgcag cgatttattg ctcagatcgg ttgcgccagt gactttcgct
     3061 accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt tagagttaac gtgttgcagc
     3121 accgcagcaa tacgctccag acccataccg gtatctacag acggcttcgg cagcggttcc
     3181 atcgtgccat cggcctggcg gttgaactgc atgaagacga tgttccagat ctcaatgtag
     3241 cggtcgccgt cttcttccgg acttcccgga gggccacccc aaatgtggtc gccgtgatcg
     3301 tagaagattt cggtgcacgg gccgcacgga ccagtgtcac ccatctgcca gaagttgtca
     3361 gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa tacgttcgcg cgggatccct
     3421 acttcttttt cccagatttc gtaggcttcg tcgtcgcttt catagacggt aacccacaga
     3481 cgctctttcg gcagggcaaa ccatttttcg ctggtcagca gttcccatgc aaactgaatg
     3541 gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca gcatttcgaa gaaggtatgg
     3601 tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt taccacccgc acgcacgcag
     3661 cgttgggaag tggtagcgcg ggaataatta cgcttgtcga gcccaaggaa cacatccttg
     3721 aactggttca tcccggcgtt ggtaaacaac aaagttgggt cgttatgggg taccagggag
     3781 ctgctggcaa ctacctgatg tcccttacta tggaaaaagt cgagaaacgc ctgacggatc
     3841 tcagcggtgc tcttgctcat aattatcctg aaatcaagct aacgaaatat cgccaccagc
     3901 tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg ggaataagat aagttttctt
     3961 gactgggaag taaaataccg tatgcgttca gtcggcaaaa tttcgccaaa tatcctggat
     4021 atcttccatc agatagccac gatagagcag aaaacgctgg atcttaactt tttctgaaaa
     4081 gacagttggc aaaggttcac catattttcg cgtcgcctga tcgcgcgcca gtgcgcacca
     4141 gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg cgggaaatac ctttctgatt
     4201 cagttcctgg cgaatacgcg caggtccata acctttgcgg ctacggctgg cgataaagcg
     4261 cgcaacaaat cggctgtcat cgagatagcc atgttcatgg caccaggcaa taacgcgctc
     4321 gtaatcttct gccgtagcat caatctcttc tgggccattt ttgcccataa tcggtgccgc
     4381 gagtttacgt cgcagttctt gctcactgtg atcgcgcacc gccagaatgc gtaccgcacg
     4441 atccaacagg cgagcatatg ccgggcgacg ggacgttgat tctgtcatgg catatcctta
     4501 caacttaaaa agcaaaaggg ccgcagatgc gacccttgtg tatcaaacaa gacgattaaa
     4561 aatcttcgtt agtttctgct acgccttcgc tatcatctac agagaaatcc ggcgttgagt
     4621 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4681 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4741 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4801 cgtagaagtt gataccttcg ccgtagagga tctggaattc agcctgttta aacggcgcag
     4861 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4921 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4981 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     5041 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     5101 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     5161 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5221 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttgt
     5281 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5341 acgcacaggt tttaccttca cgctgcgctg cggcaatcac ctgcaacgtc agcgtggttt
     5401 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5461 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5521 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5581 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5641 ataccggata gtcaatatgt tctgttgaag caattatact gtatgctcat acagtatcaa
     5701 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5761 accgcatcac ggtcgccgct gaagcattcc cgcagggtaa tgccttcacc gcgggcagtg
     5821 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5881 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5941 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     6001 atcatttgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggaa
     6061 ctgccggcaa tatcggtaat cactttcgct acccaaccgc cggtacaaga ctcagcagtt
     6121 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6181 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6241 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6301 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6361 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tactgtggtt ataacgggta
     6421 atggtgtaga agttcggcag accgtaccag tactggtaac ctgttccaac atccagacgc
     6481 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgcggca
     6541 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6601 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata attcgccacg
     6661 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6721 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6781 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6841 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6901 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6961 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     7021 ttccagaaaa ccacaccgtt ctgcacattg tccggcgtaa taaatttttt gcgataacgc
     7081 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     7141 atcagccgca gtaccgaatc cagacgtttc gcctgggaga gaatttcctg caactgctga
     7201 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7261 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7321 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7381 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7441 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7501 ctgcccgata cggactttac ataactcaac tcattctcct cgctatcctt ttactcaaac
     7561 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacacttta aatatttcaa
     7621 tctgattaga ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat
     7681 gatagaaacc attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga
     7741 ggtgtttacc gggatggtga ccggcattct tccgctgttg attagcctgc tggttatcat
     7801 gaacgcactg attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc
     7861 cggtaaccct gtttcccgtt acctactgtt accgtgcatt ggcacgtttg tcttttgcaa
     7921 tccgatgacc ttaagcctgg gtcgctttat gccggaaaag tacaaaccca gctactacgc
     7981 ggcggcctct tatagctgcc actcaatgaa tggcctcttc ccccatatca accctggcga
     8041 actgtttgtt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca
     8101 ctggcggtga gctatctgct ggttggtctg gtcaccaact tcttccgcgg ctgggtgacc
     8161 gatctgacca ccgctatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcat
     8221 ctggcaggag caacatcatg acgcatattc ggatcgaaaa aggaacgggg ggctggggcg
     8281 gcccgcttga gctggaaacc acgccgggca aaaaaatcgt ctatatcacc ccgccggtac
     8341 ccggcctgcg attgtcgaca aactggcaca gcttactggc tggcaggcta ttgacggatt
     8401 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8461 attacgctgc ggcatttatc cgaaacggcg tattcccacc attaatatcc actcgacggg
     8521 caagtccggt ccactggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8581 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8641 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8701 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8761 aaaaaccatt cttccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8821 tggccttggt gactggattg cccacggcct tgctccgctg gcgagccatc cactgggtct
     8881 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8941 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     9001 gccgcatctg gctttacctg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     9061 tccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     9121 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9181 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9241 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9301 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9361 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9421 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9481 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9541 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9601 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9661 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9721 ttaacgccga atatggtgaa ggaacagcgt atggttttgg tgctgacgcc actagcgagc
     9781 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tgttcgtgtg gatttactgg
     9841 tctacagcgc cggaattgca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9901 accgttcgct acaggtgaat ctggtgggtt atttcctgtg tgcgcgtgaa ttttcgcgtt
     9961 tgatgatccg cgatgggatt cagggacgca ttattcagat caactcgaaa tccggcaaag
    10021 tgggcagcaa acacaattct ggttatagcg ccgcgaaatt tggtggcgta gggctgactc
    10081 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
    10141 acctgctgaa atcgccgatg ttccagtcgc tgttgccaca atatgccacc aagctgggta
    10201 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10261 attatcagga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgta
    10321 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10381 gttaaggatc tccgtgagac taatagaatg cctgatgcgc tacgctcatc aggcatacag
    10441 gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcact gtcgccgtta
    10501 tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct
    10561 tcgacacact atgccagcaa gggcgggttg gcgtgggccg ttccagcggg cgctttaaac
    10621 cgcgggtcgt ggtcgccatc gccctggacg atcagcagcg catcgccgac accttgttta
    10681 tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggtatgcatg
    10741 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    10801 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    10861 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaagccg
    10921 ccattctgga gtatctgcaa aagcagggaa aatgctcggt tgaagaattg gcgcaatatt
    10981 ttgacaccac aggcacaact attcgcaaag atctggtcat tctggaacat gccggaaccg
    11041 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
    11101 ataaaacact catcaacacc cacaagaaag agctgatagc agaagctgcc gttggtttta
    11161 ttcatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag atggtgcctc
    11221 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgt
    11281 tatctgaact ggataatgaa caaactatcc tgatgccagg cggaacgttt cgtaaaaaat
    11341 cggcctcatt tcacgggcag ctggcagaga atgccttcga gcatttcacc ttcgacaaat
    11401 tattcatggg taccgacggc atcgatctca atgcgggcgt aaccaccttc aacgaggtct
    11461 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    11521 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctga
    11581 ttaccgacgc agatatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg
    11641 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
    11701 atgctggaat tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    11761 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    11821 ggccacattg gtaagaaaat cgccgcaacg cttgccagta ccggcacacc ggcttttttt
    11881 gtccatccgg cagaagcgct gcatggcgat ctggggatga tcgaaagccg cgatgtgatg
    11941 ctgtttatct cttactccgg tggggcgaag gaactggatc tgattattcc gcgtctggaa
    12001 gacaaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    12061 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    12121 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    12181 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc ctgctggagc actgggcgct
    12241 cgcttgctga ataaagtgca tcatctgatg cgccgtgacg atgccatccc acaggtggcg
    12301 ttaaccgcca gcgtgatgga tgctatgctg gaactcagcc gcaccggtct ggggctggtg
    12361 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
    12421 tggctggttg gcggcggcgc actcaccacg ccagtcaatg aagcgatgac ggtcggcggc
    12481 accacgttgc aatcgcaaag tcgcgccatc gacgccaaag agatcctgat gaagcgcaaa
    12541 atcactgccg caccggtggt ggatgaaaac ggcaaactca ccggcgcaat aaacctgcag
    12601 gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc cagccgatgc
    12661 aggttggcga cgtcggtttc cagcatccgc gcgcaggcag cccagttgtg atgattttgt
    12721 gccagtgcct gacgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    12781 ttaacaacgg gcaccgccgc cacttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    12841 tgttgcgcct caagaatcac ttcatcgccg ctgcgggtgg ctctcgccag cactaccgcc
    12901 cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg ttgcagtaaa
    12961 tttcgcgccc cggcacttaa taccacgcgg gagagcccct gccgcaaacg acactgctcg
    13021 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    13081 agtggaaaca cactcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct
    13141 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
    13201 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13261 aacgacaact cgccgatctc atcgagaaac agcgtgccgt tatccgccat ttcaaacttc
    13321 ccactgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
    13381 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    13441 ggcgaggcct catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
    13501 atcaggacgt tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg
    13561 cctggcgaca gaccgatcat ctgcgtctgt ttcaccgctt caaatggagc ggcatcgccc
    13621 ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
    13681 gccagcgcgg caatcaggcg taactcttcg tcgctgaaaa catcgaactg atcgggctgc
    13741 atcccgtcga gcgtcaatgc gccgatcagg ttttgcccgg caaacagcgg cagaccaacg
    13801 caggcgtgaa ccttcagact ctcctgccct ggaatcaaac cgtcataggg atcgggcaat
    13861 tcgctgtctg cgggaaagcg caccacatca cccgcgcggg caatcgcttc cagccgtgga
    13921 tgaccttcca gcgcaaagcg tctaccgagt acatccttcg ccagcccgtc gatggcaagc
    13981 ggaataaact gccgcgagtc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    14041 cgcagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    14101 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    14161 ctatagatat tgtcatatcg accattggat tgatagt
//
LOCUS       DAZFKY010000120        14132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFKY010000120 DAZFKY010000000
VERSION     DAZFKY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..5447
                     /locus_tag="ACHKCL_003224"
                     /pseudo
     CDS             19..5447
                     /locus_tag="ACHKCL_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5563..6627
                     /gene="hipA"
                     /locus_tag="ACHKCL_003225"
                     /pseudo
     CDS             5563..6627
                     /gene="hipA"
                     /locus_tag="ACHKCL_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            6898..7167
                     /locus_tag="ACHKCL_003226"
                     /pseudo
     CDS             6898..7167
                     /locus_tag="ACHKCL_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7601..8164
                     /gene="fimA"
                     /locus_tag="ACHKCL_003227"
     CDS             7601..8164
                     /gene="fimA"
                     /locus_tag="ACHKCL_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5764129.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            8525..9235
                     /locus_tag="ACHKCL_003228"
     CDS             8525..9235
                     /locus_tag="ACHKCL_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5764130.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9277..11928
                     /locus_tag="ACHKCL_003229"
     CDS             9277..11928
                     /locus_tag="ACHKCL_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5764131.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            11942..12472
                     /gene="ydeS"
                     /locus_tag="ACHKCL_003230"
     CDS             11942..12472
                     /gene="ydeS"
                     /locus_tag="ACHKCL_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764132.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12485..12988
                     /gene="ydeR"
                     /locus_tag="ACHKCL_003231"
     CDS             12485..12988
                     /gene="ydeR"
                     /locus_tag="ACHKCL_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764133.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13048..13962
                     /gene="ydeQ"
                     /locus_tag="ACHKCL_003232"
     CDS             13048..13962
                     /gene="ydeQ"
                     /locus_tag="ACHKCL_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764134.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3926 a         3006 c         3552 g         3648 t
ORIGIN      
        1 tgtatttgga gcgtttatat gaatagaatc tatcgcgtga tatggaattg cactctacag
       61 gtatttcagg cctgctcgga attaactcgc agggtaggta aaacatcaac ggttaatttg
      121 cgaaagtcct ctggactgac aacgaaattc agtagattga cgctgggtgt tttgctggca
      181 ctaagcggtt cagtgtctgg tgcgagtctg gaagttgata atggtcagat taccaatatt
      241 gatactgatg ttgcttatga tgcctacctg gttggctggt atggcactgg agtgcttaat
      301 attttggctg gcggtaatgc ctccttaacc actattacta ccagcgtcat tggcggtaat
      361 gaggattcgg agggtaccgt taatgttttg ggtggcacct ggcgattgta tgatagcgga
      421 aataatgcaa ggcctttaaa tgtgggtcaa tccggaacgg ggacgctgaa tattaaacag
      481 aagggtcacg tcgatggagg ctatttaaga ttaggtactc aagctgcagg cgtcgggacg
      541 gttaatgttg agggagagga ctcggttttg acgaccgaat tattcgaaat agggagttac
      601 ggtacgggct cattaaatat tacggataag ggttacgtca cgagttcaat agtcgccatt
      661 ttaggatatc aagcgaacag taatggtaag gttgtcgttg aaaagggtgg cgagtggcta
      721 ataaaaaata atgattcctc aattgaattt caaattggta atcaaggaac tggggaggcg
      781 actattcgcg agggtgggct gattacggct gaaaatacga ttatcggtgg caatgccacc
      841 ggtgtcggaa ccctgaatgt gcaggatcaa gactctgtca tcacggtacg cagactctat
      901 aatggatatt tcggtaatgg cgcagtcaat atttccaata atggactaat taataacaaa
      961 gaatattcat tggtgggcgt tcaggacggt tcccacggtg tcgtcaacgt gaccgataaa
     1021 gggcattgga atttcctcgg aacgggcgaa gctttccgct atatctatat cggtgatgct
     1081 ggcgacggtg aacttaatgt ctcgcgtgaa ggtaaagtag attcgggaat tatcactgcg
     1141 gggatgaaag aaacaggcac aggcaacctt actgttaagg ataagaactc cgttatcact
     1201 aatctcggaa ctaatcttgg ttatgacggc cacggcgaaa tgaatatcag taatgagggg
     1261 cttgttgtca gcaacggagg aagctctctc ggttatggag aaaccggcgt cgggaaggtt
     1321 agcatcacca cgggggggat atgggaggtc aataagaatg tctataccac cattggtgtt
     1381 gcgggcgtcg gaaacctcaa tattagcgat ggcggtaagt tcgtatcgca aaatattact
     1441 tttttgggcg ataaagcaag cggtatcggc acactgaacc tgatggatgc gacatcgtcg
     1501 ttcgatactg tgggtatcaa tgtcggtaat tttggtagcg gtatcgtaaa tgtcagtaat
     1561 ggtgccaccc ttaattcaac gggctatgga tttatcggag gaaatgcctc cggtaaggga
     1621 atagttaata tttcaacgga tagtctctgg aatttaaaga cgtcttctac taacgcccaa
     1681 ttgctacagg tcggtgtatt aggcacgggt gaactgaata ttaccaccgg aggtatagtt
     1741 aaagcgcgtg atacacagat agctctcaat gacaaaagta agggcgacgt gagggtggat
     1801 gggcagaact ctcttcttga aacattcaat atgtacgtag ggacatctgg tacgggtacg
     1861 ttaaccctga cgaatagcgg cacgctgaat gtcgaaggtg gagaagttta cttaggtgtt
     1921 tttgaacctg ctgtaggaac gctaaacatt ggtgcagctc acggtgaggc ggcggcagat
     1981 gccggattta tcaccaacgc gacgaaagta gagtttggct ctggcgaagg tgtttttgtc
     2041 tttaatcata ctaataacag tgatgccggc taccaggtcg atatgctgat tacaggtgac
     2101 gataaagacg gaaaagtgat ccatgatgca ggccatacgg tgttcaatgc agggaatact
     2161 tatagcggta aaacgctggt caatgacggc ctcctgacca ttgcgtctca tacggcagat
     2221 ggggtaacgg gcatggggtc gagtgaagta accattgcaa gccccggtac gctcgacatt
     2281 ctcgcatcaa cgaacagtgc aggagattac acgctgacca atgcgctcaa aggcgatggc
     2341 ttgatgcgag ttcagctgtc atcctccgac aagatgtttg gctttacaca tgcaacaggg
     2401 actgaattcg ccggtgttgc ccaactgaaa gacagtacct tcactctgga acgcgacaac
     2461 accgctgcgc ttactcacgc gatgttgcag tctgacattg aaaataccac atcggtaaac
     2521 gtgggagagc aatccattgg tggactggcc atgaatggcg gtacgctcat tttcgatacg
     2581 gatattcctg ctgcgacgct tgcagaggga tatatcagcg tcgatacgct ggttgtcggc
     2641 gcgagtgact acacctggaa aggccgtaac tatcaggtaa acgggacggg cgacgtgctt
     2701 atcggcgtgc ctaaaccgtg gaatgatcct atggcgaata accctctgac gacgctcaat
     2761 ttgctggaac acgatgataa ccatgtcggc gttcaactgg tgaaggcgca aacggttatt
     2821 gggtcgggtg gctcattaac gttacgtgat ttacagggcg acgaggtgga agcggacaaa
     2881 acgttacaca ttgcgcaaaa cggaacggtg gtcgccgagg gtgattatgg attccgcctc
     2941 acgaccgcac caggtgatgg tttgtacgtt aactatgggc tgaaagcgct gaacatccat
     3001 ggagggcaaa agctgacgtt agccgaacat ggcggagcct atggcgcaac ggccgatatg
     3061 tcggcaaaaa tcggtggtga aggggatctg gcaatcaata cggtgcgaca ggtttcgctt
     3121 tccaacggtc agaacgacta tcagggggca acctacgttc agatggggac attacgtacc
     3181 gatgcggatg gcgcgctggg caacacccgg gaactgaaca tcagcaacgc agccatcgtc
     3241 gatcttaatg gatcgacgca gacggtagag acattcaccg ggcagatggg ttcgactgtt
     3301 ttgttcaaag aagggtcgct gacggtaaat aaaggtggga tcagtcaggg tgaactgaca
     3361 ggtggcggaa acctgaatgt tacaggggga acgctggctg tcgaggggct taatgcacgc
     3421 tacaatgcgt taaccagcgt tagcccaaat gcggaagtca gcctcgataa tacgcagggg
     3481 ttaggcagag gaaatattgc caatgacggt ctgttaacgc taaaaaacgt gactggcgaa
     3541 ctgcgtaata gcataagcgg gaagggtatc gtgagcgcaa ccgccaggac agatgtagag
     3601 ctggatggcg ataatagccg ctttgtgggg caattcaaca ttgatacagg cagcgcgctc
     3661 agcgtcaacg agcagaaaaa cctgggtgat gcttccgtta tcaataatgg cctgctcacc
     3721 atctccactg agcgtagctg ggcgatgacg cacagtatca gcggtagcgg tgatttgaca
     3781 aaactgggta ccgggatcct gactcttaac aacgattcct cggcgtatca gggtacgacg
     3841 gatatcgtgg ggggggaaat tgctttcggt tccgactctg ccattaatac ggcaagtcaa
     3901 cacattaata tccataacag cggtgtgatg tcgggaaatg tcaccactgc aggtgatgtg
     3961 aacgttatgt ctggggggac actgcgtgtc gctaaaacca caatcggcga atcggcggca
     4021 acctggagaa tggcggcacg gttcaaatga acagtgaagg aggaaaaccg gggaatgtac
     4081 tgaccgttaa cggcaactat accggaaaca atggcctgat gacgttcaac gcgacgctgg
     4141 gcggcgataa ttcacccacc gataagatga acgtgaaagg cgatacccaa gggaacactc
     4201 gcgttcgggt tgataacatt ggcggcgtcg gtgcgcaaac ggtcaacggt attgaactca
     4261 ttgaggttgg cggtaattct gcaggtaatt tcgcgctgac caccggaact gtcgaagctg
     4321 gggcttacgt ctacacgctg gctaaaggga aggggaatga cgagaaaaac tggtatctga
     4381 ccagtaaatg ggacggcgta acgccagcgg atacacccga tcccatcaat aatccccctg
     4441 ttgtggatcc ggaaggccca tcagtttatc gcccggaggc cggaagctat atcagcaaca
     4501 ttgccgcagc caactcgctg tttagccatc gcttacacga ccgtctgggt gagccgcaat
     4561 atacagattc actgcattct caggattcag caagcagtat gtggatgcgt catgtcgggg
     4621 ggcacgaacg ttccagtgcc ggagacggcc agctaaatac tcaggctaac cgctatgtat
     4681 tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc gcaggatcgc tggcatcttg
     4741 gcgtgatggc aggctacgcc aatcagcaca gtaatactca gagtaatcgt gtgggttata
     4801 aatcggatgg gcgcatcagc ggttacagcg ctgggctgta cgcgacctgg tatcagaacg
     4861 atgcgaataa gaccggcgct tatgttgaca gctgggcgct gtataactgg tttgataaca
     4921 gcgtcagttc cgataaccgt tctgctgacg actatgattc tcgcggtgtg acggcctctg
     4981 ttgagggtgg gtataccttt gaagcgggaa catgtagcgg cagcgaaggg acgctgaata
     5041 cctggtacgt ccagccacag gcgcaaatca cctggatggg tgtgaaagat tctgaccatg
     5101 cccggaaaga cggaacgcgc attgaaacgg aaggcgacgg aaacgtgcaa acgcgacttg
     5161 gggtgaaaac ctacctgaat agccatcacc agcgtgacga tggtaaacag cgtgagttcc
     5221 agccttacat tgaagcgaac tggatcaaca atagcaaagt ctacgccgtg aagatgaatg
     5281 gtcaaaccgt aagccgtgat ggtgcgcgaa atctcggtga agtacgtacc ggggttgagg
     5341 cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta ggtgataaag
     5401 gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaaca gtataagccg
     5461 catgtcgaga tggcatgcgg cttaatattg ccaacttcaa aaggctttta aagcctccct
     5521 tttatccgcg atcgcggata tcgcagcgtt tatcccgtac agtttgattt attgtcagaa
     5581 atagggcgag acagcgttgg tgccgtgacg ttaatacccg aagacgaaac cgtaacgcgt
     5641 ccgataatgg catgggaaaa gcttactgaa gtcagacttg aagaagtatt aacggcttat
     5701 aaagcagata tcccgctagg catgattaga gaagaaaatg actttcgcat ctcggttgct
     5761 ggcgcacagg agaagacagc actgctcaga ataggcaatg actggtgcat tccgaaagga
     5821 ataacgccga cgacgcacat cattaaatta ccgattgggg aaatcaggca gcccaatgcg
     5881 acgctcgatc tcagccaaag cgttgataat gagtattact gtctgctgct ggcgaaagaa
     5941 cttgggttga atgtcccgga cgcagaaatc attaaagcgg gaaatgtgcg cgcgttagcg
     6001 gtcgaacgtt ttgacaggcg ttggaatgct gagcgaacgg ttttacttcg cttgccacag
     6061 gaggatatgt gtcagacatt cggtttacct tcatcggtga aatatgaatc agatggaggc
     6121 ccaggcatcg cgcggatcat ggcttttttg atggggttca gcgaggcgct tcgcgatcgt
     6181 tatgatttta tgaaattcca ggtcttccag tggttgattg gcgcaacgga tggccatgca
     6241 aaaaacttct ccgtatttat tcaggctggc ggcagttatc gactcacgcc attttacgac
     6301 atcatttcag catttccggt ccttggcggt acgggaatac acatcagcga tctcaaactg
     6361 gcaatggggc ttaacgcatc caaaggcaaa aaaacggcaa tcgataaaat ttatccgcga
     6421 cattttttgg cgacagcaaa ggtgctgaga ttcccggaag tgcagatgca tgaaatcctg
     6481 agtgactttg ccagaatgat tccggcagca ctggataacg tgaagacttc attaccgaca
     6541 gattttccag agaacgtggt gacggcagtt gaaaccaatg tgttgaggtt gcacggtcgg
     6601 ttaagccgag aatacggtat taagtaagag taagatatgg ggtttggtcg ttgttaatga
     6661 gcaggacaat catgaccgcc aacgactaga ttctcccttg gtttatattg gtgcgcataa
     6721 tgtatattat gttaaatcgt atctgagtga tacgaagtga ttttccggta gtcgttattc
     6781 gttcaggatt ccagaactta agcttctcct ctttcataat tggcctgtgc atgacggata
     6841 taggctggct ggtatgccga tttgccgttt gccgcatctt cccagcgttg aagactcaag
     6901 ctggcaaacc gcgtgcaaat gagcacgccc tctttccatc aacacttcag gcagcttgcg
     6961 ggaatgagtc cactccggta tcagaaatgg ctacgtctca atgaagtcag gcgaacgatg
     7021 ctgaatgaac attacgatgt caccacagca gcctatgctg tcggttacga aagcttatcc
     7081 catttccgac gggaatattt gcggatgttt ggagagtcac ctaagagaga tattaccagg
     7141 ttaagaaagt ctgtcggtca gctttaataa accctgcact tatctgttta gagcaattcg
     7201 gtgttagtta caccaggcaa acattaacta tagctaatga tttatagcca tataaaccat
     7261 tagtagccct tgacctttcc tgacacttcc cttgttcgca ataatattct gtttagtcac
     7321 ggtttataaa taacatgcat aatgaaatcg tcatgttatt tagcgacata aaatgttttt
     7381 gcaaatactc tcttcaacaa gtgcagcaat aaacttgttg tgattaggtg tccttgtgat
     7441 ttaaagatat tatcgttctt cgtgaaatgt aataaccctg gaaactatat tctcagggca
     7501 acaaccaatc aagaatgatt cttgttttat aagcaataac agaatgactt aattgttaac
     7561 tctctttatt cagctactta aatataaatt ttggagaatt atgaaactca aacatgttgg
     7621 tatgattgtc gtttctgtgt tggcgatgtc gtctgctgcg gtaagcgcag ccgagggtga
     7681 tgaatcagta acgaccactg ttaatggcgg tgttattcat tttaaaggtg aagtggtaaa
     7741 tgccgcttgt gcgattgatt ccgaatcaat gaaccaaacg gttgagctgg gtcaggttcg
     7801 ttcttctcgc ctggctaaag cgggtgacct cagctccgcc gttggcttca atatcaagct
     7861 gaatgattgt gataccaatg tttccagtaa tgcagctgtt gcattcctgg gtactactgt
     7921 caccagtaat gacgatacgt tagcgctgca gagttcagcg gcaggctctg cccaaaatgt
     7981 cggtattcaa attttggacc gtacgggtga ggtattaata cttgatgggg ccacttttag
     8041 tgctaaaacc gacttgattg atggcacgaa tatactacca ttccaggctc gttatattgc
     8101 tctcgggcag tccgtagctg gtactgcaaa cgcagatgcg accttcaaag ttcaatatct
     8161 ataattcaat attaaggtgg ctatacggca tctgaaatta aaacagggtg tgtgtggcat
     8221 ggctacgcac acccgagcaa ttcaccaatg tgaatctcat tattaatgat gagtggtttg
     8281 tatttggagc gctatgaaaa atcacataat atctaaacgt tcaataaaca acagtagtta
     8341 aacttactgt tattaaaaat atttactttt tagcaaggtt ttcctattaa aagagaacca
     8401 acctttatta caaacgcatt aagtctggaa acccggtgac tcgccaatgt attagcaaca
     8461 tttaccgatt gtctgatgta acggttttcc cgcttatcct cttaacacat ggagaatgca
     8521 tcagatgcaa actacgcgta ccccctactc tatttccttt atggcgacag tgttactttt
     8581 attgctcttt gcttgccata gcaccgttgc caacgcagct gtcgcactgg gtgcgacgcg
     8641 ggtaatttat ccggcaaacc agaaacaggt tttattgcct gtcacgaata atgatcctgc
     8701 cagtgtctat ctcattcagt catggataga aaatgctggt gaccaaaaag acacccagtt
     8761 tgtcattacc ccacctcttt tttccatgca aggtaaaaaa gagaatacgc tgcggataat
     8821 caacgccact aatcatcagt tacccggcga ccgtgaaagc ctgttctggg ttaatgttaa
     8881 agctattcct gctatggaaa aagaccagaa gaatgagaat acattacagc tggcaattat
     8941 tagccgcata aaaatgtttt atcggcccac gaacctggca atggcacctg aagaagcgcc
     9001 tgcaatgctt cggtttcgtc gcagcggtag caatctcact cttattaatc caacgcccta
     9061 cttcattacc gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca ccatggtgcc
     9121 gccaaaaggt gaagtgtcgg tggatatccc gcatgctgcc accggggata tcagttttca
     9181 gacgatcaat gattacggcg cattaactcc gcgtattaag gcaaccatgc agtaagccag
     9241 tgacctaatt aataataaac tcaatcggaa ttaataatga cagcctttcg tgccgcattt
     9301 aaggcatatc gtatgcatca ggtgttacta ctgcctcgct ttgcacggct taccattgcc
     9361 cttagcttag caactgcggt atttcccgtt gacgcagagt attattttaa tccacggttt
     9421 ttaagcaatg atcttgctga atctgtcgat ttatccgcct ttactaaggg acgcgaggca
     9481 ccacctggta catatcgggt cgacatttat ttgaacgacg aatttatgac tagtcgggat
     9541 attactttta ttacagatga taataacgca gatctcatcc catgcctgag tacagatctt
     9601 cttgtcagtc ttggcataaa aaaatctgcg ttattagaca ataaggaaca ttcagcagaa
     9661 aaacatgtgc cggacaacag cgcatgcaca ccacttcagg atcgtttggt cgacgcatca
     9721 actgagtttg atgttggtca gcaacatctg tcactctccg ttccgcaaat ttatgttggc
     9781 agaatggctc gcggctatgt ttctcctgat ctgtgggaag aaggaataaa tgctgggctt
     9841 ctaaactata gttttaatgg taattctatt aataatcgta gtaaccataa tgcaggaaaa
     9901 tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg gcgactacgc
     9961 gataactcaa cgtggagtta taacagtggg agtagcaatt catctgacag caataaatgg
    10021 cagcatatca atacgtcggc tgaacgtgac attattccct tacgctcacg gttaacggta
    10081 ggtgatagtt ataccgatgg cgatattttt gatagtgtga actttcgtgg cctcaaaata
    10141 aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt gattcatggt
    10201 attgcccgtg gcaccgcaca agtgagtgta aaacagaacg gatacgatgt ttatcagact
    10261 actgttccac ccggcccttt tactattgat gatatcaact ctgcggccaa tggtggtgat
    10321 ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt tccttattcg
    10381 tctgttccgg ttctccaacg tgctggatat acgcgttatg cgcttgccat gggggaatat
    10441 cgtagtggaa ataacctgca aagctccccc aggttcatac aaggtagctt gatgcatgga
    10501 ctggaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta tcaggccttc
    10561 aaccttggta ttggtaaaga tttaggactt tttggtgcct tttctttcga tatcacgcag
    10621 gccaatacga cacttgcaga tggcacccgt cacagcgggc aatcggttaa atccgtctac
    10681 agcaaatcct tctaccagac aggaaccaat atccaggtcg caggatatcg ctattctacg
    10741 caaggttttt ataacttatc cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag
    10801 cctcctaccg gagacagcaa tgagcagaca caatttattg attattttaa tctgttctac
    10861 agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggaaatta cggtgcgaca
    10921 tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca gcaaatatca
    10981 tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag ctattccaat
    11041 aatatatggc aaaacgatcg ggatcattta ctcgctttta cgcttaatgt tcccttcagt
    11101 cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag ttacagtatg
    11161 tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg tttatggcac tctgctgccg
    11221 gataataacc tgaattatag cgttcaggtc ggtaacaccc acggaggtaa tacatcgtct
    11281 ggcaccagtg gttacagtac tcttaattat cgtggagctt acggcaatac taatgtcggt
    11341 tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg gattattgct
    11401 catgctgatg gcatcacctt tggacagccg ctgggcgaca caatggttct ggttaaggct
    11461 cctggcgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga ctggcgtggc
    11521 tatgccatat taccatttgc gacagaatat agagaaaatc gtgtcgctct taacgcgaat
    11581 tcccttgcag ataatgttga actggatgaa accgtggtca ctgtcatccc aactcacggt
    11641 gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat gacgttgaag
    11701 tacggtaata aaagcgttcc attcggtgca attgtcactc acggagagaa taaaaatggc
    11761 agcattgtcg cggaaaacgg tcaggtttat ctgactggac ttccacagtc agggaaatta
    11821 caggtttcat ggggcaatga taaaaactca aactgtattg tcgattacaa gcttcctgaa
    11881 gtctctcctg gaaccttgct gaaccagcag acagcaatct gtcgctaagg aacaatagat
    11941 tatgaaatac aataacatta ttttcctcgg tttatgtctg gggttaacca cctattctgc
    12001 tttatccgca gatagcgtta ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt
    12061 ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc
    12121 aacgaccggt agcacaagtc cagccgtccc ttttcagatt acgttaagtg aatgcagcaa
    12181 agggacaacg ggggttcggg ttgcatttaa cggtattgag gacgcagaaa ataatactct
    12241 gttgaaactg gatgagggaa gcaatacggc ctccggttta ggtatagaaa tactggacgg
    12301 aaatatgcgt ccggtgaaac tgaatgacct tcatgccggg atgcagtgga tcccactggt
    12361 accagaacag aacaatattt tgccttactc cgctcgtctg aagtcaactc agaagtccgt
    12421 caatccggga ctggtgaggg cttcggcaac ctttaccctt gaatttcaat aatcagagtc
    12481 agatatgaaa agacttcaca agaggttcct cttagctacg ttttgcgcgt tattaacagc
    12541 aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg ctaaaccctg
    12601 cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacgc gcaatctgca
    12661 acaacctggt tctgcatctg gctggcacaa tattactttg tcattaaccg attgtccggc
    12721 tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg gttattacaa
    12781 aaatgaaggt actgccgaaa atattcagat agagcttagg gatgaccagg atgcgacgtt
    12841 aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg cacagtttcc
    12901 acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct
    12961 aatcaatgtg atctacacct ggcaataacg tcgattcgat tttcttagcc ttctttttaa
    13021 atcaattttt taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc
    13081 ggtatttatc ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc
    13141 gggtcaagta ttggagctgg tacaacctct gtttatgtta atctcgaccc tgtaatacag
    13201 ccgggccaga atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc
    13261 ggctggtacg acactgatca tataaacctg gtacaaggct cagctttcgc aggctctcta
    13321 cagtcatata aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca
    13381 aataccaacg tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat
    13441 attaccccag tcggggcggc cggtggagtt gtcataaaag ccggggaagt tattgcccgt
    13501 attcatatgt ataaaattgc cacgttagga agcggaaatc cgcgtaattt tacgtggaat
    13561 attatttcaa ataacagtgt tgttatgcca acaggcggct gcactgttga tagtcgtaat
    13621 gtcaccgtga atctcccgga ctttcctgga agcgcagaaa tcccccttgg cgtatattgc
    13681 tcaagcgaac aaaagctctc attttatctt tcgggtacta ccactgacag tgctagacaa
    13741 gtgtttgcta acacagcacc agatgcaaca aaagcatcgg gagtgggtgt ctctctgatg
    13801 cgtaatggaa aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc
    13861 aaagtacctc tgggtttaag cgcaacctat ggacaaacag ggaacaaagt ttcagccgga
    13921 accgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag
    13981 tctatgcgct ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta
    14041 cacttgtaag aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat
    14101 atgttaattt aaacactatg agagatgatg ct
//
LOCUS       DAZFKY010000121        13989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFKY010000121 DAZFKY010000000
VERSION     DAZFKY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..797
                     /gene="artJ"
                     /locus_tag="ACHKCL_003233"
     CDS             66..797
                     /gene="artJ"
                     /locus_tag="ACHKCL_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5764135.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(848..2329)
                     /locus_tag="ACHKCL_003234"
     CDS             complement(848..2329)
                     /locus_tag="ACHKCL_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO5764136.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GENYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2341..2661)
                     /locus_tag="ACHKCL_003235"
     CDS             complement(2341..2661)
                     /locus_tag="ACHKCL_003235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5764137.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(2683..4026)
                     /locus_tag="ACHKCL_003236"
     CDS             complement(2683..4026)
                     /locus_tag="ACHKCL_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5764138.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(4306..5433)
                     /gene="rlmC"
                     /locus_tag="ACHKCL_003237"
     CDS             complement(4306..5433)
                     /gene="rlmC"
                     /locus_tag="ACHKCL_003237"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5764139.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(5474..5962)
                     /locus_tag="ACHKCL_003238"
     CDS             complement(5474..5962)
                     /locus_tag="ACHKCL_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5764140.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6022..6867)
                     /gene="potI"
                     /locus_tag="ACHKCL_003239"
     CDS             complement(6022..6867)
                     /gene="potI"
                     /locus_tag="ACHKCL_003239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5764141.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            complement(6864..7817)
                     /gene="potH"
                     /locus_tag="ACHKCL_003240"
     CDS             complement(6864..7817)
                     /gene="potH"
                     /locus_tag="ACHKCL_003240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5764142.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(7827..8960)
                     /gene="potG"
                     /locus_tag="ACHKCL_003241"
     CDS             complement(7827..8960)
                     /gene="potG"
                     /locus_tag="ACHKCL_003241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5764143.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9055..10167)
                     /gene="potF"
                     /locus_tag="ACHKCL_003242"
     CDS             complement(9055..10167)
                     /gene="potF"
                     /locus_tag="ACHKCL_003242"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5764144.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            complement(10518..10994)
                     /locus_tag="ACHKCL_003243"
     CDS             complement(10518..10994)
                     /locus_tag="ACHKCL_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5764145.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11082..11984)
                     /gene="rimK"
                     /locus_tag="ACHKCL_003244"
     CDS             complement(11082..11984)
                     /gene="rimK"
                     /locus_tag="ACHKCL_003244"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5764146.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12045..12767)
                     /gene="nfsA"
                     /locus_tag="ACHKCL_003245"
     CDS             complement(12045..12767)
                     /gene="nfsA"
                     /locus_tag="ACHKCL_003245"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5764147.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(12751..13038)
                     /locus_tag="ACHKCL_003246"
     CDS             complement(12751..13038)
                     /locus_tag="ACHKCL_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5764148.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13198..13455
                     /gene="grxA"
                     /locus_tag="ACHKCL_003247"
     CDS             13198..13455
                     /gene="grxA"
                     /locus_tag="ACHKCL_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5764149.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(13485..13862)
                     /locus_tag="ACHKCL_003248"
     CDS             complement(13485..13862)
                     /locus_tag="ACHKCL_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5764150.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         3751 a         3750 c         3269 g         3219 t
ORIGIN      
        1 ggcattgtta tcccatgccg cagacacggc caataatcat aagattgaca gacgggagtt
       61 ccatcatgaa aaagttagtt cttgccgcat tactggcatc ctgtgcgttc ggcgcttctg
      121 ccgcagagaa aatcaatttt ggcgtatcag cgacctatcc gccgttcgag tcgatggatg
      181 ccaataacca gatcgtcggc tttgatatcg acctagcaaa agcgttatgc aaacagatgc
      241 aggcagaatg tacttttacc aatcacgcgt tcgacagcct gatcccgtca ctgaaattca
      301 ggaaatatga cgccgtaatc tccggtatgg atatcacacc agatcgtagc aagcaggtat
      361 cgttcaccac gccctattac gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca
      421 aaacgtttgc cgacctgaaa ggcaaacgca ttgggatgga aaacggtact acgcaccaga
      481 aatatattca ggatcagcac ccggaagtga aaacggtctc ctatgacagt tatcaaaatg
      541 cctttatcga tctgaaaaat agccgtattg atggcgtatt tggtgacact gcggtggtta
      601 atgaatggct gaaaaccaat ccgcaactgg gcgttgctac tgaaaaagtg accgatccgc
      661 aatactttgg caccggtctg ggtatcgctg tacgtccgga taacaaagct ctgctggaaa
      721 aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcaacaa atcagcgatc
      781 agtggttccc gcaataatta ttcatttgcc ggagtgggcg aacacccact ccggcaaaat
      841 cacttctcta cgctaatccc aggcgctgaa actgctctgg cggcgcatca ttatctttca
      901 tcaactgaat cagcaacgcg atcatccggg actcgatcac atcatcatga atcggatgta
      961 gctgctgcgg atcgttttgc agatcaaaca gcaaatgacc aaactgattc aaccccttat
     1021 atttttcctg tgccgggatg cgtaatacct gtaacccttt ggtaaaacga aacggtgggg
     1081 ccagcgataa agcccgcatc tcatccaccg gatagcggct gttcattttg caggacatca
     1141 acgtgtagtt ggcaatatca tcctcacgcc ccggcagcgc tgcacgcata tacacataac
     1201 gcccatcagt tacgttagca tgaccactga aaacaccaaa catcgccgag ctgcgtactg
     1261 gttcatcgtg actgaccgtt ttcgccagcg gttgcccctg cacatcgggc ggcactggct
     1321 gttgaaaaaa gtcatacaac gttggcgccc agtcgatcat ttgtaccagt gcctgtcgcc
     1381 gctctccttt tacaccactg cgcgggttcc agataaacaa cggattattg gcaacttcgt
     1441 tgtaataagg catctggttt ttcgcccacc agccatgctc accgagtaaa aagccgtggt
     1501 cagtgccgac aatcagcatg gtatctcgcc acagatcgtg ctcatccatc aggtcgagaa
     1561 tgcgccccag gttgcgatcg cacatcgaca ccagcgaacg ataccggcaa cggatgtgct
     1621 ccacggcctc gtcgctttcc tgcacccggc cacgcggcca gtcataatgc ggtccatccc
     1681 agttatcttc atacaaggaa agatactctt ccgttgtgtg aaacggctcg tgcggatcaa
     1741 acgtttcgat ctgcaataac cagttatctt ccgcgtgatt tttgtggata aaggcacagc
     1801 ctgcatcaaa cactttagtt tgtggaaaat ccgcttcctg ctgaatatat tcccggtttg
     1861 cccagtcatg gcgccacaga ccagaaacac cgccaccggt ctgcttttgc ggcacccgta
     1921 gcacctcggg aataggcggt tgcgcaacgc ttgcatgcca gtgatcgcct tcctgtccgc
     1981 gcacaatctc ccacgaactg tagcgattat gataattctc gccgccatct tcccagtaat
     2041 gcaaatgatc gcttatcagg tgggtataga tccccgcctt tttaagcagc tccggcatgg
     2101 aatcgtcaaa cggttctaac ggcccccact cccgatgcag gaagttacat cgcccggtat
     2161 gcaactcccg ccgggcaggc atacacggca tactgccgac gtaactgttt tcaaaggtgg
     2221 cggcatgagc cgccagacgt tggaaattag gcgctttggt taacgcatcg ccataaggtg
     2281 gcagataacg tttattcaga ctatcgaaca gcagaataat cgctctcatt acgccctacc
     2341 ttattgatta attaaatgcg gaaaatgttg ctgaaccact tcataaattt gctgaccaat
     2401 agtttcaccg tccataatat tctctatcgg aatcaaatat tgcccttgtt tcgggtcaag
     2461 actaaccaca tcgagtaatt ttttggaaat agcaaaaata tccgcccagt cggtaaatcc
     2521 gccgacttca cctgcggcgg tatgttccat ttcaactttg aaatcatatt tggtcagcgc
     2581 ttttttaacg accgtttgta acataacgct actgcctaca ccgtaaccac agacaaacag
     2641 aatattaatt gtatctttca tttttaaacc cttaatattt aattaacccg cgtaaggacc
     2701 aagatggaat gttgcagcga taaatttcag aacttcacaa atagcaggcc atacggttgc
     2761 ccagtcaaat attcctgtcc agccgacgcc attttcagca agcccggtta acggaatagc
     2821 ccacaccgta ccgaaggtct gaataatacc cagcaagaac gtacagataa ttacggaacg
     2881 gtatccgccc atgcggtttg ctaatacgcc aattggaccg ccagagaaaa acagaggtac
     2941 aaagccaggc aataccataa ttgggctgtg aaccagtaat aagatcccca tcgaaaaaat
     3001 ggtgccaaaa gtacagaaga cgaagcctaa cgttgcggca ttgggtgaaa aaggtaacag
     3061 cgctgcaaca tcgacagcag gaatagcgtt aggaataaag cgatcttgcc agcctttaaa
     3121 cgaaccgtta atttcgccca ccatcatgcg cacaccctgc agcagaatga ccatatacat
     3181 ggagaactgt aagccaaggt tgatgatata gatgtaccag ggcttgcctt tcgccattaa
     3241 ctggacatta tcgatacccg ttgatagcaa gaaaccaccg acaaacagcg tcatcacaat
     3301 ggcgatagcc gttacgttat ggttaaaaat cgccagccac ccgggaagtt tcaggttttc
     3361 agcatcgtgc ttttcagggt caccaaattt atgggcgaat ttggagaaaa accacaatcc
     3421 caacatctgg ttatgagcaa tggtaaaacc ggcatctttg gtaactttgg caatgggctt
     3481 aacaatgagc gtggtggaga acgcccagaa tacacccgtt aatattccgg caataataat
     3541 ggatgtagtc cagctaaaac ccagccaaaa gacaatcaac cataataccg cctgagaatg
     3601 tgaaacaccg gtattccctg tcaaaaatat ccctttggca ccagtatacc gaccaaataa
     3661 taccagcagc aggttggtaa aaaacgccag tacaaataca tacccgacat aaccaaaatt
     3721 atcccccaat gcaatttgcg ttgccgcttt cattgcgtag gaatccatta aatatccgtg
     3781 aacaccatag gctttactta cggcttctgc taccggttta aatgttgcgg aaaactggaa
     3841 accaccaaat aacaccatca ttaatcccac catggcgctg accatgccgg taataatttt
     3901 tattggcgat ttcttatcaa gggcataacc aatacccacc agcaaaccaa ttaaaattgc
     3961 cggttcagtt aaaaacgttt gaataacatc tatgaatttg gcaaacatcg tcggaacgcc
     4021 ctccatacgc gtacatttcc gttgtcaatg catgaacctt ctgctgacgt taataatgtc
     4081 tcaggaaatt ttgtgtttcg ctaagcaatg cctctgtatg tattcggtaa gtgcactgta
     4141 gtaatcaaaa cagttcaatt aaagatctta tttgattatt tgtgatttga tttgcaaaaa
     4201 tagagcaata tataaccgtg atcgatcggt tattattaat cgatcgatca cgattattat
     4261 tttggatgga tgtatgccga ggtgatattg atttgaatga tgtatttaca tttttaccag
     4321 cagcgtcagc acttcatagt gcgcggtgtg cgggaacata tcaaaaagct gtacccgttc
     4381 gatgcggtaa cctggcagtt cgcggatatc tttcgccatg gtttgggcgt tacagctgga
     4441 gtagataata aaacgcggtg ccatcgttga gagataatca cacagcggtt taccaatgcc
     4501 gcggcgcggc gggttaacca gcaccagctc cggcacctcc ccctgagcgg tggcaaactg
     4561 agtggagtcc agcgcctgaa attgcagacg cgttaagccc agttcagcgg ctgactgctt
     4621 tgcacaggca atggcctcta gcgcaatttc gatcccggtt aactgcatgt caggcgtcgc
     4681 gcagtgtaaa ccaaagcccc ccacaccgca gaacagatcc cacatatgtt taaccggcag
     4741 ctgtcgtacc cagtcgcgcg cggtagcgta cagctggctg gcgaccgccg gattagtctg
     4801 gaagaaactt tgcggacgga tccacagcgg cacatcgtta aaacgctcta ccagcgcctg
     4861 ttgttcggtc aggtagatct ccgtctcccc ttccataatc gccatatgta ccggctgaat
     4921 attgacggtg ataactttta gctgcggcaa ttgtgcctgt aaccacggca atgccttacg
     4981 cagttgtgct agtttggttt cagaacgcag tacaaagcgc agcatcatgc cgccatcgct
     5041 ctggctttca gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca cgttgtaggg
     5101 cgttaacccc gctcgggcga taaacggttt tagcgccgca aaaacgggcg caaatgaggc
     5161 aggataaagc gggcagtcac aaaggtcttc cggtgtgccg tcgcgatgca gcataccgag
     5221 cagtggtttt tcaacgctac cactcaccac cattttggct ttattacgaa acccttgttc
     5281 cgggcctgac accggcgcgc accattcctc aaccggaaag tcggcgagca gatttttaag
     5341 atcggcggtt ttagcggaga gttgctctgg aatcggctgc gttatccact gacaggaacg
     5401 acagcgaccc gcgtcgtaaa gtgcgcactg catacattga ccttcacatc atcaggggcg
     5461 acgattatac acattattgc aactggaaga accgccgact ggtcgaggga acgaacagca
     5521 gcatcaggat gagcatatcc ggcagcttct gcagcatcag gctatggaag atttcacgtt
     5581 ttgattcacc gggaatgctg aacagctccg gataaccgta ccccagcgaa gccgcccaca
     5641 agtaacttgc ggcagtgatt tgtgtcagca gatacagcca gcgcgcccag cgacgccctt
     5701 tcaccagtga aaacgcacac cagatctcaa tgaaaaccag caccagactg cttaaaaaga
     5761 ccagcgttaa actccaggtt tgtacgctgc gatgaatgaa ctcgccaata ccgcgcacgc
     5821 ccagcgtatt gaaaatcatc agcacgtcga ggccacggat cataataatg gcgagcgccg
     5881 ccacctgcac aagcgcaggc acattcaggc gagcatgaga tgaagatgtt ttcttaaaaa
     5941 atcccaacgt ttcgtcttcc atgaaaacaa tgccgcgaca tacgcggcat tatgtagcca
     6001 ggttggcaaa ttttagtgtc ttcagccacg tcttgcacac tggatgtcgc gtatccgctg
     6061 tttttctgcg cgagccatca gataccaggc gataaatccg acaattccga ccgcgccgag
     6121 gatcaatgtt gctagggcgt tgatttccgg attcaccccc atccgcacgc tggaaaagac
     6181 cagcatcggt aacgtggtgg ctcccggccc ggatacaaag ctggcgatca ccagatcatc
     6241 aagcgacaaa gtaaaagcca gtaaccagcc agaaatgacc gcgggcatga tcatcggtag
     6301 cgtaatgacg aaaaacactt tcagcggcgt cgcaccgaga tccatcgctg cttcttctat
     6361 cgagcgatcc agttcccgaa gacgcgacga aataacgact gccacataag ccgtacagaa
     6421 agtgacatgc gccagccaga tggtaagcat accgcggtcc gcaggccagc caatggcatg
     6481 agcaagcgcg acgaataata acaacagcga caagcccgtg atgacatctg gcattaccag
     6541 cggcgcggtg atcataaagg caaagccatt tgatccgcga aacctgccaa agcgcaccag
     6601 caccaccgcc gcaatagttc cgaggatcgc cgccgccgtc gccgcacagg ccgcaattgt
     6661 cagacttaaa ccaaccgcac tcatcatcgc gtcatcgcgc aataattcgc cataccagcg
     6721 cgttgaccag ccggcccaca ccgtcaccag cttcgagctg ttaaacgaat agataaccag
     6781 catcagcatt ggcgcgtaga gaaaggtgaa gcccaacagc aaaatcacaa tccgccaggg
     6841 cgaacgaact accggtaaat tattcatccg tgttctccca cgcttttttg ctggtgtttg
     6901 tgaaaccaca ttatcggcac aattagcagc agcaacatga tgatcgctac cgccgaggcc
     6961 accggccagt cgcggttatt gaaaaactct tgccatagca cgcgcccgat catgatgctg
     7021 tccgggccac cgagcagttc cgggatcaca aactcgccca cagccgggat aaacaccagc
     7081 atcgatccgg caataatccc acctttagtc agcggcacga tcacagtaaa gaacgttttc
     7141 agcggtcgtg caccgagatc cagcgctgct tccaccagcg aataatcaat acgaatcaac
     7201 gcggtataaa tcggcagcac cataaacggc acgtaggcgt aaacaatgcc aatataaact
     7261 gccagattgg tatgaagaat ggtcagcggt tgatcgataa cccccagcca cagcagaaaa
     7321 ttattcagca caccgttgtt ttttaatatt cccatccagg cgtagacacg aatcagaaac
     7381 gaggtccacg acggcaggat caccagtagt aataagatat tacgggtcga aggcttactg
     7441 tgcgccaccg cccacgccag cggatagccg atcagtaaac agcaaaatgt cgaaatcgcc
     7501 gccacctgta acgactggag ataagcatcg aaatagagcg gatcgccggt cagttgcagg
     7561 aaattaccga aattaagggt gatggacagt tgcccgtcag cccactccat cagttcggta
     7621 taaggtggaa tagcgcgcgc catctccgcc aggcttattt taaagacaat cagaaatggc
     7681 agcagaaaca gcaagatcaa ccagatatac ggcaacgcaa tgaccagttt gcgcccatgc
     7741 ttcatttgca actgcgacaa ccacagctta aatccgcccg gcggtttcga ctgggcagca
     7801 ggttcaagtg tactcatctt tgctccttaa accgtcagca ccacacagct gtccacttcc
     7861 cagcacaaac gcacttcgtc gccccaggtc ggtaaccctt tacgatgacg atgggcgttt
     7921 tgtagctggg cgctaatcat ctgcccactt ttcagacgaa cgtgatacac cgaaagatcg
     7981 ccgagatagg caatgtgtat cacctccccc accgcgaagt tacaaccatt ggcgggcggc
     8041 tcttcgcaaa gcatgatttt ttccggacgc agcgctacgt gtaccggcac gttatcgacc
     8101 actgaagcat ccgcatcgac tttcagtgga tgcaccagcc ccggcgaata aagcaccaga
     8161 ccatcttcct gacgctcttt gagtacacct tcaaagacat taaccgagcc aataaattcg
     8221 gcgctatagc gggtagtcgg atgctcgtag atctcttccg gttcgccaat ctggacaaat
     8281 ttcccacgat tcataatggc gatacgcccc gccatggtca tcgcctcttc ctgatcgtgg
     8341 gtgaccatca cacaagtcac accgacgcgt tccagaatat ccaccacttc aagctgcatc
     8401 cggtcacgca gctttttatc cagcgcgccc atcggctcgt cgagcagtaa tagtttcggg
     8461 cgcttggcaa ggcttcgggc cagtgccaca cgctgtcgct gaccaccgga aagctgatgc
     8521 ggtttgcgtt tggcgaactc ctgcatatgc acaaggccga gcatctcatt gacccggctg
     8581 gcaatttccg ctttcggtag tttgtcctgt ttcaggccaa aagcgatgtt ctgttccacg
     8641 gtcatatgcg gaaacagcgc gtaagactga aacatcatat tgatggggcg caggtaaggc
     8701 ggcacctgtg acaaatcgac gccatcaagc ataatctgcc cggctgaagg ttgttcgaaa
     8761 cctgccagca tacgcagcag cgtggacttg ccacagccgg atgcgcccag cagcgcaaag
     8821 atttcacctt tgtagatggt caggctgaca tcatcaaccg catgctgacc atcgtaggat
     8881 ttggtcagat tgcggatttc taatagcggg gtcagcgcct tacgggtttt cgcctggggg
     8941 cgagggatag cgtcattcac ggctgctctc cggcattaaa tgtgtgcaag cgcaccatgt
     9001 tggtgcgaat tgccggaggg agtggcgctc ccgcccggca tctaaggctg cggattattt
     9061 tccgctcttc actttggtcc acgcgcgggt gcgcacacgg tcgattttcg gagcctgtac
     9121 tttcagagtg aacaacttcg cgcggacatc cgcaggcgga taaatacccg ggttatcacg
     9181 gacttccgca ctcaccagcg gcgtggctgc tttattggcg ttagcataga acacatggtc
     9241 agagatatgt gccaccacat caggacgcag caggtaattc aggaactgat aggcttcgtc
     9301 tttatttttg gcatccgcag gcatggcgaa tacatcaaag aacgccatcg ccccttcttt
     9361 tggaatcgag aacgagacat tcacgccatt cttcgcttcc ttcgcgcggt ttgacgcctg
     9421 ccagacatca cctgcccagc cgatagcgac gcaaatatcg ccgtttgcca ggtcgttaat
     9481 atattgagat gaatggaaat aacgaatgtt cgggcgcagc tttaacagca gatcggtagc
     9541 cggtccggtg taatcatccg ctttagtgct gttgggatct ttgccgagat aattcagcac
     9601 ggtagcaaaa acttcttctg gcgcatcgag gaaagataca ccgcagcttt tcagtttttc
     9661 cagattttca ggcttgagga tcaagtccca gctatcgacg ggcgcgtttt cgcccagcac
     9721 cgctttgact ttatcgacgt tatagccaat cccggtcgtc gcccacatat agggcatagc
     9781 aaatttattg tcgggatcgt gtttggcgac caacttcagc agttccgggt cgagattctt
     9841 ccactccggc aatttgcttt tatccagcgg ctggaaaact cccgctgtca gctggcgttc
     9901 aagaaagctt gctgacggca ccaccaggtc aaagccggtg ctcccggcca ttaatttgcc
     9961 ttccagtact tcgttagagt cgaaaacatc gtagacgact ttaataccgg tttctttttc
    10021 aaagttagcc accgtgtcgg gggcgatata atcagaccag ttataaatgt ggagtgtttt
    10081 ttgttcagcc gcgagcgtgc cgacagagac ggccatcaga gcacccgcaa ccagacccga
    10141 aagccatttt ttatttaagg cggtcatatc tctttccttc tgaaagttcg ttaacaaacg
    10201 aacaaaagtt gtcacactga aagatatgca ataaacgtgc atatttttta tcggcgcggt
    10261 gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc atcgcaagaa
    10321 taactttagc cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt
    10381 tatgcgtatt tgctctatgt gataaatcca aatcgacaca agagaagtga aataatcttt
    10441 aagaaaaggt taaaaacagc agataatttt gcagcgcgca gccactgcac cagcgactgc
    10501 gtgctcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca
    10561 tcgtcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc
    10621 tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat
    10681 aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc
    10741 acggtcagcg aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa
    10801 ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca
    10861 tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat
    10921 tcaaaaaaac tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg
    10981 accagcgatg tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc
    11041 aaaaagagca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg
    11101 caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata
    11161 ccggtggttt tttctattcc ttccagcccc ggcgacgcat tcacttccat caccaacggc
    11221 ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct
    11281 tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga
    11341 tgcaaattgg aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca
    11401 tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc
    11461 agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctgacgcgtc
    11521 tccgccagca cgacgccaat tccctgcgtg ccttcaacca acttgaccac cagcggcgca
    11581 ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca
    11641 ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg
    11701 gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc
    11761 gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt
    11821 aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa
    11881 agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta
    11941 cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc
    12001 agacctggtc aaaagcccgc gtatcataca tcggcgtggc ggttttagcg cgtcgcccaa
    12061 ccctgtttgt gcaaataatc cagaataaat gggcggcttt ctttaatgat tgttcggcgg
    12121 atatgatcgc tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattcagcc
    12181 agttgttcgt catactgcgc cagtgcgtct ttatccagcg gttgatagct gttttcatgc
    12241 accaaaatgg aggacggtaa acgcggctta agatccggat tatccgcagg ccagccaagg
    12301 cacagcccaa acagcggcag aacatgctgc ggtaatttca gcagtttcgt caccgcttca
    12361 atattattgc gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcggtt
    12421 aatgcattct gcgccatcat tgccgtatca acgacaccga gcagcaattg ttccgccagg
    12481 ccaagctgag catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag
    12541 aactccgccg cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcttcacgt
    12601 aacgctttgt cggtaatgcg aataatgcta ctgcactgca aaaaactgga actggacgtc
    12661 gcacgggcgc tgttaataat cgcctcacgc tgcgcctcgg aaatgggttc atcagtgaaa
    12721 tggcgaatgg agcgatggcc acaagtaagt tcaatggttg gcgtcattat ctttttctct
    12781 ttctgaacgt gaatactgcg gtggacggtt catcagctgt ggggcaagac gttttgccac
    12841 ctgaagaata accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag
    12901 aatctgcact tctggccgag aaaatggctc aggcagcgac agggagtcgc ttaccgacag
    12961 cagcgccacc gccagcaaca tcattccgat aaattccagt atcaacacgc ttttaggcaa
    13021 tttaccgatc gcgcgcatac gcttccctct gcaaagtgaa ccttcagtct aaaacttttc
    13081 actgtattgt gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt
    13141 tcagcgaata cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg
    13201 caaaccgtta tttttggtcg tccgggttgc ccttactgtg tgcgtgcaaa agatctggct
    13261 gagaaattga gcaatgaacg cgatgatttt cagtatcagt atgtagatat tcgtgcggaa
    13321 gggatcacta aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag
    13381 atttttgtcg atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagag
    13441 aatctggacg cctgatcgtc tgacaagccc tcgcgttgag ggctttactg attttttctg
    13501 tgctgtggtt taaacaagct actgataaat aagaaacaca gtgcccccag cgcacaccag
    13561 aacaccgcgc ttagtaacca tgccagctct tgccagaatg agcgggtcgg tgaaaaaaac
    13621 agccgcataa taagcattga acagggtgcc gccagcattg cgccaaatag aggtttcagg
    13681 acttctctac gctgtgaaaa gaaactggca actgctccag gaagaatgaa aaacagcaag
    13741 ccgatttcag gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaagg
    13801 cacaccacaa tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc
    13861 actcgttcct cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta
    13921 agataaagcc gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga
    13981 tacctaatg
//
LOCUS       DAZFKY010000122        13943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFKY010000122 DAZFKY010000000
VERSION     DAZFKY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            137..646
                     /gene="ybgA"
                     /locus_tag="ACHKCL_003249"
     CDS             137..646
                     /gene="ybgA"
                     /locus_tag="ACHKCL_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5764151.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHKCL_003250"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHKCL_003250"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5764152.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKCL_003251"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHKCL_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5764153.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKCL_003252"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHKCL_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5764154.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKCL_003253"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHKCL_003253"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5764155.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKCL_003254"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHKCL_003254"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5764156.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKCL_003255"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHKCL_003255"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5764157.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHKCL_003256"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHKCL_003256"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5764158.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHKCL_003257"
     CDS             complement(7859..8905)
                     /gene="abrB"
                     /locus_tag="ACHKCL_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5764159.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQPLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHKCL_003258"
     CDS             complement(9054..10115)
                     /gene="ybgO"
                     /locus_tag="ACHKCL_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764160.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHKCL_003259"
     CDS             complement(10112..10843)
                     /locus_tag="ACHKCL_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5764161.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDHE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHKCL_003260"
                     /pseudo
     CDS             complement(10858..13317)
                     /locus_tag="ACHKCL_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(13367..13933)
                     /locus_tag="ACHKCL_003261"
     CDS             complement(13367..13933)
                     /locus_tag="ACHKCL_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764162.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3379 a         3452 c         3796 g         3316 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg ggttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccatcgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gtgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtctagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatt tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tcc
//
LOCUS       DAZFKY010000123        13887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFKY010000123 DAZFKY010000000
VERSION     DAZFKY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            137..1246
                     /gene="livK"
                     /locus_tag="ACHKCL_003262"
     CDS             137..1246
                     /gene="livK"
                     /locus_tag="ACHKCL_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5764163.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1294..2220
                     /gene="livH"
                     /locus_tag="ACHKCL_003263"
     CDS             1294..2220
                     /gene="livH"
                     /locus_tag="ACHKCL_003263"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5764164.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2217..3494
                     /gene="livM"
                     /locus_tag="ACHKCL_003264"
     CDS             2217..3494
                     /gene="livM"
                     /locus_tag="ACHKCL_003264"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5764165.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3491..4258
                     /gene="livG"
                     /locus_tag="ACHKCL_003265"
     CDS             3491..4258
                     /gene="livG"
                     /locus_tag="ACHKCL_003265"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5764166.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4260..4973
                     /gene="livF"
                     /locus_tag="ACHKCL_003266"
     CDS             4260..4973
                     /gene="livF"
                     /locus_tag="ACHKCL_003266"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5764167.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5092..5838
                     /locus_tag="ACHKCL_003267"
     CDS             5092..5838
                     /locus_tag="ACHKCL_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5764168.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6198..7514
                     /gene="ugpB"
                     /locus_tag="ACHKCL_003268"
     CDS             6198..7514
                     /gene="ugpB"
                     /locus_tag="ACHKCL_003268"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5764169.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7612..8499
                     /gene="ugpA"
                     /locus_tag="ACHKCL_003269"
     CDS             7612..8499
                     /gene="ugpA"
                     /locus_tag="ACHKCL_003269"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5764170.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8496..9341
                     /gene="ugpE"
                     /locus_tag="ACHKCL_003270"
     CDS             8496..9341
                     /gene="ugpE"
                     /locus_tag="ACHKCL_003270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5764171.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9343..10413
                     /gene="ugpC"
                     /locus_tag="ACHKCL_003271"
     CDS             9343..10413
                     /gene="ugpC"
                     /locus_tag="ACHKCL_003271"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5764172.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10410..11153
                     /gene="ugpQ"
                     /locus_tag="ACHKCL_003272"
     CDS             10410..11153
                     /gene="ugpQ"
                     /locus_tag="ACHKCL_003272"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5764173.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11140..11580)
                     /locus_tag="ACHKCL_003273"
     CDS             complement(11140..11580)
                     /locus_tag="ACHKCL_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5764174.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11700..13445
                     /gene="ggt"
                     /locus_tag="ACHKCL_003274"
     CDS             11700..13445
                     /gene="ggt"
                     /locus_tag="ACHKCL_003274"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5764175.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         3114 a         3489 c         3945 g         3339 t
ORIGIN      
        1 atcgctatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa aacagtgcag
       61 tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac aacatcacga
      121 atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact
      181 ggcaatttca cacaccgctt tagccgacga tattaaagtt gccgttgtcg gcgcgatgtc
      241 cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga
      301 cattaatgcc aaagggggaa ttaaaggcga taagctggtt ggcgtagaat atgacgacgc
      361 ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg gcattaaata
      421 cgttattggt catctgtgtt cttcttctac ccagcctgcg tcagatatct atgaagacga
      481 aggtattctt atgatctcgc cgggagcgac aaacccggag ctgacccaac gcggttatca
      541 acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg caaaatacat
      601 tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac agtatggcga
      661 agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg tcgtcttctt
      721 cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga
      781 aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca
      841 ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtgg gtaatgcgtc
      901 gttgtcgaac attgccggtg atgccgccga aggcatgttg gtcactatgc caaaacgcta
      961 tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca agaaagatcc
     1021 gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcaa ctgcccttga
     1081 acgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa
     1141 caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg
     1201 tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgaaaat cccaccgccc
     1261 gtgaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt gtatttcttg
     1321 cagcagatgt ttaacggcgt cacgctgggc agtacttacg cgctgattgc catcggctac
     1381 accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt ttacatgatc
     1441 ggcagctacg tctcatttat gatcatcgcc gcactgatga tgatgggcat tgataccggc
     1501 tggctgctgg tagcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg
     1561 agtatcgaac gggtggctta ccgcccggtg cgtaactcta agcgcctgat tgcactcatc
     1621 tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgactga aggttcgcgc
     1681 gacgtggcgc tgcccagctt gtttaatggt cagtgggtag tgggtcatag cgaaaacttc
     1741 tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct cgccatgctg
     1801 gcgctgacga ttttcattcg ttattcccgt atggggcgcg cctgccgtgc ctgcgccgaa
     1861 gatctgaaaa tggcgagcct gcttggcatt aacaccgacc gggtgattgc tttgaccttt
     1921 gtgattggcg cggcgatggc ggcggtggcg ggcgtactgc tcggtcagtt ctacggcgtc
     1981 attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt
     2041 gggattggca gcattccggg agcgatgatt ggcgggctga ttctggggat tgcggaggcg
     2101 ctctcttctg cctacctgag tacggaatat aaagatgtgg tctcattcgc cctgctgatt
     2161 ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga
     2221 aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc ctggcgggcg
     2281 tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag
     2341 acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc cagcttttgc
     2401 gaccggcttt ccagaaaggg ttgaaaagcg tttccggacc gaagtttatt ctgcccgcca
     2461 ttgatggctc cacggtgaag caaaagctgt ttctcgtggc gctgctggtg cttgcggtgg
     2521 cgtggccgtt tatggtctca cgcgggacgg tggatatcgc caccctgacc atgatctaca
     2581 ttatcctcgg actcgggctg aacgtggtgg tagggctttc tggtctgctg gtgctggggt
     2641 acggcggctt ttacgccatc ggcgcttata cctttgcgct gctcaatcac tattacggct
     2701 tgggcttctg gacctgtctg ccgatagccg ggttgatggc ggcggcggcg ggcttcctgc
     2761 tcggcttccc ggtgcttcgc ttgcgcggtg actatctggc gatcgttacc ctcggtttcg
     2821 gcgaaattgt gcgcatattg ctactcaata acaccgaaat taccggcggt ccgaacggta
     2881 tcagtcagat cccgaaaccg acttttttcg gccttgagtt cagccgtacc gctcgtgaag
     2941 gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgtgtca
     3001 ttttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc attaaccgcc
     3061 tgctgcggat gccgctgggt cgcgcctggg aagcgttgcg tgaagatgaa atcgcctgcc
     3121 gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccatc agcgccgcgt
     3181 ttgccggttt tgccggaacg ctgtttgctg cgcgtcaggg ctttgtcagc ccggaatcct
     3241 tcacctttgc cgaatcggcg tttgtgctgg cgatagtggt gctcggcggt atgggctcgc
     3301 aatttgcggt aattctggcg gcaattttgc tggtggtgtc gcgcgagttg atgcgtgatt
     3361 tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc
     3421 cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag
     3481 gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc gcttcggcgg
     3541 cctgctggct gtgaataacg tcaatcttga attgtacccg caggagatcg tctcgttaat
     3601 cggtcctaac ggtgccggaa aaaccacggt ctttaactgc ctgaccggat tctacaaacc
     3661 caccggcggc actattttac tgcgcgatca gcatctggag ggtttgccgg ggcagcaaat
     3721 cgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgcg aaatgacggt
     3781 aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt tctctggcct
     3841 gttgaaaacg ccttctttcc gtcgcgccca aagtgaagcg ctcgaccgcg ccgccacctg
     3901 gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc tggcctacgg
     3961 tgaccagcgc cgtctggaga ttgcccgctg catggtgacg cagccggaga ttttaatgct
     4021 cgacgaacct gcggcaggtc ttaacccgaa agaaacgaaa gagctggatg agctgattgc
     4081 cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata tgaagctggt
     4141 gatgggaatt tcggaccgaa tttacgtggt caatcagggg acgccgctgg caaacggtac
     4201 gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga
     4261 tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa atccaggcgc
     4321 tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cacgctgatt ggcgcgaacg
     4381 gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc accagcgggc
     4441 gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag
     4501 cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc
     4561 tggcgatggg cggctttttt gccgaacgtg atcagtttca ggagcgcata aagtgggtgt
     4621 atgagctgtt tccacgtctt catgagcgtc gtgttcagcg ggcaggcacc atgtccggcg
     4681 gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt ttgttgttgc
     4741 ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc gacaccatcg
     4801 agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc aaccaggcgc
     4861 tgaagctcgc tgatcgcggc tacgtgctgg aaaacggcca tgttgtgctc tccgatactg
     4921 gtgatgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg taaattatcg
     4981 ataacccggc agctaagttc tgccgggtta ttcgtccgta tacagacttg tttcaactga
     5041 ccgggaggat taaaaccaga ccacttattt gccacggaaa ggttccctcc catgcattat
     5101 gccgttgttg tcatgcttga actgctttgc tgtattcacg cttaccgtac cggacaagag
     5161 cggtactgga tttttatcat tttctgtttt ccggtgattg gctgcgtggc gtatttcgtc
     5221 atagtgatgc tgcctgaaac gggcgctgac cgtcacgggc atactttgct gatgcgcctg
     5281 caggacaaac tcaaccctga gcgccacctg cgcaaattaa ccgaagaact ggcgattgcc
     5341 gagacgaatc aaaaccatta tgcgttggct aatgaactgg cgcgtctggg gcgttatcac
     5401 gaagctgttc cccattatca gcaggcgtta agcgggatat tcgcccatga agcggcaatg
     5461 atgctaagcc tggcgcaggc acagtttgct attcaggagt tcgccgcctg tcagcagacg
     5521 ctggaagatg tgatgcgtta taaccctgat tttcagtcgg cagacgggca tttgttattt
     5581 gccagaacgt tagccgcaca agaaaaatac gctgatgcgg aaagcgagtt tgaagtattg
     5641 atttcttatt accccggccc gcaggcgcgt atttactatg cggaaatgtt ggcgaaaatg
     5701 agtcggttac gtgaagccaa cgagcagtat gttgccgtgg ttgatacggc taaacgctca
     5761 agacctcatt atagaaaaca ccaccgggaa tggattaaga ccgccaatga gcggctgaag
     5821 cagagtgtgg tgcaatgaaa atcttctttg ccgttagacc agtgtcgcgg caatcttttg
     5881 ttaaaattga aaaatgtagc tctcatcggg cagcaaaatt ggacgcagaa tttgctgcca
     5941 tttttgctct tcatggtaag gagttctggc gattagctgg ccgttaatcg cctcttttca
     6001 gtgcattctc gggatatccg tgccccatct cccattttcc ctcacccgta ccgtcaccgc
     6061 cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt
     6121 aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac
     6181 aacaagagag ataaacgatg aaaccgttac attatacagc ttcagcactg gcgctcggac
     6241 tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag
     6301 gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa aacccggatt
     6361 acaaaattgt accgacctat aaaggcaatt acgaacagaa tttaagcgcg gggattgccg
     6421 catttcgtac cggcaatgct ccggctattt tgcaggttta tgaagttggc accgccacca
     6481 tgatggcgtc gaaagccatt aaaccggttt atgacgtgtt taaagaggcg gggattcaat
     6541 tcgatgagtc gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc
     6601 acttactctc ccagccattc aacagctcga ctcccgttct ctattacaac aaagacgcct
     6661 tcaagaaagc ggggttagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact
     6721 atgccgcaaa actaaaagcc tccggcatga agtgcggcta cgccagcggc tggcagggct
     6781 ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg
     6841 gctttgacgg cacggatgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacatcg
     6901 ccatgctcga agagatgaac aagaagggcg atttcagcta cgtcgggcgt aaggatgaat
     6961 ccaccgagaa gttctataac ggtgattgcg ctatgaccac cgcctcttcc ggttctctcg
     7021 ctaatattcg cgagtacgcc aaattcaact atggcgtagg catgatgcct tacgacgccg
     7081 atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg
     7141 gcaaagataa agaaacgtat accggcgtgg cgaagttcct cgacttcctc gcgaagccag
     7201 aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg
     7261 acctgacccg tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc
     7321 agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc
     7381 agatccgcgt gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac
     7441 agcaggcact ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat
     7501 cgacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg
     7561 tgtaggccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca
     7621 tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc
     7681 atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctgcaaagc
     7741 gtcgatccgt ttggtttctc cagccggttt gtcggcctgg ataacttcgt cgcgctgttt
     7801 catgacagct actatatcga ctccttctgg acgacgataa aattcagcac ctttgtcacc
     7861 gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc
     7921 agtcgtttct atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc
     7981 gtattgtgga tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgctgag
     8041 ttcggctacg actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt
     8101 gcctcagtat ggaagcaaat cagctacaac ttcctgttct tctatgccgc gctgcaatcc
     8161 attccccgtt cgttgatcga agccgcagcc atcgacggtg cagggccgat tcgccgcttc
     8221 tttaagattg cgctgccgct tatcgccccg gtgagtttct tcctgctggt agtgaacctg
     8281 gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt
     8341 caggccacca ctacgctgat ttataagatc taccgcgaag gctttaccgg actggatctg
     8401 gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtgatg
     8461 cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga
     8521 cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctattc ccgctgtacg
     8581 tggcgtttgt cgcggcgacg ctggataaac aggccgtcta cgccgcgccg atgacgctca
     8641 tccccggcac gcatctgctg gaaaacatcc acaacatctg ggtaaacggg gtaggcacaa
     8701 atagtgcgcc gttctggcgg atgttgctta acagcttcgt gatggcgttc agcattacgc
     8761 tcggcaaaat taccgtctcg atgctctcgg cattcgccat tgtctggttt cgttttccgc
     8821 tgcgtaacct ctttttctgg atgattttta tcaccctgat gttgccggtt gaagtacgta
     8881 tcttcccgac ggtggaagtc atcgccaacc tgaagatgct cgacagctac gcaggtttaa
     8941 cgctgccgct gatggcctcg gcgaccgcga cgtttctttt ccgccagttc tttatgaccc
     9001 taccggatga actggtggaa gccgcgcgga tcgacggcgc gtcgccaatg cgcttctttt
     9061 gcgacatcgt ttttccgttg tccaaaacta atctggcggc gttgtttgtg atcaccttta
     9121 tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg gatctcggca
     9181 ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccaca gaatggaact
     9241 cagtgatggc ggcgatgttg ttaaccctga tcccaccagt agtgattgtt ttagtgatgc
     9301 agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta
     9361 caggcagtaa ccaaaagctg ggatggcaaa acccaggtga ttaaaccgct gacccttgat
     9421 gtggcggatg gcgaatttat cgtgatggtc gggccgtcag gctgcgggaa atcgacgctg
     9481 ctgcgcatgg ttgccgggct ggagcgggtg acggaaggcg atatctgtat caacgaccag
     9541 cgggtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaactacgcg
     9601 ctttatccgc atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg
     9661 ggcaagcagc aaattgccga gcgcgttaaa gaggcggcgc gcattctgga actggacggt
     9721 ctgcttaagc gccgcccgcg cgagctttcc ggcggtcagc gtcagcgtgt ggcgatgggc
     9781 cgggcgattg tgcgcgatcc ggcggtgttc ctgtttgatg agccactctc taacctcgat
     9841 gccaagctgc gcgtacagat gcgtcttgaa ctgcaacagc tgcaccgtcg cctgaaaacg
     9901 acttcactct acgttactca cgatcaggtt gaggcgatga ccctcgccca gcgagtaatg
     9961 gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc
    10021 gccagcctgt ttgtggcgag ttttattggc agcccggcga tgaatctgct gacaggccgc
    10081 gtgaataacg aaggcacgca cttcgagctg gacggcggca ttgcgctgcc gctaaacggt
    10141 ggctaccgtc agtatgcagg gcgtaaaatg actctcggca ttcgcccgga acatatcgcg
    10201 ctaagctcgc aggcagaagg cggcgtgccg ctggtgatgg acacgctgga gatcctcggc
    10261 gcagataacc tggcgcacgg acgctggggc gaacagaagc tggtggtacg gctggcgcat
    10321 caggagcgcc cgacggcagg cagcacgctg tggctgcatc tgccggaaaa tcagctacat
    10381 ctttttgatg gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg
    10441 cccaccgtgg cggcggtaaa ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg
    10501 caaaatacgg tcataagatg atcgaatttg acgcgaagtt gtcgaaagat ggcgaaatct
    10561 tcctgctcca tgacgacaat ctcgaacgta ccagcaacgg ctggggcgtc gcgggtgaac
    10621 tgaactggca ggatttacta cgtgtggatg caggcagttg gtacggcaaa gcgtttaaag
    10681 gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat gggatgatgg
    10741 cgaatatcga aatcaaaccc accactggca ctgggccatt aacgggcaaa atggtggcgc
    10801 tggcggcacg cgagctgtgg gccggtatga cgccgccgct actgtcatcg tttgagattg
    10861 atgctttaga agccgcacaa caggcagtac cagaactgcc gcgcggtttg ttgctggatg
    10921 agtggcgcga cgactggcga gaactgaccg cgcggctggg ctgcgtctct attcatctca
    10981 atcataagtt gctcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc
    11041 tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt
    11101 gcatctgtac cgatgcgatt gatgtgattg gtccgaactt tacggcccaa tagttttcaa
    11161 cggtatatca ggctgcggcg tgccgggctg attcagcatg tcgccgtttc tcctctccgg
    11221 caacatatgc tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc
    11281 attggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa
    11341 cacccgctga gaattattgt ttatctggtt ttctaaatgc tgttgttgca actgagtttg
    11401 cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgcgttt gcatctgggt
    11461 ttgcatccgc tgctgactgg ggatctgata ccccggctgg ttagggttgt tcagagtatt
    11521 aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat
    11581 cgcgtatcct cctctgaaga tattctttaa gtttactcct ttcctgcaat catcaggatt
    11641 aattcagagt tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga
    11701 tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgttcaca ggaagttgtt
    11761 ttagtacggt agccgcgcca cctgcgccgt ctcctgtttc ttacggcgtg gaggaagatg
    11821 tctttcatcc ggtacgcgcg aaacagggga tggtggcatc tgtagatgct actgccactc
    11881 aggtgggggt ggatatcctc aaggagggcg ggaatgccgt tgatgccgcc gtggctgtgg
    11941 gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt ggttttatgc
    12001 tgatccgctc gaaaaatggc aataccaccg ctatcgattt ccgcgaaatg gcacccgcca
    12061 aagcgacccg cgatatgttc cttgatgacc agggcaaccc ggacagcaaa aaatcactca
    12121 cttcgcatct ggcttccggc acgcccggta cggtcgctgg tttctcgctg gcgttggata
    12181 aatacggcac aatgccgttg aacaaagtgg tgcagccagc gtttaaactg gcacgcgatg
    12241 gttttatcgt taacgacgag ctggctgacg atctcaaaac ctacgggagc gaagtgctgc
    12301 cgaatcacga aaacagcaag gctatcttct ggaaagaggg cgagccgctg aaaaagggcg
    12361 acaagctggt gcaggcgaac ctggcaaaga gcctggagat gattgccgaa aacggcccgg
    12421 acgaattcta caaaggcacg atagcggaac agattgccca ggagatgcag aaaaacggtg
    12481 gcctgatcac taaagaagat ttagcggcgt ataaagcggt cgaacgcaca ccgataagcg
    12541 gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc gggatccata
    12601 tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggt tttggcagcg
    12661 ccgacgcgat gcagatcatg gcagaagcgg agaaatatgc ctacgctgac cgctcggaat
    12721 atcttggcga cccggatttt gtgaaagtcc cgtggcaggc gctgaccaat aaagcctatg
    12781 ccaaatctat tgccgagcaa atcgatatca ataaagcgaa accgtccagc gagattcgcc
    12841 ccggcaagct tgcgccttat gagagtaatc aaactaccca ttattcggtg gtggataaag
    12901 acggtaacgc ggtggcggtg acctatacgc tgaacaccac cttcggtacg ggtattgtcg
    12961 cgggcgagag cggtattttg ctcaataacc aaatggatga tttctctgcc aaaccgggcg
    13021 taccgaacgt ttacgggctg gtgggcggtg atgccaacgc cgtcgggccg aacaaacgcc
    13081 cgctgtcatc gatgtcacca accattgtgg tgaaagacgg taaaacctgg ctggtcaccg
    13141 gtagccctgg cgggagcctg atcatcacta cagtgcttca aatggtggtg aatagcatcg
    13201 attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat cagtggttgc
    13261 cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg ctggaagcaa
    13321 aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt atggttgggc
    13381 cggacggtga gttgtacggc gcatccgacc cgcgctcggt ggatgattta acggcggggt
    13441 actaaggtta gcggccctct tcgtgggaag agggctgttt tcttgaacaa gataaattag
    13501 tgtctggatt gcttttacta ttgctccgta atatataatc ataattatcc tgaatgcttt
    13561 taaggggctt aggttatgct ttcttttcaa gagtatgcag aatagtcttt catttaccgt
    13621 gaaaatagga cgtgcgataa ttacttcctg atattcagga aaggaattaa ctatgctatt
    13681 aaataaactg tagttatagt tcattttaga gtgtaattta ttgatttaaa tatcacgctt
    13741 tgtgttgttt atgtgcataa tggcattcat catgactttg atcgattttg taaagtgttt
    13801 ttaaggggga ataatgaaag caaaatacgt gattctctct gtacttaata aacaagaact
    13861 cttctattta aaatcaagat acaaaga
//
LOCUS       DAZFKY010000124        13870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFKY010000124 DAZFKY010000000
VERSION     DAZFKY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..1076
                     /gene="ybhH"
                     /locus_tag="ACHKCL_003275"
     CDS             24..1076
                     /gene="ybhH"
                     /locus_tag="ACHKCL_003275"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5764176.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1152..2585
                     /gene="ybhI"
                     /locus_tag="ACHKCL_003276"
     CDS             1152..2585
                     /gene="ybhI"
                     /locus_tag="ACHKCL_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5764177.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2768..5029
                     /gene="ybhJ"
                     /locus_tag="ACHKCL_003277"
     CDS             2768..5029
                     /gene="ybhJ"
                     /locus_tag="ACHKCL_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5764178.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5170..6453)
                     /gene="ybhC"
                     /locus_tag="ACHKCL_003278"
     CDS             complement(5170..6453)
                     /gene="ybhC"
                     /locus_tag="ACHKCL_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5764179.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6588..7654)
                     /locus_tag="ACHKCL_003279"
                     /pseudo
     CDS             complement(6588..7654)
                     /locus_tag="ACHKCL_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(7632..7850)
                     /locus_tag="ACHKCL_003280"
     CDS             complement(7632..7850)
                     /locus_tag="ACHKCL_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5764180.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(7890..8057)
                     /locus_tag="ACHKCL_003281"
     CDS             complement(7890..8057)
                     /locus_tag="ACHKCL_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764181.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            8300..8902
                     /locus_tag="ACHKCL_003282"
     CDS             8300..8902
                     /locus_tag="ACHKCL_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764182.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(9113..9334)
                     /locus_tag="ACHKCL_003283"
     CDS             complement(9113..9334)
                     /locus_tag="ACHKCL_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5764183.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(9433..9648)
                     /locus_tag="ACHKCL_003284"
     CDS             complement(9433..9648)
                     /locus_tag="ACHKCL_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5764184.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(9725..9916)
                     /locus_tag="ACHKCL_003285"
     CDS             complement(9725..9916)
                     /locus_tag="ACHKCL_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5764185.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(9889..10071)
                     /locus_tag="ACHKCL_003286"
     CDS             complement(9889..10071)
                     /locus_tag="ACHKCL_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5764186.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(10068..10748)
                     /locus_tag="ACHKCL_003287"
     CDS             complement(10068..10748)
                     /locus_tag="ACHKCL_003287"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5764187.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(10745..11416)
                     /gene="bet"
                     /locus_tag="ACHKCL_003288"
                     /pseudo
     CDS             complement(10745..11416)
                     /gene="bet"
                     /locus_tag="ACHKCL_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            11446..11628
                     /locus_tag="ACHKCL_003289"
     CDS             11446..11628
                     /locus_tag="ACHKCL_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5764188.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            11625..11795
                     /locus_tag="ACHKCL_003290"
     CDS             11625..11795
                     /locus_tag="ACHKCL_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5764189.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            11788..12408
                     /locus_tag="ACHKCL_003291"
     CDS             11788..12408
                     /locus_tag="ACHKCL_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5764190.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            12405..13070
                     /locus_tag="ACHKCL_003292"
     CDS             12405..13070
                     /locus_tag="ACHKCL_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5764191.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            13067..13690
                     /locus_tag="ACHKCL_003293"
     CDS             13067..13690
                     /locus_tag="ACHKCL_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5764192.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT         3458 a         3274 c         3446 g         3692 t
ORIGIN      
        1 gtttttaaat tgagggcatt attatgaaaa aaataccctg cgtgatgatg cgaggtggaa
       61 cctcgagggg cgcgttcctg ttagcggaac atttacccga agatcaaacg cagcgcgata
      121 aaatattgat ggcaattatg ggttccggta acgatctgga aattgacggt attggcggcg
      181 gtaatccact gaccagtaaa gtcgccatta ttagccgttc cagcgatccg cgtgctgatg
      241 ttgattatct gtttgctcaa gttatcgtcc atgagaaacg tgtcgatacc acgcctaact
      301 gcggcaatat gctgtctggc gttggggcat tcgccattga aaatggtttg attgcagcga
      361 cttcgccagt tacccgcgta cgtatccgca acgtcaatac gggtacgttc atcgaagctg
      421 atgtgcaaac gccaaatggt gttgtcgagt acgagggtag cgccagaatt gacggcgtac
      481 cgggtactgc cgcaccggtt gcgctcactt tcctgaatgc cgctggaacg aaaaccggaa
      541 aagttttccc gactgataat cagattgatt attttgacga tgtcccggtg acctgtatcg
      601 atatggcgat gccagtcgtc attattccgg ctgaatatct gggaaaaaca ggctatgaat
      661 taccggcgga actggatgcc gacaaagcat tattagcccg cattgaatct atccgtctac
      721 aagcgggtaa agcaatgggc ttaggcgatg tcagtaatat ggttatccct aaacctgtgc
      781 ttatttctcc agcgcagaaa ggcggggcaa ttaatgtgcg ttattttatg ccgcattctt
      841 gccatcgcgc gctggcgata accggtgcta ttgctatttc cagtagttgt gcattggaag
      901 gtaccgtcac ccgacaaatc gtcccttctg taggatacgg caatatcaat attgaacacc
      961 ccagtggtgg gctcgacgtt catttaagta atgaaggtca ggatgccacg acgttacgcg
     1021 catctgttat tcggacaacc agaaaaatat tttccggtga agtttatctt ccctgaaaag
     1081 attcgttgtc aggataagga caatcaataa aggacttctg tatgagtcat acagaaagaa
     1141 caggatttta aatgaataag aaatcgttat ggaagctaat tctgatatta gcgatcccat
     1201 gtattattgg ctttatgcca gctccggcag gattaagcga actggcgtgg gtgctttttg
     1261 gtatttacct ggcggccatt gtggggctgg ttatcaagcc tttcccggaa cctgtcgtac
     1321 tgttaattgc cgttgctgcc tccatggtgg tggtcggtaa cttatccgac ggtgcgttta
     1381 agaccaccgc cgtattaagc ggttactctt caggtactac ctggctggtg ttctcggcgt
     1441 ttaccttaag cgccgcattt gtgaccaccg gtttaggtaa acgtattgcc tatctgctga
     1501 ttggtaaaat cggtaacacc acgctgggtc tgggttacgt tacggtattc ctcgatctgg
     1561 tattggctcc ggcaacaccg tctaacaccg cgcgtgcggg cggcattgtg ttaccgatca
     1621 tcaacagcgt ggcggtggct ttggggtccg aaccggaaaa aagtccgcgt cgtgtcggac
     1681 attacctgat gatgtccatt tacatggtca ccaaaaccac cagctatatg ttctttaccg
     1741 caatggcggg gaacattctg gcgctgaaaa tgatcaacga cattctgcac ctgcaaatta
     1801 gctggggtgg atgggcgctg gccgccggat tgccgggcat cattatgctg ctggtcaccc
     1861 cgctggtgat ttacaccatg tatccaccag aaattaagaa ggtggataac aaaaccatcg
     1921 ctaaagcggg ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg ctgctcggtg
     1981 tctttgtgct ggcgctgctg ggctggattt tcagtaagtc tctgggggtt gatgaatctt
     2041 cagtggctat tgttgttatg gcgaccatgc tgctgctggg tatcgttacc tgggaagacg
     2101 tggttaaaaa taaaggcggc tggaatacct taatctggta cggcggtatt atcggcttaa
     2161 gctccttatt atcgaaagtt aaatttttcg aatggttagc tgaagtcttt aaaaataacc
     2221 tggcatttga tggtcacggt aacgttgctt tcttcgttat tattttcctc agcatcatcg
     2281 tgcgttattt cttcgcttcc ggtagtgcct atatcgttgc tatgttaccg gtatttgcca
     2341 tgctggcgaa cgtctccggc gcaccgttaa tgttaaccgc gctggcactg ttgttctcca
     2401 actcctatgg cggcatggtt actcactatg gcggcgcggc aggtccggtc atctttggcg
     2461 tgggttataa cgatattaaa tcctggtggt tggtcggtgc ggtactgacg atattaacct
     2521 tcctggtgca tatcaccctc ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc
     2581 tgtaaatata ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag
     2641 tcacttactt atgtaagctc ctggggattc actcgcttgt cgccttcctg caactcgaat
     2701 tatttagagt atatccattt attttcttgc tgcgcacttc acggtgcgca gatatctgga
     2761 gcatttgatg atcaagttat ctgaaaaagg cgtgtttctc gccagtaata acgaaataat
     2821 tgccgaagaa catttcaccg gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc
     2881 ctggtctatt ctctcttctc ataatacgtc cggaaatatg gataaactta aaattaagtt
     2941 tgattcatta gcctctcacg atattacctt tgttggtatt gtacagaccg ctaaagcgtc
     3001 cggtatggaa cgtttcccgc tgccgtatgt gctgaccaac tgccataact cactctgcgc
     3061 cgtcggcggc accattaacg gtgatgacca tgtttttggt ttatcggcag ctcagcgtta
     3121 tggcggtatt tttgtgcctc cgcatattgc ggtcatccat caatatatgc gtgagatgat
     3181 ggcaggtggc ggcaaaatga tcctcgggtc agacagtcac acccgttacg gtgcattagg
     3241 gacaatggca gtcggtgagg gcggcggcga gttggtaaaa caattgctta atgacacctg
     3301 ggatatcgac tatccgggag ttgttgcggt gcatctgacc ggaaaaccag cgccgtatgt
     3361 ggggccgcag gatgtggcgt tggctatcat cggtgccgtg ttcaaaaacg gctacgtcaa
     3421 aaacaaagtg atggaattcg taggtcccgg tgttgctgcg ctctctaccg atttccgtaa
     3481 cagcgttgac gttatgacca ctgaaacgac ctgtttaagt tctgtctggc aaaccgatga
     3541 agaagtccat aactggctgg cgctgcacgg tcgcggccag gattactgcc agcttaaccc
     3601 tcaaccgatg gcgtactacg atggctgcat cagcgttgat ttaagcgcca tcaaaccaat
     3661 gattgcgctg ccgttccacc cgagcaacgt gtatgaaatc gacacactga accagaactt
     3721 gaccgacatt ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa
     3781 actctcgctg ctggacaaag tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc
     3841 gggctgttct ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca
     3901 atcctgtggc aatgacacct tctcgctggc ggtttacccg tcatcacagc cggtgtttat
     3961 ggatctcgcc aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa tcatcagaac
     4021 cgcgttctgc ggcccatgct ttggcgcggg cgatacgcca atcaacaacg gtttgagtat
     4081 tcgccacacc acgcgtaact tcccgaaccg cgaaggctct aagccagcta atgggcagat
     4141 gtcagcggtg gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa acggtggcta
     4201 tttaacctct gccagcgaac tcgattgctg ggacaacgtg ccggagtacg ccttcgatgt
     4261 aacgccgtat aaaaatcgcg tctatcaagg ctttgtgaaa ggggcggccc agcaaccgct
     4321 gatttacgga ccgaacatta aagactggcc ggaattgggt gcgctgactg acaatatcgt
     4381 cctcaaagtg tgctcgaaga tcctcgacga agtgaccacc accgacgaat tgattccttc
     4441 cggtgaaacc tcttcttatc gttcaaatcc gattggtctg gcggagttta ccctgtcacg
     4501 ccgcgatcca ggttatgttg gcagcagtaa agcgactgct gagctggaaa atcagcgtct
     4561 ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga ttgctggtca
     4621 ggagcatatt gatccgctgc aaactgaaat tggcagcatg gtctatgcgg tgaaaccagg
     4681 cgatggttct gcgcgtgaac aggcggcgag ttgtcagcgt gtgattggcg gtctggcgaa
     4741 tattgccgag gagtacgcga ctaaacgcta tcgttctaac gtcatcaact gggggatgtt
     4801 accgctgcag atggtggaag cgccaacctt tgaagtgggg gattacattt acatccctgg
     4861 cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga tccatgaaga
     4921 tgcgccggta acggaaatta cgctctatat ggaaagtctg actgctgaag agcgcgagat
     4981 tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa aagcgccatg
     5041 tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat cttatccgac
     5101 ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc ggcaaatagt
     5161 taatagctct tacttcttcg cctctgcaac cactttgcta cccacgccgc ggttattgta
     5221 ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt cgctatcgtc
     5281 aacgctgccg gtgttacccg caaacggacg attagagatc accgcatcgc cccacggttt
     5341 agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac cgttggtatt
     5401 ggcatcaaca tccagcgagc ggcccagttg tgccacgcca tcaccggaag cattgaaacg
     5461 gctgtttacg gcgaggaaac cgtagtaaat gttggacagc gtagccggtg caaacacata
     5521 cgcttcttgc tgagtacgtg agttcaccac gcggaattcg gtgttatcga acaccactgc
     5581 gccgcgacca gaaacgatat ccacatcccc ttcaatgtag ctgttggtca ccagcgtacg
     5641 cggctgacga ttcgtttcca gacggttctg cacaccgctg ttggtgacaa agaaggtgtt
     5701 ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg tcaccgtcag tacgcagtgc
     5761 caccgccgga tggttacccg catctacgct atcacccagc gtgttttcga tggtcaggtt
     5821 ttgcagttgc aggccattgt tttgtgacca gaagaccgca gaacacagaa caccgatact
     5881 gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac ctggcatata
     5941 tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc catcaaggga
     6001 aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtaa ttccacctgg
     6061 agcagcaggg acatacaccg ttccctgata ctcaccaggc atcacggcaa tatactggcg
     6121 cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg tatgcgttac
     6181 accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg ttatcgggga
     6241 cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgag catcgaaatt
     6301 ctgcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag tttgatcaga
     6361 aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa aagccaatgc
     6421 cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat gaaatagaaa
     6481 aatgaatccg ttgaagtctg cttttttata ctaagttggc attataaaaa agcattgctt
     6541 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgatttca
     6601 attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt gtccgactta
     6661 tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag tcttgcagac
     6721 aaactgcgca actcgtgaaa ggtaggcgga tccccttcga aggaaagacc tgatgctttt
     6781 cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg acgagtagat
     6841 gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc cttcattgat
     6901 atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc tgttttgctt
     6961 tgttcgacat aaagatatcc atctacgata tcagaccact tcatttcgca taaatcacca
     7021 actcgctgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca tggtgatgat
     7081 tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct ccttacctct
     7141 gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc agctattgcc
     7201 tctcggaatg catcgctcag tgttgatctg attaacttgg ctgacgccgc cttgccctcg
     7261 tctatgtatc cattgagcat tgccgcaatt tcttttgtgg tgatgtcttc aagtggagca
     7321 tcaggcagcc ccctccttat tgctttaatt ttgctcatgt aatttatgag tgtcttctgc
     7381 ttgattcctc tgctggcgag gattttttcg tagcgatcaa gccatgaatg taacgtaaca
     7441 gaattatcac tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga aaataactca
     7501 atattggcct gtattgcttc agtgattgct atcctcctgt ctcggcctaa accaaactct
     7561 ttacccgtcc ttgggtccct gtagcagtaa tattgtttct tatataaagg ttagggggta
     7621 aatcccggcg ctcatgactt cgccttcttc ccatttctga tcctcttcaa aaggctacct
     7681 gttactggtc gatttaagtc aacctttacc gctgattcgt ggaacagata ctctcttcca
     7741 tccttaaccg gaggagggaa tatcctgcat tcgcgcaccc atcgacgaac tgtttcaagg
     7801 cttcttgggc gtcgctggcg tgcgttccac tcctgaagtg tcaagtacat cgcaaagtct
     7861 ccgcaattac acgcaagaaa aagccgccat caggcggctt ggtgttcttt cagttcttca
     7921 attcgaatat tggttatgtc tgcatgtgct atctgcgccc atatcatcca gtggtcatag
     7981 cagtcattga tgttctctgc ttcgataact ctgttgaatg gttctccatt ccattcacct
     8041 gtgactcgga agtgcattta tcatctccat aaaacaaaac ccgccgtagc gagttcagat
     8101 aaaagaaatc ctcgtcagtg cgaggatgct gttcattgct gctatacact tttttgctct
     8161 caacgtaagc ggtagctcat tctgttgggt tggtgcagtt gcttttagga aatgctattt
     8221 accccttaaa cgtcggctga aagagctaaa atccatgcaa aaaatttacg caattttgtg
     8281 tattattgtg cagtaagtaa tgagctattt tctgcgcaaa aaatggatgg taaatttgtc
     8341 cggggcagga aaaattttat gggcgctaaa catgaaaaaa gattcgtatc cttatttgat
     8401 ttgcatgaca gttttaggcc tgatctttat tttccttttc ttctggtggc gggcagatat
     8461 ttacagggtc acgtttctta atcagagtat atcccactat tacattctgt ttagcatggg
     8521 aatagctttt ctgttatctc tgttttgggt taagaagggg atagtaaaac aaagcggctg
     8581 gaagagtctg tcagcatacc ttaaggttta tgcagggatg tgcatatttg ctggattttt
     8641 tctgattata ccccttacaa cactaactta ttttttgcct ggagagacat cgtcttatgt
     8701 tgcaccgtat cggtatactt ccggtagttc aaaaagttgt tctggagctg aggtggatga
     8761 ccccgatcta catgagaata ttcgcatttg ctatccgtat ggcaattatg agtacgataa
     8821 tattatctat gttgaaaaga aaattaatac attaggtgcg gtagtaacat atgcacagac
     8881 cgcgcgtgat gatactgaat gagatagtat atagcgggca agttttagtt aatttatcga
     8941 ggtgatataa tttacctcga ctcgtttatt ctggtattaa tatttcgctt tacgatcgat
     9001 ttttatctga tgatatcatg cggttttcat atactgactt actgtctttt ctccgttagc
     9061 gattttctcc tgctcagcga tgattttatc tttggcttct agttaatttc gctcacttcg
     9121 aacctctctg tttactgata agttccagat cctcctggca acttgcacaa gtccgacaac
     9181 cctgaacggc caggcgtctt cgctcatcta tgggatcgcc acactcacaa caatgagtgg
     9241 cagatatagc ctggtggttc aggcggcgca tttttattgc tgtgttgcgc tgtaattctt
     9301 caatttctga tgctgaatca atgatgtctg ccatcttcca ttaatccctg aattgttggt
     9361 taatacgctt gagggtgaat gcgaataata aaaaaggagc ctgtagctcc ctgatgattt
     9421 tgcttttcat gttcaccgtt ccttaaagac gccgtttaac atgccgattg ccagacttaa
     9481 gtgagtcggt gtgaatccca tcagcgttac cgtttcgcgg tgcttcttca gtacgctacg
     9541 gcaaatgtca tcgacgtttt tatccggaaa cggctgtctg gcttttttga tttcagaatt
     9601 agcctgacgg gcaatactgc gaagggcgtt ttcttgctga ggtgtcattg aacaagtccc
     9661 atgtcggcaa gcataagcac acagaatatg aagcccgctg ccagaaaaat gcattcagtg
     9721 gttgtcatac caggtctctc tcatctgctt ctgctttcgc caccatcatt tccagctttt
     9781 gcgaaaggga tgtggctaac gtatgaaatt cttcgtctgt ttctactggt attggcacaa
     9841 acctgactcc aatttgagca aggctatgtg ccatctcaat actcgttctt aactcaacag
     9901 gagatgcttt gtgcatatcg cctcccgttt attatttatc tcctcagcca gccgctgggc
     9961 tttcagcgga tttcggataa cagaaaggcc gggaaatacc cagcctcgct tcgtaacgga
    10021 gtagacgaaa gtgatcgtgc ctacgcggat attatcgtga ggatgcttca tcgccattgc
    10081 tccccaaata caaaaccaat ttcagccagt gcctcgtcca ttttttcgat gaactccggc
    10141 accatctcgt caaaacccgc catgtacttt tcatcccgct caaccacgac ataatgcagg
    10201 ccttcacgct tcatacgcgg gtcatagttg gcaaagtacc aggcatcttt tcgcgtcacc
    10261 cacatgctgt actgcacctg ggccatgtaa gccgacttta tggcctcgaa accaccgagc
    10321 ctgaacttca tgaaatcccg ggaggtaaac gggcatttca gctcaaggcc gttgccgtca
    10381 ctgcataaac catcgggaga gcaggcggtg cgcatacttt cgtcgcgata gatgatcggg
    10441 gattcagtaa cattcacgcc ggaagtgaat tcaaacaggg ttctggcgtc gttctcgtac
    10501 tgttttcccc aggccagcgc cttagcatta acttccggag ccacaccggt gcaaacctca
    10561 gccagcaggg tgtggaagta ggacattttc atgtcaggcc acttctttcc tgagcggggc
    10621 tttgctatca cgttgtgaac ttctgaagcg gtgatgacgc cgagccgtaa tttgtgccat
    10681 gcatcatccc cctgttcgac agctctcacg tcgatcccgg tacgctgcag gataatgtcc
    10741 ggtgtcatgc tgccaccttc tgctcagtgg ctttctgttt caggaatcca agagctttca
    10801 ctgcttcggc ctgtgtcagt tctgacgatg cgcgaatgtc gcggcgaaat atctgggaac
    10861 agagcggcaa taagtcgtca tcccatgttt tatccagggc gattagcaga gtgttaatct
    10921 cctgcatggt ttcatcgtta accggagtga tgtcgcgttc cggctggcgt tctgcagtgt
    10981 atgcagtatt ttcgacaatg cgctcggctt catccttgtc atagatacca gcaaatccga
    11041 aggccagacg ggcacactga atcatagctt tatgccgtaa catccgtttg ggatgcgact
    11101 gccacggccc cgtgatttct ctgccttcgc gggttttgaa tggttcgcgg cggcattcat
    11161 ccatccattc ggtaacgcag atcggatgat tacggtcctt gcggtaaatc cggcatgtac
    11221 aggattcatt gtcctgctca aagtccatgc catcaaactg ctggttttca ttgatgatgc
    11281 gggaccagcc atcaacgccc accaccggaa cgatgccgtt ctgcttatca gggaaggcgt
    11341 aaatttcttt cgtccacgga ttaaggccgt actggttggc gacgatcaac aatgcgatga
    11401 actgcgcatc gctggcatga aagcgcgatg gggagacagg gcggcatgag acgacagcga
    11461 cgaagtttca ccgacatcat ctgcgaaaac tgcaaatacc ttccaacgaa acgctccaga
    11521 aataaacgca agccaatccc aaaagaatct gacgtaaaaa ccttcaacta cacggctcac
    11581 ctgtgggata tccggtggct aagacattgt gcgaggaaaa caaggtgatt gaccaaaatc
    11641 gaagttacga acaagaaagc gtcgagcggg ctttaacgtg cgctaactgc ggtcagaagc
    11701 tgcatgtgct ggaagttcac gtgtgtgagc actgctgcgc agagctgatg agcgattcga
    11761 atagctcgat gcacgaggaa aaagatgatg gctaaaccag cgcgaagacg atgtaaaaac
    11821 gatgaatgtc gggaatggtt tcaccctgca ttcgctaatc agtggtggtg ctctccagag
    11881 tgtggaacca agatagcact cgaacgacga agcaaagaac gcgaaaaagc ggaaaaggca
    11941 gaaaaggcag cagagaagaa acgacgacga gaggagcaga aacagaaaga taaacttaag
    12001 attcaaaaac tcgccttaaa gccccgcagt tactggatta aacaagccca acaagccgta
    12061 cacgccttca tcagagaaag agaccgcgac ttaccatgta tctcgtgcgg aacgctcacg
    12121 tctgctcagt gggatgccgg acattaccgg acaactgctg cggcacctca actccgattt
    12181 gatgaacgca atattcacaa gcaatgcgtg gtgtgcaacc agcacaaaag cggaaatctc
    12241 gttccgtatc gcgtcgaact gattagccgc atcgggcagg aagcagtaga ggaaatcgaa
    12301 tcaaaccata accgctatcg ctggactgtc gaagagtgca gggccatcaa ggcggagtat
    12361 caacagaaac ttaaaaaact gcgaaacagc agaagtgagg ttgcatgaat atctacgaaa
    12421 gaattgatgg cagcaaatac cgaaatattt gggtagttgg cgatctgcac ggatgctaca
    12481 cgaacctgat gaaaaaactg gagacgatag gattcgacac caaaaaagac ctgcttatct
    12541 cggtgggcga tttggttgat cgcggtacag agaacgtaga atgcctggaa ttaatcacat
    12601 tcccctggtt cagagctgta cgtggaaacc atgagcaaat gatgattgat ggcttatcag
    12661 agcgtggaaa cgtcaatcac tggctgctta atggcggtgg ctggttcttt aatctcgatt
    12721 acgacaaaga aattctggct aaagctcttg cccataaagc agatgaactt ccgttaatca
    12781 tcgaactggt gagtaaagga aaaaaatatg tcatctgcca cgccgattat ccttgtgata
    12841 aatacgagtt tggaaagcca gttgatcatc agcaggtaat ctggaaccgc gaacgaatca
    12901 gcaactcaca agacgggatc gtgaaagaaa tcaaaggcgc ggacacgttc atctttggtc
    12961 atacgccagc agtgaaacca ctcaaatttg ccaaccagat gtatatcgat actggcgcag
    13021 tgttctgcgg aaacctcaca ttgattcagg tacagggaga aggcgcatga gactcgaaag
    13081 cgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcaccac gggccacggc
    13141 ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc aatcacaagc
    13201 cggattcgga atggctgtat tctgtggtaa gcacgaactc agccagaacg acaaacaaaa
    13261 ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc gtggcgtggc
    13321 aaagcttgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat tcgcttatgc
    13381 ggattattgc cgtagtgccg ctacgccggg cgcaagatgc agagattgcc acggtacagg
    13441 ccgtgcggtt gatatagcca aaacagagca gtgggggata gttgctgaga aagagtgcgg
    13501 aagatgtaaa ggcgtcggtt attcaagaat gccagcaagc gccgcatatc gcgctgtgac
    13561 gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc cgctgtatga
    13621 cgctctggtt gtgcaatgcc acaaggaaga gtcaatcgca gacaacattt tgaatgcgat
    13681 cacacgttag cgccatgatt gccacggatg gcaacatatt aacggcataa tattgacttt
    13741 ttgaataact ttggggaaac ttgacaccaa taatgggcgt tttttacatg tcattgatga
    13801 gtctcaataa cctgccgccg agtagttttt atgctctgaa ttgtatttgt gtagtaaaca
    13861 tgctgactgc
//
LOCUS       DAZFKY010000125        13833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFKY010000125 DAZFKY010000000
VERSION     DAZFKY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            112..456
                     /locus_tag="ACHKCL_003294"
     CDS             112..456
                     /locus_tag="ACHKCL_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO5764193.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSIWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(1007..9388)
                     /locus_tag="ACHKCL_003295"
     CDS             complement(1007..9388)
                     /locus_tag="ACHKCL_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO5764194.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDIVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYNDSAVWVAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTVSAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMGVSPWEGVADATIEGAAFGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     TKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGDMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDKVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASFQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKL
                     SRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKSMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEIAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVD"
     gene            complement(9458..10723)
                     /locus_tag="ACHKCL_003296"
     CDS             complement(9458..10723)
                     /locus_tag="ACHKCL_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764195.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGDKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(10734..10985)
                     /locus_tag="ACHKCL_003297"
     CDS             complement(10734..10985)
                     /locus_tag="ACHKCL_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO5764196.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(10995..11441)
                     /locus_tag="ACHKCL_003298"
     CDS             complement(10995..11441)
                     /locus_tag="ACHKCL_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764197.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(11444..12100)
                     /locus_tag="ACHKCL_003299"
     CDS             complement(11444..12100)
                     /locus_tag="ACHKCL_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764198.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(12194..12595)
                     /locus_tag="ACHKCL_003300"
     CDS             complement(12194..12595)
                     /locus_tag="ACHKCL_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764199.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(12652..12792)
                     /locus_tag="ACHKCL_003301"
     CDS             complement(12652..12792)
                     /locus_tag="ACHKCL_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5764200.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(13025..13759)
                     /locus_tag="ACHKCL_003302"
     CDS             complement(13025..13759)
                     /locus_tag="ACHKCL_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5764201.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
BASE COUNT         3017 a         4065 c         3157 g         3594 t
ORIGIN      
        1 gcgggggagt aatctcccgc cacctctgat gtgtcaggca tcctcaacgc acccgcgctt
       61 taccatactg aaaatgctgt ttgaatgttc atctctgaaa gaggactatg aatgaaaaag
      121 gtattgattg cagcacttat ttctggtgtg tcttttggcg cttttgcaca gcagggcggt
      181 ttccaagggc ctgaagcaga gcgttcaaca gtagcgcagg caaaagaact gaaggatgat
      241 gcatgggtta tccttgaagg gagcatcgtt aaaaaagtgg gtgatgaacg ttatgagttt
      301 cgtgacaata gcgggacaat tgtcacggat atcgatgaca gcatatgggc cggtcagaat
      361 gtttctccga aagacaaagt aagaattgag ggtgaaattg ataaagacct gagcagtgtt
      421 gaagtggatg taaaggcact gaaattatta aagtaaccgt cccagcttgc taagcccgtc
      481 ttactgacgg gttttctgtt tgttatcaca gtatttttca acaaaacacc gaatcttatc
      541 cggtgcgctg taaaaccccg tccttcaggg cgtggaggat gtcaaatggc gtatatttgg
      601 atataagaaa gccatccaat actggctgaa aaaactcctc atcgatatca tcgtgggcct
      661 gaatagttcc atccgcaatt ctgattgccg gtattgttcc ccggcgataa ggggtgaaca
      721 tcatcccggt tacatcagat cgcctaataa catatcaaga ttattttgct cataaatgct
      781 attcgccatt accgttgctg cttcagctta atgaaacgca tagatccgcc atgctttttc
      841 agcaaatacg ctgccatttg cgcaactttt tcttcattaa acataaatcg gctctctgta
      901 tcacatgaaa tgctcttctg actatatgtt aatagctccg gttattacag gtcaatgagt
      961 gtttatttct taatcagccc cgcacttccg tacggggcca tttctgttaa tccactccca
     1021 cttttttgtt gtactcgcgg ttaaaccgat ccacggcaat tttcatatcc cgctctaccg
     1081 atttcaccat cgccgactgt tgcgccagac tgagagtact gtcggcgtaa atggcatcac
     1141 gctgtttacg caaatccttc agtctcttcc gggtatcctg cataagcccg ttcatcgaca
     1201 attttccgtt gttctcgtca atgaacgccg ttctttctgc accggtcaga ctcttcagct
     1261 ctgcgtgata ctgcgcaatc tctgtcatcc ggtcgtacat cttctgctgg tctgcatacg
     1321 gcatcacctc ccccgaaatt ttccccagga agggcacctg ctgttccggt atatcaatac
     1381 cattcagcga tttcaccgcc gcatccgtgg ttttggaaat gaagcgccct gtccctccgg
     1441 agatatagtc aatccagaat ttcagcgatt ccggtgtgat atccaccgcg ccgggacggt
     1501 actggctgcc acctgagaac gcattcagcc aggatgcaaa cgccttgtac gcttctggcg
     1561 ttgaacgtct tcccagctgg ctgtcaggtt ttggtgtacc aaacggcatg ttctcctggt
     1621 aaatctgcgc ccccatgaag ttttcattca tggcaaggtt cgcaaacgga cgcagaatgg
     1681 tcggcgctgc atttttcagc aatgccccgg acagtgtttc cgacgtctca ctgccaatcg
     1741 ggctgaatgc accaagcaca ccaccaacaa cattaccggc agcacgggac gccgtcaggt
     1801 cacccgccgc cacaccttca gcagtatgcc cgagcaggaa gaaaacgttg tacccgtaag
     1861 gcagaggaat actccagtac tctccggcct tgcccccgaa caccgatttc ataatgacga
     1921 ggttacgctc tttcacatga gacggcacct tgtcatacca gttaaccccg tcatcatcct
     1981 cacccgccac gctgcggtta agcgagccaa gcagataacc tgctcccaca gcggcaagcg
     2041 cgattttctg cggaacattc agattcttcc agcgaaggcg ctccagtaaa ggtccctcac
     2101 cattaagatg tccgagcgtt ctcaccagat ttgccgttcc ctgaatgctg gcgttggcga
     2161 acatgtacag cgaattcatc agcgccccct gctcaccacg acggttaaag ttcaccgtca
     2221 tgtttttggc aagagacgcc gcctgctggc gtgacaaacc ggcatcacgg gcgtgcttat
     2281 aggcagaaag acgcagagcg ttttcaaccg caccgttggc atcctcgaca aggttaagga
     2341 acgaattcca tgcaccgata ctctggcctt tccatccccc cttcgccagt gagacaaggc
     2401 gatccatttc cttctgctgg ccttcaaggt cacccatgtt aaaccagccg gttttccctc
     2461 cgtcctcaac aaactctttc cacaccttct gccactgtgc accttttccc gtgagggttt
     2521 taccacgcag actggcgtat acggctgaca tggcagaacg gctgtctttc accacagcca
     2581 gggcggataa gttatccagc cctttcagtt taccgtcgct ccttccctgc tccgccttca
     2641 ggttcatcac cgccgtctgc acgtcacgga tgaagttact gaccaggaat tccgggttat
     2701 acgacgtgtt caccgttgcc aggaagcggt taactttccc cagcgtacga acaaaggcat
     2761 tgctggtttc cggtcccata ctcttcatcg cacgcatcag gcgcggatca tggagtttga
     2821 tgtagtacgt tttgccgttc tttttggtgg tgaagtaccg gtctgccatc attgccatcg
     2881 gtacagggcg ttcgacaact tcgcgaatgg tttcaccagt ttcctggtcc ttgcgctctg
     2941 caatcgtccg catggtatcc ggtctgtcat cggtgaatac ctgccagtaa tccttgtcgg
     3001 gattatcctg caccagtttc aggaaggcgt tacccacttc gtttttgcga tggcggatca
     3061 gcgattcgct caggtcctgt atcgcctgag tggacggaga ctgtgcccgg gatgcacgcc
     3121 ccatggcctg cctgctttca cgtccgccga tggtgaagcc cttacctgta cggggcagtg
     3181 acaccacacc gtcaacatcc tgacctttca ggggaacgta gtaacggtag gcgttctgcc
     3241 aggcatccac cacaccgttc tcttcaagtc cggcctcacg gataagctca cggcgacggg
     3301 ccagcatatc gtcaataatc cctgccagac ggtcatactg tgcctgtttg ccactgttac
     3361 gtacacgctg catgatttcc gccgcttccg cgttggtcat ccccgaaccg ccgtccggca
     3421 ttttcgggtt gattttcgcg atatgcgcgt tacgttccgg cgcgtgacgg gcgtagaggt
     3481 actcatccag atcggcctgc gcaattttgt agtccgccag taatttagcc agtggctgaa
     3541 cgtagcgctc cttcatcacg ttcaggtcgt tttccgcctt cccgtggaag agttcttccg
     3601 ccatataagc gttgttactg tcgtccactt tgccgccagt tttacggata ttctcctgaa
     3661 cagctttcag cacctggaat ttatcctgca tctggcgcac aaaacgcgat gcaattgtct
     3721 cttccggtgt cagactgctg gtacgggagt aatacggccc cttgcgaata tcttcaggat
     3781 agagtatttt atccgcagag ccgcgataag aatgttcccc tttgggcaat tggagccctc
     3841 tgtgcagaag gttatcgcgg cttaattttt ctctccggta caacgttaac ccggtcattt
     3901 ccatactctt cagttttttt ccattttctg taccgtaaac agaagctacc ttgttgattt
     3961 ccagacgcga ccggacagcc ttcatgtgta ccgctgacac caccggatca ccatttttat
     4021 ccacggcatc aagcagcatc acaaccgcat ttctttctgt cgctgagcgg taaattgcat
     4081 ccggatcgtg catcagttcc ggtagtctct cgataacatc catcggcacc acatgtttca
     4141 caccattggt ggccttccgc acagtatcgc gggaaataac cagcggcaaa tccggtgcac
     4201 caaggtgacg caataccggc ggcgtacgcc cgatgtttac cgttaaatct gtggtgcgca
     4261 gagatttcat cattctggca aggtcatcac gataacgctc gccctcacct tccggcactt
     4321 tgaacggatc acgtttacca ctacgggagt actgaaacga tgcgcccgcg ccatcctcac
     4381 gcggcgtgta accttcccgc acacgctggc ctaacgtacg gatggtctcg cgaacaagtc
     4441 tgatatcgtt cagttccgtc ggcttcagta accccgtacg acgcagtacc cctttgacca
     4501 gggcaacaac acgctcccat gccgccacga atttattcgg ctgtttctcc gccatatgtg
     4561 ccagaaattc acccgcctgt acttccggtg attccttacc ataggaagca tcaaccttac
     4621 gccaggcttc acggatggtg acgttatcac tgtcgcgggt tttcagcacg gttttgataa
     4681 tcgtctgata ttccgctggt gtgacaacat gctccatggc atggtgaatg atctcgtgac
     4741 gcagcttctc gcgaacggtc cgcccgtcag ggatgttatc cgccaccagg acaatttctc
     4801 gtttatccgg acgatagaat gcgtgcaccc tgccgtaacc atcgaacaat tcacccgcca
     4861 gcgcttcagc ctctttctgt gacttcacca cacggacctt caggtcactg tccttaatgc
     4921 cgctcatcac gccacgggca accgcttcaa cctgcgggac cgggctgcct ttggcttccg
     4981 cactacggtt aacatccgaa atgagattac cttcaggtgt gcgggtaacg cccttacggg
     5041 aataaaacgc aacgcccttg tccgtctcac gggttttcag ggtgcggaac aggtgatcga
     5101 atgcctcacg aataccgcca tccagttccg cattcgtcgg ataagcgtag gtgttatctg
     5161 tgttgtgctc aggtgcctta cggatattga ccagataatc attctccacg ccagccatac
     5221 gcgccttatc ctgaacataa cgctcaaagg cacgtgccgc catttcaaca tccgttgacc
     5281 agtacggttt tgagcgcacc ttatcgagaa gcgctgaacg acgcggcatg tcactgtttt
     5341 taatggcctg aatcacacct ttaaaagcgt cgtaaacctc ctgacgtacc ggatattcag
     5401 catcaacata cctgccgtct ttaaatatgc gcctgacacg ctgtgcttcc gtcataaagt
     5461 cgccacctga cgtaattttc ccgtcattgg aaacgtcata acgaccaaaa taattatcca
     5521 gagaatggaa ccattcgtgc gccagcgcac ccggtccgtt accttttgtc aggttgattg
     5581 ccacctcacc tgactcatag tgtgccgccg ccttaccctt accacgggca ccaaatgcca
     5641 ggccaagacg accgttcagg gaaagcgctt ttgtcggtac attcagtact tccgcaaggt
     5701 catgcagcga gtcataagcc cggttcaaat cagcctgacg acgcggacct tccacgtagt
     5761 taccaaactg cacaccacgg aaaccaaacg catcactgaa ctgctccggt gaaacattcc
     5821 ccttgcggcg ttctggtccg gtacggtcgc ggttggtggc gttgcgctgc tcctcacgcg
     5881 aaatctcccg catctccttc acatgacgaa caagctcatc acgatgtgaa tcaatgtact
     5941 tacgcgcatc actggctgac ttaaagccac ctctcacccg cattttgttt ttaccgtaag
     6001 cgataaaaat atcgccactg cgggtattcc ggtaaacgtc aaagcggatt ttgtcatccg
     6061 gtgacggtgc tgttttttca tcacctttcg cctgtgattt ttcctcctgc tctgcaaacc
     6121 agactttcgc ctttgccagt aattcatccc tgctttccga gaaaaagagg ttactcccct
     6181 tattgtcctt attgcgcagt gaataaagtt tctgtggcgg atcgtaacgc ttccctcctg
     6241 ccgcctgata cacacccgat accacccgat aagcagaagc cctgtccatc tgtgagggtg
     6301 gcagagtgcg taacagttgc caggtatccg cgtaacggga gggcattctg ccttccatcc
     6361 attctgcgag gcgtttcgcg ctgaccgttc cgttcagcat ttccgataca ctgtgtcgta
     6421 ctttttttat gctctccccc cagcctgccg tattgtgttt cgtcttcgcc ggaatatcgc
     6481 tacgatacag cgctatcatt gccagggtat cagcatcagc accttcgttc gccagttttg
     6541 cgtagtccgg tttcgggaac agtttgctca gcggctgcgt ggcataatcc ctgtcttcca
     6601 gcgttttacc cagtgtttca gcaagctgtg cataacggtg ttttgccgcg cccttaattt
     6661 cttcaccaaa gtcttcaatt tttttgcccc tgacttcacc tccgcgcacg gtagcttccc
     6721 tgtcagtaga gacagttgtt tcagattcag gtgcgattgt ctcaggctgc gggatgtcac
     6781 gatttcctcc ggcctgtcgg cgtacttctg cctgctgtgc aagctcaggc agtgagtccc
     6841 gtacagtgcg gggaagtgat tcaggctgag gcatacgaac attacgcgca atgtcaggag
     6901 ccggtagccc ttcacgtacc ggtgctgtcg cgttctgcgc atccggtgac ggcagaccac
     6961 gacgaaccat gtcaccctcg agtgtttccc cctggcgacc agcggcgttt tccggtgccg
     7021 gagcttgtcc tttctggaaa ctctggccct taacctcacc ggttgtggta aaacgaccac
     7081 cacgtccggc ctgattttca tccggcgtac gcgccacttc tcccggtaac ggatatccct
     7141 gtccgggatg aatatcgccg ggagcgggca ggcgtggacg ctcagttaac tcctgtgctg
     7201 tcggaccggc atcaccttca gccatctgtg aacgtacaag ctcctctgcc gtcggcgcgt
     7261 tgccacgggc aaggcgggcc tgtacctccg tatcatcccc cgtgaaacca cggaatcgcg
     7321 ggtcacgcat gaacgcgggc tgctccatcg ggtcagcatc agcaatacgc tctccgtcag
     7381 ccagggtgtt aatggtttcc gttgccacat cttcagagaa cgcaggatta tccatttcaa
     7441 cggcatactg attctcgcct ttcttcacta cggacggttt caggccggtg gcggcggcat
     7501 tacggaaaac atcactcccc atggcgcttt tctcatccgt gaaataacgg ttgtccgggc
     7561 gcaccttctc aatgcgttcc gctggtctgg acgcaccatt cccgttaatt tcaatgcgga
     7621 cttcattcgg gtcccggaac cgcaccgtcg ggtaaatgct cccgttacca tcctccgcat
     7681 tttcaggctg tgactgtgct tccggcgtca cttcctgttg tgggcgaatg acttctcgta
     7741 ttgcctgaaa ctgttgttct tcagtctcgg tgcgcccctc tttctggctg agtctgcggt
     7801 attcctcaag cagttcagaa cgcggcttcg ccttcagctc attcatcaca gcctgtcgct
     7861 tcgtctgttc gtccagttca ttcaacaact ggctggcagc ttcccggcga tgagctgcgg
     7921 atgcgtcacc ctctgttgcc atatccgcat cagcatactg ctccagaagc tgctcgcgat
     7981 tcatgccctg catggattca cgctgctgcg ccaccggatc aacagattct ggttgcgggg
     8041 ctgcgtcgtc ctgctgcacg gtttcagcat cacgcatggc ggtttcttct gcggcctgac
     8101 gtctgccacg atatccggca accgcaccga atggagcccc catcgcagca ccaaaggctg
     8161 caccttcgat cgttgcgtca gccacgccct cccacggtga cacacccatt ccggcggtct
     8221 cacgcaatgc cgtgttttcc tgatagcgtg aatagccgcc ctgcgccgca ttaatcgccc
     8281 cctgttccgt ggcatttctg acaatgccgc ttttaacggt cttcgctgtg cctcgtgtca
     8341 ccagattaaa cagttgtgcg tcacccagtt ttgccgccat ggcattcaca gccagcaatt
     8401 caggatcggt tgccagctgc gcgcgcacct catcggcaac acgctctttt gccagatcca
     8461 ttttctggcg atcagtaagc tgtgcgtgct gcgggtcggc gtcaatggac aaaaacgtct
     8521 gctgaaattt cggtgactgc gccagctcag agtaatcagc attaagaaca gcatctgctg
     8581 ccgccattgc actctgcccc tgtgcactta cagtggaatg ggtgatcagg cccgcctgga
     8641 ataaatccgg cattttttta tcgacagctt ctgctgccag tgccgtggct ctttccggct
     8701 gcatccctgc cgcgatgtat tttttctcca gcccggcggt cagcattttt cgcagtgtga
     8761 catcacccac ctttctagcc acaccgccag caaccatatc aggtacaagc gcaccaatca
     8821 ggtttacacc cttcgccacc cagaccgcag aatcattata gccttcggtc atcggcgtat
     8881 tcagcgcacg aactgcaccc ggcgacatct taccggtcag ccattcatca gtggctttgg
     8941 caccatcact gacagcctta ccggttgcct tcagaccttt accgacaata tcagtaaccg
     9001 cgtttttgcc atcaggcagg gtatcgatga cctcatcagc ccccctgctg ccaccggcaa
     9061 aaatatcctg cacagttgcg acacccggca gccccatacg gctgaactca tttaaaatac
     9121 gcgcccctgt ttttaccggg ctctgaatca tcgcatcacc gagtccacgg gccatttccc
     9181 ctgttccccg gacggactgg gcgaaacctt tacccattgt tggcaataca tcgcccaggc
     9241 tgaacgacgt actgttatcc ttccagcgat tcggatcaga gaaaaatgct tcatagctgt
     9301 cagtttcgcc gggttgctga atgttcaggc tgttacgatt ctggttaccg agttgcgcct
     9361 caggacgctg ttcctctgaa taggccatac agactccata aaaaaacccg gcacaatggc
     9421 cgggcatcag gaaatggtat taatggaata tcgtgaatta ctgggcgtaa ttctgtttca
     9481 gtccacggat aaactgagat gcagatgaag cactttcatc tctggcctgt tcccccctgg
     9541 ccttttgaat acggagaaaa ttcgcatacc cgttctccag caatttctga ttctgaggtt
     9601 ccagcatttc aggctgacgg gccgctacat ttctggcaaa tgacagcttg tcaggatcat
     9661 ctcccgccca gttgataacc tgttgctgaa gtttttgttg ctgaattttc tgttgatgcg
     9721 gttgtgaacc agtcgcagca taatattcat ccaccgcagc ctgagcatta ccgccattct
     9781 tgatcgcgtc cgccgctacg ttgctggccc ccttctgaag ctctttcaga gaaagtccct
     9841 gctgcttcgg cataaaataa ccataatttt tcgatatctc tgcaaatttg ctgcgatccc
     9901 tgacctgagc gatagcctta tcaacgggta tcgccagcac ggtttgatca tcagggtgcg
     9961 cactgccgta ttctgttaca ggtttatgcg cggtggagcc atcgctgtat gtgagatcaa
    10021 ggccaataag tacgtaccct tcctgctgcg ctggtacgat actgccaatc ctggcctctt
    10081 ttatcgtttt gtccccggtt gaatcaggca tgccaatacg ctgttgtagt tccggggcaa
    10141 acacgccgga aagcacatcg agattttccg gggtattcag cgaatcgatc gccatgtccg
    10201 gcttatcacc aaagattttt tgcaggttga tcacggcctg acccgctttc gctgcatagc
    10261 cttcgtttga cataagaatt aatggatgtc cctttggcag ctgcccgaac agacgcgttg
    10321 cagcctcctg atcgccagcc tcaattgctt ttccaagcgc ttccattagt ggctggctat
    10381 gggcaatcat atcgttatac tcaagccgtc gctggttgta tttctgcaac tggagtcgct
    10441 gctgctccat acccagtgac gcattccggt aattctggtt agcgttaaac tccctctcct
    10501 tcagtgcata attacggtcg tcaactttgg ctctgtaatc aaagttccgc tgatcaacgt
    10561 ttttattatg ttcaaactga gactgcgcaa actcaaaatc gcgctcgtta tttttctgtt
    10621 gccgggcaag ctgtgcttca cgcagaccaa gctccttacg acgggtcatt gcctggtcaa
    10681 cggtgctgaa tccggcaagt aacccctgtg caaatccgct cattcaccac tccttaaaac
    10741 aaagaaccag caatgccgcc aattactgct ccagcaacag caccaacagg accaccaacg
    10801 gatgcgccaa tagccgcccc cgtaccaatg cccgtaccga tattctgctt gttctgcgct
    10861 ttctgttgcg ccgccatctg tttgttcgca gcctcaattt cttcacgtcg tctgtctgcg
    10921 tcacttattc cctgtaatgc ctcacgccgc gactgatttg caatatccag taaaccgtat
    10981 cccatattgc ccccttacgc tgccaccagt tggccgccaa cactcagttt ctgtcttgcc
    11041 ggtgcagaag cccccgtcag aatattcatc tggcgatcct gttcggcttc acggatacca
    11101 tttttcgcgc cagcaattgc cagggcatta cgtaacccca gcgtattact gtcgggatta
    11161 tccgggcggt ttaccccgta tctcgccatc tggttatcct gcgccatctg cgctgtacgg
    11221 agactggaag tggcaaggcc tcccacccgt gcaagctgtg cattcatcag actgttgttc
    11281 tccccaaggt cagccagcct tgccacgcgg ggcaaatatc tggttcgcca gtcgtcgtat
    11341 tgttggcgtg tcagcgctgc tgacgtctgc cagtcaccct gtgggcggtc tgctcctgaa
    11401 taaccagccc ttgcgagagt ttcgtatttg ccatactcca taattacagt ctccagttct
    11461 gagcctgatg ctgaatggca ttagcgccgg tgccaggtgt tttagcaccg ccacttcctc
    11521 cgctaccgcc agccttatgc atcgcatatg cacctgccgc acccagacca gcgccaacaa
    11581 gagaggctcg cccctgctgt ttcgtaaacg ccgcctgtgc atccgattta gcttttgcca
    11641 gactgctgtc tgccagagag ttaaaactct gtaacgcatc cgccttctga ccagaaccga
    11701 gagcagcaac atcctgtagc ccggcaacat acttatctgc ctgcgatacc tgcccccgtg
    11761 tggttgtgtc aatctgcccg gttacctggt cactctggtt cgcattcatt accgcattaa
    11821 aacgaccact ggatgggtca acgccggact gagcaagatt acccgcaagc tccttccgtg
    11881 cttcgccaaa ctgtttctga taccccagat ttgtggtgcc agcaatattg tcgtactgct
    11941 gctcactgtt caggtcatcg accttttcca tgaagttatc ttcagccggg cggaggatat
    12001 ttttgtaatc ctgccaccct ttccaggcca cttcttcctg tgctatttgc gctgctgttg
    12061 gttttacttt ggtatcacca ccgcctttac ttccacccat aatggccccc tggataacaa
    12121 aaaaccctgc cggagcaggg tcagaatgtg aattacaatg ttggtttaaa cacaatgatg
    12181 aaaactgtag cagtcagacc gctatcctga acaccatgaa gccatcctca tcatccggca
    12241 ttcgctcaaa gcccagtcgt tttcccagcc ggataaatcc ccgccttgcc gtatggaact
    12301 cagcccagcg tccgccagcc agatgggtta atgtcttcac ctccggcagg taacgctcaa
    12361 cgctgttact ccccgtacac acgcccagca acaccagaac ataagggata ccatcagcac
    12421 tgagcacaga acgcagcacc aaaaagccat caggtgcctc aaaacaaaac gcctgctttt
    12481 taaggcaggc gtctttaact tcattcataa attcagggtt gcgggaattt ctcacaacac
    12541 gctgcatata ccagagaatt ttatcgttca ttctctcacc tgaaacgggt gccatatcgg
    12601 ctgaacctca gcaaccagtt gacggggact ctcgtccccg tcgcggtttt cctactgctt
    12661 acactgtaag aacgccgcaa actccgctcc ccacaaattc agccggaact cacacagtga
    12721 accgtgcaac atccagatgg tgaagataac cgtcatgcaa attgtgacgg tgataagcga
    12781 tttttgcgac atagcgcttg actcctttta cagagaggcg ctaaccttct acttgcttaa
    12841 ggtacgaagt gttagggcct cgggttaacg ttaagttgac tcggggcctt tccacatcag
    12901 gccttcaggt tcaccctcca gccatcagcc gaaaggcacc cacgcataat ttacggtttt
    12961 tgccctgtac gggcaataaa aaacccgcca tcacagcggg tagtaaacgg ttaccggata
    13021 caggtcagaa tttcaggcca atgccagcag taacgccact cgtgcgccag tcgccagagc
    13081 cggaaccttc gtaagccaca tccagtgtaa cactctcatt caggttaaac tgtgcacctg
    13141 cagcccacgc aagggaggtt tttttcgtgc tgttgctttc agaaaatccg ccactactgt
    13201 taatattgtc cttaattttc aggtcagcgc taactttagc aacgcccatc cctgccatcg
    13261 catataaact catgtactga ttaacccgcc atgaaggccc cgctaacaga ctccagtaat
    13321 tcgccctgat atccgttctg gcagaagctg ccggattctt aatcttcctg gtatgatcgg
    13381 ctgactgcac atcaataaat gtctgtgagt tagtgagaga acgcgtccat gtaaaagagg
    13441 tgataacgcc aaaatcatcc gttatctcgt agcgatacct gatattaatg ccctgtggat
    13501 ttttatcctt gccttcatat ccactgaccc gaccatctgt atattcgccc aatgaagaaa
    13561 agtagtttct gttgacgaaa tgactgaaag tctccctgtt atgagcagtc gcatccttta
    13621 caaaatcctt cagtcccgga aactgaaagt gcgcataccc cagagaaatt gtatgctctc
    13681 cttctgctgc atgtgcagat aaatttacac aggcaatccc ggagattgca cacacaacca
    13741 gtgttgctat actcttcatt taagatatcc tgtattcaga taacacgcta aactttttaa
    13801 taatacatct ttttagacat tttaaatgaa tat
//
LOCUS       DAZFKY010000126        13710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFKY010000126 DAZFKY010000000
VERSION     DAZFKY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            64..561
                     /gene="ftnA"
                     /locus_tag="ACHKCL_003303"
     CDS             64..561
                     /gene="ftnA"
                     /locus_tag="ACHKCL_003303"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5764202.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(598..837)
                     /locus_tag="ACHKCL_003304"
     CDS             complement(598..837)
                     /locus_tag="ACHKCL_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5764203.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1029..2240
                     /gene="tyrP"
                     /locus_tag="ACHKCL_003305"
     CDS             1029..2240
                     /gene="tyrP"
                     /locus_tag="ACHKCL_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5764204.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2302..2967)
                     /gene="yecA"
                     /locus_tag="ACHKCL_003306"
     CDS             complement(2302..2967)
                     /gene="yecA"
                     /locus_tag="ACHKCL_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5764205.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3324..4325)
                     /locus_tag="ACHKCL_003307"
     CDS             complement(3324..4325)
                     /locus_tag="ACHKCL_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5764206.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(4331..4678)
                     /locus_tag="ACHKCL_003308"
     CDS             complement(4331..4678)
                     /locus_tag="ACHKCL_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO5764207.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(4708..5358)
                     /locus_tag="ACHKCL_003309"
     CDS             complement(4708..5358)
                     /locus_tag="ACHKCL_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764208.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(5374..5778)
                     /locus_tag="ACHKCL_003310"
     CDS             complement(5374..5778)
                     /locus_tag="ACHKCL_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5764209.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(5868..6005)
                     /locus_tag="ACHKCL_003311"
     CDS             complement(5868..6005)
                     /locus_tag="ACHKCL_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764210.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            6077..6280
                     /locus_tag="ACHKCL_003312"
     CDS             6077..6280
                     /locus_tag="ACHKCL_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5764211.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            6302..6652
                     /locus_tag="ACHKCL_003313"
     CDS             6302..6652
                     /locus_tag="ACHKCL_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5764212.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            6663..6941
                     /locus_tag="ACHKCL_003314"
     CDS             6663..6941
                     /locus_tag="ACHKCL_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764213.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6953..7195
                     /locus_tag="ACHKCL_003315"
     CDS             6953..7195
                     /locus_tag="ACHKCL_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5764214.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            7192..7305
                     /locus_tag="ACHKCL_003316"
     CDS             7192..7305
                     /locus_tag="ACHKCL_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764215.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            7398..7814
                     /locus_tag="ACHKCL_003317"
     CDS             7398..7814
                     /locus_tag="ACHKCL_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764216.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            7838..8041
                     /locus_tag="ACHKCL_003318"
     CDS             7838..8041
                     /locus_tag="ACHKCL_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764217.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            8038..8304
                     /locus_tag="ACHKCL_003319"
     CDS             8038..8304
                     /locus_tag="ACHKCL_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5764218.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            8301..8600
                     /locus_tag="ACHKCL_003320"
     CDS             8301..8600
                     /locus_tag="ACHKCL_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764219.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            8923..9153
                     /locus_tag="ACHKCL_003321"
     CDS             8923..9153
                     /locus_tag="ACHKCL_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO5764220.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            9226..9591
                     /locus_tag="ACHKCL_003322"
     CDS             9226..9591
                     /locus_tag="ACHKCL_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764221.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            9598..12420
                     /locus_tag="ACHKCL_003323"
     CDS             9598..12420
                     /locus_tag="ACHKCL_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5764222.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            12497..13456
                     /locus_tag="ACHKCL_003324"
     CDS             12497..13456
                     /locus_tag="ACHKCL_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5764223.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKAPADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            13461..>13710
                     /locus_tag="ACHKCL_003325"
     CDS             13461..>13710
                     /locus_tag="ACHKCL_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5764224.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQ"
BASE COUNT         3664 a         3622 c         3193 g         3231 t
ORIGIN      
        1 ataataaaat aacaaattat agtgacgcca cagcaacaaa tataaccttt gtggagcact
       61 atcatgctga aaccagaaat gattgaaaaa cttaatgagc agatgaacct ggaactgtac
      121 tcttcactgc tttatcagca aatgagcgcc tggtgcagct atcatacctt cgaaggtgct
      181 gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg tctgtttgat
      241 tacctgactg ataccggcaa tttaccgcgt attaataccg ttgaatctcc gtttgctgaa
      301 tattcctcac ttgatgaatt attccaggaa acctataaac acgaacaatt aatcacccag
      361 aaaattaacg aactggctca tgctgcaatg accaatcagg actacccaac atttaatttc
      421 ctgcagtggt atgtttctga gcagcatgaa gaagagaaac tgttcaaatc gattattgat
      481 aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa agaactctct
      541 accctcgaca cacaaaacta atgctctccg gcggcaggtt tctgccgcca tttcaaatca
      601 gtgacggcaa atcttactct gatcggtcga aaaaccttct tctgaaggta taaatttgcc
      661 ttttgctgcc agaaacgcta ccagctctcc cgctgtcatc ccttctgccg agcaagtgtg
      721 aaaacgtgcc tgttcaccaa aacgcgcttt aatcgcagct tccagactgg catgcgtata
      781 ttgctcgcct gattcaatca tcatatttaa cacttcatga ccgtgaatag agtccatcgt
      841 tcctcctcaa aaaaaagcct agcgtagcga ttgccgctta tgaagacttt gcgccagcgc
      901 aggactgaat gctttttatt gtacatttat gtttacacca tatgtaacgt cggtttgacg
      961 aagcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc gtcaggacag
     1021 aagaaagcgt gaaaaacaga accctgggaa gtgtttttat cgtggcggga accacaattg
     1081 gcgcaggcat gctggcaatg ccgctggctg cggccggtgt tggttttagc gttacgttaa
     1141 tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg ctggaggtgt
     1201 accagcatgt tccggcagat accggtctgg gcacgctggc aaaacgctat ctgggacgct
     1261 acggtcaatg gctgacgggc ttcagtatga tgttcttaat gtatgctctg actgcggcat
     1321 acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca ggtatttcaa
     1381 tgtcggcaac cgctggcgtg ttgttgttca cttttgttgc cggtggcgtg gtttgtgtcg
     1441 ggacatcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt atttttctgg
     1501 tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta accctgccgt
     1561 tgcaacaggg gctggcactg tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg
     1621 gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta cgctgggtgt
     1681 ttataaccgg tagtgcgatc cccctggtgg catatatttt ctggcaggtg gcgacgcttg
     1741 gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgccgga ttaaacgggc
     1801 tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggca gtgcatttat
     1861 ttgctgattt agcccttgcc acgtcatttc tcggcgttgc gttaggctta tttgattatc
     1921 tggcagattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact ggagcaatta
     1981 cctttctgcc accgttggcg tttgcactgt tttatccacg aggatttgtg atggcgctgg
     2041 gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcgctg ttgacctggc
     2101 aaagcagaaa gcacaatcct caagcgggtt accgggtcaa aggtggtcgt ccggcgctgg
     2161 tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt gcggcagggt
     2221 tgttaccaga agtggggtga ttagatagcc tcaaattcct tattgggtgc cagaattaac
     2281 gctgacaccc aatttgactt cttaatgcag gcagcactgc ttaaattttt taccactacc
     2341 gcacgggcaa ggatcgttac gccccggttt ctcttctgct ttgatcggtt gctgaacagc
     2401 tttttcctgc ggatgcgcca tccagtacgc atgtagatca agcgccgcca gtcgaatggc
     2461 atctacgctc tcttcaaacg cttctggcga catcttctct acccgctcga agttttcctc
     2521 agtaccgtgc agcgctatcg cctccagcgc tggttttaac gaatcgggca atgttgacca
     2581 gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc
     2641 gctgccatca acttctcgca agccgaataa cggctcaaac tgctccggga attcgttcag
     2701 acgctctgcg gtatcggcca tatgttgaaa agccaggttc ataaagcgcg tcatctcttt
     2761 ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca gccactgtgc
     2821 cggttcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca gctccgccac
     2881 atcaaggatg gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc attccaactc
     2941 actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa ctcgccagca
     3001 cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc cggtgattaa
     3061 aaatcaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga aggtggtttc
     3121 acgacactgc ttattgcttt gattttattc ttatctttcc catggtaccc ggagcgggac
     3181 ttgaacccgc acagtgcgaa cgccgaggga ttttaaaaac tatcaaccac catttataaa
     3241 tcataacctt atgattttat taactttgaa aatggctcta tacaacgcca tttgaatcta
     3301 ttgtcacttt ttatcgccac tttttaccct ctattgtcaa aagggttcaa ttcaacagca
     3361 gcctctaaat gacctggagc aaaatgcgca taacgcatag tcatttttat atcgctatgc
     3421 cccagtattt tttgcaacac aagaatattt ccaccccgca tcataaaatg actggcaaac
     3481 gtgtgacgta gcacatgagt taattgccca tcaggaagct cgatcttcgc tctcttaatt
     3541 gcagcgtcaa aagcctcata acatggtgaa aatagcgctc ctcgtttttt aggaagcata
     3601 gcttgcaatt gaggtgaaat cggtacagtg cggttcttct ttcctttagt tttaacaaat
     3661 gtgattcgac cgggcagtac ttgagattgc tttaatcctt ctgcttcact ccaccgagca
     3721 ccagtcgcaa gcccaatacg gacaacaacc cccaaatctt tattccgtga ctcatcacac
     3781 gcaatcagaa ggcgttcaat ctcatctaca tacagaaacg ccagttcctt ttcttcctca
     3841 cgaaacttgc gaataccagt cagggggttt tcaccagacc actccccaag gcgcttcagt
     3901 tcggcaaaaa cagcatgtag atatgactgc tcgcgattaa cggttgcttc actaagtttt
     3961 ttcttcccct tgggattcca ttctcctgat agccttcttt cccgataagt agcaaacata
     4021 tttttgtcaa actgagaagc aaatggatct cccagcctgg aacaaatcgc ctcaagtttg
     4081 actttgcgct ctgcaccaga ggacaaggtt ttaccgtaca tctcaaacca acgagcaatc
     4141 aactcagaaa gacgaggacc agaaccatct tgaaactcgt ctccaactct actattcatt
     4201 aaacggcgct cataagagag cgcctcactt tttgtcgcaa actgtttacg aatgcgtttt
     4261 cccgatgccc cgtagggata acattcacaa agccatttac ctgatggaat cttacgaacc
     4321 gacattttag ttacttatca cataaatcaa atgcagcctt agtgacatcc cccagactct
     4381 tttttaaccc tggggcggca tcattatcta gccaaaatgg attattgtta tctaacggta
     4441 acgcaccaaa tgttttacct tttattcgag ccaaacctgt aagtgcatat aacttattat
     4501 cgtcaaaatt catcacataa ggattaccat caagacactg taattgaacc tcatcagtat
     4561 taaatggcca taccccattg aaactctcac gttcaatagt tttaaaaggc attgcgacgg
     4621 cggaaaaaga aaacatagat aaaaaagtaa ctaatagttg aacctttttt actttcatat
     4681 cattacctca atttagctca agtaagatta caaattaaaa aacgccctag aaatgacacc
     4741 gcctaccaaa acccctacgc agataaagaa tattatttct tttggataaa gtcggattaa
     4801 ttctgaagca cgaagtcgga cttctggtaa ggtcgaactc tctatgtggc ttgatgccga
     4861 ttgttgctct aaccacgaca atgcagactg taactgagaa cgagtaagat cgtttaaacg
     4921 tcctgtaccg aaattgatat ggcaataccg cagaagtttt tgtcgaagtc cacagtcttc
     4981 actgttacgt agtaataaac ttacaagagc cttacaggca tcatgatctt tacatcgctc
     5041 aagcattgca tgcagaaaac tctccgctgt tttatattga tttactgtca tatcatcaat
     5101 accagctaca ccaatctccg catgtacttt ttgccaaata ataaacgctt cagtattgct
     5161 agcttctgca atagcagcaa ccaagctatt tagctcctta cgctgagcct taagcaaagg
     5221 gcgatcgtca tcatcattat tcgaagggat tgcgatattg acggtatgag aaccatcata
     5281 tcgctctatc tgaatattct tttcgtgaaa atcacgccca gcaacgcgat tgtttgaacc
     5341 gtttgagttg acggccatgt cacctcccta ctatcaccta cccttagttt cgttatagtc
     5401 acgaccagcg atacggttat taccaccaga aatatttaac tcacgtcctg atggctgagt
     5461 ttccttttca ctgatcgcac cttttaaagc cccaatcacc gcgtttttca catctaacga
     5521 agctgctcga aagcgagtaa tcaattcctg ctcatcatcg ttataagttt caggtgagtg
     5581 aattcccaac acaacatact gaacatcaag gccaaaacga gacagcgctg ccaaatacgc
     5641 agcatcagga aagctatctc ctttctcata tctaagctga gttagctttt tgactccacc
     5701 aatgtcgctc atggcaactt gactaagtcc caatctttcc ctttcctcac gcaaccgccg
     5761 accaatatca tttttcatac aaaaaccttg acaggtatct tttttgatac caaaatgatt
     5821 tcacgagcta ttagatgatc acaatatacc actatgaaac aagttcttca cgataccaga
     5881 tcacgcattc cgcgtaacac cgccacaggt ccaagactgg cacttcggct gtccctcgag
     5941 gagcgagccg tcattgatga aatggcagct aaagaacaac gctcatcctc taacatggcg
     6001 cgcatgatct cccttcgcgg cctagagcta acccagaaag aacaaaacaa atcttcctga
     6061 tcaggaggct agtgggatgt caggtataac catcaatatc aatgtgaatg ccccctatgt
     6121 atccctgcag aaatatgcag agataacagg tatccctctt aatacatgca aaaagatgtt
     6181 ggctgacggt cgaattatta tccgccccaa acgcgccaaa atggaaaagc ctgaagtaaa
     6241 ccttgtggcg atgttaaaag acgctttggc taacagctaa aacaatgaac agagcaccat
     6301 catgaaaaaa aacgctaata atccatactc caaatttcgt aatggcgtag aacgccatgt
     6361 acaccacgtc gctaccagtg catcacgtag taacagtcgc tataacctga acgagacgca
     6421 tgcaacaccg gatggccacg ctgtaaaaca aatcggcgaa catgcctggc tgattgagaa
     6481 agctggaatc gtggtccaca aatgcccacg caatccgttt accgggaacc gcatttttgc
     6541 attgaactgc ggcgacaatc acttcgggca ggatttcaca ttatacgaag cacttcgcac
     6601 ggttgatcgt ctgcttcgcg ggcaaagttt tattaaacag gctgatttat aacaggtgct
     6661 ttatgaccaa agagcatgca caaggtgtat ttatccgttt tattgatttt cgcggtgaac
     6721 tgttattacg cgcatcagcc attgacggaa ttgttccatc cgaaaaaaac gcagctactt
     6781 acgtttatct gaacggtacg cgcctgactg tggagcttcc gtaccagacc gtacgagaaa
     6841 tcattagcga agctgaaaag gctcgtcagg ttaatggcga tgaaccctat atcgaaatta
     6901 tttgtatgga ttcagaagct gaaattcaga aagcagatta aagggcgttg cgatgggcaa
     6961 agaatataaa actctcatta acaaagcacc tgaacgattt tattttcgct taagtgcatc
     7021 aggcgctcat gctgaacgtg cagcccgtga ctcattgacc agggcaatcc ggagtttgta
     7081 tgacgttgct ttttacgctg atgatctgga tgcacttaac gaactttccg agctgatctg
     7141 tgccgcagaa tgcggggaac acattgaacc atataagctg gggaatatcg catgagtata
     7201 tttatctcat ggcttgttct gattatttcg gtggcctgcg ccattgggat tatgcgaatt
     7261 attcattcag taaaaaagat tgaacgcttt ttcactgaca aataacagct taaacagaac
     7321 accagattaa acccgaaaac ctgaaaacta tccgcattcg cggaggtatt cgcacacgta
     7381 aataacggag atataaaatg aacgcaaaag aaaaaaatat tatcaataca ttaaaaatag
     7441 tgtcagcaga gcaagacaag ctgtccaggg cagcccagaa agataatcaa catatggcag
     7501 cgctttacgc actgaccatc gcaatagcca cccctgaagc agccaaagtt attgaggaac
     7561 agagcaaaga aatcgacact cttaaaacac agtcaacagt tgcagccatg aatccgtcca
     7621 gcattggacg ctgcatttat attcttggtt cagcaatgat gctgcaatac accattattg
     7681 ccgaactgca cggcaaatac ctgataacgc cttaccacac aaaagagtca gagcttctga
     7741 caaatctccg cctgatagaa cgctctcaag ctgtattcat tgatgacgcg caacgtgccg
     7801 tatttaacgc atagggttac tggacaaagg gggcgcaatg gcaattaagc attttcccgt
     7861 cgttcgcttt acctccagag ggcgcgaata cgaggtcgac gaacgcctga ttaccactat
     7921 cgacaaacat cgttcggaaa aggatgcaca ccacatctac ctcactgacg gcacttactt
     7981 ctgcgccacc aacgtggcgc gagtaaatct tatccgacag gtacaggagc cacgcagatg
     8041 accattctgg actacatcgc tactcatccg gggtgtagcg gcggagagat cgccgcagca
     8101 ctgaataccc caaccacagc cattaatgct gagttacgcc gactttggcg cggcggctta
     8161 gtcatcagaa caaaccgcag cacaggtggt cgcgctcgca aaacaggagg ccaggcttct
     8221 taccacgtaa acccgatgcc gttcgggtgt agcaatccac ttactcacat gtttaaccag
     8281 ctactgaagg aagccagagc atgagcacca tcaaccacca gaagctacgc gaactggcat
     8341 ttgccctgca acgaatggca acgcctcaaa aattactggc atttcgcgca atgctctcgc
     8401 cgtctgctgt gctggcactg ctggatcagc tggagcacgc cagaaccacg gctcctgcca
     8461 ttcgcctcac gctccatcat gaaatcgctg atttctgcgc aacgctgggg gcacctggcg
     8521 aaccggaaac gccggaagca atgcagcaag agctgctgca acgcatcgat aacgtttttg
     8581 atttttttct gaaccagtaa gaaaccagaa catgcacaca caaaaaaacc gcttgccatg
     8641 ccgcaatcgg tcaggttaca tttccgctgc acctcataaa acgggtgccg ggattctcaa
     8701 cccgatacag agcaaagcgc ataaccgcgc tagcggtttt tttgtgcgta ctgtattgcc
     8761 acgtcttttt cgcgtcagaa ttatggcggg gcgtacgggg ccgacttcgg tcgggccgga
     8821 ttctttgctc tccggtgttg agaaccctgt acgtctcgcc agcccgagat tctcaactct
     8881 ggatggtgag ttatttctat caccgagcaa agaggccaca ccatggcaga ccgcaaacag
     8941 caccgcgcta tcgcggagcg tcgtcacatc cagactgaaa tcaaccgcag actttcccgc
     9001 gcatcacgcg tcgcgcaaat catgcacatc aatatgctgc atgagcgcag ccacgcacta
     9061 tcaaacattt attccgcctc tgttttcagc tatctggcgg atgatctgca cgagtttcaa
     9121 cagctcatcc agcagcaaaa caaactccat taattcctgt tccgggcctt tcctgcacct
     9181 tgcggcggga ggccttcgca catctgtaac aagaggattg ccgcaatgat tctcgccaac
     9241 gactttcttg aatacctgct caacacagaa cgtgatcttg ccgctcgcgt gcgtgatcgt
     9301 tatgacatgt acctgaaatc cctgcctgta ccgcagctcg ctgacggaaa gattgttatt
     9361 gatggtcgct acatgattga cagccacgag ggaaattaca ggctttaccg cattgaaggt
     9421 ggcaccccgt ccgttattgg catttaccag cgcccatcct ctgcaatcgt cgatgtgatt
     9481 gccgacagca tccgcatcac acatcgccat gccgacacag aagacaccgt gctggaaatt
     9541 cagcggctgg ctacagcctg ccgcgacacc ctgaatggca tgacgaagta aatcactatg
     9601 acggcagagt acatcaggga ctggcaacaa ccgcgccacg cagtggggcg tgaaggaacg
     9661 gggatccccg ctcctgaatc cgcgctttcc tcctggctgg atgcctaccg ggtagagaac
     9721 gagcgccgcc aggaaatggc tgatgcggcg ttctccgcaa cgccgctggg caacctgatt
     9781 aataaaagcc tggacgcaca ggaaaaacag gacaaaacca tcacactggc aggagacgcc
     9841 agaaaacagg cacgcggtgc ggtggatgaa gccatggcct cgctgcgcct gctgccgtcc
     9901 tatctgcgcg atccgcttat tcgccacctc tccttcctgc gcaaaaaaca ggaagccgat
     9961 cgtcagaaag gcaaaaagag ctggcaggct gaacgctacg cgcgcggaaa cctgcgcaaa
    10021 atattcgaac gtctggagcg caccgatcac cgctggctga cacagggtta tcgctccctt
    10081 gccggacgcg aacgcctgga cgatttgctt tacctgccgc agctcaacaa acaccagata
    10141 cagacgctgg ccaccatgac ggcggcgatg ttcagcagca ccttcgaaaa actctgcgat
    10201 ggctttggcg cgaccgatgg cgaactgacc atggatgtaa cgctgaaggc gtatcagatg
    10261 ctggcccgca tggcgttaca cctgcacgcc atgcctccac attatgacgc actgacaaca
    10321 gacaaagacc ggaggaacga accggacacg gagctgctgc cgggcgcaat ccttcgcctg
    10381 acctgtgcgg aatggtggaa acgcaaactg tggctgttac gttgcgagtg gagagaagaa
    10441 caactccgcg ccgcctgtct ggtttccaga aaaacatcgc cctatctgag ccaggacgcg
    10501 ttaagcgagt ttcgcgcaca gcgcgagaaa acacgcgatt tcctgaaaag tttcatgctg
    10561 gaaaacgaag acgggttcac gattgatctc gagacagtgt attacgcggg agtaagtaac
    10621 ccggttcacc gtaaggcaga aatgatggcc accatgaagg ggctggaact tctggccgaa
    10681 gcccgtggcg acaaagcggt gtttctgact gtcacctgcc cgtcaaaata ccacgctaca
    10741 acagagaacg gtcatccgaa tcccaaatgg aacggggcca ccatgcgcga ctccagcgat
    10801 tacctggtta acacgttttt tgcggcggtc cgcaagaaac tgaaccgcga cggcctgcgc
    10861 tggtatggca tccgcacggt ggagcctcac catgacggca ccgtgcactg gcatatgatg
    10921 gtctttgctc atccggaaga aatcgacacc attgtgtccc acacccgcga tattgccatt
    10981 caggaagatc gtcacgagct gggcgatgat attactccgc gctttaaggc ggagtatgtc
    11041 gacggctcaa aaggcacgcc aaccagctac atcgccacct acatcggaaa aaacctggac
    11101 agccgcgccg tggatggcat cgacccgaaa acaggcaaac cacgcgttga ccacgaaacc
    11161 ggaaaatcaa tgaccgagag cgtggaacgc gccattggct gggcgcgcct tcaccgggtc
    11221 cgccagttcc agttctttgg catcccctcc cgtcaggtgt ggcgtgaact gcgtcgcctt
    11281 gccagccaga tggcacgcaa cccggaaggc ccgcaacggc tgaaggatga cgcaatggat
    11341 gcggttcttg ctgccgctga tgccggatgt tttaccacct acattgagaa acagggaggc
    11401 gtacttgttc cacgcaagga ctacctgatt cgcaccgcct acgacctcgc agatgagctg
    11461 aacgattacg gcgaacagag cgtacagatt tacgggatct ggtcaccact catcggggaa
    11521 tcctcccgtg tgtgcacgca cccggataac tggaagctgg taagacgtaa accgggagta
    11581 gaagacagcg cccgcgaaaa tggttttgac cttcagggcg gccctgccgc cccttggact
    11641 cgtggcaata actgtccccg tgtacaggaa acggacaaca acgggacaga acagccggaa
    11701 gaacggccag caccgtggcc gcagcttcct gacggcgttg acgtgaacga atggatgcgc
    11761 tcactgaaac ggcacgaacg ccgggcgctg atgcgttcgc ttcgtgacaa acaggcaaaa
    11821 aacagcagtg atgaaatgca gagctggaca cagagccgca aacagcagcg gcctttgcct
    11881 gataaccacg aattactcgc taaagaatgg cgggagtctg ctgaatctct cggcctgcat
    11941 atcggtgaac aacagatgca gcacctgtta cggggcggca gtctgtacgt tgacggcagc
    12001 atcattgcac cgcagggatt tgaaattgta cgcaaaccgg atacccgccc ggacagccga
    12061 atcacgcagc tctggcagcg cctgagccgt aatcatggcg taagcagcac ggagatccgc
    12121 cataacccgg tcgccagcta tctggcacag ctgggggcat cagaccctga agccgccgca
    12181 cgcctggcat ccacacttca gcaggaccag aacaccatga aaacacccgt taccgtgctt
    12241 tctgacatgc tgcgcgccat ccgcgacgca gagcacgcac agagaatcag tgaaaccact
    12301 gaacgcgcca gccgcaaagc agacctgctg cggggtggcc tgaccagtgg aaacaaaaaa
    12361 cagacagaaa cgggactcac aaatcccgta aatgagcaaa aaacgcgccg cgatatatga
    12421 agcgcgcaca aaacaggcaa aagcgggatt taaaaatcct gtaaccgatt aattaatcaa
    12481 cataaggaaa agcgacatga aaatttgtat cgacgacggc tccaccaaca tcaagctggc
    12541 atggactgag aacggcgaac gccgcaacgc catcagcccg aacagcttca agtcggaatg
    12601 gtctgcgcca ttcggtggca cgcagcccgc gaactacatg cttgatggcg tgcgctatgg
    12661 ttttgatccg gtcagcgatc gctttgtcca gacgaccgac acgcaatacc agtacagcga
    12721 tgtgaatgta attgccattc atcacgcgct ggtcaaatca ggcatcacac cacaggaagt
    12781 ggatgtggtt gtcaccctgc cactgagcga gtatttcgat acaaacgcac agccggacat
    12841 ggccaacatc aaccgcaaaa aagcgaacgt catgcgcccg gtggagtacc agaacggcga
    12901 agcattcact atccgtaacg tacgggttat gcctgaatcc attcctgctg gctttaaggc
    12961 accggctgac atgagtccgt ttgaatccct gctgattgtg gatttgggcg gaaccacgct
    13021 ggatgtggca aaggttcagg ggcaactggc aggtatcagc caggtgtttt gcgatccaca
    13081 cgtaggcgtt tctctgatgg ccgatgccgt actgtcggtg atggccacta acggtatgcg
    13141 caccagtcac cacatcgcca ataccattat cgaacatcgc catgatgaag cctggctgcg
    13201 ccagcacatc cacaatgacg cgcattacgc cagcctgatg gcggttattc gtgaaaagga
    13261 agaaacactg aaacaacgcg tgatccgcgc gctggcggtt ttttcgggtt acgggcgggt
    13321 gatggttgtc ggtggcgggg cggagattgt ggcaaccgct atccgcgaag cctgcggagt
    13381 taatgcgact ttcatcgcgg acggggtgcc acagtttgct ctggttaatg ggttgtacgc
    13441 aatgaacaag gagtaaacca atgacgacac caaccagacg gataagtttc tatctgaagc
    13501 ccgccgccgt caagagtgaa caggaggcgt gcaattacct cgatagcctg ccagcctccg
    13561 aacgcagccg cgcgcaacgc gcggcctttc tggctgggct agcactcata aagcgcaacc
    13621 cagcatttgc ctactggatg gccgaatggc cagaggacaa cctccccttc aataccgtta
    13681 gcatcaagga aacataccag gctaatcagc
//
LOCUS       DAZFKY010000127        13622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFKY010000127 DAZFKY010000000
VERSION     DAZFKY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..452
                     /locus_tag="ACHKCL_003326"
     CDS             <1..452
                     /locus_tag="ACHKCL_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5764225.1"
                     /translation="HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ
                     KKESEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVVNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            718..2955
                     /gene="nrfB"
                     /locus_tag="ACHKCL_003327"
     CDS             718..2955
                     /gene="nrfB"
                     /locus_tag="ACHKCL_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5764226.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2942..5914
                     /gene="nfrA"
                     /locus_tag="ACHKCL_003328"
     CDS             2942..5914
                     /gene="nfrA"
                     /locus_tag="ACHKCL_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5764227.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5915..6805
                     /locus_tag="ACHKCL_003329"
     CDS             5915..6805
                     /locus_tag="ACHKCL_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5764228.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6988..7749
                     /gene="envY"
                     /locus_tag="ACHKCL_003330"
     CDS             6988..7749
                     /gene="envY"
                     /locus_tag="ACHKCL_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO5764229.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7795..7871)
                     /locus_tag="ACHKCL_003331"
     tRNA            complement(7795..7871)
                     /locus_tag="ACHKCL_003331"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7835..7837),aa:Arg,seq:tct)
     gene            8114..8746
                     /gene="fimZ"
                     /locus_tag="ACHKCL_003332"
     CDS             8114..8746
                     /gene="fimZ"
                     /locus_tag="ACHKCL_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO5764230.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8749..9264)
                     /gene="sfmF"
                     /locus_tag="ACHKCL_003333"
     CDS             complement(8749..9264)
                     /gene="sfmF"
                     /locus_tag="ACHKCL_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO5764231.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(9275..10282)
                     /gene="fimH"
                     /locus_tag="ACHKCL_003334"
     CDS             complement(9275..10282)
                     /gene="fimH"
                     /locus_tag="ACHKCL_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5764232.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(10295..12904)
                     /locus_tag="ACHKCL_003335"
     CDS             complement(10295..12904)
                     /locus_tag="ACHKCL_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5764233.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12935..>13622)
                     /gene="fimC"
                     /locus_tag="ACHKCL_003336"
     CDS             complement(12935..>13622)
                     /gene="fimC"
                     /locus_tag="ACHKCL_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5764234.1"
                     /translation="TKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTAVW
                     IRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRE
                     SLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSG
                     NYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGS
                     VTPVREVNLN"
BASE COUNT         3570 a         3417 c         3280 g         3355 t
ORIGIN      
        1 gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat gaacttattg
       61 atttcacgtt tgaatggaaa gggctgaaga aattatgcgt ggcagtctcc tttcggtcca
      121 taatagcaga acaaaagaaa gagtcagaaa tgacggtcag atattatatc agttctgctg
      181 atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg gagaataagc
      241 tgcactggcg tctggacgtg gtagtgaatg aagacgactg caaaataaga agaggaaatg
      301 cagcagaatt attttcaggg atacggcaca ttgctattaa tattttgacg aatgataagg
      361 tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctgg
      421 cgtcagtctt tgcggggagc gggctttcgt aatcttgccc tgcctgctca cctccaggca
      481 cttccactac atcatgacct gatcggtgcc accactcctc ttcactatga cgttaacctc
      541 ccctccttga caccacgctt ttgtctacat tctacgcaaa cggcaacttt tataaccagt
      601 gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt cacaattcat
      661 aaaaaacctt aatatacgcc accctaaaca taaccagcgt taatgtaagg tttttgtgtg
      721 gactggcttc ttgatgtttt tgctacctgg ctctatggct taaaagtaat cgcgataacg
      781 ttagcggtca tcatgttcat cagcgggctg gacgattttt ttattgatgt cgtctactgg
      841 gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat gagttaccgc
      901 gaactgtata aaccagatga aaaaccgtta gcgattatgg ttccggcgtg gaatgaaacg
      961 ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc tcgactacga aaactatcat
     1021 atctttgttg gcacctaccc caacgacccg gacactcagc gtgatgttga cgaagtgtgc
     1081 gctcgcttcc cgaatgtgca taaggtagtc tgcgcgcgtc ctggccccac cagcaaagcc
     1141 gactgtctga acaacgtgct ggacgccatc acccagtttg agcgtagcgc caatttcgct
     1201 tttgctggtt ttattctgca tgacgccgaa gatgtgattt caccgatgga attgcgtctg
     1261 ttcaactatc tggtcgagcg taaagatctg attcagatcc cggtgtatcc gtttgaacgc
     1321 gaatggacgc acttcaccag catgacttac attgatgagt tttcagagct gcatggcaaa
     1381 gatgttccgg tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt cggcacctgt
     1441 ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg acggtattgc tttcgacgtg
     1501 cagagtctga ctgaagatta cgacattggc ttccgcctga aagaaaaagg tatgacggaa
     1561 atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg taaattttta
     1621 cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat atttccccga taccttttcg
     1681 actgcggttc gacaaaaatc tcgctggatc atcggcattg ttttccaggg ctttaaaacc
     1741 cataaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg caaaggggca
     1801 atcagtaact ttgtcagctt cctcgcaatg ctggtgatga tccagctttt gctgttgctg
     1861 gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt ctatttttag cggcagcgca
     1921 tggttaatga ccctgctgtg gctaaacttt ggcttgatgg ttaaccgcat cgtgcagcgg
     1981 gtgattttcg tcactggcta ctacggcctg acgcaggggc tactatctgt cctgcgtctt
     2041 ttctggggca acctgattaa ctttatggcc aactggcgcg cgttaaaaca ggtacttcaa
     2101 cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc atgacttccc cagcgtcact
     2161 ggcgataccc gctcgttgcg cccgttaggt caaattctgc tggaaaatca ggtcatcact
     2221 gaagaacagc tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct gggcggttca
     2281 atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc agagcaaaac
     2341 ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt cctcgctgat tgccgaaatg
     2401 ccggcctccg tggcgctgca ttatgcggta ctgccgctgc gtctggaaaa tgatgagtta
     2461 attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac gcgtaaagtc
     2521 ggacgcaaag tgcgttatgt cattgttctg cggggacaaa ttgtcacggg gttacgtcac
     2581 tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc ggttcagcat
     2641 cagtggctca cggaacagca ggccggtgaa atctggcggc aatatgtgcc gcatcagttc
     2701 ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat taacgtgttg
     2761 ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac cgaaggcgtt
     2821 atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca agtttcgatg
     2881 caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact ggagtccgaa
     2941 aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc tgacgtcttt
     3001 attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc tggggctgag
     3061 cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg cacagaaaaa
     3121 taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg tgccggataa
     3181 tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg atgaccgggc
     3241 acggctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac ttgagcgcag
     3301 tctggcggct attccggttg aagtgaaaag cattacaact gttgaagaac tgcttgccca
     3361 gcaaaaagcg tgcgatgctg cgccgaccct gcgttgtcgc agtgaagtcg ggcagaatgc
     3421 cctgcggctg gcacagttac ctgtcgccag agcgcaactg aacgacgcga cgtttgctgc
     3481 atcgccggaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct acctgaaaca
     3541 atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat taagcgcggc
     3601 agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctggacgatc ggatcctggc
     3661 actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg cgaccgcgct
     3721 ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc attgaaaata agccgctgtt
     3781 taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca gcgccaaccc
     3841 cgttcaggca ttggcgaatt atacggtgca gtttgccgat aaccgccagt atgttgttgg
     3901 cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa aactgctcgc
     3961 caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg cgacccgtaa
     4021 caaggctgaa gctctgcgtc tggcacgctt gctgtatcag caagaatcgg caaatcttac
     4081 ccgcctggat caactaacct ggcaactgat gcagaacgag cagtcacggg aagctgccga
     4141 tttattgctg caacgctatc ctttccaggg cgatgcgcgt atcagccaga ctttaatggc
     4201 gcgactggcg tctctgctgg aaagtcatcc ttacctggca acgccggcga aggtggcgat
     4261 tttatcgaaa cccttaccgc tggcggagca acgtcagtgg caaagtcagt tgccgggtat
     4321 tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt cctacgatgc
     4381 cgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg tggcgttgta
     4441 tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg cggtagccta
     4501 tcaggcgtat caggttgagg actacgccac cgcactggcg gccaggcaga aaatcagtct
     4561 tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg cggcaggaaa
     4621 tggtgcggct cgcgatcgct ggctacaaca ggcagaacaa cgtggactgg gaagcaatgc
     4681 cctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac tcgcactgaa
     4741 cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg cgcgggcgac
     4801 aatttatcgc caacgtcata atgtcccggc cgcggtgagt gatttgcgcg ccgcgctgga
     4861 actggaaccg aataatagca acacccaggc agcgcttggt tacgccttgt gggatagcgg
     4921 tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggctac cggacgatcc
     4981 ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc ctgcgacgca
     5041 gcactacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa ccccactgac
     5101 cccagagcaa aatcagcaac gcttcaattt ccgccgtctg catgaggagg tcggtcgccg
     5161 ctggacgttc agtttcgatt cttccatcgg cttgcgttcc ggggcaatga gtaccgctaa
     5221 caataatgtt ggcggcgcag cgccagggaa aagctatcgt agctacgggc aactggaagc
     5281 cgaataccgc atcggacgca atatgctgct ggaaggcgac ctgctctcag tttatagccg
     5341 cttctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc cgatgtccgg
     5401 caccggtctg cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg tcgaacagca
     5461 gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca gcgcctcatt
     5521 ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct ggtttgccca
     5581 aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt ggacggcaga
     5641 ttatcgcgtc agctggcatc agaaggtagc taacggacag actattgagc cttacgctca
     5701 cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg gcggtgtcgg
     5761 ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc acaaagtcag
     5821 tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg gagagcgcaa
     5881 caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt tcgtattgct
     5941 gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct ggcaaccaca
     6001 aaaccgtgat agtcaggtta ccgataccca gtggcagggg ctgatgagtc agttacgttt
     6061 gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat tcacccagcc
     6121 agaacagcgc gcgttattgt ttaagcgggc cgcagctgcg caacaggctg gcctgaagct
     6181 gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt catccgcggc
     6241 gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca gattatggag
     6301 tgccgtacct ggcgtaacgc cggatggctg gtacttcagc gctgaaattg acgacctgaa
     6361 ctggcgcagc gaagccgccc gtcagccttt gctaacatgg ttaaacaacg cgcagcggct
     6421 gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg gaaacatgtc
     6481 gccagatggc tatcgccaac tgctggaaca cgttaaagca accggcgtta atgtctgggt
     6541 acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt atctacaggc
     6601 cagcgccgat tgccaaagct ccgccccagc aagcggcatt gtttatgaac tgtttgtcgc
     6661 cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac gcagaaattg cctcgctgtt
     6721 agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct atttgcccgt
     6781 cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg acctggctta
     6841 cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt gctaaagaaa
     6901 gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat cctttgagcg
     6961 gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg
     7021 atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt tacccttccg
     7081 aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc aacgttaaat
     7141 cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt gtttttaaat
     7201 aagaacttaa cctgtgtaaa gccctggtcg cggctggcta ccccggttat cgcttgtcat
     7261 agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca acccagcaaa
     7321 ccctgcgaag cggagttgac gcgcgcattg ctttttaccg tattgtctaa ctttcttgag
     7381 caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg cgacagcgtc
     7441 tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt cgccagttcg
     7501 ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac cagctatagc
     7561 cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat ggataacaaa
     7621 aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt tatctctgtt
     7681 tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg acaaaaagtg
     7741 atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaataaagaa gaaatggcgc
     7801 gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga
     7861 gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt
     7921 gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct
     7981 gatttatttc gctatagcgg caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac
     8041 ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat
     8101 gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag
     8161 aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga
     8221 tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga
     8281 tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag
     8341 cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat
     8401 acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc
     8461 cgttcagatg atcctctccg gatacacgtt ttttcccagc gaaacgctta actatataaa
     8521 aagcaataaa tgtagtacga atagttcaac ggtcactgtg ctatctaatc gtgaagtgac
     8581 catattacgt tatctagtta gcggattatc taataaagaa attgccgata agttattact
     8641 tagcaataaa acagttagtg cgcataaatc taatatttat ggcaagctag gtttgcattc
     8701 aattgtagag cttatcgact acgccaaatt atacgaatta atataatatt aattataatt
     8761 gatcataaat atcgcatccg ctttcgccac acctggccga acaccgctgg ctaacgctcg
     8821 ataattggca aaaaagttta gtgtgacatt gccatttgca tctacttcct cagtcgggct
     8881 cgcctccccc agtgcgagcc gggagcgatc gctattacgt aattcgatgg cgacggtttg
     8941 tgccattgcg ggatcatcca gagccagcag gttggcatca gatgccggcg ttcccgtaaa
     9001 taaaatcgca actgaacccg gaggacatcc ctccagccgc aggctaaaag ggaggagtgc
     9061 cgtggtatcg ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat ctaccgtctt
     9121 atcaatatcc gctgtgttta cggtacagga gaaatcaaca acgttaccgt gcaaattgat
     9181 attaatcgtt cctaaagggt cagctgccca tccactggaa ctccacagta acccgcagaa
     9241 acagctaaag aatactcttc tcattctccg tacctcattg ataatcgacg cgtaaatacc
     9301 ccagcgcgct aaacggccct tcggtcggtt tttgaccggt aatactgata ggccaggcgc
     9361 gaagtgtgac attggctgcc gcagctgcat ccagacggaa aggaataacg ctattgagat
     9421 cgttaggcgt gatcggcgta tcgttctgat cggcgacaat aaaacctaaa tcctgattgt
     9481 ccgacaccat cgcctgacca gaaacggcac tggcttccag acgcattgtt aaataagcct
     9541 gcgcagcaac attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg acaccagcag
     9601 gccgattacc cggccctgcc gcactaaata acgatgcgcc gatatcacca aaatcaaatt
     9661 caacaatctg cccggcattt aattcgcagt tttgcggtac ttcaacccgg ccaccaaaac
     9721 taatggtata aacaggtgta accaacggat cgtaaggcgt tgatgtcaca tacacggtaa
     9781 acatcacctg tctggggatc tccaccatgt taataaaggg ccgaataacc tttaaacgaa
     9841 atatcaaacg agaatccatc accccgaacg gaaggttttg cgaaacgtta ggatctgttc
     9901 ccatacgaat ataattttgc gggggaaatt gaatatccat cacactatca accagactca
     9961 tcgcacctaa tagataatca tttaattgca tatatttata attatcgata gtttcctgaa
    10021 caataaagtt agtgacataa ctacggtatg tataattcac cagcgtgccg ggtggacaaa
    10081 ttgctgagac acctacccag ccggattttt ccggtaaaac aacaatctgc cctggctggt
    10141 tattggtgct attaaagaca tttgagagat catagtgttc atcttctgct acgccagtac
    10201 tatttgcaca aacagtagcc caggatatgt ttgccagaca caaacatgcc atcgccaata
    10261 ataatctgca gattatcttc attgataatt tcctttatga gccaggatgt gtgcaaactg
    10321 ccgaaataac agtgacggct tgctgtaagc tttgcttcgg caatacatag tgcgcaatgc
    10381 agcgtgagtt cgcgccgtct ccccactgaa ccagtaattc accagacaat ggcgcaccac
    10441 ttaaataaac ttgcccgtca tcacccacca tactggttat tccggtactg ttttcccgta
    10501 ccagtgatcc aaacggcacc ggttttccgc cctgggtaac ggtaatgagc gcccgcacgc
    10561 ctatgcgggt atcaaaattg gcacgaacca acgcgccttg cgtcggcaca acgctgctaa
    10621 tatttttttc aacatcgatg gaattcccca tcgtattggt atcaagcgcg atacggttat
    10681 accgataaac cgtggcatac ggcatcaccg catagccgcg ccagtcggtt aaaatgccag
    10741 tttgattttc aatgcgtaca ccgcctgcgc caggcgcttt aatcaaaaca ttggtatccc
    10801 ctaaaggctg gctcagcgtt atgccatttt catgtccgac cacaccgcca gacagctgcc
    10861 agttaacgtc atgttgatcg cggtcgtagt tatacccgac ccccagcgta ccgtaagcgg
    10921 cctgccagtt tgccgtggcg ctgcccgtat acccattatt cgaggtatca ccctggctta
    10981 cgtgataact caggttgtgg ccttccagta aggtcccacc tacacctgcc agccagctat
    11041 tttgcccgtt gctgttacgg ttggcgttaa aggaggcata agcgcggtcg agcgcatttt
    11101 cccgggtgta gcgacgtttg gtcagaacat tgaaaggcac tgaaacatta agtccgacaa
    11161 tacgttcgtt atcggggatc cctacagatt cattccacga aaatgagagt gaataactga
    11221 tgccaaccca gctgctggta taccccacct gataccaggt atctgaatcc gaagtattcc
    11281 agtatttttg atgggtacca gagatataaa gcgagccaaa gtcattaagt gattgtgaaa
    11341 tatttaactg caaacggtct ttacggctaa agcgtaaatt gtggtaattc acgattatcg
    11401 gttcatcccg atgctcaccg tcataatcgt aatcatattc gtacccctcc attcgacgat
    11461 acgcaacatc atcaagggta taaaaacctt gtgtcgaata gcggtaaccc attaactgaa
    11521 aattggtgcc gaaggtgttc atcgatttcg catagaggaa gcgaatggaa tccccctcat
    11581 gacgactgtc gtcggctaac tgactgcgcg catgggtcac atccagcgac actgcgcccc
    11641 agttcccgag gttgcgcccc agccctaata aaaaggcggt gtaattggca gataattgcg
    11701 tcccgccgta ggcagtaaat tcctgtggta aaccgccgag tgccgtgccc tgaaaaaaga
    11761 aaggcgatga ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg tcaaatttga
    11821 aacgcccttc gcgttgtaaa attggcactg ttgaatacgg aatagtgtaa ttctgctgat
    11881 tgccatcgcg ttcgtcgatg gtgacgtcca gatcgccatt tgaagatgtc gggtgcaaat
    11941 ctgtaatttc aaaagcgccg ggggaaacat agctttgata gataataaaa ccattttgcc
    12001 gaatcgttag ctgggccgcc gtacgggcaa tcccacgtac cgttggggca aacccttgct
    12061 ggctatcagg atacatatta tcggaagaat aaagccgtac accacgaaat ccaacaccat
    12121 cgaaaatatc acttcctgta ttgccgtctc ccattaccag ttcacttttc agcggaataa
    12181 tggcgcgctg tacccaggtg ccaatattat tccactgttc tgaatgatat ccatttccgc
    12241 gaaaatagtt ccaggaacca ttgttgcgta aacgccacgg gccaatatta atcccgctga
    12301 gctcactaaa aaaatagcta tcgttaccat tacctctgtt accggtgaaa ttataattca
    12361 ggagtaaagc aggaatacct tcatcccact cttcaggcgg aatgtaaccg tgagcgctac
    12421 tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa tcaaagttaa
    12481 ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc tgctgggcta
    12541 actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg ttaaaacagg
    12601 gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat acgatatcct
    12661 gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc gcaggttgat
    12721 gattacctct ctcaaagcgc gataaatcag caacgaatgt gccattctct aataaaaatg
    12781 ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca agagatgaat
    12841 aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta gtgggtattt
    12901 tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact tctctgaccg
    12961 gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc actcctgagg
    13021 gtaagggaat ttgtgaatta actctgggtg cagccatagc attccccaac ttttggctgc
    13081 ccacttgtaa gttaaccagg gtgacataaa aaggtgatgg attatgaaca ttgatatagt
    13141 taccggatcg cgaaaacttg agtgtatccg gcgcttctgc gggtaattct tgtagttgaa
    13201 ttggacggag aaataacttc atgcgcgata aaatcgccag ttgcaaaaca ttcctgccgt
    13261 tcaatgcatt tttatctacc gaaggaatcg tcttaacatt catccagaac agagactcac
    13321 gatctgctgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg cttttgggtt
    13381 cactaacaaa tagtggcggt gtaatgataa atgacttttc ttttacaccg ctgctgtttt
    13441 caatccacga attgaccaga aaacgctcat tggtatggct atttctaatc cataccgcag
    13501 tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc cctccggcag
    13561 catggacgct ggccgaaatg attaaataaa atataatgag catcaataac tttattttag
    13621 tc
//
LOCUS       DAZFKY010000128        13459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFKY010000128 DAZFKY010000000
VERSION     DAZFKY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            30..860
                     /locus_tag="ACHKCL_003337"
     CDS             30..860
                     /locus_tag="ACHKCL_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5764235.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1001..1774)
                     /gene="uxuR"
                     /locus_tag="ACHKCL_003338"
     CDS             complement(1001..1774)
                     /gene="uxuR"
                     /locus_tag="ACHKCL_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5764236.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1989..3449)
                     /locus_tag="ACHKCL_003339"
     CDS             complement(1989..3449)
                     /locus_tag="ACHKCL_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5764237.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3530..4714)
                     /gene="uxuA"
                     /locus_tag="ACHKCL_003340"
     CDS             complement(3530..4714)
                     /gene="uxuA"
                     /locus_tag="ACHKCL_003340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5764238.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5054..6397
                     /gene="gntP"
                     /locus_tag="ACHKCL_003341"
     CDS             5054..6397
                     /gene="gntP"
                     /locus_tag="ACHKCL_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO5764239.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6571..7473)
                     /gene="fimH"
                     /locus_tag="ACHKCL_003342"
     CDS             complement(6571..7473)
                     /gene="fimH"
                     /locus_tag="ACHKCL_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5764240.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTKNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7493..7996)
                     /gene="fimG"
                     /locus_tag="ACHKCL_003343"
     CDS             complement(7493..7996)
                     /gene="fimG"
                     /locus_tag="ACHKCL_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5764241.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8009..8539)
                     /gene="fimF"
                     /locus_tag="ACHKCL_003344"
     CDS             complement(8009..8539)
                     /gene="fimF"
                     /locus_tag="ACHKCL_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5764242.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8549..11185)
                     /gene="fimD"
                     /locus_tag="ACHKCL_003345"
     CDS             complement(8549..11185)
                     /gene="fimD"
                     /locus_tag="ACHKCL_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5764243.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11252..11977)
                     /gene="fimC"
                     /locus_tag="ACHKCL_003346"
     CDS             complement(11252..11977)
                     /gene="fimC"
                     /locus_tag="ACHKCL_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5764244.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12014..12553)
                     /locus_tag="ACHKCL_003347"
     CDS             complement(12014..12553)
                     /locus_tag="ACHKCL_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764245.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     HRVTGGIANAQAWFSLTYQ"
     gene            complement(12618..13166)
                     /gene="fimA"
                     /locus_tag="ACHKCL_003348"
     CDS             complement(12618..13166)
                     /gene="fimA"
                     /locus_tag="ACHKCL_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5764246.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3267 a         3464 c         3258 g         3470 t
ORIGIN      
        1 cagtgaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat gtccttcaga
       61 gtcaaattat tacagataat cacttccttc atcatccaaa agtcgaaagt gagttaacac
      121 gtaaatatga acgtgcacgc ctggatacgg aaaatatcta tcttctgcct cttgctcgtg
      181 gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt gatattagta
      241 aagaatcctg gccatttaat tatgtgacag aagcatgcag agaatccgat ggtattacaa
      301 caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac gaaatatatg
      361 gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta cggctaaatt
      421 tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa ataacattag
      481 gccctggttg caatgttcca ggttacgccg gaaccacgat aggatatatt tcaacccttc
      541 cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc tatattgatc
      601 agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc gcattactta
      661 atgctaatat tgagttgggt aatgacccaa taattggtat agaagcctat ccaggtaccg
      721 cggagatcca tgcaaaaatg ggatataaag ttatccccgg tgatgaagat gcgccactca
      781 aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat
      841 ggaattatat tggcaaataa ccactgagaa gaatgagtac taacattcag gttatataga
      901 tatttaaaca ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga
      961 aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca
     1021 gcggccatga atcaaacaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca
     1081 gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat
     1141 cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca
     1201 ggctgtcatc cagatggctg tgcaactgga tccacattgg gttgttttcc cgccactgcc
     1261 aggactgacg gaacagctcc accagcatgc tgttatgcgt tgcttcggca atagcgagat
     1321 ggaactgcat gtcaccgctt tcgctgctgc ccggcgcact ggaagccagt tcacgctctt
     1381 ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa
     1441 actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa ggacccgcat
     1501 cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagaacat
     1561 agataccggc accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt
     1621 cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggtagcc
     1681 gttcaccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct
     1741 ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta
     1801 atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac
     1861 ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca
     1921 aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa
     1981 tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat
     2041 gccgcagtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg
     2101 ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg
     2161 aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg
     2221 gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc
     2281 aggtgcagac gcaccgggtc cagcagacgc tgcggtaact tctggctgcc gtccatcgca
     2341 atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca
     2401 taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc
     2461 agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat agtttcatag
     2521 ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat acgcagtttc
     2581 atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccgga
     2641 cgaccattaa cgaagttatc ttcaataacc cactgacgga acggttcgca ggcaatggcg
     2701 cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc
     2761 ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgct
     2821 agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt
     2881 tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc acgacgcaga
     2941 cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg
     3001 tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct
     3061 ttttccgtga ccgtcaggga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata
     3121 ccttcgcagc cgtcgatttc cgggtgcagc gcttctttca tcgaaccgat aattttcagc
     3181 tcggtgcttt ccgcgccttt ttccgccacg gtgtacagca gttgctgttt tttcaggttt
     3241 tcgatcagca cgcggtcgtt gcctggcatc aggttaactt cgcagatacc ccagtcgctg
     3301 tcggtgcttt ccagcagatg atgggtgtac agcgcctggt gcgcacggtg aaacgccccg
     3361 caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc
     3421 ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg
     3481 acggcaagga agaaggagcc ggcacggcgc gcagccatgc cggtgggtat taatggctaa
     3541 agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac
     3601 caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt
     3661 ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt
     3721 caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt
     3781 ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg
     3841 gaccgaactg cttgatcata tcaaccaaat cgttgtcagc acgcacgccg taggaaccgg
     3901 tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt
     3961 caatggtgga aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa
     4021 cagccatacg gacgccaact tcttcggcaa ccggaataat cgctttcagg aagacagcaa
     4081 agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc ttacggaact
     4141 ggtcgagggt gtacccttct tccgcacctg gcagaccagc gatgatgtta cgggtcagac
     4201 gcgctttgtc ttcatcgctc atagtggcga agcgttcagc tgcctgagca atttcttctt
     4261 cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt
     4321 cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac
     4381 gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca
     4441 ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt
     4501 tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa
     4561 cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt
     4621 gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat
     4681 cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt
     4741 tccacagcaa agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac
     4801 cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat
     4861 gtcaacccga attctgaaat tggttaacca catcacaaga atttcactct catggttgtt
     4921 gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg
     4981 caacaatggt tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt
     5041 aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggtctgat
     5101 gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct
     5161 gtccgtcggg atgctggcgg gcatggattt gatgtcgctg ctgcacacca tgaaagcggg
     5221 cttcggcaac acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa
     5281 attgatggtc gactccggcg cggctcacca gatagcgcat acgctgctgg cgcgtctcgg
     5341 tctgcgctat gtacagctgt cggtgattat catcggcctg atttttggtc tggcgatgtt
     5401 ctatgaagtg gcctttatca tgttagcgcc gctggttatt gtcattgccg ccgaagctaa
     5461 aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact
     5521 gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcag atatggggat
     5581 ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct
     5641 gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc
     5701 ggtagatatg aacaatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc
     5761 gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata cccctgcctg
     5821 ggaagtggtt aactttatcg gttcctcgcc gattgcgatg tttattgcga tggtggttgc
     5881 attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga acgcttttga
     5941 aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa
     6001 gcaaactatc atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa
     6061 tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg cgacgggtca
     6121 gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc ttgatccagc
     6181 aactggtcag ctggttggcg tgaatccggc gctgctggtg ctggcgacgg ctgcgggttc
     6241 caacaccctc acccacatta acgatgcatc tttctggttg ttcaaaggtt actttgacct
     6301 gtcggtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg
     6361 gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag cctgccaatg
     6421 gcaggctcag ttcggattgt cggcaaagtg cgctttgttt atgccagatg cgacgctgac
     6481 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg
     6541 tacctgcatt agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat
     6601 cgattgcaca ttccctgcag tcacctgccc gccggtacgt gcgtaatttg ccgttaatcc
     6661 cagacttacc gccgaagttc ctactgctcc taacgatacc gtgttattcg ctggaataat
     6721 cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg acgcggtatt
     6781 ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc ccaggttttg
     6841 gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt ccggcagagt
     6901 aacggtgaca tcacgagcag aaacatcgca gccgccagta ggcactacca catcattatt
     6961 ggcgtaaata ttccacacaa actggaaatc atcgctgtta tagtttttgg tctgtcgcaa
     7021 aataagcacg gcaattaatg agccagcttt aatcgccacc ccgcccgcac tgctcacagg
     7081 cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa caacccgcgg
     7141 cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg tcccggaaaa
     7201 attagataac acgccgccat aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt
     7261 ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca ggttttgccc
     7321 cacattcacg gcaggcgcaa ggtttacata aacattagcg ctgccaccgc caatagggat
     7381 agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc agcccatcag
     7441 cagtacagca aacagggtaa taactcgttt cattacaatt atctcttcgg gttcagctgt
     7501 aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg ccatttaccg
     7561 tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc acctgaactg
     7621 ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct aactggatgt
     7681 tttgcgcggt cccctggttt ttataatatc cggtactgtc ggctgccccg ctgaagctgg
     7741 cagtgaccct tgacgttccc accggacaat tagtcaactc aagcgcaaca tcatgccagg
     7801 ccgatgccgc cccggcagac atcagactga aagaataaag atcgccgaga tcaaccgtgg
     7861 cattggtggt ggaaactgtg cacggtttgg cgacgacctt accgttcacc gtgatggtga
     7921 catcggctgc ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat aaatacccac
     7981 gtttgcgcca tttcatgagc atctccagtt actgatattc aagagtgaag gtagccgtgg
     8041 cattgatatg ccccgcagtg acaggcacct gtgtcgccat tagccgggcg taaaaattca
     8101 acgtatttgg tttacccggc gtcagggtcg tccacgaaat cgcggacgat ggagcattaa
     8161 ggggtatctg attttgctgc tcattcagaa gctgtattcc cagtcccgaa gccgctgaca
     8221 ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacgccg gtaaacccaa
     8281 cttttacggc agaaacggcg ttaccacagg gtgacagcaa aatacgaaat ggaacgacag
     8341 gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa
     8401 aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg cggatagtaa
     8461 tcgtgctatc cgcagccaaa gcgtgactca ctgccagcca caaaaaagcg cacagaagat
     8521 aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc tgggttaata
     8581 actgctgctg actctctggt ggcagttgat aattggcgac acaatgagca ttttcctctt
     8641 ctccccattt cacctgaact tttcccgcta gaggcattcc gctgaggtaa acctgaccat
     8701 tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc ccaaacggca
     8761 gcggcttatt attgtgggtt agcgtcatga gcagttttat cccaacgcgc gctttaaact
     8821 ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta tctaaatcga
     8881 cgttatcagc cagggtattg gtatccagcg ccactctatt ttcccgatat tcagtggcat
     8941 aaggcagcac ggcataaccg cgccagtcgg tacgcacccc cgtctggttt tcgacttttg
     9001 catcttttgc gccaggcgct ttaacaagca ccaccgtatc gtttaacggc tgccccagcg
     9061 ttacgccatt ggcatgagcc agtaccccac cgctgactcc gtaatagagc tgcttaatat
     9121 catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa ttcagcgtgg
     9181 cgtagcctgt gcttccgcta ttaccatcgc ctcccccggc atagccggtt tgcacgctat
     9241 agctgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg gtcatccgac
     9301 cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga gatttactgt
     9361 cagaacgcag ccagtggctg aaaggaatat tgacgttacg cgctaacatc tgatcacgtc
     9421 ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata tcttcgaacg
     9481 cagtatttaa tccagcctgg aattgctcat cgacattact cgttccccaa taagtttgat
     9541 ggctaccact caaatacagt gttgatgagc gcccgagttg ctgagtaacg gtgagttgta
     9601 atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa
     9661 taactccgtc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt gtatcagcga
     9721 aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc gtgcctgact
     9781 cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg
     9841 gaagtgtgga attagcctga gtcatatcca cagacagagc gcccagtgcc cccatatttt
     9901 tcccgatacc aaaattaaaa gcacgataac gatctgccag ttgcgttcca ccatatattg
     9961 tccagccagc tggaaggccg tggagtaatg tactttggaa aaagcggggt ttttcctgtt
    10021 gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc ccttcacgtt
    10081 gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg tcagcctctt
    10141 taatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg atggtaaaag
    10201 gccccggcgg caccgtacta ttataaatgt catacccatt ttgtttaata gtgacctgtg
    10261 cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta tcgggtaaca
    10321 tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa atatcaccct
    10381 gagtataacc atcacccagc gtcagccggg aacgtaacgg tattatgtct cgctcaagcc
    10441 aggtattgat atgctgccat ttatttttgc tacctgatga tctgtcgcta ctgttataac
    10501 tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc tgtaggttta
    10561 aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg ctgaaattat
    10621 aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata taaccacgcg
    10681 cgcgattact cataaatgcc tgagggatcg tcaggttcag tcgctgctga ccaacatcta
    10741 aatgcgcagt agcgtcctgg accattgtgg ttaatggcac acaggcatca tccgccagca
    10801 gattcatacc ggcgacagaa gccgtattca gccccatact ggcgagttgc gcgcgtgtca
    10861 ggcagggaac aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca
    10921 tataaccatt attcaaatag atatcgacgc gatacgtccc tggcggtaat tcttgcccat
    10981 tttcaaaacg cgataaatcg gccacagcct ggggatcatc cgctaaaaag cgcggattaa
    11041 aatagagttc ggcagatgac aaaggtgcct gtacggcaaa agcacaggcg acaaagagcc
    11101 ggacaaaaaa accagccaaa cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt
    11161 aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg gcagtcaatt
    11221 ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc attttggggg
    11281 taagtgcacc ataatcattt attgttcggt aagtaatatt gcttcccgca tcagaaggca
    11341 atttaaccgt gctttcgccc attggaggca ccaaggcatt ttcaagaacc cgggctccgg
    11401 cattcaactc tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc agagaattcg
    11461 cgctacgacg aaatcttaat ttttctgcgg cctgatcggg tggcaacgct aatttagccg
    11521 gacgatagta cagtttaatg cggctgataa ttgcgagctg tagcgtattc tcagtcaatt
    11581 ttgatttatc cattgacgga atcgctttaa cgttcatcca gaataaactt tcccggtcct
    11641 gtggcaattg gttatttgtt gcatcaagaa tacgtaaggt attctctttt tttcccttca
    11701 tcgcaaacag aggaggcgtc acgataaaac gaccatcctt tacaccatcg gcattttcca
    11761 cccatgattg aattaaatag gtactatttt catcattatt tgtcacggca agttgcactt
    11821 gtttttgccc tgccggataa attatgcgag tcgcacctaa ggccactccc gcttcagcgc
    11881 gtccggcaac catcattgcc atgaacatca ggatacctgc cagcaagcag aatgttattt
    11941 cctgcgattt ccttacattg acgtttttat tactcactgt cctgttcctg ttatcacatt
    12001 atctgctgaa caattactga taggttaaag agaaccaggc ctgtgcattg gcgatgccgc
    12061 cagtaacccg atgcccggta gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg
    12121 agtaaagccg tttccagttt gctggaggac gattaatcgg tacgaggttt ccttcatcat
    12181 caaacaacgc tacgccaata ttggtggcta tccctggccc ctctcccacg gaaagcacat
    12241 ccggattttt accatccgcg acaccgtgaa acgccacacc cacacgttca ctcaccaccg
    12301 tgctacattc ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc cctaccgcat
    12361 gaaaccggtt actgctgatt tgccccatat tgaccgtcat ttgtttatca ccggcttcaa
    12421 tcaggcaagt ttccgcaata atgacgccct gaaattgcat atttccgccg ggcggcgtgg
    12481 tattccattt atttccggcc agagcaaaca ttggcagcaa cgacgctaat agaaataatc
    12541 tcttcctttt catcgttatt tttatcgcac aagggtgggc atccctgccc gtaatgacgt
    12601 ccctgaacct gggtaggtta ttgatactga accttgaagg tcgcatccgc attagcagca
    12661 cccggggttg cctcgccgat tgcataataa cgcgcctgga acggaatggt gttagtaccg
    12721 ttattcaggg ttgtttgctc actgaatgtc gcaccatcca gcgtcagcgc agcacccgtt
    12781 ctgtccagga tctgcacacc aacgtttgtt gcgctacccg cagctgaact ctgcagagcc
    12841 agaacgttgg tatgacccgc atcaatcgcc gtacctaaaa aggcaacagc ggctttagat
    12901 gcaacattgg tatcgcaatc attcagctga atgttaaaac cgacagcaga actggttgct
    12961 ccgtcctgtg ccagcgatgc ggtacgaacc tgtcctaact gaacggtttg atcaacagag
    13021 cctgcatcaa ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac ggtcccacca
    13081 ttaaccgtcg tggcagcggc cagagccgct gtagaactga gggacagagc cgacagaaca
    13141 acgattgcca gagttttaat tttcatgctg ctttcctttc aaaaaactat ttctaaatcg
    13201 acatgggcag tcgttctgta cactttgttt tgtcaacgag tttgactgcc aacactgcac
    13261 agttttcccc caaaagatga aacatttggg gccattttga ctcatagagg aaagcatcgc
    13321 gcacaaactt tttcagttta tttgctggat taatattcta ttattatctt tatttataga
    13381 tttttatatt gcatgaggtg gtttttggag agaagaatga ggaagatgcg tcgagccaca
    13441 gaaacgttag ctttacata
//
LOCUS       DAZFKY010000129        13427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFKY010000129 DAZFKY010000000
VERSION     DAZFKY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..142
                     /locus_tag="ACHKCL_003349"
     CDS             <1..142
                     /locus_tag="ACHKCL_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5764247.1"
                     /translation="GKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADKV
                     RD"
     gene            complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHKCL_003350"
     CDS             complement(144..755)
                     /gene="emtA"
                     /locus_tag="ACHKCL_003350"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5764248.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            855..1769
                     /gene="ldcA"
                     /locus_tag="ACHKCL_003351"
     CDS             855..1769
                     /gene="ldcA"
                     /locus_tag="ACHKCL_003351"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5764249.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHKCL_003352"
     CDS             1865..3601
                     /gene="cvrA"
                     /locus_tag="ACHKCL_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5764250.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(3993..5063)
                     /gene="dadX"
                     /locus_tag="ACHKCL_003353"
     CDS             complement(3993..5063)
                     /gene="dadX"
                     /locus_tag="ACHKCL_003353"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5764251.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(5073..6371)
                     /gene="dadA"
                     /locus_tag="ACHKCL_003354"
     CDS             complement(5073..6371)
                     /gene="dadA"
                     /locus_tag="ACHKCL_003354"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5764252.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            6734..8266
                     /gene="ycgB"
                     /locus_tag="ACHKCL_003355"
     CDS             6734..8266
                     /gene="ycgB"
                     /locus_tag="ACHKCL_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5764253.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(8318..9037)
                     /gene="fadR"
                     /locus_tag="ACHKCL_003356"
     CDS             complement(8318..9037)
                     /gene="fadR"
                     /locus_tag="ACHKCL_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5764254.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            9259..10800
                     /gene="nhaB"
                     /locus_tag="ACHKCL_003357"
     CDS             9259..10800
                     /gene="nhaB"
                     /locus_tag="ACHKCL_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5764255.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10946..11476
                     /gene="dsbB"
                     /locus_tag="ACHKCL_003358"
     CDS             10946..11476
                     /gene="dsbB"
                     /locus_tag="ACHKCL_003358"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5764256.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(11522..12790)
                     /gene="umuC"
                     /locus_tag="ACHKCL_003359"
     CDS             complement(11522..12790)
                     /gene="umuC"
                     /locus_tag="ACHKCL_003359"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5764257.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(12790..13209)
                     /gene="umuD"
                     /locus_tag="ACHKCL_003360"
     CDS             complement(12790..13209)
                     /gene="umuD"
                     /locus_tag="ACHKCL_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5764258.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         3086 a         3536 c         3385 g         3420 t
ORIGIN      
        1 tggcaagaat gaaaccatca ccactccccg tctgagcttc cgttttctta acgtcagccc
       61 gacggtggag cggcaattac agcggattat tttctctctc gagcgggaag cccgggagaa
      121 agcggacaaa gtacgcgact gatttacatc gcgtccagtg cctgctcaag tttgtagata
      181 tagcgcggag cctgcggcgc aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca
      241 tccagatcgt tgattttgct gattgccttt ttccgatctg acgagaaagt ccgtagcagc
      301 gcacctgccc cgttagcata tgacaccacc agcgcatatt gcagtacctt cggatcttca
      361 atgcctgcca gcgggccggt ttccagaata ttcaggtaag ccgcccccat agaaatatta
      421 cgctccggat ttttcagctc gctggtcgtc ggctcaccac tccagcccat acgccgataa
      481 acatcacgtc cggaggttga agcttttaac tgcatcaaac caatggcatt cgatttactc
      541 accgcgttcg gattaccacc cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg
      601 acgccccagg ctgcaccggc tttctggctt attggcatcc actgcatcgc acgttgcacc
      661 ggaactttcg cgttccacgg cgggttcgta tagtcatgct ttgatgaaca acccgctaat
      721 aacacaatca aaaaggcaaa ccatctcaat ttcacttctt ctatccttat agcctggaat
      781 cgccgttgag gtgacaacgg cacacctaag cggcatgata tacatttcgt atctgttgtc
      841 agcaaggaat tgccatgtct ctgtttcact taattgcccc atcgggttac tgcattaaac
      901 agcacgccgc gctgcgtggt atccagcgcc ttaccgacgc ggggcatcaa gtcaataacg
      961 tcgaggttat tgcccgtcgt tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag
     1021 atctcaattc ccttgccaga ctgactaccc ccaacaccat cgtgctggct gtacgcggcg
     1081 gttacggtgc cagtcgttta ctggcagata ttgactggca ggcactggtg gctcgccaac
     1141 aatatgaccc gttgctcata tgcgggcata gcgattttac cgccattcag tgcggtcttc
     1201 tggcgcaagg caatgtcatc acctttagcg gccccatgct ggtagcaaac tttggcgcgg
     1261 atgaactgaa cacctttacc gagcaccatt tctggctggc gttacgcaat gaaaccttta
     1321 ccattgaatg gcaaggggaa ggcccgacat gtcgggccga aggcacgttg tggggaggca
     1381 atcttgcgat gctgatttca ctgatcggta caccgtggat gccaaaaatt gagaacggta
     1441 ttctggtgct ggaagatatt aacgagcacc ctttccgcgt cgaacgtatg ctgttgcagc
     1501 tctatcatgc gggaattttg ccacgtcaga aggcgattat tctcggtagc tttagcggca
     1561 gtacgcctaa tgattatgac gcaggttaca acctggagtc agtgtacgca tttttgcgtt
     1621 cccgcctgtc gattccactc attaccggcc tcgattttgg tcatgaacaa cgcacggtta
     1681 cgctgccgtt gggcgcacat gccattctta ctaacacccg ggaaggcact cagttgacga
     1741 ttagcggtca tcctgttctt aaaatgtaaa aaaaatcacg cttcagggct aagtaaaacg
     1801 ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca ggagtaaccg
     1861 accgttggat gccacaacaa taattagcct tttcatctta ggatccatcc ttgttaccag
     1921 cagtatatta cttagttcat tttcttctcg tcttggcatt cctattctgg ttatttttct
     1981 ggcgatcggc atgctggcgg gagtcgatgg cgtcggcggt atcccatttg ataattaccc
     2041 cttcgcctac atggtaagta acctggcgct ggcgattatt ttgctcgatg gcgggatgcg
     2101 cactcaggcc agttcctttc gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg
     2161 cgtacttatc acctctggtt taaccggcat gatggcggcg tggctgttta atcttgattt
     2221 gattgaaggc ttattaatcg gcgctatcgt cggctccacc gatgctgcag cggtcttttc
     2281 tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg
     2341 cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcgatga tccagcagca
     2401 tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg gcctcgggat
     2461 tgtcatcggt cttggcggtg gttatttact gctgcaaatg attaatcgca tcgccctgcc
     2521 cgccggttta tatccattgc tggcattaag cggcggtatt ttaatttttg ctttaaccac
     2581 ggcgctggaa ggtagcggta ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg
     2641 gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct ggctggcgca
     2701 aatcgccatg ttcctggtgc tggggctgtt ggttaaccca agcgatctgc tgcctattgc
     2761 cattccggcg ctcattttgt ccgcatggat gattttcttc gcccgtcctc tttcggtatt
     2821 tgccggattg ctaccattcc gtggcttcaa tctgcgtgag cgcgtgttta tcagctgggt
     2881 gggattacgc ggcgcggtgc cgattattct ggccgtgttc ccgatgatgg cggggctgga
     2941 gaatgcacgt ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca
     3001 gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg
     3061 tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc agtttgttta
     3121 tcaattgagt gccgataaat ggtgcgtggg cgcggcactg cgcgatttgc atatgccaaa
     3181 agagacgcgt attgcggcac tgtttcgtga taaccagttg cttcatccta ccggcagcac
     3241 ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc tcccggcgct
     3301 cggtaaactg ttcagccagt cgccgccggt cgcgctggat caacgcttct ttggtgactt
     3361 cattctcgaa gccagcgcca aatatgcaga tgtggcgctg atatatggcc tggaagacgg
     3421 gcgagaatat cgtgataagc aacaaacgct gggtgaaatt gtccagcagt tgttaggcgc
     3481 ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg tggccgagaa
     3541 agaagataat gaagtgttga agattggcgt tcgagtggct gaagaggaag ctgaatctta
     3601 aatttaatga agcatctatc gccggttacg atgctttgct taacgcagcc tgagcctgta
     3661 tataaacttg tcatttcctg attttgatat gcacattcca acatcagaag aagcagcctg
     3721 ttaatcaaca ttctgaacat gaacccgaat ctgcatcaag ggtgaagcgt gatttatctg
     3781 aagtcgagtt cgagtcaaca atgaagcgta agcgttttat gtagaacgcc tgtgcggtga
     3841 gttcaggttc cggggaaata gtgtccatcc ttttaatggg cactatcatg ggcaatggct
     3901 ctgtcacatt aacaactaca gttgctgacc agccggatgg atgatttttt ttgcaaccag
     3961 aagacgttgc ctccgatccg acttacaaca agttacaccg tcacaaccgg gacgcgtaac
     4021 gccagcgcgc acatcaactc atagcccacc gttccggcag cggcggcgac atcatcaatt
     4081 ttgatctcct tgccccacag ctcaactggc gtaccaatac ccgcctgcgg gcaaggcgtt
     4141 aaatcgaccg ccagcatatc catcgagact gtccccaccg tcatggtgcg cacgccgtcc
     4201 actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta tcctgcggca
     4261 acaatgccga ttcgctgctc atcgcgcgca gtataacgac cgccgtagcc cacacgctcg
     4321 cccgctttta gcgtctggac accaataatc tcactgctta gcgtcatcac cggacgtaat
     4381 ccagtattgg cgatatcacg ccactgaccg gacggcgaag cgccatacaa aataatgcca
     4441 ggccgaaccc agtcaaaatg cgcttctggg tgccacagag tcgccgccga attggacaac
     4501 gaacgccgac actccagccc ttccgccgcc tgctcaatac gcgccatcgc gccggaaatt
     4561 ccatcaggat gttccgcctc ggcaaaatgc gacatcaggg tcatttcgcc aacattcgcc
     4621 atcgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc caaccgattc
     4681 atcccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc attttgcagt
     4741 gctttgagct gccagttgct gtgtacgcag gtggtcaggc ggtgctggtc atacatttcc
     4801 agatcctgag catggaaaaa tccttccagc atcaggatcg gtcctttcca gccgcgctca
     4861 cgtaacgtta ttgcttcttc caggttaagt aatgcaaagc catcggtagc cccgagcgcg
     4921 ctccagatac gctcaatacc gtgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc
     4981 gcgtgcggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg cagatcgagg
     5041 ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg taaatggccc
     5101 ggacgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagatcctc atatgggatc
     5161 gctggcgtgc gaccagagag cagatcgctt aacaactgac cggaaccgca agccatcgtc
     5221 cagccgagcg tgccgtgacc ggtatttagc cacagatttt taaagcgtgt acgcccgaca
     5281 accggcgtgc cgtctggcgt catcgggcgc agacccgtcc agaaagtcgc ctgctcgaca
     5341 tgaccgccgc gtggatagag atcgcgaacc accatctcca gcgtttcacg acgcggttgc
     5401 aacagctcgg tattaaaacc aacaatttcc gccattccgc caacacgaat gcggttatcg
     5461 aaacgggtaa tggcgatttt gtaggtttca tcaagaatgg tggataccgg cgcaccatct
     5521 tcctgcgcaa ttggaatggt cagcgagtag cctttcagcg gatacaccgg aatatcaaca
     5581 atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc atcggcctta
     5641 atcacttcat cgccacactt cacgccgtag atttgctcgc cgtcgcaaag cagttggtca
     5701 acgggcgtat taaagcggaa tttaaccccc gcctgctccg ccatccgcgc cagattctgg
     5761 gtaaacagct ggcagtctcc ggtttcatca ttgggcaatt gcagaccacc cgtcagtttg
     5821 tgcgctactt ctgccagcgc gggctccact tccgccaggc ggctggattc cagcagctga
     5881 tacggtacgc cggcatcttc cagcacggcg atatcgcggg tcgcattttc atactgttgt
     5941 tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat attggtttcg
     6001 gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgcaccat ccgccctttg
     6061 ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca tttcaactgg
     6121 aactgcgtac cgtcgagacg aaccgccagc ggcgcatggc gctggaacat ccatttaatc
     6181 gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga gatttgcccg
     6241 gcattcgcgg cactggtttc cagggcggcc cccggctccc gatcaatgac ggtgacctca
     6301 tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact tcccagtatg
     6361 acaactcgca tagccactcc gataacggta gatacagtaa aagaataata atctaattac
     6421 atcttgataa ctcagttgaa aatattattc aacatacggc ttttttatgg tgagctggct
     6481 cacatctccc tgactctgcg gcaatatggc gtctttagta tctcctgctg aacgcccttt
     6541 ttatccagca taaaattccg ttcagaaacg gattagtggc actctgataa taagaaatcg
     6601 caaagaaaaa aattctccgg cgacgcggtt tttaacaggt gttctatgct tgaaatgagg
     6661 tgctccacac agagagcgcc gacaacaatg aggtgctcca cacagagagc gccgacaaca
     6721 atgaggtgcg cgtatggcga cgatcgattc tatgaataag gacaccacac gtttgagcga
     6781 tggacccgac tggacgttcg acctgctgga tgtttatctg gcagagatag accgggtggc
     6841 gaaactctac cggctggata cctacccgca ccagattgaa gtgataacct cagaacagat
     6901 gatggatgcc tactccagcg tcggcatgcc aattaactat ccgcactggt cattcggtaa
     6961 aaagtttatc gagactgaac ggctgtataa gcacggtcag caaggactgg cctatgaaat
     7021 cgtcattaac tctaacccgt gtatcgctta cctgatggaa gagaacacca ttaccatgca
     7081 agcgctggtg atggctcatg cctgctatgg gcataactct ttcttcaaaa acaattactt
     7141 attccgtagc tggaccgacg ccagttcgat tgtcgattat ctgatttttg cccgtaaata
     7201 tattaccgag tgcgaagaac gttatggcgt tgatgaagta gaacggcttc tggactcgtg
     7261 ccacgcgctg atgaactacg gcgtggaccg gtacaaacgc ccgcaaaaaa tctcgctgca
     7321 agaagagaaa gcccggcaga aaagtcgcga agagtatttg caaagtcagg tcaatatgct
     7381 ctggcgtacc ctgccgaagc gcgaggaaga gaaaacggtt gctgaagcgc gccgttatcc
     7441 gtccgaacca caagaaaacc tgctctattt tatggagaaa aatgcgccac tgctggaatc
     7501 atggcagcgt gaaatcctgc gtattgtgcg taaggtgagc cagtattttt atccgcaaaa
     7561 acagactcag gtgatgaacg aaggctgggc caccttctgg cactacacca tccttaacca
     7621 tctgtatgat gaagggaaag taacggaacg ttttatgctg gagtttttgc acagccacac
     7681 caatgtggtc ttccagcccc cctataacag cccgtggtac agcggcatca acccgtatgc
     7741 cctcgggttc gccatgttcc aggatattaa acggatttgt cagtcgccaa cggaagaaga
     7801 caaatactgg ttcccggata tcgccggttc cgactggctg gaaacgctgc atttcgcgat
     7861 gcgtgatttc aaagatgaga gttttatcag ccagttcctg tcgccgaaag tgatgcgtga
     7921 tttccgcttc ttcaccgtgc tggatgatga tcggcataat tatctggaga tttccgctat
     7981 tcataatgaa gaaggttatc gggagatccg taaccggcta tcgtcgcaat ataacttaag
     8041 taatctggag ccgaatattc agatctggaa cgtggatttg cgcggcgacc gttcgctgac
     8101 gctgcgttac attccacata atcgcgcacc gctggatcgg gggcgcaaag aagtactgaa
     8161 gcatgtgcat cgcctgtggg gatttgatgt gatgctggaa cagcaaaacg aagacggcag
     8221 cgtcgagttg ctggaacgtt gcccgccgag aatgggaaat ctgtaaaaac aacaaaaaac
     8281 ccctcgtttg aggggtttgt tttttaaacg gaagggatta tcgcccctga atggctaaat
     8341 cacccggcag atttttctgc atccggtgcc aaatctcgcc actctcatgc ccatagcgac
     8401 gcactgtttc gtacacctga tcgtgcgcgc cttcactgca caacgccgac agtttgtggt
     8461 agaagcccag cgccagactg cgcgcttccg gattggcgaa atagtgacga ccaatgcgcg
     8521 tatacagtcc cttcatcccg ttaaggatca gaccgtaaat cgggttgccg gaagcaaacg
     8581 ccaggccgcg gaatatgttg taatccagct cggcaaaggc atcggcgtga tcggccactt
     8641 cattagcggt agccagcact tcctgcgctt tatcgggatg ctgacgaaac gcggtgcgaa
     8701 taaaaatagt tgaaatattg gtacgcaccg acagcaaatt atcaataagc tgcggcacac
     8761 tttcgtgatc cagtcgcgcc agtgtttcaa ggatatttaa accagaggtt tcccagaaat
     8821 tattcacctt cgtcggcttg ccatgttgaa tggtcaacca gccatctcgt gccagacgct
     8881 gtaacacttc acgtaacgta gtacgcgtta cgccaattaa ttctgaaagt tcacgttctg
     8941 cgggcaaaat agtcccggga gggaagcggt tattccagat actttcaata atgtactctt
     9001 ccgcgaaacc cgccgggctt tgcgccttaa tgaccatagt gagatttcca taacacagca
     9061 aaacaaagtt ggactcatca taccagaggg ctaactacgc tgatagcaga caaaaagaag
     9121 aaaaaaggga tcaaggcttc attttccgca tctctttccc cttgactatc aagacttgcc
     9181 tcactcctgc ctgaccgtta agctttgcgt ttaaacataa aaacatcatt gattatttgt
     9241 aaggtaaggg aaaccatcat ggagatctcc tggggccgcg cgctatggcg caattttttg
     9301 ggccagtccc ccgactggta caaactcgcc ctcatcattt tcttaatcgt aaatccgtta
     9361 attttcctca tcagcccttt cgtcgctggc tggttgctgg tagcggaatt tattttcact
     9421 ctggcgatgg ccctgaaatg ctacccgctg ctccccggtg gcctgttggc tatcgaagcg
     9481 gtattcatcg gcatgaccag cgcggaacac gtccgtgaag aggtggcggc aaatcttgaa
     9541 gtcttgctgt tactgatgtt tatggtggcg ggtatctatt ttatgaaaca gctattgctg
     9601 ttcatattta cccgtttgct gttaagcatt cgctccaaaa tgctgctgtc gctctctttt
     9661 tgcgtagcgg cagcgttcct ctccgcgttc ctcgatgcct taaccgtcgt ggcggtggtg
     9721 atcagcgttg ccgtcggttt ttatggtatt tatcatcgcg tagcctcttc ccgtaccgaa
     9781 gacaccgacc tgcaagacga tagtcatatc gacaagcatt acaaagtggt tctggaacag
     9841 ttccgtggct ttctgcgtag cctgatgatg catgccggtg tcggcaccgc attaggcggc
     9901 gtaatgacca tggtgggcga accacagaac ctgatcatcg ctaaagcggc tggctggcat
     9961 tttggcgatt tcttcctgcg catgtcgccg gtgaccgttc cggttctgat ttgtggcctg
    10021 ttaacctgcc tgctggtaga gaagctgcgt tggtttggct acggtgaaac gctgccggag
    10081 aaagtccgcg aagtgttgca acagtttgac gatcaaagcc gccaccagcg tacccgtcag
    10141 gataaaatcc gtctgattgt ccaggcgatt attggcgtct ggctggtgac tgcgctggcg
    10201 ttgcatctgg cggaagttgg cttgattggt ttgtcagtca ttattctggc aacatcattg
    10261 accggtgtca ccgatgaaca tgctatcggt aaagccttca ccgaatctct gccattcacc
    10321 gcactgttga cggtgttttt ctcggtagtc gcggtgatta tcgaccaaca actgttttcg
    10381 ccgattattc agtttgtgtt gcaggcatcg gaacatgctc agctgtcgct gttctatatt
    10441 ttcaacggtc tgctgtcatc catttcggat aacgtcttcg tggggacgat ttatatcaac
    10501 gaagcgaaag cggcaatgga aagtggcgct atcacgttga agcaatacga gctgctggcg
    10561 gtcgccatta ataccggtac caatctgccc tctgtcgcta cgccgaacgg tcaggctgcg
    10621 ttcctgttcc tgctgacctc tgcactcgcg ccattgattc gcctctctta tggccgcatg
    10681 gtgtggatgg ccctgcctta caccctcgtc ctgacactcg tcggactgct ctgcgtcgag
    10741 tttacgcttg cccctgtaac cgaatggttt atgcaaatgg gctggatagc aacgctttga
    10801 taacaactta ccgggcatat aaatgcccgg tttgcctttt cgcccgataa ttgtccaatt
    10861 gcgtcctatt tttgctgcct cctggtggcg cagcgaatga attggtttaa actgcgcact
    10921 ctatgcatat tgcagggaaa tgattatgtt acgatttttg aaccaatgtt cacaaggccg
    10981 gggcgcgtgg ctgttgatgg cgtttactgc tctggcactg gaactgacgg cgctgtggtt
    11041 ccagcatgtg atgttactga aaccttgcgt gctctgtatt tatgaacgct gcgcattatt
    11101 cggcgttctg ggtgctgcgc tgattggcgc gatcgcccca aaaacgccac tgcgttatgt
    11161 agcgatggtt atctggttgt atagtgcgtt ccgcggtgtg cagttaactt acgagcacac
    11221 catgcttcag ctctatcctt cgccgtttgc cacctgtgat tttatggttc gtttcccgga
    11281 atggctgccg ctggataaat gggtgccgca agtgtttgtc gcctctggcg attgcgccga
    11341 gcgtcagtgg gattttttag gtctggaaat gccgcagtgg ctgctcggta tttttatcgc
    11401 ttacctgatt gtcgcggtgc tggtggtgat ttcccagccg tttaaagcga aaaaacgtga
    11461 cctgttcggt cgctaatcca ttcggcgctc ctgcgggagc gcttttttcc tgccgctgtg
    11521 tttatttgac ccgcagtaaa tcagaacttc gcgttgtata acgtggtgaa agcatggctc
    11581 gcttcatctg ccattgttgc tggatcccct gcccggcaaa atagagtgtt cctctgccct
    11641 ctttagcatt cagtgtatcc attaccgcca tcaattgctc actcccgggg cgcggagcgt
    11701 tgtcatcgaa taaattgagc tgcgcgactc cctgactgaa gaaatccccc agcatcacgc
    11761 ccgctttttg gtagcgatgg cccgcttgcc agatggcatc cagagatcgc gtagcggcgt
    11821 taatgatatc cctgctgtcc tgagtgggcg tcagcagttt taccgacgcg ctattgccgt
    11881 aataaggttc attgagcgca aatggtgacg tcttaataaa tgtggagata aaccgacaat
    11941 attgatgctc actgcgaagt ttttccgccg cccgggcagc gtaactacaa atggcctgcc
    12001 gcatcgacgt ataatccgtg atgcgttcac caaacgagcg ggaacagata atttcctgct
    12061 ttgtcggtgc aaattcttcc agttgcaaac agggttcacc gcgcagttca cgcaccgttc
    12121 tttcgagcac gacattaaaa tgtttacgga taaaccggat atctgtatcc gccaaatcga
    12181 gaacggtttt gatccccatc gcgtccagtt ttttgctgat ccgccgtcca atcccccaga
    12241 cgtcatccac ggggagagca gacattaatt tacgctggcg ttccagattt gataaatcca
    12301 ccaccccacc cgtctgccgc tgccattttt ttgccgcatg attggcaagc ttagccagcg
    12361 ttttggtctg ggcgatcccc acaccaacag taagatgggt acgttgtagc accgttgcgc
    12421 gaatttctct gccaaagtca gtcagatcgc gacaattacg cacacctgtc agatcgcaga
    12481 atgcctcatc aatactgtaa atctcgacgc ggggcgatag ttcttccagc gtcgacatca
    12541 cccgattgct catgtctgcg taaagctcat aattgctgct aaagcaaacc acgccacagc
    12601 gacgaaacag atctttttgt ttgaaccagg gatcgcccat tttaacgcca agcgcctttg
    12661 cctcagcgtt tcgggcgata acgcaaccgt cattattcga tagcacaacc accggtttac
    12721 cccataactc agggcgaaac accgtctcac agctggcata aaacgcgttt acatcacaga
    12781 gggcaaacat cagcgcatcg ccttaacgac gtggatcacc acaccaaaga catccagcgt
    12841 atcttcacta ctgatggtaa tgggcgagta cgcgctgttc atgggaataa gctgtaccgt
    12901 cgggcgtagt tgcaattttt tcaccgtaaa ctcgccgtca acagcagcga tgacaatatc
    12961 accatggctg gcggtaatag cgctatcgac aatcagtaaa tcaccgtcac taattccacc
    13021 atcaatcata gaatcaccac ttgctttgac gaagtaagtc gcgctgggat gctggatcaa
    13081 cagttgattc agatcgatgc gctgttcaac gtaatctgcg gccggtgaag gaaagccaca
    13141 ctgaacaaga tcgctaaata gcggaaaagt cacaatttcg cggagatccg caggcttgat
    13201 aaacaacata ataatctgcc tgaagttata ctgtttttat atacagtagt ctgttcttgc
    13261 cagcagatca atactgattc aggctatcaa tatttgtcgc tgcatagact gctgattttt
    13321 cgttctctta tcctgtgctc acgcggcctt ctggcgacga cgctcatcca gcagaaatga
    13381 aaaatatcac ccggctaaaa aatagaatag aagcattcgc cataatg
//
LOCUS       DAZFKY010000130        13378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFKY010000130 DAZFKY010000000
VERSION     DAZFKY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            263..1360
                     /gene="mltA"
                     /locus_tag="ACHKCL_003361"
     CDS             263..1360
                     /gene="mltA"
                     /locus_tag="ACHKCL_003361"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5764259.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1599..2405
                     /gene="tcdA"
                     /locus_tag="ACHKCL_003362"
     CDS             1599..2405
                     /gene="tcdA"
                     /locus_tag="ACHKCL_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5764260.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2456..2899)
                     /gene="csdE"
                     /locus_tag="ACHKCL_003363"
     CDS             complement(2456..2899)
                     /gene="csdE"
                     /locus_tag="ACHKCL_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5764261.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2899..4104)
                     /gene="csdA"
                     /locus_tag="ACHKCL_003364"
     CDS             complement(2899..4104)
                     /gene="csdA"
                     /locus_tag="ACHKCL_003364"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5764262.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4296..4523
                     /gene="ygdI"
                     /locus_tag="ACHKCL_003365"
     CDS             4296..4523
                     /gene="ygdI"
                     /locus_tag="ACHKCL_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5764263.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4874..5791
                     /gene="gcvA"
                     /locus_tag="ACHKCL_003366"
     CDS             4874..5791
                     /gene="gcvA"
                     /locus_tag="ACHKCL_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5764264.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5810..6205
                     /gene="ygdD"
                     /locus_tag="ACHKCL_003367"
     CDS             5810..6205
                     /gene="ygdD"
                     /locus_tag="ACHKCL_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5764265.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6198..7298
                     /gene="rlmM"
                     /locus_tag="ACHKCL_003368"
     CDS             6198..7298
                     /gene="rlmM"
                     /locus_tag="ACHKCL_003368"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5764266.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7342..8073)
                     /gene="fucR"
                     /locus_tag="ACHKCL_003369"
     CDS             complement(7342..8073)
                     /gene="fucR"
                     /locus_tag="ACHKCL_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5764267.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(8131..8553)
                     /gene="fucU"
                     /locus_tag="ACHKCL_003370"
     CDS             complement(8131..8553)
                     /gene="fucU"
                     /locus_tag="ACHKCL_003370"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5764268.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8555..9973)
                     /gene="fucK"
                     /locus_tag="ACHKCL_003371"
     CDS             complement(8555..9973)
                     /gene="fucK"
                     /locus_tag="ACHKCL_003371"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5764269.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQFRYFYPQTEPEFIEEV"
     gene            complement(10082..11857)
                     /gene="fucI"
                     /locus_tag="ACHKCL_003372"
     CDS             complement(10082..11857)
                     /gene="fucI"
                     /locus_tag="ACHKCL_003372"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5764270.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11890..13206)
                     /gene="fucP"
                     /locus_tag="ACHKCL_003373"
     CDS             complement(11890..13206)
                     /gene="fucP"
                     /locus_tag="ACHKCL_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5764271.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3223 a         3321 c         3530 g         3304 t
ORIGIN      
        1 ccgacgacct tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgtccgtg
       61 gatgcgcact atactccgct cgcgttttca tgcaaccttt tttttacccc gatcacggat
      121 ctggcatgat tttgaacaaa aacaaatcac taaagcctat tttttgtgca aaatttgcgt
      181 cagggagagg ctgctcattt cattaaacgc ggtggtttgt tatcttcgtt gcgccttatt
      241 ttttaacctg aagaagagaa caatgaaagg acgttgggta aagtaccttc ttatgggcac
      301 ggttgtggca atgcttgccg cctgctcttc caaaccaacc gatcgcggac agcaatataa
      361 agacgggaaa tttacccagc ctttctctct ggtgaaccag ccagatgccg ttggcgcgcc
      421 gattaacgcc ggtgattttg ccgagcaaat taaccatatc cgtaattcgt caccgcgtct
      481 gtatggcaac cagagtaatg tttataacgc ggtgcaagag tggctgcgcg caggcggtga
      541 tacccgcaat atgcgccagt tcggcattga tgcctggcag atggaaggtg ccgacaacta
      601 tggtaacgtg cagtttacag gctattacac gccggtaatt caggcgcgcc atacccgcca
      661 gggcgagttc cagtatccta tttaccgtat gccgccaaaa cgtggtcgtc tgccgtctcg
      721 tgcggagatc tacgcgggtg cattgagcga taagtacatc ctcgcttaca gcaactctct
      781 gatggataat ttcattatgg atgtgcaggg tagtgggtat atcgactttg gtgatggcag
      841 tccgcttaac tttttcagct atgcagggaa aaacggtcat gcctatcgca gcattggtaa
      901 ggtgctgatc gaccgtggcg aagtgaaaaa agaagatatg tcgatgcagg cgattcgtca
      961 ttggggcgaa acacacagtg aagccgaggt tcgcgagctg ctggaacaga acccgtcttt
     1021 cgttttcttt aaaccgcaat cttttgcacc ggtgaaaggg gcaagtgcgg tgccgctggt
     1081 tggccgtgct tctgttgcct ctgatcgttc cattatccca ccaggtacaa ccttgctggc
     1141 agaagtgccg ctgctggata ataacggcaa atttaacggt cagtacgaat tgcgtctgat
     1201 ggtggcgttg gatgtcggtg gcgcaatcaa aggccaacac ttcgatattt atcaagggat
     1261 cgggccggaa gccggacacc gcgcaggttg gtacaaccac tatggacgtg tctgggtgct
     1321 gaaaaccgcc ccgggcgcag gtaacgtctt tagcggctga tgtgatattc tgtatgccgt
     1381 ttttacggat atgacagggt gaggggtaac ctcacatcct gattattgac taagtgcgcg
     1441 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcatg caaatccaaa
     1501 atattgcagg agttatgtag gcctgataag cgtagcgcat caggcattta tgcgtttagg
     1561 gtgagggata acctcgccct ttttaattct gaggtgctat gtctgtggta attagtgatg
     1621 catggcgtca gcgttttggt ggcacagcgc gtctgtatgg tgaaaaagcg ttgcaactgt
     1681 ttgctgacgc gcatatttgt gtggtcggta ttggcggtgt cggttcctgg gcggcggaag
     1741 cgctggcgcg cacggggatt ggcgcaatca cgcttatcga tatggatgat gtgtgcgtca
     1801 ccaataccaa tcggcaaatt catgccctgc gcgataacgt tgggctggca aaagcggaag
     1861 tcatggcgga acgtattcgc cagattaact cggagtgctg tgtaacggtg gtggatgatt
     1921 tcgtgacgcc ggataacgta gcgcagcata tgagcgtggg ttattcgtac gtgattgatg
     1981 ccattgatag tgtacggccc aaagcggcgc tgattgctta ttgtcggcgc aataaaatcc
     2041 cgctggtcac gaccggtggc gcgggtgggc agattgaccc aacgcagatt caggttaccg
     2101 atctggcgaa aacgattcag gacccgctgg cggcgaagtt gcgtgagcgc ctgaaaagcg
     2161 attttggcgt agtgaaaaac agtaaaggta agctcggtgt ggattgcgtg ttctctaccg
     2221 aagcattggt gtatccgcag tcagacggta cggtgtgtgc gatgaaagcc acggcggaag
     2281 gaccaaagcg gatggattgt gcatctggat ttggcgcggc aacgatggtg accgccacct
     2341 ttggttttgt tgcggtttct catgcgctga agaagatgat ggcgaaagcg gcgcgtcagg
     2401 ggtaaacaat caggtcggat gcgacgcaaa cgtcttatcc gacccggttc tgtgcttaaa
     2461 cctgcttcgt cgcagcgata atcgcctcgc ttaacgcatt taacccctgg ctgcgtgagg
     2521 cgctaagttg cgcacgcaat cccagctcat caaacaatgc cagcggcgac tgtgcctgca
     2581 attcggcggc ggttttcccc tcaacggcag tcaacagcac cgccaggagg ccgcgcacta
     2641 tacgtccttc gctgtcgcca aagaagtgca ttttaccgtt ttcagccacg gtatatccca
     2701 gccagacgcg gttttcgcat ccggcaatct ctttagcctg cgcttttaac tcgtctggca
     2761 aagccggaag ctgtttcccc agcatgatca actggcgata tttatcttcc cattgcgtca
     2821 acggtgcgaa ggtattgcgt aacgtttctg cggttacggt tgtgccgaac ggatgtccgg
     2881 cgaattgcgg gttcgtcatt aatccaccaa taattccagc gcgcggtcaa cggcattcac
     2941 cagcgcatcc acatcactct ttgtattata tggcgcaaaa gaggcgcgca gtgtgccggt
     3001 cacgcctaat tctgccagta gcggctgagc gcaatgttgc ccggcccgca aggcaatacc
     3061 gtactccgcc agcagcgtca ccatatcgct gtgatgaacg ccagcaaaat caaaggccag
     3121 caggctggaa tcctggcagc ggaatgaacg aaagcctgga cgtttcgcca gcgcatcttc
     3181 cgccagcgtt gctaagctac ggctccagtt ttcggcctgg ttgatatcgt aatctgccag
     3241 ccattccagc gccgcgctta atcctatgac accagccaca tttggcgttc ctgcttccag
     3301 tttccacggc gcagattgag tcgtgaagcc gtcaaaactc acttcgtgaa ccattttgcc
     3361 gccgcccagc cagggcgaca tcgcttccag cagttctgat ttgccataca gcacgccgat
     3421 acccgtcggg ccatacagtt tgtgaccaga gaaagcatag aaatcgatat ccagttgctg
     3481 aacatccgcg gggaaatgca ccgccccctg agcaccatca actatcacca ctatcccggc
     3541 tgaatgagca aaggtaatcg ctcgcgccag atccgggcaa ccgccagtta cgttcgacat
     3601 ctgacccaac gccagaatcc gactacgggg agtaatcagt tccggcagca aatcgacatc
     3661 tggcagtcgc tgcgcattaa gcggcaattt gaccactttg gctccagttt gttgggcgac
     3721 catcagccag gggacgaggt tggcgtggtg ttctgccacg ctgacaataa tctcatcgcc
     3781 cggttgcaga cgcggacgcg catagcattg tgccaccatg ttgatggatt cagtggtgcc
     3841 gcgcgtccag acgatagttt tatcatccgg tgcattcagt aattgcgcca ctttctctcg
     3901 tgcagcttca taacgcgcgg tcaggcgttg ggcttcggca aactggctgc gatggacgtt
     3961 tccggcgctc agactgtaaa actgtcgggt ggcttcaacc acggcttcag gtttaagcgc
     4021 ggtcgcggcg ctgtcgagat agacgcccgc atcctgtaga gcgggaaact gggcgcgaaa
     4081 ctgcgcggga ttaaaaacgt tcatggtact cctcggcttg atgcaccgat cgtgacgcaa
     4141 aattcacttt gatacaagga gagtgctaac catcagaatt atctggatat cctggcgaag
     4201 ccgcgtcagt aacatatgct gaatagtgtt aggcgttaat tttagcctgt taatcaatac
     4261 tattaagatt tattaatcgc tttaaggaga agatgatgaa aaagactgcc gcaattattt
     4321 ctgcctgtat gctgactttt gccctgagcg cctgttccgg ttcgaactat gtgatgcaca
     4381 ccaatgacgg acgtaccatc gtctctgacg gcaaaccaca gactgataac gataccggta
     4441 tgatttcgta taaagacgct aatggcaaca aacagcagat caaccgtacc gacgtgaaag
     4501 agatggtcga actggatcag taattcgcga tcgcaaggta aaaaaaagca ccgcaattag
     4561 gcggtgctac attaatcact atggacagac agggtaaatg tacaggaagt gaaaaaaggt
     4621 agctttgcta ccatggtctg aatcgcagac caattgcaaa cacaacaaca caacatcaca
     4681 accgtaagcc aaaagttcac cagaacacgc attccgataa aacttttcgt tccggctcag
     4741 gaagtgccgc cactataggt atttgctggt agaagctcaa cggacaattt ataatggctc
     4801 agattaaaaa aactaatagg ttacacagtg tgatctaatt gttaaattca tttaacatca
     4861 aagtttaaaa gccatgtcta aacgattacc accgctaaat gccttacgag tttttgatgc
     4921 cgcagcacgc catttaagtt tcactcgcgc agcagaagag ctttttgtga cccaggccgc
     4981 agtaagtcat caaatcaagt ctcttgagga ttttttgggg ctaaaactgt tccgccgccg
     5041 taatcgttca ctcctgctga ccgaggaagg gcaaagctat ttcctcgata tcaaagagat
     5101 attttcgcaa ttaaccgaag cgacgcgtaa actccaggcc cgtagcgcca agggggcgtt
     5161 gacggtcagt ttactcccca gtttcgccat tcattggttg gttccgcgac tttccagctt
     5221 taattcagct tatccgggaa ttgacgttcg aatccaggcg gttgatcgtc aggaagataa
     5281 gctggcggat gatgttgatg tggcgatatt ttatggtcgg ggcaactggc cggggctacg
     5341 ggtggaaaaa ctgtacgccg aatatttatt gccggtgtgt tcgccgctac tgctgactgg
     5401 cgaaaaaccc ttgaagaccc cggaagatct ggctaaacat acgttattac atgatgcgtc
     5461 acgccgtgac tggcagacat atactcgtca attggggtta aatcatatca acgttcagca
     5521 agggccaatt tttagccata gcgccatggt gctgcaagcg gctattcacg ggcagggagt
     5581 ggcgctggca aataacgtga tggcgcaatc tgaaattgaa gccggacgtc ttgtttgccc
     5641 gtttaatgat gttctggtca gtaaaaacgc tttttatctg gtttgtcatg acagccaggc
     5701 agaactgggt aaaatagccg cctttcgcca atggatcctg gcgaaagccg ctgctgaaca
     5761 agaaaaattc cgctttcgtt atgaacaata atttacgtag ggtacgacca tgaccagccg
     5821 ttttatgctg attttcgccg ccattagcgg cttcattttt gtggcactgg gcgcttttgg
     5881 cgcgcatgtg ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc agaccggcct
     5941 cgaataccag gcgtttcata cgctggcgat cttaggtctg gcggtggcaa tgcagcgtcg
     6001 catcagtatc tggttttact ggagtagcgt tttcctcgcg ttaggcacgg tgctgttcag
     6061 cggcagcctt tattgcctgg cactgtccca tctgcgtttg tgggcgtttg tcactccggt
     6121 tggcggcgtg agcttccttg cgggctgggc gttaatgtta gttggtgcta tccgtttaaa
     6181 gcgcaagggc gtaagtcatg aataaggttg tattgctgtg ccgtccgggc tttgaaaaag
     6241 agtgcgccgc agaaattacc gataaagccg gccagcggga gattttcggt tttgcccgcg
     6301 tgaaagagaa tgcgggttat gtcatttatg aatgttatca acctgatgat ggcgataagt
     6361 taatccgtga gctgccgttc agttcattaa tttttgcccg ccagtggttt gtggtggggg
     6421 aactcttgca gcatttgccg ccagaagatc gtattacccc cattgtcggc atgttgcagg
     6481 gcgtagtaga gaagggcggt gaactgcgtg ttgaagttgc cgataccaac gaaagcaaag
     6541 agttgctgaa attctgccgt aaattcaccg tgccgctacg cgctgccttg cgcgatgccg
     6601 gggtgctggc gaactatgaa acgccgaagc gtccggttgt gcatgtattc tttattgcac
     6661 caggctgctg ctataccggt tactcataca gcaacaataa ttcgccgttc tatatgggca
     6721 ttccgcgcct gaaatttccg gcagatgcgc cgagtcgttc cacgctcaaa ctggaagagg
     6781 catttcatgt gtttattcct gcggatgagt gggatgaacg cctggcgaac gggatgtggg
     6841 cggtggattt aggcgcttgc cctggcggct ggacctacca actggtgaag cgcaacatgt
     6901 gggtttattc cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc ggacaggtga
     6961 cgtggctgcg ggaagacggt ttcaaattcc gtccgacgcg cagcaatatc tcctggatgg
     7021 tatgcgatat ggttgaaaaa ccggcgaaag ttgcggcatt gatggcgcag tggctggtta
     7081 atggctggtg tcgtgaaacc attttcaacc tcaaactgcc gatgaaaaaa cgctacgaag
     7141 aagtgtcaca caatctggcg tatattcagg cacagcttga tgaacatggc ataaatgctc
     7201 agattcaggc gcggcagttg tatcacgatc gcgaagaagt gacggtgcac gtccgccgta
     7261 tctgggctgc ggtgggtggt cgtcgcgacg agcgataaca ggcaaggccg gggcgtcgtc
     7321 tccggcctgc taccctttcc gtcagcctgt taccaaagaa gttgcaacct ggcgttcatc
     7381 agaaataatg tgcgttacct catccagatg cccgatgcgg gcttcccctg aacgattaaa
     7441 tttactttta tcaatcaata acaacgattg cgcggcgcgt tttaatagca ttgatttgta
     7501 atcagcgttg atagcattgg agtcccacag tgcgccgctg ctatcgatcc cttcacaaga
     7561 aaaaataaac agatcgattt ccagcgattt aagttgggaa atcagcgagg gattgacgta
     7621 acagccatat ttgcgctcaa gtgtgccgcc ggaactgatc agttgaatgc gttcgcgttt
     7681 accgagttca tggcaaatcg gatggctatt ggtgaagacc tgaatgttga tgtcaggcaa
     7741 ctggcgtgcc agataccagc aagttgaact ggcgtctaag gctatcacca tcccttcttc
     7801 aatccacgcg agcgcctcgc gcgcgatatc tgctttatgc gcataatggc ttttcagcct
     7861 gatgtgaaag ggatcgccac tgtcttgatt ttgacggtgg atatatttag cgcgtccatg
     7921 attgcgcaga attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa tggtttcttt
     7981 actgaccttt agctgttcag agagagcttc cgtggtcagg ctggtatggt tcagcagcag
     8041 gtcgactatc gcttgctggc gtgccgcttt catatttttc accccatcat tcgataaccg
     8101 cccgcgtcat gcgggcgtac cggtatgaga ttacggtgtt accccttttt ttaaaagaat
     8161 attcccgtac ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt gcgcccgttc
     8221 ataaaacgca aaacgattga tgcggatgat gtcaggacac ggggcttgta gtgaaagcgc
     8281 attacggtaa cgtcgttcta cttcaggatc gagagtgtca ccttctaccg ctgccatcat
     8341 caccagcggc ggtgcataac tgtccagctc aaataacggg ataatcgcct ggagcaagtc
     8401 gctcaccaac aggccatcag cgcggatcac ctgcggtccc atcgaatggg cgggaaagtg
     8461 agcatcggaa aaaataattt catctccatg tcccatctct gccagcactt tcaatagttc
     8521 gggagaaatt aacggcgaaa ttgttttcag catttcacac ttcctctata aattcaggtt
     8581 cagtttgcgg gtagaaataa cggaactgat aatgaatctg tgcacgggct tcttccgggc
     8641 tgttaaattc ccctacgcca taccaaccga acagcgcagc tcctgcgacg gtcgtttcgg
     8701 cgtcgtcgag aacttttaac ggaatatcaa gcatattggc tttaatctga ttccacaatg
     8761 tgttgcgact tcctccaccg actaacaata attcagaggc cttaaagtgc ccgatttttt
     8821 ccagcatctg tagattgcgc tgtaattgcg cagttaaccc ttccagcgcc gcgcgataga
     8881 aatgcccccg cgtggtatta agcgtcactc cttgccagcc agcgttctga cacgacaata
     8941 aatcacactg cattttcacg ccatccgcgc caggcgcgat cagacgagcc tcttcaatca
     9001 acatttgcca gggtgtttca gccgtccaga acagttttct cacccattcc agcacaccgg
     9061 atgccagcca ttgcatacct gggttataca accctgcctg gctatccagt tcgcaggtgg
     9121 aaccggcgta ctgacttaac agcgaagtat caacctgggc gctgcgaacc attaaaattt
     9181 cccatgtacc ggaagagagc acgggttcat tttgttcagc accagcgcca aaaagggcga
     9241 actgggtatc gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg agcattgctg
     9301 cggcgctgtt ctgtagcgta ccaatctgtt cacccgcttc caccagacga gggaagagtc
     9361 ggcgtggaat accggtggct tgtaaaattt gcggactgaa atcgcgttgc tggatatcca
     9421 gcatctggct ggttccggcc atcgtgatat cagtagtgaa ttcgccggtt aaacggtggt
     9481 taatcagcga cgaaataaag agccaggcgt gcgcgcgttc cagcagttgt ggatgatttt
     9541 ctttcaacca caccaactta tataacgtat tgaaactaaa ggctccgacg ccagaaatag
     9601 cctgcaaccg ctgtgcggag attaaccgtt caatattgtc cataaccgct gctgttcgcg
     9661 gacatttcca gctaatgatc ggatagagca gattgccttg cttatctacc agagcgccat
     9721 ccacaccaaa ggtggtgacg gcgataccgc ggatgtggca ttcagtcagt tcactattga
     9781 tttgccgaca gcaatcagca aagcgttgca aaatggcgtc taaagaccac tggtgccagg
     9841 tgttgttttc catcgcgata tcgctggcat taggcgttga ggcgcgggca acaatttttc
     9901 cctgccgatt aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt accaggatca
     9961 cttcttgttt catgatcgct cctgcaatat agccggataa cattgcttat ccggctaacc
    10021 actcttgctt gttcaggccc ggcagccgga gcgaccgggc atcacatcag ggagtaatgt
    10081 attaacgctt gtacaacgga ccgtagttct ggcaagcgcg gtaatcctgg ccttcaatat
    10141 ccatgccgtg cgcagcccag gcagaaggac gatacacttt ggtctcttca acgttgtgca
    10201 tacataccgg gatacgcagc atggaggcga gagtgataaa gtctgcgcca acgtggccga
    10261 tggtcagaac cccatggtta gcgccccagt tcgccattac cgagtacaca tccgtaaacg
    10321 gccctttacc ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt
    10381 tgttgaggat gtcatgcaca tccttcggca attccacgct ccagccttcc gcgatttgca
    10441 gtaccggtcc caggcctttg atgatgttga cacgagtcat ggtgaacggg acgccgcctt
    10501 cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc gccgggcacc
    10561 attcggtagc ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag tgcggcttca
    10621 tcgtcgggtt accttcgctg tcgcgttgtt tacaggaacc gtccagcgca gcagaaccgg
    10681 agttgatcaa atggatgatg ccgtgttctg ccagtccatc cagtttatgc cccgttacac
    10741 gctcaattgc ttctggtgac cagtaggtac gcacatcggc aaatacctga gcggtgccgg
    10801 tgagctggtg acccattagc attgccacgc cgttaagact gtcgttttcg gtcgccacga
    10861 caaagggttc gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct tcggcggtgt
    10921 caccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca gcgatggcgt
    10981 tgtagccaag tgattcttcc acgcgaccaa tatcggccag tttgctgttg ccttgcatca
    11041 tgtcgcggat acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg
    11101 cattgcgttg atactgtttg ttattttcat cttcgccata gcggaagttt ttatcagccc
    11161 aggccagtgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca
    11221 gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag aagttgtgat
    11281 caacaataga accggcgata cccatcgaaa cgccgcccag cgacagatag cttttacctt
    11341 tcatgctggc gacggccaaa ccggcgcggg caaagcgcag cagtttttct tcaacatcgg
    11401 caggaatcga tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag aatgccggga
    11461 tgcctttctg gctgtgagct gccagagccg ctgccaggta aacagcgccg ggacgttcag
    11521 tgccgttaaa gccccaaatg gctttcgggc gggttggatc catgtcgatg gtttcactgc
    11581 catagcacca gcaaggcgtt accgtaatgg tgaggcctac attctggctg ctgaattttt
    11641 cttcgcaagc agcggcttca gccatacccg cgatacaggt atcggaaatg acacactcga
    11701 cggcagctcc gcaggcatgg cgcagtttct cggtcagcag tgcggccgta gctttcgcca
    11761 tattcattgt ttgttcttca agcgactcac gaacacccat gcgacgaccg tcaataaccg
    11821 ggcggatacc aattttcggt aagctgattt tgttcattac agattcctca ctttattcgg
    11881 aaaatatgtt cagttagttg ccgtttgaga acggaaacgg gcaaagataa agatgaccgc
    11941 gaagcagagt gcggggatca gttcagcagt ggggatgttg cccgccgcgt cactgacaaa
    12001 acccatgacc ggagtgacaa taccgccgcc aatgatggtc ataacgatga aggacgaacc
    12061 gtatttggtg tcctggccga gattcttaat gcccagcgag aagattgttg ggtactgaat
    12121 cgacataaag gcgctgcata aagtcagggc tattaagccc acatgactgc cagcgaaggc
    12181 tgagatcagg cacagtgcca tagcgattaa tgcgtaggcg gccaggactt tgtgtggtgc
    12241 gaagcgactg atgagccagg taccggtgaa acgaccaata aagaagcaca ccatggttcc
    12301 ggttaaatag ttagcggcaa agcctgcagt catgcctgga atttcttcta cagcgtagcg
    12361 aatcaaatag ctccagcagg ccgtttgtgc gccaacgtag cagaattgcg ctaatactgc
    12421 ccagcgccag tggcgaatac gcgccaggcg agaaagcgat gcggagaacg atccttgttt
    12481 ggcgtcactg tgattatcac tctgcaatgc cgggaatttc gtcagcatga tcagcagggc
    12541 gaccagtaac acgatagcca cgatgatcat ataaggtgtc tgtaccgata ataccaggct
    12601 gtgtttatac gcactcaatt gctctggaga cattttatcg agaacgtctt gcgattgatg
    12661 tggcacgtta gacaaaataa gactttgccc aaagacaacc gcgataattg cgccaaatga
    12721 gttaaatgtt tgcgcaagat ttaagcggaa gtgaccactg ctttccggcc ctaataccgt
    12781 aacaaaaggg tttgcggcag tttccagaca acctaatccg gctgcaataa taaataggcc
    12841 aactaaaaat aaggtgtagt tcattatttc tgcggcgggc cagaataatg cagcacccaa
    12901 ggcatataaa aataatccgg taataattcc tgctttataa ctgagttttt tcatcaatat
    12961 cccagcagga attgggataa tgaaataacc aaagtaaaag gccgattgga tcaggccagc
    13021 ctggaaattt gtcagtgtaa aagcctgctg gaattgaggt aataaaatgt cgttaaggtt
    13081 attggctacc gcccaaagaa aaaacagtga gcacagcagc gcgaatggaa taatgtaact
    13141 tctgctttgc cctgcatctt tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt
    13201 tcccatagca tcctcttagg ttcagaagct taagcacgta atcggttatg ttttcaaaag
    13261 tgcaaaacta gcgagttcgg gcttcatatc agtgaatatt tgcctgtctg aaatgaactc
    13321 atggattcat tttcaaataa aaaacgctct gtaatatgac ggcggtcaca cttattag
//
LOCUS       DAZFKY010000131        13357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFKY010000131 DAZFKY010000000
VERSION     DAZFKY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            111..1169
                     /locus_tag="ACHKCL_003374"
     CDS             111..1169
                     /locus_tag="ACHKCL_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO5764272.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1179..2321
                     /locus_tag="ACHKCL_003375"
     CDS             1179..2321
                     /locus_tag="ACHKCL_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO5764273.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2314..3087
                     /locus_tag="ACHKCL_003376"
     CDS             2314..3087
                     /locus_tag="ACHKCL_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764274.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3089..4168
                     /locus_tag="ACHKCL_003377"
     CDS             3089..4168
                     /locus_tag="ACHKCL_003377"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO5764275.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4168..5124
                     /locus_tag="ACHKCL_003378"
     CDS             4168..5124
                     /locus_tag="ACHKCL_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO5764276.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5135..6343
                     /locus_tag="ACHKCL_003379"
     CDS             5135..6343
                     /locus_tag="ACHKCL_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO5764277.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6361..6828
                     /gene="terW"
                     /locus_tag="ACHKCL_003380"
     CDS             6361..6828
                     /gene="terW"
                     /locus_tag="ACHKCL_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO5764278.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7089..7418
                     /locus_tag="ACHKCL_003381"
     CDS             7089..7418
                     /locus_tag="ACHKCL_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5764279.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7405..7746
                     /gene="tnpB"
                     /locus_tag="ACHKCL_003382"
     CDS             7405..7746
                     /gene="tnpB"
                     /locus_tag="ACHKCL_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5764280.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7828..8658
                     /locus_tag="ACHKCL_003383"
                     /pseudo
     CDS             7828..8658
                     /locus_tag="ACHKCL_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8689..10302)
                     /locus_tag="ACHKCL_003384"
     CDS             complement(8689..10302)
                     /locus_tag="ACHKCL_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO5764281.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10333..10683)
                     /gene="tnpB"
                     /locus_tag="ACHKCL_003385"
     CDS             complement(10333..10683)
                     /gene="tnpB"
                     /locus_tag="ACHKCL_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5764282.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10680..11105)
                     /locus_tag="ACHKCL_003386"
     CDS             complement(10680..11105)
                     /locus_tag="ACHKCL_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5764283.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11159..11910
                     /locus_tag="ACHKCL_003387"
                     /pseudo
     CDS             11159..11910
                     /locus_tag="ACHKCL_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3202 a         3522 c         3492 g         3141 t
ORIGIN      
        1 tccataacca caggagttat taatcatatt atgattgaca tgtagcttag acactcatag
       61 actcacttct caatcataaa tttgcatggc gttccggata attaaaaaaa atgaacaaga
      121 aaatttggtt tatggaaggt ttatcctccc agcgagatat tattcagggg gtaaaatcat
      181 ttgcacaaaa aaataatttt gccattaccg tttttgcctc ccaccgtaac gaaagaaatg
      241 aaatcctttc cgttgccgat tattctttga ctgaacctga agatcctcaa aaacgtcttc
      301 agtttatcca ggaaaccatt cagacctacg gcatccacca tattcatact ggccgtaaca
      361 gccagtggtt tgaagaacac cgttcagcca ttgaaccgac cggtgccacc cttactaccg
      421 gtgcaacggg cgtcgactgg ttaactctgg ctgacgaaaa agttactttt gctcagttta
      481 tggagcaaaa gggtctcccg gtcgtaccat cctggcgggt gaatacgctg gcagaattaa
      541 agacacacct cgcggccccg ccgttcactg acagcccggt atgcgtgaag ccggtgacgg
      601 gtatctatgg catgggattc tggcgctttg atgacagtgc ttcgcctatg gccgtcttta
      661 atcatcccga acatcgtctg gtcagtccgc aacagtatat tgcagcagca tcagctgctg
      721 agtcgtttaa accccttgtt ttgatgccgt acctgccagg cccggaattt tccgtcgata
      781 tcctcgcgga taagggcgaa atactcgcag ccgtgggacg ccgtaaggaa ggggctatcc
      841 agtatctggt aaacgaagga agcgcctggg aactggcgtg tgactgcgcc cgtgttatga
      901 aggccgacgg gctggtgaat gttcagacgc gaaacgatgt gaatggcaac ccggtgctgc
      961 ttgaaaccaa catgcgtccg tcaggggggg tgggttatac ccttcacagc ggtgtgaacc
     1021 ttcctgggtt atttgctgcc tttaagctcg gtctgatgtc tgaagatatg gtacgccaga
     1081 gcgctaaaaa caccttttct ccggttgcgg tgagatccat tacggatgta attgcatacc
     1141 cggaatcact ctctaacctt ctgaattaag gcgtatttat gtctgatatc ctcgaagata
     1201 tgatttatcg ccgtaccctc tcatgcggta cgattcaggt aacccgtgac cagggtgaag
     1261 tttcgctcga tgacctgttt gacatcgctg aaagacgtaa cccgaaacgc gcctttctgt
     1321 ttgtcagcaa agtactcggc aggcacattc ccgtgccacc ctcagtcatg cggcaggcct
     1381 acagacagct tgccagccag ttcccctcga cactgacagg acccgtactg tttatcggta
     1441 tggctgaaac cgccgttggg cttggggccg gtgtatttga tgaagtgcgc caccagcatc
     1501 gtgaatctgt ctatctgacc tctacccgtc acccggttga tggcacgttg ctttgcgagt
     1561 tcaaggaaga acacagccac gccaccgatc atctgatcta tctgccagat gatgaagaga
     1621 aaagacgtcg tgttaccaac gcacgaacgc tggttttgat tgatgacgag gcaaccaccg
     1681 gtaatacctt tattaacctg ctttctgccc tgcgtaatac cggcaagctt caacatattg
     1741 aacaggttat cgccgtaacg ctgaccgact ggagtggcaa tgccctgtcg gagcgcaccc
     1801 ccttgcccgt cacttcggtt tcgcttgtaa gcggtcagtg gggatggacc ccattacctg
     1861 atgcacctgt cccggacatg ccgaaagtca acgtaacttc acgaggagaa tgggacatcc
     1921 aggggaaaca gtcatggggc cgactgggga tgctcgcacc tgcggccgat ctcggccatg
     1981 aggtctccgt ccacaagggg gaacgtattc tggttctcgg gaccggggaa ttcgtctggg
     2041 agccgttcct gcttgctgaa cggctcgaag ctgccggagc acaggcattt tatggatcga
     2101 ccacccgctc ccctgtcgcc gttggttatg ccattgagtc cgccatttcc tttacggata
     2161 actacgggct gggcatcccc aattttgtct ataacgtcgc ccaccagcag tttgaccgca
     2221 ttcttgtgtg tactgagaca cccgcagaaa gtattgacac gcagcttctt aaagcgctgg
     2281 ctgaggttgc gcccgtcgtg gagattgtta cctatgaata aacccgttgt tctgagcgac
     2341 cttgacgaca cgctctttca gacccgtcgt aaaatggtgg acgagctggc cctggagcca
     2401 ttccgtaccg gtgccgttga tcgcaccctg aatccacgaa gctttatgac ggaagaacag
     2461 tccatgttgg tggactggct cctggagcag gctgaactca tacccgttac cgcacgggga
     2521 actgaagaaa tcagccgcgt acggatccct ttccactcct gggcaatcac aacgcacggg
     2581 gccgtcattc tcacgcctga aggcaaaccc gacgaggagt ggaaggccca tatgctcggc
     2641 cagctggctc cctaccagga aaagctgaca tcgatgcagc gtctgatcac tgaaatgatg
     2701 gacgcaaaag gaatcaatgc ctgggcaagg ctgaacttcg aatacggtga aacggcggtt
     2761 tatatggtga tgaaacaccg cgacagcacc cgccttgacg agctcaatgc cattgcagat
     2821 gagatagaaa cggtgtttcc gaccgagggc ttctacatcc accgcaacag taataacgtg
     2881 gcctggcttc ccaccccggt tgagaaaggg ctggcagtca gctggctgct tgaaaaactt
     2941 cgggctgaac gaggagtttt ccccgtaatt ggtttgggcg acagcctgag cgatcatcgt
     3001 tttatgaaac tgtgcagctg gtttggcatc ccgcgtcaga gtcagtttgc agatgccatt
     3061 tcacagcgaa tttttggaga aaattaaaat gcagccacat gaaacattta ccggctcata
     3121 ccagcccggt gacgtggaat ttctgctaaa gccggttgtc attgagatga cgccggttga
     3181 gaaaaaagaa gaactgattc agtccgggaa gaaacattat tcggacatgc tcagccagga
     3241 gccagcgcct acacaatggc atcttgattt gtttcaccgg gcgctggatc ggggcgcaga
     3301 gaggcttgca aaagaagtca cacagctcgc tattgctctg gccgaacgct tcggtgatga
     3361 gcccattgta ctggccagtc ttgtcagggc tggcgttccg ctcggcgtta tgctgcacca
     3421 ggccctgcgt gacatgggga aaacctcatg gcattacggt atcagcatta tccgggatcg
     3481 tggaattgac ggtgcagccc tcgacgtcat tgaagagcgg catggtacca gcggtattgt
     3541 ctttgttgac ggatggacag gtaaaggcgc aattaccgga gagcttgtac gcgcactgaa
     3601 agatcgcccg ggctatcctg agcagccgcg actggttgtc ctggccgatc cctgtggctg
     3661 ctcctggctt gcagccagtg atgatgactg gctcattcct tttggcatca tgggggcgcc
     3721 ggtatcaggc ctgatctccc ggtcagtatg gtcctctgaa ggattacacg ggtgcatggt
     3781 ttgcgagcat ctcagtgaat tcgaatgcag ccggatgctt gccgataccg tcgctcattt
     3841 ccgtaagaag ttaacaccgt catccctcgc tcccctgagc tggaatacgg aatcagcccg
     3901 ggtcttatgg cagacaagtc gcgacgttat cgcgttcctg gccgatgaat ttaaagtgga
     3961 cagcgtcaat cgtattaaac ccggtattgc tgaagcaacc cgggctgtat tacgtcgggt
     4021 accggaccat gtatttgtgc gttctattga cgacccggac gttgccttgc ttgtagggct
     4081 tgctcgtgaa aagggaatag ttgttacaga aatgggggga accctcggcc agtatcgggc
     4141 tgtaaccatt atcaagaagg tactctgatg aaaaatcgtc tttctccctg gaatctggga
     4201 gccacgctat acatgcctgc aacacgggaa gatattgctg atgccgtcct gcacgggaaa
     4261 atcccgggct tgcgctctct ggtgatttgc ctggaggatg ctgtcagtga agcagacatc
     4321 cccgtcgccc tcaaaaatct ggagcacctg ctgcacgagc tcagtaacag catgagtagc
     4381 ctgggtaaaa atgactggcc tctggtgttc attcgccccc ggcatgccga aatgggcaga
     4441 tggttaaaag cgcattatga tctttccgct gtcgacgggt ttgtgctgcc taaatttacc
     4501 ctcagttcgt tagctgaatg gtgggatatc atggtcggga ctcacctgtg catgatgcca
     4561 acgctggaaa cagaagatgt ctttgacgtg gttcagatgc gcgagctggc tacccgtctg
     4621 gaggaacatc cctgccacga ccgcattatt gcgcttcgaa tcggcggcaa cgatcttatg
     4681 aacgttgtgt cgcttcgccg tccccgggac ctgacgctgt atgacagccc gatgggctac
     4741 gtcatcaaaa tgctcgtttc ggtcttcggc ccgcgtgatt ttgccctgac cgcgcctgta
     4801 tgtgagcata ttgacgatca tgccgttatg gccagagagc tggctcttga tatggcacac
     4861 gggcttgttg ggaaaacggc cattcacccg ggtcagatag aggtcattca aaacgcgctg
     4921 atggtcactc agggtgagca ttctgacgct ctgcggatcc tgaactctac ccaggccgtg
     4981 tttaagtcgc agggagcaat gtgtgaaccc gccacacatc gccgctgggc ggctggcatt
     5041 ctggacaggg ctcgttttta tgggttacag aacgagcaaa gcgctgatgg aatcagatta
     5101 cttaccgtga cccagcatca ttaaggaaat cataatgggg ttcaggttca gaaagtcgat
     5161 caacattatt cctggcgttc gcctcaacct gagtaacggt gcaccgagcc tgagtgtcgg
     5221 gccgagaggt gcttccgttt cttttggtag ccgggggacc tatgccaatc tgggcttgcc
     5281 cggtaccggg ctgagttacc gtacccggct tgaccgggcc gcgcgttcca gaggtgaaaa
     5341 ccggacggca accgacccgg ggctcagaca ggcgcttgag cagaaagccg ctgaactcat
     5401 gtcagcggta accgcaatcc gtaatattca cgagctgacg ccggatccaa aaacaggcat
     5461 cagctgggca gagctggaag cagtatacct gcataacaga acgtcgcctt ttcaggttcc
     5521 ggcaccggtg cgtccagaaa agccagacta ccttgtattg ccggaaaagc ctgccgagag
     5581 cgaaggcatt agttttttgg gtaaatggtt tgaatcggaa tcagctaaag ctgagcgcca
     5641 cgccgaaaat cttcgccggt ggcagcagga gctgattgat gtggagcgtg agaataccct
     5701 tcgacagcac cggtaccagc aacaacggac ggcctgggcc gaacagtatg caaactggaa
     5761 gtttgaagct gaagaacatg aaaaacggct cgccacggct caggcagatg cccggcagca
     5821 gttccggaca gacgccgcgt ttttcgaatc atacctggcg ggtgtgctgg cagaaactga
     5881 atggccgcgt gaaacgcttg ttgcatttga agtaaagccg gagctatcag cagtcctgct
     5941 ggacgttgat ttagctgaga ttgaagattt ccctgataag atttacggcg ttaatgcccg
     6001 gggaacggag ctgacggaaa aagccatgac gcaaaaagcc gtacgcgaaa actatgccca
     6061 ccacgtccat ggctgcttgt tccgcctggt cggtatcgtt ttacatacgc tacctttcga
     6121 caacgtgatt gtgtcaggct ttacgcaacg ggtcagtaag cggaccggct atctggagga
     6181 tgagtatatc ctgtcctgca aatgcactcg cagccagatg tcgtcagtaa attttgcagg
     6241 cataaaacac attgatccgg ttgaagcgtt aggcgatcaa ccggttattc gaaagatgag
     6301 cagtaccttc atttttcagc ctattgaacc actaaccctt taagcaggct aaatcttacg
     6361 atgcaattaa ataccagaca ggcccggatt tttaagcttg ccaacttact gggcaccggc
     6421 aagcccgttt ccgctgccga cattataacc agtctggaat gctctgagcc gacattaacc
     6481 cgggccctca aagagctacg cgagtcatat tctgcggaga tcaaatacag caaggcaggt
     6541 cattcctatc atctcgttaa tccgggccag ttggataaaa agaccctccg ccggatgaat
     6601 gaagcgctcg cacagaatgc tgaactgaaa accggtgagt ccacagggaa ggtcgtactc
     6661 gataaggata aaaaaacagc cgtgtcatta tcgctacgca tgcggattct aagaaaaatt
     6721 gaccgtctgg ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa gctggctctg
     6781 cactccgttg atgaactgat aaaagaatac agcgctaaaa agtcctgacg ctaaatctca
     6841 gaccggggtg aaaactccgg ttttgtcata tttcacctct gtattggcgt gatgcaaaat
     6901 ggcatctgaa tcgatcatgg tttggcatcc atttccgatc actgtttggc atcacaatcg
     6961 atcacgattt ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta cggggaagtc
     7021 atcttcacag ggatcttcag tcagttagca ttaccgacat ccattcacca acttaccgga
     7081 gataatcaat gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac gttgccgcat
     7141 ggcgcgccag cggccttacc cgtgaacagt actgtgaact ttacgacatt ccgtttaaat
     7201 ccctgcgcca gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga gcaccagaaa
     7261 ttattcctgt ttcagtatct ggctcttccg gaatgactga tggcagacca ttgtcggatg
     7321 agcctgtcac tctcttcctg cccggtggta tccggatgtg ctgccagcct tctcagttaa
     7381 ccgatgtttt ccgggctctt cgccatgctg acgcctgata acgtattcct ggtcgtaaaa
     7441 cccgtcgata tgcgccgggg tatcgacaca ctgacacagt acgtccagaa cgaacttaac
     7501 gctgcatggc atgacggtgc tgcttttgtc ttcaccaata aagtccgctc ccgcataaaa
     7561 gttctccggt gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca ccggggaagc
     7621 ttccgctggc cccggaaagg tgatgccaca tggcatctca cacaggatga gtttcactgg
     7681 ctggtctttg gggttgactg gcaacaggtg aaagggcatg acctggcaaa atgggtatac
     7741 cagtaagaac ccgaagatgc tcagagcaac gcaataactc aatgaaatat aatgaatatc
     7801 catgcgtgat gtcatataat ccaccgcatg gatatctcgc ttctcagtac caccagtgac
     7861 cctgaacagc ttcgcgctct ggcaattgcc atggtgcaaa aagtcatggc tgagaatgca
     7921 gaacttcaga accgtatccg catcctggaa gagcagatga agctcgcacg ccagcagcgt
     7981 ttcggtaaaa aatgcgaatc gctggccggg atgcagcgtt ccctgttcga agaggatgtt
     8041 gacgcggata tcgcagaaat ctcggcgcat ctggacaaac tgcttcctca gaccggggac
     8101 gaagaaaaaa caacaacacg tcctgttcgt aagcccttgc cttcacctct tccccgtgct
     8161 gaaaaggtta tcccgccagc tgaggaacgt tgtcctgact gtgatgcccc cctgcacttc
     8221 attcgcgatg aagtcagcga gaagctggaa tatattccgg cacaggtcgt ggtcaaccgc
     8281 tatatccgcc cgcagtacag ctgtccctgc tgtgagaaag tgttcagcgg aaaaatgccg
     8341 gcacatatcc ttccgaaaag tgctgttgag ccatcggtca tcgcacaggt tgtcatcagc
     8401 aaatataccg accatctgcc cctgtatcgc cagcagcaca ttttctcccg aatgggggtg
     8461 gagctccccg tcagtacaat ggcggatatg gtcggtgttg ctggcgctgc actggccccg
     8521 ctggcaaaat tgttacgcca cgagcttctg acacgggatg ttattcatgc agatgaaacg
     8581 tccctgaggc tccttgatac ccggaaaggc ggtaaatcct gctccggctg gctgtgggct
     8641 tacgtcagcg gagaaagagt aagcgtcaac ggagcaccgt attgacgctt atttattggt
     8701 gagtactacg ttccatggca ggagttcatc aactcggttg gaaggccatt ctggcagtac
     8761 gctcaggata tggcgcagat acgcttccgg atcgataccg ttcagacggc aggtgccgat
     8821 cagcccgtac aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga acataaagtt
     8881 tttctttccg agacagactg cacgaagcgc tctttccgca gcattattat ccgcctccgc
     8941 cagaccgtca tcactgtaat aacagagggc atcccactga ttcagtacat agctgcacgc
     9001 ttcgcccagt ctggattttt tcgacagcgt accattcttc tccaccatcc attcatgcag
     9061 tgacgtcagt aacactttgc ttcgctgctg cctgacggca agacgctctg actccggtaa
     9121 tccccgtatt tcatcctcga tggcgtacag ttcactgatt cgcttcaggg cttcttctgc
     9181 cgtcgcactt ttgctgctga tgtatacatc gtggattttt cgccgggcat gggcccagca
     9241 cgcaacttct gtcagtgcac caccttcacg ttctgcactg aacagccgat cgtaaccgct
     9301 gaacgcatcc gcctgcagga taccccggaa gggacgaagg tgttgtaccg gatgttttcc
     9361 ctgcctgtct ggtgagtagg cgaaccagac cgccggtggc tctgatgagc ccgcattccg
     9421 gtcatcccgg acatacgtcc agatgcgtcc tgtttttgcc ttttttctgc ccggtgccag
     9481 cacttttact ggtgtgtcgt cagtgtgaac cttgcgggtg ttcatcacgt aacggtacag
     9541 ggtatcattc agcagagtca ttaactgaca acatgcgtca acccagttgg agagtaatgc
     9601 ccggctcagt tcgatgccct ggcgggcaaa gatttcactc tgacgataca gtggcaggtg
     9661 ttcgcagtat tttcccgtta acacgcgggc aagtaatccg gagcccgcga tgccgcgctc
     9721 tatcgggcgg gacggcgctg gcgcttcaac tatacagtca cattttgtac aggctttttt
     9781 tacccgaaca gtgcggatca ctttcagggc gctactcacc agttccagct gctcagcact
     9841 gacttccccc agataatcca gctcactgcc acactccggg caacaacttt cttcaggctc
     9901 cagacggtgt atttcacggg gaagatgtgc cggtaacgga cgacgatgac gtgactgtcg
     9961 caactggcgg ggaacctgcg ggtcatcctc acgcccactg taacgatcgc tttcctgttc
    10021 gcgttgtttc agttgggcct cagcctgttc aacctcacgc tgcagttttt cagaacgggt
    10081 accgaacaac atccggcgca gtttttctat ctgggccctc agatgttcta tttcccgctc
    10141 ctcctcttcg atcttttcct cggcacgtgc cagtgcagag cgcaggaagg cctccgtctc
    10201 ttcaaccaga ctcagttgct gatctttctg acggagggct tcagcctgct cagagagcag
    10261 cctttccagc tcagtgatac gaatgaggta tttccgactc atgaccgttt ttataatccg
    10321 gccatgacat ttttacaaca ttgtcagtgc attaaggcgg gatgttttgg gctgacgcca
    10381 gtccagttta tcgaggagca ttgccagctg cgagcgggta atggatacct taccgtcacg
    10441 caccgcaggc cagataaact ggccttcctc cagacgtttg gtgaacaggc acagaccatc
    10501 agcatcagcc cacagaattt taatcgtgtc accccgtcgg ccgcgaaaga taaacaggtg
    10561 accggagaag gggttctcat ccagcacatg ttgtacctgt tctcccagac cgttgaagga
    10621 tttacgcata tcagtaacgc cggcaaccag ccagattcga gtgcctgatg ggagcgagat
    10681 catcgtcccc tcccggtcag ttcacggatc aacaccgtga gcagctctgg tgaaggattt
    10741 tccagcgtca tgttaccgtg gcggaactca actttacagg aactggcact gactgtgctt
    10801 tgtgaaggag tggataaaag cggagtaaga gccgccatag gctctttctg ctcatcaggc
    10861 gttatctcaa caggtaataa ttcaacgcca gcgccagaag aggttgttac cggaagacgc
    10921 cgcgatatac gcctttcgtt ctgccagagc ctgagccatt tgaacaggag gttatcattg
    10981 atatcgtgtt cccgggcaat acgggcaaca gaggctcctg gttgtgaagc cagtttaacc
    11041 atttgaagtt taaactcatt tgaaaatgat ctgcggggtt ctgaggataa tattttctgt
    11101 tccataacag gtgtccacta gttgaaaagg tgggcaccta cgttaccaat actggcttaa
    11161 tggctacata cggcggtcag tttacgctta cgagaaagaa gcggtccgcc ggtcgtctgt
    11221 tttgactcgc agaccggacg ggccctcaga tatcctgagg cctggctgca gggctggtgt
    11281 ggcggtacgc tggtgagtga cggatacagc gtgtacaaat ctctggcgga taatgacccc
    11341 ggtattacat ctgcctgttg ctggagccac gcaaggcgcg gtttcgcaaa tctgtataag
    11401 gccagcaggg agccccgtgc agccatggcg ctgagaaaaa tcgcgggtct ttatcgcatt
    11461 gagaagttca tccgtgaacg tcctgtggaa aaaatacggc aatggcgaca acggtattcc
    11521 cggccgatag tgaatgacct gtttgcctgg cctgaagagc aggagccgtg ctgtccgccc
    11581 gacggaccac taaacaaagc gattaattat atcctgaacc ggcgcgatga actgagctgc
    11641 ttcctgagtg acggagccgt gcccctggac aacaatatct gcgagcgtgc tatccgcccg
    11701 gtggtcatgg ggcggaaagc atggttattc gccggttcac tgatggccgg gaaccgcgcg
    11761 gcacagataa tgagccttct ggaaacggca aaacgtaatg gtctggagcc gcatgcctgg
    11821 ctgacagacg ttctgacgcg tctaccggag tggccggaag accgactgga agagttgctg
    11881 ccccttgagg gatttacctt ctccgggtga gtgatacctg ccatcaggtg ttcgtgcagc
    11941 gggccataac ccgcagtcgg gagttgaacg cctgacgaca ggaaatgagc cagagcagcg
    12001 accacgcggg ctgacagtga gtcagaacct gaatcagccc ggagccattc ccggggctgt
    12061 acttcatcac ctgccatcag tacccgtgtc agtacttcgc gacctgacgc aattacccga
    12121 ctcagttctt cagaacccgc ctccgatacc agcctggcgg tcgcccctgt gcaacaaaat
    12181 aaatcaacca tcgtcagacg gtgatcaatc gtatgcgctg ccagtggctc gcctgaaaaa
    12241 tgcgtcaata tgagttcgcc ggaacaatac ctcagttctt cagaacccgc ctccgatacc
    12301 agcctggcgg tcgccccggc gcaacagaat aaatcaacca tcgtcagact gcgatcattc
    12361 gtatgcatcg ctgatgatgc gcccgaaaaa cgcgtcaata tgagttcgcc ggaatattac
    12421 tgacgacacg gtttgtagtt acgttccttc ggtgagtgag tacctgtgcg tcagggtaac
    12481 gcaatcagtt caggtttagc ggggtgtcaa gtcggtttgg ctaaccgacc gcagaattaa
    12541 ctgtaccttt aaaggtcgcc cccgatatat ctgcctgtca gacatatcgc cgattcggta
    12601 tctcactaca taaaactcct taaaaaacaa tgcgattctg ttggtacgaa gctttcagta
    12661 agtcagtccg gagaagcgcc agtacgatac agaatgaggt tgaaaaaaac gttcacgtct
    12721 ggtcacttct tcaaatgtta aaaaacatct cgtaaagagc cagtttatgc gggttcgttg
    12781 agtagacgtg cgagcgtcac tttgtcagct ttaacgctga cgaaagtgac gcccgcacag
    12841 cttcaaactc cacatgcaca cgcaagcgac caaatttcaa atggtcttat gccgtgcgca
    12901 cagtcataag aagcgcatgc ggaaagtcag aaaatatctt aagacgcaga ttttaaaggc
    12961 gtttttatgc cagtctgccg ggaatacaca gtagtccggt tgtccggaag caccacggta
    13021 atccgaagga aaaatcaacg tacctcaatc accgtagtga tatccgttcc cagaggctaa
    13081 acggatgtac caccataaag gcatcctctt tcaggctacg aggatattgc aatcacccga
    13141 aggcgtttct ctcagattgc tgaaacattg gctggcagtc acccgaaggc gtccctctca
    13201 gactgctgga acattggctg gtcgcaaaag cgacagttaa tgcgaataca ggttaacaaa
    13261 aagggaaatc tgccgtcaac ccaaccatat gaacatgttg atttcattat gaatgtgatg
    13321 ttctaatata aattaatcag taacttcata aaaagaa
//
LOCUS       DAZFKY010000132        13343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFKY010000132 DAZFKY010000000
VERSION     DAZFKY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..166)
                     /locus_tag="ACHKCL_003388"
     CDS             complement(<1..166)
                     /locus_tag="ACHKCL_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764284.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQ"
     gene            426..1346
                     /gene="cbpA"
                     /locus_tag="ACHKCL_003389"
     CDS             426..1346
                     /gene="cbpA"
                     /locus_tag="ACHKCL_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5764285.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1346..1651
                     /gene="cbpM"
                     /locus_tag="ACHKCL_003390"
     CDS             1346..1651
                     /gene="cbpM"
                     /locus_tag="ACHKCL_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5764286.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1805..2404)
                     /gene="torD"
                     /locus_tag="ACHKCL_003391"
     CDS             complement(1805..2404)
                     /gene="torD"
                     /locus_tag="ACHKCL_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5764287.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2401..4947)
                     /gene="torA"
                     /locus_tag="ACHKCL_003392"
     CDS             complement(2401..4947)
                     /gene="torA"
                     /locus_tag="ACHKCL_003392"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5764288.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4947..6119)
                     /gene="torC"
                     /locus_tag="ACHKCL_003393"
     CDS             complement(4947..6119)
                     /gene="torC"
                     /locus_tag="ACHKCL_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5764289.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6249..6941
                     /gene="torR"
                     /locus_tag="ACHKCL_003394"
     CDS             6249..6941
                     /gene="torR"
                     /locus_tag="ACHKCL_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5764290.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6914..7942)
                     /gene="torT"
                     /locus_tag="ACHKCL_003395"
     CDS             complement(6914..7942)
                     /gene="torT"
                     /locus_tag="ACHKCL_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5764291.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            8025..10769
                     /gene="torS"
                     /locus_tag="ACHKCL_003396"
     CDS             8025..10769
                     /gene="torS"
                     /locus_tag="ACHKCL_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO5764292.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10841..11914
                     /gene="yccM"
                     /locus_tag="ACHKCL_003397"
     CDS             10841..11914
                     /gene="yccM"
                     /locus_tag="ACHKCL_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5764293.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11963..12097)
                     /locus_tag="ACHKCL_003398"
     CDS             complement(11963..12097)
                     /locus_tag="ACHKCL_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5764294.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12125..12355)
                     /gene="ymcE"
                     /locus_tag="ACHKCL_003399"
     CDS             complement(12125..12355)
                     /gene="ymcE"
                     /locus_tag="ACHKCL_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5764295.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12330..12518)
                     /gene="ymcF"
                     /locus_tag="ACHKCL_003400"
     CDS             complement(12330..12518)
                     /gene="ymcF"
                     /locus_tag="ACHKCL_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5764296.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12529..12741)
                     /gene="cspG"
                     /locus_tag="ACHKCL_003401"
     CDS             complement(12529..12741)
                     /gene="cspG"
                     /locus_tag="ACHKCL_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5764297.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            13027..13239
                     /gene="cspH"
                     /locus_tag="ACHKCL_003402"
     CDS             13027..13239
                     /gene="cspH"
                     /locus_tag="ACHKCL_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5764298.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         3225 a         3487 c         3369 g         3262 t
ORIGIN      
        1 cttgttgacg tctgatttct ttatacaacc gcataaaaca caccagtgtg ttttcaaaaa
       61 gcgtgattga atatgtttta tttttttcgt actcattttt tatatcgttc caggcttgct
      121 tcaaataatt atttgcagtg caactaattc catgtatatt acccatatat agcgcctata
      181 aaatttaata aataataacg cactagtaaa acttaaagtg cctgtttcaa ctatcaaaaa
      241 tcgctcaccc tttttcacct gtttaaaata tgttcagcgc cccaccttga ggcgacctcc
      301 tctccgcgat gatttcaata acatattctg tgttggcata tgaaattttg aggattaccc
      361 tacacttata ggagttacct tacaggggtt ccttcaattt gtgttgattt acgcgagata
      421 acgctatgga attaaaggat tattacgcca tcatgggcgt gaaaccgacg gacgatctca
      481 agacaatcaa gaccgcctat cgtcgacttg cccgcaaata ccatcctgat gtcagcaaag
      541 aaccggatgc cgaagcccgc ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg
      601 aacaacgtcg cgctgagtat gatcagatgt ggcaacatcg caacgatccg caatttagcc
      661 gtcagttcca gcatggcgac ggtcagagtt ttaacgccga agattttgac gatatcttct
      721 cgtcaatttt cggtcagcat gcccgccaga gccgtcaacg ccccgccgca cgcggccacg
      781 atattgaaat cgaagtggcg gtattcctcg aagaaacgct tactgagcat aagcgtacca
      841 tcagctataa cctgccggtt tataacgcct ttggcatgat cgaacaggaa atcccgaaaa
      901 cgctgaatgt gaagatcccg gcaggcgtcg gcaatggtca acgcatccgc ctgaaaggcc
      961 aggggacgcc gggcgaaaac ggcggtccaa atggcgattt gtggctggtg attcatattg
     1021 cgccacatcc gctgtttgat attgtcggcc aggatctgga aattgtggtg ccggttagcc
     1081 cgtgggaagc ggcgctgggt gctaaagtca ccgttccaac actgaaagaa agcattttgc
     1141 tgactatccc gcctggcagc caggccgggc aacgattgcg cgttaaaggc aaaggtctgg
     1201 tgagcaaaaa acagaccggc gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac
     1261 cggatgaaaa cactgccgcg ctgtggcagc aactggcaga cgcccagtcg tcttttgatc
     1321 cacgtaaaga ttgggggaaa gcataatggc taatgttacg gtgactttta ctattaccga
     1381 attttgcctg cataccggca tctctgaaga ggaattgaat gaaattgtcg gtttgggggt
     1441 ggttgaaccg cgtgagatcc aggaaacaac ctgggtattt gacgaccatg ccgccatcgt
     1501 ggtgcaacgc gcggtgcgcc tgcgtcatga actggctctg gactggccgg ggatcgcggt
     1561 ggcgctgacg ttaatggatg atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca
     1621 gcggctttcc cggtttgtag ctcatccgtg atgtgtgttt gccggatgtg atgacgcatc
     1681 ttagcctgcc tgcaaagagt acttttcctg tgggccggat aaggcgtgtt cacgccgcat
     1741 ccggcggcgg tgccctaatg cctgatgcga cgctaaacgc gtcttatcag gctaacaaac
     1801 gacgttatct gttttggtgg tcgccctcaa ctaacaccag caataactgg cttagtgccg
     1861 cgtaaaaacc aaagctgtca tactgatggc aacgcgcaac aaactctggt aaccattgcc
     1921 gcaacgccgt gagtgttttt tgccgtaaac tgtcgattct tcgcgcagga accgtcccct
     1981 ctcccagcga aaaatgcaga tgactcaaca actcgagata gatcgccaga tgatctgtcg
     2041 gttcgttgaa attgccgctg gtttccatcc ctgcctcaac taacaagcgt ttaatctctt
     2101 gctcgtcctg tttgtaggcc gatgcatacg gcagcgccgc ttgtttgtca gtcatcagaa
     2161 acaggccgca aaagtcagcg gccagttcca gacgggcatc gtcacgtact gtcagagtgg
     2221 caacacggtt ttccagctca tccaccgccg cagtgagcgg cggttcgctt ttcagcaacg
     2281 aaaaccattc agccatctgc gcactggcga tttgcgtcag ttgttcatcg tccagttcac
     2341 gggagaacaa ctgcgccagc caggcgtaaa cacaggctat ctgttgtgct gtcagcgtgg
     2401 tcataatttc acctgcgacg cgggaacata ttcgcactgc gccaccatct ccacggggcc
     2461 gttaaacgcc gttacctgct ccactgttcc gttgcacttc tcaatttcca ccagcgtagt
     2521 gtgcgcactg gtcgcctgcg ccagctgcga agtaccgatg tcaatggtca acacgttggg
     2581 gttaccgtat ttgcacagcg caccaggctc gccgccttta tctggatcgt gccatgcccc
     2641 ttcgtgaatt cgcgccacgc cgggtgcata gcggtcagaa actactgccc ctgccaacac
     2701 ctgaccgcga gcgttaaaga cgcgtaccac atcaccgtta cgaataccgc gcgcgctggc
     2761 atcctgcggg ttaatgaaca ctggctcttt acccgctacc gtatattgct gacgcagagt
     2821 ttccgactca cataactgcg agtgaagtcg gaaatccgga tgcacagatt gcagatgcaa
     2881 cggatacgtc tgcgagccgg gcccaccgtg ggagcgttcg attttctcaa accacatcgg
     2941 atgcccctga caatcgtcgt agttcatatc ggcgatggtt ttcgaataga tctcaatcag
     3001 gccactcggc gtgcccagcg gttcgagatc tggatcttcg cggaatgcct ggtggcgaac
     3061 aaacatctgc ggatggtcaa actcgacgta ctctttgtta ttccagaagt catcaaacgc
     3121 tggcaaatga acgccgcgtc ctttgccttg ctgaacacct tcctgccaga tgcgtttcag
     3181 ccagcccatt tcgtccagcc cttcggtaaa ggcttcttcg cgattaaagc ggcggcacag
     3241 ctcgcggaaa atatcaaagt cgttgcgcgc ctcgaactgc ggcggcacga cctgtttcat
     3301 ggcgataatg ccacggttgg agtggttgcc gtactggtcg agatcgttac gctcaaactg
     3361 cgtggtcgca ggcagcacga tatcggcaaa gcggcaggtt gaggtccact ggttatctat
     3421 ggcgataacc gtttccagtt tgcgccagcc ttcaataatg cggttgatct gctgatggcg
     3481 atggaagggg ttagttccgg caaaaataca cattttcagc ggcggcagtt ttaccgattt
     3541 accgttccag ttgattactt tccccggttc gaggatcgca tcgataaaac gggcaatcgg
     3601 aatggtgctg ctgtagcctt tgtaatcact gttgtcgtga acaggcggaa tcgacgtaga
     3661 gccggagaaa ccactcagaa taacgccttt acgccccggc gtgcctgcgc cgttatagtg
     3721 ccagccaaaa ccaaagccac cacctggcag gccaatttgc cccagcatcg ccgccagaac
     3781 cacaatcatc cacgcccact gttcaccgtg ctgcatacgc tgtacgcacc agccagcaat
     3841 aatctgcgtt ctgttcgccg ccatctgccg cgccagtcca cgaatggttt cggcatcaat
     3901 gccggtcagt ttttcagccc atgcggcatc tttcggctga ccgtctttct cacccagcag
     3961 atacggcagg aactgctcaa aacccacaca gtagttagcg aggaagtttt tgtcgtacag
     4021 gttttcactg tacaacgtat gcgccagcgc cagttgcagc ggcacgtcag tttgcgggtt
     4081 aaccgcaatg tgcttcacat gctcgcgccc cagatactca tgggtggatg taacaaccgg
     4141 atcgatgcta atgacctcaa tttcaccggc ggcgactttc gctttcaact gcgcgtaata
     4201 ttcataaaca tcgtgatccg ggcaccacca gttcgcttgc tggtttttca gcaaatcgga
     4261 gccccacagc acaatggttt tgctgttctg caataccagc ggccaggagg tttgctgttc
     4321 atacacttcc atcgaaccga ctacgcgcgg caggatcacc tgcgcagcac cagtagagta
     4381 atctccgccc gtaccaacgc tattaccatg caaggcaata gctttcgcca gcatccccga
     4441 agcgttatgg aacatccccg tcgattgcca accactggcg gtcagcaagg cactcggccc
     4501 gtgagttttc tgtacgcgtt ccagttcttc atagaacatg tcgagggctt catcccagct
     4561 cacacgcaca aaacggttat caccgcgctg ggaggtgtcg ctcagatggc gcttacgcag
     4621 ccagtccacg cgtaccatcg gataacgaat acgcgccgcg ttgtgtacat gatccggcaa
     4681 tccggcaatc attttcgacg gatatttatc cagttcgaac ggttttgccg ccacaaagcg
     4741 accatccttc accgtcgcgc ggatagcccc ccagtgcgac ccggtaagaa tgccctcttt
     4801 cgagatgaca gcctcagtcg ccgcttgcgc cgcagtcgca cggcgcgacg ttaacaatga
     4861 cggccccagc atcccggcga cggttaagcc gccgagttgt gccagaaaac gccgacgtga
     4921 tgcctgaaag agatcgttat tgttcattat ttttcttcct tcttatcgcc gtgagcctta
     4981 cctgcggtgt cagacgcatt catttgcaga tatttcaaca aggtgcgttc ttcacgttta
     5041 tcgaggctgg taaagccgat catgccgttg agcgtgccaa tccagccgtt agcgtcaaag
     5101 tgagagattt ccggtgcgcc gtggcactgg ttacaggtgc cgttgtacaa cgaatccgca
     5161 taagcccaga tcggtttgat atcgttcacc atgtcacctt tcttcatcca cgcggtggcc
     5221 tgcaacttgc tccactcggt attggtgtcg gcaacggtgg ttttctccag cgtttttacc
     5281 tgctgctgca catcaccacg aatcgaggca acaaagatgc gtttacctgg gaattgggtg
     5341 agtacacgct gacgtccggc gctttctgtc cagccggtaa tttcaatttg cagccagtcg
     5401 ccgtcacgtt taaggacttt cacttccgag gcaggcagca gagaaccaga ggcttcttta
     5461 tcgcctttcg ccgcataaat tggcttaata tcaatagagt acagcgtgtc accactgtca
     5521 ttagcaccgg cgcgcagctc atcgaactgc ttacggaagc cgctactcat atccggtaac
     5581 tggtgggcaa tacctttatg acagtcgatg caggattgat tatctttcgc tgccaccttc
     5641 atctgacgtg ccgcttcagg atgctgcttc gcatgatcca tcgcatcgta gttatggcag
     5701 gagcgacagg ttgccgagtt gttttctttc attcgcgccc attcacgctc ggcaagttcc
     5761 gcgcgtttgg cttcaaattt ttcaggtgta tcaatggagt gggcaataaa ggtctggtag
     5821 atatcgttgc tcgcttccag tttgcgcttc accatgcctg gaatatccgg cgggatatga
     5881 cagtcatggc attcagctcg cacgccggag gcgttctgga aatgcaccga ctgtttatat
     5941 tcttcataca ctggttgcat actgtggcaa ctgacacaaa attcggttgt gctggtgact
     6001 ttgatcccaa cgtgtggcaa tacaatcagc gcaatgccaa tcacaatccc aatagcgacc
     6061 agcgccagta ccgaccaacg agcactgggt cggcgtagcg cgttccagag tttccgcata
     6121 atagcccctg taaaattatg gtttagtgaa gcgatcttaa tgagtaaata tgaacagcgg
     6181 cactggtcag gatgaacggc ttacggcaga atatgaacag atatgaacag aatgagtaaa
     6241 accctctgat gccacatcac attgttattg ttgaagatga gccggttacc caggcgcgat
     6301 tacaatccta cttcactcag gaggggtata ccgtttccgt tacggcgagc ggtgccgggc
     6361 tacgggaaat tatgcagaat cagccggtgg atttaattct gctggatatc aacttgcccg
     6421 atgaaaatgg cctgatgtta acccgcgccc tgcgagaacg ctcaacggtg gggattattc
     6481 tggttaccgg acgcagcgat cggattgacc gtattgttgg gctggaaatg ggcgcagacg
     6541 attacgtcac caaaccgctg gaactgcgcg aactggtagt acgggtaaaa aatctgctct
     6601 ggcgaatcga cctcgcgcga caagctcaac cgtacactca ggacaactgc tatcgctttg
     6661 cgggttattg cctgaatgtg tcgcgccata cgctggagcg ggatggtgag ccgattaaac
     6721 tgacccgcgc agagtatgaa atgttggtgg catttgtgac caatccgggc gaaattctca
     6781 gccgtgaacg tctgctgcgt atgctctctg cgcgtcgggt ggaaaaccct gacctgcgca
     6841 ccgtcgatgt gttaattcgt cgtttacgcc ataaactcag cgcggattta ctggtgacgc
     6901 aacatggcga aggttatttc ttagccgctg atgtgtgctg ataaaaatag accggacgaa
     6961 atcccccagg cgacagcgag cggcggatat gttcgcggtc ggcatttttc ggcgtcagaa
     7021 ctaaaatcgg tgggctgaca ttatcagaca ccgattgccc ctgtaattgc ctgatggcct
     7081 gctcaactgc cagttccccc tgccagacca tttgatcgct ggcagccata atcactcttc
     7141 cccgcttcag cccgcgatac acctgatgtg aaagataaaa cgacaccacg gtaagcggcg
     7201 ttttcaggtt acgcccttcc cccattgccg cctctgccgc aatggccgtt ccggcaacga
     7261 cgtcgatttc tggatggcgt tccagcatct cctgcaacag gttacgctgg atttcaatat
     7321 cgttatcacc aagcgcaata tcaacaatac gcaccgggct tccggcaatg gctgcgcgaa
     7381 aaccctcgac catctcctta ctgcccccgg cgttatcggg tccgggcatc aacagcacat
     7441 tcagtggttt accgtgcgcc cattgcacca gatatcgccc aggttgatag cccatctgaa
     7501 accagggcac accaacgcgg cttttcacct ggggagcatc aatagcattt accagctcga
     7561 tcaccggcag atttgctact tgcttttgca gatcgggaaa tgacgtcgtg ctactaccga
     7621 gcaaaatggc ctctgcgccc cactgtttac actggtcgat ttgtgcttgc tgggtagcca
     7681 actggctgta gccgcctgcc tccagcactt ttaaatccac accgtagcgg cgagcagcct
     7741 cctgcatacc atagttcaac gataaccaat atgaatcttt caggctggga taaagcgcgc
     7801 acagtttcca tacgcgtttg gcttttagcg gcatagaggc ttgcaccgtg aaatgctgcg
     7861 catcatgcca gcgcaacagg ttatcagccg aaaatgccgg caacatgaaa agggaaagaa
     7921 gtaaaaatag cagcacgcgc atgatagcct catcaataat aaggctttat gctagatgca
     7981 ttccgctttg cgactcaaca tttttcacct taagtgcacc gaccgtgaat ttaaccctga
     8041 cccgaagact ctggatgggc tttgccctga tggcgctgtt aaccctgacc agtaccctgg
     8101 tgggatggta caacctgcgc tttatcagcc aggtggaaaa agacaatact caggcattga
     8161 ttcctaccat gaatatggcg cgccagttga gcgaagccag cgcctgggaa cttttcgccg
     8221 cgcagaacct gaccagtgcc gataacgaaa agatgtggca ggcgcagggg cgaatgctca
     8281 ccgcacaaag cctgaagatt aatgcgttgc tgcaagcgtt acgggaacaa ggttttgata
     8341 ccaccgctat tgaacaacag gagcaggaga tctcccgttc gttacgtcag caaggggaac
     8401 tggtggggcg gcgtctgcaa ctgcgccagc aacaacagcg actcagtcag cagatagtcg
     8461 ctgccgccga tgagatcgca cgcctggcgc aaggtcaggc gaataatgcg gcaacctccg
     8521 ccggagcgac ccaggccggg atttacgatt tgatcgaaca agatcagcgt caggctgctg
     8581 aaagtgcact cgatcggctg attgatatcg atcttgagta tgttaaccag atgaatgaac
     8641 tgcgccttag cgctctgcgg gtgcagcaaa tggtgatgaa tctggggctg gagcagatcc
     8701 agaaaaatgc gccaacgctg gaaaagcagc tcaataatgc ggtgaaaatt ctgcaacgtc
     8761 ggcaaatacg cattgaagat ccgggagttc gtgctcaggt cgcaacaacg ttaactaccg
     8821 ttagccaata tagcgatttg ctggcgctgt tccagcagga cagtgaaatc agcaatcacc
     8881 tacaaactct cgcacaaaat aacatcgccc agttcgcgca gtttagcagc gaagtcagtc
     8941 agctggtcga caccattgag ctgcgtaatc agcacggact ggcgcatctg gaaaaagcca
     9001 gtgcacgcgg gcaatacagc ctgttattgc tggggatggt ttcactttgc gcactgattc
     9061 tgatcctctg gcgcgtggtt tatcgctcag tcacgcgtcc acttgccgaa caaacgcagg
     9121 cgctgcaacg gctgctggac ggtgatattg actccccttt cccggaaacc gctggcgtac
     9181 gggagctgga caccatcggg cggctgatgg atgcgtttcg cagcagtgtt catgcactga
     9241 atcgccaccg cgaacagctg gcggcgcagg tcaaagcgcg tacagctgaa ttgcaggaac
     9301 tggtgataga acaccgacaa gcacgggcgg aagcagaaaa agccagccag gcaaaatcgg
     9361 cgtttctggc ggcgatgagc catgagatcc gcacaccgct gtacggtatt ctcggcaccg
     9421 cacagctact ggcagataac cccgcactta acgcccagcg tgatgatttg cgggcaatta
     9481 ccgactccgg cgaatcgttg ctgaccatcc tcaacgatat tctcgattat tccgctatcg
     9541 aagcaggtgg caagaatgtt tcggtcagcg atgaaccctt tgaaccgcga ccgctgctgg
     9601 aaagtaccct gcaattaatg agtggacggg tgaaaggtcg cccgattcgc ctggcaacgg
     9661 caattgccga cgatgtaccg actgcgttaa tgggcgatcc gcggcgtatt cgtcaggtca
     9721 ttaccaacct gttgagcaac gccctgcgtt ttactgacga agggcagatc gttttacgta
     9781 gccgtactga tggtgagcaa tggctggtcg aagtggaaga cagcggctgc ggtattgatc
     9841 ccgcgaaact ggcagaaatc ttccagccat ttattcaggt aagtggcaaa cgcggcggca
     9901 ccgggctggg gctgactatc agtagccgtc tggcccaggc gatgggcggc gaactgagcg
     9961 ccaccagcac gccggaggtt ggaagctgtt tttgtttacg cttgccgtta cgtgttgcca
    10021 cggcacccgt gccaaaaaca gtcaatcagg cggtgcgtct tgacggttta cgtttgctgt
    10081 taattgaaga taacccgcta acccagcgaa ttaccgttga gatgctgaac accagtggtg
    10141 cgcaggttgt tgctattggc aatgccgcgc aggctttaga gacactgcaa aatagcgaac
    10201 cgtttgctgc cgcactggtg gattttgatc tgccggatgt caacggcatt acgcttgccc
    10261 gacaactggc acgacaatat ccgtcgctgg ttttgattgg ctttagcgcc catgtcattg
    10321 acgaaacgct gcgccagcgt accagttcac tatttcgcgg gattatccct aaaccggtgc
    10381 cgcgtgaagt gctcggtcag ttactggcgc actatctcca actgcaagcc aataacgatc
    10441 taccactgga tgtatcgcaa ctcaatgaag acgctcagtt aatggggacg gagaagatcc
    10501 acgaatggct ggcattattt acacaacatg ccctgccgct tctcgatgaa atcgacattg
    10561 cccgcgccac tcaggatagc gagaaaataa agcgtgccgc gcatcagcta aaaagcagtt
    10621 gctcgagtct gggaatgcgt agcgccagcc agctctgcgc acaactggag cagcagccat
    10681 tatctgcccc ccttccacac gaagaaatta cacgcagtgt tgccgctctg gaagcatggt
    10741 tgaataagaa agacctgaac gcgatttgaa tcaatatcag ccagttaaga ataactagaa
    10801 tagtggcatc tggtatgcaa cttaacggac tggcaaaatc atggcagaga aaaaaagaac
    10861 ccgctggcag cggcggccag gcacgacggg cggcaaatta ccgtggaatg actggcacaa
    10921 tgccacgacc tggcgtaaag cgacgcaatt attgctgttg gcaatgaata tttatattgc
    10981 catcacgttc tggtattggg tgcgctatta cgaaacggca ggtagtacga tatttgtcgc
    11041 cagaccggga ggtatcgaag gctggctacc gattgccggt ctgatgaatc tgaaatatag
    11101 ccttgcaaca ggtcagttac cgtccgtcca cgccgccgcg atgctgttat tggtcgcttt
    11161 tatcgtcatc agtctattac tcaaaaaggc cttttgctca tggttatgcc cggttggtac
    11221 gctttctgaa ttaatcggcg ctctcggtaa caaactgttt ggtcggcaat gtgtccttcc
    11281 ccgctggctg gatattcctc tgcgcggtgt aaagtatttg ctgttgagtt tttttctcta
    11341 tatcgcgtta ttgatgcctg ctcaggcgat tcactatttt atgttgtcgc cctacagcgt
    11401 ggtgatggac gttaaaatgc tcgatttctt tcgtcatatg gggaccgcga cattaatcag
    11461 cgtgaccgtt ttgctgattg ccagcctgtt tattcgccat gcctggtgtc gttatctttg
    11521 cccatatggc gcgctgatgg gcgtggtttc gctattatcg ccgtttaaga ttcgtcgcaa
    11581 tgccgaaagt tgtatcgatt gtggcaaatg cgcaaaaaat tgcccatcac ggatcccggt
    11641 cgataaatta attcaggtac gaacagtgga atgtaccggc tgtatgactt gcgtagagtc
    11701 atgtccggta gcctcaacat tgaccttttc actgcaaaaa cctgcggcaa ataaaaaagc
    11761 ctttgcgttg tctggctggt taatgacgct actggttctg gggattatgt ttacggtgat
    11821 tggttacgca atgtatgcag gagtatggca aagcccggtg ccggaggaat tgtaccgacg
    11881 cttaattccg caagcgccaa tgattggtca ctaaattagc aacaagcgga acatcacctc
    11941 gcacggggtg atgttcacaa aatcacatta ttttgatttt gacatcataa ctttcaagcc
    12001 cggtcatttt ttctcgcgcg gtgaagcgaa tttcagagac ttctttccct gtattcttgt
    12061 tcagctcggc gattttttgc gcgataaagt cggcgatttc cgtttcggct tgttttttaa
    12121 ctcttcaata ttcactgtta acctcttctg gtcttaaaag acagcaacac gccagtaagg
    12181 tgttggttcc cggtaccctg gtcaggtaag aaatgttata agccatctcc ccgaaagatt
    12241 caataatcaa caggttatcg cttactatac atacagcgtt gaaaaaaaat aagacagata
    12301 taaaaaaggc tccacaaggg agcctgatat tattttgtga aatccagcgg tgcatggtca
    12361 gtgcgtattt gcagcgcgac gttatcaaag gtgatcatcg tcgatttaca aaaaatacac
    12421 ttagcgccca aagggtttcg ctccgtcaca tcgaatgccg atgtgcggta ctgtgaacca
    12481 tgacaacatg gacagcgaaa atggagatgt tgagtaataa cggcaacctt agagcgtaac
    12541 tacgttcgct gccgcggggc cacgttgacc ctgctcaata gaaaattcaa ctttctgatt
    12601 ttcgttcagc gtgcggaatt cattgctctg gatggcggtg aaatggacga aaacgtcttt
    12661 gctgccatca tcaggagtga taaagccaaa acctttatct gcgttaaacc attttactaa
    12721 accagtcatt ttattagaca tattttactt ccttcatttt gggcctttcg gcaaacaggg
    12781 ctttaataca gaaactactt agtgttcagg agagagactc aaaagaaggg gataatgcct
    12841 gataatgaga actgctttag taaactactt tgtatgctgt ctgtctttcc aaccgacgca
    12901 gctattaacg catgataatt gagattaagc aaattttatt ttagccgtcc ggcggtctta
    12961 aatatcgctg gctttatttt attaaccccg tatagtgcag cgcagtattt tcagtaagga
    13021 atttgtttgt cccgtaaaat gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa
    13081 ggattcatta tcccctccga cggtcgtaaa gaagttcagg tccatatttc cgcattcact
    13141 ccccgcgacg cagaagtgct tataccagga ttacgcgtgg aattttgccg cgtaaatggc
    13201 ctgcgaggac caacagcggc aaatgtttat ctctcttaat atgacgctgc ttgtttaaag
    13261 ctgactggtt ttattggcat agttcaatag tcaacaccat gtattataac acaagttact
    13321 ttttcaccgg aaaactaatc cat
//
LOCUS       DAZFKY010000133        13007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFKY010000133 DAZFKY010000000
VERSION     DAZFKY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            76..159
                     /gene="idlP"
                     /locus_tag="ACHKCL_003403"
     CDS             76..159
                     /gene="idlP"
                     /locus_tag="ACHKCL_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO5764299.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            156..548
                     /gene="iraD"
                     /locus_tag="ACHKCL_003404"
     CDS             156..548
                     /gene="iraD"
                     /locus_tag="ACHKCL_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5764300.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(541..1395)
                     /gene="hypT"
                     /locus_tag="ACHKCL_003405"
                     /gene_synonym="qseD"
     CDS             complement(541..1395)
                     /gene="hypT"
                     /locus_tag="ACHKCL_003405"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO5764301.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(1517..2689)
                     /gene="iadA"
                     /locus_tag="ACHKCL_003406"
     CDS             complement(1517..2689)
                     /gene="iadA"
                     /locus_tag="ACHKCL_003406"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5764302.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2702..3163)
                     /gene="yjiG"
                     /locus_tag="ACHKCL_003407"
     CDS             complement(2702..3163)
                     /gene="yjiG"
                     /locus_tag="ACHKCL_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5764303.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(3160..3843)
                     /gene="yjiH"
                     /locus_tag="ACHKCL_003408"
     CDS             complement(3160..3843)
                     /gene="yjiH"
                     /locus_tag="ACHKCL_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5764304.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4093..4643
                     /gene="kptA"
                     /locus_tag="ACHKCL_003409"
                     /pseudo
     CDS             4093..4643
                     /gene="kptA"
                     /locus_tag="ACHKCL_003409"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            complement(4800..10042)
                     /gene="iatD"
                     /locus_tag="ACHKCL_003410"
                     /pseudo
     CDS             complement(4800..10042)
                     /gene="iatD"
                     /locus_tag="ACHKCL_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(10253..11431)
                     /gene="yjiJ"
                     /locus_tag="ACHKCL_003411"
     CDS             complement(10253..11431)
                     /gene="yjiJ"
                     /locus_tag="ACHKCL_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5764305.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            complement(11499..12341)
                     /locus_tag="ACHKCL_003412"
     CDS             complement(11499..12341)
                     /locus_tag="ACHKCL_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5764306.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            complement(12607..>13007)
                     /locus_tag="ACHKCL_003413"
     CDS             complement(12607..>13007)
                     /locus_tag="ACHKCL_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO5764307.1"
                     /translation="FIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVNQANNSTEKQSI
                     DSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQAKNSTEQQDSNS
                     FNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
BASE COUNT         3197 a         3164 c         3259 g         3387 t
ORIGIN      
        1 aggttttata ctttgctgac aggattcagg cctgtctcag actgacatgg atgtaatgaa
       61 caaaagggaa tggctatgga aaatgagcat caatacagtg gtgcccggtg ttcagggcaa
      121 gccgcatatg ttgctaaacg tcaggagtgc gcaaaatgat gcgccaatca cttcaggctg
      181 ttttacctga aatttcaggc aataaaacgt cattgctgcg taaatcggta tgcagcgatc
      241 tcctcactct ttttaattct cctcattcga cattaccgtc attacttgtt tcaggcatgc
      301 ccgagtggca agtgcataac ccgagcgaca aacatctcca gagctggtat tgccgtcagc
      361 tgcgttctgc actgttattt catgagccac ggattgcggc gttgcaggtt aatttgaaag
      421 aagcgtactg ccacactctg gcgatcagcc ttgagataat gctgtaccac gacgatgaac
      481 cgctaacgtt tgatctggtc tgggataatg gcggttggcg cagtgcgacg cttgagaatg
      541 tcagctaagc acaatctcca gctcgcgcag ttcacgccag aagcgttcgg caactggatt
      601 catgcgggta ttcatccggt atgcgtaggc ctgaatcggg atcaccagtt cgtcccgatt
      661 cagcacaacg agctgcccgc tgcgaatttc ttgttggatg gcatactccg gcagccaggc
      721 aatcccacag ccgtcgaggg caacctgctt taaaagctcg ctcatcgaag agacaaaaaa
      781 ggtgctgaaa cttaactcac tgtggcgcgt cagggtgcga ttaatcaatc gccccatgta
      841 ggagttgcgg ctgtaattca gtaacggaaa gtgtggctgc acgaggtcaa aaagtgcttc
      901 tccatgttcg tcactggcgc agacagggaa caattgagat tcaaataagc gaatgtggtc
      961 aaacggcgct tccagcaaat cttcgtcgtg aaaggaaaaa atacagtcac tttgtccttc
     1021 acgcagttta tcgaccgctt catcgacatc aatggcttca atcgcccagg taaagagcgg
     1081 cggcatctgg ctgataatgg acggtaacag accgagggaa agagagtgtg cagcggctat
     1141 cttgatttta cgttgcgcgt aatcgctgcc gccacgcagc tctgcaaggt tgctttctaa
     1201 ctgttgcaac agatggcgga tctgcgaatg gaagattttc ccttgttctg agagttgcag
     1261 cggcgtcacc tggcggttaa acaattcaac gccaatcgcc tgttccagcg cgcggatgcg
     1321 gcggctgaat gccggttgcg agacgttacg actgactgcc gcctgggaaa aattgcggca
     1381 tttttccagg gtcagaaaat cttaaagcca tttggtttca atattatgca aaaccgcacc
     1441 acagtcatcc attacgtttt actctgtcag ccgcccttgg gggcattcta cgtccattag
     1501 ggcggctgac aaccgtttaa gccgtttcaa acgttccttt cacgcaggct ttgccgtctt
     1561 tgaccatcag tttgccgcga gcgtatacct gctcaatgcg caattctggc gtcatgacca
     1621 gtaagtctgc gtcattgcct ggcagaattt cgcctttccc ggtcaggtta aggaaaccgg
     1681 ctacgctact ggtgagcggg cgcagggcat cgctgatact gaaatcatag tctttaacca
     1741 gcacctgcac ggtttccagc aacgtttcaa aacctgcaac gccgatatgg gttaaattcc
     1801 cttcatcatc gaagaacggc tggctaccgt tgccgtcgga gctgagggtg acgcgtgcca
     1861 gcggaatacc cgcctgaacg gcgcggacaa taccttcggc aggggcgacc ggttcgtcaa
     1921 tgctgctggt gatatcgatg gtgccgcctt tgcgcgcgaa ctccagcgcc tgctcaaaca
     1981 acggtacgtt gcggttaacg tgggtcggca gcagcttgct gatcggcaca tcgcagtttt
     2041 ccagcaggtc ataaataggc tgtaatgcct ttttactgtc gcccatgtgg aacacggtga
     2101 cgccaggttt accgccgagc aaaccgccaa cgcgggattc tgccgccata ttagccaggt
     2161 gataaacgtc cggtgcggca gaacggtgat cagagatggc acatttcacg ccaatcacac
     2221 gatcgataat cgccacgtct ttttccacgg aacccgtaat ggtgcgggaa gggacatgat
     2281 aagcgccggt cagcatccag gcgctgatgc cttcttcatt gagcgcacgg gtcttggcga
     2341 gcagggattc cgggtggcga gagatagagt cggtgcccag cagaccaacc actgacgtga
     2401 cgcccgcttc cgtcaggcga cttagcgcca cttccggcgt gcgcgtcgtg ggacctgctt
     2461 cgccgccacc gccaatcaaa tggacgtgtt gatcaataaa acctgggcag aggatctgcc
     2521 cgctgagatc gacaaccgtg cagtccggta caatgtcaga agggatatta ctggcaacgg
     2581 cgataatttt gccgttagcg acgaggacat cgcaaattcc ccgatcttcc ggcgcataca
     2641 aatgcgctcc ctgcagcagg gtaaaaccgg ctgcggtata atcaatcatg gtaactcctt
     2701 gttaaacaat aagctgcata acccagatcg acagcaatgc gttaatcacg cagaccgtaa
     2761 tgatatgcgg gtaatatttg gcattcactt cggctgtccc caggcagcga ccgacatttt
     2821 gcaccgggtt gcccattaaa tacatcgcgg gcagtaaaac ggtgacatcg tgaccggtta
     2881 atgcgccagc ggttgccaga ctggcggcaa caccgactgc gccgcccata ctcattaatg
     2941 cggctaaaag gacggttgcc gcttcaccgg gtaatcccca cagcgccatt acgggttcac
     3001 agatatgccc cacccagtcg agcaggccgg taattttcag cgcctgaatg atgacaaacg
     3061 ccatcaccac gtttggcagc aggttggtcg tggcgatagt aaagccacgg cgcgcgccat
     3121 cgataaacat atccatgacg tttttgcgga cctgagttgt catgcttgcg ctccttgcgt
     3181 tgggttgcga cgttcttcga agttaagcca gacgcgcaaa atgttggctc cgacaaattt
     3241 aaacaccagg atgacggcta aaggtacgat tacggacgtg cccagaaagg cgaacaccgc
     3301 gacgccggaa gagaagtagt tggtgatgat ggcgctaccg ctggtttgat atgcggcgaa
     3361 aatcacttta tcgcgttcgg taatttcacc ttcctgcgcc agctctttgg tcataccggc
     3421 ggcggcatca gtgttttgca agttagcgat cagcgccagc gagcagatcc ctggaatgcc
     3481 caacaacggt tttagcacag gtgtcattaa ctgctgcgcg gcgcgtaatc caccaagacc
     3541 atcggtaatt gaaataatcc ccagagagag aatgactgac ggtgccagct ccagcgcaaa
     3601 gagaaagcca tctttagccc ccgcgccacc cgcgccgcga aatgaggtgg tggctccatt
     3661 cgcaccaggc agttgcccaa acgatccgtt cagaacggaa aaatcaaaaa cgcgccacca
     3721 gctgtctgtt ccggaaaaaa caccggaaaa aaagattatg gtgagaaaaa aagccagata
     3781 ccctttaatg cccacttttt ctgtcgccag ctccccggct acagcatccc cttgttgtgt
     3841 cataacaatc cccattgttt ttttgtgttt gtgtgttgct tgagccagga aaattcctgg
     3901 aacttaaaaa ccttatcagc catgtgggta tggggaaaat gcaagtatgt ttctggctat
     3961 gcgttttttg cataggggaa aaattacgct gtgtggaaaa ggttctgcgc aggattgagt
     4021 tgcagaaaat tcataaaccc agtgacaatg acattggtaa ataaaaacag aaaatccagg
     4081 gacgaaaacg cgatggcaaa gtacaacgaa aaagagctgg cggatacgag caagttttta
     4141 agttttgtcc tccgacacaa accggaggcg attggtatcg tactggaccg tgaaggatgg
     4201 gcgaatatcg ataagctcat actttgtgcc caaaaagcgg gcaagcgatt aacccgtgca
     4261 ttgctggata ccgtggtcgc aaccagcgat aaaagcgttt tagttattcc agtgatggta
     4321 ggtgcatccg cgcggttcag gggcattcga cttcgcaggt cgctatttcc tttgccgaaa
     4381 aaacaccgcc acagtttctc taccacggta cggcaagccg tttccttgac gagataaaaa
     4441 aacaggggct gattgcaggg gatcgtcatt acgtgcatct ctctgctgat gaagcaacgg
     4501 cacgcaaagt gggagcaaga cacggatcgc cggttgtctt aaccgtcaaa gcacaggaaa
     4561 tggcgaaacg aggcatccct ttctggcaag cggaaaacgg ggtctggctg acatcaacgg
     4621 ttgctgttga gtttcttgag tagtcgttta tgccggctgg ggtacaaaaa caataaattc
     4681 aatatattgc aggggttata taggcctgat aagcgcagca catccggcaa tgtagcgttt
     4741 gttatcagtc tgaaaggata ccctcgtaaa agggtatcct ttaataacaa ctgctgaaat
     4801 taaagtgatt tacggcaggc ggtcagttgg gggctggaat cagcgtcgct tccggtcata
     4861 ccggttagca tctgcacata gtagtgccga ctgtaatcat cagattcact tgtccagtaa
     4921 gctcccggtg taaattgcga attactggaa aacgctccaa cgttccccca ttcaccatac
     4981 agtgatccct gcgtgcgttt gttttcgcca gacgtcaaat gtgtgatgcc aggaagatta
     5041 tatccatcat ttttagcatg acagagcgca ttggcagcta gccagatatt ggtatgattt
     5101 ccattattca cccaccagcc tttcagctta attaaggtct ggtaggttgt acctccttgt
     5161 cggggaacag cgcttattgt gactgacgtt tgatcgccga tatttgcgaa agaaacttta
     5221 cccgaagcat cgacagacac ccaaccctgt gaacttgaaa acatataatc atcaacggtt
     5281 ttacccgctt caaagttatc attgttgagt gtgtaatacg ctcctgcaaa gccttcgctc
     5341 gggaatttag ccgtggagaa ggtatggttg tttgcagtga catgaccgga catttctttt
     5401 atcgattgga tgagattcag cgatatgctt aagtttgcct gatgaacccc gttcagcatg
     5461 ggaatgagtg tcgctgttcc ctcttttccg ccagtgacag tcacggtgta ctcgccataa
     5521 tttctttggc tgtaatcaat atcgctcaac ttgagaaagt tcacgaaggg gcctgactgg
     5581 acaaattcca gatgatcaga cgtcactatc gggttaccgt acttgtcttg caatgttagc
     5641 gtaagttttg cgctctgctg gtagtcagcc ttcagagtgt caacatcgga tttcaaaatt
     5701 gacttacttt catcggccgt gccggcaacc aatgtaatgg ttgcggattg gttggctgtg
     5761 ccaggaactt gcacattgac tgttaaggtt tctgccaatg tactggttat tgtcgccgtg
     5821 acttgcccgg actgatcggt acgcgtagtt ggaatattaa ctaccgggct gcctttggtg
     5881 tctaagccat gactaatatc gacaccttca accgggaatc cgtaatcatc ttctactcgt
     5941 gaagtgatgg tgctgctatc gatgccgtcg gcggtgatgg tgtcaggaga agccacgacg
     6001 ctcacaacat gtgccgtttt agcatctgcc ttaaccgcaa ccgttacctg gctctgaacg
     6061 ggaacatctt tgcccattat ggccgtaacg gtatagacat tcactttgtc actggtcagc
     6121 gtcactttgg cctgacctga ctggtcagta aggacttccc ctgtattgct tagcgttgca
     6181 ccttcaggcg ttacgctaaa ggtgataatt tgccctggca tcaattcacc ccgcgcattt
     6241 ctcgccgttg cggtcagcgt aataccctca ctattatgga ccgtagtttc tggtttcgat
     6301 gatgttaact cgagtttgcc atttgccaca ttcacgtcaa ccttactact actgttattg
     6361 tccagcgttg ccgtcacctg ataaacccca ggttgggtgc tgagaaggtt aacgtacgca
     6421 taaccgttat tatcggtgta agccgtatcg cctatcgtaa tgttagttgt tggctgcgca
     6481 cttaatctga cgcgttgacc cgcgattaca ttgttaaacg cgtcctggac gagtatccgt
     6541 agcgtgatgt tttcgcccac cgttatcgtt ttttcggctc cggttatact tattaccttc
     6601 gccgaacttt catccggtag cactgataaa ctaaacgttt tattatcgga agcttgcgct
     6661 ttccggctca ccacgacgtt atgttgtccg gcaatattgc tggtcattgt taccgtcgcc
     6721 tgacccagcg tattggtggt gacttctgtt tcgcttagag tgacatcgtt actctggaaa
     6781 gtgaccaact ggtctttaag cggattttgg taagcgtcca ggatcgttgc cgtcagggta
     6841 ctccccgtac tgttacccgc aacaatggaa tttaatgagg cagtcagcgg ctggagctgc
     6901 gcactgtttg tatccccaat aaaagtgact tgctgataag caacggtatt atttctggtc
     6961 agcgtcgccg taactgtaaa ctcacccgct tttgtgcctc gcaatgtgac cacggcatca
     7021 ccgtttgcat cggtgcgggc ggtttcacta cttagcgtga agttcccggt catggaggct
     7081 ggcaaggtaa atttcacatc ctcatccggc aacgggtgtc cataaacatc tttcacactg
     7141 gcagtcaggg taacgatatt tgtattgtta gcgatcgcac tgcccgaccc ggcattcaag
     7201 gtcattaccg attgttccct gtttgggacc acggtgatcg tgaccgactg gtgaatattt
     7261 ttatccacct caagcgaggc atcgacggaa tagctaccag cttttgaact ggtcaggtgc
     7321 acccgcacaa taccggactg gtctgttgtg gcggtcgtgt tgtccagttg cgagccacca
     7381 ggtgccacaa aattcacttt catatctttt aacgggttgt tatgaaagtc tgtgatgatg
     7441 gcggtcagag tactgccctg gctgttgcca gcaataatgg tatctggatc ggcaaccagt
     7501 tgtttgaatt ttggcgtact cgaatcgcta acgaatttcg caattatccg ttgtgaaaaa
     7561 ccatttggca gcgtggcgct aatcgtattt tcttccgcaa cctgacttac catgtttatt
     7621 gtggcaacac catatgcatc cgtattttgc tgcggcggtt cgacaaactc cgcgcttcct
     7681 ttttcattct taaaggtgac ctgatgattt gtcaggggat tatcactctc atcagtgacg
     7741 ttgatcgtga gggtattaaa tgtcttttta tcggcgtaaa cgtcattatt tgtcgctgat
     7801 aatgtggcaa cgcgagcctt attctggttt gcctcaatgt tataaatatg cgattgcagt
     7861 ggagcattcc agttgtgaag gctaagttgt gcccttagtg cagtcccttg cttataggcc
     7921 ggataattcg cggcataaac ccccggctgc tcttcgtgcc agacaatcgt ggcgccaggt
     7981 ttgctgtttt cgacagtaac ggcttttacc aattcctctt tttgatatgc aaccggttga
     8041 ttgctatcgt ctttcagttc caccctaacc ttgatgtcgt ctccggcata atacgatacg
     8101 ttatcaattg ttattgatga atggtcgcgg gatgagacaa cagggacgat attaacgacg
     8161 atggattctt ttaccgcact ctcaccgtta atttgcggcg tcagtgttac tgaacctgat
     8221 gtcgttccgg cggtcagtat ctgtgtgtaa ctaccatcac cgttatctgt ccagtcagac
     8281 agggtgatat cctgaacgcc ttcactgcgg gtttgcagcg ccagccccgg caccggagtt
     8341 ccgtcggaat cgtgcgcagt aacggtcaat gtggtcgtcg attttttatc tgcagccacg
     8401 gttagcggat tgacggataa cagagaatct ttctggctta atgtcggagc ctgaataact
     8461 accatgcttt gttcatgccg tgacaggtta ccttttacat cctcggcggt aacgtctatc
     8521 gaccatgtat tatcggtttc tggggtgtta gtgaagcggt aacctggcag cgtgaccgtg
     8581 atatcttttc cagacgtgct gactttacct ccggcagctt ccagcgcagc ggcttcgatg
     8641 ttatagcctt taagggcata tttggtctgt agcgaggaaa ccagcggttt tatttctcca
     8701 gacttccctt tcaccggatc cagcagactc aggcgaatca gctctttctt gcggtattcc
     8761 agaacgatgt tgttgttgcg atcaatcagg tcataacgac taccggccag actgcgccgt
     8821 ccggccactt cgtccggatt aagctgtttc tgcattgaac tgctgggttg ccaggtcaga
     8881 tcaacggcaa aacgtgtgtc attttcacct tgcttcccct gacgctgttc cgcactgaga
     8941 gtcagaagcg ggaagggggt atagttgagg cctgcggtaa tagcatgggg gttactttga
     9001 cgatcattct tgtcaaacag cgccacttca tcgccatagt attgttcata gaccagtttt
     9061 ccccccagtt gtggccaggc aggtaaccag ccttccgcgc gtaaatccca gccgttggcc
     9121 gggcgggctt cgaagtcgtt atccaattct ggtgcgctac gccagccggt caggccgata
     9181 taagcgttgc tgctcaactt cagataatca cgccagtatt ctgcgccaag ccctgcgcgg
     9241 gagtgatagc ggctcaggtc gtggtcaaaa aaaaggttga tgcctgacat ccagctggag
     9301 gtgaaatgac gccagcccaa accggtgttg atctgggtac gatcgtctgt tcggtgaagg
     9361 gtatgctggc tgaagagcag ataatcaggt gtgtcatacc acggatgcag gaagtcgaat
     9421 tgcgaatttt tcaggctaaa atcttcatcc acacccagag agattctcgc agtaccaaag
     9481 ttatttaacc aatcagtcat cgcgcctgag gcttctgaag acgcccaacc acgcgccatg
     9541 ccgctggcct gctcactatt catatcttgt gaaagcagag tgccaactcg ctgcgaggtg
     9601 ctggctattt gattctccag cgtgttttcg ccattcgcag gcggtacggc attttgttgc
     9661 tcatggcttt tctgcgaggt tgttgccgga acatccagtt cttcaccctg gcgcacgtta
     9721 tcaaagccgc gagcaaaagt acggaactga ttaagacgac gaagctcctc cagtgaaata
     9781 ccgaagcgat tagcaacgct ttgcgctgat tccagcgcac caagcgtgta aggcacagta
     9841 ttattttcta gtagaggggg ggggcagatg aaactgtcgt ctgaggttta gcatgtacca
     9901 cattttgtgc cgtcgccatg acggggaaaa aaaattgtgt aaataaacaa actccggcgg
     9961 tcaggcggcg tattttgtca cccagaccac ataaagagtt ttgatcatta ttttttattt
    10021 gatgcgcatt tgtcgaagcc atagtgacct tccgtgaaaa aacagaatta aacttgtttt
    10081 ttctagcacg gagatataat gcgatcctga ctataatatg accagaaata atgaattagc
    10141 cgtggaattg ctaaaccagg aataataccg gcggtggctg agtacctcca aagaaaaagg
    10201 atattttaat tattttacgt gatgtgctga aaagttaaat tagttaaatt acttaagaat
    10261 aattcgtatg caatttgaat atttgcatcc cgcagattaa tgctgcgata aatagcgccg
    10321 ctgcgccgca gagtgtggcg ctggcgagcg ccgacgttcc gttgccgagc atactggtca
    10381 gcgccgggcc aagaatttgc ccgataccat aaatgagtgt cacaaagccc aaaaggttaa
    10441 gatttcccgg cacgctaagc tggcgggcga tggtcatcac cagcgaggtc gttcccataa
    10501 aggtgccgcc aaaaccaata ctgctgatga tgagtaataa aggagagctg ctggcgaggg
    10561 ttaacagcac gcagatcgcc tgcaccagca aattcgcggt caggcaaggt aatgctcccc
    10621 accgtttggc tgcccacagc cagccaaagc aacctgggac aatcgacaag ccaaccagtg
    10681 tccagaggtg agccgtcaac acaggctggc ccgcgtcttt cgccatgagc ggcaggtagg
    10741 tggcgacgat gatataacca aaacccgcca gaccatacag aatcgccagt aaccaccagc
    10801 tcatgggttg ttgcgcgatt tttgccaatg gcgctggcgg gataacgtgt ttattcgacg
    10861 ggatgagcag cgccagagca agcaatataa tggcagaaag tgctccggca ccttgccaca
    10921 acgtttgtga agagagggca aaatgcaggc ctgccagcac atattcatta cccagagcga
    10981 tgccgacgcc aacaccagaa aatagcgccg caaggacaaa gggatggcga gtatgttgca
    11041 tgatgagtgt tgagccgaaa atcaacatcc cggcgctggc gactcccgcc agaaagcgaa
    11101 tgatgaaaac cagaagaaac ggcggcagcc acgccatcgc gaggattaat aatccggttg
    11161 cgagggcgga agctaacagg aacgggcgca ggcgtgaggg taaatgaaac gcgccgaatg
    11221 aaaacagcag gctccccgcc agatacccgg catagttacc actggcgatc catgagagtt
    11281 cgctaaatga aaactcgcct tccgccagca tgactggcag catcggcgta tagagaaagc
    11341 ggcctaaacc catacctaag gtcagaacca gcatcccgaa taacgcggtg ctgaaagaaa
    11401 aacgttctac gggatgcgat gtcgaattca tgttctgcct ggtttgtatt attttgagtt
    11461 aacaagttaa taacaaaaaa ggagaacctg ccagactgtt atcgtgattt tgtcgacgta
    11521 aagcgataaa aaagattcgg ttcgccaacg atatagatgt tgccgctatc atccatcgct
    11581 atcccttccg cctgcttgat gttgtgtgaa aggccatact tacctttggt taacgacatt
    11641 tcgccgatca cgtcacctct cacggtcact tcctgcaggg cgcgtgactc atgtgacaac
    11701 accagcaacg tatttttctg tgggttaaat tccgcgccag aaacatcgtc taaagtaaac
    11761 tgacgctgca acgttttatc tttgctgata tgcaactcat cgctacgcaa tagcccggtt
    11821 actttgtaga cctctatcgg atttttctct ttaaaaaacc agaaggtatg gtcctgagag
    11881 gagtaagcca gcccttcaaa gccacagtta gttggtgttt cctgcaatgg gattttaatt
    11941 tttttgagga tgctcacttc cgagtcagcg ttaagtgaaa tcacataaat ggcgtagtca
    12001 cgttcatcac tgatgacaaa tttattatca ccaatgtatt caatcgtctc gagatcctta
    12061 acaaaatcga gcggaatggt gcggatcaag tcgccctcgg tcgtcatttc aacgatggtt
    12121 gcaggtttgt tgatagtgct aaataaggta ttgctctgcg ctgaccaggt cagtgaggag
    12181 atatttttag ttactcctgt aatctcttta ccatcgatag tggcctgata attctgaaac
    12241 cctgcgacat gctggctttt acggacgcag gattgcacaa aaaacaggct tgccgccacg
    12301 atggcgcaaa gaaaaacgat aaaggtgatg cgctttttca acttattgct ccttattatt
    12361 gcttttttct cgtcaaattc agtgcgacag ataaggagta tacggatgaa gcttaatgaa
    12421 ttcttaacgc gggcgatgaa atagtaaacg ccgcaggggg cggcggttgt ctctggatgg
    12481 cagaacattt tcagcttaca tcgactgata aatgcgcctc atctatgaat gatagatgag
    12541 gcggttaacg tttaaatgtt aatctatatc gtctgccata cccactctgt ttgggtgatt
    12601 aatgaactat tcttcaacta tatatgttgg ctgataagac gttaaacccg gaatggaaaa
    12661 tctcctattc acttctttct ttaaacgggc ttgattaaaa ctgttactgt cctgttgctc
    12721 ggtactgttc ttggcttgtt tatcggtact gtcattgacc tgtttatcga tattgtcatt
    12781 ggcttgttta tcggtactgt cattggcttg ttgcaccgta ctgtcaatgg attgttgcac
    12841 cgtactgtca ttggcctgtt tatcggtact gtcaatggat tgtttctcgg tactgttatt
    12901 ggcttgattc acgatattat cattgttcag atatgctccc tcaagataga catttttcgc
    12961 tctgacattg taaaaacagg tatttttaaa aagcgttttt ataaata
//
LOCUS       DAZFKY010000134        12861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFKY010000134 DAZFKY010000000
VERSION     DAZFKY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(402..488)
                     /locus_tag="ACHKCL_003414"
     tRNA            complement(402..488)
                     /locus_tag="ACHKCL_003414"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(452..454),aa:Leu,seq:gag)
     gene            complement(503..835)
                     /gene="secG"
                     /locus_tag="ACHKCL_003415"
     CDS             complement(503..835)
                     /gene="secG"
                     /locus_tag="ACHKCL_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5764308.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1063..2400)
                     /gene="glmM"
                     /locus_tag="ACHKCL_003416"
     CDS             complement(1063..2400)
                     /gene="glmM"
                     /locus_tag="ACHKCL_003416"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5764309.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2393..3241)
                     /gene="folP"
                     /locus_tag="ACHKCL_003417"
     CDS             complement(2393..3241)
                     /gene="folP"
                     /locus_tag="ACHKCL_003417"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5764310.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3331..5265)
                     /gene="ftsH"
                     /locus_tag="ACHKCL_003418"
     CDS             complement(3331..5265)
                     /gene="ftsH"
                     /locus_tag="ACHKCL_003418"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5764311.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5365..5994)
                     /gene="rlmE"
                     /locus_tag="ACHKCL_003419"
     CDS             complement(5365..5994)
                     /gene="rlmE"
                     /locus_tag="ACHKCL_003419"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5764312.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6120..6413
                     /gene="yhbY"
                     /locus_tag="ACHKCL_003420"
     CDS             6120..6413
                     /gene="yhbY"
                     /locus_tag="ACHKCL_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5764313.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6569..7045)
                     /gene="greA"
                     /locus_tag="ACHKCL_003421"
     CDS             complement(6569..7045)
                     /gene="greA"
                     /locus_tag="ACHKCL_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5764314.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7293..8726
                     /gene="dacB"
                     /locus_tag="ACHKCL_003422"
     CDS             7293..8726
                     /gene="dacB"
                     /locus_tag="ACHKCL_003422"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5764315.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8766..9938)
                     /gene="cgtA"
                     /locus_tag="ACHKCL_003423"
     CDS             complement(8766..9938)
                     /gene="cgtA"
                     /locus_tag="ACHKCL_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5764316.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9954..10919)
                     /locus_tag="ACHKCL_003424"
     CDS             complement(9954..10919)
                     /locus_tag="ACHKCL_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5764317.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11046..11303)
                     /gene="rpmA"
                     /locus_tag="ACHKCL_003425"
     CDS             complement(11046..11303)
                     /gene="rpmA"
                     /locus_tag="ACHKCL_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5764318.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11324..11635)
                     /gene="rplU"
                     /locus_tag="ACHKCL_003426"
     CDS             complement(11324..11635)
                     /gene="rplU"
                     /locus_tag="ACHKCL_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5764319.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11894..>12861
                     /gene="ispB"
                     /locus_tag="ACHKCL_003427"
     CDS             11894..>12861
                     /gene="ispB"
                     /locus_tag="ACHKCL_003427"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5764320.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
BASE COUNT         3172 a         3569 c         3024 g         3096 t
ORIGIN      
        1 tcagacatgt ttttcaactc tgatccatat cagaacgaaa cactgttagt tttttctttt
       61 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
      121 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
      181 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
      241 agcagtaggg aaaagtttat aacgaaggcg taccggcacc atcgtttcaa ggtaccagct
      301 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
      361 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
      421 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
      481 acctcggcac ggaaactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
      541 ctggctgagt ttgttcggtt ttcgccggcg cactcaggtt ttcccattcg ctacctttat
      601 tggtcttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
      661 gcaacgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
      721 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgt agcatgatca
      781 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
      841 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagtttata
      901 atctttccca ctgaagcggg tgtgaatact aaccaaaacg aatgaccttc gcaagggcaa
      961 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgccgagaaa
     1021 aaaggcgaca atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc
     1081 atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac
     1141 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     1201 cgtttcaact tctgcggtca ccgctttgac tgattcatgc tccagcggat cgccgctacc
     1261 tgcggtgtaa cgcacgttta ccaggatttg cgggaacatt ttcatgccgc tgcaaaggtc
     1321 gtgcaggctc atatggttac gcgccatcgc cgccagcacc tgcaaaccag caacgatgcc
     1381 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     1441 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     1501 tggaattcct aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac
     1561 agcgccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     1621 atcgccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     1681 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     1741 ggtagccccc acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac
     1801 attcgctccc agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     1861 atccaccaca atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     1921 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     1981 aacacagctg atctcttttt ccatttccgc ttcgatggct tcttcaactg catccggcag
     2041 cttagtaccg tcaatagaaa agaatttaat gccattatcg tagaacgggt tatgcgatgc
     2101 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     2161 tggcatcggg ccagtgaaga gtgcggaaag gcccgcagcc gccagccccg cttccagtgc
     2221 tgactccagc atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc
     2281 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     2341 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     2401 agcgtttgtt ttcctttgca gacagagtag cttccaccac tcgcatcgct tctacggttt
     2461 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     2521 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     2581 ttcgtgacat acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     2641 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     2701 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     2761 cttctgcaaa gacatcgtca tacttcgggg cttcctgcat ggtttttgga tttccctgca
     2821 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     2881 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     2941 ctggtttgga tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     3001 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct ggccgcgtgg
     3061 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     3121 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     3181 ggatccccat tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca
     3241 tgatgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     3301 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     3361 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     3421 gtcgccagca ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     3481 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     3541 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     3601 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     3661 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     3721 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgtgcca ggttggtcgc
     3781 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     3841 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     3901 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     3961 cgtcactttg tgcaccggat cgtgctccgg caccaggcga ccgataatcg catgacccgc
     4021 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     4081 acccatcatg attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     4141 gccacgagca gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa
     4201 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     4261 catgtgaacc ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     4321 acggtcgaaa cggccaggac gcagcagcgc tgggtcgaga acgtccggac ggttagtcgc
     4381 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     4441 cagagtctgt tcacgttcat cgtgaccacc gcccagacct gcgccacgct ggcggcctac
     4501 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     4561 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     4621 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     4681 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     4741 gctcggctcg cgcagatact caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     4801 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttactctt
     4861 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     4921 gacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggctggg
     4981 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     5041 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     5101 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     5161 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     5221 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     5281 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     5341 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     5401 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     5461 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     5521 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     5581 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     5641 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     5701 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     5761 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     5821 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     5881 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     5941 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     6001 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     6061 tatatgggag atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga
     6121 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     6181 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     6241 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct
     6301 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     6361 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     6421 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     6481 tgccacgctc tgttcgttga taaaaggccg ctatgcggcc tttttccttt ctttacaata
     6541 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     6601 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     6661 cacgagcaat aggtgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     6721 caacgatgcg ataagtctgt tcttcgtcag aatcgagatt cagcaccgtt acggtagcac
     6781 caaaaataac gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca
     6841 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
     6901 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
     6961 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
     7021 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
     7081 tgttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
     7141 aaaaaatacc gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat
     7201 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
     7261 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
     7321 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
     7381 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
     7441 ttgattacca cagtcagcag atggcgctgc ccgccagtac ccagaaagtg attactgcgc
     7501 tggcggcgtt gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag
     7561 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
     7621 cgttaaaacg tcaagatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
     7681 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
     7741 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgtc gccatagttg
     7801 accgcaactg tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta
     7861 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
     7921 gttctgccga agcgcaatac tgcgaactgg atgtggtgcc aggcgacctg aaccgcttta
     7981 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
     8041 atggagccag ctatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
     8101 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
     8161 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gacaacatga
     8221 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc
     8281 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
     8341 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
     8401 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
     8461 tgccgctagc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
     8521 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
     8581 tcattaccac agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag
     8641 tagaaccggc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
     8701 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt
     8761 gctgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca
     8821 tcccagtcct catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc
     8881 cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca
     8941 atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag
     9001 atcagataat atttatcttc ccagcccagc gcctctgcga tcgctttcgc tttctcttcg
     9061 gcttctacct tatccagcag atcgatcttg ttgaagacta accaacgcgg tttcgccgcc
     9121 agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg
     9181 gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc
     9241 ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata
     9301 tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga
     9361 gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc
     9421 gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc
     9481 agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact
     9541 gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc
     9601 tgaccgtgtt tggtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta
     9661 cctaccggca ctttaattgt gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg
     9721 ccattctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc
     9781 aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg
     9841 ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct
     9901 gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg
     9961 cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga
    10021 ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg
    10081 tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac
    10141 agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc
    10201 atcgcttccg ccagggcgcc atatcctacc aaggtattca gtccgcaaaa aattaaacat
    10261 gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca
    10321 attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa
    10381 accttttgcg ccacgccata actcacccaa accgtcgcag caccgacccc aaagataact
    10441 ccccaggtgt aatcggttag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc
    10501 accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc
    10561 aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa
    10621 gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg
    10681 gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc
    10741 aacctcttct tcaccgcaag aatggccccc aggccaatac tcgccatcaa gaaacggtaa
    10801 aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcaat tggcaacgcc
    10861 ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata
    10921 ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt
    10981 tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga
    11041 aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat
    11101 ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg
    11161 ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg
    11221 ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag
    11281 ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag
    11341 taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac
    11401 gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga
    11461 ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca
    11521 gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg
    11581 tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa
    11641 actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag
    11701 ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa
    11761 aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcacccta
    11821 tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag
    11881 cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg
    11941 tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg
    12001 ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc
    12061 acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat
    12121 ccacacggcg actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa
    12181 agctaccgcc aacgccgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta
    12241 tacccgcgcg ttccagatga tgaccagcct cggttcactc aaagtgctgg aagtcatgtc
    12301 agaagccgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaatg ttaacgatcc
    12361 ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga
    12421 ggctgccgcg cagtgttccg gaattctggc gggctgtacg ccggaggagg agaaaggcct
    12481 gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta
    12541 caatgccgat ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc
    12601 gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg
    12661 taccgccatc gaacagggta acggtcgcca tcttctgaaa ccggttctgg aagcaatgaa
    12721 cgcttgtgga tctcttgaat ggacgcgtca gcgcgccgag gaagaagcag acaaagccat
    12781 cgcagcgtta caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca
    12841 catcgctgtt caacgcgatc g
//
LOCUS       DAZFKY010000135        12839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFKY010000135 DAZFKY010000000
VERSION     DAZFKY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            251..1765
                     /gene="caiT"
                     /locus_tag="ACHKCL_003428"
     CDS             251..1765
                     /gene="caiT"
                     /locus_tag="ACHKCL_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5764321.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1796..2938
                     /gene="caiA"
                     /locus_tag="ACHKCL_003429"
     CDS             1796..2938
                     /gene="caiA"
                     /locus_tag="ACHKCL_003429"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5764322.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3067..4284
                     /gene="caiB"
                     /locus_tag="ACHKCL_003430"
     CDS             3067..4284
                     /gene="caiB"
                     /locus_tag="ACHKCL_003430"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5764323.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4358..5911
                     /gene="caiC"
                     /locus_tag="ACHKCL_003431"
     CDS             4358..5911
                     /gene="caiC"
                     /locus_tag="ACHKCL_003431"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5764324.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6020..6805
                     /gene="caiD"
                     /locus_tag="ACHKCL_003432"
     CDS             6020..6805
                     /gene="caiD"
                     /locus_tag="ACHKCL_003432"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5764325.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6811..7401
                     /gene="caiE"
                     /locus_tag="ACHKCL_003433"
     CDS             6811..7401
                     /gene="caiE"
                     /locus_tag="ACHKCL_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5764326.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7520..7915)
                     /gene="caiF"
                     /locus_tag="ACHKCL_003434"
     CDS             complement(7520..7915)
                     /gene="caiF"
                     /locus_tag="ACHKCL_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5764327.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8176..11397)
                     /gene="carB"
                     /locus_tag="ACHKCL_003435"
     CDS             complement(8176..11397)
                     /gene="carB"
                     /locus_tag="ACHKCL_003435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5764328.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11415..12563)
                     /gene="carA"
                     /locus_tag="ACHKCL_003436"
     CDS             complement(11415..12563)
                     /gene="carA"
                     /locus_tag="ACHKCL_003436"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5764329.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3041 a         3326 c         3371 g         3101 t
ORIGIN      
        1 tgctattttt ttactcgcaa cataacataa cataacaaac tgattaataa tcaaaattaa
       61 cgagaaaagc gccctctatt cacccgaaac aaaaatgtga taacaatcac agaatgcagc
      121 ttattgaata cccattatga gttagccatt aacgcgtcca tgaggttaat aataattata
      181 ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat aaatattcgt tttcacagtg
      241 gaattaactc atgaagaatg aaaagagaaa aacgggaata gaaccgaagg ttttctttcc
      301 gccattaata atcgtcggca tactttgttg gcttacagtc agagatctgg atgcggcgaa
      361 tgtcgttatt aatgctgtat tcagttacgt caccaatgta tggggatggg catttgaatg
      421 gtatatggtg gtgatgcttt tcggttggtt ctggctggtg tttggcccgt atgccaaaaa
      481 gcgtttaggt aacgaaccac ctgaatttag taccgccagt tggatcttta tgatgttcgc
      541 ctcctgtacg tctgctgccg tactgttctg gggatcgatt gagatctact actacatctc
      601 caccccgccg tttggcttag aaccgaactc gacaggagcg aaagagttgg ggctggctta
      661 cagcttgttc ctctggggac ctctgccgtg ggccacttac agcttccttt cagtcgcctt
      721 cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc cccagctcca ccctggtgcc
      781 gctggtaggt gaaaaacacg ccaaagggtt gttcggcact atcgtcgaca acttctatct
      841 cgtcgccttg atcttcgcga tgggtaccag tctgggcctt gccacgccgc tggtgaccga
      901 gtgtatgcaa tggttgtttg gcattccgca taccctgcaa ctggacgcta tcatcattac
      961 ctgctggatt atcctcaacg ccatttgcgt cgcctgcggt ctgcaaaaag gggtacgtat
     1021 cgccagtgac gtgcgtagtt acctgagctt cctgatgctg ggttgggtgt tcatcgtcag
     1081 cggtgccagc ttcatcatga actacttcac cgattcggtg gggatgttgc tgatgtatct
     1141 gccgcgcatg ttgttctata ccgatcccat cgctaaaggc ggcttcccgc agggctggac
     1201 cgtgttctac tgggcatggt gggtgattta cgccatccag atgagtatct tcctcgcccg
     1261 catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc atggtgatgg gactgacagc
     1321 atccacctgg atcctgtgga ctgtactcgg tagtaacact ctgctgttga tagataaaaa
     1381 catcatcaac attccaaatc tgatcgaaca gtacggtgtg gcgcgcgcca tcatcgaaac
     1441 ctgggccgct ctgccgctca gcaccgccac catgtggggc ttcttcatcc tctgctttat
     1501 tgccaccgtt acgctggtta acgcctgctc ttataccctg gcgatgtcca cttgccgcga
     1561 agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc ggttggtcaa ttctggttgg
     1621 cattatcggt attgttttgc tggcgctcgg cggcctgaaa ccgattcaaa ctgccattat
     1681 cgccggagga tgcccgctgt tcttcgtcaa cattatggtg acgctctcct ttattaaaga
     1741 cgcgaaacag aactggaaag attaattaac ccccaaaata tcaagaggtt gaaagatgga
     1801 ttttaattta aatgatgagc aggaactgtt tgtcgccggt atccgcgaac tgatggccag
     1861 cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac agcgtctacc cggaacgttt
     1921 tgtcaaagca ctggcggata tgggtatcga cagtctgctg atccctgaag agcacggtgg
     1981 tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg gagctgggac gtctgggggc
     2041 accaacctat gtactgtacc agttgccggg cgggttcaat accttcctgc gcgaaggcac
     2101 acaagagcag atcgacaaga ttatggcttt ccgcggcacc ggtaagcaga tgtggaactc
     2161 agcgattacc gaaccgggcg cgggctccga cgtgggtagc ctgaaaacga cttatacccg
     2221 tagaaatggt aagatttatc ttaatggtag taagtgtttt attaccagta gcgcctacac
     2281 cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg gacaaacctg tctacaccga
     2341 atggtttgtt gatatgagca aaccgggcat caaagtgacc aaacttgaga agctcggtct
     2401 gcgtatggat agctgctgtg aaatcacctt tgacgatgtg gaactggacg agaaagacat
     2461 gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa gagttcgacc atgaacgttt
     2521 cctggtagcc ctgaccaact acggtacggc gatgtgcgcc tttgaagatg cggcgcgcta
     2581 cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt ttccagttga ttcaggaaaa
     2641 attcgcccac atggcgatca aattaaactc catgaaaaac atgctgtatg aagcagcgtg
     2701 gaaagcagac aacggcacca tcacctctgg cgatgcagcg atgtgcaaat acttctgcgc
     2761 caatgcggca tttgaagtgg tggatagcgc aatgcaggtg ctgggcggtg tcgggattgc
     2821 gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt gtagaccgcg tttccggagg
     2881 atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg ctgaagcaat accgctaagt
     2941 ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg cctacgggag cacgaatgta
     3001 ggccggataa ggcgtttacg ccgcatccgg cagtcatgcg ctacataaat ttttcaggag
     3061 ctaataatgg atcatctacc catgccgaaa ttcgggccgt tggccggatt gcgcgttgtc
     3121 ttctccggta tcgaaattgc cgggccgttc gccgggcaaa tgttcgcaga atggggtgcg
     3181 gaagttatct ggatcgagaa cgtcgcctgg gccgacacca ttcgcgttca accgaactac
     3241 ccgcaactct cccgccgcaa tttgcacgcg ctgtcgttaa atattttcaa agatgaaggc
     3301 cgcgaagcgt ttctgaaatt aatggaaacc accgatatct tcatcgaagc cagtaaaggt
     3361 ccggcctttg cccgtcgtgg cattaccgat gaagtactgt ggcaacacaa cccgaaactg
     3421 gttatcgctc acctgtccgg ttttggtcag tacggcaccg aggagtacac caatcttccg
     3481 gcctataaca ccatcgccca ggccttcagt ggttacctga ttcagaacgg tgatgttgac
     3541 cagccaatgc ctgccttccc gtataccgcc gattactttt ctggcctgac cgccaccacg
     3601 gcggcgctgg cagcactgca taaagcgcgt gaaaccggta aaggcgaaag tatcgacatc
     3661 gccatgtatg aagtgatgct gcgtatgggc cagtacttca tgatggatta cttcaacggc
     3721 ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct actatgccgg ttgcggtctg
     3781 tataaatgtg ccgacggcta catcgtgatg gaactggtgg gcattaccca aattgaagag
     3841 tgctttaaag atattggcct cgcacatcta ctcggtacgc cggaaatccc ggaaggcact
     3901 cagcttatcc accgtatcga atgcccttac ggcccactgg tagaagaaaa actcgatgcc
     3961 tggctggcgg cacataccat cgcagaagtt aaagaacgct ttgccgaact gaatatcgcc
     4021 tgtgccaaag tactgaccgt accggaactg gaaagcaatc cacagtatgt tgcccgtgaa
     4081 tcaatcactc agtggcaaac gatggatggt cgcacctgca aagggccgaa catcatgccg
     4141 aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc cctcacatgg catggacacg
     4201 gctgccattt tgaaaaatat cggctacagc gaaaacgaca ttcaggagtt ggtcagcaaa
     4261 ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc ggcttgcctg actagcgccc
     4321 gcccaataac aaacgaacaa aaatggatag aggtgcaatg gatatcattg gcggacaaca
     4381 tctacgtcaa atgtgggacg atctggcgga cgtttacggt cataaaacgg cgctgatttg
     4441 tgaatccagc ggcggagtcg ttaaccggta tagttatctt gagttaaatc aggagattaa
     4501 ccgcacggca aacctgtttt atacgctggg gattcgcaaa ggcgacaagg ttgcactaca
     4561 tctcgacaac tgcccggaat ttatcttttg ctggttcggg ctggcaaaaa ttggcgcgat
     4621 tatggtgccg attaacgccc gcctgttacg cgaggaaagt gcgtggatcc tgcaaaatag
     4681 ccaggcgtgc ctgctggtga ccagtgcgca attctatcct atgtatcaac agattcagca
     4741 agaagatgcc actcaattgc ggcacatttg cctgacagat gtggcacttc ccgctgatga
     4801 tggcgtgagt tcgtttactc aactgaaaaa tcaacaacct gccaccttgt gctatgcacc
     4861 gccgctatcg actgacgata cggcggaaat tctcttcacc tccggcacca cctcccgacc
     4921 gaaaggtgtg gtgattaccc attacaacct gcgcttcgct ggatattact ccgcctggca
     4981 gtgtgcactg cgtgacgatg acgtctacct gacggtaatg cctgcgtttc atatcgattg
     5041 ccagtgtact gcggcgatgg cggcgttttc tgccggggcc acctttgtgc tggtcgagaa
     5101 atacagcgcc cgcgccttct ggggacaggt acagaagtac cgcgccacca ttaccgaatg
     5161 tattccgatg atgattcgta cgttgatggt gcagccgcct tcagcgaacg atcggcaaca
     5221 ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag caggaaaaag acacattttg
     5281 tgaacgcttc ggtgttcgct tgctgacgtc ttatgggatg acggaaacca ttgtgggcat
     5341 tatcggcgat cgccctggcg ataaacgacg ctggccgtcg attggtcggg cggggttttg
     5401 ctacgacgcg gagatccgcg acgatcacaa tcgcccgctc ccggcaggtg agatcggtga
     5461 aatctgtatt aaaggcgtac cagggaaaac catcttcaaa gagtattttc tcaacccgaa
     5521 agccactgca aaagtgctgg aagctgatgg ctggctgcat accggcgata ccggataccg
     5581 cgacgaagaa ggcttttttt atttcatcga tcgccgctgc aatatgatca aacgcggtgg
     5641 cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc acccatccaa aaattcagga
     5701 tatcgtggtt gtgggtatta aagattcgat tcgcgatgaa gccatcaaag catttgtggt
     5761 gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttc cgcttctgcg aacaaaatat
     5821 ggcgaaattt aaagtgccct cttatctgga gatcagaaaa gatctgccac gtaattgctc
     5881 ggggaaaata attagaaaga atctgaaata aatgaaacgg cagggaacta ccctgccagc
     5941 taataatcac gctctaaaac aatatgcatt ttttgcaggg atgttgtcgt ccctgaaaaa
     6001 gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg acccgcaatg gatcaattct
     6061 ggaaattact cttgatcgcc caaaagcgaa tgctattgat gcaaaaacca gctttgaaat
     6121 gggcgaagta tttctaaatt tccgtgacga tccgcaatta cgtgtcgcca ttattaccgg
     6181 tgccggagag aaattctttt ccgcgggctg ggatttaaaa gcggcagcag aaggcgaagc
     6241 accggatgct gactttggcc cgggtggttt tgcgggatta accgaaattt tcaatctcga
     6301 caaaccggtt atcgcagctg tgaacggcta cgcctttggc ggcggtttcg aactggcgct
     6361 ggcggcagat tttattgttt gtgccgataa cgccagcttc gccctgccgg aagccaaact
     6421 gggcatcgtt cctgacagcg gcggtgtgct gcgtctgccg aagatcctgc cgcctgccat
     6481 cgtcaatgaa atggtgatga ccggcagacg aatgggcgca gaagaggcgc tgcgttgggg
     6541 gatagtcaac cgcgtggtta gccaggcgga actgatggat aacgcccgcg aactggctca
     6601 gcagctggtt aacagcgccc cgctggcgat tgcggcgctg aaagagatct accgcaccac
     6661 cagcgaaatg ccggtagaag aatcgtatag ctatattcgc agcggcgtgt tgaaacacta
     6721 tccatcggtt ctgcattcgg aagatgccat tgaagggccg ctggcgtttg ccgagaagcg
     6781 cgatccggtg tggaaaggac gttaacaacc gtgagctatt acgcctttga ggggttaatt
     6841 ccggtggttc acccgacggc gtttgtccat cccagtgccg tcttgattgg cgatgtgatt
     6901 gtgggagccg gtgtctacat cggcccactc gcctcactgc gtggtgacta cgggcggttg
     6961 atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta caccgacact
     7021 gacaccatcg ttggggaaaa cggccatatc gggcacggag cgatcctgca tggttgtgtg
     7081 attggtcgcg atgcattggt cgggatgaac agcgtgatta tggatggcgc ggtcattggc
     7141 gaagagagca ttgttgccgc catgagcttt gtcaaagcag gctttcacgg cgagaaacgc
     7201 cagttgttga tgggtacgcc agcccgcgct gtacgcagtg ttagtgacga cgagttacac
     7261 tggaaacggt tgaataccaa agagtatcag gatcttgtcg ggcgctgtca tgcatcgtta
     7321 catgaaacac agccactgag gcaaatggag gaaaatcgcc cccgtttgca ggggacgacg
     7381 gatgtgacgc ctaaacggtg atcttgccgg atgcggcgta aacgccttat ccaactctgc
     7441 tggagagcaa ccgtaggccg gataagatgc gccagcatcg catccggcga ccaggtcgca
     7501 cggtatcatc gaaatgacat taacgacgca tactctttga caacatcatc tgccacttct
     7561 gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggtgtcttt cttacgccga
     7621 ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca
     7681 ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa
     7741 cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc
     7801 tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca
     7861 tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa
     7921 ctccagtttc acaaattaaa agtctggcta tgactcgttt tgaagcaatt aaacaaggcc
     7981 atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa
     8041 atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa
     8101 aacggtcctc atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc
     8161 cgccatgaca cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc
     8221 agtcgcatcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata
     8281 atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg
     8341 gcctgaggtg gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg
     8401 cggacggcct tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac
     8461 aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag
     8521 gtccaccacg cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt
     8581 catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg tgcggcccac
     8641 gcccatgact tccccggtag aacgcatttc tggccctaac agcgggtcaa cgcccgggaa
     8701 tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa cttctttggt
     8761 tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg
     8821 tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt taacttcaat
     8881 caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcaggccgc gcacctgcaa
     8941 ttcgaaggcc agtttctgca cctgctggcg cattacatcc tgaatttcct gacttaaggt
     9001 gtacgctggc agcgaacatg cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc
     9061 catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac
     9121 tgcgtcatca aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt
     9181 ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag
     9241 aacgtaagac ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat
     9301 ttcaatagca gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg
     9361 ctggaagcgt tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg
     9421 tacgccagcg gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac
     9481 gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg
     9541 ctcgaagtag aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt
     9601 aaccataatg gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta
     9661 gtcgaattcg ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc
     9721 acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt
     9781 gtcggtggca aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata
     9841 ttggtcacgc agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc
     9901 atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc
     9961 cacttccgcc actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg
    10021 gtcaatgtta gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc
    10081 gatgtaccag atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc
    10141 ttccgggtca tccaggctca ctttcgggtc gaatccagtc gcaccgactt ccaggccgcg
    10201 cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga
    10261 tttcatctga gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg
    10321 aattttggta accacgtaat cgatggacgg ctcgaaggag gccggagtac gtccgccagt
    10381 gatgtcgttc atcagttcat cgagggtgta acccaccgcc agtttcgccg ccactttagc
    10441 aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat
    10501 ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc
    10561 ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc
    10621 tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat
    10681 cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat cgcgcaccac
    10741 ttccatctcg tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg
    10801 agagagatcc agaccgcggg cgcaaatttc ttcaaactct tcgcggttat aagcgatacc
    10861 gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc
    10921 ggcaaccgcc agcgcttctt ccatcgtatg tgcgataccg gaacgcgcgg tttccagacc
    10981 aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc
    11041 agtggcacca atcatggtga cgccgaactc ttccaacacg ccctgacgtt ccagctccag
    11101 cgcgcagttc agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt
    11161 ttcaataatc ttacgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat
    11221 ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc
    11281 ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat
    11341 aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc
    11401 tcttttactc ctgattactt agcggtttta cggtactgct caattaactc gataaagtgg
    11461 tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg
    11521 aacgccggtt tatcggtgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc
    11581 gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg
    11641 gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca
    11701 aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgaggcag
    11761 atgccgaaga ccggaatatc ggtttcgagg aatttctgga tggcggtaat ggcgtaatcg
    11821 cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc
    11881 acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc
    11941 atccgcagga tgttgcgttt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg
    12001 tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta
    12061 taggcttctg cggtggtcac ttcttttgcc agatccatgc cattcagacc tgggaacgcg
    12121 cgggcttttt ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc
    12181 tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc
    12241 acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca
    12301 atcagcggca ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg
    12361 gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa
    12421 taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc
    12481 ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga
    12541 accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac tttatttgca
    12601 tattaattca caatgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca
    12661 aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata
    12721 ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaagatc atctaacctg
    12781 ttctggaaaa cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggcaaaag
//
LOCUS       DAZFKY010000136        12834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFKY010000136 DAZFKY010000000
VERSION     DAZFKY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            180..893
                     /gene="aphA"
                     /locus_tag="ACHKCL_003437"
     CDS             180..893
                     /gene="aphA"
                     /locus_tag="ACHKCL_003437"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5764330.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1004..1420
                     /locus_tag="ACHKCL_003438"
     CDS             1004..1420
                     /locus_tag="ACHKCL_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5764331.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1424..1780
                     /locus_tag="ACHKCL_003439"
     CDS             1424..1780
                     /locus_tag="ACHKCL_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5764332.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHKCL_003440"
     CDS             complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHKCL_003440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5764333.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHKCL_003441"
     CDS             4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHKCL_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5764334.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5527..5808)
                     /locus_tag="ACHKCL_003442"
     CDS             complement(5527..5808)
                     /locus_tag="ACHKCL_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5764335.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHKCL_003443"
     CDS             6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHKCL_003443"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5764336.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHKCL_003444"
     CDS             complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHKCL_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5764337.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8235..8699
                     /gene="soxR"
                     /locus_tag="ACHKCL_003445"
     CDS             8235..8699
                     /gene="soxR"
                     /locus_tag="ACHKCL_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5764338.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHKCL_003446"
                     /gene_synonym="yjcD"
     CDS             9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHKCL_003446"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5764339.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10745..12394
                     /locus_tag="ACHKCL_003447"
     CDS             10745..12394
                     /locus_tag="ACHKCL_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5764340.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12548..>12834)
                     /locus_tag="ACHKCL_003448"
     CDS             complement(12548..>12834)
                     /locus_tag="ACHKCL_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5764341.1"
                     /translation="ANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASVQGMGISILRGE
                     ENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQHAD"
BASE COUNT         2966 a         3173 c         3387 g         3308 t
ORIGIN      
        1 attttttaca aaaaaacgac aggaacgtat ttactaggtt aaatataatc atcctgcttt
       61 tcatcacaaa aaccgcagat aatccttcct ttcccctgta gctggcgtta tggtcagata
      121 gtttttgcaa caaatctcac aataaaaagt ttcaacatac tgactattta gggaaaaata
      181 tgcgcaagat cacacaggca atcagtgccg tttgcttatt gttcgctcta aacagttccg
      241 ctgttgccct ggcctcatct ccttcaccgc ttaaccctgg gactaacgtt gccaggcttg
      301 ctgaacaggc accaattcat tgggtttcgg tcgcacaaat tgaaaatagc ctcgcagggc
      361 gtccgccaat ggcggtgggg tttgatatcg atgacacggt acttttttcc agtccgggct
      421 tctggcgcgg caaaaaaaac ttctcgccag aaagcgaaga ttatctgaaa aaccctgtat
      481 tctgggaaaa aatgaacaac ggctgggatg aattcagcat tccaaaagag gtcgctcgcc
      541 agctgattga tatgcatgta cgccgtggtg acgcgatctt ctttgtgact ggtcgtagcc
      601 cgacgaaaac agaaacggtt tcaaaaacgc tggcggataa ttttcatatt cctgccacca
      661 gcatgaatcc ggtgatcttt gcgggcgata aaccagggca aaatacaaaa tcgcaatggc
      721 tgcaggataa aaatatccga attttttatg gcgattccga taacgatatt accgccgcac
      781 gcgatgtcgg cgctcgtggt atccgcattc tgcgtgcctc caactctacc tacaaaccct
      841 tgccacaagc gggtgcgttt ggtgaagagg tgatcgtcaa ttcagaatac tgacagagcg
      901 ggagagcgtg acactctccc gaatgctgtt tttttaatca cacctttatc ctttcgctgt
      961 cttgctgcaa actgattaag agagttttat caaggagcag cacatgtggt atcaaaagac
     1021 gctcacgctt agcgccaaat ctcgtgggtt tcatctggta acggatgaaa ttctgaatca
     1081 gctggctgat atgccgcgcg ttaacatcgg cttactgcat ctgttgctgc aacatacctc
     1141 cgcctctctg acacttaatg agaactgcga tcccaccgta cgccacgaca tggagcgttt
     1201 tttcctccgc accgttcccg acaacggaaa ttatgagcat gactatgagg gagcagacga
     1261 tatgccttct catatcaaat cctcaatgct gggaacatcg cttgtattgc cggtgcataa
     1321 agggcgtatt cagaccggca cctggcaagg catttggctg ggggaacatc gcatccacgg
     1381 cggatcgcgt cgcatcatcg cgacactaca aggggagtaa aaaatgacca tttcggagtt
     1441 gctacaatat tgcatggcaa aaccaggcgc agaacagagc gtgcataacg actggaaagc
     1501 gacgcagatc aaagtggaag atgtactgtt tgcgatggtg aaagaagtag aaaatcgccc
     1561 agctgtttcg ctgaaaacca gcccggagct ggcggagctg ctacgtcagc agcacagcga
     1621 tgtgcgtcca agccgccatc tgaataaagc gcactggagc accgtgtatc tcgacggttc
     1681 gctgccggat tcgcaaatct attatctggt ggatgcgtct tatcagcagg cggtgaattt
     1741 actgccggaa gaaaaacgta aattgctggt gcaactctga aaggaaaagg ccgctcggaa
     1801 ggcggcctta acgattacag catcggcttg aggaagcgtg ccgtatgcga agcttcgcac
     1861 tccgcgacgg tttctggcgt accggagacg aggatctcgc cgccgccact gccgccttcc
     1921 ggccccaggt cgacaatcca gtcagcggtt ttaatcacgt cgagattgtg ctcaatcacc
     1981 acaatggtgt tgccctgatc gcgcagttta tgcagtacgt cgagcagttg ctgaatatcg
     2041 gcgaagtgca gaccggtggt cggctcgtcg agaatataca gcgtctgccc ggtgccgcgt
     2101 tttgacagct cacgcgccag cttcacgcgc tgggcttcac caccagaaag tgtggttgcg
     2161 gactgcccca agcgaatgta cgtcagacca acgtccatca acgtttgcag cttacgcgcc
     2221 agagctggca ccgcatcaaa gaactcacgc gcctcttcga tggtcatatc cagcacttcg
     2281 tggatggttt tgcctttgta cttaatctcc agcgtttcac ggttatagcg tttacctttg
     2341 cactggtcgc acggcacgta aatgtccggc aggaagtgca tctccacttt gatcacgcca
     2401 tcgccctgac aggcctcgca gcgtccgcca cggacgttaa agctgaaacg tcctggcgta
     2461 taaccacgcg cacgggattc cggtacgccc gcaaacagtt cgcgcacagg cgtaaacacg
     2521 ccggtatagg tcgccgggtt agaacgcgga gtacgaccaa ttgggctttg gtcgatatcg
     2581 attactttgt cgaaatgctc cagtccctga atatcgcgat acggtgccgg ttcggcgatg
     2641 gtcgcaccat tcaactggcg ttgggcaatc gggaacagtg tgtcgttaat cagcgtcgat
     2701 ttaccggaac ctgaaacccc tgtgatgcag gtaaacagac cgactggcag cgttagcgtc
     2761 acgtctttca ggttgttgcc gcgtgcgcca gtcagcttaa gtactttttc cggattcgcc
     2821 ggaacgcgtt tcttcggcac ttcaatcttg cgtttaccgc tcatgtactg tccggtcaac
     2881 gactccggca ccgccataat cgcttccagc ggaccttccg cgaccacttc accgccatgt
     2941 acacctgcac caggaccgat atcgatcaca tgatcagcgg cgcgaatcgc gtcttcgtcg
     3001 tgctccacca caatcacggt attaccgaga tcgcgcagat ggataagcgt acccaacagg
     3061 cgctcgttat cgcgctggtg caggccgata gacggctcat ccagcacgta cataacgcca
     3121 accaggcccg caccaatctg gctcgccaga cggatacgct gggcttcacc gccggaaagc
     3181 gtttctgccg agcgggaaag cgtcaggtaa ttcaggccga cgttaacgag gaatttcaaa
     3241 cgatcgccga tctctttaag aattttttcc gcaatcttcg cccgctgacc tgcgagtttg
     3301 agattgttga agaattccat cgcatgacca atgctcatgt cggagatggc gggcagcggc
     3361 gtattctcga catacacgtg gcgcgcttcc cgacgcagac gcgtcccttc gcagctggcg
     3421 cacgggcgat tgctgatgaa cttggctaat tcttcacgta ccgcactgga ttctgtctct
     3481 ttataacggc gctccatatt gtgcagcacg ccttcgaacg gatgacgacg gatggaggta
     3541 tcgccacgat cgttcatgta tttgaattca atgttttctt tgccagaacc gtacaacacc
     3601 actttatgca cgttcgcgct caggctgccc cacggcgctt cgacgtcgaa cttatagtga
     3661 tctgccagcg atttcagcat ctggaaataa tagaagttgc ggcgatccca gccacggatc
     3721 gcaccacctg ccagcgacag ttccggattc tggatcacgc ggtcaggatc gaaatattgc
     3781 tgtacgccaa ggccgtcgca ggtcgggcag gcacctgccg ggttgttaaa cgaaaacagt
     3841 cgcggctcca gttcacgcat actgtagccg caaattgggc aggcgaagtt ggcggaaaac
     3901 agcagctctt ccgctttcgg gtcgtccata tccgccacta ccgcggtacc accggaaagc
     3961 tccagcgcgg tttcaaacga ctcggcaaga cgttgggtaa gatcgtcacg caccttgaag
     4021 cgatcaacca ccacttcaat agtgtgtttc ttttgcagtt ccagtttcgg cggatcggaa
     4081 agatcgcaga cttcgccatc aatacgagca cggatgtaac cctggcttgc caggttctcc
     4141 agcgttttgg tgtgttcgcc tttgcgctct ttaatgattg gcgcgagcag catcagacgc
     4201 ttgccttccg gctgcgacag cacgttatcc accatctggc tgacggtttg cgccgccagc
     4261 gggacgtcgt ggtccggaca gcgcggttcg ccgacgcggg cgtacaacaa acgcaaatag
     4321 tcgtggattt cggtgattgt ccccaccgta gaacgcgggt tatgagacgt cgatttctgc
     4381 tcaattgaga tggcaggaga aagcccctca atatgatcga cgtccggctt ttccatcagt
     4441 gacagaaact gccgcgcgta ggcggaaagg gattcaacgt aacggcgctg cccttcggca
     4501 tataaggtgt cgaaagcgag cgaggatttg ccagaacccg aaagcccggt cacgacaatg
     4561 agcttgtcgc gggggataac gaggttgatg tttttgagat tatgggtgcg ggcgccccga
     4621 acttcgatct tatccattca cctttcccgg attaaacgct tttttgcccg gtggcatggt
     4681 gctaccggcg atcacaaacg gttaattatg acacaaattg acctgaatga atatacagta
     4741 ttggaatgca ttacccggag tgttgtgtaa caatgtctgg ccaggtttgt ttcccggaac
     4801 cgaggtcaca acatagtaaa agcgctattg gtaatggtac aatcgcgcgt ttacacttat
     4861 tcagaacgat tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat
     4921 tctcgttggt aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt
     4981 tgccaacatt acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa
     5041 agagcagact gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggccagcga
     5101 atatctgcgt aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac
     5161 cgatcaatcc ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat
     5221 gcagatgctg ggtggtcgtc agggtggtgg cgctcctgca ggtggcaata tcggtggtgg
     5281 tcagccgcag ggcggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg
     5341 cggcgcgcag tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat
     5401 ggactttgat gacgatattc cgttctgatt tgtcattaaa acaataggtt atattgtttt
     5461 aaggtggatg attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc
     5521 tacgtatcag gtcaaaatca ttggccactg tggcggtgtc tgattcatct cttctatcat
     5581 caccgactcg atgttgtgcc agatggcggt gatgtccgcc attgtgatgc caagcaaccc
     5641 cattgcgaac cagcaggcgg aaaccacgcc cagaccgctg ctgatcactg caagcccgac
     5701 ataatcagac cgacgaaaac ggatgttgag cgtgctggcc agaaacatca ttacggcact
     5761 aagaagttgc cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct
     5821 ctgaaaagag ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct
     5881 ggctatcgcg gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg
     5941 tgaactatat cacaattgat tgtttgttag ccagattagg tcgtgacttt tattgctgta
     6001 cagattatgt ggtttttcag tggcattaag ggtatatctt cccgccgcct ctgtattcct
     6061 gtagaaaatt aattttgaat atcaatgaat tattttcatc caagtgacga ttaaaaaggt
     6121 atcaatttca aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg
     6181 tagccagggt ttcaccttgc aatggccggg tataaacagg caggaaattg atagcaatga
     6241 atcatcgtgc acggcaccaa ttactggcgt tgccgggcat tatcttttta gttctctttc
     6301 ccatcattct ttcgctatgg attgccttcc tttgggcaaa atcagaagtg aataatcagc
     6361 tccgaacctt tgctcaactg gcgttggata aatccgagct ggtcattcgc caggcagatt
     6421 tagtgagcga tgcagctgaa cgctatcagg ggcaagtttg cactccagcc catcaaaagc
     6481 gaatgttgaa tattattcgt ggctatcttt atattaatga attgatctat gcccgtgata
     6541 atcatttttt atgctcatcg ctgatagtgc ctgtaaacgg ctatacgatt gtatcggccg
     6601 attataagcg tgaacctaat gtttctatct attattaccg cgatacgcct tttttctctg
     6661 gctataaaat gacctatatg cagcggggaa attatgtggc ggttatcaac cctctcttct
     6721 ggagtgaagt gatgtctgat gacccgacat tgcaatgggg tgtgtatgat acggtgacga
     6781 aaaccttttt ctcgttaagc aaagaggcct cagcagcaac gttttctccg ctgattcatt
     6841 tgaaggattt aaccgtacca agaaatggct atttatatgc gacagtttat tcgacaaaac
     6901 gcccaattgc agccattgtt gcgacttcat atcaacgtct tatagcccat ttttataatc
     6961 atcttatttt tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc
     7021 tacgtattcg acaaaactat ttgtctccca aacgcaaatt gcaacgcgcc ctcgaaaaac
     7081 atcagctatg cctttattac cagccaataa ttgatatcaa aacagaaaaa tgtatcggcg
     7141 ctgaagcgtt gttacgttgg cctggtgagc aggggcaagt aatgaatccg gcagagttta
     7201 ttcctctggc agaaaaggag gggatgatag aacaggtaac tgattatgtt attgataatg
     7261 tcttccgcga tctgggcgcg taccttgcaa cacatgctga tcgctatgtt tctattaatc
     7321 tgtcggcctc cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc
     7381 aatacgcggt gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg
     7441 tcgacaaaat gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg
     7501 atgattttgg tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt
     7561 tgaaaatcga caaatcgttt gttgagacgc tgaccaccca taaaaccagt catttgattg
     7621 ctgaacatat catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg
     7681 aaactgagga gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt
     7741 tctttgcgaa ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc
     7801 gggagttaac gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat
     7861 caaactgccg acggaaaacg cgggagaagg tctgctgaga gacataaccc aggtccattg
     7921 cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacagacggc
     7981 gttgacgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatc cgttgcaggt
     8041 accactttga atagcctgat ttttttgcga cgacatcaat gttaagcggc tggtcaatat
     8101 gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc
     8161 ttttcagtgt tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg
     8221 aggtaaagcg atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg
     8281 aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa agcaaagggt
     8341 tgattaccag tatccgtaac agcggcaatc agcggcgtta taaacgtgat gtgttgcgat
     8401 atgtcgcaat tatcaaaatt gctcagcgta ttggcattcc tctcgcgacc attggtgaag
     8461 cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt
     8521 cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac
     8581 tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg
     8641 gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa
     8701 gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt
     8761 acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg
     8821 gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac
     8881 tttttagccg caatattttt gtcatctcag acgatttttt atcgcaatcc tcaacggtat
     8941 acggctcgat aacgctgcaa tcttgcgcat cgacgataac gtttgcgcat caattgcctg
     9001 gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg
     9061 ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa
     9121 acgtttgctt ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa gataacgttt
     9181 cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttc
     9241 tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc
     9301 acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat
     9361 ggtctactcg gttatcgtcg ttccaggcat gttgggtaaa gcgggcttcc cgcctgcggc
     9421 agttttcgtt gcgacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc
     9481 taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct
     9541 ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt
     9601 gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgccgca
     9661 cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca ttgccgctaa
     9721 cggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt
     9781 cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa
     9841 actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat
     9901 cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga gcgatgaaaa
     9961 cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc
    10021 aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta tccgcgctgt
    10081 cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggcg ggaaagcact
    10141 gaccactgac tccatgagca gcgttttctc tggcttggtg ggtgcagctc cggcagcggt
    10201 atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac
    10261 cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctttcttacc tcgttccggg
    10321 gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa
    10381 aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt
    10441 gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg
    10501 tctggtttcc ggtgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct
    10561 ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt
    10621 ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga
    10681 aaaaatgaga attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga
    10741 acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt
    10801 ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc
    10861 gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt
    10921 cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct
    10981 cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg
    11041 cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct
    11101 ggcggcggtg ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg
    11161 catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg
    11221 cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg
    11281 cggtgcgacg gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt
    11341 gagctggctg tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc
    11401 gacgcagatc gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat
    11461 tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt
    11521 gatgcgccgt gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga
    11581 atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga
    11641 gacgtcgctg atggcagcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga
    11701 tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa
    11761 aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg
    11821 agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactttgg ccggtgtgct
    11881 ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt
    11941 tctggcggct ggtgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct
    12001 gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac gtattgcgcg
    12061 agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc
    12121 ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg
    12181 taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa
    12241 ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct
    12301 gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt
    12361 gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga
    12421 ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc
    12481 atgattctaa aacaaataag ttatctctct tttaaagtca tttcattggc tgttcttcga
    12541 cttaatctca atctgcgtgt tgcataatat ctgcagtaat cttcatagct acttcatgag
    12601 tattattctc agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct
    12661 gtagtgttac cagggtatca ctattaatgg gaatattctc ctccccacgg agaatggaaa
    12721 tgcccattcc ctggacgcta gcctcatcga aattaacccg atagagacgg gtattaaaca
    12781 tccaggcttt tgttaaaaca gaacagttga aatggcaatt gtttaaattg gcat
//
LOCUS       DAZFKY010000137        12834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFKY010000137 DAZFKY010000000
VERSION     DAZFKY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(127..591)
                     /gene="nlpC"
                     /locus_tag="ACHKCL_003449"
     CDS             complement(127..591)
                     /gene="nlpC"
                     /locus_tag="ACHKCL_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5764342.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(669..1418)
                     /gene="btuD"
                     /locus_tag="ACHKCL_003450"
     CDS             complement(669..1418)
                     /gene="btuD"
                     /locus_tag="ACHKCL_003450"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5764343.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1418..1969)
                     /gene="btuE"
                     /locus_tag="ACHKCL_003451"
     CDS             complement(1418..1969)
                     /gene="btuE"
                     /locus_tag="ACHKCL_003451"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5764344.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(2032..3012)
                     /gene="btuC"
                     /locus_tag="ACHKCL_003452"
     CDS             complement(2032..3012)
                     /gene="btuC"
                     /locus_tag="ACHKCL_003452"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5764345.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3113..3412)
                     /gene="ihfA"
                     /locus_tag="ACHKCL_003453"
     CDS             complement(3113..3412)
                     /gene="ihfA"
                     /locus_tag="ACHKCL_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5764346.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3417..5804)
                     /gene="pheT"
                     /locus_tag="ACHKCL_003454"
     CDS             complement(3417..5804)
                     /gene="pheT"
                     /locus_tag="ACHKCL_003454"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5764347.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5819..6802)
                     /gene="pheS"
                     /locus_tag="ACHKCL_003455"
     CDS             complement(5819..6802)
                     /gene="pheS"
                     /locus_tag="ACHKCL_003455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5764348.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(7011..7135)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(7085..7129)
                     /gene="pheM"
                     /locus_tag="ACHKCL_003456"
     CDS             complement(7085..7129)
                     /gene="pheM"
                     /locus_tag="ACHKCL_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5764349.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(7252..7608)
                     /gene="rplT"
                     /locus_tag="ACHKCL_003457"
     CDS             complement(7252..7608)
                     /gene="rplT"
                     /locus_tag="ACHKCL_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5764350.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7661..7858)
                     /gene="rpmI"
                     /locus_tag="ACHKCL_003458"
     CDS             complement(7661..7858)
                     /gene="rpmI"
                     /locus_tag="ACHKCL_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5764351.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7955..8497)
                     /gene="infC"
                     /locus_tag="ACHKCL_003459"
     CDS             complement(7955..8497)
                     /gene="infC"
                     /locus_tag="ACHKCL_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5764352.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8501..10429)
                     /gene="thrS"
                     /locus_tag="ACHKCL_003460"
     CDS             complement(8501..10429)
                     /gene="thrS"
                     /locus_tag="ACHKCL_003460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5764353.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10952..>12834
                     /gene="espL1"
                     /locus_tag="ACHKCL_003461"
     CDS             10952..>12834
                     /gene="espL1"
                     /locus_tag="ACHKCL_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO5764354.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADE"
BASE COUNT         3253 a         3368 c         2916 g         3297 t
ORIGIN      
        1 gtcctttttt tagattatcc tgattataaa cgaataatcc atttacggaa tttttgtctg
       61 caaaatacta ctgtattctg gagtaaatgg acggcgatgt atcatcgacg tccaattttc
      121 gacatactag attcgtctcg cctgccagaa atttttctgc caatagacat tatcaagtga
      181 ggaacgcatc actcccttgc tggtagaggc gtggataaat tggttgttgg tatcgtaaat
      241 acctacatgc aaaccatttt gtccggaacc cgttttgaaa aagaccaggt caccaggcag
      301 caactcgtct ttatcaattt gcgtgccgat agaggcttgt tctttggttt ctcggggcag
      361 ctgcaaatcg aaacgatcgc gcatcgtcac aaccacaaat cccgaacagt ccacaccgcg
      421 ccgcgtcatg ccaccataac gatacggcgt gccatgccag ctttgtagct ggtcgttcaa
      481 accggcaata acggtaatcg aatcagaaag tctggcattt ggcggcggtg ctttatggtg
      541 gctacacccg gccagaaaca gtgctgtgat caaaataagg cagaaacgca ttccgtacgg
      601 ttcctctgtt ttttattctt gcattaattt agcgtcgtaa ttacccgatt ttcaagatac
      661 taatgaaatc agatggtcga aatcagcatt ctgtgacctt cgatatccag acggcgaaaa
      721 ttcatcccat aggcctgcgc cagatttggc ggcgtgagca catcttctct gcgtccactg
      781 gccagcattt ttccaccttt tagcaaccac gcccgatgcg catgacgcaa tgtgtggttg
      841 agatcgtgac tgctcatcac aatcgccagt ccttgctgac acagcgcgct cagaattttg
      901 tctaacgcac tttgttgcgc aacatcaaga ctgttcatcg gctcatcaag aagcagcaat
      961 tggcctgcgg gattggcttg tggtgtgatt tgcagcacca ccgcagcaag acgtacgcgt
     1021 tgccattcac cgccggaaag ttgattggtg ctacgaccga gtttgtcatc aagagccagc
     1081 gcccctgcga catcattcag tagttcggta cgcgttttat cgtgctgatg cagtgtcagg
     1141 tagtgccaga ccggcattgc aaacggcggc gtctgctgtt gtgaaagata ggcacgatgc
     1201 agcgcgagtt ttgttgcgga ccatgcttcc agtggttgcc ccgcgaactg aatgcttccc
     1261 ttaccgctgg tcattccggc cattcgcgcc agtaaggtac tcttacccgc gccattcggc
     1321 cccaccaggt gcaggatctc cccagcccga acctcgccag aaagcggccc caggcgggta
     1381 gattccgcaa catcctgtaa ctgcatcaca atagacatta ttttgccaac gccagtttaa
     1441 tgctttccat cacaatggga tcttccggcg tcatatccgg ggaaaaacgc tggatgacca
     1501 atccgtccct gccaactaaa aatttctcaa aattccataa aatatcatca gggtacagcg
     1561 gtgcacgacc tttgctgacc atacgggcat agaatccgct ctcttccggc gcgactgcgg
     1621 tcggcgctgc ggaaatcaat ttttgataca gcggatggcg tccttcgcca ttaacttcaa
     1681 tcttactgaa catcgggaac gtcacccccc atgtggtggt acagtaagtt ttaatctctt
     1741 catcgctgcc cggttcttgt tccagaaact ggttgcacgg gaatcccagc accacaaaac
     1801 ctcgatcggc ccaggctttc tgaatattct ccaactgctc atattgcggc gttaagccac
     1861 actttgaggc gacattgaca atcaacagca cattaccggc gtacttctcc agcgtggtca
     1921 cttcaccgtc gatatctttc actacggtcg tcagaatgga atcttgcatc gtttctcctg
     1981 ggtgtggtca gtaaaaatct tagcttttaa tcatagaccg tctttttgcg gctaacgtcc
     2041 tgcttttaac aataaccaga taaacaccgg cgcacccaac gttgcggtga ccacgccaat
     2101 aggcagctct gcggcagcta atgccaggcg cgctacaata tcggccagca gcaatgcgct
     2161 cgcccctgcc agcgcgcagc cgggaagtaa tgcgcgatga tcggttaaac cacacaaccg
     2221 aagaatatgt gggatcacca gaccaataaa gccgatagca cccgccagcg ctacactgac
     2281 gccaaccatc cagccggtcg ctgccaccag cacattgcgc cagaaccaca ggggtaaacc
     2341 cagttgccgc gccgagatct cgccaagtgc taacatattc atcggcctgg actgacaaca
     2401 gatccacaac aacatgggga tcaatgccag catcagccag ctttgccgcc agtctacgcc
     2461 gccaaaaccg cccatcatcc agtacatcag ctgacgtaaa tcaacagagg tggaaaagta
     2521 gatagcccac gtcattagtg cgctacagat aatccctaat gcaacgccag ccagcaataa
     2581 ccgactggtc gaaagatgac gacgggcgaa acgtaagagt attaaagtga tgataagcgc
     2641 cccagcaatc gcacacagcc ctagcgccca gttggggagt tgcccttgcc caagcaatac
     2701 cgcggcgata agccccacgc ctgcgccgtt agagacgcca agtagtccag gttctgccag
     2761 aggattttca aacaacgcct gcattacagc gccggatata gccagcgccg caccaaccag
     2821 caatacagcc agcgtacgtg gcaggcgaat ttgccagacg aacagttcgc cacgaggaga
     2881 aaaccagtca cctggcgaga tccattgttc accggcgcaa aggcttaaga gaagcgccag
     2941 cagcatcaaa actgacaggc ataataacca gcgaatattt tgtcgctgtt gttggcgggc
     3001 aagtgtcagc atggtatccg ttctgctgaa gtgtcatggc gttgatttta cggtgactct
     3061 tcgacagtga aaagaaaaaa ggccgcagag cggccttttt agttagatca gattactcgt
     3121 ctttgggcga agcgttttcg acccggcttt ttaacttctg cccgggtctg aaggtcacca
     3181 cgcgccgtgc tgtaatggga atatcctcgc ccgttttcgg gttacgtccc gggcgttgat
     3241 tcttatcacg cagatcgaag ttaccaaaac cagagagttt cacctgttcg ccgttttcca
     3301 gagcgcgacg gatctcttcg aaaaacagtt caaccagttc tttggcatcc cgcttgctaa
     3361 gcccaagctt atcaaacaga tattctgaca tttcagcttt tgtaagcgcc ataggttcaa
     3421 tccctcaatg atgcctggaa tcgctctttt aatgcctcta cacatttggc gacggtagcg
     3481 gcaatctcct cttcttcgag tgtacggctg gtatcttgca ggatcaggct tatggcgagg
     3541 ctcttatacc cctccgcaac acccttaccg cggtacacgt caaataagtt tacgccaact
     3601 acctgattta cgccaacttt cttacattcg gataaaatat ccgctgcggg aacgttttct
     3661 gcgaccacca ccgcgatgtc acgacggttc gccgggaagc gagaaatctc gcgcgcctga
     3721 ggcaccacgc ggtctgcgag cttgttccac tccagttcga acaccagagt gcgaccgtta
     3781 agatccagtt tacgttccag ttcaggatga acaaccccaa caaaaccaat acgttcacct
     3841 ttcagataaa tcgctgcgga ttgccccgga tgcagtgccg gattcgcttc tgcacggaac
     3901 tcaacctcat tcagtttacc ggtcaggtcg agaacggatt caagatcgcc tttcaaatca
     3961 tagaaatcaa cggtctcttt tgccaggttc cagtgctctt cgtaacggtt accgcaaatc
     4021 acaccggcta acatcagatc ctgacgaatg cccaacggtg cctgagtatc tggtacgaaa
     4081 cgcagaccgc tttcgaaaat gcgcacacgg ttctgctgac ggttctggtt gtacaccacg
     4141 gttgccagca ggccggtcca cagagaaaga cgcattgctg acatttcaac agagatcggg
     4201 cttggcagca gtaaggcttc aacgcctgga tggatcatct gctgcacttt cggatcaacg
     4261 aagctatagg tgatcacttc ctgatagcct ttatcgttga gcagcgtttt cacgcgcttg
     4321 agcgacaggt cagcttcacg gtgagtaccc ataatcaggc ttgcctgtac cggctcatcc
     4381 gggatgttgt tgtagccgta aacacgcgcg acttcttcga ccagatcttc ttcaatctcc
     4441 atgtcgaaac gccagctcgg cgcaactgcc tgccactcgt ctttgccttc ggtcacttcg
     4501 cagccgagac gacgcagaat gtcagttacc tgctcatccg caatatgatg gccgatcagg
     4561 cgatccagtt tgctacgacg taaagtgatg gttgcacgct tcggcagcgt tgcttcgttg
     4621 gtgatatcaa ttaccggacc agcctcacca ccgcagatgt caatcagcag acgggtcgca
     4681 cgttccatcg ctttgtactg cagtgccgga tcaacgccac gctcataacg gtgagacgca
     4741 tcagtatgca ggccatgacg acgagcacga ccggtgatag acagcgggct aaagaaagcg
     4801 cattccagca gcacgttttg tgtttcgtca ttcacgccag agtgttcgcc accgaagatg
     4861 ccgcccatcg ccagcgcctt gttgtggtcg gcgatgacca gagtgtcagc attcagcttc
     4921 gcttcagtac cgtcgagcag caccagcgtt tcgccctctt tcgccatccg caccacaatg
     4981 ccgccttcaa tgcgatcttt atcgaaagcg tgcatcggct ggcccaattc gagcagcaca
     5041 tagttggtga cgtcaacaac tgcatcgata gaacggatcc cgcaacgacg cagtttttct
     5101 ttcatccaca gcggagttgg cgctttaacg ttaatgcctt ttaccacacg gccaagataa
     5161 cgcgggcagg cttccggcgc ttcgactgta atcggcagcg tgtcgtcgat ggtcgcacca
     5221 accggaacga tttccggttc aaccagcggc agctgattca gcacggcaac gtcacgcgca
     5281 acaccaatga tacctaaaca gtcggcacgg tttggcgtta cgctgatttc gatggtgttg
     5341 tcatcgagtt tcaggtattc gcggatgtca gtgccaatcg gcgcatctgc aggcagttcg
     5401 ataatgccgt tatggtcgtc agaaattccc agctcagaaa aggagcacag catcccttca
     5461 gacggttcgc cgcgcagttt cgccgcttta attttgaaat cacccggcag aacagcacca
     5521 atggtcgcta ccgccacacg caggccctga cggcagtttg gcgcaccgca gacgatgtcc
     5581 agcaggcgat cgccgccgac attcactttt gtcacacgca gtttgtcagc gttcggatgc
     5641 tgcgcacact caaccacttc accaacgacc acgccgtgga agctaccggc aaccggttct
     5701 acaccgtcaa cttccaggcc cgccatagtg atttgattcg ccagcgcatc gctatcaatc
     5761 gccgggttca cccattcgcg taaccacagt tcactgaatt tcataatcta ttcctgcctt
     5821 atttaaactg tttgaggaaa cgcagatcgt tttcgaagaa tgaacgcagg tcggtgacgc
     5881 cgtaacgcaa catagtcaga cgctccatcc ccatcccgaa ggcgaaacca gagtaaactt
     5941 ccgggtcgat gccaacgtta cgcagcacgt tcggatgcac catcccgcag cccagtactt
     6001 ccagccattt accgttttta cccatgacat ccacttctgc agaaggttcg gtaaacggga
     6061 agtaggaagg acggaagcga atctgcaaat cttcctcaaa gaagttacgc aggaagtcgt
     6121 gcagcgtgcc tttcaggttg gtaaagctga tgttggtatc aacaatcaga ccttccatct
     6181 gatggaacat cggcgtgtga gtctggtcgt agtcgttacg ataaacacgg ccaggcgcga
     6241 tgatacgaat cggtggctgc tgggctttca tggtgcggat ctgtacgcca gaggtctggg
     6301 tacgcagcag gcgggtagcg tcaaaccaga aagtgtcgtg gtcagcgcgc gccgggtggt
     6361 gaccaggaat gttcagagca tcgaagttat gataatcgtc ttcgatttcc ggcccggttg
     6421 ccacggtaaa gccaagctca ccgaagaaac tttcgatacg gtcgatggta cgggtaaccg
     6481 gatgcagacc gccgttttca atgcgacgac ctggcagaga gacatcaatc gtttccgccg
     6541 ccagacgcgc attcagtgca gcgctttcca gttccgcttt acgcgcattc agcgcctgct
     6601 gaacctgctc tttcgcttcg ttgataaccg caccggctgc cggacgctct tctggcggca
     6661 gctcacgcag ggtcgtcatc tgaagggtta agtgcccttt tttacccaaa tattcgacgc
     6721 gcacattgtc taacgcggca acatctgacg cctggctaat ggccgccttc gcactggcaa
     6781 ccagttctgc gagatgtgac atggttttcc tcattgtgtc agtggtgaca ctggttcgtt
     6841 ggacttagag cctatcccat caggctattt tacttgccat tttggtcccg ggcagtgctc
     6901 gaaatcctca cgtacttaag tacgctccgg tttctccgca ctggccgtat ccagactggc
     6961 tgcgacaatt acgcctgata gactggcttt ttatttttca aaacgcgcat acaaaaaaag
     7021 cctccactgg gaggctttca ggcgctgttt tccgtttctc ttctcacgcg ctagcctcct
     7081 ggattcaggt gctaaagtaa aaaaagaagc ggaaaatagc agcattcatt gcttgcgtta
     7141 ccttttggta ctcttcaaaa gacctttatt gaaaaggcta cggcgataaa agtcaatgtt
     7201 ttgatggcgt tgaaacgaaa agagggagac tagctccctc tttcaactgg cttatgccag
     7261 agctgctttc gctttttcaa ccagagcggt gaacgctact ttgtcgaata ctgcgatatc
     7321 agccaggatc ttacggtcga tttcaacaga ggcttttttc aggccattga tgaatttgct
     7381 gtaagaaata ccgttctgac gtgctgctgc gttgatacgc gcaatccaca gttgacggaa
     7441 ctgacgctta cgttgacgac ggtcacggta agcatactga ccagctttga taacagcctg
     7501 gaaggcaacg cggtatacgc gagaacgcgc accgtagtag cctttagctt gtttcaaaat
     7561 tttcttgtga cgtgcgcgtg caataacacc acgttttacg cgagccatat gtgctctcct
     7621 gtatctatat tctaattaaa aagttaaaaa cgttaacggc ttatgcgtac ggcaggcacg
     7681 cgattaccag gcccagatcg cctttggaaa ccatggcttt cggacgcagg tgacgtttac
     7741 gtttggtcgc ttttttggtc agaatgtgac gcaggttagc gtgcttgtgc ttaaaaccac
     7801 ctttaccggt ttttttgaag cgcttagcag caccgcgtac ggtcttaatt tttggcattt
     7861 taataacttc cacttcgcat tgttaataaa cgaaacaaag gcgaacaaag cctgtgaagc
     7921 ccgaaggctc cacagacagt gctacttgaa ggccttactg tttcttctta ggagcgagca
     7981 ccatgatcat ctggcggcct tcgatcttcg ttgggaagga ttcgaccact gccagttctt
     8041 gcaaatcgtc tttcacgcga ttaagcactt ccataccgat ttgctggtgc gccatctcac
     8101 gaccgcggaa acgcagcgtg attttggctt tatcaccctc ttcgagaaag cgaatcaggc
     8161 tgcggagttt tacctgatag tcgccttcat ctgtaccagg acggaattta atttccttaa
     8221 cctggataac tttttgcttt ttcttctgtt ccttagaaga cttgctcttt tcatagagga
     8281 atttgccgta atccattata cgacaaaccg gcggctcggc gttagggctg atctcgacta
     8341 agtctactcc ggcttcttct gctttctcca gagcttctct cagactcaca ataccaagct
     8401 gctcgccttc cagacctgtt aagcgaactt cctgggcgcg aatttcgcca ttgatacggt
     8461 tagggcgcgc cgtttgaact cgttttccgc ctttaatacc ttattcctcc aattgtttaa
     8521 gactgcggct gcgaatctct tgttgcagct tctcgatcac ttcatttacg tccatgcttc
     8581 ccaggtcttt accacggcgg gtgcgaacgg caattttgcc tgattccacc tctttatcac
     8641 cacagaccag catatacggg acgcgacgca aagtgtgctc gcggatttta aagccaatct
     8701 tctcatttct caagtctgct ttaacacgaa tgcccgcatt tgatagtttt tgcgtcaatt
     8761 cgttaacgta ttcagactgt gaatcggtaa tattcatgat aacaacctga actggcgcaa
     8821 gccaggtcgg gaagaaacca gcgaattctt cggtcaggat accgatgaaa cgttccatcg
     8881 accccagaat tgcgcggtga atcattaccg gtaccttacg ctcgttgtct tcgcccacat
     8941 aggaggcgct cagacgagac ggcaaggaga agtccagctg tactgtaccg cactgccatg
     9001 cacgatcgag gcagtcatac agggtaaatt caattttcgg accgtagaaa gcgccttcac
     9061 ccagttgata ttcaaacggg atgttgtttt cttccagcgc aaccgccagg tccgcctcag
     9121 cacgatccca catttcgtcg ctgccaatac gcttttcagg acgagtggag agtttgacga
     9181 cgatcttctc gaagccaaaa gtgctgtaca tatcatagac taaacggata catccgttaa
     9241 cttcatcgcg aatttgttct tcagtacaga agatatgcgc gtcatcctgg gtgaatccac
     9301 gcacgcgcat caggccatgc agagaacctg acggctcgtt acggtggcag ctaccaaact
     9361 cggccatacg cagcggcaga tcgcgataag acttcagccc ctggttgaaa atttgtacgt
     9421 gacccgggca gttcatcggc ttaatgcagt attcacggtt ctcagaagac gtggtgaaca
     9481 ttgcatcttt gtagttgtcc cagtgaccgg ttttttccca caggacacgg tccatcatga
     9541 acggaccttt aacttcctga tactggtact ctttcagttt agaacgaaca aacacttcca
     9601 gttcacggaa gatggtccag ccgtcgttgt gccagaatac catacccggc gcttcttcct
     9661 gcatatggta caggtcgagc tgtttaccga ttttacggtg gtcgcgtttc gcggcttctt
     9721 ccaggcgctg caggtaagcg ttaagcgctt ttttgtctgc ccacgccgta ccgtaaatac
     9781 gttgcaacat tttgttgttg ctgtcgccac gccagtaagc ccctgccgtt ttcattagtt
     9841 tgaaatgatg gcagaaacgc atgttcggta cgtgcggacc gcggcacata tcgacatatt
     9901 cttcatggaa gtacagacct ggcttgtcat catgggcgat gttttcgtca agaatggaga
     9961 ctttgtagct ctccccacgg ttggcgaaag tttcacgcgc ttcgtgccag ctgactttct
    10021 tcttaatgac gtcgtagttt ttctcagcaa gctcatgcat ccgcttctcg agtgcttcga
    10081 catcttcctg ggttaacgtg cggtcaagat caacgtcgta ataaaaaccg ttgtcaataa
    10141 ccgggccgat tgccattttg gtatgcggcc aaagttgttt aatcgcgtgc cctaacaggt
    10201 gcgcacagga gtgacgaatg atctccagac cttcttcgtc tttggcggta atgatcgaca
    10261 gttgtgcgtc gttttcaatc agatcgcaag catcaaccag ttcgccatta acgcgccctg
    10321 cgatacaggc tttcgccaga cctggaccaa tgtccagcgc aacatccatg gggcttacag
    10381 cgtggtcgta atggcgttga ctgccatcag gaagagttat aacaggcatt ttatatcctt
    10441 atttgcagtg gtgacccaca cgaaagatca catacaaaga aaaatttgtt tattaacagt
    10501 tcattgcgaa accatctagc caacaaatgc taaattggtt cgcaatcagg tacacaaatg
    10561 aaagacatta acctctcacc ttccacccgg ttgagcgatg ttaacactaa aaaaagggag
    10621 attgtacctt tccgtttcac attgattcga ttcgcaattc gtttgtagtg agtacaccag
    10681 caaaacgctt aaagaacaac tgataacatc acggagcgtc tgaaaggttt actgcgagcg
    10741 aatttacagc agcataaact gaatccagca aagttcgttg cgtgatttgg cggtactatt
    10801 taaacgatca taagattaat gacgaagtgg tcatatcaca atgataaaag tgacacaatt
    10861 cttatagcaa tttttcgtgc acatttcgtt ctggcgataa taattaatca tcatactcaa
    10921 aacctccaga catatataag gcgaatagat tatgagtcaa aacgacatta ttatcagaac
    10981 tcattataag tctcctcata gaatgcacat cgatagcgac ataccaacgc cttcatcaga
    11041 gcctattaat caatttgcgc cccaactcat caccctactt gatacctctg acttaagttc
    11101 gatgctgtca tactgtgtta ctcaggaatt taccgcaaac tgtcgaaaaa tatcacaaaa
    11161 ttgttattcc actgcccttt ttaccattaa ctttgccact tcacccatcc atgcagaaaa
    11221 tatattcatt acattacact ataaaaaaga aatcatttcc ttattactgg aaaccacgcc
    11281 tattaaagct aaccatttgc gaagcatact ggattatatt gaacaggaac agttaactgc
    11341 cgaaaatcgt aaccattgta tgaaactgtc taaaaaaatc catagagaaa aaactataca
    11401 accaacagta aatctcaatg gtagtgcatt tttttcgcaa tctccttctg acgctatttt
    11461 ttgtcgccat ctgtcattgc aatacgcact tgattcattg agaaatggaa aaggcaaggt
    11521 caacctgatt aaacattact cctccgttga atccatacag cagcatgtcc ccttagtccg
    11581 ggacgcggag ttcagagcat tacttcgcca tcctcctgca gggagtcgcg ttatcgcgag
    11641 taaggatttt ggcttcgctt tagatatttt cttctgtcga atgatggcaa acaatgtcag
    11701 tcatatgtct gcgattttat atatagacaa tcatactttg tcagtaaggc tacgaataaa
    11761 gcagtcggcg tatgggcaat taaattatgt tgtgtccgtt tacgacccga acgataccaa
    11821 cgttgccgtc agaggcaccc acaggacagc acggggcttt ctctcgcttg ataagttcat
    11881 cagttcaggt cccgatgctc agacctgggc tgataggtat gttcgcaact gtgcaattgc
    11941 tattctgccc ctattacctg agggagttcc aggggctatt ttcacgggta tcgcgacacg
    12001 aatgccattt gcccctatac atccatcggc aatgttgtta ataatggcca caggccagac
    12061 tcaacagctt attacattat tcagacagtt gcccatactc cctgaaaaag aaatcattga
    12121 aataataact gcgcagaata gcattggtac acctgcttta tttttggcta tgatgaacgg
    12181 acatactgac aacgtgaaaa tatttatgca agaaattcag tcactggtag ataatcacat
    12241 cattcatgaa gataatctgg ttaaattgtt gcaaactaaa agtgctaacg aaacacctgg
    12301 actttatatc tccatgttgt atggattcga tgagataatc gatatctttc tgaatgcatt
    12361 aaccactcct atagcacaag agcttttaaa caaaaaactg gtgatgagta ttttagccat
    12421 gaaaatacat gatggtgagc caggattata cgccgcaatg gaaaataatc accctttgtg
    12481 tgtcacacgg ttcctctcta aaattaatgg catcgccttt aaatacaagt tgagcaaagc
    12541 taacatcatg gatttattaa aaggcgctac agcacaggga acccctgcat tatacatcgc
    12601 tatgagcaag ggtaatgaag acgtcgtgtt atcttatata tcgacgctgg gtgcttttgc
    12661 aaaaaaacat tcttttagtc aacatcagtt atttacactg ttggctgcta aaaatcatga
    12721 caacatgtca gctgttcata tagccattca tcataatcat tataaaactg tagaaacata
    12781 ttatgctgct ataaatgtaa tcagccaaag catgagtttt agtgctgatg aatt
//
LOCUS       DAZFKY010000138        12664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFKY010000138 DAZFKY010000000
VERSION     DAZFKY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(2..721)
                     /gene="dcuR"
                     /locus_tag="ACHKCL_003462"
     CDS             complement(2..721)
                     /gene="dcuR"
                     /locus_tag="ACHKCL_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5764355.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(718..2349)
                     /locus_tag="ACHKCL_003463"
     CDS             complement(718..2349)
                     /locus_tag="ACHKCL_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5764356.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2530..2760
                     /gene="yjdI"
                     /locus_tag="ACHKCL_003464"
     CDS             2530..2760
                     /gene="yjdI"
                     /locus_tag="ACHKCL_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5764357.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2772..3044
                     /locus_tag="ACHKCL_003465"
     CDS             2772..3044
                     /locus_tag="ACHKCL_003465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5764358.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3271..3567
                     /gene="ghoS"
                     /locus_tag="ACHKCL_003466"
     CDS             3271..3567
                     /gene="ghoS"
                     /locus_tag="ACHKCL_003466"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5764359.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3595..3768
                     /gene="ghoT"
                     /locus_tag="ACHKCL_003467"
     CDS             3595..3768
                     /gene="ghoT"
                     /locus_tag="ACHKCL_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5764360.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3887..5404)
                     /gene="lysS"
                     /locus_tag="ACHKCL_003468"
     CDS             complement(3887..5404)
                     /gene="lysS"
                     /locus_tag="ACHKCL_003468"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5764361.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5641..7098)
                     /gene="dtpC"
                     /locus_tag="ACHKCL_003469"
     CDS             complement(5641..7098)
                     /gene="dtpC"
                     /locus_tag="ACHKCL_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5764362.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(7157..9304)
                     /gene="cadA"
                     /locus_tag="ACHKCL_003470"
     CDS             complement(7157..9304)
                     /gene="cadA"
                     /locus_tag="ACHKCL_003470"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5764363.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9384..10718)
                     /gene="cadB"
                     /locus_tag="ACHKCL_003471"
     CDS             complement(9384..10718)
                     /gene="cadB"
                     /locus_tag="ACHKCL_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5764364.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11084..12622)
                     /gene="cadC"
                     /locus_tag="ACHKCL_003472"
     CDS             complement(11084..12622)
                     /gene="cadC"
                     /locus_tag="ACHKCL_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5764365.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         3482 a         2942 c         2954 g         3286 t
ORIGIN      
        1 gttattggca atattgtttc agtagtgagt agtgttctgc ctgaatacgg taacggtaaa
       61 ctggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag ttgaccagcc
      121 agataaggta tttacggcag gaaacacgcg aaatgttaac ctcgttggct agctcgtcgg
      181 ttgaaaactc atagtcctga tgcgcgtcaa tccactggca cagtgtgcgt aacgtctgcg
      241 gcgttaagcc ttttggcaag cgacgaggat cctgctcgtt ggagctgctg ccgtggatta
      301 gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt ttcttttgcc
      361 gccagccggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc aggtaatcca
      421 cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag gagatgacaa
      481 tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc ccgttctctt
      541 tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg aagataatct
      601 ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg atttgtgcta
      661 cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat acattgatca
      721 tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga aaatcccggg
      781 ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga caagtgctaa
      841 accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt gatcgatttt
      901 gtcgggtgcg atccccggtc catcatcgtt aacttcgcag tgcagccagc cgtgacggta
      961 gtgcaatgtt acgctaattt cgccaccggg ttccggccct aatgcttcca gcgcgttttc
     1021 tatcagattt cccaatgtgg taatcagcgt cgcgacctgg tcctcactgc cgctgtctgg
     1081 cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc ggttaatctt
     1141 gctgattaaa aaaccagcga taaccggaga tttgatctta cccagcagag agccaatctc
     1201 ttcctgatag ttattggctg ttttgagaat gtaatcttcc aactgcttat aactcttcag
     1261 atgcaataat ccgagaatca catgcaattt attcataaat tcgtgggatc gttcacgaag
     1321 tgcatcagca tagttgacca gaccgtcgag tcgctgcatc agtttacgaa cttcagtttt
     1381 gtccctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg gaacggtgtt
     1441 gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcggcg taccgtcgcg
     1501 taacacttcc gagacatcta ccacctgtga ccatgagtgg ctgagcgtcg acagtttctc
     1561 atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac
     1621 ctcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca tggcctggcg
     1681 ttgctcaaac agcgtggaga tttcgtaggg ttccaggccg aaaaggattt ttttcagtac
     1741 cttaaccaga atgcaggtgc caatcagtcc gaccagcatg ccaaataata ccgaccagat
     1801 aatgctccag cgactgtcat tgatctgttg ggtcacgcgg cttaactcaa ggccgatcgc
     1861 caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc gtaaagcctg
     1921 cgccagaaaa ccgcgattga tagcgacatt ttcttcgcca ttcagcgctt taaggatgtc
     1981 atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc gaagactttg
     2041 catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt ccgcgatggc
     2101 ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg gcgagtcggc
     2161 gagagtacgc gccactgcca gtgccttgtt ggctagcccg tctcgcgtca tatcactgat
     2221 ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg cgctgaccat
     2281 taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt agggcaatga
     2341 atgtctcatc agcttccttg tgtgacaaat ttcttaagca ttatctctga tgaggcgggt
     2401 aattcaaagg gagtaagaat gattggctat ataggggaag agactctggc aacggaaact
     2461 gccagtgctg tatgaagatt ccagggctat gcttatagtg aaaaccatac tgatgagaga
     2521 gggaaggtca tggatcaggc gctactggac gggggttatc gctgttatac cggcgaaaag
     2581 atcgatgtct atttcaacac tgcgatatgt cagcattctg gcaattgcgt acgtggcaac
     2641 ggcaagttat ttaatctcaa acgaaagccg tggatcatgc cggatgaagt cgacgttgcc
     2701 actgtggtta aagtgattga tacgtgcccg agtggtgcgc tgaaataccg tcataaataa
     2761 gcgagggtaa aatggaaata cgcgaaggcc acaataaatt ttacattaat gacgaacaag
     2821 gcaagcaaat cgctgaaatt gtctttgtgc cgaccggaga gaatttagcg attatcgaac
     2881 ataccgatgt cgatgaaagc ctgaaagggc aagggattgg taaacagctg gttgcgaaag
     2941 tcgtggaaaa aatgcgtcgg gaaaaacgaa aaattatccc attatgtcca tttgcgaaac
     3001 acgaatttga taaaacgcgg gagtatgatg atattcgcag ttgatggaag agtacagatt
     3061 cacgatattt ttcatgctct ccgcggtgtg atgcaggaga gcatctgaag ggtaggggga
     3121 tgcacaaaga atgggcagag agcgcgtttt tttgtcccaa gtcatcccct ttactgagca
     3181 aaaaaaagaa tatctcctat atgagaatca tcaatcgggg ttaataaatt ttgcgtcccc
     3241 agagtgttca atatttatag gagtcatatt atggaaggta aaaacaagtt caatacttat
     3301 gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg
     3361 atgtatgata tgaatttctc ttctatcgtg gctgatgaat atgggatacc acgacaattg
     3421 aatgaaaatt ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga agatttaatc
     3481 aggcttaaat gcttagactt accggatatt gactttgacc tcaacattat gacagttgat
     3541 gactatttcc gtcagtttta caagtagcca gtaaggataa gaagaacata aaaaatggca
     3601 ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc
     3661 tggtttatct cacatgagaa aacacatatt cgtttactta gtgcattcct ggtcggaata
     3721 acctggccaa tgagtctgcc tgtggcatta cttttttctc tcttttagga gctgtagttg
     3781 agtggacccg ctggttatcg tgaaacgaag cgtctctcct caaaactgga tttgcggaac
     3841 gatgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat ttctgtgggc
     3901 gcatcgccgg gaagagaata acgtcgcgaa tagtatggct gttagtaaac agcataatca
     3961 ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc agcgcagtca
     4021 cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc ggctttagcg ttaacctgtt
     4081 cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccgattt
     4141 cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca tcattacggc
     4201 gtgccagcgg tgacacttct gccggatatt ccgtaataaa ggttggctga atcagatgtg
     4261 cttctgccac ttcatcaaag atctccgtga caatacgtcc cagcccccag cttttctcta
     4321 ccgtaatacc gatagattca gctaatgctt tagcagcatc aaaattatcc aggtcggcca
     4381 tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt
     4441 tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccaga acctcttgtg
     4501 ccagggtgcg gaacagggac tctgtcagtt caatcaaatc gtggtaatcc gcatacgcca
     4561 tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga
     4621 agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca
     4681 gctccggcgc gatacgcaga tacatatcta aatccagagc attatgatgg gtaataaacg
     4741 ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc
     4801 cgcgcgcgac catgaactgg cggatagcgg ccagaatttt tgaacggaca acaaacgttt
     4861 gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat
     4921 cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc aggcgcaact
     4981 cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg cgggcaccga
     5041 taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat
     5101 ctcgtgcgac gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt
     5161 tccccatgat acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt
     5221 ccagttcctg gttatccttc gcatcaaact cttcgtgcaa ctggtcagag gtatggtcgc
     5281 ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc
     5341 gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tcttgttcag
     5401 acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta
     5461 cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta
     5521 cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact tattaatctc
     5581 gatttactag tgtgataaac tgaaagttaa tttattcata accaattgtt tttacgacag
     5641 ttaatcgttg ctctcctgta ccatattcgt cggcgtgcta aacaggaaac gggtagcaaa
     5701 ggccaatacc acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa
     5761 tcgctggtaa gctgcaattg ccataccgct aatttgcgac tctgtcgtct gctgtgccac
     5821 tacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatcc cggttaatac
     5881 gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttcggc
     5941 aaagcccatt agcgccagcc cggataccat cacgcccatt gacgcttgac cgtcagctgc
     6001 tgcatgtcgg gcatcaaatg ccagcaacat aaagccacaa gccatcagca gtaagccaaa
     6061 ggcaaacttc agccagacgc gcaatgttga gttgccgtgg ctttctggcg acgccagcca
     6121 ggccagaaca accccagcga gcatcaccgc aattgcattc accgactgga atagtgcagt
     6181 aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt
     6241 actgccgccc tgttgtgcca gtacccagaa caatgtcccg acaaacatca acaatacaat
     6301 ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa tgatttgtgc
     6361 ggcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt
     6421 aaaaaatact ggggctaaac agagcatcac cactaaccag ctccataccg gtaaggcaaa
     6481 tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat gacgatgccc
     6541 gcttaagaaa atcaacaaac cgataaacat gccgccaccc gcaagggcaa agccaacatg
     6601 ccatccatac cactgagcag ccaggccgca ggcgattggg gcggcgatag aaccgatatt
     6661 gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtagag
     6721 ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa taatgattgc
     6781 cagcgccaga tacaggctaa aggttgaatt tgtatcaata cccagcacca catggccaag
     6841 ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc ggtcggcaag
     6901 ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga acaggctgat
     6961 ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta aggcacgcat
     7021 gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata tcgcgcgcgg
     7081 ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg caagccactt
     7141 cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg gtatagcggc
     7201 catcagcctg acggtatgca ccgtgaatat cggtttcaaa gcccggatag tgagcgccga
     7261 tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg atcatttcac
     7321 ccggcattac cagaggaact cccggcggat acggaaggat catattggcg ttaatacgac
     7381 cgaccatttc gtcgaggtaa acttcttcgg tcataccgtg cagctctttc tggaatgcag
     7441 catacggagt cattaccatc gtcggcagca cttcaaatgc gcgatacatc agatccggca
     7501 gattgtggtg aacaatcagt ttgtggatat tctgagccag ttcctgaata cgcatgtttt
     7561 catagaattc aggatcttca cgatacagag acggcagcat gtttttcaca cgcaggttca
     7621 ggtcgaacgc acgtttgaag tcagtcagag cacgcagcag gctcagtgct ttggtcttat
     7681 cgataccgat gctgaacagg aacagcaggt tatacggacc ggttttctca acaacgatgc
     7741 catgttcgtc gaggtatttc gccacgatgc tggccggaat accaaagtcg ctcatggtgc
     7801 cgtctttttc catccccgga gtcagcaggg tgactttgat cgggtcaaga tacatgtgct
     7861 cgttatcgat gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc cagcattcag
     7921 tcgtatcgat atgatccggc tgccatacat caaagaacca gccatcagat tccgttctca
     7981 gacgtttgat ctctttacgg aatttgatcg cacgttcaat ggaaccgttg atcagacgct
     8041 tacctgcatt gcctttcatc atcgccgcag cggtttcagt ggacgccacg ataccgtagt
     8101 gcggagaagt ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg tttacgtcac
     8161 ctttaacgtg gatcatggaa gcctgagaga acgccgccag cagtttgtga gtggactggg
     8221 tttcgtaaat cactttccct tctacacggc caccgctcat accgcattta ccttcgtaaa
     8281 tcggtgagaa gttggtgtaa ggcacccacg cggagtcaaa gtggatggat ttcacatcca
     8341 gtgttttctt gatgaagtcg gtgttgtaca gcagaccatc ataggtagag ttggtaatta
     8401 cagcatgtac cggccaggtt gcgtttggtg tttctttcac gcgcttagca atggtagcgt
     8461 gctggaattc actctgtggg ataccaccaa gaataccgta agcgttacgg gtcgggcgga
     8521 aatagattgg cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg tggcagttac
     8581 ggtcaatcag aatggtgctg cctgccggag cagagtacat accaacaatt ttgttcgcag
     8641 tggaagtacc gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga gcgatatact
     8701 gttctgcttc tttgtgtgga ccactgtgat ccagcagaga acccagttca gatactgaaa
     8761 tggaaatatc agatttcatg gtattcggac caaagaaatc atagaacagg ctacctaccg
     8821 ggcttttctg gaatgcagta ccacccatgt gaccaggagt acagaaagta tatttacctt
     8881 cacgaacata tttaaacagt gctttagtca gcggaggcag aatagtgttg atatattcgt
     8941 cagtggtctg cttgatcttg ttagcaatat cttcagcagc acccagcgca tattcaaaga
     9001 agctaatctg taaacgcagg tcattcaggc ttacatcgag agtggaatac gtattagcga
     9061 acgcgtacaa cggcaggttc tcgttcattt tgctaatttc ttcgcacagc tcgagattat
     9121 atttatccca gtcaaaaata acgccgcaca gacgcgcatt gttttcgatc agttttaata
     9181 agtcgtcacg gtcgttcggg taaacaatct ggaagttcag acgttcaagc gcgcgatgaa
     9241 gttcacggat gggttcttct ttaaaataaa cccccatgtg attcaatatt gcaataacgt
     9301 tcatagtcat atctccaggt aaaaaaggcc cctcccaaca catgggacaa aatgaaagga
     9361 ggagcctcgg aaaatacttt taattaatgt gcgttagacg cggtgtggtt atccattgag
     9421 tggctctggc gctcgtgcat tttgcgagcg tagaacatca ggataatcag gctgacgatg
     9481 aaggtacctg ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca gaacacgcaa
     9541 cccagtacag agcagatcag gctgacaaag ttgcggatgt taacgccttc aaaacgaatc
     9601 aggtcaacgc aagagtagaa gtacggcagc atagtcagca gtactgcgat accggtcagt
     9661 tcaccgaaca ggtcagatgc tttaccaccg gcagagttca tcagagtgat gaggatcatc
     9721 agggcagtca ttttcactgc agccagcagc agaccttttt tcggaatacc gttgctgtcg
     9781 acttcaccat aaactttcgg gaagttaccg tcgttagcgg cacgtacacc tgcctggcct
     9841 accaacatca tccaggagcc cagagaagtc aggcacgcaa aggcggtgaa tgcagaaacc
     9901 agcggcgcag cccagttacc gaggatagtt gaagcactga ttgcaaacgg agcaccggaa
     9961 gccgccatta cagaagacgg atacataccg gaaagcacct gagtcgcagc gatgtaaaca
    10021 atacctgcta aaccagtacc cagcatggtt gccagcggaa cggtacgttt cgggttttta
    10081 accataccag tacttacagc tgcggattca acacccacga aggcccacag gcagagcaga
    10141 atacttttaa tgatcgcatg accatcagtg gtatccgcag tattccagtt agctgcataa
    10201 gttgccgcat caaaccaatg ccagccaaca atagcagtca tcaccacagg aataagaacc
    10261 agcaccagac caatagtggt taaacggctt acccaggtac cgccgagcat atttacaaag
    10321 gtaaataccc agacgatagc aatacaggcg atacccgccg gaacaggatc atttaatact
    10381 gggaagaagg tggaaagata agatacagcg gtaataccaa tcgccaggtt accaatccag
    10441 ttagcatggt aataaagaac acctgtctga aaaccaaatg caggagaaat ttctccggca
    10501 taagcaattg ggccaccttg ttgcgggttt tttgttgcca gtcgggcata tacatacgcc
    10561 agcgacattg caccaataat agagataatc caaccccaga tagcaatacc accgatactt
    10621 gctaggttcg caggtaataa tgcaataccg ctccccatca tattaccggc aacaacaccg
    10681 gtacaggcaa atagcccgat cttcttggca gaactcatgc tcttctccta atttcatttt
    10741 tgaatttgga gtccgggtca tgatgtataa ctatttcctg accagaccaa actggcgcta
    10801 agattactca cgaaaaaagg attaatccta aagattaggt gaaataacac aaaagtttct
    10861 gtaagtgaga acttgaggtt ttttattaac acatcaggat cgcaagttga tatcatgaaa
    10921 agataaacat tcaatgttta caatagattg cgtgacgtct tctggttaaa tttatgtaat
    10981 aaaaattatg cggcaaataa attgccgcaa catattatac caacaggaac atacaaaaac
    11041 tcaacaacaa atattcccga gcatgaatca acccgggggt tacttattct gaagcgagaa
    11101 atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca ttttcaatcc
    11161 agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca tcagccgctt
    11221 cccggttcat ccccttcatt tcataaacct tgccaagcaa tacataattt agccaggaca
    11281 tttcaagatc aatgccagta tttatcgcct ggtaagactc atctgtttta ccttttacca
    11341 gagcactgac cgcttttatt tgatatataa tggacaggtt attcaattcc ggtagtgtaa
    11401 caatgttatc tatttctgtg ttcagtgctg ctaattgttt ttcatctaaa ggatgttgag
    11461 aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat tctggggatg
    11521 attgaacaat ctcacctaat aattcactgg cacggttcaa tgatttatca tcgccatgca
    11581 gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatga ggcaaaattt
    11641 gctggagcgt ctcctgcatt cgttgtggcc acggttggtt taacgctttt gataaactct
    11701 ccagtaaatc attttgaatc gccagctgat taccgttagt gatgacataa cgtttatcca
    11761 gcatggttga accatctgca ttgtctacca attttatcga cataaagcat tgttgagcac
    11821 ggtattggcg ctgattaaca aacgcaatag ataatgtttt accggaactg ctcggttcat
    11881 caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta agtgatgtcg
    11941 ccaccaaatc acccacgcct atcgcgtaag agagctgata cggggaactc cagctgttac
    12001 aacttttatt taccatatta atgtcaatat cgcgtggatt gagcaaaata cgcgatttgc
    12061 tcatcggaag acgtgtatca agacttgaaa acgctaccag tgctacacag atacctaagg
    12121 acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta actggggatt
    12181 gttcaggtgg cgttgcggtg ttttgaatgt taagactgtg ggagggagaa tctgtggcag
    12241 gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc tcttcttcgc
    12301 tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta gcgatataga
    12361 caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata ctctgcgtca
    12421 caacgtgatt ggtgacaata cttctcttcc agacattatc gataagttca tccctgctaa
    12481 gtacttcgcc actgtgttga gcaaagaaaa ccagaaggtc gattaatctc ggctcaaggg
    12541 taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc cattcgccaa
    12601 cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga tgcaatgata
    12661 attg
//
LOCUS       DAZFKY010000139        12419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFKY010000139 DAZFKY010000000
VERSION     DAZFKY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(338..1621)
                     /gene="gltA"
                     /locus_tag="ACHKCL_003473"
     CDS             complement(338..1621)
                     /gene="gltA"
                     /locus_tag="ACHKCL_003473"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5764366.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1827..1934)
                     /locus_tag="ACHKCL_003474"
     CDS             complement(1827..1934)
                     /locus_tag="ACHKCL_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764367.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2315..2719
                     /gene="sdhC"
                     /locus_tag="ACHKCL_003475"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2315..2719
                     /gene="sdhC"
                     /locus_tag="ACHKCL_003475"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5764368.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2713..3060
                     /gene="sdhD"
                     /locus_tag="ACHKCL_003476"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2713..3060
                     /gene="sdhD"
                     /locus_tag="ACHKCL_003476"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5764369.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            3060..4826
                     /gene="sdhA"
                     /locus_tag="ACHKCL_003477"
     CDS             3060..4826
                     /gene="sdhA"
                     /locus_tag="ACHKCL_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5764370.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4842..5558
                     /gene="sdhB"
                     /locus_tag="ACHKCL_003478"
     CDS             4842..5558
                     /gene="sdhB"
                     /locus_tag="ACHKCL_003478"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5764371.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5859..8660
                     /gene="sucA"
                     /locus_tag="ACHKCL_003479"
     CDS             5859..8660
                     /gene="sucA"
                     /locus_tag="ACHKCL_003479"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5764372.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8675..9892
                     /gene="odhB"
                     /locus_tag="ACHKCL_003480"
     CDS             8675..9892
                     /gene="odhB"
                     /locus_tag="ACHKCL_003480"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5764373.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9986..11152
                     /gene="sucC"
                     /locus_tag="ACHKCL_003481"
     CDS             9986..11152
                     /gene="sucC"
                     /locus_tag="ACHKCL_003481"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5764374.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11152..12021
                     /gene="sucD"
                     /locus_tag="ACHKCL_003482"
     CDS             11152..12021
                     /gene="sucD"
                     /locus_tag="ACHKCL_003482"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5764375.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         3007 a         3134 c         3390 g         2888 t
ORIGIN      
        1 aatagtggtt ttttgttcat gtaataatat taatgttgct acattatgtt tgagagtttc
       61 tcaaagctct cgaatcataa ataaagtgaa ttaatcctat tacgagttta tttaattgtg
      121 ggtgctaccc aatgtattct acatcgattg gcgggataac ggtggtggtg aatgaaaaat
      181 taggatttta tttagttatc ggatatgtat tcttgtaacg tgtaattatg tatttgtgtc
      241 cgattacgtt tttttgtcga tagacataac taacgaacaa aaaaaagccc gccagatatt
      301 caggcgggct aaacgatata tggttcaaat cagataatta atgtttaaca tctgatttaa
      361 agtctcgttt ttcatagccg gtgtacaact ggcgcggacg ggcgatcttc atgccgtcac
      421 tgtgcatttc gctccagtga gcgatccagc caacggtacg ggccatagcg aagatgacgg
      481 tgaacatgga agacggaata cccatcgctt tcaggatgat accagagtag aaatcgacgt
      541 tcgggtacag tttcttctcg ataaagtacg ggtcgttcag cgcgatgttt tccagctcca
      601 tagccacttc cagcaggtca tctttggtgc ccagctcttt cagaacttca tggcaggttt
      661 cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac gcggtgaccg aagcccatca
      721 ggcggaaaga atcattttta tctttcgcac gacgaacaaa ttccggaatg tgtttaacgg
      781 agctaatttc ttccagcatt ttcagcgccg cttcgttagc accaccgtgt gcaggccccc
      841 acagtgaagc aatacctgct gcgatacagg caaacgggtt cgcacccgaa gagccagcgg
      901 tacgcacggt ggaggtagag gcgttctgtt catggtcagc gtgcaggatc agaatacggt
      961 ccatagcacg ttccagaatc ggattaactt catacggttc gcacggcgtg gagaacatca
     1021 tattcaggaa gttaccggca taggagagat cgttgcgcgg ataaacaaat ggctgaccaa
     1081 tggaatactt gtaacacatc gctgccatgg tcggcatttt cgacagcagg cggaacgcgg
     1141 caatttcacg atgacgagga ttgttaacat ccagcgagtc gtgatagaac gccgccagcg
     1201 cgccggtaat accacacatg actgccattg gatgtgagtc gcgacggaaa gcgtggaaca
     1261 gacgggtaat ctgctcgtgg atcatggtat gacgggtcac cgtagtttta aattcgtcat
     1321 actgttcctg agtcggtttt tcaccattca gcaggatgta acaaacttcc aggtagttag
     1381 aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag caaaatacct tcatcaccat
     1441 caataaaagt aattttagat tcgcaggatg cggttgaagt gaagccaggg tcaaaggtga
     1501 acacaccttt tgaaccgaga gtacggatat caataacatc ttgacccagc gtgcctttca
     1561 gcacatccag ttcaacagct gtgtccccgt tgagggtgag ttttgctttt gtatcagcca
     1621 tttaaggtct ccttagcgcc ttattgcgta agactgccgg aacttaaatt tgccttcgca
     1681 catcaacctg gctttacccg tttgttattt ggctcgccgc tctgtgaaag aggggaaaac
     1741 ctgggtacag agctctgggc gcttgcaggt aaaggatcca ttgatgacga ataaatggcg
     1801 aatcaagtac ttagcaatcc gaattattaa acttgtctac cactaataac tgtcccgaat
     1861 gaattggtca atacttccac actgttacat aagttaatct taggtgaaat accgacttca
     1921 taacttttac gcattatatg cttttcctgg taatgtttgt aacaactttg ttgaatgatt
     1981 gtcaaattag atgattaaaa attaaataaa tgttgttatc gtgacctgga tcactgttca
     2041 ggataaaacc cgacaaacta tatgtaggtt aattgtaatg attttgtgaa cggcctatac
     2101 tgccgccagg tctccggaac accctgcaat cccgagccac ccagcgttgt aacgtgtcgt
     2161 tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta tagaaaggac gctgtctgac
     2221 ccgcaagcag accggaggaa ggaaatcccg acgtctccag gtaacagaaa gttaacctct
     2281 gtgcccgtag tccccaggga ataataagaa cagcatgtgg gcgttattca tgataagaaa
     2341 tgtgaaaaaa caaagacctg ttaatctgga cctacagacc atccggttcc ccatcacggc
     2401 gatagcgtcc attctccatc gcgtttccgg tgtgatcacc tttgttgcag tgggtatcct
     2461 gctgtggctt ctgggtacca gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat
     2521 tatgggcagc ttcttcgtca aatttatcat gtggggcatc cttaccgctc tggcgtatca
     2581 cgtcgtcgta ggtattcgcc acatgatgat ggattttggc tatctggaag aaacattcga
     2641 agcgggtaaa cgctccgcca aaatctcctt tgttattact gtcgtgcttt cacttctcgc
     2701 aggagtcctc gtatggtaag caacgcctcc gcattaggac gcaatggcgt acatgatttc
     2761 atcctcgttc gcgctaccgc tatcgtcctg acgctctaca tcatttatat ggtcggtttt
     2821 ttcgccacca gtggcgagct gacatatgaa gtctggatcg gtttctgcgc ctctgcgttc
     2881 accaaagtgt tcaccctgct ggcgctgttt tctatcttga tccatgcctg gatcggcatg
     2941 tggcaggtgt tgaccgacta cgttaaaccg ctggctttgc gcctgatgct gcaactggtg
     3001 attgtcgttg cactggtggt ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga
     3061 tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg tgccggcggc gcaggtatgc
     3121 gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc gctgctctct aaagtcttcc
     3181 cgacccgttc ccataccgtt tctgcgcaag gcggcattac cgttgcgctg ggtaataccc
     3241 atgaagataa ctgggaatgg catatgtacg acaccgtgaa agggtcggac tatatcggtg
     3301 accaggacgc gattgaatat atgtgtaaaa ccgggccgga agcgattctg gaactcgaac
     3361 acatgggcct gccgttctcg cgtctcgatg atggtcgtat ctatcaacgt ccgtttggcg
     3421 gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac tgcggcagcg gctgaccgta
     3481 ccggtcacgc actgttgcac acgctttatc agcagaacct gaaaaaccac accaccattt
     3541 tctccgagtg gtatgcgctg gatctggtga aaaaccagga tggcgcggtg gtgggttgta
     3601 ccgcactgtg catcgaaacc ggtgaagtgg tttattttaa agcccgcgct accgtgctgg
     3661 cgactggcgg agcagggcgt atttatcagt ctaccactaa cgcccacatt aacaccggcg
     3721 acggcgtcgg catggctatc cgcgcaggcg taccggtgca ggatatggaa atgtggcaat
     3781 tccacccgac cggcattgcc ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag
     3841 gcggttatct gctgaacaaa catggcgaac gctttatgga gcgttatgcg ccgaacgcca
     3901 aagacctggc gggccgtgat gtggtggcgc gttcaatcat gattgaaatc cgtgaaggtc
     3961 gcggctgtga tggtccgtgg gggccacacg cgaaactgaa actcgatcac ctgggtaaag
     4021 aagttctcga atcccgtctg ccgggtatcc tggagctttc ccgtaccttc gctcacgttg
     4081 atccggtgaa agagccgatt ccggttatcc caacctgtca ctacatgatg ggcggtattc
     4141 cgaccaaagt taccggtcag gcgctgactg tgaatgagaa aggcgaagat gtggttgttc
     4201 cggggctgtt cgcggtaggt gaaattgcct gcgtatcggt acacggtgct aaccgtctgg
     4261 gcggcaactc gctgctggac ctggtggtct ttggtcgcgc agcgggtctg catctgcaag
     4321 agtctatcgc cgagcagggc gcattgcgcg atgccagcga gtctgatgtt gaagcatctt
     4381 tggatcgtct gaaccgctgg aacaataatc gtaacggtga agatccggtg gcgattcgta
     4441 aagcgctgca agaatgtatg cagcataact tctcggtctt ccgtgaaggt gatgcgatgg
     4501 ctaaagggct tgagcagttg aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg
     4561 acacttccag cgagttcaac acccagcgcg ttgagtgcct ggaactggat aacctgatgg
     4621 aaacggcgta tgcaacggct gtttctgcca acttccgtac cgaaagccgt ggcgcacata
     4681 gccgcttcga cttcccggat cgtgatgatg aaaactggct gtgccactcc ctgtatctgc
     4741 cagagtcgga atccatgacg cgccgaagcg tcaacatgga accgaaactg cgcccggcat
     4801 tcccgccgaa gattcgtact tactaatgcg gagacaggaa aatgagactc gagttttcaa
     4861 tttatcgcta taacccggat gttgatgatg ctccgcgtat gcaggattac accctggaag
     4921 cggaagaagg tcgcgacatg atgctgctgg atgcgcttat ccagctgaaa gagaaagatc
     4981 ccagcctgtc gttccgccgc tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca
     5041 tgaacggcaa gaatggtctg gcctgtatta ccccgatttc ggcactcaac cagccgggca
     5101 agaagattgt gattcgcccg ctgccaggtt taccggtgat ccgcgatttg gtggtagaca
     5161 tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta cctgttgaat aatggacaaa
     5221 atccgccagc tcgcgagcat ttacagatgc cagagcagcg cgaaaaactc gacgggctgt
     5281 atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg
     5341 ataagtttat cggcccggca ggcttgttag cggcatatcg tttcctgatc gatagccgtg
     5401 ataccgagac tgacagccgc ctcgacggtt tgagcgatgc attcagtgta ttccgctgtc
     5461 atagcatcat gaactgcgtc agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg
     5521 gccatatcaa gtcgatgttg ttgcaacgta atgcgtaaac cgtaggcctg ataagacgcg
     5581 caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa
     5641 gtcattaccc gtaggcctga taagcgcagc gcatcaggcg taacaaagaa atgcaggaaa
     5701 tctttaaaaa ctgcccctga cactaagaca gtttttaaag gttccttcgc gagccactac
     5761 gtagacaaga gctcgcaagt gaaccccggc acgcacatca ctgtgcgtgg tagtatccac
     5821 ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag
     5881 cctggttgga ctcttcttac ctctctggcg caaaccagag ctggatagaa cagctctatg
     5941 aagacttctt aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt
     6001 tacctggtac gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc
     6061 gcctggcgaa agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga
     6121 agcaggtaaa agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga
     6181 atctcgatcc gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc
     6241 acgatctgac cgaagcagac ttccaggaga ccttcaacgt cggttcattt gccagcggca
     6301 aagaaaccat gaaactcggc gagctgctgg aagccctcaa gcaaacctac tgcggcccga
     6361 ttggtgccga gtatatgcac attaccagca ccgaagaaaa acgctggatc caacagcgta
     6421 tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa acgcttccta agcgaactga
     6481 ccgccgctga aggccttgaa cgttacctcg gtgcaaaatt ccctggcgca aaacgcttct
     6541 cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga gatgattcgc cacgctggca
     6601 acagcggcac ccgcgaagtg gttctcggga tggcgcaccg tggtcgtctg aacgtgctgg
     6661 tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga gtttgccggt aaacataaag
     6721 aacacctcgg cacgggtgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg
     6781 atggcggcct ggtacatctg gcgctggcgt ttaacccgtc tcaccttgag attgtcagcc
     6841 cggtcgttat cggttctgtt cgtgcccgtc tggacagact tgatgagccg agcagcaaca
     6901 aagtgctgcc aatcaccatt cacggtgacg ccgcagtgac cgggcagggc gtggttcagg
     6961 aaactctgaa catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta
     7021 tcaacaacca ggttggcttc accacctcta acccgctgga tgcccgttca acgccgtact
     7081 gtactgatat cggtaagatg gttcaggcac cgatttttca cgttaacgcg gatgatccgg
     7141 aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg
     7201 tcttcatcga cctggtgtgc taccgccgtc acggccacaa cgaagccgac gagccgagcg
     7261 caacccagcc gctgatgtat cagaaaatca aaaaacatcc gacgccgcgc aaaatctatg
     7321 ctgacaagct ggagcaggaa aaagtggcga cgctggaaga tgccaccgag atggttaacc
     7381 tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc agagtggcgt ccgatgaaca
     7441 tgcactcttt cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaata
     7501 aagttgagat gaagcgcctg caggagctgg cgaaacgcat cagcacggtg ccggaagcgg
     7561 ttgaaatgca gtctcgcgtt gccaaaattt atggcgatcg ccaggcgatg gctgccggtg
     7621 agaaactgtt cgactggggc ggcgcggaaa acctcgctta cgccacgctg gttgacgaag
     7681 gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg
     7741 cggtgatcca caaccagtct aacggttcca cttacacgcc gctgcaacac atccataacg
     7801 gccagggcgc gttccgtgtc tgggactccg tactttctga agaagcagtg ctggcgtttg
     7861 aatacggtta tgccaccgca gaaccacgca ctctgactat ctgggaagct cagttcggtg
     7921 acttcgccaa cggtgcgcag gtggttatcg accagttcat ctcctctggc gaacagaaat
     7981 ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg
     8041 agcactcctc cgcgcgtctg gaacgttatc tgcaactttg tgctgagcaa aacatgcagg
     8101 tgtgcgtacc gtctaccccg gcacaggttt accacatgct gcgtcgtcag gcgctgcgcg
     8161 ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg
     8221 tttccagcct cgaagaactg gcgaacggca ccttcctgcc agccatcggt gaaatcgacg
     8281 agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgacc
     8341 tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac
     8401 tctacccgtt cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg
     8461 attttgtctg gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc
     8521 atttccgtga agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg
     8581 cctctccggc ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg
     8641 acgcgctgaa cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc
     8701 tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggtga
     8761 cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt
     8821 accggcatca gcagacggca ttctggatgc ggttctggaa gatgaaggta caacggtaac
     8881 gtctcgtcag atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc
     8941 caaatctgaa gagaaagcgt ccactccggc gcaacgccag caggcgtctc tggaagagca
     9001 aaacaacgat gcgttaagcc cggcgatccg tcgcctgctg gctgaacata acctcgacgc
     9061 cagcgccatt aaaggcaccg gtgtgggtgg tcgtctgacc cgtgaagatg tggaaaaaca
     9121 tctggcgaaa gctccggcga aagagtctgc accggcagcg gctgctccgg cggcgcaacc
     9181 ggctctggct gcacgtagcg aaaaacgtgt cccgatgact cgcctgcgta agcgtgtggc
     9241 agagcgtctg ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa
     9301 catgaagccg attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg
     9361 catccgtctg ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta
     9421 cccggaagtg aatgcttcta tcgacggcga tgacgtggtt taccacaact atttcgacgt
     9481 cagcatggcg gtttctacgc cgcgcggcct ggtgacgccg gtactgcgtg atgtcgatac
     9541 cctcggcatg gcagacatcg agaagaaaat caaagagctg gcagttaaag gccgtgacgg
     9601 caagctgacg gttgaagatc tgaccggtgg taacttcacc atcaccaacg gtggtgtgtt
     9661 cggttccctg atgtctacgc cgatcatcaa cccgccgcag agcgcaattc tgggtatgca
     9721 cgctatcaaa gatcgtccga tggctgtgaa tggtcaggtt gagatcctgc cgatgatgta
     9781 cctggcgctg tcctacgatc accgtctgat cgatggtcgc gaatccgtgg gcttcctggt
     9841 aacgatcaaa gagttgctgg aagatccgac gcgtctgctg ctggacgtgt agtagtttaa
     9901 gtttcacctg cactgtagac cgacgtggat agggtgtcgg tctacggttt aaaagataac
     9961 gattactgaa ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactgtt
    10021 tgcccgctat ggcttaccag caccggtggg ttatgcctgt actactccgc gcgaagcaga
    10081 agaagccgct tcaaaaatcg gtgccggtcc gtgggtagtg aaatgtcagg ttcacgctgg
    10141 tggccgcggt aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt
    10201 tgcagaaaac tggctgggca agcgtctggt aacgtatcaa acagatgcca atggccaacc
    10261 ggttaaccag attctggttg aagcagcgac cgatatcgct aaagagctgt atctcggtgc
    10321 cgttgtagac cgtagttcac gtcgcgtggt ctttatggcc tccaccgaag gcggcgtgga
    10381 aatcgaaaaa gtggcggaag aaactccgca cctgatccac aaagttgcgc ttgatccgct
    10441 gactggcccg atgccgtatc agggacgcga gctggcgttc aaactgggtc tggaaggtaa
    10501 actggttcag cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga
    10561 cctggcgctg atcgaaatca acccgctggt catcaccaaa cagggcgatc tgatttgcct
    10621 cgacggcaaa ctgggcgctg atggcaacgc cctgttccgc cagcctgatc tgcgcgaaat
    10681 gcgtgaccag tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta
    10741 cgttgcgctg gacggtaaca tcggttgtat ggttaacggc gcaggcctgg cgatgggtac
    10801 gatggacatc gttaaactgc acggcggcga accggctaac ttcctggacg ttggcggcgg
    10861 cgcaaccaaa gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa
    10921 agccgttctg gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat
    10981 catcggtgcg gtagcggaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa
    11041 caacgccgaa ctcggcgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa
    11101 aggtctgacg gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt
    11161 ttaattgata aaaacaccaa ggttatctgc cagggcttta ccggtagcca ggggactttc
    11221 cactcagaac aggccattgc atacggcact aaaatggttg gcggcgtaac cccaggtaaa
    11281 ggcggtacta cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct
    11341 actggcgcta ccgcttctgt tatctacgta ccagcaccgt tctgcaaaga ctccattctg
    11401 gaagccatcg acgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg
    11461 gatatgctga ccgtgaaagt gaagctggat gaagcaggcg ttcgtatgat cggcccgaac
    11521 tgcccaggcg ttatcactcc gggtgaatgc aaaatcggta ttcagcctgg tcacattcac
    11581 aaaccgggta aagtgggtat cgtttcccgt tccggtacac tgacctatga agcggttaaa
    11641 cagaccacgg attacggttt cggtcagtcg acctgtgtcg gtatcggcgg tgacccgatc
    11701 cctggctcta actttatcga cattctcgaa atgttcgaaa aagatccgca gaccgaagcg
    11761 atcgtgatga tcggtgagat cggcggtagc gctgaagaag aagcagctgc gtacatcaaa
    11821 gagcacgtta ccaagccagt tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa
    11881 cgtatgggcc acgcgggtgc catcattgcc ggtgggaaag ggactgcgga tgagaaattc
    11941 gctgctctgg aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca
    12001 ctgaaaactg ttctgaaata aatatctgta ataagaaata gccctcgccg cttccctcta
    12061 caggaatggc gaagggctgt cggtttcgac atggttggcc atcgtatgat ggcctttttt
    12121 atgccttaaa cggttaacca cggtcgaaat ctgactgcag gcttgactgc atgaccacag
    12181 ccactgtgga ttgccgcaac cgaccgcaag ccacgaataa gaacgacggc gaatttcgaa
    12241 tcgttacctg atgcactggc ttcccagcgt ttgaccgtag cgttaagtaa atcaacaaaa
    12301 gcgatgttca ccgcttttcg tcaagtcgtg ccatttgtca gccaggatcg ggtctttacc
    12361 ccggatattg cagcatcgca aaaattctta gagggatatg ctataaatca aaaataaaa
//
LOCUS       DAZFKY010000140        12340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFKY010000140 DAZFKY010000000
VERSION     DAZFKY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKCL_003483"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKCL_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5764376.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHKCL_003484"
     CDS             516..1217
                     /locus_tag="ACHKCL_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5764377.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKCL_003485"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKCL_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5764378.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHKCL_003486"
     CDS             complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHKCL_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5764379.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2876..4207)
                     /locus_tag="ACHKCL_003487"
     CDS             complement(2876..4207)
                     /locus_tag="ACHKCL_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5764380.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHKCL_003488"
     CDS             complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHKCL_003488"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5764381.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCEPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5918..6307)
                     /locus_tag="ACHKCL_003489"
     CDS             complement(5918..6307)
                     /locus_tag="ACHKCL_003489"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5764382.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHKCL_003490"
     CDS             complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHKCL_003490"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5764383.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHKCL_003491"
     CDS             complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHKCL_003491"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5764384.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHKCL_003492"
     CDS             complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHKCL_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5764385.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHKCL_003493"
     CDS             complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHKCL_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5764386.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3165 a         3355 c         2955 g         2865 t
ORIGIN      
        1 cgcccccgcc agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca acaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcattt aatcgcctcg ccagagggtg
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcacgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcacga tacccaaaac ctggcgttta tctggccttg ctcgccataa
     1561 tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg
     1621 caatcgactg ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat
     1681 ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc
     1741 agctgttcga cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg
     1801 ccatcgagat ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca
     1861 ttgctgcggt cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa
     1921 ttgcgctcaa atgagaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt
     1981 gttctgctac caccaccacg ggcattgttg cggtgatccc ctggttaccc gagccggagt
     2041 tactcattgc cggaagcgtg gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa
     2101 tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg
     2161 tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta
     2221 gcttcgcgga atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca
     2281 ggatctcagc cagcgtcgtt ctggaaagca ccgatagcgg agactcttgc tcgccctctg
     2341 ccacacacgc ctgctgggta aacaccacac catcgtgcgt ctcaatatgc acaatgttgg
     2401 tatgcccgcc gacgatagtg acacacgccc acttctcacc gttccagact ttggcgcgtg
     2461 agaagaggat ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca
     2521 gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt
     2581 tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc
     2641 cgggaacggt gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac
     2701 gttcaaccgg accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg
     2761 gttcagtaca tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc
     2821 ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag
     2881 aaaatgccag gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag
     2941 acatcccttt gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc
     3001 accctaccaa gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca
     3061 gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat
     3121 tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg
     3181 ttgcttcgcg aaaacctaaa tagacgccaa agaatgcagt catgacagca aagatattga
     3241 gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa
     3301 tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca
     3361 gcgtgaacga cacggcgtag aaaaagacga tgataaacaa aatgccaaac gcgatattca
     3421 ttgcccgcaa tgctttatgc cgcgctactt caatcgattt ttcccgcgag cgataagaga
     3481 tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa
     3541 taatagcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc
     3601 acattcccac catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc
     3661 cagtcgaaat tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa
     3721 tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat
     3781 gcaggtagga agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca
     3841 gcattacgaa atagagtgca cctaacagga tgccccagtt tttacctaaa taaccgctaa
     3901 tgacgctcgg gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct
     3961 gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca
     4021 tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca
     4081 taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac
     4141 tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa
     4201 tttccataat ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca
     4261 aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc
     4321 gaggtcagag aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt
     4381 ctactaaatt aatcgcaaga gaatattcac tctctatttg cggcagatca caaaaaaaag
     4441 gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta
     4501 atggccagtc cgccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca
     4561 tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc
     4621 atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt
     4681 tgtacctgtc cggcaaccgg ctcgcaggtc agtccaaggt tatgctccat cgcgatttcc
     4741 gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc
     4801 attgaacagg ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg
     4861 ttcattttat acagcgcgcc aatagccccg gctgcaagaa aatagcgggc aattgaccgc
     4921 tcgtttaccg gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac
     4981 gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc
     5041 agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg
     5101 gaaaccagct gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc
     5161 acgccttcag tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca
     5221 aaaccggcgt caatttccgc tttactgcgc agcgctagct cgttgtgcat catcaggcca
     5281 gaaatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg
     5341 taaggtacgg gtgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac
     5401 ccgccgccga cagagtaata ggttttactt aatagctctt cctgtgcctt ccaggcagtg
     5461 atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct
     5521 acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact
     5581 aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt
     5641 cccatgatga tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac
     5701 agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata
     5761 aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt
     5821 ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg
     5881 agcggaacga ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag
     5941 cttcaatttc cagcttcaca tctttcggca aacgcgcgac ctgtacacag ctccgggtcg
     6001 ggtaggtcgc ctgatgctca tcgaagaatt gcttatagac ttcgttgatg gtggcaaaat
     6061 cattcagatc ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag
     6121 caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg
     6181 ggatctcacc ggtctgtggg caaaccggaa tttgaccgga ggtgaagacc atgctgccta
     6241 aatcaacgcc ttgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct
     6301 ttttcataca tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgc
     6361 tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg
     6421 ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg
     6481 acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg
     6541 ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg gacgattgag
     6601 aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat
     6661 gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt
     6721 gttccggcgc gacgaccgtc cggcgtgtta ccggttttct ggccgtacac cacgttagaa
     6781 gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt
     6841 ttcttcataa aacgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg
     6901 ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt tttgtcacgg
     6961 attggtttca cgcgggcata tttgatcgca gacagggagt ccgttgccac cgacaggccc
     7021 gcgatgccgc acgccatggt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct
     7081 tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc
     7141 gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacatcg
     7201 tccatcaacg gtgccgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg
     7261 ttaattgcgt acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc
     7321 ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca
     7381 gtacgcatca gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg
     7441 gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
     7501 ggtgcaggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
     7561 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
     7621 aacagcgaat caaattccgg cgtacgcaag aagcgcacca tacggatctt catgatgaag
     7681 tgatcgatca gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca
     7741 atgtagatat ccaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc gttctgtgat
     7801 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc
     7861 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
     7921 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
     7981 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
     8041 tacagcgcta cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa
     8101 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
     8161 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
     8221 cgaccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtaacgca
     8281 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
     8341 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
     8401 gttgcgtttt caatacgaat gccttccatc accttttccc acaattccgt ggtggctggc
     8461 gtcgcttcag cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
     8521 tcgcggacat taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg
     8581 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
     8641 caactaaatt atgcaaattc tgcgggtgcg ttaactttgc ctaaatgaat ggcatccaaa
     8701 gcaatcattt tttcttcgtt ggtcggaatt acggcgcaaa tgacacgcgc attttcactg
     8761 gaaacaattc gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg
     8821 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
     8881 ccgccggtga atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga
     8941 cgggcaattc ggtgaacaaa ggttttaatt gccagctgcg cgcgttcgtg accttcatgc
     9001 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
     9061 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
     9121 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
     9181 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
     9241 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag caaagaatgt
     9301 gcgcgcaggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
     9361 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
     9421 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
     9481 ataccactta aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
     9541 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
     9601 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
     9661 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
     9721 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag
     9781 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
     9841 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttatatac
     9901 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
     9961 gtatttcgcc agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga
    10021 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
    10081 aacaacccag gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga
    10141 tactttagtt ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag
    10201 cgttcccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    10261 cgcatattcc agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt
    10321 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    10381 cagcgtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    10441 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    10501 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    10561 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    10621 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga
    10681 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    10741 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    10801 gcgattcagc ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    10861 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    10921 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    10981 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
    11041 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    11101 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    11161 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    11221 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    11281 gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt
    11341 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    11401 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    11461 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    11521 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    11581 gccgtgaacg ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    11641 tgccaccgca ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac
    11701 atcatagaga tcttccataa tttccaggcc aatcgttccc tgaccagcaa tcactttcgg
    11761 atcatcgtaa ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc
    11821 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    11881 cgctactttg gatttcggcg cgccttttgg catcaccact ttaccgtcga tacccagcat
    11941 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    12001 gcgtttttcc gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    12061 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    12121 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    12181 aataatgtca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    12241 cctgaattaa atatgtaggt aaccgtaacc gacacctgca agacagacag gtggattatt
    12301 tatgtttaaa taaaaacaaa taactaacca acttctatta
//
LOCUS       DAZFKY010000141        12339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFKY010000141 DAZFKY010000000
VERSION     DAZFKY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHKCL_003494"
     CDS             complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHKCL_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5764387.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            546..2807
                     /gene="katE"
                     /locus_tag="ACHKCL_003495"
     CDS             546..2807
                     /gene="katE"
                     /locus_tag="ACHKCL_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5764388.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2854..3612)
                     /gene="chbG"
                     /locus_tag="ACHKCL_003496"
     CDS             complement(2854..3612)
                     /gene="chbG"
                     /locus_tag="ACHKCL_003496"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5764389.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3625..4977)
                     /gene="celF"
                     /locus_tag="ACHKCL_003497"
                     /gene_synonym="chbF"
     CDS             complement(3625..4977)
                     /gene="celF"
                     /locus_tag="ACHKCL_003497"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5764390.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5083..5925)
                     /gene="chbR"
                     /locus_tag="ACHKCL_003498"
     CDS             complement(5083..5925)
                     /gene="chbR"
                     /locus_tag="ACHKCL_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5764391.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5933..6283)
                     /gene="chbA"
                     /locus_tag="ACHKCL_003499"
     CDS             complement(5933..6283)
                     /gene="chbA"
                     /locus_tag="ACHKCL_003499"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5764392.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6334..7692)
                     /gene="chbC"
                     /locus_tag="ACHKCL_003500"
     CDS             complement(6334..7692)
                     /gene="chbC"
                     /locus_tag="ACHKCL_003500"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5764393.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7777..8097)
                     /gene="chbB"
                     /locus_tag="ACHKCL_003501"
     CDS             complement(7777..8097)
                     /gene="chbB"
                     /locus_tag="ACHKCL_003501"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5764394.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8397..8735)
                     /gene="osmE"
                     /locus_tag="ACHKCL_003502"
     CDS             complement(8397..8735)
                     /gene="osmE"
                     /locus_tag="ACHKCL_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5764395.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8937..9764
                     /gene="nadE"
                     /locus_tag="ACHKCL_003503"
     CDS             8937..9764
                     /gene="nadE"
                     /locus_tag="ACHKCL_003503"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5764396.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9994..10881
                     /gene="cho"
                     /locus_tag="ACHKCL_003504"
     CDS             9994..10881
                     /gene="cho"
                     /locus_tag="ACHKCL_003504"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5764397.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10841..11416)
                     /gene="ves"
                     /locus_tag="ACHKCL_003505"
     CDS             complement(10841..11416)
                     /gene="ves"
                     /locus_tag="ACHKCL_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5764398.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11619..12104)
                     /gene="spy"
                     /locus_tag="ACHKCL_003506"
     CDS             complement(11619..12104)
                     /gene="spy"
                     /locus_tag="ACHKCL_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5764399.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         3247 a         3128 c         2827 g         3137 t
ORIGIN      
        1 aaggcagctt tttgttacag gtggtttgaa tgaatgtagc aatgaaatag agaatttcag
       61 gccatgtaac tcccggcaaa accgggaggt acgtaatctt tactcagtca cttccccttc
      121 ctggcggatc tgatttgccc aacgctgggc ggattcaggc acagtaaacg ccggtgagcg
      181 cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca tccagacatc
      241 acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc gtgggacata
      301 gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct tcactaaacg
      361 catattaaaa atcagaaaaa ctgtagttta gccgatttag cccctgtacg tcccgctttg
      421 cgtgtatttc ataacaccgt ttccagaata gtctccgaag cggggtctgg ctggtggtct
      481 atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt aaggagacga
      541 gttcaatgtc gcaacaaaac gaaaagaacc cacatcagca ccagtcacca ctacacgatt
      601 ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggctct catcgtccag
      661 cggctgaacc aacaccgcca ggtgcacaac ctaccgcctc agggagcctg aaagcccctg
      721 atacgcgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt gaaaattatg
      781 cgctgaccac taatcagggc gtgcgcatcg ccgacgatca aaactcactg cgtgccggta
      841 gccgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc cactttgacc
      901 atgagcgcat cccggaacgt attgttcatg cacgtggatc agccgctcac ggttatttcc
      961 agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat ccgaacaaaa
     1021 tcaccccagt atttgttagg ttctctaccg ttcagggtgg tgctggctct gccgataccg
     1081 tgcgtgatat ccgtggcttt gccaccaagt tctatactga agagggtatt tttgacctcg
     1141 ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc gattttgttc
     1201 atgcggtaaa accagaacca cactgggcaa ttccacaagg gcaaagcgcc cacgatactt
     1261 tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg gcgatgtcgg
     1321 atcgcggcat cccccgcagt taccgcacca tggaaggctt tggtattcat accttccgcc
     1381 tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca ctggcaggta
     1441 aagcctcact cgtttgggat gaagcacaaa aactaaccgg acgtgacccg gacttccacc
     1501 gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa ctgggcttcc
     1561 agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat ccaactaaac
     1621 ttatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc aatcgcaatc
     1681 cggataactt ctttgccgaa aacgaacaag cagcattcca tccaggtcat attgttcctg
     1741 gtctggactt caccaacgat cccctgttgc agggacgttt gttctcctat accgatacac
     1801 aaatcagtcg tcttggtggg ccgaatttcc atgagattcc gattaaccgc ccaacctgcc
     1861 cttaccataa tttccagcgt gacggcatgc atcgtatggg gatcgacact aacccggcga
     1921 attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg ggaccgaaac
     1981 gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgc gagcgcagtc
     2041 catcttttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag acgccattcg
     2101 agcagcgcca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt cgtccgtata
     2161 ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc caggcggtgg
     2221 cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca cctccggacg
     2281 tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac ggtgatgtga
     2341 aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag atcggcagac cttctggcca
     2401 ttctcaaggc gctgaaggcc aaaggcgttc atgccaaact gctctactcc cgaatgggtg
     2461 aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc ggtgcgcctt
     2521 cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc gctgacaacg
     2581 gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt gcgctggcgg
     2641 gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa gaagggattg
     2701 tggaagctga cagcgccgac ggtagtttta tggatgaact gttaacgctg atggcagcac
     2761 accgcgtgtg gtcacgcatt cctaagattg acaaaattcc ggcgtaaata tggataaggt
     2821 cacgtcccct ggggagtgac cttaccacct tacttacaca tcaagataac tacccaaccg
     2881 ataaccacgc tcagcaattg catatttcaa cgacgctgat gtcagcactt ccagctccgt
     2941 taaccgtggg aaacagtaag cactctggcg aatcgtatta tcgacaaacg ccggatggca
     3001 catgacttcc agcgagcgtt ccccgcgatg gctggaatca tcaagcactt gcaggaacag
     3061 tgcctcgcta atctcttcac cataaaatgc gctactgaat ccgtgcgaac tgcgcagatt
     3121 agccggtaaa tcaccagcgt tagatagcgg ctgacggtcg atgcgcaacg caatcccctc
     3181 ttcagccgca aacctggcaa caatagggaa aatctgcggg aacatatgca catgatgatg
     3241 gctatcaaga tgcgtaggtt tgcgtccaaa tagctcaatg aaacgcaaat actgactggc
     3301 aagctcctga gtaatttctt ccagcggtaa agcatcttct tctgccaact gccagatcca
     3361 ttttcccagc acaccatcgc gggttaaccc cggcatagct gtcagtggct tgcccatagt
     3421 aaggacaaag ttcatcccta tggccagact cggttcatca cgactcaact gcaccgcatg
     3481 gtcaatagcc tgcccattca ccagcgccgt cgtcgatgtg acaatcccat tgcgacaggc
     3541 ctcgataatg ccgtagttct gacctttgct taagccaaaa tcatcggcat taacaatcag
     3601 taagcgttcc ataatcagcc tcggttaatg tgctttttta agctctgcga tgcagtcggc
     3661 aaaatttggc agccatttct cgtgcgccag aatcatctcg cgtgccagca gctcagcatc
     3721 gcgatcggaa tgcaccaacg gactaaggtt tagcgccagt aacacatcgt taaattctcc
     3781 gctaagtgcg gcgttgctgg cagcaatctc gaagccttta atggtgtgaa tcagccccat
     3841 cactttatca tcgaaatgcg taatgcgcgg atgtggcgtc gcgccatcgc gccccagcgt
     3901 acaggtcatt tctaccgccc agtctgccgg aatattatca atatgcccat gatgcgggat
     3961 attaacgtaa tgttctgctt gcttatcgtt gtagatagcg ttgatcactt cgcacgctgc
     4021 atcagagtaa taagccccac cgcgctgttc cagttctttc ggcttaactt tcaactccgg
     4081 gtttttatac agctcaaaaa gttgtttctc gactttctgt actacctgcg ctcgtgcgcc
     4141 gcctttgtag tattcgccca tttcaatagc cagcatctct ttttgcttga agtaataaag
     4201 caaataagaa cacggcaaca gattcagaga acgaattaag ccttcgctaa atggcagatc
     4261 gaaaatattt ttaacgccag atgcttttaa ctgcccggat gccacaccat caagcaattc
     4321 ggcaaagcgc gacttgccat ttaccagcac atccttaatg aacaccatat ggttgaggcc
     4381 gaacagatcg atagataaat catcgctgtc tttcagcatc agaacatcgc gaataaacat
     4441 cttcatgccg atcggaatat tacacacgcc gataaagcgt ttaaatccgg tatgacgata
     4501 aacggcttca gtgaccattc ccgccgggtt agtgaagtta atcacccatg cattcggaca
     4561 aagttcttcg acatctttta cgatgtcaaa aatcaccgga atggtacgca gacctttaaa
     4621 cagaccgccc gcgccgttgg tttcctgacc aagataacca tgacttaatg gaatacgttc
     4681 atccagttca cgcgccggta attggccaac gcgcagttgg gtagtaacga aatcagcatc
     4741 tttcaatgct tcgcggcgat ccagcgtttt ataaagcttc atcgggacgc cagcgttatc
     4801 aatcatccgt tggcagagat cgaaaataat atccagttta gctttgccac cttcgacatc
     4861 caccagccat aattcgctga ccggcaattc gtgataacgc ttaataaatc cttccagtaa
     4921 ctccggggta tagctgctcc cgccaccaat agtgacgact tttaatttct ggctcataat
     4981 ttctcccttc agtacagaat actgatatct ggcatatctg ccccccccgg acataaataa
     5041 tccagcaaca ggacagatat gtgaattgtc aggtataacg acttactgat taaattcagt
     5101 caatttctta cgatagctct taggcgtaaa ggacgttaat ttcttaaacg ttttaataaa
     5161 caaactcggg ctactataac cggcctcaaa cgcaatatcc gtcactgaat agttggtcat
     5221 ttccagttgt tttttggcaa aattaatacg gatttcatta ataatctgca ttggcgtttt
     5281 gccataatat cgttgagtcg cccgcgtcaa atattcctgt gatttggctg acaacgtcac
     5341 catattctcc agcgccgatt cactaaactg ctctttatca tgcatctttt ctaccgtact
     5401 tttcagccac tgcggtacat catcaatcac ctgttcttcg cggtaatgac gtaaacggtt
     5461 aatgacataa aaagtaacca tctcaacaaa ctcttccagc cctgtttcgc ggaaattcaa
     5521 tgaagaaatc actgtttcca cataggtgag aaaagcgtta ttggtccggt atacctgcga
     5581 agctacaaag caataaggca acaatggcag gtaatgctgc tcaaaaaagc gtttactgat
     5641 cccaacgttc aatatgcgcg tggcaccaaa ctcataaaaa ctttgatggt gcgaacctaa
     5701 cggaataaaa acaaaatcgc cccgttccag taacacgcgc ttaccgttaa tctcctggaa
     5761 ataacgcccg gttaatacca gagtaaattc ataatagtcg tgctgatgca gtccgctgat
     5821 actctcagtt ttgttataga taaacacatg gaagtttttg ccattaaaca actgctgttc
     5881 tcgggcagtg gcaatttccg gcgcgttaat cactggctgc atcatcgact ccttatgcct
     5941 tcagtttttc atgaagctca attaattcag taatcagttc acgcgcaagc atggacgtca
     6001 ttaaatgatc ctgagcgtgg accagcacca gactcacttt catcttacct tcgcccgcat
     6061 cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt caatgccatc cgtgactgat
     6121 ccatcatggc ttttgctgcg gcaaaatcgc cctgcttcgc ctgtttcagt gcggcatacg
     6181 ccaagctgcg cgcttgtccg gagttgatga taagccccat caccacttct tccagctctt
     6241 cagcttccgt ttgcgtatcg ggaatattat cgagatccat catacatcgt tcctctcttt
     6301 tcttaccggc acgattaccc gtaccggcat cgattaaaat ttcagggcgt tagcgatatc
     6361 ttcttcgctc tcttctttat caatcgcatt ttgtgctttg ttagccacca caacaaaggg
     6421 cagataaatt aacgttgcga taccaaggtt gaagagtgca accagcaatg cgacgacgct
     6481 accgttggtg ttaaagaagg ctcccagacc ggttggcatg gtccacggtg caatattggt
     6541 caccggagga ataatgccca tgtagtacgc tgccagggtg attgccgcca gaatcggttg
     6601 taccagtaca aacgggataa acatcaccgg gttcatgata attggcagac caaacagaat
     6661 cggttcgtta atctggaaga tgccggacgg cagcgccagt tttgccacct gacgataatc
     6721 agcacggcga gaggcaataa agatagccag gatcaggcct aaagtcgcac cactgcctcc
     6781 aaggaaaata aaggagtcca gcatcggctt ggcccagata tggaaggtct taccggctgc
     6841 cagcgccgct tcgacggaac catattgctg ataggtcgcg atattttcca gcgcccacgg
     6901 cgtcataatg ccgttgtcca gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca
     6961 gagcagtgga acaaagatca cataggccca gcccaccacg ctacccaacg atgccagtgg
     7021 ggttgagatg gtatccataa tgatctgatg gaagttggtg ccccaggtat tcaacgccca
     7081 ggcaataatc cccatcacgg aaagaataat aaagccggga attaatgccg agaaggaacg
     7141 cgatactgaa gcaggtacgc tgtcgggcag tttaatgacc caattgcggc ggacaataaa
     7201 ggtaaacatt tctgccacca ccaggccaat aataatcccg gagatgatat tcgccccacc
     7261 taaccagttt gcgccaaccg cataggcctc accgacacta tatggggtga cggtcataaa
     7321 tgctgcaacg gataacaacc cagccgccag cgcatcgact ttacgctctt ctgccagcgc
     7381 catgccaata aagaacggtg ccattaaaga cattatccct aatgttccgt tatatacgtt
     7441 gccgccaata cctttcagac cattaagtgt ttcaatggtt gaggcgtcga ggcgaatacc
     7501 taaggaataa aaaaacgacc cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa
     7561 catggccccc gcaagggtta acggcattaa gcgaataaag ccatttttga ttgcattaac
     7621 gtgtggctgc tttcctattt taactgcaaa agggaggagt accttttcaa gcgatgcaat
     7681 aacattactc atagaaaaat acccttaaaa accgcaattt aaatattgcg gtattgattt
     7741 atgaaataac tctttgacgg gaaaatttaa aataaattaa tttgctgcgg cttttttaat
     7801 cgctgcaacc gcagccttaa gcacgcctaa accatcgact ttgccataaa gcagcgagtc
     7861 aattacttca accggtttgt tgggtaacaa acgctggatt tcgggcaaca tataagcaat
     7921 ctgcggccct aataacacga catcggcatt ctgacctttt tcaccagcca gtgtttccgg
     7981 aaatgcttca ataatgaccg gaacttcata tttttctgcc tgtgcgcgca tttttgatac
     8041 cagtaaagag gtagacatgc ccgcagaaca aaacagataa atgtgtttct tttccataaa
     8101 actgccctca tcgacgatta tctgtcagcc agacactccg caagccttaa cctgcttcca
     8161 tgctctgggt aacttgcgaa accaacatga ctttttttgt ggctgaaagg agtatacggt
     8221 atagacctga gcaatagtat ttctttgacc ttataaattg tctctccttg acctgcggtt
     8281 atgaccctct tcacatttcg ggcaaataat tcgcgaagat aattaagaac tcaggccaaa
     8341 aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag ctttcagacg aagtgattac
     8401 ttcgcagcct gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca
     8461 tgtccggtat catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg
     8521 atgtaggtct ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc
     8581 gcaatttgtg caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt
     8641 acaaactggt ctttggtacg atcataagcc gtacaacccg ccagcatggt taataccgcc
     8701 gctgcactca gaattcctgc catattcttg ttcatattcc gtcctattgt ttatcagcgt
     8761 gttagataag cctggaatac attgggcgct ttttcaagcc catgaacgaa acggctccgc
     8821 tttcagagga ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt
     8881 cagtagcaac gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga
     8941 cattgcaaca acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg
     9001 aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat
     9061 cactggtgct cgggatcagc ggcggtcagg actccacgct tgccggtaag ctgtgccaga
     9121 tggcgattaa tgagctgcgc caggaaaccg gcaacgaatc actgcaattt attgccgtac
     9181 gcctgcccta tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc
     9241 aaccggatcg cgtattaacc gttaatatca agggcgcggt attggctagc gagcaggcat
     9301 tgcgggaagc atgcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc
     9361 ggatgaaagc acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc
     9421 atgcagcaga agccattacc ggattcttca ctaaatatgg tgacggcggt acggacatta
     9481 atccactgta tcgtctcaac aaacgtcagg gtaaacagtt actggcagca ttaggttgcc
     9541 cggaacacct ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc
     9601 cagatgaagt ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa
     9661 acgtacctca acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac
     9721 gccgtccgcc aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacctattg
     9781 gattgtgcaa aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc
     9841 tacaaaacta tgcaaattca atacattgca ggagtcgaat aggcctgaca ggcgtagcac
     9901 gtcagacggt gtaacctttg tcatcgaccc gcctcttttt taatcgcttc ccggctgtta
     9961 cactggatag ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg
    10021 ctcgaatttg aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat
    10081 gacttaccca cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc
    10141 tatatcggca aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat
    10201 gaagccgcca tgctacggca atcccggcga attagctgga tctgcaccgc aggcgaaatc
    10261 ggcgctctgc tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg
    10321 ttgcgccgca atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg
    10381 tatgccaaag aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg
    10441 cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac aaaaactttg ttatggtctg
    10501 ttgggactgg aaccgttaag tcgcggtcgt gcgtgttttc gttcagcgct aaaacgttgc
    10561 gccggagcat gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct
    10621 ctggagcgtt tgcgggtggt gtgttggcct tggcaagggg cggtggcgct gaaagaacag
    10681 cacccggaaa tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat
    10741 tcgctggaag aagcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat
    10801 aaaattcttt gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg
    10861 aatgaccagc gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc
    10921 gcagggtgtg cctgccatcg aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc
    10981 ccagttgcca cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa
    11041 acgtacgttc ggcaattctg actttcgcct tacagacatc caggcgcgtc ataatattga
    11101 aatccatcga catctgccct gccgtcagtt tcgcttttac cacctggtcc gccgcaaagg
    11161 caaaaggctg taacggtttt aaggtatggt taaagtggtc tgcgctttca aggaacatct
    11221 cgccgccttc cagcaacgtc actatccttt ccataccagg aaacaaagaa aactccccat
    11281 tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag
    11341 tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacaa attcaccgac attttacgca
    11401 tatcaaagta ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac
    11461 cgtggatggt tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca
    11521 actgtttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt
    11581 ggacaagacc ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt
    11641 ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt
    11701 ttctgttccg gcgtcaggat gttgtaaatt ttgttctggg tttccatgtg cgccagcatg
    11761 ttagctttgc gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag
    11821 gtatcgctgg caatgatgtc atgcattgcg cgacgttctt ccagcggcgg acgtttcatc
    11881 tggtcacgct ggcctttcat gatttcgcga atctgctgtt tctgcgcgtc ggtcaggttc
    11941 aggtctttga acatcatgtc ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc
    12001 ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat gggccaggtt agccgcgcca
    12061 agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct
    12121 ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacgagat taactttact
    12181 gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt
    12241 gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa tattacaaca
    12301 ccatgatttt gcagagatta tgaagaacta taccggatg
//
LOCUS       DAZFKY010000142        12215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFKY010000142 DAZFKY010000000
VERSION     DAZFKY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            77..946
                     /gene="yjeJ"
                     /locus_tag="ACHKCL_003507"
     CDS             77..946
                     /gene="yjeJ"
                     /locus_tag="ACHKCL_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5764400.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1139..1492)
                     /gene="yjeI"
                     /locus_tag="ACHKCL_003508"
     CDS             complement(1139..1492)
                     /gene="yjeI"
                     /locus_tag="ACHKCL_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5764401.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1630..3276)
                     /gene="groL"
                     /locus_tag="ACHKCL_003509"
     CDS             complement(1630..3276)
                     /gene="groL"
                     /locus_tag="ACHKCL_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5764402.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3320..3613)
                     /gene="groS"
                     /locus_tag="ACHKCL_003510"
     CDS             complement(3320..3613)
                     /gene="groS"
                     /locus_tag="ACHKCL_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5764403.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3889..5145
                     /gene="yjeH"
                     /locus_tag="ACHKCL_003511"
     CDS             3889..5145
                     /gene="yjeH"
                     /locus_tag="ACHKCL_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5764404.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5161..5637)
                     /gene="fxsA"
                     /locus_tag="ACHKCL_003512"
     CDS             complement(5161..5637)
                     /gene="fxsA"
                     /locus_tag="ACHKCL_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5764405.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5974..7410
                     /gene="aspA"
                     /locus_tag="ACHKCL_003513"
     CDS             5974..7410
                     /gene="aspA"
                     /locus_tag="ACHKCL_003513"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5764406.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7528..8829
                     /gene="dcuA"
                     /locus_tag="ACHKCL_003514"
     CDS             7528..8829
                     /gene="dcuA"
                     /locus_tag="ACHKCL_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5764407.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8945..9283
                     /gene="cutA"
                     /locus_tag="ACHKCL_003515"
     CDS             8945..9283
                     /gene="cutA"
                     /locus_tag="ACHKCL_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5764408.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9259..10956
                     /gene="dsbD"
                     /locus_tag="ACHKCL_003516"
     CDS             9259..10956
                     /gene="dsbD"
                     /locus_tag="ACHKCL_003516"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5764409.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10993..11568
                     /gene="yjdC"
                     /locus_tag="ACHKCL_003517"
     CDS             10993..11568
                     /gene="yjdC"
                     /locus_tag="ACHKCL_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5764410.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11675..11750
                     /locus_tag="ACHKCL_003518"
     tRNA            11675..11750
                     /locus_tag="ACHKCL_003518"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11708..11710,aa:Phe,seq:gaa)
     gene            11948..12107
                     /locus_tag="ACHKCL_003519"
                     /pseudo
     CDS             11948..12107
                     /locus_tag="ACHKCL_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2947 a         3151 c         2996 g         3121 t
ORIGIN      
        1 ttactaccaa gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg
       61 gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa
      121 gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt
      181 gttctttatg tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca
      241 tcaaaagcat aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt
      301 cattaagaaa atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga
      361 tattaatcgt cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt
      421 cgtattaacg ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga
      481 aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt
      541 acgtattacc tcactgttag atttcctgcc gttatatgac gttgactgcc agcaaaatgg
      601 caatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta
      661 tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt
      721 ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct
      781 tgatttcagc ccacgactga aaaaactcgt gggtgtgcca tgtcaggtct atgttcgcac
      841 cgtagcggcg aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca
      901 cttacgtgtt caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta
      961 atgcgaggct ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg
     1021 atgcggcgta aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc
     1081 cttatgtagg cctgataagc gaagcgcatc tggcaattat acgtttgtca ccatcacatc
     1141 agttcgggca cttatatacc tggccgataa tctggctatc cgtcgggaca aaactggaca
     1201 acattccctg cgacgggcta ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1261 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1321 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1381 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1441 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1501 ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag
     1561 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaggt
     1621 gcagggcaat tacatcatgc cgcccatgcc acccatgcca cccatgccgc cagcagcgcc
     1681 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1741 gccagccaca gaagccgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1801 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1861 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1921 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     1981 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2041 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcgtcttcaa cgcgtgcttt
     2101 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2161 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2221 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcgccca cgccatcgat
     2281 gatggtggtg gtgtctttgt tgatcacaac gcgtttagcc tgacccaggt cttccagggt
     2341 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2401 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ctgcagcaac
     2461 tttcacgatg ccacgcatgg tgttaacaac cagagttgcc agcgcttcgc cttctacatc
     2521 ttcagcgatg atcagcagcg gtttgcctgc tttggcaacg gcttccagaa ccggcagcat
     2581 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2641 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2701 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2761 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2821 agcggagata gtaccaacct gagcaatcgc tttagagtca gagcacggta cggacagcgc
     2881 tttcagttct tcaactgcag cggtaacagc tttgtcgata ccacgtttca ggtccatcgg
     2941 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3001 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgcttta gaggcaactt ctttcaccat
     3061 ctgcgcaccc atgttttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3121 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggcctttcgg
     3181 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3241 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3301 gttcagtgtc gtgcgaggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3361 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3421 cgatgtcgcc aactttcaca tccagcggct tcacttcgcc attttcaagg atacggccat
     3481 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3541 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3601 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3661 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3721 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3781 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3841 aaaatctacg cagttgccca aaatttgggc gcaatcggac atcaacccat gagtggactc
     3901 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     3961 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcgg gcaataacag cctgtgggcg
     4021 tggcccattt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4081 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4141 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcgcta
     4201 caaattgctg ccggattcgg ccaggcaatg tttggctggc atagctggca actgttgttg
     4261 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctcgcggtgc cagttccagt
     4321 gcaaatctac aaacagttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4381 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4441 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4501 catcttgcct cggaatttaa aaatccagaa cgtgattttc ctcgtgcttt gatgattggt
     4561 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4621 tatggtgaac aaatggccgc ggcagcatcg cttccaaaaa ttgtagtaca actgttcggt
     4681 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtctcaac
     4741 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4801 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgctcggc
     4861 tgctgcgtgg tgagcacgtt ggtgattcat gctttagaga tcaatctgga cgctcttatt
     4921 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     4981 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5041 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggcggg gttatggctg
     5101 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5161 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctggta
     5221 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5281 agaaaagcgc aaatgcggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5341 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5401 gatcagcgaa acacttttaa tcatctccgc cgctgggttt tcacccgccg ccattttttg
     5461 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5521 cgtaaatata accagcacga gagtcagcaa tacccccaat acatgggcaa cctgaataaa
     5581 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5641 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc
     5701 agcttatatt gtggtcatta gcaaaatttc aagatatttg cgcaactatt tttggtagta
     5761 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5821 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcggata ttcggtcgat
     5881 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     5941 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6001 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6061 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6121 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
     6181 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6241 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6301 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6361 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6421 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6481 ttgcgtgaag gctttgaacg taaagcggtc gaattccagg acatcctgaa aatgggtcgt
     6541 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6601 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6661 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6721 gtgaaaaaac tggctgaagt tactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6781 gcgacctctg actgcggcgc ttatgttatg gttcacggag cgctgaaacg cctggctgtg
     6841 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6901 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     6961 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7021 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggagcc ggtcattggc
     7081 caggctatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7141 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7201 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7261 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7321 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7381 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7441 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7501 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg
     7561 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7621 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7681 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7741 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7801 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7861 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctgtct
     7921 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     7981 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8041 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8101 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggcct ggttgaactg
     8161 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8221 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8281 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag tgttgcaact
     8341 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8401 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8461 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8521 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8581 aaagcactga tgccgatggc tctggcactg aacgtttctc cgctgaccgc tgttgcttct
     8641 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8701 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8761 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8821 atgctgtaat gaccaatcgc ggggcgttca cgccccgctt tctttctcgc cgactaacat
     8881 cctttccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     8941 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9001 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9061 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9121 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9181 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9241 agacacagat tacctctcat ggctcaacgc atctttacgc tgatcctgct actttgcagc
     9301 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgtggat
     9361 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9421 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9481 gccgacgtgc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9541 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9601 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9661 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgttccg
     9721 cagcaagagc agcccaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9781 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9841 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9901 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
     9961 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcaccatcgt ctttaccttg
    10021 ctggcgatgt caatgtttgg cttgcttact ctacaactcc cctcctcgct gcaaacgcgc
    10081 ctcacgctga tgagcaatcg ccaacagggc ggctcacccg gcggagtgtt tattatgggg
    10141 acgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10201 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10261 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10321 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10381 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10441 gtcgcattct ttggctgggc ctttatcacc agcctacagg ccaaacgcgg ctggatgcgc
    10501 gtggtgcaaa taatcctgct tgcagcggca ttggttagcg tgcgcccact tcaggattgg
    10561 gcatttggtg cgacgcatac cgcgcaaact cagacgcatc tcaactttac acaaatcaaa
    10621 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10681 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10741 caggtgcaaa aagcgttagc agacacggta ttacttcagg ccaacgtcac tgccaacgac
    10801 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10861 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10921 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    10981 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11041 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11101 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11161 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11221 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11281 ggctgtctgt ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11341 ctggccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg
    11401 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11461 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ccgcacatcg gctggcggaa
    11521 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcac tgacctgaca gaaacacaga
    11581 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11641 gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11701 ggggattgaa aatccccgtg tccttggttc aattccgagt ccgggcacca aattcatata
    11761 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gtgttctgcc
    11821 tccaaatcaa ttctaccgaa ctcaacccga ttctccccac atcaccagca atttgcgggc
    11881 atatcccaat tcgggaaaat ttgttcctga gctatagcac taactaacgt gaaatgtcgt
    11941 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gcatccataa
    12001 agcgcaacat cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12061 gtatcagtcc gcttgctcat gcaaagacta acaatccatt aaaatagtaa gcgctccgga
    12121 caattttcca tggattattt tctgaacatt tttctttggc aaagatgatg aattttgatg
    12181 gtaaggaata ttacttctgg ttctcagtaa aatcc
//
LOCUS       DAZFKY010000143        12042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFKY010000143 DAZFKY010000000
VERSION     DAZFKY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..600
                     /gene="yebB"
                     /locus_tag="ACHKCL_003520"
     CDS             <1..600
                     /gene="yebB"
                     /locus_tag="ACHKCL_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5764411.1"
                     /translation="NINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHKCL_003521"
     CDS             complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHKCL_003521"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5764412.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHKCL_003522"
     CDS             complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHKCL_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5764413.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHKCL_003523"
     CDS             complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHKCL_003523"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5764414.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2497..4269)
                     /gene="aspS"
                     /locus_tag="ACHKCL_003524"
     CDS             complement(2497..4269)
                     /gene="aspS"
                     /locus_tag="ACHKCL_003524"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5764415.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALCVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4579..5145
                     /gene="yecD"
                     /locus_tag="ACHKCL_003525"
     CDS             4579..5145
                     /gene="yecD"
                     /locus_tag="ACHKCL_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5764416.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5142..5960
                     /gene="yecE"
                     /locus_tag="ACHKCL_003526"
     CDS             5142..5960
                     /gene="yecE"
                     /locus_tag="ACHKCL_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5764417.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6013..6408
                     /gene="yecN"
                     /locus_tag="ACHKCL_003527"
     CDS             6013..6408
                     /gene="yecN"
                     /locus_tag="ACHKCL_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5764418.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6449..7192
                     /gene="cmoA"
                     /locus_tag="ACHKCL_003528"
     CDS             6449..7192
                     /gene="cmoA"
                     /locus_tag="ACHKCL_003528"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5764419.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7189..8160
                     /gene="cmoB"
                     /locus_tag="ACHKCL_003529"
     CDS             7189..8160
                     /gene="cmoB"
                     /locus_tag="ACHKCL_003529"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5764420.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8325..10754)
                     /gene="torZ"
                     /locus_tag="ACHKCL_003530"
     CDS             complement(8325..10754)
                     /gene="torZ"
                     /locus_tag="ACHKCL_003530"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5764421.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10779..11879)
                     /gene="torY"
                     /locus_tag="ACHKCL_003531"
     CDS             complement(10779..11879)
                     /gene="torY"
                     /locus_tag="ACHKCL_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5764422.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2757 a         3190 c         2934 g         3161 t
ORIGIN      
        1 aatattaatt accctgctga atatgaaatt ggagataaag tctttacatg tataggtgct
       61 gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt
      121 atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaaccatc
      181 accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat aaagcgatta
      241 gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta
      301 cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt
      361 gtttttgata tttataaaga agcgctatgt attccggtgg gtgaaataga gacgtttgga
      421 gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcctgggt
      481 tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccaggtttg
      541 gtgttgattc atgcggaggg agtggaaacg cctcagccgg aactgaccga ggcggtataa
      601 cttaacgcag tcgccctctc gccaggttca gccgcgattc gctcatctgc atcgcattct
      661 gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc gcctgtggat
      721 tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcggcactac
      781 caatacctac caccgtttgc tttacctgac gtgccgcgta ttcaaatact ggcaactcct
      841 gattcaccgc cgccacaatc gccacgccgc gcgcctggcc cagtttcagg gctgagtcag
      901 cgttctttgc cataaagact tgttcaatgg cgaaataatc gggctggaac tgggtgatga
      961 tttccgtcac gcccgcatag atgagtttca gacgagacgg taaatcatcc actttggtgc
     1021 gtatgcatcc gctacccagg taggacagtt gcctacctac ctggcggatg acgccgtagc
     1081 cggtcacgcg cgaacccgga tcaatgccga gaataatagc catcacgcgt ctccgttttg
     1141 ctgtttagca ggcctcatca gagagtcgct gcgacctcat cagagatttc accgttatgg
     1201 taaacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt
     1261 gcggtttctg catccatatc agctttggta gacgggatca tggaaacttc cgcgctgtct
     1321 gctttcagac ctgccgcttc cagagcgtcg cgcactttac ccatttcttc ccatgcagtg
     1381 tagacatcaa tcgcgccgtc atcataggtc acaacgtctt cagcacctgc ttccagtgct
     1441 gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tgacgccttt tttgctgaac
     1501 agataggcta cggaaccatc agtaccgagg ttaccgccac atttgctgaa tgcatgacgc
     1561 acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg
     1621 ccaggaccgt aaccttcgta gatgatggtt tccatgttcg catcatcatc accgcccaca
     1681 ccacgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacag tgctttatca
     1741 attgctgcac gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt agccgcggtt
     1801 accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta
     1861 cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc agatagccct
     1921 gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt
     1981 agtgagcgcc gccgcagcag gcgcatcaag ccacttgtaa gccagatgtt cagtgaaaac
     2041 gatctgccgc tcatgcggaa gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc
     2101 cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc
     2161 aattaaggtc agttgttcag cgacaacatc aatggtgacc tcttccttta cttcgcgcat
     2221 ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc
     2281 ggggtcatca cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt agatgaccac
     2341 taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc gttgactgcc
     2401 ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctc
     2461 gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctcag ccttcttcac
     2521 aacctgaatg ctcagctcag ccagtgcagc cgggttagca aagctcggtg cttcagtcat
     2581 cagacacgct gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
     2641 ggtcagcagc atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc
     2701 gtatttcaga gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
     2761 cagaatacca aacaccgtct gctgcatatc accattatgg atacgtactg aaccaccgcc
     2821 cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt ccggtgcagc
     2881 tttcagctct gcagccgtca tgtctttcgg tgaggtgaac ggatggtgca ttgccgtcag
     2941 gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt
     3001 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc ccatcgcgtc
     3061 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt
     3121 acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
     3181 accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta
     3241 gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg cgccacccgg
     3301 aacacacaga gcggcaacgc gacctttcgg atcgttcgcc ggacctgcaa atactgcaaa
     3361 ctcaacagat ctcagcagat cagcaacgtc ggtcagttcc atcgggttac gcagatccgg
     3421 tttatcagaa ccataacggc gttcggcttc cgcaaaggtc attactggga aatcgcccag
     3481 atccacgccc ttcacttcca gccacagatg gcgcaccagc gcttccatca cttcacgcac
     3541 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc
     3601 agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtagcggt caaagccgga
     3661 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
     3721 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
     3781 cggagtttcg atgtcgagga aaccgtggtc atccataaaa cggcgcacca ggctggtgat
     3841 tttagcgcgg gttttcaggc gctgagccat ttccggacga cgcaggtcga ggtagcggta
     3901 tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcgcg
     3961 gttgatgata gtcagcgagg acgccagcac ttcgatttcg ccggtcgcca tatcgcggtt
     4021 aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg
     4081 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
     4141 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
     4201 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
     4261 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
     4321 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
     4381 aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat
     4441 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcatacaa
     4501 attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgtgag gcacgatttt
     4561 gttttttcag gagtcatcat gcttgaactt aatgctaaaa ccactgcgct ggtggtgatc
     4621 gatttacaag aaggcatctt accttttgcc ggtggtccac atactgccga tgaggtagtt
     4681 aatcgcgccg ggaagctggc ggcgaaattt cgcgccagcg gtcagcccgt gtttctggtg
     4741 cgcgttggct ggtctgccga ttacgccgaa gcattaaaac agccggttga tgccccttcc
     4801 cccgctaaag tgttgcccga aaactggtgg caacatccag ctgcattagg tgcaactgac
     4861 agtgatatcg aaataatcaa acgtcagtgg ggtgcgtttt acggtacgga tctggagttg
     4921 caattacgcc gccgaggtat cgatacaata gtgttatgtg ggatctcgac caatatcgga
     4981 gttgaatcca ccgcccgcaa tgcctgggaa cttggcttta atctggtgat tgccgaagat
     5041 gcctgtagtg ccgctagcgc cgagcagcac aataacagta tcaaccatat ctacccgcgc
     5101 atcgcccgtg tgcgtagcgt ggaagagatc ctcaacgcgt tatgatttac atcggtctac
     5161 cgcaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc
     5221 gtcactttaa ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg
     5281 tcctgcgctg gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga
     5341 ccatttcgca tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc
     5401 gcatgtcacc gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg
     5461 gcccacggga gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt
     5521 atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta
     5581 atcgcggttt acatcagcgc ggcgttaatc aggtgatttt agacagccgc ccggttcatg
     5641 cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg
     5701 tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga
     5761 cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca
     5821 ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa ctggtacata
     5881 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
     5941 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
     6001 aagggagttt gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag
     6061 ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt
     6121 tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc
     6181 gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
     6241 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
     6301 ttccgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
     6361 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
     6421 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
     6481 tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat
     6541 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
     6601 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
     6661 gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc
     6721 cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt
     6781 agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
     6841 gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcgttactgg ataaaattta
     6901 tcaagggctg aaccccggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc
     6961 caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag
     7021 cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt
     7081 ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca
     7141 gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt
     7201 ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc
     7261 gcgcagattg ctaactggca gcgcgagcag cagcacgggt tgtttaagca gtggtcaaat
     7321 gcggtggagt ttctacctga aattaaaccg tatcgtcttg atttattgca tagcgttacg
     7381 gcagaaagcg aagagccact gagcgcaggg caaattaaac gcattgaaac gctgatgcgc
     7441 aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
     7501 tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc
     7561 accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc
     7621 gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg
     7681 cgtaaactac tgggtaatga tcagcgcgcg catttgttac cgttaggtat tgaacaactt
     7741 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca
     7801 ccgctggagc atctctggca gttgaaagat caactggtca atgaaggtga actggtgctt
     7861 gaaacgctgg ttattgatgg cgatgagaac acggtgctgg tgccgggcga tcgttacgcg
     7921 caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag
     7981 tgtggttttg tagatatccg cattgcggat gtgagcgtta ccactacaga agagcagcga
     8041 cgcactgaat ggatggttac cgagtctctg tccgatttcc tcgacccaca tgatccgagt
     8101 aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa
     8161 aggtctggta ttactgccgg atgcggcgtg aacgccttat ccagcctaca aaaccttgct
     8221 aattcaatat attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagcttta
     8281 tgtttgcata acctcagcgc ccgtttccgg gcgcttttca cgtcttatgc ctgaacagct
     8341 ggctgatcaa acgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc
     8401 gcagagttac cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg
     8461 ttcgcactgc cgtttttaca caagccattt tccagatctg gccatgcacc ttcatgcacg
     8521 cataccacgc cttttttgat cccgtcagtc accacagcgc ctgtcaaaat ctgaccacgt
     8581 ttgttccaga cacggaccag atcgccattc gcaataccaa agcgagcagc atcttcggtg
     8641 tgaatagtga ttggttcacg atcggcaacc gcatattttt tacgcagttg cgcatagtta
     8701 agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgtaactg cttctcgtcg
     8761 gcggtacctt tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag
     8821 ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca
     8881 tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg
     8941 cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg
     9001 cgttgcgcac gggcaccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca
     9061 tctttacctt cggtatagat ctcttttccg cctggtttca gtaattccgc aagttcggca
     9121 aacacgtcaa aatcgtttcg cgcttcaaat tgcggagcga cagcctgctt catcggtaca
     9181 atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg
     9241 atcggtaata cgatatctgc atgtttagca gctgcggtcc agtagcattc agaaacgacg
     9301 atcatctccg gtttctgcca tgctttgatc agacgattag tatcctggtg atgggtaaag
     9361 ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta
     9421 tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc
     9481 gtcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc
     9541 ggcagcacgc cgccaacacg cgtcgggttg ccaccgttgg agtaatgata agagaaacca
     9601 aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag
     9661 tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag cattgtgcga
     9721 ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc
     9781 gccgtccagg ccgcgctctt cggagtatta tcgctcttac ctgtcagata ctcttcaaac
     9841 tgcggatacc cggtggtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc
     9901 agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag
     9961 gtggcattat agccaaagaa ctcgatggtt tcagagcgga taggatcaat ggcaatcact
    10021 ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc
    10081 caggcaattt ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt
    10141 tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc gaccacatgc
    10201 ggcatgatca cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc
    10261 gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc
    10321 cagccgtaag atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt
    10381 tgctcatgaa tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca
    10441 cgacctttcg ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga
    10501 atacgcgccg tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt
    10561 ttcgccagcg cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gttcatcgct
    10621 ccccaacgtc ccgcggtgag gattttaccg cccttctctt ctgcccatgc gggtagcggt
    10681 gctgccgatg tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt
    10741 cttgttaatg tcataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct
    10801 ttggcgttgt actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt
    10861 cgtgccccca ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca
    10921 ataggggcat gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc
    10981 agtttacggt ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat
    11041 tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct
    11101 tcacttaatg tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg
    11161 ccctgcattt gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg
    11221 gtgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga
    11281 taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg
    11341 ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag
    11401 gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca
    11461 atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat
    11521 tctttccata ctgtttcggc catttcctgg cgatgagctt cgaacttatc gtcactgtct
    11581 attttgccgc taacaaattc atgataaata tctttagatg cttttaattt agcgaataaa
    11641 taatccatcc ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg
    11701 ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa
    11761 aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccatcaa gaacccgcca
    11821 ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata
    11881 gattcctcta attaaattcc agaacaccga aatcattgta taaataaata cacaacgaat
    11941 tgactacttg cttaattcgg cacaacactg cctgaaataa tcgctaaaga atatgattta
    12001 ttacaatgta atcattaatt gctaaggaat aatccagttg tt
//
LOCUS       DAZFKY010000144        12019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFKY010000144 DAZFKY010000000
VERSION     DAZFKY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..635
                     /locus_tag="ACHKCL_003532"
     CDS             3..635
                     /locus_tag="ACHKCL_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764423.1"
                     /translation="MARAGEYKNYSRDEIKYWRYTSYKGGKLPEGFTDEKFSSAIYNG
                     RIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYELYEG
                     DATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTCGWD
                     FIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
     gene            651..1061
                     /locus_tag="ACHKCL_003533"
     CDS             651..1061
                     /locus_tag="ACHKCL_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764424.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1282..2103
                     /locus_tag="ACHKCL_003534"
     CDS             1282..2103
                     /locus_tag="ACHKCL_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5764425.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2185..2664
                     /locus_tag="ACHKCL_003535"
     CDS             2185..2664
                     /locus_tag="ACHKCL_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5764426.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2679..3155
                     /gene="yeeS"
                     /locus_tag="ACHKCL_003536"
     CDS             2679..3155
                     /gene="yeeS"
                     /locus_tag="ACHKCL_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5764427.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3218..3439
                     /gene="yeeT"
                     /locus_tag="ACHKCL_003537"
     CDS             3218..3439
                     /gene="yeeT"
                     /locus_tag="ACHKCL_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5764428.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3513..3881
                     /gene="cbeA"
                     /locus_tag="ACHKCL_003538"
     CDS             3513..3881
                     /gene="cbeA"
                     /locus_tag="ACHKCL_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO5764429.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3970..4343
                     /gene="cbtA"
                     /locus_tag="ACHKCL_003539"
                     /pseudo
     CDS             3970..4343
                     /gene="cbtA"
                     /locus_tag="ACHKCL_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4340..4534
                     /gene="yeeW"
                     /locus_tag="ACHKCL_003540"
     CDS             4340..4534
                     /gene="yeeW"
                     /locus_tag="ACHKCL_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5764430.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4580..4660
                     /locus_tag="ACHKCL_003541"
                     /pseudo
     CDS             4580..4660
                     /locus_tag="ACHKCL_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4949..5095)
                     /locus_tag="ACHKCL_003542"
                     /pseudo
     CDS             complement(4949..5095)
                     /locus_tag="ACHKCL_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5149..5331
                     /locus_tag="ACHKCL_003543"
     CDS             5149..5331
                     /locus_tag="ACHKCL_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5764431.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5432..5761)
                     /gene="tmaR"
                     /locus_tag="ACHKCL_003544"
     CDS             complement(5432..5761)
                     /gene="tmaR"
                     /locus_tag="ACHKCL_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5764432.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5933..6991)
                     /gene="yeeA"
                     /locus_tag="ACHKCL_003545"
     CDS             complement(5933..6991)
                     /gene="yeeA"
                     /locus_tag="ACHKCL_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5764433.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7190..7663)
                     /gene="sbmC"
                     /locus_tag="ACHKCL_003546"
     CDS             complement(7190..7663)
                     /gene="sbmC"
                     /locus_tag="ACHKCL_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5764434.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7782..8948)
                     /gene="dacD"
                     /locus_tag="ACHKCL_003547"
     CDS             complement(7782..8948)
                     /gene="dacD"
                     /locus_tag="ACHKCL_003547"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5764435.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            9157..10584
                     /gene="sbcB"
                     /locus_tag="ACHKCL_003548"
     CDS             9157..10584
                     /gene="sbcB"
                     /locus_tag="ACHKCL_003548"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5764436.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10627..10854)
                     /gene="tsuB"
                     /locus_tag="ACHKCL_003549"
     CDS             complement(10627..10854)
                     /gene="tsuB"
                     /locus_tag="ACHKCL_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5764437.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10868..11926)
                     /gene="tsuA"
                     /locus_tag="ACHKCL_003550"
     CDS             complement(10868..11926)
                     /gene="tsuA"
                     /locus_tag="ACHKCL_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5764438.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         3164 a         3115 c         2862 g         2878 t
ORIGIN      
        1 ttattgcacg tgccggggaa tataaaaatt acagccggga tgaaataaaa tactggcgat
       61 atacatcata caaggggggg aaacttccgg aaggtttcac tgatgagaaa ttttccagcg
      121 ccatttacaa cggaagaata tttacaatga aacgtttaca tacccttatg ttatttctgg
      181 cggttctgtt cacgggcttt aactttaacg cagaagcggc taccgtaaaa caagctctta
      241 gttgtaaccc ggaagcctgg gctgaacaac ctggagcgtg tccatcaacg tacgagttgt
      301 acgaaggtga cgctacctac aaagccgcta tcgacaaagc attaaaaccg gtcggactga
      361 gcggcatgtt cggtaaaggc ggctatatgg atggccctgg tggaggtatc acgccagtaa
      421 acattaacgg tacagtctgg ttccagggcg acggttgcaa agccaatacc tgcggctggg
      481 actttatcgt aacactctat aacccaaaaa cccatgaagt tgttggctac cgttactttg
      541 gtttagatga cccggcctac ctggtttggt tcggtgaaat tggcgtgcat gaattcgcgt
      601 atctggtgaa aaactacgta gctgcggtta actaatttaa ggagtctatc atgaaaactt
      661 ttatcaaaac tttactcgtt gctgtaacta ttctgttctc tgtcttcgct actgcgaaac
      721 aagtaaaact gccaaataac atcaaatacg ttaatactac agaagcgttt tcctgtaccg
      781 aaattgacgg tatgaattgc cagacaaaga atcagttcaa ctataaagat aacagctatg
      841 ttttcgtgct tgaacgtggt ggtgcctggt gctacgacta cactgtctcg gtagttaacc
      901 tgaaaaccgg gaaagcacaa atgattgaat acggggacaa tcaactgtgc tcaggtagca
      961 acaaaccgtt cttcgaaatc aaaaatggcg taccgacggt aggagtcatc gacacatccg
     1021 gaaaacctgt cggtgtagcc caggacaaac ttaaaatcta aggagtaaac catgcgtttc
     1081 tctgcacatt attaatatca aaggtaaata cagccccagc cattgttacc ggaaagccgc
     1141 ttaatgcgtg gctcgccagt gaaaggcctc tcagacaata aaccttattc atttcacccg
     1201 tcaggccgcg tcttctccgg gagacgcggc ttttttcatt tattgcacca gtaaatctta
     1261 accaccgata aggagcaaag tatgcgatta gccagtcgtt ttggttatgc tgcaaaccag
     1321 atacgccgtg accgtccgct gacacatgaa gaactgatac gccatgtacc cagtattttt
     1381 ggggaagacc ggcacacctc ccgcagtgaa cggtatgcgt acattcccac cattaccgtc
     1441 ctggaaaatc tgcagcggga aggctttcag ccgttcttcg cctgccagac ccgtgtgcgc
     1501 gacccgggcc gccggggata cacaaaacac atgctgcgtc tgcggcgggc cggagagata
     1561 aacggagaac atgtccctga aattattctg ctcaactctc atgacggtac ctccagctac
     1621 cagatgctgc cgggttactt caggttcgtc tgccagaacg ggtgcgtctg tggccagtct
     1681 ctgggggaag tgcgtgttcc acaccgggga aatgtagtgg agaaagttat cgaaggggct
     1741 tacgaggtgg tgggcgtgtt tgaccggata gaggagaagc gtgatgccat gcagtcgctg
     1801 gtcctgccgc aaccggcacg ccaggcgctg gcacaggcgg cactgactta ccgttatggt
     1861 gacgaacatc agcccgtcac caccgccgac attctgacgc cgcgacgccg ggaggattac
     1921 ggtaaggacc tgtggagcgc atatcagacc atccaggaga atatgctgaa aggcgggatt
     1981 tccggtcgca gtgcaaaagg aaaacgtatc catacccggg ccattcacag cattgatacc
     2041 gacattaagc tcaatcgcgc attgtgggtg atggcagaaa cgatgctgga gagcctgcgc
     2101 tgatgccgtt tccctgagtg agggaatcat tcagctttcg ttcccgaaaa acacacattt
     2161 atttgttatt taaggagtta tctcatgaaa accgtttctc agaatacccc cacaatttat
     2221 tcagctacaa caccagagaa taatccgcct cagttggttg ccagcctcgt ccctgatgaa
     2281 cagcgcatca gcttctggcc gcagcatttt ggcctcattc cacagtgggt caccctggag
     2341 ccccgtgtct tcggctggat ggaccgtctg tgcgaagact actgcggtgg tatctggaat
     2401 ctgtacaccc tgaacaacgg cggggcattt atggcccccg aaccggatga tgatgatgac
     2461 gaaacatggg tactgttcaa tgccatgaac ggtaaccgcg ctgaaatgag cccggaagcc
     2521 gccggtattg ccgcctgtct gatgacgtac agccatcatg cctgtcgtac ggagaattat
     2581 gccatgacgg tccattatta ccggttgcgg gattacgccc tgcagcatcc ggaaggcagc
     2641 gccattatgc gcatcattga ctgaaagggg ccggaataat gcaacagatt tcctttctgc
     2701 ccggagaaat gacgcccggc gagcgcagcc tcattcaacg ggccctgaaa accctggacc
     2761 gccatcttca tgaacccggt gtggccttca cctccacccg tgcagcacgg gaatggctga
     2821 ttctgaacat ggcgggactg gagcgtgaag agttccgggt gctgtatctg aacaaccaga
     2881 atcagctgat tgccggtgaa accctcttca ccggcaccat caaccgcacg gaagtccatc
     2941 cccgggaagt gattaaacgc gccctgtacc acaatgccgc tgccgtggta ctggcacaca
     3001 atcacccgtc cggtgaagtc acacccagta aggcagaccg gcttatcacc gaacgtctgg
     3061 tacaggcact gggcctggtg gatatccggg tgccggacca tctgatagtc ggtggcaacc
     3121 aggttttctc ctttgccgaa catggtctgc tttaacccgt cacaaccaca tcacacctgt
     3181 tttcactttt atcttctgtc ttcagaggta tcccattatg aaaattatca cccgtggtga
     3241 agccatgcgt attcaccaac aacatccgac atcccgtctt tttccgttct gtaccggtaa
     3301 ataccgctgg cacggcagtg ctgaagcgta taccggtcgt gaagtgcagg atattcccgg
     3361 tgtgctggcc gtgtttgctg aacgccgtaa ggacagtttt ggtccgtatg tccggctgat
     3421 gagcgtcacc ctgaactgag tgggaattct gatgagcaga attatcgcca ctaccggacc
     3481 attcttagcc gattttctgt aaggatttta tcgtgtcaga cacactcccc gggacaacac
     3541 ttcccgacga caatcacgac cgcccctggt gggggctgcc ctgcaccgtg acgccctgtt
     3601 tcggggcacg tctggtgcag gagggtaacc ggttgcatta ccttgccgac cgcgccggta
     3661 tcagaggcct gttcagcgat gcagatgcgt accacccgga ccaggccttt ccgctgctga
     3721 tgaaacaact ggaactcatg ctcaccagcg gtgaactgaa tccccgccat cagcataccg
     3781 tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcagcagt tgtggttacg
     3841 tttatctggc tgtttatccg acgcccgaaa tgaaaaatta actctccaga atagccttct
     3901 gctacggcct ggtgttttca ccacgccact tttccatttt tatatctgca tatcaggaaa
     3961 atcttcagta tgaaaacatt acctgtatta cccgggcagg cggccagttc tcgcccgtct
     4021 cctgttgaaa tctggcagat actgctgtcc cgactgctgg accagcacta tggcctcaca
     4081 ctgaatgaca caccttttgc cgatgaacgt gtgattgagc agcatattga ggcaggcatt
     4141 tcactgtgtg atgcggtgaa ctttctcgtg gaaaaatacg cgctggtgct accgaccagc
     4201 cgggattcag cgcctgtacc cgctctcagt taataaacag catcgatatc ctccgggctc
     4261 gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac attaccctgg
     4321 gtaagtatcc ggaggcgaaa tgatgacact ggaagccgac agcgttaacg tacaggcgct
     4381 ggatatgggg cacattgtcg ttgacattga tggtgttaat atcactgaac tgattaataa
     4441 ggccgctgaa aacggttatt cactccgcgt ggtggatgac cgtgactcta ccgaaacacc
     4501 ggcaacttat gccagccctc accagttgct gtaagacaat gcagtgatgc agggtatagt
     4561 gccattaacc acagaaacag cactgttgtc ctgtattaag caggcaagag aagatgccct
     4621 taccctgcgc catctgcagc ttctgcacca gaaccggtga atctcactca acaggcttca
     4681 tttcccctga cgccagcctg aatacagctg gcgttttcat ttataaacag aaaggaaaac
     4741 cgataataat ggaatggacg ccccgaccat gaagcaacgg taggatatta ctgataaacc
     4801 ttccagtgga ggtgcgttat ggccgtttta acgattagca ttgacctgac aaaaaatgaa
     4861 ttccaaatcc acggtctggg taggaacaga aaaatctaag ctcagaaaac ggattaaagt
     4921 aagcgtcaac ggagcaccgt attgacgctt atttattggt gagaactacg ttccatggca
     4981 ggagttcgtc aacacggttg gagggccatt ccggcagtat gctcagaata tggcgcagat
     5041 acgcttccgg atcgataccg ttcagacggc aggtgcctgc ttttccggca acaagcagga
     5101 atctcagcgc tgaagcagga attagaggta aaaacgccct accgggccat gaatcacccg
     5161 gtcattggag tagtaaccaa agcagattta gccagcatgg aacaaatatc tttggtgaaa
     5221 tgttggttac gggaagctgg agcgcacaac gtgttagtaa ccagtgcggt taataacaat
     5281 ggagttacag aactttttgc cttgctgcat acagaagaag gctgccgcta atttcgatag
     5341 gttctcagat gagaatgatg acggtgatac acagataaga atcaatgagc aatgagggtt
     5401 gccgggcaac cctcattgaa taaaacagga attacttcgc ttcgccgttt ttcatttcgc
     5461 ccatagcttt cagctttttg gagatatcgc ggcgttcttt ggagagctcg gcatttttga
     5521 tgatgtaatc gtcaacgcga tcttcatagt cacctttcat gctggcgatg atgccctgga
     5581 ttgcttcaac gctcatccct ggcttgatgt aatcgctcag gttgtccagc aacaggacgc
     5641 gcttctggtt gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc agtttgttct
     5701 tacgacggaa cagacgaaca aattccagta cgtcctggaa tgaaggcttg gtagtttcca
     5761 tttttatacc cctgataatg tgagagtcgg attcgtttaa gcaaccgcta ttcgttaggg
     5821 ccaacattac tcatagctgc cgcagatgac aatgctttta tcccttatca ctatcatacc
     5881 ctttatcctt gctgaatcga agcagcagca agatgattct gaagttcagg aattatttgc
     5941 gcaaggcccg gcaaatcaga ctcgatagca actcaagctg atgtgccgtt tccatgttca
     6001 gccacacata accatagatt ggtgtttcca caaccttcag gtcatggtgg ttattcagca
     6061 actgacgcag ctcttccaca gcatcgttca atttttccgt attggcaaaa accggctgcg
     6121 gattaccttc gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc atgtgctggg
     6181 tatcacgaag tttttgcgcg ttcaataaca cgaaatggct ggggcgcgtg gcccagtatg
     6241 cattgatttg caactccagc atacaaacca gattgcggtt aatggtctgg ataccttcat
     6301 atatcgattt tggaatacga gtttctttgc tggcgggcgc aatcagccca cgcattttca
     6361 cggcatcggt caggagtttt tgtagatggc tttccagacg tgggcgttcg agtaagttcg
     6421 gtgagaatgc agattgatag acccgattat actcggtcag acttttcgcc agttgaatgc
     6481 gccagtggat gaacgcccgt tgtggccaga taccggtaaa caacattgcc agtaaagagc
     6541 cgaggatcac atcgccgctt cgccataacg ccgtatcaat ttcacctgtc ggggaaccca
     6601 caacaattgc cagcgtcacc ccaatcaata atccttgata cggtttcttg cccagcgcca
     6661 gccaaccgca aagaaacatc gccgccgcgc accagactaa catcagcggt aacgaaatta
     6721 actccagttg cagagcgata agacctaaaa tcgaccccaa caccgtaccg ccaatacgtt
     6781 cgaaggcacg agggacaacg ttcccccaga acgagattgg ccccataatc accaccatgg
     6841 tgaccagcgg ccaggtgcct tccgggatag taaacaggcg gataatgaga aaagtgagca
     6901 ggaatgccag cgcgacccga gtaccatgca caatgcggta atggcggtat acccggattt
     6961 caaacgggct taatgactta ttggcacgca cgcagaaact ccgttaatga agattaaaag
     7021 cgctcataac acatctgtcg gtcagaaggc gcgcctctga attatccgac ggacaaaccg
     7081 ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa ggcgcatctt actcttttcg
     7141 ctttcaaaaa aagagtggtc ttcgcgtcaa cacaccgccc tgagatgaat tagtgatgtt
     7201 ttggctgcac cgcaacatac atttcgatat cccagtaccc atcttccgcg ccattgttca
     7261 agtaaacctc gaagcagggc tttggtaaca tttcataagc actgtcctgc aagaggctat
     7321 taaagaactg ataccagggt ttagcaaaat catcaccgac tacacgagcc accgccaccg
     7381 catattgacc acctgaaatt tctgtcagaa tgacgccctc actgttttcg gggagcgtaa
     7441 agttattcgg taccgtcacg acggtgtcgc agcgtaattt ttcggcgggt gtttcatccg
     7501 gattgtcgta atagacagca acccactcct tcggcacaat atttttgcta tctacccaca
     7561 tcatcaactg ctcaaaacct ttctttaccg tctgttccca cgggccaacg agatggaaac
     7621 ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt catgacgcct ccattattac
     7681 tgtttttata tacagtatag ttgcaatatt aaaaccacaa ggaatgagtg ttgattatgc
     7741 gagcagactc gcactcctgc cagtctgctg caaaagaaag gtcaggcctt atggtggaaa
     7801 taatcactca ggcgagaaaa catgctgcct tccccgacag attccagggt aaccagcggc
     7861 cagtgcgcca cctgtttatc acggtcgtaa agttcaattt cccctacccg ctgatgggcg
     7921 ctaattggcg cggtgagctc tttaccatca agggtatatt tggctttgat atgtggaatt
     7981 tcggctttcg gtagcaccat ccagaactct tgttccgttc ccagggcgat attttcttta
     8041 tcgccatacc agatgcgttc cgtaccgacc tttttcccac ggtgcaaaat ttgcaccgta
     8101 gtaaagtttt gttgccccca acgcagtaat tttcttgcct cttcctcacg accttttgca
     8161 ctgtcagccc ccattaccac tgcaatgaga cgacgctgcc catctacagc cgaagcaatg
     8221 agattaaacc cggcaccaga agtatgaccc gttttcaggc cgtcaacatt catggtttta
     8281 tcccacaata acccgttacg gttttgctgg gtgataccgt tccaggtgag acttttctca
     8341 ctgtacatat gataaaactc gggctcgccg tggatgatag cgcgagaaag cacagctaaa
     8401 tcataagccg agctatgctg acctggtgca tccagaccat gcactgtttc aaaatgcgta
     8461 tccttgagat gcagcttctc ggcatagttg ttcatcattt caacaaactg ccgttgccca
     8521 ccggcaatat agtcagccag tgcaacacaa gcgtcatttc cggaatccac aattaaacca
     8581 cggcttaaat cacgtaccga tacgcgatcg ccctctttca aaaacatcag tgaagaaccg
     8641 acaaacactg gattatcttt cgcccacgca tcgcgcccca cggtgacaat atcgtctggc
     8701 gtaatgcgat gactatcgat agcgcgatcc acgacatagc ccgtcatcag ctttgtcagg
     8761 ctggcgggat tgcgctgttg atgctcatta cccgcggtga ggatctgacc ggtggtgtaa
     8821 tccatcaata cccaggagcc ggcatgaatc tctggaggct gaggtgaaaa aggaatgttt
     8881 tccgccgcaa aaccagacga taagttaaaa atgaacaaag aagcagcaat aataagacgg
     8941 cgtttcaaca gcaaaccctc aggagtttca aatagctgtt ctttttacgg aaatacttat
     9001 gaactggctg gaataaagtg caagaaaatg tgactaccct ctcattttta tctgacatga
     9061 tctgttgcca ctcgctgtca aattgtcgcg ctaaagctga ttagcacggt gatatttgat
     9121 actctggcag acagcagaaa caacggattt aacctaatga tgaatgacgg taagcaacaa
     9181 tctacctttt tgtttcacga ttacgaaacc tttggcacgc accccgcgtt agatcgccct
     9241 gcacagttcg cagccattcg caccgatgac gaattcaatg tcatcggcga acccgaagtc
     9301 ttttactgca agcccgcgga tgactattta ccccagcctg gagcagtatt aattaccggt
     9361 attaccccgc aggaagctcg ggcgaaagga gaaaacgaag ccgcatttgc cgcccgtatt
     9421 cactcgcttt ttaccgtacc gaagacctgt attctgggct acaacaatgt gcgtttcgac
     9481 gacgaagtca cacgcaacgt tttttatcgt aatttctacg atccttacgc ctggagctgg
     9541 cagcatgata actcgcgctg ggatttactg gatgttatgc gtgcctgcta tgccctgcgc
     9601 ccggaaggaa taaactggcc tgaaaatgat gacggtctac cgagctttcg ccttgagcat
     9661 ttaaccaaag cgaatggtat tgaacatagc aacgcccacg atgcgatggc tgatgtgtac
     9721 gccactattg cgatggcgaa actggtaaaa acgcgtcaac cacgcctgtt tgattatctc
     9781 tttacccatc gtaataaaca caaactgatg gcgttgattg atgttccgca gatgaaaccc
     9841 ctggttcacg tttccggaat gtttggggca tggcgcggca ataccagctg ggtggcaccg
     9901 ctggcgtggc atcctgaaaa tcgcaatgcc gtaattatgg tggatttggc aggagatatt
     9961 tcgccattac tggaactgga tagcgacaca ttgcgcgagc gtttatatac cgcaaaaacc
    10021 gatcttggcg ataacgccgc cgttccggtt aaactggtac atatcaataa atgtccggtg
    10081 ctggcccagg cgaatacgct acgcccggaa gatgccgacc gactgggaat taatcgtcag
    10141 cattgcctcg ataacctgaa aattctgcgt gaaaatccgc aagtgcgtga aaaagtggtg
    10201 gcgatattcg cggaagccga accgtttacg ccttcagata acgtggatgc acagctttat
    10261 aatggctttt tcagtgacgc cgatcgtgca gcaatgaaaa ttgtgcttga aaccgagccg
    10321 cgtaatttac cggcactgga tatcactttt gttgataaac ggattgaaaa gctgttgttc
    10381 aattatcggg cacgcaactt cccggggacg ctggattatg ccgagcagca acgctggctg
    10441 gagcaccgcc gccaggtctt cacgccagag tttttacaag gctatgcaga agagattcag
    10501 atgctggcgc agcagtatgc cgacgataaa gaaaaagtgg cgctgttaaa agcgctttgg
    10561 cagtacgcgg aagagattgt ctaatgtgaa gcctccgccg ctggtacggc ggaggacaat
    10621 gttgagtcag gctttttgaa cggtgatgct ccacgctgca tcgccaatct gctgataatc
    10681 ggtgatggca tgcccttctt ctgccgccca ctgtgggatt gcttcagttg cctgggtgca
    10741 atcaaactca attaccaact catcgccgct ggccatttct gccagtgctg cttttgcttc
    10801 tatgagtgga aacggacaga cctgcgtcac cacatccagc tttttaatcg ccatctttac
    10861 accttactta atttgccgca gcagttgcca gtcgcgcctt acgctgcggt cgaacataca
    10921 caagccagga cgcagtccag actccgagaa tcataaatac aaggccaacc cagccctgcc
    10981 aggtcatcat tgcagtcata accagtccat taccgataga gcaaccacct gcaatactgg
    11041 caccgaagcc catcagtaca ccacctagcc cgctacgtac tgttgtttgt gcatcagctg
    11101 cgcgaacgcg gaactcacgg ctcgctttgg ctgcaataaa tgcccccacg aagatcccta
    11161 acaccaggaa aacgccccag ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa
    11221 tattggccgt tggggaagtg atgcctaacc caaacatacg tccggttgct tcactcaggg
    11281 gccaggctaa aagcgcaatc aaaccgatga gtacagctgt gacaaaggga tgccagcgtt
    11341 tttcaaacag aatatgagcg atcccggttc ggcgcggcgg taaggtcgcg actttgagtt
    11401 ttggcttctt caactctttc atcaccaccc agagcgttat caccagcaaa acggcaacca
    11461 acggccacac agacaaatta aaagtctcag caatagagtt atgttcagta gtgtagtgct
    11521 gcaaggtttg atttaaacca ctggcgtgtg gagaacgcat caccgcactc atcaccatat
    11581 aagtgaaaag cgcgatccaa ctgccgatca atccctcacc cgcgcgatac caggtcccgg
    11641 tggcacatcc acccgccaga acaattccca gcccgaagag atacccacct ataacagtac
    11701 caagccacgg gaacgccccg gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa
    11761 cccccacgct ttgtaccgaa attgcaatca gcaaggcgta aaacatgcga ttatttttcg
    11821 cgatatacat atcgcgaaaa ccaccagtca ggcaaaaacg cccacgctgc atgacaaatc
    11881 ccagcagagc accacaaatt agcccgctta atatcattga aaacataaat taattaacca
    11941 gatgaatgtt aatggggaaa ttattcatga ctggtggaat gcagaccaat aaccaaattc
    12001 tctaaattag aacaaatgg
//
LOCUS       DAZFKY010000145        11738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFKY010000145 DAZFKY010000000
VERSION     DAZFKY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..339
                     /gene="espR1"
                     /locus_tag="ACHKCL_003551"
     CDS             <1..339
                     /gene="espR1"
                     /locus_tag="ACHKCL_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO5764439.1"
                     /translation="QLMLFNLFSPALKLNTGLAILSPGAFEDHSDGLDVDNELFHYTI
                     NKAYTPYNIHTYKTEEVVNQRNIKIKNMTLDEINNTYCNNDYYNEAIREEPIDFLDRS
                     FSSSSWPFYH"
     gene            645..2033
                     /gene="narU"
                     /locus_tag="ACHKCL_003552"
     CDS             645..2033
                     /gene="narU"
                     /locus_tag="ACHKCL_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5764440.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2115..5855
                     /gene="narZ"
                     /locus_tag="ACHKCL_003553"
     CDS             2115..5855
                     /gene="narZ"
                     /locus_tag="ACHKCL_003553"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5764441.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5852..7396
                     /gene="narH"
                     /locus_tag="ACHKCL_003554"
     CDS             5852..7396
                     /gene="narH"
                     /locus_tag="ACHKCL_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5764442.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7396..8091
                     /gene="narW"
                     /locus_tag="ACHKCL_003555"
     CDS             7396..8091
                     /gene="narW"
                     /locus_tag="ACHKCL_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5764443.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8088..8768
                     /gene="narI"
                     /locus_tag="ACHKCL_003556"
     CDS             8088..8768
                     /gene="narI"
                     /locus_tag="ACHKCL_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5764444.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8847..9740
                     /gene="yddE"
                     /locus_tag="ACHKCL_003557"
     CDS             8847..9740
                     /gene="yddE"
                     /locus_tag="ACHKCL_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5764445.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9836..10680)
                     /gene="nhoA"
                     /locus_tag="ACHKCL_003558"
                     /pseudo
     CDS             complement(9836..10680)
                     /gene="nhoA"
                     /locus_tag="ACHKCL_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10979..11422
                     /locus_tag="ACHKCL_003559"
     CDS             10979..11422
                     /locus_tag="ACHKCL_003559"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5764446.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11426..11653)
                     /gene="pptA"
                     /locus_tag="ACHKCL_003560"
     CDS             complement(11426..11653)
                     /gene="pptA"
                     /locus_tag="ACHKCL_003560"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO5764447.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2806 a         3017 c         3058 g         2857 t
ORIGIN      
        1 cagctcatgt tatttaattt gttttcccca gcacttaagc ttaatactgg cctggcaatt
       61 ctttcgcctg gtgcatttga agatcactct gacggattag atgtggataa cgaattgttt
      121 cactatacta ttaataaagc atatacccca tataatatac atacttataa aacagaagaa
      181 gttgtaaacc agaggaatat aaaaattaaa aatatgacct tagatgaaat aaacaatact
      241 tattgtaata acgattatta caatgaggca ataagagagg aaccgataga ctttctggac
      301 agatcgtttt cctccagctc atggcctttt tatcactaat tctataagtg tctggtcttg
      361 ttaaaaatat agttaatata caaccctttg gattcatcat ctgaagggtt gaatcatgac
      421 atgcgtacat agcttaaccc cgtttaatgg ttagcttgtt agttacattt agtaacacat
      481 aactggctgc ttaatcccca ttctgccccc gcggttacgc tttcttatct attctttcaa
      541 tcagcacttc acccatttca tccttaattc taataactgg ctattgcgcc ttacctcctg
      601 ttttgtcttt ttttcattac caatgtgtac atgtgaggaa caatatggca ctgcaaaatg
      661 agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctggaaaa
      721 ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt ctacttcttg
      781 ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa atcggtttta
      841 atttcactac cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat
      901 tacgtgtccc ctactccttt atggtgccta tattcggtgg acgtcgatgg acggttttta
      961 gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata
     1021 ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg
     1081 cttcgagcat gggcaatatc agtttcttct ttccaaaagc caggcaaggt agcgctcttg
     1141 ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt gcaccgctgg
     1201 tcatttttgt acccgtattt gcctttctcg gcgtcaatgg agtaccgcag gccgacggtt
     1261 cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactagcg attgccacga
     1321 tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcgtcaatt gccgaccagc
     1381 tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt gccaccttcg
     1441 gttcgtttat cggtttttct gcgggtttcg ccatgctggc aaaaacccag ttcccggatg
     1501 tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cggtcggtgg
     1561 gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac tttattttta
     1621 tggcgatttt cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaacttca
     1681 tcgcctttta cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta
     1741 ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcggatg aagatgaaag
     1801 gcggtagtga tgagcaagct cagagagaag ccgtcaccga aacggcggcg gctctgggct
     1861 ttatctcagc catcggcgca gtgggcggct tttttattcc gcaggcattt ggcatgtcac
     1921 tcaatatgac cggctctcca gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt
     1981 gtgtgctact gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg
     2041 atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga
     2101 gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg
     2161 atacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct
     2221 atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag
     2281 gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga
     2341 ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc tgcccgcgtg
     2401 gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg ctcattcgta
     2461 aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg gtactggcgt
     2521 gggcatcgat tatgaacgat ccgcaaaaga gcctgagcta caaacaagtg cgtgggcgcg
     2581 gcgggtttat ccgctccaac tggccggaac taaaccagct gattgccgcc gctaacgtct
     2641 ggactatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt
     2701 cgatggtttc ttacgccgcc ggaacacgtt atctgtcact attaggtggc acctgtttaa
     2761 gcttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa
     2821 ccgacgtacc ggaatctgcc gactggtata actccagtta catcatcgcg tgggggtcta
     2881 acgtaccgca gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca
     2941 ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc
     3001 tggcaccaaa acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa
     3061 aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc cgctatagcg
     3121 acatgccgat gctggtaatg ctggaacctc gcgacgatgg tagctacgta cccgggcgca
     3181 tgatccgcgc gtctgacctg gtagatggac taggcgaaag caataatccg cagtggaaaa
     3241 ccgtggcggt taataccgca ggtgaattgg tagtgccgaa tggttcgatt ggtttccgct
     3301 ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtaaggaa accgaattgt
     3361 cgttaaccct gctcggtcaa catgacgccg ttgcaggcgt ggctttcccc tactttggcg
     3421 gcatcgaaaa tccgcatttt cgcagcgtca aacacaatcc tgtcctggtg cgtcaattac
     3481 ctgttaaaaa cctgacatta gccggtggca gcacctgtcc agtggtcagc gtttatgatt
     3541 tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt
     3601 acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc
     3661 agtatattga aaccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct
     3721 cgatgattat cctcggcgca ggtgttaacc actggtatca catggacatg aactaccggg
     3781 gaatgatcaa tatgctgatc ttctgcggtt gtgtcggaca aagcggtggc ggctgggcac
     3841 actatgtcgg tcaggaaaaa ctgcgcccgc aaaccggctg gttgccgctg gcctttgcgc
     3901 tcgactggaa ccgaccaccg cgccagatga acagcacctc gtttttctac aatcattcca
     3961 gccaatggcg ctatgaaaaa gtctctgcgc aggagttact ttcaccgctt gccgatgcca
     4021 gtaagtacag cggtcatctg attgatttta acgttcgcgc cgaacgcatg ggctggctac
     4081 cttctgcgcc gcagttgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg
     4141 gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta cgtatggcct
     4201 gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc
     4261 tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg gggaccgaaa
     4321 gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa gaagtcgagt
     4381 ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgcatgt
     4441 ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtat gaaaaagacg
     4501 atatgaacac ctcggatatg catccattta ttcatccgct ttctgcggcg gtcgatccgg
     4561 cctgggaatc acgcagcgac tgggaaatct acaaaggtat tgccaaagca ttttcgcaag
     4621 tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt acaaccactg ctgcatgact
     4681 ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc
     4741 tgagcccggg taaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacgt
     4801 atgaacgctt tacctcgctc gggccattga tggacaaact tggcaacggc ggtaaaggga
     4861 tttcgtggaa tacgcaggat gaaatcgatt tcctcggtaa actcaattac accaagcgtg
     4921 atggcccagc gcaggggcgt ccgctgattg acaccgccat tgacgcttca gaagtgattc
     4981 tggcactggc accagaaacc aacggtcatg ttgcagttaa agcgtggcag gcgctgggcg
     5041 agatcaccgg acgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct
     5101 ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tctggtctgg
     5161 aaagcgatca cgtctcctat aatgcgggat acaccaacgt tcatgagtta attccgtggc
     5221 gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg
     5281 aaagcctggt ggcatatcgc ccgcctatcg acacccgtag cgtcagtgag atgcgccaga
     5341 tcccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg
     5401 gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga
     5461 ttgtctggat cagcgaaacc gatgcccgtg aactaaccat tgtcgataac gactgggtgg
     5521 aagtgtttaa cgccaatggc gcgctgacgg cccgcgcggt ggtcagccaa cgtgtaccgc
     5581 cgggtatgac catgatgtat cacgctcagg aacgcattat gaatattcct ggttcggaag
     5641 taactggcat gcgcggcggt attcataact cggtcacccg catttgcccg aaaccaacgc
     5701 atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc accgtcggct
     5761 cgaaccgcga cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg
     5821 aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc cgttcgcaag tcggcatggt
     5881 gcttaatctc gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg
     5941 gaccggacgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat
     6001 tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg tccgcgatgt
     6061 gaatggcaag atacgcccgc gtctgggcag caagatgggt gtaataacca aaatcttcgc
     6121 caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca
     6181 tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cgctgattga
     6241 cggtaagcgg atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga
     6301 gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca
     6361 gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag
     6421 ctgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct
     6481 gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa
     6541 aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat
     6601 tgagtccggt caaccgaccg tgtgctcaga aacctgcgtg ggtcgcatcc gatatctggg
     6661 cgtgctgctt tacgacgctg accgcatcga ggaagcggcg agcaccgagc gcgaggttga
     6721 cctctatgaa cgccagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgagga
     6781 agccctgaaa caaggtattc cacaaaatgt gattgacgct gcccagcgtt cgccagtcta
     6841 caaaatggcg atggactgga aactggcact accgctgcac cctgaatacc gcaccctgcc
     6901 gatggtctgg tacgttcctc cgctgtcacc gattcagtcc tacgcagatg cgggcggttt
     6961 gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct
     7021 cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga aacggatgat
     7081 ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata ctcgtgccat
     7141 cgacgaagta ggcctgagca tcgcccaggt cgaagaaatg tatcgctatc tcgccattgc
     7201 caactatgaa gatcgttttg ttatcccgac cagccatcgg gaaatggcgg gcgatgcctt
     7261 cgcagaacgc aatggctgcg gttttacctt tggtgacggt tgtcacggct cggacagcaa
     7321 attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa
     7381 agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac
     7441 gagatgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga tgcgccgatg
     7501 cttacggatt tcacccgcaa cctgcttaac gcgccgctgc tggataaaca ggccgaatgg
     7561 tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca tgttcatgcc
     7621 gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggc
     7681 ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga gtatttaagc
     7741 gtactgcctg acgatcaggc gaaagaagga ttgctcaacg ttgcgccgat cctcgccctg
     7801 cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga tgctctgctg
     7861 caactggcgg gaagtacact ttcaagtgac agtgtcacaa aacaagtaaa cagcgaagag
     7921 cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt gaagtttatt
     7981 gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagt
     8041 caggatgtcg cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg attcagtatc
     8101 tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt ttcctcggca
     8161 gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt cagatgctcg
     8221 ataaacgcgg gatggtgata tggtcgaatc tgttccatat cggcattttg gggatcttct
     8281 tcgggcacct gttcggcatg ttaacgccac actggatgta cgcgtggttt ttgccagtgg
     8341 cagcgaaaca actaatggcg atggtgctcg gtggtatttg cggtgtgttg acgttgattg
     8401 gcggcgcagg actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc acttccacta
     8461 cgccggatat catcatcatg agcattttgc tgatccagtg tctgttgggg ctaagtacca
     8521 taccgttctc agcacagtat cctgatggta gcgaaatgat gaagctggtg ggttgggcgc
     8581 aaagcattgt cactttccgt ggtggatcgt cagagatgct taacggtgta gcgttcgtct
     8641 tccgcctgca tctggtgttg ggaatgacca tcttcttgct cttcccattc acccgactgg
     8701 tgcatgtgtg gagcgccccg tttgagtact ttactcgtcg gtatcagatt gtgcgctcgc
     8761 ggcgataatt tcaccctggc cggatagccc ttcgctctcc ggccacttgc aggctatgat
     8821 agttctccag tcacaggaga acgttaatga aaccgcaggt ttaccacgtc gatgctttta
     8881 cctctcaacc atttcgcggc aattctgccg gtgttgtttt ccccgccgat aatcttagcg
     8941 aagcgcaaat gcagcttatc gcccgcgaat taggtcattc ggaaaccgct tttctgctgc
     9001 acagcgacga cagcgatgtg cgcatccgct actttacgcc aacagttgaa gtgcctattt
     9061 gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt ttaggaaatt
     9121 gcacggtctg gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcaccacg
     9181 atggttatcg tatttcgctg gaacaaggta cgccaggctt tgagccaccg ctggaaggtg
     9241 aaacacgtgc ggcgattatc aacgcgcttc atctcactga ggacgatatt ctgccaggct
     9301 tgccaattca ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag
     9361 tggatatcga cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg
     9421 gctgcaatgg tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc gatggtcgca
     9481 tgttctcgcc tgcgattggt attgtggaag atccggtgac tggcaatgcc aatggcccga
     9541 tgggtgcatg gttggtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta
     9601 aaggccatca ggggcgcgca ttggggcgtg acggtatgat tgaagtgacg gtgacaattc
     9661 gtgataacca accggaaaaa gtcaccattt ctggcgcggc ggtgattttg tttcatgccg
     9721 aatgggcaat taaactctga aaccgttgcc tgatgcgctt cgcctgtcag gtctacaacc
     9781 gggctaatgt atgccggata aggcgtttac gccgcatccg gcaacctgac ataaattatt
     9841 ttcccgcctc cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacggtaa
     9901 agccatgttt cgcatcatcc acgcccagac caaactgttc ttgcatcaca gcatataacg
     9961 acgccacatc cggtagattt cgctgctcca ccgcgtgccc attttcataa tgggaaaatg
    10021 aaaattggtc agtgtcagct tgccgccgtc cggcaaatgg cggcacatca gcaaatgatg
    10081 gcgaaaatgc gactgcggcc agtgcgccga ccagaaatta cccatcacat aatcgctttg
    10141 ttgctgctcg cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg
    10201 caacacccaa tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg tggtctgcac
    10261 aagatcggga actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc
    10321 aatccatttt tcctcttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc
    10381 tggcggattt gataacacta cccgccctaa caagctgcga acgttaaacc ccagctcgcg
    10441 taacacccgc tcaaaaacgc cattctgctc aaaacagtaa ccgccacgac gggctatcac
    10501 cagtttctct tccagcgatt gatcatcaag ctgcatttcc ctcggcagca aaacgtcgag
    10561 gttttcaaac ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt
    10621 gaccgcagca gctcccgacc agttaatacg ggcaaaatag tgattcagaa tgggcgtcat
    10681 caggcgtttc ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta
    10741 ttttgtgcga tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta
    10801 cttattgcgt tacggactac gcttaaaaaa accttcttcg cgaggcgata ccatgagccg
    10861 attcatcccc atagaattac accatgccag ccggctgtta aatcatggcc caactgtcat
    10921 gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt atggctgcag cctgatcaat
    10981 gccggtggag tttgaacctc cacgcgtgac gattgtggta gataaatcga catggaccag
    11041 ggagttgatt gaacgtaacg gtaaatttgg catcgttatc ccgggcgttg cagcaactaa
    11101 ctggacgtgg gcagtgggaa gtgtgtcggg tcgtgaagaa gataaattta attgctatgg
    11161 cattccggtt gtgagaggcc cggtatttgg tttgcctctg gtcgaggaaa aatgtctggc
    11221 gtggatggag tgtcgattgc tacctgcgac ttctgcgcaa gaagaatacg acacgctgtt
    11281 tggcgaagta gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt
    11341 tgatgatgat aagctcaata cgttgcatca tttaggtgct gggacgtttg ttaccagcgg
    11401 caagcgtgtt acggcgggtt aagcattaca tgctgtaacc gggtttcttt atcaaagcct
    11461 ccatttgggg cgcgatttcg gcatcccaga tagcttgcca agattctggt tgaatctgtt
    11521 gtagagcaat gcttatcgaa ctgtctttac tgttcagatg gcgaataata acgtcggtaa
    11581 tatctgcagc aagtgctgct ttttgttgtt cgtccagttc acgcggaaaa catttaatgt
    11641 ctatgtgcgg catgattacg ctccttttga aagatgtcag taaaattaac acacattgtg
    11701 aatgtcgtgt gtccggtaaa gcgtttttac gacaataa
//
LOCUS       DAZFKY010000146        11725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFKY010000146 DAZFKY010000000
VERSION     DAZFKY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            206..4189
                     /gene="ehaA"
                     /locus_tag="ACHKCL_003561"
     CDS             206..4189
                     /gene="ehaA"
                     /locus_tag="ACHKCL_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO5764448.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4331..5419
                     /locus_tag="ACHKCL_003562"
     CDS             4331..5419
                     /locus_tag="ACHKCL_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5764449.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5461..6392)
                     /locus_tag="ACHKCL_003563"
                     /pseudo
     CDS             complement(5461..6392)
                     /locus_tag="ACHKCL_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6484..6991)
                     /locus_tag="ACHKCL_003564"
                     /pseudo
     CDS             complement(6484..6991)
                     /locus_tag="ACHKCL_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7249..7854
                     /locus_tag="ACHKCL_003565"
     CDS             7249..7854
                     /locus_tag="ACHKCL_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5764450.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7894..8757
                     /locus_tag="ACHKCL_003566"
     CDS             7894..8757
                     /locus_tag="ACHKCL_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5764451.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8747..10294
                     /gene="fdrA"
                     /locus_tag="ACHKCL_003567"
     CDS             8747..10294
                     /gene="fdrA"
                     /locus_tag="ACHKCL_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5764452.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10294..11712
                     /locus_tag="ACHKCL_003568"
     CDS             10294..11712
                     /locus_tag="ACHKCL_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5764453.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3098 a         2726 c         3168 g         2733 t
ORIGIN      
        1 aagttaagag tgttaactta aaataagttt ttagtagtaa cttgctattt tattaccctc
       61 tgaactacat actcagatat gtggttgcca atgctatgca aaatttttga atttttatat
      121 gcgatgaaca gtaaccggaa tggcatttaa tgcgttgctg tttatgcaat cttggtttta
      181 tttggatgta ttgctggaga ttgtgatgaa taaaatatat cggctaaagt ggaacaggtc
      241 ccgtaactgt tggagcgtct gctcggagct ggggagcaga gtaaaaggaa aaaagtcccg
      301 ggctgtttta attagcgcga taagtttata ttcatctctg gtattcgccg atgatgtcat
      361 cgtaaaccag gataaaacta ttgattttgg caaagagaac cagagcatcg attaccgtat
      421 tacggtgaca gacaatgcca atctggtaat caatgcgaca gatacttccc gtccgcgtct
      481 gactctcgct tctggtggtg ggttggatat taccggagga aaggtaacaa tcaatggccc
      541 acttaacttt ttgctgaaag gtacggggtt cctgaatgtc tccaatgctg gcagcgagtt
      601 atatgctgat gatttgtatg aatcaaactc aggcatgaga cacgatcgcg gctattttaa
      661 tgtctccaac ggcggcaaaa tccatgttaa gggcaccagc cgtctgacct atttgcaggg
      721 aaatgtcagt ggtgaaggta gccaggtaaa ttccgaaacc ttctttatgg gcgtttacgg
      781 cagttacggt ggtaatcagt acctgtcagt taataacggc ggtgaagtta atgccaggaa
      841 gcaaattagc ctgggctatt atgatcaagt ctccgataca acacttgctg tttcggaagg
      901 tggtaaaatt tctgcgccta ctattagttt aagcaccaac tctgagttag cgttaggggc
      961 acaggaagga agcgcagcga aggcagcagg gattattgat gccgaaaaaa ttgagtttgt
     1021 gtgggcaaag acatccgaga agaaaatcac cttaaaccac acggataaag acgcgactat
     1081 ttccgcggat attgtcagtg gcagcgaggg cctgggctat atcaatgcgc tcaatggcac
     1141 gacttactta accggtgata actctgcctt tagtggtaaa gtcaaaattg agcaaaatgg
     1201 cgctttaggg atcacccaaa atataggtac agcagagatc aacaaccgcg ggaaattaca
     1261 cctgaaggct gacgatagca tgacctttgc caataagatc tctggcaacg gtacaataag
     1321 tatcgacagt gggacggtgg agttgaccgg caataactat gcattcagcg gatatattga
     1381 tgttgcttct ggtgctgtcg ctgttatttc tgaagacaag aatatcggtc gtgcagagct
     1441 ggatgtcgat ggcaaattgc aaattaatgc caacaaagat tgggtatttg ataacgatct
     1501 tgaaggtaga ggcattgttg aaataaacat ggggaatcac gaattctcct tcgatgagtt
     1561 tgcttataca gactggttcc agggttcact ggcgttccag aacacgacat ttaatctgga
     1621 aaagaatgct gagtttctgc agaaaggcgg gatcactgcg ggtcagggaa gcctggtaac
     1681 agtgggtaag ggcgctcact ccattagcac tttgggattc tccggcggaa ccgttgattt
     1741 tggtgccctg acagcaggtg cacagatgac agaagggacg gtcaacgtta gtaaaacgct
     1801 ggatttgcgc ggcgagggtg tgattcaggt ttctgacagt gacgttgtcc gctcagtatc
     1861 tcgtgatatt gactctgcgt tatcgctcac tgaagtcgat gatggtaaca gcaccattaa
     1921 gttggttgat gcgcaaggtg cggaagttct gggcgatgcg ggcaatctgc aattgcagga
     1981 taaaaatggg caaatcctct ccagcagcgc ccaacgtgat attcagcaga atgggcaaaa
     2041 agcggccgtc ggcacttacg actatcgtct gacgagtggg gtaaacaatg acggtctgta
     2101 tattggttac ggcctgaccc agcttgattt acacgctacc gacagcgatg ctctggtgct
     2161 gagctctaac ggtaaaagcg agaatgccgc cgatctcagc gcaaagatta ccggcagtgg
     2221 tgacctggca ttcagcagcc agaagggtca gaccgtatcg ctttctaaca aagacaacga
     2281 ctataccggt gttaccgatc tgcgcagtgg gacgcttttg ttgaataacg ataacgtgtt
     2341 gggtaatacc catgaactgc gtctggcggc agagactgaa ctggacatga atggtcacag
     2401 ccagaccgtg ggcacgctca atggcagcgc cgattcactg ctgagcttaa atggcggcag
     2461 tctgacggtt accaacgggg gcacttcaac cggttcgtta acggggagcg gagagctgaa
     2521 tattcagggc ggcacgctgg acatcgcggg cgataacagc aacctgacgg cgaatgtgaa
     2581 cattgctaat tcggctaatg tcctggtaag tcatgcgcag ggattgggta gcgcaaacgt
     2641 tgagaacaac ggtaccctgg cgttgaataa tagcgctgaa aaaagagcgg ctgcgtctgt
     2701 gaattacgcc ctgggcggca atctgaccaa caacggtacg ctgatgaccg gaatgtcagg
     2761 acagcaagct ggcaatgtgt tagtggtgaa ggggaactac cacggtaata acggtcaact
     2821 agtaatgaat acggtactga atggcgatga ctcagtaacc gataaattgg ttgtcgaggg
     2881 cgatactagc ggcacgactg ccgttacggt gaataacgct ggcggtacag gtgcgaaaac
     2941 ccttaacggt atcgaactta tccatgtaga cggtaagtct gagggcgaat ttgttcaggc
     3001 tgggcgtatc gttgcggggg cgtatgacta cactctcgcg cgtggacaag gggcaaatag
     3061 tggtaactgg tatctgacca gcggcagtga ttctcctgaa ctgcagccgg agccagaccc
     3121 gatgccgaat ccagagccaa acccgaatcc agagccgaac cctaacccga cacctacgcc
     3181 gggtccggat ctgaatgtgg ataatgacct gcgaccggag gcgggtagct acattgcgaa
     3241 ccttgcagca gcgaatacca tgttcaccac gcgtctgcat gagcgtctgg gtaatacgta
     3301 ctataccgac atggtgacgg gtgagcagaa acaaaccact atgtggatgc gccatgaagg
     3361 tggtcataat aaatggcgtg atggcagcgg ccagctgaaa acccaaagca atcgctatgt
     3421 tctgcaactg ggaggcgatg tcgcgcagtg gagccaaaac ggcagcgacc gctggcatgt
     3481 tggggtcatg gcgggatatg gcaacagcga cagcaaaacc atttcctcgc gaaccggtta
     3541 tcgtgcaaaa gcgagtgtga acggatatag cacaggcctc tatgccacct ggtatgccga
     3601 tgacgagtcg cgtaatggcg cgtatctcga cagttgggcg cagtacagct ggtttgataa
     3661 cacagtgaaa ggggatgact tacaaagtga atcctataaa tcaaaaggat ttaccgcttc
     3721 actggaagct ggatacaaac acaaattagc tgaatttaat ggcagccagg gaacgcgtaa
     3781 tgaatggtat gttcagccgc aagcacaggt tacctggatg ggagtcaaag ccgataagca
     3841 ccgcgaaagc aacggaaccc tcgttcatag caacggtgat ggcaatgttc aaacccgact
     3901 tggcgtaaaa acctggctga agagccacca taaaatggat gacggtaaat cccgcgagtt
     3961 ccagccgttt gtagaagtga actggctaca taacagtaag gatttcagca ccagtatgga
     4021 tggcgtgtct gtcactcagg atggagcccg aaatattgct gagataaaaa ccggggtgga
     4081 aggacagcta aatgccaacc tgaatgtctg ggggaatgtg ggcgttcagg ttgccgatag
     4141 gggatataat gacacctctg caatggttgg cattaagtgg caattctgat ctgaataatg
     4201 agtaaaagga gagagagccg aacgggagat aattcggctc tctgtgtcat tttcttgcag
     4261 ccagctttat ccgtcagagt gaagactcgc agatttagct ggcgcacaag atttaatcaa
     4321 ggtgtgttca atggcttcgc atgatttgag tgtttttttg gaagagttcg gggcaacggt
     4381 gaatttgacg ttgcctggta tcgtgtccga aaaagaacgg cttttactca agctgctaat
     4441 ggagggaatg tcggttacag aaatctcaca gtacagaaat cgcagtgcaa agacaatttc
     4501 acatcaaaag aagcagctgt atgaaaaact aggcatccag agtgatatta ccttctggcg
     4561 ggatatcttt tttcaatatc atccgcaagt cattagtggc acgggaaata aaaataattt
     4621 ttatatccct gataaccgtt gtcaccatat cgtcacgcct gaagccatca gtctggcgtt
     4681 ggaaaaccat gaatttaaac cgtggatcca accggttttc tgcgcgcaga ctggggtact
     4741 gacgggctgt gaagtgcttg tccgctggga acatccacaa acgggaatca tcccgccgga
     4801 tcagtttatt cctctggcgg agtcatccgg tcttattgtc ataatgaccc gccaactgat
     4861 gaaacagact gcggatattc tgatgccggt aaaacatttg ctgccggata atttccatat
     4921 tggtatcaac gtctcggcgg gttgtttttt ggccgcggga tttgaaaaag aatgcctgaa
     4981 cctggtcaag aaattaggaa acgataaaat caaactggtt ctcgagctaa cggaacgtaa
     5041 ccctattccg gtaacgccag aagccagagc gatatttgac agccttcatc agcataacat
     5101 tacctttgcg ctggatgact ttggtacggg ttatgcgacc tatcgttatc tgcaggcgtt
     5161 tccggtcgat tttattaaga tcgataagtc atttgtgcaa atggcgggtg tcgacgaaat
     5221 atccggtcat attgtggaca atattgtcga actggcgcgt aagcctggtc tgagtatcgt
     5281 ggcggaaggg gtagaaaccc aggagcaggc ggatttaatg atcggtaaag gcgttcactt
     5341 tttgcagggc tatttgtact ctccgccagt accgggtaat aaatttatct ctgaatgggt
     5401 aatgaaagca ggtggttgat gtaaaccgct attcacagcg catcgggagg ttggcagcga
     5461 ttaattctcc cgatgcagta aatcctgata gatccccgtt aatatgccat ttggcgcaaa
     5521 ttgtttttta atccactgcg tcacctgacc cgtagcagaa tgctgagtcg ccagtaacat
     5581 gcgtgaatcc tggcgcgggt tatggatttg tcgggtaacc agcagggatt gcgccatcgc
     5641 ctcacggacc atgtaatctg gcaaaaaaac aatcccttca cctaaaatct ggcactgaca
     5701 tttggtgttg aaatctggca ccagaattga ctcctgcccg tgcagcaacc agcccacttt
     5761 tttattaatc gtatgcgcgg tgtcctccac cataatatta gggtagagac gcagttggct
     5821 ttcggcgatg gttccggcac aaaggctaac gggtgatccg gggcgatagc aaaagcccag
     5881 cgaatcgcgc caatttcagt gtaatcaata ccgccgccgt ccagcagcgt gtcgggtgcg
     5941 ccgatagcga tatttgcctg attattaata atcgcatccc agacgccgtt atacacttcg
     6001 gtggtgacgg taatctggca ggtaggaaac tgctttttca gcacctgtaa taagcgtgcg
     6061 gtgtgtttgg gcgtataaag caactgatta atacaaatgc gcacacgcgc ttctatgccc
     6121 tgcgaaatag tatcgatgct gcgtttgatg gcgtgaaaat cattcagcag gtcggtagct
     6181 ttgcggaaaa aataacgccc ggactccgtt aactcaatgc tgcgggtact gcgagtgaac
     6241 agcaccacat ccagccctgt ttccattcgc ttgatggtgt agctaatggc ggaagtggtt
     6301 aaacccaact cttctgcggc tttactgaaa ctgccaaaac gcgcagcggt agtaaatgcc
     6361 aacagattct cttcggtaaa aattgagttc attaaaccat tcccgccatt atctaatatg
     6421 aatttctcca agcataactg tagaggtatc aattagagat gggtaatcat ttttgaataa
     6481 cttttaatac ccgtcgcgtt tagtcgtcgc ttcgcttaat ttttgtccgc catattctga
     6541 aattatcccc acgcagttac ccgccagaaa acccagtaac gtcaccgtcc agttcatccc
     6601 gctggcaaaa aacagcgtca tgccgagaaa gccaccggga ataaatgaca gtaaccagaa
     6661 acgcccttgc cagaccacca ccgccgagaa gggtacggta gtaattacgc tcgcccagaa
     6721 cgcggacagt ccacttacag aggccagcca accggcagcc agcgcggcga aaaacgccca
     6781 caccatcccg gaataattaa ccgacagact tctgataaac cccgatttac cgccgcctgc
     6841 ggcataaaaa ctgcaaaagg cgacgaagcc aatggttcct aataattcct aataattccc
     6901 atccctgaga gagcccgaca tgtgaagaca ctaactgcca aagccctgca caaataccga
     6961 cggtgacacc cgttgccgtg agtccgttca tcttgttccc ctctggttag acatggacga
     7021 aagctactct tacatgtgca gactatagag aaataaaacc tgattgagtg aaagggtatc
     7081 gggtcaaaga aacaaatatt gcactaccgc acactgcgaa aagattgttg aacatcattc
     7141 aacaaaaagg cgaaatacgg cgaattaaat ttaagcgggt tcttttatca gattatcgtt
     7201 gctattctca attcatcgaa aacagccaca aaaaccaggg gaatgtgaat gagtataaaa
     7261 aatctacctg ccgactattt attggctgca caacagggtg atatcgataa agtaaaaacc
     7321 tgccttgcgc tgggtgtcga tattaatacc tgcgatcgtc aggggaaaac ggcaattacg
     7381 ctggcaagtt tatatcagca atatgcttgc gttcaggcat taattgatgc cggagcggat
     7441 attaataagc aagatcatac ctgtttaaat ccttttttaa ttagctgtct gaacggtgat
     7501 ttaacgctgc tacgaattat tttaccggct aaaccagacc ttaattgcgt cacccgtttt
     7561 ggcggtgtcg gcctgacgcc tgcctgtgaa aaaggccatt taagtattgt aaaagagctt
     7621 ttggcgcata cggaaattaa cgttaaccag accaaccatg ttggctggac gccactactg
     7681 gaagcgattg tgcttaacga tggcgggatt aaacagcagg cgattgtgca gttattgctg
     7741 gaacacggtg ccagcccgca tctgaccgat aaatatggca aaacgccact ggaactggcg
     7801 cgggaacggg gctttgaaga gattgcgcag ttactgattg ccgcaggtgc ataaaccggg
     7861 aggcttgcta tcaacacacc agaaaaacgg tgtgtgtggg cgctaactgc ggatgcggat
     7921 tttctggcgc agcgagggca aggacaggtt gaacaggtct ttgccagagc ggtaaatatc
     7981 gcactcccgg ctcgccagca gttgctgacg ctgctttgtg aagagtacga caatgcgcca
     8041 aacagttgtc ggttggcact cactcacttt gatgatctgt tccggcatgg tgataaggtt
     8101 cagtttgacg atcaaggtat tacggttggt caacatcttc atatagagat gagtcgttgt
     8161 cggcgttggc tgtccccaac cttgcaaatg accgctgtga attttcgcct tatcgcctgg
     8221 caacagtggc acgacatcat tcatcagcac ctgggggaaa atgaaaccct gtttaattat
     8281 cgcggcgata atccgtttta tcaggcgtta aataaagaat tacatattaa acgacgggca
     8341 gttatacagg cagtaatcga taaacaaaat atcgcctcag cggtcgccag tatgatgggc
     8401 ttagggattg gccttacgcc atcagccgat gattatttaa caggtctggc acttatttta
     8461 tttatttccg ggcatccggc gggaaaatac aaagaggaat tttatctcgg cttgcaacgc
     8521 ggcagaaata ataccacgtt attaagtgcc ataacgctgg aagccgcatt acaacaacgc
     8581 tgccgggaaa atattcatcg ttttattcac aacattattt atgacatccc tgggaacgca
     8641 actcaggcaa tagaaaaaat taaacatatt ggctccagtt ccggctgcga catgctgtat
     8701 ggcatggccg atggttgtgc gctgagccaa acctacggag ggaattatgt cagttaaaat
     8761 agtcattaaa ccgaatacct attttgattc tgtctcgctg atgtctatct ccacgcgtgc
     8821 aaataaactc gacggcgtcg agcaggcatt tgtggcgatg gcgaccgaaa tgaacaaagg
     8881 tgtgctgaaa aatttaggac tgctgacgcc ggagctggag caggcgaaaa acggcgacct
     8941 gatgattgtc atcaatggta aatcgggggc ggacaacgag cagttgctgg tggagattga
     9001 agaactgttc aacaccaaag cgcaaagcgg ctcgcacgag gcgcgttacg ccactattgc
     9061 cagcgccaaa aagcatatcc cggaaagtaa cctggcggtg atttcggtca acggtctgtt
     9121 tgccgctcgc gaagcgcgtc aggcgctgca aaacgatctc aacgtgatgc tgttttccga
     9181 taacgtctca gttgaagatg aactggcgct caagcaactg gcccacgaaa aagggctgct
     9241 gatgatgggg ccagactgcg gcacggcgat tatcaacggt gcggcgctct gctttggtaa
     9301 cgccgtgcgt cgcggcaaca tcggtattgt tggcgcatcc ggcaccggca gtcaggagtt
     9361 gagcgtccgc attcatgaat ttggcggcgg cgtttcgcaa ctgattggca ccggcgggcg
     9421 cgacctgagc gagaaaatcg gcggcctgat gatgctcgac gccatcggga tgctggaaaa
     9481 cgatccgcaa actgaaatca ttgcgcttat ctccaaaccg cctgcgcctg cggtggcccg
     9541 caaagtgctg gaacgtgcgc gcgcctgccg caagccggtg gtcgtttgct tcctcggtcg
     9601 tggcgaaacg ccagtggatg agcaggggct acagtttgcc cgcggcacca aagaggcagc
     9661 gctaaaagcg gtgatgctct ccggcgtgaa acaagaacat ctcgacctgc ataccctgaa
     9721 ccagccgttg attactgacg tgcgcgctcg cctgaagccg cagcagaaat acattcgtgg
     9781 cctgttctgc ggtggcacgc tgtgcgatga aacgatgttc gcggtgatgg aaaaacacag
     9841 cgatgtctac agcaacatcc agcccgatcc ggaattccgc ctgaaggata tcaaccgcag
     9901 catcaaacac accttcctcg actttggcga tgacgacttc accaacggca agccgcaccc
     9961 gatgattgac cctaccaacc gcatcagccg cttgatcgaa gaggcgcgcg atccagaagt
    10021 ggcggtgatc gtgatggatt ttgtgctcgg atttggatcg catgaagatc cggtcggttc
    10081 caccatcgag gcgatcaaag aagcgaaagc gatcgccgct gccgaaggac gcgagttgat
    10141 cattctcgcc tatgtgctgg gtaccgatct tgatacgcca tcgttagaac aacaaagcca
    10201 gatgctgctt gatgccggcg tgattctggc gagcagcagc accaataccg gattgctggc
    10261 gcgtgaattt atctgcaaag gggaggaagc ctgatgagcc agtcactgtt tagccaacca
    10321 ttgaacgtta ttaacgtcgg catcgccatg tttagcgatg acctgaaaaa gcagcatgta
    10381 gaagtgactc aattcgactg gacgccgccg gggcagggca atatgcaggt ggtgcaggcg
    10441 ctggataaca ttgccgattc gccgctggca gacaaaatcg ccgccgctaa ccagcaggcg
    10501 ctggagcgca ttatccagtc gcatccggtg ctgattggtt ttgatcaggc gattaacgtg
    10561 gtgccgggca tgacgccgaa aaccattctt cacgccgggc cgccgatcac ctgggaaaaa
    10621 atgtgcggcg cgatgaaagg cgcggtcacc ggagcgctgg tgttcgaagg actggcgaaa
    10681 gatctcgacg aggcggctga actggcggct tccggggaga tcaccttctc gccgtgtcac
    10741 gagcacgact gcgtgggatc gatggcgggt gtgacctcgg cctcgatgtt tatgcacatc
    10801 gtaaaaaaca aaacctacgg caacatcgct tataccaaca tgagcgagca gatggcgaag
    10861 attttgcgta tgggcgctaa cgaccagagc gtgattgacc gcctgaactg gatgcgtgat
    10921 gtgcagggac caatactgcg cgacgcgatg aaaattatcg gcgaaatcga tctgcgctta
    10981 atgctggcgc aggcgctgca tatgggcgat gagtgccata accgcaataa cgccgggacg
    11041 acgctgctga ttcaggcgct gacgccgggg attatccagg cgggctattc cgtcgcgcaa
    11101 cagcgcgaag tgtttgagtt tgttgccagt agcgattact tctccggccc gacgtggatg
    11161 gcgatgtgta aagcggcgat ggatgcggcg cacggcatcg aatacagcac cgtcgttacc
    11221 accatggcgc gtaacggcgt cgagttcggc ctgcgggtga gcggcctgcc ggggcagtgg
    11281 tttaccggcc cagcgcagca ggtcatcggc ccgatgtttg ctggttataa gccggaagat
    11341 tcggggctgg atatcggcga cagcgccatc acagaaacct acggtattgg cgggtttgcc
    11401 atggcgaccg cgcctgcaat cgtcgcgctg gtggggggca cggtggaaga ggctatcgat
    11461 ttctcccgtc agatgcgcga aatcaccctt ggcgaaaacc ccaacgtcac cattccgctg
    11521 ctcggtttta tgggcgtgcc gtcggcagtc gacatcaccc gcgtgggtag cagcggcatt
    11581 ctgccggtga tcaacaccgc catcgcccat aaagatgcgg gcgtcggcat gattggcgcg
    11641 ggcattgtgc atccaccatt cgcctgcttc gagaaagcca ttcttggctg gtgcgaacgt
    11701 tacggcgttt gacctacctg ttcat
//
LOCUS       DAZFKY010000147        11691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFKY010000147 DAZFKY010000000
VERSION     DAZFKY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..2476
                     /gene="ppsA"
                     /locus_tag="ACHKCL_003569"
     CDS             98..2476
                     /gene="ppsA"
                     /locus_tag="ACHKCL_003569"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5764454.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHKCL_003570"
     CDS             complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHKCL_003570"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5764455.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4236..4529)
                     /locus_tag="ACHKCL_003571"
     CDS             complement(4236..4529)
                     /locus_tag="ACHKCL_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5764456.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4526..5815)
                     /locus_tag="ACHKCL_003572"
     CDS             complement(4526..5815)
                     /locus_tag="ACHKCL_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5764457.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5871..6809)
                     /locus_tag="ACHKCL_003573"
     CDS             complement(5871..6809)
                     /locus_tag="ACHKCL_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5764458.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6829..7593)
                     /locus_tag="ACHKCL_003574"
     CDS             complement(6829..7593)
                     /locus_tag="ACHKCL_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5764459.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7909..8820
                     /locus_tag="ACHKCL_003575"
     CDS             7909..8820
                     /locus_tag="ACHKCL_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5764460.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(8863..10014)
                     /locus_tag="ACHKCL_003576"
     CDS             complement(8863..10014)
                     /locus_tag="ACHKCL_003576"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5764461.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10028..11623)
                     /locus_tag="ACHKCL_003577"
     CDS             complement(10028..11623)
                     /locus_tag="ACHKCL_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5764462.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         2829 a         2986 c         2929 g         2947 t
ORIGIN      
        1 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
       61 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
      121 gctggtgctt tggtataacc aactcggcat gaaggatgta gacagggttg ggggcaaaaa
      181 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
      241 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
      301 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
      361 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
      421 ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcttttg cggtgcgctc
      481 ctccgccacc gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct
      541 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
      601 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gtgtggcgct
      661 ctccgccggt gttcaacgga tggtgcgctc tgacctcgca tcttctggcg tgatgttctc
      721 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
      781 tgagatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcata aaccgacact
      841 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
      901 ttacgcgccg acccaggagc atggcaagca ggttaaaatc gaagacgtac cgcaggaaca
      961 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     1021 aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg gccacactgg
     1081 taaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     1141 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     1201 catcggtgcg ggtccggtga aagtcatcca tgatatcagc gaaatgaacc gcatcgaacc
     1261 tggtgacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     1321 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     1381 actgggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggtga
     1441 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     1501 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
     1561 gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgaacg aaggcgtggg
     1621 ccttgcacgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     1681 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     1741 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     1801 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     1861 caacctggtc ggtggtgagc gttatgagcc agatgaagag aacccgatgc tcggcttccg
     1921 tggcgcggga cgctatgttt ccgacagctt ccgcgactgt ttcgcgctgg agtgtgaagc
     1981 agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     2041 gcgtaccgta gatcaggcga aagcggtggt tgaggaactg gcgcgtcagg ggctgaagcg
     2101 tggtgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
     2161 cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgaca tgacgcagct
     2221 ggcgctcggt ctggatcgtg actccggcgt ggtgtctgaa ctgttcgatg agcgcaacga
     2281 tgcggtgaaa gcactgctgt cgatggcgat tcgtgccgcg aagaaacagg gcaaatatgt
     2341 cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt tgatggaaga
     2401 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc
     2461 tgaactgaag aaataaaata aatccccggc ggcgttttgt cgccgggttt atgtgatccc
     2521 cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaaccgcc gcataatatc
     2581 tttccgtaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt tttcgattac
     2641 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
     2701 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     2761 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     2821 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     2881 tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat cgccgctgta
     2941 ataccagcct tcttcatcca gggcacgggc ggttaattca ggttcatcaa aatatcccat
     3001 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aggtcttgcg
     3061 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgcataaa
     3121 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     3181 aacacttaat aatttaatgc cgcgctgctg gcattcacgc gccacttttt tggggattgt
     3241 ggtaccgccg caaagaaaga agcgcagcgc tgaaaggtct gcgggttgtt tctctagtaa
     3301 attcaaaaga tcatagacaa acggcgttgc gccgagcata caggagcaac gctgctgctc
     3361 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcgccaat
     3421 taagaatggt gccgttacgc catgcagaaa gcccgttgcg tgaccaagtg gcgcaggcat
     3481 cataaagaca tcctgccagg ttagattcag ccgcgcgcaa taagcccgct cactggcgag
     3541 aatattgtta tgcgtaagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
     3601 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc
     3661 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
     3721 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg gacgcgtttg
     3781 tttaaacaac gtcggtgtaa agaacatttt tgcctgacac ttattaagca cccataccag
     3841 ttctgcttcc cgccaggaag gcaacagcgg tacggaaacc gcaccgattt tcaggcaggc
     3901 aagatagata acggtaaatt cacaccagcc tggcagttga aatgctatgc gatcgcctga
     3961 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     4021 ataggtgtac gatgctccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
     4081 ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct gacgatacgc
     4141 cgcacgacgt tgttcgttaa acgttaatgt cactttcatt ttggactctc gaaacagaac
     4201 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
     4261 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
     4321 gtgccgcact ccagacaacc ggcggaatca aaatgaatgt ttcctgcgtc atcctgctta
     4381 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
     4441 gccaaaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
     4501 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     4561 gcaagttgat cagcccaatt ttcttcgcgt gtcccatgat cattttgcgt accggctggt
     4621 taggcttacc gtcaatggtg aacatctcgt tcatgatgtc ggctaccatt cgtgggtatt
     4681 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcgaaaatgc tgcatatcac
     4741 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgctg gcggagaaat
     4801 ccgcgcgttc tttggcggcg atcactgttg tggcggcagc ctgagccgat gcaatggcta
     4861 aatccatgcc gcggaccgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     4921 tcatcacgcc atcgttaacc agttgcggta ccatcgccag accgccttct ggcaccatat
     4981 gcgcggaata ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg gggtgttgtt
     5041 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca gccaatccac
     5101 aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg cccatcaggc
     5161 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
     5221 gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca gcgtaatgat
     5281 gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta acgccatcgg
     5341 ctaaaatcac cacattggct tcgagaatat catccccggc ctggactcca gtgaccttgt
     5401 ttccttcacg aaccaacgca tcgacgcgaa ctcccgggat aaactgtgcg cccgcctgct
     5461 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
     5521 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctctct tcggttaaga
     5581 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatgctgca aagcctggaa
     5641 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
     5701 tgtcgccgcg ttctatcacc aggatatcca gcccggctcg cgccatgacc agtgcggcaa
     5761 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt
     5821 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
     5881 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
     5941 tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tgcgccgttg
     6001 ccgccaacca tatgctggat ctgcccggag atccctagcg tcaggtagag atcggatttc
     6061 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
     6121 attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg gaccattttc
     6181 aggtcatcct gcgctgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
     6241 tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggagca
     6301 acgtaaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
     6361 acaccgggtg caagggtaat gatcgccagc gagctgttga tcttttcctg agcgaacgct
     6421 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
     6481 gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag cgctttacca
     6541 cgtttcgtcg cggccagtag caacatagcg gggtgtttat ctttcagcag agcagcaatg
     6601 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
     6661 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
     6721 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
     6781 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
     6841 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgagaatgat
     6901 gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc agttcagcaa gtggtgcgct
     6961 ctggctccag ccaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
     7021 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
     7081 tggaacagag agttcaataa cttcaacgtc atcttcaagc gtgcgttcaa tcaccagtgt
     7141 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
     7201 gaccagcaag ccaacctgct gggcataaag gtcgccggaa cctttaccaa agatcagtaa
     7261 atcgaagccg atcttttcaa ctgctgccgc cagcgctttt gcggtatcga gaggcagtgc
     7321 atgctcaagt tgtgcatcct gcaccatata caggctgtgc ggcccgcggg atagcacgtc
     7381 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
     7441 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
     7501 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
     7561 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
     7621 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
     7681 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
     7741 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
     7801 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
     7861 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
     7921 tgttttgata atgccagtga aacgctgttt gtcgctggta aaacgccacg gctttcacgt
     7981 tttgcattta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
     8041 gagctgattt acgtcaagaa aggggtggca aggtttacca tcgattcttc gttatatgtc
     8101 gcgcatgcag atgatatagt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac
     8161 gttaacgatc cggcaacgac gtgtacttgt gcgctgtacg gctttcagtt tcagggggct
     8221 gaggaaaatc atctactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa
     8281 gaagtcatta aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
     8341 caaacatctt cgttatggga tgcatttgcc tatacattag caattcttta ctacgaaaac
     8401 tttaaaaatg cttatcgttc ggagcagggg tatattaaaa aagatgttct gataaaagat
     8461 attcttttct atctgaataa taattatcgc gaaaaaatca ctttagaaca gttatcgaaa
     8521 aaatttcgtg ccagcgtcag ttatatttgc catgaattta ccaaagagta tcgtatttcc
     8581 cctattaact atgttattca acggcgtatg acggaagcaa aatggtcact cactaatact
     8641 gaattatcac aggcagagat ctcctggcgt gtgggttatg aaaatgtcga tcactttgcc
     8701 aaactgtttt tgcgccatgt cggctgttcg cccagcgatt accgcaggca atttaaaaac
     8761 tgttttgcgg aacaagaaat cctatctgaa tttcctcaac cggtaagtct tgccggataa
     8821 atctggcggc gcagtttgtt aagaagctgc gccgcctgca gattatttgt tctgatagtc
     8881 tttcaggatc tgccgacctg ctacgtaaat cataatttcg tctgtaccgc cgccaatacg
     8941 ttcacaacgg acatcacgcc agaagcggga gacgcgcgcc tcatcggtat agcccagacc
     9001 gcccatgatt tgaatcgcat catcaatgac ttccattgcg gtacgtgcac agtacagttt
     9061 tgccagcgcc gcgctggtgc gcagtgattg atgctgatcg gcttgccatg ccactttcag
     9121 caccatgttg cgcatgttgt caatcttaat cgccatcagc gccagttttt cctggatcat
     9181 ctggttatga ccaatgggtt taccaaaagc gatacgctgg ttggcatagc gagcggcatc
     9241 ttcaaaggca cactcggcaa agccggtgct gcgcgcggcg ttgatcaggc gctccatctc
     9301 aaagttgtac atcacattga ggaaacccat tccttcttcg cccaccatgt cgctctcttc
     9361 aacttccacg ttgtcgagat agacttcgca ggtgctgagc atatgccagc cgattttatg
     9421 cagcgggtta atcttaatgc cgggcttact ggagtcgacc caccacaggg tgaaggcttt
     9481 tttgggatct ttcggttgcg gatcgcgcgc cagcaccagc atatacgggt actctttcgc
     9541 accggtaata aaggttttct gtccgttgat gtaaaccttg ccgtttttac gcgtgtaagt
     9601 ggtagtggca ctgttgttat ctgagccagc acctggttcc gtcaacgcca gggcataggc
     9661 gggatcaccc gtttccaggg tgctttctgc cgttttacgt agctgctctg cagaaccgaa
     9721 acggcgcata ctgtgaatac attgaccgtt ggtaattaaa aatgccggag caccgcattt
     9781 tgacacttcc atcagcgcca gcatttgggt gacgtaatca gcagggatac caccaaattc
     9841 ttccggcacg ccaagcatgg aaataccgtt atccgccagt gcccgcataa actcacgcgg
     9901 atatgtcccg ttttgatcgc aggtgcggaa atactcttcc ggaaagttag tcgtaatcag
     9961 ttcgcgaata ctggccagca gcagttcttg ttcttcagtt aaagaaaaat ccatcgtgat
    10021 actcctgtta atgagccgct tcaggcagga caaaacccat cgccgcatcg ataaataatc
    10081 tttcgtccat cagtttgagt tctggcgaaa tcactggggt gaaatccatt ttgtcgagaa
    10141 tatctttttg taaatcgacg ccaggggcga tttcgattaa atgcagtccg tcttctttca
    10201 gcgtgaatac ggcgcgctca gtgatataac gaacatccag ccctcgctcg agagcgattt
    10261 ttccgctaaa agtaatttcc ggtagttccc gaataaattt cttcacccgt ccttcctgga
    10321 cgatattcaa tttgccatcg gcaatttctg tttttaaact gcccgcagtt aacgtgccgc
    10381 agaaaataat tttcttcgag gtggcactga tatcaataaa tccaccggta cccatgattt
    10441 tgccattgaa tttatgcacg ccgacgttac cgtgctggtc gacttcagca aaactcaaat
    10501 aacaaacatc cagaccacca ccgtgataaa aatcaaactg ggacgtcata tccagaatgg
    10561 cacgggtatt cacgttcgcg ccaaaggcga taccctgtga agtaattcca ccaatcggac
    10621 ctgtttctac cgtcagaata aagtcatcag cacaaccttc ttcccgggcg accaggccaa
    10681 tgccgtcagc aataccgacg ccgacattcc ccaccgcgcc tttacgcatt tcgaataatg
    10741 cacgccgtgc aactaattta cgttggttta ggggcaggct aagtttggtg ctgtcatcaa
    10801 gggtgaagtc accagaaata aagcggttaa ccggcgcacc gccatatagt tggctttgat
    10861 ccggatcgac caccacaata tccaccagat aacccggaat acggacagat ttaggatgca
    10921 gtgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg ttattgtgca
    10981 ccgcctgggc aataaccaat gcgtcgagat acatcacctc atcttcaaaa gtggcgtagc
    11041 cttcactgtc gcaggtggta gcgcgaatga aagcaatatc tggcgcaatt gctttgtaat
    11101 agagatattc tttgttatca aactcgacca gtttaatcag gtcttcttta gtgacttcat
    11161 tcagtttgcc gccttgctgg cgtggatcga caaatgtccc gatgccaata tcactaataa
    11221 taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct tgtggatagt
    11281 tataagcaat aattttattt tgttctgcga gatcagaaat acgcggcgat tgtccccagt
    11341 gaccgcataa tgcccatttc accagacctt cttgcgccag cggactaata ccgcggtcgg
    11401 cgcgatcgcc aagccctgtt ggactaataa tcgataaatt acgtggcgtt tgagtctgtt
    11461 tatatttatc agcaagagca gtaattaacg tggtggcttc cagaataccg ccgccagcgc
    11521 ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt gccgacagga
    11581 ccggcactcg tccattaata cgaggtggtt ttacaggttt cattgttaat tcctgattca
    11641 ggtgtgattt tccctacgcc agatggataa ttagcgcaca gagactcacg a
//
LOCUS       DAZFKY010000148        11564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFKY010000148 DAZFKY010000000
VERSION     DAZFKY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            259..1029
                     /gene="yjjP"
                     /locus_tag="ACHKCL_003578"
     CDS             259..1029
                     /gene="yjjP"
                     /locus_tag="ACHKCL_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5764463.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1020..1493
                     /gene="yjjB"
                     /locus_tag="ACHKCL_003579"
     CDS             1020..1493
                     /gene="yjjB"
                     /locus_tag="ACHKCL_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5764464.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1600..2139
                     /gene="dnaT"
                     /locus_tag="ACHKCL_003580"
     CDS             1600..2139
                     /gene="dnaT"
                     /locus_tag="ACHKCL_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5764465.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2142..2879
                     /gene="dnaC"
                     /locus_tag="ACHKCL_003581"
     CDS             2142..2879
                     /gene="dnaC"
                     /locus_tag="ACHKCL_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5764466.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2928..3422
                     /gene="yjjA"
                     /locus_tag="ACHKCL_003582"
     CDS             2928..3422
                     /gene="yjjA"
                     /locus_tag="ACHKCL_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5764467.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3676..5967
                     /gene="opgB"
                     /locus_tag="ACHKCL_003583"
     CDS             3676..5967
                     /gene="opgB"
                     /locus_tag="ACHKCL_003583"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5764468.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6104..7126)
                     /gene="lgoD"
                     /locus_tag="ACHKCL_003584"
     CDS             complement(6104..7126)
                     /gene="lgoD"
                     /locus_tag="ACHKCL_003584"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO5764469.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7265..8179
                     /gene="lgoR"
                     /locus_tag="ACHKCL_003585"
     CDS             7265..8179
                     /gene="lgoR"
                     /locus_tag="ACHKCL_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5764470.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8394..9755
                     /gene="lgoT"
                     /locus_tag="ACHKCL_003586"
     CDS             8394..9755
                     /gene="lgoT"
                     /locus_tag="ACHKCL_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5764471.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9804..11468)
                     /gene="tsr"
                     /locus_tag="ACHKCL_003587"
     CDS             complement(9804..11468)
                     /gene="tsr"
                     /locus_tag="ACHKCL_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5764472.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         2810 a         3061 c         2811 g         2882 t
ORIGIN      
        1 actaaaaaac gcgcccagct atctattatc ctgacccgta aaggctaaaa caataaattc
       61 attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt cgctttttat
      121 caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta acctaccgca
      181 aaacaacctt gcttcatgat acaaatggat tctcattatc tgaacaatac gcagcacgtc
      241 tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg gctttgtatc
      301 cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga tgagctttcc
      361 tcacgactgg gtcgggcgct gggaatggac agcgtcgaaa gttctatctc ttcgaacgcc
      421 atagtgctga caactattaa agatgggcaa tgcctgacat cgacacgtaa aaatcacgat
      481 cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct tgcggagcat
      541 catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc attacgttac
      601 ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg taaactcaat
      661 aacggtggct gggatggtgc cgtcatcacc ttctttgcca gtacgaccgc gatgtatatc
      721 cgccagctgc tggcacaacg tcatcttcat ccacagatca acttttgcct taccgctttc
      781 gccgccacca ccatttccgg attgcttttg caacacccca ctttcagcaa tacccccacc
      841 attgcgatgg ccgccagcgt tctgctgcta gtgccgggct ttccgttgat taatgccgtc
      901 gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat cgccagtctg
      961 ctgacactgg ctacctgcgt cggcgtagtg atggcactga cgatttgggg gctacgcgga
     1021 tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat gatcctcgcc gccattcctg
     1081 cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc gttacgctgg tgtgcgctgc
     1141 ttggctcgat aggtcatggt tcccgaatga tcttgatgac cagcgggttg aatattgagt
     1201 ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg tcgcgctggt
     1261 atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc ccgggcatat
     1321 cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac agcgaaccgt
     1381 tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt gcgttatcca
     1441 tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta taaaattgcc
     1501 tcgcttaagc acacggatga gagacagcct cctctcctcc gtgtgttact ataaaagtta
     1561 tctcccttct cgttcatcgt tccatatttg agaaacagta tgtcttccag agttttgacc
     1621 ccggacgtcg ttggtattga cgccctggta cacgatcacc aaaccgttct ggcaaaagct
     1681 gaaggcggtg tggttgccgt atttgctaac aatgccccgg cgttttatgc cgtcacgcct
     1741 gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg cgcgtccggg aagcgatgtc
     1801 gatctggacg atcaactcta tcaggaaccg caagccgctc ccgttgctgt acccatgggg
     1861 aaattcgcca tgtatccgga ctggcaaccc gatgccgact ttatccgcct ggcggctcta
     1921 tggggcgtgg cgctaagaga gccggtaacc accgaagaac tggcctcatt cattgcctac
     1981 tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc aacaaaaact ggcgcgcagc
     2041 ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac gagatgtgaa tacggtcagt
     2101 gaacctgaca gccaaattcc accaggattc agagggtaac gatgaaaaac gttggcgacc
     2161 tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa acctgcattc aaaacgggtg
     2221 aagagctact ggcctggcag aaagaacaag gggcgatccg ctccgccgct ctcgaacgtg
     2281 aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc cggtattcgc ccactgcatc
     2341 agaactgctc ctttgagaac tatcgcgttg agtgtgaagg gcagatgaat gcgttaagca
     2401 aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc cagcttcatc ttttctggta
     2461 agccaggaac cggcaaaaac catctggcgg cggcaatctg caacgagctg ctgctacgcg
     2521 gtaaatccgt attgatcatc accgtggccg atattatgtc ggcgatgaaa gataccttca
     2581 ggaatagcgg taccagcgaa gaacaactgc ttaacgatct gagcaacgtt gatctgctgg
     2641 tgatcgatga gatcggcgtg cagaccgaat cgaaatacga aaaagtgatc atcaaccaga
     2701 tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat gctgaccaac agtaatatgg
     2761 aagagatgac caagttgctg ggcgaacgcg tgatggaccg tatgcgcctg ggtaacagtt
     2821 tgtgggtgat cttcaactgg gatagctacc gtagccgtgt aacaggtaaa gagtattaat
     2881 ccccgtttct ggcggtatac tgcgacacaa ttccaccttg agtaatgatg atgaaaactg
     2941 ttaaacatct tctgtgctgt gccattgccg ccagcgcatt aatttccacc ggggtgcatg
     3001 ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga acttggcaac caaaacagca
     3061 cgacacagga aggcggttgg tcgctcgcgt cattaactaa cttgcttagc agcggaaacc
     3121 aggccttaag cgcagataac atgaacaatg ccgcaggcat tctgcaatac tgcgcgaagc
     3181 aaaagctggc ttcggtaacc gatgtcgaaa acatcaagaa ccaggtgctg gaaaagctgg
     3241 gcctgaacag tgaagagcaa aaagaagaca ccaactatct ggacggcatt cagggtttgc
     3301 tgaaaaccaa agatggtcag caactcaatc tggataacat cggaacaact ccgctggcag
     3361 aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca ggggctgaac ttcatttcct
     3421 gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac gcggctctct cctgcccttt
     3481 tccctgctcc tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc
     3541 tgtttataaa ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat
     3601 ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt
     3661 agtgaggatc gcccgttgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg
     3721 atttacgcat ggaaagcggg acgtaacacc tggtggtttg cagccacatt aacggtgctg
     3781 gggctatttg tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt
     3841 attaacgacg cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa
     3901 tacattttgc cgggtatcgg cattgtgctg gggctgacag cggtgttcgg tgcgctgggc
     3961 tggatcctgc gccgtcgtcg ccatcatccg caccattttg gttacagcct gctggcgctc
     4021 ttactggcgc tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg
     4081 aaatcccagt cacgcgacgg cgacccggac tttgcggctt attataaaga gccgtcgaaa
     4141 actatccctg acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc
     4201 tattttgata acgaggcttt cccggacctc acgcctgaac tgggcgcgtt gaaaaatgaa
     4261 ggcctggatt tcagccacac gcagcagctg ccgggaacag attacacgat tgcgggcatg
     4321 gtggcttctc agtgcggcat accgctgttt gccccctttg aaggcaacgc ctccgcttct
     4381 gtttccagct tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat
     4441 cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg gtaaagatgt gttcctgaag
     4501 tcgcatggct tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg
     4561 cactatcgca acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag
     4621 tttgaagagc tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc
     4681 catcacccgg atggttttat ctctcgtacc tgtaaccgca aaaaatatga ttttgacggt
     4741 aaaccgaatc agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc
     4801 aacaaaatca aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat
     4861 ttagcgatga acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt
     4921 tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg cagtgaagcg taacacgatg
     4981 gataacggag cgacagtgct ggacattctc ggtggcgata actatctcgg acttggtcgt
     5041 agcagtttat ccgggcagtc aatgtcggaa atcttcctca atatcaaaga gaaaacattg
     5101 gcgtggaagc cggatatcat ccgtctgtgg aaattcccta aagagatgaa agagttcacc
     5161 atcgaccagc agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg
     5221 ttgcgggttt cagacaaacg cgtggaaccg ctgccagaaa gcgaatactc agcaccgctg
     5281 cgtttccagc tggccgattt cgctccacgc gacaatttcg tctgggttga ccgttgctac
     5341 aagatggcac aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag
     5401 gggcaacttg gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag
     5461 acggcattta aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg
     5521 aagattgttg ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt
     5581 gcgccagaag aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg
     5641 tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa
     5701 tttgacctgg tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta
     5761 cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg
     5821 catttcgata acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca
     5881 accaacgaag ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa
     5941 attaaagtgg tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg
     6001 acctacacac cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa
     6061 cacttccccg gacgctgcat ggcaacttta ataatctcgc ctttcagaaa gtaattacgc
     6121 ctttaattaa ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga
     6181 acggatagcg atgggttaac atcatgccag cagtgatttt tccttccgcc atcagtcgac
     6241 cgactttagc aaaatcttcc ggcgtggcgt tgcggctgcc catcatcgtc gtttcttttt
     6301 tatggaattc cggatcggag aactgcaact cacctttaaa caggccgaca aataccaccg
     6361 tgccgccgtg acgaatcaga ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt
     6421 cgatcacttt ctgcgccagc gaaccaccaa actgcgcccg cagctgcgcg tcaaaatctt
     6481 cggctgacgg gtccagcacg ggcaactcca gacgcgttgc cacatgttca cggcgcgccg
     6541 gactggtatc cgccaccacc acctgtgcgc catcggcttt agcgattgcc gccgcgccca
     6601 gaccgattgg cccggccccg accaccagca cctgctcgcc gggagcaatg gctgcgcgac
     6661 gcaccgcatg agcgctaata gcgaaaggtt caatcaatgc ggccgcctgc gggtcaatac
     6721 cgtctgcggg caaaatgttc gccaccggca cgctcaaata ctcactaaaa ccgccatcct
     6781 gatgcacgcc aatgactgaa attttttcac agcaattggt acgcccgctt ttacacgccg
     6841 ggcattgctg acaggcaaca taagggatca cagcaacttg ctgaccattt ttaagatcag
     6901 caatattttt acccagccca acaatctccc cacatatttc atggcctaaa acccgtggat
     6961 aactaaaaaa tggttgattt ccaccccagg catgaatatc ggtaccgcaa atcccgacag
     7021 acttaatttt tattaatgct tcattgtcac ccggaatagg tatctcgcgt tgtttccaga
     7081 ctaattcttt cggctgctgg caaattaaaa cattcatcgt agacatgatt cggctctcca
     7141 ttttttgttg ccttagatat caatgaaaat gaaaaatggc agcggaacgg aaaatctgtt
     7201 ttgtgaaaac acacgcataa aaatgtttta aatcgggttt taatggactc acaaggagga
     7261 aaatatgagt cgttcacaaa atttacgcca caatgtgatt aaccaggtca ttgatgatat
     7321 ggcgcggggt catatccctt ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa
     7381 catcagccgc actacagtgc gtcatattct cagccactta cgcgaatgcg gcgtcttaac
     7441 gcaggttgga aacgactatg ttattgcgcg taaacctgac catgatgacg gtttcgcctg
     7501 tactaccgct tcaatgagtg aacaaaacaa agtgtttgag caggcttttt tcacgatgat
     7561 aaaccagcgc cagttacgcc caggggaaac tttttctgaa ctgcaactgg ctcgggccgc
     7621 gggtgtcagc ccggtggttg taagagaata tcttttaaaa ttcggccgtt ataatcttat
     7681 tcaaagcgaa aagcgcggcc agtggagcat gaagcaattc gatcaatcct acgctgagca
     7741 gctgtttgag ctacgcgaaa tgctcgaaac acattcactt cagcactttc tcaacctgcc
     7801 tgatcatgat ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg
     7861 tgacaacatc ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt
     7921 actgctttca gctgccgaca atatcttttt tgatcaatcg cttgagatca tctcagtcat
     7981 ctttcatttt cactatcaat gggacgaaag cgatcttaaa cagcgcaata tcattgccgt
     8041 cgatgagcat atgaccattc tcagcgctct gatttgccgc agtgaccttg atgccaccct
     8101 tgcgctccgt aaccatttga attcagcgaa acaatcaatg attcgatcca ttaatgagaa
     8161 cacgcgttat gctcattaag tacggattaa aaacagcaaa ctattagcgg gtgcacggtt
     8221 ttatcaaaag ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa
     8281 aaatgttttt gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atgtcaacct
     8341 tgcgtaatgc acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa
     8401 aagaaaatat caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc
     8461 ccgtgccacc agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg
     8521 ccatgttgtt attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg
     8581 tagcaaattt aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc
     8641 tctccgtgtt ttcactcgct tacggcattg cgcaacttcc ttgcggccca ctattggatc
     8701 gtaaaggccc acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcca
     8761 tgtctggcat ggtgcacagc tttacgcagt tcgtgttggt gcgtatcggt atggggattg
     8821 gtgaagcgcc gatgaaccca tgcggtgtaa aagtcattaa cgactggttc aacatcaaag
     8881 agcgcggacg cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc
     8941 caccgattct ggcggcgatg atgctggtga tgggctggcg cgggatgttt attaccattg
     9001 gtgtactggg gatttttctc gccatcggct ggtatatgct ctatcgcaac cgcgagcacg
     9061 tagaactgac tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag
     9121 atccgctcag ttttgccgaa tggcgcagcc tgttccgtaa ccgcacaatg tggggaatga
     9181 tgctcggatt cagtggcatc aactacactg cgtggctgta tctggcctgg cttcctggtt
     9241 acctgcaaac agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt
     9301 tcctgtttgg tgctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag
     9361 ggggaatggc tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg
     9421 ccgcctttac gctgatagta ccacaagcga caacatccat gatggcggtt ctgctgattg
     9481 gcatggcact gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg
     9541 cagttgcttc tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc agcttcatct
     9601 gcgcctcttt cgcgccgatc attactggtt ttattgttga tacaacccac tcattccgtc
     9661 tggcactaat catttgcggt tgcgtcaccg cagcgggagc actggcgtac atcttcctgg
     9721 ttcgtcagcc gatcaacgac ccgcgtaaag attaaactcc ctatgcccga taacattttg
     9781 cttatcgggc atttttatgg cgattaaaac gtttcccagt tctccccgct atctgccact
     9841 gccattttac gcggcgtagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc
     9901 tgttgctgct gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc
     9961 tcttccaacg cggcggcggc agcggcagac tcttccacca gcgcagcgtt ctgttgagtt
    10021 acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca
    10081 gaagcagaag ctatttcgcc cataatgtcc gtcacgcggg tcacagcact gacaatctcc
    10141 gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt ccccaccgag
    10201 tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tacgctgggc cagattacgc
    10261 acttctcccg caaccaccgc aaaaccacgt ccttgctcac ccgcacgcgc tgcttcaacc
    10321 gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata
    10381 tcggcgattt tctgcgaact ggtggagata tcacgcatag tctgcaccac gttatctacc
    10441 actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccaggtggct ggcctggcgc
    10501 gcattctcgg cgttctgttt caccgttgcg gtgagttgct ccatgctggc tgccgtctct
    10561 tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt
    10621 tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc
    10681 agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc
    10741 acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga
    10801 ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt
    10861 acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg
    10921 ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg
    10981 aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct
    11041 aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct
    11101 gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc tgcccagttt
    11161 ttttccgcct gtttcaacga aatactggca ctctgcatca gctcagcaac ggttgaaccg
    11221 ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc
    11281 gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt
    11341 aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc
    11401 agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat cttgatacgt
    11461 tttaacatgg tttctctttc ctgtggaccg atgcaaaatt ttcggcctga aatgagaaaa
    11521 ctttataggt agcgacttga aactggttaa ttttacctca cttt
//
LOCUS       DAZFKY010000149        11491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFKY010000149 DAZFKY010000000
VERSION     DAZFKY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..1138)
                     /gene="lptG"
                     /locus_tag="ACHKCL_003588"
     CDS             complement(56..1138)
                     /gene="lptG"
                     /locus_tag="ACHKCL_003588"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5764473.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(1138..2238)
                     /gene="lptF"
                     /locus_tag="ACHKCL_003589"
     CDS             complement(1138..2238)
                     /gene="lptF"
                     /locus_tag="ACHKCL_003589"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5764474.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(2301..2351)
                     /gene="ytgA"
                     /locus_tag="ACHKCL_003590"
     CDS             complement(2301..2351)
                     /gene="ytgA"
                     /locus_tag="ACHKCL_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5764475.1"
                     /translation="MSIIILATISTTQENK"
     gene            2505..4016
                     /gene="pepA"
                     /locus_tag="ACHKCL_003591"
     CDS             2505..4016
                     /gene="pepA"
                     /locus_tag="ACHKCL_003591"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5764476.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4370..4813
                     /gene="holC"
                     /locus_tag="ACHKCL_003592"
     CDS             4370..4813
                     /gene="holC"
                     /locus_tag="ACHKCL_003592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5764477.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4813..7668
                     /gene="valS"
                     /locus_tag="ACHKCL_003593"
     CDS             4813..7668
                     /gene="valS"
                     /locus_tag="ACHKCL_003593"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5764478.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7723..8919)
                     /gene="yjgN"
                     /locus_tag="ACHKCL_003594"
     CDS             complement(7723..8919)
                     /gene="yjgN"
                     /locus_tag="ACHKCL_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO5764479.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            9112..9615
                     /gene="yjgM"
                     /locus_tag="ACHKCL_003595"
     CDS             9112..9615
                     /gene="yjgM"
                     /locus_tag="ACHKCL_003595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5764480.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9661..10077)
                     /gene="rraB"
                     /locus_tag="ACHKCL_003596"
     CDS             complement(9661..10077)
                     /gene="rraB"
                     /locus_tag="ACHKCL_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5764481.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            10239..11243
                     /gene="argF"
                     /locus_tag="ACHKCL_003597"
     CDS             10239..11243
                     /gene="argF"
                     /locus_tag="ACHKCL_003597"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5764482.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(11300..>11491)
                     /locus_tag="ACHKCL_003598"
     CDS             complement(11300..>11491)
                     /locus_tag="ACHKCL_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764483.1"
                     /translation="KDVIEEKLNNELKLRFRMMQTILKSEVNVSPFIDQQRLNTLNPP
                     ENLRIAIEKFGWKKKTITA"
BASE COUNT         3033 a         2938 c         2897 g         2623 t
ORIGIN      
        1 acactttgct aacaataata aagggagctt tcgctccctt tattcgttca ttcggttacg
       61 attttctcat taacagccac aggctgatta agaagaagct ggcgcttggc aacagtgcgc
      121 cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc gaagatctgg tccagtacgt
      181 agaagacaaa accgaaactg ataccggtga ccacacgcac gcccatcggt acgctacgca
      241 gtgggccaaa gatgaacgac agcgccatca gcatcatcac cgccacggat agcggctgga
      301 agattttgct ccacatgttg agctgataac gtccggcatc ctgaccgctc gacttcagat
      361 acttcacata gttgtgcaaa ccgctgatag agagtgcatc cgggtccagc gccaccacgc
      421 ccagtttgtc cggcgtgagg ttggttttcc aggtgccgct caccgtctgc gaaccggtaa
      481 tctgtttcgg attggtcaga tcagattcat caacctgcga caaacgccag actttatgtt
      541 ccgggtcaaa cttcgcagta gcggcatagc gtacggattg cagacgacga ttctcgttaa
      601 aggcataaat gctgatgcca cctaactctt cgtcaccttt aacccgctca atgtagacga
      661 agttgttgcc atctttcgcc cataagcctt gctgggtaga gagcaacgag ccgccgtaca
      721 tcgcctgcgc acggtagtta cgcgccatct gctcgccctg cggcgcgacc cattcgccaa
      781 tcgccatcgt cagcaagacc agcggaatgg cggttttcat caccgacagc gccacctgca
      841 tacgggtaaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa
      901 gaccaagcaa cgccccaagc agagccgcca tcgggaagaa tatttgcaca tctttcggca
      961 cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc ccggcttttt
     1021 tcaactgatc gacaaacttg ataatgcccg acagcgacac cagcatgaac agcgtcatca
     1081 tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa ggttgcatca
     1141 caccgctcct ttacgcgaaa aactggcgcg caggcggcgg accggcacgg tgtcccaaag
     1201 gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc acagcgtcgg
     1261 gtccagctta cctttaccgc cgttcgattt cagggaggtc tggatcagga agaaaagtag
     1321 atacagcagc atggctggca gcatcgacag tacgcgtccc tgacgtgggt taaccacgct
     1381 cagcggtacg accataagtg ccatcataaa cacggtgaat accaacgtga tacgccagtt
     1441 cagttctgcg cgagcacgat cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc
     1501 ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa
     1561 atccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc cctggttgag
     1621 agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac
     1681 agaaggacgt gcattacctt ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct
     1741 gccgtcaacg ctttcgatga acagcaccga actgccatta gtcgcttgct ggaattgccc
     1801 ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt catcctgatg
     1861 acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg ccgtgaatac
     1921 cgcaaggatc attgccgctt tcaccagaac cgctttgctc aggccgcagg catgcattac
     1981 cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga ggaacaggct
     2041 taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg agagcaccag
     2101 attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact tttgacagaa
     2161 gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct cccgcaccag
     2221 atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc aggattttag
     2281 cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt cgctaagata
     2341 attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc cgtaataacg
     2401 ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct tgcaattcta
     2461 tctgtagcca ccgccgttgt ctttaagatt caggagcgta gtgcatggag tttagtgtaa
     2521 aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac
     2581 gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac atcagcgccc
     2641 tgctacgtcg gggcgaactg gaaggaaaac cggggcagac attgttgctg caccatgttc
     2701 cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt gagctggatg
     2761 agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat actggctcaa
     2821 tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac tactggaaag
     2881 tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag tttcgatcag ctgaaaacga
     2941 acaagagcga accgcgtcgt ccgctgcgta agatggtgtt caacgtgccg acccgccgtg
     3001 aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc gggattaaag
     3061 ccgcgaaaga tctcggtaat atgccgccga atatctgtaa cgccgcttac ctcgcttcac
     3121 aagcgcgcca gctggctgac agctacagca agaatgttat cacccgcgtt atcggcgaac
     3181 agcagatgaa agagctgggg atgcattcct atctggcggt cggtcagggt tcgcaaaacg
     3241 aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca cgcccaatcg
     3301 tgctggtggg taaaggttta accttcgact ccggcggtat ctcgatcaag ccttcagaag
     3361 gcatggatga gatgaagtac gatatgtgcg gtgcggcagc ggtttacggc gtgatgcgga
     3421 tggtcgcgga gctacaactg ccgattaacg ttatcggcgt gttggcaggc tgcgaaaaca
     3481 tgcctggcgg acgagcctat cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg
     3541 ttgaagtgct gaataccgac gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg
     3601 ttgagcgttt tgagccggaa gcggtgattg acgtggcgac gctgaccggt gcctgcgtga
     3661 tcgcgctggg tcatcacatt accggtctga tggcgaacca taatccgctg gcccatgaac
     3721 tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt
     3781 atcaggaaca actggagtcc aattttgccg atatggcgaa cattggcggt cgtcctggtg
     3841 gggcgattac cgcaggttgc ttcctgtcac gctttacccg taagtacaac tgggcgcacc
     3901 tggatattgc aggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg
     3961 tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa gagtaattgc
     4021 gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc tacacagcac
     4081 tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg ttattgtcgg
     4141 atgcggcatg aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc tgataagacg
     4201 cggcagcgtc gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg ccttatccga
     4261 cctacacaca gcactgaact cgtaggcctg ataagacaca acagcgtcgc atcaggcgct
     4321 gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata tgaaaaacgc
     4381 gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact
     4441 ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca tcgcctgtga
     4501 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
     4561 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
     4621 gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
     4681 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
     4741 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
     4801 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
     4861 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc
     4921 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
     4981 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
     5041 gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
     5101 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
     5161 tggaaagcgg aatccggcgg cacgattacc cgtcagatgc gccgtctcgg caactccgtc
     5221 gactgggaac gtgaacgctt cacgatggac gaaggcctgt ccaatgcggt gaaagaagtt
     5281 ttcgttcgtc tatataaaga agacctgatt taccgtggca aacgcctggt aaactgggac
     5341 ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
     5401 atgtggcaca tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg taaagattat
     5461 ctggtggtcg cgactacccg tccggaaacc ctgctgggcg atactggcgt agccgttaac
     5521 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
     5581 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
     5641 aaaatcactc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca cgcgctgccg
     5701 atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt gttcgatacc
     5761 aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca gaaactcgag
     5821 cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
     5881 attaaaccgc acgacctgac agttccttac ggcgaccgtg gcggcgtagt tatcgaacca
     5941 atgctgaccg accagtggta cgtacgtgcc gatgtactgg cgaagccggc agttgaagcg
     6001 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
     6061 atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca ccgtatcccg
     6121 gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagaaga agtgcgcaag
     6181 gaaaataacc tcggtgccga cgttgccctg cgtcaggacg aagacgttct cgacacctgg
     6241 ttctcttctg cgctgtggac cttctctacc ctaggctggc cggaaaacac cgacgcactg
     6301 cgccagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt cttctggatt
     6361 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
     6421 ccgttccaca ccgtttacat gaccggtctg attcgtgacg acgaaggcca gaagatgtcc
     6481 aaatccaagg gtaacgttat cgacccgctg gatatggttg acggtatttc gctgccagaa
     6541 ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa aatccgtaag
     6601 cgcaccgaga agcagttccc gaacggtatt gagccgcacg gcaccgatgc cctgcgcttc
     6661 accctggcgg cactggcgtc taccggtcgt gacatcaact gggatatgaa gcgtctggaa
     6721 ggctaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacacg
     6781 gaaggtcagg attgcggctt caacggcggc gaaatgacgc tgtcgctggc ggaccgctgg
     6841 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
     6901 ttcgatatcg ccgcaggcat tctgtatgag tttacctgga accagttctg tgactggtat
     6961 ctcgagctga ccaagccggt aatgaacggt ggcaccgaag cagaactgcg cggtactcgc
     7021 catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
     7081 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
     7141 atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac
     7201 accgaatggc tgaaacaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc
     7261 gcgccgggca aaccgctgga gctgctgctg cgtggttgca gcgcggatgc agaacgtcgc
     7321 gtaaatgaaa accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat caccgtgctg
     7381 cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc agagctgctg
     7441 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg
     7501 gcgaagatcg aaggcgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc
     7561 gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg ctatgcggaa
     7621 gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt
     7681 gatgaaaacg aaggccggag catgctccgg cctttttatc tcttacagaa atggtaaaga
     7741 aggcataatt ccccgtgaaa tactggctaa gaagccttta tctggttgtt cttctttatt
     7801 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
     7861 catccatatt ttcagcagtg gataagccag accgcccgta atcccggata tcaccaccaa
     7921 cgcacacatg cgataaagca taccgtggta tgttaaagtt gagcgaaaac gaatacgccc
     7981 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
     8041 gctaacagca atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc
     8101 cataaattgc tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt
     8161 taatatttga tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa
     8221 tgcgaggatg gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat
     8281 accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa
     8341 aataccaatg gaaacaatac caactattgc catcagaacc acagatacaa taacactact
     8401 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
     8461 cattaaaatc ggcaagaaaa aggttaccca ccagacccct ttcatagagc aattaaaact
     8521 aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat
     8581 aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaataagcg gcatatctgc
     8641 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac
     8701 attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcatagag
     8761 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
     8821 caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga
     8881 atgggacgga acatccattt cattaataac ttgagccata aaaattccct gaattaaaaa
     8941 atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct
     9001 tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag
     9061 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
     9121 attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc
     9181 cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc
     9241 gccgatccga atcttgacga gctgtatcaa gtgtatagtc agccgggtca tgcatattgg
     9301 gtggttgagt acgagggtga agtggtcggc ggcggtggga ttgcgccatt agcaggtagt
     9361 gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg cggcaaaggg
     9421 ctggcaaaaa aactggcctt aaaggcgatg gaggaggcaa gagaaatggg cttcaaacgc
     9481 tgctatctgg aaacgaccgc ttttttgaaa gaagcgattg gtttgtatga acatttaggt
     9541 tttcaacata tcgactatgc acttggctgc acgggccatg ttgattgtga agtgcggatg
     9601 ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt aatcaattaa
     9661 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc cgtcttcgcc
     9721 gttgggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
     9781 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
     9841 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
     9901 ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca gatcgtctgc
     9961 ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc
    10021 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
    10081 tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc attgcctcca
    10141 cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca tataaattga
    10201 attttaattc attgagacgt tagccacagg agggatctat gtccgggttt tatcataagc
    10261 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
    10321 cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact ggtaaaaaca
    10381 tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa gttgccgcat
    10441 atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt ggtcataaag
    10501 agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg
    10561 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
    10621 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
    10681 atttacccgg taaaccattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
    10741 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg
    10801 cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgcc ctggcacaac
    10861 aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggcgttgaa ggtgctgact
    10921 ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agaaaaatgg gctgaacgca
    10981 ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg
    11041 aggtcaaatt cctccactgc ctgcccgcgt tccatgatga ccaaacgacg cttggcaaga
    11101 aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat
    11161 ctgccgccag cattgttttt gatcaggcgg aaaaccgcat gcacaccatc aaagcggtga
    11221 tggtcgcgac actcagtaaa taattgatat cgctataaag atgccgggaa atacagaaac
    11281 atcccggcgt caaattattt tatgcagtga tagttttttt cttccagcca aatttttcta
    11341 ttgctatacg taaattttcc ggaggattta gtgtatttaa acgctgttgg tcaataaatg
    11401 gcgatacatt aacctccgat ttcaagatag tttgcatcat tctaaaccta agttttaatt
    11461 cattattaag tttttcttca ataacatctt t
//
LOCUS       DAZFKY010000150        11452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFKY010000150 DAZFKY010000000
VERSION     DAZFKY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            76..981
                     /locus_tag="ACHKCL_003599"
     CDS             76..981
                     /locus_tag="ACHKCL_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5764484.1"
                     /translation="MRGKIAKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1015..1617)
                     /gene="cobO"
                     /locus_tag="ACHKCL_003600"
     CDS             complement(1015..1617)
                     /gene="cobO"
                     /locus_tag="ACHKCL_003600"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5764485.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1627..3279)
                     /locus_tag="ACHKCL_003601"
     CDS             complement(1627..3279)
                     /locus_tag="ACHKCL_003601"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5764486.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3387..4667)
                     /locus_tag="ACHKCL_003602"
     CDS             complement(3387..4667)
                     /locus_tag="ACHKCL_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO5764487.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4828..5145)
                     /locus_tag="ACHKCL_003603"
     CDS             complement(4828..5145)
                     /locus_tag="ACHKCL_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO5764488.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5142..6512)
                     /locus_tag="ACHKCL_003604"
     CDS             complement(5142..6512)
                     /locus_tag="ACHKCL_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO5764489.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6516..7757)
                     /locus_tag="ACHKCL_003605"
     CDS             complement(6516..7757)
                     /locus_tag="ACHKCL_003605"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO5764490.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7757..9202)
                     /locus_tag="ACHKCL_003606"
     CDS             complement(7757..9202)
                     /locus_tag="ACHKCL_003606"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO5764491.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9221..10609)
                     /gene="glmL"
                     /locus_tag="ACHKCL_003607"
     CDS             complement(9221..10609)
                     /gene="glmL"
                     /locus_tag="ACHKCL_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO5764492.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10609..11058)
                     /gene="glmS"
                     /locus_tag="ACHKCL_003608"
     CDS             complement(10609..11058)
                     /gene="glmS"
                     /locus_tag="ACHKCL_003608"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO5764493.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2734 a         2844 c         2863 g         3011 t
ORIGIN      
        1 gctgctttgg ttaccattac ttcgtaataa gtaatgtatt tttgatggtt taaactattt
       61 ttaagggacg gcattatgcg agggaaaata gctaaaacgg aactgctggt gacttttgag
      121 gtcgtcgcac gtcatgaaag ttataccaga gcggcagagg agttagcgtt aacccaaagt
      181 gcagtattca gacaagtaag tgcgctggag gaatttttac gtactccgtt attttcacac
      241 tctaaaaaac gcatttttct taatgataca gggaagtatt acctggggat agtaaaagaa
      301 acattaaata agctggaacg cgataccaat accattatga cctggcagcc tacggttcag
      361 gttattgaac ttgccgttaa tcctactttt agtacccact ggttaatccc aaatttacat
      421 gaattcacaa agttacatcc cgatattatt gttaatattc attctttagc taataacggc
      481 gattttctta atcgggagta cgatgctgtc attatgcgcg aaaatttttg cgcgccctgg
      541 gcggaagtag aatatctctt tgaagaggag attttgccgg tatgcagcgg cagtttgctt
      601 gcaatgtccg atcaaaaact gtcagtggca gaactgctta ctgagttacc acttctgcat
      661 caaagcaccc gtattactgg ctgggaagag tggtttgctt tatctggcgt tagcagtccg
      721 ctggtcaaca acggaccacg tttcgatttg ctttcaatgc tgattgccgc cgtgcgctct
      781 aatctcggcg tggcgctgtt accacgtttc gctattcaac atgatttgga tagtggcgat
      841 atggtgattc cctgtgatgt gcctattcgt accggtaatc gttttattat gacgtggcag
      901 gaagagaagt ctgactcacc gcacttgcag caatttcgtg aatggttgct ggcgaaatcg
      961 gtcgtaccgc aggagatgta aaacctgcgc ctggcgggat gccaggcgca gttattacca
     1021 gtcgataccc ggctgggcct gaatgccgtt atcaaaagca tgtttgacgg ggcggatttc
     1081 gctgaccgta tctgccattt ttaatatctg actgtggcaa ccgcgtccgg tgacaatgac
     1141 actttgttgt gccgggcggt tttgcagaga agcgatcact tcttcggtat ccagataatg
     1201 gtaagccagc atataggtca gctcatcaag caccactaag tcgtaacgct tgtcagccag
     1261 catccgtttg ctttccgacc agacctcttt cgccgcgtcg atatccgctt gccggttctg
     1321 tgtctcccag gtaaaaccgg ttcccataat atgaaactca acccccagcg gctggagaag
     1381 gttgtattcg ccgttgtccc attgcccctt gatgtactgc gcaaccccca ccgttttgcc
     1441 atgacccacc gcgcgcgtca cagtaccaaa cgcggcagtg gatttccctt tgccgttgcc
     1501 ggtgaaaaca atcaagatcc cttttttctc tatcgccgcc gcgacgcgcg tatcgacctg
     1561 ttccttcaat ttctgttggc gctgcacatg ccgttcagtt gaaattcgcg cttccattca
     1621 ttttccttaa tgtcccgcag ggcaactggc gcagcggtgt tgctgctcaa actgactaaa
     1681 gaaattattg cctttatcat ctaccaggat gaatgcgggc agattttcca cttccatcat
     1741 ccacaccgct tccataccca gctccgggta ttccaggcaa cgcagacttt tgacatattc
     1801 ttgcgccagt aacgcagctg caccgccaat acttcccaga ttgaagccgc catgtttgtg
     1861 acaggcatcg gtgacctgct ggctgcggtt acctttcgat aacatcacta gactgccgcc
     1921 agccgcctgg aaggtatcga tataaccgtc cattcgaccg cccgtggttg gacccagtga
     1981 accgcaggcc atgttctccg gtgttttcgc cgggccagcg taatagacaa tatgatgctt
     2041 caggtactcc ggcatcggtt cgccgctatc cagccgcgct tttattttgg cgtgggcgat
     2101 atcgcgcgcc actacgatgg gaccacttaa cgacacgcgg gtgcctactg gtaatctggc
     2161 gagatcctgc atcacgtcac gcaacggacg gtttagatcc agttgtacat gttgcgcgtg
     2221 attttcttcc cgcagtgagg ctggaatata ttgccccgga ttgtgttcga gtttttccag
     2281 ccagataccg tgtttgttaa tttttgcttt gatattgcga tcggcagaac aggagagcgc
     2341 catagctatc gggcaagagc caccgtgacg cggcagacga atgacgcgaa tatcatgagc
     2401 gaaatattta cccccaaatt gcgcaccaat accaaattgc tgactggcct cgagcaacac
     2461 tttttccagc tcaatatcac ggaatgcttg tccctgctcg tttccgctgg tgggcaaatt
     2521 gtcgtaatat ttcgttgagg ccagcttggc aatttttaag gtttgatcgg ctgaaagccc
     2581 tccgacgaca aaggcgatgt ggtaaggcgg gcaggcagcg gtgcccagcg atttcatttt
     2641 ttcgattaaa aatgcggtca gtttttctgg ttgcaataag gatttggttt cctgataaag
     2701 cgcggcttta ttggcggagc cgcccccttt attaacacag agaaaatggt actcatcacc
     2761 agcgacagcg ctgatatcaa tctgcgccgg gagattggtt tgcgtattga cttcggtata
     2821 catatccagc ggcgcatttt gtgaaaagcg cagattattt tcctggaaag tactgtaaat
     2881 gcctttactt aaggcttcgg cgtcgttacc tccggtccat atctgttgcc ctttactggc
     2941 aacaatagtt gcggtgccgg tatcctgaca gttgggtaat accccttttg ctgagacttc
     3001 agcattacgt aataactgca acgcaacgta tttatcatta ctgctggcct gctgatcgtt
     3061 aagaatgctg gcgacctgtt gcaaatgagc ggaacgtaag aaaaaagagg cttcataaaa
     3121 tgcctgccgg gcaagcagcg ttaatgcttc aggagcgatt tttatcactt cttcgccgtc
     3181 taattccgtt actgtaatat gttgatcgct aattaatgta tattctgtac cgtctttatt
     3241 ttgtagaaac ggttcttgcc agataaatgg tttggacata tttcactttt caccttaaac
     3301 gttagtgtta cttaattgcc gtctaacttc atgatccggc ttataaaaat gcgtagtcat
     3361 tggcttctga ttgcttaaga tccccctcag ggcagagcaa cgttcgattc attgggtttt
     3421 aatgctttac cccgacagcc ctcgccgaaa tgttcgcctt cccaccgact caccatcgca
     3481 gcgcccattg cgttgctcat gacgtttgtc gcggaacgcc ccatatcaag gaacggatca
     3541 acgcccatca gcaagatcag ccccgcttcc ggaatattga actggttgag cgtggcggcg
     3601 ataaccacca ttgaggcgcg tggtacgcca gccattcctt tcgaggtcaa catcaggatc
     3661 aacagcatgg tgatttgctc accgatggat aaatggatat tgcaggcctg ggcgatgaaa
     3721 actgtggcga aggagcagta ggccattgat ccaacgagat taaatgagta gccaatgggt
     3781 aagacaaagc tggcaatttt gggggaaacg ccaaattgct ccagtttttc aagcgttccc
     3841 ggaaaagccg cttcagaact ggatgtggta aatgccagca gggcgggttc cgaaagggcg
     3901 cgggtcaggc gtctgatgca ggggccgaca taaacgatgg ccagaccgat aagcagtacc
     3961 caaagtaata acatggtgaa ataaaattca cccataaaga tcccggcgct caccataact
     4021 gccagtcctc gttcagcaat caatgctgaa atagcagcga atacggtcag gggagcgaag
     4081 agcatgacgt agccagtgag ctttaacatg gcatgtgcca gcgaatctaa ggcgtgaacg
     4141 atggcgctgc ctttctcgcc aatcgccgtc aggctacagc cgaggaaaat tgagaacacc
     4201 acgatttgca aaatttcatt gtgcgccatg gcatcgacaa tgctggtggg gaaagcatgc
     4261 gaaataaata cttttagcgt aaagggttct gccgcgacca ctccggtggt ttcggcaccg
     4321 tgtgcaacaa aattaattcc tgtacctggc atgaagaaat ttaccgttat caagcctaag
     4381 gcaattgaca gcaatgaggc gcaaataaat aaaaagagtg ttttagaaaa aatacgacca
     4441 agggctttgg catcgcccat tttagcaata cctaccacca gggtagagac cactaacgga
     4501 gcgataatca tttttatcag tcgtaagaaa atcgtcgtga atatcgatat ttcttgcgca
     4561 tagagttttg cggtttccgg tgaagcagtg ttattgagca ctacgccgat aatcattcca
     4621 agtaccagcg ccaaaagaat catcgtggtt aaacttattt tcttcatttt ttcctccatc
     4681 aggtgcacgg cgataaatca gaatcaggag aagtagatgc ctgaatagag attcaggcat
     4741 ctacctgcta ttacaacctt acaaatagct gaagtgttga actgcaataa gtgacgggat
     4801 gtcagttggc taaaagtatt cggattttta caaatcgaaa ctcatattta ataaaggtgc
     4861 atgttgctgt gcaccataga catcattgtc acctacattc ccggagataa tatctcgtgg
     4921 catgacgatt tttactgcgt tagaaggatc aaaccagaca atacgatgaa taaagtcagg
     4981 ttcaacatga tataagcgtg caatcagttc tggggttaat tgccctgatg atttaactcg
     5041 ctcatagttt tctttcgttt taaataaaat atctaaaacc agttcgtaag gtccggcatt
     5101 tttcgaacga atgacttgcg ccagtgagac aattgactgt ttcatgcctg aactccttct
     5161 ggcgtcacct gttcaatatg gaaatcaaaa cgcagagcgt cgttggcttc aatcaggtga
     5221 tatatggaaa attcgtatac cggcccgccc tggatatcag aaggggagaa cgggaacgcc
     5281 agattacctg cggtagcgat gcggttttca tagccgtagt gcagcatggt agagcgcacc
     5341 agcgagcaaa cgctgttggc aatttcctgg gtcggtgcaa ctacatcaag gacaatcccc
     5401 agctcatgcc cggcagtttg catcggttca tggtctccca tcaccccgtt tttgccgtac
     5461 aggtggaaat tgatgcgaat gctgtcatcg tcgagcgaca ggttacgcga aacgctggtt
     5521 ttgacttcat cgatgatttt atcgatcccg gcgatcataa tcgggtcgcg cgttccggcg
     5581 atggtcagac agcggaagcc cacccgtcgt gcaccttcca gtttcagggc atacggcgtt
     5641 tcttcatgct tagaaccgct gacgtagact tcgccgtcat ttactgcttt aaatgtgcag
     5701 cctttcaggt tcaacacgcc gccaggtcct ggcaggaagt agggatcgga tttctcatac
     5761 agtgtgtgtg cagctgctga cgtttcggta aatttacgct tcggattaaa tgttttcagc
     5821 gtaaagccgt tgtcatcaat gatgcccatc gcacagtcag agcctgagcc gggcgttgcg
     5881 gcgattgccg cgcattcaag gattttccca caatgcagcg ccagaccttc atcaaagcct
     5941 tgcatgatcg ggagcgcagc aaagcaggcc gggtcgtaag cgcgcccgcc cagcaccact
     6001 tgcgcgcgtt ctttcagcgc ccgctggaag ggttcgatgc ccatttgcgc gacgatatac
     6061 gtactttcgt caatcgcgtc gtgggttaac ggcgggacaa aatccagcgc cgtgattttg
     6121 ccgttatcca gcgcctggtg gacgatggct ttgtcaacgt ctgccgggat caacgccatc
     6181 gagaacgaga ggtgctcttc ccgggcaatc tcatgaatta tttctcggca ccactccagg
     6241 tgtggtgcgg caccagaacc gccagccgtt ccgatcacca ccggtatatt attttgcacg
     6301 cctgcggtga tcatatagcg cagatcgcgc ttgacccctg cgcgatcggt aaacggtttg
     6361 cctgccccga ggtagtgggg gcctggatcg gaagagcctg catcaacggc gatcagatcg
     6421 ggcgattctg ccatcgcttt gcggaagctc tcttccggga atccataccc gaggattgcc
     6481 gttggcgata agactttaaa agaacgagcc atttgttaat ccttagcctg caacagcgcg
     6541 atagtacgat tcatttcgtt gaagacgata tcgaggcctt cgtcaaagcc catccctggt
     6601 tttaccagca tacgcatggg gcgagcggca agggcgacgt ggacacaggt gcgggcactg
     6661 acatcagttt cattgcaggt gccgccctgg tacgcttcca tgctgtggct gttgcagtaa
     6721 agaaccgcat cgacgatgtt gtgaatactg cccagatccg gggttttgat ttgcaccatg
     6781 tgacaactgg cagcatcagt gaaatcaaca atatcctggt aggtgttaca ccattcatcg
     6841 gccacaattt tcacgccgga accgaggcgc gtcagctctt tggtaatcgc ggtcagcagg
     6901 cgaatttgat cgggcttgtt accggcatcg accggccctt cgatgtagag cggcaggcct
     6961 tgcgcttctt tttccaggct ggcgatgtat tgcgcacagc gaagcggatc catatcgaag
     7021 atcagaccga tagtgccgta tacatcgatg tgcagggtag ggtggtagcg tgcgctggtg
     7081 cgcttgctta gaatgcgatc cgacaaccag cggacatatt cgcgcagttt ttcaccttta
     7141 aagcccagct tctcttcgac gttattaatc agcgcatggg gcagcacgtc gatgccttta
     7201 aggatcatct tatcgacggc gatatatcga tcgtcgccgc tctgaccaaa taatggaatg
     7261 gattccgcca cgcgtggcaa ctgccattca tcacagacca cttcagtttt cagacggccg
     7321 gttgccagcg cggtagcatc aagcagcgcc tgtgataatc cgtagcgcac ggcggtatgc
     7381 agcaagttgc cgtcaatacg caatttgtcg aagaaacggg cattcggcag gaaagcatcc
     7441 acatcgcggc ctaccagtaa tggcttgata tggtcgttga ggaacggaat aaagtgctct
     7501 gcgaggaaca gtggatcgcg accgcctgcc ccggaatact gtacggcagt acagtcacct
     7561 acggcgaccg cgccgttttc cagaatcaac tgtaccgata cgcactcccc ggcctgacgt
     7621 acggcgttaa acccctgtgt tactggctcc ccggtataaa aaaagccgtc atgacccgct
     7681 ccgtttttta tcgcctgctg gtcatcgaaa tagaatgagg agtagccagc ggtgaacaga
     7741 gcctgtttta ttttcattat ggtcttccta ttaatttact gtgggataca gcattgatgt
     7801 catctacaac catctggaat gtcggttggc gtccttcaca gcgtgcgcgt tccgcaacgt
     7861 aatcgtggtg gaggtcgaga atatcttttg gcaatggaac ggctccggct tccagtacgc
     7921 gtatcgcgcc ggtgttgtca cggaccggaa gaattttgcc cgcgttgcag gcggctggcg
     7981 caaacggcac atccagtacg cccgcttcaa atgccagcac cgttccgcgc gcaatatcac
     8041 cattacccag ttcgaacact ttattcagta ccgcgcgaac ctcgctctta atcagttcaa
     8101 tttccagttc aaccgcaggg cacggcggga atttctgctc attgaccatg ttgagcattt
     8161 ggcgggaggc tttcagcccc tgaatgttgg cagcggcagt cgggataccc caggcttcgt
     8221 gcggactttt ggtgatgact ttggtggcac cagacatccc cgcgacggct gcgccccagg
     8281 agataatagc gaacgctttc gattcgtctt ccggaaagcc gcccatccac tggtgaaaca
     8341 cggtactcaa ttcatagtcg gtatagccat agctctgaaa atattcgtgt gcaagttcac
     8401 gcagagactg gatcgccgca acatcctgcg tcaggctgcc gacctggcca taaccaacgg
     8461 tgatagattt cacaccctgt tccagcgcca gcaagccttc gattatcgcg atagagtgcg
     8521 agataaacgg cgggatcagc gtacccgtta gtgggccaaa cggttcgcgg ttgatgcgga
     8581 tcccgtgttc ttcatacatc cccattaaac ggtcgcagta ctgccagtcg cggatcgatt
     8641 tttccagcgt tacgcgtttg gcatacggaa tgttgtagga aatgccaccg ccttcataac
     8701 tggtaaagcc gctggccatt gagatttcag ccagcagacg cgcatctggc gtgccgtggc
     8761 ggatctgtag cggtttttgc agggtttcgg tgaggcgacg gcaagctgcg acaccatggt
     8821 taactaccgg taaaccgttc agtttagagg ttccggcttc gatggatttt ttaatcccta
     8881 cagccgcttc ttcgtaccga ttcagacggg tatatgcatc gatggtgctt ggcagcagat
     8941 cgcactcttc ttgtagtgtt ttcagcagtt cgatgtgttc gtccatcagc gccacacctg
     9001 cgcgcggttg gctaagggtt ttaccttcct tgtctgcttt cagcagcgca agagagaaac
     9061 gtttatgctc agggatggtt tgttggtatt taacgccatc ttcaaagttt tcaacatcct
     9121 tgccggtttc ccaggttttc agtacctgtt gccgctcggt catgaattcg tcatgggtta
     9181 atttcttatt tcgaagttcc attgattgca gccttaattg ttatagggtt aagcattgaa
     9241 tactggtgcg cgcggcggcc agcggatcga ctctggcgac gtttgccagc agtggtaata
     9301 gcccccggga atcgcggtaa tattcaaatt cggtcggtaa gagaatgcgt ttaccgtcgt
     9361 cgtttaactc gcggtatttc aaccagtgat gcatatcgaa ctgactggca cgcgagagcc
     9421 atccgccgga tccgacgact ttacgcacgg ttgtcaggtc acgtcccatc tgtaaatcga
     9481 cattgccgac gcaggtgcag acctcttttt tggtgcccgc atgtctttcg gtggcataac
     9541 ccacacacag tccggccagg acggtatcga aatatttctc ttctgcattt gcgggtaagt
     9601 agtcgggatg agcgactaga tgacggaggt aatgataaaa agcgtccagc tgtgcgggtt
     9661 gctgagcaaa attcacattg actaactctt tcgcactttc accaaccacc actgccgaga
     9721 cgcgcattcc cagatcgcct tccaccgtcc gttttacgaa gggttccggt acgccgtgta
     9781 aaacggtgtc gggcgagagc gtgttggcac aggcggaata aacatcggtg gttgcgccgc
     9841 ccatgtcgat cagcatgaac tctttccagc tgtgatcgac gttactgatt gccttgacca
     9901 gttcaaagac cgtccatggt gtcggcattg gttcttcgcc tgttttatca acaatgacat
     9961 ccagcccttt ccctttaacg atgcgtttaa ggaatatgtc gcaaatagcc tggcgtgcag
    10021 ctacagggtt cggatggtca agatcgggca gaacgttgtc gacaatggtc aggtctttat
    10081 gcccgaggat ctcctgcacc tcgtcctgaa tatcccggtt tccggcataa ataatggcgc
    10141 agtcgagttt ggattctgcc agcacacggg cgttattcag tccgtagctt tcttctccgc
    10201 cgtcggtgcc gccggtgaac aagagaatat cgggctgcgt ttcttccagt gcctggatat
    10261 cacggcggtt cagcttgtag gcgtaatatt gcgcaatttt agcacccgca gagtgagcgg
    10321 ttactttcgc ggtttccagg gtgatagaag gcaccaatcc cattgcggca accgccagcc
    10381 cacctttggc agaggaggaa tacttcaatg caacctcacc gctgtttaac agggggaggg
    10441 cattatctac gtttaacacc tggttcaggc tggagaaaaa ccccttcgcc agatgatggg
    10501 tggtggttgg ggtcagtacg tggttaacca acgttaacgc gtccccctcc cgggtaaaga
    10561 gggctgcttt ggtccacgtc gatccaatat cgacagagac gatttgcatc agatagcctc
    10621 ttcaagaata cgagtatcaa cgtcatgacg ttggttgata tcgtgggcca tcagctggca
    10681 aacatcttct aagtcatgac ttggggcaaa gacgcgatca aatcccatct ctttgaattt
    10741 ggtttccacg tcagcgaagt catgttttcc gaccaccagg ttaccgccca catagagcag
    10801 gatgtccccc agtccacgct cgatgcagcg ttcgcgcatg cccagacagt cgatatcgcc
    10861 atgcccatag atagaggaga cgacaatcgc gtcagcgcca gtttcaatgg cagcatcaat
    10921 atattcgtcc tggctgacca ttacgcccag attaatgact ctgaagtcgt ggttgctaaa
    10981 aacgcggtcc agaactttat tgcctactgc atggcagtcc gcgccaataa cgccaatcac
    11041 aagtgttgct ttcttcatgg gtaatccttt gtaagacaga gagtaaaata agttacacac
    11101 gtcgtattta tggccgttca taacggcaca ggtttaatat cacaggagtg tctttatctg
    11161 accgtaatgt tgcaattgat ctattttcaa aatgtctttc gggaaatgat tgtttgaagt
    11221 gtgtttttaa atttccatgt taattcagtg tgttatgtca actaatgtca tgagtgttaa
    11281 atttttaaaa ttaacttgat ttaatgtatt gagataattg tgatctactt ctaatttgca
    11341 tttttgtgac gtggttttag ccatgagtaa atgcatttca taagatttaa gtcgaatttc
    11401 actgcaattc atctctgctt aatgtaaaaa taatgttgaa aataacaaat gg
//
LOCUS       DAZFKY010000151        11415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFKY010000151 DAZFKY010000000
VERSION     DAZFKY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            254..553
                     /gene="yoaC"
                     /locus_tag="ACHKCL_003609"
     CDS             254..553
                     /gene="yoaC"
                     /locus_tag="ACHKCL_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5764494.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKCL_003610"
     CDS             complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKCL_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5764495.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            926..2287
                     /gene="pabB"
                     /locus_tag="ACHKCL_003611"
     CDS             926..2287
                     /gene="pabB"
                     /locus_tag="ACHKCL_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5764496.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKCL_003612"
     CDS             2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKCL_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5764497.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKCL_003613"
     CDS             3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKCL_003613"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5764498.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKCL_003614"
     CDS             4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKCL_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5764499.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKCL_003615"
     CDS             complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKCL_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5764500.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKCL_003616"
     CDS             complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKCL_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5764501.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8169..9140
                     /gene="manX"
                     /locus_tag="ACHKCL_003617"
     CDS             8169..9140
                     /gene="manX"
                     /locus_tag="ACHKCL_003617"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5764502.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9203..10003
                     /gene="manY"
                     /locus_tag="ACHKCL_003618"
     CDS             9203..10003
                     /gene="manY"
                     /locus_tag="ACHKCL_003618"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5764503.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKCL_003619"
     CDS             10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKCL_003619"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5764504.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKCL_003620"
     CDS             10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKCL_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5764505.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2800 a         2877 c         2867 g         2871 t
ORIGIN      
        1 catgccgttg tgttcgccct ttgattagca actctggttt tctttcttag ctgaacaatc
       61 tggttatagc gtgcggactg accgttaatt ttctccatct tactggataa cacaccatga
      121 gctgctacct ttaaaacacc tgaatatcaa caggttaact cgtgccatat cgttataatc
      181 cctttgatat tagatgcaaa ttaaggtcat atatagcctt atcgataaca tggttaattt
      241 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
      301 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata
      361 tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga
      421 aggctggaac cctgggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagacagg
      481 tgaacgctct gtgattaaaa atcctgagta cttttcaaca tatatgcagg aagaactaaa
      541 agcactggtc tgagttaaat ttatatcagc ataaatgggt caggacgctt ttaatcacat
      601 ataaaaagcg tcctttttct cacaatcaaa taatcatcag cttctataac gaaatcgata
      661 cggattagtg tgttactcgt cttcatcctc aaaacgcgcc acgattcgct cgccggagtg
      721 gttggcacgc agctcttctg ccaccagcgc gattgcctgg ccgctgctca tcccttgtgc
      781 catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa
      841 acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg
      901 cacatagcca gtagagtcag gactgatgaa gacgttatct cccgctgtga ttactttacc
      961 ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc cgtgggcgat
     1021 gcttttacac tccggctatg ccgatcatcc gtatagccgc tttgatattg tggtcgccga
     1081 tccgatttgc actttaacca ctttaggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
     1141 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat
     1201 tcgcccaacg cataacgaag atttgccatt tcagggcggc gcactggggt tgtttggcta
     1261 cgatctgggc cgccgttttg agtcactgcc agaaattgcg gaacaagata tcgttctgcc
     1321 ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc gtcatacagt
     1381 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
     1441 ctcaccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgagca
     1501 gtacggcgaa aaatttcgcc gggtacagga atatctgcac agcggtgatt gctatcaggt
     1561 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
     1621 gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac agggtgcaat
     1681 tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc agacccgccc
     1741 gattaaaggc acgctaccac gcctgcccgc tcctcaggaa gatagcaaac aagcagaaaa
     1801 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
     1861 tgatatcggt cgtgttgccg tagcaggttc ggtaaaagta ccagagctgt tcgtggtgga
     1921 acccttccct gccgtgcatc atctggtcag caccataacg gcgcaactac cagaacagtt
     1981 acacgccagc gatctgctgc gcgcagcttt tcctggtggc tcaataaccg gggctccgaa
     2041 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg
     2101 cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta tccgcacgct
     2161 gactgccatt aacggacaaa tatactgctc tgcgggaggt ggaattgtcg ccgatagcca
     2221 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
     2281 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatcgc gctttcaact
     2341 tttgcgcccg caaattaacc gggaaaccct aaatcatcgt caggctgccg tgttaatccc
     2401 catcgtccgt cgaccgcaac cggggttgtt gctgactcag cgttcaattc atctgcgtaa
     2461 acacgctgga caagtggcat tccctggagg tgcagtcgat gacacggacg catcagttat
     2521 cgccgccgcg ctgcgcgaag ctgaagaaga ggttgctata ccgccttccg ccgttgaagt
     2581 tatcggcgtg ctgccgcccg tcgatagcgt aactggctac caggtaaccc cagtggtcgg
     2641 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtttccg ctgtgtttga
     2701 aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata tctaccgccg
     2761 cggcgattca catcgggtat ggctatcctg gtacgaacag tattttgtat ggggaatgac
     2821 cgcaggcata attcgtgagc tggcgctgca aattggtgtg aaaccctgac tatacttatc
     2881 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
     2941 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
     3001 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
     3061 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
     3121 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
     3181 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
     3241 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
     3301 tcccggtttt attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca
     3361 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
     3421 tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta
     3481 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atactgccaa
     3541 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga
     3601 aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
     3661 agagatcgac gagtatttcg cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
     3721 gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct
     3781 gcgccggatg ctggtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg
     3841 ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc gtgtggtaac
     3901 tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg ctggcttact atgaccactt
     3961 tattgaatcg gtcagcccgg acatctatac ccgttacttt atggcagcgg gcgcgattgg
     4021 tgcattgtat aaaatgaacg cctctatttc cggtgcggaa gttggttgcc agggcgaagt
     4081 gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga
     4141 acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa cctgcgaccc
     4201 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc
     4261 gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct cgctggataa
     4321 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc
     4381 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca
     4441 acggatgggc gaatttatcc ccgctttctc gtctgctgta atattcccca ctacacttcc
     4501 actgttgcgt caggcgtttg tcgccatacg cttacagagt ggcccgcatg caaaaagcac
     4561 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
     4621 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
     4681 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
     4741 aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
     4801 cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
     4861 gcacacttta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
     4921 ctgaacttcc tgcaccgcaa ccacttttac gcctgacgac cgaccgtgtc ctgattaaag
     4981 gcagtccggt tttgattcaa tggacgcctg cagcgagcag tagcaatgct ggggtcatgg
     5041 agatgattaa catcgactta ctgacggcaa tgctgcttga gccacaactg ccgcaaatca
     5101 gtagcgccag cctgacggtg gacaaacggc atttgctcta tggtaatggg ctggtagatt
     5161 cccttccaca acctgaagac aatgaaaact atcaggtttc ttcgcaacgc ttttctttta
     5221 ccattaacgt taatggtccg ggggctccgg cgctggcatg gcactatctt ccaacacaat
     5281 taccgctggc ggtactgcta agtttactgg tgggctacat cgcctggctg gcgaccgctt
     5341 accgaatgag cttttcccgc gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt
     5401 tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag attttgctgc
     5461 gctggaacaa tccgcgtcag ggctggattt caccggatgt gtttattcct atcgcggaag
     5521 aacatcattt aattgtgcca ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc
     5581 atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc
     5641 gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc
     5701 aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc
     5761 gcgagctaca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaacagtt
     5821 cattttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg
     5881 cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc gcgctggggc
     5941 aaagactgaa tattgaactg gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt
     6001 tgcgccgtca tggcgtgcat attttgcaag ggtatttgta cgcacagccg atgccgctac
     6061 gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata
     6121 tcacgcccat tatgccgtta cgttaacgct tactcatctt catcgtgcgc cggttgctct
     6181 ttaacaatgc gaaccagatc aacacgataa tcattggctt caatgatggt gatatgcagt
     6241 ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg
     6301 cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc
     6361 aaggcatgca aatctgtacc gccttttacc agccagccgt caccatcagt aatgatttcc
     6421 ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg
     6481 accaagcctt gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcacga
     6541 cgcagcacgc ccagcaggtt gatcggatcg agggtttcag ggacgataat cgccggagac
     6601 gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca
     6661 cgtacgattc cgatgatttc atccagttca ccgcgacata ccgggaacag gctgtgcggt
     6721 gaggagagca gctgctcgcg gatttcatcg accccgagat tagcgtcaac ccagcttatt
     6781 tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta
     6841 atcatgtaac gttcttcttc ggcaaatgca ccttccggga tcggcatcgg catcgggtta
     6901 tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg
     6961 gcagtacgcg ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg acgcgcaatc
     7021 tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga
     7081 atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag gaagctcaga
     7141 cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt ggatgccagc
     7201 aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg gttaaccatc
     7261 cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac aatctgtgtg
     7321 acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc
     7381 agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc gaacaacata
     7441 atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg
     7501 accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataacccc
     7561 agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag
     7621 accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa
     7681 attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg
     7741 ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat agagtgccag
     7801 aaaggcaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg
     7861 ctactgccct atactccatg attgttaaac gggagttaaa catatcagag acgcctctga
     7921 tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa
     7981 atttctgcta atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata
     8041 tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg
     8101 attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga
     8161 ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc
     8221 ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc
     8281 caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca
     8341 ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc aatgctgcca
     8401 gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc
     8461 tcgtggaaac gttaatggct cgtgatgatg acccaagctt tgatgaactg gttgcgctgg
     8521 cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccggtt gaaaaagccg
     8581 cgccagcacc cgctgccgca gcacccaaag cggctccaac tccggcaaaa ccaatggggc
     8641 caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg
     8701 tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag
     8761 tggctgcgga tactgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag
     8821 cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg
     8881 aacgtgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg
     8941 tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaagtga
     9001 ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caaaaaactg aatgcacgcg
     9061 gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga
     9121 tcagcaaaat cgataagtaa cgtattgtgt tgattatcag tcagttttca cactttagtc
     9181 atacgtaaac aggagaagta caatggagat taccactctt caaattgtgc tggtatttat
     9241 cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct
     9301 aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta ttattatcgg
     9361 tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga
     9421 cgccgctctg gcatctatca tttctaccat tctggttatc gcaggtcatc agagcattgg
     9481 tgcaggtatt gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt
     9541 tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata acggcaacct
     9601 gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggctat
     9661 tccggccgtc atcgttgcgc tgtctgtagg taccagcgaa gtacagaaca tgctgaatgc
     9721 gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg tggtggttgg
     9781 ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg
     9841 cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt
     9901 tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc
     9961 tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt
    10021 tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt
    10081 cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt
    10141 ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg aagctcgtaa
    10201 acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg ctgcgccgat
    10261 tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgacgacgg
    10321 tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat
    10381 cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg cgatgagcgg
    10441 cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg
    10501 ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg
    10561 tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc
    10621 attggttaac aagtggacac atgtcaacat cccgctggtt gtctctcgca ttactgacca
    10681 gacgggcaaa gaacacgtta ctactgtcca gactattctg gaccagttaa tgccaggcct
    10741 ggtaccactg ctgctgacct ttgcttgtat gtggctactg cgcaaaaaag ttaacccgct
    10801 gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg
    10861 actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt
    10921 aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg ccttcgcgat
    10981 ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa ttcctttgct
    11041 ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc ttatctataa
    11101 caacgtcacg aatcatggtg cgttaataac gacatggtta ttaagcgcac tggctctgat
    11161 gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt
    11221 cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg
    11281 cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga
    11341 tctggaaaga atttataaac ttctcgtttc aactcaatga gttattaatt tagccaaagc
    11401 tatgtttagt gtatt
//
LOCUS       DAZFKY010000152        11408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFKY010000152 DAZFKY010000000
VERSION     DAZFKY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..417
                     /locus_tag="ACHKCL_003621"
     CDS             <1..417
                     /locus_tag="ACHKCL_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5764506.1"
                     /translation="LQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLND
                     DQRRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRAL
                     AAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            432..1289
                     /gene="menB"
                     /locus_tag="ACHKCL_003622"
     CDS             432..1289
                     /gene="menB"
                     /locus_tag="ACHKCL_003622"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5764507.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1289..2251
                     /gene="menC"
                     /locus_tag="ACHKCL_003623"
     CDS             1289..2251
                     /gene="menC"
                     /locus_tag="ACHKCL_003623"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5764508.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2248..3603
                     /gene="menE"
                     /locus_tag="ACHKCL_003624"
     CDS             2248..3603
                     /gene="menE"
                     /locus_tag="ACHKCL_003624"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5764509.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3713..3979
                     /gene="pmrD"
                     /locus_tag="ACHKCL_003625"
     CDS             3713..3979
                     /gene="pmrD"
                     /locus_tag="ACHKCL_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5764510.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(3973..4359)
                     /gene="arnF"
                     /locus_tag="ACHKCL_003626"
     CDS             complement(3973..4359)
                     /gene="arnF"
                     /locus_tag="ACHKCL_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5764511.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4359..4694)
                     /gene="arnE"
                     /locus_tag="ACHKCL_003627"
     CDS             complement(4359..4694)
                     /gene="arnE"
                     /locus_tag="ACHKCL_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5764512.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4691..6343)
                     /gene="arnT"
                     /locus_tag="ACHKCL_003628"
     CDS             complement(4691..6343)
                     /gene="arnT"
                     /locus_tag="ACHKCL_003628"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5764513.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6343..7233)
                     /gene="arnD"
                     /locus_tag="ACHKCL_003629"
     CDS             complement(6343..7233)
                     /gene="arnD"
                     /locus_tag="ACHKCL_003629"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5764514.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7230..9212)
                     /gene="arnA"
                     /locus_tag="ACHKCL_003630"
     CDS             complement(7230..9212)
                     /gene="arnA"
                     /locus_tag="ACHKCL_003630"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5764515.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9212..10180)
                     /gene="arnC"
                     /locus_tag="ACHKCL_003631"
     CDS             complement(9212..10180)
                     /gene="arnC"
                     /locus_tag="ACHKCL_003631"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5764516.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10184..11323)
                     /gene="arnB"
                     /locus_tag="ACHKCL_003632"
     CDS             complement(10184..11323)
                     /gene="arnB"
                     /locus_tag="ACHKCL_003632"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5764517.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2782 a         2950 c         3050 g         2626 t
ORIGIN      
        1 ctgcaaaatg ctgaacaacg cgcggaacgt cagcgttccg atcgccaatg ggcgcagcgt
       61 tttcgttccg aaccattaac cgcggtattt gccgactggt atcaacagcc tgtttttgcc
      121 tcgctcaatg acgatcaacg ccgggagctg gtggtgctgc gcagcaacaa taatggcgca
      181 acccttgccg ccatgctgga ggcgacttct ctcgccgtcc agcctgattt acgtgctaac
      241 cttagcgccc gcacatttgc gttttattat ttatgtggtg aacgtgacag caaattccgc
      301 gccctggcgg cggaactggc tgccgactgc catgtcattc ctcgcgccgg acataacgcg
      361 catcgggaaa atcccgctgg cgtaatcgca agtctggcgc agatcttgcg tttctgacta
      421 aaggacacaa tatgatttat cctgatgaag caatgcttta cgcaccggtt gaatggcacg
      481 actgctccga aggctttgag gacattcgtt atgaaaaatc caccgacggt atcgcaaaaa
      541 tcaccattaa tcgtccgcag gtgcgcaatg ccttccgtcc tctgacggta aaagagatga
      601 tccaggcact ggcagatgcg cgttatgacg ataacgtcgg cgtgatcatt ctgactggcg
      661 caggcgataa agcgttctgc tccggtggtg accagaaagt gcgtggtgat tacggcggct
      721 ataaagatga ttccggcgta catcacctga acgtgctgga cttccagcgt cagatccgta
      781 cctgtccgaa accggttgtc gcgatggtgg ctggctattc catcggcggt ggtcacgttc
      841 tgcacatgat gtgcgacctg actatcgcgg cagataatgc catcttcggt cagactggcc
      901 cgaaagtcgg ttccttcgac ggcggctggg gcgcttccta catggctcgt atcgtcgggc
      961 agaaaaaagc gcgtgaaatc tggttcctgt gccgtcagta cgacgcaaaa caggcgctgg
     1021 atatgggcct tgtcaacacc gtggtaccgc tggcggatct ggaaaaagaa accgttcgct
     1081 ggtgccgtga aatgctgcaa aacagtccga tggcgctgcg ctgcctgaaa gctgcactga
     1141 acgccgactg tgacggtcag gcaggtctgc aggagctggc gggcaacgcc accatgctgt
     1201 tctacatgac ggaagaaggt caggaaggtc gcaacgcctt caaccagaaa cgtcagcctg
     1261 acttcagcaa attcaaacgg aatccgtaat gcgtagcgcg caggtatacc gctggcagat
     1321 ccccatggac gcgggggtgg ttctgcgcga caggcggtta aaaacccgcg acgggctgta
     1381 cgtttgcctg cgtgagggcg agcgcgaagg gtggggggag atctccccac tgccgggctt
     1441 cagtcaggaa acctgggaag aggcgcaaag tgtgctgctt gcctgggtaa ataactggct
     1501 ggcaggcgat tgcgaattac cgcagatgcc ttccgttgcc tttggcgtaa gttgtgcatt
     1561 ggcggagctg gcagatacgc tcccgcaggc ggccaattac cgtacggcac cactgtgtaa
     1621 tggcgatccg gacgatctga tcctcaaact tgcagatatg ccaggcgaga aagtggcgaa
     1681 ggtcaaagtg ggattgtacg aagcggtgcg cgacggcatg gtggtgaatc tgttgctgga
     1741 ggcaattccg gatctgcatt tgcgtcttga cgcaaatcgc gcctggacac cgctgaaagg
     1801 tcagcagttt gccaaatacg ttaacccgga ttatcgccac cgcatcgcgt ttctcgaaga
     1861 gccgtgcaaa acccgcgatg attcgcgagc gtttgcccgt gaaaccggca ttgccattgc
     1921 ctgggatgaa agcttgcgcg agccggattt tgcctttgtg gctgaagagg gcgtgcgcgc
     1981 ggtagttatc aaacccacgc tcacgggcag tctggagaaa gttcgcgagc aggtacaggc
     2041 ggcgcacgcg ctggggctga cggcagtgat cagttcttcc attgaatcga gcttaggctt
     2101 aacgcaactg gcgcggattg ctgcctggtt aacgccggac accattccag ggctggacac
     2161 gctggatcta atgcaggcgc agcaggtacg tcgctggccg ggtagcacgc tgcctgtcgt
     2221 ggaagttgat gcactggagc ggttgttatg atcttctctg actggccgtg gcgtcactgg
     2281 cggcaagtgc ggggagaagc catcgcctta cgtcttaatg acgagcaact caactggcgc
     2341 gagctttgtg ctcgcgtcga tgaattagcc tccggatttg cggtgcaggg tgtggttgag
     2401 gacagcggcg tgatgttgcg ggcgtggaat acgccgcaaa cgctgctcgc ttggctggcg
     2461 ttactgcaat gcggggcgcg ggtgttgccc gtgaaccctc agctgccgca accgttgctt
     2521 gaagaattgc tgcccaatct gacgctgcaa tttgcgctgg tgccggatgg ggaaaacacg
     2581 tttccggcat taacgtcgct gcacattcag cgggttgaag gcgcacatgt cgctacgtgg
     2641 cagccgacgc gtctgtgctc aatgacgttg acctcgggtt ctaccgggtt gccgaaagcc
     2701 gctgtccata cttatcaggc ccatcttgcc agtgccgaag gggtgttatc gctgattccg
     2761 tttggcgatc acgatgactg gttgctctct ttaccgctgt ttcacgtctc cggtcaggga
     2821 attatgtggc gctggttata cgctggtgcg cggatgacgg tgcgtgataa acagccattg
     2881 gagcaaatgc tggcaggctg tactcacgct tcactggtgc caacacaact ctggcgtttg
     2941 ctggttaacc gcagttccgt ttccctgaaa gcggtgttac ttggcggcgc ggctatcccg
     3001 gtcgagttga cggaacaggc gcgcgagcag gggattcgtt gcttttgcgg ctatggtctg
     3061 accgagtttg cctccacggt gtgtgcgaaa gaagccgacg gcctggcaga cgttggttcg
     3121 ccgctgccgg gtcgggaagt gaaaatcgtt aataatgaag tgtggctgcg ggctgccagt
     3181 atggcagaag gttactggcg taacgggcaa ctggtttcac tggttaatga cgaaggctgg
     3241 tacgctacgc gcgatcgcgg tgagatgcat aatggcaagc tgaccattgt cggacgttta
     3301 gacaatctat tcttcagtgg cggagagggt attcagacgg aagaagtcga gcgcgtaatt
     3361 gctgcacatc ctgcggtttt gcaggtgttt atcgtccccg ttgccgacaa ggagtttggt
     3421 catcgaccgg tggcggtgat ggagtatgac cacgagagcg ttgatcttag tgaatgggtg
     3481 aaagataagc tggcccgttt tcagcaaccg gtacgttggc tgactttgcc gccggagctg
     3541 aaaaacggcg gtattaaaat ttcacgtcag gcgctaaaag agtgggtgca acgtcagcaa
     3601 taattaaccc tccgtttcac ttctcagttc tgccattcca ttgccgttga taaagagttt
     3661 atctgttaat ctgtaaattg atatgaaaac tcttagcaaa caggataata caatggaatg
     3721 gctggtcaaa aaatcgtgct gcaataaaca agataacaga catgtactta tgctctgcga
     3781 tgctggcggc gcgataaaaa tgatcgccga agtgaagagt gacttcgccg tgaaagtggg
     3841 ggatttactc tcgcctttgc agaatgcgct ttattgtatt aatcgtgaaa agctgcatac
     3901 ggtgaaagtc ctctctgcaa gctgttatag ccccgatgag tgggaacggc agtgtaaagt
     3961 ggcagggaaa actcagtaac gttgttttgt cgtgggcagg aaaatcagca tcaacccgct
     4021 cataatacag gctactccca gtagtgcttt cagcgaaaaa gtcccttccc agccgggaag
     4081 taccattgaa gcaatccata ccagcacata actcatactg agcaaggcat aggctttgct
     4141 tagcgcaagt ttatgtagtg ttttatacca acaaaaaacg gaaagcagat aacccagcag
     4201 cccgagtaac agtatccggg catccagccc aaaagctaac agcgccgcga taaaatccca
     4261 taagtgcgtc atcggcggca gatgactcgc cgcaaaaccc aggcttaatt gcgcaactga
     4321 ggcaataatg acgctgaata atccccatat caggcccatt acaccgtact cccgaggatc
     4381 acaatgccac caataatgaa cgccacccca caccagtgac gcggcgatac cggttcgtgc
     4441 cacagtttta ctgcagccag cgtcacccag acaaaattca gacttaacat cgggtaggca
     4501 atacctaccg gtacgttctg caagaccagc agccagagca ccatggcaag accaagacaa
     4561 gccagcgcca gtcccagcca cagcacgata tgtttgcgcc gtttgcttat cgccacaaag
     4621 caggttgcct gtttctgaca caactgcccg gcaacgctaa gcaagctggc aaagactaat
     4681 gttagccaga tcatttggga cgatactgaa tcagcaccag acgctcctga cgatcgatgg
     4741 catctgcggg cggaatggcg agactgttga tatcttcatc gcggtcaacc gaaagcacga
     4801 gtgtaataat cccctcctga cgatgttgat taagccagtt tgcgaactca tcaccgctga
     4861 caaatctccc tttcgcatcc ggataattaa ggccgtattt caactcacct gtctggcgat
     4921 acatgatgat gtcatcgcgt tgcagactcc atgccagacc tgcggcaaca ccgacgctat
     4981 cagtaagaat atagcggctt ggctgaagtg attcttgtgt catctcgaca aaaaattgcg
     5041 gatgttttcc ttccatgact ctgtcaggaa ttgaaaatcc taccagcaac gccagcccca
     5101 gcgggcaaag cgcggcaaaa gaccaggtct tttcgacgtt tgttaaggtg taccagccga
     5161 aaaatgccca tagcgaaaaa atcgaccagg cacaaaagac tttatagctc tcgaaggttt
     5221 gccacaccgg cgtgttcatt ggtccccagg gggagaccac aaatgtggca ataatgccag
     5281 tgacgccaaa agcgatgtta atccagccat taatccgcag cgccagagga ttattttttg
     5341 ctgccagcaa agcgtaatgc gccatcagca ttgccaaagg tgcaaagcag gaaagaatat
     5401 aggtgggcaa tttaccctta gcaacggaga aaaacagcag cggcattatc gtccagctca
     5461 acaaatagac ggttgcggaa tgcttgcggt ttttccagcc tgtgtatagt gcaccgggga
     5521 gtaatcccag ccacggcaga ctaccggcaa tgatgaccgg cacgtagtac cagaacggag
     5581 ctctatgttg ggcatcatcc agtgcaaagc gttgaatatg ctcaacccag aaaaaatagt
     5641 gccagaagtc aggctcccgc tgcgctatcg tcagtcccca ggggagaacc gtcagtacgc
     5701 aactgatgac cgccagccag ccgtaaataa agagatcttt ccagcgtttt tgcgtcgcta
     5761 cccatggcag cacgcttaat accggcacgg caagggcgag aaaacctttg gtcatcaccc
     5821 ccatgccgca ggtgattccc agcagtaaaa atcctgcgct tttgcctttc cacgtctgtg
     5881 cctgcattgc cagccagaag ctgcacattc ccgccaccag ccagaaggca ataaacggat
     5941 cgagcacggc ataagtgccg atggcataga caataaacaa tgagagataa attactgtgg
     6001 cgagtagagc cagacgttta tcgcgccata agcgcagagt aaaccaggtc accagcgcgg
     6061 cagttaacag ggtcgcaaag ataacgcctg cccgcacacc aaagttattc gcgccaaata
     6121 gccattgccc aatgctgtta atccagtatc cggcaatggg tttttcaaaa taacgtagcc
     6181 ctaacagatg gggcacaatc cagtcgccgg atgccagcat ttctcgactg atttccgcat
     6241 aacgcgtttc atcgggttgc catagcagac gcgtgctgat cggtaacagg taatagcagg
     6301 caataaacgc gaagaggccg ataaggtaac gtaccgattt catcagcgac tacccgcaat
     6361 ttgttggcaa cccagccagc cttcacgtcc ggcaatattt ccgcgaacaa cttgtccgag
     6421 cggcaacgtc tctgataaca gttcgcttaa agggcaaaat gtcacgcctt cctgagctgc
     6481 gcgtttgagg agatccacaa aattatgctg ataagcgcag ccttcaactt ctgcatgaat
     6541 ggtataaacc ggcgtgcctt tatctcgcag gatgcggttg agtaaccaac cgttaaaatc
     6601 ttctgctttc acatcccgac caatcacttc atcccaggtg ggtaaggtca ccggaatttg
     6661 cgcagtgcca gggtttcctg attcgagcag tggacggaac ggcatggccc cacgacaatc
     6721 gctgttgtag cgcaaatgga acgcttcttt tgcttcgatc acctgctggt cggcacgcca
     6781 gcccgcagcg gcagaacagg ttaccggttg accgataatc tcttccagag tgcgaaggcc
     6841 gcgtgcaata tcgtcgatca ttgtttgccg atcccagtta ccgctacggg cttgccaggc
     6901 atggtgatcc caggcgtgca ggccgacttc gtgatattta gccgcttcac gaatgatatc
     6961 ggcattggca tgaccaatct ctttacctgg ccaggccgta cctgccagta aaatatccca
     7021 gccataaagc gatgccgcgt ttgagcgcag catcttccac aaaaactgtg gcttcaccag
     7081 tcgccagaga tggcggccca tattgtccgg gccgacgctg aaaaaaatgc tggcctgaat
     7141 attatgctta ctcaagattt ccagcagacg cggcacgcct tcacgggtgc cacgaaaggt
     7201 atcgacatca atgcgtaagc ctactttggt catgatggtt tatccgtaag atcaacggtg
     7261 cgcaggaaga aatccagcgt ttcgtcgatg gtttcctgca tatcaatttt cggctcccag
     7321 tccaggcagt ggtgggcatt gcggatgctc ggtttacgat gctctacgtc ctgatatcct
     7381 ttgccgtagt agctgctact ttcgacaacg cgaaagcccg caaacggtgg gaaatgatgg
     7441 cgcagcggat gtttttcgaa gctcgccagc agcatctcgc ccagttcctc aatgctcgct
     7501 tcgttctcag gattgccaat gttgataatt tcgccgtcgc agcgatttcc cgcattttcg
     7561 ataatgcggt ataacgcctc gataccatcg cgaatatcag taaagcagcg tttttgtttt
     7621 ccgccatcaa tcagcttaat cggtgaacct tctaccagat tgagaatgag ttgcgtaatc
     7681 gcgcgggagc tgccgattcg tgccgcatta aggttatcca gtcgtggtcc catccagtta
     7741 aacgggcgga agagggtgaa ctgtaaaccc tctttttcgc cataggccca gatcacccga
     7801 tcaagtaatt gttttgatac cgaataaatc cagcgtggtt tattcaccgg gccgacgatt
     7861 aaattagaat ggtcctcatc gaagtattta tcgctacaca tcccataaac ttctgaagtt
     7921 gacgggaaga tgattcgctt acggtacttc acgcagtagc ggataatgcg cagattctct
     7981 tcaaaatcga gttcaaatac gcgcagcggg ttgcgggtat attcaatcgg cgtggctatc
     8041 gccaccagcg gcaagacgac atcacatttt ttgacgtgat actcaatcca ttcggaatga
     8101 atactgatat cgccttcgac aaagtgaaaa tgcggatgat tcagaaaacg gcttatcgca
     8161 tcgctgccaa tatccagacc gtaaacttca taatgatctt cgcgcagcag gcgttctgtc
     8221 agatggttgc caataaagcc attcaccccg aggatgagta cccgggtacg gcgtcgggcg
     8281 gtgcaggcag gctggctatt caagcgtgaa ccttgcacca ggcccagcgt ctgcgctaat
     8341 tgcgagccct gcatagtaat gccgtcgccc gcttgtccgg tgacgatttc cagcgcgcca
     8401 tcgccacagg caatcagcag tggcgcaaca gaaatcacgc tccccggctg tgctttgctg
     8461 gcatgaggat gaacacgcga cgaccagacg gtgaatttct gattgccaac atagctgaag
     8521 gcacccggcc acggatcggc aacggcacgt accatgttgt gcagtacgga tgccggctta
     8581 tgccattcga ggaaactgtc atccggcgtt ctgcgaccaa aacaggtggc ttcgttttcg
     8641 cgctgggcga tttccagaat attaccgtgt ttaatggcgg gtaatgtctg ttccagtagc
     8701 tggcgcgcgg catggcacaa tttatgatgc agcgtaatag cgatatcgtc tggcgcaatg
     8761 gcaacgcgca gttgcgccac aatggccccg gcatcggcac gttttaccat tcggtgcaat
     8821 gtaacgccag tttctgtttc accgttcacc agcacccagt tcagcggcgc gcgaccacga
     8881 tattttggta acagcgaacc gtgcagatta aatgcacctg cgggagcgag ctgcaaaatc
     8941 tcgtcgcaaa taagatggcg ataataaaaa gagaaaatca cctctggtga gagttgggca
     9001 atgcgttcca cccacagcgg atgattaacg ttatccggcg cataaaccgg aatgcctctt
     9061 tccgccgcca gacgagccac cgaaccataa aaggcttttt caccgggatt atcggtatgg
     9121 gtaaaaatgg cgctaatttc gtaaccggca gccagcaggg cttcaatacc gaggcatccc
     9181 atatcgtggt aggcaaaaac gacggttttc attattcatt ttccttgctg gatggacgga
     9241 taacttgctg aacaaaatag cgggggcggg cgcggacatc ggtgtagatc ctgccgatat
     9301 attcaccgag taatcccatg ccgataaact gagcgccaat aaaagtaaac agcacggcaa
     9361 atagcataaa gacgccttct gccgcccatt gtggtccgaa ggtcaggcgt aaaatcacca
     9421 gcagcaccgc aatgctaaaa cctccaatcg caataatgct gccgagcaga ctcagcatac
     9481 gtagcggcgt tgtggtaagg caggtcacca ggtcgtacat caaattaatc aggcgcataa
     9541 agctgtattt ggattcacca tactcacgct cggcatgatg cactggaatt tcaatggcac
     9601 ggcgggcgaa gatattcgcc agaatcggga taaaggtgct gcgttcatgg cagtgcaaca
     9661 tcgcatcgac aatatgacgg cgataggcgc gcagcataca accgtagtca cccatcgctt
     9721 tgccggtggt gcgctgaata agccggttaa tcatcttcga agctgtttta cgaaaccagc
     9781 tgtcctggcg gttctggcgt acagtcccta ccacgtcgta accttcatcg gcttttgcca
     9841 ccaggcgggg gatttcttct ggcggattct ggagatcggc atcaagggta ataattaagt
     9901 cgccagtaac gtgactgaat cccgccataa tcgctgaatg ttgcccgtaa ttgcggttaa
     9961 gcaaaataga tacaatatgg ctgttctccg cttgtgaggc ttcgaccagt atatgcgcgg
    10021 aattatcgct actgccgtca tcaatcagca ggatctcata ctctttcccc aacgattcac
    10081 aggctgtggt ggtgcgcctg attaattccg gtaagctttc ctgctcgtta taaacgggaa
    10141 taaccaccga gactttctta acagggtgga tttcaaacat ggcttattgt cctgcgagtt
    10201 gctgaagggc tgtgatgaca cggtcggcat cggcggtagt catatccggg aacagcggca
    10261 aagaacagat gcgttcgcta ttccattcgg tattcggtaa cgacagcgag ggaaaacgct
    10321 cgcgataata tttttgtgtg tgagcggcgc ggaaatgtaa cccggtacca atacctcttt
    10381 cttttaacgc ttccatcaac gcatcgcgac tgataccaca acgttgttca tcgacacgaa
    10441 taataaacag atgccaggcg tgaacgtgcg gccaggcggg aaggcttaat ggctgaaagg
    10501 ggagagctgc cagtgcttgc tgatattgct gggcaatttc gcgccgacgg gtgttgaggt
    10561 gctctaattt ggctaactgt gtcagggcaa tcgcggcgtt aatatcggtc agattgtact
    10621 tatagcccgg cgttaagact tcagcctgcg gtgcacggcc ccaggtttgt ctgtcatagg
    10681 catcgacacc caggccgtga aatttcagca tccgtagctg gcgggcaata ttttcattat
    10741 cagtaacaat caggcctcct tccgcacagg taatattttt aatggcatga aatgaaaaaa
    10801 tagcggtacc ttttgcgcca atatgtcgcc ctttgtaata cgtacctacg gcatgggcag
    10861 catcttcgat aactgcgatg ccgtaacgtt cgccaatggc gcgaatggcg ttaatatctg
    10921 ctggcgcacc ggcataatgc accggaatga tggctttagt gcgtggcgta atggctgatt
    10981 cgatcgcttc aggcgtgacc atcagcgtat cgcgatcgac atccaccatt accggcgttg
    11041 caccctgcaa ggaaatcata ttgagggttg aaacccaggt cagggaaggc gtaatcactt
    11101 catcgccctt gccaatttcc aacgccatta gcgtgatatg cattccggcg gtggctgaac
    11161 tgaccgcgat ggcatgctga tttcccgtca actggcaaaa ggcttgttca agcgcgtgat
    11221 tcttcggacc ggttgtgatc caaccggatt cgagaacctc tttaactgca gcgagttcct
    11281 ccacgcccat tgctggtctc gaaaaaggca aaaattctga cattgctttt ccttccgcca
    11341 ttgaatacct gtccacttat atttgctata aagagtgttg tgtatatttt gccatttaga
    11401 gcgaaatt
//
LOCUS       DAZFKY010000153        11390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFKY010000153 DAZFKY010000000
VERSION     DAZFKY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(237..2066)
                     /gene="glmS"
                     /locus_tag="ACHKCL_003633"
     CDS             complement(237..2066)
                     /gene="glmS"
                     /locus_tag="ACHKCL_003633"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5764518.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2228..3598)
                     /gene="glmU"
                     /locus_tag="ACHKCL_003634"
     CDS             complement(2228..3598)
                     /gene="glmU"
                     /locus_tag="ACHKCL_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5764519.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3950..4369)
                     /gene="atpC"
                     /locus_tag="ACHKCL_003635"
     CDS             complement(3950..4369)
                     /gene="atpC"
                     /locus_tag="ACHKCL_003635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5764520.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQLGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4390..5772)
                     /gene="atpD"
                     /locus_tag="ACHKCL_003636"
     CDS             complement(4390..5772)
                     /gene="atpD"
                     /locus_tag="ACHKCL_003636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5764521.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5799..6662)
                     /gene="atpG"
                     /locus_tag="ACHKCL_003637"
     CDS             complement(5799..6662)
                     /gene="atpG"
                     /locus_tag="ACHKCL_003637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5764522.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6713..8254)
                     /gene="atpA"
                     /locus_tag="ACHKCL_003638"
     CDS             complement(6713..8254)
                     /gene="atpA"
                     /locus_tag="ACHKCL_003638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5764523.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8267..8800)
                     /gene="atpH"
                     /locus_tag="ACHKCL_003639"
     CDS             complement(8267..8800)
                     /gene="atpH"
                     /locus_tag="ACHKCL_003639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5764524.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8815..9285)
                     /gene="atpF"
                     /locus_tag="ACHKCL_003640"
     CDS             complement(8815..9285)
                     /gene="atpF"
                     /locus_tag="ACHKCL_003640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5764525.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9347..9586)
                     /gene="atpE"
                     /locus_tag="ACHKCL_003641"
     CDS             complement(9347..9586)
                     /gene="atpE"
                     /locus_tag="ACHKCL_003641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5764526.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9633..10448)
                     /gene="atpB"
                     /locus_tag="ACHKCL_003642"
     CDS             complement(9633..10448)
                     /gene="atpB"
                     /locus_tag="ACHKCL_003642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5764527.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10457..10837)
                     /gene="atpI"
                     /locus_tag="ACHKCL_003643"
     CDS             complement(10457..10837)
                     /gene="atpI"
                     /locus_tag="ACHKCL_003643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5764528.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2757 a         3071 c         2797 g         2765 t
ORIGIN      
        1 tgttttacaa gcgattcttg aatgaacaaa acgtgctatt cattacaaag tacatcaatt
       61 atggcaattg tttgtcaatg attcgaggta tttaattact actacggtta aaacaagtgt
      121 tcaacggtgc cccaaataat agctgaagtt aattatatgg aatgaggtta gattattaat
      181 gctggtaagt aaaagggccg ccaaaaggca gcccttcgtt cagcaaatga ataatattac
      241 tcaaccgtta ccgattttgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc
      301 gcgacgtgat aagccagtaa ctgcagcgga acggtgtaga agatcggtgc aatcacctct
      361 tccacatgcg gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctgc
      421 tcggcgaaga catacaattg gccgccacgc gcgcgaactt cttcaatgtt ggatttcagt
      481 ttttccagca attcgttgtt cggcgcaacg acgataaccg gcatatcggc gtcaatcagc
      541 gccagcggac cgtgtttcag ttcgcctgca gcgtaggctt cagcgtgaat ataagagatc
      601 tctttcagct tcaatgcgcc ttccagcgcg attgggtact gatcgccacg gcccaggaac
      661 agcgcgtgat gtttgtcaga gaaatcttct gccagagctt caatgcgttt gtcctgagac
      721 agcatctgct caatacggct cggcagcgcc tgcagaccat gcacaatgtc atgttcaatg
      781 gaggcatcca gacctttcag gcgagacagc ttcgccacca gcatcaacag cacagttaac
      841 tgagtggtga atgctttagt ggatgccacg ccgatttccg tacccgcgtt ggtcatcagc
      901 gccagatcgg attcgcgcac cagagaagaa cccggaacgt tacagattgc cagcgaacca
      961 aggtaaccca gctctttcga cagacgcagg cccgccaggg tatccgcggt ttcgccagac
     1021 tgtgacaagg tgatcatcag gctgttacga cgcacggcag atttacgata gcggaattca
     1081 gaggcgattt cgacgtcgca cggaatacct gccagcgatt caaaccagta acgggaaacc
     1141 ataccggagt tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc
     1201 agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg
     1261 gtaagggtgt ttttgatcgc gttcggctgt tcgtagatct ctttctgcat gtagtgacgg
     1321 taaatacctt tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact
     1381 tccgcgccag ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc
     1441 tcttcaagga agataaagcg acgggtcacc ggcagcagcg ccagctggtc agaagcgata
     1501 aagttttcac ccatacccag accaatcacc agcggactac cagaacgtgc tgccagcagg
     1561 gtatccgggt gacgggagtc catgatcact gtaccgtacg caccacgcag ttgcgggata
     1621 gcacgcagaa cagcctcacg cagagtaccg ccctgtttca gctcccagtt caccagatgg
     1681 gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttcg
     1741 cgcagcggtt catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca
     1801 tgcggatgcg cattcacttc tgaaggttca ccgtgggtcg cccagcgagt gtgagcaata
     1861 ccggtgccgc catgcagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg
     1921 aggcgacgca ggcgggtcat atgaccttcc gcatcaacaa cggccagacc ggcagagtca
     1981 tatccgcggt attccagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc
     2041 gcgatcgcgc caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca
     2101 acctgtatgc ccgttttttc gggcgccccg agccttgtag agagtggggt tatttttata
     2161 gttactgctt gtgggaggga gattatgtta tctcctcatc ccatgtgacc ggattacccg
     2221 gccagaatca ctttttcttt accggacgac gccagccttc tttctgagtc tgcggcacac
     2281 ggctgatagc taatgcattt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg
     2341 tcgcgccttt gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat
     2401 cgtcgccgat aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg
     2461 cgccgatgtt aacgttatcg ccaatttccg catcgccaag gtaagtcaga tgaccagctt
     2521 tcgagccttt acccagacgt gcttttttca tctcaacaaa gttaccgacg tgtgcacctt
     2581 ccaacaactc agcaccagga cgcaaacggg caaacgggcc aatggtacag gccgccgcca
     2641 gattcgcgtc ttccacgacg gtatatgggc taatttcgca atcatcgcca atcacgctgt
     2701 ttttaatcac gcaaccggtg ccgattttca cgcgatggcc gagagtcacg ttgccctcga
     2761 tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa
     2821 aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact
     2881 ggtaaacgcg ctccagacgg gagagttgca ggcggttatt cacgccttct acttcgctta
     2941 aacgttgcgg atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt
     3001 cggtgatgta gtattcgccc tgcgcattat tgttggtcag cttcgccagc cagcgtttca
     3061 tatctgcgcc gttagcgatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt
     3121 cggtggcatc tttgtgctca acaatgccgg taactttgcc gttttcacgg gtgatacgtc
     3181 cataaccggt cggatcatcc agtttcaccg tcagcagacc aatgccaccc tgcggtttag
     3241 catcacgcag acgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta
     3301 aaatgtcttc atcatcggca aagaaaggtg cggcctgctg catggcatga cccgtaccca
     3361 gctgctctgc ctgaagcacc cagttcaggt tgtcgtcttt cagcgcctgt tttagcagat
     3421 cgccaccgtg accgtacacc aggtgaacgt gcgctgcgcc taattcattc gcagcatcaa
     3481 tgacatgctg aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat
     3541 acatgcgcgt gcctttacct gcggcaagga tcactacgct catagcatta ttcaacatac
     3601 gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt
     3661 ttttcatcgt gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga
     3721 aagcgccatt ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc
     3781 atttaagcgt caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca
     3841 gggggtttta tatcagcagg atctatgtga acgctattca ggacgggtca cacgcgcaaa
     3901 aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt tacatcgctt
     3961 ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc gcagacgcct
     4021 gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta cgtttcgctt
     4081 ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc agaacggtca
     4141 cgttgccaag ctgcacttca agaatgccgc cagacagata gataaactct tcgtgaccgt
     4201 gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt gcgtggccag
     4261 ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc agaccagaga
     4321 acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata tcaccctccg
     4381 attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac cgaccatgta
     4441 gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa agccacggat
     4501 ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg ccacgaagaa
     4561 cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt tgtcttcttc
     4621 agacagttca tccataccca ggatggcgat gatgtctttc agttcctgat aacgttgcag
     4681 gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca gcgggtccag
     4741 ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag acgcgatctg
     4801 acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag acgggtcagt
     4861 caagtcatcc gcaggtacgt atactgcctg tacggaggtg atagaaccgg ttttggtgga
     4921 ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat aacctaccgc
     4981 tgaaggcata cggcccagca gtgcggatac ttccgtaccg gccagggtgt aacgatagat
     5041 gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag ccatggtcag
     5101 accggtcaga gctacgcgca gacggtttcc cggcggctcg ttcatctggc catacaccag
     5161 ggatacttta tcgataacgt tggagtcggt catttcgtgg tagaagtcgt tcccctcacg
     5221 agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg cgatgttacg
     5281 aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca gaccaacttt
     5341 accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt ccagcagttc
     5401 ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc aacgctcttc
     5461 ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga tacggcccag
     5521 agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat ccagaccgcg
     5581 acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccgc cgagctgctg
     5641 ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat cgtacacgcg
     5701 cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct ggacaatctt
     5761 tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc ggcccccgag
     5821 acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta taccaactgc
     5881 agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac catacgggcg
     5941 gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc gacataacga
     6001 cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc ccaggattta
     6061 tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat ggtcggaacc
     6121 tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag acggccttcg
     6181 tcgtaggcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga agggttatcc
     6241 cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa cgacacgcct
     6301 ttcgagccga tcattgcgag gtcgcattga acgcctttgt cggtccaggt cttcatttcc
     6361 gccagcagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg gtcggtcgac
     6421 accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg cttatattcc
     6481 agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata aggacggctg
     6541 gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc catcgcttta
     6601 gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt tgcgccggcc
     6661 atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga cgttaccagg
     6721 attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt tcgtcgttgt
     6781 agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg acgtaagcca
     6841 gcagagcggc ttcgaagctg ccgattttcg acagttcaac atccgccagg taaccacgtt
     6901 ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac tgtttctgtt
     6961 tcagcagttc ggtcactttt tgaccgtggt caagctgctt acgtgttgca tcgtcaaggt
     7021 cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg gtacggatac
     7081 caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg gatacggaaa
     7141 tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc aggaagatct
     7201 gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca cccgcttgag
     7261 tttcgataat cggcagcgcg gtcagtgaac cggttttccc tttcacttca cctttggtga
     7321 aagcttcaac gtattcggcg ttaacacgcg cagcacgctc cagcagacga gagtggaggt
     7381 agaatacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc agcgagatct
     7441 gacggtaagc aacagcctgt ttagacagat catcgtaaat gatcagcgca tcttcaccgc
     7501 ggtcacggaa gtattcgccc attgcgcaac cggcatacgg tgccaggtat tgcagtgcag
     7561 cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg tgctcttcca
     7621 gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca tagatacatt
     7681 tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt ttaccggtct
     7741 gacggtcacc gatgatcaat tcacgctgac cacgaccgat tgggatcatg gagtcaacgg
     7801 ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg cccggagcga
     7861 ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg attggtgcac
     7921 ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc aggatacggc
     7981 cagtacactt aactttcatg ccttcggcaa ggtcagcata cggacccata acaaccgcac
     8041 caacagagtc gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc agggagatca
     8101 tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca cttacagaaa
     8161 caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg cgctgcttga
     8221 tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa gactgcaaga
     8281 cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata tcacccgctc
     8341 ggatgataac gcctgccatt acagacttat cgattttgca attcagctta actttgcgtg
     8401 acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc agtgcggcag
     8461 cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga ataaactgct
     8521 ccaaaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga ataaggttct
     8581 gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggca agcgtttctg
     8641 gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc tcggcggcaa
     8701 acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag tcaaaagctg
     8761 ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc ctccttacag
     8821 ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg aacgttcgat
     8881 gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct cttcacgggc
     8941 acgtttacgc tcggcttcaa tttccgcctg cgcctgggcc acgattttag tacgttcctg
     9001 ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg cctgctcgat
     9061 gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg cctttgcaag
     9121 gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt ctttttgacg
     9181 tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca gaacgaacag
     9241 gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac aatgcctcta
     9301 tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac gcgacagcga
     9361 acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc agacccataa
     9421 cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct gcgccttcca
     9481 ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc agacccatca
     9541 tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca gtctccagtt
     9601 tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc catcgacaga
     9661 tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag gatgtggaaa
     9721 atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc agcaatcaga
     9781 atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccgag tgaaactggt
     9841 ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc ccagtgattg
     9901 aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt gatgctgtag
     9961 aacagaatca ggataaatac gcccagtgcc atagacagcg ttacgttcac gtccgcagac
    10021 ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg cagcaggtcg
    10081 ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt cagggccagc
    10141 ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt aacaaagccg
    10201 atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc ctttttggct
    10261 acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa catggagtca
    10321 atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga gaatgtacgc
    10381 aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt catattttct
    10441 gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt atctgaacca
    10501 ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa accgccaacg
    10561 ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcaccaaat gcgaatgtcc
    10621 aggccacccg gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat atcataaaca
    10681 aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc cagaaggggt
    10741 ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg agcagaagct
    10801 tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact cctgctccct
    10861 tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa gcatcaaaaa
    10921 gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca aaaggtgaat
    10981 aaatgtttaa atatttttcc agtgcataca attgcgactt ttctactaac cctgttcgat
    11041 catgaaaaac tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca gatcacatat
    11101 tgcgcatgtt cgcgcacagc atatttattt acttggcaaa tgatgccttt gcaagtttat
    11161 gatatttcag tctaaaaaca gatactgttt taataaatga catttacaca acaaaaacca
    11221 cccattgaca tttttaataa tgttttaaca gccaatgatg gttcttagcg ccgattttta
    11281 gcagactgat attttcacta atgacttatt ttctgcttac caaaaaaagc cacgttatct
    11341 tgttgatgca aaagagtgaa cgtggcgtta aatgtaacca gttatatcag
//
LOCUS       DAZFKY010000154        11359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFKY010000154 DAZFKY010000000
VERSION     DAZFKY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            100..234
                     /locus_tag="ACHKCL_003644"
     CDS             100..234
                     /locus_tag="ACHKCL_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5764529.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            213..317
                     /gene="speFL"
                     /locus_tag="ACHKCL_003645"
     CDS             213..317
                     /gene="speFL"
                     /locus_tag="ACHKCL_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO5764530.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            628..2826
                     /gene="speF"
                     /locus_tag="ACHKCL_003646"
     CDS             628..2826
                     /gene="speF"
                     /locus_tag="ACHKCL_003646"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5764531.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2823..4142
                     /gene="potE"
                     /locus_tag="ACHKCL_003647"
     CDS             2823..4142
                     /gene="potE"
                     /locus_tag="ACHKCL_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5764532.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4199..5839)
                     /gene="pgm"
                     /locus_tag="ACHKCL_003648"
     CDS             complement(4199..5839)
                     /gene="pgm"
                     /locus_tag="ACHKCL_003648"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5764533.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5865..6410)
                     /gene="seqA"
                     /locus_tag="ACHKCL_003649"
     CDS             complement(5865..6410)
                     /gene="seqA"
                     /locus_tag="ACHKCL_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5764534.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6595..7359
                     /gene="ybfF"
                     /locus_tag="ACHKCL_003650"
     CDS             6595..7359
                     /gene="ybfF"
                     /locus_tag="ACHKCL_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5764535.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7499..7792
                     /gene="ybfE"
                     /locus_tag="ACHKCL_003651"
     CDS             7499..7792
                     /gene="ybfE"
                     /locus_tag="ACHKCL_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5764536.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7932..8462
                     /gene="fldA"
                     /locus_tag="ACHKCL_003652"
     CDS             7932..8462
                     /gene="fldA"
                     /locus_tag="ACHKCL_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5764537.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8672..8758
                     /gene="uof"
                     /locus_tag="ACHKCL_003653"
     CDS             8672..8758
                     /gene="uof"
                     /locus_tag="ACHKCL_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5764538.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8751..9197
                     /gene="fur"
                     /locus_tag="ACHKCL_003654"
     CDS             8751..9197
                     /gene="fur"
                     /locus_tag="ACHKCL_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5764539.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9281..9607)
                     /gene="chiQ"
                     /locus_tag="ACHKCL_003655"
     CDS             complement(9281..9607)
                     /gene="chiQ"
                     /locus_tag="ACHKCL_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5764540.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9657..11062)
                     /gene="chiP"
                     /locus_tag="ACHKCL_003656"
                     /pseudo
     CDS             complement(9657..11062)
                     /gene="chiP"
                     /locus_tag="ACHKCL_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2780 a         2789 c         2815 g         2975 t
ORIGIN      
        1 tattatttgg caggtttaat ttcttaacgc aaatataaaa cacgaaatta caacatttaa
       61 gtaacaaaac agcatctagt atggcgctat tcataattaa tgatttatta tctggggtat
      121 gatcgttttt tgttgatctt cttcacagat tatagccatt tcatggatag aataactcta
      181 ccttcaactg acacagcaag aggtaaaggt aaatggaaaa taataaccgc ttaatgcctc
      241 atataaggcg gacaacccat atcatgaagt ttgcccatcg taatagtttt gactttcatt
      301 tctttaatgc ccgctagtct tctgactaaa gggcacccca gcgtacaggt ctccctgact
      361 ttaagcatta caggttaata cctgtatccc tcggtgctca tatactgcta acccttttaa
      421 actctacata attcgagtcg cagcatatgc aacttgaagt atgacgagta tagccagtta
      481 ccgggctggt ctgggttatt gcatctgcaa aaagcaaact actgatttat ttatcagcgg
      541 tggagctttg ctttttttcc ggcgtgatcg atttctcctt tgagaaattg aggacctgct
      601 attacctgaa ataaagagat gaacaaaatg tcagaattaa aaattgccgt tagtcgttct
      661 tgcccggatt gtttttccac tcatcgtgca tgcgtgaata tagacgaaag taattatatt
      721 gacgttgccg ccattatttt atcagtcagt gatgttgaac gtggaaaact cgatgaaata
      781 gacgctactg gctatgacat tcctgttttt attgcaacgg aaaatgaaga acgtatacca
      841 gcagaatatc tttcacgtat ttctggtgtc ttcgaacatg gagaagcacg caaagaattt
      901 tatggtcgtc agttagaaac cgctgccagc cattatgaaa ctcaactgcg cccacctttc
      961 tttcgcgcac tggtcgatta tgtcaatcag ggcaacagcg catttgactg ccccggtcat
     1021 cagggcggcg aatttttccg tcgccatccg gcggggaatc agtttgtgga atactttggt
     1081 gaggcgctgt tccgtgccga cttgtgcaac gccgacgtag cgatgggcga tctgctgatt
     1141 cacgaaggcg cgccatgcat tgcacagcaa catgcagcaa aagtgtttaa tgccgataaa
     1201 acctacttcg ttttaaatgg cacttcatct tctaacaaag tggttttaaa cgccctgctg
     1261 acaccgggtg atctggtgct atttgaccgc aataaccata agtctaacca ccacggggcc
     1321 ttgctacagg ctggtgcaac accggtttat ctggaaacag cacgtaaccc gtatggtttt
     1381 atcggtggta ttgatgcaca ctgcttcgaa gaaagttatc tgcgtgaact gattacagaa
     1441 gttgcaccgc aacgggcaaa agaggcgcgt ccgttccgcc tcgctgtgat tcagctaggc
     1501 acctacgacg gtacgattta taacgcccgc caggtggtgg ataaaattgg tcatctgtgt
     1561 gactacatcc tgtttgattc cgcatgggtc ggctatgaac agtttattcc gatgatggct
     1621 gactgttcgc cgctgttgct ggatcttaat gagaacgatc cgggtattct ggttacgcaa
     1681 tccgtacata aacagcaggc tggtttttct caaacttcac aaatccataa aaaagacagc
     1741 cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc ctttatgatg
     1801 catgcctcca ccagcccgtt ctatccgctg tttgccgcac tggatatcaa cgccaaaatg
     1861 catgaaggtg tcagcggtcg taatatgtgg atggattgtg tggtaaatgg cattaatgcc
     1921 cgcaaactga tcctcgataa ctgtcagcat attcgtccgt tcatacctga actggtggat
     1981 ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg cttcttccag
     2041 tttgtaccag gtgagcactg gcattctttt gaaggctatg cagagaatca atactttgtt
     2101 gatccgtgca aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa
     2161 gcgttcggtg tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg
     2221 gaaaaatgcg atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaaa
     2281 cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac tgctggaatc tgatgcgccg
     2341 ctggcggaag tgctaccttc catctacaaa cagcatgaag agcgctacgc cggttatacc
     2401 cttcgtcagt tgtgccagga aatgcatgat ttgtatgccc gccacaacgt gaaacaactg
     2461 caaaaagaga tgttccgtaa ggaacacttc ccacgcgtca gcatgaatcc gcaagaagct
     2521 aactacgcct atttacgcgg tgaagtggag ctggttcgtc tgccggatgc agaaggccgt
     2581 atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt tccgggtgaa
     2641 atctggggtg gtgctgtttt gcgttacttc agcgctctgg aagaagggat caacctgctg
     2701 ccaggttttg caccggagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa
     2761 gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca ccctgctgaa aggggaaaaa
     2821 ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata ctgacgatgg
     2881 tcaacatgat gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gttgggacaa
     2941 tctcaattat ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg
     3001 caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg cggttatgcc gagtatgctt
     3061 tcggtaaatc cggtaacttt atggcgaact atacctacgg cgtctcactg ctgattgcta
     3121 acgtcgcgat tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt
     3181 cgccagtgca gattggtctt gcgaccatcg gggtgctgtg gatttgtacc gtggctaact
     3241 ttggtggtgc gcgcattacc gggcaaatca gtagcattac tgtgtgggga gtcattattc
     3301 cggtcgtcgg tctgtgcatt attggctggt tctggtttag cccgacgctg tacgttgatt
     3361 cctggaatcc gcatcatgca ccgttcttca gtgcggtagg ttcttccatc gctatgacgc
     3421 tgtgggcttt tcttggtctg gagtctgcgt gtgcaaatac tgatgtagtg gaaaacccgg
     3481 aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg atttatatcg
     3541 tctccaccaa cgtgattgcc gggattgtgc caaatatgga gctggcaaat tcaacggcac
     3601 catttggtct ggccttcgcg cagatgttca cgccggaagt gggtaaagtc attatggcgc
     3661 tgatggtgat gtcctgctgc ggttcgctgc ttggctggca gttcaccatt gcccaggtgt
     3721 ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt ctcccatgta accaaagtgg
     3781 atgcaccggt gcagggaatg ttgaccattg tgattatcca gagcgggttg gcgctgatga
     3841 cgattagccc gtcgctaaac agtcagttca acgtgctggt taacctggcc gtggtgacca
     3901 atatcattcc gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg
     3961 tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg atgtacagct
     4021 tttatgcgct gtactcatcc ggggaagaag ccatgctgta cggttccatc gtgaccttcc
     4081 ttggttggac actgtatggt ctggtatcac cacgctttga actgaaaaat aaacacggtt
     4141 aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgccct ttttcttatt taatgtgttt
     4201 acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat
     4261 gttcttcacc gaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
     4321 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccaatagaa gcaccgttgc
     4381 ccggagcagc agtcaggcgc gcggtgatcg ggtcaccagc cagggtgctg gcactcacca
     4441 tttccggaga cagcttagac agcgccgctt tttgtgcgga agtcgcagct gcctgcaaac
     4501 ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct
     4561 taccggtgac agcggtgatt tccgccgcca gcagacacat gatgatgccg tctttgtcgg
     4621 tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa
     4681 agccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc gggacttcta
     4741 ccagtttacg gcccaagtcg ttgaccacac ggtcgatcat cgcagatgaa accagcgttt
     4801 taccgacggc aacagctttg ccccactgcg gacgatgctg gaacaggtag ttgattgcca
     4861 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
     4921 aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc aggcccgcca
     4981 tcgcacactc ggaggagcag tccatacgga tcgcgccgtc tttatcaagg tgcataaagc
     5041 ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa
     5101 tacgcttcca gtattcgata ccggaaccgc ccagcggatc aacgccaagc gtcaggcccg
     5161 ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacaaac ggctgcacca
     5221 gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
     5281 ctttcaggcc atcggccatc agtgcgttgg ctctgtcttc caccacttta gtgacgttgg
     5341 tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat
     5401 gggacggtgt aataacgata ccgtctgcca gcgggccacc ttttttattg tgaaccagga
     5461 tggcattgga aacggcaggc gtcggggtga agccattgtt ttcctgcaca ataacatcta
     5521 cgccgttcgc tgccagcact tccagaacgg aaataaatgc aggttcggac agggcgtgag
     5581 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
     5641 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
     5701 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
     5761 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
     5821 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
     5881 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
     5941 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgcctgg cacatgtttc
     6001 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt
     6061 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
     6121 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcata ttcatccgaa
     6181 agtagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgatcgg cttcgcttcg
     6241 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
     6301 gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
     6361 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
     6421 tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc ggcagcggat
     6481 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
     6541 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
     6601 ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
     6661 catggtctgt ttggcagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
     6721 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga tccggtaatg
     6781 aattacccgg cgatggcgca ggatcttgtt gatactctgg atgcacagca gatcgacaaa
     6841 gcaacattta tcgctcactc catgggcggt aaagcggtaa tggcacttac tgcactggcc
     6901 cccgatcgca tcgataaact ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc
     6961 cgtcatgatg agattttctc ggctatcaac gcggtcagtg aatcggacgc acaaactcgc
     7021 cagcaagcag cagcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg
     7081 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat
     7141 ccgcatattg tgggttggga gaaaatcccg gcatgggatc accctgccct gtttatccct
     7201 ggtggtaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
     7261 caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
     7321 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
     7381 cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg
     7441 cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat
     7501 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
     7561 acgaccgaaa acaaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca
     7621 actaaaacgc gacaaagtac gtggcctaaa gcgtgtcgaa ctgaagctga acgcggaagc
     7681 tgtcgaggcg ctgaacgagc tggcggaatc gcgcaatatg agccgtagcg aactgatcga
     7741 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
     7801 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
     7861 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
     7921 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
     7981 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
     8041 caaaaagcag caaagaagat ctggaagctt atgacattct gctgctgggc atcccaacct
     8101 ggtattacgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
     8161 atttcaatgg caaactggtt gcgctgtttg gttgtggtga ccaggaagat tacgccgaat
     8221 atttctgcga cgcattgggc accatccgcg acatcattga accgcgcggt gcaaccatcg
     8281 ttggtcactg gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg gcagatgacg
     8341 accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc gctgaacgtg
     8401 tagaaaaatg ggttaaacag atttctgaag agttgcatct cgacgaaatt ctcaatgcct
     8461 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
     8521 attgctacaa atttgtaact tttgccgttg tacctgtaca atgtcccggt gttcaagtgg
     8581 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
     8641 gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
     8701 tgtcacttct tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
     8761 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg
     8821 aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
     8881 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
     8941 acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
     9001 agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggttatc gaatttagtg
     9061 atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta
     9121 accacagtct ctatctttac ggtcactgcg ccgaaggcga ttgccgcgaa gatgagcacg
     9181 cgcacgaagg caaataagcc agtctgaacg agaaaagcca acctgcgggt tggctttttt
     9241 atgcaaggga aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgaa
     9301 tttcctgcca gaccttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg
     9361 cacgcagaca ctgctgataa tccagtacgc ggacactttc ctggtcggca aactgctgat
     9421 gttgtttctc tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttccggcg
     9481 aaccttgtgc ggtgttgata caggcgctgt atgcctcttt cagacggctg tcttcctgtg
     9541 gcgcggttga ttgcgcacaa gctaccagcc ccgatgccat catggcgatg agaatcagtt
     9601 ttttcatagc aaattcctca aacgtgcggc aggcacaaaa cctgccgcgt cgggcatcag
     9661 aagatggtga atggtgcgat taccataaat tttacgtcac gctcatcctg gaagatgttg
     9721 ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt
     9781 ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc caggctgtat
     9841 gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgccaggtc
     9901 gcaggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc tggaagattc
     9961 cagtttttca ggtcatacat cgcaccgaag aagaccgctt tttcgccgtt ggcgttgaag
    10021 tcagaacggt tatcccacca gatatccagg cgaccgttcg aggaagcgta ggttggagtc
    10081 atacgttgca ggaagtatcc ctgctgaccg tcagctttaa cccaggtgcc ttcgaggcgc
    10141 aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca ggcggtgccg
    10201 tcataaagat cgttgacgct gcgatcgtca actttatcgc gcgtaccgta gaactggtag
    10261 ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc aaagtattga
    10321 tcgatatacc cttccgcctg accaaatgcc gcttccagta cgaagttatt tttgaagtcg
    10381 tatttcgccc caagggagtg cagataatca actttggtgg ttttatcgtt ctgataaaac
    10441 tcatccattt ccagatgcca cggtgctttg tattcgttgg tccacatgta ggagaaactc
    10501 aacgcaccag catcgccgta atcaaaattc gccccggcct ccgcaccctg ataagtaccc
    10561 ggcataaagc tccagtgcgg cgctaacagc gtttgaccag ttggctgaat ataacctgcc
    10621 ctcgccacac cggaccgtat ttaaatttgg ccgctgcttt atacaggctt ataccgcttt
    10681 tatcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg atttcgttcg
    10741 ggtgagagct gtcgccgttt tccgccattt caatcgccgt aaacgcggca atatcaaggc
    10801 cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcgttccag gtggagtgag
    10861 aaaggttggt tttgtattta tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc
    10921 agtaatagat accgccggtt aaggttgaat cgtcaatgaa tccttcggcg cgagcctccg
    10981 gcattgccat aaagcccgac attgctgtaa caccggcgat agccagcgcc agcgtactac
    11041 gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg ctgcaccaca
    11101 ggtgaacagc gaaaaaaata aaaattaaaa aagtagcgtt gagaaaagtc tcgctgacta
    11161 caggaacaga ctatttttcg cgacgcgaat tatcaatctc tttctgtaac cagaatgtaa
    11221 gaacatgagc aaccgcacat attttgatga tttttgttac aaggcttaag tttgcggttg
    11281 aaaggcttgc agaagaataa aagcgtgaaa ttccagcaag atgaaagcgg ttacatttta
    11341 tctaattatg ttttgtgtt
//
LOCUS       DAZFKY010000155        11335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFKY010000155 DAZFKY010000000
VERSION     DAZFKY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(225..491)
                     /locus_tag="ACHKCL_003657"
     CDS             complement(225..491)
                     /locus_tag="ACHKCL_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5764541.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(488..1255)
                     /gene="yjiL"
                     /locus_tag="ACHKCL_003658"
     CDS             complement(488..1255)
                     /gene="yjiL"
                     /locus_tag="ACHKCL_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5764542.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1265..2416)
                     /locus_tag="ACHKCL_003659"
     CDS             complement(1265..2416)
                     /locus_tag="ACHKCL_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5764543.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2532..3803)
                     /locus_tag="ACHKCL_003660"
     CDS             complement(2532..3803)
                     /locus_tag="ACHKCL_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5764544.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3844..5076)
                     /gene="mdtM"
                     /locus_tag="ACHKCL_003661"
     CDS             complement(3844..5076)
                     /gene="mdtM"
                     /locus_tag="ACHKCL_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO5764545.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5543..6325
                     /locus_tag="ACHKCL_003662"
     CDS             5543..6325
                     /locus_tag="ACHKCL_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5764546.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6300..6500
                     /locus_tag="ACHKCL_003663"
     CDS             6300..6500
                     /locus_tag="ACHKCL_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764547.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6743..8155)
                     /locus_tag="ACHKCL_003664"
     CDS             complement(6743..8155)
                     /locus_tag="ACHKCL_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5764548.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8332..8496
                     /locus_tag="ACHKCL_003665"
     CDS             8332..8496
                     /locus_tag="ACHKCL_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5764549.1"
                     /translation="MEFHENKAKTPFIRLVQLRQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8593..9474
                     /locus_tag="ACHKCL_003666"
     CDS             8593..9474
                     /locus_tag="ACHKCL_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO5764550.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9522..10685
                     /locus_tag="ACHKCL_003667"
     CDS             9522..10685
                     /locus_tag="ACHKCL_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO5764551.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(10732..11073)
                     /gene="symE"
                     /locus_tag="ACHKCL_003668"
     CDS             complement(10732..11073)
                     /gene="symE"
                     /locus_tag="ACHKCL_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5764552.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2966 a         2811 c         2862 g         2696 t
ORIGIN      
        1 tttaacatta caacacccgg taatcatttt atttctctat gcatgattat gggtttaagt
       61 ataattcaat gtatattgta ttttagacgc gcaataggaa ggtgctatca aaaatcgctc
      121 attttgtgga tgggaaatat ttcacggcag gcgagatatt tactacaact tccttcatgc
      181 aggattatgc gcagccaatt atccggttgg tatatttaaa gatcctattg cctggttgac
      241 gcttctgcat cgtaatgcac cacgcactgc cagtcgctgc cctgctggca atatatcgct
      301 tcggtttgcc cagggatcac ccattgtatt tcctcgccgg ggggacaggt cagccaaatg
      361 cataacccgc agccgccgcg taaatcacgg ggaatatcgc tgacgcgaaa agtcatgccc
      421 gctgcctgca acgccttgcg ggtttgtatg acgccgaccg tggagtgaaa taaaaataaa
      481 aactctttca tcggcgtgtc ctcactcgtt gaccaattac cgccgcgcca attgtgccag
      541 caaattgcgc atcaggatgg gtatttaccg gcattcgcag gtgagattcc agcatccggg
      601 caaacgtctg gcaatgacta acgccaccag taaacagaat cggcgcttca caggagagac
      661 gagcaatgaa attggcactc cgccgcgcca tcgcgttaat cactccggcg agaatcgctt
      721 ctggcgcgac gcccgctgag cgcaggctga tcacttctga ttcagcaaac actgtgcaca
      781 tactcgtgat ggcgtgcggc gtgacatttt cggtaatgca gtcgagttgc tcgacgctgg
      841 tgccaagcgt gcgcgagatc acctccagga aacgcccggt gcccgccgcg catttgtcat
      901 tcatcaggaa atcgcacagg ttaccgtcat catcaagctg aatcactttg ctgtcctgac
      961 caccgatgtc gattaccgcg cgcgttgctg gcgcaagaaa ccgtgcgccc agcccgtgac
     1021 aggagatttc cgtcacttgt ttatcggcaa aatccaccag ttgccgcccg tagccggtga
     1081 gcgtcagaaa cggcgttgtc tctaaccctt cgcgcagagt ttcccaggct tcagtaattg
     1141 ctgttgccgg tcgaaagggt gttggaacga ggaaacggcg cgtaatcacg ccgtctgcga
     1201 gtaagatccc tttggtggcg gtagaaccag aatcaatgcc aatcgaatat gtcactgcta
     1261 ctccttacag catctcaata aaggccgcga cacgggtact gagctgccca acatccgagg
     1321 tggagtagtc tgtttcaata gcgatataag gaatgttgtg ctgctgacgc acatgacgtt
     1381 taattgccag cgattccacc gcgtaggtat ggcacgcctg caaaatcaca tcaactacgc
     1441 catcgacctg atattcttcc accatctggc tgagcatttt caggcgctga tcgttcggcg
     1501 aaacacagga gcagccaatc gccagatatt tatccgccag cgcgtcgtag acatcgcccg
     1561 tttctgccac gcattgctcg gtcgctttcg ccccggtgca gttttcataa ccgacaaccc
     1621 agccgccatt ctcttcaatc gcgcgcacca ctttctctgc tgcgccgcca atcgggcagc
     1681 cggtgattaa aatgcgcgga cgcgggtcca gtcgctggcc ttcttcccac tgctgacgaa
     1741 tgcgggcggt catcgcgtcc agttcattga tcaacgcctc tttatcgaac cggaaggttg
     1801 cgccgtaaac cactttcaga atgtcgctgc cgctaagcgc cggaggattg aactgcccaa
     1861 gatgataaaa attggccagt gcgcgacgtt cgcggttttt cagcgcaatg gcatcgcgca
     1921 gagcatcttc gctaatttcg tgcccaaaac gttcttccac cgctttttgt aagcgcagca
     1981 tctcggcttt ccataacgca cgcgaggcat cgtctttaac gctgtttggc agctgcatca
     2041 catgaacggg cttaaactcc gccatgtatt catacatttt ctttttgccg tcgcaggtgg
     2101 tttcaccgac caccagatcc gaaaagtaga agtaggggca tttatcggtt ttgccgaagc
     2161 cgtagctact tttaatcagc gggcagaggt tgcgcggcag atctttctcc gcttcttcaa
     2221 tggtttcatc agaggtggaa cagagcgaaa ccacaaccgc accggctgcc atcgggatct
     2281 cttgcggcat aaaggtgcag taagtgccaa ccagcggaat gccgcgcacc ttgagatcca
     2341 tgacggtgag aaagcctttc tggcgagctt cagagaactg atcgaaaatg gcgggtagat
     2401 cggtgacaag tgacatgatt ttccttcccc gtaccacggg agataatgaa aagaggccac
     2461 attattacca ttcttttagt atggtttatt tgatctctct cgcggttagc cacttagttt
     2521 ttcatggatt tctaaaaatt gccgaggggg aacaaggccg ggagctgtga cagcaaatat
     2581 aaaattagcc caatacaacc gccaaccagc gtaccgttga cacggataaa ctgcagatct
     2641 ttgccgatat ttaactctat ttgccgcgac atatcccggg catcccagct ttttaccgta
     2701 tcgctgatat ggcgcgtcag gaatgcagaa aactcaggcg cgacgcggtg cgcggcttgt
     2761 tccaggtgac catttaacga cgcccgcaag gcatcatcgg caattaacgt ttcgccaaac
     2821 cattgacctg cccgcgcgat acgttctttc acacgagaat cttcactgtt gatatccgct
     2881 ttcagccatt cccgtaaatc cccccacaat tcactgagaa aggcttcatc ctctttcaga
     2941 tagcttttta cggcatcagc tcgcgctgtc atttcaggat cgtttttcaa cttgtcgatc
     3001 agggcaaaag tggcgcgatc aaatgcatgg cggatctgat gcgcacgatc gcggctgata
     3061 tcatcaagca aagaattcac cgcgtcagaa accaactccg cgctatgttc gcccagccat
     3121 tcggtgggga gaattttggc tttcagtgga tgctcactct ccagccagcg aacaatctgc
     3181 tgggcgataa acttgcgcga tttatcgcgc tggagaaggg cgatcaactg tgcgatcagc
     3241 gtatccagca gcacctgatg acgatcgttt ttggtcatac tctccagcat caacgcactg
     3301 gtgccggaaa gatcgacctt atcaatcgcc cgatggaccg cgcgcttaag caggcgctga
     3361 atacgtgcat catcggtcag ttcaagaaaa ccactcatga tctgcaacag atgctgacca
     3421 acgcggcggg cgttttctgg ctggctaaac cagttgccaa tcagcaacgc cggttcgtga
     3481 cgtcgaatca atgccaccag cgattgggta tcgagaaatt ttttctgcac gaactgaccg
     3541 agattttcgc caatccggtc tttattacgc gggataatcg ccgtatgacg agaaatgatc
     3601 ggaatcggca cgcggcgaaa cagcgccacc accgcaaacc agtccgccag cgcgccgacc
     3661 atcgccgctt cagcaatcgc cttcacgccg ctcacccaaa aattgggcgg caaaaacagc
     3721 gtaacgacaa aggtagcagc ggcgataagc agtaaagaga gcgccaacat tttggcgcgt
     3781 ctgagttcaa tgagtttatt cataacgtta agaatagagc ctgacggcag gatcgcgcaa
     3841 aaatcactgc tcctctgcca gctcggcagc ctgatgttgg cgcacgcgat taagcaatcc
     3901 ggccagggtg aaaacgacga taacgcccgc cacaacggct aacagatgaa acggcaagcg
     3961 accgccgtta aaccacagcc agcggccaat ttcgaccgag acagacatca ccatcaggat
     4021 caccatattc agcgatgcgg agacggtccc tttcggtaag ttattggaaa acagcgtaaa
     4081 gcggaataag gtcgggaaga tcaaaccaat cccgaaagca tacagactgg tgcccagcac
     4141 cgaccacagc cagacgtgcg gtgacagcag attgccgata atcaacagcg cgaggccgac
     4201 cagttgaatg ggtacggcac gccagataaa gcgcggttcg gtcggatctt taacgaaacg
     4261 cgccacgatg gcattcgcga caatcaccgc gccgaacacc ggaacctgtg tccaggcgaa
     4321 ctgcgaggtc gttaagccgc ctgcgtcaat caggatcacc ggcgagacag ccacccaact
     4381 catcatgggg atatagctta aagagatggt tgctgcgcca aagaggaaca gccgattgca
     4441 aaagacatta cgaaaatcgc gcaagacgct tttggcgcta aatggaaccg cgccgcgctt
     4501 caccgtctct ggcatcgcca gcagtaagcc aacaaatgag ataaaaccca taaccgcaat
     4561 gatggcaaaa aggactttcc agtgcacaaa gtgcatcaga gctgcgccgg aaagcgggcc
     4621 gataatcggc gcaatcagta cgatggaggt gataatcgcc atcaacttga tcccttttgt
     4681 ctggccgaac gcctcctgca ccgtgacata accaacggtg gcaataaaac agatactggt
     4741 gccctgaatt gcacgcgcga taagaaactg tgtcatagac gttgtgaaca ttgtcgcagc
     4801 gcaggcaagg gtaaatatta gcgccccggt aatcagcact ggcttgcggc caatcctgtc
     4861 ggaaagcggc cccagcagcc actgcaacgc catgcctcca gcgagataga gactgacggc
     4921 agccggagcc agactgacat cggcattaaa atcacggacc acattaatga tcccaggctg
     4981 gatcaggtcc gtcgaaagat aggcggcaaa gtcatacaaa atcaacgcca tcgggaaaaa
     5041 cagcgtggcg gcatggcggg caaaaaaacg tggcataaca aacaactcct tgtccggaga
     5101 accccaacgg ggaaaacgaa attcaatacc gtcggtaatg acaaaaggta ggcgagaagg
     5161 acaaatgtac tgaatttcat gtaatcaacc acactgcctg tgaacacaca gacagagaaa
     5221 taccttctga cttcgggtca gaatgtataa cgattacaaa tagtcatcag cacattacac
     5281 ctcttgaatt gatgggttgc atagaaaata tacaccttaa gtgtaattaa aatttgcgat
     5341 taatcaaaag agcgcggtgg aagggggaat atctgccggg tacaggacat gtaaaacgcg
     5401 agtcagtttc cggaaaaatg tatcgtttgc agacgaacaa aaatagcctg gaaagcgcct
     5461 cgggaaacga gaaattgccg ggtgagaatg gttttgttag tcgctaaagt cgggccatct
     5521 tatcaacagg tgacggatcg ccatgacaaa cttcacgacc agcacgccgc atgacgcatt
     5581 atttaaatct tttctcatgc accctgacac cgcgcgggat tttatggaga tccacttacc
     5641 caaagattta cgtgaactgt gcgatctcga cagcttaaaa ctggaatccg ccagctttgt
     5701 cgatgaaaaa ttgcgggcgc tacactccga tattctgtgg tcggtaaaga cccgggaagg
     5761 cgatggctat atctatgtgg tgattgaaca tcagagccgt gaggatattc atatggcctt
     5821 tcgcctgatg cgctattcca tggcagtgat gcagcgccat atagagcatg ataaacgccg
     5881 gccactaccg ctggtcatcc cgatgctgtt ttatcacggt agccgtagtc cttatccctg
     5941 gtctttgtgc tggttggacg aatttgccga cccgactact gcacggaagc tttataacgc
     6001 cgcgttcccg ctggtggata tcactgtcgt gccggacgac gagattgtgc agcaccgcag
     6061 agtcgccctg ttggagttga tccaaaagca tattcgccag cgcgatctga tggggcttat
     6121 cgatcaactg gtagtattac tggttacaga gtgtgctaat gacagccaga taactgcgct
     6181 gttaaattac attttactga ctggcgatga agagcgtttt aatgagttta tcagtgaact
     6241 aaccagtcga atgcctcaac acagagagcg aataatgacg attgcagagc gaattcataa
     6301 tgacgattgc agagcgaatt cataatgatg gatggctgtt gggaagggaa gaaggggaac
     6361 agcgcattct tcgattgttg ttgcagaatg gggcagatcc tgaatggata cagaagatta
     6421 ccggactttc ggcagagcaa atgcaggcat taaggcagcc cttgcctgag cgtgagcgct
     6481 attcatggct caagagctaa ccagagacgg atgacaaacg caaagcagcc tgatgtgcta
     6541 cgcttatcag gtctacaaag cccattcaat atattgaact ttaaagattt tgtagacctg
     6601 gtaaggcgtt cacatggcat ccggcatgaa caaagcgcac tttacctaag ccatgccgga
     6661 acgtggctat gatcctgcaa agataaaacg agtcacaggc gattcgtgag aataaatcag
     6721 gtgcttcgcc actaattcta atttattcca ttgcccgata cacggcctcg ccaatttgct
     6781 tcagcgcttc gcgataagtt tcgctgagtg gcaaagcaca gttgatgcgc aggcagttac
     6841 ggtatttgcc ggaagctgag aaaatcgagc ctgccgccac ctggattttc atgcggcaca
     6901 gctgccgcgt gacgcagacc atatcgacct gttcaggcaa ttctatccac agtaaaaatc
     6961 cgcctttcgg gcgcgtaata cagatttcgc agggaaaata ttcccgtatc cagcaggtat
     7021 aaagcgccaa attgcgctga tagatctgcc gcatccgccg gatatggcga tgatagtgac
     7081 cttccagcac aaacgttgcc gccgccattt gcgtggacgg cacattaaag ctgctgatgg
     7141 cgtatttcat atgcatcagt ttatcgtgat aacgccccgg tgcgacccaa cccacgcgca
     7201 ggcctggagc aatactttta ctgaacgagc tgcacaacag cactcgcccg tcgatatccc
     7261 aggaatgaat ggtccgcggg cgcggatact ccgttgccag ttcgccgtag acatcatctt
     7321 caaaaatcac aatatcatga cgctgagcga gagagagaac ggcccgtttg cgcgcgtccg
     7381 gcataataaa tcccagcgga ttattacagt ttggcaccag aatgatgcct ttaatcggcc
     7441 actgttccag cgccagttcc agcgcttcaa cgctgatgcc tgtttctgga tcggttggga
     7501 tttcaatcac tttcacgccc atgccgcgca gcatctgcat tgaaccgtaa taacaggggg
     7561 attcgaccgc gacaatatcg cccggtttac acaccgccat taacgctagc gacatcgagt
     7621 tatggcagcc gctggtgatg atgatgtcat cggcagtgac caccgagccg ctgtcgagca
     7681 tcaggcgggc aacctgctcg cgcaataccc gctgaccggc tagcaagtca taaccgagaa
     7741 cggtttgcag attatgctgc accacccggc ttagctcccg ccacagcggt ttcaggctgg
     7801 gcgtttcgac atccggcgtg cttttgctta acggaacaat ggaactgtcg ctatgcgcca
     7861 ccagcatatc cagcaccaga tcccactggg taatttccac tgggcgctgc accggacgcg
     7921 tcatcggcgg cactggcggc tgggctttac gttgtgcgac aaaataaccc gaacgcggct
     7981 gcggagtgat gagcttcatc gtctccagcg tctggtacgc ctgctgcacg gtgctgatgc
     8041 tgacgccgtg ctcctgactt aagctgcgca ccgacggcaa tttctccccg tgacgataca
     8101 gcccttgctc aatccgctcg gcaagtagag tcgccagatg ttgataacgc gtcatgctgt
     8161 attccttatt tggaccatac agaaagaaaa accggtacag atgaggctaa aaaaccggat
     8221 gcagatgctg ttttaacctg tctgtatgtt aaacaaaact aatatttgca tctgtattgt
     8281 gaactgccag aagacggatg atgaagcctc tgaaatatga ggaggcagca gatggaattt
     8341 cacgaaaaca aagccaaaac gccgtttatc agacttgtgc aactcaggca ggcagtgagg
     8401 cgttggcggc ggcaaatgca ggcccgacgg gtgttacagc agatgagtga tgaacggttg
     8461 agggatattg ggttacgcag ggaggatgtg gagtgagggg tatgttcgtc gcggataaac
     8521 aggatttctc tataacctcc ataaggaagt ggttattatt gcgaaatcat cgtttcacgg
     8581 aaagacgtca gaatgaaagc tacagaagct cgcctactcg attttttaaa acgttcgcag
     8641 cagtttgtta ttcccattta tcaacggact tattcatgga cagaacaaca atgtcggcaa
     8701 ctttgggacg acatcattcg tgcaggaaag cgtgacgata tatcagcgca ttttatcggt
     8761 tcggttgttt atatagagca gggattgtat caggtttctg gtatttctcc gttgctggtc
     8821 attgatggtc agcagcggct gacgaccgca atgttgctga ttgaggcttt atcgcgccat
     8881 cttggcgaag acgaagtttt tgatggcttt tcggcaatga aattgcgtaa ttattatttg
     8941 ctcaatcctt atgagtccgg cgagaaaggt tttaaattac tgctgaccga gactgataaa
     9001 gacagtttac tggcgttaat aaaacaaaga ccaatgccag aaaactattc ccatcgaata
     9061 atggaaaact ttactttctt tgatgaacaa attgccaaac tcggtgatga cttgatcccc
     9121 ttatgtcgtg ggttagcaaa gttattaatt gtcgatgtgg cgcttaatcg tggtcaggat
     9181 aatccgcaac tgatttttga aagtatgaac tccaccggta aggcgttaag tcaggccgat
     9241 ctggtgcgca attttattct gatgggcctc gaaccagagc atcaaacccg gttgtatgaa
     9301 gatcactggc gtccaatgga agtcgcttgt ggtcagcaag gttacagcga atattttgac
     9361 agttttatgc gtcattatct gacggtaaaa acggggagat ccctcggaca gatgaagtct
     9421 atgaggcatt taaactccat gcccgcagcc agagtgttgc tgaaaaaggc gtagatcggc
     9481 tggttgaaga tattcatatc tacgcggagt attactgtgc aatggcattg ggaaaagaaa
     9541 gtgacaaatc gcttgctacg gcttttcagg atttgcgcga gttaaaggtt gatgtggcgt
     9601 atcctttctt actggcgctt tatcatgact ataaaaatga cgatttgtct cacgaagatt
     9661 tcctgagcat aattcgttta attgaatctt atgttttccg ccgtgcagta tgtgcgattc
     9721 cgacaaattc tcttaacaag acgtttgcta ctttttataa ggtcattaat aaagaaaaat
     9781 atctggaaag tattcaggta cattttttga atctaccttc atatcgtcgt ttccccaacg
     9841 atgatgaatt taaacgggaa ttaaaagttc gcgatctcta taacttccgt agtcgtagct
     9901 actggttacg acgactggaa aacgataaac gcagagagcg cgtggaagag tttacgattg
     9961 aacatattat gccgcagaac gaaaatttgt cggctaaatg gcgcgaagag ctgggaagtg
    10021 actggcagcg tattcataaa gaactgttgc atacgttggg gaatctcact ttaacgcgct
    10081 ataactcccg ttacagtgac agacctttcg cagaaaaacg cgatattgaa gacggcttta
    10141 agcatagccc gctctatttg aatatcggtc ttggacagtg cgaaaaatgg gatgaagctg
    10201 ccattcacgc ccgagccgat cgtctggcgg agcttgcggt tcaggtctgg caagcgcctt
    10261 ctcttcctga agaggtttta gctgtttatc ggggacagcc tgagaacaaa accagttaca
    10321 gcctgagtga ttatcctttt cttgctgatg gttcgcatag ccgggtgtta ttcgatcatc
    10381 ttcgcgatga aattatgcgc ctggacgcag ggatcacgca ggaagtattg aagctctata
    10441 ttgcgtttaa agcggaaacg aattttgttg atgttgtgcc gcaaaaaagc cgactgcgat
    10501 tgtcgcttaa tatgcagttt catgaactgg tcgatccgaa aggtattgcc aaagatgtga
    10561 caaatgttgg gcgatggggc aatggtgatg tggaaattgg tttcagtgac ctcgcacaac
    10621 ttccttacat tatggggtta attcgtcagg catttgaaaa acagatggag agcgcgttgg
    10681 tataacgcag cgaaaaatgc ccggtagtcc tggcgctacc gggtcaatgt atcaggcaac
    10741 tttctgttta cccgcaatca ccccaataaa ctcctgcacc tgcctttgtt tacgcgccga
    10801 cagcttgcac acctggcgca gcgactgcat cagttcgctc tcggcggcgg cgggtggttg
    10861 ggcggtgagg acaatacagc cttccatcac tttgacatct accaccgtgc cggtggcaaa
    10921 accggcggct tccagccact gacctttcag ggtgatggcg ggaatacggc tgtaatccgg
    10981 gtaacgactc gcataactca cggttaattg acggttattt gccggggaga cttctgcttc
    11041 gaacggttgt gcaatagaat gcgtatcagt cataactgct attccctata aatagtgatt
    11101 gtgattagcg gtgcgggtgt gttggcgcac atccgcaccg cgctaaatac ctggatatat
    11161 catcagtaaa tatggggaaa gtccagctaa aaattaatga aaataagatg aaaattagcg
    11221 tgaaatataa attttggtga tgtgaataaa gcggctattt agccgcttta ttggtttttc
    11281 gagggcaggt aaattagttt accgccgcgt cagtgagtgc atctgcaagg tgtag
//
LOCUS       DAZFKY010000156        11205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFKY010000156 DAZFKY010000000
VERSION     DAZFKY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            202..1773
                     /gene="garD"
                     /locus_tag="ACHKCL_003669"
     CDS             202..1773
                     /gene="garD"
                     /locus_tag="ACHKCL_003669"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5764553.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1922..2257
                     /gene="prlF"
                     /locus_tag="ACHKCL_003670"
     CDS             1922..2257
                     /gene="prlF"
                     /locus_tag="ACHKCL_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5764554.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2257..2721
                     /gene="yhaV"
                     /locus_tag="ACHKCL_003671"
     CDS             2257..2721
                     /gene="yhaV"
                     /locus_tag="ACHKCL_003671"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5764555.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2776..3585)
                     /gene="agaR"
                     /locus_tag="ACHKCL_003672"
     CDS             complement(2776..3585)
                     /gene="agaR"
                     /locus_tag="ACHKCL_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5764556.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3834..5114
                     /gene="kbaZ"
                     /locus_tag="ACHKCL_003673"
     CDS             3834..5114
                     /gene="kbaZ"
                     /locus_tag="ACHKCL_003673"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5764557.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5137..5610
                     /gene="agaV"
                     /locus_tag="ACHKCL_003674"
     CDS             5137..5610
                     /gene="agaV"
                     /locus_tag="ACHKCL_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5764558.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5621..6400
                     /gene="agaW"
                     /locus_tag="ACHKCL_003675"
     CDS             5621..6400
                     /gene="agaW"
                     /locus_tag="ACHKCL_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5764559.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6390..7268
                     /gene="agaE"
                     /locus_tag="ACHKCL_003676"
     CDS             6390..7268
                     /gene="agaE"
                     /locus_tag="ACHKCL_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5764560.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7286..7720
                     /gene="agaF"
                     /locus_tag="ACHKCL_003677"
     CDS             7286..7720
                     /gene="agaF"
                     /locus_tag="ACHKCL_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5764561.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7717..8850
                     /gene="nagA"
                     /locus_tag="ACHKCL_003678"
     CDS             7717..8850
                     /gene="nagA"
                     /locus_tag="ACHKCL_003678"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5764562.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9201..10355
                     /locus_tag="ACHKCL_003679"
     CDS             9201..10355
                     /locus_tag="ACHKCL_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5764563.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10368..>11205
                     /gene="kbaY"
                     /locus_tag="ACHKCL_003680"
     CDS             10368..>11205
                     /gene="kbaY"
                     /locus_tag="ACHKCL_003680"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5764564.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCG"
BASE COUNT         2664 a         2903 c         2983 g         2655 t
ORIGIN      
        1 catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttaccttg ccgggcggca
       61 ttgcaatccc tgaaatgatt gacattgatc acatttctgc gtttaaactc ctgacattct
      121 tatttcaccc aatgaagtca tttattttta aatgagacca ggtcctcatt ttaataacct
      181 ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac gccaactgcg
      241 ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga taatggcctg
      301 aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatattcc ccaggggcat
      361 aaagtcgcat tgctcgacat tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt
      421 ggttacgccg tgcgtgcaat cccacgcgga agctggatcg acgaatcaat ggttgtactg
      481 ccgaaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccgga acccttaccg
      541 ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt gggcaccaaa
      601 aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga ctacgtagta
      661 aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg
      721 aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg cggcagttgt acctatccgt
      781 accattcaca atatttcgct gaatcctaac tttggcggcg aagtaatggt gattggcctg
      841 ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa cggatgatgt gcaagctatt
      901 ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg ttttcagtcc
      961 atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa tcaacgtcag
     1021 cgagaaacct gtccggcttc agaactggtt gtcggcatgc agtgcggtgg cagcgatgcc
     1081 ttttctggcg taacggcaaa cccggcggtt gggtatgcgt ctgatctact ggtgcgctgc
     1141 ggcgcaacgg tgatgttctc agaagtcacg gaagtgcgtg acgcgatcca tctgctgaca
     1201 ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga gtggtacgat
     1261 aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg caacaagaaa
     1321 ggcggtctgg caaatgtagt ggagaaagcg ctcggctcca ttgctaaatc gggtaaaagt
     1381 gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt aatttacgcc
     1441 gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg tatcaccgtg
     1501 caagtgttta cgaccggtcg tggtacgccg tacggcctga tggcggtacc cgtcattaaa
     1561 atggcgaccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat taacgcgggc
     1621 actatcgcca ccggcgaaga aaccattgaa gaggtgggct ggaagttgtt ccactttatt
     1681 ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatggggact gcataaccag
     1741 ctggcggtgt ttaacccagc accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac
     1801 atggccttag tgccatgttt ttattgttta aagcccccac gtccattaat aatgcatttg
     1861 cattacctta gttcaagctt ataatttgag cagaaaacag gctgtaaaag gacagtgaat
     1921 catgcccgct aatgctcgct ctcacgctgt actgaccact gaatcaaagg tcacgatacg
     1981 cggacaaaca actatccccg cgccagtgcg tgaggcctta aaactgaagc caggccagga
     2041 cagcattcat tacgaaattc tgcctggtgg gcaagtattt atgtgccgac tgggagatga
     2101 gcaggaggat catactatga atgcattttt gcgttttctg gatacagata tccagaacaa
     2161 cccgcaaaaa actcgtccat tcaacattca acaaggaaag aaacttgtcg ctggcatgga
     2221 cgtcaacatt gatgatgaga ttggcgacga cgaataatgg attttccaca aagggttaat
     2281 ggttgggcgc tatatgctca tccctgtttt caggaaacct acgacgcttt agttgccgaa
     2341 gtcgagacat taaagggaaa agatcctgaa aattatcaga gaaaagctgc cacaaagtta
     2401 ttggcggtgg tccataaagt gattgaggag catatcacgg tcaatccatc atcaccggca
     2461 ttccgtcatg gcaagtcgtt aggctctggg aaaaataaag actggtcacg ggtaaaattt
     2521 ggtgctggtc gctatcgtct cttctttcgt tacagtgaaa aagagaaagt catcattctg
     2581 ggatggatga acgatgaaaa cacgctgcgc acctacggta aaaaaacaga tgcctatacc
     2641 gtattcagca aaatgttaaa aagaggacat cctcctgccg actgggaaac cctcacccga
     2701 gagacagaag aaacccattg atggtgttca caatgcccgc cgaacgcatt cgtgttgacc
     2761 agcgggcacc ctttctcact ccccgaccag aatcacttca accccagcct ttcgcagccc
     2821 ttccaggcta tccgcaggaa tgccttcatc aacaatgatc atgtcgatac gttgagtatc
     2881 aatgatctta tgtaaactgg aacgattgaa tttactggaa tcggtgacca cgatgatccg
     2941 ttccgcaact tcgcacatcc ggcggtttaa acgggcttca tcttcattat gcgtgctgac
     3001 gccgcgctcc agatcgatcg catctacacc aagaaacagc atatcgaagt ggtaattttg
     3061 cagcgattgc tcagcctgat cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc
     3121 ggtcatcagc agctcaacgc cttccgcttc cagcaacgca ttagccacgt tcataccgtt
     3181 ggtcatcgca attacgtcag tgtgcttgcg catcagacga gcaatctcaa aagtggtggt
     3241 cccggaatcg aggatcaccc gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac
     3301 gctgcgtttc attgcggtgt tcagcgcgct tttatcttcc actgatggct cgactgacgg
     3361 cgttgtgcta tcgcagatca acgcgccacc ataggcacgc acagcgatcc cctgcttttc
     3421 cagaaacgcc agatcgttgc ggatcgtcac ggtagatacg ccatacaatg ccgacagatc
     3481 gttaacctgc acactccctt gctgtcgcag acgctgaatg atctgttctc gtcgctcgct
     3541 ggtgcctgtc actcgcttct cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt
     3601 aaaactttcg tttcatttcg ttttgcctat taacgccttt ctattaagca aatgcaagcc
     3661 caccttgccc attgacgcaa gctactctcg tttcagtgac tttcattatg tttcttttgt
     3721 gaatcagatc agaaaactat tatctttcgt tttattttta tctcaccatg acgcagtatc
     3781 aactgaaaca aaacgaaaga ttaatatcgc agtaatctga actggagagg aaagtgaaac
     3841 atctgacaga aatggtgaga cagcacaaag cgggcaaaac aaatggaatt tatgccgttt
     3901 gttccgcaca tccgctggtg ctggaggctg caatccgcta cgccagtgca aaccaaacgc
     3961 cgttactgat tgaagcaacc tccaatcagg tagaccagtt cggcggttat accggaatga
     4021 cgcccgccga ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg
     4081 atgcgttgat tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac ctgccggccg
     4141 ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta cgttgcggca ggattcaaaa
     4201 aaatccacct tgattgcagc atgtcctgtc aggacgatcc gattccctta actgatgaca
     4261 tcgtggctga acgcgccgcc cgtctggcga aagtggcgga agaaacctgt cttgaacact
     4321 ttggcgaagc cgatctggag tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc
     4381 atgaaacctt aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc acgctggaag
     4441 cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc atcattgccc
     4501 tggtggttca acccggcgtc gaattcgatc acaccaacgt tattgattat cagcccgcca
     4561 aagcgaccgc cttaagccag atggtcgaaa gctacgaaac gctgattttc gaagcgcact
     4621 ctaccgatta tcaaacgccg caatcgctgc gccagctggt gattgaccac tttgccattc
     4681 tgaaagttgg cccggcgctg accttcgccc tgcgtgaagc tctgttctct ctggcagcga
     4741 ttgaagaaga actggtgcca gcaaaagcct gttctggtct gcgtcaggtg ctggaaaacg
     4801 tgatgctcaa ccgcccggaa tactggcaaa gccactacca cggtgacggc aacgcgcgtc
     4861 gtctggcgcg tggttatagc tactcggatc gcgtgcgcta ttactggccg gacagccaga
     4921 ttgatgacgc tttcgctcat ctggtacgta atctggcgga ttcaccaatt ccgctgccgc
     4981 tgatcagcca gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag ctgcagccaa
     5041 cgccacggga actcattatc aaccatattc aggacatcct ggcgcagtac cacacagcct
     5101 gtgaaggcca ataagcaaaa caaagaggaa cacgctatgc caaatattgt tttaagccgg
     5161 attgatgaac gcttgattca cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca
     5221 aatctggtgc tggtcgccaa cgatgaggtt gccgaagatc cggtacaaca aaacctgatg
     5281 gaaatggtac tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa agttatcgac
     5341 aacattcatc gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac acccgccgat
     5401 ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg caatatgcac
     5461 tacgccaatg gcaagcaaca aatcgccaaa acggtttctg tggatgcggg cgatatcgca
     5521 gcatttaacg acctgaaagc cgctggggtg gaatgcttcg ttcagggcat cccgacagag
     5581 cctgctgtgg acctctttaa attactttga gggattcatc atggaaatca gtctgttgca
     5641 ggcattcgcg ttgggcatta tcgcctttat cgctggcctg gatatgttta acggcttaac
     5701 ccatatgcac cgcccagtgg tcctcggccc gttagtcggg ctggtacttg gcgatctgca
     5761 taccggtatt ttaaccggcg gtacgctgga actggtgtgg atggggctgg ccccgctggc
     5821 gggcgcacag ccgcctaatg tgattatcgg tactatcgtc ggcacggcgt ttgccattac
     5881 tactggcgtg aaacccgatg tcgcagtcgg tgtcgccgta cctttcgctg tcgcagtaca
     5941 gatggggatt accttcctgt tctcggtgat gtctggcgtg atgtctcgct gcgaccggat
     6001 ggcggagaac gccgatactc gcggcattga acgcgtaaac tatctggcac tgctggcact
     6061 cggcactttc tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa
     6121 aaccatcatt gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat
     6181 catgccagca atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc
     6241 ctacttcatc ctgggctttg ttgccgcagc ctggctcaag ttaccggtgc tggctattgc
     6301 tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg aaccgactca
     6361 acctgcggca cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg
     6421 tcagtgaaaa cgaagaaaca ttgctgactg gcgtcaatga aaacgtgtat gaagatcaga
     6481 gcattggcgc ggagctgacg aaaaaagata tcaatcgcgt cgcctggcgt tccatgctgt
     6541 tacaagcgtc ttttaactac gaacgtatgc aggcttccgg ctggttgtac ggtctgctgc
     6601 ctgctctgaa aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata
     6661 tgggtttctt caatacccat ccgtttctgg tgacatttgt tatcggcatt atccttgcga
     6721 tggagcgttc taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc
     6781 cgctcggcgg gattggcgat gcaatgttct ggctaacgct actgccgatt tgtggcggga
     6841 ttggagccag tctggcattg caaggctcca ttcttggcgc agtcgtcttt attgtgctgt
     6901 tcaacgtggt ccacctgggg ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg
     6961 tggcggcgat tccactcatt aaagctaata ccaaaaaagt cggccatgct gcgtctatcg
     7021 ttgggatgac ggtaatcggt gcgctggtgg caacctatgt tcgtttaagc accacgctgg
     7081 aaatcacagc gggcgacgca gtggttaagt tacaggctga tgttatcgac aaactgatgc
     7141 ctgccttctt accgttggtc tacaccctga ccatgttttg gttggtacgc cgcggctgga
     7201 gtccgctgcg tctgattgca gttaccgtgg ttctcggcat cgttggtaaa ttctgccatt
     7261 tcctttaaat acaatgaggt attagatgtt aagtattatt ttgacagggc atggcgggtt
     7321 tgccagcggc atggaaaaag caatgaagca aattctcggt gagcaatcgc agtttatcgc
     7381 catcgatttt ccggaaacct caagcaccgc gctgcttaca tcgcagcttg aagaggccat
     7441 cgcccaactg gattgtgaag acggcattgt ttttctgacg gatttgctgg gcggcacgcc
     7501 gtttcgcgta gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga tcaccggcac
     7561 caatttgcaa ctgctactgg agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt
     7621 ccgtgtacag gcgctggagt gtggacatcg tgggctgacc agtctggtgg acgagttagg
     7681 ccgctgccat gaagaatgtc cggtcgagga agggatatga cacacgttct gcgcgccaga
     7741 aggctgctga ctgaagaggg atggctcgat gaccatcagt tgcgtattgc tgacggtgtc
     7801 atcgcagcaa tcgaaccgat tccagtgagc gtgactgaac gcgatgcgga actgctctgc
     7861 cccgcttaca tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg
     7921 ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggttg
     7981 ccgactaccg taaccgcgcc gcttagtacc attcatgcgg cgctgaaacg tattgctcaa
     8041 cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga gttatctcga aggaccgtac
     8101 ttcacgccgc agaataaagg cgcgcatccg ccggagttgt ttcgcgagct tgaaattgcc
     8161 gagctggatc aattgattgc cgtttctcag cacaccttac gcgtggtagc gctggcaccg
     8221 gaaaaagagg gggcattgca ggccatccgc catcttaaac agcaaaatgt acgagtgatg
     8281 ctggggcata gcgcggcgac ctggcaacaa actcgcgccg cgtttgatgc tggtgccgac
     8341 ggcctggtgc attgctataa cgggatgaca ggtttacatc accgcgaacc gggaatggtt
     8401 ggcgcgggat taacggacaa gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg
     8461 catccggcgg caatgtcgct gtgttgttgc tgtgcgaaag agagaatcgt actgatcacc
     8521 gacgcgatgc aggcagccgg gatgccggat ggtcgctata cgttatgtgg cgaagaagtg
     8581 cagatgcacg gtggcgttgt ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct
     8641 gttgatgcgg cagtgcgcaa catggtcgag ttgacgggcg taacgcctgc ggaagccatt
     8701 catatggcgt cgctgcatcc ggcgcgaatg ctgggtgttg atggtgttct gggatcgctt
     8761 aaaccgggca aacgcgccag cgtcgttgcg ctggatagcg ggctgcatgt gcaacaaatc
     8821 tggattcagg gtcaattagc ttcgttttga tggtttgctc cattattggg ccttcacttc
     8881 ccccgtaagg cctttctttt tctttcgttt tgatctgtgc agcggtgtcg gatgcgacgc
     8941 taacgcgtct tatccgacct acagttggtg accgcaaggc cggataaagc gtttgcgccg
     9001 catccagcaa tcccttttgc ttcctttatc ttttctttca acgatcacgg atttcgtttt
     9061 atttcctttt tctccattga actttcggtt tcttttctat agattttaat caacgaaaga
     9121 catcaccaag tgaaatgaaa cgaaaggcaa gtgaaagcga caacgcccga cgtcaagttc
     9181 atcagactaa ggattgagtt atgccagaaa attacacccc tgctgccgcc gcaaccggta
     9241 catggactga agaagagatc cgccatcagc ctcgcgcatg gatccgttcg ctcaccaaca
     9301 tcgacgcgct acattccgcg cttaataact tccttgaacc gttactgcgc aaagagaatc
     9361 tgcggatcat cctgaccgga gccggaacct cggcatttat cggtgacatc atcgcgccgt
     9421 ggctcgccag ccataccggt aaaaacttca gcgccgtacc gaccaccgat ctggtcacta
     9481 atccgatgga ctacctgaac ccagcccatc cgctgctgtt gatctccttt ggtcgatccg
     9541 gcaacagccc ggaaagcgtc gcagccgtgg aactggcaaa tcaatttgta ccggaatgct
     9601 atcacctgcc gatcacctgc aacgaagcgg gcgctcttta ccaaaacgcg atcaacagcg
     9661 ataacgcgtt tgccgtgctg atgcccgcag aaacgcacga tcgcggcttt gcgatgacca
     9721 gcagcattac caccatgatg gccagctgcc tcgcggtttt cgcacctgag acgatcaaca
     9781 gccaaacctt ccgcgacgtg gcggatcgtt gccaggcgat cctgacctca ctgggcgatt
     9841 tcagcgaagg tgtgtttggt tacgcaccgt ggaaacggat cgtttatctc ggcagcggtg
     9901 gcttacaggg cgcagcacgc gagtcggcgc tgaaagtgct ggaactgacg gcgggtaaac
     9961 tggcggcctt ttatgattct ccaaccggat tccgtcatgg accaaaatcg ctggtcgata
    10021 acgaaacact ggtggtggta tttgtctcca gccaccctta cacccgtcag tatgatcttg
    10081 atctgctggc tgaacttcac cgtgacaacc aggcaatgcg tgtaatcgcc atcgccgcgg
    10141 aaagcagcga catcgtcgct gccggtccac atatcatcct gccaccgtca cgtcacttta
    10201 tcgacgttga gcaggcattt tgcttcctga tgtacgccca gacgtttgca ctgatgcagt
    10261 cgctgcacat gggcaatacg ccggataccc catcagccag tggcaccgtt aaccgcgtgg
    10321 tgcaaggcgt aatcattcat ccgtggcagg cataagagga tcgcattatg agcattatct
    10381 ccaccaaata tctgttacag gacgcccagg ccaatggcta cgcggtgcct gcttttaaca
    10441 ttcataacgc cgagacgatc caggcgatcc tcgaagtgtg cagtgaaatg cgatcgccgg
    10501 tgatcctcgc cggaacgccg gggaccttta aacatatcgc gctggaagag atctacgccc
    10561 tgtgcagcgc ctattccaca acctacaaca tgccactggc gctgcatctc gaccaccacg
    10621 aatcgctgga tgatattcgc cgtaaagtcc acgcaggtgt gcgcagtgcg atgatcgacg
    10681 gcagccactt cccgtttgcc gagaacgtaa agctggtgaa atcggttgtc gacttctgcc
    10741 actcccagga ttgcagcgtg gaagcagaac tgggccgcct gggcggtgtt gaagatgaca
    10801 tgagcgttga cgccgaaagt gcattcctga ccgatccaca agaagctaaa cgctttgtcg
    10861 aactgactgg cgtcgacagc ttggcggtag cgattggtac ggcgcacggc ttatacagca
    10921 aaacgccgaa gattgatttc cagcggctgg cggaaattcg tgaagtggtg gatgttcctc
    10981 tggtgctaca tggtgccagc gatgttccgg atgaatttgt ccgtcgcact attgaacttg
    11041 gcgtcacaaa agtgaacgtt gccacagaat taaaaatagc cttcgccggc gcggttaaag
    11101 cctggtttgc ggaaaatccg cagggtaatg atcctcgtta ttatatgcgc gtcggaatgg
    11161 atgcgatgaa agaagttgtc agaaataaaa ttaatgtctg tggtt
//
LOCUS       DAZFKY010000157        10932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFKY010000157 DAZFKY010000000
VERSION     DAZFKY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..66
                     /locus_tag="ACHKCL_003681"
     CDS             <1..66
                     /locus_tag="ACHKCL_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5764565.1"
                     /translation="RLITMQKQLEGTQKYINEQCR"
     gene            complement(98..391)
                     /gene="bor"
                     /locus_tag="ACHKCL_003682"
     CDS             complement(98..391)
                     /gene="bor"
                     /locus_tag="ACHKCL_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO5764566.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            800..1606
                     /locus_tag="ACHKCL_003683"
     CDS             800..1606
                     /locus_tag="ACHKCL_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5764567.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            1587..3293
                     /locus_tag="ACHKCL_003684"
     CDS             1587..3293
                     /locus_tag="ACHKCL_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5764568.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            3293..5437
                     /locus_tag="ACHKCL_003685"
     CDS             3293..5437
                     /locus_tag="ACHKCL_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5764569.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            5595..6602
                     /locus_tag="ACHKCL_003686"
     CDS             5595..6602
                     /locus_tag="ACHKCL_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764570.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            6626..7840
                     /locus_tag="ACHKCL_003687"
     CDS             6626..7840
                     /locus_tag="ACHKCL_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO5764571.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            7896..8285
                     /locus_tag="ACHKCL_003688"
     CDS             7896..8285
                     /locus_tag="ACHKCL_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764572.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            8335..8796
                     /locus_tag="ACHKCL_003689"
     CDS             8335..8796
                     /locus_tag="ACHKCL_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764573.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            8780..9343
                     /locus_tag="ACHKCL_003690"
     CDS             8780..9343
                     /locus_tag="ACHKCL_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764574.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            9343..9993
                     /locus_tag="ACHKCL_003691"
     CDS             9343..9993
                     /locus_tag="ACHKCL_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO5764575.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALYARKKVFNGGGV"
     gene            9990..>10932
                     /locus_tag="ACHKCL_003692"
     CDS             9990..>10932
                     /locus_tag="ACHKCL_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO5764576.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         2832 a         2514 c         3111 g         2475 t
ORIGIN      
        1 aggctgatca ctatgcaaaa acaactggaa ggaacccaga agtatattaa tgagcagtgc
       61 agatagagtt gcccatatcg atgggcaact catgcaatta ttgtgagcaa tacacccgcg
      121 cttccagcgg agtataaatg cctaaagtaa taaaaccgag caatccattt acgaatgttt
      181 gctgggtttc tgttttaaca acattttctg tgccgccaca aattttggct gcatcgacag
      241 ttttcttctg cccaattcca gaaacgaaga aatgatgggt gatggtttcc tttggtgcta
      301 ctgctgtctg tttgttttga acagtaaacg tctgttgagc acatcctgta ataagcaggg
      361 ccagcgcagt agcgagtagc atttttttca tggtgttatt cccgatgctt tttgaagttc
      421 gcagaatcgt atgtgtagaa aattaaacaa accctaaaca atgagttgaa atttcatatt
      481 gttaatattt attaatgtat gtcaggtgcg atgaatcgtc attgtattcc cggattaact
      541 atgtccacag ccctgacggg gaacttctct gcgggagtgt gcggggataa tcaaaaacga
      601 tgcacaccgg gttttctcat ttttcacgag atgggagcga tttcccgcga agccgcctgt
      661 ccggtgcggt ggtggaagaa accggataaa acaaccgcat tgtgcaaata tcgatcaaat
      721 atggtgctgc tgtgtgaaat ctgaaaaatc acagcggtca ttatgcatca gtttttaaca
      781 caggacgtca gaacgtgaca tggcaaagct ggactggaaa aagctggagc aggcattccg
      841 acgcgaacat gccgaaacgg gaataacatt actggactgg tgccggaaga aaaagattaa
      901 ttacaacacc gccagaaccc gtataaaaat gggcaaaatc gatcatgaaa ttgatcataa
      961 aaccgatcat gaaatcgatc atgacatctc agatgaagaa ccctgcaatg acgcgggttc
     1021 cggcgatgaa aaatgtgcaa aaaactctga aaaaaactgc gcaaattcgg cagaaacgaa
     1081 acggattcgt ggttcccgac ttttaccacc ttcaaacgct ttttctcagc gaaacaccca
     1141 cgccgtaaga caccgtggat atgcgaagta tcttgaggca gataacctca tggatgatgc
     1201 gtccgacatg gtgctgttcg atgaactggt gttcacccgg gcccgcgcac tttcagtaac
     1261 taaggcactt aaagggatgt tcgccgacct ggaagaggca actgacgtgg aaacccgtgt
     1321 tgctctttac gacaaaatac tcaaagctga acaggccctt gaccggaata ttgcccgtat
     1381 cgagtcaatt gaacgctcat tgctgacgct ggacgtcctg gctgagacag caccaaaact
     1441 tcgtgctgac cgggaaagaa tcaacgccgc cagagataaa ctcagagctg aaaccgatat
     1501 tctgaccaac cagcgtcggg gcgttgttac gcctgtcagt gacatcgtgt catcgctgca
     1561 tgaaatgagt aattcgggga gactggatga cattccggaa gaatgaaccg cgatgtgatg
     1621 agccgtcaga aatgaccgag gctgaacaac gtctgttcat catgactaaa ctgagcaatc
     1681 cctggtggcg gctcaatcat ctctacaaaa tacagaacga aaaaggtgaa ctggtcacct
     1741 tcagaatgcg accggcgcag cgccagttgt tccggagcat gcacaataaa aatattatcc
     1801 tgaaagcgcg ccagctggga ttttccacag ccattgatat ttatcttctc gaccaggcat
     1861 tattcattcc gcatctcaaa tgcgggatcg tcgctcagga taaacaggct gccagtgaaa
     1921 ttttccgcac aaaaattgct gtaccgtttg atcatctccc tgactggctg agagcctcat
     1981 tcaccatcgt tgaacgtcgt agcggtgcca gcggtggcta tatcctgttt ggtcacggct
     2041 cgagtatcca ggtggcaacc tcattccgtt caggtacggt gcagcgcctg catatctcag
     2101 agcacggcaa aatttgcgcg aaatatccgg ctaaggcgaa agaactgcga accggtacgc
     2161 ttaatgccgt ctctgatgaa tgcattattt ttgatgagtc cactgctgaa ggcgtgggtg
     2221 gtgattttta cgagatgagt aaccgagcac aggagatcac tgcatcaggc ttattgctga
     2281 cggcacagga ttataaattc catttttacg cctggtggca ggatcctaaa tacagcgcca
     2341 gagtgccgga aagcgggctg aagctgtcac gggaaaaaat gacgtatttt tctgcggttg
     2401 agaaggcaat gaacatcacg cttactgatg aacagaagca gtggtacatc aataaggaaa
     2461 ctgaacagcg tgaggaaatg aagcaggagt ttccctcaac gccacaggag gcgtttctga
     2521 cgtccggacg acgtgtgttc agtgccgaaa gtacgttgca ggcagaatca ttctgttcgc
     2581 caccgatgat tgtttatgac attgaacctg ttacaggagc gaagactaaa gctcagtctc
     2641 tgcgtgaagg aaataaaaac gagttgcagc ggacgctgat gaattatctg ctggtatggg
     2701 aactgccgga tccggatgaa gagtatgttt gtggggcaga tactgccgaa gggctggagc
     2761 acggagaccg ctcatcgctg gatgttgtca aacgcagtaa tggcgagcag gtggctcact
     2821 ggttcgggca tctcgatgct gaactttttg ctcatctcat ttcgcaggtc tgtcgtatgt
     2881 ataacaacgc gtttgtgggg ccggagcgta ataatcacgg acatgcagtt atcctgaaac
     2941 tccgggaact ctatccgaca cgttatatct acaacgaaca gcatcttgac caggcatatg
     3001 acgacgatac gccccgcctt ggctggctga caacccgtca gagcaaacct gttctgaccg
     3061 aaggaatgaa aacgcttctg aataatggaa tatcagggat ccgctggtca ggcacattat
     3121 cggaaatgaa cacctacgtt tatgacgcga aaggctccat gaatgcacag gaaggctgct
     3181 ttgatgatca gctcatgagc tacatgattg cccaggagat gcgcgccaga atgccggtga
     3241 gggtaaaaca gaaaacggat aaacgcagaa ccacacactg gatggctcac tgatgaaaaa
     3301 tgaaactaac accatggcga cgaaaaacga caatggagcc acgccgcgtt tttctcagcg
     3361 ccagttacag gcgctttgtt ctgatattga cagccagcct aaatggcgtg atgccgcaaa
     3421 caaggcctgt gcgtattacg atggcgatca gttgccaccg gaagttcttc aggtactgaa
     3481 agatcgcggt cagccgatga ctatccataa cctcatcgcg cctaccgtcg atggcgttct
     3541 gggaatggag gccaaaacac ggactgatct ggtggtgatg tcagacgagc cagatgatga
     3601 aactgaaaaa ctggctgaag ctattaatgc tgaatttgcc gatgcatgcc gccttggcaa
     3661 tatgaataaa gcccgctctg atgcctatgc ggaacaaatc aaggcgggcc tcagttgggt
     3721 ggaggtcaga cgaaacagcg atccgttcgg gcctgaattt aaggtgtcta ctgtcagccg
     3781 gaatgaggtt ttctgggact ggctgagccg ggaggctgat ttaagtgact gccgatggct
     3841 gatgcgtcgc cgctggatgg ataccgatga ggcaaaagct acattcccgg gaatggctca
     3901 ggttatcgat tatgccattg atgactggcg tggttttgtc gataccacgg ttactgaagg
     3961 ccagcccagt ccgttgatga gtgcatggga agagtatcag tcatgggatc gacagcagaa
     4021 cgaatggctt cagcgtgaac gccgtcgtgt gctgcttcag gtggtttatt accgtacatt
     4081 cgagcgtctt ccggtgattg aactcagtaa tggacgggtg gtggcctttg ataaaaataa
     4141 tctgatgcag gcggtagctg tggcatccgg gcgggttcag gtgaaagtcg ggcgggtaag
     4201 ccgtattcgt gaagcctggt ttgtcgggcc acactttatt gtggatcgcc cctgtagtgc
     4261 tccgcagggg atgtttccgc tggttccttt ctggggatac cgaaaggata aaaccgggga
     4321 gccatacggg ctaatttccc gcgccattcc ggcacaggat gaggtgaatt ttcgtcgtat
     4381 caagctgacc tggttgcttc aggccaaacg cgtgattatg gacgaggatg ccacccagtt
     4441 gtcagacaac gacctgatgg agcagatcga acgtccggat ggcattatta aactgaatcc
     4501 ggtccgaaaa aatcagaaaa gtgtcgcaga tgtttttcgg gttgagcagg attttcaggt
     4561 tgccagccag cagtttcagg tcatgcagga atcggaaaaa cttatccagg ataccatggg
     4621 agtgtattcc gcatttctcg ggcaggattc aggtgcgacg tcaggcgtgg ctatcagtaa
     4681 cctggtggag cagggggcca caacccttgc ggaaatcaac gataactacc agtttgcctg
     4741 ccagcaggtg ggaagactgt tgctggctta tcttctcgat gacctgaaaa aacgccgtaa
     4801 tcatgcagtg gtgattaatc gcgatgatcg ccagcgtcgc cagaccattg tcctcaatgc
     4861 tgaaggtgat aatggtgaac tgaccaatga tatttcaagg ttaaatacac atattgcgct
     4921 ggcgcctgtt cagcagacac cggcgtttaa ggcacagctt gcacagagaa tgtcagaggt
     4981 tattcagggg ctgccgcctc aggtgcaggc tgttgtgctc gacctgtggg ttaatcttct
     5041 ggatgtgccg cagaaacagg agtttgttga gcgtattcgt gctgcgctgg ggacgccaaa
     5101 atcaccggat gaaatgacgc cggaagaaca ggaagtagcg gcacaacaac aggcacttca
     5161 gcaacaacag gcagaactcc agatgcgcga gatggctggc agagtggcaa aactggaagc
     5221 tgacgccgcc agggcacatg cagctgcaca acgggataat gccagtgcac agcgggaagt
     5281 cgccctgaca caggggcagc gttatgtgga tgcgcttaac caggcacata cggcagaaat
     5341 cattaccggc gtacagaata tggaacagga gcaggacgtt cttcagcaac agatgctgta
     5401 tacgttacaa cagcggatga atgaaatgtc gctctgaaaa ctctggcttc aactgaaccc
     5461 cgtcatcgta cggggttttt tgtttccgga ggtaagcgtt ccgggagcgg tgcgcttatt
     5521 cgcgggggca gcgataagcc ttatttactc aaccattcgg atctgtccga taaacagacc
     5581 atgcggagtt atttatggat tttgaattta cgggtgaaga aaccccggaa caactggaaa
     5641 aaatgctgga aggacttggg gatgtggata ttgacagtca cgcacaggac gtcgtgacgg
     5701 aagataccac ggaaaaacat gcggatgagg aagcacagac tcagacgggc gataacaatg
     5761 tggcaccgac gccggatgcc agtgtggagc agacgcagga cgtgaaggag ccggaagcga
     5821 agggggtgct cacccgcgac ggtaaacacg tcattcccta tgaagtcctt gaggctgaac
     5881 gttccggtaa gcaacgggcc gaacaggaag ccgcacttct tcgtgggcag atagctgaag
     5941 aaaaacgcag ggtggaactg ctgacgtctc agatccacca ggccggtatg aagcccacac
     6001 cgttaccgga aaacgaaaaa atttctgatg agcagattgc ccgtatcagg gagatgtatc
     6061 cggaaattgg tgacgcggtg gcttcgctca tccgtaaaaa taactatctc cagtcccgtg
     6121 ttcagcaatc agcacagcag gcagaaggta atggtggtga ggatttatca ccggttcttg
     6181 atgcgatgaa tgccgtgccg gtgctgaaaa cgtggcagga gtccgatcca gatcgcttct
     6241 cggttgctgt atccatcgac gggaagctcc agaatgaccc cgcatggaaa gacaaaacgc
     6301 tcactgaacg tttcgctgaa gtggcccgtc gtacgcaggt tgctttcggt gaagtcagtg
     6361 agtcgtctgc tgacaacaag gcagacaaaa cggatatccg gaaaacggcg gaagagaaag
     6421 tgaagaccgc tgaacaggag caggcagtac ctgcttcccc gtcagattta ggcaccacgg
     6481 cttccgtcgg aaccggtgat aattttgaac ggttacttgg cgcttctcat tcagaggcag
     6541 aggcgattat gcgcggtatg acgaatgctg aaatagacgc gcttctggag aagctcgggt
     6601 aacttactga aggagaactg aagtaatgac gactgtaaca tcagcccagg cgaataagct
     6661 gtatcaggtg gcgcttttta ccgctgccaa ccgcaaccgc tcgatggtaa atatcctcac
     6721 tgaacagcag gaagcgccaa aagcggtttc gccggacaag aaaagcacga agcagaccag
     6781 cgcgggtgcg ccggttgtcc gtatcacaga ccttaacaaa caggccggtg atgaagtgac
     6841 cttcagcatc atgcacaaac tctcaaaacg tccgacgatg ggagatgagc gtgttgaagg
     6901 tcgtggtgag gatctcagcc atgctgactt ctccctgaaa atcaatcagg gacgtcacct
     6961 ggtggatgca ggcggacgta tgagtcagca gcgcacgaag tttaacctgg catcctcagc
     7021 cagaacgctt ctggggacgt actttaatga cctgcaggac cagtgtgcga tagtgcatct
     7081 tgctggagct cgtggtgatt ttgttgctga cgacactatt ctgccgacag cggagcaccc
     7141 tgaattcaaa aaaatcatga tcaacgatgt actgcctccg acacatgacc gtcacttttt
     7201 tggcggtgat gcgacaagct ttgagcagat tgaagcggca gatatttttt ctattggcct
     7261 ggtggacaat ctctccctgt tcattgacga aatggcgcat ccgttacagc cggttcgtct
     7321 gtccggtgat gaacttcacg gagaagatcc atattacgtc ctgtacgtca cgccgcgtca
     7381 gtggaatgac tggtacacct cgacgtccgg taaggactgg aaccagatga tggttcgtgc
     7441 cgtgaaccgt gcaaaaggtt ttaatcatcc gctgttcaaa ggtgaatgtg cgatgtggcg
     7501 caatatcctg gttcgtaagt atgcgggtat gccgatccgt ttctatcagg ggtcaaaggt
     7561 tctggtatca gagaataacc tgacggcaac cacgaaagag gtcgctgctg caaccaatat
     7621 tgaccgcgcc atgttactgg gggctcaggc gctggcaaat gcttacggtc agaaggcggg
     7681 cggtcacttc aacatggttg agaagaaaac ggatatggat aaccgtactg agatagcaat
     7741 cagctggatc aacggtctga aaaaaatccg tttccccgag aagagcggca agatgcagga
     7801 tcacggcgtg attgccgttg atacagcagt gaagctctga ttttttcctt tccctatgcc
     7861 gggttttcgc ccggcttttt caggagtcat taattatggc aaagactatc cttgccccgt
     7921 cactgagtga acgggtctat acgggtacgc acggtaatga gtcggtggca gaaggcgtat
     7981 ttacggtgaa tgctgcggaa gcggacagtg ttattcatct tctctcactg ccagtgggca
     8041 tccgtatcaa ctcactccaa ctggtttcaa cgggtggtct gggtactgca accgtcagca
     8101 ttaagtccgg tgagcatgct ctcatcgata acagcgaagc tgtttctgca aaatttgcca
     8161 gatatgtgcc agtggagccg tacaccacac agcgtgacgg ggagctggtt actgtcacca
     8221 ttaagactgc cgctgcaacc ggcaccctga atgttctgct gcgttatacc gtggtgggat
     8281 actgattaaa accttccggc ccgcgtcatg cgggcttttt atccggggaa ttatatgagt
     8341 gagaaaattg ccgttgtcta tatcggccca aaacccgtga aaaaggacac cattaccgga
     8401 agtcgcacgc tgttcccacg tcttgagccg gtgcatgttg acagcgcgat ggcctggcaa
     8461 ctgctggggt ttccggatgt ctgggttcgt catgaagagc ttgatgatgt tctgaaaaag
     8521 caacaacaga atgagcagtt gcggcaggca cagcaggcgc aggaaagagt gcttgctgcg
     8581 ctggcagaag cggagaacag ttttgttgtt tctgttaacg ggcaggaggt ggatttaagt
     8641 aagctcacct cagcacggct ggcgacgctg tgtgaggcag aagagctgga tattcacaaa
     8701 gacccgaaag aaacggctga ggcattccgt atccgggtgc gtgaggcatt tcgccgtcgt
     8761 gttgcggaga ctgaacagca tggcggaact gagtgatttt ttaccgtatg tccgtcgtca
     8821 tatcagcggt ccactgaaca ttatgatgac ggatgctctg tcaatggctg ccgtggcatt
     8881 cagccgccag tcgttggtgt gccgtcggga ggttactgtt gtaccagtag caggaaaaga
     8941 aatcgtgctt ccgtatgaca aagatgatga ggagtgcgtt catatcatcc gtatctctga
     9001 cgataatcat gagctttttg tcggtcggga tgtggatatc agctccggac gctccctgcg
     9061 atttgcctgt tctcccggtg aggtgagcgt gctttatgcc gtcgctccga aagccggacg
     9121 cagccagata ccggatgaac tcctcacatg gcctgaagaa gtggctgcgg gggcacttga
     9181 gcggttgttc atgcagactg gtgtttcatg gtcagatccg ttacgcgcac agtatttttc
     9241 tgtgcagttt tctgagggga tccgtcgggc atatcgtcat acactggcga caagcccgta
     9301 ctcttcatac cgcaaccctg tacgcaggca gaggtttttc tgatgacgac gattacagaa
     9361 atcatcggac gtgtgaatac acaactggtt gacccgatga tggttcgctg gcccctgcag
     9421 gaattgtgcg attattacaa tgatgctgtg agggcagtga ttctggcgag accggatgct
     9481 ggcgcaagcc tggaaacaat aagttgtgtt cctggcgccc gtcaggtttt gcccgatggt
     9541 gtaatacaac ttcttgacgt gatatgcctc agtgacggta gtgcagtcag accattatcc
     9601 cgggaggtgc tggatgcgca gtatcctgag tggcccacaa tgaagggtat tcctgaatgt
     9661 tttatcagca acgacctgtc cccgcgcgta ttctggctgt ttcctgctcc tgacaaagag
     9721 ataagtattg atgcagtggt aagccggata ccggaggcag tgtatgttct gacgcaggac
     9781 gatgatacgc cagttccact ggaagaggct tatgttaacc cactggtgga gtggatgttg
     9841 tttcgcgctt tcagtaagga tgctgccggt ggcgcagaat cggggctggc tgcgcagcat
     9901 tatcagagtt ttgttgagca acttgggatc aaacaggggg cagacagtgc attgtatgcc
     9961 cgtaaaaaag tgtttaacgg aggtggagtg tgagtgttgt tgtttcgggg acgctgaaat
    10021 ctcctgatgg tgaggcgata tcaggagcaa atattaccct gacggcgctg acagtttcac
    10081 cggatgcgct cagcggcacc agtgcgtcgg cagtgacccg tgaaggtgga tattacggaa
    10141 tgacgatgga tccgggggag tatgcggttt cggtgacggt gaaagggaag actgctgtct
    10201 acggacgtgt gcgtattgag gggaccgaaa gtacggtgac gctcaatatg ctgttacgcc
    10261 gcagtcttgt tgaggttagc atacccggag aactgctgac agatttccgg cagatacaga
    10321 ataatgtggc tgatgacctt gccactattc gtcgcctgaa tgaagacacg gcgacaaaaa
    10381 acactcaggc cacacagtca aaagaaagtg cagcagccag tgcgaagagt gcatctgaca
    10441 gtgcaaagac ggcaaccagc agggcggctg aagccggaca aaaagcgact gatgccactg
    10501 aggctgcgac ccgtgcagtc acagcagcgg ggaatgcaga ggaaagctcg acccgtgccg
    10561 gagagtctga aaaagccgcc ggagctgatg cagaaaaagc cagacagcat gctgaaaagg
    10621 ccaggctggc gcaggagagc gccggagaga tccttaagcg ggcagaggct gccactgtca
    10681 gtgctgaaga ggccagacgt atggctgaga atgcacgggg gccccggggg cctcagggag
    10741 aaactggtcc gaagggggat gtcggtccta aaggcgaaac aggtccagtg ggccctcaag
    10801 ggcccgcagg gccgaaaggt gagcgtggtg acgttggtgc tcagggggct gtagggcctg
    10861 ctggtccgcg tggtgagaag ggcgaacagg gggagcgagg accgcaggga ataccaggcc
    10921 tgaaggggga ta
//
LOCUS       DAZFKY010000158        10930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFKY010000158 DAZFKY010000000
VERSION     DAZFKY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKCL_003693"
     CDS             complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKCL_003693"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5764577.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2611..3519)
                     /gene="melR"
                     /locus_tag="ACHKCL_003694"
     CDS             complement(2611..3519)
                     /gene="melR"
                     /locus_tag="ACHKCL_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5764578.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3802..5157
                     /gene="melA"
                     /locus_tag="ACHKCL_003695"
     CDS             3802..5157
                     /gene="melA"
                     /locus_tag="ACHKCL_003695"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5764579.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5260..6681
                     /gene="melB"
                     /locus_tag="ACHKCL_003696"
     CDS             5260..6681
                     /gene="melB"
                     /locus_tag="ACHKCL_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO5764580.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6820..7449)
                     /gene="yjdF"
                     /locus_tag="ACHKCL_003697"
     CDS             complement(6820..7449)
                     /gene="yjdF"
                     /locus_tag="ACHKCL_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5764581.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7572..9218)
                     /gene="fumB"
                     /locus_tag="ACHKCL_003698"
     CDS             complement(7572..9218)
                     /gene="fumB"
                     /locus_tag="ACHKCL_003698"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5764582.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEKAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(9296..10636)
                     /gene="dcuB"
                     /locus_tag="ACHKCL_003699"
     CDS             complement(9296..10636)
                     /gene="dcuB"
                     /locus_tag="ACHKCL_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5764583.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2556 a         2675 c         2902 g         2797 t
ORIGIN      
        1 aaaaaaaagc gcggggtggg gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt
       61 tatgaaaacg ttgccatact caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca
      121 attacgcctt cagcggaata gtggttacgc tttcacacac ataacgtggt aaataccgtc
      181 aataatttca gtcccttcag tttcgtgttc aaatccaggg aaatgatggt cccaggattg
      241 cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt caccagacag
      301 cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatac gtcctggcag
      361 attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg
      421 ggtgatttcc gccaccggca ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt
      481 ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt
      541 gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt tggcgtcata
      601 gtggcgtttg aaggagcaaa gggtgttaac cagagtcccc catttcccac gggttacgcc
      661 catagagaac aggaacataa tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg
      721 accaagccag gcagtgacca gcgccgccgg aacaccggtt tcttccagtt caccatcttc
      781 acccattccc ggagcaagga tgctgacttt aatcgggtcg agcatactcc agttatccgg
      841 aatatctttg aagccgtgcc agctttcgcc cggatgcatt acccagcagt cctgaacggt
      901 ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt
      961 gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggcga actctttata
     1021 cagccgcgcc atcgcctgac ggaaatcaac cgcttcgtcg atcacttcct gcgtcagtga
     1081 caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc agatggcata
     1141 cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga agttaatcgc
     1201 cccacgacct tcacgtacat gaatataaga ggcctgtgaa agcgcattca gcagtttgtg
     1261 ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc
     1321 atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg
     1381 cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat aacacacgcc
     1441 gtcataggtg cagttggtca ccacgcagta agacggtttt tgcccggctt tgtctttagt
     1501 cagcgggctt tcactgattt tcttctgcaa ggtttcaggt tgcatttcct gcggatagat
     1561 tggcccgata atgccgtagc ggttgcggct tggcaccatg tagaccggtt tcgcgcctgt
     1621 cagcatcaaa ccttgttcga tggatttatg gcagttacgg tcaacgacca cgacatcgtt
     1681 atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga cgactaccga
     1741 ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc
     1801 agtatggtca agcaaagaac cgagggaagt tcgttcgatg cccatgtcgg tgcggaacag
     1861 attttcaccg tagtagtcat ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc
     1921 gccctggtgg cctggcgctg cccaggaata ttcatggatg tcgctatatt tcatcagcgc
     1981 gctgaacagt ggcggcaaca gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc
     2041 ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa gctccagcag
     2101 atcccggtcc attgcggcga gggctttttc ccgatcgccc aacaggaaga ccggcacgtt
     2161 ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg
     2221 ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa
     2281 accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta aagcatctgc
     2341 cagacgctca acagcgttac cgacccaggt gtcttgatgg agaaactcgc tttcaacaat
     2401 taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg caagtatctt
     2461 ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata tttgttttta
     2521 taaacaatat gttatgtgat tattttaaat tattttcact gcttattatc gacggctaaa
     2581 ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc ggcgctgttg
     2641 gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa
     2701 acgactactt gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt tatcggtatc
     2761 gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa
     2821 ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga cgtgctcagc
     2881 cacatcgttg atggtcagcg cctggtcata gttttcggca ataaagccca gcatctggct
     2941 aacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac
     3001 cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa tttcatcaat
     3061 ggcgagctgg cgaatttgct cattcggact gtttaattcc tgctgccagc ggcgcacttc
     3121 aaacgggcta agttgctgtg tagcaagtga tttgatcacc atgccgtgag tgacgtggtt
     3181 aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat taaaaatcgc
     3241 catgctctga caggttccgg tatctgttag ttggtgcggt atacaggccc agaacagcgt
     3301 gatatgtccc tgattgatat tcactttttc attgttgatc aggtattcca catcgccatc
     3361 gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga tatgcggtgc
     3421 gcgaaactca atctccatcc gctggtattc cgaatacagc gacagcgggc tgcgggtctg
     3481 tttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatggctctc tttcctggaa
     3541 tatcagaatt atggcaggag tgagggagga tgactgcgag tgggagcacg gttttcaccc
     3601 tcttcccaga ggggcgaggg gagtgtccga gcatcatgag gccgaaaact ctgcttttca
     3661 ggtaaattat tcccataaac tcagatttac tgctgcttca cgcaggatct gagtttatgg
     3721 gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga tgaagttatt
     3781 caagcaagcc aggagatctg catgatgtct gcacccaaaa ttacatttat cggcgctggt
     3841 tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgcgaggc gctgaaaacg
     3901 gcgcatattg ccctgatgga cattgacccc acccgcctgg aagagtcgca tattgtggtg
     3961 cgtaagctga tggattcagc aggggccagc ggcaaaatca cctgccacac ccaacagaaa
     4021 gaagccttac aggatgccga ttttgtcgtg gtggcatttc agattggcgg ttatgcacct
     4081 tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac cattgccgat
     4141 acgttggggc cgggcggtat tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt
     4201 tgcgaggaca tgacggaagt ctgccccgat gccaccatgc tcaactatgt taacccaatg
     4261 gcgatgaata cctgggcgat gtatgcccgc tatccgcata tcaaacaggt cgggctgtgc
     4321 cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga cccggctacg
     4381 ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt acctggagct ggagcgcaaa
     4441 accgccgacg gcagttacgt gaatctctac ccggaactgc tggcggctta tgacgcaggg
     4501 caggcaccga agccgaatat tcacggcaat actcgctgcc agaatattgt gcgctatgaa
     4561 atgttcaaaa agctgggcta tttcgtcacg gaatcgtcag aacattttgc tgagtacaca
     4621 ccgtggttta ttaagccagg tcgtgaggat ttgattgagc gttataaagt accgctggat
     4681 gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct ggaggagtat
     4741 aaaaacgcct cccggattga tattaaaccg tcacgggaat atgccagcac aatcatgaac
     4801 gctatctgga cgggcgagcc gagtgtgatt tacggcaacg tccgtaacga tggtttgatt
     4861 gataacctgc cacaaggatg ttgcgtggaa gtggcctgtc tggttgatgc taatggcatt
     4921 cagccgacca aagtcggtac gctaccttcg catctggccg ccctgatgca aaccaacatc
     4981 aacgtacaga cgctgctgac cgaagctatt cttacggaaa atcgcgaccg tgtttaccac
     5041 gctgcgatga tggacccgca tactgccgcc gtgctgggca ttgacgaaat atatgctctt
     5101 gttgacgacc tgattgccgc ccacggcgac tggctgccag gctggttgca ccgttaaaac
     5161 gcgactaaac gctactgcgc cgggggattt attccggcgc acacctctga cgataccaat
     5221 aacagaaggc gggcgttggt aacagcgacc cgatacccta tgagcatttc aatgactaca
     5281 aaactcagtt atggatttgg agcgttcggg aaggattttg cgatcggcat tgtgtatatg
     5341 tacctcatgt attactacac cgatgtcgtc gggctgtctg tgggtttggt cggtactttg
     5401 tttctggtgg cgaggatctg ggatgctatt aacgatccga ttatgggatg gattgtaaat
     5461 gctacgcgat cgcgatgggg taagttcaaa ccctggatcc tgatcggtac gttggcaaac
     5521 tctgtaatct tatttctcct ctttagtgcg catctgtttg aaggtactac tcagattgcc
     5581 tttgtttgcg tgacctacat cctctggggc atgacttaca ccattatgga tattcccttc
     5641 tggtcgctgg ttccaaccat cacgctcgat aaacgtgagc gtgaacaact ggttccttat
     5701 ccgcgttttt ttgccagtct ggcgggcttt gttacggcag gtgtgacgct accatttgtt
     5761 aattatgtcg gcggtggcga tcggggattt ggctttcaga tgttcacact ggtactgatc
     5821 gcctttttta ttgtttcaac catcatcact ctgcgcaatg tgcatgaagt cttttcgtca
     5881 gacaatcaac cgtctgctga aggaagccat ctgacactta aagccatcgt tgcgcttatt
     5941 tataaaaacg atcagctttc atgcctcttg ggtatggctc ttgcttataa tgtagccagc
     6001 aacattatta ccggctttgc tatctattat ttctcatatg ttatcggtga tgcggatttg
     6061 ttcccctatt atctgtcgta tgcgggagct gctaacctgg tgacgttagt attcttccca
     6121 cgcttagtta aatcattatc ccgacgcatt ttatgggccg gagcatctat tcttccggtg
     6181 ttaagctgtg gtgttctcct gttaatggca ttaatgagct atcacaacgt cgtcctcatt
     6241 gtgattgcgg gtattttgct gaatgtggga acggcgcttt tctgggtatt acaggtcatc
     6301 atggtggcag ataccgttga ttacggtgaa tataaactgc acgtacgctg tgaaagcatc
     6361 gcttactccg tgcagactat ggtggtgaag ggcggttcag cctttgcggc ttttttcatt
     6421 gcggttgtgt tagggatgat tggctatgta ccgaatgttg aacagtctac gcaagcccta
     6481 ttaggtatgc agtttattat gattgctcta ccgactctgt ttttcatggt aacgctgatt
     6541 ctctacttcc gtttctatcg cctcaatggc gacacgctgc gcaggatcca gattcatctg
     6601 ctggataaat atcgcaaaat accgcccgag cctgttcatg ctgatattcc ggtcggtgca
     6661 gtgagtgatg tgaaagcctg acgtggacaa tagctaacga aaatggcctg atgcgatatg
     6721 tttatcaggc cacgtcttaa tggaatgctt tggatttgca aattttgtag accggataag
     6781 gaattcacgc cgcatccggc atcaacaaag tgcacgttgt tatccggtta tcaagccaaa
     6841 gcgccgtagc tgacggcaat gaaagcgagc gaggaatatc accgtggtta atgcgccaag
     6901 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
     6961 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
     7021 tatcgccagc gccacgcaac agaccagaaa cgccaccatt ttacgtccgc gaacgtacat
     7081 cccgcgcaca aggatctctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
     7141 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
     7201 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
     7261 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
     7321 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
     7381 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gcttgagtgt
     7441 gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcgtgaa
     7501 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
     7561 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgatgatttg ctggaagaag
     7621 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
     7681 atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc
     7741 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
     7801 gcgtcggtaa cctgctgact gcggttacct ttcgctagca tgatcatgct gccgccgtgg
     7861 gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc aagtgagcct
     7921 gatggataac cggcaggggt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     7981 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     8041 cggcccacga taatggtgcc ggtgagcgac aaacgagtgg aaaccggata ttgcgaaagc
     8101 tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc gccttcaccg
     8161 gcctggcgca gttcttgtgg aatgtactga cctggatggt gttccagttt ttcgatccag
     8221 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     8281 cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     8341 tatttaccgc caaactgcgc gccaagaccg agtttctggg ccttttccag cagttcctgt
     8401 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     8461 taatagtgag cgcttgctaa cttaacggtt ttcaggttgg tttccacaga cgtaccgcca
     8521 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     8581 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatag
     8641 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     8701 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     8761 tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt atagacgcct
     8821 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     8881 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     8941 tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     9001 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtggaaggct
     9061 tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     9121 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     9181 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     9241 atttgttatt cgaaaatggc gtgccgcgta gtaacgggcg gcacgcaaag tgcatttata
     9301 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     9361 accccggcag aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     9421 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     9481 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgctagc gcgaccggaa
     9541 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     9601 tggcataggc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     9661 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     9721 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     9781 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     9841 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagtgcg actacagcga
     9901 ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttatccagca
     9961 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
    10021 actcttcgtc tttatccaga tctttaccgc ggaaccagct gaagataccg atcgccagga
    10081 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
    10141 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
    10201 tggcgataat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
    10261 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
    10321 taccgcaaag aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg
    10381 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
    10441 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca actggtggtt
    10501 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
    10561 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
    10621 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
    10681 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
    10741 gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa
    10801 atttatgcac tgtgataagc ggctttttca gagagaaaaa ctctctggcg atgggtttaa
    10861 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
    10921 ctattttttg
//
LOCUS       DAZFKY010000159        10846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFKY010000159 DAZFKY010000000
VERSION     DAZFKY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..803
                     /gene="adiY"
                     /locus_tag="ACHKCL_003700"
     CDS             42..803
                     /gene="adiY"
                     /locus_tag="ACHKCL_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5764584.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            940..2277
                     /gene="adiC"
                     /locus_tag="ACHKCL_003701"
     CDS             940..2277
                     /gene="adiC"
                     /locus_tag="ACHKCL_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5764585.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2381..4024
                     /gene="eptA"
                     /locus_tag="ACHKCL_003702"
     CDS             2381..4024
                     /gene="eptA"
                     /locus_tag="ACHKCL_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5764586.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4021..4689
                     /gene="basR"
                     /locus_tag="ACHKCL_003703"
     CDS             4021..4689
                     /gene="basR"
                     /locus_tag="ACHKCL_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5764587.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4690..5790
                     /gene="pmrB"
                     /locus_tag="ACHKCL_003704"
     CDS             4690..5790
                     /gene="pmrB"
                     /locus_tag="ACHKCL_003704"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5764588.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5766..5855)
                     /gene="pmrR"
                     /locus_tag="ACHKCL_003705"
     CDS             complement(5766..5855)
                     /gene="pmrR"
                     /locus_tag="ACHKCL_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5764589.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5967..7469)
                     /gene="proP"
                     /locus_tag="ACHKCL_003706"
     CDS             complement(5967..7469)
                     /gene="proP"
                     /locus_tag="ACHKCL_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5764590.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7733..8611)
                     /gene="rdcB"
                     /locus_tag="ACHKCL_003707"
     CDS             complement(7733..8611)
                     /gene="rdcB"
                     /locus_tag="ACHKCL_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO5764591.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8608..10836)
                     /gene="crfC"
                     /locus_tag="ACHKCL_003708"
     CDS             complement(8608..10836)
                     /gene="crfC"
                     /locus_tag="ACHKCL_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5764592.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2711 a         2848 c         2683 g         2604 t
ORIGIN      
        1 tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc
       61 aaccttgtat tgttttattg actgaaaaag atgtctggat aagggttaat gggaaagaac
      121 ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg
      181 tctcctctct taacaacact ttggttgctc atattagtca cgacatcatc aaagattacc
      241 tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca
      301 ttctcaccct gccatgcctg acaccagacg tctttcgcgt tgccgcgcaa cacagcatga
      361 tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat
      421 cccgttttct cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg
      481 taagtgacag cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta
      541 tggtagccag ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg aaaagcgaaa
      601 acaccagtta tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa
      661 tgatggacgg taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt
      721 actttatttc tgtctttaaa gacttctacg gtatgacacc gctgcattat gttagtcagc
      781 acagagaacg cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca
      841 tcagcgataa cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta
      901 atttattgtc acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct
      961 cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt
     1021 gtttttctgt tacctgcaaa cctggcctcc accggcggga ttgccattta tggatggttg
     1081 gtgacgatta tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgac
     1141 ccaagtcctg gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat
     1201 caaaccaacg tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt
     1261 ggcgtaggat atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc
     1321 acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc
     1381 acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg gattgccgta
     1441 tttggctggt tctggtttcg tggtgaaacc tatatggcgg catggaacgt cagcggcctg
     1501 ggcaccttcg gtgcaattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg
     1561 gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga aacgcaacgt ccctatagcc
     1621 accattggtg gggtattgat tgccgccgtt tgctatgtgc tctccaccac cgcgattatg
     1681 gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg
     1741 atggcgttgg gtgacaccgc cggggccatc gtttccttct gcgcagctgc gggttgctta
     1801 ggttcactgg gcggctggac attgctggcg ggtcaaacgg cgaaagcggc tgccgatgac
     1861 ggactgttcc caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg
     1921 attatcgtcg gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc
     1981 aaagaattcg gtctggtttc ttccgtgtcg gtcatcttta cactggtgcc atatctttac
     2041 acctgtgcgg cgttactgct gctcggacac ggtcactttg gtaaagcacg cccggcatat
     2101 ctggcggtta ctaccattgc cttcctctac tgcatctggg ccgtggtggg gtccggggcg
     2161 aaagaggtta tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat
     2221 tacaaccggc tgcataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc
     2281 cccttaatcc agcaaagata aaagccaacc ttaagaactt aaggttggct taattttgct
     2341 ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa
     2401 aagaccctct ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg
     2461 cctgaatatt gcctttttta aacaggtgtt gcaggcgctg ccgctggact cgctgcataa
     2521 cgtactggtt ttcttgtcga tgccggtcgt agccttcagc gtgattaata ttgtcctgac
     2581 actaagctct ttcttatggc ttaatcgacc gctggcctgc ctgtttattc tggttggcgc
     2641 ggctgcacaa tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa
     2701 tattattgat accactccgg cagaaagcta tgcgctgatg acaccgcaaa tgttattaac
     2761 gctgggattc agcggcgtgc ttgctgcgct gattgcctgc tggattaaaa tcaaacctgc
     2821 cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct
     2881 gattttgctg gtcgccgcac tattttataa agactacgcc tcgttgttcc gcaacaacaa
     2941 agagctggtg aagtccttaa gcccctctaa cagcattgtt gccagctggt catggtactc
     3001 ccatcagcga ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc
     3061 attaatgcag aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg
     3121 ggcggagaac ttctccctca acggctaccc gcgtgaaact aacccgcggt tggcgaaaga
     3181 taacgtggtc tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc
     3241 gtgcatgttc tcggatatgc cgcgagagca ctacaaagaa gagctggcac agcaccagga
     3301 aggcgtgctg gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg
     3361 cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc
     3421 tgatcagtgc atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta
     3481 catcaataac ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc
     3541 gacctattac aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa
     3601 tgagatccag acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta
     3661 cgtcgactat attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac
     3721 caccagcctg gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct
     3781 gcacggtctg ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg
     3841 gctgtcggag gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc
     3901 gcaaacgcaa cactattcac aagacaattt attctccacg ctattgggat taactggcgt
     3961 tgagacgaag tattaccagg ctgcggatga tattctgcaa acttgcagga gagtgagtga
     4021 atgaaaattc tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg
     4081 caaaccgaag gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt
     4141 gaggcaggtc attacagcct ggtggtactg gatttagggt tacccgacga agatggactg
     4201 cattttctcg cccgtatccg gcagaaaaaa tataccctgc cggtactgat cctcaccgct
     4261 cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg gtgccgacga ctatctggtg
     4321 aagccttttg cgctggaaga gttacatgcc cgtatccgcg ccctgctacg acgccataat
     4381 aatcagggcg aaagtgagct gattgttggc aatctgacgc tgaacatggg tcgccgtcag
     4441 gtatggatgg gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg
     4501 ttaatgctca aagcaggcag tccggtgcat cgggaaattc tctacaacga catctataac
     4561 tgggacaatg aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa
     4621 gtgggcaaag cccgtatccg caccgtgcgc ggctttggct atatgcttgt cgcgaatgag
     4681 gaaaactaat tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg
     4741 atattgacca tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg
     4801 catgaaagta ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac
     4861 gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc
     4921 tttatggtca gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg
     4981 ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc
     5041 attcacagcg ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt
     5101 cgcctgacca gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc gcacgaactg
     5161 cgaacgccac tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt
     5221 gatgtagcac cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg
     5281 caactggcgc gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg
     5341 gaagatgtca ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa
     5401 accctgctat tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg
     5461 cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac
     5521 attatgatta agctgcaaga agatggcgga gcggtcatgg ccgttgaaga tgaaggaccg
     5581 ggtattgatg agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt
     5641 tatggcggga ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg
     5701 cagtttttcc tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag
     5761 aaagatcaga acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac
     5821 acggtagtta aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag
     5881 gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag
     5941 cgttacatca ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg
     6001 cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat tttctgctcg
     6061 atattatcgt aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt
     6121 gttgcacctt tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca
     6181 accaccgcca ctaccatcag gtaataggct ggcatcatca gattctgcga gctttcgacc
     6241 agccaggccg ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc
     6301 gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg
     6361 cccgtaaagc agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg
     6421 acgttactgt taatcagaat aaacgccggg atcgccaaca caaacagtgc aacactacca
     6481 agtagcacaa acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca
     6541 aacagcatac cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc
     6601 aggttatgcg acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg
     6661 gcaattacca gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta
     6721 aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca
     6781 tgctgctgga acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct
     6841 aacggcagag caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg
     6901 acaatggtcg aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg
     6961 aagtccagcc agctgcccat aaagccacgt ttacggtcag gggagtattc cgcaacaaat
     7021 atcgacgccc cggtatattc accgccgacc gagaaacctt gtgccatctt acagatcaac
     7081 agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac
     7141 gtactgatcg acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg
     7201 cccaacatac caaagaagag tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg
     7261 gcaagtgcag caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat
     7321 gcgtaagcaa caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat
     7381 gctgcggtaa tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta
     7441 atcggtttta cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc
     7501 aaacgccaga aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat
     7561 ctctgtaaca agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac
     7621 cagttgacgc ctgaattggg cagttaatga cctttttacc ctaccagcgt ttaaatttgt
     7681 gatcaacttc acacatggat ttcgtttagg actcattgat gtaactgatt atttacacca
     7741 aatttacaga gaagatttcc tcgagcattg cccccgccag acgctgcgct gaacagagtc
     7801 gtggcatccc cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa acgggttgtt
     7861 tatctgccgt ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg
     7921 gaacggtcga agaggcaaac cactgatggt aatagtgacg cgtggtcggg gccgcattga
     7981 tactctctgc cagatgttgt gtcgctttca cgcgcaaatc acccagcaac aacgccaccg
     8041 cttgcgggct tcccagacga caggcatcgc caaatgcccc ccaacgcaac tcttcgagaa
     8101 cgataaaaca gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa
     8161 aaatttgctc gctctgttca cccgcctgca ccagtaggcc tcgttgcgtt aacgcctttt
     8221 ccttccagcg acaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct
     8281 ccgggaaagc agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga
     8341 tctgcgtcag tgccagatga ccgggatcca gcatcgacgc cagtttttcc accaatgtca
     8401 gacgcagact aagcggcgca tttaacatcc cgccgttcgt ccatgtctga agctgcacgc
     8461 gcgcaagaag ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgtggaaggc
     8521 gggcatcatc gccctgcgcc agatccacta aaaagcgggg ataaaccgac tccagcacgc
     8581 gaccggggcc gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat
     8641 atcatcgcgt aacagctgag cgtcggtgta aatccagccg tgagtcatca cagtctgctg
     8701 caattgctgt tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc
     8761 gcgtaaacgc gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat
     8821 gcctgccgta acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata
     8881 aagatcgact tctgcgctta atgctcgttg caacacactg taatcaaccg tttctgtcgg
     8941 ggatttctca tttccccgtc cccagtcagg ctgattcaac cagcgcgaaa aagtctcacg
     9001 cacaacgcct aatcgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact
     9061 gaacagctgg cgagtgttga agtggggaac tacgccgtga aaaacaggaa aatgaaaccc
     9121 aggacgaaac cctgactcgc tcaattccat tttgacttgt tgctcaatgg ggcgaataac
     9181 atcggttaac acccggcaaa gggtggattc aagctcggca aaacgcagcg taaagtcgcg
     9241 actgatggtg ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa
     9301 cggctccgaa tcatcctgaa acaaggcggc taactgcacc ttcagcgcat cttgctgttg
     9361 acgcagaaag tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct
     9421 cacctgcgcc tgattgagtt gcaatagctg caaactttct tcgatctggt ggatattttg
     9481 ccgcaattgt tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc
     9541 ctgctgcgcg taattcaaca gtttatgcgc ggcagatcgc agagcataca acgaggcgtt
     9601 agcgtaagcg gcatgaagca acatctgaat tggctgggcg aacagcgaat cttcccacaa
     9661 ctgatcggca gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct
     9721 gccgagcgcg gcatgggcaa aatcttccac ccagcgttgt tgctctggcg gtggtaactt
     9781 accgttgttg gctaactcat ggcgcgcccg atttgccagg tagccccaca tcgacgacac
     9841 cggaaatatc tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg
     9901 cacctggtcg gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata
     9961 cagcggcacc gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga
    10021 tttcagttgc gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt
    10081 aagcattttt tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt
    10141 caattgaccg ggataacttt ccagccccgc cagatggaca aactccactt caatcacggg
    10201 aatatgctca atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag
    10261 tcgcaccaaa tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata
    10321 tttttcgaaa gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat
    10381 ttccagcaca tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaatttttg
    10441 aattaaacaa tcgattggcg cgacatgtga aaaatgcagt accggttcct tttggccggg
    10501 cgtatggcga ataagcgtcg gcagcgcagt cattgggcga ttgcgattag gcagtacctc
    10561 cgtaccaaca atggcattaa tggtggttga tttccctgct ttcatggtac cgacaatcgc
    10621 cagcaccatt tccagtcggg aaattttacg caactcatta ttcagcatcg cgtgacgctc
    10681 ggcgatatta ggctgactcc agggtaaagc cagttgtggc gcatcgtctc cgggtacaga
    10741 gagaggcatt ttttccagta actgcaactg ttggcgagaa agctgtaaca gccgttcagc
    10801 ctcctgactt aactcataca gggtctgtgt gtacatagaa aattct
//
LOCUS       DAZFKY010000160        10765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFKY010000160 DAZFKY010000000
VERSION     DAZFKY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..104
                     /locus_tag="ACHKCL_003709"
     CDS             <1..104
                     /locus_tag="ACHKCL_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764593.1"
                     /translation="AADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            315..638
                     /gene="ybjQ"
                     /locus_tag="ACHKCL_003710"
     CDS             315..638
                     /gene="ybjQ"
                     /locus_tag="ACHKCL_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5764594.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            635..1465
                     /gene="amiD"
                     /locus_tag="ACHKCL_003711"
     CDS             635..1465
                     /gene="amiD"
                     /locus_tag="ACHKCL_003711"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5764595.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1462..2475)
                     /gene="ybjS"
                     /locus_tag="ACHKCL_003712"
     CDS             complement(1462..2475)
                     /gene="ybjS"
                     /locus_tag="ACHKCL_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5764596.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2574..4004)
                     /gene="ybjT"
                     /locus_tag="ACHKCL_003713"
     CDS             complement(2574..4004)
                     /gene="ybjT"
                     /locus_tag="ACHKCL_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5764597.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(4015..5016)
                     /gene="ltaE"
                     /locus_tag="ACHKCL_003714"
     CDS             complement(4015..5016)
                     /gene="ltaE"
                     /locus_tag="ACHKCL_003714"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5764598.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5053..6771)
                     /gene="poxB"
                     /locus_tag="ACHKCL_003715"
     CDS             complement(5053..6771)
                     /gene="poxB"
                     /locus_tag="ACHKCL_003715"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5764599.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6904..7872)
                     /gene="hcr"
                     /locus_tag="ACHKCL_003716"
     CDS             complement(6904..7872)
                     /gene="hcr"
                     /locus_tag="ACHKCL_003716"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5764600.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7884..9536)
                     /gene="hcp"
                     /locus_tag="ACHKCL_003717"
                     /gene_synonym="priS"
     CDS             complement(7884..9536)
                     /gene="hcp"
                     /locus_tag="ACHKCL_003717"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5764601.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9680..10579)
                     /gene="lysO"
                     /locus_tag="ACHKCL_003718"
     CDS             complement(9680..10579)
                     /gene="lysO"
                     /locus_tag="ACHKCL_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5764602.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2587 a         2868 c         2883 g         2427 t
ORIGIN      
        1 cggcggcgga ccaaaaagca atccagcggt atatacctca ggcgcaagat ggggaagact
       61 ttaggaatca tttacaatat aagttgactc ttcccactca ttaatgttgc cggcacaaca
      121 taatagggca ggtaagcggt gctccataat gaaaccaata tccggttacg aaacagataa
      181 ttgtacaggc ttatgtagct gtttttgaca caatgagatg atttcctaaa ctgatcaaaa
      241 ccttcttccc aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa
      301 caaggagatc gatcatgcaa ttttccacaa ctccaactct ggaaggccag accatcgttg
      361 aatattgcgg tgtggtgact ggcgaagcga ttttaggcgc gaatattttc cgtgatttct
      421 ttgctggtat tcgcgatatc gttggcggac gctccggtgc ctacgaaaaa gagctacgta
      481 aagcgcggga gatcgccttt gaggagcttg gctcccaggc gcgggcgctg ggggccgatg
      541 ccgtagtcgg tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg
      601 ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctgatcgc
      661 tgtcgctctg ttactggcag ggtgcgcagg cgaaaaaggc attgtcgaga aagagggata
      721 tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat
      781 ccactacacc gcagatgatt ttgatagctc gctggcgaca ctgaccgata agcaggtcag
      841 ctcgcattat ctggtccctg cggtaccacc gcgatacaac ggtaaaccgc gcatctggca
      901 attggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac
      961 gcgccttaac gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcggc
     1021 gggggtgaaa tattttgccc cgtttgaacc ggcacagatt caggcgctta ttccgctggc
     1081 gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat
     1141 cgccccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca
     1201 ggggattggt gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc
     1261 gcacacaccg gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt
     1321 taaacctgat atgacaccgc gcgagcagcg gcgcgtgatt atggcgttcc agatgcattt
     1381 ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcgtt
     1441 gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgca gccaggcggc
     1501 agttttctcg ataccttcat ccagggtgat gaccggctga taacctaact cttcctgtgc
     1561 ccgcgtaata tccagcgtaa agtcaaaatt aagcttcgag acgccgtagt gggtcagcgg
     1621 cggctctttt gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat
     1681 cgggtaaggg acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac
     1741 gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag
     1801 cttatcgcag gcttcctggc ttgccagcca catggcgtgc acggcatttt catagtaggt
     1861 catatccacc agcgcactgc cgccatgcgg taacagaatg ctgccgtagt ggtgcatcat
     1921 atgcgcaaga cgggggataa agactttatc gtgcggtccg aacagacttt gtgggcgcag
     1981 aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc
     2041 cgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatc
     2101 gcgatggtgg tgataatcga agtacaggga gggagaagag atatgaataa agttacgtac
     2161 accccaggcg acagcccatt cacctaggcg gcgagtggcg cgaacgttag ccagatcgaa
     2221 agcctgttgt gtcccccagg gagaggtaaa gctggagcag tgccacagcg tatcaatgcc
     2281 cgcgagcatc actttagctt gtgatgagac cagctcggtc agatccgccg gaacaaactc
     2341 tgcgcccatt ttttccagca atttacccat tgcctcgttg cgaccggtcg ctcgcacgct
     2401 gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt
     2461 aaccagtacc ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg
     2521 atttccccct tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct
     2581 gtgctttgtt ctgccagtct ggcgattcgt tttgccattc cgcgaaaaat aaacagatgc
     2641 gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg
     2701 cggacatcga tagtacgata gtcgccttta tcttccaggg taaaacacag tcgtcccagc
     2761 cccggtgctt tcatgccaaa taacaacgta agttgttttt ccggttcaac gacaatcact
     2821 ttccagctat ccaccgcatc gccagtttgc aaatattcgc gctccggacg gcctttcgct
     2881 aatttatgac cgatcgcacg gtccatcaac gcccgtgtct gccacaaaat attgccaaag
     2941 aaataacgct ctttaccgcc gatttggttc accacctgcc ataaagccgc aaggctggcg
     3001 gacgttttaa cggtaaatcc cgcctgtttg gcaaaataac catactctgg tcgccagcgg
     3061 gcaaaggcct gagtgtcgta tccccagtcg ctggagttga ccagcttttc ttcttctttc
     3121 agggtgcgac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag cgcacgtagc
     3181 gcggtgtcat ccgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg
     3241 ggcggtacgg aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg
     3301 atggggatca accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga
     3361 taactgagca cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct
     3421 aacagcgcca ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt
     3481 acccagcgtg gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac
     3541 gctgctgaac ctgcaccaac gattattccg gcacgcaatt cggtcactgg tacattcgct
     3601 tcacgaagaa tgtccgccgt agcctgacgc gcgcgcagat ggtccgactg ctcatgtggc
     3661 ggggcctgca acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg
     3721 cggaagttga gagccacctg gcgctcctga gcgataaaat cgccgccttc gcccatgcta
     3781 tgcaccagaa aatagaccgt atcgatatcc tgtaacaggg ccggaaggtt atccggccag
     3841 ctgagatcga ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca
     3901 tgacgtgccg ccgccaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga
     3961 ccaatgtagc cactggcacc aagaactaaa atgcgttgcg gcacgtctcc ctccttaacg
     4021 cgccaggaat gcgcgccagt gggcggcgac ttctgccagt tgttcgcgcg agacgtcaag
     4081 atgggtcacc aggcggacaa tcggcgaggc gttaatcagt acgtttctcg ctttcatgta
     4141 ttcgcctaac gcggcagcat tttcttcgcc gacgcgaaca aacagcatat tagtgtcctg
     4201 acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg
     4261 atcttcctgc aaccgtgcga cgttattttt cagcgcatac atcccggcgg cagccagaat
     4321 gccggactgg cgcatcccgc cacctgtcat tttccgccag cgaatggcac gtttaatgta
     4381 atcacgatta ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat
     4441 ggtgaacgaa tcacaatatt gcgtgagctc tttcagttcg ctgccgtaag ccaccacggc
     4501 attaaagatg cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca
     4561 tgcatcttta aggtattcac gcggcaacac tttgccgttg tgggtgtttt ccagactgag
     4621 taatttggtg cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag
     4681 cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggt tgaatactgc ccagcaccgc
     4741 cgcgccaccg gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc
     4801 gcgttcgcag tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat
     4861 ggcggcttct ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc
     4921 gtctccgtaa acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct
     4981 cggtcgggtt acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa
     5041 atgccaccct ttttacctta gccagttcgt tttcgccagt tcgatcactt catcaccgcg
     5101 tccgctgatg attgcgcgca gcatatacag gctgaaacct ttggcctgtt cgagtttgat
     5161 ctgcggtgga atggctaact cttctttggc gaccaccaca tccaccaata ccggaccgtc
     5221 gatggagaag gcgcgttgca gggcttcatc gatttcagag gctttttcta cacgaatacc
     5281 cgtaattccg cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc
     5341 tgtcaggtag cctccggctt tcatctccat cgccacaaag cccagcacgc tgttattaaa
     5401 gacgataatt ttcaccggca gtttcatctg cactaccgag aggaaatcgc ccatcaacat
     5461 gctaaaaccg ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc
     5521 gcccagcgcc tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg
     5581 acgcttgccg ttcattttca gataacgcgc cgcccacacc gttggcgtgc cgacgtcaca
     5641 ggtaaagatg gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg
     5701 aatggctttc tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc
     5761 ttccagcgct ttatccagaa acttgcgatc ggctttttct tccaccaatg gcagcaatgc
     5821 acgcagggtc gacttgatat cgccgaccag cgccatatcc accttgctgt gtgcgccgat
     5881 gctggctggg ttgatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta
     5941 ggggaactga gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga
     6001 cgagaatccg attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc
     6061 tttaccgcgt agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc
     6121 tttatgcgcc cccgcgcagc cgctgccaca catcagagcg atattgctgg aataacgcag
     6181 cagttgcgcc agtttgcgta actcttcttc ttccggcgtc acgactggct gtggcgcatg
     6241 ataccagtgg gtagttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaacac
     6301 gacaacggaa acgccacggt taagcaccgc tttgcgcatg gcaatcgcca gcacttgtgg
     6361 gatctgctcc gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg
     6421 tgggtgggtt tcctggaaat agccgctgcc aatttcgctg gaaggaatat gagcggcaat
     6481 cgccagtacc ggaacgtgat tgcggtggca atcgaacagg ccgttgatta agtgcaggtt
     6541 gccggggccg cacgatccag cacagaccgc cagttctcct gaaagttgtg cttcagcgcc
     6601 agcggcaaac gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg
     6661 attaagactg tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac
     6721 gcctgccgat tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca
     6781 tctcctgaat gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg
     6841 agagaaagga ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg
     6901 cgattatgcg agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc
     6961 agcgatttcg gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac
     7021 tttcgtctta cagcagccgc aaacacccgc gcggcaggcg gcgacaaccg gaacgttatt
     7081 gctttccagc gcctccagca gcgtggtgcc aacaggagcg taaaattctt gcgccggttg
     7141 cagtttggtg aatttcagac cgctggtcgc agcttccgct actggggtga agaatttctc
     7201 tttaaagaaa cgcgtcacgc cgagtgcttt cacttcctgc tctacccaat ccatatacgg
     7261 agccgggccg caggtcatca cggtacgtga agctaagtca ggaacgcgag tcaggagttc
     7321 gcgagtgaga cgaccagcga taaagccttc ggtaacgtta ttttccgcca ccagcgttac
     7381 cggatagtta cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat
     7441 cacccgcaca tcggcctgtg gacggttctt cgccagccag cgacgcatcg acataatcgg
     7501 cgtgacgccg cagcctgcag ccagcaacag gaatttatct tctgctttat cgtcgcaggt
     7561 aaattccccc atcgcgtctg aaagccagag ataatcaccg cgttttacat cgcgggtcag
     7621 ccactgggag ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac
     7681 gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc gctgagttac gcacgctgac
     7741 cagtgcatat tgcccggcgc ggtatgggta gtaatcgtgg caaatcaggg aaatcgtcca
     7801 cacatccggc gtttcttgct taatgtgatg aacctgcatc cgccacgggc attgattcgt
     7861 tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca
     7921 gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg ccagcagatc tggtgtcagg
     7981 aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc
     8041 agaatgacaa tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttg
     8101 acaccgcagc ccagtttttc tgccagcgta accgccagaa taattgctga gtaagcatcg
     8161 ttacactgac cggcatccac cagacgaggc agaccttcga tatcgccaaa ctcaagtttg
     8221 ttaaagcgat atttaccaca ggcgagagtc aggatcaggc agtcatctgg cacgctggtg
     8281 gcgaaatcgg tgaagtagtg acgttcgccg cgtgcgccgt cacagccacc aagcaggaag
     8341 atatgacgca gtttttcacg gctcaccaga tcaattagcg tatccgctgc gccaagcagc
     8401 gtctggcggc caaagcccac ggtgatcagg tgcggaattt cgctgtatgg gaagcctgcc
     8461 atttgttgcg cctgggcaat gaccgcagag aaatcttcgc catccagatg acgcacgcca
     8521 ggccagccaa cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg
     8581 atgatgcagt tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga
     8641 ttctgccagc cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag
     8701 ccatgcgcag gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt
     8761 tccagcaggt tgtagagatc tttgagatcg tgaccggaaa tcaggatgca tttacccgcc
     8821 gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca
     8881 tccagaatgc tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc
     8941 gcgttcatat cggcaggcca ggtccccagc cacgccatga ttttatgata ctgggcataa
     9001 atatcgttgt cgtattgacc gagaacgtgt gcgtgttcca tataggccgc cgcacctttc
     9061 aggccataca ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct
     9121 ttgttagggg taaattctgc tgcctgacgt tgcagttcgc cgagatcgtc gctcaccagt
     9181 tgcaggtcag ccatcgggtt atcgacgcgg gcgttggcat ctacagccag gcattgtgct
     9241 ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg
     9301 aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc gacatcgtgg
     9361 ttgatgatgc cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg
     9421 atgagtaaat cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat
     9481 gagcagccgt ttcctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc
     9541 acacctttta aagttatatt taatatacat gtttaaggtt aagacgctta acgcggggat
     9601 aaaagggatt tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg
     9661 atctaccgcc agagaggtat tacgcggaga aaaaggcgat gaggatcggc actaacaggc
     9721 taagaataaa accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa
     9781 gaacgggcag ggtgaaatcc atcgacgtcg caccgcataa gcccagtgcg gtagagcggc
     9841 tgcggcgaat cagcccaggg atcaacataa ttgcaatcag ctcacgggcc agatcattaa
     9901 aaaacgccgc gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag
     9961 aataccagcc gaaaccagag gccattgcca gcgcggtatt gatgggtaga tcaagaataa
    10021 aggcgttaat taaaccacca attaatgaac tggcaaccac caccacggcg acaatcattc
    10081 cccggcgatt aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccga
    10141 cgaggaaaag tagcagaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag
    10201 ccagtccact caggccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact
    10261 caagcgccat cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca
    10321 gaccgcgctc cagccacata agggcggcaa tattacacag taaaataacg gtaatactga
    10381 cggcagaata atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac
    10441 tgatacccat aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaataactt
    10501 ttaacgcagc ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga
    10561 ttaacaaccc agaaaacatg aaaaaccagc ccttgcaaag aaagaagtcg aaatgcgcga
    10621 tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca
    10681 tcagaggggg atttttaatt ctatatgagt gtttagcatt gttgcgctcc tttcatgtgc
    10741 aatgccatat aaagccactg gcgca
//
LOCUS       DAZFKY010000161        10727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFKY010000161 DAZFKY010000000
VERSION     DAZFKY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..840
                     /locus_tag="ACHKCL_003719"
     CDS             <1..840
                     /locus_tag="ACHKCL_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5764603.1"
                     /translation="HEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTI
                     KAFSFGGDLQTLASKPSSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLA
                     MAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHA
                     LYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGAWPNKL
                     QPGKYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPLNY
                     LYL"
     gene            1177..2154
                     /gene="glaH"
                     /locus_tag="ACHKCL_003720"
     CDS             1177..2154
                     /gene="glaH"
                     /locus_tag="ACHKCL_003720"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5764604.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2174..3442
                     /gene="lhgO"
                     /locus_tag="ACHKCL_003721"
     CDS             2174..3442
                     /gene="lhgO"
                     /locus_tag="ACHKCL_003721"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5764605.1"
                     /translation="MYDFVIIGGGIIGMSTTMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3465..4913
                     /gene="gabD"
                     /locus_tag="ACHKCL_003722"
     CDS             3465..4913
                     /gene="gabD"
                     /locus_tag="ACHKCL_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5764606.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4927..6207
                     /gene="gabT"
                     /locus_tag="ACHKCL_003723"
     CDS             4927..6207
                     /gene="gabT"
                     /locus_tag="ACHKCL_003723"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5764607.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6445..7845
                     /gene="gabP"
                     /locus_tag="ACHKCL_003724"
     CDS             6445..7845
                     /gene="gabP"
                     /locus_tag="ACHKCL_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5764608.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7866..8528
                     /gene="csiR"
                     /locus_tag="ACHKCL_003725"
     CDS             7866..8528
                     /gene="csiR"
                     /locus_tag="ACHKCL_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5764609.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8529..8978)
                     /gene="kbp"
                     /locus_tag="ACHKCL_003726"
     CDS             complement(8529..8978)
                     /gene="kbp"
                     /locus_tag="ACHKCL_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5764610.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9062..9220)
                     /gene="yqaE"
                     /locus_tag="ACHKCL_003727"
     CDS             complement(9062..9220)
                     /gene="yqaE"
                     /locus_tag="ACHKCL_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5764611.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9403..9702
                     /gene="ygaV"
                     /locus_tag="ACHKCL_003728"
     CDS             9403..9702
                     /gene="ygaV"
                     /locus_tag="ACHKCL_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5764612.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9712..10230
                     /gene="ygaP"
                     /locus_tag="ACHKCL_003729"
     CDS             9712..10230
                     /gene="ygaP"
                     /locus_tag="ACHKCL_003729"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5764613.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(10283..10687)
                     /gene="stpA"
                     /locus_tag="ACHKCL_003730"
     CDS             complement(10283..10687)
                     /gene="stpA"
                     /locus_tag="ACHKCL_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5764614.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2534 a         2696 c         2875 g         2622 t
ORIGIN      
        1 catgaaaaag ccgttatgaa ttacatggaa tatctggtaa cttgccagtt ggatgaagaa
       61 caaatgtcat cgctgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc
      121 atgaagacga taaaagcttt ttcatttggt ggcgatctcc aaacccttgc atcaaaaccg
      181 agttccacaa tctcaagcat cccatcaaaa agacggatat tgattaacat taaccacgat
      241 tttcctaaca atggcaatct tttcaatgac tttctattta accatcaaca agatgaacaa
      301 ttagcaatgg catatatggc cgctctcccg ttcagcaggc ctttagttta ctgggatggc
      361 caagtattaa aatcaacgac tgaaattaaa aattatgatg ggtcgacgcg tgtcggcggt
      421 gaggcgtggc ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca
      481 ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac
      541 gttctggcct ttagtcgtgg ggattcggtg aacattaatc actctcctca tgatggacta
      601 gttataataa ataaaggaaa cgaagaagtt gaaggtgcct ggcctaacaa attgcaacct
      661 ggaaaatata aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa
      721 attatccccc ctggtaaagc atttatgctt agaggcggaa ctctaaatat caatattcct
      781 gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc tgaactatct ctatttgtaa
      841 tttaatgtta tatctgcccc gctaaaacgg ggcagataat atatttagtt tactagcggt
      901 cattttgcag tgaagccatt taccgttttt tatcgaccag ataatctgtt ctctgatggt
      961 aatccccccc taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc
     1021 ctacgttaaa tgttaccaat ttgttgcttt ttatcacaat aagaaaacaa tatggcgcat
     1081 ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc
     1141 catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa
     1201 aataacgctg tcgattcaga ccaggactat agcggattca ctctcatccc gtcggcgcaa
     1261 tccccgcgtc tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag
     1321 gttgccgaat ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggt
     1381 aaaattcttg acgatctgtg tgcgaaccag ctgcaaccat tgctgttgaa gaccctgcta
     1441 aaccgtgctg aaggtgcgct gttgatcaat gcggtgggtg tcgatgatgt gaagcaagcg
     1501 gatgagatgg tgaagctggc gacggcggtg gcgcatctga ttggccgttc caatttcgat
     1561 gcgatgagcg gtcagtatta cgcgcgtttt gtagtgaaaa atgtcgataa ctcagacagc
     1621 tatctgcgtc agccgcaccg cgtaatggag ctgcacaacg acggcaccta cgtcgaagag
     1681 atcactgact atgtgctgat gatgaaaatc gacgagcaga acatgcaggg tggaaattcg
     1741 ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca tccaatggcg
     1801 cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat
     1861 ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga ccagttcgtc
     1921 cagccaaaag acttcgaaga aggcgtgtgg ttgagcgagc tttcggacgc cattgaaatc
     1981 agtaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat taacaacctg
     2041 ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg
     2101 cgtcagcgtg gctatttcgc ttacgccact caccactatc agacgcatca gtaagcgcaa
     2161 aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac
     2221 caccatgcaa ctgattgatg tctatccgga tgcccgcatt gcgttgctgg aaaaagagtc
     2281 cggcccggcc tgtcatcaga cgggccacaa cagcggcgtg atccatgccg gggtttatta
     2341 cacgcccggt agcctgaagg cgcagttttg cctggcggga aaccgcgcca ctaaagcctt
     2401 ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacctctga
     2461 actcgaaatg gaacggatgc gcgcgttgtg ggaacgcacg gcggcaaacg gtatcgagcg
     2521 cgagtggtta aacgccgatg aactgcgcga gcgcgaaccg aatatcaccg ggcttggcgg
     2581 tatttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtcacggcgg cgatggcaaa
     2641 aattttccag gccagaggcg gcgagattat ctataacgcc gaagtcagcg gccttagtga
     2701 gcataaaagc ggcgtggtga tacgtacccg tcagggcagc gactatgaag catcgacgct
     2761 gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg
     2821 ctttattatc tgcccgttcc gcggcgagta tttccgcctg gcgccggagc ataaccagat
     2881 tgttaaccat ctgatttacc ccattcccga cccggcaatg ccgtttttgg gcgtgcatct
     2941 cacccgcatg atcgacggca gcgtcaccgt cgggccaaac gcggtgctgg ctttcaaacg
     3001 cgaaggctat cgcaagcgcg acttctcatt tagcgacacg ctggagattt tgggctcgtc
     3061 ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga tgaaaaactc
     3121 gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag
     3181 cgatctccag ccctggcccg ccggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct
     3241 gattgacgat tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc
     3301 cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct
     3361 gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt
     3421 acacgccgcg tttaatcaat aacctttgaa aacaggatgt agcgatgaaa ctgaacgaca
     3481 gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggtg
     3541 aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg
     3601 gcgctgatga aactcgcgcc gctatcgacg ccgccaaccg cgccctgccc gtctggcgtg
     3661 cgctcaccgc caaagaacgc gccaacattc tgcgcaactg gttcaatttg atgatggagc
     3721 atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg gctgaagcga
     3781 aaggcgaaat cagctacgcc gcctctttta ttgagtggtt tgccgaagaa ggcaaacgca
     3841 tttatggcga caccattccc ggtcatcagg ccgataagcg cctgattgtg atcaagcagc
     3901 cgattggcgt tacagcggcc atcacgccgt ggaactttcc ggcggcaatg attacccgca
     3961 aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc agtcagacgc
     4021 cgttctctgc gctggcgctg gcggagctgg cgatccgcgc gggcattccg gctggcgtgt
     4081 ttaacgtggt caccggttcg gcgggcgcgg tgggtaatga actgaccagc aacccgctgg
     4141 tacgcaaact gtcgtttacc ggctcgaccg aaattggccg ccagttaatg gaacagtgcg
     4201 cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg
     4261 acgatgccga tctcgataaa gccgtggaag gcgcactggc ctcgaaattc cgcaacgccg
     4321 ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg
     4381 ccgaaaaatt gcagcaggcg gtgagcaaac tgcatatcgg cgacgggctg gataaaggcg
     4441 tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag catattgccg
     4501 atgcgctgga aaaaggcgcg cgcgtagttt gcggcggtaa agcacatgaa cgcggcggca
     4561 acttcttcca gcccaccatt ctggtggacg tgccggccaa cgccaaagtg tcgaaagaag
     4621 agaccttcgg ccccctcgcc ccgctgttcc gttttaaaga tgaagccgat gtgatcgcgc
     4681 aagccaatga caccgagttt ggccttgccg cctatttcta tgcccgtgat ttaagtcgcg
     4741 tcttccgcgt gggcgaggcg ctggagtacg gcatcgtcgg catcaatacc gggattattt
     4801 ccaatgaagt ggccccgttc ggcggcatta aagcctcggg tctgggtcgt gaaggttcga
     4861 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
     4921 acgcgaatga gcagcaataa agagttaatg cagcgccgca gtcaggcaat tcctcgtggc
     4981 gttgggcaaa ttcaccccat tttcgctgat cgcgcggaaa actgccgggt gtgggacgtt
     5041 gaaggccgtg agtatcttga tttcgcgggc ggcattgcgg tgctcaatac cgggcacctg
     5101 catccgaaag tggttgccgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc
     5161 caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg
     5221 ggcgattttg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg
     5281 gtgaaaatcg cccgcgctgc caccaaacgt agcggcacca tcgcttttag cggcgcgtat
     5341 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     5401 atgggcctga tgccagggca cgtttatcgc gcgctttatc cttgcccact gcacggcatc
     5461 agtgaagatg atgctatcgc cagtatccac cgaattttta aaaatgatgc tgcgccggaa
     5521 gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta cgccgcgacg
     5581 cctgcgttta tgcagcgttt acgcgcgctg tgtgacgagc acgggatcat gctgattgcc
     5641 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcaaatgggc
     5701 gtggcaccag atctcaccac ctttgcgaaa tcgatcgcag gcggcttccc actggcgggc
     5761 gtcaccgggc gcgccgaagt gatggatgcc gtcgctccag gcgggctggg tggcacctat
     5821 gccggtaatc cgattgcctg cgtggcggcg ctggaagtgt tgaaggtgtt cgagcaggaa
     5881 aatctgctgc agaaagccaa cgatctgggg cagaagttga aagatggatt gttggcgatc
     5941 gccgaaaaac accctgagat cggcgacgta cgcgggctgg gggcgatgat cgccatcgag
     6001 ctgtttgaag acggcgatca caacaagccg gacgccaaac tcaccgccga gatcgtggct
     6061 cgcgcccgcg ataaaggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     6121 atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga gatcatcagc
     6181 cagtgttttg ctgaggcaaa gcagtagcgc cgctcctatg ccggaggcga cgctgcgcgt
     6241 cttgtccggc ctacggggat ccaggtcgga taaggcgttt acgccgcatc cgacaatctg
     6301 tacgtgaaca ggaataaatc tatgttggcc gggtaaggcg gagccgctct ccggcaaaaa
     6361 gaatcaataa caattatacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt
     6421 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc
     6481 gggctgaagt cacgccatgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     6541 tttgtcggtt ccagcgtcgc catcgccgaa gcgggcccgg cggtattact ggcctatctg
     6601 ttcgccgggc tactggtggt tatgattatg cggatgttgg cggaaatggc ggttgccacg
     6661 cccgataccg gttcgttttc cacctatgcc gataaagcta ttggtcgttg ggcgggttat
     6721 accatcggct ggttgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc
     6781 gccgccatga tcctgcactc atgggttcca ggcattccca tctggttatt ttccctcgtc
     6841 attacccttg ccttaactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag
     6901 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     6961 gcaattagcg gtttttaccc gtatgccgac gtgagcggga tctcaagatt gtgggatagc
     7021 ggcggcttta tgcccaacgg cttcggtgcg gtattaagcg cgatgttgat caccatgttc
     7081 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacac gccggaaaaa
     7141 catattgtcc gcgctaccaa ctcggttatc tggcgtattt ctatcttcta tttgtgctct
     7201 atttttgtcg tagtggcgtt aataccgtgg aatatgccgg ggctgaaagc cgttggatct
     7261 tatcgctcgg ttctggaatt gctcaatata ccccatgcga aattaatcat ggactgcgtg
     7321 atattacttt ccgtaaccag ttgcctgaac tcggcgctgt ataccgcgtc aaggatgctc
     7381 tactccttaa gccgtcgcgg tgatgcgccc gcggtaatgg gcaaaatcaa ccgcagtaaa
     7441 accccgtatg tggcggtgtt actctccacc ggagcggcat ttttaacggt cgtggtgaac
     7501 tattacgcac ctgcgaaagt gtttaaattc ctgatcgaca gctccggtgc tatcgccctg
     7561 ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gtaaaattct gcgagcagaa
     7621 ggaagcgaaa ttcgcttgcg catgtggctt tacccgtggc tcacctggct ggtaatcggc
     7681 tttattacct ttgtgttggt ggtgatgtta ttccgcccgg cgcaacagtt agaagtgatc
     7741 tctaccggct tattagcgat agggattatc tgtaccgtgc cgattatggc gcgctggaaa
     7801 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg atcaggagtc
     7861 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     7921 gtaattttca accggatgaa aaattacgaa tgagtttgct gacatcgcgt tatgcacttg
     7981 gcgttgggcc gttacgggaa gctctttcgc aactggtggc ggaacggctg gtcacggtgg
     8041 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
     8101 acgcccgcgc caatatggaa gcgatgttag tgagtctggc gattgcccgc ggtggcgatg
     8161 agtgggaggc agacgttctc gcaaaagcgc atctgctgag taagcttgag gcctgtgacg
     8221 ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat actgcaattg
     8281 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
     8341 cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg ctggaggata
     8401 aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc gcgcgcgcca
     8461 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
     8521 gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt atccgggctt
     8581 tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc
     8641 tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagtg
     8701 gctggtgtcg ccgttttcac ctgatcatca acactggcaa taccggaaat attccccacc
     8761 gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac cgtcgctttg
     8821 ccatcggcaa tttgaatgtt cactttatcg gcatcaggta taccggtttt gctcagatgc
     8881 tcctgcacct tcttcgcctg atcatcttta tcgtgctgac ctgtaaccgc gtcccagagt
     8941 ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga
     9001 aacactcaga aagtgtagta gaggaacgcg gcgcgttaac gccgcgggga tgagcacagg
     9061 tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg
     9121 tcaacagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg
     9181 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
     9241 ttacttattg tttaaaaaga gattttatct cttaagcgta gtaattttag cggaggctgc
     9301 cagtttcagc ttacgctttt actctcattt acgctgctga ggctggtata tgttgcattt
     9361 ttagtttaga gtttacttaa tttagaaaat acttaaataa ctatgaccga actcacgcaa
     9421 ttacaggcca gtgctgaaca ggcagcggcc ttattgaaag caatgagcca ccctaaacgg
     9481 ttgctgattc tgtgcatgct tagcggttcc cccggcacca gcgcgggaga gctgacgcgc
     9541 attaccggac tgagcgcctc tgcgacatca cagcatctcg ctcgtatgcg ggacgaaggg
     9601 cttatcaaca gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta
     9661 aatgccatta tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg
     9721 acaaccattt cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat
     9781 attcgtgacg ctgatgaata tcttcgtgaa catattcctg aagcagatct ggctccatta
     9841 tccgtgctgg aacagtcagg tctcccggct aaattacgtc gcgatcaaat tatattccac
     9901 tgccaggcag gtaaacgcac cagtaataac gccgataaat tagcagcgat tgccgccccc
     9961 gcagaaatct ttttactcga agatgggatt gatggctgga aaaaagcagg attaccagta
    10021 gcggtaaata aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt
    10081 ttaatattaa tcggcgttat actgggttat accgtaaata gcggtttctt cttattaagc
    10141 ggctttgttg gtgccgggtt actgtttgca ggaattagcg gtttttgtgg gatggcaagg
    10201 ttgttagata agatgccgtg gaaccaatga gcttgagaag cgacgccgga cgcgccctag
    10261 cagcgacatc cggcctcagt aattagatca ggaaatcgtc gagagattta ccttctgcca
    10321 gcgcctgagc gattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt
    10381 taacatcggt gaatttatat ttcgccggac gcggctggcg ttttttacca gcacgtggtg
    10441 cagcagcaga gctattaccc aataactctt ccgggttaat tccgtcagct ttcatcagct
    10501 ccagccaggt gctaattttt tcctggcgct ctgccagttc acgctgctgc tgttcttctt
    10561 cttcacgtct ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa
    10621 tggagaattc gcgagccatc gcacggaggg tgcgaatgtt atttaaactt tgtaacatta
    10681 cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa agtattt
//
LOCUS       DAZFKY010000162        10581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFKY010000162 DAZFKY010000000
VERSION     DAZFKY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..208
                     /locus_tag="ACHKCL_003731"
     CDS             <1..208
                     /locus_tag="ACHKCL_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5764615.1"
                     /translation="VDPFNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDR
                     ESVFWLNVKNIPPSIDKKIPMFLR"
     gene            361..510
                     /locus_tag="ACHKCL_003732"
     CDS             361..510
                     /locus_tag="ACHKCL_003732"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764616.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            532..3066
                     /locus_tag="ACHKCL_003733"
     CDS             532..3066
                     /locus_tag="ACHKCL_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5764617.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3078..4148
                     /locus_tag="ACHKCL_003734"
     CDS             3078..4148
                     /locus_tag="ACHKCL_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764618.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4176..5258
                     /locus_tag="ACHKCL_003735"
     CDS             4176..5258
                     /locus_tag="ACHKCL_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5764619.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5470..6510
                     /gene="pstS"
                     /locus_tag="ACHKCL_003736"
     CDS             5470..6510
                     /gene="pstS"
                     /locus_tag="ACHKCL_003736"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5764620.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6597..7556
                     /gene="pstC"
                     /locus_tag="ACHKCL_003737"
     CDS             6597..7556
                     /gene="pstC"
                     /locus_tag="ACHKCL_003737"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5764621.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7556..8446
                     /gene="pstA"
                     /locus_tag="ACHKCL_003738"
     CDS             7556..8446
                     /gene="pstA"
                     /locus_tag="ACHKCL_003738"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5764622.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8629..9402
                     /gene="pstB"
                     /locus_tag="ACHKCL_003739"
     CDS             8629..9402
                     /gene="pstB"
                     /locus_tag="ACHKCL_003739"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5764623.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9417..10142
                     /gene="phoU"
                     /locus_tag="ACHKCL_003740"
     CDS             9417..10142
                     /gene="phoU"
                     /locus_tag="ACHKCL_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5764624.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2728 a         2506 c         2666 g         2681 t
ORIGIN      
        1 ggttgatcca tttaataaag aggataaaag caaaccgcca ttttctgtga tacctcccgt
       61 ttctcgcatt gaaccacagc aagagaaaat attacgcatt ttaaaaacgg ctggcgagtt
      121 accaaaagat cgggagtccg tattctggtt gaacgtgaaa aacatccctc cttcaattga
      181 taaaaaaata ccaatgttct tgagatagcc ataaaaacaa gaattaagtt attctggcga
      241 ccagccgcgg taaagataat ccctgagcag gctattaaaa atgttaaatg gtcgcggcag
      301 ggtaataatc tcatcgtcga aaatccaaat ccgatccata ttaatgtaat gagtgtgacg
      361 gtggatgata aagacgtgtc gctaaatatg atccgccctt ttgaaatatt gacattacca
      421 attccggctg gcgtggcagg aaaatcgctc gtttggcgct ttattaatga ttacggtgca
      481 attagtcaac cactaaagaa aaacctttaa gcgccggata aggatatatc gatggcctct
      541 tcgcatcttt tcattacgct tgcatcgggc atatgtctgc tctgttccat atctgctttt
      601 gcccgggata gcttgttcaa ccccagatta ctggaactgg atcatcctgc ggataatatt
      661 gatattcacc agttcaaccg ttcgaatacc ttacctgcgg gaacatacaa agttgatgtg
      721 atgatcaacg gcatgctctt cgaacgccag gaagttaaat tcgcccagga taaccctgat
      781 gctgaactcc acccatgcta cgtggcgata aaaaacgtgc tggcgaccta tggtataaaa
      841 gttgatgcga taaaatctct ggcgaatgtt gatgacaaaa catgcgtaaa tccagttccg
      901 ctgatcgacg gggctacctg gttactggac gccagtaaac ttgcattgaa tattactatt
      961 ccgcaaattt atctcaacaa tgcagttaat ggttatatca gcccttcccg ttgggatcag
     1021 gggatcaatg ccatgatgat gaattatgat ttttcggcat cgcataccat ccggtcaaat
     1081 tatgacgacg acgatgacag ttattatctg aatttgcgta atggtattaa tttaggcgca
     1141 tggcgttttc gtaattacag caccctgaat tcttatgacg gtaatgtgga ctaccattcc
     1201 gtcagtaatt acattcagcg cgacatcatg gcattacgta gccagattat gattggcgat
     1261 acctggacgg caagcgatgt atttgatagt acacaggtgc gtggcgtgcg gctgtatacc
     1321 gatgacgata tgttgccctc cagccagaac ggctttgcgc cagtggtaca tgggattgcg
     1381 aaaactaacg ccacggtgat catcaaacaa aacggctacg ttatttatca atcagccgta
     1441 ccacagggcg catttgccct caccgactta aacacgacca gtagcggcgg cgatctcgat
     1501 gtcactatca aagaagaaga tggcagcgag cagcacttta ttcagccatt tacttcactg
     1561 gccattctca agcgtgaagg tcagaccgat gtagacctta gcattggaga agtgcgcgac
     1621 gaaagcggct ttacgcctga ggtcttgcag ttacaagcaa tgcacggttt ccctttggga
     1681 ataactttgt atggcggaac acaattggca aatgattacg cttctgccgc gctgggtatt
     1741 ggtaaagata tgggggcgct gggcgcgatt tcttttgacg tgactcatgc ccgctcgcag
     1801 tttgactacg acgataatga gagtggtcaa tcgtatcgtt ttctctattc caaacgtttt
     1861 gaagacacca ataccacctt tcgtctggtg ggttatcgct actctatgga ggggttctac
     1921 accctcaatg aatgggtgtc gcgacaggat aatgattctg atttctgggt aacgggcaac
     1981 cgtcgcagcc gcttcgaagg cacctggacg caatctttca cgccaggctg gggcaatatt
     2041 tatttaacat tcagtcgaca ggaatactgg cagaccgatg aggtcgaacg tttattacag
     2101 ttcggctata acaacaactg gcgaaacatc tcctggaacg tttcctggaa ctatacggac
     2161 tcgatcaagc gctcattggg caaccatcat gatgataaca atgatgattt cggcaaagaa
     2221 cagattttca tgttctcaat gtcgataccg ctatcgtgct ggatggaaga cagctacgtc
     2281 aactattcgt taacgcaaaa caaccaccat gaaagcacga tgcaggtcgg tctgaacgga
     2341 acgatgctgg aagggcgtaa cctgtcttat aacgtacagg aatcgtggat gcactctcct
     2401 gatgactcct acagcggcaa tgccggaatg acctatgacg ggacttatgg ctcggtcaat
     2461 ggtagctatt cctggagccg tgactcccaa cattttgatt atggcgccag aggcggcgtg
     2521 ctggtgcata gtgacggagt gaccttctcg caggaactgg gcgaaacggt ggcattggtc
     2581 aaagcgccgg gcgcagaagg cctgtccatt gaaaacgcca ccgggatttc taccgactgg
     2641 cgtggttata ccgtaaaaac gcagcttagc ccgtatgacg aaaaccgcgt ggcattgaac
     2701 agcgactatt tctccaaagc caatattgaa ctggaaaaca ccgtcatcaa cctggtacca
     2761 acgcgcggtg cggtggtgaa agccgaattt gtcacccatg tcggttatcg cgtgctattt
     2821 aacgtccgcc aggtcaacgg taaaccaata atgtttggcg cgatggcaac cgcctctctc
     2881 gaaacgggca cagtcaccgg gattgtcggt gataacggcg aactgtatct ctccgggatg
     2941 cctgaaaaag gcgagttttt attgagttgg ggacaagctg cggatgaaaa atgtaaggcg
     3001 gcctatcaca tcacccataa acctgatgat accagcctgg ttcaaatgga tgcgatttgt
     3061 cgctaaagga agcatgaatg aaaaaaatat tatcagggtt gattctgctg ctttgctgtc
     3121 cttatggttt cgccgctaat ggtgatggcg caacgcacat gtcaaattta tcatttggtc
     3181 cgctgacggt ggcagcggcg aataaccact ccggatacaa tattttcgag gcactgagca
     3241 acacgactgg aacatacccg gtgcgctgtc actgtgatga cacgcatggc ggaccgggcc
     3301 aacaaacagc attttttcct atcttctaca cgggagatgc cgcaccgggg cttgtgcttg
     3361 agcgcactct taatgggtta aattactatg ctctgaatga ttatttatcg gtcggcgtga
     3421 cgatttttat tattaataac caatatgcgg ccattccttt tgaacactta tccaaccaat
     3481 ccacctcacc gcaacatacc tgtggagcag gtaataatgg gagcactgta aatctggatt
     3541 cagggcgctc ggcaaaatta tctttctatg ttcggcattc tattactggc acggtgacaa
     3601 tacccacaac ggaagtcgcc tggttgtacg cgggcatgtc cgatcatttt cccaaaacga
     3661 cccccgtttc taaagtgaca attcgcggac aactaacggc cccgcagaac tgtgagttaa
     3721 cgccaaatca gagcatcgat gtcgattttc aaaaaattaa tagcgctgag ttctcctcaa
     3781 cggcgggttc aattattgca gaaagaaaga ttaaaaccga agtcacagta tcctgtaccg
     3841 ggatggaaga cgtaaggtcc acggaggtgg tgagtgcgtc gatgattgcg gcaaacagaa
     3901 gtgccgatgc caccatgatc gtgacgagta atccggatgt gggaattaag atttttgata
     3961 agaacgaccg tccagtgaat gtggatgggg gcaacttacc cgctgatatg ggtgctatta
     4021 gtcgattagg aaaaaccgat ggtagcgtaa cgttttattc agcgcccgcc agtctgacgg
     4081 gcgcaaaacc agcgcctgat aatggattta ccgctacagc cacgctggtt attgaattta
     4141 ctaactaatc ttgtgttagt aaaaggaata atttcatgaa aaaatggcat tatatatttt
     4201 gcataattct ctttcattta gggttaccgt gcgggtatgc ggcaaatgat ggcacgtgtg
     4261 caacaagagg cggcacacat acattaagcc ttaattttcc tctgacaacg gtcagtgcag
     4321 caaacaatgt gcctggaaat acattaatag atattgctaa tgcaacatct tctgaaaatt
     4381 atagcgttct gtgtaactgt gattcaaaac atagcaatgg cgcttatcac gaaatatatt
     4441 ataccgcaga ccctgctccc ggtatggttt atagcaccac cgcaagtggt cttgcttttt
     4501 actatcttaa cgaatatgtc gatgtgggaa caaaaatatc tgtgctaaat gcggggtata
     4561 cggcagttcc ttttgaacat gtttccaacc aggcaactac aacagatcac acttgtcagg
     4621 gaaacaaaac tacagcggtt ggcgtgagcc tgaaaactgg agcagatgcg aagatttcat
     4681 ttcgtattaa acgttcaata aatggaacgg tagtaatacc tatcaccgat attgcattgc
     4741 tgtatgccaa catatccagc accacgaccc gtggtgaggc gattgcaaaa gttcgaattt
     4801 caggcagttt gaccgcacca cagtcttgtc agataaatgc aggacaggtg atttattttg
     4861 attttgatac tattcctgcg tccgaatttt catctaccgc cgggcaagcc attacttcac
     4921 gaaaaatcac taaaacagtg agtattgagt gtacggggat ggggtatgag cgtacgcaga
     4981 aagtcgatgc ttcttttacg gggacgaacc gaagcagtga cgatacgatg gtggcgacag
     5041 acaatgctga tgtcgggatc aaaatttaca ataaatcgaa tgctgaagtt agcgtcaaca
     5101 acggcaagtt acccgcagac atgggcaaca cgaccatttt tggtcgtaaa aatggttcgg
     5161 taactttttc ggcagcacct gccagcttta ccggtgcccg gcctcagccc ggcgttttta
     5221 acgctaccgc gaccttaacc attgaatttg taaactaacc ttttgcggct gttttatgac
     5281 agccgttcat atttcctctc ctttcatgaa aaaatgctga tatttttccc cttcaatatc
     5341 aataagtaat aactttcctt tgggtgtcat caaactgtca cattccttac atataactgt
     5401 cacctgtttg tcctattttg cttctcgtag ccaacaaaca atgctttatg aatcctccca
     5461 ggagacatta tgaaagttat gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg
     5521 atgagtgctt tctctgtgtt tgcagaagca agcctgacag gtgcaggtgc aaccttccct
     5581 gcgccggtgt atgccaaatg ggctgacact taccagaaag aaaccggtaa taaagttaat
     5641 taccagggta tcggttcttc cggtggcgta aaacagatta tcgccaatac cgttgatttt
     5701 ggtgcttctg acgcgccgct gtctgacgaa aaactggctc aggaaggtct gttccagttc
     5761 ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc caggtctgaa gtctggtgag
     5821 ctggtgctgg atggtaaaac cctcggcgat atctacctgg gcaaaatcaa gaagtgggat
     5881 gatgaagcca tcgccaaatt gaatccgggt ctgaaactgc cttcacagaa cattgctgta
     5941 gtgcgccgcg cagatggctc cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg
     6001 aatgaagagt ggaaaaacaa cgttggtact ggctctaccg taaaatggcc gatcggtctg
     6061 ggtggtaaag gtaacgacgg tatcgctgcg ttcgttcagc gtctgccggg tgcaattggt
     6121 tacgttgaat atgcttatgc gaagcaaaac aacctggcgt acaccaaact gatctccgct
     6181 gatggtaaac ctgttttgcc gaccgaagaa aacttcgcta atgcagcaaa aggtgcagac
     6241 tggagcaaaa ccttcgctca ggatctgacc aaccagaaag gcgaagatgc gtggcctatt
     6301 acctctacca cgttcattct gatccacaaa gatcagaaga aaccagaaca aggcacagaa
     6361 gtgctgaaat tcttcgactg ggcgtacaaa accggggcta aacaggcgaa cgacctggat
     6421 tacgccagcc tgccggatag cgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt
     6481 aaagacagta gcggcaagcc gctgtactaa taaaactcca ggccggataa ggcattaaac
     6541 ggcttatccg gcattacaaa atgactttgt aaacgcgttt aactgaagag taacttatgg
     6601 ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc
     6661 tggtaaaact ggcggcgctg attgtgctat tgatgttggg tggcattatt gtctctctga
     6721 tcatctcctc atggccgagc attcagaaat ttggtctggc tttcctgtgg accaaagagt
     6781 gggatgcgcc gaacgatatc tacggggcgc tggtgccgat ctacggtacg ttggtgactt
     6841 cgtttatcgc gctgctgatc gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg
     6901 agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc cattgagctg ctggcagcca
     6961 ttccaagtat cgtttacggc atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt
     7021 acttccagga gccggtcggc aatatcatgt cgaatatccc gattgtcggc gcgctgttct
     7081 ctggcccggc atttggtatc ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta
     7141 ttccgtacat tgcggcggta atgcgcgatg tgttcgaaca aaccccggtg atgatgaaag
     7201 agtcggccta cggtattggc tgcaccacct gggaggttat ctggcgtatc gttcttccgt
     7261 tcaccaaaaa tggtgttatc ggcggcatta tgctggggct gggccgcgcg cttggtgaaa
     7321 ccatggcggt gacctttatt atcggtaaca cctaccagct cgacagcgcc tcgctgtaca
     7381 tgccgggcaa cagtatcacc tctgcgctgg cgaacgaatt tgccgaagcg gaatccggtc
     7441 tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc
     7501 tcgccgcatc gaagtttatg attatgcgcc tggctaagaa tgagggggct cgctaatggc
     7561 tatggttgaa atgcaaacca ctgcggcgct ggcggaatcc cgccgcaaaa tgcaggcgcg
     7621 tcgccgcctc aaaaaccgta ttgcactgac gctctcaatg gcgacgatgg ccttcggtct
     7681 gttctggctg atctggattt taatgtccac catcacccgc ggtatcgacg gtatgtcgct
     7741 ggcgctgttc actgaaatga cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc
     7801 tctggcaggt agcgggctgt taattttgtg ggccacggta ttcggtacgc cgctgggcat
     7861 tatggcgggg atttatctgg ctgaatatgg tcgtaaatcc tggctggcag aagtgattcg
     7921 cttcattaac gacattctgc tctctgcgcc gtcgattgtg gttggtctgt ttgtttacac
     7981 catcgtggtg gcgcagatgg agcacttctc cggttgggcg ggtgtgattg cgctggcgtt
     8041 gttgcaggtg ccgattgtta tccgcaccac cgagaacatg ctgaaactgg tgccggacag
     8101 cctgcgtgaa gcggcttatg cgctgggtac accgaagtgg aagatgatct ccgcgattac
     8161 gctgaaagcg tcggtgtccg ggatcatgac cggtatcctg ctggcgattg cccgtattgc
     8221 tggtgaaacc gcgccgctgc tgtttaccgc gctctccaac cagttctgga gcacggacat
     8281 gatgcagccg atcgccaacc tgccggtgac gatctttaag tttgcgatga gcccgtttgc
     8341 ggaatggcag caactggcct gggccggggt attgatcatt accctgtgcg tactgctgct
     8401 gaacattctg gcgcgcgttg ttttcgcgaa gaataaacac ggttgatatt gctgacacgg
     8461 ttttcccctc accctaaccc tctccccaga ggggcgaggg gaccgaccga gcgccttttt
     8521 gactctgtac acggttaaca ctttgccgga tgcggcgtga acgcctgatc cggcctacgg
     8581 taagcctgat tcgcgaagtg catcaggcac gatgaggaaa agattgcaat gagtatggtt
     8641 gaaactgccc cgagtaaaat tcaggttcgt aatttgaact tctactacgg caaattccat
     8701 gccctgaaaa acatcaacct ggatatcgcc aaaaaccagg taacggcgtt tatcgggccg
     8761 tccggctgcg gtaaatcgac gctgctgcgt accttcaaca aaatgtttga actgtacccg
     8821 gaacagcgtg cggaaggtga aattctgctc gatggcgaca acatcctgac caactctcag
     8881 gatatcgccc tgctgcgtgc gaaagtgggc atggtgttcc agaaaccgac gccgttcccg
     8941 atgtccatct acgacaacat cgcctttggc gttcgtctgt ttgagaagct ctctcgtgcc
     9001 gacatggacg agcgcgtgca gtgggcattg accaaagccg cattgtggaa cgaaaccaaa
     9061 gataaattgc atcagagcgg ttactctctc tctggtggtc agcaacagcg tctgtgcatt
     9121 gcgcgtggta tcgccattcg cccggaagtg ctgctgctcg acgaaccatg ttcggcgctc
     9181 gaccccatct ccaccgggcg tattgaagag ctgatcaccg aactgaagca ggattacacc
     9241 gtggtgatcg tcacccacaa catgcagcag gctgcgcgtt gttccgacca cacggcgttt
     9301 atgtacctgg gcgaattgat tgagttcagc aacacggacg atctgttcac caagccagcg
     9361 aagaaacaaa cagaagacta catcaccggt cgttacggtt gattcaggag tgcgttatgg
     9421 acagtctcaa tcttaataaa catatttccg gccagttcaa cgccgaactg gaaagtatcc
     9481 gcacgcaggt gatgaccatg ggcggcatgg tggagcagca gctttctgat gcaatcaccg
     9541 cgatgcataa ccaggacagc gatctggcga agcgcgtgat cgaaggcgac aagaacgtca
     9601 acatgatgga agtggcgatc gatgaagcct gcgtgcgcat tattgccaaa cgccagccga
     9661 cggcgagcga cctgcgattg gttatggtga tcagtaaaac cattgccgaa ctggagcgta
     9721 ttggcgacgt ggcggacaaa atctgccgta ctgcgctgga gaaattctcc cagcagcatc
     9781 agccgttgct ggtaagtttg gagtcgctgg gccgtcatac tatccagatg ctgcacgacg
     9841 tgctggacgc gttcgcgcgg atggacattg acgaagcggt acgtatttat cgtgaagata
     9901 aaaaagtcga tcaggagtac gaaggtattg ttcgtcaact gatgacctac atgatggaag
     9961 attcgcgcac cattccgagt gtacttaccg cgctgttctg tgcgcgttct atcgaacgta
    10021 ttggcgaccg ctgccagaat atttgtgagt ttatcttcta ctacgtgaag ggtcaggatt
    10081 tccgtcatgt aggcggtgat gagctggata aactgctggc gggaaaagac cccaaagagt
    10141 agtgctaact gtaggccaga taaagctttt tgccgcatct ggcaatcagc ccggtcatac
    10201 atagttaccg ggctgataca ctttctcact gaaatattgc cacacaaact cacctcgaaa
    10261 ctctccccag tacattgaat atacattatt tcatttgaat tattttactt ttaatgtagt
    10321 gcctcgctat aaaaacatat ttgttattct tttcatttat atcgctacct gcacagagaa
    10381 tggaaaagaa aacaaaaaac agggtaaaag ccctatatcg agacataaaa aatgagcgat
    10441 tttttacagt attcataggg ggtttagtca taatcgtagc aaaccaacac ccaggtatgc
    10501 tttttaagac aaatcggcaa tgtattatgc tcgggcgaat aaatattgtg ttcaggagaa
    10561 atgattatgg ctatatcatc a
//
LOCUS       DAZFKY010000163        10536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFKY010000163 DAZFKY010000000
VERSION     DAZFKY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            134..1490
                     /gene="dcuC"
                     /locus_tag="ACHKCL_003741"
                     /pseudo
     CDS             134..1490
                     /gene="dcuC"
                     /locus_tag="ACHKCL_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1533..2030)
                     /gene="sspB"
                     /locus_tag="ACHKCL_003742"
     CDS             complement(1533..2030)
                     /gene="sspB"
                     /locus_tag="ACHKCL_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5764625.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2036..2674)
                     /gene="sspA"
                     /locus_tag="ACHKCL_003743"
     CDS             complement(2036..2674)
                     /gene="sspA"
                     /locus_tag="ACHKCL_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5764626.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3069..3461)
                     /gene="rpsI"
                     /locus_tag="ACHKCL_003744"
     CDS             complement(3069..3461)
                     /gene="rpsI"
                     /locus_tag="ACHKCL_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5764627.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3477..3905)
                     /gene="rplM"
                     /locus_tag="ACHKCL_003745"
     CDS             complement(3477..3905)
                     /gene="rplM"
                     /locus_tag="ACHKCL_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5764628.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4124..5251)
                     /gene="zapE"
                     /locus_tag="ACHKCL_003746"
     CDS             complement(4124..5251)
                     /gene="zapE"
                     /locus_tag="ACHKCL_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5764629.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5445..5843
                     /gene="zapG"
                     /locus_tag="ACHKCL_003747"
     CDS             5445..5843
                     /gene="zapG"
                     /locus_tag="ACHKCL_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5764630.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5997..7364
                     /gene="degQ"
                     /locus_tag="ACHKCL_003748"
     CDS             5997..7364
                     /gene="degQ"
                     /locus_tag="ACHKCL_003748"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5764631.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7454..8521
                     /gene="degS"
                     /locus_tag="ACHKCL_003749"
     CDS             7454..8521
                     /gene="degS"
                     /locus_tag="ACHKCL_003749"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5764632.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8585..9523)
                     /gene="mdh"
                     /locus_tag="ACHKCL_003750"
     CDS             complement(8585..9523)
                     /gene="mdh"
                     /locus_tag="ACHKCL_003750"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5764633.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9958..10428
                     /gene="argR"
                     /locus_tag="ACHKCL_003751"
     CDS             9958..10428
                     /gene="argR"
                     /locus_tag="ACHKCL_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5764634.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2595 a         2684 c         2625 g         2632 t
ORIGIN      
        1 atgaaaaatc acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca
       61 aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccctt ttcttttgac
      121 acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta cgatgggcta
      181 tttgatcctg aaaaactaca aacctcaggt ggtgctggct gcaggtatct tcctgatgat
      241 gtgcggtgtc tggttagggt taggtggtgt actcgatccc gccaaaagca gcggctactt
      301 gatcgtcgat atttataatg aaatcctgcg catgctgtcc aaccgcattg ccggattggg
      361 gctgtcgatt atggcggtgg gcggttatgc ccgctacatg gagcgcacag gagccagtcg
      421 cgcgatggtg agcttgttaa gccgcccgtt aaaactcatt cgctcgccgt atattattct
      481 gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt attaccagcg cctccggcct
      541 gggtatgttg ctgatggtca ccttatttcc gacactggtg agtctgggag taagccgtct
      601 ctctgcggtg gcggttatcg caaccacaat gtccattgag tgggggattc tggaaacgaa
      661 ctccattttt gcagcccagg tagcgggaat gaaaattgcc acttacttct tccactacca
      721 gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt tcgtgcaacg
      781 cgcttttgac aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa aagctctcga
      841 taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatcc tgatgctcgg
      901 ctcgctattc cttgcccaca tcgggctgat gcagtcagaa ctgcatctgg tggtggtgat
      961 gttactgagt ttgacgatgt ttgttgagtt cttccgcaag cataacttgc gcgaaacaat
     1021 ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgccaacg tggtaacgct
     1081 ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg tcgatgcggt
     1141 tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta ttatggcgct
     1201 ggtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta tgtcatttgc
     1261 cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa tgattatgcc
     1321 gatgcatttt gccacgacgc tagcgcgcgg tttcgccgat tactgcggtg gtggtcgtta
     1381 cgtcaggaat tgcaggcgtt tcgccttttg cggtggtgaa gcggacagcg atccccatgg
     1441 cagtcggttt cgtggtgaat atgattgcca caatcacgct attttattaa gtcattaaaa
     1501 gacaaaacag gccgccaggg cctgtttttg tattacttca caacgcgtaa tgccggtcga
     1561 ccaccgcgtg gtggctgcgg tggttcatcg tcaggatgag tgtcatcatc gtgatctggc
     1621 ttgtcgccat caataaccga cataacggtt tcgttgtctg ccgatgcctc ttcatcattc
     1681 atgatgctgg tatcttcatc gtaggcagct tcaggctcaa acatcgtgcc tgcgccattt
     1741 tcacgggcgt agatagccag cacggcagcc agcggcacag aaacctgacg cggaatgcca
     1801 ccaaagcgcg cgttaaagcg cacctcatca ttcgccagtt ccagattgcc gacagcacgc
     1861 ggcgcaatgt tgagtacgat ttgcccgtca cgcgcatatt ccataggaac ctgcacgcca
     1921 gggagcgtca catccaccac caggtgcggc gtgagctggt tatccagcaa ccactcatag
     1981 aatgcacgca gcagataggg acgacgtggt gttagctgtg acaaatccat acagattaac
     2041 tccggcccag acgcatttca cgttctgctt cagttaaaga agcaaggaaa gagtcacgct
     2101 caaagacgcg ggtcatatag cctttcagct ctttcgcacc cgggccgctg aactcgatac
     2161 ccagttgcgg cagacgccac agcagcggag caaggtagca atcgaccagg ctgaactcat
     2221 cgctcaggaa gtacggcttc tgaccgaaga ccggcgcaat cgccagcagt tcttcgcgca
     2281 gttgcttacg tgcggcatct gcttcagaag ctgaaccgtt gatgatggtg ttcatcagcg
     2341 tgtaccagtc tttttcgatg cgatgcatgt acaaacggct ttcaccgcga gctaccgggt
     2401 aaacaggcat cagtggcgga tgcgggaaac gctcatccag atattccata atgatgcgag
     2461 attcccacag ggtcagctca cgatccacca gggtcggaac gctctgattc gggttgaggt
     2521 caatcagatc ctgaggcgga ttgtcctttt ccacgtgttc gatctcgaaa cttacacctt
     2581 tctcagccag cacaatgcgg acctgatggc tatagatgtc agtaggaccg gaaaacagcg
     2641 tcattaccga acgtttgttg gcagcgacag ccatgaaaac ctccaggtat agtcagaatt
     2701 tttactgcta ccagccacca ggtggccagt cagaagttgt gttacccagt aaggaacgac
     2761 tctctttgtt cgaaaatcaa acaaaaaatg agcaataccc gacatttggg cagaaaattg
     2821 gatgatagtt taccagattt tgcgaccttt gtggtgagtc gatgccggaa atggggaaaa
     2881 agagatgcgc tttagtctga aatagttgac ttagtccctt attggcgatg tggtttttgt
     2941 tttgcctgtc tgtcagacgg cagcaaaaag caactttcca ttttttacgc tgattcagaa
     3001 tttaaccata aaaaaacccg ccgaagcggg ttttttcgca aattgttttc tgccggagca
     3061 gaagccaatt aacgtttgga gaactgcgga cgacgacgtg ctttacgcag accgactttc
     3121 ttacgttcaa cctgacgagc gtcacgagta acgaagccag ctttacgcag ttcagaacgc
     3181 agggactcgt cgtattccat cagagcgcgg gtgataccgt gacggatcgc accagcctga
     3241 ccagagatac caccaccttt aacggtgatg tacaggtcca gtttctcaac catatcgacc
     3301 agttccagcg gctgacgaac taccatgcga gcagtttcac gaccgaagta ctgttccaga
     3361 gaacgttggt tgattacgat tttaccgttg cccggtttga tgaaaacgcg agctgcggaa
     3421 cttttgcggc gaccagtgcc gtagtattga ttttcagcca ttgcctataa tcccgattag
     3481 atgtcaagaa cttgcggttg ctgtgccgcg tggttgtgct cgttacccgc gtaaactttc
     3541 agtttacgga acatagcacg acccagcggg ccttttggca acatgccttt aaccgcgatt
     3601 tcaatcacac gctcaggacg gcgagcaatc atctcttcaa aggtcgcttg tttgatacca
     3661 ccgatgtggc cggtgtggtg atagtacact ttgtcagtac gcttgttgcc ggttacagca
     3721 actttgtcag cgttcagaac gatgatgtaa tcaccggtat ctacgtgcgg agtgtattcc
     3781 gctttgtgct taccgcgcag gcgacgagcc agttcagtag ccagacggcc cagagtttta
     3841 ccggtcgcgt caacaacata ccagtcgcgt tttacggttt ctggtttagc tgtaaaagtt
     3901 ttcattaaaa gcttacccaa taaatagtta cacgttggtg aacacccaaa cgtcttcaat
     3961 tgttgaggtt cacacgacaa agtccggcaa acctacccct tcgaatagcc tatgccagca
     4021 cacaaaaagt tttgggaaaa aaactttctt gtaacgtggg gtcgcaggat tatagagaag
     4081 tcggggtcaa agatcgaccc ctttttgtga tttgtggcag gttttaaccc gctaaatgct
     4141 cgcgcttcag atactcttcg ctttgcatct cttgcagacg tgacaggcaa cgctggaact
     4201 caaacttcag ccgatcgccc tgataaattt catacagcgg cacttctgca ctcaccacta
     4261 atttgacatg gcgctcgtaa aactcatcca ccagcgcaat aaagcgccgc gcttcgctct
     4321 ccatcaaccg cgtcataact ggtacatcaa acaacatgac cgtatgaaag agacgtgaga
     4381 gcgcaatata gtcatgctga ctgcgggcgt cgacgcacag cgtagtaaaa gagaccgcca
     4441 gcgtctggtt ctcgacgccc attgtcgcta atggccgatg gttgatttct aacgtcggtg
     4501 aattttctcg tttccccccc gccagcgcca accatagttt atccatttgc gcccgggttt
     4561 catcgtggag tggcgaaagc cacagatgcg cctgagtgag tgtacgcaga cgataatcaa
     4621 caccagcgtc cacgttcatt acatcacaat gctgtttaat ggcatcgatt gcaggcagaa
     4681 aacgcgcacg ttgcaagcca tttcgataaa gttcgtccgg cggaatattt gacgtcgcta
     4741 ccagggtaat accgcgggcg aacagggctt tcatcaggcc gccaagtagc atggcatcgg
     4801 taatatcaga gacaaaaaat tcgtcaaaac agagcacgtc agtttctgct ttaaaacgat
     4861 cagcaataat ttccagcgga tcggtctgcc cctgtaaggc agttagctct tcatgcaccc
     4921 gcagcataaa acggtgaaag tgcaggcgct gtttccgctc tcccggcagg ctttgataga
     4981 aaaggtccat cagccaggtt ttcccgcgtc ctacaccgcc ccacatatat aagccacgca
     5041 ctggcgtatg ctttgtgtct tcccgtttac cccacagctt accgacccgc gccattagcc
     5101 cactcgtcct gggggctggt ggcgtgctat tgatgagttc ctgataaata atttccaagc
     5161 ggctgacggc ctctttttga acgtcgtcgg gttgatggct gccttcattg agcgccttca
     5221 ggtattgcga tgttggagta acgctttgca tgatcttatt gttattcctt gaataatcgg
     5281 tgcgccgttg ttcacggttg acgaaaaaaa ggccgttcta cactacgcga tatgcagtcg
     5341 ggattccact tctgtggaat taacggttat agtggcataa tcagccgcag gcatggagcc
     5401 tgaagccaac accctacgga aacaaaagac aacgggagat gttcatgacc tgggaatatg
     5461 cgctaattgg gttagtcgtc ggcatcatta ttggtgctgt ggccatgcgt tttggtaatc
     5521 gtaaactacg ccagcaacag gcgttgcagt acgaactgga aaagaataaa gctgaactgg
     5581 acgagtatcg cgaagagctg gttagccact ttgcccgcag cgcggaatta ctggatacca
     5641 tggcgcacga ctatcgccag ctgtatcagc acatggcgaa aagctccagc agcctgctgc
     5701 cggaactgtc tgctgaagca aacccgttcc gtaatcgtct ggcagagtct gaagccagca
     5761 acgatcaggc accggtgcag atgcctcgcg actattctga aggcgcatcc ggcctgctgc
     5821 gtactggcgc gaagcgcgac taatttattt ttcgggcgca gccattgcgc cctcctcttc
     5881 tctccctccc cgactatcat ttaatctggt gtctcattgt tagccgtctg aaaattcaat
     5941 aacatcaaac tgttttgaat ctcttttctt atcattcagg tacgagagca ggaataatga
     6001 aaaaacaaac ccagctgttg agtgcattag cgttaagtgt cgggttaact ctctcggcgt
     6061 catttcaggc cgttgcgtcg atcccaggcc aggttgccga tcaggccccc ctccccagtc
     6121 tggctccaat gctggaaaaa gtgcttccgg cagtggtgag cgtacgggtg gaaggaacgg
     6181 ccagtcaggg acagaaaatc ccggaagaat tcaaaaagtt ttttggtgat gatttaccgg
     6241 atcaacctgc acaacccttc gaaggtttag gctccggtgt catcatcaac gccagtaaag
     6301 gctatgtgct gaccaacaac catgtgatta atcaggcaca gaaaatcagt attcagctca
     6361 atgatgggcg cgagtttgat gcaaaactga ttggtagcga tgaccagagc gatatcgccc
     6421 tgttgcaaat tcaaaacccg agcaaattaa cgcaaatcgc tattgccgac tccgataaat
     6481 tgcgcgtcgg tgattttgcc gtggcggtcg gtaacccatt tggccttggg caaaccgcca
     6541 cctctggcat tgtttccgca ttaggacgca gcgggttgaa tcttgaaggt ctggaaaact
     6601 ttatccagac agatgcttcc attaaccgcg gtaactccgg cggtgcactg ttaaacctta
     6661 acggtgagtt aattggcatc aacactgcaa tccttgcgcc tggcggcggg agcgtcggaa
     6721 ttggatttgc catccccagt aatatggcgc gaacactggc gcagcagctt atcgactttg
     6781 gtgaaatcaa acgcggtttg ttaggcatca aaggcaccga gatgagtgcc gatatcgcca
     6841 aagccttcaa ccttgacgtg cagcgtggcg cgtttgtcag cgaagtgttg ccaggttctg
     6901 gctcggcgaa agcgggcgtc aaaacgggcg atattattac cagcctcaac ggcaaaccgc
     6961 tgaatagctt tgctgagttg cgctctcgta tcgcgaccac cgagccgggc acgaaagtga
     7021 agcttggcct gctgcgtaac ggcaaaccac tggaagtaga agtgacgctc gataccagca
     7081 cctcttcgtc ggccagcgct gaaatgatca cgccagcgct ggaaggtgca acgttgagcg
     7141 atggtcagct aaaagatggc ggcaaaggta ttaaaatcga tgaagttgtc aaaggaagcc
     7201 cagctgctca ggctggcttg caaaaagacg atgtgatcat tggcgtcaac cgcgatcggg
     7261 tgaactcgat tgctgaaatg cgtaaagtgc tggcggcaaa accggccatc atcgccctgc
     7321 aaattgtacg cggcaatgaa agcatctatc tgctgatgcg ttaatgtcgt aaaccgggca
     7381 tcaggctgac gtgtgatgtc cggttaactc gtggtatgct gctgccgttc ccttttttaa
     7441 tgacgcctcc atcatgtttg tgaagctctt acgttccgtt gcgattggat taattgtcgg
     7501 cgctattctg ctggttgcca tgccttcgct gcgcagcctt aacccgcttt ccactccgca
     7561 atttgacagt accgatgaga cgcctgccag ttataatctg gcggttcgcc gcgccgcgcc
     7621 agcggtggtt aacgtttaca accgtggttt gaacaccaac tctcacaacc agcttgagat
     7681 ccgcaccctg ggatccggtg taatcatgga tcaacgcggt tatatcatca ccaataaaca
     7741 cgtcatcaac gacgccgatc agatcatcgt cgccttacag gatggacgtg tatttgaagc
     7801 attgctggtg ggatctgact ctctaaccga tctggcggta cttaaaatta atgccactgg
     7861 cggtttacct accattccaa ttaatgcacg tcgcgtaccg cacattggcg acgtggtact
     7921 ggcgatcggt aacccgtaca acctcgggca gaccattacc caggggatta ttagtgctac
     7981 gggtcgaatc ggtctgaacc cgaccgggcg gcaaaacttc ctacaaaccg atgcttccat
     8041 taaccacggt aactctggcg gcgcgctggt gaactcgctg ggcgaactga tgggcattaa
     8101 cacgctgtcg tttgataaga gtaacgatgg cgaaacgccg gaaggtatcg gctttgcgat
     8161 tcctttccag ttagcaacca aaattatgga taagctgatc cgcgatggtc gcgtgatccg
     8221 cggctacatt ggtatcggcg gacgcgagat cgcaccactg cacgcgcagg gcggtggtat
     8281 agatcaactg caagggatcg tggttaatga agtgtcacct gacggcccgg cggcgaatgc
     8341 gggtattcag gtcaacgatc tgattatttc ggtggataac aaaccggcca tctctgctct
     8401 ggagacgatg gatcaggtgg cagaaattcg ccctggttcg gtgatcccgg ttgtagtgat
     8461 gcgtgatgat aagcagttaa cgctgcaggt caccattcag gaatatccgg caaccaatta
     8521 aggccagcgt gaaagacaaa aaaaccggag tctgtgctcc ggttttttat tatccgctaa
     8581 tcaattactt attaacgaac tcttcgccca gggcgatatc tttcttgagc gtatccagca
     8641 taccttccag cgcgttctgt tcaaatgcgc tcagggtacc gatagattta cgctcttcca
     8701 cgccgttttt acccagcaga agcggctgag agaagaaacg ggcgtactga ccgtcgcctt
     8761 caacgtaggc acattcgaca acgccttgtt cgccctgcag tgcgcgaacc agagacaggc
     8821 caaaacgtgc agcagcctgg cccatagaca gggttgccga cccgccaccg gctttcgctt
     8881 caaccacctc agtacccgcg ttctggatac gtttggtcag atcagccact tcctgctcgg
     8941 taaaactaac gccaggaacc tgtgacaaca gcggcagaat ggtaacacca gagtgaccgc
     9001 caataaccgg cacttcaact tcgcctggct gtttgccttt cagttccgca acaaaggtgt
     9061 tggaacgaat gatatccagc gtggtaacgc cgaacagttt gtttttgtca taaacaccgg
     9121 cttttttcag cacttcagca gcaatcgcaa ctgtggtgtt aaccgggtta gtgataatac
     9181 caatgcacgc tttcgggcag gttttcgcaa cttgctgtac caggtttttc acgatgccgg
     9241 cgttaacatt aaacaggtcg gaacgatcca tacccggttt acgcgctaca cctgcagaga
     9301 taagaacgac atctgcgcct tccagcgccg gagtcgcatc ttcaccagaa aaacctttga
     9361 ttttcacagc agtagggata tggctcagat cgacggccac accgggagtc actggagcga
     9421 tatcatacag agagagttct gaacctgaag gcagttgggt ttttaacagt agtgcaagcg
     9481 cctggccaat accgccagca gcgccgagga ctgcgacttt catcctaaac tccttattat
     9541 attgataaac taagatatgt tgctccgctg ccgcgacctt aatccacaaa attgccgttt
     9601 acaatgacca catctcaaga atgtgtagtc acgcaagttt agcgtttatg catttaattg
     9661 ccgtaatcag gaacctaagt tacgtaatta acaatcgctt atgcattagc gcaacattcc
     9721 aacaggtggt gacaatatac cctaccgttc agccaaaaca acatcaattt gataacaatt
     9781 aatttacttt taagcagaat ttgcatgccg tgacgcaggc atatttctca ataacgaaat
     9841 ttgataaaat cccgctcttt cataacatta tttcagcctt cttcagggct gactgtttgc
     9901 ataaaaattc atctgtatgc acaataatgt tgtatcaacc accatatcgg gtgacttatg
     9961 cgaagctcgg ctaagcaaga agaactagtt aaagcattta aagcattact taaagaagag
    10021 aaatttagct cccagggcga aatcgtcgcc gcgttgcagg agcaaggctt tgacaatatt
    10081 aatcagtcta aagtctcgcg gatgttgacc aagtttggtg ctgtacgtac acgcaatgcc
    10141 aaaatggaaa tggtttactg cctgccagct gaactgggtg taccaaccac ctccagtcca
    10201 ttgaaaaatc tggtgctgga tatcgactac aacgatgcag ttgtcgtgat tcataccagc
    10261 cctggcgcgg cgcagttaat tgctcgcctg ctggactcac tgggcaaagc agaaggtatt
    10321 ctgggcacca tcgctggcga tgacaccatc tttaccaccc ctgctaacgg tttcaccgtc
    10381 aaagacctgt atgaagcgat tttagagctg ttcgaccagg agctttaatc tctgccccgt
    10441 cgcttctggc ggcggggaaa atgttgctta tccctctcaa ccccctgctt tcccctgcga
    10501 ttaatttaac gaatagtgca ttttactgcg acatgt
//
LOCUS       DAZFKY010000164        10358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFKY010000164 DAZFKY010000000
VERSION     DAZFKY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHKCL_003752"
     CDS             complement(259..2832)
                     /gene="clpB"
                     /locus_tag="ACHKCL_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5764635.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHKCL_003753"
     CDS             complement(2962..3693)
                     /gene="yfiH"
                     /locus_tag="ACHKCL_003753"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5764636.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHKCL_003754"
     CDS             complement(3690..4670)
                     /gene="rluD"
                     /locus_tag="ACHKCL_003754"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5764637.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4805..5542
                     /gene="bamD"
                     /locus_tag="ACHKCL_003755"
     CDS             4805..5542
                     /gene="bamD"
                     /locus_tag="ACHKCL_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5764638.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5813..6154
                     /gene="raiA"
                     /locus_tag="ACHKCL_003756"
     CDS             5813..6154
                     /gene="raiA"
                     /locus_tag="ACHKCL_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5764639.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6257..6382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6258..6305
                     /gene="pheL"
                     /locus_tag="ACHKCL_003757"
     CDS             6258..6305
                     /gene="pheL"
                     /locus_tag="ACHKCL_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5764640.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6404..7564
                     /gene="pheA"
                     /locus_tag="ACHKCL_003758"
     CDS             6404..7564
                     /gene="pheA"
                     /locus_tag="ACHKCL_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5764641.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHKCL_003759"
     CDS             complement(7607..8728)
                     /gene="tyrA"
                     /locus_tag="ACHKCL_003759"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5764642.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHKCL_003760"
     CDS             complement(8739..9809)
                     /gene="aroF"
                     /locus_tag="ACHKCL_003760"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5764643.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10019..>10358
                     /gene="yfiL"
                     /locus_tag="ACHKCL_003761"
     CDS             10019..>10358
                     /gene="yfiL"
                     /locus_tag="ACHKCL_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5764644.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADE"
BASE COUNT         2641 a         2739 c         2432 g         2546 t
ORIGIN      
        1 atacgctttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
       61 gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      121 tagggagtta ttccggcctg acaagcgaaa aatataaaaa ctttatcgtt cgctcatttt
      181 tcaggcaaaa catcttaaat atagtctttt ccgtctaact tatagacaaa aacgagcccc
      241 gaagggctcg ttttatcatt actggacggc gacaatccgg tcttcattaa cttccaggcg
      301 aataacttta cccggaacca attcaccaga cagtatttgc tgtgccaacg ggttttcgat
      361 ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt aaccgttctc
      421 gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg
      481 tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt cgcccagcgg
      541 atggaagacc accacttcat cgatacggtt aatgaactcc ggacggaagt tatggcttac
      601 cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat
      661 cagatcggaa ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct
      721 cccttgcccg tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc
      781 cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac gacgcaccgc
      841 ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac caaccagacg
      901 agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct
      961 atcaaacata aagttcgcca gcgccttaca aagctctgtt ttccccacac cagttgggcc
     1021 gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg
     1081 aatagcgtta gataccgcat caaccgcttc gttctgacca attacgcgat ggtgcagttc
     1141 ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc
     1201 cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac gcaacagacg
     1261 catagttttg ccttcgagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt
     1321 gccgtattgc agttcagaca tccgcgccaa gtcacccaca cggcgagcct gttcaatagc
     1381 gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgccttctc
     1441 tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt
     1501 gagcatatcc agacgttttt tactggcttc atcagactct ttcattaacg cctgttgttc
     1561 cagtttgagc tggatgatac ggcgatcgag tcggtcgagt tcttctggtt ttgagtcaat
     1621 ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat ccggcagctg
     1681 acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat
     1741 ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc
     1801 ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt
     1861 ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg
     1921 cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc
     1981 gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tggcaagatc
     2041 gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag
     2101 cgcgcccata tccagcgcca gtacccggcg gcctttcaac ccttccggca cttcgccgtt
     2161 gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat
     2221 cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc
     2281 atcacgacca atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt
     2341 atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc
     2401 acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat
     2461 gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa
     2521 gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcctg
     2581 tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt
     2641 gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga
     2701 acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa attggttgtc
     2761 gtgcccaagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg
     2821 atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt
     2881 catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc
     2941 ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt
     3001 cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac ggtcgccgcc
     3061 gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat aaatgtccgc
     3121 cagatactta tcaccatgct gaatgaaagc tgtactcgct tcggcgtcta ctgccataaa
     3181 cgcctcgcga acctccgccc ccacttcaaa cgcgcgtggg ccaattgccg gccctaacca
     3241 ggcgagaata ttttctggat tatcagcaaa acaggaaact gtctcttcca gcacgcctgc
     3301 gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa
     3361 cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc ctgccgggcg tattgctata
     3421 agaggcatct gctcgttttg aggcataagg ttcgccagta agattaagca catctttgcc
     3481 gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa aaagtcgctt
     3541 gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt tgagcgagtc
     3601 atacgggggc aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg
     3661 cagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct
     3721 tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc
     3781 cattccattt cgataccgga gatcgggtga taaagacgca gcatggttgc atgaagcgcc
     3841 tggcggtcaa atttacgcag cgtggagata aacgcttccg aagcaccttt tggcgggcgc
     3901 ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg
     3961 cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag
     4021 tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac cgccatgtga
     4081 gtacgtttgg tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga
     4141 ccaattgcca ccgcttcata ctcacgagta atttcacgcc tttgcaaaga ttcgactaaa
     4201 cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc tttatccaga
     4261 cgatggacga tgcctgcacg cggtacatca gcaatgggcg gatagtaatg aagtaacgca
     4321 ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccagatc gcgcggttta
     4381 ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca
     4441 aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc caatactttt
     4501 tctttcggct tatcacaaag tttgccgtta accagcacgc gctggtcgag gatccattct
     4561 tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga
     4621 ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct
     4681 tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct
     4741 ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa
     4801 cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg
     4861 ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc
     4921 acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga
     4981 taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctacgccta
     5041 ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta ttcgccttaa
     5101 cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct
     5161 ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg atcctcaaca
     5221 tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa acagtcagta
     5281 caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata
     5341 ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta accgcgtaga
     5401 aggcatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga
     5461 aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc
     5521 cgcaaacagc agcaatacat aacagaaacc tgaaacacaa aacggcagcc cttgagctgc
     5581 cgttttttta ttctgtcagt tgtgaaactg aagcgattta gtcactatcg ttctcatcaa
     5641 atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac
     5701 aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca
     5761 tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat
     5821 gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg
     5881 tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa
     5941 agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc
     6001 cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg
     6061 gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga
     6121 cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac
     6181 gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactaaagt
     6241 cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc
     6301 cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agaagaacaa taaggcctcc
     6361 caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaaccc
     6421 gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc
     6481 agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc atcgcccggt
     6541 acgtgatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca
     6601 ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag attccgtatt
     6661 aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact cagcacgcat
     6721 cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg
     6781 tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt
     6841 ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat
     6901 aaatgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg agatgacgtt
     6961 aactatcgac cattgtttgt tagtctccgg cacgactgat ttatccacca tcaatacggt
     7021 ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg
     7081 gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc
     7141 accgcatgtt gctgcgttag gaagcgaagc tggcggcact ttgtacggtt tgcaggtact
     7201 ggagcggatt gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcgcgtaa
     7261 agccattaac gtgtctgatc aggttccggc gaaaacgacg ttgttaatgg cgaccgggca
     7321 acaagccggt gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac
     7381 ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt atctggatat
     7441 tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac
     7501 ccgttcaatg aaggtattgg gctgttaccc tagtgagaac gtagtgcctg ttgatccaac
     7561 ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac tggcgattgt
     7621 cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga
     7681 accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca
     7741 actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg
     7801 acgacataat aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg
     7861 ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt
     7921 cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg
     7981 ccatattctg atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa
     8041 tttgctccag aaaccattgg tatgcttccg gtttacgtcc atcacaccag accacaactt
     8101 gctttgccag gctaccgctg tccggaccga acattgggtg taaccccagt accgggccat
     8161 cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga tcaaccagaa
     8221 tacaatcttt cggtaaaggc ggtaatttgc caataacttg ctcagtaacg tggattggca
     8281 cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt
     8341 gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac agacgtccca
     8401 tctgaccgcc gccgccaacg ataactaccg ggcgtaacgc aggacaaagc gttttaaatc
     8461 ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca
     8521 gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct
     8581 ctcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa
     8641 ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat
     8701 cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg agccgtcagc
     8761 tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccagctaatg
     8821 caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga
     8881 ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt gccatctttg
     8941 atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt attggaatta
     9001 ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca
     9061 cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag gatgacatgc
     9121 ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt aatgccaaca
     9181 aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg
     9241 gtgccgtttt taaagccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc
     9301 gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg
     9361 ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc
     9421 aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg
     9481 ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc
     9541 agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga
     9601 gcagtttccg gatcatgaat ggaacaaggg ccacatacta ccagcagacg aggatcgcgc
     9661 ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg
     9721 cttaatggaa aagcggcctt cagttgtgcc ggagtcatta aaacctgttc gtcggtaata
     9781 tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat
     9841 agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata
     9901 tttcgtacat ttttctttac ataagcaatt tattcgcgct ttcaaccctt gattctgtat
     9961 agataaattt acaccacctt tgaaaacaat ccgctatgct ttgaaaaagg agaaagaaat
    10021 gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgcc agattgatcc
    10081 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgttggta tggaagatgc
    10141 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
    10201 tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg attttactta
    10261 tgcccgagga ctttccggta aaagctttcc tgccagctgc aacaatgttg aaaatgccag
    10321 ccaactgcat gaagtatggc aaaaaggggc tgatgaaa
//
LOCUS       DAZFKY010000165        10235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFKY010000165 DAZFKY010000000
VERSION     DAZFKY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            157..1050
                     /locus_tag="ACHKCL_003762"
     CDS             157..1050
                     /locus_tag="ACHKCL_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764645.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            1057..1971
                     /locus_tag="ACHKCL_003763"
     CDS             1057..1971
                     /locus_tag="ACHKCL_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5764646.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2665..2883
                     /locus_tag="ACHKCL_003764"
     CDS             2665..2883
                     /locus_tag="ACHKCL_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5764647.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3100..3342
                     /locus_tag="ACHKCL_003765"
     CDS             3100..3342
                     /locus_tag="ACHKCL_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5764648.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3672..4601)
                     /gene="fdhE"
                     /locus_tag="ACHKCL_003766"
     CDS             complement(3672..4601)
                     /gene="fdhE"
                     /locus_tag="ACHKCL_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5764649.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4598..5233)
                     /gene="fdoI"
                     /locus_tag="ACHKCL_003767"
     CDS             complement(4598..5233)
                     /gene="fdoI"
                     /locus_tag="ACHKCL_003767"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5764650.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5230..6132)
                     /gene="fdoH"
                     /locus_tag="ACHKCL_003768"
     CDS             complement(5230..6132)
                     /gene="fdoH"
                     /locus_tag="ACHKCL_003768"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5764651.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6145..9195)
                     /gene="fdnG"
                     /locus_tag="ACHKCL_003769"
     CDS             complement(6145..9195)
                     /gene="fdnG"
                     /locus_tag="ACHKCL_003769"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8608..8610),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5764652.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQENYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9389..10222
                     /gene="fdhD"
                     /locus_tag="ACHKCL_003770"
     CDS             9389..10222
                     /gene="fdhD"
                     /locus_tag="ACHKCL_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5764653.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2604 a         2446 c         2597 g         2588 t
ORIGIN      
        1 atagttttct cataaatact ttaaaaatct atagctaaca aacattatgc aaagataatg
       61 ggctggcatc attgtcagta tcattcattt actgctctgg atgttataaa taattatgta
      121 gcatgtttgc tcttaaaccg tgggaaggag gctgtaatgg ttgccaatat taatctcatt
      181 aaacaagaaa gttattctgt tgttaattta gaaaaacaac taatgagtaa tgaatcaccg
      241 aagcaatcgc tattaaatga tttttcacat cgccttaaaa tggaaggtga ggaacaactg
      301 ttccattgtg ttaacgaact gaagtatggt ttgtcgagta ataatgaata tgacctgttt
      361 aaaaatagag aaacctcact gtggaaacgc aaagtaccag aaagcgcagc gctggcaatg
      421 acgggaacag tatataaatt atttggtagt cgcttactca acgcccgcaa tcagttagat
      481 aaaaatctcc tcgaaatggc tatgcaaatg gtttatggca aagatatggt gacaaagtcc
      541 ggggatatat tgattgatat acaattaaat aaggatggca gcctccagag taagcactac
      601 acatttgatg taggtgaagt tataaacagt tataatattg acctctttgt gaatgcttcg
      661 agtattaatc aaacttactt acatgataaa aatcctggtg atgttattag cctgtatggc
      721 actttagata acattcctct tgtagttaag gaatgcacgg gtaaaaatca tcaaattaac
      781 gttaaagcca aattgccaca agaacgaagt gaaaaagttg aggatatgtg tgaaagaatg
      841 agaggtggca tctctatgtt taatcacacc acaaaaacag ctggtaatat agagcataat
      901 ttgcagctaa gttttctcgt ggattcacgg ccgtcgatca ccaataaata ctcagccaaa
      961 gatgtagcgc cagacatact tgtattgccc gtcaacgtat atcatgcgga ttttaaaatt
     1021 aaaatcaata atgcagtaga gaagacttag tcaattgtgt tatgtgctgt gcaaaaaaat
     1081 ctccatgcag caaggaaggt tcttagcggg ataataggta atgtaccaaa aaataataaa
     1141 ccaaaaacaa tcatttttga tttacgtttt cttagtgcaa ttaagcagaa agtttttttg
     1201 ggcagtgaac aaccgttgtt tgtaaagcat accgcaatga tgagggaggc atgtaaggaa
     1261 ttaccgcagc tagtggagta tgttccttta gcctgtgaag cgcatagcca tcgcagcgtc
     1321 gcccttgcca aaaccgtggc tgcacccatc atggcgacag gtgtgggcgc ggctttaatg
     1381 tatgcaacat catcaggacc gtattatgcg caaagtcttt ctggttcgcc ggatatcgag
     1441 atgataaaaa aacgcatggc tgaattgttt aaccagtcgg atacttctgt gcgcaacaaa
     1501 tatcgcccgg cttttgataa atggaataaa ctggtagata aattaaatca cgaaagaaat
     1561 acagttccgt ttgcgtgttt gaccaccatt atagcgacag cgataaatga aactcctgag
     1621 ggggatacga atgttgccgt tgtgatgggg tgtaaatctg cgaaagatcg cacgatatcc
     1681 attgtccttg gcaactcgat gttgcaaaca ctctttgaaa aacgcctcgc cgatgatgga
     1741 gaaatagaaa acctttttga tcaacaggga tattttagtt gcgattcatt aacagccgaa
     1801 gaagtaatga tgttaaaaga cctttttgat attcgagttc ttcatctctc taacaaattt
     1861 aatgttgggt tgcaaggtaa tatcaacacg gatgttttac aagattcgtt ttttaaaaat
     1921 gtcgatttca ttcatgaatc taactcgtat accgtaggga tgggagtctg aagagtaaaa
     1981 ctcattagga ttatcagaat tcggccaatg cattgcaaaa atgaaataca aagagagatg
     2041 tgtgcaggcg cttagccgcg aggggcatgg cgaagccata cagaaagcac cggcattcag
     2101 aactcctgtg aaagcggcat agtgccgctg ctcaggagtt ttgttatgac ccgtatcgtg
     2161 ctgaacgttg ccaccctatg atgcgattaa aaaaactcac cgtcgagccg atgattgacg
     2221 tcaatgctat cggatgatgc cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt
     2281 taccaacaaa attataacga tgagcaagta aagaccggtt ggtggcagta ctgaccggtc
     2341 tgtgatggaa gattaatttc accttaaatc ctggtgctgt taggatgctg acgtttttcg
     2401 ggtacaccat tcagattatg caaataaaga ttcaatgaaa aaataaatat ccctgaatat
     2461 aatatattcg tagcacccat aactcatcct gggcgataac taaaccattt ttgagtaaca
     2521 aatgaaacaa ctgcaacgct cttattattt ctggaaaacg tctcgcaaaa acaaaacaac
     2581 atgcaaaaat ccgctttcgt taattaattt ttcggcgtaa ttttcgcaca tacattaatg
     2641 agtaatatat gtaaggtggt ggtaatggcg atgaatacgg tttttcttca tttatcagaa
     2701 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
     2761 ttacgcgaag cggtagagca atatctggaa cgtcagcaat tcccggtgcg taaagcaaag
     2821 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
     2881 tgacaaaaat ttttcattct tgaatataaa aaacagatgc ccttattctg gtattaatag
     2941 aaggctgttt tacttgaact tataataact gcaactgtta catcatatct ggaaaacgcc
     3001 tcgcaaaaat aaaaaatgat gcgtaaatga gctttcttta aagattcgac aagggtactt
     3061 ttctctccat agatatcaaa tgatattggt caaatattta tgaactctct ggcaggtatt
     3121 gatatgggca gaattatact cgatttatcg aatgaggtga ttaagcaact tgatgatctt
     3181 gaggtgcagc gtaatcttcc tcgtacggag ctattacggg aagcggtaga tcaatacttg
     3241 gtaaatcaat cgcaaacagt aagaaccagt gcttttggca tatggcaagg gtgtgaggaa
     3301 gatggtgtcg aataccagcg taagctgcgc gaggaatggt aatgggaaaa aatgatatca
     3361 atcagattgc tgacaacgtg cgcgttgttc atgccggatg cggcgtgaac gccttacccg
     3421 gcctacaaag tcgtataaat tcaatgtatt gcagtttatt ggtaggccta ataagcgcgg
     3481 cgcatcaggc aatcttacgc ttatcatcag tctcataccc ataaccttat tgatgtgggg
     3541 gtgggtaaat tgaggcaaaa caggcactgg cggcatagct tgaggtggta acgcggaata
     3601 cgagagatgg tgtcgtcaca ccctatgagc tgtaatccgg gcgaccagcg ccacccggaa
     3661 aacgtcagag attactcccc ttcacccgga aacaggaacg ggttgatgga actgcgggca
     3721 tagccttctt gctccattcg cgcgtccagt accagagagg cgaggtcatc tgccacggct
     3781 tcaactttcg gttctttttc ctgatagaga attttcaggt aagtgccgca gtcatcgcag
     3841 ctttcggctt taatcgcggc ctgttcgtca tccagcgacc agtaatgcag tttgccgctc
     3901 tgctcgcagt tgctgcattt tacgcgcaca acgtgccatt cagtttcaca caggttgcag
     3961 tgcaggtaac gtagcccctg agtggtgcca atttgcacca tgctggacac cggcatagaa
     4021 ccacaaaccg ggcaatattg acgttgctcg ccgtattcag cgcgggcttt gccggggatc
     4081 agattggcca tctgcgccca gtagagcgac agtgcagccc agataaacgg cgctttatcg
     4141 ctgctgacgg acgagaaatc agaggcaaac agtgcgctgg ccatatcttc cagctcctga
     4201 gtcgatgcct tctccagatt ctcaatcact gccagcgccg ggccgctcat ttcaggtttc
     4261 agctcagcaa tcagcgccat cagcagcttt tgccagtgct tatcacgcgg cagaacgtga
     4321 atatccagcg ggggcttgcc ttgtgcgctg gcttctttaa tgcgcgcagt cagatccatc
     4381 tccagcggat ggtcgtacag caccacttcc tgggcgtggg cgataagcgc agcaaagcgc
     4441 aggtaatcac ccagcggatt attttctgcc agctcgcgca gacgctcggc gcggcggttg
     4501 tataaattct tgagccgagg gaacaataac ggcggaatca tatccgccgt acgtttctcg
     4561 ctcgaaccca gctcatcttg cgggattatg cgaatactca ttcagctttc ttttccgttg
     4621 tcttgcggac ctcacggtac cagcgcgggt gatgtttctt cgcccaagcg ctggtaaccc
     4681 atccttccac catggcggta atcgtgcctt tcacccaaag ggcggcgtag atatgcacca
     4741 tgataaccac aattaacgcc actgcggcaa atgaatgcag cattaacgcg aatcggatca
     4801 ccgggattga gaaagcaggc gcaaaataag gacgccagat aatcacgccg ctcaccagca
     4861 gcagaaccag gaaaataatc gccgcccaga aaacgcattt ctgaccgaag ttataacgcc
     4921 cggtgtcacc tacttcctcg ttgacgacga tcttacgaat attcttcgcc caaaagatat
     4981 catcccgatt gattaggttg tgatgccagt aacggaaaaa catgatgatg aacgaggcaa
     5041 acataaccac gccgacaaac gggtgcagaa ttcgcgccag ctgcggtgtg cccatgattt
     5101 gcatcaacca gttgaaggac gggaacaaaa agcccagccc gctcaccgcc gccaggatga
     5161 agcagaaggc ggtgatccag tggttgatac gttccggcgc ggtgtagcgc acgatggtgt
     5221 cacgtcgttt catttgcgct cctcgtcttt ctcttcgtgc agattattct cttcctcatc
     5281 cgcatggttc ggaccgacac ccacgtagtg gaagatactg gctgcgaagg tagccgcaaa
     5341 gccaacagct gcgagcggtt tccagatgcc tttccagaat ttcacggttt cgctgatttc
     5401 cgggttctcc ggcaagccat gatacagatt tggcttgtca gcatggtgca gcacgtacat
     5461 aacgtgtgta ccaccgacgc ctgccggatc gtacaggccc gcattgtcgt aaccgcgggt
     5521 tttcagctcc gccacgcgct cgctcgccag cgttttcatc gactctttcg taccgaagtg
     5581 aatcgcgccc gttgggcagg tcttcacgca ggctggttct tgcccaacca ccacgcggtc
     5641 aacgcacagc gtacatttgt agacgcggtt gtcttccggg ttgaggcgcg gaatgtcgaa
     5701 cggacagccc gcaatgcaat aaccgcagcc aatgcactgc tcggactgga agtcgacgat
     5761 accgttggca tactgaataa ttgcaccttc cgccgggcac gctttcagac agcctggatc
     5821 ggaacagtgc atacagccgt ctttgcggat cagccattcc agtttgtcgt tctgctccac
     5881 ttccgagaag cgcatcaccg tccacgattt ggcgcttaaa tcattggggt tgtcgtacac
     5941 cccaatgtta ttgccgacgg tatcgcgaat gtcgttccac tctgaacacg ccacctgaca
     6001 ggctttacag ccgatacagg tggtgacgtc gatgagtttc gccacttctt cctggaagtc
     6061 ccgcgcctga ggcgcggggg tcagaccgtt agtcgcggaa cgacgaatga tatcttgaga
     6121 ttgataagcc atatgtcgtc tccgttacac cttttccaca ttcacaagga aggacttaaa
     6181 ctccggcgtc tgcgtgttcg catcaccgac gaatggcgtc aacgtattgg caataaagcc
     6241 ttttttcgca acaccttcat agccccagtg aataggaata ccgatggtat cgatatcttt
     6301 gccgttcgct ttcagcgtgc gaatacgttt ggtcaccacc gctttggctt tgatatagcc
     6361 acggttggag gagactttca cggtatcgcc ctgggcaatg ccaagtttat tcgccagcga
     6421 ctccccgatt tccacaaact gctctggttg caaaatcgcg ttcaacagcg cgtgtttggt
     6481 ccagtagtgg aagtgctcgg tcagacgata ggtggttccg acatacggga acttatcggc
     6541 tttacccaat gcttcggcgt cgtctttaaa gatacgtgca gccgggttcg agataacgtt
     6601 tggatgcagc gggttagttc ccagcggcgt ttcaaacggc tcgtagtgtt ccgggaacgg
     6661 accttccgcc atcttatcga gggcaaacag acgccccatg ccttcctgct gcatgataaa
     6721 cggcccgacg ccgctacccg gaggcgctgc gctgtagtcc ggaatatccc agccggtcca
     6781 cttagtgccg tcccatttca gcaactgacg cttcggatcc cacgggttac cctgcggatc
     6841 tgcggaggcg cggttataca gaatgcggcg gttaagcggc catgcccatg cccaacccag
     6901 cgtgttaccg aggccagacg gatcggcgtt atcacggcgt gccatctggt tgccttccgg
     6961 cgtccagcta ccggcgaaaa tccagcaacc acaggaagtt gtaccgtcat cgcgcagttg
     7021 ggcaaacgag ctaagttgtt ggcctttctt gacgataacc gcaccggttg ccggatcggt
     7081 aatatcggcc agcgccttac cgttgctctc catcgctact tcttccgact tcggctcatg
     7141 cggaatggcg tagttccagg tcatgttcag cacctggtcc gggttcgcgc caccctgttc
     7201 ggcatacatc ttgcgcaagc gcaggaagat accggagagg atctcgccat cagtcagcgc
     7261 aatccccggg gcgtccgcac ctttccagtg ccactgcaac cagcgacctg agttaacgat
     7321 tgaaccgttc tcttctgcga agcaggtcga tggcagacgg aacacttcgg tctggatctt
     7381 cgacgagtca acttcgttca gctcaccgtg gttctgccag aagttagagg tttcagtgtt
     7441 cagcgggtcg atggtgacga ggaacttcag tttcgacaga caactgatca ctttgttttt
     7501 gttcgggaat gaggcaacag ggttaaagcc ctggcagata tagccattga ccttgccttc
     7561 tttcatcatc tcgaagtatt gcaggacgtc gtagccttta tcccacttcg gcaaccagtc
     7621 aaagccccag ctattttctg ccgtcgcttt atcaccaaag aaggccttca tcatagagac
     7681 gaagaatttc gggtagttgc cccagtagtt ttcctggcct tccagcagcg gttttggcgt
     7741 gttggcggta aggtaggttt gcagatcggt ctgcttctcg cttggcagcg tcatgtaacc
     7801 tggcaggctc tgcgacagca gccccaggtc cgtcagcccc tgaatattgg agtgaccgcg
     7861 cagggcgtta acgccgccgc cagccatccc catgttaccg agcagcagct ggatcatcgc
     7921 catcgtacga atgttttgcg caccaacgga gtgttgcgtc cagccgaggg catacaggaa
     7981 cgaggcagtt ttatcgtgag cactggtttc tgcgatgtat tcgcagactt tcaggaacgc
     8041 gtcttttggc gtaccacaga tgttttcaac cacatctggc gtgtagcggg aaacgtgctg
     8101 tttcagcaag ttccacacgc agcgcggatg ttgcagcgtg gtatcgcgtt tggcgaagcc
     8161 gttttcgtcc agttcataag tccaggagga tttatcgtac ttgcgttttt ccgcgtcgta
     8221 gccggtgaac aggccatctt caaagccgta atcctcacgc acgatcaggc tggcgttggt
     8281 ataggcttca gtgtattcgc ggttgaattt ttcattgttc agcaggtaca gcaatacgcc
     8341 tgacaggaaa gcaatgtcag taccggaacg aataggggca tagtagtcag ccaccgcagc
     8401 cgtacgcgta aagcgaggat cgatcacaat cagcttcgcg ccattgtgaa ttttggcttc
     8461 catcgcccag cggaacccga ccgggtgagc ttcagcggcg ttaccgccca tcaccacgac
     8521 gaggttggcg ttcttgatgt cgacccagtg gttggtcatc gcaccgcgac caaatgttgg
     8581 agcaagactt gctaccgttg gtccgtgtca gacacgcgcc tggttgtcga ccgcgagcat
     8641 acccagcgcg cgggagaatt tttgcgttaa atagccggtt tcgttactcg acgcggaagc
     8701 acacagcatc ccggtggaga gccagcggtt aacagtcacg ccttcggcgt tttgcgcaat
     8761 gtagttagca tcgcggtctt ctttcatcag tttggcgatg cgatcaaacg cctcttccca
     8821 actgatttgc tgccatttat cagaacctgg cgcacggtat tccggaaact tcagacggct
     8881 ttcggagtgg atgaaatcca ccaggccagc gcctttcgga caaagtgcac cgcggttgac
     8941 cgggtgatcc ggatcgcctt cgatatggaa gatagatgct ttggcgtttt ttgctccgtc
     9001 accgaggctg tacatcaaca gcccacagcc tacggaacaa taggtgcagg tattacgggt
     9061 ttcgcgggtg cgcagcagtt tatactgccg ggtttccgcg agtgctacgc tgggtgcaaa
     9121 acccagtgcc gctgccgtgg tgcctgccat accgccagcg cagatcttaa agaactgcct
     9181 tctgctgacc tgcatggatt gctccttgtt tcgacattgt cacgtcccat ttacattcgc
     9241 ttgctgcgtg tgcagggagt gggagttatt tttctttgcg gaaggggccg caaaggtcca
     9301 gaattggctc aatttccctc catccaggaa gggtttgtaa cagaatacca taatgttggt
     9361 gtgtgtgttc ttatctggtt aagagaaagt gaaaaaaaca cagcaaaaag aaatcgaaaa
     9421 tgtgacaaat atcacaggtg ttcgtcaaat tgagttatgg cggcgtgatg atttacaaca
     9481 cccacggtta gatgaggtcg cggaagaagt tcccgttgcg ctggtctaca acggcatttc
     9541 gcatgtggtg atgatggcgt cgcccaaaga ccttgagtac tttgcgctcg gtttttcgct
     9601 ttccgagggg ataatagaaa gtccgcgcga tatcttcggc atggatgtcg ttccttcctg
     9661 taatggtctt gaagtacaaa ttgagctttc cagccgccgc tttatggggt tgaaggagcg
     9721 ccgccgggcg ctggcgggac gtacgggctg tggcgtatgc ggcgtggagc aacttaatga
     9781 catcggaaaa ccggtgcagc cgctaccgtt cacccagacg tttgatctca acaaactgga
     9841 tgatgcatta cgtcatctca acgattttca gccagtggga caactgaccg gttgtactca
     9901 cgccgccgcc tggatgttgc catctggcga actggtcggc gggcatgaag acgtgggtcg
     9961 ccatgtggcg ctggacaaac tgttaggccg ccggtcacaa gaaggggaaa gctggcagca
    10021 aggtgcggta ctggtttcca gtcgtgccag ttatgaaatg gtgcaaaagt cggcgatgtg
    10081 cggcgtagag attttgtttg cggtgtctgc cgcgaccacg cttgctgtag aagtggctga
    10141 gcgctgtaat ctgacactgg taggtttttg taaaccgggt cgggcaacgg tttataccca
    10201 tccgcagcgt ttgagcaatt aattaaaata agtcc
//
LOCUS       DAZFKY010000166        10214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFKY010000166 DAZFKY010000000
VERSION     DAZFKY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            257..4510
                     /gene="fdeC"
                     /locus_tag="ACHKCL_003771"
     CDS             257..4510
                     /gene="fdeC"
                     /locus_tag="ACHKCL_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5764654.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDTNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(4631..5488)
                     /locus_tag="ACHKCL_003772"
     CDS             complement(4631..5488)
                     /locus_tag="ACHKCL_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5764655.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDTYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5737..6606
                     /locus_tag="ACHKCL_003773"
     CDS             5737..6606
                     /locus_tag="ACHKCL_003773"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5764656.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6766..7359)
                     /gene="rclC"
                     /locus_tag="ACHKCL_003774"
     CDS             complement(6766..7359)
                     /gene="rclC"
                     /locus_tag="ACHKCL_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5764657.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(7371..7607)
                     /gene="rclB"
                     /locus_tag="ACHKCL_003775"
     CDS             complement(7371..7607)
                     /gene="rclB"
                     /locus_tag="ACHKCL_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5764658.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(7716..9041)
                     /gene="rclA"
                     /locus_tag="ACHKCL_003776"
     CDS             complement(7716..9041)
                     /gene="rclA"
                     /locus_tag="ACHKCL_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5764659.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            9267..10121
                     /gene="rclR"
                     /locus_tag="ACHKCL_003777"
     CDS             9267..10121
                     /gene="rclR"
                     /locus_tag="ACHKCL_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5764660.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2760 a         2292 c         2532 g         2630 t
ORIGIN      
        1 taacttaagt atctatttaa tgacttgcac aaaaagctaa attttcaccc ataaataaaa
       61 atataatccc gcgcccaacc acctgatgag tggctacagg cactggatat attaggtggc
      121 ggtgtacttt cttacataaa ggtatttcct tttctgcgga aaaggaaacc gggaaatccc
      181 cggtttttct gacaagcaga cgccattatt tgtgtctgcc tatgttcgtt aattcgttca
      241 tcaggaaatt atctcaatgt cacgttataa aacaggtcat aaacaaccac gatttcgtta
      301 ttcagttctg gcccgctgcg tggcgtgggc aaatatctct gttcaggttc tttttccact
      361 cgctgtcacc tttaccccag taatggcggc acgtgcgcag catgcggttc agccacggtt
      421 gagcatggga aatactacgg taactgctga taataacgtg gagaaaaatg tcgcgtcgtt
      481 tgccgcaaat gccgggacat ttttaagcag tcagccagat agcgatgcga cacgtaattt
      541 tattaccgga atggccaccg ctaaagctaa ccaggaaata caggagtggc tcgggaaata
      601 tggtacagcg cgcgtcaaac tgaatgtcga taaagatttc tcgctgaagg attcttcgct
      661 ggaaatgctt tatccgattt atgatacgcc gacaaatatg ttgttcactc agggggcaat
      721 acatcgtaca gacgatcgta ctcagtcaaa tattggtttt ggctggcgtc atttttcagg
      781 aaatgactgg atggcggggg tgaacacctt tatcgaccat gatttatccc gtagtcatac
      841 ccgcattggt gttggtgcgg aatactggcg cgattatctg aaactgagcg ccaatggtta
      901 tattcgggct tctggctgga aaaaatcgcc ggatattgag gattatcagg aacgcccggc
      961 gaatggttgg gatatccgcg cagagggcta tttacctgcc tggccgcagc ttggcgcaag
     1021 cctgatgtat gaacagtatt atggcgatga agtcgggctg tttggtaaag ataagcgcca
     1081 gaaagacccg catgctattt ctgccgaggt gacctatacg ccagtgcctc ttctgacact
     1141 gagcgccggg cataagcagg gcaagagcgg tgagaatgac actcgctttg gcctggaagt
     1201 taactaccga attggcgaac ctttggcgaa acaactcgat acggatagca ttcgcgagcg
     1261 tcgggtactg gcaggcagcc gctatgacct ggttgagcgt aataacaaca tcgttcttga
     1321 gtaccgcaaa tctgaagtga tccgtattgc tctgcctgag cgtattgaag gtaagggcgg
     1381 tcagacactt tccctggggc ttgtggtcag caaagcaact cacggactga aaaatgtgca
     1441 gtgggaagcg ccgtcattac tggctgaagg tggcaaaatt accggtcagg gtagtcagtg
     1501 gcaagtaacg ctcccggctt atcgtccagg caaagacaat tattatgcga tttcagcagt
     1561 tgcctacgat aacaaaggca atacctcaaa acgcgtgcag acagaggtgg tcattaccgg
     1621 agctggtatg agcgccgatc gcacggcgtt aacgcttgac ggtcagagcc gtattcaaat
     1681 gcttgctaac ggtaatgagc aaaaaccgct ggtgctgtct ctgcgcgacg ccgagggcca
     1741 gccagtcacg ggcatgaaag atcagatcaa gactgaacta actttcaaac cggctggaaa
     1801 tattgtgact cgttccctga aggccactaa atcacaggca aagccaacac tgggtgagtt
     1861 caccgaaact gaagcagggg tgtatcagtc tgtctttact accggaacgc agtcaggtga
     1921 ggcaacgatt actgttagcg ttgatggcat gagcaaaacc gtcactgcag aactgcgggc
     1981 cacgatgatg gatgtggcaa actccaccct gagcgctaac gagccgtcag gtgacgtggt
     2041 tgctgatggt cagcaagcct atacgttgac gttgactgcg gtggactccg agggtaatcc
     2101 ggtgacggga gaagccagcc gcttgcgatt tgttccgcaa gacactaatg gtgtaaccgt
     2161 tggtgccatt tcggaaataa aaccaggcgt ttacagcgcc gcggtttctt cgacccgtgc
     2221 cggaaacgtt gttgtgcgtg ctttcagcga gcagtatcag ctgggcacat tacaacaaac
     2281 gctgaagttt gttgccgggc cgcttgatgc agcacattcg tccatcaccc tgaatcctga
     2341 taaaccggtg gttgggggga cagttacggc aatctggacg gtaaaagatg cctatgacaa
     2401 ccctgtgacc agcctcacgc cggaagcgcc gtcattagcg ggtgccgctg ctgaaggttc
     2461 tacggcatcg ggctggacaa ataatggtga tgggacgtgg actgcgcaga ttactctcgg
     2521 ctctacggcg ggtgaattag aagttatgcc gaagctaaat ggacagaatg cggcagcaaa
     2581 tgcggcaaaa gtaaccgtgg tggctgatgc gttatcttca aaccagtcga aagtctctgt
     2641 cgcagaagat cacgtaaaag ccggcgaaag cacaaccgtg acgctggtgg cgaaagatgc
     2701 gcatggcaac gctatcagtg gtcttgcgtt gtcggcaagt ttgacgggga ccgcctctga
     2761 aggggcgacc gtttccagtt ggaccgaaaa aggtaacggt tcctatgttg ctacgttgac
     2821 tacaggtgga aagacgggcg agcttcgcgt catgcctctc ttcaacggcc agccagcagc
     2881 caccgaagcc gcgcagttga cggtcatcgc cggagagatg tcatcagcga actctacgct
     2941 tgttgcggac aataaggctc cgaccgtcaa aacgacgacg gaactcacct tcaccgtgaa
     3001 ggatgcgtac gggaacccgg tcaccgggct gaagccagat gcaccagtgt ttagcggtgc
     3061 cgccagcacg gggagtgagc gtccttcagc aggaaactgg acagagaaag gtaatggggt
     3121 ctacgtgtcg accttaacgc tgggatctgc cgcgggtcag ttgtctgtga tgccgcgagt
     3181 gaacggccaa aatgccgttg ctcagccact ggtgctgaac gttgcaggtg acgcatctaa
     3241 ggctgagatt cgtgatatga cagtgaaggt taataaccaa ctggctaatg gacagtctgc
     3301 taaccagata accctgaccg ttgtggacac ctatggtaac ccgttgcagg ggcaggaagt
     3361 tacgctgact ttaccgcagg gtgtgaccag caagacgggg aatacagtaa caactaatgc
     3421 ggcaggtaaa gcggacattg agcttatgtc aacggttgcg ggagaacaca atatttccgc
     3481 ttcggtgaat ggtgctcaga agacggtcac ggtgaaattc aacgcggatg ccagcaccgg
     3541 tcaggcaaac ctgcaggtag acgccgctgc tcaaaaagtg gcaaacggca aagatgcctt
     3601 tacgctgacg gcgaacgttg aggataaaaa tggtaaccct gttccaggga gcctggtgac
     3661 ctttaatctg ccccggggtg tcaagccgct tacaggcgat aatgtctggg tgaaagccaa
     3721 cgatgagggg aaagcagagt tgcaggtggt ttcagtgact gccggaacgt atgagatcac
     3781 ggcatcggca gggaatagcc agccttcgaa tacgcagact ataacgtttg tagccgataa
     3841 ggctaccgca accgtctccg gtattgaggt gattggcaac tatgcactgg cggacggcaa
     3901 tgccaaacag acgtataaag ttacggtgac tgatgccaat aacaacctgt tgaaagatag
     3961 cgaagtgacg ctgactgcca gcccggcaaa tttagttctg actcccaatg ggacggcgaa
     4021 aactaatgag caaggacagg ctattttcac cgccacgacc actgtcgcag cgaaatatac
     4081 actcacggcg aaagtgagtc aggccgacgg tcaggaatcg acgaaaactg ccgaatctaa
     4141 attcgtcgcg gatgatacaa atgcagtact caccgcatca tctgatgtga cttctctggt
     4201 ggcggatggg atatcgactg cgaagctgga ggtgacactg atgtcggcaa ataaccccgt
     4261 tggggggaat atgtgggtcg acattaagac gccagaaggg gtgacggaga aggattatca
     4321 gttcctgccg tcgaaaaatg accatttcgt gagcggaaaa atcacgcgta catttagtac
     4381 cagcaagcct ggtgtctata cgttcacatt taacgccctg acgtatggcg ggtacgaaat
     4441 gaagccagtg acggtgacca ttaccgcggt ggatgccgat acggcaaagg gcgaggaggc
     4501 gatgaactaa tattaagaat atggaagtaa cattctaata aacaataatg gcgttgacat
     4561 cttcaacgcc attatttatt gaattgcctg acagtagtct cgttataagt tcatttagat
     4621 atagcgacag ctaaaccgga cgaacaggga taaatatttc gcaaacaaca tcattgctat
     4681 ccttttctac gaaatcttca ttgtaataaa accgctcaat atcactgccg ttcactctgg
     4741 ctaaccccct ggcgggtaat tccatcagat agaggtttcg cgcccattca gggtagtgct
     4801 cccactcacc ttcatagcgg aaagaggcat acagtcctcg gttaattgtc cactctttcg
     4861 gcatctctga atcggtttgt ttgcataccg taccggcaaa agtttcaact gtgagtaaat
     4921 cgaccgattt tgttgacggt tcaaacgtgg agacgaaata ggtatccgtc ttaaaattct
     4981 tcatacatct ggctaaatga tgacgtcgca gtttgacttc ttcatccggt gatttattcg
     5041 acacttcaat atcatattta aatgaattgc caaaaatagg tgtctcagga aagttgacta
     5101 atctacactc cgtcttttgc tgttgacgta ttaaaaaaga agggaagata tttgcgagat
     5161 cccaataatc acggtggcgg tatacacggg gagagcagcc gaataacttt ttaaattcgc
     5221 ggctgaatga ctgctgtgaa tcaaaatgca aagagattgc aatatcaagc atagatttcg
     5281 cggtgagccg gacaagaatg gcggcacgac aaagccttcg tttgcgaatg tattctccca
     5341 aaggcacatg catgaaattt cggaacagaa gctggatgtt gcgtctgctg tagccagatt
     5401 tctgtgcgat atcttcgatt gaaatagggt gctcaagatt gcactcaatc cactccagga
     5461 gctgctgtag aatcttctgc ctgatcatca cattctccgt taattctttg tcatatcgca
     5521 cattttcgtt atgccctggg ccctaagtgt atcaaaaaca gcaagggcga aggcaaaagc
     5581 actggtagcg tctgcttcgt tgtccccgaa aatagagagg tttcagtcct acattattaa
     5641 tgaatttttt gcataagtga tatcaaaatc cacatactaa tttgaggtta cgttttaacg
     5701 tagactcatt gttcatgcct aatggaggga ctgacagtgg aattttccgt attaagtaac
     5761 aatctgaaaa tgccgatgat gggatttggt gtttttcagg ttaccgataa aaacgtgtgc
     5821 aaacagtcag tgttgaacgc tatccgcacg gggtatcgac tcatcgacac tgcagcggta
     5881 tacggtaatg aagatgccgt cggtgaagcc gttcgtgaag ctatatccga aggtttatgt
     5941 acccgagaag agttatttat aacgtctaaa ttgtgggtgc aggatatgtt gaatcaggat
     6001 acggcagcag caggtattga agcatcatta aaaaaatcgg gactagaata cttcgacctc
     6061 tatttattgc atcaggctat gcgcgattat ttcagtgcgt ggcgtgcact cgaagatgcc
     6121 tatgaagagg gcaaattaaa agcgattggg gtttccaatt tctatcctca tgttctggcg
     6181 aacttttgtg aaacggtaag agttaaaccg atggtcaacc aggtcgagtt gcatccctat
     6241 tttgcccaac cagaggcgct ggcaaccatg aagtattata acgtgcagcc tgaagcatgg
     6301 gctccgttag gtggtggacg acataaaccc tttgaaaata atctgcttca gagtattgca
     6361 gatgcccatc aaaaatcgat ttctcaagtc attctgcgtt ggaatattca acggggagtg
     6421 gtcgttattc cgaaatcgac acatcaacag cgtatcgaag aaaattttgc tatctgggat
     6481 ttctcactga cagagaaaga aatggcacaa attagttcgc ttgatttggg ttatgttggg
     6541 gagtcggtaa aacattttaa tcctgaattt gttcgtggtt gtcttgctgt aaaaatacat
     6601 gattgatatt aatcatgtat tttacctgag acgacaagaa tcttttaaca ggggagtgat
     6661 attgatcttc actctgtcat atctccggta atatggcgtc aggcttcatc atcagaaagg
     6721 gggcctgacg cctgaaaaag tgaacaacag acagtgttcg gattatcaat attcagtttt
     6781 taacgttgaa ctggattcat tactgccttg tttaagaatt tcccgcgccg aatctgccat
     6841 tatcatgaca gcacctgcca gcatcagagt atctttcaat accagacgac cagcaccgga
     6901 taaataaggg aaaccatgat gagcgtcacc caatgcgggt acccatgcct ccggggtggt
     6961 gattaaaaat gagagtgtta ccagcggtgt ggtaaatgcc atcagccctc ccaataaacc
     7021 taaccagcga ttgacaggat tagccaaaac cagcagagca ataatcacct ccacgacgcc
     7081 aagaccgttg gaaaaaccat aggtattatt ggccgattgc catgcccttg cttctggttt
     7141 gtattcgcct tcgtgagtca gatactgttt atagtcttcc gggtgttcat aaaagaacga
     7201 cattagtgga ctgtttgcga cgaatggtgt aatgctgtct gcctcgtaag ggacaaactt
     7261 taataaccca atccacataa aaacaatcgc aatactcaga cgaatcaatg ccaggccaat
     7321 tttatctccc cgacttaaca ggtgcaggta tttttccatt gtgaatatcc ttatttatac
     7381 aatactgctg ttgcggttga attattatca attcgcatcg atgtgacttt ccaggatgaa
     7441 gcattatatt ttctggctat atcagcaatg gcatcctcca gaactgtcgg tgatgctgat
     7501 actgacgaga cgctgatatg tttcagaatt attttatcat ctgcattggt ggaaaatgcc
     7561 ataacgccag ataatagtgt tgcaaataaa acggattttt taaacatata aaataccatc
     7621 aatgaagtga ttaagaagtt atagagtaac agggaattaa taagattctt ttctctgaga
     7681 cgccagaata tttgttctgg cgtctgattt tgagtttatt tgactaatga aaatagatca
     7741 ttgagtgatt cgctcatcga cggatgagta aatatctgat cgcgtaatat gctataaggc
     7801 agcccggcat ccatcaccat tttcactata ttaatcatct cgtgggagtc aacacacagc
     7861 agtgatgccc ctaatatacg ttgggtttta ttatcaacaa tcgcttttaa taccccacgg
     7921 gtatcgttca tcactctggc gcgcggaatt gcagctacag gcaatgtcac cacctgaata
     7981 tcagcaccac tctctctggc ttgttcttct gtcataccaa ccctggacag gggcggtgtc
     8041 ataaatacgg aataaggcac atttttccga tcatcagtac tacgtttgcc ttcacccagt
     8101 aactcatcac gtacaatgcg gtaatcatcc agtgatatgt aagtaaattg cagcccgccg
     8161 gtaacatctc ccatcgccca aatattgtct gcggtggtat gtaatcgctt gtcaacgaca
     8221 attgccccgc gctcgtttac tgcgataccg gcattttctg gatgtaacga agcggtagcc
     8281 ggttgacgac cggaagctat taacagtgca tccaccgcca gttgggcgtg ctcgctatgc
     8341 acttgcactt gattttcatg gtgactgatt cgctccacat gggcattgag gataatatcg
     8401 acgccctgat cgcgtaaaat cgtcgcgata ttatcagcaa tatcccgttc ttcccgaggc
     8461 aaaaacagcg aagctgcttc taaaatggtt accttgctgc caaaattagc gaacatagag
     8521 gcgaactcaa cgccaatata tccgccgccc aaaataccta aatgcccagg caattctttt
     8581 agattaagta atccggtgct gtcatatact cctggcgtgg tggtaattcc aggaattggc
     8641 ggaaccacgg tttgtgcacc ggtattaata aaaattttct cgccatgaat ttccagattt
     8701 ccctcaggcc gatgaacacg caggctatga ttattgataa actccgcctg gccgttgatc
     8761 acgtcgatat tgggcatatc tgcaagatta tgaaaattct tattacgtaa aaaattaacc
     8821 acttcatttt tacgctgtat ggctcggaca aaatctgtgt gctgctgtgc gtcatgaacc
     8881 aatgttttgg ttgggatgca gccgatatta atacaggtcc cgccatacat tgcatttgat
     8941 tgttcgatga gagccactcg ccaacctgct tttgccagcg tgacggctaa tgtttttcca
     9001 gccttgccaa aaccaataat cactgcctga tatttattca tgatgatttc ctgtcgaaac
     9061 gcgttcattt gacatcatga ctatagacgc aaaagagcaa ataacatttc ttcacaacgc
     9121 ttgatatttt gtcttatcgt ctggtatttt ctttttctta atcagaatca caaaacaaaa
     9181 atcatgatta actatttgat ggtattagat tagttgttta ctaagattgt tggtgtttgt
     9241 aatcaaaaac cactcaggag tctgatatgg atgcccttag ccgtttgttg atgcttaacg
     9301 ctccacaagg aacgatcgat aagaattgcg tgttaggaag tgactggcag cttccacatg
     9361 gtgccgggga attatcagtt attcgttggc atgcgttaac gcaaggagcg gcgaagctgg
     9421 aaatgccgac gggagagatt tttacattac gcccgggaaa tgtggtcctg ctaccacaaa
     9481 attcagctca tcgcctgagt catgtcgata atgaatcgac ctgtattgtc tgtggtactc
     9541 ttcggctgca acattcggca cgttattttt taacgtcttt gccggaaacg ctgtttttag
     9601 caccggttaa ccacagcgtg gaatataact ggctgaggga ggcgatcccg tttttacaac
     9661 aggaatccag atcggcaatg ccgggagtgg atgcactgtg tagccagatc tgcgctacat
     9721 tctttaccct cgcggtgcgt gagtggattg cacaggttaa tactgagaaa aacattctca
     9781 gtttgcttct gcatccacgt cttggtgcgg taatacagca aatgctggaa atgccaggac
     9841 acgcctggag cgtcgaatcg ctggccagca tcgcccacat gtcccgggca agttttgccc
     9901 agcttttccg tgatgtttcc ggaaccacgc cgctggctgt attaacaaag ttgcgtctac
     9961 aaatagcggc ccagatgttt tcccgggaaa tgctccctgt tgtggtgatc gctgagtcag
    10021 taggctatgc cagtgaatca tcttttcaca aggcgtttgt ccgcgagttt ggttgtactc
    10081 cgggagaata tcgggaaagg gtcagacagc ttgcaccctg aataaaaccg ccagaaatca
    10141 gggcaaagat aatccgcatt ccgggagttg tgagcctttc aactatttct atttccaacg
    10201 gtggtttggg cttt
//
LOCUS       DAZFKY010000167        10187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFKY010000167 DAZFKY010000000
VERSION     DAZFKY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            88..663
                     /gene="ssuE"
                     /locus_tag="ACHKCL_003778"
     CDS             88..663
                     /gene="ssuE"
                     /locus_tag="ACHKCL_003778"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5764661.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            656..1615
                     /gene="ssuA"
                     /locus_tag="ACHKCL_003779"
     CDS             656..1615
                     /gene="ssuA"
                     /locus_tag="ACHKCL_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5764662.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1612..2757
                     /gene="ssuD"
                     /locus_tag="ACHKCL_003780"
     CDS             1612..2757
                     /gene="ssuD"
                     /locus_tag="ACHKCL_003780"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5764663.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2769..3560
                     /gene="ssuC"
                     /locus_tag="ACHKCL_003781"
     CDS             2769..3560
                     /gene="ssuC"
                     /locus_tag="ACHKCL_003781"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5764664.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIVFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3557..4324
                     /gene="ssuB"
                     /locus_tag="ACHKCL_003782"
     CDS             3557..4324
                     /gene="ssuB"
                     /locus_tag="ACHKCL_003782"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5764665.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4367..6979)
                     /gene="pepN"
                     /locus_tag="ACHKCL_003783"
     CDS             complement(4367..6979)
                     /gene="pepN"
                     /locus_tag="ACHKCL_003783"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5764666.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7245..8447
                     /gene="pncB"
                     /locus_tag="ACHKCL_003784"
     CDS             7245..8447
                     /gene="pncB"
                     /locus_tag="ACHKCL_003784"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5764667.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8616..10016
                     /gene="asnS"
                     /locus_tag="ACHKCL_003785"
     CDS             8616..10016
                     /gene="asnS"
                     /locus_tag="ACHKCL_003785"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5764668.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2275 a         2622 c         2769 g         2521 t
ORIGIN      
        1 atttggcatt ttcttgtctc tccgacagac tgagcgaatt gttatcaatg ttaacaaaac
       61 aagaacaatt ggttataagg agagagtatg cgtgtcatca ccctggcggg tagtcctcgc
      121 tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa tggcctggat
      181 gttgaggttt atcactggaa tctgcaaaac ttcgccgcgg aagatctgct ttatgctcgt
      241 ttcgatagtc cggcactcca taccttcacc gaacagttgc aacaggctga cgggctgatt
      301 gtcgccacgc ctgtgtataa agccgcttat tccggtgcgc tgaaaaccct gctcgacctg
      361 ctgccagaac gtgcgttgca aggcaaagtg gtgctaccgc tggcgacggg cggtacggtg
      421 gcccatctgc tggcggtcga ttatgccctt aagccagttt taagtgcgct gaaagcccag
      481 gagatcctgc acggcgtgtt tgccgatgac tcacaagtaa ttgattacca tcacaaaccc
      541 cagttcacgc cgaatttaca aacccgtctt gataccgcgc tagaaacttt ctggcaggca
      601 ttgcaccgcc gcgatgttca ggttcctgat cttcattctc tgcgaggtaa tgcccatgcg
      661 taacatcatt aaactggcgc tggtgggatt gcttagcgtc tctacgattg tggttgctgc
      721 agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg gtatggtatt
      781 ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa tcaaaaatct cgtgggtgga
      841 gttccccgct ggcccgcaaa tgcttgaggc gttaaacgtt ggcagtattg atctcggcag
      901 taccggggat attccgccaa tcttcgccca ggctgccggg gctgatttgc tgtacgtggg
      961 cgtcgagccg ccgaagccaa aagccgaggt gattctggtg gcagaaaaca gcccgatcaa
     1021 aaccgcagcc gatcttaaag gccacaaagt tgcgttccag aaaggttcca gttcacacaa
     1081 tcttttactg cgcgcactac gccaggccgg acttaagttt actgacatcc agcccactta
     1141 tctgacgcca gctgatgccc gcgccgcgtt ccagcaaggt aacgttgacg cctgggctat
     1201 ctgggatccc tactactccg ctgcattatt acagggcggc gtgcgggtgt tgaaagacgg
     1261 caccgatctc aatcaaaccg gatcgtttta tctggcagct cgcccctatg cagaaaaaaa
     1321 cggcgctttt attcagggtg tactggcaac ctttagtgag gccgatgcgt taacccgcag
     1381 ccagcgcgag caaagtatcg ctttactggc aaaaacgatg ggcttaccgg caccggtgat
     1441 tgcctcttac ttagatcatc gccctcctac caccatcaaa ccggttaacg ccgaggttgc
     1501 cgccttacag cagcaaacgg cagatctgtt ttatgaaaat cgtctggtgc cgaaaaaagt
     1561 cgatattcgc caacgcatct ggcagcccac tcaactggaa ggaaaacaat tatgagtctg
     1621 aatatgttct ggtttttacc gacccatggt gacgggcatt atctgggaac ggaagaaggt
     1681 tcacgcccgg ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga tcgtcttggc
     1741 tataccggtg tgctgatccc aacggggcgc tcctgcgaag atgcgtggct ggttgccgca
     1801 tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgtcc cagcgtaacc
     1861 tcacctaccg ttgccgcccg ccaggccgcc acgcttgacc gtctctcaaa tggacgtgcg
     1921 ttgtttaacc tggtcacagg cagcgatcca caagaactgg caggcgacgg cgtgttcctt
     1981 gatcatagtg agcgctacga agcctcggcg gaatttaccc aggtctggcg gcgtttattg
     2041 ctgggggaaa ccgtcgattt caacggtaaa catattcatg tgcggggagc caaactgctc
     2101 ttcccgccga ttcaacagcc gtatcctccg ctttactttg gcggatcgtc agacgtcgcc
     2161 caggagctgg cggcagaaca ggttgatctc tacctcacct ggggcgaacc gccggaactg
     2221 gttaaagaga aaatcgaaca agtgcgggcg aaagctgccg cgcatggacg caaaattcgt
     2281 ttcggcattc gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg gcaggccgcc
     2341 gagcggttaa tctcgcatct tgatgatgag actatcgcca aagcacaagc cgcattcgcc
     2401 cgcacggatt ccgtggggca acagcgaatg gcagcgctac ataacggcaa gcgcgacaat
     2461 ctggagatca gccccaattt atgggcgggc gttggcttag tgcgcggcgg tgccgggacg
     2521 gcgctggtgg gcgatggtcc tacggtcgct gcgcgaatca acgaatatgc cgcgcttggc
     2581 atcgacagct ttgtgctttc gggctatccg catctggaag aagcgtatcg ggtcggcgag
     2641 ttgctgttcc cacatctgga tgtcgccatt ccggaaattc cccagccgca gccgctgaat
     2701 ccgcaaggcg aagcggtgga gaatgatttt atccctcgca gagtcgcgca aagctaagga
     2761 agaagataat ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc tggtttttac
     2821 cggtgggcat cgtgacggtg tggcaactgg cctcctcggt tggctggttg tcgacgcgta
     2881 ttttgccctc accggaaggg gtggtgatgg cgttctggac gctctcagcc agcggcgaat
     2941 tgtggcagca tctggcgatc agctcctggc gggcgctgat tgttttttca attggcggct
     3001 cgctggggtt gattttggga ctgattagcg gactgtcgcg ctggggagaa aggctgctgg
     3061 atacctcaat tcagatgttg cgcaacgtgc cgcatctggc gctgatcccg ctggtgattt
     3121 tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt ggcgctcggt acgctattcc
     3181 ccatttatat caacacctgg catggcatcc gtaatatcga tcgcgggttg gtggagatgg
     3241 cgcgtagcta tggattatcc ggcataccgc tgtttatcca tgtgatcctg cctggtgccc
     3301 tgccctcaat tatggtcggc gtgcgttttg cgttgggcct gatgtggctg acgctgattg
     3361 ttgccgaaac catttctgcc aattcaggca ttggttatct ggcgatgaat gcgcgggagt
     3421 ttttgcaaac ggacgtggtg gtagtcgcta ttattcttta cgccctgctc ggcaaacttg
     3481 ctgacgtcag cgcgcagctg ttagagcgcc tctggttgcg ctggaacccg gcttatcatt
     3541 tgaaggaggc cacggtatga atactgctcg tctgaaccag ggcacaccat tgttgctcaa
     3601 tgcagtaagc aaacattacg cggaaaatat cgtcctgaac caactggatt tacatattcc
     3661 ggcaggtcag tttgtggcgg tggtgggccg cagcggtggt ggcaaaagta ccctgctgcg
     3721 cctgctggca ggtctggaaa cgccaaccgc tggcgatgtg ttagcgggca ccacaccgct
     3781 ggctgaaatt caggaagaca cgcgaatgat gtttcaggat gcgcgtctgc taccgtggaa
     3841 atcggtgatt gataacgttg ggttaggcct taaaggccag tggcgcgatg ccgcgcgtcg
     3901 ggcgttggct gcggtaggac tggagaatcg cgccggggaa tggcctgccg cactttctgg
     3961 cgggcagaaa cagcgagtgg cgctggcaag agcgttgatt catcgaccgg gtttattgtt
     4021 gcttgatgaa ccgctcggtg cgctggatgc cttaacgcga ctcgagatgc aggatttgat
     4081 tgtgtcactt tggcaggagc acggttttac cgtactgttg gtgacgcatg atgtgagcga
     4141 agcggtagca atggctgatc gggtgttgtt aattgaagag ggaaaaattg gtctggattt
     4201 gacggtggat attccccgtc cgcgccggtt agggtcggtg cgattagcgg agctggaagc
     4261 ggaagtgttg caacgggtga tgcagcgtgg tgaatctgaa actcgcctga gaaaacaggg
     4321 gtaaaatccc cgaatggcgg cgctattgcc gccattcggg gatttattaa gccagtgctt
     4381 tggtgatctt ctcgtacaga tcgccagata gattttccag ccctttcaac tgttccagcg
     4441 ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc agcggttcaa
     4501 tcaggcgtga agctacctgc gggttacggc tgttcaggtc ggtaagcatt tccaccagga
     4561 actggtaacc gctcccatct tcggcatgga acgctgccgg gttgctgccc gcaaacgcac
     4621 caatcaacga acgaatgcgg ttcgggttgc tcatggtaaa tgagcgatgc tgcaacaggc
     4681 cgcgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata aaccatttat
     4741 ccatcaccag accatcctga tgccacttgt cgtcgtactc ctgcatcagc gcgtcacggc
     4801 aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc atattgtttg
     4861 cttcatggta ttgcttgctc accagcacgt cagccagatg cgtttcacca aaagcaagga
     4921 agcgcaggca ggcattacgc agagtgcgtt ttgcaatatc ttcatgctca acacggtact
     4981 cgctctggta attcgcgttg taaatagcca gtagctcatc cgccagttca gtcgccagag
     5041 tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata tcgaacagtt
     5101 cagccatttc attgacagaa ggcagcgtca ggatttctgc cgccagcgcc ggatcaatct
     5161 tctcatcgag cagcaccgcg cggaaagcgt cagccacatg taccggcaga gacagcggct
     5221 gcccttgctg atgacgggcg acgttcagct tgatgtaggt tgccagcaaa ctttgcgccg
     5281 catcccaacg ggaaaaatca ttacgcgcat gacgcatcag gaaggtcagt tgctgatcgc
     5341 tccacttata ttccagtttc actggcgcag agaattcgca cagcagcgca ggcaccggct
     5401 ggaagtagac attatcaaaa acaaaggtct gttccgcctg ggtgacgttc agcacggaat
     5461 tcaccggatg accgcctttc tgcaacggga tcactttgcc ttcgttgtca tacaattcga
     5521 tggcaaacgg aatatgcagc ggctgttttt ctgcttgatc cggcgtggct ggcgtgcgct
     5581 ggctgatggt cagggtgtac tgctcggttt ccggattgta gtcgtctttg acggtcacaa
     5641 ccggcgtacc ggactggctg taccaacggc ggaaatggga gagatcgaca ttcgacgcat
     5701 cttccatcgc ctgaacgaaa tcgtcgcagg tcgctgcact gccatcatgg cgctcgaaat
     5761 agagctgcat ccctttctgg aagttttctt cgccaagcag agtgtggatc atacgaatca
     5821 cttccgcgcc cttctcgtaa acggtcaggg tgtagaaatt gttcatctca atgaccatgt
     5881 ccgggcggat tgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat ccgcgcatgg
     5941 tgcgcacatt attgatacgg tttaccgcgc gggaaccaag gtcagagctg aattcctgat
     6001 cgcggaagac ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg caggtcactc
     6061 ggttaccggt ccagttatgg aaatattcat ggccgataac gcgttcaata tcgaggtaat
     6121 ctttgtcggt ggcggtatcg gtgcgggcca gcacatattt ggagttaaag atattcagac
     6181 ccttattctc cattgcgccc atattgaaga aatccaccgc gacgatcata tagatgtcga
     6241 ggtcatactc caggccaaag cgttcttcat cccatttcat ggagtttttc agcgaggtca
     6301 tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta cagctccagt gctacttcgc
     6361 gaccagaacg cgtggtaaag gtatcgcgca gtacatcaaa gtcgcctgcc accagcgcaa
     6421 acaggtagca cggtttcggg aacgggtcct gccactgtac ccaatggcgt ccgttttcca
     6481 gttcgccttg cgcaacgcgg ttaccgttgg aaagcaggaa gggatatttg gttttatcgg
     6541 caataatttt ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa tacgtaatat
     6601 ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc gcctgactga taaagccctt
     6661 ccagcgccgt attcgccgcc gggcttattt cattaatgat cttaagcgta aaacgctccg
     6721 gcaaattact gatgaccagt gcgccctctt cttctttcca ggcggtccac ggctcatcat
     6781 taatatgaac agaaaccagt ttgaggtctt cgccgttgag acaaagcgga gcatctgatg
     6841 caccatgacg gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg tcgaggtcaa
     6901 aggtcaagtc aatatcagta atctggtaat ccggcgcacg ataatcgtga cggtatttgg
     6961 cttgtggctg ttgagtcata aataaccttt agcatctttt atagagtctg gtgttcagtc
     7021 tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta caaacgcctt
     7081 tattgctaca tttttataac atacaccgcg taatgccatc gaccagaaag gtggcatatg
     7141 gtgtgatcgg ggttcaataa attgcgaaac aaggtatact ccagcagttc ctgaagatgt
     7201 ttattgtact aaacgctcct gtacgaggac gctactgcgc acctatgaca caattcgctt
     7261 ctcctgttct gcactcgttg ctggatacag atgcttataa gttgcatatg cagcaagccg
     7321 tgtttcatca ttattacgat gtgcatgtcg cggcggagtt tcgttgccga ggtgacgatc
     7381 tgctgggtat ttatgccgat gctattcgtg aacaggttca ggcgatgcag cacctgcgcc
     7441 tgcaggatga tgaatatcag tggctttctg ccctgccttt ctttaaggcc gactatctta
     7501 actggttacg cgagttccgc tttaacccgg aacaagtcac cgtgtccaac gataatggca
     7561 agctggatat tcgtttaagc ggcccgtggc gtgaagtcat cctctgggag gttcctttgc
     7621 tggcggttat cagtgaaatg gtacatcgct atcgctcacc gcaggccgac gttgcgcaag
     7681 ccctcgacac gctggaaagc aaattagtcg acttctcggc gttaaccgct ggtcttgata
     7741 tgtcgcgctt ccatctgatg gattttggca cccgtcgccg tttttctcgc gaagtacaag
     7801 aaaccatcgt taagcgtctg caacaggaat cctggttcgt gggcaccagc aactacgatc
     7861 tggcgcgtcg gctttccctc acgccgatgg gaacacaggc acacgaatgg ttccaggcac
     7921 atcagcaaat cagcccggat ctagccaaca gccagcgagc tgcacttgct gcctggctgg
     7981 aagagtatcc cgaccaactt ggcattgcat taaccgactg catcactatg gatgctttcc
     8041 tgcgtgattt cggtgtcgag ttcgctagtc ggtatcaggg cctgcgtcat gactctggcg
     8101 acccggttga atggggtgaa aaagccattg cacattatga aaagctggaa attgatccac
     8161 agagtaaaac gctggttttc tctgacaatc tggatttacg caaagcggtt gagctatacc
     8221 gccacttctc ttcccgcgtg caattaagtt ttggtattgg gactcgcctg acctgcgata
     8281 tcccccaggt aaaacccctg aatattgtga ttaagttggt agagtgtaac ggtaaaccgg
     8341 tggcgaaact ttctgacagc cctggcaaaa ctatctgcca tgataaagcg tttgttcggg
     8401 cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc cagttaatat catcagggag
     8461 ctaatcggct cccttttttt acctttaatt ccgaaatctt tcgctgcatt tgcgaattct
     8521 gcttgtctga ttgcagatgc caggtaacat aggtatcccc ccatttacgg gtggacaagt
     8581 gtttattttt tccgactatt aacagagaga atattatgag cgttgtgcct gtagccgacg
     8641 tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc
     8701 gccgagattc aaaagctggc atctccttcc tcgccgttta tgacggttcc tgctttgatc
     8761 ctgtacaggc tgtcatcaat aattctctgc ccaattacaa tgaagacgtc ctgcgtctga
     8821 ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc gtcgccgggc caggggcaac
     8881 aatttgaaat tcagaccagc aaggttgaag ttgctggttg ggttgaagat ccagacactt
     8941 acccgatggc ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct cacctgcgtc
     9001 cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc
     9061 atcgcttctt taacgagcag ggattcttct gggtttcaac gccactgatt actgcgtctg
     9121 ataccgaagg ggctggcgaa atgttccgcg tttctacgct ggatctggaa aatctgccgc
     9181 gtaacgatca gggcaaagtg gatttcgaca aagacttctt tggtaaagag tctttcctga
     9241 ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc attatccaaa atttatacct
     9301 tcggcccgac tttccgtgct gaaaactcca acaccagccg tcacctggcg gaattctgga
     9361 tgctggagcc ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg gctgaagcca
     9421 tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc tgacgacatg aaattcttcg
     9481 ctgaacgcgt agataaagat gccgtttcac gtctggaacg ctttattgag gccgatttcg
     9541 cgcaggtgga ttacaccgaa gcagtaacca ttctcgaaaa ctgcggcagg aagtttgaaa
     9601 acccggttta ctggggcgtc gatctctctt ctgagcatga gcgttatctg gcggaagaac
     9661 actttaaagc accggtagtg gttaaaaact atccgaaaga tattaaagcg ttctatatgc
     9721 gcctcaacga agacggtaaa accgttgcgg ctatggatgt tctggctccg ggcatcggtg
     9781 agatcattgg tggctcccag cgtgaagagc gtctggacgt gctggacgag cgtatgctgg
     9841 aaatgggcct gaacaaagaa gattactggt ggtatcgcga tctgcgtcgc tacggtactg
     9901 ttccgcattc cggtttcggt cttggttttg aacgcctgat tgcttacgta actggtgtgc
     9961 aaaacgtgcg tgatgtgatt ccgttcccac gaactccgcg taacgccagc ttctaattta
    10021 gcgtctacaa gagccagcac ccgctggctc ttttttatcc tttctgtcaa gttatctgtt
    10081 tgttaagtca atcaatctat ttgcaacccc gccataacga tcatcctgtt acggaatatt
    10141 acattgcaac atttacgcac aaaaaataac ccgcattctt attgcgg
//
LOCUS       DAZFKY010000168        10156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFKY010000168 DAZFKY010000000
VERSION     DAZFKY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            162..314
                     /gene="hokA"
                     /locus_tag="ACHKCL_003786"
     CDS             162..314
                     /gene="hokA"
                     /locus_tag="ACHKCL_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5764669.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(291..362)
                     /locus_tag="ACHKCL_003787"
     CDS             complement(291..362)
                     /locus_tag="ACHKCL_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764670.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(502..714)
                     /gene="cspA"
                     /locus_tag="ACHKCL_003788"
     CDS             complement(502..714)
                     /gene="cspA"
                     /locus_tag="ACHKCL_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5764671.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(995..1285)
                     /gene="yiaG"
                     /locus_tag="ACHKCL_003789"
     CDS             complement(995..1285)
                     /gene="yiaG"
                     /locus_tag="ACHKCL_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5764672.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1719..2429
                     /gene="yiaF"
                     /locus_tag="ACHKCL_003790"
     CDS             1719..2429
                     /gene="yiaF"
                     /locus_tag="ACHKCL_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5764673.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2479..3453)
                     /gene="ghrB"
                     /locus_tag="ACHKCL_003791"
     CDS             complement(2479..3453)
                     /gene="ghrB"
                     /locus_tag="ACHKCL_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5764674.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAVLLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3557..4216)
                     /gene="yiaD"
                     /locus_tag="ACHKCL_003792"
     CDS             complement(3557..4216)
                     /gene="yiaD"
                     /locus_tag="ACHKCL_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5764675.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4369..6702
                     /gene="bisC"
                     /locus_tag="ACHKCL_003793"
     CDS             4369..6702
                     /gene="bisC"
                     /locus_tag="ACHKCL_003793"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5764676.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6671..7111)
                     /gene="yiaC"
                     /locus_tag="ACHKCL_003794"
     CDS             complement(6671..7111)
                     /gene="yiaC"
                     /locus_tag="ACHKCL_003794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5764677.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7108..7671)
                     /gene="tag"
                     /locus_tag="ACHKCL_003795"
     CDS             complement(7108..7671)
                     /gene="tag"
                     /locus_tag="ACHKCL_003795"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5764678.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7829..8527
                     /gene="yhjY"
                     /locus_tag="ACHKCL_003796"
     CDS             7829..8527
                     /gene="yhjY"
                     /locus_tag="ACHKCL_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5764679.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8756..9958
                     /gene="yhjX"
                     /locus_tag="ACHKCL_003797"
     CDS             8756..9958
                     /gene="yhjX"
                     /locus_tag="ACHKCL_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5764680.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
     gene            complement(9966..>10156)
                     /locus_tag="ACHKCL_003798"
     CDS             complement(9966..>10156)
                     /locus_tag="ACHKCL_003798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764681.1"
                     /translation="KIIALHFHIKSYRLFLGINKYKIPIKQNKNIHHANNKIKYFHFY
                     SLKCNKKGGFPPPFVQNL"
BASE COUNT         2432 a         2447 c         2598 g         2679 t
ORIGIN      
        1 tagaatagcc acgggtgctt gagactgttt gtctcaggta ttcaccgaaa ggcagacaga
       61 gaaaagcccc acctgactat aaatcaaagt gaggcccctc tatgtataga caccataagg
      121 ttagcctctt actcgttgga aatcaacaca gggggctggg aatgccgcag aaatatagat
      181 tgctttcttt aatagtgatt tgtttcacgc ttttattttt cacctggatg ataagagatt
      241 cactgtgtga attgcatatt aaacagggga gttatgagct ggcggcattt ttagcctgca
      301 atttgaaaga gtaagagtct tcggcggaaa gttattcccg cctttcttac ggcgttgcgc
      361 attctcattg cacccaaatt tatttttcac aaaaataata ctagatttta ttacgcgatc
      421 gattatttat ttcctgaaaa acaaataaaa aaatccccgc caaatggcag ggatcttaga
      481 ttctgtgctt ttaagcagag attacaggct ggttacgttg ccagctgccg ggcctttagc
      541 gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt aaccatcgtt
      601 ctggatagca gagaagtgta cgaacacgtc tttagagcca tcgtcaggag tgatgaagcc
      661 gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg acatagtgta
      721 ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag attttgaagc
      781 gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga cttgccttac
      841 tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca cgcattggcg
      901 ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg gtcctgtcat
      961 caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc ttacttaatg
     1021 ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc ttcacgcgtc
     1081 tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg aaatcatcga
     1141 tttttaatcc tgtccctttt cgtaactgct caatttcggt acaggacgtt gttctgtgcg
     1201 tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg ctcaacagct
     1261 catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg ggatcgtgaa
     1321 ttgcatcgaa gctcattaag aatagtcggg aaaagcagac acagcggggt gaagtcaggg
     1381 catgctctgg ctatgattcg attcagccag tcatttagcg tgacttatag ccacctaacg
     1441 atctgataaa tgccgatata tctctaacca ctcctggttt gtaaagattt cgaaaatcta
     1501 accttctgcg aaggcgtaaa tctctggcta caaaaaaggc aacggcaata tcatttttta
     1561 acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca ggaagattac
     1621 gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt
     1681 gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct
     1741 ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt gggtgttttg
     1801 ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca gtgatgcgta
     1861 gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt ccttttgtct
     1921 ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg gattccggtc
     1981 tgcgcccggt tgtagacagc gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa
     2041 gcggtccgct gcgtgaaatg aatggttcgc tgggtgtgct ggcgcaacaa ctgcaaaatg
     2101 cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagtgatgac ctgaaaccgg
     2161 tctttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg caaccgttga
     2221 ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac attgcccaac
     2281 aaggtactca ggtgagcttt gtagcaaatg gcatccagtt cccgacttca cagcaggcaa
     2341 gtgaatataa caaactgatt gcgccattac cggcacaaca tcaggcgttt aatcaggcct
     2401 ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt atcgggcttt
     2461 actccacgca gtcgcggctt agtccgcgac gtgcggattc acacagttct tctcaacctt
     2521 tccttgtaac gcatcaatca aattatccac ggcacaggcg gccatgccat aacgcgtctc
     2581 atgggtggca gatccaatat gcggtactgc gacgacgttg gccattgaga gcaacggcga
     2641 atctacggaa agtggctctt gttcgaagac atccagcccg gcggcgtgaa tttccccttt
     2701 ctgcaatgct gcgatcagtg cattttcgtc aaccaccggg ccacgtccgg cattaatgaa
     2761 aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat gcgtctcatc
     2821 agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagtgtat ccaaatcgca
     2881 gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt tatagaggat
     2941 gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc ataccgatcc gtcccatccc
     3001 gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc
     3061 ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag cggtagacaa
     3121 caccagcgcc atcagcgtat cggcgacggt ttctgttaag acggttggcg tgtgcatcag
     3181 cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga cggagatcgt
     3241 tgatgtggca cgcagtttcg gcattttttc cagcaatacg gcatcaacat tctcgtttga
     3301 acccagtaaa ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct
     3361 gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg
     3421 taaggctttg tagaggataa cggacggctt catgcttctc tccattcact gataaggcga
     3481 tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat ttctgcatca
     3541 cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg ttttgcgcct
     3601 taccttctgc ggtgctgttg ctggcgattg ggttagccgg gccaaggccc tgagtacgga
     3661 tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc gcacgctgct
     3721 gggagagacg catgttcaga tcgtgaccac ccgtgctgtc agtataacca atcacgttaa
     3781 ccgccgtttt cggatactct ttcagcacca ttgccacgcc ggtcagggtg ttagcgcccg
     3841 ccggtttcag ggttgcgctg ctgctgtcga aggtcacatt gttcggcata ttgaggataa
     3901 tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca cgcagcttcg
     3961 cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcgcct gctgctgcgc
     4021 caatcagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca atacccgcgc
     4081 ccacgagaga gcccagacct gcgccgatag cagatttacc tgcttcgcgt tcgccggtgt
     4141 aagggttagt tgtgcagcca gataccgcca gagcgccact cactacggcg gcaataagat
     4201 aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca cgggttccgt
     4261 ggcgggagat tatgccgcgt gaacatgaag attattcctg ggaatactcg gaaatttgta
     4321 agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt gaccaactca
     4381 tcctcacgat attccgttct gactgccgcc cattgggggc ccatgctggt tgaaaccgac
     4441 ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag
     4501 agcgcggttc gtgaccaggt tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa
     4561 ggctttctcg cgtcaccgga aaacccgcaa ggcattcgtg ggcaggatga atttgttcgc
     4621 gtgagttggg atgaggcgct ggatcttatt caccaacaac ataaacgcat tcgtgaggct
     4681 tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg cgtgctgcat
     4741 aaggcctcga cattattaca acgctatatg gcgctggcag gcggttatac cgggcatctg
     4801 ggggattatt cgaccggcgc ggcacaggcg atcatgccgt atgtcgtggg tggtagtgaa
     4861 gtttatcaac agcagaccag ttggccgctg gtgctggaac atagcgatgt cgtggtgctg
     4921 tggagtgcta acccactcaa tacgctgaaa attgcgtgga atgcatccga tgagcagggg
     4981 ctttcttact tttctgcact gcgtgacagc gggaaaaagc tgatctgcat tgatccaatg
     5041 cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt gggtggcacc gcacatgggc
     5101 actgatgttg cgctgatgct ggggatcgcc catacgctgg tggaaaatgg ttggcacgac
     5161 gaagcgtttc tggcgcgttg caccaccggt tatgccgtct tcgcctctta tttgctgggc
     5221 gagagtgacg gaatagcgaa aaccgccgaa tgggcagcag agatttgtgg tgttaacgca
     5281 gagaaaatcc gcgagctggc ggctattttc caccaaaata ccaccatgct gatggctggc
     5341 tggggaatgc agcgccaaca gtttggtgag caaaaacact ggatgatcgt cacgctggca
     5401 gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta ccattttgcc
     5461 aatggtggta accccacgcg gcgcgctgcg gtactctctt ccatgcaggg cagcttgccg
     5521 ggtggctgcg atgcggtaga taaaatccct gtcgcccgca ttgttgaagc actggaaaac
     5581 cccggtggcg catatcaaca caacggtatg gaccgacatt tcccggatat tcgttttatc
     5641 tggtgggcgg gcggtgccaa ctttactcat catcaggata ccaatcgcct gatccgtgcc
     5701 tggcaaaaac cggagctggt ggtgatctct gaatgcttct ggacggcggc ggcaaaacac
     5761 gcggatatcg ttctgcctgc gactacctct tttgagcgta atgatctcac catgaccggt
     5821 gattacagta atcagcatct ggtgccgatg aagcaagtgg tgccgccacg ctatgaagcg
     5881 cgtaatgatt ttgatgtttt tgccgagtta agtgaacgct gggagaaggg cggttatgca
     5941 cgttttacgg aaggaaaaag tgagctgcaa tggctggaaa cgttttataa cgttgcccgg
     6001 cagcgcgggg caagccagca ggttgaattg ccgccattta ctgagttctg gcaagccaac
     6061 cagttaattg agatgccgga aaacccggac agcgagcggt ttattcgctt cgctgatttt
     6121 tgccgcgatc cgcaggcgca tccattaaaa accgccagcg gtaagattga aatcttctcg
     6181 cagcgtattg ccgattacgc ttatccggat tgcccggggc atccaatgtg gctggtgccg
     6241 gacgaatggc agggcaatgc cgaaccggaa cagttacagg tactttctgc tcatccggcg
     6301 catcgtctgc acagccagct gaattacagt tctctgcgcg aattgtacgc ggtggcaaac
     6361 cgtgagcctg tcaccattca tcctgacgat gcccaggcgc gcggcataca agatggcgat
     6421 actgttcggc tgtggaacgc acgcgggcaa attcttgccg gagcggtcat tagcgaggga
     6481 attaaacctg gcgtgatttg cattcacgaa ggggcatggc cggatctgga tttaaccgct
     6541 gacggtattt gtaaaaacgg cgcagtgaac gtgctgacca aagatctccc cagctcgcgg
     6601 ctggggaatg gctgtgcggg taatacggcg ctggcatggc tagaaaaata caacggtccg
     6661 gaactgacac ttacagcgtt tgaaccaccg gccagctcat aatccaggtg ggtagttggg
     6721 tttcatcctg ccatgcgcaa tcgacaatgt gaaaaccctg cgcctggtaa aaatctatcg
     6781 ccggttgatt tttttgataa acctccagca tcagatgggg atagcgctgc tgcacatact
     6841 gcatcagcgc cttaccaata ccgcgtctga cggccttcgg tgcgacaaac attgctgcca
     6901 gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct tcccagaccc
     6961 agtttttcgc attggcaaga tacgcatccc gcaccagcgg aatgcactcg cgccagtaat
     7021 tcacttttat aaagggatgc ccccaggttg tactttccag ccacagttcg aggatcgcgg
     7081 ggagttctga acgttgcgct tcccgaatca tggtttattt cccggatagc aacagcagcc
     7141 aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga ttgtggtacc
     7201 gacaaactta aaaccacgtt ttttcagtgc cttagatagg gcgtcggagg cgggcgtaga
     7261 tgtggggatt tcgctcaacg ttgtggcttg tgtcacctgt ggctgatgat ttacaaacga
     7321 ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt
     7381 accaataatt gcctgaattt tcccgcgatg gcggataatc ccggcgtcct gtaccagtct
     7441 ttcgacatcc tcttcctgca ttgcggcaac cttcaccgga tcgaactgat gaaagcaggc
     7501 gcgatagttt tcgcgttttt tgaggacggt gatccacgat aacccagcct gctgcccttc
     7561 caggcagatc atttcgaaca gttttttcct gtcagtttca ggcacgcccc actcattatc
     7621 atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca tactttccct
     7681 cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc
     7741 aatatttaat acagggctac tatgcccgat atccttcata caatgacaat aatattgccg
     7801 aattcggctc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca
     7861 gctaagcctg ctggcgagtg tggtaatcag tgcctttttt ctcaacacag cttacgcctg
     7921 gcaacaagaa tatatcgttg atacgcaacc cgggcattcc acagagcgtt acacctggga
     7981 tagtgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag
     8041 ggcgctggga ctggaagtca atctggcgga agaaacccct gtggatgtga ccagcagtat
     8101 gagtatgggc tggaattttc ctttatatga acaggttaca actggcccgg tcgcggcatt
     8161 acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta ctaccacgct
     8221 gaccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg actcccggct
     8281 tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg agaatatctg
     8341 gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca actggctgga
     8401 tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gccgcctatg ccgcgctatc
     8461 tcaggcagaa aataccacta acaatagcga ttatctgtat acgatggggg ttagtgccag
     8521 attttaacgt aacaatcaca attgaaacca ttgaataaca atagttgtgg cgatagtggg
     8581 tgctaactta ccaaataata aatttggtga ataattgtcg cgtcattcat tcctgaacta
     8641 aggcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca taaagtgcac
     8701 ttcgttatgg ttgtcggcag agatttttcc tttttattac tgcaggaata ctgccatgac
     8761 accttcaaat tatcagcgta cccgctggtt gacactcatc ggtactatca ttacccagtt
     8821 tgcgctgggg tcggtttata cctggagcct gtttaacggc gcgctttccg ccaagctgga
     8881 tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc tggcaatatc
     8941 gtcttctgtt gcgggcaaat tgcaggaacg ttttggtgtt aaacgcgtca ccatcgcttc
     9001 cggcattttg ctgggattag ggttcttcct gacagcgcat tctgacaacc tgatgatgct
     9061 gtggttaagc gccggtgtgc tggtcggtct ggcggatggc gcgggttacc tgctgacgct
     9121 ctctaactgc gtgaaatggt tcccggagcg taaaggtctg atctccgcgt tcgctatcgg
     9181 ttcttatggt ctgggtagcc tgggtttcaa atttatcgac acgcagctgc tggaaacggt
     9241 cggtctggaa aaaacctttg tgatttgggg agcgattgcg ctggtgatga ttgttttcgg
     9301 cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg gtgtggtgga
     9361 gaaagattac acgctggcag agtcgatgcg taaaccgcag tactggatgt tagcggtaat
     9421 gttcctgact gcctgcatga gcggcctgta cgtgattggg gtagcgaaag atatcgccca
     9481 aagtctggca caccttgatg tggtttctgc agccaatgca gtcactgtta tttccatcgc
     9541 caacctttca ggtcgtctgg tgctgggcat tctgtctgac aaaatcgccc gtatccgtgt
     9601 tatcaccatt ggtcaggtaa tttcgctggt gggtatggcg gccctgctgt ttgcaccatt
     9661 gaatgcagtg acgttctttg cagcgattgc ctgcgtggca tttaactttg gcggcactat
     9721 taccgtcttt ccgtcactgg tcagtgagtt cttcggcctc aataacctgg cgaaaaacta
     9781 cggtgtgatt tacctcggtt tcggtatcgg tagcatttgt ggttcgatta tcgcctcact
     9841 gtttggcggc ttctatgtga agttctacgt catctttgct ctgctgattc tgtcattggc
     9901 gctttctacg acgattcgtc agccagagca gaaagtattg cgtgaggcgc atatttagcc
     9961 agaaactaca ggttttgaac gaaaggggga ggaaatcctc cttttttatt gcattttaaa
    10021 gaataaaaat gaaaatattt tatcttatta tttgcgtggt gaatgttttt gttttgttta
    10081 attggtattt tatatttatt tattcctaaa aataaacgat agctttttat gtgaaaatgt
    10141 aatgcaatga tttttt
//
LOCUS       DAZFKY010000169        10124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFKY010000169 DAZFKY010000000
VERSION     DAZFKY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..742
                     /gene="yahL"
                     /locus_tag="ACHKCL_003799"
     CDS             <1..742
                     /gene="yahL"
                     /locus_tag="ACHKCL_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO5764682.1"
                     /translation="NQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKLIH
                     LMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFRILENQKGTVWVYDQ
                     DDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEHHMPLLRLFPKGKECMHVE
                     KWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDDARL
                     NEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1194..1403
                     /locus_tag="ACHKCL_003800"
     CDS             1194..1403
                     /locus_tag="ACHKCL_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764683.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1420..2091)
                     /gene="yahN"
                     /locus_tag="ACHKCL_003801"
     CDS             complement(1420..2091)
                     /gene="yahN"
                     /locus_tag="ACHKCL_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5764684.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2238..2513
                     /gene="yahO"
                     /locus_tag="ACHKCL_003802"
     CDS             2238..2513
                     /gene="yahO"
                     /locus_tag="ACHKCL_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5764685.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(2611..4197)
                     /gene="prpR"
                     /locus_tag="ACHKCL_003803"
     CDS             complement(2611..4197)
                     /gene="prpR"
                     /locus_tag="ACHKCL_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5764686.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            4436..5326
                     /gene="prpB"
                     /locus_tag="ACHKCL_003804"
     CDS             4436..5326
                     /gene="prpB"
                     /locus_tag="ACHKCL_003804"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5764687.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            5482..6651
                     /gene="prpC"
                     /locus_tag="ACHKCL_003805"
     CDS             5482..6651
                     /gene="prpC"
                     /locus_tag="ACHKCL_003805"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5764688.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6685..8136
                     /gene="prpD"
                     /locus_tag="ACHKCL_003806"
     CDS             6685..8136
                     /gene="prpD"
                     /locus_tag="ACHKCL_003806"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5764689.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8176..10062
                     /gene="prpE"
                     /locus_tag="ACHKCL_003807"
     CDS             8176..10062
                     /gene="prpE"
                     /locus_tag="ACHKCL_003807"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5764690.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         2481 a         2595 c         2712 g         2336 t
ORIGIN      
        1 caatcagtta ccagaggata tcaaaagctc cctggtttcc tgtgtcgata tattcaaggt
       61 tttgattaaa caatattatg attatcctta tgattgtcgt gatgatttag tcgatgacga
      121 caaactcatt catctcatgg ctgccgtacg agactgtgag tggtcagacg ataacgcact
      181 caccataaat gtgcagttta atgatttccc tggattttat gactggatgg attaccctga
      241 tcacccggtt aagtttgttt ttcgcatact tgaaaatcaa aaagggacag tttgggtata
      301 tgaccaggat gatgcgtttc ttgatattaa agcaaatgtg caggctggac gctttaccgg
      361 gcttaagaaa ttagtgcagt ttatcaattc agtgcgaaca gattgcaaat gtattcttct
      421 tgaacaccat atgcctttgc tgagactttt ccctaaaggg aaagagtgta tgcatgtgga
      481 aaaatggctt cgcgaaatgt cgtctattcc ggagacagac gcgcccatta agcaggcgct
      541 tgcgcatgga ttattattac acttaaaaaa tatctaccct gtttttcctg aatcactggt
      601 gatgttattg ctctctgttc tggacgtaaa aacatacaga gatgatgcac gattaaatga
      661 agggatatct aatagagtcc aggaattagg tgacagatat tatcctgtaa ataagcacgt
      721 taaaatccgc tacactcttt agttcagaaa gggttccacc tgcaagttgt tattctacct
      781 gagagtgaat cgcaatgcag gtaatcattt catctgtata tctgtatgcc aactggcatt
      841 ccatctcttt tttcgccgat attttgttgt gatcggcgac acttcgctta aaaaagcacc
      901 agtagtggtt tcgcagccat gcggtgtata aaaaaaaatg atctcatgca gatgttttgt
      961 gaatgtgtgg gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt
     1021 cttttctgta tcttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata
     1081 tcaaaaaccg ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta
     1141 cggggttcga ggccccgatg gcggtccaac ttttcaaaac tcttctgaat tagatcccct
     1201 tattatcatc cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac
     1261 caatgaaaaa agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta
     1321 aacttatcgg aaataaggac cacgcattac gggggctatc atcgccgatg gtcgggattc
     1381 ggcaaatcag gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc
     1441 gtaaatcagg cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg
     1501 ttgcatacgc ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaaaac
     1561 tcgccagata attgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg
     1621 tgtttcggca tttaatgtta ctgagaaaat actaataaaa aataaaacgg tttgtgggtt
     1681 agagagatcg gtaattaatc cacggcgaaa aaagacatac cagggggcgt taatcggttg
     1741 ttgtagtgtg ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa
     1801 gagataagcg ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgtaattag
     1861 cgttgcaaga ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag
     1921 tccggtcagc actcctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaaaag
     1981 attggctccc ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcatg
     2041 caaaggatcc atagtgattt catccataaa taagtgaact aactgcatca tatttctacc
     2101 aaaaataatc gggtgcgaga gagatcacaa agtgtcttat ttccggttac tggcgtttat
     2161 gccctgactg aactaattat taatcaaccc aataatgtcg gtgggtgata gtgtgataac
     2221 aactctggag ccgtaatatg aaaataatct ctaaaatgtt agtcggtgcg ttagcgtttg
     2281 ccgttaccaa tgtctatgcc gctgaattga tgaccaaagc ggaatttgaa aaagttgaat
     2341 cgcagtatga aaaaataggt gatatttcaa ccagtaatga aatgtcgact gcagatgcaa
     2401 aagaagattt aatcaaaaaa gccgatgaaa aaggggctga tgtgttggta ctgacctccg
     2461 gtcaaactga caataagatc cacggcacgg cggatattta taagaagaag taattctgaa
     2521 tcctatgtaa acatccccga tgcgtaagtt tatcggtgat catctattga aatttatgcc
     2581 ggataaagcg ttcgcgctgc attcggcagt tcagcttttc agccgccgcc agaacgtcgt
     2641 ccggctgatg cctaaataat tcgccgctgc tgttttatcg ccattaaatt tctccagtgc
     2701 ctgttgtggt gtcagcaagc gtggagcggg aattttcgcc gactcgcgcg ccagttccgg
     2761 cagtagcagc tgcaaaaact gcggcgttaa atccggcgtc ggttccacac ttaaaaatag
     2821 cgccagtcgc tccatcatat tgcgcagttc acgaatatta cctggccagt cgtagtgcac
     2881 cagcacggtt tcgcttgcct gtaatccctg gcgtaatgcg gcagaaaatg gggcggagag
     2941 cgccgccaga gacactttca aaaagctttc cgccagcgga agaatatctg ccacccgctc
     3001 gcgcagtggt ggcaattgca gacgcaaaat actcagccga taaaacagat cacggcgaaa
     3061 ctgcccttgc cgcatatctt cttccagatt gcagtgagtg gcgctaatga cccgtacatc
     3121 taccggaaca ggctgatgcc cgccgacgcg ggtgacctct ttttcttcca gcacccgtaa
     3181 caggcgagtc tgcaaaggta gcggcatttc gccaatctca tccagaaaca gcgtgccacc
     3241 gtgggcaatt tcgaacagcc cggcgcgacc tccgcgtcgc gagccggtaa acgccccttc
     3301 ctcatagcca aacagctccg cttccagcag cgattcggca atcgccccgc agttgacggc
     3361 aacaaacgga tgcgactttt tgccctgtcg cgcatcgtgg cgggcaaaat attcccgatg
     3421 aattgcctgg gccgccagct ctttgcccgt ccccgtttcc ccctcaatca acaccgccgc
     3481 actggagcgg gcatacagca aaatagtctg ccgcacctgc tccatctgtg gtgattgacc
     3541 gagcatatcg cccagcacgt aacgagtacg cagggcgttg cgggtggcat cgtgagtgtt
     3601 atggcgtaac gacatgcgcg tcatatccag cgcatcgctg aacgcctggc gcacggtggc
     3661 ggcggaatag ataaaaattc cggtcattcc ggcttcttct gccagatcgg taatcagccc
     3721 cgcgccgacc accgcttcgg tgccgttagc ttttagctcg ttaatctgcc cgcgtgcgtc
     3781 ttcttcggta atgtagctac gttgatcgag gcgcaaatta aaggtttttt gaaacgccac
     3841 cagtgccgga atagtttcct gataagtgac aacgccgata gaagaggtga gttttccggc
     3901 ttttgccagt gcctgtaaca catcgtagcc gctcggttta atcaaaataa ctggcactga
     3961 caggcggctt ttcaggtacg cgccgttaga gccagcggcg atgatggcgt cacagcgttc
     4021 gtttgccagt ttcttgcgga tgtaggtcac tgctttttca aagccaagct gaataggggt
     4081 aatgttcgcc aggtgatcaa actcgaggct gatatcgcga aacagctcga ataagcgcgt
     4141 tacagatacc gtccagataa ccggtttgtc gtcattcagc cgtggtggat gtgccatagc
     4201 gcaccgcaaa gttaagatac cgaatattgg gtttagtctt gtttcataat tgttgcaatg
     4261 aaacgcggtg aaacattgcc tgaaacgtta actgaaacgc atatttgcgg attagttcat
     4321 gactttatct ctaacaaatt gaaattaaag atttaatttt attaaggcaa ttgtggcaca
     4381 ccccttgctt tgtcgttatc aacgaaaata acaagttgat aacaaaggat gggctatgtc
     4441 tctacactct ccaggtaaag cgtttcgcgc tgcactgact aaagaaaatc cattgcagat
     4501 tgttggcacc attaacgcca accatgcgct gttggcgcag cgtgccggat atcaggcaat
     4561 ttatctctcc ggcggtggcg tggcggcagg gtcgctgggg ctgcccgatc tcggtatttc
     4621 tactcttgat gacgtgttga cagatattcg ccgtatcacc gacgtttgtt cgctgccgct
     4681 gctggtggat gcggatatcg gttttggttc ttctgctttt aacgttgcgc gtacggtgaa
     4741 atcgatgatt aaagccggtg cggcgggatt gcatattgaa gatcaggttg gtgcaaaacg
     4801 ctgtggtcat cgcccgaaca aagcgatcgt ctcgaaagag gagatggtgg atcggatccg
     4861 cgcggcggtg gatgcgaaaa ccgatcctga ttttgtgatc atggcgcgca ccgatgcgct
     4921 ggcggtagag gggctggatg cggcgatcga gcgtgcgcag gcctatgttg aagcgggtgc
     4981 cgagatgttg ttcccggagg cgattaccga actcgccatg taccgccagt ttgccgatgc
     5041 ggtgcaggtg ccaatcctcg ccaacattac cgaatttggc gccacgccgc tgtttaccac
     5101 cgacgaatta cgcagcgctc atgtcgcgat ggcgctgtac ccgctttcag cgtttcgcgc
     5161 catgaaccgc gccgccgaac atgtctacaa cgtcctgcgc caggaaggca cgcagaaaag
     5221 cgtcatcgac accatgcaga cccgcaacga gctgtacgaa agcatcaact actaccacta
     5281 cgaagagaag ctcgacgacc tgtttgtccg tagccaggcg aaataaaaaa cgcccgttgg
     5341 ttgtattcga caaccgatgc ctgatgcgcc gctgacgcga cttatcaggc ctacggggtg
     5401 aactgtaggt cggataagac gcatggcgtc gcatccgaca acaatctcga ccctacaaat
     5461 gataacaatg acgaggacaa tatgagcgac acaacgatcc tgcaaaacag tacccatgtc
     5521 attaaaccga aaaaatctgt ggcgctttct ggcgttccgg cgggcaatac ggcgctctgc
     5581 accgtgggta aaagtggcaa tgacttgcat taccgtggct acgatattct tgatctggcg
     5641 gaacattgcg aatttgaaga agtggcgcat ctgctgatcc acggcaaact gccgacccgt
     5701 gacgaactcg ccgcctacaa aacgaaactg aaagccctgc gcggtttacc ggctaacgtg
     5761 cgtaccgtgc tggaagcctt accggcggcg tcacacccga tggatgttat gcgcaccggc
     5821 gtttccgcgc tcggctgcac gctgccagaa aaagaggggc ataccgtctc tggcgcgcgg
     5881 gatattgccg acaaactgct ggcgtcactt agctcgattc ttctctactg gtatcactac
     5941 agccacaacg gcgaacgcat ccagccggaa accgatgacg actccatcgg tggtcacttc
     6001 ctgcatctgc tgcacggcga aaagccgtcg caaagctggg aaaaggcgat gcatatttcg
     6061 ctggtgctgt acgccgaaca cgagtttaac gcctccacct ttaccagccg ggtgattgcg
     6121 ggcactggct ctgatatgta ttccgcgatt attggcgcga ttggtgcact gcgcgggccg
     6181 aagcacggcg gggcgaatga agtgtcgctg gagatccagc aacgctacga aacgccggac
     6241 gaagccgaag ccgatatccg caagcgggtg gaaagcaaag aagtggtcat tggttttggt
     6301 catccggttt ataccatcgc cgacccgcgc caccaggtga ttaaacgtgt ggcgaagcag
     6361 ctctcgcagg aaggcggctc gctgaagatg tacaacatcg ccgatcgcct ggaaacggtg
     6421 atgtgggaga gcaaaaagat gttccccaat ctcgactggt tctccgctgt ttcctacaac
     6481 atgatgggcg ttcccaccga gatgttcaca ccactgtttg ttatcgcccg cgtcaccggc
     6541 tgggcggcgc acattatcga acaacgtcag gacaacaaaa ttatccgtcc ttccgccaat
     6601 tatgttggac cggaagaccg ccagtttgtc gcgctggata agcgccagta aacctctacg
     6661 aataacaata aggaaacgta cccaatgtca gctcaaatca acaacatccg cccggaattt
     6721 gatcgtgaaa tcgttgatat cgtcgattac gtcatgaact acgaaatcag ctccaaagta
     6781 gcctacgaca ccgcacatta ctgtctgctc gacacgctcg gctgcggtct tgaagctctc
     6841 gaatacccag cctgtaaaaa actgctgggg ccaattgtcc ccggcaccgt cgtacctaac
     6901 ggcgtgcgcg tccccggaac tcagttccag ctcgaccccg tccaggcggc atttaacatc
     6961 ggcgcgatga tccgctggct cgatttcaac gatacctggc ttgcggcaga gtggggccat
     7021 ccttccgaca atctcggcgg cattctggca acggcggact ggctttcacg caacgcggtc
     7081 gccagcggca aagcgccgtt gaccatgaaa caggtgctga ccggaatgat taaagcccat
     7141 gaaattcagg gctgcatcgc gctggaaaac tcctttaacc gcgtcggcct cgaccacgtt
     7201 ctgttagtga aagtggcttc caccgccgtg gtcgccgaaa tgcttggcct gacccgcgag
     7261 gaaattctca acgctgtttc gctggcgtgg gtggacggtc agtcgctgcg cacctatcgc
     7321 catgcgccga acaccggcac gcgtaaatcc tgggcggcgg gcgatgccac ttcccgcgcg
     7381 gtacgtctgg cactgatggc gaaaacgggc gaaatgggtt acccgtcagc cctgactgcg
     7441 cctgtgtggg gcttctacga cgtctccttt aaaggtgaat cgttccgctt ccagcgtccg
     7501 tacggttctt acgtgatgga aaatgtgctg ttcaaaatct ccttcccggc ggagttccac
     7561 tcccagacgg cagttgaagc agcgatgacg ctctatgaac agatgcaggc agcaggcaaa
     7621 acggcggcag atatcgaaaa agtgtccatc cgcacccacg aagcctgtat tcgcatcatc
     7681 gacaaaaagg ggccgctcaa taacccggca gaccgcgacc actgcattca gtacatggtg
     7741 gcgatcccac tgctattcgg gcgcttaacg gcggcagatt acgaggacaa cgttgcgcaa
     7801 gataaacgca ttgacgccct gcgcgagaag atcaattgct ttgaagatcc ggtatttacc
     7861 gctgactacc acgacccgga aaaacgcgcc atcgccaatg ccattaccct tgagttcacc
     7921 gacggcacac gatttgaaga agtggtggtg gagtacccca ttggtcatgc tcgccgccgt
     7981 caggatggta ttccgaaact ggtcgataaa ttcaaaatca atctcgcgcg ccagttcccg
     8041 actcgccaac agcagcgcat tctggaggtt tctctcgaca gagctcgcct ggaacagatg
     8101 ccggtcaatg agtatctcga cctgtacgtc atttaagtaa acggcggtaa ggcgtaagtt
     8161 caacaggaga gcattatgtc ttttagcgaa ttttatcagc gttcgattaa cgaaccggag
     8221 cagttctggg ccgagcaggc ccggcgtatt gactggcaga cgccctttac gcaaacgctc
     8281 gatcacagca atccgccgtt tgcccgttgg ttttgtgaag gccgaaccaa cttgtgccac
     8341 aacgctatcg accgctggct ggagaaacaa ccagaggcgc tggcgctgat tgccgtctct
     8401 tcggaaacag aagaagagcg cacctttacc tttcgccagc ttcatgacga agtaaacgcg
     8461 gtggcgtcaa tgctgcgctc gctgggcgtg cagcgtggcg atcgggtgct ggtgtatatg
     8521 ccgatgattg ccgaagcgca tattactctg ctggcctgcg cgcgcattgg cgctattcac
     8581 tcggtggtgt ttgggggatt tgcctcgcac agcgtggcgg cgcgaattga tgatgctaaa
     8641 ccggtgctga ttgtctcggc tgatgccgga gcgcgcggtg gcaaaatcat tccctataaa
     8701 aaattgctcg acgatgcgat aagtcaggcg cagcaccagc cacgccatgt tttgctggtg
     8761 gatcgcgggc tggcgaaaat ggcacgcgtc agcgggcggg atgtcgattt cgcgtcgttg
     8821 cgccatcaac acatcggcgc gcgggtaccg gtggcgtggc tggaatccaa cgaaacctcc
     8881 tgcattctct acacctccgg cacgaccggc aaacctaaag gcgtgcagcg tgacgttggc
     8941 ggatatgcgg tggcgctggc gacctcgatg gacaccattt ttggcggcaa agcgggcggt
     9001 gtgttctttt gtgcttcgga tatcggctgg gtggtggggc attcgtatat cgtctacgcg
     9061 ccgctgctgg cggggatggc gactatcgtt tacgaaggat taccgacctg gccggactgc
     9121 ggcgtgtggt ggaaaatcgt cgagaaatat caggttagcc ggatgttctc agcgccgacc
     9181 gccattcgcg tgctgaaaaa attccctacc gctgaaattc gcaaacacga tctctcgtcg
     9241 ctggaagtgc tctatctggc tggagaaccg ctggacgagc cgaccgccag ttgggtgagc
     9301 aatacgctgg atgtgccggt catcgacaac tactggcaga ccgaatccgg ctggccgatt
     9361 atggcgattg ctcgcggtct ggacgacagg ccgacgcgtc tgggaagccc cggcgtgccg
     9421 atgtatggct ataacgtgca gttgcttaat gaagtcaccg gcgaaccgtg tggcgtcaac
     9481 gagaaaggga tgctggtggt ggaagggccg ctgccgccgg ggtgtattca gaccatctgg
     9541 ggcgacgacg accgctttgt gaagacgtac tggtcgctgt tttcccgccc ggtgtacgcc
     9601 acctttgact ggggcatccg tgacgctgac ggttatcact ttattctcgg gcgcactgac
     9661 gatgtgatta acgttgccgg gcatcggctg gggacgcgcg agattgaaga gagtatctcc
     9721 agtcatccgg gcgttgccga agtggcggtg gttggggtga aagatgcgct gaaagggcag
     9781 gtggcggtgg cgtttgtcat tccgaaagag agcgacagtc tggaagatcg tgatgtggcg
     9841 cattcgcaag agaaggcgat tatggcgctg gtggacagcc agattggcaa ctttggccgc
     9901 ccggcgcacg tctggtttgt ctcgcaattg ccaaaaacgc gatccggaaa aatgctgcgc
     9961 cgcacgatcc aggcgatttg cgaaggacgc gatcctgggg atctgacgac cattgatgat
    10021 cctgcgtcgt tgaatcagat ccgccaggcg atggaagagt aaggtattgt cggatgcgtc
    10081 gcgtggcgca tccgacagtt gtgcgcagat gcctgatgcg acgc
//
LOCUS       DAZFKY010000170        10081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFKY010000170 DAZFKY010000000
VERSION     DAZFKY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            125..340
                     /gene="bdm"
                     /locus_tag="ACHKCL_003808"
     CDS             125..340
                     /gene="bdm"
                     /locus_tag="ACHKCL_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5764691.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            442..579
                     /gene="sra"
                     /locus_tag="ACHKCL_003809"
     CDS             442..579
                     /gene="sra"
                     /locus_tag="ACHKCL_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5764692.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            736..2433
                     /gene="maeA"
                     /locus_tag="ACHKCL_003810"
     CDS             736..2433
                     /gene="maeA"
                     /locus_tag="ACHKCL_003810"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5764693.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGISGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2567..3577
                     /gene="adhP"
                     /locus_tag="ACHKCL_003811"
     CDS             2567..3577
                     /gene="adhP"
                     /locus_tag="ACHKCL_003811"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5764694.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3723..4007
                     /locus_tag="ACHKCL_003812"
     CDS             3723..4007
                     /locus_tag="ACHKCL_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5764695.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(4235..4888)
                     /gene="fdnI"
                     /locus_tag="ACHKCL_003813"
     CDS             complement(4235..4888)
                     /gene="fdnI"
                     /locus_tag="ACHKCL_003813"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5764696.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4881..5765)
                     /gene="fdnH"
                     /locus_tag="ACHKCL_003814"
     CDS             complement(4881..5765)
                     /gene="fdnH"
                     /locus_tag="ACHKCL_003814"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5764697.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5778..8825)
                     /gene="fdnG"
                     /locus_tag="ACHKCL_003815"
     CDS             complement(5778..8825)
                     /gene="fdnG"
                     /locus_tag="ACHKCL_003815"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8238..8240),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5764698.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9057..9938
                     /gene="yddG"
                     /locus_tag="ACHKCL_003816"
     CDS             9057..9938
                     /gene="yddG"
                     /locus_tag="ACHKCL_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5764699.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2453 a         2650 c         2535 g         2443 t
ORIGIN      
        1 tataaaaatc atcaaccagg actaatctta acaacgaagc ggcaaatatt tgccatgctg
       61 aatgtgcttt ataaatctgc gatccgtagc agacactata aatacacaga cacggagaat
      121 cactatgttt acttattatc aggcagaaaa ttcaacagca gaacccgctc tggttaatgc
      181 cattgagcaa ggccttcggg cagagcacgg tgttgtcact gaagatgaca ttctcatgga
      241 gctgaccaaa tgggtggaag cttctgataa cgacatcctc agtgatatct accagcaaac
      301 gatcaattat gtggtcagtg gccagcaccc tacgctttaa gatgctatgc ttgatcggca
      361 acctaattta ggggtttagc acgtgtttct tcgctacggc gatgttgtcc ttaaaactag
      421 caacaggatt gaggagttaa aatgaaatcg aaccgtcagg cacgtcatat tcttggactg
      481 gaccataaaa tatctaacca gcgcaaaata gttaccgaag gtgacaaatc cagcgtggta
      541 aataacccaa ccggcagaaa acgccccgct gaaaagtaat tcataaccat cagtcctcaa
      601 tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt ttttatctgc tttatacttg
      661 gggccgacgc cctggcggta aagcaaagac gataaaagcc ccccagggat ggatattcaa
      721 aaaagagtga gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac
      781 gctggccctg tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa
      841 gaacgccgta acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa
      901 caagcggaac gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc
      961 tacctgcgta acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat
     1021 cttgatgaga tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt
     1081 tctgagatct accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg
     1141 gacgatattc tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt
     1201 gaacgtattc tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa
     1261 ctgtcgctct ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg
     1321 gatgtcggaa cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat
     1381 ccgcgtatca ctgacgacga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa
     1441 caacgctggc cagacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg
     1501 ttacttaacc gctatcgcaa tgaaatttgt tcctttaacg atgacattca gggcaccgcg
     1561 gcggtaacag tcggcaccct gatcgcagca agccgcgcgg caggtggtca gttaagcgag
     1621 aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc
     1681 gcccagaccc agcgcgaagg attaagcgag gaagcggcgc ggcagaaagt ctttatggtc
     1741 gatcgctttg gcctgctgac cgacaagatg ccgaacctgc tgcctttcca gaccaaactg
     1801 gtgcagaagc gcgaaaacct cagtgacagg gataccgaca gcgatgtgct gtcactgctg
     1861 gatgtggtgc gcaatgtaaa accagatatt ctgatcggca tctcaggaca gaccgggctg
     1921 tttacggaag agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg
     1981 ctgtccaacc cgacgtcacg cgtggaagcc acaccgcagg acattatcgc ctggactgaa
     2041 ggtaacgcgc tggtcgccac gggcagtccg tttaatccag tggtatggaa agataaaatc
     2101 taccctatcg cccagtgtaa caacgccttt attttcccgg gcatcgggct gggtgttatt
     2161 gcttccggcg cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgcc
     2221 cagtattcgc cgctggtcct gaacggcgaa ggtctggtac taccggaact aaaagatatt
     2281 cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg
     2341 gcggtgaaaa cgtctgccga agctttgcaa caagccattg acgataattt ctggcaagcc
     2401 gaataccgcg actaccgccg tacctccatc taagcctgcg cccggtagtg aaggctaccg
     2461 ggctatttcc ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc
     2521 atttgcctca cctgctatgc agaacatcat ccgaaaagga ggaactatga aggctgcagt
     2581 tgttacgaag gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg
     2641 cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa
     2701 tggcgatttt ggtgacaaaa ccggcgtaat tctgggccat gaagggattg gtgtggtggc
     2761 agaagtgggt ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt
     2821 ctacgaagga tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc
     2881 agttaaaaat gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc
     2941 cgattacgcg gtaaaagtgc cagatggtct ggactcggcg gcggccagca gcattacctg
     3001 tgcgggggtc accacctaca aagccgttaa gctgtcaaaa attcgtcccg ggcagtggat
     3061 tgctatctac ggtcttggcg gtctgggtaa cctcgccctg caatacgcga agaatgtctt
     3121 taacgcgaaa gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat
     3181 gggtgcagat ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa
     3241 aaccggtggc gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc
     3301 agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat
     3361 gagcctggat atcccacgtc ttgtgctgga tggcattgag gtggtcggtt cgctggtcgg
     3421 cacgcgccag gatctaactg aagccttcca gtttgccgcc gaaggtaaag tggtgccgaa
     3481 agtcgccctg cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa
     3541 aatccgtggc cgtatggtga ttgatttccg ccgctaagag gcctttgctg cgactcccat
     3601 gttcgggtcg cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg
     3661 taggaaggaa caataatgat agtgttccca gagaaggaac gctactgaga gtaaagacac
     3721 taatgaaaat ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac
     3781 ttaatgtcag cctgcgcgag tttgccagag caatggaaat tgctccctca acggcaagcc
     3841 gattgctgac cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga
     3901 tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggctg
     3961 caaaaacggt ggatgtgtcg cgattacgcc gtctggttgc gcaataaaaa atggcgcctt
     4021 ttacagcgcc atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca
     4081 ggcctacacg ttcactgcaa tctattggat ttgaacggtt tgtaggccgg ataaggcgtt
     4141 cacgccgcat ccggcatgaa caaagcctac gttgtctaca atatgaaaat ggcgcctttt
     4201 acagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt
     4261 tcgcttctgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac
     4321 ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat gccatataca
     4381 tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt
     4441 agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg
     4501 tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt
     4561 tgtacttacc gacatccgcc actttatgct cattgccttt caatacttcg acaatgttta
     4621 acagccacgg aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa
     4681 acatcagtgc gacgaaaatc gcaatgccga agaatgggtg caaaatgcgt cccatctgcg
     4741 gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca
     4801 gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg
     4861 tgcgcacaat cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta
     4921 ttcgggccaa taccgatgta gtggaaaatc aaccctgcaa aagtggcaat aaagccagcc
     4981 gctgccagcg gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct
     5041 ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc
     5101 gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt
     5161 ttcgccacgc gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc
     5221 ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag
     5281 agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac
     5341 ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta
     5401 gcgtactgaa tgattgcacc ggcagacggg cacgccttca ggcagcccgg atcttcacag
     5461 tgcatacagc catctttacg gatcagccac tccagcttgc cgctctgttc ggtttcgcta
     5521 aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta aaccccgacg
     5581 cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta
     5641 cagccgatac aggtggaaac gtcgataagt tttgcgactt ctgctttgta atcacgcacc
     5701 tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg cgtttccata
     5761 gccatctgtt cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc
     5821 gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagctatata acctttacgc
     5881 gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccattt
     5941 acattcagcg gtttcagacg acgcgttacc acagccaccg cgcggataaa gccacgcttg
     6001 cttgagacag tgacacgatc gccattatta atgcctttcg ccgccgccag cgtttcgctg
     6061 atttccacaa actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg
     6121 tggaagtgct cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc
     6181 atccgcagcg cgtcttgttc atacagacga accaccgggt tagaaaccac gttcggatgc
     6241 agcgggttag ttcccagcgg cgtttcaatt ggctcgtagt gttccgggaa cggaccttcc
     6301 gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat aaacggcccg
     6361 gttggcgtac ccggtgcggc attgccgaag tcagggatat cgttacccgt ccacttgctg
     6421 ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat atctgccgaa
     6481 gcacggttgt agagtacgcg acggttaagc ggccacgccc aggcccatcc cagcgtattc
     6541 cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg ctctgtccag
     6601 ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag
     6661 ctactcagca actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc
     6721 agcgcatagc cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg
     6781 tagttccagc tcatcttcat cagcggttct acgcctttac caccttcagc ctggtacagc
     6841 tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc
     6901 gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatct
     6961 tcttctgcaa agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg
     7021 acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg
     7081 ataaccacca tgtacttcag cttgctcagg cagctcacca ctttgttttt gtccgggaag
     7141 gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttgccttc atccatcatg
     7201 ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag
     7261 ttgttctctt tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc
     7321 ggatagttgc tccagtagtt cacctgacca gccagcgtcg ctttcggcgt gttcgcttcc
     7381 agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg
     7441 gtagagagca gacctaagtc agtcagaccc tgaatgttgg agtgaccacg caatgcattc
     7501 acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg
     7561 atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg
     7621 cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc
     7681 gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgctc tttcagcagg
     7741 ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt ttgcatagcc gttttcatcg
     7801 aactgatagt tccaggacga cttatcgtac tggcgttttt cagcgtcgta gccgctgaac
     7861 agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcgttggt gtaatgctta
     7921 acgtattcgg cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac
     7981 gtaatgtccg taccggaacg aataggcgcg tagatatccg ccacagaagc ggtacgcgta
     8041 aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag
     8101 cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg
     8161 tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg agcaagactt
     8221 gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag
     8281 cgggcaaatt tctgggtcag catcccggtt tcgttgctgg caccggaagc acacagcata
     8341 ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg
     8401 tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc
     8461 tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcgctgttg
     8521 acgtaatcca gcaaaccggc ccctttcggg cacagcgccc cacggcttac cggatgatcc
     8581 gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc acccaggcta
     8641 tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg
     8701 cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc
     8761 gctactgttg ttccagccat accgcccgcg cagattttaa aaaattgtct gcgactgacg
     8821 tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc
     8881 tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg
     8941 caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa
     9001 aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga
     9061 cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag
     9121 gattgattcg cggtgtcagt gaggggctcg gcccggtcgg tggcgcagct gctatctatt
     9181 cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg caaatcccga
     9241 aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt
     9301 ccttagggta tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt
     9361 ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga
     9421 ttgtaccagg attattatta gccctcgtcg gcgtctgctg ggtgttaggc ggtgacaatg
     9481 ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg
     9541 cgttcattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa tacgcacgcg
     9601 gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg gtttactatt
     9661 ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa ctcatctctg
     9721 cggcatttac cttaggattt gcttatgctg catggaatgt cggtatactg catggcaacg
     9781 tcaccataat ggcggtaggt tcgtatttta cgcccgtact ttcctcagcg cttgcagccg
     9841 tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctgatg gtctgcggcg
     9901 gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgccgg gtctacggcc
     9961 cggcaattaa tcagataaac cccgacaata ttaaaaaata aatttcaaat accagtaatt
    10021 caccttatat ctcatttcga ctccgatcac atatcgataa ataatattat ttcatctatt
    10081 t
//
LOCUS       DAZFKY010000171        10046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFKY010000171 DAZFKY010000000
VERSION     DAZFKY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..81
                     /locus_tag="ACHKCL_003817"
     tRNA            6..81
                     /locus_tag="ACHKCL_003817"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKCL_003818"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKCL_003818"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHKCL_003819"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHKCL_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5764700.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKCL_003820"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKCL_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5764701.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWVVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHKCL_003821"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHKCL_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5764702.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHKCL_003822"
     CDS             complement(3595..4620)
                     /locus_tag="ACHKCL_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5764703.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHKCL_003823"
     CDS             complement(5051..5272)
                     /locus_tag="ACHKCL_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764704.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHKCL_003824"
                     /pseudo
     CDS             complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHKCL_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHKCL_003825"
     CDS             complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHKCL_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5764705.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2668 a         2591 c         2428 g         2359 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactacc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga gctatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctggaaaaca taaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagccagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccattag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcctacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg ctaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggtgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattgg
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag ccttatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ctccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc tgtagattca attggtcaac
     6781 gcaacagtta tgtgaaaaca tggggttgcg gaggtgcctg gccgctgaag ctaaagccgt
     6841 taaccgtaaa gacactttca acgtttgctt tctcgttctt cagatagtaa tccgtcactt
     6901 gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga atcatggtca
     6961 gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa aacaacacca
     7021 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
     7081 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
     7141 agaaaccacc cttattttcg tgatgctcag ctgaggtggg tttgagcagt gttgcacata
     7201 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
     7261 tagagaactg acgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
     7321 ccgctgacag caccatcgcg atacccacca gtgcgccctg tatttgcgac attgattttt
     7381 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
     7441 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
     7501 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
     7561 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     7621 acaccagcat aatagcttcg aacagtgttt ttaccacttc gtgaatagaa agctggacga
     7681 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     7741 ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg gttgccagct
     7801 taatacccag gcccgccgcc ggttttccgt tgatgcgagc gataacgtta tagttttcac
     7861 cgccaagttc aacccgtgcg acatctttca gacgcaccac cgagccgtca ctgtttacgc
     7921 gcagggtcac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     7981 cattcaactg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca gcgatctgag
     8041 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     8101 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     8161 cgctattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     8221 cctgtgtggt gtctgggtta tcagagacaa agcctgccac catcaaatag ctgctactgg
     8281 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     8341 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     8401 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     8461 taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     8521 cgcccggata gtttgctgaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     8581 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     8641 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     8701 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     8761 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     8821 cgcctgactg gcaacgacag ggcgcacttc cacctgactt ttatcgttaa caatcagcac
     8881 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggatgagaa tggcgtcagg
     8941 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     9001 gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg tcacatcgga
     9061 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     9121 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gactgcttca gcctcataaa
     9181 atcgttgctg gattgggtca catcaacata gataggatcg agctgctgga cagttgccag
     9241 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt tgccgatacg
     9301 tccgctaatt ggcgcagtca ctttggtata agcaagattg atgcgagcgc tttcgactgt
     9361 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     9421 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     9481 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     9541 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat cgctgccttc
     9601 agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacgata
     9661 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt ttacaatatg
     9721 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccggc
     9781 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     9841 cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca acgcgttaac
     9901 tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga tgtaaggaat
     9961 agttatgaat acaggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
    10021 tttattttga cgcgggttga aagagg
//
LOCUS       DAZFKY010000172         9911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFKY010000172 DAZFKY010000000
VERSION     DAZFKY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..243
                     /locus_tag="ACHKCL_003826"
     CDS             <1..243
                     /locus_tag="ACHKCL_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5764706.1"
                     /translation="LAVPPAYTSQRCACCGHTAKENRLSQSKFRCQACGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            287..394
                     /locus_tag="ACHKCL_003827"
                     /pseudo
     CDS             287..394
                     /locus_tag="ACHKCL_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(378..1016)
                     /gene="ytfB"
                     /locus_tag="ACHKCL_003828"
     CDS             complement(378..1016)
                     /gene="ytfB"
                     /locus_tag="ACHKCL_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5764707.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1234..1854
                     /gene="fklB"
                     /locus_tag="ACHKCL_003829"
     CDS             1234..1854
                     /gene="fklB"
                     /locus_tag="ACHKCL_003829"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5764708.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2163..3575
                     /gene="cycA"
                     /locus_tag="ACHKCL_003830"
                     /gene_synonym="dagA"
     CDS             2163..3575
                     /gene="cycA"
                     /locus_tag="ACHKCL_003830"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5764709.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3620..4282)
                     /gene="ytfE"
                     /locus_tag="ACHKCL_003831"
     CDS             complement(3620..4282)
                     /gene="ytfE"
                     /locus_tag="ACHKCL_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5764710.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4390..5355)
                     /gene="ytfF"
                     /locus_tag="ACHKCL_003832"
     CDS             complement(4390..5355)
                     /gene="ytfF"
                     /locus_tag="ACHKCL_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5764711.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5463..6323)
                     /gene="qorB"
                     /locus_tag="ACHKCL_003833"
     CDS             complement(5463..6323)
                     /gene="qorB"
                     /locus_tag="ACHKCL_003833"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5764712.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            6412..6792
                     /gene="ytfH"
                     /locus_tag="ACHKCL_003834"
     CDS             6412..6792
                     /gene="ytfH"
                     /locus_tag="ACHKCL_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5764713.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6910..8853)
                     /gene="cpdB"
                     /locus_tag="ACHKCL_003835"
     CDS             complement(6910..8853)
                     /gene="cpdB"
                     /locus_tag="ACHKCL_003835"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5764714.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9043..9783
                     /gene="cysQ"
                     /locus_tag="ACHKCL_003836"
     CDS             9043..9783
                     /gene="cysQ"
                     /locus_tag="ACHKCL_003836"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5764715.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2268 a         2514 c         2607 g         2522 t
ORIGIN      
        1 cttgctgttc cgccagcgta cacaagccag cgttgcgcgt gctgtggtca tacagcgaaa
       61 gaaaatcgcc tgtcacaaag taaattcaga tgccaggcat gtggatatac agcgaacgct
      121 gatgtaaatg gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggggag
      181 atggtgcagt caggccgccc gttgaagcag gaacccaccg aaatgattca ggcgacagcc
      241 tgaacgtagc agggatccac gtccttcagg gcttggagga tgtcaacaca tcagtatggt
      301 ttcaccccag gcactggaag ccagacgtaa ggtcatcaaa gagacaatcc ttagctggct
      361 tttggttgac ccttcatcta ccgcgcacga ataaaactgc catccggctg gcgggtgaac
      421 aggacctgtt gattattccc cgtatcaatg gttaagcccg tcaccacgcc actggcgttc
      481 tggcggattt tcaccatctg cccgttttgc aaattactca gcggcttacc cgcgccttct
      541 acctgcgcca ttgcatagac atcggtagcg ggcagtccgt ggtcgcggaa cagctgcgcc
      601 atcgttttac cgggttcgac acggtaagag cgccattgat tatcaattcc gctatctggc
      661 tggaatggtt gcgtttgggt agttttcggc tgttcttctg gctggccttc ctgtataggt
      721 tctggagcga ccggagccac ctgatcagga taattttgtg gggtgactaa ttgcgcccgc
      781 agctgttctt ctgtcggcgg ttgtgattgc gactgaatat ctaactgcgc ttcacgcgtc
      841 accaccggcg cgttgggcgt atcatcagat ggaagcagaa atccgaccac caatacaatg
      901 gcggcaataa tgatcccccg acgatgcatc ggcggcagcg ggtccatgaa gcgaaaattg
      961 tccggtgcgt gccagacttt ctccagggtt ggttttagtt caaagcgccc gggcatggtt
     1021 tcctcctgct ccgcgtcttg ttcctcaatc atagcctatg aataagctaa cgctatgatg
     1081 tccgtggtaa acccgccttt attatattca tacgggatat tgctattgtt tctttttccc
     1141 tgggatttgt catcattccc gcgacaaagt tttacccaaa gaagtgtggc tgatatgctg
     1201 cccgctactt tataccctaa gaaaggaaat acgatgacca ccccaacttt tgacaccatc
     1261 gaagcgcaag caagctacgg cattggtttg caggtagggc aacaactgag tgaatctggc
     1321 ctggaagggc tgctgccaga agcactggtt gcaggtattg ccgatgcgct ggaaggcaaa
     1381 catccggctg ttccggttga tgtggtgcat cgcgcgctgc gtgaaatcca cgagcgcgcc
     1441 gatgccgttc gtcgtcagcg tttccaggcg atggctgctg aaggtgtgaa atacctggaa
     1501 gaaaacgcca aaaaagaagg tgtgaatagc accgaatctg gcctgcaatt ccgcgtgatc
     1561 aaccagggtg aaggcgcaat tccggcacgt accgaccgcg ttcgtgttca ttacaccggt
     1621 aaactgatcg acggcaccgt gtttgacagc tccgttgctc gtggtgaacc cgctgaattt
     1681 ccggttaatg gcgtgatccc tggctggatt gaagcactga ctctgatgcc ggtaggttct
     1741 aaatgggaac tgactatccc gcaggaactg gcatatggcg agcgcggcgc aggcgcatcc
     1801 atccctccgt tcagcaccct ggtgtttgaa gtcgaactgc tggaaatcct ctaagcagcg
     1861 cattctgttc ccctcgaacc agaggggagc aggcattcag caataaaccc ttcagtttgc
     1921 caaacggcgc tattttgtgt tgcaaagacc ccgtaagcgt gtatttttgt gagctgtttc
     1981 gcgttatcac cgtgatatga cactcacttt aaacataaaa ttaacattag atctaaatct
     2041 tagtattcat cccgcgtatt gttacctaat atcgatgagt cccgatacag attcgtcgta
     2101 tcatagactg actaaaggcc gtagagcctg aacaacacag acaggtacag gaagaaaaaa
     2161 acatggtaga tcaggtaaaa gtcgttgccg atgatcaggc tccggctgaa cagtcgctac
     2221 ggcgcaatct cacaaaccga catattcagc ttattgccat tggtggtgcc attggtacgg
     2281 ggttgtttat ggggtctggc aaaacgatta gccttgccgg gccgtcgatc attttcgttt
     2341 atatgatcat tggttttatg ctctttttcg tgatgcgggc aatgggggaa ttgctgcttt
     2401 cgaatctgga atacaaatct tttagtgact tcgcttccga tttactcggg ccgtgggcag
     2461 gatatttcac cggctggact tactggttct gctgggttgt aaccggtatg gcagacgtgg
     2521 tggcgatcac ggcttatgct cagttctggt tccccgatct ctccgactgg gtcgcctcgc
     2581 tggcggtgat agtgctgctg ctgacgctta acctcgccac tgtgaaaatg ttcggtgaga
     2641 tggagttctg gtttgcgatg atcaaaatcg tcgccattgt gtcgctgatt gtcgtcggcc
     2701 tggtcatggt ggcgatgcac tttcagtcac cgactggtgt ggaagcgtca ttcgcgcatt
     2761 tgtggaatga cggcggctgg ttcccgaaag gtttaagtgg cttctttgcc ggattccaga
     2821 tagcggtttt cgctttcgtg gggattgagc tggtaggtac aacagctgcg gaaaccaaag
     2881 atccagagaa atcactgcca cgcgcgatta actccattcc gatccgtatc attatgttct
     2941 acgtcttcgc gctgattgtt attatgtccg tgacgccgtg gagttcggta gtcccggaga
     3001 aaagcccgtt tgttgaactg tttgtgttgg taggtttgcc tgcggctgcc agcgtgatca
     3061 actttgtggt gctgacctct gcggcgtctt ccgctaacag tggcgtcttc tctaccagcc
     3121 gtatgctgtt tggtctggcg caggaaggtg tggcaccgaa agcgttcgct aaactttcta
     3181 agcgcgcagt acccgcgaaa gggttgactt tctcgtgtat ctgtctgctc ggcggcgtgg
     3241 tgatgttgta tgtgaatcct agcgtgattg gcgcgttcac gatgattaca accgtttccg
     3301 cgattctgtt tatgttcgtc tggacgatta tcctttgctc gtaccttgtg tatcgcaaac
     3361 agcgtcctca tctacatgag aagtcgatct acaagatgcc gctcggcaag ctgatgtgct
     3421 gggtatgtat ggcgttcttt gtgttcgtgg tcgtgttgct gacactggaa gatgacactc
     3481 gccaggcgct gctggttacc ccgctgtggt ttatcgcgct ggggctgggc tggttgttta
     3541 ttggtaagaa gcgggctgct gaactgcgga aataaccgca ttatcatgct ggatggcgca
     3601 atgccatcca gcttttagat cactcacccg ccagcgccct tgggaacagt acattgtttt
     3661 ccaggctgat gtgatccatc aggtcatcaa tcagttcatt aatgccgtta tacatcgctt
     3721 tccaggtggt gcaggcttcc ggcggcggtg tgacgttatt ggtattgtgt ttgatcactt
     3781 ccagcagttc acccgcttca tcgtgctcgc tttccattac gctgattggc cccattgcct
     3841 ggctgcccat gccttgtttg atcatcggga agaggatctg ctcttctttc atcatgtggc
     3901 tggaaagctc ttcatgcagc atggtcaggt attttgtcag cccttttggc acgctcggtt
     3961 tgtcggcgtg aacgcgctcg actttagtcg cttgcagaat cagctccggc agttgctcgc
     4021 ggtgacgagc gtggtagcgc acgatgatat ggtcgatgat ttctgccagc ggggcgctac
     4081 gccagtcttt ctcaatcggt tgttcagcaa gctttgccag ctcagcttca atgacctcaa
     4141 catccagttc tttacgtgcc gccgcacgcg ccagcgtttg cttaccgccg cagcagtaat
     4201 ccatatcata tttacgaaac agagccgaag cgcgaggaat agagagcgcc agttcaccta
     4261 aaggttggtc gcgataagcc atagctgata cctcattctt aataagataa gatgtatttt
     4321 aaatgcatct ttaaggcaaa agctataacc cttacgtagc agaggcttat ttaactcact
     4381 gccagcgtgt caactttctg agacagattc agttaaaggt ttttctggtt ttgctctaac
     4441 cgcgatgacc acgccaatca ctaacagcgc gataccgctc agcgtcatta gcggcggcat
     4501 ttgctggcgg agtaaaaagg tgtacagcaa acctgccagc gtttcgaaaa caatcagcgg
     4561 cccgagaatc actgtcggta atcgctggct ggcgacgttc cagcagagtg cgccaaccca
     4621 tgagcaaagc acggctatcg caatcatcag actaataaac accagcggac gggggccaaa
     4681 gggtagggag aagtccggcg tttgcgtatt cagccagtaa caagcgacga gatagccgat
     4741 aagtgaaacc ggcagtgtga ccagcgcctg cgctgttgcc cacatcatcg gatgtttgtc
     4801 gggattttcc cgcagccagc gggcgttgcg cagggcatac cacgcccagc agaccacgga
     4861 aactaacgcc agcacgatgc cagaggtata acgtgcccag tcaaaattgg ggagtccgtg
     4921 gtttaactca gcaatattca cacacgccag gccgatgcca atacaaatca gtgccggggc
     4981 gagttttccc cacgcgagtt tgccgtcgcg ctggctataa agcagattgg cgaagacggg
     5041 aatcaccacc ggcagggtgc cgataatcat cgtggaaaca ggcgcaccag tacgttgaat
     5101 ggcactggca aggcagaagt aatagatgag gttgcccatc atagtgagca tcaaggcggt
     5161 aagccagtcg cgacgcgcca actgacgcag acgcacgcgt cccagccagg caatgggcag
     5221 cgcaattaac cctaacgcca gataacgccc catcgactgc aacatcgccg ggtattccgg
     5281 cacgatcaac ggcccgacaa aaataagccc ccacatcaac cctgctaaca gggcgtacag
     5341 cacgccgcta atcattactg gcatccattg atctgtcaga agaaagttca ggaagcagac
     5401 attattgctc ccggcatccc gtcattatca gagaggatgc gggaggatgc cactttaatt
     5461 aactagttat taacattaaa aagatggctt acgctttcgg ctaacgttgt cgttgggtgg
     5521 ccaatcaatt tgctaagtgt tttgctgtca tcaaacagac cgcctttcga tgcgccaacg
     5581 tcagaatccg ccagcatatc cgccagtccg tcgggcagtc cgacgctttt cagtgccgcg
     5641 gcgaaatcgg cttcgctcag attttgatag gtaacctgtt tgccgctctg tttggtcagc
     5701 tctgccgcta actgtgtcaa cgtccaggca ctatcgcccg ccagttcgta aaccttgcct
     5761 tcgtgaccgg cttcgctaat cacgcgtgcc gcagctgccg cataatctgc ccgcgttgct
     5821 gaggcgattt tgccatcgcc cgccgcaccg ataaatacgc cgtgttccag tgctgccggg
     5881 gcgctggcga ggtagttttc ggtgtaccag ccgttgcgca gtagggtgta aacgatgcca
     5941 gaatcagcca gcattttctc cgtctcgatg tgctcatcgg cgaggccgag cggagattta
     6001 tccgcgtgaa gcaggctggt ataagcgata aatttcacgc cagccgtctt tgcggcatta
     6061 ataacattac gatgctgcgg ggcacgttga cccacttcgc tggaagagat cagcagtagt
     6121 ttttccactc cctgaagtgc agatgtcagt gcggcttcat cgccgtagtc agcctgacgc
     6181 acggtaatgc cttgtgctgt cagtgcctgg gcttttgccg gattacgaac gatagccact
     6241 atttggctgg caggaaccgt tttcatcaag gattcaataa catactgacc aagttggcca
     6301 gtggcaccag taatagcgat catgggaagt ctccatcgtt ttgtcttgtg tttaaatacg
     6361 ctaacaccta aacttacttt tagtaagtac gcacaaaaag gtaagtatga aatgagtcag
     6421 gttagcctgt cgcaacaact gaaagagggt aatctctttg cggaacagtg cccgtcgcgc
     6481 gaggtgttga aacacgtcac cagccgttgg ggggtgttga ttctggtggc gctacgcgaa
     6541 ggtactcatc gctttagcga cctgcggcgc aaaatcggcg gggtgagcga aaagatgctt
     6601 gcgcagtcgt tacaggcgtt agaacaggat ggttttctta accgtatcgc gtatccggtg
     6661 gtgccgccgc atgttgaata cagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt
     6721 gctgcactgg cggactggat tgagttgaat ttgcccgagg tgttggcggt gcgggatgaa
     6781 cgtgcggcat aactgccgga tgcgcggcct acggtgtatg acttgtaggg cggataagat
     6841 gcgccagcat cgcatccgcc aataaatgcc ggatgcgatt gatgatttat aggccgaaaa
     6901 agaagtgttt tacttactca aatccacctg ataaatcgca aacccgatat catcggtcgc
     6961 gactttattc atcggatact gccctttctc tttaataaac gctgcggctt tatctgacgg
     7021 ggaagtttcg aaacggatat ccagtttctt atcggcagct atcggtgcta aacgccagtt
     7081 gttatctgcc gccgggtgaa tttcccccgc acgtttcgac tcatcagcaa tccacgctgc
     7141 cagcaccgag cggttctcat ccggtgaagc aaaagcgata tggctgtcgc ccgtcccggc
     7201 aaatttgccg ccgtaagcgc gatagttatt ggtggcaacg aggaacatag cgttcggatc
     7261 aatcggcttg ccattaaagg tcaggttctt aatcctttcc gcattggcat taatcatctg
     7321 gcactcgccg tcataacggg cgggctgggt aacatcaatc tgataattca caccatcaat
     7381 aacatcaaag ttataagtgc ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct
     7441 gttgggatca atctggttaa actgtccggc ggagcactcc agccactctt tcacctcttt
     7501 accgctggct ttcaccacaa tcagcgtatt gggatagaga taaagatcgg cggcattacg
     7561 gaaggtcaac tggccttttt ccacctccac atagcttgcc gggtcatttt tgcgaccacc
     7621 gactttaaac ggtgcggcag ctgaaagcac cggcagtttt gccagatccg gatcgccctg
     7681 aatgtaatgc tcgacatacg ctttttgcgc gttgttcact acttgcacgg tcggatcgtc
     7741 ctgcaccagc gccagatagc tatacatatt gtcggcagat ttaccgattg gcttgctgac
     7801 gaactggcgt gtggcatcgt gatcggcttt gagtgtttct accagcttgc tgtcttccgc
     7861 cgcgagggat tttttattgg cgatgtcgta aatcggccgt gcttccgctt tcgcctgcgt
     7921 cacctgccat ttaccgctgt cattactgag ttgtaagtcg accaccccaa gatgatcgcc
     7981 ccacatgccc ggcattaccg ccggaacacc attcagcgtg cctttggtga tatcagcccc
     8041 ttcgatatca gcaaaatctt tacccgggaa aacggcgtga gcatggccaa acataatggc
     8101 gttaacgccc ggaatttcac tgaggtaata aactgagttt tccgccatta ctttatacgg
     8161 atcggcagac agcccggaat gcgccagaac gacaacgaca tcggcacctt tctcgcgcat
     8221 ttcaggctcg tatttgcgca cggtttcggt aatatcattc accgtcactt ttccggataa
     8281 attagcttta tcccagccca tgatttgcgg cggcacgacg ccaatatagc caatcttcag
     8341 cgtctgtttt tttccgtctt tatcgaccac ttcggtgtct ttaattaaat acggtgtaaa
     8401 cattggctgt ttggttctgg cgtcaatgac gttggcattt acataaggga atttcgctcc
     8461 tgccagcgca tttttcaggt aatccagacc gtagttaaac tcgtggttgc caagcgttcc
     8521 gacggtatag tccagcgtat ttaatgcctt atagaccggg tgaatatcac ctgcttttaa
     8581 tcctttcgcc gacatgtaat cggccagcgg actcccctga atcaaatcgc cgttatcgac
     8641 cagtacgctg tttttcactt cattgcgggc attgttaatc aggcttgccg tacgtaccag
     8701 tccgaatttt tccgtggcgg tgtctttgta ataatcgaaa tccatcatgt tgctatgcag
     8761 atcagtggtt tccatgatac gcagatcgac cgtcgctgca ttcacactgg cggcaatcag
     8821 cgtggccagg agcgttgcgc taaacttaat catcagggac atccttttat catcgggaat
     8881 acgaaagaaa agggagaata aacgtcttac ttatagaaca gtgaagaatg ccacaatttt
     8941 acgctttgaa aatgatgaca ctatcacagt tggcgcattc attaatgata gggtataagt
     9001 aaaacaataa gttaacaccg ctcacagaga cgaggtggag aaatgttaga tcaagtatgc
     9061 cagcttgcac ggaatgcagg cgatgccatt atgcaggtct acgacggggc gaaaccgatg
     9121 gacgtcgtca gcaaagcgga caattctccg gtaacggcag cggatattgc cgctcacacc
     9181 gttatcatgg acggtttacg tacgctgaca ccggatattc cggtcctttc tgaagaagat
     9241 cctcccggtt gggaagtccg tcagcactgg cagcgttact ggctggtaga cccgctggat
     9301 ggtacgaaag agtttattaa acgtaatggc gaattcaccg ttaacattgc gctcattgac
     9361 catggcaaac cgattttagg tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg
     9421 gcagaaggca aggcctggaa agaagagtgc ggcgtgcgca agcagattca ggtccgcgat
     9481 gcgcgcccgc cgctggtggt gatcagccgc tctcatgcgg atgcggagct gaaagagtat
     9541 ctgcaacagc ttggcgaaca tcagaccacg tccatcggct cttcgctgaa gttctgcctg
     9601 gtggcggaag gacaggcgca gctgtacccg cgtttcggac caacgaatat ttgggacacc
     9661 gccgctggac atgctgtggc tgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa
     9721 ccgctggatt acactccgcg cgagtcgttc ctgaatccgg ggttcagagt gtctatttac
     9781 taaattcaga tggcagaaac agtgtatttc ctgattctgc catcctgatt tctcccaacc
     9841 taaaaagtta taaataaaaa gagattgtat ttaaagtgca aaaattcaat tgctaataag
     9901 ttacatttta a
//
LOCUS       DAZFKY010000173         9697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFKY010000173 DAZFKY010000000
VERSION     DAZFKY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..773
                     /locus_tag="ACHKCL_003837"
     CDS             <1..773
                     /locus_tag="ACHKCL_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5764716.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ACHKCL_003838"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ACHKCL_003838"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO5764717.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHKCL_003839"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHKCL_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5764718.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHKCL_003840"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHKCL_003840"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5764719.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHKCL_003841"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHKCL_003841"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5764720.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHKCL_003842"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHKCL_003842"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5764721.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ACHKCL_003843"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ACHKCL_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5764722.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHKCL_003844"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHKCL_003844"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5764723.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2210 a         2402 c         2682 g         2403 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttcttta
//
LOCUS       DAZFKY010000174         9697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFKY010000174 DAZFKY010000000
VERSION     DAZFKY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            28..102
                     /gene="tnaC"
                     /locus_tag="ACHKCL_003845"
     CDS             28..102
                     /gene="tnaC"
                     /locus_tag="ACHKCL_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5764724.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            323..1738
                     /gene="tnaA"
                     /locus_tag="ACHKCL_003846"
     CDS             323..1738
                     /gene="tnaA"
                     /locus_tag="ACHKCL_003846"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5764725.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1829..3076
                     /gene="tnaB"
                     /locus_tag="ACHKCL_003847"
     CDS             1829..3076
                     /gene="tnaB"
                     /locus_tag="ACHKCL_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5764726.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3208..4383
                     /gene="mdtL"
                     /locus_tag="ACHKCL_003848"
     CDS             3208..4383
                     /gene="mdtL"
                     /locus_tag="ACHKCL_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5764727.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4358..5317
                     /gene="yidZ"
                     /locus_tag="ACHKCL_003849"
     CDS             4358..5317
                     /gene="yidZ"
                     /locus_tag="ACHKCL_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5764728.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5474..6223
                     /gene="yieE"
                     /locus_tag="ACHKCL_003850"
     CDS             5474..6223
                     /gene="yieE"
                     /locus_tag="ACHKCL_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5764729.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6245..6811
                     /gene="yieF"
                     /locus_tag="ACHKCL_003851"
                     /gene_synonym="chrR"
     CDS             6245..6811
                     /gene="yieF"
                     /locus_tag="ACHKCL_003851"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5764730.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6865..8202)
                     /gene="adeP"
                     /locus_tag="ACHKCL_003852"
     CDS             complement(6865..8202)
                     /gene="adeP"
                     /locus_tag="ACHKCL_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5764731.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8368..9033
                     /gene="yieH"
                     /locus_tag="ACHKCL_003853"
     CDS             8368..9033
                     /gene="yieH"
                     /locus_tag="ACHKCL_003853"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5764732.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9170..>9697)
                     /locus_tag="ACHKCL_003854"
     CDS             complement(9170..>9697)
                     /locus_tag="ACHKCL_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO5764733.1"
                     /translation="FDYKELAPIFEGVEEKQIKALSNQLEHIKSFKTDYDSRLNKYAR
                     DFYYFSSAFAINWEDLLKNYQEIRASIKDYDDLLREIKELIISRNKSLEDKRTLFDNK
                     RDNWNSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNILD
                     WFERYPDIVQNITQA"
BASE COUNT         2401 a         2392 c         2367 g         2537 t
ORIGIN      
        1 ttcgtgtctt tcgaggataa gtgcattatg aatatcttac atatatgtgt gacctcaaaa
       61 tggttcaata ttgacaacaa gattgtcgat caccgccctt gatttgccct tctgtagcca
      121 tcaccagagc caaaccgatt agattcaatg tgatctattt gtttgctata tcttaaattt
      181 gccttttgca aaggccatct ctcgtttatt tacttgtgtt agtaaatgat ggtgcttgca
      241 tatatacatg gcgaattaat ggatattgca gatgcaatat tcacagggat cactgtaatt
      301 aaaataaatg aaggattatg taatggaaaa ctttaaacat ctccctgaac cgttccgcat
      361 tcgtgttatt gagccagtaa aacgtaccac tcgcgcttat cgtgaagagg caattattaa
      421 atccggtatg aacccgttcc tgctggatag cgaagatgtg tttatcgatt tactgaccga
      481 cagcggcacc ggggcggtga cgcagagtat gcaggccgcg atgatgcgcg gcgacgaagc
      541 ttacagcggc agccgcagct actatgcgtt agccgagtca gtgaaaaata tctttggtta
      601 tcaatacacc attccgactc accagggccg tggtgcagag caaatctata ttccagtact
      661 gattaaaaag cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg cgttctctaa
      721 ctatttcttt gataccacgc agggccatag ccagattaac ggctgtaccg tgcgtaacgt
      781 ctatatcaaa gaagccttcg atacgggcgt gcgttacgac tttaaaggca actttgacct
      841 tgagggatta gaacgcggta ttgaagaagt tggtccgaat aacgtgccgt atatcgttgc
      901 aaccatcacc agtaactccg caggcggtca gccggtttca ctggcaaact taaaagcgat
      961 gtacagcatc gcgaagaaat atgatattcc ggtggtaatg gactccgcgc gctttgctga
     1021 aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa gactggacca tcgagcagat
     1081 cacccgcgaa acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat
     1141 ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagt ttctttgatg tgtacaccga
     1201 gtgcagaacc ctttgcgtag ttcaggaagg cttcccgacg tatggcggcc tggaaggcgg
     1261 cgcgatggag cgtctggccg taggtctgta tgacggcatg aatctggact ggctggctta
     1321 tcgtattgcg caggtgcagt atctggtcga tggtctggaa gagattggcg ttgtctgcca
     1381 gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa ctgcttccgc atatcccggc
     1441 agatcagttc ccggcacagg cgctggcctg cgagctgtat aaagtcgccg gtatccgcgc
     1501 ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg
     1561 cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga
     1621 cttcattatt gaagccttca aacatgtgaa agagaacgcg tcgaatatta aagggttaac
     1681 ctttacctac gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt
     1741 aatactactg agtggctata aggatgttag ccactctctt accctacatc ctcaataaca
     1801 aaaatagcct tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc
     1861 attttggggt gttatggtta tagcaggtac agtaatcggt ggaggtatgt ttgctttacc
     1921 tgtggatctt gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt
     1981 ttcaatgctt cattccgggt tattgttatt agaagcaaat ttaaattatc ccgtcggctc
     2041 cagttttaac accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat
     2101 taccgttgcc ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat
     2161 cattagtgaa acgatatcaa tgaatttggg ttatcacgct aatccacgta ttgtcgggat
     2221 ctgcacagcc attttcgttg ccagcgtatt gtggataagc tcgttagccg ccagtcgtat
     2281 tacctcattg ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt
     2341 cttcctggtc gattactcca ttctgcgcga tgccaccagc tccactgcgg gaacgtctta
     2401 cttcccgtat atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa
     2461 tattcccagc ctgattattt gctatggcaa acgcaaagat aagctaatca aaagcgtggt
     2521 cttcggttcg ctgctggcgc tggtgattta tctcttctgg ctctattgca ccatggggaa
     2581 tattccgcga gaaagcttta aggcgattat ttcctcaggc ggcaacgttg attcactggt
     2641 gaaatcgttc ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc
     2701 caacttagct gtcgccagct cgttttttgg tgtcacgctg ggattgttcg attatctggc
     2761 ggacctgttt aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt
     2821 cctgccaccc gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg
     2881 tgccggactg tgcgccacta tttgggcggt cattattccc gcagtgctgg caatcaaagc
     2941 tcgcaaaaag tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat
     3001 tgtcattctc tttggtataa ccgtaatttt gtgctggttc ggcaacgtct ttaacgtgtt
     3061 acctaaattt ggctaaatcc ttcaagaagc cagtcattcg ctggcttctt gcctctcagg
     3121 aaatcactta tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt
     3181 caccttacta tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg
     3241 gttttacttt atcccgctgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc
     3301 gatctcaatg ccagcgaagc gcagttacat attgcgttct ccgtttatct ggcaggtatg
     3361 gcagctgcga tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa accggtcgcc
     3421 atccccggcg cggcgctatt tattatcact tcggtgttct gttcactggc tgaaaccagc
     3481 acgttatttc ttgcagggcg ctttctacag gggttgggcg cgggctgttg ttacgtggtg
     3541 gcattcgcca ttttgcgcga cacgctggat gatcgacgtc gggcaaaagt gctgtcgtta
     3601 ctcaacggca ttacctgcat cattccggtg ttagcgccgg tgctcggtca tctgattatg
     3661 cttaaattcc cgtggcagag tctgttctgg acgatggcaa taatgggcat cgcggtactg
     3721 atgttgtctt tgtttatttt aaaagaaacg cgccctgccg cccccgcagc ttcggacaaa
     3781 tcacgagaaa atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc
     3841 accctcagcg tttcggtgat cctcaccttc gtcaatacat cgccggtatt gctgatggaa
     3901 atcatgggtt ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc
     3961 atgaccgttt cattttccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg
     4021 atcacctcac aggtgttatt cctggcggcg ggcatcactc ttaccgtttc accttcccat
     4081 gcggtttctc tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggtgtg
     4141 gcgatgagtc aggcgttagg gccgttttca ttacgcgcgg gcgtagccag ctcgacctta
     4201 ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc
     4261 agcgcatgga atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg
     4321 attatgttcg tcgcgcctgg acgcccggtt accgctcatg aagaaatcca tcaccacgct
     4381 tgatctcaat ctgctgctct gtctgcagct gctgatgcag gagcgcagcg taaccaaagc
     4441 ggcgaagcgg atgaacgtga caccttcggc ggtgagtaag tcgctggcaa agttaagagc
     4501 gtggtttgac gacccgctct ttgtgaactc accgttgggg ctgtcgccca caccgctaat
     4561 ggtcagcatg gagcaaaatc tggcggagtg gatgcaaatg agcaacctgc tgctggataa
     4621 accgcaccac cagacaccgc gcggcctgaa gtttgagctg gcggcagaat caccgctaat
     4681 gatgatcatg cttaatgcgc tgtcgaaaca gatctaccaa cgttacccgc aggcgaccat
     4741 caaattacgt aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat
     4801 cggtttttcc ggtcgcgaaa gtcatccccg ttcgcgggag ctgttaagct cgctaccgtt
     4861 agccattgat tatgaagtgt tgtttagtga tgtgccctgc gtctggttac gcaaagatca
     4921 tccggcactg catgaaaagt gggatctcga taccttttta cgttatccgc atatcagcat
     4981 ttgctgggaa cagagcgata cctgggcgct ggacaatgtg ttacaggagc tggggcgcga
     5041 acgtacgatt gcaatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcgcagcc
     5101 cgacaatctg ctcctggcga ccgcgccgcg ctactgtcag tactacaatc aactccacca
     5161 actgccgttg gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt
     5221 cccttttacc ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg
     5281 ggataccatt aaaaatcttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc
     5341 gatttcacaa taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg
     5401 ctttagtctg tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag
     5461 catggagcga aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat
     5521 ttctgttcgt ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca
     5581 aagccgtttt ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat
     5641 tggcgaattg ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa
     5701 tttgccttcg ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga
     5761 aggtgaatgt ggcctcgata tggaactgca gcgtgcgacg cgcgggtttc atagcccaca
     5821 cgcgcccgat aaccacacat tttccagcaa tgaatcgcta tgggtcagta aacaaaatga
     5881 tccaaacgaa gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg
     5941 tgatgttttg aatgacgatc cgcgcgatct gcaactgctg cccattgccg gacgcctgaa
     6001 atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc
     6061 cgtggcggtc acgcccacga ttgaaaagct cagtgtctgg gagttagatg gcaaacacgg
     6121 ctggaaaagc ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg
     6181 ttttgcccag ctctctacca tgaaggcttt ttcgccaaat tgatagacaa ccacaggagt
     6241 cattatgtct gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt
     6301 taatggcatg gttgcacgta ccctaccgaa aattgctccg gcgagcatgg aagtcaatgc
     6361 gttaccttcc attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt
     6421 tccagcaacg gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt
     6481 cacgccggaa tataactatt cggtaccggg tgggctgaaa aatgccatcg actggctttc
     6541 ccgcctgccg gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg
     6601 cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc
     6661 aatggtgatg aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca
     6721 aaccggagaa gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt
     6781 tggcgagttt attcagcgag ttaagatcta aataaaaaac ccgccagcaa tcatgcatgg
     6841 cgggttttta acgcgctatc gattttagtg agcgtcgata aatacaatct tcaggataaa
     6901 cagcagcgca acgatgatta cgcacgggct aaggtcacga atacgcccgg taccaatctt
     6961 catcacgcag taggagataa agcccagcgc aataccttcg gtaatcgaga agctgaacgg
     7021 catcatcaca gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg
     7081 agcaagactt gaggtcatca acacgccaac gtaaatcagc gcgccagctg cagcgtagcc
     7141 tggcaccatt cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac
     7201 cactgccgtc agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc
     7261 cgtaacggaa gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag
     7321 cgcctgcttc atgcgcggga atttcccctt ctcatctgcc agaccggctt tatcggtcac
     7381 gccaatcaac gtgccggagg agtcaaacag gttgaccagc atgaaagaga aaatcacccc
     7441 tgccagcccg aggttaaacg acccggctaa atctacatga cccacgacag tcattacgct
     7501 cggcggcgca gaaacgatgc cattgtagtg gacatcaccc agcatccagc ccagcagcgt
     7561 tgtcaccacg atagaaacca gcaccgctgc gtgaatgttg cgtgaggcca gaatagcaat
     7621 gatgaagaag ccgaggatcc ccagcagcac gctgtgagaa gtcagattac cgatgctgac
     7681 cagcgtttcc gggttagcga caatcacacc tgcgtttttc agccccatca tgccaatgaa
     7741 cagaccgata ccgctggtaa tgcccacacg cagactcacc ggaatgttgg caatcatcca
     7801 gtagcgaacg cggaaaatcg tcagtaacag cagaccaatc gcgccccaga agattgcgcc
     7861 catcccgact tgccacggca agcccatcgc ctgcacaacg acaaaagcga agaacgcatt
     7921 caggcccata gcgggtgcca gtgcaactgg caggttagca aacagcccca tcataatact
     7981 gccgaatgca gcaatcagac aggtagtgac gaagacggcg ctggtatcca tgccagcaac
     8041 gccaagaatt tgcgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc
     8101 ggcgatcact tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag
     8161 catcccctgg ccagaagcct gggttgtgtg ttgttgactc attatctatt tccgaacaag
     8221 gagggaaaat ccgtcgctat cgtataccaa aatgagacaa tatggacggc tgaagataac
     8281 ttttttcatt gtatttgctt atacggcaac gattgcgtca tgcgaattgg catgacgaaa
     8341 acgttaaact aacgataaag gaaagacatg tcccagatag aagcggtatt tttcgactgc
     8401 gacggtacgc tggtcgatag cgaggtcatt tgctctcgcg cctatgtcac gatgttcagg
     8461 gaatttggca ttcacgttga cctgaaagag atttttaccc gttttaaagg cgtaaaactc
     8521 tacgaaatca tcgatatcat cagtaaagaa cagggcgtga cgatggtcca ggccgatgcc
     8581 gagcacgttt accgcgcaga agtagcacgt ctgtttgata ccgaacttga ggacatcgca
     8641 ggagcgaacg aattgctggc gagcattaac gtgccgatgt gcgtcgtctc taacggtccg
     8701 gtcagcaaaa tgcagcattc gcttggtaag ctcaacatgc tgcactactt cccggaaaaa
     8761 ctgttcagcg gatatgacat tcagcgttgg aagcccgatc cggcattaat gttccatgcg
     8821 gcaaaagcga tgaatgtgaa cgttgaaaac tgcattctgg tcgatgactc cagcgctggc
     8881 gctcaatcag ggatagatgc cggaatggaa gtgttttact tctgcgctga cccgcacaat
     8941 aagccgatcg ttcacccgaa agtcaccacc tttaccgagc tgtcgcagct gcctgcgctc
     9001 tggaaggcac gcggctggaa tataacgcga taatcgcctc cttacttcaa cacttacaca
     9061 tcagtcacgc tggtttgaga tacagaacac caggtgtctg atgagtaagc gtctattccc
     9121 tatgaataat ttatgtcgtt attcgtcctg tcatccaccc tctgtatggt taagcctggg
     9181 ttatattttg tacaatatcg ggataccgtt cgaaccaatc cagaatatta cgcatcgcgt
     9241 cactgatgtt tttatcgtct ttatattgat tctcaagtct attatttcga acaatagtaa
     9301 gcttagaacg tattttatca tcacaatcga caattttcgc attcaatgca tttacttcat
     9361 cctgcacttc tatactattc caattgtccc ttttgttatc aaataaagtc cttttatcct
     9421 ctaatgattt attacgactt atgattaact ctttaatttc tcgcaataag tcatcgtaat
     9481 ctttaatgga agctcttatt tcctgatagt tttttagtaa atcctcccag ttaattgcaa
     9541 atgcactgga aaaatagtaa aaatcgcgag catacttatt gagccttgag tcgtaatctg
     9601 tcttaaacga ttttatatgt tccagttggt ttgatagtgc tttaatttgt ttttcttcaa
     9661 caccttcaaa aatgggtgca agttccttat aatcaaa
//
LOCUS       DAZFKY010000175         9595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFKY010000175 DAZFKY010000000
VERSION     DAZFKY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..764
                     /gene="espX2"
                     /locus_tag="ACHKCL_003855"
     CDS             <1..764
                     /gene="espX2"
                     /locus_tag="ACHKCL_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO5764734.1"
                     /translation="GRVMRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDD
                     PETICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNN
                     QPTELNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFCLSAVFA
                     NISHSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNE
                     EANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1048..3198
                     /gene="dinG"
                     /locus_tag="ACHKCL_003856"
     CDS             1048..3198
                     /gene="dinG"
                     /locus_tag="ACHKCL_003856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5764735.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3226..4188
                     /gene="ybiB"
                     /locus_tag="ACHKCL_003857"
     CDS             3226..4188
                     /gene="ybiB"
                     /locus_tag="ACHKCL_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5764736.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4329..5414
                     /gene="hcxB"
                     /locus_tag="ACHKCL_003858"
     CDS             4329..5414
                     /gene="hcxB"
                     /locus_tag="ACHKCL_003858"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5764737.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5481..5540
                     /gene="ybiE"
                     /locus_tag="ACHKCL_003859"
     CDS             5481..5540
                     /gene="ybiE"
                     /locus_tag="ACHKCL_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5764738.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5643..5903)
                     /gene="ybiJ"
                     /locus_tag="ACHKCL_003860"
     CDS             complement(5643..5903)
                     /gene="ybiJ"
                     /locus_tag="ACHKCL_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5764739.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6168..6434)
                     /gene="ybiI"
                     /locus_tag="ACHKCL_003861"
     CDS             complement(6168..6434)
                     /gene="ybiI"
                     /locus_tag="ACHKCL_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5764740.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6510..7196)
                     /gene="ybiX"
                     /locus_tag="ACHKCL_003862"
     CDS             complement(6510..7196)
                     /gene="ybiX"
                     /locus_tag="ACHKCL_003862"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5764741.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7238..9520)
                     /gene="fiu"
                     /locus_tag="ACHKCL_003863"
     CDS             complement(7238..9520)
                     /gene="fiu"
                     /locus_tag="ACHKCL_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5764742.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
BASE COUNT         2288 a         2348 c         2601 g         2358 t
ORIGIN      
        1 ctgggcgagt aatgcgctta tcgcaccagg cgttaatctc aatgcttgag ccagaggtta
       61 agaaaaagac tatctggaat aattactcta tttatccatc tttgcaggat acccatgaag
      121 ttgtcagaga tgatccagaa acgatttgta tgcgtgcatt tcctttattt gcaaaaggat
      181 gggaatatgc gcagaaaaat aagaaacatc aattgatcct caatgctctt ggattcaaag
      241 gttatattcg tgatatattt atgtcagcaa taatgcgaaa aacagatttt gtgctcgaat
      301 gcaataatca accgacagaa cttaattcat cattttcctc tttgatgaac gattcagatc
      361 aatggcaaca gcatactctg aaagataaac attacgctaa tctattaacg atgctggatc
      421 tcaatgatgc cagtgaaagt gataagtcta aaatattttt ctgcctgtct gccgtatttg
      481 caaatatttc tcacagtaat gtctttaacg gtattcctga tgcgtcaaaa actttaaaac
      541 gttatgcatt tgctttattg gcgaaagctc attctttaga tgaaagtatg ataagcaatc
      601 aaacttttaa tacatacaaa acggtattgc ttgatttcaa taatctgtct aatgaagagg
      661 ccaaccagtt gcgcataagt tcgttatatc gtgatatggt ccgctatgca caatatcgtt
      721 tttctaaagt tttatccgag tggacgccag acgcatggct gtaaagatcg cagcagaata
      781 atggcattga tggccctggc tcaggccagg gtcggcaata ccggttcctg cgaagcgatg
      841 acggcggcaa ttgtaaccac gaaatgcggt atcacttgct gttttctgcc ctgaagactg
      901 gcgctcactg tgccgtcgaa ttttcaatca aatatattgc gagagggcgt gtttgcgagc
      961 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
     1021 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
     1081 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
     1141 cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaagggcg gcatctggcg
     1201 attgaagccc ccactggcgt tgggaaaacg ctctcctatt tgattcccgg catcgccatt
     1261 gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca acgtggcatt gcaggatcag
     1321 atctacagca aagatttgcc actgctgaaa aagatcattc ccgatcttaa attcactgcc
     1381 gcttttgggc gtgggcgcta cgtttgcccg cgtaatctga cggcgcttgc cagcactgag
     1441 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccaaa taatcaggaa
     1501 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
     1561 cgcgatcata cggatatcgc tatagatgac gatctctggc gtcgtttaag taccgacaaa
     1621 gccagctgcc tcaaccgcaa ctgttactac tatcgcgaat gcccgttttt tgtcgctcgt
     1681 cgggaaattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
     1741 gaaagcgaag ccgtattgcc tgacccgaaa aatttactgc tggtgctgga cgaaggccat
     1801 cacctgccag atgtggcgcg ggatgcgctg gagatgagtg ccgaaatcac cgcgccatgg
     1861 tatcggctac agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc
     1921 ccgaagacca tcccgccgtt ggcgatccct gaacgtttga atgcgcattg tgaagagctg
     1981 tatgagctta tcgcctcatt aaacaacatt ctcaatctct acatgcctgc cgggcaggaa
     2041 gcagaacacc gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg
     2101 ctggcaaaac tcaccgagat gctgcgtggt ctggctgagt tatttcttaa cgatttaagt
     2161 gagaaaaccg gcagccatga cattgtacgt ctgcatcggt tgattttgca gatgaaccgc
     2221 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcttcgct ggcgcaatct
     2281 tccggtgcac cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctc
     2341 tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
     2401 attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
     2461 caggagatga gcgggctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc
     2521 tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
     2581 gacaacgaag aacagcatat tgctgaaatg gcggcctttt tccgtgagca ggtggagagc
     2641 aaaaaacatc tcggtatgtt ggtgctgttt gccagcgggc gtgcgatgca gcgctttctc
     2701 gactatgtga cggatttacg tctgatgttg ctggtgcagg gcgatcagcc gcgttaccgt
     2761 ttagttgaac tgcaccgcaa acgcgtcgcc aacggtgagc gtagtgtgct ggtgggctta
     2821 cagtcatttg ccgaagggct tgatttgaaa ggtgatatgc tcagccaggt gcatatccac
     2881 aaaatcgctt ttccgcctat cgacagcccg gtggtgatca ctgaagggga atggctgaaa
     2941 agcctcaacc gctatccatt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt
     3001 cagcaggttg ggcgactgat tcgaagtcac ggttgctggg gcgaagtggt gatttacgat
     3061 aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg
     3121 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
     3181 cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa
     3241 atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
     3301 cgcggtctgt atgcccatat gctcaatggt gaagtccctg accttgagtt gggcggtgta
     3361 ttgattgcgc tgcgtatcaa aggggaaggg gaagcagaga tgctcggctt ttacgaagcc
     3421 atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc gattgtcatc
     3481 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
     3541 cacaaactcg gttttcctgt ggtggttcac ggggttagcg aagatccaac tcgcgtgctg
     3601 accgaaacca ttttcgaact gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
     3661 gcgaagctcg acgaacatca accggtgttt atgcctgtcg gcgcgttttg cccgccgctg
     3721 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
     3781 aaactggcga cgccatttgc cgaaggtgag gcactgcgtc tttccagcgt ttcgcatccg
     3841 gaatacatcg ggcgagtcgc gaagttcttt agcgatatcg gcgggcgggc gttactaatg
     3901 catggtactg aaggcgaagt gtatgctaat ccgcagcgct gcccacagat caatctcatt
     3961 gatcgtgaag agatgcgggt gctgtatgaa aaacaggacc ctgctggaag cgagttactg
     4021 ccacaagcaa aagatccgga aaccacggcg cagtggattc agcgttgcct tgctggccgc
     4081 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctggtggc tacgggtgaa
     4141 gcggcaacta tcagcgatgg cctggcgcgc gttaatcagg cattttaatt cttttctcag
     4201 ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
     4261 ttgaccttcc cgcgcccggc gtgcagcata aaaaaacaac aaacacataa cataaacagg
     4321 agttaaccat ggaaagtggt catcgctttg atgctcagac gctgcacagc tttattcagg
     4381 ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa
     4441 tcgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca agctatgtac
     4501 gctcctggag tcaggggcac ctgcaaatta accatcatgc caaaatcgtt aaagaggcgg
     4561 gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga
     4621 tggcgctggg gattgagaaa gcgcatcagc acggtattgc tgccgtggcg ctacataact
     4681 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
     4741 ctatccactt tgttagcgtg gtcggtattc caatggtcgc accgttccac ggtcgcgaca
     4801 gccgctttgg cacgaatccg ttctgtgtgg ttttcccacg taaagatgat tttccgctgt
     4861 tgcttgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag
     4921 gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg
     4981 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
     5041 ccctggcggc aatgtgtgaa attcttggcg gggcgctttc tggcggtaaa acgacgcatc
     5101 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
     5161 acccggaact gttcggcgcg ccggattgta gcgcgcagac cgaagccttt gccgagtggg
     5221 tgaaagcctc gccgcatgat gacgataagc cgattttgct cccgggcgag tgggaagtga
     5281 acacgcgtcg cgaacggcag gagcagggga ttccattgga tgcgggaagc tggcaggcca
     5341 tttgtgatgc ggcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc
     5401 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
     5461 atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgcag
     5521 gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc
     5581 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
     5641 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg
     5701 ctatatccac tggcaccggc tgccgccgct ttctcagcca gtttcgcttc cagggcatcg
     5761 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
     5821 gccgttaccg gttccgcggc aaacacgcca aatgacagag ctgaaagagc catagcagca
     5881 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
     5941 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt
     6001 gaccgccttg ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt
     6061 gtacttatgg acttgtttta tgcagcgtgg cagtcacgac agcgttaaag gcacggaatg
     6121 ccacgcaaaa tacaaattat actcgctata catctcgcca gttgtagtta acgtaactgg
     6181 ctttctttcg aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc
     6241 tgctgacaat gaatacataa gcgcacgcca ggaatggctt cccgacgggc ttgcgggatg
     6301 ggggcaccgc actcttcaca ttcatacagg ctttcgccac gcggaatttc accccgagcg
     6361 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc
     6421 caaccggatg ccatatcgtc ctccccatat atcaatgctt gtatagttaa gtgtagctaa
     6481 tatagggacg aacctgggca gttcaagcgt cagatctccg accattcccg cagcagatta
     6541 tgataaagat tgagcagcga caggatctct tcattttcac catagcggct tttcagcgac
     6601 tgaatattct gaatattttt gtccagttca aacagcatgg cgcgcttttt atcatcgcgg
     6661 atcatcgact ggatccatat aaatgatgcc actcgtacgc cgcgggttac gggtgtcacg
     6721 caatgcaggc tgctggaggg atacaacacc agatcacctg ccgggagttt tacccgatgt
     6781 tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg atcgcttaaa
     6841 aacagcgtgg cagaaaggtc agtgcgcatc cagccgtttt gtggatggct gcgtactgcg
     6901 ccatccacat gaaaaccata ggtttcattg ttctgatagc gattaaacag cggcgtggaa
     6961 agggtacgcg gcaaggccgc ggcaaagaac aaagcatgtt ggttaaccgc gttcagcacc
     7021 tcattttgca gggcggcgta taacgtgctg cgggtgtcta cctgttgatt gttcttaact
     7081 tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg ttccagttgt
     7141 tcgcgaaaac gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta catcatacaa
     7201 gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata ttggctgtga
     7261 gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttattg attgaggcga
     7321 cgtaatcggt atcaaacagg ttgtagacgt ttagctggaa gtcgagattg cgattaaccc
     7381 tataccccag tttggcatcg gcgacccagt aaccttcggt aaacgctggc gttcccaccg
     7441 cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca acagagatat
     7501 catcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta tacggcagcg
     7561 atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt tgctgggtat
     7621 agccgccaat cacctgccac gcgggagtga tattccctgc cacggatatc tcatagcctt
     7681 cgacgcgttt cttaccgtat tgcgagtaag tcccgtcatc attttgctca acttcatttt
     7741 cgatatcagt acggaacagc gcggcggtga gcaacaggcg tttatccaga acctgccatt
     7801 tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg ttggcactgt
     7861 taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc tgggaaacgg
     7921 catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg gctttccagt
     7981 tcaccagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct tttgccacac
     8041 cagctgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca ctgtcatatt
     8101 cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg gtgatttgca
     8161 gcgtatcgaa tgcgtaaatt gcgaaggtat ccgtctgacc attggcgttt gcgccgttgc
     8221 gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc gcaggtaacg
     8281 tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg ccggtactga
     8341 catcatgacc gatagaagcg gtatagaatg tcgaggtcag gttggtctgg ttggtgagaa
     8401 ttttattact cacatctttg gtattcgccg tgcgtgacca cgtccagcta ttcacatcgc
     8461 tggtgggctg ggtaatattc gacgccccac ccataatcgc cgtcatcagg taatcctgct
     8521 tcacgcgcga ccaacgggta gtgttgcgaa tggtggtgtt atcgttgata tcgtgctcaa
     8581 aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg ccgtaaaagt
     8641 tatgcgtatc aacttttccg gaatgattca gggtcgccgt tcctgcagat ggggcagaat
     8701 agcccggcaa accgatggtt ggaatgccgc cgtctggcgt gttgtgctgg gtgacatgca
     8761 gataattaag atacaaacga ttcgctgtac caaggccaaa agcgatagaa ggggcgacgc
     8821 cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg cccatcacat
     8881 tcaggcgcac cgcagtggta tcaccaatga cctgattgac gtccagcgtg ccgcggcgga
     8941 accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc ggctgcttgc
     9001 tgatcatatt gatcgagccc gtcggcgcgc tgcgcccgta gtcggtgccg gaaggccctt
     9061 taatcacttc gacctgctca gtattgaagg tgtcgcgcga gacgctgccg atatcgcgaa
     9121 tgccatcaat ataaatactg ttagaggtat cagcaccgcg catataaatg gcgtcgccag
     9181 tggtggagtt accgttctca cccgcaaaaa acgcacccac gccggggacg tttttgagcg
     9241 catcggtaag gttggttgcg ccctgatctt taatcacttg ttcagaaatc accgtcatcg
     9301 tgcgggtagt atccgctacc ggacgcgaga atttcggatc ggcagattgt tgtggcgcat
     9361 aaagcgaagg cgttgatgct tcgacaacca gcgtgtcatc cgcgttagtt tgcccttcgg
     9421 cggcgagtgc ctgagccaca ggcgtgatgc caatacaaag accggcaaag aacgtgagcg
     9481 aatgaaattg tctggcaggg aaattgcgat tgttttccat tttgcaggtg actttttctt
     9541 atatgttgaa attttcactg ccacgagaaa gcctctctgc ggatgcgatg ccact
//
LOCUS       DAZFKY010000176         9525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFKY010000176 DAZFKY010000000
VERSION     DAZFKY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..388
                     /locus_tag="ACHKCL_003864"
     CDS             <1..388
                     /locus_tag="ACHKCL_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5764743.1"
                     /translation="RDFYISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQ
                     LKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIA
                     KGANQCARAGDKLPDLLDEYIRATTE"
     gene            360..2288
                     /locus_tag="ACHKCL_003865"
     CDS             360..2288
                     /locus_tag="ACHKCL_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5764744.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGGGGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSIMFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPSPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2272..2478
                     /locus_tag="ACHKCL_003866"
     CDS             2272..2478
                     /locus_tag="ACHKCL_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5764745.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2475..4067
                     /locus_tag="ACHKCL_003867"
     CDS             2475..4067
                     /locus_tag="ACHKCL_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5764746.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4057..5562
                     /locus_tag="ACHKCL_003868"
     CDS             4057..5562
                     /locus_tag="ACHKCL_003868"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5764747.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5599..5946
                     /locus_tag="ACHKCL_003869"
     CDS             5599..5946
                     /locus_tag="ACHKCL_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5764748.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            6004..6255
                     /locus_tag="ACHKCL_003870"
                     /pseudo
     CDS             6004..6255
                     /locus_tag="ACHKCL_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6252..6992
                     /locus_tag="ACHKCL_003871"
     CDS             6252..6992
                     /locus_tag="ACHKCL_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5764749.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            7006..7437
                     /locus_tag="ACHKCL_003872"
     CDS             7006..7437
                     /locus_tag="ACHKCL_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5764750.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7464..7877
                     /locus_tag="ACHKCL_003873"
     CDS             7464..7877
                     /locus_tag="ACHKCL_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5764751.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7858..>9525
                     /locus_tag="ACHKCL_003874"
     CDS             7858..>9525
                     /locus_tag="ACHKCL_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5764752.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKFLLAHKDETLEYKRQLAELGDKVE"
BASE COUNT         2217 a         2312 c         3003 g         1993 t
ORIGIN      
        1 gcgcgatttt tacatatcgt atctgaggag aagtaaaaac gatgtgcctg atggcgatag
       61 cgaagacgac tatgaggaga aattgcttat tgccagatgg gaactgacag cagaacaggc
      121 tgttacacag cagttaaaaa atgaggtgtc aaaaggaaaa ctgattgaca ccgggttctg
      181 tatttttgcc ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt
      241 atccatgcag cgacagtttc ctgatttaac accgcgccat cttgaccatc tgaaaaccct
      301 tattgctaag ggggcaaatc agtgtgcgcg ggcaggggat aaattaccgg atttacttga
      361 tgaatatatc agagcaacaa ctgaataata tgatgagcgc tgtcacaact gcattacagc
      421 ccctgataag ggcattgccg gtgacgccag ttgaatgggc tgatcaaaat tattatctgc
      481 ctaaagaatc ttcatatggt gagggcgaat ggaaaacgct gccattccag atcgccatca
      541 tgaacagtat ggggaatgat cagatccgca ctgttaatct gattaaatct gcccgtgttg
      601 gctatacaaa gatgttgctg ggggggggcg ggtattttat tgagcataaa tcccgaaaca
      661 gtctgctttt tcagcccacg gattctgccg ctgaagattt tatgaagtct cacgtggagg
      721 cgacgattcg gaacgtgcca tgcctgaaag acctttcccc atggctgggt cgtaaacatc
      781 gtgacaatac tctcacgctg aaacgctttt catcgggcgt cggtttctgg tgcctgggcg
      841 gcgctgccgc caaaaactac cgtgaaaaat ccgttgacgt ggtctgctat gacgaacttt
      901 cctcgttcga gccggatgtc gaaaaagagg gctcgccaac cctgctgggg gataagcgta
      961 ttgaggggtc ggtgtggcca aaatccattc gcggctcgac gcctaaaatc aaaggcacct
     1021 gccagatcga aaaagccgct aacgagtcgg cgcattttat gcgtttttat gtgccctgcc
     1081 cgcactgtgg ggaggcgcag tatctgaaat ttggcgatga gtccacgcct tttgggctta
     1141 aatgggagaa ggacagccct gaaagtgttt tctacctctg tgaacatcat ggctgcgtga
     1201 tccatcagtc tgaactggac cagagcaacg ggcggtggat ctgtgaaaac acgggcatgt
     1261 ggacccgtga cggtctgacg tttttcagcg cccggggtga tgaaattccg ccgccgcgct
     1321 ccatcatgtt ccatatctgg acggcgtaca gtccgttcac cacctgggta cagattgtct
     1381 atgactggct ggatgcactg aaagatccca acggcctgaa aacctttgtg aacaccacgc
     1441 tgggcgagac ctgggaagag gccgtgggcg aaaaactcga tcaccaggta ctgatggata
     1501 aggtggtgcg ttacacggcg gcggtgcctg cccgggtggt ttatctgacg gcgggcattg
     1561 actcgcagcg aaaccgtttt gagatgtatg tctggggatg ggctccggga gaggaagcct
     1621 ttctggtgga taaaatcatc attatggggc gtcctgatga ggaagagacg ctgttacgtg
     1681 tggatgcggc gatcaacaaa aaataccgcc atgcggatgg caccgaaatg actatttccc
     1741 gtgtctgctg ggacaccggg gggatcgatg gtgaaattgt ttatcagaga tcaaaaaaac
     1801 acggtgtttt ccgggtgctg ccggtaaaag gcgcatctgt ctatggcaag ccggtgatca
     1861 ccatgccaaa aacccgcaat cagcggggcg tgtatctgtg tgaagtggga acggacaccg
     1921 caaaagaaat tctctatgcc cgtatgaaag ccgatccctc gcctgcggat gaagccacgt
     1981 cgtatgccat ccgttttcct gatgatccgg agattttttc gcagacagag gcgcagcaac
     2041 tggtggcgga agagctggtg gagaagtggg aaaaaggaaa gatgcgtctg ctgtgggata
     2101 acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg gcattacgtg
     2161 tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg gaagaagaga
     2221 cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg aatggttaca
     2281 gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg taaacgggtg
     2341 gtgtctgtcc agaaagatgg tcgcagaatt gaatatacgg cggcttctct ggatgagctt
     2401 aaccgggcga tcaatgatgc ggagtcggta ctggggacaa cccggcgtcg ccgtcgtccg
     2461 ctgggagtga ggttatgaaa cgaacgcctg tcctgattga tgtgaacggc gttccgcttc
     2521 gtgagagtct cagctacaac gggggcggtg caggatttgg cgggcaaatg gcggagtggt
     2581 tgccaccggc gcagagtgcc gatgcggccc tgctgcccgc gttgcgtctg gggaatgccc
     2641 gggcagatga tctggtgcgc aataacggaa tagcggccaa tgcggtggca ctgcataagg
     2701 atcacattgt cgggcatatg tttcttatca gctaccgtcc gaactggcgc tggctgggga
     2761 tgcgggagac cgcagcaaaa agctttgtcg atgaggtgga ggcggcctgg tcggaatacg
     2821 ccgaagggat gtctggcgag atcgacgtgg aaggaaaacg cacgttcacg gaatttatcc
     2881 gtgaaggtgt gggcgttcat gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata
     2941 cggaaaccac gcagttattc cgtacgcgtt ttaaagccgt gagtccgaaa cgggtggaca
     3001 cgccaggaca cggtatgggg aaccgttttc tgcgggccgg ggtggaggtc gatcgatatg
     3061 gccgtgccgt tgcgtaccat atctgtgagg atgattttcc tcgctccggg agtggacgat
     3121 gggaacggat cccgcgtgaa cttcccaccg ggcgtccggc catgctgcat attttcgagc
     3181 cggtggagga cgggcagacc cgtggggcca accagtttta cagcgtcatg gaacggctga
     3241 agatgctcga ttccctgcag gcaacacagc ttcagtcggc cattgtgaaa gccatgtatg
     3301 cagcgacgat tgaaagtgac cttgataccg aaaaggcctt tgaatatatc gccggtgcgc
     3361 cgcaggggca gaaggataat ccgcttatta atattctgga gaagttctcc agctggtatg
     3421 acacgaataa cgtgacgctg ggtggtgtca aaattccgca ccttttcccc ggggatgatc
     3481 tgaaactaca gactgcgcag gattcagaca atggattttc ggcgcttgaa caggcgctgc
     3541 tgcggtatat cgccgccggt cttggcgttt cctacgaaca gttgtcccgt gattactcga
     3601 aggtcagtta ttcaagtgcc agggcctctg ccaatgagtc gtggcgctat tttatggggc
     3661 ggcgaaaatt tattgcggcc cggctggcca cgcagatgtt ttcctactgg ctggaagagg
     3721 cacttcttcg ggggattatc cgtccgccac gggcgcgttt tgatttttat caggcgcgat
     3781 cagcctggtc acgggcagag tggattggtg ccggaagaat ggccattgac gggctcaagg
     3841 aggttcagga atcggtgatg cgcattgagg ccggactgag cacgtatgag aaagagccgg
     3901 cgctgatggg cgaggattat caggacattt tccgccagca ggtcagggaa tctgcagagc
     3961 ggcaaaaagc cggactctca cgtccggtgt ggatagcgca ggcgtatcag cagcagatag
     4021 cggagagtcg caggccggaa gaggagacaa caccccgtga gacgtaatct ttcacacatt
     4081 attgccgcag cattcaatga accgctgctt ctggagcccg cctatgcgcg ggttttcttt
     4141 tgcgcgctcg ggcgcgagat gggggcagca agtctttcgg taccacagca gcaggtacag
     4201 cttgatgctc ccggaatgct ggctgaaacg gacgagtaca tggccggagg taaacgaccg
     4261 gcccgtgttt accgggtggt gaacggtatt gctgtactgc cggtgaccgg cacgctggtg
     4321 caccggctgg ggggtatgcg gccattttcc ggaatgacag gctatgacgg cattgtcgcc
     4381 tgtcttcagc aggcaatggc ggatagccag gtgcggggcg tactgctgga cattgacagt
     4441 ccgggcgggc aggccgccgg cgcgtttgac tgcgctgaca tgatttaccg cctccgtcag
     4501 cagaagccgg tctgggcact gtgcaatgac acggcctgtt ctgcagccat gctgctggcg
     4561 tcggcctgct cccgacggct ggttacccag acatcccgta tcggctccat tggcgtgatg
     4621 atgagccatg tcagctatgc cggtcatctg gcgcaggccg gtgtggatat cacgctgatt
     4681 tactcagggg cgcacaaggt ggatggcaat cagtttgaag ccttaccggc agaggttcgc
     4741 cagaacatgc agcagcgcat tgatgcggcg cgccggatgt ttgccgaaaa agtggccatg
     4801 tttaccggtc tgtctgttga tgccgtcacg ggaacagagg ccgccgtttt tgaaggtcag
     4861 tccggcattg atgccgggct ggcggatgaa ttagtcaatg cgtcggatgc catcagtgtg
     4921 atggccacgg cgctgaacag taatgtcaga ggaggcacta tgccgcaatt aactgcaacg
     4981 gaagccgccg cgcaggagaa ccagcgagtg atggggatcc tgacatgcca ggaagcgaaa
     5041 ggacgtgaac agcttgccac gatgctggca ggacaacagg gcatgagcgt tgaacaggcc
     5101 cgggcgattc tggccgcggc ggcaccgcag cagccggtgg catccacgca gagtgaagcc
     5161 gatcgcatta tggcgtgtga agaagcgaac ggtcgtgaac aactggcggc aacgctggcg
     5221 gcgatgccgg agatgacggt ggaaaaagcc cgcccgatcc tggctgcttc accgcaggcg
     5281 gatgccggac cctcactccg tgatcagatc atggcactgg atgaggcaaa aggggctgag
     5341 gcgcaggctg aacagctggc tgcctgcccg ggaatgactg tggagagcgc ccgggctgtg
     5401 ctggctgcgg gatcaggtaa ggcagaaccg gtctctgcat ccacaaccgc cctgtttgaa
     5461 cgcatcatgg cgaaccattc accggctgcg gtacagggtg gcgtgccaca gacgtcagca
     5521 gacggtgatg cggacgtgaa aatgctcatg gccatgccat gaagtcagtg ctgaccatca
     5581 acaggaggtt tttacaatat ggtaacgaaa aacatcactg aacagcgtgc ggaagtacgt
     5641 atttttgccg gtaatgatcc ggctcatacc gccacaggca gcagcgggat ttcctcggca
     5701 acaccggcac tgacgcccct gatgctggat gaagccagcg ggaaactggt ggtctgggac
     5761 ggacagaaag ccggtagtgc agttggcata ctggtactgc cgcttgaagg cacagagacg
     5821 gtactgacct attacaagtc ggggaccttt gcgacggagg caatccgctg gcctgaaagt
     5881 gtggatgaac acaaaaaggc aaatgccttt gccggcagtg ccctgagtca cgcggcgctg
     5941 ccgtaacacg ttatcaggcc accgcggtgg cctgactgat ttctgaatga aaggaactga
     6001 tttatgggat tgtttacgac ccgccagtta ctcggttata ccgaacaaaa agtgaaattt
     6061 cgtgcgctgt ttctggagct gtttttccgc cgtacggtga atttccatac cgaagaggtg
     6121 atgctggaca aaattaccgg aaaaacgccg gtggcggcct atgtctcccc ggttgttgaa
     6181 ggaaaagtgc tgcgtcatcg cggtggtgaa acccgcgtgt tacgtccggg ctacgtcaag
     6241 ccgaaacacg aattcccctg gagccggtaa aaggagccgg taccaccctg tgggtttata
     6301 acggtcaggg tgacgcctat gcaaacccgt tgtcagacga tgactggcag cgactggcta
     6361 aggtgaagga tctgacgccg ggcgagatga cggcagaatc ctacgatgat aactacctgg
     6421 atgatgaaga cgcggactgg accgcgaccg ggcaggggca gaaatctgca ggtgatacca
     6481 gttttacgct ggcctggaaa ccgggagagg aaggccagaa agggcttata ggctggtttg
     6541 aaagcggcga tgtccgggcc tataaaatcc gttttccgaa tggcacggtg gatgtgtttc
     6601 gtggctgggt cagcagtatc ggtaaggccg tgacggcgaa agaagtgatc acccgcacgg
     6661 tgaaagtcac taacgtgggt aaaccttctg tagcggaaga acgcagcaaa attacgccgg
     6721 tcagtgcgat taaggtgacg ccgacatccg gtacggtggc aaaagggaaa acaaccaccc
     6781 tgacggtttc ttttgagccg gaaagtgcaa cagacaagac gttcagagcg gtttccgccg
     6841 atccgtcgaa agccaccatt agtgtgaaag atatgacaat tacggtaaac ggcgtggcga
     6901 caggtaaggt gcagatccct gtggtgagcg gaaatggtca gttcgccgca gtggctgaag
     6961 tcaccgttac tgaagcgggc gctgcagggt aaacggaggt catacatgtt tctgaaaaca
     7021 gaacaatttg aatataacgg tgtgtctgtc acgctttccg aattgtctgc gctgcagcgt
     7081 atcgagcatc ttgccctcct gaaacggcgt gcagaacagg cagaatccag cggcaacctg
     7141 caggtaagcg tggaagatct cgtcagaacc ggcgcgtttc tggtggcgat gtccctgtgg
     7201 cataaccatc cacagaaaac gcagtcaccg tcaatgaatg aggccgtgat gaagatagag
     7261 caggaagtgc tcaccacctg gcctgccgat gccattgccc gggcggaaga cgtggtgttg
     7321 tgcctgtccg ggatgagcgg ggctgttcgt ccggatactg atattactga agtggcgaaa
     7381 aataacacgc tgactgatga tgatttttct gcgggaaagt cttcgacggc gagctgaact
     7441 ttgccctcag actggcgcgt gagatgggga gacccgactg gcgcgccatg cttgccggga
     7501 tgacatccac cgaatatgcc gactggcacc gtttttaccg cacgcattat tttcaggata
     7561 cccagctgga tatgcatttt tccgggctga cgtacgctgt actcagcctg tttttttgcg
     7621 atccggatat gcatccctct gatttcagtc tgcttgtccc ccggcatgag gaagagcagg
     7681 tggagaggcc ggatgaggac aaaatgctga tgcagaaagc ggcaggactt gccggaggcg
     7741 tccggttcgg tggggacgga gggcgcgata ttttatcgtc tgcggatgtg gcggatgtca
     7801 tggtggatga tgccgcatta atgatggctt cagcggggat tccgggaggt gtgagatatg
     7861 tcccagccgg ttggtgatct tgttattgac ctgagtctgg atgctgtccg tttcgatgag
     7921 cagatgagcc gggtaaggcg tcatttttca ggtctggata ccgacgtcag aaaaaccgcc
     7981 agtgctgttg aacagggcct gagccgccag gcgctggctg cacaaaaagc cgggatttcc
     8041 gtcgggcagt ataaagcggc catgcgaacc ctgcccgcac agtttacgga tatcgccacg
     8101 cagcttgccg gtggtcagaa tccctggctg atcctgctgc aacagggcgg tcaggtgaag
     8161 gactccttcg gcgggatgat ccccatgttc agggggcttg ccggtgcgat caccctgccg
     8221 atggtcgggg tcacctcgct ggcggtggcg acaggtgcgc tggtgtacgc ctggtaccag
     8281 ggagattcca cgctttcagc gtttaataaa accctggttc tttccggtaa tcagtccgga
     8341 ctgactgccg atcgcatgct gacgctctca agagccgggc aggcagcagg gctgacgttt
     8401 aaccaggcga gagagtcact ggcagccctg gtgaatgccg gtgtgcgtgg tggtgaacag
     8461 tttgatgcca tcaaccagag tgtcgcgcgt tttgcttctg catccggtgt ggaggtggac
     8521 aaggttgcag aggctttcgg aaaactgacc accgacccta cgtcggggct gattgcgatg
     8581 gtgcgccagt tccgtaacgt gacggcagag cagattgcgt atgttgcgca gctgcagcgt
     8641 tccggtgatg aggccggggc cttacaggcg gcgaacgata tcgccacgaa aggctttgat
     8701 gagcagaccc gtcgcctgaa agaaaacatg gggacactgg agacctgggc ggataaaacc
     8761 gggaaggcat tcaaatcgat gtgggatgcc attctggata tcggtcgtcc tgagtcctca
     8821 gcggatatgc tcgccagtgc acagaaggca tttgatgagg cggataaaaa atggcagtgg
     8881 taccagagcc ggagccagcg ccggggaaaa accgcctctt tccgggccaa ccttcagggc
     8941 gcatggaatg accgggaaaa tgcccgtctg gggctggcag cggccacgct gcagtcggat
     9001 atggaaaaag ccggtgaact ggccgccagg gaccgggccg aacgggacgc atcacagctg
     9061 aagtataccg gagaggcgca gaaggcgtat gagcgtctgc tgacgccgct ggagaaatat
     9121 accgcccgtc aggaagaact gaataaggcc ctgaaagacg ggaaaatcct gcgggcggat
     9181 tacaacacgc tgatggcggc ggcgaaaaag gattatgaat cgacgctgaa aaagccgaag
     9241 tcgtcaggag tcaaagtgtc agccggtgag cgtcaggaag accaggcgca tgctgccctg
     9301 ctggcgcttg aaaccgagct caggacgctg gaaaaacaca gcggtgcgaa tgagaaaatc
     9361 agccagcagc gtcgcgattt atggaaagcg gaaaatcagt atgcggtcct gaaagaggct
     9421 gccacgaaac ggcagttatc tgagcaggaa aaattcctgc tggcgcataa agacgagacg
     9481 ctggagtaca aacgccagct ggctgagctg ggagacaaag ttgaa
//
LOCUS       DAZFKY010000177         9449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFKY010000177 DAZFKY010000000
VERSION     DAZFKY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(280..1329)
                     /gene="yahK"
                     /locus_tag="ACHKCL_003875"
     CDS             complement(280..1329)
                     /gene="yahK"
                     /locus_tag="ACHKCL_003875"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5764753.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1416..2372)
                     /locus_tag="ACHKCL_003876"
     CDS             complement(1416..2372)
                     /locus_tag="ACHKCL_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5764754.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(2369..3340)
                     /locus_tag="ACHKCL_003877"
     CDS             complement(2369..3340)
                     /locus_tag="ACHKCL_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5764755.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3333..4817)
                     /locus_tag="ACHKCL_003878"
                     /pseudo
     CDS             complement(3333..4817)
                     /locus_tag="ACHKCL_003878"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            complement(4866..5849)
                     /locus_tag="ACHKCL_003879"
     CDS             complement(4866..5849)
                     /locus_tag="ACHKCL_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5764756.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(6103..6513)
                     /locus_tag="ACHKCL_003880"
     CDS             complement(6103..6513)
                     /locus_tag="ACHKCL_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5764757.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(6812..8194)
                     /gene="yahJ"
                     /locus_tag="ACHKCL_003881"
     CDS             complement(6812..8194)
                     /gene="yahJ"
                     /locus_tag="ACHKCL_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5764758.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(8204..9154)
                     /gene="yahI"
                     /locus_tag="ACHKCL_003882"
     CDS             complement(8204..9154)
                     /gene="yahI"
                     /locus_tag="ACHKCL_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5764759.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(9204..9449)
                     /gene="yahH"
                     /locus_tag="ACHKCL_003883"
                     /pseudo
     CDS             complement(9204..9449)
                     /gene="yahH"
                     /locus_tag="ACHKCL_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahH"
BASE COUNT         2398 a         2598 c         2194 g         2259 t
ORIGIN      
        1 gcatcaaatt attgtgcgga gcttttaatg atatcatcgc gatctctctt aaataaaaat
       61 aaacgtcgca gataataaag ctctgattaa accaggcact atcagaaatc gctcataact
      121 aacgtgtgaa gtattaggta ctggagggag ttaatttaac aatttgaatt tacgttatag
      181 gttttactgc tactttgata ttatttcgct ctccttaact aaaaagttaa gtggagagcg
      241 aagttaatat gttaaaccac agcgtattta ttaatttttt cagtctgtta gtgtgcgatt
      301 atcgataaca aaacgatatt tcacatcacc acgcagcatt cgctcatagg cttcattaat
      361 ttgatcggcc cgaatcatct ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag
      421 catctcctga gtttctggaa tgccgccaat catagaaccg gctatcgcac ggcgtttcat
      481 gatcaggttg aaaacttccg gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat
      541 ggtgccatca cgcttcagca aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt
      601 attcaaaatg aaatcgaaac tcttcagatg agccgccatc tcatcggcat tgcgtgagtt
      661 aacaacttca tcggccccca gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa
      721 tgccaccaca tgtgccccca tcgcgtgggc cagcttaatc cccatatgtc ccagaccgcc
      781 gatgccgacc acgcccactt ttttacccgg cccggcctgc cagtgacgta gcggcgaata
      841 cgtggtgatc cctgcacaca acaaaggagc caccgccgcc agctgctctt gcgggtgacg
      901 aatacgcaga acatatcgct catgaacgac gatctgttgt gagtagccgc ccagagtatg
      961 gcccggttcg tccggcgtcg gcgagttata ggtgccggtc atgtgatcac agtagttttc
     1021 cagcccgtct tcacactctt cgcaatgttt acaactgtcg acaatgcagc cgacaccgac
     1081 caaatcgccc ggcgcatgtt tttctacctg atcaccaacg gctaccacac gccccacaat
     1141 ttcatgaccc ggcacgcagg ggtaaaccgt ccccgcccac tcggaacgga cctggtggag
     1201 atcggaatgg cagacgccac agtaagcgat ttcgattttg acatcattcg gtcccggttc
     1261 acgccgggtg atatccatcg gttcaagtgg ttgtttagcg gaatatgaac caacagcttt
     1321 gatcttcatt gtgtttactc ctgattagct atgtgtattt ggcgacgcct gatatgtcat
     1381 cgcggcaaaa cgcgtccatt gaatatagcc aatatttaaa ccacccgatc cagccccaac
     1441 atgccgcgag cctgaatgaa acagagcagc aacgttcccc ataaggccat cgtcaggtag
     1501 gggcttatac cgagaatatt gaagccgctt tcaataattt gcagaagaaa cagcgccagc
     1561 accataccaa ttatccgccc gctgccgccg tccggattaa ccccgccgag tactgccgcg
     1621 aggattgaca ccagcaagta cgattcgcca taagaggcct tagccgagtt gagtttcgac
     1681 atcatcaaaa atgccgctac cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc
     1741 acccgacgag tgttaatgcc ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa
     1801 atggctttac cgagcggcgt tttctccagc aatatccaca gccccagtgc cactgcggca
     1861 aacaaccaca tcggtagcgg aataccgaac cattgcgcat gattcaacca cagcacccat
     1921 gagggataat tggcaatggc gctaccacct gtcaccagaa tatttatccc tttgagcaac
     1981 gtcatcatcc caagcgttgc aagaattggc aacactcgaa taccagcaat caatacgccg
     2041 ttacataaac cgattatcac cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta
     2101 gcaatacccg gtggatactg agtcgccacc cacgccatca ccagtgaaca ggcgttagca
     2161 gtggctataa ttgacaagtt aatgccaccg caaagcatgg tcaccgccat agccaacgcc
     2221 agaatgccca gcacgggcat ttgcgaagcg acggactgga aattacttat cgaccagaaa
     2281 atctccggca tagtaatgct gaacgcggca acagttagca ccagcaggcc gatcaagtaa
     2341 aactcaacgt tattacgcca cgattttttc atagcaggct ccggttctga tgctgactcc
     2401 acgccgtggc gctaatgctg gcaacgatga tgatgccggt gatcaatgtt tgccagtaag
     2461 acgagactcc caataaattt agcccgtttt gcatcactgc caacaacacc acgccgagca
     2521 aagtaccagt taacgtgccg cgcccgccga gcaaactagt gccgccaagc accaccgcag
     2581 ccagtactgt cagctcataa cccagaagag aatcgggggc gacagtcatc accgtccacg
     2641 actgcactac acccgcagcg ccagacatca atcccatata gccgtagacg aaaagttgca
     2701 gtttcagcac gctaaagccg atgcgactgg ctgattcgcg gttcccgcca agggcataaa
     2761 ttttgcgccc tacactggtg aaattcatga taaacgctgt cagcagcacc accgtaatca
     2821 tggcgatcag cggcagacca aggccatagt catagccatc agcatcggtg tacttgaaca
     2881 acataacccc ctgctcaaac cacggcggaa agtcgtacag ccacacacct ttactcaacc
     2941 ataacagcag gccatagaaa atattcagcg tcgagatagt gatgatgata gaaggcaccc
     3001 gtaggcgatt aaccagtaag gcgttaatta agccaagtaa aatgccgatg cagcctgcca
     3061 gtgcaaacgc gacagcgaag ttgccaccaa ttttttgcag caacagcacc atgccgtatt
     3121 gcgcaatgat ggtcattgct ggaaacgaaa tatcaattcc accggaaatc aacaccacaa
     3181 acaaaccaca ggcgagaata gtcaacatgg catagttatt ggcgagatcg tacaaattac
     3241 cgaatgtcag aaactcgtcg ctgcgccagg caaggccaac aaacagcacc acaatcagta
     3301 atgccagcca gaattcatgg cgttttttca gttcagccat ttaccacctc cgcaatcctg
     3361 gcctgagaac tgctgtcagg caggaagcta tgggtgatct gacctttttg cattaccaaa
     3421 atccggtggc tttgatacca cgcttcttca atttcatcgc agatcatcaa taccgcaatg
     3481 ccatgagccg ccagatcgct gataatgccg tatattcctg ctttgttggc gatatcaaca
     3541 ccgactgtcg ggctatcgag aatcaacagt cgtggcccaa tcgccagcca tttggcgatc
     3601 gatactcgct gcgcgttacc accagaaagc gtattcaccg gaagatgagg atcagagact
     3661 ttgatggtca atgccttgat tagcctgtcc accaggtcac gcactttagt ttcactcaga
     3721 aagccgaagc gatcttttac cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat
     3781 tgcgccatca ccaaaccgcg cgacatgcga tcttccgaaa cataaccaat accgtggcgg
     3841 atggcgtcct ggttactatg cagtgtaacc agctggccgt tgatgaggat ctcgccagca
     3901 tctggtcggg tcataccaaa cagactcaga cataattccg tgcgccccgc accaagcagg
     3961 ccaacaatcg ataccacttc gcccgcttcc acccgcaagt taatatttag atattcccca
     4021 tgacgactta aattacgcac ctccagcacc ggggtgctgg attttccctg ccacaactct
     4081 cgcacctgat attcaaactc ctggccggtc atcaggaaac cgagttgttt tgtggtcatc
     4141 tctgcagccg gaaaagtccc gaccagctca ccatctttca gcactgaaat acggtcggaa
     4201 acttccatca cttcttccag acgatgactg acaaagacca cgcagatccc gcgttcacgc
     4261 aattgatgca ccacctgtaa taacccttgc acttcctgat gggtcagcga agcggttggt
     4321 tcatccatca cgatcaaccg tgcgtcctgc gccagtgcac ggcaaattgc caccagctga
     4381 cagcgggcaa ttgaaagctc agaaacgagc gtatccagtg gcaatgtgac gttaatacta
     4441 gtcattgccg cctgcgccac ctcacgcaga cgacggcggt taacaaacag accgtggtga
     4501 tagtgattca cggcgatatt ctcccagaca ctcaggttag gaaataaaga gaggtcctaa
     4561 taaatcacct gaatcccctg cgccaccgaa gcggcgggtg tcagctttga ccagtttttc
     4621 ccaccaagcg taatttccgc tccctcatcc gggcgataaa caccggacat aattttaatc
     4681 aacgtcgatt taccacagcc gttttgcccg gcaaggcagt gcacttcacc cgacataaag
     4741 tcgagagaaa catcgcgcag cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg
     4801 gaaaggaagg tttccataac atcccctggc aataagaatc ggggctttcg ccccgaagaa
     4861 atggattaat aaagtgagtc gatattgtct ttgttgacca gtaatacctt atggaaacgg
     4921 ataattcgct tatccatatc tacgtcggcc ttacccagat tctgcatttc taatccaggc
     4981 ttgatctctt cacccttcag caacgtgctg gcgacggctg cgagtgcgta acctgcagag
     5041 gccggatcgt aagtaatccc ttcggtgata tcaccacttt taatcagtga tgccgcctgt
     5101 gaagggatca tcatgccata gactgcgact ttatttttcg cccgtttctc tttcaccgca
     5161 cgtcccgcgc caatcggacc gtttgaacca aaggagacaa ccgctttcaa gtcaggatag
     5221 gttttcatca ggtccagtgt agtacgacgt gagacatcca cactctcggc aaccggcatg
     5281 cggcgggtaa cttcatgcat atccgggtaa tgctctttct ggtatttcac cagcaagtca
     5341 gcccataagt tatgctgcgg cacggtcaaa ctacccacgt aaatcacata gccgcccttg
     5401 ccacccatgc gtttcgccat atgctcaaca tattcagcgg caaatttttc gttatcaatg
     5461 atttcgatat cccagttagc acttggctga ccgggggatt cgttggtcag aaccacaatt
     5521 ccggcatctc gcgctttttt gaataccggt tccagcacgt tggcatcgtt tggcacgata
     5581 gtaattgcat taaccttacg ggcgattaaa tcctcaataa ttttaacttg ttgcggagca
     5641 tcagtacttg aaggccccac ctgtgaggca ttaacaccaa aggctttacc cgcctcaacc
     5701 acaccttcgc ccatgcgatt aaaccacggc ataccatcga ctttagaaat attcaccacg
     5761 actttttccg ctgcctggag cggcgcagaa attagcgcag cgcctaataa cagcgaagac
     5821 accatattga taacaaaacg tttattcatc atttcagcct cattgcaggg tagtcaggaa
     5881 gtgttcgccc aaattagcgc gctggagaga ataagaattt tcgaatattt ttatgcagaa
     5941 taaacagctc tatttcaggc agctattaaa aatatggtta taaatatgga caaaacgggc
     6001 agcaataata agtgtgacat tcatcacgta aaaaacagca aaataaacaa aattagcgat
     6061 attttatgtt tgtgagctta tttcatgtag ataaatagtt atctacacaa cgtcaataag
     6121 ggcatattct tcaaagataa cgtccatttc tggtgaatag cttccttctg cctggaaaaa
     6181 acgacggtgt tcattacgcc aatattcaag actgaggtca ccttcacctt ccttacgggc
     6241 aagctccgcg gtgacatcag aaaagcgcag taaatgcagg ccaatcactc tgataacaca
     6301 tacaggctca ccacggctat tttctacaat cttatactca ccaatcatcg gaaatgcgtt
     6361 gtcttcaata catcccgcca gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa
     6421 cgttgcgagg tgatcggcca acgcggctga atcgccaaaa gtacaaaagg aagcatgggg
     6481 atatttttgt gagaattttt cttttatggt catggcaata tttttgcgat tacggaaaaa
     6541 caaatcacat cttaattact gttggtaacc atcagcaaga gtatcattgg tttttcgata
     6601 ctgaatcttt tttatccgaa gcaggtcaca tttatggcct gataaatacc tttgttaaaa
     6661 ctcacttttg tgtatttctc tcaaataatc ccctggaaat tatgccggga gattataaag
     6721 ttcaggatca ttcattagca aaccattccc gccccactcc ctggggctta aatctcttac
     6781 tattcaccgt tgcaggcgtt tcaaatgcgc atcaacctgc cacactcccc cacaccagtt
     6841 gccctttatg gaacgttgcg gttctcggcg agatacgcgc caccgcctcg gcggaacagg
     6901 aggcgtccac cagcacaaag ctggcgtcat cctgcgcttt tggccataca cgctcgcctt
     6961 tttcattcag cggcaataca tcgccagtgg ctagaaacag cgaacgggag aggttctgtt
     7021 cgttaggacg aatatagagc tgcgcgtaga gattggcttt ttccagcatg tcgcccagac
     7081 cataaggcga ccagtggtcg ataacgctgt cagtgccagt catcactttt acgcctttgt
     7141 cgtgcaactg tttgagcggc atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa
     7201 tttgctgcgc cgccatgcga tgcgccagtt catctacctg ttgctcgttg agcgtagcca
     7261 gcgcaaaggc gtggctgatg gtcagcttac ctttcagttg aggcgttttc tctaccgttt
     7321 caaccatata attgatggct gccacgcccg acggagtggt ttcgtgcagg tgaatatcga
     7381 cgcctttgtc gtagtccagc gcaatctgga acatggtgtc gagggatttt tccatcgcgc
     7441 catcgacact ggtcgggtcc agcccgccga cgtaatgcgc acccgcctgc attgcttcac
     7501 gcattaaggg ttccgatttc gacagcagca aaccgtgctg cgggaaggcg acgatttcac
     7561 actcaaagcc cgcctgacgt cgcgccagca ccgcctgcaa attttgcaga tttttcaggc
     7621 cggaaaccgg ttcgatattg cagtggctgc gggcaatggt ggtgcctttc gactgcaata
     7681 aatcaatcag tttttctgcc cgctcctgag tgtacggttg cagctccggc agcattttct
     7741 gctcgagttt gatcatgtcc tggatggtgg tgcctgccgg gcgattgagc gagcgccacg
     7801 gcccgccgta aaacgttttg tcgagatgaa tatgcatgtc gcgggtggtg ggcagcatca
     7861 gcttatcgcc agcgtcatag tgcggcagcg tggcatccgg gtgctgctta ttctcgcgca
     7921 gcgcgacaat tttgccgttc tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg
     7981 ccacgccatt ttcgtagtca aaaccggttt ccagcagcac gttgtcgaga tagtaatgcg
     8041 ggtcagtgat tttcatggtg ttgttcgctt cgcagtttgt ggtgccagag accgccgcat
     8101 gggcgagcgg cacagaggta ccaaacagcg cggcggcggt gaccatctta ccgctctggc
     8161 tcagaaattc acggcggcta ttattttctt tcatcttacg tccttacgtt ttaataatgt
     8221 gggtgcccgt ttcaccgcgc agcgccgctg gcaggcattc cggcgtggtg ataatcactt
     8281 ctttaccgcc ctgttccagg aacgtcaggc tggcgatgat ttttggcaac atgctgccgg
     8341 gcgggaaatg tccttcctgc atatagcgag tcatggtggc aatatccacc cgatccagcg
     8401 cctgcttctc gggtttacca aagtggatac agactttttc gacgccggtg gtgatcacca
     8461 gaatgtcggc gtgaatttca cgggccagca gcgcggtaga gagatctttg tcgataaccg
     8521 cgtccacgct ttggtaatct cccgcgtcag tacgcaccac aggaattccg ccgccgcccg
     8581 cgccaatcac cacaaagcct tgttggatca gcgccttaat ggcgggtgct tcgacaatac
     8641 gtttcggttc cggcgaggcg accacgcggc gatagccccg tccagcatct tcaacaaaac
     8701 gccagtcagg gtttgccttt tgtaattcgt cacgctggct ttcactgaag aatgcaccaa
     8761 tgggcttggt gggatgggca aaacctggat cgtttttatc cacttccacc tgagtcacca
     8821 cggtgacggc ttttttctcg ccgtgacgcg ccagccggtt gttcagcgcc tgttggatca
     8881 ggtagccaat gccgccctgc gtatctgcca cacagttcgc cagcggcgtt aagggcagcc
     8941 cttcgcgctc gtgggcaatc tccgcacggc gtaaatccag cccgacctgc ggcccgttac
     9001 cgtgggtcag cacaatgtcg taatcggaag ccagcatttc cagcaccgta tcggccacgg
     9061 ctttcaccgc ctccgcctga tgctcaatcg actggctggc gttatctttg ataatgctgt
     9121 tgccaccaat ggcaacgacc acaagttctt tcatgttgtt atgtccgcta cagagggtga
     9181 tggggttatg accaacgcca gactgcctga tgcgctacgc ttatcaggcc tacaaattct
     9241 cgtgccattc gtaggccgga taaggcgttc acgccgcatc cggcaatggt gacaaatgcc
     9301 tgatgcgcta cgcttatcag gcctacaatt ctcgtgccat tcgtaggccg gataaggcgt
     9361 tcacgccgca tccggcaatg gtgacaaatg cctgatgcgc tacgcttatc aggcctacag
     9421 attcttgcgc cattcgtagg ccggataag
//
LOCUS       DAZFKY010000178         9440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFKY010000178 DAZFKY010000000
VERSION     DAZFKY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(543..1073)
                     /gene="yodB"
                     /locus_tag="ACHKCL_003884"
     CDS             complement(543..1073)
                     /gene="yodB"
                     /locus_tag="ACHKCL_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5764760.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1416..2066)
                     /gene="zinT"
                     /locus_tag="ACHKCL_003885"
     CDS             complement(1416..2066)
                     /gene="zinT"
                     /locus_tag="ACHKCL_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5764761.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2323..2958)
                     /gene="msrQ"
                     /locus_tag="ACHKCL_003886"
     CDS             complement(2323..2958)
                     /gene="msrQ"
                     /locus_tag="ACHKCL_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5764762.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(2959..3963)
                     /gene="msrP"
                     /locus_tag="ACHKCL_003887"
     CDS             complement(2959..3963)
                     /gene="msrP"
                     /locus_tag="ACHKCL_003887"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5764763.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4071..4484)
                     /gene="hiuH"
                     /locus_tag="ACHKCL_003888"
     CDS             complement(4071..4484)
                     /gene="hiuH"
                     /locus_tag="ACHKCL_003888"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5764764.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4617..5288
                     /gene="hprR"
                     /locus_tag="ACHKCL_003889"
     CDS             4617..5288
                     /gene="hprR"
                     /locus_tag="ACHKCL_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5764765.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5288..6646
                     /gene="hprS"
                     /locus_tag="ACHKCL_003890"
     CDS             5288..6646
                     /gene="hprS"
                     /locus_tag="ACHKCL_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5764766.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6754..7605)
                     /gene="hchA"
                     /locus_tag="ACHKCL_003891"
     CDS             complement(6754..7605)
                     /gene="hchA"
                     /locus_tag="ACHKCL_003891"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5764767.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8197..9383)
                     /gene="ompC"
                     /locus_tag="ACHKCL_003892"
                     /pseudo
     CDS             complement(8197..9383)
                     /gene="ompC"
                     /locus_tag="ACHKCL_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2604 a         1965 c         2182 g         2689 t
ORIGIN      
        1 ccgccttgct tttcctaaac agaacaatcg tagaatattc ttgaagggtt agatcgtcac
       61 tgttttctgt tcgatactgt gacattcagc acttgatttg ctatggatct gacaggaagc
      121 tttcgagcga aaatgtgcag ttattcagtc gttttcttat cgctcaccat tattctttta
      181 gacattcatc ctatgaaact gccgcaaagt tggtggtggg aactgaagtt gcgtagagaa
      241 ggggtcaaga attgagtcaa aaaaggaacg cggccgtcag attaaagccg cgggacaaag
      301 tccataattt ccaagagttg ttgaatttcc cagctttttt gaaagccttt gtatcatact
      361 aacaagtaaa aaaggtttaa ataccaaaat taacctgtat ttttgataag tcttaccatt
      421 gtacgattta cacattttct gctcctcctt gttgatagtg gaaagagtgc ggtatgcact
      481 tgcgtaaaca tttatgatgc gttgatgtcc atggttcaca tttcctttgt cagaggatcg
      541 acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat gtagtgatga
      601 aagagtgctg cgccagcgtg agctacgatt aaaaagtagc ctatatttgc ccaggtttcg
      661 tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg agacacattg
      721 aaaccaagga aactccacga ttttccactg taagccatca aagcaatccc cagcagagga
      781 agcgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt catttgccag
      841 gcaggtggcg gtggcacaat agaaggatca ctataacggt gttttataat caggcgtgaa
      901 aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat cagcagataa
      961 gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt tattgcaacg
     1021 aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt catttttgta
     1081 gctcctgtaa ttttcacagt caagctatcc cacgaacctt aaaaattcct tatacagatg
     1141 tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca gccaatataa
     1201 gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg aatccgttat
     1261 tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg atgagcgtgt
     1321 ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt atgatgacat
     1381 actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat ttcctcgacc
     1441 acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat ttcatttaat
     1501 aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt tcgtggtgca
     1561 attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc tttacattcg
     1621 aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta tccatcgtaa
     1681 tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc gtcctcaatg
     1741 ccaatcatct cgatatctgt tgcatagcct ttgtgataat aatctttaat ttcagcaaat
     1801 gttttagttt tatctgcatc cgctttcttc tgaaagacgg gggcaagttt gccactttgc
     1861 agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg gttttgtaca
     1921 ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa gggtttgccg
     1981 tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc taaagcaaca
     2041 gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt atatgatgtg
     2101 taatgttata ttgtaacata tgacaaaatg caacaactct taggagatga gagcatagtt
     2161 ttctcaggtg tacttatcta tcttattgaa ataaaatgaa ttatgttgat aatttgaggt
     2221 tgatgacaaa cgatacataa cctgatgtga tctgcgtagg ccggataggg cgctcgcgcc
     2281 acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag ataatttgtt
     2341 gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcgac ttcttatacc gtaaggctaa
     2401 aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct tcactgacca
     2461 cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca actgttgcca
     2521 gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag caagcaaaat
     2581 aatccagctg ataattccta acgttaaata aggccgggta attaactcct tgcctaataa
     2641 cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca gtgtcgccca
     2701 ggcaaagcac cataagccta acaggcggcg tgtgcgtatc aataacggct gttttgcgta
     2761 gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccggt
     2821 aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga tcgcccagac
     2881 cagccagaga aacggcaaca atccggcaag atgcaggcaa actttcagcc atgtcacctg
     2941 ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat acagcgatgc
     3001 cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat cgaggatgcc
     3061 gcctgaacca ataaatcgtt cggtagcctg tgaccagcgt gggtgatcaa catgcggatt
     3121 aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg
     3181 ctcgcgggtc agcttaatac tgacgatcga tttaatccct ttaaagccat atttccacgg
     3241 cacaatcagt cgcaccggcg cgccattttg tggcggtaac gccttgccat aaacacctac
     3301 ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata
     3361 tttcagcccg ccgccgataa agcggtcctg ctggcctggc atctgttccg gtgcataaat
     3421 tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg
     3481 cagcggaaaa ccaatccacg gcaccaccat cgaccacgct tccacgcagc gcatacgata
     3541 aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg tcaatggttt
     3601 tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac cagcattagc
     3661 ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg agactttatc
     3721 tgctggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcgcttt
     3781 tccggcgggg gcgggtgggc gatcgttccc tttaaaccag ctaagcagat cggcatgcgc
     3841 agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca cctgccgacg
     3901 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
     3961 cataagccat cacacttttt gtcgttagcc agcaagagcc gtttactgtt aatctgtcag
     4021 ggagtgtcct ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca
     4081 cgataggttg aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact
     4141 ttattaatat gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg
     4201 aaatagtccc cggttttaaa tacgacacgg taatcgcccg tagttgcagt ttgctcgggc
     4261 cacagtgcct taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg
     4321 ttgtccgctt tcttttcaag agtgactgtc acgtcggcag cgggttttcc tgtttgctgg
     4381 ttcaaaatgt gcacgctaag aatgttttgt tgtgcagcat agaccaaaga aggtaatgaa
     4441 aatgcagccg ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca
     4501 tgtgaatgag ttttgaggat agcgaaaaag cgtccagggt aaccttacag cagcattaca
     4561 attttgtaat gaaccaggct gtttctatga catatgattt atggcatatt attttcatga
     4621 agattttact tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg
     4681 aagcgggtta tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctga
     4741 aggatgatta tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga
     4801 tcttacaaac gttaagaacg gcaaagcaaa cccctgttat ttgccttact gcaagggatt
     4861 ctgtcgatga cagagtcaga gggctggaca gtggagcaaa tgattatctg gtaaaacctt
     4921 tttcattttc tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctttga
     4981 attcaacatt agaaatcagc ggcttaagaa tggactctgt tagtcaaagt gtgagcaggg
     5041 acaatatcag tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca
     5101 gagctggcga aattataccc agaacggtta ttgcgagtga aatttgggga atcaactttg
     5161 atagtgatac caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc
     5221 cttttcctga aaagctaatt gccacaatcc gtgggatggg atattcgttc gtagcggtaa
     5281 aaaaataatg aaaagattat ctataaccgt ccgtttaacc ttgcttttta tattgctgct
     5341 gtctgttgct ggcgccggaa ttgtctggac tctctataat ggcctcgcaa gtgagttgaa
     5401 atggcgcgat gatacaacac tcattaaccg gacagcgcag atcaagcagt tattaattga
     5461 tggggtaaat ccagatacgt tacctgtata ctttaaccgg atgatggatg ttagtcagga
     5521 tatcttgatc attcatggtg atggcatcaa taaaattgtt aaccggacaa atgtcagtga
     5581 tgacatgtta aataacatac ctgctagtga gacaatcagc gcagctggca tttacagaag
     5641 cattattaat gatacagaga tagatgcttt acgaattaat attgatgaag tttcgccatc
     5701 attaacggtt actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa
     5761 aatcaatagc attataattt gcattgtcgc cattatactg tgctcagtat taagtccgct
     5821 gttaatccga acggggttac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa
     5881 ttataacgat agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct
     5941 tgggcaggcg ttgaataaaa tgcatcatgc tttagtcaaa gattttgagc gtctaagtca
     6001 gtttgctgac gatctcgctc atgaacttag aacgccaatt aatgcattac tgggtcagaa
     6061 tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa
     6121 cattgaagag ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgccagggc
     6181 agataaaaac aatgttttgg tgaaactgga ctcgctttct ctcaataagg aagtcgaaaa
     6241 tttgttggac tatcttgaat atctttcaga cgagaaagag atttgcttta aggtcgagtg
     6301 caatcagcaa atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt
     6361 taacgctatt cgatattcgc cagaaaaatc gcgtattcat ataaccagtt ttcttgatac
     6421 caacagctat cttaatattg atatcgccag ccctggagcg aaaattaatg agcctgaaaa
     6481 actcttccgt agattttggc ggggagataa ttcgcgtcat tccgtaggtc agggacttgg
     6541 tctttcttta gtcaaagcga ttgccgaatt gcatggggga agtgctacgt atcactatct
     6601 caataagcat aatgtgttcc ggattacgtt gccgcaaaga aattaacata gggcttcagt
     6661 acgccataaa ttaaccgtga aaaaacgcag ccacgtcgtg agtactaacg cggctgcgat
     6721 tgattatgcg cttacattca aacgtaacag ggattaaccc gcgtaagctg ccagcatttc
     6781 ctgcgccgcc agtttaccca acgcattcgc tgcaaaagga ctgtcgccgg tgagaagttt
     6841 acggtcctta tgtactcgcc cggtgatgtc gtcattaatg atgttcatgc ccattttctt
     6901 cagttcttcg ccgaagtacc aggtgagatg acccggcata tagccaatct ctggcgtttg
     6961 tttgtctgcg gcgtctggga atgcgcaaat ggaataacca ttcagtggat tatcgccgtg
     7021 gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag ataacaaaac ggtcattttt
     7081 gattgcccac tgtaaagcgg cagctacgtc ctggctttca ggtaaaccaa taagtgcgcc
     7141 atgaccacca ggaacaaaga ttgctgcata ttcgctatca gcgttgaggc tggcgacaac
     7201 atccgcgagt ttcttcggat tgcggaacaa cgatttatgc tgctcaaaga atggcattac
     7261 tttttcatct ttctgcggca tagcccagta ttcaaacttg gtcatcagac cggaaatggt
     7321 cgccacttcg aattcgaaac ctgcagcatg gagatgatac aacggcagca acgtttcaat
     7381 cggatggtta ccggtcgaga acagttttcc gttatcggtc ggcaaataac gttcgtccgc
     7441 ggcgatcacc agaattttat gtttaccgcg ataaggtttt ggatagtcca cgccatcaag
     7501 atcagagaca ggactggtat attggctaag cgaatattct gaagggaaga atgcattatc
     7561 ttcagcaata tcgacctgcg gatttttact ggtttgaaca gtcatagtgt attccttatt
     7621 gttgcttagt tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg
     7681 ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg ggtgtaagta tgtaatgatg
     7741 atgctgcact ggtgttgaat attattcacg ccaaattttc cttattgagc aacttcgtcg
     7801 caagtggcct caatgatttt agaccattat cagaggtacg gggcggcggg gagagattta
     7861 gtgctgtgga atattgtggt gctgatgaat atttatatcg cggattggta gagaaaaagc
     7921 ttatttcatg cactattttt gcaaatattt ctatatggga agcgtgtttg ttacggatta
     7981 attcataaag aaatgcattt gggtgttggc ttgaagagtc gctggttgat gttattgtgc
     8041 caggaggggc agggtaaggg ggtgtatcga taagctttgg caattgcgtg tgccgcagat
     8101 ggagatgaaa tgccaataaa aaagcctgag ttcgatgagg gacgaactca ggctagtgtt
     8161 cagttttaca ataacacgcc gcggcgcgtt ggctgattag aactggtaga ctaagcccac
     8221 agccacgata tcatcggtag cgatgccgtt ttctttgtag aagtcgtcgt cgttgtccag
     8281 caggttgatt ttgtaatcaa cataagtgga catgtttttg ttgaagtagt aggtcatacc
     8341 aatatcaaca tatttaacca gatctttgtt ggtgtaatga tgattaccct ggctgtcgaa
     8401 gttatcacta tttacaccgc ctaaatcctt acctttagat tgcagataag cgatggacgg
     8461 gcgcagaccg aagtcgaact gatactgagc aacaacttca aagttttggg ttttgttagc
     8521 gatggcatca gcatcaccgt aaccagtcat gttacggggt tcagcataca tagctgctaa
     8581 gtaaacgtta ttagcgtcgt atttagcacc aacggtccat gcttctgctg tttcaccgcc
     8641 agcagttgca tcgaagcgat ctccataacg agtgttatga gtacctttgt gaacctgatt
     8701 gtcggtacgg tcagagttag agtaagcagc gcccagactt aaaccaaagt caaagtcata
     8761 ggaagtggac atactgaacc gtcgccgttc tctttagcaa gtttacgtcc attaccgtta
     8821 cctgaacctt cttgatcaaa taatccgctt ttctcgttgt taccctgata ctggagtgca
     8881 aagttcagac catcaacttg accaaagaaa ccattgttac gataggttgc tacgctgtta
     8941 gcacgaccgt tcatgaagtt gtcagcgcca gcataggtat ctccaccaaa ttctggcagc
     9001 atatcggtcc atgcttctac gtcatatacg acaccgtagt tacgaccata gtcgaaagta
     9061 ccattctgac caaagccgaa acctgcaaat gccagacgcg ttgcggtatt acccttgtcg
     9121 ctttcaggac cgtttgcgcc aatgttaaat tcccactgac cataaccagt gaattggtca
     9181 gcgatctgag tttcaccttt gaaaccgata cgggcatatg acatgtcgcc atcgctgcta
     9241 gcatcatcag agaagtagtg caggcccgct acttttccgt acaaatccag tttattgcca
     9301 tctttattat aaatttcagc cgcatttgct gcgccagcaa ctaataacgc cgggaccagc
     9361 attgccagaa cttttctttt cattatgtat tcccttgtga ttataatctt catgaatata
     9421 tcaataagtg ccgttatcca
//
LOCUS       DAZFKY010000179         9141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFKY010000179 DAZFKY010000000
VERSION     DAZFKY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            59..421
                     /locus_tag="ACHKCL_003893"
     CDS             59..421
                     /locus_tag="ACHKCL_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764768.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(600..1058)
                     /gene="ybbJ"
                     /locus_tag="ACHKCL_003894"
     CDS             complement(600..1058)
                     /gene="ybbJ"
                     /locus_tag="ACHKCL_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5764769.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1055..1972)
                     /gene="qmcA"
                     /locus_tag="ACHKCL_003895"
     CDS             complement(1055..1972)
                     /gene="qmcA"
                     /locus_tag="ACHKCL_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5764770.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2118..2795
                     /gene="fetA"
                     /locus_tag="ACHKCL_003896"
     CDS             2118..2795
                     /gene="fetA"
                     /locus_tag="ACHKCL_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5764771.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2782..3561
                     /gene="fetB"
                     /locus_tag="ACHKCL_003897"
     CDS             2782..3561
                     /gene="fetB"
                     /locus_tag="ACHKCL_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5764772.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3624..4478)
                     /gene="cnoX"
                     /locus_tag="ACHKCL_003898"
     CDS             complement(3624..4478)
                     /gene="cnoX"
                     /locus_tag="ACHKCL_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5764773.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4539..5348)
                     /gene="ybbO"
                     /locus_tag="ACHKCL_003899"
     CDS             complement(4539..5348)
                     /gene="ybbO"
                     /locus_tag="ACHKCL_003899"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5764774.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5338..5961)
                     /gene="tesA"
                     /locus_tag="ACHKCL_003900"
     CDS             complement(5338..5961)
                     /gene="tesA"
                     /locus_tag="ACHKCL_003900"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5764775.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5932..6618
                     /gene="ybbA"
                     /locus_tag="ACHKCL_003901"
     CDS             5932..6618
                     /gene="ybbA"
                     /locus_tag="ACHKCL_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5764776.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6615..9029
                     /gene="ybbP"
                     /locus_tag="ACHKCL_003902"
     CDS             6615..9029
                     /gene="ybbP"
                     /locus_tag="ACHKCL_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5764777.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2071 a         2361 c         2520 g         2189 t
ORIGIN      
        1 tgcgtttctt tgataaatct caatttttaa gaggtttacg ccaggcagga aaatttaaat
       61 gaatgaattt gagaagattt ttaatgaaat gaatctggac cgggcattgc taccgatcct
      121 ttttcgttcg aaccggtcga cagtgtggaa gtatttaagc ggcgatagta cggctcctgc
      181 ctctgccatg tcgctgatta tgctgttgca gttgatacag aaacgaaacc ccgatctgtt
      241 agccgaatgg ttaacgcttt ctgatttcac cattccgcca gaagtttacc tggaccagcc
      301 tgattactgg aaagggtggg tgtatacgca acacaaggtc aataaaaacg tcctggaata
      361 tctaaaaaaa cactatccgg atgaagacca aaaaagtatg agtaaaggcc gggaagagta
      421 aattgccaat gccagttaac atttaacatg gcggaggcgg cctacggttt tggcgtgata
      481 tgacgcgccc gcgtcgcatc aggcgtcggt gcacgactgc cggatgcggc gtaaacgcct
      541 tatccggcct acgattcggg cgcagacttc cgggcatgat aagacgcgtc agcgtcgcat
      601 caggcgatga ccgcacggat gatcagcgtt atcccttcta tcgcaatgac ttcaacatgc
      661 gtacctgcgc cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
      721 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
      781 ccgcgctggt ttaaatgact gtcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
      841 ttccaccaca gccaggcggc gagcagcgtc aggacggcaa acatcacccc ttgccactcc
      901 caacccagcg gcaccagcca gaccaccagg ccagtaatca ctgctgccac gccactccac
      961 aacaaataac catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc
     1021 cagaaaatat gtggatgaac gactattaac tccatcatgg ctgagtccgc ttgttggcgc
     1081 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
     1141 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
     1201 tctgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatggct tcagacacca
     1261 ttttggtggc gcgggcttct gcttcagcgg aacgttcacg cgcttcagcc tgtaaaaacg
     1321 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
     1381 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
     1441 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
     1501 caatacgggt gactttaatc ccccacgggt tggtggcctc atcgacaata cgcagcaggc
     1561 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
     1621 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag
     1681 ccgcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
     1741 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
     1801 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
     1861 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
     1921 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
     1981 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
     2041 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
     2101 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
     2161 atctggcggg tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
     2221 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
     2281 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
     2341 agccagaaat ctaccgtcag caagtctctt actgcgccca gacgccgacg ctgtttggcg
     2401 atacggtata cgataatctg atctttccct ggcagatccg taaccagcag cctgacccgg
     2461 ccatttttct cgattttctt gaacgcttcg ccttgccgga cagcattttg acgaagaata
     2521 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
     2581 tgccgaaggt tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata
     2641 acgttaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
     2701 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
     2761 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
     2821 gcattaatgc tggtagtggt ggcaatctta attagccata aagaaaaact ggcgctggag
     2881 aaagatattc tctggagcgt cgggcgcgcg ataattcagc tgattattgt tggctatgtg
     2941 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
     3001 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
     3061 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
     3121 gggtcgattg aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac
     3181 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
     3241 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
     3301 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
     3361 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
     3421 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
     3481 ataattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
     3541 acgcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
     3601 ttcatgtgcc gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
     3661 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
     3721 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
     3781 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
     3841 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc
     3901 agcagttcga tttgcgccac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
     3961 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
     4021 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
     4081 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
     4141 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
     4201 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
     4261 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
     4321 ttgtactgcg ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt
     4381 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
     4441 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
     4501 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
     4561 atcacgcgcc ccggcagcag gcgcttaagc accattaccg cccaggtcac cagcgttacc
     4621 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct
     4681 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
     4741 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
     4801 ttaattccgc tgcggcgcag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
     4861 ttactggccg cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgaggtc
     4921 atcacaatac gcccttcacc gtgcggcaac atcgcgggta acaggcgcat ggtgagctgg
     4981 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
     5041 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
     5101 agggcgatca cctcgtcggc tgcgcgatca acactttctg gtgaatccag atcgatcaac
     5161 acgccggtaa atcccatgct gttcatgcgc tcaacatcat ccggtttccg gcaacctgcc
     5221 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
     5281 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
     5341 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
     5401 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
     5461 ttccattaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
     5521 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
     5581 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
     5641 ctgtggctga aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg
     5701 ctgatgctgt ttcagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
     5761 gatgctggca ttgactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
     5821 ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt
     5881 gtccgctgcg gcagcacgga aggttaacag gaccagaaac aggaagggca aatgccagcg
     5941 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
     6001 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
     6061 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggctgga tgacggcagc
     6121 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
     6181 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
     6241 aacgcgctgg aaaacgtcga gcttccggcg ctgctgcgcg gtgagagtag cgcggaaagt
     6301 cgtaacggag cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
     6361 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaacggt
     6421 cgacctgatg tgctgtttgc cgacgaaccc accggtaacc ttgaccgcca gacgggcgat
     6481 aaaattgccg acctgctgtt ttccctcaac cgtgagcatg gcaccacgtt gattatggtg
     6541 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
     6601 ttgcaggagg aagcatgatt gcacgctggt tctggcgcga atggcgctcg ccgtcgctat
     6661 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
     6721 gcgatcgcat ggagaagggc ttaagccaac aaagccggga gtttatggcg ggcgatcggg
     6781 cgttgcgcag ttcgcgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
     6841 tgaaagtcgg caagcagctg actttcgcca ccatgacatt tgcaggcgac acaccgcagc
     6901 tggcgaacgt taaagcggtg gatgatatct acccgatgta tggcgatctg caaaccaatc
     6961 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
     7021 ttaacctgaa aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag
     7081 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
     7141 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
     7201 gctataaatt cggcggcagc gagaaccagc tcgacagcta tgagaaatgg ttgttacctc
     7261 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
     7321 cgatggaacg ctcgcaacag ttcctgctgc tttcggcact tctgaccttg ctgctggcag
     7381 tggcagcagt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
     7441 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
     7501 tgatggtgct gacgctttca gccgttaccg gtggggcgat aggcctgttg ttcgaaaacg
     7561 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
     7621 catggctatg ggcgctgggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
     7681 accgcttgtt gctggcaacg cagcctttac gcgtattacg taattatgtg gttgcgaacg
     7741 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
     7801 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
     7861 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
     7921 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
     7981 agctttcggc attttcgctc tcctttatgc tgctggcact gctactggtg ctgcgtggcg
     8041 atctgctcga ccgctggcag cagcagttac cgccagaaag cccgaactac tttttaatca
     8101 acatcgccac agaacaggtt acgccgctaa aagcgttcct cgcggaacat cagatagtcc
     8161 cggaatcgtt ttatccggtg gtgcgagcgc ggctgacggc gattaacggc aagccgacag
     8221 aaggcaatga agatgaggcg ctcaaccgcg aactcaatct tacctggcaa aatacgcggc
     8281 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agctgatgaa gtttcgatgg
     8341 aagaggggct ggcgaaacgc ttaaacgtta ccctcggcga taccgtgact tttatgggcg
     8401 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
     8461 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
     8521 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
     8581 ctaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
     8641 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
     8701 tgctggcaca ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca
     8761 cactcggtgc ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg
     8821 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
     8881 cgaaagtgtt tgattttccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
     8941 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg cgcgagactg gttaagggta
     9001 aggcgctgtt caggcagttt gcggggtgat gagagtaaat aaggggcgtt ctggttgaat
     9061 ggaacgcctt gtttaaatta attctacaat atgtttcatt gatgatgtta ttgaattggt
     9121 gatttcctat ctttctattg c
//
LOCUS       DAZFKY010000180         9022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFKY010000180 DAZFKY010000000
VERSION     DAZFKY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(6..908)
                     /gene="yihR"
                     /locus_tag="ACHKCL_003903"
     CDS             complement(6..908)
                     /gene="yihR"
                     /locus_tag="ACHKCL_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO5764778.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(975..2216)
                     /gene="yihS"
                     /locus_tag="ACHKCL_003904"
     CDS             complement(975..2216)
                     /gene="yihS"
                     /locus_tag="ACHKCL_003904"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO5764779.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(2232..3110)
                     /gene="yihT"
                     /locus_tag="ACHKCL_003905"
     CDS             complement(2232..3110)
                     /gene="yihT"
                     /locus_tag="ACHKCL_003905"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO5764780.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3134..4030)
                     /gene="yihU"
                     /locus_tag="ACHKCL_003906"
     CDS             complement(3134..4030)
                     /gene="yihU"
                     /locus_tag="ACHKCL_003906"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5764781.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4198..5094
                     /gene="yihV"
                     /locus_tag="ACHKCL_003907"
     CDS             4198..5094
                     /gene="yihV"
                     /locus_tag="ACHKCL_003907"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO5764782.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5128..5913
                     /gene="csqR"
                     /locus_tag="ACHKCL_003908"
     CDS             5128..5913
                     /gene="csqR"
                     /locus_tag="ACHKCL_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5764783.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6012..6611
                     /gene="yihX"
                     /locus_tag="ACHKCL_003909"
     CDS             6012..6611
                     /gene="yihX"
                     /locus_tag="ACHKCL_003909"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5764784.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6605..7477
                     /gene="yihY"
                     /locus_tag="ACHKCL_003910"
     CDS             6605..7477
                     /gene="yihY"
                     /locus_tag="ACHKCL_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5764785.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7474..7911
                     /gene="dtd"
                     /locus_tag="ACHKCL_003911"
     CDS             7474..7911
                     /gene="dtd"
                     /locus_tag="ACHKCL_003911"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5764786.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            7956..8897
                     /gene="fabY"
                     /locus_tag="ACHKCL_003912"
                     /gene_synonym="yiiD"
     CDS             7956..8897
                     /gene="fabY"
                     /locus_tag="ACHKCL_003912"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5764787.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2089 a         2277 c         2435 g         2221 t
ORIGIN      
        1 taaaattaat tgtgttgccc gtaaatatta aaaaacaggc ggtgcgtttt ccctggttcc
       61 agtaataata agtcgagacc tgaattaaag gcgttaggcg ggcaactcat aggttcaact
      121 gccagtccct gacgctgtaa tttttcaccg ctataaattt gtacccaggg ttgatcggaa
      181 caaaggctaa ccgaaagcgc ctgttgtgga tgggtaatgg ttatttccca cagatcatta
      241 gcggttttaa aggtgtggtc gatttttgtc gcggctattt ttttcgcctg cgtgaaattt
      301 aaatccagct catcaacgtg gtgaagcgtt gtcggattag cgtgctcatc gacggcataa
      361 acctggtttg ccggaagctg gaacagatat tcatcaacag atgttaagtt gcaggttaaa
      421 taaggatgaa tacccacacc atagggtgcg gtcacagtac caatattctg acttgcgatc
      481 tcaacggaaa gacccgtgcg tgcattgagc gaataaacaa cctgcgaagc cagcataaac
      541 ggatagccgt aactgggcgg cagaaaagct gtcagggtaa cgctggtggc ggttaattcg
      601 ctgatttgcc agtcacgcca ggcgagcagc ccatgaatag cggcttttga accatgttca
      661 ttaatgggta actgatattc ctgcccctga tagcgataac agccgttggc gatccggttc
      721 ggccacggaa taaggacttt gcccagatgt gccagcggca tctcttcggg tttatgtgga
      781 ataactaaat gacagccctg aaaggttaat tcagccagac ctgcaccgac ggtcacaatg
      841 gtcgcgtgat aatcccccgc ggcaagtgaa acggtctgtc cactgcaatg catgttggtg
      901 atttgcatac gaggtacctt tattgaataa cgacataccc ggcgtgcagc ctgatatgtc
      961 gtttttttag tcagttattt cgcgttaata tccagcagac ccgccgcaac tgccggagcc
     1021 aggcctggcg ctaacgggat acgcgggatc accaggcaat gcagcaggtg ataaatatcc
     1081 tgtttgccgt cccagacttt ggtggtgacc ttattgtccg catccagctc ctgccaccat
     1141 gaaccatttt cgtagtccat caggtatttg atgcagtagt cccaccatgt ctgataccag
     1201 gtttcgtact ggcgatcgcc ggtgacggtg tagagcgcgt aggccgtacc catcgcttcg
     1261 acgataggcc aacgcacacg ttcgcggacc accggttttc cttcccagtc aacggtataa
     1321 acaatcccgt ccgcaccatc gggtgcccag gcatcacgca cggtggcgtt aaacagacct
     1381 ttggcatctt ctagcagcca tgctggcggt tgttcgcaac gggcttccag ggccgcgtgg
     1441 atgtgcagca ttaaacggcc ccattcgatc cagtggcctg gtgtacctcc gaacgcgcgg
     1501 aagcggtgcg ccgggttatc tttgttgtaa tccggcagcg gattccactg ggtatcgaag
     1561 tgttcgttaa cgcgataatg attatttctt gcgacgtcgt ggataatcac ggaagccacg
     1621 cgaatcgcgc gatccagcca ttttttgtca tgcgttacgt cataaacaat taagaaagct
     1681 tccactgcgt gcatattggc attgccgccg cggtactctt cggttttgct gaaggcttcg
     1741 tcccaggact ccaggcacat ctgctcttct tcgctccaga agtatttctc gataatttca
     1801 atggtgtaat cgagcagctt gcgcgcttcc gggtgacccg ttgtgacggc gctggcagca
     1861 cccagcagag caaagaaatg ttgatagccc tgtttggagg catccaccac gccctcgtca
     1921 ttcacacagg cataccagcc gccatatttt ttatcgcgca gtgcgccgtt catggcttta
     1981 ataccgtgat caaccaacga gtaagcgcca ggtcgaccca tcgccgcagc aacggaataa
     2041 acgtgcaaca tacgagctgt gatccacaga tgggtgccca tctcttcttt gatttgccct
     2101 ttattgccta accagccaaa tccggtcggt actacggaat ttttaccaaa atcaaagatg
     2161 cgatcggttt cctgttccag ccaacggttg tgacttaaag tgttaaacca tttcattttt
     2221 gtatccccat tttaacggcg tttagccatc atttcgtcga cgatatcgcc aagttgttgt
     2281 aatttcggtg cgcaaacgtc acgcagcatc agctcgttgt ctggtaaacc gacgaccgat
     2341 gcccagacag cacgaccagc caggaagccc gatgcacccg ctgtcattgc cacgcgtacg
     2401 gcacgcggga acagtttttc gtcgacaccg gaagagagga tcacccacgg catattgata
     2461 tggtcattca gacgttgtga agcactgaga agctcttgtt gcggaccttt accgtaaagc
     2521 ggcatttcaa ctttgtagag gtcagcgcca ctgtcgccca gctctttggc ggcatcgatg
     2581 atcgcttgtt cgcgatcgaa tttatcgcca cgacgtggtg ggcggacgac tggctcaata
     2641 atgcttacca ggccgtgtga gtggcacagt tcgttaaact ctttcaccat atccaggcgt
     2701 tgctgcgcat cttcatcact acgccacagc accagcagtt ttaaggcttt gccaccgtct
     2761 tgtttgatct gtagcggatt gattttgcgg tcaatgacca cgctatctac cggaataccg
     2821 ttgccaggaa tgaactcatc ggcggcgaca atcatggcgc aacttttggc aatcgcgttt
     2881 tgctcaacca cctggcggta acagaattgt tgatctacca gaatcgccga ggcataaggc
     2941 gagagggtct tcgccgcgtt aactttgaaa tcagttaaaa cgctatcggc taccggagcg
     3001 ggtgccccag ccgcggcaaa catcatgcgc atggcttcgc gctgatcgac cgccagcatg
     3061 gcaaaaccgc ccgatgcgcg cgtaatgtcg ttgatggtgt acttattcat tcatttattc
     3121 ctttatccag tcgttacatt tttactttgg cagtcatccc ggcactgaca cggacctgtt
     3181 ccagaatggc ggaccagtcc tggcgaccgc gacccgctgc gcgcgcctgg ctataaacct
     3241 cccgtgaggc ggcccccagc ggcattggca catgcagctg gttggcgaca tcaagggcga
     3301 tgccaagatc cttatgggca agatcgatca tgaaggcggg agaaagatcc ccgctgagga
     3361 ctttgttcgg ccaggaagtg gtgaagtggc ctttaccggc ggcggtaccg ctcatcactt
     3421 tgacggcaac atcgaaggga agattcaggg cttcgcacaa aacggcagct tctgccgaaa
     3481 gcgcattgag cgcgatgctc atatagttgt tgatgagctt aacgcggatc cccatgcccg
     3541 gaccgcctgc gttgatcaac tcactgccca tcgccatcag gatcggcgtg gcacgttcaa
     3601 cttgttcagc ggtgccgcca gccagcagta acagagtacc ggtaatggca tttgcagaag
     3661 tacggcctac cggaacatcc atcatgttga agcctttggc ttgcatatcg gcaatcaatt
     3721 tatcggtttg cagcggatgg atggtcgaca tatcaatgac cagcgcatcg gtagataagc
     3781 tttcgcaaac gccgttttca ccgaacaaca cgctgcgcac cagatcgcca ttcggcaaca
     3841 tggtaatgat aaattcggca tctttagcgg cctgcgccgg gttggcggcg ggagtcgcgc
     3901 ctttgtctac cagatgccgc acagcctcgg cattcacatc aaagacgcga agttggtgcc
     3961 cttgctgcaa taaattgctc gccattggcg aacccatttg tcctaaaccg ataaacgcga
     4021 ttgctgccat aaccttctcc tgaatacagt gatgtcactt tttgtcattt atgacacgct
     4081 ttgattgtct gtttttgatc gtatttgtaa tttatagtca aaaaattgac agacgtcact
     4141 ttttaaacaa ttggtgaaat taaaatgaac gcatcccaaa atgtttaagg aatgaccatg
     4201 attcgtgttg cttgtgtagg tataaccgtg atggatcgca tctattacgt ggaagggtta
     4261 ccgacggaga gcggtaaata cgtggcgaga aattatacgg aagttggtgg cgggccagcg
     4321 gcgacggcag cggttgcggc ggcaagactg ggagcgcagg ttgattttat tgggcgcgtc
     4381 ggtgatgacg acaccggcaa cagcctgctg gcagagctgg aatcctgggg cgttaacacc
     4441 cgctacacca aacggtataa acaggcgaaa tcgtcgcaat ccgccatcat ggtggatgcc
     4501 aaaggcgagc ggataatcat taactacccc agcccggacc tgctgcctga cgcagagtgg
     4561 ttggaggaaa ttgatttctc tcagtgggat gttgtgctgg cagatgtacg ctggcacgac
     4621 ggcgctaaaa aagccttcac tctggcccgg caggcgggcg tgatgaccgt tctggacggt
     4681 gatattacgc cgcaggatat cagcgagctg gtggcattaa gcgatcatgc ggccttttca
     4741 gaaccgggtc tggcgcgctt aacgggcgtg aaagagatgg ccagtgcgct aaaacaggca
     4801 caaacgctca caaatggtca tgtctatgtg acccagggca gcgcaggctg tgactggctg
     4861 gaaaatggcg ggcgtcaaca tcaaccggcc ttcaaagttg atgtggtaga taccacaggt
     4921 gcgggtgatg tttttcacgg cgcattggcg gtggtgctgg caacaggtgg ggatttagcg
     4981 gagtcagtcc gcttcgccag cggtgtagcg gcgtcgaaat gcacgcgtcc cggtggacga
     5041 gccgggatcc ctgactgtga tcaaacccga tcttttttgt cactttttgt ataaaatgcc
     5101 agggtgatgg tttttcgagg aattttcatg agccttaccg aactaaccgg taacccgcgg
     5161 cacgaccaac tcctcatgct gatcgccgag cgtgggtata tgaatattga tgagctggca
     5221 aatctgctgg atgtctccac gcagacggtg cgccgggata ttcgtaaatt aagcgagcaa
     5281 ggcctgatta cgcgccatca cggtggcgcg ggtcgggctt ccagcgtcgt taatacggcg
     5341 ttcgagcagc gtgaggtttc gcaaaccgag gaaaaaaaag cgattgccga agcggtggca
     5401 gactatattc ctgatggttc aacaatattt atcaccattg gtacgaccgt tgagcatgtt
     5461 gcccgggcgt tacttaacca taatcatttg cggataatca ccaacagcct gcgtgtggcg
     5521 catattcttt accacaaccc gcgctttgaa gtgatggtac ccggcggtac gttgcgctct
     5581 cataatagcg ggatcattgg cccttcagcg gcgtcctttg tggccgattt tcgtgctgat
     5641 tatctggtaa caagcgttgg ggcgattgag agcgatggcg cgttgatgga gtttgatgta
     5701 aacgaagcta acgtggtgaa aacgatgatg gcgcacgcga gaaatatcct gctggttgcc
     5761 gatcacacta agtatcatgc ttcggcggcg gttgaaattg gtaacgtggc acaggtcacc
     5821 gcgctcttta ccgacgagct gccgcccgct gcgctaaaat cacgcttaca agacagccaa
     5881 attgaaatca tccttcccca ggaagacgcg tagatttttg tgactgttga cctggcttca
     5941 taccttgcca catagccaaa cccatccttt cccgctacag ttaatttctt gtggcgcgaa
     6001 aggaggcaaa aatgctctat atctttgatt taggtaatgt gattgtcgat atcgacttta
     6061 accgtgtgct gggaacctgg agcgatttaa cgcgtattcc gctggctacg cttaagaaga
     6121 gttttcatat gggggaggcg tttcatcagc atgagcgtgg ggaaattagc gacgaagcgt
     6181 tcgcagaggc gctgtgtcat gagatggctc taccgctaag ctacgagcag ttctctcacg
     6241 gctggcaggc ggtgtttgtt gcgctgcgcc cggaagtgat cgccatcatg cataaactgc
     6301 gtgagcaggg gcatcgcgtg gtggtgcttt ccaatactaa ccgcctgcat accaccttct
     6361 ggccggaaga atacccggaa attcgtgatg ctgctgacca tatctatctg tcgcaagatc
     6421 tggggatgcg caaacctgaa gctcgaattt accagcatgt tttgcaggcg gaaggttttt
     6481 cacccagcga tacggtcttt ttcgacgata acgccgataa tatagaagga gctaatcagc
     6541 tgggcattac cagtattctg gtgaaagata aaaccaccat cccggactat ttcgcgaagg
     6601 tgttatgcta aaaaccattc aggacaaagc caggcaccgt acccgtccac tatgggcctg
     6661 gctaaaacta ctctggcaac gcattgatga ggacaatatg acaaccctgg caggtaacct
     6721 tgcctatgtg tcgttgctct cattagtgcc gctggttgcc gttgtttttg cgcttttcgc
     6781 cgcttttccc atgttttccg acgtcagcat tcagttacgt cactttattt ttgccaactt
     6841 tctgcctgct actggcgatg ttattcagcg gtatatcgaa caatttgttg ccaattccaa
     6901 caagatgacc gccgttgggg cgtgcgggct gatcgtcacc gcgttattgt tgatgtactc
     6961 catcgatagc gcgttgaata ccatctggcg cagtaaacga gcgcgaccca aaatttactc
     7021 gtttgccgtg tactggatga ttttaacgct ggggccgctg ctggcagggg ccagtctggc
     7081 gatcagttcc tatttgctct ccctgcgctg ggcgagcgat ctcaatactg tcatcgacaa
     7141 cgtgctgcgt atttttccgt tgctgttgtc gtggatctcc ttctggttgc tgtacagcat
     7201 tgttcctacc atccgcgtac ctaaccgcga cgcgattgtc ggcgcgtttg ttgctgcact
     7261 cctgttcgaa gcaggaaaga aaggtttcgc gctttatatc accatgttcc cgtcatatca
     7321 gctcatttac ggtgtgctgg cggtgatccc cattctcttt gtttgggtct actggacatg
     7381 gtgtatcgtc ttgcttggcg cggaaattac tgtcactctc ggggaatacc gcaaactaaa
     7441 acaagcagct gaacaagaag aagacgacga accatgattg cattaattca acgcgtaacc
     7501 cgtgccagcg tcaccgtgga gggagaagtg acgggcgaaa ttggtgcggg acttttggtg
     7561 ttattgggtg tcgaaaagga tgacgacgaa cagaaagcaa accgtctgtg cgagcgtgtg
     7621 ctcggctacc gcatctttag cgatgccgaa ggcaagatga atctcaacgt gcaacaggcg
     7681 ggcggcagtg tgctggtggt ttcccagttt accctcgccg cagataccga acgggggatg
     7741 cgcccaagtt tctccaaagg tgcatcaccg gatcgcgcag aggcgttata tgactatttc
     7801 gtcgaacgct gccgtcagca agagatgaac acgcaaacag gacgcttcgc tgcggatatg
     7861 caggtatcgc tggtcaatga tggccccgtg acattctggt tgcaggtatg agccagcttc
     7921 cagggttgtc acgggaaaca agagagagta tcgctatgta tcaccttcgg gttccacaaa
     7981 cagaagaaga attagagcgt tactatcagt ttcgctggga aatgttgcgt aagcccctgc
     8041 atcaaccaaa aggttcggaa cgcgacgcgt gggatgcgat ggcgcatcac cagatggtcg
     8101 tcgacgagca gggtaatctg gtggcggtag gccgactgta tattaatgcc gacaatgaag
     8161 cgtccattcg ctttatggcc gttcatcccg acgtgcagga caaagggtta ggcacgctga
     8221 tggcgatgac cctggagtcg gtggcgcgtc aggaaggcgt taagcgcgtg acctgtagcg
     8281 cccgtgaaga cgcggtggag tttttcgcca agctggggtt tgttaatcag ggagaaatca
     8341 ccacgccaac caccacgccg attcgccatt ttttgatgat taagcccgtc gccactctgg
     8401 atgatatttt gcatcgcggc gactggtgcg cgcagctgca acaggcgtgg tacgaacata
     8461 tcccgcttag tgaaaaaatg ggcgtgcgca ttcagcaata taccgggcaa aaatttatca
     8521 ctaccatgcc agaaaccggc aatcagaatc cgcaccatac gctgtttgcc gggagtttat
     8581 tctcactggc gacgctcacc ggttgggggc ttatctggct gatgctgcgc gaacgccacc
     8641 tcggcggaac gattattctg gcggatgcgc atatccgcta cagcaaaccg attagcggta
     8701 aaccccatgc ggtagccgac cttggtgcct taagcggcga tctcgaccgt ctggcgcgcg
     8761 gacgaaaagc acgggtgcag atgcaggtcg aaatctttgg cgacgagacg ccgggtgcag
     8821 tgtttgaagg cacgtatatc gttctgcccg cgaagccatt tggcccgtat gaagagggcg
     8881 ggaacgaaga agagtagctg atggagaggg gcggtgctgc ctctctcatt caggtcattt
     8941 atattagtcc ttggctattt cggttatatt gaataagaac tctataaatt tttattcttc
     9001 gttggctcca tacgtgtctt gc
//
LOCUS       DAZFKY010000181         8937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFKY010000181 DAZFKY010000000
VERSION     DAZFKY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(127..2406)
                     /gene="maeB"
                     /locus_tag="ACHKCL_003913"
     CDS             complement(127..2406)
                     /gene="maeB"
                     /locus_tag="ACHKCL_003913"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5764788.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2695..3645
                     /gene="tal"
                     /locus_tag="ACHKCL_003914"
     CDS             2695..3645
                     /gene="tal"
                     /locus_tag="ACHKCL_003914"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5764789.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3665..5668
                     /gene="tkt"
                     /locus_tag="ACHKCL_003915"
     CDS             3665..5668
                     /gene="tkt"
                     /locus_tag="ACHKCL_003915"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5764790.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5763..6806)
                     /locus_tag="ACHKCL_003916"
     CDS             complement(5763..6806)
                     /locus_tag="ACHKCL_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5764791.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6932..7507)
                     /gene="nudK"
                     /locus_tag="ACHKCL_003917"
     CDS             complement(6932..7507)
                     /gene="nudK"
                     /locus_tag="ACHKCL_003917"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5764792.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            complement(7575..>8937)
                     /locus_tag="ACHKCL_003918"
     CDS             complement(7575..>8937)
                     /locus_tag="ACHKCL_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5764793.1"
                     /translation="GFDEIYLPFRADQAQREASRCLKCGEHSVCEWTCPLHNHIPQWI
                     ELVKAGNIDAAVELSHLTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYIS
                     DQALAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLIRNGVAVTVYDRHPEIGGLL
                     TFGIPSFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGVGTYR
                     SMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTAMD
                     CVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHV
                     CGIRFLRTRLGEPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVD
                     KWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGVKSV
                     KSH"
BASE COUNT         2113 a         2439 c         2299 g         2086 t
ORIGIN      
        1 aatatcgaga tattttatat actgcttttt ttattattcg caaactcagt aataagcttt
       61 actaatatat tgcagctaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaaa
      121 aaagaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca tgttgacgat
      181 acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac
      241 cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc
      301 agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat
      361 acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat
      421 cagttctggc gcacgttccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg
      481 gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggtt caataccgaa
      541 acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc
      601 gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcgttcat
      661 tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaaatg
      721 ttcatgataa tcacccaccg taccgcaaat cattgcatcg gcttcgccac gctgaaccat
      781 gatcgcgccg atcactgtcg ggttactgat cagcgcccgc tgcgcctgtt cctgagtgac
      841 gccgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga
      901 ttcgttattg acgatctcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat
      961 gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac
     1021 cagttcctga gtggcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt
     1081 cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa
     1141 ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga
     1201 ctccatcgcc gctttcgcga ccgcaggagc aatcttgact atcagacgcg gatcaaacgg
     1261 ttttggaatg atgtattcag ggccaaagct cagatcctga tcgccatacg ctgaagccac
     1321 cacttcactc tgttccgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat
     1381 ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca
     1441 aaggacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg
     1501 acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat
     1561 cattggcgct cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca
     1621 gccgaggaaa atatctgcgc cttcaatcac atcatcgagg gtacgtttgc cgtcatccac
     1681 taccgcatat gcggctttgg tttccgccat gtttggctca cggccctgat agataacgcc
     1741 ttttgaatcg caaaccacga tgttatgttt ttgcagaccc aacgctacca gcaggttcat
     1801 acaggcgatt gctgcagcac ccgcgccgga aaccaccata cgcacgtcag agatgttttt
     1861 ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg ataattgccg tgccgtgctg
     1921 atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca
     1981 ttctggcgct ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcagcgac
     2041 gacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc
     2101 agcgaatttc ttaaacagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc
     2161 aatgttgcct aaccccagca ccgccgtacc gttagagatc accgccacca ggttaccgcg
     2221 cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac
     2281 gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac
     2341 ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc
     2401 atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc
     2461 tgcccacaac gtggcgcaca ttattacctt gccggagtct acagactttg agcaagtcca
     2521 aactctcacc attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt
     2581 aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca
     2641 gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac
     2701 gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc
     2761 attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc
     2821 ggattatcac aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc
     2881 aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct
     2941 gaaatcctca aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt
     3001 gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc
     3061 gttgagaaat cacgcattct gatcaaactg gcttcgacct gggaaggaat tcgcgcggca
     3121 gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcacag
     3181 gcgcgggcct gtgcagaagc gggcgttttt ctgatttcgc cgtttgtcgg gcgtatttat
     3241 gactggtatc aggcacgcaa gccgatggac ccatatgtgg tggaagaaga tccgggcgtt
     3301 aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg
     3361 ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact
     3421 atcgcaccga atttgctgaa ggagctgcag gaaaaagttt cgccagtggt acgtaaatta
     3481 atcccacctt ctcagacgtt cccacgccca gctcccatga gcgaagcgga gttccgttgg
     3541 gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc
     3601 gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg
     3661 ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca
     3721 aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct
     3781 gtggaacgat tttcttaaac ataaccctac cgacccaacc tggtatgatc gcgaccgctt
     3841 tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctgcatc tgaccggtta
     3901 cgacctgccg ctggaagagc tgaagaactt tcgtcaactg cattcgaaaa cccctggcca
     3961 cccggaaatc ggctatacgc ccggagttga aaccaccacc ggtcctcttg gacaaggttt
     4021 ggcgaacgcc gtcgggctgg cgatagcgga acgtacactg gcggcgcagt ttaaccagcc
     4081 ggatcatgaa attgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga
     4141 aggtatttcc cacgaagtct gttcgctggc gggcacgctg ggactgggca agctgattgg
     4201 tttttacgat cacaacggta tttcgattga tggagaaacc gaaggctggt ttaccgacga
     4261 tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacga
     4321 tccgcaggcg gtgaaggaag caatccttga agcgcaaagc gtgaaagata agccgtcgct
     4381 gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc
     4441 gcacggcgca ccgctggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca
     4501 ccatccgcca tttgagatcc ctaaagatat ttatcacgct tgggatgccc gcgaaaaagg
     4561 cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca
     4621 actggcagaa gagtttaccc gtcggatgag cggtggttta ccgaaggact gggagaaaac
     4681 gactcagaaa tatatcaatg agttgcaggc gaatccggcg aaaatcgcta cccgtaaggc
     4741 ttcgcaaaat acgcttaacg cttacgggcc gatgctaccg gagctgctcg gcggttcggc
     4801 ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc
     4861 ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg
     4921 cattgcgcac cacggcggct ttgtgccgta taccgcaacg ttcctgatgt ttgttgaata
     4981 cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac
     5041 ccacgactca attggtctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc
     5101 cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc
     5161 ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga ttctctccag
     5221 gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg
     5281 ttatgtgctg aaagacagcg gcggtaagcc agatattatt ttgattgcca ccggttcaga
     5341 gatggaaatc accctgcaag cggcggagaa attagcggga gaaggtcgca atgttcgcgt
     5401 ggtttccctg ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt
     5461 gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta
     5521 caagtatgtt ggtctgaaag gggcaattgt cgggatgacg ggttatgggg aatctgctcc
     5581 ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca
     5641 taaggtgctg ggagtaaaag gtgcctgatg gtgatcgccg gatgctgatt gccggatgcg
     5701 acgctgacgc gtcttatccg gcctacagcg tcttatccgg cctacatgtc ccgccatttt
     5761 gtttaacggg tgatccacaa cgtgggccag gcgtctggcc catgccagtt atcgcaggtg
     5821 ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt caaagcgcca gcgcgcctga
     5881 atgccacaat cgcttaatcc gcgccctttc gctaaggtca ccagttcacg cgatttctca
     5941 tcaaatgttg cgttcatcag ttccagttca ttcgtctcct gaccgttgtt gaacggcaaa
     6001 cgcagccgga ccgggcgcga ggccagcggc tttttacgcg acacaatcca tgccaaatca
     6061 atggtgttat aggcccctgc ctcacagcta atcatcatca gcgctttatc atcagtcagc
     6121 gcagtgacat tcacctcacg acgcaatgga tcaagcgagc agcgcagacc attcatccgc
     6181 cagtttccat aatccagcaa atcgttgcgt tcttcgagtg agagtggcgt cggcgttggg
     6241 ttaaccacca cgacctcttt cagcgcaggc gcgggcggta cgctgagcgg cggttcgtcc
     6301 cctttcttga tccacgcggt ttcactgcca acgcgctttt gctgagcatc aataaacaac
     6361 aacgccgctt ttaagtcact cagagaaatg gtctgattgc cgtcgcgtaa ggtgatcgcc
     6421 ttcccttcct gaatcatctg caaaaacgcg gtgatggttg ccgtatcatc ggttactaat
     6481 aaccatggtg aaatccgcca cttgtcacca cttaacgcca acggctcgcc atctaacagc
     6541 agccgtggcg caatttcccc ttcagacgca tccggcgact tcaacccgcc gcgctcaata
     6601 cgtaaaacgg catcggtatg cgccccggcg ctgcggctca gggtcatcac cagtccatta
     6661 tgatcgcccg tattacgcgc cacgcagaag ttttggttat tacaggtgac ctgccagtcg
     6721 gaaaacgccc gttgcgctgg tgccgcccac accaaagacg tcggcaacaa agcgaaaaaa
     6781 aagagaagga aaatgcgata gcgcatggac ggtacgaccc cagaagttca aacaaacagc
     6841 acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc ggatttgtcg gattgagcca
     6901 agaataaaca acgaaatatt attgttattt ttcagtctat taaatgtgac atttgcaaat
     6961 agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc ggttttgatc atctccagcg
     7021 cctggctgaa cggcagctcg agcacttcaa tatcttcatc ttcgacaccg ccaccggcgt
     7081 tagcgcgctg attgtcactg tattcggcga taaaaaagtg gattagctca gtcacaccgc
     7141 ctggcgacat atacagttca aataatttgc gcacttcgcc cacttcatag cccgtctctt
     7201 caatcgcttc tttgcgaatg cacacttccg gttcgtcgtt atccagcagc ccggcgcagg
     7261 tttcaatcag ctgcccgctt tcattgccat taacccaggt agcgacacgg aactgacgaa
     7321 tcagaaccac ggtctttttc ttcgcgttgt acaggaggat cgtcgcacca ttaccgcgat
     7381 cgtaaacttc acgtttatgg cggataactt cgccatcttt gcgcgtgaga tcgtaagtaa
     7441 tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc tttaatgagg gtgatttgtt
     7501 gcgtcatacc gactccacag cgcgaaatga acaattatat tacgctgtga agtcgggttt
     7561 gtctgcgcag gctatcagtg agatttgact gattttaccc ccagccagtc aataatcccc
     7621 tgtgccgcat gacgtccttc tgccattgcg gtaaccacca gatccgcacc acgcacggcg
     7681 tcgccaccgg cgaagatttt cggattggtg gtctggtaac ggtactggct ttccacatcc
     7741 gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg actccagcca cggcatcccg
     7801 tgcggattga agccaaacgc cataatcacc gcgtcggctg gcatgacaaa ttcactgcct
     7861 tccaccggca ctggacgccg acgcccctgg gcatccggct cgccaagacg cgtgcgcagg
     7921 aaacgaatcc cgcagacgtg accttgttca ttcagctcaa gcgccaccgg ctggacgtta
     7981 aattcgaagt tggccccctc ttcgcgggcg ttcttcacct ctttcttcga gcctggcatg
     8041 ttagcttcat cacgacgata agcgcaggtg acgttactcg cgccgtggcg cagcgcagta
     8101 cgcacacagt ccatcgcggt gtcgccgccg cccagtacca cgacgttaag tccggcggta
     8161 ttgataaacg gctcttccgg tagctcttcg agccccatca cctgtttagt gttggcgatg
     8221 aggaacggca gcgcgtcata aacgcccggc gcatcttcat tgggtaaacc cgctttcatg
     8281 gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt gttccaaaag cgaatccaaa
     8341 gagacatctt tacccacttc acaattgagt tcgaagtgaa tccccatcgc gctgaagatt
     8401 tcccgacgac gtgccagcag ggatttatcc agtttgaaag aaggaatgcc gaaagtgagc
     8461 aagccaccga tttctggatg gcgatcgtac accgtcaccg ccacgccatt gcggatcaga
     8521 acatccgcac aggccagccc tgccggacct gcaccgataa tcgccacccg cttgtccact
     8581 ttggtgacat ggcttaagtc aggacgccaa cctttcgcca acgcctgatc tgaaatgtag
     8641 cgttcaatgt tgccgatagt taccgcaccg tgctcatcgc gaatagtaca ggcaccttca
     8701 cacaaacggt cttgcggaca aacgcgtccg gtaatttccg gcagggtgtt ggtcaggtga
     8761 gaaagctcga cggcggcgtc gatgtttccg gctttcacca gttcaatcca ctgcggtata
     8821 tggttatgca gcgggcaggt ccattcacaa acgctgtgct cgccgcattt aaggcagcgc
     8881 gaggcttccc gttgtgcctg atcggcacgg aatggcagat aaatttcatc aaaaccg
//
LOCUS       DAZFKY010000182         8784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFKY010000182 DAZFKY010000000
VERSION     DAZFKY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..819
                     /locus_tag="ACHKCL_003919"
     CDS             <1..819
                     /locus_tag="ACHKCL_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO5764794.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(869..1111)
                     /locus_tag="ACHKCL_003920"
     CDS             complement(869..1111)
                     /locus_tag="ACHKCL_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5764795.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            1243..2526
                     /gene="ydfJ"
                     /locus_tag="ACHKCL_003921"
                     /pseudo
     CDS             1243..2526
                     /gene="ydfJ"
                     /locus_tag="ACHKCL_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2615..4075
                     /gene="ydfI"
                     /locus_tag="ACHKCL_003922"
     CDS             2615..4075
                     /gene="ydfI"
                     /locus_tag="ACHKCL_003922"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5764796.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(4111..4314)
                     /gene="ydfZ"
                     /locus_tag="ACHKCL_003923"
     CDS             complement(4111..4314)
                     /gene="ydfZ"
                     /locus_tag="ACHKCL_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5764797.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4491..5177)
                     /gene="rspR"
                     /locus_tag="ACHKCL_003924"
                     /gene_synonym="ydfH"
     CDS             complement(4491..5177)
                     /gene="rspR"
                     /locus_tag="ACHKCL_003924"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5764798.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(5266..6012)
                     /gene="ydfG"
                     /locus_tag="ACHKCL_003925"
     CDS             complement(5266..6012)
                     /gene="ydfG"
                     /locus_tag="ACHKCL_003925"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5764799.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            6149..8194
                     /gene="dcp"
                     /locus_tag="ACHKCL_003926"
     CDS             6149..8194
                     /gene="dcp"
                     /locus_tag="ACHKCL_003926"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5764800.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(8239..8757)
                     /gene="ydeJ"
                     /locus_tag="ACHKCL_003927"
     CDS             complement(8239..8757)
                     /gene="ydeJ"
                     /locus_tag="ACHKCL_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO5764801.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2199 a         2084 c         2242 g         2259 t
ORIGIN      
        1 gatgtctcac cggcgctggt atcgaaggtc accgatgccg ttatggagca ggtagtcgaa
       61 tggcaaaacc ggccactgga tgcggtctat cccattgttt atcttgactg tattgtcctg
      121 aaggttcggc aggacagtcg cgtaatcaat aaatccgtat tcctcgcact gggtatcaat
      181 atcgaaggcc agaaagagtt actgggtatg tggctggccg aaaacgaggg agcgaagttc
      241 tggctcaatg tgctgactga actaaaaaac cgcggcctga acgatattct catagcctgt
      301 gtcgacgggc tgaaaggctt cccggacgct atcaacacgg tgtatccgga agcccgcatc
      361 cagctgtgca tcgtgcatat ggtgcgcaac agcctgcggt tcgtttcctg gaaggactac
      421 aaagccgtca cccgcgacct gaaagccatc taccaggccc ccacagaaga agcaggccag
      481 caggcgctgg aagcgttcgc cagcgcctgg gacagtcgct acccgcagat aagccggagc
      541 tggcaggcaa actggaccaa cctggcgatg ttcttcgctt acccggcaga tatccgcaaa
      601 gtcatctaca caaccaacgc catcgagtcg ctgaacagcg taatccggca tgccatcaaa
      661 aaacgcaagg tgttcccgac ggatgactca gtgaaaaagg tcgtgtggct ggcaatccag
      721 gcggcttcac agaaatggac gatgccactg cgggactggc gtatggcaat gagccgcttt
      781 attatcgagt tcggtgaccg cctggacggt cacttctgag aaaaggcatt tacacagaat
      841 cgtgtacagg gtcgatttta ctcgttaatt agcgaacaaa ccactcgtca gcagactccc
      901 aggtatcttt cagcgtctcc tgaacaaaag tttttgcaga atctttatcg gcggtgcgag
      961 taactgaaag gccatcgttg ctggtggatt tgatgatcac ttctacatcg tcataacgtt
     1021 tactgacacg tcgcagcatt tcttgttgca gggcaggaac agaacctttt ggcattttgt
     1081 tgattttggc tgcggggtat ctgtggggca tatatgggac ataaaaaggc ctcaaataaa
     1141 cagcaaaatc gagattaggg atttttagaa aaaatacaac catctgaaaa aacttagaaa
     1201 acacccaaaa acccacagaa tagtaaaaaa catctatgaa ttatggattt ccagttatat
     1261 tcgctcggcg cagcgttagt gtttcatgaa atattttttc ctgaatcatc aacggcaatg
     1321 gcgttaattc tggcaatggg aacctacggt gcaggttatg tggcgcgtat tgtcggagca
     1381 tttattttcg gcaaaatggg cgacagaata gggcgtaaaa aagtgctctt tattaccatc
     1441 accatgatgg ggatctgtac caccttaatt ggagtgttac cgacctatgc acagattggt
     1501 gtttttgccc ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg
     1561 gaaatttccg gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt
     1621 atctcctcat ttgtggctat gggaactaac tgcggaacct tgagcgcaac ggcaatctgg
     1681 gcctttatgt tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg
     1741 ttcctggcga gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa
     1801 agcccggtct ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct
     1861 ggtagcatat tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggca
     1921 ggtaactccg ggttaattca gactttcctt gcaggctatt tagtacagac gttattgttt
     1981 aacaaagcaa ttccaacaga tgcattgatg atcagttcga ttcccggctt tatgaccatt
     2041 ccgttccttg gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat
     2101 acctccgcga ttgtgctggc atggccaatg ctttctatca tcgtagataa aagctatgcc
     2161 ccgagcacca ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt
     2221 gctctggaaa acattaccat ggcagaaatg ttcggctgta aaaaccgctt tacccggatg
     2281 gctatttcta aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt
     2341 attttctgca ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca
     2401 gtgattggtt taatctctgc gctgaaaatg ccagaagtga aagaccgtga tttaagtgcg
     2461 ctggaagacg ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt
     2521 ctgtaaatcc tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca
     2581 tgtagcttgc catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa
     2641 agcgacactc cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt
     2701 tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt
     2761 cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt
     2821 acatcagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg
     2881 cgtcgttggc gtcgttaaaa aagccttgca cgtacagata gatggcttag aaaccgtgtt
     2941 ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta
     3001 tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg ctgccgacgt
     3061 gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg
     3121 taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg
     3181 tcatgttatg cgtgacgttg tcacttccta cgcacaagcc gttgatgtaa aactagcaca
     3241 atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt
     3301 gacagaggat acgctggcga aaatcgaaca gcttaccggt gtgcgcgatc ctgctggcgt
     3361 tgcctgtgaa cctttccgcc agtgggtaat cgaagataac tttgttgccg gacgtccgga
     3421 atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt
     3481 gcgcatgctc aacggcagtc attcattcct ggcgtatctg ggttatcttg ccggatatca
     3541 gcacattaat gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg ccttgatgtt
     3601 gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg
     3661 attaattgca cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga
     3721 tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga
     3781 cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt
     3841 tgatgaacag ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc
     3901 tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcgat
     3961 ttttggtggt gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt
     4021 gtctttatta gcgcatggtg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa
     4081 gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa tcaggtccag
     4141 tggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc
     4201 agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac
     4261 cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt
     4321 tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt
     4381 cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc
     4441 ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa ttactcttcg
     4501 ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa
     4561 tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaatata
     4621 tcaagatgct ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc
     4681 acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca
     4741 atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg
     4801 cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg
     4861 gtaatcatac tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg
     4921 ccgttgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt
     4981 tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca
     5041 ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg
     5101 acaatgtcgc gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt
     5161 tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact
     5221 tttttgtgac aagcaataac ccggcagtta caccgggtat aaaaattact gacggtggac
     5281 attcagcccg gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg
     5341 agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg ttaatgcaac
     5401 ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa
     5461 ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag
     5521 gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt
     5581 accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat gaccatgatt
     5641 acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt tgttgttggt
     5701 atcaatcatc gtttcccagt cttcaatgct ggctttatgc gcaggctcca tgcccaacgc
     5761 cagaccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa gcgatgccag
     5821 catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatgtaca gattatctcc
     5881 cagttcgtct tttaactcct gcaaccgttc ctggcgacgg ccagtggcga taactttatg
     5941 cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac
     6001 taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg
     6061 cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta
     6121 tggaacacct cgcgttcagg agaagaaaat gacaacaatt aatcctttcc ttgtgcaaag
     6181 cacactgccg tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc
     6241 attcgatgag ggaatacagc aaaagcgggc agaaattgct gccatcgcgc ttaacccgca
     6301 aacacctgat ttcaacaata ctattctggc actggaacaa agcggagaat tacttacccg
     6361 cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct
     6421 tgacgagcag ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga
     6481 attattcgcg cgggtagatg ctgtctggca acgccgtgaa tctctggggc ttgatagtga
     6541 atccatccgc ctggtggagg tgattcatca acgttttgtc ctagccggag ccaaacttgc
     6601 acaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgacac tgaccagtca
     6661 gtttaaccag cggttactgg cagcaaataa atccggcggt ctggttgtga acgatatcgc
     6721 gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa
     6781 aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc
     6841 cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga
     6901 aaaaaatgat gccaatgata cccgcgctat cattcaacgt ctggtggaga tccgtgcaca
     6961 acaggctaca ttactcggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc
     7021 aaaaacacct gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg
     7081 tgcgagcgat gaattagcct ccatacaggc ggttattgat aagcagcagg gcgggtttag
     7141 cgcgcagccg tgggactggg cattttatgc cgaacaggtc cggcgagaga aatttgatct
     7201 tgatgaggcg cagctcaagc catattttga attaaacacg gtgttgaatg aaggtgtatt
     7261 ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta
     7321 ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc tggcgttatt
     7381 ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt
     7441 tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa
     7501 acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtcataacct tattccatga
     7561 atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tctccggcac
     7621 caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca
     7681 tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga
     7741 actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact
     7801 gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca
     7861 ggatgtcgat gattttgaat tgcgggcgct ggtggcagaa aatatggatc ttcctgctat
     7921 accgccacgc tatcgcagca gttattttgc ccatattttt ggtggcggat atgccgcagg
     7981 ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga
     8041 gcagggcgga ttaacgcgtg aaaatgggct gcgttttcgc gaggcgatcc tttccagagg
     8101 taacagcgaa gatctggaac gcctgtatcg acaatggcgc ggtaaggctc ctcagattat
     8161 gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg tcctggagat
     8221 atcgccaggc cagcctggtt atagcagtaa ctgcagcagc tgggcgaggg caaacctcac
     8281 cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg
     8341 gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc
     8401 cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc
     8461 ggttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagattgctg
     8521 gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta
     8581 aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga
     8641 ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag
     8701 gttttcaata gtgtcggtat ctgcaagttg tacaatttta tttctattaa tgttcactgt
     8761 tttttgtcca cgcttttata tgta
//
LOCUS       DAZFKY010000183         8740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFKY010000183 DAZFKY010000000
VERSION     DAZFKY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(293..505)
                     /gene="rpmE"
                     /locus_tag="ACHKCL_003928"
     CDS             complement(293..505)
                     /gene="rpmE"
                     /locus_tag="ACHKCL_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5764802.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            708..2906
                     /gene="priA"
                     /locus_tag="ACHKCL_003929"
     CDS             708..2906
                     /gene="priA"
                     /locus_tag="ACHKCL_003929"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5764803.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3062..4087
                     /gene="cytR"
                     /locus_tag="ACHKCL_003930"
     CDS             3062..4087
                     /gene="cytR"
                     /locus_tag="ACHKCL_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5764804.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4179..5138
                     /gene="ftsN"
                     /locus_tag="ACHKCL_003931"
     CDS             4179..5138
                     /gene="ftsN"
                     /locus_tag="ACHKCL_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5764805.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5231..5761
                     /gene="hslV"
                     /locus_tag="ACHKCL_003932"
     CDS             5231..5761
                     /gene="hslV"
                     /locus_tag="ACHKCL_003932"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5764806.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5771..7102
                     /gene="hslU"
                     /locus_tag="ACHKCL_003933"
     CDS             5771..7102
                     /gene="hslU"
                     /locus_tag="ACHKCL_003933"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5764807.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7169..8095
                     /gene="menA"
                     /locus_tag="ACHKCL_003934"
     CDS             7169..8095
                     /gene="menA"
                     /locus_tag="ACHKCL_003934"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5764808.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8188..8673
                     /gene="rraA"
                     /locus_tag="ACHKCL_003935"
     CDS             8188..8673
                     /gene="rraA"
                     /locus_tag="ACHKCL_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5764809.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2122 a         2280 c         2385 g         1953 t
ORIGIN      
        1 acgtcactcc gccggggcac accgaacagg ccacgccggt gggggcacca atgcgcaccc
       61 cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcgccctga cgcgccgccg
      121 gttttccgct catcgtaaaa tccttttgaa ataaataaga accagaagcc tagcgccagt
      181 caacaacagg aaatgataat gagttaacac cccggaattt ttcagaattt ataaaaggca
      241 aattacaggc acaaaaaaag cgccgtgcgg cgcttttttc ggaaatccgg tcttatttgc
      301 tgcccgggat gttgaaacgc ttgttgaagc ggtcaacacg gccaccggta gcaacatcac
      361 gctgtttgcc agtgaagaac gggtggcact tgctgcacac gtcgaggttc aggtcatgac
      421 caacggtgga gcggattttc attacgttac cgcaagagca gctagcagta atttcttcgt
      481 atttcgggtg aatatctttt ttcatgggaa aacctcggtt taaggccgcg tcgctcttcc
      541 agccctaacg ccagacacca cgcgatgtta aaagtatagc ttcaatacga tcatttcgta
      601 cgaaggcgcg aaatcataca gaaattaacc agcatatgca aactgatccg cactcttcta
      661 cggcaatgtg tatactaacc caccgaattt caagtcagga tgatgctatg cccgttgccc
      721 acgttgcctt gcccgttccg cttcctcgta cctttgacta tctcctgcca gaaggcatgg
      781 cggttaaagc cgggtgtcgc gtgcgcgtgc cgtttggcaa acagcaggag cgcatcgggg
      841 ttgtggtgtc agttagcgac gtcagtgagc tgccgcttaa cgagctaaaa gcggtagtcg
      901 aagtgctgga tagtgagccg gtgtttactc actccgtctg gcgattgctg ctctgggcgg
      961 cggattacta tcatcatccg attggcgatg tgctgtttca tgccttgccg attttactgc
     1021 gccaggggcg gcctgcggcg aacgcgccga tgtggtactg gtttgccact gaacaaggcc
     1081 atgcggtgga tctgaacagc ctgaaacgtt cgccaaagca acaacaggca ctggcggcat
     1141 tgcggcaagg caaaatctgg cgcgaccagg ttgccacgct tgaatttaat gatgccgcgt
     1201 tacaggcgct gcgcaaaaaa ggtctgtgtg atttagcaag tgaaacacca gagtttagcg
     1261 actggcgaac gaactatgcc gtttctggtg aacggttgcg attgaatacc gaacaggcca
     1321 ccgccgttgg cgcaattcat agcgcggcgg ataccttctc tgcttggctg ctggcgggcg
     1381 ttacaggttc cggtaaaaca gaggtttatt tgagcgtact ggaaaacgtg ctcgctcagg
     1441 gcaaacaggc gctggtgatg gtgccggaaa tcggcctgac accgcaaact atcgcccgtt
     1501 ttcgtgaacg ttttaatgcc cccgtagaag ttctgcattc cggcctgaac gacagcgagc
     1561 gtctttcggc gtggttgaaa gcgaaaaatg gtgaggcggc gattgtcatc ggcacccgct
     1621 ccgcgctgtt tacgccgttt aaaaatctcg gtgtgattgt cattgatgaa gagcacgaca
     1681 gttcctacaa gcagcaggaa ggctggcgct atcatgcccg cgacctggcg gtttatcgtg
     1741 cgcacagcga gcaaatcccg attattcttg gctccgcaac acccgcactg gaaacgttat
     1801 gcaacgtcca gcagaaaaaa taccgcctgc tgcgcctgac gcgtcgggcg gggaatgcgc
     1861 gtccggcaat tcaacatgtg ctggatttaa aaggtcagaa ggtgcaggcg gggctggctc
     1921 cggcgttaat cacccgtatg cgccagcatt tacaggccga caaccaggtt atcctctttc
     1981 ttaaccgccg tggctttgcg cctgccttgc tgtgccacga ctgcggctgg attgccgaat
     2041 gtccacgttg cgatcactac tacacgctgc atcaggcgca acagcatttg cgctgtcacc
     2101 actgtgacag ccaacgcccg gtgccgcgcc agtgcccttc ctgcggttcc acgcacctgg
     2161 tacccgtggg gctgggcacc gaacagcttg aacagacgct cgcgccgtta ttccctggtg
     2221 tgcccatttc tcgtatcgac cgcgatacca ccagccgcaa aggagcgctg gaacaacaac
     2281 tggcggaagt gcatcgcggc ggcgcgagga tattgattgg cacgcaaatg ctggcgaaag
     2341 gtcaccactt tccggatgtg acgctggtag cgttactgga tgtggacggc gcgctgtttt
     2401 ctgccgattt tcgctcggca gagcgttttg cccagctata cacccaggta gcaggccgtg
     2461 ccgggcgtgc gggtaaacag ggcgaagtgg tgctgcaaac gcatcatccg gaacacccgc
     2521 tattgcaaac gttgctctat aaaggctacg acgcttttgc cgaacaggca ctggctgagc
     2581 ggcgaatgat gcaactgccg ccgtggacca gccatgtgat tgtgcgtgcg gaagatcata
     2641 acaatcagca cgcgccattg ttcctgcaac aactgcgtaa tctgatcctc tccagcccgc
     2701 tggcagacga caaattgtgg gttctcggtc cggttccggc actggcacct aaacgtggcg
     2761 gtcgctggcg ctggcagata ttgttgcagc acccttcccg cgtgcgcttg caacacatca
     2821 ttaacggtac gctggcactc atcaatacaa taccggattc ccgtaaggtg aaatgggtgc
     2881 tggatgttga tccgattgag ggttaaaccg ctcacgatgc gaggcggatc gaaaaattca
     2941 atattcatca cacttttcat gaaaattctg taaccgtttt cacgcgctat ctgctaaaaa
     3001 tgttgccgat gtgaagtaaa catggatgta gtacgcctga cgtgccaggc gaggagtgag
     3061 tgtgaaagcg aagaagcagg aaactgccgc gaccatgaaa gacgttgccc tcaaggcaaa
     3121 agtctctaca gcgaccgtct cccgagcatt aatgaatccc gataaagtct cccaagccac
     3181 ccgtaatcgg gttgaaaaag cggcccgtga agtgggttat ttaccgcagc ctatggggcg
     3241 caacgtcaag cgtaatgaat cccgcaccat tctggtgatt gtcccggata tctgcgatcc
     3301 cttctttagc gaaattattc gcggtatcga agttacggcg gcaaatcacg gatatctggt
     3361 gctgattggc gactgtgcgc atcaaaatca gcaggaaaaa acctttatcg atttgatcat
     3421 caccaagcaa attgatggca tgttgctgct gggttcaagg ctgccgtttg atgccagcat
     3481 tgaggaacag cgtaatctgc cgccgatggt gatggcgaac gaatttgcac cggaactgga
     3541 gctgcctacc gttcatatcg acaatctgac cgccgcattt gatgcagtaa attatttata
     3601 tgagcaaggg cataaacgga ttggctgtat agccggtccc gaagagatgc cgctgtgtca
     3661 ctaccgcctg caaggttacg ttcaggcgct gcgtcgctgc ggcattatgg ttgatccgca
     3721 atacatcgcc cgtggcgact tcaccttcga agccggaagc aaagcgatgc agcagctgct
     3781 tgatcttcca caaccgccta ctgctgtctt ctgccatagc gatgtgatgg cgctcggcgc
     3841 actttctcag gcaaaacgcc aggggctgaa agtcccggaa gacctttcca taatcggttt
     3901 tgataacatc gacctgacgc aattttgtga tccgccgctg acaaccatcg cgcagccgcg
     3961 ttacgaaatc ggtcgggaag ctatgctgtt attgcttgat caaatgcagg ggcaacacgt
     4021 tggcagtggc tctcgtttaa tggactgcga acttatcatc cggggatcaa cacgcgcgtt
     4081 accttaaagt aaacctttaa gactgccgtg actggtcaaa ggcccgccgc ttaagtaaca
     4141 tggcgggctg acgaacgaat aaatacagcg aaacgatagt ggcacaacga gattatgtac
     4201 gccgcagcca accggcacct tcgcggcgaa aaaagagcac ctcacggaaa aagcaacgaa
     4261 atctgcctgc ggtttctccc gctatggtcg ctattgctgc cgccgttctt gtgaccttta
     4321 tcggtggtct gtacttcatt acgcatcaca agaaagaaga gtccgagacg ctgcaaagcc
     4381 agaaagtgac cggaaacgga ctaccaccaa aaccagaaga acgctggcgc tacatcaaag
     4441 agctggaaag tcgccagccg ggtgtgcgtg cgcccacaga accttctgcc ggtggtgaag
     4501 tgaaaacgcc ggagcaactg acaccagaac aacgtcagct tcttgaacaa atgcaggctg
     4561 atatgcgcca gcagccaacg cagctggttg aagttccgtg gaatgaacag acgccggaac
     4621 agcgtcagca aacgctacag cgccaacgtc aggcgcagca attagcggaa cagcaacgcc
     4681 tggctcagca gtcgcgtacg actgaacaaa gctggcagca gcagacgcgt acgtcgcaag
     4741 ccgcgccggt acaggcgcaa ccgcgccagt ctaaaccggc ttctactcag cagccgtacc
     4801 aggatctgct gcaaacccct gcgcacacga ccgcgcagtc gaagccgcag caagctgcgc
     4861 cggttactcg tgccgctgac gcgccaaaac cgacggcgga gaaaaaagac gaacgccgct
     4921 ggatggtgca gtgcggttcg ttcagaggcg cggaacaggc agagacggta cgtgctcagc
     4981 tggcgttcga aggctttgac tcaaaaatta ccaccaacaa tggctggaat cgtgtggtca
     5041 ttggcccggt gaaaggcaaa gagaacgcag acagcaccct caatcggttg aagatggcgg
     5101 gtcatacaaa ctgcattcgg ctcgccgccg ggggttgaaa ccctcaaaat cccccccatc
     5161 tataattgca ttatgccccg tacttttgta cggggtttgt actctgtatt cgtaaccaag
     5221 gggtcagctc gtgacaacta tagtaagcgt acgccgtaac ggccatgtgg tcatcgctgg
     5281 tgatggtcag gccacgttgg gcaataccgt aatgaaaggc aacgtgaaaa aggtccgccg
     5341 tctgtacaac gacaaagtca tcgcgggctt tgcgggcggt actgcggatg cttttacgct
     5401 gttcgaactg tttgaacgta aactggaaat gcatcagggc catctggtca aagccgccgt
     5461 tgagctggca aaagactggc gtaccgatcg catgttgcgc aaacttgaag cactgctggc
     5521 agtcgcggat gaaactgcat cgcttatcat caccggtaac ggtgacgtgg tgcagccaga
     5581 aaacgatctt attgctatcg gctccggcgg cccttacgcc caggctgcgg cgcgtgcact
     5641 gttagaaaat actgaactta gtgctcgtga aattgctgaa aaggcgttgg atattgcagg
     5701 cgacatttgc atctatacca accatttcca caccatcgaa gaattaagct acaaagcgta
     5761 aggatctccc atgtctgaaa tgaccccacg cgaaatcgtc agcgaactgg ataagcacat
     5821 catcggccag gacaacgcca agcgttccgt ggcgattgct ctgcgtaacc gctggcgtcg
     5881 catgcagctc aacgaagagc tgcgccatga agtgaccccg aaaaatatcc tgatgatcgg
     5941 cccgaccggt gtcggtaaaa ctgaaatcgc ccgtcgtctg gctaagctgg cgaatgcgcc
     6001 gttcatcaaa gttgaagcga ccaaattcac cgaagtgggc tacgtcggta aggaagtgga
     6061 ttctattatt cgcgatctga ccgatgccgc cgtgaaaatg gtacgcgtcc aggctatcga
     6121 gaaaaaccgt tatcgcgctg aagaactggc agaagaacgt attctcgacg tgctgatccc
     6181 acctgctaaa aacaactggg gacagaccga acagcagcag gaaccgtccg ctgctcgtca
     6241 ggcattccgc aaaaaactgc gtgaaggcca gcttgatgac aaagaaatcg agatcgatct
     6301 tgccgcagca ccgatgggcg ttgaaattat ggctcctcca ggcatggaag agatgaccag
     6361 ccagctgcag tccatgttcc agaacctggg cggtcagaag caaaaagcgc gtaagctgaa
     6421 aatcaaagac gccatgaagc tgctgattga agaagaagcg gcgaaactgg tgaacccgga
     6481 agagctgaag caagacgcta tcgacgctgt tgagcagcac gggatcgtgt ttatcgacga
     6541 aatcgacaaa atctgtaagc gcggcgagtc ttccggtccg gatgtttctc gtgaaggcgt
     6601 tcagcgagac ctgctgccgc tggtagaagg ttgcaccgtt tccaccaaac acgggatggt
     6661 caaaactgac cacattctgt ttatcgcttc tggcgcgttc cagattgcga aaccgtctga
     6721 cctgatcccg gaactgcaag gtcgactgcc aatccgcgtt gaactgcagg cactgaccac
     6781 cagcgacttc gagcgtattc tgaccgagcc gaatgcctct atcaccgtgc agtacaaagc
     6841 actgatggcg actgaaggcg taaatatcga gtttaccgac tccggtatta aacgcatcgc
     6901 ggaagcggca tggcaggtga acgaatctac cgaaaacatc ggtgctcgtc gtttacacac
     6961 tgttctggag cgtttaatgg aagagatttc ctacgacgcc agcgatttaa gcggtcaaaa
     7021 tatcactatt gacgcagatt atgtgagcaa acatctggat gcgttggtgg cagatgaaga
     7081 tctgagccgt tttatcctat aatcgcgttc aatcattttc atcattgttt gatggggctg
     7141 aaaggcccca tttttattgg cgcgtattat gactgaacaa caaattagcc gaactcaggc
     7201 gtggctggaa agtttacgac ctaaaaccct ccccctcgcc tttgctgcaa ttatcgtcgg
     7261 gacggcgctg gcatggtggc aaggtcattt tgatccgctg gtcgccctgc tggcactgat
     7321 taccgccggg ctattacaga tcctttctaa cctcgccaat gattacggcg atgcggtaaa
     7381 aggcagcgat aaacctgacc gtatcggacc gctacgcggc atgcaaaaag gggtcattac
     7441 ccagcaagag atgaaacggg cgctcattat taccgtcgtg ctcatctgtc tctccgggct
     7501 ggcactggtt gcagtggcgt gccatacgct ggccgatttt gtcggtttcc tgattcttgg
     7561 cgggttgtcg atcattgccg ctatcaccta caccgtgggc aatcgtcctt atggttatat
     7621 cggtctgggt gatatttccg tactggtttt ctttggctgg ttaagcgtca tggggagctg
     7681 gtatttacag gctcatacat tgattccggc actgatcctt ccggcgaccg catgcggcct
     7741 gctggcaacg gcagtactga acattaataa cctgcgtgat atcaatagcg accgcgaaaa
     7801 cggcaaaaac acgctggtgg tgcgcttagg tgaagtgaac gcgcgtcgtt atcatgcctg
     7861 cctgctgatg ggctctctgg tatgtctggc gctgtttaat ctcttttcgc tgcatagcct
     7921 gtggggctgg ctgttcctgc tggcggcacc gttgctggtg aagcaagccc gttatgtgat
     7981 gcgggaaatg gacccggtgg cgatgcgacc gatgctggaa cgcactgtca agggagcgtt
     8041 actgactaac ctgctgtttg ttgtagggat attcctaagc cagtgggcag cataactgac
     8101 aaatatcaat taacaattga tgattttgcc aacagaccac atagcgcgat atactgaaaa
     8161 ttctcgcagc aactgaatgt taagcctatg aaatacgata cttccgagct ttgtgacatc
     8221 tatcaagaag atgttaacgt cgtggaaccg ctgttctcca actttggcgg acgggcgtcg
     8281 tttggcggac aaataatcac ggtaaaatgt ttcgaggaca acgggttact gtacgatctg
     8341 ctcgaacaga atggccgtgg tcgtgttctt gtcgtcgatg gcggtggttc tgttcgtcgc
     8401 gcactggtcg atgctgaact ggcgcgtctg gcagtacaaa atgaatggga aggtctggtc
     8461 atttacggcg cggtgcgtca ggtagatgac ctggaagagt tggatatcgg catccaggcg
     8521 atggcggcaa ttccggttgg tgccgctggc gaaggcattg gcgaaagcga tgtccgcgtc
     8581 aattttggcg gtgttacctt cttctccggc gaccatcttt atgccgacaa taccgggatt
     8641 attctttcag aagatccgct ggatattgaa taagtatctg ccatgcgtta aaaggggaat
     8701 taaattcccc ttttttatac atggacgtat tcatgcagaa
//
LOCUS       DAZFKY010000184         8692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFKY010000184 DAZFKY010000000
VERSION     DAZFKY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(334..597)
                     /gene="rpsT"
                     /locus_tag="ACHKCL_003936"
     CDS             complement(334..597)
                     /gene="rpsT"
                     /locus_tag="ACHKCL_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5764810.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            700..924
                     /locus_tag="ACHKCL_003937"
     CDS             700..924
                     /locus_tag="ACHKCL_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5764811.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            932..1873
                     /gene="ribF"
                     /locus_tag="ACHKCL_003938"
     CDS             932..1873
                     /gene="ribF"
                     /locus_tag="ACHKCL_003938"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5764812.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1916..4732
                     /gene="ileS"
                     /locus_tag="ACHKCL_003939"
     CDS             1916..4732
                     /gene="ileS"
                     /locus_tag="ACHKCL_003939"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5764813.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4732..5226
                     /gene="lspA"
                     /locus_tag="ACHKCL_003940"
     CDS             4732..5226
                     /gene="lspA"
                     /locus_tag="ACHKCL_003940"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5764814.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5314..5763
                     /gene="fkpB"
                     /locus_tag="ACHKCL_003941"
     CDS             5314..5763
                     /gene="fkpB"
                     /locus_tag="ACHKCL_003941"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5764815.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5765..6715
                     /gene="ispH"
                     /locus_tag="ACHKCL_003942"
                     /gene_synonym="lytB"
     CDS             5765..6715
                     /gene="ispH"
                     /locus_tag="ACHKCL_003942"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5764816.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6781..7695
                     /gene="rihC"
                     /locus_tag="ACHKCL_003943"
     CDS             6781..7695
                     /gene="rihC"
                     /locus_tag="ACHKCL_003943"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5764817.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7862..8683
                     /gene="dapB"
                     /locus_tag="ACHKCL_003944"
     CDS             7862..8683
                     /gene="dapB"
                     /locus_tag="ACHKCL_003944"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5764818.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1993 a         2170 c         2459 g         2070 t
ORIGIN      
        1 tacaaggtct aatgaggaga tatttatgaa tggttaaagt atataggaat ttaattcaaa
       61 aatagagtgg tcttaagttt aatacctcaa tgtgtatcac aatatccaca ttctttgtag
      121 gggagtctgg agattgagta gatattcttg ttcagaatgt atcagccgat ggttctacga
      181 ttcttaagcc acgaagagtt cagatagtgc aacggcatgt ctcttttgac tatctggcaa
      241 ccggcagtgt gttctctcac gcatcacaaa agcagcaggc ataaaaaaac ccgcttgcgc
      301 gggctttttc acaaagcttc agcaaattgg caattaagcc agtttgttga tctgtgcagt
      361 caggttagcc ttatgacgtg cagctttgtt tttgtggatc agacctttag cagcctgacg
      421 gtccacgatc ggttgcattt cgttaaatgc tttctgtgca gcagctttgt cgccagcttc
      481 gatagctgcg tatactttct tgatgaaagt acgcatcata gagcgacggc ttgcgttgtg
      541 cttacgagcc ttttcagact gaatggcgcg cttcttagct gatttgatat tagccaaggt
      601 ccaactccca aatgtgttct atatggacaa ttcaaaggcc gaggaatatg cccttttagc
      661 cttcttttgt caatggattt gtgcaaataa gcgccgttaa tgtgccggca ctcgttacgt
      721 agtgatggcg caggattcta ccagcttgcg gggtgtgaat acagcttttc cgcgataaaa
      781 attgcagcag gcagtcagtt tcttcccgtg atttgcgcca tgaccatgac aatgaaaagc
      841 cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata
      901 ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct
      961 cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg
     1021 cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt
     1081 gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc
     1141 acggctgacc cgactgcggg aaaaactacg ttaccttgca gagtgtggcg ttgattacgt
     1201 gctgtgcgta cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga
     1261 tcttctggtg aagcatttgc gggtaaaatt cctcgccgtg ggggatgatt tccgctttgg
     1321 cgctggtcgg gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga
     1381 tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca ccgccgtgcg
     1441 tcaggccctt gcggatgaca atttggctct ggcagaaagt ttactggggc acccgtttgc
     1501 catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc
     1561 gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct
     1621 gggcctcggt gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc
     1681 cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg
     1741 ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cttcgctgga
     1801 cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac
     1861 aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag
     1921 tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc
     1981 caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcattcg
     2041 tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg
     2101 cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa
     2161 agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat
     2221 cgagctgaaa gtagagcaag aatacggtaa gccggatgag aaattcaccg ccgccgagtt
     2281 ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat
     2341 ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact tcaaaactga
     2401 agccaatatc atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc
     2461 gaagccggtg cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta
     2521 ttacgacaaa acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct
     2581 gaaagcgaaa tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac
     2641 tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc
     2701 gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat
     2761 gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cggagcttga
     2821 gctgctgcgc tttgcccatc cgtttatggg cttcgacgtc ccggcaatcc tcggcgatca
     2881 cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga
     2941 ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac
     3001 ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat
     3061 cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag
     3121 ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcaacgc cgcagtggtt
     3181 cgtcagtatg gatcagaaag gtctgcgcgc gcagtctctg aaagagatca aaggtgtgca
     3241 gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gtcgctaacc gtcctgactg
     3301 gtgtatctcc cgtcagcgta cctggggcgt accgatgtct ctgttcgtgc acaaagacac
     3361 ggaagagctg catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt
     3421 tgatggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg atgaagctga
     3481 tcagtatgtg aaagtgccgg atacgctgga tgtatggttt gactccggct ctactcactc
     3541 ttctgttgtt gacgtgcgcc cggaatttgc cggtcacgct gcggacatgt atctggaagg
     3601 ttctgaccaa caccgtggct ggttcatgtc ttctctgatg atctccaccg cgatgaaggg
     3661 caaagcgccg tatcgtcagg tgctgaccca cggctttacc gtagatggtc agggccgcaa
     3721 gatgtctaaa tccatcggca acaccgtgtc gccgcaggat gtgatgaaca aactgggcgc
     3781 ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga
     3841 cgagatcctg aaacgtgctg ccgacagcta tcgtcgtatc cgtaacaccg cgcgcttcct
     3901 gctggcaaac ctgaacggtt ttgatccggc aaaagatatg gtgaaaccgg aagagatggt
     3961 agtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc
     4021 atacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga
     4081 gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggacag
     4141 cgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gcagaagcgc tggttcgctg
     4201 gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg
     4261 tgaaaaatac gtcttcaccg gcgagtggta cgaaggtctg tttggtctgg cagacagtga
     4321 agcaatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt
     4381 cattgagcag gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt
     4441 gtatacagaa ccggaactgg cggcgaaact gaccgcattg ggcgatgaat tacgatttgt
     4501 cctgttgacc tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca
     4561 gagcgaagtg ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc
     4621 acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg
     4681 ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa
     4741 tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat
     4801 ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc
     4861 ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat
     4921 agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgttaccctg
     4981 gttgtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ctacgcgctg
     5041 attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat
     5101 atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaacctcgc cgatactgcc
     5161 atctgtgttg gtgcggcact gattgtgctg gaaggttttt tgccttctaa agcgaaaaaa
     5221 caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag gccgcatccg
     5281 gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca gagcaatagc
     5341 gccgtcctgg tgcacttcac gctaaaactc gacgatggca ccaccgccga gtctacccgc
     5401 aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttccga agggctggag
     5461 caacacctgc tggggctgaa agtgggcgat aaaaccactt tctcgctgga gccagatgcg
     5521 gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga gtttatggat
     5581 gcaggcgagc cagaaattgg cgcaataatg ctttttaccg caatggatgg cagtgagatg
     5641 cctggcgtga tccgcgaaat taacggcgac tccattaccg tcgatttcaa ccatccgctg
     5701 gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc actggaggcg
     5761 taacatgcag atcctgttgg ccaacccacg tggtttttgt gccggggtag accgcgctat
     5821 cagcattgtt gaaaacgcgc ttgccattta cggcgcaccg atatatgtcc gtcacgaagt
     5881 ggtgcataac cgctacgtgg tcgatagcct gcgcgagcgt ggagctatct ttattgagca
     5941 gatcagcgaa gtgccggacg gcgcgatcct gatcttctcc gcacatggtg tttctcaggc
     6001 ggtacgtaac gaagcgaaaa gccgtgattt gacggtattc gacgccacct gtccgctggt
     6061 gaccaaagtg catatggaag tcgcccgcgc cagccgtcgt ggcgaagagt ctattctcat
     6121 cggtcacgcc gggcacccgg aagtggaagg gacgatgggg cagtacagca accctgaagg
     6181 gggaatgtat ctggtcgaat cgcctgacga tgtgtggaaa ctgacggtca aaaacgaaga
     6241 gaagctctcc tttatgaccc aaaccacgct gtcggtagat gacacgtctg atgtgatcga
     6301 cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca tctgctacgc
     6361 cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag ttgtgttggt
     6421 ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc agcgtatggg
     6481 caaacgcgcg tttttgattg acgatgcgaa agatatccag gaagagtggg tgaaagaggt
     6541 taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc agaatgtggt
     6601 ggcacgtttg cagcagctgg gtggtggtga agccattccg ctggaaggcc gtgaagaaaa
     6661 tattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg attaagtcat
     6721 tagcagccta agttatgcga aaatgccggt cttgttatcg gcatttttta tggagaaaac
     6781 atgcgcttac ctatcttcct cgatactgac cccggcattg acgatgccgt cgccattgcc
     6841 gccgcgattt ttgcccccga actcgacctg caactgatga ccaccgtcgc gggtaatgtc
     6901 tcggttgaga aaactacccg taacgccttg caactgctgc atttctggaa tgcggagatt
     6961 ccgctcgccc agggagccgc tgtgccgctg gtacgcgcac cgcgtgatgc agcatctgtg
     7021 cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa gccactcggg
     7081 ataccggcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc cgttaccctg
     7141 gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc ggaatgtaaa
     7201 ccgtatattc gccgtctggt gatcatgggt ggttctgccg gacgcggcaa ctgtacgcca
     7261 aacgccgagt ttaatattgc tgccgatcca gaagctgctg cctgtgtctt ccgcagtggt
     7321 attgaaatcg tcatgtgcgg tttggatgtc accaatcagg caatattaac tcctgactat
     7381 ctctctacac tgccgcagtt aaaccgtacc gggaaaatgc ttcacgccct gtttagccac
     7441 taccgtagcg gcagtatgca aagcggcttg cgaatgcacg atctctgcgc catcgcctgg
     7501 ctggtgcgcc cggacctgtt cactctcaaa ccctgttttg tggcagtgga aactcaaggc
     7561 gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa accagccaat
     7621 gtacaggtgg cattggatct gaatgtaaaa ggcttccaac agtgggtggc tgaggtgctg
     7681 gctctggtgc cgtaacctgt cacatgttat cggcatgcag tcattcatcg actcatgcct
     7741 ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt ttggctggcg
     7801 gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa agagaatagc
     7861 tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta tgggccgcca
     7921 gttgattcag gcggcactgg cattagaagg cgtgcagctg ggcgcggcgc tggagcgcga
     7981 aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga aaacaggcgt
     8041 taccgtgcaa agcagtcttg atgcgataaa agatgatttt gatgtgttta tcgattttac
     8101 ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca aagggatggt
     8161 gatcggcact acggggtttg acgaagccgg aaaacaggca attcgtgacg ccgccgccga
     8221 tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc ttaagctgct
     8281 ggagaaagca gccaaagtga tgggtgacta caccgatatt gaaattattg aagcacatca
     8341 tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg cgatcgccca
     8401 cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc ataccggtga
     8461 acgtgtgcct ggtaccattg gttttgccac cgtgcgtgca ggtgacatcg ttggcgaaca
     8521 taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg cgtccagccg
     8581 catgacattt gctaacggtg cggtaagatc ggctttgtgg ttgagtggta aggaaaatgg
     8641 tctttttgat atgcgagatg tgcttgatct caataatttg taaccacaaa at
//
LOCUS       DAZFKY010000185         8588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFKY010000185 DAZFKY010000000
VERSION     DAZFKY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(228..1457)
                     /locus_tag="ACHKCL_003945"
     CDS             complement(228..1457)
                     /locus_tag="ACHKCL_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5764819.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            1706..2827
                     /gene="roxA"
                     /locus_tag="ACHKCL_003946"
                     /gene_synonym="ycfD"
     CDS             1706..2827
                     /gene="roxA"
                     /locus_tag="ACHKCL_003946"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5764820.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(2876..4102)
                     /gene="pepT"
                     /locus_tag="ACHKCL_003947"
     CDS             complement(2876..4102)
                     /gene="pepT"
                     /locus_tag="ACHKCL_003947"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5764821.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            4352..5488
                     /gene="potA"
                     /locus_tag="ACHKCL_003948"
     CDS             4352..5488
                     /gene="potA"
                     /locus_tag="ACHKCL_003948"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5764822.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5472..6335
                     /gene="potB"
                     /locus_tag="ACHKCL_003949"
     CDS             5472..6335
                     /gene="potB"
                     /locus_tag="ACHKCL_003949"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5764823.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            6699..8060
                     /gene="espK"
                     /locus_tag="ACHKCL_003950"
     CDS             6699..8060
                     /gene="espK"
                     /locus_tag="ACHKCL_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO5764824.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            8121..8396
                     /locus_tag="ACHKCL_003951"
     CDS             8121..8396
                     /locus_tag="ACHKCL_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO5764825.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(8476..>8588)
                     /locus_tag="ACHKCL_003952"
     CDS             complement(8476..>8588)
                     /locus_tag="ACHKCL_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO5764826.1"
                     /translation="YFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT         2329 a         2109 c         1910 g         2240 t
ORIGIN      
        1 tcatgttaat acaaaggcat aaaatattgt ttcatgcctt ttttctcgct ctttaaagag
       61 tgattcgcta gtgtataaaa aaccagtaac acattaaaaa tcagctactt ataaatccgt
      121 acgctttttc gcctcttgtt cgtggttgtt ccacattgtt gctcattgct gcacgttgta
      181 tctgttcgca tatccagtat gcgcatgctg aaaaaacacg aaaaaaatta ttttcttctg
      241 gctactggta gcgtggttac gttttcatgt gttcccgcca gtatccctaa ccgctccgtc
      301 cacatatcca gcgcattgcg tttagcatcc agataacggg aatgattata aactcgctgc
      361 attcctggca tctggtggcc tgtaagctgc tccacgacgt gaggatccac gcctaaatcg
      421 ttcagcatcg ttgtaaaggt gcgccggatg tcatgtagtg accagtgagg gtgatttagc
      481 ctcctatgcg ctaatctgcc gtactgcgac acgcttgttt cctgtttcac ttcccccagc
      541 aataaacccg tgtgcctgtt ctgctccacc agctgcgtga cgaacggcag tattgcttcc
      601 ggtatgggcc ggaatattgc caccttcgtt ttgctgtgtt ctttcggcac ggtccagagc
      661 atttcggtaa aatcccactc gctgatctcc gataacctca gttctaccgt ccggcatccg
      721 aacacaatca ggaggcggat taacgcgatg tagtaggggg agaatatttt tttgtccagt
      781 gcctgcaata attcgcccag ttctttggtg cttaagacac gctcgcttat atccggtttt
      841 ttcccaacgt ccgccacact catatcatca agaacgttgc tgattgcata gcgccgcctc
      901 cggcagaact taagcgcctg tttgcacgtc tgtagcaaga atccggcagt aacaggcgtt
      961 cgctttgcca cctggtcaaa acaggccagc cagtgccgta gctcgcattt atccagcggc
     1021 atagcaccaa tgtgctgtat tacgtgatta ttaaggcgct ttttcagggc ggcataatcc
     1081 acgcggtttt cctttgcgta cgactcaagc cagtaggtga gcgcatcgcc aaccgttacc
     1141 ggctttaacg cttcctgtac ggtgtaatta agctcatgac gtggattttt cccctctgcc
     1201 agccatgcgc gacactgggc ggctttttcc ctggctgatt tcaggctcag atcaggataa
     1261 tttcccagct taatgcgttc cggtggtgcc ccccttcccg ttccggccct gtaagtgaaa
     1321 taccaggtta aaaggccact ggttgaatgc ctgacgctca ggtttccacc gtcattaaga
     1381 aaggctgttt tttgggcggg tgtgccgttg attttcctca gctgtgtatc gctcagtttg
     1441 ttaagtgctc tgctcataat tttgattccg gtcatcacaa ttaactacac cattaactac
     1501 accgatcgtt gcacaaaggg ctacaacgtg aaacaagctg gaacaaggaa aatcacaaat
     1561 ctgtttataa tcaaaaacat aatgaacaat ctgaaacatt atgaaacggc aaaaaacact
     1621 aattgataaa atatgtccat acttcacgca ttacgttaag catccgttat aatcggttgc
     1681 agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact ggcccgattt
     1741 tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta ataattttat
     1801 tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag tcgacagtcg
     1861 actggtcagt caccaggatg gaaaatggca ggtcagccac ggtccgttcg aaagctacga
     1921 tcatctcggt gaaactaact ggtcattgtt agtgcaagca gtgaaccact ggcatgagcc
     1981 gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg atgatctgat
     2041 gatctctttt tctgtacctg gcggcggcgt cggcccgcat ctcgatcagt acgacgtgtt
     2101 tatcattcag ggtaccggac gtcgtcgctg gcgagtgggc gagaagctgc aaatgaaaca
     2161 gcactgccca catccggatc tgttacaggt cgatccgttc gaagccatca tcgatgaaga
     2221 gctggagcct ggcgatattc tttatattcc gccaggattc ccgcatgaag gctacgcgct
     2281 ggaaaatgcg atgaactatt ccgtgggttt tcgcgcgcca aatacgcggg aattaattag
     2341 cggatttgcc gattatgtgc tgcaacgcga actgggcggc aactactaca gcgatcctga
     2401 tgttccacct cgcgctcatc ctgcggacgt tctgccgcaa gagatggata aactgcgtga
     2461 gatgatgctc gaattgatca accagccgga acactttaag caatggtttg gcgagtttat
     2521 atcccagtca cgtcatgaac tggatatcgc gccgccggag ccgccttatc agccagatga
     2581 aatctacgat gcgctgaaac aaggtgatgt gctggtgcgc ctgggtggtc tgcgcgtatt
     2641 gcgcattggc gacgacgtgt ataccaatgg tgagaagatc gattccccgc accgtccggc
     2701 actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg atgcgctgga
     2761 agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt ggttcttcga
     2821 agggtaagtt tggatttaag ccggatgcgg catcacatgc cgcatccctt ttcgcttact
     2881 tccgttgcgc cgttaactcg gcaatacgga cgatcacctg caccgctttt tccatacctt
     2941 ccagagtcac aaactcatgc ttaccatgat agttgtaacc gccagtgaac aggttcgggc
     3001 acggtaatcc cataaacgac aactgcgcgc cgtcggtacc gccgcggatc ggtttcagtt
     3061 ccggttcaat atcgcagtca cgcatcgcct gctgggcgat atcgagaata tgcggatgct
     3121 caaccacttt ctcgcgcata ttgtagtaac tgtcttcaat caccaattca atgtagcaat
     3181 caggatgtaa ccctttgccc acttttttgg cgatctccat cattttacgt ttacgcgctt
     3241 caaactgttt acggtcgaaa tcacggatga tgtagtgcat atcggcccgt tcaacggtgc
     3301 ctttcatgct cgccaggtga tagaaacctt catagccttc tgtcatttcc gggctttcat
     3361 ccgccggaac ttccgcatga atacgtgccg ccagcgacag cgcatttacc atcactcctt
     3421 tcgccgtgcc cggatgaaca ttgttaccga caattttgat attgaccgat gcggcgttga
     3481 agttttcaaa ctccagttcg cctacgccac caccatcaac agtgtaagcc cagcgggcat
     3541 cgaaggcatc aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg
     3601 cgacgcgaat atcaccatgc ggaatgtttt tctgttgcaa taccgccagc gcggtcatga
     3661 tttctgcaat acctgctttg tcatcggcac ctaacaaggt tttaccatcg gtggtaatca
     3721 gcgtctgacc cagtagctga tgcagcaccg ggaacataac cggtgataaa acttcatcgc
     3781 cgatacccag cgcaatatcg ccaccgcgat agttttcaac aatttgcgga ttcacatttt
     3841 tgccgctgca atccggtgag gtatccacat gagaaataaa gccaatcgcc gggatatcgc
     3901 cagggacgtt agccggtaac gtcgccatca aagtgccctt ctcacttaag gtcacattga
     3961 taagccccat ctcttcgagc tgctctttca gcagatgcaa taacttccat tggccttccg
     4021 tgctgggaac ctgtctcacc cctgcttttg attgggtatc cagagacacg tagttcaaaa
     4081 atcgctcaag tagtttatcc atgtagtcac cctcactttt tgtgacaaca ttattaagaa
     4141 gcaagaaaag acaaatattg cgtcaggtca cttttacccc tgcaagcggg aatatttatc
     4201 agcattacct taatgaatat aaagctaagc cagtaattca caacaaagat tggcgattca
     4261 agcggtttgc cgtagaatta ccgccctcat taagagtcac caaggtggtt aaccacaaac
     4321 cccgcatcgg taagccatcc gttgcgttta catgggacag agtaaaaaat tgaataaaca
     4381 accgagttcg ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa
     4441 agaggtcatt ccccagctgg atctgactat caacaatggc gagttcctca cgctgcttgg
     4501 cccttctggc tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg aaactgttga
     4561 ttccggacgc atcatgttgg ataacgagga catcacccac gttccggcgg aaaaccgcta
     4621 tgtgaacact gttttccaaa gctacgcact tttcccccac atgaccgtgt tcgaaaatgt
     4681 ggcctttggg ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga
     4741 agccctgcgg atggtgcagc tggaaacctt cgctcaacgt aaaccgcatc agctctctgg
     4801 tggtcaacag caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt
     4861 gctggatgag tcgctctcag cgctggatta caaactgcgt aagcaaatgc agaacgagct
     4921 gaaagcgtta cagcgtaagc ttggcattac attcgtcttc gtgactcacg atcaggaaga
     4981 agcactcacc atgtcagaca ggattgtggt gatgcgcgat ggtcgcattg agcaagacgg
     5041 cacgccgcgt gaaatctacg aagagccgaa aaacctgttt gttgccgggt tcattggcga
     5101 aatcaatatg tttaacgcca ctgtcatcga acgtctcgac gagcagcgcg tacgcgccaa
     5161 cgttgaaggc cgcgaatgta atatctacgt taacttcgcc gttgaaccgg ggcaaaaact
     5221 gcatgttctg ctgcgcccgg aagacttacg tgttgaagag attaacgacg acaaccacgc
     5281 tgaagggctg attggttacg ttcgcgagcg taactacaaa ggcatgacgc tggagtcggt
     5341 tgttgaactg gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg aagacgatcc
     5401 tgactttgac cactctctcg accaaaaaat ggccattaat tgggtagaaa gctgggaggt
     5461 cgtactggct gatgaagaac acaagtaagt tccagaatgt agtgattgtc actattgtcg
     5521 gttggcttgt gttgtttgtc tttctgccca acctgatgat cattggcacc agctttttgg
     5581 cccgcgacga cgccagtttt gtcaaaatgg tctttacgct ggataactac acacgtctgc
     5641 tcgatccgct ctattttgaa gtgctattgc actcgctgaa tatggcgctg atcgccaccc
     5701 tcgcctgcct ggtgctgggc tatccgtttg cctggtttct ggcgaagctg ccacacaagg
     5761 tgcgtccgct gctgctgttt ctgctgattg ttcccttctg gaccaactca ctgattcgca
     5821 tctacgggct gaaaattttc ctcagcacca aaggctatct caacgagttt ttgctctggc
     5881 tgggcgttat cgacacacca attcgtatca tgttcacgcc cagtgcggtg attatcggtc
     5941 tggtttacat tctgctaccg tttatggtga tgccgctgta ctccagtatc gaaaaactgg
     6001 ataagccgtt gcttgaggcg gcgcgcgatc tcggtgccag caagttacag acttttatcc
     6061 gcatcattat tccactgacg atgccgggga ttattgccgg atgtctgctg gtgatgctgc
     6121 cagcgatggg cctgttctat gtgtccgacc tgatgggcgg tgcgaaaaac ctgctgatcg
     6181 gtaacgtcat caaggtacag ttcctcaata ttcgtgactg gccgtttggt gcagccacca
     6241 gcattacgct gactatcgta atgggcctga tgttgctggt ttactggcgc gcttctcgtc
     6301 tgctgaataa gaaggcaaca gaaatagata attagtgctt tatattcaaa aggttaatag
     6361 gaaatttggc atgcttttta ctgtctagta aacagcatgc ctctatccat aaaatcaaac
     6421 agttgcaatc taattttgga gaacgttttt tcttctccct tttagcaaat cgctataaaa
     6481 aaacaacaaa tggtataaaa atgagcaatc ctaatccatg catgacggac tggagaaagt
     6541 attcgcaaaa gttaaaatca acagtctgtt ggttttgaga tataactctc ttttctaata
     6601 ttgacacctt ggctttgctg gttgttaaaa ttagcttgtt gattagtagt atggatagaa
     6661 cttactctgt tatttatttc taaaaatgga atttatcaat gcttcctaca tcgcaattac
     6721 gaccgaccgg gacattctgc tcctattccg ctgaaacatc agcagacatc aaaagcgaaa
     6781 tcacaccaat tcagatagaa gaagcgcggg ccagtggtcg tttatatatc aaagattgtg
     6841 atattgagta tctgccacag ttaccaaacg aaataacatc agttacaatc gaaaactgca
     6901 acaacctgac aacccttaca ggattgccgg ttaatacaca aaacctctcc gtcattaact
     6961 gtgaaaaatt acaaatcaca gacatgccat caaccgtaaa aaatctacat attgaattaa
     7021 ctgattcacc atttatacat ttcatatctg aaggcatcga gtgcctgacg gtttgccact
     7081 gctatatatc tggagtgcca gagagtgtcc gctaccttga aataaaaggt agcgccacag
     7141 acagcataaa aaatgttcca aacgggttat catctctcag catcaatagc tataacccgg
     7201 agaatcaggc cagaattgac aacctgatat caccgtcact gaagacgcta tcgctgactg
     7261 gatgtagcaa tattatactg ccggagaaac ttccggagag tgtgacatcg gtaaccattc
     7321 atgcggagca gaaaaccacg tggaacatcg gtgttgaagg gatgcctgat gggctggatc
     7381 ttgatttaca aaatgtacta ctctctccag atgtagttaa agcaaaaaac atcacctttc
     7441 agggcaacgc tctggatgtg gccttacact ttcgcgaggg agacattgtc tatggactat
     7501 cttcacccag agaaaaactt gtcaacagca ttaaactagt taacgacttt tccaaaaaag
     7561 atattataac tcagaatacg ttaacaaacg cagtatggga ccccagaaca cctcgcaaat
     7621 ataagcaaga tccacttatc aaaagagcat taaatgaaca cgaaagagga ataaaattta
     7681 aacaacactt aaagaatcac aataattata atgttaccat ggccgacctt tccgtataca
     7741 atcgcgacaa attatgggca aaaacaagca aggccggcct agagtttcag acattaacac
     7801 gcaataaaac ggttattttt tgtgcggatg agcttgtcaa ctcactcaaa ctcatagcta
     7861 acaagtcaga gggctatggc cagagtatta ccgccagcga attacgatgg atttaccgta
     7921 ataaagacaa caaccaaata atgaaaaaca taaaatttta tctacatggc aaagagatac
     7981 cagcagaaag aatattagat acaccagaat ggaaagacta tcgtccaaaa tactctggtt
     8041 ccacatataa atattcttaa tgataccaac ttataacgga atagcataaa aacacttttc
     8101 atggagcaaa ggagaaaaca atgccatttt caatcaaaaa cagattttca agttcacaag
     8161 tacattaccc ggaaatatcc ggtcccataa aagacaagcc agcgtcaaag aactgcatac
     8221 ttacatcaac aacatgtaat gtagatagct atacagtgta ccaaaaaaaa gcctgtagtt
     8281 ttgacatgcg cccacccggc gcaggagaaa gaaccccaaa actaaaactc tcagttactg
     8341 agatgacatg gctatctaaa actatagaaa cagagataca caacacaaaa gaatagcaac
     8401 cacttaccag agaacacaaa aagccacaga cccgaaacac ctgactgcaa gcaaccacct
     8461 ccacaggagg tggttttacc atgaacttcc tgtgtactga cttgtgttca taataatatt
     8521 tttgtgttta aactcaataa agtcacaaaa atgattgtaa tcatgcaatg tagtaaaatt
     8581 aaaatatt
//
LOCUS       DAZFKY010000186         8549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFKY010000186 DAZFKY010000000
VERSION     DAZFKY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..191)
                     /locus_tag="ACHKCL_003953"
     CDS             complement(<1..191)
                     /locus_tag="ACHKCL_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5764827.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENS"
     gene            complement(291..1142)
                     /locus_tag="ACHKCL_003954"
     CDS             complement(291..1142)
                     /locus_tag="ACHKCL_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFO5764828.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1569..6155)
                     /gene="ypjA"
                     /locus_tag="ACHKCL_003955"
     CDS             complement(1569..6155)
                     /gene="ypjA"
                     /locus_tag="ACHKCL_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5764829.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6220..6339)
                     /locus_tag="ACHKCL_003956"
     CDS             complement(6220..6339)
                     /locus_tag="ACHKCL_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5764830.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6563..6721)
                     /locus_tag="ACHKCL_003957"
                     /pseudo
     CDS             complement(6563..6721)
                     /locus_tag="ACHKCL_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7091..8440)
                     /locus_tag="ACHKCL_003958"
     CDS             complement(7091..8440)
                     /locus_tag="ACHKCL_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO5764831.1"
                     /translation="MLINKSNGFNASAVWGSGSYNENKSSKHMELLAHSIVKLICKEA
                     ASETYRGALEILQKIMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENVASEVHKSANKGEIEQLVEWSKRNE
                     QTLFDNIIKSDFHVGSLKPGSMNGVILEMPPNVCMEPRNSYENKIDEVSSLSESEEHP
                     IDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECCYTFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         2178 a         2079 c         1749 g         2543 t
ORIGIN      
        1 cttgaatttt cgatagttaa ttgaatccat tcgtctactt cactttcaat gaaagcaaca
       61 gagcgcgcac cgattttaac gggggaagga aaacgatttt ggttgatgag gtgatagatc
      121 caagctttac cgtatccagt tcgatgcaga acctctggta aacgaataag gcgggtgttg
      181 tgctgattca tagtcgtttc ctctgtgccg ttttgatgga aatgactata caaatgaatc
      241 agcaggaaaa agaccgaagc tgggaacgcc agagctaatg gaggttggtc ttagtcagaa
      301 tcagcgattt tcatcacgtc tgaaactctg atctttttca aatggctgtt cttgttgatg
      361 aacagattaa actggcgaat aaaaggtata ctcgccgcct tcaaagcaag aggtttgtct
      421 gtatccctga tttggtggtt caggcgagtg ttgatctcat catcgtcatc tgtatacaac
      481 aggcgagaaa ggcggtcttc tgatatacgt actttatata attttgacca taccagaatg
      541 tcgatcatgg gaaggagtct gtaattaatg attttcttaa tcgttccata accaaagcga
      601 atagcttccg ttacatctgg ttctatgcca cgcactttac gccattgagg aagtgcagcc
      661 tttagggatt cagcgatctc ttcatctgtc ccgtttgcta ggtcgatttc gaccatgaca
      721 gtatccctga acaattttgg caaaacttcc gatacagggc tatcgaggaa ttctctgtta
      781 acagaatact catcgcttcc atcccaaaaa aacagtgagt tccgtaatcc aactatgctg
      841 agttgagcca gtctttcggt tgtcgtcaaa aagaagtgcg ggggctggta aagagtatcc
      901 tctcttgtga gatagccgag atgcttttct gtgatgagaa agggctttcc agtgaagatc
      961 ctgtcgatgt agagccttac ggcttctgct tcaaattctt catgatatgg tttgaagaac
     1021 aatgtccttg ccagcaattc atggtagagg tgtataagcg gaagctcttc gaatccgcga
     1081 tatgtatcta tatccagcca cttcttcacc tctctcgtat gttcaactga ccagtttttc
     1141 ataacgatct ccaacaggca ttagacttaa tgtatgtagc aaacgtaatc gacatgttaa
     1201 aggaagacca atgaaatgag ttcagatgcc atcaggaagt tgaaccaaaa ggtttgatat
     1261 tttttgatac agttcgctaa cggactattt tcaggggttt atgccgttac gatttatggt
     1321 tcctttatag aaccaaaggt aaagtcagaa acagcagtta tctagaaaat tcaatcaaat
     1381 agatatctag ttacagggac gccagccaat catgattgga cggtgtaagg acaacaccaa
     1441 caaaaacagg aagttagaag tctcagcagg acaccgacca gacggtgagg agacataaaa
     1501 ggatacgcaa aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc
     1561 gttgatggtc agaacgacca gttcacacca gctaccgcgt tccacgggga ttccacaccg
     1621 gcaccatggc tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta
     1681 atacctgcct ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcatta
     1741 ttcaccttca cccggttatc atcgacaaat tctttgcgca cagccgcctt cagccacggc
     1801 tcaatttcca taccgttccc cagacgcatg ttgtaactca gcgttgcgcc cagttcacga
     1861 tatatactgc gggtatcgac tgatttcgat tccatgccat tggataaatg atattcgggg
     1921 ttatcagcgg tgaaccccgt taacgatgca tacggcgtca ggttccagtt accatcggta
     1981 aatcgcatcc cggtttcaat gtgaccgccc agcccgttgc tgtggtaact gccattggcg
     2041 gctccaccgc tgctcatttt accggctacg ttactttcaa aacggttcag cttcacgaca
     2101 ccgtccagat agaaaccact ttcatgttcc caactggcat agccgcccag agaataactg
     2161 cccacactgc catgtcctcc gcgatcaaaa ccgatatgtg aatgggaata acccataaaa
     2221 gcgcccagcg tcgcaatccc ctcaggaata tcattagggc tgtcgatccc cactgtcatt
     2281 ccggtcagcg tctgctcaaa cccggccccc gcatcggtgg tgacattatt acgggtgtta
     2341 tacgtcgccc cccagacatt attgttgtgt ggactcgctt tcattatgtt caaccgctcg
     2401 cgaatactgt ttagctcagc atcaaatacc aacggtaatg ttgctgccat attgagtacg
     2461 gctgcggtag aaggcgtgat gcgtttctcc ggaacgggtg tcggcgttgg ctcgggagtc
     2521 gggtctggtt tcggatccgg ttttgggtct ggttttggat ccggcttcgg atttgggttg
     2581 ggatttgggt tgggatccgg gttgggtttg acatcattgg tcaggttcca gttgctgttg
     2641 ccatcgcttt tcaggacata ctcataggtc ccaagatcaa cgaaaccgcc agtattgccc
     2701 agcgaaaacg aagcatcccc tccccctgtt ttcaccagcg tcatcgcgtc gtcagactga
     2761 ggactgacgc cggtatcctg aacaaagatt ttaaaattac cagtggcgtt gttgttgacg
     2821 accagttgat cgccccgaga gcctgcaacg ttggtatgca ggtagaaatt accgctaccg
     2881 gaaagttcat tggttgtcag cgtattgtat ataccgttag ttgtggcagg cgctgctgtt
     2941 tgtgctgata aataaacatt accgtcgtta agtagcaaac tgtttactct atactcgcag
     3001 ttgctggtgc ctgcacagga attattgctg ttaagccaga cactgccccg gctggcagcc
     3061 gtcaggtcgg caagcgtcgt atcaacgcca ttgccgatag ttaatgtcgc gctatcggta
     3121 atccggatcg ccccttcgag tttaactggc gtaacattat cccgtggcgt cattaacgac
     3181 aggcttcctg tcgcgccact gaccgatgca tccgccagcg tacctgcgta gacgattgct
     3241 gtcccgccag caacctggag atcttctgcc cgggccagag cctgaaactc atcttttgac
     3301 cgcccaaggg tatattgccc accagagtta acctttgtgg cggagtcctg accctggttt
     3361 tgcattgccc cccccttgcc aaccgtggag tcgcgagctt cggtacctgc taataccaat
     3421 aaattaccgc cattttccag caacatattg gtcgctaaat tgccggaaat ggaaaaagtg
     3481 ccgtactggt gagtaccgct gatttcaata ccgttagccg tgctcgtctg gagagcggca
     3541 ccgctgttct ggacgatatc tgtcgctttg ccattatcgt tgactgtcag cgtaccgcct
     3601 tcattgatcg ttgtttttat tgcctctccg ttagctgaaa ctgtttgtat tccgccgtcg
     3661 ttaattgttg tctcattcgc cacaccctcg acaatttgtt caccgccggt gagcgtcgtg
     3721 cctgtcgcag tggcttttgt tttgacgatc tcccgtccgc ccgtattgac ctgtgttttg
     3781 tcagaagagg tgtctgactc cacggttaac actccgccat ttgccagcag gatattgttc
     3841 gccgcaccct gctcgatgct gaacgcgacg ccatccgcgc gtgttcctgt gacccgcgtc
     3901 gccctggtgg ttgcaaccaa cgcgccctgg ctactctgct gtatccccgt tgcgctgcct
     3961 ttctcccgca catcgagtgt gccgccatca ttaagcaccg agttttcagc cagaccgccc
     4021 tcattaacta tctgtgaacc gccattaata atggaacctt ccgctgtccc gtttgccata
     4081 atttgttgta ggccagagac gatatcggta ttgatcgcct taccataatt ctgaacggtt
     4141 tgcgtgccac cattgatgtg tgttttctct gttgacccac catcaacaat ttgttcacca
     4201 ctttcgatat ttgcttcagt ggctaaacca tataccgttt gcttgccacc tttgatattt
     4261 gctttatcag aagtggcact ggcatatatt gtttgggtgc cagcactatt gagtacagtg
     4321 ccaacatctt ttccataaac atccattttg ccgttggcat taataatcgt gtcaactgcc
     4381 cgggaaccag tgacgactgt taatgagcca gcgttttcca gcactacatt tttagcttct
     4441 gaattcctga tgtagaaagc atcaccataa ctgttggttc cttcgataag tgttccggag
     4501 gtcgtggaag caattaatgc gccgccggat tgttgctcaa catgcttagc ctcaccaccg
     4561 tcctgaacct ccagaacgcc gccattatta agtctggtgc tatctgtttt agcctccttc
     4621 tggacaatca gcttaccgcc agcatcaatg gtagtatttt tcgccgaggt tttagccact
     4681 accgtcagtt cgccggtatt ttccagcaca acataattag cctcccctcc ggtaatagtg
     4741 aagtgagaga gcttgttgta tccttcgata tcagtccctg cacccgtgtt ggcaactaaa
     4801 gcactgcccg tctcctggtt aaccccatgt gcaataccgc cggtatagac aatcagcgag
     4861 cctccggcgc taatattgct gccaattgcc gtaccatctt tctcaacaac ctgccggctc
     4921 ccggaggata taattgttgt gtcagctttc ccgccgcttt tgatattttg cgttccgctg
     4981 ttgatattgg tgcctgtggc tatgccataa ttattaatat tctgtgtgcc accattaatt
     5041 atggtatttg tcgcgtttcc tgcaacatcc ataaccccgc cattatctat tcgggtcgca
     5101 tcagctttag cattggttaa aactgacatt gttcctttat ctttaataat cgtcttgttt
     5161 gccgaaccat atgcgtttat gtctaaatga ccaccgtttt ccagcaacac attgtctgcc
     5221 acgtgattgt ggatggagaa tgcaccttca ctattcgtac cgctcaccgt cgtaccgtta
     5281 gtgttagttt ttaaaattgc accatcgtgc tgggtaacat ttgttgccgt accaccacta
     5341 acatcaagca cgccaccgga ataaacttca ataacatccg aggagctggt gttatcaata
     5401 atttgcgtac caccagaata gatctgagta ttttttgccg ttgacttact attcagagac
     5461 tgagttccgc cttcaatcgt cgtgtccagc gcacggctct catatactct ttgctcaccg
     5521 ccatttttga tggtggttgt ttctactgtg ctctgttcaa catactgtcg accaccattt
     5581 atggttgtgt tcgttgccag acttccttgt actacgtcct gagagccaga cttatttatc
     5641 gttgtaccat cagcatgccc ttgaactttt actatctggc taccaccatc aatgagtgtt
     5701 ccattcgcac tccctccctc tacgcgtgaa gcaccgccct taattgtcgt tccattgctg
     5761 ataccccctt tataaacgtc ctgattgcca ctctcgatta tcgtacctgt ggaaataccc
     5821 ccgtcatgaa ttgactgtct gcccccgtta atggtggtat tattagactg ccccacataa
     5881 ttgttatgcc ttcctacatc ttgatatcca ccagattcaa taagacttcc attagatacc
     5941 ccgccataaa cattctgctg gccatggttg ataatatgag tgttatttgt tgtacctcgt
     6001 tcatctactc gttggctgcc atctacagtc tcatcgttta ccacaccaat aacatcagga
     6061 gtgaaggcag ccatcccggg cggggcatat atcaaggcag atatcaataa ggaaagtact
     6121 gagcgacgac aataataggg actggtcctg ttcataaatt tcatcctctg aaaagtgaat
     6181 actgagtagc gtttaagcga ccttagcttt gctgcaacat cagcccacag gcaccagacc
     6241 aggggattca tcctgaagag acagcgcaag tgtattgtgt tcaccgctca tcaaagacat
     6301 catgatgaaa tgatgatatt ccgcataaga atgaggcatt ttttaaacgc agtgcgctga
     6361 agtgtggttg gataaaaaag tcaatccatt caggaaatac gggcgtattc ttttctttcg
     6421 acaatgaggc cgttggcaaa ataaaatgat ttacataatc gtttctgatg aatatcttct
     6481 gctcacataa aaatcacaca ataactttga gatcgcagat tgttttactt ttacagcatt
     6541 cgtcccccca ttgttgggca aatatagatt gggccagagc acgaaagtta ataccacgtt
     6601 cgcacagctc ctccaacagc acgacaagat gccacatact gcgccccagt cggttcagtt
     6661 tacagactag cagtgctttc cctgccgatg atgtcctgag cagttttttc agtccggacc
     6721 tcctgccacg gttttacgtt gaagcggtaa ccctgagcac gcagttcttc agtcaggcgt
     6781 ggtgcaccgt aacgctgtta ttgctgggtg agatcaaaaa actttcaggc agctaaggaa
     6841 agttgaacca gacaatagat gaaatatttc aaccaattac agcaccaatt cagtcactgc
     6901 cagcccacca aataaatcaa ggggttacat gaaaacgtag cccctttttc tttggtagtg
     6961 acactaaaat ggatgtagtg tgaagaataa tcccgtttac tcaatcaata atacatattg
     7021 tttcaatcta cgttattatc tctttataaa aattgccatt tatttatcat tgaaaactgc
     7081 ttttagaact ttatacaaga ggactattca caacaggact attctcaatg ggagcatcct
     7141 caggggagtt atcatcaaag tcatcatcca taaataaaat atcatcgaat ggtgccacac
     7201 ccgtgatcat ttttattttt ttattacgat cagataagac tccatttaaa ccgttttcgc
     7261 ttacaggttt taatgatttt tcataactct catggttagc aggcgtatta aaaatacacg
     7321 gagtatcaac atcaaacaat gacattcccc agttcacata ttgaatatca tagttactga
     7381 agttctgtcc agaaaagaag catcccttaa aatccaatcc acgtagatta tataagccac
     7441 cctcttcttt ttggagagta atgttaattt tggctatctc ccggacatca tcgccatttt
     7501 tgtatttgaa taccgtttca agatgttctc cagacagctt gacttctgga aataatttga
     7561 aatcaaagcc tacattatta tgtgtcaacg tagatgaaca aaagatggaa aaagcttgca
     7621 gtgctgaatt atagctatcg attttatctt gaggctgcgc tcttggtaaa aacgtatagc
     7681 aacattcata aaaattaaac agaagctctg aagatcttcc atttttatca aataatatcc
     7741 ccttgaactc cttcacaaac gcatctgtta ttttttgaat atctatgggg tgttcctctg
     7801 actctgacaa agatgaaacc tcatctattt tgttttcata tgaattacgt ggttccatac
     7861 agacatttgg tggcatttct aaaataacac cattcatact acctggctta agtgaaccaa
     7921 catgaaaatc actttttatt atattatcga aaagggtttg ttcatttctt ttagaccatt
     7981 caactaattg ctcaatctca cctttattcg ctgatttatg aacttcagaa gccacatttt
     8041 ccttgatctc cggttttaat ttaatgagaa gattttccca gacctgcttg cttaaacata
     8101 ctacgtcagg ctcaccatca gtaactaaca cttcattatt atcaaagtgt acgttgaata
     8161 cctttaagtt attttcatca acatcatatt taatacgttt taattgttcc ccagctccca
     8221 taatgacaaa ggcgttgcct tcttgatata tacattcaga cattattttt tgtaaaattt
     8281 caagagcacc gcgatacgtc tctgatgcag cttccttaca aattaatttt acaatactat
     8341 gagctagtag ctccatgtgt ttagaagatt tattttcatt ataacttcca ctaccccaaa
     8401 ctgcgctagc gttaaatccg ttgcttttat taattaacat tttctattcc tcagttaatg
     8461 tctacatggc tatttttaat gttattattg tttgtcacta taaaaaatcg ctcatttgag
     8521 acaattgctg acattaactg cttcacttg
//
LOCUS       DAZFKY010000187         8464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFKY010000187 DAZFKY010000000
VERSION     DAZFKY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..439
                     /locus_tag="ACHKCL_003959"
     CDS             <1..439
                     /locus_tag="ACHKCL_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO5764832.1"
                     /translation="NNNFFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEI
                     EDNLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKS
                     RHEMLPVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            662..1159
                     /gene="ubiC"
                     /locus_tag="ACHKCL_003960"
     CDS             662..1159
                     /gene="ubiC"
                     /locus_tag="ACHKCL_003960"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5764833.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1172..2044
                     /gene="ubiA"
                     /locus_tag="ACHKCL_003961"
     CDS             1172..2044
                     /gene="ubiA"
                     /locus_tag="ACHKCL_003961"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5764834.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2199..4622)
                     /gene="plsB"
                     /locus_tag="ACHKCL_003962"
     CDS             complement(2199..4622)
                     /gene="plsB"
                     /locus_tag="ACHKCL_003962"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5764835.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4793..5161
                     /gene="dgkA"
                     /locus_tag="ACHKCL_003963"
     CDS             4793..5161
                     /gene="dgkA"
                     /locus_tag="ACHKCL_003963"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5764836.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5271..5879
                     /gene="lexA"
                     /locus_tag="ACHKCL_003964"
     CDS             5271..5879
                     /gene="lexA"
                     /locus_tag="ACHKCL_003964"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5764837.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5952..7277
                     /gene="dinF"
                     /locus_tag="ACHKCL_003965"
     CDS             5952..7277
                     /gene="dinF"
                     /locus_tag="ACHKCL_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5764838.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7393..7602
                     /gene="yjbJ"
                     /locus_tag="ACHKCL_003966"
     CDS             7393..7602
                     /gene="yjbJ"
                     /locus_tag="ACHKCL_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5764839.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7644..8159)
                     /gene="zur"
                     /locus_tag="ACHKCL_003967"
     CDS             complement(7644..8159)
                     /gene="zur"
                     /locus_tag="ACHKCL_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5764840.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1952 a         2047 c         2285 g         2180 t
ORIGIN      
        1 aaataacaat ttttttatcc aacttatcga tcaggctcta cgatcaacga atgatacgat
       61 caaagaaaat gcctggcatc tttataaaga gtggattcgt agtgatgatg tttcaccttt
      121 atttatagaa attgaagata atttaagaac ctttaacacg aatgaattaa cacgaaacga
      181 taatatcttt atcttgttct cctctgtcga tgatgggcca gttatggtgg taagctccca
      241 gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca cgtatatcta
      301 taaatccaga catgagatgt tgcctgttaa tcttactccg gaaacacttt tcggctccaa
      361 atcttatgat aaacatgcgc ttttccccat ttttactgcg agttggcgag ctaatcgtat
      421 aaagaataaa ggtatttaag taaaggaaaa catcaccgct cttggcatca tgggcggtga
      481 tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt
      541 ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac
      601 atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg
      661 catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattgtaaag agatccctgc
      721 cctggagccg caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga
      781 acagcaggga aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga
      841 aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt
      901 gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt
      961 aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt
     1021 cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg
     1081 gcgacgttcc cgcttgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc
     1141 ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct
     1201 gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg
     1261 gccaacatta tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc
     1321 ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc
     1381 tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc
     1441 ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt cgtttttact
     1501 ggtgctgacg ctgaatacga tgaccattct gttgtcgatt gccgcgctag cgctggcgtg
     1561 ggtgtacccg tttatgaagc ggtataccca tctaccgcaa gtggtgctgg gcgcggcgtt
     1621 tggctggtcg attccaatgg cttttgctgc tgtgagtgag tcggtgccat tgagttgctg
     1681 gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt
     1741 tgaccgtgat gatgatgtga agattggcat taaatccacc gcaatcctgt tcggccaata
     1801 cgataaattg attattggta ttttgcagat tggcgtactg gcgctgatgg cgattatcgg
     1861 tgagttaaat ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt
     1921 tgtttatcaa caaaaactga ttgccaaccg cgagcgcgaa gcctgcttta aagcatttat
     1981 gaataataac tatgttggtc tggtactgtt tttagggctg gcaatgagtt actggcattt
     2041 ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg
     2101 cgcttctcag gcctacacaa cagatcgcaa tttattgaat ttgcagatta tggaaggccg
     2161 gataaggcgt ttgcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca
     2221 ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc
     2281 atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc acgcagcgta
     2341 agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc
     2401 acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac
     2461 gggttggcgc tcaacagcca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct
     2521 gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc atcatcttgc
     2581 agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac
     2641 tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg
     2701 tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc
     2761 gaaggtagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc
     2821 tcgcgcggca gaatgatgat gtcgccgata gtgtctttct caacttcaaa cttgttcatt
     2881 tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag
     2941 taaggcacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg
     3001 gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc
     3061 gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt
     3121 aaccatgccg gacgcactgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc
     3181 tggttaaggt aggtcatcaa cggcattggt tcaccgaaat tgacgtaacc ctggccgaga
     3241 ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg
     3301 ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc
     3361 ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc
     3421 gtaccggttt tcggatccag caaacgcccc gtacgggaac gaccgccttc tacgaagtac
     3481 tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaatag
     3541 agcttattgc ctttaaaagt acggcgaata aagaacgcac ccagacggcg gaaaatcggc
     3601 ccggcaggcc agaaattcag gttgatcccg gcggcgatat gcggcggcac tagcccctgg
     3661 tggtacagca cgtaagaaag cagcaggtag tccatgtgac tgcggtggca aggcacatat
     3721 accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg
     3781 ccctggtaaa ggcggttcca ggtaaagccc agaatacggt cagtcaggcg gatcatctcg
     3841 taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca
     3901 tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg
     3961 agcagcttat taaacagatc ctgacgagca ggcagacgtg gtcctacggc agccagacgt
     4021 tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gcgcgatagt tttatccgtg
     4081 ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg
     4141 cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg
     4201 ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgacac tggcaccatc
     4261 tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata
     4321 gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag
     4381 cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa gtcatgtgcc
     4441 agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata
     4501 attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt
     4561 tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac
     4621 ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc
     4681 atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc
     4741 ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa
     4801 taataccact ggattcaccc gaataatcaa agctgctggc tattcctgga aaggtttacg
     4861 cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt
     4921 ggtcatcgcc tgctggctgg atgtggacgc gattacccgc gtgctgctta tcagctccgt
     4981 gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat
     5041 tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct
     5101 gattgcgatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata
     5161 acccttccag aattcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc
     5221 ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt
     5281 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
     5341 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
     5401 aagaacatct gaaggcgctg gcacgcaaag gcgtaattga aattgtttcc ggcgcatcac
     5461 gcgggattcg tctgttgcag gaagaggaag aagggttgcc actggtaggt cgtgtggctg
     5521 ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct
     5581 tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg
     5641 gcattatgga tggcgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg
     5701 tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata
     5761 aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc
     5821 agagcttcac cattgaaggg ctggcggttg gcgttattcg caacggcgac tggctgtaac
     5881 atatctctga gaccgcgatg ccgcttggcg tcgcggtttg tttttcatct ctcttcatca
     5941 ggcttgtctg catggcattc ctcacttcat ctgataaagc actttggcat ctcgccttac
     6001 ccatgatttt ctccaatatc actgttccgt tgctgggact ggtcgatacg gcggttattg
     6061 gtcatcttga tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct
     6121 ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt
     6181 atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaacctttg ctgttggcgt
     6241 tgggggctgg ggcgttaatt gcgctgctgc gtacgccgat tatcgatctg gcgctgcata
     6301 ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt
     6361 taagcgcacc ggcgtcgctg gcgaatctgg tattacttgg ttggttactc ggcgtgcaat
     6421 atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg
     6481 tctggctggt gatgggactg catatgaacg tgcggggcgc ggcgctggcg acggttattg
     6541 cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg
     6601 gaatctccgg cgaaatgctg aaaacttcct ggcgaggaaa cttccgtcgc ttgctggcac
     6661 ttaaccgcga tatcatgctg cgctcgctgt tgttgcaact ctgtttcggc gcgatcaccg
     6721 tacttggcgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg atgacgctac
     6781 tcacctttac cgcctatgcg ctggacggtt ttgcctacgc ggttgaagca cattccggtc
     6841 aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcatgccgcc
     6901 agtcggggat cgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca
     6961 ttgcgttact ggcgtcgtta acccagattc agcagctggc cgaccgctat cttatctggc
     7021 aggtgatttt gccgttggtc ggcgtctggt gttatctgct ggacggcatg tttataggcg
     7081 caacgcgtgc tgctgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga
     7141 cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gttggcgtta accgtctttc
     7201 tggcgttgcg cgggctttct ctggcagcta tctggcggcg tcactggcgc aacggtacct
     7261 ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc aaattgctga
     7321 ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat
     7381 gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa
     7441 gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt
     7501 gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa
     7561 gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga
     7621 cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca
     7681 tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca
     7741 tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcatg
     7801 atgtcttcca cgccttcagc acattcttct ttcaccgcgc cgcagcgatc gcaaataaac
     7861 atagctgacg tatgggtggg ctggtcgaac agatggcaga gcacataact gttggtggat
     7921 tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc
     7981 ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca gatcataagc gctgatagcg
     8041 ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta
     8101 cgctgcgcgc agattttgtc agcctgcgct aataactcct gcgttgtggt cttttccatc
     8161 gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc
     8221 gagaaccctt attgttgccg taatgttgct tttctgtttt gtaggtaaga tgttgtgttg
     8281 cctggcagta ccattatcaa cacgataata attaataatt catttttaaa taaattgctt
     8341 caagttaact cgctatttat ttaaccgtgg ctaatcgtat acccgaattt cagaaaatta
     8401 tgagcgattt ttgatagtta ccggttccaa aaacacttat aaggaggaca cggtacgaca
     8461 aaaa
//
LOCUS       DAZFKY010000188         8398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFKY010000188 DAZFKY010000000
VERSION     DAZFKY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1668
                     /gene="espL4"
                     /locus_tag="ACHKCL_003968"
     CDS             <1..1668
                     /gene="espL4"
                     /locus_tag="ACHKCL_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO5764841.1"
                     /translation="PHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFG
                     LVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHYVVSVYDPNVTNDK
                     IRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGTVD
                     EITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAIN
                     YGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSI
                     LNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALSLLIRT
                     CHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVD
                     LLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEY
                     PGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELACELG
                     HRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1637..3373)
                     /gene="aceK"
                     /locus_tag="ACHKCL_003969"
     CDS             complement(1637..3373)
                     /gene="aceK"
                     /locus_tag="ACHKCL_003969"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5764842.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3557..4861)
                     /gene="aceA"
                     /locus_tag="ACHKCL_003970"
     CDS             complement(3557..4861)
                     /gene="aceA"
                     /locus_tag="ACHKCL_003970"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5764843.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRRYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4891..6492)
                     /gene="aceB"
                     /locus_tag="ACHKCL_003971"
     CDS             complement(4891..6492)
                     /gene="aceB"
                     /locus_tag="ACHKCL_003971"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5764844.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6761..7690)
                     /gene="metA"
                     /locus_tag="ACHKCL_003972"
     CDS             complement(6761..7690)
                     /gene="metA"
                     /locus_tag="ACHKCL_003972"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5764845.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7847..8290
                     /locus_tag="ACHKCL_003973"
     CDS             7847..8290
                     /locus_tag="ACHKCL_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5764846.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
BASE COUNT         2129 a         2064 c         2075 g         2130 t
ORIGIN      
        1 ccccatcaag atgattactc ttcagctgaa aaatttctca ctcacttcaa caaaaaatgc
       61 aaaaaccaga ctttggcgtt gatttccagc cgtcctgagg ggcgttgcgt tgctgcctgc
      121 ggtgatttcg ggctagttat gaaggcatat tttgacaaga tggaatcaaa tgacctcagt
      181 gttatggctg ccatattatt ggtagataac catgctttga cggtccggct aagaataaag
      241 aacacaactg aaggatgtat ccattacgtg gtttcggttt atgatcctaa tgtaactaac
      301 gataaaataa gaatcatgag cgaaagtaaa gaggatatta aacactattc actgatggat
      361 tttatgaatg tagattatag cctcctgaaa tggtcaaatg atcatgttat taaccaatct
      421 gttgcaataa ttccagcact tccaaaagaa cagctattga tgctaaaagg aactgtggat
      481 gaaataaccc ctccattatc acctgcaacg atgaatttgc taatggcaat tggtcagaat
      541 caccaactta agcaactgat gattcaactc cagaaaatgc cagaactaca tagaacagaa
      601 atgttgactg cctataatag tattaacctc cccggtttat atttggctat aaattatggt
      661 aatgcggata tcgttgagac tatttttaat tcattgtcag agccaggata tgaaggatta
      721 ctctcgaaaa aaaatctcat gcatattctg gaggcaaaag ataaaaatgg tttttctgga
      781 ttatttttag cgatatcacg taaggataaa aatgttgtaa cctcgattct gaacgcctta
      841 cctaaactgg ccgcaacaca tcatttagat aacgaacaag tgtataaatt cctgagtgcc
      901 aaaaatagta cgtccagcca tgttttatac catgttatgg cgaatggtga tgccgacatg
      961 ctgaaaattg ttttggacgc gttatctttg ttaattcgca catgtcattt gactaaagaa
     1021 caggtactcg atctcctgaa ggcaaaggat ttttatggtt gcccaggact atacctggcg
     1081 atgcaaaatg gacatagcga tatcgtgaaa gttattcttg aagcattgcc cagcctggcc
     1141 caggaaatta acatttcagc ttccgatatt gtagatcttc tgaccgctaa aagtcttgcg
     1201 cgcgacacgg gtttgtttat ggccatgcag cgcggacata tgaacgttat taatactatt
     1261 tttaacgcat tacccactct gtttaatacg tttaaattcg ataaaaaaaa tatgaagccc
     1321 ctcctcctgg caaataattc taatgaatac ccaggtttgt tttcagcgat acagcataaa
     1381 cagcaaaacg ttgtagagat ggtttatctt gctttatctg accatgcacg cctgtttgga
     1441 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
     1501 tttgagttgg cttgtgaatt gggtcaccgg gttattgctg aattaatctt taatacatta
     1561 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
     1621 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
     1681 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
     1741 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
     1801 cggcgcatag ccaatggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
     1861 acggttcgct ggcaagttcg tcttccgggt agcgcggcgg cgggatatcg cggaaattca
     1921 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
     1981 caccgaagtt tttaaacagc atgtcgcccg ggaaaatgtt agcagcggca agctggcgaa
     2041 tggcattgcc gtactcttca atggcgtcgc gcagctgctg accttccact tgttccagcc
     2101 agatattgag cggcaccatc cgccgctcaa tataaagatg gcgaatcaca atttgttcgc
     2161 cgagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat gccggggaaa
     2221 tatgccgctt ctccagcaca aagttttcaa actcctgggt atccgccatt cggcccacgc
     2281 gatcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
     2341 gcggcgcgaa cttgtctttg atgactttaa atacacggtc aaagcccggc agcgtgaaca
     2401 ccaacatcac catgccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
     2461 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
     2521 tatacaattc agcggtggtt ttacctggca gaatttcccg taaccactcg accagcgctg
     2581 cgggcagcgg cgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
     2641 cggcggtcgt cgtcagacag gtatcaataa acagttcgcc gtcatccgtc tggtggatcg
     2701 gcaataaaaa tggcaatgtg ccggaaggcg ttattagttt gcccaccagc caggcggctt
     2761 tattacggta aaacagttcg ttcgccacct gtaaatgact tttggagagg ttttctggcc
     2821 ccaacgtttc cgtcagatgg cgaataatgt aatggatgtc acggctttta ttctgccagt
     2881 gcagacgcaa cggcaggtcg ctgataacgc gcatcagcag agattcccag ccgtgatcgg
     2941 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgggagc
     3001 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
     3061 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgggtgtaat
     3121 gctctttgac gcgcagtaaa aattccgcgt ccgtgctttg gccgttggta atgcagcgca
     3181 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
     3241 gctggacagc atgccagtcg gcttgttcga aacgctgctg cgccccggaa gtcacttcga
     3301 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
     3361 ggccacgcgg catttagcgc cctcatcagg agcagagaat tgccggatgc ggcgtaaacg
     3421 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
     3481 caggcatcgt gcaccgattg ccggatgcgg cgtatatgcc ttatccggcc tacagtcagc
     3541 aacgtttgta ggttgcttag aactgcgatt cttcggtgga gccagtcagc gcggtaaccg
     3601 acgacgtgcc gccctgaata atggtcgtca ctttatcgaa gtaacctgta cccacttcct
     3661 gctggtgaga tacgaaggtg tagccatctt tcgcggcggc gaattccggc tgctgcactt
     3721 tctcaacata gtgcttcata ccctcgccct gggcataggc gtttgccaga tcaaacatgt
     3781 tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
     3841 acagctgctg ctggaagctg gcaatagtgt tgtcgtcgag gtttttctgc cagttgaacg
     3901 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttccgcaa
     3961 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccataccagg tcagcatatg
     4021 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
     4081 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tcggaggtga
     4141 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
     4201 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc accaaaactt
     4261 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
     4321 ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
     4381 cggcttccgc gtcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
     4441 caatgcccgc ggaccattgg atctgatccg cacggcggaa agtgttgttg atccgctcca
     4501 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
     4561 ggttagcgtc cgccgctacc tgccatcccg acagatagac cgcttcaata cccgctttcg
     4621 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
     4681 actcgccatg cagcagacgc cacattttcg ccgcgcccag ttgcgccagc gtgcattcag
     4741 gattgactga accgcgtaat ttcaccacat cttccgcgct gtatcggcga gtaattcctt
     4801 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
     4861 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
     4921 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
     4981 aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
     5041 gcccagcatc tggcggaaca aggctttagt taccggtttg ccattgctca acgttttttg
     5101 atgatggatc cactgccaga ttgaggtacg ggaaatttca gccgtcgccg catcttccat
     5161 caggccataa atcggcacgc agccgttgcc ggagatccat gcttcgatgt actgcacagc
     5221 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
     5281 ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat ttttacggga
     5341 gccgagaatg tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
     5401 tgtgccatcg tgaccgttat tggcttccag cgatttatcc gcttttactt tgttgagcac
     5461 ctggttattg tgctcttcat ctttgctcgg aataaacgcc gccatgccgc ccatcgcaaa
     5521 agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca ggaatggttt
     5581 atccatcgtt actgcctgtc tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt
     5641 gatatagctg aagatgtaat cccaacgacc gcagttcaga ccaacaatat ggtcacgcag
     5701 cgcgtgaagg atttcatcca tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc
     5761 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
     5821 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cactgccctt
     5881 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctgccggg
     5941 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
     6001 aatcaaaacc gctggattgg gcttgagctg gtaaattttg cctgcttcat tggtgtaact
     6061 gatggtgccg ttaaccgcat cacgcaggtt aatttgcccg tcgatcactt tgttccagtc
     6121 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tgagcgcgtt
     6181 gatcaccatc ttgcgctcta cggatccggt aatctctacg cggcgatctt ctaagtccgc
     6241 aggaatcccg cgaattttcc agtcagcatc acgaatggaa gctgtttccg aaataaaatc
     6301 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
     6361 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
     6421 aatttgcttc tcctgctcgc catacggcct tatgaaagcc agttcatcgg ttgttgttgc
     6481 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa
     6541 ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa atcaaaatca
     6601 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tattaataaa
     6661 atttattcac ctactgaggt gtgtttcgga aataaaaaag gcacccgaag gtgcctgagg
     6721 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
     6781 tgtgccgtag atcgtatggc gtgatctggt agacgtaata gttgagccag ttggtaaaca
     6841 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatca ttgtgcggga
     6901 aatagttata cggtacttcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
     6961 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttac
     7021 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
     7081 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccaag aatgaatcat
     7141 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
     7201 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgct gcctgtaccg
     7261 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
     7321 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
     7381 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
     7441 gctctgcggg cgtgttacgc gattcacggg aatcgatgcg caacagctga atatcgacct
     7501 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
     7561 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
     7621 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
     7681 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
     7741 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
     7801 aggaaaagac tacgttagaa tataagaatg actaaaagga gagaaaatgg ttattagtat
     7861 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
     7921 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggttcgttc
     7981 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggatcagc cggttgggtt
     8041 tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgcgcggctg
     8101 cggcgtgggc cggatgctgg tgaagcatgc gctctcaatg gccccggaac tgacaaccaa
     8161 tgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
     8221 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctggatc tggcgtatgt
     8281 gggggaataa gctgccccct atcgtgcctg cgtaagctgg ataaaacgcg ccagcgtcgc
     8341 atccggcatt tttttgcacc aacgcaaaaa ggccatccgt caggatggcc tttcgctt
//
LOCUS       DAZFKY010000189         8309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFKY010000189 DAZFKY010000000
VERSION     DAZFKY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(6..212)
                     /locus_tag="ACHKCL_003974"
     CDS             complement(6..212)
                     /locus_tag="ACHKCL_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5764847.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            525..614
                     /gene="kdpF"
                     /locus_tag="ACHKCL_003975"
     CDS             525..614
                     /gene="kdpF"
                     /locus_tag="ACHKCL_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5764848.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            614..2287
                     /gene="kdpA"
                     /locus_tag="ACHKCL_003976"
     CDS             614..2287
                     /gene="kdpA"
                     /locus_tag="ACHKCL_003976"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5764849.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHKCL_003977"
     CDS             2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHKCL_003977"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5764850.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHKCL_003978"
     CDS             4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHKCL_003978"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5764851.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHKCL_003979"
     CDS             4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHKCL_003979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5764852.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHKCL_003980"
     CDS             7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHKCL_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5764853.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1736 a         2165 c         2427 g         1981 t
ORIGIN      
        1 agcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccaaac cggtttatcg
       61 ctggttttcg accagacgcg atgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      121 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat aagtacggcg taaaaagata
      181 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      241 gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      301 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcaccc
      361 tgtaattaag ttacacaaaa gttaaattaa tactaaacat tagttaaatc atggcttttg
      421 ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt tgcagccaga
      481 attctaccct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      541 tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc ctgatccatg
      601 cggaggcatt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      661 aatggtgctg gcgcgtccct taggtagcgg gctagcgtgg ctgattaatg atattcctct
      721 tcctggtacg acgggcgttg aacgcgtact ctttagcgcg cttggcgtct ctaaccatga
      781 gatgaactgg aagcagtatc tctgtgccat tctcggcctg aacatgctgg ggctggcggt
      841 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      901 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      961 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcgggct taacggtgca
     1021 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccctcattc gtgcatttac
     1081 ccgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
     1141 ggtgctcgtc cctgtggcgt tggtgatcgc actgtttttt attcaacaag gtgcgctgca
     1201 aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc tgttaccaat
     1261 gggtccggtg gcttctcagg aagcgatcaa gatgctcggc actaacggcg gtggtttctt
     1321 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
     1381 gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tgacgggcga
     1441 tcgccgtcag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1501 cgtggtgatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1561 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1621 ggtcgtgact acggcggcgt cctgtggcgc ggtgattgca atgcatgatt cgtttaccgc
     1681 tctcggtggc atggtgccga tgtggctgat gcaaattggt gaagtggtat tcggcggtgt
     1741 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1801 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgtg agatgaaact
     1861 gactgcactg gcgattctgg tcaccccgac gctggtgctg atgggcgcgg cgctggcaat
     1921 gatgaccgac gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt
     1981 gctgtacgcc gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     2041 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     2101 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     2161 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     2221 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     2281 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     2341 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     2401 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2461 tcgcgatggc aagcggcgcg atgcctggca atgcgctgtt tagcgtggcc attagcggct
     2521 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2581 aagcgcaggc caacagcctg aaaggggtga aaaaaacggc ctttgcccgc aagctgcgtg
     2641 agccgaaata tggcgctgcg gcggacaaag ttcccgccga tcaactgcgt aaaggcgata
     2701 tcgtactggt agaagcaggc gatattatcc cctgcgatgg tgaagttatt gaaggcggcg
     2761 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2821 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2881 gtagcgttaa ccccggcgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2941 agcgacgcaa aacgccgaac gagattgccc tgaccatcct gctgattgcc ctgactatcg
     3001 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgcagtca
     3061 gcgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     3121 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg gttgccacca
     3181 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctgctggat aaaaccggca
     3241 ccatcacact cggtaaccgt caggcatcgg agtttatccc tgcgcagggc gtggatgaaa
     3301 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggccgca
     3361 gtattgtgat cctcgccaag cagcgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3421 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaat atcgacaacc
     3481 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggtggtc
     3541 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg gccacaccgc
     3601 tggtagtagt agaaggttct cgtgtgctgg gcgttattgc gttgaaagat atcgtcaaag
     3661 gcggcattaa agaacgcttc gcccaactgc gcaaaatggg tattaaaacg gtgatgatta
     3721 ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggcgtc gatgattttc
     3781 tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag gcggaaggcc
     3841 gtttggtagc aatgaccggc gacggcacca acgatgctcc ggcgctggcg caggcagatg
     3901 tcgcggtggc gatgaactcc ggtactcagg cggcgaaaga agcgggcaat atggtggatc
     3961 tcgactctaa cccgaccaag ttgatcgagg tggtacatat tggcaaacag atgctgatga
     4021 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     4081 ttccggcggc attcgcggta acgtatccac agttaaatgc gctgaacatc atgcgcctgc
     4141 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     4201 tgattcccct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     4261 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     4321 tcattgattt actactgacc gtttgcggtc tggtgtgagg tttaccatgc gtggattacg
     4381 tccggcatta tcgacattta tctttctgtt attgattact ggcggtgttt acccgctgct
     4441 gaccaccgcg ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga
     4501 aggtgatacg gtgcggggtt cggcattaat cgggcagaat tttaccgaca acggctattt
     4561 tcatggtcgc ccgtcggcaa cggcagaaat gccttataat ccacaggctt ctggcgggag
     4621 caatctggcg gtcagtaacc ctgagctgga taaactaata gccgcacgcg ttgctgcatt
     4681 acgggccgct aacccgaatg ccagcacgag cgttccggtt gaactggtga cggcgtcggc
     4741 aagcgggctg gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcgtagc
     4801 gaaagcgcgt aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca
     4861 accactggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcact
     4921 agataaactt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4981 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgcgca ggcgtcggga
     5041 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     5101 ttggcgtggt agaaacccac gggcggaaag ataccgccgc catgctggaa gggctggctg
     5161 ttctgccgcc aaaacgcctg gcgtaccgtg ggcggcacat cagcgagttt gatctcgatg
     5221 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     5281 caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa gctggcattg
     5341 atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     5401 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5461 tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5521 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5581 ccctgcgcaa actggcactg cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct
     5641 ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca
     5701 tcggccataa caccggcagc gaaaaactgg tccgcgcggc ggcgcggctg gcgtcacggc
     5761 tggggagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga
     5821 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5881 caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt gaacataatc
     5941 tcggcaagat tattctcggt cgcccagcct cgcgccgctg gtggcgtcgg gaaacgtttg
     6001 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     6061 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     6121 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     6181 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatcta cttggcgtgg
     6241 tggtggtggc gctattttat ggacgctggc cttcggtggt tgctaccgtc attaatgtgg
     6301 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     6361 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgcgg
     6421 gtgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6481 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6541 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6601 aattgcagcc gttaacacat ccgcaaggaa tgacgccgtg ggacgatgcc atcgcgcagt
     6661 ggagttttga taaaggcctg cctgcgggtg cgggcaccga cacgttaccc ggcgtgccgt
     6721 accagatttt gccgttaaaa agcggtgaga aaacctacgg gctggtggtg gtggaaccgg
     6781 ggaatctgcg tcagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt
     6841 tagtcgccaa tgcgcttgag cggctgacgc tgaccgccag cgaagaacag gcgcggatgg
     6901 caagcgaacg tgaacagatc cgcaacgccc tgctggcggc gctttcgcat gatttacgca
     6961 ctccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     7021 gatcacccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     7081 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     7141 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     7201 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     7261 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     7321 ccgaaattgg tatcgatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata
     7381 acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7441 ataaagagtc ggcagtaccg ggggtagggc ttggactggc aatttgtcgg gcgatagtgg
     7501 atgtacacgg gggcactatt accgcgttca accggccgga aggtggtgcc tgttttcgtg
     7561 ttacacttcc ccagcaaact gcccctgaac ttgaagaatt tcatgaggat atgtgacaaa
     7621 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7681 cgacgggatg cgcgtctatg aggccgaaac gctgcaacgc ggcttgctgg aagcggctac
     7741 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7801 tatccgcgac ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga
     7861 agagagcgac aaaatcgccg cgctggatgc cggagcggat gattatctaa gtaagccgtt
     7921 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7981 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     8041 tcaccggggt gaggaagagg tgcatctcac cccgattgag tttcgcctgc tggcagtgct
     8101 gctcaacaac gccggaaaag tgctcaccca gcgccaactc cttaaccagg tgtgggggcc
     8161 aaacgcggtc gaacacagtc attatttgcg tatttatatg ggacatctgc gacaaaaact
     8221 ggaacaggat cccgctcgcc ccagccattt cattactgaa accggtattg gttatcggtt
     8281 tatgctttga atttttattc aataaattc
//
LOCUS       DAZFKY010000190         8229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFKY010000190 DAZFKY010000000
VERSION     DAZFKY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(153..1043)
                     /gene="malG"
                     /locus_tag="ACHKCL_003981"
     CDS             complement(153..1043)
                     /gene="malG"
                     /locus_tag="ACHKCL_003981"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5764854.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1058..2602)
                     /gene="malF"
                     /locus_tag="ACHKCL_003982"
     CDS             complement(1058..2602)
                     /gene="malF"
                     /locus_tag="ACHKCL_003982"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5764855.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2756..3946)
                     /gene="malE"
                     /locus_tag="ACHKCL_003983"
     CDS             complement(2756..3946)
                     /gene="malE"
                     /locus_tag="ACHKCL_003983"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5764856.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4311..5426
                     /gene="malK"
                     /locus_tag="ACHKCL_003984"
     CDS             4311..5426
                     /gene="malK"
                     /locus_tag="ACHKCL_003984"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5764857.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5498..6838
                     /gene="lamB"
                     /locus_tag="ACHKCL_003985"
     CDS             5498..6838
                     /gene="lamB"
                     /locus_tag="ACHKCL_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5764858.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            6989..7909
                     /gene="malM"
                     /locus_tag="ACHKCL_003986"
     CDS             6989..7909
                     /gene="malM"
                     /locus_tag="ACHKCL_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5764859.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8138..>8229
                     /locus_tag="ACHKCL_003987"
     CDS             8138..>8229
                     /locus_tag="ACHKCL_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764860.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLL"
BASE COUNT         2017 a         2146 c         2135 g         1931 t
ORIGIN      
        1 taataagctc tatgatggaa atattaaccg gcgaacgatt cagattgcag acgaaagaaa
       61 aaaaggcgct ccgtggagcg ccgaataaca gtcacaagtt gggataacgt aagttgaggg
      121 tgcagcggca taacattggc agaacaacat ctttaacctt tcacaccacc tgccgtcagg
      181 ccgttgacca gccagcgttg agccagcaag aagacgatgg tgatcggtaa tgcagacatc
      241 acggcagcgg cggcaaagtc accccacagg tagttttgcg gattgaggta ttgctgcatc
      301 cccacggcca gggtgtagct gtttacgtca cgcagtaaca gcgacgcgac cggaacttca
      361 gtaatggcag cgataaacga caggatgaat accaccgcca gaatcggtac tgacagcggc
      421 aacaggacaa ggcggaaggc ctgccacggt gtcgcaccat ccagcgcagc cgcttcttcc
      481 agcgaactgt cgatggtttc gaaatagcct ttgatggtcc agacgtgcag cgcaatccca
      541 cccagatacg cgaaaattac gccgccgtga gtattcaggc caatgaatgg aatgtactca
      601 cccagacgat caaacaacgc atacaacgcg accagtgaaa gtactgccgg gaacatctgg
      661 aaaatcagca ttcctttcag cagcgtcgct ttgcctggaa agcgcatacg ggcgaaagcg
      721 taggcgcagg tggtggagag cgccacaatg ccaatcgcgg aaatcccggc gacctttacc
      781 gagttccaca gccacagcag caccgggaat ggcggtggcg taatgcgacc atcagcctgt
      841 tccacgctaa aacctaatgc cagtttccag tgatcccagg agatttgctc cgggatcagg
      901 ctgccggtcg caaagttccc ctggcgcagt gagatagcga cgaccatcag cagcgggaac
      961 ataatcgccg cgataaaaag tagcagtagc aggtgagtaa taaacaaacg tgctttttgc
     1021 gatttcggtt ggaccattgc catttttgtt atctccctta atcaaacttc attcgcgtgg
     1081 ctttcaggtt cactatcgcc agcgcaccca ccagcaggaa gatcagcgtg gcaattgctg
     1141 ctgccagacc gaagtcctga cccccgccgc cttcaaaagc gatgcggtag gtgtagttaa
     1201 caagcaggtc ggtataaccg gctggcgtgg tcgtgccaag acgatccggg ccgccgttgg
     1261 ttaacagttg aatcagcacg aagttgttaa agttaaaggc gaagctggcg atcatcagcg
     1321 gcgtcagcgg tttaatcagc agcggcagcg taatcttaaa gaagttctgg aacggacctg
     1381 cgccatccat tgctgaggct tcatacaaat cgtccggaat cgctttcagc aagcccatgc
     1441 agaggatcat catgtacgga tagcccagcc aggtattgac gataatcagc atcgttcggg
     1501 cggtggtcgg atcgctgaac caggcgggct tcacgccaaa cagcgcgctc aacatcatgt
     1561 tgatctcacc gaagctctgg ttaaacaacc ctttgaaaat caagattgaa atgaacgatg
     1621 gcaccgcgta gggcagaatc agcaggacgc gatagaccgc tttgccgcgc aacgcttccc
     1681 actgcaccag acacgccaga accatgccga ctgccaccgt taaaaagaca gtgatcagcg
     1741 agaacaccac ggtccagacg aaaatggcga ggaacggttt ctgaatgcct tcgtcggtga
     1801 agacgcgagt aaagtttttc cagccggtgg tcacggtgta accggggctt agcttttcat
     1861 caccccagtt gccgtcggcg gtaatggact ggtaaaagcc aatttggtta ttcggacgat
     1921 atttcacgcc gctctgatta ttcgtcaacg tgccgtcacc gtcgagtgtg tagagcggct
     1981 gcgtgccaga aaactggcgc agggagctca tcatcacttt gttgccatcc ggcagaatgg
     2041 cggtaatgtc actcagcgcc tgacgattct gggtaatcac gcgtagattt gcgcgttcgc
     2101 cttcgggctg ggcggtcgtt tctttcagtt gcagtttttg ctctctgcca aatttaaaag
     2161 cgtcggagag gtaatttttg ccggtttcgc cgtcgctgag cgccagttgc cactcatcgc
     2221 ccgccgggta aagaccaaag ttataggttt tgcctgcttg ccaggagcga tctaacaaca
     2281 cttcctgtgc acgttcaaaa gtcagctggt tagtgctgct gtagttggtg aaggcaatgg
     2341 cgatggtgca gaccagaggg aagaggacga ataatcccat tccagccatt cccgggtaaa
     2401 catagcgcca ggcgtaggct ttacgattgg cgaaaatata cagccccgct gaactcaata
     2461 tcagcgtggt aatggcgaac aggtattccc cttgtgcgta cattaaaaca acaaggtaac
     2521 ccaccagcag gccgagcaga cctaacactg accatttcag cgcgtcgctt tgccaccaat
     2581 gtttcttttt aatgacatcc atggggttct tcctcattcc aggacggata aggctttcat
     2641 gccttatccg acaacaactg cctgatgcga cgctgacgcg tcttatcagg cctacatacg
     2701 tttcggtttt gtaggccgga caaggcgttc acgccgcatc cggcatttca cagcattact
     2761 tggtgatacg agtctgcgcg tctttcaggg cttcatcgac agtctgacga ccgctggcgg
     2821 cgttgatcac cgcagtacgc acggcatacc agaaagcgga catctgcggg atgttcggca
     2881 tgatttcacc tttctgggcg ttttccatgg tggctgcaat acgtggatct ttcgccaact
     2941 cttcctcgta agacttcagc gctacggcac ccagcggttt gtctttatta accgcttcca
     3001 gaccttcgtc agtcagcaga tagttttcga ggaactcttt cgccagctct ttgttcggac
     3061 tggcggcgtt aataccggcg ctcagcacgc caacgaacgg tttagatggt tgacccttga
     3121 aggtcggcag taccgttaca ccataattca ctttgctggt gtcgatgttg gaccatgccc
     3181 acgggccgtt gatagtcatc gctgtttcgc ctttattaaa ggcagcttct gcgatggagt
     3241 aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc aaccaggaag gtcagacccg
     3301 ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc gtacttgccg ttttcatact
     3361 tgaacgcata acccccgtca gcagcaatca gcggccaggt gaagtacggt tcttgcaggt
     3421 tgaacatcag cgcgctctta cctttcgctt tcagttcttt atccagcgcc gggatctctt
     3481 cccaggtttt tggcgggttc ggtagcagat ctttgttata aatcagcgat aacgcttcaa
     3541 cagcgatcgg gtaagcaatc agcttgccgt tgtaacgtac ggcatcccag gtaaacggat
     3601 acagcttgtc ctggaacgct ttgtccgggg tgatttcagc caacaggcca gattgagcgt
     3661 agccaccaaa gcggtcgtgc gcccagaaga taatgtcagg gccatcgcca gttgccgcaa
     3721 cctgagggaa tttctcttcc agtttatccg gatgctcaac ggtgacttta attccggtat
     3781 ctttctcgaa tttcttaccg acttcagcga gaccgttata gcctttatcg ccgttaatcc
     3841 agattaccag tttaccttct tcgattttgg cgagagccga ggcggaaaac atcatcgtcg
     3901 ttaatgcgga taatgcgagg atgcgtgcac ctgtttttat tttcataatc tatggtcctt
     3961 gttggtgaag tgctcgtgaa aacacctaaa cggactctag tttctttata cggcaacctc
     4021 tttccatcct ccttgcccct acgccccacc gttgctttgt gtgatctctg ttacagaatt
     4081 ggcagtaatg tggagatgcg cacataaaat cgccacgatt tttgcaagca acatcacgaa
     4141 attccttaca tgacctcggt ttagttcaca gaagccgcgt tctcatcctc ccgcctcctc
     4201 ccccataaaa aagctagggg gtggaggatt taagccatct cctgatgacg catagtcagc
     4261 ccatcatgaa tgttgctgtc gatgacaggt tgttacaaag ggagaagggc atggcgagcg
     4321 tacagctgca aaatgtaacg aaagcctggg gcgaggtcgt ggtatcgaaa gatatcaatc
     4381 tcgatatcca tgaaggtgaa ttcgtggtgt ttgtcggacc gtctggctgc ggtaaatcga
     4441 ctttactgcg catgattgcc gggcttgaga cgatcaccag cggcgacctg ttcatcggtg
     4501 agaaacggat gaatgacact ccgccagcag aacgtggcgt tggtatggtg tttcagtcct
     4561 acgcgctcta tccccacctg tcagtagcag aaaacatgtc atttggcctg aaactggcag
     4621 gcgcaaaaaa agaggtgatt aaccaacgcg tcaaccaggt ggcggaagtg ctacaactgg
     4681 cgcatttgct ggatcgcaaa ccgaaagcgc tctccggtgg tcagcgtcag cgtgtggcga
     4741 ttggccgtac gctggtggcc gagccaagcg tatttttgct cgatgaaccg ctctccaacc
     4801 tcgatgctgc actgcgtgtg caaatgcgta tcgaaatctc ccgtctgcat aaacgcctgg
     4861 gccgcacaat gatttacgtc acccacgatc aggtcgaagc gatgacgctg gccgacaaaa
     4921 tcgtggtgct ggacgccggt cgcgtggcgc aggttgggaa accgctggag ctgtaccact
     4981 atccggcaga ccgttttgtc gccggattta tcggttcgcc aaagatgaac ttcctgccgg
     5041 taaaagtgac cgccaccgca atcgatcaag tgcaggtgga gctgccgatg ccaaatcgtc
     5101 agcaagtctg gctgccagtt gaaagccgtg atgtccaggt tggagccaat atgtcgctgg
     5161 gtattcgccc ggaacatcta ctgccgagtg atatcgctga cgtcatcctt gagggtgaag
     5221 ttcaggtcgt cgagcaactc ggcaacgaaa cccaaatcca tatccagatc ccttccattc
     5281 gtcaaaacct ggtgtaccgc cagaacgacg tggtgttggt agaagaaggt gccacattcg
     5341 ctatcggcct gccgccagag cgttgccatc tgttccgtga ggatggcact gcatgtcgtc
     5401 gactgcataa ggagccgggc gtttaagcac cccacaaaac acacaaagcc tgtcacaggt
     5461 gatgtgaaaa aagaaaagca atgactcagg agatagaatg atgattactc tgcgcaaact
     5521 tcctctggcg gttgccgtcg cagcgggcgt aatgtctgct caggcaatgg ctgttgattt
     5581 ccacggctat gcacgttccg gtattggctg gacaggtagc ggcggtgaac aacagtgttt
     5641 ccagactacc ggtgctcaaa gtaaataccg tcttggcaac gaatgtgaaa cttatgctga
     5701 attaaaattg ggtcaggaag tgtggaaaga gggcgataag agcttctatt tcgacactaa
     5761 cgtggcctat tccgtcgcgc aacagaatga ctgggaagct accgacccgg ccttccgtga
     5821 agcaaacgtg cagggtaaaa acctgatcga atggctgcca ggctccacca tctgggcagg
     5881 taagcgcttc taccaacgtc atgacgttca tatgatcgac ttctactact gggatatttc
     5941 tggtcctggt gccggtctgg aaaacatcga tgttggcttc ggtaaactct ctctggcagc
     6001 aacccgctcc tctgaagcag gtggttcttc ctctttcgcc agcaacaata tttatgacta
     6061 taccaacgaa accgcgaacg acgttttcga tgtgcgttta gcgcagatgg aagtcaaccc
     6121 gggcggcaca ttagaactgg gtgtcgacta cggtcgtgcc aacctgcgtg ataactatcg
     6181 tctggttgat ggcgcatcga aagacggctg gttattcact gctgaacata ctcagagtgt
     6241 cctgaagggc tttaacaagt ttgttgttca gtacgctact gactcgatga cctcgcaggg
     6301 taaaggtctg tcgcagggtt ctggcgttgc atttgataac gaaaaatttg cctacaatat
     6361 caacaacaac ggtcacatgc tgcgtatcct cgaccacggt gcgatctcca tgggcgacaa
     6421 ctgggacatg atgtacgtgg gtatgtacca ggatatcaac tgggataacg acaacggcac
     6481 caagtggtgg accgtcggta ttcgcccgat gtacaagtgg acgccaatca tgagcaccgt
     6541 gatggaaatc ggctacgaca acgtcgaatc ccagcgcacc ggcgacaaga acaatcagta
     6601 caaaattacc ctcgcacaac aatggcaggc tggcgacagc atctggtcac gcccggctat
     6661 tcgtgtcttc gcaacctacg ccaagtggga tgagaaatgg ggttacgact acaacggcga
     6721 tagcaaggtt aacccgaact acggcaaagc cgttcctgct gatttcaacg gcggcagctt
     6781 cggtcgtggc gacagcgacg agtggacctt cggtgcccag atggaaatct ggtggtaata
     6841 gcaaaacctg ggccggataa ggcgtttacg ccgcatccgg cataaaacag gttgtcatta
     6901 tctgaaaggg gcgaaagccc ctctgattat cgggtttagc gcgctattgc ctggctaccg
     6961 ctgagctcca gattttgagg tgaaaacaat gaaaatgaat aaaagtctca tcgccctctg
     7021 tttatcagca gggttactgg caagcgcgcc tggaatcagc cttgccgatg ttaactacgt
     7081 accgcaaaac accagcgacg cgccagccat tccatctgcc gcgctgcaac aactcacctg
     7141 gacaccggtc gatcaatcta aaacccagac cacccaactg gcgaccggcg gccaacaact
     7201 gaacgttccc ggcatcagtg gtccggttgc tgcgtacagc gtcccggcaa acattggcga
     7261 actgaccctg acgctgacca gcgaagtgaa caaacaaacc agcgtttttg cgccgaacgt
     7321 gctgattctt gatcagaaca tgaccccatc agccttcttc cccagcagtt atttcaccta
     7381 ccaggaacca ggcgtgatga gtgcagatcg gctggaaggc gttatgcgcc tgacaccggc
     7441 gttggggcag caaaaacttt atgttctggt ctttaccacg gaaaaagatc tccagcagac
     7501 gacccaactg ctcgacccgg ctaaagccta tgccaaaggc gttggtaact cgatcccgga
     7561 tattcccgat ccggttgctc gtcataccac cgatggctta ctgaaactga aagtgaaaac
     7621 gaactccagc tccagcgtgt tggtcgggcc tttatttggt tcttccgctc cagctccggt
     7681 tacggtaggc aacacggcgg caccagctgt ggctgcaccc gctccggtgc cggtgaagaa
     7741 aagcgagccg atgctcaacg acacggaaag ttattttaat accgcgatca aaaacgctgt
     7801 cgcgaaaggt gatgttgata aggcgttaaa actgcttgat gaagctgaac gcctgggatc
     7861 gacatctgcc cgttccacct ttatcagcag tgtaaaaggc aaggggtaat tacgccccac
     7921 agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac ctttttttat gcttcattcc
     7981 agggatatga gcgatttttt atagtaactc acttcttctt cactaagaat atccattatc
     8041 tcaatgtatt atcagagatt cttttccttt cgccggtagt gtctggacgc tcaggctact
     8101 ttttccagtt atttcatttc tgtaatgcag aggttttatg ttaggtcata tctcaaagtt
     8161 tgacggcaat aactctttga taaaacatgg tgtggtgcaa ggaaataata tagtagattt
     8221 tgatttact
//
LOCUS       DAZFKY010000191         8129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFKY010000191 DAZFKY010000000
VERSION     DAZFKY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(534..674)
                     /locus_tag="ACHKCL_003988"
     CDS             complement(534..674)
                     /locus_tag="ACHKCL_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5764861.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(976..1239)
                     /locus_tag="ACHKCL_003989"
     CDS             complement(976..1239)
                     /locus_tag="ACHKCL_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5764862.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1664..1789
                     /locus_tag="ACHKCL_003990"
                     /pseudo
     CDS             1664..1789
                     /locus_tag="ACHKCL_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1854..2114
                     /locus_tag="ACHKCL_003991"
                     /pseudo
     CDS             1854..2114
                     /locus_tag="ACHKCL_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2164..2403
                     /locus_tag="ACHKCL_003992"
                     /pseudo
     CDS             2164..2403
                     /locus_tag="ACHKCL_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2451..3068)
                     /gene="ureG"
                     /locus_tag="ACHKCL_003993"
     CDS             complement(2451..3068)
                     /gene="ureG"
                     /locus_tag="ACHKCL_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO5764863.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3080..3754)
                     /locus_tag="ACHKCL_003994"
     CDS             complement(3080..3754)
                     /locus_tag="ACHKCL_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO5764864.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3755..4219)
                     /gene="ureE"
                     /locus_tag="ACHKCL_003995"
     CDS             complement(3755..4219)
                     /gene="ureE"
                     /locus_tag="ACHKCL_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO5764865.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4229..5932)
                     /gene="ureC"
                     /locus_tag="ACHKCL_003996"
     CDS             complement(4229..5932)
                     /gene="ureC"
                     /locus_tag="ACHKCL_003996"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO5764866.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(5925..6245)
                     /locus_tag="ACHKCL_003997"
     CDS             complement(5925..6245)
                     /locus_tag="ACHKCL_003997"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO5764867.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6254..6556)
                     /locus_tag="ACHKCL_003998"
     CDS             complement(6254..6556)
                     /locus_tag="ACHKCL_003998"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO5764868.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6647..7345)
                     /locus_tag="ACHKCL_003999"
     CDS             complement(6647..7345)
                     /locus_tag="ACHKCL_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO5764869.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            7726..8001
                     /locus_tag="ACHKCL_004000"
     CDS             7726..8001
                     /locus_tag="ACHKCL_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764870.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1978 a         2054 c         2053 g         2044 t
ORIGIN      
        1 cgttttattc tttcactttt atcaaaacat caccagaaac tggaagtgac aaccgcaata
       61 atgaacataa aaaggcaaat cagaatggca gtaaatcaat aaagtaccat ttcatatgaa
      121 tcatcagaaa aacggtggac gcgacatcca gcacgaggca aaacacagca acaaaataag
      181 ccggatatcg cgccctgtac ttttctaact caactcccgg cattgtgcaa gttttaatcg
      241 caggtggatt cagagccacg cccccattga ttcagcctga agacacgtat atccttggag
      301 aaagcaggaa tctcattgct gtgaaattcc ttacagaatc ttcagcccca ttttatgggg
      361 ctttttttgg tgcccccacg ctgttaccac actttcggga tgcgaataaa aaagcccaca
      421 gtttttacac tgcggacttt tgttttttct ttgtgatttg tgtgcaaggg tgcgactttc
      481 gaccgaacct ttggcaaccg gatagcgggg actgttgtcc ccgctgcggt ttcttacggt
      541 ttacactgca agaacgccgc aatttccgac cacatgaagc ttaccctgaa ttcacacggt
      601 gatgcgtgta acacccagac ggtcagcaac atggtcaggc ataacatgcc cacaatgatc
      661 gttttttgcg gcatagcact tgctcctttt acagagaggc gctaaccttc cacttgctga
      721 aggtagatgg tcagggcctc gggttaacgt ttaagttgac tcggggcctt tccacatccg
      781 gccttcaggt aatccttcca gccgtcagcc gaaaggcacc cgcgcgtaat gtactggttt
      841 tcagagaggt ccgcaatctt ccggtgacga gatcatctgc acacgccccg gtgggacggg
      901 ctaacggtat attcaggtgc aggatctctc tgtctggtgc gtttttaaat tgtcaaacgc
      961 accctggcgg caaaatcagc cacctttttt cgcatcaaca aaacgcccaa aacgctgggt
     1021 aaagcgtgca acattacctt ctgaagcgac agttctcagt ttacctgtat aaaacggatg
     1081 cgacttagaa gaaacgtcaa ttgtaacata tgggtaagtt acaccatcca gttcaattac
     1141 ccggtcagtt ttgatcgtcg aaccgatcct gaagtactca tcaatactgg tatcgtgaaa
     1201 tactactgta cggtattccg gatggatatt cggtttcaca acagttttcc ccctttaagt
     1261 gaaaattccg cataaaggta catattcaga cggtcagatt caactgtctg ttcttataac
     1321 ctcactctct ttcagtatta caatatgtta tataattata aactggactt cccgaaaaaa
     1381 tccattaaat aagaagcgag ccgttgcgga ggcttgctct ctcctttaac ctcatgtgca
     1441 atatccgtaa cgcgctcaca taaatcatca tctgataata taacaacacc cattatttta
     1501 ttctgaccta aaaacttctt atgaaataaa cgtttacccg acgtatctga tcccgctgtt
     1561 tttacctgta tcctttaaca tacttgagaa gcccgctgaa aaaatatacc gtaaaggcgg
     1621 tttctctgtc ttccataaac ctgtcggata agaagggcat taaatgaaaa ccaaaaaagt
     1681 tatgatgatt gcactggtca gctctgcgct ggctctttca ggatgtggag ccatgggcac
     1741 agcgatcaaa aaacgaaatc tggaagtaaa aacccagatg agtgaaacct gaggtagcct
     1801 gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca
     1861 gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa
     1921 atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac
     1981 aatgggttac cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac
     2041 tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata
     2101 tattaaaaaa agcagctgta gattcaatct gtcaatgcaa cacccctttc aattatctct
     2161 ttcactgccc gcgacggtat cggttatcag tttgacctgc tggctaacgg tgaaaccgta
     2221 ctgcgtgatg catccggcga aaaacgcatc aagggtgaaa aagacggtcg tatgctcatg
     2281 aatgttggcc tgaatgcgga aattcgcgac aacatccgct tcggtctgga gtttgaaaaa
     2341 tcggcatctg gtaagtacaa cgtggataac gcggtgaacg ccaacttccg ttattccttc
     2401 tgataaaaac aaggggccgc ctgccggccc tgatacagag gatgttgtga ttacttaccc
     2461 agcatgccct gctcttcgat aaaggcgatg atgttttgca gaccttcacc acttttcagg
     2521 ttagtgaacc cccatgggcg ttctccgcgc atacgtaaag tatcacgctc cattacctcg
     2581 agggaggcgc ccacgtatgg tgcaaggtca gttttattga tcacgaggaa gtcggatttg
     2641 gtgatccccg gaccgccttt acgcgggatc ttttctcctt ctgccacatc tatgacatat
     2701 atggtaaggt ccgccagttc ggggctgaag gtcgcactca ggttgtcgcc gccgctttcc
     2761 acaaagatca gctcgagatt accaaatttc tcactcagtg cttccacggc tgccagattc
     2821 attgatgcgt cttcacgaat ggctgtatga gggcagcccc ccgtttcgac accgacaatg
     2881 cgttccgctt ccagtgctcc ggcctcagtg agaatgcgtt gatcttctct ggtgtagatg
     2941 tcgttggtga ctacggctaa ttgccaggtg tcacgcatgg atttgcagag ggcttccaga
     3001 agcgcggttt tacctgagcc gaccggaccg ccgacgccga cacgcagcgg ttgcttgatt
     3061 atgttcattt tattttctcc taagaacgga ataatcggga atactgggtt tcatgtcgcg
     3121 cggaagcgat agatgcaagt ggcgttgctg aaccaattgc gtgatccggg gttgcaaggg
     3181 cactgtccat gttcgccgca tagcgggcac acaggcgcat aattaactgc tgggcagcca
     3241 gctggccgta gggtaccagc ctgacgcccg ccatcactgc gctttcaatc cagctgtagc
     3301 caaggctcag ggccaggtct gaaacggata tttgccagcg tacgccgagc catgccatac
     3361 cggttaactg gctttgctgg catagtttgc gccattcggc cgggcagtcg ggctgccagt
     3421 cgaccagaag tctggtgaag gctgcaccgc ggttacgttc ttcatcgcgt agctcacggg
     3481 tttccctgca ggccagcaga taagcggtcc agcgcccggc actgtcagga tcgcccgctt
     3541 cacaggcccg gtatagccgg gcaagcagtg gcaggtcgac ggcaaaaaaa ctctgttcca
     3601 tctgcaactg ctgccagtgt tcaaaggcgg cgctatcttc tacccagcct gcttccactg
     3661 cccactccag tccctgggac cagctgtagc ctccaacagg gaggctgtta ctggcaagct
     3721 gcatcagccg cagacgtttt tccggagtcg gcattcagtg gctgtgctcc tgatcgtgat
     3781 tatgggcgtg cgacttgctg gcgtaagcgc cggcttccgg ctcaaacgga agatgtgcaa
     3841 aatcaacatc cagcccaaac tggcgcagca tatcatcgag gacgtgatcg tgatggtagc
     3901 gcagctctcc gggcattatt tgcagcggaa cgtggcgatt gccgagatgc cagcaggctt
     3961 tcgcgagcat aaaaggatct gcgcagcgca ctacagaaac ggcttcatca gcggcgatca
     4021 ccttaatgaa ttcatcaccg ttttcgttac tgagaatatc accatcgcgc agcagcagac
     4081 cgcgtggcag caacagtcca gcctgacgtc catcgttaag ggtgacttta atacggcttt
     4141 ttacccgcat gtcaacaggg agggtaacgc tggcagttgt ctgagccggg gtctccaccc
     4201 gccgggtcaa ataaagcatt tgtgctcctt aaaacaggaa ataacgttgc gccattggca
     4261 gaatgtccgc cggttcactg gttatcagtt cgccgttgat acgcacttcg taggtttgtg
     4321 aatccacggt tatatcaggc aacaggctgt tgtggcgcat atcggctttt tgtactgtgc
     4381 ggcagccttt taccacagca gttgtgctgt gcaggttaag ctgttcagcg acgccatttg
     4441 ctgctgctgc ctgcgacagg aaagtcacac gacagcggtg acgggcactg cccaatgcag
     4501 cgaacattgg gcgatagtgc accggctgcg gtgtggggat agagccgttg atatcaccca
     4561 tcggcgccat ggctatcatt ccgcctttga cgatagtcgc cggttttacg ccaaagaacg
     4621 ccggggacca gagcaccaga tccgccagtt ttcccacttc aatcgagccg acttcatgag
     4681 caataccgtg ggttaatgcc ggattaatgg tgtatttagc gatatagcgc ttcacgcgga
     4741 cgttgtcgtt atcaccactt tcttccggta acgggccgcg ctgaactttc atccggtgtg
     4801 ccacctgcca ggttcgtaac actacttctc cgacgcgtcc catggcctgc gaatcggacg
     4861 aggtgaggga gaacgcgcca aggtcgtgca ggacgtcttc cgcggcaatg gtttcctggc
     4921 gaatgcgcga ttcggcaaag gctacgtcct cggcgatatc cgggtcaaga tgatggcaaa
     4981 ccatcagcat gtccagatgc tcatcaatag tgttgacggt atagggtagc gtcggattgg
     5041 ttgaggaagg cagaatattg gggtgcgcgc aggcggtgat aatatccgga gcatgaccac
     5101 cacctgcacc ttctgtatgg aaggtatgga tagtgcgccc gccgatggca gtcagagtat
     5161 cctcaacgaa tcctgattcg ttaagcgtat cactgtgtag cgcaacctga acgtccattt
     5221 cgtcggctac agtcagtgcg cagttgatta ccgcaggtgt agctccccag tcttcgtgaa
     5281 ttttgaggcc gataaccccg gccgcgacct gctcacgaag cgcatccgga ttggagcaat
     5341 tgcctttacc cagcaacccg atattgaccg gcaggctgtc tgcagcctgc agcatttgat
     5401 aaatgtacca tggtcctggg gtacaggttg tggcgttaga acccgctgtc gggccagtac
     5461 cgccaccgat catggtggta atgccggatg tcagcgcttc ttcagcctgc tgtgggcaga
     5521 tccagtgaat atgcgtatcg acgccacctg cggtaacgat cctgccttct gcggcaataa
     5581 tttccgtgga tacgccgatt gggatagtga cgttgggttg tatatcagga ttaccggctt
     5641 tgccgatagc aaatatcctt ccatctttga cgccgatatc ggctttaacg atcccccagt
     5701 aatcgatgat cagggcattg gtcagcacca gatcagcgca gccggcggag agcatttgcc
     5761 cctgtcccat accgtcgcgg attactttac cgccgccgaa tttgacctct tcgccgtagg
     5821 tagttaaatc atcttcgacc tcgatccaca gctcagtgtc tgccagacga attttatcac
     5881 cggtggtagg gccgaacatg tcagcatagg cctggcgtga aatattactc atcatttgcc
     5941 tccagctcac ccattatatc tccgcggaaa ccgtgcacga tacgggcacc gctcagcgcc
     6001 accagttcaa cctcacgctt ttggcctggt tcgaagcgca ctgcagttcc ggcggcgata
     6061 ttgagacgat agcctcttgc cttctggcga tcgaacttca gtgccgggtt aacctccgcg
     6121 aagtgataat gggaaccgac ctgaattggt cggtctccgt gattttcaac gataatactg
     6181 caggtagccc ggccgatgtt aagctctata tagccaggtt taaccttata ttctccaggg
     6241 atcatctgtt acctcagatt atcggattat ggacggtaac cagcttggag ccgtccggaa
     6301 aggtggcttc gacctggata tcggggatca tttctggtat gccttccatg acctgctcgc
     6361 gactcaggac atgccgtcct tcttccatca gcgcagcgac gcttttgccg tcgcgagcgc
     6421 cctccattat aaaagcgcta atcagggcca cggattcggg atagttaagt ttcaggccgc
     6481 gggccagacg acgctctgcc agcagtgcag cggtaaacag taatagtttg tctttttctc
     6541 ttggagtcag ttccatagcg ttctcttaag tcagccagat ccggggaagt accggtgatt
     6601 tgtcagtcag ttgcggccgc ataaattgcc agatatcacg cattacctac tggcaacgca
     6661 gattatcatc acttaaaaag cgaactgtca gtagaccatc ggtgagcgtg gctccggcgt
     6721 agttttccag tggtgtgagt ttttcgcgta tgtcttccag ttgcgtttca ttccccaggt
     6781 agaacagcat cgttcccacc catggtttcc gggcaacgca ggtaagttct ccattggcca
     6841 cgtgcagacg ctcaatcaac agcggagaac cgtcgaccca tatctccaga cggttagcga
     6901 gtgttccgtg gctgaaggtt tctccgatga ccggacgtcc aaggcacaga agatcccagg
     6961 ccagcagtgt actggaggac gacagatgaa acacggtatg caacgtggcg tgagcgccgg
     7021 gaaaaaaaat cgaatcctgt ggcaaccact ccagcgttgc gttattgcac agcgtcaggt
     7081 tttgctgaag gtgcgcctgc gcgccgctgc tgcggtaaaa tttactggca ccaggcatgg
     7141 tgatcagcgt atggcaatca gagtcaatta ttgcgttgat gatgagttcg tcaccgccga
     7201 cgataccacc gggtggatga agcagacaga ggtggcaggt ttcttcttcg ggataaaaag
     7261 gacgctgaac agtgagcaga ccgacatgat gacgggtggt gagcgtggtt ttcccgtgca
     7321 gacgctgaaa acggagatcc agcatggcct gccagccttt tttattttgt ggtaacgcgg
     7381 tactgagcaa tcttacgccc ctgataaata aagataaaac gacagtaaag agccggattt
     7441 ccttgttata taaaagatga tgcctttcca gtgagttttc gtctgaataa tacgaatgtt
     7501 tgttttcgtc atgaagcgtt ccggctcccg gggctataca tcgccagaaa agcaatgcat
     7561 attgtcggga ttatccagag aatcaggcag aaagatccga caatgggctg tgattatgcc
     7621 atctggctca gaatgatatc aaggtgttat ggggataact tgctgataca atagcagtat
     7681 gacagattca ttaatgcgga aactgctcac tgaaacagag tgttcatgac tgagcactgg
     7741 tcgggtcatg cagatgataa tttccccttt tcttggggaa gcgacagtat tttcatgcat
     7801 gcctgtcttc agtcatacgg ggagagcatc tgtgatacag tccatacatt tgttgcggac
     7861 gtcgctcctg agtcactgac tggtctgcaa acagtttatc tgtacacggc agggcttctg
     7921 tgtgatatcg tttttcatat cgaggggggg cctccagaga tgaatggaat aataatattt
     7981 cctgatgata taatacctta gagaatttct gtgtatgcta ttgtgaacat attttttatt
     8041 aaaaagatgt tagtggtgtt gtaaaataaa agaatcagta actataaacg cctttattga
     8101 cataaagttt tgatgatgaa gttgaagtt
//
LOCUS       DAZFKY010000192         8049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFKY010000192 DAZFKY010000000
VERSION     DAZFKY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(68..259)
                     /locus_tag="ACHKCL_004001"
     CDS             complement(68..259)
                     /locus_tag="ACHKCL_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5764871.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKCL_004002"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHKCL_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5764872.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ACHKCL_004003"
     CDS             1018..1203
                     /locus_tag="ACHKCL_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764873.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ACHKCL_004004"
     CDS             complement(1390..1779)
                     /locus_tag="ACHKCL_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764874.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHKCL_004005"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHKCL_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO5764875.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /gene="ydfA"
                     /locus_tag="ACHKCL_004006"
     CDS             complement(1921..2076)
                     /gene="ydfA"
                     /locus_tag="ACHKCL_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5764876.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ACHKCL_004007"
     CDS             complement(2354..2641)
                     /locus_tag="ACHKCL_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5764877.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ACHKCL_004008"
     CDS             complement(2641..2832)
                     /locus_tag="ACHKCL_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5764878.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ACHKCL_004009"
     CDS             complement(2860..3261)
                     /locus_tag="ACHKCL_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5764879.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ACHKCL_004010"
     CDS             3370..3642
                     /locus_tag="ACHKCL_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5764880.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ACHKCL_004011"
     CDS             3626..4051
                     /locus_tag="ACHKCL_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5764881.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ACHKCL_004012"
     CDS             complement(4258..4713)
                     /locus_tag="ACHKCL_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764882.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5883
                     /locus_tag="ACHKCL_004013"
     CDS             4792..5883
                     /locus_tag="ACHKCL_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5764883.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5890..6636
                     /locus_tag="ACHKCL_004014"
     CDS             5890..6636
                     /locus_tag="ACHKCL_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5764884.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6658..7428
                     /locus_tag="ACHKCL_004015"
     CDS             6658..7428
                     /locus_tag="ACHKCL_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5764885.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            7444..7857
                     /locus_tag="ACHKCL_004016"
     CDS             7444..7857
                     /locus_tag="ACHKCL_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5764886.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         2151 a         1726 c         2098 g         2074 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gttgattgca tacagttttt tattgtaggc tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggagcagg agaaagaaca ggaacaggac aaaaacacta tggttcatgg caaaaaaaac
     5341 accacgaacc aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca
     5401 ccggaagccg attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga gcaatcacac
     5461 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
     5521 gaaagcaccg ggcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     5581 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
     5641 gccagattgc gcgagggggt gccaccagag accatgctgg atggtgtgag gcgttacgcg
     5701 agatacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgagc gacgacgttt
     5761 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
     5821 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
     5881 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
     5941 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgct
     6001 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
     6061 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     6121 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     6181 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     6241 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     6301 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     6361 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     6421 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     6481 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gtacacttct tggtgagagg
     6541 attatggacc gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg
     6601 cgtccaaatg tcagcaatat gagggttgtg aagtaatttt gtccggagga aattttaatg
     6661 gaaaccgtat ctgacgcact gaaagcactg aaaaaagcct cttcacatgt ggtggcagct
     6721 cgccttggaa tcagtcgtga agaggctgtc aacgagctgt gggaactcaa aataaatggc
     6781 gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc
     6841 gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa
     6901 gttaccgcag acatgatgat tgagtttatc ggtcaggatg gggctaaaac gtgtgaggaa
     6961 ctggcgggta agttcggtgt cagtactcgc aaggttgctt ccacgctggc ggtggtaacc
     7021 gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc
     7081 gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt
     7141 cctcagccag caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa
     7201 acagaaactg tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat
     7261 gagctgattt ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat
     7321 gttcagaagt gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt
     7381 gttcgacaga ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg
     7441 cttatggcaa aagtatttac acaggaagag cgggaaaaaa ttaaagggca ggttgttgag
     7501 ctagtacgcc ggagtgggcg cgagacgtta cggcaactgg aagccaagac aggtgcgaca
     7561 agatatctga tgagcgttct tgccagagag ctggttgcca gtggcgatgt atacaactct
     7621 ggttacgggt tattcccatc tgaacaggct cgtaaggact ggcaaaacgc ccgcaaaaaa
     7681 ctatcgaggg caaaactgaa gaaaccggtt gtggttgatc ctgaccttat ctggtcatta
     7741 ccagacggag aaatacgccg ctatgacagg caattgaaca taatttgtag tgagtgccgt
     7801 aacagtgaag tgatgcagcg agttttgata ttttacacag gagtaatgat ggaatagtga
     7861 aaataacatg aaacctttgg attctggtgt ttcagtggat gggaaaagag cagatacata
     7921 gaaaatgaat agcaataatt cacagtctgg atgttgtttg tatgcttata aaatgatcct
     7981 agtatgcatt gccggagttg aatcagctcc aatgaagaaa ctgagaggag tatttagaag
     8041 atagctaaa
//
LOCUS       DAZFKY010000193         8032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFKY010000193 DAZFKY010000000
VERSION     DAZFKY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHKCL_004017"
     CDS             complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHKCL_004017"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5764887.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHKCL_004018"
     CDS             complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHKCL_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5764888.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2889..3079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3193..4293
                     /gene="trmA"
                     /locus_tag="ACHKCL_004019"
     CDS             3193..4293
                     /gene="trmA"
                     /locus_tag="ACHKCL_004019"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5764889.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHKCL_004020"
     CDS             complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHKCL_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5764890.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHKCL_004021"
     CDS             complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHKCL_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5764891.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5446..6762)
                     /locus_tag="ACHKCL_004022"
     CDS             complement(5446..6762)
                     /locus_tag="ACHKCL_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO5764892.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6805..7971)
                     /locus_tag="ACHKCL_004023"
     CDS             complement(6805..7971)
                     /locus_tag="ACHKCL_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO5764893.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2101 a         2179 c         1904 g         1848 t
ORIGIN      
        1 gctacagagt caagcattta tttttgcttt ctctgtcgga attctcagga gaaccccgct
       61 gacccggcgg cgtgtttgcc gttgttccgt gtcagtggtg gcgcattata gggagttatt
      121 ccggcctgac aagaggaaat ttaaaataat tttccgaccg cgcaacattc aaccaaatca
      181 gcctaaaact gccagttttt cgagcgtttc gaagccgtaa cgctgtaaaa cgggcaataa
      241 ttgttcagct tctggcgtca ttgccataca aaaggcaata ttcgcatcgg cagattttgc
      301 atccggggct tcatgttcta acaaccaggc cgttcggcga gcaatcgctg cgccagaatc
      361 caccagccgg gttccctctg gcagcacttg taacagttct tcttgtagta gagggaaatg
      421 ggtgcaaccc aatacaacgg tatctggcgg ctctttcatt cttaaccacg ggcgtaggat
      481 acgttttagt gcatccagag aaacatcttc gccatgcagc ttcgcttcag ccaactcaac
      541 catctctgcc gagcccagca tttctatctg gcattcatta gcgaaacgcg cgatcagctc
      601 atgagtataa gaacgtttaa ctgttccgcg ggttgccagt aatccgacaa tgccatttgc
      661 cgtcagacgt gcagcaggtt taatcgccgg cacgacacca acaaccggga agtcgaactt
      721 ttcgcgtaat gcaggaagtg aaacggtact ggcagtgttg caagcgacca cagccagcgc
      781 aaggggataa cgttcttgca ccgcggtgac aattgccacc actcgctcaa caataaacgc
      841 ttcgcttttt tcgccatacg ggaaagcgac gttatcgaaa gcataaatgt aatggagatc
      901 cggtaagaga tgccggatct cgtcatagac cgacaaccca ccgacgccgg agtcaaacac
      961 cagcacggtg ggacgtggtt cagaaggtgt agctgccaga caaggtgtat tcccgtcctg
     1021 cagtttggta gccatagact gtctcataat ctttgtcgaa caggttggct attttaccac
     1081 gaactgtcag gtgagaggtg accggatacg caaccgcaag atcccacaag ctcacaccgc
     1141 ccattttaac ggtttgataa ggataagatg agtaatcctt atcatagcga gtgcctaaat
     1201 actgataagt aataccccag tcgaagtcat acaactgcca gtcgagctgg tatttcacct
     1261 gctgtttagc acggcgtaac aacggcgtgt cggtaattgc attgcgcgca tcgacataat
     1321 cataactcac agtatgcgtc agtggtccgg tatcaaaatt ggcggtcgcc tcgacgccct
     1381 taatccgcgc tttcccttcg ttgtaatatt tcagggtgtg atcatcataa tcgatcaagt
     1441 cactgacatc gttacgatat ccggaaatac gccagttcac cccagcggtt aagccttcaa
     1501 acgcgccttc ccactgtttg cttttctccg ggtccagatt cggatttccg tagaagccat
     1561 acagttgccc cagatttggt gccttataag atgtcccgta ggaagcaatg aagcgataac
     1621 cttcgatgaa ttcccaaccg gcgctggttt gccaggttcc atgacgacca aactgtgagt
     1681 tatcgtcgct gcgtgctgcg ccttcaaagg taaaatcgcc gacttgttgc agcccggtca
     1741 gatagatgcc ggtattacgt tgatcatatc catccttaac ataagctgtg cccggtgccg
     1801 tgctctgctt ctgccagtca acacccgcac caacattacc gtggccaatg atgatgttgt
     1861 ttgcccactg gacggtgtat tgcttcatct catcgagcgt cgccgacgaa tcataacgac
     1921 cataatgggg atcgtagttg taatctttgc tatggctata gctggtaatg agttgtgatt
     1981 taatcagttc gccgttatat cgcagcccgg cgtcccaact ttgactatag agtttacggg
     2041 tatcgaccaa tggtgaaccc ggagaataat acgcgtcata attggtacgg ttatcatagc
     2101 catagccgcg cacaaagccg ctccaggcat cagtaaagtt atgctccagc gcgccataaa
     2161 gcgttttact taaaaaacca tcgttatctg gctgcgcttg cgttccggta ttaccatagg
     2221 caacaacatc ataaccatga gtatgggcat aatcgcccaa caacgttact cgtgtcttat
     2281 cccccagttg ttgctgtgta gagacatcat agttttgata actattgctt ccccaccctg
     2341 ctgaaatttc cgttccgggt tcatcgcgcg tcgtgatgat attcaccacc ccacctattg
     2401 catcggaacc ataaacggcg gagcgtggcc cacggatata ttcaacacgc tggacaagcg
     2461 caatagggaa ctggctaagg tcggcagaac cactcccccc cgccagattc aggcgtacgc
     2521 catcaattaa caccaacaca tgactggcat ttgtaccgcg aataaaaata gatgagagct
     2581 gacctgaacc gccgttttgg gtgatatcga cgcccggaag acggcgcagc acatcattaa
     2641 ccgaggtcga ctgccagcgg tcgatatcct gacgcgtcac aacggtggtt ggtgcaagca
     2701 cagtgctgcg cggctgttca aaacggttag cagtaacgac gagagtatcc gggctggtat
     2761 cctgtgccca agcggaaaag gctgtgacag aacacgccgt cagcagcgaa gcttttttaa
     2821 tcattgtaaa gcatccacaa tagaagaagg atgccgcagg tttcatcaat attacgcgat
     2881 gatgagaacc agatgcgacg ttggccggca ggtcttcggg cttggagggg tatctaagat
     2941 actaagagat gatgacttcc caccgaatgg cagtgtccgc ataacgcaat catcgcacct
     3001 ttccttaccg ctgcgcgtca gctccagatt cgcactggat tccctattaa ctcacaggac
     3061 cggcaagtgg atgctacagg ttgtaacaag ttactgtcca gacgtagctc acaaatagga
     3121 attcatcaag atctggacat ctgatgagca atccctacaa tcgccgcgta ctttaatttt
     3181 tcaggataca tcatgacccc cgaacacctt ccaacagaac agtatgaagc gcagttagcc
     3241 gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt ctgacctggt tccggaagtg
     3301 tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt tccgcatctg gcacgatggc
     3361 gatgacctgt accacatcat tttcgatcaa caaaccaaaa gccgtatccg ggtggatagc
     3421 ttccccgccg ccagtgaact catcaaccag ttgatgacgg cgatgattgc gggtgtgcgt
     3481 aataatcccg ttctgcgcca caagttgttc cagattgatt acctcaccac gctgagtaat
     3541 caggcggtgg tttccctgct gtaccataaa aagctggatg atgagtggcg tcaggaagcg
     3601 gaagccctgc gcgatgcact gcgcgcgcag aatctgaatg tgcatctgat tggtcgggca
     3661 acgaaaacca aaatcgcgct ggatcaagat tacatcgacg agcgtctgcc ggtcgcaggg
     3721 aaagagatga tctatcgtca ggtagaaaac agctttaccc agccgaacgc ggcgatgaat
     3781 attcagatgc tggaatgggc gctggacgta accaaaggct caaaaggcga tttactggag
     3841 ctgtactgcg gcaacggtaa cttctcatta gcgctggcgc gcaattttga tcgggtatta
     3901 gccaccgaaa tcgccaagcc atcagtcgct gccgcgcaat acaacattgc cgctaaccat
     3961 attgataacg tacaaattat tcgtatggcg gcagaagaat ttactcaggc gatgaatggc
     4021 gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga gttatcagtg cgaaaccatt
     4081 tttgtcgacc cacctcgcag cggtctggac agtgaaaccg agaaaatggt gcaggcgtat
     4141 ccacgtattc tgtatatctc ctgcaatccg gaaacgttat gcaaaaatct ggaaacatta
     4201 agccagacgc acaaggtcga acgtctggct ctgtttgatc agttccccta cacgcaccat
     4261 atggagtgcg gcgtattact gaccgcgaag taaaacccat gccggatgcg ccagcatccg
     4321 gcatgatacc gattactcag caacttcctg cttacggtta cgcatcttcg cgccaatcca
     4381 gaacaccata atgacggaga gtactgccgg gaagaagtta gagccgatat ccggatattc
     4441 cgcacgaact accgtgctgt acagcagtac gccgagaata aaacaggcag ctgccagacc
     4501 cggcaagcct accggcatgg tgcgattaag ataacgttga tgcaggcagt aaaccgtcag
     4561 caccagggaa ataatcggga atacagaaaa tggcacaatg gagctaaaca gcgctgcgaa
     4621 agtaccatta atcgataagc cagcaaccaa cgccagcagc agcgtacctc tatcttgatt
     4681 tgcttgtttc attactcgtc cttcacattt cccggaataa ttgcggtttt ctcttgttca
     4741 cggcgatacc agtaataagc ccctttcgaa atcattcgca gttgcagtac cagtcgctct
     4801 tctaattgcc gacgttgttc aacgccgacg tccaacgcct cggcacccgc actgaagaca
     4861 attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac gcggcatatg gttttcgagt
     4921 tccagatagt ccgcaagttc cgcaatgaag tgctgaattt cacgcgcaac ggcggcacga
     4981 aacgcagcgg aggtgccgga gcgttcccgc aataataacc ggaaggcgtt aggattatta
     5041 ccgatgaact ccataaatgt ggagaccgag gtgcggatca cactcccgcc tttggcgata
     5101 cgctgacgcg cctggcgcat gagttggcgt agcattaaac cgctctcatc aaccatggtc
     5161 agacccagtt cgtctacgtc tcggaaatgc cgataaaaag aggtgggagc aatgcccgct
     5221 tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc gttcagcact taattggcta
     5281 aatgcggctt ccaccagcga acggcgggtt ttttcttttt gttgcgctct tacgcccatc
     5341 acgatgtctg aatccttgcc aaaagtactg ctggcactat accagagaat gaacatactg
     5401 gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa catatttact gcaattcttt
     5461 ggccggttct tttacgtaca acaacgccaa catcgacagc aacgtgcctc cacccagata
     5521 ccacgccgga gccgtcaaac tatccagcaa cacgatggat tgcgtagcga aatattgagc
     5581 aaaaccgcca aagattgcca caccaatgct ataaaccagc gcaaaaccca atgcccgaat
     5641 ccgtttcggc aacagttcag agatcaacaa catcactggc acaccgccaa gcgtggtaaa
     5701 gctgaccatc acgaaaacca ccgtcagcaa catgcctggg cttgggtaat tcaccaacag
     5761 ccagaatgac ggccagctac agatcagcac catcacacgg gagattaaaa tcagcttttt
     5821 acgcccgacc gaatcgcaca acatccccac cagcagcgca cccacaaacg taataacgcc
     5881 cgccagtaac atcgctgcat gagaatagtt ttggttcatt cctaaatact tcgccgccca
     5941 tgtaccgtaa taaaagaggg agatataggt tgcgactgta ctaccaatag cgagcaagat
     6001 accgttagcg atagtcgctt tatgttgcat aagtaaggag aaggcgcttt cgctggctgc
     6061 ggccttctta ttatgtgctg gctcaggaac atcattttcc agactcaaac gcaaccaaca
     6121 gccgacaggc gctaataaca cgccaataaa aaacggcaca cgccagcccc attcagccat
     6181 gacggtttct gaaccggtcg caaagggcag ccatgcactt aaacctaaag cgacaacacc
     6241 tccaaaagtt gttgccaggc cttgagtcgc cagtgaccaa ctgctgtaaa aaccgcgacg
     6301 atttgccgga gctgattcaa ccaacaaaga cattgaggca ccgacttcgc ctcctgccgc
     6361 aacgccctga attagacgcg ctagcaccag cgtcgcggtt ccccaatagc ccgccgtttc
     6421 atacgtcggt gcgatgccga tcatcagtgt accaagactc atcagcgcaa tggtgaacac
     6481 catcgcgggt ttgcgtccga aacgatcggc ccaggccccc acaataatgc cgccgagcgg
     6541 gcgcataaaa aagctcacgc cgaaagtggc aaacgccagc agtagcgcga cgtttttatc
     6601 tgcactgggc ggaaaaaata gcgtgctgat atagaccaca aaaaagttgt aaacgccaaa
     6661 atcaaaaaat tcgagcgcat tgccaaacgc cgaggcaaaa atcacccgcc tgccgctggg
     6721 ctttttcttc acactcacgg tttcatttac gatcgccgtc attccatgtt ccttgccaaa
     6781 aagtgttgtt gtgtgttgtt ttatttattg cagccaggct tctaccagtg cgccccagta
     6841 gctgcttgcc gggaccagac ttgcatcgtt gaagtcgtaa cccgggttat gcaccatgca
     6901 ggaaccttcc ccgacatcgc cattaccgaa taacaggaaa ccgcctttgg gttgggcttc
     6961 cagcatgaaa gagaaatctt cactgcccat caggggcttg atgccaaact cggcccgatc
     7021 ttcaccaaac gttttacacg ccacctgcca ggcgaagcgc gccatctcct cgtcatttac
     7081 taatactggt gtaccgttaa catgcgtaac ctcagccgta gcgccaaagc tggcagcctg
     7141 ggcttgtgcc agagcaggaa tacgggtcag aagaagttgt cgggtatcgc ggctaagaga
     7201 gcggacgctg attttcatct cagcactgtc aggaatgacg ttagccgcct cacccgcaac
     7261 aatgctgccg acggtaatta ccgccgcttc cagcggatcg acattgcgtg acacaatgct
     7321 ttgtaatgcg gtggtgatat gtgcggcaac tagcaccgga tcgatggctt tgtgcgggat
     7381 cgcaccgtgt ccgccacaac cacgaactgt aatatggaac tgatccattg acgccatcag
     7441 tgcccctggc tggaaataaa acttacccac cggcagaccg ggcatgttat gcatgccgaa
     7501 aatgacatcg caggggaaat gatcaaaaag cccctcttta accatgatct caccgccatt
     7561 aatcatctct tccgcaggct ggaagataag acgtaacgtg ccgttaaaac ggcgggtttc
     7621 ggcaaaataa cgcgcggccc ccagcagtat ggtggtatgc ccgtcatgcc cgcaggcgtg
     7681 cattaaacca ggatgtttac tggcccaggg cttgccacta ttttcataaa tcggcagcgc
     7741 atccatatcc gcccgcaggc caatactttt ctctccgtcg cccactttta acgtggccac
     7801 tatccccgtc gtcgccagtc cttgctctac ttcatagccc cactgacgca gcttttccgc
     7861 aaccaaagca ctggttttaa attcttgtaa tcccagctcc gggttttcgt ggatctgatg
     7921 gcggatctca cgcagttctt cctcaaaccc tcgaatatag tgttcgatca tcggctattc
     7981 ctttattcac ttataaacag gctaattaac ggattttatt gatggtgtaa at
//
LOCUS       DAZFKY010000194         8028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFKY010000194 DAZFKY010000000
VERSION     DAZFKY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(202..1395)
                     /gene="tyrB"
                     /locus_tag="ACHKCL_004024"
     CDS             complement(202..1395)
                     /gene="tyrB"
                     /locus_tag="ACHKCL_004024"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5764894.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1648..2727)
                     /gene="alr"
                     /locus_tag="ACHKCL_004025"
     CDS             complement(1648..2727)
                     /gene="alr"
                     /locus_tag="ACHKCL_004025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5764895.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2780..4195)
                     /gene="dnaB"
                     /locus_tag="ACHKCL_004026"
     CDS             complement(2780..4195)
                     /gene="dnaB"
                     /locus_tag="ACHKCL_004026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5764896.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4278..5261
                     /locus_tag="ACHKCL_004027"
     CDS             4278..5261
                     /locus_tag="ACHKCL_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5764897.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5427..5669)
                     /gene="pspG"
                     /locus_tag="ACHKCL_004028"
     CDS             complement(5427..5669)
                     /gene="pspG"
                     /locus_tag="ACHKCL_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5764898.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5803..6840)
                     /gene="dusA"
                     /locus_tag="ACHKCL_004029"
     CDS             complement(5803..6840)
                     /gene="dusA"
                     /locus_tag="ACHKCL_004029"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5764899.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7202..>8028)
                     /locus_tag="ACHKCL_004030"
     CDS             complement(7202..>8028)
                     /locus_tag="ACHKCL_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO5764900.1"
                     /translation="RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNE
                     YIGDCTDEELNDREFIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLP
                     STVQLFYEESKDNEPLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSV
                     YSDFAGRVNINEYNDNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHL
                     VNEGTLAPDILSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
BASE COUNT         2102 a         2078 c         1879 g         1969 t
ORIGIN      
        1 cagaaattat tactccacac gaaaaaaaca gggaaaggaa ttccagataa ttgggataaa
       61 cttaacaata aaagaactaa caacgtttat tatctctcac gtagaacgat ggtatcaaaa
      121 aaatcatcta taaagaaaga gtgaaagcga cgacagttta atttcactgc aggctgggta
      181 attccagcct gctttcctgc attacatcac cgcagcaaac gcctttgcca cacgttgcac
      241 atttgccgta tttaacccgg cgacacacat gcgaccgctg gcgatgagat agacaccaaa
      301 ttcttcacgt agtcggtcaa cctgagcggc acttaaaccg gtataactga acatgccgcg
      361 ctgattaagc agataatcga aattgcgttc tggcatctct gtgcttaata ccttcaccag
      421 ttcctgacgc attgccagaa tgcgagtacg catctcttct acttccgcca gccagctggc
      481 tttcaatgcc tcgtcattca gcactgcagc caccacctgc gcaccaaaat tcggcgggct
      541 ggagtagttg cggcgaactg ttgctttcaa ttgccccagt acgcggcctg cagcttcggc
      601 atcttcacac agaacagaaa gtccgccgac gcgctcgccg taaagggaga aaattttcga
      661 gaacgaattg ctcaccagag cgggtaatcc agcgctggca atggcgcgaa tggcgtaggc
      721 atcctcttcc ataccggcac caaatccttg ataggcaata tcaaggaatg ggataagctc
      781 gcgggctttg agaatttcaa tcaccgcatc ccactggtca ttagtgagat cggcacccgt
      841 tgggttgtgg caacatggat gcagcaacac aatactgcgg gcaggtaatg ttttcagcat
      901 cgccaacagg tcattaaagc gcacgccgtt agtcgcttcg tcataccagg ggtaagtgct
      961 tacttcgaat ccagccccgg cgaatattgc tacgtggttt tcccaggtag gatcgctgac
     1021 ccagacgcct gattccggga agtagcgttt caggaaatcc gcgcccactt tcaatgcccc
     1081 tgagccgcca agggtttgaa tggttgctac gcgctgttgt tgcagtaccg gatggtcggc
     1141 accaaacagc agcggcgcaa tggcatggcg atagctgtta agcccttcca tcggtaaata
     1201 aagcgaagcg ccatgaggca ccgcattcag gcgcgcttcc gcctccgcca cggctttcaa
     1261 ttgtggaata attccgtctt cgttgtagta cagaccgata cttaaattca ctttgtcgct
     1321 gcgagggtct tctttaaaac gctccataag cgtaagaatc gggtcgccag cgtaggcgtc
     1381 aactttttga aacacgcgat ggttctccag gtttacgggc aggtggttaa aacacaataa
     1441 accggaagaa ggcgaagatc gagtggatgt tcaggggcga acggcaatta gcaacagagt
     1501 gagactcatg acaaacgtac atccgccaga ggcacgacct ttataagagc atgaaagaat
     1561 atcaacttat tgaattggta ggattttatt ggccggataa ggcattcacg ccgcatccgg
     1621 cacagacaat caaatattac agaacgatta atccacgtat ttcatcgcga cccttgaagt
     1681 caggcgcgta ataagttcgt aagcgcttac tttcgtcatt tcagcgatac gttctacggg
     1741 caaaccttca ccccacaaaa tgaccgggtc cccggcttta tcctgtgcct gtggacctaa
     1801 gtctacgcag atcatatcca tcgccacgcg cccgacaatc ggcacttcgc gaccgttcac
     1861 cagcactggc gtaccggacg gcgcggcgcg cggataacca tcgccatagc ccatcgcaac
     1921 tacaccaaga cgagtatcac gttcgcttat ccaggttcca ccataaccga caggctctcc
     1981 gactttatgc tcacgcacgg caatcaggct ggaggttagt gacatcactg gctgacagcc
     2041 aaaatcggca ccggtggagc gatcttccag cggcgagacg ccataaagaa tgatgcccgg
     2101 gcgcacccag tcaaaatgcg actgtggcca cagcagaatg ccacccgacg cggcaatgga
     2161 acgttgacct ggtttgcctt cgcaaaaggt attaaagata gcgagttgtt tctcggttgc
     2221 gccacatttt ggttcatccg cgcgcgcaaa atggctgacg atattcaccg gctgccgaac
     2281 gtttttgcac tgggtcaggc gatgataaaa cgcctcagcc tgttccggcc ttacgcccag
     2341 acggtgcata ccggtatcga gtttcatcca gacggtaacc ggctcgtcca ggctagcctc
     2401 ttccagcgca gccagctgtt cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat
     2461 cgtcggcaga tctctggcat caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc
     2521 ccccgcacgc agtcgcagag cttcttcgag acgggctacg ccaaaggcgt cagcatcggg
     2581 gagcgttcgc gcggtctcaa gaagaccgtg accataagcg ttcgctttca ccaccgcaac
     2641 cattttactg gcaggtgcca gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg
     2701 gttaatcaca acagttgccg cttgcatttg tgttccttga taagtgtttg ctttaattac
     2761 ctaattcata aaataattat tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc
     2821 gcgaccattg accgttaaag gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac
     2881 cgataataat ttccgcgatg ccttttaaat cactgttttc gtgatacacc tcatcacgat
     2941 agataaacat gatcaagtcc gcatcctgct cgatagagcc agattcacgc aggtcggagt
     3001 tgaccggacg tttgtcggca cgttgttcca gagaacggtt caactgggac agcgccacca
     3061 ccggcacgtt cagttctttc gccagtgctt tcagcgagcg ggagatttct gcaatttcca
     3121 gcgtacggtt atcggaaagt gccggtacgc gcatcagttg caggtagtcg atcatgataa
     3181 gcccgatgcc gccgtgttca cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg
     3241 tcaggccgga ggagtcatcg atatagatat tgcgtttttc gagcaaaata cccatggtgc
     3301 cggaaatgcg cgcccagtct tcatcatcga gctgcccggt acggatttta gtctggtcaa
     3361 cgcgcgacag cgacgccaga gaacgcatca tgatctgttc tgatggcatc tccagcgaga
     3421 aaataagtac cggtttatcc tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg
     3481 ttgttttacc catcgacgga cgcgcggcga cgatgatcaa atccgacggc tgcaagccag
     3541 cggttttttt gttgagatca tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct
     3601 gctgaaataa ctgctcaata cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg
     3661 gcccttcgtc tttgttcgca cgactttcgg caattttaaa gacgcgggat tcagccagat
     3721 ccagcagatc ttcgctggta cgcccctgcg gatcaaaacc agcttcggca atctcattcg
     3781 caaccgagat catctcacgg acaacggcac gttcacgcac gatgtccgca taggaactga
     3841 tgttcgcagc acttggcgta ttttttgaca actctgccag ataagcaaaa ccaccgacgc
     3901 tatcgagttg cccctggcgt tccagcgatt ccgcaagggt aatcagatcg ataggactac
     3961 cgctttcctg caaacgcgcc atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat
     4021 cgtctgctac cacacgctcg gctacatcat cccagcgttc gttatccagc attaaaccgc
     4081 ccaacaccga ctgctccgct tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg
     4141 gatcgcgttc gcggggttca gcctgctgtt tgttgaaggg tttatttcct gccatagtga
     4201 atggagttac cgagataaag aatgggtcga aactttacca tatgaagcag accctgacga
     4261 tacgttctgg aggacacatg gcaacacgaa ttgaatttca caagcacggt ggcccggaag
     4321 tacttcaagc cgtagagttc actcctgccg atccggcgga gaatgaaatc caggtcgaaa
     4381 ataaagccat cggcatcaat tttatcgaca cgtatatccg cagcggcctt tacccgccgc
     4441 catcactacc cagcggatta ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg
     4501 gtgtaaagca tattaaggca ggcgatcgtg tagtctatgc gcagtcggcg ttaggcgctt
     4561 acagctctgt gcataacatt aatgcggata aagcggcgat tctgcctgcg gcaatttctt
     4621 ttgagcaagc tgcagcatct ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa
     4681 cctatgaaat taaacccgat gaacagttcc tgttccacgc agcagctggc ggcgttggct
     4741 tgattgcctg ccagtgggca aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg
     4801 cgcaaaaagc gcagagcgcg ctaaaagcgg gcgcgtggca ggttattaac tatcgtgaag
     4861 agaatctggt cgagcggtta aaagagatca ccggtggtaa aaaagtgcgc gtagtgtacg
     4921 attccgtggg cagagacacc tgggaacggt cgctggattg cctgcaacgc cgcggcttaa
     4981 tggtgagttt tggcaactca tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc
     5041 agaaaggctc gttgtatgtg acacgccctt ccctgcaagg ctatatcacc acgcgggagg
     5101 aattaaccga ggccagtaat gaactgttct ctttgattgc cagcggtgtg attaaggtcg
     5161 atgtcgccga gcagcagaaa tatccgctga aggatgcgca gcgtgcgcat gagattctgg
     5221 aaagccgcgc gacgcaaggt tccagcctgt tgattccata aaagaaatag ggcttccacc
     5281 tgggaagccc tttcttttta tagttcggct gtatgtaggg tacagcacga tgaatctgtt
     5341 agaggcgcaa tagtgacaga tttgattatc aattcctatt ttgttctaag gataaaacct
     5401 taggttgtga tcatccgcac aatcccttag taacgccagc ggtcataacg ctgatatttc
     5461 ggcacttttg gtgctttaat cgccttaata acccacacca ccgcaatcgc caggagtaac
     5521 cacggcagca acttaatcat caatgccagc ataccgccga ggaacataat ggccgtcgcc
     5581 acaaccagcg cggcgataat gcccagcaac gaaacgccgg tgaccatcaa catgacaaaa
     5641 aagccaatca caaaaagtag ttccagcatg atgctctccc aaatatgaaa tctcttgcag
     5701 gcattacaag aatcatgcca aaaataatct attgatttaa cagcaaaacg ccccgcgacg
     5761 gtgcgcaggg cgtggtgaat ttgactactt tttggtgaaa agttaacgct tatccgccac
     5821 cagtttgagc gcgtgttcca gcacgttaat gtctgcaccc gctttatggg cattttcact
     5881 taaataacgc cgccactgcc gcgcgccagg aataccctgg aacaagccca acatatgccg
     5941 ggtaatatgg ccaagatacg tcccctggct gagttcacgc tcaatgtacg gatacatggc
     6001 gcgcactacc gccaccggat cggcatcggt atccgaggaa ccaaagatct ctcggtctac
     6061 cgccgccaga atacccggat tctgatacgc ttcgcgcccg accatcacgc catccatatg
     6121 ttgcaaatgc gctttagcct cttccagcga cttgatacca ccgttaatcg acatcgtcag
     6181 atgcggaaag tcacgcttca gttgatacac acgcggataa tcgagcggcg ggatttcacg
     6241 gttttctttc gggcttaacc ccgaaagcca ggctttacgt gcgtggatga tgaacatctc
     6301 acactcgcct ttgccggaaa cggtgttgat gaaatcgcag agaaattcat agctgtcctg
     6361 gtcatcgatg ccaatacgcg ttttcaccgt caccggaatc gacaccacat cgcgcatcgc
     6421 tttcacgcag tcggcaacca gctgcgcatt acccatcaga cacgcaccaa acatgccgtt
     6481 ctgcacccgg tcagacgggc agccgacatt caggttgatc tcatcatatc cacgcgcttc
     6541 tgccagcttc gcacactgcg ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac
     6601 cggatgttct tcttcactgt acgccaggta atcaccttta ccgtgaataa tcgcccctgt
     6661 agtcaccatt tcggtataca gcaacgtatt gcgggaaagc agacgcaaga aatagcggca
     6721 atgtctgtcc gtccagtcga gcattggtgc aacgctaaac cgaccactcc agtgaacgtc
     6781 agttttttca ggcattgcgc tggtttggtt gattttttgc atttcagaat taccgtgcat
     6841 tttcaaatgt agagattatt ttatcgatat atcatgtggt tatgttattc agcatcactg
     6901 ttcaggaggc tcaataacgg ggtactatac cataacaaca ggaagcgcct gtctcattgc
     6961 aaaagaaaat tgagatcctc tcaaggcatg aagctctcac gaagtgaata tctaatctta
     7021 ttagatgtta gcgttgtgaa aagcaatgat aaacaataca gattcgacga taaataaaaa
     7081 tcacacatta aactccggtg atatctctcc ctgctaatag cactgataga gaaaaaaaga
     7141 acccaataaa cattgggttc ttttatgtaa tacctttcat accatcgaag aacttcacat
     7201 attatttctc cgatttaacc ccgaataaat cataaattaa tttagaagtg cctgtatata
     7261 ttttaatctt ttcctttgag tttgggtcaa acgacttagc aaagtcaatt aatttcggtg
     7321 cagcatctcg cattttgctt aaaatatcag gggcgagcgt tccttcattc accagatgtg
     7381 acatcttatc cagatagcca tcaaaattca tttctccccc atctgccaga cgcttcatct
     7441 ctcccaggta cttcttcata tcacgttgag ataattcttc aaactgagct ttcaggtagt
     7501 tatcattata ttcattaata ttcactcttc ctgcaaaatc tgagtagaca ctagaatatg
     7561 aaagggaaat taacagcgaa gcaaataatt ttaagctgtt gtcatcatgt tcctggcagg
     7621 cattaacaaa cgttaagaaa ccagaaccta ttgcttgcaa atgtatattc gctaaaggtt
     7681 cattatcttt agattcctca taaaatagct gaaccgtgga gggaagacca ttcttggttt
     7741 ctacatcaaa agtacatcgt tctatgagtg tcgcatcttt attatcctcg ggattacata
     7801 tactattaat cgcgaaatga aaaatagccc tgtcaacaac tgatgcaata aattcacgat
     7861 cgtttaattc ctcatcagta caatctccaa tatactcatt gacacacatt ttgagatcgt
     7921 ttatttgctt aattaacgtc acattttttt cacgtgcagt ccataaatta tgagtgattc
     7981 tttgaataaa taaatctatt tttgcaataa ttatatcccg atgacgca
//
LOCUS       DAZFKY010000195         8014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFKY010000195 DAZFKY010000000
VERSION     DAZFKY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..2132
                     /gene="iha"
                     /locus_tag="ACHKCL_004031"
     CDS             42..2132
                     /gene="iha"
                     /locus_tag="ACHKCL_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5764901.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2350..2500)
                     /locus_tag="ACHKCL_004032"
                     /pseudo
     CDS             complement(2350..2500)
                     /locus_tag="ACHKCL_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2727..3032)
                     /locus_tag="ACHKCL_004033"
                     /pseudo
     CDS             complement(2727..3032)
                     /locus_tag="ACHKCL_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3454..4029)
                     /locus_tag="ACHKCL_004034"
     CDS             complement(3454..4029)
                     /locus_tag="ACHKCL_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5764902.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4098..4676)
                     /gene="terD"
                     /locus_tag="ACHKCL_004035"
     CDS             complement(4098..4676)
                     /gene="terD"
                     /locus_tag="ACHKCL_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO5764903.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4725..5765)
                     /gene="terC"
                     /locus_tag="ACHKCL_004036"
     CDS             complement(4725..5765)
                     /gene="terC"
                     /locus_tag="ACHKCL_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO5764904.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5788..6243)
                     /locus_tag="ACHKCL_004037"
     CDS             complement(5788..6243)
                     /locus_tag="ACHKCL_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5764905.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6266..7423)
                     /locus_tag="ACHKCL_004038"
     CDS             complement(6266..7423)
                     /locus_tag="ACHKCL_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5764906.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7423..8004)
                     /locus_tag="ACHKCL_004039"
     CDS             complement(7423..8004)
                     /locus_tag="ACHKCL_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5764907.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2023 a         2046 c         2020 g         1925 t
ORIGIN      
        1 gtgtattgtc ttgccggtta acatgatcgg agattagtaa tatgcgaata accactctgg
       61 cttccgtagt cattccctgt ctcggatttt cagccagcag catagctgct gcagaggatg
      121 tgatgattgt ctcggcatcc ggctatgaga aaaagctgac taacgcagcc gccagtgttt
      181 ctgtgattag ccaggaggaa ttgcagtcca gccagtacca cgatctggcg gaggctctga
      241 gatcagtaga gggtgtggat gttgaaagtg gtacgggtaa aaccggaggg ctggaaatca
      301 gcatccgagg aatgccagcc agttacacgc tgatactgat tgatggtgtt cgtcagggcg
      361 gaagcagtga cgtgactccc aacggttttt ctgccatgaa taccgggttc atgccccctc
      421 tggccgccat tgagcgtatt gaggttatca gggggccgat gtccacactg tatggctctg
      481 atgcgatggg cggtgtggtg aatatcatta ccagaaagaa tgcagacaaa tggctctctt
      541 ccgtcaatgc agggctgaat ctgcaggaaa gcaacaaatg gggtaacagc agccagttta
      601 atttctggag cagtggtccc cttgtggatg attctgtcag cctgcaggta cgcggtagca
      661 cacaacagcg tcagggttca tcggtcacat cactgagcga tacagcagcc acgcgtattc
      721 cttatcccac ggagtcacag aattataatc ttggtgcacg tcttgactgg aaggcgtcgg
      781 agcaggatgt gctctggttt gatatggata ccacccggca gcgttatgat aaccgggatg
      841 ggcaactggg gagtctgacg gggggatatg accggaccct gcgctatgag cgaaacaaaa
      901 tttcagctgg ctatgatcat actttcacct tcggaacatg gaaatcgtat ctgaactgga
      961 acgagacaga aaataaaggt cgtgagcttg tacgcagtgt actgaagcgc gacaaatggg
     1021 ggcttgccgg tcagccgcgg gagcttaagg aatcgaacct tatcctgaat tcattactgc
     1081 ttacccctct gggagaatct catctggtta cggtgggggg cgagtttcag agctcgtcca
     1141 tgaaagacgg agttgtcctt gccagcacag gtgaaacttt ccggcagaaa agctggtcgg
     1201 tatttgctga ggatgagtgg catctcacgg atgcacttgc gctgactgcg ggcagccgct
     1261 atgaacatca tgagcaattc gggggacact tcagtccgcg tgcatatctg gtctgggatg
     1321 tggcagatgc ctggacgctg aaaggcggtg tgaccacggg atataaggca cccagaatgg
     1381 ggcagctaca taaagggatt agtggtgtgt ccgggcaggg aaaaacaaat ctacttggta
     1441 accccgacct gaagccggaa gagagcgtca gttatgaggc tggggtgtat tacgataacc
     1501 ccgccggtct gaatgccaat gtcacaggtt ttatgactga cttctccaac aagattgtct
     1561 cttattccat aaatgataac accaatagct atgtaaacag cggaaaggcc cggttgcacg
     1621 gtgtggaatt tgccggcaca ttgccgctgt ggtcagagga tgtcacgctg tcactgaatt
     1681 acacctggac ccgaagtgaa caacgtgatg gtgataacaa aggtgcgccg ctgagttata
     1741 cccctgaaca catggtgaat gcgaaactga actggcagat caccgaagag gtggcatcat
     1801 ggctgggtgc ccgttatcgc gggaaaacac cacgtttcac ccagaattat tcgtcactga
     1861 gcgctgtaca gaagaaagtg tatgatgaga aaggagaata cctgaaagcc tggacggtgg
     1921 tggatgcagg tctgtcgtgg aagatgacgg atgccctgac gctgaatgct gcggtgaata
     1981 acctgctcaa caaggattac agtgacgtga gcctgtacag tgccggtaag agtacgctgt
     2041 atgccggtga ttacttccag acgggatcat caacaacagg atatgtgata cctgagcgaa
     2101 attactggat gtcgctgaac tatcagttct gataataaca aaacgctatc actgacggta
     2161 gaatacgttg ccactgcaac tcctggcgga acagtggcaa cgtattaggt taagtgcatt
     2221 tccgatccgc taatgagatt tcgttaccaa caactaatat cgtcacagga aatgcacgga
     2281 ttatttttaa cttatcattt acatacttgt ccagagtgta agcgcaccgt gaaggacgtg
     2341 gggtaaaaat tagtttacag agagagtgac gttccagggc aacaactctt tcatgcggtt
     2401 ggcaggccag gtgttgatta cactgatcac gtggcgtagc cacgtttccg gctcgattcc
     2461 gttaagtttt gcagctaccg atcaggctgt acatcactgc cgcactatcg ctcgtcatct
     2521 caaagtcctg tctcgtcagc aggaaggtat cattctctcc cgccattttt ccaggggccc
     2581 ggtcagataa gtccctttgt ctatcgctga ctcctgactc ataacccggt tagcagaatg
     2641 cagggtcacc acctcgccac gaccaaatcc aaataagtca attgcacctt ctcaatcgcc
     2701 attttgtcag taagcgtaca gcctcaactg atggtatctt caccatcaat gacaacggtg
     2761 atcgcaattt tactgacgtt cgccggaaca cgatccagtg ctatctcaat gctggcctgc
     2821 tgcgaaccgg taacgagcct gactgccccc tcaggagaag acaaattatt ataaaagata
     2881 aagtcagaat cgccactgac ctttccctga gcattaagca tgaacaggca ggtatcgggt
     2941 tcgcctttaa agccggattt ggcagggtac tgaagattca gcctgatcgt agattgctga
     3001 aggggtatgt tttgttcgga ttgtaaattc atattaactc tcctgatttt tgattattat
     3061 taatgcgcag cgtttatata tgttccctag gcttagttct ggacgctgga tattcggtga
     3121 ggcgtaaata tggtatgaca ccatttttca taacgctgaa gtttctatac ctgttgaatt
     3181 tgaattttca ttgaccgggt atcttatttt ccagggcccg ttccttcata agtcgcaaaa
     3241 gtaacatata tccgaagggc atgctgttga tatcagacac ggaatactgg ctttaaccag
     3301 caaccacaga taaacccggg gcctgcataa aaggttcatg ccagaattaa catagctctg
     3361 ctttctgccc ccccctttcc catattcacc cgttgatagc ggatcgatac ccaaaaaaaa
     3421 ccccggcatt gccggggtca gactcagcgt cttttagaca ttgacgccgt gctgggaagc
     3481 aagcgctgac agaccaccgg caaaaccctg gccaacagct ttaaacttcc actcagtacc
     3541 atggcgatac agttcaccga agaccattgc ggtttcggtt gaggcgtctt cagacagatc
     3601 gaaacgggca atttccgtcc cgttgtcgtt gttgtaaacg cgcatgaagc tgttgctcac
     3661 catgccgaag ttttgtttac gcgcttctgc atcatagatg gtaacggcaa ataccagttt
     3721 tttgatgtct gctgagactt tggtcagatc gattttgacc tgctcatcgt cgccgtcgcc
     3781 ttcaccggta cggttgtcgc cctggtgctc tactgcgcca tcagggctgg ttttattatt
     3841 gaagaaaatg aaatgggcat ctgacagtac tttaccgtct tcacctactg cgaatacgga
     3901 agcgtccaga tcaaaaccct gaccatcggt tacacgggca tcccagccca ggccaaccat
     3961 agcgacattc atggttggtg cttctttggt cagagatacg ttgccgcctt ttacgagaga
     4021 aactgccatt tttagctcct gcaaacagtt gaatgaggct gaattaacag ccccagatat
     4081 gaaaagttac ttttcgatca ggacgcgtta atgccgtact gagcacatac agatgccaga
     4141 ccaccagcat aaccctggcc tactgcgcgg aatttccact caccattgtg gcgatacagc
     4201 tcgccgaaca gcatggcagt ctcagtggac gcatcttcgg tcagatcgta gcgagcgact
     4261 tcagtctggt tatcgtcatt aaccagacga ataaacgcac cggatacctg accaaagctc
     4321 tggcgacgag cctgagcatc gtggatggtc acaacgaaga tgatcttgtc aacttcagac
     4381 gggacggcgt ccagtttaat tttcagcgat tcatcatcac catcgccctc accggtgcgg
     4441 ttatcgccgg tgtgcgttac ggaaccgtcg gatgacgtca ggttgttata gaagatgaaa
     4501 tctgaatcgc cgcgcacttt gccgtttgag gccagcagga atgctgaagc atccaggtca
     4561 aagtcctgac cgtctgttga acgcgcatcc cagccaaggc ccaccaggac atttttcatt
     4621 gacggagctg ctttactcag ggagacgttc ccgcctttgg aaagagaaac actcataaaa
     4681 taacctcttc gattagtaat tgttcaggtt aacacttaag gggattaact ccccttttcc
     4741 tcagattcag gtttgcccgg gaacatgacg cttgcgagaa tgcccagcgc cagtacaccc
     4801 agtacaacat acaggctggt tgttgccgcg atgctgtaac catgatgcca gatgtgatcg
     4861 gtcgcattca ggccgagttt tgccgcgatg aagaacagca gcacgataac ggccttctcc
     4921 agatgaacca ggtactgttt cagtgcctca aggacaaaat acagagtacg cagacccagg
     4981 atagcaaaca tcatggcact atagacgata agcggttcac gactgacggc aatgattgcc
     5041 ggtaccgagt caaacgcgaa cattacgtcc gagagttcaa ccacagccac acacaggaac
     5101 agcggggtcg catacagcgc cgctttttta ccacggccaa tggtgacatc gctgttttct
     5161 ggtttcgcca gttcagcatc cacttccttc tggttaagta ggaaggcatg ccctctgagc
     5221 ttcggccaga tagggaagaa gcgtttaacc atacggtaag ccagatgctg ggagtaatcc
     5281 tcaatttcat catcgtcatc accgctttta agcatcatga ccgccgtcca ggcaacgata
     5341 atagcgaaga caacttcaac gtacggcccc agactcagca ggctcgtacc gatggcgaca
     5401 aagatgcccc tgaagacgat ggcaccaatg atcccccagt agagaacacg gtggcgataa
     5461 cgatccggaa cggcgaacca ggagaagatt gccatcatga cgaacaggtt atcgaccgac
     5521 agcactttct ccagcgcata acccgtgaca aacagactgg caacctcagc accgtggtgg
     5581 atatagagga aaccggcaaa tgccatcgca accacaaccc agaatacgga ccagagcgca
     5641 gcactcttca gcgagattgg cttgtcatca cggtgcataa acaggtcaat aaagatggca
     5701 ccgactgaaa gcgcaataaa aacaatgacc gtttcagtcg ggaagccgat gtgtgtggaa
     5761 accatatttt acccttaggg atgtggatca gaggccaaat tcagccgggt cgaagcccaa
     5821 cgcgatagcg atctcgcggg aggccagttt ctcgtcctga tcgaagttac cgtcactttt
     5881 cgcaacggca ataccaacac gtaaggccag ctgagcggcc tcaggctgat ctttcagcgc
     5941 caggatgtat ttcatggttt cgcccttgcc aatttcaaca tcgaaatcga agcttgaaac
     6001 cagtttattg aagaactcaa tcacctcatt ggtatcgaag accttcagct cttctgaaga
     6061 gcgcagaaag cccatcattt tctgcttttc ttccgaactt acgccgtcac tcgatacggc
     6121 aatacgggca catacagcaa cggtgccctg catgaatttt ttgtttttga aacggccaac
     6181 ctggcgggtc agttcgtcac ggcctgagtt aatggcacct ttaactttgt cgaaaaagct
     6241 catacctgtt tcctttgttg ctgtgttatt tagaaccggc gctccagcga aatccccagc
     6301 caaatgcccg gtccatttcg tcctggcctt tgaagtactg gttaattcgc tcgactttaa
     6361 ttgcgccgtt ttcgtttacg agtctggcaa tggcgcacaa tgtgcgacgg ttctcacctt
     6421 cggtcaggcg ggtctcaatg ggcggttgat ccggcacatg aatggtgact acaccatcag
     6481 tcttgtccca gctgggaaca ccttcataaa taaaggcgta aatcagcact tcgcggatat
     6541 gcttccattc acgtccattg atatgcagcc actcgccgtc tgatacatca cccgtccggt
     6601 catcgccctt gagctgaaca tacggttcat cgcgataatc accgaatgca ttaccgagcg
     6661 cctgaatgac cgatttatac ccgtcctgaa gctcaacaaa ggcccccaga tccaggtcga
     6721 ttcctttgga gccgaacata cctgcaaagc ctgatttgct actgccccgg tgccagttca
     6781 ggttgatccg gatttcaccg aagttatccc gtttggtcag gctgattgca ggcttctctt
     6841 ttgtcagcga taccttgctc aggctgattt ttgtctcaac tggcggaggg gttacaaccg
     6901 gaggagttgg ggctgcggga gccggttcgt cggcaatatt tacgccgaaa tgctctgcca
     6961 gcggtttaag tccaccgttg aacccctgcg caacgaagcg gaatttccag tcattattac
     7021 gacggtaaaa ttcccccaga attaaggctg cttcctgacg gccgctcagt tcgacactgc
     7081 cgctaaccag gccggtagcc ccttgctcca cgtcgatgga aagattacga agaccggaca
     7141 ccgtctgccc accgtcacag gtcacggtga acgcaatttt ctggacatca ggtttcagcc
     7201 ggttgagcgc gacagtaaag gttgagtact ggccttcact gaccaggctg acggtaccat
     7261 catcgttacg tggctgacca taaaacacca tgtccgcatc accctgcacc ttcccgtcgg
     7321 catacaggcg aaacgccgaa gcatccacct ggccgcccga ggtgatccgg acccgcaact
     7381 cctgggctgg aacgggggca ttgccccccg gcgtcaggtt cattagcgaa taaccgctga
     7441 aacgatatcg gagtgcatat cgtcaattgt gcggccgcga caggcatggc caagggcggt
     7501 aaagtcccag ttgccgttat tacggcgcag cgaagaaata acgatcccgg tatgtgagcc
     7561 ctgctcagtc agcttgtagc gagccagctc tttgccggtt tgatcgacaa cgcggcagaa
     7621 agcgttttcg acgtcattga acgactgacc acggaagctg tttaccgtaa acgccaggta
     7681 ttcaacgttt gcaggcaggc gggagaggtt gaccttaatc acttcatcgt caccgtcacc
     7741 ctcaccggtc aggttatcgc cactgtgcac aacagcgccg caggtggatt tcagtttgcg
     7801 gaaccagatg gtgtcaattg ttttacctgt gctgtccatt aatacacagc cagcatcgag
     7861 atcgattgag tcgttgcctc caaacaatcc tccaagaaga ccttttttct taaccggatc
     7921 ccaaccgaga ccgaaatgaa gctggcttaa tgcagatgat tctttgctga gtgataccgt
     7981 ctggttctta actaatgaaa ccatgtttta ttcc
//
LOCUS       DAZFKY010000196         7948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFKY010000196 DAZFKY010000000
VERSION     DAZFKY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..234
                     /gene="yafQ"
                     /locus_tag="ACHKCL_004040"
     CDS             <1..234
                     /gene="yafQ"
                     /locus_tag="ACHKCL_004040"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO5764908.1"
                     /translation="VKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGY
                     RDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            390..1130
                     /gene="dpaA"
                     /locus_tag="ACHKCL_004041"
     CDS             390..1130
                     /gene="dpaA"
                     /locus_tag="ACHKCL_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5764909.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1101..1868)
                     /locus_tag="ACHKCL_004042"
     CDS             complement(1101..1868)
                     /locus_tag="ACHKCL_004042"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5764910.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1973..2551)
                     /gene="lpcA"
                     /locus_tag="ACHKCL_004043"
     CDS             complement(1973..2551)
                     /gene="lpcA"
                     /locus_tag="ACHKCL_004043"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5764911.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2791..5235
                     /gene="fadE"
                     /locus_tag="ACHKCL_004044"
     CDS             2791..5235
                     /gene="fadE"
                     /locus_tag="ACHKCL_004044"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5764912.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5278..5724)
                     /gene="ivy"
                     /locus_tag="ACHKCL_004045"
     CDS             complement(5278..5724)
                     /gene="ivy"
                     /locus_tag="ACHKCL_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5764913.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5905..6675
                     /gene="yafV"
                     /locus_tag="ACHKCL_004046"
     CDS             5905..6675
                     /gene="yafV"
                     /locus_tag="ACHKCL_004046"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5764914.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(6793..>7948)
                     /locus_tag="ACHKCL_004047"
     CDS             complement(6793..>7948)
                     /locus_tag="ACHKCL_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO5764915.1"
                     /translation="LDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEWQEIEDF
                     GHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITDGEIIAI
                     DGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLNLLDLKK
                     NLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNYKGDSFS
                     TQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAEGVSIRY
                     YISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISGIKKMAL
                     NLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1912 a         2008 c         1971 g         2057 t
ORIGIN      
        1 gtaaaacttg cacaaaagcg tcataaggat atgaataaat tgaaatatct tatgacgctt
       61 cttatcaata atactttacc gcttccagct gtttataaag accacccgct gcaaggttca
      121 tggaaaggtt atcgcgatgc tcatgtcgaa ccggactgga tcctgattta caaacttacc
      181 gataaacttt tacgatttga gagaactgga actcacgcgg cgctctttgg gtaaagcccg
      241 ctgccgatga aatttgtaaa ccctgcgccg acaggcttgc gtaaaactct tttggtgata
      301 atgtgagcct tcccaatcca gactattccg atttgggatc cattttgccg tcctggcgtg
      361 tgtaaccgtt ttatcaagga ataagcagta tgcgtaaaat cgcattaatt cttgcgatgt
      421 tgttgatccc gtgcgtttcg tttgccggtt tgctgggcag cagtagctcg accacgccag
      481 tgagcaaaga gtataagcag cagttgatgg gatcccctgt ctacatccag atcttcaagg
      541 aagaacgtac gctcgatctc tacgtcaaaa tgggcgagca atatcaactg ctcgacagct
      601 ataaaatctg taaatattcc ggcggcttag ggccaaaaca gcgtcagggc gatttcaaaa
      661 gcccggaagg gttttatagc gtccagcgta atcagttaaa accagacagc cgttactaca
      721 aagcgattaa tattggtttc cccaatgcct atgaccgtgc gcatggttat gaagggaaat
      781 acctgatgat ccacggcgat tgtgtttcca tcggctgcta cgcgatgacc aatcagggca
      841 ttgacgagat tttccagttc gttactggcg cactggtatt tggtcagccg agcgtgcaag
      901 tgagcattta cccgttccgc atgaccgacg ccaatatgaa gcgccataaa tattccaact
      961 ttaaggactt ctgggagcaa ctgaagccag gctacgacta ctttgagcaa acccgtaagc
     1021 caccgaccgt ttctgtggtc aatggccgtt acgtggtcag caagccgtta agccacgaag
     1081 ttgtgcaacc acaactggca tcaaactaca cgctccccga ggcaaaataa gcgccattcg
     1141 cctggcataa tcttttgcca ggtttcattg cccgtcagcg gctgtgtcgc aatcaccgtg
     1201 accacatcat tcggtgtggt ctgcgagctg aagtcgattt ccacatcctg atccagcaac
     1261 gttgccacgc caaacggcgc gcggcgggtg atccagtgca gattggtcga gcaatacgcc
     1321 attacatagc gcccatccga aagcagcatg ttgaaaacgc ccttctgccg cagttcatcc
     1381 gccagtgagg cgatatattt aaataccgcc gccatattgc ccggcgtacg cggataacgc
     1441 tgcgttaatt tatacagcag ccagcaaaag gctttttcgc tgtcggtttt acctaccggg
     1501 cggaagttgc cggtttccag tgatttatag cccgtcagtt gtccgttatg ggcataagtc
     1561 cagttacgtc cccataactc gcgggtaaac gggtgagtat tttccaacgc cacctcgccc
     1621 cgatttgcct ggcgaatatg agccacaacc gaacaggatt ttatcgggta gtcctgaaca
     1681 agtttggcaa tgggggaatt aaagctgggt tgtggatctt taaatgtgcg acagccttta
     1741 ccttcgtaaa aggtaatgcc ccagccatct ttatgtggcc cggttccacc accacgctgc
     1801 accagcccgg tgaaactaaa gcaaatatcg gtagggacgt tggcgctcat cccgagcaat
     1861 tcgcacatcg gtaaccccca caacagcggg agcgcatggc ccccggcgaa gattgactta
     1921 agaattttca ggccggatgc ggcgtaaacg cattattcgg cctacgccag acttacttaa
     1981 ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga atttcctgaa
     2041 tgcggtcggc ataaccaaaa tgtggaacgc gaatttcaat atccgccgta ccagccattt
     2101 tgccgccatc tttgccggtc agggtaatca ctttcattcc cttctcacgc gccgccgcga
     2161 tcgctttgat cacgtttgca gagttaccag acgtggagat ccccagcagt acatcgcctt
     2221 cgcggcctac cgcttcaaca tagcgggaga aaatatcatt gaaaccaaaa tcgttaccga
     2281 cgcaggaaat atggctgacg tcagagatgg caatcgccgg gtagcccgga cggttttcac
     2341 ggtagcgacc ggtcagctct tcggcaaagt gcatggcgtc gcaatgggag ccgccgttgc
     2401 cgcaggaaag caccttgcca ccggctttaa agctgtctgc taacaggacc gccgctcgct
     2461 gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt
     2521 tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag
     2581 acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca
     2641 ggactttttg ccctgaagtg tagataaaaa cagcaacaat gtgagcttcg ttgtaattat
     2701 attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc
     2761 agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacagtt
     2821 gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg
     2881 ctcgcctgga cagctgccct cggcgtcgct ggtctgtggt cggcgtgggt actggtgcct
     2941 ctggccatta tcctcgtgcc gtttaacttt gcacctatgc gtaagtcgat gatttccgcg
     3001 ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg
     3061 attgatgcgg gtaccacctg gtgggaaggc gacttgttcc agggcaagcc ggactggaaa
     3121 aagctgcata actatccgca gccgcgcctg accgcagaag agcaagcgtt tctcgacggc
     3181 ccggtagaag aagcctgccg gatggcgaat gatttccaga tcacccatga actggcggat
     3241 ctgccgccgg agttgtgggc gtatctgaaa gagcatcgct tcttcgcgat gatcatcaaa
     3301 aaagagtacg gcggcctgga gttttcagct tatgcccagt ctcgtgtgct gcaaaaactc
     3361 tccggcgtca gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggccccggc
     3421 gagctgctgc aacattacgg cactgacgag cagaaagatc actatctgcc gcgtctggcg
     3481 cgtggtcagg agatcccttg ctttgcactg accagcccgg aagcgggttc cgatgcgggc
     3541 gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg
     3601 atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac tgtgcttggg
     3661 ctggcgttta aactctccga cccggagaaa ttactcggcg gtgcagaaga tttaggtatt
     3721 acctgtgcgc tgatcccgac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg
     3781 ctgaatgtgc cgttccagaa cggaccgacg ctcggtaaag atgtcttcgt accgatcgac
     3841 tacattatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc
     3901 tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg
     3961 ctggcaacgg gcgcgtatgc tcacattcgc cgtcagttca aaatctctat cggtaagatg
     4021 gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat ggacgctgcg
     4081 gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc
     4141 gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat ggatattacc
     4201 ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca
     4261 ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga
     4321 caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat
     4381 gacgtcaacg tgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa
     4441 gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat
     4501 gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct
     4561 gatgtctcga tggcagtgct gggcggcagc ctgaaacgtc gtgagcgtat ctcggcacgt
     4621 ctgggtgata ttttaagcca gctttacctc gcctctgccg ttctgaagcg ttatgacgac
     4681 gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga tgcgctgtat
     4741 caggcagaac aggcgatgga cgatttactg caaaacttcc cgaaccgcgt ggttgccggg
     4801 ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc tgacaagctg
     4861 gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgctcacg tattggtcgc
     4921 ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga ggcgttggtg
     4981 gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctcgg taaaaatctg
     5041 ccatttaccc gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa
     5101 gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat taacgttgat
     5161 gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa
     5221 gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta
     5281 tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac
     5341 cgttttacca tcaatcgaaa gcgcatcgtt cacattcaac caggtcagtt tctcttgcga
     5401 cgttttctca tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat
     5461 cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt
     5521 ctcatctccc aacgttacgg tttgtgcagg ggtataagta ccgcctttca tcacccaggc
     5581 aggcagctta tgcccttgca ccatctgatt aaatgcagct ttggtggttt cgccctttgc
     5641 aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga ccagtgcggc
     5701 gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca tgttattaac
     5761 ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt
     5821 tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc
     5881 cttcgcataa aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca
     5941 ctggtgtgga tggatggtcc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt
     6001 accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc catggaagcg
     6061 gctgcttcgt cgctagcaca agatgacgta gtgaactgga tgacagccaa agcgcagcag
     6121 tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc
     6181 tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc
     6241 atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc
     6301 ggctggcgta ttttgccgtt ggtgtgctac gacttacgtt ttccggtgtg gtcgcgcaat
     6361 ctcaacgatt atgacctcgc catctacgtt gccaactggc ctgctccgcg ctctctgcac
     6421 tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat
     6481 cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaatcca
     6541 caaggcgaga ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgcggagctg
     6601 tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga tgcggatgag
     6661 tttaggttgc ggtgagtggg ggctgtattt agggcgggga attcagaaag ggatgtttag
     6721 tgtgctgagc gagagttaaa taataaaaaa ctgggaagat ttaataatcc ccccgcaggg
     6781 caagagcatg aattaaatct gtgttaaatt tttcattttg tcagttcgaa acccaagctc
     6841 tgagcaggat gcaagcactt cttctatata acatgtgttt aacgcaacct tctttctttt
     6901 cctcttcacc ccacccttaa tgtctttgca atctcttaat aaattcagtg ccatcttctt
     6961 tattccagat attatttcgg ctgcgtttcc tcttcttatc cggctggcat cttcattcat
     7021 ttttacatct aacacccaat gaagactgtg ctcgatcagc cagtgcgctc tgatagcatg
     7081 tgcaaattct ttagcatcca tatcctttga tgaaatataa tatcggatgc ttacaccttc
     7141 tgctgattta tcttctttct tctgcctgaa tgacaatgct acacaaagct tttttaatcc
     7201 cttccattcg aattcaaaat cacaaaattc aggcgttacg ttactgacaa tatgcaaacg
     7261 tgtttctttt cttccatgac ttatctcctg cgtactaaac gaatcgcctt tataattaga
     7321 aaacacattt acaggaaatt tttcctcgaa tgcatgatgt aatttcccct gattgccttt
     7381 tactgccaga agataatctg cttttttatc tttgatcttc gaagcgatat ctttctgaca
     7441 gcccatagca tcaatggtta tcaaattttt ctttaaatcc agtaggttaa gcaactctgg
     7501 aatggctgta atctcattac ttttggcttc cgtttttacc tgccccagta caacaccatt
     7561 ttcgttcgag aatgcactca ccatatggat tgctcctttt ctttttccct tatcaaagga
     7621 gcctcttatg gtctttccat ctattgctat aatttcacca tcagtgattt catggcactc
     7681 ctgcatccat tcaataaata tcttttcaaa ggccaaactg tcaatgttac tcacaacgcg
     7741 tgcaatggtg tcatcgaccg gaatgccatt atcaaaatca ccatatttct ttagccattc
     7801 aagtctttca tgtccaaaat cttcaatttc ctgccactca tcggcacctg caattactgc
     7861 acatacggtg agaaacaaaa tatcagataa tttatgttta acctttcctt gttgtcgtat
     7921 atcagggatc actgaaatat aatcaagc
//
LOCUS       DAZFKY010000197         7934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFKY010000197 DAZFKY010000000
VERSION     DAZFKY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(107..1594)
                     /gene="yeeO"
                     /locus_tag="ACHKCL_004048"
                     /pseudo
     CDS             complement(107..1594)
                     /gene="yeeO"
                     /locus_tag="ACHKCL_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1755..1830
                     /locus_tag="ACHKCL_004049"
     tRNA            1755..1830
                     /locus_tag="ACHKCL_004049"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1788..1790,aa:Asn,seq:gtt)
     gene            complement(1868..2818)
                     /gene="cbl"
                     /locus_tag="ACHKCL_004050"
     CDS             complement(1868..2818)
                     /gene="cbl"
                     /locus_tag="ACHKCL_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO5764916.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2920..3837)
                     /gene="nac"
                     /locus_tag="ACHKCL_004051"
     CDS             complement(2920..3837)
                     /gene="nac"
                     /locus_tag="ACHKCL_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5764917.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4163..4238
                     /locus_tag="ACHKCL_004052"
     tRNA            4163..4238
                     /locus_tag="ACHKCL_004052"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4196..4198,aa:Asn,seq:gtt)
     gene            complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="ACHKCL_004053"
     CDS             complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="ACHKCL_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5764918.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="ACHKCL_004054"
     CDS             complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="ACHKCL_004054"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5764919.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="ACHKCL_004055"
     CDS             complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="ACHKCL_004055"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5764920.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7122..7667)
                     /gene="cobU"
                     /locus_tag="ACHKCL_004056"
     CDS             complement(7122..7667)
                     /gene="cobU"
                     /locus_tag="ACHKCL_004056"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5764921.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7819..>7934)
                     /locus_tag="ACHKCL_004057"
     CDS             complement(7819..>7934)
                     /locus_tag="ACHKCL_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO5764922.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2189 a         2158 c         1721 g         1866 t
ORIGIN      
        1 agaggaatcg tgagaacgag gcgaatatta gcgatgccca cccacaatgt caaagcctgt
       61 tttttaaatt tgaaatcgtt tgctgaaata atctgcattt tgtcgtttat tccgacacag
      121 ctggcttttt ttcacacttt tgcggttcgg gtcgagggta tttccacagc caacgtccag
      181 taaccattcg ccagtaaaac agcacggccc gcacagccca gtcggcaaac attcccatcc
      241 agacaccaac cacaccccag ccaagcatga tgcccagcac ataaccgacg acaacccgac
      301 aaccccacat gctcaacata gaaacccaca tagcgtaacg ggcgtcacga gcccccttaa
      361 atccagcggg tagcacccat gaagcggacc aaataggcat aaataaagca tttagccaaa
      421 tcagaatcac aacgacatgt ttaacctgtg gatcctaggt gtaaaacgat gccataaccc
      481 cggcaaaggg agccgttagc caggcgatgg ccgttaatcc aagagtggtc agccaaaaca
      541 catgccgcaa ctgaatctct gcttgcgcta tctgccctac ccccaacctt cggcctgtaa
      601 tgatcgtaga agcagaaccg agcgcacttc cgggtaagtt gataagagcc gcaattgaaa
      661 acgcgataaa atttccggca ataacactgg tccccattcc ggcaacgaac atttgggtta
      721 ataaccgacc actggtaaat aacactgatt cgacactcgc tggaatacca atccccatga
      781 cttcccagat aatgctaaaa ttcagcggtt taaaatagct ctttaacgaa atccttagcg
      841 caggattaaa accaatcgcc agcacccaca aaattgcaac tgcgccaata taacgagaaa
      901 tggttaaacc caacccagcc ccgacaaatc ccagtcccgg ccaggagaaa aggccgtaaa
      961 tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc agtagcggta
     1021 ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat tagagtgatg gcagctgctg
     1081 gataactgag taccgtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg
     1141 catcacccgc gacgaaatca ataatttgct cgccaaaatg atgaataagc gttgccaaca
     1201 gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc
     1261 gatcccgctt accgagacta aatgccacaa cgacagtagt accaagatcg atagcagcaa
     1321 aaaaagccat aatgaccata ttgaagctgt ccgccaatcc cacgccggcc atcgcatctt
     1381 ttcccagcca gctgaccaga aaagtgctca gaacccccat caacaggaca caggcattct
     1441 ccatgaagat aggaacagca aacggggtta tctcgcgcca gaacaacact ttgtagctct
     1501 tgcgtttagc gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca
     1561 aagccgacct taattgcaga aagtgaaacc acatttcaaa taatgaggga gaatcagcaa
     1621 agctgcaaag attttcgcca tcaaattgtc tgcaaatgca acaaactgtt gatagaaacg
     1681 gcaaacagtt gggaaaatta aaaatcgggt ttgacaaaag atttttcgcc gttaagatgt
     1741 gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     1801 tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg
     1861 ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc tgccgcttga
     1921 tatcctcaac tgaaagtcct gcattgcaaa gttccagaaa gcgccagaca tagttacgct
     1981 gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat
     2041 ccaggcggat taaattcttt tcctcttgtt caccgctgga ttgttcggca actaatccga
     2101 tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgcgca cttaatataa
     2161 tatctgccag caaacctttg cgggcaaatg cgtcatcaat acgtgagcgc cccgtaatcc
     2221 cctgtcggta agtgattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt
     2281 gcgtcaaggg atgatcgtgt ggaacaagca aactatggtg ccaacgaaac cacgggaagg
     2341 cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcaccat
     2401 tatgcaacaa cgtcgcaatt tcctgtggcg tcccctggat tagctcgagc cgaacctccg
     2461 ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg
     2521 tcgttgcaat agtgagaacg ccagacgtat cgttggtaaa caggtctgca agccgacgaa
     2581 cattactggc ttcattgaga atacgttctg caatgaccag taatgctttg cccggttcag
     2641 tcatgcccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt tcatcctcca
     2701 gttcccgaat atgacggctg acgcctgact gtgaggtaaa aagcatattc gcaacctctg
     2761 tcaggttgta atcctgacgt gcagcttcgc ggattatctt tagttgttgg aaattcacgg
     2821 taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaacgcccg gcataacaaa
     2881 taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg
     2941 ccttttttcc atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc
     3001 ctgtggcgat aagttcgctc tggcgggtaa atttaatgac aaagagagac tcatggaagg
     3061 cgtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg cggccgattc
     3121 gggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtag caatagactc
     3181 aatttcacca atgacttttg ccgtgagccg ccgtagggaa aacgcctcat caacacgaag
     3241 tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgtgcaatag cattaacatc
     3301 aacgctttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc
     3361 ctgactggat acaccagcca caggggaatg ctcataaatc accgccatat cgagttggtg
     3421 atttatcagt ttttcgttaa gcactgcacc gctattttca tgaagataga taacgacctc
     3481 cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc
     3541 tggcgcaaag ccaatcgaga cttgccccga taatgcctga ccaacgttat gcaccgccag
     3601 ttgggcctgc tcacactgac gtaaaatggc ccgcgcatgg gtatagagaa tttttccggc
     3661 gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagt tgttgattta actcaccttc
     3721 gagtgtggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg
     3781 agtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt
     3841 gcctccggtt tttgagaatc ggcccaagtg ccgccattac ttacaaccag attgcaagat
     3901 gcttgccagt tttattttgg cgttgatgta caagctaacc aactgtcaaa taagagatta
     3961 tgatagattc gtcattggct cttttcacca gcagtcgcgt tcccctgccc tataaaagga
     4021 gggtatgcac cacgatggtt cattaccaat aagattgaaa tctcatcact ttgttgaaat
     4081 tgacagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
     4141 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     4201 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttct
     4261 agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg aaccagaact
     4321 aatttgcaca ggcattcccg atcgacgttg caacgcagca tttgcgcgat tcacatcaac
     4381 ttcttgcccg ttgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     4441 agactcatat tcagcacgat tgcgcgacag cggctcatga acttccagcc actttgaacc
     4501 atccggttcc gttgtgtatt tcactggctg atcaataatt tgcacacgag tacctacagg
     4561 aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc cctgacttac
     4621 ccggagccca ataccaaaat tggcattggt accgtggatg gcatatagcc tgccaatata
     4681 aatcgcgtac agccccatgg gattatcggg ccccgcagga acaaatgcgg gcaaactctc
     4741 ccctcgtttc gcatattcgc gccgggtgtt cggcgttggc gtccaggttg gtgcttcttg
     4801 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     4861 aataggaaag acttccacag tattactgtc tggtgggtag taataaagac gcatctcagc
     4921 gacgttaacc acaatccctt tacgaacagt gacgggcaaa atcagttgct gcggaatggt
     4981 gagttgcgaa cctgacttcg gtaagaagac atcagcgccc ggattcgctt ccagcatgtt
     5041 acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg
     5101 aacagttaca gtaagcgact gccccactaa acggctaccc tctggaggta atggataagt
     5161 taccgccagg ctagtatggc tggcaaaaag cagagcaaat gagcaaagaa tatttacacg
     5221 acgcatcatg tccctttcct aagtcgcgaa agctatcggt taagtatagc ttttatcaga
     5281 cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca attttactgg
     5341 cagcaagttc gcccatgttg ttgtatatcg cacaagcagc ttcgatgatg ggcatcgcca
     5401 gagctgcgcc actcccctca cctaaacgca tatccatatt gagaaaaggc tccagcccca
     5461 aatgcgacag cgctatacgc gccccttttt ctgccgacag gtgagaagga atgagatacg
     5521 gtttgattgc aggagacatc tggcaggctg cgagcgcagc agcataagaa agaaatccat
     5581 ccagcaacac gggtaaaccg caggaagcag cgcctaacat cacgccagcc attccgacca
     5641 aatcaaatcc acccactttt gccagaacat taacgccatc ctgcggattt ggctgattca
     5701 acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     5761 cgccaatccc aaccacttct tcaggatccc ggccagtgat tgtgctgact attgctgctg
     5821 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtgaca ccgttttttg
     5881 ccagctcccg cgtataacat atgacgtcca aaagtaactt ttcagcctga cggcgactca
     5941 ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac acgcatgttg ataagcccgg
     6001 ggataggctc agcagtatca ataccaacat caattacgtg gacgttagcg cccgcttgtg
     6061 ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga atggctgtca
     6121 cttcttttgg ggaaatagcg accccttcct cccagacgcc gtgatcggca cacataacca
     6181 gtaccgcttt tttgcccaca tgcggtatgc cattcaaccc cggcattccc gccagttgta
     6241 cgccaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg tcaatatgcc
     6301 gttgtgcacg cgacatagcg gcaggatcga tggcagggat cgtattcagt aaattggcaa
     6361 gtgtttgcat gtcacgtctc tttatagcag tgccagcagg aagaccagtt caccaagctc
     6421 tatggctgct cccagcgtat cgccggtttg cccgcccagc gtacgtttga gaagctgacc
     6481 gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccggcaa
     6541 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     6601 tttgccaatg aacacattgc caagcccttc ttcccgggca taacgatgac gatacatcaa
     6661 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     6721 gccacgcaga gccaactcgc ttaacaccaa aatctttgcc agtacaacaa aaatcaatgc
     6781 cagaccgccg tgggtaccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     6841 acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga atcccccggt
     6901 catcagcacc agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg
     6961 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt
     7021 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     7081 cagacgcgta ataaatgaga gcatcgccca aaataattta ctcatttgat ttttactcca
     7141 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc
     7201 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     7261 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     7321 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     7381 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattcaag caacacaacc
     7441 tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     7501 cgccagtgct ccgggcggcc ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     7561 aggatttgcg aggtagcgat atacagaacc tgtgaagagt ccccaataag cgcctctgcg
     7621 tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa tcatcatacc gactcctgat
     7681 tcaaatgtat gatggtattc attgaacaga acaatggatc ccccaccgtt gctgaccgca
     7741 aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca
     7801 ttacgaaata agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa
     7861 tgcgtcattg acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc
     7921 tactgccgga acaa
//
LOCUS       DAZFKY010000198         7794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFKY010000198 DAZFKY010000000
VERSION     DAZFKY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(197..340)
                     /gene="ypaB"
                     /locus_tag="ACHKCL_004058"
     CDS             complement(197..340)
                     /gene="ypaB"
                     /locus_tag="ACHKCL_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5764923.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            468..2753
                     /gene="nrdA"
                     /locus_tag="ACHKCL_004059"
     CDS             468..2753
                     /gene="nrdA"
                     /locus_tag="ACHKCL_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5764924.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2980..4110
                     /gene="nrdB"
                     /locus_tag="ACHKCL_004060"
     CDS             2980..4110
                     /gene="nrdB"
                     /locus_tag="ACHKCL_004060"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5764925.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4110..4364
                     /gene="yfaE"
                     /locus_tag="ACHKCL_004061"
     CDS             4110..4364
                     /gene="yfaE"
                     /locus_tag="ACHKCL_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5764926.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4418..5068)
                     /gene="inaA"
                     /locus_tag="ACHKCL_004062"
     CDS             complement(4418..5068)
                     /gene="inaA"
                     /locus_tag="ACHKCL_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5764927.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5149..6339)
                     /locus_tag="ACHKCL_004063"
     CDS             complement(5149..6339)
                     /locus_tag="ACHKCL_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5764928.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6491..7369
                     /locus_tag="ACHKCL_004064"
     CDS             6491..7369
                     /locus_tag="ACHKCL_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5764929.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2096 a         2010 c         1832 g         1856 t
ORIGIN      
        1 aaaatggctt cgcatttaac aataaccgaa tagaaaacaa ccatttcgac atcaacaatc
       61 gcttacattc gcttatatat tgaccacaac tgatacatca gattatgtga tgactcgtgc
      121 ttagatcaat ttttgcaatc attagcaaaa agattaataa accatctata tcaatttatc
      181 caacctattc tgccgttcaa gaaatcgccg aacagttatt tataacaaat ttttctcttc
      241 ccattgactt tcccggacac cttgtctgac ctaaggtgcg cgaaagccac tttttccttc
      301 ctgagttatc cacaaagtta tgcacttgca agagggccat tttcacacta tcttgcagtg
      361 aatcccaaac atacccccta tatatagtgt tctaagcagc ttcccgtact acaggtagtc
      421 tgcacgaaac tattgcggaa agaattccaa aaacaggtac gacatacatg aatcagaatc
      481 tgctggtgac aaagcgcgac ggtagcacag agcgcatcaa tctcgacaaa atccatcgcg
      541 ttctggattg ggcggcagaa ggactgcata acgtttcgat ttcccaggtc gagctgcgct
      601 cccacattca gttttatgac ggtatcaaga cctctgacat ccacgaaacc attatcaagg
      661 ctgccgcaga cctgatctcc cgtgatgcgc cggattatca gtatctcgcc gcgcgcctgg
      721 cgatcttcca cctgcgtaaa aaagcctacg gccagtttga gccgcctgcg ctgtacgacc
      781 acgtggtgaa aatggtcgag atgggcaaat acgataatca tctgctggaa gactacacgg
      841 aagaagagtt caagcagatg gacacgttta tcgatcacga ccgtgatatg accttctctt
      901 atgctgccgt taagcaactg gaaggcaaat atctggtaca gaaccgtgtg accggcgaaa
      961 tctacgaaag cgcccagttc ctttatattc tggttgctgc gtgcctgttc tcgaactacc
     1021 cgcgtgaaac gcgcctgcaa tatgtgaagc gtttttacga cgcggtttcc acatttaaaa
     1081 tttcgctgcc gacgccaatc atgtccggcg tgcgtacccc gactcgtcag ttcagctcct
     1141 gcgtactgat cgagtgcggc gacagcctgg attccatcaa cgccacctcc agcgcgattg
     1201 tgaaatatgt ttcccagcgt gccgggatcg gcatcaacgc cgggcgtatt cgtgcgctgg
     1261 gtagcccgat tcgcggtggc gaagcgttcc ataccggctg cattccgttc tacaaacatt
     1321 tccagactgc ggtgaaatcc tgctctcagg gcggcgtgcg cggcggtgcg gcaacgctgt
     1381 tctacccgat gtggcatctg gaagtggaaa gcctgctggt gttgaaaaac aaccgtggtg
     1441 tggaaggcaa ccgcgtgcgt catatggact acggggtaca aatcaacaaa ctgatgtata
     1501 cccgcctgct gaaaggtgaa gatatcaccc tgttcagccc gtccgacgta ccggggctgt
     1561 acgacgcatt cttcgccgat caggaagagt ttgaacgcct gtataccaaa tatgagaaag
     1621 acgacagcat ccgcaagcag cgtgtgaaag ccgttgagct gttctcgctg atgatgcagg
     1681 aacgtgcgtc taccggtcgt atctatattc agaacgttga ccactgcaat acccatagcc
     1741 cgtttgatcc ggccatcgcg ccagtgcgtc agtctaacct gtgcctggag atagccctgc
     1801 cgaccaaacc gctgaacgac gtcaacgacg agaacggtga aatcgcgctg tgtacgctgt
     1861 ctgctttcaa cctgggcgca attaatagcc tggatgaact ggaagagctg gcaattctgg
     1921 cggttcgtgc acttgacgcg ctgctggatt atcaggatta cccgatcccg gccgccaaac
     1981 gtggagcgat gggtcgtcgt acgctgggta ttggtgtgat caacttcgct tactacctgg
     2041 cgaagcatgg taaacgctac tccgacggca gcgccaacaa cctgacgcat aaaaccttcg
     2101 aagcgattca gtattacctg ctgaaagcgt ctaatgagct ggcgaaagag caaggcgcgt
     2161 gcccgtggtt taacgaaacc acttacgcga aagggatcct gccgatcgat acctataaga
     2221 aagatctgga tgccatcgct aatgaaccgc tgcattacga ctgggaagct ctgcgtgagt
     2281 caatcaaaac acacggcctg cgtaactcca cgctttctgc tctgatgccg tccgagactt
     2341 cttcgcagat ctctaacgcc actaacggta ttgaaccgcc gcgcggttac gtcagcatca
     2401 aagcgtcgaa agacggtatt ctgcgccagg tggtgccgga ctacgagcat ctgcacgacg
     2461 cctatgagct gctgtgggaa atgccgggta acgatggcta cctgcaactg gtgggtatca
     2521 tgcagaaatt tatcgatcag tcgatctctg ctaacaccaa ctacgatccg tcacgcttcc
     2581 cgtcaggaaa agtgccgatg cagcagttgc tgaaagacct gctcaccgcc tacaaattcg
     2641 gggtcaaaac gctgtattat cagaacaccc gtgacggcgc tgaagacgcg caggacgatc
     2701 tggtgccgtc aatccaggac gatggctgcg aaagcggcgc atgtaagatc tgatattgag
     2761 atgccggatg cggcgtaaac gccttatccg gcctacggct cggtttgtag gcctgataag
     2821 acgcgccagc gtcgcatcag gcacaggatg cggcgtgaac gcctaatccg gcctacggct
     2881 cggatttgta ggcctgataa gacgcgccag cgtcgcatca ggcacaggat gcggcgtaaa
     2941 atgccttatc cggcattaaa ctcccaacag gacacactca tggcatatac caccttttca
     3001 cagacgaaaa atgatcagct caaagaaccg atgttctttg gtcagccggt caacgtggcc
     3061 cgttacgatc agcaaaaata tgacatcttc gaaaagctga tcgaaaagca gctctctttc
     3121 ttctggcgtc cggaagaagt tgacgtttct cgtgaccgta tcgattacca ggcgctgcct
     3181 gaacacgaaa aacacatctt tatcagcaac ctgaaatatc agacgctgct ggactccatt
     3241 cagggtcgta gcccgaacgt ggcactgctg ccgctgatct cgatcccgga actggaaacc
     3301 tgggtagaaa cctgggcgtt ctcagaaacg attcattccc gttcctatac tcatatcatt
     3361 cgtaatatcg ttaacgatcc gtctgttgtg tttgacgata tcgtcaccaa cgagcagatc
     3421 cagaaacgtg cggaagggat ctccagctac tacgatgagc tgatcgaaat gaccagctac
     3481 tggcacctgc tgggcgaagg cacccacact gttaacggta aaaccgtgac cgttagcctg
     3541 cgcgagctga agaaaaaact gtatctctgc ctgatgagcg ttaacgcgct ggaagcgatc
     3601 cgcttctacg tcagctttgc ttgttccttt gcctttgcag aacgcgaact gatggaaggt
     3661 aacgccaaaa ttattcgcct gattgcccgc gacgaagccc tgcacctgac cggtactcag
     3721 catatgctga atctgctgcg cagcggtgcg gatgatccgg agatggcgga aattgccgaa
     3781 gaatgtaagc aggagtgcta tgacctgttt gtccaggcgg cacagcaaga gaaagactgg
     3841 gcggattatc tgttccgtga cggttcgatg attggtctga ataaagacat tctctgccag
     3901 tacgttgaat acatcaccaa tatccgcatg caggcagtcg gtttggatct gccgttccag
     3961 acgcgctcca acccgatccc gtggatcaac acctggctgg tgtctgataa cgtgcaggtt
     4021 gctccgcagg aagtggaagt cagttcttat ctggtcgggc agattgactc ggaagtggac
     4081 accgacgatt tgagtaactt ccagctctga tggctcgcgt taccctgcgc atcactggca
     4141 cacaactgct gtgccaggat gaacaccctt cccttctggc ggcgctggaa tcccacaatg
     4201 tggcggttga gtaccagtgc cgcgaaggtt actgcggctc ctgtcgcaca cgcctggttg
     4261 caggccaggt ggactggatt gccgaaccgt tagcctttat tcagccgggg gaaattttgc
     4321 cctgttgttg ccgagcaaaa ggcgatattg aaatcgagtt gtgatcgggt actagcgcca
     4381 gatacgacgc ttacgcgtat tatctggcct acaacgatta catggcgtag taatacgctt
     4441 ttacctgctc ccagtccgct ttggggatcg gctccagata tttttccagc tggcggaagt
     4501 catgattaat cgctttatcg cgacgcaagc ggcgacgact tttctccaga tcaagaaaac
     4561 cagcttctgc cttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     4621 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     4681 gtacttcgtc agaatagggc gttactgcat gctgggcata ccagtcagca atgctgatga
     4741 accccgccat atcttcagtc accagcaacg cgcgccattc cccctcaatt ttcaccgctt
     4801 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     4861 cctcacggac aatcgttggt cggccgaaag gataacgtac ggaatgaaac agatgatgcg
     4921 tcatgcgctt tacatatagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     4981 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     5041 attcgtcgtg ctttgctgaa actgccatac cctatcgcct gtcatttttt attaacgaca
     5101 atgactatag gtggttacct gcgaaaaatc ttaatgaaac gtgtcgtatt aatgaatttc
     5161 ttttgccact actagcgcga caccgctttt tcccttcatt tgcagcgcat agactccacc
     5221 agccactacc actaatagtg cgcccagcag ataaatcatt cggtagccag tatgtgcaac
     5281 catcatcccc atcacacagg ctcccagcgc caggcctgca tccatcgcat tgaagaagag
     5341 ggagtttgcg acaccgattt tatgcggttc cactgaactg ataatctgcg tctgaaaaac
     5401 tggcgtcacg gaaccatacc cgataccaat caacgcgcca gaaacgacca tcattacgct
     5461 gccctgggtg tagccgagaa ccaccagccc aaccgtaaag aacagcaggc aggggtaaac
     5521 cacatatttc gggccttttt tgtcgcaaac attgccggtg aaggtacggc agatcatcag
     5581 gaagatggcg tagcagagca ggaaattact ggccgccgac atcagattaa gttcgcgggc
     5641 gtaaagcgcc agaaaggcag aaaccccggc atacgaaaag gtcataaaga atgtaaccat
     5701 ggcaaacggc aatgccgcgc gatcgaacat ggcggcgaag cccatctttg gctttgaacc
     5761 gtcggcatgg ctgataaccg gcggcacggt cataatcagc gacaggataa tccccaccgc
     5821 cgcaacgcca gtacataacc agaaagccat attaaaggcc tcccagcgcg ccatattcag
     5881 gccaatccac ggcccgacca ccattgccag ccccattgcc agagagaaaa aactgatgcc
     5941 ctccccacgt cgggaggccg ggatcagccg cgcggaaatc gttcccttta ccgttgtgat
     6001 aaccccgaag gtaataccat gaagcaccct aataaaaagc agtgattcta ttgagtggca
     6061 aaaaggatac agcgcagtga ccaccaaaaa ggccagagaa gagagcacca gaatagtttt
     6121 attcgaatat ttacccaccc attgcccggc aaagggtcga ataacaatcg ccgcaattaa
     6181 aaataaagtc accaacaaac ctgctttatc aggagaggca tgcaagcggt cagagatata
     6241 aattggcaac aatgttagta aaacataaaa gacgaagaaa ataataaagc tgatgatggt
     6301 tattgcccaa aaatccttcg tccataactt ctctttcata ctctaccgcc tgtcaggatg
     6361 acgcacgcag acaacactcc cgttactgcg gttgcgtcaa attgtgatgt actttgcaga
     6421 acagaagtat aaatggcggc atgctattag agaaattaat cgttttaata ttattgatta
     6481 gcaaactcta atgactctta cccaaataaa ttctttactc gccgtgctgg attacggtgg
     6541 ctttactgaa gccagcaagc gattgttcat gacgcagtcg gcggtaagtc aggcaattgc
     6601 tgccctcgaa caagaacttg gtgtgaatat actgttacgc gatcgccgta aagctataca
     6661 actgaccaca gcaggtcacc gcattgtgca acatctgcgc gccattaatc gggaagttaa
     6721 cgcagtaaaa gagatagccg agcaggaaaa gcagaaccct caaagaacgc tgagacttgg
     6781 ttgtttcccc agcgtctgcg cctgtatctt accggttgtg attcgctact ttgaaattca
     6841 tcatccgaat ataaaaatta ttccttttga ggaaaatagc acagcgatta ttgattcgct
     6901 tcagaatgaa tccattgatg ccggttttgt tcattttccg gtgagcggaa tgtactccgt
     6961 gcccatttat caggataaat tcaccgtagt gttaccgccc aaccatgctc tggcaaaaaa
     7021 cagcactatt acagtggaag aattaatggg tgaaccatta atcattagta aaggacgcta
     7081 tgaattaagc attatggcac tatttaaaga aaagaatatt accccacaga taaaatatga
     7141 atttaatcac ccggatacag cgatcagttt tattcgacag ggattaggca tcgccttatt
     7201 accagagtta acgctgaaaa ccattgcaga tgaattgtgt tccgttcctc tcgaaccaac
     7261 cttctatcga cagatttcgt tgctggctaa agaaaagccg gtagaaggca gtccactgtt
     7321 tttactacaa atgtgtacgg aacaactagt ggtgagtgga aaaatttgat gtaagacatg
     7381 agattgatga caaatgcaag actgcctgat tcgccacact tagttgtact atagccttta
     7441 ccaggcgtaa taattcgttt tcacctgatt ttggcccact ttgtgaattg gcgatatatt
     7501 tatccagttg ggagaagtga caatgactcc aggtagaaac cggtatgaag cgcccgactc
     7561 actgtcaaaa ccgattgttg agagtaatga cactccggcg aacagtacac agtttactct
     7621 ggagccattc tcaatcttca agtgggatac ctaataactt gtcagggaag ttatcaatat
     7681 ctgtttgtta cttattaccg tatttattaa caataatgat actttctaac atgcgtaaag
     7741 gtcttcgtaa taaaatgagc taaatttgat agtgaatata ttctgcaaag atta
//
LOCUS       DAZFKY010000199         7674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFKY010000199 DAZFKY010000000
VERSION     DAZFKY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1736
                     /gene="adeD"
                     /locus_tag="ACHKCL_004065"
     CDS             3..1736
                     /gene="adeD"
                     /locus_tag="ACHKCL_004065"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5764930.1"
                     /translation="MSRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIK
                     GRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
     gene            complement(1782..3173)
                     /gene="uhpT"
                     /locus_tag="ACHKCL_004066"
     CDS             complement(1782..3173)
                     /gene="uhpT"
                     /locus_tag="ACHKCL_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5764931.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3311..4630)
                     /gene="uhpC"
                     /locus_tag="ACHKCL_004067"
     CDS             complement(3311..4630)
                     /gene="uhpC"
                     /locus_tag="ACHKCL_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5764932.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4640..6142)
                     /gene="uhpB"
                     /locus_tag="ACHKCL_004068"
     CDS             complement(4640..6142)
                     /gene="uhpB"
                     /locus_tag="ACHKCL_004068"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5764933.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6142..6732)
                     /gene="uhpA"
                     /locus_tag="ACHKCL_004069"
     CDS             complement(6142..6732)
                     /gene="uhpA"
                     /locus_tag="ACHKCL_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5764934.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6894..>7674)
                     /locus_tag="ACHKCL_004070"
     CDS             complement(6894..>7674)
                     /locus_tag="ACHKCL_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764935.1"
                     /translation="EKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKI
                     NSTISELLSKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDG
                     IKPEKIAKIISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSF
                     VALNGRNYHQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAED
                     DSPYVHSDESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
BASE COUNT         1974 a         2137 c         1937 g         1626 t
ORIGIN      
        1 acattagccg ggcggaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg
       61 attatattat tgataatgtc tctattctcg acctgattaa tggcggagaa atttccggcc
      121 caattgtgat taaagggcgt tacattgccg gtgttggcgc agaatacact gatgctccgg
      181 ctttgcagcg gattgatgcc cacggcgcaa cggcggtgcc agggtttatt gatgctcacc
      241 tgcatattga atccagcatg atgacaccgg tcacctttga aaccgccacc ctgcctcgcg
      301 gcctgacaac tgttatttgc gatcctcatg aaatcgtcaa cgtgatgggc gaagccggtt
      361 tcgcctggtt tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct
      421 cttgcgtacc cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga
      481 tgctcgcctg gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg
      541 gcgtaattag cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc
      601 tggacggtca ctgcccgggt ttgggtggta aagaacttaa cgcctatatt gctgcgggta
      661 ttgaaaactg ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca
      721 tgtcgttgat gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga
      781 tcaacgaatt taacagcccg caatgcatgc tctgtactga tgaccgtaac ccgtgggaga
      841 tcgcccatga aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc
      901 cgctgcatgt ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc
      961 acctcggctt actggcacct ggtaagcagg ctgatatcgt cctgttgagc gatgcgcgta
     1021 aggtcacggt gcagcaggta ctggtgaaag gcgaaccgat tgacgcgcaa accttacagg
     1081 cggaagagtc ggcgaaactg gcacaatccg ctccgccata tggcaacacc attgcccgcc
     1141 agccagtttc cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca
     1201 ttgacgtcat ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg
     1261 gttttgagcg caatgatgtg tgctttattg ccgtacttga gcgttacggg caacgtctgg
     1321 ctccggcttg tggtttgctc ggcggctttg gtctgaatga aggcgcgctg gctgcgacgg
     1381 tcagccatga cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctag
     1441 cggtcaatca ggtgattcag gatggcggcg ggttgtgcgt ggtacgtaac ggccaggtcc
     1501 aaagccatct gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg
     1561 cggagcaaat tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc
     1621 cgtttattca gatggcgttt ctttccctgc cagtgatccc cgcgctgaaa ctaaccagcc
     1681 aggggctgtt tgatggcgag aagtttgcct tcaccacgct ggaagtcact gaataataaa
     1741 aaaagcccgg cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc
     1801 tgaatttttt tctcgcggcg gattttgcgt tcttccatca ccgccactat cgccatcagg
     1861 cagatacaac caatcgcggc gatatccagc gcagcgaagg tgcctgccca gccggtaagg
     1921 ccgaatactg gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca
     1981 atcaggtaag cgaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca
     2041 aagccaacag cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca
     2101 aagagagaag ccagatagat atattcgttg ctggcatgtt ggtacacacc gagcgtggcg
     2161 ataatcagcg ccagcgcgat gcaggccacc aggccacggc gaccgttcgc taggtcagag
     2221 agccagcccc acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga
     2281 atcgccaccg ctttagagag tttcagttcc tggaacgcgt acacagttga ccactggtcg
     2341 ataccaatac gcaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg
     2401 tttttcagca catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct
     2461 ttgtcctctt cgctgatctc ctcgccgaac agctcttcag ctttgccgag gccataagat
     2521 tccggggagt cgctgccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac
     2581 gggaagataa acatgccgat gacatggcca tcgaacaggt aatttgcgcc gaacagtgcc
     2641 acaccagctg cgcctgcacc gccaaggttg tgagaaatgt tccagaaacc gaggaacgtc
     2701 ccgcgtttac gacgcggcgt ccatttggtg atggtggagt aactgcacga acccccggta
     2761 ctctggaaaa agccacttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg
     2821 ctgcccatac tggcgctaaa gcccagcata caaatggcag acaggatcag catgaacggc
     2881 aggaattgtt tggtgttttt gccgtcggcg tagtaggaaa ccagcgtttt acccacgcca
     2941 taagtgatgg agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc
     3001 gaaatcatat cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag
     3061 ccgataaaga ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact
     3121 tcgagcggaa ggtccagggt cggcttgcgg acctggttta agaaagccag catgggttac
     3181 tcctgaaatg aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg
     3241 agatagcgtc caggcaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag
     3301 gtgagatgca tcacgcttcg cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg
     3361 cggaaatccc ggcggcaatg gcgataacca caaaaaatcc gctccagtgc caggtatcga
     3421 gcactttcgc cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa
     3481 accccgtcgc cgcccctgcc gcctctttgt gggaacattc tgccgccgcc atgccgatta
     3541 acatctgcgg gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt
     3601 agctggcaaa tggcatcagc cacagcgagc cgactgaaag caaaattccg gcagcgaaaa
     3661 tcaaattcat cggcccgcgg ttgccgttaa acagtttgtc cgagccccaa cccgctacca
     3721 gcgcaccgat aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat
     3781 cgacgcccag cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccgaacca
     3841 catagaccag cacatagcaa aacgaaagca gccagatata cggattcaac aacacatact
     3901 tggtgaggat ctctttacgc gtcagccctg ccccttcttg ttgttgagca atctccagcg
     3961 cgtcgtgtcg ccattcaccg accgctggta aacctaacgc ctgcgggcga tcgcgcagcc
     4021 gccagcagag aaaaatcccc acgacgatcg ccatacaacc agcaatcatc atcccggcac
     4081 gccagccgta atgcagcgca gctgctgcca tcacaatggg aatgagcgca ccgccaacgt
     4141 tatgcgccgt gttccataat gcccaccaac caccgcgttc ggtacgagaa taccaggccg
     4201 ttaacagacg cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga
     4261 gcacggcaaa cgcccatagc gacgtcgaga agccaaacag aatgttgatg atgcccgtgg
     4321 cgataagccc tatccccata aaataacggg catttgagcg gtcgctgaca atgccggaga
     4381 caaacttcga cacgccgtag gtaatgtaaa acagggtcgc taacaggccg atatcgctac
     4441 ggctgagcac gccattagca aggatttctg gtacggcggc gttaaaactt ttacgcgtga
     4501 agtaaaacag cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat
     4561 aacgggcatc aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa
     4621 acggcaacat catcaaacct tagacatagc gttgaggtag agaaacgctg acacgcgtgc
     4681 cgtgcagaca ggaaatggtc aaggtgccgc ccagcgccgt tacgcgctcg cgcattccgg
     4741 tgaggccaaa accttgttgc ccggaacccg gcggtaaacc gctgccatcg tcttcaatca
     4801 ccagcatcaa ccgttcatcc tgcagccagc cttgcagggt gaccgcgctg gcatcagcat
     4861 gtttcacaat gttattcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt
     4921 cgcttaacgc tgactcttcg attcgccatt cgagatggct gacaatgccg cgcccttcca
     4981 gctccatttc ccgcatcagt gagcggatgg cctgctctag ggtgagatca tccaactggc
     5041 gcggacgtaa ccgccctaac aaacggcgca ccgcgtcgta cacgcccagc gacagttgtt
     5101 cgatgagctg cccgctctgc ttcacactgg cgttatctgc cgccagtcgc tggacaatgc
     5161 ccgcttgagt acgaatggca gtgatggtct ggccgatatc atcatgcagc tcacgcgcca
     5221 catcacggcg cacgctctct tcagtttcta acaaacgttc agcgagatgc tgattgcgcg
     5281 ccagttcctt ttgcagcgac tggttaagtt cacgcaaccg ctggatgcca gcgcccagta
     5341 acaaccctgt cagactttgc accagcagcg agagcaataa atccacagga tgatcgcgcc
     5401 aggtttgact ggcgatcagc gcgatggcgt tcatcaatgt cgctatcagc gccccttgcc
     5461 agccgtagtg ccaggccagc gcgataatcg gcagcgccag gcagaatggc gtaaagcgcg
     5521 acagttcgtc cggcaagccc aactggagcc agagactgat aacaaacagc agcaagtacc
     5581 agaccagatg ccgcccgcgc cagttgatcg gttgagaaac taacgacggc ccgagcggca
     5641 gccaggtgtt attggcgaga taatgccaga acaccagaca tatcggggcc agcgtcaggc
     5701 cgccagttaa agttaacagc agcgcattcc acgactcttt gccgtgccaa agccagggca
     5761 gcgactgcaa caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt
     5821 cacgttgatg gcgatagcgc gagatcagcg ccacgggcag taacgtcagt aaactaccga
     5881 tcatcaataa ggagaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc
     5941 actccgctcc cagcagcacc ggccagtatc cgcgcgggca ttgcagcatt agccctagcc
     6001 gcagaccaaa cggaaataac agcaccgcca tatcagggcg ctcaaccagg tgcaggctga
     6061 tactccacag gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta
     6121 agcgggagaa caacgtcttc atcaccagcc atcaaacata cggcgcgcca gttcaacgtc
     6181 gttactgacg cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga
     6241 caagcccagt tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac
     6301 ctgccgttca cgtttggtta gtgggtcctg gcgaccggat gccagtttaa tggcaatatc
     6361 cggcgtcaga taacagccgc ctgtggcaac cgtatgcacc gcagcaatca gttcgtcagg
     6421 gctacaacgc ttggagagaa agccacgcgc ccccgcgtta agcgcctgct caaccagcgc
     6481 cggactgtca tgaacggaga gcataattgt cgccatacct ttcggcagct ggcttagcag
     6541 ctccagaccg gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc
     6601 cggcagcccc gccagcgcct cgcgccccga accaaactcg gcaactactt gcaaatcagg
     6661 ttccagcccc agcaactgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc
     6721 aacggtgatc atgattgggt cctggcggat aaaaaacgcg cataccttaa cgataagcgc
     6781 gatgttgttc aagttggata tggggtatgt attggcttaa tgatgttgag cttatcgtca
     6841 aaacgcttac ctgagaggta agcgtttggt gcgtgttatt gagaaaaagc gaattatttg
     6901 taggttgcaa tcgtaatatt gcctgagaaa tttctcctta gattgcggat atcattatcc
     6961 cttgcccaat aatcatcttt gtgtctataa ctctcatcgg aatgtacata gggagaatca
     7021 tcttcagcgt cgttatggat aatattttcg cctttagtgg tatagatata attagcaaca
     7081 tcaaaggtgt cattcgcgtt atttacgact gcgcctaaga cgcaaagggt ttgagtgaat
     7141 ccgtattctg agaaatcgac atgctgatgg taatttctac cattcaaagc aacaaaactc
     7201 ggacaaacat ctattgtatc gccaacagac gtagcggcgc gacgcacatc aagtgcagcg
     7261 ttatcgttag caacatgaaa tgcttgtgaa caggcataaa acaaatttct aatcccggta
     7321 cgactggata aacaagaaat aatttttgct attttttctg gctttatacc atcctgtact
     7381 aaagaatctc tcaccagatc acattcacca ggtgtgaaaa caactttctt gaatatttcc
     7441 tcataaacat cataagaaaa atattcagag agagtgaaag attcggtggg atgtgttaaa
     7501 tctgtaattt ttttgctaag taattcactt atagtgctgt ttattttttc accgcaacat
     7561 aaaacaaatg aacttccctc cagaacttgc ctttctgcat ctacaaggag tgagctaaat
     7621 tcatcctcat gaagcttaag tttttttata cctgattgat gtaaatattt ttct
//
LOCUS       DAZFKY010000200         7661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFKY010000200 DAZFKY010000000
VERSION     DAZFKY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..205
                     /locus_tag="ACHKCL_004071"
     CDS             <1..205
                     /locus_tag="ACHKCL_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764936.1"
                     /translation="IRNHASEFTDLIILILIYFEKGIGIYAVFLKILIIGMDFRARKG
                     FYMDTLLIISSSHHQQFTSLFRV"
     gene            298..2721
                     /gene="pgaA"
                     /locus_tag="ACHKCL_004072"
     CDS             298..2721
                     /gene="pgaA"
                     /locus_tag="ACHKCL_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO5764937.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2730..4748
                     /gene="pgaB"
                     /locus_tag="ACHKCL_004073"
     CDS             2730..4748
                     /gene="pgaB"
                     /locus_tag="ACHKCL_004073"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO5764938.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4741..6066
                     /gene="pgaC"
                     /locus_tag="ACHKCL_004074"
     CDS             4741..6066
                     /gene="pgaC"
                     /locus_tag="ACHKCL_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO5764939.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6068..6481
                     /gene="pgaD"
                     /locus_tag="ACHKCL_004075"
     CDS             6068..6481
                     /gene="pgaD"
                     /locus_tag="ACHKCL_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO5764940.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6531..7319)
                     /gene="phoH"
                     /locus_tag="ACHKCL_004076"
     CDS             complement(6531..7319)
                     /gene="phoH"
                     /locus_tag="ACHKCL_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5764941.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT         2078 a         1767 c         1795 g         2021 t
ORIGIN      
        1 aatccgaaat catgcatcgg aatttactga tttaattatt ttaatcctaa tttattttga
       61 aaaaggcatc gggatttatg ccgtattcct gaagatcctc atcattggaa tggattttcg
      121 ggcgagaaaa ggattttata tggacactct gctcatcatt tcttcttctc atcatcaaca
      181 attcacgtct ctcttccgcg tttaataacg gattatgagg tgcaaaaata tctttctttt
      241 cagttacctg taattagata cagagagaga ttttggcaat acatggagta atacaggatg
      301 tattcaagta gcagaaaaag gtgcccgaaa accaaatggg ctttgaaact tcttactgcc
      361 gcatttttag cagcgagtcc cgcggcgaag agtgctgtta ataacgccta tgatgcattg
      421 attattgaag ctcgcaaggg taatactcag ccagctttgt catggtttgc actaaaatca
      481 gcactcagca ataaccaaat tgctgactgg ttacagattg ccttatgggc cgggcaagat
      541 aaacaggtta ttaccgttta caaccgctac cgtcatcagc aattaccagc gcgtggttat
      601 gcagctgtcg ccgtcgctta tcgtaacctg caacaatggc aaaactcgct tacactgtgg
      661 caaaaggcgc tctctctgga gccgcaaaat aaggattatc aacggggaca aattttaacc
      721 ctggcagatg ctggtcacta tgatactgcg ctggttaaac ttaagcagct taactctgga
      781 gcaccggaca aagccaattt actcgcagaa gcctatatct ataaactggc ggggcgtcat
      841 caggatgaat tacgggcgat gacagagtca ttacctgaaa atgcatctac gcaacaatat
      901 cccacagaat acgtgcaggc attacgtaat aatcaacttg ctgccgcgat tgacgatgcc
      961 aatttaacgc cagatattcg cgctgatatt catgccgaac tggtcagact gtcgtttatg
     1021 cctacgcgca gtgaaagtga acgttatgcc attgccgatc gcgccctcgc ccaatacgct
     1081 gcattagaaa ttctgtggca cgataaccca gaccgtactg cccagtacca gcgtattcag
     1141 gttgatcatc ttggcgcgtt attaactcgc gatcgttata aagacgttat ttctcactat
     1201 cagcgattaa aaaagacggg gcaaattatt ccgccctggg ggcaatattg ggttgcatcg
     1261 gcttatctca aagatcatca gccgaaaaaa gcacagtcaa taatgaccga gctcttttat
     1321 cacaaggaga ccattgcccc ggatttatcc gatgaagaac ttgcggatct cttttacagc
     1381 cacctggaga gtgaaaatta tccgggcgcg ctaactgtca cccaacatac cattaatact
     1441 tcgccgcctt tccttcggtt aatgggcacg cctacgagca tcccgaatga tacctggtta
     1501 caggggcatt cgtttctctc aaccgtagca aaatatagta atgatcttcc tcaggctgaa
     1561 atgacagcca gagagcttgc ttataacgca ccaggaaatc agggactgcg cattgattac
     1621 gcgagtgtgt tacaagcccg cggttggcct cgtgcagcag aaaatgaatt aaaaaaagca
     1681 gaagtgatcg agccacgtaa tattaatctg gaggttgaac aagcctggac agcattaacg
     1741 ttacaagaat ggcagcaggc agctgtctta acgcacgatg ttgtcgaacg tgaaccgcaa
     1801 gatcccggcg ttgtacgatt aaaacgtgcg gttgatgtac ataatcttgc agagcttcgt
     1861 atcgctggct caacaggaat tgatgccgaa ggcccggata gtggtaaaca tgatgtcgac
     1921 ttaaccacca tcgtttattc accaccgctg aaggataact ggcgcggttt tgctggattc
     1981 ggttatgccg atggacaatt tagcgaagga aaagggattg ttcgcgactg gcttgcgggt
     2041 gttgagtggc ggtcacgtaa tatctggctc gaggcagagt acgctgaacg cgttttcaat
     2101 catgagcata aacccggcgc gcgcctgtct ggctggtatg attttaatga taactggcgt
     2161 attggttcgc aactggaacg cctctctcac cgcgttccat tacgggcaat gaaaaatggt
     2221 gttacaggca acagtgctca ggcttatgtt cgctggtatc aaaatgagcg gcgtaagtac
     2281 ggtgtctcct gggctttcac tgatttttcc gacagtaacc agcgtcatga agtctcactt
     2341 gagggtcagg aacgcatctg gtcttcacca tatttgattg tcgatttcct acccagtctg
     2401 tattacgaac aaaatacaga acacgatacc ccatactaca accctataaa aacgttcgat
     2461 attgttccgg catttgaggc aagccatttg ttatggcgaa gctatgaaaa tagctgggag
     2521 caaatattca gcgcaggtgt tggtgcctcc tggcaaaaac attatggcac ggatgtcgtc
     2581 acccaactcg gctacgggca acgcattagc tggaatgacg tgattgatgc tggcgcaacg
     2641 ctacgctggg aaaaacgacc ttatgacggt gacagagaac acaacttata cgttgaattc
     2701 gatatgacat tcagatttta aggataaata tgttacgtaa tggaaataaa tatctcctga
     2761 tgctggtgag tataattatg ctcaccgcgt gcattagcca gtcaagaaca tcatttatac
     2821 cgccacagga tcgcaaatct ttactcgccg agcaaccgtg gccgcataat ggttttgtag
     2881 cgatttcatg gcataacgtt gaagacgaag ctgccgacca gcgttttatg tcagtgcgga
     2941 catcagcact gcgtgaacaa tttgcctggc tgcgcgagaa cggttatcaa ccggtcagta
     3001 ttgctcaaat tcgtgaagca catcgaggag gaaaaccgct accggaaaaa gctgtagtgc
     3061 tgacttttga tgacggctac cagagttttt atacccgcgt cttcccaatt cttcaggcct
     3121 tccagtggcc tgctgtatgg gcccccgtcg gcagttgggt cgatacgcca gcggataaac
     3181 aagtaaaatt tggcgatgag ttggtcgatc gagaatattt tgccacgtgg caacaagtgc
     3241 gagaagttgc gcgttcccgg ctcgttgagc tcgcttctca tacatggaat tctcactacg
     3301 gtattcaggc taatgccacc ggcagcttat tgcctgtata tgtaaatcgt gcatatttta
     3361 ctgaccacgc acggtatgaa accgcagcag aataccggga aagaattcgt ctggatgctg
     3421 taaaaatgac ggaatacctg cgtacaaagg ttgaggtaaa tccacacgtt tttatttggc
     3481 cttatggcga agcgaatggc atagcgatag aggaattaaa aaaactcggt tatgacatgt
     3541 tcttcaccct tgaatcaggt ttggcaaatg cgtcgcaatt ggattccatt ccgcgggtat
     3601 taatcgccaa taatccctca ttaaaagagt ttgcccagca aattattacc gtacaggaaa
     3661 aatcaccaca acggataatg catatcgatc ttgattacgt ttatgacgaa aacctccagc
     3721 aaatggatcg caatattgat gtgctaattc agcgggtgaa agatatgcaa atatcaaccg
     3781 tgtatttgca ggcatttgct gatcccgatg gtgatgggct ggtcaaagag gtctggtttc
     3841 caaatcgttt gctaccaatg aaagcagata tttttagtcg ggttgcctgg caattacgta
     3901 cccgctcagg tgtaaacatc tatgcgtgga tgccggtatt aagctgggat ttagatccca
     3961 cattaacgcg agtaaaatac ttaccaacag gggagaaaaa agcacaaatt catcctgaac
     4021 aatatcaccg tctctctcct ttcgatgaca gagtcagagc acaagttggc atgttatatg
     4081 aagatcttgc cggacatgct gcttttgatg gcatattgtt ccacgatgat gctttgcttt
     4141 cagattatga agatgccagt gcaccggcta tcacggctta tcagcaagca ggctttagcg
     4201 ggagtctgag cgaaattcga caaaacccgg agcaatttaa acagtgggcc cgctttaaaa
     4261 gtcgtgcgtt aactgacttc actttagaac ttagtgcgcg cgtaaaagcc attcgcggtc
     4321 cacatattaa aactgcacga aatatttttg cacttccggt aatacaacct gaaagtgaag
     4381 cctggtttgc acagaattat gctgatttcc taaaaagcta tgactggacc gctattatgg
     4441 ctatgcctta tctggaaggt gtcgcagaaa aatcggctga ccaatggtta atacaattga
     4501 ccaatcaaat taaaaacatc cctcaggcta aagacaaatc tattttagaa ttacaggcac
     4561 aaaactggca gaaaaatggt cagcatcagg ctatttcttc gcaacaactc gctcactgga
     4621 tgagcctatt acaactgaat ggagtgaaaa actatggtta ttatcccgac aattttctgc
     4681 ataaccaacc tgaaatagac cttattcgtc ctgagttttc aacagcctgg tatccgaaaa
     4741 atgattaatc gcatcgtatc gttttttata ttatgtctgg tgttatgcat acccctatgc
     4801 gtagcgtact ttcactctgg tgaactgatg atgaggttcg ttttcttctg gccgtttttt
     4861 atgtccatta tgtggattgt tggcggcgtc tatttctggg tctatcgtga acgccgctgg
     4921 ccgtggggag aaaacgcacc agctccccag ttgaaagata atccgtctat ctccattatc
     4981 attccctgtt ttaatgagga gaaaaacgtt gaggaaacca tacacgccgc tttagcacag
     5041 cgttatgaga acattgaagt tattgccgta aatgacggtt caacagataa aacccgtgcc
     5101 atcctggatc gcatggctgc acaaattccc catttgcggg tcattcatct ggcgcaaaac
     5161 caggggaaag ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga atatctggtg
     5221 tgcattgatg gcgatgcgtt attagaccgc gatgcggcgg catatattgt ggaaccgatg
     5281 ttgtacaacc cgcgtgtggg tgccgtaacc ggtaatcctc gtattcgaac acgttctacc
     5341 ctggtgggta aaattcaggt tggcgagtat tcctcaatta ttggtttgat caagcgaacc
     5401 cagcgtatct atggaaacgt atttaccgtt tccggtgtta ttgccgcatt tcgtcgcagc
     5461 gccctggcag aagtgggtta ctggagtgac gatatgatca ccgaagatat tgatattagc
     5521 tggaagctgc agttgaatca gtggacgatt ttttacgagc cacgggcact gtgctggata
     5581 ttaatgcctg aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt
     5641 gcagaagtat tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg
     5701 ccgctgtttt ttgaatacag cctgacgaca atatgggcct tcacctgcct ggtcggtttc
     5761 attatttacg cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct
     5821 gcgacacata ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc
     5881 ctgatgatcg agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg
     5941 ttcccggtta ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc
     6001 atgttgatgc ctaaaaagca acgcgcccgt tgggtaagtc ccgatcgcgg gattctgaga
     6061 ggttaatatg aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga
     6121 ttatgttgcc acaaccatct tgtggacatt atttgcgttg ttcatattct tattcgccat
     6181 ggatctgctg acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt
     6241 tttgctggca gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg
     6301 ttttcaaaaa cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag
     6361 cttagcaata cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca
     6421 cttcaccagc caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tcagggcata
     6481 aacacccaaa acaaagcccg gttagcccgg gctctgcacc gataatacac ttaactgtat
     6541 gcatgcagcg tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg
     6601 atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg
     6661 ccgcgaggca aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg
     6721 aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg
     6781 acgactgcat tttcaaaggt acgtccacgc atataggcga acggcgcaat ttccaccttc
     6841 cctatttccg gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcacg
     6901 tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga tatctccagg taagaagcca
     6961 agatcttcat cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt
     7021 atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc
     7081 gtggcgaata tcagctgctt actctcaata gccttcagat agtgcaattg cgcttcattt
     7141 cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca taccaatggc ttctacgccg
     7201 cccatctgca caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc
     7261 cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg
     7321 gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcgca
     7381 cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa
     7441 aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg aaaagaaagg atgaggttga
     7501 aaatgcaaag tgacgagatg actaccggag gagaaaactc cgcgagtggt ggcgcgttga
     7561 ttatctaaaa catgtccagt acaggacgtt accatccgcg atctccatag tgactgacta
     7621 tcactgccgg gaacttccgc tgctacttaa taagtacaac a
//
LOCUS       DAZFKY010000201         7526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFKY010000201 DAZFKY010000000
VERSION     DAZFKY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            7..1029
                     /locus_tag="ACHKCL_004077"
     CDS             7..1029
                     /locus_tag="ACHKCL_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5764942.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
     gene            1029..2009
                     /locus_tag="ACHKCL_004078"
     CDS             1029..2009
                     /locus_tag="ACHKCL_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5764943.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2006..2764
                     /locus_tag="ACHKCL_004079"
     CDS             2006..2764
                     /locus_tag="ACHKCL_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5764944.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2774..3586
                     /locus_tag="ACHKCL_004080"
                     /pseudo
     CDS             2774..3586
                     /locus_tag="ACHKCL_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            3583..4437
                     /gene="modD"
                     /locus_tag="ACHKCL_004081"
     CDS             3583..4437
                     /gene="modD"
                     /locus_tag="ACHKCL_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5764945.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4463..6433
                     /locus_tag="ACHKCL_004082"
     CDS             4463..6433
                     /locus_tag="ACHKCL_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5764946.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     NDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(6483..6737)
                     /locus_tag="ACHKCL_004083"
     CDS             complement(6483..6737)
                     /locus_tag="ACHKCL_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5764947.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6938..>7526
                     /locus_tag="ACHKCL_004084"
     CDS             6938..>7526
                     /locus_tag="ACHKCL_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5764948.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
BASE COUNT         1825 a         1997 c         1922 g         1782 t
ORIGIN      
        1 taacttatgt cactaaccag acggaggttt acacagattc ttgcgtcgac gttgttcctg
       61 catcatctgc cttcttttgc acaatcagtc aaattctggg cctcactgac gcttcccgaa
      121 gcccaaaaca ttacacgcat cgtcagcgca ggtgcgcccg ccgatttgct attgctggct
      181 gtcgcgccag aaaaaatggt cggtttttcc tcttttgatt ttgcccgtca ggcattaatt
      241 ccattgtcag agcacattcg ccagttaccc aggctgggac gactcgccgg gcgcgccagc
      301 acactctcgc tggaagggct tatggcgtta catcccgatt tggtcgttga ttgcggcaat
      361 acggatgaaa cctggatctc ccaggcacgg caggttagcg aacagacaca aataccctgg
      421 ttattgctta acgggaaact ggcgcaatca gcagaacagt taacaacgct tggcaaaacg
      481 ttaggcgaag agcatcgcgc cgcagaacaa gccaatctcg ccagccgctt cgttggtgaa
      541 gctcaggcat tcgccgcctc acccgccgct aacctccgct tttatgctgc gcgcggccct
      601 cgagggctgg agacgggctt aaagggttcg ttgcataccg aggcggcgga attattaggt
      661 ttgcacaacg tcgcgcaaat agccgatcgc caaggcctga cacaggtttc catggaaaat
      721 ctcctgcgct ggcagccgga tattattctg gttcaggagg ccgttactgc agattttatt
      781 cgtcgtgatc ctctctggca tggcgtgaaa gccgttgcag aacaacgcat cttattttta
      841 agtggcttgc cctttggctg gctggatgcc ccaccgggaa tcaaccgtct tctgggatta
      901 cgcagacttc acgcctggct ggatcccgcc atcaatcgcc agtttaaaag tgacatgcag
      961 cattacgccc aactgttctg gcattgttca ctcagtgacg ccgactatca aaaattggtg
     1021 gcgacctaat gagaatcgta aacggatgta ttttgctgct ggcagctatc agtatcactt
     1081 ttgccgccgt aagtggggct tatcatctcg atatgcagca actgctggcg ctaattcttc
     1141 gtcaagaaaa tgttcctgtg caggaacaaa ttgtcttctg gcaaatccgc gtgccgcgta
     1201 ttcttgctgc gctgttactg ggcgcggcgc tggcaggagc cggaaccacc tatcagggaa
     1261 tgttgcgtaa tcccttagtt tcaccggata ttttaggcgt ctccgccggt gccggacttg
     1321 gtgcctgcac ggcaattctg tggggattat ccattgttta catccagctg tacgcatttt
     1381 gtggcgggct gatggtggtc gcgggcgtct ggttgattac gcgtcgggtg acccgccacg
     1441 atccgatctt aaccctggtg ctggtaggca tcgcgttagg aacgttgtgc ggagcgggga
     1501 tatcgctaat taaaacgctg gccgatcctt acacccagtt accgtcaatt accttctggt
     1561 tgctgggggg actgtccacc gtgaccttac gcgatctctg ctatgctgca ccgatcattc
     1621 tcataggctc cttgcccctg ttttttctcc gctggcgcat gaatttactc acgctttccg
     1681 acgacgaagc ccgttcgctg ggactgaacg tgacgcgtct gcgctttgta ttgattgtct
     1741 gcgccacact aatcaccgcc agtacggtag caatagcggg cataattggt tgggtcggcc
     1801 tggtcgttcc gcacattgcc cgcttgttga ccggtcataa ccatcagcag ctgttgccca
     1861 tggcaatgtg tactggcgcg attctgctcc tgcttaccga tacgctggca cgcagcattg
     1921 gaacaacaga aatcccactt ggcattctga ccgcttttgt tggcgcaccg tttttcctgc
     1981 ttttgttgtt acgcggaggt cgccagtgac tttgcttgcc gtccgtcatg cgtctctggg
     2041 ttactcccgc catccggtgc tacgcgacgt gtccttcacc ctgtcgcaag gaacaatttg
     2101 ttgtctgtta ggagccaatg gttgcggcaa aaccacactc atgcgctcca ttcttggcgt
     2161 tataccgtta ttaaaaggcg aggtcttact ggatagcctc cctgtgcaga cactcagcca
     2221 tcgccagcgc gcgcaggcta ttgcctgggt gccgcaggcg cacgacggta tttttgcttt
     2281 tagtgtgctg gatatggtgt tgatgggcct ggcccccacc ataggcgctt tttccgttcc
     2341 cggtaagcaa gaacgcctga aagccacgga gcaactggaa aagctgggca ttttgcatct
     2401 ggcggcacga cgctggaata cgcttagcgg cggtgaacgt cagctggctt taatcgcccg
     2461 tgcactcgtc cagcaaccgc gcttgttgtt actggatgag cctgcctcca gcctcgattt
     2521 cggtcatcag atccaactac tggacacgct ggcacagctc aaaaataacg gcatgacaat
     2581 gttaatgtcg actcatcatc cgctccatgc caacgccatt gccgacagca ttatccaggt
     2641 agagccagat ggacgagtaa cacaaggctt accgaccgaa cagttaacaa caaacaaact
     2701 ggctgccctg tatcgggttt ctgccgacca gattcatcat cacctctctg caattagcca
     2761 ctaaggaaaa atcatgctca ttgatgatat tgattttgca gacctttatc tgcagcaact
     2821 caagctggcc caccgcacag aaaaaacgcc ggatcactgg gatcaacggg cggaaaaaat
     2881 ggcggaaaat tgcgcctcgc ctacggacag ctacctgcaa caactgatcg ctaaaattga
     2941 cctacaaggc gctcagacct tattcgatat gggctgcgga ccgggtaccg tatcgctggc
     3001 gctggctgac aaactcacga cgatttatgg cgttgattac agtcagggga tgctgaacgt
     3061 tgccgcccgt cgcgccgccg cgctgaaggc agacaatgtc cacttgatcc agcgcgcctg
     3121 ggaagaggac tggagtgacc tgccacgctg cgatattgcc gtggcttccc gctccactct
     3181 ggtggctgat atgcgccagg caatgagcaa actgaataat caggcacggc ttcgcgtcta
     3241 tacgactcat ctggtcagca cctcgttcgt ctctccagcc attcagcggg cggctggtcg
     3301 ggaagtgatc gaattaccca actatatctt tgcgctcaac gtcctgtatc agatgggaat
     3361 ttatgcccac gttgatttta tccgtggaca aaattgccaa caggataaca gcacctagga
     3421 acgttttgag cagaacgtca gctggagtct tggcgcgctt aacgacgatg agcgtgagcg
     3481 gttatatcgc tggtatcaac aacaagatgc tcgtgccctc gcccctgcca gccgcgactg
     3541 ggcgctcatc tggtgggata gcgtaccgca ggaggcactg cgatgatttt cctttctcag
     3601 gcacaaatcg atgcgttact tctggaagat atccagggcg gtgacctgac cacgagagca
     3661 ttaaatattg gacaccagca tggctatata gagttttttc tccgtcaggg cggctgcgtc
     3721 agcggcgttt ctgtcgcgtg taagatgtta actacgctgg gattaactat tgatgacgcg
     3781 gtcagcgacg gttcacaagc gaacgcgggt cagcggctaa tccgtgcgca gggtaatgcc
     3841 gcagcacttc atcagggatg gaaggcaatc cagaatgtgc tggagtggag ttgcggtgtt
     3901 tctgattatc tcgatcaaat gctggcgtta cttcgtgaac gttatcctga tggcaatatc
     3961 gcctgcaccc gaaaagcaat tccgggcacc cgcttactgg cctcgcaggc aattctggct
     4021 gccggaggac tgattcatcg tgccggatgt gcggaaacca tattattgtt tgccaaccac
     4081 cgccattttc ttcatgacaa tcaggactgg tcaggcgcaa tcaatcagtt acgtcgccac
     4141 gcacccgaga agaaaattgt tgttgaagcc gacacgccga aagaggcaat cgccgcgtta
     4201 cgcgcgcaac cagacgtact tcagctcgac aaatttagtc cgcaacaggc aactgaaatt
     4261 gcgcaaatag cgccgtcgct ggctccccac tgcacgctgg ctctgaccgg cggaattaat
     4321 ctgacgacac tcaaaaatta cctcgactgc ggcattcgcc tctttattac ctccgcgcct
     4381 tattacgcgg caccggctga cattaaagtc agtctgcaac ccgcagcctc tatttaattc
     4441 tttgttaaac ggaatttatt ctatgagact caaaaaacat tatctctgca cagcattatc
     4501 acttgccttt acccagcagg ccgtagcggc tcaggagagc gactcgctga ccgtatggtc
     4561 cagtccggta tcatcgacga cgaccaccgt tctcgatcaa cccaccatga aggccctgga
     4621 taaacagaat gtcgctcagg cattaagtgt cgtccccggc gtggtgctgc aaaagtcagg
     4681 cagccgcaac gaagaacagg taaaagttcg tggctttgat agtcggcagg tgccggtcta
     4741 tttcgacggt gtgcccattt atgttcccta tgacggcaac ctcgatctgg cgcggattct
     4801 gaccaacaat ctgggggcag tcgaagtttc caaagggtat tcgtcgctgc ttcagggacc
     4861 taatcagatg ggcggagcca ttaatatcac cacccagaag ccaacaaaac ctctggaagc
     4921 aagtctggga tatcgccagg gatggagccg tagccaggac aatgcctatg atatgcatgc
     4981 ttcatttgcc gccagcagcg aactggggta tttgcaagtc agcggtagcc agctaaagca
     5041 ggattttctc ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac acggcaagat
     5101 gattaattca tcggctaatg ataaacgcgg cattgtgaag ctcggattta caccacgtga
     5161 aaacgatgaa tacacactga cttacattaa gcaggatggt gaaaaagata acccgccata
     5221 cagcggaaat agtggtcaaa aatcacgcta ctggcagtgg ccagagtatg acaaagaaag
     5281 tttttattat cagggaacga cccaactaaa cgatcgtttt accctgaaaa gtcggctgta
     5341 tcgcgacacc tttgaaaata cgctgatgat gtacaactcg ctggctgatt tgaaaaataa
     5401 aaaaggcagc tacagccatt attccgatta cagcgacggt gccggattac aactggcagc
     5461 cgatgtgcgt gaaaacgatc tgctgacgtt tgccgttaac tggaaagatg acgtacaccg
     5521 ggaaaaaggt gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct ggtcgctcgc
     5581 cagtgaatat caatgggctg ctgccgataa tgtcgatgtt gtggctggaa tcagctatga
     5641 ctggcgcgat agcgtagaag cgaaaaaaca cgagaaagat ggcagcatca cccattatga
     5701 cgacaacaat cagtcagctt ttaactggca ggtgatgggg aaataccact ttgtcaatga
     5761 agacacgctg gcgctttcgt actatgaccg caaacgcttt ccgacgctga aagaacgcta
     5821 taccacatcc aaacctgcgt ataaccagat agcgattgtt aacccgcagc tcaagccgga
     5881 acgcgctcgc ggggtggatt taacctggaa tggtgccttc acgcacgact ggggatttga
     5941 ggtcagcgtt tactataacc gggtgagtga tgccatcctc tcgcacaata tcgatgccga
     6001 taccattcaa aatcagaaca gcggcacggt ggattacagc ggtctggatg ccggtattaa
     6061 ggggaaaatc agcaatatac tggatgtagg attgagctac gccctgatcc acgctgacgc
     6121 caaacgtaaa gacatcggca agataaccga tctgccaacg cagacaatga ccgcatggat
     6181 gactctcaaa ccgtgggagc cgttaagcgt aacgctgtcg gaagaggcgc gttcctccag
     6241 ctacagtaac agtgacggtt cacaaaaagc cgccggtttt gcggtgaccc acattcgagc
     6301 cgattacacc ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc tgtttgatac
     6361 ccaatatgcc tacagtgaag ggtttgttga agaaggtcgc aatttctggg caggtgtcga
     6421 gtatacgttc tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc cttatgaaaa
     6481 tcttattctc ttcgcaggag gcggaatacg cccagaacga gaatagctcc caccaccgcc
     6541 accaggaagc tgtgcaaatt aaaaccgctg atggagccgc caatgccaaa catggtcgcc
     6601 agccagccgc cgaccaccgc accgactatc ccgagaatac aggtcaggaa aaatccacca
     6661 ccatcacgcc ccggcatgat tagcttggcg ataatgccgg ctatcaggcc aaaaataatc
     6721 caggcaataa ttcccatgtt caggccctcg tttcgatgaa tcggacgcaa gaaatgctta
     6781 cgtcaacgct ctaagtatag gcaagggcgg gatcgatgct gggcgtatgt cggctgattt
     6841 aagaacaaca tgtaaaaaat attcacgcat ttgtattaag ttttgttaac tgtgaccgat
     6901 aaaccaaaga cagtttgtca gtcaggagtt tttccgcgtg agtcattacc atgagcagtt
     6961 cctgaaacaa aatccgttag ccgtcctggg cgtgttacgc gatttgcaca aagccgcaat
     7021 tcctttgcgt ctcagttgga ttggcgggca gctgatcagc aaaatactgg taataacccc
     7081 ggataaactg gtgctggatt tcggcagtca agccgaagac aacatcgccg tgctaaaggc
     7141 acagcacatt accattaccg ccgaaactca gggtgcgaaa gtcgagttta ctgttgaaca
     7201 actacagcag agtgaatact tgcagcttcc ggcatttatt accgtaccgc ctcccacctt
     7261 atggtttgta caacgacgcc gatatttccg catctccgcc ccactccatc cgccttattt
     7321 ttgccagacc aaactggcgg ataacagtac gttacgtttc cgcctgtatg atttgtcgtt
     7381 aggcggcatg ggcgcattac tggaaacagc aaaacctgcc gggttgcacg aaggtatgcg
     7441 cttcgctcag attgaagtca acatggggca atggggtgtt tttcactttg acgcccagtt
     7501 aatctccatc agcgagcgca aagtga
//
LOCUS       DAZFKY010000202         7514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFKY010000202 DAZFKY010000000
VERSION     DAZFKY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..347
                     /gene="chaB"
                     /locus_tag="ACHKCL_004085"
     CDS             117..347
                     /gene="chaB"
                     /locus_tag="ACHKCL_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5764949.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            508..1203
                     /gene="chaC"
                     /locus_tag="ACHKCL_004086"
     CDS             508..1203
                     /gene="chaC"
                     /locus_tag="ACHKCL_004086"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5764950.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1247..1600)
                     /gene="ychN"
                     /locus_tag="ACHKCL_004087"
     CDS             complement(1247..1600)
                     /gene="ychN"
                     /locus_tag="ACHKCL_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5764951.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1786..3180
                     /gene="ychO"
                     /locus_tag="ACHKCL_004088"
                     /gene_synonym="iatA"
     CDS             1786..3180
                     /gene="ychO"
                     /locus_tag="ACHKCL_004088"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5764952.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3181..3831)
                     /gene="narL"
                     /locus_tag="ACHKCL_004089"
     CDS             complement(3181..3831)
                     /gene="narL"
                     /locus_tag="ACHKCL_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5764953.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3824..5620)
                     /gene="narX"
                     /locus_tag="ACHKCL_004090"
     CDS             complement(3824..5620)
                     /gene="narX"
                     /locus_tag="ACHKCL_004090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5764954.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5959..7350
                     /gene="narK"
                     /locus_tag="ACHKCL_004091"
     CDS             5959..7350
                     /gene="narK"
                     /locus_tag="ACHKCL_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5764955.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WGPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1746 a         1838 c         1980 g         1950 t
ORIGIN      
        1 ggcaatgttt gttttagcgt ttcttaagaa cagtctgaca tgaaaattct tatgtttttg
       61 caagagtaga tattgttgac cacacttaat gttcaacttt gtaaaaggag tcaacgatgc
      121 cgtataaaac gaaaagcgac ctgccggaaa gcgtaaagca cgttctaccg tctcatgccc
      181 aggatatcta taaagaagcg ttcaacagcg catgggatca atataaagat aaagaagatc
      241 ggcgtgatga cgccagtcgc gaagaaaccg cgcataaagt ggcctgggct gctgtgaagc
      301 atgaatatgc caaaggggat gatgataaat ggcataaaaa atcgtaaaac cggtagctta
      361 gttaaagcta ttcgtgcggt gttgccttgc aagtggtccg cggattgcat attgtcccgt
      421 tagtggtttc aaaatgagca gtaaaaatgt ccggaagaca ccaaaaagtt gtcgcaggga
      481 agcatgcagt ggcggaggtg taagaaggtg ataacgcgtg atttcttgat gaatgccgat
      541 tgtaaaacgg catttggtgc cattgaagaa tcactcttat ggtcagcaga acaacgggcg
      601 gcttcgctgg cggcgacgct ggcttgtcga cctgatgaga gaccggtgtg gatcttcggt
      661 tatggatcgt tgatgtggaa tccggcactg gagtttaccg agtcgtgcac cggtacactg
      721 gttggatggc atcgcgcgtt ttgcctgcgc ctgaccgccg ggcgcgggac tgcgcaccag
      781 ccgggacgga tgcttgcact aaaagagggc ggacgtacca caggcgtcgc ctatcgactg
      841 ccagaagaga cgctggagca ggaactaacc ctgttgtgga agcgcgagat gattaccggc
      901 tgttatctgc caacctggtg tcagcttgat cttgatgatg gacgcacagt aaacgccatt
      961 gtgtttatta tggacccgcg acatccagaa tatgaatctg acactcgcgc tcaggtcatc
     1021 gcgccgttga ttgcggcggc gagcggtccg ctgggaacca acgcacaata cctgttttca
     1081 ctggaacagg agcttatcaa actgggaatg caggacgatg ggctgaatga tttgctggta
     1141 tcggtaaaaa aactgctggc ggagaattat ccggatggtg tgttgcggcc gggattcgcc
     1201 tgagtaaact tccctcatcg tggggcgtca gacgccccct caaacattaa aatgtgagca
     1261 ctttatcggc tgacagcgtc cattgcgcca gttccacaag agtaccgatt tccaccccat
     1321 caatcagagg aagtgtacta atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg
     1381 gtacattctg agcggtaagg atctccagca tttgctgtat gttgtagcct tcccctggtt
     1441 tttgcccgcg caacccggct gtgaccgcat cagacatcag gaacagacgc agatccagat
     1501 tgctctcctg ctctcgtaac gcaatggcca gccgcaagct gttaaacaag gattcgctcc
     1561 cgtaaggtgc gccattggca acgatcacga ttttttgcat tatttactcc tgtattcagg
     1621 gaattagaca ctcatcttct atcttactgc ttctgcagcg tctgaccaat cggtcacatt
     1681 tttaaggatt ttcctgaaag cgcgagaaaa tacgacaaaa gttgccagta atcgttattc
     1741 tttaaggcta tgttttttca ttttttaccg gaagttaccg acgttttgag ccgtttcgtt
     1801 cctcgcatta ttccgtttta tttactcttg ctggcggcag gcggtacagc taacgcacaa
     1861 tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat
     1921 ttaggcatgg cgcctgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat
     1981 ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc
     2041 gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcgtgg
     2101 ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg tcattttacc
     2161 ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc
     2221 cagcttggtc ttactcagca ggatgatggg ctggtgagca atgtgggcgt tgggcaacgc
     2281 tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt gctggatgaa
     2341 aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg actatcggca
     2401 aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga acaacggatg
     2461 gcgcgcgggt acgacctgac agcccggatg cgcatgccgt tctatcaaca cctcaatacc
     2521 agtgtcagcg tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt
     2581 tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact
     2641 gtgacggccc agcataaaca gggtgaaagt ggcgagaatc aaaataacct cgggctgaat
     2701 cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga ggttgccgaa
     2761 agtcagtcgt tacgtggtag tcgctatgat aatccgcagc gaaataatct accgactctt
     2821 gagtaccgac agcgaaaaac gttaacggtg tttcttgcga caccgccgtg ggatctaaaa
     2881 cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg
     2941 atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg
     3001 gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcgag caatcactgg
     3061 cgattgtcgg tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg
     3121 ctaacgcttg tcgaaccgtt cgacgcattg tcaaacgacg aactgcgctg ggaaccgtaa
     3181 tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat
     3241 tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatgt ccaggcggcg
     3301 ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgtgg
     3361 ggttaactgg ttaacatcgc gctcagtcgt ggcacggtta gcgcgcaagc tggcggccag
     3421 aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt
     3481 cagcagatct tccggttcca tatcttttaa cagatagcca tccgcgccgc gtttcagtgc
     3541 ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc ctgagaggga
     3601 cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa
     3661 caggatcaga tcgggatcaa gagattccgc cagttcaata ccctgttcgc cattactcgc
     3721 ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag
     3781 catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca tgggtatctc
     3841 cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg
     3901 attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta
     3961 tcatgccgta gtgattgctg cggatggcat tttcaggcac gccgcagccg ttatcctgga
     4021 cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacctca ctcgcttgcg
     4081 aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat
     4141 gcgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg
     4201 cgctgtactc ttcgcaactc gcctccagcg ccggacgtaa tccaggctcg gtgagctgca
     4261 agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga
     4321 tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac
     4381 tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa
     4441 tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgc cgatccagcg
     4501 ccagcgtggc ggtgagttgt tcaaccaggg tatccaccag ttgttgttga tcatggctaa
     4561 gatgacgccc ctgcggtagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca
     4621 gccgccactt cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct
     4681 ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga tgattctctt
     4741 catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc
     4801 cgttgagtac aggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa cggcggttag
     4861 cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa
     4921 cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag
     4981 ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat
     5041 ggctgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca
     5101 accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca
     5161 gtaccactgt ctcaatgcgc atttccgtgg tgcggtcaaa accagatacc agctgatcaa
     5221 gcccggcaac aaactggctg acatccgctg acaccgtttc gcggttttgt gcacgcatca
     5281 gtgcagggat gagttcatta cgccagtaat cttgtaaacc ctgtaattgc gccagttgtc
     5341 cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa
     5401 ttaagggctt gtctttctcg cttaatggca ctgctgccaa cagacggtaa ctttgcatgc
     5461 gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc
     5521 cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct
     5581 gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacctct tccttcaggc
     5641 tttaaatgag caataacctt aatgaatgtg acgatacatt ctggaatggc agtattctcg
     5701 gctattggct gaagtatacc catacccgga aagagttact ccttatttgc cgtgtggtta
     5761 gtagctttac atcggtaagg gtagggattt tacagcaccg tgaaaaatct cataattttt
     5821 atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt
     5881 atcgtttgat ttacatcaaa ttgcctttag ctacagacac taaggtggca gacatcgaaa
     5941 cgagtatcag aggtgtctat gagtcactca tccgcccccg aaagggctac tggagctgtc
     6001 attacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc
     6061 agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg
     6121 ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac cgatcagcta
     6181 tttatgttga ctgcgttgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt
     6241 atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt
     6301 ccttgtgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc
     6361 atcatctctc tgctgtgcgg ctttgctggc gcgaacttcg catccagtat ggcaaacatc
     6421 agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gcctgaatgg tggtctgggc
     6481 aacatgggcg ttagcgttat gcagttggtt gctccgctgg tggtatcact gtcgattttc
     6541 gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat
     6601 gcgtcctgga tatgggggcc gttccttgcc atcttcacca ttgcggcgtg gtttggcatg
     6661 aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt
     6721 catctgtgga ttatgagcct gctgtatctg gcaaccttcg gttccttcat cggcttctcc
     6781 gcgggctttg cgatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct
     6841 ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt
     6901 ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg
     6961 ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc
     7021 ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttcc
     7081 gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt
     7141 gcgatgcgtg aagcggcaac cgacacggcg gcggcgttgg gtttcatctc tgcgattggc
     7201 gcgattggtg gcttctttat cccgaaagcg ttcggtagct cgctggcatt aacgggttcg
     7261 ccagtcggcg caatgaaagt atttttgatt ttctatatcg cctgcgtagt gattacctgg
     7321 gcggtatatg gtcggcattc taaaaaataa atcttaatat ttgattatcc tttgcgcggc
     7381 atgatgtcgc gcttttttta tgcgtcattt agttacaaca tactaatgtt atatggttta
     7441 tttcgccgga tttcattaag aaccattaat atgttaccca tggggaatac tccttaatac
     7501 ccatttgcat aaaa
//
LOCUS       DAZFKY010000203         7473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFKY010000203 DAZFKY010000000
VERSION     DAZFKY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            214..1842
                     /locus_tag="ACHKCL_004092"
                     /pseudo
     CDS             214..1842
                     /locus_tag="ACHKCL_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
     gene            complement(1842..1964)
                     /locus_tag="ACHKCL_004093"
                     /pseudo
     CDS             complement(1842..1964)
                     /locus_tag="ACHKCL_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3262
                     /gene="dhaK"
                     /locus_tag="ACHKCL_004094"
     CDS             2192..3262
                     /gene="dhaK"
                     /locus_tag="ACHKCL_004094"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5764956.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3273..3905
                     /gene="dhaL"
                     /locus_tag="ACHKCL_004095"
     CDS             3273..3905
                     /gene="dhaL"
                     /locus_tag="ACHKCL_004095"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5764957.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3916..5334
                     /gene="dhaM"
                     /locus_tag="ACHKCL_004096"
     CDS             3916..5334
                     /gene="dhaM"
                     /locus_tag="ACHKCL_004096"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5764958.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            5654..7338
                     /gene="treA"
                     /locus_tag="ACHKCL_004097"
                     /pseudo
     CDS             5654..7338
                     /gene="treA"
                     /locus_tag="ACHKCL_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         1975 a         1843 c         1868 g         1787 t
ORIGIN      
        1 taactcgatc taaactcacc tttgtaaggg ggatgttaaa tatatattaa tttctgtgtt
       61 tctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      121 caaatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      181 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      241 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      301 tttggcgcta ttggtttgaa tgcaattctc ttttcccccc tgctgatagc tgctgataca
      361 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac tggagatacc
      421 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      481 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      541 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      601 ggtcaaacct ctgccatcgg cattaattta attggtgact atacccatgc tgacttaggc
      661 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      721 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      781 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      841 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
      901 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
      961 gtcgatctcg gaacaaacag ttccattaaa accagtggcg ataatgcaca cggcctctgg
     1021 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1081 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1141 cagggcggtg gtctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1201 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1261 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1321 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1381 gcaggagcca gggggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1441 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1501 gccgccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1561 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1621 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1681 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1741 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1801 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaagccctg ggcctgatgt
     1861 ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga ggttgcaatt
     1921 aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg catcccttat
     1981 gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt ttcaatatgg
     2041 aacattcatt aaagcatatg tttcactatg aaacataatt tgggctaatt ttcttttccc
     2101 ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc
     2161 catgccctac cgtaattgct ggagcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga
     2221 cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga cactgcatca
     2281 ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc
     2341 gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg ggatgctttc
     2401 gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct ttgaatgcgc
     2461 catgcaagtt gatggcggcg aaggtgtact gttgattatc aaaaattaca ccggcgatat
     2521 tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag tgaccactgt
     2581 ggtcattgat gacgacgttg cagtaaaaga cagtctttat accgccgggc ggcgcggcgt
     2641 tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact
     2701 ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc
     2761 tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttaccctgg cggataatga
     2821 gatggagttt ggcgtcggca ttcatggtga gccgggtatt gaccgtcgcc ccttctcttc
     2881 ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaaaatggct cataccatcg
     2941 cactttgcgt ttctgggatt atcaacaagg cagttggcag gaagaaccac aaaccaaaca
     3001 accgctccag tctggcgacg gggtgattgc gctggttaac aatcttggcg caactccact
     3061 ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag cgggattgac
     3121 tatcgagcgt aatttaattg gcgcgtactg cacctcactg gatatgaccg gtttctcaat
     3181 cactttactg aaagttgatg acgaaacgct ggcactctgg gacgccccgg tccacacccc
     3241 ggcccttaac tggggtaaat aaggagaaag caatgtcact gagcagaact caaattgtta
     3301 actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt accggactgg
     3361 atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg
     3421 tggaaaaact ccctgctatt gcagataaag atatcggttt cattctcaag aataccggta
     3481 tgacgctgct ttcctgcgtc ggtggtgcca gtggtccgct gttcggtacc ttctttatcc
     3541 gcgccgcaca ggcgacccag gcacggcaaa gcctgacgct ggaagagctt tatcagatgt
     3601 tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc gataaaacca
     3661 tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag caaaatctct
     3721 ctgttccggc ggcgctcgaa gctgccagta gcatcgccga attcgctgca caaagtacga
     3781 ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt attggtcacc
     3841 aggatcccgg cgcaacctcg gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag
     3901 agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg actgggagaa
     3961 ggtgtcggtg aattagcccg tcagatgtta atgagtgata attgtaaaat cgccattgct
     4021 gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc
     4081 atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg tagcgcatta
     4141 ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg
     4201 tgtgctgcgc cgttggtcga aggtacactg gcagcaactg tcagcgcggc ctcgggggcg
     4261 gagatcgaca aagttatctt tgacgccatg catgcgctgg aagccaaacg tgaacaactg
     4321 ggtttaccgt cctccgacac tgaaatctct gactcatgtc ctgcgtacga tgaagaagcc
     4381 cgttctctgg cggtggtcat taaaaaccgt aacggcctgc acgtccgtcc ggcctcccgg
     4441 ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc
     4501 gtcacaccgg agagtattaa ccagattgcg ttactacaag ttcgctataa cgatacgctg
     4561 cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg tcagctggct
     4621 gaagataact ttggtgaaac ggaggaagtt gctccaccta ctctgcgtcc cgttccgcct
     4681 gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc aaaatcaccc
     4741 ctgaccgtgg aagaagaaca ggaacgatta cgccaggcca ttgacttcac gttattagat
     4801 ctgatgacgt taacagcaaa agcagaagcc agcgggcttg acgatattgc cgcaatcttt
     4861 tctggtcacc atacactgtt agatgatccg gaactgctgg cggcggcaag cgaactcctt
     4921 cagcacgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga acttagccag
     4981 caataccagc aactggatga tgaatatcta caagctcgct atattgatgt ggacgatctt
     5041 ctgcatcgca ccctggtcca cctgacccaa acgaaagaag aactcccgca gtttaactcg
     5101 ccaactattc tactggcgga gaacatttat ccctccccag tactgcaact ggatccggcg
     5161 gttgtaaaag gtatctgcct tagcgccgga agtccggtat cccacagcgc cctgatcgcc
     5221 cgtgaactgg ggattggctg gatttgccag cagggtgaga aactgtatgc gatacaacca
     5281 gaagaaaagc taacgctgga cgttaaaacg caacgtttca accgtcaggg ttaagccacc
     5341 gttcggatgg catcgttctg atgtcatccg gcaatttact cagcactaat gttttttaac
     5401 tacctgttat atttttcacc aatttatgat gatttttctt cgctaatcgc gaaccaaacg
     5461 acgctggctg gctaataaac agacgttatt tgatcactct atttcgccgt tatttatccc
     5521 tttaattccc ttttctaaaa tgcctgacag ttcgcagaat gagatttcga tcatgcagct
     5581 agtgcgatcc tgaactaagg ttttctgata cttgaatacc gttttattcg gtttcgccta
     5641 aggagaatga ttgatgaaat cccccgcacc ttctcgcccg caaaaaatgg cgttaattcc
     5701 agcctgcatc tttttgtgtt tcgctgcgct atcggtgcag gcagaagaaa caccggtaac
     5761 gccacagccg cctgatattt tattagggcc gctgtttaat gatgtgcaaa acgccaaact
     5821 ttttccggac caaaaaacct ttgccgatgc cgtgccgaac agcgatccgc tgatgatcgg
     5881 atgcagcaaa accagagcgg atttgatctg cgccatttcg ttaacgtcaa tttcaccctg
     5941 ccgaaagagg gcgagaaata tgttccgcca gagggacagt cactgcgcga acatattgac
     6001 ggactttggc cggtattaac gcgttctacc gaaaacaccg aaaaatggga ttctctgtta
     6061 ccgctgccgg aaccttatgt cgtgccaggc ggacgttttc gcgaggtata ttactgggac
     6121 agttacttca ccatgttagg acttgccgaa agcggtcact gggataaagt cgcggatatg
     6181 gtggccaatt ttgctcatga aatagacact tacggtcata ttcccaacgg caaccgcagt
     6241 tactatttaa gccgctcgca gccgcccttc tttgccctga tggtagagtt actggcgcag
     6301 catgaaggcg atgccgcatt gaagcaatac ctgccgcaaa tgcaaaaaga atatgcttac
     6361 tggatggacg gtgttgaaaa cctgcaagcc ggacaacagg aaaaacgcgt tgtaaaactt
     6421 caggatggta cccttctcaa ccgctactgg gacgatcgcg atacgccacg accagagtca
     6481 tgggtggaag atattgccac cgctaaaagc aatccgaatc gacctgccac tgaaatttac
     6541 cgcgacctgc gctctgctgc tgcgtctggc tgggatttca gctcgcgctg gatggacaat
     6601 ccgcagcaac taaatacctt acgcaccacc agcatcgtac cggtcgatct gaacagcctg
     6661 atgttcaaaa tggaaaaaat ccttgcccgc gccagcaaag ctgccggaga taacgcgatg
     6721 gcaaaccagt acgaaacact ggcaaatgcc cgtcaaaaag ggatcgaaaa atacctgtgg
     6781 aacgatcaac aaggctggta tgccgattac gacctgaaaa gtcataaagt gcgcaatcag
     6841 ttaaccgcgg ccgccctgtt cccactgtac gtcaatgcgg cagcgaaaga tcgcgccaac
     6901 aaaatggcga cggcgacgaa aactcatctg ctgcaacccg gcggcctgaa caccacgtcg
     6961 gtgaaaagtg ggcaacaatg ggatgcgcca aatggctggg caccgttaca gtgggtcgcg
     7021 acagaaggat tacaaaacta cgggcaaaaa gaggtggcga tggacattag ctggcacttc
     7081 ctgaccaatg ttcagcacac ctatgaccgg gagaaaaagc tggtggaaaa atatgatgtc
     7141 agcaccaccg gaacgggggg cggcggtggc gaatatccat tacaggatgg ctttggctgg
     7201 accaatggcg tgacgctgaa aatgctggat ttgatctgcc cgaaagagca accgtgtgac
     7261 aatgttccgg cgacgcgtcc actcagtgaa tcaacaacac agccggtaaa acaaaaagag
     7321 gcggaaccta cgccttaact ttgctgtata tcgagcaatg ccattatgtg cataaaattg
     7381 gcattgctct tttttcgcca cgaaatgccc cccattctac ggtctatgct tttattttct
     7441 tatatcagag aagattttaa ttttatttta ttt
//
LOCUS       DAZFKY010000204         7431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFKY010000204 DAZFKY010000000
VERSION     DAZFKY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            281..799
                     /locus_tag="ACHKCL_004098"
     CDS             281..799
                     /locus_tag="ACHKCL_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5764959.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1009..3150
                     /locus_tag="ACHKCL_004099"
     CDS             1009..3150
                     /locus_tag="ACHKCL_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5764960.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3226..>7431
                     /locus_tag="ACHKCL_004100"
     CDS             3226..>7431
                     /locus_tag="ACHKCL_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO5764961.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHD
                     ERTHHYHYDSQHRLVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPE
                     RKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDE
                     ESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSP
                     SDPIYKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEG
                     MPVVLFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGK
                     DNGVFELVPDEKQPGDFHKFTPTG"
BASE COUNT         1755 a         1919 c         2369 g         1388 t
ORIGIN      
        1 atatttattc atgattcagc tttggccatt ttatggaggc aatacggact ttgaacagtt
       61 gggtatggct atccagatat atattattaa gttattgctg aataagagat ttaatgattt
      121 taaaaatagt ttttacgaca accgtaaatt ctagctcggt ttgtatgtaa aaaatactga
      181 aaaatgatgc ggttccgtga agtgagctgc atctgcctgt ctgggattaa cggcaggtca
      241 ttgtaagtag taattaacca cgttaacaag gagtttacaa atgccaacac cgtgttatat
      301 ctccatcact ggtcagaccc agggaaatat tactgcaggt gcatttaccg ctgattctgt
      361 cggcaacatc tatgtgcagg ggcacgaaga cgagatgctg gtgcaggaat tcctgcataa
      421 cgttactgtc ccgaccgatc ctcagtccgg tcagcctgcg ggtcagcgtg ctcacaagcc
      481 tttcattttt accgtggcgc tgaacaaagc ggttccgctg atgtacaacg cactggcctc
      541 cggcgaaatg ctgccaacca ctgaacttca ctggtggcgt acctctgtgg aaggcaaaca
      601 ggagcattat ttcactaccc gcctgactga ttcgacgatt gtcgatatga aactgcatat
      661 gccgcattgc caggacccgg cgaaacgcga gttcacccag ttgctcgaag tttcactggc
      721 ctaccgcaaa attgaatggg aacacgtcaa atctggcacc tccggtgccg acgactggcg
      781 cgcaccgctg gaagcataag ttaagccaac agcatccggc tatcaggccg gatgctgttt
      841 ttgtattacg ccgtagaaag aaaggttgtc agtaaggggt tacgttcacg ggagatggcg
      901 gctgccggaa acttcggcgg tagcgtgcct gaacgcaagc gtagcactgt actgtcacag
      961 ccgctttaac agcgaactgt ctgtttttcc ttaaccccgg aggttgctat gtcaaccgga
     1021 ttacgtttca cgctggaagt ggacggcctg ccaccggatg cttttgcggt ggtctccttt
     1081 catctgaacc agtcactctc ttcgcttttt tccctcgctc tctctctggt cagccagcag
     1141 tttctctccc ttgaattcca gcagatcctc gacaaaatgg cctacctgac gatatggcag
     1201 ggcgatgacg tacagcgccg ggtgaaaggt gtggtgacct ggtttgaact gggggagaac
     1261 gacaaaaacc agaagctgta cagcatgaag gtgtgcccgc cgctgtggcg cacagggctg
     1321 cgccagaact tccgtatctt ccagaatgag gacatcgcaa gcatcctcgg cacgatattg
     1381 caggaaaacg gggtgaccga gtggagcccg ctgttcagcg agccacatcc ttcccgtgag
     1441 ttttgtgtcc agtacggtga gactgattac gatttcctgt gccggatggc ggcggaggaa
     1501 ggcatcttct tttatgagga gcacgcgcaa aaaagtaccg accagagcct ggtcctgtgc
     1561 gataccgtgc tttatctgcc ggagtccttt gagatcccct ggaatccgaa cacccgtacc
     1621 gaggtaagca ccctctgcat cagccagttt cgctacagcg cacaaatccg cccttcttcc
     1681 gtggtgacca aagactacac ctttaaacgc cccggctggg ccggacgttt tgaccaggaa
     1741 ggccagcacc aggattacca gcgcacacag tatgaagtgt atgactatcc cggacgtttc
     1801 aagggcgccc acgggcagaa ctttgcccgc tggcagatgg acggctggcg aaacaacgca
     1861 gaagtggcgc gcggaacaag ccgttcgccg gagatatggc cgggacggcg aattgtgctg
     1921 acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg tggtggcaag tgaactgcac
     1981 ggtgagcagc cgcaggcggt gccgggacgc aggggttcag gcaccacgct ggataaccat
     2041 tttgcggtaa taccggcaga cagaacatgg cgaccacagc cgttgctgaa accgctggtg
     2101 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgaa
     2161 catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa ccaggacagt
     2221 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg ggtttggtaa cctggcgata
     2281 ccgcgtgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga ccagccgatc
     2341 attatggggc gtacctacca ccaggaaaac cgcacacccg gtagcctgcc ggggacgaag
     2401 acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga actgaagttt
     2461 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
     2521 gtgctgaata accgcaccac ggatgtgata aacaaccatg ccgaaaaaat aggtaacaac
     2581 caggcgatca ccgttaccaa taaccagatc ctgaacattg gcgttaatca gatacagacg
     2641 gttggtgtca accaggtgga aacggtaggc agtaaccaga ttatcaaagt gggatcaaac
     2701 caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggcgta tcagacgacg
     2761 gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt agggctgcat
     2821 aaatcactga tggtgggaat gggctacagc gtcaatgtag ggaataacgt caccttctcg
     2881 gtgggcaaga cgatgaagga aaacaccgga caaacagcag tttattctgc cggtgagcat
     2941 cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag catatttctt
     3001 aatggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc gccggtgatt
     3061 aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa agatttaccc
     3121 cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaatcaaa acggaactgg
     3181 tgcagtgacc agataaacca tattaaaagc agaaggaatt gattcatggg aggaaaaccg
     3241 gcggcgcggc agggtgacat gacccgcaag ggactggata ttgtgcaggg ttcagcaggg
     3301 gtgctgatag gtgcgccgac gggcgtggcc tgctcggtgt gtccgggagg gattacctat
     3361 gctaacccgg tgaacccggt gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg
     3421 ctgcccggcc cgctgccgtt tattctttcc cgcgcctaca gcagctaccg gaccagaaca
     3481 cccgcgccgg tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag
     3541 atacgcgatg aaggcctgat actcaacgac aacggcggca ggagcattca ctttgagccg
     3601 ctgtttcccg gcgagataag ctacagccgc agcgagtcgt tctggctggc gcggggcggg
     3661 gtggcggagc agcacagttc gcagccgcta agcgcgctct ggcaggtgct gccggaagat
     3721 gttcgcctga gtccgcatat gtacctggcg acaaacagcc ttcaggggcc gtggtggata
     3781 ctcaactggc cggagcgggt gccgggggcg gacgaggtgc tgccgccgga gccgcccgca
     3841 taccgggtgc tgacgggcgt ggtggatggc ttcgggcgga cgctggcctt tcaccgggcg
     3901 gcggagggtg atgtggcggg cgcggtgacg ggggtgacgg acggcgcggg ccgccgtttt
     3961 cacctggtgc tgaccacaca ggcacagcgg gcggaagtat tccgtaaaca gcgcgccacg
     4021 tctttatctt cccctgccgg tccccgttct gcttcctctt ctctggtttt ccccgacacg
     4081 ctgcccgccg gtacagaata cggtgccgat aacggtatcc ggctggaggc ggtatggctg
     4141 acacacgacc cggcataccc ggatgaactg cccgccgcgc cgctggcgcg ctacacgtac
     4201 acggccagcg gagaactgcg ggcggtgtat gaccgcagcg ggacgcaggt gcgcgggttt
     4261 gcttatgatg cggagcacgc cgggcggatg gtggcgcacc attatgcggg taggccggag
     4321 agccgctacc ggtatgatga taccggccgg gtgacggagc tggtcaaccc ggaggggctg
     4381 gactaccgct ttgagtacgg gcaggaccgt gtgaccatca cggacagcct gaaccggcgg
     4441 gaggtgctgt acacggaagg cgagggtggc ctgaaacgtg ttgtgaagaa ggaacatgcg
     4501 gacgggagca tcacccgcag cgagtatgat gaggcgggga ggctgaaggc acagacggat
     4561 gcggcgggac ggcggacgga gtacagcctg catatggcgt cgggtgcggt gacagcggtg
     4621 acggggccgg acggcaggac ggtgcggtat ggctataaca gccagcggca ggtgacgtca
     4681 gtgacgtacc cggacgggct gcgcagcagc cgggagtatg atgagaaggg aaggctggcg
     4741 gcggagacct cgcgcagcgg agagacgacg cggtacagct atgatgaccc ggcgagtgag
     4801 ctgccgacag ggatacagga cgcgacgggc agtacaaaac agatggcatg gagccgttac
     4861 ggtcagctgc tgacctttac ggactgctcg gggtacacga cgcggtatga gtatgaccgg
     4921 tacggtcaac aaatcgccgt tcaccgggaa gaaggcatca gcacttacag cagttataac
     4981 ccgcgtggcc aactggtcag tcagaaggat gcgcaggggc gtgaaacccg ttatgagtac
     5041 agcgccgcag gcgacctcac cgctatcgtt gccccggacg gcagccgcag tgagatacag
     5101 tatgatgcgt ggggaaaggc cgtcagcacc acgcagggcg gtctgacgcg cagcatgggg
     5161 tatgacgctg ccgggcgcat caccgtgctg accaacgaga acggcagcca gtccacgttc
     5221 cggtatgacc cggtggacag gctgactgaa cagcgcggtt ttgatggccg gacgcaacgt
     5281 taccactatg acctgaccgg aaaactcacg cagagtgaag acgaggggct tgtcaccctc
     5341 tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag
     5401 cagtggcagt atgatgagca cgggtggcta accaccctca gccataccag tgaaggccac
     5461 cgggtgtcgg tccactacgg ctatgacgat aaaggccgcc tgacgggtga acggcagacg
     5521 gtggagaacc cggagacggg ggagatgctg tgggagcatg agacggggca cgcgtacagc
     5581 gaacaggggc tggcgacccg tcaggagccg gacggtctgc cgccggtaga gtggctgacg
     5641 tatggcagcg gttatcttgc ggggatgaag ctgggcggaa cgccactggt cgagtacatg
     5701 cgggaccggc tgcaccgtga gacggcccgc agcttcggcg gggaggcata tgaacttgcc
     5761 accgcctgga ataccagcgg ccagctccgg agcaggcacc tgaaccttcc gcagcttgac
     5821 cgtgactacg actggaacga caacggacag ctaatccgca tcagcggccc gcaggagagc
     5881 cgggagtacc gttacagtga cacgggaagg ctgacgggcg tccacaccac ggcagcgaac
     5941 ctggatatcg atatcccgta tgcaacggac ccggcaggaa accggctgcc ggacccggaa
     6001 ctgcacccgg acagcaccct cacggcatgg ccggataacc gcatcgcgga agatgcgcac
     6061 tatgtctacc gctacgatga atacggcagg ctggcggaga agacggaccg catcccggaa
     6121 ggggttatcc ggatgcacga cgagcgcacc caccactatc actacgacag ccagcaccgc
     6181 ctggtgttcc acacgcggat acagcacggc gaaccacagg tggagagccg gtacctctat
     6241 gacccgctgg gccgccggac gggaaaacgg gtgtggcggc gggagcgtga cctgacgggg
     6301 tggatgtcgc tgtcgcgtaa accggaggag acctggtacg ggtgggacgg tgacaggctg
     6361 accactgtac agacccaaca gacaagaatc cagacggtat accagccggg aagcttcacg
     6421 ccgctcctga gaatcgaaac agagaatggt gaacaggcga aggcgcggca ccgtagcctg
     6481 gcggaggtgt tgcaggagga cacgggtgtg acgctaccgg cggagctggc ggtgatgctg
     6541 ggaaggctgg agcgggagct gcggcagggc agcgtgagtg aagaaagcca gcagtggctt
     6601 gcgcagtgcg ggctgacggc ggagcagatg gccgcgcagc tggaggcgga atacatcccg
     6661 gagaggaaac ttcatcttta ccactgcgac caccggggac tgccgctggc gctcatcagc
     6721 ccggaagggg aaacggcgtg gcagggggag tatgacgagt ggggaaacct gctgggcgaa
     6781 accagcgcgc agcaccttca acagtcactc cgtctgccgg ggcagcagta tgatgaggag
     6841 tcggggctgt actacaaccg caaccggtac tatgatccgt tgcaggggag atatatcacc
     6901 caggacccga tagggctgga ggggggatgg aacctgtatc agtacccact caatcctatt
     6961 gaacatatag atccgttggg gttagcactt gatttgaatt attattctcc gtcagatcct
     7021 atatataaag gttctcttaa tgtaagggaa tttccaacgg gttttacagt tggaggtcat
     7081 ggttcgccta catctatgag tgatgacaga atcaaaaaag gaagtgatct gacaataaaa
     7141 caattagcta gcgacataag agcgaatcct aaatatcatg aaggtatgcc tgtagtcttg
     7201 ttttcctgcg aaacaggaaa aggcaaaaat tcatttgcac agaaactagc taacgaactt
     7261 gatgctacag tgattgctcc tgatgaaata atatggattt ggccggatgg aaactatgct
     7321 attatggggc aaacagctag gataacaata ggcggtaagg acaacggggt gtttgaattg
     7381 gtgccagacg agaaacaacc aggggacttt cataagttta cacctacagg a
//
LOCUS       DAZFKY010000205         7301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFKY010000205 DAZFKY010000000
VERSION     DAZFKY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..232
                     /gene="ydgT"
                     /locus_tag="ACHKCL_004101"
     CDS             17..232
                     /gene="ydgT"
                     /locus_tag="ACHKCL_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5764962.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            318..758
                     /gene="ydgK"
                     /locus_tag="ACHKCL_004102"
     CDS             318..758
                     /gene="ydgK"
                     /locus_tag="ACHKCL_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5764963.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            835..1416
                     /gene="rsxA"
                     /locus_tag="ACHKCL_004103"
     CDS             835..1416
                     /gene="rsxA"
                     /locus_tag="ACHKCL_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5764964.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1416..1994
                     /gene="rsxB"
                     /locus_tag="ACHKCL_004104"
     CDS             1416..1994
                     /gene="rsxB"
                     /locus_tag="ACHKCL_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5764965.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            1987..4209
                     /gene="rsxC"
                     /locus_tag="ACHKCL_004105"
     CDS             1987..4209
                     /gene="rsxC"
                     /locus_tag="ACHKCL_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5764966.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARLARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAITRAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED
                     "
     gene            4210..5268
                     /gene="rsxD"
                     /locus_tag="ACHKCL_004106"
     CDS             4210..5268
                     /gene="rsxD"
                     /locus_tag="ACHKCL_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5764967.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5272..5892
                     /gene="rsxG"
                     /locus_tag="ACHKCL_004107"
     CDS             5272..5892
                     /gene="rsxG"
                     /locus_tag="ACHKCL_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5764968.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            5896..6591
                     /gene="rsxE"
                     /locus_tag="ACHKCL_004108"
     CDS             5896..6591
                     /gene="rsxE"
                     /locus_tag="ACHKCL_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5764969.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            6591..7226
                     /gene="nth"
                     /locus_tag="ACHKCL_004109"
     CDS             6591..7226
                     /gene="nth"
                     /locus_tag="ACHKCL_004109"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5764970.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1683 a         1878 c         1944 g         1796 t
ORIGIN      
        1 attttatgga ccttttatga ctgttcagga ctacttatta aaatttcgca aaatcagttc
       61 actcgaaagt ctggaaaaac tctacgacca tcttaattac accctgacgg acgatcagga
      121 actgatcaat atgtatcgtg ctgccgatca ccgtcgcgca gagctggttt ctggcgggcg
      181 tttgtttgac ctcggccagg taccgaagtc cgtctggcac tatgttcaat aaagaaagta
      241 gcgatcggtt ataaaagcct tataataacg cccctaaaat tagatttgcc tgtttgcgtc
      301 gctattctgg agaacctatg accaccacaa cgccacaacg tattggaggc tggttgcttg
      361 gcccgttggc ctggctttta gtcgctctgt taagtacgac gctggcgttg ttgctgtata
      421 ccgctgcgtt atcttctccg caaacatttc aaacgctcgg cgcacaagca ctgacgacac
      481 aaatcttatg gggcgtttct tttattaccg ctatcgccat gtggtattac acactgtggt
      541 tgaccatcgc tttctttaaa cgtcggcgtt gtgttcctaa acactatatt atctggctgc
      601 ttatttccgt attgctggcg gtaaaagcgt tcgctttttc acccgtggaa gatggcatag
      661 ccgttcgcca gttgctgttt actttgttgg caaccgcatt gatcgtcccc tatttcaaac
      721 gctcgtcgcg ggtgaaagcg acgtttgtga atccgtaata accttacagt taacctgttg
      781 tcgcctgctc tggattaacg gataataggc ggctttttta tttcaggccg aaaaatgact
      841 gactacctgt tactgtttgt cggaactgta ctggtcaata actttgtact ggtcaagttt
      901 ctcggtctct gtccgtttat gggggtttcc aaaaagctgg aaaccgcgat gggcatgggg
      961 ctggcaacaa cgtttgtgat gacgctggcg tctatttgcg cctggcttat cgatacgtgg
     1021 attttgatcc cacttaatct gatttacctg cgcaccctgg catttattct ggtgattgct
     1081 gtggtcgtgc agttcaccga gatggtggtg cgcaaaacca gcccggtgct ttaccgtttg
     1141 ctggggattt ttttgccgct tatcaccacc aactgtgccg tgctcggcgt ggcgttgctg
     1201 aatatcaatc tcgggcacaa tttcttgcag tcggcgctgt acggtttttc cgccgctgtc
     1261 ggtttttcgc tggtgatggt tctcttcgca gccatccgcg aacgccttgc tgtggctgat
     1321 gtcccggcac cttttcgcgg taatgccatt gcgttaatta ccgcaggtct tatgtctctg
     1381 gcctttatgg gctttagtgg tttggtgaag ttgtaatgaa tgctatctgg attgccgttg
     1441 ccgccgtgag cctgctgggc ctggcgtttg gcgccattct gggttatgcc tcccgccgtt
     1501 ttgcggtgga agacgatccg gtcgttgaga aaattgacga aatcttaccg cagagccagt
     1561 gtggtcagtg cggttatccc ggctgtcgcc cctacgcgga agccatcagc tgtaacggtg
     1621 aaaaaatcaa ccgttgcgcc cctggtggcg aagctgtgat gctaaaaatt gccgagttgc
     1681 ttaatgtcga gccgcagccg ctggatggcg aagcgcaaga gctaacgcct gcgcggatgg
     1741 tggcggttat tgatgaaaat aactgtattg gctgcactaa atgtattcag gcgtgtccgg
     1801 tagacgccat cgttggcgct acccgtgcca tgcatacggt aatgagtgat ctctgtacgg
     1861 gctgcaattt atgtgttgat ccgtgcccga cgcactgcat ctcgttgcaa ccggtcgcag
     1921 aaacacctga ctcctggaaa tgggatctga acaccattcc cgtgcgtatc attcccgtgg
     1981 aacaccatgc ttaagttatt ctctgcattc agaaaaaata aaatctggga tttcaacggc
     2041 ggcatccatc caccggagat gaaaacccag tccaacggta cacccctgcg ccaggtacca
     2101 ctggcgcagc gttttgtcat tccactgaaa cagcatattg gcgctgaagg tgagttgtgc
     2161 gttagcgtcg gcgataaagt attgcgcggc cagccgctta cccgtggtcg cggcaaaatg
     2221 ctgcctgttc acgcgcccac ctcgggtacc gttacggcta ttgcgcccca ctctacggct
     2281 catccttcag ctttagctga attaagcgtg attattgatg ccgatggtga agactgctgg
     2341 atcccacgag acggctgggc cgattatcgc tctcgccgtc gcgaagagtt aatcgagcgc
     2401 attcatcagt ttggtgttgc cgggctgggt ggtgcaggat tcccgacagg cgttaaattg
     2461 cagggcggcg gagataagat tgagacgttg attatcaacg ctgctgagtg cgagccatac
     2521 attaccgccg atgaccgttt gatgcaggat tgcgcggctc aggtcgtaga gggtattcgc
     2581 attcttgcgc atattctgca gccacgcgaa attcttatcg gcattgaaga taacaaaccg
     2641 caggcgattt ccatgctgcg cgcggtgctg gcggactctc acgatatttc tatgcgggtg
     2701 attccaacca aatatccttc tggcggtgct aaacaattaa cctacattct gaccgggaag
     2761 caggttccac atggcgggcg ttcatccgat atcggcgtat taatgcaaaa cgtcggcact
     2821 gcttatgcag tgaaacgtgc cgttattgat ggcgagccga ttaccgagcg tgtcgtaacc
     2881 ctgactggcg aagcaatcgc tcgcccgggc aacgtctggg cacggctggg gacgccagtg
     2941 cgtcatttat tgaatgacgc cggattctgc ccctctgccg atcaaatggt gattatgggc
     3001 ggcccgctaa tgggctttac cttgccatgg ctggatgtcc cggtcgtaaa gattaccaac
     3061 tgtctgttgg ctccctctgc caatgagctt ggcgaaccac aggaagaaca aagctgcatc
     3121 cggtgtagcg cctgtgctga cgcctgccct gcggatcttt tgccgcaaca gttgtactgg
     3181 ttcagcaaag gtcagcaaca cgataaagct accacgcata acattgccga ctgcattgaa
     3241 tgtggggctt gcgcgtgggt ttgcccgagc aatattcccc tggtgcaata tttccgtcag
     3301 gaaaaagctg aaattgcggc tattcgtcag gaagaaaagc gcgccgcaga agccaaagcg
     3361 cgtttcgaag cgcgcctggc tcgtctggag cgcgaaaaag cggctcgcct tgaacgacat
     3421 aagagcgcag ccgttcaacc tgcagccaaa gataaagatg cgattgctgc cgctctggcg
     3481 cgggtgaaag agaaacaggc ccaggctaca cagcctattg tgattaaagc gggcgaacgc
     3541 ccggataaca gtgcaattat tgcagcacgg gaagcccgta aagcgcaagc cagagcgaaa
     3601 caggcagaac tgcagcaaac taacgacgca gcaaccgtta ctgatccacg taaaactgcc
     3661 gttgaagcag ctatcgcccg cgccaaagcg cgcaagctgg aacagcaaca ggcaaatgcg
     3721 gaaccagaac aacaggtcga tccgcgcaaa gctgccgtcg aagccgctat tgcccgtgcc
     3781 aaagcacgca agctggaaca gcaacaggct aatgcggaac cagaacaaca ggtcgatccg
     3841 cgcaaagccg ccgtcgaagc cgctatcgcc cgcgccaaag cgcgcaagct ggaacagcaa
     3901 caggctaatg cggaaccaga agaaccggtc gatccgcgca aagccgccgt cgaagccgct
     3961 attacccgtg ccaaagcgcg caaactggaa cagcaacagg ctaatgcgga gccagaagaa
     4021 caggttgatc cgcgcaaagc tgccgtcgca gcggctattg cacgcgccaa agcacgcaag
     4081 ctggaacagc aacaggctaa tgcagaacca gaagaacagg ttgatccgcg caaagcggca
     4141 gttgccgcgg ctattgcccg cgttcaggcc aaaaaagccg tccagcaaaa ggttgtaaac
     4201 gaggactaaa tggtattcag aatagctagc tccccttata cccataacca gcgccagaca
     4261 tcgcgcatta tgctgttggt gttgctcgca gccgtgccag gaatcgcagc gcaactgtgg
     4321 ttttttggtt ggggtactct cgttcagatc ctgttggcat cggttagtgc tctgttagcc
     4381 gaagctctcg tactcaaact acgcaagcag tcggtagccg ccacgttgaa agataactca
     4441 gcattgctga caggcttatt gctggcggta agtattcccc ccctcgcgcc atggtggatg
     4501 gtcgtgctgg gtacggtgtt tgcggttatc atcgctaaac agttgtatgg cggtctgggg
     4561 caaaacccgt ttaatccggc aatgattggt tatgtggtct tactgatctc cttcccggtg
     4621 cagatgacca gctggttacc gccacatgaa attgcggtca acatccctgg ttttatcgac
     4681 gccatacagg ttattttcag cgggcatacc gccagtggtg gtgatatgaa cacactacgc
     4741 ttaggtattg atggcattag tcaggcgaca ccgctggata catttaaaac ctctgtccgt
     4801 gccggtcatt cggttgaaca gattatgcaa tatccgatct acagcggtat tctggcgggc
     4861 gctggttggc aatgggtaaa tctcgcctgg ctggctggcg gcgtatggtt gctatggcag
     4921 aaagcgattc gctggcatat tcccctcagc ttcttagtaa cgctggcgtt atgcgcaacg
     4981 ttgggctggt tgttctcacc agaaacactg gcagcaccgc aaattcatct gctgtctgga
     5041 gcgaccatgc tcggcgcatt ctttattttg actgacccgg ttaccgcttc tacgaccaat
     5101 cgtggtcgtc ttattttcgg cgcgcttgcg ggcttattag tctggttgat ccgcagtttc
     5161 ggcggctatc ctgacggcgt ggcttttgcc gtcctgctgg cgaacatcac ggttcctctg
     5221 atcgattact acacgcgtcc gcgcgtctac ggccatcgca aagggtaaac catgctgaaa
     5281 actatccgaa aacacggcat tacgttggcg ctatttgcag cgggttcaac agggttaact
     5341 gcggccatca accagatgac caaaacgacg attgctgaac aggccagtct gcaacaaaag
     5401 gctttatttg atcaggtgct gccagccgaa cgctataaca atgcgctggc acagagttgc
     5461 tatctggtaa ctgcgccaga gttaggtaaa ggtgagcatc gggtttacat cgccaaacag
     5521 gatgacaaac cggtagccgc cgttctggaa gcaaccgcgc cagatggcta ttccggtgcg
     5581 attcagctgc tggtgggagc cgattttaac ggcacggtac ttggcacgcg cgtgacagag
     5641 caccacgaaa cgccagggct tggcgataaa atcgaactgc gcctttctga ctggatcacc
     5701 cattttgcgg gtaaaaaaat cagtggtgcg gatgatgcgc actgggcggt gaagaaagat
     5761 ggtggtaatt tcgaccagtt caccggcgcg acgattactc cccgcgcggt ggttaatgcg
     5821 gtaaaacgcg ccggattgta cgctcagacg ttaccggaac aactttctca acttcctgcc
     5881 tgtggagaat aaagcgtgag cgaaattaaa gacgttattg ttcaggggtt gtggaaaaac
     5941 aactctgcgc tggtccagtt gctcggcctt tgtcctctgt tggctgtcac gtctactgcc
     6001 actaacgctc tgggtttagg acttgcgact acgctggtac tgacgctgac caacctgacc
     6061 atttcgacgc tgcgtcactg gacgccagcc gagatccgca ttcccattta cgtgatgatc
     6121 atcgcctcgg tggtcagcgc tgtacagatg ctgatcaacg cctacgcctt tggcctgtat
     6181 caatcattag ggatttttat tccgctgatt gtcactaact gtatcgttgt gggccgcgct
     6241 gaagccttcg ctgccaaaaa aggtccggcg ctttcggcac tggacggctt ttcaattggt
     6301 atgggcgcaa cctgcgccat gttcgtgctg ggttcactac gcgaaattat cggcaatggc
     6361 acattgtttg acggtgcaga tgcgctgtta ggtagctggg caaaagtatt acgcgtggag
     6421 attttccaca ccgactcccc tttcctgctg gcgatgctgc caccaggtgc atttattggc
     6481 ctgggactga tgctggcagg aaaatacctg attgatgaaa gaatgaaaaa gcgccgtact
     6541 gaagcagctg ccgaacgtgc attgccaaac ggtgaaacag ggaatgtctg atgaacaaag
     6601 caaaacgcct ggagatcctc actcgcctgc gtgagaacaa tcctcatccc accactgagc
     6661 ttaatttcag ttcgcctttt gaattgctga ttgccgtact gctttccgct caggcaaccg
     6721 atgtcagtgt taataaggcg acggcgaaac tctacccggt ggcgaatacg cctgcagcga
     6781 tgcttgaact gggcgttgaa ggggtgaaaa cctatatcaa aacgattggg ctttataaca
     6841 gcaaagcaga aaatatcatc aaaacctgcc gtatcttact ggagcagcat aatagcgagg
     6901 ttccggaaga tcgtgctgcg cttgaagccc tgcccggcgt aggtcgtaaa acagccaacg
     6961 tcgtattaaa cactgcattc ggctggccga ctattgctgt cgacacgcac attttccgcg
     7021 tttgtaatcg tactcaattt gcgccgggga aaaacgtcga acaggtagaa gaaaagctac
     7081 tgaaagtggt tccggcagag tttaaagtcg actgccacca ttggttgatc ctgcacgggc
     7141 gttatacctg cattgcccgc aagccccgct gtggctcttg tattattgaa gatctttgtg
     7201 aatacaaaga gaaagttgac atctgaagaa aaggggtaac accgattacc ccattgataa
     7261 cctttcttta tcctctttta aaacatcttt ttaacgtcaa t
//
LOCUS       DAZFKY010000206         7281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFKY010000206 DAZFKY010000000
VERSION     DAZFKY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            299..949
                     /gene="csgD"
                     /locus_tag="ACHKCL_004110"
     CDS             299..949
                     /gene="csgD"
                     /locus_tag="ACHKCL_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5764971.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            954..1343
                     /gene="csgE"
                     /locus_tag="ACHKCL_004111"
     CDS             954..1343
                     /gene="csgE"
                     /locus_tag="ACHKCL_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5764972.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1368..1784
                     /gene="csgF"
                     /locus_tag="ACHKCL_004112"
     CDS             1368..1784
                     /gene="csgF"
                     /locus_tag="ACHKCL_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5764973.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1811..2644
                     /gene="csgG"
                     /locus_tag="ACHKCL_004113"
     CDS             1811..2644
                     /gene="csgG"
                     /locus_tag="ACHKCL_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5764974.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2708..3199)
                     /gene="ycdZ"
                     /locus_tag="ACHKCL_004114"
     CDS             complement(2708..3199)
                     /gene="ycdZ"
                     /locus_tag="ACHKCL_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5764975.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3301..3855)
                     /gene="ycdY"
                     /locus_tag="ACHKCL_004115"
     CDS             complement(3301..3855)
                     /gene="ycdY"
                     /locus_tag="ACHKCL_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5764976.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3879..4616)
                     /gene="ycdX"
                     /locus_tag="ACHKCL_004116"
     CDS             complement(3879..4616)
                     /gene="ycdX"
                     /locus_tag="ACHKCL_004116"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5764977.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4671..5609)
                     /gene="ghrA"
                     /locus_tag="ACHKCL_004117"
     CDS             complement(4671..5609)
                     /gene="ghrA"
                     /locus_tag="ACHKCL_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5764978.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5843..5930
                     /locus_tag="ACHKCL_004118"
     tRNA            5843..5930
                     /locus_tag="ACHKCL_004118"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5877..5879,aa:Ser,seq:gga)
     gene            complement(6080..6922)
                     /locus_tag="ACHKCL_004119"
     CDS             complement(6080..6922)
                     /locus_tag="ACHKCL_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5764979.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(7007..7204)
                     /locus_tag="ACHKCL_004120"
     CDS             complement(7007..7204)
                     /locus_tag="ACHKCL_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5764980.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7216..>7281)
                     /locus_tag="ACHKCL_004121"
     CDS             complement(7216..>7281)
                     /locus_tag="ACHKCL_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5764981.1"
                     /translation="VSLIHLDASAECLPGLPTFDW"
BASE COUNT         1934 a         1782 c         1665 g         1900 t
ORIGIN      
        1 ttttaaaatt gtgcaataaa aaccaaatgt acaacttttc tatcatttct aaacttaata
       61 aaaccttaag gttaacattt taatataacc agttacactt agttacatgt ttaacacttg
      121 atttaagatt tgtaatggct agattgaaat cagatgtaat ccattagttt tatattttac
      181 ccatttaggg ctgatttatt actacacaca gcagtgcaac atctgtcagt acttctggtg
      241 cttctatttt agaggcagct gtcaggtgtg cgatcaataa aaaaagcggg gtttcatcat
      301 gtttaatgaa gtccatagta ttcatggtca tacattattg ttgatcacta aaccttcttt
      361 gcaggcgaca gctctcttgc agcaccttaa acaatcgctg gcaattacag gaaaattaca
      421 taatattcaa cgttctctgg acgatatctc ttcaggctct attattcttc tggatatgat
      481 ggaagcggat aaaaagctta tccattattg gcaggatact ttgagcagga aaaacaacaa
      541 tatcaaaata ttgttgctaa atacgcctga agattacccg taccgcgaca ttgaaaactg
      601 gcctcatatc aacggcgttt tttatgccat ggaggatcaa gaacgcgttg tcaatggatt
      661 gcaaggcgtc ctgcgcggcg aatgctactt tacgcaaaag cttgccagtt acctgattac
      721 acattctggt aactatcgtt ataacagcac ggaatcagcc ctccttactc atcgggaaaa
      781 agagatcctg aataagctgc gtatcggcgc gtctaataac gagatcgctc gttcgttgtt
      841 catcagcgaa aatacggtta aaacgcatct ttataatctt ttcaagaaga tagccgtaaa
      901 aaaccggaca caagcggttt cctgggcaaa cgataacctc aggcgataaa gccatgaaac
      961 gttatttacg ctggattgtg gcggcagaat ttctgttcgc cgcagggaat cttcacgccg
     1021 ttgaggtaga agtcccggga ttgttaactg accatactgt ttcatctatt ggccatgatt
     1081 tttaccgagc ctttagtgat aaatgggaaa gtgactatac gggtaactta acgattaatg
     1141 aaaggcccag tgcacgatgg ggaagctgga tcacaataac ggtcaatcag gacgttattt
     1201 tccagacttt tttattcccg ttgaaaagag acttcgaaaa aactgtcgtc tttgcactga
     1261 ttcaaactga agaagcacta aatcgtcgcc agataaatca ggcgttatta agtacgggcg
     1321 atttggcgca tgatgaattc taaataaaaa attgttcgga ggctgcaatg cgtgtcaaac
     1381 atgcagtagt tctactcatg cttatttcgc cattaagttg ggctggaacc atgactttcc
     1441 agttccgtaa tccaaacttt ggtggtaacc caaataatgg cgctttttta ttaaatagcg
     1501 ctcaggccca aaactcttat aaagatccga gctataacga tgactttggt attgaaacac
     1561 cctcagcgtt agataacttt actcaggcca tccagtcaca aattttaggt gggctactgt
     1621 cgaatattaa taccggtaaa ccgggccgca tggtgaccaa cgattatatt gttgatattg
     1681 ctaaccgcga tggtcaattg cagttgaacg tgacagatcg taaaaccgga caaacctcga
     1741 ccatccaggt ttcgggttta caaaataact caaccgattt ttaagcccca gcttcataag
     1801 gaaaataatc atgcagcgct tatttctttt ggttgccgtc atgttactga gcggatgctt
     1861 aaccgccccg cctaaagaag ccgccagacc gacattaatg cctcgtgctc agagctacaa
     1921 agatttgacc catctgccag cgccgacggg taagatcttt gtttcggtat acaacattca
     1981 ggacgaaacc gggcaattta aaccctaccc ggcaagtaac ttctccactg ctgttccgca
     2041 aagcgccacg gcgatgctgg tcacggcatt gaaagattct cgctggttta taccgctgga
     2101 gcgccagggc ttacaaaacc tgcttaatga gcgcaagatt attcgtgcgg cacaagaaaa
     2161 cggcacggtt gccattaata accgaatccc gctgcaatct ttaactgcgg caaatatcat
     2221 ggttgaaggt tcgattatcg gttatgaaag caatgtcaaa tctggcgggg ttggggcaag
     2281 atattttggg atcggtgccg acacgcaata ccagctcgat cagattgccg tgaacctgcg
     2341 cgtcgtcaat gtgagtaccg gtgagatcct ctcctcggtg aacaccagta agacgatact
     2401 ttcctatgaa gttcaggccg gggttttccg ctttattgac taccagcgct tgctggaagg
     2461 ggaagtgggt tacacctcga acgaacccgt tatgctgtgc ctgatgtcgg ctatcgaaac
     2521 aggggtcatt ttcctgatta atgatggtat tgaccgtggt ctgtgggatt tgcagaacaa
     2581 agcagaacgg cagaatgaca ttctggtgaa ataccgccat atgtcggttc caccggaatc
     2641 ctgacagata aaaatgccgg aattaataat ccggcattta ctgtttaagc gaaagcgtga
     2701 gtgcccctca cgctttattt ttgatttcct gctcacggag agccgtcttt gcactacgcg
     2761 ccgccagcca caggccgctg tttttcattg cgtaaccaaa tatcagccca agcgccagcg
     2821 aaggtaacac cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg cctataaacg
     2881 tccccggaac aaatgaaagc agcagctgtt tggcctgaat acacatcaga aacgcgacaa
     2941 taccggttat gacataactg agaatttcca gatgtggcgc cagtgcacta ccgtaaataa
     3001 tgaccatcgc ccacaccacg ccactaagca gagttgcagc ggagatcgcc agccctttca
     3061 gcccaccttg cgggcaggca aagtaggccg tacagcccag gaagcccgcc cagcttagta
     3121 agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt gtgattgcaa
     3181 tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg aatgagatcg
     3241 agcacacatt ttatgcaaca gcaaattatc tgttgcagtt atgagtgacg catatcacat
     3301 ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatggcat cgcgggttag
     3361 cggtgccatg gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc caaggaacgc
     3421 accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc tctcatcttc
     3481 cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt gatcggcagg
     3541 cgtatccgct aatggcatcc ctcgttcaga aagaaaagcg cgcacttccg cttccgttgc
     3601 gtcctcaacc catgcgctac gatatggcgg cacggcacat tcatcgccga taaacaacgc
     3661 attgtaatcg gcagagactt gcgtcatatc acagcttttc tgcaaacgtg tcagcaactc
     3721 atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcaggg taaacagcgg
     3781 caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac gacagaggat
     3841 agaaaactcg ttcattaata tatttccatt actagtaatt aaaggtctgc aaattccgca
     3901 atcggtgcca taccgcgaga ttcaaggaag ttcagtaagc ggcgcggaga aacattcaaa
     3961 atgcgctctg gcggaaaatc taccgcgtcg aggattttaa gacactcttc aaattccccc
     4021 atggtaaacg cggtgtgaga atccgagcct aatgccaccc aacctccagc atcacgtacc
     4081 gctgcggcta ccgcacgaca gttgtcttca ctgcccttac gtgagtgtaa aaatgaggaa
     4141 ttattgattt ccagcgccac ctgatgtttc gcggctgctt cagcaacggc tttaacatct
     4201 atctcatatt tcggatttcc gggatggctt attatatgca cattgccgct ggcgatagtg
     4261 gcgatcatcg cttgtgtatt ggtcgctttg tcatgtggtg caaaaaccgg ctcatgaaaa
     4321 ccggcaataa tcagatccag cgagtcaaac attttaccgc tgcaatcaat ttcaccatca
     4381 acatttttaa tgttagcttc gatgccgcgc aggatcccta ccccatcaac cactcgcggc
     4441 cagatacgca tgttaatgaa gtgccagtga tgcggcgcat cttccatgtc cgggccatga
     4501 tcggtaatcg caaaaagttt aatgcccttt tgtttggcct gggcaatgta atcactcaat
     4561 gtgctatatg catgtgtgct ggcaacggta tgcatatgaa ggtcgacggg atacataact
     4621 ctctcctgta ttcaattccc gccaaggata gcaggaatcc tgatgcttta ttagtagccg
     4681 cgtgcgcggt cgacttgccc gcagaccctc tccccttttt cgagctgggc aatagtgcga
     4741 gaaatgtact ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg tgttatcgtc
     4801 acgcgtggat gttgccagag cggactttca ggcggtaagg gttcacgatt aaaaacatcc
     4861 aacattgcgc ctttaacttt gccgctatcc agcgccgcga gcaggtcatc ttccacgaca
     4921 tgaacaccac gcgccaggtt gaggagatac gcgccatccg gtaatttttc gagtaattgt
     4981 tgattaataa tgccgacggt ttcaggggta ttcggtaaca aattaatcaa tacccgacat
     5041 tggctcagaa atgcagacag ttcttcccat ccggcaaagc tttgcacgcc aggccacgat
     5101 ttacgggttc gactccagca acgcagcgga aagcgccagg tttgcagact ctgagcaact
     5161 ttactgccca gtacgcctgc gcccaaaatg ccgatggtaa aatcttcccg atgatattca
     5221 ggcagcggtt gccaatgcga actattttgc tggatgcgat aatcgtcaaa acgtcgaaac
     5281 caatgcagca cctgactgac agcatattcc tgcatttgct cgcccatacc ggtatcttcc
     5341 aggcgaaaaa gtggaacaga aggcttcagc atttcagggt gtgcctgtag cttgctcaaa
     5401 atagaatcaa caccggcccc gagtgcgaac accgctttaa gatcgcgccc tgccagcatt
     5461 tcaacaggag gatgccaggc taaagcataa tcagcagagt cattatctcc gcttttccat
     5521 gctctgactc ttgcctgagg aatagctttg cgcagtgcct ctatccacca ttgggtatcg
     5581 aacgttgggt gataaaagat gatatccata ctgactcccg aaaagcgttg tgcgaaattt
     5641 attcgcactt atcgttatga tctacaaaga ccaccagcat aacaaatccg tggtcggtgg
     5701 ccaaaaaagc agattttgct tattaaaact gcatattgat tgaagtttga ataaacgcgc
     5761 gattttttaa aaaagtttgt tgacctcagg tcatgatttc cctaaattag cgcccgttcc
     5821 agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa
     5881 agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca gattataagc
     5941 gcatgttatt gtttaacatg cgctttttta ttttcccttc cagacagtat gccatcgggt
     6001 atgccatctg aaaccggttt atatgccccg cgatcatatc ttatcggaca atttccgatc
     6061 tttgagcata ctggcaaagc tatactgcca gcatatcagg atagtgtttt gcgataatat
     6121 cattcagtct gtcaaccagt tctggttttc tgaacgtgat atgcgcggag cccttcttaa
     6181 agtatctgat gctgaacatc tcatcttcat agctctcttt accctgaaca gactgaatgt
     6241 gatcatccag acgaagggtg atgtttcccc ggttgtcggg gattggttta ccgctgaaca
     6301 ggtgcaacat tctttccaga tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa
     6361 atccccaccg gtcccatctc accagattgt tgacgataat tttactgcca aatttacagg
     6421 ggcaattggt tttgtaattc cagttcagcc ctctgaagac gttgatcacc cctcgctcaa
     6481 acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc gcttcgctga
     6541 tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggcc tgcgtatcca
     6601 ttaatgacag catcccggaa cgttgcatca ggtcacgcca gatttcgcga tcaagattcc
     6661 gggtaatggc tttcattgct gtttcgggtt tctccatcag ccagcagccg cagcgaaaat
     6721 cctgtttcat ggcccagtcc ggggcagttt tacctccgat gctttccgtc agcaccgaga
     6781 tttcagccag ttgtcggata agtgtgtcga tttgcaacag tgtactcttt cgtacagcca
     6841 gaatgtggcg aatgctggtt gagcagataa cgtccgtatg cccgttgacc acttccggac
     6901 gggagattgt gatatctgac ataatcgata ttcctttttg cagataaatg aaaacgctag
     6961 ccctgtgcag gctggcgtca gaggaatgaa gtctggtgag tgagcttcac cggttctggt
     7021 gcagaagctg cagatgacgc agggtacgga catcctcccg ggcctgttcg atacagggca
     7081 acagtgctgc tgaatccgtt ttatcctcat cgttgtcaaa gataattccc gatccgcagt
     7141 cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt
     7201 tcatgttgtt cctggttacc agtcgaaagt gggtaaaccc ggcaggcatt cagcgctggc
     7261 atccagatga atgagactga c
//
LOCUS       DAZFKY010000207         7247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFKY010000207 DAZFKY010000000
VERSION     DAZFKY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            71..1404
                     /gene="adeQ"
                     /locus_tag="ACHKCL_004122"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             71..1404
                     /gene="adeQ"
                     /locus_tag="ACHKCL_004122"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1457..1909
                     /gene="yicN"
                     /locus_tag="ACHKCL_004123"
     CDS             1457..1909
                     /gene="yicN"
                     /locus_tag="ACHKCL_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5764982.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2025..2348)
                     /locus_tag="ACHKCL_004124"
     CDS             complement(2025..2348)
                     /locus_tag="ACHKCL_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5764983.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2332..2685)
                     /locus_tag="ACHKCL_004125"
     CDS             complement(2332..2685)
                     /locus_tag="ACHKCL_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5764984.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2919..4109
                     /gene="nepI"
                     /locus_tag="ACHKCL_004126"
     CDS             2919..4109
                     /gene="nepI"
                     /locus_tag="ACHKCL_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5764985.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4150..4443)
                     /gene="yicS"
                     /locus_tag="ACHKCL_004127"
     CDS             complement(4150..4443)
                     /gene="yicS"
                     /locus_tag="ACHKCL_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5764986.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4665..5483
                     /gene="nlpA"
                     /locus_tag="ACHKCL_004128"
     CDS             4665..5483
                     /gene="nlpA"
                     /locus_tag="ACHKCL_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5764987.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5487..6410)
                     /gene="yicL"
                     /locus_tag="ACHKCL_004129"
     CDS             complement(5487..6410)
                     /gene="yicL"
                     /locus_tag="ACHKCL_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5764988.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6521..6694)
                     /locus_tag="ACHKCL_004130"
                     /pseudo
     CDS             complement(6521..6694)
                     /locus_tag="ACHKCL_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6874..6979
                     /locus_tag="ACHKCL_004131"
                     /pseudo
     CDS             6874..6979
                     /locus_tag="ACHKCL_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            6990..7055
                     /locus_tag="ACHKCL_004132"
                     /pseudo
     CDS             6990..7055
                     /locus_tag="ACHKCL_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1773 a         1609 c         1809 g         2056 t
ORIGIN      
        1 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
       61 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc
      121 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
      181 gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc
      241 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
      301 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
      361 cttctttgcg ttcgtggtcg tgggggcgat gggtatctcc tggcagaccg ggatgggcgc
      421 gatattctgg ggcgcaattg ggctattttt gctcacgcta tttcgtatcc ggtactggat
      481 gatctccaac attccattaa gtttacgtat tggtatcacc agcggaatcg gattatttat
      541 cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca ctctggtgat
      601 gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac
      661 cgtgttgtca tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg tgacgtcttg
      721 ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat
      781 tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat
      841 cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac
      901 tgataaagct ggcttaatag atagtaacgg taaattcccc aatatgaata aggcgctata
      961 tgttgatagc gtagttcggt ggcgggcgcg tttatcggca cctcgtctgt taccgcctat
     1021 attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg ggctgactgc ggttgtggtc
     1081 ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg tggcgatggt tcctccttac
     1141 gcaaccgccg gagcgttaat ctttgttggc gtgttgatga cctcaagtct ggcgcgcgtt
     1201 aactgggatg attttaccga atcggtgcct gcgtttgtta ccacggtgat gatgcccttt
     1261 actttctcga tcaccgaagg gattgcactt ggctttatgt cgtactgcat catgaaagtg
     1321 tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca
     1381 ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga
     1441 ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt
     1501 tggttttcgg gtgctgacat ccggggccag aaaagcgatt cgccgtctca gcgatcggct
     1561 gaatatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga
     1621 cgaattttta cgttatttgc atcgtccgga tgagtcgcac ctgcaaaacg ccgcgcaggt
     1681 gttgctcatc tggcaaattg tcattgtcga tggtagcgaa cagaacctgc tgcaatggca
     1741 tcggatttta caaaaagccc gccttgccgc tcccattact gatgctcagg tcaggctggc
     1801 gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg
     1861 ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt
     1921 cttatcagcc tacagaccgc gcctgacctg tagggtggat aaggcgttca cgccgcatcc
     1981 ggtatcggaa ctgacagtag gtgaacgcat tatctacgtt ctatttacag cagcacctcc
     2041 aacccgtgtt tttgcacaat gctgagcagt tttaatgaaa tgccattggg gcgtttagcg
     2101 ccactttccc atttttgtac agttgaaacg ctggtattga gatagtgagc gaagacgggc
     2161 tgactcactt ttaatttttc ccgcagtgct tttatctcaa caggttgtaa gtcttgcacg
     2221 ctggtcaggc aggctttgtc aaaagtacgc atcgtttcag cagaaatagc attgacgctg
     2281 gacaatccag ccgctgcgct gtgaatagcc tcaaatgcag gacttttaaa tttagttttt
     2341 gcagtcatag cagatctctg tcagttcttt agtgttaatc attgctgtta gttgggcttt
     2401 cgtcaggaag gcataatgct ttgcaatttc acgaaaccca gccagttcct gactattgat
     2461 gttcgacata tcctgttttg catataaaaa cgtgtaaaac caattgctta cccctttcgc
     2521 cagaataatt gcacgctcac gattttggtg aaggcgcttt ttaaaaacgc caccgccaag
     2581 tgcatcagct ttaccttgtt ctgtttctaa aatggcccgg cacaactctt catctgttat
     2641 tgcatgagac ctggcttccc ttgcaaacca tttggtttta aacacgcgta tatcgttgag
     2701 gtcctgttat taaagtatat caccaagtgc aatactttat cccttgttcc caacacaatc
     2761 gcgaaaatac atatcacctt gtcctatgat ttttatttct tcgagcagcc tcgagctttt
     2821 cccacaacac cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca atgttaaagt
     2881 ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt attgccgaaa
     2941 accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg gtggcgtttt
     3001 gcgtcgcctg tctgattatc gttgagtttt tgcctgtcag tttgttgacg ccaatggccc
     3061 aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg
     3121 caatgtttgc cagtttgttt attacccaga caattcaggc cactgaccgc cgctacgttg
     3181 ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt gctaactcat
     3241 tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg ttctgggcga
     3301 tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag gcgctgtcgg
     3361 tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc tgttttttag
     3421 gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggcgatgggt gtgctgtgta
     3481 ttttctggat tatcaaatca ctgccttcgc tgccaggtga accttcgcat cagaaacaaa
     3541 atactttccg cttattacaa cgtccgggag tgatggccgg gatgatcgcc atctttatgt
     3601 cctttgccgg acagtttgct tttttcactt atattcgccc agtgtatatg actctggcgg
     3661 gattcggtgt tgatggctta accctggtgc tgctgagttt tggtatcgcc agctttgtcg
     3721 ggacatcgct ttcgtcgttt attctcaaac gttcggtgaa actggcctta gcgggcgcac
     3781 cgtttgtgct cgccctgagc gcgctggtac tgacgctgtg gggaagcgat aaaattgttg
     3841 ctactggtgt ggcgattatc tgggggctga cctttgcttt aattcccgtt ggttggtcaa
     3901 cgtggatcac tcgctcattg gccgatcagg cagaaaaggc tggatctatt caggttgccg
     3961 ttattcagct tgccaatacc tgcggtgcgg caatcggcgg ttatgcgttg gataacattg
     4021 gtctgacttc gccgctgatg ttgtccggca cattgatgtt gctgactgca ttgttggtga
     4081 ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg tcagtggttg
     4141 ttgccgttct tacatatccg ggcattctac cttacccaac gcttcccaat aattgctctg
     4201 attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt tttcatctga
     4261 caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc aaattttacg
     4321 taaatcttgc aataccgtgg ttttctcttt tgccgactgt agagaactga atggcgactc
     4381 ggcctgagaa agggctggaa aaatcaggaa tacggtaatc atgagtagca tcgttggctt
     4441 catagcggca accttctgca aatgtgcatc gaatgaagca taagtgtagt ttgcttttgc
     4501 cgctctcatc atgctgtacg aaaggtgttt gcgtggtcag taagaagtgc cagactttat
     4561 attccacttt tattcctttt tattcttatc gatagcgttt tgttttttaa actgcagcga
     4621 ccttaccgct atagtccggt aatcattaat aaaaggataa aaaaatgaaa ctgacaacac
     4681 atcatctacg ggcgggggcc gcattattac tggcaggagt tctgctggca ggttgcgacc
     4741 agagcagcag cgatgaaaag catattaaag ttggcgttat taatggcgca gaacaagatg
     4801 tcgcagaagt cgctaaaaag gtagcaaaag agaaatatgg cctcgatgtt gaactggtag
     4861 gatttagcgg ttcgttgcta ccgaacgatg cgacaaataa tggcgagctt gatgcgaacg
     4921 tcttccagca tcgacccttt cttgaacaag ataatcagac gcatggttat aaactggtag
     4981 cggtgggaaa tacgtttgtc ttccctatgg cgggctattc caaaaaaatc aaaaccgtgg
     5041 cgcaaataaa agaaggtgcg acagtggcga tccccaacga cccgactaac cttggtcgcg
     5101 cgcttttact gctgcaaaaa gagaagctaa ttactctgaa agagggaaaa gggttattac
     5161 ctaccgcgtt ggatataacc gataatccac gccatttgca gataatggag ctggaagggg
     5221 cacagttgcc acgtgtactg gacgacccga aagtggatgt agcgattatc agcaccactt
     5281 acatcgagca aacaggactt tctccggtta acgacagcgt atttattgaa gataaaaatt
     5341 cgccgtatgt gaatattctg gtggcacggg aagataataa gaatgcagaa aacgtgaagg
     5401 aatttctgca atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc atctttaacg
     5461 gtggtgcggt gcctggctgg taattctcac tcatgccgcg ccggacgatt tatttttctg
     5521 gcgcggcggc gggaatccat tgaaatcaaa atcaccgatg acagaatcag cagcgttccc
     5581 agccagtccg gcaatgtgaa ggtgatcccc agcaacagca aagagagcag cgcgctactt
     5641 aatggttcag cacagcttaa aatgctggct tttggaccgc caattaattg tgctcctttc
     5701 aggtacagac taaatgtcag ggacgtacca atgaccacca aataaaaaaa cgccagaatc
     5761 aaacttccgt taacgataaa gtttgtccct tgtccggcat aaaagggcag caggatcaga
     5821 ccgccaatga gcatactcca gccgacgacc ggtaacgtgc catagcgggc aatcagcgtt
     5881 gagggatagg tggtatagaa tgcagcagta aatgccgagg caatgcccca gaataacgcg
     5941 gcaggagaga tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa agtcccgatc
     6001 agcgatgtca aaatagcgca gaaaaccaga atgcccgggc gggatttacg caccagtgag
     6061 aaccaggcga cgataatagt cggtgagagg aattgcagca ccgttgccgt ggctgcgttc
     6121 gatttttcga tggttagcaa aaaagtgagc tgtacggtta gcgcgccaac cacggaaaaa
     6181 atcagtaggc taatggcatc tttgtgattg ttaataatag aaaagatttt atcaccatga
     6241 acaaatgata gcgtcagcag aatcaaaccg gcgaatatca aacgcgtcat agtcaaaaac
     6301 tgcgacgaca tctggctttg ctccatgatg tattgcgcgc agacccctga acttccccac
     6361 aacacggcgg caatcagaac gttcagcatc ccctttctgg tggaacccat ttttccctct
     6421 gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga ggatggctca
     6481 gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct ttaataaaca
     6541 gcaagcaaat cattgcaata cccaacatcc ctatcgccag ccagaacaac gtgtggtagc
     6601 tccagatttc aaccataatt tcgtcaacag agccggcgat aatctagcct aagcgtgagg
     6661 tatttgcata taacgttgtt gcggaaccta ttcttattta tcggagtgcc ttgccagtaa
     6721 tttactgacg aggatcctca taatagcgtt acccatctgt tgcattgaga ctttacaggc
     6781 cgttattcta aatgaccaaa attaaatcag tgcataaggg gaagtattct gtagtgatgt
     6841 ctccctgaga tgcaggttcc actggagaaa attatgagca gtttgtttca gaatgaaata
     6901 aaggataaat ttaaaactgg acttgcatat gtgcgggggc cagaaaaaag cggatcttcc
     6961 gctcaaaatg caggtagcga aggtggtaaa aaatgcgccg ggagaagcag aagattgtcc
     7021 cgcgatgtcc gtttgagaaa cacgagtttg attaaacgcg tgagcatgat gatattagta
     7081 gctgaccaga gaaattatat ccgtcgtaag caataaaaat cagaagtgat gttatggata
     7141 tttttaacct gaataactcc gtaagtacgg ggttattcag gtttttgcta gcctgtgctt
     7201 ttctatacca gatgtggtgt gaacactcta ttctgcatac aaattaa
//
LOCUS       DAZFKY010000208         7215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFKY010000208 DAZFKY010000000
VERSION     DAZFKY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..749
                     /locus_tag="ACHKCL_004133"
     CDS             <1..749
                     /locus_tag="ACHKCL_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5764989.1"
                     /translation="DAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVDSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSNGAGMESPWNAVAGVNWSF"
     gene            complement(1121..1387)
                     /gene="minE"
                     /locus_tag="ACHKCL_004134"
     CDS             complement(1121..1387)
                     /gene="minE"
                     /locus_tag="ACHKCL_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5764990.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1391..2203)
                     /gene="minD"
                     /locus_tag="ACHKCL_004135"
     CDS             complement(1391..2203)
                     /gene="minD"
                     /locus_tag="ACHKCL_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5764991.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2227..2922)
                     /gene="minC"
                     /locus_tag="ACHKCL_004136"
     CDS             complement(2227..2922)
                     /gene="minC"
                     /locus_tag="ACHKCL_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5764992.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3442..3810
                     /locus_tag="ACHKCL_004137"
     CDS             3442..3810
                     /locus_tag="ACHKCL_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5764993.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3913..4314)
                     /gene="pliG"
                     /locus_tag="ACHKCL_004138"
     CDS             complement(3913..4314)
                     /gene="pliG"
                     /locus_tag="ACHKCL_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5764994.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4385..4543
                     /locus_tag="ACHKCL_004139"
     CDS             4385..4543
                     /locus_tag="ACHKCL_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5764995.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4555..4848
                     /locus_tag="ACHKCL_004140"
     CDS             4555..4848
                     /locus_tag="ACHKCL_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5764996.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4920..5579
                     /locus_tag="ACHKCL_004141"
     CDS             4920..5579
                     /locus_tag="ACHKCL_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5764997.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5671..6117
                     /locus_tag="ACHKCL_004142"
     CDS             5671..6117
                     /locus_tag="ACHKCL_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5764998.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6324..>7215)
                     /gene="hlyE"
                     /locus_tag="ACHKCL_004143"
     CDS             complement(6324..>7215)
                     /gene="hlyE"
                     /locus_tag="ACHKCL_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO5764999.1"
                     /translation="KTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFK
                     QEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKA
                     SAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQ
                     SQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTL
                     SNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAANKMIN
                     TCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1848 a         1593 c         1746 g         2028 t
ORIGIN      
        1 ctgatgcggg tgccgggttt gagcagacgc tgaccggaat gacagtgggg atcgacagcc
       61 gtaatgatat tcctgagggg attgcgacgc tgggcgcttt tatgggttat tcccattcac
      121 atatcggttt tgatcgtgga ggacatggca gtgtggacag ttattctctg ggcggctatg
      181 ccagttggga acatgaaagt ggtttctatc tggacggtgt cgtgaagctg aaccgttttg
      241 aaagtaacgt agccggtaaa atgagcagcg gtggagccgc caatggcagt taccatagca
      301 acgggctggg cggtcacatt gaaaccggga tgcgatttac cgatggtaac tggaacctga
      361 cgccgtatgc ctcgttaacg gggttcaccg ctgataaccc cgaatatcat ttatccaatg
      421 gcatggaatc gaaatcagtc gatacccgca gtatatatcg tgaactgggt gcaacgctga
      481 gttacaacat gcgtctgggg aacggtatgg aagttgagcc gtggctgaag gcggctgtgc
      541 gcaaagaatt tgtcgatgat aaccgggtga aagtgaatag tgacggtaat ttcgtcaatg
      601 atttgtcggg cagacgtgga atataccagg caggtattaa agcctcattc agcagtacgt
      661 taagcgggca tcttggggtg gggtatagca acggtgctgg tatggaatcc ccgtggaacg
      721 cggtggctgg tgtgaactgg tcgttctgac catcaacgat taaactgcgc ttcggcgcag
      781 ttttcgttta caggatgtta aaagagaaaa ttctggggta aaaaaagccc gccagttacg
      841 gcgggaaacc tcatcctatg ggagaacaat gaataatgaa attgcggggt tatcatctcc
      901 cagtatatca acactaacaa taaggttatt tactcaacca ggcataaata ttttgttttg
      961 tgcgtgggaa cagccttaag gtgtaaaggg ggaggtggaa atagcaatga ggagtatcag
     1021 caagaatact cgccgcttta ccacaacgtg gatgagaggg atgaaaaact caaggcagag
     1081 ataactctgc cttgaagata aatgcgcttt tacagcggga ttatttcagc tcttctgctt
     1141 ccggtaaggt cacgttcagc tcaagaatag aaatatcgcc atctttttgc tcaagctgta
     1201 cggttaccat ctcaggatca atttgtacgt atttacaaat gacctcaaga atatctttac
     1261 gcaactgcgg cagataatgc ggttctgcat cgctgcgacg gcgttcagca acaataatct
     1321 gcagccgttc ttttgcaatg ttggctgtat ttttcttccg cgagagaaag aaatcgagta
     1381 atgccataac ttatcctccg aacaagcgtt tgaggaagcc tttcttctct tcttcaatga
     1441 agcggaaagg acgttcttct cccaacagac gttctacggt atctgcgtag gctttacccg
     1501 catcggcgtt aatgtcgaga atgaccggtt caccctggtt agaggcgcgc aatactgatt
     1561 gatcctctgg gatcacgccg acgagtttga tgcgcaggat ctccagcaca tcttccatgc
     1621 tcagcatgtc acctctgctt acgcggcctg ggttatagcg cgttaacagc aggtgctctt
     1681 taataggctc ttcgccattt tctgcgcggc gtgattttga cgccagaatg cctaaaatac
     1741 ggtcagagtc gcgtactgag gagacttccg ggttggtggt aataatggct tcgtctgcaa
     1801 aatagagtgc cattaacgca ccggtttcaa tccctgccgg ggagtcacaa acgataaatt
     1861 caaaatccat cgctttcaga tcatcaagaa ctttagcgac cccttcacgg gtgagggcat
     1921 ctttatcgcg tgtttgcgat gccggcagaa tatagagatt ttcagtacgc ttatctttaa
     1981 ttaacgcctg atttagcgtt gcatcgccct gaatgacgtt gacgaaatcg taaacgaccc
     2041 ggcgttcaca acccataatc aggtcgagat tacgcaggcc gatatcaaaa tctatcacga
     2101 cagttttctt tcccttctgg gccaaaccag tggcgatggc cgcgctggag gttgtcttac
     2161 caacaccccc tttgcccgaa gtaacaacaa taatgcgtgc catagaaatt ccttgttaaa
     2221 aagggatcaa tttaacggtt gaacggtcaa agcgttttcg actaactgca gtcgcgccgc
     2281 tttgccataa aattctgctg ggatttgatc actcagccag tattcacctg cgatggacac
     2341 caattccgcc atcaggttcg tacaaaatat ttgcgtttcc cggtcaccac ttgcccctgc
     2401 cagcgcacga ccgcgcatca tgccatagac atgaatgttc ccatcggcaa tcaattcggc
     2461 cccagcgcta acgtggcttg taacaatcag atcacattgt ggagcataaa tacgctgacc
     2521 ggaacgcacc ggggtatcta ttaaacgcgt ttttgtgacc ggcgttgtat tttgcgctgg
     2581 agcctgcggt gcgggagctg gacgtggcgc tttttccttt ccttccgtca ggataggcag
     2641 ccccattttt tcaatttcgg ctttaagttg cgcatctttg cagccactta cgccaataac
     2701 ccgcaaaccg gttgccgaaa ccgccttatg catcgctgac cagtttaccg ggtcttccag
     2761 tgcactgacg ttgagtacaa cgggggcatg ttttaaaaat gcgggggcct gagcgatttt
     2821 gtcttccagc gcctgatgga taaccttagg ttctgcctca tgcagatgaa ccacagataa
     2881 agtgaagcta ctgcctttaa gctcgattgg cgtgtttgac atcctggcct tactcaatta
     2941 gctattaatc atcgccagcg cgcgatgatg ttccgaagac tataaggcat gttatagtct
     3001 ggattatatt gaggcaagtc accctcccat ttattcagag taaaagtcta ttctgtgata
     3061 aatggcgctg attcatagct taaaaaatac ccttgtcaat caacccattg ccgtcgtact
     3121 tttgattctt cttatttacg cttccttttt ccgcacccta atcgtgacag catctccaga
     3181 cctgtttaaa tatccagtat tgaggcaagt gtattttttc accacgccag gctttcttaa
     3241 ctttccattt tttgcagcat cattaaagaa cagatacccg ttatcttaat atccgccaca
     3301 gaattgctct atcatagaaa ccgcagaaga cgtctgatac ttctgtcagg tcatcgtaac
     3361 tcgtccaggt cagcggtttt gattacggat tgatgatgaa atgagcataa gtgttgtacg
     3421 gttggattat caggatgtgc tatgaatatg atgcgtattt tttatatcgg attgtcaggt
     3481 gtggggatga tgttctcatc aatggcgtct ggacatgacg ctggcggact tcaatctccg
     3541 gcgtgcggcg ttgtttgcga cccgtatatt tgtgtgaact cagatggcat ttccccagag
     3601 ttaacaagga aatatctcag cgaaaaagcc gctgaaaact tacaatcatt acaaggctac
     3661 gatcccagcg aatttacatt cgctaacggt gtattttgcg atgttaaaga aaaattatgc
     3721 cgtgatgatc gttattttgg tgtagatggt aagcggagtg gaaaaatcaa tcaaaccacc
     3781 acaaagatgt tatttatgtg tcgtgagtga tgtcttgttt acattatgtt ctattcgtca
     3841 ttaacctctt gagcgacaga gaggagtggg gcgtcaacgt ttatgacggg gagactcccc
     3901 gtcggttgac atttacttaa tttgaatatc gacgttatat tttttagttt tgtttttacg
     3961 ggcgtcgttt cgggtttgca gcactcgcag ctcatatttg ccagaagcag ggagtgagta
     4021 ttgtccgtga ctatcgagtt caggggaata tctggataaa tcaacagaat catcgattcc
     4081 tggcccgaag aggtaagtat cagcgccttc attagagata ctcacatgca ctttctgacc
     4141 ttttttggca tagaaggtat atgtatcgta atcgtatccc tttatttcgc cggagtactg
     4201 agcgctgcta tggccttttc tgaactcaac attgacattc ttacccgcag caaagctcgt
     4261 ggaggttagc agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag
     4321 tcttctgttc agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat
     4381 tccagtggct ttcctgcgtt cggtaaaatc ccgtcgcggc atttttgccg gggggattac
     4441 ttataaaggg gcatggactc aacaaaccgt agaaatttac agctggctgg cttgtggcgc
     4501 gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt
     4561 tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac
     4621 gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg
     4681 attctgccgc tggatgggcg gaagaaactg gtcaatgccg atattgaaaa ggttaaacag
     4741 gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag
     4801 caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc
     4861 ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta
     4921 tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagttt
     4981 gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc
     5041 cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc
     5101 catccgattt cggttcagtt catcatgaag tcgaactagc ggtgttgatt ggcgcgacgc
     5161 tgcgtcaggc tacggaagag catgtccgca aagccattgc cggttacggc gtggcgctcg
     5221 atctgacgtt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg
     5281 ctaaagcgtt tgataactct tgtccgcttt ccgggtttat tcccgcggcg gaattcaccg
     5341 gcgatccgca aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta
     5401 cgactgcgga catgatccat aaaatcgttc cgctgatcgc ttatatgagc aagtttttta
     5461 ccctcaaggc cggtgacgtt gtgctgacag gcacgcctga tggcgtcggc ccgttgcaaa
     5521 gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat
     5581 actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag
     5641 gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa
     5701 accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt
     5761 tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt
     5821 cgccagctca atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa
     5881 cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct accaatgacc
     5941 tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctgcttact
     6001 ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa
     6061 tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa
     6121 atttaataga tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag
     6181 ccctgttttt gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg
     6241 taactctcaa tctttaaaaa acgtctgtat ttttaaatgt gataaacttt ataccttgag
     6301 tacatggaga gaataattgc tgatcagact tcaggtacct caaagagtgt cttcttaccg
     6361 tgtcttttct gatactcatt acaggtgtta atcattttat tggccgcttc ttttagcaaa
     6421 gaaagcatta aatcatcata atcaacatag aatctggttg tttcagtttc cgtttttatc
     6481 tccccgatgg cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc
     6541 gcttgtttaa ccgtgttaga cagggtggta aagaaactct gcacagattt taacttgttc
     6601 ttcaattctg gaatcagttt cccttcaact acgcccgcag caatagaata ggaaatgatt
     6661 aaaccaaatg gaccggcgac gacaccggct gcggcaccgg catacgcttc cttcctgatt
     6721 ttatctacct gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta
     6781 tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat
     6841 ttttgcgctt cattcagctt cgtgatgccg tcatccagta ccttaatgag aatgtctttc
     6901 tgggcggatg ctttcttctc attatactca tcaaatagca aaatatacgc tgcgagcaat
     6961 tgcgtcgcaa caccacacca ttcatacact gtttgggttg cttcaaaata cttatcctgg
     7021 ctatccatta gtaaggtttt aatattgcca actaaaacgg aggctgcctg tgaatactcc
     7081 tgtttaaagc gacttaactc ttttatggtt tcatcaaagg tctgccaggg gatgacctga
     7141 tcgagatatt tattataaag atccaatgct ccatctgcgg tttcgattgc gtttttaact
     7201 acctctaccg tttta
//
LOCUS       DAZFKY010000209         7211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFKY010000209 DAZFKY010000000
VERSION     DAZFKY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..510
                     /locus_tag="ACHKCL_004144"
     CDS             85..510
                     /locus_tag="ACHKCL_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5765000.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHKCL_004145"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHKCL_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(810..992)
                     /locus_tag="ACHKCL_004146"
     CDS             complement(810..992)
                     /locus_tag="ACHKCL_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765001.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            1321..2193
                     /locus_tag="ACHKCL_004147"
     CDS             1321..2193
                     /locus_tag="ACHKCL_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5765002.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2565..5414
                     /gene="cah"
                     /locus_tag="ACHKCL_004148"
     CDS             2565..5414
                     /gene="cah"
                     /locus_tag="ACHKCL_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO5765003.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            5525..>7211
                     /locus_tag="ACHKCL_004149"
     CDS             5525..>7211
                     /locus_tag="ACHKCL_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO5765004.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1845 a         1802 c         1974 g         1590 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatactatt ttgatgaaaa ttattatatg tttcctcaga ttttaatcag
      721 gcgcccagat taaaccaagt attgagcgcc tgattatgtt tattcattaa tatctgtcat
      781 tgattgtctt attatcagct atccccgtat taatcagagt tttcatttat ttttaatgca
      841 taatcagatg ccgcctcatc attatcctca gtattatcat agccgaaaac ttctcccgga
      901 cacgggcgtt cacgataata gtcatcgtca tagttttcat cgtcagataa tgatatgtcg
      961 tattctgaat atgattcacc acaattatac atgaaatcat ctcctttggg gggggcaata
     1021 attagtgagt ttattcgaaa aaatgatgag cgccagacca atccacccaa cttttgatta
     1081 taatcatgaa gatacttgtc tcagtacaaa ttgaatcaaa ctcaatgcag catgattcac
     1141 ctttatattc tctcagggaa accaaatacc actactcaac tattaagaac aaaattcggg
     1201 atgtatgatt atcagaagat acctgcatcc taaataattt cagacagata ttatcgttgt
     1261 gaatcccccg ccacctttcc ggtgcgcggg gttttgtctt ttttcaccgg gaatacatgt
     1321 atgaatccgt ctgatgccat tgaggcaatt gaaaaaccgc tctcctccct gccttactcg
     1381 ctttcccgtc acatcctgga acatctgcgc aaactcacca gtcacgaacc cgtgattggc
     1441 attatgggta aaagcggggc cggtaaatcc tcactctgta atgcactgtt tcagggggag
     1501 gtcaccccgg tcagtgatgt tcacgccggc acccgggaag tgcggcgctt ccgtctgagt
     1561 ggccatggtc acagcatggt tatcactgac ctgcccgggg tgggcgagag ccgggacagg
     1621 gatgcagagt atgaagccct gtaccgtgac attctgcctg aactggactt ggtactgtgg
     1681 ctgattaaag ccgatgaccg tgccctgtct gtggatgagt atttctggcg acacatactg
     1741 caccggggac atcagcaggt gctgtttgtg gtgacgcagg ccgacaaaac agagccctgc
     1801 catgaatggg atatggcagg tattcagcct tctcccgcac aggaacagaa cattcgcgaa
     1861 aaaacagatg cggtattccg gctgttccgg cccgtacatc cggttgttgc cgtatcggcc
     1921 cgcaccggct gggaactgga tacgctggtc agtgcgctca tgacagcgct tcccgaccat
     1981 gccgccagtc ccctgatgac ccgcctgcag gacgagctgt gcacggaatc tgtctggggg
     2041 caggcccgtg aacagtttac cggtgcggtg gaccggatat ttgacacggt ggagagcgtc
     2101 tgtgttgcct ctgttgcacg cacagttctg cgtgccgtcc gtgacacggt ggtctctgtt
     2161 gcccgcgcgg tatggaactg gattttcttc tgaacagtct gccaggggaa atgtctgatt
     2221 acattgcgtt aaccaactga aaacatgata caaacacctc atgcccctcc tgtttctctt
     2281 gcgtgactgc tctactgtta atagaataaa acgatcgata aaacaggtat cgatcgttta
     2341 tatcgatcgt ttatagtggt taatggcctg cgaaaaggca gctgaatctc ttcatcatgc
     2401 agaacggaat tgcacacaac agactgatac ttctctgtct gatgaagggg gagagcgttt
     2461 acgcccggga cgtatcgctg tgcccgataa ctctgttttg tacctgccgg tatccacttt
     2521 tgtgggtacc ggctttttta ttcaccctct gtaaggaaaa gctgatgaaa cgacatctga
     2581 acaccagcta caggctggta tggaatcaca ttacgggcac cctggtggtg gcctccgaac
     2641 tggcccgctc acggggaaaa cgcgccggtg tggcggttgc gctgtctctt gctgctgtca
     2701 catcagtccc ggcactggct gctgacaagg ttgtacaggc gggagaaacc gtgaacgatg
     2761 gaacactgac aaatcatgac aaccagattg tcttcggtac ggccaacgga atgaccatca
     2821 gtaccgggct ggaactgggg ccggacagtg aagaaaacac cggtgggcaa tggatacaga
     2881 atggcgggat agccggaaac accactgtca ccacaaatgg tcgtcaggtc gtgctggagg
     2941 ggggaacagc cagtgatacg gttattcgtg acggcggggg acagagcctg aacggactgg
     3001 cggtgaacac cacactgaat aacagaggcg agcagtgggt gcatgagggc ggggttgcca
     3061 ccggtacaat tatcaaccgc gacggttacc agagcgttaa aagtggcggg ctggcaacag
     3121 gaaccatcat caacaccggc gcagaaggcg gccctgattc tgacaactcg tatacgggtc
     3181 agaaggtcca gggaacagca gaatccacca ccatcaacaa aaatggacgg cagattatct
     3241 tattttccgg gctagcccgt gacactctca tttacgcagg tggtgaccag tcggtacacg
     3301 gaagggccct gaataccaca ctgaatggcg gttaccaata tgtgcacagg gacggacttg
     3361 cgctgaacac ggtaattaac gaggggggct ggcaggttgt taaggcaggt ggcgctgccg
     3421 gtaacaccac cataaatcag aacggtgaac tgagggtaca tgccggcggg gaagccactg
     3481 cagtcaccca gaacacgggc ggtgcactgg ttaccagtac tgctgcaact gtcatcggca
     3541 caaaccgtct ggggaatttc acggtggaaa acggtaaggc tgacggtgtt gttctggaat
     3601 ccggcggtcg tctggatgta ctggagagcc attcagcaca gaatacccta gtggatgacg
     3661 gcggtaccct ggcagtgtct gccggcggta aggcgacaag tgtcaccata acatccggtg
     3721 gtgccctgat tgcagacagt ggtgccactg ttgaggggac caatgccagc ggtaagttca
     3781 gtattgatgg cacatccggt caggccagcg gcctgctgct ggaaaatggc ggcagcttta
     3841 cggttaatgc cgggggacag gctggcaaca ccactgtcgg acatcgtgga acactgacgc
     3901 tggctgccgg gggaagtctg agtggcagaa cacagctcag taaaggcgcc agtatggtac
     3961 tgaatggtga tgtggtcagt accggcgata ttgttaacgc aggggagatt cgctttgata
     4021 atcagacgac accgaatgcc gcgctgagcc gtgctgttgc aaaaagtaac tccccggtaa
     4081 cgttccataa actgaccacc acgaacctca ccggccaggg cggcaccatc aatatgcgtg
     4141 ttcgccttga tggcagcaat gcctctgacc agctggtgat taatggtggt caggcaaccg
     4201 gcaaaacctg gcttgcgttt acaaatgtcg gaaacagcaa cctcggggtg gcaaccaccg
     4261 gacagggtat ccgggttgtg gatgcacaga atggcgccac cacagaagaa ggtgcgtttg
     4321 ccctgagtcg cccgcttcag gccggcgcct ttaactacac cctgaaccgt gacagcgatg
     4381 aagactggta cctgcgcagt gaaaatgctt atcgtgctga agtccccctg tatacatcca
     4441 tgttgacaca ggcaatggac tatgaccgga ttctggcagg ctcccgcagc catcagaccg
     4501 gtgtaaacgg tgaaaataac agcgtccgtc tcagcattca gggcggtcat ctcggtcacg
     4561 ataacaacgg cggtattgcc cgtggagcca cgccggaaag cagcggcagc tatggcttcg
     4621 tccgtctgga gggtgacctg ctcagaacag aggttgccgg tatgtctctg acgacagggg
     4681 tgtatggtgc tgcaggccat tcttccgttg atgttaagga tgatgacggt tcccgcgccg
     4741 gcacggtccg ggatgatgcc ggcagtctgg gcggatacct gaatctggta cacacatcct
     4801 ccggcctgtg ggctgacatt gtggcccagg gaacccgtca cagcatgaaa gcgtcatcgg
     4861 acaataacga cttccgcgcc cggggctggg gctggctggg ctcactggaa accggtctgc
     4921 ccttcagtat cactgacaat ctgatgctgg agccacaact gcagtacacc tggcagggac
     4981 tctccctgga tgacggccag gataacgccg gttatgtgaa gttcgggcat ggcagtgcac
     5041 aacatgtgcg tgccggtttc cgtctgggca gccacaacga tatgaccttt ggtgaaggca
     5101 cctcatcccg tgacaccctg cgcgacagtg caaaacacag tgtgagtgaa ctgccggtga
     5161 actggtgggt acagccttct gttatccgca ccttcagctc ccggggtgac atgagcatgg
     5221 ggacagccgc agccggcagt aacatgacgt tctcaccgtc ccggaatggc acgtcactgg
     5281 acctgcaggc cggactggaa gcccgtatcc gggaaaatat caccctgggc gttcaggccg
     5341 gttatgccca cagcgtcagc ggcagcagcg ctgaaggcta taacggtcag gctacgctga
     5401 atatgacttt ctgattatac ggtatcccgg aaatacgatt tcaggaaaat tcatgcagta
     5461 atgggaaagg acgctcattg ctgttcaccg cagttctgaa ttattgaatt tataaaggtg
     5521 aatgatgcac gaaaagaaca tcgccctgct ttgtgatgaa gccgaccggc ttttgcaact
     5581 gaatatcaat ctgctccggc aaatggttga tgagccggat gtgttatctg acagtaagaa
     5641 cgaaaacggg ctgctttttg ataaacggaa agcgctgaaa agaattgagg agctggaggg
     5701 cgaacaaatc aaaaccgccc gcagggagat ggtgctggct gtggtcggca cgatgaaggc
     5761 gggcaaatca accaccatca acgccattgt ggggcaggaa attctgccca accgtaaccg
     5821 ccccatgacc tcggtaccga cattgatccg ccacgttccc ggaaaaacgg aaccggtcct
     5881 ccatctggaa catattcagc ccgtccgcaa tttattaatc acactgcagg aaaaactcgc
     5941 caccccggca gggcagcaag tcgcacagtc cctgcagcaa accggggata cccgcgaact
     6001 actggatatt ctggcggatg atgtctggct taaaaatgaa taccacgggg aagacgaaat
     6061 ctttaccgga ctggcatcgt taaatgacct ggttcgtctt gctgcggcaa tggggactga
     6121 atttcctttt gacgaatacg cagaagtgca gaaactaccg gtgatcgacg tggcgttcag
     6181 ccacctggtg ggaatggatg catgtcaggg aacactcaca ctgctggata cccccggccc
     6241 taatgaagcc ggacaaccac agatggaaat gatgatgcgg gatcaactac agaaagcctc
     6301 tgcggtgctg gcggtgatgg attacaccca gatgaactcg aaagccgatg aggaggttcg
     6361 taaagagctt aatgccattg ctgacgtctc agccggtcgt ctgtttgtgc tggtcaataa
     6421 attcgatgaa aaagatcgca atggtgatgg tgcggatgcc gtacgccaga aggtgccagc
     6481 aatgctgaac agcgatgtgc tgcccgcctc ccgcgtttat cccggctcat cacgtcaggc
     6541 gtacctggct aaccgtgcgc ttcatgagtt acgaaaaaac ggtgcgcttc cggttgatga
     6601 agcctgggtc gatgatttta tcagggaagc tttcggacct atggtggaag aggacgactg
     6661 gaaagacagc actaaggtaa ataaaaaggc agaaaaactc tggaatatat ctctgatcga
     6721 tcaactgata acggaagtca tacagagctc ccattccaga gcggcggcac tggcggttga
     6781 ctctgccgcc gcaaaactga tgcagaatgc agaaaatgtc agcgaatatc tgtcattacg
     6841 tcatcagggg ctgcagcaaa gcattcagtc actacaggcg catattacca gcctgctggc
     6901 ggatatccag gagattgagg aatgtcagaa tcaggtgacc ggtgatgtca gaatggccat
     6961 ggaggacatc aataccaaaa ccggggaatt actgacgaag gtctgtgcct cactggagga
     7021 ggagctgaac gactatttca gaagcggtaa acgcaaagaa cagcaaatgc tggaggaaga
     7081 aaattcagcg cagccccggg agagaaacgc attcgctttc tttcacgata ttttcggaac
     7141 cggaaatcag catgacagga tgcgggactt tgatccggac agccccgaaa taaaattcag
     7201 tgatcgcagg g
//
LOCUS       DAZFKY010000210         7203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFKY010000210 DAZFKY010000000
VERSION     DAZFKY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(89..373)
                     /gene="ppnP"
                     /locus_tag="ACHKCL_004150"
     CDS             complement(89..373)
                     /gene="ppnP"
                     /locus_tag="ACHKCL_004150"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5765005.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(445..1122)
                     /locus_tag="ACHKCL_004151"
     CDS             complement(445..1122)
                     /locus_tag="ACHKCL_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5765006.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1380..1571)
                     /gene="yaiA"
                     /locus_tag="ACHKCL_004152"
     CDS             complement(1380..1571)
                     /gene="yaiA"
                     /locus_tag="ACHKCL_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5765007.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1621..2145)
                     /gene="aroL"
                     /locus_tag="ACHKCL_004153"
     CDS             complement(1621..2145)
                     /gene="aroL"
                     /locus_tag="ACHKCL_004153"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5765008.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2328..2786)
                     /locus_tag="ACHKCL_004154"
     CDS             complement(2328..2786)
                     /locus_tag="ACHKCL_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5765009.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2906..3715
                     /gene="proC"
                     /locus_tag="ACHKCL_004155"
     CDS             2906..3715
                     /gene="proC"
                     /locus_tag="ACHKCL_004155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5765010.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3732..4847)
                     /gene="adrA"
                     /locus_tag="ACHKCL_004156"
                     /gene_synonym="dgcC"
     CDS             complement(3732..4847)
                     /gene="adrA"
                     /locus_tag="ACHKCL_004156"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5765011.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4949..5269)
                     /gene="psiF"
                     /locus_tag="ACHKCL_004157"
     CDS             complement(4949..5269)
                     /gene="psiF"
                     /locus_tag="ACHKCL_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5765012.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5388..6803)
                     /gene="phoA"
                     /locus_tag="ACHKCL_004158"
     CDS             complement(5388..6803)
                     /gene="phoA"
                     /locus_tag="ACHKCL_004158"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5765013.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6904..7164)
                     /gene="iraP"
                     /locus_tag="ACHKCL_004159"
     CDS             complement(6904..7164)
                     /gene="iraP"
                     /locus_tag="ACHKCL_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5765014.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1744 a         1901 c         1729 g         1829 t
ORIGIN      
        1 aaaaaatcgc agcaaaggaa atattattat ggggttatga gaggacattt cagaaaaatg
       61 ccctcccgtt caagcggaag gcgaggaatt acagatagcg gcacagataa gaggtgggtt
      121 cggcaacttg cagatgaaac tcactgtgac cgggaacatt aaacaccgaa ccggcttcat
      181 acacttgcca gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca
      241 tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc aaccataaca cccacgctgg
      301 cgcgaccagt gctgctgctg gaaaagccga tcgatttcac tttgccggaa aagtactcat
      361 tactttgaag cataaactgg ccctgtctgg tagttgaata tctacttcac tataggggcc
      421 agatgcgctg gctgtcacgc aaaattacac cagtaattcc gcagccagcc gtgcaatcaa
      481 tacgttagag agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgctg
      541 gttaaatccc aaacaatcca gcatgatgac atctgcgcct ttcgccagta attctttccc
      601 ggcatcaatg attttttgtt ctgaatcatg aatggggtta cccaatgaaa atacaggcgg
      661 tttctgcaaa atttgccatt tttgcgcctg aacggtcagc aactcctcaa ccggtacgat
      721 aacccccacc tgatgatctt caacaataga ggaaaccagc ggtggcaata tcctcgacgg
      781 ctcaagaaag atcgtattac gcgcagtcat actactaatg tttgccgtac tcattaatat
      841 aatgacgtcg tagccctgat tatcgagcac ttcaaccaca ccttgcaggt cacgctctac
      901 tttgcgacgc gaaacatggg ccagatggtt gtcatttaat aatgtgagaa ttgtgtcttc
      961 gcctgcttct ggcgcgtact ccgccatcac ttcttcgcgg cttaacttcc ccagcaggct
     1021 atgatgggaa atattatctt cgtcaatgta ttctgtcagg agcggcaaaa cttcctgcat
     1081 aggtacaatg ccgatggtta gaatcgccaa cgacgcactc attgttcttc caccttactt
     1141 ttatttcgtc gttaattacc gggcaagtgt taagcaccac tgctgacact tacttgtgct
     1201 acaaatgaca aaaagcgtag cagcagacgt gcacggcata atgtaaagat tccaaatgat
     1261 tccagtaatg gattgttatt gtttaatatt ctaattattt gaaaaacatg aattatgaaa
     1321 aatgtgacgc agatcccttg tagtggcact cagaatccct tccgagcagt ctgatgcggt
     1381 cactctttgt cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agcggtcgga
     1441 tgttcactga tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc
     1501 gtctgtcctg gctttccttt ttcaattgtc actatatatg cttcacgagg ataaggcggt
     1561 ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctcgaaaa
     1621 tcaacaattg atcgtctgcg ccagggcgct gcgaatttca gaaatcacct ggctgggttc
     1681 gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag
     1741 cacttcctga acttcttcgc tcagcggttt tcccgttaag gttggccgta aatcttcttc
     1801 cggtgcagct tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat
     1861 cccgttattt tgcatgaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc
     1921 gataacggtg gatggcgcag ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc
     1981 cgcccactct tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca
     2041 ctgatcggta tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt
     2101 tttaccacag ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca
     2161 ataggtcgca atgcggcgaa agccggtgtc atgagaatag cgatcatacc atcaaactag
     2221 tacaatttcg attgtaaaga aaaaattcca cttaaagtga aaatctcaat acatccctta
     2281 ctataccaat aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta
     2341 cgttgcactt ccagccacca cttctccagc tccgcggcaa aggcctggcg gtcacgttgt
     2401 gaaaggctat ctggtccgcc ggtctggatc ccactggcac gtaaggtatc cataaaatcg
     2461 cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac gttcaccgcg cggattaagc
     2521 gccgcagcgc ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa
     2581 tctcccgctt cacactgccg gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg
     2641 cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc
     2701 agcggcatct gcatacgttc cgccgcgcga tacaaaatct ctttaattac attgggacac
     2761 gcgtcggcat ccacccaaat tgtcataaag tcatcctttg ttgggtaatc ctctattgtg
     2821 tcgcgctttt gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa
     2881 tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa
     2941 tatgggaaaa gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat
     3001 ctgggtatat accccctccc cggataaagt cgccgccctg catgacaagt tcggcatcaa
     3061 cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac atcatttttg ccgccgttaa
     3121 acctggcatc atgattaaag tgcttagcga aatcacctcc agcctcaata aagactctct
     3181 ggtcgtttct attgctgcag gtgtcacgct cgaccagctt gcccgcgcgc tgggccatga
     3241 ccggaaaatt atccgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc
     3301 cgtaacgcca aacgcgctgg taaccccaga agataccgct gatgtgctga atattttccg
     3361 ctgctttggc gaagcggaag taattgctga gccgatgatc cacccggtgg tcggtgtgag
     3421 cggttcttcg ccagcctacg tctttatgtt tatcgaagcg atggccgacg ccgccgtgct
     3481 gggcgggatg ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc
     3541 aaaaatggtg ctggaaacgg gagaacatcc gggggcactg aaagatatgg tctgctcacc
     3601 gggaggcacc accattgaag cggtacgcgt actggaagag aaaggcttcc gtgccgcagt
     3661 gatcgaagcg atgacgaagt gtatggaaaa atcagaaaaa ctcagcaaat cctgatgact
     3721 ttcgccggac gtcaggccgc cacttcggtg cggttacgtc cggctttctt tgctttgtag
     3781 agcgccaaat ctgccgattt caaccactcg cgatagtgac tcatttgtgg gttcagcggc
     3841 gcaaccccca cactaatccg taaatttacc tgtggcgtat tcggcaaacg taatgtattt
     3901 agcccttcat gcacccgtaa catggcggta atggcgctct cagctggcgt accggacatg
     3961 attaccgcaa actcatcgcc gccaaaccga ccaatcacat cgctaccgcg cagggtaatt
     4021 tgtaactgtc gggtaagcgc cacaatcgct tcatcgccca catcatggcc ccaggtatcg
     4081 ttgatgctct tgaaatggtc gatatcgata atcagtaacg ttgcatcgcg attatgccgc
     4141 cgacagttat caaattcatt gcgtaacata gtttcccaat gacgtcggtt atacacgccc
     4201 gtcatgccgt cgcgggtact catgacctgc aacctgcgtt tatgttccgc cagtttggtt
     4261 gccgtctggt agctgaccca gccaaacagc agaggataaa tgacaataat gggaagggag
     4321 agccaccatt ccagcggcgc actattgaac gacacggtaa tgcccgtcag ctcgagggtg
     4381 acaaggcagg aaaccaccat caacaccaga cccgcgacaa acagacgggg gccgcctgcc
     4441 cccatcaaat tcagacacat aatcatcaac atcgcggtgg aaggcagcac gtttacgccc
     4501 attacgccta cccacattcc cgctaatact gcatcggttt ttaagttgta aatttcccgg
     4561 ctaagcggat cgacggccct gctcgctatc tgccaggcta aatgcggcca gacgaacgcc
     4621 cagccgacca acaccagcca ccaccagccc ggcggcgggt gtgaaaccag cgttgaagca
     4681 atcggtaaga acatgccagc caggccaacc gcacggggta gtctgacgcg acgggcgaag
     4741 cgcagcccgg accgctgatg ttcgttttga ggagttaaag gaggttcctc cccgtgcgcc
     4801 gccgcttttt tgaaaaagtt ttcatcattc attatttttg ggaacattca gaaacaattt
     4861 tcccaaatta tagagacggg gctaaaggca gaagtatgaa atttcgggtg agccatttta
     4921 gtagctcacc cgttagcact atcaggtatc aggcggcttt cttgaggcag gcgctcataa
     4981 acttattacg atcatcacct ttcagtgatt gttgcgttgc ttgattatta cattcgcgca
     5041 tcttttgctg ctgtggcgtc aaactttttt caccaggcgc agacttgctg ttcttcaggc
     5101 aatcactcat gtaggtctta cgagcatccc ctttcaacgc ctgtgccgtc gcctgctgat
     5161 tacaggaggt catacgctgt tgttgtgggg ttaaggttct ctcggcagcg ccgacggtgg
     5221 ttaaaaaaac cagaccgaaa agcaaggtaa ccagtaatgt aattttcata gcaccatccc
     5281 tcttcatgtt ttaaccatga gcgtatgcgc ccgtgatctg acattaagtc tggttgctaa
     5341 cagcaaaaaa accacccggc agcgaaaatt cactgccggg agcggtttta tttcagcccc
     5401 agagcggctt tcatggtgta gaagagatcg gtctggtcgg tcagtccaac gacattggcg
     5461 gcatgcgggc catacgccgc aatacgcaac tgactgccgg tatgttcttg tgaatcctct
     5521 tcggagttcc cgtaactcat caccatcact gcgccatctt tggtatttag cgcctgagtg
     5581 aggcccggag ctttggtatc cggcgcaaca atctggctgg cgtgggcgtg atcagcggtg
     5641 actatgacca gcgtgttacc atcctttttg gcgaattcca gcgcccgttg tacggcttca
     5701 tcgagatcga ccgtctcgcc aatttgccca caaggattcg cagcatgatc ctgtttatcg
     5761 attgacgcac cttcaacttg caggaaaaag cctttctcat ttttactcaa caattcaatg
     5821 gctttgtcgg tcatctgtgc cagggtcggt acactgtcat tacgttgcgg attaggcgta
     5881 caggtgactg cgggcttatc gatattaccg tggtacgttg ctttcggtcc tagccagcgc
     5941 actggcatat tgccgtcagc aaacaggcca agcaggggtt tttgctgatt cgcttccgtc
     6001 accgaattca gtgaggcagc atcgctcacc aactgataac cacgcgcctg tgcctgttca
     6061 cgcagcgttt ttccctgcca ttcaccagcg gttgccgttt cagcaaaggt ttttgcgccg
     6121 ccgccaagcg taacgtcggc acgagcgtta agcagctgtt cggtaatcga tccttttccg
     6181 cctttttcca gagcgttacc cggacatttt tcactggtcg cgctcggacc gtagcatttg
     6241 cgcgaggtca catgcgccac cagcgcggcg ggcgtggcat cctgcaactc tgcggtagaa
     6301 acgttaccgg tcgccagacc tgcggctttt gccatttcca gaatcgttgg gtgatctttt
     6361 tcgtgaatat cgacgcccag cgcgccgtta taggttttga caccggttga ccaggcggtt
     6421 gctgatgcag ccgagtcggt gacgtagtcc ggttttccgg tttttttatt cagcgcatag
     6481 tgagtgtatt gcccggtaag cggtaaggca tcgatacctt taaaaaagcc gcccgcacct
     6541 tcggcataat tacgtgcggc agtaatttcc gaatccccca tcccatcgcc aatcagcaaa
     6601 ataatatttt ttgcaggttt atcgctaaga gaatcacgca gagcggcggt ctgatcagcc
     6661 gttaagcggc gagcaccgcc gggtgcagta atatcgccct gagcagcccg gttttccaga
     6721 acaggcattt ctggtgtccg ggcttttgtc acaggggtaa acagtaacgg taagagtgcc
     6781 agtgcaatag tgctttgttt cactttattt tctccatgta caaatacatt aaaaaataaa
     6841 aacaaagcga ctataagtct cggccgtgac aactttatga cagctgttga aaagattaac
     6901 tttttactga cgaggatgct tcaataactt ctttacgtaa tcgcgcagca gctccgtatc
     6961 gtcgtcagga atgctggcat cgggctttac ctcgtacagc gccccctcta cctgatcaat
     7021 caaccgctgt tggtcatttt gcgccatatt gcgaagcatt gcagtgacga taatctccaa
     7081 agcttctacc tgcgcacaca gttctttcga ctcttcttct ttttgggcaa gcttaaataa
     7141 caactcagca atgagatttt tcatgtctgt atttccttat ccaaagtatg gagaagttaa
     7201 cat
//
LOCUS       DAZFKY010000211         7187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFKY010000211 DAZFKY010000000
VERSION     DAZFKY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(583..1389)
                     /locus_tag="ACHKCL_004160"
     CDS             complement(583..1389)
                     /locus_tag="ACHKCL_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5765015.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1390..1695)
                     /gene="ccdB"
                     /locus_tag="ACHKCL_004161"
     CDS             complement(1390..1695)
                     /gene="ccdB"
                     /locus_tag="ACHKCL_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO5765016.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1697..1915)
                     /gene="ccdA"
                     /locus_tag="ACHKCL_004162"
     CDS             complement(1697..1915)
                     /gene="ccdA"
                     /locus_tag="ACHKCL_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO5765017.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2458..3057
                     /locus_tag="ACHKCL_004163"
     CDS             2458..3057
                     /locus_tag="ACHKCL_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5765018.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3054..3674
                     /locus_tag="ACHKCL_004164"
     CDS             3054..3674
                     /locus_tag="ACHKCL_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765019.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3671..3871
                     /locus_tag="ACHKCL_004165"
     CDS             3671..3871
                     /locus_tag="ACHKCL_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765020.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4279..5256
                     /locus_tag="ACHKCL_004166"
     CDS             4279..5256
                     /locus_tag="ACHKCL_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5765021.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5541..6281)
                     /locus_tag="ACHKCL_004167"
     CDS             complement(5541..6281)
                     /locus_tag="ACHKCL_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5765022.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6402..6632)
                     /locus_tag="ACHKCL_004168"
     CDS             complement(6402..6632)
                     /locus_tag="ACHKCL_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO5765023.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
     gene            complement(6801..7085)
                     /locus_tag="ACHKCL_004169"
     CDS             complement(6801..7085)
                     /locus_tag="ACHKCL_004169"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5765024.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGATLQMMKSKHLK"
     gene            complement(7115..>7187)
                     /locus_tag="ACHKCL_004170"
     CDS             complement(7115..>7187)
                     /locus_tag="ACHKCL_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5765025.1"
                     /translation="QEHLLLQLCMRVTKKLTDILMPP"
BASE COUNT         1812 a         1856 c         1800 g         1719 t
ORIGIN      
        1 acccggggcg gttcacagca tggataaagg cctacaaggc gctctaaaaa agaagatcta
       61 aaaactataa aaaaataatt ataaaaatat ccccgtggat aagtggataa ccccaaggga
      121 agttttttca ggcatcgtgt gtaagcagaa tatataagtg ctgttccctg gtgcttcctc
      181 gctcactcga gggcttcgcc ctgtcgctcg actgcggcga gcactactgg ctgtaaaagg
      241 acagaccaaa catcatggtt ctgtgttcat taggttgttc tgtccattgc tgacataatc
      301 cgctccactt caacgtaaca ccgcacgaag atttctattg ttcctgaagg catattcaaa
      361 tcgttttcgt taccgcttgc aggcatcatg acagaacact acttcctata aacgctacac
      421 aggctcctga gattaataat gcggatctct acgataatgg gagattttcc cgactgtctc
      481 gttcgcttct cagtggataa cagccagctt ctctgtttaa cagacaaaaa cagcatatcc
      541 actcagttcc acatttccat ataaaggcca aagcatttat tctcaggata attgtttcag
      601 catcgcaacc gcatcagact ccggcatcgc aaactgcacc cggtgccggg cagccacatc
      661 cagcgcaaaa accttcgtgt agacttcctt tgaactgatg gacttatgtc ccatcaggct
      721 ttgcagaact ttcagcggta taccagcata cagcatgtgc atcgcatagg aatggcggaa
      781 cgtatgtggt gtgaccggaa cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc
      841 ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt
      901 ccttcctgtg cgacggttac gccgctccat gggtattttc agtgttgcca ccatcgtctg
      961 cagctggctg acgtaccagg agtcagagag cggaaccagc cggtgagtct gctgaccggc
     1021 gggcattctc cccgccgtcc tggcagcttt ttcggtccgt tgtttcaggg tcgcaagctg
     1081 cacaaacgga tacggaggcg caagcgaaaa atccccccgc gtcagcgcca gtgcttcatt
     1141 aatgcgtgct ccggtgttcc acagtgtggc cagcagcatc ttgcggtgca gattcgggac
     1201 gtaatggagc agggcactca cttccggagc cagcagatat tttggcagtt catcatggac
     1261 catcgacatc tggcgaagtg ccagagctgc cggataatca atggcaacag gcagcgatgc
     1321 aggctgtccg gcagaataca ctgccggtac catgactgca gactggctgt gtataacgga
     1381 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
     1441 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
     1501 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
     1561 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
     1621 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
     1681 aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa
     1741 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
     1801 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatatg
     1861 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac
     1921 acctcttttt gacatacttc gggtatacat atcagtatat attcttatgc cgcaaaaatc
     1981 agcgcgcaaa tacgcatact attatctggc ttttagtaag ccttatgtat tttacctttc
     2041 gttatgttaa ccaataaaaa ttaaaatctg ccttataaaa acaaagcgta attaccgcat
     2101 tcccgtttcg tatggatccg actttatttg aataaacgac tttccggaag tgtaccacct
     2161 gagaaaatca tcatatcgct gttttccgcc cctgcgcggc gcatcacagt ccagagatac
     2221 aacacaacat atagtgccat gggcaactga atgaggcact gcatgctgtg ctgacgggat
     2281 gtaacgctgt gcggagggag aggaagatcc gtaaccacca gaaaccggat ctgaccagga
     2341 aaagagaaag agtgtatgta agttctgtac tgtatgtatg aattgtattg tatgtattgt
     2401 gtatttttca taccgggcat acgatggcgc cattccctga gaaaccggag ccagaccatg
     2461 acatccctct ccccggacac cgtccgccgc attgaagacg ccgccgccgc cctgatcgcc
     2521 gccggcaccc cgaacccgac caacgagcag gtccgtcagc acctcggcgg cggctccctg
     2581 tcccacatct ccccggtgat gcgcgcgttc cgcgcccgcc agcgcgagca ggccacgccc
     2641 ctgccgccgg agctggcgca gctgctgacc ggccagctgg ggctgctgtg gcaggcggcc
     2701 gtgaaacagg ctgaagccgg ggccctggcg gcgcgcgagc aggccgacga cgacattgcc
     2761 cgggccgaca aggagcggga tgaggcgctg gcgaacgtgg ccgcgctgga aagcgagctg
     2821 gcggtgctgc gcgaggtggt ggccgaacgt gaccgcctgc tgcaggaggt acgcgagcta
     2881 cgcgcggagg cgctgccgct gcgcgagcag gtggcccgcc tgaccgccac cggcgaacac
     2941 ctggcggcgc agctgcagga cacgaaggcg gagctgaagg aagcccggga ggacggccgc
     3001 cagcttcaga ccgaactgct ggctctggcc cgccaggacg ggaaggtgaa gaaatgagac
     3061 aggaaagagt attacgtata aaggcatttc gacgggcact ggagcttggt ggcggtgaac
     3121 gggctgacgg gtgtcgtcat tttccccgct ggaaaacgga actgaggaat ttcccgcaca
     3181 gcagctgcga tttagccagc aatactctgg cgtactacct caaagaacga gagagatgcc
     3241 atccgtgcat catttttatg gaagggaacg ctggctttca tgaagcggaa aactcaacag
     3301 ttcacgggca tgtcatcgta ttgctggacg gagagtacat cgatttgacg ctggatcaat
     3361 tcgacgaata tccggactac attccttacg aacctgtcga atctgacggg cagattggta
     3421 agctgttgcg taatatcatg aagcatgagg acccggtaaa aacccgccag attgatctcg
     3481 atggtggcga agagctgtat gcctggttac gcgataccgc cgacgaagtg ctggcagcag
     3541 atccggaatg gcaggcccgg gaacggtcga tagaagaagc aagggaagcc gctattacgg
     3601 tgttcccgtt tctgtctgac gtgcaaaaaa ctgagtgcga acagggcccg gacagtcagg
     3661 aggctgccag atgaagattt atcattacac tgacctgaac ggcctgaagg gcatcatcga
     3721 atcaggctca ctgtgggcga cgcacttttc ctttctcaat gacagtaatg agctgactca
     3781 cggcatgaac tgccttgaga atgccctgca gtacctgagg gacgacttta acccgaaaac
     3841 ccttaagttc atcgaacagg cacccttata agcggttgtg tatgctgttt ccctggaatg
     3901 atttatccac atatccggag gctcgatcca gtaaacagat ccatgaatga tcaacaaagg
     3961 atccattaaa gatccccata ccgctgcaaa ccttgtcact catgggccgg gaccacgatc
     4021 acataagcag tggcatgtta ctgataaact gtaacatgct aatgataagc tgtattcagt
     4081 aatccatata ctgaagtaag ttaatgacat aaactatggt cagtacgcca gactcagctg
     4141 ttaaatacag gctgcaggtt tttcttcagt cagttagcgg ggctctgaca cacgatttgc
     4201 tgtttattct tttactgtcc acaggcagga ggctttctgg aaaacgaaaa ttcagacatc
     4261 aaaaaactgt tcggcgaggt ggataagtcg tccggtgagc tggtgacact gacaccaaac
     4321 aataacaaca ccgtacaacc tgtggcgctg atgcgtctgg gcgtttttgt accgaccctt
     4381 aaatcactga agaacagtaa aaaaaataca ctgtcacgta ctgatgccac ggaagagctg
     4441 acacgtcttt ccctggcccg tgctgaggga ttcgataagg ttgagatcac cggcccccgc
     4501 ctggatatgg ataacgattt caagacctgg gtggggatca ttcattcctt tgcccgccat
     4561 aacgtaattg gtgacaaagt tgaactgcct tttgtcgagt ttgcaaaact gtgtggtata
     4621 ccttcaagcc agtcatcacg caggctgcgt gagcgcatca gcccttccct gaaacgcatt
     4681 gccggtaccg tgatctcctt ttcccgcacc gatgagaagc acacccggga atacatcacc
     4741 catctggtac agtcagccta ctacgatact gaacgggata ttgttcagtt acaggctgat
     4801 ccccgccttt ttgaactgta ccagtttgac agaaaggtcc ttctccagct taaggcgatt
     4861 aatgccctga agcgacggga gtccgcccag gcactctaca cctttataga gagcctgccc
     4921 cgggatccgg caccggtatc gctggcgcgg ctgcgtgcac gcctcaatct gaagtctcct
     4981 gtattttccc agaaccagac ggtcagacgg gcaatggagc agctgcgcga gattggatat
     5041 cttgattaca cggagatcca gcgggggcgg acaaaactct tctgcattca ctaccggcgt
     5101 ccccggttaa aagcaccgaa tgatgagagt aaggaaaatc cgttgccacc ttcacctgcg
     5161 gaaaaagtca gtccggagat ggcggagaag cttgccctgc ttgagaaact gggcatcacg
     5221 ctggatgacc tggaaaaact cttcaaatcc cgctgaacat aaactgtagt cagtgaagag
     5281 tgttccttta ctgactacag cttatattat caggtgcagt gagtggtctg ctcactgcag
     5341 tttatattca gtttcctgca gtgctgcctg tagctgagct gtcatctgcc ggtcccttac
     5401 gtgagtcacc ccgtaacctg atgctgaggc attgctccct tcataaaaca tgacttactc
     5461 actacagctt atatacatgc tccagcttat gttatgtctg ttctgctgac cacagcttat
     5521 ataaggaagc gcgtatgccg tcacctcagg ggaggcagtg tacgcaggat ctccgcagca
     5581 tcccgcccgt cacctgtgaa aggcactgcc agcgtggcag ccatatccag cgcaaacact
     5641 ctggtataaa cctccatcga acgtggatcc ctgtgacctg ccagggcctg gatgactttc
     5701 cggggctggc ggtgatagag catatgcatg atatagctgt gccggaaggt gtgtggtgtg
     5761 accggaatcg aaaagtgtac tccgtcagct tcggcccgtc tgacagcctg cttcagccag
     5821 ttgcgcatgg tttcgtcggt cacggcccat aatggttcac gacgacgggg ccgggtggtg
     5881 atcatccagc tttccatctg cctgacatag cttatatccg tcagcggaac caggcggact
     5941 tcatcttttg gcgggcgtcc gcgtcgcgca cgcacttttt cggacaggat ccgcacaaac
     6001 ggccttgctc catccaggtc aaatgattcc ggcgtcagca tccgggcttc gccaatacgc
     6061 attccggtat tccagagggt ggcgaacagc atatggtgac gctgatccgg catatagaaa
     6121 agaagggcac tcacttccgg ggccagcagg taggccggtg tggcccctgt ggaagtggcc
     6181 attcttctca gggagagcgc tgttgcaaaa tccacccccg gcgcaatggg taacaggaag
     6241 acgcgttctg gtgaattctg caggggaatg acattgttca tgagttattt ttcacgtagc
     6301 acacgcactg tttatggata caggtattct gacctgaacg cccggatcct tccacgacaa
     6361 taaatcgcag cgtgcggatc cttttgtttg cttatcacgc attattgcgg gtaaaaccaa
     6421 atacgtaaag gtacgttttg cagcagggat cccgtattaa gccagtcgtg gcgcgggctg
     6481 agggctggtt tgccgttttt gggagaattg gggtaatgcc aggacgctaa tgatctatca
     6541 atggatggat gtaagtgaca attgaaaact gaaaaatgcg ctatgtagat tctgacagat
     6601 ttatgcacct gaaatacgtt caaaatccac atagcgtaac tctgattttc cttaccagaa
     6661 aaaagagggg gaataaccag tatctatccc ccctacacaa ttgcagaata ataaattata
     6721 aaaataagaa ctcaggattt atatacatga ttacatgtaa tattactgag aaatattttg
     6781 agataaaacc tttttgctga ttattttaaa tgttttgatt tcatcatctg taaggttgct
     6841 ccatattttt ttaaatagcg tccatatatc cttgtggtcc gctgtaaata caaattaccg
     6901 tctgaagtca gataaatatt cttactacgt cgatcttcct tactttccat acgtttaata
     6961 aaaccaaatt tttccagaga atcacaagct ctggtgatat atgttctgga aacatctaat
     7021 ctttcactga gttcagtcga agataagcag aaattatcag tactggataa caaggctaga
     7081 agcataagcg ttgtatcgtt tataccatcc cccttcaggg aggcattaat atatcagtaa
     7141 gttttttggt aactcgcata caaagttgta aaagaagatg ttcctgt
//
LOCUS       DAZFKY010000212         7157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFKY010000212 DAZFKY010000000
VERSION     DAZFKY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..437
                     /locus_tag="ACHKCL_004171"
     CDS             <1..437
                     /locus_tag="ACHKCL_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765026.1"
                     /translation="VELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSE
                     NAYIRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEK
                     DAQAYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKRGE"
     gene            589..777
                     /locus_tag="ACHKCL_004172"
     CDS             589..777
                     /locus_tag="ACHKCL_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765027.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAEQNRWKNHLRTKGE"
     gene            779..994
                     /locus_tag="ACHKCL_004173"
     CDS             779..994
                     /locus_tag="ACHKCL_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765028.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKILRSNKGE"
     gene            996..1214
                     /locus_tag="ACHKCL_004174"
     CDS             996..1214
                     /locus_tag="ACHKCL_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5765029.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            1216..1503
                     /locus_tag="ACHKCL_004175"
     CDS             1216..1503
                     /locus_tag="ACHKCL_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765030.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            1507..1638
                     /locus_tag="ACHKCL_004176"
                     /pseudo
     CDS             1507..1638
                     /locus_tag="ACHKCL_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1651..2130
                     /locus_tag="ACHKCL_004177"
     CDS             1651..2130
                     /locus_tag="ACHKCL_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5765031.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVNRG"
     gene            2123..2407
                     /locus_tag="ACHKCL_004178"
     CDS             2123..2407
                     /locus_tag="ACHKCL_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5765032.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLFNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            2479..2778
                     /locus_tag="ACHKCL_004179"
     CDS             2479..2778
                     /locus_tag="ACHKCL_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765033.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAVRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            2864..3148
                     /locus_tag="ACHKCL_004180"
     CDS             2864..3148
                     /locus_tag="ACHKCL_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5765034.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            3201..4511
                     /locus_tag="ACHKCL_004181"
     CDS             3201..4511
                     /locus_tag="ACHKCL_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5765035.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYADRFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEIGSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSSGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            4508..5086
                     /gene="wrbA"
                     /locus_tag="ACHKCL_004182"
     CDS             4508..5086
                     /gene="wrbA"
                     /locus_tag="ACHKCL_004182"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5765036.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEK
                     AGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            5107..5334
                     /gene="yccJ"
                     /locus_tag="ACHKCL_004183"
     CDS             5107..5334
                     /gene="yccJ"
                     /locus_tag="ACHKCL_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5765037.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5372..6613)
                     /gene="agp"
                     /locus_tag="ACHKCL_004184"
     CDS             complement(5372..6613)
                     /gene="agp"
                     /locus_tag="ACHKCL_004184"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5765038.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(6904..>7157)
                     /locus_tag="ACHKCL_004185"
     CDS             complement(6904..>7157)
                     /locus_tag="ACHKCL_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765039.1"
                     /translation="EVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYL
                     INTGNHYDVYLPPELFAQAYELNNKERNAQIGFLTRYAI"
BASE COUNT         2047 a         1648 c         1761 g         1701 t
ORIGIN      
        1 gtgttgagct tgaagcagca gagaacaacc ttattgatag tgaatgccat gttgctgaac
       61 tggaagaagc tctacgcgat aagcaggcgt tacttgaagc ctcagaaaag cgcaacgcaa
      121 aattacaaag cgagaatgca tacatccgca accggtacaa agaactggac ctattaatcg
      181 ggaaaaacat tctggtcatg caggctgcca ttatcgaatg gcaggcaact ggcgacgcta
      241 agagcggact agcatggatt tataacacac tgtttggccc tggcgaatta ccggacgaat
      301 ctgagaaaga tgctcaggcc tactttaatc gcaaatatgc accgattgac gaaaagctta
      361 tggcgcttca caagtggttt tgggaacaaa gtgaagccga gcgcgccgct ggcattcgca
      421 tcaaaagagg tgagtaatgc gtgtggcatg tatcggcttg ttaccgtatc cgactcgttt
      481 ttgggcttct gcgctaattg caaagaacat catcccgaca ccaaagcgcc gccataccgg
      541 tattgcagcg gcacgacgag cagcaaagag atgcaggaga gcaaaacgat gaaaaaccgt
      601 aaagcaaaga ttctgttagt tcgtagaaac gctcctggcg tctggcagtg ggtgagactc
      661 agcaaccgac ggatggggtt gatgaaatat tacgggatga tggattgtgg tttttgcaaa
      721 aagcccagcg cggagcaaaa ccgctggaaa aaccacttgc gcactaaagg agagtgatat
      781 ggctattgct gcaagttaca ccatgcatct ctattgtgat tgcctccagt gtacagatgg
      841 caaatataag tcgccagact tcggtgagta tatcggtacg tcatgggctg gctgtgcaaa
      901 agaggcgcgc aaggatggct ggcgaataag caaagacaaa acgcgtgctt ttgcgccagg
      961 gcataaaatt ttgaggagca acaaaggaga gtgatgtgcc tacattattc agaaaagaat
     1021 atccgcgaaa aagtagagcg acagaatttt tgtttctcat tctgtttatc gtgttgatga
     1081 caccgatatc cccgctaatt tttgtctggg caatcgggaa aataattgag ctagttattg
     1141 agttgtataa cgacgtggta tgggcgtcgt tcaacacact gcacaataaa attaacccat
     1201 ataaggaaaa ctgaaatggc aactttgcag gaattaatcg acctgacgcc agaacaggaa
     1261 aaagcgtgga atcgtcttgt gaaggctgta aaggatttca gggcagccgg aggaaagttt
     1321 tatagtgtcc tagacacgct gagcgcatac aacggcgagc acgttgccag cattgataac
     1381 gataagggct accacactgc aagcgtctat atgcctagca ttgatgcgcc tgggttaacc
     1441 agttgggctg atgattggca cggcatcacg ctgaaagatg gcgttgaagt ggataaggac
     1501 taacacatga caacgttcac cgacaaagaa atgattaaag aaatcaaaga gcgaatcggc
     1561 agcctggacg tgcgagataa tattgagcgc cgtgcttatg aaattgcgtt gactgcattg
     1621 accactgaac cattcgccac tatcgacaca gtgggaattg agctcgttaa atatggttgt
     1681 aacacgttta tttgtcccga caattcgatg gagccgggaa atgtaccact atatatcggc
     1741 ctgccacgaa ttgatccagc aagccagact gccaagctgt cattccagga atggttgtca
     1801 gaacaaaaag aaaaaataga cgttgattgc ggatgtgtaa gcatcgaaac gcttacgcac
     1861 tggatgaaat ccgcttatga ggctggtaac tctccggtaa ctccggatag ttggataagc
     1921 tgtagtgatc gaatgcctga aaagggccag aacgtgctta tttcggtgaa tttcgatagc
     1981 tctctggttg aaccgctaat atgctccgca cgctataccg gaagcacctt tcggcgcgga
     2041 gatgcaacga ttaagccggg taatggtatt gagcaagcaa ctcactggat gccgctacca
     2101 gaaccgccgc aggaggttaa ccgtggctaa cctgcaactt gccgttaaag gtgaatactt
     2161 cgatgccatg attcgcggag agaaaacgga agagtatcgc ctgtttaatg actactggaa
     2221 taagcgaatt atgttccgcg agtatgaccg cctgattatc acaaagggat atccgaagcg
     2281 cgacgattcc agccgaagaa ttgatgttcc gtatgacgga tatgaaatca agacaatcac
     2341 acatccgcac ttcggtgata aaccggtaaa ggtgttcgcg ataaaggtga atatcggcaa
     2401 tgaataacaa tcctcgcact cgcggggatt tcttttatct gaactcgcta cggcgagttt
     2461 tgttttatgg agatgattat ggcctgttca acattcaacc ctctaacgtt acagaaatac
     2521 cagccagacc ctgaagattt atgctcactg tgtggcggaa atcatggcaa agccgccatg
     2581 atcgaatgta aagacaaaat ccacatttgc cttaattgcg ttgatgtcct cgttgatatc
     2641 aaaaatgaga gagaagataa aaagcgtagc gaggctgttc gcgccttaga ttcatggatg
     2701 cgagatgggt atagtgccgc gcaaatttat gacttagcaa tatcaaaagg cgaaatacca
     2761 ggagtgcgca tcgaataaga cgtaaccaat attcgaattg aagaactgaa agaacaccaa
     2821 gccgcctgat ggcggttttt tcttacacat gcagaggcaa cttatgcgcg agttagtaaa
     2881 ccaacataac catggcattc agccagtcat cacacctgtt gtacagataa atgcgaatga
     2941 atgggtaaca ctggagcttt taatggctgt aacaggcctg agaaaaggaa caatattacg
     3001 cgccagggac agtgcgtgga tgaacggcag agaatataaa caaatcgccc ccgacggaac
     3061 gccaaagaaa aacagcgaat gtctctatca tcttcctacc atcaacactt ggatcaaaaa
     3121 ccaaccctta ccatctcagg atgtttaatt cttgtccata agagtataac ctgagcgtgc
     3181 tcttggacgc aggaggaaca atggcgaatt cagcctatcc agccggcgtt gaaaatcacg
     3241 gaggaaaact ccgaataacg tttaagtaca ggggtaaacg agtgcgcgaa aatcttcgcg
     3301 tgcccgatac accgaaaaac agaaagatcg ctggtgagtt aagggcttcg gtctgctttg
     3361 caatcagaac aggaacgttt gattatgccg atcgattccc tgactcacct aacctgaagc
     3421 tatttggcct ggtaaaaaaa gatatcaccg tcggtgaact ggcacagaaa tggcttactc
     3481 tgaaagcaat ggaaatcggt agtaacgcct taaatcgtta tcaatcagtg atgaaaaata
     3541 tgctaccgag gcttggtcct ggcaggctgg cgtcatcgat tacaaaagaa gatctgctgt
     3601 ttatcaggaa agatttactg accggggaaa agggaagcag gaaaaccagc acgtcccgaa
     3661 aaggaagaac cgtacccaca gtgaactatt acatgacaac aacagccgga atgttcagct
     3721 ttgccgccga aaacgggtat ctggagaaaa acccgtttaa ttcaataaca ccgctgagga
     3781 aatcaaaacc agtgccggat ccactgacca gagatgagtt tagccgtctc attgatgcct
     3841 gccatcatca acagaccaaa aacctctgga cagtggctgt ttttacaggg atgcgacacg
     3901 gtgaaattgc cgcacttgca tgggaggata tcgacctgaa agctggcacg ataacagtgc
     3961 gacgaaattt tacaaaaata ggtgatttta cgctaccaaa gaccgacgca ggcactaacc
     4021 gggttataca tcttctggca ccagcaattg aagcacttaa aaaccaggcg atgcttactc
     4081 gtcttagcag gcagcatcag atcactgttc aattacgcga gtacggaaga acaattttgc
     4141 acgagtgcac ttttgttttc tgtccgcaaa tcgttcgcaa gaatcacaag gcgggtatta
     4201 actacgcggt aagctccatc ggagcgacat gggattcagc aataaaaaga gcgggtatcc
     4261 gatcccgtaa agcgtatcag tcacgccata cctatgcgtg ctgggcttta tcttccggag
     4321 caaacccgac atttattgca tcacagatgg ggcactccag cgccagcatg gtctacaatg
     4381 tttatggtgc atggatgcct gagtgcagcg tgactcaagt tgccatgttg aataatgtcc
     4441 ttaatgcccg tgccccagac gtgccccaaa gtgaccagga ggatgaaata aaattatatt
     4501 tttcaaaatg ataaaccata tccttagaca tgttgaaacg atggcacgcg cagtcgctga
     4561 gggtgcaagc aaagtggatg gcgctgaagt tgtcgttaag cgtgtaccgg aaaccatgtc
     4621 gccgcaatta tttgaaaaag caggcggtaa aacgcaaact gcaccggttg caaccccgca
     4681 agaactggcc aattacgacg ccattatttt tggtacacct acccgctttg gcaacatgtc
     4741 cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg tgggcttccg gcgcactata
     4801 cggaaaactg gcgagcgtct ttagttccac cggtactggc ggcggtcagg aacaaaccat
     4861 cacatccacc tggacgaccc ttgcgcatca cggcatggtg attgtcccca ttggctacgc
     4921 agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt acgccgtacg gcgcaaccac
     4981 catcgcaggc ggtgacggtt cacgccagcc cagccaggaa gaactgtcta ttgctcgtta
     5041 tcaaggggaa tatgtcgcag gtctggcagt taaacttaat ggctaatctt caacaggagg
     5101 atacgcatgc caactcaaga agcgaaagct catcacgtcg gtgaatgggc atctctgcgc
     5161 aatacgtcgc cggaaatagc cgaggccatt ttcgaagtcg ccgggtacga cgaaaaaatg
     5221 gcggaaaaga tttgggaaga aggtagcgat gaagtgttag tcaaagcctt tgctaagacc
     5281 gacaaagatt cgcttttttg gggcgaacag accatcgaac gtaaaaacgt ttaaccagcg
     5341 actcccccgt agcgaacggg ggagttttct gttatttcac cgcttcattc aacacgctat
     5401 caaacttatc catcgggcag aaaccatcag cgtctatcgg gcaaccgctt aattccagcg
     5461 tcacgcgctg cgcaggtgcc tgcagggtta acgcatcggc attacgtaac tgttccgcac
     5521 tctggtacac atattcaatt ttcatcaaat cgcgattggc tttgctgtca cgccaacgct
     5581 ggaaaacgat tttgccgcca atcggcgtgc gttcgttctg gtcatgcaac tgatacggtt
     5641 tgaaatccag cgccgttaac agagaggcaa tgttggagtc atgcccaact aacaccgtga
     5701 tttttggagc actggtgcga tcggtgacca gggctttgtc gatataactg accagcggtt
     5761 tcgcaacatt gcgcgccact tccggtgagg taaacaggct gtcctggtaa ccgtttttca
     5821 gcttcgacaa caccttccac tgctggtcag atttgatttc tccccaggcc acctgatcca
     5881 tcggaaaacc ttcgtagtat tgcaaagtaa acgcatctac cagcgagttg ccgactttca
     5941 gcggcccgga aacacctggt tcttgttgat acttggcgct aaaggtattt ttgccatcca
     6001 ccagcgaaca ctgttgtttc tctttacagg caggggaatc tttatagtta acgatttttt
     6061 ccagtagctg gtagctgtcg gtaagctgga gtttgctgag ctctttctcc attgccgcca
     6121 ccgcctgttc actgaatgcg gcggaatcat cggtgatcac cgggttaaag gttgggtcca
     6181 tagtgcccat tttttcctgg tgatgcacag gaatatcaca ccccgggaat gcgccggtaa
     6241 taaagaactg tgcggtcgca acggtacgtt gcagactatt ggcataggca taaacggtgt
     6301 ccggcggcgg gcattccccc gattttacca tcccctgctc tgccagccat tcacgcatgt
     6361 aatggcccat atacacttcg agtacgccac ctttggtggt gagttgccca ccggggacgt
     6421 cccattcggg ccatttattc ggcgtcgact gctccagcac actgccattg ttcgccagcg
     6481 gcgcacgtaa gttatggcgg ctcatcatga gcacttgctg tagctgatag ccttccggta
     6541 cggtttgtgc ctgagcgttt gaagcgagta aaactacccc tgccacagct gcggcgatta
     6601 gcgttttgtt cattcctggc acctcttttg ttatcaatca ctaaagagtg tgacagaaat
     6661 atgacttttt tccgggaaca ggttcgcaaa atggcttgtt gaacggctgt ttttatattc
     6721 atctttaaaa acaggcaagg tttgacgcaa actatgcctg attagcccct gcctcgcgta
     6781 cgccgctgat gattgcacct gtggctaaac agaatatcgg cggcgttacc aatacagagg
     6841 caatatttac caataaaaaa gtgtgagact aataacaatg gtttttagca tatgcctctg
     6901 tcattaaatt gcataacgag ttaaaaaacc aatttgcgca ttcctttcct tattgtttaa
     6961 ctcgtaagcc tgagcaaaaa gttcaggagg gaggtaaacg tcataatgat tgccagtgtt
     7021 tattaaataa ccatactcaa gatcttcatc tgttgaaaag gattcattcc ctaaggagat
     7081 tgatgccaca ttactattaa caataaataa tgagcaaatg ctatctgaaa gagcagtgag
     7141 gaaatttttc acctcat
//
LOCUS       DAZFKY010000213         7155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFKY010000213 DAZFKY010000000
VERSION     DAZFKY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..378)
                     /locus_tag="ACHKCL_004186"
     CDS             complement(<1..378)
                     /locus_tag="ACHKCL_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5765040.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENL"
     gene            complement(391..2172)
                     /gene="gcl"
                     /locus_tag="ACHKCL_004187"
     CDS             complement(391..2172)
                     /gene="gcl"
                     /locus_tag="ACHKCL_004187"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5765041.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2262..3077)
                     /gene="allR"
                     /locus_tag="ACHKCL_004188"
     CDS             complement(2262..3077)
                     /gene="allR"
                     /locus_tag="ACHKCL_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5765042.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3155..3637)
                     /gene="allA"
                     /locus_tag="ACHKCL_004189"
     CDS             complement(3155..3637)
                     /gene="allA"
                     /locus_tag="ACHKCL_004189"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5765043.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3867..4793
                     /gene="allS"
                     /locus_tag="ACHKCL_004190"
     CDS             3867..4793
                     /gene="allS"
                     /locus_tag="ACHKCL_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5765044.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4862..5956
                     /gene="mnmH"
                     /locus_tag="ACHKCL_004191"
     CDS             4862..5956
                     /gene="mnmH"
                     /locus_tag="ACHKCL_004191"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5765045.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            6072..6437
                     /gene="ylbG"
                     /locus_tag="ACHKCL_004192"
                     /pseudo
     CDS             6072..6437
                     /gene="ylbG"
                     /locus_tag="ACHKCL_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1814 a         1877 c         1728 g         1736 t
ORIGIN      
        1 caggttttct acaagcgttg cgtgaatctg ttcactgctg aaaccagccg gcgttttacc
       61 gaccagacag ttaatatttt tattacccag cgcacgggca taacgaatcg ctgctgctac
      121 gccatcccga aactcttctt cacggccagg aatacaggca ataccgcgct cccccgccgc
      181 ccagtcaccc gccggtaaat tgtgcagcgt atgttcgagt ttattactcg ccagcacctg
      241 ttttaattct tcaatgtcgt agtcataagg aaacataaat tcaacgccgc gaaaaccaca
      301 ctgcgcagct ttctcaaaac gggcgagaaa atcatattca ccaaataaca tcgataaatt
      361 agcagagaaa cgtaacatta tttatctccc ttattcatag tgcatgaagc aggtttcagt
      421 cggtgcatcc gctgcgttat cggcgatatc ttcaaattcc atgacgttat ccagttcgct
      481 gcccatcgaa atatttgtca cacgctcgag aataacttcc acgactaccg gtacccgata
      541 ttgcgccatt aaggctttcg cctgttcaaa ggccggtgca atatcttccg gtttgaagac
      601 gcgaatagct ttacaaccta aaccttccgc tacttttacg tggtcgacgc cgtaaccgtt
      661 cacttcgctg gagttgatat tctcgaaagc gagttgcacg cagtagtcca tgtcaaaggc
      721 gcgttgtgac tgacgaatca ggccgagata ggcgttattc accagcacat ggatgtacgg
      781 aatgttgaac tgcgcgccaa cggctaactc ttcaatcagg aactggaagt cgaagtcacc
      841 ggaaatcgcc accacattgc gtttcggatc agcggcacaa acccccagcg ccgccggaat
      901 cgtccagcct aacggaccag cctgaccaca gttgatccag tggcggtctt taaagacatg
      961 cagcatttgt gccgcagcga tttgtgacag gccaatggtg gtgacataac aaacatcgcg
     1021 accaaaggct ttgttcatct cttcatacac gcgctgcggt ttcaccggca cgttgtcgaa
     1081 atgggttttg cgcagcaaag tgcgtttgcg ctgctggcaa tcggcgaccc attctttacg
     1141 acacggcaga cgacccgctt tttgcatctc ctgcgccact tcaaccagca gtgtcagcgc
     1201 cgctttagca tcagagacaa tgccgagatc cggacacagc acgcggccaa tttgcgtcgg
     1261 ctcaatatcg atatgaacga ttttgcgccc ttcggtgtat ttctctaccg aaccggtatg
     1321 acggttagca aaacggttac cgataccaaa caccatgtcg gacgccagca acgttgcgtt
     1381 accgtaacga tgcgcggttt gcagaccgac catcccggcc atcagttcat gatcgtccgg
     1441 gatacagccc cagcccatca gcgttgggat caccggaacg ctggtcagtt cagcaaactg
     1501 ttgtaacagt gcagctgcgt cagcattgat taccccgccc ccggcaacaa tcaccggacg
     1561 ttcggcctgg attaacattt ctacagcttt ttcgatctgc atacggctgg cagcaggttt
     1621 gtagaccggc agcggttcgt acatgtcagg atcaaactcg atttccgcaa cctgaacgtc
     1681 gaacggtaaa tccaccagta ccggacccgg acgaccagaa cgcatcaggt gaaatgcctg
     1741 ttgcagcacg cgaggcacca gcgccgcttc acgaactgta accgccattt tgctgaccgg
     1801 tttagcaatt gcttcaatat ctacggcctg aaaatcttct ttatgcagac gggcgcgcgg
     1861 tgcctggccg gtaatgcaca gaataggaat ggaatcagca gaagcggaat agagcgcggt
     1921 gatcatgtcc gtgcccgcag gaccggaagt ccccagacat acgccgatat tccctgccgt
     1981 tgcgcgggta taaccttccg ccatgtgcga agcaccttcc acatgacgcg ccagaatgtg
     2041 acgaataccg ccgtgcttac gcatcgctga gtagaacgga ttgattgcag ctcccggaac
     2101 gccgaaggcg gtggtgatac cttctttctc cagcacatac attgccgcgt caacggctct
     2161 catttttgcc attttattcc tacccccatt aattggaaaa attttccaac tttcaaattt
     2221 tgtcaaaaaa acccgctccg cagagcggga tgcgacagac actatggatg tgctttcagt
     2281 cccaacgccg tgctgatatc gcgggcggtg tctctgacca gctcaccctg actgacaaaa
     2341 cgatcttctg tcagtcttga tgaaggcccg gagatggaga tagcggcaac aacgctacct
     2401 acatcatcgt aaattgctga agctatgcaa ttcagaccta caacatgctc ttctttatct
     2461 acggtatagc ccagttcacg cgcttgttcc aggtccttca gcagggtggg catatccaca
     2521 agcgtagttg gcgtaaactg ctgcaaaccg gtttgcagaa tgatgctcat caactcctct
     2581 tccgccagcg gataaagcag cgctttgccc gcaccggaag catgcagtgg cagacgactg
     2641 cccagtggcg cgcacatcct gaccatcgat ttacactcta actgaccaat taataccgct
     2701 tcattgccgt tacggatcgc gacattgacc gtttcgccgg aaagtaacat caggcggcgc
     2761 ataaacggcc cggcgacgga gaggacatcg cggttatgga tgtacgccgc accgacgtta
     2821 aagacaccta atcctatatg ccaccagcct aactgactgt cctgatagac aaaatccgct
     2881 gcctgtaaaa ccttcagcaa gcgaaaggtc gtggagagcg gcaaatccag attgagagaa
     2941 atatcgctaa ccgacgaact tcccccactt ttttccagat attgcagaat cgcaattccc
     3001 cgctctaatg cctgtgcgcc cttctgtgcc acaggctccg cctgtcctgg cctgccgcgc
     3061 cgtctaactt ccgtcatacc tacgttaact ccgacctaca agtgagcata aacaatgaac
     3121 aattagttta tcttatgcaa gtcggttgca ggcgtcacgc aaaacagagt tcctgttcag
     3181 gaatactttc aacatcacag ttgtcactgc cgccgcgatc gatggtcaga aaatcggtga
     3241 cgcgctgcca ggcgaaaagt gggtgatgcc agacgttacg atggtaattc actccctgtt
     3301 cgccgttggt aataaacgcc cgcagggttg acaggtctgg tttgtcgtca cctaacgcta
     3361 cgaccaccac aaacacctca cctttcatcg ggataaaggc ctgagtaccc agcggatgac
     3421 gttcgagttc gtgaatggtc agcggcagat tcgccggttg cgctcggtta atgctgataa
     3481 gcgtacggtc ttgctcaaga atctcaacca gcgccaaatc gtggtaacgc tccaccaggc
     3541 cattgttaat atggaaaaaa tcccgttgct gcgtttcgat tacgtcgcca taagcactaa
     3601 aggcttcctg acttaacggt aatacctgaa gtttcatctt tttgtccttg taacataatt
     3661 ccgttcaacc agcgccgacc ggtcagcttg gtttcaataa caggcaatct aaaaccttaa
     3721 gagaacaaca tcaacacaaa acttggaata tttttccaaa actagattta gtccacaaat
     3781 tgaacagggt tatcatgtaa aaatactgat aaatcagagg ataagaaaat cacgcaactt
     3841 gcggatttaa aaggggatac ctgaagatgt tcgatccaga aaccttgcgg actttcattg
     3901 cggttgctga aacaggaagt ttttcaaaag cggcagaacg attatgtaaa accacggcga
     3961 cgatcagtta tcgcattaaa cttctggaag agaataccgg agtggcgctg ttttttcgca
     4021 cgactcgtag cgtaacgttg acagcggctg gcgagcatct actttcccag gccagagact
     4081 ggctgagctg gctggaaagt atgcccagcg agctgcaaca ggtgaatgat ggcgtggaac
     4141 gccaggtgaa tattgtcatc aacaacctgc tctacaaccc ccaggccgtc gcccagttgc
     4201 tggcgtggct gaatgaacgt taccccttta cccagtttca catctcccga caaatctata
     4261 tgggcgtctg ggactcgcta ttgtacgaag gtttttcact ggctatcggc gtcacgggaa
     4321 ccgaagcgct ggcaaatacc tttagtcttg atcccttagg atcggtgcaa tggcgatttg
     4381 tcatggcggc ggatcatccg ctggcgaacg ttgaagagcc gctaacagaa gcgcagttgc
     4441 ggcgctttcc ggcggtcaat attgaagaca gcgcccgcac cttaaccaaa cgcgtcgcct
     4501 ggcgattgcc agggcaaaaa gagattattg ttcctgatat ggaaacgaaa atcgccgccc
     4561 atctggcggg cgttggcatt ggttttttgc caaaatcgct ttgccagtca atgatcgata
     4621 atcaacaact ggtcagccgg gtaatcccaa cgatgcgccc tccttcgcca ttgagtctgg
     4681 catggcgcaa atttggcagc ggcaaagcgg tagaagatat tgtgaccttg tttacccagc
     4741 gcaggccgga aatcagcgga tttttagaaa ttttcggcaa cccacgcagt taaaaatcaa
     4801 gtttccttgc gcgttggcgc gtaaaatagc gcgcaagctg atttgaggtt cacttttaac
     4861 tatgcaagag agacacacgg aacaggacta tcgtgccctg ctgattgctg atacgcccat
     4921 tattgatgtt cgcgccccta tcgagtttga gcaaggcgca atgcccgccg ctatcaatct
     4981 gccgttaatg aataacgatg aacgcgccgc cgttggcacc tgctataaac agcaaggctc
     5041 agacgcagcg ctggcgctgg gacataaact ggttgcgggt gaaattcgtc agcagcgcat
     5101 ggacgcctgg cgggcagcct gcctgcaaaa tccgcaaggt attctctgct gcgcccgtgg
     5161 cggtcagcgc tcacatattg tgcaaagctg gttgcatgca gcggggattg attatccgct
     5221 ggtggaaggc ggttataagg cactgcgcca gaccgcgatt caggcgacta ttgaactggc
     5281 acaaaaaccg atagtgctga ttggcggttg taccggcagc ggtaaaacgc tgttagtgca
     5341 gcaacagccg aacggtgttg atctggaagg gttggcgcgt catcgcggtt cggcgtttgg
     5401 tcgcacgtta caaccacaac ttagccaggc gagttttgaa aacctgctgg ctgccgaaat
     5461 gctaaaaacc gacgcccgtc agaatttgcg cctgtgggtg ctggaagacg aaagccggat
     5521 gatcggttcg aatcacctgc cggaatgcct gcgcgagcga atgactcagg cggcgattgc
     5581 ggtggtagaa gatccgtttg agatccgtct tgagcgcctg aacgaagagt atttcttgcg
     5641 tatgcatcat gattttaccc acgcgtacgg cgacgaacag ggctggcagg aatattgcga
     5701 atacctgcat cacggacttt cggctattaa gcgtcggctg gggctacagc gatataacga
     5761 actggctgca aggctggatg cagcactgac aacgcaactc accaccggca gcaccgacgg
     5821 tcatctggcc tggctggtgc cgttacttga agaatattac gacccgatgt atcgctatca
     5881 gcttgagaaa aaagcggaaa aagttgtctt tcgcggtgag tgggcggaag tggcggaatg
     5941 ggttaaggcg cagtaatgag ggacgtattt gtattagtcg cgacttgatc tgacacatgg
     6001 ccttgaaagg ttgagagtta ccggttttga tatgagtgtc gaatccttat ccaaaacaag
     6061 aggtaactct catgcttcat actgccaatc ccgtcatcaa acacaaagcc ggtttgctca
     6121 atctggctga agaactcagc aacgtatcaa aagcctgtaa aatcatgggc gtttcgcgtg
     6181 atacgtttta tcgttaccgc gaactggtcg ctgaaggcgg tgtggatgcg cagattaatc
     6241 gtagtcgccg cgctcctaac cttaagaacc gtaccgatga ggcaactgaa caggctgttg
     6301 ttgattacgc cgtcgctttc ccgacacatg gtcagcaccg ggccagcaac gaactgcgta
     6361 aacagggggt ttttatctcc gacagtggtg tccgttctgt ctggttactc cataaccttg
     6421 agaatctcaa aagacgatac tgaacaaatt ttatcaggtg acgttccgta aaaagttgta
     6481 tggagttctc gatgcagtcg gcgcgactat cagtaccccc gctgaaccct ggactatcgg
     6541 gccgccgtac tggggcatgt caccctggcg tgccgctggt tttccgctaa tggtttattt
     6601 cctctttctt tcccggcaag aatttatttt aatccggaag gattatttat tttcagatag
     6661 ggactcacat taaacacgta acattttaat taatgcatgg ctgaatatgt attacacaat
     6721 gtcagatatc gtaaaagacc agtgcaattc tatgtaaact ctctgactgt tcattttatt
     6781 tgttgtttca gggtcggaac tcaccctcaa taaacaactg aatacgagta gatattcatc
     6841 gtctgagcta tatggcttta cacaatagcc gacatgtctt ttctcagtca ggaattttac
     6901 ttttcctcac tacgaagttg acgacttcga tatgggatag actcttaatt caagcaatta
     6961 ctctgcacaa gcatcaggca atccagctcc aatatttgtt ataaaataat gcgtaacatt
     7021 aatttcacca acaaaaaaag gtttacacca tccccttgcc ctggtatctt accatattta
     7081 caccacactt tttttgtatc tggatcattc tttgatacga ttttcgccac ttgttccctg
     7141 gccctcactg tcaat
//
LOCUS       DAZFKY010000214         7135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFKY010000214 DAZFKY010000000
VERSION     DAZFKY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(311..1447)
                     /locus_tag="ACHKCL_004193"
     CDS             complement(311..1447)
                     /locus_tag="ACHKCL_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5765046.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1450..1812)
                     /locus_tag="ACHKCL_004194"
     CDS             complement(1450..1812)
                     /locus_tag="ACHKCL_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5765047.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2349..4262
                     /gene="mtlA"
                     /locus_tag="ACHKCL_004195"
     CDS             2349..4262
                     /gene="mtlA"
                     /locus_tag="ACHKCL_004195"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5765048.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4357..5505
                     /gene="mtlD"
                     /locus_tag="ACHKCL_004196"
     CDS             4357..5505
                     /gene="mtlD"
                     /locus_tag="ACHKCL_004196"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5765049.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5505..6092
                     /gene="mtlR"
                     /locus_tag="ACHKCL_004197"
     CDS             5505..6092
                     /gene="mtlR"
                     /locus_tag="ACHKCL_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5765050.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6104..6313)
                     /gene="yibT"
                     /locus_tag="ACHKCL_004198"
     CDS             complement(6104..6313)
                     /gene="yibT"
                     /locus_tag="ACHKCL_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5765051.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6598..6960
                     /locus_tag="ACHKCL_004199"
     CDS             6598..6960
                     /locus_tag="ACHKCL_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5765052.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1821 a         1790 c         1804 g         1720 t
ORIGIN      
        1 atatttttta aatgtgactg tcatccttca gataataaag ccatcatcgc aatagatgga
       61 aaagcactcc tgctcttttt tgattagagc cgtcgcagga ggaacaactc atatcatcat
      121 tgcgatctcg acgatgtata gtctcatcct gcggcagaac aagacggata aaaaatccaa
      181 tgagagcaca ctcatccaga acttattaac ataatgaata ttaaagaaaa atcatcgcag
      241 ctaatgaagt aatatgcaaa aataattagt ccttacaagg agcaaagttc cataatctcc
      301 cccctccccc tcaatgatcc aaataaagat aatgcatcca gctggtcatt cttagcagca
      361 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aaccgccacc tgggcgtaga
      421 tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacac
      481 cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
      541 ctggcacgac aggtaaaata ctagtcagtt tgccgtgaaa cacctgccca ggtagcgcgt
      601 taaacaccac ttccgcatca tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt
      661 gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg
      721 cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg ctcggcgcgc
      781 gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa
      841 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
      901 ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
      961 cacgttccga gaacggattc accgccgcct ggctgccttt caagtaacgt tgataatttt
     1021 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct
     1081 gcgcacgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa
     1141 ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct
     1201 gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg
     1261 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aataaaatca
     1321 aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac tgatttaccg
     1381 gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa acaatcaata
     1441 gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg
     1501 atgctccagc cgggaaattc ggtcagaaag tgaggcgatt tccgggtcag tcgctttctc
     1561 ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca
     1621 gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg
     1681 aatagcgtcg gcatgggggt gattgcgctt tttggcaatc agataaggga tgtcatgtat
     1741 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
     1801 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgtttttgg gctggcagcc
     1861 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
     1921 caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt atgacgaagg
     1981 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
     2041 cacaaagatt caacaaacca tcaaaacaaa aatgtgacac tactcacatt taaatgccat
     2101 ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cgttttgtga tgaacgtcac
     2161 gtcaattacc tctctacccc ctatatttat gtgattgata tcacacaaaa ggccgtcgac
     2221 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
     2281 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
     2341 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
     2401 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
     2461 ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt
     2521 atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc gaacgtggcg
     2581 gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
     2641 tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
     2701 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
     2761 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaaaccctgt
     2821 ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
     2881 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
     2941 tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc ttcctgattg
     3001 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta
     3061 gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa
     3121 tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc ctcggcggta
     3181 tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg gcatctccgg
     3241 gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct aacatcgcgg
     3301 gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg aaaaccagca
     3361 aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg
     3421 agtctaaagg tgcatctccg ctgtctgcgg gcgatgtgac taacgacctg agccacgtac
     3481 gtaaaatcat cgttgcctgt gacgccggta tgggttccag tgcgatgggc gcaggcgtgc
     3541 tgcgtaagaa aattcaggat gcaggtctgt cacagatttc ggtcactaac agcgcgatca
     3601 acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc gaacgcgcta
     3661 tgcgccaggt tcctcaggca cagcatattt cgctaaccaa cttcctcgac agcggcctgt
     3721 acaccagcct gactgaacgt ctggttgctg cccagcgaca tactgaaaac gaagtgaaag
     3781 tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg
     3841 cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt cgttttgctg
     3901 gcgagcagct ggtgaaaggc ggttacgttg agccggagta cgttcaggcg atgctggatc
     3961 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
     4021 aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc
     4081 gcttcggtga agaagaagat gacattgctc gtctggtgat tggtattgct gcccgtaaca
     4141 acgagcacat tcaggttatc accagcctga ccaacgcact ggatgatgag tccgtcatcg
     4201 agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca ggtcgtaagt
     4261 aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag
     4321 ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca
     4381 ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg
     4441 tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt
     4501 catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcgatgc tgtcagcagc
     4561 attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc
     4621 ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgtaaagaa
     4681 caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg cggtaccacg
     4741 cagctgaaag gtcatgtgat gaacgcccta ccagaagacg ccaaagcgtg ggtagaagaa
     4801 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
     4861 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
     4921 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
     4981 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
     5041 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
     5101 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
     5161 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
     5221 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
     5281 aagccattgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
     5341 gctggtgcaa tgcacttccg cagtgaagac gatccgcagg ctcaggaact ggcagcactg
     5401 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccgatcttga tgccaacagc
     5461 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
     5521 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
     5581 aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
     5641 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
     5701 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
     5761 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
     5821 aatacgaaga tgcggagctg ctgatggcat tgcgtgaaga actaaatcac gacggcaacg
     5881 agtacgcctt taccgacgac gagatccttg gaccctttgg ggaactgcac tgcgtggcgg
     5941 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
     6001 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
     6061 gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
     6121 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
     6181 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
     6241 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
     6301 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
     6361 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
     6421 atccgctgct gtttattatc ttttttctat agatttctta attaatcaat aaattgcgta
     6481 gtttttaagc atgttaatga ttacgttaca gaactattgc aatgtcatca gttgtgaaca
     6541 aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag ccatcacatg
     6601 aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc gtctggacgc catccgccac
     6661 cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct ggaaaaagag
     6721 aaagccacgc tggaagcaga aattgctcgc ctgcgtgaag ttcatagcca aaaactgagt
     6781 aaagaagcac agaagctgat gaagatgccg tttcagcgcg caattaccaa aaaagagcag
     6841 gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc
     6901 gcgctgggcc gtgaaatggg cctgcaggag atgactggat tctctaagac tgcgttttaa
     6961 gcgcctcaga cgactggttc gatcatatca acccgcatct ggcttgcctt agctggtcaa
     7021 tgcggggact ctcctgtcta ctcgcctaat taccaaacaa ttcactccct ttacgtcgat
     7081 ttaccgctgg ttgatattct gaaaatgcat ttttaccgaa atagccacct ataaa
//
LOCUS       DAZFKY010000215         7109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFKY010000215 DAZFKY010000000
VERSION     DAZFKY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..179
                     /locus_tag="ACHKCL_004200"
     CDS             <1..179
                     /locus_tag="ACHKCL_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765053.1"
                     /translation="RILHTLLKKDVKYISHYKISVFNKFEIACDSDGFYIFKALIQLR
                     RFTGFKNVRLYQLH"
     gene            222..782
                     /locus_tag="ACHKCL_004201"
     CDS             222..782
                     /locus_tag="ACHKCL_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5765054.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            868..1569
                     /locus_tag="ACHKCL_004202"
     CDS             868..1569
                     /locus_tag="ACHKCL_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5765055.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1622..4144
                     /locus_tag="ACHKCL_004203"
     CDS             1622..4144
                     /locus_tag="ACHKCL_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5765056.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4160..5494
                     /locus_tag="ACHKCL_004204"
                     /pseudo
     CDS             4160..5494
                     /locus_tag="ACHKCL_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5491..6180
                     /locus_tag="ACHKCL_004205"
     CDS             5491..6180
                     /locus_tag="ACHKCL_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5765057.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6326..7108)
                     /locus_tag="ACHKCL_004206"
     CDS             complement(6326..7108)
                     /locus_tag="ACHKCL_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5765058.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1917 a         1739 c         1661 g         1792 t
ORIGIN      
        1 aaagaatatt gcacaccctg ctaaaaaagg atgtcaagta tattagtcac tataaaatat
       61 cagtttttaa caagtttgaa attgcttgtg atagtgatgg cttttacatt ttcaaggcac
      121 ttatacagtt aaggagattt accggtttta aaaacgtcag attgtatcaa ttgcattaat
      181 taactcgttc tatattaact ctaaatttca aggatattaa tatgttttct actttcaaaa
      241 aagcagctct gctggcagct attgcattac ctttttcaac tatggctgcg cctacagtca
      301 cttttcaggg tgaagtaacc gatcagacct gttccgtaaa tatcaacggt caaaccaatt
      361 cagtagtatt gatgccgacc gtagccatgg ctgacttcgg tgcaacttta gctgatggtc
      421 agagcgcagg ccagacgccg tttacggttt ctgtgtctaa ctgccaggct ccaactggtg
      481 cagatcaggc aatcaacacc accttcctgg gctacgacgt tgacgctagc acgggtgtta
      541 tgggaaaccg tgataccagc agcgatgcgg cgaaaggctt tggcattcag ttaatggatt
      601 ccagcacttc tggtaaccca gtaactctgg ctggcgcgac taacgtaccg ggtctgaccc
      661 tgaaagttgg cgataccgaa gccagctacg acttcggtgc gcgttacttc gttatcgata
      721 gcgctgctgc cactgccggt aaaattaccg ctgtcgcaga atacaccctg agctacctct
      781 aattaatcta ctggctggca gtaaatgcca gccagtattt cttcgcacaa ttaatagtga
      841 tttttcatgg acatggaaaa gataagaatg aacatttttg catatttact ggtacttgta
      901 ttttccatga gcatgagcag cagcgcgttt gccagcgtgg taatgaccgg aacccgtatt
      961 attttccctg gtgacgcaaa ggaaaaaacc atccagttgc gaaataccag cgatcagccc
     1021 tatatcatta atatccatgt tgaggatgaa cgtggttctg acaagaatgt accgtttatg
     1081 ccaaccccgc agacatttcg catggaagct gccgcaggtc aggcgttacg cctgctctac
     1141 actggtaata atttaccgca ggatcgcgag tctgttttct ggtttagttt cagtcaacta
     1201 ccttatctga ataagaatga taaaagtcag aaccagctca tcctggccct gactaatcga
     1261 gtcaaaattt tctatcgtcc cagctcgatt gtcggtaaat ccagtgacgc acccaaaaac
     1321 ctgacttacc aggtaaaaca gaaccgcatt gaagtgacga atcccacggg ctattacgtc
     1381 acaattcgcg ccgctgaact gcttaataat ggtaaaaaag tccccctcgc gaattcggta
     1441 atgattgctc ctcaaagcac aactgaatgg acactaccct ctggcatcag tgtcgctccc
     1501 ggtgcgcaga tccatttagt gaccgtcaac gactatggcg taaatgttac gtctgagcat
     1561 gccttataac ctctttctcc ttttttgagc ggtatccttt ttcacgcacg agtacgtcga
     1621 gatgaataaa tccgttgtgt caatttctgc ggcaatgttg gttttacttt gccaaccggt
     1681 catggggagc gaaatctcac ccgcaacacc gtcagatgaa gacaactaca cctttgaccc
     1741 gcaactcttc cgcggcagca gatttagtca gtcgtcatta gcaaaactga caacacgtga
     1801 gtctgttgca ccgggcaatt ataaaatgga tatctacacc aacaataagt tgtcaggcag
     1861 ttggaatgtc acgtttaaag aagccgctga tggtcgcgtt ctgccctgcc tgacgcctga
     1921 agtcgcggac gcgataggcc tcaaaacagg ggaagataag ggggaaaaag atcctgtctg
     1981 tacgtttgct aaggaactcg ctcccggcat caccagccag acacagttgt cacaattgcg
     2041 cctggactta tcggtgccac agagtcaatt gattagtcgc cctcgcggct atgttccccc
     2101 cagcgagctg gataccggag catcgctggc gttcatgaat tatattgcca actattacaa
     2161 cgttgcctat tcagggcaga atgctcatag ccagcgttcg ctatgggcat catttaatgg
     2221 tggcatcaac cttggtgcct ggcaatatcg tcagttatcc aacatgacct gggataatga
     2281 caaagggaat cagtggaaca atattcgtag ctatttgcaa cgcccgctgc ccgccataaa
     2341 tagccagtta atgatggggc agcttatcac cagcggaaga tttttctctg gactcagtta
     2401 tcacggcgtt agtctcgcga ccgatgaacg tatgctgccg gactccatgc gcggctatgc
     2461 gccgactatt cgcggcgtgg ccgcaacaaa cgccagagtc tcggtaatgc aaaacggtca
     2521 tgaaatatat cagaccaccg tggctcctgg ccctttcgag ataaacgacc tataccccac
     2581 cagctacagc ggcgatctgg atgtcaccgt tacggaagct aacggcgcag tcagtcgttt
     2641 cagtgtcccc ttttcagccg taccagaatc gatgcgtcca ggaacttccc gttataacgt
     2701 ggaagtaggt aaaacgcagg atagtggtga tgactcgatg tttggtgacc ttacctggca
     2761 gcacgggatg actaatacgc tgacatttaa cagtggttcg cgtatcgctg atggctacca
     2821 ggcgctgatg ctgggcggag tctatggcag ttcgctgggg gcatttgggg caaacctcac
     2881 ttggtcccat gcgcgtgttc ccgaaagcga agcgcagagt ggttggatgt cgcaattaac
     2941 ctggagtaaa actttccagc ctacttcaac caccgtctcc ctggcaggtt atcgatactc
     3001 taccagcggc tatcgtgatc tggctgatgt gctgggagag cgtcatgctg ccagcaataa
     3061 acagtcatgg gactccagcc agtggcgtca acagtcgcgc ttcgatctta cgttaagtca
     3121 gagccttgcg aattacggca acctgtttgt gtcaggttca acacagaact accgtggcgg
     3181 caagagccgt gatacacagc ttcagttagg ttacagcaat agctttagcc atggcatcag
     3241 tatgaacctt tccgtcggac gccaaagaat gggcggctat aaagacaatt ctgatgatat
     3301 gcagacggta acatcccttt cattctcatt cccacttggc ggcaatggac ctcgtgtacc
     3361 aagtcttagc aacagctgga cccattcaac tgacggtagc tcgcaattac aaagctcgct
     3421 aaccggaatg cttgatgaag cacagaccac caactacagc ctgaacgtca tgcgcgatca
     3481 acaatataag cagacgacgc ttagcggaaa catgcaaaaa cgtttttcac aaactaccgt
     3541 cggattgaac gcatcgaagg gccaggatta ctggcaggct tcaggtaacg tacaaggcgc
     3601 gatggctgtg catggtggcg gcattacttt cggaccttat ctgggtgaaa cgttcgccct
     3661 ggtcgaagct aaaggcgcag aaggtgcaaa agtctataac tccagtcagc tggaaattaa
     3721 tgacagtggc tatgcgcttg ttccggcagt aacgccctat cgctacaacc gtatatctct
     3781 cgatccacaa ggaatggatg gcgatgccga gttggtcgac agtgaaagac aggtagcacc
     3841 ggttgcgggt gcggcggtga aagtaatttt ccgtacccgt cctggtaaag cgttgctgat
     3901 taaatcccgc atggcagatg gttcggaact gccaatggga gccgatgtgc tggatgagaa
     3961 taatacagtc gtcggtatag ccggtcaggg ggggcaaatt tacctccgca cagaacagac
     4021 aaaaggccac ttgtcagttc gctggggtga aggtgctaac gatagctgcc aattgccctt
     4081 tgatatcagc gggaaggaca gcaatagccc tatcatccgc ctgaatgaaa cctgtcagtc
     4141 ttgatacaga gaaaacccga tgtctaaatc gactttttta cacattctga ttagctccat
     4201 catattggtg gcactaatac aatcatccgc ttgggctaac tgtacaaaca cacagatagg
     4261 tcaaactgaa gatggtcgga cagcgcttat tgagttcgga aaaattaata tgaccgacac
     4321 ctattttgcg ccagcaggtt cactcctcgc gacaacggtc gtacctccca ctaactacac
     4381 atcaggtggc gcgacaggaa gctccgtatt gtgggaatgt gatgcaacag atttgccaaa
     4441 catctatttt ctggtcgcta ccaatggcga cgaccgcgtg gggggctttt acgatgcagg
     4501 cgggcctgat ggtctgagtg atgtctatgc cacctagttc gcttttgtcg gtctcaagca
     4561 gaccatggcg ggcgtgacgc ttggtcgtta ctggaagaaa gtgcccatca ccagttatgc
     4621 cactcaggga actaaaatcc agattcgctt acaggatatc cctcctcttc atgctgagct
     4681 ttatcgcatc agtacgctac ctgatacatc agcaacaaca agttggtgcg gtaataataa
     4741 tacagatagt agtggagtcg gattcgcaaa accttccggt acaatctata actgtgttca
     4801 gcccaatgcc tatattcagc tttccggtac cagcggcatt ttatttggtc atgatgagcc
     4861 cggcgaagat agttctgttc attgggattt ctggggtgct gataatggtt ttggttacgg
     4921 aatgcgttcg gccaatcgac tctacaacaa tgccacctgc gttgcccgca gcgccacgcc
     4981 gttagtattg ctgccgacaa ttgcagaagc acaactgaat gcgggcatgg aaagtaccgg
     5041 taattttaat gtccgcgtcg agtgtagtaa ctcggttcaa tcagggatta gcgatactca
     5101 gacagcatta ggaatccagg tgtctgaagg tgcatataca gcggcgcaaa aactggggat
     5161 tatcaatagc aacggcggcg tcagcgccct ggtctctgat aattatgacg cagcagagat
     5221 ggcaaagggc gttgggatct acatttctaa cagtgctcac cccgatacgg cgatgacgct
     5281 ggttggtcaa ccgggcatcg cgaagttaac ccccggagga aatgcagcgg ggtggtatcc
     5341 tgtatttgaa ggggcaacat tagaaggtgc gactcacccc ggatactcca gctatagtta
     5401 ctcttttatc gcccggttga agaaactgcc aaatcagaca gtcagtgcgg gaaaagtgcg
     5461 ggcaacggct tatatattgg tgaaaatgca atgaaatccg ttttgctgct cttaaccatc
     5521 ttgttcgtac agtcagtgga agccggcatc atgccggctt cttcacggat tatttatcgc
     5581 gatggcgatc gggaaaagac actgatgctg gtcaatacca atgactatcc ggtactggtg
     5641 caaagttggg tagataatgg tgaaggttcc ccagaagctg cttctccccc ctttgtggtg
     5701 ctgccaccgg ttttccgcct ggctgcgaga cgaacccagg ggttgcggat tatctataat
     5761 catgacccac ttccccagga tcgcgaatct gcattctggt taaatcttta tgaaatgccg
     5821 ccagaaactc cccatcaaga tcccacacat ttaacgctgg caatgaatac gcaacttaaa
     5881 atcttttaca ggccaaaatc tgtaactatg acgccccaag aagctatgcg taaaataagt
     5941 tggcatctgg gtaacgacgg cagcagtagc tatattgagc ttaataaccc aacaccactg
     6001 aatatctctt tcacctccat cagcattggg gctacaaagg tgcagcaaga aagcgatatg
     6061 atgataaaac ccttcagtca acgacggtac tggttagcaa gtaataacgt tagtggtgtg
     6121 gttgcggcga catatctcga tgataatggt gcacagataa aacaacaatt agcgcaataa
     6181 tgctctgagt ggtatgtaaa ctcatcgcga tggttcaacg ctaacgaata accactatac
     6241 ccacctgaag taccggaagt ggagaaatga caaaatctct ccactccatt ttccagtcaa
     6301 aacacctcaa cgcagccaaa aatcgttaac gaattgaata tccaccatca atgactaata
     6361 gttgtccatt gacataatct gaagcggcac tggcgaggaa caccattgcg cccataagat
     6421 cctgtgccct tccccagcgt tgagccggta tacgcccttt tattaactct tttattttag
     6481 ggttattata aataatagcg gtcatttcac tcacataata tcctggggcg ataccattaa
     6541 tctgaatatt atattccgca aaatcatcgg catacgcttt ggtcagcccc gctaaagcat
     6601 gtttagtcgc cgcataccct ggcgaccaac gtcccccatg aaatgacaat acggaacaca
     6661 tattaataat tttccctttt ctttgcggga caaatatatt caatgcggcc tggctcatat
     6721 cgaatgcagc attcaggttg agattaatca tcggatccca gtcgtttcga ttgaaatcga
     6781 taggacgatt aatgttacac acgcccgcgt tattgaccag aatatctaac tgaccgaaac
     6841 gctccagaca cctattaata atggtgcatg atgcatcagg ttttgttatg tctattgtca
     6901 taaactcaac ttcaacgcct tcatgagtta aatgcttcaa atatctatta tcaggctctt
     6961 tgatgtcaac caacaataga ttagcccctg ctttggccag agccgagcat aagcctctcc
     7021 ccagctcacc cgcggcccca gttacgatgg ccaccttgtt atgtaatgaa aaaaaatcca
     7081 atgaaaaatc ttcaatagaa aagctcata
//
LOCUS       DAZFKY010000216         7089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFKY010000216 DAZFKY010000000
VERSION     DAZFKY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(109..2109)
                     /gene="yehQ"
                     /locus_tag="ACHKCL_004207"
                     /pseudo
     CDS             complement(109..2109)
                     /gene="yehQ"
                     /locus_tag="ACHKCL_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            complement(2106..3242)
                     /gene="yehP"
                     /locus_tag="ACHKCL_004208"
     CDS             complement(2106..3242)
                     /gene="yehP"
                     /locus_tag="ACHKCL_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5765059.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(3235..5514)
                     /gene="yehM"
                     /locus_tag="ACHKCL_004209"
                     /pseudo
     CDS             complement(3235..5514)
                     /gene="yehM"
                     /locus_tag="ACHKCL_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(5525..6613)
                     /gene="yehL"
                     /locus_tag="ACHKCL_004210"
     CDS             complement(5525..6613)
                     /gene="yehL"
                     /locus_tag="ACHKCL_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5765060.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1648 a         1938 c         1907 g         1596 t
ORIGIN      
        1 ctcctccggc agaagcagaa aaagcttatt atgcttccat cggaaacgat gatctggcag
       61 cctgagttca cagataaaac actctccagg aaacccgggg cggttcaatc cttgttgctg
      121 aagcaggggg aataattgtt cgcacagcaa aacaccgtta ttcattgctt ctacccgtgc
      181 ctcgctttct gtattacgaa attgtcccaa tacatgtgcc agccgataaa aacccaccgc
      241 ggtgaggtca ttcgccagca actctgcctg accaatagca ctctgttcct gatagcgcca
      301 gccgttatgg agcagttgaa taagtaatgc ctggcagcgc atcagcaact gatgagcagt
      361 agacggcaca ggcagaacgc tggcagaagg tagtgatgcc accacaggcg cagtttctgc
      421 gtccagcgcc caggcacgag tctttgtcat cattacctgt ggttccagtg tcaattgccc
      481 atcaacaaaa ctgacaaagc cagaaaccag acacacgggg tcgtctgttt gttgcaaaag
      541 cgccgccatg cgttcaacgg cataaggcgc gctggctgag gctggcagtg ataacgtcag
      601 cagattatct tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc ggctgctatc
      661 ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga cctgattaag
      721 aggccgtatg caggaggggg gacgctggcg taaatattcc cgcaaagcca caatgcccgg
      781 ctggcgtaac ggcgcgctca acattcgcca ggcatcaggc gacagcggca caacgctgct
      841 taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg ctgcttgtga
      901 aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac gcgtagctgc
      961 cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa tactgccggt
     1021 gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc agcaacgcat
     1081 acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc cagagccaac
     1141 gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg ccatctcttg
     1201 tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc ctgcatgata
     1261 gtggctggcg cgggcgtgga aggcatcaac gctggcgcgc agttgccgta gcgattcact
     1321 cacccagcgc cagttgcagc gttccgccgc ttgctgcgcg cggctgaaag cggcctcgta
     1381 gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt gctgaacata
     1441 ttgacgacac gcgttgcctt cttcgctggc aaacgggtcg tcagatgatg tgacgtgttc
     1501 gctgcgcatc tgccagatta aatgagtaaa ttccgcttgc tgagttttgg cctcgacgaa
     1561 ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat cacagcgggc
     1621 gaagcgaatg ctgctgcggg aataaaaacg cacatcactc atcggtaagc gggctgaggg
     1681 aatttcgcca ggcgcacaga acaactcaat ggtgatgcct ttagcgacca gcacctgtgc
     1741 acgtttgcgg gtggcatcgg gcagggtagc cagttcttcc agccagattg caggctgcca
     1801 ctcttcttct ttttccgtgg gccgggtggt ggcacaaagt cgctgataac ttaacaccag
     1861 catcacgcga tgacggcaca tgccgctagc cccgcaggtg cactgagcct ctttcagtgc
     1921 ctggccgttc gccagctggg tacggacacc gtcactgaag gtggcgatta aagcgccgtt
     1981 ctcatggctg atttccggga cgttgccatt ttccagttcc ttaaggctgc gcttaacaaa
     2041 accggcattg cttaacgccg tcagggcctg cggcgtgagt tctaataatt ccggacgtag
     2101 tgaattcatg actgcaaatt ctccgcaagc caggtagcca gttcacccgg tgtcattgcg
     2161 gctatttgtg cgccaacatt aaccagcgcc tgggccatat cgcggtcata gcaaggcgtt
     2221 gcggtgctgt cgagcgctgc cagccccagc actttgatgc cgctctggac acactttttc
     2281 acctgatgcg tcagcaatga tgatgaaccc ccttcataaa aatcactcac gaggataatg
     2341 acgctttttg ctggttgttc aataagttgc cgcccatact ccacggcact ggcgatattg
     2401 gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac gtctgccgtg
     2461 agatcaacga cactggtgtc aaacgccacc agatgggtac gaatgccggg taactgccac
     2521 aaacaggccg ccattaccgc agagtggatc accgaatcga ccatcgatcc gcttagatca
     2581 accagtaaga ccagttgcca ttgttcactg tggcgcttaa ttcggctgtt aaagcggggg
     2641 gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcagattggc gcgaagagta
     2701 cttttgaaat caaagtctcg cgccagtgga atagatgagc ggcgacggcg atcgcggaca
     2761 ccagaaaaag cctgacgaac ttcctttgcc agtcgagcca taatttcttc aacaacctgg
     2821 cgcactatcc ggcgggcggc agccagtact tcggggttca tcagatgttt ggtgtgcaaa
     2881 acggcgcgta gcaggctttc agaaggctgc atacgttcca gcacgtcgag attcgtcacc
     2941 acatcttcaa tgccgtagcg cagtacggca tcgctttcca gccgctcaat cacctgttgc
     3001 ggaaacagcg tgtgaatact gttgatccac tcaggggtgg tgagatttga gccacctaat
     3061 ccaccggagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag ccactccagc
     3121 gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc tgccgcttcg
     3181 ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt cagttcagac
     3241 atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt gttgatgatg
     3301 tgcaatggct tgtggtgggc aatgcaacgg catttgcagc gccgaaacgg gaagttgcgc
     3361 cagttgataa tgttcaagca cctgatgcgc cagtgtcccg cgttctcgtg gcggtagcca
     3421 ggccatcgcc gcgtgtaaat cgggcagggc attgataaaa tcggcatcac ttggttgatt
     3481 taaatgactg ctgaaaccgg cgataaatgc cggctgacag gccagttgat ggcgggccag
     3541 cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg ccagcatcgt
     3601 cagcgccgct tcggcactag cgttgggatg ttccagacgc atcagtgcgc caagagccgc
     3661 gccgcgatcc agcgccgggg catggattgc ctgactgcgt cgctccagta aggctaccgc
     3721 cgcagaaaga gaaacgccgg gtaaattaac gccgctatgt agatcgcgca gaatatggca
     3781 aagcgcttgc cagctatgca ggtgagcgtg aaactccttt tcatccggtc tgccgttaga
     3841 ttcacacagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgtg atatctgcgc
     3901 accttgcata ccgctaattt catccagccg ccataaggca tatagcactt ccagcgccgg
     3961 gcccatttcg gcaaattgat tttcctaggc gattaataat gtgagttgct ccagtaattg
     4021 ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg tgatactgcc
     4081 gattccaccc gcgtccagca tatcggcttc taatttatgg cgtgcggctt cctggagtgt
     4141 ggcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc gggttaattt
     4201 ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc gtacaatccc
     4261 tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc cattggggtt
     4321 aaagcgattt aaggtaagct ccgccgaaag agagattgcg gtacgttcca gttcgcaggt
     4381 gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga atccgtcacc
     4441 cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa taacgccgcg
     4501 atagctccac ggcaacggcg cgttcagggc ttctttaatc agcgagcctg ctatggcatc
     4561 cagccagtca gtgcgtaacg gtagtttata tccgcgcaac tgtgccagcg ccatcgcatg
     4621 cagatgagcg gcagccatat ccgcggtcga agcaggcaag tggtgttggc gcaaacgggt
     4681 aagaaccgtt tttagcagtt gttctcccgc ctgctgtaag ccccactgcc agcaccagct
     4741 ttgccagacc ggggcaggca ttcctgaaag gtatcctgcc agcacatcaa ggcgcttttc
     4801 actgtagggt gtgagataac aacctgtaac ggcatctgcc agcgagggca attctggctt
     4861 gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt gccagccacc
     4921 gcagacgact aacacgtcgc cattattttg ctgcactgcc catgcaatcc agcgagccat
     4981 aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca gttgggcaaa
     5041 atagtgcgcc agtgcagagg gtaatgcagt ttgctggctt tcatcttcga acaagtgatc
     5101 ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agcaaggcct ggctttcctc
     5161 ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca aatcgataaa
     5221 gtaagtttgt gcctgaatat gacgtgcggc ttgtagcgcc tgccactccg gcgaaaattc
     5281 ggcaaatggc gtccaggcgc cgcgcccagg agcagcaccg tcctgatact ggcaataact
     5341 gtaaatagcc accggaagct ggtgggctaa aaacagttca tctacccggt cattaaaatc
     5401 agccgggcct tcaatcaaca cgtatcgtgg tcgctggctt tcgattaacg atttcaccag
     5461 tcgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc tcatgcatct
     5521 ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct tctttatgtg
     5581 tggcgacacg ctgttcgaag taacggcgca gacgagcgcg atcttcttcg ttatctttga
     5641 cgatggttcc ggcaatacag tccaccaggt cggcaggctc ccccgcgcga ttcgctaaaa
     5701 accaggcgcg gacgcccaca gcgtgggcca cgttcacggc ttcagcggtg gacataatcg
     5761 ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg cggaaggtgc
     5821 ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta tgggccagca
     5881 aacgcgccga agcactggcg acaagctcca gctcctgggc gaaatccata atcggaaaga
     5941 cagtttcaaa atcgaagcga cgctttagcg cagcactcat ttcattgacg ccgcgatcgc
     6001 gggtattggc agtggcaata atattaaagc cttctcgcgc atagagctga ctggcttcac
     6061 cagtgagttc cggcaccgtc atcaccctgt cagaaagcat acctaacaga cagtcttgca
     6121 cttccagcgg tgtacgcgtt atctcttcaa aacggacgat tttgccgtcg cgcattccct
     6181 ggtaaagcgg ggcagggacc agcgcttccg ttgatggacc atgattgata agcagggcgt
     6241 agttccagcc gtacttgatt tgatcttcgg tagtagatgc gccgccctgg atggttaacc
     6301 cggcatcgcc gctaatagag gttgccagta attcagacag gagagatttt gcggtgccag
     6361 gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata cgttcgacag
     6421 cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca aggataaaag
     6481 cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta tcattttgtt
     6541 ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc agatgattat
     6601 tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat attttacgcg
     6661 caataaatgt ttggcgcatt agttgtttga gcgaaaaata tctgggaagc agatcacagt
     6721 aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag gcctaacacc
     6781 acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg accccgttgc
     6841 aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga tcgaacctat
     6901 ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc ggagtgcaac
     6961 atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa ggtgctggtg
     7021 ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa ccgtgctcat
     7081 tgctgtact
//
LOCUS       DAZFKY010000217         7015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFKY010000217 DAZFKY010000000
VERSION     DAZFKY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(169..654)
                     /gene="sixA"
                     /locus_tag="ACHKCL_004211"
     CDS             complement(169..654)
                     /gene="sixA"
                     /locus_tag="ACHKCL_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5765061.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(857..3001)
                     /gene="fadJ"
                     /locus_tag="ACHKCL_004212"
     CDS             complement(857..3001)
                     /gene="fadJ"
                     /locus_tag="ACHKCL_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5765062.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRAQLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3001..4311)
                     /gene="fadI"
                     /locus_tag="ACHKCL_004213"
     CDS             complement(3001..4311)
                     /gene="fadI"
                     /locus_tag="ACHKCL_004213"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5765063.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4491..4775)
                     /gene="yfcZ"
                     /locus_tag="ACHKCL_004214"
     CDS             complement(4491..4775)
                     /gene="yfcZ"
                     /locus_tag="ACHKCL_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5765064.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5147..6487
                     /gene="fadL"
                     /locus_tag="ACHKCL_004215"
     CDS             5147..6487
                     /gene="fadL"
                     /locus_tag="ACHKCL_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5765065.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6852..>7015
                     /locus_tag="ACHKCL_004216"
     CDS             6852..>7015
                     /locus_tag="ACHKCL_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765066.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELI"
BASE COUNT         1785 a         1869 c         1639 g         1722 t
ORIGIN      
        1 acaacttagt ctcatttcgt cagaaattaa tagggaagca gaggaaaaag tgcgatgaat
       61 tctgtatcga agcgtatcaa ctccgtgaag ggttttgagt gaatttctac tattgcaaaa
      121 agacaaatgc ccgatgtaca gcttacatcg ggcatttttt caaacgaatc agatagcttt
      181 cgccatcttc aaattacatg ggctcatctg ccagttgaac tcgccttttc cgctttcatc
      241 aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc ccggacataa
      301 ctcggcgacc agatacccaa ctaacggtaa gtgggagata accaacaccg aagcaacacc
      361 ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc agggcgttaa
      421 ctccggcaga acttctgcac tggaaggcag gttcagacaa tccccaactt cctccagtgt
      481 ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt tttgaccttt
      541 cagccagttc gccatcaggc gagattcgtc acaaccgtta gtggtcagag gacgaaccga
      601 atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca
      661 ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg
      721 tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac ctcaatggcg
      781 taagtatagt caatatgcgt ttacattttg accaacaatc cgccattcag cgcggattca
      841 tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac
      901 gcgcacccat ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac tgcgtggcca
      961 gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag cgaaacggtc
     1021 caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg tcacgcacgc
     1081 tacggataac ctgctcatca acacaacgta cagcttcatt cagcatcaac atcacacacc
     1141 gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa
     1201 tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga tagaaacctc
     1261 gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg
     1321 cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc ccggtgtcga
     1381 ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc actagcgcgg
     1441 catcaatgtg cgctacccgt tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag
     1501 gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc
     1561 cctgtttttt cgccagtttt accgtagtgg cgatggtttg tgctgatgta cccgcatgag
     1621 gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg ccgataactt
     1681 gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac gtattcgaag
     1741 caaagatggt atgagcggcg caattttgct caacttccgc caccatctgt tgtttcaatt
     1801 cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg cgatagtccg
     1861 tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga tgacgacggc
     1921 gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg ccctgcgggt
     1981 tgatatcttt aattctgacc ggaatccccg ctttacaagc agtgacataa gcaataccgc
     2041 cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc ggcgcatcac
     2101 tgccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa gcttgcgatt
     2161 gtggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca ctgctggtgc
     2221 cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga taattgcctt
     2281 gagttttgtg ttctgttttc ttgccgacca ttttgaacag ctgcgcacga cctaacggcc
     2341 ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc ttctttgcca
     2401 gctcaacagc ggcttccagc agaatggagt gcggaacaac atcatccacc agccccagct
     2461 ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat gctgtgctga
     2521 cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta
     2581 cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg tggcacgcca
     2641 gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata accggaatgg
     2701 gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct tccgcttctt
     2761 gcgccgtttt gcaattgccg atcatgttga tgtctgcgcc agcaatgaag ttgtccggtt
     2821 tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc ttaataatgg
     2881 cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc ggtacgtcga
     2941 tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat gtcatttcca
     3001 ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa
     3061 accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc
     3121 ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa atttgctatc
     3181 gtccacttcg ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac gttcactacc
     3241 cagtagctga atattggcca acgtctgggc ggcaaaggct tcgtgcatat caatcaatgt
     3301 cagatctgcc attgtcaaac cggcacgctc cagcgccagc ggtgttgacc aggctggacc
     3361 gagcaacatg tcctgccaga catcgatagc ggtaaatgcg tagctgcgca gatatcccag
     3421 cggtaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg ccgctgcgcc
     3481 atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttacgat caaacgccgg
     3541 acgcagcttt gcgaaatcag caagcgagga attaccgcga atattgttgt cttccgcgag
     3601 cagttgttta taaggaggga taaaggcagt catcacctct tctttgagtt ttccgtctga
     3661 ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt
     3721 gatgccgtag gttttcgcca tttgctctgc agtgtcgccc atccgcaagc cggtagaata
     3781 ttctgctacc gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt
     3841 cagacgctgg ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg ccagtttttt
     3901 actgacgcca attggcaata ccgaagagga atctgcccca ccggcaatcc ccgctcggat
     3961 agtccccgcc atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg tagcgcaagc
     4021 gcggctgacg ctgtaagcat cggtatgcac attcattcca gtaccgagaa caatttcacg
     4081 cgcaatgttg ggagcttcag gcatttgtac gacctgacca aagaccagtt gttcaatcac
     4141 ttcggcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc ctaaatcaac
     4201 cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac
     4261 aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca taacactccc
     4321 ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt tttttacatt
     4381 tagccaagcg gagaaaaggg aaattgggag ctatgacaca gtgaagacag gagaaagaaa
     4441 agaaaacgcc cctacctgtt gctggcagga gcgtacttat ccgagaggaa ttaacgcaga
     4501 cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc
     4561 acaccatcag actcttcagt gaaagtcggg gagattttgc acggttcgga ttccacgcta
     4621 cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg
     4681 cggctgtaag acgctgtgca gtcggagttg tccataatgg tgccaacatc catacagcag
     4741 caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc
     4801 caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt
     4861 taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat
     4921 tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc gtttcttaga
     4981 tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg acctatactc
     5041 tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca
     5101 gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac
     5161 cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc
     5221 ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga
     5281 aggcgcaatt gccgatgatg caggtaacgt cagccgtaac cccgcattga ttaccatgtt
     5341 tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa atatcagcgg
     5401 aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg catgggttcc
     5461 gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt ctattacctc
     5521 taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg tcgggggtac
     5581 aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa ataatgcatg
     5641 gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac gtttcgcagg
     5701 cgatctgggg cagctggttg ctggtcagat tatgcaatct cctgccggga agactcctca
     5761 agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa tcgctcatct
     5821 gaacggcaac cagtggggct ttggatggaa cgccggtatc ctgtatgaac tggataaaaa
     5881 taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag gtaactacag
     5941 cagcgatctt aatcgtgtgt ttaataacta cggtttgcca attcctaccg ccacaggtgg
     6001 cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag tgtcgggtta
     6061 taaccgtgtt gatccgcagt gggcgattca ctatagcctg gcttacacca gctggagtca
     6121 gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga aacatgaagg
     6181 ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg ataactggac
     6241 cttccgtacc ggtatcgcct ttgatgacag cccagttccg gcacagaatc gttctatctc
     6301 cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcgt ttaataaaga
     6361 tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg
     6421 cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc
     6481 gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact ttaactcccc
     6541 cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa cagcctgatg
     6601 gcagctagcc ctttcaaatc agtaagtggt atccacagac aaacctgaag ggaaacgcat
     6661 attttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt tccaggatac
     6721 tcaaccccct ggctattgtg cgctcataca cgcaaattaa agataggttc taaataaatg
     6781 agcgtttttt gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaacat
     6841 ggaataaaat catgctatca tctatttcaa tcaacaatac cagcgcagct tacccagaat
     6901 ccatcaatga aaataacaat gatgaagtta atggattagt acaagagttc aaaagccttt
     6961 ttaatggtaa ggaaggaata agcacctgta ttaaacatct acttgagctt ataaa
//
LOCUS       DAZFKY010000218         6979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFKY010000218 DAZFKY010000000
VERSION     DAZFKY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            163..342
                     /gene="yciG"
                     /locus_tag="ACHKCL_004217"
     CDS             163..342
                     /gene="yciG"
                     /locus_tag="ACHKCL_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5765067.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            428..928
                     /gene="yciF"
                     /locus_tag="ACHKCL_004218"
     CDS             428..928
                     /gene="yciF"
                     /locus_tag="ACHKCL_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5765068.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            974..1480
                     /gene="yciE"
                     /locus_tag="ACHKCL_004219"
     CDS             974..1480
                     /gene="yciE"
                     /locus_tag="ACHKCL_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5765069.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1540..1665)
                     /locus_tag="ACHKCL_004220"
                     /pseudo
     CDS             complement(1540..1665)
                     /locus_tag="ACHKCL_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            1889..1990
                     /locus_tag="ACHKCL_004221"
                     /pseudo
     CDS             1889..1990
                     /locus_tag="ACHKCL_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2400..2635)
                     /locus_tag="ACHKCL_004222"
                     /pseudo
     CDS             complement(2400..2635)
                     /locus_tag="ACHKCL_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2794..3303)
                     /locus_tag="ACHKCL_004223"
     CDS             complement(2794..3303)
                     /locus_tag="ACHKCL_004223"
                     /inference="COORDINATES: protein motif:HMM:NF018156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5765070.1"
                     /translation="MDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLD
                     VFSTYMGQVISEFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQR
                     GVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHF
                     TVRSDCYSV"
     gene            3676..4732
                     /locus_tag="ACHKCL_004224"
                     /pseudo
     CDS             3676..4732
                     /locus_tag="ACHKCL_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4951..5541)
                     /gene="espM1"
                     /locus_tag="ACHKCL_004225"
     CDS             complement(4951..5541)
                     /gene="espM1"
                     /locus_tag="ACHKCL_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO5765071.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            5725..6372
                     /gene="nleG"
                     /locus_tag="ACHKCL_004226"
     CDS             5725..6372
                     /gene="nleG"
                     /locus_tag="ACHKCL_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO5765072.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(6509..6655)
                     /locus_tag="ACHKCL_004227"
     CDS             complement(6509..6655)
                     /locus_tag="ACHKCL_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765073.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT         2161 a         1527 c         1333 g         1958 t
ORIGIN      
        1 caggcaattg ttaatatatc aagaacgttc gtcataatat attttcctac tttcttctcg
       61 ttacgcttaa tttgactaat cctcattagc gactaatttt aatgagtatc gacacacaac
      121 actcatatta atgaaacaat gcaacgcaac gggagaaata acatggccga acatcgtggt
      181 ggttcaggaa atttcgccga agaccgtgag aaggcatccg acgcaggccg taaaggcggt
      241 cagcatagcg gcggtaattt taaaaatgat ccgcaacgcg catctgaagc gggtaaaaaa
      301 ggcggtcaac aaagcggtgg taataaatca ggaaaatcct gattcaccgt taattattaa
      361 tatcagctga taaagataat tgcatctgcg ggccgtatgg ctcgcagtga tttcactgga
      421 gaaaaatatg aatatgaaga ccattgaaga tgtatttatt cacctgcttt cagataccta
      481 cagcgcagaa aaacaattaa cccgggcact ggcaaaactc gcaagagcaa catcaaacga
      541 aaaattaagt caggcttttc atgcgcacct cgaggaaaca catggacaga ttgaacgtat
      601 tgatcaagtt gtggaatcag aatcgaatct gaaaattaag cgcatgaaat gtgtggcaat
      661 ggaaggtctt attgaagaag ctaatgaggt catcgaaagt accgagaaaa acgaagtgcg
      721 tgatgccgca ctgattgccg cagcacagaa agtcgagcat tatgagattg ccagttacgg
      781 gacattagcg acgctggctg aacaattagg ttaccgtaaa gcagcgaagc ttctgaaaga
      841 gaccctggaa gaagaaaagg ccaccgacat caaactgact gatctggccc ttaataacgt
      901 aaataagaaa gccgaaaata aagcctgaaa tatgaacttt aacttttggt cattttataa
      961 agaggacatt ttcatgaatc gtattgaaca ttatcatgac tggttacgtg acgcccatgc
     1021 aatggaaaag caagccgaat ctatgcttga atccatggcc agccgtatag ataattatcc
     1081 tgaactacgt gctcgtattg aacaacatct tagtgaaacc aaaaaccaga ttgttcaact
     1141 ggaaactatt cttgatcgta atgacatttc acgttcagtc attaaagatt ccatgagtaa
     1201 aatggctgcg cttgggcagt caatcggtgg tatattccct tctgatgaaa tagtcaaagg
     1261 ctctattagc ggatatgtct tcgagcaatt tgaaatcgcc tgttacacct ctctattagc
     1321 agcagcaaaa aatgccggtg atacagcctc aattccgatc atcgaagcga ttttaaatga
     1381 ggaaaagcaa atggccgact ggctgattca gcatattccg caaacaactg agaaattttt
     1441 aattcgctct gaaactgatg gcgtagaagc gaagaaataa taccctttat gccatgtcct
     1501 tattgacccc gtatattacg gggtcgtttt tgtgcggaat taaaaacgat atcctgctga
     1561 gaacataaac acccacggat cgaggcgtac gctatcatgt tgctgtgcac cgcccagttt
     1621 ataattggcg gtggtatcga tatccatgta ccacactggt agattttaca aaactcaata
     1681 acatattata tttaaaggaa aaatacagaa tttacgatgc ttttttatac agatctctcg
     1741 tgtttttaac tgattgattt tgttgaactc tttttttgtt ttgcaggagt tactcctctt
     1801 accttattgt ccactcgatg accaacacat tcacaaccca aaccagaata tcctatctat
     1861 gaagcatgta tccgcacgca gattaattat ggtttacatt gttctaccac aatagtcgca
     1921 tcctaaatgg gtgcggttga ggatcaccat ccttgaagag gatgcccgcc actttaactg
     1981 catgacaggg ctatttaaac aatcactaaa aatcgcccaa gagcattctg tttctataat
     2041 gtattccaag agattaaaaa ataatatcat acgttattcg gcagagttcg tatgtactac
     2101 ggcgtcccaa ccatgtccca aaacaaaaat ttctgcccta tacacgttcc aaaaaattca
     2161 acagcaaaga gagtctgctg acgagttgtt gattcactga ttaatacgta aaattgataa
     2221 ctgctggaaa tcatacaata ctcgtacttt cgaaagttcg ctaaccaggt gctacacgcg
     2281 aggtgttgca gttttcttta cgatttatca ttcaatccta atccatgcat gacatgtggt
     2341 gcctgtgggg tgatcctacc tacgtaatgt ggacacagcc ctaatcaagg ttctgttttt
     2401 caaaacagtt ccgtgctgag acagccacaa gtactgtgtc ggcaccaccg attgtaatca
     2461 cactagatct aattaaacac agccgtctgc attatttccc gactcataaa atattagccg
     2521 taaatgcatt cgacttttag tgagtggaag aaactttccg ccctagcatt atcgtaacag
     2581 cgcccctttg cactcatcct gtcatgcagg tatgccgctt cagaagcgcc tgataatgcg
     2641 ttgaaatcag gacggtggct acttgctatt cagttcacga tacatccctt aaggaccagc
     2701 gaatagcaat gagtcacttt attatcaagt ttgatgaccg ctttgatgct aacttttaat
     2761 aagagtctct tcacacaaaa tcgtgtaccg agtctacact gaataacaat cactcctcac
     2821 cgtaaagtga ccgatactgc aatcaaactc acactgctct ctggccacaa gcattgataa
     2881 tgttattggc tctctgctta gtgggtgatg catcccatca ttaattatac gagaaagagc
     2941 catttcatca tataaactac atactctgga accttcagca tttcttaaaa aaacacctcg
     3001 ttgaggaatt gccagcgtaa tcgggcactg taatgcttca tctatgtaag acatttcttc
     3061 aatattaact aaaaatgaac atgaatgtaa tcgccgctgt aacaattcaa ttctgttatc
     3121 attactatgc ataaattcag aaataacctg ccccatatat gtactgaata catcaagaaa
     3181 tgaattgcct ccattcaatt gattttcaac agaatcagcc aatctaatga tatcggctag
     3241 ttctgtatgg cttaaacccg aatatagttc cccttgaaca ggatctacaa taaaagcgtc
     3301 catagcactc tgatagcgaa cagaaaacga tcattctcct attgtgagtc tttccatttg
     3361 atgatctctg gtcgcacgtt gtaaaacttc aacatcttgt gttgacaagt aatttccaga
     3421 agacaaatta aatgaaaatg gcataatttt atccctaaag catatatctt acttttcgat
     3481 aattaacaaa aagccatcct ccacagcaat actccatcag aataattgta ggattatttt
     3541 aatctaattt ataagcataa aatatatact cactaaatag ctgcgcagaa tagtagatca
     3601 tttagaggga actcaacccg attgtgcgat ctgatcaatc gccaaatcaa tacaaatcac
     3661 caaccggact gagcaatgtc gatcatacca ccaatttccc gtgacgaacg acgcctaata
     3721 cagaaagcca tccataaaag gctgacagcc atgctgatgc tgcaccgggg cgaccgtgtc
     3781 agcgacgttg cccgcacact atgctgcgcc cgttcctctg ttgggcgctg gattaactag
     3841 ttcacacagt cgggtgttga gggactgaaa tcattacctg ccgagcgagc ccgccgctgg
     3901 ccgtttgcgc atatctacac actattacgt gaacaggtaa aatattcact cgcgactttg
     3961 gctacaatta ttcacgctgg agtacagaac tgctgacaat aaaaatcaat gagataaccg
     4021 gctgcctgtt acatgctgga accattcgcc gctggttgtc gtctgtgggg cttgtgtggc
     4081 gaagggctgc gccaactcta cgtatccgtg ccccgcctaa agataaaaag atggcaacaa
     4141 tccataacgc actggacgaa tgcagcacag agcatccggt cttttatgaa gatgaagtat
     4201 ttatccatct taatcccaaa atcggtgcgg actggaaact gctcgggaag caaaaacgag
     4261 gggtcacgcc ggaacaaaat gaaaaatatt ctctggacgt agcgctgcac agcgggacag
     4321 gttaagtcag ctgtgtgggc gccaacagca aaagttcggc gctgttcatc agactgctga
     4381 agcggcttaa agctacatac tgtcggacga aaaccatcac gctgctcgag gacaactaca
     4441 ttatccacaa aggccgggaa acccagcgct ggctgaagga taaaccgaag tgcagagtca
     4501 tttatcagcc ggtttaatct ccatgggtga atcattttga atagctatgg cagatacttc
     4561 acgccacaat aacgcgtaat catcagtgcc gctcaatgtg gcaactattg aaaaagttcg
     4621 ccattttatg gaatccgtca gtccattccc cgaaggcaaa catgggctgg caaaaatgtg
     4681 cgatattagg cgcagctatt tagttattcc gtcatttctc ccgcccgtgt aaacactact
     4741 gaatcttgtc gttgtgacaa agctataaat agtcaatcac agcacgtttc tgcgtacgac
     4801 gcgctgcaat tttctttacg atttataact gtacaggtga actatgagtt atttggatac
     4861 atacatatag tgtaacgcct gttgtatatt ttactcatat ctttttacag gatgaaaacc
     4921 aatgatactg gcgcatagcc tcctccaacc ttacccctgt ataacacgac tcatataacc
     4981 tgggatttca tttcgtagtt ttacaatatc aacacccggg gcgatatgtt tctgataaaa
     5041 cttgtctgca agcacgtttg ctatgtaatc tgttgtactg ttttttacag ctcggctcat
     5101 accatcgcaa agagcatcca tttttttgcc aaaagaagca tttcctatta ttatatgatt
     5161 aatagaactt tgtgccgctt taacatcaag agttagttca aactccttac ttacaagaga
     5221 aaagaccatt ttcctctctt cagaagaaat ttttgcattt ttttgaaggc agcaattatc
     5281 aatctcttgg ttgataaccc ttgacggata tactgtccgt tcatcactta cccatccatg
     5341 aatatcttta gtactctgcc tgcttaccgt ctccagtata cagccgctaa atttgttaac
     5401 attaaagcgg atagagcgct ctcccattga acacttcacg acctcatcat tcttcccacg
     5461 gatggtgccc cggaaagaat tatctttatg ataactgata ccaaaagagc tgaaggacac
     5521 acctgtcgca tttactggca taacaaatcc ttttcattaa aatttgctta caaaaattgt
     5581 atacaatcat tttaattaaa aaaatacatg tgtaaatagc attcgttcaa aatttaattt
     5641 tttgtttgaa tcctatcaca tatttagtat cttgactcct gtcaccaata tggttcaata
     5701 acatagacta aagagggaga aaatatgcct gtcatattaa acttttcgag tgaaagagta
     5761 ttgtctgaaa gtgaactgga ggctttacgt catgtcggaa gagttagtca gagtgaacag
     5821 cttgttgtga gaggccgcac tatgagactt catcatattt catttatgga cagttttagc
     5881 gtggaaccgg tttccggcgg actgttggat cgccttagtg caagagggca tcgccttctc
     5941 gcagaaaatc tggagataca gcttaacaga ggccatactt ttttgcaggc attccgttta
     6001 tatatggaac agagccgtgc aaccccatgt acgcgccaaa acgtcagtag tgcaatacaa
     6061 aacaaaatta actctcatgc atttactgtt agtcatcaag acttttcctg tcatgaacaa
     6121 cacctgaatt gcccgataac attatgcatt cctgaaactg gtgtttttgt cagaaatgca
     6181 aaaaactcag aaatatgttc attatatgac cacaatgcac tcactgaact tatccgacgc
     6241 aacgctccac accctctcag ccgtgaaccc tttgttccgg aaatgattgt cagcaaagat
     6301 gaatgccatt ttaatctaat agaacaatat ttttgcatat tagccacaca aaatatatgc
     6361 actagaattt gactgtatgg ttcgatttaa atgttgcaaa attaatttta ctagttattg
     6421 ccatctattt catactccct aattcttgat acaatctgtc tgtcaaaata gtagattaga
     6481 tcgaacttat attacagcta tttagtttct aaaatggaat ttttaacgtt ttatacttca
     6541 cattacaatc tgtaccataa gcctcctcag cgaggttgcc tgaataccct gtcgtgccca
     6601 tttttccatc ggacgcagtt gtctgctctc tcgaactact ggcaataaaa atcaaccaga
     6661 cagcttgatg tccgttacat gcaggactaa tcacgattgg ttaccgttta caaaacttgc
     6721 gtaacattgg aggagaacga ataatattct ctagctggta cgcgttccat agcgaaacgg
     6781 gcaaggtcca acagcaagag ctgaaactca gggggattta tcagtcgatt tattcgccat
     6841 ggatgaatca cgctgtacgg ttatggtagc tcttagcgac aaggcataat catcagtaca
     6901 ccaatgtaac aaccgttgaa aaagactcat tattttatga accagccatc ccatttccag
     6961 agtggtaaat atgatttgt
//
LOCUS       DAZFKY010000219         6935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFKY010000219 DAZFKY010000000
VERSION     DAZFKY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            40..954
                     /gene="shuT"
                     /locus_tag="ACHKCL_004228"
     CDS             40..954
                     /gene="shuT"
                     /locus_tag="ACHKCL_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO5765074.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            974..2311
                     /gene="hutW"
                     /locus_tag="ACHKCL_004229"
     CDS             974..2311
                     /gene="hutW"
                     /locus_tag="ACHKCL_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5765075.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            2324..2818
                     /gene="hutX"
                     /locus_tag="ACHKCL_004230"
     CDS             2324..2818
                     /gene="hutX"
                     /locus_tag="ACHKCL_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5765076.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            2818..3441
                     /gene="chuY"
                     /locus_tag="ACHKCL_004231"
     CDS             2818..3441
                     /gene="chuY"
                     /locus_tag="ACHKCL_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5765077.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3490..4482
                     /gene="shuU"
                     /locus_tag="ACHKCL_004232"
                     /gene_synonym="chuU"
     CDS             3490..4482
                     /gene="shuU"
                     /locus_tag="ACHKCL_004232"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5765078.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4479..5277
                     /locus_tag="ACHKCL_004233"
                     /pseudo
     CDS             4479..5277
                     /locus_tag="ACHKCL_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
     gene            complement(5329..5976)
                     /gene="yhiD"
                     /locus_tag="ACHKCL_004234"
     CDS             complement(5329..5976)
                     /gene="yhiD"
                     /locus_tag="ACHKCL_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5765079.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6040..6366)
                     /gene="hdeB"
                     /locus_tag="ACHKCL_004235"
     CDS             complement(6040..6366)
                     /gene="hdeB"
                     /locus_tag="ACHKCL_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5765080.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6482..6814)
                     /gene="hdeA"
                     /locus_tag="ACHKCL_004236"
     CDS             complement(6482..6814)
                     /gene="hdeA"
                     /locus_tag="ACHKCL_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5765081.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1749 a         1747 c         1681 g         1758 t
ORIGIN      
        1 aatcatgatc attctcaatt tgataagagt tctcatatta tgccaaggat catcaccagg
       61 ccgtttcttt tttccccact gacgttatgt atttcagccg tggcctctgc cgcgaagacc
      121 gcagtaaaac gtaaaaaatt gtttacggca gtactggctc ttagctgggc ttttagcgta
      181 acggctgccg aacgtatcgt ggtcgcagga ggatcgctga cggagctaat ctacgcgatg
      241 ggcgctggcg aacgcgtggt tggtgtcgat gaaacgacat cttatccacc agaaaccgcc
      301 aaactgccgc atattggcta ctggaaacaa ctcagcagcg aaggcatttt gtcacttcgc
      361 ccggatagcg taattacctg gcaggatgcc gggccacaaa ttgtgctcga ccaactacga
      421 gcgcagaaag tcaacgtcgt caccctgccg cgtgtgcctg ccacccttca gcagatgtac
      481 gccaacattc gccaactggc aaaaacgcta caggttcctg aacaaggcga ggcgctggtg
      541 acacaaatca gccaacgcct ggagcgagta cagcaaaacg tggcgaccaa aaaagccccg
      601 gttaaagcca tgtttattct ctccgcaggc ggaagtgcgc cacaagttgc cggtaaaggc
      661 agcgtcgcgg acgccattct ttcgcttgcc ggagcagaaa acgtcgctac ccaccagcag
      721 tacaaaagct acagtgcaga atcactgatt gcggctaatc cagaagtgat tgtcgtaacc
      781 tcacaaatgg tcgatggcga tattaatcgg ctacgttcta ttgctggaat tacccacact
      841 gccgcatgga aaaaccagcg cattatcacc gttgatcaaa acctgattct gggaatgggt
      901 ccgcgtattg ctgatgtcgt tgagtcttta caccagcagc tttggccgca ataaatggat
      961 ttaaatcaga cgaatgaacg caaataacac cctggatctc acgccacatt ttgctttaga
     1021 cggtgatcag ccatttaaag acaggcgcgc catgatgccg tttcgtggag ccattccggt
     1081 tgccaaagag caactggcac agacctggca agagatgatc aatcaaacgg catcacctcg
     1141 taagcgactg gtttacctac acattccttt ttgtgcgaca cactgcacgt tttgtggttt
     1201 ttatcagaac cgttttaatg aagatgcgtg tgctcattat accgacgccc tgattcgtga
     1261 aatagaaatg gaagcggaca gcgtattgca tcagtctgcc cccatccacg cggtctattt
     1321 cggtggcggt acgccttccg cactttcggc acacgatttg gccagaatca tcaccacatt
     1381 acgagaaaag ctgccactgg cccctgattg tgaaatcact atcgaaggac gggtattgaa
     1441 ttttgacgct gagcgaatcg atgcttgcct tgatgcagga gcaaaccgct tctcaattgg
     1501 tatccagtcg tttaacagca aaatccgcaa gaaaatggcc cgcacctcgg atggcccaac
     1561 cgctattgcg tttatggaaa gcctggtcaa acgtgaccgt gctgcggtgg tctgtgacct
     1621 gctgtttggc ctgccgggtc aggatgcgca aacctgggga gaagatctgg ctattgcccg
     1681 cgatatcggt ctcgacggcg tcgatctcta tgcgctcaat gtcctgtcca atacaccgct
     1741 gggcaaggcc gtggaaaatg ggcgtactac cgtaccctca ccggcagaac gtcgcgatct
     1801 ttatctgcaa ggatgtgatt ttatggacga tgccggttgg cgctgcatca gtaacagcca
     1861 ctggggccgt accacacgcg aacgcaatct ctataacctg ctgattaaac aaggtgccga
     1921 ttgtctggcc tttggttccg gagccggcgg ttcgattaat ggttactcct ggatgaacga
     1981 acgcaatctt cagacctggc atgaatccgt cgcggcaggc aaaaaaccgc tgatgttgat
     2041 catgcgtaac gccgaacgta atgcgcaatg gcgtcatacc ttgcagtcag gtgttgaaac
     2101 agcgcgcgta ccgctggatg aacttacgcc acatgccgaa aaactcgcgc cgttactggc
     2161 tcaatggcac caaaaaggct taagtcgcga tgcttcaact tgcctgcggc tgactaatga
     2221 aggtcgtttc tgggcaagca atattttgca gtctcttaat gaactaattc aggtacttaa
     2281 tgcgccagcg attatgcgtg aaaaaccata acaggagtga tttatgagcc atgtctcgtt
     2341 acaagaattt ttgaaaacgg agccggacgg tacgctggag gtggtagccg aacaatacaa
     2401 caccacactg ctggaggtag tgagaaatct accctcctct accgtagtgc caggcgataa
     2461 attcgatact gtctgggata ccgtctgcga atggggcaac gtcaccacgt tagtacatac
     2521 tgccgatgta atcctcgaat ttagcggcga actgccttcg ggtttccatc gccacggcta
     2581 tttcaaccta cgcggaaagc acggcatgtc cgggcatatc aaagcagaaa actgcacgca
     2641 tattgcctta atcgaacgta aatttatggg catggacacc gcgtccattc tctttttcaa
     2701 caaagaaggc agcgccatgc tgaaaatctt cctcggcagg gatgaccatc gtcagctcct
     2761 gagcgaacaa gtcagcgcct tccatactct ggcggcatcg ttaaaggaac acgcctgatg
     2821 acgccgtggc tactctttgg cgcaggcgga aaaggtgtgg gtgccagaac gcttgaactg
     2881 gcgctggcgg aacaacgtcc ggtcgttgct gtcattcgtc atgccgatgc cgccacgaaa
     2941 ctggcgcaac aaggcgtaca ggtttttaca ggcgacgcct gtgatgccag cgtagtcgcg
     3001 gctgcttgcc gcgcagctgg cccggatgcg cttatcatct caacaatggg aggagctcag
     3061 gattatctgg cgcacagaac agtgattgat gaggccgaaa aggccggcat cagtcgcatg
     3121 attctggtga cctctttggg atgcggcgat agctggccgt ttttatcgga gcgggcaaag
     3181 gctgcgtttg gtcaggcggt acgggaaaaa acgctggcgg aaagctggct gcaaaccagc
     3241 cagctagact acgccattct ccgccctggt ggtctgcttg acggcgcagc aacaggcaaa
     3301 gcacaacgga tacaaaatca ggagtgtcat ggttttgtcc gccgtgccga tgtcgccgca
     3361 catatccatg aactggcaaa tgcgccagca cttaaccagc aggtctacag tctgattgaa
     3421 ccggacctga aaccggcgtg ataccccggc gcggcatgtc cgcgccgtaa tgtcgtaacg
     3481 gagttttgcg tgctcaagga ttcattttca tccgccaggg tctttatggg actatcacta
     3541 ttattgcttg ccctggtgct gttcggtgct agtcagggag cgttaaagat cagttttgat
     3601 gccctttttg atgaggaata ccgcgatatc tggctcaata ttcgtctacc acgggttttg
     3661 ctggcggtgc tggtaggtgc agcgttggca accgcagggg taattatgca ggggcttttt
     3721 cgcaacccaa tggctgaccc aggattactc ggcgtcagta gcggttctgc attaatggtt
     3781 ggcgttgcaa ttgtgctgcc cttctccttc ccagtggtgc tggtgctcta tgagcaaatg
     3841 gtgtttgcca ttgccggaag tttagtagtc tgcgccatca tttttctcat cacgcagcgc
     3901 catcgcgatg gcagcatgat gcaattatta ctcgccggta tcgccatcaa tgccctgtgc
     3961 ggcgcagcga tcggcatcct gagctatatt ggcgatgagc agcagctacg acaactcaca
     4021 ttgtggatga tgggcaatct tggacaggcg caatggccga cgttattggt tgccagttca
     4081 ttcatccttc cggccattat cgcaacaact tgtctcgccg gaacgctgaa tttactgcag
     4141 ctcggtgatg aagaagccca ttacctcggc gtgaacgtta agcgtaaacg ccagcaatta
     4201 ctattagtga gctcactgct cgttggtgct gccgtatcgg taagcggcat tatcggcttt
     4261 attggcctgg tgatcccgca tctgattcgc atgactaccg gagcaaatca ccgctggctc
     4321 atcccttgtt ccgccctcgc cggagcctgt ttattgctga tggcagacac gcttgcccgc
     4381 acgctggtac agccagcaga aatgcccgtg ggattattaa ccagcctgct tggtggccct
     4441 tattttatgt ggttgattct gcgaaatcgg aggatcacat gatcagcgcc cagaatctgg
     4501 tttatagcct tcaggggcgg cgtctgactg ataatgtctc gctgactttt cccggtgggg
     4561 aaattgttgc cattctcggc cctaatggtg cgggaaaatc tacgctgctt cgccagttaa
     4621 ccggctatct tcaacctgat agcggcgaat gtcggttgtt taataagcca ttaaatgaat
     4681 ggtcgattac tgaactggcg aaacaccggg ccgtgatgcg tcaaaatagc cacatggcgt
     4741 ttccattcag cgtacaagaa gttatccaga tgggtcggca tccgcatcgt actggtaatc
     4801 aggataatga aacagcacaa ataatggcgt tatgtgactg tcaggcgtta gcaaaccgcg
     4861 actatcggca attatccggt ggtgaacaac agcgcgtgca attagcgcgc ctgctggtgc
     4921 aactctggga accaactccc tcaccgaaat ggttatttct tgatgaacca acttcggcgc
     4981 tggatattca ccatcagcag cacttgtttc gtttattacg ccagttagtt cacgagcggc
     5041 agttcaatgt ctgctgcata ttgcacgatc taaatcttgc cgcgcattat gcacgatcta
     5101 aatcttgccg cgcattatgc agaccgtgtt gtattaatgc aaaaaggaaa ggttatcgca
     5161 aatggtaaac cacaggacgt attgacacag caggcgctga caatgctcta cggcgcggat
     5221 attaccgtac ttaaagatcc tgccaaccat tcaccactga tcgtcctcga tcattaaaag
     5281 caacaggagt accgcactgg tactcctgcg aataaataac tatacagatt agctaattga
     5341 aacgcctttc acccctgcca taccttttaa taatcgcaac agatcttcta tcgacgttgt
     5401 ggcatgaagc tggatatcaa tggcgaccac ttcatgatct tcattttcct gcaacgaaac
     5461 taaatcagtt tttatttttt gctgcttaaa ccagtcgagc attgataccg tattaccgtt
     5521 cactaatgtc agctgtaaat ggtaattttt gttcatcagg cggaaggtta attgatggaa
     5581 gacttccagc accagcaagg tcatcactga accataaata cccagttcgt acataccact
     5641 gccaataacc atacctatgg cggcggtcac ccagatatcc gctgccgtcg tcaaacctac
     5701 aatattttgg ttacgaacga gaatgttacc tgcaccgata aacccgacgc ccgtcaccac
     5761 ctgagcagca atacggctgg ggtccagtcc gacgtgatct aaagacagca cgtcagcaaa
     5821 accatatttc gaaacaatca taaacagtgc gcttcccatg ccaattaata catgagtgcg
     5881 taaccctgct cctttgccgc gcatttgcct ttccatgcca atagcgccac aggcaattgc
     5941 cgcgagtata agacgtataa taaattctgc tgtcatataa aaatgcaaaa aggagcagca
     6001 agatggctca acttgccact cctttttact tgtacctcat taattcggca agtcattaga
     6061 tgcttgattt ttgaaggtat acaaattttt ctgcgggttt ttcttacagt attcgatcac
     6121 tttaggaatt tgagtgagat cggtttcatt taaagtaact gtatcgccac ctttatatac
     6181 tgtttcttca tgcagcatcc accatgcaac cggggtcatt gcttttggat tcagatcaat
     6241 aaattcctgg caggtcatat ctttagcgga ttcattggct gccagcgcag attgtgcgtt
     6301 caccagtgac aaagccgcta cagcgcccat aaaaataaac gctttacgga gagatgaaat
     6361 atttattttg taacccattc aatatagaga ttatatatga acaggaggaa tagctgcttt
     6421 taaagaagat attccggcct gttcaatatg caaggaagta cgatgtaacg cattcccgga
     6481 attacatatc tttcttaatt ttgtcccatt cgcctttaac tttatcttta aagttggctt
     6541 gtttatcctg agtacaagcc tgaacgatag ctggggttac ggttgcaata ccctgaacat
     6601 ctaaaaccgc atcttctggt ttatctttgt tgttcagcgc ttcagcaaaa ccaactgcag
     6661 ttggctggaa ggattcgtcc acagccagga aatcttcaca ggtccaggag ttgaccggtt
     6721 ttttgttatc agctgctttt tgcgcatccg ctgcattgct cacaactggc agaagaagca
     6781 gaccaccaag aataacgcct aatacttttt tcatcgtaat atcctcaact ataaagtgaa
     6841 agagccgtca cgaatcaatt tcgacactga ggttataacc tggttttctg tatatgtcat
     6901 gttgatggaa aatatcaaaa tcagatattt ttatt
//
LOCUS       DAZFKY010000220         6873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFKY010000220 DAZFKY010000000
VERSION     DAZFKY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACHKCL_004237"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACHKCL_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5765082.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACHKCL_004238"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACHKCL_004238"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5765083.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="ACHKCL_004239"
                     /pseudo
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="ACHKCL_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKCL_004240"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKCL_004240"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5765084.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKCL_004241"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKCL_004241"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5765085.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAVALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKCL_004242"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKCL_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5765086.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1722 a         1609 c         1800 g         1742 t
ORIGIN      
        1 taatgagcac agtgataatt aatgacgaaa tgaatgacgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtg
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc aacaatttgt ctgacaaatt ggcttttcct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag ttaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctgc acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggtttcc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 tagcctggcc gccagtggcg caaaaggttt cgttatttgt actccggacc cgaaactcgg
      721 ctctgccatc gtggcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttcct ctggtgatga tggcggcgac
      841 caaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac ctccaacgaa ctggatacgg ctcgtcgccg
      961 tactacgggt tctatggatg cgctgaaagc tgcaggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatccccgg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gtatcggtat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaggcacag gcaaccggct tctacggttc
     1261 cctgttgcca agcccggacg tacatggtta taagtccagc gaaatgcttt acaactgggt
     1321 agcaaaaggt gttgaaccga ccaaattcac cgaagttacc gacgtggtgc tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctt tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggtgtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa
     1981 cgctaaaatt atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaaccgcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg
     2401 catgtttggc gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcctg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 taaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatta tgaatctctc
     2701 aggtggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcacgaaa tatataacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgcgaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg ttctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgcttt ttatcgcctg tgccattttt gtcccgaact ttgccacctt tattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgagc gggtgtcacc
     3301 acggcggtgg ttatcaatct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg
     3361 cttggcattc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacga cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttccgac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc caactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgctgctg
     3601 aataaaacta cctttggtcg taataccctg gcgattggcg ggaacgaaga ggctgcgcgt
     3661 ctggcgggtg taccggttgc tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tctgcgatag ccggaattat tctggcttca cgtatgacca gtgggcagcc aatgacgtcg
     3781 attggttatg agcttattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcgttgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaac acaataagaa aagagaagga ggagaaccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat ctcgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt acctgacaat attctgcaag gacttcagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 ggacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacgc atatcgttca tctgccggat gcgattgcac gtgatattaa
     4501 cgtgcaactg catacggtca tcgcccagta tcccggtgcg gagctggaga cgaaagggat
     4561 ggcttttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg agaggtacca gtaaaggtga ggcgattgct gcctttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 agtcgttaac cgactgggcg gaatgtcagt aaaaattggc acaggcgcaa ctcaggcatc
     4861 atggcgactg gcgggcgtac cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagat gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaagg gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcctgctt
     5221 ttcattatcg cctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgccttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacagcctgg ggcaaagcgt ttcgaacaga agtttacccg attggcattg
     5641 agccaaaaga aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccaga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagccg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa
     6061 aagagtatgt tgccgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa tccctacgat cgtgacgaag
     6181 ttgcagttgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttcctaa gcttgtgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggcaatcac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatatttgc gacaaacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttcc tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcagcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacag cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg aga
//
LOCUS       DAZFKY010000221         6794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFKY010000221 DAZFKY010000000
VERSION     DAZFKY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..394
                     /locus_tag="ACHKCL_004243"
     CDS             98..394
                     /locus_tag="ACHKCL_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765087.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            702..818
                     /locus_tag="ACHKCL_004244"
     CDS             702..818
                     /locus_tag="ACHKCL_004244"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765088.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1037..2422
                     /locus_tag="ACHKCL_004245"
     CDS             1037..2422
                     /locus_tag="ACHKCL_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5765089.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
     gene            3243..3806
                     /locus_tag="ACHKCL_004246"
     CDS             3243..3806
                     /locus_tag="ACHKCL_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5765090.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            3961..6321
                     /locus_tag="ACHKCL_004247"
     CDS             3961..6321
                     /locus_tag="ACHKCL_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5765091.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(6483..>6794)
                     /locus_tag="ACHKCL_004248"
     CDS             complement(6483..>6794)
                     /locus_tag="ACHKCL_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765092.1"
                     /translation="DYHYKKAAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDAAYDD
                     RMTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMANYQLDEQLLEGCR
                     P"
BASE COUNT         1886 a         1316 c         1657 g         1935 t
ORIGIN      
        1 atttattgtc ttttttctca acccattttt ttacgcagac aaatttaccc ggggaataca
       61 aactgtaacg acagttcacc acactatggg ggataaaatg ccagatcaat ccggacactg
      121 caggactcct tcacgcatct tacggatgcc tgaactgagc caattgttag gtatatctcg
      181 ttcgacaatt tacgaaaaaa tgaatccgtt atcgaaatac tacgatgcaa ccttccccag
      241 gccggtacgt cttggtagtg gttctgtggg ctggcgttca tcagctattg acgaatggct
      301 tactttacat actgtacctg ccagatcagc agtcaaaggt gttaatgacg aagtgagaca
      361 tacgctttct tcagatatga ccatgagcag ataagttttt gtcatcacgt gatcacctca
      421 ggaggaaaaa gaagcataag aacaatttag ccagaagttt aattgataaa cagaatttcc
      481 agcattcatt gttgaaagag cagctattgt cgctattgta gcggtaattg ctgctataac
      541 cgaaacgtat tacgaaaaag gaagcattta tgccttacat caaaccggat atgtcgtatt
      601 cagtatatgc atgaccatca atgataggga aggcaataat taaagctcaa tacaataaac
      661 aagtacctct gactctggtt gcaatgtcag tattaacagc gatgtcaatt gcctgtcaga
      721 atcaggttga cgtatgcagt cccggaaatt tacgtggggc cgttaatatt tacactatgg
      781 ttctggagaa agggaaaaga ccgggggaaa ggtattagtg aaataatttc cggaggtatt
      841 aagaatgaga aatatgaatg atatttcgtg gtgaataata cagtcttcac tgaattaacc
      901 ttatcggttt attaaacatc agatctacaa atgctctgta tttctcagag catcaggtct
      961 gaatagcagc tattttcgct attgtagcgg gatttgctgc tattttacta acccattaat
     1021 gaaaagagag gcttttatgt gcttacttgc accagaaaat ccttatccga tatatgcact
     1081 accaccgctg gtgagaaatg caataattga aactcaaaaa aatacacagg cacctttggc
     1141 tatggtggca acatccgcat taacagcgat ctcaattgcc tgtcagaatc agattgacgt
     1201 gtgcagacct ggaaatttac atgggcctgt taatctttac tctctgattc tggctgattc
     1261 cggtgaaagg aaaacgaccg tggataaagt gtttatgaaa gcattttatc tcagggatga
     1321 agccctggcg gatgaatacg cgaaactggt tgagaattac agtacagaaa aggaaatatg
     1381 ggagcaaaaa caaaaagcgc tggaatcaaa atttcataaa gagattcgtg ccggtaaaga
     1441 ttacaaggca acagaatcag agcttgaaac gcatctgaat aaacctcctg ttccgccgca
     1501 gatacgtcga acaattttta atgagacaac gatagaggga atgttaaaat attactccga
     1561 tagcaatcgc tcttttgctc ttgtatccag tgaagggggg gtaatttttg acagccgggc
     1621 catgagtaaa ctgggaatta ttaacacttt gtgggatgga ggttctcttt tcatcgacag
     1681 gaaatcatct cccggaatta atttgaagga gccaagactg acgatatcgg tgatgattca
     1741 gcctgatgtt taccacaaag gtttttgtac gcgaaaaaaa gaaattgtga aaacgtcagg
     1801 acatcatgca aggtttttga tgtgtcaacc aacatcaacg caggggacaa ggataatgat
     1861 caatgagctt attgatgaaa gtctggcgat gagtggtgaa cgacgttgcc ttcacttttc
     1921 tcctcaggct gccagaatct ggacggatta ttacaatgat gtggaatcga agctgggggg
     1981 attggggcct ttaagacatt gccgggaata tgctgctaaa aatgcagagt atatggcaag
     2041 actggcagga cttatttacc attcgagcgg tgaagagggg gagatttccc cttacactgc
     2101 agaaatggcg agagaattag caatatggta cggtaatgag tatgtgcggt tgtctaatcc
     2161 gttaactttt gacaactctg ccctgaccgt acctgtgcga ctcattccag aggagcttga
     2221 acttttcaac tggataaaaa gctattgtat tgagaagggg atcctctgta tgaaaaaaaa
     2281 tgacatttta cagcgtggtc cgaaccgttt ccggaaaaag gataaaatca actggttact
     2341 ggatttactg tatgaacaaa acagagttgt accggttatt gagggaaaaa cgttgtgtgt
     2401 cgcacctaac tttgacctct gaacgactta atcgttgagt acatatagtt tcctctgcta
     2461 cttgcggagg agactaatgt ctttaccatg tcagcgttgt attagaaaac tctggtacag
     2521 gtcagtcagt aaatataact ggtgtaacca ctatttacta ttggatagag tgttgctcag
     2581 gatattgtcg gctttagtct gaggtgaggt ggggccttca ttgttttcgt gttcaataaa
     2641 taatttaggt taattagtat tattagtgga agttatgtga tctgttatgt tgtcttgtac
     2701 gcacttaaca ctggttacgt accggaacac tattagagta tgttatggct ctgttacagt
     2761 ttataggtat gaaaaaatat aatctttcct gtatgaaata atattcttat ggtgtgtggg
     2821 gtatggtctg tataccatca tttttatggt gaagttaaat atctgtagag gccttaagct
     2881 gggtcgctgt ttttacttaa tggtacagaa gtattaatag cggtgttgcc tgtggctacc
     2941 cgatactggc ttttgtgaat gtcagattac ttcctgtaag tgattgaatg gtacttcctt
     3001 aaactcaatt ctatgaggtg tgtttttacc attgtaattc gtactggtcc tcagtttaag
     3061 tgagtcttac tgtactctga aatattacct ctttggagta atgaggtaat acgaaacagt
     3121 tttatcaggt tgtctgtgct gctggttacc tcgtttatta atgccgcggt acggcgtttc
     3181 tgtactctca gtatttatgt aattccacct tcctgtaact tttataaagt aaggactata
     3241 aaatgtataa tgctaatccc aattacgaaa tggacttcat gattctgaaa gacgtgaatg
     3301 aacacatgga aggtctgttt caacgttttt ctaaactgtt acctttcaga attgattttg
     3361 cctacaggaa agacacgccc agttttggtc attcctgtag gcacagtatg tgtattgaaa
     3421 tgcaccgctt acttagtgaa acccaaacga tgctggcggg atattactgg gtaatggaat
     3481 atacgtcaaa caagggactt catattcatt ttatcggcta cctggatggg caacgtcaca
     3541 acaaatctta tcggatatcc aggcaactgg gagatatctg gaggcggatc acggaagggg
     3601 agggttattt tcacctgtgc agggctaaag acaaataccc ggtgcgtatc gatcacgtaa
     3661 ttcactatag tgataaatct gcggtagacg acttacgtta cgcccttagc tatctggcca
     3721 aacaggatca gaaagaacac ggtattattc tgaggcgtag caggcttccc gaaaaaagta
     3781 accggggccg accacgacat aactgaatta cagctgtatt aggtattaga attaagtttc
     3841 tgtaagttgt tgggaggtgc ttgagttaat aggcagaaga gggcggggcg ctttgtcatc
     3901 agtgtagggg acccgaattt ctgccgtggt gcctcagtgg atcggataca ggtaacgaat
     3961 atggttcata aatctgacag tgatgaatta gctgcattga gggcagaaaa tgcccgtctg
     4021 gtctcattac ttgaagctca tgggattgaa tggcgacgta aaccgcagac tcctgtgcag
     4081 cgcgtttctg tattatccac cgacgagaag gttgcattat ttcgtcggtt gtttcgtggg
     4141 cgtgatgatg tatgggcact tagatgggaa agtaaaacca gcggcaaatc agggtactct
     4201 cctgcctgcg ctaacgaatg gcaggcgaga atatgcggta aaccccggat aaaatgtggg
     4261 gactgcgctc accgtcagtt gattcctgta tccgatctcg tcatctacca ccatctggcc
     4321 ggtacccata ctgtcgggat gtatccgttg ctggaagatg attcctgtta ttttctggca
     4381 gttgattttg atgaagctga atggcagaag gatgcatccg cattcatgcg atcctgtgat
     4441 gagctgggtg tacctgctgc gctggaaata tcccgttcac gtcagggggc gcatgtctgg
     4501 atattttttg cctcacgagt ttcggcccgc gaagctcgcc gtctggggac tgctattatt
     4561 agctatacgt gtagtcggac ccgacagctg cgattggggt cttatgaccg attatttcct
     4621 aatcaggata ctatgccaaa agggggattt ggtaatctca tagcgttacc tctgcaaaaa
     4681 agaccgcgtg aattgggggg aagcgttttt gttgatatga atctccagcc ttatcctgat
     4741 cagtgggctt ttcttgtatc ggtgaccccg atgaatgtgc aggatattga accgacgata
     4801 ttacgggcta cagggagtat ccatcctctg gatgtgaatt ttatcaacga agaagacctg
     4861 ggtacgccgt gggaagagaa aaaatcatca ggaaacagac tgaatatttc tattgcagaa
     4921 ccgctgaaaa tcacgctggc aaaccagatc tatttcgaaa aagcgcaatt acctcaggtg
     4981 ctgattaacc gacttattcg gctggcagca tttccgaacc ctgagtttta taaggctcag
     5041 gcaatgcgta tgtcagtctg gaataagccc cgtgttatag gttgtgcgga gaattacccg
     5101 caacacattg cgttgccccg gggatgtctg gacagcgtat tatctttcct tagggacaac
     5161 aatattgctg cagaattaat cgataaacga tttgccggga cggaatgtaa tgccgttttt
     5221 atgggaaacc tcagagcgga gcaggaagag gccgtttcgg cattactccg ttatgacact
     5281 ggtgtgcttt gtgcgccaac ggcttttggt aagacagtta ccgcagcggc agtgattgcc
     5341 aagcggaaag tgaatacact gatactggta caccggactg aattgctgaa gcagtggcag
     5401 gagcgtctcg cggtgtttct gcaggccggt gacagtattg gtattatcgg gggaggtaaa
     5461 cataaaccct gtggcaatat tgatattgcg gtggtgcagt ccatatccag acaaggagaa
     5521 gttgaacctc tggtcaggaa ttatgggcaa atcattgtgg atgagtgcca tcatattggc
     5581 gcggtttcat tttctgcgat tctgaaggaa acgaatgcca gatatctgct tggcctgacg
     5641 gcaacaccaa tccgacggga tggtctgcat cccattattt ttatgtactg tggtgccatt
     5701 cgccatacag cggtccgccc gaaggaaagc ccacataatc tggaggtact gatccgttcc
     5761 cgttttacat ctggtcattt accatcggat gcgagaatcc aggatatttt cagagaaatt
     5821 gctctggatc atgacagaac ggtggcgata gctgaagaag ccatgaaagc tttcgggcag
     5881 gggcgaaaag ttctggtact gactgaacgt acagatcatc tggatgagat agcatcagtg
     5941 atgaattcac tgaaattgtc tccctttatt ctccatggtc gactatcgaa aaaaaagcgt
     6001 gcgatgctga tatccgggct gaatgctctt cctcccgatt ctcctcgaat tttgttgtca
     6061 acaggcagac ttattggtga gggatttgac caccctccgc tggatacgct gattcttgcc
     6121 atgcctgtgt catggaaagg gacattacag cagtatgcag ggcgtcttca cagagagcat
     6181 accggtaaaa gcgatgtcag gatcattgat tttgtggata ccgcgtatcc tgtgctgctc
     6241 agaatgtggg ataaacgtca gcggggttat aaagcgatgg ggtacaggat tatagctgac
     6301 ggcgatgaat cagtgattta attttaagga gtactttacc ctttttaaat aatttgagct
     6361 ccccgggttg ttcgggcaac aaagagtccc agtatacagg cttcaggaat atgttgtagt
     6421 atcaggcttt tcatcgcttt aaaatgacta ccggcagtga gaacgtcatc aaaaataaca
     6481 atctagggcc tgcacccttc cagtaattgt tcatccagtt ggtagtttgc cataatcgct
     6541 tctggtaagg gccggtttgt tgcggtatga gaggctattg ttgatacgtt ttgcacaacc
     6601 agttcccaga aatccagcca tggttcgtag gtttgcgcaa ttcgcagaat ttctgtcata
     6661 cggtcatcgt aagcggcatc tgtacggcat ttggatggtg gtacgggaac aaatgtgtat
     6721 tcctcaagat taccgatatt tgatatgagc atctgagcaa catactcaat agcagccttt
     6781 ttatagtggt aatc
//
LOCUS       DAZFKY010000222         6794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFKY010000222 DAZFKY010000000
VERSION     DAZFKY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            656..1915
                     /gene="codB"
                     /locus_tag="ACHKCL_004249"
     CDS             656..1915
                     /gene="codB"
                     /locus_tag="ACHKCL_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5765093.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1905..3188
                     /gene="codA"
                     /locus_tag="ACHKCL_004250"
     CDS             1905..3188
                     /gene="codA"
                     /locus_tag="ACHKCL_004250"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5765094.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3321..4220)
                     /gene="cynR"
                     /locus_tag="ACHKCL_004251"
     CDS             complement(3321..4220)
                     /gene="cynR"
                     /locus_tag="ACHKCL_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO5765095.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4330..4989
                     /gene="cynT"
                     /locus_tag="ACHKCL_004252"
     CDS             4330..4989
                     /gene="cynT"
                     /locus_tag="ACHKCL_004252"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO5765096.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            5020..5490
                     /gene="cynS"
                     /locus_tag="ACHKCL_004253"
     CDS             5020..5490
                     /gene="cynS"
                     /locus_tag="ACHKCL_004253"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO5765097.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5496..6677
                     /gene="cynX"
                     /locus_tag="ACHKCL_004254"
     CDS             5496..6677
                     /gene="cynX"
                     /locus_tag="ACHKCL_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO5765098.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
BASE COUNT         1403 a         1814 c         1899 g         1678 t
ORIGIN      
        1 ctgatgcgac gctgatgcgt cttatcatgc ctacaggcct gaaccgtagg tcggataagg
       61 cgctcgcgcc gcattcgaca ccatgcgccg atgcctgatg cgacgcaggc gcgtcttatc
      121 atgcctacag gcctgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaccatgc
      181 gcagatgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga accgtaggtc
      241 ggataaggcg ctcgcaccgc atccgacacc gtgctcagtg cctgatgcga cgctgatgcg
      301 tcttatcatg cctacaggcc tgaaccgtag gtcggataag gcgctcgcgc cgcatccgac
      361 accgtgcgca gatgcctgat acgacgcagg cgcgtcttat catgcctaca ggcctgaacc
      421 gtaggtcgga taaggcgctc gcgccgcatc cgacaccgtg cgtagttgcc tgatgcgacg
      481 ctgacgcgtc ttatcatgcc tacccactgt ttttacaccg ataattttcc ccccaccttt
      541 ttgcactcat tcatataaaa aatatatttc cccacgaaaa cgattgcttt ttatcttcag
      601 atgaatagaa tgcggcggat tttttgggtt tcaaacagca aaaaggggga atttcgtgtc
      661 gcaagataac aactttagcc aggggccggt cccgcagtcg gcgcggaaag gggtattggc
      721 attgacgttc gtcatgctgg gattaacctt cttttccgcc agtatgtgga ccggcggcac
      781 tctcggaacc ggtcttagct atcatgattt cttcctcgca gttctcatcg gtaatcttct
      841 cctcggtatt tacacttcat ttctcggtta cattggcgca aaaaccggcc tgaccactca
      901 tcttcttgct cgcttctcgt ttggtgttaa aggctcatgg ctgccttcac tgctgctggg
      961 cggcacccag gttggctggt ttggcgtcgg tgtggcgatg tttgctattc cggtgggcaa
     1021 ggcaaccggg ctggatatta atttgctgat tgccgtttcc ggtttactga tgaccgtcac
     1081 cgtcttcttt ggcatttcgg cgctgacggt tctttcgttg attgcggttc cggctatcgc
     1141 ctgtcttggc ggttattccg tgtggctggc cgttaacggc atgggcggcc tggacgcctt
     1201 aaaagcggtc gttcccgcac aaccgttaga tttcaatgtc gcgctggcgc tggttgtggg
     1261 gtcatttatc agtgcgggca cgctcaccgc tgactttgtc cgctttggtc gcaatgccaa
     1321 actggcggtg ctggtggcga tggtggcctt tttcctcggc aactcgttga tgtttatttt
     1381 cggtgcagcg ggcgctgcgg cactggggat ggcggatatc tctgatgtga tgattgctca
     1441 gggcctgctg ttgcctgcga ttgtggtgct ggggctaaat atctggacca ccaacgataa
     1501 cgcgctctat gcgtcgggtt taggtttcgc caacattacc gggatgtcga gcaaaaccct
     1561 ttcggtaatc aacggtatta tcggtacggt ctgtgcatta tggctgtata acaattttgt
     1621 cggctggttg accttccttt cggcagctat tcctccagtg ggtggcgtga tcattgccga
     1681 ctatctgatg aaccgtcgcc gctatgagca ctttgcgacc acgcgtatga tgagtgtcaa
     1741 ttgggtggcg attctggcgg tcgccctggg gattgctgca ggccactggt taccgggaat
     1801 tgttccggtc aacgcggtat taggtggcgc gctgagctat ctgatcctta acccgatttt
     1861 gaatcgtaaa acgacagcag caatgacgca tgtggaggct aacagtgtcg aataacgctt
     1921 tacaaacaat tattaacgcc cggttgccag gcaaagaggg gctgtggcag attcatctgc
     1981 aggacggaaa aatcagcgcc attgatgcgc aatccggcgt gatgcccata actgaaaaca
     2041 gcctggatgc cgaacaaggt ttagttatac cgccgtttgt ggagccacat attcacctgg
     2101 acaccacgca aaccgccgga caaccgaact ggaatcagtc cggcacgctg tttgaaggca
     2161 ttgaacgctg ggccgagcgc aaagcgttat taacccatga cgatgtgaaa caacgcgcat
     2221 ggcaaacgct gaaatggcag attgccaacg gcattcagca tgtgcgtacc catgtcgatg
     2281 tttcggatgc aacgctaact gcgctgaaag caatgctgga agtgaagctg gaagtcgcgc
     2341 cgtggattga tctgcaaatc gtcgccttcc ctcaggaagg gattttgtcg tatcccaacg
     2401 gtgaagcgtt gctggaagag gcgttacgct taggggcaga tgtagtgggg gcgattccgc
     2461 attttgaatt tacccgtgaa tacggcgtgg agtcgctgca taaaaccttc gccctggcgc
     2521 aaaaatacga ccgtctcatc gacgttcact gtgatgagat cgatgacgag cagtcgcgct
     2581 ttgtcgaaac cgttgctgcc ctggcgcacc gtgaaggcat gggcgcgcga gtcaccgcca
     2641 gccacaccac ggcaatgcac tcttataacg gggcgtatac ctcacgtctg ttccgcttgc
     2701 tgaaaatgtc cggtattaac tttgtcgcca acccgctggt caatattcat ctgcaaggac
     2761 gattcgatac gtatccaaaa cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg
     2821 gcattaacgt ctgctttggt cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg
     2881 cgaatatgct gcaagtgctg catatggggc tgcatgtttg ccagctgatg ggctatgggc
     2941 agattaacga tggcctgaat ttaatcaccc accacagcgc caggacgttg aatttgcagg
     3001 attacagcat tgccgccgga aacagcgcca acctgattat cctgccggct gaaaatggat
     3061 ttgatgcgct gcgccgtcag gttccggtac gttattcggt acgtggcggc aaggtgattg
     3121 ccagcacaca accggcacaa accaccgtat atctggagca gccggaagcc atcgattaca
     3181 aacggtgatc ggctgggctg tgtgttgaca aagcgagcgt agtttatgcc ggatgcggct
     3241 tgaacgcctt atcaggccta cgtagagcac tgaactcgta ggcctgataa gcgtaacgca
     3301 tcaggcaatt gtgtcagtgg ttaccgcgat tcattctcgc caacatccgc acattcctcc
     3361 aacgccatgt gcaaaaatgc cttcgcggcg gctgtctgcc agctattttt ccgccgcaac
     3421 aaaaccgccg ttctctccag tagtggcggg gcaagaggaa tcgctttaag cccgtcatgt
     3481 tgtgtggcaa tcgctgctgg taacaatgtg gaaagggaag tgcggcgaat cagctccaga
     3541 accgcgctaa ttgagttcgc ctcaatgacc acctgtggat gcagccccgc tttctcgcag
     3601 tagtggtcaa tctgctctct ggtggcaaat tccgcgctga gcaggaccag tttttcatca
     3661 tgcaagcgac tcaacgccac ctgttcacag gcagccagcg gatgatgttg cgccacgact
     3721 aacgctaaac tttctgtcag taaaggaatt gcctccagct ccggcaaatg cacaggcgcg
     3781 aaggcaatcc caacgtccag ctcgtcgcga caaagcagat cttcgatttt ctcctgcgac
     3841 atttcctgta gctggagcgt gatgccgggg tagcgcgcat agaaatccgc cattaagggg
     3901 ccgataaagt agctcgtaaa ggtgggggtg acggcgatac gcagcgatcc tctcgtcaga
     3961 tcggcaacat catgaatcgc ccgtttaccc gctcccagtt cctgtaacgc ccggctggcg
     4021 tactgtcgcc agacttctcc tgcatcagtg agatgaatcg ttcgcccgct acggtcaaac
     4081 agcggcacgc ctaaactctc ctctaactgg cgaatctgct gggaaagcgc aggctgagag
     4141 acgtgcaacg cactggcggc acgggtgaag ctgccatgtt cagccacggc aagaaaataa
     4201 ttgatatgtc gagagagcat tcgccaacct ataagtaaat tcaatagaac tcatcataaa
     4261 tgagactttt accttatgac aatcggcgag tagtctgcct ctcattccag agacagatag
     4321 aggttaacgg tgaaagagat tattgatgga ttccttaaat tccagcgcga ggcatttccg
     4381 aagcgggaag ccttatttaa acagttggcg acacagcaaa gcccgcgcac actttttatc
     4441 tcctgctccg acagccgtct ggtccctgag ctggtgacgc aacgtgagcc tggcgatctt
     4501 ttcgttattc gcaatgcggg caatatcgtc ccttcctacg ggccggaacc cggtggcgtt
     4561 tctgcttcgg tggagtatgc cgtcgctgcg cttcgggtat ctgacattgt gatttgcggt
     4621 cattccaact gtggcgcgat gaccgccatt gccagctgtc agtgcatgga ccatatgcct
     4681 gccgtctccc actggctgcg ttatgccgat tcagcccgcg tcgttaatga ggcgcgcccg
     4741 cattccgatt taccgtcaaa agctgcggcg atggtacgtg aaaacgtcat tgctcagttg
     4801 gctaatttgc aaactcatcc atcggtgcgc ctggcgctcg aagaggggcg gatcgccctg
     4861 cacggctggg tctacgacat tgaaagcggc agcatcgcag cgtttgacgg cgcaacccgc
     4921 cagtttgtgc cactggcagc taatcctcgc gtttgtgcca taccgctacg ccaaccgacc
     4981 gcagcgtaac cttattttta aaccatcagg agatgcacca tgattcagtc gcaaattaac
     5041 cgcaatattc gtcttgatct tgccgatgcc attttgctca gcaaagctaa aaaagatctc
     5101 tcatttgccg agattgccga cggcaccggg ctggcagaag cctttgtgac cgcggctttg
     5161 ctgggtcagc aggcgcttcc tgccgacgcc gcccgccagg tcggggcgaa gctggatctc
     5221 gacgaagacg ccattctact gttacagatg atcccactgc gtggctgcat tgatgaccgt
     5281 attccaaccg acccaacgat gtatcgtttc tatgaaatgt tgcaggtgta cggcacaacc
     5341 ctgaaagcgt tggttcatga gaaatttggc gatggcatta tcagcgcgat taacttcaaa
     5401 ctcgacgtta agaaagtggc ggacccggaa ggtggcgaac gtgcggtcat caccttagat
     5461 ggtaaatatc tgccgaccaa accgttctga cagccatgcg taaccatcaa aagacgttca
     5521 cgatgctgct ggttctggtg ctgattggtc ttaatatgcg accactgctc acctccgtcg
     5581 ggccactgct accgcaattg cgccaggcaa gcggaatgag ctttagcgtg gctgccctgt
     5641 tgaccgctct gccggtggtt accatgggcg ggctggcgct ggccggaagc tggcttcatc
     5701 agcatgtcag cgaacgtcgc agcgtcgcca tcagtttgtt actgattgcc gtcggtgcat
     5761 tgatgcgtga gctttaccct caaagtgcgc tgctgcttag cagcgcactg ctcggtgggg
     5821 tggggatcgg catcattcag gcggtgatgc cttcggtgat taaacggcgg tttcagcagc
     5881 gcacgccact ggtgatgggg ctgtggtccg cggctctgat gggcggcggt gggcttggtg
     5941 ccgccctgac accctggtta gttcaacata gcgaaacctg gtatcaaaca ctcgcctggt
     6001 gggcgctgcc tgccgttgtt gcgctctttg cctggtggtg gcaaagcacc cgcgaggtcg
     6061 cctcttccca caagacaaca accattccgg ttcgcgtggt tttcactcct cgcgcgtgga
     6121 cgctgggtgt ttacttcggt ctgattaacg gtggttacgc cagcctgatt gcctggttac
     6181 ccgccttcta tattgagatt ggtgccagcg cgcagtacag cggttccttg ctggcattga
     6241 tgacgcttgg gcaagccgca ggagctttgc tgatgcctgc tatggctcgc catcaggatc
     6301 ggcgcaaact gttaatgctg gcgctggcgt tacaactggt gggtttctgc ggctttatct
     6361 ggctgccgct gcaattgccg gtattgtggg cgatggtgtg tggtttaggt ctgggcggcg
     6421 catttccgct ctgtttgctg ctggcgctcg atcactctgt gcaaccggct attgctggca
     6481 agctggtggc gtttatgcag ggaatcggtt ttatcatcgc cgggcttgcc ccgtggtttt
     6541 ctggcgtgct gcgtagtatc agcggcaatt acttgatgga ctgggcgttt catgcgctgt
     6601 gcgtcgtagg gctgatgatt ataaccctgc gttttgcacc agcacgtttt ccgcagctgt
     6661 gggtcaaaga ggcatgatgc gacgcttgtt tctgcgcttt gttcacgccg gatgcggcct
     6721 acatagatca ctgaatttgt aggcctgata agcgaagcgt atcaggcaat ttttataatt
     6781 taaactgacg attc
//
LOCUS       DAZFKY010000223         6783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFKY010000223 DAZFKY010000000
VERSION     DAZFKY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..765)
                     /locus_tag="ACHKCL_004255"
     CDS             complement(<1..765)
                     /locus_tag="ACHKCL_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO5765099.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQF"
     gene            complement(774..1058)
                     /locus_tag="ACHKCL_004256"
     CDS             complement(774..1058)
                     /locus_tag="ACHKCL_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO5765100.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            1336..3768
                     /gene="hsdR"
                     /locus_tag="ACHKCL_004257"
     CDS             1336..3768
                     /gene="hsdR"
                     /locus_tag="ACHKCL_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO5765101.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3835..5304
                     /locus_tag="ACHKCL_004258"
     CDS             3835..5304
                     /locus_tag="ACHKCL_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5765102.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            5304..>6783
                     /locus_tag="ACHKCL_004259"
     CDS             5304..>6783
                     /locus_tag="ACHKCL_004259"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5765103.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQD"
BASE COUNT         2048 a         1550 c         1504 g         1681 t
ORIGIN      
        1 aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc
       61 cataaccgga ggaatccact catctacccc taaattactt agcgatgcta tttttcgtcg
      121 tattgaaggg tgttcaaaat cattctctaa aatcttagta taattttttg cagaattaac
      181 caacatcagt gacatagcaa tagcatctcc atcaaattgc tgctggagat tttcaataaa
      241 agcttcaaaa cctttttgta acaccctgtc acgatctttt ttctcggaaa gcttatgaag
      301 agtaagaaat ttatcaagcc gtctcactcc gaccatatct tcaatttgcg accatgctga
      361 ttctatttgc tctgattttt tattatgtgt agaagctgat tcaaacagtg cattttttaa
      421 aagatctgca ttacttaaag gtaaacctcg actatttaat acattaaaga gtctaaatga
      481 tgatgcgaaa tcatctgttt gcacaaatac gatatataca ttttgcaaaa tatatttagc
      541 aagcaacttc aactcatcaa cagataaccg caacaaataa tctctaattg tttcagcgtt
      601 gctaatgaat actaactcag tatccgatgg cttttcaggt ttataatctt tagaatcctg
      661 cagtatataa tatttataaa gatcatgctc tttttttcta acaattagtc ttggttcatc
      721 tgtttcatca gaataaacat caataggcag aactcttttt tgcaaatcat cttttatccc
      781 ctgaatcggg atgatttttt tagctcggag acaataatgc ttaacgttgt aagacgttgt
      841 tgtccatcta caacttcata ctgattttgc cctttattga tacaaatcat actcccaata
      901 aaatatccat tctcttctga ttgagagctt ttataaatgt catcaatgag ttgctctgca
      961 ttatctaccg tccaactgta aggacgctga taagaaggaa taataaactt tttgttttca
     1021 gtaaggatat cctgcacctt acattcttta gcttccattt catataccca atcaaacaat
     1081 caacaataac attatctttc ttgctcgaaa gaaacgcaac tataagattt ttatatattt
     1141 tgttagagtt gatatggttc ctgtaacttc tgtttatctg tctctgatat gcccagatta
     1201 attcaagcaa gatataacag agatatcaat gtgatctctg ctacgtactt tacgttgaaa
     1261 caccgcccta aatcatagcc aggctattga ttttatgtac aatcagaatc gtcctgataa
     1321 aagcgagaat accccatggc ggaactgaac ctaagtaacc tgacggaagc agacatcatt
     1381 accaaatgcg ttatgccagc cattctcaat gcaggctggg acaacacaac gcaaatcaga
     1441 caggaggtca aactccggga cggtaaagtc atcgtacgtg gtaaagttgc ggcacgcaga
     1501 acggtaaaat ctgctgacat cgtgctgtat cacaaacctg gcattccctt agccgtgatt
     1561 gaagcaaaag ccaacaaaca tgaaattggc aaagggatgc aacagggcat tgaatatgcg
     1621 cgcctgctgg acgttccttt tgtttttgcc accaacggtg atggctttat cttccgcgat
     1681 gccaccgcag ccgaaggtga atgccttgaa aagcaaataa cgctggatga cttcccctcc
     1741 cccgctgaac tctggcaaaa attctgcctc tggaaaggat acacacaagc tcagcttccg
     1801 gtgattactc aggactatta cgacgatggc agcggtaaat cgccacgtta ttaccagctt
     1861 caggcaatca acaaaaccat tgaagccgtc tccaacgggc aaaaccgcgt tctgctggtc
     1921 atggcgaccg gaacagggaa aacctatacc gcattccaga tcatctggcg cctgtggaaa
     1981 tcaaaaaata aaaaacgcat tttgttcctt gccgatcgca atattctggt cgaccaaacc
     2041 aaaaataatg atttccagcc atttggtacg gcaatgacca aagtcagcgg acgcaccatt
     2101 gatcccgctt atgaaattca cctcgcgctc tatcaggcta taactggccc ggaggaagac
     2161 caaaaagcgt ttaaacaagt cgcaccagat ttcttcgatc tgatcgtgat cgacgaatgc
     2221 catcgcggca gcgcatccga agacagcgcc tggcgagaaa tccttgatta ttttagttcc
     2281 gccacccaaa ttggcttaac cgccacgcca aaagagacgc atgaagtctc cagcacggat
     2341 tacttcggcg atccggttta cgtctactca ctaaaagaag ggatcgaaga cggcttcctc
     2401 gccccttata aagttgtccg tgttgatatt gatgttgatc tgcaaggctg gcgcccaacc
     2461 aaagggcaaa ctgacttaaa cggcgaagtg atcgacgatc gtatctataa ccagaaagat
     2521 ttcgatcgca cgatggtaat cgacgaacgc acagaactgg ttgccagaac cattaccgac
     2581 tacctcaagc gtaccaatcc gatggataaa accatcgtct tctgtaacga catcgatcat
     2641 gcagaacgta tgcgccgcgc cctggttaat ctcaacccgg agcaggtgaa aaagaacgac
     2701 aaatacgtca tgaaaatcac cggcgatgat gaaattggca aagctcagtt ggataacttc
     2761 atcaacccga aaaaaccgta cccggttatc gcgaccactt cagagctgat gaccaccggt
     2821 gtggatgcta aaacctgcaa actggtagta ctggaccaga acatccagtc gatgaccaaa
     2881 ttcaagcaga ttatcggtcg tggtacacgc atcgacgaac gttacggcaa actctggttt
     2941 accatcctcg actttaaaaa agccaccgaa ctgtttgccg atgagcgttt cgatggcatt
     3001 cccgaaaaag tcatggatac cacaccagag gatatcgccg atccagaatc tgattttgaa
     3061 gagaaactcg aagaaatcag cgaacatgac gacgaacagg taacaggcgt tgatgaaccg
     3121 cctgcgccac cataccaggt taaagatacc gatgatgtcg gcccacttcc ggaagaagac
     3181 gagaagaaaa tccgcaagtt tcacgtcaac ggtgtagcag tgggcgttat tgcccagcgt
     3241 gttcagtatt acgacgccga cggtaaactg gttaccgaat cctttaaaga ttacacccgc
     3301 aaaacactgc tcaaagaata tgcctcgctg gatgacttta cccgcaagtg gcaggacgcc
     3361 gatcgcaaag aagcgatcat tcacgagctg gagcaacagg ggatcatctg ggaagtactg
     3421 gcagaagaag tcggtaaaga tctcgacccg ttcgacatgc tttgccacgt agtgtatggt
     3481 cagccgccgt taacccgcaa agagcgcgcc gagaacgtgc gcaagcggaa ctacttcaca
     3541 aaatactctg aagcagcgca agccgtgctc gataatctgc tggataaata cgccgatgcg
     3601 ggcgtacagg agatcgaaag tattcaggtg ctgaaactta agccattcga cagcatgggc
     3661 accttaccgg agattattaa aaccggattt ggcgaccgta acgggtataa tcaggcgctc
     3721 agcgagctgg aaaacgaaat ctaccaatta ccgccccgct ctgcttaaca actctctttt
     3781 gcgccttctg caaccatcat gttgcagaag gccttgtttt tcatggaata caacatgtct
     3841 atcagctcag taatcaaatc attacaagat attatgcgca aagatgccgg tgtggatggc
     3901 gatgcgcagc gtctcggtca gctctcctgg ctgctgtttt tgaaaatctt cgatgcccag
     3961 gaagaggcgc tggaactgga gcaggataac tatcaatatc cgatcccaca gcgttattta
     4021 tggcgcagtt gggccgccaa cgcgcagggc attaccggtg attccctgct ggaattcgtt
     4081 aatgatgatc tgttcccggc gttgaaaaac ctcactgcgc ctatcgataa aaacccacgc
     4141 ggctacgtgg tgaagcaggc gttcagcgat gcctataact atatgaaaaa cggtacgcta
     4201 ctgcgtcagg tgatcaataa gctgaacgaa attgacttta ccagcgccag cgaacgccat
     4261 ctgtttggtg atatttacga acagatcctt aaagatctgc aatctgcggg caatgcgggc
     4321 gaattctata ctccacgcgc cgtcactcgc tttatggtgg atcgcgttga tccgaaactc
     4381 ggcgaatcca ttatggaccc ggcctgcggt acgggcggtt ttcttgcctg cgcatttgat
     4441 catgtaaaga acaaatacgt gaagagcgtc gccgatcatc agacgctgca acaacagatc
     4501 cacggtgttg agaaaaaaca gcttccgcac ctgctggcga ccaccaatat gctgctacac
     4561 ggcattgaag tgccagtgca aattcgtcac gacaacactc tgaacaaacc gctttcctcc
     4621 cgggatgagc aactggatgt cattgttacc aacccgccgt ttggtggcac ggaagaagac
     4681 ggtattgaga agaactttcc ggcagagatg caaacccgcg aaacggcgga tttgttcctg
     4741 caactgattg tggaagtact ggcgaaaaat ggtcgcgcgg cggtggtatt gccggatggc
     4801 acactatttg gcgaaggcgt taaaaccaaa atcaaaaagc tacttaccga agagtgcaac
     4861 ctgcatacca tcgtgcgctt accgaatggt gtgtttaacc cctataccgg cattaaaacc
     4921 aacctgttgt tctttaccaa aggtcagcca accaaagaga tttggttcta tgagcatccg
     4981 tatccggcgg gcgtaaaaaa ctacagcaaa actaagccga tgaagtttga agagtttcag
     5041 gcggagatcg actggtgggg taacgaggcc gatggttttg ccagccgcgt agagaatgag
     5101 caggcgtgga aagtcagcat tgatgacgtg attgcgcgta acttcaatct ggatattaaa
     5161 aacccacatc aggcggaaac cgtcagccat gatccggccg agctgttagc gcagtatgca
     5221 aaacagcagg cggagatcca gacgctgcgt aatcaactgc gcgatattct tggcgctgcg
     5281 ctgtctggca aggaggttaa ctaatgagtg tggaaaagct gatcgttgat catatggaaa
     5341 cctggacctc ggcgttgcaa acccgttcca ccgccgggcg cggcagttcc ggaaaaattg
     5401 atttatatgg cattaagaaa ttacgtgagc tgattctgga actggctgtg cgcggtaaac
     5461 tggtgccgca ggacccgaac gatgaaccgg cgtcggagct gctgaagcgt attgcggcag
     5521 aaaaagcaga gctggtgaag caaggtaaaa ttaaaaagca aaaaccactg ccggaaatta
     5581 gtgaggaaga gaagccgttt gaattgccgg agggatggga gtgggtgcgt attagtgaaa
     5641 ttggacatga ctggggacaa aaaactccag ataaagattt tacttacatt gatgttggtt
     5701 caataaataa agaatatgga attattgaag agctatctat tctttcagcg aaagatgccc
     5761 catcgcgagc gagaaaaatt gttcagcaag ggactatcat atactctact gttcgcccgt
     5821 atttgttaaa tatagctatt attgaaaacg aaatactacc tgaaccgatc gctagtactg
     5881 ccttcgctat tattcatcca tatacggcga tggatgctaa tttcatttat tattatctac
     5941 gttctccggt atttgtttgt tatgttgaaa attgtcagac aggggttgcc tatccagcaa
     6001 tcaatgacaa acaatttttt tctggaataa ctccagttcc tccatccttg gaacaagttc
     6061 gcatcgcaaa caaaatcaaa gaattaatgt ccctctgcga ccaactggaa cagcaatccc
     6121 tgaccagtct ggacgcacat caacaactgg ttgaaaccct gttgggaacg cttacagaca
     6181 gccaaaccgc cgaggaactg gctgaaaact gggcgcgtat tagcgagtat ttcgacactc
     6241 tatttaccac cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa
     6301 tgggcaaact tgtgccgcag gatccgaatg atgaaccagc ctctgaactg ctcaaacgaa
     6361 ttgcgcagga aaaagctcaa ctggtgaaag aaggaaaaat aaaaaaacaa aaaccgttgc
     6421 cgccaattag cgatgaggaa aaaccgtttg aacttccgga agggtgggag tggtgtttat
     6481 ttgaagatat tattgatatt caaagtggta tcactaaagg aagaaattta tcaaatagaa
     6541 ctttggtaaa agttccttat ttacgtgttg caaacgtcca acgcggatat cttgatctta
     6601 cggaaattaa acagattgaa atccctattg aggaaaaaga aaaatatcaa gtagtcaagg
     6661 gagatttatt gataacagaa ggcggcgact gggatacagt cgggagaact acagtatggt
     6721 gtcatgactg gtatatagca aatcaaaacc atgtattcaa aggacgaaat atagggcaag
     6781 atg
//
LOCUS       DAZFKY010000224         6726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFKY010000224 DAZFKY010000000
VERSION     DAZFKY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..406
                     /locus_tag="ACHKCL_004260"
     CDS             <1..406
                     /locus_tag="ACHKCL_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFO5765104.1"
                     /translation="TLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTW
                     DRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
                     DLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(414..680)
                     /locus_tag="ACHKCL_004261"
                     /pseudo
     CDS             complement(414..680)
                     /locus_tag="ACHKCL_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            838..2022
                     /locus_tag="ACHKCL_004262"
     CDS             838..2022
                     /locus_tag="ACHKCL_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5765105.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2022..2534
                     /locus_tag="ACHKCL_004263"
     CDS             2022..2534
                     /locus_tag="ACHKCL_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5765106.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2589..2954
                     /locus_tag="ACHKCL_004264"
     CDS             2589..2954
                     /locus_tag="ACHKCL_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765107.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2963..3118
                     /locus_tag="ACHKCL_004265"
     CDS             2963..3118
                     /locus_tag="ACHKCL_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5765108.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3105..5908
                     /locus_tag="ACHKCL_004266"
                     /pseudo
     CDS             3105..5908
                     /locus_tag="ACHKCL_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5921..6409
                     /locus_tag="ACHKCL_004267"
     CDS             5921..6409
                     /locus_tag="ACHKCL_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765109.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6566..>6726
                     /locus_tag="ACHKCL_004268"
     CDS             6566..>6726
                     /locus_tag="ACHKCL_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO5765110.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWR"
BASE COUNT         1627 a         1609 c         1936 g         1554 t
ORIGIN      
        1 cacacttgta gaccgaaaat cacgttatac gatcatcctc agactcaggg gcaaagattc
       61 tgtctcagta aatcaggctc ttaccgacaa attcctgagt ttaccgtcag aactcagaaa
      121 atcactgaca tgggacagag gaatggaact ggccagacat ctagaattta ctgtcagcac
      181 cggcgttaaa gtttacttct gcgatcctca gagtccttgg cagcggggaa caaatgagaa
      241 cacaaatggg ctaattcggc agtactttcc taaaaagaca tgtcttgccc aatatactca
      301 acatgaagat ctggttgctg ctcagctaaa caacagaccg agaaagacac tgaagttcaa
      361 aacaccgaaa gagataattg aaaggggtgt tgcattgaca gattgaatct acaagtgcct
      421 ttttctgtca gcggttctcc tttccagcca atgagaaccg tcaggcgcgc gccccgtggc
      481 ggtagctgca actgaccatc tgcatcatcc agcgtgatgg tgagctggtc cgcctcaaat
      541 ccccggttgt cggtcagtga caggctcatc aggcgctctg ccacgcctga cagcgtttta
      601 ccctccgcga gaatatcaaa atccggcact ttcacgtggt ctgtgccctg actgagcaat
      661 tgcatggtgg tgtcggtcat ctgttccctc cctgtgcggc atggtcgcat gtgcgtgcgg
      721 agggggttac tgctttttgt tgtcgccgtg gcgggagaac ggcgcagggg tgagattacg
      781 cgcgtggtgg gtgatgattg ttgccgaatc atttaacgga tacaaggggc tgaagctatg
      841 agtgaaactc gttttcatgg tgcccgtgtt acggaaagta ccgacctggt aacagcgatt
      901 aatgatgttg attccagtgt tatcggtatc gtggcaacgg cagatgatgc ggacgcggag
      961 ctgttcccgc tgaacaagcc cacactgctg acccgcgtca atgacgtgct gggaaaatgc
     1021 gggactacgg ggacgcttta tcgtgcgctt aaggctatcg cagaccaggt gagcacaaaa
     1081 gtgatcgtcg ttcgcgtggc tgaacacaaa gaagaaggcg gtaagacgca ggatcaactg
     1141 gttatcggtg gttctgaatc tgacggcagc tatacgggga tgtatgcgct gcttgttgca
     1201 gagcaggatg aaagcatcgg ataccgtccg cgtattctgg ccgcgccgga gctggacacg
     1261 gaggcggtaa caaaatccct gtgcgtgatt gcgggtaaac tgcgcgcgtt tgtgtatgcc
     1321 acatgtcatg gttgtaacac gatggctgag gcgattacct accgccagaa attcaacgaa
     1381 cgtgaggtga tgctcttatg gcctgacttc atcgcctaca acctgaaaag tggcaaaaac
     1441 gaaacgttcc ccgcgcctgc ttatgcgtgc ggccttcgtg cgtacattga ccatgagcag
     1501 ggctggcaca aatcgctgtc caacgttccg gttaaaaatg tgcttgggat gtcgaggcat
     1561 gtgttctggt cgttgcaggc cgaagacagt gatgccaaca gcctcaacaa caaagaaatc
     1621 acgaccatta ttcgtcgcaa cgggttccgc ttctggggca accgcacacc ggaaacgaac
     1681 gcctacatct ttgaggtgta tacccgaacc gcacaggtgc tggctgattc aattgcggaa
     1741 gcgcagtttg aaaccatcga cagtccactg acacctgcga acgtgaagga tgttatcagt
     1801 gccatcaggg caaaactgga ttcactggtt actgccggga aactgattgg cgcggagtgc
     1861 tggtatgaca tcgaggataa cagcaccacg aatttacgtc aggggcgtgt gcgtattcgc
     1921 tacaaatata cgcccgttcc tccgctggaa gacatggagc tttaccagtc gtttactgat
     1981 gaattctttg gtcccgcatt tgcggtgctg ggaggtgcct gatggctgta ccaaaacatc
     2041 ttcgcttttt tacgctgttt gtggatggtg aaaacgaagt gggtaaggtg acgtccgtca
     2101 ccctgcctaa actgacgcgc aaaaccgaca gctaccgggg tggtggcatg atgggtgcgg
     2161 taagtattga cctcggcctg gacgactccg cgcttgatgc gagctttgtc atggggggcg
     2221 cagttcgtga gctgttcctg aagtatggcg gcacgattga cggcacgcta ctgcgttttg
     2281 cgggggaata ctacaccgat gatgaaagcg acctgtatga agtcgaaatg cgcggacgtg
     2341 tgacggaaat tgatatgggg gaagccaaac agggcgaagc cacatcacac acttacgcca
     2401 ttaaaaacac ctactacaag ctgagtgtta acgatcgccc gttgtgggag attgacctgc
     2461 tgaacttcat ttaccggaag gacggcaagg acattgtgcc cgatcgcatc cgttccgcgc
     2521 ttgggcttgg ctgataagta atatgcaggc ggcgcagtgc gtcgcctctg actgaaagga
     2581 gtttcctgat gaaagagacg aaaaacatcg ataccgaaaa cacggtagtt actgacactg
     2641 tgaaagaaac cagtgagcgt ggtgtaaaac ttacccaacc aattgagcga ggcggcgaaa
     2701 aaatcacgta tgtggagatc accggggcta ttgagcaggc tggatctctg cgagatttgt
     2761 cgctgtctga tgtgctgaat ctgaaagcgg aatccatgtt tacgctgctg tcacgcgtga
     2821 catcaccgcg actggatgaa gtgacgatca aaaaaatggc atcccgtgac tttattcagt
     2881 tatgtgtggt tgccgtaaat tttttgagcg gtgcggactc tggcgggaag aacgaacagg
     2941 cgacggaagc ctgatcacgg ttgtgtgctt tgagcacata gaagactttg tggcagatat
     3001 tgccgttatt tttaactggt cgcccgccga aatcttcatg atgacgcccg gcgaagtggt
     3061 tagctggcgt gagcgggcgg cacttcgcag cgggaatgca gacaatgaag actcttgata
     3121 tccgggtcgc tttcagcgcc gttgacaggc tgacccgacc tgccgaaaac gcacgccgcc
     3181 tgatggggca gtttggtgac tccatccagc gaacgcaggg ggcgatcaaa aatctcgagc
     3241 gtcaggcgcg atcatttgag cgcgcccgcg acgctgtcag taaagcggat gctggcatcg
     3301 tgaaagcacg acgccagctt aacgccctta atcagttaca acgcacgggt acagtgctca
     3361 gcgaaaaaca acaaaagctg atgcagcagt taagcacccg gcttgaacgc ctgaatgaat
     3421 cgcgcacacg ggaaattcag aaaatgaggg aacttggcgg agagctgaaa cgccacggca
     3481 tttccctgac aggcagcgat aacaccattc agcaggccat cagacgcacc gaacaataca
     3541 acaaccagct tgaacgcgaa cggcaggcgc ttgcgcgtgt aacgcaggcg cgtgagcggt
     3601 attcgcacgc gcaggaaacc gcgggaaaac tgaaaacagg tggtgcgctg gcaattggtg
     3661 cggcagcggc gggcggctat gctgccgggc gttttttgca gcctgcgatc gggttcggca
     3721 aagagatgtc ccgcgttcag gcactgacgc gaatcgacaa aaacagcccg cagtttaagg
     3781 cgctgcgtga gcaggcgtta aaacttgctg aaacacagtt tactgcgagt gatgccgcca
     3841 gtgggcagag ctttctggca atggctggtt ttactccgca ggccattcag gccgcattgc
     3901 ccggtgttct taatatggcg ctggcaggtg gcgtcgaact cggcgagacg gctgatatag
     3961 gctccaatat cctcacacag ttcaacctga cagccgatca aatggaccgg gtgggcgata
     4021 cgctgacagc agcatttacc cggaccaata ctgatttacg cgcgctgggc gaaaccatga
     4081 agtataccgg tccggttgcc gcaaaacttg gtatcagtct tgaagaagcg gcggccatgg
     4141 ccgggatgct tgccaataat ggtcttcgcg gaagcgatgc tggcacggcc atgcgcgcaa
     4201 gtctgtcccg ccttgcatca ccgccaaaag ctgcggctga tgcactgaaa gagctggggg
     4261 tgtcagttgc tgacgccaga ggcaaaatgc gcccgatgga ggatgtgctg cttgatctct
     4321 ataaggcgac acaaaaatac ggacaggtgg accaggtctc cttcttcaag gacatcgccg
     4381 gagaagaggc gttcgttggt ttgcagacgc ttgttgcggc ggctggttca ggagagctgc
     4441 aaaaactgac cagagaattg cagggggcaa ggggagaggc cgatcgcgtc gcaaaagtaa
     4501 tggctgataa tcttgatggg gacctgaaaa atctcgacag cgcatgggaa ggtcttcgta
     4561 ttcgcatcag tgatctggtt gacggtccgc tgcgttctgt cacgcagtgg ctcacgcggg
     4621 tgcttgaaaa aatcacctcg ctggcgcagg cccatccggt actgacgcgc cagctactga
     4681 tagcaggcgg tgcgttgctg gcaatgactg caacgattgg ctcgttgtcg ctggttattg
     4741 gggtgcttta cgggaagctg gccaccctgc gtcttggttt tgacattctt acccggtcaa
     4801 tgaatgtcgt cagggtgtta cctgcgctat ggggaatggt gacgggttcc gtttctttac
     4861 tgggaggcgt tatcggggcg ttgttcagtc cggttggtct tatcgtggct gcgcttgccg
     4921 gagctgccgt tcttatctgg aaatactggg atcctatcag ggctttcttt gccggggtgt
     4981 tcagcgggat tatggaaagg ctgaccccgt tgcgcgacac ctttgaacgg tttggccctg
     5041 tttttgacgt aatcggaagc ggttcagcca ggtgtttaac tggtttaaat cgctgctgtc
     5101 accgatggag tccagcaagg aaacgctgga taaatgtacc agtgctggcg aggtattcgg
     5161 taacgttctt ggtggtgcgt tacagcttgt tctgacgcct gcaaaaatgt tgctggatac
     5221 gctggcgtgg atacttgaaa aactcggtgt gcttccggat gaagcggaaa gggcgcgcaa
     5281 gaaaatcgaa gacgcacaac gtgcggccat tcttcaggac aaggttgctc tgtttcaggg
     5341 agacattgcg aaaatcaatc cgccgaagtc tgcgggaaat agcaatggca ccggagatga
     5401 taaacccaaa gacaacaaac ctctcacaga cagcaatacc ggcacgctac gcagactcag
     5461 caaaattgct gataacacag gtaagctggt tgatgagaca aaaaaacgca ttggccccgg
     5521 cgatattgtc tttaagaacc tgccccgcgc acttgctgtt cgtggggagt ggcaggagcg
     5581 gaagattgcg caggtcagta agcctgcccc cgcaattaat atcacacccg tggtcccggc
     5641 tccgctgcct ccggcgctgg tccctgttgt tgcggccagc tcccgcccgg tggtggaggc
     5701 catacgatcg ccagtggcat cagttcctgc aacttcccgt aaccgggagc ctgttgcctc
     5761 cggatttggc ggtgaaattc atgttcatct gcataacgtt gttacgcaga atccccgcga
     5821 actggcgaaa ctggtcggcg aaatggtcag ggcagaaatg gaacggcgcg cccgtgccag
     5881 gcgtggcagt ttttacgata aagattgagg agtcatggcc atgatgatga tctacggcat
     5941 gtttgttttt gagctgcgca cgttgccgca tcagcagtta cagcaaaaca aaagctggcg
     6001 acatgtgaaa aatgaacgcg ttaaccgttc agcaagctgg cagtatatcg gtgcaggtga
     6061 tgatcgcatc gttctttctg gtgttcttta tcctgagatt actggtggcg aagtgtcgct
     6121 gtcgttgttg accacgcagg catatacagg acgtccttgg cctttgattg atggcgtcgg
     6181 gcagatttac ggcatgtatg tcctgaccgg aacgaatacg acccgctccg agtttgatcg
     6241 ctacggtaag gcgaaaaaga tagaattttc actgactctt gaacgctgtg atgaggattt
     6301 gcgggagcgc ctgcaatcct catcgttcag cgatatgctg tccggcttca aagataaggt
     6361 gacatcatcc cttaatagcg cggccagctc agttaagggg ctgctctgat ttaacgtatg
     6421 tcgccaattt cctgatgaag gtgactggcg acttgctgtt gtatgtcctt ctcagaaaat
     6481 tgtttttgaa taacaaataa caggatttta taatctctta accttataac atgtgtggtc
     6541 tgaaataata attaaggaga ttatcgtgct atcttactta atggcaattc actttgtttt
     6601 atttggaaac tctactaatt taaaaaactt ctggaaacat gaagtaattc ggcggaaacg
     6661 tatggatatc tggaggcttt taagagagaa aaagcagcgt aaccggaatt tccttttctg
     6721 gtggcg
//
LOCUS       DAZFKY010000225         6715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFKY010000225 DAZFKY010000000
VERSION     DAZFKY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..278
                     /locus_tag="ACHKCL_004269"
     CDS             <1..278
                     /locus_tag="ACHKCL_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFO5765111.1"
                     /translation="KKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDS
                     EGVDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
     gene            complement(506..664)
                     /locus_tag="ACHKCL_004270"
     CDS             complement(506..664)
                     /locus_tag="ACHKCL_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5765112.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(1324..1515)
                     /locus_tag="ACHKCL_004271"
     CDS             complement(1324..1515)
                     /locus_tag="ACHKCL_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5765113.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGEDL"
     gene            1580..1903
                     /locus_tag="ACHKCL_004272"
     CDS             1580..1903
                     /locus_tag="ACHKCL_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO5765114.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQG
                     ARDDC"
     gene            2112..2543
                     /locus_tag="ACHKCL_004273"
                     /pseudo
     CDS             2112..2543
                     /locus_tag="ACHKCL_004273"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2581..3099
                     /locus_tag="ACHKCL_004274"
     CDS             2581..3099
                     /locus_tag="ACHKCL_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO5765115.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVT
                     HYQGKDWIQLTE"
     gene            3106..3339
                     /locus_tag="ACHKCL_004275"
     CDS             3106..3339
                     /locus_tag="ACHKCL_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO5765116.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENWR"
     gene            complement(3714..3920)
                     /locus_tag="ACHKCL_004276"
     CDS             complement(3714..3920)
                     /locus_tag="ACHKCL_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765117.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQ
                     SGDGKLMATYRRNKKAIDKLDAEG"
     gene            complement(3913..4296)
                     /locus_tag="ACHKCL_004277"
     CDS             complement(3913..4296)
                     /locus_tag="ACHKCL_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765118.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKEL
                     ISKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINH
                     KISDTEDAVLSVGKIINLIEDSQHG"
     gene            complement(4687..5748)
                     /locus_tag="ACHKCL_004278"
     CDS             complement(4687..5748)
                     /locus_tag="ACHKCL_004278"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HFO5765119.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMN
                     PIYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVI
                     KGNNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHE
                     IRKIFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKS
                     PILDQNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNI
                     NRIYTEMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPE
                     RRFDQSVYYTASHLFDEIK"
     gene            5836..>6715
                     /locus_tag="ACHKCL_004279"
     CDS             5836..>6715
                     /locus_tag="ACHKCL_004279"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5765120.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSH
                     PIKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKR
                     IKPKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGK
                     LDVRDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTV
                     RGVNRPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGN
                     AVPVELAKYVAVTIMEY"
BASE COUNT         1768 a         1310 c         1751 g         1886 t
ORIGIN      
        1 aaaaaaaaca agtaaaaggt atttatgata aagaaggctt tagggcgtgg ttgctgaatg
       61 aaaagaaact aacaaaaaga acctctagcg atattatatc tagatgttgc agaggtgttt
      121 cattttttga ttcagagggg gtggattttt ataactgtga aatagatgaa attattatga
      181 aactggaaag gcttgaatcg tttgtcaggc ttggggttag cctgaagtct cagttaagaa
      241 gggcttttaa attatattac gaatattgtc gaaggtaata aatacgaatt tatgtctctc
      301 aggggagggg gaggggataa aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc
      361 gtaatctgct gcctgtaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa
      421 tctagagcta ccaactcttt cttccgaagg taactggctt ggtggagcgc agataccaaa
      481 tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa
      541 tccaccttgt ccagttacca gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg
      901 ggcggagcct atggaaaaac gccagcggcg cggccttttg ttgtcctttg cctttttgct
      961 cacatgttct ttccggcctt atcccctgat tctgtggata accgtattac cgcctttgag
     1021 tgagccgact ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1141 atatggtgct cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc
     1201 cgctatcgct acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg
     1261 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg ggagctgcat
     1321 gtgtcagagg tcttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     1381 ggcgtggtcg tgaagagatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag
     1441 tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     1501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     1561 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     1621 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     1681 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     1741 cgccatcggg aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc
     1801 cggtgaccgg gttcaggtgg tggccgcact gatggccatc gagggagagt tgcgcagctt
     1861 acggcaggtc gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa
     1921 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     1981 caacggtgct tcagggtaat ccggaagaag tcagggaact catcgatgcc acgccatttg
     2041 cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac
     2101 gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt
     2161 acagcattct gtgggtggag caccaggaca aggggcgtct tgagctgaat tttgtcattc
     2221 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccga gcagacaggc
     2281 cgagaattga tgcctggcag acactggtga atcaccatta cgggctgcat gaccccaacg
     2341 ccccggagaa ccgccggata ctgactctct ctgataacct gccggaaacg aaacaggcgc
     2401 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtaggggag ataaaaggcc
     2461 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     2521 gcagcatcag cattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt
     2581 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     2641 tctacagaga aaatgctgaa cgacgagttc aggaagctcg gcgagtctgt cagcgaggct
     2701 gtgacctcaa acgagacgaa aatcagagac gctatagccc tgttcacagc ctcgacagag
     2761 aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     2821 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatagtctt tctcctgttt
     2881 accgcgagtg gcgggacgct ctggtatctt ggcgggagga tacaggcgaa cctggaagaa
     2941 atcaggaagc aggaagagac attgcagaaa ctgaacgcga agacatgggg cgtggagttt
     3001 gtgcaggacg ggagaaggaa atttctggtc attccgcaag ggatgtcagc agaggtgacg
     3061 cactatcagg ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa
     3121 acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa
     3181 tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg
     3241 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcggaca
     3301 gaacgcttga gccagttata cagcgagaac tggagataag agaggaacgg ctgatacagg
     3361 agcgcgaaca tgcgttatcc ctggaacggg agcgtcagcc ggaaatacag gaacgcatac
     3421 tggatggtcc ttcgttgtcg ctgtgatggc gaaactataa aaaatggcag gttcagtgga
     3481 ttttgacgga ctaatgtagt ctgcaccatc tggttgcata ggtattcatg cggttaaaat
     3541 ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3601 gccgtgatcg caccgaacgc gttttagcgg tgtgcgtaac ggatgttatg gtaaatacaa
     3661 tatgcagcca gtcttagaga cgaaaagtcc gcccagaaga ctgacttttc agatcaacct
     3721 tcagcatcta acttgtctat cgctttttta ttgcgccgat aggtagccat tagtttacca
     3781 tcgcctgatt gtaagtcacg acctgcttgc tcagcatagg tatcgtttac agtcttagcg
     3841 ccttttttat aggccttttt aacatcatca aatccggctt ggatgacttc tttcggcttt
     3901 tcttgaggta atttagccat gctgtgaatc ctctataagg ttgattattt ttccaacaga
     3961 taagacggca tcttctgtgt cgctgatttt atgatttatc caaccgtcct ctatctttaa
     4021 tttgttcact actcctaatg taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt
     4081 tctacttttc tctttccaat cagagggcgg cgtcgaacgg aacgctacat tcctcgattt
     4141 aagtatcgct ttgattttac taattaattc ttttttttgt ttattgctga gatttttgac
     4201 gaaccctttg ctgagattgt ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt
     4261 ttgtttttta tcagatagat gaacaatgtt tgacactgga tatcctgctt tttgatgaat
     4321 gaatactcag aagagtagcg gaactgtctt gttagtcaca gaggtagagt atcaattcta
     4381 cgatagatgg gttcgaagat ccggctgaga ccgttcagta cgcggtcgag atgttatgtt
     4441 caatgtaatg accgtcgaaa ttgcctttac gggcatgaag caaccaggag caatcattgg
     4501 caaaggtaac tagagttcaa tgcatggtaa tcatttttga gcttgtgatg catagagttg
     4561 gttgctgttc cattgcgtct caaactccgt caagatgctc gccgtaaaag cgagcatgtc
     4621 tagtatgcct gttccgctac tgatttttga tgggcgaatt cagggctggc gagccaccaa
     4681 gaaaaattac tttatttcat caaacaggtg agatgcagta taataaacgc tttgatcaaa
     4741 gcgtctctct gggcttaatt tattttgccc gccatttaaa attatttctt ttaatactga
     4801 tggtgggagg atgggttttg ttcttcttgg gtgatccata taagcttttg ttatttttcc
     4861 gtttagcgag aactctttat cactttgata tccaaagttc atctcagtat aaattctatt
     4921 gatgtttgtc ttgtttttgc tcgcaatttc atacaccatt ctggcgagtc tggctgatga
     4981 tctcattagt ctgctggcag atgaattgtt atatgaggat ctatcaataa aggttcgaca
     5041 caaagcaaaa tcactccaga tgaatatgtc aaaggcattt tgatctaata ttgggctctt
     5101 gccttttgtt ttccataatg gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc
     5161 ttggaaagtg tttaggcaat ttaatatatt ttctttcttt gaaagaactt cagtttcatt
     5221 cccccagtgc tgtatgcttt cgcatgtgga ttcaaatatt tttcttattt catggaattt
     5281 atcgctgcaa gagtgcatta tgccaagggc acaataagaa gtgcttgcag gtcttattac
     5341 aatctctgaa ctccattcgc tctcgttttc tttatatgtc gtgctatctg gaagtacggt
     5401 taattttatt tctagtgctt ttaaaaaact attgtttcct tttataacta gatctattcc
     5461 ttttatgtca tcataagcat actgttggta tggttcatat tttttctcaa attgaaattc
     5521 aagatcacta ttttttaatg ttgtgttaaa aacgaagtca aaagatactt ctttggcatg
     5581 cacttttaaa tccttatcta aataaagata aatcggattc attttgttgt ctctcatata
     5641 acatgctaga gaggtgggaa aagaactgtt aaattggttc tttccccaca gatcgctctc
     5701 tttcctgttg gtgtttttta aaccataaag agattctatt ttgctcactt tttgctcctg
     5761 acgtcaattt attgacgtct atggtgtgtt tctatatcat gatgtggagt gatatgataa
     5821 tgagtttttt atgttatgag aataattgat ctttttagtg ggtgtggggg gttgtcccta
     5881 ggctttttga agggggggtt tgatgttgta ggtgcttatg atttctggga tcctgctatt
     5941 gaatgttaca gagataattt tagccatccg ataaaaaaac tagatttgag caatgttgat
     6001 gatgtggtga gggagcttaa agatatagac ttcgatatga ttattggagg gcctccttgc
     6061 caagacttca gtcatgcagg tttaagaatt gaaggtgcta gagctaactt aactcgctca
     6121 ttttcagaaa ttataaaaag aataaagcca aagtggtttg tgatggaaaa tgtagatcgg
     6181 gcattaagaa gtggtgccta tttagaagct cgaggaatct ttaaagaatc agggtatggg
     6241 ttaacagaaa ttgttttgga tgccagtaag tgtggtgttc cgcaaaaaag aaaaagatta
     6301 tttgtaattg gaaagcttga cgtgagggat ggttttatcc ttaacgaagt gatgtgcggg
     6361 atatcgaaag atagtatgac agttagaaat tatcttggtg attcattggg gattgagtat
     6421 tattacagac atccgagaaa ttataacaga agggctattt tctctattga tgaacctgcg
     6481 ccaacagtaa gaggggttaa tagaccgata cctgatggat atttagggca tgcaggcgat
     6541 cctgtcagta tttctgagaa tgtaaggcca ttaacaacct ttgaaagagc aagattgcaa
     6601 acatttcctg aagactttaa atttaaaggt gcaaaaacaa atttggagca aatgataggt
     6661 aatgctgttc cggttgagtt agcaaaatat gttgcggtga caatcatgga gtatg
//
LOCUS       DAZFKY010000226         6709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFKY010000226 DAZFKY010000000
VERSION     DAZFKY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..197)
                     /locus_tag="ACHKCL_004280"
     CDS             complement(<1..197)
                     /locus_tag="ACHKCL_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765121.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYN"
     gene            275..1369
                     /gene="ddlA"
                     /locus_tag="ACHKCL_004281"
     CDS             275..1369
                     /gene="ddlA"
                     /locus_tag="ACHKCL_004281"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5765122.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1393..1605)
                     /gene="yaiZ"
                     /locus_tag="ACHKCL_004282"
     CDS             complement(1393..1605)
                     /gene="yaiZ"
                     /locus_tag="ACHKCL_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5765123.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1865..2173
                     /gene="yaiY"
                     /locus_tag="ACHKCL_004283"
     CDS             1865..2173
                     /gene="yaiY"
                     /locus_tag="ACHKCL_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5765124.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2232..3326)
                     /gene="yaiW"
                     /locus_tag="ACHKCL_004284"
     CDS             complement(2232..3326)
                     /gene="yaiW"
                     /locus_tag="ACHKCL_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5765125.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3339..4559)
                     /gene="sbmA"
                     /locus_tag="ACHKCL_004285"
     CDS             complement(3339..4559)
                     /gene="sbmA"
                     /locus_tag="ACHKCL_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5765126.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4911..6068
                     /gene="ampH"
                     /locus_tag="ACHKCL_004286"
     CDS             4911..6068
                     /gene="ampH"
                     /locus_tag="ACHKCL_004286"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5765127.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6069..6692)
                     /gene="iprA"
                     /locus_tag="ACHKCL_004287"
     CDS             complement(6069..6692)
                     /gene="iprA"
                     /locus_tag="ACHKCL_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5765128.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1707 a         1712 c         1611 g         1679 t
ORIGIN      
        1 ttattataaa taaaagttat gaaatatatt ctcttcgtta acttataatt aagagaaaaa
       61 aactcaccgc atggccattt aagccaggaa taaattagct tcccgttcat catttttgtg
      121 atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca cgctttgtca
      181 gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt
      241 atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt
      301 ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga
      361 tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg
      421 gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt
      481 gcgcccttcg gcgaccagcc tcgcccaggt gccaggtaaa catgagcatc agcttatcga
      541 cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac
      601 gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg
      661 ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg
      721 cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat
      781 cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca
      841 gggctcttct gttggtgtca gcaaagtaac cagtgaagaa caatacgcaa ttgccgtcga
      901 tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat
      961 cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact
     1021 caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt
     1081 agttccggca gccattgcgc cagaaatcaa cgataagatc cgggcgattg ccgttcaggc
     1141 ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa
     1201 cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc
     1261 gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt tgattgagct
     1321 ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc
     1381 ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc
     1441 cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt
     1501 tgcttcaaat cccagtaacc ccaccacgcc attaagaatg aatataaggc caatggcgaa
     1561 cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggctg ctccgttaac
     1621 gaaaaacaac atccttgcat attctgcaac aaccgtctgt ggtgtagaca acattttcgc
     1681 aattgttaat aaatttaact taaagttgct attttgtgac tcgcaacgaa gattcacgct
     1741 tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg
     1801 tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt
     1861 ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc
     1921 ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc
     1981 gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca
     2041 ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat
     2101 cccgttttta gtaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca
     2161 tcgccgccag taagatttac gttgccagat acggcctaaa acgccttatc tggcatgaca
     2221 ttcctgtttg ttcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac
     2281 caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt
     2341 tcgcggggga gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc
     2401 gccgtctcct caaaagagaa gctgtcacct ttctctagct gacgacgaat ttcgctgtcg
     2461 ctcatcccca gttttgccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta
     2521 ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc cttactgacc
     2581 gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaaacgg
     2641 tatatcggtg cgctataact ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac
     2701 ccgccgcggc gactaaagac ttcctgacgg actgtaccgt ccattttcca cggataccct
     2761 ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcaccgga
     2821 ttaagcgaac caaacaacgt ctgccccatt ggcaccatgc tgatcagatc gtcaaaaatc
     2881 ccgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt
     2941 ggcgatttga tttttagcgc agtatgaacc agaaaagcag gaatgtgcat ccgttcagca
     3001 cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg atctgcctga
     3061 taatttgact cctgctgcgc caccgccagc accgagcaga cgttttccag cgtcggtgcc
     3121 agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg
     3181 ctcgcaggca tcttctgccg caccacgcta gccacatcta tcgctttttc gccctttttc
     3241 aacggctgtg gcgcctgact gctacacccc gctaaaacca gaaccgccaa aaatgaaagc
     3301 gaagacagat gattgactcg cgacattact tctccttttt agctcaaggt atgggttact
     3361 tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt gtagatagac
     3421 atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga
     3481 ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac
     3541 ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgagcgatg
     3601 ttgaaataca tatagtgaaa atagaggcgg aaatagtttt tccgtacggc gctaaacagc
     3661 tcgcgtaccg ttggcggcgt cgcgcgcgtg gcatcgtctt caccataaac cagctcttta
     3721 cggtaggcag cctctacacg ctggttttta aactccagcc ccggcagttt gatccctacc
     3781 actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac
     3841 ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac caacaccggc
     3901 aggaaggcga tcaacgtcat gatggcgttg ataaaactga cacccatatt ctccagcgtt
     3961 gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg cgccttcgat atgacgcagt
     4021 tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga
     4081 ctgacaaaga agttattcag cacactgatc actaccgcga tcagcgcaat ccccagaaag
     4141 acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt
     4201 tgaatcagat catagaacgg tgcataccag gcgttgacgg caaccccaac ttccaccaaa
     4261 aaccaggtga cgaagatgat cagtgcagta ccgagtattg accagtattg ccaacggtgc
     4321 gggctgtaga taaaccagaa caatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa
     4381 aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg
     4441 ccggtgatac gcgccaccca gtccccccca ccggcttgcc agaagataac ggcaatcaat
     4501 gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata
     4561 tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct
     4621 acaaagcgtc agccaaatga aaccgcccat tgtgactcat agcaaggcgc ttagttcgcc
     4681 agcctggcca taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg
     4741 gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta
     4801 cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg
     4861 agtagtataa atacgctcag ttaccttcat tcaatttatg gacaccaccg ttgaaacgta
     4921 gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc
     4981 cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt
     5041 acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc
     5101 gcagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc
     5161 gtattgcttc cctcaccaag ctaatgacca gtgagatgct ggtgaaattg ctcgaccagg
     5221 ggaccgtgaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccaa
     5281 cctacaatgg gacgcccatt acgctggtca atctggcaac ccataccagc gccctgcccc
     5341 gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac
     5401 gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag gcggcgtact
     5461 ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata
     5521 cccagttgtt tgaagagcag attacccgtc cattggggat gaaagacacc acctacactc
     5581 cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata
     5641 acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct
     5701 ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc
     5761 agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca
     5821 aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgggga
     5881 ttattcagaa gacaggtggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga
     5941 agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga
     6001 gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg
     6061 catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt
     6121 tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg
     6181 cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag
     6241 tcaatcattg ataataatgt tgcgcggatt tgttcgtagg aattatgccc aatgagctgc
     6301 acgtcgcgta attccagaat tctattttgc caggctaacc agtaaaaagc gtctcgccag
     6361 agttgatttt gttcaataag cgtaatggtt tgtttggctg gtagatgata tcccgtacaa
     6421 tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc
     6481 ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata
     6541 actccctcta aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc
     6601 ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca
     6661 aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcag
//
LOCUS       DAZFKY010000227         6675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFKY010000227 DAZFKY010000000
VERSION     DAZFKY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(25..2808)
                     /locus_tag="ACHKCL_004288"
     CDS             complement(25..2808)
                     /locus_tag="ACHKCL_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5765129.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2865..5237)
                     /locus_tag="ACHKCL_004289"
     CDS             complement(2865..5237)
                     /locus_tag="ACHKCL_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5765130.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5275..>6675)
                     /locus_tag="ACHKCL_004290"
     CDS             complement(5275..>6675)
                     /locus_tag="ACHKCL_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5765131.1"
                     /translation="IKLLTIRWREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQR
                     IAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYT
                     VVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQ
                     ELSDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMK
                     SRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVAD
                     ASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPAD
                     SWYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDIL
                     SSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQP
                     GVWNLADDICDISAVL"
BASE COUNT         1695 a         1539 c         1518 g         1923 t
ORIGIN      
        1 aacgatgtgt tgacggggct gttattattt tggcaataat actccggtat aagtatttac
       61 cggatgagaa agatattgtt taacggcagt gttaacattc tctaccgtca tttgtttcaa
      121 caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac tatttacaat
      181 agtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct gaacgttttg
      241 ctggtattcg ttcagttctt gctcactgat ccctttagtc aaacgcttaa ccatcacttc
      301 attcgctaac gttaacagtt catcatgtcg ttctggttga caagtaaaag ccagcaaatg
      361 actgatatct ttagcctgag gatcaaccga gaggcgagaa gaaacgctgt atgccccaga
      421 tgcctgttca cgaatattaa cgcgtagatc ttttgccagt gcgacgttaa aagcatcgag
      481 cgccatacgc gtcgccagat taacaggtgt ccgggaatca taacgcttcc actgtgaaac
      541 ctgtgccaca ggttcatttt gttcctttac agtaaccgat gcgttgtccg tcgcgcgagt
      601 taatggttta cctgcggcta atggcgaatc agagtgtttg attgatccta agtaacgcgt
      661 aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg tgatatccgc
      721 tggagatgaa aacaactggc gatcggcagc cagcgcatct gcggcagtaa actgtgcaat
      781 ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat acatctgctg
      841 ggcgaatttc tccgctggac gctggtcgag cgttttcaat gcctgaattt gagcattttg
      901 tagcgatgcc caaatattgt cgttaatcgt gctgtgggtg attcgctggt taatcaactg
      961 aaaaccaggt tcagggttat tagtccgcgc gctaacggag agcaacgtat tcatgccact
     1021 gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc tggaagagga
     1081 gagttcgcca acgccgcttc cgctaactgc tttatttgcc agcgcgataa gtgatttttg
     1141 ctgcgcaggg aaacttaaat cgcctttatt cgatacggca ataatttgta gcttttgctc
     1201 ttcacccgcg gattttgcca gaataaccct tgcaccattg gaaagtgtta atgatgtcag
     1261 attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt ctactgttaa
     1321 cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt actccttttc
     1381 cagcgccagg atagccgcag gagacaatgc ttttttggcg gcaacctcat tgtttaccat
     1441 ttgctcccaa aatgcgtcct ggttctttct taactgctgc catttttccg ccagactttg
     1501 cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg gcgacaagaa
     1561 cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta aatcacgttc
     1621 agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga catcatcgag
     1681 ttcttcagca gaaaaaccat gctgatcaat ggttgccaac tcttccatta atgcattcgc
     1741 agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact gataatcggg
     1801 tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc ccgactgtat
     1861 acgttcctgc agacgttgat tgaacagctg aactaacatg ctccattcag cttgttcgat
     1921 aaagctttgc tcatcgttca cttgtaccat tggcaggcga taatagagtg cgatgccgtt
     1981 cacccggttt tctttatcat taatgatatt aaagcgcagg tggttttcgg cttttgtcgg
     2041 ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattat cctttatcag
     2101 cgccagcgct tctttactgt cgatatcgcc aaccacgata aaggtcatat tatttggttg
     2161 ataccagcgt tgataaaatt ggagcaattg tgccggtgtg accgtggcga cagtatccat
     2221 caggccgata ggttcacggt ctaaattacg ggtatttgcc agcaggaaag ggcggcgcgc
     2281 ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa ttacgccgcg
     2341 ttcagcgtct acttcgagtt tttcaaaggt tgcggcatta ctccattcac tgaagattgc
     2401 catcacttgt tgcagatttt gtttctgcgt agttggcaaa ctcacctgat acaccgtttc
     2461 gtcatagctg gtataggcat taacatcgcg accaaaacgc aggcccattg actcaaatgt
     2521 ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat gttctacaaa
     2581 atgagccacg ccgcgctcat tgtcttcttc ctgcaatgaa ccggtatgaa tttgcagcca
     2641 taaatttacc tgatcctttg gatgagcatg cggataaatc atatatcgca agccattgtc
     2701 cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca gccgcccggg
     2761 gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa ttatctccat
     2821 gcgaaaaccg ggcgaattta cccggttaag taaaatccga actattaaaa tttcatgctg
     2881 acatcaagcc agaaagtacg gccactggcg taggtcgcca caccggtatt cgttgtatca
     2941 accgcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag gttttgttgt
     3001 ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct atccagtttt
     3061 tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt tgtcttaccc
     3121 aggataatgc gagctttacg cgcttcttgc caggccagcg tattcgccca caccaacccg
     3181 ctcggttgat gcgtgaaatc catgtttaag gagatcttta atgggttatt aaaatctgcc
     3241 actggaacgc tatcgtaaga gacaagatta ccgttataaa ccacctggtt atcaccggta
     3301 ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat atagctaaag
     3361 acaatttgcg ggttaatatc tacctggctg atatgcaggg gttcggccag ttcaaaactg
     3421 aggttgaacg aatgcgtttt ggttttgccg tcgttgttat attcagtaat ggtggtttta
     3481 gtcgcgctgt cggtacgact gcttttgctg atttgatcat gcgcttcacg gtaaacatag
     3541 tttgcgcgcg caataacgtt cttaccgatt ttttgctgca atcccattgc cagttcatcg
     3601 ttataaggcg ttttcaaatc ctgataacgc gtcagggttt tattacctga taccgattcc
     3661 gtccagctat tgcggatatc acgtaatccc atatcaagaa tattcccgcc atagtaacgg
     3721 ttataacctg ctgtaatcat tgaggtttga tcagcaaaaa tatcccattc cgtcataaag
     3781 cgcggggaga tattgtggtt tgacagatag ttgtcatagt cataccgcac gccgggcatt
     3841 aatgacacat tacgccagct aatgcgatcc gccatataca gtgtgtagtt gtcaattccc
     3901 aggctgcctt tacctttatg gtaaatggta tggttagttt tctttccggc agcattaatc
     3961 acataggatt cagactggtt atggcgttca gtccacgcat cggaatagat gtattccgcg
     4021 ccgaagtagg gttgatgcga aacattaccc acggcgaatt tttgccagtc caggcgtgtt
     4081 ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg ggtgcaacgc
     4141 cctgtaatat caccatatgt acatgaaagt tcggtgtacc agatgtcatg atcgtgacgg
     4201 gtataatcac taatatgatc ccaaccaacg gtggtacgta gtttggccca tgcgagctgc
     4261 gtatccatat cccatgccat accataggat ttattaccca tttcgcgatc agactgcggg
     4321 aaggtgctgg tattataatc acggctggag ccggtatatt ttaaggttaa atcgtgggta
     4381 aagcggtcgc tggcaaacca ggtaaatttg ctcaatgcag tatcgataac gtttttatac
     4441 tgtgcccgac cggcgacaat gccgtcattc gaaacataat ccgcgcgggt gatatcagac
     4501 tggcggcgcg ataaaccggc ggtaacgcca aagttatcag cgagttcctg attaaacgac
     4561 aaggtataaa agttcttttt aaaatctggg gagtaataag tacttcctga agagccttga
     4621 ttaaatgcgc tcttgttatt ctcattgata tgcgatgtta accagtccga acgcgtagtg
     4681 cgataaccca gtttcacgct gctatcatca gcgttgaagc gtttgaactt tgcatcaatt
     4741 accccgccat tgaagcgacc gaattcaacc ggcacaaaac tgtcataaag cgtcacattg
     4801 tccagtaagc tgacatcaag ataatacccc tgtgacatcc cgctaatatt ggttgcacta
     4861 ctggcatcgg actcattcgc tgggttcagg ttattagtgg cactaatacc gtcaatcaaa
     4921 taggcattct gatagggcga cgcaccgtga atagagattt tctccgggcg aatatctccc
     4981 tggttcaacg aggtactttg cgttgaatcc atgcgtaccg cagggttggt tctcagcaga
     5041 tcgctgatat tgccattccc ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg
     5101 ttgccgttta cgggtacagg gacaggagca tagaccgtca tctcttcaaa aaaatgcatg
     5161 tacatgtttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat gacgccagga
     5221 ataagaactc gcttcattta tttatatctc cgtacagcag ggcttttatt gtttttataa
     5281 aaccgcgcta atgtcacaaa tatcatcggc caggttccag acaccgggtt gatgtgtaac
     5341 cataatgacg ccgcttgtgg gtagtttttc acgcactaga cgcagtaagc ggatagcctc
     5401 ttgttcctca agatgagagg tagtttcgtc aagaaatatc catttcggac gtcgtaaaat
     5461 taatcgtgcc agggcgatac gttgtttttc gccgctggaa agaatatctc cccagcgatc
     5521 gtggtcatga atacgcgcag ccaatttccc aagaccaacc tgatgcagta cttcgctcaa
     5581 tgatttatcg tctacgggca ggggaagtgc tttacaaata atctctttca gtaagccggt
     5641 tttgattaac ggtgtttgtg acacatacca actgtcggca ggagaagaaa tatcaccttt
     5701 aaaccacggc cagcagtggg ataatgtttt aagcagtgtg gtttttcccg caccagagta
     5761 acctttcagt aatagccatt tgcctggcga aacatgaaag ttcaggttct ctaatatgat
     5821 cttattatca ggagtacgaa tactcgcgtc agccacttgt accgcatgtt ggcaattttt
     5881 aggcttattc gtagggcgct gttcggtgag ttgatggaac tcatacaagc gatcgataac
     5941 cgcagccagt tcagcaagtt catcatattt ataaataaac cagcttagat tgttcgatac
     6001 cagcataaat gcctggcgcg atttcatcag tccgcccaga ttgatctgcc cgctaataaa
     6061 ctgcggcaat aacagaaagt agggaagaac gctaagcgag cgcgaataaa tattctgcca
     6121 gtaatcaagc caccgctgac gattcattaa acgatgccag ttctctttaa tggtatgaaa
     6181 attgtcgctc aactcctggc gttgtagaga ctcagcattg cttagcgcaa tcagttctgc
     6241 ctgcttatta tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt gtttttccac
     6301 attaagcgga cgaatacgtt tacccacctt atgggtaaat aaagttccac cgatcacaat
     6361 gagcacaacg gtatagacca tatacccctg gatattccat tctgttccac caacagtgaa
     6421 tgagagcata cccgcacttt gccaaagaat aacggtaaag gtgatcagca tgctaagtga
     6481 ctggatgaag ccaaatgaaa gactgagcgt tttgctgatc agtaagagaa tgtcttcagc
     6541 gatacgttgg tcagggttat ctgtattttt atgctcgccg tagatctgcg tgaagtagta
     6601 gtttttatct gcaaaccagc gattaaggta gtaatctgtt aaccattcgc gccagcgaat
     6661 agtcagtagt ttaat
//
LOCUS       DAZFKY010000228         6602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFKY010000228 DAZFKY010000000
VERSION     DAZFKY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(359..631)
                     /locus_tag="ACHKCL_004291"
     CDS             complement(359..631)
                     /locus_tag="ACHKCL_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5765132.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(637..1188)
                     /locus_tag="ACHKCL_004292"
     CDS             complement(637..1188)
                     /locus_tag="ACHKCL_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5765133.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1185..1937)
                     /locus_tag="ACHKCL_004293"
     CDS             complement(1185..1937)
                     /locus_tag="ACHKCL_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO5765134.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2871..3131
                     /locus_tag="ACHKCL_004294"
     CDS             2871..3131
                     /locus_tag="ACHKCL_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765135.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3128..3685
                     /locus_tag="ACHKCL_004295"
     CDS             3128..3685
                     /locus_tag="ACHKCL_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5765136.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3682..3903
                     /locus_tag="ACHKCL_004296"
     CDS             3682..3903
                     /locus_tag="ACHKCL_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765137.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3903..4226
                     /locus_tag="ACHKCL_004297"
     CDS             3903..4226
                     /locus_tag="ACHKCL_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO5765138.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4240..6573
                     /locus_tag="ACHKCL_004298"
     CDS             4240..6573
                     /locus_tag="ACHKCL_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5765139.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
BASE COUNT         1532 a         1614 c         1694 g         1762 t
ORIGIN      
        1 cgagcgctca gttcatttct aaataatttg caaaattatt ttgggtactt tcgcctctac
       61 acttccttcg ggtggttcca caataatgga tctcaccggc gtttgatatt ttataatcta
      121 accccttagc gcgcgcagcc ccccacgcct gctcgcttcg cttaacagac tggttttcat
      181 gcattccgca aatcgtctca gaagccacca cacaagggct ttcgcgtcaa aaatggtgca
      241 tgagaatcat gcgttttcat gcgctataga catgcactca tgcgctctca ggccagccag
      301 ggaaaaggcg taaaaaatcc cagtactgga ccgggattac gtgggctttt tttgctaatc
      361 agacaggaat ttgctgacgg cttgacagct ttgacgcgga gccatagcga ttgagagttt
      421 gctgttgttt ttccggcatt tgtgctttct ctgattcctg ttcattgcgc cggggcttca
      481 tgattatcgt atccacactt tccagtgcgg taaatgtgta ggaacattca agattctggc
      541 actgatacca tgagcgttta accgatggtg cttcataggc ggaggttctg gcgtgtgccg
      601 tcgtaccgca ttcgggacat ttaagtgcca ttaatattac tcctgcatgc cgttaatgta
      661 atccagacgt tgctgaaagc gcagttgttg tgcaggggtg taagagctct ggcaatcacg
      721 aaccatcatg gggtcaggca tcacaccgga ttcatccaga atcatacgat aatccccggc
      781 aatttcaggc ggtgttattg ccagctggcg aaccagagca ttgcgtaata tacgggacgc
      841 aacgtcaacc cctccgcgcc ccttcaggaa cggagccagt gcagatgtca gggctgctcc
      901 attcgcctgc ataaaattgc tgagagcgtc ttctgtgcag gcctccatca gttgatgctg
      961 cgagtaaatg cattcgcgcg cagcacagtt aatttgccat ttcagcacat ccagatgttc
     1021 ccgctgtaat tcgagctgtc gagcataatc tgctggtttt tccatggcag tgaggagtga
     1081 ttctatctgt tgttcaacgg cttttcggct ctcactatgt ccagcccaca gaatacgggc
     1141 atttctaaaa ctgataagcg cttggtctgg agtgactcgg gccatcatac tgctttctcc
     1201 ttctcagcac gttcctgacg aaccttcatt ttatgcatag ctgctggaga gagtttatta
     1261 ctgaaatcca gacgggccgc ataatccggg gcaactcccg ccagtttaaa cacggggtcc
     1321 tgttcgggca tcgcatcgtt agtcagagcc ggtctggtga tatgtttatg aataaaatct
     1381 ttcagcagtg tgtccgggtc attaactgaa tgcaccacac cgacaactgc gctggcttcc
     1441 ctgcccattg ttgatttgag caggcttagt gtctggatta gtgcttttcc atgcgactgc
     1501 ataaaatctt cccagatttg ttttgcgcga atacccacaa gggtgtcgtg cgcgtggata
     1561 tactttccgg ctaattccgc agcctcctgc ggcaaaagtg cattttcgct ttcctgagcc
     1621 gccagcagct catcaaaatc ctcaagcgtt tctcgtccca gcgcaatttc cgtgcgcagt
     1681 tttttcattt cctttgagac tacgccctga ctttcacgaa atagcgtgcg ccactcctca
     1741 ttcagggcgt tcgtcgtggc ttccatttca gcgcggcgct ggcggattgt cgcaatatta
     1801 tccgctgccg ctttttgctg acgacgtgct tcaagccatg cagatttggt ggcattcact
     1861 ttctcaagcg cctgttgcgt cgaaacaggg atagaaattt caacgatcgg gttattgttc
     1921 tgttctggga ttgtcatgtc agcacctagt ggtttgtcgt ggtgtcaatt gtgctgtgat
     1981 gcatacaaag ctgccattga acgccattgt gcgagcgaca agacaaagac tgaaccgccc
     2041 cggacctctt ttggctagcc agaaaaggcc tcgtttaact aaggctgttt cagacttacc
     2101 ttaactattc actactattc acttaggtaa aaaaaatagg taatacagaa ggttataagg
     2161 tgaagggttg gcatggcagt attcatcgac tgttcataac tgttcacatg ggaataaaat
     2221 tatttatggt attttttcat aatgggtggt ttttttattc ctcgtagcta tttgtttttt
     2281 gctcctcacc actattcacc cttattcagg agtattcaac aggcacataa atagactgca
     2341 tgtttttgcc ggggaataac attttcgcct ctggccatgg tgttgcattt catactgatt
     2401 tttataaaac tagatctgtc cggttcttta tggattcatt cggatatatt ttacgattta
     2461 aggtgatgtt atttaatatc aaggttttta tgcaggccca atgtttataa gttggatgcg
     2521 attcaaaaat atgcccggaa attggggcct gcaatgcaag gcctcgtgta gaagttgtac
     2581 tgccttgctg tattggcggt ggggttagca ctatcaggtg caatgaccca taggatagta
     2641 aaccctgggt gaatggtagt tcctattaat gctcctgtat ttatagtccg caggcatggt
     2701 atttattatg tcattttata tggtttaatc gggctttaaa ttccataaaa tcaatatgtg
     2761 acgctaaata aaaaatgcgt gcgcaggtgg tttatataca cataataagg aactacctga
     2821 acccggataa attcatccgg actgttgtgg aaattaaaga gggtagataa atgcgtaata
     2881 tttctgtgtc tgcgccagct cctgctgcgc cattatttcc tgtatcgaac catcatgagc
     2941 gatttttacg cctgcctgag gtgatgcact tatgcgggtt gtcccgttcg accgtttacg
     3001 acctgatcag ccgcgatgcg tttccgaagc agatcccgct tggcggtaaa aatgtcgcct
     3061 gggcacagtc tgaggtcagc gcatggatgg cggaccgcat cagcgcccgt gggcggggat
     3121 gtgatgcatg atgatacctg catacattaa atacctttat tctggcttgc ttactgtcgt
     3181 catttccagg tatagttttt ccgctgtcgc aaaatcggca gccggaattg gcgttccgta
     3241 taacttattg gcgacaattg acgccccttg cgtctttttt tacgtcgtag ctcaggcaca
     3301 accattttca gggctgtggt gtttatgctt acaccatggt tctatcgaga taatggtagt
     3361 ccgggcgggg cagccttcgg gctggccggt ttccaataag gccggttacg ccaaccccgt
     3421 tcgggctgcc accagtgaaa ttggcgtttc cggtggtagc aataaccgct acttattgga
     3481 ggctgccatc atggctacaa tcctcacccc gtcacacccg caatatgtct ttgtgtttgc
     3541 cgcaatccgc cgtgctgaca ctcacccccg catctgtatg cttcgcactg tctcctgcga
     3601 tgagcgttcc gcgcgccgtt tgctagtccg agattatgta ctctcccttt ctgcacggct
     3661 gcctgccggg gaggtgacgc tatgaaccag tttgagattt cctacgacga tgttgtaagg
     3721 ctgaaacatt tacgcaatgt gggtgagtat gtaactggca tggcagccct gcaggactgt
     3781 tatgaaaagc cagcaggtgc ccaatgcgaa caactggttt ccctcattta tctgatgaca
     3841 gagcaactgg atggagtggt gcaacgctgc catgacgacc tgatgaatgc ggaggtggcc
     3901 tgatgatatg ctgtgaatct cttctggcac ttcgtgccgc tctgtatcgc cgtgccgttg
     3961 cctgtgcctg gctggctcta agcaatcatc aggaacgtta ttccggcctg acgctcgctg
     4021 aacttgaaga tgccatagcc cgcgagctgg aagggttcta tctgcgccag catgggcagc
     4081 aaagagggct ggaaattgcc tgcgcgttgc tttcggatct gatggagtcg gggccgctta
     4141 aagcctgtcc ggttctctca ctgctcggaa tgacggtcat ggatgaactt tgttcccgtc
     4201 acctcaacaa accagcgctg cactaaggag ggccgcacaa tgtcaggaat gaaagttagc
     4261 caggctgaga aagcagctcg gggtcactgg tcaagaattt tacctgcgct gggcgtaaat
     4321 gtactgaaaa atcggcacca gccctgcccg gtctgtgccg ggaaagaccg ctttcgattt
     4381 gatgaccagg aagggcgggg aacgtggttc tgtaaccagt gcggggcagg tgatggcctg
     4441 gcgcttgtaa gtaaagtact ggatgtaggc attagtgaag cggcagacag aataaacggc
     4501 attatcggaa acctgctgcc agtatctcag ggaatgcttg aatctggttc tcctgaaaaa
     4561 gaggacggga gaaaagctgc agcagtgctg gctgcccgtt tgtttgataa gtcccgccag
     4621 accactggca atgcctatct gacgagtaaa gggtttcctg cactgccttg ccgggaatta
     4681 accgctatgc ataaagtcgg tggtgtggca tttcgcgcgg gagatcttgt cgttccattg
     4741 tatgcagatg gagagctggt aaatctgcag ttaatcaacg ctaatggggg caaatgcttc
     4801 cttaaaggcg gtcaggttaa gaatgccttt tacctggttg aaggtactgc caaagcagcc
     4861 aaacggctct ggatagcgga aggatatgcc accgcactta ctatcaacta tctgactggc
     4921 gatgctgtca tggtggcctt ttcgtccgtc aatttccttt ccctggcgag cattgcctgc
     4981 agtgagtacc caacgcacca gataattatt gctgctgacc gcgatctcaa cggtgcgggg
     5041 caaacaaggg gcgcagctgt taccggggcc tgcaattgca caatggcgct cccgcctgtg
     5101 tttggtgact ggaacgatgc attcacgcaa aacggcgaag aagccacccg gcatgcaatt
     5161 catgaagtaa taaaaccagc tgttgccagc cccttcgaca caatgagcga agctgaattt
     5221 accgcgctga gcgtcagcga aaaagcgcag agggtagtgg atcactataa aaattcactg
     5281 gcagtagacc cgaacgggca gctcctttca cgctatgagg cgggggcctg gaaagttatc
     5341 tattacgccg attttgcccg tgatgtcgct gcgctgtttc agcgcctcga cgcacctttt
     5401 tcatccgcga aaattgcgtc tctcgtggaa accctcaaac tgatcgttcc gcaacagcag
     5461 aatccggcgc ggcaacttat cggatttcgc aacggtgtgc tcgatacccg gacaggattg
     5521 ttcagcccgc acgataagaa gcactggtta cgtacgctgt gcgaggtgga ttacacgcag
     5581 cccgttgacg gtgagtcact ggaaacccat gccccggcat tctggcgctg gctggatcgt
     5641 gccgcaggtt ttaatcctga aaaacgggac attattctgg ctgcattgtt tatggtgctg
     5701 gctaaccgtt atgactggca gctgtttctg gaggtcactg gccctggcgg aagtggaaag
     5761 agtattcttg ctgaaatagc aaccatgctg gcgggtgaag ataacgcgac ctcggcaacc
     5821 attgaaatgc ttgagtcgcc aagagaacga gctgcgttaa taggtttttc actgattcga
     5881 cttcccgacc aggaaaagtg gagcggtgac ggggccggac taaaagccat cactggcggc
     5941 gatgcggtat ccgttgatcc caaatatcag aacgcctatt caacccacat cccggcggtc
     6001 atcctggctg tgaacaataa tccgatgcgc ttcactgatc gtagtggtgg agtttcacgc
     6061 cgaagggtga tcctgcattt ccccgaccag atagccccgg aggaacgcga tacccagctc
     6121 aaagagaaaa ttgccagtga gctagcggtg attgttcgcc agcttatgca gcgtttcagc
     6181 gacccaatga gtgccaggac attgcttcag tcacagcaga actccgatga agcgctcacc
     6241 atcaaacgtg atgctgattc agcttttgat ttttgcggct accttgaggt cctacctgac
     6301 accacgggca tgtttatggg gaacgctaat attgttccac gtcagcctcg aacttacctc
     6361 taccatgcct atctggtcta catggaggct aacggctata aaaatacgct cagtctgacc
     6421 atgtttggca aggggctacc gttaatgctc aaggaatatg ggctgcagta tgagaaacga
     6481 cggaccaatc aaggaatgca gactaatctg gccctaagag aggaaagcaa tgctgactgg
     6541 ttgccaaaat gcgatgagtt tgcagcgaaa taactgacct aagaccggct gtgccggtct
     6601 ta
//
LOCUS       DAZFKY010000229         6597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFKY010000229 DAZFKY010000000
VERSION     DAZFKY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            140..382
                     /gene="yjbE"
                     /locus_tag="ACHKCL_004299"
     CDS             140..382
                     /gene="yjbE"
                     /locus_tag="ACHKCL_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5765140.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            496..1134
                     /locus_tag="ACHKCL_004300"
     CDS             496..1134
                     /locus_tag="ACHKCL_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5765141.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1131..1868
                     /locus_tag="ACHKCL_004301"
     CDS             1131..1868
                     /locus_tag="ACHKCL_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5765142.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLGGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1868..3964
                     /locus_tag="ACHKCL_004302"
     CDS             1868..3964
                     /locus_tag="ACHKCL_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5765143.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4011..4289)
                     /locus_tag="ACHKCL_004303"
     CDS             complement(4011..4289)
                     /locus_tag="ACHKCL_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765144.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4290..4433
                     /locus_tag="ACHKCL_004304"
     CDS             4290..4433
                     /locus_tag="ACHKCL_004304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765145.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4559..4969
                     /gene="psiE"
                     /locus_tag="ACHKCL_004305"
     CDS             4559..4969
                     /gene="psiE"
                     /locus_tag="ACHKCL_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5765146.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHKCL_004306"
     CDS             complement(5013..6488)
                     /gene="xylE"
                     /locus_tag="ACHKCL_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO5765147.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1618 a         1678 c         1783 g         1518 t
ORIGIN      
        1 tttgatattc attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc
       61 atgctttagt tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat
      121 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
      181 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc
      241 aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
      301 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
      361 cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga gtcattgcat
      421 ctgtggctag aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc
      481 ctctggagaa gagtcgtgaa gcgacctgca ttcattctta tctgcctgct attacaggcc
      541 tgttcagcca cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca
      601 ggcgtacagc tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag
      661 cttaatggtg ggccgcagtt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa
      721 tgggtcactc aggatcaggc cactctcgtc acacaacatg gtcgtctggt gaagactttg
      781 cttggcggcg acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc
      841 gcacaaatca ttgatggcgc aacctggatg cgcacgatgg gctggaccga gtaccagcag
      901 gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg gcaccggtac cgttaaggtc
      961 ggcagcgatg aaaccgcggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc
     1021 tggcataacc gctattggat cgacagcgga gggcaaattc gccagtcgga acagtatctc
     1081 ggcgcggatt atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac
     1141 aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg
     1201 tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa
     1261 tcgatctggt aggccaaccg cggctggcaa acagctggtg gcctggtgcg gtgattagcg
     1321 aagagctggc aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag
     1381 aacagggcgc agattccagc gccgacgatg ccgctgcgat taacgcctta cgccagcaaa
     1441 ttcaggcgtt gaaggtgacg ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg
     1501 ttgctgaacg cgctaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccac
     1561 cgtccacggt cacgttgttc gggcttatca gccgtcctgg caatcagcca ttcactcccg
     1621 gtcgcgacgt ggcgagctat ctctccgggc aaagtctgct tggcggtgcg gatcgtagct
     1681 acgcgtgggt ggtttacccg gacggacgca cacaaaaagc gccggtggct tactggaaca
     1741 agcgtcacgt agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct
     1801 ggagtgagat gcctgatgcc cttaacgccg acattcttca gactctgacg cagcggatac
     1861 ctcaataatg aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg
     1921 ctacggcgaa acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg
     1981 attattacaa acgcccacgg ctcgcatggc gcgggaaggg gagttgagtc tgaactatcg
     2041 cgataacgat cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac
     2101 aacgctgcgc tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg
     2161 cgatcaaacg tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta
     2221 ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc
     2281 ggaatatctt gttgccagca aagcctgggg gccgttcgat tttacgcttg gcctgggctg
     2341 gggatatttg ggcacaagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata
     2401 ttgctatcgc gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca
     2461 cggtcctgcc tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct
     2521 gaaactggag tatgaaggca ataattatca gcaggatttt gccggtaagc tggagcaaaa
     2581 aagtaagttt aacgtcgggg cgatttatcg cgttaccgat tgggccgacg ttaaccttag
     2641 ctatgaacgt gccaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct
     2701 gcgcccgtct tacaacgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat
     2761 tttgcagcat tcggtggtgg cgaatcagtt aacgctgttg aaatacaatg ccggacttgc
     2821 cgatccgcag atccaggcga aaggcgatac gctgtatgtt accggcgagc aggtgaaata
     2881 tcgtgattcg cgcgaaggga tcgttcgtgc taatcggatc gtgatgaatg atctgccgga
     2941 tgggatcaag acgatccgcg ttacggaaaa tcgccttaac atgccgcagg tgacgacgga
     3001 aacggatgtc gccagcctga aaaatcatct cgccggagag ccgttgggcc acgaaacgaa
     3061 gctggcgcaa aaacgcgtcg agccagtggt tccgaagtcc accgagcagg gctggtatat
     3121 cgacaaatcg cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc
     3181 ggaaaacttt tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga
     3241 ccatctgctg accaccggca gcctgtttgc aaatcttgcc aacaactacg acaagtttaa
     3301 ctacaccaat ccgccgcagg actcgcactt accgcgcgtg cgtacccatg tgcgcgagta
     3361 tgtgcagaac gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa
     3421 cggcttctac ggtcaggtct acggaggtta tctcgaaacc atgtttggcg gtgcgggggc
     3481 agaagtgttg tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt
     3541 taaacagcgc gactggcgta gtgcaaagga tatgatgaaa ttcaccgact acagcgtgaa
     3601 aaccggacat ctgaccgcct actggacgcc atctttcgct caggatgtgt tagttaaagc
     3661 cagcgtcggg cagtatctgg caggggataa aggcggcacg ctggagatcg ctaaacgctt
     3721 tgacagcggc gtggtggtgg gcggctatgc cacgatcacc aacgtttcga aagaggagta
     3781 cggcgaaggg gacttcacca aaggcgtgta tgtctctgta ccgttggatc tcttctcgtc
     3841 tggcccgaca cgcagccgtg cggcgattgg ctggacgccg ctgacgcgtg acggtggtca
     3901 gcaacttggg cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg
     3961 ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc acctttattc ttaatggcct
     4021 gttgagccat caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa
     4081 cattctgtct tcctgtccgt cgatatcggt tgcaggtctg gttgcctgag cgactgggcc
     4141 atcattgggc tggagacatt gctttgcggt aggtcggctt tatcagcagt accgagcgga
     4201 ccagcataag caggaagagc agagactgat aacatcaaag cagcaaaata aggcttcatt
     4261 tttaccacct ttatcaggtt acgtttcatt tgttccagag gaacattgtc gatttttcgc
     4321 gcattgctgg tggctgggaa tcacctgaat gggtgatttt tgaattaccg gctttggtgc
     4381 ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc ggcctacggg taggtatatc
     4441 cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa aacatacaca aaaaatatag
     4501 atctccgtca catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat
     4561 gacgtcactc tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa
     4621 tcttggcctg ctgtgcctcg gccttatttt ggttgtcttc ctcggcaaag aaacggtgca
     4681 tctggctgat gtgctgttcg cgccagaaca agccagcaaa tatgagctgg tagaaggact
     4741 ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg
     4801 ttttcacttc ccgctacgtt actttgtcta tattgggatc accgccattg tgcgcttgat
     4861 catcgtcgat cataaatcgc cactggatgt gttgatctac tcggctgcga tcctgttact
     4921 ggtggtcacc ctgtggctgt gtaattcgaa acggctgaag cgggagtaaa aagacagcac
     4981 gccgaactgg cgcggcgtgc tggacaggaa gattacagcg tagcagtttg ttgtgttttc
     5041 ttcgtttccg gttcccagag ggcttccagc tcctcaaggg ttttaccttt ggtttccggg
     5101 acaaatttcc acataaacag tgctgccaga acgcccatac aaccgtaaat ccagtaggag
     5161 aaaccgttgt ggaaatgggc caccagccag gagtttttgt ccatcatcgg gaaggtccag
     5221 gagacgaagt agttcgccag ccactgggcc gccaccgcga ttgccagcgc tttacctcga
     5281 atagcattcg ggaagatttc cgacagcagt acccagcata ccggacccca ggacatggca
     5341 aaggcggcaa catagaacag catcgacagt agcgccacaa tacccggtgc ctgagtgtaa
     5401 aacgcggtac cgaggctaaa cataccgatt gccattccga gtgcgccgat aatttgcagt
     5461 ggcttacgac caaatttatc caccgtcata attgccagaa cggtgaaggt gaggttgata
     5521 actccgacaa taatggtctg caacagcgcg atatccgtgc tggcccccag cgttttgaac
     5581 acttccggcg cgtagtacag caccacattg atgccgacaa attgctggaa gatggagagc
     5641 ataacgccga tgacaatcac gcccacgcca aacatcagca gacgaccacc ggttttgcgg
     5701 ccatgatcca gggagtgttt aatttcctgt actgcctgag ttgcaagcgt gttgcccata
     5761 attttgcgca ggataccttc cgcctgttct tgcttgccgc gtgacatcag ccagcgagga
     5821 ctttctggca cggtatacag cagcattaag aacagcagtg cagggataca ttccgaggca
     5881 aacatataac gccagccgtc agtattcagc cagctggcat caccggaacg cgcaataaaa
     5941 tagtttacgc agtaaactaa aagctgcccg aaaataatcg caaactggtt aaatgaaacc
     6001 agtttcccgc gaatatgagc aggagcaagt tccgcaatat acattggtga aagcattgag
     6061 gctaaaccaa cgccaatacc gccaataata cgataaataa caaattccgg gacataacct
     6121 gccagataaa caggcactgt gttgtccggg tttatagagg taaaaccaag ttctggccag
     6181 gcagaaccta caccagaaat aaaaaacagg acagcagcaa tcttaagtga atcacgacga
     6241 ccgaagcggt tactgcaata accaccgagg gcaccgccga tgatgcaacc aatcagagcg
     6301 ctggccacgc aaaaccctaa cagggagttg gcagcggatt cacttaagtt ttgtggagca
     6361 acaaagacgg tattgagtga ctcaacagta ccggaaataa cggcggtgtc gtagccaaat
     6421 aataaaccac ctaatgtagc gactaaggta atcgaaaata tataactgga attatactgg
     6481 gtattcattc agacctgcct tagaccattc tgatctttta tcagtgcatt gatgttggaa
     6541 ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg tttacgtaat gtctttt
//
LOCUS       DAZFKY010000230         6542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFKY010000230 DAZFKY010000000
VERSION     DAZFKY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            30..758
                     /gene="gadW"
                     /locus_tag="ACHKCL_004307"
     CDS             30..758
                     /gene="gadW"
                     /locus_tag="ACHKCL_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5765148.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1121..4234)
                     /gene="mdtF"
                     /locus_tag="ACHKCL_004308"
     CDS             complement(1121..4234)
                     /gene="mdtF"
                     /locus_tag="ACHKCL_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5765149.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4259..5368)
                     /gene="mdtE"
                     /locus_tag="ACHKCL_004309"
     CDS             complement(4259..5368)
                     /gene="mdtE"
                     /locus_tag="ACHKCL_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5765150.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5476..5754
                     /locus_tag="ACHKCL_004310"
     CDS             5476..5754
                     /locus_tag="ACHKCL_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765151.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5755..6282)
                     /gene="gadE"
                     /locus_tag="ACHKCL_004311"
     CDS             complement(5755..6282)
                     /gene="gadE"
                     /locus_tag="ACHKCL_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5765152.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1640 a         1597 c         1593 g         1712 t
ORIGIN      
        1 aatcgcagta tgaaatataa gggataatca tgactcatgt ctgctcggtg atcctcattc
       61 gtcgttcatt cgatatttat catgaacagc aaaaaatatc gctgcataac gagagtatcc
      121 tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg ttcaccggat acgcgacgac
      181 tggatatcga tgagctgaca gtttgccatt acttacaaaa tattcgtcag ctaccacgca
      241 atttagggtt acatagcaaa gaccgtctgt taattaacca gtcacccccc atgccgctgg
      301 tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa ttcaccgata ctgagcaata
      361 tgctctacct ttcctgttta tcgatgtttt ctcataagaa agaactgatc cccttacttt
      421 tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct tattagcttt gatatcgcca
      481 aacgttggta tctgcgcgat atcgcggaaa gaatgtatac cagcgagagt ctcatcaaaa
      541 aaaagttgca ggatgaaaac acctgtttca gtaaaatatt actcgcctcc aggatgtcga
      601 tggccagacg attactcgag ttacgtcaaa ttcctctgca tactattgcg gaaaaatgtg
      661 gctatagcag tacgtcgtac tttataaaca catttcgaca atattatggt gtaacgccac
      721 atcagtttgc gcaacattcg ccaggtacct tttcctgaca tattttgcat ttgaatattg
      781 gtcaggatct cacacctgct tcatgtgaaa ctcttccctg atgatttctg ccgggctacc
      841 ggctagttct ctttcgcagt ttgccaggcg gtataggtat tttcaggagg aaaagactcg
      901 gcatgtttgg gattattaag ctgacaattc atacgattac gggaatgtgg gtcagtattg
      961 tgctgttcaa attgatgacg aatggctgga gtggtttcta ctttcagtgc tgtgtactaa
     1021 gcctggtatt cctgacggtt agctggttgt tgagtggtga atggttagca ggaaagagta
     1081 aggctgaacc ttcacgttca accttactct catttacacg ttacgctttt ttaaagcggg
     1141 caaagagatg ttccactaca acgaaaaaga ccggaacaaa gtaaattgcc agcactgttg
     1201 cggcaaacat cccgcccatc acgccggtac ctaccgcgtt ttgcgcgcca gaaccggcac
     1261 catgactgat aaccagcggc agcacgccga gaataaaggc cagagaggtc atcaggattg
     1321 ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat cggcgttttc ccttctttct
     1381 gcatcatctc aacggcaaat tcgacaatca ggatggcgtt cttggcggaa agcccgatgg
     1441 tggtcagcaa accaacctgg aagtagacgt cattacttaa gccgcgcaga tcggtggcca
     1501 gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac cgagaacgga attgaccagc
     1561 tctcataaag tgcggcgagg gcgaggaaca ccacgaccag tgaaatcgca tacagcgcag
     1621 gagcctgatt tgaggataac gcttcctgat acgacagtcc ggtccatgag tagccgacgc
     1681 ctgccggaag tttagcgacc aggtctgcca taaatttcat ggcgtcaccg gtacttttcc
     1741 cggccgccgc ttcacctaaa atctccattg acgggatgcc gttgtagcgt tccagtcgcg
     1801 gtgaaccata ggtccattcg gtagacgagt aggcagaaag cggtgccatc gtgccagagg
     1861 cgttgcgtac ataccattgg ttgatgttat ccggcaacat acggaacggc gtgcctgcct
     1921 ggacatacac ttttttcacc cgcccctggt tgaggaagtc gttcacgtag ctgctgccga
     1981 aggcggtgga aattgtctgg ttgatatcag acagcgccac acccattgcc tcagctttcg
     2041 cggcgttgac gttcactttg aacatcggcg tatcttccag gccgttcggg cgtaccccgg
     2101 tgacctgatt cggtgattgc gctgccagtg ataacagctc gtttcgtgcc tgggttagtt
     2161 tttcgtgccc caggttaccg ttgtccagca gttccatatc aaaacctgac gcggtaccca
     2221 gttcagccac cgcgggtaag ttgaacggga agacgacggc tttattgata ctgcttagcg
     2281 caatcattgc ccgctgaatg atcgcggtaa ccgagttttc ctcaccgaca cgttcagacc
     2341 acggcttgag actgataaac gccagaccgt tgttttgccc ctggccgctg aagccaaagc
     2401 cgccaacggt aaacaccgac tgaacattat ctttctcttt tgtaagataa taatccgtca
     2461 cctgttgcag cactttcgtg gtgttaacca tggtggcacc ggaaggtaac tgcgcggtgg
     2521 tcataaatac cccctgatcc tcttctggta agaaagaggt cggcgtgcgc aggaacagca
     2581 ccgccatccc ggcgcaaatc agcaggtaga tcaccatgta gcgaccggta caacgcaaca
     2641 gtgagcgggt gctatcggta tagtgctgag ttgatttttc aaacagcgtg ttgaagcgtg
     2701 cgaacagggc attaggtttg tgaccgcctt ccggcgcggc tttcagaatg gtggcgcaca
     2761 gggcaggggt caggctcatt gccacaaata ctgaaagcag catggaggag atcagcgtga
     2821 tggagaactg gcggtagatc tccccggttg caccgctcat aaaggccatc ggcataaaca
     2881 ctgcggaaag aacaacggca ataccgacca gcgcacgttg gatctgcccc atcgatttat
     2941 gcgtcgcttc cttcggtggt agcttatctt ccgcaatgac acgctcgacg ttctccacca
     3001 ccacgatggc gtcatccacc agtaacccta tcgccagcac catcccgaac atagtcaacg
     3061 tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt cccgagaata accaccggta
     3121 cggcaatcgt cgggatgatt gtggcacgga aattctgcaa aaacagatac atgaccagga
     3181 agacgaggat gatagcctca accagtgttt tgaaaacttc ctgaatagaa atttcgataa
     3241 acggcgtggt gtcgtaagga taaaccgtct tcagacttgc cgggaaatag gctgataagc
     3301 ggttcagttc ctctttgact gcccgcgagg tatccagggc gtttgctccg gtagccagtt
     3361 tgatggcgat cccggcagca ggtttgccgt tatagcgcgc cacggtggaa taatcttccg
     3421 ccccaagttc gacgcgggcg acatcacgca gcagcacttg cgaaccatct tgctgaactt
     3481 tcaacaggat tttgccaaat tcttccggcg tttgcagacg cgtctgcaca atgatcgagg
     3541 cgtttagttg ctggtctgcc gcctgtggca tgccacccag ttgaccacca gaaatctggt
     3601 tgttttgcac cttaatctgg gaaataacat cggaaggtac cagattgtat ttattgagtt
     3661 tttgcgggtc cagccagata cgcatggcgt actcggaacc aaagagctgt acgctaccaa
     3721 cgcccgcggt acggcttagc gggtctttga tattagacgc tacatagtcc gcgatatcgt
     3781 actggttgag gctgccgtta tcagaaataa acgccgctac catcaggata ttactgctcg
     3841 atttatcgac gctaatcccc tgctgctgca ctgcttcagg taatgaaggc atagcgagtt
     3901 gcagtttatt ttgcacctga acctgtgcga tatcaggaga tgtcccagtc tcgaaggtca
     3961 gagtgataga ggcattaccc gccgcatcac tggttgaaga catgtacatc aggccatcaa
     4021 gcccattcat attttgctca atcacctgag tgaccgagtc ttctaccgtt tgcgcatcgg
     4081 cacccggata ggtagcgctg acggtaatgg tcggtggcgc aatctgcgga tactgcgcaa
     4141 ccggtaagtt catgatcgcc agaccacctg caagcatcat aataatggca agtacccagg
     4201 caaaaaccgg gcgatcaata aaatagttag ccatgaaagt ccccttaagc ctgcaacgtt
     4261 attgtttcga ttcggtgctg gcgttttcct ggctggagga aattgctcgt gctttgatac
     4321 ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc gccagcctgc aagccagagg
     4381 tgacaaccca ctggtcgccg atggctttgc tggcttcaat ttcgcgtagc ttcacgacat
     4441 cgtctttatc cagaatgagc gccgttgctt taccttgggc gttgtgggtg acgccttcct
     4501 gcggcaccag taatacattc tggcggctac cttcatccac taatgccgtg acatacatgc
     4561 caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg gagcccgtgg
     4621 tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtaa cgtttaccat
     4681 tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc ccactggcga
     4741 cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca taaatcgggt
     4801 ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt gcgccgacgg
     4861 tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg tattgcagat
     4921 tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct gcttcattca
     4981 actgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc agcaacgatg
     5041 cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc gccagcgagc
     5101 ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac agcgaatcgc
     5161 cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg cccacctggg
     5221 gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc aacacattga
     5281 ccgacccagg ggagagtgtg acgacaccga cctcaggcgt catggcggcg gtgttttccg
     5341 ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt attaacagct
     5401 ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt ctttaaattg
     5461 cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt gtcagccagt
     5521 aaggtgggat acaggcatag tgatcgacat ggtgaggtca acgacaaaaa aaatgttgcc
     5581 gttctgccaa cagttcctgc cagcattcgg gcaacgaagg gttctactgg tggatacaca
     5641 taccagggga ataaagtcgt tttagcctct cctttagtaa tcaccggggg taatgaaatc
     5701 agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc ctgactaaaa
     5761 ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca gcttgtacat
     5821 aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata ttgagcgtcg acgtgatatt
     5881 gctttcattt ttatttttca gaatcagttg cataattttg aactgattat gtgaaaaaca
     5941 taattgtgaa ttcttatggg gcaagtgttt accataagta atctcaagaa gatttttgat
     6001 actttctttg cggctaacaa aataaatagg cgctttagct tttagtttac tgatgtgata
     6061 aggtgccaaa acaataattc tctcggcatc taatttctcc agaaatttaa tcgcttcttc
     6121 atcaaggata tgattgtgat aggtatcaat gataaccttg aagggtttat tgcctacttt
     6181 tttgatctct gacagggaat tgatttttat catttcgtga ttatctttca actgccaaaa
     6241 gccctgtaaa agaaaagaat ctttcgtcat gagaaaaatc ataacttgct ccttagccgt
     6301 tatcgtttcg aacatgtcat ccttttcatt atttacatcc ttgtccgaat cgttgttcaa
     6361 tatagtaaac gcctataaga actttgccct tcgtgaacta atacgtctat taacactaat
     6421 caatacttca gatttatcca tcacgcactc catatattga tcctaagtaa gattcctggt
     6481 tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact gcccaagaat
     6541 aa
//
LOCUS       DAZFKY010000231         6415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFKY010000231 DAZFKY010000000
VERSION     DAZFKY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(163..2196)
                     /gene="betT"
                     /locus_tag="ACHKCL_004312"
     CDS             complement(163..2196)
                     /gene="betT"
                     /locus_tag="ACHKCL_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5765153.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2325..2912
                     /gene="betI"
                     /locus_tag="ACHKCL_004313"
     CDS             2325..2912
                     /gene="betI"
                     /locus_tag="ACHKCL_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5765154.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2926..4398
                     /gene="betB"
                     /locus_tag="ACHKCL_004314"
     CDS             2926..4398
                     /gene="betB"
                     /locus_tag="ACHKCL_004314"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5765155.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4412..6100
                     /gene="betA"
                     /locus_tag="ACHKCL_004315"
     CDS             4412..6100
                     /gene="betA"
                     /locus_tag="ACHKCL_004315"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5765156.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
     gene            complement(6102..>6415)
                     /locus_tag="ACHKCL_004316"
     CDS             complement(6102..>6415)
                     /locus_tag="ACHKCL_004316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765157.1"
                     /translation="SSTLFWSDLFLSSLQEPFSYMLTVSGSEQVTIARNTSGVILKLS
                     GISPAVYQCIHSTFISEIERVSLEFAGEVVTMKHESYPYVLYNDSVPIPGTVTLNLIG
                     S"
BASE COUNT         1576 a         1777 c         1764 g         1298 t
ORIGIN      
        1 aaaataaaat cgttaattaa taaaatataa aatcatgaag tgatacaaga ggaatttcag
       61 gatatgacat gacaatcata tataatatat gcataatttt ttacagcgag tcaatgattc
      121 ccgcgccata tgcagacatg gcgcggtttt atgcaataac aatcacgcgt ccgggaacat
      181 cacgctatgg cccggcgctt cacgatggag atgaataaag ttaaggtgcc gctcgtactg
      241 gtcaagaata tcggtgatca cctgctcttt gctgtagtcc atcaggtcgt tgccctggct
      301 gccttctaac aggaaggttt ccagccggta gtaggtcgat ttcccgctac gtgcgcggta
      361 ggtaaagccc ggcaccgagt attgctgcgg ccaaatctga tagacaaagt tttgctcttc
      421 gcccatatgc accaacaaat ccagatgccc caactgttgt ccctcttccg gcggcaggct
      481 ttttagctcc acgtacgcgc cgcgcaaccg caactcctgc gccacttctt ccattgccgg
      541 gtaacagacc gtctccatca tctgtttagt gtaacgcgtg cccggataat tcatcaggcg
      601 cgagagacgt tttttccagc tcaggcgatc ctgaagcccc agcggtcgcg gtgcggtatc
      661 gcggttggca ctttcacggc ggtaatcttc taccttcaga gatttataca accccgccat
      721 cacgaagaag atcacaaagc tgaacggcag ccccataatc accgtggtgt tttgcagcgc
      781 ggatatcccg ttggtcatca gcatgccgag cgtcagcagg ccaatcgcca ccgaccagaa
      841 gacgcgcagc cagccggggg cgtcgctgtt gatatcttta agctgcgagg tgaaattccc
      901 cagcaccagc gccccggagt ccgccgaggt cacataaaac agcaggccag taatggtggc
      961 gacggaggcg ctaaaggtaa acgccggata ctgcgccagc aggctgtaga agccgcgctc
     1021 cggatggacc atcgcttcct cggcaaatgc cgcgccgccg tggatgattt catacagcgc
     1081 gctattgccg aacaccgaga gccataacag cgtgaaggta aacggaataa tcaacgtgcc
     1141 cagcacgaac tggcgaatgg tacgcccacg cgagatacgc gccaggaaca agccgacaaa
     1201 cggcgaccat gccacccacc atgcccagaa gaagagcgtc cagttattca tccactcaac
     1261 cgggcggtcg aaggcaaaac tgttgagcgt catgcccata aagcgattca catagtcgcc
     1321 aacattcagc accagtgcat taagcaggaa cgaagtgtcg cccataaaca acacgaacag
     1381 gatcaatccc agcgccagcg cgacattaag ctccgataac acgcgaatgc ccttatcgac
     1441 accggatgtc accgagatag tggcgattat caccgacaag gcgatcagtg ccgcttttgc
     1501 cgccatcgaa tcgggaatat caaacagtac gctcaagcca tagttaagct gcaccacacc
     1561 gataccgagc gtagtggcaa taccgaagat agtgccgatc accgctgcaa tatccactga
     1621 gtgacctatc ggcccgttaa tccgtttacc gaagatcggg tacagcgccg agcggatggt
     1681 gagcggcaaa ttataacgat agctaaagta tccgagcgcc atgcccatca gcgcatacat
     1741 cgaccagccg gttaagccgt agtgaaacag cgtccagacc atcgcctgac gcgcggcctc
     1801 aatcgtctgt cccgcgcctt ccggcggctg catatactgc gttaccggtt cggctacgga
     1861 aaagaacatc agatcgatac cgatcccggc agcaaacagc atcgccgccc aactcagcag
     1921 gctgaattcc ggtttggatt gttctggccc gagcttcacc gaaccaaaac gcgaacaagc
     1981 gatacagacc acaaagacaa tatagagcgt tgccgccagc agatagtacc aaccgaaggt
     2041 tttagaaacc cagtccagcg tgcggccaat ccacagggcc gagaagtcgc gaaacaggat
     2101 cgttgtcagg gaaaacaaca aaatcagtcc ggcggaggtg taaaacacca ccggattgat
     2161 tttgtccttt tccctgctgt gtgaaaggtc tgtcatccag tatccccact gttattgtta
     2221 ccatttaaaa tcaaattcgt aacaattaag acacatttta tattgaacgt ccaatcaata
     2281 accgctttaa tagataaaca acgctgatga atggagtggc aaaaatgccc aaattgggga
     2341 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2401 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2461 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2521 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2581 agcgattaca ggcgattgtt ggcggaaact tcgatgaaac gcaggtgagc agtgcggcga
     2641 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2701 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2761 cgcgccacca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2821 tgcgcgcggc actgagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2881 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2941 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     3001 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgcgaggat
     3061 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     3121 atggaacgct cgcgtattct gcgtcgggcc gtcgatattc tgcgcgagcg caatgatgaa
     3181 ctcgccaaac tggaaaccct cgacaccgga aaagcgtatt cggaaacctc aaccgtcgat
     3241 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccgtc gctggaaggc
     3301 agccagatcc cgttgcgtga gacatcattt gtctacactc gccgcgaacc gctgggcgtg
     3361 gtggcaggga ttggcgcatg gaactacccg atccagattg ccctgtggaa atccgccccg
     3421 gcgctggcgg cgggtaatgc gatgattttc aaaccgagcg aagtcactcc gcttaccgcg
     3481 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggtgtatt taacgtgttg
     3541 ccgggcgtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3601 tcgtttaccg gcggcgtcgc cagcggcaaa aaagtgatgg ctaactcggc agcctcttcc
     3661 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgacgatgcg
     3721 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3781 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca acgccgcatt tgagcagaaa
     3841 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3901 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3961 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cctcgataac
     4021 ggcgcatggg ttgcaccgac cgtgttcacc gattgcagcg acgagatgac catcgtgcgt
     4081 gaagagatct tcgggccggt gatgtccatt ctgacctacg agtcggaaga cgaagtcatt
     4141 cgccgcgcca atgataccga ctacggcctg gcggcgggta tcgtgacggc ggacctgaac
     4201 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4261 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggtattgg ccgcgagaac
     4321 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4381 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4441 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgtt
     4501 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgctgc
     4561 cctggcattc ccgctacagg gtaaacgcta caactgggct tacgagacgg aacctgaacc
     4621 gtttatgaat aaccgtcgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4681 gatcaacggc atgtgctata tccgtggcaa cgcgatggat ctcgacaact gggcaaaaga
     4741 acccggtctg gagaactgga gttatctcga ttgcctgccc tactaccgca aggccgagac
     4801 ccgcgacgtg ggcgagaacg actaccacgg cggcgacggc ccggtgagcg tcaccacctc
     4861 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4921 cccgcgcacg gacgatctca acggttatca gcaggaaggt ttcggcccga tggatcgcac
     4981 cgtcacgccg cagggccgcc gcgccagcac cgcgcgcggt tatctcgatc aggccaaatc
     5041 gcgcccaaac ctaaccattc gtactcacgc catgaccgat cacattattt ttgactgtaa
     5101 acgcgcggtg ggcgtcgagt ggctggaagg cgacagcacc attccgaccc gcgcgacggc
     5161 gaacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5221 tggcgtcggc aacgctgaac tgttggccga gtttgatatt ccgctggtgc atgatttacc
     5281 cggcgtcggt gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5341 accggtttcc ctctaccctg ccctgcagtg gtggaatcag ccgaaaatcg gtgcggagtg
     5401 gctgtttggc ggcaccggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5461 cagccgagag gaatttgcgt ggccgaatat tcagtatcac ttcctgccgg tagcgattaa
     5521 ctataacggc tcgaatgcag tgaaagagca cggcttccag tgccacgtcg gctcgatgcg
     5581 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5641 tctgtttaac tacatgtcgc acgaacagga ctggcaggaa ttccgcgacg caattcgcat
     5701 cacccgcgag atcatgcatc aacccgcgct ggatcagtat cgtggccgcg aaatcagccc
     5761 cggcacggaa tgtcagacgg atgagcagct cgatgagttt gtgcgtaacc acgccgaaac
     5821 cgccttccat ccgtgcggta cctgcaaaat gggctacgac gagatgtccg tggttgacgg
     5881 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5941 gattatcacc gggaatttga acgccacgac gattatgatt ggcgagaaaa tggcggatat
     6001 gattcgcggg aaggaagcgt tgccgaggag cacggcggga tattttgtgg caaatgggat
     6061 gccagtaaga gcgaaaaaaa tgagtcgtga tttgaactga ctcaggaacc gatcagattg
     6121 agagttaccg ttccaggtat ggggacagaa tcgttatata aaacataggg gtaactctca
     6181 tgcttcatag tgacaacctc gccagcaaat tctaagctga ctcgttcaat ctcgctgatg
     6241 aaggtactat gaatgcactg ataaaccgca ggtgaaatgc cactcagctt aagaattacg
     6301 ccgctagtgt tcctggctat ggtgacttgt tcactcccgc tcacggttag catatacgaa
     6361 aagggttctt gtaacgagga caaaaataag tcagaccaaa acaacgtgga gcttt
//
LOCUS       DAZFKY010000232         6391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFKY010000232 DAZFKY010000000
VERSION     DAZFKY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..354
                     /gene="ydjO"
                     /locus_tag="ACHKCL_004317"
                     /pseudo
     CDS             1..354
                     /gene="ydjO"
                     /locus_tag="ACHKCL_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(358..1749)
                     /gene="tcyP"
                     /locus_tag="ACHKCL_004318"
     CDS             complement(358..1749)
                     /gene="tcyP"
                     /locus_tag="ACHKCL_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5765158.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESIYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1882..2472)
                     /gene="ydjM"
                     /locus_tag="ACHKCL_004319"
     CDS             complement(1882..2472)
                     /gene="ydjM"
                     /locus_tag="ACHKCL_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5765159.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2635..3303)
                     /gene="hxpB"
                     /locus_tag="ACHKCL_004320"
     CDS             complement(2635..3303)
                     /gene="hxpB"
                     /locus_tag="ACHKCL_004320"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5765160.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3450..3986
                     /gene="yniB"
                     /locus_tag="ACHKCL_004321"
     CDS             3450..3986
                     /gene="yniB"
                     /locus_tag="ACHKCL_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5765161.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4027..4887)
                     /gene="yniA"
                     /locus_tag="ACHKCL_004322"
     CDS             complement(4027..4887)
                     /gene="yniA"
                     /locus_tag="ACHKCL_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5765162.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4993..5283)
                     /gene="ghoS"
                     /locus_tag="ACHKCL_004323"
     CDS             complement(4993..5283)
                     /gene="ghoS"
                     /locus_tag="ACHKCL_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5765163.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5384..6313)
                     /gene="pfkB"
                     /locus_tag="ACHKCL_004324"
     CDS             complement(5384..6313)
                     /gene="pfkB"
                     /locus_tag="ACHKCL_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5765164.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1653 a         1589 c         1569 g         1580 t
ORIGIN      
        1 tgtcgggagc atttttgctg cgattcaaaa aaatgagagt ctcctaaagt ttccttcgat
       61 aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg gaatgggaag
      121 tggtatactt gaagatatta tgagtattcc agatagttca cttagaaaac gtctcgggta
      181 tgaggtttta agcttctcgc tacaggcaca ttcattgagc caggaatgta ttgataagtt
      241 ggacattttt tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa
      301 catttaaaaa gtaaggcaac ggccccttta caaaacggac cgttgccagc ataagaatta
      361 atggtgtgcc agttcggcgt cgtcttcgct atccagaatg gctttatcgg tttgcttcag
      421 ccactggctg gtcagcgtgc cagctgtcat cgagccacta acgtttagcg ccgtacggcc
      481 catgtcgata agcggttcaa cggagattaa cagcgccacc agggttactg gcaggcccat
      541 cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg caacgcctgc
      601 ggagctaacg gtaacaatac cgaccagcgt cgcaatccac atcgggtcca gcgggttaat
      661 gccaaccgta ggcgcaacca tcaccgccag cattgccgga tacaaaccgg cgcagccgtt
      721 ctgaccaatg gtcgcaccga aagaggcggc gaaactggcg atggattcag gaacaccgag
      781 tcgacgcgtt tgtgcttcca cattcagtgg gatagacgca gcactggaac ggctggtaaa
      841 ggcaaacgtc agcacaggcc ataccttacg gaagtacttc agcggactca cgccattaat
      901 gcccagcaga atgccatgca ctgcaaacat aatcagcaga ccgaggtagg acgcgacaac
      961 gaaacttccc agtttgatga tgtcttgcag gttagaacct gcaaccactt tggtcattag
     1021 tgccagaacg ccgtaagggg tcaactgcat gaccaggcga actagtttca tcacccagct
     1081 ttgcagggta tcgatagcgg ttaagacgcg ttcacctttc ggcgcatcat ccttcagcag
     1141 tttcagcgca gctacaccga ggaatgcggc aaaaattacc acgctgataa ttgacgtcgg
     1201 attggctccg gtcagatcgg caaacgggtt tttcgggata aaggacaaga ccagctgcgg
     1261 aacgctcagg tcagagactt taccaacata gatactttcg atggcgttca gacgtgcagt
     1321 ttctgcacca ccctgaacca gaccttcagc cgtcaaacca aacaggttgg ttaccagcac
     1381 accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccaatggtca gaaaactgat
     1441 tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag aggcgaagac
     1501 taacggcata acgatcattt gcagcagttg aacatagccg ttaccaacaa tgttaaacca
     1561 ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca gggcaaggcc
     1621 aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc actgtttatg
     1681 gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg caattaatgg
     1741 aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca gtcatagtgt
     1801 tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat tgcttattac
     1861 agaaaggcga tttttgcctt tttagtattc aacctgatgc ttaataagcc gatgaaactg
     1921 gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgtttt cgggtaatga
     1981 atgaggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt ccagttggtt
     2041 acccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc agagcagggg
     2101 aacaccggcg ggtgtcagca tatcggcaag tatgtggctt aaataaccca gcaccattcc
     2161 ttgtagcgca tcagccggaa tgaaccagct ttcaggaacc ttaaggtaaa aggttgccag
     2221 cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag cgcgggcgat
     2281 cggttttgat atccacttta atcgctgccc aagaaacgac tttgggtgat cgatgtccgg
     2341 taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct gtgccagcac
     2401 gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga gaaggtgacc
     2461 ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg atttttatac
     2521 agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta atccgtcagt
     2581 tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg gtcattaacc
     2641 gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg ctaatacaaa
     2701 acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc gggctgcttt
     2761 agaggcgatc atgccattta ccgaatcctc cagcgctacg caggtcagtg ggtcaacgcc
     2821 cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag gcagtttttc
     2881 ggccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca ctttttccag
     2941 catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt tgcataacgc
     3001 aacggcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg aaatggcacg
     3061 ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt gccgggcgta
     3121 ccaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct cgttacgacg
     3181 ggagatatcc acccccaggc ttgccatcac atccagttcg gctcgatccc ataaaggttc
     3241 tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac gcggggttga
     3301 cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct tatgattaag
     3361 cgcctatcat atcgccttta aagaatgggc gaaaacggat atctacaggt aaactaagtg
     3421 aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga cgcattgctg
     3481 ttttgaaaag gattatgggt tgggtgattt ttatacctgc attgatctct acattgattt
     3541 ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat gcggtcatgc
     3601 tcgactttac tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt ttgaatctgt
     3661 tctggtataa ctccccgacg cccaatttta atggtggcgt gaacgttatg ttctgggtga
     3721 ttttcatcct gatttttgtt ggactggcgt tgcaggattc cggtacccgg atgagtcgcc
     3781 aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa gccaaagggg
     3841 aagaggggct aacgcgtgaa caaattgagt cacgtattgt tgttccgcac catactattt
     3901 tcctgcagtt tttctcactg tatatcctgc cggttatttg tattgctgca ggttatgtgt
     3961 tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca ttatcttcaa
     4021 cccatatcac gcagctaata atctatccag tgactgctga gcaataacca aatgctgacc
     4081 gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt gacgttcaag
     4141 gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg gcggctgttc
     4201 agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc aggccgggtc
     4261 gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat cgccgtgcaa
     4321 cagagaaggc tgcggttgat gtgaggcgag acgctgctga atatgctcga cgatggcatc
     4381 gatattgccg aaagcgatcc ctttttctgc tgccagttcc aactgccagc caatccgttg
     4441 ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag ttgtggagag
     4501 cgagttatca aaatcgagtc caaattgtgg ttggtcgctc cattgatgta aacgcgcaat
     4561 ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga gataatccat
     4621 caccagaaaa ctgtagtcac ggtcagcgcc aactgcccaa accttaggca cggtgacggt
     4681 tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaaccgg gaagcagttc
     4741 cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc atgcggcatg
     4801 gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta actgctcgct
     4861 caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc cagatcataa
     4921 ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga ctgattattg
     4981 cccaaaaacc atttattttc tgttgctatt ccattcctcc caggtcgtga tggtgatttc
     5041 aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa tttcttcttc
     5101 actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat ttccctcatc
     5161 atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc
     5221 agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat cgccactggc
     5281 catagttcac ccctttgtta tcacatgact tttgagcata gtcggagaaa cgcgttgccg
     5341 acaggttggt gatgattccc ccaatgctgg gggaatgttt ttgttagcgg gaaaggtaag
     5401 cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg agtgtggctg
     5461 cactccccgc agctacgcca aaacgaacca tctcttcaag agaggcattt tctgccagtt
     5521 tcagtgtcat cgcgccgacc atgctatcac cagcgccaac ggtactctgg cttttcaccg
     5581 gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct tgtggaccca
     5641 gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg gctttgcgga
     5701 catcgtctgg ctgggtgagt tcgcgattca ccagcgcact gagttctttt tggttaggct
     5761 taaccaactc gatgttacca atcgccagtg ctgcacttaa cgcttcgcca gaactgtcga
     5821 tgatgcagcg gatcccttgg ttttgcgcag cggaaatcag ttgggttaat ttttccagct
     5881 tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca atttccagaa
     5941 cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc ataacaaaac
     6001 gatactgctc accgcttgct tccacatgta cgtgtaaatt ctgccgggtc cagtctttgg
     6061 cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg tgttcgccgg
     6121 tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca atggcgcggg
     6181 cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc agttttcctt
     6241 cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat
     6301 agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga atcagcggaa
     6361 agtgaagaaa ttaacaatat gatttattgg a
//
LOCUS       DAZFKY010000233         6339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFKY010000233 DAZFKY010000000
VERSION     DAZFKY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2399
                     /locus_tag="ACHKCL_004325"
     CDS             <1..2399
                     /locus_tag="ACHKCL_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5765165.1"
                     /translation="TAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKPGIPR
                     PALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDTCENI
                     TVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKW
                     PEIQAEMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREY
                     KQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDA
                     LRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVP
                     ETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAA
                     GATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVKKIMQAANISQPDA
                     EELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQKAEQNSPNTQQNE
                     PETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIFDGENQPEVQENDP
                     EEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRAGIHLMIDLET
                     MGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDTMKWWLKQSREAQS
                     AIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRRSYERQGSPCPWRY
                     YNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADS
                     "
     gene            2472..2723
                     /locus_tag="ACHKCL_004326"
     CDS             2472..2723
                     /locus_tag="ACHKCL_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5765166.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            2758..2945
                     /locus_tag="ACHKCL_004327"
                     /pseudo
     CDS             2758..2945
                     /locus_tag="ACHKCL_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(3417..3503)
                     /locus_tag="ACHKCL_004328"
                     /pseudo
     CDS             complement(3417..3503)
                     /locus_tag="ACHKCL_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3709..4035)
                     /gene="ynfA"
                     /locus_tag="ACHKCL_004329"
     CDS             complement(3709..4035)
                     /gene="ynfA"
                     /locus_tag="ACHKCL_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5765167.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            4170..4511
                     /gene="ynfB"
                     /locus_tag="ACHKCL_004330"
     CDS             4170..4511
                     /gene="ynfB"
                     /locus_tag="ACHKCL_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5765168.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4546..5106
                     /gene="speG"
                     /locus_tag="ACHKCL_004331"
     CDS             4546..5106
                     /gene="speG"
                     /locus_tag="ACHKCL_004331"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5765169.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5109..5819)
                     /gene="ynfC"
                     /locus_tag="ACHKCL_004332"
     CDS             complement(5109..5819)
                     /gene="ynfC"
                     /locus_tag="ACHKCL_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5765170.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            5927..6232
                     /gene="ynfD"
                     /locus_tag="ACHKCL_004333"
     CDS             5927..6232
                     /gene="ynfD"
                     /locus_tag="ACHKCL_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5765171.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1894 a         1508 c         1455 g         1482 t
ORIGIN      
        1 ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac tggcaattcc
       61 tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc gaggataaac
      121 ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag gaaaaccgct
      181 gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atccgatccg atgaacgtca
      241 cttttgacaa cctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg
      301 aaaacatcac tgttgatatg gtgattagcg cacaggaatt gttgcaggaa gacatggcaa
      361 cattcgacgg ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac gctatgtatc
      421 cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat
      481 ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg
      541 aagccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat caacagcaca
      601 ctgaaaattc aacaggaaaa atcaacccgg tcattgctgc cattcatcgc gaatacaagc
      661 agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg
      721 ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacggcc
      781 gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg
      841 accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac aaagaccctg
      901 tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt gaagatgaag
      961 gaacaaatca gagcgcaact gatactctct cgtcgccagt accagaaact gatgcagtgg
     1021 aaacggcaat tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag
     1081 aacgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg
     1141 caaacaaact ttctggtctg gataaggcgc tggctgccgg ggctactgaa atcacaaaag
     1201 aagaatattt tgcccgaaaa aatggcacat acacaggctt accgcaaaat gcaaataccg
     1261 cacaaaattc tgaacaacca gaaccggtaa aagttaccgc tgacgaagta aagaaaatta
     1321 tgcaggcagc caatatcagc cagcctgacg ccgaggaact gcttgcagta tcacgtggtg
     1381 aatttgttga agggattagc gacccgaatg atccgaaatg ggttaagggg atccagaccc
     1441 gcgattctgt gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa gcggaacaaa
     1501 acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgaacca gtagtgcaac
     1561 aggaaccgga aaagatctgc accgcctgcg gtcagagcgg tggcggcaac tgccctgatt
     1621 gtggtgcggt gatgggtgac gcaacatacc aggaaatatt cgatggagag aatcagcctg
     1681 aagttcagga aaatgatccg gaggaaatgg aaggtactgc acatcagcac aaggagaaca
     1741 ctggcggcaa tcagcatcat gccagcgata gtgaaactgg cgaggcgtca gatcccttaa
     1801 ttaaggcgaa cggtcatcat aatctcacat ccaccagcag agcggggatt catctgatga
     1861 tcgatcttga aaccatggga aaaaatcccg atgccccgat tatctcaata ggtgcaatat
     1921 ttttcgatcc gcaaaccgga gatatgggac cggaatttag taagactatc gatctggata
     1981 ctgctggcgg agtcattgat cgggacacca tgaaatggtg gcttaaacaa tcacgcgaag
     2041 cgcaatctgc cattatgacc gatgaaatcc cgttagatga tgcactgtta caattgcggg
     2101 aatttatcga cgaaaactcc ggtgaatttt ttgttcatgt ctggggaaat ggagccaact
     2161 tcgacaacac gattttgcgc cgttcatacg aacggcaggg gagcccctgc ccgtggcgtt
     2221 actacaacga tcgcgatgta cgcacaatcg ttgagctggg gaaagccata gacttcgatg
     2281 ccagaacggc tattccattc gaaggtgagc gccataatgc acttgatgac gcccgttacc
     2341 aggcaaaata cgtttcagct atctggcaaa aactgatccc gagtcaggct gattcttaat
     2401 gttcaactgt cgccggttgt gactggtatt ctgcaaccgg cgctcgtctg atgtaagaga
     2461 taaagaaatc gatgagcgaa gtaatcatga ttgtctctcc cggcaaatgg gtatccgaag
     2521 agcagttaat tgcgctgaaa ggaataaaaa aaggtacgtt aaaaaaggcc cgggaaaaat
     2581 cgtttatgga aggaagggaa tataagcatg tcgctcatga cggtatgcca tgggataaca
     2641 gtccatgctt ttacaacctg gaagaaattg atcgctggat tgagcgccag gcatcagcaa
     2701 gaccaagacg tcatcttact tgactaaaag ccacactaac taatgagaga agttgaaatg
     2761 aaatatccga caggcgtgga aaaccatgga gggaaattac gtatctggtt tgtttataaa
     2821 ggcgtaagag tcagggaaaa cctggggttc ctgacacagc aaaaaacagg cgcgttgcag
     2881 gtgagctacg cgcctctgtt tgttacgcaa taaaaactgg tgttttcgac tatgcaaaac
     2941 agtttccctc ctcacgcaat ctggaaaaat ttggtgaggc ccgacaagat ttaaccataa
     3001 aagaactggc tgaaaaattt ctggcactga aagaaactga agtcgcaaaa acactcaaca
     3061 cataccgtgc cgtcatcaaa aatatcctga gcataatcgg tgaaaaaaat cttgcctcat
     3121 cgattaataa agaaaaatta ctggaggttc gtaaagagct actgactgga taccagatcc
     3181 ccaaaagtaa ctatattgtt acacaaccag ggagatcggc tgtaactgta aataattaca
     3241 tgacaaatct taacgccgtg ttccagtttg gtgttgataa cggttacctg gcagataacc
     3301 cgtttaaggg gatctcgcca ttaaaggaat caagaaccat tccggatcct ctttcgcggg
     3361 aggaattcat ccgtcttatc gatgcgtgca gaaatcagca agccaaaaat ttatggtgta
     3421 ataccgtcct cagtggtgat ttttaatgtg acgaaattac gccccggaca ggtaacaaaa
     3481 acttcagcct ttacgatctt catgttcgat tccttgcatc gcttgtcgtg atgcatgaaa
     3541 tctacgcaac tgagctacta ccatataagt atgaagatcg aaaaaagccg gagtgatcac
     3601 aaaaaaaggc gtatatttgc gctgtgaatg gttgacaaaa gatgaaatag aatacctttt
     3661 gtcagctgac acttcctcgt atcttattga taaaatggat ttatgttcct acgtgcgccc
     3721 ccagcccaca acaatgatca acatgccgca aagcgcaatc aacggccccg tccagtcata
     3781 aagagtcagt ttcacgccat ccacaacgcg cagccacatc aacgccgtgc agacataaac
     3841 gccaccataa gccgcgtaaa cacgcccact cgccgcagga tgcaacgtta acaaccagac
     3901 aaacagcgcc agtgaaatcc ccgccggaag caacagccag atactggcgt ttcgttttaa
     3961 ccacaaccag ggcagaaagc atccaataat ttcacacagc gcagtagcaa aaaatagtaa
     4021 cgttgtttta atcatctttg tcacttattg acatcatgta tagttatagg gcgacataat
     4081 atcatcaata taaacaccct cctggtacgt tttgcgttcg cagtggatgg tgtagaatca
     4141 tctttaatca ttcatacagg gaatgaatta tgaaaatcac tctcagcaaa cgaatcgacc
     4201 tgctcgcttt tctgctgcct tgcgcactgg cattgagcac aactgttcat gccgaaacta
     4261 acaaactggt gattgagtct ggcgacagtg cacaaagccg ccagcacgcc gctatggaaa
     4321 aagagcaatg gaatgacacg ggcaatctgc gccagaaagt gaataaacgc actgaaaaag
     4381 agtgggataa agccgacgcc gcttttgata accgcgataa atgcgagcaa agcgccaaca
     4441 tcaatgccta ctgggagcca aatactttgc gctgcctgga ccgtcgaact ggccgcgtta
     4501 ttacccccta acctgttatt gatttaagga atgtaaggac acgttatgcc aagcgcccac
     4561 agtgttaagc tacgcccgct ggagcgtgaa gatttacgct atgtacatca actcgacaat
     4621 aacgccagtg tgatgcgtta ctggtttgag gaaccctacg aagcctttgt tgaactctct
     4681 gatctgtatg ataagcatat tcacgatcag agcgaacgac gctttgtggt ggaatgtgac
     4741 ggcgaaaaag ccggtctggt ggagttggtg gaaattaacc atgtacatcg ccgcgcagaa
     4801 tttcagataa ttatctcccc ggagtatcag gggaaaggtc tggcaacccg tgccgccaaa
     4861 ttagcaatgg actatggctt caccgttctc aatctctata agctgtatct gatcgttgat
     4921 aaagagaatg aaaaagcgat tcacatttac cgcaagcttg gcttttcggt tgaaggtgaa
     4981 ttgatgcacg agttctttat taatggtcaa tatcgtaatg ccattcgcat gtgtatattc
     5041 cagcatcagt atctggcaga gcacaaaaca ccgggtcaga ctctcctgaa gccgaccgca
     5101 caatagcatt aataataatc gatcgtattt ttgatggtgt aaacccgttc gacggcgggt
     5161 tttactcctt catcaacaat gatcagctga cagcccaccg gattggcgtg actgtcatat
     5221 tcacagctct gttttacatt accaactcgt tgattattca gtaaagtaac cgctgtgtaa
     5281 tctaattttt tgatcgggtc cgttgatggc gtggcgctga cagataatgt tttgtcgtta
     5341 cttttcgtgg ttttacccag tggataaccc tgatcatcat agcgatattc catctgcatt
     5401 tgtttgctgc tggctttaat cacgaagcca ttatcatcgg tttcccaact caccccggca
     5461 gaaggtaatt ctgctagctg gcattttccc tgtaaacgta ctctcttctc cagcgtctcg
     5521 gcatcacggt aataattggc gtccagtacc agagcgacca cggtattatt ttccagatcc
     5581 agtaattcga gtgaatcaaa acagccttct tccgacaaag tccccgaaac acgtttcgtc
     5641 acttcacctt gctcatccat taatgtctga gtgaaatctt ttaccggacc acgcagcgga
     5701 tcaaaatcga attcattaga gaaactggcc atctcaggag taaatgaaag tgttacctct
     5761 gtgcggtcac atcctgtgag taatatcgcg agtaagcatg gcagtaattt gtatttcaca
     5821 tcagtcacca gagagtagag atgattctca atcatagtag caaatacagt actttacacg
     5881 tgaaatgcta tgcttaaaga agttatcttc gcgttaggag cttatgatga aactctcaac
     5941 ctgttatgcc gcgcttctgc tcgccctcgc ctcacccgcg gtactcgccg cgccgggatc
     6001 ctgtgagcgc atacaaagcg atatatcaca gcgcattatc aataatggcg tacccgaaag
     6061 cagcttcacg ttaagtattg tacccaatga ccaagttgat cagcctgatt cccaggtcgt
     6121 cgggcattgc gctaatgata cgcataaaat tctctacacc cgcaccacca gcggtaacgt
     6181 ctctgctccc gcgcagtcta cccaggatgg cgcgcctgcc gaaccgcagt aatacattat
     6241 cgactacacg ccggatacga cacatccggc gatttggaca tacaacataa tcccaccata
     6301 ttactcatac ccttctattg atatggatta ataattatt
//
LOCUS       DAZFKY010000234         6218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFKY010000234 DAZFKY010000000
VERSION     DAZFKY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            177..311
                     /locus_tag="ACHKCL_004334"
     CDS             177..311
                     /locus_tag="ACHKCL_004334"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765172.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            912..1733
                     /locus_tag="ACHKCL_004335"
     CDS             912..1733
                     /locus_tag="ACHKCL_004335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5765173.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1733..2839
                     /locus_tag="ACHKCL_004336"
     CDS             1733..2839
                     /locus_tag="ACHKCL_004336"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5765174.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2929..4650
                     /gene="cptA"
                     /locus_tag="ACHKCL_004337"
     CDS             2929..4650
                     /gene="cptA"
                     /locus_tag="ACHKCL_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5765175.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4724..5722
                     /gene="lpxM"
                     /locus_tag="ACHKCL_004338"
                     /gene_synonym="msbB"
     CDS             4724..5722
                     /gene="lpxM"
                     /locus_tag="ACHKCL_004338"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5765176.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5775..5963
                     /locus_tag="ACHKCL_004339"
                     /pseudo
     CDS             5775..5963
                     /locus_tag="ACHKCL_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6067..6153)
                     /locus_tag="ACHKCL_004340"
                     /pseudo
     CDS             complement(6067..6153)
                     /locus_tag="ACHKCL_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1601 a         1353 c         1552 g         1712 t
ORIGIN      
        1 aataaatgaa caataatttt atgtatttaa aataaattga ttttacttct gtaatggtgt
       61 catgattagc agctttttaa actaatatcg actttcaata aataaggaca ggtaatggac
      121 aacaaaaata tctgaacccg ggtgagattc ataagttgca cagtgctgtc ttcaaagtgc
      181 cacacccgga acgtaatcac tgcctgctcc ttatggggta tcttcatggt gtccaagcat
      241 cagagttatt aggtataaaa ctgtctgata ttgatttgca ggccggtaac ctgaatatcc
      301 ggcggctcta aacggtttgt caactcagcc cctcggctga aacaggaggt acagtccatt
      361 cgtcgctggc tgaaacttcg ggatgaatgg gtagaagagg gatatgaatg gttatttaac
      421 ctctatggca ggtctgctac aggtattact gttgttctgg ggggcactgg tgtggttaat
      481 atctacgatg caagagattc tcacgatggc ccggtccatc agtgcatgcg gcgcgatatt
      541 gtgcgagtat ttttcagggg gagtggtatc cggatgcaga aaattccgct actcgactgg
      601 ttttatgcaa taaatcagga tgataatctt ctgatttttt actgaggcgg atatctctcg
      661 ctgtgtttac tgaaattgcg gtattctgtt ttttctgaaa aacagaaaat ctgattaaaa
      721 agagcatatg cgttaaggga aatttaagtt ctggttgatt aactgataaa aatattaaaa
      781 aatccctgtt gtcggtaatc tccggaccat tccgggatta cacaataata tcaccatgtg
      841 tgatatctat ggatcctcac tgttgtttac gcgaggatat tttcagaatg cttaacagac
      901 gattattttt tatgttgaat gcaaggcatc ttcccgtact gatgtatcat catgtcagtc
      961 gttgtccggg gcttgtgaca ctctcacctg aaacattttg tgaacagatg aagtggctcg
     1021 cagataataa ctggcggacg gtaacatctg ctgagcttga atatttttat caggggggaa
     1081 cgcttccacg aaagagtgtc atgctcacat tcgatgatgg ttatcttgat aactggctca
     1141 gagcatggcc ggtcctgaag gaatataacc ttcatgcaca tatttttctc attacggggc
     1201 tgattggtaa gggaaacgta cgtagcaggc aggagcagga atattctcac cgcgactgtg
     1261 aacggttgat tgccgataac cgttcagatg aggtcatgct tcgctggtct gaagtccggg
     1321 aaatgcgtga ttccggactg gttgaatttc atgtgcacac ccattcacat aaacgctggg
     1381 acaggttgtc tgtatcacgt gctgaacagt gccggctcat gaaagaagat attctggtgg
     1441 gaaaacaatg tctgacggaa aagctggggt tctgtagttc ccatctgtgc tggcctgaag
     1501 gatattataa tcgggattat attaatctcg ccggtaagct tggtttctct tatttatata
     1561 caaccgaaag aagaatgaac tgtcctgaaa atggttcatt acgcataggg cgtatcagca
     1621 ccaaagagcg ggaacattcc ggctggctga aacgacgcct tttttattat acaacgccac
     1681 ttttttcttc agtactggct cttcataagg ggccaagatt accagataat taatgatgaa
     1741 aattttattc actgaatctt cgtcagatat tgggggccag gagctgcagg cactggccca
     1801 gatgactgct cttcaaaagc agggacattc cgttttactt gcctgcagag aaaaaagtaa
     1861 aattgctccg gaagccagaa aaagagggca tgatgtcact tttattcctt tcaggaacag
     1921 ccttcacctt ccttccatcc tcagacttcg acggattatc ggggaattta aacctgatct
     1981 ggtcatctgc catagtggcc atgacagtaa tatagcagga ctgagccgtc tgatatgctg
     2041 tcaccgcttc agtattgtca gacaaaaaac ctacatcacc cgtaagacca gaacgttttc
     2101 cctgaattat ctgtgtgatt tcattgttgt gcctagctca gccatgatgg cgcatctgat
     2161 ggcggaaggt gtcaggacac cggtaacggt tattccaccg ggtttcgact ggcccgcatt
     2221 gcataatgaa gccatgcgac cgttacccct gcatatccac gcctgggctg catcagcaga
     2281 caatgttccg cttatcgttc aggtggggat gctacgtccc gaaaaaggac atgaatttat
     2341 gctgcgtgta ttgtatcagc tgaagatgga agggaaatca tttcgctggc tcgtggtggg
     2401 cgccgggcgt gaagaatacg aagcacgtct gcgtcagcag acagaacacc tgggaatgag
     2461 cggagatgtg ttaatggcag gtgccctttt cccagcgtta ccggtctacc ggattgccag
     2521 tgtggtggtc atgccttcgg aaaatgaagc gtttggtatg gtactggctg aagcgtcagt
     2581 ctccggcgtg cccgttatcg ccagtgagac gggcgggatc cctgatgtca ttcagaagaa
     2641 tgtgacaggg acgctgttac ccgtggggga tgtgtctgca tggaccggcg cactgcgcga
     2701 ttttttatcc cgaccggaac gcttccggat gatggcagcc agcgccagag aagatattga
     2761 gtaccggttt gatattaaca gaacggcaca gatcattgtg tctctggcta gtcaggctaa
     2821 ggggaaatgc aacagataac ggcggatcct cttttcagcc cgaattttgt tcagcggttt
     2881 tccggtaacg gagtaattta tcacattgtt taaatacggg gacggtcaat gcatttaaat
     2941 accggacaga acaggcctac tttcagctgg agcgcgctgg gatgggcaat tttttatttt
     3001 ggcttttttt ccactctcct gcaggtcatc atcttcagca gtgggtacag cggaacgaat
     3061 ggaatacggg actcactgtt attcagttgt ctgtggttga tcccggtgtt tctctatcct
     3121 gatcggataa aaataattgc agctgttgtc ggtttcattc tctggggcac gtcgctggca
     3181 gcactttgtt attattttct ctatggtcat gaattctctc aaagtgttct tttcgttatg
     3241 tttgaaacga atgccagaga ggctggtgaa tatttcagcc agtatttcag ccttaaactg
     3301 ttgcttatct cgctggtata tactgcggtg tccgtttttc tgtggacacg tctgcgtcct
     3361 gtatatattc ctttgccatg gcggagaatt gtctctttcc tgctgcttta tgctctgctt
     3421 ctgcatccgg ttgttctgaa atcgttaatc agacaggagc cgctgaatga tactctaggc
     3481 aaactggcat cccgaatgga gcctgctgcc ccctggcagt ttgtatccag ttattatcag
     3541 taccatcagc aactgaatgc actgacaacc ttcctgaatg aaaatagcgc actgccacca
     3601 ctgggtaatc tcagggatga atcaggggag agaccacgca cactggtcct ggtgattggt
     3661 gagtcgacac agcgcgaacg catgagctta tacgggtatc tacgtgaaac gacgccggag
     3721 ctggatgcac tgcgtaaaac cgatccgggt cttactgtgt ttaataatgt ggtggcatcg
     3781 cgtccgtaca ccattgaagc attgcaacag gcccttactt tcgccaacga aaagaatcct
     3841 gatctgtatc tgacgcagcc gtcgctgatg aacatgatga agcaggcagg ctataaaaca
     3901 ttctggatta ccaaccagca gacaatcaca gcccgtaaca ccatgctcac tgtattttcg
     3961 cgccagacgg acaggcagta ctacatgaat cagcagcgaa cacaaagtgc gcgtgaatat
     4021 gacactaacg tgctgaagcc gttccgggaa gtgctgaatg accctgcacc aaagaaactg
     4081 atcatcgtgc atctgctggg tacgcacatt aagtataaat accgttatcc ggaaggtcag
     4141 gggcggtttg atggcattac agggcatatt cccactggat taaatgcgaa agagctggaa
     4201 gtgtataacg attacgataa tgccaatctg tttaacgatc atgtggtggc cagtctgata
     4261 aaggacttca gagcaactgc gccggacggc tttctgcttt atttttcaga ccatggtgaa
     4321 gaagtatatg atactccgcc atataaaacg cagggacgga atgaagataa ccccacacgt
     4381 cccatgtaca ctgttccgtt cctgctgtgg acctcggaaa agtggcatgc tgcacatccg
     4441 cgagattttt cgcagtatgt tgaccgcaaa tacagtctgg ctgaactgat ccacacctgg
     4501 tcagatttgg cgggactgac atacgatggt tacgatccga cccgttctct ggtgaatccg
     4561 caattcaggg aaaccacccg ctggattgga aatccgtata aaaagaatgg gctcactgat
     4621 ttcgacactc ttccgtatgg tgagccatag tattttctta tcttcggttc cgttctgaag
     4681 gcaggaggat gtgttttcct ctgctgtccg gtggcctcca gggatggtac caccttctgc
     4741 tgtactctgt tatcataatg agataagcag gcagataccg gtaaatatga aaaatatcag
     4801 aactgagttt attccccgct ttaaccttac attatgcttc ccccgttact ggatgacgtg
     4861 gacaggtatc ggtattatct gtgtgtttgc aatggtcccc cccgcactga gagatccatt
     4921 gttgggaaaa ttgggcatgc tggttgggcg tctggggaaa agcgcacgtc aacgggcctt
     4981 gattaattta tcgctttgtt ttccggagta cagtgataaa gaaaaggaga atatcgttga
     5041 tgccatgttt gcaacagcct ccatggccgt tgtgcttatg gctgaattag cactgtccgg
     5101 gcctgataaa atttcgcatc gtatacgctg gaatggtctg gagattgtgg agaagatggc
     5161 acagaacaat gaaaaggtca tttttcttgt gccacatgcc tggggagtgg atattcccgc
     5221 aatgctgatg gctgcgtcgg gcagaaaaat ggcagcgatg tttcataacc agagaaatcc
     5281 ggtggtggat tatgtctgga acagtgttcg tcgtcgtttc ggaggaaaac tgcacgcacg
     5341 taatgacggt attgcctcct ttgtccgctc agtccggcag ggatactggg gatattatct
     5401 tcctgatcag gatcatggcc cggaattcag tgagtttgca gatttttttg cgacgtataa
     5461 agcaacttta ccggttatcg gacggctcag caggatcagt ggtgcacgga taataccgct
     5521 tttccctgtt tatgacggaa agacccatca tctgactatt catgtcagcc cgccacttgc
     5581 tatacgacag aagagtgatg ctcatattgc gcgacagata aacgaagtgg ttgaaaattt
     5641 cgttcgtcca catcctgagc agtatacgtg gatcctcaag ttgctgaaaa cgcgtaaaga
     5701 aggggaagag gatccttact gaggcagctt tttcaggata atgagcttac ccagcaatag
     5761 tggacacacg ctaagtgagt aaactctcag tcagaggtgg ctcatatgac aaaaaaacag
     5821 taccaaccag taaaaaaccc cgtaaatagc attcgcctga atttcgcagt gaagccctga
     5881 agcttgctga acgcatcggt gttgctgtcg cagctcgtgt gctcagtctg tatgaatcac
     5941 agcttgtaag cggctcgtca gaactgtatt gatatttact gagcggtcag ttcaacgtaa
     6001 gcgagtgtta tgcgccatgg aagcttaccc aggaatatgt tatcaggcct cagcatggcg
     6061 aagtgttgca acgctggaag cagaatgacc gcggtcaaca acttgtttta ccgcttcaat
     6121 tttaaaatct tcgggataac gcttaccgct catgggcacc tctttttaag ccatttaaaa
     6181 tgactctgag gtgtctgtta aacccgtggc gattcaat
//
LOCUS       DAZFKY010000235         6190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFKY010000235 DAZFKY010000000
VERSION     DAZFKY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(82..726)
                     /locus_tag="ACHKCL_004341"
     CDS             complement(82..726)
                     /locus_tag="ACHKCL_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765177.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(747..1016)
                     /locus_tag="ACHKCL_004342"
     CDS             complement(747..1016)
                     /locus_tag="ACHKCL_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5765178.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1095..1733)
                     /locus_tag="ACHKCL_004343"
     CDS             complement(1095..1733)
                     /locus_tag="ACHKCL_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO5765179.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(1718..2950)
                     /locus_tag="ACHKCL_004344"
     CDS             complement(1718..2950)
                     /locus_tag="ACHKCL_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5765180.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(3451..5619)
                     /locus_tag="ACHKCL_004345"
     CDS             complement(3451..5619)
                     /locus_tag="ACHKCL_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO5765181.1"
                     /translation="MIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEAFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(5616..6131)
                     /locus_tag="ACHKCL_004346"
     CDS             complement(5616..6131)
                     /locus_tag="ACHKCL_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765182.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKNVIRHGV"
BASE COUNT         1695 a         1555 c         1202 g         1738 t
ORIGIN      
        1 catattttga tgtagtaaca ccattcactt tcatctaccc cccgttttat taacatatag
       61 taaaaatcac ctcgccgcat tttattcttt tgaagtaaaa tcatttacca gttcacttaa
      121 agctctcttt tgttcatcat catacagaga ttgaatccgg aatttaatct cttctgcaac
      181 cagtcttgca tcgggctcat aaaatatttt tgttattcgc tgtttctgat gttcaggctg
      241 gtcttccata tttcgtaaaa ccagatgaaa taatatatca taaagtgcgc aatgccatga
      301 ataaccatca ttggttaaaa taagctccca aacccgctcc tgctcggcaa taagctgcag
      361 aacaaaggac tcaacctgtt ctgtttgttc gggggcaaca cggtatagcg gagcaatcac
      421 agcaaaagat agttcattta accaggattc cgcttccttc tcgctcctga acggtggaca
      481 gttttgtatt cttcgttcgt attcacgggt aatctgctcc agaatatcct ccggcggtgt
      541 cagccactta ttcctgagca atacactggt tgatggatga cagctggcta tttcagcaat
      601 aatttcacgt aatgtctccg ggctgaactt cttcagctga ttgtagtgct cccgggtaaa
      661 accatgttca tcgcataaaa gttgccatgt cattgatata ctgtttgcca ttccttgctt
      721 catcattaat acctgttacc tgaatatcaa cagaccggtg taccttttct gaatacctcc
      781 ccggacggag tggcaatccc cattcgcctc agcgtggcat ctgcagcttt cgccacctcg
      841 ctgatatcca gcgtttcagg tagcggcggg gacgatatct gcgccagaca ataattccgg
      901 cgcaacaaca accactcccg ttgtgcttct gtacattcaa catttcccat aacaaaaagc
      961 cagcggtcag aagctctgcg ccagcaacca gcagcctcca gtttctgcgc aaccatgtct
     1021 tccacaactt ccggggtact ctttttcctg ctcaaaacat taactcactg aaataatata
     1081 catatctgac ggtctcagcg aaccgtccat gccgggctga aatcctcttc ctgaaacagc
     1141 tccgtcagcc cgctaatctg tttcaatctg agaacttcat cctggtccat acccagctcc
     1201 gtaccaatgc gttcattcgt ccagcccagg cgcgacaggt ccctgacaat atcagacatt
     1261 gaggttatct gatgtttgcc cctggcccgg ttatgtcgga tggtcgcagc aatacgtgag
     1321 gcctgtccct ttctctccgg attaatacag accaccggca gccagccctt cagacgtttt
     1381 cccgtacctg cttccctgcc cagcacctgg cgatgaaaac catccaccac caggtaatgg
     1441 ttcttttcct cagacaccac caccggttgt gtgaaaccat ctttctccag tgactgcttc
     1501 agcagtttct tctcacccgg cgccatgaca ttaggattgt aatcattggc caccacctca
     1561 tctgctttta cccacagcac acagtccact ggctcatgtg caaaaggact gacttcatga
     1621 agcactctgc gcaggtgatt aagggcctgt atggtctctt cttctggtcg ttcctggctg
     1681 aagtactgac gaatcatgtt gccaattatc tccggtgtca cagaatcccc cattccttcc
     1741 tcctttcttt catacgctgc agataacgtt catagtgccg gggtttgttc ggactgaagg
     1801 agaggggccg gcaccagaaa tcattcttta taagtgtctt acagatacgc cgccaggacg
     1861 gaatatcccg gctccccagg tcattctcct gttcatccgg gatgtcatcc gggaagcccc
     1921 gcgtctgata ccagcgcagg tagacagcaa ttttattacg gtaatgttct gccgttcttt
     1981 ccggcatcac atccagcagg aacatcgcat agctgcgcca ggtatgatgg gctggttttg
     2041 atatacgttt acgcagggca aaataggcac cgctttcatt ggcataaagc gccccgctgg
     2101 cagcgcctga cacccgctca cacatcctgg cccaggtttc cggctccaga acatggtaaa
     2161 gccacagccc cttacgttgt tcaggaccaa aaggttcaca gacacgcatg ttgcgtaacg
     2221 gcacgtcggc acggtacatc aggtcataca gaggattgta aatggcacaa gctctggcgt
     2281 tatatatcca gatatcgcgg gctttccagt catacaacgg atacatggtg taataaaacc
     2341 cttcaggcga tgccgtggtc cagggcttat catcggcata tctcagtttg cgctgagaca
     2401 ccagccccat aaagcgattg agggattcat ccgcacgaac accggtcagc actgccactc
     2461 cacaccggtt accggcaaac caggaagaaa atgccggaac aaattcttca aacgtcatgg
     2521 cataccggaa aaagggaaaa tatgtcatat ccgtaatggc ctcttccggt ggctggcgaa
     2581 cccaggtcac tcctggctcc cagcatatcc actccggctg aaactgcgag acgccgttta
     2641 ccgtggtcag gggaagtgcc acccagtaaa aggtttccgt cacatcatgg tacatttccc
     2701 gcatcttctg aatatgttca atggtgcacc gatactgggc ctcccagtca atgaacagaa
     2761 cagagaaatg acgttttctc ctgcgggcca cctctgccac aagatggaaa agcacggtgg
     2821 aatcctttcc acctgaaaaa gacagacata ccgacggaaa tgtttcaaaa atccactcaa
     2881 tgcgggcgat tgcagcacgc aaaacatttt ctccggtaag gattttttcg gttaaaagcg
     2941 tatcactcat tttcctggct ttatgtcgaa taattctgtt tatacattca ggcaaacttc
     3001 attaataact caccgctttg gggataaata ataacaacaa aaatcttcag atattaacct
     3061 ggttgttgga tttgttatca catcctttat ccacctgctt cttactggta acacacctgg
     3121 aaaatccatg cattttcaac atatactatc ctttcaactt gtaaataaga aaatatgatg
     3181 cagatggtga acaattcaaa tatcattttg caggaagtat tcactgtaat agaattaccc
     3241 cctgataaag tttattcact aaggaattat tcaacagaaa cattccctgt tagctttgag
     3301 caatagcact tatttttata tgtatcagga atacgtacgt tgatgtaacc aaaacaaaga
     3361 agcctgtgtc catggggttc gtacaattgt gtgtggcaca tgaccaaatc aggtttgaga
     3421 cctcccgctg caatattttt tattcagatt ttagcctgtc aggcctgaca gaaacgctcc
     3481 caacttccct tctatatcac tgtctatagt acgtaatctt agcaatggca ggccgcattt
     3541 cttcagtata ctgtttttaa gttcatcacg ttcagcctga acactggtca gatgataacc
     3601 tccatccact tcaataaccc ctaatggagt cttcccgacc ttatagtaaa caacaaagtc
     3661 acaactggcc cggttacgca tgaataactg ttcacggtgt gtaaaatccc cccgctccag
     3721 caacaccaac tgattcagcg ctatttgtga atgaaacatc atactccgat aactatcctg
     3781 cgacaggata tcccgcaaca ggcaggccac aatctgttct gacttaaagt gtgaatcatt
     3841 tgaattcaat ctactattca gtcgttccag tgatttgtca tactcactgt acagcaaatc
     3901 aaatgctgat atgaccgggc tctcgaaaat ctctccgtca tcagcgtagt atttgatgta
     3961 acgaatcagc gccgcaatat gtccagcgtg gcgttcaaat acatcattac cagtcaccag
     4021 cgtaaattta tttcgggccc ttgaaacagc gacattcacc agttcaggat tatcaacaaa
     4081 cgcgatgttc cgactgtgct ggctgctgcg cttcttatcc agcacagtgg aaaacacaat
     4141 ttcatcgcac tcccgtccct gaaatttatg caccgtcgat ttcacaaaat cagcaggcag
     4201 cactttttca gccagattaa cctgtgcatt atatggggca atgtatcccc tgctactttc
     4261 ttcatcccag taatgacctt ccagagattc aagctccctg agatttgaaa aattacgggt
     4321 atggtttcct tttgccgtga tgaccagaga taatgaggct tcaccggaat caaccgttaa
     4381 cggtatcaga gcattatcat aaaactgctt attacaaaat tgaatgattt taggatgaca
     4441 acgataatgc tctttaagaa gggttactgg caccatgttc ctgaacaaca tacaaaccga
     4501 atcaagtaga ctatatttct cacaattata gtattctgcc ggtggagatg ggctgttcgg
     4561 gagtaataca ggaacatgtg gaagttgctt acgatcccct acaacgatta cattacgggc
     4621 acatcccagt cccagaatgc ccggaacaat atcctgctgc gaggcttcat caataataac
     4681 ataatcaaga agggccccct tgccgataga gttaataatc gaatgcgtac tgctaccaat
     4741 aatcggaaaa cgctttataa aacggtcaaa ttcatccctg tatgtctctg ccgagaatga
     4801 acttcttgtt gaaacatttc ttcgcagatg ttgctttaaa taaagcatag accatgatgt
     4861 caggcgcccc agtaaaattt taaaatcaga ttttttaaat gcttcctcat attcattcag
     4921 agaaatttgt ttttcccgga tatttttttc ataataagaa agctgaagag aagtaaatag
     4981 cgccagcctt ctctccggaa tcatcaatgg tttgactttc agtatcatga agtttaacaa
     5041 caaatcaatt ctgtttctga atccaatccg cctgtcagaa aggtaatgaa tataagccat
     5101 caggtcggtt gttttttgtg aggaaaatcg gtatttgtga gaaaaaagct cctccggtcg
     5161 aagattctga ctctgccatt gctgcaggta tttatactca atattcagct cattaatttc
     5221 tgcttttaac gaagcgacct ggttaatggc attcaggtgt tttttcactt gttgaagtac
     5281 atcatcaatc gcatttgcag acggtgagtc aggtaagact tcctcagaac gcgaaatcga
     5341 cgttgaaaaa aactgttgcc tgttctccgt actccccagt cgtgccatca catatcccag
     5401 ttgctgtttg tccatctttt cataaacatt actgacagct gagttattat tggacaaaat
     5461 tgccactgtt ttattctgaa ttaatatatt agcaacaata ttgagtatag tctgtgtttt
     5521 acctgttcca ggtggtcctt caataactga aatctgtgag gaaaatgccc gttcgacagc
     5581 cagcaactga ctttcattaa gaccaaacgg aaaaatcaaa ccccatggcg aatcacgttt
     5641 ttactctgtc ctgaacagta tgcatgcaag gcggtatccg gatgaggaac tattttcttt
     5701 atctgcctaa ggatattctc atcgatattt ctgttattac cacgggcata aaaaatacgt
     5761 tcattagcca ccctacacaa gtaattaaac acggcatgct ctgtaaatgc tgaacactgt
     5821 tgaaagtcaa ggccggtcgt tttcatgatg tagggtcgtt caccctcgcg gtaataaaca
     5881 gcagcatatt tatcaccata tataataacc agttcagccc gggatgttac agcgttccct
     5941 ttcctttcga ggaaaacatt tcgcaaattt acagttggcg ttatctgaca tacagaaagc
     6001 ggacgagtat attttctccc gctgcgaaaa tgacaggtca gcataacctc tctgtcgtca
     6061 tttccagccc acagcgtcca gtcggtaatt ttatgagtct tattttcacc gtcaacacaa
     6121 atcgtgacca tattcttact cagaaattat catttaactt acggaaaatt gttaaggcac
     6181 tcccgatatt
//
LOCUS       DAZFKY010000236         6174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFKY010000236 DAZFKY010000000
VERSION     DAZFKY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            219..278
                     /gene="ypfM"
                     /locus_tag="ACHKCL_004347"
     CDS             219..278
                     /gene="ypfM"
                     /locus_tag="ACHKCL_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5765183.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(377..3490)
                     /gene="acrD"
                     /locus_tag="ACHKCL_004348"
     CDS             complement(377..3490)
                     /gene="acrD"
                     /locus_tag="ACHKCL_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5765184.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(3654..5354)
                     /gene="narQ"
                     /locus_tag="ACHKCL_004349"
     CDS             complement(3654..5354)
                     /gene="narQ"
                     /locus_tag="ACHKCL_004349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5765185.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            5560..>6174
                     /locus_tag="ACHKCL_004350"
     CDS             5560..>6174
                     /locus_tag="ACHKCL_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5765186.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARK"
BASE COUNT         1545 a         1633 c         1558 g         1438 t
ORIGIN      
        1 tgttatctgt ctgtatttta accagaatta aatcagtatt agatgccgta tgaatatctc
       61 gaaatgttgc tgtgtatcac atacaaatca tcactgcagg accatagtat ttacataagg
      121 tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat tagcttcacc acggtatatt
      181 gtttccgtag aatacccata attataagag aggttgttat gattgaacgt gaactgggga
      241 actggaaaga ctttattgaa gttatgcttc gtaagtaatt cctgagacaa agtacaaaca
      301 gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa aaaggcgaca cattggcatg
      361 tcgccttttt tattgcttat tccgggcgcg gcttcagcgg gaagcggcgg cgcaccagca
      421 caaagaacag cggcacgaag taaatagcca gaatagtggc tgaaatcatc ccgcccatta
      481 cgccagtacc caccgcatgc tgaccaccgg aaccggcacc cgtgctggtt gccattggca
      541 atacgccgaa gataaatgcc agcgaggtca tcagaatcgg gcgtaaacgc tgacggcagg
      601 cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt catctcgtta gcaaactcga
      661 cgatcaggat ggcgtttttc gccgataaac caatgaccgt taacaggccc acctggaagt
      721 aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag cgcgccgatt acccccagcg
      781 gcacgaccag cattaccgag aacggtaccg accagctttc atacaatgca gccagacaca
      841 ggaataccac cagcaaggaa atggcgtaca gcgccggagc ctgcgcgccg gaaagccgct
      901 cctgatacga catcgccgtc cactccagac caaagccgtt tggtagctgc ttcactaacg
      961 attccataat atccatcgcc gtaccggtac tgacccccgg tgcggcttcc ccaacaattt
     1021 caaccgcaga ataaccgtta tagcgttcca gacgcggcga gcctgtctcc cagcgtgagg
     1081 tcgcgaaagc agagaagggc accatgccgc catctttatt tcggacatac cagagattga
     1141 tgtcatctgg cagcatgcga tacggcgcag ctgcctgcac atagactttc ttcacgcgac
     1201 cgcgatccat aaagtcattc acatagctcg aaccccaggc ggtttgcagt gtgtcgttaa
     1261 tatcgtcgat agcaacgccc agcgcctgag ctttacgctg gtcgatatca atctgcaact
     1321 gcggactgtc atcgaggccg ttatggcgca cacgggttag ctccgggttt tccgccgcca
     1381 gcgccagcaa ctggttacgt gctgccatca gcgcatcgtg acctgctcca gcgtggtcct
     1441 gcaactccat atcaaaacct gcagaactac caagaccgct aattgctggc gggctgctgg
     1501 cgataacgcg agcttcttta atttggttaa acgcctttgt tgcacgctcg ataatggcaa
     1561 acgaggtgcc ggtcttactg tcgcgttcgc tccagtcttt caggcggata aacattcgcg
     1621 ccacgttttg cccgttaccc ccagggccag aaccaacagt ggcaaacacc gacatgatgt
     1681 tgtctttttc atgggtgaag taatatttct cgatttgctc aacgactttc agggtctgtt
     1741 gttgggttga accgctgggc aattgtaccg aggtagtaaa catgccacgg tcttccagcg
     1801 gtaagaacga cgtcggcaaa cgcaggaaca ggaacaccat gccgccaagc agcaggacat
     1861 aaatcacaat ccagcgcagg ctacggtgga gaattttcgc cacccctttt tcgtagcgtt
     1921 cggcgttgcg gttaaacatc tggttaaacc aggcaaaaaa gcctttttgc ccgtgatgct
     1981 cacctttttt cagtggctta agtagtgtgg cacacagagc cggagtgagg atcatcgcta
     2041 ccagtactga cagcaccatc gccgcaacaa tggtaataga gaactggcga tagatggcac
     2101 cggtggtgcc gccgaagaag gccattggta caaataccgc cgaaagcacc atcgcaatcc
     2161 cgaccagtgc cccctggatc tgccccatcg atttacgcgt ggcttcgcga ggagtgagtc
     2221 cttcctcact cataatacgt tcgacgtttt ccaccaccac gatggcgtca tccaccagca
     2281 gaccgatcgc cagcaccatc gcgaacatgg ttaaggtgtt gacgctgtaa ccgaaggcgt
     2341 aaagtacgga gaaggttccc atcaacacca ccggcacggc gatagtgggt atcagcgtgg
     2401 cgcggaagtt ttgcaggaac agatacataa cgaggaaaac cagagcgata gcttccagca
     2461 gcgttttcac cacgtcttca atggaggctt taacaaacga ggtggtttca tacgccacct
     2521 tgtattccag tccgtgcggg aaatactgcg ccagctcgtc gagacgattg agcaccagct
     2581 ccgctgtcgc catttcgttc gcgccggagg ccagttttac ccccaacccg gaggctggct
     2641 taccattgaa gcggctaaga taatcgtatt tctccgcccc catttcggcg gtggcgacat
     2701 cgccgagcct tacttctgag ccgtcctgat tcacccgcaa ggtgatatcg cggaactgtt
     2761 ctggtgtttg cagcagtgac tgggcgttaa tggtggcgtt gagcgcctgc ttatcgacgg
     2821 aaggtgtacc accaagttgc ccaaccgcaa tctgcgcgtt ctgtgactca atggcatcag
     2881 tgacatcttt agctgtcatc tggaaactgt tgagtttcgc cgggtccagc cagatacgca
     2941 tggaatattg cgaaccatag gcatcgatat ccccgacgcc atttacgcgg cttaacgggt
     3001 cctgaatatt actggcaaca taatcagcaa tatcctgttt atccatcgaa ccatcggtag
     3061 agacgaaggc aatggtcaga atgttggtat cgccggtttt acgcacagtc acgccctgat
     3121 tttgcaccgc ctgcggtaac tttcgcatgg ctgattgcag ctggttttgt acttgctgca
     3181 cggcttcgtc cggatcggtg cctgctttaa aacttaaagt gacagatgcc tgaccggtgc
     3241 cactgctctg agatgacata tacatgagat tatcgaggcc ggtcatattt tgctcgataa
     3301 cctgggtcac ggtgttttcc agcgtctggg ccgatgcgcc gggatagtta gcggtcactc
     3361 gcacattcgg tggcgcgaga tcggggtatt gttcaacggg caatgaaaaa atcgccaggg
     3421 tacctgtcag acacaacagg attgccagca cccaggcaaa aatggggcga tcaataaaga
     3481 aattcgccat taaaagagga cctcgtgttt ctgcgtatcg ttgtaggctt gacttgcttc
     3541 actgtagcgg caaggtacga gccaatcgtg gagaaaaaaa ggagttaatg taaattatca
     3601 tgccctttta tactgaacct taactgcaag tattctttgg tcagtaggag gcattacatc
     3661 aactgacttt cctcaccctc cgcagagcga aagctaatac tcactaacgt gccgccgccg
     3721 gaaggttgtg aaaaagtcag cgtcccacct agccgttccg cgcgttcgcg catgatattc
     3781 agaccataat gaccttcggg ttctttcggt tcaccgatac caatcccgtt atcacggata
     3841 taaaccgtgt gattgccgtc cggcgcggtg acgcaactga cggcgatttc gctggcgttg
     3901 gcgtgcttca tcgcattcag caccgcttcg cgaataattt gcaacaaatg cacctgcatt
     3961 tgcgcatcca gtgccagggt tggcagacgg cagtcgaggg tcagtttggc gctggtttga
     4021 ttttgtaacg tatccagcat ttccctcaac gcggagggga gatccgcctg ctgcagcgtc
     4081 aggcgaaaag tagtcaacag ctcgcgtaac tgccgataag catcattcaa cgcctgggaa
     4141 aaatcggcca tgatactttg tgcggtggcg ttatcttccg gtatcgaacg cttaagtaac
     4201 gtcaactgga tacgtaagta agaaagtacc tgagccagcg agtcgtgcaa ttcgcgggcg
     4261 atggttgcac gttcttccat caacaataac tgctgaaaat gcttctgcgc ctgattaaag
     4321 tacaaaccgc gtcccagcat cgacgaaacg ctgttaagca gcggttctga tgatgaaacg
     4381 tgactatttt gccagtgcag ttcgccgtaa accgtctctt gcattgtcac cggtaaaatc
     4441 tgcatcggca attccgggtt tggttgccct tcgctaatcc gccagttttc accgacattt
     4501 aactccagat attcagccgc ttcattgtcg cgaacaatct gcaaaatatg gcggaaacaa
     4561 tgcacatcaa tctggctggt gttcagcgcc tgcgaacatt gatacaacac ctccagacga
     4621 cgcttggcct cgtggagatc gcgggtcttt tcttctactg acgcctccag cgaacggtac
     4681 aatttatgca gctcgctcga catctggtta aaggtttttg caagtagacc aagctcattc
     4741 ggcaggctgg tatccagcgg tggcgagtcg aactgtccgt gttcaatacg ctgactggcg
     4801 gtaaccagct gattcagcgg ggcaaccacc tgatggcgta tgcggcccaa agtaaaaaag
     4861 accagcgtga aaataccgat accgccagcc agggaaatcg ccaccaccag cagcattttg
     4921 cgttcagcgt agtgctgtaa agccagcacg aacaggtcta tctgattaac ataattatta
     4981 atattggcct gataccacgg caaatcgccc ttgctgagcc gattattcat ctccagccag
     5041 ttggcattca gatgcgcata gcgagttttt actgcttctg gcacatacca gacgttgagg
     5101 ttggttaata ccggtgaatg cagtgcctgc tgaaacagct ggcgatgtgc attgagttgt
     5161 ggagtgccac tttgcaagtc gtagcccagg cggtaactct gcatacgcag cgatccggca
     5221 atattgatag cctcagcgtc gcgcaaactg ctcgccagag ttagcagagc gatacccgtg
     5281 gaaagaatcg acagcagcac aatgtaaaaa aaggcccggg ccagactggc cgagacgggt
     5341 cgtttaacaa tcacacgcgt cttctccaca aaaaatcatt aatgttccag ttcgctgaaa
     5401 cgcaatttag ttagtgatgg gattatttga tctgccacag tttatagcga aatagttact
     5461 tattctgggc acatgaacat tgccgattcc cctgtaagtc gggtaataac aacactcata
     5521 taaagaataa ggtttttaca accaaaaaag aaggtcgtta tgaatcgttt tattatggcc
     5581 aacagtcagc aatgtctggg ttgtcatgct tgtgaaatcg cttgtgtcat ggctcacaat
     5641 gatgagcaac atgtcctgag ccaacaccat tttcatcccc gaattacggt tatcaaacat
     5701 cagcagcaac gtagtgcagt gacctgtcac cattgtgaag atgcgccctg cgcccgtagc
     5761 tgccctaatg gcgcaatcag ccacgttgat gacagcattc aggtcaatca gcaaaagtgt
     5821 attggctgta aatcctgcgt ggtggcctgt ccgtttggta cgatgcaaat cgtcctgaca
     5881 cccgtcgcag cgggcaaagt taaagccacg gcgcataaat gcgacctttg tgcagggcgc
     5941 gaaaacggtc ctgcctgtgt cgagaattgc ccggcggacg cgctgcagct ggtcactgac
     6001 gccgcactct caggcatggc gaaatcccgc cgcttgcgta ccgcacgtca ggaacatcaa
     6061 ccgtggcatg ccagtaccgc tgcacaagaa atgccggtaa tgagtaaagt cgaacaaatg
     6121 caggcaacgc ccgcgcgtgg cgagccggat aaactggcga ttgaagcgcg caaa
//
LOCUS       DAZFKY010000237         6119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFKY010000237 DAZFKY010000000
VERSION     DAZFKY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..301)
                     /locus_tag="ACHKCL_004351"
     CDS             complement(<1..301)
                     /locus_tag="ACHKCL_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO5765187.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGL"
     gene            complement(298..1968)
                     /gene="menD"
                     /locus_tag="ACHKCL_004352"
     CDS             complement(298..1968)
                     /gene="menD"
                     /locus_tag="ACHKCL_004352"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5765188.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2057..3352)
                     /gene="menF"
                     /locus_tag="ACHKCL_004353"
     CDS             complement(2057..3352)
                     /gene="menF"
                     /locus_tag="ACHKCL_004353"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5765189.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3431..3736)
                     /gene="elaB"
                     /locus_tag="ACHKCL_004354"
     CDS             complement(3431..3736)
                     /gene="elaB"
                     /locus_tag="ACHKCL_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5765190.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3791..4252)
                     /locus_tag="ACHKCL_004355"
     CDS             complement(3791..4252)
                     /locus_tag="ACHKCL_004355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5765191.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            4317..5234
                     /gene="rbn"
                     /locus_tag="ACHKCL_004356"
     CDS             4317..5234
                     /gene="rbn"
                     /locus_tag="ACHKCL_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5765192.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5422..>6119
                     /locus_tag="ACHKCL_004357"
     CDS             5422..>6119
                     /locus_tag="ACHKCL_004357"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO5765193.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHW"
BASE COUNT         1527 a         1658 c         1467 g         1467 t
ORIGIN      
        1 ccagcccctg gcaagcggcc atcatcgcca cccgtccgcc aagcgagtac cccaccagcc
       61 agaagctaag gatgttgtaa ctaaccaggg ttttacgcag taagtcggtg acatcatcaa
      121 atccatcgac gctaatcgcc gccgaaccac cgtgacctgg gagatcaaca tacaaccgtg
      181 agtagtcggc aaacgcctcg ccgacttcct gccattcgag gcaatcgccg gaaaaaccgt
      241 gcaaaaacac cagccagggt aaacctggtt tgccgtgttt tgcctgcgcg tgcaggatca
      301 taaatggctt acctgcgcca gcagctgctg gagcgtttgc gcgccgtcgg tgtcgttaac
      361 caccatttca attaccgtgg tggtcggagt acgccaggcg tcggcaagta ccgtttcaag
      421 ttcctgccag ttttgcggac gatgatattt cagctcaaac atcgcggcag cgtgctcaaa
      481 atggacgttt tgcggcatca gatagaaacg ctcgcgctcg cttttcggcg ttggcagcag
      541 cgagaaaatt tgcccgccgt tgttgttcac cacaattaat accagcggcg cggaaacctg
      601 gcgcaataac gccagcgcat tcaggtcgta aagtgcggag agatcgccca caatcgccag
      661 cgtcggtttg ccgcttgccc gctgaacgcc ggcggcagtc gagagcagcc cgtcgatacc
      721 gctggcccca cggttgctgt acaccgggta acctgccgga agttgcgaaa gcgcatcaat
      781 cagacgtacc accaggctgt taccgacaaa caattgcccc tgttcaggca gatagtcgct
      841 gatgcgatgc gccagttgcg cttcgccaaa cgcatcgcga caggcaataa ccgcctgcat
      901 tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc tggcgttttt ctgccggatg
      961 cagttccagc cagtcggcaa tattggcaat taagcggcgt ccacgatggt gtgccggatc
     1021 aagccgccct tcgatgtcat ccacaatcca gtactcttcg ggttcacagc ttgcctgcca
     1081 ttgcaggagc cgtttgcccg tcaggctgct tcccagttgc accacaattt gcgcctgttg
     1141 cagttcgctg gtcgctttgg cattgcctaa ccagagatcg gcacacggca gcggctgtcc
     1201 ggtttgcgac agcacgtcgc caatcagcgg ccagccaaga gtttgcgccc acagggcaac
     1261 ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct tttgccgcca
     1321 gaagaaccag tcgcgctgtt tttcgctctc aagacgaggc gcttcacgca gccacggctt
     1381 atcatcctgc caccagtcac ccagacgctg ttgccagcta agcccagtgt cgtccatctc
     1441 gccatacagc ggttcagcaa acgggcagtt gatatggacc ccaccagcat gaagcgtacc
     1501 gagagcatgg tcgatggtag aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg
     1561 cagtgaaata ctgtgcgtgg ggtgagaggc gaacattccc ggctgacgaa tcgcctgatt
     1621 cgcgccgcag tcaattagct ccggcgggcg atcggcggtt aacaggatca gtttttctcc
     1681 ggttaaccca gcttcaatca gtgccggata gagatttgct accgccgtgc cggaggtcac
     1741 aatcaccgcc accggctgct tgctgacttt cgccagcccc agcgccagat gccccagtcc
     1801 acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa
     1861 cggtgtagaa cgcgagccag gggcgatgca gatgtgtctt acgccgtgac gcgttaatgc
     1921 ttccagaatg accgccgccc agcgtcggtt aaatgcgctt actgacatga gtttgtccgg
     1981 tatcaatatt gcggctaagt ataaggagct acaaaaatag attattgata tgaatcggta
     2041 atgatgcgac tcattactat tccatttgta ataaagtacg cagccctgcc gctttgttgt
     2101 cgatttcctg ccactcttgc tcggggtcgg aaccacggac aatgcccgcg ccagcatata
     2161 gccgcacgac attgccgctg atttttgctg agcgcaggga aacgcagaat tcgctttgtt
     2221 gtaatgagag atagcccgca gaaccggcgt accattctcg ggtgaacggt tcgtgatggg
     2281 cgataaactg tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt ggctgcaact
     2341 gatgtaaaca gatcacatca tccgctttgt tgagtgaagt ccagatacag cggcgaagat
     2401 gctgcacttt acgcagacgc agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg
     2461 cctgtaatcg ttggcagata tcttccacca ccagcatgtt ctcgcgctgg tttttatcat
     2521 ccgccatcag ccactctcct aactgctgcg cctgcttatc atcaggatga tttgctactg
     2581 ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg ccgccataac cgttccggtg
     2641 aagagccaag aaaagcattt tcgccatcaa aggccatgta aaaatggtag caattgagat
     2701 tcagtcgacg actggcagcc atcatcgccg ccgcattgac cggacttgcg aaatgcaggt
     2761 cagttgcccg agcaagcacc actttgtcga gctcaccttc ggcgatggtt ttcgttgcca
     2821 gttcgattaa ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc gtagtggtta
     2881 aatgtaaccc aggcaagggc ttgatactca ccagtgtggc gataaattct cttgccttaa
     2941 tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc
     3001 cacagcgtcg ccattccagg cgtggtaaaa gtaaattgcc ctgcgacggg tcaaacgcat
     3061 tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct
     3121 ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc agcttcttca tcaccattac
     3181 gttgttgcca gtagaattgc gggtacgttt gctgactggc cagccagctc aaggcatcaa
     3241 aagcgtcttt gagagggaaa ggaatatcga taacccgaat gccgggtgtc gccggaatct
     3301 cttgcgacaa atggcgcagt agattttcca gcgccgtagt aagtgattgc acgcggacct
     3361 ctccctgtta aaagcctcac attatacggg gtactacaaa aaaaatgcag taccccggtg
     3421 tagggaggtt ttaacggcgt gccagcaaca gtcctagtac cagcccaacg gccgcaccca
     3481 caccaattcc ctgccagggt ttttcgtgga cgtagtcatc agcacgataa acagcctgct
     3541 tcgcccgata gtaataactg tctgaagcct ggctaacccg ttttttcaca tcatccagcg
     3601 ctttttctgc acgcgctttc agctcaacat atttctgatc ggcgggatcg ccagaggagc
     3661 ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac
     3721 caaactgatt agacattctc gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa
     3781 gggttattga ttacgcctga attacctcgc gtgccatccc aatgtgtggg ataccatctt
     3841 cttcatagat ctccgtcaca gggataaaac cgaaactctg gtagaagttt tgcagatgcg
     3901 cctgcgcccc gaggtagaca ggcttatcag gccagtgatg cgtgcaagtg tcgagggttt
     3961 tactcatcag ttgctgcccc actttttcgc cacgcaacgc ttcactgaca atcacccgac
     4021 caataacgac cggctcaaga tcatcatcac ttttcagaat cctcgcatat gccaccagtt
     4081 catcattttt ccaaccgaga atatggcgat tatcccccgt caaatcatca ccatcgatat
     4141 cctgataagg gcaattttgc tcgacaacaa acaccgcgca acgcagttgt aataaggcat
     4201 aaagctggga aaccgataat tcagagtgat gcagatcttg ccattcaatc ataatgctct
     4261 ccttttagat gtatgcctta tactagccgg aatatttttt gaaacgtgat gaactcatgg
     4321 aattaatttt tttaggtact tcggccggtg tgccaacccg cacgcgcaat gtcacggcaa
     4381 tattgctaaa cctgcaacat ccgacccaga gcggactttg gttgtttgac tgcggtgaag
     4441 gcacccagca tcagctactg cataccgcct ttaaccctgg aaaactggac aagattttta
     4501 tcagtcacct tcatggcgat catctttttg gtttacccgg cttgctgtgc agtcgttcta
     4561 tgtcaggcat tatccaaccc ttaacgattt atggtccgca aggtatccgt gaatttgtgg
     4621 agaccgcgct gcggattagc ggctcatgga ccgattatcc gctggaaatt gtcgaaattg
     4681 gcgctggcga aattctcgat gatggcctgc gcaaagtgac cgcttatcca atggaacacc
     4741 cactggaatg ctatggctat cgtattgaag aacatgataa accgggtgca ttaaatgccc
     4801 aggcattaaa agctgctggc gtaccgccta gcccactgtt tcaggaatta aaagcgggca
     4861 aaacaatcat gctggaagat ggaaggcaga ttaacggcgc agattactta gctgctccag
     4921 tgccaggtaa agcgctcgct attttcggcg ataccggccc ctgcgatgcc gcacttgacc
     4981 tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct ggatataacc atggaagcca
     5041 aagccaatag tcgcggtcat agctctacac gccaggctgc gacactggcc cgtgaggctg
     5101 gagtcggtaa gctaatcatt acccacgtca gctcgcgcta tgatgacaaa ggttgtcagc
     5161 acctgttacg tgaatgcagg tcaattttcc cggcgactga actagcgaat gatttcaccg
     5221 tgtttaacgt ttaactctgc ttattattaa aaacagggcg aaacttgccc tgttatcgca
     5281 acccgcgcta catatatcca ctcacacggg catattgagc gatttttgat atttattcat
     5341 ctccttacgc actatattca gagcaataag ttgcctcttt ttatcttcat tcagcacaac
     5401 ctttgcaact aaaaggagca tatgatggtt acagttgtca gcaattattg tcaattatct
     5461 caaacccaac tcagtcagac atttgcagaa aaatttactg tgaccgagga attactgcag
     5521 tctttaaaaa aaacagcgtt atccggagat gaagaaagca ttgagttact gcataatatt
     5581 gcgttaggtt atgatgaatt tgggaaaaaa gctgaagata ttctttacca tattgttaga
     5641 aacccaacaa atgataccct atcgattatc aagcttataa aaaatgcctg tttaaaatta
     5701 tataatttgg cacataccgc aaccaaacac cccctcaaat cacatgattc agataatctc
     5761 ctgttcaaaa aactattctc cccttcgaaa ttaatggcaa ttatcggtga ggacattcct
     5821 ctcatatcag aaaaacagtc actttcaaag gtacttttaa atgataagaa taatgaactg
     5881 agcgatggga caaacttctg ggataaaaat cgtcaattaa ccacagatga aatagcttgc
     5941 tatcttaaaa agatcgccgc caatgcaaaa aatactcaag tcaattatcc tactgatttc
     6001 tacctcccca attccaacag cacttacctg gaagtcgctc tcaatgataa tattaagagc
     6061 gatccatcat ggccgaaaga agtccagtta ttccccataa atactggcgg acactggat
//
LOCUS       DAZFKY010000238         6100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFKY010000238 DAZFKY010000000
VERSION     DAZFKY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHKCL_004358"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHKCL_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5765194.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKCL_004359"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHKCL_004359"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5765195.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1463..2005
                     /locus_tag="ACHKCL_004360"
     CDS             1463..2005
                     /locus_tag="ACHKCL_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5765196.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2105..3307
                     /locus_tag="ACHKCL_004361"
     CDS             2105..3307
                     /locus_tag="ACHKCL_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5765197.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3527..4309
                     /locus_tag="ACHKCL_004362"
     CDS             3527..4309
                     /locus_tag="ACHKCL_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5765198.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4324..5529
                     /gene="rhmD"
                     /locus_tag="ACHKCL_004363"
     CDS             4324..5529
                     /gene="rhmD"
                     /locus_tag="ACHKCL_004363"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO5765199.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5586..>6100
                     /locus_tag="ACHKCL_004364"
     CDS             5586..>6100
                     /locus_tag="ACHKCL_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5765200.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMG"
BASE COUNT         1454 a         1459 c         1589 g         1598 t
ORIGIN      
        1 ggcattttgc cagcatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cgctgggcag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtccg cgacctggtt ttcagcgggt
      481 aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcgatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taatcactaa
      721 attttcaatc tgacagccag taatggctgt catcattgtt acagaagacc tttcaaacgt
      781 aacgtttttc gggtggcgac attcaaatca tagtgcgcca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcaatcaaaa
      901 atcaggtaaa tcatataaat ttcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccaggg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagagc ctcttcaatt cgttcgccgg gctccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgcgatg ataactgcac ttattattat ttttgtgtgt atcggtaaac tgtgtttatt
     1321 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1381 tcgctcattg aatcctctgc gccaggcagt acaatcccgc agctttggga attttgatta
     1441 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1501 gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac
     1561 caatattggt gtcggttttg ggactgaatc gacgggcctg gctttaagcg gtaactggac
     1621 acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg
     1681 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1741 aggctatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1801 gtttggcgag tattactact ctccggattc actctccagc ggtattaaaa gttatgagga
     1861 agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg
     1921 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1981 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     2041 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2101 aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttacacg ggcaaatcgt
     2161 tgacactaac gctgcctggc tggccgattt tttctttcat caggggttgc cattatctcg
     2221 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2281 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
     2341 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     2401 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2461 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2521 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2581 gatggtcgag cacgaaatcc tgccgcgtct gcgcgagcgt ttttctttac cacagccgcc
     2641 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2701 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     2761 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2821 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2881 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2941 tttattggct ttgcaacttt ctcgcgcagg tgctccactg ctggcgtgtg aagtggttcc
     3001 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gagccaacca
     3061 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     3121 agccacgcca gatggcactt tagctctgcg tgtgcgtttc agcactacac gctatagcct
     3181 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctacgcc gttggttaaa
     3241 cggccaggac attgccagtg agcatggctg gattgaggtt gttgagtcca tgaccttatc
     3301 cgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3361 cttgtccaac ctgttgtact caataagaaa aaatccgtgc gctgattcac aaatttgtgc
     3421 gtaatttctc cagtccgttg gacgaggagc gggatttgat gcgcttcaca attttactcc
     3481 ccctgagcaa cgagactgat tccagtatct aagactggag tccagtatgt tggaatcaag
     3541 caaagttccc gccctgacac gggctattga tattctcaat ttgattgccc gcattggccc
     3601 ctgtaatgct gcgacgatca ttgacacatt ggggatccct aaaagcacgg cctatttgct
     3661 gcttaatgaa ctcaggcgtc agcgttttct tagccttgat caccaggaaa atttttgttt
     3721 gtggaccaag ctggtggagc tttccggtca tgcgttaagc aagatggatc tccgggaact
     3781 ggctcgaccg cgtttgacgc aactgatgga taccaccggg ctgttatgtc acctggggat
     3841 tattgataac ggcagcgctt attacatttt gaaagtggag tcatcagcca ctatcagcgt
     3901 gcgttcacat gaaggaaaaa gcctttcgct ttatcgctcc ggaataggta aatgcctgct
     3961 tgcctggcaa cctgcaactg tacagcaaag tattatcgaa gggctggtct gggagcaggc
     4021 gacacccaca acgattactc atccacagca acttcacgaa gaactggcgc gtattcgccg
     4081 tcagggctgg agctatgaca acggcgaaga ttacgccgat gtccgttgtg tcgctgcgcc
     4141 ggtgtttaac gccaataacg agcttaccgc tgcgatctcg gtggtcggta cccgcttaca
     4201 aatcaatgaa gaataccgtg attatctggc gggcaaggcc attgcctgcg ccagggatat
     4261 ttcacgtctg ttgggatgga aaagtccctt tgacttacaa gcctcataac aatggagaac
     4321 atcatgaccc taccaaaaat taaacaggtt cgcgcctggt ttactggcgg tgcgacagca
     4381 gaaaaaggcg ctggcggcgg tgattatcac gaccaggggg cgaatcactg gatagacgat
     4441 catattgcca ccccgatgag taaataccgc gattacgagc agttacgcca gtcatttggc
     4501 attaacgttc ttggcacgtt ggttgttgaa gtcgaagcag aaaacggcca gaccggattc
     4561 gctgtttcga cagccggtga aatgggctgt tttatcgtcg aaaaacacct taaccgtttc
     4621 attgagggta aatgtgtcag tgatatcaaa ctgatccatg atcaaatgct caatgccacc
     4681 ctgtattact ccggctctgg tggcctggtg atgaacacga tttcctgtgt cgatctggct
     4741 ctgtgggatc tgttcggcaa agtggtcggg cttccggttt ataaactttt aggcggcgct
     4801 gttcgtgatg agattcagtt ctacgccaca ggtgcgcgtc cggatctggc aaaagagatg
     4861 ggctttatcg gtggcaaaat gccgacgcac tgggggccac atgatggcga tgcggggatc
     4921 cgcaaagatg ccgctatggt cgcggatatg cgtgaaaaat gcggtgagga tttctggtta
     4981 atgctcgact gctggatgag tcaggacgtg aactatgcga ccaaactggc ccacgcttgc
     5041 gcgccctata acctgaaatg gatcgaagag tgcctgccgc cacagcagta tgaaggttat
     5101 cgcgaactga aacgcaacgc gccagtcgga atgatggtca ccagcggtga gcaccacggc
     5161 acactgcaat cttttcgtac gctttcagaa accggtatcg acattatgca gccggatgtt
     5221 ggctggtgcg gtggcttaac cacgctggtg gaaattgccg caatcgccaa atccaggggg
     5281 caactggtgg tgccgcacgg ttcgtctgtt tactcccacc atgcggtgat caccttcact
     5341 aatacgccat tcagcgaatt cctgatgact agtccggatt gttcaacgat gcgtccgcag
     5401 tttgacccga ttctgctcaa tgagccggtt ccggtgaatg gtcgtattca taaatcagtg
     5461 cttgataaac ccggttttgg cgtcgaactc aatcgtgact gcaatttgaa acgcccctac
     5521 agccactaat cactccgggc gttgctgtga caacggtaac gcccacatat tctaaggagc
     5581 tttttatgag caccgctttg cttgacgccg tggtgaagaa aaaccgtgtg cgtttaattc
     5641 cgtttatgtt ggcgctgtat gtgctggcgt ttctcgaccg ttcgaatatc ggttttgcca
     5701 aacagaccta ccagattgat accgggttga gtaatgaagc ttatgcgctg ggagcaggca
     5761 ttttctttgt ggtatatgcg tttctgggtg ttccggcgaa tcttttgatg cgcaaactgg
     5821 gggccagaac ctggattggt acgacaacac tgctgtgggg atttctttcg gcagccatgg
     5881 catgggccga tactgaagcg aaatttctga tagttcgcac tctgcttggt gctgcggagg
     5941 ccggattttt ccctggtatg atttatctca cctcgcaatg gtttccgcag cgtaatcgcg
     6001 ccagcattat ggggctgttc tatatgggcg caccgctggc gttaacactg ggatcaccgc
     6061 tttccggcgc gctgctggag atgcatggat ttatggggca
//
LOCUS       DAZFKY010000239         6097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFKY010000239 DAZFKY010000000
VERSION     DAZFKY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            219..866
                     /gene="fucA"
                     /locus_tag="ACHKCL_004365"
     CDS             219..866
                     /gene="fucA"
                     /locus_tag="ACHKCL_004365"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5765201.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            894..2042
                     /gene="fucO"
                     /locus_tag="ACHKCL_004366"
     CDS             894..2042
                     /gene="fucO"
                     /locus_tag="ACHKCL_004366"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5765202.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHKCL_004367"
     CDS             complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHKCL_004367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5765203.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHKCL_004368"
     CDS             complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHKCL_004368"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5765204.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHKCL_004369"
     CDS             complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHKCL_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5765205.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1544 a         1494 c         1617 g         1442 t
ORIGIN      
        1 ttttgataaa tatttttaag agcggtcatt tttaatgggg ttataaaaag agaacttgca
       61 gttatttact gtgattagtt gaactaggtc acaaaatata agcttgtgaa tttctttctt
      121 gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca
      181 acattattgc cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc
      241 gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg
      301 ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg
      361 aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa
      421 agctcccctc aagcgagtgg cgtttccata tggcggccta tcaaagcaga ccggatgcca
      481 acgcggttgt tcacaatcat gccgttcatt gcacagcagt ttccattctt aaccgaccga
      541 tccccgctat tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt
      601 atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta
      661 aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt
      721 tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta
      781 cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa
      841 cctatgggtt acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta
      901 acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg
      961 atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc
     1021 aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga
     1081 tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat
     1141 tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt
     1201 gtaaagcgat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag
     1261 gactttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca
     1321 ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg
     1381 tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg
     1441 gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg
     1501 ggtatattac ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa
     1561 tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg
     1621 cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt ggggttaggg ttggtgcatg
     1681 gtatggcgca tccactgggc gcgttttata acactccaca cggtgttgca aacgccatcc
     1741 tgttaccgca tgtcatgcgt tataacgctg actttaccgg tgagaagtac cgcgatatcg
     1801 cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg
     1861 ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg
     1921 gtgtacgcaa ggaagacatt ccggcactgg cgcaggcggc actggatgat gtttgtaccg
     1981 gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt
     2041 aaatgcgctg atgtgataat gccggatgcg acgtttgcgg cgtatccggc acgccgttac
     2101 cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca
     2161 atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg
     2221 gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc
     2281 gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca
     2341 agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc
     2401 gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga
     2461 cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc
     2521 gccagcgtgg cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg
     2581 cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc
     2641 ggtcgacccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca
     2701 tcgtcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca
     2761 tgctggcagg tttcgacaca gggcgacccc tgaacggcat gaatgcgacg aataagattc
     2821 agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa
     2881 gggtaacatt cgatactgga acaggctatg gctggatgag aaatcgggaa gaggcctcgc
     2941 aaaacgaggc ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg
     3001 cgagaggttt cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata
     3061 actttatcga ggcagacgcg cggctcgctg gtacggcgca gcgccatacg tgcggcgttc
     3121 accgctttta ctgccgcaat ggcgttacgc tcgatgcatg gcacctgtac ctgtccggcg
     3181 accgggtcac acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc
     3241 tgcgccgggc ttgcacctaa cagttctgcc agaccagccg ccgccattga gcacgccaca
     3301 ccaacttcac cctggcaacc cacttcggca ccagaaatcg acgcgttcat cttataaaga
     3361 gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg
     3421 ataaacttat cgtagtacgc cagcactgcc gggataatcc cgcacgcacc gttagtcggc
     3481 gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca ctgccagtgc aaacatgttg
     3541 atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg
     3601 cgtagtgccg cagcacggcg tggaacacgc agtttgccag gtaacacacc ttcggtggaa
     3661 ataccgcgct caataccgcc acacatcact tcccagacgt tcgccaggtg ctgttccagc
     3721 tcttctttgc tgtgcagcgc cagctcgttt ttcatcatca ggccagagag tgacagcccg
     3781 gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc
     3841 ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag
     3901 tagtaagtct gactgtaaac gactttatca cccgccagcg cggtaatgcg cataccgttt
     3961 tcatgcagag aaaggttgtc ggcatggaag ttcatgcact gatcaaccgg gaactccact
     4021 tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca
     4081 gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat aataatggcg
     4141 atatcagtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc aaccaccacg
     4201 cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct
     4261 ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat
     4321 acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc
     4381 gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg
     4441 acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt
     4501 gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc
     4561 atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc
     4621 gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta
     4681 atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg
     4741 atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta
     4801 gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc
     4861 aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg
     4921 aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca
     4981 acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc
     5041 agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt
     5101 tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat cagtacgcca
     5161 acaaacggga ataccagaat actcatcgct ttaacgatca tctgttcacc aaagcgaacg
     5221 atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg tggtgtcata
     5281 cccagctggt gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc
     5341 aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca
     5401 aagtgttctt ctacaacctc ggtaatgtct tcgcccgggt ttttaccaga cagtacgaag
     5461 cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc
     5521 agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt
     5581 gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag
     5641 tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca
     5701 aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct
     5761 gtctatcggc gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac
     5821 acaagttatg tatctatcag tgatcttgat cccaaataca ataatctact tggaagatag
     5881 attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg gcaatatttg
     5941 cccattaaac gacatctgac ttcttccggg gcgcgcaata tatgtaggtt gaactgaaaa
     6001 aatcaatact tttagttaat catggcgata atttcttttt catagcgata aaatacggtt
     6061 tatgaaaata gggaaacgtt tgcgttgcaa tataaat
//
LOCUS       DAZFKY010000240         6013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFKY010000240 DAZFKY010000000
VERSION     DAZFKY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            185..1288
                     /gene="ompC"
                     /locus_tag="ACHKCL_004370"
     CDS             185..1288
                     /gene="ompC"
                     /locus_tag="ACHKCL_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5765206.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1400..2455
                     /gene="apbE"
                     /locus_tag="ACHKCL_004371"
     CDS             1400..2455
                     /gene="apbE"
                     /locus_tag="ACHKCL_004371"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5765207.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2529..3593
                     /gene="ada"
                     /locus_tag="ACHKCL_004372"
     CDS             2529..3593
                     /gene="ada"
                     /locus_tag="ACHKCL_004372"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5765208.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3593..4243
                     /gene="alkB"
                     /locus_tag="ACHKCL_004373"
     CDS             3593..4243
                     /gene="alkB"
                     /locus_tag="ACHKCL_004373"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5765209.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4319..5962
                     /gene="yojI"
                     /locus_tag="ACHKCL_004374"
     CDS             4319..5962
                     /gene="yojI"
                     /locus_tag="ACHKCL_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5765210.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLSLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1430 a         1517 c         1633 g         1433 t
ORIGIN      
        1 tcgaatttac attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat
       61 catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg
      121 agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa
      181 taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc
      241 aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga
      301 cggcctgcac tatttctctg acgacaagtc tgtagatggc gaccagacct acatgcgtct
      361 tggcttcaaa ggtgaaactc aggttactga ccagctgacc ggttacggcc agtgggaata
      421 tcagatccag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg tggcattcgc
      481 aggtctgaaa ttccaggatg taggttcttt cgactacggt cgtaactacg gcgtagttta
      541 cgacgtaact tcctggaccg acgttctgcc agaattcggt ggtgacacct acggttctga
      601 caacttcatg cagcagcgtg gtaacggttt cgcgacctac cgtaacactg acttcttcgg
      661 tctggttgac ggcctgaact ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg
      721 cgaaggcatg actaacaacg gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg
      781 atctatcact tatgattacg aaggcttcgg tatcggtgct gcagtttcca gctccaaacg
      841 tactgatgat cagaacagcc cgctgtacat cggtaacggc gaccgtgctg aaacctacac
      901 tggtggtctg aaatacgacg ctaacaacat ctatctggct gctcagtaca cccagaccta
      961 caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt
     1021 tgctcagtac cagttcgact tcggtctgcg tccgtccctg gcttacctgc agtctaaagg
     1081 taaaaacctg ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt
     1141 tgatgttggc gcgacctact acttcaacaa aaacatgtcc acctatgttg actacaaaat
     1201 caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt
     1261 agctctgggt ctggtttacc agttctaatc tcgattgata tcgaacaaag ggcctgcggg
     1321 cccttttttc attgttttca gcgtacaaac tcagtttttt ggtgtactct tgcgaccgtt
     1381 cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc tggctgccgc
     1441 gctcttcttt gttggttgcg atcaaaaacc acaacccgcc aaaacccacg ctactgaagt
     1501 taccgttctt gaaggcaaaa ctatgggtac cttctggcgt gccagcatcc cgggcattga
     1561 cgccaaacgc agcgccgaac ttaaagaaaa gattcagacc cagctggacg ccgacgatca
     1621 gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt
     1681 atcgccgtgg ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tacgcattgg
     1741 cgcgaagacc gatggcgcga tggatataac cgtcgggccg ctggtgaatc tgtggggttt
     1801 cggtccggaa caacagccgg ttcaaattcc gagccaggaa cagatcgacg cgatgaaagc
     1861 caaaactggt ttacagcacc ttacggtcat taatcagtcg catcagcaat atctgcaaaa
     1921 agacctgccg gatttatatg tcgatctctc caccgtcggc gaaggttatg cggcggatca
     1981 cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc
     2041 gctgaacagc cgtggtatga acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc
     2101 aaccgataaa gaaaacgcgg ttcaggccgt ggtggatatc aacggtcatg ggattagcac
     2161 ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttccc atgttatcga
     2221 tccgcaaacc gggcgtccca tcgaacacaa tctggtatcc gtgacggtga ttgcaccgac
     2281 ggcgctggaa gccgacgcct gggatacagg tttgatggta ctcgggccgg agaaagccaa
     2341 agaagttgtt cgccgggaag ggctggcggt ttatatgatc accaaagaag gcgatagctt
     2401 taaaacctgg atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc
     2461 aagattgttg gtttttgcgt gatggtggcc gggcagacta aaggctatcc ttaaccaggg
     2521 agctgattat gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag
     2581 cccgcgaccc gaatgccgac ggcgaattcg ttttcgccgt gcgcaccaca ggcatctttt
     2641 gccgtccgtc ttgccgcgcc agacatgctt tgcgggaaaa cgtctccttc tacgcaaatg
     2701 ccagcgaggc actcgccgcc ggatttcgcc cctgcaaacg ctgccaacct gataaagcca
     2761 atgcccagca acatcggttg gataaaatca cccacgcgtg tcgactgctg gaacaggaaa
     2821 cgcctgtaac gttggaagcc ttagccgacc aggtggcgat gagtccattt catctacatc
     2881 ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc
     2941 gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt cttaacgccg
     3001 gattccccga cagcagcagt tactaccaca aagccgatga aacgctgggc atgacggcta
     3061 aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc
     3121 tgggtcgttg cctggtggca gaaagcgagc gggggatttg cgcgatattg ctgggcgatg
     3181 atgacgcgac attaatcagc gagttgcagc agatgtttcc cgctgccgac aacgcgcctg
     3241 ccgatctgac gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata
     3301 ctccgctgac gttaccgctg gatattcgcg gcactgcttt tcagcaacaa gtctggcagg
     3361 cactgcgcac gataccttgc ggtgaaaccg tcagttatca gcaactggct aacgccatcg
     3421 gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg
     3481 taattccctg tcatcgagtg gtccgtggtg atggcacact ttccggttac cgctggggcg
     3541 tgtcgcgtaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga
     3601 tctgtttgcc gatgctgaac cgtggcaaga gccactggcg gctggtgcgg taattttacg
     3661 gcgttttgct tttaacgctg cagagcaact aatccgcgat attaatgacg ttgccagcca
     3721 gtcgccgttt cgccagatgg tcacccccgg gggatatacc atgtcggtgg cgatgaccaa
     3781 ctgtgggcat ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc
     3841 gcaaacaaat aaaccgtggc ccgccatgcc gcagagtttt cataatttat gtcaacgtgc
     3901 ggctacggcg gcgggctatc cagatttcca gccagatgct tgtcttatca accgctacgc
     3961 tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccaat
     4021 tgtttctgtt tctctgggct tacccgcgat ttttcaattt ggtggcctga aacgtaatga
     4081 tccgctcaaa cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg
     4141 gctgttttat cacggtattc aaccgttgaa agcggggttt catccactca ccaccgactg
     4201 ccgctacaac ctgacattcc gtcaggcagg taaaaaagaa taaaaataag aattattatt
     4261 gctgtgcgcg cgaagatgtt taaactgcgg gctgtaattc attgtccggg ttttctgcat
     4321 ggaacttctt gtacttgtct ggcggcagta tcgctggcca tttatcagtg tgatggcgct
     4381 aagcctcgcc agtgcggctt taggcattgg cttaattgct tttatcaatc agcgccttat
     4441 cgaaacggcg gataccagtc tgctggtgtt gccggagttt ctgggattat tgctgctgtt
     4501 gatggcagtc actctcggat cgcaactggc gctcaccact ttggggcatc acttcgttta
     4561 ccgactgcgt agtgaattta tcaagcggat tctggatact cacgtcgagc gcattgaaca
     4621 actcggtagc gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata tcaccattgc
     4681 ttttgtgcgt ctgccggaac tggtgcaggg gatcattctc actatcggtt cagcggcgta
     4741 tctgtggatg ctgtcgggca aaatgttgct ggtaacggct atctggatgg cagtcaccat
     4801 ctggggcggt tttgtgctgg tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac
     4861 cgaagacaag ctgtacacgg attttcaaac tgtacttgaa gggcgcaaag agctgactct
     4921 gaaccgggaa cgcgccgagt atgtgtttaa caacctctac attcctgatg cgcaagagta
     4981 tcgccatcat attattcgcg cagacacctt ccatcttagt gccgtgaact ggtcaaacat
     5041 catgatgctg ggcgctatcg gcctggtgtt ctggatggcg aacagtctcg gttgggctga
     5101 taccaacgtt gccgcgacct attcgttgac gcttttattc ctgcgtacgc cgctgctttc
     5161 ggcggttggc gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa
     5221 attcgcgctc gcgcctttca aagcagagtt tccgcgcccg caggcgtttc ccagctggca
     5281 aacgctggag ctgcgtaacg tgacgtttgc ttatcaggat aacgcgtttt ccgttggtcc
     5341 gattaatctc accatcaaac gtggcgagct gctgtttctg attggcggca acggtagcgg
     5401 aaaatcgacg ctggcgatgt tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt
     5461 gctggatggc aaacctgtca gcggcgaaca accggaagat tatcgcaaac tgttttcggc
     5521 agtgtttacc gatgtctggc tgtttgatca actgctgggg ccggagggta aacccgctaa
     5581 cccgcaactg gttgagaagt ggctggcgca gctgaaaatg gctcataagc ttgagttaag
     5641 aaacgggcgt attgttaacc tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt
     5701 gctggcgctg gcagaagaac gcgatattat cctgctggat gaatgggcgg cggatcagga
     5761 tccacacttc cgtcgtgagt tttatcaggt gttgctgtcg ctgatgcagg agatgggtaa
     5821 aactattttc gccatcagtc atgatgatca ttactttatc cacgccgacc gcctgctgga
     5881 aatgcgcaac gggcaactta gcgagctgac gggcgaagag cgcgatgccg cttcgcgtga
     5941 tgccgttgcc cggacggcat aatattacga cgctgacctg gcggactgct gccgccaggt
     6001 caatatgcta aaa
//
LOCUS       DAZFKY010000241         5999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFKY010000241 DAZFKY010000000
VERSION     DAZFKY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            22..1092
                     /gene="ygjJ"
                     /locus_tag="ACHKCL_004375"
     CDS             22..1092
                     /gene="ygjJ"
                     /locus_tag="ACHKCL_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5765211.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            1109..3460
                     /gene="ygjK"
                     /locus_tag="ACHKCL_004376"
     CDS             1109..3460
                     /gene="ygjK"
                     /locus_tag="ACHKCL_004376"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO5765212.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            3986..>5999
                     /gene="fadH"
                     /locus_tag="ACHKCL_004377"
     CDS             3986..>5999
                     /gene="fadH"
                     /locus_tag="ACHKCL_004377"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5765213.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         1437 a         1625 c         1689 g         1248 t
ORIGIN      
        1 tttagctcat ggattaacac aatgaaatta atcactgcac catgcagagc attacttgct
       61 ctgccgtttt gctacgcctt ttctgcggca ggagaagaag cacgtccggc agaacatgac
      121 gacacaaaaa cccccgcaat tacctcgaca tcttctcctt catttcgttt ttacggcgaa
      181 ttaggggttg gtggatatat ggatttagag ggtgagaata aacataaata cagcgacggc
      241 acctatattg aaggtggcct ggagatgaag tacggctcct ggttcggcct gatttacggc
      301 gaaggctgga ccgtgcaggc cgaccacgac ggcaatgcct gggtgcctga ccatagctgg
      361 ggcggtttcg aaggtgggat taaccgtttc tatggcggtt atcgaaccaa tgatggcacc
      421 gaaatcatgc tcagtctgcg tcaggattcc tcgctggatg acctgcaatg gtggggcgac
      481 ttcacacccg atctgggcta cgtcattccc aatacccgcg acattatgac cgcgctgaag
      541 gtgcagaact taagcggcaa ctttcgttat agcgtcaccg ccacacctgc cggacatcat
      601 gacgaaagca aagcatggct acattttggc aagtacgatc gctacgacga caaatacacc
      661 tatccggcaa tgatgaacgg ttacatccag tatgaccttg ccgaaggcat cacctggatg
      721 aatggtctgg aaattaccga cggcacagga caactctatc tcacgggact gcttacccct
      781 aactttgccg ctcgcgcctg gcaccatacc ggacgtgccg acgggctgga cgtaccggga
      841 agtgaaagcg ggatgatggt gagcgccatg tatgaagcgt taaagggcgt ttatctctcc
      901 accgcttaca cctacgccaa acatcgccct gaccacgctg acgatgaaac cacctctttc
      961 atgcagtttg gtatctggta cgaatatggc ggcggacgtt tcgcaacggc ttttgatagc
     1021 cgcttctaca tgaaaaatgc ctctaacgat cccagcgacc aaatcttcct gatgcaatat
     1081 ttctactggt aataaggact gtaatttcat gaaaatcaaa actattttaa cgccagtaac
     1141 ctgcgctctg ctgataagtt tttccgccca tgccactaac gccgacaatt ataaaaacgt
     1201 gattaaccgt actggtgcgc cgcagtacat gaaggattac gattacgacg atcaccagcg
     1261 ttttaatccg tttttcgatc tcggagcctg gcatggtcat ctgttgccag acggcccgaa
     1321 caccatgggc ggctttccgg gcgttgcgct gctgacggaa gaatacatca actttatggc
     1381 cagcaatttc gaccgcctga ccgtctggca ggacggcaag aaagtcaact tcacgctgga
     1441 ggcatacaat attcccggcg cgctggtgca aaaactgaca gcaaaagatg tgcaggtcga
     1501 aatgactctg cgcttcgcca cgccgcgcac gtcactactg gaaactaaaa tcatcagcaa
     1561 taaaccgctg gatctggtgt gggatggcga actgctggaa aaactggaag cgaaagaagg
     1621 gaaaccgctt tccgataaaa ccattgctgg cgaataccct gactaccagc gcaaaatcag
     1681 cgccacccgt gatggcctga aagtcacctt tggcaaagtg cgcgccacct gggatctgct
     1741 gacctccggt gaatcagaat atcaggtgca taaatccctg ccggtgcaga ctgaaatcaa
     1801 cggcaatcgc tttaccagta aggcgcatat caacggttcg acgacgctct ataccaccta
     1861 ttcccatctg ctgacctctc aggaagttag caaagagcaa atgcagatcc gcgatattct
     1921 ggcgcgtccg gcgttttatc tcaccgcctc gcagcaacgc tgggaagagt atctgaagaa
     1981 agggttaacc aatccggatg cgacgccgga acagacacgc gtcgcggtga aagcgatcga
     2041 aacgctcaac ggtaactggc gctcgcctgg gggtgcggtg aaatataaca ccgtcacgcc
     2101 gtcggtgacc gggcgctggt tctccggcaa tcagacctgg ccgtgggata cctggaagca
     2161 ggcgtttgcg atggcgcatt tcaatccgga catcgccaaa gagaatatcc gcgcggtctt
     2221 ctcctggcag atccagcctg gcgacagcgt gcgtccgcag gatgtgggct ttgtccccga
     2281 cctgatagcg tggaatctta gccccgagcg tggtggcgat ggtggcaact ggaacgaacg
     2341 aaataccaaa cccagccttg ccgcctggtc ggtgatggaa gtgtacaacg tcacccagga
     2401 taaaacctgg gtggcagaga tgtacccgaa actggtggcc tatcacgact ggtggttacg
     2461 taaccgcgat cacaacggca acggcgtgcc ggaatatggc gcgacccgcg acaaagccca
     2521 caacactgag agcggcgaga tgctgtttac ggtgaagaaa ggcaacaaag aagagacgca
     2581 gtctgggctg aacaactacg cccgcgtggt ggagaaaggc cagtacgaca gtctggaaat
     2641 tccggcacag gttgctgcat cgtgggaatc ggggcgtgat gatgccgccg tctttgggtt
     2701 tatcgacaaa gaacagctgg ataaatatgt cgcaagcggc ggcaaacgta gcgactggac
     2761 agtgaaattc gccgaaaacc gcagtcagga cggaacgttg ctggggtact cgctattgca
     2821 ggagtcggtg gatcaggcca gctatatgta cagcgataac cattatctgg cggagatggc
     2881 gacgattctc ggtaaaccgg aagaggctaa gcgctatcgc cagttggcac agcagctcgc
     2941 ggactacatc aacacctgta tgttcgaccc ggctacgcag tacttctatg acgtgcgtat
     3001 tgaagataaa ccgctggcga acggctgcgc gggcaaaccg attgttgagc gcggtaaagg
     3061 gccggaaggc tggtcgccgc tgtttaacgg tgcggcaacg caggccaatg ccgacgcggt
     3121 ggtgaaggtg atgctcgatc ctaaagagtt caataccttt gttccgctgg gaacggcggc
     3181 gttaaccaat ccggcctttg gcgctgatat ctactggcgc gggcgcgtat gggtggatca
     3241 gttctggttt ggtctgaaag ggatggagcg ttacggttat cgcgatgatg ccctgaaact
     3301 ggcggatacg ttcttccggc acgccaaagg gttaaccgcc gatggcccaa ttcaggaaaa
     3361 ttacaacccg ctgacaggcg cacagcaagg cgcaccaaat ttctcctgga gtgccgcaca
     3421 tttttatatg ttgtataacg attttttccg taagcaataa tttgcaacgg ctggcggatg
     3481 cggcgtgaac gccttatccg ccctacatat tcgttcccgt aggctggata agacgcgaca
     3541 agcgtcgcat ccggcatctg cacctcgcca ccggcggatg ctgcgtgaac gccttatccg
     3601 ccctacatgt tcgttcccgt aggctggata agacgcgaca agcgtcgcat ccggcatctg
     3661 cacctcgcca ctggcggatg cggcgtgaac gccttatccg ccctacatgt tcgttcccgt
     3721 aggctggata agacccgaca agcgtcgcat ccggcgtctg caccgcgcca ctggcggatg
     3781 cggcgtgaac gccttatccg ccctacatgt gcaatccccc aggtcggata gggcccggca
     3841 agcgtcgcat ccggcgttac caccgggcgt attctttttg aatcccatca caaaccccgc
     3901 actccccttt tcccttttct ccggcgacgg ctaaattaga actcatccga ccacataaca
     3961 attattttac atactggaca attttatgag ctacccgtcg ctgttcgccc cgctggattt
     4021 aggttttacc acgttaaaaa accgcgtgtt gatgggttca atgcacaccg ggctggagga
     4081 atacccggac ggtgctgaac ggctggcagc gttttatgcc gaacgcgccc gtcacggcgt
     4141 ggcgctgatt gttagcggcg gcattgcacc agatttaaca ggcgttggca tggaaggcgg
     4201 cgcaatgctc aatgacgcca gccagatccc acaccatcgc accattaccg aagcggtaca
     4261 tcaggaaggc ggcaaaatag ccctgcaaat tttgcatacc gggcgctaca gctaccaacc
     4321 gcatctggtc gcaccgtccg cattgcaggc ccccatcaac cgtttcgttc ctcatgaact
     4381 cacccatgaa gagatcctgc aactgatcga cgatttcgcc cgctgcgcgc aactggcacg
     4441 ggaggcagga tacgacggtg tagaggtaat gggttccgaa gggtatttga tcaacgaatt
     4501 tctgacgctg cgcaccaatc agcgcagtga ccagtggggc ggcgattacc acaaccggat
     4561 gcggtttgcc gttgaagtgg tgagtgcggt acgcgaacgc gttggcaacg acttcattat
     4621 tatctaccga ctgtcgatgc tcgacctggt cgaaggcggc gggacttttg ccgaaacggt
     4681 agagctggcg caggccattg aagcggcggg cgcgaccatt atcaacaccg gcattggctg
     4741 gcatgaagcg cgtattccga ccatcgccac gcccgtgcca cgcggcgcat ttagctgggt
     4801 cacgcgcaaa ctgaaaggtc acgtctcgct gccgctggtg accaccaacc ggattaacga
     4861 tccgcaggtt gccgacgata ttctctcgcg cggcgatgcc gatatggtat cgatggcgcg
     4921 accgtttctt gcggatgcgg aactgctgtc aaaagcgcaa tcagggcgag ccgatgagat
     4981 caacacctgc attggctgca accaggcgtg tctcgatcag atcttcgttg gcaaggtcac
     5041 ctcgtgtctg gtgaatcctc gcgcctgcca tgaaaccaaa atgccgatcc ttcccgccat
     5101 gcagaaaaaa aatctggcgg tggtcggtgc gggacctgct gggctggcgt ttgccattaa
     5161 cgcggcggcg cgtggtcatc aggtaacgtt gtttgacgcg cacagtgaga tcggcgggca
     5221 gtttaatatc gccaaacaga tccccggcaa agaggagttt tacgaaacgc tgcgctatta
     5281 ccgccggatg atcgaagtga cgggcgtgac gctaaaactc aatcacaccg tgacggcgga
     5341 tcagttacag gcgttcgatg aaacgatcct cgccagtggg atcgtgccgc gcactccgcc
     5401 catcgacggg atcgatcatc cgaaggtatt gagttatctc gatgtactgc gcgacaaagc
     5461 gccggtgggc aacaaagttg ccatcatcgg ttgtggcggg attggttttg atacggcgat
     5521 gtatttaagt cagccgggcg aatccaccag ccagaatatc gccgggttct gtaatgaatg
     5581 ggggatcgac agcagcctac aacaggctgg tggcttaagc ccgcagggaa tgcagatccc
     5641 gcgtagccca cggcagattg tgatgctcca gcgcaaagcc agcaaaccgg gacaggggtt
     5701 aggcaaaacc accggctgga tccatcgcac caccctgctc tcgcggggtg tgaaaatgat
     5761 cccaggcgta agttatcaga agattgacga tgacgggctg catgtggtga tcaacggcga
     5821 aacgcaggta ttagcagtgg acaatgtggt gatctgcgca gggcaagagc caaaccgcgc
     5881 attggcgcag ccgctgattg atagcgggaa aacggtgcat ttaattggcg gctgcgatgt
     5941 ggctatggag ctggacgcac gacgggcaat tgcccaggga acacggctgg cgctggaga
//
LOCUS       DAZFKY010000242         5985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFKY010000242 DAZFKY010000000
VERSION     DAZFKY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..253
                     /locus_tag="ACHKCL_004378"
     CDS             <1..253
                     /locus_tag="ACHKCL_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5765214.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="ACHKCL_004379"
     CDS             265..843
                     /locus_tag="ACHKCL_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765215.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRFASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="ACHKCL_004380"
     CDS             840..1235
                     /locus_tag="ACHKCL_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5765216.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="ACHKCL_004381"
     CDS             1243..1983
                     /locus_tag="ACHKCL_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765217.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="ACHKCL_004382"
     CDS             1999..2421
                     /locus_tag="ACHKCL_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5765218.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="ACHKCL_004383"
     CDS             2403..2837
                     /locus_tag="ACHKCL_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5765219.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="ACHKCL_004384"
     CDS             2830..5379
                     /locus_tag="ACHKCL_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5765220.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="ACHKCL_004385"
     CDS             5376..5705
                     /locus_tag="ACHKCL_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765221.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>5985
                     /locus_tag="ACHKCL_004386"
     CDS             5705..>5985
                     /locus_tag="ACHKCL_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765222.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAE"
BASE COUNT         1394 a         1468 c         1955 g         1168 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcttt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcctg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaaga
//
LOCUS       DAZFKY010000243         5852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFKY010000243 DAZFKY010000000
VERSION     DAZFKY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            154..600
                     /locus_tag="ACHKCL_004387"
     CDS             154..600
                     /locus_tag="ACHKCL_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5765223.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            611..2050
                     /locus_tag="ACHKCL_004388"
     CDS             611..2050
                     /locus_tag="ACHKCL_004388"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO5765224.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2040..3110
                     /locus_tag="ACHKCL_004389"
     CDS             2040..3110
                     /locus_tag="ACHKCL_004389"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5765225.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3110..4858
                     /locus_tag="ACHKCL_004390"
     CDS             3110..4858
                     /locus_tag="ACHKCL_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO5765226.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4908..>5852)
                     /locus_tag="ACHKCL_004391"
     CDS             complement(4908..>5852)
                     /locus_tag="ACHKCL_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO5765227.1"
                     /translation="QPAAPAPEAKPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVY
                     IKCYNKLQIPVQRSLARYADWLKDFKQGPTGKESTVYGIYGISESNLAECEKGVKSAV
                     ALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNL
                     EAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQE
                     KFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYS
                     DWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1366 a         1491 c         1472 g         1523 t
ORIGIN      
        1 agatttattt tacctactaa tatgtgcctt acgccgcaaa atttatcctt cactctgctt
       61 tatattgtca cgacttcacg acatatccgc gctcatcaca aacccgcccc gctattacaa
      121 ttaactgaaa tattttttca tggagtccga ttaatggcag ctcttactgc aagctgtatt
      181 gacctgaata ttcagggcaa tggcgcttat tccgttctga agcagttggc gacaatggcg
      241 ttacaaaacg gttttatcac cgactcacac cagttcctgc aaaccctgct gctgcgcgaa
      301 aaaatgcact ccaccggttt tggttctggt gtcgccgtgc cgcacggtaa aagcacctgc
      361 gttaaacaac cgttcgtgtt attcgcccgc aaagcgcagg ctattgactg gaaagccagc
      421 gacggcgaag acgtcaattg ctggatctgc ctcggcgtac cgcaaagtgg cgaagaggat
      481 caggtcaaaa tcatcggcac actgtgtcgc aaaattattc accaggaatt tattcatcaa
      541 ctgcaacagg gcgataccga ccaggtgctt gccttgttaa atcaaaccct cagctcataa
      601 ggaagtggcg atggagtcat ccttacgtat tgtcgcgatc accaactgcc ccgccgggat
      661 cgctcacacc tacatggtgg cggaagccct ggaacagaaa gcccgttctc tcggccatac
      721 cattaaagtg gaaactcaag ggtccagcgg cgttgaaaac cgcttatcca gcgaagagat
      781 tgccgctgcc gattacgtga ttctcgccac cgggcgtggc ctgagcggtg acgatcgcgc
      841 acggtttgcc gggaaaaaag tttatgagat tgccatctcc caggcgctga aaaatatcga
      901 tcagattttc agtgaattac cgacaaattc gcagcttttt gccgcagata gcggcgtgaa
      961 gctaggcaaa caggaagtac aaagcggcag cgtgatgagc cacctgatgg cgggcgtctc
     1021 tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg gcgcttgcca acatgctggt
     1081 gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg ccgtcgttta cctgggtagt
     1141 tgaatccatc ggttaccttg gcttcacctt tatgatcccc atcatgggcg cttatattgc
     1201 ctcgtcgatt gccgataaac cggctttcgc tccggcgttt ctggtttgct atctggcgaa
     1261 cgacaaagcg ctgctcggca cccagtcggg tgcgggattc ctcggcgcgg tggtgctggg
     1321 gctggcgatt ggctatttcg tcttctggtt ccgtaaagtc cgtcttggca aagcattaca
     1381 accgctgctc ggctccatgc ttatcccatt cgttactctg ctggtttttg gcgtgctcac
     1441 ttactacgtt atcgggccgg tgatgtccga cctcatgggc gggctactgc acttcctgaa
     1501 taccattcct ccatcaatga agtttgcggc agcgtttctg gtcggtgcga tgctggcgtt
     1561 cgatatgggc ggccccatca ataaaaccgc ctggttcttc tgcttctcac tgctggaaaa
     1621 acacatttat gactggtacg ccatcgtcgg cgttgtcccc gtcgcggcgg gtcttgcgac
     1681 gttcattgcg cccaaactgt ttacccgaca ggaaaaagag gctgccagta gtgccattgt
     1741 ggtcggtgcc acagtcgcaa ccgaaccggc tattccttac gctctggcag ctccgttgcc
     1801 gatgattacc gccaatacgc tggcgggcgg cattaccggc gtgctggtga tcgctttcgg
     1861 aatcaaacgt ctggcacctg gtttgggtat ctttgacccg ctgatcggcc tgatgtcgcc
     1921 ggtaggttcg ttttatctgg tgctggcgat tggcctcgcg ctgaacattt cattcattat
     1981 cgtcctgaaa ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca
     2041 tgaacattga gttactgcaa cagttgtgcg aagccagcgc cgtcagcggc gatgaacagg
     2101 aagttcgcga cattctgata aacacgctgg aaccttgcgt taatgagatc acctttgatg
     2161 gtctgggcag ctttgttgcc cgtaagggaa ataaaggtcc aaaagttgcc gttgtcgggc
     2221 atatggatga agtcggcttt atggtcaccc acatcgacga gagcggtttt ctgcgcttta
     2281 ccaccattgg cggctggtgg aatcagtcga tgctcaacca ccgggtaaca atacgcacac
     2341 acaagggagt gaaaatccct ggtgtgattg gttccgtagc gcctcatgcg ttaacggaaa
     2401 agcaaaagca acaaccgctg tcatttgatg agatgttcat tgatattggc gcgaacagtc
     2461 gcgaagaagc gaaaaaacgc ggtgcggaaa ttggcgattt tattagcccg gaagccaatt
     2521 ttgcctgctg gggcgaagat aaaatagtcg gcaaggcgct ggataatcgc atcggctgcg
     2581 cgatgatggc tgagctacta cagacagtaa ataacccagg aattacgctg tacggcgtcg
     2641 gcagcgtgga agaagaagtt gggctacgcg gggcacaaac ctcggctgaa cacactaaac
     2701 cggatgtggt gatcgtgctg gataccgccg tcgcgggcga tgttccgggc attgataaca
     2761 ttaaataccc gctgaaactg ggcaacgggc cggggctgat gctgtttgac aagcgctact
     2821 tacccaacca gaaactggtg gcggcgttaa aaaactgtgc cgcacataac ggtttaccgc
     2881 tgcaattttc caccatgaaa accggagcga cggatggcgg gcgctacaac gtaatgggcg
     2941 gagggcgtcc ggttgtcgcg ctgtgtctgc caactcgtta tctgcacgct aacagcggta
     3001 tgatttcaaa agccgattat gatgctctgc tcacgctgat acgggatttt ctgacgacct
     3061 taactgcgga gaaagtcaac gcgtttagcc agttccgtca ggtggattaa tgctaaacga
     3121 acgccagtta aagattgtcg atctgctgga gcaacagccg cgcacgcctg gcgagctggc
     3181 gcaacagact ggcgtttcag gcaggaccat cctgcgtgat attgactatc tcaactttac
     3241 ccttaacggc aaagctcgta tctctgccag tggcagtgcg ggctatcagt tggaaatctt
     3301 cgagcgccgc agcttttttc agttgctgca aaagcacgat aacgacgatc ggctgctggc
     3361 gctgttatta ctgaatacct ttactccccg tgcgcaactc gcctcggcgc ttaatttgcc
     3421 agaaacttgg gtcgcagagc gtctgccccg gttaaaacag cgttatgaac gcacttgttg
     3481 cctggccagt cgtcccggtt tgggccattt cattgatgaa acagaagaga aacgcgttat
     3541 cttgctggcg aacttgctac gcaaagatcc gtttttaata cctttggctg gcgtaacacg
     3601 agacaacctt cagcatttat ccacggcctg cgacaaccaa caccgctggc cgctcatgca
     3661 gggggattat ctctccagcc tgattctggc gatttacgcc ctgcgtaatc aactgaccga
     3721 tgagtggccg caatatcccg gtgacgagat aaaacaaatc gttgaacata acggtctgtt
     3781 tcttggtgat aacgctgtaa gaaccctgac aggtttgata gagaaacagc atcagcaagc
     3841 gcaggtaatt tctgccgatc atgtgctggg gttgctgcaa agggtgccag gtctcgcgtc
     3901 attgaatatt attgatacgc agctggttga gaacattacc ggtcatttat tacgttgcct
     3961 tgccgcacca gtgtggattg ctgagcatcg ccaaagcagc atgaataacc tgaaagccgc
     4021 ctggcctgcg gcctttgata tgagtctgca ctttattacg ctactgcgtg aacagctcga
     4081 tattcccctt ttcgacagcg atctgatcgg tttgtatttt gcctgtgcgc tggaacgaca
     4141 tcaaaacgaa cgccagccga tcattttgct ctcggaccag aacgcgattg ccactattaa
     4201 tcagctcgcc attgagcgcg atgttttaca ttgtcgggtg attattgccc gtagcttaag
     4261 cgaacttgtt gccattcgcg aagagattga gccgttattg atcattaaca acagccatta
     4321 tttactggat gacgcggtaa ataattacat caccgtaaaa aatatcatta cggctgccgg
     4381 tatcgaacaa ataaaacatt ttctggcgac ggcatttatt cgccagcaac cggagcgttt
     4441 tttctctgcc cccggaagtt ttcattattc gaatgtacgc ggtgaaagct ggcaacatat
     4501 tacccggcaa atttgtgcgc aattagtcgc acaacaccat attaccgccg atgaagcgca
     4561 acggatcatc gcccgcgaag gcgaaggtga aaacctgatt gttaatcgcc tcgccatccc
     4621 acattgctgg agcgaacagg agcgacgttt tcgtggattt tttattaccc tcgcccaacc
     4681 agttgaggtg aataacgaag tcattaacca tgtcttgatc gcctgcgccg ccgccgatgc
     4741 gcgtcatgag ctaaaaatat ttagctatct ggcaagcgta ttgtgtcagc atccggcaga
     4801 agttattgcc gggctaacag gatatgaggc atttatggag ttacttcaca aggggtgaac
     4861 tgtgtcggat gcgccgcatc cgacactgca tttatcatca aaaacgttta acgcaggcta
     4921 ttatagtcat caaccatttc gttgtactcg cgtaacgctc gtggaaatga gtcttcgacc
     4981 atccagcttg tgtttgcatc ctgaagatgc tctttatccc agtcagagta cgggacttta
     5041 tctctgacgc ggcgaacata ttttttagtc tcgagttgat actggtctgc cgaattaatg
     5101 aacgagaaat tcatgccgcc tttatccgct tctttggcct gcgccaccag cgtttccagt
     5161 tcagacactt tcttcattgc cgcatcaaca tcaaacttct cttgcgatat caggttatta
     5221 atttgtttcg ctgaaatcat gactgccaga gaatagtagt ggaatgtttt cccttctctt
     5281 tcttcaatat ttttcagttc cgcaagctgg cgcttatcat taatttcctg aatcgccgca
     5341 tgataagatt ctgctacagg ttcaaaggct tccagatttt ttaagaatgt ctggtgcagc
     5401 gttttccctt tcgcaaacgc atcgtcttta taattttcct gggtgtaata tttatccatt
     5461 tcgttaatgg tattacccaa cgccacggca gcatcgatat aactcaccgc cacgccatca
     5521 attggttgca gcgcaggcgt taacgccaca gcacttttta cgcctttttc acactcagcg
     5581 aggttggatt cactaatgcc gtagatgcca taaacagtac tctctttacc ggtaggcccc
     5641 tgtttaaaat ctttcagcca gtcagcataa cgcgccagac tgcgctgtac cgggatctgt
     5701 aacttgttgt agcacttgat atagacatcc attttttcgt cgaaaaccgc ttgttcatcg
     5761 accagcggtt gcgcaggtgt ttcaggttta gcatctgctt ttgcaacggg agcttcagta
     5821 ggtttcgctt caggagctgg tgcggcaggt tg
//
LOCUS       DAZFKY010000244         5481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFKY010000244 DAZFKY010000000
VERSION     DAZFKY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..122
                     /locus_tag="ACHKCL_004392"
     CDS             <1..122
                     /locus_tag="ACHKCL_004392"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5765228.1"
                     /translation="INVFLRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            194..1552
                     /gene="mltD"
                     /locus_tag="ACHKCL_004393"
     CDS             194..1552
                     /gene="mltD"
                     /locus_tag="ACHKCL_004393"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5765229.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1600..2370)
                     /locus_tag="ACHKCL_004394"
     CDS             complement(1600..2370)
                     /locus_tag="ACHKCL_004394"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5765230.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2448..3248)
                     /gene="yafD"
                     /locus_tag="ACHKCL_004395"
     CDS             complement(2448..3248)
                     /gene="yafD"
                     /locus_tag="ACHKCL_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5765231.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3489..4403
                     /gene="yafC"
                     /locus_tag="ACHKCL_004396"
     CDS             3489..4403
                     /gene="yafC"
                     /locus_tag="ACHKCL_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5765232.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4400..5203)
                     /gene="dkgB"
                     /locus_tag="ACHKCL_004397"
     CDS             complement(4400..5203)
                     /gene="dkgB"
                     /locus_tag="ACHKCL_004397"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5765233.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5366..5442)
                     /locus_tag="ACHKCL_004398"
     tRNA            complement(5366..5442)
                     /locus_tag="ACHKCL_004398"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5406..5408),aa:Asp,seq:gtc)
BASE COUNT         1444 a         1313 c         1358 g         1366 t
ORIGIN      
        1 aaattaatgt ttttttaaga acggaagata ttgatttaat taatgtaatt aatgaagaaa
       61 cattattgca acaacctgaa gagcgttttg catggttaag gtcaaagaaa gataggttct
      121 gataaaactt tcttgtcatc ggctccgttc gccgttatga tcggtcgtct tttaagcaac
      181 tattgacgca cacatgaagg caaaagcgat attactcgcc tctgtcctgc tcgtgggttg
      241 ccagagtacc ggcaacgttc aacagcacgc acagagcctt tctgcagctg gtcaagggga
      301 agcagcaaag tttacaagtc aggcacgatg gatggacgat gggacgtcta tcgcgccaga
      361 tggtgacttg tgggctttca ttggcgacga gctaaagatg ggaattccgg aaaatgaccg
      421 gattcgcgaa cagaaacaga aatatttacg caataagagc tatctccacg atgtaacttt
      481 acgggcagag ccgtatatgt actggatagc cgggcaagtt aaaaaacgta acatgcctat
      541 ggaactggta ctactaccca tagtggagag cgcttttgat cctcacgcaa cgtctggcgc
      601 caatgccgca ggcatctggc agatcattcc gagcacgggg cgcaattatg gtttgaaaca
      661 gacccgcaat tatgacgcgc gtcgcgatgt tgttgcttca acaactgccg cgctgaatat
      721 gatgcagcgt ctgaacaaaa tgtttgatgg cgactggctt ctgaccgtag cggcttataa
      781 cagcggcgaa ggtcgggtca tgaaggcaat taaaacgaac aaagcgcgtg ggaaatccac
      841 ggacttctgg tcgttaccgt tgccgcagga aacgaagcag tacgtgccta aaatgctggc
      901 attgagtgat attctcaaaa acagcaagcg ttatggcgta cgtctgccaa cgaccgatga
      961 aagccgtgct ctggcgcgtg tgcacctgag tagcccggtt gaaatggcga aggttgcaga
     1021 tatggcgggg atttccgtca gcaagctgaa gacattcaac gctggcgtga aaggctccac
     1081 gctgggcgca agtggtccgc agtacgtgat ggtgccaaag aagcatgcag atcaactgcg
     1141 tgaatctctg gcttcaggcg aaattgctgc tgtacagtcg acgctggttg ccgacaatac
     1201 gccgcttaac agccgtgttt acaccgtacg ctctggcgac acgctttcaa gtatcgcttc
     1261 acgtctcggc gtaagcacca aagatttgca gcagtggaac aaactgcgcg gctctaagct
     1321 gaaaccaggc caaagtctga cgattggcgc aggtagtagc gcacagcggt tggcaaacaa
     1381 cagcgatagc attacgtatc gtgtgcgcaa aggcgattcg ctttcaagca ttgctaaacg
     1441 ccacggcgtg aacatcaaag atgtgatgcg ctggaacagc gatactgcga atctgcaacc
     1501 aggcgataag ctgacgttgt ttgtgaaaaa caacagcatg ccagactcct gacaaaccag
     1561 ataatagaaa ggcaccgatt cccccggtgc cttttttatt tatgccgctt tatgtgcatc
     1621 taccatgatg atatcactgg tgaaagagcc atcattctgc aaggcaaaat acgttctcac
     1681 ctctgtcgat gcgctctgtt ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg
     1741 cattctcgcg acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac
     1801 tatcagattg gcttcattga ttaacctcaa ccactcaccg ctggcgtagt ttcgtacgtg
     1861 agaggtatcg cataatgctt ctaccgtctg taaccagatg tcgcgcactg ggtggcccgg
     1921 agacattacg tccatcacaa tcagcctacc gccaggtttc aatatccggt tcacttcacg
     1981 cagtgctgca ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa
     2041 tgcgttatcg gcaaatggca gactttcggc atacccctgg cgggtggtga tatttttcag
     2101 ttgccggact tcggcagctt gtgccacgac atccagcatt tgggcagata agtcatacgc
     2161 caccaccgtg ctcacatttt gcgcagcgac aaagctggca tgccctgctc cgcagcccat
     2221 atcaagtaca cttgcatcag gataatcagc cagacacacc gccaggagct gtaaatctcg
     2281 tccggatgca tgtacggtac tggtcagata ttcgctggct tgagagctaa actgcttttc
     2341 tacgtggtca tggtgggatt gtgttgtcat tgcactgtcc ttttgttgtt tgagtataaa
     2401 agggcagctc caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc
     2461 ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg acgcgcgcgt aaccagtaca
     2521 gaagcttcgc tgacgttcag accacggtag aaaacaaaat cgagcgggcg accaaacgcc
     2581 cgacggcgct gatcatcggt aaaacgcacc tgacgcagcg acatttcccg cgcaaaacga
     2641 tataacgcgt tcatccttct acggctccaa gcattgaaat ctcccgccat aatgaccggg
     2701 ccgctgtggt gagctatctg atcaccaata ggaagtaact gcttactata aacatccacg
     2761 cccagactga agttgacggc gtgtatatta accaccatta acaggcgggt gtcaggtaat
     2821 ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac
     2881 gggcagcaat acactggatg tgccgccgaa agggtcatta cgccagaagg atgttgtggc
     2941 agcacgaaag cgggtacctg atcggcggca agatagttag cagtcgcaaa ctgtaccaac
     3001 tctggcgttg tttgcgcttc ctgcaataac accagatgtg cgtctttgcc gtaattcttt
     3061 aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata
     3121 cgctcttcgg tacttaacgg ttctccgggt ggtaatgcct ggccgatgct cgcaaaagac
     3181 cccggcggta agatcctctc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt
     3241 tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc
     3301 tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc
     3361 aagtgcttac accggtcaga attttgctca ttattgagaa cattaaatcg gatgacatat
     3421 cacgcatcct gatggtttaa tgacaaataa attcaaaata tctcatctat tttgcaacag
     3481 gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtcgaaagcg
     3541 gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg
     3601 tgaaaaagct ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta
     3661 gcctgacgga agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg
     3721 cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg ttgcgcatcg
     3781 atgccgcaac tccagtggtg ctgcactttc tgatgccgtt gattaagcct tttcgtgaac
     3841 gctatccgga agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa
     3901 gaaaagtgga tgtcgcgata cgcgccggta cgttaacgga ttccagctta cgtgccaggc
     3961 cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga
     4021 agccagaaac gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt
     4081 ccctcaatac ctggccggta gcctgtagcg atggacaatt acatgaggtg aagtacggtt
     4141 tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt
     4201 gtttgtccga ctatatgatc gacagagaaa tcgctcgcgg agaattggtg gagttaatgg
     4261 cagataaagt gttgccagtg gaaatgccat tcagtgccgt ctattacagc gaccgtgcgg
     4321 taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg
     4381 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
     4441 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
     4501 gtgccttaag attactctcc aggttttcac gtttagtaga agaaggaatt actgagtaac
     4561 cttcccccat agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga
     4621 tacgagcaat aacctcatct ttcagggcct taccatacgc cagcgtcata taggaagtaa
     4681 tatggatgcc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
     4741 gttcaatctg gttagtagcg atgttttcag cgccaacagc agcaatcgcc ttttccatca
     4801 atgggatcgt gaagttggaa ataccgatct cacgcgtcaa cccttctttt ttggcttcca
     4861 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
     4921 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctcttttaga ctcgggatca
     4981 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
     5041 gcacgccact ttctgcaatc gccagaccta ctgcggcttc gttatcatag atttgtgcag
     5101 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
     5161 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
     5221 gctattttcg ttaactgagg tgattatggg ctttagaatg ttgcggaaaa agggcgtaaa
     5281 acgcagagga tttttgcgat tttagcaata atagaaatgt agacgaaaaa aaacctcgag
     5341 cgataactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     5401 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     5461 caccggtttt atgaccggct t
//
LOCUS       DAZFKY010000245         5301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFKY010000245 DAZFKY010000000
VERSION     DAZFKY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..206)
                     /locus_tag="ACHKCL_004399"
     CDS             complement(<1..206)
                     /locus_tag="ACHKCL_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5765234.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDA"
     gene            complement(190..432)
                     /locus_tag="ACHKCL_004400"
     CDS             complement(190..432)
                     /locus_tag="ACHKCL_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5765235.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            824..1162
                     /locus_tag="ACHKCL_004401"
     CDS             824..1162
                     /locus_tag="ACHKCL_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5765236.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1455..1607
                     /locus_tag="ACHKCL_004402"
     CDS             1455..1607
                     /locus_tag="ACHKCL_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5765237.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            1619..2257
                     /locus_tag="ACHKCL_004403"
     CDS             1619..2257
                     /locus_tag="ACHKCL_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765238.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2258..2467)
                     /locus_tag="ACHKCL_004404"
     CDS             complement(2258..2467)
                     /locus_tag="ACHKCL_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765239.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            3032..3220
                     /gene="dicB"
                     /locus_tag="ACHKCL_004405"
     CDS             3032..3220
                     /gene="dicB"
                     /locus_tag="ACHKCL_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5765240.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            3217..3405
                     /locus_tag="ACHKCL_004406"
     CDS             3217..3405
                     /locus_tag="ACHKCL_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5765241.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            3498..4736
                     /locus_tag="ACHKCL_004407"
                     /pseudo
     CDS             3498..4736
                     /locus_tag="ACHKCL_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            4724..5275
                     /locus_tag="ACHKCL_004408"
                     /pseudo
     CDS             4724..5275
                     /locus_tag="ACHKCL_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1551 a         1194 c         1209 g         1347 t
ORIGIN      
        1 gcatcagggg tgtctttctc tatccagcgg aaaattttct gggtattgcg agccagggct
       61 tccggatggc tgtcgtcgta cagttcagga aacgtcatac ccaactcaaa ataagcctgg
      121 gttattccag ctgccggaac tttttcgcca tcaggacgcg cccaggcatt catcgccatg
      181 cggatgtgtt catgcttgat tttcatgaat ccccccttgg ttagaaggcg gattatgatc
      241 agaatcggga atgacaaccg tcggtatgtg taactcatat ttgagcgccc cggcagtgac
      301 tgcctgaatt agcaacgccc atttccacgg aacgtcttcc ccccacatgc tgactgtggt
      361 ttttgacgtt cctagagcgg cggctgtttt aacaactccg ccaaaatagc ctaatacttc
      421 tgattttttc atgagtcgct ccataaaact gaacgccaaa agtttaataa tcaaaaccaa
      481 agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat
      541 atctgaacgc ataacccaac gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa
      601 tgccactggc gcatcaaaag gctccgtaag tcaatggatg aacggtggag gagcgccgtc
      661 ctcgcgttac ataagttcac tggcaaagat attgaaagta aacgaaaatt ggcttcttaa
      721 tggaggagag ttaaatacag gtgattcgct tgatctatct ttaccgccga taaaaacggt
      781 tccgctacta tcacttcagc aggcagcaag ctggagtgat tatatgaaaa attcctcaat
      841 aacctcttgt gtgcagcttg tcggagaaat cccggccaat acctttgctg ttgttctaga
      901 gagtgacagt atgtcgacat ctggtggtgg agtttccatc ccaaatggtt caacagtttt
      961 tgttgatccc gatcgaatcg tacaaccagg aaatattgtc cttgccttac ccaaagggac
     1021 cacaacgcct gtcattcgca aactggagat agaagggccg gatattcttt tagtccccac
     1081 gaatcctcgc tacccttcaa ttatgctgga tgatctatct tgcatattgg gagtatgctt
     1141 taaaattcaa caagatattt aaccaacctc atctatttga ttaactgtat gccatcgtag
     1201 tgatggcgta acagctgcct gcttaaaatg ttttgataaa aaaacattga ccttaaatgt
     1261 tcgtttttct aaacttcatt cattccctca ccccgcccca cagaatgcag ggcaatactt
     1321 cgagttacca ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
     1381 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
     1441 gaaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg
     1501 tgttccccaa ccaggacggt acgttcaccg cgatgacgta taccaaaagc aaaacgttta
     1561 aaactgaagc tggcgcgcgt cgctggttag ccagaaacac tgactgatga ggttgacgat
     1621 ggaatttaaa gatttacctc cttcaatcca ggagattgca gcacacacac ttcgtcatcg
     1681 tctgaacgaa cttgaattgg aatcggtaac gaaaaaagac actgataata tggctcgtaa
     1741 tgtgcgcgat gcgtttaccg gattgtattt ctgtgcgtct ataaataaac acgactcaga
     1801 gagtgtggca aataaaattg cagaaacgac agcgcaaaac atcaatacga aaccaacgga
     1861 agaagaaatt gatcagtttg ctcatgatgc tggtttaaaa aacaagaaag aaaaatcgcc
     1921 atatgcgggg aacatgtttg tttatgacaa tctcatcaga attcgtggcg aaattccggc
     1981 ggaatacctg gcaagagtcc atcaggcatt gcttaaaaat ttggaaacag aattatttga
     2041 tggcaacact aacggtttct tcatggtatc aggccttgag aaagactggg atgcagaaaa
     2101 acgctggaat gttgctacat ggttattcag taacagagcc gctgcactgg aagcttcggc
     2161 atgtatttgc ggcctgttct taacagacca caaatataat ctggatgtgt acagttatat
     2221 ttacgctgaa cacggtccac tctggattga ctggtaatta taaggaaaca ccagcagggc
     2281 cgcggcgacc aacagaacga ttaaaatcaa taatgccatt ataaaggaca ttatttaatt
     2341 tatcgtcgaa tgctgattct gtgagcctca actctgaatg agtttttaat aaccctgatt
     2401 gcctgagttg atttaccagg cattcaatct gtttttcaat aagcggattt ctttttttgt
     2461 ttggcatttt atcctccatt gaggttctgg gttaaaaatg gagaccaaca cgctgtcacg
     2521 tgtggtcgtg cgccggacac ggataagaat ccggtactga cagtttactg aaaggatatt
     2581 tccctgaaaa gtcagtgcat aacgcgaaag cgtacggcga agctctttcc cttagaaggc
     2641 ttgtcgttag atttcttcga acgtgcgctt ccggttgtgg cactccgcga aatggcgcgg
     2701 cggtaagtat ggcggggtta ttccttcccc attgaggaca ccgggttgtc aggttgacca
     2761 tacgcttaag tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg
     2821 gcggtatcag ttttactccg tggctgctct gccgcccttt ttaaagtgaa ttttgtgatg
     2881 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga
     2941 ttctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg
     3001 catcacaaaa ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac
     3061 gtctgaaggt tgttttgata ttggtgttct gctcagtaac cgggagttta ctgaagatgc
     3121 cattaacatg aggaaatatg agccttatct gctcaatgat aattccatac tttcccgaat
     3181 tgctcttctt gaacttggta ttttcggaga acgtcaatga cttcagcatt tgcactgatg
     3241 atgacggttt ttcttataac gggtgaatca cagaatgtga ttaccggaat ttatgccagt
     3301 aaagaatcct gcctccaggc aagagacgag caaaaaattt ctggtgaatg cctcccggta
     3361 aaaaaagtat cgctgtacct gaataacgaa acaccggctg gataaccctc cagccatatt
     3421 aacaccatac caacggatta aaaatgccag caatggcagg gatttgttca cccttaaatc
     3481 tgtaatgagg tttatcaatg agcactgata aagaagaatt tgcgctatat tgcgaagcaa
     3541 aaaatgacaa agtcagaaaa cgtctgggaa ttaaaggtgg tttttactgg actacagcaa
     3601 aaaaattatc tgttgccatc tcccgctgca ttaccgcaat ggatgacaac gattatgatg
     3661 aagacgactt taaaaaaccc gttcgcgtcc atttacccgt tgtgaatgac cttccacctg
     3721 aaggcgtgtt tgataccgaa ttctgcaacc gatacgaaaa aggcggggaa gatggcatta
     3781 caatggtatt tatcgcgccc tctccctccg tgcaggagaa accagccagt actgacaata
     3841 ccaacgtcaa cggcgaagac atgacggaga ttgaggagaa tatgctcctg ccggtttctg
     3901 gtcaggagct gcccattcgc tggcttgcgc aacacggcag cgaaaaacca gtaacgcacg
     3961 ttgcacggga agaacttcag gcattacata tagcacgggc ggaagaactg ccggctgtta
     4021 ctgccttggc catttctcac aaaacaaagc tgctcgaccc gctggagatt cgcgaccttc
     4081 acaaactggt acgcgacact gacaaagttt tccctaatcc cggtaattca gacctgggac
     4141 tgataactgc ttttttcgag gcatacctgg acgctgacta cactgatcgg ggtctgctga
     4201 caaaagagtg gatgaaagga aatcgtgttt cgcgtatcac ccgtacggct tccggtgcaa
     4261 atgccggtgg cgggaacaaa accgatcgca atccgaattt agtacacacc ttcgatacgc
     4321 tggatgtgga gattgcagca gccacacttc cgatggattt taatatttat gaaattccgg
     4381 gcagcgttta tcgtcgcgca aaagaaatcg tcctgaaaag agaaagtccg ttcaaagaat
     4441 ggtccgcagc acttcgcgca accccgggta ttctggacta ttcccgcgcc gctatttttg
     4501 cacttatccg gagcgctcac cctgagtttt atcactaccc gggacgcctt caggggtata
     4561 tcaacgccta cttaacggag actgatcacg agaaccccag caaggaaact ctcactgctg
     4621 cacgacatac accggaaaaa gatatcctgg aagaagttaa ccgcgaactg tctgctaagc
     4681 aggaaacaga agaagaaaat gatgaagaaa aaccgcaacc atcttgcgca atggcagaat
     4741 gataatcaga agaaatctgg caaaagaccg ttttacagta ccaaaaacac aggcgggaac
     4801 caatcgtgtg attcacctta ttaagcccgc aatcgacgct ctccggagtc agatggcact
     4861 aacgagactg agcaaagagc atatcatcga tgttcacctc agagaattcg gcagaacaga
     4921 gaaacaaaaa tgtacctttg tttttcaacc tgaagtgtca gcgaaagtaa aaaattatgg
     4981 cgaccatttt accgttgact caataaggca gatgtgggac gcagcggtaa agcgtgccgg
     5041 aatccgccat cgcaaatcgt atcagtcgag acacacttat gcctgctggt cgttaacagc
     5101 aggagctaac ccggcattca ttgcaaacca gatgggccat gcagatgcgc aaatggtgtt
     5161 tcaggtgtac gggaaatgga tgtcagaaaa taataatgcg caggtaacgc tgttaaatac
     5221 acagttaagc gagtttgccc caaccatgcc ccataacgaa gcgatgaaaa gttaattttt
     5281 tatttatcaa ttagttagat t
//
LOCUS       DAZFKY010000246         5244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFKY010000246 DAZFKY010000000
VERSION     DAZFKY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHKCL_004409"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHKCL_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO5765242.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHKCL_004410"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHKCL_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1579 a         1144 c         1183 g         1338 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggtaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 tctccattat ttataaggga ggtg
//
LOCUS       DAZFKY010000247         5221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFKY010000247 DAZFKY010000000
VERSION     DAZFKY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            53..529
                     /locus_tag="ACHKCL_004411"
     CDS             53..529
                     /locus_tag="ACHKCL_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5765243.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            526..2649
                     /locus_tag="ACHKCL_004412"
     CDS             526..2649
                     /locus_tag="ACHKCL_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5765244.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2646..2858
                     /locus_tag="ACHKCL_004413"
     CDS             2646..2858
                     /locus_tag="ACHKCL_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765245.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2858..4360
                     /locus_tag="ACHKCL_004414"
     CDS             2858..4360
                     /locus_tag="ACHKCL_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765246.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4305..>5221
                     /locus_tag="ACHKCL_004415"
     CDS             4305..>5221
                     /locus_tag="ACHKCL_004415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFO5765247.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNE"
BASE COUNT         1219 a         1237 c         1628 g         1137 t
ORIGIN      
        1 ttacaaaaaa acagagtcac ttctgttctg cggtttatgt ggagggatgt aaatggacgg
       61 cgagctgaaa aatatgaagt taaatattaa tcaactggca gccctttcag gtctgcaccg
      121 gcagactgtt gccgccagaa tggcggatgt tcctcttgca ccaggcagta atgaaaagaa
      181 aaaactgtat ctcctgacgg atttgataac ttcgttgctg gaaaaaccac catcttccga
      241 agatgaggat atggatcctc acgctcgtaa ggcatggtat cagtccgagc gcgagcgtct
      301 taaatttcag catgaaactg ttcagcttgt gccagtcagt gatgtcaggc ggtccttttc
      361 tgtcgtggtg aaagcgatag ttcaggtact ggaaacctgg cctgaccggc tggagaggga
      421 cagggggtgg actgcatcac aactgaatga agtacagatt gtggttgatg agatccgcga
      481 cacactggaa aaggcagtca ttgactgttg tgatgaggcc gatatgtgaa tcaggtgaac
      541 gagagccata gccgcgcatc cgatatctgg cgcgaagtgg cctcgctgtt tcgcccaccc
      601 agccggttac cagtagcgga agccatcagg cgttatatgc gggttccacg gggagccaat
      661 acttccggtc cgtgggagtc atcgctgacg ccctatatga ttgaccccat taatacatta
      721 tcagcccgtg aatatgacgc ggtggtgttt gtgggacctg cgcgaaccgg gaaaaccgaa
      781 gggctgattg atggctggat tgtgtacggc atcatctgtg atccggcgga tatgctggtg
      841 gtgcagatga ctgagacgaa ggcgcgtgag cattccagaa cgcgtctttc caggacgttt
      901 cgccacagtc cggaggtcag caagcgcctc agtccttccc gtaatgacaa caacgtccac
      961 gataaaatgt ttcttgacgg ctccttcctg aagattggct ggccgtcgat caccgtcttt
     1021 tcctcttcgg attaccgtcg tgtggcgctg acggattatg accgtttccc tgaaaatgtg
     1081 gacggggaag gggatgcctt cacgctggcc tcaaagcgta ccaccacctt tatgtcctcg
     1141 gggatgaccc tggtcgagag ttcaccgggg cgggatatca ccgataccaa atggcgttgt
     1201 ggtggcgcac atgaggcacc gccaacaacg gggatcctgt cactgtataa ccggggagac
     1261 cgccgccggt ggtactggcc gtgtccgcac tgcggggaat attttcagcc ggtgatggat
     1321 aacatgaccg gataccggaa taacccggat tttgtggctg ccggtcaggc tgcccgtctg
     1381 atgtgtccgc attgtcgcgg gctgattgcc cctgagcaga aacgcgaact gaataaccag
     1441 gggatctggc ttcgtgaagg tgaacgggcg gcggcggacg gcagtatcac cggaacgcca
     1501 cgaaactccc ggattgcgtc attttggatg gaggggccag ctgcggcgtt tcagacctgg
     1561 gaacaactga tttttaaact gctggcggca gaagaagagt atgagcgaac cggcagtgaa
     1621 gagaccctga aagcggtggt gaacaccgat atcggacgac cctatctgcc ccgttcagcc
     1681 acggaacagc gtaaaagtga actgcttgaa cagcgtgccg agccgtttcc ccggcgatct
     1741 gtgccggatg gtgtgcgttt tattgaggca acggttgacg tacagggcgg taaaaatcgc
     1801 cgttttgttg tgcagatcac cggatacgga gagcaggggg aacgctggat tgttgatcgc
     1861 tacaacatcc ggcattcact gcgctgcagt cccaacggtg aaagtctgcc ggttgatccg
     1921 gcggcatatc cggaggactg ggatttgttg ctgacggatg tgttccataa aacatggccg
     1981 ctggcttctg atccggatgt gcgcatgcgt ctgatggcca tggcggtgga tacgggaggg
     2041 gaagccgggg tgacagataa cgcctatcgt ttctggcgtc gttgccggag tgacggactg
     2101 ggcaacaggg tgtttctgtt caagggggat ggacttcgcc gtgacaggct gattaaccga
     2161 accttcccgg ataataccgg cagaagtgcc cgccgtgcca gagccagtgg cgatgtcgcg
     2221 ctgtggctgg ttcagacgga tgcgtttaag gatcgtgtaa ataatgccct gtggcgtgac
     2281 acaccagggc cgaactatat ccactttccc gactggctgg ggcggtggtt ttacgatgag
     2341 ctgacctatg aagagcgcgg cagtgacgga aaatggcgaa aaccgggcag gggcgctaac
     2401 gaagcgtttg acctgctggt ttatgcggat gcgcttgccg ttctgcatgg ttacgaaaag
     2461 atccgctggc cctccgcacc ggactgggca cagcgggaaa cgtggctcgt cttcccgcag
     2521 gagcgttctg gtgaaacggt atccccggaa ctgacggccg gggcagaaaa acgccgtcgc
     2581 cggaagaaaa aactgcggac ggagcgtgcg gaagataatc catggataac atcaggaggc
     2641 tggttgtgag cacagaagaa gccagagaaa tgatacagcg gtaccgtgaa gcggaaatgg
     2701 ccgtactgga gggaaagtct gtcatcttca acgggcagca actgacgctg gaaagccttt
     2761 ctcagatccg cgccggacgt caggagtggg aacgcaggct tgccgcgatg gtgagccgca
     2821 ggcggggaaa accgggattt aaactggcga ggttttaatg gcaattattg atgatgtgat
     2881 cggcgtgttt tcccccgggt ggaaagcagc cagactgcgt tcaagggcgt taatcatggc
     2941 ctatgaggcg gtgaaaccga cccggacaca taaagcccgg cgggaaaatc gctctgctga
     3001 tcagctcagt aaatacggtg cggtttccct gcgggagcag gcccgttttc tggatatcaa
     3061 tcatgacctg gtgattggtg tgtttgacaa gctggaagag cgggtgattg gtgccagggg
     3121 aattattgtg gagcctcagc cattacgaaa aaacggggaa atggcggctg agctggctgc
     3181 ggatatccgc cgtttgtggg ctgaatggtc cgtgagtccg gatgtgacag ggcagtatac
     3241 ccgtcctgtg cttgaacgtt tactgctgcg gacctggctg cgggatggtg aagtgtttgc
     3301 gcagatggtc agtggtgcgg gaaacggtct ggaacggacg gcgggagtgc cattctggct
     3361 tgaggcgatg gagccggatt ttgttcccat gcgcactgat gaatccgccg gactgaatca
     3421 gggggttttt cttgatgagt ggggaagacc gaaaaaatat ctggtttata aaaattatcc
     3481 ggtcagcggc cggcagagtg atacgaaaga aatcgctgcc ggaaaaatga tccacctgaa
     3541 gttcactcgt cgtctgcatc agacgcgagg ctcatccatg ttatcggggg tgctgatgcg
     3601 gatcagtgcc cttaaggagt atgaggatgc ggaactgaca gcggcgcgta ttgctgcggc
     3661 gctgggactg tatatccgta aaggtgacgg acaggactat gaagatccgg ggatcaaaga
     3721 gaccgagcgg gaagtccata tcaccccggg tattatttat gacgatttgc gcaagggcga
     3781 ggatatcggc atggtcaaat ctgaccgtcc caatcccaac cttgaaactt tccgcaacgg
     3841 ccagttgcgt gcagtggcag caggcagtcg tctgagtttt tccagtgcgg cgcgtaacta
     3901 taacggcacc tacagcgccc agcggcagga gctggtcgag tccacggatg gttacctgat
     3961 cctgcaggac tgttttattg gcgcggtaac ccgcccggtg taccggacat ggctgaatat
     4021 ggtggttgcg gcaggtctgc tgaaaattcc ggcggatgtg gagatgaaaa cgctatataa
     4081 cgcgacgtat tccggtccgg tgatgccgtg gatcgacccg gttaaggaag ctgaagcctg
     4141 gagaattcag atccggggtg gtgcagcgac agaatctgac tgggtgcgtg ccggtgggcg
     4201 caatccggat gaggtcaaac gtcgccgcaa ggctgaaatt gatgaaaaca gcagactggg
     4261 gctggtcttt gatactgacc ccgtcaacga caaaggaggc aacagtgccg gaactgaacg
     4321 acagtatcag cgcgacaccg aaagccagca tgaagaataa atcctggttc aggatgcaag
     4381 ctggggggcc gggtgacgcg gatatttata tttatgacga gattggtttc tggggagtta
     4441 ccgcgaagca gtttgtcagc gaactgaatg cactgggtga tatcacccac attaatctcc
     4501 atatcaattc accgggtggc gatgtctttg aaggcatcgc catttttaat gccctgaaaa
     4561 atcagggggc gaccattacc gtgtatgtgg atggcgttgc cgcctcgatg gcatctgtga
     4621 ttgcgatggc cggtgatacg gtcattatgc cggaaaatgc cttcatgatg atccataagc
     4681 catggggatt cagtggcggg gatgctgagg atatgcgcag ttatgccgat ttgctggata
     4741 aagtcgaatc ggtactgttg ccagcctatg cgcagaaaac cggaaaaacc accgatgaaa
     4801 ttgccgccat gctggcggat gaaacctgga tgtccggtgc cgaatgtctg gcacacggat
     4861 ttgctgacca ggtgacaccc gctgttgagg caatggcatg tattcagtca aaacgtacag
     4921 aggaatttaa aaagatgccg gaatccatcc gaaacatgat tactccgcca cgcaacagtg
     4981 ccccgcgtga taccacagtg acaatccctg caccggcggt aacagaacca tcaccggtac
     5041 cggcagtgtc tgatgaggcg accattcgcg cccgcgttat ggctgagcag aaagcccgca
     5101 tgtcaggcat taacgatctg tttgccatgt tcggcggtcg ctatcagacg cttcaggcac
     5161 agtgcgtggc tgatcctgac tgttcgctgg aaatggcccg tgaacgtctg ctgaatgaaa
     5221 t
//
LOCUS       DAZFKY010000248         5214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFKY010000248 DAZFKY010000000
VERSION     DAZFKY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..379)
                     /locus_tag="ACHKCL_004416"
     CDS             complement(<1..379)
                     /locus_tag="ACHKCL_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765248.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHKCL_004417"
     CDS             complement(436..831)
                     /locus_tag="ACHKCL_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5765249.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHKCL_004418"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHKCL_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHKCL_004419"
     CDS             complement(1642..2382)
                     /locus_tag="ACHKCL_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5765250.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHKCL_004420"
     CDS             complement(2389..3351)
                     /locus_tag="ACHKCL_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765251.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHKCL_004421"
     CDS             complement(3374..3799)
                     /locus_tag="ACHKCL_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5765252.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHKCL_004422"
     CDS             complement(3796..4098)
                     /locus_tag="ACHKCL_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5765253.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHKCL_004423"
     CDS             4196..4567
                     /locus_tag="ACHKCL_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765254.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHKCL_004424"
     CDS             4588..4779
                     /locus_tag="ACHKCL_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO5765255.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHKCL_004425"
     CDS             4781..5059
                     /locus_tag="ACHKCL_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5765256.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1255 a         1450 c         1149 g         1360 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg
     5161 tttattaaac taaaaatacc aacccacccc gccccacaga acgcagggaa atac
//
LOCUS       DAZFKY010000249         5186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFKY010000249 DAZFKY010000000
VERSION     DAZFKY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..328
                     /locus_tag="ACHKCL_004426"
     CDS             <1..328
                     /locus_tag="ACHKCL_004426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765257.1"
                     /translation="RKALQAYLFLIFIKMLRQLTFLFPTKFHRGKKLRTQRYRGLQSK
                     EKKKLSLLTTKFSDEAWGDNHKNKVVYRAQDRFHNPLFIVLMVVKKKLDHKKLIDNNS
                     HYHVCI"
     gene            604..2586
                     /gene="chuA"
                     /locus_tag="ACHKCL_004427"
                     /gene_synonym="shuA"
     CDS             604..2586
                     /gene="chuA"
                     /locus_tag="ACHKCL_004427"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO5765258.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVGASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            2635..3663
                     /gene="chuS"
                     /locus_tag="ACHKCL_004428"
     CDS             2635..3663
                     /gene="chuS"
                     /locus_tag="ACHKCL_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO5765259.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(3724..4254)
                     /locus_tag="ACHKCL_004429"
     CDS             complement(3724..4254)
                     /locus_tag="ACHKCL_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5765260.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4410..4976)
                     /gene="slp"
                     /locus_tag="ACHKCL_004430"
     CDS             complement(4410..4976)
                     /gene="slp"
                     /locus_tag="ACHKCL_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5765261.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1399 a         1149 c         1244 g         1394 t
ORIGIN      
        1 acgcaaggcg ttacaagcgt atttattttt gatttttatc aagatgcttc gtcagctaac
       61 gttcttgttt ccgactaaat ttcatcgcgg gaaaaagctg agaacccaga gatatcgagg
      121 cttgcagagc aaagagaaga aaaaattatc actgttaacg acaaaattta gtgatgaggc
      181 atggggggat aaccataaaa acaaggtggt ttacagggcg caggataggt ttcataaccc
      241 cctgtttatt gtgttaatgg tggttaaaaa aaaacttgat cataaaaaat tgatcgataa
      301 taattctcat tatcacgttt gcatttaaca aaacacgacc atatggataa caaaaccata
      361 gcatttgcaa gggataacgc acagctctct tcactcctga tggggcatat cgttttgcct
      421 gacaaagagg gcgtgctttt gcgtaattgc cgagttatca ggcaatttca tgggatataa
      481 acgcttgtaa actcacccgg cgaatattat cgctctgaat tttaaataaa tttatttttc
      541 gccagccacc ccggtattaa cccaggcagc cagcattttt tattttcctc atggagaatc
      601 gctatgtcac gtccgcaatt tacctcgttg cgtttgagtt tattggcctt agctgtttct
      661 gccaccttgc caacgtttgc ttttgctact gaaaccatga ccgttacggc aacggggaat
      721 gcccgtagtt ccttcgaagc gcctatgatg gtcagcgtca tcgacacttc cgctcctgaa
      781 aatcaaacgg ctacttcagc caccgatctg ctgcgtcatg ttcctggaat tactctggat
      841 ggtaccggac gaaccaacgg tcaggatgta aatatgcgtg gctatgatca tcgcggcgtg
      901 ctggttcttg tcgatggtgt tcgtcaggga acggataccg gacacctgaa tggcactttt
      961 ctcgatccgg cgctgatcaa gcgtgttgag attgttcgtg gaccttcagc attactgtat
     1021 ggcagtggcg cgctgggtgg agtgatctcc tacgatacgg tcgatgcaaa agatttattg
     1081 caggaaggac aaagcagtgg ttttcgtgtc tttggtactg gcggcacggg ggaccatagc
     1141 ctgggattag gcgcgagcgc gtttgggcga actgaaaatc tggatggtat tgtggcctgg
     1201 tccagtcgcg atcggggtga tttacgccag agcaatggtg aaaccgcgcc gaatgacgag
     1261 tccattaata acatgctggc gaaagggacc tggcaaattg attcagccca gtctctgagc
     1321 ggtttagtgc gttactacaa caacgacgcg cgtgaaccaa aaaatccgca gaccgttggg
     1381 gcttctgaaa gcagcaaccc gatggttgat cgttcaacaa ttcaacgcga tgcgcagctt
     1441 tcttataaac tcgccccgca gggcaacgac tggttaaatg cagatgcaaa aatttattgg
     1501 tcggaagtcc gtattaatgc gcaaaacacg gggagttccg gcgagtatcg tgaacagata
     1561 acaaaaggag ccaggctgga gaaccgttcc actctctttg ccgacagttt cgcttctcac
     1621 ttactgacat atggcggtga gtattatcgt caggaacaac atccgggcgg cgcgacgacg
     1681 ggcttcccgc aagcaaaaat cgattttagc tccggctggc tacaggatga gatcacctta
     1741 cgcgatctgc cgattaccct gcttggcgga acccgctatg acagttatcg cggtagcagt
     1801 gacggttaca aagatgttga tgccgacaaa tggtcatctc gtgcggggat gactatcaat
     1861 ccgactaact ggctgatgtt atttggctca tatgcccagg cattccgcgc cccgacgatg
     1921 ggcgaaatgt ataacgattc taagcacttc tcgattggtc gcttctatac caactattgg
     1981 gtgccaaacc cgaacttacg tccggaaact aacgaaactc aggagtacgg ttttgggctg
     2041 cgttttgatg acctgatgtt gtccaatgat gctctggaat ttaaagccag ctactttgat
     2101 accaaagcga aggattacat ctccacgacc gtcgatttcg cggcggcgac gactatgtcg
     2161 tataacgtcc cgaacgccaa aatctggggc tgggatgtga tgacgaaata taccactgat
     2221 ctgtttagcc ttgatgtggc ctataaccgt acccgcggca aagacaccga taccggcgaa
     2281 tacatctcca gcattaaccc ggatactgtt accagcactc tgaatattcc gatcgctcac
     2341 agtggcttct ctgttgggtg ggttggtacg tttgccgatc gctcaacaca tatcagcagc
     2401 agttacagca aacaaccagg ctatggcgtg aatgatttct acgtcagtta tcaaggacaa
     2461 caggcgctca aaggtatgac cactactttg gtgttgggta acgctttcga caaagagtac
     2521 tggtcgccgc aaggcatccc acaggatggt cgtaacggaa aaattttcgt gagttatcaa
     2581 tggtaatcat ctgccccgat atttcggggc atttatctgg aaggaagaga gacaatgaac
     2641 cactacacac gctggcttga gttaaaagaa caaaatcccg gaaagtacgc gcgtgacatc
     2701 gcagggttaa tgaatattag agaagcagaa ctggcatttg cacgcgtcac gcacgatgcg
     2761 tggcggatgc acggcgatat ccgtgaaatt ctggcggcgc tcgaaagtgt tggcgaaacc
     2821 aaatgtattt gtcgtaatga atatgcagtc catgagcaag ttggtacgtt cacaaaccag
     2881 catttgaacg gacatgccgg attgatcctc aatccgcgcg cgctggattt acgtctgttt
     2941 ctcaatcaat gggccagtgt tttccacatc aaagaaaaca cggctcgtgg cgaacgccag
     3001 agtattcagt tctttgatca tcagggcgat gcattactaa aagtttatgc caccgacaat
     3061 accgatatgg cggcatggag tgagcttctg gcacggttta tcaccgatga gaatacgccg
     3121 cttgagttaa aagccgttga tgcgccagtt gttcaaacgc gagccgatgc cactgtggtc
     3181 gagcaagagt ggcgggcgat gaccgacgtt catcagtttt ttacgttgct caagcgccac
     3241 aacctgacgc gccaacaggc gttcaatctg gtggcagacg atttggcctg caaagtatcc
     3301 aacagtgcgt tggcgcaaat tcttgaatct gcacagcagg atggtaatga aatcatggtg
     3361 tttgttggca accgtggctg cgtacagatt ttcaccggtg tggtagaaaa agtggtgcca
     3421 atgaaaggtt ggctgaatat tttcaacccg acgtttactc ttcatctatt agaagagagc
     3481 attgctgaag cctgggttac ccgtaaaccg accagcgatg gctacgtaac cagtctggaa
     3541 ttgtttgccc atgatggtac gcagatagcg caactttatg gtcaacgtac agaaggcgaa
     3601 caggagcaag cgcaatggcg taagcaaatt gcttcgctga taccggaagg cgttgctgca
     3661 taacgtataa ttaacgggag gtctggctat ttattgctgg gcctctttat attgatcaat
     3721 cgttcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc gcttaagatt
     3781 gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata ttttcagttt
     3841 ctccagaata tcttccgttg tcatttgatt cgcaatgcag ctcagaacct gatgttgctt
     3901 gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga tgagtgcgac
     3961 aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggcactttcc ccaggcagcg
     4021 tttaatatta aagggggaga aaataatgac atggacaggt tttaatcgtt caatttgagt
     4081 gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga aggcattctg
     4141 atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac ctttactcag
     4201 aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa acataaccga
     4261 ctcctggtgc ggacattaac tttataatca gtttgccaaa ggtaattaac aggacagggc
     4321 ttataataat aatagttagc ttactgtgta ttaatttgat gtaatgcatg atgcagatgc
     4381 tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt tacctgactc
     4441 accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc accatagccg
     4501 taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac ctggatgccc
     4561 tgcatattca cttccaggaa gttatacggg actttattga taaagccagg ttgttcaccc
     4621 tgaatggtgc cgaggatggt aacaaagtga ttacgatagt tcactggatc aaggaagccg
     4681 ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggctt cgcatagcta
     4741 tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat aacgttgata
     4801 accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt atgaacagca
     4861 acaaaacttt tttgaatatc aggttggtta tttcctttga tattttgcgg aattgaacta
     4921 catgctgcaa gcaaaaatga aaggctgagt atgagtgcac cttttgtcat gttcatgtta
     4981 ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt ttcatctgat
     5041 tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc ccttctgttt
     5101 agttcaattc cgttattttg gggcgtggca tgagtatcca tcgccccata atataaccct
     5161 ttgttttaaa attacataaa atctat
//
LOCUS       DAZFKY010000250         5146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFKY010000250 DAZFKY010000000
VERSION     DAZFKY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(31..1533)
                     /locus_tag="ACHKCL_004431"
     CDS             complement(31..1533)
                     /locus_tag="ACHKCL_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5765262.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(1663..2682)
                     /gene="ahr"
                     /locus_tag="ACHKCL_004432"
     CDS             complement(1663..2682)
                     /gene="ahr"
                     /locus_tag="ACHKCL_004432"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5765263.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2878..2962
                     /locus_tag="ACHKCL_004433"
     tRNA            2878..2962
                     /locus_tag="ACHKCL_004433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2912..2914,aa:Leu,seq:caa)
     gene            3053..5002
                     /locus_tag="ACHKCL_004434"
     CDS             3053..5002
                     /locus_tag="ACHKCL_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFO5765264.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVRDALRRKRGKQTG"
BASE COUNT         1269 a         1339 c         1255 g         1283 t
ORIGIN      
        1 cgcatcaggc aaagacaaat ctcgcctgac ttaccttctt ctccccccca gcaaactccc
       61 caacatcccg cgtacaatct gattcgtcac ttggcgagcg gcgcttttgg ccattgtttg
      121 caccacacca tctttcttcc cgccgcgtgg tccggtagtg ccgaacaaaa tatccttcaa
      181 tccaccaaga atgccgtcat ccaccgctac ctctttccct ttcgcggggg gattattttg
      241 ctgctcggta ctggcctgaa agcctttttg caacatctca taggcggatt cccggtccac
      301 ctcatcctca tatttgccat acaccggaga gtgattaatc aagccattac gctcatcttc
      361 cgtcaccggc cccatccgcg aacaaggcgc gatcaccatc gcacgctcca ccacagaagg
      421 gcttcctttc gcatccagaa aagagatcaa cgcctcgccg gtgcccagtt cctgaatcgc
      481 cttttcggta tcaaacgccg gattggcccg catggtttgc gccgcagctt ttactgcttt
      541 ctgatctttg ggcgtaaaag cccgcaaagc gtgctgaacg cgattaccga gctggccgag
      601 cacattatcc ggaatatcag acgggttttg cgaaacgaac cagacgccca cgccttttga
      661 gcgaataagc cttatcacct gctcaatctt atccagcagt acctgcggtg catcgttaaa
      721 cagcagatgt gcttcgtcga agaaaaacac cagttttggt ttctccagat cgcccgcttc
      781 cggcaattgt tcatacaact ctgagagcat ccacagcagg ctggcggcgt acagtttcgg
      841 catctgataa agtttctcgg cgctgaggat attgataacg cctttaccgt tggcatcggt
      901 gcgcatccag tctttgatat ccagcatcgg ctcaccaaag aagtgtgctg cgccttgctg
      961 ttccagcgac aacagcccgc gctggatggc accaaccgat gcgctactga tatttccgta
     1021 ctgattctgg aaagatttgg cgttatcgcc gatgtactgg gtaatcgccc gcagatcttt
     1081 aaagtcgagc agtaacagcc cctgatcgtc agcaatgcgg aagatgatat tcagcacgcc
     1141 agattgcaca tcgttgagat tcaacagccg cgccagcaac agtggcccca ggtctgaaac
     1201 cgtcgcccgt accgaatggc ctttctcgcc aaagatatcc cacaccacca ccggattagc
     1261 atgcggttgc cagtcattga cgtcgatatt tttaagcctt gcgagcagtt tttccgacgc
     1321 cgttcctgcc tgcgcgatac cggtcagatc gcctttcaca tcagccataa acaccggcac
     1381 gccgatttcc gacaatgact ccgccagttt ttgcagcgtg acagttttac ccgtccccgt
     1441 tgcgccagta atcagcccgt gacggttagc cattcccggc agtaaaaata gttctgtgtc
     1501 cggcgtgcgg gcaattaaca ggggttcgct catgagatat cctccagttt cctgcctgga
     1561 gtataggcaa cggctgcgca ctcttcaccg ctaaattcag cagattgcga gacgccttcc
     1621 aaagagattt aagtgccggg cgacaggcac ctggcaaaga gatcagaagt cagctttcag
     1681 aaccacgcgg taacgcgcct taccgtcgcg cacatgcttg atggcatcgt taattttcga
     1741 catcgggaac aattcggtag tcggcgcaac cttgctgcgg gcggcaaagc gcatcagctt
     1801 acgcaactca taaggtgtac cggtagcaga gccagagaca ctgcgatcgc ccgcaatcag
     1861 cgtaaaggcc ggaaccggca acggcgtgag aaccgcaccg accgtatgga aattaccgcc
     1921 ataggtcagc gcctcaaaat aaggctgcca gtcgaggctg acgttcacag tattgataat
     1981 gagatcaaac tgccccgaca gggctttcag tgcctgcgga tcgcggctat tcaccacttt
     2041 atcggcaccc atcgccagta cttcctgctc tttcgccgga ttagaactaa aggccgtcac
     2101 ctcgcatccc attgcgtgca gaagttttat agcgatatgc cccagcccgc caataccaat
     2161 taccccaacg cggctggtag cagtgatatg gtgcatcaac agtggtttaa agaccgtgat
     2221 accgccgcac aacagcggcc cggcggactc gatatcaata ttttctggca gtggaatcac
     2281 ccattgccag tccgcacgca acttctcggc aaagccaccg cgattcataa tcgtcggcac
     2341 agcaccttgc tcgcagttga tctgattacc gctaatacag gcgtcgcagt gaccacagct
     2401 acgcgccgtc cagccaatcc cgacacgctg gccgacctgc aaacctttat cctgcgcggc
     2461 gctcccgagc gccaccacgc gcccaatcac ctcatgcccg gcaaccagcg gatattgtga
     2521 aaatccccat tcattgtcga tcatcgatag atcggaatgg cagatcccgc aataatccac
     2581 ctgcacttca acatcttgtg gcctcagctc accgggatcg tactcataaa cttccagttc
     2641 gccgcccgct tcttttgcgg catagctttt tatcatcgac attttttggt ccttctctgg
     2701 tgttgtttgg gaagtgtaga gcatggcagg gcggggtgcc tggagtgtga caaaggttac
     2761 acatcgctgt atgcaatgct gaaaatttca gcacttagcg aggtgcgagc aagctggcgc
     2821 ttgcatggtg gcgtgcgaca ggtataatcc acaacgtttt ccgcatacct cttcagtgcc
     2881 gaagtggcga aatcggtaga cgcagttgat tcaaaatcaa ccgtagaaat acgtgccggt
     2941 tcgagtccgg ccttcggcac caagactcat ctaaactatt gttttaaaac aatttaattc
     3001 ataatcttgc ttgaaatcgc gtagcccctc ttacaaaagg gtatacgagg cgatgtctca
     3061 gcgatataaa ctctatcgtc gaaccagcgg catttatgtt gtccgcatta gcgttcccca
     3121 acgttttcgt cgatacgcag gacagtgtga gattcacact tcaacaggca ctcacgatct
     3181 tcatgaagca aagcagaagt ccgcgctcct gttggcggtc tggtatcaga ccttacaaga
     3241 gtatgaacaa ttggattatc gaactttaag tgactgcgcc ccactgcttg ctggtgaggg
     3301 tatgatctcg ctttctaact ttgctcaatc aatcgagttg cctatatcgc aattgattcg
     3361 agaggtggtt aatcgtaacc tcccggtatt ctggctggcg actggtcagt tcggtttcta
     3421 tgttgatgaa tttaatgcag tagagcggga accgggtgca aaacgagaaa aacagtctga
     3481 tgatgaaaag gatcaaccta aagaagtcat cattctcaat agcgcgtttg agctgggtat
     3541 cgagagcttc gcaaatggtt atctccgccc cttcaatccc cggcatactt tagattgtct
     3601 gttgagcgct ggagtatccg aaggagaggc tgcatttcga actagtggtg ataaccaaag
     3661 tggaggttgg ttcttcgatt tacccggcgt agatataact gctgatagcc tgttgattag
     3721 caaagttcat gctgaaggcc ttcgacttac atggctggtt aagaccacgc caccagcagt
     3781 tagcattcac cctgccgtgc ctcttgtcgc tcctgttatc gctaatgaat atgttcaccg
     3841 caaacattac aatgaaaact tgtcatggct tcgtgaagag tatttgaaac atcgacgtaa
     3901 gggcaaggta tcagaagcgg cgctccgcga tattcgctat tacttcgatt tgatgattga
     3961 agtgatgggg gatattcagt tggaagattt cgaccgtgat ttcctccggg cttatgagag
     4021 caagttgcgc acaattcctg ctaaccgtaa tttgatgaaa ggtaagcacg gggttaagac
     4081 gctggatgag ttaatcgcca aagcggcaga atgtggcgat aaactgatga cagaagagtc
     4141 tgtcaaaaag tatatcaacg gcctttatgg tgcaatggag tgggctgttg atgatggtaa
     4201 gtttctgaaa tcgccatgcg acaacttttt ccctcccgat gacaaaggtg agcgagagca
     4261 ggatcacact gacatatttg aaccgcatga aattaaggca attttttcgc aaccgtggtt
     4321 tgtcgctgga actgttgaac gtaatgcgca agggcgattc catcaatatt gcccgtttca
     4381 ctattgggcg ccgttgttgg gcttgatgac gggggcaagg gttaacgaga ttgcacagtt
     4441 aatgctggac gatgttctgg cagatgacgg cgtttattac ctgaaccttg aaagcgatag
     4501 cgaaaacgga aagaaactaa aaaacgccaa ttcccgccgc aagattccgg ttcattctac
     4561 gctgattgaa ctcggtttta tcgagtatgt ggatgcgttg aaagctgccg ggtatgaccg
     4621 tctttttccc gagcttaaac cacataaaac caaaggctat ggtaggccgg tttccgcatg
     4681 gttcaatgaa tcattgcttg cgggtcgatt aaaacttgaa agagacagaa gcaaatcttt
     4741 ccactctttc cggcattctg tttcaacttt gcttaaagag aagggtgtta gttcggaact
     4801 gcgtgggcag ctacttgggc atgtgcgagg caaaacagaa actgaagtgc gatacagcaa
     4861 agatttaaaa ccggttcaca tggttgaggt tgtcgaaaag attgattttt ctttgcccga
     4921 gatagcgaga ttcaacattc ctgatgggct ggatgctgta agagatgcgc tgcgaagaaa
     4981 gcgtggcaaa caaacaggtt gagtcagtta ctattcagtt gagttatgct cttgaatgcg
     5041 ctgggacgct tcctggcgca ttttttatta aaggtcgtgg atttaatagt gccaaatttg
     5101 gagacgctta aaatgcgtct ggtgagactt gatttgcatt agctca
//
LOCUS       DAZFKY010000251         5138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFKY010000251 DAZFKY010000000
VERSION     DAZFKY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..200)
                     /locus_tag="ACHKCL_004435"
     CDS             complement(<1..200)
                     /locus_tag="ACHKCL_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5765265.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAF"
     gene            complement(276..419)
                     /gene="kil"
                     /locus_tag="ACHKCL_004436"
     CDS             complement(276..419)
                     /gene="kil"
                     /locus_tag="ACHKCL_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5765266.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKRRSSK
                     SIH"
     gene            complement(388..552)
                     /locus_tag="ACHKCL_004437"
     CDS             complement(388..552)
                     /locus_tag="ACHKCL_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5765267.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDIPNQPGVPK
                     NGSNTYGYPD"
     gene            complement(625..993)
                     /locus_tag="ACHKCL_004438"
     CDS             complement(625..993)
                     /locus_tag="ACHKCL_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO5765268.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPSMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            1254..1835
                     /locus_tag="ACHKCL_004439"
     CDS             1254..1835
                     /locus_tag="ACHKCL_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO5765269.1"
                     /translation="MNNSWWQELMHFFLQGMTLKQLIHMLIILIILIIVMPVSVKEWI
                     NLHNPEILPHYWMYYILLFCVSYVLNGVVNSAYHAVTERIEIFAAQKRKSKEEKYVQD
                     LFDSLTLGERAYLAFAVAANNQLKTEKGSPEAISLLEKGLLIRVPSATGYPEIDRFVI
                     PERYRNECYIRFAGKKDSLMDELIAQDKHGKNK"
     gene            complement(1852..2124)
                     /locus_tag="ACHKCL_004440"
     CDS             complement(1852..2124)
                     /locus_tag="ACHKCL_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765270.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2637..3188)
                     /locus_tag="ACHKCL_004441"
     CDS             complement(2637..3188)
                     /locus_tag="ACHKCL_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765271.1"
                     /translation="MNAAQSSLDEGKLVIAYSDKNGSTVGLEFSSVASSQATLLMKAC
                     SVAASDKEKRIVTSVVMDDTEIIQTTSDDGGDDMDKRLAVLEAEVAHIKSSMAGIKED
                     TRKISSDSTDAKRDTAVLLQKSLDFDASLSKKPSVDYFEAKFSALETKIADVKVWMLG
                     VLLASLAMPTIFFLINLYLKKGQ"
     gene            complement(3195..3476)
                     /locus_tag="ACHKCL_004442"
     CDS             complement(3195..3476)
                     /locus_tag="ACHKCL_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765272.1"
                     /translation="MSDKQEVSLAHINDVLESKNYIETNADGVVTIGTDNNGYEVFNF
                     VFLNSTPVIGHMNGEIAVAGMQRTKVASVTLSKQKAFDFYQSLKSMFEE"
     gene            complement(3599..4246)
                     /locus_tag="ACHKCL_004443"
     CDS             complement(3599..4246)
                     /locus_tag="ACHKCL_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5765273.1"
                     /translation="MKTMHEIIGERIKSLREAKGLSQAQLAKLCGWAAPSRLGNYELG
                     TRKVSADDALVLGAALGVSPAKIMFGEDSDAVFRQYEYPLFSSVQAGPFSEVGSYTAS
                     DAKAWVPTTTKASEKAFWLEVKGHSMTAPQGVRPSFPEGMLILVDPAEPVESGDFCVA
                     SANGDSEATFKKYEKDAGVSYLVPLNPAYRTLDCDHSCRIIGKVVKAQWPEETFG"
     gene            4355..4573
                     /locus_tag="ACHKCL_004444"
     CDS             4355..4573
                     /locus_tag="ACHKCL_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765274.1"
                     /translation="MNKIAQQRKKIGVSQAVLASAIGWGQSRIANYELNIRTPGLNDC
                     RMIVEGLRKLGCQCSLDDVFPPSSNKAA"
     gene            4688..4984
                     /locus_tag="ACHKCL_004445"
     CDS             4688..4984
                     /locus_tag="ACHKCL_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5765275.1"
                     /translation="MAQASYSKPTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            5017..>5138
                     /locus_tag="ACHKCL_004446"
     CDS             5017..>5138
                     /locus_tag="ACHKCL_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5765276.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYS"
BASE COUNT         1376 a         1164 c         1132 g         1466 t
ORIGIN      
        1 tcaaacgcac gggtaatggc ttttttgctg gccccgtggc gttgcaaatg atcgatgcat
       61 agcgattcaa acaggtgctg gggcaggcct ttttccatgt cgtctgccag ttctgcctct
      121 ttctcttcac gggcgatctg ctggtagtaa cgcgcccagc tctgagcctc aagacaatcc
      181 tgaatgtaat aagcgttcat ggctgaactc ctgaaaatgg ctgtgaaaat atcgcccgcg
      241 aaatgccagg ctgattagga aaacaggaaa tggggttagt gaatgctttt gcttgatctc
      301 cgtttcagca ttaatatcca ttttttataa gcgtcgacag cttcacgaaa catcttttca
      361 tcgccaataa aagtggcgat agtgaattta gtctggatag ccataagtgt ttgatccatt
      421 ctttgggact cctggctgat taggtatgtc gataaggcgt ttccatccgt cacgtaattt
      481 acgggtgatt cgttcaagta aagattcgga agggcagcca gcaacaggcc accctgcaat
      541 ggcatattgc atggtgtgct ccttatttat acataacgaa aaacgcctcg agtgaagcgt
      601 tattggtatg cggtaaagcc gcactcaggc ggccttgata gtcatatcat ctgaatcaaa
      661 tattcctgat gtatcgatat cggtaattct tattccttcg ctaccatcca ttgaaggcca
      721 tccttcctga ccatttccat cattccagtc gaactcacac acaacaccat atgcatttaa
      781 gtcgcttgaa attgctataa gcagagcatg ttgcgccagc atgattaata cagcatttaa
      841 tacagagccg tgtttattga gtcggtattc agagtctgac cagaaattat taatctggtg
      901 aagtttttcc tctgtcatta cgtcatggtc gatttcaatt tctattgatg ctttccagtc
      961 gtaatcaatg atgtattttt tgatgtttga catctgttca tatcctcaca gataaaaaat
     1021 cgccctcaca ttggagggca aagaagattt ccaataatca gaacaagtcg gctcctgttt
     1081 agttacgagc gacattgctc agtgtattca ctcgttggaa tgaatacaca gtgcagtgtt
     1141 tattagtatg cctgtctttt aaccacatca ggctcggtgg ttctcgtgta cccctacagc
     1201 gagaaatcgg ataaactcta ttcaccccta cagagagcaa aagagaaacg ccgatgaaca
     1261 actcatggtg gcaggaacta atgcattttt tcctgcaagg aatgacactt aaacagttga
     1321 ttcatatgct aatcatcctg attatattga ttattgttat gcctgtaagc gtaaaagaat
     1381 ggataaacct gcataatcca gaaatccttc ctcattactg gatgtattac atcctgttgt
     1441 tttgcgttag ctatgtgctt aacggcgttg ttaattccgc ttatcacgct gtgactgaaa
     1501 gaattgagat attcgctgct cagaagcgca aatctaaaga agagaaatac gtgcaagatt
     1561 tgtttgattc gttaactctt ggagaaagag cgtatttggc attcgctgta gccgctaata
     1621 accagctaaa gacagaaaag ggaagccctg aagcaatctc attgctcgaa aaagggcttc
     1681 ttattcgggt accttctgct actggatatc ctgaaatcga ccgttttgtt atcccggaac
     1741 gctatagaaa tgagtgctac attaggtttg ctgggaagaa agacagtctt atggatgaac
     1801 ttatcgctca ggataagcat ggcaaaaaca agtaattagc aaattaattt atcatctcgc
     1861 cgtcagttgt tttgatttcc ggtagcctgc cgcgtaaatg gctacgtttg gcaggcaaat
     1921 acttccactg cattcatctg ccttcttgca gcgaaggctt ccgagtgatg ctgctttgtc
     1981 tgctctgacg caaccagaga gctttagcgc aatttttcgc gccagtgctt cattactgcg
     2041 tcgctcggca ataagttctg ctctgcgagc tttgtagcgg ctttttgccg tacctttgga
     2101 ttctttccag acaatggtta ccatgatggt ctcctttaag tggctttggc gcatgacgcg
     2161 tcgaggtgtt tatcttctcg atcgctgtct tgcagctgca attcgcgcca tccccaaaac
     2221 cactcaagtt ctggtctcaa cggttaggtt gagagttcgt cgatgttaaa gagcctgcca
     2281 atctgttcct tgtggcgtct cagcgtcctg ctgatggaaa tgatagtcac atattgtgat
     2341 ttaatggtca atcacaaaat gtgtaaaata aaggtgtgac acgttatgtg tatgattttt
     2401 ttgtgtaaat agttttaccc gcgatggttt tgcttactta agtcggtggc aacagcagga
     2461 tgggtgctga aaagttcgaa aaacgagcga atggggtggt ggcgagatgg cgggatatgg
     2521 gagttgtatt aagaattata gtaatttaat caatagtttg gaccggcggg caggtgctgt
     2581 cctgatagtg atcggcgggc aataaaaaac ccggctcggt ggccggattt gctaaattac
     2641 tgacctttct taaggtacaa gtttattagg aaaaatatag ttggcatagc aagcgaggcc
     2701 aagagaaccc cgagcatcca tacttttaca tctgctatct tggtttccaa agcggaaaat
     2761 ttagcttcaa agtagtcaac cgatggtttc ttagataatg aagcatcaaa atccaggctc
     2821 ttctgtaaaa gtacagcggt gtctcttttg gcgtcagtag aatcagaaga tattttccgg
     2881 gtatcctctt taattcctgc catgctgctc ttgatgtgcg caacttcagc ttcaagaact
     2941 gctaatcgct tatccatgtc gtcgcctcca tcatcacttg ttgtttgaat gatctcagta
     3001 tcatccatca caaccgatgt gacaatccgt ttttctttat ctgaagcagc aacagaacaa
     3061 gctttcatca agagcgttgc ttggcttgat gctacagaag aaaattccag ccctactgta
     3121 gagccattct tatcagagta agcaattact agctttccct catccaaaga tgattgagct
     3181 gcattcatca gcttttattc ctcgaacatg cttttcaagg actgatagaa atcgaaggcc
     3241 ttctgtttac ttaaagtcac ggacgcaacc ttagtgcgct gcattcctgc cactgcgatt
     3301 tcgccattca tatggccgat gacaggggtg ctattgagaa aaacgaagtt aaaaacttcg
     3361 tagccattgt tatccgttcc gatggttaca accccatcag cattagtttc aatataattt
     3421 ttggactcca gcacatcatt gatatgtgct aaggaaactt cttgtttatc gctcatttat
     3481 gttctcctga accaacaaaa taggtaaaaa tcttttcaat ttcattagat taatttgcat
     3541 aaaaagaaca gggaaaagtt ggattaccag ctatgcgccg accagaacac cttgccgatc
     3601 agccaaacgt ctcttcaggc cactgcgcct taactacctt gcctatgatg cggcagctat
     3661 ggtcgcaatc cagggttcta tatgccgggt ttaaaggtac caggtaacta acccctgcat
     3721 ccttctcata cttcttgaac gttgcctctg aatcaccatt tgcagaagcc acgcagaaat
     3781 ccccagactc taccggctca gccggatcaa cgagtatcag catgccttcc ggaaaacttg
     3841 gacgaacccc ctgaggcgcc gtcattgaat gccccttcac ctcaagccag aaagcttttt
     3901 cgctggcttt tgtggtcgtt gggacccatg cctttgcatc gctggctgtg tagcttccca
     3961 cctccgaaaa tggcccggcc tgcactgaag aaaataacgg gtattcatat tggcgaaata
     4021 cggcgtctga atcctcgcca aacattattt ttgccggaga tacgccgagt gccgccccaa
     4081 gaaccagcgc gtcatccgcg ctaacctttc ttgttcctaa ctcgtagttc cccaggcgtg
     4141 aaggcgcagc ccagccgcaa agcttggcca attgagcctg gctaagtcct ttagcttctc
     4201 taagggactt gatcctttcc ccgataattt catgcatcgt tttcatccct taaatgtaac
     4261 acgcaacgtg attgaactct gtacacgaat tgaggttgac tgttaatcac aaattgtgtg
     4321 taatgggtgt gtgattaatg ctagggagac cgcaatgaac aaaattgccc agcagcgaaa
     4381 aaaaatcgga gtttcgcaag ctgtactagc ttcggcaatt ggttgggggc aatcccgcat
     4441 cgccaactat gagctgaata tccgtactcc tggccttaac gattgccgaa tgatcgtaga
     4501 aggcctcagg aagttagggt gccaatgttc tttggatgat gttttccctc catccagtaa
     4561 caaagccgcc taagcagtac ccgctctttc atcatggcct ttcacctcta accgggtgag
     4621 caaacatcag cggcaaatcc attgggtgtg ccgctataac tcaatatcaa tataggaaaa
     4681 ttaacaaatg gcacaagcaa gctacagcaa gccaacacag cgagaaattg atcgcgctga
     4741 aactgattta ctcatcaacc tgtcaacgct tacccagcgc ggtctggcaa agatgattgg
     4801 ctgtcatgaa tcgaagataa gcagaacgga ctggcggttt attgcttcgg tcttgtgtgc
     4861 tttcggaatg gcatcagaca tcagtccgat tagcagggct tttaagtatg cattggatgg
     4921 aatcacaaag aaaaaatccc cggtggccgc cggggactct aagcaaattg atatgcaatt
     4981 ctgagggaat tactggatca atccacagga ttcattatga caaatacagc aaaaatactc
     5041 aacttcggca gaggtaactt tgccgaacag gagcgtaatg tggcagatct cgatgatggt
     5101 tacgccagac tatcaaatat gctgattgag gcttattc
//
LOCUS       DAZFKY010000252         5118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFKY010000252 DAZFKY010000000
VERSION     DAZFKY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..2384)
                     /locus_tag="ACHKCL_004447"
     CDS             complement(<1..2384)
                     /locus_tag="ACHKCL_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5765277.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
     gene            complement(2480..2668)
                     /locus_tag="ACHKCL_004448"
     CDS             complement(2480..2668)
                     /locus_tag="ACHKCL_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5765278.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHKCL_004449"
     CDS             complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHKCL_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5765279.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(3334..3486)
                     /locus_tag="ACHKCL_004450"
     CDS             complement(3334..3486)
                     /locus_tag="ACHKCL_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5765280.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(3761..4405)
                     /locus_tag="ACHKCL_004451"
     CDS             complement(3761..4405)
                     /locus_tag="ACHKCL_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5765281.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            4503..4730
                     /locus_tag="ACHKCL_004452"
     CDS             4503..4730
                     /locus_tag="ACHKCL_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5765282.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            4727..>5118
                     /locus_tag="ACHKCL_004453"
     CDS             4727..>5118
                     /locus_tag="ACHKCL_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5765283.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSG"
BASE COUNT         1212 a         1223 c         1192 g         1491 t
ORIGIN      
        1 cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag tcccagagca
       61 accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat cccctgccgt
      121 tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccggaatt gcagcagtgc atcatccaac
      241 gtgatttcat cggtcagaat ggaggattgt gcttcgcgtg actgtttcag ccaccactta
      301 atggtgtccc gatcgatgac cccacctgcg gtttccagat cgatagtttt gctgaattct
      361 ggccccatct ctccggttgc cggatcaaaa aacttaccgg ctatagagtt tattggcgca
      421 tcaggatttt ttcccattgt ttcaaggtca atcatcagat ggtgccacaa cctgctggtg
      481 gatgagattt catgatgacc gttcacttta attaagggat ttgctgtttc gccagtttta
      541 ttatcgctgg cgtgatactg atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct
      601 tccatttcct ccggatcatt tttctgagct tcagcctgat tctcttcatc gaatgtttcc
      661 aggtaggttg cgttccccat caccgcacca cagtcaggac agttgccgcc accggtctga
      721 ccgcatgcgg tgcaaacttt ttccagttcc tgttgcactg ctggttctgg ttgttgttct
      781 tctggcccgt tttgttgcgt atccgggctg ttttgttccg cttctggctc attttgtttc
      841 acgttgggct gattctggtc ctcagcgtcg cgactctgga tccctttcac ccatttcgga
      901 tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg atgcagcaag caatttatcg
      961 gcgtcaggct ggctgatatt ggctgcctgc ataattttgt ttacttcgtc agcggtaact
     1021 tttaccggct ctgattgttc ggaatcttca gcggtatcca cattttgcgg taagcccgtg
     1081 tatgtgccat tttttcgggc aaaatattct tcttttgaga tttcggtacc gccggcagcc
     1141 agcgccttgt ccagaccaga aagtttgttt gccctgccgt atttttcccc gcccttatcg
     1201 gtaaagacga aataaaatgg tccttcacgc tctacagatg gttcagcttc caccaggatt
     1261 tcattttttt gagtatcgga ttctgccgtc tccactggag cagtttgtgc tgctgacggc
     1321 tggagagcat cagtagagct ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc
     1381 aggtattcac tgatatattt gttcagtgca acgggatctt tgtgaatatc gatcggacgt
     1441 tcgcgaacaa ggccaaaaat agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc
     1501 attgatgctg agatacgctt ccagtcttcg cgatccttgt cgataacctc atttgtcgcc
     1561 cagcgatgga tggtaccgtc aatgtttcct gcatccacat caccgggcca gagggcgcag
     1621 gccagttcat tgtcgagtgt tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg
     1681 acagccccgg ttttttcagc agcgttttca gtgttctgtt ggttgactct ggcgcgggtg
     1741 agatcaacaa cagacgtgta ttttccggtt tccttgcgtt caccttcgcg acgttttttc
     1801 cagatgcgca tctctgcctg aatttcaggc catttagcgc caggctcaca tttatgctta
     1861 acccacccga tggcatgcag tttaagttca ggatacatgg cgttaacttc tggcattttc
     1921 atcaacgctt caacgatatg tccgccgaat gttgccatgt cttcctgtag taattcctgt
     1981 gcgctaatca ccatatcaac ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg
     2041 ttctgtactt caggggacag cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg
     2101 accggtacaa aggaagctga ctcctcatcc cagcggtttt cctgcatata ttcggtatcc
     2161 caggaatcga gggcagggcg gggtatgccg ggtttatcct cgcagacaat aaatttataa
     2221 gcgcagtcct gagctgccgg gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg
     2281 cgacgctcat caccggcttc aatggcagtg gccaccgcaa cagcgctatc ttcttttatg
     2341 gcctgttcgt caggaatagc agcgcaaata aagattttac tcattgtgtt ttaacctcat
     2401 tacagatttc agggtgaacg aatccctgcc attgctggca tttttaatcc gttggtatgg
     2461 cgttaatatg gctggcgggt tatccagccg gtgtttcgtt attcaggtac agcgatactt
     2521 tttttaccgg taggcattca ccagaaattt tttgctcgtc tcttgcctgg aggcaggatt
     2581 ctttactggc ataaattccg gtaataacat tctgtgattc acccgttata agaaaaaccg
     2641 tcatcaccag tgcaaatgct gaagtcattg acgttctccg aaaataccaa gtttaataag
     2701 ggcaattcgg gaaagaatgg aattatcatt gagcagataa ggctcatatt tccgcatatt
     2761 aatggcatcc tcagtaaacg ctttattact gagcagaaca ccaatatcaa aacaaccttc
     2821 agacgtatta acgtttggta ataacgtttc cattatcgcg tcctcaacaa tgaattttgt
     2881 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg tcgggttagt
     2941 tgatgctcgt tacgccagta aaagaccgcc ttactgcttt aactgttccg cgtgcgcata
     3001 gccgcattca ccgcatcaca aaattcactt taaaaagggc ggatatccat ttccgccgaa
     3061 tcaccagaaa agtgataaca gaggttgttg tggctggggt gtcacttaag cgtatggtca
     3121 acctgacaac ccggtgtcct caacggggga aggaataacc ccgccatact taccgccgcg
     3181 ccatttcgcg gattgccaca accagaagcg cacggtcgaa ttaaatttaa cgacgaccta
     3241 tacagagaga ctaacttcgc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat
     3301 taacctgtgg aaacctgttt ttactggcgc taatcagtta gcgtttcttg ctaaccagcg
     3361 atgcgcgcca gcttcggttt taaacgtttt acttctggta tacgtcatcg cggtaaacgt
     3421 gccgtcgtgg ttggggaata ctccgcacac cagagattca ttgttgccaa gattgagcat
     3481 atccatgttg acctcattta cccttaacgc cgggtcgcgg aactaaaaac ctgctgcgct
     3541 gttatacaaa gtgttcccgc cgtcatgttc atacgcctcg ggctggctac ttaacccctg
     3601 accactgccg ggtaactcga ggtattgccc gacgttctgt ggggcgaggt agggtggtat
     3661 gtaataaatc taaaagtatt tagtttttca gtcaaggtaa atctagatta atttaaaaaa
     3721 aaggtcggca ttgccgacct caagtaaagg acgtggcggg ttataagttg aactgtacac
     3781 cttttgctac ggcaacaatt ttacactctg gtgtaagtaa ggatgactga tagcgtggat
     3841 tgagagggct taaatacaca agcttcccat caataactaa tttctttata gtcatagatg
     3901 gttcattttt aagcggatcc ggaactatta cagcgacgat gctgccattt ttataacttt
     3961 ctcctggcct taggatcaca gtagcaccaa ctgggatact cggtgatcct gatgggttat
     4021 gcattgtgtc atcaggcatt gaaacggcaa aatcaccttc ctcgacatca aagaatgtgg
     4081 tgattctatc gacatttccc attgttttct ctccttttaa gattaggaaa gaaatcgcct
     4141 caccccacga aaggtagggg atcttggtgc ctgaggtact ttgtacaaaa gataattcag
     4201 gagacgatac tccatatagg agataggact cagtagttcc taatgcctgg gctaacttac
     4261 ttagagcttt gctacctggt tcgtttagat ctttctccca gtaccctata gtaaccccag
     4321 tcacgcctga aagcttaccc agttctactt gggtcagccc cttatctttt ctaagtctct
     4381 taagcctgaa gccaaggctt tccatcattt tctcctgcaa attgaatata aattatttta
     4441 gattgcattg acctaaaaaa aattatcttg taatctaaaa atacttagat ttgggaggta
     4501 aaatgcgagt tgatgaactt gtccagtttt ttggttctgt tcagagggtt gccgattttt
     4561 atgggataac ccgcgaagct atttacatgt ggcgtaagcg tcctggtgaa atagttccga
     4621 aggggagggc tgcggaagct gctgcatact ccaaggggaa attatctttg aacccagaac
     4681 tttacaaaaa gaaggatacc actcagggcg aaggaaaggg ggattcatga aaatcaaaca
     4741 tgagcacatc cgcatggcga tcaatgcctg ggcctatccg gacggtgaga aagttcctgc
     4801 agctgaaata gcccggactt atttcgagct ggggatgacg ttcccggaac tgtacgacga
     4861 cagccatccg gaagccctgg ctcgtaatac ccagaaaatt ttccgttggc tggataaaga
     4921 cacccctgat gctgttgaaa aaattcaggc tctgttaccg gcgatcgaaa aggcaatgcc
     4981 gcctctgctg gtggcccgta tgcgcagtca cagctctgaa tattaccgtg agatcgtcga
     5041 acggagggat cggctggtga aagatgtgga tgattttgtc gcagcggcga tcgcctgggg
     5101 caccctgact aacagtgg
//
LOCUS       DAZFKY010000253         5074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFKY010000253 DAZFKY010000000
VERSION     DAZFKY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            544..1317
                     /locus_tag="ACHKCL_004454"
     CDS             544..1317
                     /locus_tag="ACHKCL_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765284.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(1375..1929)
                     /gene="pinE"
                     /locus_tag="ACHKCL_004455"
     CDS             complement(1375..1929)
                     /gene="pinE"
                     /locus_tag="ACHKCL_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO5765285.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            1992..2453
                     /locus_tag="ACHKCL_004456"
                     /pseudo
     CDS             1992..2453
                     /locus_tag="ACHKCL_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2453..3046
                     /locus_tag="ACHKCL_004457"
     CDS             2453..3046
                     /locus_tag="ACHKCL_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5765286.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3018..3461)
                     /locus_tag="ACHKCL_004458"
     CDS             complement(3018..3461)
                     /locus_tag="ACHKCL_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFO5765287.1"
                     /translation="MDEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(3482..4192)
                     /locus_tag="ACHKCL_004459"
     CDS             complement(3482..4192)
                     /locus_tag="ACHKCL_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765288.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALQNLGLGEAAKRDVGTGENQIPDMASFASGNGWMKLPNGKILQYGRGAVTP
                     TLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAYRT
                     SSTSDTVSMNYSWWAVGE"
     gene            complement(4192..4440)
                     /locus_tag="ACHKCL_004460"
                     /pseudo
     CDS             complement(4192..4440)
                     /locus_tag="ACHKCL_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            complement(4441..4503)
                     /locus_tag="ACHKCL_004461"
                     /pseudo
     CDS             complement(4441..4503)
                     /locus_tag="ACHKCL_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            complement(4500..5074)
                     /locus_tag="ACHKCL_004462"
                     /pseudo
     CDS             complement(4500..5074)
                     /locus_tag="ACHKCL_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
BASE COUNT         1318 a         1234 c         1150 g         1372 t
ORIGIN      
        1 tgccagccca tgtttgcctc cgggtattga ccccttctct acgcaacttc agttcccacc
       61 accaactttg cggcagcttt gtaggatcaa tgtctaaaag aataatggtg accgataaga
      121 aaacgactga ataactgcag attttcgctc aaaaccttcc tgtcagatcc atagcgaatc
      181 aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct tcaagaatat
      241 tctacgattg ttctgtttag gaaaagcaag acgggaagtc gggagataag tcattgataa
      301 agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc ggttttcgag
      361 accgacccga tcatcaaacg aaacataaaa ttagctcaca ttatgaggaa aggtatcttt
      421 ttgcgctatg taaattcaaa gggttagcct cattttcccg atggctttct caacactact
      481 agttgtgagc ccttgcaatg ttcattaata tacgtctcac aaataattca cagatattgc
      541 aaaatggata ttactgagtt tccttctgga gtaattgaac accttggctg gtatgtatac
      601 cgattgattg atcctaggga cggaagcacc ttctatgtag ggaaaggcaa aggtaaccgc
      661 gtatttgccc atatgcgcgg tgaagtggca gcggctgatg atgacgactt actgagcaac
      721 aagctaaagc aaatcagaga aataagatta gcgggacttg aagttatcca tgtcatccat
      781 cgacatggaa tgactgatga aaagacggcg tacgaagttg aagcagcact tattgatgcc
      841 taccctgggt taacgaatat catgaatggt gctggcagca atgaattcgg cgccgcgcat
      901 gtcaaagagt tgatagcaac atatcaaccc gaaaccataa catttcatca taaagcatta
      961 atgatttccg ttaacagaag tgcaaaagat tcagagcttt atgatgcggt tcgatttagc
     1021 tggcgcatta atgtctcgcg cgccagtaaa gcagaagtca ttcttgctac tgtaaggggg
     1081 atcgttcgag gagttttcat tgctgataaa tggctaaaat caacgcgtga acattttcct
     1141 acgatgaaat actgggacga agaccctgac tttgaggcaa cacaaagctc tcgctatggt
     1201 tttgaaggtc gagaagcccc acctgaaata gcaaaccttt atcttgggaa aaaaatacca
     1261 gatgaattaa gaaaaaaagg agctatgtct ccagttcgtt actcacctaa tttttgaatc
     1321 ttcaagtggt aagcataaac cgcagcacga tcttgcatac gacgtgctac ggtttcattt
     1381 atctccggcc ggaaacttct tatacagtgt cgatacacca acatcataga tgatcgctac
     1441 cttctggcga ggaattcctg ctgcaattaa tcgtccggcc tgcgcccatt gttctggtgt
     1501 aagtttggga cgacgtccac caattcgtcc ctgtgcgcga gcagcttcca gtccagcttt
     1561 tgttcgttca acaatcagtt ctcgttccat ttcagccagg gcccccatca catgaaagaa
     1621 aaagcgcccc attggggtac tggtatcaat agcatccgtc aggctgcgaa aattaacgcc
     1681 acgttcgcgc aactcctcaa ccagaatgac cagatgccgc atactgcgcc ccagccgatc
     1741 cagtttccag acaaccagag tgtcacctgc cgataatgtc ctgagcagtt ttttcagtcc
     1801 cggcctttcg gactttgtac cgcttatctt gtcttcaaaa atcagctcgc atcctgcaca
     1861 gttcagcgca ttacgttgta gatctgtgtt ctggtcattt gttgacacac gtacatagcc
     1921 aataagcatg gtagatctcc ctgacaaaag caggaatgat gccatttgct cgttatttct
     1981 gcattttcat aaacgttggt ttgggagaag gtgctccagc tattggcgtt ccgttcttct
     2041 ggccgtccgc tgcaatgcca aatactgtaa tcgacagctg gtcctgtatg gtgtttttga
     2101 agttcaacgg cgcgaaattc tctgccactg attatcctgt gctggcgaaa gtgtttcctt
     2161 cgctggtatt acctgaagcc cgcggtgatt tcattcgtat ctgggatgac gggcgaggtg
     2221 cagacggtgg tcgcgaatta ttaagctggc aggcagctac aaacttttct cagtttgccg
     2281 ggaatatagg cgaaggtgcg ggacacgcaa ttaactttca tgatggcatc gccggaaatc
     2341 agccaggatt ttcacgattt aatttcacca gtaactctgt gggtgatggt gtgaattttg
     2401 ttgcagtcag accgcgaaat attgcattta actttctggt gagggctaaa taatgaaacc
     2461 tgtttttgat gaaaatgggc tggctacagt gccgggcgat atgcgttgtt tttattatga
     2521 tgctgaaaca tctgagtata cgggctggtc tgatgaatat attaatactg gcgtaagtat
     2581 gcccgcctgt tccactggta ttgaccctgg cgaaaacatt ccgggaagag tggcagtatt
     2641 tacaggtaag ggatggagcc atgaagaaga ccatcgcaat gagactgttt actcaatcga
     2701 aaatggcgca gctgttacag tggattatat cggtgccatc aaagacggtt atgtcacgat
     2761 ttcaccgtta acgccatacg ataaatggga tggtgagaaa tgggtgacag acactgaggc
     2821 acaacacagt gccgcagtag acgcggcaga agcacagcgc caatcactga ttgatgcagc
     2881 aatggcttcc attagtctga ttcagctgaa gttacaggcc ggacggaaac tgactcaggc
     2941 agaaacaacc cgacttaacg ccgtgctgga ttacattgac gcggtgacgg caacagatac
     3001 cagcaccgcg ccggatgtca tctggcctga actgccggag gcgtaggcca ttcaatatct
     3061 ggcgcaccgg aagtatcgac cagctccagt gcgtccagat aatccagcca caaattatat
     3121 tgcgtcagtt cgtcaccttt cagacgacca atagccgctt taccgggcca ttgcttactg
     3181 ttcatgtatt cgttggcctg gttaattagt agctgtcttt ctgattcagt aatttcaata
     3241 agctcttcat gcgtgggtgg aggaatatct gcccacgcag gcagcccatc atctccggca
     3301 atacggattt ttccttgtgg cggttcagcc ataaactcac tgataatatt ttgatttact
     3361 tccttagcgt ctgataaatc ccatccctct gatttatatt tatcaatcat atccacaggg
     3421 aaaaaagcat tatgccttgc gctataaaca tattcgtcca tataaatcac cctgaataaa
     3481 attactcacc aacagcccac caactgtaat tcatcgatac cgtgtcgctg gttgatgacg
     3541 ttctgtaagc agaattaaag ccggttaacg ttgggccttc tgcagtcatc acgaaccctc
     3601 gcccagcgcc taaaggcgca ccgccatcac cagaatgagt aagcatggcg cagtccgctt
     3661 ttttggggaa agggatgctg aatgtaattc tcattgtttg cgtcgataat gtcggcgtaa
     3721 ccgcaccacg accatattgc aggattttcc cgttgggtaa tttcatccat ccattaccac
     3781 tggcaaaaga ggccatgtcc ggtatctgat tttcccctgt ccccacatcc cgttttgccg
     3841 cttctcccaa accaaggttt tgaagagccg ttttcaccgt gccgtccgat ttgatatcgc
     3901 caaacggatt cttgcggctc aggtattcaa cagcaaaccc cgatcccagc aattcaacaa
     3961 aaccgggcag atcaccatta tcaagcacat cccgttgcgt tttatcactt acaaactggg
     4021 ccagagctgc agcaataaag ctggcctgcc gaataacctt attgacttgc gcactggatg
     4081 ctttccctgc tgtaaatccg gatataagcg caggcaacgc ttcccattcc tcctgcgaca
     4141 taacattggc atttcgatca gttgcaaacg ctttaaagtc atttttcgcc atcagagtaa
     4201 tactccccat gcccctacat caaaaccact gatgaattcg ttatccatat caaaaccaaa
     4261 aaatttagag ccttccgatg gagtttccac cgaaggtgtt tcaatgcccc ccgcccatac
     4321 cccggcggct tttactgtta gatacccctg tttaattgcc gcaattaact cacgcgatac
     4381 atctgaaata tcagtatcag gaaagaccca gaccgatatc gtcatgtcct ggttatcgat
     4441 gttggcgatc cgacgcatct gctcacggtc aaagttaacc atctgtgcgg cgatgttttt
     4501 cataaatcca ccccgtaaat ccagtcagtg tttgtcaggt cgagttttgg tttgctggct
     4561 gtcacgcctg cctgttgctt gttacggttg atttcgagct gggtccactt gtcgcggagt
     4621 ttggccggac tcagcacgtt accggaccag aagttgtcct ggcaggccca gcggaaaagc
     4681 acacacatat cgcggtggtt acgtccgtca cgttcacgca tcaggcggat atcgttagcc
     4741 cacccagcaa aattcggttt tctggctgat ggcgcgatgg tcttcaccat gtcaaacatc
     4801 cactctgcgg cggtcaggtc ttctgctgtt ccccacttgc tgccgctctg aattgcagca
     4861 tccggtttaa ccacagaaag atcgttttct ggctggtcag aggattcgcc agaattctcg
     4921 gacgaataat cttttctttt ttcttttgta atagtgtctt ttgtgtcccc ctgttttgag
     4981 ggatagcaat cccccaattt gagggatagc aatcccccaa tttgagggat gttttatccc
     5041 tcgttttagg ggattttccc tcgttttgag ggat
//
LOCUS       DAZFKY010000254         5052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFKY010000254 DAZFKY010000000
VERSION     DAZFKY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..384
                     /locus_tag="ACHKCL_004463"
     CDS             <1..384
                     /locus_tag="ACHKCL_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765289.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHKCL_004464"
     CDS             419..1117
                     /locus_tag="ACHKCL_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5765290.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHKCL_004465"
     CDS             1139..1363
                     /locus_tag="ACHKCL_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765291.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHKCL_004466"
     CDS             1360..1716
                     /locus_tag="ACHKCL_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO5765292.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHKCL_004467"
     CDS             1749..1901
                     /locus_tag="ACHKCL_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765293.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHKCL_004468"
     CDS             1898..2209
                     /locus_tag="ACHKCL_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765294.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2908
                     /locus_tag="ACHKCL_004469"
     CDS             2336..2908
                     /locus_tag="ACHKCL_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765295.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASLASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQ
                     AGNFRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGS
                     CLDEAEQELIVTINEYASQIAVEATQGENQ"
     gene            3018..3122
                     /locus_tag="ACHKCL_004470"
     CDS             3018..3122
                     /locus_tag="ACHKCL_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765296.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3366..3521
                     /gene="hokD"
                     /locus_tag="ACHKCL_004471"
     CDS             3366..3521
                     /gene="hokD"
                     /locus_tag="ACHKCL_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5765297.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3689..3967
                     /locus_tag="ACHKCL_004472"
     CDS             3689..3967
                     /locus_tag="ACHKCL_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765298.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3969..5018
                     /locus_tag="ACHKCL_004473"
     CDS             3969..5018
                     /locus_tag="ACHKCL_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5765299.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
BASE COUNT         1357 a         1135 c         1409 g         1151 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcactgg catcagtatc ggatgaacgg gcagcctatg aattatttat
     2521 ggagaagcgt ttcggggaat ctgtagatcg ccgcagagca aaaaatggcg atagagaata
     2581 catggcatgg gatatggcgc ttggctggat tatctggtgt caccgcgccg ccatgcttca
     2641 ggctggaaac tttcgggaaa ataagggttc gtcaaccaat aattttcgga taatctcgga
     2701 aacgtcaacc aactctccgg caatccctga tgaggtgttg tccgcaatcc tgaaggtcgc
     2761 caggcttcgt gcagatttcg atgcttctga agtggacagg cgaggtatcg gtagttgtct
     2821 ggatgaggcc gagcaagaac ttatcgttac cattaacgaa tacgccagtc agatcgcagt
     2881 agaagcgaca cagggggaga accaatgagc cggccagatg catttgcagg cgttgctata
     2941 gctattgctt ttctagtata tgttatttgt cggtgggggt aaaaacgttc gccgggattc
     3001 acaccaaggg agggaatatg tcggatgata tttcactggt aatggaaggc gctctggctg
     3061 ttattgctgt tgtgggtgtt tactgcctgg ttgtgttttt gatggagcgc ctggggaact
     3121 gaattcattc cgtatgggaa ttcccatatc gggcaaaacg gtttgctgta aagcgagagt
     3181 taagtagaat tgctgcgggt gcttgaggct gtctgcctcg ggcatgccac cgtaaggcag
     3241 acagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat taatctgagg
     3301 cccaatctat gcttcacaaa cgtaggttag cctcttacgc gccgaaaggc aaggagaagc
     3361 aggttatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca
     3421 tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga
     3481 cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagaccagg cgggggagaa
     3541 atccctcgcc accgctgatg tgtcaggcat cctcaacgca cccgcactta acccgcttcg
     3601 gcgggttttg ttttttctgg tcgttctggt ttacaatcca tccgtcagcc tgaacaactg
     3661 gcacctgctg cgccagcaga gaaaacagat ggcgcacgat accaaatttt acaattcgga
     3721 taactctgcc gcccctgcca gcaggcacgg gcggcgttct catgcattca aatctgactg
     3781 gtatcagcac gacccctgca ccgaagaaca ggctgaatgg ctgattcagt gttaccgcag
     3841 gcgcggatac gaggttaaga aagccctcag tctcgattat cgtcactgga taatctccgt
     3901 caggcttcct tactccgaac gcccaccgcg tccgtcccgc acattccagc aacgcatctg
     3961 gaggtaacgt gcgggtatta cttcgacctg ttctggtacc ggaactcggg ctggtgatcg
     4021 ttaagccggg ccgtgaatcc atgccggtat tccacaatac ccgggtactg gtggagccgg
     4081 aaccgaaaag catgcgtaat ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg
     4141 cggaggataa atcattactg ccatttttca gcgacgaacg agtgattcgt gctgctggtg
     4201 gcgctggcgc actgtctgac tggttactgc gccatgttaa atcctgccag tggccacacg
     4261 gcgattatca ccacagtgaa accgtcattc accgttatgg taccggcgca atggtgttgt
     4321 gctggcactg cgacaaccag ttgcgtgacc agacctccga atcacttgag caacttgctc
     4381 accaaaacct gtcagcatgg atgattgacg tcattcgtca cgcaatgaat ggcacacagg
     4441 agcgtgaatt atcgctggct gaattatcct ggtgggcggc ctgcaatcag gtggtggatg
     4501 cactacctga ggcagtagcg cgtcgttcgc tgggattacc agcggaaaaa atccgctccg
     4561 tataccgtga gagtgacatc gtaccgggag aacagacagc catcagcata ctgaagcagc
     4621 gcacaaaaaa tattgcgctg ccacttcacg tccaccagca acaaaatcca ccacagaaaa
     4681 aaacggttgt cagtatcgcc gttgatccgg agtctcctga atcgttcatg aggcggccta
     4741 aacgttgccg ctgggttaat gagaaataca cgcgctgggt aaagacacag ccgtgtgcgt
     4801 gttgtggtaa gcctgctgac gatccgcatc acctgattgg tcatggtcag gggggaatgg
     4861 ggacaaaggc ccacgatatt ttcacgctac cgttgtgccg ggagcaccac aacgaacttc
     4921 atgcagaccc gctggagttt gagaaaaagt acggctctca gattgagtta atttttcgtt
     4981 ttcttgatca cgcctttgcg actggcgtgc tcgggtaaaa gaggtgactg atgctcatag
     5041 atttggtttt ac
//
LOCUS       DAZFKY010000255         5002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFKY010000255 DAZFKY010000000
VERSION     DAZFKY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..172)
                     /locus_tag="ACHKCL_004474"
     CDS             complement(<1..172)
                     /locus_tag="ACHKCL_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5765300.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLY"
     gene            complement(218..679)
                     /locus_tag="ACHKCL_004475"
     CDS             complement(218..679)
                     /locus_tag="ACHKCL_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5765301.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(924..1136)
                     /locus_tag="ACHKCL_004476"
     CDS             complement(924..1136)
                     /locus_tag="ACHKCL_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5765302.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(1269..1829)
                     /locus_tag="ACHKCL_004477"
     CDS             complement(1269..1829)
                     /locus_tag="ACHKCL_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5765303.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1932..2792)
                     /locus_tag="ACHKCL_004478"
     CDS             complement(1932..2792)
                     /locus_tag="ACHKCL_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5765304.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            complement(2851..3597)
                     /gene="traX"
                     /locus_tag="ACHKCL_004479"
     CDS             complement(2851..3597)
                     /gene="traX"
                     /locus_tag="ACHKCL_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO5765305.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(3617..>5002)
                     /locus_tag="ACHKCL_004480"
     CDS             complement(3617..>5002)
                     /locus_tag="ACHKCL_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO5765306.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
BASE COUNT         1099 a         1528 c         1119 g         1256 t
ORIGIN      
        1 catataactt tccggtgatt aattctgcta accggtggtc tgctgctgaa ttaaatgatt
       61 cacgttcact ggtcgataaa ctgtctctgt ttttatgaat aattttttcc agcgtattga
      121 ctgatgaaca tcgccgtaat tgatacagcc attcctgttt tgttttttcc atttttattc
      181 ccgctgagaa cagatgaatg ataagattgc catcgcttta tttacggtgt ctccatatcc
      241 aggggggaac cggatcagca tctgaccaga gcccacgttt ttgttgtctg gcttcgttct
      301 gaaggacagg cagcacggga tctgtgttgt actgctcata tacccaggcg gccccggccc
      361 ggaccataaa ctgattaaca ttcattccgt ctggtgcata aacctgcccg agcatacgac
      421 cataacgatc tcgctgaaaa tatgtgacgg taaccgtttt tcctgcaacc agtgatttca
      481 tcatgtctgt cgaccaccgg ccataatcct gtttcttttc cggcgcatca atatttacca
      541 gcctgatccg caccgccttc cgtgagtcca tgacctcgat ggtatcaccg tccagtacac
      601 gaacaacccg tccatgaata tctgcacaca ataccggcca tgaaaagata aataatacca
      661 gtggaatata tttcctcatg ttattctccg tatcagataa atagcacctg tatttcaggc
      721 atcctgaata ttgcagcaca cacttaataa caccttattt tgtgaaatta atcacaaaat
      781 ataaaatcac gacttaatac ggaaaaaact ctgtgctact cagaactggc cggtcatact
      841 gcccgaccgg atattaaaaa aagcaaatct catgcattat tcagccttct gaatccctgg
      901 agtaatgaag gctgcttttt tcgttacccc cttcctcggc agcgccggtg gcagtgttca
      961 gccctttttc gggcaaactg ctgccagtca gtccggggtg caacaccggc agcccgtcgc
     1021 catgcttctg ccgcttccat ccagcgtcca ctgacttcca gaataacggc ccggcttccg
     1081 gcacgctggt aacgccagac ctgaccgttc cgtgaactgt ttctgaatcc attcatcttt
     1141 atcactcctc tttaacccgt cagtattatg gtgttaaata atctactgaa ttggcttcct
     1201 ttcgggtacg gaagttattt ccttttaagc cttatttccc tgcctcctct ctctgaggga
     1261 ggcttttttt atttctcatc aagcacggcc tgaagttctg cctttatccg gttctggcgg
     1321 cggattttat ccagacgcgc tgccgcatac gcttcctcct cctgagaaat atgctccgtc
     1381 acataccctt ccgtgtcata ccggcaggca ccggctttca tggcacacag atagctttct
     1441 gaacgggtga tggccttcag cgccctgcgc agctttttat gcgagagcgg gatattccgc
     1501 tgcgccacgt cttccagtaa cacgtcccgg ataccgcagg ccagaagccg gggcgtgtca
     1561 ccgtcaaata atcccggcca ccagggtttc agggtgttca cggcctcatc ctgcgtgggc
     1621 aggttcagat aaatggacag cgcctgtctg gcctgcgctt tttttgccgc cagctccgct
     1681 tcccgggctg ctttctcggc cagcttctgc tttttcacct tccattttgg tggcgtggtg
     1741 acattgatga tggttttccg gctgcggaca ggcgtctctc cttccgtttt ccgcttcagt
     1801 gtcagtaccg gtcgtttttg ctctgccatg cttactctga ccttcgtgaa aatatgccct
     1861 gcaggccgtt gaaacggctc atcgtaacag gaagttaatt tcaacgacct ttaacgtata
     1921 aatactgaca attaaagata ttgtttaaac cattgtgtct gcacagctac tacacgttca
     1981 aaaaacgcgc cctcataaat atcataatgg catgcatcag cttcctcgta taattcctta
     2041 gtaccagaag caacagcatc atataatgct cttccctgtt ccggtggatt aacgctatcc
     2101 tggccggcaa taacaaccaa aactgggcac tggacgctgg ctgcactctc tgccggttta
     2161 tattgcaacg tttccatgac cgtaaggaaa ggtattttta tatccatttc gggatgccgg
     2221 gctttgattt tctcgaagaa taccttcgat tcatcatcac ttagtacccg tgtaaccccc
     2281 acaaacatct ctttcccggt gtttttcttc ttctctgcca ttttattcag cgtggataga
     2341 aaagacgctc tctccgattc attcatctct ccggtaacca gaacatcacc gtcagcaaaa
     2401 gccagctggc tgacaataca ttttacgcgt tggtcctgcg cagctgcact gaagacatgc
     2461 ccccctccca gcgatgttcc ccataaccca atacgctgat tatcaataca ttcctgcttt
     2521 tctgcccaat tgataactga aataatatct tcggtctgca ttgcaggaac cagacgccct
     2581 cgttctccat cactctcacc gaatcctcga taatcaaaag taatcgttgc gaatcctgct
     2641 tctgtgaagg cattcgcaaa gcaagggagc agaacattcc tgataccgca aaacccatgg
     2701 cataagataa tcaaaggatg ttttatattt ccttcaggaa cacgaaatgt cagggcaata
     2761 ccttcagaga gtttgtgatc cgtaattttc ataaatatcc tgtgttatgt tgtttattaa
     2821 taagcagaat cagcgtcagt atcataccag tcacaacgcc agaatgccca gcacagccag
     2881 atgacagcca tagaacgtcg ggaaaaaatc tccgggccag aaacggggta atgatttccc
     2941 tgcacaggac accagaccaa cggtcagcac cgtcatcaca agaccagcta cagccgccgc
     3001 atcactggag gcaaggttaa gtgccggaat cacggccagc aggcatgcaa ccagcaccag
     3061 acgttccgtt ctgtcttccg cccggtacag ccggtggctg actgccagca tcagcagccc
     3121 ggcaatgccg taactggtgc cggacaaagg cccccacagt gccatcagga gaatggccgc
     3181 tgctgtacgc cacccactgc gcgtttcaca ccacgtcagc acctgtgccg ccactgcaaa
     3241 ggcaaacagg atattcccct cataccacgg aaagcctgcc agataatacg cgaactgggc
     3301 aataattccc catccccaca gccggttaat ggccgactgc ctgatatgcg catggcggga
     3361 cagattcagc ccccacacca gagcaaacaa cggaaaggct ccacgcccgg ccagaaacat
     3421 ccacgtctgg tccagatgca gaatgcggtt tgcgtggtcc agtaccatca gcaccagtgc
     3481 caccgttttg atgatatccc gctgtccggg tgaccagacc agcaacgact gcaaccaggt
     3541 atcggtccgg gcagccagtg aatcgttacg tgtcgtgttc gtgttatccg ttgtcatcag
     3601 tgtttgtctt cctgtatcag tctccgccca gggttttctc tttctgcagg tcacgaacca
     3661 tatcccgctc catctgctgc agtcgctcct gcagcaggtt ttcccggaca acctcacgga
     3721 ccgcctcccg ctcccgttgt ggttcacgca gtacactctc cggcagcgcg gcttcccgtt
     3781 cagaagtgac agcccgttca cgctcctgcc cggcaatctc cctgacagcc tgctctgttc
     3841 tgccgtccgg caggtcaggt ttgttctctg ccattttacg gacaatttca tctgcgcggc
     3901 gttccgtttc acggcggatg gcctcttcag cctgccgctg tgccagtacc tctgccgtga
     3961 ccggctcgcc atttccggct cccggctgga cgctgttgtc cgggatatcc ccccacacgc
     4021 gaccaccggt gatggcaccg ggattccacg gacgaccttc accggcgatt ctcaccacca
     4081 caccgctgtc aggattatca cgggcaatcc ggacaccatc ctgcatatta tcagccagca
     4141 ggctctctcc gttacggctg ccctgcaggg ccacgaactg cgcatccccg ctgcctttca
     4201 cccgtccttc accactgaat ccccgcagcc cgtttccgtc atccgtggtc agcgggttca
     4261 gccagatacc ggcagacttg ccgttacggt caaacgccgg cagtgccaca tacggctgtg
     4321 gatatttacg tcccggagca ataaaccgtg caggactgtc tcccccggca agccccgcct
     4381 gacgaagaac ggcacgccct gccgccacgt cccgcagctc ccgcgccgta ctgaacagcc
     4441 gctctgcatt catgacctcc cggtccggtt tcggttcaaa cacatcgtgg gctgttcctt
     4501 tctgtacggc attgttaatg gcatccgtcc agccctgacg gttatcggtg tacacctgca
     4561 catgctgctt catacgcgac agggccacgt aggctgactc aaagccggcc atcagtttcc
     4621 ggctgccttc cgtaccttca agcgcgatgg caaaggtttc acttgccccc tgcgcaccgt
     4681 gagcggtgat ggcataggcc aggtcaatat gttgctctgc ccgctcctgg gcggggcgaa
     4741 tcacccgggt ctgctgtccg cccgacagcg tgacactgtc accggaaacc gctgtcaccg
     4801 tccagacgct gttggccaca taaccacgcc cccggtcact cttcgtgaag cgcatccggt
     4861 caccggtccc caccctgatg gtgtccgggg ggtacagtgt gacgccttca gccaccgcct
     4921 cccggggcga aatcagccgc gtgttacctt ccgcatcctg cagagttatc agcccgtcat
     4981 ccctgctgat accggcaatc cg
//
LOCUS       DAZFKY010000256         4995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFKY010000256 DAZFKY010000000
VERSION     DAZFKY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..1681
                     /gene="dpiB"
                     /locus_tag="ACHKCL_004481"
     CDS             23..1681
                     /gene="dpiB"
                     /locus_tag="ACHKCL_004481"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5765307.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1650..2330
                     /gene="dpiA"
                     /locus_tag="ACHKCL_004482"
                     /gene_synonym="citB"
     CDS             1650..2330
                     /gene="dpiA"
                     /locus_tag="ACHKCL_004482"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5765308.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2371..3756)
                     /gene="dcuC"
                     /locus_tag="ACHKCL_004483"
     CDS             complement(2371..3756)
                     /gene="dcuC"
                     /locus_tag="ACHKCL_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5765309.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            4345..4905
                     /gene="pagP"
                     /locus_tag="ACHKCL_004484"
     CDS             4345..4905
                     /gene="pagP"
                     /locus_tag="ACHKCL_004484"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5765310.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1379 a         1117 c         1253 g         1246 t
ORIGIN      
        1 gggacaaccc gcaaggaaaa caatgttgca gcttaacgag aataaacagt ttgcattttt
       61 ccaaagactg gcatttccgc tgcgtatctt tttgctgatt ctggtgttct caatatttgt
      121 cattgcagcc ctggcgcaat attttacggc cagttttgag gactatttaa cgcttcatgt
      181 acgcgacatg gcaatgaatc aggcgaaaat tattgcctcc aatgacagtg tcatctctga
      241 ggtgaaaacg cgtgactaca aacggctggc gaccatcgct aacaaattac aaagagatac
      301 cgattttgat tatgtggtga ttggggaccg gcactcgatc cgcctttacc atcctaatcc
      361 ggagaaaatt ggttatccta tgcagttcac caaacagggc gcgctggaga aaggggagag
      421 ctacttcatt accgggaaag ggtcaatggg gatggcgatg cgcgccaaaa cgccaatctt
      481 tgatgacgat ggaaaagtca tcggcgtggt gtcgattggc tacctggtga gtaaaatcga
      541 tagctggcgg gctgagtttt tattaccgat ggcaggcgtg tttgtcgtgc tgttagggat
      601 tctgatgttg ctgtcgtggt tcctggcagc gcatatccgt cggcaaatga tgggcatgga
      661 gccaaagcaa atcgcacgcg tggtccgtca gcaagaggcg ctgtttagtt cggtttatga
      721 agggctgatt gcggtggatc cgcatggtta cattaccgcc atcaatcgta acgcaagaaa
      781 gatgctgggg ctgagctctc ccggacggca atggttgggt aaacccattg ctgaagtggt
      841 caggcccgcc gatttcttta ccgaacagat tgatgaaaaa cgtcaggatg tggtggctaa
      901 ctttaacggt ctgagcgtta ttgccaatcg ggaagctatt cgttcaggtg atgatttgct
      961 gggggccatt atcagctttc gcagtaaaga cgaaatttcc accctcaatg cgcaactgac
     1021 gcaaataaaa caatacgttg agagccttcg tacattgcga cacgagcatc tcaattggat
     1081 gtcgacgctc aatggtctgt tgcagatgaa agagtatgat cgcgtgctgg cgatggtgca
     1141 gggggagtct caggcccagc aacagcttat tgacagcctg cgcgaggcgt ttgccgatcg
     1201 ccaggtggcg gggctgcttt ttggtaaagt gcagcgcgcc cgggaactgg ggctaaaaat
     1261 gatcattgtc cccggtagcc agctttcgca actgccgcca ggactggata gcaccgagtt
     1321 tgcagccatt gtgggcaatt tacttgataa cgccttcgaa gccagcctgc gtagcgatga
     1381 aggaaacaag atcgttgaat tattcctcag cgatgaaggc gatgatgtgg tgattgaagt
     1441 cgccgatcag ggctgcggcg ttccagagtc tctacgagac aaaatatttg agcagggggt
     1501 cagtacgcgt gctgacgagc ccggtgaaca tggcattggg ttgtacttga ttgccagcta
     1561 cgtaacgcgc tgcggtggtg ttatcactct cgaagataat gatccctgcg gtaccttatt
     1621 ttcaatctat attccgaaag tgaaaccaaa tgacagctcc attaacccta ttgatcgttg
     1681 aggacgaaac gccgctggca gagatgcatg ctgaatatat tcgtcacatt cccggattca
     1741 gtcagatatt actggcggga aacctggcgc aggcccgaat gatgatcgag cgttttaagc
     1801 cggggctaat cttgctcgat aactatcttc ctgacggtag agggattaat ttactgcatg
     1861 aactggtgca ggcgcattat cccggcgacg tggtgtttac cactgcagcc agcgatatgg
     1921 aaacggtgtc tgaagccgta cgttgtggtg tatttgatta tctcattaag cccattgcct
     1981 atgaacggct ggggcaaacg ctaacccgtt tccgccagcg taaacatatg ctggaaagta
     2041 ttgatagcgc cagccagaag caaattgatg agatgtttaa tgcctatgct cgcggtgaac
     2101 ccaaagacga actgccgacc ggcattgacc ccttgacgct aaacgcggtg cgaaaactgt
     2161 ttaaagagcc tggtgtgcaa catacggcag aaacggtggc gcaggcactg accatcagtc
     2221 gcaccactgc caggcgttat cttgaatatt gcgccagccg ccatctgatt attgctgaaa
     2281 ttgttcacgg caaagttggc agaccgcaac gcatatacca cagtgggtga catgataaag
     2341 ccggaggaaa cttgcctccg gcattactat ttacttgcct gtgaccgctg ctgccgttcc
     2401 tggcaccatc agctctgtag caacgataac aatcaccaaa ccaacaagca ccggtaccga
     2461 ggtgcgtttt acgacttcaa acggcgagat cttcgccatc ccggcaaccg caacgactac
     2521 gccagaaacg ggcgaaaggg tacggcccag gtttgacgcc tgcagcatcg ggatagtcaa
     2581 atacgccggg ttaatgccgg aagagtgcgc cagtttcggg atcatctcaa caaacgcata
     2641 aaacggcgca ttgcctgaac cggtcgtgac tgccgccagc attgtcagaa tcaccaatac
     2701 cagcatcagg atgatactcg ccgaaccaaa cgaggtagcg atagaaatca gactttgaat
     2761 aaagccgatg gtgctaagcc cctgagcgaa taccccagcg gcaaccagca gcatcaccac
     2821 gttagcaaac gcatctgcca tcccgcgata agccacttcc agaccagaga aaactttctg
     2881 ggtattaaag ctgcggagga actccagaat ggaggcaatc agcatacaaa tcaccagaat
     2941 agtgatgatg tgtaattgcg gaccccattt accgtcaaaa atcagtacac cgatgatcgg
     3001 cgtgaacggc aaaatggcat aaaacgcagg ggcagtggtg gtgatttcac tgacatctaa
     3061 catttcatga gagatgtgct cttttttatc cagataacgt tgccagaaga agtgggcgat
     3121 cgccatgccg ataattgcag caattgagat aggcagcgtc gttttgaagg cgaagtcaat
     3181 cagcgacatt tcggaagctt gcgccgccag caccacatcc cctgaagtcg gtgcgagaat
     3241 aatcgccgcc ggggaggcac aaatggcagc agctgcgcca cgactgatac caacgtttac
     3301 catcaccgga aatagggttg ccatcagcaa aacacccaga ccggttgcgg aagagacggc
     3361 cagagacatt agacaggcga caaaataggc ggcaatcatc agcaggtaag gggagttaat
     3421 atactgcaat ggttttgacg ccagcttgac caccatatca ttcgcgccga tatgggtcat
     3481 gtaagcggca aatccacaca gcatcataat catcatgccg aggtcgccgc cgcggctcat
     3541 tagtaatatt ttaacgtatt caacgatatc cgtggcgctg tagcctgttg aagcctggct
     3601 ggacggtaac actttgtgcc ccataatggc actgataatc agcaataaca ggccaccgac
     3661 aaataacaca ccagtggcgg aatacccttt aatgatgtag cgagctacac ccacaataac
     3721 cacaacccca ataaggagtt caatgaatgt cagcataatt tttcctgtct ccaggcccca
     3781 aagtaaataa taaaaaattc ctaaagctta aggaaaaaat atgcccaata aattggcgat
     3841 gaatgctgat taaaatcaag aaaaactgcc attaagacat tgaagttgct gtttttatac
     3901 gtagataaca aaacgcatat ttagcggttg gcaattatca cataataatg tgtgtaaatt
     3961 gatggaggat tgatgcaaat ttgttaattg caatggtgtt agctgcggtt gtgtgctcaa
     4021 aaaataatct gcaatgggcg tgggttgacg atatataata tattccctgt taagatgatt
     4081 tttggtatct tattaaaaaa taagtcattt tttcttggtt gcattttgtt gtcagaaaca
     4141 actagctttt tcttttatga ctattcccat cgcagaaaac gatgtgtcat tttaaatcga
     4201 tgcgcggaaa tatttaactt gaacaagcgg aaataaatag agcagctatt cagattattc
     4261 tttatgttgg ggctgttaag gttatgttaa ttgtagcttt gctatgctag tagtagattt
     4321 ttgataaatg ttttatggtc acaaatgaac gtgagtaaat atgtcgctat cttttccttt
     4381 gtttttattc agttaatcag cgttggtaaa gtttttgcta acgcagatga gtggatgaca
     4441 acgtttagag aaaatattgt acaaacctgg caacagcctg aacattatga tttatatatt
     4501 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
     4561 gagcgaccgt ggggtggcgg ttttggcctg tcgcgttggg atgaaaaagg aaactggcat
     4621 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
     4681 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
     4741 accgctggcg taacggcacg cgataactgg aattacatcc ctctcccggt tctactgcca
     4801 ttggcctccg tgggttatgg cccagttact tttcagatga cctacattcc gggtacctac
     4861 aacaatggca atgtgtactt tgcctggatg cgctttcagt tttgagacaa atgaagtttt
     4921 agtaacttct ttaaaatcaa taactaaaat aagcaacatc aaaaataacg cgatttttat
     4981 cactttttag taaag
//
LOCUS       DAZFKY010000257         4986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFKY010000257 DAZFKY010000000
VERSION     DAZFKY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..310
                     /locus_tag="ACHKCL_004485"
     CDS             41..310
                     /locus_tag="ACHKCL_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5765311.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            596..667
                     /locus_tag="ACHKCL_004486"
                     /pseudo
     CDS             596..667
                     /locus_tag="ACHKCL_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(678..926)
                     /locus_tag="ACHKCL_004487"
     CDS             complement(678..926)
                     /locus_tag="ACHKCL_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5765312.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(1154..1219)
                     /locus_tag="ACHKCL_004488"
                     /pseudo
     CDS             complement(1154..1219)
                     /locus_tag="ACHKCL_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1441..3126
                     /gene="btsS"
                     /locus_tag="ACHKCL_004489"
     CDS             1441..3126
                     /gene="btsS"
                     /locus_tag="ACHKCL_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5765313.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3123..3842
                     /gene="btsR"
                     /locus_tag="ACHKCL_004490"
                     /gene_synonym="yehT"
     CDS             3123..3842
                     /gene="btsR"
                     /locus_tag="ACHKCL_004490"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5765314.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3889..4359
                     /gene="yehS"
                     /locus_tag="ACHKCL_004491"
     CDS             3889..4359
                     /gene="yehS"
                     /locus_tag="ACHKCL_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5765315.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4401..4862)
                     /gene="yehR"
                     /locus_tag="ACHKCL_004492"
     CDS             complement(4401..4862)
                     /gene="yehR"
                     /locus_tag="ACHKCL_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5765316.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT         1254 a         1109 c         1292 g         1331 t
ORIGIN      
        1 gatgaagtgg gcagtgatac aggcagaaca ggagaatgac atgaacatac taaaaaaact
       61 tatgcagcgt ctgtgcgggc acggaaagca tgatgaccgt gaagacgggg agttacttac
      121 agcacaactg cgtctggggc cggcagacat cctggagtcc gatgagaatg gtattattcc
      181 ggagcaggac agggtaatca cgcaggtggt gatactggat gcggataaaa agcagataca
      241 gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg
      301 gatgaagtaa cccgacagct tcacaaaacc ggagtccggc tccggttttt gtgttgcaat
      361 gtccggggga tatttgttag gtagattgta ttgtatgtaa aggcagagct ccgaatttta
      421 ctgttgtaag tgggcctggc gggaatggta tcgccagcat cattggcttg ggtccagtaa
      481 aaagagacgc agaaagatgc acaataggca ctacgcgtca tgcatggatt aggtcgctca
      541 ttatttacac agtactgcta aaaaattatt aaggaaagcg cggcacttcg tatgtaagag
      601 cgtgtcgcgg ttggctggtg aactttcgat agtgcgagta ttgaatgatt tccagccgtt
      661 accgatttac gtgtttatta gcgaacaaac cactcgtcag cagactccca ggtatctttc
      721 agcgtctcct gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg
      781 ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt
      841 cgggttaatt cttcctttaa cgcatccaca gcaccgtttg gcattttagt gattttttct
      901 ttagcgatac agatctcaac acgcataaca ccctcctgta actgtagtta tgtacaggtg
      961 ttatttttat ctgtatggat aaccagtgtc aattcctgat attgtttatg gggcatcaat
     1021 ggggcatgta tgggacactt tttatcggcg aaattcgtcg aagttcatcg acatggtaaa
     1081 cgaatcatct atccaaccct tgaaaaacgg cgctcctgga cgatcttcgt cgatttttaa
     1141 aaatgttgcg tcacgcgcgt aacgtgacag ggttaatatc acaaagcaac gccacttcac
     1201 caattgtgta aagcgccatc gtctcaccct tgctcgcgag gtcccggttt aactttagac
     1261 gcagttttgc gaaccaggta gttttgcccg ttttttgtgc atttataggg tgattttatt
     1321 tttgccaggc gattttgagt gatcgtactc acgaattctc atttttctgc aagagttcaa
     1381 agaaagttaa acgcaggcaa tgtatgttac gcgttttaaa gggatgtgtg gtttgcgggt
     1441 atgtacgatt ttaatctggt gttgctgctg cttcagcaga tgtgcgtttt tttagtcatt
     1501 gcgtggttaa tgagtaaaac gccattattc ataccgttaa tgcaggtcac ggttcgtctg
     1561 ccgcataaat ttctctgcta catcgtcttt tccatcttct gcatcatggg cacctggttt
     1621 gggttgcaca ttgacgattc tattgccaat acccgtgcga taggcgcggt aatgggcggc
     1681 ttactcggcg gtccggtcgt cggtgggctg gttggcctga ccggcgggtt acatcgatat
     1741 tcgatggggg gcatgaccgc gctaagttgc atgatctcga ccatcgttga aggattgctc
     1801 ggcggcctgg tacacagcat cctgatccgt cgcgggcgca ctgataaagt ctttaacccc
     1861 attaccgctg gtgccgtcac gttcgtcgct gaaatggtgc aaatgctgat catcctggcg
     1921 atcgcccgac cttatgaaga tgcggtgcgt ctggtgagta atattgctgc gccaatgatg
     1981 gtcaccaata ccgtcggcgc ggcgctgttt atgcgtatat tgctcgataa acgcgcgatg
     2041 tttgaaaaat acacttcggc tttttctgcc actgcgctga aagtggcagc ctcgacggaa
     2101 ggcattttgc gccaggggtt taacgaagtg aacagcatga aagtggctca ggtgctgtat
     2161 ctggagctgg atattggtgc agtcgcgatt accgatcgag agaaattgct ggcctttacc
     2221 ggaattggtg acgaccacca tttacccggc aaaccgattt cttcaactta tactttaaaa
     2281 gcgattgaaa ccggtgaagt ggtctacgct gatggcaacg aagtacctta tcgttgctct
     2341 ttgcatccgc aatgcaaact ggggtcgacg ctggtaattc cgttgcgtgg tgaaaatcag
     2401 cgggtgatgg gcaccatcaa attgtatgaa gccaaaaacc gtttatttag ttcaatcaac
     2461 cgcacgctgg gcgaggggat tgcgcaactg ctttcggcgc agatcctcgc cgggcaatat
     2521 gagcggcaaa aagcgatgct cacccagtca gagatcaaac tgcttcacgc ccaggtgaac
     2581 ccccattttt tgtttaatgc gcttaacacc attaaagcgg tgatccgtcg cgacagcgaa
     2641 caggccagcc agctggtgca gtatctttcc acttttttcc gcaaaaactt aaagcggcct
     2701 tcggagtttg ttactctcgc cgacgaaatt gaacatgtga atgcttatct gcaaattgaa
     2761 aaggcgcgct tccagtcgcg gttgcaggtc aacattgcta ttccgcaaga attatcccag
     2821 cagcaattgc ccgcgtttac cctgcaaccg atagtggaaa acgccattaa acatgggaca
     2881 tcacaactgc tggatacagg gcgagtggca atcagcgtcc gacgtgaggg gcaacatttg
     2941 atgctggaga tcgaagacaa tgccggtttg tatcaaccgg taaccaatgc cagtgggctg
     3001 gggatgaatc tggtggataa gcgtttacgt gaacggtttg gcgatgacta tgggataagc
     3061 gtcgcctgtg agcctgatag ttacacccga ataacgttac gactaccatg gagggacgag
     3121 gcatgattaa agtcttaatt gtcgatgatg aaccgttagc acgggagaac ctgcgcgtat
     3181 ttttgcagga gcagagcgat attgaaatcg ttggtgagtg ttcaaacgcc gtggaaggga
     3241 tcggcgcggt gcataaactg cgcccggatg tgctgtttct cgatatccag atgccgcgca
     3301 tcagtggtct ggaaatggtg gggatgctcg acccggaaca tcgtccgtat attgtttttc
     3361 tcaccgcgtt tgacgaatac gctattaaag cctttgaaga acatgccttt gactatctgc
     3421 tgaagccaat tgatgaagcg cgactggaga aaacgctggc gcgattgcgt caggagcgca
     3481 gcaagcagga tgtttcgctg ttaccggaaa atcaacaggc gctgaaattt atcccttgta
     3541 cggggcatag tcggatttat ttgctgcaaa tgaaagatgt ggcatttgtc agcagtcgga
     3601 tgagcggtgt ctacgttacc agccacgaag ggaaagaggg ctttaccgaa ttgacattac
     3661 gtaccctgga aagtcgtaca ccactactgc gctgccatcg tcagtatctg gttaacctcg
     3721 cgcatttaca ggagattcgt ctggaagata acggtcaggc cgagttgatt ttgcgtaatg
     3781 gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt aaaagaggcg attggcctgt
     3841 aaaagactgc taaaatggct ttttgcctca tcaacacctg aaggcctcat gctaagtaac
     3901 gatattctgc gcagcgtgcg ctacattttg aaagccaata ataatgacct ggtgcgtata
     3961 ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg ccgtctggct acgtaaagaa
     4021 gacgaagagg gttttcagcg ttgtccggac attgttttgt cgtcattcct caatggcctg
     4081 atttatgaaa aacgcggcaa ggatgagtct gctccggcac tggagccgga acgtcgcatt
     4141 aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt cgctgaaaac cgatgacatt
     4201 ctggcgatcc tcaccgaaca gcagttccgc gtttcgatgc cggaaattac ggcgatgatg
     4261 cgtgcaccgg atcataaaaa cttccgcgaa tgcggcgatc aatttttacg ttattttctg
     4321 cgtggactgg cagcgcgcca gcatgtgaag aaaagctaag acgggtatgg cggccatgcg
     4381 caaacatggc cgccgacaga ttatttcact tctttaaaac cagcggcttt catcaccagt
     4441 tccatttgcg ccatagtgat accttttttg gcatcttcag cagaaacgtt gattcctgaa
     4501 ataccctgca gggctttaaa atccactttt tccatatcga tagtcacgtt ttcctgcgcg
     4561 taggtatcgg tataggttaa tttttcttca acacccgcga tgtttttgta tttggcgctt
     4621 aacggctcaa gtgtcttggc agcatcttct ttggtggtag caccaatgga ggcaaattga
     4681 attttggttt cagaagattg cttaagcacc ttgtcacctt tgtagacata ggtaatggca
     4741 atttcagtgc cgttcagatt ggcgctgaat ttcttcgatt cttctttgtc accgcagcca
     4801 gcaagagaaa aaaccagaac agatgcaaca acaagggaaa acagcttatt gaaagccttc
     4861 atgtaaaact ccattttatt taatcaagga actggtgact ctcaccaggg gctatatagg
     4921 atatgcctaa tactgtggcg tgagcagtcc ggaactggag tagaactctt agtaaaaagc
     4981 actatt
//
LOCUS       DAZFKY010000258         4936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFKY010000258 DAZFKY010000000
VERSION     DAZFKY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..1433
                     /gene="garP"
                     /locus_tag="ACHKCL_004493"
     CDS             99..1433
                     /gene="garP"
                     /locus_tag="ACHKCL_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5765317.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1449..2219
                     /gene="garL"
                     /locus_tag="ACHKCL_004494"
     CDS             1449..2219
                     /gene="garL"
                     /locus_tag="ACHKCL_004494"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5765318.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2249..3139
                     /gene="garR"
                     /locus_tag="ACHKCL_004495"
     CDS             2249..3139
                     /gene="garR"
                     /locus_tag="ACHKCL_004495"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5765319.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3236..4381
                     /gene="garK"
                     /locus_tag="ACHKCL_004496"
     CDS             3236..4381
                     /gene="garK"
                     /locus_tag="ACHKCL_004496"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5765320.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4414..4790
                     /gene="rnpB"
                     /locus_tag="ACHKCL_004497"
     ncRNA           4414..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKCL_004497"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1159 a         1188 c         1410 g         1179 t
ORIGIN      
        1 aattcccgag ttcattgtcg ttacggcata aatgaaactc ataaataaca tcttccccat
       61 aataataaaa atcagcataa gtacccgagg taaataaaat gattctggac accgttgacg
      121 aaaaaaagaa aggcgtgcat acccgctatt taatattact gattattttt attgttaccg
      181 ccgttaacta cgccgatcgt gcaacgctgt ctattgctgg taccgaagtg gcaaaagagt
      241 tgcagttaag tgcggtttcg atgggttaca tcttttctgc ttttggctgg gcctacttgc
      301 tgatgcaaat ccccggcggc tggctgcttg ataagtttgg ctcgaaaaaa gtttacacct
      361 acagcctctt tttctggtcg ctattcacct tcctgcaagg ctttgttgat atgttcccgc
      421 tggcctgggc agggatctcc atgttcttta tgcgctttat gctcggcttc tcggaagcgc
      481 catcattccc ggcgaacgcc cgtattgtcg ccgcctggtt cccgacgaaa gaacgtggta
      541 ctgcctccgc catctttaac tcggcgcaat atttctcgct ggcgctcttt tcgcctctgc
      601 ttggctggct gactttcgcc tggggctggg agcacgtctt taccgttatg ggggtgattg
      661 gttttgtgct gacggcgctg tggatcaaat tgattcataa cccgacagat cacccacgta
      721 tgtctgcgga agagctgaag tttatctctg aaaatggcgc ggtggtcgat atggaccaca
      781 aaaagccggg cagtgcggca gcaagcggac ccaaactgca ttacatcaag caattgctct
      841 ctaaccgcat gatgttgggc gtatttttcg gacaatattt tatcaacacc atcacctggt
      901 tcttcctcac ctggttcccg atttatctgg tgcaggaaaa aggtatgtcg attctgaaag
      961 tgggtctggt cgcctcgatt ccagcactgt gtggttttgc gggcggcgtg ctgggaggtg
     1021 tcttctcgga ttatctgatc aaacgcggtt tatccctgac cctggcacgt aagctaccga
     1081 ttgtgctggg aatgttgctg gcttccacca tcatcttatg taactacacc aacaacacca
     1141 cgctggtggt catgctgatg gcgctggctt tctttggtaa aggatttggt gcgctgggct
     1201 ggccggtgat ttctgacacc gcgcctaaag agattgttgg cctctgcggc ggcgtcttta
     1261 acgtctttgg caatgttgcc tccattgtca ctccactggt gattggctac ctggtaagtg
     1321 aactgcactc cttcaatgca gcactggttt tcgtgggatg ttcagcgcta atggcgatgg
     1381 tctgctacct cttcgtagtt ggcgacatta aacgtatgga attgcagaaa taagcaaagg
     1441 tataagcgat gaataacgat gttttcccga ataaattcaa agccgcactg gctgcgaaac
     1501 aggtacaaat tggttgctgg tcagcactct ctaacccgat tagcactgaa gttcttggtt
     1561 tggctgggtt tgactggctg gtgctggatg gcgaacatgc gccaaacgat atttcaacgt
     1621 ttattccgca gttaatggcc ctgaaaggca gcgccagcgc gccagtagtg cgagtgccga
     1681 ccaacgagcc ggtaattatt aagcgtcttc tggatatcgg tttctataac ttcctgattc
     1741 cttttgtaga aacaaaagag gaagcagagc aggcggtggc atcgacgcgt tatccgccag
     1801 aaggcattcg cggcgtctcc gtttctcacc gcgccaatat gtttggcacc gtggcggatt
     1861 atttcgctca gtcgaacaag aacatcacta ttctggtcca gatagaaagt cagcagggcg
     1921 tagataacgt cgatgctatt gccgctaccg aaggcgtaga cggcatcttc gtcggcccca
     1981 gcgatctggc cgcggcatta ggccatctcg gcaatgcatc acacccggat gtacaaaaag
     2041 caattcagca catttttaac cgtgccagcg cgcacggcaa acccagcggt atcctcgcgc
     2101 cggtcgaagc cgatgcgcgt cgttatctgg aatggggcgc gacgtttgtg gctgtcggca
     2161 gcgatctcgg cgtcttccgc tctgccactc agaaactggc tgataccttt aaaaaataac
     2221 caccaccgca acaagagaga tgattgatat gactatgaaa gttggtttta ttggcctggg
     2281 gattatgggt aaaccaatga gtaaaaacct tctgaaagca ggttactcgc tggtggttgc
     2341 tgaccgtaac ccagaagcta ttgctgacgt gattgctgca ggtgcagaaa cagcttctac
     2401 ggctaaagcg atcgctgaac agtgcgacgt catcataacc atgctgccaa actcccctca
     2461 tgtgaaagag gtggcgctgg gtgagaatgg cattattgaa ggcgcgaagc caggtacggt
     2521 attgatcgat atgagttcta tcgcaccgct ggcaagccgt gaaatcagcg aagcgctgaa
     2581 agcgaaaggc attgatatgc tggatgctcc ggtgagcggc ggtgaaccga aagccatcga
     2641 cggtacgctg tcagtgatgg taggcggcga caaggctatt ttcgacaaat actatgattt
     2701 gatgaaagcg atggcgggtt ccgtggtgca taccggggaa atcggtgcag gtaacgtcac
     2761 caaactggca aatcaggtca ttgtggcgct gaatattgcc gcgatgtcag aagcgttaac
     2821 gctggcaact aaagcgggcg ttaacccgga cctggtttat caggcaattc gcggtggact
     2881 ggcgggcagt accgtgctgg atgccaaagc gccgatggtg atggaccgca acttcaagcc
     2941 gggcttccgt attgatctgc atattaagga tctggcgaat gcgctggata cttctcacgg
     3001 cgtcggcgca caactgccgc tcacagctgc ggttatggag atgatgcagg cactgcgtgc
     3061 agatggttta ggaacggcgg atcatagcgc cctggcgtgc tactacgaaa aactggcgaa
     3121 agtcgaagtt actcgttaat gacgtcgtgc ccggatggcg tattgcaatc cgggcctgga
     3181 atccaggccg aataagagaa acgccctccg gcgtcatgtg gtaacaggca taggtatgaa
     3241 aatcgtaatc gccccagact cttataaaga aagtttatct gccagcgagg ttgcgcaggc
     3301 gatagaaaaa ggatttcggg aaatttttcc tgatgcacag tacgtttctg ttcctgttgc
     3361 cgacggtggc gaaggaacgg tggaagcgat gattgcagcc acccagggtt ccgaacgtca
     3421 cgcctgggtt acagggccgc tgggcgagaa agtgaatgcc agttggggga tctccggcga
     3481 tggcaaaacc gcgtttattg aaatggcggc ggccagtggg ctggagctgg tacctgcgga
     3541 aaaacgcgat ccactcgtga ccacttcacg cggcacaggc gagttaatcc tgcaggcgct
     3601 ggagagcggc gcgacaaaca ttattatcgg cattggcggc agcgctacaa atgatggcgg
     3661 cgcaggcatg gtacaggcgc tgggggcgaa attatgcgac gccaacggca atgaaattgg
     3721 ttttggcggc ggtagtctta atactctgaa tgatattgat atttccggcc tcgatccgcg
     3781 cttaaaagat tgcgtcattc gcgtcgcttg tgatgtcacc aatccgctgg tgggcgataa
     3841 cggcgcatcg cgcatctttg gcccacaaaa gggagccagt gaagcgatga ttgttgagct
     3901 ggacaataac ctctctcact atgccgaggt cattaaaaaa gcgctgcatg ttgatgtgaa
     3961 agatgtcccc ggcgcaggag ctgcgggtgg tatgggcgcg gcgctaatgg cgtttcttgg
     4021 tgcggaactg aaaagtggta ttgaaatcgt cactacggcg ctgaatctgg aggaacatat
     4081 tcacgattgt acgctggtga tcaccggtga agggcgtatt gacagccaga gtattcacgg
     4141 caaggtaccg attggtgtcg caaacgtggc gaagaagtac cataaaccgg tgattggcat
     4201 tgcgggtagc ctgaccgatg atgttggcgt tgtacatcag catggcattg atgcggtctt
     4261 cagcgtattg accagcatag gtacgttgga cgaagcattc cgcggggctt atgacaatat
     4321 ctgccgtgct tcacgtaata tcgccgcgac actggcgatt ggaatgcgca acgcggggtg
     4381 acaagggcgc gcaaaccctc tatactgcgc gccgaagctg accagacagt cgccgcttcg
     4441 tcgtcgtcct cttcggggga gacgggcgga ggggaggaaa gtccgggctc catagggcag
     4501 ggtgccaggt aacgcctggg ggggaaaccc acgaccagtg caacagagag caaaccgccg
     4561 atggcccgcg caagcgggat caggtaaggg tgaaagggtg cggtaagagc gcaccgcgcg
     4621 gctggtaaca gtccgtggca cggtaaactc cacccggagc aaggccaaat aggggttcat
     4681 aaggtacggc ccgtactgaa cccgggtagg ctgcttgagc cagtgagcga ttgctggcct
     4741 agatgaatga ctgtccacga cagaacccgg cttatcggtc agtttcacct gatttacgta
     4801 aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg actgtccacg
     4861 acactatacc caaaagaaag cggcttatcg gtcagtttca cctgatttac gtaaaaaccc
     4921 gcttcggcgg gttttt
//
LOCUS       DAZFKY010000259         4932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFKY010000259 DAZFKY010000000
VERSION     DAZFKY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(48..809)
                     /gene="kduD"
                     /locus_tag="ACHKCL_004498"
     CDS             complement(48..809)
                     /gene="kduD"
                     /locus_tag="ACHKCL_004498"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5765321.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(839..1675)
                     /gene="kduI"
                     /locus_tag="ACHKCL_004499"
     CDS             complement(839..1675)
                     /gene="kduI"
                     /locus_tag="ACHKCL_004499"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5765322.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1962..3143)
                     /gene="yqeF"
                     /locus_tag="ACHKCL_004500"
     CDS             complement(1962..3143)
                     /gene="yqeF"
                     /locus_tag="ACHKCL_004500"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5765323.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3398..4627
                     /gene="yqeG"
                     /locus_tag="ACHKCL_004501"
     CDS             3398..4627
                     /gene="yqeG"
                     /locus_tag="ACHKCL_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5765324.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1299 a         1253 c         1064 g         1316 t
ORIGIN      
        1 taaaaaaccc tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc
       61 caaccgccat ccacagcaat ggtataacca tttacataat ctgaagcgct ggaggcaaga
      121 aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcggtcg
      181 agaatttccg cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg
      241 taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcatt cgccatcaat
      301 cgcgtcacgc ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc
      361 tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg cgcgatgaag
      421 tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg
      481 tcccagtcct tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc ggcgttattc
      541 accaggatat caatatgacc aaactcagct accgcgcgat ccagcagtcc aggaatacca
      601 tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc
      661 ttgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc
      721 agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta
      781 ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact
      841 agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca
      901 tgccccagat aaaggtataa gctttggttc cgacgccgga atgaatcgac cagctcgggg
      961 agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca
     1021 tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct
     1081 cgtgggtgtg acacggcatg gtgttccaca agttacccgg agccagctcc gtcagcccca
     1141 tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatc gtgcgacggt
     1201 tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg
     1261 taggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtgccggtat
     1321 caatactagc aaagacaact tcttttgcgc ctttacccac atacagggcg tcgcggtgac
     1381 cgatttcata gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac
     1441 ctaactcgcg acgttcaagg aaataactta cgccgagttg tttacccact tccccgccaa
     1501 cggaaaccgt tttagttacc ggcataatgc cgccaataat aatacggtca atgtggctgt
     1561 aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc
     1621 cctgggtttc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg tccacatcaa
     1681 cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc
     1741 tctataaggg gatgatatgc ccatgggatc gggttttcaa ttaaaaataa aacaatgttt
     1801 catttttaag ttaaggatta aaaaaagtgc agtgtcgatc acgaatctgt catgagaatg
     1861 gaaaaatcag ggagcccgga aaatgtatgt cgacttgcca gccatcatta ttcagcagat
     1921 taatgacggc agatatttat cggtaaggag gaaatgcaga gctattcgtc acgttcaatg
     1981 gtcaatgcca caccctggcc cccgccgata caaagcgttg ccagtccttt gcgggcatta
     2041 cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg
     2101 tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc
     2161 ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcaacatca
     2221 gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc
     2281 atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa
     2341 ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg
     2401 gatgatgcat tacccgctgt cacagaaccg agactatcaa atgaaggatt taaacgggct
     2461 aaaccttctg cactggcgtc agtgtgtggc tgttcatcgg tatcaacaac caacgtctga
     2521 ccgttacttt gggtcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc
     2581 gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaattgacg gctgatgcca
     2641 tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccat
     2701 aacccatcat gcacaagact gtcaaccagc tggctattgc caagctgtgc accggtgcgg
     2761 ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata
     2821 tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag cccggaaccg
     2881 cagacgtcat taatcgtgat tgcagaaacg ctattaggca gaccaccttt aatagccgat
     2941 tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc
     3001 accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt
     3061 tccacggcgg aatgacccgc taacgcaccg cgaaagcagc cgataggtgt ccgtaacgcc
     3121 ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg
     3181 attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata
     3241 tacctgagca gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taccccacca
     3301 atagaggggt agatagggcg ttaatctccc atacttaacc tggtttatgg taaattgccc
     3361 tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga
     3421 agaaactctg tggagtttcg cgctctacgg cacagccgtt ggtgcaggca cgctcttcct
     3481 tcctattcag ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg
     3541 gcctttaaca tactggccac ataaagcttt atgccagttc atcctctcat cgaaaacatc
     3601 agcaggtgaa gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct
     3661 gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat
     3721 taccaactca cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat
     3781 gttggtgagt ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc
     3841 cactattcgg gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc
     3901 catttacctt gtaggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca
     3961 gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac
     4021 gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga
     4081 taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt
     4141 tgtctttagc tgcctgcttt ctattccacc ttcgtatatt gaagcggcta aagaagaagg
     4201 ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc
     4261 cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt
     4321 tattgaaggt gccacggagg tcgtcaaaac aacactacag caggttggtg taaagaaaag
     4381 tcgtgcattt aaccgcgcac tatcaattat gttggtatcg ctgattacct tcattgtttg
     4441 ttgcattaac ccgaacgcga tttcgatgat ttatgcgatc agcggcccgc tcattgccat
     4501 gatacttttc atcatgccta cgctatcaac atatctcatc ccggcgctta aaccctggcg
     4561 ctccatcgga aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt
     4621 tagctaatat ccattgtgaa tggttcaaaa ttgctacaat tttgaaccat tccagttttc
     4681 ataatgtgca tttgttatta ttccgctcta tcacttctct cgtcatatcc tagtattatc
     4741 agttagttaa acacacaaag agatctgaac atcttaaata agactattta agatgcataa
     4801 cttagattcg caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca
     4861 taccatgaga tagttatctg aaccaccctc aaccaggtaa gattaaatca gtgaaacata
     4921 atcatattta at
//
LOCUS       DAZFKY010000260         4839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFKY010000260 DAZFKY010000000
VERSION     DAZFKY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            73..786
                     /gene="ydiV"
                     /locus_tag="ACHKCL_004502"
     CDS             73..786
                     /gene="ydiV"
                     /locus_tag="ACHKCL_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5765325.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            849..2285
                     /gene="selO"
                     /locus_tag="ACHKCL_004503"
     CDS             849..2285
                     /gene="selO"
                     /locus_tag="ACHKCL_004503"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5765326.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2289..2480)
                     /gene="hemP"
                     /locus_tag="ACHKCL_004504"
     CDS             complement(2289..2480)
                     /gene="hemP"
                     /locus_tag="ACHKCL_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5765327.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2612..3658)
                     /gene="aroH"
                     /locus_tag="ACHKCL_004505"
     CDS             complement(2612..3658)
                     /gene="aroH"
                     /locus_tag="ACHKCL_004505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5765328.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3815..4648)
                     /gene="ppsR"
                     /locus_tag="ACHKCL_004506"
     CDS             complement(3815..4648)
                     /gene="ppsR"
                     /locus_tag="ACHKCL_004506"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5765329.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1196 a         1131 c         1235 g         1277 t
ORIGIN      
        1 aattgttatg tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga
       61 gggacggtta ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg
      121 ccgataagag ataaccagca agtccttgtc ggcgttgagt taattaccca tttctccagt
      181 gaagatggca cggtgcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa
      241 cattggcaat tattttcaga gcagttggaa ttactaaaat catgccaaca tttttttatt
      301 caacataaac tctttgcctg gcttaattta acgccacaag tggctacatt attattagag
      361 agggataatt acgcaggcga actattaaaa tatccgttta ttgaactgtt gattaatgag
      421 aattacccgc atctcaatga aggaaaagat aatcgcggtc ttttatcctt atcgcaagtg
      481 tatccccttg tgttaggtaa tttgggagca ggcaacagca caatgaaagc ggtttttgat
      541 ggtcttttca cccgcgtaat gctggataaa agttttattc agcaacaaat cacgcatcgc
      601 tccttcgaac cgtttattcg tgcgattcag gcgcaaattt ccccttgttg caattgcatc
      661 atcgcggggg gcattgatac gccagaaata ttagcacaga ttaccccctt cgatttccat
      721 gctctccagg gatgcctgtg gcctgccgtt cccataaatc agataacgac gttggttcag
      781 cgataaccct cctgtttgct ggtgtttaag acgagagtaa ccgtctacac tatcaaacag
      841 gaggctctat gaccctgtct tttattaccc gctggcgcga tgaattgcca gaaacctata
      901 cagcactttc ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactcg
      961 ctaacacgct gagtattcca tcgtcgctgt ttaaaaatgg cgcaggtgtc tggggcggcg
     1021 aaaccctact gcctggtatg tcaccactgg cgcaggttta cagtggtcat cagttcggcg
     1081 tctgggcggg ccaactgggt gatgggcgcg gcattttact tggcgaacaa ctgcttgctg
     1141 atggcactac aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg
     1201 gtgatggacg ggcggtatta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc
     1261 attatctggg cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt
     1321 atcgggaaac ggtggagcca ggcgcgatgc tgatgcgtgt ggcaccaagt catctgcgct
     1381 ttggtcattt cgaacatttt tactatcgcc gcgagccgga taaggttcgt cagttggctg
     1441 actttgccat tcgtcattac tggtcacatc ttgaagatga tgaggacaaa taccgtctct
     1501 ggtttaacga tgttgtcgca cgtaccgcat cgttaattgc ccaatggcag acggtcggct
     1561 ttgcacatgg ggtgatgaat accgacaaca tgtcgctgct gggactgacg cttgattacg
     1621 ggccgtttgg ttttcttaat gattacgaac ccggttttat ttgtaatcac tcggatcatc
     1681 aaggacgtta cagctttgac aatcaacctg ctgtcgcgtt gtggatttta cagcgtctgg
     1741 cgcagacatt gtcaccattt gttgccgtag atgccctgaa tgaggccctg gacagctatc
     1801 agcaggtttt gttgacgcat tatggacaac ggatgcggca gaaactgggc tttatgacgg
     1861 agcaaaaaga ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca
     1921 gcgattatac ccgcacattt cgcatgctga gtctgaccga gcagcacagc gcggcgtcac
     1981 cgctacgtga tgagtttatt gatcgtgcgg catttgatga ctggtttgcc cgttatcgag
     2041 ggcgtttgca acaagacgag gttagcgata gtgagcgtca gcaactgatg caaagcgtta
     2101 accccgctct ggtattgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg
     2161 gtgatatgac ggaattgcac cgcctgcatg aggcgttgcg aaatcctttc agcgacagag
     2221 atgatgacta tgtcagtcgt ccacctgact ggggtaaacg gctggaagtc agctgctcga
     2281 gttaaaggct acttggtcaa cagcagcttg ccagcctgag ttttacggag cagatattct
     2341 tgcccgtcat gatcgataat cagtttgcca tccggaccta acagtgtctg gctggaaatt
     2401 cgccgaatag gctgcgggga agcggtcttg tgattggcat ccgtttcagg cgtgagtttt
     2461 ctgctatccg tataacgcat aaaaattaat aataattgat ataacaatga ttcttattat
     2521 caatattatg gctgaccaga gcaagcagta atgtgttgag aagacaacaa gttgcgcaga
     2581 aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg ggtatctacc gcagaggcta
     2641 gtttttcgac caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac
     2701 cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg
     2761 ccataattcc agcaatcgcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa
     2821 cttctaactg gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat
     2881 gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat
     2941 aattcggttt tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat
     3001 agatggtcat ctgaccattt ttgtctggcg agaggaacat atggctggcg cgggctgcgc
     3061 ggatagcatc cacagcaatc cgcgtattgc catcggtacc atttttaaaa cctaccggac
     3121 aggagagtgc cgaagccatt tcacggtgga tctgactttc ggtagtacgt gcgccaatcg
     3181 cgccccaact gattaaatca gcaataaact gaccggtcac catatcgagg aactcagtcg
     3241 cggttgggac gcccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt
     3301 gatttatccg atagctgccg tttaaatctg gatcggagat tagtcctttc cagccgacaa
     3361 cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt
     3421 tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg tggatcgagc
     3481 aggggccaat aatgaccaac agtcgcttat cttcaccatt gagtattttt tcaattctgc
     3541 ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt
     3601 cggcgggcgt taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta
     3661 caagtctcag aaatttgttg cttctacggc aggactgccg ggaagtgatg cgcatcacga
     3721 taaaccaatc gctgctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca
     3781 tgataacaaa aaaataagct aatgcactag ttctctagta cattcggcga ctaaggccca
     3841 tgatatcgag gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg
     3901 ggatctgatt tttacggtac aaggcttcca cttccgcgac ttccatcctg cactgacgaa
     3961 gcgaggcata gcgactgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt
     4021 cgatagtcag gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcaccagat
     4081 tatccatatc gtcggcaata aaggggtagt ttgccgcgcg gataccaaat tgcattgcca
     4141 gatacagact ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct
     4201 gatccagatt gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca atcgccgcaa
     4261 tgcgcgcatc atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg
     4321 gcgttggatc cagtttcatc tcttgttgta gcggggcaac cagcgcctga acgatatcct
     4381 ggcaaaaacc ttcactttgc aagatgatgg cgcgaatttc cggcaacacg atggagtaga
     4441 agaccagcgg acgcacgcct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc
     4501 gtgcacggct ctcattttcg acaaacggca gcgtgatgct gctgatagtg acgggaaatt
     4561 gtgacattac tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa
     4621 aaacgtggcg atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta
     4681 tgaaagcata aattaaaaac gcacagaagc gtagaacgtt atgtctggtt tataaaatga
     4741 accttcaatt ttatttttta tgaaaacagc atttcatttt tatggtttcg tttataccga
     4801 tggtttatgt ggaaattgtc gaaaagagca gatttgcgc
//
LOCUS       DAZFKY010000261         4827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFKY010000261 DAZFKY010000000
VERSION     DAZFKY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..342
                     /gene="bor"
                     /locus_tag="ACHKCL_004507"
     CDS             49..342
                     /gene="bor"
                     /locus_tag="ACHKCL_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO5765330.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(433..615)
                     /gene="rzoD"
                     /locus_tag="ACHKCL_004508"
     CDS             complement(433..615)
                     /gene="rzoD"
                     /locus_tag="ACHKCL_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO5765331.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(832..1308)
                     /locus_tag="ACHKCL_004509"
     CDS             complement(832..1308)
                     /locus_tag="ACHKCL_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5765332.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(1295..1600)
                     /locus_tag="ACHKCL_004510"
     CDS             complement(1295..1600)
                     /locus_tag="ACHKCL_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5765333.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1922..2611)
                     /locus_tag="ACHKCL_004511"
     CDS             complement(1922..2611)
                     /locus_tag="ACHKCL_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5765334.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(2608..2748)
                     /locus_tag="ACHKCL_004512"
     CDS             complement(2608..2748)
                     /locus_tag="ACHKCL_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5765335.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2745..3107)
                     /gene="rusA"
                     /locus_tag="ACHKCL_004513"
     CDS             complement(2745..3107)
                     /gene="rusA"
                     /locus_tag="ACHKCL_004513"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5765336.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(3104..3394)
                     /gene="ybcO"
                     /locus_tag="ACHKCL_004514"
     CDS             complement(3104..3394)
                     /gene="ybcO"
                     /locus_tag="ACHKCL_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5765337.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3387..3557)
                     /gene="ninE"
                     /locus_tag="ACHKCL_004515"
     CDS             complement(3387..3557)
                     /gene="ninE"
                     /locus_tag="ACHKCL_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5765338.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3557..4012)
                     /gene="ybcN"
                     /locus_tag="ACHKCL_004516"
     CDS             complement(3557..4012)
                     /gene="ybcN"
                     /locus_tag="ACHKCL_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFO5765339.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(4009..4107)
                     /locus_tag="ACHKCL_004517"
     CDS             complement(4009..4107)
                     /locus_tag="ACHKCL_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765340.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            complement(4514..>4827)
                     /locus_tag="ACHKCL_004518"
     CDS             complement(4514..>4827)
                     /locus_tag="ACHKCL_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5765341.1"
                     /translation="EKIRLLDKELRRALVSLKTLKSKGVNSFSDFYAIDLTSKNGREL
                     CRTLAYKIFEKIIINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDCEVY
                     F"
BASE COUNT         1226 a         1191 c         1091 g         1319 t
ORIGIN      
        1 tctacacata cgattctgcg aactttaaaa agcatcggga ataacaccat gaaaaaaatg
       61 ctactcgcta ctgcgctggc cctgcttatt acaggatgtg cccaacagac gtttactgtt
      121 caaaacaaac cggcagcagt aacaccaaag gaaaccatca cccaccattt cttcgtctct
      181 ggaattgggc agaagaaaac tgtcgatgca gctaaaattt gtggcggcgc agaaaatgtt
      241 gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat tactttaggc
      301 atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga gctgcccatc
      361 gatatgggca gctctatctg cactgctcat taatatactt ctgggttcct tccagttgtt
      421 tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg gtgtctgcca
      481 gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc actgactgac
      541 agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcatgc agagcatcat
      601 tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca gcaacatcac
      661 gctgacgcat ctgcatgtca gtaattgcca cgttcgccag cttcagttct ctggcatttt
      721 tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca gcccatgaca
      781 ggcagacgat gatgcagatg agcagagcgg agataatcgc ggttattctg ctcatacctc
      841 actctctctg accgttccgc cagcttcttt gaattttgca atcaggctgt cagccttatg
      901 ctcgaactga ccataaccag cgcccggcag tgaagcccag atattactgc aacggtcgat
      961 tgcctgacgg atatcaccgc gatcaatcat aggtaaagca ccacgctctt taatctgctg
     1021 caatgccact gcgtcctggc ttttggggga gaagtctttc aaaccaagct gtttacggta
     1081 agcatcccac cagcgtgaaa gaagctgata acgtccggcg gctgttgatt tgagtttggg
     1141 gtgtagcgtg acaagtttgc gagggtgatc ggagtaatca gtaaacagtt cgccgccaac
     1201 aataacatca taaccgtggt tacgtgtcgg ttgtcgcccg ttatccgttc cttctgacca
     1261 cgccaacata tcgaggaagg ctttacgctg aggattaaga ttttgcattt ttcacccctg
     1321 tcagtcgttc ccagaagtac gtcagtgcaa ccgaacccat cgcaccacta atccccgctg
     1381 tcgcgagaat catgtaaata ctgaatccac tttcgatgct gatcaggcca ccaataacac
     1441 cggtgaatcc tgataccact atttgagcca gagcatttat ccaactccac gttgctttac
     1501 tctgcttcac atctatcagg tagcggacca gaccgcccca acctgcgatg atcagcaaaa
     1561 cgagccagaa cgctccggca aggctctctt tttcgtgcat atgaatagcc aatgtttcgc
     1621 cgccgacaaa aggccgggac gttaaatgtc agaaatcagg ctcacggggt aatttaacga
     1681 caaagcacgg agttgatgct ccccacaagc ctggaataaa aaagccagca tgtagctggc
     1741 aacagagggc taagcaatat caactcaaca gctgaagacc ccctggctgg ggtaggttgg
     1801 aaggctactc accgttcaga aacagaaaag cccaaggctt taaacctcgg gcttgaattt
     1861 ggattactgc cagtgcgtac aacattggca aaatatcaga tttacataaa atatatgatt
     1921 tttaatccag ttttgcaata tcttgctgtg aaaatgtggt cttttgtttt gaacgtgttt
     1981 tcgttaaaag caataaagct tggctatcaa gctgtagaaa aatgtgtttc attgcaaccc
     2041 agcgttcagt aaatgtctca gaccagtttt ttgatgtcac tcccaccagt gatgccagct
     2101 cctggtattc ataggtctta cgccctgcca gctcgttctt cacatcctgt gccgccagcc
     2161 agatcaactt cttcaaacgt tccagtgtct tacctgcaat tttcctggta cccaacagag
     2221 tcttaaactc gctccatgcc cactgcgtta tggtgacctg atgttcccag cgaacacttt
     2281 cgctgtaact ccacagcaac cacgctttct gatgttcttc gagagacaga accgcgcggc
     2341 gccatgacga ggttgagaac tcaacctggc tgaccagtgc aatggatgaa ccttttgcgt
     2401 acgactgctt accggaaatc ggcggattat ccagcgtaat catcctgcca gttacctcat
     2461 ccagaatgcg cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctccagcc
     2521 aggcttcaag ctgtcctttc gttgctccgc tcaaatcagc ggtagccaca atgagttgct
     2581 cgcggacata ctgtaaatat tgggtattca tgcggcagct cctttcagtg ttttggcgta
     2641 attcttcagt atccggtaat cggtcaaaac agaaccagga aaacgatata agcgcaggcg
     2701 catccagcgg tggcgaagac gttctgccat ataagactca aacatcattc attccccagt
     2761 tcggtgatgg tcagctccag cttcccacct ttggtaacgg gcatcttcac aacacggtaa
     2821 tcaacgacct gagcatcatc cagccagaaa cctgctttgg tgagtgcgtc aaaagcggct
     2881 ttttgcagat tatccaggtc acggcgacgg cgatccggca tgtggcactc aatacggatt
     2941 ttcactggca tagccaggcc gatatccagc atggagcctt tgatgattcg ggcgacgtta
     3001 tcacggtatg cctgcccttc tgcgctgatg tgcgtgcgcc cgcgattatg ccggtagtag
     3061 cgattattgc tcggaggcca gggtaatgtg atgctgtagg tattcacgcc ttaataaccc
     3121 cctctttcag ccagataacc tgtgttctcg ccataccttc cagcgcgcat tcttttgcat
     3181 atgcagcatc gacaaaatgt gtgcggcggt cgatttcgtc gtggcaggca gaacatgcaa
     3241 tggtggcaat caagtctggc ggtttggtac cggtgccgca caatccagtc agccggatat
     3301 gtgccagtac agacgtttca gggttgccat tacatacgcc agggattctt acttggcatt
     3361 cccgactacg cgctgctttt ctcaaatcag ccatgattcc tccttgctgc cagtcgcaac
     3421 cattttttat caaccaggct ggcggtatat ccgagcagtg ttggtatttc ggatggcttc
     3481 agctcaggtt tacgcttacg acgatttggt actttgtaga tgtgtccgtt catgacacga
     3541 ataagcggtg tagccattac gcctcctgct tgtcacgcag cagctggaac tcgcagctct
     3601 gtggaatagt caggtggcag ccaatattca tcgcccaggc ttcaacctta cacaggaaga
     3661 catacatctc tccggtacca agatcggagg tatggcgtaa cgactggata gtagtgattt
     3721 cgccggttac gacatcaacc aggtccttgg tttcataacc gaggtatgtg tgtttgagag
     3781 catcttttac ccatgctgaa gtagcgaacg atttccccct gctgatgagg tattcactga
     3841 tttcgctgta ccacatgtgg ctgagtgcat tctgggaaag actgcgtctc tcgcgccacg
     3901 gtttaagcac catgcgaaag catttgccgt cttccagata agtctggatc tgctgaccga
     3961 cagcggtgaa gttaccgcga tgtaatttga tgccatcttg tgggaggttc acgcttcacc
     4021 tccgcagaga tcaaacgctg gatgcaagac atcgcaggtg catttctgca tctgtgacaa
     4081 ggtaaggagt tcagattgtg gtcgcattta agtcccctta aatgcgcaga agtcaccgga
     4141 gttgttcaag ctccggtgac attattatgg aaggttgata ctaaaaaatc aaaaattgaa
     4201 tatcattggc gatagtctag cttgtgatat ccacaagcta cttcaatgaa gcgacgagta
     4261 acattttgtg tcatccatgt tttttcagtt aagtgccaca cccagttcaa aatttgctga
     4321 tgtgttgaac atgtactaag cgggatgttg tattcataag caacattgat tccgcaaaat
     4381 cggtgtcgtg tcatgaccga ttcacgttga cctgcgccct gttgagtgcc tggggatatt
     4441 cttgctcgat agttaatatc atagtagtta ctttactttc ataccatagc acggttgaaa
     4501 aagtgattat tactcaaaaa taaacctcac aatcaaccat atatttgaga gcacttatcg
     4561 cctgctgggc ggatattact ttcattaaag gatagtgttt aaaaacaatg ccattcataa
     4621 aatagatatc acaggtttta ttatccgtat taattatgat tttttcgaat attttatagg
     4681 caagtgtacg gcataactct cgtccatttt tactggttaa gtcaatagca taaaaatcac
     4741 tgaatgaatt tacaccttta ctcttcaaag ttttcaatga taccaaagct cttcgtaatt
     4801 ccttatctaa tagtcttatt ttctctg
//
LOCUS       DAZFKY010000262         4736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFKY010000262 DAZFKY010000000
VERSION     DAZFKY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..591)
                     /locus_tag="ACHKCL_004519"
     CDS             complement(<1..591)
                     /locus_tag="ACHKCL_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765342.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVL"
     gene            complement(597..875)
                     /locus_tag="ACHKCL_004520"
     CDS             complement(597..875)
                     /locus_tag="ACHKCL_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO5765343.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(890..2158)
                     /locus_tag="ACHKCL_004521"
     CDS             complement(890..2158)
                     /locus_tag="ACHKCL_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765344.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2155..3780)
                     /locus_tag="ACHKCL_004522"
     CDS             complement(2155..3780)
                     /locus_tag="ACHKCL_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765345.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGA
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4075..4263)
                     /locus_tag="ACHKCL_004523"
     CDS             complement(4075..4263)
                     /locus_tag="ACHKCL_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765346.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            complement(4403..4642)
                     /locus_tag="ACHKCL_004524"
     CDS             complement(4403..4642)
                     /locus_tag="ACHKCL_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5765347.1"
                     /translation="MCGCGKHDDCENGQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT         1214 a         1335 c          950 g         1237 t
ORIGIN      
        1 caggacctta tctatatacg gctttcgaat atcccatgtt gcaccaccgg aataatgctc
       61 acaggaatat attttatttc ctgtagcacc agtggatgtg gtataaatcg gtcgatcata
      121 aggagttctt ttccagttat aatacccgac cagtgcaggc atgattgcgc atggatatcc
      181 aaggtccttt tcaaatttga tatcaacagg tattaacttc gcgtcaagca gcatcatttc
      241 gccatgataa atcatctcac cgtccgggtt atacatggcg ataccatact cagaaggtgg
      301 agtaaccata ttcgcgaaag cataaaccgt cgtaacacca gggttcgttc ccctgacaat
      361 tacatgaagc cttagtgcat gatatccatc aatctgttca tgcgtgtaca tgacatcggc
      421 cttcttctct gttctgataa agaaaaaaca acttttgcct gacgggattg atgttttaaa
      481 aaaagacttt tcagtcgccg gtatggtgcc tttgttgatc agacactgcg gcgtaaaacc
      541 tggacttatc catacgctgc catccggctt cataatgctc aaaccgtaca taacacttat
      601 ccccagaatg tataaatata agatcccata ccctgctcaa gattcgacca cgtcaccgta
      661 ttgtcattaa tggttatctt cggtactttc cgatccgcaa atacattatt ccagggaaat
      721 aaacaacata cagcctgcaa tgatttcccg tcgggtttat tagtgtacgt ctttgagcca
      781 gactccgctg taaatctgtc caggaaaaat actggagtca gcacgcccgt aacattaacg
      841 ttatttctgt tataaatggc aaaaccgtat tccaatactc acctcctgat cagcgtaatc
      901 tgcctatgcg aacagccagt cgtccattct ggtcataaac ctcaatttta tcattgcgga
      961 tcaccagtcc tacattctga ttagagtaac gaattgtcag ttgcccttgt gacgtaacac
     1021 tgaaaaggcc tccaatattc aggttaccct gagaatcaac ctgaaagttt ccgttctgaa
     1081 taacggcact ccggataact ggcgaagtga tacttacccc ggcttttacc tcatccgcca
     1141 caaccttccg cgacaccagt gtttcaatca ccgcgtcata aatcatcgct ttcgggatca
     1201 ctaccttgcc acctgatacc gcaaacggat aggctgtgtt atccggatta ttcgggtcaa
     1261 agacaaacaa ctgcgacgca gaaattgcaa cctgacttac aggcctgcct tcactgtctt
     1321 ttccggcgac aatcccgatc cccgcagtga taccatcaac tcccgctttt tttgaccaca
     1381 ttgcaagaaa agcttcaccg ccttctttat ccaatttagt gatgcgcttg tcgacctcat
     1441 taagcgattc actggttgac gaatccagtg tgctaatgcg ggtttcaata ccaccaatcg
     1501 ttcttgtcgt ttcttccctg agagtcccta caacttcggt tgtcttaatt gcagcatcct
     1561 ttacagcctg cccctgcgcg ttttttattt ctttacgcag ctcggacaca accggcgact
     1621 ttgcagcctc atcgcggatc tggtcaatga tggccttcac gccgatctgt gtttgtgcct
     1681 gagtgccttt ttcagcattc catggacctt tcactcctgc cgcgttaaca aaacgtatcc
     1741 agtaaaatcc cgaccagcca gggtcaaccg gatcgccgta aacctgcccc ggcgtcgtgg
     1801 caaccagcac tgcatcagca aggtcatcct ccgtaccccg ccagatttca gtcagtgaat
     1861 gtccgcgata attaggcata tcccattcaa gaagaaccga gccaaatcca ccggtcgcct
     1921 taaaattcag cggttttgtg ggaaaatcaa cagtcattaa agtactgtca atctcaatac
     1981 ccggattcag tgcatatgag gcaccacccg atgttcgacg ccgggcgagt ttaagaccaa
     2041 ccagttcctc acgggtcaca aatgcgtgac gtccgtcacc acgctgcccg gtgccaattt
     2101 ccatattctc cacaacagtg gataaatcct tccccgcacg ccacggtttt ctggtcatac
     2161 cggcatctcc gacattgatg tactcagggt tattcgttcc acctgcccga atccggatac
     2221 catcacctgc cagttttgcc cggttgctgc cggaagtctc accacacttc ccttaaacgt
     2281 acccggcgca aaatgaatca caggaacatc atcagccata atggtgatcc ccacacgctc
     2341 aggcgccgga gatttcaccc tgatacagga aaaagaggtt ctttcaggta atgaaaaaat
     2401 ttttgaatgc caccttatcg tggagggcag agcccccccg gcaagcactg acattttgtc
     2461 tcctgtcacc acgcgcatca tatctttcgc gagatcaacc catgcgcagt caaacggtgt
     2521 actgagataa cggatatcca tgttcaccgg actgaataca aacacatcct gcttaccatc
     2581 cggtttcgtg taacaggcaa tgtattcacc acgccaggga taagccacaa tggaggccgg
     2641 attaaactga ctctgccact gttccggtga aacaatctgt tccgtcgcca gcgcgacatt
     2701 accgtttgca tcaacagaca ccaggccatt tgttcctgca tacagcacaa aaccctccat
     2761 cgcaaccata ctccgcctgc tcagacacgc ctgcattgaa gggattttgg aaccagaaat
     2821 tgtggacggt gataccccac tgaacaaata aggctccccc tttgtcgcca ccaccagtga
     2881 cgttcccagc ggacagatag ctacaatatc ttctgccgtc gtgtgacgat tcacttccgg
     2941 ccatgcatac ggcagatacg cttccgaaaa catcacttca ttaccggcaa acccggcggc
     3001 aataccgtta gccatcaggc aaaggcctgt catattctct ggcggcggca ggtaatccca
     3061 tgtcgccagg gaaggcccaa ggtttttcgc cggtattttg tccgtgtaac tgagcacgga
     3121 tgcatccagt tcagccacaa gtaaaaaatc cgcctcccct ccacctgatg cagagcgata
     3181 aatccggcga cgtttaatac tggcattctg caatggcacc ggagccagcg tcagttgtac
     3241 cgcagttccc ggagtacgga gtgttacctc cagagacgcc ggacctggcg gaccttcttc
     3301 accataatct gagacaaagg tttccgtata aaaccgggtt tcatcatcat tcgggttatc
     3361 gtcagaaaca tcaccgccct gctgaacagt acagacagga gctgtcgtcg gcgcggggat
     3421 ccccagacga tacgatgatg tcgggtgatt cccgtcccct tttgtggcaa tagtcgcatc
     3481 cgtcacttta ggaaaacgcc cgtcagtgta gtaaatacgc ccgtgggggt cctgagcgat
     3541 cggactgcgg atcacatcca ccacatccgg ccatgcaaac cagaaatcgt cacggtaatg
     3601 aaaaattgtt tttggcttaa ttgtgaatgt tttctcaacc ccggatatct gacgttctgg
     3661 tgtaataaca ccaaaccgga aatggcagtc ctccgccagt acagcggaat gctcgggcag
     3721 catggatgtc acaacgcgcg gcatcatccc acgcatcgtg gtgatatcga tatagggcat
     3781 agaaattcct ttgactgctt caggcttaaa aatcagggga tttatgatgg aattcgctaa
     3841 aatcgcgatc ggcatcactg aaacaaaatg ccttgcatga cattctgatg atgactaaat
     3901 aacttcattg attattgcaa ttttctttcg gtttggggga gcatattgct cccctttttt
     3961 cactgcatta tcattgccgc tgtagcaacg agaagcatca ctccgcacac gcaagatgtt
     4021 tcacactcac accatacagt ccatttttcg ccattcccgt atacagaaag ctattcactg
     4081 tcttcgtgtg aattcccatt tcactggcaa ggcgacatca tgtacccctt tcaatttttc
     4141 cagattcagc tgaataaaac ggcgtccctg atacatactg cgcattaaat ggcccaactg
     4201 actatcacag attattaacc aggattcttt actatccaga ataatcctgc gattttccgg
     4261 cataaaaaac tcttgaacca gaattacacg gcatccttca ctccatcgta ttaacatgtt
     4321 tattacttca ctctctaata acccccccct atacatgaca acaaaaaccg gagccgggct
     4381 ccggtttttg tgaagctgtc ggctatttca tcccgccaat attttcccac gtcccgtcag
     4441 cacgcaggat ttgcagcggt cttaccacac actgtatctg ctttttatct gcatccagta
     4501 tcaccacctg tgtgattacc ctgtcctgct ccggaataat gccattctca tctgactcca
     4561 gaatgtctgc cggtcccagt cgcagttgtg ctgtaagcga ctgcccgttt tcacagtcat
     4621 catgctttcc gcaaccgcac agactctgca taagctttct caaaatattc atgtcattct
     4681 cctgttctgc ctgtatcact gcccacttca tccagcccct tgacatcctg ccacgg
//
LOCUS       DAZFKY010000263         4698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFKY010000263 DAZFKY010000000
VERSION     DAZFKY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(383..1921)
                     /gene="aldB"
                     /locus_tag="ACHKCL_004525"
     CDS             complement(383..1921)
                     /gene="aldB"
                     /locus_tag="ACHKCL_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5765348.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2029..3324)
                     /locus_tag="ACHKCL_004526"
     CDS             complement(2029..3324)
                     /locus_tag="ACHKCL_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5765349.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3454..4605)
                     /gene="yiaY"
                     /locus_tag="ACHKCL_004527"
     CDS             complement(3454..4605)
                     /gene="yiaY"
                     /locus_tag="ACHKCL_004527"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5765350.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1136 a         1250 c         1138 g         1174 t
ORIGIN      
        1 tggaactatc agccaaacta atgaataact tcagtaaaac atccacaatg cagattaagc
       61 gtattaaacc ttcaataatc taccgtttat tgctgattgt cctcggaata ccaatggtga
      121 tttacggtct ggtttgcccg ctcaccatcg aaacgcgaga ttaaactcct ggcaaaacac
      181 gccccagaaa cgcacttttg ttggcgtagt ctgggttatt gcacacgtag gtttaaaacg
      241 taaattgtaa caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc
      301 tacggtcagg tccccatagg catgataaga cgcgtaagcg tcgcatcagg caattaatac
      361 ccgatgcgac cagcttctta catcagaaca gccccaacgg tttatctgag tagctcacta
      421 gcaggcactt ggtttgctgg taatgctcca gcatcatctt gtgggtttcg cgaccgatac
      481 ctgattgttt gtagccacca aacgccgcat gtgccgggta agcatgataa cagttggtcc
      541 acacgcgccc agcctgtatg ccgcgcccca tcttataggc cagattaccg ttgcggctcc
      601 agacgcccgc gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg
      661 ttttgaaggt agtcaccgcc agcaccgggc caaaaatctc ctcctggaac acgcgcatat
      721 tgttctgacc aaacagaatc gtcggttcga ggtagtagcc gtctttcagt tcaccttcca
      781 gcagcttgcg ccgcccgccg gtgagcacat cagcgccctc ttttttaccg atatcaatgt
      841 agttgaggat ggtttccagt tgcccgtgag aaacctgcgc acccatttgc gtcacgctgt
      901 cgagtgggtt accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt
      961 cgtagattga ctcctgcact aaggcacgac tcggacaggt gcaaacttcg ccctggttaa
     1021 aggcaaacag tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca
     1081 catcagcaaa gaagatattt ggcgacttac cgcccagctc cagcgtcacc ggaataatgt
     1141 tttgcgtggc gtattgcata atttgttgac ccacttccgt tgagccggta aacgccactt
     1201 tggcgatgcg tttcgaggtc gccagatatt cgccaatttc cccacctgca ccgttgacca
     1261 cgttcaccac gcccggcggc agtaaatcac cgacgatttc cattagcagc agtacagaaa
     1321 gcggggtaag acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca
     1381 ttttccagct cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta
     1441 acggttcatg gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct
     1501 gcgcccgaat acacgaggcg aaatagcgga aatggtcaat cgccagcggt acatccgcag
     1561 cactggtttc gcgaatgggt ttgccgttat cccaggcttc agctgtcgct aacagctcga
     1621 ggttttgttc cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg
     1681 tgtgcgccca tttatctttc actttgtgcg cagcatccag cgccagatcg atgtctcgtt
     1741 tgcccgaaga cgccacttcg cacagcagct gcccggtcac cggcgtcagg ttctggtaat
     1801 actcaccatc ggcaggggct acccattcgc cgccaataaa gttgtcatag cggtttttta
     1861 acttgagggg gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca
     1921 tgatcgtctc cttgcggtgt gaggtataac aagggtagac gtggctggcg aaatcttcgc
     1981 cagtcggtaa cagcttttcg acggctatca cgaatttacg ggcaagggtt acagcggata
     2041 atgcgcgaag tacacggctt gctggtcatt aattttcttc tcttgcgcaa acccttcttt
     2101 aacaattgtc cgtaaatcag ctcgaaccgt attatcagag acgcccaggc tcgcgctaat
     2161 ttcagcaact gtgtactctt tccccggatt ggcgagcatc acattcagga gcgttacctg
     2221 gcgcggattc aggcgtttaa gcacaccaga gctaaacagc acactatcga gttttgcgct
     2281 tcgttgtgta atttcatcga tatgctgctg ccagttttgc agagcacgtt taatgacacc
     2341 cgcctgatat tcaaggaaat aagtcagatc cataccatct gattcagtgt actggtagct
     2401 ggcggcatat ttgaccggtg cagcatgaag caaacggctg atggaaatat atttaaagac
     2461 atcgtagccc gatttcagca tataccagta aaacagcgcc cgactggtgc gcccgttgcc
     2521 atcccgaaac ggatgttcat gggccagcat aaaatgcaga atgcaggcac ggaccaacgg
     2581 atgaatatac ccttcatggc tgtttaacca ctggcaaact ttttccagcc gttccggtaa
     2641 caatgccgca gcgggcggtt gatgcactat attgccatca tagtcggcga tcacaacatc
     2701 atctgtttca cgaaattctc ccggacgata tttcgcgtca ttgattccac ccataccaat
     2761 ggcatgtaac tggcgaataa gcgcaggtgt cagcggttct gcgagtaagt gcggaatttc
     2821 cgccatcagc cgggcatttc ccgcaatcat atgctcgtct tcagtgcgtg gcgaccgtcc
     2881 tgtttccagc atttttctcg ccaccagcgt tgtagtattc gcaccttcca gctgggagct
     2941 ggtgatcggc tcttccaggc gtaactggct taactcggct ccggccccgc gtaaccgttc
     3001 agtctgatct ttcagcgcca gcgcggtagc acgtttgtcg acaagctcgc acgcttctgc
     3061 aatcaccggg gtaatgttga atccggcctg ttcacccgct tcattgcaat aattaattcg
     3121 ttggatcgcc gcgttgcgcg ccagacgggt aagcgtccac gccacatcga cgttttctcc
     3181 ttttttgatg cggtactgca actcatcaaa aggcagataa cgtccttttt catcggtaac
     3241 ccggttaaag cgcaggtggg cctggagttc ctcacccgat aagtgtgata aaggaatcag
     3301 cgtgggaggt gatttgatag ccatttgctt atctcaaaaa agcgcaatat tgagatagtg
     3361 tatagcagga aagagaaact atctcaaaaa aagcaagttt tgagatagtt gaaaaaatgg
     3421 ctgacaattt gccagccgtt gtggaaatga tgattacatc gctgcgcgat aaatcgccac
     3481 aatttcttcg tgagttgcct ggatcgggtt agtaaagcca caggcatctt tcagggcatt
     3541 agccgccaga accgcgaaat cttcttcttt cacgttcagg tcgcgtaggc ctgccgggat
     3601 atccactttc ttcgccagtt cacggatggc gttaatgcag gcttcagcac cttccgcgtc
     3661 gtttttacct gtcacgttca cgcccattgc agcggcacag tcacgcagac gtgcagcggc
     3721 gactttgctg ttgaatacct gaacatgcgg cagcagaacg gcgttacata caccgtgtgg
     3781 caggttgtag aaaccgccca gctggtgcgc catcgcatga acataaccca gagaagcatt
     3841 attgaacgcc ataccggcga ggaactgggc ataagccatt gcttcacgcg ctttcgcatt
     3901 actgccatct tcaacggcta acggcaggtt ttcggcaatc atggtcacgg ctttcagtgc
     3961 acaagcgtca gtgatcggcg tggcggcaat agaaacatac gcttcgatag cgtgcgttaa
     4021 ggcatccata cccgttgcgg cggtcagtga cttcggcata ccaatcatca gagaggagtc
     4081 attgacagaa agcagcggag tgacatgttt atcaacaatc gccattttga tatgacgcgc
     4141 ttcgtcagtg atgatgcaga aacgggtcat ttcagaggcc gtacccgccg tggtattgat
     4201 ggcgatcatc ggcagctgcg gttttgcaga gcggtcaacg ccttcgtaat cgcgaatatc
     4261 gccgccattg gctgccacca gcgcaatacc ttttgcgcag tcgtgtggag aaccaccgcc
     4321 taaggagatc acgctatcgc aattattctc tttaagtaat ttcaaacctg cggcgacgtt
     4381 ttccgtggtg gggttaggtt gggtgccatc ataaataacg ctaaaaatat tgcgttcttc
     4441 cagtgctttt tgcacatcgc ccgccatacc taatttcgtt aacatactgt cagtgacaat
     4501 taaggtacgg gtaaatccat aatctgccat catattcatt gcatcagtca atgaatcagc
     4561 gccgatgaca ttcacagaag gaataaagaa agttgaagat gccataacac actcctgaaa
     4621 gtgtaaaaaa gggtgggcag cttactgagg attttcattc gaaaacatga tcattattat
     4681 taattatcaa aattaatc
//
LOCUS       DAZFKY010000264         4545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFKY010000264 DAZFKY010000000
VERSION     DAZFKY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(71..604)
                     /locus_tag="ACHKCL_004528"
                     /pseudo
     CDS             complement(71..604)
                     /locus_tag="ACHKCL_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(609..773)
                     /locus_tag="ACHKCL_004529"
     CDS             complement(609..773)
                     /locus_tag="ACHKCL_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765351.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(770..1450)
                     /locus_tag="ACHKCL_004530"
     CDS             complement(770..1450)
                     /locus_tag="ACHKCL_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5765352.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1538..1999)
                     /locus_tag="ACHKCL_004531"
     CDS             complement(1538..1999)
                     /locus_tag="ACHKCL_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765353.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1992..3032)
                     /locus_tag="ACHKCL_004532"
     CDS             complement(1992..3032)
                     /locus_tag="ACHKCL_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5765354.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3004..3555)
                     /locus_tag="ACHKCL_004533"
     CDS             complement(3004..3555)
                     /locus_tag="ACHKCL_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5765355.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3539..3766)
                     /locus_tag="ACHKCL_004534"
     CDS             complement(3539..3766)
                     /locus_tag="ACHKCL_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5765356.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3843..4250
                     /locus_tag="ACHKCL_004535"
     CDS             3843..4250
                     /locus_tag="ACHKCL_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765357.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT         1073 a         1243 c          978 g         1251 t
ORIGIN      
        1 ctcgtgcata gcttgttatt taaagtcaac aaaataagga aaattatacg catattgaag
       61 agtataaacc ttacatgttg attacatttt tgtaatcaac atcctgtttg gaatagccag
      121 cctttaatgg ataactattt ctgacaatgc aatgagtata atcaagtcca tcttccactg
      181 agaattagag gcggcatgct ttttccggct cttgccggat atccgtaatt gtccataatc
      241 tgcagattta taccttctgc atgacctgtc agcgaaaatt tgtccggtgt ttctacggga
      301 atgacatcaa aagttacacg cactcgcgtt accgtgtaga cctactttcc tgcacttgca
      361 agatcacagt ggtgtaaccg taacaggaat ttattctctg gaccggcagt aaatccctga
      421 gtggcgtggt tcccatatca atttcccgcc aggcagcctc cattgccagc gtacaggctg
      481 gagccatgac ctgcccttta aatctggccc gaccatccca ccggacgtgt tcttctcccc
      541 tgaacttagg tacagtcatc tccagtggca caaaagtgtc agcgccataa tttttgaccg
      601 ttatcgcgct acggatattt tgttgactgg tgaaaatcac cccgcagaat caggcttatt
      661 cccttaacct gggctttcat cctgaccgcc gcctcactac gaccaatcag actgccgatg
      721 cattttacct tcattgttag tatcatgatt tcaggcctgc accatccgct cattgcccgg
      781 acttccgaca aatcccggca accatatccc ggtgcttgtt cagctcccgc agcgcggcgc
      841 agactcgctc ccacttctgg acatgattct tcgccctacg cagttcgcga tttgccatat
      901 gcagcgatgg caaaatcaaa tcatcttctc gcgttgcagt aaacgatggc agcgactgca
      961 caatgtccgc cacagtttct gttttaatat cttcctgtgt tgcatctttc tgtaccggta
     1021 acgcaacacc ggctggctga ggaaaggctt taccatcggt ttccgctacc gattcaactt
     1081 tcggctctgc tggtaaatta tcacctggta tgcagtaacg aattttaccg ttctggttta
     1141 cgcgaatcag acgacctttg ctgattgcca ttgccagcgt tgaagcaact ttgcgggatg
     1201 ttgtaccgaa cagcgtagcc agttcatccg ccgtttgtgg tccgcgttgt tcaatcgtcg
     1261 cggttaaatc gcactctgag attttcgctt ctgttgccgt ggtggtttct tctggcgctg
     1321 gctgttcctg ctgaacgttg ttatcagcca cacgccaggt gtatacgctt ttatcaacga
     1381 agccagcctt tttcagttcc cacagctcgt tcagtacttc ttcacgactg atatcaagtc
     1441 gcgcagccag ctataccgac gtggcttttc ccatcgcttt cagtgcgtca aaaacagtct
     1501 ccataaattt cctcccggta aaaaatcact tctcaactca aacaaaacca gccgctttcc
     1561 ggcgttcata ttcctgtttc agcaactcaa ttggcgttgg ccccgacggg cgtttggggg
     1621 ccgccagttg tcgccggact ggcggaacgc tcaggccgtt actaacatgc tttgcccatt
     1681 tcgtcagttg ccgttctgca agccgtttta attccccttc ggtcatctgg cgctcaatcc
     1741 cctttgaacg catctcgagg caaatgtgat acagcacagg ctgagaccac gggtacttat
     1801 cacttccgtc gtatcgccag gactcattgc gccagcggcg gtactcctcc atcacagcat
     1861 ccaccgtcag accgaatgga ttggctccgc ttttcgaaat cagtgccaca aactcagcca
     1921 ggtccggagg ccatgtttca cccgcccggc agcggtccat gcactggcgg cagacctgcc
     1981 ggatttgctg ctcagtcatc gcgccaatct gtgcaatcca gagcttcgaa ggtgcggccc
     2041 cgttcttctg gatccagcgg ttcgaataaa ccacccccat gagttcccac agcttccagg
     2101 ccgtttccgt cgctgataaa tccgttttca cgttcccact gctcacgtgc tgcccgaatt
     2161 tcctgaactg cccgtgatgc ggtgccacct ggtgctgctg catggtttac ccctttgctg
     2221 actggtttaa cctgcgccct gacgtgattt acgtgacggg cgaatttctg ctcccactga
     2281 atctgcgtaa acactttccc ctccgctgcc cagtagtccc ggaaggcggc aagttcagca
     2341 ggtgtaaatt ctgtctccgg caaagccatc ccccacaacg cagcccgtcg tcgaaaatcc
     2401 cgtgacggat accagctatc gctcatcggg aattttccga tgggttcgct caggccatac
     2461 aggaatacag ggggggctgc ctgtaacgac aaaacttcct gctcactggt cggagcactc
     2521 tcgcgtgcgt tatgtgtggg gtttagatct ttgggttcct ttgggttccg tgatccgttt
     2581 ttgggtgtct ttgatggaaa atttgggtgt ctttggttat tttccatgca gctaagagtt
     2641 ctgtttttgg gtctgttttg tgctgaaaca taaccatttt cggtactgtt tttattaaca
     2701 gcaccaattt tacccacctt taaagactcc cgtttttggg tgtattcagg ctcggcaaca
     2761 ctttcttcta caccgataag tcggtacacc acaatttgct ttgttctgcc ttttctctca
     2821 ccggtatcaa caattaaccc aatctccatc aggtgtcgta agctgtcctg cacagtcttt
     2881 ttgttcagtt ccgttacttc tgccagtgca gatacagacg ggtatgcaca caaatcggca
     2941 ccgcacatat cagcaagcca ggtcaatact gacttactgg atgaactgcc ggttttcacc
     3001 tttttagccc atcgtagtgc atcgatactc atacgccccc ctggcagaca tttgtttatc
     3061 tgcaaagtaa tattggtatt gctgacgata cgcgtgcttg aaagcaatag ctttttctat
     3121 aagctcgtca gtctcacgtt ccacaacaac tggatccgca aaaagcagcc cggactccac
     3181 cacatcgcca tattctttgt ttaacccggc gatcatgtac gtaatgcttt ttccgtcagt
     3241 aatttcacaa tacaatctga aatcgctgat ccggatagcc tccataattg ccggaatcag
     3301 cgccgtgaat ttgtcacgct tatctctggt gtcgatagct ttccagcgtt cgaatatctt
     3361 cacccggtta acgcccagcg cccgttgatc aacctcgcca tcattaaacg tgacgcgttg
     3421 aacatcgatg ttcgggcgtt ctttcagagc ccagaatgct tccgtgatta atatcgtcgc
     3481 ctgctcctgt gtcattcctg gtcggcatac ccaggtatcc agagcctcac aaacctgttc
     3541 agtggtgatt ttcattgttc aaccgccccg cccgctttgc cttacgatat tcgtcataaa
     3601 ctttggggtc gtactgaagt tccccgccgg atgcctcttg caggcgcatc gcgcgacctt
     3661 caggaaccag ttccccccat gcagcaacgc ttgccagcct aactcctgcg acattggcaa
     3721 gctttgtttt gctgccaaaa aacgctatag catcaatttt caacatatcg aaacccttag
     3781 attttcctaa ggaaactaga tcgtagagaa acctaagtca agaaaaatta gaattaccta
     3841 atatgaaaaa cgaaaccttc ggtgctcgcc tcttatacag gcgaaaaaaa ttaaaactgt
     3901 ctcaggccgc attaggtaag ctggtcaaag tcgctcacgt aacaatttct caatgggaaa
     3961 gagatgaaac acagccagcg gggaagagat tattcgcact gagccaggcg cttcagtgct
     4021 cgccgacttg gcttcttttt ggagatgaag ataagcaacc aggcgaaccg atcccagata
     4081 atcagccagt caatctgaca gaagatcaaa aagagttgct tcaactgttc gacgcactgc
     4141 ctgagtcaga gcaaaaggct ctgttgtcag agatgcgtgc tcgagttgag aatttcaaca
     4201 aactttttga agaactactc aaagctcgca aaagaagcgc aaataaataa cccccctttt
     4261 tttcgcaact ctctgtaata aaaagcacaa actttcaaat acttgtgttt tttacatcaa
     4321 gaggcttagg tttttctaca caaaaagctt gaccataact cttaggctat tctaaattct
     4381 actcatcaag acaccgcacg gtgttctcag caaacagttc cgctacccgg cgttaagggg
     4441 aagcagagga tttctcagtg ggcgagtcaa acatcagaat ggaaggcgtc cagggatcag
     4501 caaagaaaca gcgatggcgc tttatattga tatcagcgcc attgc
//
LOCUS       DAZFKY010000265         4464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFKY010000265 DAZFKY010000000
VERSION     DAZFKY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1503)
                     /gene="rhsD"
                     /locus_tag="ACHKCL_004536"
     CDS             complement(<1..1503)
                     /gene="rhsD"
                     /locus_tag="ACHKCL_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO5765358.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPGAPLARYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGR"
     gene            complement(1570..3678)
                     /locus_tag="ACHKCL_004537"
     CDS             complement(1570..3678)
                     /locus_tag="ACHKCL_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5765359.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          935 a         1346 c         1128 g         1055 t
ORIGIN      
        1 cctgcccggt tcatcatatg cccggcggct ctccagcccg tccggggaca ccaccgccgt
       61 cagctggttc ccgtcgttat agtaaaattt cgtctcccgc ccgtccggtg tggtgatgtc
      121 cgtgatatcg ccggacacca cattcagacc gtactctgtc cgccgtcccg ccgcgtccgt
      181 ctgcgccgtg agccttcctg ccgcgtcata cccgctgtgc gtgacgctgc cgtccgccag
      241 ttcttttttc accacccgct tcagcccggc cccgccttct gtatgcagca cctcacgccg
      301 gttcaggctg tccgtgacgg tgatgcggtc ctgctcatac tggtagcggt aactcaggcc
      361 tgccgggttc agctgctcca ccacccgccc ggtatcgtcg tagcggtagc gcatctccgg
      421 ccttcccgcg taacggtgcg ccaccatccg gcccggatgc tgcgcgtcat acgtgaaagc
      481 gcgcacctgc gtattgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt
      541 gtaccgcgcc agtggcgcac cgggcaggct ttccgggtat gccgggtcgt gcgtcagcca
      601 caccgccgaa aggcggatac ctctgtcggg accgtattcg gtaccgggca gtgtgtcggg
      661 gaacgctgag tctgaaagag ggcggggggt gtcaggggaa gataaagaag cggtgtgctg
      721 tttacgggcc tcttccgccc gctgcgcctg cgtggtcagc accagacgga actcccgccc
      781 ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtca ccggcggcct cacgcctgta
      841 cgtcagcgtc cgcccgaagc ggtccgccat cccggtaagc acccggtacg gcggcagcgg
      901 cgctggcagt acgtcctcag cacccggcac ccgctctgac caccccagta tccaccacgg
      961 cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt aaccggatat ccggcggcag
     1021 cgccccccac agccgcgcca gcgtgtggcc gtccggctgc gctgccttac caccgcgcac
     1081 cagccacatt gactcgctgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
     1141 aatgctccgc ccgccgttgt cattgagtac cagtgcatca tcgcgtagct gtaagcggat
     1201 atcagaaggc gctttccagc cggggccaaa aatccccacc ggcgcaggcg tccgggtccg
     1261 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
     1321 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
     1381 gcccgggcac accgagcacg ccacgccagt tggcgcgcca attcttacac ctgccgaacc
     1441 ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
     1501 catgattcaa ttccttcttc tgtttatttt taatgcttgt ttgttactga ataccgaaca
     1561 ggtacgggat cagtactctc tcatatccgg cgtatcctgc ttttcatcag gggtcttcgg
     1621 tggggatttc gaggctgcac agttccagtt aatcaacagt gagtcaccat taacctgctc
     1681 cttaccctgc aaatgaattt ttgtgccgtt gagaaaaatt tgtccgtcct tcgtcagcac
     1741 cagccttgcc ttaccacagc agagctccag atgctcaccg gcggagtaaa tcgcggtctg
     1801 cccggtatca tcctttttcg ttttaccaac ggtgaaggta acgttatttc cgactttgac
     1861 gtcataaccc agcccgaccc tcaacgattt atgcaaaccg atctgcgagg actgcatcag
     1921 agccaccgaa gtgttcataa tgccacctac cgtcgtctgg tacgccacgc ccacggtcag
     1981 cgcacgaaca agtccaatcg tttccacctg aacagaaccc acgttaatga tctggttact
     2041 gcccaccgtc tctatctggt taacgcccac cgtctgtatc tgattgttgg taaccgcaat
     2101 catctgattg ttgccaatgg tctcagcatg gttgtttatc acatccgtgg tgcggttatt
     2161 cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt tccctgtcgc
     2221 atcgtcaaac ttcagttcat taaacccgct gcccttatag tttttcgaac gaatggtcat
     2281 ctgcgtcttc gtccccggca ggctgccggg tgtgcggttt tcctggtggt aggtacgccc
     2341 cataataatc ggctggtccg gatcgccgtt gaggaagtcc acaatcacct cctgacccac
     2401 acgcggtatc gccaggttgc caaagccggt gcctgcccac gcctgtgcca cacggatcca
     2461 gcatgaactg tcctggtttg acgggttata gcggtcccag ttaaatttca cccgcacgcg
     2521 accatgttcg tcgcagaaga tttcctcgcc tgccggtccc gtcacgacgg cgctctgcgg
     2581 gccatccacc agcggtttca gcaacggctg tggtcgccat gtccggtctg ccggtattac
     2641 cgcaaagtgg ttattcagcg tggtacctga acccctgcgt cccggcaccg cctgtggctg
     2701 ctcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     2761 ccccgtcagc acaattcgcc gtcccggcca tatttccgga gaacggcttg ttccgcgcgc
     2821 cacttctgcg ttgttgcgcc agccatccat ctgccagcgg gcaaagttct gcccgtgggc
     2881 acccttgaaa cgtccggggt agtcatacac ttcatactgt gtgcgctggt aatcctggta
     2941 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtccttggt
     3001 caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc atagggtgct
     3061 cacctcggta cgggtgttcg ggttccaggg gatctcaaag gactccggca gataacgcac
     3121 ggtgtcgcac aggaccaggc tctggtcgat acttttttgc gcgtgctcct cataaaagaa
     3181 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtttcgc cgtactggac
     3241 acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccactcgg tcacaccgtt
     3301 ttctttcagg atcgtagcga gtatgctttc gatgtcctca ttctggaaga tacggaagtt
     3361 ctggcgcagc cctgtgcgcc acagcggtgg gcacaccttc atgctgtaca gcatctggtt
     3421 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacgtc
     3481 atcgccctgc cataccgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
     3541 aagaaactgc tgactgacca gagaaaggtc gagggaaaaa agtgaagaga gtgactggtt
     3601 cagatgaaag gagaccaccg caaacgcgtc cggtggcagg ccgtccactt ccagcgtgaa
     3661 acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc acatattgca
     3721 tgtacaacat gtcgggcgga tgaaataagt gagtcgacat ctggcgttac cggggaatat
     3781 cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag agaaacgggt
     3841 gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac gttattcacg
     3901 acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca aaccaatatt
     3961 cataatgtct ttttattcac aacaataaat aatttaataa caatcattga aatgaatgat
     4021 gatttgtata aggaatatac gctaccgccc caccctgata acatgatctg cacattgcca
     4081 tcttaacgtc gttaaacact ggcttaatag tggatatttc aaattaggaa tttattaaat
     4141 tcacctgatg aatatcataa gcaaatagca tagaaaataa tgacaactcc cccgagggga
     4201 ccatttccaa agcactgcac caccacctgg tgcccggatg atcttgcttc agtggtaaga
     4261 gtacctgatt cattgttccc aaaatggtgt tttcccctgc ccctccaata atgtgcagct
     4321 ctcacgtgat aatctattga ttaggttata ttaagaatca atatttaaaa tacatccatt
     4381 ttcttgaata aatcaacatg tttattaatc aattgctgtt ttagctctga ctcatcaagc
     4441 tctccatatt tgtcctacag ataa
//
LOCUS       DAZFKY010000266         4246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFKY010000266 DAZFKY010000000
VERSION     DAZFKY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            9..593
                     /locus_tag="ACHKCL_004538"
     CDS             9..593
                     /locus_tag="ACHKCL_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5765360.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /locus_tag="ACHKCL_004539"
     CDS             673..1368
                     /locus_tag="ACHKCL_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5765361.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1397..3913
                     /locus_tag="ACHKCL_004540"
     CDS             1397..3913
                     /locus_tag="ACHKCL_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5765362.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3924..>4246
                     /locus_tag="ACHKCL_004541"
     CDS             3924..>4246
                     /locus_tag="ACHKCL_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5765363.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         1218 a          999 c          969 g         1060 t
ORIGIN      
        1 ggttataaat gaataaagtt acaaaaacag ctattgcggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgtaggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc
      301 tggaaacgcg ggataatggt accgtcccta acttccctaa cttggcaaaa gtgggctctt
      361 ttgctggcac agcagcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc
      421 ttatgccact aaatgctatg ggcaatgata acacagttta tcagatccct gcggactccg
      481 ctggaattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatccgaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctttca gataacgccc ttttccttcc tatttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcgatgatat ttaccttgtt gtgtagcttc
      721 tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac accttattta
      841 ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca
      901 ccgctaattc gtcttgaagc tggcgatgat tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cctgaaggga cgggtaagcg atgcgtggaa atcactggaa
     1141 tttaaacgta gtgatcattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggactag cagtcgataa agccgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tccagcatct ggcggaaaga acgtgaaatg ggctgcgatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 cacaggtcat cagggaatgc cacaacgaca ccaccaggga cataaacgca caccgaaaca
     1441 gttggcgctc attatcaaac gctgtttgcc gatggtgctc actggcagcg gcatgctttg
     1501 cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa ccacaaaaag
     1561 tggtatgcaa acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac taccaggaac
     1621 ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa ttacatttac
     1681 cgccaatgca gagcaacttc tgcagccaca gtttacggta gaacaactac gtgagctggg
     1741 tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg tgatcaactc
     1801 gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc agcgacttaa
     1861 tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct ccccttctcg
     1921 ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt ctgataaccg
     1981 ttaccgccag ggcaatcgta gccaacgaca gtacctaaat atgcaaaatg gtgccaattt
     2041 tggcccctgg cgattacgta actattctac gtggacacgc aacgatcagg cgtcaagctg
     2101 gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaagtctc agttgcttct
     2161 gggagaaagc gccaccagcg gcagtatttt ttccagctac aactttactg gcgtgcaact
     2221 cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa cggtacgcgg
     2281 tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga tctatcaaag
     2341 caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca acagcggcga
     2401 tttagaagtc acgattgaag aaagtgacgg tacgcaacgt cgctttatcc agccttattc
     2461 ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg ctggacgcta
     2521 tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca cggcaatata
     2581 tggtttgaat aatactttta cgctgtatgg cggcctgctc ggttctgaag attattatgc
     2641 gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga tggatatcaa
     2701 cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat ggcgtacgca
     2761 gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact atcgctatac
     2821 caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact ataacagtcg
     2881 ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg taagtctgta
     2941 tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga atatctctgt
     3001 tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat acagccgcta
     3061 cactgatcaa aataatgacc gcgcactctc tttgaatctc agtattccgt tagaacgctg
     3121 gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc caacccaaca
     3181 tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata gtctggaaca
     3241 aagtctggat gacgataaca accataacag tagcgtgaac gccagttacc gttcacctta
     3301 tggaaccttc agtgccggat acagttacgg taatgacagt agccaataca attacggcgt
     3361 taccggcggc gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc tgggcaacgc
     3421 ttttgcgctt attgatgcta acggggcatc tggcgtgagg atacaaaact atccggggat
     3481 tgctactgat ccctttggct atgcagtggt tccttatctc acaacttatc aggaaaaccg
     3541 tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa caacacagtt
     3601 tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata tcggttatcg
     3661 cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg ctcttgccag
     3721 caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat atctctctgg
     3781 gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag atcaacaatg
     3841 tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac aaggcacagc
     3901 gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga cttttgttga
     3961 tatccacatc ctgcgcttat gcctcctcag aagggtgtgg agctgacagc actagcggtg
     4021 cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat aacgtgacag
     4081 gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct
     4141 ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac
     4201 atcagacagg atattacaaa ctcaatgaca gcctggatat taaaac
//
LOCUS       DAZFKY010000267         4234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFKY010000267 DAZFKY010000000
VERSION     DAZFKY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            241..2391
                     /gene="btsT"
                     /locus_tag="ACHKCL_004542"
     CDS             241..2391
                     /gene="btsT"
                     /locus_tag="ACHKCL_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5765364.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2441..2644
                     /locus_tag="ACHKCL_004543"
     CDS             2441..2644
                     /locus_tag="ACHKCL_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5765365.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2655..3611
                     /gene="yjiA"
                     /locus_tag="ACHKCL_004544"
     CDS             2655..3611
                     /gene="yjiA"
                     /locus_tag="ACHKCL_004544"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5765366.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3680..>4234)
                     /locus_tag="ACHKCL_004545"
     CDS             complement(3680..>4234)
                     /locus_tag="ACHKCL_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO5765367.1"
                     /translation="KKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAA
                     LDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINR
                     FQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSS
                     EWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT          956 a         1106 c         1074 g         1098 t
ORIGIN      
        1 aatcgcttcg gttgtgatca tcatcacata aataaccttc ataaaccttg ctcaaaaccg
       61 ctcaaccgca aaactgaccg cttacgtccc taaaataacc actcagttat ttaccttact
      121 ttacgcgcgc gtaactctgg cagcatcatt gcaggatagc ggtcaattta cctcctcaaa
      181 cgcaacgcaa acctagaacg gcttcggcca actattaatc aatacatgcc aggttttact
      241 atggatacga aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca
      301 ttctgcctcg cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg
      361 gtcgcctctg tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag
      421 aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg
      481 aactacgttc cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt
      541 gctggtccgc tggtgggacc ggttctcgcc gcgcagatgg gctacctgcc tggcacgctg
      601 tggctgctgg cgggggtagt gctggccggt gcggttcagg actttatggt gctgtttatc
      661 tcctctcgcc gtaacggcgc atctctcggt gagatgatca aagaagagat ggggccagta
      721 ccggggacta tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct cgccgtcctg
      781 gcgctgattg tggttaaagc cctggcagaa agtccgtggg gtgtcttcac cgtttgctca
      841 accgtaccaa ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg
      901 ggtgaagtct ctgttattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg
      961 attgctcacg atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc
     1021 gcgctgattg gctatgcgtt tgtttccgcg ctgctgccgg tgtggctgat cctcgcacca
     1081 cgcgactatc tggcaacctt cctgaagatc ggcgttatcg tcggcctggc gctgggtatc
     1141 gtggtgctga acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc
     1201 ccgctgtgga aaggcgctct gttcccgttc ctgttcatca ccatcgcctg tggtgcggta
     1261 tctggcttcc acgcgctgat ctcttccggt acgacgccga aactgctggc taacgaaacc
     1321 gacgcgcgtt tcatcggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg
     1381 ctggttgctg catccatcat cgaaccgggt ctttacttcg cgatgaacac cccgcccgcg
     1441 ggccttggca tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc
     1501 atggcgcagc tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ttggggcttc
     1561 gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg
     1621 aaccgtgcag gtggtgcgcc gacgctggcg gtaggtatcg ctcatgtgtt ccacaaagtg
     1681 ctgccgatgg ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc
     1741 atcctgaccg cgctggatgc gggtacccgt tctggccgct ttatgctgca agacctgctg
     1801 ggtaacttca tcccgttcct gaaaaaaacc gactctctgg ttgctggtat catcggtact
     1861 gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc
     1921 ggcgttaaga gcctgtggcc gctgttcggt atctccaacc agatgctggc agccgtagcg
     1981 ctggtactgg gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact
     2041 gttgttccgg ctgtatggct gcttatctgc accacctggg cgctgggtct gaaactgttc
     2101 agcaccaacc cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt
     2161 gctaacggta ctgaccttac ggcgcagcag attgccaaca tgaaccacat cgttgtgaac
     2221 aactacacca acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc
     2281 tacggtttca aaacctggct tgcggtacgt aacagcgaca aacgtactga caaagaaaca
     2341 ccgtacgttc caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta
     2401 gccccgcttc ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca
     2461 ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa
     2521 ctacgtcgag catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt
     2581 cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg
     2641 ttaatggaga gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg
     2701 gcaaaaccac cctgctgcgc catattctta acgaacaaca cggctacaag attgccgtga
     2761 ttgaaaacga atttggcgaa gtctccgttg atgatcaact gattggcgac cgcgccacgc
     2821 agatcaaaac gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaagacg
     2881 cactactcga cctgctggat aatctcgata agggcaatat tcagttcgat cgtctggtca
     2941 ttgaatgcac cggcatggcc gatcccggcc cgattattca gacctttttc tcccatgaga
     3001 ttttatgcca gcgttacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg
     3061 atgagcagat gaaccagttc accatcgccc agtcccaggt tggctacgcc gaccgtattc
     3121 tgctgaccaa aaccgacgtc gcaggcgaag cagaaaaact gcgcgaacgc ctggcgcgca
     3181 tcaacgcccg tgcaccggtc tacaccgtta cccacggcga catcgacctg ggcctgctgt
     3241 tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccact
     3301 ttatcgcgga taaacaaaac gatatttcgt cgattgtggt agaactggat tacccggtag
     3361 atatcagcga agtttcccgc gtgatggaaa acctgctgct ggaatcggcg gataaactgc
     3421 tgcgttacaa agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg
     3481 tccagcgcct ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa ccgcatagca
     3541 caatggtttt tatcgggatt cagttgccgg aggaagagat tcgggcagcg tttgcggggt
     3601 tgaggaagta agttcgggaa agatcaactt tttgagagtt ccaccgcaac attattacga
     3661 gtggaactct ttagatatgt cagaccaacc aaagcttctt caattgcgtt ttcatttttt
     3721 catgtctcat tttcagttca gccaatcccc attctgaaga attacaaaca tcttttgtta
     3781 aatcaaaaga gcttttacta ttaagtttct cataaataga tttctttttg ataaaatcat
     3841 agtgttgtgc ttcagaattt ttggaaccac ttatgagtgt taaattacct attttatgca
     3901 gccagtagac atgctcttga ggttggaatc tattaatcca gtattcatta actaatgctt
     3961 gaggaagaat atgctcaatt gtgattctcc ccgtataggt tttaatcacg ctctcatctt
     4021 gttgctccat atctaagcga agcaaaattg cttttattaa gttcacttga ttcggacgag
     4081 gctcatataa atcttcatcc agagcagcta taaaacctga attatctgca tgttggttta
     4141 tctgatttat cacagaatca aaaggcatat cattatttat tgctaccaga gctgaataac
     4201 aaaccatttc tctttggctt ttaatttgtt tttt
//
LOCUS       DAZFKY010000268         4234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFKY010000268 DAZFKY010000000
VERSION     DAZFKY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(129..548)
                     /locus_tag="ACHKCL_004546"
     CDS             complement(129..548)
                     /locus_tag="ACHKCL_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765368.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            628..882
                     /locus_tag="ACHKCL_004547"
     CDS             628..882
                     /locus_tag="ACHKCL_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765369.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            879..1301
                     /locus_tag="ACHKCL_004548"
     CDS             879..1301
                     /locus_tag="ACHKCL_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5765370.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            1379..2167
                     /locus_tag="ACHKCL_004549"
     CDS             1379..2167
                     /locus_tag="ACHKCL_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765371.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQINTVSFPDSAIPPGFRG"
     gene            2174..2920
                     /locus_tag="ACHKCL_004550"
     CDS             2174..2920
                     /locus_tag="ACHKCL_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5765372.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELVTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEK"
     gene            2943..3704
                     /locus_tag="ACHKCL_004551"
     CDS             2943..3704
                     /locus_tag="ACHKCL_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5765373.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPAELPEETTMATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PTGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIFDSSSRIVSEK"
     gene            3720..4142
                     /locus_tag="ACHKCL_004552"
     CDS             3720..4142
                     /locus_tag="ACHKCL_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5765374.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT         1186 a          919 c         1121 g         1008 t
ORIGIN      
        1 ggtaggtata taatgtactt tgtgttcatc attgtaaagt actttaagta cattttatgt
       61 ataaaaaaat gagacgggat aaagtgaagc acaaacccgg agggggacgc taccggattt
      121 atgctggttt aagaggcttt ttgttttttc tttcgtgcta actcttcgta aattgcattg
      181 tacttctgtt ttttctcctc aagagttttt aaaagttcat ctgtctcact gtcagggagc
      241 tcgtccagaa ggtcaatgat gattttttgt cttggattta actcctgata gaaacgtatc
      301 tgtccacttt cttctgtatc ctctcccaaa agataggttg gtgttgttcc aatgagtgtt
      361 gctaattccc ttaatttctc tcggcgagga attgtttcac cattaaacca tttgctaacc
      421 gcttttggtg ttaatttcat tcgacgggca atttctgcct gccttccatg ttgttcataa
      481 ccagcgtttt cacaggctag cgcaagccta ctggcgaact ctttacgcgc tttatcttca
      541 tgaaccataa gttcaatgat attcgctctt gaatgtactg tcagttctgt tatagcatgt
      601 actcaaagtt cacattgtga gggtgatatg aaccagaaaa cacttgaaga tgtaatcaaa
      661 actgttcgcg tttctgttgt ggccgacgtt tgtggtgtca gccaaagagc aatctacaaa
      721 tggatggata acggtaaatt gcctcgcaca gaatataccg gcgaaacaaa ttacgctgaa
      781 aaaatcgctc atgcatcaaa cggattattt tctgccgatg caattttaac tattggcaga
      841 aataaaacta ctacgaaaaa gctgatggga gttgattcat gaaaatcaag catgagcaca
      901 tcgaatcagt gttgttagcc ctggcagccg aaaaagggca ggcgtgggtc gctaacgcaa
      961 ttactgaaga atatctgcgc caggggggcg gcgaattgcc cctggtacca ggcaaggact
     1021 ggaacaatca gcagaatatc tatcatcgtt ggttgaaagg tgaaacgaat gcgcaaaggg
     1081 aaaaaattca gaaactgatc cctgcggttc tggcaattct tcctcgcgag ctgcgtcacc
     1141 gactctgcat cttcgatacc ctggaacgcc gtgcattact ggcggcacag gatgcactga
     1201 gtacggcaat tgatgcgcat gatgatgcag tccaggccgt ttaccgtaaa gcacatttca
     1261 gcggcggcgg ttctcctagc gattctgtcg tagtgcattg attgaaatta atcgtaccga
     1321 actgttttgt tcggtatcag ttaaatgtaa cgctgcgagc gttacaaggt gaaaacaaat
     1381 ggcttcaaac tggataaagc tcgaagttat tacgccggat aagccggaaa tattcaggct
     1441 tgctgagatt ctgaatattg atccagatgc cgcattaggg aaagtcattc gcttctgggc
     1501 atgggcggat caacaaatga tagacggtaa cgcagagtgt aacgctcgcg gcgttacaaa
     1561 aagtgcaata gatcgcatca cttttatggc tggttttgct gatgcgttaa ttcaggttgg
     1621 atggctggtc gaaactaatg gtgtgttgtc gcttcctaac tttgagcgcc ataacgggaa
     1681 aagctctaaa aaacgggcgg ttacaaacga gagagtaaca aaaatacgag aactgaaacg
     1741 aaaaggtaac gctgccagcg ttacaaaaac ggatcaaaaa gcgttaccag aggaagagaa
     1801 agaggaagat ataaatactt atctccccct aaatccccct cgccaaaaac gagcgtctaa
     1861 aaaattcgaa ccggaggcta tcgagctgcc tgactggttg ccggaaacac tctggcatga
     1921 gtgggttcag ttcaggcagg cattgcgaaa accgattcga acggagcagg gcgctaacgg
     1981 ggcgatacgg gagctggaaa aattccgcca gcagggtttt tcacctgagc aggtgattcg
     2041 acacagcatc gccaatgaat accagggctt gttcgcgccg aaaggtgttc ggccagagac
     2101 gttgctccga cagattaaca ccgtctcgtt tccggacagt gcgatcccgc caggcttcag
     2161 ggggtaacag accatgaaaa atattgcgac aggcggcgtt ctggaacgta tccgcagact
     2221 ggccccgcca catgtaaccg cgccattcag gacggtggcg gagtggcgcg agtggcaact
     2281 tgcagaaggc cagaaacgtt gtgaggagat caaccgtcag aatcgtcagt tgcgggtgga
     2341 aaaaatcctg aatcgctccg gcatacagcc gttgcaccgc aaatgctcgt ttgcgaatta
     2401 ccaggtgcag aacgacggcc agcgatacgc gttgagtcag gcgaaatcta tcgctgacga
     2461 actggttacc ggatgcacaa atttcgcgtt cagcggaaaa cctggtaccg gaaaaaacca
     2521 tctggcggcg gctatcggga atcgcctgct gaaagatggc cagacagtga ttgtggttac
     2581 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gacgggcagt caggcgaaaa
     2641 atttttgcgg gaactgtgcg aagttgatct gctggttctt gatgaaattg gtattcagcg
     2701 cgagacgaaa aacgagcagg tggtactgca ccagattgtt gatcgccgga cagcgtcaat
     2761 gcgcagcgtg gggatgctga caaacctgaa ctatgaggcc atgaaaacat tgctcggcga
     2821 gcggattatg gatcgcatga ccatgaacgg cgggcgctgg gtgaatttta actgggagag
     2881 ctggcgcccg aatgttggtc agccaggtat tgagaagtaa tttttaccgg gaggaaattt
     2941 taatggagac cgtttttgac gcactgaaag cgatgggaaa agccacgtcg gtagaactgg
     3001 ctacgcgact tgatatcagt cgagaagaag tgctgaacga gctgtgggaa ctgaaaaagg
     3061 ctggcttcgt tgataaaagc gtatacacct ggcgcgtggc tgataacaac gttcagcagg
     3121 aacagccagc gccggcagag ctgccggaag aaaccaccat ggcaacagta gcgaaaatct
     3181 cagagtgcga tttaaccgcg acgattgaac aacgtggccc acaaacggcg gatgagttgg
     3241 ctacattgtt cggtaccaca tcacgcaaag tagcttcaac gctggcaatg gctatcagca
     3301 aaggacgcct gattcgcgta aaccagaacg gtaaatttcg ttactgcata ccgggcgata
     3361 atttaccagc agagccgaaa gcagcatcgg tagcggaaac tgatggtaag gcctttcctc
     3421 agcccacagg tgttgcgtta ccagtacagg aggctgcaac acaggaagat attaaaacag
     3481 aaacggtggc ggacattgtg cagtcgctgc catcgtttac tgaaacgcga gcggatgacc
     3541 tggttttacc atcgctgcat atggcaaacc gcgaactgcg tcgggcgaaa aatcatgtcc
     3601 agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg gatattgttc
     3661 gacagatttt cgattcctcc agtcgtattg tgtcggaaaa gtgattgccg gaggcaccta
     3721 tggcaaaagt atttacacca gaagagcggg aaaaaattaa agggcaggtt gttgaacttg
     3781 tacgtctgag cggtcgcgag acgttacggg ctctggaggc taaaaccggt gcatcaaggt
     3841 attacataag cactctcgcc agagaactgg tcgccagtgg tgatgtttac aattcaggct
     3901 acggattatt cccgtctgag caggcgcgta aagactggca aaacgcccgc aaaaaactat
     3961 caagggcaaa ggtgaagaaa ccggttgtgg ttgatccgga tcttatctgg tcattaccag
     4021 acggagaaat acgccgctac gacaggcgtc tgaacataat ctgtagcgag tgccggaaga
     4081 gcgaagctat gcagcgtgta ctggctttct atcagggtaa ttttcagaag gtgctgttgt
     4141 gagccaaatt aacaatcgga acttcgtgaa gagaaagcat aatccaaatc tgaataatta
     4201 agttcagcac tgtaaataaa atttaatcct taac
//
LOCUS       DAZFKY010000269         4225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFKY010000269 DAZFKY010000000
VERSION     DAZFKY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            225..1157
                     /gene="rfaD"
                     /locus_tag="ACHKCL_004553"
     CDS             225..1157
                     /gene="rfaD"
                     /locus_tag="ACHKCL_004553"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5765375.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1167..2213
                     /gene="rfaF"
                     /locus_tag="ACHKCL_004554"
     CDS             1167..2213
                     /gene="rfaF"
                     /locus_tag="ACHKCL_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5765376.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2217..3209
                     /gene="rfaC"
                     /locus_tag="ACHKCL_004555"
     CDS             2217..3209
                     /gene="rfaC"
                     /locus_tag="ACHKCL_004555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5765377.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3206..>4225
                     /gene="rfaL"
                     /locus_tag="ACHKCL_004556"
     CDS             3206..>4225
                     /gene="rfaL"
                     /locus_tag="ACHKCL_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5765378.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMML"
BASE COUNT         1103 a          969 c         1037 g         1116 t
ORIGIN      
        1 atgcttccgt tatttatcat acatcggaat attgatacta aagcactatt cacatgcaaa
       61 accaacatcc gccatgaagg actagctaaa acccaaacta gtttgttgca attagcatcc
      121 ttgcacctct atgtaaaggg ctgaagggat tcggatgtga tggtatgatt acagacattc
      181 gtgtctgaga ttgtctctga ctccataatt cgaaggttac agttatgatc atcgttaccg
      241 gcggcgcggg ctttatcggc agcaacatcg ttaaagccct gaatgataaa ggcatcaccg
      301 atattctggt ggtggacaac ctgaaagacg gcaccaagtt tgtgaacctg gtggatctga
      361 atatcgcaga ctatatggat aaggaagact tcctgatcca gattatggct ggcgaagagt
      421 tcggcgatgt cgaagcgatt ttccacgaag gcgcgtgctc ttccaccacc gagtgggacg
      481 gcaagtatat gatggataac aactatcaat actccaagga gctgctgcac tactgcctgg
      541 agcgtgaaat cccgttcctg tacgcttctt ccgcagccac ctacggcgga cgcacctccg
      601 actttattga atcccgcgag tacgaaaaac cgttgaacgt ctacggttac tcaaaattcc
      661 tgtttgatga atatgttcgc caaatcctgc cggaagcgaa ctcgcagatt gttggcttcc
      721 gctatttcaa cgtttatgga ccgcgtgaag gccataaagg cagcatggcg agcgtcgctt
      781 tccatctcaa cacccagctt aataacggtg aatcaccgaa gctgttcgaa ggtagcgaga
      841 actttaaacg cgacttcgtc tatgttggcg atgtggcaga tgtaaacctg tggttcctgg
      901 aaaatggcgt ttccggcatc ttcaacctcg gcaccggtcg tgcggaatcc ttccaggctg
      961 tagctgatgc tacgctggct tatcacaaga aaggccagat cgaatacatt ccgttcccgg
     1021 ataagctgaa aggccgctac caggcgttca ctcaggcaga tctgacaaat ctgcgcgcgg
     1081 cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt aacggaatac atggcctggc
     1141 tgaatcgcga cgcataagag ctctgcatga aaatactggt gatcggcccg tcttgggttg
     1201 gcgacatgat gatgtcgcaa agtctctatc gcacgctcca ggcgcgctat ccccaggcga
     1261 taatcgatgt gatggcaccg gcatggtgcc gtccattatt atcgcggatg ccggaagtta
     1321 acgaagctat tcctatgcct ctcggtcacg gagcgctgga aatcggcgaa cgccgcaaac
     1381 tgggtcatag tctgcgtgaa aagcgctacg accgcgccta cgtcttacct aactccttca
     1441 aatccgcatt agtgcctttc ttcgcgggta ttcctcatcg caccggctgg cgcggcgaga
     1501 tgcgctacgg tttactcaac gatgtacgcg tgctcgataa agaagcctgg ccgctaatgg
     1561 tggaacgcta tgtcgcgctg gcctatgaca aaggcattat gcgcacagca caagatctgc
     1621 cgcagccatt gttatggccg cagttgcagg tgagcgaagg tgaaaaatca tatacctgta
     1681 atcaattttc gctttcatca gaacgtccga tgattggttt ttgcccgggt gcggagtttg
     1741 gtccggcaaa acgctggcca cactaccact atgcggagct ggcaaagcag ctgattgatg
     1801 aaggttatca ggtggttctg tttggctctg cgaaagatca tgaagcaggc aatgagattc
     1861 ttgccgcttt gaataccgag cagcaggcat ggtgtcggaa cctggcgggg gaaacacagc
     1921 ttgatcaagc ggttatcctg attgcagcct gtaaagccat tgtcactaac gattctggcc
     1981 tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc cctgtatggt ccgagtagcc
     2041 cggacttcac accgccgcta tcccataaag cgcgcgtgat ccgcctgatt accggctatc
     2101 acaaagtgcg taaaggtgac gctgcggagg gttatcacca gagcttgatc gacattactc
     2161 cccagcgcgt actgaaagaa ctcaacgcgc tattgttaca agaggaagcc tgacggatgc
     2221 gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct ccatacgttg cccgcactca
     2281 ctgatgccca gcaggcaatc ccagaaatta agtttgactg ggtggtggaa gaagggttcg
     2341 cacagattcc ttcctggcac gctgccgttg agcgagttat tcctgtggca atacgtcgct
     2401 ggcgtaaagc ctggttctcg gcccctataa aagctgaacg caaagcgttt cgtgaagcgc
     2461 tacaagcaga gaactatgac gcagttatcg acgctcaggg gctggtaaaa agcgcggcgc
     2521 tggttacgcg tctggcacat ggcgtaaagc atggcatgga ctggcaaacc gctcgcgaac
     2581 ctttagccag cctgttttac aatcgtaagc atcatattgc aaaacagcag cacgccgtag
     2641 aacgcacccg cgaactgttt gccaaaagtt tgggctatag caaaccgcaa acccagggcg
     2701 attatgctat cgcacagcat tttctgacga acctgcctac agatgctggc gaatatgccg
     2761 tatttcttca tgcgacgacc cgtgatgata aacactggcc ggaagaacac tggcgagaat
     2821 tgattggttt actggctgat tcaggaatac ggattaaact tccgtggggc gcgccgcatg
     2881 aggaagaacg ggcgaaacga ctggcggaag gatttgctta tgttgaagta ttgccgaaga
     2941 tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa atttgtagtg tcggtggata
     3001 cggggttaag ccatttaacg gcggcactgg atagacccaa tatcacggtt tatggaccaa
     3061 ccgatccggg attaattggt gggtatgggg agaatcaagt agagtgtcgt tctacaagta
     3121 tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa tttaaatctt gaaataataa
     3181 ccaataagtt gacatcggag ataagatgac ctcaacatta tttttctctc tcgagaaaaa
     3241 aaactggata gcgtactgga acagagctct cgtattctta ttcattacca cctatttttt
     3301 gggtgggatt acaaggtata aacatcttat tgttattctt atgacaataa cgacaatcgt
     3361 ctatctctgc aaacggccaa aacactatct ctcactattt aaaacatttc tttttggtag
     3421 tgttgccata ttaactattg ctgcattgct gtcacttctt caatcccctg atgcaggtgc
     3481 tagcatgaag gaaattttca aagctattat tgagaatact ttactatgca caatagcaat
     3541 accgatcata ttgagagacg agaaaagaga agatgtcgaa aaaatcgttt tcttctcatt
     3601 tattagtgcg ttgggcttac gctgtttttc tgaattgatt acctattata aggactatca
     3661 acaagggata atgccattcg cagattatag acaccgtagc atttctgact cgatggtctt
     3721 tttattccct gcattgttaa atctctggct tatcaaatca gcaaaatacc gcatttcttt
     3781 tgtggttcta agcgttattt ttatttttct gatattagga actttatcca gaggggcctg
     3841 gctttccgtg ttagtcattg gattaatatg gattctgatg tttaaacaat ggaagttact
     3901 attagtagga gtaatggttg ccatcattgc attgtcggtt attttcacac ataaggagat
     3961 gaccgcaaag ctaacgtata aacttcaaca aactaatagt tcttatcgct atgcaaatgg
     4021 tactcaaggc agcgcactcg atctaatatt agaaaatcct gttattggtt atggttacgg
     4081 taacgttgca tataaagatg tctataataa acgtgtcatt gattatccag aatggacctt
     4141 tagacaatca atagggccac ataattttgc gctattcatc tggtttggca ctggtttatt
     4201 agggctggta agtcttatga tgcta
//
LOCUS       DAZFKY010000270         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFKY010000270 DAZFKY010000000
VERSION     DAZFKY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHKCL_004557"
     CDS             complement(<1..209)
                     /locus_tag="ACHKCL_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5765379.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKCL_004558"
     tRNA            complement(324..399)
                     /locus_tag="ACHKCL_004558"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKCL_004559"
     tRNA            complement(406..480)
                     /locus_tag="ACHKCL_004559"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKCL_004560"
     tRNA            complement(597..681)
                     /locus_tag="ACHKCL_004560"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKCL_004561"
     tRNA            complement(690..765)
                     /locus_tag="ACHKCL_004561"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKCL_004562"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKCL_004562"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5765380.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKCL_004563"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKCL_004563"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5765381.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKCL_004564"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKCL_004564"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5765382.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1047 c          945 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZFKY010000271         4151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFKY010000271 DAZFKY010000000
VERSION     DAZFKY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(129..797)
                     /gene="artM"
                     /locus_tag="ACHKCL_004565"
     CDS             complement(129..797)
                     /gene="artM"
                     /locus_tag="ACHKCL_004565"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5765383.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(797..1513)
                     /gene="artQ"
                     /locus_tag="ACHKCL_004566"
     CDS             complement(797..1513)
                     /gene="artQ"
                     /locus_tag="ACHKCL_004566"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5765384.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1520..2251)
                     /gene="artI"
                     /locus_tag="ACHKCL_004567"
     CDS             complement(1520..2251)
                     /gene="artI"
                     /locus_tag="ACHKCL_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5765385.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2269..2997)
                     /gene="artP"
                     /locus_tag="ACHKCL_004568"
     CDS             complement(2269..2997)
                     /gene="artP"
                     /locus_tag="ACHKCL_004568"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5765386.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3215..3730)
                     /locus_tag="ACHKCL_004569"
     CDS             complement(3215..3730)
                     /locus_tag="ACHKCL_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5765387.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1061 a         1068 c         1028 g          994 t
ORIGIN      
        1 tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct
       61 gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aaacccccgg cgaatgtacg
      121 cagccacatt aattccttcg ctcaaacgcc agcgctttgc gctcgatcag acgcatcatc
      181 agcgtcagca ggccgttaac gaccagataa ataatccctg ccgcgccgaa caccatgaca
      241 tcgtaggtgc gtccgtacaa caactggctg tatcccatca cttccatcag cgtaatggtg
      301 taagccagag aggtactttt gaacaccagc accacttcgt tggaataaga agagagcgag
      361 cgtttaaagg catacggcag caggatcgcc agcgtatctt ttttgctcat tcccagggcg
      421 ctacaggact gccactgacc ttccgggatc gcacgaattg caccgtaaaa cagctgcgtg
      481 gtatacgccg cactgttcag cgacaacgca atcagcgcac ataaccacgg ttctgacaac
      541 aaatgccaca gtgccggata ctcctgcaac gtcgggaact gacccggccc gtaataaatc
      601 aggaagatct gcaccagcag cggtgtaccg gtaaacagcg tgatataacc ccgcaccagc
      661 cacaccagca ccggcgtttt cagcgtcagg atgatggtaa aaatcaacgc cagaatcagt
      721 gccacaatca gcgaggcaac ggttagcgtc aggctggtgt gcagcccttt catcagttcg
      781 ggtaaatact caaacattag ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc
      841 gtttgagaat gtactgactg agcagggtga tcaccaggta aatcgccgcc gccacaatgt
      901 accaggtaaa tggttcctgg gtacgagtag cgatgctttt ggtttgcagc attaaatcat
      961 tcacactaat caaactgacc agcgcggtat ctttcagcag caccagccac tgattaccga
     1021 ggccaggcag cgcatgacgc cacatctgcg gcatcaccag acggaaaaag atagccgatt
     1081 tcgacagccc cagcgcctgg ccggattccc actgacccat cggcaccgct ttcaacgcgc
     1141 cgcgcagcgt ttgcgaggca taggcggcat acagcagtga cagagcgatg acaccacaaa
     1201 ggaacgggct aacgtcgaag ttttcaatct gcatctgcac cgggatctct acaatcccaa
     1261 gattgatagt gaagccatcc gaaagtgtca gcagcagctg cgaggagcca aaatagataa
     1321 acagcaccac cagaatttct ggcaggccac gcagaatggt taccagcgct gaacctgccc
     1381 acgcgacagg acgccatttc gcagactccc ataccgcaaa gaacatcgcc agcgccagcc
     1441 cgacaatcaa tgcacaaacg gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa
     1501 aaaattcatt catcaggaat tacttctgga accatttgtt gtagatggtt tcgtaagtgc
     1561 catctttctt cactttttcc agcgcagtgt tgagtttctg ctgcagctca gtgttgccct
     1621 gacgtaccgc gatgccgagg ccagtaccga agtaatcttt atcggtcact ttatcgccca
     1681 ctgccgctag tttcgggtta tctttcagcc attcagttac cactgcggtg tcaccaaata
     1741 cgctgtcgat acgaccgttt tgcagatcca gttttgcgtt ctggtagctg tcatacggca
     1801 cggtggtgat ttccgggtgc ttatccataa tgaatttctg gtgagtcgta ccattctgta
     1861 cgccgacttt tttgcctttc agctgatcaa cgctggtgta tttaccctgc tgacccacaa
     1921 acagggcaga gttgtcatag tacggcgtgg taaacagtac ctgcttttca cgttccgcgg
     1981 tgatatccat cccggccatc accgcttcca cgcgacggaa tttcaggctt gggatcaggc
     2041 tgtcaaatgc ctggttagag aaagtacagg tcgcatcaat ctctttacac agcgcctgtg
     2101 ccaggtcgac gtcaaaacca acgatctggt tgtttgcatc aatcgattca aacggaggat
     2161 aggaggcttc ggtagcaaaa cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg
     2221 caattaacgc ggcaatcaga acttttttca ttgttgttat cccgaatctt agtgagagag
     2281 atagttttta aatgcttcgg tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac
     2341 gatatgaccg ttttccatat acaccactcg gctggcggtt ttacgcgcca cttcaacttc
     2401 gtgggtgacg atcacctggg taatattcgt ttctgccagc tcacgaatga tgctgacgat
     2461 ttgtgccgta atttccgggt ccagtgcggc ggtcggttca tcaaacagca gtacctgcgg
     2521 ttccatcatt aacgcacggg caatagcaac acgctgctgc tgaccaccag aaagatgcag
     2581 cgggtaacga tcgctataag gtttgagacg cagacgttcc agcagttttt ctgcacgggc
     2641 cagcgcctga tctttactca accccagtac acggcagggc gcttcaatca ggttttgctg
     2701 cacggtcaga tgcggccaca ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa
     2761 atcgcgaatc gctttgtctg agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt
     2821 gagcgtaccg gagcgcggca tctcaagcag attgagtaca cgcagcagcg agcttttacc
     2881 cgcgccgctg gggccaagta acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc
     2941 gaacagcgcc tgatgcgcgc cgtagaagca attaatgccg tttaattgaa tactcattga
     3001 cactcgtata ctggcagtct gatagctatt gaggtcgaag atagtacctt tgacagaata
     3061 attatgcaat atttctgctt taaaagttaa aagcaaagcg cattattcaa taaacatagc
     3121 acaaaataac gggggcggtg gtcggcgagc ataaatgtcg gcattcctca cgaaatgccg
     3181 gacaatttac ggggtttatt ggttgatcaa ggcgttagcg attctcgatg gactgacgga
     3241 gcgtacccgc cgtggcatga acgctaccgc ctaagtagcg cacatcgtcg atcacccaac
     3301 actggccttc ctgaatcatt aacacttcat cctgccagcc ctggtcaccc tgcttgagat
     3361 ccacgcgcaa tggaatgtta cgggcatcac gattagggat agtcgatgca ctggcaacgt
     3421 gggcgctgtc tggcaaggtg gttcgactgg agaacggatc actgcttaac aattcacgat
     3481 gactattatc gcggctggca tcgctaagta gggttgccag tttgtcactc aaataggggc
     3541 gcagggcggt aatgtcattg ctgcgatgtt gaatgtggta gtcatagaat tgttgggcca
     3601 cgctgtcagg ccccccctgg acacaggaac cggtgcgggg accattatct ttataagctg
     3661 gcgtgacggt ggtacaggca ctcagcagca gtgcacatgg aataagtaga gatagtgaac
     3721 ggtagcgcat aatcatttcc ttattagctt gctgtctgcg tcacactacg accaaacgct
     3781 atcagattat ttaatacatt gtttttaata caattctgaa acaatctgaa cacctgattg
     3841 ggtttgagtc cattgctggc gacccactca aggttgctcg tagctgactt acttaaatta
     3901 cccaaacaat agacagtgta aaccatttgt gaggttgagc gtgttatatg gctgaaatga
     3961 tcatttaagg taaatatgat agttctgaaa tggtggtata aatcctcctg tagaggttaa
     4021 gagcatctgc aagaagccag tttcacattt ataacgcgtg ataaatgaac gcggttattg
     4081 tctaatcatt acaaattttt cacatgcgat aagagatcct gctattaaga tgattgccaa
     4141 aattacgttg t
//
LOCUS       DAZFKY010000272         4110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFKY010000272 DAZFKY010000000
VERSION     DAZFKY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            18..356
                     /locus_tag="ACHKCL_004570"
     CDS             18..356
                     /locus_tag="ACHKCL_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765388.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            596..850
                     /locus_tag="ACHKCL_004571"
     CDS             596..850
                     /locus_tag="ACHKCL_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5765389.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            1086..1160
                     /gene="tap"
                     /locus_tag="ACHKCL_004572"
     CDS             1086..1160
                     /gene="tap"
                     /locus_tag="ACHKCL_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5765390.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            1153..2010
                     /gene="repA"
                     /locus_tag="ACHKCL_004573"
     CDS             1153..2010
                     /gene="repA"
                     /locus_tag="ACHKCL_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5765391.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2923..3207
                     /locus_tag="ACHKCL_004574"
     CDS             2923..3207
                     /locus_tag="ACHKCL_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO5765392.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            3207..3482
                     /locus_tag="ACHKCL_004575"
     CDS             3207..3482
                     /locus_tag="ACHKCL_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5765393.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            3577..3783
                     /locus_tag="ACHKCL_004576"
     CDS             3577..3783
                     /locus_tag="ACHKCL_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765394.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(3883..4110)
                     /locus_tag="ACHKCL_004577"
                     /pseudo
     CDS             complement(3883..4110)
                     /locus_tag="ACHKCL_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1095 a         1029 c         1021 g          965 t
ORIGIN      
        1 ccaggagcag ataaacaatg aagctgatta tttttattct cattgttctg attattgcag
       61 cgttacttat ccggattatc ctcagaagtg taaatcagca ttctcctttg ctgatgcaat
      121 tgcatgcggc aggtatacgg accggtgatg cagagcgaat actgtccggc ggtgaatatg
      181 atgccagcca tttacgacct gaacgcagac gccgggatat tcttctggaa gaggttctga
      241 ggcaggctgg tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg
      301 gagaatggct gaatacaaca ggggctgctc agcagtcacc ggaacatcgt agctgacgcc
      361 ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc cccgctcccg
      421 gcgtttccat aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa
      481 tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa ttctcaagtc gctgatttca
      541 aaaaactgta gtatcctctg cgaaacgatc cctgtttaag tattgaggag gcgagatgtc
      601 gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa cgagcataca gaaaggggaa
      661 tccgctttct gatgcagaga aacaaagatt atcagtggcc cgtaaaagag cttcgttcaa
      721 ggaagtaaaa gtatttcttg aaccaaagta taaggccatg ctcatgcaaa tgtgtcatga
      781 agatggcctg actcaggctg aagttctgac cgcactgata aaaagtgaag cgcaaaaacg
      841 atgtgtgtga tgatgggctt acattcttga gtgttcagaa gattagtgct agattactga
      901 tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt
      961 ggatttacag gagccagaaa agcaaaaacc ccgataatct tcagaaagtt tggcgactga
     1021 gaagattacc ggggcccact taaaccgtat agccaacaat tcagctatgc ggggagtata
     1081 gttatatgct cggaaaagtt caagacttct ttctgtgctc gctccttctg cgcattgtaa
     1141 gtgcaggatg gtgtgactga tcttcaacaa acgtattacc gccaggtaaa gaacccgaat
     1201 ccggtgttca caccccgtga aggtgccgga acgctgaagt tctgcgaaaa actgatggaa
     1261 aaggcggtgg gcttcacctc ccgttttgat ttcgccattc atgtggcaca tgcccgttcg
     1321 aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc gggctattga tgcgctgctg
     1381 caggggctgt gttttcacta tgacccgctg gccaaccgtg tccagtgctc catcaccaca
     1441 ctggccattg agtgcggact ggcgacggag tctgctgccg gaaaactctc catcacccgt
     1501 gccacccggg ccctgacgtt cctgtcagag ctgggactga ttacctacca gacggaatat
     1561 gacccgctta tcgggtgcta cattccgacc gacatcacgt tcacaccggc tctgtttgct
     1621 gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc gcagccgtgt tgaatgggaa
     1681 aacaggctgc gcaaaaagca ggggctggat accctgggta tggatgaact gatagcgaaa
     1741 gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga cagagcttaa gtcacgggga
     1801 ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac gtcaggatat cgtcaccctg
     1861 gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct tcactgctaa tcgtgaggcg
     1921 gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga ttctgtcacg taaccgcaat
     1981 tacagccggc tggccacagc ttccccctga aagtgacctc ctcagaataa tccggcacgc
     2041 gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa aaaatcagca ccacatccca
     2101 aaaacaacct catcatctac cttctggtgc atccggtccc ccctgttttg atacaaaaca
     2161 cgtctcacag acgggcaatt ttgtttatcc acattaaact gcaagggatt tctctgtaag
     2221 attacaaccg ttcatgtcat aaagcaccag ccgccaggct tacagggtgc aatgtatctt
     2281 ttaaacaccc gtttatatct cctttaaact acttaattac attcatttaa aaaaaagact
     2341 tattcactgc ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag cggcgggccc
     2401 caaccggagc cactttagtt acaacacaca aaaacaacct ccagaaaaac cccggtcccg
     2461 cgcagaactg aaaccacaaa gcccctccct cataactgaa aaacggcccc gccccggccc
     2521 aaagggccgg aacagagtcg cttttaatta tgaatgttgt aactaagcat catcatcgct
     2581 gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa gcggccctga gggctcgcta
     2641 acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca
     2701 taaactctct catggctgaa agcgggtatg gtctggcagg gctggggggg gggtaaggtg
     2761 aaatccatca atcagtaccg gcttacgccg ggcttcggcg gttttacacc tgtgctacat
     2821 gtaacctccg tataccgcct tacatgcgct gacgcgcatt atttgccttg ttccgtctga
     2881 aagtaatcac tatgattaaa tatgattaac gactaatcgg atatgcaaat gaaaaacaat
     2941 accgcacaag caacaaaagt cattaccgcg catgtgccat tacctatggc tgataaagtc
     3001 gaccagatgg ccgccagact ggaacgctcc cggggttggg ttatcaaaca ggcgctttct
     3061 gcatggcttg cccaggagga ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg
     3121 acatccggac aggttattga ccatcaggct gtacaggcct gggcggacag cctcagtact
     3181 gacaatccgt taccggtgcc acgctgatgg aactgaagtg gagcagtaaa gcgctttctg
     3241 atttggcgcg gttatatgat tttctggtgc tagccagtaa acctgctgcc gccagaacgg
     3301 tacagtccct gacacaggca ccggtcattc tgttaactca tccacgtatg ggggaacagt
     3361 tatttcagtt tgaacccagg gaggtcagac ggatttttgc tggcgagtac gaaatccgtt
     3421 acgaacttaa tggccagact atttatgtat tgcgcctgtg gcacacacga gaaaacaggt
     3481 agattgtgtg tggggacaac ttaacatagc ctgcagatat taattctgga acattattat
     3541 caggccagat atttaaataa tcttgtagga gataaaatga attacgatga aataacgaaa
     3601 attaccgctg aacgtattag cgactacatg actgaagcag ttaatacaga ctctatagcc
     3661 gttgcggaaa tgtttcataa cgcagcatgg ggagtgcgaa cattgtggtt tgaactcgtc
     3721 acaaaaatag atatagatat acataaaaag aacagatatg cgagttatga tcttgatcga
     3781 taggaattta aatcccaaaa agattaaaaa aacacccaaa aacggatgtt tattcaacac
     3841 tgcctgcgtg ccatgagtgc accgccgtca attaaatgga tctatttcat cctgaccagc
     3901 atagtgctcc agacggctca tcggcaccgg cggttttttc aggtaagagc cgaagtacac
     3961 cgccacatgt gtggcgttat ccatcacccg ggatacgttg acattccagc cccgccggta
     4021 atgactgtcc aggaagcgat tcctgtcccg tttactgctt ccttctgctg ccagcgcatc
     4081 cggtatcacc aggtccggtg aggtgtactg
//
LOCUS       DAZFKY010000273         4107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFKY010000273 DAZFKY010000000
VERSION     DAZFKY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..517
                     /locus_tag="ACHKCL_004578"
     CDS             <1..517
                     /locus_tag="ACHKCL_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5765395.1"
                     /translation="ADNHTLSAGYAQSKVQDFKNIKGVNLQYRYEWDSPVSVVGSFSY
                     MKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHTKAKGDYE
                     WRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKADIYGKHSV
                     NGFTVGVGYRF"
     gene            653..1444
                     /locus_tag="ACHKCL_004579"
                     /pseudo
     CDS             653..1444
                     /locus_tag="ACHKCL_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1449..2819)
                     /locus_tag="ACHKCL_004580"
                     /pseudo
     CDS             complement(1449..2819)
                     /locus_tag="ACHKCL_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2839..3186)
                     /gene="tnpB"
                     /locus_tag="ACHKCL_004581"
     CDS             complement(2839..3186)
                     /gene="tnpB"
                     /locus_tag="ACHKCL_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5765396.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3183..3857)
                     /gene="tnpA"
                     /locus_tag="ACHKCL_004582"
     CDS             complement(3183..3857)
                     /gene="tnpA"
                     /locus_tag="ACHKCL_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5765397.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          922 a          987 c         1112 g         1086 t
ORIGIN      
        1 ggctgataat catacgctat cggctggtta tgctcagagt aaggtacagg actttaagaa
       61 tattaaagga gtgaatctgc aatatcgata tgagtgggat tctccggtca gtgtcgtcgg
      121 gagtttttcg tatatgaaag gtgattgggc ggacagtcat cgcgatgaag ctgacgattt
      181 ttacagacat caggctgata ttaaatatta ttcatttctc gcaggcccag cttatcgtct
      241 gaatgactat atcagttttt atggtttggt ggggatatct catactaaag cgaagggaga
      301 ttatgagtgg agaaacagtg tgggtgctga tgagtctgat ggatatttaa gtgagtctgt
      361 atcaaaaaaa tccactgatt ttgcttatgc agctggtgtt attattaatc cgtggggcaa
      421 tatgtctgta aatgttgggt atgagggaac taaagcagat atatatggaa aacattcggt
      481 caatggattt actgttggag ttggttatcg tttttaatca ttagacggga ctggataatt
      541 gcacttgcgt agtgaagttt actaaatgtt tgtattttaa tgaagtattc gacatattcc
      601 cttcagacgg aatatgtcga cggttatggt tttactggag catgagtatg ttatggcgaa
      661 actgataaat atatcagcgt ccactgagga gagaatgacg ccaggggaga ggagggtggc
      721 ttcacgactg gagagctttc tgaatgatga ttgctttgtc tggtacgata tcccggtggg
      781 acggaaaaac cgacatccgg attttgtcat tattgacccg gataacgggc tggtatttct
      841 ggaggtcaag gactggactg tatccacatt acgtaaagca aatcaggagc aggttacact
      901 ggaaaccgac ggtcttctga aatcagaaat aaatccgctg gtacaggttc gtcgttatgc
      961 ctgtgatacg gttaatgcct tacctgctga cccgtgctta cggcagaatg acggtcagta
     1021 taaggggcgg ctgaacctgg cctgggcgta tggtgtggtg tttacccgca ttacccgtca
     1081 gcaactgaag gcactgacag ggaataatga gaatgcggtg gaaaagattt ttccttcagc
     1141 acagacaatc tgtcaggatg aaatgacgca gtctgtttta cctgaggtat tccggcaaaa
     1201 aattgccggg atgtttacca cggggttcag aacccgggta acaccccgga tgagggatat
     1261 tctgcgcgcc catctgtttc ctgaagtgac cgtgaaacag aactcacaaa tcaaaattat
     1321 ggatattcag caggagattc tggccagaaa tattggtgac gggcatcgtg tcattcatgg
     1381 tgtggccggt tccggaaaaa ccatgattct tttgttccgt tgcctttacc tggcagaaac
     1441 cacgtccggt agttacgccg ccccagtgca actacccgga gggcgttttc acacaggtta
     1501 ttgtcgatct ccacccagcc attgcggcag tattcgttca gagcgtccca ttgcttcagc
     1561 agataggtga acgctttcgc tgtatccgag tggcgcgaca gcgtgttcat ctgcccctgg
     1621 agccactcat acaacgactg cattagcggt accgttctgg cttttctgac cgccagtcgc
     1681 tcttctgccg gactgccgcg gatctcagcc tcgatagcgt acagttcccc gatacgacgg
     1741 agcgcttctc ccgttactgt cgttggatgg cggacatgta catcgtggat cttgcgtcgg
     1801 gcatgtgcca tacaagccgc ttcggttgcc tgaccgcttt cgtaaagagc attgtaaccc
     1861 gcatatgcat cggcctgcag gatacctctg tagtccgcca gatgttgctg tgggtggatg
     1921 cctttgcggt cgggagagta tgagaaccac accgctgccg gcatggtcga accggcgttg
     1981 cgatcgtcac gaacatagac ccacagccgt ccggtacggg ttttaccctg acccggctcc
     2041 agtacattta ccggcgtgtc atcggcatgc actttacccg gcatcagcac atagtgcttc
     2101 agttcatcat acagcgggcg aagctgctct cccatgatgt caacccagcg ccccatcgta
     2161 ttgcggtgca gctccacgcc ctggcgggca tagatttccg actgacggta cagcggcaga
     2221 tgctcggcga acttagccat gattatgcgg gccagcagag ccggactggc gtaactgcgc
     2281 tcgatgggtt ttggtggctg cggagcctga actatacagt cgcaccggct gcaggcccgt
     2341 tttgggcgaa ccgtttcgat taccctgaac gcggtgttga tgatatccag ttgttcagag
     2401 atgctttctc ccagcggttt cagtttaccg ctgcagacgg ggcattcggt ttctgccggg
     2461 gagataacct gcctgtcacg gggaagtgtt gccggaagtg ctttgcggac gggagagtct
     2521 gatgttttcg gcgctgtctc tccggccatt gaggtgagtt gcaactgcgc ctcaccaagc
     2581 ctgttctgga gctcggttat acgcgtttct gcccgtgcga tcttcttttc tatcttctcg
     2641 cggcttttct cgctgctgcg accgaacaac attctctgca gtttagcgac cagcgctctg
     2701 agtgagctga tctcgcgggc atagccggtt atttcaccag acagacggac gataacagcc
     2761 tgctgtgcga tcagcagggc cttcagttgc tcgatgttgt cggggagtgt gttgttcatt
     2821 cccctgtttt atcacgggtt atatccggat gccaggccgt tctgtccgtt tgggatgttg
     2881 ccatgcgatc ccctccagta gcatggataa ctgagctggc gtcaggtgca ctttcccttc
     2941 ccgggttacc ggccagacga agcggccccg ttccaggcgt ttggcgaaca ggcataaccc
     3001 gtcacgatcg gcccacagta ttttcaccat tttgccactg cggccccgga agacgaagat
     3061 atgcccggag aacggggcat ctttcagcgt gttctgcacc ttcgaagcca ggccgttgaa
     3121 gccacaacgc atatctgtga tgccagcgat gatccagatt ctggtaccgg ttggcagcgt
     3181 tatcatcggg tacctccttt catttcgcgg attagcgccc gtaacatttc cggagtgaga
     3241 gggtcaaaca gttttaccac acctgattta agatgcagct cgcaccgtgg gacgtttccg
     3301 ggatcactct cagggcactc atcaggcttg ttacgccaga agggatttgt aactggtctg
     3361 gtcggctctg gcgtatcagt cagagccacc gggacaggca tgcattcctg tatgtcatca
     3421 tcgctcagta agccgtcctc gtactggctt ttccatttta acagcaggtt atcattgata
     3481 ccgtgctctc tggcgatccg ggcaacaaca gcaccgggct gtaatgcctg ttttaccagc
     3541 ctgattttaa attcacgact gtaggaggtt cgtcgttctt ttcgccatgt gccttcgctg
     3601 atttgaggct ctgttaattc cttctttctg ttggcataaa ggatggcgtc aagctgagct
     3661 aatgaaactg aatcgggcaa tggccatgcg ataccggatg caataaatcg ctgaaaaagc
     3721 gtatgtattg tggaatgact gagacctaga cgctgagcga tggcccggat ggtcagttta
     3781 tcttcaaatc ttaaacgcag agcatcaggc aaataagaac ggaagcaggg aatatctttt
     3841 tttgtctggg aattcatcgt tcgtgtccat ctatatagat gggcgcgatt gttgccagac
     3901 aggacaattt tcacaagacg tcgcagatgg ggcgcttaca caacaatggc gcgagggaaa
     3961 gctgctatta ccttcttcag agagccccca gctacttcct gtgactctcg atgcagctgc
     4021 cgaacagcca gaatcgctcg cagaggaccc ggaaaccctc agtatcagct gttgtcaacg
     4081 acggatgact attgcaccgt tcgtgac
//
LOCUS       DAZFKY010000274         4102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFKY010000274 DAZFKY010000000
VERSION     DAZFKY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..929
                     /locus_tag="ACHKCL_004583"
     CDS             <1..929
                     /locus_tag="ACHKCL_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO5765398.1"
                     /translation="CLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVL
                     NLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEG
                     SYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLII
                     DNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGL
                     RQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSL
                     NEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1138..2103
                     /gene="lipA"
                     /locus_tag="ACHKCL_004584"
     CDS             1138..2103
                     /gene="lipA"
                     /locus_tag="ACHKCL_004584"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5765399.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2204..2407)
                     /gene="tatE"
                     /locus_tag="ACHKCL_004585"
     CDS             complement(2204..2407)
                     /gene="tatE"
                     /locus_tag="ACHKCL_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5765400.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2536..3324)
                     /locus_tag="ACHKCL_004586"
     CDS             complement(2536..3324)
                     /locus_tag="ACHKCL_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5765401.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3417..3800
                     /gene="crcB"
                     /locus_tag="ACHKCL_004587"
     CDS             3417..3800
                     /gene="crcB"
                     /locus_tag="ACHKCL_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5765402.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3854..4063)
                     /gene="cspE"
                     /locus_tag="ACHKCL_004588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3854..4063)
                     /gene="cspE"
                     /locus_tag="ACHKCL_004588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5765403.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1069 a         1060 c          939 g         1034 t
ORIGIN      
        1 cctgtttaag cagaaaatcg tcagaaggta aaccacaaat atttacaacc ttacgaaata
       61 ttgatcttaa tcttctgact atttttgaag ctgtatatgt acataaaggg atcgttaatg
      121 cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag aaactgcgcg
      181 ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct accgcatttg
      241 cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc gcgctggata
      301 tcgaaggaag ctatgataag caacgaacga taactattgc gaccaccccc tcggtcggag
      361 ctctggtcct tcctgtcatt tatcgggcga ttaaaactca ctatccgcag cttttactgc
      421 gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc gatctcatca
      481 tagataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc accgacaata
      541 tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac agagagacta
      601 tcgacaacgc tgcacatgta ctcctgttac cggaaggaca aaatttcagc ggtctgcggc
      661 agagaattca agagatgttt ccggaccggc agatcagctt caccagctac aacattttga
      721 caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc cgtttttata
      781 acctgtttag ccgttgctgg ccgctggaaa aactgccttt tccgtcctta aatgaggagc
      841 aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc ctgcacggag
      901 taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga tcacagcgca
      961 gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc tttggctgct
     1021 ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt gataattaca
     1081 acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac gcacgctatg
     1141 agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa gatggccctt
     1201 atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg
     1261 aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc aatgcgcaaa
     1321 aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga atgcttcaac
     1381 cacggcacag caacgtttat gatcctgggc gctatttgta cccgccgttg tccgttctgt
     1441 gacgttgccc acggtcgccc ggtagctcct gatgccaatg agccagtgaa actggcgcag
     1501 accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg tgatgacctg
     1561 cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga aaaaagcccg
     1621 caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gtatggatcg tgctctggat
     1681 attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt accgcgtatt
     1741 taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct ggaacgcttt
     1801 aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggtct gggtgaaacc
     1861 aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg
     1921 ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta cgttagcccg
     1981 gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca tgctgcatgc
     2041 ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat ggaagttaag
     2101 taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg aagcgggttt
     2161 tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga
     2221 gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca
     2281 tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct
     2341 tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta atactaatct
     2401 cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccaaaga
     2461 atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg
     2521 ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt ttaagacggg
     2581 cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa tgagtgcagg
     2641 catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc tttgcccgat
     2701 atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca gcgcacgagc
     2761 ggcaagcaac gttgaccagt ggtgctcttt gagcggcccg cgaacccagg cggcaggaag
     2821 taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa agcgtaagtc
     2881 ataacaggtc atcagaccga ccttcatccc ctccacctcg agtaacggag caatttcatt
     2941 accagcatca acacggcgtg attcctgaat ggcaaatgca tcatagagat gcaatttggc
     3001 ataacgggcg acgatgtttc ctgcctgtag cgccaccagc atattccatg cgcgccccgg
     3061 cgttgaagga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac tttctcgccg
     3121 taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata gatctgcatc
     3181 atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt cgttttccgc
     3241 cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag atgtaacagc
     3301 aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa ttcgccaacg
     3361 acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat tttgcagtgt
     3421 tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg agatggctgt
     3481 taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg gcagcaaacc
     3541 tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg acgaacattg
     3601 atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca accttctcaa
     3661 cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg gcattactga
     3721 acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc tggctgtttt
     3781 cggcctcaac cgcacactaa agaccaaaaa aaacccgctg attaagcggg ttttgaattc
     3841 ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag
     3901 tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg
     3961 cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt
     4021 tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat
     4081 gaaaaataga cacaaatgct gt
//
LOCUS       DAZFKY010000275         4091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFKY010000275 DAZFKY010000000
VERSION     DAZFKY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            188..796
                     /locus_tag="ACHKCL_004589"
     CDS             188..796
                     /locus_tag="ACHKCL_004589"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5765404.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            894..2285
                     /gene="selA"
                     /locus_tag="ACHKCL_004590"
     CDS             894..2285
                     /gene="selA"
                     /locus_tag="ACHKCL_004590"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5765405.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2282..>4091
                     /gene="selB"
                     /locus_tag="ACHKCL_004591"
     CDS             2282..>4091
                     /gene="selB"
                     /locus_tag="ACHKCL_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5765406.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          933 a         1051 c         1193 g          914 t
ORIGIN      
        1 gcaaaaccct acagatttat aagaaagcct taagtgagat atccatcaga tagaaacaat
       61 taggaaaata tttatatcac catgataaat atgtgattta tttggaagta acaaaccatc
      121 aacattttct gccctttttc ccatcaccag gccatactga tatttttcct cgcacaaagg
      181 actcggcatg aaactcgtcg gtagctacac cagcccgttt gtacgcaaac tttctattct
      241 gttgttagaa aagggcataa ctttcgaatt tattaatgaa ctgccctata acgcggacaa
      301 cggcgtgacg caatttaacc cgttaggaaa agtgccggtg ctggtgaccg aagagggcga
      361 atgctggttt gattcgccga tcatcgctga atatattgaa ttaatgaatg tcgctccggc
      421 gatgttgccg cgcgatccgc tggagtcgtt gcgggtgcgc aaaattgagg cactggcgga
      481 tggcattatg gatgccgggc tggtatcggt gcgtgaacag gcgcgtccag cggcgcagca
      541 gtctgaagat gaattgttac gccagcggga gaaaatcaac cgcagtctgg atgtgctgga
      601 aggatatctg gtcgatggca cactcaaaac cgatacggtc aatctggcga ctatcgccat
      661 tgcctgtgcc gtcggatatc tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg
      721 cccacactta gtcaaactgg tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga
      781 accgccaaag gcttgatgcg cgatatgtcc tcctgaccca tctcacgtta caatccgtgg
      841 ttatgttaaa cgcccttctc cgtgtgagag ggccttgatc agccaggttt cctatgacaa
      901 ccgaaacgcg ttccctctat agtcaacttc cggctattga tcgcttattg cgcgatagct
      961 ccttcctttc tttgcgtgat acttatggtc acacccgcgt ggtggaattg ttgcgtcaga
     1021 tgctcgacga agcgcgagaa gtgattcgtg gcagccagac gctgcctgcg tggtgtgaaa
     1081 actgggcgca agaagtcgat gcccggttga cgaaagaagc gcagagcgcg ctgcgtccgg
     1141 tgatcaacct gacgggaacc gtgctgcata ccaaccttgg gcgagcttta caggcggaag
     1201 ccgcggtgga agccgttgcg caggctatgc gttcgccagt gaccctcgag tacgatctgg
     1261 acgacgccgg acgcggacat cgcgatcggg cgctggcgca gctgctgtgc cgtattacgg
     1321 gggcggaaga tgcctgtatc gtcaataaca atgcggcggc ggtgttattg atgttggcgg
     1381 ccactgccag cggaaaagag gtggtggtat ctcgcggcga actggtggag attggcggcg
     1441 cgtttcgtat tcccgatgtt atgcgtcagg caggctgcac cctacacgaa gtagggacca
     1501 ccaaccgcac gcacgcgaat gattatcgtc aggcggtgaa tgaaaatacc gcactgttga
     1561 tgaaagtaca taccagtaac tacagcattc aggggttcac caaagcgata gatgaagcgg
     1621 aactggtggc gctcggcaaa gagctggatg ttcccgtagt gactgattta ggcagtggct
     1681 cgctggtcga tcttagccag tacggtttgc cgaaagagcc aatgccgcag gagttgattg
     1741 cggcgggcgt cagtctggtg agcttctccg gcgacaagtt gttaggcggg ccgcaggcag
     1801 ggattatcgt tggtaaaaaa gagatgatcg cccgcctgca aagccacccg ctgaagcgtg
     1861 cattacgcgc ggataaaatg accctcgcgg cgctggaagc cacgttgcgt ctttatttac
     1921 accctgaagc tctgagtgaa aaattaccga ccctgcgcct gcttacccgc agcgcagagg
     1981 tcattcaaat ccaggcacaa cgtttacagg ctccccttgc cgcacattac ggcgcggagt
     2041 ttgcggtaca ggttatgcca tgtctttcgc agattggcag tggttcgctg ccggttgatc
     2101 gcctgccgag cgcggcatta acgtttacac cccatgatgg acgcggtagc caccttgagt
     2161 cattagccgc ccgctggcgt gaattgccag tgccggtgat tggtcgtatt tatgacggac
     2221 gattgtggct ggatttacgc tgccttgaag atgagcaacg gtttttggag atgttgttga
     2281 aatgattatt gcgactgccg gacacgttga ccacggcaaa acaaccttat tgcaggcgat
     2341 tactggcgta aatgctgacc gtctgccgga agaaaaaaag cgcggcatga ccatcgatct
     2401 cggctatgcc tactggccgc agccggatgg tcgcgtgcct ggttttatcg acgtacccgg
     2461 tcatgaaaag tttctttcca acatgctggc gggcgttggt ggtatcgatc acgcgctgtt
     2521 ggtggtggcg tgcgatgacg gcgtgatggc acagacccgt gagcatctgg cgattttgca
     2581 gctgaccggt aacccgatgc tgacagtggc gctgaccaaa gccgatcgcg tagacgaagc
     2641 gcgtgttgat gaggttgaac gccaggtaaa ggaggttctg cgggaatacg gttttgctga
     2701 ggcaaaactg tttatcaccg cagcaaccga aggtcgggga attgatgccc tgcgcgagca
     2761 tctgcttcag ttgccggaac gcgagcacgc cagccaacat agtttccgcc tcgcgattga
     2821 ccgcgcattt accgtaaaag gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga
     2881 agtgaaggta ggcgattcac tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc
     2941 gctgcatgcg caaaaccagc caacagaaac cgccaatgcc gggcagcgta tcgcgcttaa
     3001 catcgcgggt gatgcggaaa aagagcagat taaccgtggc gactggctgc ttgccgatgt
     3061 gccgccagag ccgttcacac gggtgattgt cgagcttcaa acccatacac cgctgaccca
     3121 gtggcagccg ctgcatattc accacgccgc cagccacgtc acgggacgcg tttcactgct
     3181 ggaagataac cttgctgaac tggtcttcga ccccccgtta tggctggcag ataacgaccg
     3241 cctggtattg cgcgatatct ctgcccgcaa cacgctggcc ggagcgcgcg tcgtgatgct
     3301 taacccgccg cgtcgcggta aacgtaaacc agagtatctg caatgcctgg cttccctcgc
     3361 cagggcacag aatgatagcg aagcgttagc cgttcacctg caacgcggcg cggttaatct
     3421 tcctgatttc gcctgggcgc gccagctcaa cggcgaaggg atgcgcgaac tgctgcaaca
     3481 gcctggctat attcaggctg gttatagctt gttgaatgcg ccggttgccg ctcgctggca
     3541 acggaaaatt ctcgacacat tagcgactta tcatgagcaa catcgcgatg aacctgggcc
     3601 tgggcgcgaa cgtctgcgac gtatggcgtt gccaatggaa gatgaagcgc tagtactgtt
     3661 gctgattgaa aagatgcgcg aaagcggcga catcctcagt catcacggct ggctgcatct
     3721 gccggatcac aaagcgggct tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc
     3781 gctgtttggt gacgagccgt ggtgggtgcg tgatctggca aaagagacgg gtaccgacga
     3841 gcaggcaatg cgcctgacgc tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt
     3901 caaagatcgt tattaccgta acgatcggat tgtcgaattt gccaatatga tccgcgatct
     3961 cgatcaggag tgtggttcaa cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg
     4021 aaagctggca attcagattc tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg
     4081 aaatgatcat t
//
LOCUS       DAZFKY010000276         4044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFKY010000276 DAZFKY010000000
VERSION     DAZFKY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(293..397)
                     /locus_tag="ACHKCL_004592"
     CDS             complement(293..397)
                     /locus_tag="ACHKCL_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765407.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(513..1100)
                     /locus_tag="ACHKCL_004593"
     CDS             complement(513..1100)
                     /locus_tag="ACHKCL_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5765408.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDG
                     EFFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1103..1294)
                     /locus_tag="ACHKCL_004594"
     CDS             complement(1103..1294)
                     /locus_tag="ACHKCL_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765409.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            complement(1296..1733)
                     /locus_tag="ACHKCL_004595"
     CDS             complement(1296..1733)
                     /locus_tag="ACHKCL_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5765410.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(1720..2037)
                     /locus_tag="ACHKCL_004596"
     CDS             complement(1720..2037)
                     /locus_tag="ACHKCL_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765411.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            complement(1991..2308)
                     /locus_tag="ACHKCL_004597"
     CDS             complement(1991..2308)
                     /locus_tag="ACHKCL_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5765412.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            complement(2298..2600)
                     /locus_tag="ACHKCL_004598"
     CDS             complement(2298..2600)
                     /locus_tag="ACHKCL_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5765413.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            complement(2597..2914)
                     /locus_tag="ACHKCL_004599"
     CDS             complement(2597..2914)
                     /locus_tag="ACHKCL_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5765414.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(2911..3627)
                     /locus_tag="ACHKCL_004600"
     CDS             complement(2911..3627)
                     /locus_tag="ACHKCL_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5765415.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            complement(3661..>4044)
                     /locus_tag="ACHKCL_004601"
     CDS             complement(3661..>4044)
                     /locus_tag="ACHKCL_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765416.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
BASE COUNT          918 a         1194 c          830 g         1102 t
ORIGIN      
        1 atacagatga cgatcagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc
       61 ttgcctttcg gcgcgtaaga ggctaaccta catgtgtcta gcatgaaatt ggcctcagat
      121 taatgttagg cgtcttgcag gacgcgtaat gttaactggg gcttttctct gtctacctta
      181 cggtggcatg cccgaggcag acagcctcaa gcacccgcag caattctact taactctcgc
      241 tttaccgcaa accgttttta cccgatatgg gaattcccat atcgtaatga attcagttcc
      301 ctagtcgatc catcaaaaac acaaccaggc agtaaacgcc cacaacagca ataacagcca
      361 gcgcaccttc cattgccagt gaaatatcat ccgacatatt ccctcctttg gtgtgaatcc
      421 cggcgaacgt ttttaccccc accgacaaat aacatatact aaaaaatcaa tagctatagc
      481 aacgcctgta attgcaaagg cttcaggcca gatcattggc gcacctcctg cggcggttct
      541 ggtagcggca tccagtcggt tacattgcgg ctctgtgttt cgaaaaattc atcaccatta
      601 cggactacat caaaaaactc accgtctcga tattgcgcat aaagaacgaa tgcgccatca
      661 cataaaataa ttacgtgctg accatcatcc ggcattcgct cactacagct tatccaacca
      721 tccggagtta ccggatagtt ggttgacgtt tccgagattt cccgaaaatt attggttgac
      781 gaacccttat tttcccgaaa gtttccagcc tgaagcatgg cggcgcggtg acaccagata
      841 atccagccaa gcgccatatc ccataccatg taatctctat cgccattttt tgctctgcgg
      901 cgatctacag attctccgaa acgcttctcc ataaataatt cataggctgc tcgttcatcc
      961 gatactgctg ccagtgatgc cagcgcaatc cgtgccagct cttccgcttc ttctgctgga
     1021 agcacaacgt tgctacccgg tccgtatgtt tcgcgccaat gctggattgt cagcagtcga
     1081 tctttggtaa tagtggtcat agctatttca ccttaatctc aacatttcgc agctttagct
     1141 ctactggcat gtctgacttt cctgttaatg ctaatgcgag attttctgga gtaatgagag
     1201 cagttattgt tttccccatc gccagacgaa taatcattcg tatttcgcga tcgtcacatg
     1261 ctcctggtcg aacaattgat atttgtccgt tcatctcact ctcctttgat gcgaatgcca
     1321 gcgacgcgta atgcgtgttc taggtcaatc aggtaaagcc aactgccatt ttctttaggt
     1381 atcatgacat gtcgctcatc tgcatttatc gggtgtccat atcgaaggtc gtagcgagtc
     1441 ggtaattgaa cttcccgcgc ttccagttca gcaatacgct tgctcccatc agagataacg
     1501 ccttcgtaat actcacgctg ctcgttgagt tgtgattttg cttcttccag tccatccagc
     1561 aaatcagcga taatatccgc ttcccgatga cggatgtgac gcttaaacgc agcaagagcc
     1621 gcatcacaat cccgttcagc atttgggctg tccggggtag cctgatacca cgccagcgtc
     1681 gattgatagt tttgtgctgc ctcacgcagt gcctgatagt caatcttgcc cactggctgc
     1741 ctcctttgcc gggatttcta acttttgagt ggttgtatca aattcaaaca acttaaccac
     1801 gtcatcaaac aggacataat caccatcagg atcttcagtc atatcagcgc cacaatcctg
     1861 accgcacgag tcgcaacctc ccatatcaag ctcgtatcgc ttcaggttcg cgatatttga
     1921 taaattcagc gccagtatag ccagatcatt aacctcgtct gcgatatacc ctgcaccatg
     1981 tccatacatt tcaatgcggg atatgatttc ttctacacgt tgttttgtga tcgtcatttt
     2041 tctcttcact ccgatataca aggattacta caccccctct gctgattgcg cgagctggat
     2101 cccctggttc catgccgtca attccgaagg cttcgaaaaa tgcatccatt gccttctggc
     2161 gttgctcctg cttacggcgt ttattccatt ttttcatgaa caacagtgac agccatcgtc
     2221 cgctgcagaa cataatgtag aaataaccaa gaagtgccag gccggcgttc agggccatat
     2281 caatcgttat cgccgggtca atattcactg cccacctcct gaaaaatcac cgcatggccc
     2341 ggtttctcct ccagtgccag ctcagcccct gcacctgccg actgctgcca gcctttcagc
     2401 atgtaaaccg catccacgca acggagcatt gccatgcaaa tatccatgta gtgtggctga
     2461 gtcagcccgt ccggaagtac tgccgggttt aaaacggtat gcccttcccg tttcagcacc
     2521 tcttccgttt tgtgaaatgc ctcgcggtta aaattttcat atcccgtcat cggaccggcg
     2581 atataaaccc tcaccctcac tcctgaaccc tcctgtcgaa ataaacgtag ttattcactg
     2641 cgcccaactt catcccaaac ttttcggcaa tttcccgtcg gggtacacca cgctgatgca
     2701 gttgccacgc cagctcaata tcacgctgtg aacatttggc tgactggtga taatcacccc
     2761 gtaacatcat gctgataccc agttcccgcg ctttcgtcct gacggctgac tcactacggc
     2821 caatcagata accgatgctt tcgactctca tcgttcccgc acactgccgg agtatcagga
     2881 tttcagccca gcgccacttc ttccagccac tcaccgctgc tgctctctgg tggcggtaat
     2941 atcccggaga atatccctgt gtttgttcag ttcccgtagc gcagcacaga ctcgctccca
     3001 cttctgaaca ttacttttcg cccggcgcag ctcgcggtta gccacatgca gcgatggtag
     3061 aatcaggtca tctgctttcg cttcagtgaa cgattgctgc aacttcacaa tgtcctccac
     3121 cgcttctgtt ttaatttctt cctgtgcagc cgtttcctgg actggtaacg caacaccagc
     3181 tggctgagga agggctttac cctccgtttc agctacggat gctgctttcg gctctgccgg
     3241 taaatcaacg cccggtatgc agtaacgaaa tttaccatcc tgattcacgc gaatcagaca
     3301 ccctttgctg attgccattg ccagcgatga attcgcccgg cgggaggtaa ttccgaacat
     3361 caatgccagt tcatccgccg tttgtgggcc atgttgttca atcgcctctg tcagcatttg
     3421 cgccgtcact ttcggtaccg gtgacaccgg ttcactttca ccagcctgag tcagccacca
     3481 tatcgccccc ttgttatccg cttctccacg gcgtttcagt ttccacagtt cgttgacagc
     3541 ctcttcacga ctaattccaa ggcgagctgc caccacatgt gaagaggctc ttttcagtgc
     3601 tttcagtgcg tcagatacgg tttccattaa aatttcctcc gacaaaatcg tttctcagat
     3661 tcaaacaaaa ccagccgcct tccggcgctc gtattcctgt ttcagtaatt caattggcgt
     3721 tggcccttgc gggcacttcg gcgatgcgag ttgtctccgg atcgggggaa tactcatccc
     3781 gttaccaact tgctttgccc atttcgtcag ttgccgttct gcaagtcgtt ttaactcacc
     3841 ttccgtcatc tgccgctcaa tccctctgtc acgcatctcg aggcaaatgt ggtacagcac
     3901 cggctgtggc cacgggtatt tatcactccc gtcgtaccgc caggattcat tcctccagcg
     3961 acggtactcc tccatcacgg catccaccgt caggccaaat ggatttgccc cgctctccga
     4021 aatcaacgcc acaaactcag ccag
//
LOCUS       DAZFKY010000277         4041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFKY010000277 DAZFKY010000000
VERSION     DAZFKY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..156)
                     /locus_tag="ACHKCL_004602"
     CDS             complement(<1..156)
                     /locus_tag="ACHKCL_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO5765417.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTI"
     gene            400..657
                     /gene="ynaJ"
                     /locus_tag="ACHKCL_004603"
     CDS             400..657
                     /gene="ynaJ"
                     /locus_tag="ACHKCL_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5765418.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(707..1657)
                     /gene="uspE"
                     /locus_tag="ACHKCL_004604"
     CDS             complement(707..1657)
                     /gene="uspE"
                     /locus_tag="ACHKCL_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5765419.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1809..2561)
                     /gene="fnr"
                     /locus_tag="ACHKCL_004605"
     CDS             complement(1809..2561)
                     /gene="fnr"
                     /locus_tag="ACHKCL_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5765420.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2756..3271)
                     /gene="ogt"
                     /locus_tag="ACHKCL_004606"
     CDS             complement(2756..3271)
                     /gene="ogt"
                     /locus_tag="ACHKCL_004606"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5765421.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3282..>4041)
                     /locus_tag="ACHKCL_004607"
     CDS             complement(3282..>4041)
                     /locus_tag="ACHKCL_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFO5765422.1"
                     /translation="SQRFGLRIAGVVSLLFIAAIALMVIPENGILRDPINHTVMPSPF
                     IKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKEMAGFIVMVFPLAQFVA
                     MFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAPIF
                     VPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYSLV
                     LPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1089 a          964 c          970 g         1018 t
ORIGIN      
        1 tatggtgtag attaaaaatt gcaccgcatg gaataaaaag ccttttcttt tacgatttcc
       61 gcggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
      121 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
      181 attgtcgtca tgccggaaac ccctggcggg gctattttac cgcgacaatt cattcagatc
      241 atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa
      301 tccagctaag aggtgaggtt ttcagagcag acaatggtga aatgtcatgg tattgttacg
      361 tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag
      421 tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg
      481 gttgtgactc gcgcgactat cggcggcgtt atagaacagt acaatattcc gctgtcggag
      541 tggacgacat caatgtatgt gattcagtca tcgatgattt ttgtttatag cctggtcttt
      601 actgtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta
      661 aaaaataggc ccgataactc gggccttgtc agttattgag gagtcgttaa tcgtcttctt
      721 cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccaac agatcgcagc
      781 gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc
      841 gtcctaccgt gcccagaacc acaatccccg cctgtaagtg ctccgccaaa tcaggaatca
      901 cctcttctgg cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc
      961 gcagggcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcggat
     1021 caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat
     1081 gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat
     1141 tgagcgcatt atgatacggc tcttcactgg cgagattcac cgccaccagc gccttacctc
     1201 cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacaggt
     1261 gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta
     1321 gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat
     1381 gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt
     1441 ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg
     1501 agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc
     1561 caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag
     1621 gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg
     1681 tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc
     1741 ttctgccaga tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg
     1801 cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc atcgttattt
     1861 tcgatggtga tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc
     1921 agacggctga tggtttctac cgtcaggccc agatagttac cgatatcgcc acgagtcatc
     1981 gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag
     2041 atgaatgcag ccagacgttc ctcggcattt ttctttgaca acagcaggat catgtcctga
     2101 tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta
     2161 ccggacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc
     2221 tgcgcgaagc tcgggtgatg gccgctgccg atggcgtcaa atcccaccag gtcgcctgct
     2281 aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta
     2341 ccggagcgga tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct
     2401 ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg
     2461 agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg
     2521 ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa
     2581 ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac
     2641 agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaaa cccttaagtt
     2701 gttaagggta actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca
     2761 gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc
     2821 cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat
     2881 tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg acggcccaat tgctcagcca
     2941 gttcgccgta atgcattacc tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc
     3001 gctgaaatgg cgtccccccc gtagcagtgg gaagcgtatc aataatgcta agattaccgg
     3061 caaaatattc acgaagcttg tcgcttaaac cgcctggatt ggtggcagaa atgcgctcat
     3121 agccttcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc
     3181 attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggtg
     3241 tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa
     3301 atacccggac ctatcggcag gcccacaaga taccacgcca gcaacatcag cagccatacc
     3361 accaaaaaga taagcggata gggcaagact aacgaatagt aagtacccag tttcgcgtct
     3421 ggtttgtagc gttgcaggaa tccaagaaac agtggaacaa aaggagatac tggcgctaaa
     3481 ggcaatacgg atgagtcggc aatacgaaag aggatttgcg caaatgccgg gtgaaagcca
     3541 agtagcataa acattggtac gaaaatgggg gccagaatcg accagattgc ggaaccgctg
     3601 gcgataaaca tgcataagaa agaggaaagc aacgccagac cgacaaacgc cgggatgccg
     3661 ctaagccctg aactttccag gatatcggtc agccccacgg cgatgaattt ccccatgttg
     3721 ctccagttaa acatggcgac aaactgggcg aggggaaaaa ccatcacgat aaatcccgcc
     3781 atctctttca tcggttcaat cattaaatgc ggtaaatccg cctgacgtcg aattgtgcgg
     3841 gtagcgatgc cataagccag cgagacaaca aagaaaaaaa gaatgatcag tggcacgata
     3901 cctttaataa agggtgatgg catcacggtg tgattaatcg gatcgcgcaa tatcccgttt
     3961 tccgggatca ccatcagcgc aatcgcagca ataaaaagta gcgatacgac acctgctatg
     4021 cgtaaaccaa aacgctgact t
//
LOCUS       DAZFKY010000278         4010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFKY010000278 DAZFKY010000000
VERSION     DAZFKY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>4010
                     /locus_tag="ACHKCL_004608"
     CDS             <1..>4010
                     /locus_tag="ACHKCL_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765423.1"
                     /translation="EVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAG
                     FMDRVSAGASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIA
                     QGSSNVFINNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILG
                     GITIASYLLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIAN
                     KVIRWVTDPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSE
                     YAWVREQGNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSS
                     RFFYDAFPGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRIT
                     GVSHTDGIRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIR
                     WSDNDQTWSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGN
                     ILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYC
                     YDERGLLSNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDER
                     RLGIEYDHHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLE
                     RAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTL
                     QLTEVINPQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYS
                     RDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAI
                     EYHYPDEHTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRD
                     EAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGN
                     VSGVTSREDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSA
                     GRHRISGETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQ
                     LTGYRSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVR
                     HLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPG
                     QTSLWGLALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETG
                     MYLVSDPLGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLG
                     NRALKYANGDWIKSEA"
BASE COUNT          922 a         1129 c         1275 g          684 t
ORIGIN      
        1 ctgaagtccc acagggggaa ccctcatggg gtgatattgc cactgacctg cttgaatcgg
       61 cagcggaaaa agcagtacca ctggcgaagg cctgggggaa cgctgttatc accctgacgg
      121 acagcaatgc cggttttatg gatcgcgtca gcgccggcgc atcgcttctg tttcccgccg
      181 gtccggtatt aatggagttt gccaccatgg tgggcgggcg tggcgaaatc aaaaaagatg
      241 tggatttccc ggaagccggt gaggacacgg cgctctgcga caaggagaac aaaccaccga
      301 ggatagccca gggcagcagc aacgtcttta tcaacaatca gcctgccgcg cgcaagggcg
      361 acaaactgga gtgcagcgcg gcaatcgtgg aaggttcgcc ggacgtcttt attgggggtg
      421 agcaggtcac ctatctggat atccagccgg agttcccgcc atggcagaga atgatcctgg
      481 gaggaataac gatagccagc tatcttctgc cgccagcagg actgctggga aaactgggga
      541 atctggcgaa actgggcaaa ctgggaaacc tgctggggaa aagcggtaag ctgctgggcg
      601 caaagctcgg cgcgttgctg gggaaaacag gtaattcgtt aaaaagtatt gccaataaag
      661 tcatcagatg ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta
      721 ccgactttac gctgggccag acactccccc tctccttcac ccgtttccac agttctgtac
      781 tgccgctgca tggcctgacg ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg
      841 tgcgtgaaca gggaaaccgg gtggatgtca tcagcctggg agccacgctg aacttcgcct
      901 tcgacggtga aagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc
      961 gtgatgatta tctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg
     1021 cctttccggg aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg
     1081 cacacagccc cgcagaccgg atgtacatgc tgggcgggat gagcgacacc gccagcaacc
     1141 gcatcacgtt tgagcgcgac acccagtacc ggatcacggg tgtcagtcac accgacggga
     1201 tccggcttaa actgacgtac cacgccagcg gctacctgaa agccattcac cgcacggata
     1261 acggcataca gacgctggcg acctacgaac aggatgcgcg gctggactac cacctgtttt
     1321 atgagtacga cgctgcggac cggatcatcc gctggtccga taacgaccag acgtggagcc
     1381 gtttcaccta cgatgcacag ggccggtgcg tgaccgtcac cggggcggag ggctattaca
     1441 acgccacgct ggactatggt gacggctgca ccaccgtgac ggacggcaag ggcattcacc
     1501 gttattacta tgatcctgac ggcaatattc tgcgggaaga agcgccggac ggcagcacca
     1561 ccacgtatga atgggatgaa ttccatcacc tgctggcccg ccactcccct gccgggcggg
     1621 tggagaagtt tgaatacaac gccgcacacg gtcagttaag ccgttacacg gcggcagacg
     1681 gcgcggattg gcagtactgc tatgatgagc gcggcctgct cagcaacatc accgcccctg
     1741 ccgggcagac gtggacgcag cagtgtgatg aacgcggcct gccggtgagt ctggtatcgc
     1801 cacagggcga agagacccgg ctggcgtaca cccctcaggg gctgctgtcg gggatattcc
     1861 gccaggatga acggcgtctg ggcatagagt acgaccacca caactggccg gaaacactca
     1921 ccgacgtgat gggccgcgaa caccacaccg aatacagcgg tcacgacctg ccggtgaaga
     1981 tgcgcggccc cggcggtcag tcagtgcggt tgcagtggca gcagcaccat aaactgagtg
     2041 gccttgagcg ggcaggaacc ggcgcggaag gattccgcta cgaccgccac ggcaacctgc
     2101 tggcgtacac ggacggtaac ggcgttgtct ggacaatgga gtacggcccg tttgatttgc
     2161 cggtggcgcg aacggacggt gaaggccacc gctggcagta ccgctacgat aaagacacgc
     2221 tgcaactgac agaagtcatt aacccgcagg gcgagtctta tctttatatt ctggacaact
     2281 gtggccgggt gacggaagaa cgtgactggg gcggcgtggt ctgtcgttac cgttatgacg
     2341 ctgatggcct gtgtaccgcc agggtcaacg gcctagagga aaccatcctc tacagccggg
     2401 atgccgcagg ccgcctggca gaagtcatca ctccggaagg caaaacgcag tatgcgtatg
     2461 acaaatccgg caggctgacg ggtatcttca gcccggacgg cacatcacag cgcaccggct
     2521 atgatgaacg cgggcgggtg aatgtcacca ctcagggccg acgggccatt gaataccact
     2581 accccgacga acacaccgtc atccgctgta tcctgccacc ggaagatgaa cgcgacagac
     2641 accccgacgg atccctgctg aaaaccacat accgctacaa cgccgccgga gaactgacgg
     2701 aggttatcct gccgggggat gagacgctga cgttcagccg tgatgaggcg ggacgtgaag
     2761 tgctccggca cagtaaccgg ggttttgcct gtgaacaggg ctggaatgca gccggtcagc
     2821 ctgtcagcca gcgcgccgga cttttcccgg cggaagccac atggggcgga ctgctccctt
     2881 cactgctacg ggaataccgt tacgacagcg cgggtaacgt atcaggcgtc accagccggg
     2941 aagattacgg acgggaaaca caccgggagt accggcttga ccggaacggc caggtcacgg
     3001 cggtgacagc ctcaggcacc gggctgggct atggcgaagg cgacgagact tatggctatg
     3061 acagctgcgg ctacctgaag gcgcagtctg cgggcagaca ccggataagc ggagagactg
     3121 accagtatgc cgcaggccac cggctgaaac aggccggaaa cacacagtat gactatgacg
     3181 ccgcaggccg gatggtcagc cgcacaaaac accgtgacgg ctaccgccca gaaacagagc
     3241 ggttccggtg ggacagccgg gaccagctga ccgggtatcg cagcgcacag ggggagcagt
     3301 gggaataccg ccacgacgcc agcggcagac ggacggaaaa acgctgcgac cggaagaaaa
     3361 tccgttttac gtacctgtgg gacggcgaca gtattgcgga aatccgggaa taccgcgatg
     3421 ataaactgta cagcgtaagg cacctggtgt ttaacggctt tgagctgata agccagcagt
     3481 tcagccgggt acgacagccg catccgtccg tggccccgca gtgggtgacg cgaacgaatc
     3541 atgcggtgag cgacctgacg ggccgcccgc tgatgctctt taacagtgaa ggtaaaaccg
     3601 tctggcggcc ggggcagacc agcctgtggg ggctggcact cagcctgccc gcagacacag
     3661 actacccgga cccgcgcggg gagcgggacc cggaagcgga ccccggcctg ctgtatgcgg
     3721 gacagtggca ggatgcagaa tcggggctgt gctataaccg gttccggtac tacgagccgg
     3781 aaaccgggat gtacctggtg agtgatccgc tggggttgca gggaggggag cagacttatc
     3841 ggtatgtgcc gaatccttgt gggtatatcg atcctttggg gctggctata tgtcagttag
     3901 cccgctggac gaaatggggg agtgagcaaa gcaacatatc tgatgttttg aactcattag
     3961 ggaatagagc acttaaatat gctaatggtg attggataaa atcagaggct
//
LOCUS       DAZFKY010000279         3981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFKY010000279 DAZFKY010000000
VERSION     DAZFKY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..327
                     /gene="cesT"
                     /locus_tag="ACHKCL_004609"
     CDS             <1..327
                     /gene="cesT"
                     /locus_tag="ACHKCL_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO5765424.1"
                     /translation="MMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEAGAQS
                     LLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNK
                     HYYAGR"
     gene            387..3191
                     /gene="eae"
                     /locus_tag="ACHKCL_004610"
     CDS             387..3191
                     /gene="eae"
                     /locus_tag="ACHKCL_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFO5765425.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3455..>3981)
                     /gene="escD"
                     /locus_tag="ACHKCL_004611"
     CDS             complement(3455..>3981)
                     /gene="escD"
                     /locus_tag="ACHKCL_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5765426.1"
                     /translation="KGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNAKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1170 a          771 c          873 g         1167 t
ORIGIN      
        1 atgatgattt atggtgtctg tgggaaattc ccgacagata actctaactt cgctcttgag
       61 attttgaatg caaacttatg gtttgcagag aatggtgggc catatctgtg ctatgaggct
      121 ggagcacaat cgctgttgtt agcgttacgt ttccctctcg atgatgctac ccctgaaaaa
      181 ctcgagaatg aaatagaagt cgttgttaag tcaatggaaa acctgtattt ggtattacat
      241 aatcagggaa taacattaga aaacgaacat atgaaaatag aggaaatcag ttcaagcgac
      301 aataaacatt attacgccgg aagataaaat ccgatctatt aatataattt atttctcatt
      361 ctaactcatt gtggtggagc cataacatga ttactcatgg ttgttatacc cggacccggc
      421 acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga ttgttttttt
      481 atgttaatca gaattcattt gcaaatggtg aaaattattt taaattgggt tcggattcaa
      541 aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa actggtgaaa
      601 ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg tcgttgaata
      661 agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag cagatcattt
      721 tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt tcggcacctc
      781 ttgttgctgc aggtggtgtt gctggtcaca cgaataaact gactaaaatg tccccggacg
      841 tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa caggcggcga
      901 gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa gataccgctc
      961 ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa cattatggaa
     1021 cggcagaggt taatctgcag agtggtaata actttgacgg tagttcactg gacttcttat
     1081 taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg cgttacattg
     1141 actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct gcaaacatgt
     1201 tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt ttaggtattg
     1261 gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc cgcatgagcg
     1321 gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat ggcttcgata
     1381 tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg atatatgagc
     1441 agtattatgg tgataatgtt gctttgttta attctgataa gctgcagtcg aatcctggtg
     1501 cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg atcgattacc
     1561 gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt tatcagtttg
     1621 ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga acattatcag
     1681 gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac aagaagcagg
     1741 atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt acgcagaaga
     1801 ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat gatagtgcat
     1861 tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa gactaccagg
     1921 ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg gctcgcgcct
     1981 atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt ctgtcgaatg
     2041 gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact tcggctaaag
     2101 cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg gtagctcagg
     2161 ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg gcaaatagtg
     2221 ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg ccaggacagg
     2281 tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt gcggttatat
     2341 tttttgatca aaccaaggcc agcattactg agattaaggc tgataagaca actgcagtag
     2401 caaatggtaa ggatgctatt aaatatactg taaaagttat gaaaaacggt cagccagtta
     2461 ataatcaatc cgttacattc tcaacaaact ttgggatgtt caacggtaag tctcaaacgc
     2521 aagcaaccac gggaaatgat ggtcgtgcga cgataacact aacttccagt tccgccggta
     2581 aagcgactgt tagtgcgaca gtcagtgatg gggctgaggt taaagcgact gaggtcactt
     2641 tttttgatga actgaaaatt gacaacaagg ttgatattat tggtaacaat gtcagaggcg
     2701 agttgcctaa tatttggctg caatatggtc agtttaaact gaaagcaagc ggtggtgatg
     2761 gtacatattc atggtattca gaaaatacca gtatcgcgac tgtcgatgca tcagggaaag
     2821 tcactttgaa tggtaaaggc agtgtcgtaa ttaaagccac atctggtgat aagcaaacag
     2881 taagttacac tataaaagca ccgtcgtata tgataaaagt ggataagcaa gcctattatg
     2941 ctgatgctat gtccatttgc aaaaatttat taccatccac acagacggta ttgtcagata
     3001 tttatgactc atggggggct gcaaataaat atagccatta tagttctatg aactcaataa
     3061 ctgcttggat taaacagaca tctagtgagc agcgttctgg agtatcaagc acttataacc
     3121 taataacaca aaaccctctt cctggggtta atgttaatac tccaaatgtc tatgcggttt
     3181 gtgtagaata attccataac caccccggct aaaatatgta ttgttttagt cggggcataa
     3241 ttatttcttc ttaagaaata acctcttata atcaaatcta ctactggtct ttttatctgc
     3301 ttaataggtc tctttcaaag agacacattc acgttttctc atgtaggttg atccaaccac
     3361 gctgatacca aagctgaatc acatcaagca acaactatgc tcacaacatc cacacaataa
     3421 aaaaccctcc gaagaggggg aagaggataa gaaattaata cgacagtgga atatgtatat
     3481 attcgtctgg gcgggagatg ttgataccat ctttaggatc aatcgcgtct atgacatagc
     3541 ctcctttcag aatgctacca atccccaggc tggtgccatt atcaagagca attgttggat
     3601 tcttcatcgt accgctgatt ctgagtattt ttccggggaa aatatctgct gaaatgtacg
     3661 gctgaatgtt ctgaaagagt atcttggcgt tactattttt gttcattgca tttataatgg
     3721 caaggatctt actctcattc tgttgagaaa cttcccccgc aacaatgatg acatcattct
     3781 ttttgaaaat attgacatag tttattagtt tattcttttt aaactctgat gccagttcat
     3841 tgattttatt gttactgttg ttgtgtattt cccatcctgc aactcctggc gtattcagta
     3901 acagattttc aacctcgctc catttgggtg aagggggaat gtatcccgat aacagaaaga
     3961 atccaggctt attacctttt g
//
LOCUS       DAZFKY010000280         3945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFKY010000280 DAZFKY010000000
VERSION     DAZFKY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..1026)
                     /locus_tag="ACHKCL_004612"
     CDS             complement(100..1026)
                     /locus_tag="ACHKCL_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5765427.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAVSEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1183..2103
                     /locus_tag="ACHKCL_004613"
     CDS             1183..2103
                     /locus_tag="ACHKCL_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5765428.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            2338..3480
                     /locus_tag="ACHKCL_004614"
     CDS             2338..3480
                     /locus_tag="ACHKCL_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO5765429.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT         1203 a          868 c          815 g         1059 t
ORIGIN      
        1 tattaattat cagaaacttt attatgtaaa cgcatcctaa tgattacgtt attcctctta
       61 ccgatattcg cccagagcaa ccatcaatta cacatatcat caattttcga gttttactct
      121 tttctccacc ataaaatcaa taaaagcacg aactttagag ggcattctca cccgtgatga
      181 aacataaata taaaacccag gaaatgaaac agcccaatcc tcaagaatcg gctgtaactt
      241 gcccgcattg atatgttcag cgattgcaaa atccagatgt tgaatgatac ccgttccatt
      301 cagcgccatt cgtatcatgc tttcgtcatc attagaaata tacttacctg aaacctctat
      361 ttcgaactct tcattacccc ccacaacctt agaaaactcc catgcagaga gtgcaccgct
      421 acttttatgc cgcaaccgaa ggcaattatg gttactcagt tcctgaggcg tttcaggaat
      481 accgtgttct tttaaataat ctggcgatgc cacagtaacc agtttaataa gcggagaaac
      541 aggaacggca gtcatatgct catccagtcc ttgctcaaga cgtatgccca catcacagcc
      601 gtccgcaata atgttagata aaccgtcatc cataatgagt tctatctttg ttttggggta
      661 acgagtaaga aactcgccaa tatgaggctc aacaagcatc cgtgctgcaa cacgggagga
      721 atttattctc aacaggcctg taggttcaag ttgggtatca ccaacatctt tcaaagcatc
      781 atcaatttgc cccaaagccg acaccagcac ttcatacaga cgctgtcctt cctcagtaag
      841 cgagatattg cgggtcgtac ggtatatcag cttggtgtta agacgttcct caagagattt
      901 taaattctgg gacaatgcgg ccagcgtaat acccatttca gaaacggctc tggaaaaact
      961 gccggcacga acaatcagcg caaaccaggc aagagaaggc aaaattgaag gctctatttt
     1021 catcattgta aaccttaact taacactgtg atagatatta gaccattgat gataaccaat
     1081 acaagtcata ctattacgcc gttctcgata tgaacacaaa tgccagttgc ggataataga
     1141 aatgatattt aaatctgttt aggcaaatcc ccggagcttg aaatgaaaac tgtttcgata
     1201 aaacacactt actgggatat agctgctgat atctatttcc ctgacgattt taatagtgaa
     1261 aaaaagtatc ctgccattat cagcgcccat ccaattggaa gctgcaaaga acagacatcg
     1321 ggtagtgtgt atggtgccgc cctggcaaaa gctggcttta tcgtcattgc ttttgatgct
     1381 agttttcagg gaagcagcgg tggggaaccg cgttatctgg aagatccaac aatgcgagta
     1441 aaagacttca gcattgtcgt tgattacctt accagcttac cttacgttga tgccgggcgt
     1501 attggtgtac tgggtatttg tggtggaggt ggatatgcca ttaatgccgc aatgacagaa
     1561 cgccgaatta aagctattgg tactgtcacg ggtgcaaact acgggcgtct tatgcgtgag
     1621 ggatttacag cgtttaatcc tatcggagca cttgaagcaa tggcgcaaca aagaacagac
     1681 gaagctaacg gcgcaaaact tcgcgtagat gatttactcc cctcttctcc ggatgcagct
     1741 cttgaagcgg gactaacaga aatagacctt tttgaagcca cagaatatta tcgttcgcca
     1801 cgaggctatg cacctaatgg ggtaaatcgt tcgctattct cacatcagac tgttgccgtg
     1861 ggttgggatg catttcatct tgcagaagtg ctgttaactc aaccaataat ggttgtggtg
     1921 ggcgatagag tgggcgcatt tggtgcctac cgtgacggct gcgaaattat tggccgcgct
     1981 gcttcaaaac ataaagagct ggtcgtggtt gaaggttatt cacattacga cctgtatgat
     2041 aagccagaac ctgtgaaaca ggcactcgat aaattaattc ctttttataa aacacatttg
     2101 taataatttt acagggagat cccttatctc cctgacattt atcctttatt gactacacaa
     2161 caataataaa attattcaaa aagaaatttc attaaaaaac aaaaaagcag caactcaatt
     2221 attagaaacc ctttaaaggg attataaata aagccagaac tcttggttcc aaagctggca
     2281 acaactaacc agttaaaaca tacgcatcaa aatcagataa acatttgagg tattgttatg
     2341 acaaataaac acccatcatt gttttctcct tttatgctga cagaaaaaat aaagctacgt
     2401 aatcgtatcg ttatggctcc tatgacgacc tggtcagcga atcctgatgg aaccatctct
     2461 gagcaagaac tcgaatttta taaacgtcgc tcacaaaatg ttggtttagt aataacagga
     2521 tgtacctatg tcacgccaag cggcattggg tttactcatg aatttgccgc gtatgacgac
     2581 cgtttcatta acagccttga aaagttagca gcggcagcca aaagtggcgg tgctccggct
     2641 attttgcaga ttttccacgc cggtaataaa gccatcccgg agcttgtacc aaataacgat
     2701 gttattagtg ccagcgcatc aagcgtaaaa tctggtgact ttatgaagag agtggtacaa
     2761 agcagggaaa tgactgaaaa cgaaattcag gaaaccatta gagcattcgg agatgtcaca
     2821 aaaagggcga ttaaagcagg ttttgatggc attgaacttc atggtgcgca cggattcctt
     2881 ctacagaatt ttttctcccc tctatttaac cagcgtaatg accgatgggg aggagatctc
     2941 gaaggacgta tgcgcttccc gctggccgta ttacaggaag taaaaaatgt agtctatgaa
     3001 tatgcgacaa agccctttgc catcggttac agaatatctc ctgaagaatc tgcaacaggg
     3061 gggctgcgga tagaggatac ttataaactc cttgatcggc tcatttcatc cggtatttcg
     3121 tatatacata cctcgctggt cagtattaac gatagctacc ctgttgagtc gccaaatggc
     3181 ccccgaacta ttgaattgat tttaaatcat attgcaggca gagtgccggt aatcgctgca
     3241 ggtaaaatac gcacaccgtc tcaggctcaa gaagcaattt ccactggcct gccacttgtt
     3301 gcgatcggta aagggctggt gataaatcca gaatgggtga ctttagctga atcaggtaga
     3361 ggccatgaaa ttcaaacaac ccttaatcca caacttgttc ctgaattaac cattccggat
     3421 aaactatggg atcaaattca ggcatcgaaa ggaactggct ggtttccgtt aatggattaa
     3481 atatctatta ctctcccgag agtaaaacta tcaaatcaat gagtaaccga tacagcctca
     3541 caaattcttt cagataatta acccgtcact gcggcacgtt ctctcataag gcgtgcctgc
     3601 agtgtaatct agtatgtatc cggcatagtt gacggtttaa tagagctcaa ttcctcgccc
     3661 aggttaccaa caacgatatc ttgtataaca ccccaggaga tacgacatta tgatgagatg
     3721 cgatatgctc ctcagcgcca atcaacgcca ggactgggcc agtcttgaga tcagactcat
     3781 tcccggaact gacttcaggc gacatatatc aatgacgttg gggcaataaa agatgcctga
     3841 tgtaatacat ctattcaaat ttgtgataca tgtcaaaaaa ttggttgacc aaactcgtta
     3901 tttatataag ggcacttacg aagtgcgctc ttttttaaag cgagg
//
LOCUS       DAZFKY010000281         3925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFKY010000281 DAZFKY010000000
VERSION     DAZFKY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..606
                     /locus_tag="ACHKCL_004615"
                     /pseudo
     CDS             1..606
                     /locus_tag="ACHKCL_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(849..1544)
                     /locus_tag="ACHKCL_004616"
     CDS             complement(849..1544)
                     /locus_tag="ACHKCL_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5765430.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTLLPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1537..2964)
                     /locus_tag="ACHKCL_004617"
     CDS             complement(1537..2964)
                     /locus_tag="ACHKCL_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5765431.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2975..3694)
                     /locus_tag="ACHKCL_004618"
     CDS             complement(2975..3694)
                     /locus_tag="ACHKCL_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5765432.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1024 a         1036 c          822 g         1043 t
ORIGIN      
        1 attcttgtta tcctttttat tatagctatc tgtatggcaa tggtgctttt atttctgtat
       61 atcaataaag ataatataaa agtgagttac agcctaaaaa taaatcagac aacacccggc
      121 atacttgtta actgtgatag caataataac tttgcctgtc agacaacaat gactgaagat
      181 gttattcagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      241 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      301 gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc ataatggcag gtaagttctc
      361 gatgagatac aaacgccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      421 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      481 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      541 atttctgtaa tgttttgtct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      601 cagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      661 gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      721 agtaatgcct ggatatttac agaagaggag cataaccatg acggaacggg gttaagtcag
      781 gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt ccagacttca
      841 tacctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      901 acgactttga taagctcaat gtccgccgtt gagctgggac cactgatgat gttaatacat
      961 gacggcatgc gttcaccggc ctgcgctttc tgatgcaatc tttctgcgag ttgcgctaca
     1021 cgcggcagga gagtgctttt acgcaggata aaaagagatg attccgggag caggctcaat
     1081 gaacgcccac gctcggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1141 tcagcataca cgacacccac tttagcctgc tctgcctgcg agatattctt ggcacctttc
     1201 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcgctaat ccctaattcc
     1261 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1321 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1381 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1441 ttagcatagt tattaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1501 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1561 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1621 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1681 gcttgccgca tgagcaccgg ccatcatccc gactttccac aatcctggat gactattggc
     1741 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1801 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgttgtc
     1861 acaagctgtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1921 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1981 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     2041 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2101 ctcttcagga ccatcaacgt gccccgcttc gcgaggtcct gtcagccagg tgttgtatcc
     2161 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2221 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2281 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2341 gaaattacag cccgtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2401 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2461 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagagggcgg
     2521 atcttgatcc agctggagaa tatattcacc caaatcggtt tcaatcacct gaatgccagc
     2581 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2641 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2701 ttttgcaaaa tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2761 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2821 ccagagcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2881 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2941 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     3001 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3061 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc gcaacctttt
     3121 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3181 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3241 taagtggctc gtccttcacc cccagcttac gggccaggct acaagatggg tgatacaccg
     3301 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3361 tgagatcctg catacgcgcg gcaacctttt cggctcgtga tgcccattca ggttcatccg
     3421 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3481 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3541 tataaccact attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3601 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3661 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3721 atttattccc gatgtttccc cccacccggg gatcaatcat caataaatat ggcgttcttt
     3781 tttaattggc tagcaacata ccacccctta tttgtatgac aaatgatagt ctttcatcca
     3841 aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt tatggggaaa
     3901 tcgacgatca tttttggcat aagaa
//
LOCUS       DAZFKY010000282         3925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFKY010000282 DAZFKY010000000
VERSION     DAZFKY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..821
                     /gene="nanC"
                     /locus_tag="ACHKCL_004619"
     CDS             105..821
                     /gene="nanC"
                     /locus_tag="ACHKCL_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5765433.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            841..1947
                     /gene="nanM"
                     /locus_tag="ACHKCL_004620"
     CDS             841..1947
                     /gene="nanM"
                     /locus_tag="ACHKCL_004620"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5765434.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2012..2992
                     /gene="nanS"
                     /locus_tag="ACHKCL_004621"
     CDS             2012..2992
                     /gene="nanS"
                     /locus_tag="ACHKCL_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5765435.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            3115..3810
                     /locus_tag="ACHKCL_004622"
                     /pseudo
     CDS             3115..3810
                     /locus_tag="ACHKCL_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1099 a          901 c          940 g          985 t
ORIGIN      
        1 ccaaagacac tataagcctg gcaaaaaaat attacacaac ataaatgcta attgtttatg
       61 cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa
      121 tactttctgg cgtattatta ctgtgctttt cgtcaccatt aatttctcag gctgcgacac
      181 tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga ctcaaagtca
      241 gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg aataccattc
      301 atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt aattacgcga
      361 ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg
      421 gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat cttaattttg
      481 gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc ggtgatatgt
      541 ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat agtgatttca
      601 ccttcgcatg gcaaacgacg ctatacagca aacagaacga ttatcgctat gcaaaccata
      661 agaaatgggc gacggaaaat gcatttgttc tacaatacca tatgacgccc gatattacgc
      721 catacataga atatgactac ctggaccgtc agggtgttta caacggcaga gataatttat
      781 cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga gacagtcaca
      841 atgaataaaa caataacggc gcttgctatc ctaatggctt catttgccgc aaacgcgtct
      901 gtattaccgg aaactcctgt accatttaaa agtggtaccg gagcaattga taacgacact
      961 gtctacattg gtttaggtag cgcaggtacg gcatggtaca agctggagac acaggccaaa
     1021 gataaaaaat ggacagcgtt agctgcattc cctggtggac caagagatca agcaacctcg
     1081 gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag cgagggcttg
     1141 actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg ggttaaattg
     1201 atatcgcacg cgccgatggg catggcgggc catgtgactt ttgtacacaa cggcaaggct
     1261 tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga tctcaacgaa
     1321 gctggaaaag attcaaccgc tgtagataaa atcaatgcac actattttga caaaaaagca
     1381 gaagattatt tctttaataa gtttctgttg tcttttgatc cctctacaca gcaatggagt
     1441 tacgctggcg aatctccctg gtacgggacg gctggtgcgg cggttgtgaa taaaggtgat
     1501 aaaacctggc ttattaatgg cgaagccaaa cccggattgc gaacggatgc cgtatttgaa
     1561 cttgatttca ccggtaataa tttaaaatgg aataggcttg ctcccgtctc atcaccagat
     1621 ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc cggaggggcc
     1681 ggattcaaag gttcacgaga aaattaccaa aacggtaaga actatgcgca tgaaggcctg
     1741 aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga taaatcgggt
     1801 gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag tctattgatt
     1861 attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttgat ctctgtgaag
     1921 gataataaag tcacagtaca aaactaacgc ttcagggccc cggtaaaggg gccctgctat
     1981 caatttgtca tttcaattac gcgaaattta tatgaacgca ataatatcgc ctgattatta
     2041 ctatgttctt actgttgctg gtcagtctaa tgccatggcg tatggcgaag gactgccatt
     2101 accggacagg gaagatgcgc ctcatcccag aattaaacaa ttagcgagat ttgcgcatac
     2161 gcatcccgga ggcccgtcat gtcactttaa cgacattatt ccactgactc actgcccaca
     2221 cgatgttcag gatatgcaga gttatcacca tcctctggca acgaatcatc aaacacagta
     2281 cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgcccttca ttcctgataa
     2341 tgcagggatt ctcatcgttc cgtgttgccg tggcggatcg gcttttaccg caggcagcga
     2401 ggggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac
     2461 tccgctatac caggatttag tcagcagaac gcgagccgca ctggtaaaaa atccgcagaa
     2521 caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc
     2581 gtcacatcct caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaata
     2641 ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata ccacctggta
     2701 ctggaaagaa aatttccctc atgcgtatga agctatttat ggcaattatc aaaataatat
     2761 tttagctaat attattttcg tcgacttcca gcaacaaggt gcaagagggc tgacgaacgc
     2821 gcctgatgaa gatccggacg atttaagcac gggatattac ggttcagcgt accggtcacc
     2881 ggagaactgg acgacggcac tgcgaagcag tcatttcagc tcagcagccc gtcgggggat
     2941 tatttctgac aggtttgtag aagcaatttt gcagttttgg cgcgaaaggt gagcgttcat
     3001 taatttgatt tattcgcatc catcagcgct tttaactcct ccggcgtgcg gcactataac
     3061 gacttccaca acaaggagcc attttcttat accactttca gtgaccatcc gcttatgcca
     3121 ccaccctcta cgcaactgtt tgcagagcac cttcccaccg agtggataca gcacttcctg
     3181 acgctttctg ctcatgcgac cgttcgtcgt cttaacctga gcgtggatgg cgaagcgggg
     3241 atgaacctgc tggcgcccgc agcgctgtca cccaggcgcg tcagcgcgtg ggggccgccc
     3301 cggtggaatg gctcttccgc cagaccacac agacatgggg cgcggaacgt tacctgaagg
     3361 atgactggca cggcctgcaa ctttttgcca ttgatggcgc acagttcagg acacctgatg
     3421 aacccgagct gcgtgaatat tatggctctg ccaacacatc cactgagcgg cagagcgcct
     3481 accccgtcat gcgtctggtg gcgttaatga atctgggaat cacattctac tgaatgccat
     3541 gaccgcgcct accggcggag cgatacggcg tgaggcaaca aaagcggcgt cagagatcag
     3601 ctttaagttc gcgttccagt ttatcgtgac agaaatgata gtgctgggaa acacggcgtc
     3661 accggggact atcccgaaac gactggagca tctgcggggg ctctggaagt ggtgttcata
     3721 acaaaacgcc cccgaccatc aagggcgagg gcggttaaga tatcaaaaac ccgttatcct
     3781 gtgaaacaca gtgccgcccc gcttaagtga acggcattgt gtttctgagg gaccttaatt
     3841 ttccctgatt ctctgacgtt ccttaccatt gatatttcct tcattcctca ccagagaaat
     3901 aaaaaatcac tatactcatc aaaat
//
LOCUS       DAZFKY010000283         3909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFKY010000283 DAZFKY010000000
VERSION     DAZFKY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..917
                     /locus_tag="ACHKCL_004623"
     CDS             <1..917
                     /locus_tag="ACHKCL_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5765436.1"
                     /translation="ANETSFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEK
                     LKVKIESLGKTSDTIGKSSNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIGG
                     ESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIKV
                     DDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTIL
                     RGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIPV
                     MASYYVYDEKKVKSGALEATALINVKYD"
     gene            complement(999..1337)
                     /gene="rcnB"
                     /locus_tag="ACHKCL_004624"
     CDS             complement(999..1337)
                     /gene="rcnB"
                     /locus_tag="ACHKCL_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5765437.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1555..2406)
                     /gene="rcnA"
                     /locus_tag="ACHKCL_004625"
     CDS             complement(1555..2406)
                     /gene="rcnA"
                     /locus_tag="ACHKCL_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5765438.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEYQD
                     AHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATL
                     VVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVH
                     GFMGIMR"
     gene            2527..2799
                     /gene="rcnR"
                     /locus_tag="ACHKCL_004626"
     CDS             2527..2799
                     /gene="rcnR"
                     /locus_tag="ACHKCL_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5765439.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2909..3223)
                     /locus_tag="ACHKCL_004627"
     CDS             complement(2909..3223)
                     /locus_tag="ACHKCL_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO5765440.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3233..3580)
                     /locus_tag="ACHKCL_004628"
     CDS             complement(3233..3580)
                     /locus_tag="ACHKCL_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765441.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1119 a          786 c          891 g         1113 t
ORIGIN      
        1 tagcgaatga aacttccttt agtaaaagtg tcgtgtttac tgggatatct tgtgatacga
       61 gcacggataa aatagtttat aaaaatatcc aaagtgattg ggttgaagtt gggccttttg
      121 gtaatggcga aaaattaaag gttaaaatag agtctttagg taaaaccagc gacacaattg
      181 ggaaatccag caatgcgcag gcagtattac cttatgtggt taaaatagcc agaggcacac
      241 ctgattttac tggagaaaga aaatctacct ggtttatttc agataccgtg attgcaaata
      301 ttggcggtga gtcatcgtca tccatcgatt tttggttggg tatttgtaag gcattgaagt
      361 ttaactggtg tgtgaattat ctcaccagca aactggcggg ggatacattt acgcttgggt
      421 taaatatttc ctattatcct aaaaatacga cctgtaagcc tgaaaacacc gttataaaag
      481 tagatgatat cgccttgttc cagctcagaa atcagggaaa gattgcggcg aacagtaagg
      541 aaggaacaat tacgttgaaa tgtgataatc ttttcggcga caaaaaacaa gcatcgcgga
      601 atatggttgt atatctttct agcagtgact tagttaaagg aagtaatact attttgcgtg
      661 gtaaaacaga taatggtgta gggtttgtgt tggatctaac agaaccacca aaagggactg
      721 aggctgccat taaaatttcg gccaacggcg atcagggcgc ggcgacatca ttatggaaaa
      781 cagataaacc aggagtttca ttaaatagca acattattaa tataccagtc atggccagtt
      841 actatgtata tgatgaaaaa aaagttaaat ctggcgcact ggaagcaacc gcattaatca
      901 acgtgaaata cgattaataa caagcaagat gagcaagtgg ctaataataa gaaataataa
      961 ggtgtctgca tttccccctc tctccgaggg ggcttttttt agcgatgata aaaaatctca
     1021 ccgtcgtagg ctttaatgat tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc
     1081 atataggtcc agtgcgtccc ggcatccggc gcgggcaggt tacgcaactg ccactgctta
     1141 atgttgtact catcggtacg gtacatctct ggtacggtgt cccctggttt gtaatgctta
     1201 aagtcagcaa tgaaactgct taactcatac ttagaaattc ccgaggtatt ggtcgaaccc
     1261 gcggtggctg gtgcggccca ggcggcactg gtaaccagca aaagcgcacc cagcaacatc
     1321 ttatttttaa tggtcataaa ctctccactt tggggatctg taatatcagt cgcatatctt
     1381 tcctgccata caatataact gacaagcgat attttgttag tgatcccatg atttcgtaaa
     1441 aagtgtaaat gaaaggatgt agcaaaagca tcctcattga gcgagaagtg agcggaataa
     1501 gctgatttaa gaatcatcct gggctaaaag gatagcgaag cccaggtgat tgttttatct
     1561 cataataccc ataaagccat gcactcccat atacaccccg actaaaccaa ttaacagact
     1621 ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg ctccagcgtt ttgcaacttg
     1681 ttgaacactg attgccgctc caacgcctac tgtaaccagc gttagtgcca gaccgatgct
     1741 gaaactgacg accagtgttg cgcccagtgt cagggctttc aactgaatgc aaatcaacag
     1801 cacggtaatt gctgccgggc agggaataag gccacctgtt aagccaaata acaaaatttg
     1861 ccagttggtg acctctctac catcaaagcg tcgtttaatg tcattggcat gggctcgtgc
     1921 atgggcatcc tgatactctt cgttgtcgcc atgttcatga tggtgatgat gttcgtggtc
     1981 gtggtcgtgg tcgtggtcgt ggtcgtggtc ttgatgatgt tcgtgatcgt ggtgatgatg
     2041 ctcatgcata ttctccagcc agttgcgttc gccgcgccag gtacgccaga acatccagaa
     2101 agcggtgcta ataatgatca ctgcggaaat caactggagc cacggttctg ctgattgagc
     2161 agtaaagcgc ttgctgatca ccatcccgcc aaaggcaatc aaccagacca ctgcggtatg
     2221 cgaaatggtt gctgccagtc cgagcatcac cgcctgttta atggtgcctt tgatggcgat
     2281 gataaacgcc gccatcatcg tttttgagtg ccctggttcc aggccatgaa gcgcaccaag
     2341 taagatggcg ctggggatga agaaccaggc gtttccttgc tgaagaagag ttgtaaattc
     2401 ggtcatgata ataattctta gtattaattc ggcaatctga ttctactccc ccccagtacc
     2461 tgatactacc ccccagtaga ttaatagtgc tatgattttt catgttcttg ttaaccaggt
     2521 gttgccatgt ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt
     2581 cagggccagg tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt
     2641 ttacaacaga ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga agtgattaaa
     2701 ggtcatctaa cggaacatat cgttcaccag ggggatgagc taaaacgtga agaagatctg
     2761 gatgttgttc tgaaggtgct ggattcttat atcaaatgaa agtgcctaat atggccgtag
     2821 ttgaggcaat aaatagaggg acaagtgacg ttaagctgaa ttagagtaaa tggcgtgagg
     2881 acacacgcca ttcgaggtta tagtttgatt aattttcgaa aaattgcttc acagcccagc
     2941 caccatgacc acctggagtt atgaatgaaa caaaaccttt gtcatctacg ctctggattt
     3001 ctaccgtcat tgattcatct aacacgcgac atttattgga tgtaatgaga ctcagcatag
     3061 cctgttggtc attgtcctgg atgtaactga tcatctcgta aaagtcagtt aactttttac
     3121 agttaaatgt accttcttta agtgttgcgc tgtctccgtg acttagtgcg aatgcatttg
     3181 gcatcattgc cacaaagatg acggtaacga gtagtttttt cattttagcc tcttataatt
     3241 cagaacattc actgatgttg taagtgcctt ttctaccatc atcgtttacg agatgtagca
     3301 cgaagatgat gggattggca tcaatgaaag atacagagac gttcccgctt tcggtgctat
     3361 acatctttgc aatctgtttt tcaccaatat atgagagatc ccaactcact ccgttcaagc
     3421 taaaatgcat ctgtttgccg tcaatatcga tggaggattg tgccgtaacg gttttcccat
     3481 tgctgccaat ttgaccctgc tgaagagcat cacatttata ggagcttgca taagtagata
     3541 gaggtagtag cagaagtgca gcaaaaattt tactcttcat attttccctt tgcttggatt
     3601 tgggttacct attttggggt agtcattata aaactattaa ttagtaaaac gaacaagttt
     3661 tcatttgttt agcaatggta ttgtttaggt ttgaaattat tacgtatttt catagcaata
     3721 ggttggataa acagcagttc agggaacaag ttgcactatc tacatacttt tccgctttcc
     3781 attgcatgtt aggtaataga tagcctttgc ccgtaagact aaaaatatta gaaatgactc
     3841 attcgaatac cttctattca ccttaatata ggtttgatta ttgtaatcgt ttaatgtgtc
     3901 ttatgattt
//
LOCUS       DAZFKY010000284         3882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFKY010000284 DAZFKY010000000
VERSION     DAZFKY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..284)
                     /gene="escO"
                     /locus_tag="ACHKCL_004629"
     CDS             complement(<1..284)
                     /gene="escO"
                     /locus_tag="ACHKCL_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO5765442.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVE"
     gene            complement(344..1684)
                     /gene="escN"
                     /locus_tag="ACHKCL_004630"
     CDS             complement(344..1684)
                     /gene="escN"
                     /locus_tag="ACHKCL_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO5765443.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1668..3695)
                     /gene="escV"
                     /locus_tag="ACHKCL_004631"
     CDS             complement(1668..3695)
                     /gene="escV"
                     /locus_tag="ACHKCL_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO5765444.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3692..>3882)
                     /gene="cesL"
                     /locus_tag="ACHKCL_004632"
     CDS             complement(3692..>3882)
                     /gene="cesL"
                     /locus_tag="ACHKCL_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO5765445.1"
                     /translation="NAFFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLM
                     NTFQQHVQIIERVRRMTS"
BASE COUNT         1188 a          895 c          708 g         1091 t
ORIGIN      
        1 ttttctactg atttattaac atgttgcaga ttctcttttg ttcgtttttg tgaatctagc
       61 aatgaacgct tttccttcga aagtgaagat tttttctcat acaatcgctg cttttgctca
      121 tcaaatgcat tgtttttaat ctgatattta tacaacgaca ctttatccaa attcaccagc
      181 gatgcacaat atgcctgctt agaggcaatc gactctttaa tggattgctc gcagtcatcg
      241 agtttaccat ccacctcttt tatctgagag ttaatcttag ccattgattc tctcaatcgg
      301 ttcgctctgc ttttacgaat agataaaatt ctgtccaaca tactcaggca accactttga
      361 ataggctttc aatcgttttt tcgtaactac tgatatcttt ggtgctttgt tgtataaagt
      421 tctgaattaa tttcctattt tttattgcct tatctgcttc aggatcttgc cccatcgtgt
      481 actcaccaat acgaataagc agctcaacat ttttataagt tgcaataagc tttttgcatt
      541 ctgccgccgc acgcaaatgc tccgatgtaa caacgttatg cataacacga ctggcactta
      601 aaccaatatc aattgcaggg aaatggtttt cctctgcaag ttctcgggta agtacgatgt
      661 gcccatcaag aatagagcgg acttcatcgc caataggatc attaacatta tcgctttcta
      721 acaacaccgt ataaatcgcg gttattgaac cttttggcgc aggccccgct cgctctaata
      781 atttgggtaa cgaagaaaaa acactcggag ggaatccccc ccttacatca ggttctcccg
      841 atgcaagccc aacatcacgt gcggctcggg cataacgagt tacagaatcc atcattaata
      901 atacattttt accttgatcg cggaagtact ctgcaatagt cgtcgccgta aatgcggctt
      961 tcatcctttc cagcgcgggg cggtctgacg ttgtgacgac cagtacacat ttagaaagcg
     1021 tggattgagg taagagcgcg aggaattcat ttacttcgcg accacgttcg ccgataagag
     1081 caaggacaat aatatctgcg gatgcgccat tacaaatcat ccccaaaagc gtacttttac
     1141 caacgcctga accagcaaaa ataccgatac gctgcccaat accgcaagtt agtagcccat
     1201 caattgccct gacgccaagt ataaatggct gatcaataac ctgccttaat aaaggatctg
     1261 gtggttcagc atacaagctg cgttcaaacg gaagataggg ggcagcaata ttactctcca
     1321 ttggtctgcc tatgccatca acaagccgcc caagtagtgc gtctccaaca cgaatcttga
     1381 actcatctcc ctgataactt aaccactgac cacagtacat gccggaaaca tgctcaaaag
     1441 gcaacaagaa aacctcatct tcatcaatag cgatcacctc ggctaaacgt tgactgggtt
     1501 ctatcttata aaaggcgcca atacgcgctt ttggtaaacg cgccttaata atcgtcccgc
     1561 cgatattgat aatcttgcct tcatatctgg tagacgaatt taatgataat gccggcacag
     1621 tgctattgag cactttctga atacgtgggt atttttccaa tacagaatca tgctctgaaa
     1681 tcatttaccg ttcctaatac tttaagttcg atattattcc caacctcctg aaaagagagc
     1741 acggggactg acgggaacga accttcaatc attttcttca catagcgcct gatatctaaa
     1801 gcggtcagaa tgacgccgtt ccccgttgga ttcatgatat tttttaagaa gccaattatc
     1861 tgctcagttc gctccggtga gatattcaga tacgaacctg atgacgtttg tctgatcgac
     1921 tctcgtagct cattttcaat atcagagcca ataagccaaa cgttcagaag tgtaccgcta
     1981 acgctatagc gacctaaaat atgccgacgc agggcaatac gaacgtattc gcacaagata
     2041 accacatcct tttcttttgt tgaccaaaaa ataagcgtct caaagatagt tctcaggtct
     2101 ctaattgaga cattttcctc tacgagacgt tgtaggatgt caacaatttt gctcaaacca
     2161 agctggcgct gcagctcttt cacaagctcg ttatattttc tctccatgat gtccatcaaa
     2221 taacgcgttt cttgtacgcc gatgaactct ttggcattag acaatacaag tttctttagc
     2281 aaaaacgtta tcttatcatt tgttgttgca taaacatcca tacctgtaag gtgagcgagc
     2341 tgttgattag tcttattcgt cttatatacg atagagttag tcgaaagctt ttcctggaca
     2401 aattcgaatg atatctctgc atgcccagcc tcaaaataga cagtatcatc tataagagta
     2461 tctttgtata ttgactcttg atagagcaaa aattctattg cgctatcatt atttttaacc
     2521 ggattaacaa taatcttagg caatggaatt ccactttcct caaagaaatt ccaacgcatg
     2581 acctcaattt gctgtgtaat gtctgaacta taaatattac tacttaagtt taaaatcaat
     2641 ggctcagcgc ccggtgagat gttttggcta ttagacaaat cagctcccat agcatcctta
     2701 ttattgccag ttccaaatgt tcttttcttc tgtaatttcc aggctatccc caacaggcaa
     2761 accgccagaa ataagaagac caatgcagga aagcccggaa taatcgccat tacgagcatt
     2821 agtgcggcac tgattaaaaa agggcgacta ttaacagaaa cctgttgaac aatatcacct
     2881 gcaaggtttt cttctttttc gctttcaccg ggcacacgag taacaaccac gcctgcggtg
     2941 acagaaataa taagcgcagg gatctgggca accaaagcat caccgacaga caatacagaa
     3001 aacaggctaa gtgccgcact aaatggcatg tcaaattgcc acataccaat gagcacgcca
     3061 ccaaaaaggt taactaaaac aataatgata ccagcaatag cgtccccttt tacaaacttc
     3121 atcgccccgt ccattgaacc ataaaactgg ctttcctttt gcactcggga gcgaagaact
     3181 tttgcctcaa gcgggtcaat aacgcctgcg cgcatatcac catcaatact catttgcttt
     3241 cctggcatac catcaaggga aaaacgagca ctcacttcag caacacgctc ggcccctttt
     3301 gtaataacca taaattgaac aatggtgata atagtaaaaa taaccaggcc aacaacaata
     3361 ttaccgccaa caacaaagtt accgaaggag taaataatgt cacccgcatc gtgatgcagc
     3421 agaataagtc tggttgtact aacactgagc gacaggcgca tcaacgtcgt aatcaacagg
     3481 attgtgggga aagaagttag ttcaagagga ttttttatat aaattgagag cattaataaa
     3541 agtaaagctg tcgaaatatt aatcgcgata atgatatcca ccacaactgt tggtagtgga
     3601 ataatcatca tcattacagc cataaagaag aagagagcca gaataagatc gtgatatgac
     3661 tctgcttttt taaatatatt taagagttta ttcatgatgt catcctgcga acgcgctcaa
     3721 taatctgcac atgctgttga aaagtattca ttagaaaaaa aacatcgata ttggttaaca
     3781 aacaacttaa gcacaattta ttatcgtaga caaaaatatg ttcaatcact ccctgcgtac
     3841 gctcaggagc aagggcacta aagaaagaga aaaatgcgtt tt
//
LOCUS       DAZFKY010000285         3799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFKY010000285 DAZFKY010000000
VERSION     DAZFKY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..143
                     /locus_tag="ACHKCL_004633"
     CDS             <1..143
                     /locus_tag="ACHKCL_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765446.1"
                     /translation="MLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAMAR
                     IK"
     gene            483..1301
                     /locus_tag="ACHKCL_004634"
     CDS             483..1301
                     /locus_tag="ACHKCL_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5765447.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1392..1877
                     /locus_tag="ACHKCL_004635"
     CDS             1392..1877
                     /locus_tag="ACHKCL_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5765448.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1893..2369
                     /locus_tag="ACHKCL_004636"
     CDS             1893..2369
                     /locus_tag="ACHKCL_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5765449.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            2432..2653
                     /locus_tag="ACHKCL_004637"
     CDS             2432..2653
                     /locus_tag="ACHKCL_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5765450.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2816..3190
                     /locus_tag="ACHKCL_004638"
     CDS             2816..3190
                     /locus_tag="ACHKCL_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5765451.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3237..3611
                     /locus_tag="ACHKCL_004639"
     CDS             3237..3611
                     /locus_tag="ACHKCL_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5765452.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            3608..>3799
                     /locus_tag="ACHKCL_004640"
     CDS             3608..>3799
                     /locus_tag="ACHKCL_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5765453.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFA"
BASE COUNT          893 a         1054 c          975 g          877 t
ORIGIN      
        1 cgatgctggc gatggagcat atccggaaac cgctggcagc acgtcaggca ctgacccggt
       61 cagaggcggt attgcagaaa agcctggata taacggaaga ggcagatttg gcactgagaa
      121 acgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt
      181 ttttcctgtc tggtgttatt gaaaatattt tcagatatat attatctggg tgatggttaa
      241 gatgaattta tttaattttg ggggaagata caaaaacaac cagtgaacat atttccggcg
      301 ggttatccgc aatgctgaag gtcagttgca ctggcgatgc cggaatacag cttaatacgt
      361 ggttcgccag tgaagggctt ctcagacaat aaacctcatt catttcaccc atcaggccgc
      421 gtcttctccg ggagacgcgg ctttttcatt tatatcgctt aatcattcat aaggagcaaa
      481 tcatgagatt agccagtcgt tttggttatg cgaaccagat acgtcgtgat cgtccgctga
      541 cacacgaaga actgatgcgc catgtaccga gtatttttgg ggaaaaccga catacctctc
      601 gcagtgaaca ctatgcgtac attcccacta tcaccgtcct ggaaaatctg cagcaggaag
      661 gctttcagcc gttctttgcc tgccagaccc gcgtgcgcga ccagagccgc cgggaataca
      721 caaaacacat gctgcgtctg cggcgggccg gacagataac cggtcagcat gtccctgaaa
      781 ttattctgct caactcccat gacggttcat ccagctacca gatgttaccc ggatattttc
      841 gtgccatttg taccaatgga ctggtctgcg gtcagtctct gggagaattg cgtgttccac
      901 accggggaaa tgtggtggac agagtcattg aaggggctta cgaggtggtg ggcgtttttg
      961 accggataga ggagaagcgt gatgccatgc agtcgctggt cctgccgcca ccggcacgcc
     1021 aggcgctggc acaggcggca ctgacttacc gttatggtga cgaacatcag cccgtcacca
     1081 ccgccgacat tctgacacca cgacgccggg aggattacgg taaggacctg tggagtgctt
     1141 atcagaccat ccaggagaat atgctgaaag gcgggatttc cggtcgcagt gcaaaaggaa
     1201 aacgtatcca tacccgggcc attcacagca ttgataccga cattaagctc aatcgcgcat
     1261 tgtgggtgat ggcagaaacg ctgctggaga gcatgcgctg atgccgtttc actgaaagtg
     1321 cagtcctgac tcacatctgt tcctgccccg gggcttcttc cattcattta ctttttataa
     1381 ggaataacct catgacaacc gtttcgcata attccaccac accttctgtt tccgtaacca
     1441 ctgcatcagg gaataaccca cctcagctgg ttgccactcc cgtccctgat gaacagcgca
     1501 tcagcttctg gccgcagcat tttggcctca ttccacagtg ggtgaccctg gagccccgtg
     1561 tcttcggctg gatggaccgt ctttgcgaag actactgcgg tggtatctgg aatctgtaca
     1621 ccctgaacaa cggcggggca tttatggcac ccgaaccgga tgacgatgat gacgaaacat
     1681 gggtactgtt caatgccatg aacggcaacc gcgctgaaat gagcccggaa gccgccggta
     1741 ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg tacggagtgt tatgccatga
     1801 cggtccacta ttaccggctg cgggattacg ccctgcagca tccggaatgc agcgccatta
     1861 tgcgcatcat tgactgaatg gaggcaggga caatgcagca gctttccttt ctgcccggag
     1921 agatgacgcc cggcgagcgc agtctcattc tgcgggccct gcaaaccctg gaccgccatc
     1981 ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc acgggaatgg ctgattctga
     2041 acatggcggg actggagcgg gaagagttcc gggtgctgta tctgaacaac cagaatcagc
     2101 tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg tacggaagtt catccccggg
     2161 aagtgattaa acgcgccctg taccacaatg ccgctgccgt ggtgctggcg cacaatcacc
     2221 cgtccggtga agtcacaccc agtaaggcag accggcttat caccgaacgt ctggtacagg
     2281 cactgggcct agtggatatc cgggtgccgg accatctgat agtcggtggc aaccaggttt
     2341 tctcctttgc cgaacatggt ctgctttaac ccgtcacaac cacatcacac ctgttttcac
     2401 ttttatcttc tgtcttcaga ggtatcacat catgagaatc atcacccgtg gtgaagccat
     2461 gcgtattcac caacagcatc ctgcatcccg tctttttccg ttctgtaccg gtaagtatcg
     2521 ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg caggatattc ccggcgttct
     2581 tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg tatgtccggc tgatgagtgt
     2641 caccctgaac tgatgttccg tcacccggga aacgagtacg accagatgac ctcagacgcc
     2701 gctgtggatg aggccattct catcaatgca tacatcttca ccgaaaccgg tcagcgcgcc
     2761 ggctgacctg tcatcgttaa accattctta accacttttc agagaggatt ttatcgtgtc
     2821 agacaaactc tccgggataa ctcatcccga tgacaatcac gaccgcccct ggtgggggct
     2881 tccctgcaca gtgaggccct gttttggcgc tcgtctggtg caagagggta accgcctgca
     2941 ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc gacgcagatg cataccatct
     3001 ggaccaggcc tttccgctgc tgatgaaaca actggaactc atgctcacca gcggtgaact
     3061 gaatccccga tatcagcata ccgtcacgct gaatgcaaaa gggctgacct gcgaagccga
     3121 caccctcggc tcctgtggtt acgtttatct ggctgtttat ccgacaccag cacccgcaac
     3181 cacctcataa ccccgaagct ctcaccttta gcactaccag ttacagagaa cttaagatga
     3241 aaacattatc cgacacacat gtacgggagg tatctcgctg cccgtctccc gtcaccatct
     3301 ggcagacact gctcatccga ctgctggacc agcattatgg cctcacactg aatgacacac
     3361 cgtttgccga tgaacgtgtg attgagcagc atattgaggc aggcatttca ctgtgtgatg
     3421 cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac cgaccagccg ggattcagca
     3481 cctgtccccg ctctcagtta ataaacagta ttgatatcct ccgggctcgc cgggcgaccg
     3541 gcctgatgac ccgcgacaat tacagaacgg taaataacat taccctgggt aagtatccgg
     3601 aggcgaaatg atgaaactgg ccctgacact ggaagccgac agcgttaacg tacaggcact
     3661 gaacatgggg cgcattgtcg ttgacgtcga tggtattgag ctcgctgaac tgattaacat
     3721 ggtctgcgat aacggctact cccttcgtgt tgttgatgaa tctgaccaga cctcagcaga
     3781 atgcacgcca ccatttgct
//
LOCUS       DAZFKY010000286         3792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFKY010000286 DAZFKY010000000
VERSION     DAZFKY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(77..346)
                     /locus_tag="ACHKCL_004641"
     CDS             complement(77..346)
                     /locus_tag="ACHKCL_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765454.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEGEMKQ"
     gene            complement(347..3253)
                     /locus_tag="ACHKCL_004642"
     CDS             complement(347..3253)
                     /locus_tag="ACHKCL_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO5765455.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNISGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEIVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKKQYLFNPPWESLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGDV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFELKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQANPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRT
                     RVKTGVTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRN
                     VEKIGNAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKP
                     GILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRY
                     GIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTG
                     KMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEK
                     QRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
     gene            complement(3364..>3792)
                     /locus_tag="ACHKCL_004643"
     CDS             complement(3364..>3792)
                     /locus_tag="ACHKCL_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765456.1"
                     /translation="AAPRTYHSFEPVNEPKNIMFEHVRTECEKTPDRHEETDKAFEEI
                     FWCAGMRKAGKKNAASAFRTQFREWRKTTRGTASEFATMLAEDIACRLGKQFGFDRLL
                     PSSYLNGQRWNDEKPETIQPQSKPSSAITVSKTGYVFFDR"
BASE COUNT          859 a         1053 c          823 g         1057 t
ORIGIN      
        1 cctccaatcc ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttacaagc
       61 tcagtgatgt agatggtcat tgcttcatct ccccttccat ttcatcaatg tcaacgtcat
      121 caggaagatg ggaacaatac gctgctatac catgatgatt tatctcatac cctttgaacg
      181 ttaccatctg gcgcgtaatc tcaacttcgt tcaggaatcc gccatcgcat aactgcctgg
      241 ctattttcga tttggtctgg attattggta gtgcctgttc tttcaaagcg tatgatattt
      301 gtgcatccca tgccttttcg agaatggcta attgtttttt attcatttaa tactccgtca
      361 cgttttcctg tcgccacgcc tcgtcatatt ccgatttcgg catattggcg atgtagctat
      421 atggcgatcc tgattcaagt tgcaggaact ggtgcgattg ctcgtcaagg aacaacggga
      481 caccaccttc ccaaccttcg ccgttacgtt gtttttcaag catcaaaaca gatgccggag
      541 atgccagtag ctgttcgtcc ttctctgaca tcttttcacc actctgaact ctctgtaacg
      601 ctctctcgcg agccttgtta cgccagatga tgaaaaggtt gtctgtcagg tctgttatcg
      661 ctccagagcc ttttacgtcc attttcccgg ttggtttttc ttcgctgtct ccttttcgcg
      721 agtgagtaac gagaatgacg tgggagtttg ttttgttttt gaagtcgcaa atcgagtcaa
      781 caaacgcctt ctgcccgtta tagtcatcgt cgcctatgcc acatttcatc aggctgtcga
      841 tgatgaataa ctggataccg tatcggcggc gagcgtagtc gaatatttcg atcagcctgt
      901 cggctttcgc cgttccggtc aggccaaaca cccaaagtct ttcgtcataa aatttaaatg
      961 cagagtcaat ttccagcact ggcggcatct tgcagcacgt cgcctgacgg gtaaggcgct
     1021 taaggagaat accaggcttc agctcaagtg acgcgatgca cgtcttcaca ccctgacgca
     1081 ttgcctcaag tgccatatgc ccgacaacct cggtgttatg cgtcgccaca tatcccctgg
     1141 ttacatacag gtggcgaggg tgatcgacca taatacacaa gcattctgca ttgccgattt
     1201 tttcaacatt cctgacgaat accccacagc cctcgaatcg atgcgcagtt aatctgccat
     1261 tgagtcgtgg tgaacggatg gcctccctga tttctcttgt cagtctgatt cttgcctcgt
     1321 atgaatccat cccatgccgc ttatcgtcct tgtatgtgta tgtcacgcca gttttaactc
     1381 gcgtccggca tgacccgccg agtgagttaa ccagttgaac aaccccattg cgcagttctt
     1441 cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc gtttcaagca
     1501 gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca cgagggatga
     1561 atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc atgagtggat
     1621 ttgcctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag aagttgtaat
     1681 caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa aacttcacgc
     1741 tcccattgct aagactacca tccccgagaa gggagccgat aacccatgct aatggctctg
     1801 agtggtcgcc aaagtcacca gttatttcag gaatcctaac gccatttttg tgcctcttcg
     1861 tttcactcaa ccgcttcagc tcgaaggtgt caatcacgcg gcgtttctcg cctttcgtga
     1921 atccacgact agtgacctcc cataggtgat cgcctgcgca atcaacataa cgaccatctt
     1981 caaatgtgac tcggtaaaca tctctaacac cctgcgggaa tatcccagtg acagttgacg
     2041 gattgccgtc tactgacgcc acctgatcgc caatttttac atccccgtga gtagtccatg
     2101 tcccatcagc taaaagaata ggctcattca gtgggcatgc ctttccgtga ccgttcacac
     2161 cattgaccag cgtcaactcg gcctcacgga actggaattt atctgccaga gattcccacg
     2221 gtggattaaa cagatactgc ttcttgccgt agaaagcgtt gatagtgtcc tggtaaaact
     2281 ctcgcgcact gtagagttct tcaggatcga aataggatgc cgtgccgatg tactgccaga
     2341 tttcatcctc ggtaacaccg ttcatcaggc attcgttgat gtctttgtac ggcagagtaa
     2401 caagacggca acgatgttca ccgagtcggc ttgcgatttc ccttgcggct tcacgaccaa
     2461 catcatcaac gtccatcgag atgaatattt cctcaaacct gtcgaggttg tgatactcaa
     2521 actcaatcca ctgctgctta gcgcctttcc cgccaccaaa cggcacggat aacgccgaga
     2581 tgccgtattg cgcatagctc atacaatcaa tttcgccttc gcaaagtaca accgccctca
     2641 cgccagcgtc cagagcctgc catccgaaca gacaaggttc gcaatcacct tctgccataa
     2701 tgactttctt cccgtccggg cgctcagtgc tgattcgctt gacctgcaac aactcaccat
     2761 cgcgtttgta cggaagcacc agagcatcca gttcccgctc tccattccac accttgccgc
     2821 tgacaacctc gtagcgcttt acgatttctg gcgatatgcc acgcgattgc aggtactcaa
     2881 gatgggattc tgttctggta acgtagcggg cgattttctt gcggtcaggt ctggagaatt
     2941 ttttctcacg tttggcatcg aaatggtgat cgtcatcctt gataccgaga aatgctttcg
     3001 cttcctgcat agcctgatgc aggttaattc cacgacatgc catccacaaa tcaagcatgt
     3061 caccgccgtc tccctcagcg aaatcagccc attttttctt gccgctaatg ttgaccttaa
     3121 ggctgtttcc cttgtcaccg ttgacgttac cggcaaccca ctcatgcccc tctttcttgc
     3181 cgtttggcaa caggtgcgga gccaccctgt caacctgcgc ccaaagcagg tcgctcagtt
     3241 cacttggagt cattacgctg acctcagatc gagacggtta aaccagaact caacgaatgc
     3301 agaactaagc cagccatggt tatagccagc gataagtaac gatttgattc tggatttcat
     3361 ggttcacctg tcgaaaaaca cgtagccagt tttcgatacg gtgattgcgg atgatggttt
     3421 ggattgtggt tgaatagttt ctggcttctc gtcgttccag cgttgaccgt tcaggtagct
     3481 cgatggtaac aacctgtcga acccgaactg cttaccgagc ctgcacgcaa tgtcttctgc
     3541 cagcatcgtg gcaaactcgc ttgccgtccc cctggtagtt ttacgccatt ccctgaactg
     3601 tgttctgaat gctgaagctg cgtttttctt cccggctttc cgcatgcctg cacaccagaa
     3661 tatttcctcg aatgccttgt cggtttcttc gtgacggtca ggagtttttt cacactctgt
     3721 tcggacatgt tcgaacataa tgtttttagg ttcattgact ggttcaaaag agtgataggt
     3781 tctgggggca gc
//
LOCUS       DAZFKY010000287         3714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFKY010000287 DAZFKY010000000
VERSION     DAZFKY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(231..1502)
                     /gene="efeB"
                     /locus_tag="ACHKCL_004644"
     CDS             complement(231..1502)
                     /gene="efeB"
                     /locus_tag="ACHKCL_004644"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5765457.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(1508..2635)
                     /gene="efeO"
                     /locus_tag="ACHKCL_004645"
     CDS             complement(1508..2635)
                     /gene="efeO"
                     /locus_tag="ACHKCL_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5765458.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2693..3523)
                     /gene="efeU"
                     /locus_tag="ACHKCL_004646"
     CDS             complement(2693..3523)
                     /gene="efeU"
                     /locus_tag="ACHKCL_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5765459.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(3535..>3714)
                     /locus_tag="ACHKCL_004647"
     CDS             complement(3535..>3714)
                     /locus_tag="ACHKCL_004647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765460.1"
                     /translation="GETSAKYLKIIAFFSGFDLLIFTFQHHFINIPLVFFFSVMIIAI
                     TAIYFSLHRLTQKNV"
BASE COUNT          913 a         1036 c          879 g          886 t
ORIGIN      
        1 aacaccattt cagtgacatt agtttctact ggaaagatga cagagtgatg acagatatga
       61 aaaaagctgt gtgctttcag caggatttgc atcaggcatc agtgcacgat cgccgtatgc
      121 ggcgtgaacg ccttatccgc ctaccgaacc cgcaccgatt gtaggcataa gaagacgcgc
      181 aagcatcgca tcaggcatct gcgcaagaac gccttatccg tctaaaaaca ttaaacccgc
      241 aataacgcgc ttccgagata atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa
      301 ccgccgccga taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact
      361 gtcaggaagc ctttttccag atcgtgttgg taacagacaa acagtagccc catatccagt
      421 tgcccggagt tggtgacgcc cagtgaataa ctgtagccac gacgcagcat caggctggac
      481 tcactctccg ccgtgcgggg attcgccagc cggatatggc tgtccagcgc aatccccttc
      541 ccttccgggt cgctggcgta atcaggcaca tcatgctcat gctgcattcc cagcggcgca
      601 ccggtttgct tatcacggcc aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa
      661 aattccactc gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca
      721 ggctcctgct gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta
      781 tcgggattgg cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta
      841 ctacgcgccg cgtgatcgga aataaaccct tcccgcttcc agcgcacgct gagcaaatcc
      901 ggcgtatgtt tgatgatatc gcgcagtgca tggatcaccg tgtcctgggt gttggcgcaa
      961 atttgtagca acacatcacc atgacataac gccgcatcca gcgagtcgtt gggaaaacgc
     1021 gtcatcttct gcaacttttt cggcatctgt ggcgcaaggc caaagcgctc atcaaacaat
     1081 gagtgaccca ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg
     1141 ccggaatcga gtggtggcag gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc
     1201 agaaaagcaa aacgctgagt caacaagcga aacaaccgtt caagatcggc tttatcgctg
     1261 gcaagcacat caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc
     1321 tgatgctcac cataaaacgg ctgtttctca ttgcgtgcaa ccggtgaaag cgtacccggc
     1381 gcactttgcg ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacca
     1441 atgcctttca gtaaacgtcg gcgtgacggt tcattcacgc cgtttttatc ttcatactgc
     1501 ataatgctta atccaatccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag
     1561 taatcggtcc tttcagtgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac
     1621 cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt
     1681 ttgccaacag ttccgggtta gctttttgca gttgtggacg cagcagatcg acaattttct
     1741 gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt
     1801 caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcaccg ccgaccactt
     1861 ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa
     1921 gctggtcagc gtactgatcc atgcctttgg tggtgttgtc gccaaacaat gctttttcca
     1981 gacggtggaa accggtgaat tttggatcag cggctttttg ctcgtaatca tcttcacggg
     2041 cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt
     2101 gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt
     2161 cggtaaaggc tttggtgtcg gtcaccagct gtgtggtttc cgccatgaca tacgctttat
     2221 atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg
     2281 cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt
     2341 attcgccagg ctgtaaattc gccgtcattt tctggctaaa gccaggggcg atattttccc
     2401 gctcttccac caccatcacg cctttgagga tctcccattc cagcgccttc tggctgtggt
     2461 tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct
     2521 tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag
     2581 aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga
     2641 gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg
     2701 agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga
     2761 gataaataaa ccagacggcg acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc
     2821 cttccatcag cgtgccaaac agcgagtgag ttgagagcac cgcactcata tcgaaggcga
     2881 tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca ccagctgcca
     2941 gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga
     3001 ggcgaatacc gccccagtag agcaggaagc ctagcaccac ggcagtagca agaccgagca
     3061 ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga
     3121 ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat
     3181 gatgatttcc acgctgcaat gcgctatcga cagcctgctc cagttgtact ttgacgttgc
     3241 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     3301 cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga
     3361 agatccccaa gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacaccaa
     3421 tccatcggcc tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg
     3481 cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac
     3541 gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac
     3601 gctaaagaaa aatacaagcg gaatatttat aaaatgatgt tggaatgtaa aaatgaggag
     3661 atcaaatccg gagaaaaaag cgattatttt aaggtattta gctgacgttt cgcc
//
LOCUS       DAZFKY010000288         3700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFKY010000288 DAZFKY010000000
VERSION     DAZFKY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(66..482)
                     /locus_tag="ACHKCL_004648"
                     /pseudo
     CDS             complement(66..482)
                     /locus_tag="ACHKCL_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(482..667)
                     /locus_tag="ACHKCL_004649"
     CDS             complement(482..667)
                     /locus_tag="ACHKCL_004649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765461.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            844..3054
                     /gene="katP"
                     /locus_tag="ACHKCL_004650"
     CDS             844..3054
                     /gene="katP"
                     /locus_tag="ACHKCL_004650"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO5765462.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3098..3487
                     /locus_tag="ACHKCL_004651"
     CDS             3098..3487
                     /locus_tag="ACHKCL_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5765463.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3544..3700
                     /locus_tag="ACHKCL_004652"
                     /pseudo
     CDS             3544..3700
                     /locus_tag="ACHKCL_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3600..>3700
                     /locus_tag="ACHKCL_004652"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1045 a          781 c          952 g          922 t
ORIGIN      
        1 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
       61 agtcgcacat ctgcctggcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
      121 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
      181 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
      241 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
      301 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
      361 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
      421 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
      481 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      541 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
      601 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
      661 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
      721 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
      781 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
      841 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
      901 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
      961 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1021 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1081 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     1141 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     1201 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     1261 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     1321 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     1381 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     1441 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     1501 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     1561 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     1621 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     1681 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     1741 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     1801 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     1861 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     1921 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     1981 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2041 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     2101 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     2161 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     2221 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     2281 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     2341 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     2401 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     2461 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     2521 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     2581 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     2641 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     2701 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     2761 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     2821 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     2881 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     2941 gcggtggcag aagtatatgc ctcggatgat gcgagaaata agtttattca tgattttgtt
     3001 aaatcgtgga ataaagttat gaatagcgat cggtttgatt taaacaataa atgataaaac
     3061 tgatgaggga gggcacctcc ctcatgaagg agaaattatg cagaaaagca ataaagttat
     3121 aatcagctgt ggtatggcat tatatttttt gtgtgggggg attacagtgg cagaagaact
     3181 tgatgaaagc atgcaagttc ttgcaaggaa tatgagtgta cttcagtcat caatggataa
     3241 aaaggagctt cttgaagctc ttgacagaat ggatgatgct gtagatgagt caatgaagcg
     3301 tcttcctgag agcatttcac cggctgatga agaagggaag aatgactata taaaagaact
     3361 tcaaaagctt gaaaaagaga ttatgctggc gaaacaaaaa gtcttatcgg gacagttatc
     3421 agatataccc gattcggtaa acaagatgaa taaaatcaga gttgaggggc atgataaatt
     3481 cagataaatc agttggttga ttcctgcggg acgaatacat acgtcaggaa cataacttta
     3541 tggctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     3601 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata
     3661 agctgcgtga gcagtacggg gtcggaccgg tatgcagtga
//
LOCUS       DAZFKY010000289         3650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFKY010000289 DAZFKY010000000
VERSION     DAZFKY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1001)
                     /locus_tag="ACHKCL_004653"
     CDS             complement(<1..1001)
                     /locus_tag="ACHKCL_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO5765464.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVISTLSKFFNVSGLGAISVIKFIVMALFA
                     "
     gene            complement(1011..1691)
                     /locus_tag="ACHKCL_004654"
     CDS             complement(1011..1691)
                     /locus_tag="ACHKCL_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO5765465.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1678..2145)
                     /locus_tag="ACHKCL_004655"
     CDS             complement(1678..2145)
                     /locus_tag="ACHKCL_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO5765466.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2145..2681)
                     /locus_tag="ACHKCL_004656"
                     /pseudo
     CDS             complement(2145..2681)
                     /locus_tag="ACHKCL_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2745..3392
                     /locus_tag="ACHKCL_004657"
     CDS             2745..3392
                     /locus_tag="ACHKCL_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5765467.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1026 a          868 c          840 g          916 t
ORIGIN      
        1 gcaaacagag ccattactat aaatttaatt acacttatag caccaagacc agaaacatta
       61 aagaacttgc tcaatgtact gattactata gtatggacca agtaaaatga aaaagaagca
      121 tcacctaaaa gtatcataac tttatttgat attatttttg aaataatacc accattaaac
      181 gaaaacacta atattgaaaa ggccattgat ggtataaaaa gcgcatcata ctgtaatcca
      241 ttgctatttt gactttttgc caccatatag acagacatta ggataaagac tacagatgat
      301 atttcagcaa atgatagaac taaatatgac ggctttgact taaactcaaa atctccggcc
      361 tgattgctgc ggattgtctc tgccagcctg cctcggtcaa tctcacgacc agccatcttg
      421 tcctgaacat tgaagtagtc aatcggaccg agagcagcca tcccaaggtg ataaacaaac
      481 tcaccaaacc ctgaaggatt ctgctgatac atctgagcaa cgttgttagg gtcaacaccg
      541 acgcgcgcca gttccttggc gttgttttgc agccatgatt gcattgcttc tggagacgag
      601 gccgcaaggc gtgcgccagc cgctaaggtg ccgatagaat tgcgctggtc ttcgtctgcc
      661 cacttcatac cagactgaat cttctctaat tgaccaggat atttggtcat cagatctcga
      721 acctgctgtc gatcgccgga ctggatggct gccgcatatt ctttttggaa tgcagcatcc
      781 gcttcctgtt gctttgcggc ttgagatgtt tgagcgacac taccaagtcc ctgcaacgcc
      841 tgaaggccga tgttattgcg acctgaacgc tccatttcgt tgttctggcg aatataggcc
      901 aacgcctcac ttacatcact tgcctttggc gcatttgagt tttgcccacc gataccagca
      961 agaaaaccgc ctgagttgat tccttgttgc caagtagcca tattcccacc ttaaaacaat
     1021 gatccaagac caccgataac accgccagca agagcaccaa taccagtccc aataacagga
     1081 actgcactac caatcattgc acctgatgcg gccccaccaa tggcacctgt agcaaaactc
     1141 tgtaggcctg aaggcttatt cgcatttgct gccgatgctg ccgcctgctg ttgatacaat
     1201 tggctgacat tgttagcgta gttctgtccg gcgtttgcct gacctgtaag agcaccaagg
     1261 ccgatatttg ccagattgtt gtagttgttc atctgacctg acagccagtt ttgaccgagt
     1321 gtaggtgcga ttgctgctaa ctggtttcct gttgctgtag agcctaatcc acccgttgcc
     1381 tctgctgctg ccagactctg atagcgcgcc tgccctgcaa ggtctttata ctgctgagag
     1441 ttgtaatact ggttaagcgc ctgaccttgc ccctgaagag aggaaagatt ctgcaactgt
     1501 gatacgtact gctgagcgag tggcgtgaac ggtgcaaggt tttgcatgtt cgtctgccac
     1561 atttcacgct gcagttcgat gcccttttca gttgcgcgtg cctgggcttt agatccggag
     1621 tcgctgccgc ctttcatata cccattcatg ggaagcaatt tattcttgaa gctttcgcta
     1681 agtactaaca tttaatagct cctcatattt cgaacgaggt aattgataga gggtgattcc
     1741 taccggtttt ccgttactca tgtacgcatc atcaaggtga ccaacacggg tagcgccaag
     1801 caaacggata attgcccgtc cgtatttggt ggtgtcagga accatggtga tgctgttaag
     1861 gaatggtgag ttttcgagaa gccatttgca gaataatcga tgcccttgta gtgcatattc
     1921 gccacggaat ccggggtcat acaccgcatg gcattcaaca acgctatgcc agaagttacg
     1981 cacttcatga actccagcca gcactaatcc ttcgtagatg ccgagatata ccgcatcagg
     2041 cttgatgtag tatttatctc cactgtctac gatattcccc gtgtttaccg ggttgttaag
     2101 gaattctgca agtttcaccg ggttatcgat gagctttatt tccatcactg ctccgcaatg
     2161 attttgatgg ttgtggcagt aaacgccgcg ccattcgact gaatggttaa cgtgctgcca
     2221 tttgtggcaa gaaagccgtc tttatccacg ctgaagaacg tagctaacag gatgttgtcg
     2281 gttgttgttg ccgagttgcg actgcttact agcgtgtcag gaacagaacc ggagaatgtt
     2341 agctgcattg acctgttggc ggttccgctg ggccacgtcc cgacaatcga cagcttgaag
     2401 aacaaggttt tgttctcgtt gaacacaacc atcttgttgt taacggtgtc gaagaatggt
     2461 gccaacgtcc cggatgacgg cgtaagcgtt ttcagcaggc taacaaggtt ggtcggcgct
     2521 gtcgggatgg ttactgatac gccagagtaa acaacctctg acttcttgcg cgtagtggca
     2581 tactccagag cattaatgcg cgtttcatgg tctgaaacct gcgattccag cgactgaact
     2641 ctggtatcaa gcgatgcaat atcgctttca ttcaaggcaa tcagtgggtt tacgtcatgg
     2701 agtgggctca acaccagtca gagccgcatg tacacgcaat aatcatggtg aacgaaagaa
     2761 gcattaatgg gcaagctggc aaaattgtca gcaaatgggt aacactggca aacaatgcaa
     2821 tcccatcaaa tgttcctccc cagtgggatc tagatttgaa aggtcagcaa tattcagagc
     2881 ctacaaagtg tataggtaaa cagattgatt acatggcaaa gcccgaaact aagttaaatg
     2941 actttaatag agaggcgagc aagagcctat atgattggtc agatgccaac gtttggggtt
     3001 attcgaatgg ctgggcgcgg catgagataa aaaaagaaac actttctatc actggctttc
     3061 atgctgtaag acgcatacta aaagccgttc aggcatcaag gaaagacaac tacaaagcta
     3121 agcgacagat acagcgtaca ttgaaacgac aagacataaa tcgatcacca atcaccccgt
     3181 tccaagcacc gttaattagc aaacacgatt gggatttatc agtgatagcg gtcaccaaag
     3241 aggcagagac agagcgcagg gaattactat cctcacagat aataaactcg aacaaagtac
     3301 aaacccaccc actgttaagg atgttaagaa gagcacgagt gagagcatcc agagccaaaa
     3361 gagaaagcgg aggttatgac cattactact agtagtgaca gatcgtgaca aggcggtgca
     3421 aaaaacgacc aataataaga gagatatgcc tgccaaaccg ccaaaagcgc ggagcgcggc
     3481 aaacgccgcg agaggcgcaa gaaggcgaac ctatccattg aaacggtcag aagcgcatcg
     3541 cgctcgacga ccgaagtccc cactaacacc gttaacgaaa gatcaaagtc ttagaaaaaa
     3601 atgacgtttc tttgatggac ttccatccgt aaaaatcaaa ttttgagaaa
//
LOCUS       DAZFKY010000290         3633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFKY010000290 DAZFKY010000000
VERSION     DAZFKY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(137..1294)
                     /gene="intS"
                     /locus_tag="ACHKCL_004658"
     CDS             complement(137..1294)
                     /gene="intS"
                     /locus_tag="ACHKCL_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO5765468.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1456..1530)
                     /locus_tag="ACHKCL_004659"
     tRNA            complement(1456..1530)
                     /locus_tag="ACHKCL_004659"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1495..1497),aa:Arg,seq:cct)
     gene            complement(1606..2538)
                     /gene="yfdC"
                     /locus_tag="ACHKCL_004660"
     CDS             complement(1606..2538)
                     /gene="yfdC"
                     /locus_tag="ACHKCL_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5765469.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2832..3587
                     /gene="mlaA"
                     /locus_tag="ACHKCL_004661"
     CDS             2832..3587
                     /gene="mlaA"
                     /locus_tag="ACHKCL_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5765470.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          862 a          883 c          862 g         1026 t
ORIGIN      
        1 gaaatgcata tgtagttaac atataataat tgcattttgt tattgttgat ggtttcttgg
       61 tgtaagaaat cctgagaagt gaaaagcttt gctatatgtg caaggctttt cttttttata
      121 agaatcgttg taataactac tccaccttcc catcaagcca gtccgcccac cactgcatca
      181 tttctctgcg cttatcgaga tactgagcat ggttgtaaat cccacgcaca gatccgccgt
      241 tggcatgtgc tagttgcact tcaatagcgt cagcaggcca ttcgtgctcg ttcataatcg
      301 tgctgaattc atgcctgaat ccgtgaccgc tttccagacc ttcatagccg atttgtttga
      361 tcacaagcag tacagcgttc tcacagattg acttcttctt atcgttgcgc ccggcaaaaa
      421 caaactctga tactggttta gtgatggagc ttagcgtagt gagaagttca acaacctggt
      481 ccgacatcgg aaccacatga atcttgcgtc ccttcatcac actggcctcg atggtgataa
      541 tcctgttttc aaaatcgacg ttcttccatt gcatggaacg aagctctttc gttcttagtg
      601 ctgtgtagcg taaaactttg gtcgcaatga gagatacgat acttcctgaa aatgttgcaa
      661 gtgctttgtt gaatgccggg atctggtcgg caggtaaaaa cgggaagttc ttcttgcggt
      721 atcccttcat ggcttcagca aggtcaggtg ccgggttata tttagccctt ccggtgacaa
      781 tagcgtaacg gaaaacctcg ccgcatcttc tgcgtgcttt gttggctcgc tccattgccc
      841 cgcgatcttc aaatctgcgg attacttcca gcagttgcat cggctcaata tcctgaattt
      901 caaggccgcc gatgataggt aaaatgtcgt catcaaacat ttttgcaagt tcagtcgcat
      961 aggcggctga ccagacttgc ctcttatgct cgtaccattc cttgtaaatc gcactaaagg
     1021 aattgttgtt agacgaagcc tttttcgcct ttaccggatc gatgccaacc gagatgtctt
     1081 tcctcgcagt ccatgcttta tcccttgcct cttgcaaagt cataagcgga tattttccga
     1141 cggtcaggat tttctcctta ccgtcaatct tgtagcgaag ctgccatacc tttttccctg
     1201 atacagggac ataaaggtac aggccattac catcgagtag gcggtatggt ttttctttcg
     1261 gctttgctgc ttcaatctgc ttaacggtga gcatgggtaa aaatccggtg ggtaaaatta
     1321 ttttatccac tttttacccg tcatggcgtg cggctgtcaa cgatctgacg cgaaccatga
     1381 cgaactgtga atctacggaa ggcttgatat tcaggggatt ttgcggactg gtacggatgg
     1441 aggcgaactg ataaatggtg tcccctgcag gaatcgaacc tgcaattagc ccttaggagg
     1501 ggctcgttat atccatttaa ctaagaggac aatgcggcat gagtataccc gctaatggac
     1561 tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc gccatttatg ccgggttttt
     1621 atcatttttc ttaatgtttt ccgcacgttc tgctttttgg cgtgcttctg ctttgcgctt
     1681 gttgctcatg tcgttacgga tctgtgcatg actcattaac gcgaagataa aggtgccgcc
     1741 gcagatgttc cccgctaaag taggtagtgc gaagggccag atgaaatcgc tccagtgcag
     1801 cgtaccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt gagtggtgtc
     1861 acccagggca ataagccagg tcatcaatat aatcaccaca atctttgccg cacccgctgc
     1921 aggaaacatc cataccatag tggcgatcag ccagccggaa atgatcgcgt tggcaaacat
     1981 ctcgctgggg gtgttcttca tcacatccat gccgattttg acaaatgcat cgcgagtttc
     2041 ttcattgaag attggcatat attcaaatgc ccacgccgca atacctgtcc cgagaatatt
     2101 acccagcagc acgacgcccc ataaccgcat aagtaagcca acgttgctca ttgtcggttt
     2161 ttgcatgacg ggtagtaccg cagtcacggt gttttcggta aataattgct ggcgggccat
     2221 aatgacgata ataaaaccaa aggtataacc gagattctcc agcaagaagc tgcccggcac
     2281 accttccagt tcgacatgaa atatcccttt tgccagtaat gaagcgccca tcgacagacc
     2341 cgccgcaatg gctgaccaca gtagtgccat tgcgtcgcgt tccagctctt tttcaccatc
     2401 ctggcggata tgctcatgaa ttgccatcgc ccgggagggg agtcgatctt catctatttc
     2461 tatttttttg ccacgctctt tttcttcgct ctcaacttca atttcgtcgc tgtgttgatc
     2521 aattttgtcg ttgtccatgg tctcttctct tgaattagca cgtatagcta aagcgtagcg
     2581 gcttttttgc tcgcaactgg cgggagttac tttgaaaatg tagaaaaggc cgcgtttgcc
     2641 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
     2701 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
     2761 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
     2821 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
     2881 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
     2941 gcaccatgta caactttaac ttcaatgtat tagacccgta tattgttcga ccggtcgctg
     3001 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaacgg tttgagcaat tttaccggca
     3061 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
     3121 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
     3181 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
     3241 ttggtcatta tggcgtgggt tatggcccgt acgttcaatt accgttctac ggtagcttca
     3301 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
     3361 cctggccgat gtctgtgggt aaatggacgc ttgaaggtat cgaaacccgc gcacagctgc
     3421 tggattccga cggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
     3481 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
     3541 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     3601 aaggtgagtc gcaaaactca ccttttttgt tgt
//
LOCUS       DAZFKY010000291         3566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFKY010000291 DAZFKY010000000
VERSION     DAZFKY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..194
                     /locus_tag="ACHKCL_004662"
     CDS             <1..194
                     /locus_tag="ACHKCL_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO5765471.1"
                     /translation="KITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCF
                     RSNTLGAKMQALRIFLSRK"
     gene            469..1665
                     /gene="kbl"
                     /locus_tag="ACHKCL_004663"
     CDS             469..1665
                     /gene="kbl"
                     /locus_tag="ACHKCL_004663"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5765472.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYLVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1675..2700
                     /gene="tdh"
                     /locus_tag="ACHKCL_004664"
     CDS             1675..2700
                     /gene="tdh"
                     /locus_tag="ACHKCL_004664"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5765473.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            2939..>3566
                     /gene="waaH"
                     /locus_tag="ACHKCL_004665"
     CDS             2939..>3566
                     /gene="waaH"
                     /locus_tag="ACHKCL_004665"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5765474.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTE"
BASE COUNT          855 a          807 c          957 g          947 t
ORIGIN      
        1 aaaaaataac actaaataat aacattgtgg atgatgacca gggcattttt gtcatgtgct
       61 attacaaacg ccccgatctc ttcaacctaa actatctggg gagaggaaaa tggttcgatt
      121 tgtttcgctg cttcaggagt aacactttag gggcaaaaat gcaggcgctg agaattttcc
      181 tttcaagaaa ataggtttcc ctccattagt gggtagcgtt ttgataccaa ctgagatagg
      241 aaaaatgatg ctttggcatc attttttctt tttatatcaa tgcagtaaaa tcaatcatca
      301 ttatgcccag ccaaaactgt acagtttttc tactatctgt aggtgttgag agacatcaag
      361 ttacacgtca tttatcctga attttgcaga agagttaacg cgtcatctcg tcgcgaccta
      421 tacgtttggg taatatgtgc tggaatttgc cctgtctgga gaatcgcaat gcgtggagaa
      481 ttttatcagc agttaaccaa cgatctggaa accgcacggg cggaagggtt gtttaaagaa
      541 gagcgcatta ttacgtctgc gcagcaagca gatatcactg tggctgatgg aagccacgtc
      601 attaactttt gtgccaacaa ctatctcggg ctggcgaatc atccggatct gattgcggca
      661 gcaaaggcgg gaatggattc tcacggtttc ggcatggctt cggtgcgttt tatttgcggc
      721 actcaggaca gccataaaga gcttgaacaa aaactggcgg cattcctggg gatggaagat
      781 gcgattctct actcttcctg ctttgatgct aacggtggac tgtttgaaac gctgctgggt
      841 gcggaagacg ccattatctc cgacgcactg aaccacgcgt ctattattga tggtgtgcgt
      901 ctgtgcaaag ctaaacgcta tcgctatgcc aacaacgata tgcaggagct ggaagcacgt
      961 ctgaaagaag cgcgtgaagc cggtgcgcgt catgtgctga ttgccaccga tggtgtgttc
     1021 tcaatggacg gcgtgattgc caacctgaaa ggcgtttgcg atctggcaga taaatacgat
     1081 gccctggtga tggtggatga ctcccacgcg gtcggttttg tcggtgaaaa tggtcgtggt
     1141 tcccatgaat actgcgatgt gatgggccgg gtcgatatta tcaccggtac gcttggtaaa
     1201 gcgctgggcg gggcttctgg tggttatacc gcggcgcgca aagaggtggt tgagtggctg
     1261 cgccagcgtt ctcgtccgta cctgttctcc aactcgctgg caccggccat tgttgccgcg
     1321 tccatcaaag tactggagat ggtcgaagcg ggcagcgaac tgcgtgaccg tctgtgggcg
     1381 aacgcgcgcc agttccgtga gcaaatgtcg gcggcgggct ttaccctggc gggagccgat
     1441 catgccatta ttccggtcat gcttggcgat gcagtagtgg cgcagaaatt tgcccgtgag
     1501 ctgcaaaaag agggcattta cgtcaccggt ttcttctatc tggtcgttcc gaaaggtcag
     1561 gcgcgtattc gtacccagat gtctgcggcg catacccctg agcaaattac gcgtgcagta
     1621 gaagcattca cgcgtattgg taaacaactg ggcgttatcg cctgaggatg tgagatgaaa
     1681 gcgttatcca aactgaaagc ggaagagggc atctggatga ccgacgttcc tgtaccggaa
     1741 ctcgggcata acgatctgct gattaaaatc cgtaaaacag ccatctgcgg gactgacgtt
     1801 cacatctata actgggatga gtggtcgcaa aaaaccatcc cggtgccgat ggtcgtaggc
     1861 catgaatatg tcggtgaagt ggtaggtatt ggtcaggaag tgaaaggctt caaaatcggc
     1921 gatcgcgttt ctggcgaagg ccatatcacc tgcggtcact gtcgcaactg tcgtggcggt
     1981 cgtacccatc tgtgccgcaa caccattggt gtcggtgtta accgtccggg ctgcttcgcc
     2041 gaatatctgg tgattccggc gttcaacgct ttcaaaatcc ccgacaatat ctccgacgac
     2101 ctggcttcca tttttgatcc cttcggtaac gccgtgcata ccgcgctgtc gttcgatctg
     2161 gtgggcgaag atgtgctggt ttctggtgca ggtccgatag gtattatggc tgcggcggtg
     2221 gcgaaacacg ttggtgcacg caatgtggtg atcaccgatg ttaacgaata ccgccttgag
     2281 ctggcgcgca aaatgggtat cacccgtgcg gttaacgtcg cgaaagaaaa ccttaatgat
     2341 gtgatggctg aactgggcat gaccgaaggc tttgatgtcg gtctggaaat gtccggcgcg
     2401 ccgccagcgt ttcgtaccat gctcgatacc atgaaccacg gcggtcgtat tgcgatgctg
     2461 ggtattccac cgtctgacat gtctatcgac tggaccaaag tgatctttaa aggcttgttc
     2521 attaaaggca tttacggtcg tgagatgttt gaaacctggt acaagatggc ggcgctgatt
     2581 cagtctggcc tcgatctctc gccgatcatt acccatcgtt tctctatcga tgatttccag
     2641 aagggctttg acgctatgtg ttcgggccag tccgggaaag ttattctgag ctgggattaa
     2701 cacgaaaaag ggctggtatt ccagcccttt tgcctgagga taatctgtta aatatgtaaa
     2761 atcctgtcag tgtaataaag agttcgtaat tgcgctgatc ttttatatag ctgttctcat
     2821 tatctctcta ccctgaagtg actctctcac ctgtaaaaat aatatctcac aggcttaata
     2881 gtttcttaat acaaagcctg taaaacgtca ggataacttc agaggtcgtc ggtaatttat
     2941 gatgaacagc accaataaac ttagtgttat tattccgtta tataatgcgg gcgatgattt
     3001 ccgcacttgt atggaatctt taattacgca aacctggact gctctggaaa tcattattat
     3061 taacgatggt tcaacggata attctgttga aatagcaaag cattacgcag aaaactatcc
     3121 gcacgttcgt ttgttgcatc aggcgaatgc tggcgcatcg gtagcgcgta atcgtgggat
     3181 cgaggtggcg acgggcaaat atgtcgcttt tgtcgatgct gacgatgagg tctatcccac
     3241 catgtacgaa acgctgatga ccatggcgtt agaggacgac ctcgacgtgg cgcagtgcaa
     3301 cgctgactgg tcttttcgtg aaacgggaga aacctggcaa tccatcccca gcgatcgcct
     3361 tcgctcaacc ggtgtattaa ccggcccgga ctggctgcgg atggggcttt cttcgcgccg
     3421 ttggactcac gttgtctgga tgggggttta tcgccgtgat gttattgtta aaaataacat
     3481 taaatttatt gccggattac atcatcagga tattgtctgg acaacagaat tcatgtttaa
     3541 cgcgctgcgt gcgcgatata ccgagc
//
LOCUS       DAZFKY010000292         3566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFKY010000292 DAZFKY010000000
VERSION     DAZFKY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            51..629
                     /gene="espA"
                     /locus_tag="ACHKCL_004666"
     CDS             51..629
                     /gene="espA"
                     /locus_tag="ACHKCL_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO5765475.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            642..1766
                     /gene="espD"
                     /locus_tag="ACHKCL_004667"
     CDS             642..1766
                     /gene="espD"
                     /locus_tag="ACHKCL_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO5765476.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1787..2725
                     /gene="espB"
                     /locus_tag="ACHKCL_004668"
     CDS             1787..2725
                     /gene="espB"
                     /locus_tag="ACHKCL_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO5765477.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2732..3139
                     /gene="cesD2"
                     /locus_tag="ACHKCL_004669"
     CDS             2732..3139
                     /gene="cesD2"
                     /locus_tag="ACHKCL_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO5765478.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3175..3396
                     /gene="escF"
                     /locus_tag="ACHKCL_004670"
     CDS             3175..3396
                     /gene="escF"
                     /locus_tag="ACHKCL_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO5765479.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3402..>3566
                     /gene="escG"
                     /locus_tag="ACHKCL_004671"
     CDS             3402..>3566
                     /gene="escG"
                     /locus_tag="ACHKCL_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO5765480.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGL"
BASE COUNT         1053 a          676 c          859 g          978 t
ORIGIN      
        1 actcaataat ttttttgttt tcctgagaaa aattatcaag aggtatatag atggatacat
       61 caaatgcaac atccgttgtt aatgtgagtg cgagttcttc gacatcgacg atctatgact
      121 taggtaatat gtcgaaggat gaggtggtta agctatttga ggaactcggt gtttttcagg
      181 ctgcgattct catgttttct tatatgtatc aggcacaaag taatctgtcg attgcaaagt
      241 ttgctgatat gaatgaggca tctaaagcgt caaccacggc acaaaagatg gctaatcttg
      301 tggatgccaa aattgctgat gttcagagta gcactgataa gaatgcgaaa gccaaacttc
      361 ctcaagacgt gattgactat ataaacgatc cacgtaatga cataagtgta actggtattc
      421 gtgatcttag tggtgattta agcgctggtg atctgcaaac agtgaaggcg gctatttcag
      481 ctaaagcgaa taacctgaca acggtagtga ataatagcca gctcgaaatt cagcaaatgt
      541 cgaatacatt aaatctctta acgagtgcac gttctgatgt gcaatctcta caatatagaa
      601 ctatttcagc aatatccctt ggtaaataac cggagataac tatgcttaac gtaaataacg
      661 ataccctgtc tgtaacgtct ggggttaata ccgcctcggg tacttctggt attactcaat
      721 ctgaaacggg tttatcgctg gatttacaac tggttaaatc catgaactcg tcagcaggct
      781 ggacagaaag tagcccttta ccgacgccgc cggcaggtca ctcattagtg acgccctctg
      841 ctgctgagga tgtccttagt aaattgtttg gtggtattag tggtgaggtt acaagtcgca
      901 ctgaggaggc agagccacag cgcacaagct atccctatct ctctcaggtg aataccgttg
      961 accctcagca aatgatgatg atggtcactc tgttatccct ggatacttcc gcgcagaaag
     1021 tctcgagtct gaaaaactct aacgagattt atatggatgg gcaaactaaa gcgctggaga
     1081 ataaaacgca ggagtataaa aaacagctcg aagaacaaca gaaagccgaa gagaaatcac
     1141 aaaaaagtaa aattgttggc caggtctttg gttggttggg cgtcgcatta acagccgttg
     1201 ccgctgtttt taacccagca ctctgggctg ttgttgccat tggtgcaaca gcaatggcac
     1261 tgcaaacggc agtcgatgta atgggggaaa atgcccctca gggattaaag actgcagcac
     1321 aggtctttgg cggaatatct atggccgcaa gcattctgac agccggcgtt ggcggggtgt
     1381 cttcactgtt atctaaattt ggtaatgttg ctaacaaaat tggctcaagc gttgtaaaag
     1441 tcgttgagaa ggcggcagaa gcgctggtta aaaacgtttt tgcaaaaatt tcgacagtgg
     1501 ctgagggcgt tacgaacggt attcgttctg ccgggacaac tgcgttgaat aatgaggctg
     1561 cgcaactcca aatgttgtct cagttagctg ctttcgcggt gcaaaactta actcgacaga
     1621 gtgaaagctt aggtgagagt gcgaagctcg agctggataa agcggcaagc gagttacaaa
     1681 atcaggcgag ctatttacaa agtgtttctc aactgatgtc cgattcagca cgggtaaata
     1741 gtcgtattgt tagtggccga atttaattat taaagagaat tttattatga atactattga
     1801 taatactcaa gtaacgatgg ttaattccgc ttcggagagt acgaccggcg cttccagtgc
     1861 agttgccgca tctgctttat caattgattc atctctgctt actgatggta aggttgatat
     1921 ttgtaagctg atgctggaaa ttcaaaaact cctcggcaag atggtgactc tattgcagga
     1981 ttaccaacaa aaacaattgg cgcaaagcta tcagattcag caggccgttt ttgagagcca
     2041 gaataaagct attgaggaaa aaaaagccgc ggcaaccgct gctttggttg gcgggattat
     2101 ttcatcagca ttggggatct taggttcttt tgcagcaatg aacaacgcgg ctaaaggggc
     2161 tggtgagatt gctgaaaaag caagctctgc atcttcaaag gctgctggtg cggcttctga
     2221 ggttgcaaat aaagctctgg tcaaggctac ggaaagtgtt gctgatgtcg cagaggaggc
     2281 atccagtgcg atgcagaaag cgatggccac aacaacgaaa gcagccagcc gtgcatctgg
     2341 cgttgcagat gatgttgcga aagcctctga ctttgctgaa gatcttgcag acgccgccga
     2401 gaagacaagc agaatcaata agttgttgaa ttccgtagat aaactgacca ataccacagc
     2461 atttgttgcc gtgaccagtc ttgctgaagg tacgaaaacg ttgccaacaa caatatctga
     2521 gtccgtcaaa tcgactcatg aggttaatga acaacgtgcg aagtcgctgg aaaacttcca
     2581 gcaggggaat ctggagctgt ataaacaaga cgttcgcaga acgcaggatg atatcacgac
     2641 tcgtctgcgt gatataacgt ccgctgtccg cgatctcctt gaggtccaga atcgtatggg
     2701 gcaatcgggt cgcttagctg ggtaattgat catggtcgat acgtttaatg atgaagtgtt
     2761 taattactat cttgaacaaa aagggtacac aatacagaag gagtttcttt gtggcagtgc
     2821 cttttttatc ggatggcgga ttgagacacc ttttttttca ttagcgtaca gactggatga
     2881 acaagaactg attttgtgct cttttgaagc acgtaaccaa acagggctta acggccctgt
     2941 tttatcgctg actcatttgc tcgaagagtt gtatcaccat ttttcgggta ttaagaaaat
     3001 cagcgcgatg aaatctaaga ttggttcaga ttcagaacgt caaaagcgcg aagagttgtt
     3061 taattacttc atcagaaagg gtgccgtgca gcaagaaaca gaagacggaa tttggttcgt
     3121 aatgaacgta aatagttaaa agtttttatt ttattaactt ctgagggaaa tttaatgaat
     3181 ttatctgaaa ttactcaaca aatgggtgaa gtaggtaaaa cgctgagcga ttctgtgcca
     3241 gagttactta atagcaccga tttggttaat gaccctgaaa aaatgttaga gttgcagttt
     3301 gcggttcagc aatattctgc ttatgttaac gtagaaagtg gaatgttgaa aacgataaaa
     3361 gatctggtct caaccatttc taaccgtagt ttttaatttt aatggttaat gatatttctg
     3421 ctaataagat actggtgtgg gcggctgtag ctgcggcaaa ccataagctt cccaaatatg
     3481 cggaagctat cctgaatgta ttcccacaaa ttatacctga taaaaaagat atcgcacatt
     3541 tagaatttat tatcttattt ggatta
//
LOCUS       DAZFKY010000293         3475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFKY010000293 DAZFKY010000000
VERSION     DAZFKY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..747)
                     /gene="sctW"
                     /locus_tag="ACHKCL_004672"
     CDS             complement(<1..747)
                     /gene="sctW"
                     /locus_tag="ACHKCL_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFO5765481.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLS"
     gene            complement(752..2455)
                     /gene="sctC"
                     /locus_tag="ACHKCL_004673"
     CDS             complement(752..2455)
                     /gene="sctC"
                     /locus_tag="ACHKCL_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO5765482.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2452..3201)
                     /locus_tag="ACHKCL_004674"
     CDS             complement(2452..3201)
                     /locus_tag="ACHKCL_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765483.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIHTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          990 a          752 c          563 g         1170 t
ORIGIN      
        1 tgacaactta ttgagaaaca taccaaattc caatgatgaa caactcgcat caagtgcatt
       61 tatatctgag aaaagcgtct ctttgataaa ttttgtcacc aacattctat tttgatagcc
      121 ataactacca atccattcga cataagtatc tacagcatcg tcttctgcca ttaaaaattg
      181 ccgataactc gcccttagta attttggatt taatgacagc gcctggccaa ataactttgc
      241 ttttaatgcc gagttaattc cgctattaat gaatttttta gtagtctctt gattaacttt
      301 tctaagtaat gcttcagcgt ttagccggac attttctgat atttgttttt tacgtaaaat
      361 tgcacgtaac acaactacca gatcgctttc atctgggaat aattttttag caaagctata
      421 cacttcatag tcatgggtag ttgcagcctt aagcgcctcc ataactttgt cggttttttc
      481 tattggctgg tcttccagga catattcttc tccgtccaga aaattggcca gttgttcttc
      541 gtaaattttt ttcttcataa actgcgttaa cgctgctgac atctcgtcag tagcttgtaa
      601 gaaccgagct tctttacctg cgggatcatc cctcatttta ggcatttcag cttcaatgtc
      661 ttcattatct tctgtgataa tgtcctctaa gcgagaaact tccctggctc tttctaatac
      721 accgacatgc tctacatgaa tcgccatttc actatcctcc gacgacttca cgattcaggt
      781 aagtttttat ccatttctgc agtaattcat cgttgatttc tttatttttt gccatttgct
      841 gtgtaatcgc tctggcatca accgcagcct cattcgcatt attcatcatg cgttcatcga
      901 tctctcttgg ctcaataaga aagacacgaa cgatgttatt cgcatttgtt ccttcataac
      961 caaataattt accaataaat ggaatgctgc ctaatattgg gatcttgcga ctttcatagg
     1021 tattcgtatc gcgtgtatat cccccaatca aaagactttt cccttggggc acgcgtgcaa
     1081 tagtacttat aagtgttcta ccaacctgag gcagatcatc aacattggtc ttgtcggaat
     1141 tgatttcatt tccatcttca atattcaaga gtaattcaat ttgatttcgc gcagcaaatc
     1201 gtggtagcac gctaatcatc gtaccatagg ttacttcatc caattccgca gtacgctcgc
     1261 cgactaattt agtatagaaa gtgcggttat tatcaaatat agcaggaata ttctcttgtg
     1321 ttaaaacgac gggacgagca acaacggcgg ctcttctttt ttgtgcaagt gcctgtattg
     1381 ttgcaataaa ctgtgtccca tcaagggtac taattgaacc tgaattatta aatgatgcac
     1441 ttaagctgga tccaattttt atcgtgcccg accagtcagc acctagctgt tctagatcag
     1501 tcttatcaat atctattatc cataaagata attcaatgtg tcgctttgga atatcaagcg
     1561 tagctactaa cttctcgata aaatcaacct gagatactgt tcctttaaca agtaaactgt
     1621 tggtatcagg atatgcaata atttgtatat cgtcagtctc tggcgctcct gctgtcgata
     1681 ttattttctc tacttgtagg tcatcatctt cttcagcccc aggaaaagga ggcatacgtg
     1741 atacgttttt tagttgttga cgacttgaaa tttcagaaag tacgttcacc cctgtgcttt
     1801 gcttaatgtt attatttaac aacgtcgata gtacttttgc catgccagga ataactatct
     1861 tttcgccacg taactcataa gtccggtcat taacgaatgt gttgaccaga tgaattattc
     1921 ctactttatt tcgaccgatc tcaatgccat cgctgttttg ctccattaat ttcgcagcat
     1981 taacgaccag gtcaacataa accggtggcc ccgatacata gaaagtacca ttgccgtccc
     2041 ctttaatttc ataacgagag ttatacagac ccgatttttt taagaaatta ttaaactcat
     2101 tcgtcgatac ctttctcaga ttaatcaatg cattacgcat ctctgatgag tcatagatat
     2161 aaatagcttt gccatcatca taccaaataa gccccatctg tgccgacagg cgttcaatca
     2221 aggcataggg ttctgttaaa tcaaaatttc cgctgatctg tttcttcatg gcaagcttac
     2281 taactattgt aggcttaccc gcatatgatg aaaccgtttc aaaaaaacta cgtagatttt
     2341 ctttatttgc tacgtatcca ttcgctgctg cggcatatgc accaggagtc aataatccat
     2401 taaaaatgca taagactgaa attaatataa tagtaatgcg taattttatt ttcataactt
     2461 tttacatagt tctctcggtg tttttcctaa ccggcttttt acttcggctg aaaagtgtga
     2521 tgaggacgag taaccatatt tatgcgcaat agttgtcata tcactgtttc cttcaatgat
     2581 ttccaacacg gctctggcaa ctctccagcc acataattcc gttttaactt taccacccaa
     2641 tgcataacta cataaacggc gaaaatgtga atacgatacg ccgtaaagct caccaagatc
     2701 atagagtgaa gtatgtatga gcgattgcga taataaataa cgaaccagtt tatagcattc
     2761 tgtttttctt agatgctgaa acaggatttc attaatatta tcgctgctca gataagtggc
     2821 taaaaaccag gcttcacttg caactttact gaatagcaca acttctgatg caaagggcaa
     2881 ggcatcagca aacaattcat ggtgttccct ttccacactc cccgttgatt catctataaa
     2941 agctaataat ttactcagga tatcaatgcg aagtggatga taaattactt tccctgcagt
     3001 tacctcaatt tggcttcggt caatgagcag taatatatct tccactacca ttaatttttg
     3061 atggttatta ccaatcgtaa taacaagggt tacatccttg ttttcatttc cccgtaagaa
     3121 ccacatttca gggtccagta gggttaactt ctgtccttga agcaaaacat tcattggaag
     3181 taacagccct tcttcaatca tgtttcatct ccttattcca tattctcatt ctccataaac
     3241 ttgctataaa aacatgatta aaaagtgatg attactattt ataaaataat gattttgtta
     3301 tgagcgaata gctatcagcc acttacacta gtacgtcatc ctgtccatat acagatttgt
     3361 aactgttgat atgttttact taaacacacc tcgtattact tataacactt gtttggtaac
     3421 tattttcaat acaaatcata tttgttagtt cttcattaac acactgcgag tgttt
//
LOCUS       DAZFKY010000294         3436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFKY010000294 DAZFKY010000000
VERSION     DAZFKY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            219..1874
                     /gene="aslA"
                     /locus_tag="ACHKCL_004675"
     CDS             219..1874
                     /gene="aslA"
                     /locus_tag="ACHKCL_004675"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5765484.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2042..3277)
                     /locus_tag="ACHKCL_004676"
     CDS             complement(2042..3277)
                     /locus_tag="ACHKCL_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5765485.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
BASE COUNT          872 a          906 c          848 g          810 t
ORIGIN      
        1 caataatcaa cgcgatataa taaacctgcc ttacatatca actgcgccag aggtaggatt
       61 gaaaacgctc tcctgatttt taaattcatt tcttggataa ataaataatt tatttttatc
      121 actattattt atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta
      181 cgtcatcatt ttgttgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca
      241 aacttcttgt tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc
      301 cgtcaactgc caccgcgcgc aaaggctttg ccggatacga tcacccaaac cagtatctgg
      361 ttaaaccggc gaccactatt gccgacaaca tgatgccagt aatgcagcat ccggcgcagg
      421 ataaagaacc ccagcagaag ctggcagaac ttgagaaaaa aaccggtaag aaaccgaatg
      481 tggttgtttt cttgctggac gatgtgggct ggatggacgt cggttttaac ggtggcggcg
      541 tggcggtggg taaccctaca ccagatatcg acgccgttgc cagccagggg ctgattttaa
      601 cttcggcgta ttctcaacca agctcttccc caacccgcgc caccattctc accggacaat
      661 actccatcca ccacggcatt ctgatgccgc caatgtacgg gcaaccgggc gggctgcaag
      721 ggttaaccac gctgccgcag ttgctgcacg atcagggcta cgtcactcag gccatcggga
      781 aatggcatat gggggaaaac aaagagtcgc agccgcagaa cgttggcttt gatgatttcc
      841 gtggctttaa ctcggtgtct gatatgtaca ccgaatggcg cgacgttcac gtcaatccgg
      901 aagtggccct gagtccggac cgttctgaat acatcaagca attaccattt agcaaagatg
      961 acgttcatgc ggtgcgcggc ggcgaacaac aggccattgc cgacattacg ccgaaatata
     1021 tggaagatct ggatcaacgc tggatggaat atggcgttaa gttcctcgac aagatggcga
     1081 agagtgataa gcctttcttc ctctattacg gcactcgcgg ctgtcacttc gataactacc
     1141 cgaacgccaa atatgcgggt agctctccgg cacgcacctc gtatggcgac tgcatggtgg
     1201 aaatgaacga tgtgttcgct aatctgtata aagcactgga gaaaaacggt cagcttgata
     1261 acacgctgat tgtctttacc tccgataacg gaccggaagc cgaagtaccg ccgcacggac
     1321 gcacaccgtt ccgtggtgcg aaaggatcta cctgggaagg cggcgttcgc gtaccgactt
     1381 tcgtttactg gaaaggcatg atccaaccgc gtaaatctga cggtattgtt gatctggcag
     1441 atctcttccc aacagcactg gatctggcgg ggcatcctgg tgcgaaagtg gcgaatttag
     1501 tgccgaaaac cacctttatc gatggtgtgg accagacatc tttcttcctg ggaacaaatg
     1561 gtcagtctaa ccgtaaggcc gagcactact tcctcaacgg taaactcgct gctgtgcgta
     1621 tggatgaatt caagtatcac gtcctgattc agcaacctta cgcttatacc cagagcggat
     1681 atcagggtgg attcaccggc acagtaatgc aaacagccgg atcgtcggtg tttaacctct
     1741 acaccgatcc gcaggagagc gactctatcg gcgtgcgcca tattccgatg ggtgtaccgc
     1801 tacagaccga aatgcacgcg tatatggaga tcctgaaaaa gtatccacca cgcgcgcaga
     1861 ttaaatctga ttaagccggc gctatcgctg agatctgcct ttgccggatg cgatgctgac
     1921 gcatcttatc cagcctacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
     1981 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc gaccacaaaa tggccgcttt
     2041 tttacttact caccaccagc aaatgcgcct gcataatgtc gctggccggg cgaccgtgcg
     2101 ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc
     2161 cggcacacaa ataattcagt cccggtttgc cgctggcatc gagcatgaag cggtgtttcg
     2221 ggcagcctcc ccaacatgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct
     2281 taaatttatc ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt
     2341 gctggtgcat attccccagt cgatattgcg gataaacata gtgatcgcag gcgtaaacgt
     2401 cgccattgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac
     2461 ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga
     2521 cgtcgcgttt aatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc
     2581 gcacagacca ttccgtcagt tccccctgaa tatcaccagg cgcatgtagt ttcagtcctt
     2641 cgcgggcagt cgtttcatca gccaggcgct cgaccaccgg aataaactgg atgaattcga
     2701 ctcccgcatc gcacaaaaaa tcataaacct gtaacggttg ctgcgcgctg gtgcggttga
     2761 cgcagaccag cacgttatag tcgacatgat gtttttgcag gagcgtcagg gcacgcatca
     2821 ccagtttatg cgtgggtctg ccgccttttg tcacgcgata ttgattgtgg atctcagccg
     2881 gaccatccag cgataaccca acaagaaaat gattttctgc cagaaatgca caccattcat
     2941 catcgagcag tacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccatatt
     3001 tcgcctgtag cgccacggca cggcggtaaa actccagccc gagtagcgtt ggttcgccgc
     3061 cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat
     3121 acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt
     3181 tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccgctc ggtttcgcca
     3241 tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cgctccttgc ctgaagtcat
     3301 ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat
     3361 tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa
     3421 aattggtcgg cgagag
//
LOCUS       DAZFKY010000295         3430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFKY010000295 DAZFKY010000000
VERSION     DAZFKY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(669..866)
                     /locus_tag="ACHKCL_004677"
     CDS             complement(669..866)
                     /locus_tag="ACHKCL_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765486.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(932..1202)
                     /locus_tag="ACHKCL_004678"
                     /pseudo
     CDS             complement(932..1202)
                     /locus_tag="ACHKCL_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1507..1695
                     /locus_tag="ACHKCL_004679"
     CDS             1507..1695
                     /locus_tag="ACHKCL_004679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765487.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            2023..2217
                     /locus_tag="ACHKCL_004680"
     CDS             2023..2217
                     /locus_tag="ACHKCL_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765488.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2560..2688
                     /locus_tag="ACHKCL_004681"
     CDS             2560..2688
                     /locus_tag="ACHKCL_004681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765489.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2998..3333)
                     /locus_tag="ACHKCL_004682"
     CDS             complement(2998..3333)
                     /locus_tag="ACHKCL_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765490.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1021 a          777 c          745 g          887 t
ORIGIN      
        1 attattttta tgtgagctta tgcacaaaaa taaccaagta tattatcttc tggtagagat
       61 tcaggataca gtttgtagga gctttctttt ttgaacaatg atttgcagga cagttatgct
      121 aaactagcgg aatcagtatc acaacaataa atgactgatc atacctgaaa tttgaattaa
      181 caggcatcat gaatgtcaat agtctaagcg gtgactactt atatcgcttg cataaatact
      241 aaacactgac tagaaaagta ttggataacc ccgtcactaa aagtaacgct tcggttattt
      301 gctcggaatg tattaacgcc aagattgtta gccgttacgc cttatatcac tggcgctgac
      361 aaatgtttct tttctaattt actgaaccgg cgcagacacg caggaaagca cgccggcacc
      421 ggtgtaatgc cagttctaat caataagaat ggaatgacga atcagtcagg aagggataac
      481 aacccgctgc tgaacggaag gtatcagcag gaagatgaac gggaatatcg ccagcaccaa
      541 gggcagggcc gggttccaca ataccagcgc tggcattttg cacattgcca gcgtaccggc
      601 attacaacac gtttcatgct gctgcccccc aggattcctc gattcgttgc tacatccact
      661 gctctacttc acttccactg ctccacttca cttttgtacc agcggctgct gcgccccagt
      721 ttgatgggtt tagggaaatg gccttcactg atcaacttgt aaaaccattt gtcggtcata
      781 ccggtgaaac tggtgatata gttcatgtcg atcagggaat tatcggcacg cagaaatacg
      841 ctgtcgtggc ttgttgtagt caacatggtc agaatgtcct gtgagattta tgaagattgt
      901 gcgaatgcgg gaaatctact gagctgtgct ttcagaactg gcctgttacg ggataccagg
      961 gattaccggc ggggtaacgg gcttccggat catacacacc acgattatcg cggacaaaat
     1021 cactgaacgc ccatatcacc tctttaagta tgtcttcgca gtccggtaaa tgacgatcca
     1081 gcgccacatc ccgggtggta cagctttgat gcgcccggtg acacaaagcc cggattgttc
     1141 cagacatcct gaatcaaacg ccccagatta ggggcgtcga aatatgcctc tctgaccatt
     1201 atattccggc gtataggtag caggtctgaa gtgacaatgc gtcacctgac gttaaaagtc
     1261 acccaagatg accccagtgg gggcaacaag tataaaaccg ttttttgatt aagtggtcgg
     1321 atatgaaaag gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa acacacaaca
     1381 gtatcaggtt atttatgttt atacagtgcg ttccaaactg acctgaataa ttacgcaagt
     1441 tgcaacaata aaacattttt gccgtagtgt gaggtactcc ggtaaattgc ttcccaggtg
     1501 cagacaatga ctaacgaaga actacaggca atcgcattat ccatgcttga gcgccagcgc
     1561 gaaaaagtac accagcaacg agagtctttg cttaacccat ctccattcac caggccaaaa
     1621 cgattggagc ttgaagcatt cttggacaaa tacccacggc ggcttaaatc aggcaaaacc
     1681 agaccaccag gctgatagct cacagtttgt tctccgattc aataaaacgg ataagaacca
     1741 tcagcgcggt agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta
     1801 tcaccagaag acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt
     1861 atcgaacagc aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct
     1921 cctttagttg acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt
     1981 tcattagcac agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata
     2041 aaaaggctgg agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt
     2101 gccgcgctgc cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat
     2161 ggcaaaaaaa tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa
     2221 caatctgcat attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct
     2281 ttatatccac tgacgagttt gccggtcatt ctggtatcat gcagatattt acggcgtaca
     2341 agcacactag catgacattg taataaggcc tgagcctaac gctttaatgt gtgtcataca
     2401 ctcagtggcg taatcgttga caatatggtg catgtaatcg taagcgtcca gcgaaatcca
     2461 tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg atctgcgccc
     2521 aatatcctga gccggaactt tgacaaaggc caacgaaaaa tgggagcgga tgttacagag
     2581 ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt taaccgggaa
     2641 attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata cgatgctata
     2701 cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatatgga tcagggttgg
     2761 caatatcgaa ttgcaggcta taggcaaagt taaagcccca tggagtagca caaaatatgc
     2821 cgcacaaagg aaacggtctg aataacgcag tgatgaagaa cttcttcagc acactgctaa
     2881 aacgcatagt gatcgagcgc tgattctggc gaacaactga actaatacat cagaatctgc
     2941 attatgttaa ataaatataa aaagatggtt taaatacccc gttacttttg acttacacta
     3001 tacggtatcg catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac
     3061 aatactgaat gcgtacgacc acagtattct ggctcctgtg tggttatgct ttaattctgc
     3121 gttccgggca gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct
     3181 tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat
     3241 agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt
     3301 ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa
     3361 aataagcact actccataca ggataattag aggtcagttt gattattcac aattcatcat
     3421 cagcattttc
//
LOCUS       DAZFKY010000296         3406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFKY010000296 DAZFKY010000000
VERSION     DAZFKY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..162
                     /locus_tag="ACHKCL_004683"
     CDS             <1..162
                     /locus_tag="ACHKCL_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5765491.1"
                     /translation="KRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQES
                     ALACWGIDR"
     gene            436..1131
                     /locus_tag="ACHKCL_004684"
     CDS             436..1131
                     /locus_tag="ACHKCL_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFO5765492.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1226..1357
                     /locus_tag="ACHKCL_004685"
     CDS             1226..1357
                     /locus_tag="ACHKCL_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765493.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            1580..1765
                     /locus_tag="ACHKCL_004686"
     CDS             1580..1765
                     /locus_tag="ACHKCL_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5765494.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPS
                     PDWQTPLNGIISSSENG"
     gene            2166..2492
                     /locus_tag="ACHKCL_004687"
     CDS             2166..2492
                     /locus_tag="ACHKCL_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5765495.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(2624..2824)
                     /locus_tag="ACHKCL_004688"
     CDS             complement(2624..2824)
                     /locus_tag="ACHKCL_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5765496.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2866..3231
                     /locus_tag="ACHKCL_004689"
     CDS             2866..3231
                     /locus_tag="ACHKCL_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5765497.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          846 a          731 c          940 g          889 t
ORIGIN      
        1 aaacgaatta atgcaggaga tcgaaaaggt gcctgcgaag cgattcgctg gtggattaag
       61 gacggtggca gagactgccg tattcgctca aataactgtt atggtcaggt atcccgtcgt
      121 gaccaggaga gcgcgctggc gtgctgggga atcgacagat aagcagaata ttttgctgaa
      181 aaatgacgtt ggccagcgcg ggcggataac acgaaatcct gcgaactggc aaaatgtaag
      241 tgaataaagt taggcagatt atttcacgca gaggcaccgt aatggtgcct ttgtcatttc
      301 tgcgcttcgc acaagcgtaa ataaaccaaa gaacctttca ggatgagccc tggtggataa
      361 ccggcagtgg tctggttaac cctctttggg ctggttattc ctgtgcgcag ggttcatcac
      421 taaaaggaat caaccatgaa agagatgatt tctgtcgatc atgaaatatc catgagtagt
      481 ctggattttc tgaataacat tattaatcca gcccgggcag aagccggaga agtccctcat
      541 gaaccgcgta agtttcttgc aaaaattgag gatgagctag agcttgatgg aaccggaaaa
      601 aaattccggt taaacaataa ccagacaaga acggcatact atgatctgga ttttgaccag
      661 atgatgctcg ttggcatgag ggagtcaaag gccgttcgtc gttctgtgct ggcaagactc
      721 aaagcgatgc atggtattca gatcccccgg actttacctg aggcgttgcg atttgcggca
      781 aaactggctg aacagaaagc agtgctggaa aatcaactgg caatagcagc gccgaaggct
      841 gaatttgttg ataactatgt tgaagcatct ggtctgatgg gattccggga agttgctaag
      901 ttactcggta tcaaagaaac cgatttccgg ctgtttttgt tggagaacgg aataatgtat
      961 cgcctcgctg gaaaaatgac gccttactcg catcacctgg atgcggggcg gtttagcgtg
     1021 aaaacgggcg aggcgggcaa tggtcatgct tttacgcagg ttaaattcac cccaaaaggt
     1081 gttcagtgga ttgctggtct gcttgctgca tggagagcta ccgcagcatg aagatgataa
     1141 aaacggactg gaaatttttg ctggtctggc tgattccgtt tttatgggtg gttgcccggt
     1201 taattactgc tattaagggg taaagatgtc agacaaactc ataatgctgg cgaagggcct
     1261 ttgtgtaatc gtcggtattt cattttcact aatgctggtt gttctttttc tttccataac
     1321 ctggatggcg ttgacttcgg cagggctggt ggggtgagca taaaccgaat gctttccgcg
     1381 tttaccgtta ttctgctggt ggtctgtggt gcgcttagtc tggggctgaa tcattaccgc
     1441 gataacgtca tcacctacaa agcgcagcgc gataaaaaag ccagagagct ggagctggca
     1501 aacgcaacca ttactgatat gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa
     1561 tactcgaggg aattagccga tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc
     1621 gctggtcgta agcgcctgcg gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc
     1681 ggcacctccg gcgtggataa tgcaaccgtc ccccgactgg cagacaccgc tgaacgggat
     1741 tatttcatcc tcagagaacg gttgatgaca atgcagaagc agctggaagg ggcacaggac
     1801 tatatccgca ctcagtgcct gaactaagtt ttgctgatgc gccgtatcgt cgctgtattc
     1861 cctcattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggaga
     1921 atcaaaaacg atacgcaccg gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat
     1981 ggtcgctggt tcggtgcgat ggtggaagaa accggacgat gtgttacctc gcaagacctg
     2041 ttatgtcatg tgtctgattt gtgatttaag tcggataatt gtcgttgcca ttaagcagag
     2101 gattgatggc cgacagggtg gcattgttag aataagactt attcttatct gtgccgggaa
     2161 tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt atgggctgta
     2221 cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa tatccggcct
     2281 atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt ggtgctgatg
     2341 gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt gatgagcagg
     2401 ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa ggcgtgaaga
     2461 aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta attatcactc
     2521 tgttgttatt ctgtacattc cggctgggta agtcttgttc cgccgggtat gaagatgaaa
     2581 tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg
     2641 tcatgctggc cggacgcaga tgcgttgcat ctgttgccag ccttctcctg caggcttcaa
     2701 taacccacgc tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa
     2761 tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg tttttcgatg
     2821 acatatttat ttcctttact gattgccata tgacggggat tttacatggc tgagcttcgt
     2881 acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct gaagggtgaa
     2941 cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg gctcagggat
     3001 gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga actcgatcac
     3061 cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac gctctgtacc
     3121 gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga cccgacgctg
     3181 ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta acccgagcgg
     3241 gggggtatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct ctcacgcaga
     3301 gaaaaaattc ccgtttcagg gcagttaaca tgttaactgg ctgtccgggc atttttgcgg
     3361 tttttatctt tattattcag tttgttgtgc gaaaaaaatg ttaact
//
LOCUS       DAZFKY010000297         3390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFKY010000297 DAZFKY010000000
VERSION     DAZFKY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACHKCL_004690"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACHKCL_004690"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5765498.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACHKCL_004691"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACHKCL_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5765499.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1510..2394
                     /gene="yhdJ"
                     /locus_tag="ACHKCL_004692"
     CDS             1510..2394
                     /gene="yhdJ"
                     /locus_tag="ACHKCL_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5765500.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            2478..2657
                     /locus_tag="ACHKCL_004693"
     CDS             2478..2657
                     /locus_tag="ACHKCL_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5765501.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2660..3322)
                     /gene="acrS"
                     /locus_tag="ACHKCL_004694"
     CDS             complement(2660..3322)
                     /gene="acrS"
                     /locus_tag="ACHKCL_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5765502.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          926 a          789 c          803 g          872 t
ORIGIN      
        1 attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaat
     1441 tgcttgatta aaaggcgcta ctcggcatgg ggaagcgcct tttttatagg tgtcacaaag
     1501 ggagtgacca tgagaacagg atgtgaaccg acccggtttg gtaatgaagc taagaccatt
     1561 attcacggtg atgcccttgc cgaacttaaa aagctaccta ctgaaagcgt cgatctgatc
     1621 tttgccgacc caccgtataa catcggtaaa aattttgatg gtctgatcga agcctggaaa
     1681 gaagatctgt ttatcgactg gctgttagaa gtgattgcag agtgccaccg cgttctgaaa
     1741 aagcagggca gcatgtacat catgaacagt acggaaaaca tgccctttat cgatctccag
     1801 tgccgcaagc tttttaccat caaaagtcgc atcgtctggt catatgacag ttctggagtg
     1861 caggcgaaaa aacactacgg ctccatgtac gaacccatcc tgatgatggt gaaagacgca
     1921 aagaactaca cattcaacgg tgatgctatt ctggtagaag ccaaaaccgg atcgcagcgc
     1981 gcgttgatcg attatcgcaa aaatcctcca cagccttaca atcatcaaaa agtaccgggt
     2041 aacgtctggg attttccgcg cgtgcgttat ttgatggatg aatatgaaaa ccacccgacg
     2101 caaaaaccgg aagccttact gaaacgcatt attctcgcct cttccaaccc aggcgatatc
     2161 gttctcgacc cgtttgctgg tagctttact accggtgccg tagccatcgc cagcggacga
     2221 aaattcattg gtattgagat caacagcgag tacatcaaaa tggggcttcg acggctggat
     2281 gtcgcgtcgc attactctgc ggaagaactg gcgaaagtga aaaaaagaaa gaccggcaac
     2341 ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc tcattacaaa gtaaaggatg
     2401 tgtaagcctg gtttgcagat tattcatttc gtgtatattt cctgccagac ttggttaaac
     2461 atgcacaggc tcaggtaatg attcgcaagt attggtggct cgtcgttttc gctgtcttcg
     2521 tttttctgtt tgatacttta ctgatgcagt ggattgaact gctggcaaca gaaacagaca
     2581 aatgccgcaa tatgaactca gttaatccac taaaactggt taactgtgac gaactgaatt
     2641 ttcaggacag aatgtgaatt tacatgacac ttaattcatt cgtttgatga attaatttcg
     2701 ttatgttttc atctggcatg aacattctta atacgttatc gaccagagcg ggggcttgtt
     2761 tataaagatc ataacccgcc atattcatta accagttttg aacaattccg ctgaaggcac
     2821 catcaataat aatcatcaca acatctaaat cgaggttatt tgctacacaa ccttgttgct
     2881 gacacgcctg caatacttcg cggagagtct gcggattaaa gcccatcttt tcgcgtatca
     2941 ctccctcggc cagcatctca tcattaaatt cacatttgtg atataagatt ttcagcaacg
     3001 cctgctggcg gggaattttg gcaatatatt gcaagccgac aatcaatttt tcacgcaatt
     3061 gttgaaacgg gtcatgctct aatccagccg tcaagtgttc ctggattaac tcccgcaatg
     3121 aaggctgttg caaccacatc tcattaaaca gttgggtctt gttctcgaag tgccagtaga
     3181 tagcgccacg cgtaacgtta gcggcatcgg caatgtcgtt gagcgtcgtc ttgcttacgc
     3241 catgttgcgc aaactgggcg atggcagttt caatcagttc ttgccgggtc ttcagagctt
     3301 cggctttggt tctttttgcc atgattaatt attcaggaaa taaatatctt cgacacagag
     3361 tgagaaaata gcgaaggtta atctatcacc
//
LOCUS       DAZFKY010000298         3388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFKY010000298 DAZFKY010000000
VERSION     DAZFKY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..423
                     /locus_tag="ACHKCL_004695"
     CDS             <1..423
                     /locus_tag="ACHKCL_004695"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5765503.1"
                     /translation="AASDKNALCAHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVE
                     KAAEQCIQQRQTVVMLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGQGVRRAA"
     gene            420..602
                     /locus_tag="ACHKCL_004696"
     CDS             420..602
                     /locus_tag="ACHKCL_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5765504.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            877..1554
                     /locus_tag="ACHKCL_004697"
     CDS             877..1554
                     /locus_tag="ACHKCL_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO5765505.1"
                     /translation="MAVQISVENLSPVTYNQIPVITTELLAHLYGTKIKNISDNFLNN
                     TTRFMPGKHYFKIEKNELREFKNRPETIGLVGKNARSLILWTERGAARHAKMLETDQA
                     WDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVWLWDYANRSQALFRELYPAL
                     KLIQSGYSGICHDYGYKFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKEL
                     PPSLHRY"
     gene            1629..2351
                     /locus_tag="ACHKCL_004698"
     CDS             1629..2351
                     /locus_tag="ACHKCL_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5765506.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            2351..2956
                     /locus_tag="ACHKCL_004699"
     CDS             2351..2956
                     /locus_tag="ACHKCL_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5765507.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            2953..3147
                     /locus_tag="ACHKCL_004700"
     CDS             2953..3147
                     /locus_tag="ACHKCL_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5765508.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            3140..>3388
                     /locus_tag="ACHKCL_004701"
     CDS             3140..>3388
                     /locus_tag="ACHKCL_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5765509.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMA"
BASE COUNT         1018 a          778 c          874 g          718 t
ORIGIN      
        1 gcagcgagcg acaaaaatgc tctgtgcgct cactggctaa ctgaggccga cgacgcgctc
       61 aattctgagt gggtaagcca cggtgcaatc tggaataacc caccgtacag caatatcagg
      121 ccgtgggtgg aaaaagccgc tgagcagtgc atacaacagc gacagacggt agttatgctt
      181 gtgccagagg atatgtcagt cggatggttc agcaaggctc tggagagtgt tgacgaagtt
      241 cgcatcatca ctgatggacg gattaatttt atcgaaccat cgacagggct ggagaagaag
      301 ggaaacagca aaggctccat gctgctgatt tggcgaccgt tcatcagtcc tcgacgaatg
      361 tttactactg tatccaaagc ggcattgatg gcgatcgggc agggcgtcag gagggcggca
      421 tgaggcgaca gcgacgaagt atcaccgaca tcatctgcga aaactgcaaa taccttccaa
      481 cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga atctgacgta aaaaccttca
      541 attacacggc tcacctgtgg gatatccggt ggctaagaca tcgtgcgagg aaaacaaggt
      601 gattgacgcg atgatttatt cggggctata ttcctcacgc gccagcaaaa tctggcgtcg
      661 ggattagcac cccggatatc gaaacggtgc ataaccgcgc tggcggtttt ttatgcgcta
      721 agcacagtca cattcgcgat ttatggcggg ctgtgtgggg gagccgaaag gctcgccgga
      781 tgtttcgacc ggtagtgcta accccgcaca gttcgccacc acgatgatta gcacctgacg
      841 gtggcgaggt aaaaattatc gaaacgcgag gtcattatgg ctgttcaaat ttctgtcgaa
      901 aacctttccc ctgttaccta taaccagatc cccgtaatta ctactgaact gttggctcac
      961 ctttacggaa caaaaatcaa aaacatttct gataactttc tgaacaacac gacgcgattc
     1021 atgcccggaa agcattactt taaaattgaa aaaaacgaat tacgcgagtt taagaacaga
     1081 cccgaaacaa tcgggttagt tggtaaaaat gcccgctccc tcatcctctg gacagaacga
     1141 ggcgcagccc gtcacgcaaa aatgctcgaa actgatcagg cgtgggatgt gttcgaaaaa
     1201 ctggaagact gctatttcag ccagtgcgag aaaaatactg gcaaacaaga gaagaagctc
     1261 aacgggcttt ccgcaaaaga aacagacagc cttgtatggc tgtgggatta tgccaaccgc
     1321 tcacaggcat tgttccgtga gttgtatccc gcattaaaac tgattcagtc tggctattcc
     1381 ggcatatgcc acgactacgg ctataagttc tcgtatatca tcgggagggc gaggggcgtt
     1441 ttaattaatc acacgcggga tatagatatt tatgagcctg acgggccgac gaaccttctg
     1501 gcatgggaaa ggcttaagaa caaagagttg ccgccttcac tgcatcgcta ctgacaattg
     1561 acaacttaac aaacccagct tcggctgggt tttttattgc tgaattttca atgtgagagg
     1621 acatgacaat gaatgagctg ataaatagca atgccatcaa aatgacaagc attgaaatcg
     1681 ctgagttggt gggaagccaa cacggtaatg tcagaatatc aatagaacgt ctggcaaagc
     1741 gtggggtgat tcaacttcct tcaatgcaaa aagttgaaaa taaacaaaca attagcccta
     1801 acaaattcac aagcgtgtat atattcgaag gcgaacaagg taagcgagac agcattattg
     1861 tcgtcgctca gttgtcgccg gaattcaccg ctcgccttgt tgaccgctgg cgagaactcg
     1921 aaggggcaac cgcgaaaata ccacaaacct tttctgaggc attgcgcctt gcggccgacc
     1981 ttgaagacca gaaggctgaa ctggagaaac agcttgctct cgcagcacct aaagttgagt
     2041 ttgccgatcg cgttggcgag gccagcggaa ttttgattgg aaactttgca aaggttgttg
     2101 gtattggtcc aaacaaactg tttgcgtgga tgcgcgatca caaaatcctt attgcttcag
     2161 gttcccggcg aaatgtgcca atgcaggaat atatggagcg cggctatttc acagtgaaag
     2221 aaacagcggt caatacaaat cacggaatac agatatcgtt caccacaaaa atcaccgggc
     2281 gtggtcaaca gtggctgaca agaaagctgc tagataacgg aatgcttaaa gtaacagggg
     2341 aggctgctta atggctaaac cagcgcgaag gaaatgcaaa atctgtaagg aatggtttca
     2401 cccggcattc tcaaatcagt ggtggtgctg cccggaacac ggaactcaat tagcactcga
     2461 acgacgaagt aaagaacgcg aaaaagcgga aaaagcagca gagaagaaac gacgacgaga
     2521 ggagcagaaa cagaaagata aactgaagat tcgaaaactc gccttaaagc cccgcagtta
     2581 ctggattaaa caagcccaac aagccgtaaa cgccttcatc agagaaagag accgcgactt
     2641 accatgtatc tcgtgcggaa cgctcacgtc tgctcagtgg gatgccggac attaccggac
     2701 aactgctgcg acacctcaac tccgatttga tgaacgcaat attcacaagc aatgcgtggt
     2761 gtgcaaccag cacaaaagcg gaaatctcgt tccgtatcgc gtcgaactga ttaaccgcat
     2821 cgggcaggaa gcagtagacg aaatcgaatc gaaccataac cgccatcgct ggactgtcga
     2881 agaatgcagg gcgatcaagg cgaagtatca gcagaaactt aaagacctgc gaaacagcag
     2941 aagtgaggcc gcatgacgtt cacagtaaaa accattcctg acatgctcgt tgaggcatat
     3001 gaaaatcaga ccgaggtagc cagaatactg aactgtagtc gcaacacggt cagaaaatac
     3061 actggcgata aagaagggaa aagacacgct atcgtcaacg gtgttcttat ggttcaccgc
     3121 ggatggggta aagatactga tgcgtgatat ccggcaggtt cttgagcgct ggggggcatg
     3181 ggcggcaaat aactatgagg atgttacatg gtcgcccatt gctgccggat ttaagggact
     3241 gatccccgaa aaagtaaaat cacgtccaca gtgctgtgac gatgatgcga tggtgatatg
     3301 cgggtgcata gcccgccttt accggaacaa tcgcgatctg catgacttgc tggttgatta
     3361 ttacgtgttg ggggagacgt tcatggcg
//
LOCUS       DAZFKY010000299         3385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFKY010000299 DAZFKY010000000
VERSION     DAZFKY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..845)
                     /locus_tag="ACHKCL_004702"
     CDS             complement(<1..845)
                     /locus_tag="ACHKCL_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5765510.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHKCL_004703"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHKCL_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ACHKCL_004704"
     tRNA            complement(1335..1411)
                     /locus_tag="ACHKCL_004704"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ACHKCL_004705"
     tRNA            complement(1425..1501)
                     /locus_tag="ACHKCL_004705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ACHKCL_004706"
     tRNA            complement(1509..1584)
                     /locus_tag="ACHKCL_004706"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
     gene            complement(1613..2326)
                     /locus_tag="ACHKCL_004707"
     CDS             complement(1613..2326)
                     /locus_tag="ACHKCL_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765511.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2464..2660)
                     /locus_tag="ACHKCL_004708"
                     /pseudo
     CDS             complement(2464..2660)
                     /locus_tag="ACHKCL_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(2947..>3385)
                     /locus_tag="ACHKCL_004709"
     CDS             complement(2947..>3385)
                     /locus_tag="ACHKCL_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO5765512.1"
                     /translation="KKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLP
                     EFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYSGGALLVLFA
                     TLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          970 a          881 c          736 g          798 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatgaa aatcaataat
     1621 caagcccttg cctggataca tatctgtctg gcgggaagcc ataatagcgg tgaaatacag
     1681 aaataaagta ggaccagctt gaataaccgc atttttctgc tacagcctgt ccatatccat
     1741 gccgggaaca taacatattg acagcaacac gcatccgctc ttccagcaac aagcgactga
     1801 acatgtgccc ttcatttttc agttttatct ttaacaaact ctcactcata tgcaggcgta
     1861 acgcaatcgc accaagcgtc cagcttgctg atatatctgt ctgaattatc gccctgactt
     1921 tggcacttat actggataaa catccactta aaaataataa tatccgttca tctgattcaa
     1981 acagcgacag gcatgccatc ataagaaaca tatccgtggt atctccggaa aatctctggc
     2041 tggtaattaa agcctcagcc aacgcagggt tgttgggttc cagtgacaga taaagcggaa
     2101 cgtcagtcag atgagttctt gtcagcttat gctgaatttc cagatattga cttactatgg
     2161 aatggtttat atcgaaaatt ttaactttgc cataatgcat aaggaaaagc gccctgatgc
     2221 atttggtggc cagaacgact gagccgggct taagtgacaa cgtatccttt tcaagaaaaa
     2281 tattaattgg ggagcaaacc atgataactg aacagacaac aaccattata attttacttt
     2341 cattagcaat tggttagttc aattatagcc ccaaaaggta aattatcatc aacacataag
     2401 caaaggactg acaggtgccg ctaacaccca ccagccgccc atttaccaca aataaaaaag
     2461 ccttcaggac tgaaggcgtc tgtaacaacc gaactgatag tctgccagac ccgcataacc
     2521 agctgggtca gtattaactg gcagcgctcg cgtgaaaggt acgtattctg cgcaatctcc
     2581 ccgaccgtcg ccggttcggt gacgcttaat tcatcaaaca caactctggc ggtttctgtc
     2641 atatcctgct gtttcagcat gtctttttac cctttccggt taacgtgaca caccaataac
     2701 tcttgtcgaa aaagccagca agctgaaaga cctgtattaa taactaccag cacatttaac
     2761 gcactgcgct actttgcggg cacaaaaaac ccgctcagag gcggggtcaa gctatgcggc
     2821 gaaataacca ctcttaacag catacctgat tttttacgta cgtaaatgct ttgccgtgca
     2881 tatttttcat gcaaatgtca cgtcctgcta tttttcagtc ttataaattt aaaaccatag
     2941 aaaaaatcaa ttatgtttta aaaatggata ggtaaagaat aacaagtgac acagattcaa
     3001 accaaaatgg aaaagggtgg caacccacaa acgcccactc cacatccatg acaatccata
     3061 cacaacacca gataacgtgg caaataatac aagtaaagca ccacctgaat agtgataaaa
     3121 accaaacaac aaagccgcca caattaatgc aaccaatgga gacgttactt ctgatagccg
     3181 tgattgaata taccctctaa ataatgattc ctctgccaga gacacaaaaa acaaattagc
     3241 caatataaac tctggcaacc actcaggaaa atgaatctct ggctttaatc caccaaaaaa
     3301 aacagccaaa aacaggataa gaggaacaga gagcgacaga gccccccact tccacaaaga
     3361 cacttctgat tttacttctt tttta
//
LOCUS       DAZFKY010000300         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFKY010000300 DAZFKY010000000
VERSION     DAZFKY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..390
                     /locus_tag="ACHKCL_004710"
     CDS             31..390
                     /locus_tag="ACHKCL_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5765513.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHKCL_004711"
     CDS             399..929
                     /locus_tag="ACHKCL_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5765514.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHKCL_004712"
     CDS             1171..1368
                     /locus_tag="ACHKCL_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5765515.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHKCL_004713"
     CDS             1519..2577
                     /locus_tag="ACHKCL_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5765516.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHKCL_004714"
     tRNA            2618..2693
                     /locus_tag="ACHKCL_004714"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHKCL_004715"
     tRNA            2774..2850
                     /locus_tag="ACHKCL_004715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
BASE COUNT          861 a          731 c          954 g          816 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aa
//
LOCUS       DAZFKY010000301         3316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFKY010000301 DAZFKY010000000
VERSION     DAZFKY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2667
                     /locus_tag="ACHKCL_004716"
     CDS             <1..2667
                     /locus_tag="ACHKCL_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5765517.1"
                     /translation="GTLNDFLGAMTEDDVRPEALRRFELMVEEVARNASAVAQNTAAA
                     KKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASK
                     SAAAAESSKSAAATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSARDASASK
                     EAAKSSETSAASSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAAGSKTA
                     AALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAK
                     TSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTK
                     ATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSAT
                     NSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEMLAATPKSV
                     KAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSA
                     RKVNGKALSSDITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGA
                     GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAI
                     GNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTG
                     ASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVA
                     IGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2667..3248
                     /locus_tag="ACHKCL_004717"
     CDS             2667..3248
                     /locus_tag="ACHKCL_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5765518.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ASLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
BASE COUNT          906 a          850 c          914 g          646 t
ORIGIN      
        1 ggtacgctga atgattttct tggtgccatg actgaggatg atgtccgtcc ggaggcactg
       61 cgccgttttg agctgatggt ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac
      121 acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga ggcggcaacc
      181 catgcgactg atgctgcgga ctcagcacgc gcagccagca cgtcagccgg acaggccgcg
      241 tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc tactgaagca
      301 tcaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggctaccag tgccggtgcg
      361 gcgaaaacgt cagaaacgaa tgcggcagtg tcacaacaat cagccgccac ttctgcatcc
      421 accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc ggcttcaaaa
      481 gaggcggcaa aatcatcaga aacgagcgca gcctcgagcg ccagtagtgc agcctcctcg
      541 gcaacggcgg caggcaattc cgcgaaggcg gccaaaacgt ctgagacaaa cgctaagtcc
      601 tctgaaacgg cagcagaaca gagtgcctcc gcagcagcag gctcaaaaac agcggctgca
      661 ttatctgcca gtgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc
      721 ggaaaatcgg cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa ggctggcgaa
      781 gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac atccgaaacg
      841 aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtcagcc
      901 agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacaa
      961 gcgtcagcag cgaagagcag cgccacgacg gcatccacga aggcgacaga ggcagctggt
     1021 agtgcgacgg cagcagctca gagcaaaagt acggcggaat ctgcagcaac gcgcgctgag
     1081 acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg
     1141 acgaaaaagg ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg
     1201 gcaacgccaa aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag
     1261 gatgcaacga cagcacagaa agggatagtt cagcttagca acgcgaccaa cagcacatct
     1321 gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa
     1381 tatactgctc aggacgctac gacagcacaa aaaggaattg tccagctcag tagtgcaacc
     1441 aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat
     1501 gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc atcggatata
     1561 acactgacgc cgaaagatat tggtacgctt aactcaacaa caatgtcatt cagcggtggt
     1621 gctggttggt tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttcaatt
     1681 acgttgattg gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa
     1741 cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc
     1801 acatcgacag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt
     1861 tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat
     1921 gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga agggttaacg
     1981 gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca
     2041 atcccgtggc catcagatac cgttccgtct ggctatgccc tgatgcaggg gcagactttt
     2101 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     2161 cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
     2221 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     2281 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcacacgcac
     2341 agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca
     2401 gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg
     2461 aatacaagtg ctgcaggagc gcacactcat agtgtcagcg gcacagctgc atctgcaggt
     2521 gctcacgcac atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat
     2581 ggacacacca tcaccgttaa cgctgcgggt aacgcggaaa acactgtcaa aaacatcgca
     2641 tttaactaca ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2701 taaaaattta taatctgctg accggaacta atgaatttat tggtgaaggt gacgcatata
     2761 ttccgcctca tacaggtctg ccagcaaaca gtacctatat tgcaccgcca gatattccgg
     2821 ctggcttcgt ggctgttttc aacagtgatg agtcatcgtg gcatctcgtt gaagatcatc
     2881 ggggtaaaac ggtttatgac gtggcttccg gcgacgcgtt atttatttct gaactcggct
     2941 cattaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3001 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3061 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3121 atctggaaat cgcaacggag gaagaagcct cattgctgga agcctggaaa aagtatcggg
     3181 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct gtaatacctg
     3241 aagtttaaaa taaaagcccg cttaaaatat cgcgggcttc gaaatataaa tgtattttca
     3301 gaggctagag cttagg
//
LOCUS       DAZFKY010000302         3291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFKY010000302 DAZFKY010000000
VERSION     DAZFKY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>3291
                     /gene="rhsC"
                     /locus_tag="ACHKCL_004718"
     CDS             <1..>3291
                     /gene="rhsC"
                     /locus_tag="ACHKCL_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFO5765519.1"
                     /translation="KVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMP
                     ADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAA
                     LWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDR
                     FGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAF
                     PDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGK
                     QVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITI
                     TDSLNRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPD
                     VVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDI
                     TRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAV
                     HREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWG
                     KAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHH
                     DLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHR
                     VTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWL
                     TYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQY
                     DRDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLP
                     DPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHY
                     DSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWY
                     GWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGED
                     GGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKI
                     HLYHCDHRG"
BASE COUNT          734 a         1009 c         1034 g          514 t
ORIGIN      
        1 aaggtccttc ccggtgaaac cgacatcgcc ctgcccggcc cgctgccgtt catcctctcc
       61 cgcacctaca gcagttaccg gacaaaaacg cccgcgccgg tggggagcct cggccccggc
      121 tggaaaatgc ctgcggatat ccgcttacag ctgcgcgata acacactgat actcagtgat
      181 aacggcggca gaagcctgta ttttgagcac ctgtttcccg gtgaggacgg ttacagccgc
      241 agcgagtcac tctggctggt gcgcggcggc gtggcgaaac tggatgaagg tcaccggctg
      301 gccgcactct ggcaggcgct gccggaagaa ctccgcttaa gtccgcatcg ttatctggcg
      361 acaaacagtc cgcaggggcc gtggtggctg ctcggctggt gtgagcgggt gccggaagcg
      421 gatgaggtgc tgcctgcgcc gctgccgccg taccgggtac tgaccgggct ggtggaccgc
      481 ttcgggcgca cacagacgtt ccaccgcgaa gccgccggtg aattcagcgg cgaaatcacc
      541 ggcgtgacgg atggtgccgg gcgtcacttc cggctggtac tgaccacgca ggcgcagcgg
      601 gcagaagaag cccggcagca ggccatttcc ggcgggacgg aaccgtccgc ttttcctgat
      661 accctgccgg gttacaccga atatggccgg gacaacggca tccgtctgtc tgccgtgtgg
      721 ctgacgcacg acccggaata cccggagaat ttacctgccg cgccgctggt gcgctatggc
      781 tggacgccgc gcggcgaact ggcggtggtg tatgaccgta gtggcaaaca ggtgcgcagc
      841 tttacttacg atgataaata ccggggccgg atggtggcgc accgtcacac gggccggccg
      901 gaaatccgtt accgttacga cagcgacggg cgggtgacag aacagctaaa cccggcaggc
      961 ttaagctaca cgtatcagta tgagaaagac cgcatcacca tcaccgacag cctgaaccgc
     1021 cgtgaagtcc tgcacacgca gggtgaaggc gggctgaagc gggtggtgaa aaaggaacac
     1081 gcggacggca gcgtcacgca gagtcagttt gacgcggtgg gcaggctcag ggcacagacg
     1141 gatgccgcag gcaggacaac agaatacagc ccggatgtgg tgacgggcct catcacgcgc
     1201 atcaccacgc cggatggcag ggcatcggcg ttttactata accaccacag ccagttaacg
     1261 tcagccaccg ggcctgacgg gctggaaata cgccgggaat atgatgaatg gggccgtctg
     1321 attcaggaaa ctgcccctga cggcgatatc acccgctacc gttatgataa tccacacagt
     1381 gacttaccct gcgcaacgga agatgccacc ggcagccgga aaaccatgac gtggagccgt
     1441 tacggtcagt tgctgagctt caccgactgt tccggttatg taacccgtta tgaccatgac
     1501 cgcttcgggc agatgacggc ggtgcaccgc gaggaagggc tgagtcagta ccgcgcatac
     1561 gacagccgtg gacagttaat tgccgtgaaa gacacgcagg gccatgaaac gcggtatgaa
     1621 tacaacgccg ccggtgacct gaccaccgtc attgccccgg acggcagcag aaacgggaca
     1681 cagtacgatg cgtggggaaa agccatctgt accacgcagg gcggtctgac gcgcagtatg
     1741 gaatacgatg ctgccggacg ggtcatccgc ctgaccagtg aaaacggcag ccacaccacc
     1801 ttccgttacg atgtactcga ccggctgata caggaaaccg gctttgacgg ccgcacacag
     1861 cgttatcacc acgacctgac cggcaaactt atccgcagcg aggatgaggg gctggtcacc
     1921 cactggcact atgacgaagc agaccgcctc acgcaccgca ccgtgaaggg tgaaaccgca
     1981 gagcgctggc agtatgacga acgcggctgg ctgacagaca tcagccatat cagcgaaggg
     2041 caccgggtga cggtgcatta cgggtatgat gagaaaggcc ggctgaccgg tgagcgtcag
     2101 acggtgcatc acccgcagac ggaagcactg ctctggcagc atgagaccag acacgcttac
     2161 aacgcgcagg ggctggcgaa ccgctgtata ccggacagcc tgcccgccgt ggaatggctg
     2221 acctatggca gcggctggct ggcaggcatg aagctcggcg acacaccgct ggtggatttc
     2281 acccgcgacc gcctgcaccg gaaaacgctg cgcagattcg gccgttatga actcaccacc
     2341 gcttataccc ctgccgggca gttacagagc cagcacctga acagcctgca gtatgaccgc
     2401 gattacacct ggaacgacaa cggcgaactc atccgcatca gcagcccgcg ccagacccgg
     2461 agttacagct acagcgactc cggcaggctg accggcgttc acaccaccgc agcgaatctg
     2521 gatatccgca tcccgtatgc cacggacccg gcaggtaacc gcctgcccga cccggagctg
     2581 cacccggaca gcaccctcag catgtggccg gataaccgta tcgcccgtga cgcgcactat
     2641 ctttaccggt atgaccgtca cggcaggctg acagagaaaa ccgacctcat cccggaaggg
     2701 gttatccgca cggatgatga gcggactcac cggtaccatt acgacagtca gcaccggctg
     2761 gtgcactaca cgcggacaca atatgaagag ccgctggtcg aaagccgcta tctttacgac
     2821 ccgctgggcc gcagggtggc aaaacgggtg tggcgacgtg aacgggacct gacgggctgg
     2881 atgtcgctgt cacggaaacc gcaagtgacc tggtacggct gggacggcga ccggctgacc
     2941 acaatacaga acgacagaac ccgcatccag acgatttatc agccggggag cttcacgcca
     3001 ctcatcaggg ttgaaaccgc caccggtgag ctggcgaaaa cgcagcgccg cagcctggcg
     3061 gatgcgcttc agcagtccgg cggcgaagac ggtggcagtg tggtgttccc gccggtgctg
     3121 gtgcagatgc tcgaccggct ggaaagtgaa atcctggctg accgggtgag tgaggaaagc
     3181 cgccgctggc tggcatcgtg cggcctgact gtggcgcaga tgcaaagcca gatggacccg
     3241 gtatacacgc cggcgcgaaa aatccacctg taccactgcg accatcgcgg c
//
LOCUS       DAZFKY010000303         3190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFKY010000303 DAZFKY010000000
VERSION     DAZFKY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..1692
                     /locus_tag="ACHKCL_004719"
     CDS             172..1692
                     /locus_tag="ACHKCL_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5765520.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1717..2055)
                     /gene="maoP"
                     /locus_tag="ACHKCL_004720"
     CDS             complement(1717..2055)
                     /gene="maoP"
                     /locus_tag="ACHKCL_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5765521.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2174..3013
                     /gene="hdfR"
                     /locus_tag="ACHKCL_004721"
     CDS             2174..3013
                     /gene="hdfR"
                     /locus_tag="ACHKCL_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5765522.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3109..3184)
                     /locus_tag="ACHKCL_004722"
     tRNA            complement(3109..3184)
                     /locus_tag="ACHKCL_004722"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3149..3151),aa:Trp,seq:cca)
BASE COUNT          853 a          776 c          787 g          774 t
ORIGIN      
        1 cctccctcgt agttcatcct ttgtccgctg catttttgcg agcatcattc caccgccaga
       61 acaactcaac ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc
      121 gggctacagc agcgatatct tatgcacata atggtgactc aaggagggct tatgtcactg
      181 tcaattgttc atacccgcgc agccctggga gtaaatgcgc ccccaatcac tgttgaggta
      241 catatcagta aaggtttgcc cggcttaacg atggtgggct taccagaaac aacggtaaaa
      301 gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa
      361 aaaatcacca ttaaccttgc tccagccgat ctaccaaaag aagggggacg atatgattta
      421 cctatcgcca ttgcgttgct ggcggcctca gaacagctta cagccaataa gttagatgaa
      481 tatgaattag tcggagaact ggcgcttaca ggcgctctgc gtggcgttcc cggtgcaatc
      541 tccagtgcaa ctgaagctat taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa
      601 gatgaagtgg ggctaattaa cggtgaagga tgcctggtag ccgatcatct gcaagctgtc
      661 tgtgcgtttc tggaaggtaa gcacgctctc gaacgcccga aaccaactga tgcagtatcc
      721 cgggcgctac aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg
      781 gaaattaccg ccgctggcgg gcacaacctt ttactgattg ggccgccggg aacaggtaaa
      841 acaatgctcg ccagccgtat taatggtctt ttgccagatt taagcaatga agaggcactg
      901 gagagcgctg cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag
      961 cgcccgttcc gctcacctca tcacagtgcg tcgttaacgg cgatggtggg cggtggtgca
     1021 attccagggc caggtgaaat ttcgttggcg cataacggcg tgctttttct tgatgagcta
     1081 cctgaatttg aacggcgtac actggatgct ttgcgagagc cgattgaatc cgggcagatc
     1141 catctttcac gcacacgagc aaaaataacc tatccagccc gtttccagct tgttgcggcg
     1201 atgaatccca gccctaccgg acattatcag ggaaaccata accgctgcac gccagaacag
     1261 acattacgtt atctcaaccg gctctcgggg ccctttctcg accgcttcga tctctcactg
     1321 gagatcccat taccaccccc cggcattttg agtaaaacgg tagtgccggg agaaagcagc
     1381 accaccgtta aacaacgtgt aatggctgcc agagagcgcc aatttaagcg gcagaataag
     1441 ttgaacgcct ggctggatag tccggaaata cgcaaattct gcaagcttga gagcgaagat
     1501 gcgcagtggc tggaagaaac gctgatccat ctggggttat cgattcgtgc ctggcagcgg
     1561 ttattgaaag ttgcacgaac cattgctgat attgatcagt ctgacattat cacacgtcag
     1621 catttgcagg aggcagttag ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa
     1681 ctactgacat aaaaaaaggg catttcgccc tttttattaa tcgtcagaat cggtgtagtc
     1741 ttcagcacct tcaacctgcg gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt
     1801 gatacgcgtc atatacttgg accacacgcg ttctgcttct gtcactggct cacgttcgcc
     1861 acggcatact gctacgaaga gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc
     1921 caactcattg aaggcataac catgacgctc aagcagttgt gcctctttga tggtgaaatc
     1981 accatggcga gagaatccac gtggataatg tttattgtcg aaatatcgat tagtcgtcgt
     2041 aaagctttcc gccatcctgc acgctcctaa ttctttgacc gagctagtta tggcgcggag
     2101 tattagttac gcttgacaga gtgtaaaaca aaacatttaa atcataacga caaataattt
     2161 tgcggagagc actgtggata cggaattgtt aaaaactttc ctggaagtta gccgaacgcg
     2221 tcactttggt cgagcggctg aatcgctcta tctgacccag tcagcagtga gctttcgaat
     2281 cagacaactg gaaaatcaac tgggtgtgaa ccttttcacc cgccacagaa acaatatccg
     2341 tttaaccgct gccggtgaaa aactactgcc ttatgcagaa acgctcatga gcacctggca
     2401 ggccgcccgt aaggaggtgg cgcatacctc acgacataac gagttttcta tcggtgccag
     2461 cgcctcattg tgggaatgta tgcttaatca gtggctggga cgtttgtatc aaaatcagga
     2521 tgcccataca ggcttacagt tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca
     2581 gctgcatgaa cgccagcttg atcttcttat taccactgaa gcgcccaaaa tggacgaatt
     2641 tagtagtcag ttgctggggt atttcacttt agcgctttat accagtgccc cttctaaact
     2701 aaagggagat cttaactatc tgcgactgga gtgggggcca gattttcaac agcatgaggc
     2761 aggtttgatc ggtgctgacg aagtgcccat tctgacaacc agttctgctg aactggcaca
     2821 gcaacagatt gcgatgctta atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa
     2881 aggcggcctg cataccgttg tcgatagcac aacactttca cggccgcttt atgccatatg
     2941 gctgcaaaat agcgataaaa atacgttgat tcgcgatcta ttgaaaatta acgtgctgga
     3001 tgaagtgtat taatctgaat ggctggcaag gatgccggta gaaggattta cttcagagag
     3061 ggttatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gcaggggcgg
     3121 agagactcga actcccaaca cccggttttg gagaccggtg ctctaccaat tgaactacgc
     3181 ccctaattag
//
LOCUS       DAZFKY010000304         3188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFKY010000304 DAZFKY010000000
VERSION     DAZFKY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHKCL_004723"
     CDS             complement(<1..458)
                     /locus_tag="ACHKCL_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765523.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARA"
     gene            complement(478..939)
                     /locus_tag="ACHKCL_004724"
     CDS             complement(478..939)
                     /locus_tag="ACHKCL_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5765524.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(967..2868)
                     /locus_tag="ACHKCL_004725"
     CDS             complement(967..2868)
                     /locus_tag="ACHKCL_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5765525.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT          677 a          920 c          801 g          790 t
ORIGIN      
        1 gcacgggctg ctggctttcc gttaattttt acatttttag aaccggtatt gatggcgccg
       61 tccgtgtcac aaaagctgtt tatccatgat tcaagctggt tctgtttctc ctccacataa
      121 tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc ataagggccg
      181 gtggcacagg cggccataat caggcaacct tctgctatgg tcactatcgc ccccagcacg
      241 gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag cctggcggcg
      301 ggggggccag acttttttga tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt
      361 ttcatctggc tggcggccat cgcccgctgc gacagcatcc gcattgccag cgtaccgcct
      421 gcggtcgctc cctgtggccc gcctggtcct tcactcatgt tttatccctc gtttatgtca
      481 gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc tctttatcac
      541 ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga aagtgaaggt
      601 cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc cctgattccc
      661 ttcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct ctgccagagt
      721 gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt
      781 ttcctggtac agctctggtg tcagggttcc ctgtgaaacg ggttcgcggg taatgaccag
      841 gttgatgcca ctgttatccg tggataccag gacctgcatc gactgatcct gccattgcga
      901 tggcagttga atatgacctt cattaaaagt gaactgcata aaatactccc tgttaatctg
      961 agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct tcgacagcct
     1021 gattaccgtt gattccccga cctgtagctg aatgccatcc ggcgtcagcg caattttgct
     1081 ttcctccacg gccagcataa tttccttaat tgccgtataa gcctgtatac cgttgccttc
     1141 gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa
     1201 gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt tttccaccgt
     1261 aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg tttcgcgatg
     1321 atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc
     1381 gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat taaacccgct
     1441 gcccatatac gttttggaac ggatggtcat ctgcgtcttc gtccccggca ggctgccggg
     1501 ggtgcggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg gatcgccgtt
     1561 gaggaagtcc actatcacct cctgacccac acgcggtatc gccaggttgc caaaaccggt
     1621 gcctgcccac gcctgtgcca cacggatcca gcacgagctg tcctggtctg ccgggttata
     1681 acggtcccag ttgaatttca cccgcactcg gccatgttca tcacagaaga tttcctcacc
     1741 ctccggcccc gtcacgacgg cgctctgcgg accgtccacc agcggtttta gccgcggctg
     1801 gggtcgccat gtccggtctg ccgggatcac cgcaaaatgg ttctccagcg ccgtccctgc
     1861 tccccgcctt cccggcaccg cctgcggctg ctcgccgtgc agttcacttg ccaccacctg
     1921 ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgcc gtcccggcca
     1981 tatctccggc gagcgactca ttccccgcgc ggtttctgca ttgtttcgcc agccctccat
     2041 ctgccagcgg gcaaagttct gcccgtgggc gcccttgaaa cgtccggggt agtcatacac
     2101 ctcatactgc gtgcgctggt aatcctggtg ctggccttcc tgatcaaaac gtcctgccca
     2161 gccggggcgt ttaaaggtgt agtctttggt caccacggaa gaagggcgga tttgtgcgct
     2221 gtagcgaaac tggctgatgc agagggtgct cacctcggta cgggtgttcg ggttccaggg
     2281 gatttcaaaa gattcgggca gatggcggac cgtgtcgcac agcaccaggc tctggtcggt
     2341 acttttgtaa gcatgctcct cataaaagaa gatgccttcc tccgccgcca tccggcacag
     2401 gaaatcgtaa tcagtctcac cgtactggac acaaaactca cgggaaggat gcggttcact
     2461 gaacagcgga ctccactcgg tcaccccgtt ttcctgcaat atcgtgccga ggatgctttt
     2521 gatgtcctcg ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg
     2581 gtgcaccttc atgctgtaca gcatctggtt tttgtcgttc tcccccagtt caaaccaggt
     2641 caccacacct ttcacccggc gctgtacatc atcaccctgc catattgtca ggtaggccat
     2701 tttgtccagc acctgcgcaa attcaaggga gagaaactgc tggctgacca aggagagatc
     2761 gagggaaaag agcgaagaga gtgactgggt gagatgaaag gaaaccaccg caaaagcgtc
     2821 cggtggcagg ccgtccactt ccagtgtgaa acgtaatccg gttgacatag caacctccgg
     2881 taaaatgaat aataatccgc accgtacaac tgcggataat gagataagcg ggacgactta
     2941 agacgttatt agggaaggaa caaaaaatgc tatgattatg tgaggatttt gtttaaatat
     3001 tactttacag aaacgggtgt ctgttcagtc aaaaaaataa tcacaagctc aacttattgt
     3061 ttcctgacgt tattcaggac aggtgcgcac atcacagcga gtaacaaaaa cgcaaagtta
     3121 aaccaatatt cataatatgt ttttattcac aactaaataa cccaattaac atcattaaaa
     3181 tgaatgat
//
LOCUS       DAZFKY010000305         3186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFKY010000305 DAZFKY010000000
VERSION     DAZFKY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(29..655)
                     /gene="dsbA"
                     /locus_tag="ACHKCL_004726"
     CDS             complement(29..655)
                     /gene="dsbA"
                     /locus_tag="ACHKCL_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5765526.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(672..1658)
                     /gene="srkA"
                     /locus_tag="ACHKCL_004727"
     CDS             complement(672..1658)
                     /gene="srkA"
                     /locus_tag="ACHKCL_004727"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5765527.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1735..2004)
                     /locus_tag="ACHKCL_004728"
     CDS             complement(1735..2004)
                     /locus_tag="ACHKCL_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5765528.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2074..2658
                     /gene="mobA"
                     /locus_tag="ACHKCL_004729"
     CDS             2074..2658
                     /gene="mobA"
                     /locus_tag="ACHKCL_004729"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5765529.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2640..3167
                     /gene="mobB"
                     /locus_tag="ACHKCL_004730"
     CDS             2640..3167
                     /gene="mobB"
                     /locus_tag="ACHKCL_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5765530.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          833 a          772 c          767 g          814 t
ORIGIN      
        1 tcacgggctt tatgtaattt acattgaatt attttttctc ggacagatat ttcactgtat
       61 cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc ggattcagct
      121 gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacgtca gctgcagctt
      181 tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg tcgtactctt
      241 cacctttaat acctgcgttg ataaatacat cgcggatatc agatgctgaa cgaatggtct
      301 gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg cccagcgcca
      361 tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg aagttgacgt
      421 ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca gaaatatgca
      481 gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc agcacttgcg
      541 gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca tactgcgccg
      601 ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt ttcatgatct
      661 actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag aacttttgcc
      721 tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa ccacgggaaa
      781 tttttcggga acgcgggatc agcccaacgc cgcattagcc aggcaagata ataaaccaaa
      841 cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc gctaaattct
      901 tcataagctt caataatagt ttccagttgc atccgctgct cggctttatc gccattgagc
      961 aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc aacaaacatt
     1021 ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg cagaacggtg
     1081 aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc tttcaggaat
     1141 gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg
     1201 agatattcat tcagaccgat ggtcgggcga tggataaaaa gctgtttgcg ccccgtctgg
     1261 tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc agcttcgaac
     1321 tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt caataaagtc
     1381 tgaccgttaa aagccacagg tgctgcaacc ggaacttcat catttaccag ctgcaacgca
     1441 aattgatgtt cttcgaggat ttgatcggct gtccaacgtt caggacgata aaatttgacg
     1501 acaaaacgtc gacgatcttc gtcctgaaat tgatagacac ggttttcata gctgttaagc
     1561 ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat gatggtatcc
     1621 gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg aaaacattac
     1681 gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa gcttttactc
     1741 tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat aatctttctg
     1801 caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat agatcagaat
     1861 gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa
     1921 aaattgcaac aggttaaggt ctggctcttt ttgccaggct ggctggagga gttcaataac
     1981 ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag acacgttagc
     2041 agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac gataacaggc
     2101 gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg attgctggac
     2161 ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttatgacgca gctctctcac
     2221 gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct gaaagtgatt
     2281 gaagattcac tggcggatta cccaggccct ctggcaggga tgctttcagt aatgcagcag
     2341 gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc ccatgattta
     2401 gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa
     2461 cgcgatcacc cgactattgc tatggtaaac cgcgctattg agcctttatt actagaatat
     2521 ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg tcatgcggtt
     2581 gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga gcttgcccga
     2641 tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt accggaaaaa
     2701 ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt ccagggctga
     2761 ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc tatgagctgc
     2821 gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc ttgatgacgg
     2881 aaacaccaga cgaagaagag ctggatctac agtttctcgc aagtcgaatg gatacctcaa
     2941 agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag attgtgctgt
     3001 ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat gttattgctg
     3061 tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat gatgttgagg
     3121 ggctggctga ttttgtggtg gagtggatgc aaaatcagga cggataagca agagtaggct
     3181 ggagca
//
LOCUS       DAZFKY010000306         3184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFKY010000306 DAZFKY010000000
VERSION     DAZFKY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..299)
                     /locus_tag="ACHKCL_004731"
     CDS             complement(<1..299)
                     /locus_tag="ACHKCL_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765531.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLVLIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKREFRRKGELNQSNVFYLTLDNAQQIQPESGGA"
     gene            complement(462..761)
                     /locus_tag="ACHKCL_004732"
     CDS             complement(462..761)
                     /locus_tag="ACHKCL_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5765532.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSDQIQMEF"
     gene            complement(900..1127)
                     /locus_tag="ACHKCL_004733"
     CDS             complement(900..1127)
                     /locus_tag="ACHKCL_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765533.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            1206..1913
                     /locus_tag="ACHKCL_004734"
     CDS             1206..1913
                     /locus_tag="ACHKCL_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5765534.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1974..2315
                     /locus_tag="ACHKCL_004735"
     CDS             1974..2315
                     /locus_tag="ACHKCL_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HFO5765535.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRANPDCCFFG"
     gene            2383..2844
                     /locus_tag="ACHKCL_004736"
     CDS             2383..2844
                     /locus_tag="ACHKCL_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765536.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            2838..>3184
                     /locus_tag="ACHKCL_004737"
     CDS             2838..>3184
                     /locus_tag="ACHKCL_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO5765537.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIAMPFYHNGSLNQLMKKN
                     NLTSREIIRYSIQ"
BASE COUNT          931 a          622 c          700 g          931 t
ORIGIN      
        1 gccccacctg attctggttg gatttgttgt gcattatcca gcgtcagata aaaaacgttt
       61 gactggttaa gctctccttt tcttctgaat tcccttttca aaagccccat ctcttccagt
      121 gccctaatgt gactttttac tgtcgatctg ctcacctcac actggtcagc gacatgttga
      181 tatgaaggcc agcattcgcc attatcattg gcgttatcgg caagtttaat cagaaccagt
      241 tttctcagtg ggttgccaac ctttatattc atggccttag ccataagatt catgctcatt
      301 ttgacttctc cgaagttttg tacctgttaa gtatttctct cagtggcaca gctattgctg
      361 gattaacccc ctgataaaac tggtcacgta gcacatcttt tcggtgatta acgcgtttat
      421 tttcctgcgt ttttcgcata taattacctc gttggatgtt gttaaaattc catttgtatt
      481 tgatcagaac gctcggtctt gcacaccggg cgttttttat tggtgagtcc atcaagcgca
      541 tacttaaaag ccctgctaat cggactgatg tctgatgcca ttccgaaagc acacaagacc
      601 gaagcaataa acctccagtc cgttctgctt atcttcgatt catgacagcc aatcatcttt
      661 gccagaccac gctgggtaag cgttgacagg ttgatgagta aatcagtttc tgcgcgatca
      721 acgtcacgct gggatagttt gctgtaactt gtttgttcca tttcttaaga tttccaatag
      781 tgaatagtta gttgaaaggt atgcgtggaa acgcatatgg ccttagttgg tcagatatat
      841 tgggactcgc tttgtcagcg acgtaggacg aatgtccatt gtgaaaatag cggtgttact
      901 tatgcagcca gaaggttctt tttgcttatt tcaagcattt cgcttgcttg atatttgcca
      961 ccagaaatct cttcgatttt tgatgcgtat ttagttttcc caaaaaactc agtcttaggg
     1021 aggaagccgt ttttgagcca cttatagaca gccctttcgc taactccaca agccttcgca
     1081 acttcaggga tgccgacacc tttaatcggc tcatcaagat tttgcatagg aatatccttt
     1141 ttcgtacttt cagtacgtat tatggttgaa ctgaaagttt ttgcaagtgc tttagtatcg
     1201 tactcatggt tcagaatgaa aaagtgcgca aagaattcgc ccagcggcta gcgcaagcct
     1261 gtaaagaagc tggtcttgat gaacatggta ggggaatggc tatagcccgt gccctttctc
     1321 tttcgtccaa aggcgttagc aaatggttta atgctgagtc tttaccgcgt caggaaaaaa
     1381 tgaatgcgct tgcgaaattt ctaaacgttg atgttgtttg gcttcagcac ggcacttcgt
     1441 taaatggagc gaatgatgaa gatactcttt catttgttgg caaattaaaa aaagggttag
     1501 tgcgcgtggt tggtgaggca attcttggtg ttgatggtgc catcgagatg accgaagagc
     1561 gcgatgggtg gctcaaaatt tatagcgatg atccagatgc ctttggtctt cgtgtgaaag
     1621 gagacagcat gtggcccaga ataaaatcag gagaatatgt actcattgag cctaacacca
     1681 aagtattccc gggtgatgag gtgtttgtca gaaccgttga aggacacaac atgattaagg
     1741 ttcttggcta tgacagagat ggagaatacc aatttacaag cattaaccag gatcacaggc
     1801 ctataacgtt gccttatcat caagtagcaa aggtggagta tgtagctggt attctgaagc
     1861 aatctcgcca tctggatgac atcgaggcaa gggagtggct gaaaagttcg tgacttcatc
     1921 gtcacatagc tggtaaccag tggcctgaag agacgtttgg gtaaggagga tagatggcgt
     1981 tcaatgacct tgaatatcaa gcagtaaaaa aagaagttca ccaattcatt gaaagcataa
     2041 ggccgcctga acatatccgc aatgaactgg atattgttta tagcatcaat gaccaaacga
     2101 tagatatcgg cgaacagcgc cccgtgtggc agggcaatcc aggtgaaaca aacatcctgc
     2161 catcagcaag aatcaagtac atacgttctc tggatagatg gaaaatctat tggatgcgga
     2221 aggatatgaa atggcatcag tacagtactg aactttcgct gactgatgcg cttgagcttg
     2281 tgcgtgctaa cccggattgc tgcttcttcg gatgagtgaa gagacgtttg gatgatggat
     2341 ggtcgcagag atgcggcctg attctaaaat aggatataaa aaatgagaat actaggtgtt
     2401 agagcggcgc ccaaagttac atcttttgtt gtatattgca ctaatgagtc tgcactcaaa
     2461 tgtgttgatg ttattaaaat accttcgacc ttagacacac cagaaaaatt aaagtatgtg
     2521 agaaataaca tcctcgacat tcttaattta tataatgttg aattagctgc catacgcgtt
     2581 actgaatcaa actctgataa tcttagcatt gaccgccttt atatagaagc tgttattcaa
     2641 gaagcatttt caagcagtga tgtaagaaaa tattacacta ttagaaaatc tggcatgaaa
     2701 tcatcattga acctaacaga gatcgagtat aaagaaatat tgaagtcaca ccgcaatata
     2761 aatggaatcg ataattctgg ttttacaact gaaacaaatg aagctgtttt ggctgcacta
     2821 tctgcggagg taaggggatg ctaactccat acaaaagagc tgatgtagaa ttcgaatgga
     2881 ttagtgatct agaagaacag ggttgttttt caaaagtata tctggctcat gacagacacc
     2941 tagctcatga cttggtgatt aaagaaatag aaaaaaaaga aaacactaac cacgacgact
     3001 actttaatga agcaaggctt ctctataaac atgcacatcc aaatattgtg caagttcagt
     3061 atgctgctca atgtgagagc aatatctata tagccatgcc attttatcat aatggttcgc
     3121 taaaccaatt aatgaaaaaa aataatctta caagcaggga gataatacgg tattccattc
     3181 aatt
//
LOCUS       DAZFKY010000307         3169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFKY010000307 DAZFKY010000000
VERSION     DAZFKY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            47..841
                     /gene="hyxR"
                     /locus_tag="ACHKCL_004738"
                     /pseudo
     CDS             47..841
                     /gene="hyxR"
                     /locus_tag="ACHKCL_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            995..1756
                     /gene="hyxA"
                     /locus_tag="ACHKCL_004739"
     CDS             995..1756
                     /gene="hyxA"
                     /locus_tag="ACHKCL_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO5765538.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1798..2880
                     /locus_tag="ACHKCL_004740"
                     /pseudo
     CDS             1798..2880
                     /locus_tag="ACHKCL_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2870..3058
                     /locus_tag="ACHKCL_004741"
     CDS             2870..3058
                     /locus_tag="ACHKCL_004741"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5765539.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT          871 a          669 c          801 g          828 t
ORIGIN      
        1 tgaatcaaaa tgtcaggttt tgtagctatg gcgtggacga gtctgaatga agaagcctct
       61 ggttattatc agtgcctgtc agtttaccag gctggcactg gagtctctca tcccagcgga
      121 caggtatatc gtgcgggtgt acagcaacgt cactacagag gttgaattcg ttttaaagac
      181 ctcctgtggc tatctgttgg ctgatatccc ttagcttcct tcgggggagt tagttcttct
      241 ggcttgtctt gtccgcttgc ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga
      301 ctccgactgg ttcgataatg ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg
      361 caccatgctg acagcgagtc gttatcagaa tcaaattatc cgctggttac tacgtccttt
      421 gcccggcgat atgagcgatt tctggctcct gactgcacgg gagttgagcg tgcttaagat
      481 tctgatgcag ggcatgagtt tcagtgagat tgcccgttat gaacaacgca gttcaaagac
      541 cctgcatgcg attgccacac gggcattaat gaaacttgga ctgggaactc tgagcgattt
      601 caggctgctc tatacgggat gcggtgacag cagagtgaaa cggaatatac ggctacatgc
      661 aagatatgtc ggacaacatt ccgctcatcg actcactcag tatgtgcaga acatggtatc
      721 cggaattctt tatccagaga aagttgccgt tggtgaatca ataaattctg acgccatttc
      781 cggaaaagta cttcccggac aaagaaaaaa tatttctaaa aaaagaccag gattgttatg
      841 acacaacagc gttgttacta ccccccactg cggcgcgtac ggactcgcta cagtgtaggg
      901 gccaaaatat ttctactgct ctgggctgca ggattgggag gcacgctgta cagcctgtac
      961 agcgtgctgg cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt tctgtccctc
     1021 aggaattgta ccagcgcagt tcgcagctct atcaaccggt tctgtcggca atgttacctg
     1081 catcggaaca gaagaggagt tacgcaataa attgcggcac aggccacaga gtgtcgtgat
     1141 atcggcaggg cgacctgccg aatgcgctga aatgtggttc cgattttatc gggatcactc
     1201 attcgtggtg gtgttgtgcg tcgcgccatt ttttctgcca ccggatgtga gtatttctgg
     1261 cgttctcaaa aacctgaggt tactgaaacc aggaatgtca gtagagcatg tcatcagtat
     1321 tgcaaattcc tctggtgggt tatcagggct gaaacatgca gaaaatttgc cagttatgga
     1381 cagttactct gtctttatga aagaggtgaa taaccgcaca aagacaatcg tcatgtcaga
     1441 acgcttcccg gaaaagcaaa agaaggtgtt gtcgttgctt cttgccggac acagctggga
     1501 atacagtgcg cagtttctca aaacggggat aagacagata tggcttgccg agcagtcgct
     1561 aaaaaagcgt tggggaatac cggacagcat gtcattgcgg gaagccctgc tgctgagcag
     1621 caataaattt cacggtgatg gaaatgcatt ggaaaaaacg aatgccatga cattgcgtga
     1681 aaacggaaat accaacaggt attccgtggt ggtaaatgca gggacgcagg ttttatctct
     1741 gcataaatat aaataagtgg gaaattgaaa ttagttacca gaatggagaa tttcttcatg
     1801 aaaaacagta aggcatttta ccgcagcgca ttagcgacag ctattgttat ggctctttct
     1861 gcaccagcat tcgctgctga taacgcggta tcaactgatt cggttacgct gaataaagat
     1921 aagacgactc tggatcaaga tgttgttatt agcaatacag cagacaaaca gattacagcc
     1981 gtaacaatta atgcggcaga tgaagacctt aatgttgctt ttgctggtca tgatattact
     2041 gctgaatcaa cggcagacaa aaaattcgtt gaaggtgtaa aagttagtgg taacaaaaat
     2101 gttgtgatta atgctacaga ctccaccatc acagctcaag gtgaaggcac ctatgtccgg
     2161 actgcaatgg tcattgattc aactggcgat gttgttgtta atggcggtaa tttcgttgca
     2221 aaaaatgaaa aaggtagtgc gacagggata tctctggaag gggccaaggg aaataatgtc
     2281 acgcttaatg gcacaaccat aaatgctcaa ggtaataaga gttccagcaa cgcctccacg
     2341 gcaatttttg ctcaaaaggg tagcttattg aatagttttg aaggtgatgc aaccgacaac
     2401 attacccttg ctggctcaaa tattattaat ggtcggattg aaacaatagt tactgccggg
     2461 aataagacgg gaattcatac agtcaacctg aatattaagg atggctcagt aattggggcg
     2521 gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcacagg ttcagcaacg
     2581 caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct
     2641 gaaagcgaga attcagccgc taccacaaca aatgtaaaca tgaacgttgc ccgctcttac
     2701 tgggaaggta atgcttatac tttcaatagc ggcgataaag cgggtagtaa tctggatata
     2761 aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaaa tgccagtgta
     2821 tctctgcaaa acgagtctgt ctggaatgtt acgggatcct caactgttga tgctctggct
     2881 ccgtctgggt gatgagtctg tcaatgccag cattgacggt gcagcattcc gcgtgggtgc
     2941 aggtgtacag gctgatatca ccaaaaacat gggagcatat gcaagccttg actacaccaa
     3001 aggtgacgac attgagaatc cgctacaggg tgtagttggt atcaatgtga cctggtaaga
     3061 ataaagcagt cggttcccct gtcgactgtt ataccccctg caggcagcag tatgctgcct
     3121 gcaggctata aatatccagt attcatgatg ctggcttttt gctgatatt
//
LOCUS       DAZFKY010000308         3153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFKY010000308 DAZFKY010000000
VERSION     DAZFKY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            115..591
                     /gene="agaB"
                     /locus_tag="ACHKCL_004742"
     CDS             115..591
                     /gene="agaB"
                     /locus_tag="ACHKCL_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5765540.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            663..1433
                     /gene="agaC"
                     /locus_tag="ACHKCL_004743"
     CDS             663..1433
                     /gene="agaC"
                     /locus_tag="ACHKCL_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5765541.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1423..2214
                     /gene="agaD"
                     /locus_tag="ACHKCL_004744"
     CDS             1423..2214
                     /gene="agaD"
                     /locus_tag="ACHKCL_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5765542.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2215..2970
                     /gene="agaI"
                     /locus_tag="ACHKCL_004745"
                     /pseudo
     CDS             2215..2970
                     /gene="agaI"
                     /locus_tag="ACHKCL_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT          807 a          720 c          763 g          863 t
ORIGIN      
        1 gtgccttaat ggaaaaggag atgactaaac cttaataaat agatcagtac aatatcgcaa
       61 caataatata tttaaaaaaa ttatattatt caactttatg gtgaggatta cacaatgagc
      121 agtccaaata ttctcttaac ccgtattgat aaccgtctgg ttcacggtca ggttggcgtg
      181 acctggacat ccaccatcgg tgcgaatctg ctggtagtcg tggatgatgt tgtcgctaac
      241 gatgatattc aacagaaatt aatgggtatt accgcagaaa cctacggctt tggcattcgt
      301 ttctttacta tcgaaaaaac cattaacgtc atcggcaaag ctgcaccgca tcagaagatc
      361 ttcctgattt gccgtacgcc acaaacggta cgtaaattgg tagaaggtgg cattgacctg
      421 aaagatgtca acgtcggcaa tatgcatttc tcggaaggga aaaagcaaat cagcagtaaa
      481 gtttatgtcg atgaccagga tctcacggac ttacgtttta ttaaacaacg tggcgtgaat
      541 gttttcattc aggacgtccc tggcgatcaa aaagaacaaa tccctgacta aatctaaaat
      601 cgccttaata ttggtttgag gtaataaaaa tgcatgaaat aaccctactt tagggattat
      661 ccctggcggc gttagttttc gttctgggga ttgatttttg gctggaagcc ttatttttat
      721 tccgcccgat aatcgtttgt accctaactg gcgctattct cggtgatatt cagactggct
      781 taattaccgg tggtctgaca gagttggctt tcgccggatt aacccctgca ggtggtgttc
      841 agccgcccaa cccgattatg gcgggtctga tgaccaccgt cattgcatgg tctacgggcg
      901 ttgatgccaa aacggcaatt ggtcttggcc tgccgtttag tttgttaatg cagtacgtca
      961 ttctgttctt ctattctgct ttctcattat ttatgaccaa agccgataaa tgcgcgaaag
     1021 aggcggatac ggcagcgttt tcccgactta actggacaac gatgctcatc gtcgcttcag
     1081 cgtatgcggt gattgctttc ctctgtactt acctggcaca aggggcgatg caggcgctgg
     1141 tgaaagcgat gcccgcttgg ctgacccacg gctttgaagt ggctggcggt attctgcctg
     1201 ccgttggttt tggcttgctg ctgcgcgtga tgttcaaagc acaatatatc ccttacctga
     1261 tcgccggctt cctgtttgtt tgctacatcc aggtcagcaa cctgttgccg gttgccgtac
     1321 tgggcgcagg ctttgcggtg tatgagtttt tcaatgcgaa atcccggcag caagcgcaac
     1381 cgcagcccgt tgccagtaaa aatgaagaag aggactacag caatgggatc tgaaatcagt
     1441 aaaaaagata tcacccgtct gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac
     1501 gaaaggatgc aagcgggcgg ttttacctgg gcgatgttgc cgatcctgaa aaagatttat
     1561 aaggatgaca aaccgggcct aagcgcagcg atgaaagata acctcgaatt tattaatacc
     1621 cacccgaatc tggtcggatt cctgatgggg ttattaattt cgatggaaga aaaaggagaa
     1681 aaccgcgaca ccattaaagg cctcaaagtg gcactgtttg gcccaatcgc cgggattggc
     1741 gatgcgattt tctggtttac tttgttgccg attatggcgg gaatttgctc atcatttgcc
     1801 agccagggaa acctgctggg gccgattcta tttttcgccg tttacctgct tatctttttc
     1861 ctgcgcgtcg gctggaccca cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg
     1921 cgagagaact cgcagatgat tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc
     1981 gggctgatcg cttcgtatgt gcatattaac gtggtgacat cgtttgccat cgacagtacc
     2041 cacagcgtcg cgctgcaaca ggatttcttc gataaagtct tcccgaacat tttaccgatg
     2101 gcctacaccc tgctgatgta ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta
     2161 atcggcgtga cttttgtgct ctctattgtt tgttccgcat tcggcatttt gtaaatggaa
     2221 cgaggcactg cgtctggcgg tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt
     2281 cagcaagttg aaaactatac ggcgttaagt gaacgtgcca gcgaatattt attggccgtg
     2341 atccgtagca aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc attactgacg
     2401 tatcattatc tggtagaaaa aatccactag cagcaggttg atgtcagcca gctcaccttc
     2461 gtgaagctcg acgaatgggt ggatctgcca ttaacgatgc ctggtacctg cgaaactttc
     2521 ctgcaacagc atatcgtgca gccgctgggg ctacgtgaag accagctcat cagctttcgc
     2581 tccgaagaga taaatgagac agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc
     2641 ggtctggatt tatgcgttct cggattgggg aaaaacggtc atcttgggct gaacgaaccg
     2701 ggagaaaacc tgcaaccggc ctgccatatc agtcaacttg atgccagaac acagcaacat
     2761 gagatgttaa aaaccgcggg tcgccccgtg actcgtggga tcaccttagg cctgaaggat
     2821 attctcaatg cccgcgaggt tttgttactg gtgactggcg aaggaaagca ggatgcgaca
     2881 gaacgttttc tcacggctaa agtctctacc tctatcccgg cttcgttttt gtggctgcac
     2941 agtaacttta tttgtttaat taatacctaa ccagatgaaa aatttgctca aaatgtttaa
     3001 attaacttat gcaacagtca cgcattatat taaataacat ttgactgggt tgaacataac
     3061 gccaatagca aaaggagtta tgttcaacaa tttaatttta gatatacata taacttgttc
     3121 tattaataaa ccatctcgta ttctgactcg ccc
//
LOCUS       DAZFKY010000309         3137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFKY010000309 DAZFKY010000000
VERSION     DAZFKY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            10..1053
                     /locus_tag="ACHKCL_004746"
     CDS             10..1053
                     /locus_tag="ACHKCL_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5765543.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            1046..1507
                     /locus_tag="ACHKCL_004747"
     CDS             1046..1507
                     /locus_tag="ACHKCL_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765544.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            1541..2257
                     /locus_tag="ACHKCL_004748"
     CDS             1541..2257
                     /locus_tag="ACHKCL_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5765545.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2290..2571
                     /locus_tag="ACHKCL_004749"
     CDS             2290..2571
                     /locus_tag="ACHKCL_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5765546.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2568..2795
                     /locus_tag="ACHKCL_004750"
     CDS             2568..2795
                     /locus_tag="ACHKCL_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765547.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2788..3099
                     /locus_tag="ACHKCL_004751"
     CDS             2788..3099
                     /locus_tag="ACHKCL_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765548.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          839 a          676 c          978 g          644 t
ORIGIN      
        1 gaggtgagta tggctaatgc ctggctcaga ttgtggcatg acatgccaaa tgaccccaag
       61 tggcgaacga ttgccagggt atcaggacag ccaatcgcaa cagtgatggc agtgtatatc
      121 catcttctgg tgagcgcgtc acgaaatgtc acgacatgtc acggcgtgtc actacgtggt
      181 cacattgatg tcacgacgga agatttagca agtgcgcttg atgtgacgga agacgtaatt
      241 gattcaattt tgcatgcaat gcaggggcgg gttctggatg gtgaccttat ttccggatgg
      301 gaaaaacgtc aggtgctgaa agaggacaat ggtaacgttt cgcaaacggc aaaatccccg
      361 gcagagcgca agagagcgca gcgggagcgg gaaaagctgc ggaaacataa tgctgattgt
      421 cacgatgagt cacgacgtgt cacgcatctg tcacgacaag tcacgacaga taaagataca
      481 gataaagata cagatacaga attaaacccc acacataacg cgcgcgagag tattccgacc
      541 agtgagtcga atggtgcgcc gttgcagaca gccgaacctg aatacctgga cggcctgagc
      601 gaaccgatcg ggaaattttc gatgactact gtctggcagc cgtcgccgga ttttcgacaa
      661 cgggcagcag tgtggggtat ggctctgcct gagccggaat ttacacctgc tgagcttgcc
      721 gcattccggg attactggat ggcggagggg aaggttttca cgcaggttca gtgggagcag
      781 aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa aaccagtcag caagggggga
      841 agccatgcag catcaggtgg cacggcatca cgggcagttc aggaaatccg ggctgcacgc
      901 gaacagtggg aacgtgacaa cggatttatc agcaacggaa acggcctgga agctgtggga
      961 gctcatgggg gaggtgtatt cgaaccgctg gactcagaag aacggggccg caccttcgaa
     1021 gctctggatt gcccagattg gtgcgatgac tgaacagcaa atccgtctgg tgtgccgtca
     1081 gtgcatggac cgctgccggg cgggtgaaac gtggcccccg gacctggctg agtttgttgc
     1141 gctgatttcg gagagtgggg caaatccatt tggtcttacg gtggatgccg tgatggaaga
     1201 gtaccggcgc tggcgcaatg aatcctggcg atacgacggg agtgataaat acccgtggcc
     1261 acagcctgtg ctgtaccaca tctgcctcga aatgcgtacc agagggattg agcgccagat
     1321 gacgcaaggt gagttaaaac gacttgcgga acggcaactg acgaaatggg caaagcatgt
     1381 tggtaacggg atgagtgttc cgccagtgcg acgacaactg gaaggggcga aacacccgca
     1441 agggccaacg ccaattgaac ggctgaaaca ggaatacgaa cgccggaagg cagctggttt
     1501 tatttgaatc tgagaaacga ttttgtcgga ggaaatatta atggaaaccg tatttgacgc
     1561 actgaaagca ctgaaaaaag cctcttcaca ggtagtggca tcgcgccttg gaatcagccg
     1621 cgaagatgct gtcaacgaac tgtggaaact gaagcgccgt ggtgaagcgg ataacaaggg
     1681 gtcgatgtgg tggctgactc agactggtga aagtgaacca gtgtcaccgg taccgaaagt
     1741 gacagcgcaa atgctgactg aggcgattga acatcatggc ccacaaacgg cggatgagct
     1801 ggcactgatg ttcgggatta cctcccgccg ggcgaattca tcactggcca tggcaatcag
     1861 caaagggcgt ctgattcgcg tgaatcaggg cggtaaattt cggtactgca taccgggcgc
     1921 tgatttaccg gcagagccga aagccgcatc cataacggaa acggatggta aagcctttcc
     1981 tcagccagca ggtgttgcgt taccagtcgg ggaagcggaa acacaggaag aaataaaaac
     2041 ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc accagaaagc atccggatgg
     2101 tctgatttta ccatcgctgc atgtggctaa ccgcgagctg cgccgggcaa aaggtcaggt
     2161 tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagtgcc gggatattct
     2221 ccgggatatt accgccacca gagaacagca gcggtgagtg ggtggaagac gtggtgccgg
     2281 gcggaaatca tgatactccg gcagtgtgcg ggaacgatga aggtaaaaag cgttggcgca
     2341 cttatcggac gaactgaagc ggcagtgaga acgaaggcac gggagctggg catcagcatg
     2401 atgttacgtg gtgattttca cccgtcggca aaatattctc agcgtgatat tgagctggcg
     2461 cggcaactgc atcagagagg catgcaaaga agggaaattg ccagaaaatt aggcatgccg
     2521 ctgcgcatag tgaataacta cgtttatttc gacaggaggg tgtctgcgtg aaaatcctgt
     2581 atcaggatta cggcccggtg gggcaggtgg ttatcagcag tactgtaatg gagtttcgga
     2641 agcataaccg tgtggtggat gctgtgctgt taacctgtcc ggggatatcg gcgagtcgtg
     2701 caggtgtgtt tattatgaag acgaaattat atggcagtaa ggcgtggata aagaaggcgt
     2761 atcgtgtagc gttgcaggag gttaacagtg agtgaaatta aagaaatgcc ggtagttcgt
     2821 gacggatatg gctactggac acatcctgaa tatgaaaaat tctgtgatgg tcgggaatat
     2881 atttcaacgg aagagtttaa cgcctggatg gaggaaaata atcttcaata cgtcctctgc
     2941 ttcagagatg aaggatgtgc tgaccttgat gcgtgtgatg ctgatatttc tgcatgggaa
     3001 ccggaacgac cagagggcga tggctggttt attggttcca ttcatgatac ggaagatggc
     3061 ccggtttgtg tctggttgcg aaataaggct gaagcataaa ggcgataaac caactaacaa
     3121 ctaaatactg aagattt
//
LOCUS       DAZFKY010000310         3109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFKY010000310 DAZFKY010000000
VERSION     DAZFKY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1683
                     /gene="ydeN"
                     /locus_tag="ACHKCL_004752"
     CDS             <1..1683
                     /gene="ydeN"
                     /locus_tag="ACHKCL_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5765549.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1735..2892
                     /gene="ydeM"
                     /locus_tag="ACHKCL_004753"
     CDS             1735..2892
                     /gene="ydeM"
                     /locus_tag="ACHKCL_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5765550.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2858..2980)
                     /locus_tag="ACHKCL_004754"
     CDS             complement(2858..2980)
                     /locus_tag="ACHKCL_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765551.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          942 a          674 c          633 g          860 t
ORIGIN      
        1 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
       61 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
      121 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
      181 atcgtactga ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
      241 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
      301 gccattgaag ctgcgcaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
      361 cgttttacta acggctatgt ggcgcacggt gtttccggcc cctcccgcgc cgcaataatg
      421 accggtcgag cgcccgcccg ctttggtgtc tattccaata ctgatgctca ggatggtatt
      481 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
      541 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaaca
      601 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc gcaaaaccgt
      661 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
      721 ctgttcaaga atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
      781 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
      841 gcttataatg ctccgcacct gccaaatgat aatcctgcac cggagcaata tcagaagcaa
      901 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
      961 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     1021 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     1081 aaaggctata agagtcagac ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg
     1141 aaaggaaaac ttcaacccgg taattatgac aagctgattt ccgcaatgga tttctacccg
     1201 acagctcttg atgcagccga tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc
     1261 ttgctgccct ggttgcaaga taagaaacaa ggcgagccac ataaaaatct gacctggata
     1321 acctcttatt ctcactggtt tgatgaggaa aatattccat tctgggataa ctaccacaaa
     1381 tttgtccgtc atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa
     1441 ttctcttata cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag
     1501 ttaggtctct acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg
     1561 caggtcgtta aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg
     1621 cttagcgagg taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa
     1681 taactaaacc ttcatgcggc ggatttttcc gccgccttat tgagcgagat agcgatgcac
     1741 gttacagcca agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt
     1801 gaaaaagagt cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc
     1861 atcaaacaat atatcgcagc gtctggcaat caggtctatt ttacctggca aggcggtgaa
     1921 cccactctgg ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca
     1981 ggccaaaaac gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg
     2041 tgtgcctttc tcaaagaaca tgagtttctg gttggtatct cgatcgatgg accccaggag
     2101 ctacatgacc gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca
     2161 gccatcgagc gtctgaaatc atatcaagta gagtttaata cgttaaccgt cattaataac
     2221 gttaatgtcc attaccctct tgaggtttat cattttttaa aatctatcgg cagtaaacat
     2281 atgcaattta tcgaattgct cgaaaccggg acgccgaata ttgatttcag tggtcatagt
     2341 gagaacacat tccgtatcat tgatttttct gtgcctccca cggcttatgg caagtttatg
     2401 tcaaccattt ttatgcaatg ggttaaaaac gatgtgggtg aaattttcat ccgtcagttt
     2461 gaaagctttg tcagccgttt tttggggaat gggcatacca gttgtatttt ccaggagtcc
     2521 tgcaaggata atctggttgt tgaaagtaat ggagatattt acgaatgcga tcattttgtc
     2581 tatccacagt acaaacttgg aaacattaat aaatctgaac ttaaaacgat gaacagtgta
     2641 caactgacag cgcaaaaaaa acggatttca gcgaaatgtc agcaatgtgc atataaacct
     2701 atctgcaatg gcggttgccc taagcatcgt attactaaag taaacgatga gactgtttcc
     2761 tatttttgcg aaggttataa aatccttttt tcaaccatgg taccttatat gaacgccatg
     2821 gtggagttag ccaagaacag agtaccgctt taccacatta tggatgttgc aaaacaaatg
     2881 gagaataatt aataacgctc tcttttagct ccattcttag cctccttctt atgaccttaa
     2941 caaggcaccc gacataaatt cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa
     3001 atcttggctt acgtggtatg cgtcagggca ttagcaatac ccttgattat caatagcata
     3061 accataacca ctgaaaatta aaaatttcta tatatttcat attgttaaa
//
LOCUS       DAZFKY010000311         3044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFKY010000311 DAZFKY010000000
VERSION     DAZFKY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            110..>3044
                     /gene="ehaB"
                     /locus_tag="ACHKCL_004755"
     CDS             110..>3044
                     /gene="ehaB"
                     /locus_tag="ACHKCL_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO5765552.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKY"
BASE COUNT          830 a          707 c          706 g          801 t
ORIGIN      
        1 gaaccattcc tctataacgc cgcataatcg tcaacgcgaa acataagatg ttttctatgc
       61 attgataatt gatggatcaa cttattacgt ccctgaggag ggatgacaaa tgcactcctg
      121 gaaaaagaaa cttatagtat cacaattagc attggcttgc actctggcta tcacctctca
      181 ggctaatgca gcgaccaacg atatttctgg tcaaacttac aatactttcc atcactacaa
      241 cgacgccacc tatgctgatg acgtttacta tgatggttat gtaggctgga acaactatgc
      301 cgctgatagc tattacaacg gcgatatcta cccggtcatt aataacgcta ccgttaacgg
      361 cgtgatttct acctactatc tggacgacgg tatttctacc aataccaacg ccaatagtct
      421 gacaatcaaa aacagcacta ttcacggtat gattacctct gagtgcatga ctactgattg
      481 tgctgatgac cgtgctactg gttatgttta tgatcgtctg acactgagcg ttgataattc
      541 aacgatcgat gacaactacg agcattatac ttacaacggt acctataata atgccgctga
      601 cactcatgtt gtagatgttt acgatatggg tactgctatt acactggatc aggaagttga
      661 tctgtccatc actaataact ctcatgtagc aggtattacg ctgactcagg gttatgagtg
      721 ggaagatatt gacgacaaca cagtcagcac tggcgtaaac agcagcgaag tgtttaataa
      781 cactattact gttaaagatt ctactgtgac ctctggttca tggactgatg aaggtactac
      841 tggttggttt ggccatactg gtaatgccag caactatagc aacacgctga ctgcagacga
      901 tgttgcaatt gccgcaatcg caaatccgta tgctgataat gcgatgcaga ctacagtaac
      961 tttagacaac tcaacactga tgggtgatgt tgttttctcc agtaatttcg atgaaaactt
     1021 cttcccgcaa ggtgctaaca gctatcgcga tgctgatggt gatgtagata ccaacggttg
     1081 ggatggcaca gaccgtatgg atgtgactct gaacaacggc agcaagtggg ttggcgctgc
     1141 aatgtctgtt catatggttg atgaagatgg tgatggttct tacgacggat atgctgttgg
     1201 tactgaagca actgcaactc tgctcgatat tgcagctaac agcctgtggc cttcatcaac
     1261 tgtcggtgtt gataacatca atactcaata tgacgaaaat ggccatatcg taggaaacga
     1321 agtttaccag agcggtttgt ttaatgtgac tttgaacggt ggttcagagt gggatacaac
     1381 aaaatcttct ctgattgata ctttaagtat taacagcggt tcccaagtta atgttgcaga
     1441 ctctcgtctg atctctgaca ctgtctctct gactggcggt tctaacctga acatcggtga
     1501 agacggtcat gtagcgacta ataccctgac catcgacaat agtaccgtta aaatgtctga
     1561 tgatgtttct gcgggctggg gtttagaaga tgctgcactg tacgcaaata ccatcaccgt
     1621 aactaacgac ggtctgttgg atattaacgt tgatcagttc gatgctaacc cgttccaggc
     1681 cgataccctg aatctgacca gtaccactga tactaacggc aacattcacg ctggtgtatt
     1741 cgatatccat agcagtgatt acgtaatgga taccgatctg gtcaacgatc gtaccaacga
     1801 tactaccaag tcaaactacg gttatggctt aatcgcaatg aactctgatg gtcacctgac
     1861 tattaacggt aacggcgata acgacaacac tgcttctatc gaagctggtc agaacgaagt
     1921 tgataacaac ggtgaccatg ttgcagccgc gaccggtaac tacaaagttc gtatcgacaa
     1981 cgctactggt gctggttcta tcgctgacta caacggcaac gagctgatct acgtcaacga
     2041 caaaaacagc aacgcgacct tctctgctgc taacaaagct gacctgggtg catacaccta
     2101 tcaggctgaa cagcgcggta acaccgttgt tctgcaacag atggagttga ccgactacgc
     2161 taacatggcg ctgagcatcc catctgcgaa caccaatatc tggaacctgg aacaagacac
     2221 cgttggtact cgtttgacca actctcgtca tggcctggct gataacggcg gcgcatgggt
     2281 aagctacttc ggtggtaact tcaacggcga caacggcacc atcaactatg atcaggatgt
     2341 taacggcatc atggtcggtg ttgataccaa aattgacggt aacaacgcta agtggatcgt
     2401 cggtgcggct gcaggcttcg ctaaaggtga catgaatgac cgttctggtc aggtggatca
     2461 agacagccag actgcctaca tctactcttc tgctcacttc gcgaacaacg tctttgttga
     2521 tggtagcttg agctactctc acttcaacaa cgacctgtct gcaaccatga gcaacggtac
     2581 ttacgttgac ggtagcacca actccgacgc ttggggcttc ggtttgaaag ccggttacga
     2641 cttcaaactg ggtgatgctg gttacgtgac tccttacggc agcatttctg gtctgttcca
     2701 gtctggtgat gactaccagc tgagcaacga catgaaagtt gacggtcagt cttacgacag
     2761 catgcgttat gaactgggtg tagatgcagg ttataccttc acctacagcg aagatcaggc
     2821 tctgactccg tacttcaaac tggcttacgt ctacgacgac tctaacaacg ataacgatgt
     2881 gaacggtgat tccatcgata acggtactga agggtctgcg gtacgtgttg gtctgggtac
     2941 tcagttcagc ttcaccaaga acttcagcgc ctataccgat gctaactacc tcggtggtgg
     3001 tgacgtagat caagactggt ccgcgaacgt gggtgttaaa tata
//
LOCUS       DAZFKY010000312         2928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFKY010000312 DAZFKY010000000
VERSION     DAZFKY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..607
                     /locus_tag="ACHKCL_004756"
     CDS             <1..607
                     /locus_tag="ACHKCL_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5765553.1"
                     /translation="VSFLSDIPQETLSEIRNQTIRGEAQVRLGELMVSIRPMQVNGYF
                     MGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEMLENMELLSNYQL
                     EELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMNSNVCMLYDKMSL
                     IYLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            complement(1188..1622)
                     /gene="uspF"
                     /locus_tag="ACHKCL_004757"
     CDS             complement(1188..1622)
                     /gene="uspF"
                     /locus_tag="ACHKCL_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5765554.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1763..2876)
                     /gene="ompN"
                     /locus_tag="ACHKCL_004758"
                     /pseudo
     CDS             complement(1763..2876)
                     /gene="ompN"
                     /locus_tag="ACHKCL_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
BASE COUNT          829 a          584 c          679 g          836 t
ORIGIN      
        1 ggttagtttt ttgtctgaca ttcctcaaga aaccttgtct gagatacgta atcagactat
       61 tcgtggagaa gctcaagtaa gactgggtga gttgatggtg tcaatacgac ctatgcaggt
      121 aaatggatat tttatgggaa gtcttaacca ggatggttta tcgaatgata acatccagat
      181 tggccttcaa tatatagaac atattgaacg tacacttaat catggtagtt tgacaagccg
      241 tgaagttaca gtactgcgtg aaattgagat gctcgaaaat atggaattgc tttctaacta
      301 ccagttagag gagttgttag ataaaattga agtatgtgca tttaatgtgg agcatgcaca
      361 attgcaagtg ccagagagct tacgaacatg ccctgttaca ttatgtgaac cagaagatgg
      421 ggtatttatg aggaattcaa tgaattcaaa tgtttgtatg ttgtatgata aaatgtcatt
      481 aatatatctt gttaaaacaa gggcggctca tcctttgagc agggaatcaa tcgcagtttc
      541 aatgattgta ggaagagata attgtgcttt tgaccctgac agaggtaact tcgttttaaa
      601 aaattaaggt agcaatggcg ggttaacttt tcgtaaaatg aaattaattc tcggtacggc
      661 atgtaaaacg cggcacctcg tatgcaagaa cgtgccgcgg ttggctggta agcttttcga
      721 tagtgcgagt atctaatgat ttccagccgg aactgattct attggaaaga aatcatgggc
      781 gccagattga ttagttatag atttttactg tggcaaaaat gggacaaaag gccgcaaaag
      841 gggcaaaaat ggggcaaaaa aagtgtagat tattgtgctt tattgtagtt gttaatcctg
      901 cctaacatat tgaaaaacat aaaagttatt gttcgtcaga tagttgcggt ttttactctg
      961 acttgttcag gttgtattgt tctttcttgc taatttcttg attttgcgac atttaaaagc
     1021 gactcaattc gttatatggc atcagaagag tatgcgtcat gccggaacgc ccagcataag
     1081 aaatctgata taaaaactgt ggcgtgtatg gtacggatta gaggggaaaa tgtcagcaca
     1141 tttgcgaaat gaatcaaaaa gcccgcagca atgtgcgggc gttagtgtca gcgcacaacc
     1201 agcacggagc actctgcgtg acgcactaca gctgcggcgt tggaaccgag cagataagtg
     1261 gtgatatccg gtcgatggga agcaatgatg atcatatgag cggggatctt cttcgccaat
     1321 tccagaatgc ggtctttggg cgagccttcc tcaacatgga catgcactct gtcggttggc
     1381 agtttaaatt ttttaatgat ctcttccagt tgcgatttgg cttccgcttt caggtcatcc
     1441 attgccggta attctgcgga atacgctaaa cccagagagg catagtaggg cagtgaaggt
     1501 attaccgtca ggaaatgaac ctctgcatca tcaatctttg cctctgcctc aacgtggcta
     1561 atcacgcgtt gagttaattc tgaatcggaa atatcgatag ggacaagaat cgttctgttc
     1621 ataaaacctc ctgttttagt atccgcataa agtgtaacgc cagatgacac tttttgtgta
     1681 atgacggagt tcacattttt aatttagatc aaaggaggaa gaataagcag aaaaagcccg
     1741 ccataacagc gggcaggagg atttagaact gataaaccag acctaaagcg acaatatcat
     1801 cggtagagat gccgttggca gcgtagaaac tgtcatcttc atccaacagg ttgattttat
     1861 agtcaacata agtggacatg tttttattga aatagtaagt cgcgccaaca tcggcgtatt
     1921 taaccagatc tttatcatca acacctgccg ggttgtctgc accaccggca gcgtgcaggt
     1981 cacggccttt agacatcagg aaagagactg ccggacgcag accaaaatca aactggtact
     2041 gtgcagtgac ttcaaagttc tgggttttgt ttgcaacagc ataatcgctg tcgccaaacg
     2101 gggtcatatt acgcgtttct gaatacatgg ttgccaggta aatattgtta gcatcgtatt
     2161 ttagcccagc agtccacgcg tctgctttat caccacccgc cgcagtatgg ttaacctggt
     2221 cattggtgcg gtcagaagag gtgtatgccg caccagcgct aaagcccatg cctaaatcat
     2281 atgttgtgga aagaccccag ccgtcaccgt tttcatggcg aacatcacgt ccgttgttgg
     2341 tgccttcctg accattactg gcaccttcgt tgttaccttg atactgcacc gcgaagttca
     2401 gaccatttac cagaccgaag aaatcagtat tacgataagt cgcgacgcca ttggcacgac
     2461 cagtcataaa gttgtctgca ttggtataag agtcaccgcc aaattcaggc agcatatcgg
     2521 tccagcccat aattacgtcc gtaatcgaaa gaaccgtaat ctgaaaattt cagtccggca
     2581 aatgccagac gggtccatga ctggtttttt gaagattcag tgttgtttgc ctgaatattg
     2641 tattcccatt gaccgtagcc agtaagttga tcgttaattt gggtttcgcc tttaaaaccc
     2701 agacgcgcat agctctggtc gccatctttc gctgaattat cagaaaaata atgcaggcca
     2761 tcaactttgc catacagatc taatttgttg ccgtctttat tataaacttc ggctgcatgt
     2821 gcagcacctg cgccgagcag ggcaggaatt aaaagtgcca gtactttgct tttcattgaa
     2881 taaatccttt agttatttta tttgcctttt atccccattt cggggagt
//
LOCUS       DAZFKY010000313         2926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFKY010000313 DAZFKY010000000
VERSION     DAZFKY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..164
                     /locus_tag="ACHKCL_004759"
     CDS             <1..164
                     /locus_tag="ACHKCL_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5765555.1"
                     /translation="DTCIGKRLHKAEGIVEGMLMMLGVRLEMDRYVERELPGGRTSVF
                     YQRKNSLRS"
     gene            671..1618
                     /gene="stxA1a"
                     /locus_tag="ACHKCL_004760"
     CDS             671..1618
                     /gene="stxA1a"
                     /locus_tag="ACHKCL_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5765556.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1628..1897
                     /gene="stxB1a"
                     /locus_tag="ACHKCL_004761"
     CDS             1628..1897
                     /gene="stxB1a"
                     /locus_tag="ACHKCL_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5765557.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2408..>2926
                     /locus_tag="ACHKCL_004762"
     CDS             2408..>2926
                     /locus_tag="ACHKCL_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5765558.1"
                     /translation="MAFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKK"
BASE COUNT          767 a          574 c          750 g          835 t
ORIGIN      
        1 ctgacacctg tataggtaaa cgccttcaca aagcggaggg gattgttgaa ggcatgctga
       61 tgatgctggg agtgaggctt gagatggatc ggtatgttga gcgtgaattg ccgggaggga
      121 gaacctctgt attttatcag cgaaaaaata gtttacgatc gtaaaaatct gcatatcatg
      181 ataagagtgg ttacattgcc acgctgctta acccgccgat gcgcgggttt ttttgtaccc
      241 agaatcctgt gagctatacg gaaagtacac agaaaggaag gtgcgaccac aattaataac
      301 aaaatcttaa aaattgcaca tggcactatt agttttctaa atattgtgta ttttttgtat
      361 tgcaggatga ccctgtaacg aagtttgcgt aacagcattt tgctctacga gtttgccagc
      421 ctcccccagt ggctggcttt tttatgtccg taacatcctg tgtatcaata aatgttgttg
      481 tctacgtacg tcaagtagtc gcatgagatc tgaccagata tgttaaggtt gcagctctct
      541 ttgaatataa ttatcatttt cattacgtta ttgttacgtt tatccggtgc gccgtaaaac
      601 gccgtccttc agggggtgga ggatgtcaag aatatagtta tcgtatggtg ctcaaggagt
      661 attgtgtaat atgaaaataa ttatttttag agtgctaact tttttctttg ttatcttttc
      721 agttaatgtg gttgcgaagg aatttacctt agacttctcg actgcaaaga cgtatgtaga
      781 ttcgctgaat gtcattcgct ctgcaatagg tactccatta cagactattt catcaggagg
      841 tacgtcttta ctgatgattg atagtggcac aggggataat ttgtttgcag ttgatgtcag
      901 agggatagat ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa
      961 tttatatgtg acaggatttg ttaacaggac aaataatgtt ttttatcgct ttgctgattt
     1021 ttcacatgtt acctttccag gtacaacagc ggttacattg tctggtgaca gtagctatac
     1081 cacgttacag cgtgttgcag ggatcagtcg tacggggatg cagataaatc gccattcgtt
     1141 gactacttct tatctggatt taatgtcgca tagtggaacc tcactgacgc agtctgtggc
     1201 aagagcgatg ttacggtttg ttactgtgac agctgaagct ttacgttttc ggcaaataca
     1261 gaggggattt cgtacaacac tggatgatct cagtgggcgt tcttatgtaa tgactgctga
     1321 agatgttgat cttacattga actggggaag gttgagtagt gtcctgcctg attatcatgg
     1381 acaagactct gttcgtgtag gaagaatttc ttttggaagc attaatgcaa ttctgggaag
     1441 cgtggcatta atactgaatt gtcatcatca tgcatcgcga gttgccagaa tggcatctga
     1501 tgagtttcct tctatgtgtc cggcagatgg aagagtccgt gggattacgc acaataaaat
     1561 attgtgggat tcatccactc tgggggcaat tctgatgcgc agaactatta gcagttgagg
     1621 gggtaaaatg aaaaaaacat tattaatagc tgcatcgctt tcattttttt cagcaagtgc
     1681 gctggcgacg cctgattgtg taactggaaa ggtggagtat acaaaatata atgatgacga
     1741 tacctttaca gttaaagtgg gtgataaaga attatttacc aacagatgga atcttcagtc
     1801 tcttcttctc agtgcgcaaa ttacggggat gactgtaacc attaaaacta atgcctgtca
     1861 taatggaggg ggattcagcg aagttatttt tcgttgactt agaatagctc agtgaaaata
     1921 gcaggcggag attcataaat gttaaataca tctcaattca gtcagttgtt gccggtctga
     1981 taatagatgt gttagaaaat ttctgcatgg tgaatccccc tgtgcggagg ggcgactggt
     2041 gaacggtatg atctctttga tgatcgtaag cgagaatacg cgggtttggt ggcaccaggc
     2101 cgaactcacc gggaggcacc cggcaccatg caatggcaca tagcgccact ctccagcccc
     2161 tctccggagg ggctttctta tggacaaaaa aagcccgcgc agggagacgc tggcggcaag
     2221 gaataaacaa caaaacgtga agtaatattt cagctggcga ataatatccg acagtaatca
     2281 ctctgcgcaa tagcgcggcc tttttcgtat tgcgggctgt tgtctctctt ctgccattgt
     2341 cctgtaactt ccggatttca gcccgctcat cattttactc acaatattat cccggccggg
     2401 aggattcatg gcatttaaac actacgatgt ggtcagggcg gcatcgccgt cagaccttgc
     2461 tgatgcactt gcgcaaaaaa ttcgtgaagg atggcaacca tacggtgggc cgttttcttc
     2521 gtatacggat gatggcgcag cacttattca ggcgattgtc gcagaaggtg atgtgagcac
     2581 acctgttgtg gtgaagctga caggtggaga aggtgcagta atcagcgcca ccagcgaccc
     2641 cgggtattac tttgttgtgg ttctggcagg gcagtcaaac ggcatgtcgt atggtgaagg
     2701 tcttccgctg ccggagacat atgaccgtcc ggacccgcgc attaagcagc tggcgcgtcg
     2761 cagtacggtg acaccgggcg gtgtcgcctg taaatataac gacatcattc cggcggacca
     2821 ttgtctgcat gatgtgcagg acatgagccg ccttaaccat ccgaaagcgg acctgtcaaa
     2881 ggggcagtac ggaaccgtgg ggcaggggct gcatatcgcc aaaaaa
//
LOCUS       DAZFKY010000314         2897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFKY010000314 DAZFKY010000000
VERSION     DAZFKY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            297..1022
                     /locus_tag="ACHKCL_004763"
     CDS             297..1022
                     /locus_tag="ACHKCL_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765559.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            980..1657
                     /gene="bglJ"
                     /locus_tag="ACHKCL_004764"
     CDS             980..1657
                     /gene="bglJ"
                     /locus_tag="ACHKCL_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5765560.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1695..2483)
                     /gene="fhuF"
                     /locus_tag="ACHKCL_004765"
     CDS             complement(1695..2483)
                     /gene="fhuF"
                     /locus_tag="ACHKCL_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5765561.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2624..2860
                     /locus_tag="ACHKCL_004766"
     CDS             2624..2860
                     /locus_tag="ACHKCL_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5765562.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          781 a          604 c          715 g          797 t
ORIGIN      
        1 ggaagagata tcatttaatt ataaattaaa tgaatgagat ttctgttttt cattaagtat
       61 ttctaacaac ttaatgtaga tattttaaat gtctccaggc tatttcacca ggttatcatt
      121 tgatgctaac cttctgacac atgcagtgga gttgttgtgc agcaggagta tgctgatatg
      181 aaagtgaatg ctaaggataa tttattcgct taatctatta atttgctgga aacatttaag
      241 gaatgaatat cctgaataaa ccatcataat ttttagacag tggatgtgga ggaaatatgt
      301 tgccaggatg ctgcaaaaat ggaattgtta tcagtaaaat acctgttatg caagcagggt
      361 taaaagaggt catgaggact cacttccctg aatatgaaat aatatccagc gcctctgcgg
      421 aggaccttac cttattacaa ttacgtcgtt ccggattagt cattgctgat ttagccggtg
      481 aaagtgaaga tccacgttct gtttgtgaac attattattc tttaatctca caatatcggg
      541 aaattcactg ggttttcatg gtgtcacgct cttggtattc ccaggctgta gaactgctca
      601 tgtgccctac ggcgacgtta ttgtctgatg ttgaacccat tgagaatctg gtcaagaccg
      661 tacgttccgg caatacgcac gcagagcgta tcagcgccat gctgacctct ccggcaatga
      721 ctgaaactca tgattttagc tatcgctccg tcattctcac tctttcagag cgcaaggtac
      781 tgcggctatt aggtaaagga tggggcatca accagatagc ttcattgctt aagaaaagta
      841 ataaaactat cagcgcccaa aaaaacagtg cgatgcgtcg actggcaatt cacagcaacg
      901 ctgaaatgta tgcatggata aatagcgcgc agggtgcaag agaacttaac ttgccttctg
      961 tttatggaga tgccgcagaa tggaacacag ccgaattaag aagagaaatg tcgcactcat
     1021 agaaaaatgc gtcatgagta gtatcggtat tgagagttta ttcagaaagt ttgcgggcaa
     1081 cccttataag ctccatacct ataccagtca ggagtcattt caggatgcca tgtcgcggat
     1141 ctcgtttgcg gcggtcattt tttctttttc tgccatgaga agtgagcgca gagagggatt
     1201 atcttgcctg actgaactgg cgattaagtt tccgcgtacc cggcgtttag ttattgcgga
     1261 tgatgatatt gaagctcggc tgattggttc attgtcgcca tcaccgctgg acggtgtatt
     1321 aagtaaagcg tcaacgctgg agatttttca tcaggaactc tttttgtcat taaatggtgt
     1381 acgtcaggcg accgaccgac tgaacaatca gtggtacatt aaccaaagcc ggacgttaag
     1441 cccgacggag agagaaatat tgcgctttat gtcgcgtggc tactcaatga cacaaattgc
     1501 cgagcagctt aaacgcaata tcaaaacgat ccgtgcacat aaatttaatg tgatgtcgaa
     1561 actgggcgtc agttctgacg cagggttgtt ggaggccgca gatattctgt tatgtatgcg
     1621 gcattgcgaa acaagtaatg tgttgcatcc ctattaatcc gcatgatgcc gggtttactt
     1681 ccccggcagt gctttcattt cagcgtacaa tcgccacatt gctgtacatc cggtaagcga
     1741 taacgctggc agcaagtgcg gcgcaccagc aggccgtcgc gtagcaccac ggtacgccag
     1801 agtggattat cttcaccggt agtaagcgtt ttctcaaaaa agagggcatg gcgcagcgat
     1861 tcaaccgtcg cctcgccgag cagttgcttc atctcagtaa gataccagtt gatcaaataa
     1921 ccggtattac tccagataag tttgccgttg atctctccgg tcgcttctag tgcctgcaca
     1981 accggaacca gcgcctggct gattaacgtt tccattcgct gctgcggcga atgtggtgtt
     2041 gcgtttttat cttcacacac atcgacccag aaacaggcga cgcgtccggt ttcgtgaaac
     2101 tcagcatgga aatgttccgg cgacacatct aatgcttttt cctgcgtcag tagcgccagc
     2161 attaatggtg gcaccatcag gccgatatac cattgtgccc atagtgagat cagcggtttg
     2221 tactcgcgga tcatcgttgg ttggttgcga tagatatgat cggaatagac cgccagtaga
     2281 gaacttagcg cattcggtga tgaccattgc gccagcgtca tggcgttaag cggagcgggt
     2341 tcatccaggc ggataaactc cagcaaatgt tcacgatgtt cggcgatcgt cgcacgtacg
     2401 gcttgtgcaa gcctagcatc ctgtggctgg agatgcgttc gccagatgat atcttcatag
     2461 agcggtgcgg aacgataggc cataatcggg atagtaatct aaatgataat gattgctaat
     2521 cctagcgata ggtttacccg atagcaaggg atttctctgg cttgcaagtg ataaaaatta
     2581 tcatatgata ttggttatca ttatcaatga aagagataaa accatgttgc aacgtacgct
     2641 gggcagtggc tggggagtgt tgctgccggg atttctgatt gcagggctga tgtatgcgga
     2701 tttatcgccg gatcagtggc ggattgtcat tctgatggga ttagtattga cgccggtgat
     2761 gctgtatcac aaacagttgc ggcattacat tttgctacca tcgtgcctgg cgcttattgc
     2821 tggcatcatg ctgatgataa tgaatttgaa tcagggatga aaaaacaggg aagaaacaag
     2881 aaaggaagta aagataa
//
LOCUS       DAZFKY010000315         2895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFKY010000315 DAZFKY010000000
VERSION     DAZFKY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1890
                     /locus_tag="ACHKCL_004767"
     CDS             <1..1890
                     /locus_tag="ACHKCL_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5765563.1"
                     /translation="LAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVP
                     GTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTYDRPDPRIKQLARRSTV
                     TPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGRPLYKDLIGRTKAALAK
                     NPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLADMVGQCAGGSADGVPWI
                     CGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPA
                     SGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILLHAGRTAELVGGEQVVM
                     PPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWGAEGGRALVVDDAGAAG
                     GKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDGTLTANQYALALYWPVS
                     SLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNTKLGSFGAFDNEWHTLG
                     FRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITSGATYPVLIESITV
                     EVNNP"
     gene            2038..2220
                     /locus_tag="ACHKCL_004768"
     CDS             2038..2220
                     /locus_tag="ACHKCL_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5765564.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2258..2527
                     /locus_tag="ACHKCL_004769"
     CDS             2258..2527
                     /locus_tag="ACHKCL_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5765565.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2603..2818
                     /locus_tag="ACHKCL_004770"
     CDS             2603..2818
                     /locus_tag="ACHKCL_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5765566.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2823..>2895
                     /locus_tag="ACHKCL_004771"
     CDS             2823..>2895
                     /locus_tag="ACHKCL_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO5765567.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVA"
BASE COUNT          730 a          671 c          866 g          628 t
ORIGIN      
        1 cttgcggaaa agctgacaca caaactgaaa gagggctggc agccgtttgg tagtcctgtg
       61 gccataaccc cttataccct gatgcaggcg attgccgcgg agggggatgt gaccacgcca
      121 gtggttgtgc ccggcacggg ggatggtggc tatccgggag tggtcaccac ggagccagat
      181 tattactacg ttattccact ggccgggcag tcgaacggca tggcttacgg tgaggggctt
      241 cctctgccgc agacatatga ccgtcctgac ccgcgtataa agcaactggc tcgtcgcagc
      301 acagtgacgc cggatggcgc tccctgtaaa tataacgaca ttattccggc agaccactgt
      361 ctgcatgatg tacaggacat gagccgtctt aaccatccga aagctgacct gtcgaaaggt
      421 cagtacggaa ccgtggggca ggggctgcat attgccaaaa agctgctgcc gtttataccg
      481 gcgaatgcgg gtattcttct ggttccgtgc tgccgtggtg gttcagcttt caccaccggg
      541 gcagatggaa catacagtga cgtgaccggt gcctcagaga gttctacccg ctggggtgtg
      601 ggcaggccgc tgtataagga tctcatcggt cgtacaaaag ccgcgctggc aaagaacccg
      661 aaaaatgtgc tgcttgccgt ggtgtggatg cagggggaat ttgactttga cggaacgcca
      721 gcaaatcaca cagcccgttt tacagaagta gtggaacaat atcgtacgga ccttgcagat
      781 atggtgggac agtgcgctgg tggttctgct gacggtgttc cctggatatg tggagacaca
      841 acttatttct ggaagcagaa gagcgaatcc acttaccaga cggtgtacgg cagttacaaa
      901 aacaaaacgg aaaagaatat tcactttgtg ccgttcatga ccgatgagaa cggagcaaat
      961 gtcccgacga acaaaccgga agaagacccg gatattccgg catcaggata ttacggtgcg
     1021 gcctcccgga cgtcggcaaa ctggacgtca gcagaccgtg cgagccattt cagctcatgg
     1081 gcacgcaggg ggattatttc tgaccgtctt gcctcagcga ttcttctcca tgcaggacgg
     1141 acggctgaac tggtgggtgg ggaacaggtt gtgatgccgc cggatgagaa gccgtcaccg
     1201 gacacaccat caacaccgtc aacggacggg aaatcagtga caacgctgct ttattaccgt
     1261 gcaacagagt caggtggttt actgaatccg cagggatggg gagctgaagg agggcgtgca
     1321 ttggtagttg atgatgcagg tgctgcagga ggtaaggcgc tgaggtggac caaacagaca
     1381 ggaagttcct cgtggtttat gcagcatgat gccggtaatg gcgcagacct gctggagaag
     1441 ggcgggctta tcagttgtcg ttttaaagtt gatggcacac tgacagctaa tcagtacgca
     1501 ctggcgctgt actggccggt ttcttcactg cctcagggcg tcacactgga aggtaatgcc
     1561 ggtcataacc tgctggcgtc gttttacgta cagagcgatg ccacagacct taatgtgatg
     1621 taccacaagg gaaatgctgg tcagaacacg aagctggggt cattcggcgc atttgataac
     1681 gaatggcata cgctgggctt ccgttttgcc ggtaacaaca gtattgaggt gacgccggtc
     1741 attgatggta aggacgggac gccgttcatg ctgtcacagt caccggtcgg cacgtttacg
     1801 gcagacaaat tgcgcgtgac cgatatcact agcggtgcga catatccggt gctgattgaa
     1861 agtataacag tggaagtgaa taacccgtaa gcaggaaaaa aaggccgccg gggcagggaa
     1921 aacaaggagc cagaaccggc ggcaaatgtc gttatatcca aagcaaaaca tgcaggacac
     1981 ttttttaacc aacaggtatt aacgatgtca acaccatatc aataaccggg agggataatg
     2041 agatttgtac agcttatttt attgtatttc tgcacggtgg tgtgcacgtt atatctggta
     2101 agtggcgggt ataaggttat ccggaactat atacgcaaaa agattgatgc cgcggcggcg
     2161 gaaaaaatca gcgccagcca gtcagccgga acaaaacccg aagagcctct catttcgtag
     2221 caactttctt aacaacacct ttcaacgaga aaatcccatg tcagaaataa aatctctggt
     2281 cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcagaagcg cactgaaaca
     2341 gcaactccgc cagaatcttg aggcgcgtct tgatgctgaa gtggatgcca ttctggatga
     2401 gctgctgggg ggaccggctg ctcctgagcc tgaagacggc gcgggtgaca gtgctgtttc
     2461 agatggcgtt gtgtctcagc ctgacggtag cagtgagcct cagcctggcg gcgaaatgat
     2521 gatgtaacca tacgcagggg ctgtcggtgt gagctgatgc ccctcctgtt gttgtgagct
     2581 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata
     2641 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
     2701 gtcccagtgg gtggcgatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
     2761 gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag
     2821 cgatgaagaa aaaatacgaa ctggttgtta aagggataaa taattacccg gataagatta
     2881 ctgttactgt ggcac
//
LOCUS       DAZFKY010000316         2855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFKY010000316 DAZFKY010000000
VERSION     DAZFKY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1437
                     /locus_tag="ACHKCL_004772"
     CDS             <1..1437
                     /locus_tag="ACHKCL_004772"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO5765568.1"
                     /translation="PIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRL
                     SGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHALKRN
                     PQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGYIERG
                     ELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRK
                     FRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGW
                     SDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVIVQTKD
                     AVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVK
                     PGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPGIIP
                     LNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            1441..2811
                     /gene="cpsG"
                     /locus_tag="ACHKCL_004773"
                     /gene_synonym="manB"
     CDS             1441..2811
                     /gene="cpsG"
                     /locus_tag="ACHKCL_004773"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5765569.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT          815 a          598 c          691 g          751 t
ORIGIN      
        1 ccaataattg ctgtagttat ggccggtggt acaggcagtc gtctttggcc actttctcgt
       61 gaactatatc caaagcagtt tttacaactc tctggtgata acaccttgtt acaaacgact
      121 ttgctacgac tttcaggcct atcatgtcaa aaaccattag tgataacaaa tgaacagcat
      181 cgctttgttg tggctgaaca gttaagggaa ataaataaat taaatggtaa tattattcta
      241 gaaccatgcg ggcgaaatac tgcaccagca atagcgatat ctgcgtttca tgcgttaaaa
      301 cgtaatcctc aggaagatcc attgcttcta gttcttgcgg cagaccacgt tatagctaaa
      361 gaaagtgttt tctgtgatgc tattaaaaat gcaactccca tcgctaatca aggtaaaatt
      421 gtaacgtttg gaattatacc agaatatgct gaaactggtt atgggtatat tgagagaggt
      481 gaactatctg taccgcttca agggcatgaa aatactggtt tttattatgt aaataagttt
      541 gtcgaaaagc ctaatcgtga aaccgcagaa ttgtatatga cttctggtaa tcactattgg
      601 aatagtggaa tattcatgtt taaggcatct gtttatcttg aggaattgag aaaatttaga
      661 cctgacattt acaatgtttg tgaacaggtt gcctcatcct catacattga tctagatttt
      721 attcgattat caaaagaaca atttcaagat tgtcctgctg aatctattga ttttgctgta
      781 atggaaaaaa cagaaaaatg tgttgtatgc cctgttgata ttggttggag tgacgttgga
      841 tcttggcaat cgttatggga cattagtcta aaatcgaaaa caggagatgt atgtaaaggt
      901 gatatattaa cctatgatac taagaataat tatatctact ctgagtcagc gttggtagcc
      961 gccattggaa ttgaagatat ggttatcgtg caaactaaag atgccgttct tgtgtctaaa
     1021 aagagtgatg tacagcatgt aaaaaaaata gtcgaaatgc ttaaattgca gcaacgtaca
     1081 gagtatatta gtcatcgtga agttttccga ccatggggaa aatttgattc gattgaccaa
     1141 ggtgagcgat acaaagtcaa gaaaattatt gtgaaacctg gtgaggggct ttctttaagg
     1201 atgcatcacc atcgttctga acattggatc gtgctttctg gtacagcaaa agtaaccctt
     1261 ggcgataaaa ctaaactagt caccgcaaat gaatcgatat acattcccct tggcgcagcg
     1321 tatagtcttg agaatccggg cataatccct cttaatctta ttgaagtcag ttcaggggat
     1381 tatttgggag aggatgatat tataagacag aaagaacgtt acaaacatga agattaacat
     1441 atgaaatctt taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg
     1501 aatgaagata ttgcctggcg cattgggcgt gcctatggcg aatttctcaa accgaaaacc
     1561 attgttttag gcggtgatgt ccgcctcacc agcgaagcgt taaaactggc gcttgcgaaa
     1621 ggtttacagg atgcgggcgt cgatgtgctg gatatcggta tgtccggcac cgaagagatc
     1681 tatttcgcca cgttccatct cggagtggat ggcggcatcg aagttaccgc cagccataac
     1741 ccgatggatt acaacggcat gaagctggtg cgcgaagggg ctcgcccgat cagcggtgat
     1801 accggactgc gcgatgtcca gcgtctggca gaagccaatg acttccctcc tgtcgatgaa
     1861 accaaacgtg gtcgctatca gcaaatcaat ctgcgtgacg cttacgttga tcacctgttc
     1921 ggttatatca acgtcaaaaa cctcacgccg ctcaagctgg tgatcaactc cgggaacggc
     1981 gcagcgggtc cggtggtgga cgccattgaa gcccgattta aagccctcgg cgcaccggtg
     2041 gaattaatca aagtacacaa cacgccggac ggcaatttcc ccaacggtat tcctaacccg
     2101 ctgctgccgg aatgccgcga cgacacccgt aatgcggtca tcaaacacgg cgcggatatg
     2161 ggcattgcct ttgatggcga ttttgaccgc tgtttcctgt ttgacgaaaa agggcagttt
     2221 atcgagggct actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc
     2281 gcgaagatca tccacgatcc acgtctctcc tggaacaccg ttgatgtggt gactgccgca
     2341 ggcggcaccc cggtaatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag
     2401 gaagacgcca tctacggtgg cgaaatgagc gctcaccatt acttccgtga tttcgcttac
     2461 tgcgacagcg gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaaggaaaa
     2521 acgctgggcg aaatggtgcg cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac
     2581 agcaaactgg cgcaacccgt tgaggcaatt aatcgcgtgg aacagcattt tagccgcgag
     2641 gcgctggcgg tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaac
     2701 ctgcgctcct ccaacaccga accggtggtg cggttgaatg tggaatcacg cggtgatgta
     2761 aagctaatgg aaaagaaaac taaagctctt cttaaattgc taagtgagtg attatttaca
     2821 ttaatcatta agcgtattta agattatatt aaagt
//
LOCUS       DAZFKY010000317         2849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFKY010000317 DAZFKY010000000
VERSION     DAZFKY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            205..1158
                     /gene="ompT"
                     /locus_tag="ACHKCL_004774"
     CDS             205..1158
                     /gene="ompT"
                     /locus_tag="ACHKCL_004774"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5765570.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1345..2829
                     /locus_tag="ACHKCL_004775"
     CDS             1345..2829
                     /locus_tag="ACHKCL_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5765571.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          942 a          603 c          563 g          741 t
ORIGIN      
        1 atatcagcaa aaagaaaatc tccagcatat taaaccacaa caataataca acacttggca
       61 agctgcccat aaagaatgaa taaaacgaaa caataattca aacttaaaca aaccataaac
      121 agttaaacaa tatacaattg actcattaag ttagatataa aaaatacata ttcaatcatt
      181 aaaacgattg aatggagacc ttttatgcgg gcgaaacttc tgggaatagt cctgacaacc
      241 cctattgcga tcagctcttt tgcttctacc gagactttat cgtttactcc tgacaacata
      301 aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa aagagcgtgt ttatctagcc
      361 gaagaaggag gccgaaaagt cagtcaactc gactggaaat tcaataacgc tgcaattatt
      421 aaaggtgcaa ttaattggga tttgatgccc cagatatcta tcggggctgc tggctggaca
      481 actctcggca gccgaggtgg caatatggtc gatcaggact ggatggattc cagtaacccc
      541 ggaacctgga cggatgaaag tagacaccct gatacacaac tcaattatgc caacgaattt
      601 gatctgaata tcaaaggctg gctcctcaac gaacccaatt accgcctggg actcatggcc
      661 ggatatcagg aaagccgtta tagctttaca gccagaggtg gttcctatat ctacagttct
      721 gaggagggat tcagagatga tatcggctcc ttcccgaatg gagaaagagc aatcggctac
      781 aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa gttatcgtta tgaagatttt
      841 gagctaggtg gcacatttaa atacagcggc tgggtggaag catctgataa cgatgagcac
      901 tatgacccag gaaaaagaat cacttatcgc agtaaagtca aagaccaaaa ttactattct
      961 gtttcagtca atgcaggtta ttacgtaaca cctaacgcaa aagtttatgt tgaaggcaca
     1021 tggaatcggg ttacgaataa aaaaggtaat acttcacttt atgatcacaa tgataacact
     1081 tcagactaca gcaaaaatgg tgcaggcata gaaaactata acttcatcac tactgctggt
     1141 cttaagtaca ccttttaacg aagttaacca gattttctcc ccggtggtat tgtataaccc
     1201 cggggaattt gtttacagtt acaagtagac aatatccact aattttgtaa gcatttaacc
     1261 tatgtcataa cgtgtaatct cttaccgcaa cacaagacct caatatattc aaattttatc
     1321 tttattccga tattcagaga cacaatgcga aaaataatta ctcatttcaa agttgtttta
     1381 acgttacttc taccagtaac cgtatctgcc cagcagatac agtggcaatc ctgtatggcc
     1441 agtcaattca accactggtt tggtgaggaa aaaccgtctc ctgacttact atgtggttat
     1501 ttgtctgttc cattaaaata tacagacaca ggcggagatg cttcttatga aaaaaaatca
     1561 caagtcaaac tagcgttgac aaaattgccg gcaaaaagca agcataaagg aagtatcctg
     1621 ataataagtg gtggtcccgg gttaccaggc ataaatcctt atattaactt tgactggcca
     1681 gtcacaaatc ttcgtgagtc atgggatatt attggatttg atcctcgagg cgtcgggcag
     1741 tccactccga caataaactg ccggcaatca gatacagaga ctcaggaaaa cataaccgaa
     1801 aagcaacaag tattaaataa aattaatgcc tgtatccata ataccggagc cgaagtcatt
     1861 cgccatatcg gctctaacga ggctgtatac gatattgatc gtattaggca agccttgggg
     1921 gataaacaac tgacagccgt ggcgtattcg tatggaactc aaattgcagc cttatatgca
     1981 gaacgttttc cctacaacgt aagatctatc gttcttgatg gagtcgtcga tatcgatgac
     2041 ctggaggaca acttcacatg gcaactcaaa caggcacaga gttatcagga aacgtttgat
     2101 cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc tttcttcaga cagagataag
     2161 gcaataactc agttccatga gctattatca aaattacatc acaaaccttt attagacagt
     2221 aagggagaaa atatatcttc agatgaactc atatcattaa caacagacct tctgctatgg
     2281 cgttcatcat ggccaaccct tgcaactgcc atacgccagt tctctcaggg gattgtcagt
     2341 aatgaaattg aaactgcgct cagtgctccg atagcctcag aagagtcaag tgatgcttcg
     2401 ggggtaatcc tttgtgtaga tcagggggat gagcaattaa caccagaaga gcgaaaattc
     2461 cgaaaagagg ctcttgcgaa tgccttcccg gctattaact ttgacaatgg acgttccgat
     2521 tcacctgatt tttgtgaatt atggccaata catagcgacc tgaacaaaac tcgcctgaaa
     2581 aatactgttc tgccctctgg tttactgttt gtagcacaca aatacgaccc aacaacgccc
     2641 tggattaatg cccgtaagat ggcagagaaa ttttccagcc cgttactaac aataaatggt
     2701 gatgggcata cattagctct caccggagtt aatttatgtg tagataaagc agttgtacat
     2761 cacctgatca ctccacaaaa aatagaaaat atatactgcc caggaaattc tgaagcagaa
     2821 atacaataat ttcaaaatat tccgctatt
//
LOCUS       DAZFKY010000318         2835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFKY010000318 DAZFKY010000000
VERSION     DAZFKY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..2374
                     /gene="ydeP"
                     /locus_tag="ACHKCL_004776"
     CDS             95..2374
                     /gene="ydeP"
                     /locus_tag="ACHKCL_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5765572.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2622..2819
                     /gene="safA"
                     /locus_tag="ACHKCL_004777"
     CDS             2622..2819
                     /gene="safA"
                     /locus_tag="ACHKCL_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5765573.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSLQD"
BASE COUNT          763 a          654 c          686 g          732 t
ORIGIN      
        1 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
       61 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      121 gggtgctgcc ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      181 ggatatacgc caggatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      241 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      301 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctca
      361 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cgggacgtct
      421 cactcagcct ttgaaatatg atgctgtcag cgactgttac aagccattaa gctggcaaca
      481 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      541 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      601 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
      661 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
      721 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
      781 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
      841 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
      901 tcagttggcc agtgcctact ataacgtgcg cattggtggt gatatggcgt tgctcaaggg
      961 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagc gccgcaggtc ggccctcatt
     1021 gctggatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1081 gctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1141 cgaactggct gatgcatatg ccgctgccga acgaaccatt atctgttacg gaatggggat
     1201 cactcagcac gaacatggta cccagaatgt acagcaactg gtcaatctgc tgttgatgaa
     1261 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggccact ctaatgtaca
     1321 gggcgaccga accgtcggta tcaccgagaa accgtctgta gagtttctgg ctcgcctggg
     1381 tgagcgctat ggcttcaccc cacctcatgt acctggacat gctgcaattg ccagcatgca
     1441 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1501 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1561 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1621 tctgggacgt agcgagattg acatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     1681 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     1741 atcagagtgt gctgtggtcg cgggaatcgc gcaggcaaca ctaccccaga gcgtggtagc
     1801 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     1861 agagttcgcc gactataacc aacgcatccg tcatcccggt ggttttcacc tgataaatgc
     1921 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     1981 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2041 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2101 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2161 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2221 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2281 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2341 aagtattccg gttgaattag aaccatcaaa ttaatgcctc ttctcatttc ttctgctgtc
     2401 atccgcacag cagaagaatt cctcattgat tattatcgcg caatttgctc acatggatta
     2461 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2521 gtactgaagc accagtccta tttcctcttt tctcaagcct gttatattaa gcatactgat
     2581 taacgatttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     2641 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt gtggttatct
     2701 tattactgac ccttacgttg atagccattt tttcggcaat tgatcagctg ggtatttcag
     2761 aaatgggtcg cattgcaaga gatcttacac atttcattgt caatagtttg caagactgaa
     2821 aataaacagc aaatt
//
LOCUS       DAZFKY010000319         2826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFKY010000319 DAZFKY010000000
VERSION     DAZFKY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..326
                     /locus_tag="ACHKCL_004778"
     CDS             <1..326
                     /locus_tag="ACHKCL_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HFO5765574.1"
                     /translation="DEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNNV
                     VIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGNY
                     ISDKI"
     gene            342..2822
                     /locus_tag="ACHKCL_004779"
     CDS             342..2822
                     /locus_tag="ACHKCL_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5765575.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
BASE COUNT          821 a          530 c          708 g          767 t
ORIGIN      
        1 aagatgaagg aaagaatgcg ttaaaatttg ccatgcaaaa cagaatcaag ttgttttacc
       61 gacctgctgg tattgctccg gtaaataaaa cgacttttaa aaaattacga gtgagtcgta
      121 gcggtaataa cgtagtcata aaaaatgact cgccaaattg ggtaacgatt tctgatgtta
      181 aggctaataa cgtcaaagtg aattatgaaa caatcatgat tgcaccacaa gaaagtcaga
      241 atgttagtga aaaaaataat aatgcgaatg gctggcaact gaccattatc gatgatcacg
      301 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccgc
      361 ttgcatcagc aatagtcgcg ttattgctcg gcattgaagc acatgcagca gaagaaacct
      421 ttgacaccca ttttatgatg ggaggaatga aaggtgagca agtaacaaat ttgcgtcttg
      481 atgataatca gcctttacct gggcagtacg acattgatat ttatgtcaat aaacaatggc
      541 gtggaaaata tgagattatc gtcaaagata acccacatga aacttgcctg acgcgtgaaa
      601 ttgtaaagcg gttaggtata aatagcgata attttgcacg cgaaaatcag tgtttaacat
      661 ttgagcaact tgttcagggc ggaagctact cctgggatat tgggattttt cgtttggatc
      721 tcgctgttcc gcaagcctgg gttgaagagc tggaaaatgg ttacgttccg ccagaaaact
      781 gggagcgggg aatcaatgcg ttttatacgt cttattacgt aagtcagtat tacagtgact
      841 ataaagcctc gggcaatagt aagagcactt atgtgcgttt taacagtggg ctaaatttgc
      901 tgggatggca gctgcattca gatgccagtt ttagcaaaac ggataataat cctggtgagt
      961 ggaaaagcaa cacgctgtat ctggagcatg gattttctca aattctcggt acgctgcgta
     1021 ttggtgatat gtacacctca gccgatatat ttgattccgt tcgcttcacc ggggttcggt
     1081 tatttcgtga tatgcagatg ttgccaaact cgaagcaaaa ttttacccca cgggtacagg
     1141 ggattgccca gagtaacgcg ttggtaacta ttgaacagaa cggttttgtc gtttatcaga
     1201 aagaggttcc accaggtccg ttttcgatta gtgatttgca gttagcgggc ggaggggcgg
     1261 atcttgatgt tagtgttaag gaagccgatg gttctgtgac tacgtatctg gtgccttatg
     1321 cggcagtacc taatatgctg caacctggtg tatcaaaata tgattttgcg gcaggccgta
     1381 gtcatattga aggtgcgagc aagcaaagtg attttgtcca ggcgggttat cagtatggtt
     1441 ttaataattt attgacgctg tatggcggca cgatggttgc taataattac tatgccttta
     1501 ccctcggaac aggttggaac acgcgtattg gcgcaatttc agtcgatgca actaaatcgc
     1561 acagtaagca agacaatggt gatgtgtttg acggacaaag ttatcaaatt gcctataaca
     1621 aatttttgag ccaaacatca acacgttttg gtctggcggc ctggcgttat tcgtcgcgtg
     1681 attaccgcac atttaacgat tatgtgtggg caaataataa agataattat cgtcgtgata
     1741 aaaacgatgt ctatgatatt gccgattatt accagaatga ttttggtcgc aaaaatagct
     1801 tttccgccaa tatgagtcag tcattgccag aaggctgggg atctgtgtca ttaagtactt
     1861 tatggcgtga ttactggggg cgaagcggca gtagtaaaga ttatcaactg agttattcca
     1921 ataactggcg gcggataagc tataccctcg cggcaagcca ggcgtatgac gagaaccacg
     1981 ccgaagagaa acgctttaat atttttatat cgattccctt tgactggggc gatgacgtta
     2041 cgacgccgcg acggcaaata tatatgtcta actcaacgac ctttgatgat cagggttttg
     2101 cttcaaacaa taccggatta tcgggaaccg ttgggaaccg cgatcagttt aactatggta
     2161 tcaacttgag ccatcaacat cagggaaatg aaacgacagc tggggccaat ttgacctgga
     2221 ccgcaccggc cgcaacagtg aatggcagct atagtcagtc gagtacttat cgacaggtcg
     2281 gagccagtgt ttcaggggga ctggttgcct ggtctggtgg cgttaatctg gcgaaccgtc
     2341 tttccgaaac gtttgctgtg atgcatgcgc cgggaatcaa agatgcttat gtcaatgggc
     2401 aaaaatatcg tacaacaaac tgtaatggtg tggtggtgta cgacggactg acaccttatc
     2461 gggaaaatca cctgatgatg gatgtgtcgc aaagcgatag cgaaacagaa ttacgcggga
     2521 accgtaaaat gaccgcccct tatcgcggcg cggtcgttct ggttgatttt gataccgatc
     2581 agcgtaagcc ctggtttata aaagcattaa gatccgatgg acagccatta acgtttggtt
     2641 atgaagtcaa tgatatgcat ggtcataaca ttggtgttgt cggccagggc agtcagatat
     2701 ttattcgcac caatgaaata ccgccagcgg ttaatgtggc aattgataag caacaagggc
     2761 tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag taaaaattat atctgtcggt
     2821 gagagg
//
LOCUS       DAZFKY010000320         2819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFKY010000320 DAZFKY010000000
VERSION     DAZFKY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKCL_004780"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKCL_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO5765576.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHKCL_004781"
     CDS             complement(399..1022)
                     /locus_tag="ACHKCL_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765577.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHKCL_004782"
     CDS             complement(1025..1306)
                     /locus_tag="ACHKCL_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5765578.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHKCL_004783"
     CDS             complement(1299..1625)
                     /locus_tag="ACHKCL_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO5765579.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..>2819)
                     /locus_tag="ACHKCL_004784"
     CDS             complement(1713..>2819)
                     /locus_tag="ACHKCL_004784"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5765580.1"
                     /translation="GKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKDN
                     AYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQGW
                     EESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALAT
                     YGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFHA
                     DHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSASV
                     KGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYID
                     QQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
BASE COUNT          593 a          896 c          684 g          646 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgcc
//
LOCUS       DAZFKY010000321         2816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFKY010000321 DAZFKY010000000
VERSION     DAZFKY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..447
                     /gene="wcaM"
                     /locus_tag="ACHKCL_004785"
     CDS             <1..447
                     /gene="wcaM"
                     /locus_tag="ACHKCL_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="WcaM from Salmonella enterica is essential for
                     biofilm formation on HEp-2 cells and chicken intestinal
                     tissue but not on a plastic surface; may be involved in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5765581.1"
                     /translation="LIGYGVIKGNYLSIPQNFRVNNIQLDNTHLAYKLRGLQISAGNA
                     VSFVALTNIEMKRASLELHNKPQHLFLRNIKVMQESSVGPALIMNFDMRKDVRGVFMA
                     KEETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE"
     gene            605..1600
                     /locus_tag="ACHKCL_004786"
     CDS             605..1600
                     /locus_tag="ACHKCL_004786"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO5765582.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            1843..2736
                     /gene="galF"
                     /locus_tag="ACHKCL_004787"
     CDS             1843..2736
                     /gene="galF"
                     /locus_tag="ACHKCL_004787"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5765583.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT          823 a          613 c          668 g          712 t
ORIGIN      
        1 ttaatcggct atggggtaat taaaggcaat tatctctcga ttccgcaaaa tttccgagtg
       61 aataatattc aactggataa cacccatctt gcttataaat tgcgcggcct ccaaatctct
      121 gccgggaatg ccgtctcctt tgtggcacta actaacattg agatgaagcg tgcgtcgctg
      181 gagttacaca acaaaccgca acatcttttt ctgcgtaata tcaaagtgat gcaggaatcc
      241 tctgttggac ccgcattgat tatgaacttc gacatgcgca aagacgttcg aggcgtcttt
      301 atggcgaaag aagaaacact gctgtctctt gcaaatgttc atgcggtgaa tgagaaagga
      361 caaagctccg tcgatatcga caggattaat caccatattg ttaatgtgga aaagattaac
      421 tttagattgc cggaacggag agagtagatt tgcgaccatt cataaactta ctggatataa
      481 tgttctaaaa gattataatc ctcacactaa ctgttaaagc tggggctggg cagcattgac
      541 tactcaggga aggtactcat ctttatcttg tgtttcttac gtaacaacca gactaaagtg
      601 atttatgaac gataacgttt tgctcatagg agcttccgga ttcgtaggaa cccgactact
      661 tgaaacggca attgctgact ttaatatcaa gaacctggac aaacagcaga gccactttta
      721 tccagaaatc acacagattg gtgatgttcg tgatcaacag gcactcgacc aggcgttagc
      781 cggttttgac actgttgtac tactggcagc ggaacaccgc gatgacgtca gccctacttc
      841 tctctattat gatgtcaacg ttcagggtac ccgcaatgtg ctggcggcca tggaaaaaaa
      901 tggcgttaaa aatatcatct ttaccagttc cgttgctgtt tatggtttga acaaacacaa
      961 ccctgacgaa aaccatccac acgacccttt caaccactac ggcaaaagca agtggcaggc
     1021 ggaggaagtg ctgcgtgaat ggtataacaa agcaccaaca gaacgttcat taactatcat
     1081 ccgtcctacc gttatcttcg gtgaacgcaa ccgcggtaac gtctataact tgctgaaaca
     1141 gatcgctggc ggcaagttta tgatggtggg cgcagggact aactataagt ccatggctta
     1201 tgttggaaac attgttgagt ttatcaagta caaactgaag aatgttgccg caggttacga
     1261 ggtttataac tacgttgata agccagacct gaacatgaac cagttggttg ctgaagttga
     1321 acaaagcctg aacaaaaaga tcccttctat gcacttgcct tacccactag gaatgctggg
     1381 tggatattgc tttgatatcc tgagcaaaat tacgggcaaa aaatacgctg tcagctctgt
     1441 gcgcgtgaaa aaattctgcg caacaacaca gtttgacgca acgaaagtgc attcttcagg
     1501 ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga actctgcagt atgaattcgt
     1561 ccatgccaaa aaagacgaca taacgtttgt ttctgagtaa tgcagacgag tcagagaaaa
     1621 aaggaccagg ttggactacg attattcgga aagaggtact gattaaatta tcagtatgtg
     1681 gatttccgag taatccgagt aacccagatg ctgatggtgt gtagtgttat cgtaaggttt
     1741 aatcaaagtt atgcggaact aagctttggt atatagccat aacgtttggc taacctatgg
     1801 caataattta cgcactatca gccacttaga ggtaggaaaa gaatgacgaa tttaaaagca
     1861 gttataccgg tagcaggtct tgggatgcat atgttgcctg ccactaaggc gattcccaaa
     1921 gagatgctac cgatcgtcga caagccaatg attcagtaca ttgttgacga gattgtggct
     1981 gcagggatca aagaaatcct cctggtaact cacgcgtcca agaacgcagt cgaaaaccac
     2041 ttcgacacct cttatgaatt agaatctctc cttgaacagc gcgtgaagcg tcaactactg
     2101 gcggaagtac aatctatctg tccgccgggc gtgaccatta tgaacgtgcg tcagggtgaa
     2161 cctttaggtt taggccactc cattttatgt gcgcgacctg ccattggtga caatccattt
     2221 gtcgtggtgc tgccagacgt tgtgatcgac gacgccagcg ccgacccgct acgctacaac
     2281 cttgctgcca tgattgcgcg cttcaatgaa acgggacgca gccaggtgct ggcaaaacgt
     2341 atgccgggcg acctctctga atactccgtc atccagacca aagaaccgct ggatcgtgaa
     2401 ggcaaagtca gccgcattgt tgaatttatc gaaaagccgg atcagccgca gacgctggac
     2461 tcagacatca tggccgttgg tcgatatgtg ctttctgccg atatttggcc ggaactggaa
     2521 cgtactcagc ctggtgcatg gggacgtatt cagctgactg atgccattgc tgagctggca
     2581 aaaaaacaat ccgttgatgc aatgctgatg actggcgaca gttacgactg cggtaaaaaa
     2641 atgggctata tgcaggcgtt tgtgaagtat ggactacgca acttgaaaga aggggcgaag
     2701 ttccgcaaag gtatcgagaa gctgttaagc gaataatgaa aatctgaccg aatgtaacgg
     2761 ttgataagga aataatatcg gcagtgaaaa actatagatc aataaatatc actgct
//
LOCUS       DAZFKY010000322         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFKY010000322 DAZFKY010000000
VERSION     DAZFKY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..570
                     /locus_tag="ACHKCL_004788"
     CDS             <1..570
                     /locus_tag="ACHKCL_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765584.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHKCL_004789"
     CDS             631..>2801
                     /locus_tag="ACHKCL_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5765585.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          739 c          921 g          537 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaatcatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZFKY010000323         2795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFKY010000323 DAZFKY010000000
VERSION     DAZFKY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(35..111)
                     /locus_tag="ACHKCL_004790"
     tRNA            complement(35..111)
                     /locus_tag="ACHKCL_004790"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Asp,seq:gtc)
     gene            complement(244..975)
                     /gene="dnaQ"
                     /locus_tag="ACHKCL_004791"
     CDS             complement(244..975)
                     /gene="dnaQ"
                     /locus_tag="ACHKCL_004791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5765586.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1040..1507
                     /gene="rnhA"
                     /locus_tag="ACHKCL_004792"
     CDS             1040..1507
                     /gene="rnhA"
                     /locus_tag="ACHKCL_004792"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5765587.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1504..2226)
                     /locus_tag="ACHKCL_004793"
     CDS             complement(1504..2226)
                     /locus_tag="ACHKCL_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5765588.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2260..>2795
                     /gene="gloB"
                     /locus_tag="ACHKCL_004794"
     CDS             2260..>2795
                     /gene="gloB"
                     /locus_tag="ACHKCL_004794"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5765589.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIL"
BASE COUNT          724 a          687 c          677 g          707 t
ORIGIN      
        1 gcggttctct gaactcattt tcatgaggtg aatttggcgg aacggacggg actcgaaccc
       61 gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc cgcgttgtgt
      121 tccgttggga acgaggcgaa tattacggat tgcctcaccc ttcgtcaacg gtttttctca
      181 tcttttgaat catttgctgc aaaaatcgcc caagtcgcta tttttagcgc cttttacatg
      241 tatttatgcc cgccagaggc agcttccgcc ctttttctgc accagatcga gacgggcttc
      301 atgtgctgca agctcttcat ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac
      361 aaggcgctgg attgttgctt caccttgttg ctgttgtgtc tctccttcca tcgcaaaagc
      421 catcgacgtt tgaccaccgg tcatcgccag ataaacttcc gcaaggatct gggcatcgag
      481 taatgccccg tgcagcgttc gtttactgtt atctatttcg tagcgggcac ataacgcatc
      541 gaggctgttg cgtttaccgg gaaacatttt cctcgccacc gcaaggctat cggtgacctt
      601 acagaaggta ttagtcttcg gaatatcgcg cttaagcaac gagaactcgt agtccataaa
      661 gccgatatcg aacgctgcgt tatggatcac caactccgcg ccgcggatat agtccatgaa
      721 ctcatcggct acttcggcaa acgtgggctt atcgagcaaa aattcatcgg caataccatg
      781 tacgccaaag gcttccggat ccaccagccg atcgggtttg agataaacat ggaagttatt
      841 gcccgtcagg cgacggttca ccacttcaac ggcaccaatc tcaatgatct tgtggccttc
      901 atagtgcgca ccaatctggt tcataccggt ggtttcggta tcgagaacga tctggcgtgt
      961 aattgcagtg ctcatagcgg tcatttatgt cagacttgtc attttacagt tcgattcaat
     1021 tacaggaagt ctaccagaga tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct
     1081 gggtaatcct ggacctgggg gttacggcgc tattttacgc tatcgcggac gcgagaaaac
     1141 ctttagcgca ggctacaccc gcaccaccaa caaccgtatg gagttgatgg ccgctattgt
     1201 cgcgctggag gcgttaaaag aacattgcga agtcattttg agtaccgaca gccagtatgt
     1261 ccgccagggt atcacccagt ggatccataa ctggaaaaaa cgtggctgga aaaccgcaga
     1321 caaaaaacca gtaaaaaatg tcgatctctg gcaacgtctt gatgctgcat tggggcagca
     1381 tcaaatcaaa tgggaatggg ttaaaggcca tgccggacac ccggaaaacg aacgctgtga
     1441 tgaactggct cgtgccgcgg cgatgaatcc cacactggaa gatacgggct accaagtcga
     1501 agtttaagcc tgtggtttac gacattgccg ggtggctcca accgcctggc gaattcgtgg
     1561 cttgttttta ctctgtttca tcggatttag cgttaaagga atagtccgtt tccgggcaac
     1621 aataagttgt aagcaaccaa gcgcaggaat atgcgcattc aaaagttttc ctccgtgttt
     1681 gttccacggg agaacgtgga aacggctggc gtgtagcact tcaaaattca acaaagagag
     1741 ccagtccagt tgccgcatca gagtaaacat ccggctgtta tagggcgagg ttttgcgcaa
     1801 taccggcaca agtttgcgta atcccattaa actaatggga ttgaagccac taatgaccag
     1861 ccagccatca tcaatcaata cccgatcggc ttcacgcaat aaacgatgcg gatcggtgca
     1921 ccacggcaat gtatgtgcca gtagacaaac atcaacggat ttatcggcaa aaggaagatg
     1981 aagtgggtcc gcctgcacct ggacaggcat tccttgcgca gaaacattca cttgatgaga
     2041 aaccgcgcac gcttcgcaat tgatttctgc gcttaaattg ccaatcttaa gcagataaaa
     2101 accatacatt ttagtgaacc acgggttgag ctggcgctcc agcgccttgc gataaagctc
     2161 tccccagggc aaatcgcccc agcgatcagg agccacgaca gtttgaggga ctcttgccgg
     2221 tttcatcaca accttccgtt tcacactgag aggtaatcta tgaatcttaa cagtattccc
     2281 gcctttgatg acaattacat ctgggttttg aatgatgaag caggtcgctg cctgattgtc
     2341 gatcccggag acgcagagcc agtattaaac gccattgccg ccaataactg gcaaccggag
     2401 gccatatttc tcacccacca tcatcacgat cacgttggcg gcgtaaaaga actggtggaa
     2461 aagtttccgc aaattgtggt gtatggtcca caagagacac aagataaggg aacaacacag
     2521 gtagtcaaag atggcgaaac tgccttcgtt ttggggcatg aatttagtgt aattgccacg
     2581 ccgggtcaca ctttaggaca tatctgttac ttcagtaaac cttatctatt ttgcggcgac
     2641 acgctgtttt ctggtgggtg tggtcggttg tttgaaggga cagcatcaca aatgtatcaa
     2701 tcacttaaaa agttaagtgc gttacctgac gatacattgg tatgttgtgc tcatgaatat
     2761 acgttatcaa atatgaagtt tgctttgagt attct
//
LOCUS       DAZFKY010000324         2779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFKY010000324 DAZFKY010000000
VERSION     DAZFKY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(444..617)
                     /locus_tag="ACHKCL_004795"
     CDS             complement(444..617)
                     /locus_tag="ACHKCL_004795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765590.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            795..998
                     /locus_tag="ACHKCL_004796"
     CDS             795..998
                     /locus_tag="ACHKCL_004796"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO5765591.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(930..1403)
                     /locus_tag="ACHKCL_004797"
     CDS             complement(930..1403)
                     /locus_tag="ACHKCL_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765592.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1323..2063
                     /locus_tag="ACHKCL_004798"
     CDS             1323..2063
                     /locus_tag="ACHKCL_004798"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO5765593.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2297..2779
                     /locus_tag="ACHKCL_004799"
                     /pseudo
     CDS             2297..2779
                     /locus_tag="ACHKCL_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          736 a          690 c          653 g          700 t
ORIGIN      
        1 cctgacgatt tgaacacact gacaaaaaac caagcatggt aataaaccga actccccaca
       61 aacttctgtt gcctcagata ctcccccccc tttcctcagg cacaaatgtt gttccataac
      121 gaaccagccc tgttccttta aactctgcat caatggcata atgtatactc accagactga
      181 gtttgctcct gcatcattat ttcgatacgt taatgcattg ttcattgcat ccggcattcc
      241 ccatcgaata tttactttgt tatatcagtc agcccataac caggtaaatt tttaaggctt
      301 actgactgaa tattcaaaat gtgttacatg ttatcaaacc tttaaagaat aacctgctct
      361 tcttcattgg ccggatttag ctcaatcttg tcagcaaaac taatataaag ccgttcataa
      421 agagaaggat gtagcttcgt tatttatctt ttacatgtaa cagtgccagg ggatattccg
      481 cacatctctg tggctatcat ccccaccacg tctccagtgg cagcacctgc ggcactggca
      541 cgggcgttat ttccctgtac caccgccagt gcagcattca ccacggcatg agctgccaca
      601 tttgcagtct tattcaccac acctgtaacc tgtctcttat acacaaatcc ccacccgggg
      661 atttgtgatg tctgtcagtc tgctttaggg ggactctttc catccggtag tttccttcct
      721 ccccggcagc gattttttga actttttctt cacccgttga tcttctctta tatttcattt
      781 tcttcgggtc tgttatggta ctttctgatt gttattcact ggctaacgag cagtctggac
      841 atgcccgtct cggtgatccg cgtcgtacac gacgccttgt gtcactgacc tcttctctcg
      901 ctcagcatgc aggtctttct attgtgaaat catcccactt cactgctcag gttgaaggtg
      961 cgtaccgcct gatacgtaac ccgtcggtgt ccccgtaagc cattgccgaa gccggattta
     1021 ctgccactgc aagggcatgt gaggcacatc cgctgctcct cgcgctggaa gataccacca
     1081 ccattaactt cggtcacagt acggcatttg atgacctggg gaataccacc actaacccga
     1141 agacgcacgg gctgctggct cactctgtcc tgatgtacgc ccccgacagc gccctgccag
     1201 tcgatcttat cgaacagcag tgctggagtc gtgccacgga cacctacggt gttaagcacc
     1261 agcgaaaaga acgtccctgt gaagaaaaag aaagttaccg ctggcagcag gcttcggagc
     1321 gcatggccga acggctgagg gagatacaga aacaggtaat cactgtctgt gaccgggaag
     1381 cggatatctg gcattacctg cattataaag tcagccgcgg gaagccacac gctgaatgtg
     1441 atgcaaaaag gcgggcgggc agcccgccag gcccggatgc tcatcagtta cagcgaagtc
     1501 agcataaaaa atcccgacaa cagcggccag gcgctctcgc tcacgtatgt ctgttgcggg
     1561 gagcaggaga ggacggagcc tgctggtatc cgctgacatc agaaaaagtg gcgagtgccg
     1621 ccgatgcgcg acgtatcgtc agccattacg agcaacgctg gctgatagag gaataccaca
     1681 aagcgtggaa aagtggtggt acatgtgtgg aatcgctgcg aatgcagacc cgggataacc
     1741 ttgagcgcat ggtggttatc gcggtgcggg tgctgggcct gcggcaggga ggtatcagtg
     1801 aagaaacaca aaatgacagc tgtgagaaaa tactgacgcc gacagagtgg aaactactgt
     1861 gggttaagct ggaaggaaag cagcttccgg cgcaaacacc gaccctgaaa tgggcctgtt
     1921 tgaaactggc taaactgggg cgatggcatg acagcaaacg cacaagcagc cccggttggg
     1981 tagttatgtg ggatggctgg ttcagacatc aggatatggc tgagggttac ctggtgatga
     2041 agtctcttga tcaggagatc tgatcaagag acaggtcaaa gatgggggac aacgtctggt
     2101 gctgtataac agcacaggcc tcacactctt gacgcccgtg gaatatatta gagaacaaaa
     2161 atttcaatat gtggtggacg catacagagg attgacagca ttcccatact aattgttaag
     2221 attcaaattg aagtatatct atctgtcatt ttgcaaatga tatgcggtag ctttacctaa
     2281 ctatcatgaa catttcgaaa aatcatccac aaagccccga agaataaagc actatcgccc
     2341 gataaagacc tgtaagctca gtggcatgga tactattgag ctcagaatgg cgagctgtac
     2401 cgttatttca ttaccatgct cgacgaacac agcaactact ctctggccgt actatcgctt
     2461 aaaagtgata tcatcaggca tttcttctgc tgtgtagccc agttgttcct tgttggtatc
     2521 aaccagatca tcactgatga tgggcaaaga gttctcggga aaattcgaca aaacgctaca
     2581 ggaagccacc gtcgggttcg tatggactta tctctatacg ccgaaaataa atgccataag
     2641 tgagcatttt aaccgaacac tgagggaaca atttattgag tttaatgaag aattgctctt
     2701 tgaatagctg aatatgttca atctgaaact ggctgagtat ctggtactgt acaacagtaa
     2761 aattcttcat aaatcgttt
//
LOCUS       DAZFKY010000325         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFKY010000325 DAZFKY010000000
VERSION     DAZFKY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..546
                     /locus_tag="ACHKCL_004800"
     CDS             <1..546
                     /locus_tag="ACHKCL_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765594.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHKCL_004801"
     CDS             607..>2777
                     /locus_tag="ACHKCL_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5765595.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZFKY010000326         2752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFKY010000326 DAZFKY010000000
VERSION     DAZFKY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..133
                     /locus_tag="ACHKCL_004802"
     CDS             <1..133
                     /locus_tag="ACHKCL_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5765596.1"
                     /translation="WLFYVRSVKAAMALGRRAIGVELESGRFEQTVREVQNVVSQNG"
     gene            174..249
                     /locus_tag="ACHKCL_004803"
     tRNA            174..249
                     /locus_tag="ACHKCL_004803"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Ile,seq:cat)
     gene            259..335
                     /locus_tag="ACHKCL_004804"
     tRNA            259..335
                     /locus_tag="ACHKCL_004804"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Arg,seq:tcg)
     gene            349..425
                     /locus_tag="ACHKCL_004805"
     tRNA            349..425
                     /locus_tag="ACHKCL_004805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Arg,seq:tct)
     gene            516..1475
                     /locus_tag="ACHKCL_004806"
     CDS             516..1475
                     /locus_tag="ACHKCL_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO5765597.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1487..1756
                     /locus_tag="ACHKCL_004807"
     CDS             1487..1756
                     /locus_tag="ACHKCL_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO5765598.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            2243..>2752
                     /locus_tag="ACHKCL_004808"
     CDS             2243..>2752
                     /locus_tag="ACHKCL_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5765599.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGGALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT          697 a          609 c          717 g          729 t
ORIGIN      
        1 ctggcttttt tatgtccgta gcgtcaaagc agcaatggcg ctagggcgtc gtgcaattgg
       61 cgttgagctg gagagcgggc gttttgagca gacggtcagg gaagttcaga atgtagtcag
      121 tcagaacgga tgatattgca ggattagtta cgtaccgtta ttatcctgcg cccggccctt
      181 tagctcagtg gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa
      241 gggccaccat atcacatacc gccattagct catcgggaca gagcgccagc cttcgaagct
      301 ggctgcgcgg ggttcgagtc ctcgatggcg gtccattatc tgcattatgc gttgttagct
      361 cagccggaca gagcaattgc cttctgagca atcggtcact ggttcgaatc cagtacaacg
      421 cgccatattt atttaccagg ctcgcttttg cgggcctttt ttatatctgc gccgggtctg
      481 gtgctgatta cttcagccaa aaggaacacc tgtatatgaa gtgtatatta tttaaatggg
      541 tactgtgcct gttactgggt ttttcttcgg tatcctattc ccgggagttt acgatagact
      601 tttcgaccca acaaagttat gtctcttcgt taaatagtat acggacagag atatcgaccc
      661 ctcttgaaca tatatctcag gggaccacat cggtgtctgt tattaaccac accccaccgg
      721 gcagttattt tgctgtggat atacgagggc ttgatgtcta tcaggcgcgt tttgaccatc
      781 ttcgtctgat tattgagcaa aataatttat atgtggccgg gttcgttaat acggcaacaa
      841 atactttcta ccgtttttca gattttacac atatatcagt gcccggtgtg acaacggttt
      901 ccatgacaac ggacagcagt tataccactc tgcaacgtgt cgcagcgctg gaacgttccg
      961 gaatgcaaat cagtcgtcac tcactggttt catcatatct ggcgttaatg gagttcagtg
     1021 gtaatacaat gaccagagat gcatccagag cagttctgcg ttttgtcact gtcacagcag
     1081 aagccttacg cttcaggcag atacagagag aatttcgtca ggcactgtct gaaactgctc
     1141 ctgtgtatac gatgacgccg ggagacgtgg acctcactct gaactggggg cgaatcagca
     1201 atgtgcttcc ggagtatcgg ggagaggatg gtgtcagagt ggggagaata tcctttaata
     1261 atatatcagc gatactgggg actgtggccg ttatactgaa ttgccatcat cagggggcgc
     1321 gttctgttcg cgccgtgaat gaagagagtc aaccagaatg tcagataact ggcgacaggc
     1381 ctgttataaa aataaacaat acattatggg aaagtaatac agctgcagcg tttctgaaca
     1441 gaaagtcaca gtttttatat acaacgggta aataaaggag ttaagcatga agaagatgtt
     1501 tatggcggtt ttatttgcat tagcttctgt taatgcaatg gcggcggatt gtgctaaagg
     1561 taaaattgag ttttccaagt ataatgagga tgacacattt acagtgaagg ttgacgggaa
     1621 agaatactgg accagtcgct ggaatctgca accgttactg caaagtgctc agttgacagg
     1681 aatgactgtc acaatcaaat ccagtacctg tgaatcaggc tccggatttg ctgaagtgca
     1741 gtttaataat gactgaggca taacctgatt cgtggtatgt gggtaacaag tgtaatctgt
     1801 gtcacaattc agtcagttga cagttgcctg tcagactgag catttgttaa aaaaatttcg
     1861 catggtgaat ccccctgtgt ggaggggcga ctggtgaaaa atccttgctt gtgattcatt
     1921 atcgacacgg gttcggtggt accaggccga actcaccggg aggcacccgg caccatgcag
     1981 tatacagaga ttaggcatat accaaggcct ctcatagcag gggccttttt acatgtaaaa
     2041 aagcccgagt gggttcgggc aattgcatga gatactcgtt ttaataatcg aaatcatttt
     2101 aaccaggatt cataaggctg cgcaactgcg cggccttttt cgtatttcgg gctgtagtct
     2161 tccttctgcc attgtcctgt aacttccgga cttcagcccg ctccttattt tactcacaat
     2221 attatcccgg ccgggaggat tcatggcatt taaacactat gatgttgtca gggcggcgtc
     2281 gccgtcagac cttgcgaaac gaataactca aaaactgaag gaagggtggc agccttatgg
     2341 tggtgcgctg atttcgacag ctggttatgg tgcggagttc atccagccag ttgtgagtga
     2401 ggggagcatc tcatcaccag aggagccagg caaccgtccg acgacctcag cgccttctgt
     2461 tgcgccagaa tattactatg tgatcgcgct tgctggtcag tccaatggta tgtcatacgg
     2521 tgagggactg ccattgccgg atacattcga cagccctgat ccacgtatta aacagttagc
     2581 gcgtcgcagt acggtgacac cgggcggtgc agtatgcaaa tataacgaca tcattccggc
     2641 ggaccattgt ctgcatgatg tgcaggacat gagccgtctt aaccatccga aagcggacct
     2701 gtcaaagggg cagtacggaa ccgtggggca ggggctgcat atcgccaaaa aa
//
LOCUS       DAZFKY010000327         2741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFKY010000327 DAZFKY010000000
VERSION     DAZFKY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..731
                     /gene="paa"
                     /locus_tag="ACHKCL_004809"
     CDS             <1..731
                     /gene="paa"
                     /locus_tag="ACHKCL_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO5765600.1"
                     /translation="FLSSAAYADINLYGPGGPHTALLDAAKLYAEKTGIIVNVHYGPQ
                     NKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSILLVKKGNPKNI
                     RSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRKNIILYAPNSG
                     TARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQDANDETRR
                     FAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1067..1222
                     /gene="hokD"
                     /locus_tag="ACHKCL_004810"
     CDS             1067..1222
                     /gene="hokD"
                     /locus_tag="ACHKCL_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5765601.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1443..1700
                     /locus_tag="ACHKCL_004811"
     CDS             1443..1700
                     /locus_tag="ACHKCL_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765602.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1770..2048
                     /locus_tag="ACHKCL_004812"
     CDS             1770..2048
                     /locus_tag="ACHKCL_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765603.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2050..>2741
                     /locus_tag="ACHKCL_004813"
     CDS             2050..>2741
                     /locus_tag="ACHKCL_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5765604.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          714 a          649 c          727 g          651 t
ORIGIN      
        1 tattcctgtc ttctgctgct tatgctgata tcaatctgta tggtcctggt ggcccgcata
       61 cagccttgct tgatgcagcc aaactttatg ccgaaaaaac aggtattata gtgaacgttc
      121 attacggccc acagaacaaa tggaatgaag atgccaaaaa aaatgcagat atcttgtttg
      181 gcgcatcaga acaatctgct ctggctatca ttcgggacca taaagacagc ttcagtgaaa
      241 aagatattca gcctctttat ctgcgaaaaa gtattttact ggtaaagaaa ggtaatccta
      301 aaaatatccg gagtattgac gacctgacca gacctgggat tggcgtaatt gttaatgatg
      361 gtggtggtac cagtaataca tcaggcactg gcgtctggga agatattgcc ggacgtaaag
      421 ggaatataga aactgtcgcc gcaatccgaa aaaatattat tttatatgcg cccaatagcg
      481 gaactgcacg taaggctctt gagaatcagc ctggagcaga tgtctggata acctgggctg
      541 actgggcagc cagtaatcca gaaattggtg atgtcgtgga aatagcgcca gactacgtga
      601 tatggcgtga tatgaacatt acagtacgtc aggatgcaaa tgatgaaacc cgtcgatttg
      661 cagaatggct acaaaccgat gaagcggcgc ctgcattcaa aaaatatggc tggaccagga
      721 aaggcacttg acatcctcgt ccttcaggac gtggattctt tttccggatg ccgcgccagc
      781 ggcatgtagg ggcagctcac aaaacggaaa aaattgtacg ctaagcctcg ccaggtgaac
      841 tgaattcatt ccgatatggg aattcccata tcgggcgaaa acggtttgct gtaacggcag
      901 agttaagtag gattgctgcg ggtgcttgag gctatctgcc tcgggcatga acaccaacgg
      961 cagatagata aaagccccac ccgactataa atcgaagtga ggcccctata tgctcgtcac
     1021 atatagattg cctcttacgg accgaaaggt caaggagaag caggctatga agcagcaaaa
     1081 ggcgatgtta atcgccctga tcgtcatctg tttaatcgtc atagtgacgg cactggtaac
     1141 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac
     1201 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1261 gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt
     1321 ttcaacgcgt ttgaagttcc ggacggcgcc ggaatagaat caaaaatact taagtagcgc
     1381 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1441 tgatgagaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1501 ttgagaaggg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1561 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttattttgc
     1621 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1681 tttctgcttt tgaagattag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1741 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1801 ataattcagc cgtctttgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact
     1861 ggttccggca cgccccatgc actgaagaac aggccgaatg gctgattcag aactaccgca
     1921 gacgtgggta tgagtttagg aaagccctca gcctcgatta tcgtcactgg ataatctacg
     1981 tcaggctgcc gtactccgag cgcccaccgc gtccgtcccg cacattccag caacgcatct
     2041 ggaggtaacg tgcgggtatt acttcgacct gttccggtac cggaacttgg ggtggtggtc
     2101 cttaaaccag gccgtgaatc catgcaggta ttccataacc ctcgagtgct tgtggagccg
     2161 gagccgaaaa gcatgcgcgg tctgccgtcc ggcatcgtcc ctgccgttcg ccagccgctg
     2221 gcggaggata aatcattact gccgtttttc agcaatgagc gtgtgattcg tgctgttggt
     2281 ggcgctggtg cactgtctga ctggctgttg cgtcatgtca aatcctgcca gtggcctcat
     2341 ggtgactatc atcacagtga aaccgttatt caccgttatg gtaccggcgc aatggtgttg
     2401 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2461 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2521 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2581 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aatccgctca
     2641 atgtaccgtg aaagcgacat cgtaccggga gagcagaccg ccaccatcat actgaagcag
     2701 cgcacaaaaa atcttgcgcc gctgcctcac gcccaccagc a
//
LOCUS       DAZFKY010000328         2700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFKY010000328 DAZFKY010000000
VERSION     DAZFKY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            25..579
                     /gene="ybeT"
                     /locus_tag="ACHKCL_004814"
     CDS             25..579
                     /gene="ybeT"
                     /locus_tag="ACHKCL_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5765605.1"
                     /translation="MNKKLMYIFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(589..2016)
                     /gene="djlB"
                     /locus_tag="ACHKCL_004815"
     CDS             complement(589..2016)
                     /gene="djlB"
                     /locus_tag="ACHKCL_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO5765606.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2013..2699)
                     /locus_tag="ACHKCL_004816"
     CDS             complement(2013..2699)
                     /locus_tag="ACHKCL_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5765607.1"
                     /translation="MLFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSSWGIDDRD
                     DLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGYTF
                     GRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQPLADYPTC
                     PETLKDMSDL"
BASE COUNT          777 a          638 c          598 g          687 t
ORIGIN      
        1 aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt cacaattttt
       61 atagttgcag caattacctg tattagccaa cccaagaaaa cgacgttgcg tgataaagcc
      121 atggtgaatt atgcctttga ttatttaagc tcaccgggca gtcttccatt caccacggca
      181 gccacggagc tttccgcgat tcatggtcac tcaacgtcgc aatatcgcct tggagaattt
      241 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
      301 caatcagcgg aacaggaaaa tccacgcgcg caaagtaaac tggggtggat ctacctcaaa
      361 ggtctggggg tcaaacccga cacccgtaaa gcaattctct ggtataagga agcagctgaa
      421 caagggtatg ctcatgctca atatacttta ggtttgatct acagaaatgg ctcaggtatt
      481 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
      541 aatgcggaaa gattacttgc cgggcttccc gcacattaac gtcgactgtc acccgaacag
      601 atggagtatt ttccctatca aaccagcaaa gataaaaata tagaaaataa ttttaatgat
      661 gcccagacag cctggcccac catcgtcaac gggcattggc tgcggttgcg gctccggttc
      721 aacagtttgc ttctcggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
      781 ttccagtgga caacgatttt ctgcatcaag acggcttacg atcggcatta atgattcttc
      841 tgtaggttgg ctggcctgca ctaattgttt ggcaactaga tataatggcc ataaggtatg
      901 ttctgtatca cgcgtgagtg agatccgcgg cttacatcct ttgtgcaaaa ccatctcgac
      961 cagtgaacgc cccagagcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
     1021 cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctggtcgt taaccagttc
     1081 acgagcttca atcatcatca ccactaatgg gtaataatcg actcgatgct gacggcacca
     1141 ttgcaacatt aaagcatccg cctgagtgga gggatactcg tgccagtagt cacaaagttc
     1201 tgccagcagg ctttcgcctt ctccacaata gacgcgttgc gcgtattcat aataacgcgc
     1261 atcttcatta tcaggttcag cttcccgcca cgaccgggcg taatccagga gttcggggat
     1321 tcccaggcga cctgcactat accagcggag taaatttctg tgcagttttg catcatcggg
     1381 gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
     1441 aatccgggcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg
     1501 cagaatgatc tggaagttaa aaacatttcc cttcgcaatg gcatataaaa aggattccag
     1561 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
     1621 taacaccacg cactcgaagg ataaatgggc agtgttcatg gctattgtgc acagcgacca
     1681 gcgtaattca tcaatctcat tcatcgagca ataatttaat tgctgaatga atcgctgcca
     1741 ggcggagggc agaaaacgtt cactatcaga ggcaagtaac gcacgaaagg cgagcagaat
     1801 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
     1861 aatccggagc gcttcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
     1921 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga catcagtcgt
     1981 ttcctctatg ttgaggatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt
     2041 tccgggcatg tggggtagtc agccagtggt tgccagggta aggtcgcgta atatgcatcg
     2101 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
     2161 aagaattcac gtaataaagg cagattatca tcttcgggag actgccaata ttgccgacca
     2221 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
     2281 atacgcgact ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
     2341 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
     2401 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga
     2461 tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc tgccaaccgc
     2521 gcggcatgac cgttatcgat cattcggtga attgtgcgca gcaggtcatc acggtcatca
     2581 attccccagg aggaagaaag gtcaaaaaag cgtgtaccta aatacatttt gggtgatgaa
     2641 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
//
LOCUS       DAZFKY010000329         2691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFKY010000329 DAZFKY010000000
VERSION     DAZFKY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..933
                     /locus_tag="ACHKCL_004817"
     CDS             <1..933
                     /locus_tag="ACHKCL_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765608.1"
                     /translation="GKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIKQTK
                     DGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVF
                     LKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGM
                     LEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNG
                     IAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVT
                     LEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            1004..1603
                     /locus_tag="ACHKCL_004818"
     CDS             1004..1603
                     /locus_tag="ACHKCL_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5765609.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1663..>2691
                     /locus_tag="ACHKCL_004819"
     CDS             1663..>2691
                     /locus_tag="ACHKCL_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5765610.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     STEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGA"
BASE COUNT          724 a          617 c          833 g          517 t
ORIGIN      
        1 ggtaaagagc tactggaaaa agttgacctg acggaggata acgccagcag actggatgag
       61 ttttcgaaag agtggaagga tgctaacgat aagtggaatg ccatgtgggg cgtcaaaatt
      121 aagcagacca aagacggcaa acattatgtc gcgggtattg gcctcagcat ggaggacacg
      181 gaggaaggca agctgagcca gtttctggtt gccgccaatc gtatcgcgtt tattgacccg
      241 gcaaacggga atgaaacgcc gatgtttgtg gcgcagggca accagatatt catgaacgac
      301 gtgttcctga agcgcctgac ggcccccacc attaccagcg gtggaaatcc accggtattt
      361 tccctgacat cagacggaaa gctgaccgct aaaaatgcgg atatcagtgg cagtgtgaat
      421 gcgaactccg ggacgctcaa caacgtcacg gtaaatgaaa actgtacgat taagggcatg
      481 ctggaggcga ctcaggtcag aggtgacttc gttaaagctg tatccaaatc atttccgaaa
      541 caggctggta cgtggggtaa cacggaaaca ccaaacggga cggttacagt caccatcagc
      601 gatgatcata actttgaccg tcaaatcatt attccgccca ttatctttaa cggaatagcg
      661 tatagcgatc cgggaagtgg taataacccg ggaggtacaa gatacacggg ttatggtttt
      721 gaagttcgca aaaacggtgt attaatcgca tccagagaaa ctaaaggggc cattcccggt
      781 agctacagtg cggttattga tatgccgagt ggcaggggaa gcgtcactct ggagtttaag
      841 gttttccata aaggcaatca gcgggcaggt aatatcaccg actgtacggt gattgtgacc
      901 aaaaaagcgg cttccggcat cagtatccgt tgaaatattt ataaccctaa taacgggcgc
      961 cagaaatggc gcctttttta ttgcagaaaa tcgggaggta attatgcgta aagtttgtgc
     1021 agcaattttg tccgcagcca tttgtctggc cgtatccggt gtgcctgcat gggcgtctga
     1081 gcatcagtcc acgctgagcg caggatatct tcatgccagt acggacgctc ccggcagcga
     1141 tgatctgaac gggattaacg tgaaataccg ttatgagttt acggacacac tggggctgat
     1201 tacgtccttc agttatgcca acgccgaaga tgagcaaaaa acgcattaca gcgatacccg
     1261 ctggcatgag gattacgtgc gtaaccgctg gttcagcgtg atggcagggc cgtctgtgcg
     1321 tgtgaatgaa tggttcagtg cgtatgcgat ggcgggtgtg gcttacagcc gtgtgtcgac
     1381 tttttccggg gattatttcc gcgtaactga caacaagagg aaaacgcacg acgtgctgac
     1441 cggaagtgat gacgctcgct acagcaacac atctctggcg tggggggctg gcgtgcagtt
     1501 taacccgacc gaatcagtgg ccgttgatgt cgcttatgaa ggctccggca gcggtgactg
     1561 gcgcactgac ggtttcatcg tgggtgtcgg ttataaattt tgattagtaa cacagcgtta
     1621 tgacagcccg ccggttcagg cgggcttttt tgtggagtgg gtatggcagc agtacaaata
     1681 tcaggcgtgc tgaaagatgg tgcgggaaaa ccaatacaga actgcaccat tcaactgaaa
     1741 gccagacgta acagcaccac ggtggtggtg aacacggtgg cctctgaaaa tccggatgag
     1801 gcagggcgtt acagcatgga cgtcgagtat ggtcagtaca gcgtcactct gttggtggag
     1861 ggattcccgc catcacatgc cgggaccatc tccgtgtatg aagattctca accgggtacg
     1921 ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc actgcgtcgt
     1981 tttgaacaga tggtggaaga agcagcgcgt cacgcagagg aggcgaagaa gaatgccgga
     2041 gaagcagaga cgtccgcgag gaatgccggc atatcagcca gtaaggcgga agcgagcgcc
     2101 gcaaatgctg atacttcagc agaggatgca tcggagtcag cccggcaggc ggcagaaagt
     2161 gcagcctctg caaagaagtc agaggaagcg tcctcgtcct cagcctctga ggccgctcaa
     2221 aaagccagtg agtcattaca aagtgcaaca gatgccgagt tgtcaaaaaa gacggcagaa
     2281 agtgcagccg gtaatgcagc cagggatgca acgacctcaa cagaaaaagc ccgggaatca
     2341 gcagaaagcg cacagtcagc ggaacaaagc agaatagcgg cggaagacgc cgtaaacaga
     2401 attcccaccg tggtggggcc tcccggacca aagggggaac cgggtcccgc gggtcctcag
     2461 gggccgaagg gagataaagg agagcgtgga gacaccggtc cggcaggggc aaccggtgaa
     2521 cggggaccgg gaggagatac aggtccggca ggtccgcagg ggccgaaagg cgacagggga
     2581 gagcggggag agaccggtct gacaggaagt acaggtccac aggggccaaa gggagatacc
     2641 ggggcaacag gtccggcagg accgcaggga ccgaaagggg aaacaggtgc g
//
LOCUS       DAZFKY010000330         2691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFKY010000330 DAZFKY010000000
VERSION     DAZFKY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..1207)
                     /locus_tag="ACHKCL_004820"
     CDS             complement(26..1207)
                     /locus_tag="ACHKCL_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5765611.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            1694..2128
                     /locus_tag="ACHKCL_004821"
                     /pseudo
     CDS             1694..2128
                     /locus_tag="ACHKCL_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2170..>2691)
                     /locus_tag="ACHKCL_004822"
     CDS             complement(2170..>2691)
                     /locus_tag="ACHKCL_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5765612.1"
                     /translation="EYPSYLKINFADFSLIKGLISWANHCAEYIEIFDESIAFTCLSA
                     FSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSRLYLSESLLKRKLKEEG
                     VSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQ
                     RSSESKILTHQGI"
BASE COUNT          788 a          621 c          546 g          736 t
ORIGIN      
        1 tttccgaaag tggttgagac acaaattaac tcctttttag tctttcaaat atcaaattac
       61 tgtaccagtc catcatagac ttccttccac taaaatattg agcatggtta taagtccccc
      121 taatagaatt tttatctaca tgagccaact ggatttcaat ccaagcactc tcaaaacctt
      181 gctcatgcag gattgtagaa agggtatgac gaaaaccatg tccagtgact tttcctccat
      241 acccaatacg cttaatggct tgatttatac tcgcttcgct cattggccta ttcggatcgt
      301 tccgccctgg aaaaacataa cgatagttcc ctgtcatgat cttgagttca ttgagtaaat
      361 ctaccgcttg agatgataag ggcacaagat gtggcctacg cattttcatc ctttcagcag
      421 gaatttccca aatagcgtta tccagatcaa attcttgcca taatgccgcg cgtaattcga
      481 tggttctcac acccgtaatc atcagtaatt tcgtggctat ctggacaagc ttactcccgg
      541 agtaaccctc taaggcacgt agaaaatcag gtatctcatc agcttttagg aatgggaaat
      601 gattggattg gtgtacttcg agagcgctgg agagatcagc cgcaggattg tactccgccc
      661 tacccgttgc aattgcgtag cgaaacactt cggagcaacg ctgccgcact ttgcgcattt
      721 tctccaacgc accacgtttc tcaattttac gcagaacgtt cagcagctct agcggtttaa
      781 tctctcccac aggccttgtt ccgacataag gaaaaatgtc gttcttaaac gcttccatga
      841 tgtctgatgc atatcccgcc gaccatttag cagatttaag ttgatgccac tctctggcta
      901 tcttttcgaa ggcgttctct gactctgttt gcatagctag cttttgctct tttcgaacct
      961 cactagggtt ctttccttct gccacaagtt ttcgagcttc atcacgacgg gaacgagcat
     1021 cggcaagggt gatcgttggg taaacaccaa gcgagatcat tttgggtttg ccggcatagc
     1081 gatagcggaa ccgccaactc ttgcttccat taggttctat aagtaatgac aaccctagcc
     1141 catctccaaa tgtataggct ttagcttcag gcttagcgcg gcgaatctgc atatcgttta
     1201 aaggcatgtg tataggaatc cagaccgaac aggaacatat acacaatcct atacacattc
     1261 agtaccggat tctactggat ggttacggac tactatggac ttaaaagaat aaaaaatgtt
     1321 ataaatcaga gattttatgg actaatacgg acgtttgagg aagttgagat ggtgccgata
     1381 ataggagtcg aacctacgac cttcgcatta cgaattataa gaatccgctt ctaattcaaa
     1441 gcattacccc atcaacactg cgctcacacg tcccaccaca tcaaaacatg taaagccttg
     1501 caagccattg cgaggcctta tgtgtctcag ttttgtccca ccttgtatta cgacttgcat
     1561 agccaatgaa gataaacgtg acgacaaacg gcgcagcagt cttcttttct ttcacgactt
     1621 ctccagcatg catacctttc tgccatgact gaagtgaatg cctgttatga gcaaggattg
     1681 gcccagatga atcgccctgg actgttgcga tcgtgaagcc atagactggg ccgcaagcac
     1741 gggaggttac gataaagcga cagtgcagga tgtcatgtta ggagcgatag aaaaacgctt
     1801 tggcgataaa gtaccggaac agtccatcca gtggctgaca gataacggtt cagcgtatag
     1861 agcgcatgaa acacggcagt tcgccagaga gttgaatctg gagccctgca caacggcaat
     1921 cagcagtcct cagagtaatg gaattgcaga aaggttcgtg aaaacgatga aggaagacta
     1981 catcgcgttc atgccgaaac cgaatgtaag aacggcattg cataatcttg cagtggcgat
     2041 cgaacattac aatgaaaacc atccgcacag tgcgttgggt tatcgctctc cgcgagaata
     2101 tcgacgtcag cgggtaacgt taacttaaga taccccccct gtctggaaat aagggggcaa
     2161 gaacaatgat cagattccct ggtgagtaag aattttactt tctgaactcc tctgagagta
     2221 ttgacttgga gaaacaccaa aataacgtct aaatacataa gtaaagtatg agacactggc
     2281 ataaccacag accttagcta ctttactaat aggataacaa cgagtgctga gtaaatattc
     2341 agccatttgc atcctctcat caagtatgat cttactgaat gataccccct cttctttcaa
     2401 tttcctcttt agtaaacttt cgctgagata taatcttgat gatatatcct taagacgcca
     2461 tggtgcagat aaatccgtat gaataatcgt tttaacttta gcccctgtgc ttttcaaaca
     2521 tccaaacaga aatactccaa attgtttttc agaagaaaat gcagagagac atgtaaaagc
     2581 aatagactca tcaaaaattt ctatgtattc agcacagtga ttagcccaac taattaatcc
     2641 tttaattaaa ctgaagtcag caaagtttat ttttaaataa gatggatatt c
//
LOCUS       DAZFKY010000331         2659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFKY010000331 DAZFKY010000000
VERSION     DAZFKY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..288
                     /locus_tag="ACHKCL_004823"
     CDS             <1..288
                     /locus_tag="ACHKCL_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5765613.1"
                     /translation="VLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGF
                     VSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(381..740)
                     /locus_tag="ACHKCL_004824"
     CDS             complement(381..740)
                     /locus_tag="ACHKCL_004824"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5765614.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1426..1716
                     /locus_tag="ACHKCL_004825"
     CDS             1426..1716
                     /locus_tag="ACHKCL_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5765615.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1740..1991
                     /locus_tag="ACHKCL_004826"
     CDS             1740..1991
                     /locus_tag="ACHKCL_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5765616.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2039..>2659)
                     /gene="traT"
                     /locus_tag="ACHKCL_004827"
     CDS             complement(2039..>2659)
                     /gene="traT"
                     /locus_tag="ACHKCL_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO5765617.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          737 a          536 c          570 g          816 t
ORIGIN      
        1 gttttattat atgtctctga ccatggagaa tctctgggag aaaatggatt atacttacat
       61 ggaacaccat ataatgttgc tccagctgag caaacgcatg tgcctcttat tacctggatg
      121 tctccggggt tcgtttcctc taaaaaaatt gatttgaatt gtttgtcaga acatgctcta
      181 aacagaacag taagtcatga taatatattt tcatcgttac tggggttatg gaatgtaaat
      241 acttcagttt ataataaaga agatgatatt atatcctcct gcagatagat actgacattt
      301 ttctgaggat aagaggtgaa tacctcttat ccttaagtaa atattaacca ggtaactgtg
      361 aataatttag atgttgggcg ttatgaaagt aataaaactc cccatataat gatagcaact
      421 aaaatcgtaa tcaacactgc ggctgaaccc atatcttttg ctctgcctga taattcgtga
      481 tgttctgaac caattctgtc aacaactgct tcaattgcac tgttaattat ttcaacaata
      541 attaccatca ttgccgaacc aatgagtaat actcgagtaa cagagtcaac atccagccaa
      601 aaggatatta tcagtgctga cagaaccaga atgcactcct gacgaaaggc tgcttcatag
      661 cgccatgccg cacataagcc tttccatgaa taacccgctg ctttaataat tctggtaaca
      721 ccagtggtcg tattagccat aaatacctct tatttaatta tatgtctgaa tatgcattat
      781 aaaatatcag ttaacattaa caggattttg taataagcca catttattct ttcgggaatt
      841 aaaaaacata acgaaatttt gcctcattct gcatttcatt gaaatagtat gtttctcatc
      901 gaaagcaagt ttgtcaatat aaatttgtaa cagaaatgac tttccgggga taaacaaaac
      961 tgttcatggc aatggctttc tctgtcgcca acatggatac attgttccgg ataaagcact
     1021 gatgtcattg actgacgcag actatcaggc catctgatgc tttcttttcc ttcaccggta
     1081 ttgatgatat gccttatgaa gggggactta ttcctgtaac ccaatcacag gcactgtgat
     1141 tactggttta tgattgatgc tgatttgagc agatatgcac agcagttgtg gattcagggg
     1201 atattttctg ccagatattc ataccagatt gatgacctct ttgctcagat gctcttcgtt
     1261 ctgagccagc caggattttg tggccacata cattgttcgc cgatggggaa tcgagacagg
     1321 cgattggctg tgatgcgatt ttgccgctaa ctgaggatga gtccgcccct cccggggaat
     1381 tcctgtaagt taagccgtac ctgttgatta gcactaaact gggtaatgaa tttatctgcc
     1441 agtatgaatg atgccgggga cggttttgaa ctcctgatgt cgggtaatat gcaggatttt
     1501 aatatttgtt gtcgtcggga taatcattat cctgaacata aactgatacc tttatcagag
     1561 ttatttgacg ttaattatgg actgaatctt gagctgaata aactggagaa agattcttca
     1621 ggaattaatt ttgtttcaag aacgtccaaa aataatggcg tctccgccag ggttaaatta
     1681 aaagatggta ttagtccatg ccagcagctg tgttaactgt agcagcagga ggttctgtta
     1741 tggaatcgtt tgtacaggat tcaccatttt atagtggaag agatctttat tggttacgtc
     1801 ctaaagttga actgactctg gaagagaaat tgtactactg ctcttgtata agaagaaaca
     1861 gacacaaata cagctacggg cgtcaggcaa accgaacgct gaagaattta ctggtaccgt
     1921 cattagattc tgttccggca tgggtatatg gtgtgacagg gaaaataata tctgaattgt
     1981 ctgaacgttg agactaagag ggccggaggc caggtgaatg gccaccggga tatttatatc
     2041 agagtatatt ggcgattgat tttgccagct ggtcttccag atgaggttta gcttcttcaa
     2101 acttcagatt aacctggttg gcactggata caacccgggt ctggtattta tgctggttgc
     2161 cggtttctgt gcttgtctga attttggagc ctgatgttcc ctgacgcaaa gctgcaacat
     2221 tatccgttct gacgctggtt ctggttcgct cggcaatctg aacatctgta atcattgtgt
     2281 aatttatatc ttccaccatt gcatccgcgg ccatgccaat cagaccggta gccagtccaa
     2341 caccgagagt ggtacctgcc gacccggtat tgtacgctgt gattccacct ccaagagcag
     2401 ctccgactgc tgcaccttca taaccgcgac tcagccatcc ctgagattca cgcagatcca
     2461 ttttgtctgc tttgagaacg ttagcctgga tccagtagta agctttatcc ggggaagtca
     2521 tgatctgata acctctggct ttgactgcat cggcaatttt tccctgtagt ccactcatgt
     2581 ctttatccga cgtgtttttg atctgcagga aaacggtgcg ttcgctcgca ggttcaagcc
     2641 agatggttga ggtgtactg
//
LOCUS       DAZFKY010000332         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFKY010000332 DAZFKY010000000
VERSION     DAZFKY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            331..1308
                     /locus_tag="ACHKCL_004828"
                     /pseudo
     CDS             331..1308
                     /locus_tag="ACHKCL_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1828..>2655
                     /locus_tag="ACHKCL_004829"
     CDS             1828..>2655
                     /locus_tag="ACHKCL_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO5765618.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     "
BASE COUNT          881 a          546 c          561 g          667 t
ORIGIN      
        1 ttatatgaga acaccaataa gaataataat atttgatcac aactgaagat gctttttaca
       61 atgaagaatt attgttaaaa aacgctttgt tgaatcaacc gaactgttgc tgagttgcag
      121 gatcacatca cgtattttcc tgaaaacgca ggctgcttag tagcaaagca gaagttcaaa
      181 atcaccaact aaatagctgc gccgaatagt agatcaatga aagggaactc agcccggatt
      241 gtgcgatctg atcaatcgcc aaaaatcccc ccctaaccgg actgagcgat gccgatcata
      301 gcaccaattc cccgcgacga atgacgcctg atgcaaaaag caatctataa aactcacgat
      361 aaaaattatg cccgcaggct gaccgccatg ctgatgctgc accggagcgc tcgcgtcagt
      421 gacgttgcca gaacgctctg ctgcgcccgt tcctccgtca ggcgctggat taactggttc
      481 acgctgtcga gtgttgaagg tctgaaatca ttacccgcag gacgttcccg ccgctggccg
      541 tttgagcata tctgcacatt gtttcgtgaa ctgataaaac actctcccgg atattttggc
      601 tatcagcgtt cacgctggag tacagaactg ctggcaataa aaatcaacga gataaccgag
      661 tgccagttac atgccggaac cgttcgccgc tggttgccat tagtcgggct tgtgtggcgc
      721 agggctgcgc caaccctgcg tacccgtaac ccgcataaag cagcaattca caaagcactg
      781 gacgaatgta gtgcggagca tccggtcttt tatgaagatg aagtgtatat ctatcttaat
      841 cccaaaaccg gtgcggactg gcaacttcgc agacagcaaa aacatgtggt aacaccgggg
      901 cagaatgaaa aatactatct ggctggtgct ttacatagtg gaacgggcaa agtcagctat
      961 gtgggtggca acagcaaaag ctcagcctta tttatcagtc tgttgaaaca tctgaaattg
     1021 acataccgac gggataaacc tatcacgctc atagtggaca actacatcat ccataaaagt
     1081 cgggaaacac agcgctggct gaaagaaaat ccgaagttca gagtgattta tcagccagtt
     1141 tactcgccgt gggtgaatca tgttgagcgg ttatagcagg cgcttcacga tacgataacg
     1201 cgcaatcatc agtgccgttc aatgtggtaa ctgcttaaaa atgttcatta ttttatggaa
     1261 accgtgagcc cattccccgg tggtaaatat gggctgttaa aagtgtagcg gtattcggcg
     1321 cagctattta ggatggtcag gtcgcagaag ttctgtgcca ctacagtgcg tgtactaaac
     1381 aaaatgacga gggcacgaat gtcataatac gtgcgtatta cctgacagta gctaccaact
     1441 gagggggctt attccaaacc tgatttattc aacaaagctg gacttatcct tactctgaat
     1501 tattttccag acatatgcat catgtactgg ctcgcttggt tcaggaaata caggagcaaa
     1561 tctacagcta ttagatttat tcctatttgt atgcaaagtc gcattgcata atgcggagct
     1621 atgttacaga tacgcctgac gtgcgtagta aaattataaa gcaacatata tatacaagga
     1681 gaaaccgaga aaaataaatg agtcgtatgt tgggtagcat cgagataaat atttttttta
     1741 tcacattagc accttggtta agggtgttaa taataatatt ctctcactta ttaaaacata
     1801 aataattttt taagcggaaa tgtaattatg agactgccag agaaagttct ttttcctcct
     1861 gtcactagtg gcctgtcagg gcaggaaaaa caaaaaaaac cgaagagcat taccggattt
     1921 caggaaaatt atcaacgcaa tatcaggcca atcaaaacag catcagaagc ccgactacgc
     1981 ttctttgata aaatggtttc gaaagaaaac tctctggaag atgttgtttc tttaggtgaa
     2041 atgattcaga aggaaattta tgggcatgaa caaagaacat tttcaccagt tcatcataca
     2101 ggtaactgga aatcatcatt gttacacaac gcgctccttg gtctggcaaa tgtttataat
     2161 ggcttacggg aaacagaata ccctaacact ttcaacagag atggtataaa aagtactaac
     2221 tcttttagag ataacttatt gacaaaaaca agaactccca gagataattt tgaggaagga
     2281 ataaaacatc ctgaacatgc aacaatacca tatgacaacg acaatgaaag taataaattg
     2341 ctaaaagcag gaaagatagc tggtaacaat aacgagctgt tgatggaaat aaaaaaggaa
     2401 tcccaaagcg accatcaaat ccccctgtca gataagttcc tgaaaaggaa aaaacgatct
     2461 cctgtagctg aagataaagt tcaaaactcg ttaacaccag aaaattttgt tcagaaaatt
     2521 tcacttagtg atgagcttaa aacaaaatat gcaaatgaaa ttctagagat aaaaagaata
     2581 atgggagaat acaatctttt accggataaa aacagtcgta atggtctaaa acttctacag
     2641 aagcaagctg attta
//
LOCUS       DAZFKY010000333         2624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFKY010000333 DAZFKY010000000
VERSION     DAZFKY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..947
                     /locus_tag="ACHKCL_004830"
     CDS             <1..947
                     /locus_tag="ACHKCL_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5765619.1"
                     /translation="TKQRNLFSKKMTLCISLFFVFGGAIVAHILSSHYESYNLHIAEL
                     TNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLF
                     MSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFN
                     ELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLA
                     RVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQGN
                     ALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            1045..>2624
                     /locus_tag="ACHKCL_004831"
     CDS             1045..>2624
                     /locus_tag="ACHKCL_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5765620.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGL
                     LQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLI
                     HNNVTLVQGYLYAKPMKAT"
BASE COUNT          800 a          454 c          550 g          820 t
ORIGIN      
        1 acacaaaaca gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg
       61 tttttggggg ggcgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac
      121 acattgccga gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca
      181 taatgatttt catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct
      241 atgatatctg gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc
      301 tgttatttat gagtaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag
      361 tcagtaagtt aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa
      421 caaagaacat tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta
      481 atgaactgac agttcaatca gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg
      541 atatcgacca cttcaaaaaa gtcaacgaca cctgggggca tccggttggc gatcaggtga
      601 taaaaacagt ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg
      661 tcggcggcga agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt
      721 tagcggaaag gattcgggaa aatgttgagc gtttaactgg cgataatcct gaatacgcta
      781 taccacaaaa agtgacgatt agtattggcg ctgttgtgac tcagggaaat gcgttaaacc
      841 cgaatgagat ctatcgactg gctgataacg cactatacga ggcgaaagag accgggcgta
      901 ataaggtggt tgtgagggat gtggtgaatt tttgtgagtc accataaagc ggcattttag
      961 cgggtgattt tggtaagcgc taaatagcta ttcgcagaca tgcgtaacaa ttttcagaaa
     1021 tatcatagta ctatatgatc ttaaatgctt atcattgatc agtctgctat cacccgcttt
     1081 tactggcaat tgtactataa tttcttaggt tgcatcaccc tgatgcgtgg ggaaacatgt
     1141 atggtattca acaaaaaaat gtttgtactc attataatac caggcattct gggggtgtta
     1201 ctgtcgtttg ctatgtcagt atttcaaatg aatcgagata cgacaattac cgccggtatt
     1261 ctgttaaaac agcttgataa tgttacgcaa attgtcaaac atacgacaaa gcttacttct
     1321 attctggtga tgaaaccctg caaagatatc ctggaacaat tgattgctaa cggagcattg
     1381 accccctatg ttcgaacaac gggtcttata gaaaataatt tccaaatatg ttcttcagtt
     1441 agcggtttca aaaaaatgaa tgttaacgat gtttacggta cgagctttca caataaaaat
     1501 aaagaatcac gcatagtatc aattagcggt acaagttttg taccaggtaa aacagccata
     1561 gtgttcctta tgccaatagg gaatgatatg acagctttta gtattgtaga gtcacgatat
     1621 atctatgatc tgatggatgt tcttgatgat gagaatgatg attcattttc tcttcgcttt
     1681 actgaagggc ctgcaattat tagtggtgta aataataatg acaggttata tatgttaaaa
     1741 aaagatttta attctgcgat cagtcaggct agtcttacag ttactacgcc gatgatttca
     1801 ttatatccct atgtcattag taatgtattt tacattcttc cattatctat tcttctttct
     1861 tttatcctgt acttcttatg gcagcgctgg atatctcgga aaatgtccct ggcagaagaa
     1921 attaagaaag gaatgtcatc cggagaattt tctgtgcatt atcaaccagt atgtgacacg
     1981 acaaccaaag cgtgtctggg ggttgaggcg ttaatgcgtt ggcaacgcga agatggtaaa
     2041 aatatatctc cggtagtttt tatccgcgct gcagaagagg aaaatttaat tataccgtta
     2101 actaagcatc tctttgaact gattattcag gatgtacaaa gctggaaagt gaaaaagccg
     2161 tttcaccttg ggataaatat tgccgctgaa catcttgcac atcctgattt tgttgctgat
     2221 gttttacata taaaaaatgc aatatctgat aaatttaaca ttgtccttga gattaccgaa
     2281 cgcaatctgg ttgaagatac tgatcatgca ttacagaaaa taaatgaatt aaggagccat
     2341 ggctgtgaat ttgcagttga tgattttggc acgggatatt gttcccttgg ccttctgcaa
     2401 aagttgtctg ttgattatct taaaatagat aaaagcttta tagacacgct cactacagcg
     2461 gaagacgaaa cgcctgtttt agatattatt attaagctga gtaatcgact caatttgata
     2521 acgattgcag aaggtgtcag tacctctcac caggcggaat atcttattca taataacgtg
     2581 acattagttc agggatactt atatgcaaaa ccaatgaaag cgac
//
LOCUS       DAZFKY010000334         2584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFKY010000334 DAZFKY010000000
VERSION     DAZFKY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..1318
                     /locus_tag="ACHKCL_004832"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ACHKCL_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1329..1484
                     /locus_tag="ACHKCL_004833"
                     /pseudo
     CDS             1329..1484
                     /locus_tag="ACHKCL_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(1861..>2584)
                     /locus_tag="ACHKCL_004834"
     CDS             complement(1861..>2584)
                     /locus_tag="ACHKCL_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5765621.1"
                     /translation="VYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKS
                     EGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHS
                     IKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMA
                     QQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVE
                     PLAIIDRTFGMKENISMVNASK"
BASE COUNT          735 a          580 c          604 g          665 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aacataaaat
     1381 tttatctgca tggcaaagag ataccagcag aaagaatatt agatacacca gagtggaaag
     1441 actaccgtcc aaaatactcc ggttccacat ataaatattc ttaatgatag caaaaaatat
     1501 attttcgata taatcaatgt tatgattaaa gagtatttca tcagggcagg taaaaacaga
     1561 gtaaatcagc aaaagaagct gatcttcagc gatactgaca ctaactgacg gtttaagcgg
     1621 tcgtatgaag cagcagcttt ccgacggact gccatgcgga tcgtttacct tttgggctat
     1681 tccgcccgtc atcaagcggc tcacgagtac tgagtttatc aggatgatat tgctgacaat
     1741 ggtaattcgt tgaccgatgt gtacttcact acatatcggt caacaccgta ctgccgtgca
     1801 acatctccaa gatgtagcat cgtttttacc agaggaggca ggacatagat tgtaaaaaaa
     1861 ctatttcgat gcatttacca ttgagatatt ttccttcata ccaaaagttc tatcaattat
     1921 cgcaagtggt tcaacaggtg cggtcttatc tataacccaa ccactctctg taacacttcc
     1981 tcgtgtgata tattttgcct ttactttaac aactattaga taatgctcac gactaaaccg
     2041 atcagcaacg ctaagatccg atgtgtattc aggcaatatt cttccaatac gtacctgctg
     2101 ttgtgccata tattcatctg gtgcttttac atttggattt gaatcaggaa gccctccagc
     2161 agttttatat tcaacgatat tttcagcttg aataccggtt gtgccgcgac acaatatgat
     2221 gtctgaatcg gacagttcta tgtgcccctc cttaattttc gaataaattt gtttaatact
     2281 atgttctgga gttggtacta tttcagaaga taaagcaggc aacgtgtctt caagcaccgg
     2341 agaatcatcg tatatatcca acaaatcctc atcataccca atatctcctt ctcttaactg
     2401 agaatacggt ttatctatgt atctaataac tcccccacca tgaagacctt ctgatttata
     2461 aaatgtacca tcagcgtcac gaaagaaata aacattaacc tgtttgttat ttatagaata
     2521 cctacctgaa tataattccg aatttggctc cttagttaaa tctactgatt cccccttgta
     2581 gact
//
LOCUS       DAZFKY010000335         2560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFKY010000335 DAZFKY010000000
VERSION     DAZFKY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            348..491
                     /locus_tag="ACHKCL_004835"
     CDS             348..491
                     /locus_tag="ACHKCL_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5765622.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            504..713
                     /gene="cspE"
                     /locus_tag="ACHKCL_004836"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             504..713
                     /gene="cspE"
                     /locus_tag="ACHKCL_004836"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5765623.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            879..1688
                     /gene="rlmA"
                     /locus_tag="ACHKCL_004837"
     CDS             879..1688
                     /gene="rlmA"
                     /locus_tag="ACHKCL_004837"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5765624.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1685..2251)
                     /gene="mntP"
                     /locus_tag="ACHKCL_004838"
     CDS             complement(1685..2251)
                     /gene="mntP"
                     /locus_tag="ACHKCL_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5765625.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          687 a          600 c          615 g          658 t
ORIGIN      
        1 gagagccgtt aactgcctgt tttatgagaa aaagtgatat aactttttat tcattgcata
       61 gcagaaaatg tgatattgca cgcactatgt aataacttct cccactgacc tggaacaact
      121 gaacttattg aactatgtta gaaactacgt cagtttaagt atctgcggaa ctgacaaggt
      181 taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga
      241 cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg
      301 ttgtatgtga taacagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt
      361 tcagtaaaga agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt
      421 atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga
      481 aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg
      541 agtctaaagg ttttggcttc attactcctg ctgatggcag caaagacgtg ttcgtacact
      601 tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg
      661 aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc
      721 cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc
      781 tttttactat gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc
      841 gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc tgtccacttt
      901 gccatcagcc tcttacgcgt gaaaaaaaca gctatatctg tccccaacga catcagtttg
      961 atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc
     1021 cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc
     1081 agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttaatgaa aaggccacgg
     1141 cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc
     1201 ccgaaatcac cacgtttggt ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac
     1261 gctatccgca ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca
     1321 gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag
     1381 tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga
     1441 aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt
     1501 ttacattaca gaagagtgat gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg
     1561 tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaatac
     1621 tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt
     1681 cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc agcaccagcc
     1741 cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc
     1801 ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga
     1861 cctgaaggaa agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg
     1921 tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc
     1981 cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc
     2041 agtgattcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg
     2101 gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg
     2161 gtttatggag ggtggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa
     2221 acgcaagaag aacagtagca gtgatattca tgacaacgtc ctgaccgggg tatccataaa
     2281 acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat gggttttcgc
     2341 gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa gtcttcagtc
     2401 cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa tctggaaatt
     2461 ggctactccc caaatgaggg cgcaacctta acatattttg agaatataaa acaactgttg
     2521 gaatatatta ttttattgct ttgttgataa cgattttcat
//
LOCUS       DAZFKY010000336         2548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFKY010000336 DAZFKY010000000
VERSION     DAZFKY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            343..534
                     /locus_tag="ACHKCL_004839"
     CDS             343..534
                     /locus_tag="ACHKCL_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5765626.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(587..820)
                     /locus_tag="ACHKCL_004840"
     CDS             complement(587..820)
                     /locus_tag="ACHKCL_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765627.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(916..1539)
                     /locus_tag="ACHKCL_004841"
     CDS             complement(916..1539)
                     /locus_tag="ACHKCL_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5765628.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1628..2137)
                     /locus_tag="ACHKCL_004842"
     CDS             complement(1628..2137)
                     /locus_tag="ACHKCL_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765629.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          767 a          631 c          525 g          625 t
ORIGIN      
        1 acaaatatcg caaaaacagc acattacaaa atttataacc ataattactg gaaaagtgca
       61 gaaaattatt ccactgaatg cttttaatac agttggttag cttaaaaaat agcaattttt
      121 gatttcagtc attgaaagtt gcaatgtttg acgattcaac agcatttaag tgagtttcag
      181 gatatgggaa ggacatgaaa ggaggagcgc aataaaatac acaggaagaa ggggaaaatg
      241 tttcgtaaaa acagaaaatt aacagcgcac atcaggaaac gcagtctcct gattcagtgc
      301 taccacatga aaatttagtt aactgttacc gtactaaaat ttatgtcaga agatgttcca
      361 ttaccgaaag tcaatcagcg atacaaagac gatcatggtg cgcttgtcac cctgaccagt
      421 gttgaggaaa cccgggtggt gtttatgcag gacggctatc cgcatccctg catgcgaccg
      481 atgtacaact ttctgggaaa attcaaaccc gaaccccggg aagaaacaga ataaaacgaa
      541 atccctgccg gcaatcacac tacctgatag cagcataatc cacttattaa ttctcatccc
      601 cggagacaag cccagaaaac cactccgggt gttcactgta tgccgtgttc agcgcatccc
      661 tggaaacgtt tatataaaag ctgtcctgca atttgcgggt aacaacttct gccaggcgat
      721 tccggttatc caccagtccg gcataacctc tctctgccag ccactcattc agttcatgtt
      781 ccagagacag attgataata tcataatcgg accttgccat tcccgtttcc ttagcacatg
      841 ttttcaggca cggggataaa gtataaagaa tgtcatactg ctgctgccag agcaatattc
      901 cccctgatgg ttgtatcaga gatggtatgc cggtactttg cgggttgttc ccgtcaggtt
      961 atcgtgcacc tccacggtcg tggtcaccac cggcattccg gcctttctca gcctcagaac
     1021 atcagccgca atacgctgac tgccaaacct gaacaggccg tccagtgcag tcaccagtaa
     1081 acccgcctcc agtgcatact tcagccgttc acggggcgct ttcacttccc gggcaacctg
     1141 ctgatatggc aatgatgtgt tttcattccc aaccacgcga cgaatacggt gagacagatg
     1201 ataccggtat gtatccggca caccagaaag gctggccttc aggctgtaca cgcagccaaa
     1261 ccgtttatcg ttaaacacca catttttctg gctgatgccc cattcttcac gcagcgcggc
     1321 aatcaattgc ggtgtacggg taagcagcaa gcgaaaaggc agttcaaaac tggtgacgta
     1381 atccacattc agcagtgcaa tacgcaaccg ttcttctggc ccggcctctg tctgccggca
     1441 ctcctccaga acatcctgcc actgccggcg aagacggggg gattcgttcc gttctgtaaa
     1501 acagtattta tccgccagat agtcaattcg tgtacgcata ctgaaaggaa ttccgtataa
     1561 agattcagca gacaaaactt tatcagccag taaaaattaa cggaagcatt gatattcctc
     1621 ccgacaatca ccggatgatt gttgcaataa cctgcgacat aaaggactga acagccgttt
     1681 ttaacgccac gtattgccct gatgaaccag gccgggccac ccgaaaacat ctttccaccc
     1741 ggcattgtcc gccagcactt atcaccgacc aggtctgttg cagtaaatcc accagttgcg
     1801 cacattcttc atcagccatg acgccgactc gcggatggca aatactcgtc tggctgagca
     1861 gcaacagaca tcgtatctgt gctccttcat gttgttcccg caaccagcgt aataccaccg
     1921 gcgctgatgg gctgaccgtg tgattatgct ccgtaatacg ttcgtgtacc cgtcggtgaa
     1981 aggaactaca aaatgtctga atctgttgac catagattcc ttctgccgaa tagagcgtga
     2041 agtgaaatgc caacagattt ggccagtggt ccaccgcctg ctgaacaaaa cgccggattt
     2101 cccccggctc tgaaattaag gcttcggtta tttgcacgaa atgttccttt tcaacccctg
     2161 ttgaattttg tcaagccggt gcagacacag taaagcgcgc atcaacacct gaaaggtacg
     2221 atacaaacac aagttacaaa caataagaac ggaaggacaa ttaatcagtg aggaataata
     2281 actaaattaa ccaaaagaat gttactgaca atactaccaa tacttaaata gcaatatatt
     2341 aattaatgta agaacagatg cccggtagga catatacaca atacatccac aaacacactc
     2401 ttttcattaa atcaatcacc tgattaaacc atgaaagtgt gatgtaagtc aaaattactg
     2461 atactatcaa ttgataatga ttatctctat cattatcgtg aaattcgaga atgaaaggag
     2521 aagtagtcat tataagcctt ttataaat
//
LOCUS       DAZFKY010000337         2508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFKY010000337 DAZFKY010000000
VERSION     DAZFKY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2123
                     /locus_tag="ACHKCL_004843"
     CDS             <1..2123
                     /locus_tag="ACHKCL_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5765630.1"
                     /translation="YILSTLSLCISGIVSTATATSSETKISNEETLVVTTNRSASNLW
                     ESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLIL
                     IDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGD
                     KLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYR
                     NNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFY
                     DTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQ
                     AVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQ
                     STVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDH
                     EMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNN
                     FELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRA
                     KTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQ
                     ANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDK
                     RYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          733 a          558 c          563 g          654 t
ORIGIN      
        1 tttatatcct ttccactctg tcattatgca tttctgggat agtttctact gcaaccgcaa
       61 cttcttcaga aacaaaaatc agcaacgaag agacgctcgt cgtgaccacg aatcgttcgg
      121 caagcaacct ttgggaaagc ccggcgacta tacaggttat tgaccaacaa acattgcaga
      181 actccaccaa tgcctccata gccgataatt tgcaggacat ccccggagta gagataacag
      241 acaactcctt ggcaggccgt aaacaaatcc gcattcgtgg cgaagcatcc tcccgtgttt
      301 taattctcat tgatggtcag gaggtaactt atcagcgcgc cggagataat tatggtgtgg
      361 gactgttgat agatgagtct gcgctggagc gtgttgaggt agtgaaaggt ccatattccg
      421 tactgtacgg ttcacaggca attggcggta ttgttaactt catcaccaaa aagggaggtg
      481 acaaacttgc atctggagtt gtgaaagctg tttataattc cgcaacagca ggctgggaag
      541 aatcaatcgc ggtccagggg agcatcggtg gatttgatta tcgcatcaac ggtagttatt
      601 ctgatcaggg caatcgtgat acgccggatg gacgtctgcc gaataccaac tatcgtaaca
      661 atagtcaggg tgtatggttg ggttataact ccggaaacca tcgttttggc ctctcgcttg
      721 atcgctacag actcgcgaca caaacttact atgaggatcc agacggaagt tatgaggcat
      781 ttagtgtcaa aatacctaaa cttgaacgag agaaagttgg ggtattctat gacacagacg
      841 tggacggtga ctatctaaaa aaaattcatt tcgacgcgta tgagcagacc atccagcgcc
      901 aatttgccaa cgaagtaaaa acgacacagc ctgttcccag tccgatgatt caggctctga
      961 ccgttcataa caagactgac acccatgata agcaatacac tcaggcggtc acattgcaga
     1021 gtcacttttc gctgcctgct aataatgaac ttgttaccgg tgcacagtac aaacaagata
     1081 gggtcagcca aaggtccggt ggcatgacct caagcaaatc tctgaccggc ttcattaata
     1141 aggaaacacg aactcgctcc tattatgagt cagagcaaag tacagtctca ctattcgcac
     1201 aaaatgactg gcaattcgcc gatcactgga catggacaat gggagttcgc caatactggc
     1261 tttcttcaaa gttgacgcgt ggtgacggag tatcatatac cgcaggcatt ataagcgata
     1321 cctctcttgc cagagagtct gcgagtgatc acgaaatggt aacatctaca agcctgcgct
     1381 attcaggttt cgataacctg gagttacgcg ctgcgttcgc gcaaggctac gtatttccca
     1441 cactctccca gctttttatg cagacatctg cgggcggcag tgtcacatac ggaaatcctg
     1501 atcttaaggc tgaacactcc aataactttg aattaggtgc acgatataat ggtaatcagt
     1561 ggctgattga cagcgcagtt tactactcag aagctaaaga ttatattgca agtctgatct
     1621 gtgatggcag tatagtttgc aatggtaaca ccaactcctc ccgtagtagc tactattatt
     1681 atgacaatat tgatcgggca aaaacatggg gactggaaat aagcgcggaa tataatggct
     1741 gggttttctc gccatatatc agtggcaatt taattcgtcg gcaatatgaa acttcaacat
     1801 taaaaacaac taatacaggt gaaccagcga taaacggacg tatagggctg aaacatactc
     1861 ttgtgatggg tcaggccaac ataatctctg atgtttttat tcgtgctgcc tctagtgcaa
     1921 aagatgacag taacggtacc gaaacaaatg ttccgggctg ggccactctc aactttgcag
     1981 taaatacaga attcggtaac gaggatcagt accggattaa cctggcactc aataacctga
     2041 cagacaaacg ctaccgtaca gcacatgaaa ctattcctgc agcaggtttt aatgcagcta
     2101 taggttttgt atggaatttc tgatgacaaa aacaatcgtt atggaattct ttgtcctgat
     2161 ctgcccgctt tgcgggcagg ctaacgaacg tattgtgttt gttgaaggtt tttcgacgtt
     2221 gttttccatc tgtgggtgaa tatttattct tcccgccagc atgactctat actcattgct
     2281 acgacaatga cttataaagc tggttactga acctatacct ttgtaattca aaagtgatat
     2341 cagttcacaa cgcaatacta tttttagatt atttactctc acccagatac tgaatcaatg
     2401 gataatacct tttgatttat cccgacgcgg aagaaggtgg tgaaccgccc cggaaatcct
     2461 ggagactaaa ctccctgaga aagaggtaaa caggatgact aaaaatac
//
LOCUS       DAZFKY010000338         2499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFKY010000338 DAZFKY010000000
VERSION     DAZFKY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..79)
                     /locus_tag="ACHKCL_004844"
     CDS             complement(<1..79)
                     /locus_tag="ACHKCL_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765631.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFD"
     gene            complement(63..1814)
                     /locus_tag="ACHKCL_004845"
                     /pseudo
     CDS             complement(63..1814)
                     /locus_tag="ACHKCL_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1826..>2499)
                     /locus_tag="ACHKCL_004846"
     CDS             complement(1826..>2499)
                     /locus_tag="ACHKCL_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5765632.1"
                     /translation="PELLNILDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQ
                     GRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCEVPDELIDFTFEWKGL
                     KKLCVAVSFRSIIAEQKKEPKMTVRYYISSADLTAGKFATAIRNHWHVENKLHWRLDV
                     VMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLA
                     GSGLS"
BASE COUNT          503 a          738 c          564 g          694 t
ORIGIN      
        1 tatcaaaccc aaggtaaaca acaaataaac aaagggtaat taaaaaaatg acaagtttgc
       61 ctctaataat ttgaagcatc tgaactccct tgctttgctt ttattggatt atctgctttt
      121 ataccatcta acccgaagag ttgcccaatg ttatattttt caatacatgc atcgctacat
      181 ttggcagaaa aatcgccaca agcaataccg acagtgttat ttttaagatc catgattctt
      241 tctttagctg gctgaccttg tctatcccca gccgcctcat gatttttacc tatcacgtca
      301 gctattgttg atccaaatgt cttagtcatt cgacacatca agtaacaata tctcattgca
      361 tcggcggccc cattatgagc tccgccaaca atagcatcca tatcataacc gaactggtaa
      421 gcactgttag taatgctgtt tgcaacaatc aatccaggta caccagatac ggcataaatt
      481 agtccacctg atgtcatatt ctcaatcgca tctaacccca ttgggtctat cacttgtatg
      541 ggattcaacg gatactgata catattccat ccacctctca acccaatcgg gtccggggtg
      601 atataccgcc cctgcaacgg atcgtagtac cggtgacgat tatagtacag ccccgactcc
      661 tcatcatgct gctggcccgg cagacggtac ggctggtgca ggtgatgcgg gttctcctca
      721 ttaagctggt tgccccattc atcatattcc gcgctccacg ccgtattgcc gtcttcgctg
      781 ataagcgcca gcggcagtcc ccggtgctca cagtggtaca gatgcagcgt gcgcgccggc
      841 gtgtattccg gctccacctg tttttccagt tgctccaccg tcagtccgca ctgcgcaagc
      901 cacgcccggc tttcactgct cacgcggtct gcccggattt cttcctccag cctgtccagc
      961 atcctcacca gttctgccgg gaacaccaca ccgtgaccgt cctcactccc ttcctgctgg
     1021 agtttctccg ccaggctgcg gcgctgcgct ttctcccgct cgccgttctc cgtttcgatg
     1081 cggatgagcg gtgtgaagct ccccggccgg tataccgtct ggatacgtgt ggtgccggtc
     1141 tgtaccgtcg tcagcctgtc gccgtcccac ccgtaccagg tcacctccgg tttacgcgac
     1201 agcgacatcc accccgtcag gtcacgctcc cgccgccaca cccgtttccc cgtccggcgg
     1261 cccagcgggt catagaggta gcggctctcg accagcggct cgccgtattg tatccgcgtg
     1321 tagaacacca ggcggtgctg gttgtcgtaa tggtagtggt gggtccgctc gtcgtgcatc
     1381 cggataaccc cttccggaat gcggtccgtc ttctccgtca gcctgccgta ttcatcgtag
     1441 cggtagacat agtgcgcatc ttccgcgatg cggttatccg gccacgccgt gagcgtgctg
     1501 tccggatgca gttccgggtc cggcagccgg tttcctgccg ggtccgttgc atacgggata
     1561 tcgatatcca ggttcgctgc cgtggtgtgg acgcccgtca gccttcccgt gtcgctgtaa
     1621 cggtactccc ggctctcctg cgggccgctg atgcggatta gctgtccgtt gtcgttccag
     1681 tcgtagtcac agtcaagctg cgggaggttc aggtgcctgc tctgtagctg ccctgtgagg
     1741 gtgtacgccg tgacctgttc gtaccccgct gtactgcccg ccccgccgaa gctgcgggcc
     1801 gtctcacggt gcagccaggg caagattacg aaagcccgct ccccgcaagg actgacgcca
     1861 gatagtttct gtccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
     1921 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
     1981 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc cagtgcagct
     2041 tattctccac gtgccagtgg tttcggattg ctgtggcgaa cttccctgcg gttaaatcag
     2101 cagaactgat ataatatctg accgtcattt ttggctcttt cttttgttct gctattatgg
     2161 accgaaagga gactgccacg cataatttct tcagcccttt ccattcaaac gtgaaatcaa
     2221 taagttcatc agggacttcg caaacaatat gaagacggat ttcttctctg ccgtgactct
     2281 tttcactaat tgcgtaactg tcatgctctg gattatttaa ttctttcagc ggaaattttt
     2341 cctcaaaggc tttatttagc cgcccctggt ttccttttac cgcgaataaa taatcacctc
     2401 cctgtttttg tatcttctct gcaatatctt tctggcaccc catcgcatca gttgtgatga
     2461 tttttccttt aatatccagt atgttaagaa gttcagggg
//
LOCUS       DAZFKY010000339         2490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFKY010000339 DAZFKY010000000
VERSION     DAZFKY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1472
                     /gene="escC"
                     /locus_tag="ACHKCL_004847"
     CDS             <1..1472
                     /gene="escC"
                     /locus_tag="ACHKCL_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO5765633.1"
                     /translation="LEKRLGKSEYFIITKSSPVRAILNDFAANYSIPVFISSSVNDDF
                     SGEIKNEKPVKVLEKLSKLYHLTWYYDENILYIYKTNEISRSIITPTYLDIDSLLKYL
                     SDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAKNKDVVKVF
                     KLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERSGNLFDNSVTI
                     SADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEISVSIIDVDANDLQQLGVNWSG
                     TLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNALQQNSKAKILSQPSIITLNNM
                     QAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDSSNSLTGKRRERVRLLLD
                     IQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESLLLGGFIQDKESSSKDGIP
                     LLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            1486..1941
                     /gene="cesD"
                     /locus_tag="ACHKCL_004848"
     CDS             1486..1941
                     /gene="cesD"
                     /locus_tag="ACHKCL_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO5765634.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2325..>2490)
                     /gene="grlA"
                     /locus_tag="ACHKCL_004849"
     CDS             complement(2325..>2490)
                     /gene="grlA"
                     /locus_tag="ACHKCL_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO5765635.1"
                     /translation="KRLEIFIYDVNLEAVPIESPGTTGPKRKTYRVGNGIVGQSNIWN
                     EMIMRRKKES"
BASE COUNT          832 a          473 c          451 g          734 t
ORIGIN      
        1 ctctggaaaa aagattaggc aagagcgaat attttattat tactaaaagt tctccagtac
       61 gtgcaatcct taatgacttt gctgctaact acagcattcc tgttttcatc agtagttccg
      121 tgaatgatga tttctctggc gaaataaaaa atgaaaaacc tgtaaaagta ctggaaaaac
      181 tgtccaaact atatcatctc acatggtatt atgatgagaa catcttgtat atttataaaa
      241 caaatgaaat atcaagaagt ataattacac caacatatct ggatattgac tcactgctaa
      301 aatatctgag tgatacaatc tcagtaaaca aaaactcttg caatgtgaga aaaattacga
      361 catttaactc gattgaagtt cggggtgttc ctgaatgtat aaaatatatt acatctttat
      421 cagaaagtct tgataaagag gcacagtcta aagccaaaaa taaagatgtt gttaaggtct
      481 ttaaactgaa ctatgcgtca gcgacagata taacatacaa ataccgtgac caaaatgttg
      541 tcgtcccagg tgttgtctcc attctgaaga caatggcaag taatggttca cttcccagta
      601 ccggtaaagg tgccgtcgag cgaagcggta acctgtttga taacagtgta acgatttccg
      661 ctgatccacg tcttaatgca gttgttgtaa aagaccgcga aataacgatg gatatctatc
      721 agcaattaat ttctgagctt gatattgaac aaaggcaaat tgaaatttct gtctcaatta
      781 ttgatgttga tgcaaacgat cttcagcagc tcggcgtaaa ctggtccggt acgttaaatg
      841 caggacaagg tacgattgcc tttaatagct ctaccgcaca ggctaatatc agctcttctg
      901 tgataagtaa tgccagtaat tttatgatta gggtgaatgc gttacaacag aactcgaaag
      961 ctaaaatact ctctcagcct tcaattataa ccttaaataa tatgcaggcg attcttgata
     1021 aaaacgtaac gttttataca aaagtctccg gagaaaaggt ggcgtctctg gaaagtatta
     1081 caagcggaac attgctgaga gtgacaccac gtatacttga tgacagctcg aatagcctta
     1141 ccggaaaacg tcgggaacga gttcgtttgt tacttgatat tcaggatggc aatcaatcga
     1201 ctaaccaaag caatgctcag gatgccagca gcaccttacc tgaagtacaa aattcagaaa
     1261 tgacaacaga agcgacatta agtgcaggag aaagtttatt acttggcgga tttatccaag
     1321 ataaagaaag ttcaagtaaa gatggtatac cgctgttaag cgacattcct gttattggta
     1381 gtttattctc cagtacggta aaacaaaaac atagtgttgt tcgactcttt ttaattaaag
     1441 caaccccgat aaaatctgca tctagcgaat aataaatgag gctttatgag caggaaattt
     1501 agctctctag aggatattta tgatttctac caggatggtg gcacattagc gtcattaaca
     1561 aatctgacac aacaagatct caatgacctt cattcttatg cctatacagc atatcaatct
     1621 ggtgatgtta taaccgcaag aaatctattc catttgctca catatctgga acactggaat
     1681 tatgactaca ccttatctct gggcttatgt catcagcgtt tatcaaatca tgaagatgca
     1741 caactgtgtt tcgcacgctg tgcaacttta gttatgcaag atcccagggc atcttattat
     1801 tctggaatta gctacttact cgtcggaaat aagaaaatgg ccaagaaagc ctttaaggct
     1861 tgtttaatgt ggtgtaatga aaaagaaaaa tacactacat ataaagaaaa tattaaaaaa
     1921 ttgttaggta atacagagta aaaactacca tcagctatac caatccccag gctgactggg
     1981 gattttctat cggaaataat aaagctgcta tagtgaagtg ctcccaaagt tgagcaccga
     2041 ccactggtag atttatctac aggtgtgggg ttattccatt aaccaggagt ttatgccctg
     2101 aactatcttc tgctgtattt aatatcattt ccgtttatga aacaatcagt atatcttaac
     2161 ttagcattag aaatctgatc tttccaatca tctatagctc agagtatatt tgtcaggaaa
     2221 tgaccttcaa actttagccg agaaaaaggc ttaccctgga aaacaaaacc cttaaatata
     2281 gctttatttt tattcttcta taaaatatac tcaaaaaatt acgtctaact ctcctttttc
     2341 cgcctcatga tcatttcgtt ccagatatta gactgtccca caataccatt accaactcgg
     2401 taggtttttc tttttggtcc ggttgttcca ggactttcta tcggaaccgc ctcaaggtta
     2461 acatcataaa tgaaaatctc aaggcgctta
//
LOCUS       DAZFKY010000340         2457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFKY010000340 DAZFKY010000000
VERSION     DAZFKY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..364
                     /locus_tag="ACHKCL_004850"
     CDS             41..364
                     /locus_tag="ACHKCL_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5765636.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            370..1506
                     /locus_tag="ACHKCL_004851"
     CDS             370..1506
                     /locus_tag="ACHKCL_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5765637.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWGHYLYLDH"
     gene            complement(1503..>2457)
                     /locus_tag="ACHKCL_004852"
     CDS             complement(1503..>2457)
                     /locus_tag="ACHKCL_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5765638.1"
                     /translation="YRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKVGLK
                     VFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITY
                     LSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFI
                     TQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYR
                     VKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIF
                     RKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          604 a          570 c          625 g          658 t
ORIGIN      
        1 tatgcgttaa ttgttctttt ttatattctc tgagggaaat atgttcctgg actattttgc
       61 actgggagtg cttatttttg tatttctggt gatattctat gggattatta ttttacatga
      121 tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca ttcatgttgc
      181 tggttgggtg agtcttttta cgctgcatgt tatctggcca tttttgtgga tttgggccac
      241 gctttaccgc ccggagcggg gatggggaat gcaaagccat gattcatccg ttatgcaact
      301 gcaacagcgc attgccgggc tggagaaaca gctcgccgac atcaaatcct cttctgccga
      361 gtaatactta tggatttact gattattttg acctatgtgg cttttgcatg ggcaatgttt
      421 aagatcttca aaattcccgt aaataaatgg accattccca cagcggccct gggtggaata
      481 tttattgtca gcggtctaat tctgttaatg aactataacc atccgtatac ctttaaagcg
      541 caaaaagcgg ttatttctat tcctgttgtc ccacaggtga caggcgtggt gatagaagtg
      601 acggataaga aaaatacgct gattaaaaaa ggtgaggtgc tatttcgact ggacccgacg
      661 cgttatcagg cgcgggtgga tcggctgatg gcggatatcg ttaccgcaga acataaacag
      721 cgggcgttgg gcgcagagtt agatgagatg gcggcgaata ctcagaaggc aaaggccacg
      781 cgggataaat tcgctaagga gtaccagcgt tacgcacgcg gcagccaggc gaaagtaaac
      841 ccgttttcag aacgcgatat tgatgtggca cggcaaaatt atctggcgca ggaagcctcc
      901 gtaaagtcat cggcggcgga acaaaaacag atccagagcc agctggatag cctggtgttg
      961 ggtgaacatt ctcaaatcgc cagcctgaaa gcgcagctcg cggaagcaaa atataacctt
     1021 gagcagacga tagtgcgtgc gccgagcgat ggttatgtga cccaagtgct gattcgtccg
     1081 ggtacctatg ccgcgtcgct gccgctacgt ccggtgatgg tgtttatacc cgatcagaaa
     1141 cgacaaatcg tggcgcagtt ccgtcagaac tccttgctgc gcctggctcc cggcgatgat
     1201 gcggaagtgg tatttaacgc tctgccaggc aaagtgttca gcggtaagct ggcagccatt
     1261 agtccagctg ttcccggcgg agcttatcag tcgaccggca ccttacagac gttaaacaca
     1321 gcgccgggtt cagatggcgt tatcgcgacc attgaactga atgagcacac tgatttgagc
     1381 gctttaccag acggtattta cgctcaggtg gcggtctact ctgatcattt cagccatgtc
     1441 tcggtgatgc gcaaagtgtt attacgcatg accagctggg ggcattacct ttatctcgat
     1501 cattaatctt cgctttccgc cgcttgttca ggccgcagtt gctgcgactg agcaagctgg
     1561 tggcgaattt cacgcagcat ttcatccagc acatactgga cccgccaggg ctggtattcc
     1621 cgtttacgga agatagccac cagatccagc caccattcat acgggtgctg aaagcagggc
     1681 accagcgtgc cgtcttgcag atcgctctgc acacttttgt ctggcgcaaa cacaatcccc
     1741 aggtgatttc gtgccagctc cagcgccgat tgcgtgttat cgcaaacgta atttcccttc
     1801 acgcgataat cccgcacctc tttactcccg gcgacgttga agcgccagat attcgcgtcg
     1861 tcgatcatca tcgagtcgat caaaatacag gagtgatgtt cgagttcgtc aggacggcta
     1921 ataggatgtt tctcaagata gcgctggctg gcataagcgg ttacggcata ttgggtaata
     1981 aaactggcaa ccagcgattc atcttttggt ggggcatagc tgattaaaac atcacaatca
     2041 tcaggaaatt cgacgccttc ataaaaggca ttacgctcaa gattgcaggt ttttagcgat
     2101 aaggtgatat caccgatatc tttaatttta tcgattacat gtttggataa ataggtaatt
     2161 atgccggttg gggcgtagat ggtgacccga ccacgtttct catgcttata atctgcaata
     2221 aaattattaa gctgctcgtt tctgtccagc atgtcgttga tgtacggtaa tagcgcggtg
     2281 ccaaaaagtg tgagcatcag ttgtcgggtg gttcggtcaa agacttttaa acccactttt
     2341 gattcaaaat cagcaagata tttgctgacg ttggcctgtg cgatgccaag tacagtcgcg
     2401 gcatggctga tattttcact ggcagcgatt accgagataa tttttaactc ccggtac
//
LOCUS       DAZFKY010000341         2437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFKY010000341 DAZFKY010000000
VERSION     DAZFKY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            170..867
                     /locus_tag="ACHKCL_004853"
     CDS             join(170..419,419..867)
                     /locus_tag="ACHKCL_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFO5765639.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            884..1144
                     /locus_tag="ACHKCL_004854"
                     /pseudo
     CDS             884..1144
                     /locus_tag="ACHKCL_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1286..2410)
                     /locus_tag="ACHKCL_004855"
     CDS             complement(1286..2410)
                     /locus_tag="ACHKCL_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO5765640.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          696 a          563 c          514 g          664 t
ORIGIN      
        1 agaattttaa acgacaagta agggaatata aatgaaaata acaaaaaata aaaatgcaac
       61 tataaaaaac agtcatcagt cggctgcttt tcaatgttta ccttatcatt ttctggtaat
      121 gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat
      181 ttccatcaga tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg gcaaaagcac
      241 tgccggacat cagcgctatc tctgctctcc ttgccgtaaa acatggcaac tacagttcac
      301 ttacaccgcc tctcagcccg gtaagcacca gaaaatcatt gatatggcca tgaatggcgt
      361 cggatgtcgc gccagtgcac gcattatggg cgttggcctc aacacggttt tacgtcactt
      421 aaaaaactca ggccgcagtc ggtaacctcg cgcatacaac cgggcagtga tgtgattgtc
      481 tgcgctgaaa tggacgaaca gtggggctac gtcggtgcta aatcacgtca gcgctggctg
      541 ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtcttcgg tgaacgcact
      601 ctggccacac tggagcgtct tctgagcctg ctgtcggcct ttgaggtcgt ggtatggatg
      661 acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgcacgt tatcagcaag
      721 cgttacactc agcgcattga gcgacataat ctgaatctga gacaacatct ggcaaggctg
      781 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
      841 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cctgagaata atacctcctg
      901 ggtaagtgag cagatacttt cagcgcctcc gaaagatgta aatattcgac ttggagtcgg
      961 ttctttagaa ggtacaaccg tctcacatgt tcagcgattg catcattcgt taatcgcagc
     1021 tggtttggaa agtaacctca ctgtctatac cggtggtcac gattatgcct ggtggcgcgg
     1081 agcaattatt gatgaattag caaattataa ttgcaggaag gtatcaaata ataactctgt
     1141 gtaaaatatc gttttatcgt cagataactc tttctgcaat attattatgg gagactatct
     1201 catttattta gtgatgtcct tcatttatgt gatgaaggac attatattac tcataacatt
     1261 tctcgttgaa aatttcagca atacgctaca attgtttccc gttgtttttt aattttctca
     1321 tcaaaacctc tgcaatctct gcaggactgg gttgttcagc catttcagcg gctacctgat
     1381 ctgaacagaa gcgtagtgaa tcatcataaa gaaggcgatt taccaaatca ctggtcagtc
     1441 catgcttgtc cggaataatc ccacacccac gcttcgcaac aatttctgca ttaacagagc
     1501 gatctgcacc ttcgccaaac acaatctgtg gtattccact atacagcgct gtcagggtat
     1561 tacctgcgcc accgtgatga ataaatccat cagctccatt aaggaataca cccataggta
     1621 tccagtcaac cagacgtaca tttgatggta gttttcgtaa accagtacga gcatttattg
     1681 caagttgcaa aatgatatca gcatcaactt catttgcaga atccataacc catgaaatca
     1741 gctccagacc atcaaccatt ggttttacag tgccaaggct gataagtaaa cgtttccgat
     1801 cagaatccct gtcccaccat gtttccttta cagcacctcc gttataagga atatatctca
     1861 ttgagataac cggttctcca gcatttttaa gaatgctcat acttggggga gcaacatcaa
     1921 tccatgctaa atcacatagt ggaccgctga ctccatgccg cctgtaagca ttgcttaaag
     1981 aacggtttaa catctgaata tgggcagatg tatgcgcgaa tccaacagcc agcatcactg
     2041 aaggaattct atatttagca gcaaccaatg ggcctgccgg atcaagaggg ggatagacta
     2101 ttaaatctgg cctccatttt cctgcaaaat cgatgaggtt atctgccatt tcatcgctaa
     2161 aaaaagagaa atgaccaata atattacttt ttttcctcaa ctcttcctga tggcgatatc
     2221 cagcctctga atctaaacct ggaactgcat caaaaacaac cagtccagct tctgctgctt
     2281 tattcgcgaa tttgccagcg ctactaatta ctacatcatg tccaattaca cgaaaggcct
     2341 gagccagaga aatcaatggg tataacaaac cgtacagcgg tgggccaata aaaagaatac
     2401 gttttcgcat taaaaaagtc cgtcttctgc ttacctg
//
LOCUS       DAZFKY010000342         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFKY010000342 DAZFKY010000000
VERSION     DAZFKY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            306..512
                     /locus_tag="ACHKCL_004856"
     CDS             306..512
                     /locus_tag="ACHKCL_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5765641.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(768..1040)
                     /locus_tag="ACHKCL_004857"
     CDS             complement(768..1040)
                     /locus_tag="ACHKCL_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765642.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1200..1733
                     /gene="rrrQ"
                     /locus_tag="ACHKCL_004858"
     CDS             1200..1733
                     /gene="rrrQ"
                     /locus_tag="ACHKCL_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5765643.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1954..2067
                     /locus_tag="ACHKCL_004859"
     CDS             1954..2067
                     /locus_tag="ACHKCL_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765644.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2069..2368
                     /locus_tag="ACHKCL_004860"
                     /pseudo
     CDS             2069..2368
                     /locus_tag="ACHKCL_004860"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2322..>2430
                     /locus_tag="ACHKCL_004861"
     CDS             2322..>2430
                     /locus_tag="ACHKCL_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO5765645.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDW"
BASE COUNT          624 a          492 c          669 g          645 t
ORIGIN      
        1 attaaggaaa aaaacagcca gcactgactt tcggtggaga ggtgctggct cagaaggata
       61 gttggatttc acatgatact tatgcctggc ggtatatttt ctgacagaca gtgacgggtg
      121 ttgtcaagat attgtgtcat ttataacctg aatcagggga ggccggaatg ttatctggca
      181 tttttagcag agcctgaatg ccataatcac ggctcccgga gttggccgtc agtgggtgac
      241 actggcggct tttttgtttt tctttacttt cattttctgt cggcggtgac ggagacatac
      301 atcagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg gggacggaat
      361 actggttact gcagctgctg gacaaagtct ctccgtccca gtgggtggca ataggagtgc
      421 tgggaagtct gctgtttggc ctgctgacgt atctgactaa cctgtatttc aaaatcagag
      481 aggaccgtcg taaggtggcg cggggagagt agtcgatgaa taaacaatac gaactggttg
      541 taaaatgaat atttctaact gaaaaaacgt tccatgaggt aagaaaaggt cacaggcaat
      601 caataacagg acgtgatgaa agacccttgc atttgtgcgc tttctcttta gatagcagca
      661 gatactgaaa atctgagttg tcggggagtc agggatacag ctgtgcaaga gttggtcatt
      721 gtgattccat tgaaatcctg tatgccatga agggcaggat tttatggcta cctgagcttt
      781 ggtgatagta agttgaaaat tcgcattttt tgctgacatg cgtaacgaga atcccataag
      841 cagggaggac ttaattcttc attaacccat gcgttgatat tatgtttcag ccgttgaagc
      901 atcagcggtg ttaatgttgt ggtaataata tccagcgttt tatgtgagat cttaccgtaa
      961 gggtctgcaa gaatgctgct tgttgcttcg ttattatctg ccatcagaag aagtaactct
     1021 gatttaacgt tttctgtcat tagttgtaaa aatcttctgc gcaaactttc tttactgttc
     1081 atttatatgg cttcatttgt tgtaatctgc tgcgtctcaa gggatatgtt tatgagagcg
     1141 accatgagtg ttggattata tacctaacat atcaagggat tagaaatcga taaatcccca
     1201 tgaacgaaaa aataaaatac ggcctgtcgg ctgccgttct ggcgctgatt ggtgcaggtg
     1261 cttctgcgcc tgaaatcctc gaccagtttc tggatgaaaa ggaaggtaac cacaccacgg
     1321 cataccgtga tggtgcgggg atctggacca tctgccgtgg tgccattctg gtggatggta
     1381 agcctgttat tcctggcatg aagctgtcaa aggaaaaatg cgaccgggtt aatgccatcg
     1441 aacgtgacaa ggcgctggca tgggtggaga aaaacatccg ggtgccgctg accgaacccc
     1501 agaaagcggg gatcgcgtca ttctgtccgt acaacattgg ccccggtaag tgcttcccgt
     1561 cgacgtttta taaacgaatt aatgcaggcg atcgaaaagg tgcctgtgag gcgattcgct
     1621 ggtggattaa ggacggtggc agagactgcc gtatccgttc aaataattgc tacggtcagg
     1681 tctcacggcg tgaccaggag agcgcgctgg cgtgctggga catcgacaga tagcagaata
     1741 ttttcctgaa aaatgacgtt ggccaacgcg ggtggataac acgaaatcct gaaaactggt
     1801 aaaacctaag tgaataaaag taaaaacccc gtttgttggc agcaagcggg gttttgtgtt
     1861 ttctgacctt gagtaaggca agggagaaat tatgggtagg gaggtacttt ccctgtgagg
     1921 aagtataaaa gattctttct gaggttgtcc attatgaaag gcattgaagt ggagacgcca
     1981 gccagtctgg atttaacaag agcggcagct tttgccattc gtattgtggc cattgctgtt
     2041 ctggtctggg caatccgttg gtggtgatat gaaccgtgtt ctgtgtgtgg ttatcattgt
     2101 cctgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgtg atcacgccat
     2161 catctacaaa gagcagcgcg ataaaaaagc cagtgagctg gagctggcga acgcgacaat
     2221 tactgatatg cagatacgcc agcgtgatgt cgctgcactt gatgccagat actcgaggga
     2281 attagccgat gcgagagctg aaaatgaaac tctgcgtgct gatgttgccg ctggtcgtaa
     2341 gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt gaagccaccg gcacctccgg
     2401 cgtggataat gcaaccggcc cccgactggc
//
LOCUS       DAZFKY010000343         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFKY010000343 DAZFKY010000000
VERSION     DAZFKY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..206)
                     /locus_tag="ACHKCL_004862"
     CDS             complement(<1..206)
                     /locus_tag="ACHKCL_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5765646.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDA"
     gene            complement(190..513)
                     /locus_tag="ACHKCL_004863"
     CDS             complement(190..513)
                     /locus_tag="ACHKCL_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5765647.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            638..1114
                     /gene="racR"
                     /locus_tag="ACHKCL_004864"
                     /gene_synonym="rarC"
     CDS             638..1114
                     /gene="racR"
                     /locus_tag="ACHKCL_004864"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO5765648.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1430..1582
                     /locus_tag="ACHKCL_004865"
     CDS             1430..1582
                     /locus_tag="ACHKCL_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5765649.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1697..2212)
                     /locus_tag="ACHKCL_004866"
     CDS             complement(1697..2212)
                     /locus_tag="ACHKCL_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765650.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            2336..2421
                     /locus_tag="ACHKCL_004867"
                     /pseudo
     CDS             2336..2421
                     /locus_tag="ACHKCL_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          697 a          573 c          518 g          633 t
ORIGIN      
        1 gcatcagggg tgtctttatc cagccaacgg aaaattttct gggtattacg agccagggct
       61 tccggatggc tgtcgtcgta cagttccggg aacgtcatcc ccagttcgaa ataagtccgg
      121 gctatttcag ctgcaggaac tttctcacca tcaggatatg cccaggcatt catcgccatg
      181 cggatgtgct catgtttgat tttcatgaat catttgcctc ttgatgcttc gggtatgatc
      241 gttttcgtca tttggttgct tcatcgacat attctgcgaa taacatgacg agcgtcgtaa
      301 gtatgtccaa tcaacatcag gacgaagttc ttcacacagg acaccacctt ttgttgctcg
      361 ttcaatcgca ggacatctct cagcaggcaa ctgacgtaca cctttgatcc attgatttac
      421 gcttggagga gatacaccta aaagcctagc cattgctgat tgcccaccga caacagcaca
      481 agctcgtttg aatgaatagt tatctttttt catcgaatga actccaaaaa acacacaata
      541 atattaggct tagcctaatg caattgtcaa taggctatgc ctaacgcttc gagagtaggc
      601 attgcctaat gcgatgcgca taggagacta ttaagcaatg cttagtggta aagacttagg
      661 ccgagcgata gagcaggcca ttaacaaaaa aattgcatca ggagccgtca aatcaaaggc
      721 ggaaatcgca cgtcatttca aagtccaacc accatcaatc catgactgga ttaagaaagg
      781 ttcgataagt aaagacaaac ttccagaact atggcgtttc ttttctgatg tggttggtcc
      841 agagcattgg gggcttaacg aataccccat accaacccca tccacttcag atacaaaaag
      901 tgaactttta gacataaaca gcctttatca agccgcctct gatgaaaaaa gagcaattgt
      961 ggctttcctc ttatctggaa atgctacgga gcctagttgg gttgatcatg acgttcgtgc
     1021 ctacattgcc gcaatggaaa tgaaggtagc taactatctg aaaaatcaag aatcaaaacg
     1081 gaaaagccag aacatcacca agacaggaac ttaaacttat atggtccgac gggaaattcc
     1141 tagttcccgt tagttaactc ctactacctc tcccacaaac catcacctat taggtcgcac
     1201 ccaaatcatt aggcgtagcc tattgacaag caattaggca tttcctatag ttttcccata
     1261 ccaacccatc ccgtcccaca caatacaggg caatacctag agttacccgg cagtggtcag
     1321 ggattaagta gccagcccga ggcgtaagaa catgacggca gggttcaact ttaactatgc
     1381 agcaggtttt tgttccgcta ccccggcgtt aaggggatat gaggtcagca tggatactct
     1441 tgatcttggc aacaacgaat ctctggtgtg cggcgtgttt cccaaccagg acggcacgtt
     1501 caccgcgatg acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgtcgctg
     1561 gttagcaaga aactctgact aatgaaatct ggtaattaag gaatcctcca cggggggaga
     1621 gtggtgcaca cgcgccggac acaagcaagc atccggcatg ctctttaaca atctggatat
     1681 ccataacagt aaaaatctac agattgccgt tcagttttct ggccaactct tcaatagatg
     1741 gaggtgatac gtaatccgga tttttattca taagaaactg attttcacag tacaggcacc
     1801 tgcttttatg aaaaaattca tattctctaa ccgggaatgg ctgaagtatc gatactatct
     1861 tttgtccaaa acattttgga caaagatgca tgattatgct gccgccgttc acggttacct
     1921 ccttcgagta cacaaaagtt cccgactcaa gttggctaag gatataacct tccgtctgag
     1981 cctcaaagtt ttcaaattct gcaattttag ctttgagaga agcatttatt tcttgataag
     2041 tgcccaccag ctcaacgaga gacacgcatt cgcgctgaat agatgcaagc tttgagttca
     2101 gttcaccaat agccgcattt acttcagctt gagtttttgc ctcgttcatt agttttgcaa
     2161 tctgggcggt ttcacgaata gccgtcattg ctgccgttaa ttcagcgatc acattcaata
     2221 ctcttattgt tgttggggat atccagatta acaagatcct tgttgttggg gaataacggg
     2281 gaccacctcg cctgacgtgg taaaaagcag gcacacaaca cgaaagcaca cggcgaggga
     2341 caccattaaa tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga
     2401 aatcccgtta gatgatgcac t
//
LOCUS       DAZFKY010000344         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFKY010000344 DAZFKY010000000
VERSION     DAZFKY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..132
                     /locus_tag="ACHKCL_004868"
     CDS             <1..132
                     /locus_tag="ACHKCL_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765651.1"
                     /translation="GWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
     gene            132..830
                     /locus_tag="ACHKCL_004869"
     CDS             132..830
                     /locus_tag="ACHKCL_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765652.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            841..1584
                     /locus_tag="ACHKCL_004870"
     CDS             841..1584
                     /locus_tag="ACHKCL_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5765653.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1581..2159
                     /locus_tag="ACHKCL_004871"
     CDS             1581..2159
                     /locus_tag="ACHKCL_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765654.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          513 a          586 c          818 g          504 t
ORIGIN      
        1 ggctggaagg catttttgtg gaagccaccc tatgcatacc ggcagataaa ggtgacctgt
       61 gccgggtggt ctgcgcgggt cgggatgttg cgcgttgagt tcagcgcgga gtttaagcag
      121 gtggtgaact gatgcaggat attcacgaag aaagtctgaa cgagtcggtt aaatcagagc
      181 agtcaccgcg ggtggtactc tgggaaatcg acctgacggc gcagggcggt gagcggtatt
      241 ttttctgcaa tgagctgaat gaaaaagggg aggcggttac ctggcagggg cgggaatatc
      301 aggcataccc gattgacggc agcggttttg agatgaacgg gaagggcagc agtgcccgcc
      361 cgtcgctgac ggtgtcgaat ctgttcggtc tggtcaccgg aatggcggag gacctgcaga
      421 gcctggtggg ggccacggtg gtccgccgcc gggtgtatgc ccgttttctg gatgcggtga
      481 attttgtggc gggcaatccg gaagcggacc cggagcagga gctgagcgac cgctgggtgg
      541 tggagcagat gtcagagctg acggccatga cagcctcgtt tgtgctggca acaccgacgg
      601 agacggacgg agcgctgttt cccggtcgca ttatgctggc gaatacctgt atgtgggatt
      661 accggggaga tgaatgcggg tataacggtc ctgcggtggc ggatgagttc gacaacccca
      721 ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgag atgcgcggca
      781 tggtggctaa ttttggcggt ttcctttcca tcaataaact ttcgcagtaa atcctgtttt
      841 atgacacaga ctgaatcagc gattctggtg catgcccggc ggtgtgcgcc tgcggagtcg
      901 tgcggcttcg tgataggcac cccggagggc gaacggtacc agccctgcgt gaatatctcc
      961 gcagagccgg aggcgtattt tcgtattgcg ccggaagact ggctgcaggc agagatgcag
     1021 ggggagattg tggcgctggt tcacagccac cctggtggtc tgccctggct gagcgaggcc
     1081 gaccggcggc tgcagataaa gagtgccctg ccctggtggc tggtctgccg gggggaaatt
     1141 cataaattcc gctgtgtgcc gcacctgacc gggcgtcgtt ttgagcacgg ggtgacggac
     1201 tgttacaccc tgttccggga tgcataccat ctggcgggga taacgctgcc ggattttgag
     1261 cgtgaggatg actggtggcg caacggtcag aacctttacc tggacaatat ggcggcgact
     1321 ggtttttacc gggtgcccct gtcctctgca caggcgggcg atatcctgct gtgctgcttt
     1381 ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact gcttcaccat
     1441 ctgcctgaac aactgagtaa acgggagagg tattctgaaa aatggcaacg acgaacgcat
     1501 tctgtctggc gtcaccgcca ctggcacgca tctgccttca cggggatttg caacgatttg
     1561 gccgccgcct cagcctgtac gtgaacacgg cagcggaagc catccgtgcc ctgtcgctgc
     1621 agatgccggg attccgccgt cagatgaacg aaggctggta ccagatacgt attcgcggtg
     1681 aggacacggc accggaggcg gtgtacgccc gtcttcacga acctctgggt gagggggcgg
     1741 tcatccatat tgtgccgcga ctggccggag ccggaaaggg cggactgcag attgtgctgg
     1801 gggcagcagc catcgtgggc tctttcttca ccgccggcgc aacgatggcg ttgtggggcg
     1861 cagccctgag tgccggaggg ctgactgcca ccacgatgct gttctcactg ggtgccagca
     1921 tgatactggg cggtgtggcc cagatgctgg caccgaaggc aaaaacaccg gattaccgcg
     1981 caacggataa cggtaaacag aacacgtatt tttcgtcact ggataacatg attgcccagg
     2041 ggaacccgat gccggtgcct tatggtgaaa tgctggttgg ttcacgacgg atatcccagg
     2101 acatcagcac ccgtgatgag ggcggagacg ggaaagtggt ggttatcggg cggggatgaa
     2161 aataaaaaaa tcccgcagag ttagcggagc tgcgggagag aacgatgaag attaacgtta
     2221 tggagttatt tttcaggcat caaaaaagta atgcagcgtc attattgcgg ctacaggcaa
     2281 ttgccggaaa tgtgaagagt ttcagaaatt ttattccgtc atgacacagg caccctccgg
     2341 ggtgcctgtt gttttctggc ataaacagat tcagacatca gacaggagag ggggacagag
     2401 tgggtaaagg gggcggcaag g
//
LOCUS       DAZFKY010000345         2393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFKY010000345 DAZFKY010000000
VERSION     DAZFKY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..185
                     /locus_tag="ACHKCL_004872"
     CDS             <1..185
                     /locus_tag="ACHKCL_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5765655.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHKCL_004873"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHKCL_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5765656.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHKCL_004874"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHKCL_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHKCL_004875"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHKCL_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          612 a          609 c          677 g          495 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag gga
//
LOCUS       DAZFKY010000346         2385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFKY010000346 DAZFKY010000000
VERSION     DAZFKY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            45..533
                     /gene="eco"
                     /locus_tag="ACHKCL_004876"
     CDS             45..533
                     /gene="eco"
                     /locus_tag="ACHKCL_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5765657.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(683..2329)
                     /gene="mqo"
                     /locus_tag="ACHKCL_004877"
     CDS             complement(683..2329)
                     /gene="mqo"
                     /locus_tag="ACHKCL_004877"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5765658.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          555 a          662 c          600 g          568 t
ORIGIN      
        1 gacatcatcg gtgcaaacct ataaatgaag aaggaaagca aaaaatgaag accattctac
       61 ctgcagtatt gtttgccgct ttcgccacca cttccgcctg ggcggcagag agcgtccagc
      121 cactggaaaa aatcgcgcct tatccacaag ctgaaaaagg catgaagcgt caggtgattc
      181 agttaacccc gcaagaagat gaatctaccc tgaaagtaga actgttaatc ggtcagacgc
      241 tggaagtcga ttgcaatttg catcgtctcg gcgggaagct ggaaagcaaa acgctggaag
      301 gctggggcta tgattattat gtctttgata aagttagctc gccggtttcc acgatgatgg
      361 cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc gtatctgggc gatactggaa
      421 tgctgcgtta caacagcaag ctgccgatcg tggtgtatac gccagacaat gtagatgtga
      481 agtaccgcgt ctggaaggcg gaagagaaaa ttgacaacgc ggtagttcgc taaactgccg
      541 tgaagtgcgg caccccgtag gttggataaa gcggtcacgc cgcatccgac atctaacgcc
      601 cgagccggtt gcctgatgcg acgctggcgc gtcttatcag catacgccac atccggcata
      661 ccatgccgga tgtggcacat cattacaacg caatatccgc cacttctttt tgaacgggtt
      721 gcggtttcaa ctgcggtttc ggcgtactat ctgctgcttg cggtttgtcg tatttcagcc
      781 ccagcacttc gctggtgtac tgcaactcgc gttctgttgc cgctacatca ccgttcagct
      841 tgcgtccata agatggaacg atcgctttca acgtagcctg ccattgcggg ctggaaacgc
      901 gatcgccaaa tactttttcc agcagattca acataatcgg cgcggcggtt gacgcccctg
      961 gcgatgcccc caggagcgcg gcaatggttc cttgctggtc actgaccact tcagtaccca
     1021 gacgcagtac accaccttta tcggcatcac gcttgataat ctgcacacgc tgccccgctt
     1081 gccacaaacg ccagtcctct tttttcgctt gcggatagta ctctttcaac gcttcaaaac
     1141 gatcctcttc actcaacatc acctgactca ccagatattt caccagatcg aaattatcca
     1201 gcccgacgtg catcatcggc atcacgttag aggtggtggt ggaactcatc agatcccaca
     1261 atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa tggcccaaac agcactacgc
     1321 gtttaccgtc cagaacgcgg gtatcgatat gcggaaccga catcggtggt gcgccaacgg
     1381 atgctttacc gtaaaccttc gccagatggt gattaaccac gtccgggttt tccgaaacaa
     1441 ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc ttccggaatc cccgattcct
     1501 gtaacagctt cagtgccgca ccgcccgcgc cgataaagac aaatttcgca cggatgttct
     1561 gtgcagtgcc atttttcaga tcggcaacgg taacggtcca ggtgttatcg tcattacgct
     1621 ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt agatttcttc tgcaaggaag
     1681 caattaactg gcgggtgatc tcgccgtagt tcacatcggt accaatttcc gtacgcgtgg
     1741 ctgccacttt ctgttgcgga tcgcgccctt ccatcactaa cggtgcccac tctttgatct
     1801 gcgcgtgatc ttcagagtaa cgcataccgc gaaacagcga gctttgttgc aacgcggcgt
     1861 aacgggcgcg caggaaattg acgttatcct cgccccagac aaagctcata tgcggaacgg
     1921 tattgataaa tgaacgcgga gtacgcagca cgccgcgctc gacctggtgc gcccagaact
     1981 ggcgggaaat ctgaaatgct tcgttaatgg cgactgcttt ttcaatactg atgctgccat
     2041 cggcgttttg cggggtgtag ttcagttcca tcagtgcaga atgcccggtt ccggcgttat
     2101 tccagccgtt cgaactctcc tgcgcgacac cctccaggcg ctccaccatg gtcatcgacc
     2161 attcaggctc cagctcgcgt aaataggtcc ccaacgtggc gctcataatg ccgccgccaa
     2221 tcaacagtac atcagtttcc tgctcctcgg acgctttcgc ttttgccgcc atcgaaacgg
     2281 cattaagccc cacggccatc gagaagagca tggcagtcac ttttttcatc ttgttaatgc
     2341 cttactttta gtcgctttat tgcaggtgag atttgcgcgg catca
//
LOCUS       DAZFKY010000347         2381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFKY010000347 DAZFKY010000000
VERSION     DAZFKY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..453
                     /locus_tag="ACHKCL_004878"
     CDS             <1..453
                     /locus_tag="ACHKCL_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765659.1"
                     /translation="NLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEAD
                     PEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGY
                     HGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            complement(470..724)
                     /locus_tag="ACHKCL_004879"
     CDS             complement(470..724)
                     /locus_tag="ACHKCL_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5765660.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            834..944
                     /locus_tag="ACHKCL_004880"
     CDS             834..944
                     /locus_tag="ACHKCL_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5765661.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1247..2125
                     /locus_tag="ACHKCL_004881"
     CDS             1247..2125
                     /locus_tag="ACHKCL_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFO5765662.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2179..>2381
                     /locus_tag="ACHKCL_004882"
     CDS             2179..>2381
                     /locus_tag="ACHKCL_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5765663.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHS"
BASE COUNT          654 a          493 c          631 g          603 t
ORIGIN      
        1 aatctgttcg gtctggtcac cggaatggcg gaggacctgc agagcctggt gggggccacg
       61 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcgggcaat
      121 ccggaagcgg acccggagca ggagctgaga gaccgctggg tggtggagca gatgtcagag
      181 ctgacggcca tgacagcctc gtttgtgctg gcaacaccga cggagacgga cggagcgctg
      241 tttcccggtc gcatcatgct ggcgaacacc tgtatgtggg attaccgggg agatgaatgc
      301 ggttatcacg gtcctgcggt ggcggatgag ttcgacaacc ccaccacgga tatccgtaag
      361 gacagatgca gcaagtgcat gcgcgggtgt gagatgcgcg gcatggtggc taattttggc
      421 ggtttccttt ccattaataa actttcgcag taatggatta tgcccaccat caggtgggtt
      481 ttttatcttc gccgtacatg ctgctgagag tcttaagaag agcctcccta aatttttcag
      541 attcttgttg agcaaaacct tctattgatc ttggtgattt ttcttcatca atagcagctt
      601 gcaagatcat aactatttct gaatttaatg agcgtccgtt tttcgatgct cttatggtca
      661 ggtcgcgctt gaggctatct ggcattctta cgctatatgg ggctatgtct ctaaccttcg
      721 tcatacttac accgtgataa tcacattgat atcacagtgt attcaaaaaa actttgactt
      781 gatatagtca cagtgatacg tttgtgattc ataataactc acgcggtggc gatatggaaa
      841 aagaagttag cagaattttg gtgagaatac ctcagtcgct aaaagatgcg attacaggaa
      901 aggccaaaga agaatgccgg tcgtttaact cggaagtgat gtgattgctg gacagcctga
      961 agagagaggg gataacggta tgagtaaaga atgttgtttt ttgcggcatt agcgaatcag
     1021 acgctgatca aacatacatt tactctaaag aaacaggtcg gatgctgtgt agtgactgcg
     1081 tgtttgacat cataagatac aagtatcttg gatgttctgc cagcattagc aatataggtg
     1141 aagtgtacga agggaaagat ataactgata gagcagaaag ttgaagcccc aactgctgta
     1201 acagtcaggg cttcgttatc aacaaatcgg cttaggaaat attgacatga aaagtatagc
     1261 aaaggcacaa aacgatttca ctatcttcaa attcggcgac agtgaaatcc gcgtcatcaa
     1321 caagtgcggt gagccgtggt ttgtagctaa agatgtttgt gatgctttag ctttgactaa
     1381 ctcacgcaag gcgcttactg cacttgatga cgatgaaaag ggagtaactt taagttacac
     1441 ccttggtggt gagcagaatc taagcattgt gagcgaatca ggtatgtata cattggttct
     1501 gcgctgccgc gatgcagtca ataaaggttc agtcccgcac aaattccgca agtgggtaac
     1561 agcagaagtt ctgccttcaa ttcgcaaaca tggcgagtat gtaaaaggaa agaaaaccac
     1621 tgttgaggaa agaacaccgc tacgcgatgc agtaaacatg ctggtaggaa agaaaggact
     1681 tcgcaatgac gatgcataca atatggttca tcagcgtttt ggtattgaca gcattgatga
     1741 gctttcaatt gaacaaatcc cgcttgccgt agagtacatc cacagggtag tgcttgaagg
     1801 tgaattcatt ggcaaacaag agaagagcac caacgagctt tccgcaaaag aagcaaacag
     1861 ccttgtatgg ctatgggatt atgccaaccg ctcacaggca ttattccgcg aactgtatcc
     1921 ggcgctaaaa caaattcaat cgaactattc cggcagatgc tacgactacg gtcatgagtt
     1981 ctcgtatgtt attggaatgg cgagagacgt tttaattaat cacacacgag atgttgatat
     2041 caatgagcca gacggaccaa cgaatctttc cgcatggatg agacttaaga ataaagaatt
     2101 acctccttca ccgcatcact actaacagat tgccaacgaa atgacccagc ttcggctggg
     2161 tttttatcag gagttctcat gctctatagc aatatattgg cgcacgcccg gcgatgtgca
     2221 ccagcggagt cgtgcggctt cgtggtgaga acgccggaag gggaaagata ttttccctgc
     2281 gtgaatatct ccggtgagcc ggaagattat ttccggatgt cgccggagga ctggctgcgg
     2341 gcagaaatgc agggggagat tgtggcactg gtccacagcc a
//
LOCUS       DAZFKY010000348         2372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFKY010000348 DAZFKY010000000
VERSION     DAZFKY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1991
                     /locus_tag="ACHKCL_004883"
     CDS             <1..1991
                     /locus_tag="ACHKCL_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765664.1"
                     /translation="LSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLCRRLFRCVSVRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRL
                     TSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPA
                     APSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLGSALYWI
                     AASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKTHLAQEL
                     WTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNS
                     MWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQ
                     GDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVT
                     INENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIV
                     IPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHM
                     TLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
     gene            2059..>2372
                     /locus_tag="ACHKCL_004884"
     CDS             2059..>2372
                     /locus_tag="ACHKCL_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5765665.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSV"
BASE COUNT          567 a          603 c          694 g          508 t
ORIGIN      
        1 tcctgtccat cgatgccgcc agccgcaccc tgacgctgga ccgtgaggtg acactgccgg
       61 agaccggtgc cgccacggtg aacctgatta acggcagcgg taagccggtg agtgtggaca
      121 tcaccgcaca ccccgcgccg gaccggatac aggtcagtac cctgcctgat ggtgtggaga
      181 catacggggt gtggggactc tccctgccgt cactgtgccg tcgcctgttc cgctgtgtct
      241 ccgtccggga aaacacggac ggcacctttg ccatcacggc ggtgcagcac gtaccggaaa
      301 aagaagccat cgtggataac ggtgcccgct ttgagccgca gtcaggttcc ctgaacagcg
      361 tcatcccacc ggcagtacag cacctgacgg tggaggtgag tgcagctgac ggccagtatc
      421 tggcgcaggc taaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc
      481 tgaccagtgg taagggaacg gatgccagac tggtgaccac cgccatcacc gcagacacgg
      541 agcaccgttt cagcggcctg ccgctcgggg aatacaccct gacggtgcgg gcgataaaca
      601 gctatggcca gcagggtgaa cctgccacca ccaccttccg gattgccgca ccggcagcac
      661 cgtcgcggat tgagctgacg ccgggctatt ttcagataac cgccacgccg catcttgccg
      721 tttatgaccc gacggtacag tttgagttct ggttctcgga aaagcggatt gcggatatca
      781 ggcaggttga aaccgcagcc cgctatcttg gctcggcgct gtactggata gctgccagta
      841 tcaatatcaa accgggccat gattattatt tttatatccg cagtgtgaat actgttggca
      901 aatcggcatt cgtggaggct gtcggtcggg cgagcgatga tgcggaaggt tacctggatt
      961 ttttcaaagg agaaatcggg aaaacacatc tggcccagga gctgtggacg cagattgata
     1021 acggtcagct tgcgccggac ctggctgaaa tcaggacgtc cattacgaat gtcagcaatg
     1081 aaatcacgca gaccgtcaat aaaaaactgg aaaatcagag tgcggcaatc cagcagatac
     1141 agaaagttca ggttgataca aataataacc tgaacagcat gtgggccgtg aaactgcagc
     1201 agatgcagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag aatacgccag
     1261 caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga
     1321 atggcaacac aaagccgatg tttgttggtc agggcgatca gatatttatg aatgaagtgt
     1381 tcctgaaata tctgacggct cccaccatta ccagcggcgg taatcctccg gcattttccc
     1441 tgacaccgga cgggcggctg acggcgaaaa atgccgatat cagcggtaac gtgaatgcga
     1501 actccgggac gctcaacaac gtcacgatta acgagaactg tcgggttctg ggaaaattgt
     1561 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaagctttc ccccgggact
     1621 cccgtgcacc ggagcggtgg ccatcaggaa ccattaccgt cagggtttat gacgatcagc
     1681 cgtttgaccg gcagattgtt attccggcgg tggcattcag cggcgctaaa catgagaaag
     1741 agcatactga tatttactcc tcatgccgtc tgatagtgcg gaaaaacggt gctgaaattt
     1801 ataaccgtac cgcgctggat aatacgctga tttacagtgg tgttattgat atgcctgccg
     1861 gtcacggtca catgacactg gagttttcgg tgtcagcatg gctggtaaat aactggtatc
     1921 ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact gcaggcatca
     1981 cgattagctg aattttataa cccagatacg ggcgccagaa atggcgcctt ttttattgca
     2041 gaaaagcgag aggtaattat gcgtaaatta tgtgctgtta ttttgtccgc agtagtctgg
     2101 caggtcgccg ctgctacgcc agcgagtgca gcagaacatc agtccacgct gagcgcgggg
     2161 tatctccatg cctcgacgaa cgttcccggt agtgatgatc tgaacgggat taacgtgaaa
     2221 taccgttatg agtttatgga cgcgctgggg ctgattacgt ccttcagtta tgccaatgct
     2281 gaggatgagc aaaaaacgcg ctacagcgat acccgctggc atgaagattc cgtgcgtaac
     2341 cgctggttca gcgtgatggc ggggccgtct gt
//
LOCUS       DAZFKY010000349         2372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFKY010000349 DAZFKY010000000
VERSION     DAZFKY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..414)
                     /locus_tag="ACHKCL_004885"
     CDS             complement(<1..414)
                     /locus_tag="ACHKCL_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5765666.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAE"
     gene            complement(411..701)
                     /locus_tag="ACHKCL_004886"
     CDS             complement(411..701)
                     /locus_tag="ACHKCL_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5765667.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(701..1300)
                     /locus_tag="ACHKCL_004887"
     CDS             complement(701..1300)
                     /locus_tag="ACHKCL_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO5765668.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
     gene            1800..>2372
                     /locus_tag="ACHKCL_004888"
     CDS             1800..>2372
                     /locus_tag="ACHKCL_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5765669.1"
                     /translation="MTENTTPTADFSPSKRFFVSMLTRDIDLNDAILDLLDNCVDGAL
                     RTIKDTKKTSKPYEGFYAKLTINKDVFIIEDNCGGIPKSFREYAFKMGRPHQKEEENE
                     GTVGVYGIGMKRAIFKMGRDCSIQSNNPDGAFTVDITPDWIDGDGWKIPMHESDYDNK
                     NPTGTTIEIKKLHSNVAQKFNESTYLTDLFL"
BASE COUNT          678 a          570 c          483 g          641 t
ORIGIN      
        1 ttcagccatt ttgcgcttag tcatcccccg ccccacataa cgctgactca gaacattgag
       61 cagcccggga taacctgcca gtacttcacc aataaccctg tcgattatta acgcctctga
      121 atcggtacaa tgcaccagcc agcttttttg cttgccgttg atcatatccc gcaaaaaagc
      181 ctcaagttta ggtttgtcca gacctgcttt tttcatcctc cggagcgcct cgttaattgc
      241 cgtttttgtc agctttttag aggtcagcaa ctggttgaac atatttcccg tcttaccgcc
      301 gccaatatac gaccagcgcc cccacatacg tagtttcccc tgaatccaga cactttccag
      361 cgtggtgaga cgaaggtgct ctccgctttt tcctgtgttc gttgggtaaa tcataaataa
      421 ccgtcctttc tccagatctc ttgtgtgcga aaaacacctt ctgcatgcat caggcgcaat
      481 tcttctttgg tgtaatcgct ggtttttact ctcccgtcga ttaaatcgtg gcatgagtta
      541 caggcaatcg ctgcctgcat atcgtgtggc tttatcgctg ttccgcacgt tcccgccagc
      601 ctgtaatgcg ccagcacaga ggtttcggga ttgtgattgc agtagccagg gattctgatc
      661 tggcatatct ggcctttagc cgctttacgt aaattcacca ttacgcaaac tccagtagtt
      721 gtgcggccac attttcaact tcctcctgag aggagaattt acggaacaga atccagttcc
      781 acagcacatt cagtacagat ttataaacct gctgaaactc gacttcgtcc atattcgcaa
      841 aagcgatgga ttttgcccga cgcccacggc taccgtccgg ataaatatgc tcggtgtaaa
      901 atccggcctg aatggttacc cactcgcgga aagcctcaaa cgactttagc aacgccgtat
      961 cccgggttct acgagtcgca acggtgttaa ggtattgctc tgcggcatca ctcagggctg
     1021 gcgtgtgttc ccgaccaact gattcgcaca ggtaatcaac gaaaccagac agcagttctc
     1081 gttcgcgagg cgtgatcgcc ccaccgaccg gagtccagta atcgaatccc agttgcagga
     1141 gtttgaaaaa acgcttgtgg aatgcgtagt tacgcacacg cttaaaatct gcgtgtatcc
     1201 actcacctat tttgatttga tgcagaaaat cgcaactctc cggcgtcgcc gggagaagta
     1261 aaccagaaga agtttgtttg accagttgta tatgcgccat ttctcaatct ctcgatggcg
     1321 cagtgcagca gatgccagtt gttcaagcta gcacgcaaat tgtaaaccag aatgccagga
     1381 aaaaacaaaa cccgccgaag ctggttgtgt gcggttgcgt tgaagtctcc tagccaaaga
     1441 gtagcggtga ccactccccg ccgataatct tagttgttta atttcgtaag ctaactaggc
     1501 taaaagctct ctgttgtctg cctgttgtac aattaacaaa atgaacaacg aactagatat
     1561 accaacgcca gaatggtgca acaaatccca aacaacacag caatgcaaca ataacatatg
     1621 ttattttcct ctcacgggat tcagtcctga attctcataa agctaatctt tttttaaaaa
     1681 catactgtta gatcacatgc actttctttc ttaacatcgt tgacttcgca tgcgaagcgt
     1741 ttaaaatcaa caatttgcac ttaaggtatt tattttccca aacacataga gagtaaatta
     1801 tgactgagaa cacgactcca acagcagact ttagtcctag taaaagattt tttgtaagta
     1861 tgctaactcg agatatcgac ttaaacgatg ccatacttga tctgcttgat aactgcgtcg
     1921 atggggcttt acgtacaata aaagacacaa aaaaaacttc aaaaccatat gaaggatttt
     1981 atgcgaaact tacgattaat aaagatgtct ttattataga agacaattgc ggaggtatcc
     2041 ctaaaagctt ccgagaatat gcttttaaaa tgggacgacc ccatcaaaaa gaagaagaaa
     2101 atgaaggcac cgttggcgtc tatggtattg gtatgaaaag agccattttt aaaatgggta
     2161 gagattgctc aatacaatcg aataatcccg atggcgcatt cactgtagat ataacacctg
     2221 attggatcga tggtgatggt tggaaaatac cgatgcacga gagtgactat gataacaaaa
     2281 atccaacagg cacaactata gagataaaaa aactccattc taatgtggca caaaaattta
     2341 atgagagcac atatcttact gatctctttt ta
//
LOCUS       DAZFKY010000350         2295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFKY010000350 DAZFKY010000000
VERSION     DAZFKY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..275)
                     /locus_tag="ACHKCL_004889"
     CDS             complement(<1..275)
                     /locus_tag="ACHKCL_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5765670.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHVSTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQITRYSDTRWHEDSV"
     gene            complement(346..>2295)
                     /locus_tag="ACHKCL_004890"
     CDS             complement(346..>2295)
                     /locus_tag="ACHKCL_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765671.1"
                     /translation="GAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWG
                     LSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTP
                     PAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTET
                     TYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPH
                     LAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSV
                     NTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTS
                     ITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGI
                     GAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTI
                     TSGGNPPAFSLTPDGRLTAKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGD
                     FVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSG
                     NNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKG
                     NQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          484 a          665 c          567 g          579 t
ORIGIN      
        1 acggaatctt catgccagcg ggtatcgctg taacgggtaa tctggcgatt cctgtctcct
       61 gcatagctga atgacgtcac cagccccagc gtgtccgtga attcataacg gtatttcacg
      121 ttaatcccgt taagattatc gctaccggga gcgttcgtcg agacatgaag ataccccgcg
      181 ctcagcgtgg cctgctgctc agacgcccat gcaggcgcac cggatacggt cagacaaatg
      241 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      301 aaaaaggcgt cattcctgac gccctttatt ggggttataa atatttcaac gaatactgat
      361 gccggaagca gcttttttgg tcacaatcac cgtacagtcg gtgatattgc ctgccccctg
      421 attgcctttc tggaaaatct taaactccag agtgacgcta ccaccaccac taggcatatc
      481 aataactgca ctgtaactac cgggaatggc ccctttagtt tctctggatg cgattaatac
      541 gccgtttttg cgaacttcaa aaccataccc cgtgtatcgc gtgcctcctg ggttatttcc
      601 gctccccgga tcgtcatacg ctataccgtt aaaaataatg ggcggaataa taatctggcg
      661 gtcaaagtta tgatcatcgc tgatggtgac tgtaaccgta ccgtttggtg tttccgtgtt
      721 accccacgta ccgacttttt tcgggaaggc ttttgataca gctttaacga aatcccctct
      781 gacctgggtc gcctccagca tgcccttaat cgtacagttc tggttaatcg tgacattgtt
      841 gagcgctcct gagttcgcat tcacactgcc actgatatcc gcatttttcg ccgtcagtcg
      901 cccgtctggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt
      961 cagatatttc aggaacactt cattcatgaa tatctgatcg ccctgaccaa caaacatcgg
     1021 ctttgtgttg ccattcgcag gattaatcat cgcaatcctg tctgctgcca gcagcacctg
     1081 actctgcatg ccgtcggggg tgttctcaat accggcaccg atacccgcaa tataaaggcg
     1141 tccgtcctgc atctgctgca gcttcactgc ccacatgctg ttcaggttat tatttgtatc
     1201 aacctgaacc ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac
     1261 ggtctgtgtt atttcattgc tgacatccgt tatggacgtc ctgatttcag tcaggtcagg
     1321 cgcaagctga ccgttatcaa tctgcgtcca cagctcctga gccagatggg ttttccctat
     1381 ctcgcctttg aaaaaatcca gatagcctga tgcatcatca ctcggctgac cgacagcctc
     1441 tacaaatgcc gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc
     1501 cggtttgata ttgatactgg cagctatcca gtacagcgcc gtgccaagat agcgggctgt
     1561 ggtttcaacc tgcctgatat cagcaatccg cttttccgag aaccagaact caaactgtac
     1621 cgtcggatca taaaccgcaa gatgcggcgt ggcggttatc tgaaaatagc ccggtgtcag
     1681 ctcaatccga gacggcgctg ccggtgcggc aatccggaac gataccgatg ccggatcgcc
     1741 ctgctgtccc cacgcattta ccgcccggac tgtcaacctg tagttccccg gcgccagttg
     1801 cgtgaagcgg tatgtggttt ctgtcgtcct ggccgtgctg accagccgct cactgccgtc
     1861 atccgctgcc acggtcaggc gaagcaggaa gctcacgccc ttcaccacct tcggcgtgtc
     1921 ccagcgcgcc agcacctgat attccccgct gtctgcggtg acttcggcgg tcaggtgctg
     1981 caccgctggc ggcgtgacac cattcaccgt gccgctctgg tcgccgtcaa agtgcgcccc
     2041 gttatccacg atggcttctt tttccggcac gtgctgcacc gccgtgatgg caaaggtgcc
     2101 gtccgtgttt tcccggatgg agacacagcg gaacaggcga cgacgcagtg acggcaggga
     2161 gagtccccat acaccgtatg tctccacacc atcaggcagg gtgctgacct gtatccggtc
     2221 cggcgcgggg tgtgcagtga tggccacgct caccggctta ccgctgccgt taatcaggtt
     2281 caccgtggcg gcacc
//
LOCUS       DAZFKY010000351         2258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFKY010000351 DAZFKY010000000
VERSION     DAZFKY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            87..269
                     /locus_tag="ACHKCL_004891"
     CDS             87..269
                     /locus_tag="ACHKCL_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5765672.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            348..848
                     /locus_tag="ACHKCL_004892"
     CDS             348..848
                     /locus_tag="ACHKCL_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5765673.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            885..1391
                     /locus_tag="ACHKCL_004893"
     CDS             885..1391
                     /locus_tag="ACHKCL_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5765674.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            1410..>2258
                     /locus_tag="ACHKCL_004894"
     CDS             1410..>2258
                     /locus_tag="ACHKCL_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFO5765675.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAEL"
BASE COUNT          744 a          432 c          496 g          586 t
ORIGIN      
        1 tgacactttc ttatttgtta ctagagttaa ttgggtgctg acaaggcaca tgttatttac
       61 aatttgaaaa tcagaggaga aatgatatga ccgaacatcg tggcggtgca ggtaatttcg
      121 ctgaagatcg acaaaaagct tctgacgctg gtcgtaaagg cggccaacat agtggcggta
      181 attttaaaaa cgatccacaa agagcatcag aagcagggaa aaaaggtggt cagaatagcc
      241 atggtggcgg ccgtaaatct gactcttaat ttatcacccc ctacattcgt gaataactga
      301 agctaactgc gagcctacgg gctcgcagtg aaaactctgg agtgattatg caaattaaat
      361 ctgttgaaga tatcttcatc cacttattat cggatacata cagcgctgag aaacaactaa
      421 caaaggcact gtctaaactt agtcgctctg catatagtga taaattaacc gctgcttttc
      481 agtcacatct tgatgagaca catggtcaaa ttgaacgcat cgatcaggtt gttgattctg
      541 aagatggtct taaattgaaa agaattaagt gtgccgcaat ggaaggactt atcgaagagg
      601 cgaatgaggt aattgaaagc actgataaaa atgaagtacg tgatgctgca ttgattgctg
      661 cagcacaaaa agtagagcac tatgaaattg ccagttatgg cactttggta acactcgctg
      721 agcaattagg atataaaaaa gcggctaaac tcctgaaaga aacactggaa gaggaaaaag
      781 ctactgatgt caaattaaca gacctggcat ttaataacgt taataaaaaa gctcaggata
      841 actcctgaac tatagacttc aatttctttt aggataaaca gattatgaat agaattgaac
      901 attaccatga ctggcttcgt gatgcacatg cgatggaaaa acaagctgaa aaaatgcttg
      961 agtctatggc cagccggata gaaaactatc ctgagttacg ttccagaatt gagcagcaca
     1021 ttagcgaaac aaagaaccag ctttcacagc ttgaaagcat acttgatcgt aataatattt
     1081 cgcgctctgt cattaaagac tctatgagta aaatggccgc gtttggacaa tccattggtg
     1141 gaatttttcc atctgatgaa attgtcaaag gttcgatcag tggctatgtg ttcgaacaat
     1201 ttgaaattgc gtgttacaca tctcttctcg cagcagccaa aaatgcgggt gatacagctt
     1261 cagtacccat tattgaagca attcttaatg aagaaaaaca aatggctgaa tggcttctta
     1321 accatattcc tgacaccact gagcaattca tggtcagatc tgaaattgat ggagttgaag
     1381 ccaaaaaata attcttttga ggaggcatta tgtttagaca cgtgaaaaaa cttcaatata
     1441 cagtacgtgt aagtgagcca aacccaggct tggctaatct actcttagaa caattcggtg
     1501 ggccacaggg tgagcttgca gcagcatgcc gatacttcac tcaaggctta agtgatgaag
     1561 atgcaggccg aagggatatg ctgatggaca tagcaacaga agaactaagt catctcgaaa
     1621 ttattggttc tcttgttggt atgctcaaca aaggtgcaaa aggagcactt gctgaaggaa
     1681 ctgagaatga agctgagctt taccgttcct taactcaaaa tggcaatgat agccatataa
     1741 cttctctcct ctacggagga ggcccagcat taactaattc agctggagta ccctggactg
     1801 cagcttatgt tgatactatt ggcgaagtca cggccgatct tcgttcaaat attgcagctg
     1861 aagctcgggc aaagatcatt tacgaacgtc tcattaatgt tactgatgat cctggagtaa
     1921 aagacactct ggcattttta atgacccggg aagcagcaca catgctttca tttgaaaaag
     1981 cattacattc aattcgtaac acctttccac caggaaaatt accaccactt gagaaataca
     2041 aaaacgtata ttacaacatg tcagaaggtg aagacgtcag aggtagttgg aacagtgatg
     2101 aaaactttga ctatgtttcc gatccagttt cagcggttga cggtggggat gggaaggcct
     2161 cgataaatct ttcgaccaag caagaagcta tgataaaagc tatggcaaca aggttgaaat
     2221 cccatgaaga catcaatcct gtcactggcg ctgagctt
//
LOCUS       DAZFKY010000352         2240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFKY010000352 DAZFKY010000000
VERSION     DAZFKY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1668
                     /locus_tag="ACHKCL_004895"
     CDS             <1..1668
                     /locus_tag="ACHKCL_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765676.1"
                     /translation="ARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVV
                     KGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPAT
                     TTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSAR
                     YLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKI
                     GKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQ
                     KDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDG
                     NTTPLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            1736..>2240
                     /locus_tag="ACHKCL_004896"
     CDS             1736..>2240
                     /locus_tag="ACHKCL_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5765677.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNP"
BASE COUNT          533 a          556 c          669 g          482 t
ORIGIN      
        1 gcccgctttg agccgcagtc aggcaccctg aacagcgtca tccctccggc agtgcagcac
       61 ctgacggtgg aggtgagcgc agctgacggc cagtatctgg cgcaggctaa atgggacacg
      121 ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagcggaag cggagaaggc
      181 agccgtctgg tgaccaccgc catcactgcg gatacagagc atcgttccag tggtctgccg
      241 ccgggggaat acaccctgac ggtcagggcg attaacagtt atggccagca gggggaaccg
      301 gccaccacca cgttcaggat taatgcacct gcggtacccg ccacgattga gctgacaccg
      361 ggctattttc agataacagc ggtcccgcgt cttgcggtgt atgacccgac ggtacagttt
      421 gagttctggt tttcggagac aaaaatcgca gatacatctc aggtggaaac ctctgcccgt
      481 tatctgggga ccggcagtca gtggagtgta tccggcccgc acattaagcc cgggaaggat
      541 ttctggtttt acgtgcgcag cgtcaacctg gtggggaaat ctgcttttgt ggaagccagt
      601 ggccgggcca gcaatgatgc agaagggtat ctggggctgt ttcgggaaaa aataggaaaa
      661 ctgcatctgg ctcaggggct gtgggagctg atagacaaca gccagcttgc ggatgagatg
      721 gcggagatga agacctccat caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa
      781 acgctggagg accagagcgc catcatacag cagatacagc gcgtgcagaa ggacacaaat
      841 gatgaccttg ctgcacttta catgctgaag gtacagaaaa caaaaaatgg cataccctat
      901 gttgccggta ttggagcggg gattgaggat actgatggcc agcccctgag caacatactg
      961 ctgctggctg accgtattgc gatgattaac ccggaggacg gcaacaccac gccgttattt
     1021 gtggcgcagg ggaatcagtt gttcatgaac gatgtgttcc tgaaacgact gtttgcggtg
     1081 agtatcacgt catccgccaa tcccccgacg ttttccctga cgccggaggg caggctgacc
     1141 gcaagaaatg ctgatatcag cggtaacgtg aatgcgaatt ccgggacgct caacaacgtc
     1201 acgattaacg agaactgtcg ggttctggga aaactgtccg cgaaccagat tgaaggcgat
     1261 ctcgttaaaa cagtgggcaa agctttcccc cgggattccc gtgcaccgga gcggtggcca
     1321 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgaccggca gattgttatt
     1381 ccggcggtgg cattcagcgg cgctaaacat gagagagagc atactgatat ttactcctca
     1441 tgccgtctga tagtgcggaa aaacggtgct gaaatttata accgtaccgc gctggataat
     1501 acgctgattt acagtggcgt tattgatatg cctgccggtc acggtcacat gacgctggag
     1561 ttttcggtgt cagcatggct ggtgaataac tggtatccca cagcaagtat cagcgatttg
     1621 ctggttgtgg tgatgaagaa agccaccgca ggcatcagta tcagctgaat tttataaccc
     1681 atatacgggc gccagaaatg gcgccttttt tattgcagaa aagcgagagg taattatgcg
     1741 taaactttat gccgccattt tgtccgcagc catttgtctg gccgtatccg gtgcgcctgc
     1801 atgggcgtct gaacatcagt caacgctgag cgcggggtat cttcatgtct cgacgaacgt
     1861 tcctggcagc gatgaactga acgggattaa cgtgaaatac cgttatgagt ttacggacac
     1921 actggggatg gtgacgtcgt tcagctatgc aggagacaag aatcgccagc tgacccgtta
     1981 cagcgatacc cgctggcatg aagattccgt tcgtaaccgc tggttcagcg taatggcggg
     2041 gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag
     2101 ccgtgtgtcg actttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
     2161 tgatgtgctg accggaagtg atgacggtcg ccacagcaac acgtctctgg cgtggggagc
     2221 tggcgtgcag tttaacccga
//
LOCUS       DAZFKY010000353         2199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFKY010000353 DAZFKY010000000
VERSION     DAZFKY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..423
                     /locus_tag="ACHKCL_004897"
     CDS             <1..423
                     /locus_tag="ACHKCL_004897"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5765678.1"
                     /translation="AASDKNALCAHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVE
                     KAAEQCIQQRQTVVMLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGLGVRRAA"
     gene            420..596
                     /locus_tag="ACHKCL_004898"
     CDS             420..596
                     /locus_tag="ACHKCL_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5765679.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            599..1000
                     /locus_tag="ACHKCL_004899"
     CDS             599..1000
                     /locus_tag="ACHKCL_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765680.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            960..1169
                     /locus_tag="ACHKCL_004900"
     CDS             960..1169
                     /locus_tag="ACHKCL_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5765681.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDG"
     gene            1162..1767
                     /locus_tag="ACHKCL_004901"
     CDS             1162..1767
                     /locus_tag="ACHKCL_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5765682.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALKLQSKQRK
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLMSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTIEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            1764..1958
                     /locus_tag="ACHKCL_004902"
     CDS             1764..1958
                     /locus_tag="ACHKCL_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5765683.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            1951..>2199
                     /locus_tag="ACHKCL_004903"
     CDS             1951..>2199
                     /locus_tag="ACHKCL_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5765684.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGGTFMA"
BASE COUNT          693 a          493 c          575 g          438 t
ORIGIN      
        1 gctgcgagcg acaaaaacgc tctgtgtgct cactggctaa ctgaggctga cgacgcgcta
       61 aattctgagt ggataagcca cggtgcaatc tggaataacc caccgtacag caatatcagg
      121 ccgtgggtgg aaaaagccgc tgagcagtgc atacaacagc gacagacggt agtgatgctt
      181 gtgccagagg atatgtctgt cggatggttc agcaaggctc tggagagtgt tgacgaagtt
      241 cgcatcatca ctgatggacg gattaatttt atcgaaccat cgacagggct ggagaagaag
      301 ggaaacagca aaggctcaat gttgctgatt tggcgaccgt tcatcagtcc tcgacggatg
      361 tttactaccg tatccaaagc ggcattgatg gcgatcgggc tgggcgtcag gagggctgca
      421 tgaggcgaca gcgacgaagt atcaccgaca tcatctgcga aaactgcaaa taccttccaa
      481 cgaaacgctc cagaaataaa cgcaagccaa tcccaaaaga atctgacgta aaaaccttca
      541 attacacggc tcacctgtgg gatatccggt ggctaagaca tcgtgcgagg aaatgacaat
      601 gcttttaatt caacctggat ttggacttag catcaaaaaa ggccacatgt ttggcgagaa
      661 agagtcacaa cgaaaaatgg tgtctatccg gttgccattt atcagtattt attggctaaa
      721 cagggaggca acaaattatt ggtatacatg cgccagagca gcatttaacg accctgactg
      781 gtttgtgaaa aaccaccacg cagttcgtca ggcaaagaga aaggccaata tgacatacat
      841 gaaggcgtat caaaaagcat ggaaagaaca ccgcgatcga taccaacaag acatggaaaa
      901 gcttgaatca gaaaacatgg aattaagacg aaagctcggt gaagcaaaac gagacattga
      961 tgcttacaag cgacttttta atggtgaaag ccatgcttag cccatcccaa tcccttcaat
     1021 accagaaaga aagcgtcgag cgggctttaa cgtgcgctaa ctgcggtcag aagctgcatg
     1081 tgctggaagt tcacgtgtgt gagcactgct gtgcagaact gatgagcgat ccgaatagct
     1141 caatgtacga ggaagaagac gatggctaaa ccagcgcgaa ggaaatgcaa aatatgcaag
     1201 gaatggtttc acccggcatt ctcaaatcag tggtggtgct gcccggaaca cggaactcag
     1261 ttagcactca aactacaaag taaacagcga aaaaaagcgg aaaaagcagc agagaagaaa
     1321 cgacgacgag aggagcagaa acagaaagat aaactgaaga ttcgaaaact cgccttaaag
     1381 ccccgcagtt actggattaa acaagcccaa caagccgtaa acgccttcat cagagaaaga
     1441 gaccgcgact taccatgtat ctcgtgcgga acgctcatgt ctgctcagtg ggatgccgga
     1501 cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa tattcacaag
     1561 caatgcgtgg tgtgcaacca gcataaaagc ggaaatctcg ttccctatcg cgtcgaactg
     1621 attaaccgaa tcgggcagga agcagtagac gaaatcgaat caaaccataa ccgccatcgc
     1681 tggactatcg aagagtgcag ggccatcaag gcgaagtatc agcagaaact taaagacctg
     1741 cgaaacagca gaagtgaggc cgcatgacgt tcacagtaaa aaccattcct gacatgctcg
     1801 ttgaggcata tgaaaatcag accgaggtag ccagaatact gaactgtagt cgcaacacgg
     1861 tcagaaaata cactggcgat aaagaaggaa aaagacacgc tattgtcaac ggtgttctta
     1921 tggttcatcg cggatggggt aaagatactg atgcgtgata tccggcaggt tcttgagtgc
     1981 tggggggcat gggcggcaaa taaccatgag gatgtgacct ggtcacccat tgccgccgga
     2041 tttaagggac tgatccccga aaaagtaaaa tcacgcccgc agtgctgtga cgatgatgcg
     2101 atggtgatat gcgggtgtat agcccgcctt taccggaaca atcgcgatct gcatgacttg
     2161 ctggttgatt attacgtgct gggggggacg ttcatggcg
//
LOCUS       DAZFKY010000354         2155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFKY010000354 DAZFKY010000000
VERSION     DAZFKY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            77..199
                     /locus_tag="ACHKCL_004904"
     CDS             77..199
                     /locus_tag="ACHKCL_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5765685.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            214..903
                     /locus_tag="ACHKCL_004905"
     CDS             214..903
                     /locus_tag="ACHKCL_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5765686.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            1087..1830
                     /locus_tag="ACHKCL_004906"
     CDS             1087..1830
                     /locus_tag="ACHKCL_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765687.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            1916..2083
                     /locus_tag="ACHKCL_004907"
     CDS             1916..2083
                     /locus_tag="ACHKCL_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5765688.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PVIKRNSLHNA"
BASE COUNT          570 a          358 c          528 g          699 t
ORIGIN      
        1 gttacaaaaa aagggggggc aattacaggt agttatggat gatgagtgaa acagatattg
       61 gagaaccggg gaatgaatga tgtctgagtc ttatatatca gaactccttc gctgtcgctg
      121 ggggctcctg tgcttatgtc gtttccccga ttcggttttg aacgattacc gaatgttgaa
      181 gaattatgcc aaaatataga aaggatttac tgcatgaata cccaatattt acagtatgtt
      241 cgtgagcaac ttatggcagc tactgctgac ttgaacggag caacgaaagg ccagctcgaa
      301 gcctggcagg agcatgcaca atttgatact ggtacataca aacgaaagaa gccgcgcatt
      361 ctggatgtgg taactggcaa gatgattacg ctggataata cgccgacttc cggtaagcag
      421 tcgtacgcaa aaggttcatc cattgctttg gtcagcccgg ttgaattctc aacctcttca
      481 tggcgccgcg cggttttgtc tctcgatgaa catcagaaag catggttgct ttggtgttac
      541 agcgaaagcg ttcgatgggg gcatcaggtc accataacgc aatgggcatg gagcgagttt
      601 aaagatttgt taagtaacag aaaaattgca ggtaagacac tggatcgcct gaagacgtta
      661 atctggctgg ctgcacagga tgtgaagagc gaacttgcag ggcgtgaggc ctatgaatac
      721 cagacactgg catcattggt gggagtgaca acaaaaaact ggtccgagac atttactgaa
      781 cgctgggttg caatgaagca catttttcta cagcttgata gtgatgcttt attgcttgtg
      841 acgagaacac gttcaaaaca aaaggcagca tttttacagc aaaatattgc aaaactggat
      901 taaaagccat atacttcatg caaatttggt atgttgtaaa aaatgtataa acccgctgcc
      961 gagtgggttt ttttatgccc tgagttgtac ttgtacggta aacatgctgg ctgctatgta
     1021 atagagtttt tttagcctgt aacctcttga cggcattgaa ttgcttttgt tatgagttgt
     1081 aagccaatgt tatcatcttg tattggggtg gttatgaagg atggtgcgct gctcaggagt
     1141 tcttcacttt ttattgccta catgggatgc cttggatggg ggagtgctta tttctatgga
     1201 tggggtactt ctttttacta cggcttccca tggtggattg taggtgcagg tgttgatgat
     1261 gttgccagaa gtttattttt tgcagttatc gtcattgcta tatttcttat cggttggggt
     1321 attggtgttg tattcttttt cgcagtgaaa agaaaacatt ctatgcaaga gctaaatgta
     1381 tttcgccttt attttgctgt ggaattattg tttgtgccgg caattattga gttttctata
     1441 ttgagacaga agattcaggt acctcttttg ctactgtcag cagcgattgc gctggcggtt
     1501 acaatttcga taagatctta tgggcgattt ttatcggtat catgcttcta tgataagcca
     1561 tttataaaaa aacatttttt tgagattgtg atgattgctt ttgtggcata tttctggctt
     1621 ttttcatttc tgacaggata ttacaaaccg cagtttaaga aagaatatga aatgattaat
     1681 tataatgatg gttggtatta tgttcttgct cgttatgata attgtctggt tttgtctact
     1741 tctttcaatg caggtagtaa aaggtttgtg atttatcaat cagcacaaga taagaatctt
     1801 caggttgata ttgtaaggac cagaatttaa ttggctgcat aaataatatt ttaagttgca
     1861 agttggctat tcgtaggaat agaaccttag gcatgctgaa tgcgtttcct gaacattgtt
     1921 ttataaactg tgtctgcttg ctgttgtgat cctgctttta gtgatggtga tgatggattt
     1981 caccagcagg ataatgttgg tactgactga tggcgctctg gtctgcggca ttgtggtatt
     2041 gctgtggccg gtgataaaaa gaaacagcct gcataatgct tgattttttt gtttactgtt
     2101 tattaaaaat actactgcat ggcgaatccc cctgtgcgga ggggcaatca gcaac
//
LOCUS       DAZFKY010000355         2130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFKY010000355 DAZFKY010000000
VERSION     DAZFKY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..717
                     /locus_tag="ACHKCL_004908"
     CDS             172..717
                     /locus_tag="ACHKCL_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5765689.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            692..>2130
                     /locus_tag="ACHKCL_004909"
     CDS             692..>2130
                     /locus_tag="ACHKCL_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5765690.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
BASE COUNT          517 a          511 c          615 g          487 t
ORIGIN      
        1 ttttcactat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt cgccgtaacc
       61 taatgttttt atttaaaaca ccccctgaaa agaaaggaaa cgacaggtgc tgaaaacggg
      121 ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac aatggaagtc
      181 aacaaaaagc agctggctga cattttcggc gcgagtatcc gtaccattca gaactggcag
      241 gagcagggaa tgcccgttct gcggggtggt ggcaagggta atgaggtact ttatgactct
      301 gccgccgcca taaaatggta tgccgaaagg gatgctgaaa ttgagaacga aaagctgcgc
      361 cgggaagttg aagaactgag gcaggacagc gagacagatc tccagccagg gactattgag
      421 tacgaacgcc atcgacttac gcgtgcgcag gccgacgcac aggaactgaa gaatgccaga
      481 gactcagctg aagtggtgga aaccgcattc tgtactttcg tgctgtcgcg gatcgcaggt
      541 gaaattgcca gtattctcga cgggatcccc ctgtcggtgc agcggcgttt tccggaactg
      601 gaaaaccgac atgttgattt cctgaaacgg gatatcatca aagccatgaa caaagcagcc
      661 gcgctggatg aactgatacc ggggttgctg agtgaatata tcgaacagtc aggttaacag
      721 gctgcggcat tttgtccgcg ccgggcttcg ctcactgttc aggccggagc cacagaccgc
      781 cgttgaatgg gcggatgcca gttactatct cccgaaagaa tccgcatacc aggaagggcg
      841 ctgggaaaca ctgccctttc agcgggccat catgaatgcg atgggcagtg actacatccg
      901 cgaggtgaat gtggtgaagt ctgcccgtgt tggttattcc aaaatgctgc tgggtgttta
      961 tgcctacttc atagagcata agcagcgcaa cacccttatc tggttgccga cggatggtga
     1021 tgccgagaac tttatgaaaa cccacgttga gccgactatt cgtgatattc cgtcgctgct
     1081 ggcgctggcc ccgtggtatg gcaaaaagca ccgggataac acgctcacca tgaagcgttt
     1141 caccaatggg cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa
     1201 gtcagtggat gtggcgggtt atgatgaact tgctgccttt gatgaggata ttgaacagga
     1261 aggctctccg acgttcctgg gcgataagcg tattgaaggc tcggtctggc caaagtccat
     1321 ccgtggctcc acgcccaaag tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc
     1381 cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc agtaccttaa
     1441 atttggcgac aaagagacgc cgtttggcct caaatggacg ccggatgatc cctccagcgt
     1501 gttttatctc tgcgagcata atgcctgcgt catccgtcag caggagctgg actttactga
     1561 tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc tctggttttc
     1621 gtcatccggt gaagagattg aaccgcctga cagtgtgacc tttcacatct ggacggcgta
     1681 cagcccgttc accacctggg ttcagattgt caaagactgg atgaagacga aaggggatac
     1741 gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acatgggaag cgaagatcgg
     1801 cgaacgtccg gatgctgaag tgatggcaga gcggaaagag cattattcag cgtccgttcc
     1861 tgaccgtgtg gcttacctga ccgccggtat cgactcccag ctggatcgct acgaaatgcg
     1921 cgtatgggga tgggggccgg gtgaggaaag ctggctgatt gatcggcaga ttattatggg
     1981 ccgccacgac gatgaacaga cgctgctgcg tgtggatgag gccatcaata aaacctatac
     2041 ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg gcgggattga
     2101 cccgactatt gtgtatgaac gctcgaaaaa
//
LOCUS       DAZFKY010000356         2128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFKY010000356 DAZFKY010000000
VERSION     DAZFKY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(31..420)
                     /locus_tag="ACHKCL_004910"
     CDS             complement(31..420)
                     /locus_tag="ACHKCL_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765691.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTPIVFILKLIGATH"
     gene            complement(548..1261)
                     /locus_tag="ACHKCL_004911"
     CDS             complement(548..1261)
                     /locus_tag="ACHKCL_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5765692.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1362..1562
                     /locus_tag="ACHKCL_004912"
     CDS             1362..1562
                     /locus_tag="ACHKCL_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5765693.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1681..1974
                     /locus_tag="ACHKCL_004913"
     CDS             1681..1974
                     /locus_tag="ACHKCL_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO5765694.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            2007..>2128
                     /locus_tag="ACHKCL_004914"
     CDS             2007..>2128
                     /locus_tag="ACHKCL_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5765695.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYS"
BASE COUNT          605 a          469 c          470 g          584 t
ORIGIN      
        1 gaggtgatcg agagatcaga attgcgttgt ttagtgagtt gcacctatta attttaaaat
       61 aaatacaatt ggtgtttttc gagtttttct taactcttca tgcagctccg ttctcagaat
      121 atatgatttt tcgagtttga aattcttgcg accaggcgtc ttattgccag gctcaggaag
      181 tggaaggcgt gtttgaatgt cgagtggtgg acagtcatag aagtaaattc tataaagctc
      241 aagcggttcc ctacgttctt gtgattgacg ttttccatta agatgggaaa gaaccattga
      301 ccatattact ttcattatgc attgcgcagt cagttcatgc tcggcgaagt gtttacgatg
      361 cgtagcatga acacgctgca tgaaaaaacc cgcatcaatt aaaattgctg ctttcttcat
      421 aagagtccca aaaaaatagc ccagagccgt tatgcagata ttaacaattg tctgcgaacg
      481 gggctgggct tggtttaatt aatctatgcc aagttgactg tgccgtcaac ataaaactta
      541 cttcctatca cccaaacgtc tcttcaggcc actgactagc gataactttc cccacaacgg
      601 aacaactctc attgcatggg atcattgggt attgtgggtt tagtggctgt agaaacacct
      661 gaccgctatc cctgatcagt ttcttgaagg taaactcatc accaccaagt ctggctatac
      721 agaaatcgcc tggctcaaca gcctgctcag ggtcaaccag aattaacatc ccgtcaggaa
      781 agctgggctt ggatcctgtt ggtgcggtca tggaattacc ttcaacctca agccagaatg
      841 cagagccact ggcttttttg gttgtgctta cccatttctc cgcatcgcct ttggtaaagg
      901 ttctaagctc aggcgagaac atcccggcct gaacatgaga aaaaacaggg tactcatact
      961 cacttctaag tgacggctgc atactaaccg cttcatacat ctcgtagatt tctctggcga
     1021 ttgaagggct aaattcttca acgctaacgt tgagaatttt tgcaagcaat gcggcgttat
     1081 aagcatttaa tgcattgatg ccattaaata aagcaccaac gcctgactgc cccatcccca
     1141 tcttgtctgc gacagattcc tgggataagc caagttcatt tttctttttt tcataaatag
     1201 ctttaaggcg acgtgcgtcc tcaagctgct cttgtgttaa tggtttcttt tttgcgctca
     1261 tacgttaaat ctatcaccgc aagggataaa tatctaacac cgcgcgtgtt gactacttta
     1321 cctctggcgg tgataatggt tgcatgtact aaggaggttg tatggaacaa cgcataaccc
     1381 tgaaagatta tgcaatgcgc tttgggcaaa ccaagacagc taaagatctc ggcgtatatc
     1441 aaagcgcgat caacaaggcc attcatgcag gccgaaagat ttttttaact ataaacgctg
     1501 atggaagcgt ttatgcggaa gaggtaaagc ccttcccgag taacaaaaaa acaacagcat
     1561 aaataacccc gctcttacac attccagccc tgaaaaaggg catccaatta aaccacacct
     1621 atggtgtatg catttatttg catacattca atcaattgtt atctaaggaa atacttacat
     1681 atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa
     1741 atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gagttgataa gtcgcagatc
     1801 agcaggtgga agagagactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg
     1861 ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat
     1921 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1981 tactggatct atcaacagga gtcattatga caaatacagc aaaaatactc aacttcggca
     2041 gaggtaactt tgccggacag gagcgtaatg tggcagatct cgatgatggt tacgccagac
     2101 tatcaaatat gctgcttgag gcttattc
//
LOCUS       DAZFKY010000357         2108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFKY010000357 DAZFKY010000000
VERSION     DAZFKY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1439)
                     /locus_tag="ACHKCL_004915"
     CDS             complement(<1..1439)
                     /locus_tag="ACHKCL_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5765696.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
     gene            complement(1414..1959)
                     /locus_tag="ACHKCL_004916"
     CDS             complement(1414..1959)
                     /locus_tag="ACHKCL_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5765697.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYECHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          487 a          602 c          503 g          516 t
ORIGIN      
        1 tttttcgagc gttcatacac aatggtcggg tcaatcccgc cagtatccca gcagatacgg
       61 gataccgaca tttctgcacc attccggcgg gtataggttt tattgatggc ctcatccaca
      121 cgcagcagcg tctgttcatc gtcgtggcgg cccataataa tctgccggtc aatcagccag
      181 ctttcctcac ccggccccca tccccatacg cgcatttcgt agcggtccag ctgggagtcg
      241 ataccggcgg tcaggtaagc cacacgatca ggaacggacg ctgaataatg ctctttccgc
      301 tctgccatca cttcagcatc cggacgttcg ccgattttcg cttcccacgt ctcaccgagc
      361 gtggtgttca cgaaggtttt acgttttccc gtatcccctt tcgtcttcat ccagtctttg
      421 acaatctgca cccaggtggt gaacgggctg tacgccgtcc agatgtgaaa ggtcacactg
      481 tcaggcggtt caatctcttc accggatgac gaaaaccaga gaatgccatc acgggtccag
      541 atcccggtct tttcgcagat ataacgggca tcagtaaagt ccagctcctg ctggcggatg
      601 acgcaggcat tatgctcgca gagataaaac acgctggagg ggtcatccgg cgtccatttg
      661 aggccaaacg gcgtctcttt gtcgccaaat ttaagatact gctcctcccc gcagtgcggg
      721 caggcaacat gaaaacgcat aaaatgcggg gattcactgg ctgcacgctc aatctggcag
      781 gtgcctctca ctttgggcgt ggagccacgg atggactttg gccagaccga gccttcaata
      841 cgcttgtcac ccaggaacgt cggagagcct tcctgttcaa tatcctcatc aaaggcagca
      901 agttcatcat aacccgccac atcaaccgac ttttcacggt agttttttgc cgctttaccg
      961 cccaggcacc agaagccacg accattggtg aaacgcttca tagtgagcgt gttatcccgg
     1021 tgctttttgc cataccacgg ggccagagac agcagcgacg gaatatcgcg gatggtaggc
     1081 tcgacgtggg ttttcataaa gttctcggca tcaccatccg tcggcaacca gataagggtg
     1141 ttacgctgct tatgctctat gaagtaggca taaacaccca gcagcatttt tgaataacca
     1201 acacgggcag acttcaccac attcacctcg cggatgtagt cgctgcccat cgcattcatg
     1261 atagcccgct gaaagggtag tgtttcccag cgcccttcct ggtatgcgga ttctttcggg
     1321 agatagtaac tggcatccgc ccattcaacg gcagtctgtg gctccggcct gaacagggct
     1381 cgcagcccgg cgcgtacatc acgccgcaga atatcaatct gactgttcga tatattcact
     1441 cagcaacccc ggtatcagtt catccagcgc ggctgctttg ttcatggctt tgataatatc
     1501 ccgtttcagg aaatcaacat gtcggttttc cagttccgga aaacgccgct gtactgagag
     1561 agggatcccg tcaagaatac tggcaatttc acctgcgatc cgtgacagca cgaaagtaca
     1621 gaatgcggtt tccaccactt ctgcggagtc tctggcattc ttcagctcct gggcgtcagc
     1681 ctgcgcacgc gtaagtcgat ggcattcgta ctcaatagtc ccaggctgga gatctgcctc
     1741 gctggcagcc ctgtaatcct caacctcttt acggagtttt tcattttcga tatcagcttc
     1801 cctctgcgca taccactgaa ttgccgtggc ggtatcaaat acagattcaa cgcccttacc
     1861 acctccggag acgcaaggga gcccctgaga ctgccagcgt tcaatcgttc gcggatccac
     1921 gttgaaaatt tcggcaagtt tctttttatt aaccttcatg aaacagtctc acaacaaata
     1981 cagggtccga catgaaagtg cccgaaaatg actttttcag gcgttttcat gtcggacctt
     2041 tacggattcg atattagaaa aaacaaatag ttatgttcga gaagtaccga catgattttc
     2101 cccggaaa
//
LOCUS       DAZFKY010000358         2090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFKY010000358 DAZFKY010000000
VERSION     DAZFKY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1229)
                     /locus_tag="ACHKCL_004917"
     CDS             complement(<1..1229)
                     /locus_tag="ACHKCL_004917"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5765698.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTAL"
     gene            complement(1210..>2090)
                     /locus_tag="ACHKCL_004918"
     CDS             complement(1210..>2090)
                     /locus_tag="ACHKCL_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765699.1"
                     /translation="DGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESE
                     LDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQT
                     AQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRK
                     FVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLK
                     EVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFES
                     GLRQSTEEEKSDSRAA"
BASE COUNT          387 a          681 c          573 g          449 t
ORIGIN      
        1 agcgcagtat cactgcgcgc ctgtgcactc tgtggtgctg cggccagaat gcggcgggcc
       61 gtttccacgg tcataccggg ggtttctgcc agcacgcggg cctgttcttc gcgtccgtga
      121 gcctcctcac agttgaggat ccccataatg cggctgtttt ctgccgcaac cgcagcggtg
      181 atctgcgcgt tcacgtccgg ctgcgccgcg ctggcgtttt cgccctccgt cgctggcacc
      241 acgccagtaa cgtcagcctg cgaagcagtg gctgaaacag ttgttgattg agtctctttg
      301 gtcattcgcc ctcctgagag acgggattta cgtgcatcca gtgcatcacg cataacggtg
      361 atcgcatcgg tgctgttgac aagttcatca gccagtccgg catcaatggc ctcctgaccg
      421 ctgtacactg cagcctcggt atccagcaca gcctgcacgg acaggccggt atatgccgac
      481 accttctgcg caaacatccg gcgggttgca tccatccggg actgcagtgt ttcccggaca
      541 tcacccggta gatggctgta ggggttgcca tccaccttat ggctgccgct gtaaatcagc
      601 gtgatttcca cgccctgttt ctccagggca gcaccgtaat tactgtgagc catcatgacg
      661 ccgatggagc ctgtccgggc ggtctgcgtg accagacgcc gggaggcggc gctggcaagc
      721 agctgacctg cactgcagtt catgtcgttg gccagcgccc ataccggttt tatgtctcgc
      781 acacgggcga tgatgtcagc acagtcaaat gctcccgcca ccatcccgcc cggtgtgtcc
      841 atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag acgggcgata
      901 atgccgttgt aaccggtcat tcccgaatac ggctgcagcg cccgcgtccg gctgaccagc
      961 gtaccggaca ccggcagcac ggcgatgccg ttcatgacct gataactccg ggcctgtcgt
     1021 ggtccgtcat catcaccgga taacgccagc gccgcgggtg cctctccggc agtcaggctg
     1081 tcgccggata ctgcatccgt caggcggctg atcccaagct ggcctgcaag cgcacaaaag
     1141 aaaacccgcg cataggcggg ttcaagcatc agcggctcat taaaagccat gctggcaata
     1201 tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt gtcgcagccc
     1261 ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccagctgcgc ggcgctccat
     1321 cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt tcgcgcactc
     1381 tttctcgtag gtgctcagtc cggcttctat cagcatcacc gcttcctgaa cttctttcag
     1441 accatcgatg gccatacgac cggagcctat ccagtcgcag ttcccccagg cactgcgggc
     1501 ttcctgaaag ctgaagcgcg cttttgaagg taacgtcacc acgcggcgaa cgatggcctc
     1561 ttccagccag cacagaaaca tctggctcgc ctgacgggat gcgacgaatt ttcgccgccc
     1621 cataaagtac gcccacgact cgttcgcact ggcccgtgcc gtggagtagc tcatctgggc
     1681 gtaattccgg gaaagctgct catacgagac acccagcccg gcagcgatat accgcagcag
     1741 tgactgctca aacacggagt agccgttatc cgtgtcctga gccgtctgca ggttcagtga
     1801 gtcccccggc atcaggtgcg gcacttttgc gcctcccaga cggaccggtg ctgcggcgta
     1861 atacgcggca atttcaccaa tccagccggt cagcctttcc cgctgctcct gactgttcgc
     1921 gcccagaata aaatccatcg ctgactgcgt atccagctca ctctcaatgg tggcggcata
     1981 catcgccttc acaatggcgc tctgcagctg cgtgttctgc agcgtgtcga gcatcttcat
     2041 ctgctccatc acgctgtaaa acacatttgc accgcgggtc tgcccgtcct
//
LOCUS       DAZFKY010000359         2060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFKY010000359 DAZFKY010000000
VERSION     DAZFKY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>2060
                     /locus_tag="ACHKCL_004919"
     CDS             <1..>2060
                     /locus_tag="ACHKCL_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO5765700.1"
                     /translation="DIKPISEIQQPLHSILSRQKEHVKNLLSGLLDEFSNKLRKQGLS
                     LKTNVLSVNNFKESKINSDTVEVTVTDLQGRLYRVDIDTRVIGLTFKEGINSLSEALE
                     HMNIDAIMSVIGLVQYARMIKMNDNISAIDHAGAVSDIKNIVDKFLGGILTLTNNRVY
                     NPGGVSGASLEGFISSGLEVCASRMGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDSS
                     LNHAKATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAVY
                     VNQINERHKLWLEAEKYLDNGSAKVLSINKATGIIDLSNNQVLGNIYLDMRENPPILH
                     GEKSYNSGKNIGSHPDMTDREIMESRAYNFACTKLSDAGEPDIFGWGDKEICNSMELS
                     ESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAYT
                     DDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLHMIERFKNYNFSISGGK
                     GGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDGRKQNFNFHHPSGFYS
                     GKVMSITQKGINAVVGTKLGYDKIRGNNLDNTFSLGSGGGVIYSGGGSNTYFVPATLQ
                     DNLHIYISEKSNGNHIILGDMHSRLSIECHFSNNEREFIKMGYYNGCDVILESDTIQK
                     IKSFA"
BASE COUNT          706 a          307 c          408 g          639 t
ORIGIN      
        1 gatattaaac ctataagtga aatccaacag ccccttcatt ccattctttc acggcagaaa
       61 gaacacgtaa agaatttact atctggattg cttgatgagt tttcaaataa gttaagaaag
      121 caaggtcttt cacttaaaac aaatgttctt tcagttaata acttcaaaga atcaaagata
      181 aatagtgata cagtggaggt tacagtaaca gatctgcaag gaagacttta tcgtgttgat
      241 atagatacaa gagttattgg cttaactttt aaagagggga taaatagttt atccgaagca
      301 ctcgaacaca tgaatatcga tgcgattatg tctgttattg gcttggttca atatgcacga
      361 atgataaaaa tgaatgacaa tattagtgca atagatcatg caggagcagt atcagatata
      421 aaaaatattg tagacaaatt tttaggcgga attttgactc ttactaataa tcgcgtatat
      481 aatcctggcg gtgtatctgg tgcctcgctt gaaggattta tatcttctgg gttggaggtg
      541 tgtgcttcaa gaatgggtgg caccgcaggg cgttatctta gcaatgtggc aaaagtaatt
      601 aaattacctt tgctagatat aggcataaat atttggtcat tatatgattc ttcattaaat
      661 catgctaaag ccaccacaca aattgaatat atatcaacag caatagatgt atctttttca
      721 tctataaata cagctttgtc aataggcgca atcgcatatc ctcctttggc aatagctata
      781 gttccgataa ctattttttc tcatgaagta aaaaactatg ctgtttatgt gaatcaaatt
      841 aatgagcgcc ataagttatg gctcgaagct gaaaagtatc tggacaatgg aagtgcaaag
      901 gttctaagca ttaacaaagc cacaggcata atcgatttat caaataatca ggtgttaggt
      961 aatatttatt tagatatgag agaaaatcct ccaatattac acggtgagaa atcatataat
     1021 agcggaaaga atattggtag tcatcctgat atgactgaca gagagattat ggagtctaga
     1081 gcatacaatt ttgcatgcac aaaactctca gatgcaggtg aacccgatat tttcggttgg
     1141 ggagataagg aaatatgtaa ttcaatggaa ttatcagaat cacaattagc taatgggtat
     1201 tctaaccgcc aatggccttc tcagattcca gttattccag aaggaattta taatacagta
     1261 tatcttggat atggagaaac attgcgagct aatactgaag ttacattgag tagcactggt
     1321 tacttttacg agattgcaag ggcatatact gatgatgaac tatctgagcc attattaaca
     1381 gtatgtaacc aacatagtca tgttattggt ggaaaagaac cattaacaat tattattccc
     1441 gctattgaac atggtatgct tggctctaat ctacatatga ttgaacgttt taaaaactat
     1501 aatttctcga tatcaggagg gaagggaggg ataaaattaa tggttggtgg tattggtgat
     1561 tacaacatag aatgtacccc cggtgttaga aatattatat cgtttgaaca attatcacgt
     1621 gacttcaatc tggatttgga tttatcagat gggaggaaac aaaattttaa ttttcatcat
     1681 ccctcaggtt tttattcagg aaaagttatg agcataacgc aaaagggtat aaatgcggtt
     1741 gttggtacta aactgggata tgataagatt cgtggtaata atcttgataa taccttttct
     1801 cttgggagtg gaggaggtgt tatatattct ggaggaggga gcaataccta tttcgttccc
     1861 gccacactac aggataattt acacatatat atatcagaaa aatcaaatgg caatcatatt
     1921 atattaggtg atatgcactc tcgcctatct attgaatgcc actttagtaa taatgaaaga
     1981 gagtttataa agatgggcta ctataatgga tgtgatgtga ttttagaaag tgacactata
     2041 caaaaaataa aatcatttgc
//
LOCUS       DAZFKY010000360         2046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFKY010000360 DAZFKY010000000
VERSION     DAZFKY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1247
                     /gene="sctN"
                     /locus_tag="ACHKCL_004920"
                     /pseudo
     CDS             1..1247
                     /gene="sctN"
                     /locus_tag="ACHKCL_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1237..1695
                     /locus_tag="ACHKCL_004921"
     CDS             1237..1695
                     /locus_tag="ACHKCL_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO5765701.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1673..1906
                     /locus_tag="ACHKCL_004922"
     CDS             1673..1906
                     /locus_tag="ACHKCL_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765702.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
BASE COUNT          672 a          379 c          481 g          514 t
ORIGIN      
        1 ataaacggct ctctgattga agcagaactt gacgacgttt ctgtgggtga agtatgcgaa
       61 atttatgcga gcaggcaagc aaatgaacgt atagccaggg ctcaggtcgt tggctttcgc
      121 aatgggaaga cattattaaa ccttataggc agctctgtgg ggcttacacg tactgcagtt
      181 ttgaaaccaa cgggagagca gttaaccata cagatcagtg atgcctttct tggttcggtt
      241 ttgaatgcct ctggtcaaat tatggaaagg tttgtcccgg acactccagg cgatagggga
      301 aatttacgcc tgattgatga actgccaccg tcgtatcagg aacgtcgagt tattaataca
      361 ccacttgaga cacggataag agtcattgat ggcgtactga catgtggcat tggtcaacgt
      421 gttgggattt tcgcctcggc tggctgtggc aaaacggttt taatgcatat gctcgtaaac
      481 aatactgaag ctgatgtgtt tgttattggc ttgattggcg aacgtggtag ggaagttacg
      541 gaatgcgcgg aatcgctcaa aaaatcagta aatgcagcga aatgcgtatt ggtctatgcc
      601 acctctgatt tttcgtcagt agaccggtgt aatgccgccc tgatggcgac aaccgtggca
      661 gagtattttc gagatcgagg aaagagagtt gtacttttta tcgactcaat gacgcggtat
      721 gcacgagcac tacgtgatat gaaactggca gcgggtgaac cacctgccag acgtggttat
      781 cctgcttctg tttttgacag tttgcctcgc ttactggaga ggccgggacc aacactaaaa
      841 ggaagtatta ccgagtttta taccgtgtta ctggaagggg aagatgaatc agacccactg
      901 ggtgacgaaa ttcgctcaat ccttgatggc catatctatc taagtcgcaa actcgcgggt
      961 caaggtcact atccggcaat agatgttcta aaaagtgtga gtcgagtatt tggtcaggtc
     1021 actgatgaaa aacacagaga taatgctgca cgggtaagga agaacctcac aactttggaa
     1081 gatctacagg tgtttattga cttaggggaa tatcgcgcag ggcaaatgct gaaaatgatt
     1141 ttgctatgaa tgcccggcct aaactcacaa attggttgaa acagtcagtt aatgaaaaaa
     1201 tgccaatgag tgaaacgcta aaagagcttg aacgaattgt taagtaaagt taatcgactt
     1261 atcagaagaa cagcgcagag cctggctgca tgtgaagcat cattacaaaa gttaaatgca
     1321 gaaaaagaaa agttagccga aaaggaacgc ctgtatgata tgcaacttaa aaacctgcag
     1381 tcactgttag atatgaagga gttactcggc gaagttgtat ttcgccagga tatattttac
     1441 tcgttacgta aagttacagt cattcagcag cagatcgctg agataaatct tgaaaagcaa
     1501 aaaatagccg agcggcgaaa aatacttaac aaggaaatag ttcaacagca agcacaaaga
     1561 aaacactggt ggctaaaagg agaaaaatac gaccgactaa aaaagcgcat caaaaaacaa
     1621 cttttaaatc agatgcttta tcaagacgag cttgaacagg aggaaaaata taatggacga
     1681 agtcaagaaa attgaacatt gtaaaatttt aaacacagac acacccgata attctgcttc
     1741 ggatttagac agtttcttaa agaaaaacaa aaaaaataaa gaaatgagtg agattgtcgc
     1801 tggaaccatt ctccagttat tacgtcagac tcaggataac caattttcta cgttggggaa
     1861 aaagaatggg cacatgaagt ctattgacaa caaatcgtct ctctaggttg acaggaatcc
     1921 atctctttct ttgcaaacaa aaggagccga gcaattttct acaaagaaac ttctcaatat
     1981 gacttcacga gatcagggta ttaattctga actatctaat cgaacgatac aatttaagga
     2041 aaaaat
//
LOCUS       DAZFKY010000361         2041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFKY010000361 DAZFKY010000000
VERSION     DAZFKY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            133..351
                     /gene="ydfC"
                     /locus_tag="ACHKCL_004923"
     CDS             133..351
                     /gene="ydfC"
                     /locus_tag="ACHKCL_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5765703.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            374..781
                     /locus_tag="ACHKCL_004924"
     CDS             374..781
                     /locus_tag="ACHKCL_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765704.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(759..992)
                     /locus_tag="ACHKCL_004925"
     CDS             complement(759..992)
                     /locus_tag="ACHKCL_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765705.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            1466..1654
                     /gene="dicB"
                     /locus_tag="ACHKCL_004926"
     CDS             1466..1654
                     /gene="dicB"
                     /locus_tag="ACHKCL_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5765706.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            1651..1842
                     /locus_tag="ACHKCL_004927"
     CDS             1651..1842
                     /locus_tag="ACHKCL_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5765707.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            1935..>2041
                     /locus_tag="ACHKCL_004928"
     CDS             1935..>2041
                     /locus_tag="ACHKCL_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5765708.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRA"
BASE COUNT          604 a          434 c          465 g          538 t
ORIGIN      
        1 gccaccacat tctgggcaag aaaacttgat tgtattcata accaatttcc tctcgagtaa
       61 cagacccctc agaggatacc acctcgcctg acgtggttaa aaagcaggca acgctaacca
      121 caaggagccg acatgcagaa acgagacccc gtcatcatcg cgccagacta taccgatgat
      181 gaactttatg agtggatgca ccagaaaatt aaggcggtgc aggacctgaa atgggccaat
      241 gaagccaggg ctaagcaggc tgaaaatctg tccgctctgg agcaggatat caccaatctg
      301 gaaaaagcag cggcattaag cattgccaga atgattacat acccacgtta atagctaacc
      361 aacgaggcta ataatggaat ttaaagattt accaacgcca ttacaggaaa tggcatcgaa
      421 tatagttcgt tcacaactgg ctactcttga ccttagtacc gcagaaaaag aaaccatcga
      481 taatatggtt cgtaatgtgc gcaatgcttt ttctgggcta tatggttctg ataatcaaaa
      541 gcaggaaagc gatgttaata aacgggtaat ttctgtttgc gtgaatggcc atgttctttc
      601 atcaatcaaa acagaaacgg cgacagtctt cgattgcctt tgcattgtac agagccttgt
      661 tgatgccctg tttcgttcag tgaatttaga aaatgatgca aatctgcgag ggcgcataat
      721 agcacatcca tatgcacata ctttaggctc tgtggatatc aaagatccca caaatcttta
      781 atgaaatagt taacgcgaat tgtacttgct ctttcggttg ctttcagaat acgcgttgaa
      841 actgctggcg gtaatttggt attccattta ttaaaatcat gcccgggaaa gtactcttcg
      901 aaaatacttt taactgcaga ctcgcctatt gaaatgctgc ttaccatgcg attttgataa
      961 aggcatttag caataagagt tgattttaac attcaccctc ctgagggttg gtaattaagg
     1021 agttctccac gggtgaagtg gagtgcgtgc gccggacacg ggtgaacatc cggcactgac
     1081 agtttactga aaggatattt ctctgaaaag tcagagcata acgcgaaatg gcgcagcggt
     1141 aagtatggcg gggttattcc ttccccgttg aggacaccgg gttgtcaggt tgaccatacg
     1201 cttaagtgac aaccccgctg caacgccctc tgttatcaat tttctggtga cgtttggcgg
     1261 catctgtttg cccatgaact gatgtccgcc ctttttaaag tgaattttgt gatgcagtga
     1321 atgcggctaa gcgcacgcgg aacagttaaa accaaaaaca gtgttatggg gggattctct
     1381 gtatccggcg ttaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcac
     1441 aaaattcatt gttgaggacg cgataatgga aacgttatta ccaaacgtta atacgtctga
     1501 aggttgtttt gaaattggtg tcactatcag taaccctgta tttactgaag atgccattaa
     1561 caagagaaaa cacgaacggg agctattaaa taaaatatgc attctttcaa tgctggcccg
     1621 tttacgtccg atacaaaaag gatgctggca atgaatacag catttgcact tgttctgaca
     1681 gtttttcttg tttccggaga gccagttgat attgcagtca gtgttcacag gacaatgcag
     1741 gagtgtgtga ctgcagcaac cgaacagaaa attcccggta actgttaccc tgtcgataaa
     1801 gttattcacc aggataataa cgaaatcccg gcaggtcttt aaaacagttc cgtaataaac
     1861 atccgatttc attcttatat gccagcaatg gcagggattt gttcaccctt aaatctgtaa
     1921 tgaggtaaaa caaaatgagt aaagtcttta tttgcgccgc cattccggac gaacaggcaa
     1981 taaaggaaga aggtgcagtc gctgtagcca ctgccattga agccggtgat gaacgtcgcg
     2041 c
//
LOCUS       DAZFKY010000362         2001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFKY010000362 DAZFKY010000000
VERSION     DAZFKY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..79)
                     /locus_tag="ACHKCL_004929"
     CDS             complement(<1..79)
                     /locus_tag="ACHKCL_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5765709.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHKCL_004930"
     CDS             complement(91..489)
                     /locus_tag="ACHKCL_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5765710.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHKCL_004931"
     CDS             complement(531..1556)
                     /locus_tag="ACHKCL_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5765711.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHKCL_004932"
     CDS             complement(1612..1944)
                     /locus_tag="ACHKCL_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5765712.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
BASE COUNT          420 a          593 c          540 g          448 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt t
//
LOCUS       DAZFKY010000363         1999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFKY010000363 DAZFKY010000000
VERSION     DAZFKY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..340)
                     /locus_tag="ACHKCL_004933"
     CDS             complement(<1..340)
                     /locus_tag="ACHKCL_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFO5765713.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKA"
     gene            complement(492..863)
                     /locus_tag="ACHKCL_004934"
     CDS             complement(492..863)
                     /locus_tag="ACHKCL_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5765714.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(853..1224)
                     /locus_tag="ACHKCL_004935"
     CDS             complement(853..1224)
                     /locus_tag="ACHKCL_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5765715.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(1237..>1999)
                     /locus_tag="ACHKCL_004936"
     CDS             complement(1237..>1999)
                     /locus_tag="ACHKCL_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5765716.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          564 a          515 c          402 g          518 t
ORIGIN      
        1 gcgctttatc aaaattaaaa tacattgtat agggagtact ttgagggcca acagtaacag
       61 aatttagcac tacaggattg tgaaaccctg gccatagatg gaaaaataat gctatggctg
      121 ataaaactat gccaacttca tatatagatt tagcccaggc gttatatttc cagttgaact
      181 tcaaaataat aaaaaaaacg attgtagcaa caaaaaataa caccgaccaa tcaataatat
      241 cattaagcac agccaggaca acagacaccg tcaacaatga aaaagccact accttatgcc
      301 aactgagagt tgacagtgac agtactaaca caatccacat aagcactcct tttatttaat
      361 gatgaagatt gattatcaat attttcaatt caccaggcaa cattttatct accttccaca
      421 atacatgacc atcaaaaaat aaacatgtta attataaaca cagaaaacat aaccctcatc
      481 actatatatc cctaccgcat atccatatct aaccggacat tcagagccat aagcattcct
      541 tcaataatac cttccgcttt ataaagcctt ttaccaataa gcccatcaga acatctatgc
      601 ttacgtgcaa gagccataaa tgtcattcca cctacgtaat aatccaccaa caaatcgtgc
      661 aaatactgat tattcttgtt taatcgtgcc atacaaccac aaattatcat ggcgtcatca
      721 tcagaacact taggacgtga tttcacttta gtcgggatta atcctttaaa accagcagct
      781 atcgagggcc atgttacatc ttcatgatta tttgctgccc atgcccccca tcgctcaaga
      841 accatctgga tatcacgcgc catcgttacc acctctgatt tcgtaaatct tcacgcccag
      901 ccgtccacct ggcacaggct gaccgcgcac aatattgatt tcatcaaact gctcgtcatc
      961 aatgagcact tccgcatgcg tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc
     1021 acggcggcgc ttatccggtg gttctgcaat aatttttatt gccagccgtc cggacaggct
     1081 taatttcagc cgctgctggc gaacaataag cgccacagcc cggcgataac gctcaccggc
     1141 ttttgataca aaatatgtgc tgccacgacg tcgccagtag gtgttcaccg tcggcgggta
     1201 aggcaaaaca aactctatac gcatcagtaa cctcttttac ccgagcacgc cggttgcaaa
     1261 agcgtgatca agaaaacgaa aaattaaatc aacctgggaa ccatgctttt cttcgaacgc
     1321 cagcggatcc gcatgaagct cgttgtgatg ctcccgacac agcggtagcg tgaaaatatc
     1381 gtgggatttt gttcccattc caccctgacc atgaccaatc agatgatgag gatcgtccgc
     1441 tggcttacca caacacgcac acggctgtgt ctttacccag cgtgtgtatt tctcatttac
     1501 ccagcggcga cgtttaggtc gtttcatgaa ggatttcgga gactccggat caacggcaat
     1561 gctgaccacc gtcttttcct gtggtgggtt ctgttgctgg tgggtgtgag gcggtagcgc
     1621 aatatttttt gtgcgctgct tcagtatgct ggtggcggtc tgttctcccg gtatgatgtc
     1681 gctttcacgg tatacggagc ggattttttc cgccggtaat cccagagaac gacgcgatac
     1741 tgcctcaggt aatgcgtcca ccacctgatt gcagactgcc caccaggata attcagccag
     1801 cgataattcc cgttcctgta tgccattcat tgcgtggcgg atgacgtcaa tcatccaggc
     1861 ggccagattc tgttgagcaa gttgctcaag tgattcggat gtctggtcgc gcagctggtt
     1921 gtcgcagtgc cagcacaaca ccatcgcgcc ggtaccgtaa cgatgtatga cagtttcgct
     1981 gtgatgataa tcaccatga
//
LOCUS       DAZFKY010000364         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFKY010000364 DAZFKY010000000
VERSION     DAZFKY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            299..493
                     /locus_tag="ACHKCL_004937"
     CDS             299..493
                     /locus_tag="ACHKCL_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765717.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            501..1913
                     /gene="pykF"
                     /locus_tag="ACHKCL_004938"
     CDS             501..1913
                     /gene="pykF"
                     /locus_tag="ACHKCL_004938"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5765718.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          540 a          473 c          448 g          484 t
ORIGIN      
        1 caaaaatcaa acaaaatcag acaaataacg cgataaatta ttttaactgt agcaattgag
       61 cgatgatgta tttatacacc ggatgaactt tcacttatcg tcacagtgac aaccccggca
      121 ccagacgttg cgcaaacagc gaagtttttg cgtaaccttt tccctggaac gttaaatctt
      181 tgataacaat ttattgtcta acaagttgta tattttttga aacgctgttt ttgttttcct
      241 tttggattaa tttcagcgta taatgcgcgc caattgactc ttgaatggtt tcagcacttt
      301 ggactgtaga actcaacgac tcaaaaacag gcactcacgt tgggctgaga cacaagcaca
      361 cattcctctg cacgcttttt cgatgtcacc tatccttaga gcgaggcacc accactttcg
      421 taataccgga ttcgctttcc ggcagtgcgc ccagaaagca agtttctccc atccttctca
      481 acttaaagac taagactgtc atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa
      541 ccgaatctga agagatgtta gctaaaatgc tggacgctgg catgaacgtt atgcgtctga
      601 acttctctca tggtgactat gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga
      661 tgagcaaaac tggtaaaacc gccgctatcc tgcttgatac caaaggtccg gaaatccgca
      721 ccatgaaact ggaaggcggt aacgacgttt ctctgaaagc tggtcagacc tttactttca
      781 ctactgataa atctgttatc ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca
      841 caactgacct gtctgttggc aacaccgtac tggttgacga tggtctgatc ggtatggaag
      901 ttactgccat tgaaggtaac aaagttatct gtaaagtgct gaacaacggc gacctgggtg
      961 aaaacaaagg tgtgaacctg cctggcgttt ccattgccct gccagcactg gctgaaaaag
     1021 acaaacagga cctgatcttt ggttgcgaac aaggcgtaga ctttgtcgct gcttccttta
     1081 ttcgtaagcg ttctgacgtt atcgaaatcc gtgagcacct gaaagcgcac ggcggcgaaa
     1141 acatccacat catctccaaa atcgaaaacc aggaaggcct caacaacttc gacgaaatcc
     1201 tcgaagcctc tgacggcatc atggttgcgc gtggcgacct gggtgtagaa atcccggtag
     1261 aagaagttat cttcgcccag aagatgatga tcgaaaaatg tatccgtgca cgtaaagtcg
     1321 ttatcactgc gacccagatg ctggattcca tgatcaaaaa cccacgcccg actcgcgcag
     1381 aagccggtga cgttgcaaac gccatcctcg acggtactga cgcagtgatg ctgtctggtg
     1441 aatccgcaaa aggtaaatac ccgctggaag cggtttctat catggcgacc atctgcgaac
     1501 gtaccgaccg cgtgatgaac agccgtctcg agttcaacaa tgacaaccgt aaactgcgca
     1561 ttaccgaagc ggtatgccgt ggtgccgttg aaactgctga aaaactggat gctccgctga
     1621 tcgtggttgc tacccagggc ggtaaatctg ctcgcgcagt acgtaaatac ttcccggatg
     1681 ccaccatcct ggcactgacc accaacgaaa aaacggctca tcagttggta ctgagcaaag
     1741 gcgttgtgcc gcagctggtt aaagagatca cttctactga tgatttctac cgtctgggta
     1801 aagaactggc tctgcagagc ggtctggcac acaaaggtga cgttgtagtt atggtttctg
     1861 gtgcactggt accgagcggc actactaaca ccgcatctgt tcacgtcctg taatattgct
     1921 tttgtgaatt aatttgtata tcgaa
//
LOCUS       DAZFKY010000365         1903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFKY010000365 DAZFKY010000000
VERSION     DAZFKY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            296..460
                     /locus_tag="ACHKCL_004939"
     CDS             296..460
                     /locus_tag="ACHKCL_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765719.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
     gene            1264..1332
                     /locus_tag="ACHKCL_004940"
                     /pseudo
     CDS             1264..1332
                     /locus_tag="ACHKCL_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            1391..1861
                     /locus_tag="ACHKCL_004941"
     CDS             1391..1861
                     /locus_tag="ACHKCL_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765720.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          634 a          385 c          411 g          473 t
ORIGIN      
        1 atgtagatcc aaatgaaaga tatcaatccg tactagatgt actaaacgat ctctcagcta
       61 taagtgatgg cgttcttgac tggcgtctac agatgacgaa accaactaac ggcacatgcg
      121 aatggcaaaa aaagtctggg gacgctatac tgtctatagt ttttgacgca gaaaattcgt
      181 ctactactgg ttttcgttta tacgatgatg ggcggaaaag gcgtgctacg aacttaacaa
      241 tatcctcagg atgtacccct acaaaactgt ataggttatt aaaggataac tgatcatgaa
      301 aaagcgcgag gaagtaagca agctgcctcg cagacgtgat gcagcattag cggttcccta
      361 caaaaaagat gagttcataa gcccttctga tgacaaaaaa ttttcaaagg cgaaaagttt
      421 tacatctaca tctctaaaag ataaatactt taaaatctag cccggcctca gcgccgggtt
      481 ttctttgcct cacgttcgcc cacctaaaaa acataaccaa ttgtatttat tgatgtaact
      541 cgctaaacca tgcagttatg atccctgccg cataaccttc atcagccaca ttttcaaaaa
      601 taaatttcct tatatatcag aatcatactt cgtagagtta ataaatcacc aaaattcgta
      661 ccaatagttc ttgataatgt cgaactattg gttcattatt atcgtcgtca gcaggacgca
      721 ttactcacca gggcggtgaa tatacaacga ttcgaatatg aatctacggc gctgacaaag
      781 cgcaataacc aaagtgaact ttggggtgtg gtgaagggtt catggacggg aatatgtcgc
      841 acgtaaagcg gcgaggcctg cgggactatt gccgaattga agtaggccga aacaggtcga
      901 aatgggtctc ccacctacca caccaccaaa gttcatcagg aggtctatat gacacgcaga
      961 actcagttca aaggcaattc acgttctcgt cgtcgtgagc gtttaaaggc aaaggcatta
     1021 gctaacggcg tactggcccg cgaagaagca ataagttcag aagtattaca ccgccctact
     1081 ctaagcagag cgcagattca ggctaaaggt actcacgaaa cgcctgagcg catagaagac
     1141 gctaagccaa ttaagttcat ggcacaggac gtgatctggc aacagaaaga atacagacgc
     1201 aatctggagc gagcggccat tgtgtacgcg aatgagtttg gacataagca accagaaact
     1261 ggtgtatgtc ttccaaacgt agccatttac gcggcaggct accggaaatc aaaacaactg
     1321 acggcgaggt gacttgtgtt ggtcgccaga aaatgaaatt aggcagcaaa ccacttattt
     1381 gaggtgagat atgacaaaat catggagcgt accttttcct gaatcagaaa ctgaacatga
     1441 tggaatgcct gttttctgga gattccaggc gacagttgaa gaagatggga taaaaatatt
     1501 cgcacttcaa tatatagctt ttcatcagac agagcattat gcatggttgg ttcctgcgca
     1561 ttggattgtt aattttaaac cagcaccaaa tcagtggtta caggaatgga aacaaaggag
     1621 aaatagatat gcaattaaga aagtagcaaa aaatgcagaa agatcttttg cattcccaac
     1681 gaagaaactt gccattgaaa gtttattgcg ccggaagaaa taccatttaa tgagaatcaa
     1741 acaagatttg gctgttgtat caactcttgt tgatggtatg aagaatattg atacatcaac
     1801 accagatatt gaatataact ttggacacaa ccaagaaaca gaaaattggg tattttatta
     1861 gtacgaataa gcactgtgta ttcattccaa cgagtgaata cac
//
LOCUS       DAZFKY010000366         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFKY010000366 DAZFKY010000000
VERSION     DAZFKY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..414)
                     /locus_tag="ACHKCL_004942"
     CDS             complement(<1..414)
                     /locus_tag="ACHKCL_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO5765721.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQ"
     gene            complement(430..1164)
                     /locus_tag="ACHKCL_004943"
     CDS             complement(430..1164)
                     /locus_tag="ACHKCL_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO5765722.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(1161..1493)
                     /gene="sctI"
                     /locus_tag="ACHKCL_004944"
     CDS             complement(1161..1493)
                     /gene="sctI"
                     /locus_tag="ACHKCL_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5765723.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(1513..1752)
                     /locus_tag="ACHKCL_004945"
     CDS             complement(1513..1752)
                     /locus_tag="ACHKCL_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO5765724.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
BASE COUNT          567 a          362 c          322 g          612 t
ORIGIN      
        1 ttgccttata tagggtaaca acgttgaaaa accacatgtg atgatattgt gatagttagc
       61 gatgccgggt ttgtatcgtg ccttttcatt tatttccagc ggtatagctg ataaataatc
      121 ccttagtaca tcaggaacct tataaaaaaa cccgcgttct gcaaaccttt ctcgataaaa
      181 gtgacaacca ctgagtaaac agatcaatgg aagtaaattc caattgttta tgtagtaaat
      241 aagtgaacta ttcagattaa aatgttcaac tgaaagatta tactgcagga gaatcatttc
      301 attggtaata gatctcagca ccggattgtt aataggtgta ttattcaagc ttactcgttg
      361 aggatgaata taactaattg gagcatatat aatctttctt agggcaagat tcataacgtt
      421 ctcatatgtc tacttaattt tcaacctgac taaattagtt agaatggccc tatacttcca
      481 taacagccag caagtcgcta cggatattaa tgcaagtaag atagaaaccg gcatagcctt
      541 atcataagca aaaacaggtt cgctaatttc atatgttggt gcttgctcaa taatgtctct
      601 tcgttttgac aatacaacag aaatattctc atattgtacg cttgcaaaac tattaacaat
      661 aaatctcttt atgtcattta tttttatttc tggattgata tctttttcat atactgcaag
      721 tacagaaata tgaattggta aagcagtttt accactatcg ccagtatcaa catcgtaact
      781 aacatgtact ctcgaagaaa caataccatc cataattttg agagattgct ctaaccgctg
      841 ctcaatagca gaatatagcc ttgctttttc cgctcgtgga gatgaaacga gtgcatctgc
      901 agggaacatc tgcgatattt gaatatcagg cttacccggg agattgtaga tttttagcca
      961 atccaccgca gaagcaaaat ccgttggttc aacatagatt gagaatccag ttttgccttg
     1021 atccttcttt tcagcattaa tattatgtct ttgtaaaaca gcaaggacat cattagcttg
     1081 ctgttgatca agatggttca gtaattcctg ctgcttgcag ccgcacaaca gcaggataaa
     1141 caataataat gatatatatt tcatgagcgg gtcaacgtct caactgcagc gacaattttt
     1201 cttgctaatg ttcccaccaa tgaaacctcg acattataat taagcacatg atcctgcaag
     1261 actaataatt tttcaggagt taatcctttt ttatcagacg ttaatgcttg taacgcattt
     1321 tgctgccaat tttcagtttg tacagcatag ttagcaaaag cggagatcat tttatcatcc
     1381 gggttgtcaa gattgagaac ccggtcattt aaatcaatca ctttcgcttg ctgtatttca
     1441 tgagcagttg attgctcagc tctatcaata ggtggcatat tgcttactga cataataaaa
     1501 tccttatcca gtttagcgga agttttgtat gattgccgaa tcaagatctt tatacgcttt
     1561 tacaacgtta gattgcgcat ttcgatataa tgtatactca gctaatgcac tttgatattc
     1621 ggcaaggaat ttcgggtcgg aaggattggt tgctaaatcc tccaacgctt tttcaacttg
     1681 ctggttcaga tcatcaacgc cctgatcaaa ttgtttgctg atgtccataa tataaccatt
     1741 ccaatctgcc ataagtcctc ctcaattatt cctgaaatgt ccacttgcta ccttgagact
     1801 tctcgaatcg gttatctccg tagctaaatg aactattatt tgtgtcctga tttttcaaat
     1861 tca
//
LOCUS       DAZFKY010000367         1848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFKY010000367 DAZFKY010000000
VERSION     DAZFKY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            176..>1848
                     /gene="tir"
                     /locus_tag="ACHKCL_004946"
     CDS             176..>1848
                     /gene="tir"
                     /locus_tag="ACHKCL_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO5765725.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          515 a          396 c          477 g          460 t
ORIGIN      
        1 tacccgatgt gtgtttttag cacattattt tcttgcatca aaaatcagat tgtgatttat
       61 ttggtcgatg tcatgttcag ccgtttatcg actacgtgct gtataccata cattattatg
      121 taatagcaaa ataaaggatt gttatctatt tacataaaat aaaaaggaga tatttatgcc
      181 tattggtaat cttggtcata atcccaatgt gaataattca attcctcctg cacctccatt
      241 accttcacaa accgacggtg caggggggcg tggtcagctc attaactcta cggggccgtt
      301 gggatctcgt gcgctattta cgcctgtaag gaattctatg gctgattctg gcgacaatcg
      361 tgccagtgat gttcctggac ttcctgtaaa tccgatgcgc ctggcggcgt ctgagataac
      421 actgaatgat ggatttgaag ttcttcatga tcatggtccg ctcgatactc ttaacaggca
      481 gattggctct tcggtatttc gagttgaaac tcaggaagat ggtaaacata ttgctgtcgg
      541 tcagaggaat ggtgttgaga cctctgttgt tttaagtgat caagagtacg ctcgcttgca
      601 gtccattgat cctgaaggta aagacaaatt tgtatttact ggaggccgtg gtggtgctgg
      661 gcatgctatg gtcaccgttg cttcagatat cacggaagcc cgccaaagga tactggagct
      721 gttagagccc aaagggaccg gggagtccaa aggtgctggg gagtcaaaag gcgttgggga
      781 gttgagggag tcaaatagcg gtgcggaaaa caccacagaa actcagacct caacctcaac
      841 ttccagcctt cgttcagatc ctaaactttg gttggcgttg gggactgttg ctacaggtct
      901 gatagggttg gcggcgacgg gtattgtaca ggcgcttgca ttgacgccgg agccggatag
      961 cccaaccacg accgaccctg atgcagctgc aagtgcaact gaaactgcga caagagatca
     1021 gttaacgaaa gaagcgttcc agaacccaga taatcaaaaa gttaatatcg atgagctcgg
     1081 aaatgcgatt ccgtcagggg tattgaaaga tgatgttgtt gcgaatatag aagagcaggc
     1141 taaagcagca ggcgaagagg ccaaacagca agccattgaa aataatgctc aggcgcaaaa
     1201 aaaatatgat gaacaacaag ctaaacgcca ggaggagctg aaagtttcat cgggggctgg
     1261 ctacggtctt agtggcgcat tgattcttgg tgggggaatt ggtgttgccg tcaccgctgc
     1321 gcttcatcga aaaaatcagc cggtagaaca aacaacaaca acaactacta caactacaac
     1381 tacaagcgca cgtacggtag agaataagcc tgcaaataat acacctgcac agggcaatgt
     1441 agatacccct gggtcagaag ataccatgga gagcagacgt agctcgatgg ctagcacctc
     1501 gtcgactttc tttgacactt ccagcatagg gaccgtgcag aatccgtatg ctgatgttaa
     1561 aacatcgctg catgattcgc aggtgccgac ttctaattct aatacgtctg ttcagaatat
     1621 ggggaataca gattctgttg tatatagcac cattcaacat cctccccggg atactactga
     1681 taacggcgca cggttattag gaaatccaag tgcggggatt caaagcactt atgcgcgtct
     1741 ggcgctaagt ggtggattac gccatgacat gggaggatta acggggggga gtaatagcgc
     1801 tgtgaatact tcgaataacc caccagcgcc gggatcccat cgtttcgt
//
LOCUS       DAZFKY010000368         1818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFKY010000368 DAZFKY010000000
VERSION     DAZFKY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..323
                     /locus_tag="ACHKCL_004947"
     CDS             <1..323
                     /locus_tag="ACHKCL_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765726.1"
                     /translation="SAEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLL
                     KAIYSGCTNLPFSLFINNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKV
                     MFNV"
     gene            316..>1818
                     /locus_tag="ACHKCL_004948"
     CDS             316..>1818
                     /locus_tag="ACHKCL_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5765727.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQ"
BASE COUNT          531 a          285 c          383 g          619 t
ORIGIN      
        1 tcagcgcaga agattactgg atattttttc tgatatcgtt aataaaattt cctgagactg
       61 cagaggaact cctgaacaat gcactggtga cccttcccgc agatgctaat tataaagaca
      121 aaacgttact cctgaaggcg atttacagtg gatgtacaaa tcttccattt tcacttttta
      181 ttaataatga acaattatta gagattaggg agtgttgtaa acaggcaata aaagtaacat
      241 ttgcagcaga gctctttgat acacaaaact gtaacaaaaa acaaaataaa aaaccatgga
      301 agaaggtgat gttcaatgtt taacaaagtt ttggtagggt taaggagtca tccagaatta
      361 ataattcttg gattaatggt gatgattatc gccatgctca taattccatt gcccacctat
      421 ttaatcgact ttttgattgg gcttaactta acattggcaa tacttgtatt tttaggatca
      481 ttctatgttg ataggatctt aagcttttct tcatttccat caatcctcct tatcacaacg
      541 ttatttcgtt tggcgctggc gatcagtacc agtcgactta tacttcttga ggcggatgct
      601 ggagaaatta taacgagttt tggtgagttc gttattggag atagcctggt agttggtttt
      661 gttattttct ccattgttac tattgttcag tttatcgtga ttactaaagg ttcggaacgt
      721 gtggctgaag ttgctgcccg cttttcgctt gacggtatgc caggtaaaca aatgagcatt
      781 gacgccgatc tacgcgccgg gatcattgat gcagatttag caaaagaacg tcgtagtgta
      841 ttagaacgag agagccagtt gtatggttcc tttgatggtg cgatgaaatt tatcaaaggt
      901 gatgctattg caaacattat tattatcttt gttaatatta tcggtgggct atcagtagga
      961 gtaggccaaa atggaatgga tttctcaaca gcgttgactg tctacacaat tcttactgtc
     1021 ggtgatggtc ttgtttctca aattccagct ttattaattg ctattagcgc aggatttatc
     1081 gttacgcgcg taaatggcga tagtgataat atggggcaaa atataatgtc ccagttatta
     1141 agtaattctt ttgtcattgt tgtgacatgt gtcctcgcgc taagtattgg gttgcttcca
     1201 ggttttcccc ttctagtgtt tttatgtttg gcagtaatct taggaattta cttttacttt
     1261 aaattcaaaa agaaagggac tgaagaaacg gttgttgagg gagatattac cgttgggttg
     1321 gaaccctata atcaagatga tgatatctca ttaggtatca ttaataaact tgatcaggtt
     1381 attaccgaaa cggtaccctt ggtcttgatt atgaatagtg tacaggctaa aaaatacact
     1441 gaaatcaatc ttgctgatag gattcgtagt cagtttttta ttgagtatgg tatccgcatt
     1501 cccggtattg tcattcgtga gggggaaggt cttaatgatg aagatgtcat attaatgcta
     1561 aatgaagtca gagcttcaca atttaaaatt tatcatgatc ttgtcttgtt ggttgaatat
     1621 tcagatgaag tggtttcgac tttgatcaag aaacctgtca ttgtaaatag taatggtgaa
     1681 caatattatt gggttacgaa aagtgatgct caaaaattaa caaaaattgg ctgttatact
     1741 cgcacggcca tggatgaaat gtataatcat ttatctgtct gtctggctca taatattaat
     1801 gagtactttg gtatacaa
//
LOCUS       DAZFKY010000369         1787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFKY010000369 DAZFKY010000000
VERSION     DAZFKY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(412..1350)
                     /gene="lrhA"
                     /locus_tag="ACHKCL_004949"
     CDS             complement(412..1350)
                     /gene="lrhA"
                     /locus_tag="ACHKCL_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5765728.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT          489 a          420 c          397 g          481 t
ORIGIN      
        1 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
       61 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
      121 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
      181 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
      241 ttatttgtgc gtacggggcg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
      301 ctgcacgatg tgaagaaggt tctttactaa aaaaaagccg ctggggttta aaacaccccc
      361 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
      421 atccctttca atcaacaatg aatcatcccc ttccggagca gatattgggc tgtattgcca
      481 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
      541 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
      601 cagcacacgc aagtccggac tcatcatctc gaccggccgt gccgtcacgc caagaccggc
      661 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccagggaat
      721 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
      781 tacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
      841 tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
      901 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
      961 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
     1021 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
     1081 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
     1141 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
     1201 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
     1261 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
     1321 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
     1381 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
     1441 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
     1501 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
     1561 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
     1621 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
     1681 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
     1741 aaccttttat ttataagggt cattgcgaat tatctgatgc aaagtta
//
LOCUS       DAZFKY010000370         1777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFKY010000370 DAZFKY010000000
VERSION     DAZFKY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..185
                     /locus_tag="ACHKCL_004950"
     CDS             <1..185
                     /locus_tag="ACHKCL_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765729.1"
                     /translation="EKYNEWARKFSEQIFKITGDENAAKNELEPWTPEGADPNYCWRE
                     VDPVDAANEAMSYHND"
     gene            218..435
                     /locus_tag="ACHKCL_004951"
                     /pseudo
     CDS             218..435
                     /locus_tag="ACHKCL_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            437..700
                     /locus_tag="ACHKCL_004952"
     CDS             437..700
                     /locus_tag="ACHKCL_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765730.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            711..872
                     /locus_tag="ACHKCL_004953"
     CDS             711..872
                     /locus_tag="ACHKCL_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765731.1"
                     /translation="MTTITKERIELFIKNPVENGLTRGEQMELARIALASLEAEPVGD
                     FYEYKPDDW"
     gene            930..1034
                     /locus_tag="ACHKCL_004954"
                     /pseudo
     CDS             930..1034
                     /locus_tag="ACHKCL_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1628..>1777
                     /locus_tag="ACHKCL_004955"
     CDS             1628..>1777
                     /locus_tag="ACHKCL_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5765732.1"
                     /translation="MNVIDLFSGVGGLSLGAARAGFDVKMAVEIDQHAINTHAINFPR
                     SLHVQE"
BASE COUNT          549 a          315 c          445 g          468 t
ORIGIN      
        1 cggaaaaata taacgagtgg gcaagaaaat tctctgagca gatttttaaa attactggcg
       61 atgagaatgc ggcaaaaaat gaattagaac cgtggacgcc tgaaggagcc gacccaaatt
      121 attgctggag ggaggttgat ccagttgatg ctgcaaatga agctatgagt tatcacaacg
      181 attaatgtca ggaggccgcc cgaaagggcg gtaatgaatg gtcacattat ttagaaaaaa
      241 tatccgcgaa agagtagaac aacagaattt ctgtttctca ttctgtttat cgtgttgatg
      301 ataccgatat ccccgttaat cctagtctgg ataatcggaa aaataattga gccagttatt
      361 gaattgtata acgacgtggt atgggcgtca ttcaacacac tgcacaataa aattaatccg
      421 tataaggaaa actgatatgg caactttgac aaaaaaagaa cgggcatggt tgaacgaatt
      481 acaggaagtt cttgatcgct gtccatcacc gaaaaaaatt ggcttttaca ccattggcga
      541 taaaagcatt tacctgtatg acctacgccg catggatgaa atcatggagg ctcttgataa
      601 tcgttcgtcg atggattggt gtgttgctgt tcatgatatg aatgcagggt ttgatgaaaa
      661 gattttgttc ccctcatcag ttgaaagcac tgcgggttaa ggagtaacac atgaccacta
      721 ttaccaaaga acgtattgaa ttgttcatta aaaatccggt tgaaaacggg cttacccgtg
      781 gtgaacaaat ggaactggca cggattgcgc tggcatcgct ggaagcagag ccagttggtg
      841 atttttatga atacaaaccg gatgactggt agcagcgttc ggctggagat aaagcgccaa
      901 aatggacgcc accctatgcc gtttttccag cgcccgtagt gccggaagaa atggatttgc
      961 ttacatgcca tctcgatggt gtaactgaaa catatgctga gggatggaac gcttgccgcg
     1021 ctgccctgct tcagggcaaa ggggagcccg ggaaacaagt tcgcgaattg acaatgctgg
     1081 ttaaacaatt ggttagtcaa ctgaagaaag cgaaaccggg ctgcaaattg ccggataaag
     1141 tgatggacta cctggagcga agcggactta taagcgtgga ggatgtttca cgatgacctg
     1201 gcctgaagca ttcacaacgg taggaattgc gatggcggtg gcgctggttg tgtattcgat
     1261 ttgccgctgg ggataaaaac gatttgcggg aaaaggatag ttaagtagaa ttgctgcggg
     1321 tgcttgaggc tatctgcctt gggcatgaac accaaaggca gatagagaaa aacccacccg
     1381 actataaatc aaagtgtatt acccccattt gttggacgat gaaatgggtt tagttaacta
     1441 tgtcggtatc gacatgacta aaacaacctt aaattgctcg attaatagac aagtagtttt
     1501 gaaagattaa atttatgcga tccggtattt tcaagggata gcctttatga ggttatctta
     1561 tggggttaaa acatcaagaa cagttgtgtt ataatacata gaaactaata agagacgttg
     1621 caactatatg aatgtaatag atttgttttc tggcgttgga ggtctaagtc ttggtgctgc
     1681 acgtgctgga tttgatgtta aaatggcagt tgaaattgat caacatgcta ttaatactca
     1741 cgcaattaat tttccaagaa gtttgcacgt ccaagaa
//
LOCUS       DAZFKY010000371         1728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFKY010000371 DAZFKY010000000
VERSION     DAZFKY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1094
                     /locus_tag="ACHKCL_004956"
     CDS             <1..1094
                     /locus_tag="ACHKCL_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765733.1"
                     /translation="ESDVQGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMAAQ
                     AQAFEEKLASLSAVLQKHVTVDEVHPVLQAMVDDAVGTIPVPRDGRDYDPDVLQQAVN
                     DAVANIPVPADGKSITPDDVRPMLEQMVKEAVSHIPAPRDGRDYDPEVLKQAVNDAVA
                     NIPVPADGKSITPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVRALPA
                     PQDGRDATALEIIPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADID
                     VSMTGERSFTVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCN
                     SMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            1091..1417
                     /locus_tag="ACHKCL_004957"
     CDS             1091..1417
                     /locus_tag="ACHKCL_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5765734.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1427..>1728
                     /locus_tag="ACHKCL_004958"
     CDS             1427..>1728
                     /locus_tag="ACHKCL_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5765735.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDV"
BASE COUNT          380 a          403 c          589 g          356 t
ORIGIN      
        1 gtgaaagcga tgttcagggg gatactgaga aaatgacgga acgggaactg tccattattc
       61 gtgcactggg cgaagaattc tccacggtgc tggcggattt acagcgcaca tttgagggga
      121 agatggccgc gcaggcacaa gcgtttgaag agaaactggc ttccctgtcg gcggtattac
      181 agaagcatgt gacggtggat gaggtacatc cggttctgca ggcgatggtg gatgacgctg
      241 tggggaccat tccggtaccg cgtgatggtc gtgattatga tccggatgta ctgcagcagg
      301 cggtgaatga tgcggttgcg aatattccgg taccggcgga cggcaaaagt atcacccccg
      361 atgatgtgcg tccgatgctt gagcagatgg tgaaagaggc tgtaagccat atccctgctc
      421 cgcgtgacgg tcgtgattat gatccggaag tactgaagca ggcggtgaat gatgcggttg
      481 cgaatattcc ggtaccggcg gacggcaaaa gtattacccc cgatgatgtg cgtccgatgc
      541 ttgaacagat ggtgaaagag gcggtaagcc atattcctgt tccgcgtgat ggtcgtgact
      601 atgatcccga tgttctgcag aaggctgttc tggatgcggt gagagccctg ccggctccgc
      661 aggacgggcg tgatgccacg gcactggaaa taatccccgc cattgacgat caaaaatcct
      721 ttccccgggg cacgtatgcc acacaccagg gcggactctg gcgggcgtat gaaaaaacgc
      781 acgggatgcg gggatgggaa tgcctggttg acggggtggc cgatattgac gtcagcatga
      841 ccggcgaacg gtcgttcact gtggtggtcc ggcagagcag tggccagcgt acggaaaaaa
      901 cattttccct gccggtgatg ctctaccgcg gtgtgttcag agccggtgaa acctaccacc
      961 ccggtgatac ggtgacgtgg gggggctcgc tgtggcactg caacagtatg accggtgata
     1021 aacccggaga agctcattca tcaggctgga ccctggctgc caaacgtgga cgggatgcag
     1081 gaggcggaaa atgacggcat tactgacact ggaagagatc aaggcacatc tgcgtgtcga
     1141 ccatgacgcg gatgatgaca tgctgatgga caaggttcgt caggctaccg ccgtgctgct
     1201 ggcctacatt cagggcagcc gggataaggt gattcgtgag gacggtgagc tgatcccggg
     1261 cgaggcatta acccggatga agggggctgc catgcgactg accgggatgc tgtaccggaa
     1321 tccggatctt gctgagcggg aagacctcgt gcagggggaa ctgccgtttt ctgtgtccgt
     1381 gctgatttac gatttgcgtt gtccgacggt gttatgagga gggggaatgg caatatctgc
     1441 aggtcgtctg acacagatga taagtgttct gaacccggtg ttaacccgta atgctgccgg
     1501 agaaatgacg gaagaatggg tgtcatgcgg gaaaattcat gcggatatcc gtggcaggag
     1561 cagccgggag cggatgcagt ccggtgcgga aatggcgcag gcggaaatcc gcatctgggt
     1621 gcgcggtcag tccggccggg aaatcacggc agcgtcacga cttcatgtgc tgagtggtcc
     1681 atggcgtgac cggatcctga acgttgtcgg gctgcccgtg ccggatgt
//
LOCUS       DAZFKY010000372         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFKY010000372 DAZFKY010000000
VERSION     DAZFKY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..910
                     /locus_tag="ACHKCL_004959"
     CDS             <1..910
                     /locus_tag="ACHKCL_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFO5765736.1"
                     /translation="NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKR
                     RDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSS
                     CQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLF
                     ARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYR
                     IKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPH
                     HYNGGLTPEESENRYHFYCKTVASIT"
     gene            919..1071
                     /locus_tag="ACHKCL_004960"
                     /pseudo
     CDS             919..1071
                     /locus_tag="ACHKCL_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            1161..1535
                     /locus_tag="ACHKCL_004961"
     CDS             1161..1535
                     /locus_tag="ACHKCL_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5765737.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1532..>1723
                     /locus_tag="ACHKCL_004962"
     CDS             1532..>1723
                     /locus_tag="ACHKCL_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5765738.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFT"
BASE COUNT          435 a          455 c          453 g          380 t
ORIGIN      
        1 caaccgcgct cttgatgtcc gactcgctga acggttcacg atagttgcca gactgagtga
       61 cagccactca gttgtcagcc tctgttctgc tctggaaata caccgcagca gttaccagta
      121 ctggcgaaaa cgacgcgata cggttaatcc ggcgcgagtc aggttgtgca gcgaaatacg
      181 ccgggcgtgg aaccaaagta ggggctctgc gggggcgcgc acgctggctg aaatgctgac
      241 ccaaaacggc gtcccgatga gccgttaccg tgccgggcgt ctgatgaaat atctgaacct
      301 gagcagttgt cagcccggaa aacatcagta caaaaatgct cgtcaggaac atacctgcct
      361 gccgaatctg cttgagcgcc agttcgctgt gcccgagcca gatagggtat ggtgcggaga
      421 tattacgtat atctgggcag gaaatcgctg gtgctatctg gcggtcgtca tggatctttt
      481 tgcccgcagg gttatcggct ggagcctgtc agcgaatgcc gatactgccc tgataagcag
      541 tgccctgcgg atggcgtatg aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga
      601 ccagggaagt caatatacag gactgaaata tcaacaactt ctctggcgtt acaggataaa
      661 gcaaagtgtc agccgacggg gaaactgctg ggataacagt ccaatggaac gcttcttccg
      721 cagtctgaaa acagaatggg tgccaaccga tggctacaca ggcaaagacg tggcccggca
      781 gcaaatcagc agttatattc tgaattatta caatagcgtc aggcctcacc attataacgg
      841 agggctgacg ccggaagagt cagagaacag atatcatttt tactgtaaaa ccgtggccag
      901 tattacttga ccactacact ggaactcatg ctcaccagcg gtgagctgaa tccccgccat
      961 cagcataccg tcacgctgta tgcaaaaggg ctgacctgca aagccgatac cctcggcagt
     1021 cgaggttacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta aatcatcaga
     1081 ataaccttct gctccggcct ggtgttttca ccacgccact tccccatttc tcatctctgc
     1141 atatcaggaa aaactttagt atgaaaacat tacccgatac acacgtacgg gaggcatcgc
     1201 gctgcccgtc tcccatcacc atctggcaga cactgcttgg ccgtctgctg gaccagcact
     1261 acggcctcac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag cagcatattg
     1321 aggcaggtat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gtgctggtgc
     1381 gtaccgacca gccgggattc agcgcctgta cccgctctca gttaataaac agcatcgata
     1441 tcctccgggc tcgcagggcg accggcctga tgacccgcga caattacaga acggtaaata
     1501 acattaccct gggtaagcat ccggaggcga aatgatgaaa ctggccctga cactggaagc
     1561 cgacagcgtt aacgtacagg cactgaatat ggggcgaatt gtcgttgaca ttgatggtgt
     1621 taatctcgct gaactgatta acatggtctg cgataacggc tactcccttc gtgttgttga
     1681 tgaatctgac cggacctcag cagactgcac gccaccattt act
//
LOCUS       DAZFKY010000373         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFKY010000373 DAZFKY010000000
VERSION     DAZFKY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1375
                     /locus_tag="ACHKCL_004963"
     CDS             <1..1375
                     /locus_tag="ACHKCL_004963"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5765739.1"
                     /translation="RSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLD
                     GNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAP
                     APKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKF
                     ARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDAL
                     DKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCG
                     WVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQ
                     NASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
BASE COUNT          412 a          436 c          429 g          432 t
ORIGIN      
        1 ccgctcagaa ctactcgatt cacgttttgg cgcaaaggcc atttctacta tcgcggagtc
       61 aaaacgattt ccgctgcacg aaatgcgcga tgatgtcgca ttccagatta tcaatgatga
      121 attatatctt gatggcaacg ctcgtcagaa cctggccact ttctgccaga cctgggacga
      181 cgaaaacgtc cataaattga tggatttgtc gatcaataaa aactggatcg acaaagaaga
      241 atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaac atggttgccg atctgtggca
      301 tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac accattggtt cttccgaggc
      361 ttgtatgctt ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg
      421 caaaccaacg gataaaccaa acctggtgtg cggtccggtg caaatctgct ggcataaatt
      481 cgctcgctac tgggatgtgg agctgcgcga gatccctatg cgccccggtc agttgtttat
      541 ggacccgaaa cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg tgccgacttt
      601 cggcgtgacc tacaccggta actatgagtt cccgcaaccg ctgcacgatg cactggataa
      661 attccaggcc gacaccggta tcgacatcga catgcacatc gacgctgcca gcggtggctt
      721 cctggcaccg ttcgtcgccc cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc
      781 gatcagtgct tcaggccata aattcggtct ggctccgctg ggctgcggct gggtgatctg
      841 gcgtgatgaa gaagcgctgc cgcaggaact ggtgttcaac gttgactacc tcggtggtca
      901 gattggtact ttcgccatca acttctcccg cccggcgggt caggtgattg cgcagtatta
      961 tgaattcctg cgcctcggtc gtgaaggcta taccaaagta cagaacgcct cttaccaggt
     1021 tgccgcttat ctggcggatg aaatcgccaa actggggccg tatgagttca tctgtacggg
     1081 tcgtccggac gaaggcatcc cggcggtttg cttcaaactg aaagagggtg aagatccggg
     1141 atacaccctg tacgacctct ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt
     1201 cactctcggc ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt
     1261 cgaaatggac tttgctgaac tgttgctgga agactacaaa gcctccctga aatatctcag
     1321 cgatcacccg aaactgcagg gtattgccca gcagaacagc tttaaacaca cctgataaca
     1381 taacgttgta aaaaccgaat gcccagcctt taaaaaaaac agctgggcat tcggttgctt
     1441 attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat atgttatatg
     1501 aatgtttatt aaatgtagcg attaccttta atgtcacgac aagggattat ttgcttacta
     1561 ttaatttccc tgcgttctta aacacgtttt tgtattataa atccagtcat cctgcccgta
     1621 gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta tcaatcaatt
     1681 tgacttaaga gggcggcgtg ctacattaa
//
LOCUS       DAZFKY010000374         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFKY010000374 DAZFKY010000000
VERSION     DAZFKY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..132
                     /locus_tag="ACHKCL_004964"
     CDS             <1..132
                     /locus_tag="ACHKCL_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein O"
                     /protein_id="HFO5765740.1"
                     /translation="SPAKLRDKWTQLEINRNKQQAGMIASKPKLDLTNTDWIYGVDL"
     gene            129..830
                     /locus_tag="ACHKCL_004965"
     CDS             129..830
                     /locus_tag="ACHKCL_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765741.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            827..1129
                     /locus_tag="ACHKCL_004966"
     CDS             827..1129
                     /locus_tag="ACHKCL_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5765742.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            1197..1529
                     /gene="emrE"
                     /locus_tag="ACHKCL_004967"
     CDS             1197..1529
                     /gene="emrE"
                     /locus_tag="ACHKCL_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO5765743.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT          443 a          350 c          437 g          412 t
ORIGIN      
        1 agtccggcca aactccgcga caagtggacc cagctcgaaa tcaaccgtaa caagcaacag
       61 gcaggcatga tagccagcaa accaaaactc gacctgacaa acactgactg gatttacggg
      121 gtggatttat gaaaaacatc gccgcacaga tggttaactt tgaccgtgag cagatgcgtc
      181 ggatcgccaa caacatgccg gaacagtacg acgaaaagcc gcaggtacag caggtagcgc
      241 agatcatcaa cggtgtgttc agccagttac tggcaacttt cccggcgagt ctggctaacc
      301 gtgaccagaa cgaactgaac gaaatccgcc gccagtgggt tctggctttc cgggaaaacg
      361 ggatcaccac aatggaacag gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac
      421 catttctgcc atcacccggg cagtttgttg catggtgccg ggaagaagca tccgttatcg
      481 ccggactgcc aaacgtcagc gagctggttg atatggttta cgagtattgc cggaagcgtg
      541 gcctgtatcc ggatgcagag tcttatccgt ggaaatcgaa cgcgcattac tggttggtta
      601 ccaacttgta ccagaacatg cgggccaatg cgctgactga cgcggaatta cggcgcaagg
      661 ctgccgatga actgacctgt atgacagcgc gaattaaccg tggtgagacg atacctgaac
      721 cagtaaaaca acttcctgtt atgggcggta gacctctaaa tcgtgcacag gctctggcga
      781 agatcgcaga aattaaagct aagttcggac tgaaaggagc aagtgtatga cgggcaaaga
      841 ggcaattatt cattacctgg ggacgcataa gagcttctgt gcacaggacg ttgccgcggt
      901 aacaggtgca accgtaacca gcataaatca ggctgcggct aaaatggcgc gggcaggaat
      961 cctggtcgtt gatggtaagg tctggcgaac ggtgtattac cggttcgcta ccagagaaga
     1021 acgggaagga aaggtgagca cgaatctgat ttttaaggag tgtcgccaga gtgccgcgat
     1081 gaaacgggta ttgagggtat ataaaagaac atcaatgggt acacaatgat gaaacaggtg
     1141 agttgagttc aaactgtagt acaattctct ccagtttgaa caggaaagaa tatgttatga
     1201 acccttatat ttatcttggt ggtgcaatac ttgcagaggt cattggtaca accttaatga
     1261 agttttcaga aggttttaca cggttatggc catctgttgg tacaattatt tgttattgtg
     1321 catcattctg gttattagct cagacgctgg cttatattcc tacagggatt gcttatgcta
     1381 tctggtcagg agtcggtatt gtcctgatta gcttactatc atgggggttt ttcggccaac
     1441 ggctggacct gccagccgtt ataggcatga tgttgatttg tgccggtgtg ttggttatta
     1501 atttattgtc acgaagcaca ccacattaaa aataatttct tttaaaagac tgcaatatgg
     1561 cggttcatat ctttacatgg gccgcttttg ttaatgtttt tagtttttgt gtattctttt
     1621 gtgccttcaa gattattgcg ta
//
LOCUS       DAZFKY010000375         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFKY010000375 DAZFKY010000000
VERSION     DAZFKY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            43..183
                     /locus_tag="ACHKCL_004968"
     CDS             43..183
                     /locus_tag="ACHKCL_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765744.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(313..498)
                     /locus_tag="ACHKCL_004969"
     CDS             complement(313..498)
                     /locus_tag="ACHKCL_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5765745.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            540..905
                     /locus_tag="ACHKCL_004970"
     CDS             540..905
                     /locus_tag="ACHKCL_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5765746.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            1195..>1634
                     /locus_tag="ACHKCL_004971"
     CDS             1195..>1634
                     /locus_tag="ACHKCL_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5765747.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTH"
BASE COUNT          381 a          371 c          455 g          427 t
ORIGIN      
        1 tgtaaatctt tagatgagtc gattttgtcc gaggaagttc aaatggattt tatgctgacg
       61 gtttctggtg tggttatcct gtccattgct tatactgcag ataaatatgg ctgccatttg
      121 ttatcacgta ttggcgctta ttgctcgttg atgctgattt tctcgtcgct tttttttgag
      181 taagttatat taattataac aaataatttt ctgtgttttt tcaggctatc ccgtcagacg
      241 ggaagcctgt actgccgggg gacgaatgga aaactgatgt gtccggtaac tgtgtgttct
      301 gtgaacacca tgttacttaa ttatgtaatt catacccgaa cgctctgttg acagccttct
      361 tctgcaggct tcaataaccc acgctgaaaa gttaccggaa cctttatgtt caagggcgat
      421 attgatctgt tcaatcatgt gattggggaa acggatattg cggattgtgg ttctgcgggg
      481 ccggtttttc gatgacattt tctttcctct ggtgacaagc tatatggcga ggattttaca
      541 tggctgtgct tcgtacgtta ccgggcagaa tcaaaactct gaacacccgg cggataaatg
      601 tcctgagggg ggaacagcgt cgtgtcagtg gcagtgcccg ggtttccctc aaacgtcgta
      661 tctggcggag ggatgccgga cactgttgtc tctgtagacg cgtggttgac ctttgtgaca
      721 gtgaactcga tcaccgcatt gcacttcagt tcggtggtgg taatgaggag acgaacctct
      781 ggaccctctg tactgaatgc catcgccaga agtcagcgag tgaagcggcg agtggtatgc
      841 ctgatccgac gttgcctgag cttcctgatg gcacgctcag ggccgacgga atcactggcc
      901 tgtgaccaga cccggggggg atcatccggc gaaaaaaaac gatcgccctg gacaccgcgc
      961 cccctctcac gcagagaaaa aattcccgtt tcagggcagt taacatgtta actggctgcc
     1021 cgggcatttt tgcggttttt atctttatta ttcagtttgt tgtgcggaaa aaatgttaac
     1081 aggctttttc agcaaatgtt aaccaggcag cagttaacat ttgcggcatg agacgccggg
     1141 aaaaatgggc tgaaccatac ccggctgagt gcgttctgga cccgggagga ggctgtgctg
     1201 acaacgcaaa aacgaaaatt tgcgctggcg ctcatgtccg ggaaaaacaa aacagcgtca
     1261 gcccttgccg ccggttattc ggcgaagaca gccagggtta aaggctcgca gctggcaaaa
     1321 gatccggagg tgcttgcgtt tatagcccgt aaacagtgcg agacggtgga ggtggatgag
     1381 gttcctgttt atcggcagaa aaaatcagag ccggaggata aaccccgtcg ccgtgaggcg
     1441 gctgcaatac cacagccgga cgaaacaaat ccggagatgc caccgcccgt ggtgatatct
     1501 cctggtattg agtatatgga ggacggtctt cccgatccgg tgaaagcgat ggggcgtctt
     1561 ctggtggaga acattaatac cgaccccagg ctggcgctgg atgcggctta taagctggcg
     1621 cagttcacgc acca
//
LOCUS       DAZFKY010000376         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFKY010000376 DAZFKY010000000
VERSION     DAZFKY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            76..375
                     /gene="espZ"
                     /locus_tag="ACHKCL_004972"
                     /gene_synonym="sepZ"
     CDS             76..375
                     /gene="espZ"
                     /locus_tag="ACHKCL_004972"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFO5765748.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
     gene            459..836
                     /gene="sctI"
                     /locus_tag="ACHKCL_004973"
     CDS             459..836
                     /gene="sctI"
                     /locus_tag="ACHKCL_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5765749.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            839..1411
                     /gene="escJ"
                     /locus_tag="ACHKCL_004974"
     CDS             839..1411
                     /gene="escJ"
                     /locus_tag="ACHKCL_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO5765750.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1417..>1615
                     /gene="sepD"
                     /locus_tag="ACHKCL_004975"
     CDS             1417..>1615
                     /gene="sepD"
                     /locus_tag="ACHKCL_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO5765751.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDT"
BASE COUNT          539 a          314 c          330 g          432 t
ORIGIN      
        1 taatagttgc ctatgggata atttggttat ttataaccag gtaaatgtct aaattagaca
       61 aaaggagaaa gatcaatgga agcagcaaat ttaagtcctt ctggtgcagt aatgccgctg
      121 gcgacctcac tcagtggaaa taactcagtg gatgagaaga caggagtgat taaaccagaa
      181 aatggaacaa atcgcaccgt tagagttata gccggattag cacttaccac tacggctctg
      241 gcagctctag gtacaggtat tgcagcggca tgctcggaga cgagcagcac agaatactta
      301 gccctgggta ttacttctgg cgtactaggt actcttactg cggttggcgg tgcattagcg
      361 atgaaatatg cctaaccggc ccatcgcata aataaaagat agaggtaatg gatgtattat
      421 gcccttgcct ctttcataaa aagaggctaa cagtaaatat gaacaacatt aatcaatcgg
      481 aaaacattaa tattcaatta aataaagcgc cccaaacaaa tttcgttgat gagcatacct
      541 cgttagcctc agccccctct gcggcaggtg cggctcagtt tcttgatcaa ttactcccta
      601 aaacagcggg agtgtcttct ccagaacaag tgttgattga agaaattaaa aagagacatc
      661 ttgcaacaat gaacagcgat ctcagtttcg atgctctatc tgcaggtggg ctctcgccag
      721 aagacgtgct caccttacaa aagaatgtgc tcaacgcaaa cgtcaacgtt gatgtcgtat
      781 ctaagttagc aagcctcctt tcaacatcgg ttacaaaatt agtttcgatg caataaaaat
      841 gaaaaaacac attaaaaacc tttttttatt ggctgctatt tgtttgacag tggcatgtaa
      901 agagcaattg tatacaggat taacagaaaa agaagctaat cagatgcaag cactgttgct
      961 atccaatgat gtcaatgttt ccaaagaaat ggacaaaagt ggaaatatga cactcagtgt
     1021 ggaaaaagag gattttgttc gcgcaataac gattttaaat aataacggct tcccgaaaaa
     1081 gaaatttgca gatatcgagg ttattttccc acctagccag ttagtggcat cgccatcaca
     1141 agaaaatgca aaaatcaact atctcaaaga acaagacatc gaaaggttgc taagtaaaat
     1201 acctggtgtt atcgattgca gcgtttcatt aaatgttaac aataacgaaa gtcaaccctc
     1261 ctcagcagcc gttttagtca tcagctcgcc agaggttaac ttagcaccat cggtcattca
     1321 gataaaaaac ctagttaaga atagtgttga cgatctcaaa ttagaaaata ttagtgttgt
     1381 tatcaagtca tcctcaggac aggacgggta atatgtatga acaataataa tggcatagca
     1441 aagaatgatt gtgactggct aacggccctt gattttgtaa aggatgtcaa cggttcacca
     1501 acgcatctca ctttttatat ctaccaaaaa aatgctttct tgcacgattt tggaaactac
     1561 tgggttctat atattgagct tagtggtgat tttcgtcagg ttccaacaga tacat
//
LOCUS       DAZFKY010000377         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFKY010000377 DAZFKY010000000
VERSION     DAZFKY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..391)
                     /locus_tag="ACHKCL_004976"
     CDS             complement(<1..391)
                     /locus_tag="ACHKCL_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO5765752.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSLNPVNGLKRIFGLKT"
     gene            complement(400..1167)
                     /gene="sctT"
                     /locus_tag="ACHKCL_004977"
                     /pseudo
     CDS             complement(400..1167)
                     /gene="sctT"
                     /locus_tag="ACHKCL_004977"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1169..1429)
                     /locus_tag="ACHKCL_004978"
     CDS             complement(1169..1429)
                     /locus_tag="ACHKCL_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5765753.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1440..>1613)
                     /locus_tag="ACHKCL_004979"
     CDS             complement(1440..>1613)
                     /locus_tag="ACHKCL_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5765754.1"
                     /translation="VVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSK
                     GLILQYFDLSINP"
BASE COUNT          556 a          334 c          271 g          452 t
ORIGIN      
        1 cggtttttaa gccaaatata cgctttaaac catttactgg attcaatgaa tcaaacttta
       61 attttacagc ttcagttgct aattgcattt tactttgaaa ccaggagaca agtattgttg
      121 tgaacatgca aaccagcaaa aaaggaatga tcgttttcaa ccaaccaatc cccatagctt
      181 taaaatagtc ccaaatatcc ggtttagcgt tatgatcaag gatatattca agaatcgaca
      241 taatgtgatg cacatcaaaa acatatccga gatataaagt acccacaagc attattacag
      301 aaacggttaa gtctctactt tttagaattt gccctttttt agaagcatcc tgcagtttct
      361 tttgtgttgg cttttcagtt ttatttgcca tgaaacactc tatttctcaa tgtatagttg
      421 caattgttct gggaaaaagg ataacggcat cactcgttca gccagtattg ggccaaaata
      481 aataattaat attaaaaatg ccagcgcact tttgacagtc agtgaaatgg caaaagcatt
      541 tagttgtgaa gcatagcgtg ccagcaatcc caggacagcc tcaccaccca gcataacggc
      601 aattacgggg cttgcgtaaa cgatagtatg agtcattata ttattaatat atgaaaataa
      661 aggctctaat ttaggaaaat taaaacttcc tgaaggccat aaattataac tttgctaaag
      721 agtttccaga ataaagttca atccaccgtt agtaagataa accgctgcag agaaaaggtt
      781 aaataatctt gctaattccg aggtatcgac acctgtagcc ggatccaaag tactacttaa
      841 cgttgcaccg cgttggttat cgataaagct acctatagca aggaaaatcc aaaatggaga
      901 tgctaataag caagcaatga acagccctat gataatttct cttgcagcta acaaagcgat
      961 atcgatttca tttaaggtcg ataattcata gtggtaatgc tgaactaatg cacatgatac
     1021 cactattatt attggatgac ggactactgt tggtatattc ccactggcga aaaatggtaa
     1081 caaatagaaa gttggtgcca atcggcaaaa accgagcgct gtagcagcca gtagactatg
     1141 caactgatag agtatcacct cccccatgct atcctctcgc aaatgctaaa ttcagcattt
     1201 caataccaaa agaatataat ttttcaccat accatccgct cattaaaaaa aagcaaatac
     1261 ttacgcacaa taatttcacg ccaaatggca acgtctgttc ttgcaactgc gttaccgttt
     1321 gaaaaagccc taccaataaa ccaacaaatg tcgctactgc aataggtcct gcagacataa
     1381 ctaaaatcag atacagcgcc ctattccctg caaaaacaat gtcatccata tttttttcct
     1441 cagggattaa tggataggtc aaaatattgt aatatgagtc cttttgataa cattgtccaa
     1501 ccgtccatcg cgacaaacag aattaatttt atcggtgtag atatggttac cggactcatc
     1561 atcatcatac ctaacgttaa cagaacgctg gatataacca gatccactac aac
//
LOCUS       DAZFKY010000378         1604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFKY010000378 DAZFKY010000000
VERSION     DAZFKY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(33..>1604)
                     /locus_tag="ACHKCL_004980"
     CDS             complement(33..>1604)
                     /locus_tag="ACHKCL_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5765755.1"
                     /translation="QAAHALAIFKQLRIVDAPGSPTFGESCAPWVFDLVAALFGSYDA
                     QTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNP
                     ARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWL
                     FGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHD
                     PHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEEAFR
                     GFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLL
                     GMYVTGRDRETREWLGWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAE
                     VAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTE
                     RKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMS
                     LNPEPKKKEYAVFFI"
BASE COUNT          342 a          526 c          399 g          337 t
ORIGIN      
        1 agcggttcgt gatggttaca gtgtgaacag ggttatatga aaaaaaccgc atattctttc
       61 tttttcggtt ccgggttaag ggacatcagg gagaccgcat tgaacagcgc catcagcggg
      121 tcaatttttc cccgtccgct ggcctgtttg gtgataagaa tggcgttacc tttaggctcc
      181 acccgggcat taccgacaca ccaggccatc aggggctgat caccgtgaat cagcacccct
      241 tcagccagtt tgcgctcggt ggttttgatg gccccgccca gcttccagcc ctggcttatc
      301 cccaccacac tctcatcggg gatcccggct tccgccagtg aatccagaat ctgccccaca
      361 cctgacgggt caataccgat atgatccagt aactcagcct catgaatgcg acgcacatac
      421 tccgccactt ccgccgtgtc atccccgacc cgacggacaa tcgtcatgtc tccacaggcc
      481 acaaaatcct gaaaccggga tgcctcactc ttccgtctga ccaccgcggt ttcatgcacc
      541 caggcatggc cccagcccag ccattcgcgg gtttccctgt cacggccagt cacgtacatt
      601 cccagcagat catccaggcc cccgccgtca atccccaccg tcaccacatc agcgcgctgc
      661 aggatatcgt ccaggctgac gcgcctgccc tgctgctccc agaaatccgc gcccgcccag
      721 cggtcagaac gcagggcaag accgatttcc acattggcgt gttttgacat gaagccacga
      781 aatgcttcct caccagcctc ccgggcttta cggtactccc ggtacagaaa ggcctcatcc
      841 accgaataac cgagattcgg gttaaccatg gcgaggtttt ccatcagcag gtgagccccg
      901 ctttccacca tttcaggagg atgctcaaaa atcaccggca gaaagtgcgg atcatgaatt
      961 ttgccgtcgc gcacatcccg ggcatactgc agtttctgtc tgaacacccc ggcgggcggt
     1021 tcattcgact gggtggtcgt atacaccaca aacccttccg gacgggaggc aagcccgccg
     1081 atggcttcac gtagcatgtc ttccgccttg tactgcttgc caaacagcca cagttcatca
     1141 atcagagtcc ccacggactt gataccggac accgtattcg gatcggctgc caccaccttc
     1201 agggtggtgt ccgtcacccg atgggtgatg gtccggatat gtgtctgcac ctgacagagg
     1261 tcatccagat catcgtcccg tcgtaccata tccctggcag ggttgaaggc gttagccgcc
     1321 acctccacgg tcggggccag aatggtgtag cccgccgcct gccgccagtt cagtaacagc
     1381 gccgtcatca tgatccccgc agccagcgtg gacttcgagt ttttcttggg gataaggata
     1441 aacacttcct tgatatggcg aacaccggtc tgcgcatcgt aggagccaaa cagggccgcc
     1501 accaggtcaa acacccacgg tgcacaggac tccccgaatg tcgggctacc cggtgcatcc
     1561 acaattcgca gttgtttaaa aatcgccagt gcatgtgcag cctg
//
LOCUS       DAZFKY010000379         1589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFKY010000379 DAZFKY010000000
VERSION     DAZFKY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..132
                     /locus_tag="ACHKCL_004981"
     CDS             <1..132
                     /locus_tag="ACHKCL_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein O"
                     /protein_id="HFO5765756.1"
                     /translation="SPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            129..830
                     /locus_tag="ACHKCL_004982"
     CDS             129..830
                     /locus_tag="ACHKCL_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5765757.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            827..1117
                     /locus_tag="ACHKCL_004983"
     CDS             827..1117
                     /locus_tag="ACHKCL_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5765758.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1188..1466
                     /locus_tag="ACHKCL_004984"
     CDS             1188..1466
                     /locus_tag="ACHKCL_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765759.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
BASE COUNT          461 a          354 c          456 g          318 t
ORIGIN      
        1 agcccggcca aactccgcga taagtggacc caactcgaaa tcaaccgtaa caagcaacag
       61 gcaggcgtga cagccagcaa accaaaactc gacctgacaa acacagactg gatttacggg
      121 gtggatctat gaaaaacatc gccgcacaga tggttaactt tgaccgtgag cagatgcgtc
      181 ggatcgccaa caacatgccg gaacagtacg acgaaaagcc gcaggtacag caggtagcgc
      241 agatcatcaa cggtgtgttc agccagttac tggcaacttt cccggcgagc ctggctaacc
      301 gtgaccagaa cgaagtgaac gaaatccgcc gccagtgggt tctggctttc cgggaaaacg
      361 ggatcacctc gatggaacag gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac
      421 catttcttcc atcacccggg cagtttgttg catggtgccg ggaagaagca tccgttatcg
      481 ccggactgcc aaacgtcagc gagctggttg atatggttta cgagtattgc cggaagcgag
      541 gcctgtatcc ggatgcagag tcttatccgt ggaaatcgaa cgcgcactac tggctggtta
      601 ccaacctgta ccagaacatg cgggccaatg cgctgactga cgcggaatta cgacgcaagg
      661 ctgccgatga actgacctgt atgacagcgc gaattaaccg tggtgaggcg atacctgaac
      721 cagtaaaaca acttcctgtc atgggcggca gacctctaaa tcgtgttcag gcgctggcga
      781 agatcgcaga aattaaagct aagttcggac tgaaaggagc aagtgtatga cgggcaaaga
      841 ggcaattatt cattacctgg ggacgcataa tagcttctgt gcgccggacg ttgccgcgct
      901 aacaggcgca acagtaacca gcataaatca ggccgcagct aaaatggcac gggcaggtct
      961 tctggttatc gaaggtaagg tctggcgaac ggtgtattac cggtttgcta ccagggaaga
     1021 acgggaagga aaggtgagca cgaacctgat ttttaaggag tgtcgccaga gtgcagcgat
     1081 gaaacgggta ttggcggtat atggagttaa aagatgacca tctacatcac tgagctaata
     1141 acaggcctgc tggtaatcgc aggccttttt atttggggga gagggaagtg aacgatagct
     1201 accgacagtt tgaaaactgg tggtcaaaag acaaaagcca gttcacggga gacgatgaat
     1261 taaaagagtt tgcctgggtg atatggcagg catcgcgctc tgctattgaa ctggatatcg
     1321 actggcccga atcgaatgac gacctttgga aagatggtga agaaggtgct tatgcgatgg
     1381 gttatgagga tgggcgtgac aaaacggtaa ttgcagtaat gaaagccatc agggccgcag
     1441 gaatcaaaga aaagaatttc gattaagcaa atatcacttc aataaatcgc ttttaaggca
     1501 tcacaatcgc tctgtggtga ggtaagcacg tgcaaggtat gccgataagc agcgagaatg
     1561 aaaaatgcgt cagaatgcgt ttgaggagg
//
LOCUS       DAZFKY010000380         1557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFKY010000380 DAZFKY010000000
VERSION     DAZFKY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(210..620)
                     /locus_tag="ACHKCL_004985"
     CDS             complement(210..620)
                     /locus_tag="ACHKCL_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5765760.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            906..1112
                     /gene="ybcW"
                     /locus_tag="ACHKCL_004986"
     CDS             906..1112
                     /gene="ybcW"
                     /locus_tag="ACHKCL_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO5765761.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(1277..1471)
                     /locus_tag="ACHKCL_004987"
     CDS             complement(1277..1471)
                     /locus_tag="ACHKCL_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5765762.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
BASE COUNT          436 a          279 c          334 g          508 t
ORIGIN      
        1 tcatattgtt aatatttatt aatgtatgtc agatgcgaag gatcgtcact gtattcccgg
       61 attaactatg tccgcagccc tgacagggaa actcctctgc gggagtgtcc gggaataatt
      121 aataacgatg cacacagggt ttagcgcgta cacgtattgc attatgccaa cgccccggtg
      181 ctgacacgga agaaaccgga cgtgatgatt tagcgtggaa agatttgtgt agtgttctga
      241 atgctctcag taaatagtaa tgaattatca aaggcatagt aatatctttt atgttcgtgg
      301 atatttgtaa cccatcggaa aactcctgct ttagcaagat tttccctgta ttgctgaaat
      361 gtgatttctc ttgatttcaa cctatcatag gacgtttcta taagatgcgt atttcttgag
      421 aatttaacat tcacaacctt tttaagtcct tttattaaca cagtgttatc gttttctaac
      481 acaatgtgaa tattatctgt ggctagatag taaatataat gtgagacatt gtgacgtttt
      541 agttcagaat aaaacaattc acagtttaaa tcttttcgca cttgatcgaa tatttcttta
      601 aaaatggcaa cctgagccat tggtaaaacc ttccatgtga tacgagggcg cgtagtttgc
      661 attatcgttt ttatcgtttc aatctggtct gacctctttg tgttttgttg atgatttatg
      721 tcaaatatta ggaatgtttt caattaatag tattggttgc gtaacaaagt gcggtcctgc
      781 tggcattctg gagggaaata caaccgacag atgtatgtaa ggccaacgtg ctcaaatctt
      841 catacagaaa gatttgaagt aatattttaa ccgctagatg aagagcaagc gcatggagcg
      901 acaaaatgaa taaagaacaa tctgctgatg atccctccgt ggatctgatt cgtgtaaaaa
      961 atatgcttaa tagcaccatt tctatgagtt accctgatgt tgtaattgca tgtatagaac
     1021 ataaggtgtc tctggaagca ttcagggcaa ttgaggcagc gttggtgaag cacgataata
     1081 atatgaagga ttattccctg gtggttgact gatcaccata actgctaatc attcaaacta
     1141 cttaacctgt gacagagcca acacgcagtc tgtcactgtc aggaaagtgg taaaactgca
     1201 actcaattac tgcaatgccc tcgtaattaa gtgaatttac aatatcgtcc tgttcggagg
     1261 gaagaacgcg ggatgttcat tcttcatcac ttttaattga tgtatatgct ctcttttctg
     1321 acgttagcct ccgacggcag gcttcaatga cccaggctga gaaatttccg gacccttttt
     1381 gatcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg ttgcgggttg
     1441 ttgttctgcg ggttctgttc ttcgttgaca tgaggttgcc ccgtattcag tgtcgctgat
     1501 ttgtattgtc tgaagttgtt tttacgttaa gttgatgcgg atcaattaat acgatac
//
LOCUS       DAZFKY010000381         1556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFKY010000381 DAZFKY010000000
VERSION     DAZFKY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..317)
                     /locus_tag="ACHKCL_004988"
     CDS             complement(<1..317)
                     /locus_tag="ACHKCL_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5765763.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLG"
     gene            complement(320..1438)
                     /gene="gmd"
                     /locus_tag="ACHKCL_004989"
     CDS             complement(320..1438)
                     /gene="gmd"
                     /locus_tag="ACHKCL_004989"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5765764.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(1458..>1556)
                     /locus_tag="ACHKCL_004990"
     CDS             complement(1458..>1556)
                     /locus_tag="ACHKCL_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFO5765765.1"
                     /translation="EPTTLIELGLKRRNACADFLLDVVKQEIESRS"
BASE COUNT          481 a          332 c          273 g          470 t
ORIGIN      
        1 ccgaggaaaa gcagtttatt tacattattt ttgtgggcag catgaatgac attcgcctct
       61 atcattatat tctcatatat aaaatcggca ggataagaac tgttagctaa aatacctccg
      121 acttttgctg ctgccaaata aacctggtcg attttctgtg aagaaaaaaa atccaaaaca
      181 gcgctactat ccaacaagtt caattcatcc cgagtacgta aaaccaactc aacatcatca
      241 cgttgtttga ggcgtcgggt aatagctgat ccaaccattc cttggtgacc agcaataaaa
      301 atacgttgct tattcatcat cattccagag ctaagcttac agaaaaacca tgcgatttta
      361 aaagagaatg ttttttagcg gcttcaagat ctttggcaac catttcagaa atcatttcag
      421 caagagtaat ttctggtctc caaccaagtt tgagattagc tttgctcgga tctccaagca
      481 aagtatcaac ttcagctggt cggaaataac gaggatcaac agcaacaatg acatcacctg
      541 gtttcacacc tggagcatcc tgtccttcaa ccgaatctac aatgcctttt tcttcgattc
      601 ctttaccaac aaagctcatc ttaataccaa gttgtgctgc tgccatttcg acaaactgac
      661 ggactgagta ttggactcct gttgcaatca caaaatcttc gggttgctcc tgttgtaaca
      721 tcaaccattg cattctaaca taatcttttg catgtcccca atctcgtaac gaatccatat
      781 tccctaaata caaacatgat tccaagcctt gtgcaatatt tgcaagtcct cgagtaattt
      841 tccttgttac aaacgtttct ccacggcgtg gagattcatg attgaacaat ataccattac
      901 atgcataaat accatatgac tctcgataat ttaccgtgat ccaatatgcg taaagttttg
      961 caactgcata aggggaacga ggataaaaag gggtggattc tttttgaggg atttcctgaa
     1021 caagtccata taattctgag gttgaagctt gatagaaacg cgttttgttt tccaatccta
     1081 aaaagcgaat tgcttccagt aaacgtaatg taccaattgc atcgacatcg gctgtatatt
     1141 ctggagactc aaaagaaact gctacgtgac tcatagcagc taaattatat acttcatctg
     1201 gctgtacctc ctttagaatt ctagtgaggt tagatgaatc agtcagatct ccatagtgca
     1261 agtgaaaatt tgggttagaa ccatgtggat cttgataaat atggtctatg cgttctgtat
     1321 taaaagatga ggctcggcgt ttgataccat gaacttcata ccctttatca agcaaaaact
     1381 cagctagata agatccatct tgtccagtta cacctgtaat aagagcgact ttagtcatta
     1441 tatacctctt gaatatatta agatcgggat tcaatttctt gtttcacaac atctaaaaga
     1501 aaatctgcac acgcattgcg tcttttgaga cccaattcta tcagagttgt aggttc
//
LOCUS       DAZFKY010000382         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFKY010000382 DAZFKY010000000
VERSION     DAZFKY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHKCL_004991"
     CDS             complement(<1..176)
                     /locus_tag="ACHKCL_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765766.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSR"
     gene            complement(240..>1518)
                     /locus_tag="ACHKCL_004992"
     CDS             complement(240..>1518)
                     /locus_tag="ACHKCL_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO5765767.1"
                     /translation="AFNPARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVAADP
                     NTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGV
                     FRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEA
                     FLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQ
                     RADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWAHETAVVRRKSEASRFQDL
                     VACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGI
                     VVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAILITKQASG
                     RGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          333 a          497 c          371 g          317 t
ORIGIN      
        1 cgggaatggt catgctgcca gagaaacggg atcgcactgc caaactccgc gccctccggc
       61 tccaggatgt caccatcccg atccggagaa ggcgttgacg caatcccggt gatcacccgt
      121 tcatcctcac tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca
      181 gcctcctgaa aataaaaaaa ccgccgcagc ggttcatgat ggttacagtg tgaacagggt
      241 tatatgaaaa aaaccgcata ttctttcttt ttcggttccg ggttaaggga catcagggag
      301 accgcattga acagcgccat cagcgggtca atttttcccc gtccactggc ctgtttggtg
      361 ataagaatgg cgttaccttt aggctccacc cgggcattgc cgacacacca ggccatcagg
      421 ggctggtcac catgcaccag caccccttca gccagtttgc gctcggtggt tttaatggcc
      481 ccgcccagtt tccagccctg gcttatcccc accacaattc cgtcggggat cccggcttcc
      541 gccagtgaat ccagaatctg ccccacccct gacgggtcaa taccgatatg gtccagtaac
      601 tcagcctcat gaatgcgacg cacatattcc gccacttccg ccgtgtcatc cccgacacgc
      661 cggacaatgg tcatatctcc acaggcaaca agatcctgaa accgggacgc ctcgctcttc
      721 cgtcggacca ccgcggtttc atgcgcccag gcatggcccc agcccagcca ttcgcgggtt
      781 tccctgtcac ggccagtcac gtacattccc agcagatcat ccaggccccc gccgtcaatc
      841 cccaccgtca ccacatcagc gcgctgcagg atatcgtcca ggctgacgcg cctgccctgc
      901 tgctcccaga aatccgcacc cgcccagcgg tcagaacgca gggcaagacc aatttccaca
      961 ttggcatgtt ttgacatgaa gccacgaaat gcttcctcac cagcctcccg ggctttacgg
     1021 tactcccggt acagaaaagc ctcatccacc gaataaccga gattcgggtt aaccatggcg
     1081 aggttttcca tcagcaggtg agccccgctt tccaccattt caggaggatg ctcaaaaatc
     1141 accggcagaa agtgcggatc atgaattttg ccgtcacgga catcccgggc gtactgcagt
     1201 ttctgtctga acaccccggc tggcggctca ttcgactggg tggtcgtata caccacaaac
     1261 ccttccgggc gggaggcaag gccgcctatg gcttcacgta acatgtcctc cgctttgtac
     1321 tgcttgccaa ataaccacag ttcatcaatc agcgtcccca cggacttgat accggatacc
     1381 gtattcggat cggcagccac caccttcagg gtggtgtccg tcaccctgtg ggtgatggtc
     1441 cggatatgtg tctgcacctg acagaggtca tccagatcat cgtcccgtcg taccatatcc
     1501 ctggcagggt tgaaggcg
//
LOCUS       DAZFKY010000383         1512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFKY010000383 DAZFKY010000000
VERSION     DAZFKY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1512)
                     /locus_tag="ACHKCL_004993"
     CDS             complement(<1..>1512)
                     /locus_tag="ACHKCL_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765768.1"
                     /translation="RRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGT
                     LNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVAADDGSERLVST
                     ARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQ
                     ITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYY
                     FYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDL
                     AEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGR
                     LYIAGIGAGIENTSDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKY
                     LTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGTLNNVTINENCRVLGKLSA
                     NQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGA"
BASE COUNT          309 a          455 c          386 g          362 t
ORIGIN      
        1 agcgccgctg aatgccaccg ccggaataac aatctgccgg tcaaacggct gatcgtcata
       61 aaccctgacg gtaatggtcc ctgatggcca ccgttccggt gcacgggagt cccgggggaa
      121 agctttgccc actgttttaa cgagatcgcc ttcaatctgg ttcgcggaca gttttcccag
      181 aacccgacag ttctcgttaa tcgtgacgtt gttgagcgtc ccggagttcg cattcacact
      241 gccactgata tccgcatttt tagcggtcag ctttccgtct gatgtcaggg aaaatgccgg
      301 aggattgccg ccactggtaa tggtgggagc cgtcagatat ttcaggaaca cttcattcat
      361 gaatatctga tcgccctgac caacaaacat cggctttgtg ttgccattcg caggattaat
      421 catcgcaatc ctgtctgccg ccagcagcac ctgactctgc atgccgtcag aggtgttctc
      481 aataccggca ccgatacccg caatataaag gcgtccgtcc tgcatctgct gcagcttcac
      541 tgcccacatg ctgttcaggt tattatttgt atcaacctga accttctgta tctgctggat
      601 cgctgcactc tggtcttcca gtttcttatt gacggtctgc gtgatttcat tactgacatc
      661 cgtgatggac gttctgattt ccgccaggtc aggcgcaagc tgaccgttat caatctgagt
      721 ccacaactcc tgagccagat gggttttccc tatctctcct ttgaaaaaat ccagatagcc
      781 ggatgcatca tcactcggct ggccaacagc ctccacaaat gccgatttgc caacggtgtt
      841 cacactgcgg atataaaagt aataatcatg gcccggtttg atattgatac tggcggctat
      901 ccagtacagc cccgtgccaa ggtagcgggc tgtggtttca acctgcctga tatcggtaat
      961 ccgcgtttcc gaaaaccaga actcaaactg taccgtcggg tcatacaccg caagacgcgg
     1021 gaccgctgtt atctgaaaat agcccggtgt cagctcaatc tgtgacggcg ctgccggtgc
     1081 ggcaatccgg aacgataccg acgccggatc gccctgctgc ccccacgcat ttaccgcccg
     1141 gactgtcagc ctgtagttcc ccggcgccag ttgcgtgaag cggtatgtgg tttccgtcgt
     1201 ccgggccgtg ctgaccagcc gctcactgcc gtcatccgcg gccacggtca ggcgaagcat
     1261 aaagctcacg cccttcacca ccttcggcgt gtcccatttc gcctgcgcca gatactgacc
     1321 gtcagctgcg ctcacctcca ccgtcaggtg ctgcactgcc ggagggatga cgctgttcag
     1381 ggtgcctgac tgcggctcaa agcgggcacc gttatccacg atggcttctt tttccggtac
     1441 gtgctgcacc gccgtgatgg caaaggtgcc gtccgtgttt tcccggacgg agacacagcg
     1501 gaacaggcga cg
//
LOCUS       DAZFKY010000384         1506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFKY010000384 DAZFKY010000000
VERSION     DAZFKY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            298..459
                     /locus_tag="ACHKCL_004994"
                     /pseudo
     CDS             298..459
                     /locus_tag="ACHKCL_004994"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..508
                     /locus_tag="ACHKCL_004995"
     CDS             443..508
                     /locus_tag="ACHKCL_004995"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO5765769.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            624..881
                     /locus_tag="ACHKCL_004996"
     CDS             624..881
                     /locus_tag="ACHKCL_004996"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5765770.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(928..1497)
                     /gene="nleF"
                     /locus_tag="ACHKCL_004997"
     CDS             complement(928..1497)
                     /gene="nleF"
                     /locus_tag="ACHKCL_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO5765771.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          452 a          347 c          300 g          407 t
ORIGIN      
        1 gaaaaacaac tttttatctt tttgtttcgc tcaaattagt ataaaaagcc gaacaacaaa
       61 caataaaaaa ccattaacat caatagatta tgatggcttc aatctaaata ataggctata
      121 taatactgca agacacaaca catgctgtca ctatgagtca actatttaga tgatattagt
      181 gacctgtttc agagcattag cgcaaggcga ttctttgtct tcttgcacta attttttatc
      241 ataaaaatgt tcctagcact gggcatcaat atcgcaggtc agaaagagct cctggggatg
      301 cggctggccg aaaatgaagg ggcgaatttc tggttcaatg tgctgactga actgaaaaac
      361 cgcggtctga acgatatcct catcgcctgt gtgtatggcc tgaaagaatt cccggaggcc
      421 cgcatccagt tatgcatcgt gcatatggtg cgcaacagca tgcgcttcgt gtcatggaag
      481 gaatacaaag ccgtcactcg cgacctgaaa gcgattagcc tcccacagaa gaggcaggcc
      541 agcaggcact ggaagcgttt gctgcggcct gggactgccg ctatccgcag ataagccggt
      601 gctagctgtc aaactggact aacttggcga cgtttttcgc ttatccggca gatatccgca
      661 aagtgatcta cacaacgaac gccatcgagt cgctgaacag tgtgatccgg catgccatca
      721 ggaaacgcaa ggtgttcccg acggacgagt cagtaaaaaa agtggtgtgg ctggcaatcc
      781 aggccgcgtc acagaaatgg acaatgccgt taagggactg gcgaatggca atgagccgct
      841 ttattatcga gtttggtgac cgtcttgacg gtcactttta agaaaaggca tttacacaga
      901 atcctaaaca ggctcacagg cctctaatca tccacattgt aaagatcctt tgttgtaagt
      961 aagatctggt accctaataa tatcaaaagg attaaaatca tcactgcatc cttccacgag
     1021 gcatttcatt gctcgtagat caccatatag ctctgaagag tgatgcggac atagcagatt
     1081 ataaacttta catttcgaat cttctttgcc ttgtctactt tcctcaattt ttactttcaa
     1141 tgaatgcaga aaaaaaagat ccctgatata actatccctg tcctctatcg tcattcccgc
     1201 cctcagcgct ccgacaacct catcagtatt ttcatttctc acctcaaata cactatttct
     1261 cttatccatc agcccactta caaaagcact tagctcttga aaaatttcct caagctcatt
     1321 attatttctg gcacatgaat atatagagac cataacatca agtttatctt gtaattcagg
     1381 agaaagaaag tgattatgat taagctcact tactgattcc ggagtcgaag ggttacctct
     1441 tcctgataca tacatccatg aataaagatt tgctgaagaa ccacttgttg gtaacatatc
     1501 aaaacc
//
LOCUS       DAZFKY010000385         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFKY010000385 DAZFKY010000000
VERSION     DAZFKY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(113..469)
                     /locus_tag="ACHKCL_004998"
     CDS             complement(113..469)
                     /locus_tag="ACHKCL_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765772.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(677..1237)
                     /locus_tag="ACHKCL_004999"
                     /pseudo
     CDS             complement(677..1237)
                     /locus_tag="ACHKCL_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT          409 a          308 c          347 g          424 t
ORIGIN      
        1 ctacggttaa cggtggttat tttgggaggc gttaacacgt agcggcttgt agttgtaagt
       61 cttgaacagg tacaaagcct gaaatcagga ggatatcgct atcaaacttc ctttatttct
      121 tctcgcagtt tcgcatctta tagaagaatc ctgtatttcc atcttccacg atgaagcgat
      181 cgttaaaagt tggacgacga ataaatgtca ctccacctat tgttgctttt tgttttatac
      241 catcatcaac attttccaga tctggagagt acaattgatc tccatttggc atttgaacga
      301 tgaaattgtt tccattatcg agaactgttg cggattttga cagaatttga gtagcggtat
      361 ccaggctgga aatcctaatg tcacaaacat atttgcctgg ccccagatct tcttctgcaa
      421 atacaggtaa ggaaaataat gcaaatgcga ggatgagatt cttattcatg caaactccag
      481 gaagatcaat aaaagcaata tgttacataa aaagcacgta aatgaaaaga gcgattggtt
      541 aatgagtaat gctattaatc cccgcatggc ctacttttca ttggctgcgg tgttgaacct
      601 cattgaatat gggcaagact ctgactgagt gaccgacgta aataggtaga tgcataatcg
      661 cttaccgccc tgtaactgca aacggtgccc aggcttctgg ctgaatacca cggctttgca
      721 tccacggaac ggcgtgtagc tgctgattga aagggttaac ttcaacctgg tttaccgctg
      781 gaacgacgtt attaaaggcg ataaggtcag ccagtcggtc aggatggaag ttactgacgc
      841 caatagcgcg aattttaccg gcctgttgca gctcttccat ggcacgccag gcaccgtgga
      901 catcaccgta aggctgatga atcaaataca ggtcaacgta atcaagttgc agtcgattca
      961 gagagcgttc gaactgggct ttggcccctt cgtaactggt atcctgcaac cagagtttcg
     1021 tggtgacaaa gagttcgtta cgggcgatac cgctttgttt cagtgcgttc ccgacctggg
     1081 gttcattttg ataggaggcg gcggtgtcaa tcagtcgata accggtgtta atagcgtcaa
     1141 tgaccgctct ttcgcattcg gcggcatcgg tcatctgaaa tacaccaaag cccagcaggg
     1201 gcatttcaat accgttattc agttttactt tttgcatggt gttatccttc cgttatcgtt
     1261 catgagaagc ataacgtaaa gagaagagct cgattagacg gcaggatttg tcaggggtta
     1321 tgaacgaaat tcataaatct gtttgagtgt tgcgatgggt agtgcaagtt cgatatctcc
     1381 gcaatttaca gtccgatgaa ggaaaatgaa tatccataaa aaatatatcg gtttatcctg
     1441 gcgtatatac ctacttcgac gtatttccaa tagttgctaa ttaaaggc
//
LOCUS       DAZFKY010000386         1472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFKY010000386 DAZFKY010000000
VERSION     DAZFKY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1472
                     /locus_tag="ACHKCL_005000"
     CDS             <1..>1472
                     /locus_tag="ACHKCL_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5765773.1"
                     /translation="AHFDGDQSGTLNSVIPPAVQHLTVEVSAADSQYLAQAKWDTPRV
                     VKGVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPA
                     ITTFRINAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSA
                     RYLGSGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREK
                     IGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSATIQQIQRV
                     QKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPES
                     GNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTAKNADISGSVN
                     ANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQK
                     FDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNG"
BASE COUNT          380 a          366 c          443 g          283 t
ORIGIN      
        1 gcgcactttg acggcgacca gagcggcacc ctgaacagcg tcatccctcc ggcagtgcag
       61 cacctgacgg tggaggtgag tgcagctgac agccagtatc tggcgcaggc gaaatgggac
      121 acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc tgaccagtgg aagcggtcag
      181 gacagccgtc tggtgaccac cgccatcact gcggatacag agcatcgttt cagtggtctg
      241 ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca gcagggggaa
      301 ccggccatca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat tgaactgacg
      361 ccggggtatt ttcagataac ggcggtaccg gtgctggcgg tgtatgaccc gacggtgcag
      421 tttgagttct ggttttcgga aaaacgcatc acgaacacgg cacaggtgga aaaatctgcc
      481 cgttatctgg ggagcggcag tcagtggact gtccagggaa gccggattaa gccggggacg
      541 gatttctggt tttacgtgcg cagcgtcaac ctggtgggga aatctgcgtt tgtggaagtc
      601 agcgggcagc ccagcaatga tggtgaaggg tatctggaat ttttccggga aaaaatagga
      661 aaactgcatc tggctcaggg gctatgggag ctgatagaca acagccagct tgcggatgag
      721 atggcggaga tgaagaccac catcacggaa acccgcaatg aaatcacaca gacggtcagt
      781 aaaacgctgg agaaccagag cgccactata cagcagatac agcgcgtgca gaaggacaca
      841 aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga cggcattccc
      901 tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact gagcaacata
      961 ctgctgctgg ctgaccgtat cgcgatgata aatccggaga gcggcaacag cacgccgtta
     1021 tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg actgtttgcg
     1081 gtgagtatca cctcgtccgg caatcccccg acgttttccc tgacgccgga cgggcgactg
     1141 acggcgaaaa atgcggatat cagtggcagt gtgaatgcga actcagggac gctcaacaac
     1201 gtcacgatta atgagaactg tcagattaag gggaaactgt cagccaacca gattgaaggc
     1261 gatattgtca aaacggtcag caagtctttc ccccgcacga gcacttatgc cagtggcacc
     1321 atcacggtaa gaatcagtga tgatcagaag tttgaccggc aggtcatgat accgccagtg
     1381 ttattccgcg gtggtaagca tgagaatttc aacagtaata accaacagtc atactggtat
     1441 tcaacctgcc ggttaagagt gacccgcaat gg
//
LOCUS       DAZFKY010000387         1423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFKY010000387 DAZFKY010000000
VERSION     DAZFKY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            112..456
                     /locus_tag="ACHKCL_005001"
     CDS             112..456
                     /locus_tag="ACHKCL_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO5765774.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            578..850
                     /locus_tag="ACHKCL_005002"
     CDS             578..850
                     /locus_tag="ACHKCL_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765775.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            852..>1423
                     /locus_tag="ACHKCL_005003"
     CDS             852..>1423
                     /locus_tag="ACHKCL_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5765776.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
BASE COUNT          332 a          334 c          399 g          358 t
ORIGIN      
        1 gcgggggagt aatctcccgc cacctctgat gtgtcaggca tcctcaacgc acccgcgctt
       61 taccatactg aaaatgctgt ttgaatgttc atctctgaaa gaggactatg aatgaaaaag
      121 gtattgattg cagcacttat ttccggtgtg tcttttggcg cttttgcaca gcagggtggt
      181 ttccaggggc cagaagcaga gcgttcaaca gtagcgcagg caaaagaact gaaggatgat
      241 gcatgggtta tccttgaagg gagcatcgtt aaaaaagtgg gtgatgaacg ttatgagttt
      301 cgtgacaata gcgggacaat tgtcacggat attgatgaca gcgtatgggc cgggcagaat
      361 gtttctccga aagacaaagt aagaattgag ggtgaaattg ataaagacct gagcagtgtt
      421 gaagttgatg taaaggcact gaaattatta aagtaaccgc ccctgcttgt taagcccgtc
      481 ttactgacgg gttttctgtt tgtacattcc ggcgtattgc cttacaattc gcgcagtcag
      541 cctgaacaac tgacacctgc tgtcaccgga gaatccgatg acacaacaca taaaatccca
      601 caattctgaa gccgacccgg aaattaagca ggggaggcgt tttcgtgcgc ctcagtatgg
      661 ctggtttcac tatctgttct gtacgatcga tgaggcagat atgcttcaag aggcgtatct
      721 gcgtcgcggt gtccgtgtgg agcggagtct gaacgctgat cgtctgacct ggaccgtttc
      781 tgtatatctt cctgttcgtg cacatctgcc acggacacat gcctgctacc gtcagcgcgt
      841 ctggaggtaa tgtgcgggta ttacttcgac ctgttctggt tccggaactc gggctggtgg
      901 tccttaagcc cggtcgtgaa tcattgccag tttttcatcg cggcagggtg ctggtggagc
      961 cggaaccgaa aaacatgcgg gcgctgccat ctggagcggt tcctgctgtt cgccagccgc
     1021 tggcggaaga taaatcactg ctgccatttt tcagcgatga gcgggtgatt cgtgcagctg
     1081 gcggcgctgg tgcactgtct gactggttat tacgtcacgt gaaatcctgc cagtggccac
     1141 acggcgatta tcatcacagc gaaaccgtta ttcacagtta cggtgctggc gcaatggtgt
     1201 tgtgctggca ctgcgacaac cagctgcgcg accagacctc cgaatcactt gagcaactta
     1261 ctcaacaaaa tctgacagcc tggatgattg acgtcatacg ccatgtaatg aatggcacgc
     1321 aggagcggga attatcgctg gctgaattat cctggtgggc agtctgcaat caggtggtgg
     1381 acgcattacc tgaggcagta tcgcgtcgct ctctgggatt acc
//
LOCUS       DAZFKY010000388         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFKY010000388 DAZFKY010000000
VERSION     DAZFKY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..358
                     /locus_tag="ACHKCL_005004"
     CDS             <1..358
                     /locus_tag="ACHKCL_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5765777.1"
                     /translation="LIEPINHFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQR
                     MEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYK
                     PQTTTEAGLRWMDPYR"
     gene            459..1337
                     /gene="glxR"
                     /locus_tag="ACHKCL_005005"
     CDS             459..1337
                     /gene="glxR"
                     /locus_tag="ACHKCL_005005"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5765778.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
BASE COUNT          380 a          305 c          360 g          359 t
ORIGIN      
        1 actgattgaa cctattaacc attttgatat tcctggtttc catctcaccg gaactcggca
       61 ggcgctgaaa ttgattgatg atgttggttg ctgcaatttg aaaattcagt atgacattta
      121 tcacatgcag cggatggaag gtgaattaac caacactatg acgcaatggg ctgataaaat
      181 tggtcacctg caaattgccg ataatccgca tcgcggcgaa ccggggaccg gagaaattaa
      241 ttatgattat ctctttaagg taatcgaaaa ttctgactac aacggttggg ttgggtgtga
      301 atataaaccc caaaccacca cggaagccgg tttacgctgg atggatccgt accgttaaaa
      361 cgtaacgcta ttcagacaat gcttttttag gctgctaagt tggcagggga tcgtgttgtc
      421 tgaattcagg aatagcgaca tttaaaaaga ggttaattat gaaactggga tttattggct
      481 taggcattat gggtactccg atggccatta atctggcgcg tgccggtcat caattacatg
      541 tcacgaccat tggaccggtt gctgatgaat tactgtcact cggtgctgtt aatgtcgaca
      601 ccgcgcgcca ggtaacggaa gcagcggaca tcatttttat tatggtgccg gacacacctc
      661 aggttgaaga agttctgttt ggtgaaaatg gttgtaccaa agcctcgctg aagggcaaaa
      721 ccattgttga tatgagctcc atttcaccga ttgaaactaa acgtttcgct cgtcaggtga
      781 atgaactggg tggtgattat ctcgatgcgc cagtctccgg cggcgaaatc ggcgcgcgtg
      841 aagggacgtt gtcaattatg gttggcggtg acgaagcggt atttgaacgt gttaaaccgc
      901 tgtttgaact gctcggtaaa aatatcaccc ttgtgggcgg taacggcgac ggtcaaacct
      961 gcaaagtggc aaatcagatt atcgtagcgc tcaatattga agcggtctct gaagctctgc
     1021 tgtttgcttc aaaagccggt gcagacccgg tacgtgtgcg ccaggcgctg atgggcggct
     1081 ttgcttcctc acgtattctg gaagttcacg gtgagcgcat gattaaacgc acctttaatc
     1141 caggtttcaa aattgcgctg caccagaaag atctcaacct ggcactgcaa agtgcgaagg
     1201 cactggctct gaacctgcca aacactgcga cctgccagga gttatttaat acctgcgcgg
     1261 caaacggtgg cagccagttg gatcactctg cgttagtgca ggcgctggaa ttaatggcta
     1321 accataaact ggcctgatac ccgcaataaa aatggccgat atcaaaaaat gaatcggcca
     1381 gcaatattaa aaaagaaagc agcc
//
LOCUS       DAZFKY010000389         1400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFKY010000389 DAZFKY010000000
VERSION     DAZFKY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..617
                     /gene="escR"
                     /locus_tag="ACHKCL_005006"
     CDS             <1..617
                     /gene="escR"
                     /locus_tag="ACHKCL_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO5765779.1"
                     /translation="LIIVFFLLSLLPIFVVIGTSFLKISIVLGILKNALGIQQVPPNM
                     ALTSVSLILTMFIMSPIILQINDNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTE
                     KDNVEFFERAAQKKLGNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLI
                     ISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            617..886
                     /gene="escS"
                     /locus_tag="ACHKCL_005007"
     CDS             617..886
                     /gene="escS"
                     /locus_tag="ACHKCL_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO5765780.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            886..>1400
                     /gene="escT"
                     /locus_tag="ACHKCL_005008"
     CDS             886..>1400
                     /gene="escT"
                     /locus_tag="ACHKCL_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO5765781.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFL"
BASE COUNT          406 a          236 c          247 g          511 t
ORIGIN      
        1 ttttaattat tgtatttttt ctgttatcat tactgccaat atttgttgtt attggtactt
       61 cattcctgaa aatttctatt gtactgggga tattgaagaa cgcattaggc attcaacagg
      121 taccaccaaa catggcgcta acatcagtgt ctttgatact gacaatgttt attatgtctc
      181 cgataatatt acagataaat gataatattt ctcaggaacc aatcaattat accgactctg
      241 atttttttca aaaagttgat gagaaaatat tatcaccata tcgcggtttt ttagaaaaaa
      301 atacagagaa agacaatgta gagttttttg aacgtgcagc tcaaaaaaaa ttgggtaatg
      361 aaactatctt aaaaaaagac tctctattta tactgttacc ggctttcacg atggggcagc
      421 ttgaagctgc attcaagata ggttttttgc tctatttacc ctttattgcg atagatttga
      481 tcatttccaa tatcttattg gccttgggta tgatgatggt atcgccagta acaatttcga
      541 ttccttttaa gatcttatta ttcattcttg ttggaggctg gcaaaaatta ttcgaatttc
      601 tgttggtggt gaattgatgg atactggata ttttgttcaa ttatgtgtgc aaacgttctg
      661 gataatattt atcctctcat tgcctacagt catagcggcc tctgttatcg gtattattat
      721 tagtctggtc caggctataa cgcagttaca ggatcaaaca ttgccttttt tgctaaaaat
      781 aatagcagtg tttgctacgc ttgccctgac ttatcactgg atgggaacaa caatcatcaa
      841 cttctcgtcg ataatatttg aaatgattcc gaaggtgaac ggctaatgaa tgagataatg
      901 acggtcatag tatcatcatt ttattgtata ctcaggccgc tggggatgtt tattatccta
      961 ccaatatttt caacaggtgt tttactcagt aactttattc gcaactcaat tatgattgcg
     1021 tttacgcttc cgatcatagt agagaactat acattttcgg aaaaattgcc atctggaatc
     1081 tttcagttga caggaatagc gctaaaagaa ataagtattg gttttttcat tgggttatca
     1141 tttactattc ttttttgggc aatagatgcg gctggacaga ttattgatac tctaagaggc
     1201 tcaacaatat cttcaatttt taacccgtcc ataagtgatt catcttctat cactggcgtt
     1261 atcttgtacc aatttatctc tgtgatcttt gttattcatg gtgggataca aagcatccta
     1321 gataagctat atttatccta cgagatatta ccattacaag ccgatattgc attcaatcgt
     1381 gctttaatag attttttgtt
//
LOCUS       DAZFKY010000390         1396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFKY010000390 DAZFKY010000000
VERSION     DAZFKY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            222..1268
                     /locus_tag="ACHKCL_005009"
     CDS             222..1268
                     /locus_tag="ACHKCL_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5765782.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT          332 a          320 c          382 g          362 t
ORIGIN      
        1 cgttgacgga cctcggggga aaaacgtgta tttttagtca tcctgtttac ctctttctca
       61 gggagtttag tctccaggat tcccggggcg gttcatggtg attgttggta tgacatgaat
      121 aaaaggtttt tcgtttgact ggaaaattat gttgcaggaa cgagaggact gaggaacaaa
      181 gcttattccg gcgctgtccg tccttgctgt cactgacact gatgtctgct ttattatcgc
      241 ctttatctct tcaggcagcg gatgtccggc gtagcggaga tgaagcattt atcattcagc
      301 agcagcgtca ggaagccctt gagcaacaac tgacgccttc agcccctgat gttcgccttt
      361 ctgcacctgg ctcttttgcc cataagatta attttcctgt tgaaacgccc tgttttcaga
      421 ttaaacagac ggaactgaag ggggctgatg cgttaccaca ctggctgcct ttacaaaaaa
      481 tcgccaacgg ggcggtcggg cattgcctgg gggcgaaagg aattaatctg ctgatgagta
      541 cattgcagaa ccgtctggtc gatcatggtt atgtcaccac ccgtgttctg gcaccttcgc
      601 aggatttaaa aagcggtatc ctccggctgg ttattattcc cggtgttgtg cgacatgtgc
      661 gtctgacacc ggacagtgat gactatattc agttgtattc ctcattcccg gcacacgaag
      721 gttctctgct ggatttacgg gacattgagc aggggctgga tttaggtaac agccggatac
      781 agggacaaca tactgagctg aatgcaacca gtggaaatct gtctacacag aatgcgcaac
      841 tgagtgccga tacgctttcc gcccggactg ccgggcagtt cagcagtaat ggcggtacga
      901 taaatgccga cacactgcag atatcggcac aaagcctgtc aaatcgtaaa ggcagtctga
      961 ttcagacggg aacaggggat ttttcgctga gtctgccggg aagcgtggat aaccgggaag
     1021 ggctgcttgc ggcaaatggc gcggtgcgtc tggatgcact gagccttgat aatcgcaagg
     1081 ggaaagtgca ggcggagcag tcaccctccc ttcagaaatc cccgcccacg tttctgaaac
     1141 cgtttgtggc tggtgtctgt gcggcattgc tggcggtcag cgtggctatt ccgggatggc
     1201 agtttctgac acagccatca ccggaggagc agcattttac ctgggggaat ggttgtaaaa
     1261 agcagtgaaa acagtaaata cagagtatcg tgatgaatta gtgctcaggg gctaccagta
     1321 ttcaataacc agcgtatcgg catcgcattc ctgtaattct ggtattgact gttatgacta
     1381 aattcaatgt atatat
//
LOCUS       DAZFKY010000391         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFKY010000391 DAZFKY010000000
VERSION     DAZFKY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..66
                     /locus_tag="ACHKCL_005010"
     CDS             <1..66
                     /locus_tag="ACHKCL_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765783.1"
                     /translation="VSLIHLDASAGCLPGLPTFNW"
     gene            78..275
                     /locus_tag="ACHKCL_005011"
     CDS             78..275
                     /locus_tag="ACHKCL_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5765784.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            360..701
                     /locus_tag="ACHKCL_005012"
     CDS             360..701
                     /locus_tag="ACHKCL_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765785.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            762..905
                     /locus_tag="ACHKCL_005013"
     CDS             762..905
                     /locus_tag="ACHKCL_005013"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765786.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            899..1318
                     /locus_tag="ACHKCL_005014"
                     /pseudo
     CDS             899..1318
                     /locus_tag="ACHKCL_005014"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          405 a          341 c          317 g          321 t
ORIGIN      
        1 gtcagtctca ttcatctgga tgccagtgcc ggatgtctgc caggtttacc cacttttaat
       61 tggtaactag gaacaacatg acatcattaa ccccggaagc agcactggat attctgattg
      121 cgtggctgca ggacaatatc gacagcgaat ccggaattat cttcgacaac gatgaggata
      181 aaacggattc ggcagcattg ttgccctgta tcgaacaggt cagggaagat gtccgtaccc
      241 tgcgccaact gcagcttctg caacagaacc ggtgagtctc actcaccata cttcattcca
      301 ctgacgccag cctgaacagg gctggcgttt tcgtttataa accaaaagga aaaccgataa
      361 tgtcaggcat cataatctcc cgtccggaag tggacaccgg gcatacggac gttatctgct
      421 ccaccagtat ccgacacgtt gtgacggtac ggaatgcagc actgcaacaa accgaaacgc
      481 ttatccggca actggcagaa atctcagtcc tgacagcagc agacattggc ggcaaaaccg
      541 ccctggaccg ggcgatgaaa caggattttc actgcggctg ctggctgatg gagaaacctg
      601 aaaccgcaat gaaagccatc acccgcaacc tcgactgcga aatctggcgt gacctgatgc
      661 aacgctccgg gatgctgagt ctgatggatg cacagggcta ggatacatgg taccgctcac
      721 tggagtacga taatattccg gaaatcagcg aggcgaatat catgaatgca ttcaaacaac
      781 tgcaccagag taaggatgaa gtgtttgagc gtggggtgat caacgtcttc agagggccga
      841 acggaattac aaaaccaatt ccccctgtaa attcggcagt aaaattatcg tcaacaactt
      901 ggtgagatgg gacagatgga gatttcatct tatcagtgga atgcaggcag atcgcttagc
      961 tgacctggaa agaatgttgc atctgctcag cggtaaaccg ctccccaaca accgagggaa
     1021 tatcaccatt aatctggatg accacctaca gtccgttcag ggtaaagagc gctatgaaga
     1081 caagtaaata ctttaagaag ggcccgcaca catcacattc agaaagccag agctggttga
     1141 caggcttaat gatattattg ccagacacta cctaggaatg ttaccttcac aaaaatctct
     1201 aagaattgat tcaattgtgt attttagcct cattccctca tgtaaagtca tttgggggtt
     1261 attatggggg tcaatcattt atccaacacg cattaatcat ttaaaatcaa tgcattaata
     1321 atcatattcg actcctgtga tctccgccaa acttcctctt ctgaactctc ccgacgtcaa
     1381 caaa
//
LOCUS       DAZFKY010000392         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFKY010000392 DAZFKY010000000
VERSION     DAZFKY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..821
                     /locus_tag="ACHKCL_005015"
     CDS             <1..821
                     /locus_tag="ACHKCL_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HFO5765787.1"
                     /translation="QSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            818..>1378
                     /locus_tag="ACHKCL_005016"
     CDS             818..>1378
                     /locus_tag="ACHKCL_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5765788.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          352 a          340 c          397 g          289 t
ORIGIN      
        1 ctcagtccgg attatggggg aaaatttctc aatggtccgg tatcaacaac cagggccggg
       61 aggattacag gaaacgagat tttgccccgg acagccccga aataaaattc agtgatcgca
      121 gggaagccct tgaactgatg acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg
      181 ctgaagcaca gttccggcct gagctggaga aaatcgtcag cgggattgaa acaggttttc
      241 gtggcacggc cctgtacgcc acagaaaaca ttgccggtcg catcaatgcc cgcctggagg
      301 atgagggctt taccgtaaaa atcagttttc cggcagtcag ccagttacag acccggctcg
      361 cggtaaaaat aaatctgagt gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc
      421 gtcggcagag tggcgtatgg ggaaccgttt gtcgatggtt tggcaccagt gacctgggct
      481 gggaaaacta tgacgaggat gtgagtcgca gcgtgatcaa tatcaacaag gccagagagg
      541 aagttatgtc actgacccgg gcatatttcg gggagctgca ggcatccatt gagcaggata
      601 ttaaccagcc cgtccgccag gaaatcgatg cctttttctg cgcattcagg gagaaagttg
      661 aacaactgcg taacacgctg attcagagct ctgaagatca taaacgcgat cagcaggcgc
      721 aggaacggct taccgggcga cttcaggcat taaatgaaag ggttcctgag ctcattactg
      781 acagtaaggc gctgagggaa gagctggaga caatgctgtg acttcaccat ttattcaaca
      841 aattgctgat aaccgggtat gtcaggtact cacctgtctt cctgaaaaat ttgtggttga
      901 tttcgcaaac ggaatcgatg ttgcacagga gcatatccgc acggccggag agcgcacgtt
      961 cttccggcgt ttaaaagagg ggctgaccgg cgaaggcgct gcacggcaga atgccatcaa
     1021 tgcgtcgctt gcacagggcg ttgaggcgtc cctgcgctgg ctgacggaga tgacaacgtc
     1081 acttgccacc acaaactacg cgattacccg ggtaaacgac agggtcagtt cactggtcag
     1141 tgatacagcc aggctggcgc attattccgc agatacgcgg gagcagttac tcaccctggc
     1201 cgatcaggtt caccataaac tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt
     1261 tcagcgggcg cagttacatc ttgagcagat attctcatgg tggagcgccg ggcgatatgc
     1321 gtcattttcc cctgccggac gttgttatgt ggcacttgaa gagcttcgct ggggcgcg
//
LOCUS       DAZFKY010000393         1364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFKY010000393 DAZFKY010000000
VERSION     DAZFKY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            25..1350
                     /gene="nleA"
                     /locus_tag="ACHKCL_005017"
     CDS             25..1350
                     /gene="nleA"
                     /locus_tag="ACHKCL_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO5765789.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          440 a          283 c          282 g          359 t
ORIGIN      
        1 ataaacatgt aataaggatt tattatgaac attcaaccga ccatacaatc tggaatcacc
       61 tcacaaaaca atcaacatca tcaaacagaa caaataccct ctacacaaat accgcaatcc
      121 gaattacctc taggatgcca agctggattt gttgttaata ttccagatga tatacagcaa
      181 catgcaccgg aatgcggtga aacaacagct ctactgagct tgataaaaga taaaggtctg
      241 ctctcagggc tagacgaata tatagctcct caccttgaag aaggatccat aggaaaaaaa
      301 acattggata tgtttggttt attcaatgtt acccaaatgg cattagagat acctagttcc
      361 gtttcaggca tctctggtaa atatggtgtc cagctaaaca ttgtaaaacc agatattcat
      421 cctacatcag gtaattattt tttacagata ttccctctgc atgatgaaat aggttttaat
      481 tttaaagacc ttcctggccc gttaaaaaat gcattaagca acagtaatat atcaaccact
      541 gcagtgtcga ctattgcatc gactggaaca tcagccacta cttcgacggt aaccaccgag
      601 ccaaaagacc caataccatg gtttggatta acagctcaag tggttcgtaa tcatggtgta
      661 gaacttccta tagtcaaaac tgaaaatgga tggaagcttg ttggagaaac accacttact
      721 cctgatgggc cgaaagcaaa ttacacggag gagtgggtta tcagaccggg agaagcagat
      781 tttaaatatg gtgcatctcc attacaggca actctagggc tggagtttgg cgcacatttc
      841 aagtgggatt tagataaccc taatactaaa tatgccgttc ttaccaatgc tgccgcaaat
      901 gcgcttggtg ctttaggggg atttgcagta tccagatttg ctagtacaga tccaatgtta
      961 agtcctcata tcggtgcaat ggttgggcaa gcagcagggc atgccataca gtataatacc
     1021 cctggattaa agccagacac tattttatgg tgggctggtg cgacactggg ggctgccgat
     1081 ttaaacaagg ccgagtttga agtagctaga ttcactgact atcctcgtat atggtggcac
     1141 gcaagagaag gagctatttt ccccaataaa gcagatattg aacatgccac aggtgctgat
     1201 atacgcgcaa tggaagaagg tatccctgtt ggacagcggc atccaaatcc agaggatgtg
     1261 gtaatcgata tcgaaagcaa tggcttacca catcataatc catcaaatca tgttgatatc
     1321 tttgatataa tccaagaaac aagagtctaa gctttaactt gttt
//
LOCUS       DAZFKY010000394         1362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFKY010000394 DAZFKY010000000
VERSION     DAZFKY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..164
                     /gene="cesF"
                     /locus_tag="ACHKCL_005018"
     CDS             <1..164
                     /gene="cesF"
                     /locus_tag="ACHKCL_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO5765790.1"
                     /translation="FFLSLKDDNILLNIAFPADKIDITEFANLMENGYLLKNEIIRSL
                     SSRPTDFLP"
     gene            complement(362..868)
                     /gene="espH"
                     /locus_tag="ACHKCL_005019"
     CDS             complement(362..868)
                     /gene="espH"
                     /locus_tag="ACHKCL_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFO5765791.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(899..>1362)
                     /gene="escQ"
                     /locus_tag="ACHKCL_005020"
     CDS             complement(899..>1362)
                     /gene="escQ"
                     /locus_tag="ACHKCL_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO5765792.1"
                     /translation="NWQLVPHSNALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQ
                     ERRCWIVINTPLATVNLSDNNLSVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHM
                     LRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIVKINEDFYFTVSIVCD"
BASE COUNT          435 a          278 c          226 g          423 t
ORIGIN      
        1 tgtttttcct gtcattaaaa gatgacaaca ttcttttaaa tattgcattt cctgcagata
       61 aaattgatat tacagagttt gctaatttaa tggaaaatgg ttatctatta aagaatgaga
      121 taattagatc cttgtcttcc agacctacag attttttacc ttgataattt ctgcgctgga
      181 tgggatcatc cattccgttt aacatactct tcatcacagg gacctctgat ttctggccgc
      241 taacgtctct gaagattcag cctgtattat ctaaaagcaa tgtaatatca gttaaaaagc
      301 ggaaaaactc cagaattata actattctgg agttaagtca ggaagaaaac tcaaatgcta
      361 atcataatac gctataagag gaagctcttg ttgctatagc cctattatca agacgttcgg
      421 tagactgtac acgacgtaac aaagaagata atcctctttc agctaagatc ccctccaggg
      481 gagtacgagg aaaccaggtt gaagaaagtg gagttatatg tctctcattt aattttcttt
      541 ccaaagcctt agtcacaccg ccagaaaaca cgctaaatag cgttgagaaa catccatttt
      601 ccggtgttat tctttgtact ttgtacacat aatcatcttt tgggccatct ttaataaagt
      661 gaacagcata gtctttgcct ctaaaagaga caatggtgga atcccctatt tctggatata
      721 cccgatgatg gcgttgttgc attcgggctt taagctgaat ttcatctacg atatcttcat
      781 tgggtatatg aagccttctg ttgaacctgt tccatgacgc atgttcactt gtcagaaatg
      841 tcttgaatac cgctcctgat aacgacatga tacatctccc tatataacgt attcctgatt
      901 aatcacatac tatgctaaca gtaaaataaa aatcttcgtt gatttttaca atactcccgt
      961 atgctatcgt cgtattattg cgtattaact gacaaccacc aaaaagatgg tatccatctg
     1021 aattaataat gtcatctttt ttaacattac gcaacatgtg tatatcaata tggactgtac
     1081 cgatcgcaca ataaatgcga ttcatgacag tctcattgct aagagcagat aacagatccg
     1141 cagccagaac atccgtgaca gataaattat tatcactcag atttactgtt gctaaagggg
     1201 tattaataac aatccagcac cttctctctt gactatcatc aacatgatag agtctcagac
     1261 tatcaccaac tctcaactct gtaactggat aatcagtcca cccaggtacg atatagccat
     1321 caaaaaaaag agcattagaa tgtggaacta attgccaatt at
//
LOCUS       DAZFKY010000395         1360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFKY010000395 DAZFKY010000000
VERSION     DAZFKY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            124..795
                     /gene="queE"
                     /locus_tag="ACHKCL_005021"
     CDS             124..795
                     /gene="queE"
                     /locus_tag="ACHKCL_005021"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5765793.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          383 a          279 c          308 g          390 t
ORIGIN      
        1 tttattcttt atgatctaat ccactttttg ccataaacct gcctgtaaca gaatgttaat
       61 aagacatgat tgttgtggca ggatctgcaa taattaacaa ttatccactt cacagagaat
      121 gctatgcagt acccgattaa cgagatgttc cagaccctgc aaggtgaggg ttactttacc
      181 ggcgtccccg ccatttttat tcgtttacag ggatgcccgg ttggctgtgc ctggtgcgac
      241 accaaacaca cctgggaaaa gcttgaggat cgggaagtct cccttttcag cattctggcg
      301 aagaccaaag agagtgataa gtggggggcg gcgagcagtg aagacttgct ggctgtcatt
      361 ggtcgccagg gatataccgc gcggcatgtg gtgattacgg gtggtgagcc ttgcattcat
      421 gatttgctgc cactgactga cctgctcgaa aagaacggtt ttagctgcca gatcgaaacc
      481 agtggtactc atgaagtacg ctgtacaccg aatacctggg ttaccgtatc gccaaagctg
      541 aacatgcgcg gcggctatga agtgttgtca caggcactgg agcgagccaa cgaaatcaag
      601 catccagtgg gacgcgtacg tgatattgaa gcactggatg aacttttggc gacgctgacc
      661 gatgataaac cgcgagtcat tgcactgcag ccgattagcc aaaaggatga tgccacacgt
      721 ttgtgcattg aaacctgcat tgcgcgtaat tggcgtttgt cgatgcaaac ccataaatat
      781 ttaaatattg cctgattaaa catttataag cgttataaat gggtggaacc tgtaaagact
      841 tctactcatt tatattcttt gtcgtctctg aaaactcatc catcttgcct atcccggact
      901 aatcattatc attctctaca aattctgtgg cgttaatttt tcgctggagt gaaaattatt
      961 gcggtaaagt ttggtagatt ttagtttgta tagagttatt ttaaatattt acctttttaa
     1021 tcaatgggtt aagtgctctt taacataatg gatgtgttgt ttgtgtgata ctgtaaagtt
     1081 ggtagattgt gactggctta aaaaatcatt aattaataat aggttatgtt tagagagttc
     1141 cccgcgccag cggggataaa ccgcaaaagc gggcagcgaa aaaattaaag tcgaggagtt
     1201 ccccgcgcca gtggggataa accaccatat aacccgttat ctctttctca agtttttata
     1261 ttagcagtac tcgtaataag caacatatcc acgtaacccc tcatgttcaa aatagctctc
     1321 catatatgaa aagttcacaa ttatcgatac aaaaaatcaa
//
LOCUS       DAZFKY010000396         1358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFKY010000396 DAZFKY010000000
VERSION     DAZFKY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            512..703
                     /locus_tag="ACHKCL_005022"
     CDS             512..703
                     /locus_tag="ACHKCL_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765794.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(773..913)
                     /gene="ykgO"
                     /locus_tag="ACHKCL_005023"
     CDS             complement(773..913)
                     /gene="ykgO"
                     /locus_tag="ACHKCL_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5765795.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(913..1176)
                     /gene="ykgM"
                     /locus_tag="ACHKCL_005024"
     CDS             complement(913..1176)
                     /gene="ykgM"
                     /locus_tag="ACHKCL_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5765796.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          401 a          279 c          297 g          381 t
ORIGIN      
        1 gcaatataac gttcaattct tacttgcatt ttatattagc caggaacttt gccctgaggt
       61 gttaggaata gtcttaatgt gttgtgtaat ggactagtaa aaaaacaagt ctaaatgata
      121 agaaatagaa tatgaaacta tcatgagtca taattaaaga tgatttgcca atcagtaagg
      181 tgttttaatg gattaacatg ccgcacaaaa tggttatggg tgttcatccc aataagtgat
      241 gaaaaattaa acaggtgtaa gtacgtcatc tgtccggtaa gccccctaag agccagatta
      301 agtgaagtta aacgaagact gccagttgtc accggaacga tggcctcttt gccagggcac
      361 tgcattcagt catctgtgcg cgttactcta actggcactc gaacgtcggc gacggggtta
      421 ccggtggatc aggccgttcc tgtggcgtaa acgtttttat gtcagtacca acagatttac
      481 cacatttatc atcttaacag tttgagtgta aatgcaggct gcgtcgcaaa gggaggaaac
      541 tgagtggcgt gttcagtcta aaagagggtt gatgccagca tacaggggcg aagctggtca
      601 acaagtcaac attaatataa tggaatattc ggagagaaac gtcaggcagt tgaccttaaa
      661 tgaacaggaa gatacatccc caggaaaatt aatgctggtg taataaatac accaacatta
      721 atccggtctg attattaatc agacaaatat cgttaattcg tcgaatttta aatcaacgtt
      781 ttttcttacg accctgaacg gccttaaagc gtggattaga tttacaaatc acatatagcc
      841 gtccttttcg cttcacaatc tgacagtctg gatggcgttc ttttgcggta cgcagagagt
      901 taaggacttt catcacgccc cctttttcgt gctaacaaaa cgaccaaaac gttgggtgaa
      961 tcgtgcaaca tttccttctg atgccactgt tctcagcttc cctgtataga acgggtgcga
     1021 tttcgaagag acatcaattg ttacgtacgg atacgttacg ccatccagct caatctcacg
     1081 gtctgttttg atagtcgagc cgattttaaa gtactcatca acactggtgt cgtggaacac
     1141 cacagtacga tactcaggat ggatattggg cttcatcatt tttacctgtt atgttataac
     1201 ataaccacaa agatacatgc tcatccggat catttcaaca cccaatccct gtgaccatta
     1261 gtggtatgtg gaatttcctc aacggggaag tctggcattg ttaacagcca ttggcgacgc
     1321 tcccataaac gcctggatga gtgattggta agcgtaca
//
LOCUS       DAZFKY010000397         1356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFKY010000397 DAZFKY010000000
VERSION     DAZFKY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1121)
                     /locus_tag="ACHKCL_005025"
     CDS             complement(<1..1121)
                     /locus_tag="ACHKCL_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5765797.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAA"
     gene            complement(1169..>1356)
                     /locus_tag="ACHKCL_005026"
     CDS             complement(1169..>1356)
                     /locus_tag="ACHKCL_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5765798.1"
                     /translation="SPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCWGEDEDKPE
                     VDPFAALEDALSLAAQS"
BASE COUNT          277 a          466 c          321 g          292 t
ORIGIN      
        1 tctgcagcaa tctgcatctc cgtcccgcgg gctgccgcca cggcctgctg agcacggtac
       61 acggctgccc ttgcccgcgc cgtggcaatc tgcgttcccc tgaactgtgc ttccgccagt
      121 gcaacttcat tacgtgcagc cgtcacaagt cctgccgtgg cagacatcgc tccggaggcc
      181 atattgccaa agtaccgggc aaccccgacg gcaaccagcg cgcccacggc tgttgccaca
      241 ttatcaatct gtccggcaac accgttcagc atgccggaga gcgtttttgt caccccgctg
      301 gcctcattcg caccgcccac ccaggccata aaggcgtttt ccacctttgt gatactactg
      361 gaaaccgttt ccggcatggc cgcatattca tcacgtaata tccccagctg gctgattaac
      421 gcggggacca ctttatccgc tgtcagtttt ccgtcatccg ccattgcctt cagatcttta
      481 cgggccacgc ccatacccgc agccagtgca cgtacgatcc ggtcaccact ttcattgacc
      541 gaattaaact cctcaccacg caatacaccc tgcgccagcg cctgactgaa ctgggtgatc
      601 accgagcccg cctctgccgt actggcaccg gagattttca gccctgtcga aatggcctcc
      661 gtcaccttca gcacatcatc agcactgtaa ccatattcac gcatcgaggc agccgaacgg
      721 gcaaacaggg ccgcattatc cgaaaatgcg gtgcctgtcc gctggctgat atccatcagc
      781 actttctgtg atgacgcaaa ttcatcggat gactgtgatg cctgtttcag acgggcattc
      841 acggagctcc attcatccgc cagcgaaatc aggtgtccgg tggcaaaggc accggcaaac
      901 gcaccggtca ttccgacagc cgaagcgcgg atttccgtca actggctgtg cagctcagcc
      961 agagcccggc gctgctccct ggctgccgca gcagcctgac gtccgccatt ctgcagggtc
     1021 cggtaatatt cactgcccat acgggacgcc cgctggatct ccgactggaa tgactgtgaa
     1081 tttgccgaaa ttttgataat cagttcacgt aacgtcgcca ttcacctttc tccgggcgta
     1141 aaaaaaccgc ctcagcggtt ctcatcattc atgactgtgc tgcaaggctc agcgcgtctt
     1201 ccagcgccgc aaacggatcc acctccggct tatcctcatc ctcgccccag cagagcatgg
     1261 cgtccttcag tgcaacattc atcccctgtg ccccgaaaac cgctttcacg atctgtgcat
     1321 tacggatatc cccgcgctca tcacccagcg gggata
//
LOCUS       DAZFKY010000398         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFKY010000398 DAZFKY010000000
VERSION     DAZFKY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            270..773
                     /locus_tag="ACHKCL_005027"
     CDS             270..773
                     /locus_tag="ACHKCL_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5765799.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(853..1104)
                     /gene="yecJ"
                     /locus_tag="ACHKCL_005028"
     CDS             complement(853..1104)
                     /gene="yecJ"
                     /locus_tag="ACHKCL_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5765800.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1219..1305)
                     /gene="azuC"
                     /locus_tag="ACHKCL_005029"
     CDS             complement(1219..1305)
                     /gene="azuC"
                     /locus_tag="ACHKCL_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5765801.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          376 a          301 c          285 g          379 t
ORIGIN      
        1 tgattaatat caacaagata caaaaagcac tatcattaaa attcattgca gttacattga
       61 tttcatcaat gaaatgtaaa aatatataaa cttgatgatt taagcatttt cttatacccg
      121 ttcagacgtt attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga
      181 ccggcagaaa ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct
      241 ctccacttct ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca
      301 actctcaaat gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt
      361 gttctgaaca gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg
      421 tgacccaaat gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta
      481 aagccattga tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga
      541 tggaagaata cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac
      601 tgaatgatga ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg
      661 gcctgttgct gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc
      721 cagtgcagac cgaccaacat gttctgaatg tcgtgtcgca tcagctgcat tgatcataca
      781 tcgacgctaa tgcattgcac cgatgaagtt tttgagaaac cgctgcctca tcttttttga
      841 agcagcggtt ttttaatggg attcgccttg tggggtaaac ttgagctcaa tcagcgcgat
      901 ggctttttgg attgcccgca tggtgaccgg gtctgcggca gcgggatgat gagtaaagtc
      961 gatattcttc agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag
     1021 aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg
     1081 atcggcattc agcgtctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag
     1141 tagcggcttc acatcttcaa cgatagtcac atccaccctc atcaggacac aaaaaaacct
     1201 gccggagcag gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt
     1261 gcaatagtta ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc
     1321 atttgttata caggtgttat t
//
LOCUS       DAZFKY010000399         1328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFKY010000399 DAZFKY010000000
VERSION     DAZFKY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            309..>1328
                     /locus_tag="ACHKCL_005030"
     CDS             309..>1328
                     /locus_tag="ACHKCL_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO5765802.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQ"
BASE COUNT          443 a          219 c          249 g          417 t
ORIGIN      
        1 tttagttatt ggctgtgttt atgagaactg acacataaag aatattaaca aaaatacgtg
       61 aatggataat ttttttaatt tttataatta ttgttgaaga tgatgatatt gattataggg
      121 ataccattac aaggagctgt tcccctcgtt gatgtcgcgg tttacccacc tcattaccag
      181 gttgatgacc tgatggggaa atgacgtaaa aagacaaata actctataaa tgagcgaaat
      241 ttgatagttg atttttaaat agtaacctta ttattactac aactttcata tggatgaggg
      301 gaaagaaaat ggttgataac gtaactgtta gcagagtatg tatacaatct ccttctttcg
      361 tgcctgattt ggatggagaa aaaaataaat ctcaattatt cgttgacgat atagttgcgt
      421 atcttaaaag tccttcagtt tattcacttg aaaaagaggg gccgttaaat cattttgtta
      481 atcattgttc tgaagttgag ttaggtttct atagcgatgg tgcatattca attcttgtct
      541 ccagatctaa gcaacaacct gaaggtatga ttttaaccgt aagcgatgca gacgcaatca
      601 atatagtaca tatttccgta tctccagtgc ttataaaatt cctggatgat atttttactt
      661 gccttcatac gtaccctgat gatgagagtt ttacaaaaga gcagataaaa gctaatagca
      721 aatatgatat tgtagattat aattgcctgt tgcattttac tggaaaacca aaaagtttaa
      781 tagaatgtag acattttgct ctgcaatact gtatagattc aatgaatgag catacaggga
      841 aagttccatt aaaggcttac tattcatctc cggaagatat acaaaaacat attcctttcg
      901 agcttgagca gcaatttaac aatctacaaa aaaatccacc acccggtaca tgcgtcgttg
      961 ccagtgataa gtttggggag gcattatctg tcttttttca cagaatggaa aaagagaagt
     1021 taacgcatat gacggcaatc gttcaatctc aaacacacgc tatggccgtc cgcttgagga
     1081 tcaaaaaaac gcctgctggt gaaacagaat atgttgtatc cttttatgat cctaatgcaa
     1141 ccaatactgc agtacgctat aaagcaaaca actgtgattc ttttgggtca ttgcaatcgt
     1201 ttataaatat tcaacaggca aaacaaaaat gggtaataac agatatttgc tccgagtgtg
     1261 taggaataac cccttatctc cctcgggaac aagcccattt attaagtggt attgaaaatg
     1321 agttgcaa
//
LOCUS       DAZFKY010000400         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFKY010000400 DAZFKY010000000
VERSION     DAZFKY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..103
                     /locus_tag="ACHKCL_005031"
     CDS             <1..103
                     /locus_tag="ACHKCL_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO5765803.1"
                     /translation="EFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            727..1248
                     /locus_tag="ACHKCL_005032"
                     /pseudo
     CDS             727..1248
                     /locus_tag="ACHKCL_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
BASE COUNT          459 a          210 c          259 g          396 t
ORIGIN      
        1 ggaattcgct gtaaataacg gtaatattga tgcaaagagc aaacttcaag agctcaaaaa
       61 gctaaagcga atggatagaa ggaagaataa ggagaatccg taaaagcaat agttcatcag
      121 actgccgcag cagtagtatg atgatgaatc agggctgtac tataactatt atcggtaaca
      181 ttaaccgcag caggggcggt ataacactca ggctccgatt gggctggaag gtaggtggaa
      241 tttttatcag tatccgttga acccgatttc tggaatcgat ccccttggga ttagccacct
      301 gtttatacag cataacttta ggtatgcttt cgtgtgtttc agatatacca tgtgatgata
      361 atttttatga tgtgcttagt attccagtcg cttctggtaa taatatgcaa tgtaaaaata
      421 atccccgttg tacacattta taaatagatg gccaatatct caaggcctat ggagttggaa
      481 tgttaatgac ccgggagcaa gcaatagaaa acctgacggc atacgattag taccgtcagt
      541 taatacagga acttataatc gcaatggatt ttcaatacat agttgtttaa acgcttttgg
      601 tccgtcttta ggacctcgat tctgttccga gggttgtata actggtttat ctaatgacat
      661 gcaaaaatta aatgagttaa tattttcaga gccagatagc acattaacag ttacggacta
      721 aaaaatatgg caaggttatt aacaattgta tttttttctc tatttctgcg agtgcagtta
      781 ctgcaagaca atttcagaga gagttagatt tggcagacaa agcaatatta tgggcactaa
      841 tatcagccag tactaaagag ggacgaaaag cgtgttcact aagctatttt gcctgtaagg
      901 ccgcagaggc tgaactcgga ctggcatata tggctgcgaa tgataacaag gaatttctca
      961 catccttatc taatataatg agatacaaaa tagatgctgg actttccgaa tcctatacat
     1021 gctatttatt aagtaaggga aagatcataa gaccatatct gaaaaatcta aatccactcc
     1081 aacttgccgc tgattgcatt gaaacagtaa ataaaataaa agataagaat aaaaaaaatc
     1141 attgatataa attcagttaa tatttgtagt gatgataaaa atataaaatt gagagttaat
     1201 agtactatta tggccattga tgattctata aaatgcatcg atgaatgata acttaatttt
     1261 atttaaaaat atattaataa ctcactctcc aggagttttt taacttagtt ttataatgaa
     1321 tacg
//
LOCUS       DAZFKY010000401         1316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFKY010000401 DAZFKY010000000
VERSION     DAZFKY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(39..227)
                     /locus_tag="ACHKCL_005033"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHKCL_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          388 a          290 c          237 g          401 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcactgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg acctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat ttatga
//
LOCUS       DAZFKY010000402         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFKY010000402 DAZFKY010000000
VERSION     DAZFKY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            280..595
                     /locus_tag="ACHKCL_005034"
                     /pseudo
     CDS             280..595
                     /locus_tag="ACHKCL_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHKCL_005035"
                     /pseudo
     CDS             complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHKCL_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          383 a          305 c          279 g          345 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcaaga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcggtggt gcctgacggg cgggcttaaa acctaaagcc tcaggacctt
      961 tcgatttttc ataggcagcc aagtgctctt ttaatgcctg tgcaatgggc ggtaaaggtc
     1021 gggatgcccc ggatgcctgt ccacttgtcg gtggcggcgg tgccggacga gagggagtaa
     1081 atgaagtcac ctggctgctc acattaaaaa tcgttctcgc attaacattc gacgagcctg
     1141 gagaaaaagg ggaatgaact ttcaccggag taagacgcac ggcctgaggg gctacagaaa
     1201 atccagttcc ccccgcagag ctcactcgac ttgcgatacc tacaagctgc cgccctagtg
     1261 tagaagcagc gttactaatt ccattaagca taatttatgc ctctttatat ct
//
LOCUS       DAZFKY010000403         1306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFKY010000403 DAZFKY010000000
VERSION     DAZFKY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..138
                     /locus_tag="ACHKCL_005036"
                     /pseudo
     CDS             1..138
                     /locus_tag="ACHKCL_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154256.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
     gene            639..1248
                     /locus_tag="ACHKCL_005037"
                     /pseudo
     CDS             639..1248
                     /locus_tag="ACHKCL_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          381 a          243 c          268 g          414 t
ORIGIN      
        1 ttaacagcta tccgtatggc gtgttcggtt ttgcgaataa aaaatggtta ctgtagaagc
       61 ggatttccac cacatgtcaa ccatgaatgg tcagtgatgc gagacgactg ggatgatttg
      121 gtcgatgact tcttccggcg aatactgata gaaacattga aatattgtaa gagaaagcgt
      181 tcaaaattct gtggcacgtg aaaaattcta cagcgtcatc accttattca gtcgaccttc
      241 aaaataaacg tttaacagag acaatcacca tttggattgt ccgctgagga ggcgaacaat
      301 ttcctagaca gctttatcat gtcagcccac attgttggcg ctggcaatac taaggacaaa
      361 cgatggggaa gtagcttttt agcgttattt tcgggagtaa aaaacgtggt atctttccgt
      421 atgtcagttg tatcttgctg gtattcgtag tcgtgataag gcatacaacg atatttatct
      481 tgacgttagt gaagaataag ttgttttgtg atctatgtta atctgggatt acaatccggg
      541 cttgatacat cgcatcacga cttttattct cttcagaata gaatatgtta ctccccgctt
      601 tacaggctca ggatttgaat tacaaattat acttttaagt gaacaataac caagggtatt
      661 tcttatttcc tgaaaccagg cttggcccat ttcgggatga gtttgaaaaa actcttgacg
      721 catacgatag tgatgaaata aacgaaacaa attacgtcaa caaactcagg cgtcttgcct
      781 gacatgctcc tgacatcatt gatattcacg ctcatctggc atatgcattt gcgctcttta
      841 tgctgcaatt ctggcgaatg ttcatttaca gcgtcatcag gatgccgtta tactcattga
      901 taaaatacta acatataacc ctgagaataa tcagggaaca cgctggttac tgggggcgga
      961 attgttacgt gctggcaagc atggcagggc tctgtacgta ttgacaggac atgtcaatga
     1021 atttcccccc tactggtacg agcttgctct tttacatttt atgaatgagg catatattaa
     1081 tgctgcaacg gcatttcgat atggattttt aacaagtata tattgttgaa atgctttgtt
     1141 agaatcttta tccattccct agggctgtat agtatgattt ttcaggtatt tctgaatact
     1201 gccaaatatt attgcaaaat attctctaaa ttatgaatta aatatccata agcattattg
     1261 cctgggaact ggacatataa ttactcttct gtgctaaagg aacgtt
//
LOCUS       DAZFKY010000404         1289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFKY010000404 DAZFKY010000000
VERSION     DAZFKY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            57..>1289
                     /gene="pitB"
                     /locus_tag="ACHKCL_005038"
     CDS             57..>1289
                     /gene="pitB"
                     /locus_tag="ACHKCL_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5765804.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPGNTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGE"
BASE COUNT          284 a          313 c          350 g          342 t
ORIGIN      
        1 gttaaagtca ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc
       61 taaatttatt tgttggcctt gatatataca cagggctttt gttattactt gctctggcat
      121 ttgtgttgtt ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg
      181 ttatttatac tcgtgccatg cagccacagc ttgccgtggt gatggcggca ttttttaact
      241 tttttggcgt gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttgccaa
      301 ccgatttgtt gctgaatatg gggtcaaccc acggcctggc gatggtcttt tccatgctgc
      361 tggcggcgat tatctggaac ctgggaacgt ggttctttgg tttaccggcc tccagttcgc
      421 ataccttgat tggcgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat
      481 cggtgatgga tgcgttaaac ctgcgtgaag tgaccaaaat tttctcctcg ctgattgttt
      541 cccctatcgt cggcctggtc attgcgggag gcctgatatt cctgctgcga cgctactgga
      601 gcggaacgaa aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaaag
      661 gcaaacgtaa gccgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg
      721 cgttttcgca cggcgcgaac gacggacaaa aagggatcgg cctggtgatg ctggtactgg
      781 tggggattgc ccctgctggc ttcgtcgtca atatgaacgc gtccggctat gaaattaccc
      841 gtacccgcga tgccgttacc aacttcgaac actacctgca acagcatcct gaactgccgc
      901 agaagttgat tgcgatggaa cctccattgc ctgcggcatc tactgatggc gcgcaagtga
      961 cggagtttca ctgtcatccg ggaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc
     1021 tgccaggcaa tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc
     1081 gcattatgct gtgcatctct gatacttccg cgaagctggc gaaactgcca ggcgtcagta
     1141 aagaagacca gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg
     1201 ctccggtgtg gatcatcatg gcagtagcac tggcgctcgg cattggcacc atgattggct
     1261 ggcgtcgtgt tgcgatgacc atcggtgag
//
LOCUS       DAZFKY010000405         1282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFKY010000405 DAZFKY010000000
VERSION     DAZFKY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..146)
                     /locus_tag="ACHKCL_005039"
     CDS             complement(<1..146)
                     /locus_tag="ACHKCL_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5765805.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNG"
     gene            complement(190..1121)
                     /locus_tag="ACHKCL_005040"
                     /pseudo
     CDS             complement(190..1121)
                     /locus_tag="ACHKCL_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          381 a          278 c          198 g          425 t
ORIGIN      
        1 aaaccattaa ctttctttcc tgccaaaaca tcatccacaa aaaccagagc atagtcagaa
       61 ccacattctt gctttatttg ttgatttaga atgggtattg tttctctacc ataacttcca
      121 gcaccaaaaa taccatacaa attcattttc ccatctccaa agaaatatct tatcaaaacc
      181 aatgcaagat tatgaaagcc cgcacgctgc aatgcctgac gctaggaagt ttctgtccat
      241 gactgctttt cgcatcttac atgataatcc tgccttgaat acattatcat tgtcagatat
      301 taatagcgat gtgccgcatt ctagaaaatc attcgaatgc aactcgcctt cttatattat
      361 agtcgatttc attcattacc acatcaggct actgtgcaac ttattctcca tgcgccagtg
      421 atttcgattt actgtggcga acttctctgc ggttaaagca gcagatctaa tataatattt
      481 gatcgtcatt tcggattctt tcttttgctc tgctattatt gagagaaagt ggactgccat
      541 tcataaattc tgcagccctt tccattcaaa cgtgaaatca ataagctcat caggagcatc
      601 tcaaacaata tgaagacgga catcgtctct gccgtgcctc ttttcactaa ttgcgtaact
      661 gtcatgtttt ggattattta attctttcag cgtaaatatc tcctcaaaga ctctattaag
      721 ccgactctta tttcctttta cagcgaataa ataatcacat ctctgttttt atatcttctc
      781 tgcaatatct ttctggcaag ccatcgcatc agttatgatt atttttcctt taatacccat
      841 cattttagaa agttcatgaa taactgtgaa cgcattaggt ttctcatccg tcttgatcta
      901 tccgaggacc agactgtgca ttgctgaaac gcactaatga catgaaccac tcctctgcga
      961 cgactcttgt cataagtgtg ccgaagtgtt ttaccatcaa ttgcgatgac gtcgttatca
     1021 tctggaaaat ggcagacatg tattcagtta acaaagcact cgtgaaatat tgcaggactg
     1081 atgcaggata caactttggc tataatatcg taaacaggga taataaaaat ctattgtctc
     1141 aattatttta agttccattt tgttctcctt tttatataag gcacatttgt tattacataa
     1201 gcgatgaaaa aatgtcttaa gcaaaggaac gtctcaatcg tgacgtgaaa tgcaaattgg
     1261 tcagtacaaa actttatttc ta
//
LOCUS       DAZFKY010000406         1274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFKY010000406 DAZFKY010000000
VERSION     DAZFKY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1274
                     /locus_tag="ACHKCL_005041"
     CDS             <1..>1274
                     /locus_tag="ACHKCL_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5765806.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKD
                     RHTAVEVNYTDPQNGWQTSTELVEDPEAILRYG"
BASE COUNT          291 a          336 c          421 g          226 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgcctcga gaggcgaagg ataatctcaa atccacgcag
       61 atgatgagtg tgattgatgc gattggtgag ggaccgatag aaggcccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc cgtgatacac
      181 ggtgtgactg cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag
      241 tcctccggag ctgaaaccgg actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      361 gagaccacct caaagggtga ccgtaacccg acgtctgtcc gcctgctgat tcagcttgag
      421 cgtggtggta aatggatgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 ttcctggcgt cggtgattct ggataatctg ccgccccggc cctttaacat ccggatggtc
      541 agggagacgg cggacagcac cacggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agtttggcgg tcagcagatg acggtgaact accatatccg cggtcgcatt
      721 attcaggtgc cgtcaaacta tgacccggaa aaacgcacgt acagcggcat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac
      841 ccgcgctacg ggatgggaaa acgtctgggg gcggcggatg tggacaaatg ggcgctgtat
      901 gccattgcgc agtactgcga ccagacggtg ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcatacct gtcacaacag cgtaaggcgt gggacgtcct cagtgatttc
     1021 tgctcggcga tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac
     1081 cgcccgtcgg atgtggtgtg gccgtacacc aacagcgatg tggtggtgga tgataacggc
     1141 gtggggtttc gctacagctt cagcgccctg aaggaccgcc acacggcggt ggaggtgaat
     1201 tacaccgacc cgcagaacgg ctggcagacc tccacggaac tggtggaaga cccggaagcc
     1261 atactgcgct acgg
//
LOCUS       DAZFKY010000407         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFKY010000407 DAZFKY010000000
VERSION     DAZFKY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHKCL_005042"
     CDS             <1..75
                     /locus_tag="ACHKCL_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765807.1"
                     /translation="RISQDISTRDEGGGGTVVVVGRQG"
     gene            417..>1273
                     /locus_tag="ACHKCL_005043"
     CDS             417..>1273
                     /locus_tag="ACHKCL_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5765808.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYG"
BASE COUNT          332 a          304 c          383 g          254 t
ORIGIN      
        1 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaacggt cgtggttgtc
       61 gggcgacagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
      121 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
      181 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga atttttatgc
      241 cattaccggt tttaaccaac aggattatcg gtgggcatga aagaaaaccc cggtatctgc
      301 tgataccggg gttttcttta gcatggcaga aatgtgtttc atgcttttcg ggcgaaggat
      361 atccgacttc tgtacggaat ggcaagtggc ggttaattta ttcaggggaa ggctgtatgg
      421 gaaaaggtgg cggtaaggca cacacgcctc gtgaggcgaa ggataatctc aaatccacgc
      481 agatgatgag tgtgattgat gcgattggtg agggaccgat agaaggtccg gtgaagggac
      541 tgcagagtat tctggtgaac aaaaccccac tgacggacac ggacggcaat cccgtgatac
      601 acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg gaaggctttg
      661 agtcctccgg agctgaaacc ggactgggcg tggaagtgac gaaggcaaaa ccggtgacgc
      721 gcaccattac gtccgcgaac attgaccgcc tgcgggtcac cttcggggtg cagtcactgt
      781 tggagaccac ctcaaagggc gaccgtaatc actcttctgt ccgactgctg attcagttgc
      841 agcgtaacgg taactgggtg acggaaaagg atgtcaccat taacggcaag accacctcgc
      901 agtttctggc gtcggtgatt ctggataatc tgcctgagcg gccctttaac atccggatgg
      961 tccgggagac agcggacagc acctcggacc agctgcagaa taagacgctc tggtcgtcat
     1021 acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgattgtg gggctgcagg
     1081 tggatgcgga gcagtttggc ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca
     1141 tcatccaggt accgtcaaac tatgacccgg aaaaacgcac gtacagcggc atctgggacg
     1201 gcagcctgaa accggcatac agcaacaacc ctgcctggtg cctgtgggac atgctgaccc
     1261 acccgcgcta cgg
//
LOCUS       DAZFKY010000408         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFKY010000408 DAZFKY010000000
VERSION     DAZFKY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..599
                     /gene="nleB"
                     /locus_tag="ACHKCL_005044"
     CDS             <1..599
                     /gene="nleB"
                     /locus_tag="ACHKCL_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO5765809.1"
                     /translation="SDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIES
                     YAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVL
                     YAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGI
                     KRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            648..>1269
                     /gene="nleE"
                     /locus_tag="ACHKCL_005045"
     CDS             648..>1269
                     /gene="nleE"
                     /locus_tag="ACHKCL_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO5765810.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPG"
BASE COUNT          428 a          191 c          263 g          387 t
ORIGIN      
        1 tatctgatat ttaccttgaa aatatccata aacaagaatc ataccctgcc agtgagaggg
       61 ataattatct gttaggctta ttaagagaag agttaaaaaa tatcccagaa ggtaaggact
      121 ctttgattga gtcatatgca gaaaaaagag aacatacttg gtttgatttt ttcaggaatt
      181 tggccatgtt gaaggctgga agtttgttta cagagacggg aaaaactgga tgccataaca
      241 tatcgccctg tagcggatgt atatatcttg atgccgacat gattattacc gataaattag
      301 gagtcctgta tgctcctgat ggtatcgctg tgcatgtaga ttgtaatgat gagataaaaa
      361 gtcttgaaaa tggtgcgata gttgtcaatc gtagtaatca tccagcatta cttgcaggcc
      421 tcgatattat gaagagtaaa gttgacgctc atccatatta tgatggtcta ggaaagggta
      481 tcaagcggca ttttaactat tcatcgttac acgattataa tgctttttgt gattttattg
      541 aatttaagca tgaaaatatt ataccgaata ccagtatgta tacctgcagt tcatggtaag
      601 tcttccggtt gtggttgaaa aatatcgttc tttaggtata gattcagatg attaatcctg
      661 ttactaatac tcagggcgtg tcccctataa atactaaata tgctgaacat gtggtgaaaa
      721 atatttaccc ggaaattaaa catgattact ttaatgaatc acccaatata tatgataaga
      781 agtatatatc cggtataacc agaggagtag ctgaactaaa acaggaagaa tttgttaacg
      841 agaaagccag acggttttct tatatgaaga ctatgtattc tgtatgtcca gaagcgtttg
      901 aacctatttc cagaaatgaa gccagtacac cggaaggaag ctggctaaca gttatatccg
      961 gaaaacgccc aatggggcag ttttctgtag atagtttata caatcctgat ttacatgcat
     1021 tatgtgagct tccggacatt tgttgtaaga tcttccctaa agaaaataat gattttttat
     1081 acatagttgt tgtgtacaga aatgacagcc ctctaggaga acaacgggca aatagattta
     1141 tagaattata taatataaaa agagatatca tgcaggaatt aaattatgag ttaccagagt
     1201 taaaggcagt aaaatctgaa atgattatcg cacgtgaaat gggagaaatc tttagctaca
     1261 tgcctgggg
//
LOCUS       DAZFKY010000409         1264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFKY010000409 DAZFKY010000000
VERSION     DAZFKY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            95..442
                     /gene="tnpB"
                     /locus_tag="ACHKCL_005046"
     CDS             95..442
                     /gene="tnpB"
                     /locus_tag="ACHKCL_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5765811.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            492..>1264
                     /locus_tag="ACHKCL_005047"
     CDS             492..>1264
                     /locus_tag="ACHKCL_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO5765812.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPG"
BASE COUNT          270 a          365 c          377 g          252 t
ORIGIN      
        1 tcagctgtga ggtaacgttc cggcacggga cgctccgctt caatggcaat gtcagcgaaa
       61 agctcctgac tctgctgata caggaactga agcgatgatc ccgttacctt ccgggaccaa
      121 aatttggctg gttgccggta tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa
      181 agtacaaacg gcgctgaaag acgatcccat gtccggccat gttttcattt tccggggccg
      241 cagcggcagt caggttaaac tgctgtggtc caccggtgac ggactgtgcc tcctgaccaa
      301 acggctggag cgtgggcgct tcgcctggcc gtcagcccgt gatggcaaag tgttccttac
      361 gcaggcgcag ctggcgatgc tgctggaagg tatcgactgg cgacagccta agcggctgct
      421 gacctccctg accatgctgt aaatctcttt atcctggttg tcacagaata agcccggtaa
      481 aatacgggct tatgaacgac atctcttctg acgacatctt cctgctgaaa cagcgcctgg
      541 ccgaacagga agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa
      601 tagaccatct gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg
      661 aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga
      721 gcgatacgct gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc
      781 gcacccgtaa gccgttccct gaatcactac cccgtgacga aaagcgactg ttgcctgcgg
      841 cgccgtgctg cccgaactgc ggcggttcac tgagctatct gggcgaggat accgccgaac
      901 agctggagtt gatgcgtagc gccttccggg ttatccggac ggtacgggaa aaacatgcct
      961 gtactcagtg cgatgccatc gtgcaggcac ctgcaccttc gcggcccatc gagcggggta
     1021 tcgccggacc ggggctgctg gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc
     1081 tgtatcgcca gtcagaaata tacggccggc aaggtgtgga gctgaggcgt tcactgctgt
     1141 cgggctgggt ggatgcatgc tgccggctgc tgtctccgct ggaagaggcg cttcatggct
     1201 atgtcatgac tgacggcaaa ctccatgccg atgatacccc ggtccaggta ctgctgccgg
     1261 gtaa
//
LOCUS       DAZFKY010000410         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFKY010000410 DAZFKY010000000
VERSION     DAZFKY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..105
                     /locus_tag="ACHKCL_005048"
     CDS             <1..105
                     /locus_tag="ACHKCL_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765813.1"
                     /translation="LFWQGEGFGHELFPVTNKYNAWRWKNKKIIDISW"
     gene            274..>1263
                     /locus_tag="ACHKCL_005049"
     CDS             274..>1263
                     /locus_tag="ACHKCL_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765814.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQI"
BASE COUNT          305 a          313 c          417 g          228 t
ORIGIN      
        1 ttgttttggc aaggggaagg ttttggccat gaattattcc ccgtgacaaa caaatataat
       61 gcttggagat ggaaaaataa aaaaataata gatatttctt ggtaaggaaa atattccccg
      121 aaagaggcca catggggaat actgctcccc tcactgatac gggagtaccg ttacgacagc
      181 gcgggtaacg tatcaggcgt caccagccgg gaagattacg gacgggaaac acagcgggag
      241 taccggcttg accggaacgg ccaggtcacg gcggtgacag cctcaggcac cgggctgggc
      301 tatggcgaag gcgatgagtc ctatggctat gacagctgtg gctacctgaa ggcgcagtct
      361 gcgggcgggc accggataag tgaagagact gaccagtatg ccggaggcca ccggctgaaa
      421 caggccggaa acacgcagta tgactatgac gccgcaggcc ggatggtcag ccgcaccaga
      481 caccgtgacg gctatcgccc ggaaacagag cggttccggt gggacagccg ggaccagctg
      541 accgggtatt gcagcgcaca gggtgagcag tgggaatacc gccacgacgc cagcggcaga
      601 cgaacagaga aacgctgcga ccggaagaaa atccgcttca cgtacctgtg ggacggcgac
      661 agtattgcgg aaatccggga ataccgcgat gatgaactgt acagcgtacg gcacctggtg
      721 tttaacggct ttgagctgat aagccagcag tgcagccggg tacggcagcc gcacccgtcc
      781 gtggccccgc agtgggtgac acgaacgaat catgcggtga acgacctgac gggccgtccg
      841 ctgatgctct ttaacagtga aggtaaaacc gtctggcggc cgggacagac cagcctgtgg
      901 gggctggcac tcagcctgcc cgcagacaca gactacccgg acccgcgcgg ggagcgggac
      961 gcggaagccg accccggcct gctgtatgca ggacagtggc aggatgcaga atcggggttg
     1021 tgctataacc ggttccgata ttacgagccg gaaaccggga tgtacctggt gagtgatcca
     1081 ctggggttgc agggagggga gcagacttac cggtatgtgc cgaatccttg tgggtgggtt
     1141 gatccgctgg gattggctgc aagttctaaa atcagcagtt tgatggacta tattggcgat
     1201 ggtcgtcgtg ttagtgggca tacgggtttc ctggatgggg ttcgtttatc acgtagtcaa
     1261 ata
//
LOCUS       DAZFKY010000411         1257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFKY010000411 DAZFKY010000000
VERSION     DAZFKY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..100
                     /locus_tag="ACHKCL_005050"
     CDS             <1..100
                     /locus_tag="ACHKCL_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765815.1"
                     /translation="RIPTASQNRSPAPVFVVMSGEYLLDKKEEIIQ"
     gene            113..202
                     /locus_tag="ACHKCL_005051"
     CDS             113..202
                     /locus_tag="ACHKCL_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765816.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            222..>1257
                     /gene="nleL"
                     /locus_tag="ACHKCL_005052"
     CDS             222..>1257
                     /gene="nleL"
                     /locus_tag="ACHKCL_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO5765817.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSD"
BASE COUNT          382 a          200 c          260 g          415 t
ORIGIN      
        1 acgtattccg acagcttcac aaaaccggag tccggctccg gtttttgttg tcatgtccgg
       61 ggaatatttg ttagataaaa aagaggagat aattcaatag ggagttaaat taatgccgat
      121 aaatctgaca tcttatttgt gggtacaggg acagaaagtt gtcccggcag ttgtgttttc
      181 taattttact ttagtggact aagtaaaaag gagtgagata aatgctgccc actacaaata
      241 tctctgtaaa ttctggagta atatcttttg aaagtcctgt agattcacca tctaacgagg
      301 atgttgaagt tgccctcgaa aagtggtgtg ctgagggaga atttagcgaa aatcgtcatg
      361 aggttgcatc aaaaatactt gatgttataa gtactaatgg agagacttta tcaatcagtg
      421 agccaataac aacattacca gacttgcttc caggttctct gaaagaactg gttttgaatg
      481 gatgtacaga gcttaaatca ataaactgct taccccccaa cttatcttca ttaagtatgg
      541 ttggatgctc atcattagag gttataaatt gcagcatacc tgaaaatgtc attaatttat
      601 ctttatgcca ttgtagttct ttgaaacata tagaaggttc ctttcctgag gcactcagaa
      661 attccgtata tttaaatggc tgtaattcat taaatgaatc gcaatgtcaa ttccttgcat
      721 atgatgtcag tcaaggccgt gcctgcctga gcaaagctga gcttactgct gacttaattt
      781 ggttgtcagc taaccgaacg ggtgaagagt ctgctgaaga attgaattac tctggatgtg
      841 acttgtcagg tctaagtctt gtagggctga atttatcatc agtaaatttt tctggagcag
      901 tgcttgatga tacagatctc aggatgagtg atttgtctca ggctgtattg gaaaactgtt
      961 cttttaaaaa ctcgattttg aatgaatgta atttttgtta tgctaattta tctaattgta
     1021 ttattagggc tttgtttgaa aactctaatt tcagcaattc caatcttaaa aatgcatcat
     1081 ttaaaggatc ttcatatata caatatcctc caattttgaa cgaggctgat ttaacaggag
     1141 ctattataat tcctggaatg gttttaagtg gtgctatctt aggtgatgta aaggagctct
     1201 ttagtgaaaa aagtaatacc attaatctag gagggtgtta catagatcta tctgaca
//
LOCUS       DAZFKY010000412         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFKY010000412 DAZFKY010000000
VERSION     DAZFKY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(133..1233)
                     /gene="ompC"
                     /locus_tag="ACHKCL_005053"
     CDS             complement(133..1233)
                     /gene="ompC"
                     /locus_tag="ACHKCL_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5765818.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          357 a          278 c          267 g          354 t
ORIGIN      
        1 cgggggtatg gaataatatt aattgaggtg aatgaccaga aatatcaaaa attgctcatt
       61 tttacttaag gagtcataca gcagcactta aatcaaaaac cagcccagag aagggctggt
      121 caggagagga tgttagaact gataggtaat gcccgtagcg acgatgttat cggtgcgaat
      181 atctacggca cgggtaaagt cgctttcatc aatcaggttg attttataat caacataggt
      241 cgacatattt ttgttgaaga aatacgtcgc accaacgtcg atatactcaa tcatgtcctg
      301 atcgccgtag cggcccagat ctatgccttt cgatttcagg taagcgagtg aggggcgcaa
      361 gccgaagtcg aattgatatt gcgccaccac ttcaaaactt tgtgctttgt tagaaatata
      421 accatcgcca taggtggtca tgtttaatgt ttcagcgtaa ttagctgcaa agtagatgtt
      481 gttggcatca tattttatac ctgtagccca cagttctgca ttcttaccgg aggcattgag
      541 aggattagca gcggctcttt cctgcgcatt tgtccgatcg gatttggtat acaccgctcc
      601 gataccaaag ccatcataaa cataagttga ggagataccg aaaccgtcac cgttggcacg
      661 cgtgtagtca gcaccataaa gatgactgtt gtcagtacgt tcatttttgc cttgatattg
      721 cgcggcaaag ttcagccctt caattaagcc aaagaaatcg tagttgcgat aggttgctac
      781 accggtggta cggtatgtca tgaagacatc tttctgactc caggtatcgc caccaaattc
      841 tggcaacata tcagtccatg aaccaatgtc atagccgaca ccgtagttac ggccatagtc
      901 cagagagcca aattcactaa tttttaaacc agcataggcc agacgggtct tgttgtcgcg
      961 cgagccgttg gcttcatcgt tatagccttt aaaatcatat tcccagtgac caaaaccaat
     1021 catttgatca ttgatttgcg tttctccttt aaagccgaga cgggcataag ttttatcacc
     1081 atcgtcacgc ttatcatcag taaaataacg tagagcggta accttgccat aaaggtcgag
     1141 tttattacca tccttgttat agacttcggc agcgtgtgct acgttcgcag ctaacaaacc
     1201 agttacaacc accgcaacta tttttaattt catttaacag aatcctttta attatc
//
LOCUS       DAZFKY010000413         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFKY010000413 DAZFKY010000000
VERSION     DAZFKY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..81
                     /locus_tag="ACHKCL_005054"
     CDS             <1..81
                     /locus_tag="ACHKCL_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765819.1"
                     /translation="VRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            78..899
                     /locus_tag="ACHKCL_005055"
     CDS             78..899
                     /locus_tag="ACHKCL_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5765820.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1137..1202
                     /locus_tag="ACHKCL_005056"
                     /pseudo
     CDS             1137..1202
                     /locus_tag="ACHKCL_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          336 a          240 c          354 g          310 t
ORIGIN      
        1 gttcgtgctc agccagtatc tggtggacgt ctgggggtga agatttaccc cataatgctt
       61 gaagggcagg tcaaaaaatg aaactggaag atttaccgaa atactactcc ccaaaatccc
      121 ccggcctgac tgatgcatcg gcctcaacgt cgaaagatac gctgagtatc actgatgtga
      181 tggccgcgca gggcatgaca cagaattggg ctgagatggg gttttctgcg ttccttggga
      241 aaatgggcat tagtatgaat gacagagagc gggcaacaga attgctgaca gaatatgcac
      301 tcagtcggtg tgatcgcgtg gcggcgttaa gaaaactccc ggcagaaata aaaccggcag
      361 tgatgcgtat tatggcttcg tatgcgtttg aagattatgc ccgtagcgcg gcgagcaaaa
      421 aacagtgccc ctgttgtcac ggaaaaaaat ttattgaaag cgaggttttt acaaacaaga
      481 tccagtatcc ggatggtaag ccgccagtgt gggcaaagtg cacaaaaggc gtgtatccgt
      541 cttactggga ggaatggaaa aaagtcaggg aggtggtaaa agttgcctgt ccggagtgtg
      601 gagggaaggg ggaggtttcc accgcctgta aagattttcg tgggcgcggt gttgccattc
      661 atcgtgaaga gtcggtaaaa cgtggtatgc ctgttatcag agactgccag cgttgtggtg
      721 gtcgtggcta tgaaagatta ccttcaacgg aggcatttaa tgccatatgt aatgtaaccg
      781 atgccatatc tcttgataca tggaaaaaaa cagttaaacg tttttacgat acgctggtgg
      841 tgcagtttga tattgaagaa gcatgggcag aacaacaact gaaaaaggtg accagatagc
      901 tttgttgatt tttcccgaat ctgtggtaaa tttgccctaa cgatgggcgt tttatgcctg
      961 acgttagaag attttttaca cccgtcgcca ggcgggtttt tttatgactg aaatcacgcc
     1021 agtacagtaa acgcgctggt ggttgtgaat accggtcttt ctgcttgctg gctttttcga
     1081 caagagttat tggtgtgtca cgttaaccgg aaaaaggaaa gtttgagaaa cgcgatctgg
     1141 cacaggcggt gattaacgcc gcatacctgg tggcctgtgc agatggtgaa tgtgaggctt
     1201 cctagaaagc gaagatcgaa caggtactgc gtaatcagcc
//
LOCUS       DAZFKY010000414         1234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFKY010000414 DAZFKY010000000
VERSION     DAZFKY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1234
                     /locus_tag="ACHKCL_005057"
     rRNA            <1..>1234
                     /locus_tag="ACHKCL_005057"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          308 a          293 c          387 g          246 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggtcttgaca tccacagaac
      841 tttccagaga tggattggtg ccttcgggaa ctgtgagaca ggtgctgcat ggctgtcgtc
      901 agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt
      961 gccagcggtc cggccgggaa ctcaaaggag actgccagtg ataaactgga ggaaggtggg
     1021 gatgacgtca agtcatcatg gcccttacga ccagggctac acacgtgcta caatggcgca
     1081 tacaaagaga agcgacctcg cgagagcaag cggacctcat aaagtgcgtc gtagtccgga
     1141 ttggagtctg caactcgact ccatgaagtc ggaatcgcta gtaatcgtgg atcagaatgc
     1201 cacggtgaat acgttcccgg gccttgtaca cacc
//
LOCUS       DAZFKY010000415         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFKY010000415 DAZFKY010000000
VERSION     DAZFKY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            211..1170
                     /locus_tag="ACHKCL_005058"
     CDS             211..1170
                     /locus_tag="ACHKCL_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO5765821.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          453 a          234 c          220 g          326 t
ORIGIN      
        1 taaaataaaa ttataaaaaa cataaaacac cacgcaaaca cacttaataa aaacaccgtt
       61 acattaaaag ataataaaaa ccgcaataaa aaacgaataa atcaattgtc tcacgcaatt
      121 ataaaacatc atattgatta cgcaccttgc attacaaact catgtatgta aaatacgcgc
      181 accattcaaa aaaaaggaag acaataacat atgaaaaaaa gtgtcatcgc tggcgtcttt
      241 attgctctgt catttaccac gtgttcagct atcgcgaaca gccttgcatt atcattagca
      301 aatgatgatg cagggaagtt tcaaccaata cttaatgata tttatggcaa taaacatgaa
      361 aacagagatg attactcaca aggcttattt ctgggatata gccacgatat ctcagactcg
      421 agccaattat ctctccatat tgcgcaagat atttactctc catcaggcag taataaaaga
      481 cacaacacag ctgtaactgg agacagagct tttagtgcat acactcacac tggtattgaa
      541 tggaactccc ttgcgaatga ctggattcgc tatcgattag gtactgacat aggtgttgtt
      601 ggccccgacg caggcggtca gaaagtacaa aataaagctc atgagattat tggggcagaa
      661 aaatatcatg catgggatga tcaaatagag aatcgctacg gttatactgt aaaagggatg
      721 ctatccatga caccaagtat ggatatttta ggtgctaatg ttggattata ccctgaagtt
      781 tctgctgtta ctggaaactt atttcaatat gtagcatatg gcgcaaccat tgccattggt
      841 aatgataaaa ccttcaattc ggataatggc tttggtctgc tggctccccg tggtttaatg
      901 catatgtccg atacaagcgg attcaaatac aagatttttg caggtatgga aagacgagat
      961 gtcaatcgca actatactct cgaaggaaaa acaatacaga cgaaacaaac aacagtatcg
     1021 ctaaacaaaa ctgttgatga atatcaagtt ggcgcaacaa ttgggtatgc acctgtagcc
     1081 ttcacactag catttaataa agtaacatca gaattcaaga caggggatga ctattcattt
     1141 ataaatggag caatcacctt ctttttttaa ctgaattgaa ttcaatcaaa ataacataag
     1201 tccaacaaaa acataaagtg cgaaatgaat gcc
//
LOCUS       DAZFKY010000416         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFKY010000416 DAZFKY010000000
VERSION     DAZFKY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1222)
                     /gene="espX6"
                     /locus_tag="ACHKCL_005059"
     CDS             complement(<1..>1222)
                     /gene="espX6"
                     /locus_tag="ACHKCL_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO5765822.1"
                     /translation="IDKNNLKESIFSVLHNDPQQVDKDQHLPCEKFLKHACKSSNSFE
                     VKLDATHQYQHLNNFMISFDPVENQLTIRDNNNKTETFSFTNLQWENLLQYYKENHQQ
                     PNIAGSRNLTDNIDKIKNTISTSEIIECASPEIRSSVLNDLYSIANFLPDNNLTPNES
                     WKRFCETCERFYVAQKSITGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPED
                     ATGQRCIEGLNLAEMDLTDIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLR
                     NAEFENASLENNDFRKVRHLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHI
                     DFTTVILYETSKIPGIPGTPQIPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYT
                     NFSNANLTNCKISNA"
BASE COUNT          341 a          211 c          240 g          430 t
ORIGIN      
        1 tgcattagag attttacaat ttgttaaatt agcgtttgaa aagttggtat acatgcagag
       61 aataccagta agattatatt ctgaaagatc ttttcccgat agatcggaat aatttagtat
      121 tgcgccagta agaattactt taggtgttcc cggtatttga ggtgttccag gtattccagg
      181 tattttagat gtttcatata gaatcacggt tgtgaaatct atgtgttcaa gatacgcgtt
      241 actaaggtca cttccagtaa aggttacgcc agtgagaact tttccgttga agttactgtt
      301 tcgtagattt gcgtttttga aatttacata agtcaagtgg cgaactttac gaaaatcatt
      361 attttctaat gaggcatttt cgaattctgc attacgcaga tcacaatcgg taaaatccac
      421 gccttcacag atcgtaccgg agaacttggc atttctaaga atgctgccat taaaattgac
      481 attccttagc gccattttcg acaagtctat gtcggttaaa tccatctctg ctaaattcaa
      541 cccttcgata caccgttgac ccgttgcatc ttcaggaata gcagtgctaa tagtattgat
      601 gacaacatca cctatcttga aggtcattgt aattcctgca tcagagatag agagttttcg
      661 cgtaagacgt tcactcttat ctccagtgat actcttctga gcaacgtaaa agcgctcgca
      721 tgtctcacaa aatcttttcc agctctcatt tggggtcaga ttattgtccg ggaggaaatt
      781 agcaatgcta taaagatcgt tcaggacgct acttcttatt tcaggagagg cgcattcaat
      841 aatttcagag gtggatattg tatttttaat tttatctata ttatccgtga gatttcgtga
      901 tcctgctata tttggctgct ggtggttttc tttgtagtat tgcagcaaat tttcccattg
      961 taagtttgta aacgagaaag tttcagtctt gttattgtta tcccgtatgg ttaattgatt
     1021 ttctactggg tcaaaagaaa tcatgaagtt attcaggtgt tgatattgat gagtggcatc
     1081 taatttcact tcaaatgaat ttgaactttt gcaggcatgt tttaaaaact tttcacaggg
     1141 aaggtgttga tctttatcta cttgttgtgg gtcattgtgc aaaacagaga aaatactctc
     1201 tttcaaattg ttcttgtcaa ta
//
LOCUS       DAZFKY010000417         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFKY010000417 DAZFKY010000000
VERSION     DAZFKY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..544
                     /gene="lpfA1"
                     /locus_tag="ACHKCL_005060"
     CDS             20..544
                     /gene="lpfA1"
                     /locus_tag="ACHKCL_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO5765823.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            609..>1222
                     /gene="lpfB"
                     /locus_tag="ACHKCL_005061"
     CDS             609..>1222
                     /gene="lpfB"
                     /locus_tag="ACHKCL_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO5765824.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSV"
BASE COUNT          335 a          258 c          293 g          336 t
ORIGIN      
        1 tttttaaatg gagtttttca tgaaaaaggt cgtttttgcc ttaaccgcac tggcgttaac
       61 ttctggcact gtattcgcag cagagtctgg tgacggcacg gttaaattta caggcgagat
      121 cgtggattca ccttgcgtac tgtccgttga ctctcagaac caggaagttg tgttgggtca
      181 ggtacaaaaa tctgtatttg ctgcggttgg tgacaaatcc ccggcaaaac ctttcgaaat
      241 caaactggaa gattgcgata ccaccacgat gaaaaaagcc aatgtgagct ttagtggtgt
      301 tggtgatgca gataaatctg atctgatttc tgttagcact gaagcgggcg cggccaaagg
      361 tgttggtatc ggtatttatg ataacagcaa taccctggtc gctcttaacg gtggtaaagc
      421 aagtgttgat ctgagtaaag gccagacggt tctctacttt accgctaact acgtttctac
      481 tctggcaacg gtaacgaccg gttacggcaa tgcgcaggtc gatttcaacc tgtcttacga
      541 gtaatcgtcc gttttacaaa tggcggcgta attgccgcca ttagtcgtca aagttcatgg
      601 atcgaatgat gaaaagtaaa tttgttgcgt tggctctctc tctgttcctt tcacagtcag
      661 ttctggcggg tggcgtcgga cttagctcta ctcgcgtaat ttatgatggc agtaagaaag
      721 aagcctcgct gaccgtacag aataaaagta agacggaaga gtttcttatc caatcctggg
      781 tggatgatgc cgcgggcagc aaaaaaacac cgtttattat tacgccccca ctattcaagc
      841 ttgatccaga aaagaacaat attttgcgta ttgtgaatat tacgcccggc ctgcctcagg
      901 atcgcgaatc ggtctactgg gttaatgtga aagccattcc atctaaaagt gacgacagcg
      961 aaaacaaaaa tgttttgcag attgccgttc gcacccgaat caaacttttt tatcgcccgg
     1021 caggtttaaa gggtgatgta aaaaccgcgc caaatgaact gcgatttacg cgtaacggta
     1081 atcagctccg cgttgataat ccgacagttt ttaatattac ctttaatcaa ttttttgcga
     1141 atgacaaaga aattgaaaaa gcaggcatgg tacccgctaa aggggcatta aatatcacat
     1201 tacccgccgg ggtaggtagc gt
//
LOCUS       DAZFKY010000418         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFKY010000418 DAZFKY010000000
VERSION     DAZFKY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..474)
                     /gene="ybfC"
                     /locus_tag="ACHKCL_005062"
     CDS             complement(<1..474)
                     /gene="ybfC"
                     /locus_tag="ACHKCL_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO5765825.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLP"
     gene            complement(499..>1220)
                     /locus_tag="ACHKCL_005063"
     CDS             complement(499..>1220)
                     /locus_tag="ACHKCL_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765826.1"
                     /translation="EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAR
                     PNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPN
                     LTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAG
                     IDDLGEILAKMKQRTSRGIRK"
BASE COUNT          307 a          283 c          240 g          390 t
ORIGIN      
        1 cgggagatgt gctgtcattc gagcataagt tggcttctca tctttaaaat atttaaccat
       61 gattgaatta tcaaggactg ccaacaactg agctggatca ctgctctttt tttgatagat
      121 acccgctctt atataacttc taaaccgatc gccgagaaac gccgtttttt ctgctgtatt
      181 tatcaactcc tgcaacgttc ctttgatggc atgattttca ttaaggcgta gtgacccgac
      241 acgaattaag agtctcgccc cgcttggtgt gaatttatta tttttacatg tacccagttc
      301 tgtttgccaa taatactcac cgatgtcctc attctgatac catctccaca tggctttatt
      361 atttaagaca ggaaacgcgg aaaaaatagt acccttagcg aaaatagtaa aacaatcatg
      421 atctttagct tgtatttcgc aatcagctat aacaccaaaa ctaacaaata tcatcagcaa
      481 aaaaaaacaa aactcttttc attttcttat tcctctcgat gttctctgtt tcattttagc
      541 taaaatttca cccaaatcat ctatccctgc tgttgctggc ccatcgggtt tattaatgtc
      601 agggaataaa tagggttctg cacaagtata tcctggtggt aattgagttg ttggccaatt
      661 tgtctgtggc aggtaatcac ttgctgtctt tgtatccatc cctggatact ggggatcatc
      721 ctgtggcgta caagtcagat ttggttttac ccacttcctg caatacagtg tgtagtcgta
      781 agggcttaat gctctgaaga atgttttgaa aacattaggc gcactactgg cctggtcaat
      841 aggatatggc agtcttaatt gtttcgctat gtctggaaat ttacttggat cgggcgtagg
      901 cggtttgttg ggtctcgctc catatccgcc attatttaag tgtccatatt ttgatgccag
      961 tcccattgaa tctatatact gaaccggatt caacggatac tgataaaaat tccatccccc
     1021 cttcagtcca atcggatcct gagtgatata cctcccctgc agcgggtcat aatagcggtg
     1081 gcggttgtaa tacaggccgg actcctcatc atactgctgc cccggcaggc ggataagctg
     1141 ctgcagctga tgcgggttct cttcattcag caggttgccc cactcatcgt attctgcgca
     1201 ccattctgtt gccccttcct
//
LOCUS       DAZFKY010000419         1217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFKY010000419 DAZFKY010000000
VERSION     DAZFKY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHKCL_005064"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHKCL_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5765827.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVR"
     gene            704..>1217
                     /locus_tag="ACHKCL_005065"
     CDS             704..>1217
                     /locus_tag="ACHKCL_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5765828.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLW"
BASE COUNT          367 a          252 c          227 g          371 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcacgtaa gaggctaacc tagatttgcc gttcatagat tgagcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctatctgccg ttggtgttca
      241 tgcccgaggc agatagcctc aagcacccgc agctattcta cttaacttcc cattacctcg
      301 ccaatatgaa atcggtcaga aagacagacg tcccataagg acaacaacgg aaaaagagat
      361 tttgcttgat gctatttacc tctgcaccat gaagttgcgc caaaatccca gtggtgataa
      421 caggaatttg gttgttggtg attggggaaa gaagtacgtt agactgtggg ttagttcatt
      481 gacatgatag ttacctgcct tcaatttcct aagcaagcga tgtaaacgat aatcaatatc
      541 aaatgtattg acaggtaaaa taataacgat gtacctttgc tgataacata tatcagtctt
      601 aaatcataat ggaggtagat aaaataacta aacacacaca acaaattttt tatcagtgac
      661 atagcatatc ttaagcatcc tttaatgctc atggggagaa cgcatgacta aacatatgga
      721 cattcattgt caaacacgca tatttaactc aattaaccat gacaccaaat ataaaatatg
      781 tatttttact ccaaaaaaca ttaaaaaaaa taatgcaact ctttatatac ttgatgggaa
      841 tagtgccaac aattacattt ctgatattct gcctgttatt gatgcactac ccaatccacc
      901 agtgttagtt acacttggtt atgaatcttg gaataacctt agcattcatc gccgtgctta
      961 cgattatact cctgatggtg aaaatgctat tgttgataac tctaaaccag catggatcta
     1021 ctttactggt ggaggaagcc agtctttccg agaattatta ctaactcaga tcatgccatg
     1081 ggttagtact attgctccga acagttccag aatcggtata tggggacact cgctaggtgc
     1141 tatttttgtg cttgattgct taaaaaataa ttcatgcttt aactattatt atatatctgc
     1201 accatcactt ctatggc
//
LOCUS       DAZFKY010000420         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFKY010000420 DAZFKY010000000
VERSION     DAZFKY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1150
                     /gene="ygeH"
                     /locus_tag="ACHKCL_005066"
     CDS             <1..1150
                     /gene="ygeH"
                     /locus_tag="ACHKCL_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO5765829.1"
                     /translation="SLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNTSDYSI
                     AIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLRRFN
                     PDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDELHNTSQFIIENILQTVHN
                     PQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKSDIQTLK
                     TECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSG
                     QAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRKAVV
                     IKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLKQLTRICMR"
BASE COUNT          447 a          212 c          198 g          356 t
ORIGIN      
        1 ttctttgcgc tgcatttttg aaaaaattgg ctatgatcgt tgcatagaaa caatctaccg
       61 gaaaggttat cgtttcagtg ggcaggtttt caaaactaaa ataaatgaag ataacacttc
      121 agactattct atcgctatat tccctttcac tacttcattg aaaacactgg atccattaat
      181 acttaatcag gaattagtac aaatcatttc aaataaaaaa atcgatggcc tctataccta
      241 tccgatggct gcgacaaatt tttgtaatga tcacatatcg caaaattcat ttttgagaag
      301 attcaatcca gattatttcg ttacaggaag aataaaccag aataatgcag tgaacacttt
      361 atacattgag ttgaccgacg ctaaaaacct tttcctcatc gccagtaatc atctacctgt
      421 tgatgaacta cataatacat cacaatttat tatagaaaat atccttcaaa cagtacataa
      481 tccacaacga tctgtaagat taactaagca ggaccaagga tataagaatc attatttatc
      541 agatgaaatg ttagccggaa agaaagaact ttacgagttc acccctgaaa gcatttacag
      601 ggccatgact atatttgatg gattacaaaa taaaagtgat atacagacgc taaaaacaga
      661 atgttattgc cttctagcgg aatgccatat gtctttggca cttcatggaa aaagtgaact
      721 tgaacttgct gctcaaaaag cattagagct tttagattat gtatcagaca taaccactgt
      781 cgatggaaaa attttagcta ttatgggact gataactggt ctgtctggac aagcaaaagt
      841 atctcatatc ttatttgaac aggctaagat acactcaact gatatagcct ctctctacta
      901 ctatagggca cttgtcaact ttcataatga aaaaattgaa gaggcaagga tttgtataga
      961 caaatcacta caactcgaac ccagaagacg aaaagcagtt gtgataaaag aatgtgtaga
     1021 tatgtatgtg cctaacccgc tcaaaaaaaa catgaaactc tactataaag aaactgagag
     1081 tggaagccat cgagttataa ttgacaacat tttgaaatta aagcagctga cgagaatttg
     1141 tatgcgataa atttttattg acacaatttt attatttaga tgtatgcaac ttgttattta
     1201 aaaaacaaag atc
//
LOCUS       DAZFKY010000421         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFKY010000421 DAZFKY010000000
VERSION     DAZFKY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1196
                     /locus_tag="ACHKCL_005067"
                     /pseudo
     CDS             1..1196
                     /locus_tag="ACHKCL_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          427 a          242 c          224 g          303 t
ORIGIN      
        1 gacagagaaa ttatatcaac ttttcacgca gaaaaagtca ttaatgatct gttaaaaaac
       61 gattataaaa tttccctttc tccagataca acttaccgag agctgaataa agctgcacag
      121 cgtagcatta cagcgccaga caggatagga gaagggaaaa tatgggttta tcaacgagat
      181 acaatggttg aaagaggtga taacagcggt gttcatcagt atggtccagc tgaacatttc
      241 actcacatta tatctgacaa accgtcccca aaagataagt atgttgcata tgctattaac
      301 attcctgact atgagctagc agccgatgta tataatatta acgtgacgtc accttccgga
      361 cagcaagaaa catttaaaat attaatcaat ccagaacatc tacggcaaac acttgagcgt
      421 aaatccctta ctgctgttca gaaatcacaa tgtgaaatca tcacccccaa aaaacctggc
      481 gaagcgattc ttcatgcttt taatgccacc taccagcaaa tcagggaaaa tatgtctgag
      541 tttgcacgtt gccattatgg gtatatacaa atccctccag tgacaacttt ccgcgccgac
      601 ggaccagaaa ctcccgaaga agaaaaaggt tactggtttc acgcttatca acccgaagat
      661 ctttgtacca tccataatcc aatgggagat ttgcaggatt ttatcgcatt ggttaaagat
      721 gctaaaaaat ttggtatcga tatcattcct gattatacct ttaactttat gggaatcggg
      781 ggtagtggta aaaatgacct ggattatccc tctgctgata tacgagcgaa gatcagtaaa
      841 gatatagaag gtggtatccc tggctattgg caaggtcagg ttttgattcc atttactata
      901 gatccagtaa caaaagaacg taaacaaatc catccagaag atatacatct cacagcaaaa
      961 gacttcgaag caagtaaaga taatatttct aaggatgaat gggaaaacct ccatgcatta
     1021 aaagaaaagc gtttaaatgg aatgcctaaa acccccccaa aagtgactag gttattatgt
     1081 tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttacgatg
     1141 cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaa
//
LOCUS       DAZFKY010000422         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFKY010000422 DAZFKY010000000
VERSION     DAZFKY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..253
                     /locus_tag="ACHKCL_005068"
     CDS             <1..253
                     /locus_tag="ACHKCL_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5765830.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHKCL_005069"
     CDS             268..801
                     /locus_tag="ACHKCL_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765831.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHKCL_005070"
     CDS             798..>1180
                     /locus_tag="ACHKCL_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5765832.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZFKY010000423         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFKY010000423 DAZFKY010000000
VERSION     DAZFKY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1176
                     /locus_tag="ACHKCL_005071"
     CDS             <1..>1176
                     /locus_tag="ACHKCL_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765833.1"
                     /translation="VKAMFRGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMAS
                     QAQAFEEKLTSLSAVLQKHVTVDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAV
                     NDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAV
                     ANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIP
                     QPADGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQD
                     GRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSM
                     TGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMT
                     "
BASE COUNT          259 a          275 c          397 g          245 t
ORIGIN      
        1 gtgaaagcga tgttcagggg ggatactgag aaaatgacgg aacgggaact gtccattatt
       61 cgtgcactgg gagaagaatt ttccacagtg ctggcggatt tacagcgcac atttgagggg
      121 aagatggcct cgcaggcaca agcgtttgaa gagaaactga cttccctgtc ggcggtatta
      181 cagaagcatg tgacggtgga tgaggtgcgt ccggttctgc aggcgatggt ggatgacgct
      241 gtgggggcca ttccggtacc gcgtgatggt cgtgattatg atccggatgt actgcagcag
      301 gcggtgaatg atgcggtcgc aaatattccg cagccggcgg acggtaaaag tctcaccccg
      361 gatgatgtgc gtccgatgct tgaacagatg gtgaaggagg ctgtaagcca tatccctgtt
      421 ccgcgtgatg gtcgtgacta cgatccggaa gtactgcaga aggcggtgaa tgatgcggtc
      481 gcaaatattc cgcagccggc ggacggtaaa agtctcaccc cggatgatgt gcgtccgatg
      541 cttgaacaga tggtgaagga ggcggtaagc catatccctg ttccgcgcga cggtcgtgac
      601 tatgatccgg aagtactgca gaaggcggtg aatgatgcgg tcgcaaatat tccgcagccg
      661 gcagacggta aaagtctcac cccggatgat gtgcgtccga tgcttgaaca gatggtgaag
      721 gaggcggtaa gccatattcc tgttccgcgt gatggtcgtg actacgatcc ggatgttctg
      781 cagaaggcgg ttctggatgc ggtgagtgcc ctgccggctc cgcaggacgg gcgtgatgcc
      841 acggcactgg aaatactccc cgccattgac gatcaaaaat cctttccccg gggcacgtat
      901 gccacacacc agggcggact ctggcgggcg tatgaaaaaa cgcacgggat gcggggatgg
      961 gaatgcctgg ttgatggggt ggcggatatt gacgtcagca tgacgggtga gcggttgttc
     1021 tctgtggtgg tccggcagag cagtggccag cgtacggaaa aaacattttc cctgccggtg
     1081 atgctctacc gcggtgtgtt cagagccggt gaaacctacc accccggcga tacggtgacg
     1141 tgggggggct cgctgtggca ctgcaacagt atgacc
//
LOCUS       DAZFKY010000424         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFKY010000424 DAZFKY010000000
VERSION     DAZFKY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1175
                     /locus_tag="ACHKCL_005072"
     CDS             <1..>1175
                     /locus_tag="ACHKCL_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765834.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKETIKALQDAGYLK"
BASE COUNT          321 a          247 c          378 g          229 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaagaaacg
     1141 ataaaggctt tacaagatgc tggctatctc aagta
//
LOCUS       DAZFKY010000425         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFKY010000425 DAZFKY010000000
VERSION     DAZFKY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            138..245
                     /gene="yniD"
                     /locus_tag="ACHKCL_005073"
     CDS             138..245
                     /gene="yniD"
                     /locus_tag="ACHKCL_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5765835.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(298..1056)
                     /locus_tag="ACHKCL_005074"
     CDS             complement(298..1056)
                     /locus_tag="ACHKCL_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5765836.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          325 a          280 c          273 g          281 t
ORIGIN      
        1 ttattttttc ataaaacgca gaaacagtga tcaggtgtac aacgccaccc gacggctgta
       61 ataacaaaaa atcccttgat gcctgtccct tttgttacac tccgttatca cgcacaagag
      121 atatgcagga cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg
      181 tggtgctact ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg
      241 gctgagcagt gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta
      301 catgctgtag cccagtgata aggttgtccg acggtcggta tgttccggtg cagactctgg
      361 aggttcagag ttccatgtga cgttgtaagc caccttcagt ccaaaatgct cattaatagc
      421 aacatttaat gcgctttcgg agttcagcgt tgtgtcttcc gcgccaaaga cggaaacacc
      481 ctgcgtaaat ttagcgttgt cagtaagctg ccatgcataa gcaccggagg catagcccag
      541 cggctgagtt tcactggcat tgtcggtata tttgtcgtaa cgcacacctg gaccgaattc
      601 aaagcggaaa ctgtgtaccg ggccattcaa aaactgacga ccataacccg cggtcaacac
      661 atcgcgctct cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata
      721 gtcataatca gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg
      781 ctcatcatta gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc
      841 ataccaggtc atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc
      901 aagataccct gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat
      961 gacagtaaaa acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa
     1021 cataacgatg gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa
     1081 aaagagacca tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa
     1141 ggtaacgaat tataaaaag
//
LOCUS       DAZFKY010000426         1156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFKY010000426 DAZFKY010000000
VERSION     DAZFKY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..>1156
                     /gene="espG"
                     /locus_tag="ACHKCL_005075"
     CDS             38..>1156
                     /gene="espG"
                     /locus_tag="ACHKCL_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO5765837.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAA"
BASE COUNT          364 a          246 c          230 g          316 t
ORIGIN      
        1 caaattgttc attacaaacc agataggaga atctctcatg ataaatggac ttaataatga
       61 ctccgcatct ttagttttag atgctgcaat gaaagttaat tctgggttta aaaaaagctg
      121 ggatgagatg tcatgcgctg aaaagttatt taaagtactt agttttggtt tatggaatcc
      181 aacgtacagt cgtagtgaaa gacaatcatt tcaagagttg ttaaccgttt tagagcctgt
      241 atatccactt cccaatgaat taggcagagt atctgctcgt ttttcagatg gttcatcctt
      301 aagaatttcc gtcactaaca gcgaacttgt tgaagccgag attcgcacag caaataatga
      361 aaagattact gtgctcctgg agtcaaacga acaaaatagg ttattacaat ctttacccat
      421 cgatcgccac atgccataca ttcaggttca tcgtgcctta tctgagatgg acctgactga
      481 tactacctca atgcgcaatc tacttggttt tacgtcaaaa ctatcaacaa ccttgattcc
      541 tcataatgct caaacagatc cgctttccgg gcctacacca ttcagctcta tctttatgga
      601 tacatgtcga ggactcggca atgcaaagct ttcactcaat ggtgttgata tacctgcaaa
      661 tgcacaaaaa ttgcttcgcg atgcactagg acttaaagac acacattcat caccaacccg
      721 gaatgttata gatcatggta tttctcgcca tgatgcagag caaatagcaa gagaaagcag
      781 cggcagtgat aaacagaaag ctgaagttgt ggaattttta tgccatccag aagcagcaac
      841 ggccatatgc tcggctttct atcaatcttt caatgtgcca gccttaacgt tgacacatga
      901 aaggatctct aaagccagtg aatacaatgc ggaaagatca ttagatacac ctaacgcttg
      961 cattaacatc agtatctctc aatcatcaga tggaaacatt tatgttacca gccatactgg
     1021 ggttctgata atggcgccag aagaccgccc caacgagatg ggcatgttga cgaacaggac
     1081 ttcttatgaa gtgccgcaag gtgtgaaatg tataatcgat gaaatggtaa gtgcgctaca
     1141 accaaggtat gccgca
//
LOCUS       DAZFKY010000427         1156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFKY010000427 DAZFKY010000000
VERSION     DAZFKY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..563
                     /locus_tag="ACHKCL_005076"
     CDS             <1..563
                     /locus_tag="ACHKCL_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5765838.1"
                     /translation="VELGAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAE
                     LVSQTYKLPHTQFEQIANLYEMQFDDGRTCAFHTDAQKAEQWLQACDGNRVQEYVKLE
                     RLQNALSGNSPVTPDGWISCSERMPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDAN
                     DGWIIPGASWKPSHWMPLPEPPQEVN"
     gene            563..958
                     /locus_tag="ACHKCL_005077"
     CDS             563..958
                     /locus_tag="ACHKCL_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765839.1"
                     /translation="MENEGDNIITLVQPKRDEEKLLNITVTGRKNYTQQSCKHRAIEV
                     HEQDHVILCLQCGCVVDPFQYVLRCANDGEAVVREIRQLHNRHDQLRESVASLEREEK
                     NTKARLRAARTAILYAENDLKNIEQKVNQ"
BASE COUNT          334 a          239 c          335 g          248 t
ORIGIN      
        1 gagttgagct gggagcagca gagaacaacc ttattgatag tgaatgccat gttgctgaac
       61 tggaagaagc gctacgcgat aagcaggcgt tacttgaagc atcagagaag cgcatagcag
      121 aactggaagc cgaactcgta agccaaactt acaagttgcc acacacgcag tttgaacaga
      181 ttgctaacct ctacgaaatg caatttgatg acggtcgcac ttgtgccttt cacactgatg
      241 cgcaaaaggc tgagcaatgg cttcaggcgt gcgacggaaa cagggttcag gaatacgtta
      301 agctggaacg gttgcagaat gcgctgtctg gcaactctcc ggtaactccg gatggttgga
      361 taagttgtag tgagcgaatg ccggatacca aaacagccgt tcttgttgcc gtggagtttg
      421 acaggaaagg tgactggcga atgaagtggg cgacttacat cccggggcat cctgacgcta
      481 atgatgggtg gataattcct ggtgcgtcgt ggaaaccgtc acactggatg ccgctaccag
      541 aaccgccgca ggaggtgaac tgatggaaaa tgaaggcgat aatatcatca cactggtgca
      601 gccaaagcgc gatgaagaga agctgctgaa cattacagta actggcagaa aaaactacac
      661 acaacagagc tgtaagcatc gggccattga ggttcatgaa caggatcacg ttatcctgtg
      721 cttgcagtgc ggatgtgttg tggacccttt tcagtatgtt ctgcggtgtg cgaacgatgg
      781 tgaggctgtg gtgagagaga ttagacagct tcataacaga cacgatcagc tccgagaatc
      841 tgttgccagc ctcgagcgtg aagaaaagaa caccaaagca cggttgcggg cagcaagaac
      901 tgcaatactg tatgcggaaa atgaccttaa aaatattgag cagaaggtga atcaatgacc
      961 tggcctgaag cattcgcaaa tgtaggaaca gtaatggcgg tggtgctggt tgtgtattcg
     1021 atttgtcgct ggagataaaa acggtttgcg ggaaaaggag agttaagtag aattgctgcg
     1081 ggtgcttgag gctatctgcc tcgggcatga acaccaacgg cagatagaga aaagccccag
     1141 ttaacattac gcgtcc
//
LOCUS       DAZFKY010000428         1150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFKY010000428 DAZFKY010000000
VERSION     DAZFKY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHKCL_005078"
     CDS             complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHKCL_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5765840.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          394 a          257 c          221 g          278 t
ORIGIN      
        1 taaaaagtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
       61 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca
      121 ggtttttata tagcgtctgg agacatggat atttatgcaa tgtaatcaac gccagttgca
      181 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      241 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
      301 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
      361 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac
      421 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
      481 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
      541 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
      601 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      661 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
      721 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
      781 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      841 aattatgact gcggttgctg aataatccaa caataagcgc aatgcccgaa cagattaata
      901 atgcacctac tactgtgctt aaaatatcgc cagaaacgaa cgggaaactg atacacagca
      961 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgc tttttcagca
     1021 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     1081 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     1141 cgatttaata
//
LOCUS       DAZFKY010000429         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFKY010000429 DAZFKY010000000
VERSION     DAZFKY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..441
                     /locus_tag="ACHKCL_005079"
     CDS             <1..441
                     /locus_tag="ACHKCL_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5765841.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHKCL_005080"
     CDS             597..779
                     /locus_tag="ACHKCL_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765842.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHKCL_005081"
     CDS             792..923
                     /locus_tag="ACHKCL_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765843.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1148
                     /locus_tag="ACHKCL_005082"
     CDS             931..>1148
                     /locus_tag="ACHKCL_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO5765844.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELA"
BASE COUNT          288 a          237 c          341 g          282 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagc
//
LOCUS       DAZFKY010000430         1139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFKY010000430 DAZFKY010000000
VERSION     DAZFKY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(40..195)
                     /locus_tag="ACHKCL_005083"
                     /pseudo
     CDS             complement(40..195)
                     /locus_tag="ACHKCL_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            345..1013
                     /locus_tag="ACHKCL_005084"
     CDS             345..1013
                     /locus_tag="ACHKCL_005084"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5765845.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          330 a          250 c          221 g          338 t
ORIGIN      
        1 caccactttg tctaattcgt cagattctga ccagacggtt caggctgtac gcttacgagc
       61 tactgatgta tcaacacgat tcaacaatac ccgatattta ttccatgcct ccagcaacga
      121 tctttcttcc tccgttgcga tttccagatc tacagcatcc tgcagtggcg caatatactc
      181 actgaattcc tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgctg
      241 tatcgcagca taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt
      301 aattcatcgc cataattgat ttaattcaca aataaaacta taacatggtg aaattaatga
      361 aaaaaaacac agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact
      421 ggtgggtttt gggagtatca aatcgtcttg catggggatg tcctacaaag gaacaccttc
      481 ttccacactt tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt
      541 tttaccttac tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta
      601 gcctgaacgc ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg
      661 atgtttttga accttatccc gcggcgttac atggtcaatt tgattccatt tccatgtttt
      721 accttcttca ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg
      781 cgcaggcctt aactgacgat ggaactctat acggagccac aattcttggc gatggagttg
      841 tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa
      901 acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta
      961 aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa
     1021 ccgcagcacg ttcttgcata cgccgtgtct gcggtttaat ttttactctc aaatattgta
     1081 gttaagtaca ataatgattc ctgtggctat atagatgttt ttgtaattag tccttaaga
//
LOCUS       DAZFKY010000431         1131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFKY010000431 DAZFKY010000000
VERSION     DAZFKY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            208..>1131
                     /locus_tag="ACHKCL_005085"
     CDS             208..>1131
                     /locus_tag="ACHKCL_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765846.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLT"
BASE COUNT          352 a          229 c          211 g          339 t
ORIGIN      
        1 gaccgtaagc ccgttatgtt attgtttcat tagtaataat cacttaaatg tatcgatgaa
       61 acaggtatga cttaccggga gtgattgttt ctgagtgaac agaggccact ttcgcctgat
      121 aaatgaaaaa tgagcgattt ttgcaagtta gtttttctat agccgagtac gctcagcgcc
      181 tcgcttgatt acaatgaggt tgctgctatg agtcctgata atatcggttc ggctgtcaat
      241 cgcttctcag gttggccatc taatgattct ctaaaaacag ataaagaatc aatgacatca
      301 cccgtgactg tgcaggtaat aaagatgatt gccgccatcc ttgacccaca tcggatagtc
      361 gttggaattg atacacttta tacaacgcag cgcgaaacat ttacctcaga gagtcaacag
      421 ctcattgaat cagcattgca aacacttcat gtgatgaatt taaataatct ggaaacaaat
      481 cgcgatgaag aaagtcacta tctggctttt gaatataacg ttgacacctc tgacagaata
      541 accgtttctg cttataatga aaaaaaacct ggctgctatg gccaatgtct tgtaatacag
      601 gatgacgaaa cagacttatt taacctgata aaaattaaaa ttttcgaagc caggcataat
      661 tatgtcgctc aattgatgag cgatccggaa tttatgttca aattcagtta tagcgctcaa
      721 caaaatagat tagaacctca tcttctggtt cccgcttatt tccctctaaa aacaaacgaa
      781 ccggttacgc aagaggatgt actgttactt tatcgtttct ttaaaatgaa tgatgatttt
      841 aataaattaa catcagatga atacatgagt atcctgacac ctctgatgaa gtgtgagcgt
      901 agcgtccacg acaacaacag gtatgtgacg ggcaaagata cccttcttct ggactatcct
      961 ccttctggta accagataca tttccatgtt ttccctgacg aatcagcaac actagtcctt
     1021 tatttttcaa acaatgacat tgaatgtttc gtctttgaaa gagatattcc gagccagtat
     1081 cgctttttca aaatgtttac caatctggca ctggttatag atcagttaac c
//
LOCUS       DAZFKY010000432         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFKY010000432 DAZFKY010000000
VERSION     DAZFKY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..146)
                     /locus_tag="ACHKCL_005086"
     CDS             complement(<1..146)
                     /locus_tag="ACHKCL_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5765847.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLV"
     gene            complement(371..925)
                     /locus_tag="ACHKCL_005087"
     CDS             complement(371..925)
                     /locus_tag="ACHKCL_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5765848.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(988..>1129)
                     /locus_tag="ACHKCL_005088"
     CDS             complement(988..>1129)
                     /locus_tag="ACHKCL_005088"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5765849.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          276 a          313 c          212 g          328 t
ORIGIN      
        1 ataaccagct gggtcagtat taactggcag cgttcgcgtg aaaggtaagt attctgcgca
       61 atctccccga ctgtcgccgg ttcggtaacg cttaattcat taaacaccac tctggcggtt
      121 tctgtcatat cctgctgttt tagcatgtct ttttcccttt tccggttaac gtgacatacc
      181 aataactctt gtcgaaaaag ccagcaagtt gaaagaccgg tattagcaac caccagcgcg
      241 tttaacgccc cgtgccgttt ttcagtcata aaaaaacccg caaaaagcgg gctctttcaa
      301 atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcttaccg gaattgaagc
      361 agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata accattggtt
      421 tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca caggttctta
      481 aactccattc cggatgtgca tcattcagca attcagccat tttgcgctta gtcatccccc
      541 gccccacata acgctgactc agaacattga gcagcccggg ataacctgcc agtacttcac
      601 caataaccct atcgattatt aacgcctctg aatcggtaca atgtgccagc cagctttttt
      661 gattgccgtt gatcatatcc cgcaaaaaag cctcaagttc aggtttgtcc agacccgctt
      721 ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta gaggtcagta
      781 attggttgaa catatttccc gtcttaccgt cgccaatata cgaccaacgc ccccacatac
      841 gcagtttccc ctggatccag acactttcca gcgttttcag gcgtaaatgc tcaccgcttt
      901 tgcctgtaat ttccgggtat atcatattta tgctcactca ctttcaattt tgtaaatctt
      961 cacgcccagc cgcccaccag aaacgagcta gccgcgcaca atattgattt catcaaactg
     1021 ctcgtcgtct atgagaagtc cggcatgcgt cagcgcatcc agtggtgctt tcaggatatt
     1081 gtccaggtca cgacggcgct tatccggtgg ctctgcaata atctttatc
//
LOCUS       DAZFKY010000433         1128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFKY010000433 DAZFKY010000000
VERSION     DAZFKY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(437..967)
                     /locus_tag="ACHKCL_005089"
     CDS             complement(437..967)
                     /locus_tag="ACHKCL_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5765850.1"
                     /translation="MQVLRAQMAASGGGEITVGGQTVRITYSETDGRFLASGGNNSLL
                     SGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCKFSVNTESLQCPSEAV
                     RCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHPLTREPITSSMIVSQE
                     QCIYDQTKGNFVIKDK"
BASE COUNT          333 a          295 c          188 g          312 t
ORIGIN      
        1 taatgcaatt gaaataaata agaaactcta actctctccc ctcccaggag aaatccacca
       61 atctgccctt ccattacctg ctgacgcatc tccgccatct cattcgcgaa tgaagtcgat
      121 gatatatatg atactaatcc aggcattacc atattccttc atacagataa atatttcctc
      181 tgggcttcat aacaaacatc tccctgacat gacaacaaaa accggagccg gactccggtt
      241 tttgtgaatc cgtcggctat ttcatcccgc caatatttcc ccgtcagcac gccagatttg
      301 cagcggcctc accactcact gtatatgctt tttagctgca tccagtacac caattaccac
      361 atccttatac cctgaacatc tgtagcattt catataagca actggcagtt aacatatagc
      421 ttgggtaata tttatttcac ttatccttta tgacaaagtt tcctttggtt tggtcataaa
      481 tacattgttc ttgacttaca atcattgatg acgttattgg ttctcgtgtt agcgggtgct
      541 tcccaccgtc gcgaaccaaa tgagaaaaag aaaccgaatc aaataaggta caaaccaaag
      601 aaccttctga atttttaaca aacacacctt cttctggttt atccagtata attgggcatc
      661 gaaccgcctc ggatggacac tgaaggctct ccgtattaac agaaaactta cattgtgata
      721 ttttcccctc aatatcacca tgtgattgtg tgttacctga acctgaaacc attcttaaca
      781 ttatatccct gagcgcttca ggaccaccat taagccctgt aagtaataat ccagaaagca
      841 aactgttatt gcccccactc gccagaaagc gaccatccgt ttcactatat gtgatacgga
      901 ccgtctgccc gcctactgta atttctccac cgccactggc ggccatttga gcacgaagaa
      961 cctgcattca ggtggaaaat gaattggatg caatagctga tgttaacggc atgacaaaac
     1021 tccctattta aatattcatt ccaaacacag ggagtcataa caaacatccc ccatacgtga
     1081 caacaaaaaa ccggagccgg actccggttt ttgtgaagct gtcggcta
//
LOCUS       DAZFKY010000434         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFKY010000434 DAZFKY010000000
VERSION     DAZFKY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..147
                     /locus_tag="ACHKCL_005090"
     CDS             <1..147
                     /locus_tag="ACHKCL_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5765851.1"
                     /translation="NKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLA
                     MKNK"
     gene            complement(183..>1127)
                     /locus_tag="ACHKCL_005091"
     CDS             complement(183..>1127)
                     /locus_tag="ACHKCL_005091"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5765852.1"
                     /translation="RIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPN
                     GIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLK
                     LVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPS
                     QFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTL
                     ANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          346 a          222 c          183 g          376 t
ORIGIN      
        1 aataaatatc gctcaccata taatgcatat tatataatct tactatcttt tataggttat
       61 tttgttatcc gtggaaacgt agaacaaatt gaaccaaatt tattaggcgt ttacgccggc
      121 ttattattag cgatgaaaaa caagtaaaaa ataaaaaagg ctgcataatg cggccttttt
      181 atttattcaa accaattatg aataacctct tcgaatcttt gagttacgcc atcccaactg
      241 tagtgatcaa acacaaagtc ctgaccttgc tttgcaactg ctgttaattc aggattcgct
      301 aacgttttta aaatatcgct gcttatggag tctgcagtca taggttcttt taaatggaat
      361 cccgtagtat tttctttgac aaattccgtc atccccccac gcgtactcac taaaacaggc
      421 ttaccagcac ccatagcttc aattgccacc atgcaaaatg gttcctggaa ctgggaagga
      481 atgacgacta aatctgccag tgggtaataa caatgcatct tttcaggcgg tatgcttcct
      541 aacataatgc aacgatcctt caaacgtttt gcaatttccc tgacattgcg ttgatatgca
      601 cctttgtcac ttttactcat ttctgtataa tcaccaatca caactaattt taaatttttg
      661 tgagcagcgg ccaatttttc gaatgattgc atcaacagca atatgccttt gtcagggacg
      721 atacgcccag cataaaaaat aattttctct tcaggggaga tattaagttc gcttctagtg
      781 atcggctgaa aattagattg ataggtttct aaatcaattc catttggtac aatctcaata
      841 tctgcatttg ctaaataaga ttggtaatac tttttcaagt ataaactagg cactatcatt
      901 ttaacatttt gctcaagcaa ttttggttca aatgcattat gcatatgtat aaccactctt
      961 gcctgaggag ctcgcttacg aatttgagtg tatagcttca tgctattatg aactacaata
     1021 acactttctt tcgttatagg aaaatcatgt gcaatattca atattctctg tgaatatgga
     1081 agaggatcaa gtcttgtcca tttttgaaat aaccgcttat atatgcg
//
LOCUS       DAZFKY010000435         1110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFKY010000435 DAZFKY010000000
VERSION     DAZFKY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..996
                     /gene="ehxD"
                     /locus_tag="ACHKCL_005092"
     CDS             <1..996
                     /gene="ehxD"
                     /locus_tag="ACHKCL_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO5765853.1"
                     /translation="EQYRYAILSESIQRNELPEIRITDFPGGEDNAGGEHFQRVSSLI
                     KEQFMTWQNRKNQKQLTLNKKIVERDAALARVSLYEHQVSQEGRKLNDFKYLLNKKAV
                     SQHSVMEQENSYIQAKNEHAVWLAQVSQLEKEIELVREELALETNIFRSEIIEKHRKS
                     TDNIVLLEHELEKNRQRKASSFIKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDI
                     LEVTASVLNKDIGFIQPGQEVVIKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFN
                     VIISVDRNDIQGERKKIPVTAGMTVMAEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT          401 a          164 c          231 g          314 t
ORIGIN      
        1 gaacaatatc gctacgcaat tctttctgaa tcaatacaac ggaatgagct tcctgaaata
       61 aggataacag attttcctgg cggagaagat aatgcgggag gtgaacattt tcagagggtt
      121 agctcattaa taaaagagca attcatgaca tggcagaaca gaaaaaatca aaagcagctc
      181 acattaaata aaaaaatagt tgaacgggat gcagcgcttg ctcgtgttag cctgtatgag
      241 catcaggtat cacaggaagg aagaaaactc aatgatttta agtatttgtt gaataaaaaa
      301 gctgtttctc aacattcagt tatggagcag gaaaatagct atattcaggc aaaaaatgaa
      361 catgcagtct ggcttgcaca ggtttctcaa cttgaaaaag aaatagaact tgtgcgggaa
      421 gaactggcac tggagacgaa tatcttcaga agtgaaatta tcgagaagca cagaaaatca
      481 acagataaca ttgtgttgct ggagcatgag cttgaaaaaa acaggcagag aaaagcatca
      541 tcttttatta aagctcctgt gagtggtact gttcaggagt taaatataca tacagaaggt
      601 ggtgttgtca caacagcaga aacgctgatg attattgtcc ctgataatga tattctcgaa
      661 gtaacagcat ctgtacttaa caaggatatc ggttttatac aacctggaca ggaggtcgtt
      721 attaaagtag atgcatatcc atatacacgt catggttacc ttacagggaa agtaaaaaat
      781 ataactgcag attctgtttc tgttccggat acagggcttg tatttaacgt gattatatct
      841 gttgatcgga atgatataca gggagaaaga aaaaaaatcc ctgttacagc tggaatgact
      901 gttatggcag aaataaaaac cggggtgcgt agtgttatca gctatcttct tagtccatta
      961 aaggaaacta ttaatgaaag tttacgtgaa cgttagtata taaataacat attggctttt
     1021 tatatgatat agatttctga aataatcttg taaagattat ctatgttatt taaacaaact
     1081 atgatacagt ttttatcttt aaaaatagtt
//
LOCUS       DAZFKY010000436         1102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFKY010000436 DAZFKY010000000
VERSION     DAZFKY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..711)
                     /locus_tag="ACHKCL_005093"
     CDS             complement(<1..711)
                     /locus_tag="ACHKCL_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5765854.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIC"
     gene            complement(717..>1102)
                     /locus_tag="ACHKCL_005094"
     CDS             complement(717..>1102)
                     /locus_tag="ACHKCL_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765855.1"
                     /translation="RKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSAS
                     FVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSK
                     CLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          242 a          352 c          289 g          219 t
ORIGIN      
        1 gcaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg
       61 tcgctgccat ttgtcggtat acctctctcg tttgctcagt tgttcaggaa tatggtgcag
      121 cagctcgcca tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca
      181 cagcagcaca tcgcccggtt gtgctgatga caacggcacc tgatacagcc ctgtggcctc
      241 cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc gatgaaaatc
      301 cggcatctca atccccgcca gatgataagc atcccggaac agcgtgtaac agtccgtcac
      361 cccgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat gaatcgtccc
      421 ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt cggcctcact
      481 cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatttc
      541 tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac cggagatatt
      601 cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc acgactccgc
      661 tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca tgggatttac
      721 tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcggaactta
      781 caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg ctggtcatat
      841 tcatccgcga ccgccggacc gtgataaccg cactcatcgc cgcgataggt ccaggtgcag
      901 gtgttggcca gcatgatacg tcccggaaaa acagcgccgt ccgtttccgt cggcgtggac
      961 agtacaaaag aggcactcac cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg
     1021 atcacctcct gctccggatc ggcgtcgctg tttccgttga cgaagttcac cgcatccaga
     1081 aaacgggcgt aaaccttacg cc
//
LOCUS       DAZFKY010000437         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFKY010000437 DAZFKY010000000
VERSION     DAZFKY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..464
                     /locus_tag="ACHKCL_005095"
                     /pseudo
     CDS             1..464
                     /locus_tag="ACHKCL_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            461..787
                     /locus_tag="ACHKCL_005096"
     CDS             461..787
                     /locus_tag="ACHKCL_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5765856.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            797..>1098
                     /locus_tag="ACHKCL_005097"
     CDS             797..>1098
                     /locus_tag="ACHKCL_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO5765857.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDA"
BASE COUNT          232 a          258 c          380 g          228 t
ORIGIN      
        1 ctggaggcgg tgagtgccct gccggctccg caggacgggc gtgatgccac ggcactggaa
       61 atactccccg ccattgacga tcaaaaatcc tttccccggg gctcgtatgc cacacaccag
      121 ggtggactct ggcgggcgta tgaaaaaacg tacgggatgc ggggatggga atgcctggtt
      181 gacggggtgg cggatattga cgtcagcatg acgggtgaac ggtcgttctc tgtggtggtc
      241 cggcagagca gtggccagcg tacggaaaaa acattttccc tgccggtgat gctctaccgt
      301 ggtgtgttca gaatcggcga aacttaccac cccggcgata cggtgacgtg gggggctcgt
      361 tgtggcactg caacagtatg accggtgata agcccggaga agctcattca tcaggctgga
      421 ctctggctgc aaaacgtggg cgggatgcag gaggtggaaa gtgacagcat tactgacact
      481 ggaagaaatc aaggcacatc tgcgtgttga ccatgacgcg gatgatgaga tgctgatgga
      541 caaggttcgt caggctaccg ccgtgctgct ggcctacatc cagggcagcc gggataaagt
      601 gattagtgag gacggtgagc tgatccctgg tgaggcatta acccggatga agggggctgc
      661 catgcgactg accgggatgc tgtaccggaa tccggatctt gctgagcggg aagacctcgt
      721 gcagggggaa ctgccgtttt ctgtttccgt gctgatttac gatttgcgtt gtccgacggt
      781 gttatgagga gggggaatgg caatatctgc aggtcgtctg acacagatga tcagtgttct
      841 gaacccggtg ttaacccgta atgctgccgg agaaatgacg gaagaatggg tgtcatgcgg
      901 gaaaattcat gcggatatcc ggggcaggag cagccgggag cggatgcagt ccggtgcgga
      961 aatggcgcag gcggaaatcc gcatctgggt acgcggtcag tctggtcgtg aaatcacggc
     1021 ggcgtcacga cttcatgtgc tgagtggtcc ctggcgtgac catgtcctga atgtcgtcgg
     1081 ggtcccggtt ccggatgc
//
LOCUS       DAZFKY010000438         1081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFKY010000438 DAZFKY010000000
VERSION     DAZFKY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHKCL_005098"
     CDS             complement(<1..220)
                     /locus_tag="ACHKCL_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5765858.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGG"
     gene            complement(231..929)
                     /locus_tag="ACHKCL_005099"
     CDS             complement(231..929)
                     /locus_tag="ACHKCL_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765859.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(929..>1081)
                     /locus_tag="ACHKCL_005100"
     CDS             complement(929..>1081)
                     /locus_tag="ACHKCL_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765860.1"
                     /translation="EFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDFSCT
                     IEQVVN"
BASE COUNT          242 a          382 c          245 g          212 t
ORIGIN      
        1 gaccaccggg atgactgtgg accagtgcca caatctcccc ctgcatctct gcccgcagcc
       61 agtcttccgg tgcaatacga aaatacgcct ccggctctgc agagatattc acacaaggga
      121 tataccgctc cccctccggc gttctcacca cgaagccgca cgactccgct ggcgcacacc
      181 gccgggcatg cgccagaatc gctgattcag tctgtgtcat aaaacaggat ttactgcgaa
      241 agtttattga tggaaaggaa accgccaaaa ttaaccgcca tgccgcgcat ctcacacccg
      301 cgcatgcact tgctgcatct gtccttacgg atatccgtgg tggggttgtc gaactcatcc
      361 gccacagccc cgcccgtgta accacactca tcagagcggt aggtccacat acaggtattc
      421 gccagcatga tgcgaccggg aaacagcgcc ccatccgtct cggtcggtgt agccagcaca
      481 aacgaggccg tcatggctgt cagctgcgac atctgctcca ccacccagcg gtcactcagc
      541 tcctgctccg ggtccgcctc cggattgccc gcaacgaaat tcaccgcatc cagaaaacgg
      601 gcatacaccc ggcggcggac caccgtggcc cccaccagac tctgcaggtc ctccgccatc
      661 ccggtgacaa ggccaaacag attggacacc gtcagtgacg ggcgggcact gctgcccttc
      721 ccgttcatct caaaaccgct gccgtcaatc gggtatgcct gatattgccg cccctgccag
      781 gtgaccgcct cccctttttc attcagctca ttacagaaaa aataccgctc accaccctgc
      841 accgtcaggt cgatttccca gagtaccacc cgcggtgact gctctgattt aaccgactcg
      901 ttcagacttt cttcatgaat gtcctgcatc agttcaccac ctgctcaatc gtacaactga
      961 aatcactgta ccgggcgtta tccgtgacgc tccactcccg gcacaccacc ctcaccgtcc
     1021 ggttatgttt cggcggtcgc cacaaaaagg cacggtaacc accatgccac gataaaaact
     1081 c
//
LOCUS       DAZFKY010000439         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFKY010000439 DAZFKY010000000
VERSION     DAZFKY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(55..126)
                     /locus_tag="ACHKCL_005101"
                     /pseudo
     CDS             complement(55..126)
                     /locus_tag="ACHKCL_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            120..1075
                     /locus_tag="ACHKCL_005102"
                     /pseudo
     CDS             120..1075
                     /locus_tag="ACHKCL_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          323 a          259 c          260 g          233 t
ORIGIN      
        1 catacatgac aacaaaaacc ggagccggac tccggttttt gtgaagctat cgggttactt
       61 catttcgcca atattttccc acttcccgtc agcacgcagg atttgcagcg gtcttactac
      121 acactgaccc gaggacgaaa aaagagttcg ggctgggaag agacagaaga gtagcaattt
      181 cagaagcatt cacaccaaca ttgagctttt cagcgaaagt gggcacgaat cattgctgga
      241 cagggttaaa ggcactgact caatcactct tcatacgtgg cttgatcgat atgaaacaat
      301 cctcagctag agggggagca agccgaaaac attactcgac tacgccagca aaatcagggc
      361 aattcgaaga aaattgccgg acaaaccgct cactgacata tcaacgaaag aggtggcagc
      421 aatgctaaac acctacgtcg cagaaggtaa agcggtttcc gcaagagtaa tcaggtcaac
      481 ccttgttgac gtttttcgag gggcaatagc cgaggggcat gtggcaacga atccagtaac
      541 aacaacccgt gcagcaaagt cagaagtaag gcgctcaagg ctgacagcta atgagtatgt
      601 cgctatttac catgctgctg agcacctccc catctggctg aggctgtcaa tggatttagc
      661 tgtcgttaca gggcagagag tgggcgattt gtgcagaatg aagtggtcag acataaacga
      721 tggtcatctt cacattggac agagtaaaac aggagccaaa attgccattc cgctggctct
      781 aaccattgac gcactcgaca tctcactggt tgatacacta cagaaatgca gggaggccag
      841 cagcagtgaa acaataatcg catcaaccta tcacgaacca ctttctccag ccacagtatc
      901 acggtattta acaaaggcgc gaaatgcatc cgggatctcg tttgatggag acccaccgac
      961 atttcatgaa ctacgtagtc tgtccgcgag gctatatcgg aaccagattg gctacaagtt
     1021 tgcacaacgt cttcttggac ataaatctga ttcaatggcg gcgcattata gggac
//
LOCUS       DAZFKY010000440         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFKY010000440 DAZFKY010000000
VERSION     DAZFKY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..137)
                     /locus_tag="ACHKCL_005103"
     CDS             complement(<1..137)
                     /locus_tag="ACHKCL_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765861.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VS"
     gene            complement(134..580)
                     /locus_tag="ACHKCL_005104"
     CDS             complement(134..580)
                     /locus_tag="ACHKCL_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5765862.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(577..927)
                     /locus_tag="ACHKCL_005105"
     CDS             complement(577..927)
                     /locus_tag="ACHKCL_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5765863.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(937..>1049)
                     /locus_tag="ACHKCL_005106"
     CDS             complement(937..>1049)
                     /locus_tag="ACHKCL_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765864.1"
                     /translation="MLYRNPDLAEREELIQGELPFSVSVLIYDLRCPTVL"
BASE COUNT          226 a          371 c          247 g          205 t
ORIGIN      
        1 gacaccgatg aaatcaggct gaaaaccacc cacggaagcg ccaccgacgg cctgccatcc
       61 agcaggggga ccacatacgg gtacacctgc ccgccggcaa gatgcgccag atgaggatac
      121 aaatccgcct ccgtcatcgt ctcagtacct catcaatggc ccggttcatc cgcgcaatcg
      181 ccacctgtgc cgcctgttca ctgcgcacat caaatgccgg gcgcacaaac gggtgcggtg
      241 gcatattcac ggtccccatt tccacaaacc gccagtagaa agcgttgcgc gggttatccg
      301 ccttcatggt gttatcgctg ttaccggtgt ccggattaac accccggata tgcacaccgg
      361 attccatccc gccatcgcgg gagcgccggg aaaggaccac cacattgcgg cgcagttttc
      421 ccctgcgtac cggtgcccgt gacaccactt cttctttcag cacattcgca cccgcacggg
      481 ttgcctcacg cagcacccgg ttattttctg caccactcag aagctgcaaa tcgcggctga
      541 tgtcctccag ccccgaaaaa tccagcaggg tttcgatcat ttttcccctc ccagccgaca
      601 gagaatttcc agacgtccgc cggtcgcatc cggcacgggc agcccgacaa cgttcaggat
      661 ccggtcacgc cagggaccac tcagcacatg aagtcgtgac gctgccgtga tttcccgacc
      721 ggactgaccg cgcacccaga tgcggatttc cgcctgcgcc atttccgcac cggactgcat
      781 ccgctcccgg ctgctcctgc cccggatatc cgcatgaatt ttcccgcatg acacccattc
      841 ttccgtcatt tctccggcag cgttacgggt taacaccggg ttcagaacac ttatcatctg
      901 tgtcagacga cctgcagata ttgccattcc ccctcctcat aacaccgtcg gacaacgcaa
      961 atcgtaaatc agcacggaaa cagaaaacgg cagctccccc tgaatcagtt cttcccgctc
     1021 cgcaagatcc ggattccggt acagcatcc
//
LOCUS       DAZFKY010000441         1042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFKY010000441 DAZFKY010000000
VERSION     DAZFKY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..615
                     /locus_tag="ACHKCL_005107"
     CDS             <1..615
                     /locus_tag="ACHKCL_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5765865.1"
                     /translation="GQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPIPGKQ
                     SLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIAITQWAWN
                     EFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAELFGGEGYEYKELALLVGVTTKNWSK
                     TFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKAAFSKQSVAKVD"
     gene            826..901
                     /locus_tag="ACHKCL_005108"
     tRNA            826..901
                     /locus_tag="ACHKCL_005108"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:859..861,aa:Ile,seq:cat)
     gene            909..985
                     /locus_tag="ACHKCL_005109"
     tRNA            909..985
                     /locus_tag="ACHKCL_005109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:943..945,aa:Arg,seq:tcg)
BASE COUNT          291 a          212 c          277 g          262 t
ORIGIN      
        1 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
       61 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
      121 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
      181 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
      241 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
      301 tggaatgaat ttaatactca atccggtacc agaaaaattg cagggaaaac gcaggaacgc
      361 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
      421 ggttatgaat acaaggagct ggcattactg gtgggagtga caactaaaaa ctggtccaaa
      481 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
      541 ttattgtttg taatgagaac gcgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
      601 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
      661 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt tttttgtgcc acttatctcg
      721 gatagacatg gtgaatgcgc tggtggagga agtaagggta atttttaacc aggtgattct
      781 tgaatgcttg caacattgat ttcgtaacgt tattatcctg cgcccggccc tttagctcag
      841 tggtgagagc gagcgactca taatcgccag gccgctggtt caaatccagc aagggccacc
      901 atcacatacc gccattagct catcgggata gaacgccagc cttcgaagct ggtttcgcgg
      961 ggttcgagtc tccgatggcg gtccattatc ggtattctgc gtttttagct cagcaggaca
     1021 gagcaattgc cttctaagca at
//
LOCUS       DAZFKY010000442         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFKY010000442 DAZFKY010000000
VERSION     DAZFKY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..492
                     /gene="nleG7"
                     /locus_tag="ACHKCL_005110"
     CDS             <1..492
                     /gene="nleG7"
                     /locus_tag="ACHKCL_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO5765866.1"
                     /translation="VNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQL
                     SSNIGWKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNS
                     NICTLYDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQ
                     HCK"
     gene            642..>1034
                     /locus_tag="ACHKCL_005111"
     CDS             642..>1034
                     /locus_tag="ACHKCL_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO5765867.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          336 a          233 c          202 g          263 t
ORIGIN      
        1 gtaaatcctg tatgcagtaa tcatttctcc atatttatgg cgagacaaaa cactattgct
       61 cgcaacctgg aacagcagat caacaacgga cgaagttttg cacaaatatc tcaggatttt
      121 atgcttcaat tatcttcaaa tataggatgg aaaaaagggg ccgaaaacgc ccttaaaaat
      181 aaaatccatt ctcattcatt tgttgtaaat cctgatgaat tctcttgcga cacacaattt
      241 cttaagtgcc caataacatt atgcgttcca gaaaaagggg tttttgtcaa gaatgcactg
      301 aactccaaca tatgcactct ttatgataag tctgcgttca tgaatctcac aagagaacat
      361 ctaccccacc ctctcagcag ggaaaagata gtaaaagaaa tgattattga aaggaatatg
      421 tgttattttg acaccataag tcagcatttc ataattatgg atgcagacca acagaaacag
      481 cattgtaaat aaaatgtaat aattacatac tattagtgat tctcatgcat cgtaagcggc
      541 tcgccagaac cgtattgata tttactgagg agcctgtaca tagatttgtg taattgcctg
      601 attttgatat gttcaatcca acatcaaaag caggttaatt tatggacgaa aaacagttgc
      661 aggctctggc taacgaactg gccaaaaatc tcaaaacccc tgaagatctc agccagttcg
      721 atcgcctgct gaagaaaatc agcgttgagg cagctctcaa cgctgaaatg tcccaccatc
      781 tgggctacga taaaaaccag cctaaaccgg gtgccaactc ccgcaatggc tattccacaa
      841 agaccgttat caccggtgat ggccacctgg aactacgcac tccgcgcgat cgtgatggct
      901 cttttgaacc gcaacttgtg aagaaaaacc agacccggat caccgggatg gataaccaga
      961 tcctgtcgtt gtacgccaaa ggcctgacaa cccgcgaaat agcggctgcg tttaaagagc
     1021 tgtatgacgc tgat
//
LOCUS       DAZFKY010000443         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFKY010000443 DAZFKY010000000
VERSION     DAZFKY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..959)
                     /locus_tag="ACHKCL_005112"
     CDS             complement(<1..959)
                     /locus_tag="ACHKCL_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765868.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQ"
     gene            complement(905..>1027)
                     /locus_tag="ACHKCL_005113"
     CDS             complement(905..>1027)
                     /locus_tag="ACHKCL_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765869.1"
                     /translation="KAEYITVRRPAGQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          204 a          324 c          258 g          241 t
ORIGIN      
        1 cactgggaat aatactgctg ctccagcgcc tccacgttgt cactggaagg cggttgtccc
       61 acgccaatct tgtaggccgg gacacggaac accgaacaga caatttcagc ggtcatcttc
      121 agttgttcca ccgtctgcgc atccaccggt gaaaacgtcg tggggttgta ttttgccccg
      181 ttgctcagaa tggccgtttt ccccgcattt tcgcctgtat acccgctgtc ccagttgctc
      241 ttcagttttt tcgcattttc ttccgtaata ctgccgggga tctcaatcac cccggacggc
      301 ctgccgccat ttctgaaaaa agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc
      361 gccagcccgg cggcatacac cggcggcaat cctataagcg gatgaaaaaa acagttaaac
      421 cggtcgtgga tcacttcccg ggcaggcacc gtcaccgcct ccgtgatccc gcagttccgg
      481 tccggcgtga tgcggtagaa cacctcgccg tcatccgcca ccagaggttc aacccggctc
      541 cagtccagaa tacgcagttc tttgatctgc ccccgggcat tacggatttt cagcaccacc
      601 gtattgccat gacgcagttt ggcgttcagc cacagttcaa aaaactggat gcggttctgc
      661 tgggcgttgg gacgacgaca gaggcgggca atatcccccc ggcgcgtttc cctgcgtatc
      721 ccatgcgcat ccgtctgcat aagacgcagc cgcattttgg cgatatcctg ggatatcagc
      781 gaaatacatg caaacaccgc atgaaaggag aggacggctt caggatcggc tttcacgccc
      841 tgctgccagg cgccggaaaa gggctcagcc accgcctgaa acaggctggt ccagcccgcc
      901 tctcttacgt cacgtcctga tttctggttt tttcgggttc gccgtaaaag gttccacatt
      961 cgccatgctc cgcatcacgt ttctttttct gacctgccgg acgtcgcacc gtgatgtact
     1021 ccgcctt
//
LOCUS       DAZFKY010000444         1025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFKY010000444 DAZFKY010000000
VERSION     DAZFKY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..544)
                     /locus_tag="ACHKCL_005114"
     CDS             complement(<1..544)
                     /locus_tag="ACHKCL_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5765870.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQAD"
     gene            complement(525..950)
                     /locus_tag="ACHKCL_005115"
     CDS             complement(525..950)
                     /locus_tag="ACHKCL_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5765871.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          220 a          336 c          249 g          220 t
ORIGIN      
        1 ggtcagcctg ctctgcctgc caggcggcgg cagcaagcgc ggccagtacg gcaaccccgc
       61 cacctgccgc cacacgggcc accgacataa atcgtcccag ctcaccggca ttccgggcat
      121 tttcagccag tgcatttgcc gtatctgaca gcgactcctc tgatgattca gaggcatccc
      181 tgatcccgag aagttcctcc ttcagcaggg taagcaggct gagcggtcca ccgaatgaat
      241 cgctgatctg ccccccctgc tgcagcatga taaggaaggg attctgacca ccggcaagct
      301 gagtgacaat atccgtgaac tgtgcgggca gtgtgcgcat ggcagcctta tactgtccga
      361 ctgatatccc ggctttttgt gccgccagcg cctgtcggct cagcccctgt tcaacagtac
      421 tggcggtttt tctggcatcc gcctccagac tggaaaaatg acggcgtacc cgggccatct
      481 gttcatcaaa ccgggccgca tccagactca aatcaataac cagatcaccc gctggctggg
      541 acatatctca cacctccgga aatccccgct gaagccatca ttaatgcaac atcatcctcg
      601 ctgacatcca ccacatccgc agaaggtgaa atatcgcgcc ctccgtcccc tccgaaccgg
      661 acgcctccgg caactcctgt cgctttctgc atcagcattt cttcctcgtc cggcatctcc
      721 gtctgctctt cctcacacgc tggagcaagc agactgaaat ccgccggatg catatccgga
      781 tcgccaaaaa acaggctgag tacggcgtac atcagcccgg aaaaatgagc gtccagttgg
      841 gtatcctgaa aataatgcgt gcagtaaaaa cgtcgccagt cggcatattc ggtggatgtc
      901 atcccggcaa gcatggcgcg ccagtcgggt ctccccatct ctcgcgccag tttcaggaca
      961 aagttcagct cgccttcgaa tgcttttttg atgttaccga ctcagtcgct tctgctttac
     1021 cggtt
//
LOCUS       DAZFKY010000445         1021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFKY010000445 DAZFKY010000000
VERSION     DAZFKY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            157..486
                     /locus_tag="ACHKCL_005116"
                     /pseudo
     CDS             157..486
                     /locus_tag="ACHKCL_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(624..>1021)
                     /gene="espR4"
                     /locus_tag="ACHKCL_005117"
     CDS             complement(624..>1021)
                     /gene="espR4"
                     /locus_tag="ACHKCL_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFO5765872.1"
                     /translation="IDSFPPKAKKIFISHNKLSETPAIPDTAKVFDCGYNKIQEIRYF
                     PKNLKEARIGYNNIEVVPAIPGNLKILFMECNPIKEAFLMPWTLTGICYEISQRKYIV
                     TNPADYDKYSDMVKKHVIDGEELIIKYFM"
BASE COUNT          287 a          218 c          207 g          309 t
ORIGIN      
        1 tctgatgatc agagggttta tcgcgtatac ggatcgtggg tggcagaaaa ctttcagaat
       61 caggtttcca ttttaaacca gaaattgagt gagtttgccc catccataac cccacgcggt
      121 gagatcggat gtcattaaac aagccaggtc aacaggtcag tatgggaagg cgaacaactc
      181 gcaggaatgg gtgcgctaaa gttgctggat gataaacatg gcgaactgaa atcaatgcgt
      241 accgcgccaa attatttacg tcggggtgtc gccagtctga ttttacgcca cattttgcag
      301 gtcgcccatg acagatgcct tcatcgcctg agcttagaaa cgggtacaca ggctggattt
      361 acggcctgcc atcaacttta tttgaagcat ggtttcgttg attgcgaacc gtttgctgat
      421 tatcaatttg atccacacag tcgatttttg tcattgacgc tatgcgaaga taatgaattg
      481 ccatgagcca gacgcagcgc attcttgcat tcgacgtgct gcgcctttat ttatcaccag
      541 caggaaacgc cttgtccata gacgctcctt ccacatgcgt cacaagaaac ctctattcca
      601 gtgacacaat tacgcctaat taattacata aaatatttaa ttatgagttc ctcaccatct
      661 attacatgct ttttaaccat atcggaatat ttatcataat cggctggatt cgtaacaata
      721 tattttcgct gcgatatttc atagcagatc cctgtcagag tccatggcat taaaaatgcc
      781 tctttaatag gattacattc cataaaaagt atttttaaat tgccaggtat cgcaggaaca
      841 acctcaatat tattatatcc aatacgcgct tctttcagat ttttggggaa gtatcgaatt
      901 tcttgtatct tattatagcc acaatcaaaa accttagcgg tgtctggtat tgctggtgtt
      961 tccgatagct tattgtgact aataaaaatt ttcttagcct ttggcggaaa tgagtcaata
     1021 t
//
LOCUS       DAZFKY010000446          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFKY010000446 DAZFKY010000000
VERSION     DAZFKY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..241
                     /locus_tag="ACHKCL_005118"
     CDS             23..241
                     /locus_tag="ACHKCL_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5765873.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            243..800
                     /locus_tag="ACHKCL_005119"
     CDS             243..800
                     /locus_tag="ACHKCL_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5765874.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            complement(816..>998)
                     /locus_tag="ACHKCL_005120"
     CDS             complement(816..>998)
                     /locus_tag="ACHKCL_005120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765875.1"
                     /translation="GRVMLTGAFLYLPLVFMPEADSLKHPQQFYLTILFPQTVFIPSG
                     KSNTPPAPPPSQFLPL"
BASE COUNT          288 a          218 c          258 g          234 t
ORIGIN      
        1 ccgcccgaaa gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag
       61 cagaacaaca gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc
      121 gctattactg gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga
      181 tgtgacatgg gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg
      241 atatgagcac tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga
      301 gcgactcggc gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg
      361 cgctggcatc actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc
      421 ttacctgcca tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg
      481 tcgccatgct tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc
      541 gggaaatctc ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta
      601 ctgagcgaat gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg
      661 ctgggccatt aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag
      721 cagttagtcc gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc
      781 cgcaggaggt gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc
      841 ggtggcgctg gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg
      901 atagttaagt agaattgctg cgggtgcttg aggctatctg cctcgggcat gaacaccaac
      961 ggcagataga gaaaagcccc agttaacatt acgcgtcc
//
LOCUS       DAZFKY010000447          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFKY010000447 DAZFKY010000000
VERSION     DAZFKY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..275)
                     /locus_tag="ACHKCL_005121"
     CDS             complement(<1..275)
                     /locus_tag="ACHKCL_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5765876.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKI"
     gene            complement(363..905)
                     /locus_tag="ACHKCL_005122"
     CDS             complement(363..905)
                     /locus_tag="ACHKCL_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5765877.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          300 a          210 c          180 g          301 t
ORIGIN      
        1 atgattttaa tttttatttg ttgcccagaa ttggccgcta ctttcactac tggtggcgtt
       61 aacatgaaag gaacctgaat cttttctggc ggcagagacg tgtcgccatc atcaatccac
      121 gcctgcatca gcgaagagcg gtttccctga ttcatcagtt gtaccatgac ctctttctgc
      181 tctgccgggt aaatgatacg ggtcccgaaa ataaccattc cggcatgcac ggggaatatc
      241 atagaagaga tgagtaaaga taataaccct ttcatcttaa tgcctcttat atttatgctg
      301 gccgccatga atatggcggc cattttaact tactgaaaaa acaagatgat tattttaaat
      361 atttaatcct gggcaacacg cataaccaat gaagagttaa ctgtaccaga tgcaacacca
      421 ctgtcagtca acttcagata ttttgcctta aacggtattg catattgtcc atcagcagtt
      481 ttataacttt caatattaaa tggtgcggaa atatccagta cgtcactgct atctcccaaa
      541 tagagatagt atccaactgt gtcattacta ctggatattg ccttatcatt accaagaatt
      601 ttctcgttgg tgatcaacga aagttttacg ccatcaatag tcttcggaca agtaattttc
      661 agcatactgc tggttacacc aaccgcgcca tcataagttg aatatttcgc tttaccatca
      721 aatacagtgg tgggtacagt cggcatatta atactgactg aatcaccgcc agcgacaaat
      781 gtacatggtg attctgcaat attaccttta atggttaaag ttccggtatc tacttcagca
      841 gcgaaaatac cagcactcat aaatacagaa gataagacag cgacagcaat aattgaatgt
      901 ttcatgatgc attccttaat aagtttattt tgttttgtta agagtctgta tctcttggaa
      961 acaaatttct cacatcaaaa aggttgccgc a
//
LOCUS       DAZFKY010000448          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFKY010000448 DAZFKY010000000
VERSION     DAZFKY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..>991
                     /gene="tdcA"
                     /locus_tag="ACHKCL_005123"
     CDS             104..>991
                     /gene="tdcA"
                     /locus_tag="ACHKCL_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5765878.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKE"
BASE COUNT          273 a          189 c          228 g          301 t
ORIGIN      
        1 cctggaaagt attgaaagtt aatttgtgag tggtcgcaca tatcctgttc atttcatttt
       61 gatacacttc atgccgtcaa tgaggtaatt aacgtaggtc gttatgagca ctattcttct
      121 tccgaaaacg cagcacctgg tagtctttca ggaagtcatt agaagtggtt ctatcggctc
      181 ggctgcaaaa gaattagggt taactcaacc ggccgtcagt aaaatcatta acgatattga
      241 agattatttt ggtgtggaat tagtggtgcg gaaaaatacc ggagtaacat taacacctgc
      301 cggtcaattg ttactctctc gttccgaatc cattacccgt gaaatgaaaa atatggttaa
      361 tgagataagc ggtatgtctt ctgaggcggt ggtggaagtc tcatttggtt ttccttcatt
      421 gattggtttt acttttatgt cagggatgat caacaagttc aaagaggtgt tcccgaaagc
      481 gcaggtttct atgtatgaag cgcaactgtc ttcgttctta ccggcaatcc gcgacggtcg
      541 acttgatttt gcgattggta cgttaagcgc agaaatgaag cttcaggatt tacatgttga
      601 gccgctgttc gagtccgagt ttgtgctggt agccagtaag tcccgaacat gcaccggcac
      661 caccacgctg gagtcgttga agaacgaaca gtgggtgttg ccacaaacta atatggggta
      721 ctacagcgaa ctgcttacta cgttacaaag aaatggcatc agtattgaaa acatcgttaa
      781 aaccgactca gtcgtgacaa tttataatct tgttctcaat gctgatttct taactgtaat
      841 tccttgtgat atgacgtctc cttttggttc taatcaattt attactattc cggttgaaga
      901 aacattacct gtggcacaat atgccgcggt atggtcgaaa aattatcgta ttaaaaaagc
      961 agcatcggtt ttggtggaat tagccaaaga g
//
LOCUS       DAZFKY010000449          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFKY010000449 DAZFKY010000000
VERSION     DAZFKY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>991
                     /locus_tag="ACHKCL_005124"
     CDS             <1..>991
                     /locus_tag="ACHKCL_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5765879.1"
                     /translation="VSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALI
                     PFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRT
                     RLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIF
                     QELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWG
                     HRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVEL
                     LTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVII"
BASE COUNT          257 a          203 c          212 g          319 t
ORIGIN      
        1 tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct cgctggcgct
       61 cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaactg gcattctgtt
      121 gctggcgctg atcccttttt tagtttttcc ccttctggca cacaatctga gcagttataa
      181 cgctgactat tctttgtatg tcgtcgatta ctgtccggac aatcatactg cgacctgggg
      241 aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct ttattatcat
      301 gcgtacacga ttagccagcg aactatggcc gttaatagca ttattatgtc tggcatcgct
      361 atgctgcaac ttacttctac tgactctgga tgagtataat tataccatct ggtatatcag
      421 tcgcgggatt gaagtctcca gtaaactgtt tgttgtgtct tttctgattt ataacatttt
      481 tcaggagctg caactctcca gcaaactggc agttcatgat gtgctgacca atatttataa
      541 tcggcgctac tttttcaaca gcgtagagtc attattgtcg cgacctgttg ttaaggactt
      601 ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat ggggacatcg
      661 tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca tccgccccga
      721 tgatatttta gcgcgactgg agggtgaggt gtttggcttg ctatttaccg aactcaatag
      781 tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt
      841 tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacag ttttttcaac
      901 gggtgacacg cgtaatatct cgcttgtcat gacggaagca gataaagcct tacgcgaagc
      961 gaaaagcgag gggggcaaca aagtgattat t
//
LOCUS       DAZFKY010000450          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFKY010000450 DAZFKY010000000
VERSION     DAZFKY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..817
                     /gene="gadX"
                     /locus_tag="ACHKCL_005125"
     CDS             <1..817
                     /gene="gadX"
                     /locus_tag="ACHKCL_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5765880.1"
                     /translation="LHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKC
                     VENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKN
                     LRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLAR
                     IASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSV
                     SYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          284 a          181 c          216 g          291 t
ORIGIN      
        1 attacatggg aattgtctaa ttgcgtacgc aagacataaa tatattctca ccatggttaa
       61 tggtgaatat cgctatttta atggcggtga cctggttttt gcggatgcaa gccaaattcg
      121 agtagataag tgtgttgaaa attttgtatt agtatcaagg gatacgcttt cattatttct
      181 cccgatgctc aaggaggagg cattaaatct tcatgcacat aaaaaagttt cttcattact
      241 cgttcatcac tgtagcagag atattcctgt ttttcaggaa gttgcgcaac tatcgcagaa
      301 taagaatctt cgctatgcag aaatgctacg taaaagagca ttaatctttg cgttgttatc
      361 tgtttttctt gaggatgatc actttatacc gctgcttctg aacgttttac aaccgaacat
      421 gcgaacacga gtttgtacgg ttatcaataa taatatcgct catgagtgga cactagcccg
      481 aatcgccagc gagctgttga tgagtccaag tctgttaaag aaaaaattac gcgaagaaga
      541 gacatcatat tcacagttgc ttactgagtg tagaatgcaa cgtgctttgc aacttattgt
      601 tatacatggt ttttcaatta agcgagtcgc agtgtcctgt ggatatcaca gcgtgtcgta
      661 tttcatttac gtctttcgaa attattatgg gatgacgccc acagagtatc aggagcgatc
      721 ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt gttgcgcaag ggaattttta
      781 cggcactgac tgttctgcgg aaggaataag attatagagt tttactcaga cataaaaaaa
      841 acccggcata ggggaccggg aagaggatag tctgccgtct ccagactaat aaaccgttat
      901 aacactccct gttggcacgg gaaactttgt gctctcagta agttaaatat aacttttact
      961 ggaaataaga tc
//
LOCUS       DAZFKY010000451          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFKY010000451 DAZFKY010000000
VERSION     DAZFKY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>969
                     /gene="sepL"
                     /locus_tag="ACHKCL_005126"
     CDS             <1..>969
                     /gene="sepL"
                     /locus_tag="ACHKCL_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO5765881.1"
                     /translation="SLDFELESQQLTQKNSSNISSPLINLQNELAMITSSSLSETIEG
                     LSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAM
                     FGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGEDDWELALLSWLGVGELNQ
                     EGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSF
                     EDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKDIILNEVLSIIEHVWLTEDWL
                     LESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQRENALLMI"
BASE COUNT          313 a          141 c          205 g          310 t
ORIGIN      
        1 attcattaga ttttgaatta gaatctcagc aattaacgca aaaaaattct tctaatattt
       61 cttcgccatt aataaatttg caaaatgaat tagcaatgat tactagttca tcgctttctg
      121 aaacgattga agggctaagc ctgggatatc gcaaaggtag cgcaaggaaa gaagaggaag
      181 gctcgacaat cgagaagctg ctcaatgata tgcaagagct gcttactctt accgatagtg
      241 ataaaataaa agaactatca ttaaaaaaca gcgggctgct ggaacaacac gatcctactt
      301 tggcgatgtt tggcaacatg ccaaaggggg aaattgttgc gctaatatct tctttattgc
      361 aatctaagtt tgttaagatt gaattaaaaa agaaatatgc caggttatta ttagatttat
      421 taggcgaaga tgattgggag ctggctctgc tttcctggtt aggggtgggt gagttaaatc
      481 aggaaggtat ccagaagatc aagaagcttt atgaaaaggc taaggatgag gattctgaaa
      541 atggcgcctc tttacttgac tggtttatgg agattaagga tcttcccgag cgtgagaagc
      601 acttaaaagt cattattagg gcgctgtcgt tcgatctctc ttatatgtct tcttttgaag
      661 acaaagtaaa gacatcttca attattagtg atttatgcag ggtaatcatt tttttatcac
      721 ttgataacta tgcagatatt atttcgatct ctattaagaa agataaagat atcattttaa
      781 atgaagtgtt atcgattatt gaacatgtct ggctaacaga agactggttg ctggagagtc
      841 cttctcgggt atcgattgtc gaagataaac atatttatta ttttcattta ttgaaagact
      901 tttttacatc attaccagat gcttgcttta ttgatagtga gcagagagag aatgcattat
      961 taatgattg
//
LOCUS       DAZFKY010000452          967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFKY010000452 DAZFKY010000000
VERSION     DAZFKY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHKCL_005127"
     CDS             complement(432..650)
                     /gene="ydfC"
                     /locus_tag="ACHKCL_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5765882.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(722..>967)
                     /locus_tag="ACHKCL_005128"
     CDS             complement(722..>967)
                     /locus_tag="ACHKCL_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765883.1"
                     /translation="AVTKRYAPLLQKVSGECTEDIVNDFVIELRGLIFSYKVTTIFAD
                     GSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          228 a          251 c          232 g          256 t
ORIGIN      
        1 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggcagagca gtcacggagt
       61 aaaactgata ccgccaaacg tcaccagaaa attgataaca gagggcgttg cagcggggtt
      121 gtcacttaag cgtatggtca acctgacaac ccggtgtcct caacggggga aggaataacc
      181 cctccatact taccgccgcg ccatttcgcg gattgccaca accggaagcg cacggtcgac
      241 gaaaatttaa cgacaggcta tctatgaacc agctacctcg ccgtgcgctt tcgcgttatg
      301 gtctgacttt tcagggaaat atcctttcag taaactgtca gtgccgtatg ctcacccgtg
      361 tccggcgcac gcactccacc tcacccgtgg agaactcctt aattaccaac cttagcttcg
      421 ttggttagct attaacgcgg gtatgtaatc attctggcaa tgcttaatgc cgctgctttt
      481 tccagattgg tgatatcctg ctccagagcg gacagatttt cagcctgctt agccctggct
      541 tcattggccc atttcagatc ctgcgctgca ttaattttct ggtgcatcca ctcataaagt
      601 tcatcatcgg tatagtctgg cgcgatgatg acgggttctc gtttctgcat gtcggctcct
      661 tgtggttagc gttgcctgct tttaaccacg tcaggcgagg tggtatcctc tgaggggtct
      721 gttactcgag aggaaattgg ttatgaatac aatcaagttt tcttgcccag aatgtggtgg
      781 cgaagtcttt gacacatcct ttaagccgca gggctctgac agtttcgcgg gagccatctg
      841 caaaaattgt ggtcaccttg taactgaaga tgagtcctcg cagttcgatg acgaaatcgt
      901 tgacaatatc ttcggtgcac tcaccagaga ctttctgaag taaaggcgca taccgcttag
      961 ttaccgc
//
LOCUS       DAZFKY010000453          964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFKY010000453 DAZFKY010000000
VERSION     DAZFKY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            13..774
                     /gene="ydeO"
                     /locus_tag="ACHKCL_005129"
     CDS             13..774
                     /gene="ydeO"
                     /locus_tag="ACHKCL_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5765884.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          297 a          182 c          185 g          300 t
ORIGIN      
        1 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc cttcaacgct
       61 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
      121 gttcgtacgc attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
      181 accattaaaa aatatctaca atgcactaac atccagacag tgacgatgcc tgttcctgcg
      241 aagtttttac gtgcttcgaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
      301 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
      361 atttttgcca catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcg
      421 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
      481 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
      541 acattctcac agattctttt agatgccaga atgcagcacg caaaaaattt gatacgcgta
      601 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
      661 tatgcgttcc gcaaacattt cggcaacagt ccaaagagag tttctaagga gtaccgttgt
      721 caaaggcaca cgggtatgaa tacgggcaac acgatgaatg ctttagctat ttgattattt
      781 gctaacgagt agtcaaccac gcaccctacg taaaaattaa aaatggggca gtcattccct
      841 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat tcatcacata
      901 tttatgcatt tgcataactt gttgcatgat tatttatgat ctcaattcgg cattttgtta
      961 gtaa
//
LOCUS       DAZFKY010000454          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFKY010000454 DAZFKY010000000
VERSION     DAZFKY010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..508
                     /locus_tag="ACHKCL_005130"
     CDS             <1..508
                     /locus_tag="ACHKCL_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5765885.1"
                     /translation="FGRDYRSVMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSP
                     NVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICE
                     LAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFL
                     ENQLQYRG"
     gene            511..>956
                     /locus_tag="ACHKCL_005131"
     CDS             511..>956
                     /locus_tag="ACHKCL_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5765886.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYF"
BASE COUNT          287 a          174 c          201 g          294 t
ORIGIN      
        1 gtttgggcgt gattaccgtt cagtaatgcc aaccaatctt tatggtccaa atgacaattt
       61 tcatccaagt aattctcatg tgattccggc gcttttgcgc cgctttcatg atgctgtgga
      121 aaacaattct ccgaatgttg ttgtttgggg aagtggtact ccaaagcgtg aattcttaca
      181 tgtagatgat atggcttctg caagcattta tgtcatggag atgccatacg atatatggca
      241 aaaaaatact aaagtaatgt tgtctcatat caatattgga acaggtattg actgcacgat
      301 ttgtgagctt gcggaaacaa tagcaaaagt tgtaggttat aaagggcata ttacgttcga
      361 tacaacaaag cccgatggag cccctcgaaa actacttgat gtaacgcttc ttcatcaact
      421 aggttggaat cataaaatta cccttcacaa gggtcttgaa aatacataca actggtttct
      481 tgaaaaccaa cttcaatatc gggggtaata atgtttttac attcccaaga ctttgccaca
      541 attgtaaggt ctactcctct tatttctata gatttgattg tggaaaacga gtttggcgaa
      601 attttgctag gaaaacgaat caaccgcccg gcacagggct attggttcgt tcctggtggt
      661 agggtgttga aagatgaaaa attgcagaca gcctttgaac gattgacaga aattgaacta
      721 ggaattcgtt tgcctctctc tgtgggtaag ttttatggta tctggcagca cttctacgaa
      781 gacaatagta tggggggaga cttttcaacg cattatatag ttatagcatt ccttcttaaa
      841 ttacaaccaa acattttgaa attaccgaag tcacaacata atgcttattg ctggctatcg
      901 cgagcaaagc tgataaatga tgacgatgtg cattataatt gtcgcgcata ttttaa
//
LOCUS       DAZFKY010000455          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFKY010000455 DAZFKY010000000
VERSION     DAZFKY010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..445
                     /locus_tag="ACHKCL_005132"
     CDS             <1..445
                     /locus_tag="ACHKCL_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO5765887.1"
                     /translation="REKKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNV
                     NTLNQNDINNSANNANVRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLH
                     EAQHNDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            405..>956
                     /locus_tag="ACHKCL_005133"
     CDS             405..>956
                     /locus_tag="ACHKCL_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFO5765888.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSKTCYRTIVDIE"
BASE COUNT          339 a          165 c          209 g          243 t
ORIGIN      
        1 tcgtgaaaaa aaataccgcg atggagacaa aataaatgga ccgcaggccc attcattaga
       61 cattactaac gaaagaaggt ttgctgataa cagaaccatg tttactcaac atatcgaaaa
      121 gcaaaggaat gttaatacgc tcaatcagaa tgatataaat aattctgcta ataatgccaa
      181 tgtgcgcgaa aatgaattga catatcagtt tcagcgttgg ggacaaaatc atacggtccg
      241 gattctggaa agtagtgaag ggatacgact aaaaccatca gacacacttg tctccgacag
      301 actccatgaa gcccaacaca atgatgttac agcacaacgt tgggtattga ctgagcaaga
      361 tgaacgacaa ggacaacgac atcagcctca tgaggagcaa gaaaatgaag gcaaattcga
      421 aaacgatcag aaggatgaaa gttaatgttc ggtctgagga aagtaaatcg aaacactcta
      481 cgtttgaaac cacttttcaa aattggaaag aaaatggcga agatgttgca ttattaatgc
      541 ccgaatttag tgctaaatgg ctaccgatag cagaagagag tggcagctgg tctggatggg
      601 tgttgcttcg agaaatattt cccttaattt ctgccgagct cgctgggatg gctttaatgc
      661 cagaaacgga aaggttaata ggggaatggc tcagtttatc cagttccccg ttaaatctaa
      721 agtatcctga actaaaatat aatcgtttgt gcgtaggtaa agtatttgat ggagtattga
      781 gtccagctca accactgata agaatatgga caggggaatt gaatctttgg ttagataaag
      841 tcacagtctg ccaatacgaa aacgctccaa ctttagacaa aaaatcatta tattggccca
      901 ttcattttgt aattggattt agtaaaacat gttatagaac tattgttgat attgaa
//
LOCUS       DAZFKY010000456          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFKY010000456 DAZFKY010000000
VERSION     DAZFKY010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>949
                     /locus_tag="ACHKCL_005134"
     CDS             <1..>949
                     /locus_tag="ACHKCL_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5765889.1"
                     /translation="ILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDL
                     IGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCV
                     GGSADGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTN
                     KPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHAGRV
                     ALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAEVVSD
                     AEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIR"
BASE COUNT          229 a          224 c          314 g          182 t
ORIGIN      
        1 attctgctgg ttccgtgctg tcgtggtggt tcagcgttca ccaccggagc cgatggcaca
       61 tacagtgacg cgagtggtgc ctcggagaat tcaacccgct ggggtgtgga caagccgctg
      121 tataaggacc ttatcggtcg aacaaaagca gcactgaaga agaatccgaa aaatgtgctg
      181 tttgccgtgg tgtggatgca gggggaattt gattttggcg gtacgccggc aaatcacgca
      241 gcacagtttg gtgcgctggt tgataaattc cgtgcagacc tggcggatat ggcaggtcag
      301 tgcgtcggtg gctctgctga cggtgttccc tggatatgcg gggacacgac gtatttctgg
      361 aagcagaaga acgaatcctc gtaccagacg gtgtacggca gctacaaaaa caaaacggaa
      421 aagaatatcc atttcgtacc gttcatgacg gatgagaatg gggtgaatgt gccgacgaac
      481 aaaccggaag aagacccgga cattccgggt atcggatatt acggttcgaa atggcgtgac
      541 agctcagcca cctggacgtc acaggacagg gcgagccatt tcagcgcctg ggcacgccgt
      601 gggattattt ccgaccgtct ggcaacggcg attttgcgcc atgcgggaag agtggcgcta
      661 aacgcggggg catcatcgac agtatcagag gtgcgcccgt catcgccttc cggtgcagaa
      721 gccacaggcg tcacaacact gctctcttac cttgccagcg agtcagaggg aagcctgaaa
      781 gtacagggat ggtcagccag tggcggcagg gcagaagtgg tcagcgatgc ggagggaacc
      841 ggaggtaagg cagtgaagct gaccaaggaa gccggtaaaa gcagctgggt gctggagtac
      901 gccgcgggca acggtgcggc tctgttacag aaaggggggc agattcgct
//
LOCUS       DAZFKY010000457          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFKY010000457 DAZFKY010000000
VERSION     DAZFKY010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..412
                     /gene="ler"
                     /locus_tag="ACHKCL_005135"
     CDS             41..412
                     /gene="ler"
                     /locus_tag="ACHKCL_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO5765890.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            427..645
                     /gene="escE"
                     /locus_tag="ACHKCL_005136"
     CDS             427..645
                     /gene="escE"
                     /locus_tag="ACHKCL_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO5765891.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            658..>939
                     /gene="cesAB"
                     /locus_tag="ACHKCL_005137"
     CDS             658..>939
                     /gene="cesAB"
                     /locus_tag="ACHKCL_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFO5765892.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMN"
BASE COUNT          396 a          135 c          196 g          212 t
ORIGIN      
        1 taatagctta aaatattaaa gcatgcggag attatttatt atgaatatgg aaaataattc
       61 acatacaaca agtccataca ttcagcttat agagcaaatt gcagttctac agcaggaagc
      121 aaagcgactg cgagagcagg aagttcaaag tgtaattgag tcgattcaga agcagattac
      181 ttattacaat ataaccttac aagagctggg atatactaat gtgcctgatg atggactcgc
      241 tcgccggaac tcatcgaaag gtgtttacta ccgcaatgaa gaagggcaga cctggtcggg
      301 cgtaggccga cagccacgct ggcttaaaga agcactgttg aatggaatga agaaagaaga
      361 ttttcttgtg aaggacactg aagaagaaat aataccgctg aaaaatattt aacatgaaat
      421 aattaaatga taacgataac tgagctggaa gatgaaataa taaaaaataa agaagccgca
      481 aatgttttta ttgaaaaaat aaacgacaaa aagaacgaaa tccatgaaaa aatgaaacac
      541 cctttggata aagttaccta caatgaagca aaggaactgc tcattgcgtg tgatgcggca
      601 attaggacta tagagataat gcgaatcagg attaataata aatagaggat gagtattgtg
      661 agccaaacaa gaaataaaga actattagat aaaaaaataa gatcagaaat tgaggcgata
      721 aaaaaaataa tcgccgaatt tgatgtcgta aaagaaagcg ttaatgagtt aagtgaaaaa
      781 gcaaaaacag atcctcaggc ggcagaaaaa ttaaacaaat taattgaggg gtacacatat
      841 ggtgaagagc gaaaattata tgattctgcc ctaagtaaga ttgaaaaact aatagagaca
      901 ctgagtccag caagatctaa aagccaatca acaatgaat
//
LOCUS       DAZFKY010000458          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFKY010000458 DAZFKY010000000
VERSION     DAZFKY010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..525
                     /gene="bet"
                     /locus_tag="ACHKCL_005138"
     CDS             <1..525
                     /gene="bet"
                     /locus_tag="ACHKCL_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5765893.1"
                     /translation="ENQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRREPF
                     KTREGREITGPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAYTAERQ
                     PERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAEAVKAL
                     GFLKQKATEQKVAA"
     gene            522..>936
                     /locus_tag="ACHKCL_005139"
     CDS             522..>936
                     /locus_tag="ACHKCL_005139"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5765894.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRD"
BASE COUNT          255 a          247 c          240 g          194 t
ORIGIN      
        1 gaaaaccagc agtttgatgg catggacttt gagcaggaca atgaatcctg cacatgccgg
       61 atttaccgca aggaccgtaa tcatccgatc tgcgttaccg agtggatgga tgaatgccgc
      121 cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc
      181 aaacggatgt tacgtcataa agccatgatt cagtgtgccc gtctcgcctt cggatttgct
      241 ggtatctatg acaaggatga agccgagcgc attgtcgaaa ataccgcata cactgcagaa
      301 cgtcagccgg aacgcgacat cactccggtt aacgatgaaa ccatgcagga aattaacact
      361 ctgctgatcg ccctggataa aacatgggat gacgacttat tgccgctctg ttcccagata
      421 tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg ccgaagcagt aaaagctctt
      481 ggattcctga aacaaaaagc cactgaacag aaggtggcag catgacaccg gacattatcc
      541 tgcagcgtac tgggatcgac gtgagagctg tcgaacaggg agatgatgcg tggcacaaat
      601 tacggctcgg cgtcatcaca gcttcagaaa ttcacaacgt aatagcaaaa ccccgatcag
      661 gaaagaagtg gcctgacatg aaaatgtcct acttccacac cctgctggct gaggtttgca
      721 ccggtgtggc tccggaagtt aacgctaagg ctctggcctg gggaaaacag tacgagaacg
      781 acgccagaac cctctttgag ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct
      841 atcgcgacga aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggcaacg
      901 gccttgagct gaaatgcccg tttacctccc gggatt
//
LOCUS       DAZFKY010000459          929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFKY010000459 DAZFKY010000000
VERSION     DAZFKY010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            407..>929
                     /gene="espX2"
                     /locus_tag="ACHKCL_005140"
     CDS             407..>929
                     /gene="espX2"
                     /locus_tag="ACHKCL_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO5765895.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RTVNSLNVGEKLND"
BASE COUNT          300 a          148 c          178 g          303 t
ORIGIN      
        1 tctgggaaaa tctggaataa acatgctatt gcataaagag tttgttcatc atctggtaaa
       61 ttattatgag atatgtgatt cagtattagc ttgattctgt ccctgatttt attattttca
      121 ttgtctatag tgtatccgac tatctcccta agcaaaataa agtgtgcagt cgtttcatta
      181 ccgtatatct ttagtagttc taataaacta tcaaaaatcg ctcatttcag cttattgata
      241 gaaagattga ttgccagtct gtttagagcc gtacagcagc aaactttcat aaccaggttg
      301 atcctgaatt tgcagtacga aaagttaaaa atgagcgatt tctgtaagac tccccatttt
      361 gccaggctta tgattcgcag atcattcaag tgcaaaggag cgatgtatgg attgttcaaa
      421 atgcaatggt tatgcgacga tgttattaaa tatggtgcag ggttctgacc cggttaattt
      481 actggaatta catggtttcc ttgaacattt cgcatattat gtttcatttg gcaaatttaa
      541 tgctggccac cagcgctata atgcatttaa aaaattcgtc agtgagattt cggagatcag
      601 cgcaaatgat atcaatatga cgattaaaac cgggcaatcc agacatgaaa atgtgatctc
      661 tataaatatg aatgatgcaa ttcctcgtga tgagaaaggg atcaccgtta gaatagataa
      721 tattaatgga aagaaaaaca actctaattc atcagatgtt tttattcctt atgtaaatac
      781 atttcccgac ctcaaaaata aaatactcag gatgaaaatt gaactgactg aaggtagtgg
      841 tttttctaaa agtctttctg acagtcagat tgagatgcat attctgagga ctgttaattc
      901 gctaaatgtt ggcgagaaat tgaatgatg
//
LOCUS       DAZFKY010000460          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFKY010000460 DAZFKY010000000
VERSION     DAZFKY010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..250)
                     /locus_tag="ACHKCL_005141"
     CDS             complement(<1..250)
                     /locus_tag="ACHKCL_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5765896.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQA"
     gene            complement(309..653)
                     /locus_tag="ACHKCL_005142"
     CDS             complement(309..653)
                     /locus_tag="ACHKCL_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5765897.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(650..>928)
                     /locus_tag="ACHKCL_005143"
     CDS             complement(650..>928)
                     /locus_tag="ACHKCL_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5765898.1"
                     /translation="RRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNA
                     FYWRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          212 a          292 c          236 g          188 t
ORIGIN      
        1 gggcctgatt cagataaaaa ttacccgaca tggaaatctc ggacgacgcc cccagaccgt
       61 tgatgttctc ctgctctgtg gagcagagcg tggtcacatc aatatcctgt ttctgaccgg
      121 cggtgaactg gacttccttg atggtgcagt ccaggcgcag atattccgcc ttctccatgg
      181 tttcagcagt cgccggggca gatgaaatca tcacctgcgt cagctgtgaa cgttcataca
      241 aagcagacat tctgcctcct gataataaaa aacccgcacg cggcgggtta tgggttctgt
      301 tgaaaaaatt acaccgtgac ctgaaactcc agggttgcac ggtaacagcg gttttccgga
      361 atatagtcct gcatttcact gacggatccc ggggccagca gcattatggc ttcacgggcg
      421 tcctgacgta tctgacgcgc ctgcgtcaca gtcccggcat aaacgtctat ctgcaccgac
      481 actgaggact ccgcctgccc gcccatcacg tccgccgaca ccgatgaaat caggctgaaa
      541 accacccacg gaagcgccac cgacggcctg ccatccagca gggggaccac atacgggtac
      601 acctgcccgc cggcaagatg cgccagatga ggatacaaat ccgcctccgt catcgtctca
      661 gtacctcatc aatggcccgg ttcatccgag caatcgccac ctgtgccgcc tgttcactgc
      721 gcacatcaaa tgccgggcgc acaaacgggt gcggtggcat attcacggtc cccatttcca
      781 caaaccgcca gtagaaagca ttgcgcgggt tatccgcctt catggtgtta tcgctgttac
      841 cggtgtccgg attaacaccc cggatatgca caccggattc catcccgcca tcgcgggagc
      901 accgggaaag gaccaccaca ttgcggcg
//
LOCUS       DAZFKY010000461          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFKY010000461 DAZFKY010000000
VERSION     DAZFKY010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>927)
                     /locus_tag="ACHKCL_005144"
     CDS             complement(<1..>927)
                     /locus_tag="ACHKCL_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765899.1"
                     /translation="KFIEFCKSTISNNNLTTSWESNKWKGLYRFTKFNSKNNLNSKEC
                     LDDSFINFAKAYMLHVHSFNKSKTKHSTLSMLKIVEFVLLKINMEANVNYCNNSIYDE
                     CIRIASEKYSKAHAFAIGKELEKLSSFLNDNRMTNSFYLFWVNPIRYRITQSWTGYDS
                     SLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTSVLALLMCAPSRISEILALPAD
                     CEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIPVAKKAISRLKELSSQARLLAA
                     EIQKNYSNSTKGTLKENIPPDLFWYDREKKIKY"
BASE COUNT          298 a          172 c          127 g          330 t
ORIGIN      
        1 atatttgatt ttcttctctc tatcatacca aaagagatca ggaggtatat tttctttaag
       61 ggttcccttc gttgaattag agtaattctt ttgaatttca gcagccaata acctcgcttg
      121 acttgataat tcttttaatc tgctaatagc ctttttagct acaggtatca ttaaagttgg
      181 aatccatttt atattgcctt catacccttt tgctgaaaaa aatcttaaac cgtatctttg
      241 aatgcccttt ccatcacatt ctgtgatttc acaatccgct ggcaaagcta aaatttccga
      301 tatcctactc ggtgcacaca taagtaaagc aagcacagat gtagtaaata tatctcttga
      361 cgataattgt tcatcccgct ttgaaaaaat ctcagcaatc gcaataactg atttgatatc
      421 aggcaatcta gaatggcctt ccagtgaaga atcataacca gtccaagact gagtaatcct
      481 atacctaatt ggatttaccc aaaataaata aaatgagtta gtcatcctat tatcatttaa
      541 aaatgaactc aatttctcaa gttctttccc aatagcaaat gcatgtgctt tagaatactt
      601 ttcagaggct atccttatgc attcatcata gattgaattg ttgcaatagt ttacattagc
      661 ttccatattg attttaagta aaacgaattc gacaatcttc aacattgata atgttgagtg
      721 tttcgtctta gatttattga atgaatgcac atgcaacata tatgctttag caaaattaat
      781 aaagctatca tctaagcact ccttgctgtt taggttgttt tttgagttaa acttagtaaa
      841 cctatataaa cctttccatt tattgctttc ccatgaagtt gttaaattat tattagaaat
      901 agtactctta caaaactcga taaactt
//
LOCUS       DAZFKY010000462          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFKY010000462 DAZFKY010000000
VERSION     DAZFKY010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..286
                     /locus_tag="ACHKCL_005145"
     CDS             86..286
                     /locus_tag="ACHKCL_005145"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO5765900.1"
                     /translation="MALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWKIEGKQPKNS
                     GGNFTMLIAKKDGQVLNVIHTK"
BASE COUNT          321 a          148 c          188 g          265 t
ORIGIN      
        1 attacttatt atgatattgt ttacaatatc acactcagta tttggaaaag aaaatccagt
       61 ggaaatttaa gtaaatagtc gtgaaatggc tttggaatta tcatatgttt acattaaata
      121 tgtatacggg aaagaaaaag cagaatttca gaaaccatat agtattacag atgacaataa
      181 ttgttggaaa atcgagggaa aacaaccgaa gaattcagga ggaaatttta caatgctaat
      241 agcgaaaaaa gatgggcaag tgttgaatgt aatacacaca aaataatcca ctgtacagtc
      301 gattcaaata ttttaccaag gtgtgtttcg taacaatttt gtgtacaata tgactctaca
      361 tatcgacgaa aaaaccactt gatttgacag gaatacattc aaaaaatttc ttttttcatt
      421 tcaggagcga ttttcatctg ttacttgaag tatctgtgag attaagtgac tccccctaac
      481 gtcgtactaa atagtaatta ctggccatac cgggagcagt attcggcgct ccccacatgc
      541 agcagaataa ccgcatcagc gaagatgtat ttttactatc acaatgtgta cggacggttt
      601 atggaaaaga atgaacatta gatacgcgat gcggcggcag gttacacggt tatcactacg
      661 atagccaata ctggctggtg cattaccgcc gtgaacaata ggacgccgta ctgctggaaa
      721 tttcttacct ttataactcc ccgcgccgcc gtatcgggaa acgggaagca atcaagatga
      781 ggctgatata atgtgcggaa tgcctgagta tttttagaat aggggggtaa tatgtttgat
      841 agaatatatc ttggtgatcg agcaataaaa aaattgaatt tgatttatgg agtaaacaaa
      901 ttagaattca ggttaattta at
//
LOCUS       DAZFKY010000463          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFKY010000463 DAZFKY010000000
VERSION     DAZFKY010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..295
                     /locus_tag="ACHKCL_005146"
     CDS             <1..295
                     /locus_tag="ACHKCL_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5765901.1"
                     /translation="HLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPR
                     KNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            557..>922
                     /locus_tag="ACHKCL_005147"
     CDS             557..>922
                     /locus_tag="ACHKCL_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765902.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLD"
BASE COUNT          311 a          150 c          180 g          281 t
ORIGIN      
        1 ccatctaatt agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa
       61 cactactgat ttcagaccag atcctgaaga tatcttcaaa tgtggacaga cgctcgaatg
      121 tggcgacccc cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca
      181 gaatcaacaa tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga
      241 aaataaaaaa ataataggtt cttcctcagg actaagttgt tttcaaccac attaaaactc
      301 tatgagtgct gtggtatatc tttttaatcc tgccagtatt tcatagtttt tcttaatcga
      361 ttgtagaggc atgtactgat gacgcctcta caatctttag ggactttctc tactatgaga
      421 taagaaattt catattatgc tttaaggctg aatgatttgt gcaaaatata attatttttc
      481 catcgaacat gaggtgtatg tcataagatt aaatgataac tatgtattcc tcgcggtgaa
      541 ttatatgagg taaaagatga aaattggaac tgtggcaggt actaacgaca gtacaacgac
      601 aatcgcaaca aatgatatgg tgcaagagca tgttactaac ttcactaaag aactgtttgg
      661 gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa tgctcggtga
      721 ggttctggaa aaagtagatg attggcaaat agaacggttc caacaatcta ttcaagatga
      781 caaaatttca tttaccatac aaacggatta ttcagaaaaa tatagtatgc tatcgggcat
      841 gcgtgctcat attctacgta ggaataacca ttatcagttt attgtgacga ttaatagcaa
      901 aaattacggt tgccctcttg at
//
LOCUS       DAZFKY010000464          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFKY010000464 DAZFKY010000000
VERSION     DAZFKY010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(713..>921)
                     /locus_tag="ACHKCL_005148"
     CDS             complement(713..>921)
                     /locus_tag="ACHKCL_005148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765903.1"
                     /translation="SIYHVICSVTSKINIKNSAKFNIHSTSRLTYDIISQLHLPQNGY
                     FMEHSNFLVAIIKLILRSLAIIPG"
BASE COUNT          276 a          163 c          186 g          296 t
ORIGIN      
        1 tcaattcatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
       61 tttcaggctg aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat
      121 agattgcaag atgccctgtc tcattgtcta tcgtggtgca cctttatacc tgttatacca
      181 gatcgaaaat cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat
      241 gtcgatcttt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct
      301 tcaaaaacta aagatcttcg tttaactatt tgttttataa ataatttatt tagagtctaa
      361 acaagggaag ctttgcaagc taactcagtg agcttggtgt aaatcagtgt ttacccgcca
      421 tcaggctgag cataattctc atcatgaaat atgtttcctg gtttgtggct tgtaactggt
      481 cacttctgaa gtcgatctgg agaggcttat tgatgttggt gttttcagga tgatgtttca
      541 cttagtttgt ttgccgtatc gcccggcgaa tggctgtgat tgaggaaggt ttaagtcgta
      601 gtgaccaaag ctatatttac caacgaatgt agatgaaaaa atcatctcct gcgttccccc
      661 atatctccag gataaaaagg aatgtaacaa tctcatggcg taagctgacg aatcagccag
      721 gaataatcgc tagggaccta agaattagct tgataatagc cactaagaaa ttactgtgtt
      781 ccatgaaata gccattttgt ggcaaatgga gttgactaat aatgtcatat gtgagacggc
      841 tagttgaatg aatattaaat tttgctgaat tttttatgtt gattttactt gttacagaac
      901 atatcacatg atatatagat a
//
LOCUS       DAZFKY010000465          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFKY010000465 DAZFKY010000000
VERSION     DAZFKY010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..372
                     /locus_tag="ACHKCL_005149"
     CDS             <1..372
                     /locus_tag="ACHKCL_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO5765904.1"
                     /translation="NSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHD
                     ISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHK
                     DFAFKPFFTNFNTIERIENYL"
     gene            369..860
                     /locus_tag="ACHKCL_005150"
     CDS             369..860
                     /locus_tag="ACHKCL_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO5765905.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIEQVFNPHNFKK
                     DNF"
BASE COUNT          325 a          167 c          134 g          290 t
ORIGIN      
        1 aattcaactc cctcgacagg ataccaacct cttatcctgc aaagcattaa aaccaaatta
       61 ctttcatcaa gactaaataa atttaatgac ttcgaggaca gttttaatgg cttaggaatc
      121 agtgttcatg atatttcagc tcaaaaaata tctcttttaa gttttcaaaa atacgctata
      181 ggatggagtg caacaattca ttttgtagca caggaccact tcggtcttga tgtaactgat
      241 atcaaaaaca agacttacag caaatataga ttttttcgca tatggttctt tttacaacgg
      301 cataaagact ttgctttcaa accttttttc accaatttta acactatcga aagaatcgaa
      361 aattatctat gaaaagcaaa ctccaaaaaa ttatattatg tttatttctt ctatgctgca
      421 tctataattt atggacattg cgtcctgtac agattttgta tacatactct gatgctggca
      481 attcagtctt tctggtcgtt gatcatctgc catggactga cagcgataaa ataaattggt
      541 atctgaagca tcagaatgaa attaaaaatc aacatcccct accagaaggt agttggcata
      601 cctggtatgt cattgatatc ggtaatggct tcactgacta taaaaaatat atcgaagggc
      661 cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa tgataattgc atcacgaaaa
      721 attacttaat ggttattaat gaataccctt atcgtaacac tcatattggt attaacgatt
      781 ttactgagta tcaattaaca caagaaaata aaatagagca agttttcaat ccgcataact
      841 ttaaaaaaga caatttttag aaataagccg gataaaaaaa tagctttatc cggcactcat
      901 attacaacca gttctt
//
LOCUS       DAZFKY010000466          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFKY010000466 DAZFKY010000000
VERSION     DAZFKY010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            45..845
                     /locus_tag="ACHKCL_005151"
     CDS             45..845
                     /locus_tag="ACHKCL_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765906.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          290 a          177 c          207 g          237 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatatgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg gaagcgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggt ggcagcaaac aggagttgag
      901 tatttctata a
//
LOCUS       DAZFKY010000467          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFKY010000467 DAZFKY010000000
VERSION     DAZFKY010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            209..793
                     /locus_tag="ACHKCL_005152"
     CDS             209..793
                     /locus_tag="ACHKCL_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5765907.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(818..>908)
                     /locus_tag="ACHKCL_005153"
     CDS             complement(818..>908)
                     /locus_tag="ACHKCL_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5765908.1"
                     /translation="QHMGFKLSGRSERDNQGRPYPLLHLYNAM"
BASE COUNT          274 a          184 c          196 g          254 t
ORIGIN      
        1 gattaacctc tatgtagcta ggtttttgta aagaaactgt gcgtcactgt atccaggact
       61 gttatgtgcc aaataaggaa gttgaagtta ccagctctta ctctaaagct ataacgtata
      121 gaaaaaatgc atttgagacg tgtaaaatca tttatttaat cgcaatttat gtaatggcat
      181 aatcaggtcg gtgaaagagg ggagaagaat ggataaaaga gctaaaaatc agatagtcga
      241 tagcgatatt gcacgtttgt tactcaagct cagaaagtct cgaaacttga ctgttacaga
      301 gcttgcccaa cgatctggtg tcagtcaggc gatgatcagc aaagttgaac gaggcacttc
      361 aagccccagt gcaacaatac ttagtcggtt agctaatgca ctgaatatta cgctatcaaa
      421 gctttttgca gagcttgaga tgcaacagaa ttcacttgta cttttggctg accagcaaca
      481 acattggatt gatgaagaaa caggcattac ccgctggtct ttgtccccag cgggtgcatg
      541 tccggaacta ataaaagttg aaatcccccc tatggggcaa ttgactatcc ctgcctctgc
      601 aaatgagcat ctttctggac aaactctatg gatgttaagc ggcagccttg attttcgagt
      661 caatcatcag actcatcatt tacaagccgg tgattgtctt gctcttacct ttgccagcga
      721 gtaccaaatg agaaacccag atagcggcca gagttgttct tacattgtcg ctttcagcca
      781 aaaaaatcca tgatgtaatt ctcctaaaat tatcatgtca cattgcgtta taaagatgta
      841 atagagggta gggtctgcct tgattgtccc gctcggaacg tcctgaaagt ttaaagccca
      901 tatgttga
//
LOCUS       DAZFKY010000468          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFKY010000468 DAZFKY010000000
VERSION     DAZFKY010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            178..441
                     /locus_tag="ACHKCL_005154"
                     /pseudo
     CDS             178..441
                     /locus_tag="ACHKCL_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            481..601
                     /locus_tag="ACHKCL_005155"
                     /pseudo
     CDS             481..601
                     /locus_tag="ACHKCL_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            598..750
                     /locus_tag="ACHKCL_005156"
                     /pseudo
     CDS             598..750
                     /locus_tag="ACHKCL_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          269 a          202 c          211 g          225 t
ORIGIN      
        1 cgttgtgcat gaccgggtaa aagcaacgag gacaacgctg tcgcagtgct gacggaactg
       61 ccgacgtgta cttatcctta tccgccagtg atataagacg gtaattcacc atttggattg
      121 tccgctccac ccaacatgtt gtttccttga ggttctcaca ccagaaagga catcagcatg
      181 ctgagcagag aggactttta catgataaag caaatgcgcc agcaaggtgc gtatattgtt
      241 gatattgcga ctcagattgg ttgctctgaa cgaacggtca gacgttacct gaaataccca
      301 gaacctccgg ccagaaaaac acgccacaaa atggccgaaa tggactatat cgacatgcgt
      361 ctggctgaga atgtctggaa cggcgaagtt atcctggcgg aaatcaaagc gatgggttat
      421 accggtggcc gttccatgtt gcttcgccag ttcaggaaga caccggagca gcgctttacg
      481 caggaacaca gtctgttctt ccggttgctg aaccgtcgat atgaaaaagc gagcattgtg
      541 ctgacgtcca ataaagggtt cgccgactgg gggagagatg ttcggtgata acgtgctggc
      601 aaaatattca cccaacgcat tgagcgtaat aatctgacgc tacgcacccg cattaagcgg
      661 ttgactcgta aaaccatctg cttctcacgc tcagttgaga tccacgaaaa agtgatcgga
      721 gcgtttatcg aaaaacacat gttctactaa ttggatgcac caccgaatac attaatttat
      781 ataacaacat caaaatacat tttaggttgt cgctaatcaa aaattattaa gtgttacata
      841 attagagggt atagatatga ttcatcctgg ctcttcttta gataaagcaa ttaacaatac
      901 aagagtt
//
LOCUS       DAZFKY010000469          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFKY010000469 DAZFKY010000000
VERSION     DAZFKY010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            215..817
                     /gene="fimB"
                     /locus_tag="ACHKCL_005157"
     CDS             215..817
                     /gene="fimB"
                     /locus_tag="ACHKCL_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5765909.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          269 a          165 c          195 g          275 t
ORIGIN      
        1 taaaggtaca tagcatgcaa gagcatagcg tttgtatggc aacgttatta taattaactg
       61 ttgctactcc atttaagttc actcagaaga actggtccac ttacgttagt tattaagcaa
      121 acgttcgctt ttataaacat aatcaggata aaaatgttgg attattgcta acctagcaca
      181 gctagtgcgc gtctgtaatt ataagggaaa aacgatgaag aataaggctg ataacaaaaa
      241 aaggaacttc ctgacccata gtgaaatcga atcactcctt aaagcagcaa ataccgggcc
      301 tcatgcagca cgtaattatt gtctgacttt gctttgtttt attcatggtt tccgggcgag
      361 tgaaatttgt cgattgagga tttcggatat tgatcttaag gcaaagtgta tatatatcca
      421 tcgattaaaa aaaggctttt caacaacgca cccgctattg aacaaagaag ttcaggcttt
      481 aaaaaactgg ttgagtatcc gtacttctta tccgcatgct gagagcgagt gggtattttt
      541 atcgcgtaag gggaatccgc tttctcggca acaattttac catattatct cgacttccgg
      601 tggtaatgcc gggttgtcac tggagattca tccgcacatg ttacgccatt cgtgtggttt
      661 tgctttggcg aatatgggaa tagatacgcg acttatccag gattatcttg ggcatcgcaa
      721 tattcgtcat actgtctggt atactgccag caatgcaggg cgtttttacg gcatctggga
      781 tagagccaga ggacgacagc gtcacgctgt tttatagaga tagttgagat accagggatg
      841 gtgttaaaaa ctacattatt cttacgatat atcaaaaatg attaaccatt tattaattga
      901 tgtt
//
LOCUS       DAZFKY010000470          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFKY010000470 DAZFKY010000000
VERSION     DAZFKY010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..218)
                     /locus_tag="ACHKCL_005158"
     CDS             complement(<1..218)
                     /locus_tag="ACHKCL_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO5765910.1"
                     /translation="MPVTTLSIPSISQLSPARVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRM"
     gene            complement(291..866)
                     /locus_tag="ACHKCL_005159"
     CDS             complement(291..866)
                     /locus_tag="ACHKCL_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5765911.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
BASE COUNT          279 a          212 c          158 g          250 t
ORIGIN      
        1 tccattcgat gctcacgccc caatagccta tccagtaagc ctcctctcac cggttcaact
       61 gaaaatccat ccagtagttg gacatagtga acagaatatt ggccactacc aatggatatt
      121 cttattccac tttcaagtct ggctgcatcc tgcaaagact gtactcttgc aggagataat
      181 tgagatatac ttgggatact taaggtggta acaggcataa aaacctcatt cagtaattca
      241 tatcagtttg caggatgttt cattaaataa aataaagggt aatgttcatt tcaattaccc
      301 tttataacga agtttcctct ggtatcgtca taaatgcatt cttcatgttt tacaattatt
      361 gatgccgtta ttggttcccg ggtcagtggg tggggtaagc cttcaccaac caaacgagaa
      421 aatgcagcgg catcaaataa agtacataca tctgaaccat ctgaattctt cacaaaaata
      481 cctttttcag gctgctccag tgtaattgga cattgaatag cctcaagcgg gcactgaagt
      541 ctctctggac aaacagaaaa cttacattga gatattttgt tctgaatact ctcttgcgat
      601 tgtgcttcac ctgagtccga aagcatactt agcattctct caccaagggc tcgaggacca
      661 ctattaaatc ccaaaagcaa taactcagaa agcaatccgc cattaccgcc actggatgaa
      721 aagcgcccat tagtagaatc atacacaata ctgacagttt caccaccgac tgtaatatct
      781 ccgcgcccat tggctacaat tcgggcacga acaacctcca ccccaagatt aaatgaatgt
      841 gatctaatat ctgaggttaa tggcataaca aagctcccta ttttaatttg aactccaga
//
LOCUS       DAZFKY010000471          893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFKY010000471 DAZFKY010000000
VERSION     DAZFKY010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>893
                     /locus_tag="ACHKCL_005160"
     CDS             <1..>893
                     /locus_tag="ACHKCL_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765912.1"
                     /translation="LDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNY
                     VKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLS
                     NEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQLL
                     YLTNPIDVMNILNKYIYYFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQK
                     TINCVVEIENIVFGGYIYTLNNGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSG
                     KTESVHRTINKLNYEKLPFGF"
BASE COUNT          323 a          158 c          140 g          272 t
ORIGIN      
        1 actcgacatc aggaacacat atcgcccgga taatgagatg gcattttact atcatattca
       61 ttcttttctc actcagatac cggataatga ggatgatata tatcatgctg caaggacata
      121 tctgcgaaat tacgttaagt tatgtttatc cggatacacg ccagcgaatg cgcatttcaa
      181 agatatcttt gatggcgtat atgaattcat tcgtaatatt cgcaaaaaca gtacaccagg
      241 aaaaacaaaa cttatcgcaa ctatcaacac atgcaaagaa acctgtaaac atctgcttta
      301 tttaagtaat gaagacaagg aaaaaataat ttctgactta gataaagttc aggttgcatg
      361 ttattatctc actatattac tggctttcga aagacgaact tcattaacaa gcaccctggc
      421 aactttatat aaaatgctga taagcgaaag agaagtttca gaatatgaat gccagttatt
      481 atatttaacc aacccaatag atgtaatgaa tatactgaac aaatacatat attactttcc
      541 taatgagaac tcaccatttt atacactgaa aattgacagt gcattatcgt gggatgccat
      601 tgacgcaata cgagactata gtatttctga tatttatctt tatcctgaac aaaaaacaat
      661 aaattgtgtc gttgagattg aaaacattgt ctttggcggt tacatttata cattgaacaa
      721 cggcgtcaca ttacaaaaca tagaaaactc tttaaaagat tcttcatgcc attatgtctt
      781 aaatggctat acagaatttg ttaactgttt gagacaactt acttcaggaa agactgaaag
      841 tgttcatcgc accatcaata aactgaacta tgagaaatta ccttttggat tta
//
LOCUS       DAZFKY010000472          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFKY010000472 DAZFKY010000000
VERSION     DAZFKY010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(61..759)
                     /locus_tag="ACHKCL_005161"
     CDS             complement(61..759)
                     /locus_tag="ACHKCL_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765913.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(759..>890)
                     /locus_tag="ACHKCL_005162"
     CDS             complement(759..>890)
                     /locus_tag="ACHKCL_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765914.1"
                     /translation="GWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT          195 a          311 c          204 g          180 t
ORIGIN      
        1 ggcgcacacc gccgggcatg cgccagaatc gctgattcag tctgtgtcat aaaccgggat
       61 ttactgcgaa agtttattaa tggaaaggaa accgccaaaa ttagccacca tgccgcgcat
      121 ctcacacccg cgcatgcact tgctgcatct gtccttacgg atatcggtgg tgggtttatc
      181 gaactcatcc gccacagccc cgcccgtgta accacactca tcagagcggt aggtccacat
      241 acaggtattc gccagcataa tgcgaccggg aaacagcgct ccgtccgtct ccgtcggtgt
      301 ggccagcaca aacgaggctg tcatggccgt cagctctgac atctgctcca ccacccagcg
      361 gtcgctcagc tcctgctccg ggtccgcttc cggattgccc gccacaaaat tcaccgcatc
      421 cagaaaacgg gcatacaccc ggcggcggac caccgtggcc cccaccaggc tctgcaggtc
      481 ctccgccatc ccggtgacca gaccaaacag attggacacc gtcagcgacg gtctggcact
      541 gctgcccttc ccgttcatct caaaaccgct gccgtcaatc gggtatacct gatattgccg
      601 cccctgccag gtgaccgcct cccctttttc attcagctca ttacagaaaa aataccgctc
      661 accgccctgc accgtcaggt caatttccct gagcaccacc cgcggtgact gctctgattt
      721 aaccgactcg ttcagacttt cttcgtgaat atcctgcatc agttcaccac ctgcttaaac
      781 tcggcactga attcaacccg cagcatgcga acccgtgatg accaggcggc acaggtcacc
      841 tttatctgcc gccaggcata aggcggtgtc cacagaaacg ccttccagcc
//
LOCUS       DAZFKY010000473          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFKY010000473 DAZFKY010000000
VERSION     DAZFKY010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>885)
                     /locus_tag="ACHKCL_005163"
     CDS             complement(<1..>885)
                     /locus_tag="ACHKCL_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765915.1"
                     /translation="RRTRKNQKSGRDVREVGWRSLFQAVAEPFAGAWQQGVKADPETV
                     LSFHAVFSCISLISQDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFF
                     ELWLNSKLRHGNTVVLKIRTPRGQIKELRILDWNRVEPLVADDGEVFYRITPDRNCGI
                     TESVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSG
                     VIEVPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLK
                     MTAEIVCSVFRVPAYKIGV"
BASE COUNT          188 a          275 c          217 g          205 t
ORIGIN      
        1 aacgccaatc ttgtaggccg ggacacggaa caccgaacag acaatttcag ccgtcatttt
       61 cagttgttcc accgtctgcg catccaccgg tgaaaacgtc gtggggctgt attttgcccc
      121 gttgctcagt atggccgtct tcccggcatt ttcgcccgta tatccgctgt cccagttccc
      181 cttcagtttt ttcgcgtttt cttccgtaat actgccgggg acctcaatca cgcctgatgg
      241 tcgcccgcca tttctgaaaa aatacgtcga atttgcctga atatgatgcc cctgcatggc
      301 ggccagtcct gcggcataca ccggcggcag ccccacaagc ggatgaaaaa aacagttaaa
      361 acggtcgtgg atcacctccc gggcaggcac cgtcaccgac tctgtgatcc cacagttccg
      421 gtccggcgtg atgcggtaga acacctcgcc gtcatccgcc accagaggtt caacccggtt
      481 ccagtccaga atacgcagtt ctttgatctg cccccggggg gtgcggattt tcagcaccac
      541 cgtattgccg tgacgcaatt tggaattcag ccacagttca aaaaactgga tgcgattctg
      601 ctgcgcatta ggacgacgac agagacgggc agtatctccc tgccgctttt cccggcgtat
      661 tccctgtaca tcagtctgca tcaggcgaag ccgcattttg gcaatatcct gggatatcag
      721 cgaaatgcat gaaaacaccg cgtgaaagga caaaacggtt tccggatcgg ctttcacccc
      781 ctgctgccag gcaccggcaa aaggctcagc caccgcctga aacagggacc gccagcccac
      841 ttctcttacg tcacgtcctg atttctggtt ttttcgggtt cgccg
//
LOCUS       DAZFKY010000474          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFKY010000474 DAZFKY010000000
VERSION     DAZFKY010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(44..780)
                     /locus_tag="ACHKCL_005164"
                     /pseudo
     CDS             complement(44..780)
                     /locus_tag="ACHKCL_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          224 a          188 c          201 g          263 t
ORIGIN      
        1 gttattttac ttttccctta tcgctgtcga ttctcattat cattcatggc aaaaaaacag
       61 caaaagaggc cctggtaatg actcctgtca gtagtacatg tgttttcgat aaatgaacca
      121 atggcttttc gtgaatttcc agggagcgta aaaagcaaat cgttttccgg gcaggtcgtt
      181 tgatgtgagt acgaagtgtc agattgttgc gctcaatgcg ccgggtaaat atcttgccga
      241 caagatgctt atcctgtggc atttctctgg tataactgct ccggttgtct ctggttatca
      301 tgcctgcgga gaatggcttc aggaattccg gcaactcacg gcatgtttca tcagtgcgag
      361 gaccaaaagt gtaagccagc acaccgtcag ctttggtctt atacgcgtac cagtgccact
      421 gctgacgagc tttgttttcg acaaaactcc attgttcatc aagttcacag ataagtgcca
      481 catcgacact ggcggctgga gatatggtta ctttacgtgg tgagcgtttt ttaaagtgag
      541 aatgacggtg ttgatatcca ccttcagcgt tctggagcta tcgcgaaccc cggctccgtt
      601 atgaaccatt tcaacaattt gctctttaat gcctggtttt cgggtttcgt agatgtaatt
      661 cagttgaaat acacgtttgc aggattagca ctggaaacgc tcatgatctg aggtgctgtg
      721 atcataacgg tacactttat aggaattaca gcggggagaa tatactgtaa ctgttgccat
      781 atggtctcca gataccaata gaatacaaca ttaatctatc gtcagaaggc atcaccgggg
      841 ccttctgaca taatctgtta atacgtgtac tattac
//
LOCUS       DAZFKY010000475          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFKY010000475 DAZFKY010000000
VERSION     DAZFKY010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHKCL_005165"
     CDS             complement(<1..176)
                     /locus_tag="ACHKCL_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765916.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHL"
     gene            complement(173..>874)
                     /locus_tag="ACHKCL_005166"
     CDS             complement(173..>874)
                     /locus_tag="ACHKCL_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5765917.1"
                     /translation="AEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQ
                     NMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGT
                     AIQAAAANFHFAIGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAE
                     GGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEL
                     RLQLRDGGMLSGSGR"
BASE COUNT          180 a          325 c          192 g          177 t
ORIGIN      
        1 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
       61 gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc
      121 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaaggt tttcatcgcc
      181 cgctccccga taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct
      241 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttggtgccgt
      301 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      361 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      421 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcgat
      481 gaacaattcc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg atcgcgaaat
      541 ggaagttcgc cgccgcagcc tgaatggccg tccccgagga agcagatgca ccaccaccga
      601 aagcaccgcc aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      661 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      721 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt
      781 taacctgcga aaaactgtcc gtcgcacttt ccgcccactc gccccagccg gacttcagcc
      841 cggccatcca gcttccacga agctgctcct ccgc
//
LOCUS       DAZFKY010000476          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFKY010000476 DAZFKY010000000
VERSION     DAZFKY010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..532
                     /locus_tag="ACHKCL_005167"
     CDS             2..532
                     /locus_tag="ACHKCL_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5765918.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
     gene            complement(576..>874)
                     /locus_tag="ACHKCL_005168"
     CDS             complement(576..>874)
                     /locus_tag="ACHKCL_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765919.1"
                     /translation="GKGLTIPHHAGIVVHFAVDAGENLVLRQNTTIGQIDGDMPSSRV
                     KIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTYITKKSGVVLYK"
BASE COUNT          253 a          184 c          198 g          239 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atggagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcgggtat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggctttat gtactctatt tatacaatac aacaccactc
      601 tttttagtta tatatgtgca gtttgatggt atatctttat ttataaaaga cattgcacct
      661 atttttacat tatccccaat tttacgtgat aatccaatga tgcaacaatt agctccgata
      721 tcaacgttac taccaatttt tactcttgaa ctaggcatat caccatctat ctgtccaatg
      781 gtagtattct gtcgtaatac cagattttca cccgcatcaa cagcaaaatg aacaacaatt
      841 ccagcatgat ggggaattgt caaccctttt ccaa
//
LOCUS       DAZFKY010000477          873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFKY010000477 DAZFKY010000000
VERSION     DAZFKY010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..514
                     /locus_tag="ACHKCL_005169"
     CDS             <1..514
                     /locus_tag="ACHKCL_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO5765920.1"
                     /translation="ELSARKGEGEGEGEGEGEGEGEGEGEGEPKSTVSVFYLQPEEVN
                     TLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHATAQYRLYVELQEQG
                     ETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAYKLKASKQPLDADR
                     LIEELTKEQK"
BASE COUNT          321 a          138 c          202 g          212 t
ORIGIN      
        1 ggagctttct gctagaaaag gtgaaggtga aggtgaaggt gaaggtgaag gtgaaggtga
       61 aggtgaaggt gaaggtgaag gtgaaccaaa atcaacagta agcgtctttt atttgcagcc
      121 agaagaagta aatactttat cacaccaggc aaaacgaggc gatggcgagg cgggttttag
      181 gttgttcttg tactacaagt tgtccaatta tgatgaagaa aaaagtagtc agtggttaaa
      241 aatagcggct aataatggtc atgcgacagc tcagtatcgg ttatatgtag agttacaaga
      301 acaaggtgaa accaaggaag ctaatgaatg gttacaaaaa attaaattgg ctgccagtaa
      361 taatgacagt tcagcaatat ggttcttggt gaattgttat aaaaaccagg gaaataaaaa
      421 agaagcattg caatgggcat ataaactcaa agcgagtaaa cagccgcttg atgcagatcg
      481 actaattgaa gaattaacga aagaacaaaa gtagctttaa acaggtaaca gaaaaatgga
      541 gagtagaaaa actttaccag tcgctacaac acctacacac aaaattacgt ttacgacgac
      601 tgcaacaagc tcatcaaagc gcaagttgaa accaaaactg gcatcttcgt cgccggttat
      661 cattacgatg tacttggcag atgcatcaaa agtcgcaaga gaaaagatga aaatatagct
      721 ttttgtatca ttaagtatgt ggccagcaca tgactcggca tagatcttat gactaagatt
      781 ctacacccca aacctaagta tgggccaaag aagccgtttg gttcattttt tatatctcca
      841 gcattttagt ggatggagct tatgaaaata ata
//
LOCUS       DAZFKY010000478          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFKY010000478 DAZFKY010000000
VERSION     DAZFKY010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            83..>869
                     /locus_tag="ACHKCL_005170"
     CDS             83..>869
                     /locus_tag="ACHKCL_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5765921.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLS"
BASE COUNT          287 a          168 c          165 g          249 t
ORIGIN      
        1 aatatatatt tatgccgtaa ataccaatat acttagcaaa ctatgtgatt tccatttcga
       61 ttgatttagt gtttattgac gtatgtactg gattattaac gataatatcg agttctggcc
      121 tgagcaccga aaattaatat cggtacataa cgccgatctt aacgtcgttc tgacaacgcc
      181 agccagtcgt tgtttatcac tgctacttga agcttttcct gatgtggttg cacaacaaga
      241 ttttttcacc agagtctggg aagaagaagg tatgcgtgtg cctactaaca cgttatatca
      301 gaacatatcc attatcagac gcggatttcg cgctgttggt gatactaccc actcgcttat
      361 tgcaacagtg ccgagaagag gattcaagat ccataatgac attaacatac aaaatcatgt
      421 aataaactca tcaacagacg cacatacaca caatgcccca cctgccataa aagttaatgc
      481 gggatacaaa gagagcattg gtggcgcaaa gaatttcaat aacaaaatcc tcaaacatat
      541 aaaaagttat ctaattatgt tgagcgcatt tgtcataggc gcatattctg catattggct
      601 atggaataat aatcaaccga agccattttt caaggattac aagactgttg cggaaattaa
      661 tggctgccat tttaatgtaa cagaagatac aattgatggg ttgaaagagt tcgataaata
      721 taaaacacgg atactggatt cggggattaa ctgcaaaaaa tatccgtggt tatacttccc
      781 tcttgcaaaa tcctcaccgg ggatgattgt tatggcatgc aataaaaact ataaccaaca
      841 tgaagtggca ggctgtttaa ccctgtctt
//
LOCUS       DAZFKY010000479          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFKY010000479 DAZFKY010000000
VERSION     DAZFKY010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..566
                     /gene="espL2"
                     /locus_tag="ACHKCL_005171"
     CDS             <1..566
                     /gene="espL2"
                     /locus_tag="ACHKCL_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO5765922.1"
                     /translation="TPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDG
                     APGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHV
                     VKLYGKLFKKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFN
                     ICPEKVAEYLSEFSGKHFLDVYNYYSN"
BASE COUNT          303 a          135 c          165 g          261 t
ORIGIN      
        1 atactcctgg attattttct gcatttcaaa atggtcatgc agacgcaata aaggcatatt
       61 gtggcgtact tggcaatagt aatttgaaaa gaggtgagat aatcaggatg cttgaggcga
      121 gaaattatga tggtgcacca ggattacttc ttgcgtatca aaatggagat attaatacga
      181 ttcagtcttt ttttgactca ctaataatgt tagatatatc aaaggatttt atcgaagagt
      241 tattgactgc aaaacattat gattttacgg ggttatcatt agcgatcagt catagacatg
      301 atcatgttgt caaattatac ggaaagttat ttaaaaaact tgataccagt ccatataaaa
      361 tgtcaattat cctagcatta gcaattgatt gcgagagaaa taatgcaaac ataattattg
      421 atagtgagta caagtcaaat aaggcagtaa aggaatatgt agaaatttta aaagaattca
      481 atatatgccc agaaaaggta gcagaatacc taagcgagtt cagcggaaaa catttcctcg
      541 atgtatataa ttattattcc aattgaaaaa aatataaagt atctatcacg gcactctcag
      601 ttgagtatca caggcacatg tattttagcc ccctgagtct acagacaacc aatattactt
      661 aaataagtga taatcttaat gctgtttttt agaacagatg attgatattt ttgggccgga
      721 gaaacgcaga caggctttgt tgaataaatc gagcttttgc tgagtttaag gatcagatca
      781 ctcattttcc tgcaaaagct cattgggcga aacaatctga ctcagagaac ccggattaaa
      841 cgtctggccc gcagaatcat ctat
//
LOCUS       DAZFKY010000480          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFKY010000480 DAZFKY010000000
VERSION     DAZFKY010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..453
                     /locus_tag="ACHKCL_005172"
     CDS             <1..453
                     /locus_tag="ACHKCL_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5765923.1"
                     /translation="NLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEAD
                     PEQELSDRWVVEQMSQLTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGY
                     TGGAVADEFDNPTTDIRKDRCSKCMRGCEMRSMVANFGGFLSINKLSQ"
     gene            464..>863
                     /locus_tag="ACHKCL_005173"
     CDS             464..>863
                     /locus_tag="ACHKCL_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5765924.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          159 a          212 c          300 g          192 t
ORIGIN      
        1 aatctgttcg gtcttgtcac cgggatggcg gaggatttgc agagcctggt gggtgccacg
       61 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaatttcgt tgcgggcaat
      121 ccggaggcgg acccggagca ggagctgagt gaccgctggg tggtggagca gatgtcgcag
      181 ctgacagcca tgacagcctc gtttgtgctg gcaacaccga ccgagacgga cggagcgctg
      241 tttcccggtc gcattatgct ggcgaatacc tgtatgtgga cctaccgctc tgatgagtgt
      301 ggttacacgg gcggggcagt ggcggatgag ttcgacaacc ccaccacgga tatccgtaag
      361 gacagatgca gcaagtgcat gcgcgggtgt gagatgcgca gcatggtggc taattttggc
      421 ggtttccttt ccattaataa actttcgcag taaatcccgg tttatgacac agactgaatc
      481 agcgattctg gcgcatgccc ggcggtgtgc gcctgcggag tcgtgcggct tcgtgataag
      541 cacgccggag ggggagtggt atatcccttg tgtgaatatt tctgcagagc cggaggcgta
      601 ttttcgtatc gcaccggaag actggctgcg ggcagagatg cagggggaga ttgtggcact
      661 ggtccacagt catcccggtg ggctgccctg gctgagcgag gctgaccggc ggctgcagat
      721 aaaaagcgca ctgccctggt ggctggtctg ccggggtgac attcacaaat tccgctgtgt
      781 gccacatctg acaggacggc gctttgagca cggggtgacg gactgttaca cgctgttccg
      841 ggatgcttat catctggcgg gga
//
LOCUS       DAZFKY010000481          859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFKY010000481 DAZFKY010000000
VERSION     DAZFKY010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..183
                     /locus_tag="ACHKCL_005174"
     CDS             <1..183
                     /locus_tag="ACHKCL_005174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765925.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="ACHKCL_005175"
     CDS             187..393
                     /locus_tag="ACHKCL_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5765926.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="ACHKCL_005176"
     CDS             458..682
                     /locus_tag="ACHKCL_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765927.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            643..>859
                     /locus_tag="ACHKCL_005177"
     CDS             643..>859
                     /locus_tag="ACHKCL_005177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765928.1"
                     /translation="MPHGIRCITASVLSDLIIHARYCRRIPALTETAARPGESSARVC
                     GDNQKRYTPGFTALTERGVVPSWSLVRC"
BASE COUNT          204 a          215 c          241 g          199 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcg
//
LOCUS       DAZFKY010000482          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFKY010000482 DAZFKY010000000
VERSION     DAZFKY010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>849
                     /locus_tag="ACHKCL_005178"
     CDS             <1..>849
                     /locus_tag="ACHKCL_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5765929.1"
                     /translation="QLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETF
                     DEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQEITST
                     SRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDT
                     IKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRR
                     SYERQG"
BASE COUNT          272 a          195 c          224 g          158 t
ORIGIN      
        1 atcagttgct cgccgcatca cgtggtgaat ttgttgcagg gattagcgac ccgaatgatc
       61 cgaaatgggt gaaggggatt gaaacccgcg attctgtgaa ccagaaccag caagaaacgg
      121 aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa
      181 cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa agtctgcacc gcctgcggtc
      241 agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg
      301 aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag gaaatggaag
      361 gcgctgaaca tccacacaag gagaacactg acggcaatca gcatcacgat agcgataatg
      421 aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa atcacatcca
      481 ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg
      541 ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg
      601 aatttagtaa gactatcgat ctggaaactg ctggcggggt cattgatcgg gacaccatta
      661 aatggtggct taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt
      721 tagatgatgc actattacaa ttgcgggaat ttatcgacga aaactccggt gaattttttg
      781 ttcaggtctg gggaaatgga gccaacttcg acaacacgat tttgcgccgt tcatacgaac
      841 ggcagggga
//
LOCUS       DAZFKY010000483          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFKY010000483 DAZFKY010000000
VERSION     DAZFKY010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            107..823
                     /locus_tag="ACHKCL_005179"
     CDS             107..823
                     /locus_tag="ACHKCL_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5765930.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          281 a          143 c          174 g          244 t
ORIGIN      
        1 ttatttctaa tagacgagat ttttcctgtt atgatataat atgccgaatt aacacatgtt
       61 aacgatttac cagtaatgta aataaatttt cgaggagatc attcctgtgg gacgtaaatg
      121 ggccaatatt gttgctaaaa aaacggctaa agacggtgca acgtctaaaa tttatgcaaa
      181 attcggtgta gaaatctatg ctgccgctaa acaaggtgaa cccgatccag aattaaacac
      241 atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc acgttattga
      301 taaagcaatt gataaagcca aaggcggcgg agatgaaaca ttcgtgcagg gccgttatga
      361 aggctttggt cctaatggct caatgattat cgccgagaca ttgacttcaa atgttaaccg
      421 tacgattgct aacgttcgca caattttcaa taaaaaaggc ggcaatatcg gagcggcagg
      481 ttctgtcagc tatatgtttg acaatactgg tgtgattgta tttaaaggat cagaccctga
      541 ccatattttt gaaattttac ttgaagctga agttgatgtt cgtgatgtga ctgaagaaga
      601 aggtaacatt gttatttata ccgaacctac tgaccttcat aaaggaatcg cggctctaaa
      661 agcagctgga atcagtgagt tctcaacaac agaattagaa atgattgctc aatctgaagt
      721 tgagctttca ccagaagatt tagaaatctt tgaagggctt gttgatgccc ttgaagatga
      781 cgacgatgta caaaaagttt atcataacgt cgcaaatctc taattatatt ttaaagaaat
      841 ct
//
LOCUS       DAZFKY010000484          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFKY010000484 DAZFKY010000000
VERSION     DAZFKY010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..288
                     /locus_tag="ACHKCL_005180"
     CDS             <1..288
                     /locus_tag="ACHKCL_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5765931.1"
                     /translation="YLDNMEATGFYRISLPSAQPGDILLCCFGASVPNHAAIYCGNGE
                     LLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHASAFTGIYNDLAAASACM"
     gene            285..>841
                     /locus_tag="ACHKCL_005181"
     CDS             285..>841
                     /locus_tag="ACHKCL_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5765932.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKV"
BASE COUNT          175 a          230 c          263 g          173 t
ORIGIN      
        1 tacctggaca atatggaggc gactggtttt tacaggattt ccctgccttc cgcacagcct
       61 ggcgatattc tgctgtgctg ctttggtgct tcggtaccga accatgccgc catttactgc
      121 ggcaacggtg agctgcttca ccatctgcct gaacaactga gtaaacggga gaggtattcc
      181 gaaaaatggc aacgacgaac gcattcagcc tggcgtcacc gccactggca cgcatctgcc
      241 ttcacgggga tttacaacga tttggccgcc gcctcagcct gtatgtgaac acggcagcgg
      301 aagccatccg ggcgctgtcg ttacaggtgc ccggattccg ccgtcagatg aacgaaggct
      361 ggtaccagat acgtattgcc ggtgatgaca cggcaccgga ggcggtgtac gcccgtcttc
      421 acgaacagct gggtgaggga acggtcatcc acattgtgcc gcgactggcc ggggccggaa
      481 agggtggact gcagattgtg ctgggggcgg cagccatcgt gggctctttc ttcacggccg
      541 gagcatcgat ggcagcctgg ggtgcagcgc tgagtgccgg cggtttttct gccaccacga
      601 tgctgttttc actgggtgcc agcatgatac tgggtggtgt ggcacagatg ctggccccga
      661 agccaaaaac accggaatac agggcaacgg ataacggtaa acagaacacg tacttttcgt
      721 cgctggacaa catgattgcc cagggtaacc cgatgccggt gccttacggg gaaatgctgg
      781 ttggctcccg ccgtatatcc caggacatca gtacccgtga tgaaggcggg ggcggaaagg
      841 t
//
LOCUS       DAZFKY010000485          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFKY010000485 DAZFKY010000000
VERSION     DAZFKY010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(194..721)
                     /gene="sodC"
                     /locus_tag="ACHKCL_005182"
     CDS             complement(194..721)
                     /gene="sodC"
                     /locus_tag="ACHKCL_005182"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5765933.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHPGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          206 a          199 c          219 g          214 t
ORIGIN      
        1 taaaagcgaa aaaatcccgc agtgctcacg gacaggaact gcgggagcgt tacgaagatt
       61 gagtgtaagg aattattctt atgtcacgac aaaaacatta actcagagag ggaggatgtg
      121 ccgttctttt cagggagaaa ggatttatcg tcctgaggaa taaaggtaag gggcccgccc
      181 cttacctgac tgattattga atgatgccgc aggccattct cgcaccacca ccgcccaggg
      241 gctccggatg gtcatgatgg ttatcaccgc cagcatgaag catgagagaa cgccctttaa
      301 tctcttttaa tgagttcagt ctcggggcca ggaccgggta gttcgctttt ccgtcatgcg
      361 tcacgaacag cgcagggagg tcgcccgggt gtccatccgg agaccagggg ccaagatgtt
      421 tgccggtgtt tttcgggtca aagtgaccgc cagccgataa tgctgcgacc ggttttccgt
      481 ctttcagtgc cggggcgcaa tttccttttt cgtgcacatg aaaaccatga atgccttcag
      541 acagagagtg aagggctggt gtgaacagca gaccgtaggg ggtctcctga atggttattt
      601 ttccaatgct gacttctttt ccgtcagcac tgacaaggtt cattgggact tcctgttctg
      661 ctgcgtatcc gcatgatgct gctgtcagca tggcaatggc agcaatgatt ttacatttca
      721 taaaaccctc attaattccg ttaacagact gagcttgctg gttacagggt aacaaacagc
      781 gttctgatga tatcgcgcaa tagctgtgca atatcctatc actgcgatta ataatacc
//
LOCUS       DAZFKY010000486          828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFKY010000486 DAZFKY010000000
VERSION     DAZFKY010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>828
                     /locus_tag="ACHKCL_005183"
     CDS             <1..>828
                     /locus_tag="ACHKCL_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO5765934.1"
                     /translation="TTIIKKTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSD
                     VPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKC
                     LGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDDFFLRKTQRIITM
                     SNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSLALQQISSFWRVT
                     DPNFGHCDFHSLAQALTFIKNITSNRNFSSLYGSGIVKIYFSESLNNWKYIKLPLVQ"
BASE COUNT          294 a          140 c          133 g          261 t
ORIGIN      
        1 taacaacaat aataaaaaaa acagaatatt atgggcatca gttaacaaat gctatgactg
       61 ttgcttcagt ggttaaacca tatgacttta gcaatcttgg tgccataaat agtatagata
      121 aatccgtttc tgatgtgcct gcattgcata caattgttga acaagcaaaa tataatttac
      181 tttcatggaa tgatttttat aacactcatg cgtcaatatg ggatacaata gcaagacagc
      241 ataaaagtac aaatatcgaa tttcacccac aaagtcttct ttttgataga gattcgaaag
      301 gaaagtgcct tggtttatct cttctgtatc ttgatacagg ggggtacgga ggcggatatc
      361 aaaaactgag gcacaatatt gacactgcca gcacattata tcaaacaaaa tacaatgaca
      421 atctaaaatt atctaaccgt gatgatttct ttttaagaaa aacacagcgt atcattacaa
      481 tgtccaatga gttaggtaat aacaggttaa aaaatgcaca gttagaggtt ctggaattga
      541 aagaccctat actaactgaa ggcatattat accagcgtcg tatatcatct cttttaataa
      601 caacagaata tcattcactt gcattacaac aaatatcttc tttttggaga gtaactgatc
      661 ctaattttgg tcattgtgac tttcattcct tagcacaggc tttaacgttt ataaaaaata
      721 taacgagcaa tcgtaacttt tcatctttat atggaagtgg cattgttaaa atttacttca
      781 gtgaatctct taataattgg aagtatatta agctccccct tgttcaga
//
LOCUS       DAZFKY010000487          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFKY010000487 DAZFKY010000000
VERSION     DAZFKY010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..669
                     /gene="yqeH"
                     /locus_tag="ACHKCL_005184"
     CDS             37..669
                     /gene="yqeH"
                     /locus_tag="ACHKCL_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5765935.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          269 a          143 c          160 g          255 t
ORIGIN      
        1 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
       61 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
      121 ttgcagtact gcgcaaactc gtgtttttgt atgtggaccg aagaaagttc atattttaat
      181 cgtggtgtgg tagaaggtat cttaacaaaa aaccacaatg cgagattaag cggatatatt
      241 tttgtcgatt tttcagtaag ttttttacgt ctattccttg aaaaagactg gattgactat
      301 cttgcaagta ctgacatggg gattgtttta gtcagcgacc gcaatatgca atcgttagct
      361 aattactggc gaaaacacaa ctctgcaata tctgctgtca tatataatga tgatggtctt
      421 gatgttgcca atgaaaagat cagacaacta tttattggtc gttatttatc atttaccaga
      481 ggtaacacat taactcagat ggaattcacc ataatggggt atatggtttc tggttataat
      541 ccatatcaga ttgctgaagt tctggatatg gatatccgta gcatctacgc gtacaagcaa
      601 cgaatcgaaa agagaatggg tggtaaaata aacgaattat ttattcgttc acattcggtc
      661 caacattgat actcaacaat ctgccagaat catactctgt ttatacgggc atttattgaa
      721 atgcccgtat tttataaata ggccgtcccc tcaagagtaa acaccatagt atttaaaacc
      781 atcacacgaa aaattcagaa gcattacgaa atatgaactt acaaaca
//
LOCUS       DAZFKY010000488          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFKY010000488 DAZFKY010000000
VERSION     DAZFKY010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHKCL_005185"
     CDS             complement(<1..143)
                     /locus_tag="ACHKCL_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5765936.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHKCL_005186"
     CDS             complement(91..657)
                     /locus_tag="ACHKCL_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5765937.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          217 a          194 c          131 g          267 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaattttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acactacccc cgataattc
//
LOCUS       DAZFKY010000489          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFKY010000489 DAZFKY010000000
VERSION     DAZFKY010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(55..>806)
                     /locus_tag="ACHKCL_005187"
     CDS             complement(55..>806)
                     /locus_tag="ACHKCL_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765938.1"
                     /translation="EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSV
                     ADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTG
                     KGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAIT
                     TKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          214 a          189 c          154 g          249 t
ORIGIN      
        1 aaataaaaga gaaataaaaa aatagtaaca agcatgcttt catacatcta caacctatct
       61 tatatcactg ggtttttctg gcgtaccttg catattagaa atggggtgcc cagatggccc
      121 atatgttatc caattaccaa ggtcttttgt tgttatagcc attagatcct tatttgaatt
      181 aaggttcgtt ggtgctgtta gcactgtatc catttttaac ttaccattag ttcctcgccc
      241 ccaccacgtc catagcgtgt caggtcctat tacatttgta tgtaactctt tagctaattg
      301 gctagcaaat gaattttgtc ctttacctgt attacaagaa aataaccaaa ctgtcatccc
      361 atctttataa ttcccatcaa atttaattag atatgctaga tctttagctg tcatgatact
      421 gcgcgttgcg gattcaatag atgtgggggt accatgcccc ccgattgtga aaacgcctgg
      481 gatattaatc tcatcagcta cagaatggat aagatcactt tcggggtata aatttatatc
      541 aaccaaacct tgcggatcta catttatgac cggattcaac ggatactgat aaaaattcca
      601 tccccccttc agtccaatcg gatcctgagt gatatatcgc ccgtgcagcg ggtcataata
      661 gcggtggcgg ttgtaataca ggccggactc ctcatcatac tgctgccccg gcaggcggat
      721 aagctgctgc agctgatgcg ggttctcttc attcagcagg ttgccccatt catcgtattc
      781 tgcgcaccat tctgttgccc cttccg
//
LOCUS       DAZFKY010000490          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFKY010000490 DAZFKY010000000
VERSION     DAZFKY010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            146..>804
                     /gene="escD"
                     /locus_tag="ACHKCL_005188"
     CDS             146..>804
                     /gene="escD"
                     /locus_tag="ACHKCL_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5765939.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DV"
BASE COUNT          244 a          163 c          144 g          253 t
ORIGIN      
        1 cattggaaac tcacgtaatc attaattatg aatattccat taaatagtac tgtacaacag
       61 cttcctttct actctattct tagacgatgt aagttcacca tattttttct cattgttcaa
      121 ccaccaggat gaataaaatt taaaaatgtt atcctcatat aaaataaaac ttctcaatgg
      181 tgcgatgcgt aatcgggaat tacaacttcc gatgggaaac ctaacgctag gtacggaaga
      241 taacgatatt gtttactttc cccttgaaca aggcctcaat cagtttttac ttgatatacg
      301 tgaggaaggt gttttccttt tatctccggt tgaattttgg attgatgggc aacccacgcc
      361 ctatgaagca gataaaccat tacctgtcgg aaaggttatt gatatagcgg gatgttgctt
      421 catcattggt gatattgacc attccttgcc attgtctgat gtgccagaaa ggttctctgc
      481 caaaagtcga cgcaaaaaac gccttatcct ggcgagcgtg attggtgcta cttttgcgtt
      541 gtctggtgcc atcggcagtt acgttttgct ttccccaaaa gctgaacccc caacgtttac
      601 cagagctgat gtatatcagc agctaaaaga aaacaaactt catgcaatca ctctggtatg
      661 gcatgggaaa aatatcgcgc tttatgggcg ttgcgaatca accacggatt taactccatt
      721 ttttaattac ctaaaagaaa aaaatatttt ttattacaat aatatcattt gcaataacca
      781 aatcatatcc gcaattaatg atgt
//
LOCUS       DAZFKY010000491          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFKY010000491 DAZFKY010000000
VERSION     DAZFKY010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>800
                     /locus_tag="ACHKCL_005189"
     CDS             <1..>800
                     /locus_tag="ACHKCL_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5765940.1"
                     /translation="NFLDGRGIDIIERVELKKYLSVAEIDDLVRYAKQRFDRQKIINI
                     KSTNYRFIAKRTFSYRIHVFSRYLKWLCGLVHSSKGIHAKYEVDVFIESIRAHIPRNS
                     SLNMNEISEKSLNEEEIKVLFRLLEIGGIENPFHKEVQVRNRLIFTLLLNLGLRAGEL
                     LNLKIDDFDLRDNTLSVVRRHDSKEDGRSYQPLVKTGERVIPLSDELAREIFDYISNS
                     REKMTKRKKHNFLFVAHCTGKNAGEPLSISAYEKVISTLKRASPELYN"
BASE COUNT          299 a          110 c          166 g          225 t
ORIGIN      
        1 taatttcttg gatggtaggg gaattgatat aattgagaga gttgaactta aaaaatatct
       61 cagcgttgca gaaatagatg atctggtaag atatgcgaaa caaagattcg acagacaaaa
      121 aatcataaat ataaaatcga caaattatag gtttattgcg aagagaacct ttagttatag
      181 aatacacgtt ttttcacgct accttaagtg gttatgtgga ttagttcatt cgtcaaaggg
      241 gattcatgca aagtatgaag tagacgtatt tattgaaagt atcagagcac atattccaag
      301 aaacagttca ttgaatatga atgaaatatc tgaaaaatcg ttgaatgaag aagagatcaa
      361 agtgcttttc cgtctattgg aaattggcgg gattgaaaac cctttccata aggaagtaca
      421 ggtcaggaac agactaatat ttaccttgct cttaaattta ggtttaaggg ctggagagtt
      481 gctcaatctt aaaatagatg attttgatct cagagacaac actttaagtg tagtcagaag
      541 acatgattca aaagaagatg gaagatctta tcaaccatta gtaaagactg gagaaagggt
      601 tattccgtta tctgatgagt tagcccgtga aatttttgat tacataagca atagcagaga
      661 aaaaatgaca aaaaggaaaa agcataattt cttatttgtg gctcattgca ccggaaaaaa
      721 cgcaggagaa cccttaagca tttcagctta tgagaaggtg atatcaacac tgaaacgagc
      781 aagtccagag ctatataatc
//
LOCUS       DAZFKY010000492          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFKY010000492 DAZFKY010000000
VERSION     DAZFKY010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            55..>800
                     /gene="espR3"
                     /locus_tag="ACHKCL_005190"
     CDS             55..>800
                     /gene="espR3"
                     /locus_tag="ACHKCL_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFO5765941.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDSNLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKE
                     NVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISY"
BASE COUNT          262 a          125 c          168 g          245 t
ORIGIN      
        1 tattgtgttt tagaatgcgc cctgggcaat ataatttatt aaacaggagt gtaaatgttt
       61 ccattaaatg atctgtcgtt aaaaactcag tcggttcaat taaataaaat cacatcgaat
      121 actgagtcta cgataaaaca acacgagtta gtatctgatg atgcaatcat aaatgaatta
      181 tcaagtgagt tagtcagttg tcttggaaat gataagttta caccagttag tgaagacagc
      241 aacttactga atatgctgtc tgaatttaag ttattgagag agcaatgttt caggtggggt
      301 aattatactc tattgtttga aaattatggg gcttatgata agacgggatc tatcacgata
      361 gaaaaaagtc agggggaggg gactttaccc attcggcata aattagagtt tatatcgacc
      421 aatattgcag agttgctgga caagttaacc aaaattacag atgccaggct ttgcaaaggt
      481 ttcagtgact gggctagttc agtcaaagaa ggcgcatcga atgacttgaa agaaaatgtg
      541 gatagagcat tggtgagaat gtttaaatgt gttaagcttc acagtaatga acttaactta
      601 tcaagccttt ctttgggttc tgtgccgcct cttcctgagt ggattgaaat gcttagcctt
      661 gtttataatg aacttgattc aatacaggtg cccgaatcgt gcaaagaatt agaactcgat
      721 ttcaataacc ttacagaatt tccacaagta cctgatggaa ttaccctgat ctccgtaaat
      781 aataacctga tatcgtatat
//
LOCUS       DAZFKY010000493          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFKY010000493 DAZFKY010000000
VERSION     DAZFKY010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>795
                     /locus_tag="ACHKCL_005191"
     CDS             <1..>795
                     /locus_tag="ACHKCL_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765942.1"
                     /translation="FVSESDFQLSELDQLAQVESRQITSEIRGGEFVDDDFRQLVEML
                     KLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELSDASDTKGYWSTDV
                     KLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAYKSIYYSVIAKHFK
                     ADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSEIVDHGRTSTISLD
                     LPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTYD"
BASE COUNT          247 a          129 c          170 g          249 t
ORIGIN      
        1 aatttgtatc tgaaagtgat tttcaactca gtgaacttga tcagttagca caagttgaat
       61 ctcggcaaat tacttccgaa attagaggtg gggagtttgt tgacgatgat tttaggcaac
      121 ttgtagagat gcttaagctg aatatgattt gcacatggga tcttcggtct agcgtgtatt
      181 acttaacaat gaaaatgctt ggtcagcccg gtactcctga atatgcgaaa tatagtgaac
      241 taagtgcatc gatttttaat gaattatcag atgcttctga tacgaaaggg tactggtcaa
      301 ccgatgtgaa gctagtgagt tcatctggac atgaatatac tgagcgtgaa tttgaactag
      361 aaagggaaaa atctactcgc ggtaaaggag gtatgactcg ttcacttgaa atgtttatcg
      421 catcgctaaa ttggttggca tataaatcta tttattacag cgtcattgct aagcatttta
      481 aagcagactc atttattcat ccaataaggc atgcatatca attgcattgg atgaaaaaaa
      541 caggtgcttt tggtcatgat tatactgcaa ggttggtgca atcgttgtct gataaaatca
      601 gtactgcaag atctgaaatt gtagatcatg gacgcacatc tactatttca ttagatttac
      661 caatattttc tgcatggcta gcaaacgaaa caggaaatgt ctctcaagtt atcaattcag
      721 ctttagagct tagaacacat gatcattttc gtacgtgtcg agatgtaatc cgagagattc
      781 atgtgaccta cgatg
//
LOCUS       DAZFKY010000494          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFKY010000494 DAZFKY010000000
VERSION     DAZFKY010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            176..251
                     /locus_tag="ACHKCL_005192"
     tRNA            176..251
                     /locus_tag="ACHKCL_005192"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Ile,seq:cat)
BASE COUNT          228 a          149 c          180 g          236 t
ORIGIN      
        1 taacatttgc ttgttgactg cgagtatcga ctttgcggaa tggaggtggt gtcgggtttt
       61 atgtcgcatg tattgacgac ccaggatgga tgagaacgca taaaaatctg tgatttacat
      121 cataagatca tcatgtttat tgcgtgggtt ataagtattg ttattgcata cttaaggccc
      181 tttagctcag tggttagagc aggcgactca taatcgtccg gtcgctggtt caagtccagc
      241 aagggccacc aaatcatcat tcatgtatca ggaagagtta agggtacaag ggttactcga
      301 tggcggtcta tatttcacga ttaacgccat ttcaaggaag aagtgcggtg ggtggtttcg
      361 aatgagggct ggtgttttgg tacgtggcac gccactgcaa tgtatttatc tatcagtact
      421 cattcaaaag tatttggaaa aaatgcctgc tatgccaagc gggcaaattg cttacatgtg
      481 gaattaagta attaacgata aagccagcgt attatcattt attataccca ttcatttaat
      541 caggttaatt actcaaatca ctgaactatt ctctgttcgc tcactgatga taagggagta
      601 ccacaaccaa tgtatgtaga acaatgccgt agagtaagga tgaaatgtct ctgccacgcc
      661 ttaaacatct actactgact agaacacccg ccgcaggtag atgagttttg tgagcgttac
      721 aggagcgtgg ctatgctcat taatcaacgc tatttagata taaatttcta acactcgaaa
      781 aacgattttc act
//
LOCUS       DAZFKY010000495          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFKY010000495 DAZFKY010000000
VERSION     DAZFKY010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..272)
                     /locus_tag="ACHKCL_005193"
     CDS             complement(<1..272)
                     /locus_tag="ACHKCL_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO5765943.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALR"
     gene            complement(442..518)
                     /locus_tag="ACHKCL_005194"
     tRNA            complement(442..518)
                     /locus_tag="ACHKCL_005194"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(619..694)
                     /locus_tag="ACHKCL_005195"
     tRNA            complement(619..694)
                     /locus_tag="ACHKCL_005195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Ile,seq:cat)
BASE COUNT          186 a          213 c          203 g          188 t
ORIGIN      
        1 cgtaacgcgg cacgacgacc aattttaaaa ttggtatcca gctgaccgat aatagttgcg
       61 ctaatcgcat taatttccga ggtaaacgca gacaacgcag gctgattacg taagacctgt
      121 tcgatcttcg ctttctcgga tgcctcgcat tcaccatctg cataggccac caggtatgca
      181 gcgttaatca ccgcctgtgc cagatcgcgt ttctcaaact ttttaatttc cgctgccgct
      241 ctgcgggttt tctttttgaa gattccaaac atcgtgacgt tcctttgggt gggtgagcca
      301 acgcccggga gcgatctgcc cacagagaaa gtcacactga ccactccgta agctcacccc
      361 cgaaaggctc tgtggttgat atgcgccggg cgtggcgcag atacaaaaaa ggcccgccga
      421 agcgagcctg gaaaataagt gtggcgcgtt gtagtggagt cgaaccactg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcag aataccgata atggaccgcc
      541 atcggggacc cgcccccgca ccaacaaccc tgttatggtg tcgtctgctc ttcctgataa
      601 gctaatggcg gtttgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat
      661 gagtcgctcg ctctcaccac tgagctaaag ggccgggagc agaataataa cggtccgtaa
      721 ttaattccgc aataaaaaac ccgctcaatg gcgggttctg gtaaagttca tgcgtttggt
      781 tcgcctcgcg
//
LOCUS       DAZFKY010000496          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFKY010000496 DAZFKY010000000
VERSION     DAZFKY010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            127..533
                     /gene="ipbA"
                     /locus_tag="ACHKCL_005196"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             127..533
                     /gene="ipbA"
                     /locus_tag="ACHKCL_005196"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          231 a          165 c          157 g          233 t
ORIGIN      
        1 acgttctgat tgcaggcata cttatctggg tgtgcctctc ttttttacaa ctgtacacgg
       61 tggcgtatga cgagaaaata tctcacacag gatgaagtct acaggctgat ggatgcagcc
      121 cagagcatgt cctttcctga aagaaatcgc tgtctaatta tgatggcttt cattcacggt
      181 tttagggcca gtgaacttct tgatttacgt ttatccgata ttgatgcatc aggaaaacaa
      241 cttaatattc ggcggattaa aaatggtttc tcgacaacac atcctcttct tcctgatgaa
      301 tataatttga tcaagttgtg gcttaagcag aggaagttaa ttgaaaacgg agttgaagga
      361 gactggcttt ttctctcccg gaaacgccgc cctatcagca gacaacattt ttttctatca
      421 ttcgtgaggc tggaagacgt gcaggattag ctgtaaaagc acatcctcat atgttacgtc
      481 atgcctgtgg ttttgctctg gctgacaatg gtgtcgatac ccgactactt caggattatc
      541 tgggtcatcg aaatatccag catactgtca gatacacagc cagtaatgct gctcgattta
      601 aaggggtgtg gaaaaaaaag cctcgttaaa acagtacctt ctgattcaaa atgtcagatt
      661 cctacgtttt cctcaattct tccataaaaa aacagcccat tcaattcaat aaaacacata
      721 acatgttgat atttctcgct aaagtaaaat aaccccatga tttgaaatga ttaaccgcca
      781 aggtca
//
LOCUS       DAZFKY010000497          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFKY010000497 DAZFKY010000000
VERSION     DAZFKY010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..683
                     /locus_tag="ACHKCL_005197"
     CDS             <1..683
                     /locus_tag="ACHKCL_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5765944.1"
                     /translation="DDVDIDLVVGFYKNLEDDNDDVLEKRSSDDAGWTVICNDRVVLY
                     CDKTHLTGWGFANVPRFHTQFIAISGVVRFTSKNPEKLPITTTKRGVDLSSTLYNDVR
                     NKMIEGMMHFIRFTNQWKGEHLEEGKKLLKSAQSHEAQSLFEITPQSTPEDKKNNWSN
                     PNRNKNEWRYTPKLPTPVKKSSTVRIIFTREKEDVKILSKYFFGHEDASASDVGMQCF
                     DTVIEEVK"
     gene            683..>774
                     /locus_tag="ACHKCL_005198"
     CDS             683..>774
                     /locus_tag="ACHKCL_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFO5765945.1"
                     /translation="MSGQYIPYHLRHNKSIDREIFLESLNLLSK"
BASE COUNT          271 a          128 c          153 g          222 t
ORIGIN      
        1 tagacgatgt tgatatcgat ttagttgttg gcttctataa aaatttagaa gatgacaatg
       61 atgatgtttt agaaaaaagg agttctgatg atgctggatg gactgtcatt tgcaatgacc
      121 gcgttgttct atattgtgat aagacacatc tcacaggttg gggctttgct aatgtaccaa
      181 ggtttcatac ccaatttata gcaatttctg gggttgttcg ctttaccagt aaaaacccag
      241 aaaaattacc aataacaaca acgaaacgag gtgtcgatct tagttctaca ttgtacaatg
      301 atgtacgaaa taaaatgatt gaagggatga tgcattttat tcgattcacc aatcagtgga
      361 aaggtgagca tcttgaagaa gggaaaaaat tacttaagtc agctcagtca catgaagctc
      421 aatctttatt tgaaattacc ccacaatcaa ctccagaaga taaaaaaaat aactggtcca
      481 accctaatag aaacaaaaat gagtggcgat atacacctaa gctgcctacc ccagtaaaaa
      541 aaagctctac tgtacgaata atcttcacca gagagaaaga agatgttaaa attttatcta
      601 aatatttctt tggacatgaa gatgctagcg cttctgatgt ggggatgcaa tgctttgata
      661 cggtgattga agaggtgaag tgatgtctgg acaatacatt ccctatcact taaggcataa
      721 taagtctatt gatagagaga tttttctcga aagcctaaac ctcttaagca aaag
//
LOCUS       DAZFKY010000498          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFKY010000498 DAZFKY010000000
VERSION     DAZFKY010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            147..716
                     /locus_tag="ACHKCL_005199"
     CDS             147..716
                     /locus_tag="ACHKCL_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5765946.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          217 a          162 c          200 g          191 t
ORIGIN      
        1 gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt tcaggatgac
       61 ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc tgtgagacaa
      121 ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt cacggcaact
      181 ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct gtcgttgcgg
      241 ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta
      301 taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg tccatgccac
      361 ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa cccgaaatcc
      421 gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag tactggacga
      481 agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac caggcttgtg
      541 ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga
      601 aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa gcgaatatgc
      661 tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg gattgatggg
      721 gtggctaatg atatcagata aactcataac gctggtgaag agcctctgtg
//
LOCUS       DAZFKY010000499          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFKY010000499 DAZFKY010000000
VERSION     DAZFKY010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..102)
                     /gene="rrf"
                     /locus_tag="ACHKCL_005200"
     rRNA            complement(<1..102)
                     /gene="rrf"
                     /locus_tag="ACHKCL_005200"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(197..>769)
                     /locus_tag="ACHKCL_005201"
     rRNA            complement(197..>769)
                     /locus_tag="ACHKCL_005201"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          161 a          244 c          179 g          185 t
ORIGIN      
        1 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
       61 cggcatgggg tcaggtggga ccaccgcgct aaggccgcca ggcaaattct gttttatcag
      121 accgcttctg cgttctgatt taatctgtat caggccgaaa atcttctctc atccgccaaa
      181 acaccttcgg cgttgtaagg ttaagcctca cggttcatta gtaccggtta gctcaacgca
      241 tcgctgcgct tacacacccg gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc
      301 ttaaagggtc agggagaact catctcgggg caagtttcgt gcttagatgc tttcagcact
      361 tatctcttcc gcatttagct accgggcagt gccattggca tgacaacccg aacaccagtg
      421 atgcgtccac tccggtcctc tcgtactagg agcagccccc ctcagttctc cagcgcccac
      481 ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta ccactttaaa
      541 tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga gccgacatcg
      601 aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt tatccccgga
      661 gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc actatgacct
      721 gctttcgcac ctgctcgcgc cgtcacgctc gcagtcaagc tggcttatg
//
LOCUS       DAZFKY010000500          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFKY010000500 DAZFKY010000000
VERSION     DAZFKY010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(182..724)
                     /gene="fimA"
                     /locus_tag="ACHKCL_005202"
     CDS             complement(182..724)
                     /gene="fimA"
                     /locus_tag="ACHKCL_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5765947.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          233 a          162 c          154 g          219 t
ORIGIN      
        1 gaaatataaa gaaatgattg ctttggtttc ttacaacaca tcattccctt ttcatttaat
       61 aaagcggcat gatacaaagt gaacatgatt tcgatttaca ttgccaccat accgcttcag
      121 acaaccggta cattactttg ttataagacg tgatatataa ttcaaaacaa cgtggttttg
      181 attattcgta tctcataata aaagtcgcgt cagcatttgc ttgccctgct gatgcacttg
      241 taccggtgcc tttataacgt gcagaaaaat gaagaacgtt ggtcccgggg atcaagttct
      301 ggttggttga gaagctattc ccatcaggtt tgagaattgc ggatgtatta tcaagtattt
      361 caataccgac acccgttgct gttgttgtat tggtactgga tgcaatggcc aataaattat
      421 cattgattgc atcagcctga ccagaaaatg caacggcagc attagcggca acaacaggat
      481 cgcagtcgtt caactgaatg gtgaatggaa ttaatgcaga ggtattacca acagcattga
      541 aaatatcggt acgatattga ccgagtgtga caacctggtc tgccgagtca gtattcactg
      601 aacaggcagc attcaccagt tcaccttcaa agtgaattgt accgccatct acaacggcag
      661 cataactacc cgtagcggcg aatagtgcgg cagccagcgc agacgaaata aatcttaatt
      721 tcatatatat tccttcaatc tcatttatcg actccacatc cgtatata
//
LOCUS       DAZFKY010000501          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFKY010000501 DAZFKY010000000
VERSION     DAZFKY010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            27..>767
                     /locus_tag="ACHKCL_005203"
     CDS             27..>767
                     /locus_tag="ACHKCL_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765948.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDAN"
BASE COUNT          287 a          154 c          131 g          195 t
ORIGIN      
        1 aaaacagata atgaataagg aatgttatgt caattgactt taccccaggt atgataaata
       61 catatcacgg cgatatttat aaccgcacaa cagataccga caatgttaaa acaccagaca
      121 caccgatgtg gccttgtgat aatcgggaag aacaacaacc cattaattcc acattctctg
      181 gagaaggata caaccctgaa caatatgatt tagcgcggca acacctccca caaatttatg
      241 cgtgccgcac gaacaccaaa tatacagatg cagactactc aaaagttgtg gctcaacttg
      301 taagtcttat tactaacatt gaatcgataa gttcgacacc acttacgcgg caggcacagg
      361 aaatacttaa tcaaattaat aacattagat acgaaaagaa caaaagtgct gaatgtcgaa
      421 tcattgttgt cgctaatcct aaacccgaca aggcaatcat aactaaaatt agcgtagaag
      481 agggaatacc agtaagattc agtgtccaaa ccatgttttc agatactaat tttattgctg
      541 aacaacgagc tgacttaccg actaatataa aagatattca aagcctgtat caaaaaatga
      601 cgaagttata tattgagcac agtgaaaata aaaacaggat gaaagtcttt gccggaacca
      661 actttattga tttcaatatg acaggacaga acctgtccgg gttcgttcta accttgtcaa
      721 ggttttattt cgaagatcta cttaatataa atttcactga cgcaaat
//
LOCUS       DAZFKY010000502          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFKY010000502 DAZFKY010000000
VERSION     DAZFKY010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>767
                     /locus_tag="ACHKCL_005204"
     CDS             <1..>767
                     /locus_tag="ACHKCL_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5765949.1"
                     /translation="AGYQYVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQ
                     SRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGL
                     GNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAAD
                     DLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQ
                     LALVGQNTELAKLKYQTALGELKTLTEMQKQELLRNA"
BASE COUNT          209 a          175 c          247 g          136 t
ORIGIN      
        1 tcgcaggata tcagtatgtt ctggcgaacc cgggctggac gaccggtgac ggattcatga
       61 taaaccatct gacatcggtg aagaccgtaa cggaagggct tgctcaggca acagagcagc
      121 ttgccgttga gcagtcccgt ctggcacaga tgcaggaaaa agcgcagtcc attcaggatg
      181 tgcttgccgg gctggaagac cgtcgtgtgg cgttaattcg tcagcaggcg gcagagcaga
      241 ataaggtgta ccagtccatg ctggttatga acggtcagca tacggaattc aaccgtctgc
      301 tggggctggg gaatgaactg cttcagcagc gtcagggact ggtgaatgta ccgttacggc
      361 tgccgcaggc caccctggat gataaacagc agagtgccct gacaaaaaca gagcgtgagc
      421 tggccctgtc cagactgaaa ggggaagaaa aagagcgtgt ccgactgggg tatgcggcgg
      481 atgacctcgg ttttgtgggt gatccgtatc aggaggcgag acaacgttat atcagtaatg
      541 ccctggaagc ctggcgcaat aacgaggcga ataaacccaa atcccggggt ggaaaatcag
      601 agacggaaaa agcggaagac agtttttccc ggctgctgaa gcagcagaaa gagcaactgg
      661 cactggtggg gcagaataca gagctggcga agctgaaata ccagacagcg ctgggtgaac
      721 tgaaaaccct gacggagatg cagaagcagg aactgctgcg taacgcg
//
LOCUS       DAZFKY010000503          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFKY010000503 DAZFKY010000000
VERSION     DAZFKY010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>766)
                     /gene="espY3"
                     /locus_tag="ACHKCL_005205"
     CDS             complement(<1..>766)
                     /gene="espY3"
                     /locus_tag="ACHKCL_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO5765950.1"
                     /translation="FDRLSPKQKQTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGEN
                     LMIRGTISIVNEVVKTYSLPIEKDDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDM
                     GNCNCSNVNFEHTYFYGVKFTNANCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGK
                     EKEPEMQYPEADQIIQRITYQKSDGNETKGMILTNCSCVKTTFNWADLSESDCQNVDF
                     SEANLSNTILPDIVRMKGTKLYRTDLFNPILKTEAES"
BASE COUNT          209 a          137 c          141 g          279 t
ORIGIN      
        1 ttgattctgc ttctgttttt aatatcggat taaaaaggtc ggtacggtaa agtttcgtgc
       61 ctttcatcct gacgatgtca ggaagaatag tgtttgaaag attagcctcg ctaaagtcca
      121 cattttggca atcactttct gataaatctg cccaattgaa agtggttttc acacaggagc
      181 agttagttag aatcatccct ttcgtttcat tcccatctga tttctgataa gttatccttt
      241 gtattatttg atctgcttct ggatattgca tctcaggttc tttttcttta ccatatatca
      301 tcgcattatc aagaattgcg ccagtgcaat tcatatttgt caggtcgcat ttcttaaatc
      361 tacaattgtt aaaattcgca tttgtacaat tcgcatttgt aaacttgacg ccataaaaat
      421 aagtatgttc gaagttgaca tttgagcagt tacagtttcc catatctata ccgaaatcta
      481 tatcgggatc tttattggga tctttaccgg aaatattgct gtgtgctaga tatagaccgc
      541 tgaaatcgta atatccattg tcatcctttt ctattgggag tgaataagtc tttactacct
      601 cgttaacaat agagatagtt ccacgtatca tcagattttc tccgtcttta taaaaaataa
      661 tgttatgttt gatggtactg ttgtatggct ctggcaatga ggatgcaatt ttattccaga
      721 aacaacaaag cgtggtctgt ttttgtttgg gcgagaggcg atcaaa
//
LOCUS       DAZFKY010000504          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFKY010000504 DAZFKY010000000
VERSION     DAZFKY010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..488
                     /locus_tag="ACHKCL_005206"
     CDS             <1..488
                     /locus_tag="ACHKCL_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5765951.1"
                     /translation="TETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAY
                     AFDYHEKGNVTDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYNYS
                     PYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDASVKSKAQAYIELLTDNSEDN
                     G"
BASE COUNT          287 a          119 c          121 g          227 t
ORIGIN      
        1 acacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
       61 cattatcgaa aggagaaaat ctggcattac tacatggttt gactcctgat atccttgata
      121 gaatatatgc atatgcattc gactaccatg aaaagggtaa tgtaacggac gcagaaattt
      181 attataaatt attgtgcatt tatgcgttcg aaaatcatga atatctaaaa ggttttgcat
      241 cagtatgtca gtcaaaaaag aaatatcaac aagcatatga cctttacaag ctaagttaca
      301 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt caaattgggg
      361 ctaaaaatat cgataacgca atgcaatgtt tctatcatat tattaacaat tgtgaggatg
      421 ctagtgttaa gagtaaagcg caggcatata ttgaactctt aaccgataat tcagaagata
      481 atggctaagc attacaatca tttggttaaa aatgctggtt atagtgtagt aaccactatg
      541 accagcacct cttatgcttt cttaaatcaa catcttaaaa cagttaagaa cacactaaaa
      601 aatattattt ttcaacgcgt tagagaacaa tctattttat caaaattgaa taaggctatt
      661 tcagattaca tcagtgaatt cctaagataa attagtgaac acaaccatag atatgtcgaa
      721 tgtaaactcc tgtagcgtta atgacacata aata
//
LOCUS       DAZFKY010000505          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFKY010000505 DAZFKY010000000
VERSION     DAZFKY010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>754
                     /locus_tag="ACHKCL_005207"
     CDS             <1..>754
                     /locus_tag="ACHKCL_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765952.1"
                     /translation="DHITIGIDNQEPIKLPASYKDKELVRTIINDNIVEKTHDDINNA
                     EFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIKSPANDENKKVGQTIINENIVENTHDI
                     NNKKMIFSAIKEIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTGLFTWYGKDKKGD
                     SLAIVIKNKNGNDYLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARSASAWFEHSKA
                     IIAESFATGSDHQVVNELKGERLRELNEAFNPS"
BASE COUNT          294 a          131 c          139 g          190 t
ORIGIN      
        1 gaccatatta ctattggtat cgacaaccaa gaaccaataa aattacctgc gagttataaa
       61 gacaaagaac tcgtccgtac tatcattaat gacaacatag ttgagaagac tcatgatgat
      121 ataaataatg ctgaatttac tgatggtggt attgatgatc gtagtgatat tggcattgac
      181 tccaatgata cagacgatat tgatattgat aatcaagaac caataaaatc acccgcgaac
      241 gatgaaaaca aaaaagtcgg ccaaaccatc atcaatgaaa acatagtaga gaatactcat
      301 gatatcaata acaagaaaat gatcttcagc gcaataaaag aaatatatga tgaagatcca
      361 agttttatct tcgataaaat atcacacaaa ctcagacata cggtaacgga attcgatgag
      421 agcgggaaaa gcgaaccaac gggcttattt acctggtacg gtaaagataa aaaaggcgac
      481 tctctcgcta ttgtaattaa aaataaaaat ggaaatgatt acttatctct cggttactac
      541 gatcaggatg gctaccacat tcaaaaagga tttcgtattg atggtgataa tctcacccaa
      601 tattgtagta aaaaagccag gagtgcttct gcgtggtttg aacacagtaa agcaatcatt
      661 gcagaatcat ttgcaacagg ttccgatcat caagttgtaa acgaactcaa aggggaaaga
      721 ctgagagaac ttaacgaagc ttttaatcct tcaa
//
LOCUS       DAZFKY010000506          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFKY010000506 DAZFKY010000000
VERSION     DAZFKY010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..112
                     /locus_tag="ACHKCL_005208"
     CDS             <1..112
                     /locus_tag="ACHKCL_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765953.1"
                     /translation="NPTASQNRSPAPVFVVMSGGCLLGKQRWQNCWRFCG"
     gene            180..>741
                     /gene="nleB2"
                     /locus_tag="ACHKCL_005209"
     CDS             180..>741
                     /gene="nleB2"
                     /locus_tag="ACHKCL_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO5765954.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRN"
BASE COUNT          253 a          103 c          164 g          221 t
ORIGIN      
        1 taacccgaca gcttcacaaa accggagtcc ggctccggtt tttgttgtca tgtccggtgg
       61 atgtttgtta ggaaagcaaa gatggcaaaa ctgctggagg ttttgtggtt gagtatgcca
      121 atataattaa tagattaaag agttagttgt gaagaaaata tggataaaca ggacgacgaa
      181 tgctttcacc gataaggaca actttccata actcagtaaa tatagtgcag agttcaccct
      241 gtcaaacggt ttcttttgca ggaaaggaat atgagttaaa ggtcattgat gaaaaaacgc
      301 ctattctttt tcagtggttt gaacctaatc ctgaacgata taagaaagat gaggttccaa
      361 tagttaatac taagcagcat ccctatttag ataatgtcac aaatgcggca aggatagaga
      421 gtgatcgtat gataggtatt tttgttgatg gcgatttttc agtcaaccaa aagactgctt
      481 tttcaaaatt ggaacgagat tttgaaaatg taatgataat ctatcgggaa gatgttgact
      541 tcagtatgta tgacagaaaa ctatcagata tttatcatga tattatatgt gaacaaaggt
      601 tacgaactga agacaaaaga gatgaatact tgttgaatct gttagagaaa gagctgaggg
      661 aaatttcaaa ggcgcaggat tctttgattt ctatgtatgc aaagaaaaga aatcatgcat
      721 ggtttgattt cttcagaaat t
//
LOCUS       DAZFKY010000507          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFKY010000507 DAZFKY010000000
VERSION     DAZFKY010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..723
                     /locus_tag="ACHKCL_005210"
     CDS             <1..723
                     /locus_tag="ACHKCL_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO5765955.1"
                     /translation="DRNAGSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGF
                     NELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQ
                     RLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEI
                     DNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGV
                     IADWPVNRVSELLPWRIALPAE"
BASE COUNT          175 a          196 c          224 g          144 t
ORIGIN      
        1 gaccgcaatg cagggtcagc gttggcacct gcagtgtggt tcgcttacag cccggacaga
       61 aaaggcatcc atccgcagac tcatcttgcc tgcttcagcg gtgtgctgca agcggatgcg
      121 tacgccgggt tcaacgagct gtatcgcaat ggtgggataa cggaagctgc ctgctgggct
      181 catgcccgcc gaaagatcca cgatgtgcac gtccgcatcc cgtcagcact gacggaagaa
      241 gccctggagc agatcggtca gttgtacgcc atagaggcgg atataagggg aatgccggca
      301 gagcagcggc ttgctgaacg tcagcgaaaa acgaaaccgt tgttgaaatc cctggaaagc
      361 tggttgcgtg aaaagatgaa gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg
      421 tacgcactta accagtggcc ggcactgacg tactatgcga acgatggctg ggtggaaatc
      481 gacaacaaca tcgctgaaaa tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg
      541 ttcttcggct ctgatcatgg tggtgagcgg ggagcgctac tgtacagcct gatcgggacg
      601 tgcaaactga atgacgtgga tccagaaagc taccttcgcc atgtgcttgg cgtcatagca
      661 gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact gccagctgaa
      721 taacacatcc ccgtcaata
//
LOCUS       DAZFKY010000508          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFKY010000508 DAZFKY010000000
VERSION     DAZFKY010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..287
                     /locus_tag="ACHKCL_005211"
     CDS             3..287
                     /locus_tag="ACHKCL_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765956.1"
                     /translation="MECHHTEYNFILTAELYVDEFTAGRERKRFEFTGVEHLSQVMDI
                     AELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFKTMRVIDNECA"
     gene            274..705
                     /locus_tag="ACHKCL_005212"
     CDS             274..705
                     /locus_tag="ACHKCL_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765957.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQLQFIILGDDKIRIWVPEGKLYFALMNVLQMFPMKNG"
BASE COUNT          256 a          101 c          158 g          223 t
ORIGIN      
        1 gaatggaatg tcaccacacc gagtataatt ttatattgac ggcggagctt tatgttgatg
       61 aatttacagc cggaagggaa cgaaaaaggt tcgagtttac aggagtggaa catttatcgc
      121 aggttatgga tattgctgaa cttatagata accaaaatgc aggtaatatt aaagatgcga
      181 caattgttta taaggcaaag aagtataaaa tttcacttca tttgtttggt ggttttttaa
      241 gttttacatt taaaaccatg cgagttattg acaatgaatg tgcgtaatgt caaattcgac
      301 aacttgtctt tttatgaaga aaaaatccgc tggcgaatgc gtcggcaaat ctcgaatgca
      361 gaggcaatat tttacgagaa tcaggaagaa cacattcggc gctttttagg cagatatacc
      421 tgtcgcaaac cggttcttgt ttattgggaa agtgaacaaa gatggacatc tgtaaacggt
      481 ctggaagtga tgagtatgtt tggtgaacat tgtcatcaca tctaccttga tgatatcaag
      541 aaaaaaatac aaactgaatt acctgaaaat aaaactttag atttcaaacg tcaattgcaa
      601 tttataatat taggggatga taagattaga atatgggtgc ctgaggggaa attgtatttc
      661 gcattaatga atgtattgca gatgtttccc atgaaaaatg gttaataact ggacaagata
      721 attttaaaat tcagcaac
//
LOCUS       DAZFKY010000509          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFKY010000509 DAZFKY010000000
VERSION     DAZFKY010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>730
                     /locus_tag="ACHKCL_005213"
     CDS             <1..>730
                     /locus_tag="ACHKCL_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5765958.1"
                     /translation="TEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTE
                     RELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEVNKPKSR
                     GGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLR
                     NAALIDQQKIREQLRYREETLKNDNVAARASNESELLGYGQGERARERMRELQQIRDS
                     FRQKDADLQSQYQTGDISEDFYRQA"
BASE COUNT          204 a          166 c          239 g          121 t
ORIGIN      
        1 atacggaatt caaccgtctg ctggggctgg ggaatgaact gcttcagcag cggcagggac
       61 tggtgaatgt gccgttacgg ctgccacagg ccactctgga tgataaacag cagagtgccc
      121 tgacaaaaac agagcgtgag ctggccctgt ccagactgaa aggggaagaa aaagagcgcg
      181 tccgactggg gtatgcggcg gatgacctcg gttttgtggg ggatccgtat caggaggcga
      241 gacaacgtta tatcagtaat gccctggaag cctggcgcaa taacgaggtg aataaaccca
      301 aatcccgggg tggaaaatca gagacggaaa aagcggaaga cagtttttcc cggctgctga
      361 agcagcagaa agagcaactg gcactggccg gtcagaacac ggagctggcg aagctgaagt
      421 accagacagc gctgggtgaa ctgaaaaccc tgtcggagat acagaagcag gaactgctgc
      481 gcaatgcggc cctgattgac cagcaaaaaa tccgggagca gttgcggtac cgggaagaga
      541 ccctgaagaa tgataatgtg gctgcgcgtg catcaaatga atctgaactg ctggggtacg
      601 ggcaggggga acgagccagg gaacgcatgc gggagttgca gcagatccgc gacagcttcc
      661 gccagaagga tgcggacctt cagtctcagt atcagaccgg ggatatcagt gaggattttt
      721 acagacaggc
//
LOCUS       DAZFKY010000510          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFKY010000510 DAZFKY010000000
VERSION     DAZFKY010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>729)
                     /locus_tag="ACHKCL_005214"
     CDS             complement(<1..>729)
                     /locus_tag="ACHKCL_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5765959.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIR"
BASE COUNT          110 a          260 c          183 g          176 t
ORIGIN      
        1 ccttattatc ggattcagtg ggtaattata cagattccat ccccccgcca gcccaatggg
       61 gtcttgtgta atatacctcc cctgtagcgg gtcatagtac cggttccggt tatagtacag
      121 ccctgattcc tcatcatgct gctgccccgg cagacggtat ggctggtgca ggtaatacgg
      181 gttctcttca ttaagctggt tgccccattc atcatactcc ccgcgccacg ccgtattgcc
      241 gtcttcgctg atgagcgcca gcggcaggcc ccggtggtcg cagtggtaaa aatgaacttt
      301 tcgcgccggt gtgtattccg gctccacctg tctggccagt tgctccaccg tcagcccgca
      361 ctgcgcaagc cacgcccggc tttcactgct cacgcggtct gcccggattt cttcctccag
      421 cctgtccagc agccgcacca gttcagccgg gaacaccacg ccgtggccgt tctcactccc
      481 ttcctgctgg agcgtctctg ccaggctgcg ccgctgcgct ttttcccgct cgccgttctc
      541 tgtctcgacc cggatgagcg gcgtgaagct tcccggctcg tataccgtct ggatacgtgt
      601 ggtgtcagtc tgcaccgtcg tcagcctgtc tccgtcccag ccataccacg tcacctccgg
      661 tttacgcgac agcgacatcc accccgtcag gtcacgctcc cgccgccaga cccgttttgc
      721 cattcgccg
//
LOCUS       DAZFKY010000511          718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFKY010000511 DAZFKY010000000
VERSION     DAZFKY010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..>718
                     /locus_tag="ACHKCL_005215"
     CDS             52..>718
                     /locus_tag="ACHKCL_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5765960.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNTKTTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDSLAQMQYWTAVRNIIDDTNEVTIDLSYNLAIT
                     NIDTSDEHLVEVSDNSEGNYIKDNDSMSIRYRSKYYSRDSALIEEETIFSDAELKAIL
                     PMRRMYGVSDYKSNSSSLSSHSGLKDPTGTPVCYYIHNESKPSLGYGPTSNNWLSQSF
                     TTEL"
BASE COUNT          257 a          148 c          108 g          205 t
ORIGIN      
        1 ctggatgtct cccggcaaga gtctctgatt gagttaagtg cccatcacaa tatgatcact
       61 gaccttatat tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt
      121 gcacatttaa acaccaaaac tactacaaaa cttgaatatt taaacttaag caataacaat
      181 ttattgccaa ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta
      241 gttaacggca tcaacaatga ttcacttgca caaatgcagt actggactgc agtaagaaat
      301 ataattgatg acactaatga agtgaccatt gatttatctt ataatctggc aatcacaaat
      361 atcgatacca gtgatgaaca tcttgtagaa gtaagcgata attcagaagg taattatata
      421 aaagacaatg actcaatgtc tattcgttat agatcaaaat attattccag agattccgcc
      481 ttaatagaag aagaaacaat attttctgac gcagaactaa aagctattct gcctatgcgt
      541 cgcatgtacg gggttagtga ctataagtca aattcctctt ctctatcctc acactcggga
      601 ctcaaggacc caacgggcac acccgtctgt tattatattc ataatgagag taaaccttcc
      661 ttaggttatg gtccaacatc caataattgg ttaagccaat cctttacaac agagttat
//
LOCUS       DAZFKY010000512          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFKY010000512 DAZFKY010000000
VERSION     DAZFKY010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..232
                     /locus_tag="ACHKCL_005216"
     CDS             <1..232
                     /locus_tag="ACHKCL_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO5765961.1"
                     /translation="DLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEARG
                     AWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            189..635
                     /locus_tag="ACHKCL_005217"
     CDS             189..635
                     /locus_tag="ACHKCL_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5765962.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPRRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            632..>714
                     /locus_tag="ACHKCL_005218"
     CDS             632..>714
                     /locus_tag="ACHKCL_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5765963.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDI"
BASE COUNT          193 a          170 c          205 g          146 t
ORIGIN      
        1 cgacctatca ttaacccagc caaagctaaa agaatgtccg ttttgcggcg gtaatgctcg
       61 tctgtgggtt gaggccggaa taaatattga tgtgtggggc tatgcagaat gtgacctctg
      121 tgaagccagg ggggcatggg caccatcagt tgctgcggcg gctgaaaaat ggaaccggag
      181 agcaggagat gaagcaaacc tttctgcttc gcaacgaagc aatcagaaat aacgccatag
      241 acgccattct ctcactaccc atcgacgaca agtcacccca cgaagtccac gttaaagaac
      301 ccaggcgcag caaagcgcag aatgaccgta tgtggccgat gctgaacgat gtttcgcgtc
      361 aggtgctatg gcatggtcaa cggctggcgc cggaagactg gaaagacctg ttcactgccc
      421 tgtggcttaa gaccaaaaaa ctggagcaac gaagtgtgcc tggtatcgac ggtggcgttg
      481 tcatgcttgg cgtgcgtacc agcaaaatgc ggaaggccag catgactgag cttatcgaaa
      541 tcatgttctg gttcggctca gagcgcaacg tgcggtggag tgatgactcc cggcgagagt
      601 atgaatggtc acaacgaaaa ggtagggctg catgactatc aaatcaaata cgccagcaca
      661 cgacaaggac tgctggcaaa cgccgctttg gctttttgat gcactggata ttga
//
LOCUS       DAZFKY010000513          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFKY010000513 DAZFKY010000000
VERSION     DAZFKY010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>710
                     /locus_tag="ACHKCL_005219"
     CDS             <1..>710
                     /locus_tag="ACHKCL_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5765964.1"
                     /translation="KGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIR"
BASE COUNT          165 a          185 c          227 g          133 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgccggtt gaggcaaagg acaatcttaa gtccacgcag
       61 atgatgagcg tgattgatgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactgttg
      361 gagaccacct caaagggcga ccgtaatccc tcttctgtcc gactgctgat tcagttgcag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccgtc cttttaacat ccggatggtc
      541 cgggagacgg cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agtttggtgg ccagcagatg acggtgaact accatatccg
//
LOCUS       DAZFKY010000514          703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFKY010000514 DAZFKY010000000
VERSION     DAZFKY010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>703
                     /locus_tag="ACHKCL_005220"
     CDS             <1..>703
                     /locus_tag="ACHKCL_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5765965.1"
                     /translation="TPYTLMQAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGL
                     PLPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLS
                     KGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGIFSESTGASQDSA
                     RWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQ
                     FRADLSVFNAQCHGG"
BASE COUNT          153 a          183 c          218 g          149 t
ORIGIN      
        1 taacccctta taccctgatg caggcgatta cagcagaagg tgatgtggtg gtcagtggtg
       61 caactgagcc ggattggtac tacgtcatcg tactggccgg gcagtccaat gccatggctt
      121 acggtgaagg gcttccgctg ccggattcat acgatgctcc ggatccgcgc attaaacagc
      181 tggcgcgccg cagtacagtg acgccgggtg gggctgcctg cagatataac gatattattc
      241 cggccgacca ctgcctgcat gatgtgcagg atatgagtac gctgaatcat ccgaaggcag
      301 acctgagcaa agggcagtac ggctgtgtcg gccaggggtt acatattgcc aaaaaactgc
      361 tcccgtatat cccgaataac gcggggatcc tgctggtacc atgctgtcgt ggtggttcgg
      421 catttaccca gggcgcggag gggatattca gcgagtccac gggggccagc caggattcgg
      481 cgcgctgggg tgtgggtaaa ccgttatatc aggacctgat tgcgcgcacc aaagctgcat
      541 tacagaagaa cccgaaaaat gtgttgctgg cggtgtgctg gatgcaggga gagtttgaca
      601 tgagcgccgc cacccacgca cagcaacctg cgctgtttac agccatgctg acacagtttc
      661 gtgctgacct ctccgtgttt aacgcgcagt gccatggtgg cag
//
LOCUS       DAZFKY010000515          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFKY010000515 DAZFKY010000000
VERSION     DAZFKY010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..632
                     /locus_tag="ACHKCL_005221"
     CDS             <1..632
                     /locus_tag="ACHKCL_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5765966.1"
                     /translation="GKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIAE
                     CNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAKG
                     CVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIAD
                     GKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          239 a          107 c          149 g          206 t
ORIGIN      
        1 tgggaaaaat taaaattgta gtttcagatc agcagccgtt tatgattgat gggataattg
       61 gattcctcgg acattatccc gatttatatg aggttgttgg gggctataaa gatctgaaga
      121 aagctatagc ggagtgtaac aaatctgcag cccaaatatt tattttagga gaatttgcag
      181 gtggaatgat gggggccgaa ctcgtaaaat gggttaaatc gcataaaata gatgctcata
      241 ttattacatt tgtagcaaaa atgccgtata ttgattcaat aaaattgctt gaagcaggtg
      301 cgaaaggatg cgtatggaaa accagtcacc cggcgaaact taatcgcgct attgattcga
      361 ttagtaacgg ctacacttat tttgatagtg tacatatgga ttgtgagaaa atatcttcca
      421 gatattcctc tgataatcaa cttacaaatc gtgagtctga aatattacaa ctcatagctg
      481 atggaaaaac aaacaaagaa atcgctaatt tcttgcagtt aagcaggaaa acagtcgaaa
      541 ctcatagact caatatcatg aagaaattag atgttcacag tggtatcgag ttgatcaaaa
      601 cagctttacg tatgggtgtg tgcactatat aaaaaaaaga ggctttaggc tcattttgct
      661 aaagccttac gattgtctag ttcattaagc ctggtatata c
//
LOCUS       DAZFKY010000516          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFKY010000516 DAZFKY010000000
VERSION     DAZFKY010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..541)
                     /gene="fimE"
                     /locus_tag="ACHKCL_005222"
     CDS             complement(<1..541)
                     /gene="fimE"
                     /locus_tag="ACHKCL_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5765967.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFA"
BASE COUNT          214 a          170 c          140 g          162 t
ORIGIN      
        1 cggcaaaacg agcagcatta ctggcggtat aacgtacagt atggcgaata tttcgatgcc
       61 cgagataatc ctgaattaaa cgagtatctg caccacgctc cgccagttca taaccgcaag
      121 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcac
      181 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatgg
      241 cgtcggtccg gtcagcgcct ttccagttag cacgttctag ggtccagcgt tccacagctt
      301 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
      361 ttattcggcc ttcattaagg tcaagatcct gataatgcag atcaagcagt tcactaatac
      421 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
      481 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
      541 caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg
      601 gtcaaaatta gcccaataga taaaaagata accacacacg aagtgatgtg gctatcagtc
      661 aattacatta aatatcatac aaaaat
//
LOCUS       DAZFKY010000517          685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFKY010000517 DAZFKY010000000
VERSION     DAZFKY010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>685
                     /gene="rfbE"
                     /locus_tag="ACHKCL_005223"
                     /gene_synonym="perA"
     CDS             <1..>685
                     /gene="rfbE"
                     /locus_tag="ACHKCL_005223"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO5765968.1"
                     /translation="AIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFGSKYKGKY
                     VGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQYWHDVIGY
                     NYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVFHTYWMVS
                     ILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGWRGINLPS
                     FPSLSNEQVI"
BASE COUNT          230 a          110 c          140 g          205 t
ORIGIN      
        1 gctattatgt gtgtccattt atacggacat ccatgtgata tggaacaaat tgtagaactg
       61 gccaaaagta gaaatttgtt tgtaattgaa gattgcgctg aagcctttgg ttctaaatat
      121 aaaggtaaat atgtgggaac atttggagat atttctactt ttagcttttt tggaaataaa
      181 actattacta caggtgaagg tggaatggtt gtcacgaatg acaaaacact ttatgaccgt
      241 tgtttacatt ttaaaggcca aggattagct gtacataggc aatattggca tgacgttata
      301 ggctacaatt ataggatgac aaatatctgc gctgctatag gattagccca gttagaacaa
      361 gctgatgatt ttatatcacg aaaacgtgaa attgctgata tttataaaaa aaatatcaac
      421 agtcttgtac aagtccacaa ggaaagtaaa gatgtttttc acacttattg gatggtctca
      481 attctaacta ggaccgcaga ggaaagagag gaattaagga atcaccttgc agataaactc
      541 atcgaaacaa ggccagtttt ttaccctgtc cacacgatgc caatgtactc ggaaaaatat
      601 caaaagcacc ctatagctga ggatcttggt tggcgtggaa ttaatttacc tagtttcccc
      661 agcctatcga atgagcaagt tattt
//
LOCUS       DAZFKY010000518          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFKY010000518 DAZFKY010000000
VERSION     DAZFKY010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            136..354
                     /gene="tdcR"
                     /locus_tag="ACHKCL_005224"
     CDS             136..354
                     /gene="tdcR"
                     /locus_tag="ACHKCL_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO5765969.1"
                     /translation="MSKFSNFIINKPFSAINNAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            610..>684
                     /locus_tag="ACHKCL_005225"
     CDS             610..>684
                     /locus_tag="ACHKCL_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765970.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHN"
BASE COUNT          225 a          125 c          124 g          210 t
ORIGIN      
        1 tcaaaaaaaa tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt
       61 aatacaaaaa aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa
      121 tttgagttaa atttaatgag caagttctca aattttatta taaataaacc attttcagcg
      181 ataaataatg cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt
      241 tatcaatatt ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc
      301 aactttttct cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt
      361 cttccttcat aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg
      421 atcgccgcga taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgg
      481 cgtaggccac agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat
      541 agtgcgaaag aaccctgcga ggaaaataag catctattat tgtttgtcat gatgacaagg
      601 caggcatcaa tgaaaggctt cccaattgca catatttttc acccttcaat cccaccaatg
      661 cacgcagtgg ttaacaatca caat
//
LOCUS       DAZFKY010000519          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFKY010000519 DAZFKY010000000
VERSION     DAZFKY010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>681
                     /locus_tag="ACHKCL_005226"
     CDS             <1..>681
                     /locus_tag="ACHKCL_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5765971.1"
                     /translation="SLIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIMTAS
                     CPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGKESA
                     WGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLAPFG
                     ISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRLHRCDRPSIKRD
                     LVDYNIINE"
BASE COUNT          226 a          104 c          160 g          191 t
ORIGIN      
        1 tcattaataa aagccatact tatagcacaa agggcaaatg aagaaagcga gataaagtca
       61 agtcgggtta aattatcatg gaagaaaaaa cggcaggatg cactggaatc aggtacgatt
      121 atgacggcgt cttgtccgag atggctctcc ttagatgaca aaagaacggc ttttgttcca
      181 gaccccgaca gggtgaaaac tattgagcta atttttaaac tcaggatgga aaggcgctca
      241 ttgaatgcaa tagccaagta tttaaatgat catgctgtaa agaatttctc aggaaaagaa
      301 agtgcatggg ggccttctgt aattgaaaaa ttattagcga ataaagctct gataggtata
      361 tgcgtacctt catatcgtgc aagagggaaa gggataagtg aaatcgctgg ctattatccc
      421 agagtcatat cagatgattt gttttacgct gtacaggaaa ttcggttggc accttttggt
      481 attagcaata gtagcaagaa tcctatgcta ataaatctac ttcgaacagt tatgaagtgt
      541 gaggcttgtg gtaataccat gattgttcat gcggtatctg gaagtttgca tggctattat
      601 gtttgtccga tgagaagatt acatcgatgt gacaggccat caataaagag agatttggtt
      661 gattataata tcattaatga a
//
LOCUS       DAZFKY010000520          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFKY010000520 DAZFKY010000000
VERSION     DAZFKY010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..628)
                     /gene="lpfA2"
                     /locus_tag="ACHKCL_005227"
     CDS             complement(26..628)
                     /gene="lpfA2"
                     /locus_tag="ACHKCL_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO5765972.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          192 a          151 c          136 g          199 t
ORIGIN      
        1 tttaaggtat tatgttaaga atgatttatt ttatatattc aactgtaaac tgcgagtcgg
       61 cgttagctgt accagggtca atagtcgttt ccgtagcaat ataacgagcc atgaacttca
      121 tggatgcatt accattagaa atcggagttg atgcagaatt ttcatataac ttgacctgag
      181 tgttatctgc acggttatat aaacgaatag ctacgccttt cgctgcacta acggcacccg
      241 caccagtata atccccactg ttatcactcg ggctaatacc agcggcagca tcgttagagt
      301 tatccagcgg ggtaccaatt gccaggttgc cattaccatg agcatcttca ttaccatcga
      361 aacgaattgc ggcggactta aaggtttccg ggcattgagt cagatcgata gaaaaagccg
      421 tcggggcagc cacatcatcg acgccactaa aggctgtacg gtttactttg cctaaatcaa
      481 cgacctgatc tttagtttca ggagtgactt cgcaagtact gtcaataatt tcaccacgga
      541 aatggactgt tccatcagcc gcctgtaggt gacctgccat aaacacagaa gttaacgcta
      601 atgctcctac aatcatattt ggtttcataa atataactcc attaatttga tattattcct
      661 gtgtgttatc catttatt
//
LOCUS       DAZFKY010000521          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFKY010000521 DAZFKY010000000
VERSION     DAZFKY010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(70..675)
                     /gene="map"
                     /locus_tag="ACHKCL_005228"
     CDS             complement(70..675)
                     /gene="map"
                     /locus_tag="ACHKCL_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFO5765973.1"
                     /translation="MTMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQSN
                     FVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVISK
                     ETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFNTQ
                     TKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          163 a          157 c          132 g          224 t
ORIGIN      
        1 ttttttctta catcaacaaa ggccatgcta tacaccagga agcttaattt atacctgaca
       61 aggttatccc tacaatcggg tatcctgtac atgctctaaa atcgcattct gagtaatcgc
      121 ttttatctca ctgactttga agccgctaaa gttgcgttca aatatttctt gtgagacttg
      181 ttctatcatc ttcgtctggg tgttaaaacg tgttattgga tccactcttt tgccgcactg
      241 ctcatcaact ttctgattaa agtatttatt ggactgcaag aagtgaccaa ttgatgattg
      301 tgccccgttc atatcaagag gttgctgtaa cgcattttca acagctctaa aaatgtgacc
      361 tttagtctcc ttactaatga ccgaattatt actcctgcaa tagtcatcca gagttctatt
      421 tactgttctg gatatataag tcgtctgctc ctgctggaac cattgttggg tatcgccatt
      481 acgagtttgt ttggctacag cctgcaggag ttggttagtc agtttgccat gattaatcac
      541 aaagttcgac tgcacgggca tgaacctcat acccgtggct cccgtacccg cttgcattgc
      601 agctctggta accgcaggtt taagcgtctg tgcagtcgcc tgaactaacg atctgcctgc
      661 cattgtcatt gtcatt
//
LOCUS       DAZFKY010000522          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFKY010000522 DAZFKY010000000
VERSION     DAZFKY010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..589)
                     /locus_tag="ACHKCL_005229"
     CDS             complement(<1..589)
                     /locus_tag="ACHKCL_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFO5765974.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          191 a          162 c          148 g          172 t
ORIGIN      
        1 taactgagtg ataatattca agataaccac cggttaaatt taacagccga cgcgccatcg
       61 catcgccaga aaatgttcca ccagaaacag ccgtcccgac atggacataa cgtgaagctg
      121 ggccatattg ttgcgtgatc cttttcaggt tatcggcaat taaggtcgtc gcctcatccc
      181 atgagatacg ctcaaattta ccctcgcctc gtttacctac ccgcttcatc ggatatttca
      241 aacggtctgg atgataaaca aatttacgat agccgcgacc acgaacacag gcgcgcataa
      301 tcggcatttg tggatccaat tcgttatcgg gaagcgtggt aatttgcgtc accacgccgt
      361 cgcgcatatg cgcccgaata tcgcacttac caccgcagtc aaatgtgcta caggtgggca
      421 cgactttatc agctactgga gctgtaactt ctggcgacgc atctggggat tgcgcctttg
      481 cggtagaaga aataaaaggc aaagtgataa gcgcggaagt cgcttgtaaa aaactacgtc
      541 gtgatacttg cggaaatgta atgcgttcct tttcgtctgg cgtatccatc ttgcgttccc
      601 gaaaatgaaa aaataaatat tcacgtattt attcgagaac aatgctgata acaatcaaat
      661 aattagtcgt tta
//
LOCUS       DAZFKY010000523          672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFKY010000523 DAZFKY010000000
VERSION     DAZFKY010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(372..>672)
                     /locus_tag="ACHKCL_005230"
     CDS             complement(372..>672)
                     /locus_tag="ACHKCL_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765975.1"
                     /translation="TSQDVSELLSKSLNGTISSTSGLKKSGNAFFGTGKYLKEIDFTI
                     NGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSVDEEKSKSDKIDFTFSGAATTADL"
BASE COUNT          216 a          141 c           89 g          226 t
ORIGIN      
        1 ttttgtcgat actctccctt agactctgat tttgaaactg gctattatca ttctctgttt
       61 ccgttggata tgaataactc gtcacactaa ccatagtaac ctcgcaatat gacactttgc
      121 atattttgac attgttagaa gtatcaatgg ctagcatgat aaatatctca atgctaaccg
      181 ctatatttca tttatttcaa taataagact atagtgccgt taatttttat cgatttcccc
      241 aatgcaattg catcactaat actacttatt cactatcaaa aatcgctcat tttttcaaaa
      301 agactgttta cacacagacg gttaacaata cgtgttcacc ttaatgataa acgcgcttta
      361 tcaagaaaaa atcataaatc agcagttgtt gcggcaccgg aaaatgtaaa atcgatttta
      421 tcgcttttgg atttttcttc gtcgacacta ccggtagtaa aataacaaga gcactgaagc
      481 ttcacttcat ttccaacatt attaaccgcc atcaaataca tcttctgact aaacccattt
      541 atagtaaaat caatttcttt aagatacttc ccagtcccaa aaaaggcatt tcctgacttt
      601 tttaaacccg atgttgatga aatggtacca ttcaaactct ttgagagtaa ctcgctaaca
      661 tcttgagatg ta
//
LOCUS       DAZFKY010000524          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFKY010000524 DAZFKY010000000
VERSION     DAZFKY010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>668
                     /locus_tag="ACHKCL_005231"
     CDS             <1..>668
                     /locus_tag="ACHKCL_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765976.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHT"
BASE COUNT          148 a          167 c          188 g          165 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacatac
//
LOCUS       DAZFKY010000525          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFKY010000525 DAZFKY010000000
VERSION     DAZFKY010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(20..>660)
                     /locus_tag="ACHKCL_005232"
     CDS             complement(20..>660)
                     /locus_tag="ACHKCL_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5765977.1"
                     /translation="FLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQGNIF
                     AAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFVAKERR
                     PSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKLLKFNL
                     TEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
BASE COUNT          184 a          125 c          135 g          216 t
ORIGIN      
        1 aataaggcca gtgacagact tacttattag ggacaaattt cgcgaggata tcaggttcac
       61 tggcaacata aatgctttca tcaggcagag ttacaattcc ttcaggttgg ggcaaatcac
      121 tggtcagaga atgatttcct tttgtcaggt caagcatctc aatgaaagac atctctgtca
      181 ggttaaactt gagtaattta cgcgattcat cggacagaat catcagactt tcattattaa
      241 aggccaggcc ggatatatcg ttaacacgta tatcatttag tgcctctgga atagttgctt
      301 gctttacact cagcaggtct ggagaaagtt gatatataaa tattttactt gggcgtcttt
      361 cttttgcgac aaacagcatc cgatcatctg ctttccatgc cagcccttca aaccctctat
      421 tctttttatt gaatttttct aactgaattt tatttcgaaa ggagatatgc atatcttttg
      481 taactgtaaa aaagaatagt tcagaagtct tttcgtctac tgcagcaaaa atgttccctt
      541 gtatgtgttc aattgtttcg gcatcagaga taaaaggcag tggggctctg tccatgatgt
      601 cgccactttt ggttatttta agtagctgtg cagggctatt ctgggtggca tacaggaaat
//
LOCUS       DAZFKY010000526          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFKY010000526 DAZFKY010000000
VERSION     DAZFKY010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          225 a          115 c          121 g          199 t
ORIGIN      
        1 gttcaaaata tcaggacttg gctaaaagaa aatcaaaata tggtgaaagc tattaagaaa
       61 attgatactg aataacatgg cgaataaata aacggatcaa gtcgttccta tgacaatgag
      121 gttatacgaa aagacgctta ctcatcagaa atctggtatg ctttaattaa accagtgtcg
      181 ctgatgtgta agcgttgacg ttgggagacg attaaatggt tataccactc ctacgtcttt
      241 tccatacggc gtataagagt gcaacatgtt gagttttccg agtgaatggt gcattttact
      301 aaccgagcca tttgaatcaa ccaacgcaat aaaaaagact ggaataataa gcctgtattt
      361 attatttatc gcaatcgcac tttacggttt tatccaactg aaaagagact gcttccagcg
      421 gttgtggatt atccctgggc gatagtcata gtctggtgtc aggattcaca aatgacctgt
      481 ttccttacat ggatataaac acaacataga gtgcggtagt taatctgaat aatctaattt
      541 cggctattca gcacaaaaca acattattga ttctttataa gagagaatct ctccatcatt
      601 tttatataaa tcattattaa gaaaattcag aagctcttgc cttacactat aaatattgac
//
LOCUS       DAZFKY010000527          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFKY010000527 DAZFKY010000000
VERSION     DAZFKY010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>658)
                     /locus_tag="ACHKCL_005233"
     CDS             complement(<1..>658)
                     /locus_tag="ACHKCL_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5765978.1"
                     /translation="STIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGESVDA
                     ALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEEEAR
                     AFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSR
                     LFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW
                     "
BASE COUNT          137 a          204 c          181 g          136 t
ORIGIN      
        1 atccatgtcc atttctgcgg catccagcca ggatacccgt cctcgctgac gtaatatccc
       61 agcgccgcac cgctgtcatt aatctgcaca ccggcacggc agttccggct gtcgctggta
      121 ttgttcgggt tgctgatgcg cttcgggctg accatccgga actgtgtccg gaacagtcgc
      181 gagggacggg tatcccaggt ggcctgaacg aacagttcac cgttaaaggc gtgcatggcc
      241 acaccttccc gaatcatcat ggtaaacgtg cgttttcgct caacgtcaat gcagcaacag
      301 tcatcttcgg caaactcttt ccatgccgct tcaacctcgc gggaaaaggc acgggcttct
      361 tcctccccga tgcccagata gcgccagctt gggcgatgac tgagccggaa aaaagacccg
      421 acgatatgat cctgatgcaa ctggatggcg ttggcggcat agccgttatt gcgtaccaga
      481 tcgtctgcgc gggcattgcc acgggtaaag ttgggcagca gggctgcatc cacactttca
      541 cccggtgggt tccacgcccg caactgccca ccaaatccgc tgccaccgcc gtgataaccg
      601 gcatattcac gcagcgatgt catgccgtcc ggccccagaa gggtgggaat ggtggaca
//
LOCUS       DAZFKY010000528          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFKY010000528 DAZFKY010000000
VERSION     DAZFKY010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>648
                     /locus_tag="ACHKCL_005234"
     CDS             <1..>648
                     /locus_tag="ACHKCL_005234"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5765979.1"
                     /translation="VRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNY
                     ALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSEN
                     VLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNID
                     EYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINT"
BASE COUNT          218 a          104 c          130 g          196 t
ORIGIN      
        1 tgtaagggat ttttttgttt ctcctggcga gtttcgtcag actggaacgg atgcatcccg
       61 cacaataaat gaaaaacaaa agccggtaat tatgttttta gttgtcgggg agacagcaag
      121 aagtcaaaac tatgctctga atggatattc tcgtggtacc aatgatttta caaagaaata
      181 taatgaactt atatcctttc ataatgtgca gtcgtgtggc acatccacag ccatatctgt
      241 tccatgcatg ttttcagata tgaagcgaaa agagttcaat tcccgcaaag cggtaaattc
      301 ggaaaatgta ctggatattc tgtacagaac gggggtaaat ttactctgga ttgaaaatga
      361 cgggggatgt aaaggtgtat gtaagcgaat ccctacaatt aatattgagc catcaaattc
      421 agataataca ttatgcaaga aaaactcatg ttatgatgag gttatgctaa aaaatattga
      481 tgagtatata aataataact cagaggataa acttatcgta tttcatttga tgggtagtca
      541 tggtcctacc tactatctcc gttatcctga gtcacataaa tattttaaac caacatgtga
      601 tcgtagtgat atcgaaaatt gtacgcatga gcaattgatt aataccta
//
LOCUS       DAZFKY010000529          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFKY010000529 DAZFKY010000000
VERSION     DAZFKY010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..288
                     /locus_tag="ACHKCL_005235"
     CDS             <1..288
                     /locus_tag="ACHKCL_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5765980.1"
                     /translation="VCVNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCKHSYAFKPC
                     DNYPIKNSGLDNETISNIHDKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            289..>640
                     /locus_tag="ACHKCL_005236"
     CDS             289..>640
                     /locus_tag="ACHKCL_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765981.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQ"
BASE COUNT          251 a           88 c          125 g          176 t
ORIGIN      
        1 gtatgtgtta atgagcacag aataaaggat attccagtag tggatcttaa tgagtttgac
       61 tcacttagta gtggtgcagt acttgtatca aaacatggct actgcaagca ctcatatgcg
      121 tttaagccgt gtgataatta tccaattaag aactctggtt tagataacga aacgatttca
      181 aatatccacg ataaaatttt aaaaagaaca ctgtatgata aaaatgatgg gaacataaat
      241 gctgataaat ggtatgaatt ccataaaaaa ataaaaaaag gagaataaat ggctaagcat
      301 ataaatggtt atcatgagaa gaagattgta gatgttatta acacatggtc tatagatgaa
      361 aaactaacct ggaatgcttt gtgcgatagg ttagtcaggg ttattgggaa aagaccatca
      421 agacaatcct taagtagtca tgtaaggatt gcggaaagct tcaatgtgaa aaaaaccaca
      481 atcaaatctg gtgtaataca cactgtaaaa cctgctaatt taaaagtggc ttctcaacga
      541 attaaaagac ttgaagcaga aaatgaagct ttgaaggcat taaatgaacg ctatttagag
      601 cagttcaaag tatggcttta taatgctcac cttaaacaaa
//
LOCUS       DAZFKY010000530          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFKY010000530 DAZFKY010000000
VERSION     DAZFKY010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.535"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..123)
                     /locus_tag="ACHKCL_005237"
     CDS             complement(<1..123)
                     /locus_tag="ACHKCL_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765982.1"
                     /translation="MIYFLTDLIKFYCLMKLYEFKNIKIDLVLTEDIIPEDKLQE"
     gene            complement(68..>640)
                     /locus_tag="ACHKCL_005238"
     CDS             complement(68..>640)
                     /locus_tag="ACHKCL_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO5765983.1"
                     /translation="NYYKLINYPIMMVHDEWLGDLTGVNQVSFRRLRETSSTRNQLNK
                     ILRQEIHDKISGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETL
                     IREIGYQNVKQLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYL
                     LPKSGEIKPTHINDLFLNRLNKILLPYETL"
BASE COUNT          210 a          122 c           81 g          227 t
ORIGIN      
        1 ttcctgtaac ttatcctctg gtatgatatc ttctgtaagc actaaatcaa tttttatatt
       61 tttaaattca taaagtttca taaggcaata aaattttatt aagtctgtta agaaataaat
      121 cattaatatg agttggtttt atttctccac ttttcggcaa aagatatttg actatggtcc
      181 ataaaggatt atcagcaaat agccactcca aatttcctgc accgtatttt attaactcag
      241 cacaaaaccg ttcttcacca tattctggtt tacattgccc tccctgccaa tagaatgata
      301 actgcttcac attttgatat cctatctctc taatcagagt ttctcgatat aatcgtgaga
      361 aaatataatc agggcattga atgtcaaata atgcactact tagagcaagc aatcttattt
      421 tcccaattga ctgatataaa aaaccttcct tatttatgtc agataattcc acaccagaaa
      481 ttttatcatg tatttcctga cgcaatatct tattgagttg attacgagta gaactagttt
      541 ctcttaaacg ccgaaaactg acctgattga caccggttaa atcacctagc cattcatcat
      601 gaaccatcat gattggataa ttaattaatt tataatagtt
//
LOCUS       DAZFKY010000531          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFKY010000531 DAZFKY010000000
VERSION     DAZFKY010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.536"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>637)
                     /gene="nleL"
                     /locus_tag="ACHKCL_005239"
     CDS             complement(<1..>637)
                     /gene="nleL"
                     /locus_tag="ACHKCL_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO5765984.1"
                     /translation="KVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQ
                     QWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMN
                     GSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLS
                     SQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSE"
BASE COUNT          222 a          142 c           88 g          185 t
ORIGIN      
        1 attcagaaaa aagattgctc aatgaatacc caaatgtatt ttgaacttct ccatccttat
       61 ataaaaaaaa ggacatccaa ttagttgatg tattaggaga taacatatca accatatgac
      121 tcaaagaaca catcattgcc ataccatcat cctgtgatga taacaaaacc cagttataag
      181 ccaaatcgga atcatcatcc cagtcaggct tcccagagcc atcgcacaat ccaaaatcac
      241 tctgctgtgt gacaggataa actttttcat tttttaaata tacttcatag agttccctaa
      301 atcgttcctg cattccatca cgccgtgaac cattcattac aatttgtata aaggaactat
      361 tataagatac catcgcacct ggatgtcgct caaaatacat ccctgcctgc aataaactac
      421 cattctccat atacatcatt attgaattat taaaccaaga cattatagta tcaaaaaagt
      481 catcacatat aggctcgagc cattgctgta tatttgcgtt gttcaaataa ggatgaatac
      541 taaatgaatc agccaaagac atcttaacat agggccggaa cgccgctaat tcgtcaagag
      601 atattttttt gataagttct tcagcggccc ttacttt
//
LOCUS       DAZFKY010000532          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFKY010000532 DAZFKY010000000
VERSION     DAZFKY010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.537"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHKCL_005240"
     CDS             complement(<1..194)
                     /locus_tag="ACHKCL_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765985.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHKCL_005241"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHKCL_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          138 a          204 c          141 g          150 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacat
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctcg ccttcgaatg cttttttgat
      601 gttaccggct cagtcgcttc tgttttcccg gtt
//
LOCUS       DAZFKY010000533          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFKY010000533 DAZFKY010000000
VERSION     DAZFKY010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.538"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>621
                     /locus_tag="ACHKCL_005242"
     CDS             <1..>621
                     /locus_tag="ACHKCL_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5765986.1"
                     /translation="ALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESD
                     RLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPE
                     TGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDR
                     LHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNG"
BASE COUNT          147 a          177 c          207 g           90 t
ORIGIN      
        1 tgcgcttgac cggctggtac agcagcgcgg ctttgacggg cggacgcaac gttaccacta
       61 tgacctgacc ggaaaactca cgcagagtga agatgagggg cttgtcaccc tctggcacta
      121 cgacgaatcg gaccgcctca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca
      181 gtacgacgag cacggctggc tgacagaaat cagccacctg agcgaaggcc atcaggtggc
      241 ggtgcattac ggttatgatg ataagggccg cctggccggg gagcgccaga cggtgcataa
      301 cccggagacg ggggaactgc tgtggcagca tgagacagag cacgcataca acgaacaggg
      361 tctggcaaac cgcgtcacgc cggacagcct gccgcgggtg gagtggctga cctacggcag
      421 cggttatctt gcggggatga agctgggcgg gacgccgctg gtggagttca cgcgcgacag
      481 gctgcaccgc gagacggtgc gcagcttcgg caataacgca tacgaactga ccagcacata
      541 cactcccgca ggccatttac agagccagcg cctgaacagc caggtgtatg accgtgacta
      601 cgactggaat gacaatggcg a
//
LOCUS       DAZFKY010000534          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFKY010000534 DAZFKY010000000
VERSION     DAZFKY010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.539"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..402)
                     /locus_tag="ACHKCL_005243"
     CDS             complement(<1..402)
                     /locus_tag="ACHKCL_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765987.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFLMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLITLIKSTDKDQQVRQVTLTR"
BASE COUNT          204 a          108 c          105 g          202 t
ORIGIN      
        1 ccttgtcaga gtcacctgcc tgacttgctg gtctttatca gtacttttaa tcaaagtaat
       61 gagtttattc aattttatat atcggctaat gaatgtttgc tctgaaagtg aaaagaagat
      121 atttctgttt ttcatctttc ttagtgaaac aatcattaaa aacctggcca aatctggcag
      181 ttttgcaagt atacttacgt catccactgc gacaagaagt acgttttttg taaagcggtt
      241 gtcaaatcta agtgtactta ggtcattatt gcatgcattt tgagaacaaa aaaaacactc
      301 aataaaaaaa cgataatggt gacctagttt tgcacttaat ccctgaaggt aatatagatc
      361 atccgaaatg ataattaccc ttgttagtaa gttcttgttc attgcactaa ctcaattgct
      421 tgcagcaaaa cagcccatct tcatggagta aaaattttga aagtgaataa agctttttac
      481 taaagatgta acaatgggca ttccatatgc gacataacaa ctgcagtaac acaactaatc
      541 tttgcgtgtt ctttatatta gagagtaaca gcgtgatatc tgtatctcta aaagaatagc
      601 gatgtcctgc aatcgatga
//
LOCUS       DAZFKY010000535          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFKY010000535 DAZFKY010000000
VERSION     DAZFKY010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.540"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            252..>619
                     /gene="tccP"
                     /locus_tag="ACHKCL_005244"
                     /gene_synonym="espF(U)"
     CDS             252..>619
                     /gene="tccP"
                     /locus_tag="ACHKCL_005244"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO5765988.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNE"
BASE COUNT          196 a          150 c          104 g          169 t
ORIGIN      
        1 ggcattttgg gaagatcagc gggacataag agacatacaa cacaggagga atggctgttt
       61 tttaatagtg ctttgaattc cgcatgaaac ctttcttagc agtcgaaaag caaaacatac
      121 gttttctttt taacaataat tgatgagtat tgcatcgagt gtcatttata catcgccgca
      181 tttatttgcc actataaaaa ggctgattac atatctatcg gagaactaaa attacttata
      241 aggtaatctg catgattaac aatgtttctt cactttttcc aaccgtcaac cgcaatatta
      301 cagctgtata taaaaaaagc agcttctctg tatcaccaca gaaaatcaca ttaaatcctg
      361 taaaaatcag ctcacctttt tcaccaagca gtagctccat cagcgcaaca actctctttc
      421 gagccccaaa cgcccattcg gcatcatttc atcgacagtc tactgctgaa agttcgttac
      481 atcaacaact tcctaatgtg aggcagcgcc tgatacaaca tcttgcagag catggcatta
      541 aacctgcccg gagtatggct gaacatattc ctccggcacc taactggcct gcgccaccac
      601 cgccagtaca aaatgaaca
//
LOCUS       DAZFKY010000536          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFKY010000536 DAZFKY010000000
VERSION     DAZFKY010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.541"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(58..327)
                     /locus_tag="ACHKCL_005245"
     CDS             complement(58..327)
                     /locus_tag="ACHKCL_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5765989.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCMVRPLQILRADGRWENIGGMK"
     gene            complement(329..>613)
                     /locus_tag="ACHKCL_005246"
     CDS             complement(329..>613)
                     /locus_tag="ACHKCL_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5765990.1"
                     /translation="GPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGA
                     LITGLTFLAPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          128 a          203 c          126 g          156 t
ORIGIN      
        1 cccccatacg tgacaacaaa aaccagagca ggactccggt ttttgtgaac ccgtcggcta
       61 tttcatcccg ccaatatttt cccacctccc gtcagcacgt aggatttgca gcggtcttac
      121 catgcactgt atctgctttt tatccgcatc cagtatcacc acctgtgtga ttaccctgtc
      181 ctgctccgga ataatgccat tctcatctga ctccaggatg tctgccggcc ccagtcgcag
      241 ttgtactgta agcgactgcc cgtgttcaca gtcatcatgc tttccgcaac cacacagacg
      301 ctccataagc tttctcaaaa tattcatgtc attctcctgt tctgcctgta tcactgccca
      361 cttcatccag ccccttaaca tcctgccacg gcccgtcacc aaacctgacc tgcaaatgct
      421 gaaaaaaacc ctgaacccgt gtggcatctt tgggggcaag aaaggtcagt ccggtgatga
      481 gtgcgccatc tgtatccggg aaccagccat tgctgtttgt ctcaataatg tttcccggcc
      541 ccagacgaaa acggatttgt gtctcccccg ggtcgccctt cggtccctga ggtccggttg
      601 cccccaccgg gcc
//
LOCUS       DAZFKY010000537          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFKY010000537 DAZFKY010000000
VERSION     DAZFKY010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.542"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>608)
                     /gene="ytfI"
                     /locus_tag="ACHKCL_005247"
     CDS             complement(<1..>608)
                     /gene="ytfI"
                     /locus_tag="ACHKCL_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO5765991.1"
                     /translation="HNNFIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWISNYDSL
                     LTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEY
                     EEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVS
                     LGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDD"
BASE COUNT          181 a          125 c           87 g          215 t
ORIGIN      
        1 atcatcaatt attttttcgt aaatatacca tccatcgcac tgtttaattc cacctcctgg
       61 cccttgtagt tcaaatttac cccgttttaa tttgcttact atatcatcca ataaagcata
      121 acctaaagac acgtcaccaa tcgcctggtg aatataacct ttaatccttt tcggtatgtc
      181 agactgtttt ttctgcagaa tatcccaggc atacaatcgt ctgttttgtt ctaacggaaa
      241 ttcacggttc ttgaaatcca tgaatgagtt atactcattt attgttatgg ggatttcttc
      301 gtattcaaat ccactgcctc gcaatgtcat tgtatattcg gcaccgttat ctttaaggct
      361 gaaactgata agaaacttat ccatagcgtt aacagtttca ttttttgatg aaagcacctg
      421 ataactactt ttatcaatct gagttgcgca acgaaagaac tccctcaaag ttaacaggct
      481 atcataattc gaaatccaat cttttttctt aaatgcatga tggatgttgt taaaaaaatt
      541 gtttgaatgt gtttttgtat acgaagactt tacaaataac tccaccgcac caataaaatt
      601 attgtgtc
//
LOCUS       DAZFKY010000538          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFKY010000538 DAZFKY010000000
VERSION     DAZFKY010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.543"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>608)
                     /locus_tag="ACHKCL_005248"
     CDS             complement(<1..>608)
                     /locus_tag="ACHKCL_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5765992.1"
                     /translation="ERLKDQETFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQ
                     LATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGA
                     GGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEA
                     IMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNG"
BASE COUNT          120 a          205 c          139 g          144 t
ORIGIN      
        1 ccattagtcc cgtcattctg aatggcgata tggtagaccg gggaatacac cagacccgca
       61 ctgccgttca tactgcccac cgcgcgcaca cccagcgagc catccgccgc ccgggtcagg
      121 ggcataatgg cttcaggtcc ggcctccccc atcagcccgg ccccttttgc aaaggcaaag
      181 tacgtgggcg tatccacaat actgttgctg tacgcactca ggtttgccga ggtatacacg
      241 ccgccttttg cattggccac cgctccgccc agccagtcac caatgctgcc gagaaatcct
      301 cccgcaccgg acataccgtt tgccgccgtc ttaattccgt tgacaatggc cgcattcata
      361 agaacttttg atatttcctg cagtacggat gaggcccagc tgcgccattc cactttgttt
      421 ccgttcagca tctccgtgat gttattcacc atccctgaga taccctccgt cgcaagctgt
      481 gctgcctgtg aggcgtaatc ggacgcatta tccacccagt tactgaatcc ctcctgcagc
      541 cctttctgcc agtccgcacg ctgcgcatcc gattcggcat aaaaagtttc ctggtcttta
      601 aggcgttc
//
LOCUS       DAZFKY010000539          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFKY010000539 DAZFKY010000000
VERSION     DAZFKY010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.544"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>587
                     /locus_tag="ACHKCL_005249"
     CDS             <1..>587
                     /locus_tag="ACHKCL_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5765993.1"
                     /translation="AASFVPVETESDPMNVAFDKLAPEVQNAVMVKFDTCENITVDMV
                     ISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQA
                     EMRIWKKRREGERKETGKYTSVVDLARARVHRQHTENSAEKIPPVTAVIRREYKQTWK
                     TLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDN"
BASE COUNT          171 a          140 c          153 g          123 t
ORIGIN      
        1 ggctgcttcc tttgtcccgg ttgagactga atccgatccg atgaacgtcg cttttgacaa
       61 gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      121 cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa cattcgacgg
      181 acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc cggagcttaa
      241 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      301 tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa
      361 atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca ctgaaaactc
      421 agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc agacatggaa
      481 aacactggat gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat
      541 tgacggcagc atccatcgct gggcaaaaaa tgaagttatc gacaacg
//
LOCUS       DAZFKY010000540          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFKY010000540 DAZFKY010000000
VERSION     DAZFKY010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.545"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..>585
                     /gene="yibB"
                     /locus_tag="ACHKCL_005250"
     CDS             3..>585
                     /gene="yibB"
                     /locus_tag="ACHKCL_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO5765994.1"
                     /translation="MTAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMI
                     IFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKN
                     PEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFD
                     ESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIG"
BASE COUNT          188 a           93 c          121 g          183 t
ORIGIN      
        1 ttatcactgc atattttgac attggtagag gggattggac tgctaataaa gggttccgtg
       61 aaaaactagc tcgttcagtt gatgtttatt ttagttactt tgagcgtctg gccgcacttg
      121 aaaatgagat gattattttc acttctcccg atttgaaacc aagggttgaa gcgattcgca
      181 acggaaaacc aacaacggtt atcgttatcg atataaaaaa gaaatttaga tatatcagaa
      241 gtcgtatcga aaaaattcaa aaagatgagt cgtttacaaa tagacttgag cctcgacaat
      301 taaaaaaccc agagtactgg tcaccagagt atgttttggt atgtaacctt aaggcctatt
      361 ttgtaaataa agctatcaac atgggcttag tgaaaacgcc tcttgttgcg tggatagatt
      421 ttggctattg ccgtaagcca aatgtaactc gaggattgaa aatttgggat ttcccatttg
      481 atgaaagtaa gatgcatctt ttcactataa aaaaagggct tactgtcaca tctcaacaac
      541 aggtttttga tttcatgatt ggcaatcatg tatatattat tgggg
//
LOCUS       DAZFKY010000541          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFKY010000541 DAZFKY010000000
VERSION     DAZFKY010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.546"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          186 a          126 c          124 g          148 t
ORIGIN      
        1 ggcggcaagc gagtgaatcc ccaggagctt acataagtaa gtgattgggg taagcgaacg
       61 cagtcgcagc acatgcaact tgaagtatga cgagtatata tatctattag ctgtttcccg
      121 gacataagct acagccagca ctaacctgga acgggtgcat tataaaaagg aacaagggac
      181 aaatagctga acagtaaccg cgttagtctc ttcccgcata accgtaccat tcgtattaag
      241 aagtattgcc catatccatt cggcgaaaga ggatctgcca tacatcaagt ccgttttact
      301 tggcatcaga tcatcgccgt cattaactct gttaaagtcg gacaaacgat tagcaagatc
      361 tgtgattcaa aagtgcttag cgctaaggtg ggactggtta cagattcatt aaccctgttc
      421 tttgtcgcac ctatcgatcg gtgtaatgcc acaacaggtt tatgttaatc agaacaatgc
      481 gcccctctat aaagagaatt ctttgataag gcaacttgaa aagggaaagc taactgatat
      541 caatgtacca ctttagtaat catcacatta tatacaggcc aaaa
//
LOCUS       DAZFKY010000542          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFKY010000542 DAZFKY010000000
VERSION     DAZFKY010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.547"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>581)
                     /locus_tag="ACHKCL_005251"
     CDS             complement(<1..>581)
                     /locus_tag="ACHKCL_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765995.1"
                     /translation="LNRIYNTKCSSCSFENSGSYMVDVYRTVKRVNETIQTPNINCYT
                     TCILTRPANGHVTSTGFSRNLMDAINISLAEHKIASLHNEVMSIEEYDRDSLQEKGNT
                     ARRYLEYILMLVNIRIMHLNNVQYQEQMLGSLVSVIEALDYEPLMKNDVEITKDILNA
                     CSHHGGVRIEKKDVIFSLEVIENLIKAIKKTDI"
BASE COUNT          174 a          103 c           89 g          215 t
ORIGIN      
        1 ttatgtcggt tttctttatg gctttaatta agttttcaat aacttccaaa gaaaaaatta
       61 catctttctt ttcaattcta acccccccgt ggtggctgca agcattaagg atgtcctttg
      121 taatttcaac atcatttttc attaatggtt catagtccag agcctcaata acagaaacta
      181 aactacctag catttgctct tggtattgaa cgttgtttaa atgcattata cgaatattaa
      241 caagcatgag aatatattct aaatatctcc tggcagtatt tcctttctct tgtaaagaat
      301 cacgatcata ttcctcaata ctcatgactt cattatgtaa agaggctatt ttgtgctctg
      361 ctaatgaaat atttatcgca tccatgaggt ttcgtgagaa gcctgtagat gtaacatgac
      421 catttgcggg ccttgttaaa atacatgtag tatagcaatt tatatttggg gtctggattg
      481 tctcatttac tcttttaacc gttctataaa cgtctaccat ataagaacct gagttttcaa
      541 atgaacatga agagcatttt gtattatata ttctatttag a
//
LOCUS       DAZFKY010000543          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFKY010000543 DAZFKY010000000
VERSION     DAZFKY010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.548"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            70..579
                     /locus_tag="ACHKCL_005252"
                     /pseudo
     CDS             70..579
                     /locus_tag="ACHKCL_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          163 a          100 c          137 g          179 t
ORIGIN      
        1 gtcatgtaag gcagatgttt gttaaagcta tttaagtctg gagtttaaat taaaataggg
       61 agttttataa tgccgttaaa ttcggagatt agatcaagct catttttaat ggattgaatg
      121 tccttcgagc tcaagtagca tctagcggtc gaggggagtt tacattaggt aatgagactg
      181 tcagcattgt atttaatgaa accgatgggc gttttctatc cagcggcagt agtgggggat
      241 tgcttactga gttattcctt tatgggttta ataacggccc tgaagctctt cgcgatagga
      301 tgctcagtat gctttcggac tcaggtgaag cacaatcgca agagagtatt caggacaaaa
      361 tatctcaatg taagtttcct gttagttcag gaaatttcca gtgcccgcca gagtctattc
      421 agtgtccaat tacactagag agacccgaag aaggagtgtt tgtcaaaaat tcagatagtt
      481 cggcagtatg ctgcttattt gattttgatg cattttctcg tttagctagt gaaggctcat
      541 atcatccact gacccgagaa ccaataacgg catcaatga
//
LOCUS       DAZFKY010000544          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFKY010000544 DAZFKY010000000
VERSION     DAZFKY010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.549"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..198
                     /locus_tag="ACHKCL_005253"
     CDS             16..198
                     /locus_tag="ACHKCL_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5765996.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            171..362
                     /locus_tag="ACHKCL_005254"
     CDS             171..362
                     /locus_tag="ACHKCL_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5765997.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            373..>568
                     /locus_tag="ACHKCL_005255"
     CDS             373..>568
                     /locus_tag="ACHKCL_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5765998.1"
                     /translation="MHFSGSRLHILCAYACRHGTCSMTPQQENALRSIARQANSEIKK
                     ARQQFPDKNVDDICRSVLKKH"
BASE COUNT          173 a          132 c          137 g          126 t
ORIGIN      
        1 tggggagcaa tggtaatgaa gcatcctcac gataatatcc gggtaggcac gatcactttc
       61 gtctactccg ttacaaagcg aggctgggta tttcccggcc tttctgttat ccgaaatcca
      121 ctgaaagcac agcggctggc tgaagagata aataataaac gaggggctgt atgcacaaag
      181 catctcctgt tgaattaaga acgagtatcg ggatggcaca tagcctcgct caaattggag
      241 tcaggtttgt gccaatacca gtagaaacag acgaagaatt tcatacgtta gccacatccc
      301 tttcacaaaa gctggaaatg atggcggcga aagcagaagc aaacgagaga gacccggcat
      361 gacaacaaca gaatgcattt ttctggcagc aggcttcata ttctgtgtgc ttatgcttgc
      421 cgacatggga cttgttcaat gacacctcag caggaaaacg cccttcgcag cattgcccgt
      481 caggctaatt ctgaaatcaa aaaagccaga cagcagtttc cggataaaaa cgtcgatgac
      541 atttgccgta gcgtactgaa gaagcacc
//
LOCUS       DAZFKY010000545          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFKY010000545 DAZFKY010000000
VERSION     DAZFKY010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.550"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>560)
                     /locus_tag="ACHKCL_005256"
     CDS             complement(<1..>560)
                     /locus_tag="ACHKCL_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO5765999.1"
                     /translation="ILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGN
                     PEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETN
                     EVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENA
                     GQPVPDEQLSSEDKYIEGEDTKSENN"
BASE COUNT          162 a          109 c           86 g          203 t
ORIGIN      
        1 attattctcg ctttttgtgt cttcaccctc aatatattta tcttctgaag agagctgttc
       61 atctggaaca ggttgtccag cattctccac atcttcaagc cacagcagaa gacgtaatat
      121 ttcatctata ttttcgaagc taacataatc gtagcgacga tgggtagcat aaatttttcg
      181 tgctaatttt ttatctgtaa taattggtat cccgatttcc tttgcatatt tccttacagc
      241 taatgctacc tcattagttt ctcttacaga aatcaatgga ataggtgaca gatgtggctt
      301 aaagtatatc cctattgcaa tgtgagtagg gttggcaatc atcaaacggc tattactgac
      361 atcagatttc aattgctcag aaagaatttc ctgatggcgc tctctgcgtt tagacttaat
      421 ttcaggattt ccttcttgtt ccttgtattc tcttttaact tcttgtttat ccattttcat
      481 atctttcata aataaaaaat attcagcgat aaaatcaaaa attaagacaa ttatcatact
      541 gccgagacaa tacagtatga
//
LOCUS       DAZFKY010000546          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFKY010000546 DAZFKY010000000
VERSION     DAZFKY010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.551"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..282
                     /locus_tag="ACHKCL_005257"
     CDS             <1..282
                     /locus_tag="ACHKCL_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5766000.1"
                     /translation="PLAFVKNLDKTLTVNTGALLTMSVSANGGTPPYKYAWKKDGQPV
                     DGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTVTVSAAAG"
     gene            297..>560
                     /locus_tag="ACHKCL_005258"
     CDS             297..>560
                     /locus_tag="ACHKCL_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5766001.1"
                     /translation="MKKDLKTLALARLSGVRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVF"
BASE COUNT          132 a          123 c          194 g          111 t
ORIGIN      
        1 ccgctggcgt ttgtgaaaaa tctggataag acacttaccg tgaataccgg tgcgctgctg
       61 acaatgtcag tcagtgccaa cgggggaacg ccgccgtata aatacgcctg gaagaaggat
      121 ggtcagccgg ttgacgggca gacgacagac accttcagta agccaggtgc gcagtccgct
      181 gatgcgggga aatatacctg tgtggtgacc gattcggcag agaaagcaca gagtgtgacg
      241 tctgttgaat gcaccgtgac agtgagcgca gccgccggat aaggggatgg gtcatcatga
      301 aaaaggatct gaaaacgctg gcgctggcca gactgtcagg ggttcgtcat aaaacggtga
      361 aggtgccgga atggggtaat gtcagcgtgg tgctgcggga gccttcggca gaggcctggt
      421 atctgtggca ggaagtgctc aatggtgatg gagaggatga cgataccctg tcggtggtgg
      481 cgaaaacccg ccgtaacctg gaagcggatg tgacgctgtt ctgcgatgtc ctgtgtgata
      541 cggacctgca gcgggtgttc
//
LOCUS       DAZFKY010000547          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFKY010000547 DAZFKY010000000
VERSION     DAZFKY010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.552"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..504)
                     /locus_tag="ACHKCL_005259"
     CDS             complement(<1..504)
                     /locus_tag="ACHKCL_005259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5766002.1"
                     /translation="MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNN
                     PALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTD
                     ISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAV
                     AEKCGYSS"
BASE COUNT          160 a          145 c          106 g          149 t
ORIGIN      
        1 gcttgaataa ccacattttt ctgctacagc ctgtccatat ccataccggg aacataacat
       61 atttacagca acacgcatcc gctcttccag caacaagcga ctgaacatgc ccccttcatt
      121 tttcagtttt gtctttaaca aactctcact catatgcaac tgtagagcaa tcgcaccaag
      181 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatgctgga
      241 taaacatcca cttaaaaaca atgacatccg ttcatctgtt tcaaacagag acaggcaggc
      301 catcataaga aacatatccg tgacctctcc ggaaaatccc tggctggtaa ttaaagccgc
      361 agccaacgca ggattgttgg gttccagcaa caggtaaagc ggaatgtcag tcagacgagt
      421 cctcgtcagc ttatgctgac tttccagata ttgacttacg acggattcgc ttatatcgac
      481 aattttaact ttgccataat gcataaggaa aagctccctg atgcatttgg tggccagaac
      541 gactgagcct ggcttaagtg
//
LOCUS       DAZFKY010000548          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFKY010000548 DAZFKY010000000
VERSION     DAZFKY010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.553"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>558
                     /locus_tag="ACHKCL_005260"
     rRNA            <1..>558
                     /locus_tag="ACHKCL_005260"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          160 a          122 c          171 g          105 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagtccctc
      481 gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacga
//
LOCUS       DAZFKY010000549          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFKY010000549 DAZFKY010000000
VERSION     DAZFKY010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.554"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            93..485
                     /gene="ydeI"
                     /locus_tag="ACHKCL_005261"
     CDS             93..485
                     /gene="ydeI"
                     /locus_tag="ACHKCL_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5766003.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          162 a          110 c          130 g          151 t
ORIGIN      
        1 gccttataac aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa
       61 taagtcaaac caaacgcaat taaggagtta gtatgaaatt tcaggccatt gtattagcaa
      121 gttttcttgt cataccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag
      181 cgccaccgcc gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa
      241 tgactgttga tttcgcaaaa accatgcacg atggcgcttc ggtttcacta cgcggtaatt
      301 tgatttctca caaaggagag gaccgttacg tttttcgcga taagagtggt gaaattaatg
      361 ttgttattcc tgcgaccgtt tttgatggac gagaagttca gccggaccag atgatcaaca
      421 ttagcggcag tctggataag aaaagcgcgc cggctgtcgt tcgggtcact catttgcaga
      481 aataaaaata aattagcctg atggcctgaa ctaccgggcc atttgggtac tgggttatgc
      541 tatttgtcat cct
//
LOCUS       DAZFKY010000550          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFKY010000550 DAZFKY010000000
VERSION     DAZFKY010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.555"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>551)
                     /locus_tag="ACHKCL_005262"
     CDS             complement(<1..>551)
                     /locus_tag="ACHKCL_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5766004.1"
                     /translation="DWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTA
                     YFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLL
                     DKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNK
                     DSRINKNVVVLAGNHEINFNGNY"
BASE COUNT          179 a          118 c           76 g          178 t
ORIGIN      
        1 tatagttacc attaaaattg atttcatgat ttccagccag aacaacgaca tttttattta
       61 tacgactatc tttattaccc tccatatttc gcatagaatt tagcaaagta aggatatatt
      121 tatcaccaaa aatagtactg aatctatcgc ctgtatgatc cccaaggaag atacaagggg
      181 taatcgcatc gccttttaaa aaaacatgtt gatgcaactc attcaagatc atgtctctct
      241 tatctaataa agtgaagagt tttttcaatt ttttattttc atatgcgaat gaagccatgt
      301 cttcgtgttt caataaatca cataataaac tccacccctt ttccctgatg cctatatgcc
      361 ctgaaacgag caatgcataa agtacagcgc ctactcttcc atctgtatcg ccaaaataag
      421 ccgtgttggc attttgtggg ttaatcacac cacactgttt ataaaagtct atttttgaaa
      481 aaacagattc aacatagcta tcaaactttt ccttagtaaa cgtctcttta gagacagccc
      541 aacgccagtc a
//
LOCUS       DAZFKY010000551          542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFKY010000551 DAZFKY010000000
VERSION     DAZFKY010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.556"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>542
                     /gene="escU"
                     /locus_tag="ACHKCL_005263"
     CDS             <1..>542
                     /gene="escU"
                     /locus_tag="ACHKCL_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO5766005.1"
                     /translation="FELLKSVFKLVIIVLIFYFMGHSYANEFANFTGLNAYQALVVVA
                     FFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERR
                     RLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIK
                     LAELYDIPVIEDIPLARTLY"
BASE COUNT          167 a           91 c          107 g          177 t
ORIGIN      
        1 tttttgagct acttaaatca gtatttaagt tagtgataat tgttctcatt ttttatttta
       61 tggggcattc atatgcaaat gagtttgcta atttcacagg actgaacgca tatcaagctc
      121 ttgtcgttgt tgcctttttt gtttttcttt tatggaaagg cgtgctattc ggatatctac
      181 tcttttcagt atttgatttc tggttccaga agcatgaggg actgaagaaa atgaaaatga
      241 gtaaagatga ggtgaaacga gaagccaagg atactgatgg taaccctgaa attaaagggg
      301 agcgccgtcg ccttcattcc gagatacaaa gtggaagttt ggctaataac atcaaaaaat
      361 caaccgttat tgttaaaaac ccgactcaca ttgcgatttg cctatactat aaacttgggg
      421 agactccatt acctttagtt attgaaacag gaaaagatgc caaagctcta cagatcatta
      481 aactggctga actctatgat attccagtga ttgaagatat tcctttagca agaactctct
      541 at
//
LOCUS       DAZFKY010000552          541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFKY010000552 DAZFKY010000000
VERSION     DAZFKY010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.557"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..147
                     /locus_tag="ACHKCL_005264"
     CDS             <1..147
                     /locus_tag="ACHKCL_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766006.1"
                     /translation="IIAHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLY
                     QLQA"
     gene            151..>541
                     /gene="ehxD"
                     /locus_tag="ACHKCL_005265"
     CDS             151..>541
                     /gene="ehxD"
                     /locus_tag="ACHKCL_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO5766007.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVP"
BASE COUNT          175 a           77 c          109 g          180 t
ORIGIN      
        1 attattgcac acaggttgtc aactgtaaaa aatgcaaaca gaataattgt tatggataat
       61 ggctttattt ctgaagatgg cacacataaa gagcttatct ccaaaaaaga cagtttatat
      121 gcatatttat atcagttgca ggcataacgt atgcgctttt acatgaaagg tttatgggat
      181 ttggtttgcc gttataagac ggttttttct gatgtctgga aaattcgtca cacacttgat
      241 gctcctgtga gagaaaaaga tgagtatgca tttcttcctg ctcaccttga actcattgaa
      301 acaccagtat ccagacgttc tcattttgtt gtatggagta ttttattatt tgtaattata
      361 tctcttctct tatctgttct tgggaaagtt gaagtggttt cagtagcaaa tggtaagttt
      421 actcatagtg gaagaagcaa agaaataaaa ccgatagaga atgcgattgt agaaaaaata
      481 atggtaaaag atggctcttt tgtaaaaaaa aatgatccat tagttgaatt gacggtgcct
      541 g
//
LOCUS       DAZFKY010000553          540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFKY010000553 DAZFKY010000000
VERSION     DAZFKY010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.558"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..399)
                     /locus_tag="ACHKCL_005266"
     CDS             complement(<1..399)
                     /locus_tag="ACHKCL_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5766008.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDD"
     gene            complement(389..>540)
                     /locus_tag="ACHKCL_005267"
     CDS             complement(389..>540)
                     /locus_tag="ACHKCL_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5766009.1"
                     /translation="RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     HNNVK"
BASE COUNT          181 a          120 c           71 g          168 t
ORIGIN      
        1 atcatcgatt atctcttcat tgtcttcaga actattcacc ttctgaatct tttcaaaaaa
       61 gctaaccaac tccggttcag aatattttat caaatatgat ttataaccac tcatgcctgt
      121 ctcaacaaaa ttaacaaccg acgctacatt attgaatgaa agattctcat tctgactgtt
      181 ataatatatt tccttcccaa ccggcatcat tacaaacatt gacaatagca aagcaacccc
      241 attaagtgtc atatttgatg gcacttgctg aagtcccaat gcgtttcgga caataacaaa
      301 aacaatcgag aatttaataa aacatgtccc ggatgctatt ataaacggca atagtgtaaa
      361 taatgatagt atcgctatca gcgaaatgct atttgacatt attatgccct gataaccaag
      421 aatgaatctc tacgcccaat ttatcatcca cttcaacaag ttcgccatag ccagttagcg
      481 cgccatttac acgtatctct atatttttct cagattcagg aagcagagat attattcttt
//
LOCUS       DAZFKY010000554          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFKY010000554 DAZFKY010000000
VERSION     DAZFKY010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.559"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(338..>535)
                     /locus_tag="ACHKCL_005268"
     CDS             complement(338..>535)
                     /locus_tag="ACHKCL_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5766010.1"
                     /translation="TTRNIRFPNQMIEQINIALDQKGSENFSAWVIESCRRELAADIK
                     YARQLTIKKNDTQYALRWLFI"
BASE COUNT          145 a          105 c          125 g          160 t
ORIGIN      
        1 ccgtattcct gcattaacag agaccgcagc ccgacaggga gactcctctg cgagagtgtg
       61 cggggataat caaaaacgat acacaccggg gtttaccgcg taaacggagc gcggcgttct
      121 cccctcatgg tcgcccgtcc ggtgcgatgg tggaagaaac tggaatctgt tcaataaaaa
      181 aactgccgtg ttggagtcac agcagtaatg tactgattgg gtagaagatt attattgtta
      241 tgctttattt ttattctata tggctgatta tttcaattcg gaattaatac agctaatgtc
      301 tgtgagtttt tataaattca gcaatataaa gaaatagtta tatgaacagc catcgcagag
      361 catactgtgt atcattcttt tttatagtca actgacgggc atattttatg tctgctgcca
      421 gctcccggcg gcaagattca atgacccacg cagaaaaatt ttctgaacct ttctggtcaa
      481 gagcgatgtt aatttgttca atcatctggt ttggaaatcg gatgttgcgg gttgt
//
LOCUS       DAZFKY010000555          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFKY010000555 DAZFKY010000000
VERSION     DAZFKY010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.560"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>530
                     /locus_tag="ACHKCL_005269"
     rRNA            <1..>530
                     /locus_tag="ACHKCL_005269"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          120 c          167 g          100 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt
//
LOCUS       DAZFKY010000556          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFKY010000556 DAZFKY010000000
VERSION     DAZFKY010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.561"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(57..380)
                     /locus_tag="ACHKCL_005270"
     CDS             complement(57..380)
                     /locus_tag="ACHKCL_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5766011.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          155 a          122 c          130 g          122 t
ORIGIN      
        1 aaacgcatta ccgctatttc tttaatcagc gaagatcccc tcgctgatta aaatatttag
       61 agaaagacag ggaaggcgga atgatggcac tgatatgaca aagtgaattc cgtattcaaa
      121 cataaggaac ccgcaaaaag gctacagttg cctactggct gaccaaaggg gatcgcgtgg
      181 gtatagcctt cctgttggca tgcgcgatcg gagatcccgc gactgacata acgatcggta
      241 tgggcatgct gcaaaaaagc ctggtcataa accaaccgta caatacctgt tgccgcgtca
      301 acttcgctca catgggcctg gcgagtaacc gtacaccccg ccagcgttat taatgaaaga
      361 gtcagaatca gaagtctcat ggtccagcca gaagagtaag agaaagcact attttacgcg
      421 ttacagcgct gcgacaatgg ccggaaaaga cgcctggcag gcgctatttc ttgccatctg
      481 taggtgtcag aaataggggg ctgttctttg attatcattt ttataacat
//
LOCUS       DAZFKY010000557          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFKY010000557 DAZFKY010000000
VERSION     DAZFKY010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.562"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            383..463
                     /gene="shoB"
                     /locus_tag="ACHKCL_005271"
     CDS             383..463
                     /gene="shoB"
                     /locus_tag="ACHKCL_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5766012.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT          151 a          105 c          111 g          154 t
ORIGIN      
        1 tggtaaggaa cgggggtttt actttttaac tttaattttg tgcagcacac gattccaacg
       61 aaaatgatta tggatcaatt tcgttgacct gtacaagcaa cttgcaatct ctgacataaa
      121 aaacgcgagc ctttacgaag aaatcgacat tgatgaaaag attaacgtga ccgctttttg
      181 ataagtacat taaaatcggt ttcgttattg gcaattttgc tgtgctttac accatgccac
      241 tgaattcccc cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcactcg
      301 attccaataa ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aacggctgca
      361 ttccttgttt aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa
      421 aatcatcatt gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt
      481 aaggtgcgtc cggtgaacca atcggacgcg cctttaataa c
//
LOCUS       DAZFKY010000558          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFKY010000558 DAZFKY010000000
VERSION     DAZFKY010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.563"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>518
                     /locus_tag="ACHKCL_005272"
     CDS             <1..>518
                     /locus_tag="ACHKCL_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO5766013.1"
                     /translation="MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARA
                     QHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGK
                     KVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIV
                     GWRVSSSMETTFV"
     misc_feature    <1..72
                     /locus_tag="ACHKCL_005272"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          117 a          122 c          162 g          117 t
ORIGIN      
        1 atgccactgc tggataagct gcgtgagcag tacggggtcg gaccggtatg cagcgaactg
       61 catattgccc cgtcaacgta ttaccattgt cagcaacagc gacatcatcc ggataaacgc
      121 agtgcccgtg cgcagcacga cgactggctg aagagagaga tacagcgcgt atacgatgaa
      181 aatcatcagg tgtacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggaatcagg
      241 gtggccagat gtacagtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg
      301 ggtaaaaagg tccgtacgac catcagccgg aaagccgttg ccgcaggcga ccgcgtaaac
      361 cgtcagttcg tggcagaacg acctgaccag ctgtgggtgg ctgattttac ttacgtcagc
      421 acatggcagg gcttcgtcta tgtggcgttt atcattgatg tgtttgccgg atacatcgtg
      481 gggtggcggg tctcatcgtc tatggaaacg acattcgt
//
LOCUS       DAZFKY010000559          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFKY010000559 DAZFKY010000000
VERSION     DAZFKY010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.564"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            262..>518
                     /locus_tag="ACHKCL_005273"
     CDS             262..>518
                     /locus_tag="ACHKCL_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766014.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGA"
BASE COUNT          147 a          100 c          159 g          112 t
ORIGIN      
        1 gtcgtggtta tcgggcggca gaggtaaaaa gaataaaaaa atcccgcaga gttgcgggga
       61 cagacaaaga ttaacgttaa ggagttattt ttgtttctgg tgctcgggca aaaaaacatt
      121 aacgcagaga aattattagc accacagtca gtttgtgaaa atgtgaagat attcagaagt
      181 tttattcagt catgatacag gcatcctccg ggatgcctgt tgtttttgtg cgtaacagtt
      241 atcacagtaa agggtgagac aatgggcaaa ggtggcggca aggcgcacac gccggtagag
      301 gcaaaggaca atcttaagtc cacgcagatg atgagcgtga ttgatgccat tggtgaaggg
      361 ccgattgaag gtccggtgaa ggggctgcag agtatcctgg tgaacaaaac cccgctgacg
      421 gacacggacg gtaatcctgt gatacatggt gtgacagcgg tctggcgcgc cggggagcag
      481 gagcagacac cacctgaagg ctttgagtcc tccggggc
//
LOCUS       DAZFKY010000560          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFKY010000560 DAZFKY010000000
VERSION     DAZFKY010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.565"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..204
                     /locus_tag="ACHKCL_005274"
     CDS             <1..204
                     /locus_tag="ACHKCL_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5766015.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     RGATYPVLIDSIAVEVNSTDTAA"
     gene            339..>517
                     /locus_tag="ACHKCL_005275"
     CDS             339..>517
                     /locus_tag="ACHKCL_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5766016.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
BASE COUNT          143 a          116 c          141 g          117 t
ORIGIN      
        1 ttccgctttg ccgggaataa cagcctgcag gtgacgccgg ttattgatgg tcaggatggc
       61 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      121 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      181 aacagcacag acactgcggc atgataaaaa aaccgccagc gacaggaatg gacgctggcg
      241 gtggtgatac ctatggagaa aaaataaagg aacgatactt tcgtactctg gtttttaatg
      301 aaaacagttc ttattgtcaa caataacgga aagaaattat gacatttctg aaccagttaa
      361 tgctgtactt ctgtacggtg gtctgtgtgc tgtatctcct ttcgggtggg tacagggcca
      421 tgcgtgactt ctggcgcaga cagattgaca aaagggccgc tgagaaaatc agcgccagtc
      481 agtcagccgg aagcaaaccc gaagagccgc tcattta
//
LOCUS       DAZFKY010000561          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFKY010000561 DAZFKY010000000
VERSION     DAZFKY010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.566"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>517
                     /locus_tag="ACHKCL_005276"
     CDS             <1..>517
                     /locus_tag="ACHKCL_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5766017.1"
                     /translation="FYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNSLD
                     EAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNPRLTV
                     SKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYK
                     TANNVYFNIIGT"
BASE COUNT          184 a           84 c           94 g          155 t
ORIGIN      
        1 tttttatatt gttaatgcca gtaatgatcc gcagctgaga catatcgata agattcttgt
       61 aaacaaaaac aggaattatg aaattttata tggtagagat catgtcatct atatcaatac
      121 caatagtttg gatgaagcag tctgggtcaa acaagcactg gaaaaaaatc aaccaggaaa
      181 gccagtacgg gtgataaatc ctgatgatga atcgatacga attttttcat ggcttgctga
      241 taatttccct gatttacaat attttaaact tcagctatta gatgccagta atcccagact
      301 gaccgtgagt aagcaacgga atgctatcac acagcaacta atcgacaatc ttattaaagg
      361 gttactacaa actatgccat atgccagcaa tataagtatt gcggtattag atgataatgt
      421 attggaaagt caggctattg aaacattgtc tgcaataggt ctttcttatg aaaaatataa
      481 aacagctaac aatgtgtatt tcaatatcat tggtacg
//
LOCUS       DAZFKY010000562          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFKY010000562 DAZFKY010000000
VERSION     DAZFKY010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.567"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..>516
                     /locus_tag="ACHKCL_005277"
     CDS             17..>516
                     /locus_tag="ACHKCL_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766018.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLL"
BASE COUNT          172 a          105 c          102 g          137 t
ORIGIN      
        1 agaaaggaca tctgaaatgt taaaccgtga cttcgatcat ctctcaacgc tattacatct
       61 cttttttgaa cgtgaggata tttgccaaaa gcttaatatt attgaccaaa acaatatcac
      121 cggttgggaa gtgtggttcc aggtagagtt tgctaatatg ctctgctcaa cagaccatga
      181 atggtggcgt gaacaagcac tttcctgtga tatgcgcaaa aaacctgagc gcccgacact
      241 acgcacagat ttcttactac gtaaaaaagg ctgggcacaa gatagctata tcgcgcttga
      301 aataaaacaa aacagagacg cgaccagttg tgttaaaaat atgatcgcag acttagaaaa
      361 aagcgcaaaa ataaaacgtt ccgaactcga tttacgcagt ttctggacct tgggtatcac
      421 ccctatgata gacagggaaa ggctggactc tctcatcgat tgctatttag atgagaaatt
      481 ttatcttact aaatctcgta aaaaacacgt tttact
//
LOCUS       DAZFKY010000563          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFKY010000563 DAZFKY010000000
VERSION     DAZFKY010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.568"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>515)
                     /locus_tag="ACHKCL_005278"
     CDS             complement(<1..>515)
                     /locus_tag="ACHKCL_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766019.1"
                     /translation="YSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEE
                     SFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDIC
                     SVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKTLLNDGCFIKYSH
                     FNDVALIKQNI"
BASE COUNT          166 a          100 c           76 g          173 t
ORIGIN      
        1 tatgttttgc tttattaatg ccacgtcatt aaaatgacta tatttaataa agcaaccatc
       61 attgagtagt gttttaagat tgattaaatt acttgcatct tcacgtacca tcgtggcaac
      121 tggaacaggc agagacatga aaacttcttc attcaccatc aagtgaatca tgcattgcgt
      181 tccctttgtc atatccacac ttacgctaca aatatcgttg taatgcgaag agtgcatttc
      241 aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat cgtgttaata aatcctgata
      301 atgaacaggt tttgtttcaa aattatcatc agcaaatcca agcactattg tatgctctac
      361 atctaacgca gtttcaaatg actcttctgt aaaatcatta tttaaaacct cacctgacgt
      421 attatcactg tcataaaaag tgctcatcaa tgcgatattg tttgttacag cgtcttttgc
      481 aatattttca ttaattgcat atcgcctgct ataat
//
LOCUS       DAZFKY010000564          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFKY010000564 DAZFKY010000000
VERSION     DAZFKY010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.569"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            377..496
                     /locus_tag="ACHKCL_005279"
                     /pseudo
     CDS             377..496
                     /locus_tag="ACHKCL_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          138 a          124 c           89 g          160 t
ORIGIN      
        1 taagttccat tttgttctcc ttaattatgt aaggagtatt tgatcatgta taagcaataa
       61 aaaacagctt caggtaatga ggaatatctc aatttttaaa cataaaatgc gaattattta
      121 gtacaaaaag tgaacttttg atgatctttc cctaatctca cataggatca gatctcgtct
      181 gatcgttccc cctgcgctca ctaccatggc tctttaccac agcagccggg gttgtttggg
      241 accgactttc gacagcagat cccggtgggc ctgccaccat ctcttcacaa gcttatgctt
      301 attacatgca aaacgtcccg ttttgcatct ttgtgcctac ggcacaccga tgatctttcc
      361 ctgtgatctt tccctgaatc tgcccctgag aacctgaatc aaacgtctga ctcgcagaac
      421 catctgtttc tcgcgctctg tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa
      481 atacatgttc tactaattga agtcatcatc a
//
LOCUS       DAZFKY010000565          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFKY010000565 DAZFKY010000000
VERSION     DAZFKY010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.570"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>508
                     /locus_tag="ACHKCL_005280"
     CDS             <1..>508
                     /locus_tag="ACHKCL_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO5766020.1"
                     /translation="CHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDL
                     ISGWEKRQVMKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQI
                     TTDTDTDKELNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQP
                     SPDFRQRAA"
BASE COUNT          145 a          116 c          151 g           96 t
ORIGIN      
        1 atgtcacggc gtgtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
       61 gcttgatgtg acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct
      121 ggatggtgat cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa
      181 tgtttcgcaa accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag
      241 actgcggaag cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg
      301 acaaatcacg acagatacag atacagataa agaattaaac cccacacata acgcgcgcat
      361 gcgcgagagt gctacgaccg gtgagttgaa tgacgcgtcg ttgcagacag ccgaacctga
      421 atacctggac ggcctgagcg aaccgatcgg gaaatttccg atgactggag tctggcaacc
      481 gtcgccggat tttcgacagc gggcagca
//
LOCUS       DAZFKY010000566          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFKY010000566 DAZFKY010000000
VERSION     DAZFKY010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.571"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..302
                     /locus_tag="ACHKCL_005281"
     CDS             <1..302
                     /locus_tag="ACHKCL_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5766021.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHKCL_005282"
     CDS             299..>504
                     /locus_tag="ACHKCL_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO5766022.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          114 a          117 c          169 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFKY010000567          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFKY010000567 DAZFKY010000000
VERSION     DAZFKY010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.572"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..504
                     /locus_tag="ACHKCL_005283"
                     /pseudo
     CDS             1..504
                     /locus_tag="ACHKCL_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
BASE COUNT          139 a          116 c          154 g           95 t
ORIGIN      
        1 atgtcacggc gagtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
       61 gcttgatgtg acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct
      121 ggatggcgat cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa
      181 tgtttcgcaa accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag
      241 actgcgggag cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg
      301 acagatacag atacagatac agataaagaa ttaaacccca cacataacgc gcgcatgcgc
      361 gagagtgctc cagccagtga gtcgaatggc gcgccgttgc agacagcaga acctgaatac
      421 ccggatggcc tgagcgaacc gatcgggaaa tttccgatga ctggtgtctg gcggccgtcg
      481 ctggattttc gacagcgggc tgca
//
LOCUS       DAZFKY010000568          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFKY010000568 DAZFKY010000000
VERSION     DAZFKY010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.573"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>499
                     /gene="espJ"
                     /locus_tag="ACHKCL_005284"
     CDS             <1..>499
                     /gene="espJ"
                     /locus_tag="ACHKCL_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO5766023.1"
                     /translation="RNADLTQEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQ
                     GHQNTVASVMDWYNPQKNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILN
                     SNDNLQKSYSKTEDSSWVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESVRVDV
                     DIYPLK"
BASE COUNT          202 a           89 c           86 g          122 t
ORIGIN      
        1 agaaatgcag atttaacgca agaaaaaaac attacagaaa gagttttatc catatttaaa
       61 ataaaacgag attttgttgc tgtaagaatc caaaacaatc agtttactga tttgaaaaac
      121 aaaaaaattc aaggccatca aaacacagtt gccagtgtca tggattggta taatccccaa
      181 aaaaatgctt tgggtataac tatgggtaca ccaagaaaat cggccgatat agcaaaagaa
      241 gagcacagaa atgcactaaa tttcatgata atggaaaaaa acacatttca tgaaaaaatc
      301 ttaaacagca atgacaattt gcaaaaatca tacagcaaaa cagaagacag tagttgggtc
      361 gcagcttctg tcggctctct tctagataaa ggagcaaaag tatatcccga tacatcatgc
      421 tctctgagac tgggagaacc attcatcttt acgctgccag aatcagttag agtcgatgtt
      481 gacatatatc ctctcaaaa
//
LOCUS       DAZFKY010000569          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFKY010000569 DAZFKY010000000
VERSION     DAZFKY010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.574"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..498
                     /locus_tag="ACHKCL_005285"
                     /pseudo
     CDS             103..498
                     /locus_tag="ACHKCL_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          146 a          122 c          118 g          112 t
ORIGIN      
        1 aaaacagagg ctttttcctc tgctgtaaat gttcgtctca ttcaaaaaac ctccgcaacc
       61 ccatgttttc acataactgt tgcgttgacc aattgaatct acaacagcgt attttgcaca
      121 ggagtcgctg aaaaatacgc gttaatcgaa caatggcgac aacaatttcc cattgaagcg
      181 atgtgtcagg tatttggtgt atccaggagc ggttattaca actgggtaca gcatgaaccc
      241 tcagacagaa aacaaagtga tgagcggcta aaactggaga ttaaggtggc acatatccgc
      301 actcgcgaaa catatggaac ccggcggctc cagacggagc tggcagagaa tggcatcatc
      361 gttggtcgtg accgactggc acgtcttcgt aaggagctaa ggctacgctg taagcagaaa
      421 cgcaagttca gggcgactac gaactcgaac cacaatctgc cagttgcgcc aaatctgctg
      481 aaccagacgt tcgctcct
//
LOCUS       DAZFKY010000570          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFKY010000570 DAZFKY010000000
VERSION     DAZFKY010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.575"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..383
                     /locus_tag="ACHKCL_005286"
     CDS             <1..383
                     /locus_tag="ACHKCL_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5766024.1"
                     /translation="SSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            395..>498
                     /locus_tag="ACHKCL_005287"
     CDS             395..>498
                     /locus_tag="ACHKCL_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO5766025.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLY"
BASE COUNT          137 a           71 c          130 g          160 t
ORIGIN      
        1 catcatcaat tgatattact ggcagtggag atctaatatc attaggtggt catgcaggac
       61 ttggcgttgc gtttgctaaa aagaaaggtg aaatgttatc tgatatttgt atttatgcta
      121 cagcatgcgg acatgcagga attggtggtg ggataaatgc ggctatcaca tattctgaga
      181 ccaagtcttt acctacatcg ggagtcagca attcagtagg tgtaacggtt ggcggcggag
      241 ttggggggca ttttgcgtat acttatgtag tggatgttga taatccagaa tcatcgacag
      301 aatctgttgg tatcggtgca ggtgttgacg cttcagttat gactctggct tgtagaacgt
      361 ggcaagaatg ctgggtcaat taacaggagg aaatatgaaa aaatatatat ttttagtttt
      421 atttttattg tatggttgtt ctccagtgga catagagcca gctagacaag aagttaatat
      481 ctttcgtggt ttatatca
//
LOCUS       DAZFKY010000571          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFKY010000571 DAZFKY010000000
VERSION     DAZFKY010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.576"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          149 a          102 c          116 g          130 t
ORIGIN      
        1 cagcgggtct ggacagaaag cggctcttga tgtggattat cgcatggtgt gggctttcag
       61 cggcatggtg actggaaagt aacgatattg catctgtggc aacgaaaatt gcagagttgg
      121 tactctttga actgcataag taagcagaaa ggtaaggtta tgctttgcga ttgaaattca
      181 gccttaacgt acaataattt tcgatatcca aactgccccc ctataacagc aaaaggtccc
      241 acaagacgct cgtggaatat attttaaaag agaacaaaaa ttgcaatatg tggtggctcc
      301 atacagagga ttgacaacat tcccatgcta attgttaaga ttcaaattga agtatatcta
      361 tctgtcattt ttgcaaatga catgcggtag cataacgttc cgggggctcc tgtgccgtca
      421 tgcgtgacga cattgatcag ccaataatgg cacgtgacca cagccagata cggccagcct
      481 tcatccgtac gtaaata
//
LOCUS       DAZFKY010000572          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFKY010000572 DAZFKY010000000
VERSION     DAZFKY010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.577"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          133 a          103 c          125 g          129 t
ORIGIN      
        1 agatcgcaga aaataaaaaa acctgctggt gaggcaggtt cttttattta ctacagtgac
       61 gctaacttgc tgattgtgca acatttcgta ccaacgaata aattataacc atatccacac
      121 tccattgcaa ttcagtgagt gagatttata atcctgcccg ttgggaggtt gtgccggatc
      181 ggaccaattt taagtcctgg ctgccggacg ggtggtgccg caggcggtgc cctgatccgg
      241 agagcaacgt ttcgtaccaa catacgtaaa ccagtatcga caggcagtgt aagggtaagg
      301 tgctggtgtt gcgctcctta ataaggagag cggatggaca cgctgacaca aaagttaacc
      361 gtgctcattg ccgtactgga gttattagtg gctctgttac ggttgattga tttgttgaag
      421 taacgggcaa attgaaacgc atctaagggg gaataccgtt tcccccttta agaaagctat
      481 actcgtcata
//
LOCUS       DAZFKY010000573          489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFKY010000573 DAZFKY010000000
VERSION     DAZFKY010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.578"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(371..>489)
                     /locus_tag="ACHKCL_005288"
     CDS             complement(371..>489)
                     /locus_tag="ACHKCL_005288"
                     /inference="COORDINATES: protein motif:HMM:NF025345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766026.1"
                     /translation="QGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          192 a          108 c           58 g          131 t
ORIGIN      
        1 gcaaaacaaa atcaattatc acattcaaaa aaatactgac aacaaagata ctcttcagac
       61 aaagttgtca tatgtaaatt ttcccttaaa acgttgctca agaaaaatat aaaaatcata
      121 tgccctaatc aacatcctca agcactacga tatatgcagt atcctattca tatattacca
      181 ccatatcaga caaatcgaat cacaacgagt tctatcgtac tctttcatct tcgacactgt
      241 aaattttaag aacattcggc tcatgccact aaaccaagac ataataacaa cggagcaata
      301 aatgttccca ctgaacatgt gcaaacctaa aaagacagtg ttacaataat attcatccat
      361 ggtctctaaa tcaacgccac gcaacaggat aatacaatga aaatatttca ggaaaagatt
      421 cttttgcatg ctccgttagc atatcagtca gcacagcggt acaagtaaaa acattatttc
      481 ttccttgta
//
LOCUS       DAZFKY010000574          488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFKY010000574 DAZFKY010000000
VERSION     DAZFKY010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.579"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>488
                     /locus_tag="ACHKCL_005289"
     CDS             <1..>488
                     /locus_tag="ACHKCL_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5766027.1"
                     /translation="TEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTE
                     RELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKPKSR
                     GGKSETEKAEDSFSRLLKQQKAQLALAGQNTELAKLKYQTAQGELKTLTEIQKQELLR
                     NA"
BASE COUNT          136 a          109 c          161 g           82 t
ORIGIN      
        1 atacggaatt caaccgtctg ctggggctgg gtaatgaact gcttcagcag cggcagggac
       61 tggtgaatgt gccgttacgg ctgccacagg ccactctgga tgataaacag cagagtgccc
      121 tgacaaaaac agagcgtgag ctggccctgt ccagactgaa aggggaagaa aaagagcgtg
      181 cccggctggg gtatgcggcg gatgaccttg gttttgtggg ggattcgtat caggaggcga
      241 gacagcgtta tatcagtaat gctctggaag cctggcgtaa taacgaggcg aacaaaccta
      301 aatcccgggg tggaaaatca gagacggaaa aagcggaaga cagtttttcc cggctgctga
      361 agcagcagaa ggcgcaactg gcactggccg gtcagaatac agagctggcg aagctgaagt
      421 accagactgc gcagggcgaa ctgaaaaccc tgacggagat acagaagcag gagctgctgc
      481 gcaatgcg
//
LOCUS       DAZFKY010000575          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFKY010000575 DAZFKY010000000
VERSION     DAZFKY010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.580"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>483
                     /gene="nleH1-2"
                     /locus_tag="ACHKCL_005290"
     CDS             <1..>483
                     /gene="nleH1-2"
                     /locus_tag="ACHKCL_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO5766028.1"
                     /translation="SVRDAAVHSDSGTQVTVGNRTYRVVVTDNKFCVTRESHSGCFTN
                     LLHRLGWPKGEISRKIEAMLNTSPVSTTIERGSVHSNRPDLPPVDYAQPELPPADYTQ
                     SELPRVSNNKSPVPGNVIGKGGNAVVYEDMEDTTKVLKMFTISQSHEEVTSEVRCFNQ
                     "
BASE COUNT          143 a           84 c          126 g          130 t
ORIGIN      
        1 cctctgtaag ggatgctgct gttcactctg atagcgggac gcaagtaacg gttggcaaca
       61 gaacatatcg tgttgtggtc actgataata agttttgcgt tacaagagaa agtcatagtg
      121 gttgttttac taatctgttg cacaggttgg gatggcctaa gggagagatt agcagaaaaa
      181 ttgaggctat gctgaataca tcgccagtga gcacgactat agaaagaggc tctgttcatt
      241 cgaacagacc tgatttacct ccagtggatt atgcgcagcc ggagttacct ccagcggatt
      301 atactcaatc agagttgccg agggttagca acaataaatc acccgtgcca ggtaatgtta
      361 ttggtaaagg tggtaatgct gtcgtgtatg aagatatgga agatacaaca aaagtgttga
      421 agatgtttac tatatctcaa agccatgaag aggtgacaag cgaagttcgt tgtttcaatc
      481 agt
//
LOCUS       DAZFKY010000576          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFKY010000576 DAZFKY010000000
VERSION     DAZFKY010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.581"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>481
                     /locus_tag="ACHKCL_005291"
     CDS             <1..>481
                     /locus_tag="ACHKCL_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO5766029.1"
                     /translation="NELKMESETARKEYQLFRQGNYDKYITTDIFEEHGLIKYVNLSF
                     DIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLSPLFFNEYDSGP
                     IRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNRLIRPSLGNRPG
                     "
BASE COUNT          164 a           66 c          104 g          147 t
ORIGIN      
        1 tcaatgaatt aaaaatggag tcagaaacag caagaaaaga atatcaactt ttcagacaag
       61 gaaattatga taaatatatc actactgata ttttcgaaga acatggtttg ataaaatatg
      121 tgaacttaag tttcgatatt gttgcatctg taggacaggt cgtaggcggg gttggcgcgt
      181 tgaaatttgg taaggtagtg cattcaaacc gaataaaagg tataggcgtc acattagttg
      241 ctcatggggc gaataatttt tatgaaagct tatcaccttt attttttaac gagtatgatt
      301 caggtccaat acgtgaactc tacagagtaa tagcaaaaaa aatgggaggt gatattaatt
      361 ctgctgatta tgcatacagc atcgtggact tttcgattac tgcatatggt ggatattcag
      421 gcatgaaaat cgtacctaag tataatagat tgattcgtcc ttcattagga aatagacctg
      481 g
//
LOCUS       DAZFKY010000577          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFKY010000577 DAZFKY010000000
VERSION     DAZFKY010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.582"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            158..>474
                     /gene="grlR"
                     /locus_tag="ACHKCL_005292"
     CDS             158..>474
                     /gene="grlR"
                     /locus_tag="ACHKCL_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO5766030.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAK"
BASE COUNT          166 a           62 c           99 g          147 t
ORIGIN      
        1 atatgttaac cagggaaaca gcaggttgaa acatgagtat atttaatgat atattacatt
       61 gcaatctgga gaaaaagaaa ggtctccatt attcttgata ttgcttatgg atagaacaaa
      121 ttgaaaggag tgaggttagt atgaaactga gtgagttatg attatgaagg atggcatcta
      181 tagcattata tttattagca atgaagactc ctgtggggaa ggtatactga ttaaaaatgg
      241 aaatatgatc actggcggcg atattgcttc tgtgtatcag ggggtcctct ctgaagatga
      301 ggacatcata cttcatgtcc atcgatataa ttacgaaatt ccctcggtgc taaacattga
      361 acaagattat caattagtta tccctaaaaa agtactgagt aatgataata atctcacatt
      421 acattgccat gtaagaggaa atgaaaaatt gtttgttgat gtttatgcca aatt
//
LOCUS       DAZFKY010000578          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFKY010000578 DAZFKY010000000
VERSION     DAZFKY010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.583"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>471
                     /locus_tag="ACHKCL_005293"
     CDS             <1..>471
                     /locus_tag="ACHKCL_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO5766031.1"
                     /translation="ISEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRG
                     ALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKL
                     ALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNILKT"
BASE COUNT          138 a           85 c          104 g          144 t
ORIGIN      
        1 catttcagag gtaattcagc gtctgatcca ggagcgaatt tcagtgcgca atatgcggtt
       61 agttatggag gccttagcat tatggagccc acgagagaaa gatataatca cactcgtcga
      121 gcatgtacga ggtgcattag gtcgctacat ttgccataag ttttcatatt ctggagaaat
      181 taaagcaatt gtgatttcgc ctgaaataga ggacagaatc agagatggtg tcaggcctac
      241 cgctggcggt acttttctta atctcgatgc atccgaagct gagatgattc tggataattt
      301 taaactggca ctttctggaa tcaatattcc tattaaagac attattttac tgggatctgt
      361 ggatattcgt cgttttatca aaaaacttat tgaatctagt tatcgagacc tcgaagtact
      421 ttcgtacggg gaactaacgg aaaatgttcc cgttaatatt ctgaaaacta t
//
LOCUS       DAZFKY010000579          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFKY010000579 DAZFKY010000000
VERSION     DAZFKY010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.584"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..125
                     /locus_tag="ACHKCL_005294"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACHKCL_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(320..433)
                     /locus_tag="ACHKCL_005295"
     CDS             complement(320..433)
                     /locus_tag="ACHKCL_005295"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5766032.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          148 a          124 c          104 g           93 t
ORIGIN      
        1 cgacatcgaa tttctcccgt gtagaggaac agagttaaaa agccggaagc ggaatcaaat
       61 cacaggatga ccatctgcca gtggctggtc gtaaaaaaag gccgcgccat gcgcagccga
      121 aaataaaggg ataacgatga tagtttgaga aaaacagaaa taacactttt gtggcaaagc
      181 atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg attaccccat
      241 attccttctt gctgattgcc ccaccgcaca gggggattca ccatgcagaa gtgtttttaa
      301 taaacagcaa acaaaaaaat caagcattat gcaggctgtt tctttttatc accggccaca
      361 gcaataccat aatgccgcag accagcaccc catccgccag caccgacatg attctgctgg
      421 tgaaatccac catcaccacc agaaacagca ggagtgcagc cacagtcag
//
LOCUS       DAZFKY010000580          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFKY010000580 DAZFKY010000000
VERSION     DAZFKY010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.585"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>469
                     /locus_tag="ACHKCL_005296"
     CDS             <1..>469
                     /locus_tag="ACHKCL_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5766033.1"
                     /translation="LQTLIESIELLLDEALETGENESTEFDVTTLLRMDSERRMKTLG
                     ESVKNTLLTPNEARKRENLPPLAGGDALYLQQQNYSLEALSRRDAREDPFASAGKTVS
                     AQLPDGASDGNKAISETEHDAVKAMFRGDTEKMTERELSIIRALGEEFSTVLAD"
BASE COUNT          122 a          111 c          146 g           90 t
ORIGIN      
        1 ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga aacgggggaa
       61 aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga acggcgcatg
      121 aaaacactgg gtgaatcggt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgt
      181 gagaacctgc cgccccttgc cggcggtgat gcactgtatc ttcagcagca gaactacagt
      241 ctggaggcgc tgtcccgtcg tgatgcccgt gaggacccgt tcgcgtcggc cgggaaaaca
      301 gtttcagcac aactgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag
      361 catgatgcgg tgaaagcgat gttcaggggg gatactgaga aaatgacgga acgggaactg
      421 tccattattc gtgcactggg agaagaattt tccacagtgc tggcggatt
//
LOCUS       DAZFKY010000581          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFKY010000581 DAZFKY010000000
VERSION     DAZFKY010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.586"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(118..420)
                     /gene="bamE"
                     /locus_tag="ACHKCL_005297"
     CDS             complement(118..420)
                     /gene="bamE"
                     /locus_tag="ACHKCL_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5766034.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          122 a           94 c          100 g          152 t
ORIGIN      
        1 tgggggaggc tgaaaaagat aatcactttc tttaaaaagt gatttatgtt tctgtaaaaa
       61 gcccgtaggt atcaactgcg ggctaaattt tcccttgatg ctggttttgt cttttatcta
      121 aaaaatgcca gtcttcacgt tgcttgttcc agcattaaag atgtatgtgc tgactttttc
      181 gcctttgaaa gaaactgtca gagatttagt ttgtgagtct gcgccacctg caagcagtcc
      241 cacaacgggg atgaaagagg ttgctttgga ctggctgtta tacatagtgt atgaccattg
      301 ttcttcacca tcgatcaaag aacggctgtc aggttcaccg aacgatgcta acacgtcctg
      361 ttttgtagtt ttgcctttaa caattttggt tttcacactt tcctgcgtct catttttcaa
      421 tgactggttg cctactgaag aacaacctgc caaggtcaga aaaacgaa
//
LOCUS       DAZFKY010000582          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFKY010000582 DAZFKY010000000
VERSION     DAZFKY010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.587"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..449)
                     /locus_tag="ACHKCL_005298"
     CDS             complement(<1..449)
                     /locus_tag="ACHKCL_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5766035.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAARDQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDV"
BASE COUNT          108 a          140 c          116 g          100 t
ORIGIN      
        1 acatcatcag cactgtaacc atattcacgc attgaggctg ccgagcgggc aaacagggcc
       61 gcattatccg aaaaagcggt acctgtccgc tgactgatat ccatcagcac tttctgtgat
      121 gacgaaaatt catcagatga ctgcgacgcc tgtttcagac gggcattcac ggaactccac
      181 tcatcagcca gtgaaatcag gtgtccggtg gcaaaggcac cggcaaatgc gccagccatt
      241 ccgacagcag aaccgcgaat ttccgtcaac tggctgttca gttctgccag ggcacgtcgc
      301 tgatcccggg ctgccgcagc ggcctgacgc ccgccattct gcagggtccg gtaatattca
      361 ctgcccatac gggacgcccg ctggatctcc gactggaatg actgcgaatt tgccgaaatt
      421 ttgataatca gttcacgtaa cgtcgccatt cacctttctc cggg
//
LOCUS       DAZFKY010000583          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFKY010000583 DAZFKY010000000
VERSION     DAZFKY010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.588"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>464
                     /locus_tag="ACHKCL_005299"
     CDS             <1..>464
                     /locus_tag="ACHKCL_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5766036.1"
                     /translation="MADDGQLTADKVVPALISQLEVLRDEYAAMPETVSDGITKVENA
                     FMAWVGGANEASGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSA
                     TAGLVTAARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAA"
BASE COUNT           97 a          111 c          170 g           86 t
ORIGIN      
        1 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
       61 gaggtattgc gtgatgaata cgcggccatg ccggaaacgg tctctgacgg gatcacaaag
      121 gtggaaaacg cctttatggc ctgggtgggt ggtgcgaatg aggccagcgg ggtgacgaaa
      181 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagccgtg
      241 ggggcactgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      301 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      361 ttcaggggaa cgcagattgc cacggcgcgg gcaagagcag ccgtgtaccg tgctcagcag
      421 gccgtggcgg cagcccgcgg gacggagatg cagattgctg caga
//
LOCUS       DAZFKY010000584          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFKY010000584 DAZFKY010000000
VERSION     DAZFKY010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.589"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>461)
                     /locus_tag="ACHKCL_005300"
     CDS             complement(<1..>461)
                     /locus_tag="ACHKCL_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5766037.1"
                     /translation="EITATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHE
                     YTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASS
                     AGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTL"
BASE COUNT           91 a          137 c          127 g          106 t
ORIGIN      
        1 agcgtgcgcc cttcctctgc ggctttggtc atgacctcct caagggaggc tgccagcgct
       61 gcacgcttgt tttcaaaact tttaatctgt tcgccaatat tcattatggt cttttcctta
      121 tgaaaaacgg ttgttgactg tgccgcagcg ccggcagaag atgcgatttt caccaccggt
      181 ttccggttgc cggacgcggc agaaaacggg cggtcgtaag atttaatggt ccggatggtg
      241 cattccgcat tcgcgggcac ggtgacggca gacacctcca tcagttccca gcgcagaaaa
      301 tgcagtccgc ctccgtccag aaaggtgtat tcatggggac ggaagcccac ggacagcccc
      361 ctgaccagcc cggtcttaat ggccgcccag acctcatcca gccgggcagc cagttgcgac
      421 ggcatatccg gtacgggctt caccagtgtt gccgtgattt c
//
LOCUS       DAZFKY010000585          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFKY010000585 DAZFKY010000000
VERSION     DAZFKY010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.590"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>455)
                     /locus_tag="ACHKCL_005301"
     CDS             complement(<1..>455)
                     /locus_tag="ACHKCL_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766038.1"
                     /translation="HSILSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAMQDFLN
                     DINFENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSRKGNPF
                     IGFVVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVI"
BASE COUNT          147 a           91 c           67 g          150 t
ORIGIN      
        1 aattacaatc tggaaattat agtccctttt gattaatgcg tgataaaatg aatcactgag
       61 actgacgcta ttaacatccg atagagaacc ccctctatct ttatcaaagt gcgtcactaa
      121 tggactatca agcacaacaa atccaatgaa tggattccct tttcttgata aaaattccgc
      181 aaagcaaatg gtaaatatag catgcattac tgcacgggta cctttaccaa acattcctcg
      241 gcgtttccca ttgatattaa agtcatatgt atcttcgtca aatcgcactt tgttatcttt
      301 agaattttca aaatttatat cattcaaaaa gtcttgcatc gctatttcta tatcatacaa
      361 atcagctact gttaaggagt caaagtcctc ggcggagtat ttaagttcgt caacatcatc
      421 ttctttatct tgtaatgaag atagaatgct atgtt
//
LOCUS       DAZFKY010000586          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFKY010000586 DAZFKY010000000
VERSION     DAZFKY010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.591"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>455)
                     /locus_tag="ACHKCL_005302"
     CDS             complement(<1..>455)
                     /locus_tag="ACHKCL_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5766039.1"
                     /translation="KSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLI
                     ELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIV
                     KESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNIND"
BASE COUNT          139 a          105 c           58 g          153 t
ORIGIN      
        1 ttatcattaa tgtttttttc tatatgcttc aaagcctcat attttatctg cagcgaacgg
       61 ctatgtaagt cagagcgagt gcctggaaca aagtccattc caatacgtac agccccctga
      121 atataatcac agatattgct agattctttt actatagcac tatgtaaaat gctacttaca
      181 ccagacaatg cattataacc aggtacagct cccatcgcca tattccaaat tgtacctaaa
      241 aaatgtaaca cagctctccc atcaggagaa agagaagact tatcacgatg atttgtacta
      301 tcattaatat cgatacttaa ctcaatcaat ccacttaagc cttcttgaat aaccttatca
      361 aacccccaaa taaactgctg cccgccattc atatgggata cttctttttt gaacttttct
      421 attgcctctt tttgtttatt attcttttct gattt
//
LOCUS       DAZFKY010000587          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFKY010000587 DAZFKY010000000
VERSION     DAZFKY010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.592"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..351)
                     /locus_tag="ACHKCL_005303"
                     /pseudo
     CDS             complement(1..351)
                     /locus_tag="ACHKCL_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          150 a           88 c           67 g          145 t
ORIGIN      
        1 tttttctttt acagcaaatt tgagacctaa aacttctatt tgctgattca atgttaacgt
       61 tctatcttca ggttcttgtg ctgttaattc tctgattgtt acaaccagat cagggtcggt
      121 agtgattata atctcaaaac tacctgtaag ctcgccataa ggaacgacta ttgtattttc
      181 tggtaactca gaaaaagcat cacttcttcc taacgaaaca tcattaccaa tgataacaag
      241 tagacgccca ttaagtattt catactcgca tccattctat ggtccgctca acaaacgaat
      301 ggcataagat tccaataagt cttgtgataa gaatttatca ttattttcca tgaataccac
      361 ccgctaattc cataagctct agtttagagt agtgtaacaa gacggaaatt tcatgaccaa
      421 attatgatga gacagaataa aaaaattata
//
LOCUS       DAZFKY010000588          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFKY010000588 DAZFKY010000000
VERSION     DAZFKY010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.593"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..362
                     /locus_tag="ACHKCL_005304"
     CDS             <1..362
                     /locus_tag="ACHKCL_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766040.1"
                     /translation="GKDGIANMSLSIYAYSTCRFWSKESVAVGDTIKLSNYEHIEFSF
                     NSFSKDYFREQNSDIYKIFFKIASSSNSVVVDKNTLTQKNFLENNTFFSITGIDESLY
                     EKHIIFITLKVIVPSKK"
BASE COUNT          147 a           76 c           76 g          145 t
ORIGIN      
        1 ctggaaaaga cggtattgcc aatatgtcat tatctatata cgcatactcg acatgtcgtt
       61 tctggtcaaa agaaagtgtg gctgtaggag atacaataaa attgtctaat tatgagcata
      121 ttgagtttag ctttaattca tttagtaagg attactttcg tgaacaaaat agtgatatat
      181 ataaaatatt ttttaaaatt gcttcttcga gtaattcggt tgttgtggat aaaaatacgc
      241 tcactcaaaa aaacttccta gaaaacaata catttttcag tattacaggt atagatgagt
      301 ctctctatga aaagcatata atctttatca ctcttaaggt tattgtcccc tcaaaaaagt
      361 agcgctgatt tcctgtatgc ggaatttgct gactaatctt acccagcatc agcagacgtt
      421 ccaaaggcca cgacatactt cgcg
//
LOCUS       DAZFKY010000589          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFKY010000589 DAZFKY010000000
VERSION     DAZFKY010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.594"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHKCL_005305"
     CDS             complement(<1..203)
                     /locus_tag="ACHKCL_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5766041.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>437)
                     /locus_tag="ACHKCL_005306"
     CDS             complement(355..>437)
                     /locus_tag="ACHKCL_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5766042.1"
                     /translation="IDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          118 a          123 c           88 g          108 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatgg
//
LOCUS       DAZFKY010000590          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFKY010000590 DAZFKY010000000
VERSION     DAZFKY010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.595"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>437)
                     /locus_tag="ACHKCL_005307"
     rRNA            complement(<1..>437)
                     /locus_tag="ACHKCL_005307"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          101 a          131 c           99 g          106 t
ORIGIN      
        1 ttcgtgctcc tccgttactc tttaggagga gaccgcccca gtcaaactac ccaccagaca
       61 ctgtccgcaa cccggatcac gggtccacgt tagaacatca aacattaaag ggtggtattt
      121 caaggtcggc tccatgcaga ctggcgtcca cacttcaaag cctcccacct atcctacaca
      181 tcaaggctca atgttcagtg tcaagctata gtaaaggttc acggggtctt tccgtcttgc
      241 cgcgggtaca ctgcatcttc acagcgagtt caatttcact gagtctcggg tggagacagc
      301 ctggccatca ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa tttcgctacc
      361 ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag cttcgcttgc
      421 gctgacccca tcaatta
//
LOCUS       DAZFKY010000591          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFKY010000591 DAZFKY010000000
VERSION     DAZFKY010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.596"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..339)
                     /locus_tag="ACHKCL_005308"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKCL_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          104 a          109 c          100 g          116 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaa
//
LOCUS       DAZFKY010000592          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFKY010000592 DAZFKY010000000
VERSION     DAZFKY010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.597"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          153 a           73 c           66 g          137 t
ORIGIN      
        1 tgagtattaa aatttaattg atattgtcta taaaatgtag cgatgcaatt ttattgcaac
       61 gaaatgattt ttacaacata ccattatcgc taaacaaaat cgcacccatc aaatcaactt
      121 acgttacaat cactacaaag cgattgcatt taaattcaaa acagttttct gttgggcgca
      181 ttttttgttg tactggacta aagagaaaaa atggggtaag gaacaaatac cttccgtgct
      241 caaaaattag gtttttatgc acttattaat cagtcaaggt gaacagtaaa gtgttcactt
      301 cttatcaatc gttaatttca tatctttttg aatattaatg aaaaaaaaca caatgagtag
      361 tatgaacagt tttcccccaa aaaaagtttt ttctgccttg agaatgcgcg gaaagcgaac
      421 gccttcacc
//
LOCUS       DAZFKY010000593          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFKY010000593 DAZFKY010000000
VERSION     DAZFKY010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.598"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>425
                     /locus_tag="ACHKCL_005309"
     CDS             <1..>425
                     /locus_tag="ACHKCL_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO5766043.1"
                     /translation="LKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKECSS"
BASE COUNT          165 a           49 c           94 g          117 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatgg catggatcca
       61 ttaggattat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      121 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      181 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      241 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      301 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tagtctaaat
      361 ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa agaatgcagt
      421 agtta
//
LOCUS       DAZFKY010000594          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFKY010000594 DAZFKY010000000
VERSION     DAZFKY010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.599"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>425
                     /locus_tag="ACHKCL_005310"
     CDS             <1..>425
                     /locus_tag="ACHKCL_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5766044.1"
                     /translation="EKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGP
                     AGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKA
                     VKAAMDVANEAKTKAEEAAAGGGVPGPKGDKGDTGPAGPA"
BASE COUNT          118 a           98 c          163 g           46 t
ORIGIN      
        1 gagaaaggtg atccgggagg tcctgcaggc ccgcaaggtc ctaaaggcga acgaggagaa
       61 gccggaccac agggaccgat gggagcacga ggtgagcgtg gggagactgg cccccgaggt
      121 gaacctggtc ctgcaggtcc gagaggcgaa cgaggagaga ccggacctca gggacctcgt
      181 ggagagccag gtccggcagg cagcgctgca aatgtggctg atgcaacgac ggcacagaag
      241 ggaattgtgc agttaagcag cgcaacggac agtgatgatg aaacgaaggc tgccaccccg
      301 aaagcggtga aagcggcaat ggatgtggca aatgaagcga aaacaaaggc agaagaggct
      361 gcagcaggag gtggtgttcc cggtccgaaa ggagataaag gggacacggg gccagcaggt
      421 ccggc
//
LOCUS       DAZFKY010000595          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFKY010000595 DAZFKY010000000
VERSION     DAZFKY010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.600"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..410
                     /locus_tag="ACHKCL_005311"
     CDS             <1..410
                     /locus_tag="ACHKCL_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766045.1"
                     /translation="PSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILSRVYDFLLP
                     AQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISERLIREADT
                     REYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          120 a           79 c           81 g          143 t
ORIGIN      
        1 caccttcatt aagaaagaaa aattttactg acgcatataa tgcagcatct tctcatatcg
       61 aatactggat aggtgctggc gtctcattta tctcgatgat gattttatcc agagtatatg
      121 actttctttt acccgcccaa gatactttcc cgggtgatat catcagaagt ttatgtgttg
      181 tgtgtccatc catattaatc tggtttcagt tttcggtata tgtcatgagg aaatattaca
      241 gacatattct ggtgcgagga aaagaaaccg aaaccatttc agaaaggttg atacgtgagg
      301 ctgatacacg agaatatgaa ctatggcgtc cggtccgtcg ctttttctcc attgttttcc
      361 tcattgttct attaggttgc attcattcac tgataacaac tataaaataa aagttatggc
      421 tgt
//
LOCUS       DAZFKY010000596          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFKY010000596 DAZFKY010000000
VERSION     DAZFKY010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.601"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>423
                     /locus_tag="ACHKCL_005312"
     CDS             <1..>423
                     /locus_tag="ACHKCL_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5766046.1"
                     /translation="NKLTSLLTSEEVILTYPAGKCLKLLLDNAYSVVSHNQFFEKVWS
                     DSSADVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGFKINEEISITVLDNNH
                     NENESVESEENGTHTENINKNHIAKGIFTTKNTLLIVC"
BASE COUNT          156 a           75 c           74 g          118 t
ORIGIN      
        1 ctaataagtt aacatcactc cttacatcag aggaagtgat actcacttat cctgcgggaa
       61 aatgcttaaa gttactttta gataacgctt atagtgtcgt gtcacacaat caattctttg
      121 aaaaagtctg gagcgatagc agtgccgatg tcgcaaccaa cactttatat caaaatatat
      181 cgatgataag acgttctttt cgtgaaatca gctcagcgac gatatttaat gatgttatca
      241 gaacggtgcc ccgtaaaggc ttcaaaatca atgaagaaat cagcataact gttctcgaca
      301 ataatcacaa tgagaatgaa agtgttgaga gtgaagagaa tggtacccac actgaaaata
      361 taaataaaaa tcacattgca aaaggtatat ttaccacaaa gaatacatta cttatagttt
      421 gtt
//
LOCUS       DAZFKY010000597          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFKY010000597 DAZFKY010000000
VERSION     DAZFKY010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.602"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            10..>423
                     /locus_tag="ACHKCL_005313"
     CDS             10..>423
                     /locus_tag="ACHKCL_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5766047.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLN"
BASE COUNT          142 a           81 c           64 g          136 t
ORIGIN      
        1 ggttggcata tgcaagtctt ttccagtgat gtctacttca ctgtaggcac taatgcatta
       61 ttagcatccc aaaaggaata ttacagcgat ttagttgcgt tggttgatct tggacattcg
      121 ttcgttgtta ttgatgagca tcaacatcga aatctcaaac caaatacaga acctgtaaac
      181 atccttttga gtaacaattt catctggatc aataagaata ttacattatc tgatttaacg
      241 catttcctgg tttcaaatct tcatacacaa aatgtgtatt ccacacaaga accacttacc
      301 catgatgaga tagatatcct gcggttatgt gtttcatatt cattaaagca aattgccatt
      361 attaaaggaa ttgattataa aacagtatca tatcacaaaa taagagccct caatcaactt
      421 aac
//
LOCUS       DAZFKY010000598          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFKY010000598 DAZFKY010000000
VERSION     DAZFKY010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.603"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>421
                     /gene="wzx"
                     /locus_tag="ACHKCL_005314"
     CDS             <1..>421
                     /gene="wzx"
                     /locus_tag="ACHKCL_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO5766048.1"
                     /translation="VAVFSITQRLFQISTVPLTIYNIPLWAAYADAHARNDTQFIKKT
                     LRTSLKIVGISSFLLAFILVVFGSEVVNIWTEGKIQVPRTFIIAYALWSVIDAFSNTF
                     ASFLNGLNIVKQQMLAVVTLILIAIPAKYIIVSHFGLT"
BASE COUNT          127 a           70 c           75 g          149 t
ORIGIN      
        1 atgttgctgt ttttagcatt acacagagat tatttcaaat atctacggtc cctcttacga
       61 tttataacat cccgttatgg gctgcttatg cagatgctca tgcacgcaat gatactcaat
      121 ttataaaaaa gacgctcaga acatcattga aaatagtggg tatttcatca ttcttattgg
      181 ccttcatatt agtagtgttc ggtagtgaag tcgttaatat ttggacagaa ggaaagattc
      241 aggtacctcg aacattcata atagcttatg ctttatggtc tgttattgat gctttttcga
      301 atacatttgc aagcttttta aatggtttga acatagttaa acaacaaatg cttgctgttg
      361 taacattgat attgatcgca attccagcaa aatacatcat agttagccat tttgggttaa
      421 c
//
LOCUS       DAZFKY010000599          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFKY010000599 DAZFKY010000000
VERSION     DAZFKY010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.604"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(185..>421)
                     /locus_tag="ACHKCL_005315"
     CDS             complement(185..>421)
                     /locus_tag="ACHKCL_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766049.1"
                     /translation="AIRKEAVISLKRLESSNREFIRYRFAQCSFTLTWGARVGRLAFS
                     RLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT          134 a           68 c           78 g          141 t
ORIGIN      
        1 tcgaatgcta aatctacaaa gcaataatct acgtagaaca taaatctacg tatatcttcc
       61 ggtatagcag tggaaaaata acggtattgc gtgtaagtgg tttgatcatc gaattcgtct
      121 gattgtactg aatatgtaaa tgtggccagt ataaatagta atgttaatct taaatttata
      181 ataatcattt gctggatatc tcatctggat tttgaaagtc agaaatgtaa gcattgttta
      241 gttgcatggc tcttattttg tttaactcag tatagcatgg atatagccga gagaatgcca
      301 gtcgccctac tctggccccc caggttagag taaaagagca ttgagcaaat ctgtaacgta
      361 taaattccct attagaagac tctagtcttt ttaaagaaat aactgcttct tttcttattg
      421 c
//
LOCUS       DAZFKY010000600          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFKY010000600 DAZFKY010000000
VERSION     DAZFKY010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.605"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>418)
                     /locus_tag="ACHKCL_005316"
     CDS             complement(<1..>418)
                     /locus_tag="ACHKCL_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766050.1"
                     /translation="PVVDYYINNSNHNEQLSIPPYGTRSYKDTFQLIFYKNNIRYYKP
                     AIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEIDVSDWNKSQKDVFWKEYEK
                     QRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFL"
BASE COUNT          126 a           76 c           64 g          152 t
ORIGIN      
        1 atagaaactt atcattttca tccggcatcc caaaaaagaa aaccgtcaag tattttttag
       61 cattcctttt atctttaagc cccttataat acctatcagc ttccttcctt tgcttttcat
      121 attccttcca aaacacatcc ttctgacttt tattccagtc agaaacatca atttcaagcg
      181 taaatggttt ttgggttttc ttatcccttg cgaggaaagt aatacaatgt ttatctgaat
      241 cttcatgcag acgaatatta gactgggcta caccataata aatagcaggt ttgtaataac
      301 gtatgttgtt tttgtaaaaa ataagttgaa atgtatcttt ataagatcgc gtgccatatg
      361 gagggattga tagctgttca ttatgattgc tattatttat gtaatagtca acgactgg
//
LOCUS       DAZFKY010000601          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFKY010000601 DAZFKY010000000
VERSION     DAZFKY010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.606"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>412
                     /gene="wzx"
                     /locus_tag="ACHKCL_005317"
     CDS             <1..>412
                     /gene="wzx"
                     /locus_tag="ACHKCL_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO5766051.1"
                     /translation="VISSLISKILSLLSLILTVSLTLPYLGQERFGVWMTITSLGAAL
                     TFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAICYITSGMIDW
                     QLVIKGINENVYAELQHSIKVFVIIFGLGIYSNGV"
BASE COUNT          123 a           59 c           83 g          147 t
ORIGIN      
        1 ctgttatttc aagtttgatt agtaaaatac tctcactact ttctcttata ttaactgtaa
       61 gtttaacttt accttattta ggacaagaga gatttggtgt atggatgact attaccagtc
      121 ttggtgctgc tctgacattt ttggacttag gtataggaaa tgcattaaca aacaggatcg
      181 cacattcatt tgcgtgtggc aaaaatttaa agatgagtcg gcaaattagt ggtgggctca
      241 ctttgctggc tggattatcg tttgtcataa ctgcaatatg ctatattact tctggcatga
      301 ttgattggca actagtaata aaaggtataa acgagaatgt gtatgcagag ttacaacact
      361 caattaaagt ctttgtaatc atatttggac ttggaattta ttcaaatggt gt
//
LOCUS       DAZFKY010000602          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFKY010000602 DAZFKY010000000
VERSION     DAZFKY010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.607"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>412
                     /locus_tag="ACHKCL_005318"
     CDS             <1..>412
                     /locus_tag="ACHKCL_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5766052.1"
                     /translation="GETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          123 c          133 g           59 t
ORIGIN      
        1 gcggggagac agtacgctac cgctacgatg atgcgtacag tgagttaccg gcgacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacggtcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
      181 cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt ggccggttaa
      241 tctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggaatacgac ct
//
LOCUS       DAZFKY010000603          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFKY010000603 DAZFKY010000000
VERSION     DAZFKY010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.608"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>412
                     /locus_tag="ACHKCL_005319"
     CDS             <1..>412
                     /locus_tag="ACHKCL_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5766053.1"
                     /translation="GDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          117 c          131 g           67 t
ORIGIN      
        1 gtggggacgt catccggtat gcttatgata atccgcacag tgaattaccg gccacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacgggcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgtt atgaatacga ccgctttggt cagatgacgg
      181 cggtccaccg tgaggaaggt atcagccgtt accgccgcta tgacaaccgt ggccggttaa
      241 cctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcactccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aagcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtatgac ct
//
LOCUS       DAZFKY010000604          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFKY010000604 DAZFKY010000000
VERSION     DAZFKY010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.609"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..243
                     /locus_tag="ACHKCL_005320"
     CDS             <1..243
                     /locus_tag="ACHKCL_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5766054.1"
                     /translation="AEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            240..>406
                     /locus_tag="ACHKCL_005321"
     CDS             240..>406
                     /locus_tag="ACHKCL_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5766055.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          113 a           95 c          129 g           69 t
ORIGIN      
        1 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
       61 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      121 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
      181 gcagcaacca acaagaaaac actggcagaa tacgcccgtg ccttatccgg agaggatgaa
      241 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
      301 aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacgacc acttccgtgt
      361 ctgacctgaa aaaatacatt gctgagctgg aagtgcagac cggcat
//
LOCUS       DAZFKY010000605          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFKY010000605 DAZFKY010000000
VERSION     DAZFKY010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.610"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..398)
                     /gene="escQ"
                     /locus_tag="ACHKCL_005322"
     CDS             complement(<1..398)
                     /gene="escQ"
                     /locus_tag="ACHKCL_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO5766056.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSKGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDIYPVIENNNGEN"
BASE COUNT          121 a           95 c           71 g          117 t
ORIGIN      
        1 ctgttttctc cattattatt ttctattacc ggataaatat ctttatttag catcgtaaat
       61 tgaacatcat caactgacac atcaccttcc ggtaaggcag tctgcattac ccataagatc
      121 atgagtttta tcgtatcttc cggtatcagc cggctttcgc tatacgggaa taactcttgg
      181 caccactgaa tgaactgttc ttctgatatc cagaaacgcc cctcatagcc cgagtatgtc
      241 aacgtgaaac gaataccatg accgctgacc tcacgaaaat atgcatggca agcatgacct
      301 ttggacgtaa tatagaggcg accggttccg ataactggca acaacggcaa ataaaaatcg
      361 ttaattttca tattcaattg tgaactcaat ggcttcatca ggaa
//
LOCUS       DAZFKY010000606          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFKY010000606 DAZFKY010000000
VERSION     DAZFKY010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.611"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..>402
                     /locus_tag="ACHKCL_005323"
     CDS             63..>402
                     /locus_tag="ACHKCL_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5766057.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVIT"
BASE COUNT          143 a           59 c           81 g          119 t
ORIGIN      
        1 attaaacaaa aattatatct caccactaag gtttataaaa gcataagtta gcaggtgtca
       61 ccatgaaaaa agccatagca tatatgcgat tttcatcacc aggtcagatg tctggcgact
      121 cattaaaccg acagagaaga cttattgctg aatggttaaa ggtaaatagt gattattatc
      181 ttgataccat aacatatgaa gatttaggat taagtgcatt caaaggaaag catgcacaat
      241 caggagcttt ttcggaattt ttagatgcta tagagcatgg ttatatattg ccaggaacta
      301 cattgttagt tgaaagtctg gacagacttt caagagaaaa agtcggtgaa gcgattgaac
      361 gtctgaaatt gattttgaat cacggtattg atgttataac tc
//
LOCUS       DAZFKY010000607          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFKY010000607 DAZFKY010000000
VERSION     DAZFKY010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.612"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..78)
                     /locus_tag="ACHKCL_005324"
     CDS             complement(<1..78)
                     /locus_tag="ACHKCL_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766058.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKS"
     gene            96..287
                     /gene="yfgG"
                     /locus_tag="ACHKCL_005325"
     CDS             96..287
                     /gene="yfgG"
                     /locus_tag="ACHKCL_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5766059.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT           99 a          103 c           77 g          119 t
ORIGIN      
        1 cgatttttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa tgtaagtacc
       61 gtccacggcg tgggacatac ttcaaggaac cttttgtgag tcaggcaacc agtatgcgaa
      121 aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc tttatcttct
      181 tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag agcaaaaaag
      241 aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg ttaccttctc
      301 cactttcaca gaacataacg gcgcttcgct gtcggatgct tttgctcttt ggaattatca
      361 aagcggcaga tattctttca tcttaaatta tacgtctt
//
LOCUS       DAZFKY010000608          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFKY010000608 DAZFKY010000000
VERSION     DAZFKY010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.613"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..167
                     /locus_tag="ACHKCL_005326"
     CDS             <1..167
                     /locus_tag="ACHKCL_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766060.1"
                     /translation="EEQAEWLIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRP
                     SRTFQQRIWR"
     gene            169..>398
                     /locus_tag="ACHKCL_005327"
     CDS             169..>398
                     /locus_tag="ACHKCL_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766061.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
BASE COUNT           89 a          112 c          110 g           87 t
ORIGIN      
        1 ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa
       61 aagcccttag cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac
      121 ggccaccgcg tccgtcccgc acattccagc aacggatctg gaggtaatgt gcgggtatta
      181 cttcgacctg ttctggtacc ggaactcggt ctggttatcg ttaagccagg ccgtgaatca
      241 atgtcagcat tccataacgg cagaatactg gtggagccgg aaccaaaaag catgcgagct
      301 ctgccgtccg gggttgtacc tgccgttcac cagccgctgg cggaagataa atcactactg
      361 ccatttttca gcgatgagcg ggtgatccgt gctgcggg
//
LOCUS       DAZFKY010000609          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFKY010000609 DAZFKY010000000
VERSION     DAZFKY010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.614"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>397
                     /locus_tag="ACHKCL_005328"
     CDS             <1..>397
                     /locus_tag="ACHKCL_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5766062.1"
                     /translation="TTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRI
                     MGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERT
                     KGKQVAVTEAMSLFADVMNARLEQLDKAAT"
BASE COUNT          102 a           88 c          134 g           73 t
ORIGIN      
        1 caccacaacg gcagaattgt ggaaaatggc cggggtgatc agcgatgaag ccggtggtgg
       61 tatcagacag gcggcagaaa atctggcccg tctggcagaa agcgggaaat ataccgccgg
      121 gcagctacgg atcatggggg aaacctctca gagatggctg cagacggtgg gggacgatgc
      181 cgggaaggtg gaaaaagcct ttgaagggat tgcagcagat ccggtgaagg cgctggcctc
      241 cctgaatcag cagtataact tcctgagcgt ttcccagtta cgccatattg atgagcttga
      301 gcgcacgaaa ggtaaacagg ttgcggtgac ggaggcgatg tccctgtttg cggatgtcat
      361 gaatgcacgt ctggagcaac ttgataaagc ggccacg
//
LOCUS       DAZFKY010000610          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFKY010000610 DAZFKY010000000
VERSION     DAZFKY010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.615"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>392
                     /locus_tag="ACHKCL_005329"
     CDS             <1..>392
                     /locus_tag="ACHKCL_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5766063.1"
                     /translation="ADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWG
                     TLKNESLSHYRFNNRDEAISVIREYIEI"
BASE COUNT          108 a           91 c          107 g           86 t
ORIGIN      
        1 tggcggacct gacgtatgtt gccacacagg agggatggtt gtacctcgct ggcatcaaag
       61 atgtttatac gtgcgaaatt gtcggctacg ccatgggaga gcgcatgaca aaagagctga
      121 caggtaaagc cctgtttatg gcgctcagga gccagcgccc acctgccggg ctaatccacc
      181 actctgatcg aggttcacag tactgcgcat acgattaccg ggtcatacag gagcagtttg
      241 gtctgaaaac atcaatgtcg cgtaaaggta actgttacga caacgctccg atggaaagct
      301 tctggggaac gctgaaaaat gagagcctga gccactatcg ttttaataac cgggatgaag
      361 ccatctcagt aatacgggaa tacattgaga tt
//
LOCUS       DAZFKY010000611          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFKY010000611 DAZFKY010000000
VERSION     DAZFKY010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.616"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>390)
                     /locus_tag="ACHKCL_005330"
     CDS             complement(<1..>390)
                     /locus_tag="ACHKCL_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5766064.1"
                     /translation="GDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADW
                     TATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVA"
BASE COUNT           71 a          135 c           85 g           99 t
ORIGIN      
        1 tccgccacgg aaggtttgcc cacgttagtg actttcaccg tgcgggtgat cacttctttc
       61 gccgtcacgg ccttaccgat actgctgacc cagccacgga acacatccac cgtgccattt
      121 gggaaacgga ttttataggc ccgcacatcc ccgctttcaa accagcctat aagccctttc
      181 tgaccttctt ctcccggttt ccaggccagc gtaaaactgg tatctcctgc agacttctgc
      241 ccctgcccgg tcgcggtcca gtccgcgtct tcatcatcca ggtagttatc atcgtagggt
      301 tctgccgtca tctcgcccgg cgtcagatcc ttcaccttag ccagtcgctg ccagtcatcg
      361 tctgacaacg ggtttgcata agcatcaccc
//
LOCUS       DAZFKY010000612          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFKY010000612 DAZFKY010000000
VERSION     DAZFKY010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.617"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..>384
                     /gene="tccP2"
                     /locus_tag="ACHKCL_005331"
     CDS             31..>384
                     /gene="tccP2"
                     /locus_tag="ACHKCL_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO5766065.1"
                     /translation="MSSAIRSSTFTVSQHKSTPNTVKTSSPFSPSNSPASATTIFKVK
                     NSYTESGLQRPTSYTQSSIEKNALHRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAP
                     NWPAPPPPVQNEQSRP"
BASE COUNT          113 a          113 c           64 g           94 t
ORIGIN      
        1 taattctttt tttccggcct tcctcgcagt atatcaagtg ctatacgcag cagtaccttc
       61 actgtctcgc agcacaaaag cacacctaac acggtaaaaa ccagctcacc tttttctccc
      121 agcaacagtc ctgccagtgc aacaactatt tttaaggtaa aaaattctta tacagaatca
      181 ggacttcaac gtcccacatc ttatactcag tcaagtatag aaaaaaacgc tttacatcgg
      241 cctctacctg atgtagctca gcgtctggtg cagcatcttg cagagcatgg cattcaacca
      301 gcccggagta tggctgaaca tattcctccg gcacctaact ggcctgcgcc accaccgcca
      361 gtacaaaatg aacaatcaag acct
//
LOCUS       DAZFKY010000613          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFKY010000613 DAZFKY010000000
VERSION     DAZFKY010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.618"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>370
                     /locus_tag="ACHKCL_005332"
     CDS             <1..>370
                     /locus_tag="ACHKCL_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5766066.1"
                     /translation="AAGYSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQ
                     KKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYMEDGLPDPVKAMGRLLVEN
                     INTDPRLALDAAYKLAQFTH"
BASE COUNT           89 a           90 c          117 g           74 t
ORIGIN      
        1 ttgccgctgg ttattcggcg aagaccgcca gggttaaagg ctcgcagctg gcaaaagatc
       61 cggaggtgct tgcgtttata gcccgtaaac aatgcgagac ggtggaggtg gatgaggttc
      121 ctgtttaccg gcagaaaaaa tcagagcagg aggataaacc ccgtcgccgt gaggcggctg
      181 caataccaca gccggacgaa aacaatccgg agatgccacc gtccgcggtg atgtctcctg
      241 gtattgaata tatggaggat ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg
      301 tggagaacat taataccgac cccaggctgg cgctggatgc ggcttataag ctggcgcagt
      361 tcacgcacca
//
LOCUS       DAZFKY010000614          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFKY010000614 DAZFKY010000000
VERSION     DAZFKY010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.619"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>368)
                     /locus_tag="ACHKCL_005333"
     CDS             complement(<1..>368)
                     /locus_tag="ACHKCL_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5766067.1"
                     /translation="HSKIVQERYASLSFGETDKISRSTRLDPNGFCPTLRAGTARDKG
                     SFQAVRPIHPYHPRVITPREAARLQGFPDWFRFHVTKWHSFRQIGNSVSPIVAEYILK
                     GLYNLLNKRVQPEYLNHNSL"
BASE COUNT          103 a           73 c           70 g          122 t
ORIGIN      
        1 ccaaagaatt atggtttaaa tattcgggct gtactctttt atttaataaa ttgtacagcc
       61 cctttaatat atattcagca actattggtg acacgctatt tcctatttgt ctgaaactat
      121 gccatttagt tacatgaaag cgaaaccaat cagggaagcc ttgtaatcta gcagcttctc
      181 ttggtgtaat cactcttgga tgataagggt ggatcggcct gacggcttga aagcttcctt
      241 tatctctagc ggttcccgcc cttaaagtcg ggcaaaaacc atttggatct aatcttgtag
      301 atcttgaaat tttatcagtt tccccaaaag ataaggaagc ataacgctct tggacgattt
      361 tgctatgt
//
LOCUS       DAZFKY010000615          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFKY010000615 DAZFKY010000000
VERSION     DAZFKY010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.620"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..355)
                     /locus_tag="ACHKCL_005334"
     CDS             complement(<1..355)
                     /locus_tag="ACHKCL_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HFO5766068.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLF"
BASE COUNT          154 a           78 c           47 g           89 t
ORIGIN      
        1 gaaacaaata atagattgca atgtcatttt ggacaacact gcttgtcaaa cgcccctcaa
       61 taatttgctg aatgataatg acggttttaa caaagtaaat caaacaactc agaaaagcca
      121 aactaagtat acagtttgaa aattgcctac ggtcataaat gaaatacaac tgcataaaaa
      181 taaatgcaac agtatcgata aaaaataaag caatcattac catcagatag gttgatcgtg
      241 gaacaacttc ctcaacgcct ggctgattaa accaactatt caccaaaacc aaagcaagcc
      301 caaacaataa gctcactaat acagtactac taattctcaa atagtctttt tccataaagc
      361 tacaccaa
//
LOCUS       DAZFKY010000616          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFKY010000616 DAZFKY010000000
VERSION     DAZFKY010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.621"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>368
                     /locus_tag="ACHKCL_005335"
     CDS             <1..>368
                     /locus_tag="ACHKCL_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5766069.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMET"
BASE COUNT           84 a           82 c          115 g           87 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaac
//
LOCUS       DAZFKY010000617          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFKY010000617 DAZFKY010000000
VERSION     DAZFKY010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.622"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>367)
                     /locus_tag="ACHKCL_005336"
     CDS             complement(<1..>367)
                     /locus_tag="ACHKCL_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766070.1"
                     /translation="NNKLQPTEIYKESQQTAKLEFFCDEFLKISQSRYGLSTSTDSIA
                     NLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFTR
                     GENNEGILSVESKEGFISQR"
BASE COUNT          101 a           61 c           67 g          138 t
ORIGIN      
        1 tctttgtgaa ataaaccctt ctttgctttc gacactcaaa ataccttcgt tgttttcacc
       61 tcgggtaaag gtaagggtat agggttcata atccgctgca aattcatttt tatacgttat
      121 gacatgttcg acaaaatgtc ttattggtac gaaaccataa ctgtgaacgt caattttttg
      181 ggtcttgata ctatcaattg catctgaggc tttcgtgaaa aaagccaata aattagctat
      241 gctgtctgtt gaggttgata acccgtatct actttgagat atttttagaa actcatcgca
      301 aaaaaactca agtttggcgg tttgttgtga ttctttgtaa atttctgttg gttgcaattt
      361 attattt
//
LOCUS       DAZFKY010000618          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFKY010000618 DAZFKY010000000
VERSION     DAZFKY010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.623"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(25..291)
                     /locus_tag="ACHKCL_005337"
     CDS             complement(25..291)
                     /locus_tag="ACHKCL_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO5766071.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
BASE COUNT          103 a           65 c           62 g          134 t
ORIGIN      
        1 cgcaaaaaac gtgtagattt ttatttaatg agttaaacga tcttgcgaaa tgacaagcaa
       61 ttgatacata ctcatctctt tataagctgg aactgtagta acctgtgcaa attcgcgata
      121 tagcttttcc tccagttctg ctgatgatat atattccggg tgttcgctaa agaactgtct
      181 tgcatctact atcgcttggg attcgccact tcttttattt tgattctcct ttaatgaatt
      241 ttctctggtg catgcagaga gcaacgatat cagcgcgatg gttattatga ttttactatt
      301 atatcattac taatgacata tcaactttct cttacgcaag attggagttt aaaataatgt
      361 ttat
//
LOCUS       DAZFKY010000619          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFKY010000619 DAZFKY010000000
VERSION     DAZFKY010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.624"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..192
                     /locus_tag="ACHKCL_005338"
     CDS             <1..192
                     /locus_tag="ACHKCL_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766072.1"
                     /translation="EASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGSASGG"
     gene            237..>357
                     /locus_tag="ACHKCL_005339"
     CDS             237..>357
                     /locus_tag="ACHKCL_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766073.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITA"
BASE COUNT           68 a           98 c          132 g           59 t
ORIGIN      
        1 gaagcatcac tggaaatgca gtctgagccg accggcgaca gtaccacgcc gtccccggtg
       61 gagctggttt ccatgttcca gacaggcagc gtggccatcc gtgcggagcg ctggatcaac
      121 tggcgtcgtc gccgtaccgc ggcggtggcg gtgatcaccg gagtgaacta cggcagtgcg
      181 tccggcggct gagtctgatg aggagggcgg gaggcgcgag cttcccgcag taacagatgg
      241 caaaaatcca gtatctgcag ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg
      301 cacagccgtg tgcggaggtg ctggttcgcc tggggaaggc ggagtacatc acagcgc
//
LOCUS       DAZFKY010000620          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFKY010000620 DAZFKY010000000
VERSION     DAZFKY010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.625"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            14..>353
                     /locus_tag="ACHKCL_005340"
     CDS             14..>353
                     /locus_tag="ACHKCL_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5766074.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDN"
BASE COUNT           92 a           82 c           91 g           88 t
ORIGIN      
        1 acggaggtaa tacatgtttc tgaaaacaga acaatttgaa tataacggtg tgtccgtcac
       61 gctttccgaa ttgtctgcgc tgcagcggtt tgattatata aagtttgttt cagacgcaga
      121 acaacaggag acaacgaagc atgatgtcgt gcacattaac cagcgatatc tggaaacggc
      181 atccctgctt gtggcgatgt cgctatggca ttcccattcc ctcaaaggca ctctggcctc
      241 tccggagaca gagatgcagc agatccgccg tgaagtgatg ctgggatggc ctgctgatgc
      301 actgaatcag gcaacgaacc gggtgcttta tctttcaggt atgctggata acc
//
LOCUS       DAZFKY010000621          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFKY010000621 DAZFKY010000000
VERSION     DAZFKY010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.626"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..145
                     /locus_tag="ACHKCL_005341"
     CDS             <1..145
                     /locus_tag="ACHKCL_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5766075.1"
                     /translation="RAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKAYYASIRNDD
                     LAA"
BASE COUNT          103 a           92 c           80 g           78 t
ORIGIN      
        1 ccgtgcagaa gtggaactgg ccacactcac gtgggtggac tggtataaca atcgacgatt
       61 gctgggaagg ctgggtcata tccctccggc agaagcagaa aaagcttatt atgcttccat
      121 cagaaacgat gatctggcag cctgagttca cagataaaac actctccagg aaaaccgggg
      181 cggttcacca gcctcctgac gatacctccc accatgtttt gcgatggcac gctaccgccc
      241 ttccggtatt tacccgcatc aaccggtacc agaatgctga gtaacaaacc agctagaatt
      301 gacttaattt tgatcattat agaataatga tctgaactac agcaacatga cag
//
LOCUS       DAZFKY010000622          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFKY010000622 DAZFKY010000000
VERSION     DAZFKY010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.627"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>348
                     /locus_tag="ACHKCL_005342"
     CDS             <1..>348
                     /locus_tag="ACHKCL_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5766076.1"
                     /translation="KGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLAL
                     EAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETL
                     EYKRQLAELGDKVE"
BASE COUNT          104 a           79 c          116 g           49 t
ORIGIN      
        1 aaaggtccgg ggcagcagga agccgtcagc cgtctggctg aagagctgac aggtaaaaag
       61 catacatcac cgaaaacgcg ctctgccggg gagagggaag aggagcaggc aagagaggct
      121 ctgcttgccc ttgaagctga gctcaggacg ctggaaaaac acagcggtgc gaatgagaaa
      181 atcagccggc agcgccgtga tttatggaag gcggaaagtc agtatgcggt cctgaaagag
      241 gctgccacga aacgacagtt atctgagcag gaaaaatccc tgctggcgca taaagacgag
      301 acgctggagt acaaacgcca gctggctgag ctgggcgaca aggttgaa
//
LOCUS       DAZFKY010000623          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFKY010000623 DAZFKY010000000
VERSION     DAZFKY010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.628"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>346
                     /locus_tag="ACHKCL_005343"
     CDS             <1..>346
                     /locus_tag="ACHKCL_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5766077.1"
                     /translation="LSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNL
                     RCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLN
                     NDVYKELKKFMHD"
BASE COUNT          128 a           64 c           55 g           99 t
ORIGIN      
        1 ttatccaata tatcatgttt tgaaagcgac ttatcaaaca ccatcttttc aaatggagaa
       61 ataaacaacc tcttcattaa aaaaagcaac atatttggaa catcgttcac taataccatg
      121 ataaaaaatt tgcgctgtga agatattatg cctggccgat ggaccactca acttgtcaat
      181 aagcacttag gttacagata cacaggagta ttcaaaaccc tggcaagtat tgatgacaaa
      241 ccgtcccgtt tcgaaatttt gataccattg gtacaaactt tagttcgcga caatgtaaag
      301 ctaaataatg atgtgtataa agaactgaaa aagtttatgc acgatt
//
LOCUS       DAZFKY010000624          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFKY010000624 DAZFKY010000000
VERSION     DAZFKY010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.629"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            246..>345
                     /locus_tag="ACHKCL_005344"
     CDS             246..>345
                     /locus_tag="ACHKCL_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766078.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKR"
BASE COUNT           90 a           69 c           92 g           94 t
ORIGIN      
        1 gataattatt atcattttgc gggtcctttc cggcgatccg acaggttacg gggcggcgac
       61 ctcgcgggtt ttcgctattt atgaaaattt tccgggatcc atgtccggtt tctcttcaag
      121 ttaactatat gaaaaatata aaaacaggtc ttctgtgaac cggacatgaa caaaaaacag
      181 acatgtaaac cggacatgac cggttttgtt gtgattgtga ggtgagagtt tttgcgaggt
      241 gaggagtggc tacgcagact gaagttgcca ggcatttaag tctgaccgat cgccagcttc
      301 gcagattgca gaaattgccg ggtgccccga tatcgaataa gcgag
//
LOCUS       DAZFKY010000625          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFKY010000625 DAZFKY010000000
VERSION     DAZFKY010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.630"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          101 a           64 c           68 g          109 t
ORIGIN      
        1 tatatctttt acgtcggggt agattcttta tggtcattac gggcaggcga catttcatta
       61 tattaccatt tgcattcacg catgtaaccc ttaagagtta tataaaaagg aaagagttct
      121 tgctcatggt attactgaga tcggattaac catgaggtta gctatataga gagctttggc
      181 tttgcgacaa ttggcttaag cagccacatt atgcctggct tatcgttgaa agggagaaag
      241 aagagcaaaa cggtttggca agctttcatt cccatctaat agcaatcacc gctatacatc
      301 atcccttaac gtattctggt cttcataaaa atgttattct aa
//
LOCUS       DAZFKY010000626          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFKY010000626 DAZFKY010000000
VERSION     DAZFKY010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.631"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>339
                     /locus_tag="ACHKCL_005345"
     CDS             <1..>339
                     /locus_tag="ACHKCL_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5766079.1"
                     /translation="VIDKGSKVQLEVYNLLSEEKIFEHKIMNNWTRSIKNILTTYLFM
                     SSGAVTARNVQTFSPTINNESRIRLPRALPVGHPYPEEHKASGFSPFMMGGLSGDILP
                     EILTGNGPSI"
BASE COUNT          115 a           52 c           74 g           98 t
ORIGIN      
        1 agtaatcgat aaaggaagca aagttcaatt ggaggtatat aatttattat ccgaagaaaa
       61 gatatttgaa cataaaatta tgaataattg gacaaggagc ataaaaaata tattgacgac
      121 atatttgttt atgtcatcag gagcggtgac agccagaaat gttcaaacct tttctccaac
      181 aataaataat gagtcaagga ttcgattgcc gagagcattg ccagtaggcc atccatatcc
      241 tgaggaacat aaggcttctg gtttctcccc ttttatgatg ggggggctga gtggtgatat
      301 tcttccggaa attttaacgg ggaatggacc atctatatt
//
LOCUS       DAZFKY010000627          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFKY010000627 DAZFKY010000000
VERSION     DAZFKY010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.632"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          112 a           63 c           55 g          109 t
ORIGIN      
        1 cggagatcat ttccatgaat gatttcatca gtcgaactat ccttcgcaat cgtcatatct
       61 taaccctatg aaaatctatg tccttctaag gattctagct ttttcttatt gacgatccaa
      121 tcaaaaaatt gtactccaac ttaccaaaat acacgatcaa gcagaagtta ttaacttaag
      181 gggtagaatt agtatttttc aaagaaatta aatctttgac agaatgatgc tgaataacat
      241 cgctaacctt agtaaggtca gggagttgtt atacacaact cccctctatt atcgtttgtt
      301 aaaggttctg ggtatgtatg catggtaaca gtaaaagca
//
LOCUS       DAZFKY010000628          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFKY010000628 DAZFKY010000000
VERSION     DAZFKY010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.633"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          108 a           70 c           65 g           94 t
ORIGIN      
        1 gtcatcatag atgttatgca gtacgcacaa aaaaacagac tggatttgct tgctgatcta
       61 taatctttat ctaaattact attggcgctt tggataataa cataagcata tattacctcc
      121 tagcaataat agatataacc tcatatcaag gtgtcactgg taaataatcg gataaaagac
      181 agtaccggtc ggcacataga gcttcttcta tttcaccaga agtgcacagc agggaactac
      241 caatcacacg gttagtgtca tgggtggagg atgcgtcccg ttctttccct ataacgggga
      301 tacgcagttt ttatcagtac atgcagaaaa accccta
//
LOCUS       DAZFKY010000629          334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFKY010000629 DAZFKY010000000
VERSION     DAZFKY010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.634"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..315)
                     /locus_tag="ACHKCL_005346"
     CDS             complement(43..315)
                     /locus_tag="ACHKCL_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5766080.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT           65 a           94 c           87 g           88 t
ORIGIN      
        1 gaaaaaggcc agcagctgtt tactgatggc cctgactccc cgttacagca tcatgaccga
       61 ttcgggttga ggttcagtcg catcggcgac cggtgattca ggctgaactt caccgctctc
      121 tgcggtggta tctcccgctt cagtcggtgg ctctgcctgt acaccaagca gttcatccag
      181 aatggcatcc acttctgcat caagacgcgc ttccaggtta tggcgaagtt tctgtttcag
      241 tgcgctccgg acttcttcag agcgcaggac gtccttcact gcctctgcag tgaccaggga
      301 tgtaatttct gacatgggat tttctcgtcg aaag
//
LOCUS       DAZFKY010000630          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFKY010000630 DAZFKY010000000
VERSION     DAZFKY010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.635"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>333
                     /locus_tag="ACHKCL_005347"
     CDS             <1..>333
                     /locus_tag="ACHKCL_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5766081.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZFKY010000631          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFKY010000631 DAZFKY010000000
VERSION     DAZFKY010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.636"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>327)
                     /locus_tag="ACHKCL_005348"
     CDS             complement(<1..>327)
                     /locus_tag="ACHKCL_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766082.1"
                     /translation="GNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQTSIEMVT
                     LLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTI
                     NEPLKNQ"
BASE COUNT           94 a           53 c           62 g          118 t
ORIGIN      
        1 ctggtttttt aacggttcat taatggtaag ttcacaagta ttgtccgatc gttgaataat
       61 ttgggcgtta taacctaaaa aatccagacg aagagacgag tcattataaa gaattacatt
      121 ttgtaagtac gctaattctt tttgaaaatc accagtgata atgcattttc cactaggttg
      181 taacgtgtta agtaaagtga ccatttcaat agaggtctgt tttagttttt caatacctgg
      241 tgctagtgct tcgaggttat ttcctgttgg gctttcaggt aggtcattca tttttcgacg
      301 gactaaatca ctactaatat cattacc
//
LOCUS       DAZFKY010000632          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFKY010000632 DAZFKY010000000
VERSION     DAZFKY010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.637"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..218
                     /locus_tag="ACHKCL_005349"
     CDS             <1..218
                     /locus_tag="ACHKCL_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766083.1"
                     /translation="VSIKQPQNIPSKQVKNKTAPEQLITTENAFSEPPKLLRILFIAH
                     VNALYNSSSPRYLTSLLFNYNSNKSRH"
BASE COUNT          117 a           81 c           39 g           90 t
ORIGIN      
        1 tggttagcat caaacaacct cagaatattc caagcaaaca ggttaaaaat aaaactgcac
       61 ccgaacaatt gataacgaca gaaaacgcct tttccgagcc accaaaatta cttcgcatcc
      121 tatttattgc tcacgttaac gccttgtata actcgagctc tccacggtat ttaacctctc
      181 ttctgtttaa ctataattcc aataaatctc gtcactgagc tcaatcactt cgaaagtcaa
      241 gcacaatatg aatttcacat aagctatcaa tgaaaatcgg aggtcatacc attaatcaac
      301 aataaaccaa gtctttctca tataatc
//
LOCUS       DAZFKY010000633          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFKY010000633 DAZFKY010000000
VERSION     DAZFKY010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.638"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>326)
                     /locus_tag="ACHKCL_005350"
     CDS             complement(<1..>326)
                     /locus_tag="ACHKCL_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5766084.1"
                     /translation="DVAVTESYKPIWNWKISQYNVSDIKFETIIKPQFADRIYFSNCS
                     PVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLR
                     ENFNEQ"
BASE COUNT           97 a           67 c           62 g          100 t
ORIGIN      
        1 cttgctcatt aaagttctcc cttagcaata cattgagaac ttcatcttct atattaagca
       61 actggctaag gaaattttct ccttcagcca tgattcccaa atgtcccgtt gcaaaaagcg
      121 cgtacaaaac tgcaccaact gagccatcgg tatcaccaaa ataggttggt ctggtacttg
      181 tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg ggttttataa
      241 ttgtttcgaa tttaatatca gatacattgt attgcgatat tttccagttc cagatgggtt
      301 tgtacgactc agtaactgca acatca
//
LOCUS       DAZFKY010000634          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFKY010000634 DAZFKY010000000
VERSION     DAZFKY010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.639"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>326
                     /locus_tag="ACHKCL_005351"
     CDS             <1..>326
                     /locus_tag="ACHKCL_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO5766085.1"
                     /translation="KERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHV
                     DMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQA
                     YKTDPQ"
BASE COUNT          105 a           46 c           75 g          100 t
ORIGIN      
        1 tcaaagagag atttattgag gcacttaatt ccaataaatc agattataaa atgattgctg
       61 atgatcagca aaggaaactt gcctgtgtct ggaatccctt tcttgatggt tgggaactga
      121 acgctcagca tgtagatatg attatgggga gccatgtatt gaaagatatg ccactaagaa
      181 aacaggctga aatattattt tgtttagggg gggttttctg taaatactca tcgagtgata
      241 tgtttggtac agagtatgat tctcctgaga ttctacggag atatgcaaat ggattgattg
      301 aacaagctta taaaacagat cctcag
//
LOCUS       DAZFKY010000635          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFKY010000635 DAZFKY010000000
VERSION     DAZFKY010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.640"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..>321
                     /locus_tag="ACHKCL_005352"
     CDS             63..>321
                     /locus_tag="ACHKCL_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO5766086.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMM"
BASE COUNT          116 a           64 c           60 g           81 t
ORIGIN      
        1 tcaacattca tttgtccagc actccatact ttcagccgtt ggattggtat ataataaaag
       61 ccatgctctg gactgaaaac gatgcggaaa atacttctca gtggaatggt tatccattac
      121 aaattggtcg ttttcggaaa gataaagcga tgccagcttt aatctctgga gaaaaatcta
      181 cagcccttgt taccccacca caatggagaa acaaagcatt caatgggtta aaagatccag
      241 aaagaaatta ctgggcaaaa gaacaaataa ctggctctcc agaagagaat atcaaagcag
      301 caattactta cttaatgatg a
//
LOCUS       DAZFKY010000636          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFKY010000636 DAZFKY010000000
VERSION     DAZFKY010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.641"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="ACHKCL_005353"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="ACHKCL_005353"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5766087.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          106 a           56 c           81 g           77 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
      241 gaggcgataa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
      301 ataacaactg ctgaaattat
//
LOCUS       DAZFKY010000637          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFKY010000637 DAZFKY010000000
VERSION     DAZFKY010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.642"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          107 a           56 c           66 g           88 t
ORIGIN      
        1 catgtaaaac gttcttgttt tttctccaca ccaccgtgga caatttttta ctgcaaaaag
       61 acgaggtttg tcacggcttg tgcgcaagac atatcgcagc aatcagcgac gggcaagaag
      121 aatgactgtc tggtgttttt tgctagcgga aaacggagat ttaaaagaaa acaaaatatt
      181 ttttgcgtag ataacagcgt atttacgtgg gttttaatac tttggtatga acaaaaaaag
      241 aaaaatacaa cgcgcgggtg agttattaaa aatatttccg cagacatact ttccatcgta
      301 acgcagcgtt aacaaaa
//
LOCUS       DAZFKY010000638          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFKY010000638 DAZFKY010000000
VERSION     DAZFKY010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.643"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..172)
                     /gene="cesF"
                     /locus_tag="ACHKCL_005354"
     CDS             complement(<1..172)
                     /gene="cesF"
                     /locus_tag="ACHKCL_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO5766088.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESI"
BASE COUNT          106 a           55 c           58 g           98 t
ORIGIN      
        1 gtattgattc gctattcaat aaataacaat aaaaaaacag aatttcagat tgagatgcac
       61 actccagtat ccattcatgg ccatcgttat cgtggaaaga gagcacgtca gtttgctctt
      121 caagagctat cccaaacgta tgtgcaaggt ctgtgcgaaa tttctcattc atattgtagt
      181 cctgccattc ttttcatgtt gatatactca aacgtaatta atccgagagt tctgatacaa
      241 ataataaggc gtagatataa gattacggga atagtgttgc atattcatga tagcatatga
      301 taaatgtagt gcaacta
//
LOCUS       DAZFKY010000639          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFKY010000639 DAZFKY010000000
VERSION     DAZFKY010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.644"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           96 a           66 c           60 g           92 t
ORIGIN      
        1 aaaattccag gtcttctttt tctgattacc gaagacaaag tggcaattac gtatagtttc
       61 ccgataaaga cgcgacagcg acatcccgca taaggcattt tttctttatc tttgtacggt
      121 acttcatgga acagagtttt tgaccttgcg aatcgtgatg tctgttgggg gcaatttact
      181 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
      241 ttttacaagg caaccgggta aaaataattt ttatttttta actgccctga gactcataaa
      301 gatgcctcaa aact
//
LOCUS       DAZFKY010000640          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFKY010000640 DAZFKY010000000
VERSION     DAZFKY010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.645"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..207
                     /locus_tag="ACHKCL_005355"
     CDS             <1..207
                     /locus_tag="ACHKCL_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5766089.1"
                     /translation="MRIIETNSYGWFPGTDGALITGLTFLDPKDATQVQGMFQHLQVR
                     FGDGPWQDVKGLDEVGSDTGRTGE"
     gene            170..>311
                     /locus_tag="ACHKCL_005356"
     CDS             170..>311
                     /locus_tag="ACHKCL_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5766090.1"
                     /translation="MKWAVIQAELENDMNILRKLMQSLCGCGKHDDCENGRSLTAQLR
                     LGP"
BASE COUNT           85 a           59 c           96 g           71 t
ORIGIN      
        1 atgagaatta ttgagacaaa cagctatggc tggttcccgg gtacagatgg tgcgctcatc
       61 accggactga cctttcttga ccccaaagat gccacacagg ttcaggggat gtttcagcat
      121 ttgcaggtca gatttggtga cgggccatgg caggatgtta agggactgga tgaagtgggc
      181 agtgatacag gcagaactgg agaatgacat gaatattttg agaaagctta tgcagagtct
      241 gtgcggttgc ggaaagcatg atgactgtga aaacggacgg tcacttacag cacaattgcg
      301 actgggaccg g
//
LOCUS       DAZFKY010000641          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFKY010000641 DAZFKY010000000
VERSION     DAZFKY010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.646"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..306
                     /locus_tag="ACHKCL_005357"
     CDS             <1..306
                     /locus_tag="ACHKCL_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766091.1"
                     /translation="LELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWTSGTHYTG
                     GSKRLDAGIIDITSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFGGIDINDFRL"
BASE COUNT          110 a           50 c           61 g           87 t
ORIGIN      
        1 ctagaattaa caggtagtaa atttagagtt gagattcaac cagccataaa aaatggagag
       61 cgagttcctg cgacaacgaa tggaccattt aaagtaaatg gtaagtggac atccggcact
      121 cattatacag gtggttccaa acgtctagat gccggtatta ttgatatcac atctcctaca
      181 aaccaatatg gattacatcc agttattgaa ggatttgata taacacttaa taaaacaaaa
      241 ccatcagcag tggatatata ttcagatgtg tttggtggga ttgatattaa cgactttcgg
      301 ttataaaa
//
LOCUS       DAZFKY010000642          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFKY010000642 DAZFKY010000000
VERSION     DAZFKY010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.647"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>308)
                     /gene="espR4"
                     /locus_tag="ACHKCL_005358"
     CDS             complement(<1..>308)
                     /gene="espR4"
                     /locus_tag="ACHKCL_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFO5766092.1"
                     /translation="IRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSN
                     DLKENVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPE
                     S"
BASE COUNT           95 a           68 c           51 g           94 t
ORIGIN      
        1 gattcgggaa cctgtattga atcaagttca ttataaacaa ggctaagcat ttcaatccaa
       61 tctggaagag gcggcacaga acccaaaaat aggtatgata agtcaagttc attactgtga
      121 agcttaacac atttaaacat tctcaccaac gctctatcta cattttcttt gaggtcattc
      181 gatccgcctt ctttgactga actagcccag tcactgaagc ctttgcaaag tctggcatct
      241 gtaattttcg ttaacttgtc cagcaactct gcaatattag tcgatataaa ctctaattta
      301 tgccgaat
//
LOCUS       DAZFKY010000643          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFKY010000643 DAZFKY010000000
VERSION     DAZFKY010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.648"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(44..289)
                     /locus_tag="ACHKCL_005359"
     CDS             complement(44..289)
                     /locus_tag="ACHKCL_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5766093.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT           63 a           96 c           76 g           73 t
ORIGIN      
        1 taaaaaagac caccggcaca caccgatggt ccctgacgca tgcttacatc atcatgtcgc
       61 tgtccggtgt ggggtcaccg ctatctgaag cactcccctc acccgcgatg ccttccggct
      121 ccggagctgc cggtgcgccc agcagctcat ccagaatggc atcaacctct gcatcaagac
      181 gcgcttccag gttatggcga agtttctgtt tcagtgcgct ccggacttct tcagagcgca
      241 ggacttcctt cactgcttca gcagtgacca gggatgtaat ttctgacatg ggattttctc
      301 gtcgaaag
//
LOCUS       DAZFKY010000644          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFKY010000644 DAZFKY010000000
VERSION     DAZFKY010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.649"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..286
                     /locus_tag="ACHKCL_005360"
     CDS             <1..286
                     /locus_tag="ACHKCL_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766094.1"
                     /translation="QWEQKFARHVQHVRAQVKPVSKGVSHAAPGGTASRAVQEIRAAR
                     EQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAVEALDCSDWRDD"
BASE COUNT           82 a           72 c          102 g           50 t
ORIGIN      
        1 tcagtgggag cagaaatttg cccgtcacgt gcagcacgtc agggcacagg taaaaccagt
       61 cagcaagggg gtaagccatg cagcaccagg tggcaccgca tcacgggcag ttcaggaaat
      121 tcgggcagca cgtgaacagt gggaacgtga aaacggattt atcagcaacg gaaacggcct
      181 ggaagctgtg ggaacttatg ggggaggtgt attcgaaccg ctggacccag aagaacgggg
      241 ctgcgccgtc gaagctctgg attgctcaga ttggcgcgat gactgaacag caaatccggc
      301 aggtct
//
LOCUS       DAZFKY010000645          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFKY010000645 DAZFKY010000000
VERSION     DAZFKY010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.650"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..65
                     /locus_tag="ACHKCL_005361"
     CDS             <1..65
                     /locus_tag="ACHKCL_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5766095.1"
                     /translation="MAMQKQLEGAQEYIRTQCIP"
BASE COUNT           98 a           42 c           77 g           87 t
ORIGIN      
        1 tgatggcaat gcagaagcaa ctggaaggag cacaggaata tatccgtacc cagtgtatac
       61 cgtgatgttt tgttatgagg gtgttactgg taacgttaag gtaatttaac aaagagtcag
      121 ttccggactt tatagtgtgc tcagttcatg gccaaaaacg atttctgtga taaatatttt
      181 gaatattatt tacaggtaaa tggagtgggg cacatggata gaaatattac aatagagaat
      241 gaagtatatg cccgtattgt atgggcagag aaggcaaaaa cacggtaatt ccgtgtgttg
      301 ccat
//
LOCUS       DAZFKY010000646          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFKY010000646 DAZFKY010000000
VERSION     DAZFKY010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.651"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..>302
                     /locus_tag="ACHKCL_005362"
     CDS             41..>302
                     /locus_tag="ACHKCL_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766096.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIF"
BASE COUNT          105 a           67 c           45 g           85 t
ORIGIN      
        1 ctggggatta atttttatta ctcacggaaa gatttaaata atgataccat ctcagatctc
       61 tttcaatacc cttccagtca atgcaacata cgcttcagag acaacagttg acacacaaaa
      121 attctctgac atcctctact cagcggggtg ttccctaaag gatatggctt atctgagcag
      181 atgtctggca tcaatacacc cgactctcgc caaaaacatt tatgaaacag aaaatctcag
      241 cgatcaaaaa ttactacata ttgattgccg tagtacaaat gaaatcaaga taaatatctt
      301 tt
//
LOCUS       DAZFKY010000647          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFKY010000647 DAZFKY010000000
VERSION     DAZFKY010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.652"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..189
                     /locus_tag="ACHKCL_005363"
     CDS             <1..189
                     /locus_tag="ACHKCL_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5766097.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKTARGD"
     gene            194..>301
                     /locus_tag="ACHKCL_005364"
     CDS             194..>301
                     /locus_tag="ACHKCL_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO5766098.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLP"
BASE COUNT           77 a           60 c           90 g           74 t
ORIGIN      
        1 ggtgtgtcat acaccacgtc agcggtaggg acgggatact ggttactgca actgctggac
       61 aaagtctctc cgtcccagtg ggtggcaata ggtgtactgg ggagtctgct gtttggcctg
      121 ctgacgtatc tgactaacct gtatttcaaa atcagggagg accgccgtaa gacggcgcgg
      181 ggagactaaa gcgatgaaga aaaaatacga actggttgtt aaagagataa ataattaccc
      241 ggataagatt gctgttactg tggcacttga aattggcggg catccgtcgt tgttgttgcc
      301 a
//
LOCUS       DAZFKY010000648          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFKY010000648 DAZFKY010000000
VERSION     DAZFKY010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.653"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..>300
                     /locus_tag="ACHKCL_005365"
     CDS             16..>300
                     /locus_tag="ACHKCL_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5766099.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEH"
BASE COUNT           88 a           80 c           73 g           59 t
ORIGIN      
        1 gccacctgat taaaggtgat atcctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gtactttcac ccctgaattc aggctggaat gtgcacagct aattgttgat
      121 aagggctact catatcgaca agccagtgaa gcgatgaatg tcggttctac cacgcttgag
      181 agttgggtgc gccagctcag gcgagagcgt caggggattg cgccctctgc cacacctatt
      241 actccagacc agcaacgtat ccgcgaactg gaaaagcagg ttcgccgcct ggaggaacac
//
LOCUS       DAZFKY010000649          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFKY010000649 DAZFKY010000000
VERSION     DAZFKY010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.654"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>298)
                     /locus_tag="ACHKCL_005366"
     CDS             complement(<1..>298)
                     /locus_tag="ACHKCL_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5766100.1"
                     /translation="NALPNKETISSNFCERLEQWGNKSLNNGEERAIAVERIKEAYNS
                     NMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFTDNASLVNINLSFNKINT"
BASE COUNT           87 a           52 c           59 g          100 t
ORIGIN      
        1 cgtgttaatt ttattaaagc tgaggttgat attaacgaga ctggcattgt cagtaaagtc
       61 aagacaagtt agacaattgt tttccagatt taacgtatta actgtcgagg ggataggcgg
      121 taactcactc aaatcaagat atgacaaatc aagagatgcc atgttcgaat tgtaagcttc
      181 tttaattcgc tccacggcaa ttgctctttc ttcaccatta ttaagcgact tattacccca
      241 ttgttctagt cgttcacaaa aattgctgga gatggtttct ttgttaggta atgcgtta
//
LOCUS       DAZFKY010000650          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFKY010000650 DAZFKY010000000
VERSION     DAZFKY010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.655"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..204
                     /locus_tag="ACHKCL_005367"
     CDS             <1..204
                     /locus_tag="ACHKCL_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5766101.1"
                     /translation="FRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDIT
                     KGATYPVLIDSIAVEVNNTDTAA"
BASE COUNT           82 a           67 c           83 g           66 t
ORIGIN      
        1 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
       61 acaccgttca cgctgacgca gtcaccggtc agtgcatttg cggcggataa actgcatgtg
      121 acagacatta ccaaaggtgc gacttacccg gtgctgattg acagcattgc ggtggaagtg
      181 aacaacacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      241 ggtggtaata cctatggaga aaaaataaag gaacgatact ttcgtgctct ggtttttt
//
LOCUS       DAZFKY010000651          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFKY010000651 DAZFKY010000000
VERSION     DAZFKY010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.656"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..>287
                     /locus_tag="ACHKCL_005368"
     CDS             2..>287
                     /locus_tag="ACHKCL_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766102.1"
                     /translation="MTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDKGRDVVGYK
                     SDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKYFF"
BASE COUNT           83 a           37 c           72 g           95 t
ORIGIN      
        1 attgacatgg gatgatgaac aatgggaggt atttgtccat gattggctta ttgtctgtaa
       61 atcagatgat tacccgtgga gcgaacgttt gggaggagct ggagataaag gtagagacgt
      121 tgttggatat aaatcggatc ctaacgtaga aggttattct tgggataatt atcaatgcaa
      181 actgtacaaa aaaagtttag ggttctctga tgttgtagtt gagtttggaa aacttatcta
      241 ttttactctg aatggtgatt atcccatccc tcagaagtac ttttttg
//
LOCUS       DAZFKY010000652          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFKY010000652 DAZFKY010000000
VERSION     DAZFKY010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.657"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..182)
                     /locus_tag="ACHKCL_005369"
     CDS             complement(<1..182)
                     /locus_tag="ACHKCL_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5766103.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPMNLT"
BASE COUNT           81 a           79 c           45 g           82 t
ORIGIN      
        1 gtcaggttca tcggtgaaac cggagtccgg cgcgccttac gcaaacactc tgctcgtttc
       61 tgtgccgcca cttttctttt ctggtcatca cttagctgta ccatcacttt tgcccatcgt
      121 tcagctgctc tccggtacag tccttttttc tccagacatt ctgccaggtg atcatgtagc
      181 ataagtgacc tccgattatc tacagactgc tatcctgaat ttaccttcct ttaatgaaat
      241 aacaataaaa aataaatcac agaaaaacaa caaaacaaca cacaaaa
//
LOCUS       DAZFKY010000653          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFKY010000653 DAZFKY010000000
VERSION     DAZFKY010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.658"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>285
                     /gene="rfbE"
                     /locus_tag="ACHKCL_005370"
                     /gene_synonym="perA"
     CDS             <1..>285
                     /gene="rfbE"
                     /locus_tag="ACHKCL_005370"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO5766104.1"
                     /translation="GKEKEYVNECLDSTWISSKGNYIQKFENKFAEQNHVQYATTVSN
                     GTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDSDNET"
BASE COUNT          105 a           43 c           56 g           81 t
ORIGIN      
        1 aggaaaagaa aaagaatatg taaatgaatg tctggactca acgtggattt catcaaaagg
       61 aaactatatt cagaagtttg aaaataaatt tgcggaacaa aaccatgtgc aatatgcaac
      121 tactgtaagt aatggaacgg ttgctcttca tttagctttg ttagcgttag gtatatcgga
      181 aggagatgaa gttattgttc caacactgac atatatagca tcagttaatg ctataaaata
      241 cacaggagcc acccccattt tcgttgattc agataatgaa acttg
//
LOCUS       DAZFKY010000654          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFKY010000654 DAZFKY010000000
VERSION     DAZFKY010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.659"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..249)
                     /locus_tag="ACHKCL_005371"
     CDS             complement(<1..249)
                     /locus_tag="ACHKCL_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766105.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTY"
BASE COUNT           84 a           64 c           51 g           86 t
ORIGIN      
        1 ataagtggaa ttggctactc tatagatccc cgtttggtta attccctgct ttcctgacat
       61 acctaacaaa tcaccttcgt cagtaacacg aaatgagtgt atccagtcaa atgatatctt
      121 aacttctgac ttgatcgccg ggcatatcag ggccagttca agcctggcat tcttatagct
      181 taaacctgac agatcggcgt gtttaaaaga caacgaagtc ggcgtgatat ttataaaacg
      241 ttcatccatg ttaacaccct taatatatga gactgtactc aattt
//
LOCUS       DAZFKY010000655          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZFKY010000655 DAZFKY010000000
VERSION     DAZFKY010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.660"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>280
                     /locus_tag="ACHKCL_005372"
     CDS             <1..>280
                     /locus_tag="ACHKCL_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO5766106.1"
                     /translation="LNTLSVYSIDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLF
                     YDKYQVDIEANKLTYTVLFPDTELPVQLNYTSIIYGNHGAKYTFLNSGS"
BASE COUNT           97 a           46 c           47 g           90 t
ORIGIN      
        1 actaaatact ctgtctgttt attcaataga tatgattgca tggagggata aaaataaaac
       61 tgcaaaagaa ccgttaccag ttgatgtaat ccagttaact aattggagga tgcataacac
      121 atgctcttta ttttatgata aatatcaagt tgatattgaa gccaataagt taacgtatac
      181 agtcttattt cctgatacgg agttacccgt tcagctcaat tataccagta ttatttatgg
      241 taatcatggg gcaaaataca ccttcctcaa ttcaggaagt
//
LOCUS       DAZFKY010000656          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZFKY010000656 DAZFKY010000000
VERSION     DAZFKY010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.661"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..100
                     /locus_tag="ACHKCL_005373"
     tRNA            24..100
                     /locus_tag="ACHKCL_005373"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHKCL_005374"
     tRNA            143..218
                     /locus_tag="ACHKCL_005374"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFKY010000657          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZFKY010000657 DAZFKY010000000
VERSION     DAZFKY010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.662"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>273
                     /locus_tag="ACHKCL_005375"
     CDS             <1..>273
                     /locus_tag="ACHKCL_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5766107.1"
                     /translation="IEIPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQ
                     DCNMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGTLNSCSAMH"
BASE COUNT           90 a           49 c           47 g           87 t
ORIGIN      
        1 ctatcgaaat cccacagaca gtgaatggtg gttacgattt ttcacacctt agcctgaaag
       61 gtatcgtgat taaagatgaa gatttatcca attcgaattt tgcaggttgc agactacaaa
      121 acgctatttt ccaggactgt aatatgtata aaacgaattt taatttcgcc ataatggaaa
      181 aaatactttt tgataattgt attctcgatg actcatattt cgctcagata aaaatgactg
      241 acggaactct aaattcatgc tccgctatgc atg
//
LOCUS       DAZFKY010000658          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZFKY010000658 DAZFKY010000000
VERSION     DAZFKY010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.663"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>270
                     /locus_tag="ACHKCL_005376"
     CDS             <1..>270
                     /locus_tag="ACHKCL_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766108.1"
                     /translation="WVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAILNSSRK
                     GELSTWDTIYLHLILVINADSDLIKNDVLLAENIPTADYQFNTFF"
BASE COUNT           87 a           44 c           47 g           92 t
ORIGIN      
        1 cttgggtgat ttttaatatg ataaacaata tatctgatca agcgtcatca tttccatgca
       61 cacaattgaa tcaaagtgat aatttcctcg attcactgag ggaatttttc gcgatattaa
      121 attccagtcg taagggtgag ttgtctacat gggataccat ctacttgcat ctgatattag
      181 taataaatgc tgacagtgat ctcataaaaa atgatgtgtt gctcgcggaa aatattccaa
      241 cagcggatta tcaatttaat acatttttta
//
LOCUS       DAZFKY010000659          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZFKY010000659 DAZFKY010000000
VERSION     DAZFKY010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.664"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>270
                     /locus_tag="ACHKCL_005377"
     CDS             <1..>270
                     /locus_tag="ACHKCL_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766109.1"
                     /translation="AGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTML
                     FSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIA"
BASE COUNT           52 a           72 c           86 g           60 t
ORIGIN      
        1 gggccggaaa gggtggactg cagattgtgc tgggggcggc agccatcgtg ggctctttct
       61 tcactgccgg ggcatcaatg gcgttatggg gttcagccct ggcagccggt ggtttttctg
      121 ccaccacgat gctgttttca cttggagcca gcatgattct gggcggtgtg gcccagatgc
      181 tggccccgaa ggcaaaaaca ccggattacc gcgcaacgga taacggcaga cagaacacgt
      241 acttttcctc gctggataac atgattgccc
//
LOCUS       DAZFKY010000660          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZFKY010000660 DAZFKY010000000
VERSION     DAZFKY010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.665"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           77 a           26 c           54 g          107 t
ORIGIN      
        1 atagcagtct ttgcattgat aactatacag tagtaattta gagtgtgtaa gtttttattg
       61 atggggtata ttttaattaa atataattgg aatgtatttt tattctataa acaatatctt
      121 gggtgaatgg attctatatg ggaaatggta acttttggca gttgttcttg ggtaaagaac
      181 cttacagcaa aaaatctgat tttgttaagg catggttatt gtggtgggct attaccatcc
      241 ttgttctatt tattttaagc ctga
//
LOCUS       DAZFKY010000661          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZFKY010000661 DAZFKY010000000
VERSION     DAZFKY010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.666"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>264)
                     /locus_tag="ACHKCL_005378"
     CDS             complement(<1..>264)
                     /locus_tag="ACHKCL_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766110.1"
                     /translation="QSEINKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGSHKTQ
                     KQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSDN"
BASE COUNT           73 a           60 c           40 g           91 t
ORIGIN      
        1 ttgttgtcag attgtagatc aacgccaaat aacgtcttta catcttccct gagattatag
       61 aatgccgtat caacaagagc ctcgatcgtt tgtttatcaa caattttaat aaactcttcg
      121 tatacttgct tttgcgtctt atggctgccc agcgcccatg aatactcatg ttcttttaaa
      181 accagtatgg cccttaacag acaatagcca tcgccatcta ctggagcgta ttttaatcca
      241 taagttttat taatttcact ctga
//
LOCUS       DAZFKY010000662          260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZFKY010000662 DAZFKY010000000
VERSION     DAZFKY010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.667"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>260
                     /locus_tag="ACHKCL_005379"
     CDS             <1..>260
                     /locus_tag="ACHKCL_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766111.1"
                     /translation="INSLTGLVGIPGLPDKEFALSTPEFMKSKQHKAFSTVFKDVTNE
                     LTSIERLGGLRDKLASNFTIDDSYYTEPKTEDPRFRYVSSNW"
BASE COUNT           82 a           45 c           54 g           79 t
ORIGIN      
        1 tataaattca ttgacaggac tcgtgggtat tccaggtttg cctgataaag agtttgcact
       61 gagcactcct gaatttatga agtctaaaca acataaagca ttttctaccg ttttcaagga
      121 tgttactaac gaactaacaa gcattgaacg tttaggtggg cttcgggata aattagcgtc
      181 gaactttact attgatgatt cttattatac cgagccaaaa acagaggatc cgagattccg
      241 ttacgtaagt agtaattgga
//
LOCUS       DAZFKY010000663          257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZFKY010000663 DAZFKY010000000
VERSION     DAZFKY010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.668"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>257
                     /locus_tag="ACHKCL_005380"
     CDS             <1..>257
                     /locus_tag="ACHKCL_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766112.1"
                     /translation="KFVMNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPD
                     GSTNRYERKSFERKDESSLHLITNKVLACYQREANKEIARL"
BASE COUNT           85 a           50 c           53 g           69 t
ORIGIN      
        1 gaagtttgtt atgaataatc ctgaagggga acgagcggat tcacccaagg tgattataga
       61 aatttcactt tccactatta ctactatggg gactcgtcaa ggacatacag ccattatatt
      121 tccacaacct gatggttcga ctaaccgtta tgaaagaaag tcctttgaaa gaaaagatga
      181 gagttcatta cacctgatta ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa
      241 ggaaatagct cgtctat
//
LOCUS       DAZFKY010000664          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZFKY010000664 DAZFKY010000000
VERSION     DAZFKY010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.669"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>255
                     /locus_tag="ACHKCL_005381"
     CDS             <1..>255
                     /locus_tag="ACHKCL_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5766113.1"
                     /translation="RIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDE
                     YGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRI"
BASE COUNT           58 a           88 c           79 g           30 t
ORIGIN      
        1 tccgcatccc gtatgcgacc gacccggcgg gaaaccggct gccggacccg gagctacacc
       61 cggacagcac gctcacggcg tggccggata accgcatcgc ggaggatgcg cactatgtct
      121 accgacacga tgaatacggc aggctgacgg agaagacgga ccgcatcccg gcgggtgtga
      181 tacggacgga cgacgagcgg acccaccact accactacga cagccagcac cgcctggtgt
      241 tctacacgcg gatac
//
LOCUS       DAZFKY010000665          250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZFKY010000665 DAZFKY010000000
VERSION     DAZFKY010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.670"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..143
                     /locus_tag="ACHKCL_005382"
     CDS             <1..143
                     /locus_tag="ACHKCL_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5766114.1"
                     /translation="NLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT           85 a           43 c           63 g           59 t
ORIGIN      
        1 tgaacctgac cgaaataaaa caagcggtca acagactaca ttctgagttg ggaatgggat
       61 ttgaagagcg gcgggtattc cgacgatata aagggacagg agaacagaat acgtccggaa
      121 acgcgcggtc aaaaaaatgc taaaaaatat ctgagagcgt tattgcctgt taccataaga
      181 aaaagcgact ttagtggtcg ctttttgtgt catatataag tcgtttaagt aaacctgtct
      241 gaacaggtgc
//
LOCUS       DAZFKY010000666          248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZFKY010000666 DAZFKY010000000
VERSION     DAZFKY010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.671"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            126..>248
                     /locus_tag="ACHKCL_005383"
     rRNA            126..>248
                     /locus_tag="ACHKCL_005383"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           78 a           45 c           63 g           62 t
ORIGIN      
        1 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
       61 taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa
      121 cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca
      181 tgcaagtcga acggtaacag gaagaagctt gcttctttgc tgacgagtgg cggacgggtg
      241 agtaatgt
//
LOCUS       DAZFKY010000667          246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZFKY010000667 DAZFKY010000000
VERSION     DAZFKY010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.672"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..176
                     /locus_tag="ACHKCL_005384"
                     /pseudo
     CDS             1..176
                     /locus_tag="ACHKCL_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT           69 a           64 c           60 g           53 t
ORIGIN      
        1 ttcagttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa
       61 acaaagggtt cgcagactgg ggagaaatgt tcggagagca tgtgctggca acagcgatcc
      121 tggaccggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt taccggttca
      181 atattccaga cagactcgtg gttcatttga gcctccatgc tcgccctgaa ctccgggcac
      241 acaata
//
LOCUS       DAZFKY010000668          243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZFKY010000668 DAZFKY010000000
VERSION     DAZFKY010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.673"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            77..>243
                     /locus_tag="ACHKCL_005385"
     CDS             77..>243
                     /locus_tag="ACHKCL_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5766115.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTD"
BASE COUNT           49 a           51 c           93 g           50 t
ORIGIN      
        1 ggcagtcttg tgcagctgat tgaccagcgg gtgcaggcgg tgatgctgtc catgcgacgt
       61 gacggaggaa tgctgagtgg ctgagataaa aacgctgcat ctggtcccgc gtgaagggat
      121 gcaggtgagt gagaagccgt cggtggtgag ggtgcggttt ggtgacggtt atgaacagcg
      181 ccgccccaca gggctgaatc ctcaactgaa gacgtttcag gcggtgttcc gggtgacgga
      241 tga
//
LOCUS       DAZFKY010000669          240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZFKY010000669 DAZFKY010000000
VERSION     DAZFKY010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.674"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>240
                     /locus_tag="ACHKCL_005386"
     CDS             <1..>240
                     /locus_tag="ACHKCL_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5766116.1"
                     /translation="TGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRE
                     NLPPLAGGDALYLQQQNYSLEALSRRDAREDPFAS"
BASE COUNT           58 a           62 c           77 g           43 t
ORIGIN      
        1 aaacggggga aaacgagagt acagaatttg atgtcaccac gctgctgaga atggacagtg
       61 agcggcgcat gaaaacgctg ggggatgcgg tgaaaaatac gcttctcacg cccaatgagg
      121 cccgtaaacg ggagaacctg ccgcccctgg ccggcggtga tgcactgtat cttcagcagc
      181 agaactacag tctggaggcg ctgtcccgtc gtgatgcccg tgaggatccg ttcgcgtctg
//
LOCUS       DAZFKY010000670          240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZFKY010000670 DAZFKY010000000
VERSION     DAZFKY010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.675"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>240
                     /locus_tag="ACHKCL_005387"
     CDS             <1..>240
                     /locus_tag="ACHKCL_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766117.1"
                     /translation="EHETNNIHSAMINNLLQEVDIALGKINDIIDSRNISAPHELANI
                     LTREKILTTREKKGNLISLFDGFTLFHCVGMITFLI"
BASE COUNT           92 a           48 c           37 g           63 t
ORIGIN      
        1 gaacatgaaa ccaataacat tcactcagct atgataaata acctattaca ggaagttgat
       61 atagcccttg gtaaaataaa tgacattata gacagcagaa acatatcagc cccccatgaa
      121 ctggcaaata ttcttacccg cgaaaaaata cttacaacac gggaaaaaaa aggaaacctg
      181 ataagcctgt ttgatggatt cactttattc cattgtgttg gaatgataac ctttcttatc
//
LOCUS       DAZFKY010000671          233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZFKY010000671 DAZFKY010000000
VERSION     DAZFKY010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.676"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..191
                     /gene="etgA"
                     /locus_tag="ACHKCL_005388"
     CDS             <1..191
                     /gene="etgA"
                     /locus_tag="ACHKCL_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO5766118.1"
                     /translation="NISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQYA
                     MKIYKTYNKLKNNEQIID"
BASE COUNT           93 a           36 c           41 g           63 t
ORIGIN      
        1 taaacatatc tgttgctggt tatatcttag cctctaacat aaaatccaga gggaatacat
       61 gggatgcagt tggtgcgtat aatgcaggtt actttaatac accaaatgca gtagaactca
      121 gaaggcaata cgcaatgaaa atatataaaa catataataa attaaagaat aatgagcaaa
      181 ttatcgatta ataatcaagg tctatcgcaa tacgaatcaa ttgtgccaca ggt
//
LOCUS       DAZFKY010000672          231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZFKY010000672 DAZFKY010000000
VERSION     DAZFKY010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.677"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>231
                     /locus_tag="ACHKCL_005389"
     CDS             <1..>231
                     /locus_tag="ACHKCL_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5766119.1"
                     /translation="EKTLTVNTGALLTMSVSVNGGTPPYKYAWKKDGQPVEGQTTDTF
                     SKANAQSGDKGAYTCMVMDSAEQPQSITSDACT"
BASE COUNT           63 a           53 c           68 g           47 t
ORIGIN      
        1 ggagaagaca cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg
       61 gggaacgccg ccttataaat acgcctggaa gaaggatggt cagccggtcg agggacagac
      121 tactgacact ttcagtaaag ccaatgcgca gtcaggtgat aagggggctt atacctgcat
      181 ggtaatggat tctgcagaac agccgcagag cattacctct gatgcgtgta c
//
LOCUS       DAZFKY010000673          231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZFKY010000673 DAZFKY010000000
VERSION     DAZFKY010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.678"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>231
                     /locus_tag="ACHKCL_005390"
     CDS             <1..>231
                     /locus_tag="ACHKCL_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5766120.1"
                     /translation="DKTLTVNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTF
                     SKANTQSGDKGAYTCEVTDSAEQPQSITSDACT"
BASE COUNT           65 a           54 c           67 g           45 t
ORIGIN      
        1 ggataagaca cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgtcaacgg
       61 gggaacgccg ccttataaac acgcctggaa gaaggatggt cagccggtag agggacagac
      121 tactgacact ttcagtaaag ccaatacgca gtcaggtgat aagggggctt atacctgcga
      181 ggtaacggat tctgcagaac agccgcagag cattacctct gatgcgtgta c
//
LOCUS       DAZFKY010000674          225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZFKY010000674 DAZFKY010000000
VERSION     DAZFKY010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.679"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>225
                     /locus_tag="ACHKCL_005391"
     CDS             <1..>225
                     /locus_tag="ACHKCL_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766121.1"
                     /translation="RQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAAVASAPVIRVE
                     QKLDKGIGFARFAKSLAAAKGVRSEALEVAR"
BASE COUNT           42 a           61 c           83 g           39 t
ORIGIN      
        1 cgtcaggtgg atgcgcacct gaagcgcctg cgtgaactgg aagccggtaa ggccgccacg
       61 gcgcagccgg tgaaacaggc cggtaacggg aatgtggccg cggtggcttc tgcgccggtg
      121 atccgtgtgg agcagaaact ggataagggg attggcttcg cccgctttgc caaatcgctg
      181 gctgcggcta aaggcgtccg atctgaagcc ctggaagtgg cccgt
//
LOCUS       DAZFKY010000675          224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZFKY010000675 DAZFKY010000000
VERSION     DAZFKY010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.680"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           84 a           50 c           30 g           60 t
ORIGIN      
        1 aaagagcata aacatatgaa ccggaaattc tatttcaaaa atatcctgtg gggaatgtgc
       61 cccattataa tatccatatt gcatctgata tatgacacac caacagaagc atcgataaaa
      121 gttgacttat ttctgatact cagtggcctg gcccttcctt gttcaatata cctgacaaaa
      181 acaatcacac taaaatacac atccatatca ttctggaaaa aacc
//
LOCUS       DAZFKY010000676          221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZFKY010000676 DAZFKY010000000
VERSION     DAZFKY010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.681"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>221
                     /locus_tag="ACHKCL_005392"
     CDS             <1..>221
                     /locus_tag="ACHKCL_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5766122.1"
                     /translation="VAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSF
                     QGLPVIVSQYVGDQLVLVNAPDIYLADDGG"
BASE COUNT           40 a           62 c           73 g           46 t
ORIGIN      
        1 gtggcagcaa acctgcagcc caccggtgcg gtctggctga tgtccagcac caatgccctg
       61 gcactgtcca tgcgtaaaaa tgcgctgggt cagaaggaat acccggacat gaccctgctg
      121 ggtggctcct tccaggggct gccggtgatt gtctcccagt acgtgggtga ccagctggtg
      181 ctggtgaatg ccccggatat ttatctggcg gatgacggcg g
//
LOCUS       DAZFKY010000677          219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZFKY010000677 DAZFKY010000000
VERSION     DAZFKY010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.682"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>219)
                     /locus_tag="ACHKCL_005393"
     CDS             complement(<1..>219)
                     /locus_tag="ACHKCL_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO5766123.1"
                     /translation="ELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAA
                     SPWSKEDLLKPVNSNQYRYCAKHKFKQKH"
BASE COUNT           60 a           44 c           39 g           76 t
ORIGIN      
        1 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
       61 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgacac
      121 cggatagtca gcccactcat gccaaggttt tgtaggtccc acatagtgta taaacacggt
      181 gtcatcattg actggattat caactttgtc ttttaattc
//
LOCUS       DAZFKY010000678          218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZFKY010000678 DAZFKY010000000
VERSION     DAZFKY010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.683"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>218)
                     /locus_tag="ACHKCL_005394"
     CDS             complement(<1..>218)
                     /locus_tag="ACHKCL_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFO5766124.1"
                     /translation="DYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDFGETHLDFL
                     RQYGDFENAIPVHDTIARVVSCISPAKF"
BASE COUNT           71 a           49 c           36 g           62 t
ORIGIN      
        1 tgaaattttg caggactgat acaggataca actctggcaa tggtatcgtg gacaggaata
       61 gcattttcaa aatcaccata ttgcctcaaa aaatcgagat gcgtttcacc aaaatcctct
      121 atatcttccc aaccttctgc accagaaatg acggcacaaa tagtcaacag tagaatatct
      181 gacaatttat gttctacttt ccaggcttgt ctgtaatc
//
LOCUS       DAZFKY010000679          212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZFKY010000679 DAZFKY010000000
VERSION     DAZFKY010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.684"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>212
                     /locus_tag="ACHKCL_005395"
     CDS             <1..>212
                     /locus_tag="ACHKCL_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO5766125.1"
                     /translation="EYYSHTVDIEKNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYL
                     WVDEKAFGAAKFTSVLKQIAFDLACR"
BASE COUNT           68 a           38 c           41 g           65 t
ORIGIN      
        1 ggagtactat tcgcatacag ttgatattga gaaaaatatt catgccatat gggttgcggg
       61 ttccccacct gaaagcatct cggactatat taagactttt ctcaaaacct acaaagagtt
      121 tacttactat ctttgggttg atgaaaaagc atttggagcc gcgaaattta ccagtgtctt
      181 aaaacaaatt gcatttgatt tagcatgcag aa
//
LOCUS       DAZFKY010000680          212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZFKY010000680 DAZFKY010000000
VERSION     DAZFKY010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.685"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>212
                     /locus_tag="ACHKCL_005396"
     CDS             <1..>212
                     /locus_tag="ACHKCL_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5766126.1"
                     /translation="GFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSERE
                     EIRAGLSAKMSIRAIATALNRSPSTIS"
BASE COUNT           55 a           52 c           57 g           48 t
ORIGIN      
        1 ggcttcagtg aaatagcgaa tatcctgggt tcaaaacccg gaacgatctt cactatgtta
       61 agggatactg gcggcataaa accccatgag cgtaagcggg ctgtagctca cctgacactg
      121 tctgagcgcg aggagatacg agctggtttg tcagccaaaa tgagcattcg tgcgatagct
      181 actgcgctga atcgcagtcc ttcgacgatc tc
//
LOCUS       DAZFKY010000681          209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZFKY010000681 DAZFKY010000000
VERSION     DAZFKY010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.686"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>209
                     /locus_tag="ACHKCL_005397"
     CDS             <1..>209
                     /locus_tag="ACHKCL_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO5766127.1"
                     /translation="TRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDAVIA
                     GQNQAYRELRRIRDNIHSEIYFKQT"
BASE COUNT           69 a           39 c           46 g           55 t
ORIGIN      
        1 tacaaggatt cctcgagaca caatgcccat ccttcgtcgt tatcacctat ggcctgatgg
       61 atggaatatt cgtgggctca atggattcat gctatcacat aaaggtagtg aagtgattga
      121 tgctgttatc gcaggccaga atcaggctta cagggaacta agaagaataa gagataatat
      181 tcatagtgaa atatacttca aacaaactg
//
LOCUS       DAZFKY010000682          207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZFKY010000682 DAZFKY010000000
VERSION     DAZFKY010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.denovo.687"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT           45 a           60 c           45 g           57 t
ORIGIN      
        1 gcaatggcgc tgatgtcaat ataaagcgcc attgctgctt ctttgccgat cccgggatgc
       61 cttccattct gatgtttgac ttcgcccact gagaaaccct ctgtttcccc ttaacgccgg
      121 ggtagcggaa caaaaaactg ctgcatagtt attaaagttg aaccctgccg tcatgttcat
      181 acgcctcggg ctggctactt aacccct
//
LOCUS       DAZFKY010000683        10006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.guided.172, whole genome shotgun sequence.
ACCESSION   DAZFKY010000683 DAZFKY010000000
VERSION     DAZFKY010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.guided.172"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..676)
                     /locus_tag="ACHKCL_005398"
     CDS             complement(<1..676)
                     /locus_tag="ACHKCL_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5766128.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEA"
     gene            complement(895..1872)
                     /gene="epmA"
                     /locus_tag="ACHKCL_005399"
     CDS             complement(895..1872)
                     /gene="epmA"
                     /locus_tag="ACHKCL_005399"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5766129.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2197..4005
                     /gene="frdA"
                     /locus_tag="ACHKCL_005400"
     CDS             2197..4005
                     /gene="frdA"
                     /locus_tag="ACHKCL_005400"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5766130.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3998..4732
                     /gene="frdB"
                     /locus_tag="ACHKCL_005401"
     CDS             3998..4732
                     /gene="frdB"
                     /locus_tag="ACHKCL_005401"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5766131.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPTQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4743..5138
                     /gene="frdC"
                     /locus_tag="ACHKCL_005402"
     CDS             4743..5138
                     /gene="frdC"
                     /locus_tag="ACHKCL_005402"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5766132.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5149..5508
                     /gene="frdD"
                     /locus_tag="ACHKCL_005403"
     CDS             5149..5508
                     /gene="frdD"
                     /locus_tag="ACHKCL_005403"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5766133.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5571..6704
                     /locus_tag="ACHKCL_005404"
     CDS             5571..6704
                     /locus_tag="ACHKCL_005404"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5766134.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6792..7325
                     /gene="blc"
                     /locus_tag="ACHKCL_005405"
     CDS             6792..7325
                     /gene="blc"
                     /locus_tag="ACHKCL_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5766135.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7322..7639)
                     /gene="sugE"
                     /locus_tag="ACHKCL_005406"
     CDS             complement(7322..7639)
                     /gene="sugE"
                     /locus_tag="ACHKCL_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5766136.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7815..7961)
                     /gene="ecnB"
                     /locus_tag="ACHKCL_005407"
     CDS             complement(7815..7961)
                     /gene="ecnB"
                     /locus_tag="ACHKCL_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5766137.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8072..8197)
                     /gene="ecnA"
                     /locus_tag="ACHKCL_005408"
     CDS             complement(8072..8197)
                     /gene="ecnA"
                     /locus_tag="ACHKCL_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5766138.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8249..8815)
                     /gene="efp"
                     /locus_tag="ACHKCL_005409"
     CDS             complement(8249..8815)
                     /gene="efp"
                     /locus_tag="ACHKCL_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5766139.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8857..9885
                     /gene="epmB"
                     /locus_tag="ACHKCL_005410"
     CDS             8857..9885
                     /gene="epmB"
                     /locus_tag="ACHKCL_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5766140.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2569 a         2579 c         2527 g         2331 t
ORIGIN      
        1 ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc attgccagac
       61 cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg aaatgcccac
      121 catttaataa caaaatagta atgcttacta acagcaatac taaattcaga cacattactg
      181 caataccgcc caccgcagta atgcgggcaa ttttattaat cccctttgaa gcaacgacgg
      241 tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc tccagtccgg
      301 caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac ggtacccaaa
      361 ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata aacgtaccaa
      421 tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg ccttcttctt
      481 tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag aataacaatg
      541 cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc gatgggctat
      601 tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga ctcatctttt
      661 ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac gcgcccactg
      721 aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt gatacctgga
      781 tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa acatatgcta
      841 cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc agaattatgc
      901 ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg ccaacatcac
      961 cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt tcaaggcttc
     1021 aatcagattc tgatcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct tacggttatc
     1081 ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac cattcgccag
     1141 ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt cggtactgat
     1201 ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt tttctttacc
     1261 aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc ggtcttcttc
     1321 ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt gcgttttgtc
     1381 ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag aaaggctttc
     1441 tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca tcaaccggta
     1501 catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt gataacgccc
     1561 catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac cggcaaccag
     1621 caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc cctgcgaatg
     1681 cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta ccgtcgcctg
     1741 gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac gacggatctc
     1801 cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct gccaggatgc
     1861 cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc
     1921 gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact aaaaaaagca
     1981 cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc cctcttgcgc
     2041 actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac tgttgtacct
     2101 ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta ccctgaagta
     2161 cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca agccgatctt
     2221 gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat
     2281 ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca taccgttgct
     2341 gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata tcactttcac
     2401 gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta tttcgtccac
     2461 cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg
     2521 gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac ctggttcgcc
     2581 gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct gcaattcccg
     2641 cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga tggtcatgtt
     2701 cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg tgctaacgcg
     2761 gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc
     2821 gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg tgacatggaa
     2881 ttcgttcagt atcatccaac cggtctgcca ggttccggta tcctgatgac cgaaggttgc
     2941 cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca agattacggc
     3001 atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact gggtccacgc
     3061 gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg
     3121 cgtggtgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact gcatgaacgt
     3181 ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg
     3241 attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga tcagaactgt
     3301 gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt
     3361 gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg tctggccggt
     3421 gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc aattgaagcg
     3481 caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg cggcgaaaac
     3541 tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg tatctaccgt
     3601 acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga acgcttcaag
     3661 cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta caccattgaa
     3721 ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc acgtaaagag
     3781 tcccgcggcg cacaccagcg tctggacgaa ggttgcacca agcgtgacga cgtcaacttc
     3841 ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga gtacagcgac
     3901 gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc
     3961 gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga aaaacctgaa
     4021 aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg cattctatga
     4081 agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca aagacaacct
     4141 ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat
     4201 gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg
     4261 tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac
     4321 ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc
     4381 ggatcagggt actaacatcc agaccccgac gcagatggcg aagtatcacc agttctccgg
     4441 ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt
     4501 catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg
     4561 taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt
     4621 gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcaacaggg
     4681 caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca
     4741 acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat
     4801 tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca
     4861 gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat
     4921 tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact ctggcggcag
     4981 ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa
     5041 aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg
     5101 ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca
     5161 aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg
     5221 agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc gctggggctg
     5281 tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt
     5341 cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac
     5401 cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt ctacggtctg
     5461 gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg catcgccaat
     5521 gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct atgttcaaaa
     5581 cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa
     5641 tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta
     5701 tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggg tatgcggaca
     5761 tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa
     5821 catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc
     5881 ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac
     5941 atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct
     6001 caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac
     6061 gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt
     6121 tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt
     6181 ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggcaagg
     6241 cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag tcgaccattg
     6301 aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac gacaaaacac
     6361 ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg
     6421 gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt aacggcagtg
     6481 acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca actcctgcag
     6541 tacgagcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt
     6601 ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag
     6661 cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc atcgggtccg
     6721 aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaccagta
     6781 aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg
     6841 ttgcctgcag ttctcctacg ccgccgcgtg gcgtgaccgt agtgaataat ttcgacgcta
     6901 aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgcttt gaacgtggac
     6961 tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata
     7021 aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg
     7081 gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata
     7141 acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggaccg gaccgcgact
     7201 acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg
     7261 cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta
     7321 gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca
     7381 gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg
     7441 cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc atcgcccagg
     7501 caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc gtcaaacgac
     7561 taaagccgtg ggtatatttc aggcctacgg cccataccac ttccagcaga ccagcaataa
     7621 ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg gtgaaaagac
     7681 gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcgggg
     7741 gaatttcaac tattaccttt gtgactatcg cagaaaaaac aaacggcacg acacagctgt
     7801 cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc gttaccgcca
     7861 tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc
     7921 accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc cttttaggta
     7981 atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa aaatgatgga
     8041 aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgggag atggagttgc
     8101 cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg agcagcgtgc
     8161 tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat aagtgatggt
     8221 gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat tcaccagagc
     8281 gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta accacagcgc
     8341 cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc aggcccggat
     8401 cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc ggctgaccat
     8461 tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcgttg tcgccaattg
     8521 cttttgcgtc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc cagaactcac
     8581 cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca gcggaatcag
     8641 tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt ttaacgcgag
     8701 caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg taaggttcgc
     8761 cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt gccataaggc
     8821 cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt aaccctaaat
     8881 accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa
     8941 cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg caacgccaaa
     9001 aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa aggcaatccg
     9061 gacgatccgc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt cgcgcccgga
     9121 ttctccaccg acccgctgga agaacagcac agcgtagtgc ctggtttgtt gcataaatac
     9181 cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc
     9241 cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca aactgcactt
     9301 gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg cgatccgctg
     9361 atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat cccgcatatc
     9421 aaacgtctgc ggattcacag tcgtctgccg attgtgatcc cggcacgcat cacagatgcg
     9481 ctggttgaac gcttttcaca ttctacgctg caaatcttgc tggtgaatca catcaaccat
     9541 gccaatgaga tagatgaaac attccgtcag gcgatggcta agttgcgccg ggcgggcgtt
     9601 actttgctga accagagcgt tctgttacgt ggtgtgaacg ataacgcaca aacgctggca
     9661 aacctgagta atgcgttgtt cgatgcaggc gtgatgccgt attacctgca tgtgctcgat
     9721 aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca gattatgcgt
     9781 gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga aattggcggc
     9841 gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat taaacaggga
     9901 agtttaatct ttgcccgtta cataaccttt aattattcat atacattgct gttgttactc
     9961 tttatataac caacaccaat aattaacctt tcttttctgt ttatat
//
LOCUS       DAZFKY010000684         5666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.guided.245, whole genome shotgun sequence.
ACCESSION   DAZFKY010000684 DAZFKY010000000
VERSION     DAZFKY010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.guided.245"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..302
                     /locus_tag="ACHKCL_005411"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHKCL_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHKCL_005412"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHKCL_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKCL_005413"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHKCL_005413"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5766141.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKCL_005414"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHKCL_005414"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5766142.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKCL_005415"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHKCL_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5766143.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHKCL_005416"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHKCL_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5766144.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..>5666)
                     /locus_tag="ACHKCL_005417"
     CDS             complement(4889..>5666)
                     /locus_tag="ACHKCL_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5766145.1"
                     /translation="KTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSS
                     AQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTY
                     DFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRK
                     MTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGAT
                     YYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1374 a         1405 c         1469 g         1418 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     5521 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcggtc caggcttcca
     5581 cgtcatacaa cgcgcccagg ttacggccat agtcgaaaga acccaaatct ttatacttca
     5641 atccggcaaa agcgagacgc gttttt
//
LOCUS       DAZFKY010000685         1733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.guided.373, whole genome shotgun sequence.
ACCESSION   DAZFKY010000685 DAZFKY010000000
VERSION     DAZFKY010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.guided.373"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKCL_005418"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKCL_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5766146.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKCL_005419"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKCL_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5766147.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            complement(1546..>1733)
                     /locus_tag="ACHKCL_005420"
     CDS             complement(1546..>1733)
                     /locus_tag="ACHKCL_005420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5766148.1"
                     /translation="LCAIAWCRVPPGEFGLVPPNPRILAYDHQRDHTVHQSPLRTGGF
                     TMQKFSNTSIIRPATTD"
BASE COUNT          462 a          303 c          427 g          541 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgat
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgacttag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
     1561 cggtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
     1621 cgactggtga acggtatgat ctctttgatg atcgtaagcg agaatacgcg ggtttggtgg
     1681 caccaggccg aactcaccgg gaggcacccg gcaccatgca atggcacata gcg
//
LOCUS       DAZFKY010000686         1308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.guided.406, whole genome shotgun sequence.
ACCESSION   DAZFKY010000686 DAZFKY010000000
VERSION     DAZFKY010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.guided.406"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..125
                     /locus_tag="ACHKCL_005421"
     tRNA            49..125
                     /locus_tag="ACHKCL_005421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Arg,seq:tcg)
     gene            139..215
                     /locus_tag="ACHKCL_005422"
     tRNA            139..215
                     /locus_tag="ACHKCL_005422"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:173..175,aa:Arg,seq:tct)
     gene            306..1265
                     /locus_tag="ACHKCL_005423"
     CDS             306..1265
                     /locus_tag="ACHKCL_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO5766149.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
BASE COUNT          335 a          288 c          322 g          363 t
ORIGIN      
        1 atcgccaggt cgctggttca aatccagcaa gggccaccat atcacatacc gccattagct
       61 catcgggaca gagcgccagc cttcgaagct ggctgcgcgg ggttcgagtc ctcgatggcg
      121 gtccattatc tgcattatgc gttgttagct cagccggaca gagcaattgc cttctgagca
      181 atcggtcact ggttcgaatc cagtacaacg cgccatattt atttaccagg ctcgcttttg
      241 cgggcctttt ttatatctgc gccgggtctg gtgctgatta cttcagccaa aaggaacacc
      301 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
      361 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      421 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      481 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      541 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      601 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      661 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      721 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      781 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      841 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      901 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      961 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
     1021 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
     1081 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
     1141 aaccagaatg tcagataact ggcgacaggc ctgttataaa aataaacaat acattatggg
     1201 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
     1261 aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgc
//
LOCUS       DAZFKY010000687          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112F30953
            SAMN43267756-rid23066263.guided.515, whole genome shotgun sequence.
ACCESSION   DAZFKY010000687 DAZFKY010000000
VERSION     DAZFKY010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267756
            Sequence Read Archive: SRR30307540
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350857.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350857.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:50:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 95
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 4 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 91 of 234
            Pseudo Genes (incomplete)         :: 124 of 234
            Pseudo Genes (internal stop)      :: 67 of 234
            Pseudo Genes (multiple problems)  :: 37 of 234
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267756-rid23066263.guided.515"
                     /serotype="O157:H 7"
                     /isolate="2112F30953"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            9..278
                     /locus_tag="ACHKCL_005424"
     CDS             9..278
                     /locus_tag="ACHKCL_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO5766150.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          191 a          146 c          183 g          204 t
ORIGIN      
        1 agttaagcat gaagaagatg tttatggcgg ttttatttgc attagcttct gttaatgcaa
       61 tggcggcgga ttgtgctaaa ggtaaaattg agttttccaa gtataatgag gatgacacat
      121 ttacagtgaa ggttgacggg aaagaatact ggaccagtcg ctggaatctg caaccgttac
      181 tgcaaagtgc tcagttgaca ggaatgactg tcacaatcaa atccagtacc tgtgaatcag
      241 gctccggatt tgctgaagtg cagtttaata atgactgagg cataacctga ttcgtggtat
      301 gtgggtaaca agtgtaatct gtgtcacaat tcagtcagtt gacagttgcc tgtcagactg
      361 agcatttgtt aaaaaaattt cgcatggtga atccccctgt gtggaggggc gactggtgaa
      421 aaatccttgc ttgtgattca ttatcgacac gggttcggtg gtaccaggcc gaactcaccg
      481 ggaggcaccc ggcaccatgc agtatacaga gattaggcat ataccaaggc ctctcatagc
      541 aggggccttt ttacatgtaa aaaagcccga gtgggttcgg gcaattgcat gagatactcg
      601 ttttaataat cgaaatcatt ttaaccagga ttcataaggc tgcgcaactg cgcggccttt
      661 ttcgtatttc gggctgtagt cttccttctg ccattgtcct gtaacttccg gacttcagcc
      721 cgct
//
